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Sample records for anti-mullerian hormone

  1. Anti-Mullerian hormone and ovarian dysfunction

    NARCIS (Netherlands)

    Broekmans, Frank J.; Visser, Jenny A.; Laven, Joop S. E.; Broer, Simone L.; Themmen, Axel P. N.; Fauser, Bart C.

    2008-01-01

    Anti-Mullerian hormone (AMH) has important roles in postnatal ovarian function. Produced by ovarian granulosa cells, AMH is involved in initial follicle development. In fact, serum AMH level correlates with ovarian follicle number. In patients with polycystic ovary syndrome (PCOS), AMH levels are el

  2. Anti-Mullerian hormone normogram in an Irish subfertile population.

    LENUS (Irish Health Repository)

    Naasan, M N

    2014-02-23

    There has been much interest in the use of anti-Mullerian hormone (AMH) as a biomarker in the assessment of ovarian reserve, and debate on its use as a predictor of assisted reproductive technology (ART) outcomes. Normal levels have not been well defined, and age-based reference ranges may have a role in counselling patients.

  3. Anti-Mullerian hormone in health and disease: a review

    Directory of Open Access Journals (Sweden)

    Mangala Sirsikar

    2016-07-01

    Full Text Available Anti-Mullerian hormone (AMH is a homodimeric glycoprotein, member of the transforming growth factor beta family of growth and differentiation factors. In the ovary, AMH has an inhibitory effect on primordial follicle recruitment as well as on the responsiveness of growing follicles to follicle-stimulating hormone (FSH. The ovary-specific expression pattern in granulosa cells of growing non-selected follicles makes AMH an ideal marker for the size of the ovarian follicle pool. AMH levels accurately reflect the ovarian follicular reserve and could, therefore, be considered as an extremely sensitive marker of ovarian aging and a valuable tool in the diagnosis and the recognition of recurrence of granulosa cell tumors. Furthermore, AMH could be a surrogate diagnostic marker of polycystic ovary syndrome in cases in which ultrasonographic examination is not possible. Additionally AMH evaluation is of clinical importance in predicting the success of in vitro fertilization (IVF. Special reference is made to the possible implications of AMH in the pathogenesis of polycystic ovary syndrome and the relationship between AMH and obesity. AMH also plays important role in evaluation of infants with ambiguous genitalia and other intersex conditions. This article is a review of the clinical usefulness of AMH evaluation in the fields of gynecological endocrinology, menopause, gynecological oncology and assisted reproduction and also in pediatric patients. [Int J Res Med Sci 2016; 4(7.000: 2514-2520

  4. Anti-mullerian hormon level and polycystic ovarian syndrome diagnosis

    Directory of Open Access Journals (Sweden)

    Shahrzad Zadehmodarres

    2015-03-01

    Full Text Available Background: Polycystic ovarian syndrome (PCOS is a common endocrinopathy that accompanied with long term complications. The early diagnosis of this syndrome can prevent it. Objective: The aim was to determine the role of anti-mullerian hormon (AMH in PCOS diagnosis and to find cut off level of it. Materials and Methods: In this cross sectional study, 117 women between 20-40 years old were participated in two groups: 60 PCOS women (based on Rotterdam criteria consensus as the case group and 57 normal ovulatory women as the control group. In day 2-4 of cycle, transvaginal sonography was performed and serum hormonal level of AMH, luteinizing hormone (LH, follicle stimulating hormone (FSH, estradiol (E2, testosterone, fasting blood sugar (FBS, thyroid stimulating hormone (TSH, and prolactin (PRL were measured in all of participants. For all of them score of hirsutism (base on Freeman-Galloway scoring was determined. Results: There were statistically significant in irregular pattern of menstruation, AMH and FSH level, and presence of hirsutism between two groups. But regarding mean of age, body mass index, plasma level of PRL, TSH, LH, Testosterone, FBS, and E2 differences were not significant. Construction by ROC curve present 3.15 ng/ml as AMH cut off with 70.37% sensitivity and 77.36% specificity in order to PCOS diagnosis. Conclusion: AMH with cut off level of 3.15 ng/ml with sensitivity 70.37% and specificity 77.36% could use for early diagnosis of PCOS patients.

  5. Anti-Mullerian hormone : a marker for oocyte quantity, oocyte quality and embryo quality?

    NARCIS (Netherlands)

    Fong, S. Lie; Baart, E. B.; Martini, E.; Schipper, I.; Visser, J. A.; Themmen, A. P. N.; de Jong, F. H.; Fauser, B. J. C. M.; Laven, J. S. E.

    2008-01-01

    Serum anti-Mullerian hormone (AMH) concentrations decline with increasing age and constitute a Sensitive marker for ovarian ageing. In addition, basal serum AMH concentrations predict ovarian response during IVF cycles. Concomitantly, oocyte quantity and embryo quality decrease with advancing age. H

  6. Ovarian response prediction in GnRH antagonist treatment for IVF using anti-Mullerian hormone

    NARCIS (Netherlands)

    Hamdine, O.; Eijkemans, M.J.; Lentjes, E.W.; Torrance, H.L.; Macklon, N.S.; Fauser, B.C.; Broekmans, F.J.

    2015-01-01

    STUDY QUESTION: What is the clinical value of anti-Mullerian hormone (AMH) for the prediction of high or low ovarian response in controlled ovarian stimulation for IVF using GnRH antagonist treatment? SUMMARY ANSWER: AMH as a single test has substantial accuracy for ovarian response prediction in Gn

  7. Anti-Mullerian hormone (AMH) : what do we still need to know?

    NARCIS (Netherlands)

    La Marca, A.; Broekmans, F. J.; Volpe, A.; Fauser, B. C.; Macklon, N. S.

    2009-01-01

    In the ovary, Anti-Mullerian hormone (AMH) is produced by the granulosa cells of early developing follicles and inhibits the transition from the primordial to the primary follicular stage. AMH levels can be measured in serum and have been shown to be proportional to the number of small antral follic

  8. Anti-Mullerian hormone and response to ovulation induction with clomiphene citrate in women with polycystic ovary syndrome

    Directory of Open Access Journals (Sweden)

    Mohamed S. Sweed

    2016-03-01

    Conclusions: Anti-Mullerian hormone is a very useful predictor of poor responders to clomiphene citrate among women with polycystic ovary disease. [Int J Reprod Contracept Obstet Gynecol 2016; 5(3.000: 603-608

  9. Anti-Mullerian-hormone levels during pregnancy and postpartum

    OpenAIRE

    Köninger, Angela; Kauth, Alexis; Schmidt, Boerge; Schmidt, Markus; Yerlikaya, Guelen; Kasimir-Bauer, Sabine; Kimmig, Rainer; Birdir, Cahit

    2013-01-01

    Background The number of unintentionally childless couples is increasing as more couples seek to conceive for the first time in the third or fourth decade of the woman’s life. Determination of ovarian reserve is an essential component of infertility assessment. The Anti-Müllerian-Hormone (AMH) seems to be the most reliable predictor of ovarian reserve. In this study we analyzed AMH in a cohort of pregnant women without fertility impairment to determine age-dependent decline and possible AMH f...

  10. Expression of anti-Mullerian hormone receptor on the appendix testis in connection with urological disorders

    Institute of Scientific and Technical Information of China (English)

    Kornél Kistamás; Olga Ruzsnavszky; Andrea Telek; Lívia Kosztka; Ilona Kovács; Beatrix Dienes; László Csernoch

    2013-01-01

    The female internal sex organs develop from the paramesonephric (Mullerian) duct.In male embryos,the regression of the Mullerian duct is caused by the anti-Mullerian hormone (AMH),which plays an important role in the process of testicular descent.The physiological remnant of the Mullerian duct in males is the appendix testis (AT).In our previous study,we presented evidence for the decreased incidence of AT in cryptorchidism with intraoperative surgery.In this report,the expression of the anti-Mullerian hormone receptor type 2 (AMHR2),the specific receptor of AMH,on the AT was investigated in connection with different urological disorders,such as hernia inguinalis,torsion of AT,cysta epididymis,varicocele,hydrocele testis and various forms of undescended testis.The correlation between the age of the patients and the expression of the AMHR2 was also examined.Reverse transcriptase-polymerase chain reaction (RT-PCR) and immunohistochemistry were used to detect the receptor's mRNA and protein levels,respectively.We demonstrate that AMHR2 is expressed in the ATs.Additionally,the presence of this receptor was proven at the mRNA and protein levels.The expression pattern of the receptor correlated with neither the examined urological disorders nor the age of the patients;therefore,the function of the AT remains obscure.

  11. Changes in anti-Mullerian hormone serum concentrations over time suggest delayed ovarian ageing in normogonadotrophic anovulatory infertility

    NARCIS (Netherlands)

    Mulders, AGMGJ; Laven, JSE; Eijkemans, MJC; de Jong, FH; Themmen, APN; Fauser, BCJM

    2004-01-01

    BACKGROUND: Anti-Mullerian hormone (AMH), produced by growing pre-antral and early antral ovarian follicles, has been shown to be a useful marker for ovarian ageing. Serum AMH concentrations are elevated during reproductive life in anovulatory women, especially in those patients exhibiting polycysti

  12. Comparison of anti-mullerian hormone level in non-endometriotic benign ovarian cyst before and after laparoscopic cystectomy

    OpenAIRE

    Sedigheh Amooee; Mahboubeh Gharib; Parsa Ravanfar

    2015-01-01

    Background: Benign ovarian cysts are common among both pre- and postmenstrual women. Surgical intervention for excision of an ovarian cyst is mandated when symptomatic, or chance for malignancy is high. The damaging effect of surgical ovarian cystectomy on ovarian reserve is debated in recent studies. Objective: In the present study we investigated serum level of anti-mullerian hormone (AMH) as an indicator of ovarian reserve before and after surgical cystectomy. Materials and Methods: ...

  13. Anti-Mullerian Hormone: Above and Beyond Conventional Ovarian Reserve Markers

    Science.gov (United States)

    Jamil, Zehra; Fatima, Syeda Sadia; Ahmed, Khalid; Malik, Rabia

    2016-01-01

    Management of ovarian dysfunctions requires accurate estimation of ovarian reserve (OR). Therefore, reproductive hormones and antral follicle count (AFC) are assessed to indicate OR. Serum anti-Mullerian hormone (AMH) is a unique biomarker that has a critical role in folliculogenesis as well as steroidogenesis within ovaries. Secretion from preantral and early antral follicles renders AMH as the earliest marker to show OR decline. In this review we discuss the dynamics of circulating AMH that remarkably vary with sex and age. As it emerges as a marker of gonadal development and reproductive disorders, here we summarize the role of AMH in female reproductive physiology and provide evidence of higher accuracy in predicting ovarian response to stimulation. Further, we attempt to compile potential clinical applications in children and adults. We propose that AMH evaluation has a potential role in effectively monitoring chemotherapy and pelvic radiation induced ovarian toxicity. Furthermore, AMH guided ovarian stimulation can lead to individualization of therapeutic strategies for infertility treatment. However future research on AMH levels within follicular fluid may pave the way to establish it as a marker of “quality” besides “quantity” of the growing follicles. PMID:26977116

  14. Anti-Mullerian Hormone: Above and Beyond Conventional Ovarian Reserve Markers

    Directory of Open Access Journals (Sweden)

    Zehra Jamil

    2016-01-01

    Full Text Available Management of ovarian dysfunctions requires accurate estimation of ovarian reserve (OR. Therefore, reproductive hormones and antral follicle count (AFC are assessed to indicate OR. Serum anti-Mullerian hormone (AMH is a unique biomarker that has a critical role in folliculogenesis as well as steroidogenesis within ovaries. Secretion from preantral and early antral follicles renders AMH as the earliest marker to show OR decline. In this review we discuss the dynamics of circulating AMH that remarkably vary with sex and age. As it emerges as a marker of gonadal development and reproductive disorders, here we summarize the role of AMH in female reproductive physiology and provide evidence of higher accuracy in predicting ovarian response to stimulation. Further, we attempt to compile potential clinical applications in children and adults. We propose that AMH evaluation has a potential role in effectively monitoring chemotherapy and pelvic radiation induced ovarian toxicity. Furthermore, AMH guided ovarian stimulation can lead to individualization of therapeutic strategies for infertility treatment. However future research on AMH levels within follicular fluid may pave the way to establish it as a marker of “quality” besides “quantity” of the growing follicles.

  15. Change of anti-Mullerian-hormone levels during follicular phase in PCOS patients.

    Science.gov (United States)

    Köninger, A; Koch, L; Enekwe, A; Birdir, C; Kasimir-Bauer, S; Kimmig, R; Strowitzki, T; Schmidt, B

    2015-01-01

    Anti-Mullerian-hormone (AMH) does not seem to fluctuate significantly during the menstrual cycle in healthy women. However, little is known about cycle fluctuations of AMH levels in patients with polycystic ovarian syndrome (PCOS). The purpose of this study was to examine AMH fluctuations during the follicular phase in PCOS patients receiving antiestrogens or recombinant follicle-stimulating hormone (FSH). About 40 PCOS patients diagnosed according to Rotterdam ESHRE/ASRM-Sponsored PCOS Consensus Workshop Group 2003 and 19 controls were prospectively enrolled. PCOS patients received either antiestrogens or recombinant FSH for monoovulation induction and controls received antiestrogens. AMH levels were determined (1) between the 2nd and the 5th day of follicular phase and (2) when a single large dominant follicle ≥18 mm had appeared. Our study shows that AMH levels do not change during follicular development in controls as well as in PCOS patients with AMH levels PCOS patients with AMH levels ≥5 ng/ml, AMH declines significantly during follicular development (p PCOS patients without the influence of antiestrogens or exogenous FSH, because these interventions may lower AMH values in patients with high levels.

  16. Anti-Mullerian hormone and antral follicle count as predictors of ovarian reserve and successful IVF

    Institute of Scientific and Technical Information of China (English)

    Ibrahim A Abdelazim; Maha M Belal; Hanan H Makhlouf

    2012-01-01

    Objective:To investigate the role ofAnti-Mullerian hormone and antral follicle count in predicting the ovarian reserve, and success ofIVF.Methods:Ninety two infertile couples complaining of infertility due to male or tubal or unexplained factors were included in this comparative prospective study forIVF/ICSI.Day-3 basal hormonal level ofFSH,LH,E2, and AMH were measured, followed byTransvaginal ultrasound(TVS) to evaluate theAFC.Controlled ovarian hyperstimulation was done using the long protocol for ovarian hyperstimulation.Results:According to the number of retrieved oocytes women included in this study were classified into two groups; good responders(≥4 retrieved oocytes) and poor responders(<4 retrieved oocytes). Ovarian reserve in this study was assessed by day-3 basal hormonal levels andAFC.The mean Day-3AMH and meanAFC were significantly high(4.93±1.22) ng/mL, and(12.72±5.70) ng/mL; respectively) in good responders compared with poor responders, also, the number of retrieved oocytes were significantly high in the good responders group compared with poor responders (13.52±9.70) versus(3.91±1.20)(P<0.05).The numbers of chemical and clinical pregnancies were significantly high(6 cases(75%) and13 cases(72.2%); respectively) in the good responders compared with poor responders(2 cases(25%) and5 cases(27.8%); respectively).Conclusions:Day-3AMH andAFC are good predictors for ovarian reserve, there were positively correlated with the number of retrieved oocytes and numbers of chemical and clinical pregnancies.

  17. Expression and regulation of anti-mullerian hormone in an oviparous species, the hen.

    Science.gov (United States)

    Johnson, P A; Kent, T R; Urick, M E; Giles, J R

    2008-01-01

    Anti-mullerian hormone (AMH) has a critical role in regression of the mullerian duct system during development in male mammalian and avian species and in regression of the right oviduct in female avian species. AMH in adult female birds has not been investigated. Chicken-specific cDNA primers were used to isolate Amh by RT-PCR. This probe was used in Northern blot analysis to identify a 2.8-kb band with expression in total ovarian RNA and in granulosa cell RNA. Quantitative real-time PCR was used to assess Amh expression in follicles of different maturity (1, 3, 5, and 6-12 mm and the largest F1 follicle; n = 4-6 of each size). There was an increased amount of Amh mRNA in the granulosa layer of the smaller follicles and a lower amount in the granulosa layer of the larger follicles (P expression between the germinal disc and non-germinal disc region of 6- to 12-mm follicles, although expression differed with follicle size (P estradiol (E(2)) and progesterone (P(4)), and Amh mRNA was assessed. Neither E(2) nor P(4) influenced Amh mRNA accumulation. Granulosa cells were also cultured in the presence of oocyte-conditioned medium (OCM), which decreased Amh mRNA expression in a dose-related manner (P receptor expression was not affected. Heat treatment of OCM abolished the effect, but growth differentiation factor 9 antiserum did not block the suppression. Immunohistochemistry confirmed that the granulosa layer was the predominant source of AMH in the small follicles of the hen and indicated that AMH was present early in follicle development, with expression in very small follicles (approximately 150 mum). PMID:17881771

  18. Anti-mullerian hormone and antral follicle count as predictors of ovarian response in assisted reproduction

    Directory of Open Access Journals (Sweden)

    Y Himabindu

    2013-01-01

    Full Text Available Objective: The objective of this study was to test the hypothesis that AMH and antral follicle count (AFC are good predictors of ovarian response to controlled ovarian stimulation and to compare them. Materials and Methods: This observational cross-sectional study included 56 subjects aged between 25 and 42 years who were enrolled between 1 st January and 31 st December 2010 for their first intracytoplasmic sperm injection (ICSI program. Baseline hormone profiles including serum levels of Estradiol (E2, Follicle-stimulating hormone (FSH, Luteinizing hormone (LH, and Anti-mullerian Hormone (AMH were determined on day 3 of the previous cycle. The antral follicle count measurements were performed on days 3-5 of the same menstrual cycle. Antral follicles within the bilateral ovaries between 2-6 mm were recorded. The subjects were treated with long protocol for ovarian stimulation. Ovulation was induced with 10,000 IU of human chorionic gonadotropin (hCG when at least 3 follicles attained the size of more than 17 mm. Transvaginal oocyte retrieval was performed under ultrasound guidance 36 hours after hCG administration. An oocyte count less than 4 and absence of follicular growth with controlled ovarian hyper stimulation was considered as poor ovarian response. Oocyte count of 4 or more was considered as normal ovarian response. Results: Statistical analysis was performed using SPSS software trail version 16.0. Subjects were divided into 2 groups, depending on the ovarian response. The mean oocyte counts were 12.27 ± 6.06 and 2.22 ± 1.24 in normal and poor responders, respectively, ( P = 001. Multiple regression analysis revealed AMH and antral follicle count as predictors of ovarian response (β coefficient ± SE for AMH was 1.618 ± 0.602 ( P = 0.01 and for AFC, it was, 0.528 ± 0.175 ( P = 0.004. AFC was found to be a better predictor of ovarian response compared to AMH in controlled ovarian hyper stimulation. Conclusion: The observations made

  19. Concentration of anti-Mullerian hormone and inhibin-B in relation to steroids and age in follicular fluid from small antral human follicles

    DEFF Research Database (Denmark)

    Andersen, Claus Yding; Rosendahl, M.; Byskov, A.G.

    2008-01-01

    CONTEXT: Ovaries surgically removed for fertility preservation served as a source of follicle fluid from human small antral follicles. OBJECTIVE: The objective of the study was to measure intrafollicular concentrations of anti-Mullerian Hormone (AMH), inhibin-B, progesterone, androstenedione, tes...

  20. Evaluation of Ovarian Reserve by Measurement of the Serum Levels of Anti-Mullerian Hormone and Follicle-Stimulating Hormone in Intracytoplasmic Sperm Injection Cycles

    OpenAIRE

    Roshan Nikbakht; Mehrdad Borhani; Gita Yazdani Sarvestani

    2010-01-01

    Background: It is important to evaluate ovarian reserves prior to intracytoplasmic sperm injection(ICSI) treatment. The aim of this study is to determine the accuracy of anti-mullerian hormone(AMH) as a marker for ovarian reserve and to compare it with day-3 serum follicle-stimulatinghormone (FSH) levels.Materials and Methods: In this analytic, corss-sectional study, sequential sampling was done on70 infertile women who underwent ICSI treatment at Imam Khomeini Hospital, Ahvaz, Iran.Initially...

  1. Anti Mullerian Hormone: Ovarian response indicator in young patients receiving Long GnRH Agonist Protocol for Ovarian Stimulation

    Science.gov (United States)

    Jamil, Zehra; Fatima, Syeda Sadia; Rehman, Rehana; Alam, Faiza; Arif, Sara

    2016-01-01

    Objective: Anti Mullerian hormone (AMH) is gaining place as ovarian marker, chiefly in infertility assistance. We explored its correlation with oocytes retrieval after long GnRH agonist protocol for stimulation, in younger and older infertile population. Methods: This retrospective analysis compiled data of 166 females, receiving ICSI treatment from June 2014 to March 2015. Serum FSH, LH, Estadiol, AMH and antral follicle count were assessed. Outcomes were measured as good (5 to 19 oocytes) and bad responders. Results: Higher discriminatory power of AMH (AUROC; 0.771; p 35 year (r=0.169; p>0.05). Conclusion: Our study reaffirms that serum AMH correlates well with oocytes retrieved, particularly in females younger than 35 years. We suggest incorporation of AMH in baseline assessment of infertile females, who are falsely advised to postpone interventions based on their age and normal FSH levels. PMID:27648045

  2. Serum anti mullerian hormone levels: A better hormonal marker of ovarian reserve

    Directory of Open Access Journals (Sweden)

    RadhaVembu, Sanjeeva Reddy Nellapalli, Anjalakshi Chandrashekar, Nalini Ganesan R

    2014-04-01

    Full Text Available Aim: To determine whether Serum AMH is a better hormonal marker of Ovarian Reserve. Objectives: 1. To correlate AMH with FSH and maternal Age and whether AMH is a better predictor of ovarian response than FSH. Materials & Methods: A total of 246 women enrolled for IVF-ICSI fulfilling the selection criteria were recruited for the study at a tertiary ART centre. On day 3 of the cycle serum AMH, FSH were assayed along with LH, E2, TSH and Prolactin. Within 3 months they were subjected to IVF-ICSI. Serum AMH and FSH levels were compared with Age and Oocytes retrieved. Results: All the 246 women enrolled were analysed. The mean age of the women was 30.7 ± 4.5, average number of oocytes retrieved was 11.8 ±7.1. There was a negative correlation of AMH with age (r= -0.28 which is statistically significant where as FSH showed a positive correlation (r= -0.27. With regard to retrieval of mature oocytes, AMH showed a high positive correlation (r= 0.60 which is statistically significant (p <0.000 when compared to serum FSH (r = -0.26. Conclusion: AMH is a better hormonal marker of Ovarian Reserve and a better predictor of Oocytes retrieved than serum FSH levels.

  3. Can anti-Mullerian hormone replace ultrasonographic evaluation in polycystic ovary syndrome? A review of current progress

    Directory of Open Access Journals (Sweden)

    Awadhesh Kumar Singh

    2015-01-01

    Full Text Available Several studies over the past decade have now consistently indicated that the serum anti-Mullerian hormone (AMH levels are at least 2–3-fold higher in the patients with polycystic ovary syndrome (PCOS, which also corresponds to the increased number of AMH producing preantral and small antral follicles. Moreover, AMH levels have been found to be associated in direct proportion to the follicle numbers per ovary or antral follicular count, assessed by the transvaginal ultrasound (TVS. Furthermore, AMH correlates directly with the rising serum testosterone and luteinizing hormone levels in PCOS. Hence, serum AMH in women with oligo-anovulation and/or hyperandrogenemia could indicate the presence of underlying PCOS, when reliable TVS is not feasible, or not acceptable, either due to the virginal status or psycho-social issue. In addition, the imaging quality of abdominal ultrasound is often impaired by obesity, which typically occurs in PCOS women. Indeed, PCOS occurs most commonly in young females who cannot be subjected to invasive TVS for various reasons; therefore, a desirable alternative to TVS is urgently required to diagnose the most prevalent endocrine abnormality of young women. This review will analyze the currently available evidence regarding the role of AMH in the diagnosis of PCOS.

  4. Age-Related Distribution of Basal Anti-Mullerian Hormone Levels in a Population of Infertile Women

    Directory of Open Access Journals (Sweden)

    Pinar Ozcan

    2014-12-01

    Full Text Available Aim: We aimed to constitute age-specific reference serum values for anti-Mullerian Hormone (AMH in women, and to analyze the distribution of basal serum AMH levels in Turkish women of reproductive age attending an infertility clinic to provide a framework for expected values according to age. Material and Method: Retrospective analysis of prospectively collected data on cycle day 2-3 serum AMH measurements of 409 women attending a single infertility unit in Turkey through a 12-month-period was performed. Results: Concentrations of serum AMH were shown to decrease with advancing age of the female partner. The mean age of the women was 34.04±5.39 years and the mean AMH level of the women was 1.77±1.82. The AMH levels were grouped according to age as follows: 20-24, 25-29, 30-34,35-39, and >40 years. The median AMH values were 2.16 ng/ml, 2.15 ng/ml, 1.71 ng/ml, 0.80 ng/ml, and 0.47 ng/ml, respectively according to the age groups. Discussion: The present data provide a framework for age-specific serum AMH levels in a Turkish population of infertile women.

  5. 抗苗勒管激素及其Ⅱ型受体与卵母细胞成熟的关系%Relationship between anti-mullerian hormone, anti-mullerian hormone receptor type Ⅱand oocyte maturation

    Institute of Scientific and Technical Information of China (English)

    姜宙; 王晓燕; 孙贤华; 邹淑花

    2013-01-01

    Objective:To investigate the relationship among anti-mullerian hormone (AMH),anti-mullerian hormone receptor type Ⅱ expression and oocyte maturation.Methods:Cumulus granular cells,follicular fluids and blood samples were obtained from 32 ovulatory women undergoing intracytoplasmic sperm injection treatment on ovum pick-up day.All the samples were recorded oocyte maturation and the number of retrieved oocytes.The influence of serum and follicular fluids AMH basal levels were evaluated by ELISA.Cumulus GC were assayed by Realtime PCR for AMHR Ⅱ mRNA expression.Results:AMH concentrations in the FF were higher than that of serum and showed significant differences [(3083.78 ±682.19)pg/ml vs (1012.29±423.21) pg/ml,P<0.001].AMH concentrations in the FF and serum level of each retrieved oocytes were found positive correlation(P=0.045).AMH concentrations in the FF of M Ⅱ oocytes were significantly lower than in the FF of M Ⅰ oocytes (P =0.000).AMHR Ⅱ mRNA expression in cumulus GC in M Ⅰ oocytes were 4 times more than M Ⅱ oocytes (P =0.000).Conclusion:AMH may inhibit the maturation of oocyte via type Ⅱ receptor.Serum AMH level of each retrieved oocytes could be used as a diagnostic criterion of oocyte maturation.%目的:探讨抗苗勒管激素(AMH)及其Ⅱ型受体(AMHⅡ)与卵母细胞成熟的关系.方法:选取因男性因素不孕进行卵胞浆内单精子注射(ICSI)治疗的32例患者,于取卵日采集3ml静脉血,收集卵泡液及卵丘颗粒细胞,记录获卵数、卵子成熟度.采用ELISA法测定血清及卵泡液中的AMH水平;Real-time PCR测定卵丘颗粒细胞中AMHⅡ型受体(AMHRⅡ)mRNA的相对表达.结果:卵泡液中的AMH水平[(3083.78±682.19)pg/ml]显著高于血清[(1012.29±423.21) pg/ml] (P<0.001);卵泡液中AMH水平与血清AMH水平/获卵数呈正相关(P=0.045).MⅠ期卵母细胞卵泡液中的AMH水平显著高于MⅡ期(P=0.000);MⅠ期卵丘颗粒细胞中的AMHRⅡmRNA的

  6. Effect of anti-Mullerian hormone in culture medium on quality of mouse oocytes matured in vitro.

    Directory of Open Access Journals (Sweden)

    Yihui Zhang

    Full Text Available Anti-mullerian hormone (AMH is thought to reflect the growth of follicles and the ovarian function. However, the role of AMH in culture medium during in vitro maturation (IVM on oocyte quality and subsequent development potential is unclear. The objective of this study is to investigate the effect of recombinant human AMH (rh-AMH supplemented into IVM medium on oocyte quality. Cumulus-oocyte complexes (COCs were obtained from ICR mice and cultured in vitro with the different concentrations (0-1,000 ng/ml of rh-AMH. Following 16-18 h of culture, quantitative PCR and ELISA were performed to analyze GDF9 and BMP15 mRNA expression and protein production from the oocytes. Subsequently, in vitro fertilization (IVF and early embryonic development were employed to further evaluate the quality of in vitro matured oocytes. The results showed that AMH was only expressed in cumulus cells but not in the oocytes. However, AMH most specific receptor, AMHR-II, was expressed in both oocytes and cumulus cells. The levels of GDF9 and BMP15 expression and blastocyst formation rate were significantly increased (p<0.05 when the IVM medium was supplemented with 100 ng/ml of rh-AMH. With AdH1-SiRNA/AMH for knocking down of AMH expression during IVM significantly reduced (p<0.05 the levels of GDF9 and BMP15 expression and blastocysts formation rate. These results suggest that AHM improves oocytes quality by up-regulating GDF9 and BMP15 expressions during IVM.

  7. Anti-Mullerian Hormone as A Predictive Factor in Assisted Reproductive Technique of Polycystic Ovary Syndrome Patients

    Directory of Open Access Journals (Sweden)

    Parvin Fallahi

    2011-11-01

    Full Text Available This study aimed to assess the relationship between the serum levels of anti-mullerian hormone (AMH and other hormonal markers and results of assisted reproductive techniques (ART in polycystic ovary syndrome (PCOS patients. This cohort study was conducted on 60 PCOS patients who were candidates for assisted reproductive techniques. In all patients the serum levels of AMH, follicle stimulating hormone (FSH and luteinizing hormone (LH, estradiol (E2, free testosterone (fT, testosterone (T and inhibin B were measured in the 3rd day of menstrual cycle. The relationship between serum level of measured hormonal markers with retrieved oocytes, mature oocytes, the number of transferred fetus and pregnancy rate were assessed. The cut-off value for the serum level of AMH and retrieved oocytes were determined. There was a significant direct correlation between the serum mullerian inhibiting substance (MIS level with number of total picked up oocytes (r=0.412, mature oocytes (r=0.472 and embryo transfer (r=0.291. There was a linear and significant correlation between inhibin B and fertilization (r=0.283 Cut-off point for AMH level according to presence or absence of pregnancy was 4.8 ng/ml and it was not statistically significant (P=0.655. Area under curve (AUC was 0.543. Cut-off point for MIS according to picked up oocytes was 2.7 ng/ml with area under the curve (ROC curve of 0.724 (CI= 0.591-0.831 (P=0.002. Patients with PCOS who had AMH more than 2.7 ng/ml, the number of retrieved oocytes (6 or more was higher than MIS/AMH <2.7 ng/ml (P=0.002. As a marker of ovarian responsiveness to controlled ovarian hyperstimulation (COH and despite a small sample size of our study, it is revealed that pretreatment MIS/AMH is highly associated with the number of mature oocytes retrieved during COH in PCOS women.

  8. Ovarian antral follicle subclasses and anti-mullerian hormone during normal reproductive aging

    DEFF Research Database (Denmark)

    Bentzen, J G; Forman, J L; Johannsen, T H;

    2013-01-01

    included. Interventions: There were no interventions. MAIN OUTCOME MEASURES: Serum anti-Müllerian hormone (AMH) concentration, antral follicle count (AFC), antral follicle size categories (small: 2-4 mm; intermediate: 5-7 mm; and large: 8-10 mm), and ovarian volume were measured. RESULTS: Serum AMH level...

  9. The physiology and clinical utility of anti-Mullerian hormone in women

    DEFF Research Database (Denmark)

    Dewailly, Didier; Andersen, Claus Yding; Balen, Adam;

    2014-01-01

    BACKGROUND The measurement of circulating anti-Müllerian hormone (AMH) has been applied to a wide array of clinical applications, mainly based on its ability to reflect the number of antral and pre-antral follicles present in the ovaries. AMH has been suggested to predict the ovarian response to ...

  10. Anti-mullerian hormone is not associated with cardiometabolic risk factors in adolescent females.

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    Emma L Anderson

    Full Text Available Epidemiological evidence for associations of Anti-Müllerian hormone (AMH with cardiometabolic risk factors is lacking. Existing evidence comes from small studies in select adult populations, and findings are conflicting. We aimed to assess whether AMH is associated with cardiometabolic risk factors in a general population of adolescent females.AMH, fasting insulin, glucose, HDLc, LDLc, triglycerides and C-reactive protein (CRP were measured at a mean age 15.5 years in 1,308 female participants in the Avon Longitudinal Study of Parents and Children (ALSPAC. Multivariable linear regression was used to examine associations of AMH with these cardiometabolic outcomes.AMH values ranged from 0.16-35.84 ng/ml and median AMH was 3.57 ng/ml (IQR: 2.41, 5.49. For females classified as post-pubertal (n = 848 at the time of assessment median (IQR AMH was 3.81 ng/ml (2.55, 5.82 compared with 3.25 ng/ml (2.23, 5.05 in those classed as early pubertal (n = 460, P≤0.001. After adjusting for birth weight, gestational age, pubertal stage, age, ethnicity, socioeconomic position, adiposity and use of hormonal contraceptives, there were no associations with any of the cardiometabolic outcomes. For example fasting insulin changed by 0% per doubling of AMH (95%CI: -3%,+2% p  = 0.70, with identical results if HOMA-IR was used. Results were similar after additional adjustment for smoking, physical activity and age at menarche, after exclusion of 3% of females with the highest AMH values, after excluding those that had not started menarche and after excluding those using hormonal contraceptives.Our results suggest that in healthy adolescent females, AMH is not associated with cardiometabolic risk factors.

  11. Inhibitory actions of Anti-Mullerian Hormone (AMH on ovarian primordial follicle assembly.

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    Eric E Nilsson

    Full Text Available The current study was designed to investigate the actions of Anti-Müllerian Hormone (AMH on primordial follicle assembly. Ovarian primordial follicles develop from the breakdown of oocyte nests during fetal development for the human and immediately after birth in rodents. AMH was found to inhibit primordial follicle assembly and decrease the initial primordial follicle pool size in a rat ovarian organ culture. The AMH expression was found to be primarily in the stromal tissue of the ovaries at this period of development, suggesting a stromal-epithelial cell interaction for primordial follicle assembly. AMH was found to promote alterations in the ovarian transcriptome during primordial follicle assembly with over 200 genes with altered expression. A gene network was identified suggesting a potential central role for the Fgf2/Nudt6 antisense transcript in the follicle assembly process. A number of signal transduction pathways are regulated by AMH actions on the ovarian transcriptome, in particular the transforming growth factor-beta (TGFß signaling process. AMH is the first hormone/protein shown to have an inhibitory action on primordial follicle assembly. Due to the critical role of the primordial follicle pool size for female reproduction, elucidation of factors, such as AMH, that regulate the assembly process will provide insights into potential therapeutics to manipulate the pool size and female reproduction.

  12. Anti-Mullerian hormone recruits BMPR-IA in immature granulosa cells.

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    Lauriane Sèdes

    Full Text Available Anti-Müllerian hormone (AMH is a member of the TGF-β superfamily secreted by the gonads of both sexes. This hormone is primarily known for its role in the regression of the Müllerian ducts in male fetuses. In females, AMH is expressed in granulosa cells of developing follicles. Like other members of the TGF-β superfamily, AMH transduces its signal through two transmembrane serine/threonine kinase receptors including a well characterized type II receptor, AMHR-II. The complete signalling pathway of AMH involving Smads proteins and the type I receptor is well known in the Müllerian duct and in Sertoli and Leydig cells but not in granulosa cells. In addition, few AMH target genes have been identified in these cells. Finally, while several co-receptors have been reported for members of the TGF-β superfamily, none have been described for AMH. Here, we have shown that none of the Bone Morphogenetic Proteins (BMPs co-receptors, Repulsive guidance molecules (RGMs, were essential for AMH signalling. We also demonstrated that the main Smad proteins used by AMH in granulosa cells were Smad 1 and Smad 5. Like for the other AMH target cells, the most important type I receptor for AMH in these cells was BMPR-IA. Finally, we have identified a new AMH target gene, Id3, which could be involved in the effects of AMH on the differentiation of granulosa cells and its other target cells.

  13. A validated model of serum anti-mullerian hormone from conception to menopause.

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    Thomas W Kelsey

    Full Text Available BACKGROUND: Anti-Müllerian hormone (AMH is a product of growing ovarian follicles. The concentration of AMH in blood may also reflect the non-growing follicle (NGF population, i.e. the ovarian reserve, and be of value in predicting reproductive lifespan. A full description of AMH production up to the menopause has not been previously reported. METHODOLOGY/PRINCIPAL FINDINGS: By searching the published literature for AMH concentrations in healthy pre-menopausal females, and using our own data (combined n = 3,260 we have generated and robustly validated the first model of AMH concentration from conception to menopause. This model shows that 34% of the variation in AMH is due to age alone. We have shown that AMH peaks at age 24.5 years, followed by a decline to the menopause. We have also shown that there is a neonatal peak and a potential pre-pubertal peak. Our model allows us to generate normative data at all ages. CONCLUSIONS/SIGNIFICANCE: These data highlight key inflection points in ovarian follicle dynamics. This first validated model of circulating AMH in healthy females describes a transition period in early adulthood, after which AMH reflects the progressive loss of the NGF pool. The existence of a neonatal increase in gonadal activity is confirmed for females. An improved understanding of the relationship between circulating AMH and age will lead to more accurate assessment of ovarian reserve for the individual woman.

  14. Bivariate analysis of basal serum anti-Mullerian hormone measurements and human blastocyst development after IVF

    LENUS (Irish Health Repository)

    Sills, E Scott

    2011-12-02

    Abstract Background To report on relationships among baseline serum anti-Müllerian hormone (AMH) measurements, blastocyst development and other selected embryology parameters observed in non-donor oocyte IVF cycles. Methods Pre-treatment AMH was measured in patients undergoing IVF (n = 79) and retrospectively correlated to in vitro embryo development noted during culture. Results Mean (+\\/- SD) age for study patients in this study group was 36.3 ± 4.0 (range = 28-45) yrs, and mean (+\\/- SD) terminal serum estradiol during IVF was 5929 +\\/- 4056 pmol\\/l. A moderate positive correlation (0.49; 95% CI 0.31 to 0.65) was noted between basal serum AMH and number of MII oocytes retrieved. Similarly, a moderate positive correlation (0.44) was observed between serum AMH and number of early cleavage-stage embryos (95% CI 0.24 to 0.61), suggesting a relationship between serum AMH and embryo development in IVF. Of note, serum AMH levels at baseline were significantly different for patients who did and did not undergo blastocyst transfer (15.6 vs. 10.9 pmol\\/l; p = 0.029). Conclusions While serum AMH has found increasing application as a predictor of ovarian reserve for patients prior to IVF, its roles to estimate in vitro embryo morphology and potential to advance to blastocyst stage have not been extensively investigated. These data suggest that baseline serum AMH determinations can help forecast blastocyst developmental during IVF. Serum AMH measured before treatment may assist patients, clinicians and embryologists as scheduling of embryo transfer is outlined. Additional studies are needed to confirm these correlations and to better define the role of baseline serum AMH level in the prediction of blastocyst formation.

  15. Assessment of the relationship of basal serum anti-mullerian hormone levels with oocyte quality and pregnancy outcomes in patients undergoing ICSI

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    Gültekin Adanaş Aydın

    2015-03-01

    Full Text Available Background: Anti-Mullerian hormone (AMH is constantly secreted during menstrual cycles and may offer several advantages over traditional biomarkers of ovarian reserve. Objective: To assess the relationship of anti-Mullerian hormone (AMH values, which are used to evaluate ovary reserves, with oocyte and embryo quality and with ART outcomes in patients undergoing intra-cytoplasmic sperm injection (ICSI. Materials and Methods: This cross sectional study was performed using 50 women undergoing ICSI in IVF center of Zeynep Kamil Women's and Children's Hospital, İstanbul, Turkey. All patients received the long protocol. Follicle-stimulating hormone, luteinizing hormone, estradiol, and AMH levels were measured and antral follicle counts were obtained on the 3rd day of menstruation. A cut-off value based on the number of oocytes was determined for AMH, and women were evaluated after being divided into two groups as bad responders and good responders, according to their AMH levels. Results: Twelve (27.3% women were in bad responders group and 32 (72.7% women were in good responders group. AMH measurements were statistically significantly different between the two groups (p<0.01. Based on this significance, the researchers used ROC analysis to estimate a cut-off point for AMH. The researchers detected the good responders with an AMH level 1.90 or above, with 87.50% sensitivity, 66.67% specificity, 87.50% positive prediction, and 66.67% negative prediction (AUC=0.777, p<0.01. Conclusion: Basal AMH levels can be used as an indicator to determine the ovarian response in women undergoing ICSI. AMH can be used to predict the number of mature oocytes that can be collected during treatment and the number of oocytes that can be fertilized. However, AMH is not a valuable tool to evaluate oocyte quality, the development of high-quality embryos, or pregnancy conception.

  16. Early postnatal methoxychlor exposure inhibits folliculogenesis and stimulates anti-Mullerian hormone production in the rat ovary.

    Science.gov (United States)

    Uzumcu, Mehmet; Kuhn, Peter E; Marano, Jason E; Armenti, AnnMarie E; Passantino, Lisa

    2006-12-01

    Methoxychlor [1,1,1-trichloro-2,2-bis(4-methoxyphenyl) ethane; MXC] is a chlorinated hydrocarbon pesticide commonly used in the United States as a replacement for DDT [1,1,1-trichloro-2,2-bis(p-chlorophenyl)ethane]. While MXC is a weak estrogenic compound, its more active, major metabolite [2,2-bis(p-hydroxyphenyl)-1,1,1-trichloroethane; HPTE] shows estrogenic, anti-estrogenic, or anti-androgenic properties depending on the receptor subtype with which it interacts. Anti-Mullerian hormone (AMH) is a paracrine factor that suppresses initial follicle recruitment in the ovary. Studies have shown the effects of exposure to MXC on adult ovarian morphology and function. However, the effect of exposure to MXC at an early postnatal stage on pre-pubertal follicular development and ovarian AMH production has not been studied. Around postnatal day (P) 4, most of the primordial follicular assembly in rats is complete, and a large number of primordial follicles transition into the primary follicle stage, a process that is inhibited by estrogen. The objective of this study was to examine the effect of early postnatal (P3-P10) MXC exposure on ovarian morphology and size, follicle number, and AMH production in the pre-pubertal (P20) rat ovary and to investigate the effect of HPTE on AMH production in immature rat granulosa cells in vitro. Female rats were injected (s.c.) daily with vehicle (control) or 1, 10, 50, 100, or 500 mg MXC/kg per day (referred to here as 1MXC, 10MXC, and so forth.) between P3 and P10. On P20, uterine and ovarian weights were determined, ovarian histology was examined, and follicles were counted and classified into primordial, primary, secondary, pre-antral, or antral stages using the two largest serial sections at the center of the ovary. Ovarian AMH production was examined using immunohistochemistry and western blot analysis. The effect of HPTE (0.5-25 microM) on AMH production in cultured immature rat granulosa cells was determined by western blot

  17. Evaluation of Ovarian Reserve by Measurement of the Serum Levels of Anti-Mullerian Hormone and Follicle-Stimulating Hormone in Intracytoplasmic Sperm Injection Cycles

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    Roshan Nikbakht

    2010-01-01

    Full Text Available Background: It is important to evaluate ovarian reserves prior to intracytoplasmic sperm injection(ICSI treatment. The aim of this study is to determine the accuracy of anti-mullerian hormone(AMH as a marker for ovarian reserve and to compare it with day-3 serum follicle-stimulatinghormone (FSH levels.Materials and Methods: In this analytic, corss-sectional study, sequential sampling was done on70 infertile women who underwent ICSI treatment at Imam Khomeini Hospital, Ahvaz, Iran.Initially, 5cc of venous blood was drawn from each patient to measure serum AMH and FSH levelson the day-3 cycle.Patients were divided into two subgroups according to the numbers of oocytes retrieved. Patientswere classified as good responders if there were four or more oocytes retrieved, whereas patientswith less than four oocytes were poor responders.Results: The basal AMH level correlated with the number of oocytes retrieved (linear Pearsoncorrelation coefficient=0.599, however the basal FSH level had a weakly reverse correlation(correlation coefficient = -0.11.AMH levels had a sensitivity of 85% and specificity of 61.5%, with a cutoff value equal to 2.3 ng/mlwhich was higher than FSH.Conclusion: AMH serum levels are good predictors of ovarian reserve in comparison with FSH.

  18. Anti-Mullerian hormone serum values and ovarian reserve: can it predict a decrease in fertility after ovarian stimulation by ART cycles?

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    Tito Silvio Patrelli

    Full Text Available BACKGROUND: A variety of indicators of potentially successful ovarian stimulation cycles are available, including biomarkers such as anti-Mullerian hormone. The aim of our study was to confirm the usefulness of serum anti-Mullerian hormone assay in predicting ovarian response and reproductive outcome in women eligible for ART cycles. MATERIALS: Forty-six women undergoing ART cycles at the Centre for Reproductive Medicine in Parma were recruited from March-to-June 2010. INCLUSION CRITERIA: age<42 years; body-mass-index = 20-25; regular menstrual cycles; basal serum FSH concentration <12 IU/L and basal serum estradiol concentration <70 pg/mL. The couples included in our study reported a variety of primary infertility causes. All women underwent FSH stimulation and pituitary suppression (GnRH-agonist/GnRH-antagonist protocols. Women were considered poor-responders if they had ≤ 3 oocytes; normal-responders 4-9 oocytes and high-responders ≥ 10 oocytes. Serum samples for the AMH assays were obtained on the first and last days of stimulation. A P value ≤ 0.05 was considered statistically significant. RESULT: FSH levels increased significantly when AMH levels decreased. The total dose of r-FSH administered to induce ovulation was not correlated to AMH. The number of follicles on the hCG, serum estradiol levels on the hCG-day, and the number of retrieved oocytes were significantly correlated to AMH. The number of fertilized oocytes was significantly correlated to the AMH levels. No significant correlation was found between obtained embryos or transferred embryos and AMH. Basal serum AMH levels were significantly higher than those measured on the hCG-day, which appeared significantly reduced. There was a significant correlation between AMH in normal responders and AMH in both high and poor responders. CONCLUSIONS: Our data confirm the clinical usefulness of AMH in ART-cycles to customize treatment protocols and suggest the necessity of verifying an

  19. Age-Specific Serum Anti-Mullerian Hormone and Follicle Stimulating Hormone Concentrations in Infertile Iranian Women

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    Alireza Raeissi

    2015-04-01

    Full Text Available Background: Anti-Müllerian hormone (AMH is secreted by the granulosa cells of growing follicles during the primary to large antral follicle stages. Abnormal levels of AMH and follicle stimulating hormone (FSH may indicate a woman’s diminished ability or inability to conceive. Our aim is to investigate the changes in serum AMH and FSH concentrations at different age groups and its correlation with ovarian reserves in infertile women. Materials and Methods: This cross-sectional study analyzed serum AMH and FSH levels from 197 infertile women and 176 healthy controls, whose mean ages were 19-47 years. Sample collection was performed by random sampling and analyzed with SPSS version 16 software. Results: There were significantly lower mean serum AMH levels among infertile women compared to the control group. The mean AMH serum levels from different ages of infertile and control group (fertile women decreased with increasing age. However, this reduction was greater in the infertile group. The mean FSH serum levels of infertile women were significantly higher than the control group. Mean serum FSH levels consistently increased with increasing age in infertile women; however mean luteinizing hormone (LH levels were not consistent. Conclusion: We have observed increased FSH levels and decreased AMH levels with increasing age in women from 19 to 47 years of age. Assessments of AMH and FSH levels in combination with female age can help in predicting ovarian reserve in infertile women.

  20. Anti-Mullerian hormone levels decline under hormonal suppression: a prospective analysis in fertile women after delivery

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    Ortner Iris

    2011-07-01

    Full Text Available Abstract Background AMH's reported stability during periods of hormonal change makes it a practical tool in assessing ovarian reserve. However, AMH declines with age and age-specific cut-offs remain to be established in women with proven fertility. This study aims to determine age-specific ranges of AMH in women with proven fertility. Methods Two hundred-ten fertile women, aged 18-40 years, were prospectively recruited for AMH measurements within 14 days after delivery and age stratified into 3 groups (18-30, 31-36 and 37-40 years. Eligibility required spontaneous conception within a maximal period of six months. Autoimmune diseases, chemotherapy, radiation, ovarian surgery and polycystic ovary syndrome precluded inclusion. Results 95% confidence intervals of AMH declined with advancing female age from 0.9-1.1 to 0.6-0.9 and 0.2-0.4 ng/mL (P Conclusions Like infertile populations, fertile women demonstrate declining AMH with advancing age. Uniformly lower levels than in infertile women suggest that AMH levels do not appear as stable under all hormonal influences as previously reported.

  1. The Correlations of Anti-Mullerian Hormone, Follicle-Stimulating Hormone and Antral Follicle Count in Different Age Groups of Infertile Women

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    Ludmila Barbakadze

    2015-02-01

    Full Text Available Background: The objective of our study was to identify the correlations between the tests currently used in ovarian reserve assessment: anti-Mullerian hormone (AMH, follicle stimulating hormone (FSH and antral follicle count (AFC and to distinguish the most reliable markers for ovarian reserve in order to select an adequate strategy for the initial stages of infertility treatment. Materials and Methods: In this prospective study, 112 infertile women were assessed. Subjects were divided into three age groups: group I <35 years (n=39, group II 35-40 years (n=31, and group III 41-46 years (n=42. AMH, FSH and AFC were determined on days 2-3 of the patients’ menstrual cycles. Results: There was a significantly elevated negative correlation between age and AMH level (rs =-0.67, p<0.0001 and AFC (rs =-0.55, p<0.0001. We observed a significantly positive correlation between age and FSH (rs =0.38, p<0.0001. AMH negatively correlated with FSH (rs =-0.48, p<0.0001 and positively with AFC (r=- 0.71, p=0.0001. There was a moderate negative relation between FSH and AFC (r=-0.41, p=0.0001 and moderate positive relation between age and FSH (rs =0.38, p<0.0001. The correlation analysis performed in separate groups showed that AMH and AFC showed a statistically significant positive correlation for group I (r=0.57, p<0.0001, group II (r=0.69, p<0.0001 and group III (r=0.47, p<0.002. A statistically significant correlation between FSH and AMH was detected only in groups I (r=-0.41, p<0.02 and II (r=-0.55, p<0.0001. A statistically significant correlation existed between FSH and AFC only in group III (r=-0.42, p<0.006, as well as between age and AFC only in group I (r=-0.35, p<0.03. Conclusion: Currently, AMH should be considered as the more reliable of the ovarian reserve assessments tests compared to FSH. There is a strong positive correlation between serum AMH level and AFC. The use of AMH combined with AFC may improve ovarian reserve evaluation.

  2. Anti-mullerian hormone cut-off values for predicting poor ovarian response to exogenous ovarian stimulation in in-vitro fertilization

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    Ruma Satwik

    2012-01-01

    Full Text Available Objectives: (a To establish the cut-off levels for anti-Mullerian hormone (AMH in a population of Indian women that would determine poor response. (b To determine which among the three ie.,: age, follicle stimulating hormone (FSH, or AMH, is the better determinant of ovarian reserve. Study Design: Prospective observational study. Setting: In vitro fertilization (IVF unit of a tertiary hospital. Materials and Methods: The inclusion criterion was all women who presented to the center for in-vitro fertilization/Intracytoplasmic sperm injection (IVF/ICSI. The exclusion criteria were age >45 years, major medical illnesses precluding IVF or pregnancy, FSH more than 20 IU/L, and failure to obtain consent. The interventions including baseline pelvic scan, day 2/3 FSH, luteinizing hormone (LH, estradiol estimations, and AMH measurement on any random day of cycle were done. Subjects underwent IVF according to long agonist or antagonist protocol regimen. Oocyte recovery was correlated with studied variables. The primary outcome measure was the number of oocytes aspirated (OCR. Three categories of ovarian response were defined: poor response, OCR ≤ 3; average response, OCR between 4 and 15; hyperresponse, OCR > 15. Results: Of the 198 patients enrolled, poor, average, and hyperresponse were observed in 23%, 63%, and 14% respectively. Correlation coefficient for AMH with ovarian response was r = 0.591. Area under the curve (AUCs for poor response for AMH, subject′s age, and FSH were 0.768, 0.624, and 0.635, respectively. The discriminatory level of AMH for prediction of absolute poor response was 2 pmoL/l, with 98% specificity and 20% sensitivity. Conclusions: AMH fares better than age and FSH in predicting the overall ovarian response and poor response, though it cannot be the absolute predictor of non-responder status. A level of 2 pmol/l is discriminatory for poor response.

  3. Up-regulation of SOX9 in sertoli cells from testiculopathic patients accounts for increasing anti-mullerian hormone expression via impaired androgen receptor signaling.

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    Kuo-Chung Lan

    Full Text Available BACKGROUND: Testosterone provokes Sertoli cell maturation and represses AMH production. In adult patients with Sertoli-cells-only syndrome (SCOS and androgen insensitivity syndrome (AIS, high level of AMH expression is detected in Sertoli cells due to defect of androgen/AR signaling. OBJECTIVE: We postulated that up-regulation of SOX9 due to impairment of androgen/AR signaling in Sertoli cells might explain why high level of anti-Mullerian hormone (AMH expression occur in these testiculopathic patients. METHODS: Biological research of testicular specimens from men with azoospermia or mouse. The serum hormone levels were studied in 23 men with obstructive azoospermia, 33 men with SCOS azoospermia and 21 volunteers with normal seminograms during a period of 4 years. Immunohistochemical staining and reverse-transcription PCR were used to examine the relationships among AR, SOX9 and AMH expression in adult human and mouse testes. The ability of AR to repress the expression of SOX9 and AMH was evaluated in vitro in TM4 Sertoli cells and C3H10T1/2 cells. RESULTS: SCOS specimens showed up-regulation of SOX9 and AMH proteins but down-regulation of AR proteins in Sertoli cells. The mRNA levels of AR were significantly lower and the SOX9, AMH mRNA levels higher in all SCOS patients compared to controls (P< 0.05. The testosterone levels in the SCOS patients were within the normal range, but most were below the median of the controls. Furthermore, our in vitro cell line experiments demonstrated that androgen/AR signaling suppressed the gene and protein levels of AMH via repression of SOX9. CONCLUSIONS: Our data show that the functional androgen/AR signaling to repress SOX9 and AMH expression is essential for Sertoli cell maturation. Impairment of androgen/AR signaling promotes SOX9-mediated AMH production, accounts for impairments of Sertoli cells in SCOS azoospermic patients.

  4. Follicular-fluid anti-Mullerian hormone (FF AMH is a plausible biochemical indicator of functional viability of oocyte in conventional in vitro fertilization (IVF cycles

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    Bindu N Mehta

    2013-01-01

    Full Text Available Context: Oocyte quality may be a governing factor in influencing in vitro fertilization (IVF outcomes. However, morphological evaluation of oocyte quality is difficult in conventional IVF cycles. Follicular-fluid (FF, the site for oocyte growth and development, has not yet been sufficiently explored to obtain a marker indicative of oocyte quality. Anti-Mullerian hormone (AMH is produced by granulosa cells of preantral and early-antral follicles and is released in FF. Aim: To investigate AMH as a biochemical indicator of functional viability/quality of oocyte produced in the FF micro-environmental milieu. Settings and Design: Prospective study involving 132 cycles of conventional IVF-embryo transfer (ET in infertile women. Subjects and Methods: AMH concentration was estimated in pooled FF on day of oocyte pickup. Cycles were sorted into low and high groups according to median (50 th centile values of measurement. Main outcome measure was oocyte viability, which included morphological assessment of oocyte quality, fertilization rate, clinical pregnancy, and implantation rates. Statistical Analysis: Graph-pad Prism 5 statistical package. Results: Low FF AMH group shows significantly higher percentage of top-quality oocytes (65.08 ± 24.88 vs. 50.18 ± 25.01%, P =0.0126, fertilization (83.65 ± 18.38 vs. 75.78 ± 21.02%, P =0.0171, clinical pregnancy (57.57 vs. 16.67%, P <0.0001, and embryo implantation rates (29.79 vs. 7.69%, P <0.0001 compared to high FF AMH group. FF AMH shares an inverse correlation with FF E2 (Pearson r = −0.43, r 2 = 0.18 and clinical pregnancy (Pearson r = −0.46, r 2 = 0.21. Threshold value of FF AMH for pregnancy is <1.750 ng/mg protein. Conclusion: FF AMH is a plausible biochemical indicator of functional viability of oocyte in conventional IVF cycles.

  5. Role of baseline antral follicle count and anti-Mullerian hormone in prediction of cumulative live birth in the first in vitro fertilisation cycle: a retrospective cohort analysis.

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    Hang Wun Raymond Li

    Full Text Available OBJECTIVE: This retrospective study determined for the first time the role of baseline antral follicle count (AFC and serum anti-Mullerian hormone (AMH level in the first in-vitro fertilisation (IVF cycle in predicting cumulative live birth from one stimulation cycle. METHODS: We studied 1,156 women (median age 35 years undergoing the first IVF cycle. Baseline AFC and AMH level on the day before ovarian stimulation were analysed. The main outcome measure was cumulative live birth in the fresh plus all the frozen embryo transfers after the same stimulation cycle. RESULTS: Serum AMH was significantly correlated with AFC. Both AMH and AFC showed significant correlation with age and ovarian response in the stimulated cycle and total number of transferrable embryos. Baseline AFC and serum AMH were significantly higher in subjects attaining a live birth than those who did not in the fresh stimulated cycle, as well as those attaining cumulative live birth. There was a significant trend of higher cumulative live birth rate in women with higher AMH or AFC. However, logistic regression revealed that both AMH and AFC were not significant predictors of cumulative live birth after adjusting for age and number of embryos available for transfer. Considering only one single predictor, the areas under the ROC curves for AMH (0.646, 95% CI 0.616-0.675 and age (0.648, 95% CI 0.618-0.677 were slightly higher than that for AFC (0.617, 95% CI 0.587-0.647 in predicting cumulative live birth. However, a model combining AMH (with or without AFC and age of the women only classified an addition of less than 2% of subjects correctly compared to the model with age alone. CONCLUSION: Baseline AFC and serum AMH have only modest predictive performance on the occurrence of cumulative live birth, and may not give additional value on top of the women's age.

  6. Comparing serum basal and follicular fluid levels of anti-Mullerian hormone as a predictor of in vitro fertilization outcomes in patients with and without polycystic ovary syndrome

    International Nuclear Information System (INIS)

    The prediction of in vitro fertilization (IVF) outcomes by anti-Mullerian hormone (AMH) measurement is getting increasing attention from clinicians. This study compares the relationship between serum or intrafollicular AMH levels and IVF outcomes in women with and without polycystic ovary syndrome (PCOS).This prospective study was carried out in two university-based fertility clinics. Serum samples were collected on cycle day 3 and follicular fluid (FF) was collected on the day of oocyte retrieval from 26 women with PCOS and 42 normo-ovulatory controls. AMH levels were measured in the samples using immunoenzymatic assay. The relationship between serum or FF AMH levels and IVF outcomes, including number of oocytes retrieved, oocyte maturation rate, fertilization rate, implantation rate, high quality grade embryo rate, and biochemical and clinical pregnancy rates were further assessed.Median serum basal AMH and FF AMH levels were significantly higher in the PCOS group as compared to controls, the values being 14.2 ng/mL vs. 3.2 ng/mL (P<.001) and 8.2 ng/g protein vs. 4.7 ng/g protein (P<.01), respectively. In both groups, serum basal AMH levels showed a positive correlation with number of oocytes retrieved (r=0.323; P=.037 in control vs. r=0.529; P=.005 in PCOS). In the control group, there was a positive relationship between serum basal AMH levels and percentage of matured oocytes (r = 0.331; P=.032) and implantation rate (r=0.305; P=.05).Serum basal, and not intrafollicular, AMH levels may be a good predictive factor for quantitative and qualitative IVF outcomes in normo-ovulatory, but not in PCOS patients (Author).

  7. The circadian variation in Anti-Mullerian hormone in patients with polycystic ovary syndrome differs significantly from normally ovulating women.

    Directory of Open Access Journals (Sweden)

    Leif Bungum

    Full Text Available OBJECTIVE: [corrected] To improve the biologic understanding of the Polycystic Ovarian Syndrome (PCOS condition by examining the circadian variation and relationship between Anti Müllerian Hormone (AMH, gonadotropins and ovarian steroids in PCOS patients compared to normally ovulating and menstruating women. By comparing the pattern of co-variation between AMH and Luteinizing Hormone, two compounds closely linked to hyperandrogenism and anovulation in PCOS, the involvement of the Hypothalamic-Pituitary-Ovarian axis in PCOS pathology could be elucidated. PATIENTS: Eight normal-weighted young, anovulatory PCOS-women as study group and ten normal menstruating and ovulating women as controls. INTERVENTIONS: Observational prospective study of the circadian variation in AMH, gonadotropins, sex steroids and androgens in a study and a control group. A circadian profile was performed in each study and control subject during a 24-h period by blood sampling every second hour, starting at 8:00 a.m. and continuing until 8:00 a.m. the following day. RESULTS: Significant differences in hormonal levels were found between the groups, with higher concentrations of AMH, LH and androgens in the PCOS group and lower amounts of FSH and progesterone. A distinct difference in the circadian variation pattern of AMH and LH between PCOS patients and normal controls was seen, with PCOS patients presenting a uniform pattern in serum levels of AMH and LH throughout the study period, without significant nadir late-night values as was seen in the control group. In PCOS women, a significant positive association between LH/ FSH and testosterone was found opposite to controls. MAIN OUTCOME MEASURES: Circadian variation in Anti-Müllerian Hormone, gonadotropins and ovarian steroids and the covariation between them. CONCLUSION: A significant difference in the circadian secretion of LH and AMH in PCOS women compared to normally ovulating women indicate an increased GnRH pulse

  8. Cut-Off Levels of Anti-Mullerian Hormone for The Prediction of Ovarian Response, In Vitro Fertilization Outcome and Ovarian Hyperstimulation Syndrome

    Directory of Open Access Journals (Sweden)

    Ali Salmassi

    2015-07-01

    Full Text Available Background: Evaluation of anti-mullerian hormone (AMH cut-off levels in assisted reproductive technology (ART as predictive factor for individualization of stimulation protocols and to avoid ovarian hyperstimulation syndrome (OHSS. Materials and Methods: In a retrospective study, 177 infertile patients were assessed for AMH in serum and follicular fluid (FF on the day of follicular puncture (FP, between 2012 and 2013 in Kiel, Germany. AMH levels and pregnancy rates were compared between low, moderate and high responders and cut-off levels of low and high responders. AMH cut-off levels in pathological cases were evaluated in analysis 1 (OHSS and in analysis 2 [polycystic ovarian syndrome, (PCOS] and compared in analysis 3 to normal endocrinological parameters. Results: AMH levels in FF were higher than in serum (P<0.001. AMH levels in serum and FF increased from low through moderate to high responders (P<0.001. Pregnancy rates were 14.7, 23.3 and 44.9% (P=0.009, respectively. AMH cut-off level for poor responders was 0.61 ng/ml in serum with a pregnancy rate of 13.8 and 37.1% for below and above of this level, respectively. For FF, it was 1.43 ng/ml. AMH levels in analysis 1 and 2 were significantly higher than in analysis 3 (P=0.001. AMH cut-off level for OHSS was 1.5 ng/ml in serum with OHSS rates of 80.8 and 19.2 % for above and below of the level, respectively. For FF, it was 2.7 ng/ml. PCOS patients had an AMH cut-off level of 3.9 ng/ml in serum and 6.8 ng/ml in FF, resulting in a PCOS rate of 100% above this level. Conclusion: AMH levels can help to assess ovarian response potential and guide ovarian stimulation while avoiding OHSS.

  9. Maternal menopause as a predictor of anti-Mullerian hormone level and antral follicle count in daughters during reproductive age

    DEFF Research Database (Denmark)

    Bentzen, J G; Forman, J L; Larsen, E C;

    2013-01-01

    this prospective cohort whose mothers' age at natural menopause was known. PARTICIPANTS, SETTING AND METHODS: Participants were recruited from female health care workers aged 20-40 years employed at Copenhagen University Hospital, Rigshospitalet, and were enrolled in the study between September 2008 and February...... AND GENERALIZABILITY: Information on 'age at maternal menopause' was obtained retrospectively and may be prone to recall bias and digit preference. The study population consisted of health care workers, which implies a potential selection bias. Finally, the cross-sectional nature of the data limits......STUDY QUESTION: Is the ovarian reserve in a woman at a given age associated with her mother's age at menopause? SUMMARY ANSWER: We demonstrated a significant, positive association between age at maternal menopause and serum anti-Müllerian hormone (AMH) levels and antral follicle count (AFC...

  10. Serum and follicular anti-Mullerian hormone levels in women with polycystic ovary syndrome (PCOS under metformin

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    Falbo Angela

    2010-07-01

    Full Text Available Abstract Background No data regarding metformin effects on follicular fluid anti-Müllerian hormone (AMH levels were to date available in literature. The aim of the present study was to evaluate in patients with polycystic ovary syndrome (PCOS whether metformin administration affects serum and follicular AMH levels, and whether this is related to ovarian response to the treatment. Methods Twenty young patients with PCOS who had received metformin were enrolled. Ten patients were anovulatory (Met-anov group, whereas the other 10 were ovulatory (Met-ov group but had failed to conceive. Further untreated PCOS (PCOS controls, n. 10 and healthy controls (non-PCOS controls, n. 10 who were scheduled for laparoscopic surgery were enrolled. In each subjects, clinical and biochemical evaluations were performed. AMH concentrations in blood and antral follicular fluid were assayed. Results In both Met-anov and Met-ov groups, and without difference between them, serum androgens and AMH, and indices of insulin resistance were significantly (p p Conclusions Metformin administration in patients with PCOS exerts a differential action on the ovarian AMH levels on the basis of ovulatory response. Changes in AMH levels in antral follicular fluid during metformin treatment could be involved in the local mechanisms mediating the ovulatory restoration.

  11. Prediction of reproductive outcomes according to different serum anti-Mullerian hormone levels in females undergoing intracystoplasmic sperm injection.

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    Santiago Brugo Olmedo

    Full Text Available BACKGROUND AND AIM OF THE STUDY: Serum anti-Müllerian hormone (AMH is a reliable marker of ovarian reserve, and it has been shown to be correlated with reproductive outcomes in grouped analyses. However, practical data is scarce for the physician and the patients to predict these outcomes in an individual couple according to serum AMH measured prior to assisted reproduction technology (ART procedures. STUDY DESIGN: To address this question, we performed an analytic observational study including 145 females undergoing intracytoplasmic sperm injection (ICSI in a single center. Results were analyzed according to serum AMH; subgroup analyses were performed by grouping patients according to patient's age and FSH levels. RESULTS: The risk of cycle cancellation decreased from 64% in patients with serum AMH ≤ 3 pmol/L (0.42 ng/mL to 21% with AMH ≥ 15 pmol/L (2.10 ng/mL. Cycle cancellation occurred in approximately two-thirds of the patients with AMH ≤ 3 pmol/L irrespective of the FSH level. However, with higher AMH values the risk of cycle cancellation decreased more significantly in patients with normal FSH. The rate of good response increased from almost null in patients with AMH ≤ 3 pmol/L to 61% in those with AMH ≥ 15 pmol/L. The positive correlation between good response and AMH was also significant, but with lower absolute rates, when patients were grouped according to their age or FSH levels. Pregnancy rate increased moderately, but significantly, from 31% with AMH ≤ 3 pmol/L to 35% with AMH ≥ 15 pmol/L. CONCLUSIONS: We provide estimates of reproductive outcomes according to individualized values of serum AMH, in general and in subgroups according to patient's age or serum FSH, which are helpful for the clinician and the couple in their decision making about starting an assisted reproductive treatment.

  12. Uso clínico do hormônio antimülleriano em ginecologia Clinical use for anti-mullerian hormone in gynecology

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    Gustavo Salata Romão

    2013-03-01

    Full Text Available O hormônio antimülleriano (AMH é uma glicoproteína produzida pelas células granulosas de folículos ovarianos primários, pré-antrais e pequenos folículos antrais e ultimamente sua aplicabilidade clínica tem sido demonstrada através de diversos estudos. A predição da resposta à estimulação ovariana para fertilização in vitro corresponde a sua mais frequente utilização na prática clínica, sendo rotineiramente realizada em muitos serviços para identificar subgrupos de mulheres suscetíveis a má resposta ou a Síndrome da Hiperestimulação Ovariana. Existem perspectivas de que o AMH possa ser aplicável na individualização do risco de injúria gonadal iatrogênica em mulheres portadoras de neoplasia que serão submetidas a quimioterapia. Também é provável que as dosagens de AMH sejam incluídas nos protocolos de investigação de amenorreias e oligomenorreias, uma vez que seus níveis encontram-se elevados em pacientes portadoras da Síndrome dos Ovários Policísticos, reduzidos em casos de falência ovariana prematura e normais em outras condições como a hiperprolactinemia e o hipogonadismo hipogonadotrófico. É possível que futuramente o AMH venha a ser utilizado na predição da idade de menopausa e do prognóstico reprodutivo da mulher, fornecendo bases sólidas ao aconselhamento conceptivo e contraceptivo.Anti-mullerian hormone (AMH is a glycoprotein produced by granulosa cells of primary, pre-antral and small antral ovarian follicles and its clinical applicability has been recently demonstrated by several studies. Prediction of the response to ovarian stimulation for in vitro fertilization corresponds to the most frequent utilization of AMH in clinical practice, being routinely assessed in many services to identify subgroups of women susceptible to a poor response or to Ovarian Hyperstimulation Syndrome. There are great perspectives that AMH may be applicable to the individual determination of risk for

  13. Changes in Anti-Mullerian Hormone (AMH) throughout the Life Span: A Population-Based Study of 1027 Healthy Males from Birth (Cord Blood) to the Age of 69 Years

    DEFF Research Database (Denmark)

    Aksglæde, Lise; Sørensen, K; Boas, M.;

    2010-01-01

    Context: Anti-Mullerian hormone (AMH), which is secreted by immature Sertoli cells, triggers the involution of the fetal Mullerian ducts. AMH is a testis-specific marker used for diagnosis in infants with ambiguous genitalia or bilateral cryptorchidism. Aim: The aim of the study was to describe...... the ontogeny of AMH secretion through life in healthy males. Setting:This was a population-based study of healthy volunteers. Participants: Participants included 1027 healthy males from birth (cord blood) to 69 yr. A subgroup was followed up longitudinally through the infantile minipuberty [(in cord blood......, and at 3 and 12 months), n = 55] and another group through puberty [(biannual measurements), n = 83]. Main Outcome Measures: Serum AMH was determined by a sensitive immunoassay. Serum testosterone, LH, and FSH were measured, and pubertal staging was performed in boys aged 6 to 20 yr (n = 616). Results...

  14. 抗苗勒氏管激素与多囊卵巢综合症治疗效果的相关性%Correlation of anti-mullerian hormone and the effect of polycystic ovary syndrome treatment

    Institute of Scientific and Technical Information of China (English)

    武雁; 宋晖; 田国华; 滑玮

    2016-01-01

    目的:研究多囊卵巢综合症治疗效果和抗苗勒氏管激素(Anti-Mullerian hormone,AMH)的相关性.方法:收集我院门诊2015年11月至2016年2月多囊卵巢综合征不孕症患者,排除其他因素后纳入140例.根据患者空腹血糖、空腹胰岛素和雄激素值分组:对照组:空腹血糖、空腹胰岛素和雄激素正常;糖代谢异常组:空腹血糖或/和空腹胰岛素升高,雄激素正常;高雄激素组:雄激素升高,空腹血糖或/和空腹胰岛素正常;糖代谢异常合并高雄激素组:空腹血糖或/和空腹胰岛素升高和雄激素升高的.给予达英-35或/和二甲双胍治疗后比较各组治疗前后和组间的抗苗勒氏管激素(Anti-Mullerian hormone,AMH)、空腹血糖、空腹胰岛素、胰岛素抵抗指数和雄激素变化.结果:糖代谢异常组,高雄激素组和糖代谢异常合并高雄激素组的AMH值,明显高于对照组(P<0.05).其中糖代谢异常合并高雄激素组的AMH值明显高于糖代谢异常组及高雄激素组(P<0.05).药物治疗后各组的AMH值比未治疗前均有明显下降(P<0.05).结论:AMH、糖代谢和雄激素三者可能具有互相影响、互相作用的关系.血清AMH水平为诊断和研究PCOS提供了一个重要的突破点.

  15. COMPARATIVE ANALYSIS OF SERUM ANTI-MULLERIAN HORMONE, SERUM FOLLICLE STIMULATING HORMONE AND SERUM ESTRADIOL IN THE PREDICTION OF OVARIAN RESERVE

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    Shuchita

    2013-05-01

    Full Text Available ABSTRACT:: A number off ovariian reserve ttestts are beiing used tto dettermiine oocytte reserve tto hellp prediictt iin viittro fferttiilliizattiion outtcome.. Thiis sttudy was underttaken tto ffiind iiff any correllattiion exiistts bettween llevells off Anttii--Mulllleriian (AMH,, ffolllliiclle sttiimullattiing hormone (FSH and esttradiioll (Ett,, wiitth tthe ovariian reserve.. Purposiive samplliing was underttaken.. IInfferttiilliitty pattiientts seekiing ttreattmentt att JJaiipur Ferttiilliitty and Mediicall Research Centtre,, a tterttiiary care uniitt off Mahattma Gandhii Mediicall Collllege and Hospiittall ffrom May--Dec2011 were iinclluded iin tthe sttudy.. IIn allll 105 pattiientts were sttudiied.. Serum llevells off hormones were dettermiined att day 3 and ovariian ffolllliiclle response was assessed on day off admiiniisttrattiion off HCG.. Resulltts revealled tthatt Serum AMH was siigniiffiicanttlly correllatted (r 0..302 p0..1 and Ett (r 0..999 p>0..1 were nott ffound tto have any such associiattiion wiitth ovariian reserve iin tthe sttudy.. KEY WORDS:: ovariian reserve,, AMH,, FSH,, esttradiioll,, IIVF.

  16. Ovarian response and cumulative live birth rate of women undergoing in-vitro fertilisation who had discordant anti-Mullerian hormone and antral follicle count measurements: a retrospective study.

    Directory of Open Access Journals (Sweden)

    Hang Wun Raymond Li

    Full Text Available OBJECTIVE: To evaluate ovarian response and cumulative live birth rate of women undergoing in-vitro fertilization (IVF treatment who had discordant baseline serum anti-Mullerian hormone (AMH level and antral follicle count (AFC. METHODS: This is a retrospective cohort study on 1,046 women undergoing the first IVF cycle in Queen Mary Hospital, Hong Kong. Subjects receiving standard IVF treatment with the GnRH agonist long protocol were classified according to their quartiles of baseline AMH and AFC measurements after GnRH agonist down-regulation and before commencing ovarian stimulation. The number of retrieved oocytes, ovarian sensitivity index (OSI and cumulative live-birth rate for each classification category were compared. RESULTS: Among our studied subjects, 32.2% were discordant in their AMH and AFC quartiles. Among them, those having higher AMH within the same AFC quartile had higher number of retrieved oocytes and cumulative live-birth rate. Subjects discordant in AMH and AFC had intermediate OSI which differed significantly compared to those concordant in AMH and AFC on either end. OSI of those discordant in AMH and AFC did not differ significantly whether either AMH or AFC quartile was higher than the other. CONCLUSIONS: When AMH and AFC are discordant, the ovarian responsiveness is intermediate between that when both are concordant on either end. Women having higher AMH within the same AFC quartile had higher number of retrieved oocytes and cumulative live-birth rate.

  17. 抗苗勒管激素在卵巢功能及相关疾病中的应用进展%Function of Anti-Mullerian Hormone in Ovary-related Diseases

    Institute of Scientific and Technical Information of China (English)

    刘云

    2016-01-01

    抗苗勒管激素(anti-Mullerian hormone,AMH)是睾丸支持细胞和卵巢颗粒细胞分泌的一种由二硫键连接两个相同亚基的二聚体糖蛋白,属于转化生长因子β超家族成员,具有调节两性生殖细胞发育的功能.在卵巢功能方面,AM能够调节卵泡发育,参与卵泡生长过程,还能早期评估卵巢储备情况,及早预测绝经年龄,更能早期发现卵巢疾病并评估疾病发展及预后,在辅助生殖技术中亦起到重要作用.随着检测技术的发展,AMH浓度的测量更加简便和精确,而且与其他生物学指标比较,AMH浓度相对稳定,所以AMH已成为近年来的研究热点之一.就近年来AMH与卵巢功能及相关疾病的关系综述如下.

  18. 抗苗勒氏管激素在多囊卵巢综合征诊断中的价值%The value of anti-mullerian hormone in the diagnosis of polycystic ovarian syndrome

    Institute of Scientific and Technical Information of China (English)

    刘俊芬; 程丽平

    2008-01-01

    目的:探讨抗苗勒氏管激素(Anti-Mullerian hormone,AMH)在多囊卵巢综合征(PCOS)中的诊断价值.方法:用酶联免疫吸附法(ELISA)测定56例PCOS患者血清AMH浓度,并且与30例正常妇女进行对照;以AMH 5.0ng/ml为界值,将其与PCOS的诊断标准进行对比,敏感度为89.29%,特异度为70.00%.结果:PCOS组与对照组血清AMH的浓度比较有显著性差异;在患病率较高的PCOS不孕诊断中,AMH检测是一项较好的指标.结论:在精确超声数据得不到的情况下,血清AMH浓度能够代替卵巢窦卵泡数目,是替代反映窦卵泡计数的理想指标.

  19. Follicle stimulating hormone and anti-Mullerian hormone per oocyte in predicting in vitro fertilization pregnancy in high responders: a cohort study.

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    Andrea Weghofer

    Full Text Available BACKGROUND: Follicle stimulating hormone (FSH and Anti-Müllerian hormone (AMH are utilized to differentiate between good and poor response to controlled ovarian hyperstimulation. Their respective roles in defining functional ovarian reserve remain, however, to be elucidated. To better understand those we investigated AMH and FSH per oocyte retrieved (AMHo and FSHo. METHODOLOGY/PRINCIPAL FINDINGS: Three-hundred and ninety-six women, undergoing first in vitro fertilization cycles, were retrospectively evaluated. Women with oocyte yields >75(th percentile for their age group were identified as high responders. In a series of logistic regression analyses, AMHo and FSHo levels were then evaluated as predictive factors for pregnancy potential in high responders. Patients presented with a mean age of 38.0±5.0 years, mean baseline FSH of 11.8±8.7 mIU/mL and mean AMH of 1.6±2.1 ng/mL. Those 88 women, who qualified as high responders, showed mean FSH of 9.7±6.5 mIU/mL, AMH of 3.1±3.1 ng/mL and oocyte yields of 15.8±7.1. Baseline FSH and AMH did not predict pregnancy in high responders. However, a statistically significant association between FSHo and pregnancy was observed in high responders, both after univariate regression (p = 0.02 and when adjusted for age, percentage of usable embryos, and number of embryos transferred (p = 0.03. Rate of useable embryos also significantly affected pregnancy outcome independently of FSHo (p = 0.01. AMHo was also associated with clinical pregnancy chances in high responders (p = 0.03 and remained significant when adjusted for usable embryos and number of embryos transferred (p = 0.04. CONCLUSIONS: AMHo and FSHo are predictive of pregnancy potential in high responders, but likely reflect different responsibilities in recruitment and maturation of growing follicle cohorts.

  20. Circadian variation in concentration of anti-Mullerian hormone in regularly menstruating females: relation to age, gonadotrophin and sex steroid levels

    DEFF Research Database (Denmark)

    Bungum, Leif; Jacobsson, Anna-Karin; Rosén, Fredrik;

    2011-01-01

    Anti-Müllerian hormone (AMH) is a promising marker of ovarian reserve. The aim of the study is to assess the circadian variation in AMH, and to evaluate its clinical relevance and biological aspects as an effect of age and other endocrine mechanisms involved in the regulation of AMH secretion....

  1. Body Mass Index Is Associated with Impaired Semen Characteristics and Reduced Levels of Anti-Mullerian Hormone across a Wide Weight Range.

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    Jorunn M Andersen

    Full Text Available There is still controversy as to how body mass index (BMI affects male reproduction. We investigated how BMI is associated with semen quality and reproductive hormones in 166 men, including 38 severely obese men. Standard semen analysis and sperm DNA integrity analysis were performed, and blood samples were analysed for reproductive hormones. Adjusted for age and time of abstinence, BMI was negatively associated with sperm concentration (B = -0.088, P = 0.009, total sperm count (B = -0.223, P = 0.001, progressive sperm motility (B = -0.675, P = 0.007, normal sperm morphology (B = -0.078, P = 0.001, and percentage of vital spermatozoa (B = -0.006, P = 0.027. A negative relationship was observed between BMI and total testosterone (B = -0.378, P < 0.001, sex hormone binding globulin (B = -0.572, P < 0.001, inhibin B (B = -3.120, P < 0.001 and anti-Müllerian hormone (AMH (B = -0.009, P < 0.001. Our findings suggest that high BMI is negatively associated with semen characteristics and serum levels of AMH.

  2. Cloning and expression of anti-mullerian hormone partial gene and preparation of polyclonal antibody%罗非鱼AMH基因的原核表达及多克隆抗体制备

    Institute of Scientific and Technical Information of China (English)

    丁炜东; 曹哲明; 曹丽萍

    2010-01-01

    抗苗勒氏管激素(anti-mullerian hormone,AMH),也称苗勒氏管抑制物质(mullerian inhibiting substance,MIS),为肽类生长因子,属于TGF-β生长和分化因子家族.为研究AMH 对奥利亚罗非鱼性腺发育的作用,应用DNAstar软件分析罗非鱼AMH基因的抗原性,选择抗原性较强的22~243氨基酸作为目的片段构建了AMH的原核表达载体并进行融合表达.首先利用RT-PCR方法从性腺中扩增出长约663 bp的目的序列AMH基因,克隆至T载体中,经酶切鉴定和序列测定分析确认序列的正确性后将此片段克隆到表达载体pGEX-5x-1中构建重组表达质粒pGEX-AMH,并在大肠杆菌BL21中获得了高表达,目的蛋白约占菌体总蛋白的38.7%.菌体经溶菌酶裂解,制备无细胞抽提液,GSTrap FF column柱层析后得到分子量为49 ku单一条带的目的蛋白.目的蛋白经FactorXa酶切裂解,GSTrap FF column过柱纯化后得到纯化的AMH蛋白,分子量为26 ku,浓度为2.6 mg/mL.以每只20μg的剂量4次免疫ICR小鼠,免疫小鼠可以检测到特异性针对AMH蛋白的血清抗体应答,免疫组抗体水平显著高于空白组(P<0.05),且加强免疫第5周后抗体效价为0.672±0.411,达到高峰值,血清效价为1∶2 000.试验结果表明表达产物具有免疫原性,可以刺激机体产生免疫应答.

  3. 抗苗勒管激素用于多囊卵巢综合征诊断的探讨%Study of Sersum Anti-mullerian Hormone as Marker for Diagnosis of Polycystic Ovary Syndrome

    Institute of Scientific and Technical Information of China (English)

    张芳; 王建辉

    2011-01-01

    目的:探讨抗苗勒管激素(AMH)在多囊卵巢综合征(PCOS)诊断的应用价值.方法:用电化学免疫发光法检测75例PCOS患者(研究组)及86例正常排卵育龄妇女(对照组)月经第2日内分泌水平,酶联免疫吸附法(ELISA)检测血清AMH,同时应用超声诊断仪行卵巢卵泡计数(FN).结果:①研究组与对照组血清AMH水平分别为(8.21±2.32) mg/L和(2.39±1.29) mg/L,研究组AMH水平明显高于对照组.②研究组AMH与FN、睾酮(T)、黄体生成激素(LH)、LH/FSH呈正相关(r分别为0.489,0.215,0.312和0.256,均P<0.05),而与卵泡刺激素(FSH)、雌二醇(E2)、泌乳素(PRL)、体质量指数(BMI)无线性相关性(r分别为0.12,0.19,0.10和0.16,均P>0.05).结论:AMH用于临床PCOS诊断有一定价值,且简便易行.%Objective:To explore whether anti-mullerian hormone (AMH) measurement in the serum could be a marker for diagnosis of polycystic ovary syndrome (PCOS). Methods: Serum AMH was measured with immunoassay in a cohort of 75 PCOS patients and 86 controls,and follicle number was estimated by ultrasound scanning. Results:Serum AMH levels were 4-fold higher in PCOS patients than in control group [ (8.21±2.32) mg/L vs. (2.39±1.29) mg/L] ,and were significantly related to the follicle number in the two groups. In PCOS patients, AMH concentrations correlated with the number of ovarian follicles,testosterone 、LH and LH/FSH(r= 0.489,0.215,0.312 and 0.256,P0.05).ConcIusions:The serum AMH level was a marker which may be used for diagnosis of the PCOS.

  4. A Tandem Duplicate of Anti-Mullerian Hormone with a Missense SNP on the Y Chromosome Is Essential for Male Sex Determination in Nile Tilapia, Oreochromis niloticus.

    Directory of Open Access Journals (Sweden)

    Minghui Li

    2015-11-01

    Full Text Available Variation in the TGF-β signaling pathway is emerging as an important mechanism by which gonadal sex determination is controlled in teleosts. Here we show that amhy, a Y-specific duplicate of the anti-Müllerian hormone (amh gene, induces male sex determination in Nile tilapia. amhy is a tandem duplicate located immediately downstream of amhΔ-y on the Y chromosome. The coding sequence of amhy was identical to the X-linked amh (amh except a missense SNP (C/T which changes an amino acid (Ser/Leu92 in the N-terminal region. amhy lacks 5608 bp of promoter sequence that is found in the X-linked amh homolog. The amhΔ-y contains several insertions and deletions in the promoter region, and even a 5 bp insertion in exonVI that results in a premature stop codon and thus a truncated protein product lacking the TGF-β binding domain. Both amhy and amhΔ-y expression is restricted to XY gonads from 5 days after hatching (dah onwards. CRISPR/Cas9 knockout of amhy in XY fish resulted in male to female sex reversal, while mutation of amhΔ-y alone could not. In contrast, overexpression of Amhy in XX fish, using a fosmid transgene that carries the amhy/amhΔ-y haplotype or a vector containing amhy ORF under the control of CMV promoter, resulted in female to male sex reversal, while overexpression of AmhΔ-y alone in XX fish could not. Knockout of the anti-Müllerian hormone receptor type II (amhrII in XY fish also resulted in 100% complete male to female sex reversal. Taken together, these results strongly suggest that the duplicated amhy with a missense SNP is the candidate sex determining gene and amhy/amhrII signal is essential for male sex determination in Nile tilapia. These findings highlight the conserved roles of TGF-β signaling pathway in fish sex determination.

  5. FOXL2 Is an Essential Activator of SF-1-Induced Transcriptional Regulation of Anti-Mullerian Hormone in Human Granulosa Cells.

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    Hanyong Jin

    Full Text Available Anti-Müllerian hormone (AMH is required for proper sexual differentiation by regulating the regression of the Müllerian ducts in males. Recent studies indicate that AMH could be an important factor for maintaining the ovarian reserve. However, the mechanisms of AMH regulation in the ovary are largely unknown. Here, we provide evidence that AMH is an ovarian target gene of steroidogenic factor-1 (SF-1, an orphan nuclear receptor required for proper follicle development. FOXL2 is an evolutionally conserved transcription factor, and its mutations cause blepharophimosis, ptosis, and epicanthus inversus syndrome (BPES, wherein affected females display eyelid defects and premature ovarian failure (POF. Notably, we found that functional FOXL2 is essential for SF-1-induced AMH regulation, via protein-protein interactions between FOXL2 and SF-1. A BPES-inducing mutant of FOXL2 (290-291delCA was unable to interact with SF-1 and failed to mediate the association between SF-1 and the AMH promoter. Therefore, this study identified a novel regulatory circuit for ovarian AMH production; specifically, through the coordinated interplay between FOXL2 and SF-1 that could control ovarian follicle development.

  6. Human blood contains both the uncleaved precursor of anti-Mullerian hormone and a complex of the NH2- and COOH-terminal peptides.

    Science.gov (United States)

    Pankhurst, Michael W; McLennan, Ian S

    2013-11-15

    Anti-Müllerian hormone (AMH) in blood is a marker of ovarian status in women and the presence of cryptic testes in babies. Despite this, the molecular form of AMH in blood has not been verified. AMH is synthesized as an inert proprotein precursor (proAMH), which can be cleaved to yield NH2-terminal (AMHN) and COOH-terminal (AMHC) fragments, that can complex noncovalently (AMHN,C). Developing males have 10-fold more AMH than young adults. We report here that human blood is a mixture of inactive proAMH and receptor-binding AMHN,C. The AMH in the blood of boys, men, and premenopausal women was immunoprecipitated using antibodies to the NH2- and COOH-terminal peptides. The precipitated proteins were then analyzed by Western blots, using recombinant proteins as markers. The glycosylation status of AMH was verified using deglycosylating enzymes. The NH2-terminal antibody precipitated a major protein that migrated alongside rhproAMH and was detected by anti-AMHN and anti-AMHC. This antibody also precipitated significant levels of AMHN and AMHC from all participants. Antibodies specific to AMHC precipitated rhAMHC but did not precipitate AMHC from human blood. Hence, all the AMHC in human blood appears to be bound to AMHN. Both AMHN and proAMH were glycosylated, independent of age and sex. In conclusion, boys and young adults have the same form of AMH, with a significant proportion being the inactive precursor. This raises the possibility that the endocrine functions of AMH are partly controlled by its cleavage in the target organ. The presence of proAMH in blood may confound the use of AMH for diagnosis.

  7. Allelic variant in the anti-Mullerian hormone gene leads to autosomal and temperature-dependent sex reversal in a selected Nile tilapia line.

    Directory of Open Access Journals (Sweden)

    Stephan Wessels

    Full Text Available Owing to the demand for sustainable sex-control protocols in aquaculture, research in tilapia sex determination is gaining momentum. The mutual influence of environmental and genetic factors hampers disentangling the complex sex determination mechanism in Nile tilapia (Oreochromis niloticus. Previous linkage analyses have demonstrated quantitative trait loci for the phenotypic sex on linkage groups 1, 3, and 23. Quantitative trait loci for temperature-dependent sex reversal similarly reside on linkage group 23. The anti-Müllerian hormone gene (amh, located in this genomic region, is important for sexual fate in higher vertebrates, and shows sexually dimorphic expression in Nile tilapia. Therefore this study aimed at detecting allelic variants and marker-sex associations in the amh gene. Sequencing identified six allelic variants. A significant effect on the phenotypic sex for SNP ss831884014 (p<0.0017 was found by stepwise logistic regression. The remaining variants were not significantly associated. Functional annotation of SNP ss831884014 revealed a non-synonymous amino acid substitution in the amh protein. Consequently, a fluorescence resonance energy transfer (FRET based genotyping assay was developed and validated with a representative sample of fish. A logistic linear model confirmed a highly significant effect of the treatment and genotype on the phenotypic sex, but not for the interaction term (treatment: p<0.0001; genotype: p<0.0025. An additive genetic model proved a linear allele substitution effect of 12% in individuals from controls and groups treated at high temperature, respectively. Moreover, the effect of the genotype on the male proportion was significantly higher in groups treated at high temperature, giving 31% more males on average of the three genotypes. In addition, the groups treated at high temperature showed a positive dominance deviation (+11.4% males. In summary, marker-assisted selection for amh variant ss831884014

  8. The effect of serum inhibin B and anti-mullerian hormone measurement for predicting ovarian reserve and treatment options in assisted reproduction%抑制素B和抗苗勒管激素预测卵巢反应性

    Institute of Scientific and Technical Information of China (English)

    吴日然; 叶云; 廖月婵; 杜静

    2007-01-01

    近年来应用抑制素B(inhibinB,INHB)、抗苗勒管激素(anti-mullerian hormone,AMH)水平等做为预测卵巢储备功能的指标。INHB是转化生长因子β超家族的成员,由卵巢中小窦卵泡颗粒细胞直接产生,主要生理作用是反馈性抑制垂体卵泡刺激素(FSH)分泌,其在年龄上的变化早于FSH水平,

  9. The correlation ofseminal anti -mullerian hormone and infertility semen parameters%精浆抗苗勒氏管激素与不育症精液参数的相关性研究

    Institute of Scientific and Technical Information of China (English)

    张志杰; 陈小均; 赵萌; 张岳阳; 段平川; 贾玉森

    2015-01-01

    Objectives:To explore the correlation of seminal anti -mullerian hormone (AMH)levels and male infertility semen parameters.Methods:56 patients in the male infertility clinic were selected as infertility and another 41 healthy male with reproductive history were selected as the control group.The seminal plasma AMH and semen parameters (sperm concentration,motility,viability and malformation rate)in both groups were detected. The serum sex hormone of infertility group was detected.Results:1 .Infertility group and control group had no sig-nificant difference in age and abstinence time.2.AMH was positively correlated (P <0.01 )with class A sperm rate,sperm motility and sperm viability,and correlation with morphological.The infertility group and the control group had no significant difference in seminal AMH value.3.Seminal AMH of infertile patients with normal semen analysis was significantly higher than in patients with weak sperm (P≤0.01 ),and had no significant difference in normal and abnormal morphologically.In infertile group,Seminal AMH had no significant correlation with sex hor-mones.Conclusions:The seminal AMH level is positively correlated with class A sperm rate,sperm motility and sperm viability.With the improvement of semen analysis results,seminal AMH is improved,regardless of the state of infertility.Seminal AMH can be used as indicators in clinical testing of infertility treatment effect.%目的:探讨精浆中抗苗勒氏管激素(AMH)水平与男性不育症精液参数的相关性。方法:选取门诊就诊的男性不育症患者56例为不育组,另设有生育史的健康男性41例作为正常对照组,两组均检测精浆中抗苗勒氏管激素(AMH)、精液参数(精子的密度、活率、活力及畸形率),不育组测定血清性激素6项。结果:1.不育组与正常对照组年龄、禁欲时间无明显差异;2.不育组及正常对照组精浆 AMH 与 a 级精子率、精子活力、精

  10. 盆腔炎性疾病患者血清AMH水平规律初探%Study on the level of anti-Mullerian hormone in patients with pelvic inflammatory disease

    Institute of Scientific and Technical Information of China (English)

    杨旭辉; 张杰; 黄志承; 梁嘉颖; 杨少芬; 朱照平; 何峰; 刘风华

    2015-01-01

    目的 调查不同程度盆腔炎性疾病(pelvic inflammatory disease,PID)患者血清抗苗勒管激素(anti-Mullerian hormone,AMH)水平并与正常生育期女性血清AMH水平比较,以探讨PID患者AMH的表达规律及临床意义.方法 选择健康女性67例为对照组,轻中度PID患者121例、重度PID患者34例为观察组,各组再按年龄分为5组:<25岁A组、26 ~ 30岁B组,31 ~35岁C组、36 ~ 40岁D组、>40岁E组.ELISA法检测血清AMH水平并分析各组不同年龄段的分布规律及表达差异.结果 轻中度PID患者血清AMH平均(4.48±0.36) ng/mL,与年龄呈负相关(r=-0.432,P<0.01),即AMH随年龄的增长不断下降;A组、B组均值与C组、D组、E组比较差异均有统计学意义(P<0.01),E组与A、B、C组比较差异也有统计学意义(P<0.01).重度PID患者血清AMH平均(2.12±0.26) ng/mL,与年龄呈负相关(r=-0.428,P<0.05),AMH随着年龄的增长下降幅度不大,但40岁后女性AMH骤降;E组与其余各组比较,差异均有统计学意义(P<0.01).对照组血清AMH平均(3.91±0.51) ng/mL,与年龄呈负相关(r=-0.742,P<0.01),即AMH随年龄的增长不断下降;E组与其余各组两两比较,差异均有统计学意义(P<0.01),A、B组与C、D、E组两两比较差异也有统计学意义(P<0.05).不同年龄组重度PID患者与轻中度PID患者、对照组血清AMH均值比较差异有统计学意义(P<0.05).结论 年龄是影响PID患者和正常生育期女性血清AMH水平的重要因素,重度PID患者与轻中度PID患者、正常女性血清AMH水平在不同年龄段均有差异;轻中度PID患者血清AMH水平可参照正常生育期女性的水平.

  11. Clinical study of anti-Mullerian hormone in prediction of ovarian aging%抗苗勒管激素与年龄的关系及其预测卵巢衰老的临床研究

    Institute of Scientific and Technical Information of China (English)

    高丽虹; 蔡文伟; 朱琴; 李永祥; 金倩燕; 张梅光; 蔡英如; 周芝婷; 吴晓杰

    2015-01-01

    目的:研究不同年龄阶段月经正常的生育期妇女抗苗勒管激素(AMH)水平的变化,了解AMH与年龄的关系及其在预测卵巢衰老中的价值。方法选择2012年12月至2014年7月在嘉兴市妇幼保健院行健康体检的月经正常的1423例生育期妇女,按年龄阶段分为:≤25岁、26~30岁、31~35岁、36~40岁、≥41岁,检测其血清AMH水平,并分析与年龄的关系。结果≤25岁、26~30岁、31~35岁、36~40岁、≥41岁妇女的血清AMH水平中位数分别为3.62、3.10、2.27、1.07、0.45μg/L;不同年龄阶段妇女血清AMH水平比较,差异有统计学意义(H=232.386,P<0.01)。血清AMH水平与年龄呈负相关(r=-0.374,P<0.01);血清AMH水平在25岁后即开始下降,36岁后下降明显。logAMH (即AMH的对数)的二次回归拟合最佳地反映了AMH与年龄的关系。结论检测生育期妇女血清AMH水平可早期预示卵巢功能的下降,可作为预警卵巢衰老的指标。%Objective To explore the serum anti-Mullerian hormone (AMH) level in women of childbearing age with normal menstrual cycles. Methods A total of 1 423 women with regular menstrual cycles were selected and divided into 5 groups according to their ages, i.e.≤25, 26-30, 31-35, 36-40,≥41 years. Their serum levels of AMH were measured, and the relationship between AMH and age was analyzed. Results The serum AMH levels of 5 groups according to ages (≤25, 26-30, 31-35, 36-40, ≥41 years) were 3.62, 3.10, 2.27, 1.07, 0.45μg/L, respectively. The comparison of serum AMH levels in different age groups had significant difference (P<0.01). Serum AMH level declined with increasing age,and dropped significantly after 36. The serum AMH level and age showed a negative correlation with significant difference (r=-0.374, P<0.01). Quadratic regression of logAMH proximally reflected the relationship between AMH and age. Conclusion AMH determination for women of

  12. 血清抗苗勒管激素诊断多囊卵巢综合征的临床价值%Clinical value of serum anti-mullerian hormone in diagnosis of polycystic ovarian syndrome

    Institute of Scientific and Technical Information of China (English)

    杨小岗; 王芳; 武佳敏; 董永生; 赵志刚; 武保乡; 武春燕

    2011-01-01

    Objective To explore the characteristic relationship between the anti-Mullerian hormone (AMH)and the PCOS hormone and use the ROC curve method to evaluate the diagnosis value of AMH for PCOS.Methods Used the enzyme-linked immunosorbent assay(ELISA)to detect the serum level of AHM,follicle-stimulating hormone(FSH),lunteinizing hormone (LH),testosterone(T),the estradiol(E2) and the LH/FSH value in 155 patients with PCOS,and carried on comparing with 134 women with normal menstrual cycle(control group).At the same time ,drawh up the ROC curve SPSS and statistical analyzed these datum.Results(1)The serum hormone levels(AMH 10.45 ng/ml,T 0.83 ng/ml.LH10.21 mIU /ml)of PCOS group were obviously higher thanthose of control group(AHM 5.14 ng/ml,T 0.77ng/ml,LH 5.04 mIU/ml)(P<0.01).The E2 level of PCOS group(68.2 pg/ml)was obviously lower than that of control group (116.05pg/ml)(P=0.000).LH/FSH ratio of the two groups had no remarkable differences(P=0.877).(2)The area under the ROC curve for PCOS diagnosed by AMH was 0.828.The standard error of area was 0.023 which has the remarkable significance(P=0.000);while the best dividing value of PCOS diagnosed by AMH was 6.865 ng/ml,the sensitivity was 75.48%,specificity was 73.63% and diagnostic effciency was medium .Conclusions Detecting serum AMH level is a novel and important method of PCOS diagnosis and research,but it is not suitable for taking the LH/FSH ratio as diagnosis indicator of PCOS.%目的 探讨抗苗勒管激素(AMH)与多囊卵巢综合征(PCOS)激素特征的关系及使用ROC曲线法评价AMH对PCOS的诊断价值.方法 用酶联免疫吸附法(ELISA)测定155例PCOS患者血清AMH、卵泡刺激素(FSH)、黄体生成素(LH)、睾酮(T)、雌二醇(E2)水平及LH/FSH值,并与134例月经周期正常妇女进行对照.用SPSS绘制ROC曲线及统计分析.结果 (1)PCOS组血清激素(中位数)AMH 10.45 ng/ml、T0.83 ng/ml、LH 10.21 mIU/ml水平,明显高于对照组(AMH 5.14 ng/ml、T0.77ng/ml、LH 5.03 mIU

  13. 抗苗勒管激素评价子宫肌瘤剔除术后卵巢功能的改变%The effect on ovarian function after myomectomy assessed by serum anti-Mullerian hormone levels

    Institute of Scientific and Technical Information of China (English)

    王海英; 梁洁玲; 张仁礼; 毕燕玲; 罗怀钦; 蒋洲梅; 闻安民

    2014-01-01

    Objective The aim of this prospective study was to evaluate the changes in the ovarian reverse after myomectomy based on serum anti-Mullerian Hormone (AMH) levels. Methods This is a prospective longitudinal observational study. Serum AMH levels were measured at the baseline and 2 day , 3months after myomectomy in 35 women aging from 36 to 45years.Follicle stimulate hormone(FSH) and luteal hormone(LH) were measured at the same time. 35 women of the same age with fibroid who did not undergo operation were selected as control group. Result (1)AMH level is (1.54 ± 0.95)ng/mL,(1.18 ± 0.77)ng/mL,(1.50 ± 0.58 )ng/mL at 0 day, 2 days and 3 months after operation. AMH level decreased significantly at 2 days after operation (P 0.05).(2) Significant differences in the serial change of AMH levels existed at each time point between myomectomy group and control group (P <0.05). No significant differences in FSH or LH levels existed at each time point. Conclusion AMH is may be superior to FSH or LH in evaluating the changing of ovarian reverse. The study suggests that myomectomy affect the ovarian function for up to 3 months post-operatively , and hemorrhage during and after operation may decrease serum AMH levels.%目的:通过检测新的卵巢功能指标抗苗勒管激素(AMH),评估子宫肌瘤剔除术对于年轻患者卵巢功能的影响。方法:前瞻性队列观察研究,选取36~45岁因子宫肌瘤行子宫肌瘤剔除术患者35例,于术前,术后2 d,术后3个月检测血清 AMH、促卵泡生成素(FSH)、促黄体生成激素(LH)水平,选取同年龄组未行手术的35例子宫肌瘤患者为对照组,比较两组间及各时间点各激素水平的变化差异。结果:(1)子宫肌瘤剔除组患者术前、术后2 d、术后3个月AMH水平分别为(1.54±0.95)ng/mL,(1.18±0.77)ng/mL,(1.50±0.58)ng/mL,术后2 d AMH 水平较术前显著降低(P<0.05),术后3个月AMH 水平逐

  14. Effect of testosterone on secretion of anti-Mullerian hormone in mouse ovarian granular cells cultured in vitro%睾酮对体外培养小鼠卵巢颗粒细胞分泌抗苗勒管激素的影响

    Institute of Scientific and Technical Information of China (English)

    张颖; 郑金丹; 刘丽丽

    2013-01-01

    Objective To study the effect of testosterone on secretion of anti-Mullerian hormone (AMH) in mouse ovarian granular cells cultured in vitro.Methods Primary mouse ovarian granular cells were cultured in vitro and treated with testosterone at different concentrations. Expression of AMH in mouse ovarian granular cells was detected by ELISA.Results ELISA showed that the AMH was expressed in mouse ovarian granular cells. Twenty-four hours treatment of primary mouse ovarian granular cells with testosterone at the concentrations of 10-8 mmol/L, 10-7 mmol/L and 10-6 mmol/L up-regulated their secretion of AMH in a concentration- and time-dependent manner (P<0.01).Conclusion Testosterone up-regulates the secretion of AMH in mouse ovarian granular cells in a concentration-dependent manner.%目的:探讨睾酮对体外培养的小鼠卵巢颗粒细胞抗苗勒管激素(anti-Mullerian hormone,AMH)分泌的影响。方法小鼠卵巢颗粒细胞原代培养,添加不同浓度睾酮,ELISA法检测颗粒细胞AMH的表达。结果 ELISA法证实颗粒细胞中有AMH表达,不同浓度(10-8 mmol/L、10-7 mmol/L、10-6 mmol/L)睾酮作用24 h可促进颗粒细胞分泌AMH,且随睾酮浓度的增加、作用时间延长,AMH分泌明显增加(P<0.01)。结论睾酮促进小鼠卵巢颗粒细胞分泌AMH,高浓度比低浓度作用更强。

  15. 抗苗勒管激素在子宫全切术后卵巢功能评价中的研究%The role of anti-Mullerian hormone on the evaluation of ovarian function after total hysterectomy

    Institute of Scientific and Technical Information of China (English)

    尹红燕; 刘玉珍; 吕秀萍; 张鹏; 孙小云

    2014-01-01

    Objective To investigate the value of anti-Mullerian hormone (AMH)in evaluating ovarian function after total hysterectomy and the influence of total hysterectomy on ovarian function. Methods This study was conducted at the affiliated hospital of Weifang Medical College between February and November 2013.Thirty patients aged 40 and 50 years who underwent laparoscopic total hysterectomy for benign uterine diseases were enrolled into the case group and unaffected women in a similar age range constituted the control group.Serum levels of AMH,follicle-stimulating hormone (FSH),luteinizing hormone (LH)and estradiol (E2 )were assayed on the 7th to 10th day of menstruation before operation,at 1st month and 6th month of operation,respectively.At the same time,ovarian volume (OV)and ovarian stromal artery resistance index (RI)were measured through transvaginal ultrasound. Results In the case group,there were significant differences between pre-operation and at 1st month of operation in AMH [(1.49 ±075)μg/L vs.(1.10 ± 0.48)μg/L],FSH [(9.45 ±4.05)U /L vs.(12.44 ±6.76)U /L],LH [(10.02 ±7.90)U /L vs.(13.97 ±7.28) U /L],E2 [(55.55 ±14.68)ng/L vs.(44.45 ±13.81)ng/L],OV [(6.63 ±2.37)cm vs.(3.57 ±1.03)cm,and FI [(0.59 ±0.20)vs.(0.73 ±0.08)].However,only AMH levels showed a statistical significance between pre-operation and at 6th month of operation [(0.72 ±0.59)μg/L].In the control group,Levels of AMH,FSH,LH and E2 levels,OV and RI at 1st and 6th month of operation were insignificantly difference compared with preoperative levels. There were significant differences at 1st month of operation between the case group and the control group in AMH [(1.47 ± 0.75)μg/L,FSH (9.56 ±3.61)U /L,LH (9.94 ±6.04)U /L,E2 (51.53 ±14.60)ng/L],OV [(6.71 ±2.36)cm, and RI (0.69 ±0.21)].Only AMH level was significant different between case group and control group [(1.46 ±0.83)μg/L]at 6th month of operation. Conclusion It appears that total hysterectomy causes more or less loss of

  16. Elderly men have low levels of anti-Mullerian hormone and inhibin B, but with high interpersonal variation: a cross-sectional study of the sertoli cell hormones in 615 community-dwelling men.

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    Yih Harng Chong

    Full Text Available The Sertoli cells of the testes secrete anti-Müllerian hormone (Müllerian inhibiting Substance, AMH and inhibin B (InhB. AMH triggers the degeneration of the uterine precursor in male embryos, whereas InhB is part of the gonadal-pituitary axis for the regulation of sperm production in adults. However, both hormones are also putative regulators of homeostasis, and age-related changes in these hormones may therefore be important to the health status of elderly men. The levels of AMH in elderly men are unknown, with limited information being available about age-related changes in InhB. We have therefore used ELISAs to measure Sertoli cell hormone levels in 3 cohorts of community-dwelling men in New Zealand. In total, 615 men were examined, 493 of which were aged 65 or older. Serum AMH and InhB levels inversely correlated with age in men older than 50 years (p<0.001 but not in the younger men. A minority of elderly men had undetectable levels of AMH and InhB. The variation in hormone levels between similarly aged men increased with the age of men. AMH and InhB partially correlated with each other as expected (r = 0.48, p<0.001. However, the ratio of the two Sertoli hormones varied significantly between men, with this variation increasing with age. Elderly men selected for the absence of cardiovascular disease had AMH levels similar to those of young men whereas their InhB levels did not differ from aged-matched controls. These data suggests that Sertoli cell number and function changes with age, but with the extent and nature of the changes varying between men.

  17. Relationship between anti-mullerian hormone in follicular fluid and telomere length in cumulus cells with oocytes at different mature stages%抗苗勒管激素及卵丘细胞端粒长度与卵母细胞成熟度的相关研究

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    王兴玲; 蔡鹏飞; 张文娟; 肖雅琳

    2016-01-01

    目的:探讨体外受精-胚胎移植(in vitro fertilization-embryo transfer,IVF-ET)中卵泡液内抗苗勒管激素(anti-mullerian hormone,AMH)水平及对应的卵丘细胞端粒长度与卵母细胞成熟度的关系.方法:收集52例行IVF-ET患者卵泡液及卵丘细胞,依据每位患者卵母细胞成熟度,将成熟卵泡液、未成熟卵泡液分别混合标记为成熟的卵泡液组(follicular fluids of mature oocyte,FFMⅡ)、未成熟的卵泡液组(follicular fluids of immature oocyte,FFMI).从卵母细胞周围机械剥离的部分卵丘颗粒细胞同样依据卵母细胞成熟度分组,标记为成熟卵母细胞的颗粒细胞组(cumulus cells of mature oocyte,CCMⅡ)、未成熟卵母细胞的颗粒细胞组(cumulus cells of immature oocyte stage,CCMI).应用酶联免疫法(enzyme-linked immunosorbent assay,ELISA)检测卵泡液中AMH,实时定量PCR测定卵丘细胞端粒长度.结果:未成熟卵泡液组中AMH水平高于成熟组[(6.02±1.34) vs.(5.66±1.42)],差异未达到统计学意义(P=0.13);未成熟组卵丘细胞端粒长度较成熟组的短[(3.50±1.08) vs.(4.40±1.24)],差异有统计学意义(=0.000).结论:未成熟的卵泡液组中AMH水平高于成熟组,卵泡液中AMH可能与卵母细胞成熟度有关,卵丘细胞端粒长度可以反映卵母细胞成熟度.

  18. The values of serum and follicular fuild anti-mullerian hormone levels of PCOS patients on predicting the outcomes of IVF/ICSI%血清及卵泡液抗苗勒管激素在多囊卵巢综合征患者体外受精促排卵治疗中的价值

    Institute of Scientific and Technical Information of China (English)

    陈雅; 叶碧绿; 郑九嘉; 林金菊; 赵军招; 张李雅; 杨旭

    2015-01-01

    目的:探讨长方案及拮抗剂方案的多囊卵巢综合征(polycystic ovary syndrome,PCOS)患者血清及卵泡液中抗苗勒管激素(anti-Mullerian hormone,AMH)水平对体外受精(IVF)结局的预测价值.方法:本前瞻性研究将59例行IVF治疗的PCOS患者随机分为口服避孕药后长方案(OC+ LP)30例和拮抗剂方案组29例,分别测定IVF治疗前一月经周期第2~5天(bAMH)、启动日(Gn日AMH)、取卵日(OPU日AMH)及卵泡液(FF AMH)的AMH水平,比较两组临床、实验室指标及AMH值,并分析AMH在促排卵过程中的变化.结果:OC+ LP组和拮抗剂组获卵数、成熟率、受精率、种植率及临床妊娠率等差异均无统计学意义(P>0.05),两组同一时间点AMH值比较差异均无统计学意义(P>0.05),无论是OC+LP组还是拮抗剂组,同一组内不同时间点AMH值比较显示,OPU日血清AMH水平均显著低于bAMH、Gn日AMH及FF AMH,差异具有统计学意义(P<0.05),而后三者两两相比,差异均无统计学意义(P>0.05).两组合并后按妊娠结局分临床妊娠组22例和非妊娠组20例,其年龄、获卵数、优质胚胎率、bAMH、Gn日AMH、OPU日AMH及FF AMH值差异均无统计学意义(P>0.05).结论:PCOS患者无论行长方案或拮抗剂方案,均不影响AMH对其卵泡发育的调控;血清及成熟卵泡液中AMH水平均不能预测PCOS患者IVF临床结局.

  19. 多囊卵巢综合征患者抗苗勒氏管激素与干细胞因子的相关性%Correlation between anti-mullerian hormone and stem cell factor in patients with polycystic ovary syndrome

    Institute of Scientific and Technical Information of China (English)

    王飞苗; 罗艳; 马会明; 吴昕; 胡蓉

    2012-01-01

    目的 研究多囊卵巢综合征(PCOS)患者抗苗勒氏管激素(AMH)和干细胞因子(SCF)的表达情况及二者的相关性.方法 选择年龄均小于35岁行体外受精与胚胎移植的PCOS患者102例,正常对照组90例,采用ELISA法检测血清和卵泡液中AMH和SCF的蛋白水平,分离并培养颗粒细胞;采用Real-time PCR检测PCOS患者颗粒细胞中AMH和SCF mRNA的相对含量,并对其进行相关性分析.结果 ①PCOS患者血清、卵泡液及颗粒细胞中AMH的相对表达较正常对照组明显升高,而SCF较对照组显著降低(P<0.01);②PCOS患者血清、卵泡液及颗粒细胞中AMH与SCF的表达均呈极为显著负相关(P<0.01).结论 多囊卵巢综合征患者血清、卵泡液及颗粒细胞中AMH与SCF的表达呈显著负相关.%To study the expressions of and correlation between anti-mullerian hormone (AMH ) and stem cell factor (SCF) in patients with polycystic ovary syndrome

  20. Serum Anti Mullerian Hormone (AMH) Tube Used in Infertility in Patients%血清抗缪勒氏管激素(AMH)在不孕不育症中的临床应用探讨

    Institute of Scientific and Technical Information of China (English)

    单咏梅; 庄严; 杨凡; 万海英

    2015-01-01

    目的:通过检测不孕不育症患者血清中抗缪勒氏管激素(anti-mul erian hormone,AMH),及卵泡刺激素(Fol icle-stimulating hormone,FSH)、黄体生成素(luteinizing hormone,LH)、雌二醇(Estradiol,E2)的血清表达水平,并进行比对、分析,探索AMH在不孕不育症中的临床应用价值。方法用化学发光法在ADVIA Centaur XP仪器上检测LH、FSH、E2,再用酶联免疫法对AMH进行测定,最后将这些指标用EXCEL软件进行线性回归分析。结果①根据厂商提供的AMH参考值可知,在FSH、LH、E2特别高的39例不孕不育育龄女性的标本中,38例都在该年龄段的参考范围内,只有1例超过参考范围,但是在不同年龄范围内低于中值年龄的30例患者中,有90.9%的患者AMH浓度远低于参考范围给出的中值浓度;②根据对LH、FSH、E2和AMH线性回归分析可知,LH、FSH、E2和AMH没有直接的线性关系;③LH/FSH呈一定程度的正相关。结论根据不同年龄段的育龄妇女的AMH浓度在该年龄段是否偏低或者异常增高来在一定程度上评估其生育能力,并通过进一步研究更为细分不同年龄段的妇女AMH表达情况,并建立合适的相应参考区间。 LH、FSH、E2和AMH没有太大的线性关系,这可能与LH、FSH、E2在月经周期期间波动很大、搜集的标本为不同年龄的、而AMH浓度不受月经周期影响、会受年龄影响有关。此外,有相关文章表明,经过年龄校正后LH/FSH的比值与AMH呈部分相关。而本试验做出LH/FSH呈一定程度的正相关,LH/FSH的比值是一个有用可靠评估卵巢储备功能的指标,并且可以和AMH共同应用于临床评估。因此AMH可以在一定程度应用于不孕不育症。%Objective Through the detection of anti-Mul erian hormone and fol icle stimulating hormone, luteinizing hormone, estradiol, comparison analysis, to explore the application value of AMH in the infertility.Methods The detection of LH, FSH, E2 in

  1. The Expression of Anti-Mullerian Hormone and Its Type Ⅱ Receptor Gene Polymorphisms in Patients with Polycystic Ovarian Syndrome%抗苗勒管激素及其Ⅱ型受体基因多态性在多囊卵巢综合征患者表达的研究

    Institute of Scientific and Technical Information of China (English)

    胡蓉; 姜银芝; 田春花; 田进石; 兰生学; 王飞苗; 贾韶彤; 张晓梅; 李彩艳; 杨海燕

    2012-01-01

    Objective:To investigate the gene polymorphism of the anti-mullerian hormone (AMH) and its type II receptor (AMHR II) in polycystic ovarian syndrome (PCOS), and to analyze the relationship of body mass index (BMI) and sex hormone levels in patients with different AMH gene types, so as to explore the possible mechanism of PCOS in gene level. Methods: Genotype distribution and allele frequencies of AMH and AMHR II were examined by polymerase chain reaction (PCR) and DNA forward and reverse sequencing in 94 PCOS patients and 94 normal women as control. The age, BMI and hormone levels of different AMH, AMHR II genotypes were compared. Results:①There was a significant difference of AMH genotype distribution in the two groups (P0.05); ②There were obvious difference of BMI, LH, T levels in PCOS patients with different AMH genotypes (P0.05). In the control group, there was no correlation between AMH genotype distribution and patient age, BMI and sex hormone levels( P >0.05). ③There were no correlations between the AMHR2 genotype distribution and patient age, BMI and sex hormone levels in either normal control or PCOS patients (P>0.05). Conclusions:AMH gene polymorphism may be associated with the pathogenesis of PCOS, also the G/T phenotype in AMH genotype may be the reason for obesity and hyperandrogenism in PCOS.%目的:研究抗苗勒管激素(AMH)及抗苗勒管激素Ⅱ型受体(AMHRⅡ)基因多态性在多囊卵巢综合征( PCOS)患者的表达情况;同时分析PCOS不同AMH基因型患者体重指数(BMI)、性激素水平之间是否存在差异,从基因水平探讨PCOS的发病机制.方法:采用聚合酶链反应(PCR)及DNA正、反向测序方法,检测94例PCOS患者(PCOS组)及94例正常排卵患者(对照组)AMH和AMHRⅡ基因型,同时将两组AMH、AMHRⅡ不同基因型患者的年龄、BMI及性激素水平分别进行比较.结果:①两组AMH基因型分布比较,差异有统计学意义(P<0.05),而AMH等位基因频率、AMHRⅡ基因

  2. 抗苗勒氏管激素在多囊卵巢综合征中的诊断价值初探%The Diagnostic Value of Serum Anti-Mullerian Hormone (AMH) Measurement in PCOS

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    李琳; 翁梅英; 陈晓莉; 陈亚肖; 钟俊敏; 杨冬梓

    2009-01-01

    Objective:To detect the serum anti-Müllerian hormone (AMH) level of patients with polycystic ovary syndrome (PCOS), and to explore the diagnostic value of serum AMH measurement in PCOS.Methods:47 patients with PCOS and 40 healthy females were enrolled from June 2006 to December 2006 in the Second Affiliated Hospital of Sun Yat-sen University. Serum AMH levels were measured by enzyme linked immunosorbent assay (ELISA). Results: The mean serum AMH level in PCOS group was significantly higher than control group (9.85±4.93 ng/ml vs 7.13±3.02ng/ml, P=0.002), which is similar in adult PCOS patients and controls (10.64±5.47 ng/ml vs 7.01 ± 2.43 ng/ml, P=0.005). No difference was found in serum AMH of adolescent PCOS patients and controls (8.95± 4.18 ng/ml vs 7.26± 3.58 ng/ml, P = 0.168). The areas under the ROC curve for AMH in all PCOS population, adults and adolescents were 0.664 (P=0.008), 0.714 (P = 0.015), and 0.614 (P = 0.208), respectively. The best compromise between specificity (70.0%) and sensitivity (61.7%) was obtained with a cut-off value of 8 ng/ml. Conclusions: Serum AMH level was elevated in patients with PCOS. Serum AMH measurement offers a relatively poor sensitivity of 61.7% and a specificity of 70% with a cut off value at 8 ng/ml, suggesting the diagnostic value of AMH was limited.%目的:研究多囊卵巢综合征(PCOS)患者的血清抗苗勒氏管激素(AMH)水平,并初步探讨其在PCOS中的诊断价值.方法:收集2006年6月至12月在我院妇产科就诊的47例PCOS患者(研究组)和年龄匹配的40例健康女性(对照组),应用酶联免疫吸附(ELISA)法检测血清AMH水平.结果:研究组的血清AMH水平明显高于对照组(9.85±4.93 ng/ml vs 7.13±3.02 ng/ml,P=0.002).血清AMH的曲线下面积在总体人群为0.664(P=0.008),以血清AMH 8 ng/ml为相对最佳的诊断界值,敏感度为61.7%,特异度为70.0%.结论:PCOS患者的血清AMH水平明显增高,血清AMH测定对于PCOS的诊断效能较低.

  3. 抗苗勒氏管激素检测对子宫切除术后患者卵巢功能的评价%Evaluation on ovarian function after hysterectomy by detection of serum anti-Mullerian hormone levels

    Institute of Scientific and Technical Information of China (English)

    王海英; 梁洁玲; 毕燕玲; 张仁礼; 罗怀钦; 蒋洲梅; 闻安民

    2014-01-01

    目的:探讨检测新的卵巢功能评价指标抗苗勒氏管激素(AMH)的价值,阐明子宫切除术对于年轻患者卵巢功能的影响。方法:选取36~45岁因子宫良性病变行全子宫切除术(子宫切除组)和同年龄段子宫肌瘤未行手术治疗(对照组)患者各35例,于不同时间点检测患者血清 AMH、促卵泡生成素(FSH)和促黄体生成激素(LH)水平,同时行阴道彩色多普勒超声监测卵巢动脉血流阻力指数(RI),分析不同时间点2组患者激素水平及卵巢动脉血流 RI的变化。结果:与术前比较,子宫切除组患者术后2 d及3个月 AMH 水平均明显降低(P<0.05或P<0.01)。与对照组比较,子宫切除组患者术后2 d及3个月血清 AMH 水平也明显降低(P<0.05)。子宫切除组患者术后1和3个月卵巢动脉血流 RI较术前和对照组明显增高(P<0.05)。子宫切除组和对照组患者不同时间点血清 FSH和 LH水平无明显变化(P>0.05)。结论:子宫切除术影响年轻患者卵巢功能,术后3个月 AMH水平下降,卵巢血流阻力增高。以血清 AMH 水平评估卵巢功能的改变优于传统的FSH和 LH指标。%Objective To evaluate the value of the new ovarian function marker serum anti-Mullerian hormone (AMH)and to clarify the effect of hysterectomy on the ovarian function of the younger women.Methods The serum AMH,follicle stimulating hormone (FSH)and luteinizing hormone (LH)levels in 35 women suffered uterus benign lesion aged 36-45 years and 35 women suffered hysteromyoma without operation were measured at different time.And the ovarian arterial blood flow resistance index (RI ) was measured by Doppler ultrasound. Results Compred with before operation, the serum AMH levels of the patients in hysterectomy group 2 d and 3 months after operation were significantly decreased (P0.05).Conclusion Hysterectomy can affect the ovarian function,and the serum AMH level

  4. Correlation between anti-Mullerian hormone and follicle-stimulating hormone receptor mRNA expression in granulosa cells in vitro.%体外颗粒细胞培养中抗苗勒管激素与卵泡刺激素受体mRNA表达的相关性

    Institute of Scientific and Technical Information of China (English)

    张迪; 桑丽英; 崔蓉; 苗竹林; 钟兴明; 韦相才

    2012-01-01

    目的 体外培养小鼠原代颗粒细胞培养液中,加入不同浓度的抗苗勒管激素(AMH),比较各组间小鼠颗粒细胞上卵泡刺激素受体(FSHR)mRNA的表达情况,探讨体外培养颗粒细胞中AMH与FSHRmRNA表达的关系.方法 选取4~5周的昆明雌性小鼠,腹腔注射孕马血清促性腺激素,48~54h后颈椎脱臼法处死小鼠,取双侧卵巢,采用机械分离法释放颗粒细胞,胰蛋白酶消化细胞,低速离心分离细胞,用含15%胎牛血清的DMEM/F12培养基置于37℃、5%CO2培养箱中培养.显微镜观察细胞形态,流式细胞术分离细胞,并用免疫荧光法对所培养的颗粒细胞进行鉴定.同时在体外培养的小鼠原代颗粒细胞培养液中加入不同浓度(5、10、20、30、40ng/mL)的AMH,测定每组培养基中AMH的终浓度,并用荧光定量PCR技术检测每组颗粒细胞中FSHR mRNA 的表达.结果 分离培养的颗粒细胞纯度大于80%;随AMH浓度加大,小鼠颗粒细胞中FSHR mRNA的表达逐渐降低,当AMH浓度为 5 和10ng/mL时,与对照组比较差异有统计学意义(P<0.05),且5 ng/mL浓度组颗粒细胞上FSHR mRNA的表达量最高.结论 用机械分离加胰酶消化加低速离心能得到纯度较高活性很好的颗粒细胞,用FSHR鉴定颗粒细胞是一种简便易行的方法,AMH浓度与颗粒细胞上FSHRmRNA表达呈一定负相关,表明AMH有可能通过抑制FSHR表达降低FSH敏感性,从而参与卵巢早衰的病理生理过程.%Objective To investigate the correlation between anti - Mullerian hormone (AMH) and follicle -stimulating hormone receptor (FSHR) mRNA expression in the cultured granulosa cells in vitro. Methods Kunming female mice were peritoneally injected with pregnant mare serum gonadotropin (PMSG) and sacrificed 48 to 54 hours after injection for collection of ovarian. Granulosa cells were isolated by mechanical method, trypsin digestion and low - speed centrifugation. Granulosa cells were diluted and incubated in

  5. Effect of follicle stimulating hormone on the secretion of anti-mullerian hormone in the granulosa cells in patients with polycystic ovarian syndrome%卵泡刺激素对多囊卵巢综合征患者卵巢颗粒细胞分泌抗苗勒管激素的影响

    Institute of Scientific and Technical Information of China (English)

    李轶; 梁晓燕

    2012-01-01

    目的 研究卵泡刺激素(FSH)对多囊卵巢综合征(PCOS)患者卵巢颗粒细胞分泌抗苗勒管激素(AMH)的影响.方法 选择2008年8月至2009年12月于中山大学附属第六医院生殖中心就诊的33例PCOS患者,从其直径为8~10 mm的窦卵泡中分离颗粒细胞,并分为以下3组进行培养:未经FSH刺激的颗粒细胞(未刺激组,n=12);外源性FSH刺激的颗粒细胞(体外刺激组,n=12),此组颗粒细胞来源同未刺激组;对患者行FSH促排卵治疗,采集FSH体内刺激的颗粒细胞(体内刺激组,n=21).分别采用ELISA法和实时荧光定量PCR技术检测培养液中颗粒细胞分泌的AMH水平,及AHM mRNA表达水平;构建AMH荧光报告载体,检测卵巢颗粒细胞中AMH启动子的活性.结果 培养液中颗粒细胞分泌的AMH水平,未刺激组为(11.4±4.0)μg/L,体外刺激组为(7.9±1.1)μg/L,体内刺激组为(5.6±1.7)μg/L,体内、体外刺激组分别与未刺激组比较,差异均有统计学意义(P均<0.05).AMH mRNA表达水平未刺激组为2.5±1.2,体外刺激组为1.5±0.5,体内刺激组为1.1±0.7,未刺激组高于体内、外刺激组,分别比较,差异均有统计学意义(P均<0.05).未刺激组颗粒细胞中AMH启动子的活性为11.5±2.3,体外刺激组为8.7±2.4,体内刺激组为6.8±2.4,未刺激组高于体内、外刺激组,分别比较,差异均有统计学意义(P均<0.05).结论 FSH可能通过抑制PCOS患者卵巢颗粒细胞中AMH的启动子活性及其mRNA表达,抑制AHM的过度分泌,促进卵泡的生长发育.%Objective To study the effect of follicle stimulating hormone(FSH)on the secretion of anti-mullerian hormone(AMH)in granulosa cells in patients with polycystic ovarian syndrome(PCOS).Methods From Aug.2008 to Dec.2009,33 patients with PCOS in Reproductive Medicine Research Center,Sixth Affiliated Hospital of Sun Yat-sen University were collected from 8-10 mm antral follicles and classified into the following three groups: FSH

  6. Anti-Mullerian Hormone: Molecular Mechanism of Action

    NARCIS (Netherlands)

    J.A. Visser (Jenny)

    1998-01-01

    textabstractDuring early fetal development. the primitive urogenital system is bipotential and can develop into a male or female direction, depending on the chromosomal sex of the fetus. In this chapter, regulatory factors of sex determination and differentiation are described. The roles of two test

  7. Association between Gene Polymorphism of Anti-Mullerian Hormone and Its Type Ⅱ Receptor and Ovarian Reserve%抗苗勒管激素及其Ⅱ型受体基因多态性与卵巢储备功能的关系

    Institute of Scientific and Technical Information of China (English)

    田春花; 胡蓉; 贾韶彤; 兰生学; 田进石; 王飞苗; 李彩艳; 杨海燕; 姜银芝

    2012-01-01

    Objective To investigate gene polymorphism of the anti-Mullerian hormone (AMH) and its type JJ receptor (AMHRTJ)in polycystic ovary syndrome (PCOS) and premature ovarian fail ure ( POF). Methods Polymerase chain reaction (PCR)and DNA sequencing were used to detect the distribution of AMH and AMHR JJ genotype and allele frequency in 94 cases of PCOS,94 normal women and 32 cases of POF. The correlation between AMH and AMHR JJ genotypes with age, body mass index(BMI)and hormone levels was analyzed. Results ? There was significant difference in AMH and AMHR JJ genotypes ,and AMH allele frequency among three groups of subjects(P 0. 05) ;? In PCOS patients, AMH genotype was correlated with BMI,LH,T levels(P0. 05);? In POF patients, AMHR H genotypes were associated with BMI,T and E2(P0. 05). Conclusion AMH and AMHRJJ gene polymorphism may be associated with PCOS,POF.%目的 探讨抗苗勒管激素(AMH)及其Ⅱ型受体(AMHRⅡ)基因多态性与卵巢储备功能的相关性.方法 采用聚合酶链反应(PCR)及DNA测序方法,检测94例多囊卵巢综合征(PCOS)患者、94例正常排卵女性及32例卵巢功能早衰(POF)患者AMH和AMHRⅡ基因型及等位基因频率的分布情况,分析患者AMH、AMHRⅡ基因型不同与其年龄、体重指数(BMI)及激素水平的相关性.结果 ① 3组研究对象AMH、AMHRⅡ基因型及AMH等位基因频率分布存在差异(均P<0.05),而AMHRⅡ等位基因频率分布无差异(均P>0.05);②PCOS患者AMH基因型与BMI、LH、T水平相关(均P<0.05),而正常对照组和POF患者AMH基因型与年龄、BMI、FSH、LH、T、E2、PRL水平无关(均P>0.05);③POF患者AMHR基因型与BMI、T及E2水平相关(均P<0.05),而正常组和PCOS患者AMHRⅡ基因型分布与其年龄、BMI、FSH、LH、T、E2、PRL水平均无关(均P>0.05).结论 AMH及AMHRⅡ基因的多态性可能与卵巢储备功能异常有关.

  8. 不孕不育患者抗子宫内膜抗体、抗精子抗体与抗苗勒管激素的检测价值%Detective value of anti endometrial antibody,antisperm antibody and anti-mullerian hormone in patients with infertility

    Institute of Scientific and Technical Information of China (English)

    王军

    2015-01-01

    目的:探讨不孕不育患者抗子宫内膜抗体(EMAb)、抗精子抗体(AsAb)与抗苗勒管激素(AMH)的检测价值。方法选取2011年5月~2014年5月我院就诊的不孕不育夫妇80对,另选择同期健康及近3年内有生育史夫妇60对作为对照组。采用酶联免疫吸附试验测定比较两组患者EMAb、AsAb和AMH的情况。结果不孕不育组血清EMAb、AsAb阳性率和AMH水平均明显高于对照组,差异有统计学意义(P<0.05);不孕不育患者联合检测EMAb、AsAb阳性率明显高于单独检测EMAb、AsAb,差异有统计学意义(P<0.05)。不孕不育组精液异常患者AsAb阳性率明显高于精液正常者,差异有统计学意义(P<0.05)。结论 AsAb、EMAb和AMH与不孕不育的发生密切相关,联合检测EMAb、AsAb在不孕不育的诊断及治疗监测方面具有重要的价值。%Objective To investigate the detective value of anti endometrial antibody(EMAb),antisperm antibody(AsAb)and anti-mullerian hormone(AMH)in patients with infertility.Methods80 couples of infertility who were treated in our hospital from May 2011 to May 2014 were selected,and 60 healthy couples with fertility history in the last 3 years were selected as the control group.EMAb,AsAb and AMH in the two groups were determined by enzyme-linked immunosorbent assay and compared. Results The positive rates of serum EMAb,AsAb and level of serum AMH in infertility group were significantly higher than those in the control group,the difference was statistically significant(P<0.05);and the positive rate of combined detection of EMAb and AsAb in patients with infertility was significantly higher than that of single EMAb or AsAb,the difference was statistically significant(P<0.05).The positive rate of AsAb in infertile group with abnormal sperm was significantly higher than that with normal sperm, and the difference was statistically significant(P<0.05).ConclusionAsAb,EMAb and AMH are closely

  9. Predictive Value of Anti-mullerian Hormone on Ovary Response in PCOS Patients with Clomiphene Citrate (CC)-resistant after Laparoscopic Ovarian Drilling%抗苗勒管激素对氯米芬抵抗的PCOS妇女腹腔镜术后卵巢反应性预测价值

    Institute of Scientific and Technical Information of China (English)

    韦成厚; 沈宏伟; 朱国平

    2012-01-01

    [Objective] To investigate the predictive diagnostic value of preoperation serum anti-mullerian hormone (AMH) level measurement on ovary ovulation response after laparoscopic ovarian drilling (LOD) in PCOS patients with clomiphene citrate (CC)-resistant. [Methods] 54 PCOS patients with clomiphene citrate(CC)-resistant were enrolled LOD. Serum AMH levels were measured by enzyme linked immunosorbent assay (ELISA) , Follicle growth and ovulation situation were supervised by vaginial ultrasound and urine LH peak. Using ROC (receiver operating characteristic) curve analysis monitoring data. [Results] ROC curve analysis indicated that AMH had moderate diagnostic value for postoperation high and poor ovary response. Theirs AUC (area under the curve, AUC) were 0.792 and 0.846 (95%CI: 0.673-0.911 and 0.696-0.996; P = 0.000 and P = 0.002). Serum AMH at 7.4 ng/mL and 4.3 ng/ mL were optium cutoff value for predictive high and poor ovary response, respectively. Sensitivity were 73.1% and 87.5% , respectively. Specificity were 71.4% and 66.7%, respectively. Positive predictive value were 71.9% and 72.4%, respectively. Negative predictive values were 72.6% and 84.2%, respectively. [Conclusions] The preoperation measurement of serum basal AMH level can predictive the high and poor ovary response after LOD in PCOS patients with clomiphene citrate (CC)-resistant, and act as a single valued predictive index in estimating LOD effectiveness.%[目的]探讨测定术前血清抗苗勒管激素(AMH)水平对氯米芬(CC)抵抗的多囊卵巢综合征(PCOS)妇女腹腔镜术后卵巢反应性预测价值.[方法]应用酶联免疫吸附(ELISA)法检测腹腔镜卵巢打孔术(LOD)54例氯米芬抵抗的PCOS患者术前血清基础AMH水平,术后用B超和尿LH峰监测卵巢卵泡发育及排卵情况,并用ROC曲线分析监测结果.[结果]ROC曲线分析表明,AMH对术后卵巢高和低反应性均为中等诊断价值,其AUC分别是0.792和0.846(95%CI:0.673-0.911和0.696-0.996;P

  10. 腹腔镜下次全子宫与全子宫切除、肌瘤剔除术对子宫肌瘤患者卵巢储备功能的影响%Effects of three surgical modalities on ovarian reserve by assessing the serum level of anti-Mullerian hormone for uterine fibroids

    Institute of Scientific and Technical Information of China (English)

    袁华; 赵绍杰; 龚华; 梁喜; 王沂峰

    2015-01-01

    To compare the effects of laparoscopic total or subtotal hysterectomy and myomectomy on ovarian reserve by measuring the serum level of anti-Mullerian hormone (AMH).A total of 96 patients with uterine fibroids underwent total laparoscopic hysterectomy (TLH group,n =32),laparoscopic subtotal hysterectomy (LSH group,n =31) and laparoscopic myomectomy (LM group,n =33).Changes in ovarian reserve were examined by measuring the level of AMH pre-operation and at 1 and 4 months post-operation.No significant differences existed in AMH in LM group[(1.42 ±0.65),(1.31 ±0.53) & (1.33 ±0.61) μg/L,P > 0.05].The levels of AMH in both groups were significantly lower than those at pre-operation [(1.17 ± 0.11),(1.01 ±0.10),(0.48±0.54) & (1.18±0.93),(0.45 ±0.39),(0.14±0.0) μg/L,P<0.001].The mean percentage decrease in AMH was lower in TLH group than that in LSH group at 4-month follow-up [(-37.4 ± 37.6) % vs.(-27.0 ± 23.0) %,P =0.017].The results showed that LM had no effect on ovarian reserve.Both LSH and TLH had adverse effects on ovarian reserve and TLH was more marked.%对行腹腔镜子宫肌瘤剔除术的33例(剔除组)、腹腔镜次全子宫切除术的31例(次全切组)和腹腔镜全子宫切除术的32例(全切组),于术前、术后1和4个月行血清抗苗勒管激素(AMH)水平测定.剔除组手术前后血清AMH水平比较差异无统计学意义[(1.42 ±0.65)、(1.31±0.53)和(1.33±0.61) μg/L,P>0.05];次全切组和全切组术后血清AMH水平均较术前显著下降[(1.17±0.11)、(1.01±0.10)、(0.48 ±0.54)和(1.18 ±0.93)、(0.45-0.39)、(0.14 ±0.00) μg/L,均P<0.001];全切组术后4个月血清AMH水平下降百分率高于次全切组[(-37.4±37.6)%比(-27.0±23.0)%,P=0.017].提示肌瘤剔除术对卵巢储备无显著影响,全子宫切除术的影响最大.

  11. 腹腔镜下卵巢良性囊肿剥除术对患者血清抗苗勒管激素水平的影响%Effect of Laparoscopic Cystectomy on Serum Anti-Mullerian Hormone Level in Patients with Diverse Benign Ovarian Cysts

    Institute of Scientific and Technical Information of China (English)

    奚晓雪; 杨晨; 戴建荣

    2015-01-01

    Objective To evaluate the effect of laparoscopic cystectomy on ovarian reserve in patients with diverse benign ovarian cysts by investigating the serum anti-Mullerian hormone (AMH) level before and after operation.Methods A total of 46 patients who underwent laparoscopic cystectomy due to unilateral ovarian cyst from April 2014 to April 2015 were recruited in this retrospective analysis. All patients were divided into endometrioma group and nonendometrioma group by pathological types. The surgery hemostatic method was bipolar electrocoagulation. 25 females in the same age were selected as the normal control group (NCG). Serum AMH levels were measured before operation and at 3 days, and 6 months follow-up visit afterwards.Results There were no signiifcant differences between nonendometrioma group and NCG in terms of serum AMH levels before operation. Serum AMH level was found to be lower in endometrioma group than that in nonendometrioma group and NCG before operation. Serum AMH level was found to be lower in both nonendometrioma group and endometrioma group after operation, AMH more markedly declined in endometrioma group at postoperative 3 days. At 6 months postoperatively, AMH levels were found to be similar between two groups.Conclusion Laparoscopic cystectomy could impair the ovarian reserve to some extent in patients with ovarian cysts in the immediate future and the effects could be related with the pathological patterns. The influence seems to be more obvious in patients with endometrioma.%目的:通过检测手术前后卵巢良性囊肿患者血清抗苗勒管激素(AMH)水平的变化,探讨腹腔镜下囊肿剔除术对卵巢储备功能的影响。方法选取2014年4月—2015年4月因单侧良性卵巢囊肿行腹腔镜下卵巢囊肿剔除术的46例患者,根据术后病理将患者分为巧囊组(n=23)、非巧囊组(n=23),术中创面止血采用双极电凝法。同时选取25例正常育龄妇女为对照组。于术前2 d

  12. 促性腺激素释放激素激动剂降调节对血清抗苗勒管激素的影响%Influence of Gonadotropin-releasing Hormone Agonist on Serum Anti-Mullerian Hormone Level

    Institute of Scientific and Technical Information of China (English)

    叶云; 吴日然; 林晓丽; 廖月婵; 杜彦; 吴嘉齐; 林秀峰; 杜静

    2013-01-01

    [Objective] To investigate whether the influence of two forms (long-acting / short-acting) of gonadotrophin-releasing hormone agonist (GnRH-a) on anti-Mullerian hormone (AMH) is the same.[Methods] The study included 869 cycles that underwent in vitro fertilization (IVF) /intracytoplasmic sperm injection (ICSI) from February 2012 to September 2012.Patients who met the inclusion criteria were randomly divided into two groups:long-acting group (using long-acting GnRH-a,Diphereline(R)) which including 128 women with 128 cycles,and short-acting group (useing short-acting GnRH-a,Diphereline(R)) which including 116 women with 116 cycles.Serum AMH levels were measured at baseline and Day 14 following GnRH-a.[Results] ① After down-regulation in long-acting group,baseline serum AMH slightly reduced,P > 0.05; In short-acting group,serum AMH fell from (2.92± 2.12) μg/L to (2.29 ± 1.54) μg/L (P < 0.05).② After GnRH-a treatment higher basal AMH level fell down,and lower basal AMH level grew up in both groups.Using rising or falling AMH level as the standard to the ROC curve,we found the cutoff point of serum AMH:2.15 μg/L in long-acting group,and 2.16 μg/L in short-acting group.③In long-acting group,based on the cutoff points,basal AMH level of 52 women was less than 2.15 μg/L,and their basal serum AMH was (1.39 ± 0.54) μg/L,after down-regulation AMH was (2.73 ±-1.83) μg/L(P <0.01); 76 women with basal serum AMH ≥2.15 μg/L,their basal serum AMH was (4.40 ± 1.95) μg/L,after down-regulation was (3.27 ± 1.78) μg/L(P < 0.01).In short-acting group,51 women with basal serum AMH < 2.16 μg/L,AMH before down-regulation was (1.21 ± 0.50) μg/L,after down-regulation was (1.96 ± 1.57) μg/L (P <0.01) ; 65 women with basal serum AMH ≥2.16 μg/L,their basal serum AMH was (4.26 ± 1.93) μg/L,after down-regulation was (2.55 ± 1.48) μg/L (P < 0.01).④In long-acting group,for those whose AMH level < 2.15 μ g/L,there was no significant difference

  13. Influence of Tiaojing Zhuyun Fang Combined with Letrozole on the Situation of Follicular Development and Anti-Mullerian Hormone Level in Infertility Patients with Polycystic Ovary Syndrome%调经助孕方联合来曲唑对多囊卵巢综合征不孕患者卵泡发育及抗苗勒氏管激素的影响

    Institute of Scientific and Technical Information of China (English)

    党慧敏; 刘艳巧; 刘润侠; 陈薇

    2013-01-01

    目的 研究中药调经助孕方联合来曲唑对多囊卵巢综合征(PCOS)不孕患者卵泡发育及抗苗勒氏管激素(AMH)的影响,探讨其作用机制.方法 将60例PCOS不孕患者随机分为3组,每组20例.中药组服用调经助孕方,西药组服用来曲唑,中西药组采用调经助孕方联合来曲唑治疗.比较3组患者治疗2个疗程后月经改善情况、基础体温(BBT)变化、排卵恢复情况、血清性激素及AMH水平.结果 治疗后中药组排卵率为45%(9例),西药组为60%(12例),中西药组为85%(17例),中西药组高于中药组及西药组(P<0.05);中药组受孕率为40%(8例),西药组为45%(9例),中西药组为80%(16例),中西药组高于中药组及西药组(P<0.05).治疗后较治疗前各组血清性激素雌二醇、孕酮水平均升高,黄体生成素、睾酮、AMH水平均降低(P<0.05).结论 调经助孕方联合来曲唑对PCOS不孕患者有较好的促排卵作用,并可提高受孕率,其作用机制可能与调节AMH水平有关.%Objective To investigate the influences of Tiaojing Zhuyun Fang (TJZYF) combined with letrozole on the situation of follicular development and anti-Mullerian hormone (AMH) level in infertility patients with polycystic ovary syndrome (PCOS), and explore its mechanism. Methods Sixty infertility patients with PCOS were divided into three groups randomly with 20 cases in each group. The Chinese medicine group was treated by TJZYF, the western medicine group was treated by letrozole, and the integrative medicine group was given both of them. The menstruation improvement, the change of the basal body temperature (BBT), spontaneous ovulation recovery, serum sexual hormones and AMH of the three groups after 2 courses of treatment were compared. Results After the treatment, the ovulation rate in the Chinese medicine group was 45% (9 cases), western medicine group was 60% (12 cases), while the integrative medicine group was 85% (17 cases). The ovulation

  14. 血清抗苗勒管激素水平在基于2012年中国多囊卵巢综合征诊断标准患者中的初步研究%A pilot study of serum anti-Mullerian hormone in diagnosis of polycystic ovary syndrome based on 2012 Chinese polycystic ovary syndrome diagnosis criteria

    Institute of Scientific and Technical Information of China (English)

    杨诗源; 阮洁; 黄薇; 谭婧; 张向卉; 冯雪; 刘冬; 傅璟; 周璐

    2015-01-01

    目的 研究血清抗苗勒管激素(AMH)在基于2012年中国多囊卵巢综合征(PCOS)诊断标准患者中的水平,初步评价血清AMH检测在PCOS诊断中的临床意义.方法 采集2015年4月至8月四川大学华西第二医院217例PCOS评分患者(PCOS组)及204例输卵管或男性因素不孕患者(对照组)的临床资料,计算体质指数,评价多毛、痤疮评分,检测血清AMH、性激素、糖脂代谢指标的水平,B超测定卵巢体积及卵泡数.采用Spearman相关性检验进行相关性分析,通过受试者工作特征曲线(ROC曲线)评价AMH的诊断效能.结果 血清AMH水平在PCOS组[105.46(78.11 ~ 135.37) pmol/L]明显高于对照组[26.28(20.00~ 43.63) pmol/L],两组比较,差异有统计学意义(P<0.01).Spearman相关性分析显示,血清AMH水平与多毛评分(r=0.526,P<0.01)、痤疮评分(r=0.359,P<0.01)、总睾酮(r=0.514,P<0.01)、LH/FSH比值(r=0.542,P<0.01)、空腹血糖(r=0.373,P<0.01)、空腹胰岛素(r=0.168,P=0.008)、胰岛素抵抗指数(r=0.182,P=0.004)、总胆固醇(r=0.247,P<0.01)、甘油三酯(r=0.235,P<0.01)、卵巢体积(r=0.204,P=0.008)、卵泡数目(r=0.693,P<0.01)呈正相关.在月经紊乱的情况下,血清AMH水平用于诊断PCOS的ROC曲线下面积为0.954(P<0.01),当AMH>57.76 pmol/L时,诊断的敏感度为95.1%,特异度为89.3%.结论 依据中国2012年PCOS诊断标准,血清AMH水平在PCOS患者中明显升高,并与临床和(或)生化高雄状况、卵巢体积、卵泡数目有较好的相关性.%Objective Based on 2012 Chinese polycystic ovary syndrome (PCOS) diagnosis criteria, we detect the serum anti-Müllerian hormone (AMH) levels in PCOS patients, to evaluate the diagnosis value of AMH for PCOS.Methods Totally 217 PCOS patients were chosen as study group and 204 non-PCOS women were as controls.Their clinical information (body weight, score for acne and hirsutism), ultrasonography for ovarian volume and the number of small follicle

  15. Anti-Mullerian Hormone (AMH)Levels and its Relationships with Insulin Resistance and Androgen in Women with Polycystic Ovary Syndrome%PCOS患者血清抗苗勒管激素(AMH)水平与胰岛素抵抗及雄激素的关系

    Institute of Scientific and Technical Information of China (English)

    肖琳; 李启富; 唐良萏

    2011-01-01

    研究多囊卵巢综合征(PCOS)患者血清抗苗勒管激素(AMH)水平的变化及其与胰岛素抵抗(IR)和雄激素的关系.方法:回顾性分析85例PCOS患者及79例正常女性的体质量指数(BMI)、血清AMH、血糖、胰岛素、雄激素、稳态模型胰岛素抵抗指数(HOMA-IR)间的相互关系.血清AMH采用ELISA法测定,空腹血浆葡萄糖(FPG)采用葡萄糖氧化酶法测定,空腹胰岛素(FIns)采用化学发光法测定,睾酮(T)采用酶联免疫法测定.结果:PCOS组血清AMH水平明显高于对照组;Person相关分析表明,PCOS组血清AMH水平与HOMA-IR及BMI不相关,与雄激素、年龄相关.多元线性回归分析显示,PCOS组中血清雄激素水平是血清AMH的影响因素.结论:PCOS人群中血清AMH较正常女性升高,但其升高与IR无关,而与雄激素水平密切相关.%Objective: To explore the relationships among insulin resistance (IR), androgens (T) and anti-Miillerian hormone (AMH) in women with polycystic ovary syndrome (PCOS). Methods: Eighty-five PCOS patients and 79 non-PCOS candidates were retrospectively analyzed. Body hight and weight were measured to calculate body mass index (BMI). BMI were calculated. AMH levels, fast blood glucose, fast blood insulin and androgen were assessed. Homeostatic model assessment of tissue insulin sensitivity (HOMA-IR) was calculated. The relationships among AMH, insulin resistance, androgen and BMI in patients with and without PCOS were studied. Results: The serum level of AMH of PCOS patients was significantly higher than that of non-PCOS patients. Age and androgen level, but not BMI and HOMA-IR, showed significant correlation with AMH level in patients with PCOS. Multiple stepwise regression analysis demonstrated that serum androgen level was independently related to serum AMH level in patients with PCOS. Conclusion: The AMH level was elevated and androgen level may relate to AMH level in patients with PCOS.

  16. Study on Reference Range of Anti-Mullerian Hormone Level in the Serum for Healthy Women of Childbearing Age in Handan Area%邯郸地区健康育龄妇女血清抗苗勒氏管激素参考值范围研究

    Institute of Scientific and Technical Information of China (English)

    杨小岗; 王芳; 武佳敏; 武春燕; 武保乡

    2012-01-01

    Objective To established the reference range of AMH in the serum by determinating the antt-Mullerian hormone level (AMH) in the serum and the relativity of reproductive age for healthy women of childbearing age in Handan area. Methods Used the Enzyme-linked Immunosorbent Assay (ELISA) to determine the AMH level of serum in the early follicle period (2~5 days menstrual cycle) or corpora luteum metaphase (4~8 days after BBT rised) .meanwhile,need to determine the six reproductive Hormone level of LH,FSH,T,E2,FRL and P for 165 cases with 20~50 years old healthy women of childbearing age. Results The AMH level of serum was distributed in the shape of abnormal status. So the reference range indicated in median (M) and scope. The AMH level of serum in the early follicle period and corpora luteum metaphase bdicated respectively 3. 87(0. 41~10. 69)ng/ml and 4. 64(0. 45—11. 64)ng/ml, which were not with the remarkable differences. The AMH level of serum in early stage and late stage indicated respectively 5.14(0.45~11.64)ng/ml and 2.02(0.41 —9. 03)ng/ml,which would with the remarkable differences (P< 0.001). The reference range of total level for AMH was 4. 55(0. 41~11. 65)ng/ml which will increase in inverse proportion to the age(r=—0. 365,P=0. 000). That was to say,as they grew older, the AMH level would reduce. Conclusion The medical reference range of AMH level in this thesis could be applied to health and medicine practice for clinical diagnosis and treatment of reproduction and infertility of women.%目的 通过测定邯郸地区健康育龄妇女血清抗苗勒氏管激素(AMH)水平及与生殖年龄的相关性,建立该地区实验室血清AMH参考值范围.方法 采用酶联免疫吸附技术(ELISA)检测165例20~50岁健康育龄妇女早卵泡期(月经周期2~5天)或黄体中期(BBT上升后4~8天)血清AMH水平,并同时检测LH,FSH,T,E2,PRL和P等六项生殖激素水平.结果 血清AMH水平呈非正态分布,故参考值范围用中位

  17. 多囊卵巢综合征患者控制性超促排卵过程 AMH 和 INHB 浓度变化的临床研究%Changes of anti-Mullerian hormone (AMH) and inhib in B (INHB) during controlled o varian hyperstimulation in polycystic ovarian syndrome

    Institute of Scientific and Technical Information of China (English)

    李莹; 聂明月; 方颖; 杨晓葵

    2014-01-01

    目的:探讨多囊卵巢综合征( polycystic ovarian syndrome , PCOS )患者控制性超促排卵( controlled ovarian hyperstimulation,COH)过程中血清抗苗勒管激素(anti-Müllerian hormone,AMH)和抑制素B(inhibin B,INHB)浓度的变化及其对COH结局的预测价值。方法 PCOS 组患者37例,对照组患者41例。检测患者月经周期第2/3天( D2/3)、促性腺激素(gonadotrophin,Gn)刺激第5天(D5)、人绒毛膜促性腺激素(human chorionic gonadotropin,hCG)日(D-hCG)血清AMH及INHB浓度及取卵日卵泡液中AMH及INHB浓度,观察其动态变化及其对COH结局的影响。结果在COH过程中,血清AMH浓度逐渐降低,而血清INHB浓度逐渐升高。 PCOS组患者hCG日血清雌二醇( estrodiol,E2)浓度、获卵数、冷冻胚胎数较对照组明显升高(P<0.05,P<0.001),而Gn用量、受精率均低于对照组,差异有统计学意义(P<0.05),2组患者的临床妊娠率比较,差异无统计学意义(P>0.05)。血清AMH和INHB浓度与Gn剂量、hCG日E 2浓度具有相关性(P<0.05,P<0.001),但是与获卵数、临床妊娠率无相关性(P>0.05)。结论 PCOS患者 AMH和INHB浓度在COH过程中呈现动态变化,可以预测COH中卵巢的反应性,但是对超促排卵结局的预测价值还有待进一步研究。%Objective To explore dynamics of anti-Müllerian hormone ( AMH ) and inhibin B ( INHB ) during controlled ovarian hyperstimulation ( COH) in polycystic ovarian syndrome ( PCOS ) , and assess the value of these dynamic changes on the prediction of outcome in PCOS patients undergoing in vitro fertilization ( IVF) .Methods Thirty-seven women with PCOS and 41 women with normal ovarian reserve as the control were recruited in the study .AMH and INHB levels were measured in serum on menstrual cycle day 2 or 3 (D2/3), day 5 of gonadotropin stimulation(D5), human chorionic

  18. Anti-mullerian hormone as a diagnostic and prognostic tool for PCOS patients

    OpenAIRE

    Wiweko, Budi; Maidarti, Mila; Priangga, M. Dwi; Shafira, Nadia; Fernando, Darrell; Sumapraja, Kanadi; Natadisastra, Muharam; Hestiantoro, Andon

    2014-01-01

    Purpose To determine whether the measurement of serum AMH can be used to diagnose PCOS and as a tool to predict the prognosis of PCOS. Methods This is a case–control study. Women of reproductive age (18–35 years) were recruited consecutively at a tertiary academic hospital during the period of March 2009–October 2011 and were divided into case (PCOS patients defined by the Rotterdam criteria) and control groups (non-PCOS patients). Menstrual history, clinical manifestations of hyperandrogenis...

  19. Which follicles make the most anti-Mullerian hormone in humans?

    DEFF Research Database (Denmark)

    Jeppesen, J V; Anderson, R A; Kelsey, T W;

    2013-01-01

    AMH concentrations. To determine AMH gene expression in GC (q-RT-PCR) and follicular AMH production (Elisa and RIA) in relation to follicular development, 87 follicles (3-13 mm diameter) including both GC and the corresponding follicular fluid (FF) were collected in connection with fertility...

  20. Comparison of anti-mullerian hormone and antral follicle count for assessment of ovarian reserve

    Directory of Open Access Journals (Sweden)

    Sonal Panchal

    2012-01-01

    Full Text Available Aim: This study aims to compare the efficacy of AFC and AMH, as markers for ovarian reserve. Materials and Methods: 75 patients with PCO (polycystic ovaries undergoing IVF were randomized with 75 non-PCO patients. On day 3, volume of ovary was acquired, ovarian volume was defined by VOCAL, and Sono AVC was used to count the number of antral follicles. Sum total of antral follicles in both ovaries was taken as total antral follicle count (AFC. AMH was measured on the same day. Long agonist protocol with recombinant FSH (rFSH was used for IVF stimulation till at least two follicles of 18 mm were seen. hCG 10,000 iu was given and ovum pick up was done after 34-35 h. Primary end point was number of follicles >12 mm seen on day of hCG. Final end point was number of ova retrieved on ovum pick up. Correlation of AFC and AMH was checked for both end points and with each other. Results: Correlation of AFC and follicles >12 mm on day of hCG in PCO group is 0.56 and non-PCO group is 0.63, 1 and for AMH and follicles >12 mm on day of hCG in PCO group is 0.42 and non-PCO group is 0.47. Correlation of AFC with number of ova retrieved on OPU in PCO group is 0.44 and for non-PCO group is 0.50. The value for AMH is 0.39 in PCO and 0.43 for non-PCO group. Comparing correlation of AFC and AMH for primary end point in PCO group has ′z′ value 1.11(onetailed significance 0.1335, twotailed significance 0.267 and in non-PCO group comparison shows a ′z′ value of 1.39 (one tailed significance 0.0823, two-tailed significance 0.1645. Therefore in both groups, AFC and AMH correlates with total number of follicles >12 mm on day of hCG, but both AFC and AMH have independent significance. Comparing correlation of AFC and AMH with number of ova retrieved on OPU, in non-PCO group has ′z′ value of 0.54(one tailed 0.2946, two-tailed 0. 5892. In PCO group, this comparison shows, ′z′ value of 0.36(one tailed 0.3594, two tailed 0.7188. Conclusion: AFC and AMH correlate with each other but have independent significance for estimating follicles >12 mm on day of hCG. For ova retrieved on OPU, in PCO group, AMH and AFC have similar significance. In non-PCO, AMH may increase accuracy of estimation of number of ova retrieved on OPU over AFC, but difference is not significant. AFC only may suffice for estimation of number of ova retrieved on OPU for all practical purposes.

  1. Research progress of anti-mullerian hormone and follicular development%抗苗勒管激素与卵泡发育的研究进展

    Institute of Scientific and Technical Information of China (English)

    王丽; 吕淑兰

    2008-01-01

    抗苗勒管激素又称苗勒抑制物质,是属于转化生长因子β(TGF-β)超家族成员之一的一种多功能细胞因子,由睾丸未成熟的Sertoli细胞和卵巢颗粒细胞分泌,具有调节两性生殖细胞生长发育及分化、性腺发育及促进雄性胚胎苗勒管退化等作用.近年来各国学者对抗苗勒管激素在多囊卵巢综合征中的作用展开了广泛的研究,了解抗苗勒管激素的分子结构、基因表达和功能,对多囊卵巢综合征患者的卵泡发育及发病机制研究有重要意义.

  2. 抗苗勒氏管激素对精子发生的影响%Influence of anti-mullerian hormone on spermatogenesis

    Institute of Scientific and Technical Information of China (English)

    赵海波; 宋晖; 于月成

    2009-01-01

    目的 检测人精浆中是否存在抗苗勒氏管激素(AMH),并分析其与精子密度、活动率及活力的关系.方法 酶联免疫吸附试验(ELISA)检测150对不孕症患者男方精浆AMH水平,精液分析异常者治疗后再次检测AMH,观察治疗前后精浆AMH水平的变化.结果 精浆中存在AMH,明显高于血清中水平(P<0.01),精液分析正常者精浆AMH水平高于精液分析异常者(P<0.01),AMH水平与精子密度及活动率呈正相关.结论 精液中存在AMH,AMH对精子密度及活动率有促进作用.

  3. Relationship between polymorphism of anti-mullerian hormone,anti-mullerian hormone receptor type Ⅱ and polycystic ovary syndrome%抗苗勒氏管激素及其Ⅱ型受体的基因多态性与多囊卵巢综合征发病的关系

    Institute of Scientific and Technical Information of China (English)

    李键; 乔杰; 闫丽盈; 唐荣欣; 甄秀梅

    2008-01-01

    目的 探讨抗苗勒氏管激素(AMH)Ile49 Ser及抗苗勒氏管激素Ⅱ型受体(AMHR2)-482A>G多态性与多囊卵巢综合征(PCOS)发病的相关性.方法 采用聚合酶链反应(PCR)和DNA测序方法,检测103例PCOS妇女和79例正常排卵妇女AMHIle49 Ser和AMHR2-482A>G的基因型分布,并采用酶放大化学发光及酶联免疫吸附试验(ELISA)等方法测定血清激素.结果 PCOS组和正常对照组的AMHIle49 Ser和AMHR2-482A>G的基因型分布比较,差异无显著性(P>0.05);PCOS组AMH的Ile/Ile、Ile/Ser和Ser/Ser3种基因型的血清AMH水平分别是(11.0±4.2)、(13.4±6.9)和(17.6±6.5)ng/ml,差异有显著性(PG多态性与PCOS发病可能不相关,PCOS组血清AMH水平明显高于正常排卵妇女,AMHIle49 Ser的多态性可能足影响PCOS妇女卵泡期AMH水平的因素之一.

  4. A New Predictor AMH (anti-müllerian hormone to Determining Ovarian Reserve and Menoposual Aging in the Women

    Directory of Open Access Journals (Sweden)

    Levent Sarýyýldýz

    2013-05-01

    Full Text Available       Anti-mullerian hormone (AMH, also termed Mullerian inhibiting substance (MIS, is produced in ovary by granulose cells and known as a member of the TGF- β superfamily. The researchers imply that AMH is associated with the various growth factors in development and growth of ovarian follicles, furthermore  is exhibited the features of other TGF-β family members. AMH has a critical importance in reproductive age of women. Certain standardization has not yet been developed in determing the ovarian reserve. Recent findings reveal the importance of AMH in determining ovary function and, highlight the importance of AMH than FSH.

  5. Hormones

    Science.gov (United States)

    Hormones are your body's chemical messengers. They travel in your bloodstream to tissues or organs. They work ... glands, which are special groups of cells, make hormones. The major endocrine glands are the pituitary, pineal, ...

  6. The diversity of anti-mullerian hormone level between different ovarian reserve female%抗苗勒管激素水平在不同卵巢储备功能女性之间的差异

    Institute of Scientific and Technical Information of China (English)

    赵玲; 肖春辉

    2013-01-01

    目的 探讨抗苗勒管激素(AMH)在多囊卵巢综合征(PCOS)、卵巢早衰(POF)、正常人群中的差异.方法 内分泌门诊就诊的PCOS患者(PCOS组)60例,卵巢早衰(POF)患者(POF组)30例,随机选择健康志愿者30例作为对照组.统计3组年龄,并计算体质量指数(AMI),测定卵泡数量(AFC)、血清(FSH)、黄体生成素(LH)、睾酮(T)、雌三醇(E2)和血清AMH水平.结果 PCOS组AFC明显高于POF组及对照组,对照组明显高于POF组,差异有统计学意义(P<0.01);PCOS组BMI明显高于POF组及对照组,差异有统计学意义(P<0.01);PCOS组T、E2、AMH明显高于POF组与对照组,对照组明显高于POF组,差异有统计学意义(P<0.01);POF组LH水平高于PCOS组和对照组,PCOS组高于对照组,差异均有统计学意义(P<0.01);POF组FSH明显高于PCOS组与对照组,差异有统计学意义(P<0.01).结论 AMH在PCOS、POF、及正常生育年龄女性间存在显著差异,可作为临床评估卵巢储备功能的指标.

  7. 两种药物促排卵过程中PCOS患者AMH变化%Anti-Mullerian Hormone Plasma Levels during Treatment with Two Different Drugs to Induce Ovulation in Women with Polycystic Ovary Syndrome

    Institute of Scientific and Technical Information of China (English)

    田玉燕; 李晓丹; 徐素欣

    2008-01-01

    目的:探讨来曲唑(LE)和尿促性腺激素(HMG)治疗多囊卵巢综合征(PCOS)患者在促排卵过程中抗苗勒氏管激素(AMH)的变化.方法:选择PCOS患者50例,随机分为2组,分别给予来曲唑2.5 mg/d口服,共5 d;或低剂量递增法肌注hMG;对照组50例;阴道超声计数小卵泡数和优势卵泡数,测定月经第3天、第8天以及hCG日(对照组LH峰日)血AMH、E2.结果:在促排卵过程中,hMG组AMH水平逐渐降低,与E2升高间成负相关(r=0.65);LE组AMH水平略有下降但无显著性;对照组AMH值无明显变化.结论:LE治疗过程中PCOS患者AMH变化及卵泡生长更接近自然月经周期.

  8. Relationship study between anti-mullerian hormone level in human follicular fluid and oocyte%人卵泡液中抗苗勒管激素水平与卵母细胞关系的研究

    Institute of Scientific and Technical Information of China (English)

    赵海波; 宋晖; 于月成

    2009-01-01

    目的 探讨卵泡液中抗苗勒管激素(AMH)水平对卵母细胞发育、受精和卵裂的影响.方法 采用酶联免疫吸附法(ELISA)检测60例体外受精-胚胎移植患者卵泡液中AMH水平,分析其与卵母细胞发育、受精和卵裂的关系.结果 卵泡液AMH水平为(56.7±11.2)pmol/L,显著高于血清AMH水平((20.7±5.5)pmol/L,P<0.01).含成熟卵母细胞的卵泡液AMH水平为(57.3±12.1)pmol/L,高于含未成熟卵母细胞者((55.8±9.3)pmol/L,P<0.05);受精卵的AMH水平((57.9±11.7)pmol/L)高于未受精卵((54.5±6.6)pmol/L,P<0.01);Ⅰ级和Ⅱ级胚胎的AMH水平高于Ⅲ级和Ⅳ级胚胎(P均<0.05).结论 卵泡液中AMH可促进卵母细胞发育,提高受精卵发育潜能.

  9. 抗苗勒氏管激素早期预测卵巢储备功能的研究%Studies of anti-mullerian hormone on early prediction of the ovarian reserve functions

    Institute of Scientific and Technical Information of China (English)

    王瑜; 韩玉芬; 张晨静; 王志勇

    2012-01-01

    目的:探讨抗苗勒氏管激素(AMH)早期预测卵巢储备功能下降的临床价值及给予相应干预治疗后对其妊娠率的影响.方法:通过检测血清抗苗勒氏管激素,筛查出202例因卵巢储备功能下降导致不孕的患者,随机分为A、B两组,A组未经治疗直接促排卵,B组经人工周期治疗后促排卵,观察两组周期妊娠率、临床妊娠率及B组治疗前后激素水平的变化情况.结果:A组周期妊娠率、临床妊娠率均低于B组,差异均有统计学意义(P<0.05);B组中AMH水平治疗后较治疗前升高,差异有统计学意义(P<0.05).结论:血清AMH水平可作为临床早期预测卵巢储备功能下降的指标之一;早期诊断卵巢储备功能下降并给予合理的性激素治疗可使卵巢功能得到改善,妊娠率得到提高.

  10. Prediction of Ovarian Reserve with Anti-mullerian Hormone, Inhibin B and Ultrasound%抗苗勒管激素、抑制素B及超声预测卵巢储备功能

    Institute of Scientific and Technical Information of China (English)

    石洁; 张云山

    2009-01-01

    近年来,辅助生殖技术(ART)已成为治疗不孕、不育症的主要手段之一.在体外受精-胚胎移植周期中,正确评价卵巢储备功能及卵巢对控制性超促排卵治疗的反应性,有利于从疗效的角度筛选患者,制定最佳个体化超促排卵治疗方案,防止卵巢过度刺激征(OHSS)发生,减少患者不必要的经济和精神损失,进一步提高临床妊娠率.评价卵巢储备功能的指标较多,其可靠性还存在较多的差异,抗苗勒管激素(AMH)、抑制素B(INHB)及超声检查在预测卵巢储备功能方面发挥着重要的作用.

  11. 宫颈妊娠病人双侧子宫动脉栓塞术前后血清AMH检测%SERUM ANTI-MULLERIAN HORMONE CONCENTRATION IN PATIENTS WITH CERVICAL PREGNANCY BEFORE AND AFTER BILATERAL UTERINE ARTERY EMBOLIZATION

    Institute of Scientific and Technical Information of China (English)

    左建新; 任立盛; 邵翠华; 刘美新; 王言奎

    2014-01-01

    目的 通过血清抗苗勒管激素(AMH)的测定,评价双侧子宫动脉栓塞术对卵巢储备功能的影响.方法 采用Beckman Coulter ELISA法检测20例宫颈妊娠病人双侧子宫动脉栓塞术前及术后1、6个月血清AMH水平,并比较其变化.结果 宫颈妊娠病人子宫动脉栓塞术前至术后1、6个月血清AMH水平分别为(7.99±4.15)、(7.11±3.02)和(8.33±4.87)mg/L,手术前后比较差异无显著性(F=0.93,P>0.05).结论 子宫动脉栓塞术治疗宫颈妊娠不影响卵巢储备功能.

  12. Induction of WNT inhibitory factor 1 expression by Müllerian inhibiting substance/AntiMullerian hormone in the Müllerian duct mesenchyme is linked to Müllerian duct regression

    OpenAIRE

    Park, Joo Hyun; Tanaka, Yoshihiro; Arango, Nelson A.; Zhang, Lihua; Benedict, L. Andrew; Roh, Mi In; Donahoe, Patricia K.; Teixeira, Jose M.

    2013-01-01

    A key event during mammalian sexual development is regression of the Müllerian ducts (MDs) in the bipotential urogenital ridges (UGRs) of fetal males, which is caused by the expression of Müllerian inhibiting substance (MIS) in the Sertoli cells of the differentiating testes. The paracrine signaling mechanisms involved in MD regression are not completely understood, particularly since the receptor for MIS, MISR2, is expressed in the mesenchyme surrounding the MD, but regression occurs in both...

  13. The relationship between anti-mullerian hormone and PCOS%抗苗勒管激素与多囊卵巢综合征相关性分析

    Institute of Scientific and Technical Information of China (English)

    李琳; 陈晓莉; 陈亚肖; 李予; 钟俊敏; 杨冬梓

    2009-01-01

    目的 研究多囊卵巢综合征(PCOS)血清抗苗勒管激素(AMH)水平及其与PCOS各项指标的相关性.方法 选择2006年6~12月在中山大学附属第二医院妇产科就诊的47例PCOS患者为研究组,收集同期年龄匹配的40例健康女性为对照组,应用酶联免疫吸附(ELISA)法检测血清AMH.结果 研究组的血清AMH明显高于对照组[(9.85±4.93)ug/L对(7.13±3.02)ug/L,P=0.002).成年期研究组血清AMH亦明显高于对照组[(10.64±5.47)ug/L对(7.01±2.43)ug/L,P=0.005),而青春期研究组血清AMH虽较对照组增高,但差异无统计学意义[(8.95±4.18)ug/L对(7.26±3.58)ug/L,P=0.168).Pearson相关分析显示血清AMH与年龄无相关性,与雄激素和其他内分泌等各项指标无相关性,与平均卵巢体积呈正相关(r=0.319,P=0.029),但与平均卵泡数目无相关性.结论 PCOS患者的血清AMH明显增高,其与血清雄激素相关性不明显,提示AMH有可能通过某种机制在卵泡局部发挥作用,影响卵泡发育而参与PCOS的病理生理过程.

  14. 抗苗勒氏管激素与多囊卵巢综合征的相关性研究%Study on the relationship between serum anti-mullerian hormone and polycystic ovary syndrome

    Institute of Scientific and Technical Information of China (English)

    董晨; 李红发

    2006-01-01

    目的 探讨抗苗勒氏管激素(AMH)与多囊卵巢综合征(PCOS)发生、发展的关系.方法 测定51例PCOS患者血清AMH、卵泡刺激素、黄体生成素、催乳素、睾酮、雌二醇水平以及双侧卵巢卵泡数(FN),并取20例月经周期正常妇女进行对照.结果 PCOS组血清AMH(31.10±13.44)pmol/L,明显高于对照组(12.01±4.44)pmol/L,差异有极显著性,并且两组血清AMH水平都与FN呈正相关.除此之外,PCOS患者血清AMH水平还与睾酮水平呈显著正相关,且这种相关关系并不出现在对照组.结论 AMH可能参与PCOS卵泡发育异常并与性激素合成失调有关.测定血清AMH水平为诊断和研究PCOS提供了一个重要的突破点.

  15. 血清抗苗勒管激素在预测围绝经期女性卵巢功能中的应用%Application of anti-mullerian hormone serum in predicting ovarian functions of perimenopausal women

    Institute of Scientific and Technical Information of China (English)

    杨冰; 叶元; 秦辛玲; 石青峰

    2013-01-01

    目的 观察血清抗苗勒管激素(AMH)在围绝经期女性中的变化,探讨AMH用于评估卵巢功能的意义.方法 选取月经失调患者60例,根据卵巢功能分为围绝经期组和卵巢早衰组(POF组),各30例;选取同期月经周期正常、无内分泌疾病、年龄在35 ~ 45岁的健康妇女30例,作为对照组.分别于就诊当月或下月月经周期第2~3天(闭经者日期不限),检测各组血清AMH、卵泡刺激素(FSH)、黄体生成激素(LH)、雌二醇(E2)水平,其中围绝经期组6、12个月之后重复检测上述项目.结果 与对照组比较,POF组血清AMH、E2水平下降,FSH、LH水平上升(P均<0.05);与对照组比较,围绝经期组血清AMH下降(P<0.05),FSH水平稍上升,LH、E2在正常水平(P均>0.05);POF组血清AMH水平低于围绝经期组(P<0.05);围绝经期组6个月后重复检测,血清AMH水平较就诊时下降(P<0.05),而FSH、LH、E2水平变化不明显(P均>0.05);12个月后,血清AMH水平进一步下降,FSH、LH水平上升,E2水平下降,P均<0.05.结论 血清AMH比FSH、LH、E2更早反映卵巢功能下降,能更早预测围绝经期妇女卵巢功能衰退.

  16. 抗苗勒管激素与多囊卵巢不孕患者有无排卵的相关关系%Relationship between serum anti - mullerian hormone and ovulation in women with polycystic ovaries

    Institute of Scientific and Technical Information of China (English)

    李键; 乔杰; 赵淑云; 王静; 严杰; 李蓉

    2008-01-01

    目的 研究抗苗勒管激素(AMH)与多囊卵巢(PCO)不孕患者有无排卵的关系,探讨AMH在卵巢卵泡生长和发育停滞中的作用.方法 采用酶联免疫吸附法(ELISA)检测35例正常卵巢形态的排卵妇女(A组)、29例PCO伴正常排卵妇女(B组)和38例PCO伴排卵障碍妇女(C组)的月经第2 d(d2)血清及排卵前卵泡液(FF)的AMH水平.结果 B组和C组的月经d2血清AMH水平比较.差异无显著性(5.87ng/ml和7.99 ng/ml;P>0.05),但显著高于A组(2.52 ng/ml;P0.05),但显著高于A组(29.45 ng/g pro;P<0.05).结论 多囊卵巢患者血清AMH水平升高,其颗粒细胞可能存在AMH分泌异常,但多囊卵巢患者AMH水平的增高可能不是造成卵泡生长停滞的原因.

  17. Changes in Anti-Mullerian Hormone (AMH) throughout the Life Span: A Population-Based Study of 1027 Healthy Males from Birth (Cord Blood) to the Age of 69 Years

    DEFF Research Database (Denmark)

    Aksglæde, Lise; Sørensen, K; Boas, M.;

    2010-01-01

    the ontogeny of AMH secretion through life in healthy males. Setting:This was a population-based study of healthy volunteers. Participants: Participants included 1027 healthy males from birth (cord blood) to 69 yr. A subgroup was followed up longitudinally through the infantile minipuberty [(in cord blood...

  18. Prediction of assisted reproductive technique outcome in elevated early follicular phase follicle stimulating hormone with Mullerian inhibiting substance level

    Directory of Open Access Journals (Sweden)

    Leili Safdarian

    2012-01-01

    Full Text Available Background: Detection of best predictor of ovarian reserve in patients with temporarily or consistently elevated early follicular phase serum levels of FSH is one of the most important goals in assisted reproductive technique (ART.Objective: To evaluate whether high level of anti-mullerian hormone level is related to success of ART in patients with temporarily or consistently elevated early follicular phase serum levels of FSH.Materials and Methods: Sixty three women underwent intracytoplasmic sperm injection (ICSI with GnRH-agonist long protocol or intrauterine insemination (IUI in a prospective cohort study. FSH, inhibin B and anti-Mullerian hormone (AMH levels were measured in these women whom were divided to three groups (persistently elevated FSH, variably elevated FSH and, normal FSH level. Basal characteristics, stimulation parameters, and pregnancy occurrence were evaluated.Results: AMH was significantly higher in women with persistently elevated early follicular phase FSH achieving pregnancy. Women with normal FSH did not have significant difference in AMH level between conceived and non conceived cycles. Women with only one elevated early follicular phase FSH achieving pregnancy did not have significant difference in AMH level with non pregnant women. Response to gonadotropin stimulation, recommendation to oocyte donation significantly differed between the groups.Conclusion: This study has demonstrated that relatively young women with persistently or intermittently elevated day 3 FSH levels have diminished ovarian reserve and lower ART success. However, in women whose FSH levels were constantly elevated, AMH (not inhibin B concentrations were significantly higher in ART cycles resulting in pregnancy. Therefore, AMH level is a good predictor of ART outcome in patients with elevated early follicular phase serum levels of FSH.

  19. Naturopathy and yoga in ameliorating multiple hormonal imbalance: a single case report

    Directory of Open Access Journals (Sweden)

    Pradeep M.K. Nair

    2016-03-01

    Full Text Available Hormonal imbalances are common among the women who are in the halfway of their reproductive age. There are lot of factors like stress, diet, lifestyle etc. which contributes to this hormonal dysfunctions. However these factors are merely addressed by the existing management strategies. A 37 year old female presented with hypothyroidism and associated hyperprolactinemia had undergone Naturopathy and yoga interventions for a period of 18 months. Her baseline TSH and prolactin levels were 9.2 U/ml and 34 ng/ml respectively. Her anti-mullerian hormone (AMH levels also fell down to 0.3 ng/ml. Naturopathy and yoga based lifestyle interventions including hydrotherapy, mud therapy, yoga therapy and acupuncture were given for different durations has streamlined the hormone levels to normal range (TSH-4.6 U/ml, prolactin- 19.6 ng/ml, AMH-2.6 ng/ml. The results indicate that naturopathy and yoga has a positive role in reinstating the hormonal homeostasis. However large scale studies are warranted to bestow better care. [Int J Reprod Contracept Obstet Gynecol 2016; 5(3.000: 916-918

  20. 促性腺激素释放激素拮抗剂对卵巢颗粒细胞分泌抗苗勒管激素的影响%Effect of gonadotropin releasing hormone antagonist on the secretion of anti-Mullerian hormone of granulosa cells

    Institute of Scientific and Technical Information of China (English)

    张英; 陆芝英; 王婷; 王佳佳; 华克勤

    2014-01-01

    目的:研究促性腺激素释放激素拮抗剂(GnRH-ant)对卵巢颗粒细胞分泌抗苗勒管激素(AMH)的影响.方法:从人卵泡液中分离、培养颗粒细胞.分别用生理盐水(NS)、顺铂(cisplatin,DDP,5μg/ml)、cetrorelix(GnRH-ant,10-9mol/L)或cetrorelix (10-9mol/L)+DDP(5μg/ml)处理颗粒细胞24 h后,ELISA方法检测颗粒细胞分泌AMH的水平.裸鼠动物随机分为4组:NS组:皮下注射NS0.1 ml/天;1周后腹腔注射NS 0.2 ml/周.DDP组:皮下注射NS 0.1 ml/天;1周后腹腔注射5 mg/kg DDP/周.cetrorelix组:皮下注射100 μg cetrorelix/天;1周后腹腔注射NS 0.2 ml/周.cetrorelix+DDP组:皮下注射100 μg cetrorelix/天;1周后腹腔注射5 mg/kg DDP/周.用药第29天,经眼球取血后用ELISA方法检测各组裸鼠血清AMH的水平.结果:成功分离、培养人卵巢颗粒细胞.与DDP组及NS组比较,cetrorelix组及cetrorelix+ DDP组颗粒细胞AMH分泌明显增加(P<0.05).Cetrorelix组及cetrorelix+ DDP组裸鼠血清AMH水平明显高于DDP组(P<0.05).结论:GnRH-ant能够上调颗粒细胞分泌AMH,对抗DDP对AMH的降调节作用.

  1. Correlation of anti-Mullerian hormone and baseline follicle-stimulating hormone levels in women with regular menstruation%月经规律女性抗缪勒氏管激素与基础卵泡刺激素关系的研究

    Institute of Scientific and Technical Information of China (English)

    宁宁; 张杰; 王社教; 尉春艳; 陈庆; 何长武

    2016-01-01

    目的 探讨月经规律女性抗缪勒氏管激素(AMH)随年龄变化规律及其与基础卵泡刺激素(bFSH)之间的关系.方法 以89例月经规律女性为研究对象,按年龄分为5组(20 ~24岁组,25 ~29岁组,30 ~ 34岁组,35 ~ 39岁组,40~44岁组),应用全自动电化学发光法测定血清中AMH及bFSH水平,分析AMH及bFSH随年龄的变化规律及其相互间的关系.结果 AMH水平与年龄呈负相关(r=-0.847,P<0.001);bFSH与年龄呈正相关(r=0.296,P=0.005);除第2、3组间AMH无明显差异外(P=0.091),其余各组间均有显著性差异(P1∶2<0.001,P1∶3<0.001,P1∶4<0.001,P1∶5<0.001,P2∶4<0.001,P2∶5<0.001,P3∶4 =0.003,P3∶5 <0.001,P4∶5<0.001);血清AMH水平与bFSH呈负相关(r=-0.406,P<0.05).结论 月经规律女性AMH水平随年龄增加逐渐下降,其与年龄相关性优于bFSH,且AMH水平可预测bFSH水平,在评价卵巢功能中起重要作用.

  2. 血清抗苗勒管激素水平与多囊卵巢综合征激素特征关系的研究%Relationship between the level of serum anti - mullerian hormone and hormone characteristics in polycystic ovary syndrome

    Institute of Scientific and Technical Information of China (English)

    孙静; 熊晓英; 齐国华; 王颖; 张树芝; 胡舒勤; 孟军; 赖雪娜

    2009-01-01

    目的 探讨抗苗勒管激素(AMH)与多囊卵巢综合征(PCOS)卵泡发育和激素特征的关系.方法 测定47例Pcos患者血清AMH、卵泡刺激素、黄体生成素、催乳素、睾酮、雌二醇水平以及双侧卵巢卵泡数(FN),并取23例月经周期正常妇女进行对照.结果 PCOS组血清AMH(32.57±13.31)pmol/L、T(3.01±0.12)nmol/L、LH(17.68±3.11)U/L水平,明显高于对照组AMH(13.06±4.59)pmol/L、T(1.31±0.02)nmol/L、LH(9.07±2.09)U/L,两组差异有统计学意义(P<0.01),并且两组血清AMH水平都与FN呈正相关.结论 血清AMH异常可能是PCOS卵泡发育异常和性激素合成失调的原因之一;测定血清AMH水平为诊断和研究PCOS提供了一个重要的方法.

  3. The expression of serum anti-mullerian hormone Serum in different crowd and its correlation research%抗苗勒管激素在卵巢储备功能不同患者中的表达及相关性研究

    Institute of Scientific and Technical Information of China (English)

    田春花; 胡蓉; 罗艳; 李彩艳

    2011-01-01

    目的 通过测定卵巢早衰(POF)、多囊卵巢综合征(PCOS)及正常对照组患者血清中抗苗勒管激素(AMH)水平,探讨血清AMH在不同卵巢储备功能患者中的不同表达.方法 测定POF组(25例)、PCOS组(32例)和正常对照组(30例)血清中AMH水平,同时测定上述三组人群血清中卵泡刺激素(FSH)、黄体生成素(LH)、雌二醇(E2)、睾酮(T)及泌乳素(PRL)水平,并记录受试者的年龄、体重指数(MBI)、空腹血糖及胰岛素水平.结果 ①三组中POF血清AMH值最低,PLOS最高,且差异有统计学意义(P<0.05);②三组血清中FSH、LH、PRL、E2 、T及AMH水平差异均有统计学意义(P<0.05),而这三组间年龄、MBI差异无统计学意义(P>0.05);③PCOS组血清中AMH与LH、T及MBI呈正相关性 (P<0.05),与FSH和胰岛素抵抗指数无相关性.结论 在不同卵巢储备功能患者中血清AMH表达不同,可能参与PCOS发生、发展,并与性激素合成失调有关.

  4. 1400名0~5岁健康儿童血清抗苗勒氏管激素及抑制素B临床检测研究%A clinical study on the detection of serum anti-Mullerian hormone and inhibin B in 1400 cases of healthy children aged 0 to 5 years

    Institute of Scientific and Technical Information of China (English)

    2016-01-01

    目的 检测0~5岁健康儿童血清抗苗勒氏管激素(AMH)及抑制素B(INHB)浓度,为建立正常参考范围提供帮助.方法 选择来自上海市儿童医院、深圳市儿童医院、天津医科大学总医院、江西省儿童医院及成都市妇女儿童中心医院的健康体检男童及女童各700名,共1400名.根据年龄分为7组:0~1个月;>1~6个月;>6个月至1岁;>1~2岁;>2~3岁;>3~4岁;>4~5岁,其中每组男童及女童各100名.采用酶联免疫吸附法(ELISA)检测血清AMH浓度(μg/L)及INHB浓度(ng/L).结果 0~5岁健康男童血清AMH浓度在>1~6个月达高峰210.70(55.37~439.45) μg/L,>1~5岁维持平稳低水平147.10(17.69 ~ 321.88) ng/L;女童血清AMH浓度在>1~6个月也出现小高峰1.77 (0.06 ~ 7.68) ng/L,>2~5岁趋于平稳为1.98(0.19 ~ 7.31) ng/L.男童血清INHB浓度在>1~6个月达高峰274.23(0.00~602.34) ng/L,女童>1~6个月出现小高峰为36.83(0.00~226.32) ng/L.结论 提供了多中心及大样本0~5岁健康儿童血清AMH及INHB值的浓度范围,并发现>1~6个月年龄段出现浓度高峰.

  5. Analysis on anti-mullerian hormone, antiendometrial antibody and anti-ovary antibody of female patients with infertility%女性不孕患者抗苗勒管激素、抗子宫内膜抗体和抗卵巢抗体检测分析

    Institute of Scientific and Technical Information of China (English)

    马芳芳; 王厚照

    2016-01-01

    目的 探讨抗苗勒管激素(AMH)、抗卵巢抗体(AOAb)、抗子宫内膜抗体(AEAb)女性不孕的关系方法 采用酶联免疫吸附实验 ELISA 和化学发光法对 800 例女性不孕患者和850例有生育史妇女进行AMH、AOAb和AEAb检测.结果 女性不孕患者抗苗勒管激素AMH水平为6.01±1.17 ng/mL,明显高于对照组(P<0.05);抗子宫内膜抗体EMAb阳性率为10.25%,抗卵巢抗体AOAb阳性率为6.38%,阳性率都明显高于对照组(P<0.05).结论 AMH、AOAb和AEAb与女性不孕密切相关,对临床诊断和治疗具有重要的指导意义.

  6. Value of serum anti-Mullerian hormone measurement in predicting ovarian response in controlled ovarian hyperstimulation%血清抗苗勒氏管激素在超促排卵周期中预测卵巢反应性的临床研究

    Institute of Scientific and Technical Information of China (English)

    刘阳; 蔡霞; 杜红; 玛依热·吐尔逊

    2010-01-01

    目的 探讨血清抗苗勒氏管激素水平(AMH)在辅助生殖超排卵周期中预测卵巢反应性的价值.方法 按获卵数及人绒毛膜促性腺激素(hCG)注射日雌二醇(E2)水平,将68例接受体外受精-胚胎移植(IVF-ET)/体外受精-胞浆内单精子注射(ICSl)的不孕症患者分为卵巢低反应组(A组)、卵巢正常反应组(B组)及卵巢高反应组(C组).于促排周期月经第2天及hCG注射日,分别检测血清AMH、E2及孕酮(P)水平.比较3组临床效果及注射rFSH后AMH、E2、黄体生成素(LH)及P水平的变化.按妊娠结局分为妊娠组和非妊娠组,比较AMH、窦卵泡数(AFC)、优质胚胎率、优质胚胎数的变化.结果 ①A、B、C 3组月经第2天(D2)AMH水平的M分别为0.645、1.762及3.101 ng/mL;IVF周期hCG日AMH水平M分别为0.331、0.898及1.434 ng/mL,3组比较差异有统计学意义(P<0.001);多重比较后均发现D2AMH及hCG日AMH差异有统计学意义(P<0.001)(C组>B组>A组).②获卵数与促排周期D2AMH、hCG日AMH、AFC(窦卵泡数)、P呈显著正相关;与促排周期D2卵泡刺激(FSH)、促性腺激素(GN)量呈负相关.③卵巢过度刺激综合征(OHSS)的发生和GN量、AFC、D2AMH、HCG日E2及HCG日AMH水平均成正相关;与D2FSH成负相关.④妊娠组和未妊娠组间的卵裂率、优胚率及优质胚胎数间有差异性(P<0.05).结论 ①D2AMH、hCG日的AMH水平及AFC均是预测卵巢反应性较好的标记物;②D2AMH、hCG日AMH及hCG日E2水平对预测OHSS的发生具有一定的指导意义;③AMH水平不能预测妊娠结局.

  7. Impact of laparoscopic ovarian drilling on anti-Mullerian hormone levels in women with polycystic ovary syndrome%腹腔镜卵巢打孔术对多囊卵巢综合征患者抗苗勒激素水平的影响

    Institute of Scientific and Technical Information of China (English)

    饶龙泉

    2013-01-01

    目的 评估腹腔镜卵巢打孔术(LOD)对多囊卵巢综合征(PCOS)患者抗苗勒激素(AMH)水平的影响.方法 对35位PCOS妇女行腹腔镜卵巢打孔术,并观察血清抗苗勒激素水平和妊娠率.结果 PCOS组患者血清AMH是正常对照组妇女的4.6倍.妊娠PCOS患者LOD前后AMH水平降低36%.结论 检测LOD治疗的PCOS妇女的血清AMH水平,将有助于预测LOD结局.

  8. Prediction value of serum anti-Mullerian hormone on ovarian response in controlled ovarian hyperstimulation in IVF%血清抗苗勒氏管激素在体外受精超排卵中预测卵巢反应的价值

    Institute of Scientific and Technical Information of China (English)

    王馥新; 杨辰; 吴惠华; 邹琴燕; 孟庆霞; 许咏乐; 王玮; 李红

    2013-01-01

    目的 血清抗苗勒管激素(AMH)由窦前和小窦卵泡分泌,是一个反映卵巢储备的指标.分析AMH在正常育龄人群、多囊卵巢综合征(PCOS)患者、卵巢储备功能减退(DOR)人群中的分布规律,探讨AMH预测卵巢反应的价值. 方法 回顾分析2008年6月至2012年6月在我中心接受诊治的患者,将其分为三组:(1)正常组:293例.(2) PCOS组:258例.(3)DOR组:59例.比较AMH水平在这三组间的差异,绘制ROC曲线选择判断PCOS及DOR的最佳临界值.进一步选取同时间段,在我中心初次行长方案体外受精/卵胞浆内单精子注射-胚胎移植(IVF/ICSI-ET)的患者,578例.按获卵数分为卵巢低反应组(0~3个)、正常反应组(4~19个)、高反应组(≥20个),评估年龄、基础窦卵泡计数(AFC)、基础卵泡刺激素(FSH)、黄体生成素(LH)、雌二醇(E2)水平,以及AMH、促性腺激素(Gn)用量与卵巢反应的关系.绘制相对工作特征(ROC)曲线,寻找AMH在IVF中判断卵巢反应的界点. 结果 AMH水平随年龄增加而下降.AMH水平在PCOS组中较正常组显著升高,在DOR组中明显降低.25~40岁人群中,当AMH界值取1.26 ng/ml时,判断DOR的敏感性及特异性分别为88%及90%.当AMH界值取3.97 ng/ml时,判断PCOS的敏感性及特异性分别为74%及63%.在IVF中,AFC和AMH水平在三组间均有显著差异(P<0.05).当AMH界值为1.84 ng/ml时,判断该患者本周期为低反应的敏感性及特异性分别为89%及75%,当AMH界值为3.95 ng/ml时,判断为高反应的敏感性及特异性为79%及70%. 结论 AMH反映卵巢储备状态.在IVF超排卵中,AMH能较好地预测卵巢反应性.

  9. The correlation between anti-Mullerian hormone and insulin resistance in rats with polycystic ovary syndrome%多囊卵巢综合征大鼠抗苗勒管素与胰岛素抵抗水平相关性分析

    Institute of Scientific and Technical Information of China (English)

    孙秀红; 韦相才

    2011-01-01

    目的 探讨多囊卵巢综合征(PCOS)大鼠血清及卵巢单卵泡抗苗勒管素(AMH)水平与胰岛素抵抗(IR)水平间的相关性.方法 采用孕激素联合绒毛膜促性腺激素(hCG)方法制作SD大鼠PCOS模型,测定大鼠血清AMH、黄体生成素(LH)、卵泡刺激素(FSH)、空腹血糖(FPG)及空腹胰岛素(FINS),计算LH/FSH、HOMA指数,根据HOMA指数分为PCOS无IR组和PCOS合并IR组,并与对照组分析比较,行相关性和回归分析;用免疫组织化学法对不同组单卵泡AMH水平行半定量分析.结果 实验组血清AMH水平均显著高于对照组(P=0.043),PCOS无IR组和PCOS并IR组血清AMH水平与HOMA指数呈正相关(r=0.691,P=0.001);血清AMH与HOMA指数和FINS之间有直线回归关系(F=12.362,P=0.000);免疫组化显示3组间单卵泡AMH水平差异无统计学意义(P=0.720).结论 PCOS大鼠模型中AMH和胰岛素共同影响卵泡生长发育,AMH和胰岛素水平呈正相关性;卵巢局部单卵泡抗苗勒管素表达水平差异无统计学意义.

  10. Chronic Pelvic Inflammation Diminished Ovarian Reserve as Indicated by Serum Anti Mulerrian Hormone.

    Directory of Open Access Journals (Sweden)

    Linlin Cui

    Full Text Available To explore the potential damaging effect of chronic pelvic inflammation on ovarian reserve.Case-control study.A total of 122 women with bilateral tubal occlusion, diagnosed by hysterosalipingography (HSG and 217 women with normal fallopians were recruited.Serum anti-Mullerian hormone (AMH, basic follicle-stimulating hormone (FSH, luteining hormone (LH, estradiol (E2, and testosterone (T were measured; and antral follicle counts (AFCs were recorded.Significantly lower level of AMH was observed in women with bilateral tubal occlusion compared to control group [2.62 (2.95 ng/ml vs. 3.37 (3.11 ng/ml, P = 0.03], and the difference remained after adjustment of BMI (Padjust = 0.04. However, no statistical difference was found in the levels of FSH [7.00 (2.16 IU/L vs. 6.74 (2.30 IU/L], LH [4.18 (1.52 IU/L vs. 4.63 (2.52 IU/L], E2 [35.95 (20.40 pg/ml vs. 34.90 (17.85 pg/ml], T [25.07±11.46 ng/dl vs. 24.84±12.75 ng/dl], and AFC [6.00 (4.00 vs. 7.00 (4.00] between two groups (p>0.05.Women with bilateral tubal occlusion showed decreased AMH level, suggesting that chronic pelvic inflammation may diminish ovarian reserve. More caution should be paid when evaluating the detriment of PID on female fertility.

  11. Does AMH Reflect Follicle Number Similarly in Women with and without PCOS?

    Directory of Open Access Journals (Sweden)

    Sverre C Christiansen

    Full Text Available Increased Anti-Mullerian Hormone in polycystic ovary syndrome, may be due to overactive follicles rather than reflect antral follicle count.Does Anti-Mullerian Hormone reflect antral follicle count similarly in women with or without polycystic ovary syndrome or polycystic ovarian morphology?Cross-sectional, case-control.Women who delivered preterm in 1999-2006. For each index woman, a woman with a term delivery was identified.Participation rate was 69%. Between 2006-2008, 262 women were included, and diagnosed to have polycystic ovary syndrome, polycystic ovarian morphology or to be normal controls.Blood tests, a clinical examination and vaginal ultrasound.Anti-Mullerian Hormone/antral follicle count-ratio, SHBG, androstenedione and insulin, to test potential influence on the Anti-Mullerian Hormone/antral follicle count -ratio.Mean Anti-Mullerian Hormone/antral follicle count ratio in women with polycystic ovary syndrome or polycystic ovarian morphology was similar to that of the controls (polycystic ovary syndrome: 1,2 p = 0,10 polycystic ovarian morphology: 1,2, p = 0,27 Controls 1,3. Anti-Mullerian Hormone showed a positive linear correlation to antral follicle count in all groups. Multivariate analysis did not change the results.We confirmed the positive correlation between AMH and follicle count. Anti-Mullerian Hormone seems to be a reliable predictor of antral follicle count, independent of polycystic ovary syndrome diagnosis or ovarian morphology.

  12. Primary Ovarian Insufficiency (POI)

    Science.gov (United States)

    ... HCP may also check other hormones including an “AMH” (Anti-Mullerian Hormone) level and genetic tests to ... there is a medical reason for the POI. AMH is made by the follicles (within the ovaries). ...

  13. ANTI-MÜLLERIAN HORMONE AND INHIBIN-B LEVEL PROFILE IN CERVICAL CANCER PATIENTS TREATED WITH PACLITAXEL AND CISPLATIN COMBINATION

    Directory of Open Access Journals (Sweden)

    N. Abdulah

    2014-06-01

    Full Text Available Background: The age of nulliparous women has increased in developed countries and the 5-year survival rate for the late stage does not exceed 58%. Moreover, as more women are delaying child-bearing, preservation of fertility and reproductive function is a major concern when young women are counseled with regard to the effects of treatment for cervical cancer. Objective: To determine the effect of the combination chemotherapy on serum levels of Anti-Mullerian Hormone (AMH and inhibin B. Methods: This study is a prospective cohort study in 16 cervical cancer patients aged 28-48 years who received Paclitaxel-Cisplatin chemotherapy. AMH and inhibin B levels were examined before and after third series chemotherapy. Statistical analysis used the Wilcoxon test with a level of significance selected at 0.05. Results: There is a significant decrease in median serum levels of AMH and inhibin B patients between before and after Paclitaxel–Cisplatin chemotherapy with p=0.000 (P <0.05. Similarly, a decrease in the median value of serum levels of AMH and inihibin B after Paclitaxel-Cisplatin chemotherapy in each series of chemotherapy was significant with p=0.000 (P <0.05. Conclusions: Serum levels of AMH and inhibin B in cervical cancer patients who received the combination chemotherapy decreased dramatically after 3 months of chemotherapy and the factors that contribute to the diminution is age.

  14. Growth Hormone

    Science.gov (United States)

    ... page: Was this page helpful? Also known as: GH; Human Growth Hormone; HGH; Somatotropin; Growth Hormone Stimulation Test; Growth Hormone ... I should know? How is it used? Growth hormone (GH) testing is primarily used to identify growth hormone ...

  15. 抗苗勒管激素与女性生殖功能障碍及性早熟'%Evolution of Anti-müllerian Hormone in Female Dysgenesia and Precocious Puberty

    Institute of Scientific and Technical Information of China (English)

    田春花

    2011-01-01

    抗苗勒管激素(anti-mullerian hormone,AMH)是转化生长因子β超家族的成员,在女性中仅表达于卵巢的颗粒细胞,可用于评估卵巢的储备功能.其有Ⅰ~Ⅲ型受体,现已明确人类的AMH通过其II型受体(AMHR Ⅱ)发挥作用.目前多囊卵巢综合征(PCOS)、卵巢功能早衰(POF)是引起育龄期妇女不孕的主要原因.AMH在PCOS患者血清中浓度较高;而在POF患者血清中浓度较低,甚至检测不到;在性早熟(PA)患儿血清中的浓度接近正常.研究发现,AMH及AMHR Q的基因多态性与PCOS和POF的发病密切相关.AMH可作为PCOS,POF和PA等疾病的诊断依据,并可指导PCOS患者的临床治疗.

  16. Growth Hormone Ameliorates the Radiotherapy-Induced Ovarian Follicular Loss in Rats: Impact on Oxidative Stress, Apoptosis and IGF-1/IGF-1R Axis.

    Directory of Open Access Journals (Sweden)

    Yasmen F Mahran

    Full Text Available Radiotherapy is one of the standard cytotoxic therapies for cancer. However, it has a profound impact on ovarian function leading to premature ovarian failure and infertility. Since none of the currently available methods for fertility preservation guarantees future fertility, the need for an effective radioprotective agent is highly intensified. The present study investigated the mechanisms of the potential radioprotective effect of growth hormone (GH on γ irradiation-induced ovarian failure and the impact of the insulin like growth factor 1 (IGF-1 in the underlying protection. Immature female Sprague-Dawley rats were either exposed to single whole body irradiation (3.2 Gy and/or treated with GH (1 mg/kg s.c. Experimental γ-irradiation produced an array of ovarian dysfunction that was evident by assessment of hormonal changes, follicular development, proliferation marker (PCNA, oxidative stress as well as apoptotic markers. In addition, IGF-1/IGF-1R axis expression was assessed using real-time PCR and immunolocalization techniques. Furthermore, after full maturity, fertility assessment was performed. GH significantly enhanced follicular development and restored anti-Mullerian hormone serum level as compared with the irradiated group. In addition, GH significantly ameliorated the deleterious effects of irradiation on oxidative status, PCNA and apoptosis. Interestingly, GH was shown to enhance the ovarian IGF-1 at transcription and translation levels, a property that contributes significantly to its radioprotective effect. Finally, GH regained the fertility that was lost following irradiation. In conclusion, GH showed a radioprotective effect and rescued the ovarian reserve through increasing local IGF-1 level and counteracting the oxidative stress-mediated apoptosis.

  17. Bioidentical Hormones and Menopause

    Science.gov (United States)

    ... Balance › Bioidentical Hormones and Menopause Fact Sheet Bioidentical Hormones and Menopause January, 2012 Download PDFs English Espanol ... take HT for symptom relief.) What are bioidentical hormones? Bioidentical hormones are identical to the hormones that ...

  18. Hormone Data

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Hormones quantified from marine mammal and sea turtle tissue provide information about the status of each animal sampled, including its sex, reproductive status and...

  19. 血清抗苗勒管激素水平检测在预测卵巢低反应中的应用价值%Value of serum anti-Müllerian hormone levels for predicting low ovarian response

    Institute of Scientific and Technical Information of China (English)

    廖月婵; 吴日然; 柯玩娜; 林晓丽; 叶云; 杜彦; 杜静; 林秀峰

    2012-01-01

    Objective: To evaluate the value of serum anti-Mullerian hormone (AMH) in predicting ovarian response to controlled ovarian hyperstimulation (COH) during in vitro fertilization. Methods: A total of 335 COH cycles including 6,5 cycles with poor ovarian response (the number of retrieved oocytes ≤5) and 243 cycles with normal response (the number of retrieved oocytes ≥5) were retrospectively evaluated from January to June, 2010. The serum levels of AMH, FSH, LH and E2 were measured on day 2-5 in spontaneous menstrual cycle of the patients. The patients were received transvagi-nal ultrasound examination, and their antral follicle count (AFC) was assayed. Results: The number of retrieved oocytes was correlated with AMH, AFC, age, FSH/LH, basal FSH levels, basal LH levels, and there is a gradual tendency from strong to weak. The area under the receive operating characteristic curve (ROC) of AMH, AFC, basal FSH levels, FSH/LH, age, basal LH levels, basal E2 levels was from large to small. The cut-off value of anti-Mullerian hormone for predicting ovarian response was equal or more than 2. 21 μg/L with 77% of sensitivity and 80% of specificity. Conclusions: Serum AMH levels might be a better parameter for predicting ovarian response. It could be used as a parameter for selecting the scheme of COH to reduce the cancellation rate of poor responders.%目的 探讨血清抗苗勒管激素(AMH)水平对于卵巢储备功能低下患者在控制性超排卵(COH)中卵巢反应的应用价值. 方法 回顾性分析2010年1月至6月在本中心接受体外受精/卵胞浆内单精子注射-胚胎移植(IVF/ICSI-ET)治疗的308周期.患者分为卵巢低反应组(65例)和卵巢正常反应组(243例).清晨空腹静脉血测定AMH(无月经周期限制)和卵泡刺激素(FSH)、黄体生成素(LH)、雌二醇(E2)水平(月经第2~5天),同时行阴道B超检查测定窦卵泡数(AFC),以获卵数为评价标准. 结果 与获卵数的相关性由强到弱是AMH水平

  20. Early testicular effects in rats perinatally exposed to DEHP in combination with DEHA - apoptosis assessment and immunohistochemical studies

    DEFF Research Database (Denmark)

    Borch, Julie; Dalgaard, Majken; Ladefoged, Ole

    2005-01-01

    This study aimed to characterize the effects of di(2-ethylhexyl) phthalate (DEHP) on the fetal rat testes and relate them to the effects seen in adults. Histopathological effects in fetal testes were examined with immunohistochemistry for anti-Mullerian hormone (ANTH), 3 beta-hydroxysteroid dehyd......This study aimed to characterize the effects of di(2-ethylhexyl) phthalate (DEHP) on the fetal rat testes and relate them to the effects seen in adults. Histopathological effects in fetal testes were examined with immunohistochemistry for anti-Mullerian hormone (ANTH), 3 beta...

  1. 抗苗勒氏管激素(AMH)对离体人卵巢黄素化颗粒细胞干细胞因子(SCF)表达负调控的研究%Impact of Anti Miillerian Hormone(AMH) on the Expression of Stem Cell Factor (SCF) in Human Granulosa Cells Cultured in Vitro

    Institute of Scientific and Technical Information of China (English)

    胡蓉; 王飞苗; 罗艳; 马会明; 吴昕

    2011-01-01

    Objective: To explore the influence of anti Mullerian hormone (AMH) on the expression of SCF in human granulosa cells cultured in vitro. Methods: Fifteen patients were recruited who received in vitro fertilization and embryo transfer (IVF-ET) at age 0.05). Conclusion: AMH can effectively down-regulate the expressions of SCF mRNA and protein in human granulosa cells.%目的:研究人卵巢黄素化颗粒细胞中抗苗勒氏管激素(AMH)对干细胞因子(SCF)表达的影响.方法:收集15例年龄<35岁因男方因素行体外受精-胚胎移植(IVF-ET)患者的卵巢颗粒细胞,经原代培养,加入不同浓度基因重组人抗苗勒氏管激素(rhAMH),分别于培养的第4日与第6日采用实时定量PCR(RT-PCR)和免疫组织化学检测空白对照组与各实验组SCF mRNA及蛋白的表达.结果:RT-PCR和免疫组织化学均证实人卵巢黄素化颗粒细胞在不同浓度rhAMH干预后,SCFmRNA及蛋白的表达显著减少(P<0.05),其中15 ng/ml rhAMH抑制作用最明显;实验组第4日与第6日颗粒细胞表达SCFmRNA及蛋白无明显差异(P>0.05).结论:AMH可有效抑制人卵巢黄素化颗粒细胞SCF mRNA及蛋白的表达.

  2. Hormone impostors

    Energy Technology Data Exchange (ETDEWEB)

    Colborn, T.; Dumanoski, D.; Myers, J.P.

    1997-01-01

    This article discusses the accumulating evidence that some synthetic chemicals disrupt hormones in one way or another. Some mimic estrogen and others interfere with other parts of the body`s control or endocrine system such as testosterone and thyroid metabolism. Included are PCBs, dioxins, furans, atrazine, DDT. Several short sidebars highlight areas where there are or have been particular problems.

  3. Endocrine Glands & Their Hormones

    Science.gov (United States)

    ... Characteristics of Hormones Endocrine Glands & Their Hormones Pituitary & Pineal Glands Thyroid & Parathyroid Glands Adrenal Gland Pancreas Gonads Other ... hormone secretion. « Previous (Characteristics of Hormones) Next (Pituitary & Pineal Glands) » Contact Us | Privacy Policy | Accessibility | FOIA | File Formats ...

  4. Influence of gonadotropin-releasing hormone agonist on the effect of chemotherapy upon ovarian cancer and the prevention of chemotherapy-induced ovarian damage: an experimental study with nu/nu athymic mice

    Institute of Scientific and Technical Information of China (English)

    Qiong-yan LIN; Yi-feng WANG; Hui-nan WENG; Xiu-jie SHENG; Qing-ping JIANG; Zhi-ying YANG

    2012-01-01

    Background and objective:Gonadotropin-releasing hormone (GnRH) plays an important role in the regulation of ovarian function and ovarian cancer cell growth.In this study,we determined whether administration of the GnRH agonist (GnRHa),triporelin,prior to cisplatin treatment affects cisplatin and/or prevents cisplatin-induced ovarian damage.Methods:nu/nu mice were injected with ovarian cancer OVCAR-3 cells intraperitoneally.After two weeks,the mice were treated with saline (control),cisplatin,GnRHa,or cisplatin plus GnRHa for four weeks.At the end of the experimental protocol,blood,tumor,ovary,and uterine tissues were resected for hematoxylin and eosin (H&E) staining,immunohistochemical analyses of Ki67,nuclear factor-κB (NF-κB),and caspase-3,transmission electron microscopy of apoptosis,or enzyme-linked immunosorbent assay (ELISA) analyses of anti-Mullerian hormone (AMH).Results:Cisplatin treatment effectively inhibited tumor growth in mice treated with human ovarian cancer cells; however the treatment also induced considerable toxicity.Immunohistochemical analyses showed that Ki67 expression was reduced in cisplatin-treated mice compared to control (P<0.05),but there was no statistically significant differences between cisplatin-treated mice and cisplatin plus GnRHa-treated mice (P>0.05),while expressions of NF-κB and caspase-3 were reduced and induced,respectively,in cisplatin-treated mice and cisplatin plus GnRHa-treated mice.Apoptosis occurred in the GnRHa,cisplatin,and cisplatin plus GnRHa-treated mice,but not in control mice.Ovaries exposed to GnRHa in both GnRHa mice and cisplatin-treated mice (combination group) had significantly more primordial and growth follicles and serum levels of AMH than those in the control mice and cisplatin-treated mice (P<0.05).Conclusions:Administration of GnRHa to mice significantly decreased the extent of ovarian damage induced by cisplatin,but did not affect the anti-tumor activity of cisplatin.

  5. Hormone Replacement Therapy

    Science.gov (United States)

    ... before and during menopause, the levels of female hormones can go up and down. This can cause ... hot flashes and vaginal dryness. Some women take hormone replacement therapy (HRT), also called menopausal hormone therapy, ...

  6. Thyroid Hormone Treatment

    Science.gov (United States)

    ... Giving Workplace Giving Other Ways to Donate Thyroid Hormone Treatment Thyroid hormone is used in two situations: ... prevent recurrence or progression of their cancer. THYROID HORMONE REPLACEMENT THERAPY Many people have a thyroid gland ...

  7. Growth hormone test

    Science.gov (United States)

    ... page: //medlineplus.gov/ency/article/003706.htm Growth hormone test To use the sharing features on this page, please enable JavaScript. The growth hormone test measures the amount of growth hormone in ...

  8. AMH and AFC as predictors of excessive response in controlled ovarian hyperstimulation : a meta-analysis

    NARCIS (Netherlands)

    Broer, S. L.; Dolleman, M.; Opmeer, B. C.; Fauser, B. C.; Mol, B. W.; Broekmans, F. J. M.

    2011-01-01

    background: Anti-Mullerian hormone (AMH) is a marker of ovarian reserve status and represents a good predictor of ovarian response to ovarian hyperstimulation. The aim of this study was to assess the accuracy of AMH and antral follicle count (AFC) as predictors of an excessive response in IVF/ICSI t

  9. Sertoli Cell Differentiation in Pubertal Boars

    Science.gov (United States)

    Meishan boars experience puberty at a younger age than crossbred (BX) boars in association with earlier cessation of Sertoli cell proliferation and smaller post pubertal testicular size. The current study defined changes in expression, assessed by immunohistochemistry, of anti-Mullerian hormone (AMH...

  10. Relationship of antral follicle counts to fertility and ovarian AMH mRNA levels in beef cows

    Science.gov (United States)

    In mammalian females, reproductive senescence is associated with decreased numbers of follicles in the ovary. Anti-Mullerian Hormone (AMH) of ovarian origin has been proposed to be a biomarker of the number of follicles in the ovary and of fertility in women, as well as to control activation of pri...

  11. [Hormonal dysnatremia].

    Science.gov (United States)

    Karaca, P; Desailloud, R

    2013-10-01

    Because of antidiuretic hormone (ADH) disorder on production or function we can observe dysnatremia. In the absence of production by posterior pituitary, central diabetes insipidus (DI) occurs with hypernatremia. There are hereditary autosomal dominant, autosomal recessive or X- linked forms. When ADH is secreted but there is an alteration on his receptor AVPR2, it is a nephrogenic diabetes insipidus in acquired or hereditary form. We can make difference on AVP levels and/or on desmopressine response which is negative in nephrogenic forms. Hyponatremia occurs when there is an excess of ADH production: it is a euvolemic hypoosmolar hyponatremia. The most frequent etiology is SIADH (syndrome of inappropriate secretion of ADH), a diagnostic of exclusion which is made after eliminating corticotropin deficiency and hypothyroidism. In case of brain injury the differential diagnosis of cerebral salt wasting (CSW) syndrome has to be discussed, because its treatment is perfusion of isotonic saline whereas in SIADH, the treatment consists in administration of hypertonic saline if hyponatremia is acute and/or severe. If not, fluid restriction demeclocycline or vaptans (antagonists of V2 receptors) can be used in some European countries. Four types of SIADH exist; 10 % of cases represent not SIADH but SIAD (syndrome of inappropriate antidiuresis) due to a constitutive activation of vasopressin receptor that produces water excess. c 2013 Published by Elsevier Masson SAS. PMID:24356291

  12. Ultrasound in evaluating ovarian reserve

    OpenAIRE

    Eman Ahmaed Shawky Sabek; Ola I. Saleh; Howida A. Ahmed

    2015-01-01

    The objective of this study was to compare the diagnostic accuracy of transvaginal ultrasound (TVS), as a less invasive technique instead of hormonal assay to evaluate the ovarian reserve. This study included fifty-five females with breast cancer and we compared the ovarian reserve for these patients by hormonal assay through measuring the serum AntiMullerian Hormone (AMH) level and follicular stimulating hormone (FSH) level before and after chemotherapy, and by transvaginal ultrasound throug...

  13. Correlation between ultrasound parameters and hormone level in patients with polycystic ovary and polycystic ovary syndrome%多囊卵巢与多囊卵巢综合征的超声特征和激素水平相关性分析

    Institute of Scientific and Technical Information of China (English)

    赵庆红; 石华; 杨菁; 胡佳琪; 张玉国; 陈茜; 刘艳

    2014-01-01

    将超声诊断为卵巢多囊样改变的104例患者按临床症状及内分泌检查分为多囊卵巢综合征(PCOS)组与单纯卵巢多囊样改变(PCO)组,同时纳入有正常排卵的妇女40人,做对照组,分别测定其卵巢面积(TA)、髓质面积(SA)及二者的比值(SA/TA),分析激素水平与SA/TA的相关性.PCO组的卵巢TA、SA及SA/TA、血清睾酮、苗勒氏管激素AMH及抑制素B均低于PCOS组,但高于对照组,差异具有统计学意义(P<0.05).PCOS患者血清睾酮水平与卵巢超声参数SA/TA有显著相关性(P<0.05).PCOS患者的AMH和抑制素B高于PCO组和对照组,说明AMH和抑制素B与卵巢多囊样改变的程度有关,可以用于鉴别PCOS和PCO.同时,SA/TA与PCOS血清睾酮升高有关,可以作为鉴别PCOS和PCO的内分泌指标.%A total of 104 patients with polycystic ovarian changes on ultrasound were divided into polycystic ovary syndrome (PCOS) and polycystic ovaries (PCO) groups according to their clinical symptoms and endocrine examinations.And 40 women with normal ovulation served as the controls.Ovarian volume (OV),ovarian stroma area (SA),ovarian total area (TA) and SA/TA ratio were measured by transvaginal uhrasonography.Their endocrine hormone levels were determined.Patients with PCOS showed significantly higher OV,SA,TA and SA/TA than those of PCO and control groups.SA/TA was positively correlated with testosterone.The median serum levels of anti-Mullerian hormone (AMH) and inhibit B in the PCO group were intermediate between those in control and PCOS groups.AMH and inhibin B of PCOS were higher than the PCO and control groups.It indicated that the levels of AMH and inhibit B were associated with the degree of polycystic ovaries and they could be used to differentiate PCOS and PCO.SA/TA is associated with the elevated serum testosterone of PCO.And SA/TA is an important ultrasonic parameter for diagnosing PCOS.

  14. Hormone Health Network

    Science.gov (United States)

    ... Cuidadores Hormones and Health Journey Through the Endocrine System Endocrine Disrupting Chemicals (EDCs) Endocrine Glands and Types of ... Health Hormones and Health Journey Through the Endocrine System Endocrine Disrupting Chemicals (EDCs) Endocrine Glands and Types of ...

  15. Hormones and Obesity

    Science.gov (United States)

    ... Balance › Hormones and Obesity Fact Sheet Hormones and Obesity March, 2010 Download PDFs English Espanol Editors Caroline Apovian, MD Judith Korner, MD, PhD What is obesity? Obesity is a chronic (long-term) medical problem ...

  16. Menopause and Hormones

    Science.gov (United States)

    ... Consumer Information by Audience For Women Menopause and Hormones: Common Questions Share Tweet Linkedin Pin it More ... reproduction and distribution. Learn More about Menopause and Hormones Menopause--Medicines to Help You Links to other ...

  17. Hormones and Hypertension

    Science.gov (United States)

    Fact Sheet Hormones and Hypertension What is hypertension? Hypertension, or chronic (long-term) high blood pressure, is a main cause of ... tobacco, alcohol, and certain medications play a part. Hormones made in the kidneys and in blood vessels ...

  18. Hormone therapy in acne

    OpenAIRE

    Chembolli Lakshmi

    2013-01-01

    Underlying hormone imbalances may render acne unresponsive to conventional therapy. Relevant investigations followed by initiation of hormonal therapy in combination with regular anti-acne therapy may be necessary if signs of hyperandrogenism are present. In addition to other factors, androgen-stimulated sebum production plays an important role in the pathophysiology of acne in women. Sebum production is also regulated by other hormones, including estrogens, growth hormone, insulin, insulin-l...

  19. Hormonal therapies in acne.

    Science.gov (United States)

    Shaw, James C

    2002-07-01

    Hormones, in particular androgen hormones, are the main cause of acne in men, women, children and adults, in both normal states and endocrine disorders. Therefore, the use of hormonal therapies in acne is rational in concept and gratifying in practice. Although non-hormonal therapies enjoy wide usage and continue to be developed, there is a solid place for hormonal approaches in women with acne, especially adult women with persistent acne. This review covers the physiological basis for hormonal influence in acne, the treatments that are in use today and those that show promise for the future. The main treatments to be discussed are oral contraceptives androgen receptor blockers like spironolactone and flutamide, inhibitors of the enzyme 5 alpha-reductase and topical hormonal treatments. PMID:12083987

  20. Anti-Müllerian Hormone as a Biomarker of the Status of Ovarian Reserve after Chemotherapy in Rat Model%抗苗勒氏管激素作为预测化疗后卵巢储备功能状态的实验性研究

    Institute of Scientific and Technical Information of China (English)

    吕向阳; 孙海梅; 路欣; 翁静; 梁元晶; 周德山

    2011-01-01

    目的:探讨化疗时抗苗勒氏管激素(anti-Mullerian hormone,AMH)作为预测大鼠卵巢储备功能状态的可行性.方法:雌性SD大鼠24只,随机分为3组:对照组(NS)、顺铂化疗低剂量组(4.5 mg/kg,CL)和高剂量组(6.0 mg/kg,CH),每组8只.免疫组织化学染色检测AMH在卵巢的表达,光学显微镜下计数AMH阳性卵泡百分比,ELISA检测血清AMH、FSH水平.结果:各组大鼠卵巢组织均可见AMH表达,主要位于初级卵泡、窦前卵泡及小窦状卵泡的颗粒细胞,给予顺铂12d后,AMH的表达强度减弱、范围缩小.CL组和CH组的AMH阳性卵泡百分比均较NS组明显降低(P

  1. Hormones and the pilosebaceous unit

    OpenAIRE

    Chen, Wen-Chieh; Zouboulis, Christos C

    2009-01-01

    Hormones can exert their actions through endocrine, paracrine, juxtacrine, autocrine and intracrine pathways. The skin, especially the pilosebaceous unit, can be regarded as an endocrine organ meanwhile a target of hormones, because it synthesizes miscellaneous hormones and expresses diverse hormone receptors. Over the past decade, steroid hormones, phospholipid hormones, retinoids and nuclear receptor ligands as well as the so-called stress hormones have been demonstrated to play pivotal rol...

  2. Hormonal therapy for acne.

    Science.gov (United States)

    George, Rosalyn; Clarke, Shari; Thiboutot, Diane

    2008-09-01

    Acne affects more than 40 million people, of which more than half are women older than 25 years of age. These women frequently fail traditional therapy and have high relapse rates even after isotretinoin. Recent advances in research have helped to delineate the important role hormones play in the pathogenesis of acne. Androgens such as dihydrotestosterone and testosterone, the adrenal precursor dehydroepiandrosterone sulfate, estrogens, growth hormone, and insulin-like growth factors may all contribute to the development of acne. Hormonal therapy remains an important part of the arsenal of acne treatments available to the clinician. Women dealing with acne, even those without increased serum androgens, may benefit from hormonal treatments. The mainstays of hormonal therapy include oral contraceptives and antiandrogens such as spironolactone, cyproterone acetate, or flutamide. In this article, we discuss the effects of hormones on the pathogenesis of acne, evaluation of women with suspected endocrine abnormalities, and the myriad of treatment options available. PMID:18786497

  3. Hormones and endometrial carcinogenesis.

    Science.gov (United States)

    Kamal, Areege; Tempest, Nicola; Parkes, Christina; Alnafakh, Rafah; Makrydima, Sofia; Adishesh, Meera; Hapangama, Dharani K

    2016-02-01

    Endometrial cancer (EC) is the commonest gynaecological cancer in the Western World with an alarmingly increasing incidence related to longevity and obesity. Ovarian hormones regulate normal human endometrial cell proliferation, regeneration and function therefore are implicated in endometrial carcinogenesis directly or via influencing other hormones and metabolic pathways. Although the role of unopposed oestrogen in the pathogenesis of EC has received considerable attention, the emerging role of other hormones in this process, such as androgens and gonadotropin-releasing hormones (GnRH) is less well recognised. This review aims to consolidate the current knowledge of the involvement of the three main endogenous ovarian hormones (oestrogens, progesterone and androgens) as well as the other hormones in endometrial carcinogenesis, to identify important avenues for future research. PMID:26966933

  4. Adult growth hormone deficiency

    OpenAIRE

    Vishal Gupta

    2011-01-01

    Adult growth hormone deficiency (AGHD) is being recognized increasingly and has been thought to be associated with premature mortality. Pituitary tumors are the commonest cause for AGHD. Growth hormone deficiency (GHD) has been associated with neuropsychiatric-cognitive, cardiovascular, neuromuscular, metabolic, and skeletal abnormalities. Most of these can be reversed with growth hormone therapy. The insulin tolerance test still remains the gold standard dynamic test to diagnose AGHD. Growth...

  5. Modelling plant hormone gradients.

    OpenAIRE

    S. Moore; Zhang, X.; Liu, J; Lindsey, K.

    2015-01-01

    Cellular patterning in the Arabidopsis root is coordinated via a localised auxin concentration maximum in the root tip, requiring the regulated expression of specific genes. The activities of plant hormones such as auxin, ethylene and cytokinin depend on cellular context and exhibit either synergistic or antagonistic interactions. Due to the complexity and nonlinearity of spatiotemporal interactions between both hormones and gene expression in root development, modelling plant hormone gradien...

  6. Plant Hormone Binding Sites

    OpenAIRE

    Napier, Richard

    2004-01-01

    • Aims Receptors for plant hormones are becoming identified with increasing rapidity, although a frustrating number remain unknown. There have also been many more hormone‐binding proteins described than receptors. This Botanical Briefing summarizes what has been discovered about hormone binding sites, their discovery and descriptions, and will not dwell on receptor functions or activities except where these are relevant to understand binding.

  7. Luteinizing hormone (LH) blood test

    Science.gov (United States)

    ICSH - blood test; Luteinizing hormone - blood test; Interstitial cell stimulating hormone - blood test ... medicines you take. These include: Birth control pills Hormone therapy Testosterone DHEA (a supplement) If you are ...

  8. Aging changes in hormone production

    Science.gov (United States)

    ... this page: //medlineplus.gov/ency/article/004000.htm Aging changes in hormone production To use the sharing ... that produce hormones are controlled by other hormones. Aging also changes this process. For example, an endocrine ...

  9. Hormone therapy for prostate cancer

    Science.gov (United States)

    ... this page: //medlineplus.gov/ency/patientinstructions/000908.htm Hormone therapy for prostate cancer To use the sharing ... helps slow the growth of prostate cancer. Male Hormones and Prostate Cancer Androgens are male sex hormones. ...

  10. Hormones and female sexuality

    Directory of Open Access Journals (Sweden)

    Bjelica Artur L.

    2003-01-01

    Full Text Available Introduction In contrast to animal species in which linear relationships exist between hormonal status and sexual behaviour sexuality in human population is not determined so simply by the level of sexual steroids. The article analyses female sexuality in the light of hormonal status. Administration of sexual steroids during pregnancy and sexual differentiation High doses of gestagens, especially those with high androgen activity, widely used against miscarriages may lead to tomboys, but without differences in sexual orientation. However, it has been observed that the frequency of bisexual and lesbian women is higher in women with congenital adrenogenital syndrome. Hormones sexual desire and sexuality during menstrual cycle It has been established that sexual desire, autoeroticism and sexual fantasies in women depend on androgen levels. There are a lot of reports claiming that sexual desire varies during the menstrual cycle. Hormonal contraception and sexuality Most patients using birth control pills present with decreased libido. But, there are reports that progestagens with antiandrogenic effect in contraceptive pills do not affect sexual desire. Hormonal changes in peri- and postmenopausal period and sexuality Decreased levels of estrogen and testosterone in older women are associated with decreased libido, sensitivity and erotic stimuli. Sexuality and hormone replacement therapy Hormonal therapy with estrogen is efficient in reference to genital atrophy, but not to sexual desire. Really increased libido is achieved using androgens. Also, therapy with dehydroepiandrosterone (DHEA and tibolone have positive effects on female libido. Conclusion Effect of sexual steroids on sexual sphere of women is very complex. The association between hormones and sexuality is multidimensional, as several hormones are important in regulation of sexual behaviour. Still, it should be pointed out that sexuality is in the domain of hormonal, emotional

  11. 围绝经期气郁质性激素与舌苔脱落细胞成熟指数、成熟价值的相关性%Correlation between Sex Hormones and Tongue Exfoliated Cells Maturation Index and Maturity Value in Perimenopause Women with Qi Stagnation

    Institute of Scientific and Technical Information of China (English)

    李红; 陈以君; 任林; 李灿东

    2012-01-01

    目的 探讨围绝经期气郁质性激素及舌苔脱落细胞特点,以期为围绝经期气郁质的早期辨识提供帮助.方法 选取围绝经期平和质、气郁质妇女180例,根据月经情况分为绝经前期、绝经过渡期早期、绝经过渡期晚期,每种体质每期各30例,观察两组各期卵泡刺激素(FSH)、黄体生成素(LH)、雌二醇(E2)、泌乳素(PRL)、抑制素B(InhB)、抗苗勒管激素(AMH)、舌苔脱落细胞成熟指效(MI)和成熟价值(MV),并分析各指标与气郁质的相关性.结果两组同期间FSH、LH、E2、PRL水平差异均具有统计学意义(P<0.05或P<0.01),气郁质组各指标的变化均较平和体质明显;两组FSH、LH、MV均随着围绝经期的进程而明显升高(P<0.01),E2、PRL、AMH、InhB则均明显下降(P<0.01). 结论 围绝经期气郁质妇女围绝经期进程较平和质快,且性激素、MI、MV改变与气郁质有明显相关性,可作为围绝经期气郁质早期判定的参考指标.%Objective To research the characteristics of sex hormones and exfoliated cell of tongue fur in qi stagnation constitution during the perimenopause and provide help for the early identification. Methods Totally 180 women of mild constitution and qi stagnation constitution were selected and divided into premenopause. early menopausal transition and late menopaus-al transition according to the menstruation. Thirty cases were selected in each constitution group and during each period. The correlation between qi stagnation constitution and follicle stimulating hormone (FSH), luteinizing hormone (LH), estradiol (E2), prolactin (PRL), inhibin B (InhB), anti-Mullerian hormone (AMH) and the maturation index (MI) and maturity value (MV) of tongue off cell were analyzed. Results The differences of FSH, LH, E2 and PRL were statistically significant between two constitution groups during each period (P<0.01), especially in qi stagnation constitution group. FSH, LH and MV significantly

  12. LH (Luteinizing Hormone) Test

    Science.gov (United States)

    ... reason for the delayed puberty. Some of the causes for delayed puberty can include: Failure of the ovaries or testicles Hormone deficiency Turner syndrome Klinefelter syndrome Chronic infections Cancer Eating disorder (anorexia nervosa) ^ Back to top Is there anything ...

  13. Growth hormone deficiency - children

    Science.gov (United States)

    ... be done include: Insulin-like growth factor 1 (IGF-1) and insulin-like growth factor binding protein ... themselves the shot. Treatment with growth hormone is long-term, often lasting for several years. During this ...

  14. Hormones and female sexuality

    OpenAIRE

    Bjelica Artur L.; Kapamadžija Aleksandra; Maticki-Sekulić Milana

    2003-01-01

    Introduction In contrast to animal species in which linear relationships exist between hormonal status and sexual behaviour sexuality in human population is not determined so simply by the level of sexual steroids. The article analyses female sexuality in the light of hormonal status. Administration of sexual steroids during pregnancy and sexual differentiation High doses of gestagens, especially those with high androgen activity, widely used against miscarriages may lead to tomboys, but with...

  15. Bioidentical Hormone Therapy

    OpenAIRE

    Files, Julia A.; Ko, Marcia G.; Pruthi, Sandhya

    2011-01-01

    The change in hormonal milieu associated with perimenopause and menopause can lead to a variety of symptoms that can affect a woman's quality of life. Postmenopausal hormone therapy (HT) is an effective, well-tolerated treatment for these symptoms. However, combined HT consisting of conjugated equine estrogen and medroxyprogesterone acetate has been associated with an increased number of health risks when compared with conjugated equine estrogen alone or placebo. As a result, some women are t...

  16. Gastrointestinal hormones regulating appetite

    OpenAIRE

    Chaudhri, Owais; Small, Caroline; Bloom, Steve

    2006-01-01

    The role of gastrointestinal hormones in the regulation of appetite is reviewed. The gastrointestinal tract is the largest endocrine organ in the body. Gut hormones function to optimize the process of digestion and absorption of nutrients by the gut. In this capacity, their local effects on gastrointestinal motility and secretion have been well characterized. By altering the rate at which nutrients are delivered to compartments of the alimentary canal, the control of food intake arguably cons...

  17. Body segments and growth hormone.

    OpenAIRE

    Bundak, R; Hindmarsh, P. C.; Brook, C G

    1988-01-01

    The effects of human growth hormone treatment for five years on sitting height and subischial leg length of 35 prepubertal children with isolated growth hormone deficiency were investigated. Body segments reacted equally to treatment with human growth hormone; this is important when comparing the effect of growth hormone on the growth of children with skeletal dysplasias or after spinal irradiation.

  18. Treatment with thyroid hormone.

    Science.gov (United States)

    Biondi, Bernadette; Wartofsky, Leonard

    2014-06-01

    Thyroid hormone deficiency can have important repercussions. Treatment with thyroid hormone in replacement doses is essential in patients with hypothyroidism. In this review, we critically discuss the thyroid hormone formulations that are available and approaches to correct replacement therapy with thyroid hormone in primary and central hypothyroidism in different periods of life such as pregnancy, birth, infancy, childhood, and adolescence as well as in adult patients, the elderly, and in patients with comorbidities. Despite the frequent and long term use of l-T4, several studies have documented frequent under- and overtreatment during replacement therapy in hypothyroid patients. We assess the factors determining l-T4 requirements (sex, age, gender, menstrual status, body weight, and lean body mass), the major causes of failure to achieve optimal serum TSH levels in undertreated patients (poor patient compliance, timing of l-T4 administration, interferences with absorption, gastrointestinal diseases, and drugs), and the adverse consequences of unintentional TSH suppression in overtreated patients. Opinions differ regarding the treatment of mild thyroid hormone deficiency, and we examine the recent evidence favoring treatment of this condition. New data suggesting that combined therapy with T3 and T4 could be indicated in some patients with hypothyroidism are assessed, and the indications for TSH suppression with l-T4 in patients with euthyroid multinodular goiter and in those with differentiated thyroid cancer are reviewed. Lastly, we address the potential use of thyroid hormones or their analogs in obese patients and in severe cardiac diseases, dyslipidemia, and nonthyroidal illnesses.

  19. Hormonal control of inflammatory responses

    OpenAIRE

    Garcia-Leme, J.; Farsky, Sandra P

    1993-01-01

    Almost any stage of inflammatory and immunological responses is affected by hormone actions. This provides the basis for the suggestion that hormones act as modulators of the host reaction against trauma and infection. Specific hormone receptors are detected in the reactive structures in inflamed areas and binding of hormone molecules to such receptors results in the generation of signals that influence cell functions relevant for the development of inflammatory responses. Diversity of hormon...

  20. PERSISTENT MULLERIAN DUCT SYNDROME: A CASE REPORT

    Directory of Open Access Journals (Sweden)

    Siddalingeshwar V

    2014-11-01

    Full Text Available : A 3-year-old boy presenting with right inguinal hernia was found to have uterus, fallopian tube and testes in a position analogous to ovaries in hernial sac, warranting a diagnosis of persistent Mullerian duct syndrome (PMDS. PMDS is an extremely rare form of internal male pseudo-hermaphroditism in which female internal sex organs, including the uterus, cervix and proximal vagina, persist in a 46XY male with normal external genitalia. The condition results from a congenital insensitivity to anti-Mullerian hormone, or lack of anti-Mullerian hormone, leading to persistence of the female internal sex organs in a male. Clinically, this condition is associated with cryptochoridism. Controversy persists regarding the appropriate treatment of PMDS, since resection of the remnant structures is associated with potential morbidity, but retention risks development of occasional malignancies. We review the literature and discuss various aspects of pathophysiology, diagnosis, and management of PMDS.

  1. Headache And Hormones

    Directory of Open Access Journals (Sweden)

    Shukla Rakesh

    2002-01-01

    Full Text Available There are many reasons to suggest a link between headache and hormones. Migraine is three times common in women as compared to men after puberty, cyclic as well as non-cyclic fluctuations in sex hormone levels during the entire reproductive life span of a women are associated with changes in frequency or severity of migraine attack, abnormalities in the hypothalamus and pineal gland have been observed in cluster headache, oestrogens are useful in the treatment of menstrual migraine and the use of melatonin has been reported in various types of primary headaches. Headache associated with various endocrinological disorders may help us in a better understanding of the nociceptive mechanisms involved in headache disorders. Prospective studies using headache diaries to record the attacks of headache and menstrual cycle have clarified some of the myths associated with menstrual migraine. Although no change in the absolute levels of sex hormones have been reported, oestrogen withdrawal is the most likely trigger of the attacks. Prostaglandins, melatonin, opioid and serotonergic mechanisms may also have a role in the pathogenesis of menstrual migraine. Guidelines have been published by the IHS recently regarding the use of oral contraceptives by women with migraine and the risk of ischaemic strokes in migraineurs on hormone replacement therapy. The present review includes menstrual migraine, pregnancy and migraine, oral contraceptives and migraine, menopause and migraine as well as the hormonal changes in chronic migraine.

  2. Vitamin D: potential clinical decisions in doctor practice

    OpenAIRE

    Комісаренко, Ю. І.; Бобрик, М. І.; Сідорова, І. В.; БУРКА О.А.

    2014-01-01

    It is proved that vitamin D plays a role in the prevention and treatment of many diseases such as immunodeficiency status, anemia, diabetes melitus, various pathological conditions of the liver, gastrointestinal tract, cardiovascular system, tuberculosis, tumors of breast, bowel, etc. Also it was found that vitamin D modulates reproductive processes in women particularly affects steroidogenesis of estradiol and progesterone, and anti-Mullerian hormone in healthy women.Therefore, at examining ...

  3. Stress and hormones

    Directory of Open Access Journals (Sweden)

    Salam Ranabir

    2011-01-01

    Full Text Available In the modern environment one is exposed to various stressful conditions. Stress can lead to changes in the serum level of many hormones including glucocorticoids, catecholamines, growth hormone and prolactin. Some of these changes are necessary for the fight or flight response to protect oneself. Some of these stressful responses can lead to endocrine disorders like Graves′ disease, gonadal dysfunction, psychosexual dwarfism and obesity. Stress can also alter the clinical status of many preexisting endocrine disorders such as precipitation of adrenal crisis and thyroid storm.

  4. Hormonal contraception and venous thromboembolism

    DEFF Research Database (Denmark)

    Lidegaard, Øjvind; Milsom, Ian; Geirsson, Reynir Tomas;

    2012-01-01

    New studies about the influence of hormonal contraception on the risk of venous thromboembolism (VTE) have been published.......New studies about the influence of hormonal contraception on the risk of venous thromboembolism (VTE) have been published....

  5. SHBG (Sex Hormone Binding Globulin)

    Science.gov (United States)

    ... as: Testosterone-estrogen Binding Globulin; TeBG Formal name: Sex Hormone Binding Globulin Related tests: Testosterone , Free Testosterone, ... I should know? How is it used? The sex hormone binding globulin (SHBG) test may be used ...

  6. Growth Hormone Deficiency in Children

    Science.gov (United States)

    ... which the body does not make enough growth hormone (GH). GH is made by the pituitary gland, a ... blood test checks levels of IGF-1, a hormone that reflects GH levels. • GH stimulation test. The child is given ...

  7. Thyroid hormone deiodination

    NARCIS (Netherlands)

    T.J. Visser (Ton)

    1980-01-01

    textabstractThe enzymatic deiodination of thyroid hormone is an important process since it concerns- among other things- the regulation of thyromimetic activity at the site of the target organ. To understand the mechanism of this regulation it is necessary to have a detailed knowledge of the mode of

  8. Hormone Therapy for Breast Cancer in Men

    Science.gov (United States)

    ... Topic Targeted therapy for breast cancer in men Hormone therapy for breast cancer in men Hormone therapy ... fatigue, and pain at the injection site. Luteinizing hormone-releasing hormone (LHRH) analogs and anti-androgens LHRH ...

  9. [Hormonal contraception in autoimmpne diseases].

    Science.gov (United States)

    Matyszkiewicz, Anna; Jach, Robert; Rajtar-Ciosek, Agnieszka; Basta, Tomasz

    2016-01-01

    The onset and the course of autoimmune diseases is influenced among other factors by the sex hormones. Hormonal contraception might affect the course of the autoimmune disease. The paper summarises the manner of save application of hormonal contraception in patients with autoimmune disease. PMID:27526427

  10. Growth Hormone: Use and Abuse

    Science.gov (United States)

    ... is huma n gr owth hormone? Human growth hormone (GH) is a substance that controls your body’s growth. ... prescribed for the FDA-approved conditions. In children, GH is used to treat • Growth hormone deficiency • Conditions that cause short stature (being shorter ...

  11. Study of anti - Mūllerian hormone in prediction of poor ovarian response%抗苗勒管激素对卵巢低反应预测价值的研究

    Institute of Scientific and Technical Information of China (English)

    朱英哲; 彭沙; 王青欣; 杨晓葵

    2012-01-01

    Objective: To evaluate anti - Mullerian hormone (AMH) in prediction of poor ovarian response to controlled ovarian hyperstimulation (COH) in assisted reproductive technology (ART). Methods; The level of bAMH protein secretion in serum was examined by ELISA in 181 cases of infertile woman with first cycle of in vitro fertilization (IVF) / intracytoplasmic sperm injection (IC-SI). The patients were classified into 2 groups including poor response (n =20, the number of oocytes retrieved ≤5 and normal response (n = 161, the number of oocytes retrieved > 5) according to ovarian response to COH. The relationship among bAMH level, age and the number of oocytes retrieved was analyzed by software engineering SPSS11.5. Results; ① A positive correlation was found between serum bAMH and the number of oocytes retrieved (r=0.709, P5) (P <0.05). ③ ROC curve analysis revealed that area under curve (AUC) was 0. 949 for bAMH. The sensitivity and specificity were 86. 3% and 90.0% , respectively when the cutoff value of bAMH was no higher than 3.65ng/ml. Conclusion; These results indicate that serum AMH represents the well endocrine marker to assess the poor ovarian response on COH in ART.%目的 探讨抗苗勒氏管激素(AMH)在辅助生殖技术(ART)中对控制性超排卵(COH)卵巢低反应的预测价值.方法 利用ELISA法对181例首次接受IVF/ICSI - ET治疗的不孕症患者基础血清AMH蛋白水平(bAMH)进行检测.根据患者COH卵巢反应将患者分为2组:卵巢低反应组20例(获卵数≤5个)和卵巢反应良好组161例(获卵数>5个).比较bAMH与年龄、获卵数、基础FSH等的相关性.结果 ①181例不孕症患者bAMH水平与获卵数呈显著正相关(r =0.709,P<0.05);②卵巢低反应组(获卵数≤5个)与反应良好组(获卵数>5个)的bAMH水平比较,差异有统计学意义(P<0.05);③受试者工作特征(ROC)曲线分析显示,bAMH水平的曲线下面积(AUC)为0.949.当bAMH截断值≤3.65ng

  12. Gastrointestinal hormones and their targets

    DEFF Research Database (Denmark)

    Rehfeld, Jens F.

    2014-01-01

    , paracrine, spermiocrine secretion etc.), so the same peptide may act as a blood-borne hormone, a neurotransmitter, a local growth factor, or a fertility factor. The molecular targets of each bioactive peptide are specific G-protein coupled receptors expressed in the cell membranes of different target cells......Gastrointestinal hormones are peptides released from endocrine cells and neurons in the digestive tract. More than 30 hormone genes are currently known to be expressed in the gastrointestinal tract, which makes the gut the largest hormone producing organ in the body. Modern biology makes...... it feasible to conceive the hormones under five headings: The structural homology groups a majority of the hormones into nine families, each of which is assumed to originate from one ancestral gene. The individual hormone gene often has multiple phenotypes due to alternative splicing, tandem organization...

  13. Biosimilar growth hormone.

    Science.gov (United States)

    Saenger, Paul

    2012-01-01

    As the first wave of biopharmaceuticals is expiring, biosimilars or follow-on -protein products (FOPP's) have emerged. Biosimilar drugs are cheaper than the originator/comparator drug. The regulatory foundation for these products is more advanced and better codified in Europe than in the US. Biosimilar soamtropin has been approved in both the US and Europe. The scientific viability of biosimilar drugs and especially growth hormone has been proven by several rigorously conducted clinical trials. Efficacy and safety data (growth rates, IGF-1 generation) for up to 7 y for pediatric indications measure up favorably to previously approved growth hormones which served as reference comparators. The Obama Administration appears to be committed to establish innovative pathways for the approval of biologics and biosimilars in the US. The cost savings in health care expenditures will be substantial as the global sales of biologics have reached $ 93 billion in 2009.

  14. Hormones in pregnancy

    Directory of Open Access Journals (Sweden)

    Pratap Kumar

    2012-01-01

    Full Text Available The endocrinology of human pregnancy involves endocrine and metabolic changes that result from physiological alterations at the boundary between mother and fetus. Progesterone and oestrogen have a great role along with other hormones. The controversies of use of progestogen and others are discussed in this chapter. Progesterone has been shown to stimulate the secretion of Th2 and reduces the secretion of Th1 cytokines which maintains pregnancy. Supportive care in early pregnancy is associated with a significant beneficial effect on pregnancy outcome. Prophylactic hormonal supplementation can be recommended for all assisted reproduction techniques cycles. Preterm labor can be prevented by the use of progestogen. The route of administration plays an important role in the drug′s safety and efficacy profile in different trimesters of pregnancy. Thyroid disorders have a great impact on pregnancy outcome and needs to be monitored and treated accordingly. Method of locating review: Pubmed, scopus

  15. Hormones in pregnancy

    OpenAIRE

    Pratap Kumar; Navneet Magon

    2012-01-01

    The endocrinology of human pregnancy involves endocrine and metabolic changes that result from physiological alterations at the boundary between mother and fetus. Progesterone and oestrogen have a great role along with other hormones. The controversies of use of progestogen and others are discussed in this chapter. Progesterone has been shown to stimulate the secretion of Th2 and reduces the secretion of Th1 cytokines which maintains pregnancy. Supportive care in early pregnancy is associated...

  16. The wound hormone jasmonate

    OpenAIRE

    Koo, Abraham J.K.; Howe, Gregg A.

    2009-01-01

    Plant tissues are highly vulnerable to injury by herbivores, pathogens, mechanical stress, and other environmental insults. Optimal plant fitness in the face of these threats relies on complex signal transduction networks that link damage-associated signals to appropriate changes in metabolism, growth, and development. Many of these wound-induced adaptive responses are triggered by de novo synthesis of the plant hormone jasmonate (JA). Recent studies provide evidence that JA mediates systemic...

  17. 抗苗勒激素对体外受精-胚胎移植结局的预测作用%Anti-müllerian Hormone Has an Predictive Effect on the Outcome of In Vitro Fertilization-Embryo Transfer

    Institute of Scientific and Technical Information of China (English)

    程萍; 张丽萍; 刘永珍

    2012-01-01

    Objective To study the relationships between anti - miillerian hormone in serum and follicular fluid and embryonic quality and successful pregnancy. Methods Enzyme-linked immunosorbent assay (ELJSA) was used to detect anti -mullerian hormone(AMH) levels in serum on the day of oocyte pick-up(OPU),embryo transfer(ET) ,ET + 3 and in follicular Quid on the day of OPU in 48 patients(48 cycles) undergoing in vitro fertilization-embryo transfer(IVF-ET). Results (l)The AMH concentrations in follicular fluid of patients whose high-quality embryonic ratio is no less than 0.5 are significantly higher than those whose high-quality embryonic ratio is less than 0.5 (P 0.05, respectively). (3) However,the AMH concentration in follicular fluid of pregnant group are markedly higher than non-pregnant group( P < 0. 05). Conclusion The AMH levels of every stage after OPU in serum can not predict the outcome of IVF-ET. The AMH concentration in follicular fluid is a factor that could be used to predict the outcome of IVF-ET. It may lead to the change of embryonic quality by altering follicular microenvironment locally and then affecting the outcome of IVF-ET.%目的:研究行体外受精-胚胎移植(IVF-ET)的患者血清及卵泡液中抗苗勒激素(AMH)与胚胎质量、妊娠结局之间的关系.方法:酶联免疫吸附实验(ELISA)检测48例行IVF-ET患者(48个周期)取卵日、胚胎移植日和胚胎移植后3d血清中AMH水平及取卵日卵泡液中AMH水平,了解其与胚胎质量、妊娠结局之间的关系.结果:(1)高质量胚胎比≥0.5组卵泡液AMH水平较高质量胚胎比<0.5组显著升高(P<0.05);(2)在妊娠组与非妊娠组之间,取卵日、胚胎移植日和胚胎移植后3d血清中AMH水平无差异(P>0.05);(3)妊娠组卵泡液AMH水平明显高于非妊娠组(P<0.05).结论:取卵后各个时期的血清AMH水平不能预测IVF-ET妊娠结局,取卵日卵泡液中AMH水平可以作为IVF-ET妊娠结局的预测因子.卵泡液

  18. Testes and brain gene expression in precocious male and adult maturing Atlantic salmon (Salmo salar

    Directory of Open Access Journals (Sweden)

    Houeix Benoit

    2010-03-01

    Full Text Available Abstract Background The male Atlantic salmon generally matures in fresh water upon returning after one or several years at sea. Some fast-growing male parr develop an alternative life strategy where they sexually mature before migrating to the oceans. These so called 'precocious' parr or 'sneakers' can successfully fertilise adult female eggs and so perpetuate their line. We have used a custom-built cDNA microarray to investigate gene expression changes occurring in the salmon gonad and brain associated with precocious maturation. The microarray has been populated with genes selected specifically for involvement in sexual maturation (precocious and adult and in the parr-smolt transformation. Results Immature and mature parr collected from a hatchery-reared stock in January were significantly different in weight, length and condition factor. Changes in brain expression were small - never more than 2-fold on the microarray, and down-regulation of genes was much more pronounced than up-regulation. Significantly changing genes included isotocin, vasotocin, cathepsin D, anamorsin and apolipoprotein E. Much greater changes in expression were seen in the testes. Among those genes in the testis with the most significant changes in expression were anti-Mullerian hormone, collagen 1A, and zinc finger protein (Zic1, which were down-regulated in precocity and apolipoproteins E and C-1, lipoprotein lipase and anti-leukoproteinase precursor which were up-regulated in precocity. Expression changes of several genes were confirmed in individual fish by quantitative PCR and several genes (anti-Mullerian hormone, collagen 1A, beta-globin and guanine nucleotide binding protein (G protein beta polypeptide 2-like 1 (GNB2L1 were also examined in adult maturing testes. Down-regulation of anti-Mullerian hormone was judged to be greater than 160-fold for precocious males and greater than 230-fold for November adult testes in comparison to July testes by this method. For

  19. A nonpeptidyl growth hormone secretagogue.

    Science.gov (United States)

    Smith, R G; Cheng, K; Schoen, W R; Pong, S S; Hickey, G; Jacks, T; Butler, B; Chan, W W; Chaung, L Y; Judith, F

    1993-06-11

    A nonpeptidyl secretagogue for growth hormone of the structure 3-amino-3-methyl-N-(2,3,4,5-tetrahydro-2-oxo-1-([2'-(1H-tetrazol-5 -yl) (1,1'-biphenyl)-4-yl]methyl)-1H-1-benzazepin-3(R)-yl)-butanamid e (L-692,429) has been identified. L-692,429 synergizes with the natural growth hormone secretagogue growth hormone-releasing hormone and acts through an alternative signal transduction pathway. The mechanism of action of L-692,429 and studies with peptidyl and nonpeptidyl antagonists suggest that this molecule is a mimic of the growth hormone-releasing hexapeptide His-D-Trp-Ala-Trp-D-Phe-Lys-NH2 (GHRP-6). L-692,429 is an example of a nonpeptidyl specific secretagogue for growth hormone.

  20. Quo vadis plant hormone analysis?

    OpenAIRE

    Tarkowská, D. (Danuše); Novák, O. (Ondřej); Floková, K. (Kristýna); Tarkowski, P.; Turečková, V. (Veronika); Grúz, J. (Jiří); Rolčík, J. (Jakub); Strnad, M.

    2014-01-01

    Plant hormones act as chemical messengers in the regulation of myriads of physiological processes that occur in plants. To date, nine groups of plant hormones have been identified and more will probably be discovered. Furthermore, members of each group may participate in the regulation of physiological responses in planta both alone and in concert with members of either the same group or other groups. The ideal way to study biochemical processes involving these signalling molecules is 'hormon...

  1. Mammalian sex hormones in plants

    OpenAIRE

    Andrzej Skoczowski; Anna Janeczko

    2011-01-01

    The occurrence of mammalian sex hormones and their physiological role in plants is reviewed. These hormones, such as 17β-estradiol, androsterone, testosterone or progesterone, were present in 60-80% of the plant species investigated. Enzymes responsible for their biosynthesis and conversion were also found in plants. Treatment of the plants with sex hormones or their precursors influenced plant development: cell divisions, root and shoot growth, embryo growth, flowering, pollen tube ...

  2. Hormones and Borderline Personality Features

    OpenAIRE

    Evardone, Milagros; Alexander, Gerianne M.; Morey, Leslie C.

    2008-01-01

    Borderline personality is diagnosed in clinical settings three times more often in women than in men, and symptom severity in women appears sensitive to circulating sex steroid levels. In non-human mammals, prenatal hormones contribute to the development of sex-linked behavior and their responsiveness to postnatal hormones. Therefore, this study examined the hypothesis that prenatal hormones may influence the development of borderline personality traits by measuring a marker of perinatal andr...

  3. Growth hormone and aging

    OpenAIRE

    Bartke, Andrzej; Brown-Borg, Holly; Kinney, Beth; Mattison, Julie; Wright, Chris; Hauck, Steven; Coschigano, Karen; Kopchick, John

    2000-01-01

    The potential usefulness of growth hormone (GH) as an anti-aging therapy is of considerable current interest. Secretion of GH normally declines during aging and administration of GH can reverse age-related changes in body composition. However, mutant dwarf mice with congenital GH deficiency and GH resistant GH-R-KO mice live much longer than their normal siblings, while a pathological elevation of GH levels reduces life expectancy in both mice and men. We propose that the actions of GH on gro...

  4. [Hormone replacement therapy--growth hormone, melatonin, DHEA and sex hormones].

    Science.gov (United States)

    Fukai, Shiho; Akishita, Masahiro

    2009-07-01

    The ability to maintain active and independent living as long as possible is crucial for the healthy longevity. Hormones responsible for some of the manifestations associated with aging are growth hormone, insulin-like growth factor-1 (IGF-1), melatonin, dehydroepiandrosterone (DHEA), sex hormones and thyroid hormones. These hormonal changes are associated with changes in body composition, visceral obesity, muscle weakness, osteoporosis, urinary incontinence, loss of cognitive functioning, reduction in well being, depression, as well as sexual dysfunction. With the prolongation of life expectancy, both men and women today live the latter third life with endocrine deficiencies. Hormone replacement therapy may alleviate the debilitating conditions of secondary partial endocrine deficiencies by preventing or delaying some aspects of aging.

  5. Alternatives of menopausal hormone therapy

    OpenAIRE

    Kutlešić Ranko M.; Popović Jasmina; Stefanović Milan; Vukomanović Predrag; Lukić Bojan; Lilić Goran

    2016-01-01

    Introduction. It has been generally accepted that the benefits of menopausal hormone therapy outweigh the risks, but there are still some concerns about the administration of menopausal hormone therapy, which has introduced alternative treatments. Pharmacological Alternatives. Central alpha-2 agonist clonidine is only marginally more effective than placebo, and significantly less effective than estrogen. Antiepileptic drug gabapentin reduces hot flashes; ho...

  6. Hormonal control of inflammatory responses

    Directory of Open Access Journals (Sweden)

    J. Garcia-Leme

    1993-01-01

    Full Text Available Almost any stage of inflammatory and immunological responses is affected by hormone actions. This provides the basis for the suggestion that hormones act as modulators of the host reaction against trauma and infection. Specific hormone receptors are detected in the reactive structures in inflamed areas and binding of hormone molecules to such receptors results in the generation of signals that influence cell functions relevant for the development of inflammatory responses. Diversity of hormonal functions accounts for recognized pro- and anti-inflammatory effects exerted by these substances. Most hormone systems are capable of influencing inflammatory events. Insulin and glucocorticoids, however, exert direct regulatory effects at concentrations usually found in plasma. Insulin is endowed with facilitatory actions on vascular reactivity to inflammatory mediators and inflammatory cell functions. Increased concentrations of circulating glucocorticoids at the early stages of inflammation results in downregulation of inflammatory responses. Oestrogens markedly reduce the response to injury in a variety of experimental models. Glucagon and thyroid hormones exert indirect anti-inflammatory effects mediated by the activity of the adrenal cortex. Accordingly, inflammation is not only merely a local response, but a hormone-controlled process.

  7. Measurement of the incretin hormones

    DEFF Research Database (Denmark)

    Kuhre, Rune Ehrenreich; Wewer Albrechtsen, Nicolai Jacob; Hartmann, Bolette;

    2015-01-01

    concentrations of GLP-1 and GIP is often an important endpoint in both clinical and preclinical studies and, therefore, accurate measurement of these hormones is important. Here, we provide an overview of current approaches for the measurement of the incretin hormones, with particular focus on immunological...

  8. Recent advances in hormonal contraception

    OpenAIRE

    Li, HW Raymond; Richard A. Anderson

    2010-01-01

    This report reviews some of the new studies regarding new hormonal contraceptive formulations (e.g., Yaz, Qlaira®, extended-cycle or continuous combined contraceptives, subcutaneous depot medroxyprogesterone acetate, and ulipristal acetate as an emergency contraceptive). Recent data on the relationship between hormonal contraceptive use and bone health are also reviewed. © 2010 Medicine Reports Ltd.

  9. Sex Hormones and Ischemic Stroke

    DEFF Research Database (Denmark)

    Holmegard, Haya N; Nordestgaard, Børge G; Jensen, Gorm B;

    2016-01-01

    CONTEXT AND OBJECTIVE: Whether endogenous sex hormones are associated with ischemic stroke (IS) is unclear. We tested the hypothesis that extreme concentrations of endogenous sex hormones are associated with risk of IS in the general population. DESIGN, SETTING, AND PARTICIPANTS: Adult men (n...

  10. Types of Cancer Treatment: Hormone Therapy

    Science.gov (United States)

    Describes how hormone therapy slows or stops the growth of breast and prostate cancers that use hormones to grow. Includes information about the types of hormone therapy and side effects that may happen.

  11. Genetics Home Reference: combined pituitary hormone deficiency

    Science.gov (United States)

    ... Genetics Home Health Conditions combined pituitary hormone deficiency combined pituitary hormone deficiency Enable Javascript to view the ... boxes. Print All Open All Close All Description Combined pituitary hormone deficiency is a condition that causes ...

  12. Leptin: a multifunctional hormone

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    Leptin is the protein product encoded by the obese (ob)gene. It is a circulating hormone produced primarily by the adipose tissue. ob/ob mice with mutations of the gene encoding leptin become morbidly obese, infertile, hyperphagic, hypothermic,and diabetic. Since the cloning of leptin in 1994, our knowledge in body weight regulation and the role played by leptin has increased substantially. We now know that leptin signals through its receptor, OB-R, which is a member of the cytokine receptor superfamily. Leptin serves as an adiposity signal to inform the brain the adipose tissue mass in a negative feedback loop regulating food intake and energy expenditure. Leptin also plays important roles in angiogenesis, immune function, fertility, and bone formation. Humans with mutations in the gene encoding leptin are also morbidly obese and respond to leptin treatment,demonstrating that enhancing or inhibiting leptin's activities in vivo may have potential therapeutic benefits.

  13. Genetics Home Reference: isolated growth hormone deficiency

    Science.gov (United States)

    ... deficiency dwarfism, pituitary growth hormone deficiency dwarfism isolated GH deficiency isolated HGH deficiency isolated human growth hormone deficiency isolated somatotropin deficiency isolated somatotropin deficiency disorder ...

  14. Genotoxic potential of nonsteroidal hormones

    Directory of Open Access Journals (Sweden)

    Topalović Dijana

    2015-01-01

    Full Text Available Hormones are cellular products involved in the regulation of a large number of processes in living systems, and which by their actions affect the growth, function and metabolism of cells. Considering that hormones are compounds normally present in the organism, it is important to determine if they can, under certain circumstances, lead to genetic changes in the hereditary material. Numerous experimental studies in vitro and in vivo in different systems, from bacteria to mammals, dealt with the mutagenic and genotoxic effects of hormones. This work presents an overview of the research on genotoxic effects of non­steroidal hormones, although possible changes of genetic material under their influence have not still been known enough, and moreover, investigations on their genotoxic influence have given conflicting results. The study results show that mechanisms of genotoxic effect of nonsteroidal hormones are manifested through the increase of oxidative stress by arising reactive oxygen species. A common mechanism of ROS occurence in thyroid hormones and catecholamines is through metabolic oxidation of their phenolic groups. Manifestation of insulin genotoxic effect is based on production of ROS by activation of NADPH isophorms, while testing oxytocin showed absence of genotoxic effect. Considering that the investigations on genotoxicity of nonsteroidal hormones demonstrated both positive and negative results, the explanation of this discordance involve limitations of test systems themselves, different cell types or biological species used in the experiments, different level of reactivity in vitro and in vivo, as well as possible variations in a tissue-specific expression. Integrated, the provided data contribute to better understanding of genotoxic effect of nonsteroidal hormones and point out to the role and mode of action of these hormones in the process of occurring of effects caused by oxidative stress. [Projekat Ministarstva nauke Republike

  15. Specific involvement of gonadal hormones in the functional maturation of growth hormone releasing hormone (GHRH) neurons.

    Science.gov (United States)

    Gouty-Colomer, Laurie-Anne; Méry, Pierre-François; Storme, Emilie; Gavois, Elodie; Robinson, Iain C; Guérineau, Nathalie C; Mollard, Patrice; Desarménien, Michel G

    2010-12-01

    Growth hormone (GH) is the key hormone involved in the regulation of growth and metabolism, two functions that are highly modulated during infancy. GH secretion, controlled mainly by GH releasing hormone (GHRH), has a characteristic pattern during postnatal development that results in peaks of blood concentration at birth and puberty. A detailed knowledge of the electrophysiology of the GHRH neurons is necessary to understand the mechanisms regulating postnatal GH secretion. Here, we describe the unique postnatal development of the electrophysiological properties of GHRH neurons and their regulation by gonadal hormones. Using GHRH-eGFP mice, we demonstrate that already at birth, GHRH neurons receive numerous synaptic inputs and fire large and fast action potentials (APs), consistent with effective GH secretion. Concomitant with the GH secretion peak occurring at puberty, these neurons display modifications of synaptic input properties, decrease in AP duration, and increase in a transient voltage-dependant potassium current. Furthermore, the modulation of both the AP duration and voltage-dependent potassium current are specifically controlled by gonadal hormones because gonadectomy prevented the maturation of these active properties and hormonal treatment restored it. Thus, GHRH neurons undergo specific developmental modulations of their electrical properties over the first six postnatal weeks, in accordance with hormonal demand. Our results highlight the importance of the interaction between the somatotrope and gonadotrope axes during the establishment of adapted neuroendocrine functions.

  16. Revisiting Thyroid Hormones in Schizophrenia

    Directory of Open Access Journals (Sweden)

    Nadine Correia Santos

    2012-01-01

    Full Text Available Thyroid hormones are crucial during development and in the adult brain. Of interest, fluctuations in the levels of thyroid hormones at various times during development and throughout life can impact on psychiatric disease manifestation and response to treatment. Here we review research on thyroid function assessment in schizophrenia, relating interrelations between the pituitary-thyroid axis and major neurosignaling systems involved in schizophrenia’s pathophysiology. These include the serotonergic, dopaminergic, glutamatergic, and GABAergic networks, as well as myelination and inflammatory processes. The available evidence supports that thyroid hormones deregulation is a common feature in schizophrenia and that the implications of thyroid hormones homeostasis in the fine-tuning of crucial brain networks warrants further research.

  17. Sex Hormones and Immune Dimorphism

    Science.gov (United States)

    Bhatia, Aruna; Sekhon, Harmandeep Kaur; Kaur, Gurpreet

    2014-01-01

    The functioning of the immune system of the body is regulated by many factors. The abnormal regulation of the immune system may result in some pathological conditions. Sex hormones of reproductive system are one of the major factors that regulate immune system due to the presence of hormone receptors on immune cells. The interaction of sex hormones and immune cells through the receptors on these cells effect the release of cytokines which determines the proliferation, differentiation, and maturation of different types of immunocytes and as a result the outcome of inflammatory or autoimmune diseases. The different regulations of sex hormones in both sexes result in immune dimorphism. In this review article the mechanism of regulation of immune system in different sexes and its impact are discussed. PMID:25478584

  18. Sex Hormones and Immune Dimorphism

    Directory of Open Access Journals (Sweden)

    Aruna Bhatia

    2014-01-01

    Full Text Available The functioning of the immune system of the body is regulated by many factors. The abnormal regulation of the immune system may result in some pathological conditions. Sex hormones of reproductive system are one of the major factors that regulate immune system due to the presence of hormone receptors on immune cells. The interaction of sex hormones and immune cells through the receptors on these cells effect the release of cytokines which determines the proliferation, differentiation, and maturation of different types of immunocytes and as a result the outcome of inflammatory or autoimmune diseases. The different regulations of sex hormones in both sexes result in immune dimorphism. In this review article the mechanism of regulation of immune system in different sexes and its impact are discussed.

  19. Hormone Therapy for Prostate Cancer

    Science.gov (United States)

    ... agonists , which are sometimes called LHRH analogs, are synthetic proteins that are structurally similar to LHRH and ... gland to stop producing luteinizing hormone, which prevents testosterone from being produced. Treatment with an LHRH agonist ...

  20. Stress hormones and physical activity

    OpenAIRE

    Editorial Office

    1991-01-01

    Hormone secretion during physical activity of specific duration and intensity is part of the stress response. In a study to investigate the secretion of ß-endorphin, leucine enkephalin and other recognised stress hormones during physical exercise, blood samples were taken from fourteen (14) healthy, male athletes who competed in a 21 km roadrace. Blood samples were collected before and after completion of the race. This study shows that ß-endorphin/ß-lipotropin, leucine enkephalin, prolact...

  1. Fatigue and cognition - hormonal perspectives

    OpenAIRE

    Möller, Marika

    2013-01-01

    Fatigue is a common complaint and considered a very challenging symptom to cope with in many different medical diseases. The assessment of fatigue is bound up with the problems of both conceptualization and definition. In addition, few studies have investigated suitable neuropsychological tests to examine fatigue and its consequences. This thesis evaluates whether neuropsychological tests can elicit cognitive fatigue. It also investigates whether specific hormones and hormon...

  2. Does growth hormone cause cancer?

    OpenAIRE

    Jenkins, P.J.; Mukherjee, A.; Shalet, S. M.

    2006-01-01

    KEYWORDS - CLASSIFICATION: adverse effects;Acromegaly;Adult;Animals;cancer epidemiology;complications;Child;Child Development;Colorectal Neoplasms;deficiency;epidemiology;etiology;Evaluation;Growth Hormone;Human Growth Hormone;Humans;Insulin-Like Growth Factor I;mechanisms of carcinogenesis;Neoplasm Recurrence,Local;Neoplasms;Neoplasms,Multiple Primary;physiology;physiopathology;Risk Factors;secretion;therapy. The ability of GH, via its mediator peptide IGF-1, to influence regulation of ce...

  3. Revisiting Thyroid Hormones in Schizophrenia

    OpenAIRE

    Nadine Correia Santos; Patrício Costa; Dina Ruano; António Macedo; Maria João Soares; José Valente; Ana Telma Pereira; Maria Helena Azevedo; Joana Almeida Palha

    2012-01-01

    Thyroid hormones are crucial during development and in the adult brain. Of interest, fluctuations in the levels of thyroid hormones at various times during development and throughout life can impact on psychiatric disease manifestation and response to treatment. Here we review research on thyroid function assessment in schizophrenia, relating interrelations between the pituitary-thyroid axis and major neurosignaling systems involved in schizophrenia’s pathophysiology. These include the ser...

  4. Plant hormone receptors: new perceptions

    OpenAIRE

    Spartz, Angela K.; William M Gray

    2008-01-01

    Plant growth and development require the integration of a variety of environmental and endogenous signals that, together with the intrinsic genetic program, determine plant form. Central to this process are several growth regulators known as plant hormones or phytohormones. Despite decades of study, only recently have receptors for several of these hormones been identified, revealing novel mechanisms for perceiving chemical signals and providing plant biologists with a much clearer picture of...

  5. History of growth hormone therapy

    OpenAIRE

    Vageesh S Ayyar

    2011-01-01

    Although the importance of the pituitary gland for growth was recognized in late 19 th century, Growth hormone (GH) therapy was made available for severely GH-deficient children and adolescents only in late 1950s. Use of GH for other conditions was limited because of the limited supply of human pituitary-derived hormone. With unlimited availability of recombinant human GH (rhGH), the scenario of GH treatment has been changed enormously. Currently there is ever increasing list of indications o...

  6. Short Stature and Growth Hormone Deficiency

    OpenAIRE

    Matemi, Sezer

    1994-01-01

    This paper summarizes the importance of measurements of height and weight in normal children and emphasizes the role of growth increments for the diagnosis of short stature Causes of short stature methods for diagnosis of GH hormone deficiency actions of growth hormone treatment of growth hormone deficiency and doses for biosynthetic GH treatment are described Key words: Short Stature Growth Hormone

  7. Hormone therapy and ovarian borderline tumors

    DEFF Research Database (Denmark)

    Mørch, Lina Steinrud; Løkkegaard, Ellen; Andreasen, Anne Helms;

    2012-01-01

    Little is known about the influence of postmenopausal hormone therapy on the risk of ovarian borderline tumors. We aimed at assessing the influence of different hormone therapies on this risk.......Little is known about the influence of postmenopausal hormone therapy on the risk of ovarian borderline tumors. We aimed at assessing the influence of different hormone therapies on this risk....

  8. Growth Hormone and Endocrinopathies

    International Nuclear Information System (INIS)

    This is an analysis of 39 patients studied at the Yonsei Medical Center from January, 1976 to March 1979. Of these 35 patient were suspected of having hypothalamic insufficiency and subjected to the L-Dopa stimulation test to observe growth hormone secretary function while four acromegaly patient received the glucose loading test and L-Dopa stimulation test. The results are as follows: 1) The basal level of GH in the various disease was as follows: a) The basal level was lower than the control level but was not statistically significant b) In diabetes the mean value tended to higher than the control level but was not significant statistically c) In all four acromegaly patients the GH level was significantly higher than the control level 2) Of 13 patients with diabetes, nine had diabetic retinopathy, and of those nine, six showed increased L-Dopa response. However, of the four non retinopathic DM patients, only one showed increased response to L-Dopa. 3) Two patients out of ten with Sheehan's syndrome responded to L-Dopa stimulation. 4) One Patient of eight with pituitary chromophobe adenoma responded to L-Dopa stimulation. 5) Four acromegaly patients revealed 3 acidophilic adenoma and one chromophobe adenoma histologically. Of patients receiving the L-Dopa stimulation test. Two showed a paradoxical response. Two patients who received the glucose loading test showed suppressed response. 6) Of two craniopharyngioma patients, one showed increased GH response after L-Dopa stimulation. Increased response of GH after L-Dopa stimulation was seen in one two craniopharyngioma patients and also in one of two patients with short structure.

  9. Thyroid Hormone Deiodinases and Cancer

    Directory of Open Access Journals (Sweden)

    Antonio eBianco

    2012-06-01

    Full Text Available Deiodinases constitute a group of thioredoxin-containing selenoenzymes that play an important function in thyroid hormone homeostasis and control of thyroid hormone action. There are three known deiodinases: D1 and D2 activate the pro-hormone thyroxine (T4 to T3, the most active form of thyroid hormone, while D3 inactivates thyroid hormone and terminates T3 action. A number of studies indicate that deiodinase expression is altered in several types of cancers, suggesting that (i they may represent a useful cancer marker and/or (ii could play a role in modulating cell proliferation - in different settings thyroid hormone modulates cell proliferation. For example, although D2 is minimally expressed in human and rodent skeletal muscle, its expression level in rhabdomyosarcoma (RMS-13 cells is 3-4 fold higher. In basal cell carcinoma (BCC cells, sonic hedgehog (Shh-induced cell proliferation is accompanied by induction of D3 and inactivation of D2. Interestingly a 5-fold reduction in the growth of BCC in nude mice was observed if D3 expression was knocked down. A decrease in D1 activity has been described in renal clear cell carcinoma, primary liver cancer, lung cancer, and some pituitary tumors, while in breast cancer cells and tissue there is an increase in D1 activity. Furthermore D1 mRNA and activity were found to be decreased in papillary thyroid cancer while D1 and D2 activities were significantly higher in follicular thyroid cancer tissue, in follicular adenoma and in anaplastic thyroid cancer. It is conceivable that understanding how deiodinase dysregulation in tumor cells affect thyroid hormone signaling and possibly interfere with tumor progression could lead to new antineoplastic approaches.

  10. Alternatives of menopausal hormone therapy

    Directory of Open Access Journals (Sweden)

    Kutlešić Ranko M.

    2016-01-01

    Full Text Available Introduction. It has been generally accepted that the benefits of menopausal hormone therapy outweigh the risks, but there are still some concerns about the administration of menopausal hormone therapy, which has introduced alternative treatments. Pharmacological Alternatives. Central alpha-2 agonist clonidine is only marginally more effective than placebo, and significantly less effective than estrogen. Antiepileptic drug gabapentin reduces hot flashes; however, it is less effective than estrogen. Selective serotonin reuptake inhibitors (paroxetine and fluoxetine and selective noradrenaline reuptake inhibitors (venlafaxine reduce vasomotor symptoms and improve depression, anxiety and sleep. Results of studies about dehydroepiandrosterone effects on menopausal symptoms are inconsistent and additional investigations are needed. Non-Pharmacological Alternatives. Stellatum ganglion blockade is a successful treatment for reducing vasomotor symptoms in patients with contraindications for menopausal hormone therapy. Efficacy of acupuncture, homeopathy and reflexology should be proved by adequate studies. Phytoestrogens could reduce vasomotor symptoms but to a lesser extent than conventional menopausal hormone therapy. However, they have not been proved yet to provide cardiovascular protection and prevention of osteoporosis, nor they could be recommended instead of traditional menopausal hormone therapy. There is a concern about their undesirable effects. Adequate diet, unchanging body weight within ideal values and adequate physical activities have beneficial long-term effects, first of all on preservation of bone density. Alternatives for Atrophic Changes of Vaginal Epithelium. Menopausal symptoms resulting from vaginal atrophy could be resolved by use of hydrophilic preparations, lubricants and topical lidocaine cream or 4% lidocaine water solution for dyspareunia. Conclusion. If there are contrain­dications to menopausal hormone therapy or

  11. Ovarian reserve tests

    Directory of Open Access Journals (Sweden)

    Padma Rekha Jirge

    2011-01-01

    Full Text Available Ovarian reserve plays a crucial role in achieving pregnancy following any treatment in subfertile women. The estimation of ovarian reserve is routinely performed through various ovarian reserve tests (ORTs in an effort to predict the response and outcome in couples prior to In Vitro Fertilization and counsel them. Most widely used tests are basal follicle stimulating hormone and anti-Mullerian hormone and antral follicle count. The role of ORTs in our routine practice is discussed in this article. A MEDLINE search was done to identify suitable articles for review.

  12. Hormone therapy and ovarian cancer

    DEFF Research Database (Denmark)

    Mørch, Lina Steinrud; Løkkegaard, Ellen; Andreasen, Anne Helms;

    2009-01-01

    CONTEXT: Studies have suggested an increased risk of ovarian cancer among women taking postmenopausal hormone therapy. Data are sparse on the differential effects of formulations, regimens, and routes of administration. OBJECTIVE: To assess risk of ovarian cancer in perimenopausal and postmenopau......CONTEXT: Studies have suggested an increased risk of ovarian cancer among women taking postmenopausal hormone therapy. Data are sparse on the differential effects of formulations, regimens, and routes of administration. OBJECTIVE: To assess risk of ovarian cancer in perimenopausal...... and postmenopausal women receiving different hormone therapies. DESIGN AND SETTING: Nationwide prospective cohort study including all Danish women aged 50 through 79 years from 1995 through 2005 through individual linkage to Danish national registers. Redeemed prescription data from the National Register...... bands included hormone exposures as time-dependent covariates. PARTICIPANTS: A total of 909,946 women without hormone-sensitive cancer or bilateral oophorectomy. MAIN OUTCOME MEASURE: Ovarian cancer. RESULTS: In an average of 8.0 years of follow-up (7.3 million women-years), 3068 incident ovarian...

  13. Radioimmunological and clinical studies with luteinizing hormone releasing hormone (LRH)

    International Nuclear Information System (INIS)

    Radioimmunoassay for Luteinizing Hormone Releasing Hormone (LRH) has been established, tested and applied. Optimal conditions for the performance with regards to incubation time, incubation temperature, concentration of antiserum and radiolabelled LRH have been established. The specificity of the LRH immunoassay was investigated. Problems with direct measurement of LRH in plasmas of radioimmunoassay are encountered. The LRH distribution in various tissues of the rat are investigated. By means of a system for continuous monitoring of LH and FSH in women the lowest effective dose of LRH causing a significant release of LH and FSH could be established. (Auth.)

  14. Gastrointestinal hormone research - with a Scandinavian annotation

    DEFF Research Database (Denmark)

    Rehfeld, Jens F

    2015-01-01

    as a blood-borne hormone, a neurotransmitter, a local growth factor or a fertility factor. The targets of gastrointestinal hormones are specific G-protein-coupled receptors that are expressed in the cell membranes also outside the digestive tract. Thus, gut hormones not only regulate digestive functions......Gastrointestinal hormones are peptides released from neuroendocrine cells in the digestive tract. More than 30 hormone genes are currently known to be expressed in the gut, which makes it the largest hormone-producing organ in the body. Modern biology makes it feasible to conceive the hormones...... under five headings: The structural homology groups a majority of the hormones into nine families, each of which is assumed to originate from one ancestral gene. The individual hormone gene often has multiple phenotypes due to alternative splicing, tandem organization or differentiated posttranslational...

  15. Pathology of growth hormone excess.

    Science.gov (United States)

    Kovacs, K

    1988-09-01

    This paper briefly reviews the pathology of growth hormone excess. Prolonged oversecretion of growth hormone is associated with elevated serum growth hormone as well as somatomedian C levels and the clinical signs and symptoms of acromegaly or gigantism. Morphologic studies, including immunohistochemistry and electron microscopy, revealed that several distinct morphologic lesions can be present in the pituitary gland of patients with acromegaly or gigantism. Although substantial progress has been achieved during the last two decades, more work is required to correlate the morphologic features of adenoma cells with their biologic behavior. We feel that the future can be viewed with optimism and further exciting results can be expected by the interaction of pathologists, clinical endocrinologists and basic scientists. PMID:3070506

  16. Stress hormones and physical activity

    Directory of Open Access Journals (Sweden)

    Editorial Office

    1991-07-01

    Full Text Available Hormone secretion during physical activity of specific duration and intensity is part of the stress response. In a study to investigate the secretion of ß-endorphin, leucine enkephalin and other recognised stress hormones during physical exercise, blood samples were taken from fourteen (14 healthy, male athletes who competed in a 21 km roadrace. Blood samples were collected before and after completion of the race. This study shows that ß-endorphin/ß-lipotropin, leucine enkephalin, prolactin, and melatonin may be classified as stress hormones in physical activity of duration 80 to 120 minutes and intensity exceeding 75%-V0₂max. Widespread intra-individual variation in serum cortisol concentrations prevent definite conclusion. The un­expected increase in serum testosterone levels warrants further research.

  17. Growth Hormone and Cerebral Amyloidosis.

    Science.gov (United States)

    Benvenga, S; Guarneri, F

    2016-08-01

    Great interest has recently been focused on a paper reporting characteristic deposits of amyloid-β protein associated with Alzheimer's disease in brains of adults who died of Creutzfeldt-Jakob disease. As they had contracted such disease after treatment with prion-contaminated human growth hormone extracted from cadaver-derived pituitaries, the authors have suggested that interhuman transmission of Alzheimer's disease had occurred. Our previous research led us to find that amyloid-forming peptides share amino acid sequence homology, summarized by a motif. Here, we probed the amino acid sequence of human growth hormone for such a motif, and found that 2 segments fit the motif and are potentially amyloid-forming. This finding was confirmed by Aggrescan, another well-known software for the prediction of amyloidogenic peptides. Our results, taken together with data from the literature that are missing in the aforementioned paper and associated commentaries, minimize the contagious nature of the iatrogenically-acquired coexistence of Creutzfeldt-Jakob disease and Alzheimer's disease. In particular, the above mentioned paper misses literature data on intratumoral amyloidosis in growth hormone- and prolactin-secreting adenomas, tumors relatively frequent in adults, which are often silent. It cannot be excluded that some pituitaries used to extract growth hormone contained clinically silent microadenomas, a fraction of which containing amyloid deposits, and patients might had received a fraction of growth hormone (with or without prolactin) that already was an amyloid seed. The intrinsic amyloidogenicity of growth hormone, in the presence of contaminating prion protein (and perhaps prolactin as well) and amyloid-β contained in some cadavers' pituitaries, may have led to the observed co-occurring of Creutzfeldt-Jakob disease and Alzheimer's disease. PMID:27214308

  18. Hormonal interaction in diabetic pregnancy

    International Nuclear Information System (INIS)

    Serum glucose, human placental lactogen (HPL), prolactin (PRL), estradiol (E2), progesterone (P), cortisol and human growth hormone (HGH) were determined in nondiabetic (19 cases) and diabetic (19 cases) pregnant women during the 32nd and 36th week of gestation. Significant elevation of HPL, PRL, HGH and cortisol was found in the diabetic pregnant women during the 32nd week while E2 and P were not significantly changed from the corresponding levels in the nondiabetic group. One can conclude that the changes in the hormonal pattern during gestation may induce carbohydrate intolerance observed in diabetic pregnancies. (author)

  19. Advances in male hormonal contraception.

    Science.gov (United States)

    Costantino, Antonietta; Gava, Giulia; Berra, Marta; Meriggiola Maria, Cristina

    2014-11-01

    Contraception is a basic human right for its role on health, quality of life and wellbeing of the woman and of the society as a whole. Since the introduction of female hormonal contraception the responsibility of family planning has always been with women. Currently there are only a few contraceptive methods available for men, but recently, men have become more interested in supporting their partners actively. Over the last few decades different trials have been performed providing important advances in the development of a safe and effective hormonal contraceptive for men. This paper summarizes some of the most recent trials. PMID:25673544

  20. Hormonal interaction in diabetic pregnancy

    Energy Technology Data Exchange (ETDEWEB)

    Hafiez, A.R.A.; Abdel-Hafez, M.A.; Osman, E.A. (Cairo Univ. (Egypt)); Ibrahim, M.S. (Al-Azhar Univ., Cairo (Egypt))

    1984-08-01

    Serum glucose, human placental lactogen (HPL), prolactin (PRL), estradiol (E/sub 2/), progesterone (P), cortisol and human growth hormone (HGH) were determined in nondiabetic (19 cases) and diabetic (19 cases) pregnant women during the 32nd and 36th week of gestation. Significant elevation of HPL, PRL, HGH and cortisol was found in the diabetic pregnant women during the 32nd week while E/sub 2/ and P were not significantly changed from the corresponding levels in the nondiabetic group. One can conclude that the changes in the hormonal pattern during gestation may induce carbohydrate intolerance observed in diabetic pregnancies.

  1. Advances in male hormonal contraception

    Directory of Open Access Journals (Sweden)

    Costantino Antonietta

    2014-01-01

    Full Text Available Contraception is a basic human right for its role on health, quality of life and wellbeing of the woman and of the society as a whole. Since the introduction of female hormonal contraception the responsibility of family planning has always been with women. Currently there are only a few contraceptive methods available for men, but recently, men have become more interested in supporting their partners actively. Over the last few decades different trials have been performed providing important advances in the development of a safe and effective hormonal contraceptive for men. This paper summarizes some of the most recent trials.

  2. Directory of Open Access Journals (Sweden)

    Laya Farzadi

    2012-06-01

    Full Text Available Introduction: Ovarian reserve depends on the size and quality of oocytes stored in ovary. Aging and some diseases reduce ovarian reserve, leading to decreased reproductive performance. Laparoscopic surgery is used to treat infertility in women with PCOS. The purpose of this study was measurement of ovarian reserve in patients with PCOS before and after laparoscopic surgery. Methods: In this study thirty infertile patients with PCOS that had not responded to initial treatment with drug were selected. We assessed the serum levels of anti-mullerian hormone, testosterone, luteinizing hormone (LH, and the number of antral follicles before laparoscopic surgery and 1 week, 3 and 6 months after the surgery. Results: The average amount of anti-mullerian hormone serum levels was 8.4±4.7 before laparoscopic surgery and 7.5±4.5, 7±4.5, and 7.7±4.4 ng/ml one week, 3 and 6 months after surgery, respectively. Serum levels of LH was 13.6±6.7 and 12.7±11.1 IU /ml (P=0.87 before surgery and 6 months after laparoscopy, respectively. Mean serum testosterone levels were 0.9±0.8, 0.8±0.9 ng/ml (P=0.86 before and after surgery. Annual reproductive rate was 26.7 percent in this population. Conclusion: Laparoscopic ovarian puncture didn’t change anti-mullerian hormone serum, testosterone and LH in women with PCOS and hence has no adverse effects on the ovarian reserve.

  3. Ultrasound in evaluating ovarian reserve

    Directory of Open Access Journals (Sweden)

    Eman Ahmaed Shawky Sabek

    2015-12-01

    Full Text Available The objective of this study was to compare the diagnostic accuracy of transvaginal ultrasound (TVS, as a less invasive technique instead of hormonal assay to evaluate the ovarian reserve. This study included fifty-five females with breast cancer and we compared the ovarian reserve for these patients by hormonal assay through measuring the serum AntiMullerian Hormone (AMH level and follicular stimulating hormone (FSH level before and after chemotherapy, and by transvaginal ultrasound through the ovarian volume (OV calculation and counting the Antral follicles (AFC before and after chemotherapy treatment. There was decline in the AntiMullerian Hormone level after chemotherapy by 27 ± 11.19% and decrease in the Antral follicle counts by 21 ± 13.43%. In conclusion there was strong relation between AMH level and AFC which makes the use of transvaginal ultrasound is a reliable alternative method to the hormonal assay to detect the ovarian reserve.

  4. The 'Love Hormone' May Quiet Tinnitus

    Science.gov (United States)

    ... medlineplus.gov/news/fullstory_161110.html The 'Love Hormone' May Quiet Tinnitus Small, preliminary study suggests oxytocin ... tinnitus -- may find some relief by spraying the hormone oxytocin in their nose, a small initial study ...

  5. Mortality and reduced growth hormone secretion

    DEFF Research Database (Denmark)

    Stochholm, Kirstine; Christiansen, Jens; Laursen, Torben;

    2007-01-01

    BACKGROUND: Data regarding the mortality rates of patients with growth hormone deficiency (GHD), whether or not treated with growth hormone (GH), are limited, but an increased mortality rate among hypopituitary patients compared with the general population has been documented. Cardiovascular...

  6. Investigation of suspected growth hormone deficiency

    OpenAIRE

    Milner, R. D. G.; Burns, E C

    1982-01-01

    This paper describes views of the Health Services Human Growth Hormone Committee on how a child suspected of growth hormone deficiency should be investigated in a district general hospital or in a regional growth centre.

  7. Hormone May Be Linked to Teenage Obesity

    Science.gov (United States)

    ... 159014.html Hormone May Be Linked to Teenage Obesity Researchers suspect low levels of spexin might play ... reduced levels of this hormone in adults with obesity. Overall, our findings suggest spexin may play a ...

  8. Hormone May Be Linked to Teenage Obesity

    Science.gov (United States)

    ... page: https://medlineplus.gov/news/fullstory_159014.html Hormone May Be Linked to Teenage Obesity Researchers suspect ... may have lower levels of a weight-regulating hormone than normal-weight teens, a new study says. " ...

  9. Sex hormone binding globulin phenotypes

    DEFF Research Database (Denmark)

    Cornelisse, M M; Bennett, Patrick; Christiansen, M;

    1994-01-01

    Human sex hormone binding globulin (SHBG) is encoded by a normal and a variant allele. The resulting SHBG phenotypes (the homozygous normal SHBG, the heterozygous SHBG and the homozygous variant SHBG phenotype) can be distinguished by their electrophoretic patterns. We developed a novel detection...

  10. Parathyroid hormone and bone healing

    DEFF Research Database (Denmark)

    Ellegaard, M; Jørgensen, N R; Schwarz, P

    2010-01-01

    , no pharmacological treatments are available. There is therefore an unmet need for medications that can stimulate bone healing. Parathyroid hormone (PTH) is the first bone anabolic drug approved for the treatment of osteoporosis, and intriguingly a number of animal studies suggest that PTH could be beneficial...

  11. Hormonal crosstalk in plant immunity

    NARCIS (Netherlands)

    van der Does, A.

    2012-01-01

    The plant hormones salicylic acid (SA), also known as plant aspirin, and jasmonic acid (JA) play major roles in the regulation of the plant immune system. In general, SA is important for defense against pathogens with a biotrophic lifestyle, whereas JA is essential for defense against insect herbivo

  12. Hormonal contraceptives and venous thrombosis

    NARCIS (Netherlands)

    Stegeman, Berendina Hendrika (Bernardine)

    2013-01-01

    Oral contraceptive use is associated with venous thrombosis. However, the mechanism behind this remains unclear. The aim of this thesis was to evaluate genetic variation in the first-pass metabolism of contraceptives, to identify the clinical implications of hormonal contraceptive use after a thromb

  13. Hormonal determinants of pubertal growth.

    NARCIS (Netherlands)

    Delamarre-van Waal, H.A.; Coeverden, S.C. van; Rotteveel, J.J.

    2001-01-01

    Pubertal growth results from increased sex steroid and growth hormone (GH) secretion. Estrogens appear to play an important role in the regulation of pubertal growth in both girls and boys. In girls, however, estrogens cannot be the only sex steroids responsible for pubertal growth, as exogenous est

  14. The Barrier Within: Endothelial Transport of Hormones

    OpenAIRE

    Kolka, Cathryn M.; Richard N Bergman

    2012-01-01

    Hormones are involved in a plethora of processes including development and growth, metabolism, mood, and immune responses. These essential functions are dependent on the ability of the hormone to access its target tissue. In the case of endocrine hormones that are transported through the blood, this often means that the endothelium must be crossed. Many studies have shown that the concentrations of hormones and nutrients in blood can be very different from those surrounding the cells on the t...

  15. Influence of ovarian hormones on urogenital infection

    OpenAIRE

    Sonnex, C

    1998-01-01

    Numerous studies have examined the influence of hormones on infectious diseases and there is now a wealth of data relating to the more specific effect of the sex hormones, oestrogen and progesterone, on urogenital infections. The interaction between these hormones and the immune system is complex and the variation of hormonal effect between species further complicates the true picture as related to humans. Although it is difficult therefore to draw general conclusions regarding predomin...

  16. Developing a model of plant hormone interactions

    OpenAIRE

    Wang, Yu Hua; Helen R Irving

    2011-01-01

    Plant growth and development is influenced by mutual interactions among plant hormones. The five classical plant hormones are auxins, cytokinins, gibberellins, abscisic acid and ethylene. They are small diffusible molecules that easily penetrate between cells. In addition, newer classes of plant hormones have been identified such as brassinosteroids, jasmonic acid, salicylic acid and various small proteins or peptides. These hormones also play important roles in the regulation of plant growth...

  17. Endocrine disruptors and thyroid hormone physiology

    OpenAIRE

    Jugan, Mary-Line; Levi, Yves; Blondeau, Jean-Paul

    2010-01-01

    Abstract Endocrine disruptors are man-made chemicals that can disrupt the synthesis, circulating levels, and peripheral action of hormones. The disruption of sex hormones was subject of intensive research, but thyroid hormone synthesis and signaling are now also recognized as important targets of endocrine disruptors. The neurological development of mammals is largely dependent on normal thyroid hormone homeostasis, and it is likely to be particularly sensitive to disruption of the...

  18. "Sex Hormones" in Secondary School Biology Textbooks

    Science.gov (United States)

    Nehm, Ross H.; Young, Rebecca

    2008-01-01

    This study explores the extent to which the term "sex hormone" is used in science textbooks, and whether the use of the term "sex hormone" is associated with pre-empirical concepts of sex dualism, in particular the misconceptions that these so-called "sex hormones" are sex specific and restricted to sex-related physiological functioning. We found…

  19. Peptide Hormones in the Gastrointestinal Tract

    DEFF Research Database (Denmark)

    Rehfeld, Jens F.

    2015-01-01

    Gastrointestinal hormones are peptides released from endocrine cells and neurons in the digestive tract. More than 30 hormone genes are currently known to be expressed in the gastrointestinal tract, which makes the gut the largest hormone-producing organ in the body. Modern biology makes it feasi...

  20. The hormonal regulation of life processes in insects (2.) The anti-juvenile hormones (1.)

    International Nuclear Information System (INIS)

    Those compounds that decrease either the level or activity of natural endogenous juvenile hormones in insects are called anti-juvenile hormones (AJH). The possible effects of anti-juvenile hormones are manifold: they may inhibit special enzymes or the bindings of juvenile hormones to receptors and transport proteins or may cause the destruction of corpora allata, the sources of juvenile hormones. The most obvious possibility to elicit an anti-juvenile hormone effect lies in the inhibition of enzymes participating in the biosynthesis of juvenile hormones

  1. Growth hormone state after completion of treatment with growth hormone.

    OpenAIRE

    Clayton, P E; Price, D. A.; Shalet, S M

    1987-01-01

    After completion of treatment with growth hormone (GH) 19 patients with isolated 'idiopathic' GH deficiency and 15 with post-irradiation GH deficiency underwent retesting of GH secretion with an insulin tolerance test or an arginine stimulation test, or both. Patients with post-irradiation GH deficiency comprised 13 patients with central nervous system tumours distant from the hypothalamo-pituitary axis and two with acute lymphoblastic leukaemia, who had received cranial or craniospinal irrad...

  2. Thyroid hormone receptors bind to defined regions of the growth hormone and placental lactogen genes.

    OpenAIRE

    Barlow, J W; Voz, M L; Eliard, P H; Mathy-Harter, M; De Nayer, Philippe; Economidis, I V; Belayew, A; Martial, J A; Rousseau, Guy

    1986-01-01

    The intracellular receptor for thyroid hormone is a protein found in chromatin. Since thyroid hormone stimulates transcription of the growth hormone gene through an unknown mechanism, the hypothesis that the thyroid hormone-receptor complex interacts with defined regions of this gene has been investigated in a cell-free system. Nuclear extracts from human lymphoblastoid IM-9 cells containing thyroid hormone receptors were incubated with L-3,5,3'-tri[125I]iodothyronine and calf thymus DNA-cell...

  3. The menopause and hormone replacement therapy: views of women in general practice receiving hormone replacement therapy.

    OpenAIRE

    Roberts, P J

    1991-01-01

    Women's views on the menopause and hormone replacement therapy were explored using a questionnaire given to women attending one general practice who were having hormone replacement therapy under the supervision of their doctor. Sixty four women (67%) responded. Although only 5% of women had requested hormone replacement therapy from their general practitioner the majority of women indicated that they had been helped by hormone replacement therapy. Eight per cent of women were using hormone re...

  4. Reproductive Hormones and Mood Disorders

    Directory of Open Access Journals (Sweden)

    Sermin Kesebir

    2010-12-01

    Full Text Available During the menstrual cycle, pregnancy and breast-feeding periods, as well as in menopausal and post-menopausal periods, the physiological and psychological processes that change according to the hormonal fluctuations influence every women similarly and each one differently. These physiological processes are controlled by neuroendocrine sequences, of which the hypothalamo-pituitary-adrenal axis and the hypothalamo-pituitary-gonadal axis are the most important ones. The hypothalamo-pituitary-gonadal axis affects mood, anxiety, cognition and pain. The interaction of these hormones with mood and behavior is bidirectional. The differences in phenomenology and epidemiology of mood disorders with regards to gender can be explained with the effects of hormones. All of the periods mentioned above are related with mood disorders at terms of risk factors, disease symptoms, progress of disease and response to treatment. Epidemiologic data supports the relationship between the mood disorders and reproductive processes. The prevalence of major depression increases in women with the menarche and ceases in post- menopausal period. Similarly, the initial symptoms of bipolar disorder begins around the menarche period in 50% of the cases. Despite proper treatment, some female patients with major depression experience recurrence during the premenstrual period of their menstrual cycles. The conformity and change in a woman’s brain during pregnancy is controlled dominantly by the neuroendocrine systems, while it is controlled by the external stimuli actively related to the baby during nursing period. The changes that occur are closely related to postpartum mood disorders. Again, all the changes and suspension of medication during this procedure are risk factors for early depressive and dysphoric situations. Variables of a wide range, from follicle stimulating hormone, melatonin, and sleep to body mass index interact with mood disorders in menopausal and post

  5. Hormonal treatment of acne vulgaris: an update.

    Science.gov (United States)

    Elsaie, Mohamed L

    2016-01-01

    Acne vulgaris is a common skin condition associated with multiple factors. Although mostly presenting alone, it can likewise present with features of hyperandrogenism and hormonal discrepancies. Of note, hormonal therapies are indicated in severe, resistant-to-treatment cases and in those with monthly flare-ups and when standard therapeutic options are inappropriate. This article serves as an update to hormonal pathogenesis of acne, discusses the basics of endocrinal evaluation for patients with suspected hormonal acne, and provides an overview of the current hormonal treatment options in women. PMID:27621661

  6. Hormonal treatment of acne vulgaris: an update

    Science.gov (United States)

    Elsaie, Mohamed L

    2016-01-01

    Acne vulgaris is a common skin condition associated with multiple factors. Although mostly presenting alone, it can likewise present with features of hyperandrogenism and hormonal discrepancies. Of note, hormonal therapies are indicated in severe, resistant-to-treatment cases and in those with monthly flare-ups and when standard therapeutic options are inappropriate. This article serves as an update to hormonal pathogenesis of acne, discusses the basics of endocrinal evaluation for patients with suspected hormonal acne, and provides an overview of the current hormonal treatment options in women. PMID:27621661

  7. Hormonal contraception, thrombosis and age

    DEFF Research Database (Denmark)

    Lidegaard, Øjvind

    2014-01-01

    INTRODUCTION: This paper reviews the risk of thrombosis with use of different types of hormonal contraception in women of different ages. AREAS COVERED: Combined hormonal contraceptives with desogestrel, gestodene, drospirenone or cyproterone acetate (high-risk products) confer a sixfold increased......: First choice in women below 35 years should be a combined low-risk pill, that is, with a second-generation progestin, with the lowest compliable dose of estrogen. Young women with risk factors of thrombosis such as age above 35 years, genetic predispositions, adiposity, polycystic ovary syndrome......, diabetes, smoking, hypertension or migraine with aura should not use high-risk products, but should primarily consider progestin-only products, and be careful to use low-risk combined products....

  8. Radioactive labelling of peptidic hormones

    International Nuclear Information System (INIS)

    The labelling of peptidic hormones requires stability, specificity and sensitivity of the label. Introduction of a radioactive atome is one way to satisfy these criteria. Several processes have been described to prepare radioactive TRF: synthesis of the peptide with labelled aminoacids or introduction of the label into the hormone. In that approach, tritium can be substituted in the imidazole ring, via precursors activating the proper carbon. Monoiodo TRF leads essentially to tritium labelling of the 5 positions whereas monoazo TRF allows the preparation of 3H TRF labelled in the 2 positions. Di-substituted TRF leads to labelling into the 2 and 5 carbons. Labelled analogs of TRF can be prepared with labelled iodine; further developments of peptide labelling, will be presented. In particular, the homolytic scission of the C-iodine, bond by photochemical activation. The nascent carbon radical can be stabilized by a tritiated scavenger. This approach eliminates the use of heavy metal catalysts

  9. Growth hormone and its disorders

    OpenAIRE

    Ayuk, J.; Sheppard, M C

    2006-01-01

    Growth hormone (GH) is synthesised and secreted by the somatotroph cells of the anterior lobe of the pituitary gland. Its actions involve multiple organs and systems, affecting postnatal longitudinal growth as well as protein, lipid, and carbohydrate metabolism. GH hypersecretion results in gigantism or acromegaly, a condition associated with significant morbidity and mortality, while GH deficiency results in growth retardation in children and the GH deficiency syndrome in adults. This articl...

  10. Hormones, genes, and behavior

    OpenAIRE

    Pfaff, Donald W.

    1997-01-01

    With assays of hormone-sensitive behaviors, it is possible to demonstrate both direct and indirect actions of genes on mammalian social behaviors. Direct effects of estrogen receptor gene expression and progesterone receptor gene expression figure prominently in well analyzed neuroendocrine mechanisms for sex behavior, operating through a neural circuit that has been delineated. Indirect effects, notably the consequences of sexual differentiation, display complex d...

  11. Parathyroid Hormone Levels and Cognition

    Science.gov (United States)

    Burnett, J.; Smith, S.M.; Aung, K.; Dyer, C.

    2009-01-01

    Hyperparathyroidism is a well-recognized cause of impaired cognition due to hypercalcemia. However, recent studies have suggested that perhaps parathyroid hormone itself plays a role in cognition, especially executive dysfunction. The purpose of this study was to explore the relationship of parathyroid hormone levels in a study cohort of elders with impaied cognition. Methods: Sixty community-living adults, 65 years of age and older, reported to Adult Protective Services for self-neglect and 55 controls matched (on age, ethnicity, gender and socio-economic status) consented and participated in this study. The research team conducted in-home comprehensive geriatric assessments which included the Mini-mental state exam (MMSE), the 15-item geriatric depression scale (GDS) , the Wolf-Klein clock test and a comprehensive nutritional panel, which included parathyroid hormone and ionized calcium. Students t tests and linear regression analyses were performed to assess for bivariate associations. Results: Self-neglecters (M = 73.73, sd=48.4) had significantly higher PTH levels compared to controls (M =47.59, sd=28.7; t=3.59, df=98.94, plevels. Overall, PTH was correlated with the MMSE (r=-.323, p=.001). Individual regression analyses revealed a statistically significant correlation between PTH and MMSE in the self-neglect group (r=-.298, p=.024) and this remained significant after controlling for ionized calcium levels in the regression. No significant associations were revealed in the control group or among any of the other cognitive measures. Conclusion: Parathyroid hormone may be associated with cognitive performance.

  12. Reproductive Hormones and Mood Disorders

    OpenAIRE

    Sermin Kesebir; Arzu Etlik Aksoy

    2010-01-01

    During the menstrual cycle, pregnancy and breast-feeding periods, as well as in menopausal and post-menopausal periods, the physiological and psychological processes that change according to the hormonal fluctuations influence every women similarly and each one differently. These physiological processes are controlled by neuroendocrine sequences, of which the hypothalamo-pituitary-adrenal axis and the hypothalamo-pituitary-gonadal axis are the most important ones. The hypothalamo-pituitary-go...

  13. Growth hormone, inflammation and aging

    Directory of Open Access Journals (Sweden)

    Michal M. Masternak

    2012-04-01

    Full Text Available Mutant animals characterized by extended longevity provide valuable tools to study the mechanisms of aging. Growth hormone and insulin-like growth factor-1 (IGF-1 constitute one of the well-established pathways involved in the regulation of aging and lifespan. Ames and Snell dwarf mice characterized by GH deficiency as well as growth hormone receptor/growth hormone binding protein knockout (GHRKO mice characterized by GH resistance live significantly longer than genetically normal animals. During normal aging of rodents and humans there is increased insulin resistance, disruption of metabolic activities and decline of the function of the immune system. All of these age related processes promote inflammatory activity, causing long term tissue damage and systemic chronic inflammation. However, studies of long living mutants and calorie restricted animals show decreased pro-inflammatory activity with increased levels of anti-inflammatory adipokines such as adiponectin. At the same time, these animals have improved insulin signaling and carbohydrate homeostasis that relate to alterations in the secretory profile of adipose tissue including increased production and release of anti-inflammatory adipokines. This suggests that reduced inflammation promoting healthy metabolism may represent one of the major mechanisms of extended longevity in long-lived mutant mice and likely also in the human.

  14. Thyroid hormone and seasonal rhythmicity

    Directory of Open Access Journals (Sweden)

    Hugues eDardente

    2014-02-01

    Full Text Available Living organisms show seasonality in a wide array of functions such as reproduction, fattening, hibernation and migration. At temperate latitudes, changes in photoperiod maintain the alignment of annual rhythms with predictable changes in the environment. The appropriate physiological response to changing photoperiod in mammals requires retinal detection of light and pineal secretion of melatonin, but extraretinal detection of light occurs in birds. A common mechanism across all vertebrates is that these photoperiod-regulated systems alter hypothalamic thyroid hormone conversion. Here we review the evidence that a circadian clock within the pars tuberalis of the adenohypophysis links photoperiod decoding to local changes of thyroid hormone signalling within the medio-basal hypothalamus through a conserved thyrotropin/deiodinase axis. We also focus on recent findings which indicate that, beyond the photoperiodic control of its conversion, thyroid hormone might also be involved in longer term timing processes of seasonal programs. Finally, we examine the potential implication of kisspeptin and RFRP3, two RF-amide peptides expressed within the medio-basal hypothalamus, in seasonal rhythmicity.

  15. Scrotal approach for the correction of an abdominoscrotal hydrocele: Medium term follow-up

    Directory of Open Access Journals (Sweden)

    Jaime Pérez,

    2015-06-01

    Full Text Available Describe the scrotal surgical approach for abdominoscrotal hydrocele (ASH with preservation of testicular function with midterm follow-up. Two patients diagnosed with ASH and contralateral testicular abnormalities. Both had scrotal correction for ASH, one had contralateral testicular neonatal torsion and the other contralateral communicating hydrocele. Four-year follow-up no complications or recurrence, and normal Anti-Mullerian hormone level. This pathology is a clinical diagnosis and it is treated with surgery via a scrotal approach, preventing sequels, contralateral complications and preservation of testicular function.

  16. Effect of growth hormone replacement therapy on pituitary hormone secretion and hormone replacement therapies in GHD adults

    DEFF Research Database (Denmark)

    Hubina, Erika; Mersebach, Henriette; Rasmussen, Ase Krogh;

    2004-01-01

    We tested the impact of commencement of GH replacement therapy in GH-deficient (GHD) adults on the circulating levels of other anterior pituitary and peripheral hormones and the need for re-evaluation of other hormone replacement therapies, especially the need for dose changes.......We tested the impact of commencement of GH replacement therapy in GH-deficient (GHD) adults on the circulating levels of other anterior pituitary and peripheral hormones and the need for re-evaluation of other hormone replacement therapies, especially the need for dose changes....

  17. Anabolic hormone profiles in elite military men.

    Science.gov (United States)

    Taylor, Marcus K; Kviatkovsky, Shiloah A; Hernández, Lisa M; Sargent, Paul; Segal, Sabrina; Granger, Douglas A

    2016-06-01

    We recently characterized the awakening responses and daily profiles of the catabolic stress hormone cortisol in elite military men. Anabolic hormones follow a similar daily pattern and may counteract the catabolic effects of cortisol. This companion report is the first to characterize daily profiles of anabolic hormones dehydroepiandrosterone (DHEA) and testosterone in this population. Overall, the men in this study displayed anabolic hormone profiles comparable to that of healthy, athletic populations. Consistent with the cortisol findings in our prior report, summary parameters of magnitude (hormone output) within the first hour after awakening displayed superior stability versus summary parameters of pattern for both DHEA (r range: 0.77-0.82) and testosterone (r range: 0.62-0.69). Summary parameters of evening function were stable for the two hormones (both panabolic balance and resultant effects upon health and human performance in this highly resilient yet chronically stressed population. PMID:27083310

  18. Anabolic hormone profiles in elite military men.

    Science.gov (United States)

    Taylor, Marcus K; Kviatkovsky, Shiloah A; Hernández, Lisa M; Sargent, Paul; Segal, Sabrina; Granger, Douglas A

    2016-06-01

    We recently characterized the awakening responses and daily profiles of the catabolic stress hormone cortisol in elite military men. Anabolic hormones follow a similar daily pattern and may counteract the catabolic effects of cortisol. This companion report is the first to characterize daily profiles of anabolic hormones dehydroepiandrosterone (DHEA) and testosterone in this population. Overall, the men in this study displayed anabolic hormone profiles comparable to that of healthy, athletic populations. Consistent with the cortisol findings in our prior report, summary parameters of magnitude (hormone output) within the first hour after awakening displayed superior stability versus summary parameters of pattern for both DHEA (r range: 0.77-0.82) and testosterone (r range: 0.62-0.69). Summary parameters of evening function were stable for the two hormones (both panabolic balance and resultant effects upon health and human performance in this highly resilient yet chronically stressed population.

  19. Ectopic acromegaly due to growth hormone releasing hormone.

    Science.gov (United States)

    Ghazi, Ali A; Amirbaigloo, Alireza; Dezfooli, Azizollah Abbasi; Saadat, Navid; Ghazi, Siavash; Pourafkari, Marina; Tirgari, Farrokh; Dhall, Dheepti; Bannykh, Serguei; Melmed, Shlomo; Cooper, Odelia

    2013-04-01

    Acromegaly secondary to extra-pituitary tumors secreting growth hormone releasing hormone (GHRH) is rarely encountered. We review the literature on ectopic acromegaly and present the index report of ectopic acromegaly secondary to GHRH secretion from a mediastinal paraganglioma. Clinical and pathological manifestations and therapeutic management of 99 patients with ectopic acromegaly are reviewed. Acromegaly secondary to ectopic GHRH secretion is usually caused by a neuroendocrine tumor in the lung and pancreas. We report an additional cause of ectopic acromegaly from a mediastinal paraganglioma. Diagnostic criteria of ectopic GHRH syndrome include biochemical and pathologic tumoral confirmation of GHRH secretion and expression. Management of ectopic acromegaly consists of surgical resection of the primary tumor and biochemical normalization, with possible adjuvant use of somatostatin analogs. The review demonstrates that there are several tumor types, including paragangliomas which may secrete GHRH, leading to acromegaly. Clinical and laboratory manifestations of the syndrome and challenges in diagnosis and management of these rarely encountered patients require early diagnosis and appropriate treatment to prevent long-term morbidity and mortality with ectopic acromegaly. PMID:22983831

  20. Aluminum, parathyroid hormone, and osteomalacia

    Energy Technology Data Exchange (ETDEWEB)

    Burnatowska-Hledin, M.A.; Kaiser, L.; Mayor, G.H.

    1983-01-01

    Aluminum exposure in man is unavoidable. The occurrence of dialysis dementia, vitamin D-resistant osteomalacia, and hypochromic microcytic anemia in dialysis patients underscores the potential for aluminum toxicity. Although exposure via dialysate and hyperalimentation leads to significant tissue aluminum accumulation, the ubiquitous occurrence of aluminum and the severe pathology associated with large aluminum burdens suggest that smaller exposures via the gastrointestinal tract and lungs could represent an important, though largely unrecognized, public health problem. It is clear that some aluminum absorption occurs with the ingestion of small amounts of aluminum in the diet and medicines, and even greater aluminum absorption is seen in individuals consuming large amounts of aluminum present in antacids. Aluminum absorption is enhanced in the presence of elevated circulating parathyroid hormone. In addition, elevated PTH leads to the preferential deposition of aluminum in brain and bone. Consequently, PTH is likely to be involved in the pathogenesis of toxicities in those organs. PTH excess also seems to lead to the deposition of aluminum in the parathyroid gland. The in vitro demonstration that aluminum inhibits parathyroid hormone release is consistent with the findings of a euparathyroid state in dialysis patients with aluminum related vitamin D-resistant osteomalacia. Nevertheless, it seems likely that hyperparathyroidism is at least initially involved in the pathogenesis of aluminum neurotoxicity and osteomalacia; the increases in tissue aluminum stores are followed by suppression of parathyroid hormone release, which is required for the evolution of osteomalacia. Impaired renal function is not a prerequisite for increased tissue aluminum burdens, nor for aluminum-related organ toxicity. Consequently, it is likely that these diseases will be observed in populations other than those with chronic renal disease.

  1. Human growth hormone (HGH), ch. 6

    International Nuclear Information System (INIS)

    A radioimmunoassay method for the human growth hormone (HGH) is described. The requirements are discussed in detail and a scheme for the preparation of incubation mixtures is given. HGH is labelled with 125I by the chloramine T method and purified by gel filtration or electrophoresis. Separation of bound and free-labelled hormones is performed by absorption of the free hormone, using talc or charcoal

  2. Hormones and aggression in childhood and adolescence

    OpenAIRE

    Ramirez, J. Martin

    2003-01-01

    This review is a survey on recent psychobiosocial studies on association between hormones and aggression/violence in children and adolescents, with a special focus on puberty, given the rapid changes in both hormones and behavior occurring during that developmental period. Since it cannot be assumed that all readers have much background knowledge, it inevitably begins with some comments about the concept and multifaceted nature of aggression, as well as with a brief reminding about hormone ca...

  3. Plant Hormones: Metabolism, Signaling and Crosstalk

    Institute of Scientific and Technical Information of China (English)

    Li-Jia Qu; Yunde Zhao

    2011-01-01

    @@ Plants synthesize various hormones in response to environmental cues and developmental signals to ensure their proper growth and development.Elucidation of the molecular mechanisms by which plant hormones control growth and development contributes to our understanding of fundamental plant biology and provides tools to improve crops.Because of their critical roles in plant growth and development, plant hormones have been studied extensively since the early days of plant biology.

  4. Monitoring Plant Hormones During Stress Responses

    OpenAIRE

    Engelberth, Marie J.; Engelberth, Jurgen

    2009-01-01

    Plant hormones and related signaling compounds play an important role in the regulation of plant responses to various environmental stimuli and stresses. Among the most severe stresses are insect herbivory, pathogen infection, and drought stress. For each of these stresses a specific set of hormones and/or combinations thereof are known to fine-tune the responses, thereby ensuring the plant's survival. The major hormones involved in the regulation of these responses are jasmonic acid (JA), sa...

  5. Gastric emptying, glucose metabolism and gut hormones

    DEFF Research Database (Denmark)

    Vermeulen, Mechteld A R; Richir, Milan C; Garretsen, Martijn K;

    2011-01-01

    To study the gastric-emptying rate and gut hormonal response of two carbohydrate-rich beverages. A specifically designed carbohydrate-rich beverage is currently used to support the surgical patient metabolically. Fruit-based beverages may also promote recovery, due to natural antioxidant...... and carbohydrate content. However, gastric emptying of fluids is influenced by its nutrient composition; hence, safety of preoperative carbohydrate loading should be confirmed. Because gut hormones link carbohydrate metabolism and gastric emptying, hormonal responses were studied....

  6. Pharmacologic development of male hormonal contraceptive agents.

    Science.gov (United States)

    Roth, M Y; Amory, J K

    2011-01-01

    The world population continues to increase dramatically despite the existence of contraceptive technology. The use of male hormonal contraception may help in preventing un intended pregnancies and managing future population growth. Male hormonal contraception relies on the administration of exogenous hormones to suppress spermatogenesis. Clinical trials have tested several regimens using testosterone, alone or in combination with a progestin. These regimens were shown to be >90% effective in preventing conception and were not associated with serious adverse events.

  7. Growth hormone and its disorders.

    Science.gov (United States)

    Ayuk, J; Sheppard, M C

    2006-01-01

    Growth hormone (GH) is synthesised and secreted by the somatotroph cells of the anterior lobe of the pituitary gland. Its actions involve multiple organs and systems, affecting postnatal longitudinal growth as well as protein, lipid, and carbohydrate metabolism. GH hypersecretion results in gigantism or acromegaly, a condition associated with significant morbidity and mortality, while GH deficiency results in growth retardation in children and the GH deficiency syndrome in adults. This article, aimed at non-paediatric physicians, examines the clinical features, diagnosis, and current concepts in the management of these conditions. PMID:16397076

  8. [Laboratory diagnosis of growth hormone].

    Science.gov (United States)

    Macchia, V; Mariano, A

    1993-09-01

    The role of Clinical Pathology Laboratory in normal and altered growth hormone secretion is discussed. In particular, it is reported that the normal GH secretion can be studied by serum and urine GH determinations whereas the diagnosis of GH deficiency rests upon the demonstration of an inadequate rise serum GH after provocative stimuli and serum measurement of somatomedins (IGF-I) by radioimmunoassay method. As it concerns increase GH secretion the diagnosis is clinically made (acromegaly and gigantism) and the laboratory has only the role to confirm it by the assessment of basal and stimulated GH secretion. PMID:7910655

  9. Hormonal contraceptives and venous thrombosis

    OpenAIRE

    Stegeman, Berendina Hendrika (Bernardine)

    2013-01-01

    Oral contraceptive use is associated with venous thrombosis. However, the mechanism behind this remains unclear. The aim of this thesis was to evaluate genetic variation in the first-pass metabolism of contraceptives, to identify the clinical implications of hormonal contraceptive use after a thrombotic event and to provide an overview of the risk of venous thrombosis per combined oral contraceptive. We found that the UGT2B7 gene in the first-pass metabolism may at least in part explain the r...

  10. Leptin and Hormones: Energy Homeostasis.

    Science.gov (United States)

    Triantafyllou, Georgios A; Paschou, Stavroula A; Mantzoros, Christos S

    2016-09-01

    Leptin, a 167 amino acid adipokine, plays a major role in human energy homeostasis. Its actions are mediated through binding to leptin receptor and activating JAK-STAT3 signal transduction pathway. It is expressed mainly in adipocytes, and its circulating levels reflect the body's energy stores in adipose tissue. Recombinant methionyl human leptin has been FDA approved for patients with generalized non-HIV lipodystrophy and for compassionate use in subjects with congenital leptin deficiency. The purpose of this review is to outline the role of leptin in energy homeostasis, as well as its interaction with other hormones. PMID:27519135

  11. Antimüllerian hormone in gonadotropin releasing-hormone antagonist cycles

    DEFF Research Database (Denmark)

    Arce, Joan-Carles; La Marca, Antonio; Mirner Klein, Bjarke;

    2013-01-01

    To assess the relationships between serum antimüllerian hormone (AMH) and ovarian response and treatment outcomes in good-prognosis patients undergoing controlled ovarian stimulation using a gonadotropin-releasing hormone (GnRH) antagonist protocol.......To assess the relationships between serum antimüllerian hormone (AMH) and ovarian response and treatment outcomes in good-prognosis patients undergoing controlled ovarian stimulation using a gonadotropin-releasing hormone (GnRH) antagonist protocol....

  12. Hormone-Sensitive Lipase Knockouts

    Directory of Open Access Journals (Sweden)

    Shen Wen-Jun

    2006-02-01

    Full Text Available Abstract All treatments for obesity, including dietary restriction of carbohydrates, have a goal of reducing the storage of fat in adipocytes. The chief enzyme responsible for the mobilization of FFA from adipose tissue, i.e., lipolysis, is thought to be hormone-sensitive lipase (HSL. Studies of HSL knockouts have provided important insights into the functional significance of HSL and into adipose metabolism in general. Studies have provided evidence that HSL, though possessing triacylglycerol lipase activity, appears to be the rate-limiting enzyme for cholesteryl ester and diacylglycerol hydrolysis in adipose tissue and is essential for complete hormone stimulated lipolysis, but other triacylglycerol lipases are important in mediating triacylglycerol hydrolysis in lipolysis. HSL knockouts are resistant to both high fat diet-induced and genetic obesity, displaying reduced quantities of white with increased amounts of brown adipose tissue, increased numbers of adipose macrophages, and have multiple alterations in the expression of genes involved in adipose differentiation, including transcription factors, markers of adipocyte differentiation, and enzymes of fatty acid and triglyceride synthesis. With disruption of lipolysis by removal of HSL, there is a drastic reduction in lipogenesis and alteration in adipose metabolism.

  13. Postmenopausal hormone therapy and cognition

    Science.gov (United States)

    McCarrey, Anna C.; Resnick, Susan M.

    2015-01-01

    Prior to the publication of findings from the Women’s Health Initiative (WHI) in 2002, estrogen-containing hormone therapy (HT) was used to prevent age-related disease, especially cardiovascular disease, and to treat menopausal symptoms such as hot flushes and sleep disruptions. Some observational studies of HT in midlife and aging women suggested that HT might also benefit cognitive function, but randomized clinical trials have produced mixed findings in terms of health and cognitive outcomes. This review focuses on hormone effects on cognition and risk for dementia in naturally menopausal women as well as surgically induced menopause, and highlights findings from the large-scale WHI Memory Study (WHIMS) which, contrary to expectation, showed increased dementia risk and poorer cognitive outcomes in older postmenopausal women randomized to HT versus placebo. We consider the ‘critical window hypothesis’, which suggests that a window of opportunity may exist shortly after menopause during which estrogen treatments are most effective. In addition, we highlight emerging evidence that potential adverse effects of HT on cognition are most pronounced in women who have other health risks, such as cerebrovascular disease or diabetes. Lastly, we point towards implications for future research and clinical treatments. PMID:25935728

  14. Pituitary mammosomatotroph adenomas develop in old mice transgenic for growth hormone-releasing hormone

    DEFF Research Database (Denmark)

    Asa, S L; Kovacs, K; Stefaneanu, L;

    1990-01-01

    It has been shown that mice transgenic for human growth hormone-releasing hormone (GRH) develop hyperplasia of pituitary somatotrophs and mammosomatotrophs, cells capable of producing both growth hormone and prolactin, by 8 months of age. We now report for the first time that old GRH-transgenic m...

  15. Thyroid Hormone Receptor beta Mediates Acute Illness-Induced Alterations in Central Thyroid Hormone Metabolism

    NARCIS (Netherlands)

    A. Boelen; J. Kwakkel; O. Chassande; E. Fliers

    2009-01-01

    Acute illness in mice profoundly affects thyroid hormone metabolism in the hypothalamus and pituitary gland. It remains unknown whether the thyroid hormone receptor (TR)-beta is involved in these changes. In the present study, we investigated central thyroid hormone metabolism during lipopolysacchar

  16. Action of luteinizing hormone-releasing hormone in rat ovarian cells: Hormone production and signal transduction

    Energy Technology Data Exchange (ETDEWEB)

    Wang, Jian.

    1989-01-01

    The present study was conducted to investigate the hypothesis that the breakdown of membrane phosphoinositides may participate in the actions of luteinizing hormone-releasing hormone (LHRH) on hormone production in rat granulosa cells. In cells prelabeled with ({sup 3}H)inositol or ({sup 3}H)arachidonic acid (AA), treatment with LHRH increased the formation of radiolabeled inositol 1,4,5-trisphosphate (IP{sub 3}) and diacylglycerol (DG), and the release of radiolabeled AA. Since IP{sub 3} induces intracellular Ca{sup 2+} mobilization, changes in the cytosolic free calcium ion concentrations ((Ca{sup 2+})i) induced by LHRH were studied in individual cells using fura-2 microspectrofluorimetry. Alterations in (Ca{sup 2+})i induced by LHRH were rapid and transient, and could be completely blocked by a LHRH antagonist. Sustained perifusion of LHRH resulted in a desensitization of the (Ca{sup 2+})i response to LHRH. LHRH treatment accelerated (Ca{sup 2+})i depletion in the cells perifused with Ca{sup 2+} free medium, indicating the involvement of intracellular Ca{sup 2+} pool(s) in (Ca{sup 2+})i changes. The actions of LHRH on the regulation of progesterone (P{sub 4}) and prostaglandin E{sub 2} (PGE{sub 2}) production were also examined. LHRH increased basal P{sub 4} production and attenuated FSH induced P{sub 4} production. Both basal and FSH stimulated PGE{sub 2} formation were increased by LHRH. Since LHRH also increased the formation of DG that stimulates the activity of protein kinase C, an activator of protein kinase C (12-0-tetradecanolyphorbol-13-acetate: TPA) was used with the Ca{sup 2+} ionophore A23187 and melittin (an activator of phospholipase A{sub 2}) to examine the roles of protein kinase C, Ca{sup 2+} and free AA, respectively, in LHRH action.

  17. Growth Hormone Response after Administration of L-dopa, Clonidine, and Growth Hormone Releasing Hormone in Children with Down Syndrome.

    Science.gov (United States)

    Pueschel, Seigfried M.

    1993-01-01

    This study of eight growth-retarded children with Down's syndrome (aged 1 to 6.5 years) found that administration of growth hormone was more effective than either L-dopa or clonidine. Results suggest that children with Down's syndrome have both anatomical and biochemical hypothalamic derangements resulting in decreased growth hormone secretion and…

  18. 血清抗苗勒管激素水平在多囊卵巢综合征的临床意义%Clinical significance of serum anti-Müllerian hormone level in polycystic ovary syndrome

    Institute of Scientific and Technical Information of China (English)

    侯延庆; 叶元

    2013-01-01

    目的 探讨血清抗苗勒管激素(AMH)水平检测在多囊卵巢综合征(PCOS)中的临床意义.方法 选择PCOS患者80例(PCOS组),其中具有以下情况之一者纳入PCOS组1(40例):月经正常、高雄激素血症和多囊卵巢(PCO);月经稀少或闭经、雄激素正常和PCO;月经稀少或闭经、高雄激素血症和无PCO.月经稀少或闭经、高雄激素血症和PCO患者纳入PCOS组2.40例年龄和体质量指数(BMI)相匹配的健康妇女纳入对照组.检测各研究组血清AMH水平及各项相关指标.结果 PCOS组血清AMH(55.21±9.03) pmol/L高于对照组(12.58±4.82)pmol/L(P<0.05),PCOS组2血清AMH(61.73±7.62)pmol/L高于PCOS组1(32.53±3.05)pmol/L(P<0.05);对照组及PCOS组血清AMH均与小卵泡数(FN)呈正相关(相关系数分别为0.856、0.703,P<0.05);对照组血清AMH与年龄呈负相关(r=-0.431,P<0.05).结论 AMH分泌异常可能与PCOS发生发展有关,血清AMH水平是描述PCO的可靠指标,也是反映健康妇女卵巢功能衰退的敏感标志物,而在PCOS患者中可能无此作用.%Objective To explore the clinical significance of serum anti-Mullerian hormone(AMH) level in polycystic ovary syn-drome(PCOS).Methods Serum AMH concentrations were tested in 80 women with polycystic ovary syndrome(PCOS group) and 40 controls.Women with PCOS were divided into two groups.PCOS groupl included women with one of three conditions, including condition 1 of with normal menses,hyperandrogenemia(HA) and polycystic ovarian morphology ( PCO ), condition 2 of with oligo-or amenorrhea(ANOV) , normal androgen and PCO, condition 3 of with oligo- or ANOV, HA and PCO.PCOS group2 included women with oligo- or ANOV, HA and PCO.Other serum indexes related to PCOS were also tested.Results Serum AMH level of PCOS group was (55.21±9.03)pmol/L,higher than (12.58±4.82)pmol/L of control group(P<0.05).Serum AMH level of P-COS group2 was (61.73 ± 7.62)pmol/L,higher than (32.53 ± 3.05)pmol/L in PCOS groupl(P<0

  19. Glucoregulatory function of thyroid hormones: role of pancreatic hormones

    International Nuclear Information System (INIS)

    Glucose metabolism was investigated in humans before and 14 days after 300 micrograms L-thyroxine (T4)/day using a sequential clamp protocol during short-term somatostatin infusion (500 micrograms/h, 0-6 h) at euglycemia (0-2.5 h), at 165 mg/dl (2.5-6 h), and during insulin infusion (1.0 mU.kg-1.min-1, 4.5-6 h). T4 treatment increased plasma T4 (+96%) and 3,5,3'-triiodothyronine (T3, +50%), energy expenditure (+8%), glucose turnover (+32%), and glucose oxidation (Glucox +87%) but decreased thyroid-stimulating hormone (-96%) and nonoxidative glucose metabolism (Glucnonox, -30%) at unchanged lipid oxidation (Lipox). During somatostatin and euglycemia glucose production (Ra, -67%) and disposal (Rd, -28%) both decreased in euthyroid subjects but remained at -22% and -5%, respectively, after T4 treatment. Glucox (control, -20%; +T4, -25%) fell and Lipox increased (control, +42%; +T4, +45%) in both groups, whereas Glucnonox decreased before (-36%) but increased after T4 (+57%). During somatostatin infusion and hyperglycemia Rd (control, +144%; +T4, +84%) and Glucnonox (control, +326%; +T4, +233%) increased, whereas Glucox and Lipox remained unchanged. Insulin further increased Rd (+76%), Glucox (+155%), and Glucnonox (+50%) but decreased Ra (-43%) and Lipox (-43%). All these effects were enhanced by T4 (Rd, +38%; Glucox, +45%; Glucnonox, +35%; Ra, +40%; Lipox, +11%). Our data provide evidence that, in humans, T3 stimulates Ra and Rd, which is in part independent of pancreatic hormones

  20. Sweat secretion rates in growth hormone disorders

    DEFF Research Database (Denmark)

    Sneppen, S B; Main, K M; Juul, A;

    2000-01-01

    While increased sweating is a prominent symptom in patients with active acromegaly, reduced sweating is gaining status as part of the growth hormone deficiency (GHD) syndrome.......While increased sweating is a prominent symptom in patients with active acromegaly, reduced sweating is gaining status as part of the growth hormone deficiency (GHD) syndrome....

  1. Menstrual cycle hormones, food intake, and cravings

    Science.gov (United States)

    Objective: Food craving and intake are affected by steroid hormones during the menstrual cycle, especially in the luteal phase, when craving for certain foods has been reported to increase. However, satiety hormones such as leptin have also been shown to affect taste sensitivity, and therefore food ...

  2. Hormones and absence epilepsy in genetic models

    NARCIS (Netherlands)

    Tolmacheva, E.A.; Luijtelaar, E.L.J.M. van

    2010-01-01

    Steroid hormones are known to have a tremendous impact on seizures and might play a prominent role in epileptogenesis. However, little is known about the role of steroid hormones in absence epilepsy. Here we review recently combined electrophysiological, pharmacological and behavioural studies in a

  3. Measuring Steroid Hormones in Avian Eggs

    NARCIS (Netherlands)

    Engelhardt, Nikolaus von; Groothuis, Ton G.G.

    2005-01-01

    Avian eggs contain substantial levels of various hormones of maternal origin and have recently received a lot of interest, mainly from behavioral ecologists. These studies strongly depend on the measurement of egg hormone levels, but the method of measuring these levels has received little attention

  4. Sequential growth hormone deficiency and acromegaly.

    OpenAIRE

    Heffernan, A.

    1988-01-01

    This is the case of a patient with a pituitary tumour presenting initially with growth hormone deficiency and requiring treatment with human growth hormone. Eight years later he represented with acromegaly. This sequence of events has not to my knowledge been reported previously.

  5. Clinical Trials in Male Hormonal Contraception

    OpenAIRE

    Nieschlag E

    2011-01-01

    Research has established the principle of hormonal male contraception based on suppression of gonadotropins and spermatogenesis. All hormonal male contraceptives use testosterone, but only in East Asian men can testosterone alone suppress spermatogenesis to a level compatible with contraceptive protection. In Caucasians, additional agents are required of which progestins are favored. Clinical trials concentrate on testosterone combined with norethisterone, desogestrel, etonogestrel or depo...

  6. Hormone therapy and different ovarian cancers

    DEFF Research Database (Denmark)

    Mørch, Lina Steinrud; Løkkegaard, Ellen; Andreasen, Anne Helms;

    2012-01-01

    Postmenopausal hormone therapy use increases the risk of ovarian cancer. In the present study, the authors examined the risks of different histologic types of ovarian cancer associated with hormone therapy. Using Danish national registers, the authors identified 909,946 women who were followed fr...

  7. Sex hormones and skeletal muscle weakness

    DEFF Research Database (Denmark)

    Sipilä, Sarianna; Narici, Marco; Kjaer, Michael;

    2013-01-01

    Human ageing is accompanied with deterioration in endocrine functions the most notable and well characterized of which being the decrease in the production of sex hormones. Current research literature suggests that low sex hormone concentration may be among the key mechanism for sarcopenia...

  8. Floral induction, floral hormones and flowering

    NARCIS (Netherlands)

    Pol, van der P.A.

    1972-01-01

    The factors, influencing the synthesis and action of floral hormones, and possible differences between floral hormones in different plants were studied. The experimental results are summarized in the conclusions 1-20, on pages 35-36 (Crassulaceae'); 21-39 on pages 58-59 ('Xanthium strumarium') and 4

  9. Combined Hormonal Birth Control: Pill, Patch, and Ring

    Science.gov (United States)

    ... Gynecologists f AQ FREQUENTLY ASKED QUESTIONS FAQ185 CONTRACEPTION Combined Hormonal Birth Control: Pill, Patch, and Ring • What are combined hormonal birth control methods? • How do combined hormonal ...

  10. Hormonal contraception for human males: prospects

    Institute of Scientific and Technical Information of China (English)

    P.R.K.Reddy

    2000-01-01

    Development of an ideal hormonal contraceptive for man has been the goal of several research workers during the past few decades. Suppression of pituitary gonadotropic hormones, which in turn would inhibit spermatogenesis while maintaining normal libido and potentia has been the approach for a contraceptive agent. Intramuscularly administered and orally active testosterone or testosterone in combination with progesterone have been shown to cause inhibition of spermatogenesis resulting in azoospermia in normal men. Similarly testosterone has been used in combination with gonadotropin releasing hormone antagonists and agonists to inhibit pituitary gonadotropic hormone release. Immunological approach to neutralize the circulating levels of follicle stimulating hormone has also been shown to cause inhibition of spermatogenesis. The available literature shows that testosterone causes reversible azoospermia without any significant side effects in Asian population effectively and appears to be a promising chemical for control of fertility in man.( Asian J Androl 2000 ; 2 : 46 - 50 )

  11. Current Status of Biosimilar Growth Hormone

    Directory of Open Access Journals (Sweden)

    Saenger Paul

    2009-08-01

    Full Text Available As the first wave of biopharmaceuticals is set to expire, biosimilars or follow-on protein products (FOPPs have emerged. The regulatory foundation for these products is more advanced and better codified in Europe than in the US. Recent approval of biosimilar Somatropin (growth hormone in Europe and the US prompted this paper. The scientific viability of biosimilar growth hormone is reviewed. Efficacy and safety data (growth rates, IGF-1 generation for up to 7 years for pediatric indications measure up favorably to previously approved growth hormones as reference comparators. While the approval in the US is currently only for treatment of growth hormone deficiency (GHD in children and adults, the commercial use of approved biosimilar growth hormones will allow in the future for in-depth estimation of their efficacy and safety in non-GH deficient states as well.

  12. Current Status of Biosimilar Growth Hormone

    Directory of Open Access Journals (Sweden)

    Paul Saenger

    2009-01-01

    Full Text Available As the first wave of biopharmaceuticals is set to expire, biosimilars or follow-on protein products (FOPPs have emerged. The regulatory foundation for these products is more advanced and better codified in Europe than in the US. Recent approval of biosimilar Somatropin (growth hormone in Europe and the US prompted this paper. The scientific viability of biosimilar growth hormone is reviewed. Efficacy and safety data (growth rates, IGF-1 generation for up to 7 years for pediatric indications measure up favorably to previously approved growth hormones as reference comparators. While the approval in the US is currently only for treatment of growth hormone deficiency (GHD in children and adults, the commercial use of approved biosimilar growth hormones will allow in the future for in-depth estimation of their efficacy and safety in non-GH deficient states as well.

  13. Hormone therapy and different ovarian cancers

    DEFF Research Database (Denmark)

    Mørch, Lina Steinrud; Løkkegaard, Ellen; Andreasen, Anne Helms;

    2012-01-01

    Postmenopausal hormone therapy use increases the risk of ovarian cancer. In the present study, the authors examined the risks of different histologic types of ovarian cancer associated with hormone therapy. Using Danish national registers, the authors identified 909,946 women who were followed from...... 1995-2005. The women were 50-79 years of age and had no prior hormone-sensitive cancers or bilateral oophorectomy. Hormone therapy prescription data were obtained from the National Register of Medicinal Product Statistics. The National Cancer and Pathology Register provided data on ovarian cancers......, including information about tumor histology. The authors performed Poisson regression analyses that included hormone exposures and confounders as time-dependent covariates. In an average of 8.0 years of follow up, 2,681 cases of epithelial ovarian cancer were detected. Compared with never users, women...

  14. [Plant hormones, plant growth regulators].

    Science.gov (United States)

    Végvári, György; Vidéki, Edina

    2014-06-29

    Plants seem to be rather defenceless, they are unable to do motion, have no nervous system or immune system unlike animals. Besides this, plants do have hormones, though these substances are produced not in glands. In view of their complexity they lagged behind animals, however, plant organisms show large scale integration in their structure and function. In higher plants, such as in animals, the intercellular communication is fulfilled through chemical messengers. These specific compounds in plants are called phytohormones, or in a wide sense, bioregulators. Even a small quantity of these endogenous organic compounds are able to regulate the operation, growth and development of higher plants, and keep the connection between cells, tissues and synergy between organs. Since they do not have nervous and immume systems, phytohormones play essential role in plants' life.

  15. Phosphorylation of chicken growth hormone

    Energy Technology Data Exchange (ETDEWEB)

    Aramburo, C.; Montiel, J.L. (Universidad Nacional Autonoma de Mexico (Mexico)); Donoghue, D.; Scanes, C.G. (Rutgers Univ., New Brunswick, NJ (USA)); Berghman, L.R. (Laboratory for Neuroendocrinology and Immunological Biotechnology, Louvain (Belgium))

    1990-01-01

    The possibility that chicken growth hormone (cGH) can be phosphorylated has been examined. Both native and biosynthetic cGH were phosphorylated by cAMP-dependent protein kinase (and {gamma}-{sup 32}P-ATP). The extent of phosphorylation was however less than that observed with ovine prolactin. Under the conditions employed, glycosylated cGH was not phosphorylated. Chicken anterior pituitary cells in primary culture were incubated in the presence of {sup 32}P-phosphate. Radioactive phosphate was incorporated in vitro into the fraction immunoprecipitable with antisera against cGH. Incorporation was increased with cell number and time of incubation. The presence of GH releasing factor (GRF) increased the release of {sup 32}P-phosphate labeled immunoprecipitable GH into the incubation media but not content of immunoprecipitable GH in the cells. The molecular weight of the phosphorylated immunoreactive cGH in the cells corresponded to cGH dimer.

  16. Hormonal changes in antiorthostatic rats

    Science.gov (United States)

    Popovic, V.; Popovic, P.; Honeycutt, C.

    1982-01-01

    Hypokinesia, especially hypokinesia with negative tilt ('antiorthostatic hypokinesia'), mimics some of the effects of weightlessness. It is shown that cardiac output is increased during early exposure of rats to antiorthostatic hypokinesia. The increase of the stroke volume and of the cardiac output observed in the antiorthostatic hypokinetic rats is probably the consequence of a blood volume shift toward the chest brought forth by head-down positioning of the animals. It is also possible that struggling of the animals to escape from the harness and an increased metabolism contribute to the elevation of cardiac output. In order to study this hypothesis 'stress hormones' were measured in the antiorthostatic rats. Plasma levels of ACTH, corticosterone and prolactin were measured in the arterial blood (0.3 ml) sampled before, during and after hypokinesia from chronic aortic cannulas of the rats.

  17. Adipose tissues and thyroid hormones

    Directory of Open Access Journals (Sweden)

    Maria-Jesus eObregon

    2014-12-01

    Full Text Available The maintenance of energy balance is regulated by complex homeostatic mechanisms, including those emanating from adipose tissue. The main function of the adipose tissue is to store the excess of metabolic energy in the form of fat. The energy stored as fat can be mobilized during periods of energy deprivation (hunger, fasting, diseases. The adipose tissue has also a homeostatic role regulating energy balance and functioning as endocrine organ that secretes substances that control body homeostasis. Two adipose tissues have been identified: white and brown adipose tissues (WAT and BAT with different phenotype, function and regulation. WAT stores energy, while BAT dissipates energy as heat. Brown and white adipocytes have different ontogenetic origin and lineage and specific markers of WAT and BAT have been identified. Brite or beige adipose tissue has been identified in WAT with some properties of BAT. Thyroid hormones exert pleiotropic actions, regulating the differentiation process in many tissues including the adipose tissue. Adipogenesis gives raise to mature adipocytes and is regulated by several transcription factors (c/EBPs, PPARs that coordinately activate specific genes, resulting in the adipocyte phenotype. T3 regulates several genes involved in lipid mobilization and storage and in thermogenesis. Both WAT and BAT are targets of thyroid hormones, which regulate genes crucial for their proper function: lipogenesis, lipolysis, thermogenesis, mitochondrial function, transcription factors, the availability of nutrients. T3 acts directly through specific TREs in the gene promoters, regulating transcription factors. The deiodinases D3, D2 and D1 regulate the availability of T3. D3 is activated during proliferation, while D2 is linked to the adipocyte differentiation program, providing T3 needed for lipogenesis and thermogenesis. We examine the differences between BAT, WAT and brite/beige adipocytes and the process that activate UCP1 in WAT and

  18. Intrauterine sexual differentiation: biosyntesis and action of sexual steroid hormones

    OpenAIRE

    Amilton Cesar dos Santos; Diego Carvalho Viana; Gleidson Benevides de Oliveira; Luis Miguel Lobo; Antônio Chaves Assis-Neto

    2015-01-01

    The objective of this review was to describe sexual differentiation events in mammals, relating them to biosynthesis of sexual steroid hormones and their mechanisms of action. Cholesterol is the precursor of sexual steroid hormone biosynthesis via action of several enzymes converting these hormones. Progestagens hormones serve as substrate for the production of androgens, which in turn serve as substrate for estrogen hormones. These hormones are responsible for sexual differentiation and repr...

  19. Hormonal Factors and Disturbances in Eating Disorders.

    Science.gov (United States)

    Culbert, Kristen M; Racine, Sarah E; Klump, Kelly L

    2016-07-01

    This review summarizes the current state of the literature regarding hormonal correlates of, and etiologic influences on, eating pathology. Several hormones (e.g., ghrelin, CCK, GLP-1, PYY, leptin, oxytocin, cortisol) are disrupted during the ill state of eating disorders and likely contribute to the maintenance of core symptoms (e.g., dietary restriction, binge eating) and/or co-occurring features (e.g., mood symptoms, attentional biases). Some of these hormones (e.g., ghrelin, cortisol) may also be related to eating pathology via links with psychological stress. Despite these effects, the role of hormonal factors in the etiology of eating disorders remains unknown. The strongest evidence for etiologic effects has emerged for ovarian hormones, as changes in ovarian hormones predict changes in phenotypic and genetic influences on disordered eating. Future studies would benefit from utilizing etiologically informative designs (e.g., high risk, behavioral genetic) and continuing to explore factors (e.g., psychological, neural responsivity) that may impact hormonal influences on eating pathology.

  20. Effects of hormones on lipids and lipoproteins

    Energy Technology Data Exchange (ETDEWEB)

    Krauss, R.M.

    1991-12-01

    Levels of plasma lipids and lipoproteins are strong predictors for the development of atherosclerotic cardiovascular disease in postmenopausal women. In women, as in men, numerous factors contribute to variations in plasma lipoproteins that may affect cardiovascular disease risk. These include age, dietary components, adiposity, genetic traits, and hormonal changes. Each of these factors may operate to varying degrees in determining changes in plasma lipoprotein profiles accompanying menopause- Cross-sectional and longitudinal studies have suggested increases in levels of cholesterol, low density lipoproteins (LDL) and triglyceride-rich lipoproteins associated with menopause. High density lipoproteins (HDL), which are higher in women than men and are thought to contribute to relative protection of premenopausal women from cardiovascular disease, remain relatively constant in the years following menopause, although small, and perhaps transient reductions in the HDL{sub 2} subfraction have been reported in relation to reduced estradiol level following menopause. Despite these associations, it has been difficult to determine the role of endogenous hormones in influencing the plasma lipoproteins of postmenopausal women. In principle, the effects of hormone replacement should act to reverse any alterations in lipoprotein metabolism that are due to postmenopausal hormone changes. While there may be beneficial effects on lipoproteins, hormone treatment does not restore a premenopausal lipoprotein profile. Furthermore, it is not dear to what extent exogenous hormone-induced lipoprotein changes contribute to the reduced incidence of cardiovascular disease with hormone replacement therapy.

  1. Hormonal Factors and Disturbances in Eating Disorders.

    Science.gov (United States)

    Culbert, Kristen M; Racine, Sarah E; Klump, Kelly L

    2016-07-01

    This review summarizes the current state of the literature regarding hormonal correlates of, and etiologic influences on, eating pathology. Several hormones (e.g., ghrelin, CCK, GLP-1, PYY, leptin, oxytocin, cortisol) are disrupted during the ill state of eating disorders and likely contribute to the maintenance of core symptoms (e.g., dietary restriction, binge eating) and/or co-occurring features (e.g., mood symptoms, attentional biases). Some of these hormones (e.g., ghrelin, cortisol) may also be related to eating pathology via links with psychological stress. Despite these effects, the role of hormonal factors in the etiology of eating disorders remains unknown. The strongest evidence for etiologic effects has emerged for ovarian hormones, as changes in ovarian hormones predict changes in phenotypic and genetic influences on disordered eating. Future studies would benefit from utilizing etiologically informative designs (e.g., high risk, behavioral genetic) and continuing to explore factors (e.g., psychological, neural responsivity) that may impact hormonal influences on eating pathology. PMID:27222139

  2. Arabidopsis Hormone Database: a comprehensive genetic and phenotypic information database for plant hormone research in Arabidopsis.

    Science.gov (United States)

    Peng, Zhi-yu; Zhou, Xin; Li, Linchuan; Yu, Xiangchun; Li, Hongjiang; Jiang, Zhiqiang; Cao, Guangyu; Bai, Mingyi; Wang, Xingchun; Jiang, Caifu; Lu, Haibin; Hou, Xianhui; Qu, Lijia; Wang, Zhiyong; Zuo, Jianru; Fu, Xiangdong; Su, Zhen; Li, Songgang; Guo, Hongwei

    2009-01-01

    Plant hormones are small organic molecules that influence almost every aspect of plant growth and development. Genetic and molecular studies have revealed a large number of genes that are involved in responses to numerous plant hormones, including auxin, gibberellin, cytokinin, abscisic acid, ethylene, jasmonic acid, salicylic acid, and brassinosteroid. Here, we develop an Arabidopsis hormone database, which aims to provide a systematic and comprehensive view of genes participating in plant hormonal regulation, as well as morphological phenotypes controlled by plant hormones. Based on data from mutant studies, transgenic analysis and gene ontology (GO) annotation, we have identified a total of 1026 genes in the Arabidopsis genome that participate in plant hormone functions. Meanwhile, a phenotype ontology is developed to precisely describe myriad hormone-regulated morphological processes with standardized vocabularies. A web interface (http://ahd.cbi.pku.edu.cn) would allow users to quickly get access to information about these hormone-related genes, including sequences, functional category, mutant information, phenotypic description, microarray data and linked publications. Several applications of this database in studying plant hormonal regulation and hormone cross-talk will be presented and discussed. PMID:19015126

  3. Mechanisms of genotoxic effects of hormones

    Directory of Open Access Journals (Sweden)

    Đelić Ninoslav J.

    2002-01-01

    Full Text Available A concept that compounds commonly present in biological systems lack genotoxic and mutagenic activities is generally in use, hence a low number of endogenous substances have ever been tested to mutagenicity. Epidemiological and experimental analyses indicated, however, that sexual steroids could contribute to initiation and/or continuation of malign diseases. Detailed studies using methods of biochemistry, molecular biology, cytogenetics and other branches, showed that not only epigenetic mechanisms, such as a stimulation of cell proliferation, but also certain hormones, that can express genotoxic effects, such as covalent DNA modification, then chromosomal lesions and chromosomal aberrations, are in the background of malign transformation under activities of hormones. In the case of oestrogens, it was shown that excessive hormonal stimulation led to a metabolic conversion of these hormones to reactive intermediates with formation of reactive oxygenic derivates, so that cells were virtually under conditions of oxidative stress. Individual and tissue susceptibility to occurrence of deterioration of DNA and other cell components generally results from the differences in efficiency of enzymic and non-enzymic mechanisms of resistance against oxidative stress. Besides, steroid thyeroid hormones and catecholamine (dopamine, noradrenaline/norepinephrine and adrenaline can express genotoxic effects in some test-systems. It is interesting that all above mentioned hormones have a phenolic group. Data on possible genotoxic effects of peptide and protein hormones are very scarce, but based on the available literature it is considered that this group of hormones probably lacks mutagenic activities. The possibility that hormones, as endogenous substances, express mutagenic activities results from the fact that DNA is, regardless of chemical and metabolic stability susceptible, to a certain extent, to changeability compatible with the processes of the

  4. Pituitary and placental hormone levels in pseudocyesis.

    Science.gov (United States)

    Osotimehin, B O; Ladipo, O A; Adejuwon, C A; Otolorin, E O

    1981-10-01

    Twelve patients with clinical features of pseudocyesis were divided into two groups according to the presence or absence of galactorrhea. The mean serum prolactin level of patients with galactorrhea was significantly higher than the normal values of the patients without galactorrhea. The mean serum levels of luteinizing hormone and follicle-stimulating hormone were markedly elevated in patients without galactorrhea. This was especially true of luteinizing hormone. Serum levels of human chorionic gonadotropin were undetectable in all patients. The significance of these observations is discussed.

  5. Hot stuff: thyroid hormones and AMPK

    Institute of Scientific and Technical Information of China (English)

    D Grahame Hardie

    2010-01-01

    @@ Every high school biology student is taught that thyroid hormones increase the metabolic rate. This conclusion mainly arose from the effects of hyperthyroidism, the clinical condition characterized by excessive production of the hormones. Symptoms include weight loss despite increased appetite, tremors,cardiac palpitations, irritability, intolerance to heat and increased perspiration.Although understanding of how thyroid hormones increase metabolic rate at the molecular level has been elusive,a recent paper by Antonio Vidal-Puig and colleagues in Nature Medicine [ 1 ]provides important new insights.

  6. The Radioimmunoassay of Fluid and Electrolyte Hormones

    Science.gov (United States)

    Keil, Lanny C.

    1985-01-01

    The subject of the paper will be the assay of fluid/electrolyte hormones. ADH (antidiuretic hormone also referred to as vasopressin) reduces fluid loss by increasing water reabsorption by the kidney. The stimuli for its release from the pituitary are loss of blood, dehydration, or increased salt intake. Angiotensin II is the next hormone of interest. It is "generated" from a blood protein by the release of renin from the kidney. One of its functions is to stimulate the secretion of aldosterone from the adrenal gland. Release of renin is also stimulated by volume and sodium loss.

  7. Impact of Growth Hormone on Cystatin C

    OpenAIRE

    Lisa Sze; René L. Bernays; Cornelia Zwimpfer; Peter Wiesli; Michael Brändle; Christoph Schmid

    2013-01-01

    Background: Cystatin C (CysC) is an alternative marker to creatinine for estimation of the glomerular filtration rate (GFR). Hormones such as thyroid hormones and glucocorticoids are known to have an impact on CysC. In this study, we examined the effect of growth hormone (GH) on CysC in patients with acromegaly undergoing transsphenoidal surgery. Methods: Creatinine, CysC, GH and insulin-like growth factor-1 (IGF-1) were determined in 24 patients with acromegaly before and following transsphe...

  8. Impact of Growth Hormone on Cystatin C

    OpenAIRE

    Sze, Lisa; René L. Bernays; Zwimpfer, Cornelia; Wiesli, Peter; Brändle, Michael; Schmid, Christoph

    2013-01-01

    BACKGROUND: Cystatin C (CysC) is an alternative marker to creatinine for estimation of the glomerular filtration rate (GFR). Hormones such as thyroid hormones and glucocorticoids are known to have an impact on CysC. In this study, we examined the effect of growth hormone (GH) on CysC in patients with acromegaly undergoing transsphenoidal surgery. METHODS: Creatinine, CysC, GH and insulin-like growth factor-1 (IGF-1) were determined in 24 patients with acromegaly before and following transs...

  9. Growth hormone insensitivity syndrome: unusual oral manifestations.

    Science.gov (United States)

    Borges, Alvaro Henrique; Siqueira, Carlos Rodrigo Barros; Pedro, Fábio Luis Miranda; Palma, Vinícius Canavarros; Sakai, Vivien Thiemy; Volpato, Luiz Evaristo Ricci

    2013-01-01

    Children with significant growth retardation and normal levels of growth hormone are diagnosed with growth hormone insensitivity. The main oral findings observed in patients with growth hormone insensitivity syndrome (GHIS) are underdeveloped jaws, crowded teeth and delayed eruption of permanent teeth. This manuscript describes a 9-year-old child diagnosed with GHIS, who had delayed eruption of permanent teeth and 14 unerupted supernumerary teeth. All supernumerary teeth were extracted except for two maxillary and one mandibular teeth which were difficult to identify and access. Multiple supernumerary teeth have never been reported before in patients with GHIS.

  10. Positioning the nodule, the hormone dictum.

    Science.gov (United States)

    Ding, Yiliang; Oldroyd, Giles E D

    2009-02-01

    The formation of a nitrogen-fixing nodule involves two diverse developmental processes in the legume root: infection thread initiation in epidermal cells and nodule primordia formation in the cortex. Several plant hormones have been reported to positively or negatively regulate nodulation. These hormones function at different stages in the nodulation process and may facilitate the coordinated development of the epidermal and cortical developmental programs that are necessary to allow bacterial infection into the developing nodule. In this paper, we review and discuss how the tissue specific nature of hormonal action dictates where, when and how a nodule is formed. PMID:19649179

  11. Hormonal component of tumor photodynamic therapy response

    Science.gov (United States)

    Korbelik, Mladen; Merchant, Soroush

    2008-02-01

    The involvement of adrenal glucocorticoid hormones in the response of the treatment of solid tumors by photodynamic therapy (PDT) comes from the induction of acute phase response by this modality. This adrenal gland activity is orchestrated through the engagement of the hypothalamic-pituitary-adrenal hormonal axis incited by stress signals emanating from the PDT-treated tumor. Glucocorticoid hormone activity engendered within the context of PDT-induced acute phase response performs multiple important functions; among other involvements they beget acute phase reactant production, systemic neutrophil mobilization, and control the production of inflammation-modulating and immunoregulatory proteins.

  12. Hormonal contraception and platelet function.

    Science.gov (United States)

    Saleh, A A; Ginsburg, K A; Duchon, T A; Dorey, L G; Hirata, J; Alshameeri, R S; Dombrowski, M P; Mammen, E F

    1995-05-15

    73 healthy women (29 controls, 25 using OCs, and 19 using Norplant) were selected from the clinic population at North Oakland Medical Center for inclusion in this study after obtaining informed consent. Age, race, height, weight, blood pressure, and cigarette smoking were recorded for each subject. 12 patients were on monophasic OCs while 13 were on triphasic preparations. Both hormonal contraceptive groups had used their particular contraceptive for at least 3 months prior to blood drawing. Platelet tests were performed within 2 hours of sample collection: platelet counts (PLC) and mean platelet volume (MPV) were determined on an Automated Platelet Counter (Baker 810 Platelet Analyzer). Whole blood aggregation was performed on a platelet aggregometer (Chrono-Log, Model 550) using both ADP (ADP, 5 mM) and collagen (COLL, 2 mcg/ml) as inducing agents. Demographic differences were not significant (p 0.05) among the 3 treatment groups, whose average age was 25.3-25.8 years old. Furthermore, no significant differences (p 0.05) in platelet function were detected among controls or subjects receiving either oral contraceptives or Norplant, compared to control patients. The mean platelet counts (X 10/9/L) were 223 for OC users, 231 for Norplant users, and 232 for controls. The respective platelet aggregation (ADP, ohms) values were 12.5, 18.0, and 19.2 as well as (COLL, ohms) 35.6, 40.7, and 39.0. These results demonstrated that there is no evidence for altered platelet function, with the testing methods employed, in women using either Norplant or combination low dose oral contraceptives. To date, several studies have examined this issue, with contradictory reports about the effects of hormonal contraceptives in platelet function. After controlling for differences between various steroid preparations and other such confounding variables, some of these conflicting conclusions could be the result of a lack of uniformity among the methods used to evaluate platelet aggregation

  13. Oxytocin is a cardiovascular hormone

    Directory of Open Access Journals (Sweden)

    Gutkowska J.

    2000-01-01

    Full Text Available Oxytocin (OT, a nonapeptide, was the first hormone to have its biological activities established and chemical structure determined. It was believed that OT is released from hypothalamic nerve terminals of the posterior hypophysis into the circulation where it stimulates uterine contractions during parturition, and milk ejection during lactation. However, equivalent concentrations of OT were found in the male hypophysis, and similar stimuli of OT release were determined for both sexes, suggesting other physiological functions. Indeed, recent studies indicate that OT is involved in cognition, tolerance, adaptation and complex sexual and maternal behaviour, as well as in the regulation of cardiovascular functions. It has long been known that OT induces natriuresis and causes a fall in mean arterial pressure, both after acute and chronic treatment, but the mechanism was not clear. The discovery of the natriuretic family shed new light on this matter. Atrial natriuretic peptide (ANP, a potent natriuretic and vasorelaxant hormone, originally isolated from rat atria, has been found at other sites, including the brain. Blood volume expansion causes ANP release that is believed to be important in the induction of natriuresis and diuresis, which in turn act to reduce the increase in blood volume. Neurohypophysectomy totally abolishes the ANP response to volume expansion. This indicates that one of the major hypophyseal peptides is responsible for ANP release. The role of ANP in OT-induced natriuresis was evaluated, and we hypothesized that the cardio-renal effects of OT are mediated by the release of ANP from the heart. To support this hypothesis, we have demonstrated the presence and synthesis of OT receptors in all heart compartments and the vasculature. The functionality of these receptors has been established by the ability of OT to induce ANP release from perfused heart or atrial slices. Furthermore, we have shown that the heart and large vessels

  14. Hormônio anti-Mülleriano sérico para predição da resposta ovariana em ciclos de reprodução assistida Serum anti-Müllerian hormone to predict ovarian response in assisted reproduction cycles

    Directory of Open Access Journals (Sweden)

    Gustavo Salata Romão

    2012-12-01

    Full Text Available OBJETIVOS: Comparar as concentrações séricas do hormônio anti- Mülleriano (AMH no sétimo dia de estimulação ovariana em pacientes boas e más respondedoras. MÉTODOS: Foram incluídas 19 mulheres com idade ≥35 anos, ciclos menstruais regulares e que foram submetidas à estimulação ovariana para reprodução assistida. Foram excluídas mulheres portadoras de endometriose ou síndrome dos ovários policísticos ou aquelas submetidas previamente à cirurgia ovariana. Foram coletadas amostras de sangue periférico no dia basal e no sétimo dia de estimulação para dosagens de AMH, hormônio folículo estimulante (FSH e estradiol. Os níveis de AMH foram avaliados pelo método de enzimoimunoensaio (ELISA e os níveis de FSH e estradiol foram avaliados por imunoquimioluminescência. Ao final do ciclo, as pacientes foram classificadas como normo (obtenção de quatro ou mais oócitos durante a captação ou más respondedoras (obtenção de menos de quatro oócitos ou cancelamento do ciclo por má resposta e analisadas comparativamente em relação aos níveis hormonais, duração da estimulação ovariana, número de folículos recrutados, embriões produzidos e transferidos através do teste t. A associação entre os níveis de AMH e os parâmetros acima também foi avaliada pelo teste de correlação de Spearman. RESULTADOS: Não houve diferença significativa entre os grupos para os níveis de AMH, FSH e estradiol no dia basal e no sétimo dia de estimulação ovariana, sendo observada correlação significativa entre os níveis de AMH do sétimo dia e a quantidade total de FSH exógeno utilizada (p=0,02. CONCLUSÕES: Os níveis de AMH obtidos no sétimo dia do ciclo de estimulação ovariana não parecem predizer o padrão de resposta ovariana, não sendo recomendadas as suas dosagens para esta finalidade.PURPOSE: To compare serum anti-Mullerian hormone (AMH levels on the seventh day of ovarian stimulation between normal and poor

  15. Leptin:a multifunctional hormone

    Institute of Scientific and Technical Information of China (English)

    HUANGLU; CAILI

    2000-01-01

    Leptin is the protein product encoded by the obese(ob) gene.It is a circulating hormone produced primarily by the adipose tissue.ob/ob mice with mutations of the gene encoding leptin become morbidly obese,infertile,hyperphagic,hypothermic,and diabetic.Since the cloning of leptin in 1994,our knowledge in body weight regulation and the role played by leptin has increased substantially.We now know that leptin signals through its receptor,OB-R,which is a member of the cytokine receptor superfamily.Leptin serves as an adiposity signal to inform the brain the adipose tissue mass in a negative feedback loop regulating food intake and energy expenditure.Leptin also plays important roles in angiogenesis,immune function,fertility,and bone formation.Humans with mutations in the gene encoding leptin are also morbidly obese and respond to leptin treatment,demonstrating that enhancing or inhibiting leptin's activities in vivo may have potential therapeutic benefits.

  16. Growth hormone doping: a review

    Directory of Open Access Journals (Sweden)

    Erotokritou-Mulligan I

    2011-07-01

    Full Text Available Ioulietta Erotokritou-Mulligan, Richard IG Holt, Peter H SönksenDevelopmental Origins of Health and Disease Division, University of Southampton School of Medicine, The Institute of Developmental Science, Southampton General Hospital, Southampton, UKAbstract: The use of growth hormone (GH as a performance enhancing substance was first promoted in lay publications, long before scientists fully acknowledged its benefits. It is thought athletes currently use GH to enhance their athletic performance and to accelerate the healing of sporting injuries. Over recent years, a number of high profile athletes have admitted to using GH. To date, there is only limited and weak evidence for its beneficial effects on performance. Nevertheless the “hype” around its effectiveness and the lack of a foolproof detection methodology that will detect its abuse longer than 24 hours after the last injection has encouraged its widespread use. This article reviews the current evidence of the ergogenic effects of GH along with the risks associated with its use. The review also examines methodologies, both currently available and in development for detecting its abuse.Keywords: performance enhancing substance, GH, doping in sport, detection methods

  17. Thyroid hormones and renin secretion.

    Science.gov (United States)

    Ganong, W F

    Circulating angiotensin is produced by the action of renin from the kidneys on circulating angiotensinogen. There are other renin-angiotensin systems in various organs in the body, and recent observations raise the intriguing possibility that angiotensin II is produced by a totally intracellular pathway in the juxtaglomerular cells, the gonadotrops of the anterior pituitary, neurons, in the brain, salivary duct cells, and neuroblastoma cells. Circulating angiotensin II levels depend in large part on the plasma concentration of angiotensinogen, which is hormonally regulated, and on the rate of renin secretion. Renin secretion is regulated by an intrarenal baroreceptor mechanism, a macula densa mechanism, angiotensin II, vasopressin, and the sympathetic nervous system. The increase in renin secretion produced by sympathetic discharge is mediated for the most part by beta-adrenergic receptors, which are probably located on the juxtaglomerular cells. Hyperthyroidism would be expected to be associated with increased renin secretion in view of the increased beta-adrenergic activity in this condition, and hypothyroidism would be associated with decreased plasma renin activity due to decreased beta-adrenergic activity. Our recent research on serotonin-mediated increases in renin secretion that depend on the integrity of the dorsal raphe nucleus and the mediobasal hypothalamus has led us to investigate the effect of the pituitary on the renin response to p-chloroamphetamine. The response is potentiated immediately after hypophysectomy, but 22 days after the operation, it is abolished. This slowly developing decrease in responsiveness may be due to decreased thyroid function.

  18. Hormone therapy use, sex hormone concentrations and gene expression : The Norwegian Women and Cancer study (NOWAC)

    OpenAIRE

    Waaseth, Marit

    2010-01-01

    According to sales statistics, the use of hormone therapy (HT) increased markedly in Norway through the 1990s, but decreased from 2002. Both endogenous and exogenous sex hormones are known risk factors for cancer among women. Cancer is characterized by uncontrolled cell growth which develops gradually through genomic alterations. Technological developments provide the opportunity to investigate relationships between sex hormones and blood gene expression in a population based cohort like the ...

  19. Arabidopsis Hormone Database: a comprehensive genetic and phenotypic information database for plant hormone research in Arabidopsis

    OpenAIRE

    Peng, Zhi-Yu; Zhou, Xin; Li, Linchuan; Yu, Xiangchun; Li, Hongjiang; Jiang, Zhiqiang; Cao, Guangyu; Bai, Mingyi; Wang, Xingchun; Jiang, Caifu; Lu, Haibin; Hou, Xianhui; Qu, Lijia; Wang, Zhiyong; Zuo, Jianru

    2008-01-01

    Plant hormones are small organic molecules that influence almost every aspect of plant growth and development. Genetic and molecular studies have revealed a large number of genes that are involved in responses to numerous plant hormones, including auxin, gibberellin, cytokinin, abscisic acid, ethylene, jasmonic acid, salicylic acid, and brassinosteroid. Here, we develop an Arabidopsis hormone database, which aims to provide a systematic and comprehensive view of genes participating in plant h...

  20. FSH (Follicle-Stimulating Hormone) Test

    Science.gov (United States)

    ... youth within this age range. Some of the causes for delayed puberty can include: Dysfunction of the ovaries or testicles Hormone deficiency Turner syndrome Klinefelter syndrome Chronic infections Cancer Eating disorder (anorexia nervosa) ^ Back to top Is there anything ...

  1. Menopausal hormone use and ovarian cancer risk

    DEFF Research Database (Denmark)

    Beral, V; Gaitskell, K; Hermon, C;

    2015-01-01

    BACKGROUND: Half the epidemiological studies with information about menopausal hormone therapy and ovarian cancer risk remain unpublished, and some retrospective studies could have been biased by selective participation or recall. We aimed to assess with minimal bias the effects of hormone therapy...... on ovarian cancer risk. METHODS: Individual participant datasets from 52 epidemiological studies were analysed centrally. The principal analyses involved the prospective studies (with last hormone therapy use extrapolated forwards for up to 4 years). Sensitivity analyses included the retrospective studies....... Adjusted Poisson regressions yielded relative risks (RRs) versus never-use. FINDINGS: During prospective follow-up, 12 110 postmenopausal women, 55% (6601) of whom had used hormone therapy, developed ovarian cancer. Among women last recorded as current users, risk was increased even with

  2. Interpretation of growth hormone provocative tests

    DEFF Research Database (Denmark)

    Andersson, A M; Orskov, H; Ranke, M B;

    1995-01-01

    To compare interpretations of growth hormone (GH) provocative tests in laboratories using six different GH immunoassays (one enzymeimmunometric assay (EIMA, assay 1), one immunoradiometric assay (IRMA, assay 5), one time-resolved fluorimmunometric assay (TRFIA, assay 3) and three radioimmunoassays...

  3. Sulfation of thyroid hormone by estrogen sulfotransferase

    NARCIS (Netherlands)

    M.H.A. Kester (Monique); T.J. Visser (Ton); C.H. van Dijk (Caren); D. Tibboel (Dick); A.M. Hood (Margaret); N.J. Rose; W. Meinl; U. Pabel; H. Glatt; C.N. Falany; M.W. Coughtrie

    1999-01-01

    textabstractSulfation is one of the pathways by which thyroid hormone is inactivated. Iodothyronine sulfate concentrations are very high in human fetal blood and amniotic fluid, suggesting important production of these conjugates in utero. Human estrogen sulfotransferas

  4. TSH (Thyroid-Stimulating Hormone) Test

    Science.gov (United States)

    ... problem with the pituitary gland , such as a tumor producing unregulated levels of TSH A low TSH result may indicate: An overactive thyroid gland (hyperthyroidism) Excessive amounts of thyroid hormone medication in ...

  5. Growth hormone and selective attention : A review

    NARCIS (Netherlands)

    Quik, Elise H.; van Dam, P. Sytze; Kenemans, J. Leon

    2010-01-01

    Introduction: The relation between growth hormone (GH) secretion and general cognitive function has been established. General cognitive functioning depends on core functions including selective attention, which have not been addressed specifically in relation to GH. The present review addresses curr

  6. Management of Hormone Deprivation Symptoms After Cancer.

    Science.gov (United States)

    Faubion, Stephanie S; Loprinzi, Charles L; Ruddy, Kathryn J

    2016-08-01

    Cancer survivors often experience symptoms related to hormone deprivation, including vasomotor symptoms, genitourinary symptoms, and sexual health concerns. These symptoms can occur due to natural menopause in midlife women, or they can be brought on by oncologic therapies in younger women or men. We searched PubMed for English-language studies from January 1990 through January 2016 to identify relevant articles on the management of hormone deprivation symptoms, including vasomotor, genitourinary, and sexual symptoms in patients with cancer. The search terms used included hormone deprivation, vasomotor symptoms, hot flash, vaginal dryness, sexual dysfunction, and breast cancer. This manuscript provides a comprehensive description of data supporting the treatment of symptoms associated with hormone deprivation. PMID:27492917

  7. Postmenopausal hormone replacement therapy--clinical implications

    DEFF Research Database (Denmark)

    Ravn, S H; Rosenberg, J; Bostofte, E

    1994-01-01

    in the urogenital tract. Women at risk of osteoporosis will benefit from hormone replacement therapy. The treatment should start as soon after menopause as possible and it is possible that it should be maintained for life. The treatment may be supplemented with extra calcium intake, vitamin D, and maybe calcitonin....... Physical activity should be promoted, and cigarette smoking reduced if possible. Women at risk of cardiovascular disease will also benefit from hormone replacement therapy. There is overwhelming evidence that hormone therapy will protect against both coronary heart disease and stroke......, and there is no increased risk of venous thrombosis or hypertension. A disadvantage of hormone replacement therapy is an increased risk of forming gall-bladder stones and undergoing cholecystectomy. Unopposed estrogen therapy gives a higher incidence of endometrial cancer in women with an intact uterus...

  8. Association of Hormonal Contraception With Depression

    DEFF Research Database (Denmark)

    Skovlund, Charlotte Wessel; Mørch, Lina Steinrud; Kessing, Lars Vedel;

    2016-01-01

    of combined oral contraceptives had an RR of first use of an antidepressant of 1.23 (95% CI, 1.22-1.25). Users of progestogen-only pills had an RR for first use of an antidepressant of 1.34 (95% CI, 1.27-1.40); users of a patch (norgestrolmin), 2.0 (95% CI, 1.76-2.18); users of a vaginal ring (etonogestrel......Importance: Millions of women worldwide use hormonal contraception. Despite the clinical evidence of an influence of hormonal contraception on some women's mood, associations between the use of hormonal contraception and mood disturbances remain inadequately addressed. Objective: To investigate...... whether the use of hormonal contraception is positively associated with subsequent use of antidepressants and a diagnosis of depression at a psychiatric hospital. Design, Setting, and Participants: This nationwide prospective cohort study combined data from the National Prescription Register...

  9. Strategies for the Determination of Plant Hormones.

    Science.gov (United States)

    Davis, Gregory C.; And Others

    1985-01-01

    Describes methods for isolating, purifying, and analyzing plant hormones (molecules involved in plant growth regulation and development). The presentation reflects the historical development of analyses, beginning with bioassays and ending with novel immunochemical assays. (JN)

  10. Innovations in classical hormonal targets for endometriosis.

    Science.gov (United States)

    Pluchino, Nicola; Freschi, Letizia; Wenger, Jean-Marie; Streuli, Isabelle

    2016-01-01

    Endometriosis is a chronic disease of unknown etiology that affects approximately 10% of women in reproductive age. Several evidences show that endometriosis lesions are associated to hormonal imbalance, including estrogen synthesis, metabolism and responsiveness and progesterone resistance. These hormonal alterations influence the ability of endometrial cells to proliferate, migrate and to infiltrate the mesothelium, causing inflammation, pain and infertility. Hormonal imbalance in endometriosis represents also a target for treatment. We provide an overview on therapeutic strategies based on innovations of classical hormonal mechanisms involved in the development of endometriosis lesions. The development phase of new molecules targeting these pathways is also discussed. Endometriosis is a chronic disease involving young women and additional biological targets of estrogen and progesterone pharmacological manipulation (brain, bone and cardiovascular tissue) need to be carefully considered in order to improve and overcome current limits of long-term medical management of endometriosis.

  11. Adaptive diversity: hormones and metabolism in freshwaters.

    Science.gov (United States)

    Laudet, Vincent

    2010-12-01

    Genes underlying the evolution of morphological traits have recently been identified in a number of model species. In the stickleback, the metabolic adaptations to a freshwater habitat have now been linked to a well-known hormonal system. PMID:21145015

  12. Pathology of sleep, hormones and depression

    NARCIS (Netherlands)

    Steiger, A.; Dresler, M.; Kluge, M.; Schussler, P.

    2013-01-01

    In patients with depression, characteristic changes of sleep electroencephalogram and nocturnal hormone secretion occur including rapid eye movement (REM) sleep disinhibition, reduced non-REM sleep and impaired sleep continuity. Neuropeptides are common regulators of the sleep electroencephalogram (

  13. Magnesium and anabolic hormones in older men

    OpenAIRE

    Maggio, M.; Ceda, G.P.; F. Lauretani; Cattabiani, C.; Avantaggiato, E.; Morganti, S.; Ablondi, F.; Bandinelli, S.; Dominguez, L. J.; M. Barbagallo; Paolisso, G.; Semba, R D; Ferrucci, L.

    2011-01-01

    Optimal nutritional and hormonal statuses are determinants of successful ageing. The age associated decline in anabolic hormones such as testosterone and insulin-like growth factor 1 (IGF-1) is a strong predictor of metabolic syndrome, diabetes and mortality in older men. Studies have shown that magnesium intake affects the secretion of total IGF-1 and increase testosterone bioactivity. This observation suggests that magnesium can be a modulator of the anabolic/catabolic equilibrium disrupted...

  14. Studies on the radioimmunoassay of thyroid hormones

    International Nuclear Information System (INIS)

    To establish radioimmunoassay (RIA) systems of 3,5,3'-triiodo-L-thyronine (T3) and thyroxine (T4), various experiments such as 125I labelling, antibody raising, preparation of hormone-free sera and efficient separations of the free hormones from those of antibody bound etc. were conducted. By optimizing many factors, assay systems were successfully established. Some detailed methodological aspects were described. (author)

  15. Nanofiltration of hormone mimicking trace organic contaminants

    OpenAIRE

    Nghiem, D.L.; Schaefer, Andrea; Elimelech, M.

    2005-01-01

    The removal mechanisms of three hormone mimicking organic compounds by nanofiltration (NF) membranes have been examined. Two NF membranes having different pore size were used in laboratory-scale nanofiltration experiments with feed solutions spiked with a hormone mimicking compound ¾ nonylphenol, tert-butyl phenol, or bisphenol A. Retention of the compounds was determined at various solution chemistries, namely aqueous solution pH, ionic strength, and presence of natural organi...

  16. Proinsulin: from hormonal precursor to neuroprotective factor

    OpenAIRE

    Flora de Pablo

    2011-01-01

    In the last decade, non-canonical functions have been described for several molecules with hormone-like activities in different stages of vertebrate development. Since its purification in the 1960s, proinsulin has been one of the best described hormonal precursors, though it has been overwhelmingly studied in the context of insulin, the mature protein secreted by the pancreas. Beginning with our discovery of the presence and precise regulation of proinsulin mRNA in early neurulation and neuro...

  17. Proinsulin: From Hormonal Precursor to Neuroprotective Factor

    OpenAIRE

    De La Rosa, Enrique J; Pablo, Flora de

    2011-01-01

    In the last decade, non-canonical functions have been described for several molecules with hormone-like activities in different stages of vertebrate development. Since its purification in the 1960s, proinsulin has been one of the best described hormonal precursors, though it has been overwhelmingly studied in the context of insulin, the mature protein secreted by the pancreas. Beginning with our discovery of the presence and precise regulation of proinsulin mRNA in early neurulation and neuro...

  18. Hormonal activity in clinically silent adrenal incidentalomas

    OpenAIRE

    Babińska, Anna; Siekierska-Hellmann, Małgorzata; Błaut, Krzysztof; Lewczuk, Anna; Wiśniewski, Piotr; Gnacińska, Maria; Obołończyk, Łukasz; Świątkowska-Stodulska, Renata; Sworczak, Krzysztof

    2012-01-01

    Introduction The rapid development of modern imaging techniques, has led to an increase in accidentally discovered adrenal masses without clinically apparent hormonal abnormalities. Such tumours have been termed “incidentalomas”. The diagnostic work-up in patients with adrenal incidentalomas is aimed at the determination of hormonal activity of the tumour and identification of patients with potentially malignant tumours. The aim of our study was a retrospective analysis of selected clinical c...

  19. Links between growth hormone and aging

    OpenAIRE

    Bartke, Andrzej; Westbrook, Reyhan; Sun, Liou; Ratajczak, Mariusz

    2013-01-01

    Studies in mutant, gene knock-out and transgenic mice demonstrated that growth hormone (GH) signaling has major impact on aging and longevity. Growth hormone-resistant and GH-deficient animals live much longer than their normal siblings, while transgenic mice overexpressing GH are short lived. Actions of GH in juvenile animals appear to be particularly important for life extension and responsible for various phenotypic characteristics of long-lived hypopituitary mutants.

  20. Detecting growth hormone misuse in athletes

    OpenAIRE

    Holt, Richard I.G.

    2013-01-01

    Athletes have been misusing growth hormone (GH) for its anabolic and metabolic effects since the early 1980s, at least a decade before endocrinologists began to treat adults with GH deficiency. Although there is an ongoing debate about whether GH is performance enhancing, recent studies suggest that GH improves strength and sprint capacity, particularly when combined with anabolic steroids. The detection of GH misuse is challenging because it is an endogenous hormone. Two approaches have been...

  1. Expression of Thyroid Hormone Transporters in the Human Hypothalamus

    NARCIS (Netherlands)

    A. Alkemade; E.C.H. Friesema; A. Kalsbeek; D.F. Swaab; T.J. Visser; E. Fliers

    2011-01-01

    Context: Transport of thyroid hormone across the plasma membrane is required for proper thyroid hormone action and metabolism. Several specific thyroid hormone transporters have been identified capable of facilitating uptake and/or efflux of thyroid hormones. Monocarboxylate transporter (MCT)-8, MCT

  2. Intermittent versus continuous administration of growth hormone treatment.

    OpenAIRE

    Hakeem, V; Hindmarsh, P. C.; Brook, C G

    1993-01-01

    Growth hormone treatment given by daily injection was compared with growth hormone given for three weeks of every four. All children had received recombinant human growth hormone for two years before randomisation. Growth velocity decreased in both groups in years one and two of the study but the effect was significantly greater in the group receiving intermittent growth hormone.

  3. Regulation of Thyroid Hormone Bioactivity in Health and Disease

    NARCIS (Netherlands)

    R.P. Peeters (Robin)

    2005-01-01

    textabstractTThyroid hormone plays an essential role in a variety of metabolic processes in the human body. Examples are the effects of thyroid hormone on metabolism and on the heart. The production of thyroid hormone by the thyroid is regulated by thyroid stimulating hormone (TSH) via the TSH re

  4. Overnight Levels of Luteinizing Hormone, Follicle-Stimulating Hormone and Growth Hormone before and during Gonadotropin-Releasing Hormone Analogue Treatment in Short Boys Born Small for Gestational Age

    NARCIS (Netherlands)

    van der Kaay, Danielle C. M.; de Jong, Frank H.; Rose, Susan R.; Odink, Roelof J. H.; Bakker-van Waarde, Willie M.; Sulkers, Eric J.; Hokken-Koelega, Anita C. S.

    2009-01-01

    Aims: To evaluate if 3 months of gonadotropin-releasing hormone analogue (GnRHa) treatment results in sufficient suppression of pubertal luteinizing hormone (LH) and follicle-stimulating hormone (FSH) profile patterns in short pubertal small for gestational age (SGA) boys. To compare growth hormone

  5. Incretin hormone secretion over the day

    DEFF Research Database (Denmark)

    Ahren, B; Carr, RD; Deacon, Carolyn F.

    2010-01-01

    The two incretin hormones glucose-dependent insulinotropic polypeptide (GIP) and glucagon-like peptide-1 (GLP-1) are key factors in the regulation of islet function and glucose metabolism, and incretin-based therapy for type 2 diabetes has gained considerable interest during recent years. Regulat......The two incretin hormones glucose-dependent insulinotropic polypeptide (GIP) and glucagon-like peptide-1 (GLP-1) are key factors in the regulation of islet function and glucose metabolism, and incretin-based therapy for type 2 diabetes has gained considerable interest during recent years....... Regulation of incretin hormone secretion is less well characterized. The main stimulus for incretin hormone secretion is presence of nutrients in the intestinal lumen, and carbohydrate, fat as well as protein all have the capacity to stimulate GIP and GLP-1 secretion. More recently, it has been established...... that a diurnal regulation exists with incretin hormone secretion to an identical meal being greater when the meal is served in the morning compared to in the afternoon. Finally, whether incretin hormone secretion is altered in disease states is an area with, so far, controversial results in different studies...

  6. Sex hormones and the dry eye.

    Science.gov (United States)

    Truong, Susan; Cole, Nerida; Stapleton, Fiona; Golebiowski, Blanka

    2014-07-01

    The greater prevalence of dry eye in women compared to men suggests that sex hormones may have a role in this condition. This review aims to present evidence for how sex hormones may affect the ocular structures involved in the production, regulation and maintenance of the normal tear film. It is hypothesised that hormone changes alter the homeostasis of the ocular surface and contribute to dry eye. Androgens impact on the structure and function of the meibomian and lacrimal glands and therefore androgen deficiency is, at least in part, associated with the aetiology of dry eye. In contrast, reports of the effects of oestrogen and progesterone on these ocular structures and on the conjunctiva are contradictory and the mechanisms of action of these female-specific sex hormones in the eye are not well understood. The uncertainty of the effects of oestrogen and progesterone on dry eye symptoms is reflected in the controversial relationship between hormone replacement therapy and the signs and symptoms of dry eye. Current understanding of sex hormone influences on the immune system suggests that oestrogen may modulate a cascade of inflammatory events, which underlie dry eye.

  7. Rapid steroid hormone actions via membrane receptors.

    Science.gov (United States)

    Schwartz, Nofrat; Verma, Anjali; Bivens, Caroline B; Schwartz, Zvi; Boyan, Barbara D

    2016-09-01

    Steroid hormones regulate a wide variety of physiological and developmental functions. Traditional steroid hormone signaling acts through nuclear and cytosolic receptors, altering gene transcription and subsequently regulating cellular activity. This is particularly important in hormonally-responsive cancers, where therapies that target classical steroid hormone receptors have become clinical staples in the treatment and management of disease. Much progress has been made in the last decade in detecting novel receptors and elucidating their mechanisms, particularly their rapid signaling effects and subsequent impact on tumorigenesis. Many of these receptors are membrane-bound and lack DNA-binding sites, functionally separating them from their classical cytosolic receptor counterparts. Membrane-bound receptors have been implicated in a number of pathways that disrupt the cell cycle and impact tumorigenesis. Among these are pathways that involve phospholipase D, phospholipase C, and phosphoinositide-3 kinase. The crosstalk between these pathways has been shown to affect apoptosis and proliferation in cardiac cells, osteoblasts, and chondrocytes as well as cancer cells. This review focuses on rapid signaling by 17β-estradiol and 1α,25-dihydroxy vitamin D3 to examine the integrated actions of classical and rapid steroid signaling pathways both in contrast to each other and in concert with other rapid signaling pathways. This new approach lends insight into rapid signaling by steroid hormones and its potential for use in targeted drug therapies that maximize the benefits of traditional steroid hormone-directed therapies while mitigating their less desirable effects. PMID:27288742

  8. Thyroid Hormones and Growth in Health and Disease

    OpenAIRE

    Tarım, Ömer

    2011-01-01

    Thyroid hormones regulate growth by several mechanisms. In addition to their negative feedback effect on the stimulatory hormones thyrotropin-releasing hormone (TRH) and thyrotropin (TSH), thyroid hormones also regulate their receptors in various physiological and pathological conditions. Up-regulation and down-regulation of the thyroid receptors fine-tune the biological effects exerted by the thyroid hormones. Interestingly, the deiodinase enzyme system is another intrinsic regulator of thyr...

  9. Unraveling the paradoxes of plant hormone signaling integration

    OpenAIRE

    Jaillais, Yvon; Chory, Joanne

    2010-01-01

    Plant hormones play a major role in plant growth and development. They affect similar processes but, paradoxically, their signaling pathways act nonredundantly. Hormone signals are integrated at the gene-network level rather than by cross-talk during signal transduction. In contrast to hormone-hormone integration, recent data suggest that light and plant hormone pathways share common signaling components, which allows photoreceptors to influence the growth program. We propose a role for the p...

  10. Interactions between nitric oxide and plant hormones in aluminum tolerance

    OpenAIRE

    He, Huyi; He, Longfei; Gu, Minghua

    2012-01-01

    Nitric oxide (NO) is involved, together with plant hormones, in the adaptation to Al stress in plants. However, the mechanism by which NO and plant hormones interplay to improve Al tolerance are still unclear. We have recently shown that patterns of plant hormones alteration differ between rye and wheat under Al stress. NO may enhance Al tolerance by regulating hormonal equilibrium in plants, as a regulator of plant hormones signaling. In this paper, some unsolved issues are discussed based o...

  11. Plant hormone signaling during development: insights from computational models

    OpenAIRE

    Oliva, Marina; Farcot, Etienne; Vernoux, Teva

    2013-01-01

    International audience Recent years have seen an impressive increase in our knowledge of the topology of plant hormone signaling networks. The complexity of these topologies has motivated the development of models for several hormones to aid understanding of how signaling networks process hormonal inputs. Such work has generated essential insights into the mechanisms of hormone perception and of regulation of cellular responses such as transcription in response to hormones. In addition, mo...

  12. Efficacy and Safety of Sustained-Release Recombinant Human Growth Hormone in Korean Adults with Growth Hormone Deficiency

    OpenAIRE

    Kim, Youngsook; Hong, Jae Won; Chung, Yoon-Sok; Kim, Sung-Woon; Cho, Yong-Wook; Kim, Jin Hwa; Kim, Byung-Joon; Lee, Eun Jig

    2014-01-01

    Purpose The administration of recombinant human growth hormone in adults with growth hormone deficiency has been known to improve metabolic impairment and quality of life. Patients, however, have to tolerate daily injections of growth hormone. The efficacy, safety, and compliance of weekly administered sustained-release recombinant human growth hormone (SR-rhGH, Declage™) supplement in patients with growth hormone deficiency were evaluated. Materials and Methods This trial is 12-week prospect...

  13. Effectiveness of Low Dose of Gonadotropin Releasing Hormone Agonist on Hormonal Flare-Up

    OpenAIRE

    Bständig, Bettina; Cédrin-Durnerin, Isabelle; Hugues, Jean Noël

    2000-01-01

    Purpose: The hormonal response (flare-up) followingadministration of a standard dose (100 μg) or a low dose(25 μg) of gonadotropin releasing hormone agonist(GnRH-a) (Triptorelin) was compared in patients prior to an in vitrofertilization (IVF) cycle and during the early follicular phaseof a short-term IVF protocol.

  14. Growth Hormone Research Society perspective on the development of long-acting growth hormone preparations

    Science.gov (United States)

    The Growth Hormone (GH) Research Society (GRS) convened a workshop to address important issues regarding trial design, efficacy, and safety of long-acting growth hormone preparations (LAGH). A closed meeting of 55 international scientists with expertise in GH, including pediatric and adult endocrino...

  15. Hormone-dependent aggression in male and female rats: experiential, hormonal, and neural foundations.

    Science.gov (United States)

    Albert, D J; Jonik, R H; Walsh, M L

    1992-01-01

    Hormone-dependent aggression in both male and female rats includes the distinctive behavioral characteristics of piloerection and lateral attack. In males the aggression is dependent on testicular testosterone and is commonly known as intermale aggression. In females, the aggression is most commonly observed as maternal aggression and is dependent on hormones whose identity is only beginning to emerge. The present review examines the experiential events which activate hormone-dependent aggression, the relation of the aggression to gonadal hormones, and the neural structures that participate in its modulation. In males and females, the aggression is activated by cohabitation with a conspecific of the opposite sex, by competitive experience, and by repeated exposure to unfamiliar conspecifics. In the female, the presence of pups also activates aggression. In both males and females, hormones are necessary for the full manifestation of the aggression. The essential hormone appears to be testosterone in males and a combination of testosterone and estradiol in females. The information available suggests the neural control systems for hormone-dependent aggression may be similar in males and females. It is argued that hormone-dependent aggression is behaviorally and biologically homologous in male and female rats.

  16. Single dose and pulsatile treatment with human growth hormone in growth hormone deficiency.

    OpenAIRE

    Smith, P J; Pringle, P J; Brook, C G

    1987-01-01

    The growth and growth hormone profiles in four children receiving three different regimens of treatment with human growth hormone (hGH) were compared. There was no significant difference in the rate of growth between the regimens; the rate of growth fell dramatically after treatment. Pulsatile administration of hGH was no better than conventional treatment.

  17. Thyroid Hormone Receptor alpha Modulates Lipopolysaccharide-Induced Changes in Peripheral Thyroid Hormone Metabolism

    NARCIS (Netherlands)

    J. Kwakkel; O. Chassande; H.C. van Beeren; E. Fliers; W.M. Wiersinga; A. Boelen

    2010-01-01

    Acute inflammation is characterized by low serum T-3 and T-4 levels accompanied by changes in liver type 1 deiodinase (D1), liver D3, muscle D2, and muscle D3 expression. It is unknown at present whether thyroid hormone receptor alpha (TR alpha) plays a role in altered peripheral thyroid hormone met

  18. Sexual hormones in human skin.

    Science.gov (United States)

    Zouboulis, C C; Chen, W-C; Thornton, M J; Qin, K; Rosenfield, R

    2007-02-01

    The skin locally synthesizes significant amounts of sexual hormones with intracrine or paracrine actions. The local level of each sexual steroid depends upon the expression of each of the androgen- and estrogen-synthesizing enzymes in each cell type, with sebaceous glands and sweat glands being the major contributors. Sebocytes express very little of the key enzyme, cytochrome P450c17, necessary for synthesis of the androgenic prohormones dehydroepiandrosterone and androstenedione, however, these prohormones can be converted by sebocytes and sweat glands, and probably also by dermal papilla cells, into more potent androgens like testosterone and dihydrotestosterone. Five major enzymes are involved in the activation and deactivation of androgens in skin. Androgens affect several functions of human skin, such as sebaceous gland growth and differentiation, hair growth, epidermal barrier homeostasis and wound healing. Their effects are mediated by binding to the nuclear androgen receptor. Changes of isoenzyme and/or androgen receptor levels may have important implications in the development of hyperandrogenism and the associated skin diseases such as acne, seborrhoea, hirsutism and androgenetic alopecia. On the other hand, estrogens have been implicated in skin aging, pigmentation, hair growth, sebum production and skin cancer. Estrogens exert their actions through intracellular receptors or via cell surface receptors, which activate specific second messenger signaling pathways. Recent studies suggest specific site-related distribution of ERalpha and ERbeta in human skin. In contrast, progestins play no role in the pathogenesis of skin disorders. However, they play a major role in the treatment of hirsutism and acne vulgaris, where they are prescribed as components of estrogen-progestin combination pills and as anti-androgens. These combinations enhance gonadotropin suppression of ovarian androgen production. Estrogen-progestin treatment can reduce the need for shaving

  19. Sex Hormone Receptor Repertoire in Breast Cancer

    Directory of Open Access Journals (Sweden)

    Gerald M. Higa

    2013-01-01

    Full Text Available Classification of breast cancer as endocrine sensitive, hormone dependent, or estrogen receptor (ER positive refers singularly to ERα. One of the oldest recognized tumor targets, disruption of ERα-mediated signaling, is believed to be the mechanistic mode of action for all hormonal interventions used in treating this disease. Whereas ERα is widely accepted as the single most important predictive factor (for response to endocrine therapy, the presence of the receptor in tumor cells is also of prognostic value. Even though the clinical relevance of the two other sex hormone receptors, namely, ERβ and the androgen receptor remains unclear, two discordant phenomena observed in hormone-dependent breast cancers could be causally related to ERβ-mediated effects and androgenic actions. Nonetheless, our understanding of regulatory molecules and resistance mechanisms remains incomplete, further compromising our ability to develop novel therapeutic strategies that could improve disease outcomes. This review focuses on the receptor-mediated actions of the sex hormones in breast cancer.

  20. How to use and interpret hormone ratios.

    Science.gov (United States)

    Sollberger, Silja; Ehlert, Ulrike

    2016-01-01

    Hormone ratios have become increasingly popular throughout the neuroendocrine literature since they offer a straightforward way to simultaneously analyze the effects of two interdependent hormones. However, the analysis of ratios is associated with statistical and interpretational concerns which have not been sufficiently considered in the context of endocrine research. The aim of this article, therefore, is to demonstrate and discuss these issues, and to suggest suitable ways to address them. In a first step, we use exemplary testosterone and cortisol data to illustrate that one major concern of ratios lies in their distribution and inherent asymmetry. As a consequence, results of parametric statistical analyses are affected by the ultimately arbitrary decision of which way around the ratio is computed (i.e., A/B or B/A). We suggest the use of non-parametric methods as well as the log-transformation of hormone ratios as appropriate methods to deal with these statistical problems. However, in a second step, we also discuss the complicated interpretation of ratios, and propose moderation analysis as an alternative and oftentimes more insightful approach to ratio analysis. In conclusion, we suggest that researchers carefully consider which statistical approach is best suited to investigate reciprocal hormone effects. With regard to the hormone ratio method, further research is needed to specify what exactly this index reflects on the biological level and in which cases it is a meaningful variable to analyze.

  1. Sex hormones and brain dopamine functions.

    Science.gov (United States)

    Sotomayor-Zarate, Ramon; Cruz, Gonzalo; Renard, Georgina M; Espinosa, Pedro; Ramirez, Victor D

    2014-01-01

    Sex hormones exert differential effects on a variety of sensitive tissues like the reproductive tract, gonads, liver, bone and adipose tissue, among others. In the brain, sex hormones act as neuroactive steroids regulating the function of neuroendocrine diencephalic structures like the hypothalamus. In addition, steroids can exert physiological effects upon cortical, limbic and midbrain structures, influencing different behaviors such as memory, learning, mood and reward. In the last three decades, the role of sex hormones on monoamine neurotransmitters in extra-hypothalamic areas related to motivated behaviors, learning and locomotion has been the focus of much research. The purpose of this thematic issue is to present the state of art concerning the effects of sex hormones on the neurochemical regulation of dopaminergic midbrain areas involved in neurobiological and pathological processes, such as addiction to drugs of abuse. We also discuss evidence of how neonatal exposure to sex hormones or endocrine disrupting chemicals can produce long-term changes on the neurochemical regulation of dopaminergic neurons in the limbic and midbrain areas. PMID:25540983

  2. Role of Hormones and Neurosteroids in Epileptogenesis

    Directory of Open Access Journals (Sweden)

    D Samba eReddy

    2013-07-01

    Full Text Available This article describes the emerging evidence of hormonal influence on epileptogenesis, which is a process whereby a brain becomes progressively epileptic due to an initial precipitating event of diverse origin such as brain injury, stroke, infection, or prolonged seizures. The molecular mechanisms underlying the development of epilepsy are poorly understood. Neuroinflammation and neurodegeneration appear to trigger epileptogenesis. There is an intense search for drugs that truly prevent the development of epilepsy in people at risk. Hormones play an important role in children and adults with epilepsy. Corticosteroids, progesterone, estrogens, and neurosteroids have been shown to affect seizure activity in animal models and in clinical studies. However, the impact of hormones on epileptogenesis has not been investigated widely. There is emerging new evidence that progesterone, neurosteroids, and endogenous hormones may play a role in regulating the epileptogenesis. Corticosterone has excitatory effects and triggers epileptogenesis in animal models. Progesterone has disease-modifying activity in epileptogenic models. The antiepileptogenic effect of progesterone has been attributed to its conversion to neurosteroids, which binds to GABA-A receptors and enhances phasic and tonic inhibition in the brain. Neurosteroids are robust anticonvulsants. There is pilot evidence that neurosteroids may have antiepileptogenic properties. Future studies may generate new insight on the disease-modifying potential of hormonal agents and neurosteroids in epileptogenesis.

  3. Hormonal control of sulfate uptake and assimilation.

    Science.gov (United States)

    Koprivova, Anna; Kopriva, Stanislav

    2016-08-01

    Plant hormones have a plethora of functions in control of plant development, stress response, and primary metabolism, including nutrient homeostasis. In the plant nutrition, the interplay of hormones with responses to nitrate and phosphate deficiency is well described, but relatively little is known about the interaction between phytohormones and regulation of sulfur metabolism. As for other nutrients, sulfate deficiency results in modulation of root architecture, where hormones are expected to play an important role. Accordingly, sulfate deficiency induces genes involved in metabolism of tryptophane and auxin. Also jasmonate biosynthesis is induced, pointing to the need of increase the defense capabilities of the plants when sulfur is limiting. However, hormones affect also sulfate uptake and assimilation. The pathway is coordinately induced by jasmonate and the key enzyme, adenosine 5'-phosphosulfate reductase, is additionally regulated by ethylene, abscisic acid, nitric oxid, and other phytohormones. Perhaps the most intriguing link between hormones and sulfate assimilation is the fact that the main regulator of the response to sulfate starvation, SULFATE LIMITATION1 (SLIM1) belongs to the family of ethylene related transcription factors. We will review the current knowledge of interplay between phytohormones and control of sulfur metabolism and discuss the main open questions. PMID:26810064

  4. THE SIGNIFICANCE OF STRESS HORMONES IN PSORIASIS

    Directory of Open Access Journals (Sweden)

    F Z Zangeneh

    2008-12-01

    Full Text Available "nPsoriasis is a chronic, non-contagious skin condition characterized by inflamed and scaly lesions of skin. Whilst the pathogenesis of psoriasis is not known, psychological stress has been implicated as a potential trigger in the onset and exacerbation of the condition. Psychiatric and psychological factors play an important role in at least 30% of dermatologic disorder and pathophysiologic link between psychological stress (PS and disease expression remains unclear. Recent studies demonstrated PS-induced alterations in permeability barrier homeostasis, mediated by increased endogenous glucocorticoids. As activation of the hypothalamic pituitary adrenal axis (HPA is critical to a successful stress response, we investigated this in patients with psoriasis. This study was performed on 55 patients (40 females and 15 males visited our clinic for treatment of psoriasis in pharmacology department. We measured the rate of activation of HPA by hormonal changes. These patients displayed higher fasting blood sugar (FBS, epinephrine (Ep, adrenocorticotropin hormone (ACTH, aldosterone, prolactin, growth hormone and estradiol hormones value but diminished cortisol and corticotropin releasing factor (CRF. These results show that HPA and psychoneuroendocrine hormones have a significant role in psoriasis.

  5. Estrogen and Growth Hormone and their Roles in Reproductive Function

    Directory of Open Access Journals (Sweden)

    Hüseyin Baki ÇİFTCİ

    2013-02-01

    Full Text Available The aim of this study was to review the effect of estrogen on growth hormone secretion and the roles of estrogen and growth hormone in reproductive function. Estrogen is the main hormone affecting growth, development, maturation and functioning of reproductive tract as well as the sexual differentiation and the behavior. Growth hormone is also important factor in sexual maturation and attainment of puberty. The impact of estrogen on growth hormone secretion has been reported in rodents and primates. However, the precise mechanism for the alterations in growth hormone secretion is not clearly known. Estrogen may possibility have a direct affect on growth hormone secretion via the binding to estrogen receptor-α due to its co-expression in growth hormone neurons in the medial preoptic area and arcuate nucleus. Estrogen may also have an indirect effect via the reducing insulin-like growth factor-1 feedback inhibition resulting with increased growth hormone secretion.

  6. Exogenous growth hormone inhibits growth hormone-releasing factor-induced growth hormone secretion in normal men.

    OpenAIRE

    Rosenthal, S M; Hulse, J A; Kaplan, S L; Grumbach, M. M.

    1986-01-01

    Previous studies from this laboratory and by others in rats, monkeys, and humans support the concept that growth hormone (GH) can regulate its own secretion through an autofeedback mechanism. With the availability of human growth hormone-releasing factor (GRF), the possible existence of such a mechanism was reexplored by examining the effect of exogenous GH on the GH response induced by GRF-44-NH2 in six normal men (mean age, 32.4 yr). In all subjects the plasma GH response evoked by GRF-44-N...

  7. Longitudinal reproductive hormone profiles in infants

    DEFF Research Database (Denmark)

    Andersson, A M; Toppari, J; Haavisto, A M;

    1998-01-01

    The gonads are usually considered quiescent organs in infancy and childhood. However, during the first few postnatal months of life, levels of gonadotropins and sex hormones are elevated in humans. Recent epidemiological evidence suggests that environmental factors operating perinatally may...... influence male reproductive health in adulthood. The early postnatal activity of the Sertoli cell, a testicular cell type that is supposed to play a major role in sperm production in adulthood is largely unknown. Recently, the peptide hormone inhibin B was shown to be a marker of Sertoli cell function......, and testosterone. Thus, although levels of FSH, LH, and testosterone decreased into the range observed later in childhood by the age of 6-9 months, serum inhibin B levels remained elevated up to at least the age of 15 months. In girls, the hormonal pattern was generally more complex, with a high interindividual...

  8. Nuclear translocation and retention of growth hormone

    DEFF Research Database (Denmark)

    Mertani, Hichem C; Raccurt, Mireille; Abbate, Aude;

    2003-01-01

    We have previously demonstrated that GH is subject to rapid receptor-dependent nuclear translocation. Here, we examine the importance of ligand activation of the GH-receptor (GHR)-associated Janus kinase (JAK) 2 and receptor dimerization for hormone internalization and nuclear translocation by use...... of cells stably transfected with cDNA for the GHR. Staurosporine and herbimycin A treatment of cells did not affect the ability of GH to internalize but resulted in increased nuclear accumulation of hormone. Similarly, receptor mutations, which prevent the association and activation of JAK2, did not affect...... the ability of the hormone to internalize or translocate to the nucleus but resulted in increased nuclear accumulation of GH. These results were observed both by nuclear isolation and confocal laser scanning microscopy. Staurosporine treatment of cells in which human GH (hGH) was targeted to the cytoplasm...

  9. Thyroid Hormones, Oxidative Stress, and Inflammation

    Directory of Open Access Journals (Sweden)

    Antonio Mancini

    2016-01-01

    Full Text Available Inflammation and oxidative stress (OS are closely related processes, as well exemplified in obesity and cardiovascular diseases. OS is also related to hormonal derangement in a reciprocal way. Among the various hormonal influences that operate on the antioxidant balance, thyroid hormones play particularly important roles, since both hyperthyroidism and hypothyroidism have been shown to be associated with OS in animals and humans. In this context, the nonthyroidal illness syndrome (NTIS that typically manifests as reduced conversion of thyroxine (T4 to triiodothyronine (T3 in different acute and chronic systemic conditions is still a debated topic. The pathophysiological mechanisms of this syndrome are reviewed, together with the roles of deiodinases, the enzymes responsible for the conversion of T4 to T3, in both physiological and pathological situations. The presence of OS indexes in NTIS supports the hypothesis that it represents a condition of hypothyroidism at the tissue level and not only an adaptive mechanism to diseases.

  10. Thyroid Hormones, Oxidative Stress, and Inflammation.

    Science.gov (United States)

    Mancini, Antonio; Di Segni, Chantal; Raimondo, Sebastiano; Olivieri, Giulio; Silvestrini, Andrea; Meucci, Elisabetta; Currò, Diego

    2016-01-01

    Inflammation and oxidative stress (OS) are closely related processes, as well exemplified in obesity and cardiovascular diseases. OS is also related to hormonal derangement in a reciprocal way. Among the various hormonal influences that operate on the antioxidant balance, thyroid hormones play particularly important roles, since both hyperthyroidism and hypothyroidism have been shown to be associated with OS in animals and humans. In this context, the nonthyroidal illness syndrome (NTIS) that typically manifests as reduced conversion of thyroxine (T4) to triiodothyronine (T3) in different acute and chronic systemic conditions is still a debated topic. The pathophysiological mechanisms of this syndrome are reviewed, together with the roles of deiodinases, the enzymes responsible for the conversion of T4 to T3, in both physiological and pathological situations. The presence of OS indexes in NTIS supports the hypothesis that it represents a condition of hypothyroidism at the tissue level and not only an adaptive mechanism to diseases.

  11. Should dermatologists prescribe hormonal contraceptives for acne?

    Science.gov (United States)

    Harper, Julie C

    2009-01-01

    ABSTRACT One of the primary factors contributing to the development of acne vulgaris is excess sebum. Sebaceous glands and sebum excretion are regulated, at least in part, by androgen hormones. Acne treatments that block this androgen effect include spironolactone and combination oral contraceptives (COC). Three COC are now FDA approved to treat moderate acne. Dermatologists must become experts at prescribing these hormonal contraceptives. Likewise, it is vital to be aware of contraindications to hormonal contraceptive therapy. Proper patient selection relies on an appropriate medical history and an assessment of blood pressure. A pelvic exam and/or Papanicolaou smear are not required prior to initiating therapy with a COC. It is important to counsel patients about potential adverse effects of COC pills and to establish appropriate expectations concerning acne improvement. PMID:19845722

  12. Thyroid hormone metabolism and environmental chemical exposure

    Directory of Open Access Journals (Sweden)

    Leijs Marike M

    2012-06-01

    Full Text Available Abstract Background Polychlorinated dioxins and –furans (PCDD/Fs and polychlorinated-biphenyls (PCBs are environmental toxicants that have been proven to influence thyroid metabolism both in animal studies and in human beings. In recent years polybrominated diphenyl ethers (PBDEs also have been found to have a negative influence on thyroid hormone metabolism. The lower brominated flame retardants are now banned in the EU, however higher brominated decabromo-diphenyl ether (DBDE and the brominated flame retardant hexabromocyclododecane (HBCD are not yet banned. They too can negatively influence thyroid hormone metabolism. An additional brominated flame retardant that is still in use is tetrabromobisphenol-A (TBBPA, which has also been shown to influence thyroid hormone metabolism. Influences of brominated flame retardants, PCDD/F’s and dioxin like-PCBs (dl-PCB’s on thyroid hormone metabolism in adolescence in the Netherlands will be presented in this study and determined if there are reasons for concern to human health for these toxins. In the period 1987-1991, a cohort of mother-baby pairs was formed in order to detect abnormalities in relation to dioxin levels in the perinatal period. The study demonstrated that PCDD/Fs were found around the time of birth, suggesting a modulation of the setpoint of thyroid hormone metabolism with a higher 3,3’, 5,5’tetrathyroxine (T4 levels and an increased thyroid stimulating hormone (TSH. While the same serum thyroid hormone tests (- TSH and T4 were again normal by 2 years of age and were still normal at 8-12 years, adolescence is a period with extra stress on thyroid hormone metabolism. Therefore we measured serum levels of TSH, T4, 3,3’,5- triiodothyronine (T3, free T4 (FT4, antibodies and thyroxine-binding globulin (TBG in our adolescent cohort. Methods Vena puncture was performed to obtain samples for the measurement of thyroid hormone metabolism related parameters and the current serum dioxin

  13. Gravitational effects on plant growth hormone concentration

    Science.gov (United States)

    Bandurski, R. S.; Schulze, A.

    1983-01-01

    Dolk's (1936) finding that more growth hormone diffuses from the lower side of a gravity-stimulated plant shoot than from the upper side is presently confirmed by means of both an isotope dilution assay and selected ion monitoring-gas chromatography-mass spectrometry, and it is established that the asymmetrically distributed hormone is indole-3-acetic acid (IAA). This is the first physicochemical demonstration that there is more IAA on the lower sides of a geostimulated plant shoot. It is also found that free IAA primarily occurs in the conductive vascular tissues of the shoot, while IAA esters predominate in the growing cortical cells. A highly sensitive gas chromatographic isotope dilution assay shows that the hormone asymmetry also occurs in the nonvascular tissue.

  14. Plant hormone interactions: how complex are they?

    Science.gov (United States)

    Ross, John J; Weston, Diana E; Davidson, Sandra E; Reid, James B

    2011-04-01

    Models describing plant hormone interactions are often complex and web-like. Here we assess several suggested interactions within one experimental system, elongating pea internodes. Results from this system indicate that at least some suggested interactions between auxin, gibberellins (GAs), brassinosteroids (BRs), abscisic acid (ABA) and ethylene do not occur in this system or occur in the reverse direction to that suggested. Furthermore, some of the interactions are relatively weak and may be of little physiological relevance. This is especially true if plant hormones are assumed to show a log-linear response curve as many empirical results suggest. Although there is strong evidence to support some interactions between hormones (e.g. auxin stimulating ethylene and bioactive GA levels), at least some of the web-like complexities do not appear to be justified or are overstated. Simpler and more targeted models may be developed by dissecting out key interactions with major physiological effects. PMID:21214880

  15. Hormonal replacement therapy and gynecological cancer.

    Science.gov (United States)

    Onnis, A; Marchetti, M

    1999-01-01

    The problem of quality of life and lifestyle in elderly women is today a very important social problem all over the world but particularly in rich western countries. Life expectancy of the population will be longer and longer in the future and for both females and males the biological involution correlated with the aging process must be delayed. The gonadal hormones stimulate the healthy state of the entire body (heart, skin, brain, bones, urogenital apparatus and so on) and consequently hormonal replacement therapy (HRT) is mandatory. In women the biological clock of menopause allows us to intervene at the right time, with personalized estrogenic, estroprogestinic or estroandrogenic treatments. Health benefits and groundless risks allow today a careful hormonal management even in women treated for gynaecological cancers (breast and endometrium as well). PMID:10412612

  16. Effects of anti - müllerian hormone and inhibin B on the outcome of in vitro fertili-zation - embryo transfer%抗苗勒激素与抑制素B对体外受精-胚胎移植结局的影响

    Institute of Scientific and Technical Information of China (English)

    程萍; 张昌军; 王华

    2012-01-01

    Objective; To research the relationship between anti -mullerian hormone (AMH) and inhibin B in serum and follicu-lar fluid and the development of follicles, ovarian reaction ability, quality of embryos, and pregnancy outcome of the patients receiving in vitro fertilization - embryo transfer (IVF - ET) . Methods; Enzyme - linked immunosorbent assay ( ELISA) was used to detect the levels of AMH and inhibin B in serum during the early follicular phase (on the third day of menstruation) , middle follicular phase, on the day of oocyte pick - up (OPU), on the day of embryo transfer, on the third day after embryo transfer and the levels of AMH and inhibin B in follicular fluid on the day of OPU; the relationship between the levels of AMH and inhibin B and follicular development, ovarian reaction ability, quality of embryo, and pregnancy outcome was analyzed. Results; The serum level of inhibin B increased gradually with the development of follicles, while the level of serum AMH decreased gradually (P 0. 05), the level of AMH in follicular fluid in pregnancy group was significantly higher than that in non - pregnancy group (P <0. 05) . Conclusion; The serum levels of inhibin B at different phases of COH only can reflect ovarian reaction ability, and they cant predict the pregnancy outcome of IVF - ET; there is no correlation between the level of inhibin B in follicular fluid on the day of OPU andpregnancy outcome. The serum levels of AMH at different phases of COH cant predict the pregnancy outcome of IVF - ET, the level ofAMH in follicular fluid on the day of OPU can be used as a predictive factor of pregnancy outcome of IVF - ET. AMH in follicular fluid can induce the change of embryonic quality by changing the focal microenvironment, then it can affect the outcome of IVF - ET.%目的:研究行体外受精-胚胎移植( IVF - ET)的患者血清及卵泡液中抗苗勒激素(AMH)、抑制素B(IN-HB)与卵泡发育、卵巢反应能力、胚胎质量及妊娠结局之间

  17. Hemostatic Disorders in Hormonally Active Pituitary Tumors.

    Science.gov (United States)

    Świątkowska-Stodulska, R; Babińska, A; Mital, A; Stodulski, D; Sworczak, K

    2015-10-01

    Endocrinopathies encompass heterogeneous diseases that can lead to hemostasis disorders at various stages over their clinical course. Normal hemostasis requires an equilibrium between the processes of coagulation and fibrinolysis, which depend on multiple activators and inhibitors. To date, the influence of various hormonal disorders on the hemostatic system has been assessed many times. The aim of this review was to analyze hemostasis abnormalities that occur in patients with hormonally active pituitary tumors: corticotropinoma, somatotropinoma, prolactinoma, gonadotropinoma and thyrotropinoma. Authors discuss studies that examined coagulation and hemostasis parameters among patients with these tumors, as well as analyze antithrombotic prophylaxis approach for endogenous hypercortisolemia subjects in particular. PMID:26285071

  18. Parathyroid hormone secretion in chronic renal failure

    DEFF Research Database (Denmark)

    Madsen, J C; Rasmussen, A Q; Ladefoged, S D;

    1996-01-01

    The aim of study was to introduce and evaluate a method for quantifying the parathyroid hormone (PTH) secretion during hemodialysis in secondary hyperparathyroidism due to end-stage renal failure. We developed a method suitable for inducing sequential hypocalcemia and hypercalcemia during....../ionized calcium curves were constructed, and a mean calcium set-point of 1.16 mmol/liter was estimated compared to the normal mean of about 1.13 mmol/liter. In conclusion, we demonstrate that it is important to use a standardized method to evaluate parathyroid hormone dynamics in chronic renal failure. By the use...

  19. Antiandrogen and hormonal treatment of acne.

    Science.gov (United States)

    Shaw, J C

    1996-10-01

    In the treatment of acne in women, the use of antiandrogens and other hormonal approaches is a valuable alternative to standard therapy. These treatments that are based on physiologically sound principles produce gratifying results in selected women with acne, and are the primary treatment for women with hirsutism. The drugs discussed in this article include spironolactone, cyproterone acetate, flutamide, oral contraceptives, corticosteroids, finasteride, and gonadotropin-releasing hormone agonists. Patient selection, pretreatment evaluation, and case studies also are discussed with an emphasis on practical applications. PMID:9238337

  20. How sex hormones promote skeletal muscle regeneration.

    Science.gov (United States)

    Velders, Martina; Diel, Patrick

    2013-11-01

    Skeletal muscle regeneration efficiency declines with age for both men and women. This decline impacts on functional capabilities in the elderly and limits their ability to engage in regular physical activity and to maintain independence. Aging is associated with a decline in sex hormone production. Therefore, elucidating the effects of sex hormone substitution on skeletal muscle homeostasis and regeneration after injury or disuse is highly relevant for the aging population, where sarcopenia affects more than 30 % of individuals over 60 years of age. While the anabolic effects of androgens are well known, the effects of estrogens on skeletal muscle anabolism have only been uncovered in recent times. Hence, the purpose of this review is to provide a mechanistic insight into the regulation of skeletal muscle regenerative processes by both androgens and estrogens. Animal studies using estrogen receptor (ER) antagonists and receptor subtype selective agonists have revealed that estrogens act through both genomic and non-genomic pathways to reduce leukocyte invasion and increase satellite cell numbers in regenerating skeletal muscle tissue. Although animal studies have been more conclusive than human studies in establishing a role for sex hormones in the attenuation of muscle damage, data from a number of recent well controlled human studies is presented to support the notion that hormonal therapies and exercise induce added positive effects on functional measures and lean tissue mass. Based on the fact that aging human skeletal muscle retains the ability to adapt to exercise with enhanced satellite cell activation, combining sex hormone therapies with exercise may induce additive effects on satellite cell accretion. There is evidence to suggest that there is a 'window of opportunity' after the onset of a hypogonadal state such as menopause, to initiate a hormonal therapy in order to achieve maximal benefits for skeletal muscle health. Novel receptor subtype selective

  1. Incretin hormones and the satiation signal

    DEFF Research Database (Denmark)

    Holst, Jens Juul

    2013-01-01

    Recent research has indicated that appetite-regulating hormones from the gut may have therapeutic potential. The incretin hormone, glucagon-like peptide-1 (GLP-1), appears to be involved in both peripheral and central pathways mediating satiation. Several studies have also indicated that GLP-1 le...... subjects without diabetes. Thus, GLP-1 RAs may provide a new option for pharmacological treatment of obesity....... levels and responses to meals may be altered in obese subjects. Clinical trial results have shown further that two GLP-1 receptor agonists (GLP-1 RAs), exenatide and liraglutide, which are approved for the treatment of hyperglycemia in patients with type 2 diabetes, also produce weight loss in overweight...

  2. Sex hormone replacement in Turner syndrome

    DEFF Research Database (Denmark)

    Trolle, Christian; Hjerrild, Britta; Cleemann, Line Hartvig;

    2012-01-01

    The cardinal features of Turner syndrome (TS) are short stature, congenital abnormalities, infertility due to gonadal dysgenesis, with sex hormone insufficiency ensuing from premature ovarian failure, which is involved in lack of proper development of secondary sex characteristics and the frequent...... osteoporosis seen in Turner syndrome. But sex hormone insufficiency is also involved in the increased cardiovascular risk, state of physical fitness, insulin resistance, body composition, and may play a role in the increased incidence of autoimmunity. Severe morbidity and mortality affects females with Turner...

  3. Oral manifestations in growth hormone disorders

    Directory of Open Access Journals (Sweden)

    Gaurav Atreja

    2012-01-01

    Full Text Available Growth hormone is of vital importance for normal growth and development. Individuals with growth hormone deficiency develop pituitary dwarfism with disproportionate delayed growth of skull and facial skeleton giving them a small facial appearance for their age. Both hyper and hypopituitarism have a marked effect on development of oro-facial structures including eruption and shedding patterns of teeth, thus giving an opportunity to treating dental professionals to first see the signs and symptoms of these growth disorders and correctly diagnose the serious underlying disease.

  4. Clinical Trials in Male Hormonal Contraception

    Directory of Open Access Journals (Sweden)

    Nieschlag E

    2011-01-01

    Full Text Available Research has established the principle of hormonal male contraception based on suppression of gonadotropins and spermatogenesis. All hormonal male contraceptives use testosterone, but only in East Asian men can testosterone alone suppress spermatogenesis to a level compatible with contraceptive protection. In Caucasians, additional agents are required of which progestins are favored. Clinical trials concentrate on testosterone combined with norethisterone, desogestrel, etonogestrel or depot-medroxyprogesterone acetate. The first randomized, placebo-controlled clinical trial performed by the pharmaceutical industry demonstrated the effectiveness of a combination of testosterone undecanoate and etonogestrel in suppressing spermatogenesis in volunteers.

  5. How sex hormones promote skeletal muscle regeneration.

    Science.gov (United States)

    Velders, Martina; Diel, Patrick

    2013-11-01

    Skeletal muscle regeneration efficiency declines with age for both men and women. This decline impacts on functional capabilities in the elderly and limits their ability to engage in regular physical activity and to maintain independence. Aging is associated with a decline in sex hormone production. Therefore, elucidating the effects of sex hormone substitution on skeletal muscle homeostasis and regeneration after injury or disuse is highly relevant for the aging population, where sarcopenia affects more than 30 % of individuals over 60 years of age. While the anabolic effects of androgens are well known, the effects of estrogens on skeletal muscle anabolism have only been uncovered in recent times. Hence, the purpose of this review is to provide a mechanistic insight into the regulation of skeletal muscle regenerative processes by both androgens and estrogens. Animal studies using estrogen receptor (ER) antagonists and receptor subtype selective agonists have revealed that estrogens act through both genomic and non-genomic pathways to reduce leukocyte invasion and increase satellite cell numbers in regenerating skeletal muscle tissue. Although animal studies have been more conclusive than human studies in establishing a role for sex hormones in the attenuation of muscle damage, data from a number of recent well controlled human studies is presented to support the notion that hormonal therapies and exercise induce added positive effects on functional measures and lean tissue mass. Based on the fact that aging human skeletal muscle retains the ability to adapt to exercise with enhanced satellite cell activation, combining sex hormone therapies with exercise may induce additive effects on satellite cell accretion. There is evidence to suggest that there is a 'window of opportunity' after the onset of a hypogonadal state such as menopause, to initiate a hormonal therapy in order to achieve maximal benefits for skeletal muscle health. Novel receptor subtype selective

  6. Negative regulation of parathyroid hormone-related protein expression by steroid hormones

    International Nuclear Information System (INIS)

    Highlights: → Steroid hormones repress expression of PTHrP in the cell lines where the corresponding nuclear receptors are expressed. → Nuclear receptors are required for suppression of PTHrP expression by steroid hormones, except for androgen receptor. → Androgen-induced suppression of PTHrP expression appears to be mediated by estrogen receptor. -- Abstract: Elevated parathyroid hormone-related protein (PTHrP) is responsible for humoral hypercalcemia of malignancy (HHM), which is of clinical significance in treatment of terminal patients with malignancies. Steroid hormones were known to cause suppression of PTHrP expression. However, detailed studies linking multiple steroid hormones to PTHrP expression are lacking. Here we studied PTHrP expression in response to steroid hormones in four cell lines with excessive PTHrP production. Our study established that steroid hormones negatively regulate PTHrP expression. Vitamin D receptor, estrogen receptor α, glucocorticoid receptor, and progesterone receptor, were required for repression of PTHrP expression by the cognate ligands. A notable exception was the androgen receptor, which was dispensable for suppression of PTHrP expression in androgen-treated cells. We propose a pathway(s) involving nuclear receptors to suppress PTHrP expression.

  7. Sex, hormones and neurogenesis in the hippocampus: hormonal modulation of neurogenesis and potential functional implications.

    Science.gov (United States)

    Galea, L A M; Wainwright, S R; Roes, M M; Duarte-Guterman, P; Chow, C; Hamson, D K

    2013-11-01

    The hippocampus is an area of the brain that undergoes dramatic plasticity in response to experience and hormone exposure. The hippocampus retains the ability to produce new neurones in most mammalian species and is a structure that is targeted in a number of neurodegenerative and neuropsychiatric diseases, many of which are influenced by both sex and sex hormone exposure. Intriguingly, gonadal and adrenal hormones affect the structure and function of the hippocampus differently in males and females. Adult neurogenesis in the hippocampus is regulated by both gonadal and adrenal hormones in a sex- and experience-dependent way. Sex differences in the effects of steroid hormones to modulate hippocampal plasticity should not be completely unexpected because the physiology of males and females is different, with the most notable difference being that females gestate and nurse the offspring. Furthermore, reproductive experience (i.e. pregnancy and mothering) results in permanent changes to the maternal brain, including the hippocampus. This review outlines the ability of gonadal and stress hormones to modulate multiple aspects of neurogenesis (cell proliferation and cell survival) in both male and female rodents. The function of adult neurogenesis in the hippocampus is linked to spatial memory and depression, and the present review provides early evidence of the functional links between the hormonal modulation of neurogenesis that may contribute to the regulation of cognition and stress.

  8. Negative regulation of parathyroid hormone-related protein expression by steroid hormones

    Energy Technology Data Exchange (ETDEWEB)

    Kajitani, Takashi; Tamamori-Adachi, Mimi [Department of Biochemistry, Teikyo University School of Medicine, 2-11-1 Kaga, Itabashi-ku, Tokyo 173-8605 (Japan); Okinaga, Hiroko [Department of Internal Medicine, Teikyo University School of Medicine, 2-11-1 Kaga, Itabashi-ku, Tokyo 173-8605 (Japan); Chikamori, Minoru; Iizuka, Masayoshi [Department of Biochemistry, Teikyo University School of Medicine, 2-11-1 Kaga, Itabashi-ku, Tokyo 173-8605 (Japan); Okazaki, Tomoki, E-mail: okbgeni@med.teikyo-u.ac.jp [Department of Biochemistry, Teikyo University School of Medicine, 2-11-1 Kaga, Itabashi-ku, Tokyo 173-8605 (Japan)

    2011-04-15

    Highlights: {yields} Steroid hormones repress expression of PTHrP in the cell lines where the corresponding nuclear receptors are expressed. {yields} Nuclear receptors are required for suppression of PTHrP expression by steroid hormones, except for androgen receptor. {yields} Androgen-induced suppression of PTHrP expression appears to be mediated by estrogen receptor. -- Abstract: Elevated parathyroid hormone-related protein (PTHrP) is responsible for humoral hypercalcemia of malignancy (HHM), which is of clinical significance in treatment of terminal patients with malignancies. Steroid hormones were known to cause suppression of PTHrP expression. However, detailed studies linking multiple steroid hormones to PTHrP expression are lacking. Here we studied PTHrP expression in response to steroid hormones in four cell lines with excessive PTHrP production. Our study established that steroid hormones negatively regulate PTHrP expression. Vitamin D receptor, estrogen receptor {alpha}, glucocorticoid receptor, and progesterone receptor, were required for repression of PTHrP expression by the cognate ligands. A notable exception was the androgen receptor, which was dispensable for suppression of PTHrP expression in androgen-treated cells. We propose a pathway(s) involving nuclear receptors to suppress PTHrP expression.

  9. Endogenous growth hormone (GH)-releasing hormone is required for GH responses to pharmacological stimuli.

    OpenAIRE

    Jaffe, C A; DeMott-Friberg, R; Barkan, A. L.

    1996-01-01

    The roles of hypothalamic growth hormone-releasing hormone (GHRH) and of somatostatin (SRIF) in pharmacologically stimulated growth hormone (GH) secretion in humans are unclear. GH responses could result either from GHRH release or from acute decline in SRIF secretion. To assess directly the role of endogenous GHRH in human GH secretion, we have used a competitive GHRH antagonist, (N-Ac-Tyr1,D-Arg2)GHRH(1-29)NH2 (GHRH-Ant), which we have previously shown is able to block the GH response to GH...

  10. How Much Does AMH Really Vary in Normal Women?

    Directory of Open Access Journals (Sweden)

    Antonio La Marca

    2013-01-01

    Full Text Available Anti-Mullerian Hormone (AMH is an ovarian hormone expressed in growing follicles that have undergone recruitment from the primordial follicle pool but have not yet been selected for dominance. It is considered an accurate marker of ovarian reserve, able to reflect the size of the ovarian follicular pool of a woman of reproductive age. In comparison to other hormonal biomarkers such as serum FSH, low intra- and intermenstrual cycle variability have been proposed for AMH. This review summarizes the knowledge regarding within-subject variability, with particular attention on AMH intracycle variability. Moreover the impact of ethnicity, body mass index, and smoking behaviour on AMH interindividual variability will be reviewed. Finally changes in AMH serum levels in two conditions of ovarian quiescence, namely contraceptives use and pregnancy, will be discussed. The present review aims at guiding researchers and clinicians in interpreting AMH values and fluctuations in various research and clinical scenarios.

  11. How Much Does AMH Really Vary in Normal Women?

    Science.gov (United States)

    La Marca, Antonio; Grisendi, Valentina; Griesinger, Georg

    2013-01-01

    Anti-Mullerian Hormone (AMH) is an ovarian hormone expressed in growing follicles that have undergone recruitment from the primordial follicle pool but have not yet been selected for dominance. It is considered an accurate marker of ovarian reserve, able to reflect the size of the ovarian follicular pool of a woman of reproductive age. In comparison to other hormonal biomarkers such as serum FSH, low intra- and intermenstrual cycle variability have been proposed for AMH. This review summarizes the knowledge regarding within-subject variability, with particular attention on AMH intracycle variability. Moreover the impact of ethnicity, body mass index, and smoking behaviour on AMH interindividual variability will be reviewed. Finally changes in AMH serum levels in two conditions of ovarian quiescence, namely contraceptives use and pregnancy, will be discussed. The present review aims at guiding researchers and clinicians in interpreting AMH values and fluctuations in various research and clinical scenarios.

  12. Development of Chemiluminscence Immunoaasy Kit for Follicle-Stimulating Hormone

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    <正>Follicle-stimulating hormone (FSH) is a gonadotropic hormone, and it is synthesized and secreted by basophilic cell of anterior lobe of hypophysis. Detection of FSH levels in human serum is useful in

  13. Hormone Therapy Won't Help Memory After Menopause

    Science.gov (United States)

    ... page: https://medlineplus.gov/news/fullstory_159955.html Hormone Therapy Won't Help Memory After Menopause 5- ... important risk cognitively associated with the use of hormone therapy over at least five years," said lead ...

  14. Effects of phenobarbital on thyroid hormone contabolism in rat hepatocytes

    Science.gov (United States)

    Hepatic enzyme inducers such as phenobarbital (PB) decrease circulating thyroid hormone (TH) concentrations in rodents. PB induction of hepatic xenobiotic metabolizing enzymes increases thyroid hormones catabolism and biliary elimination. This study examines the catabolism and cl...

  15. The impact of recombinant parathyroid hormone on malignancies and mortality

    DEFF Research Database (Denmark)

    Bang, U C; Hyldstrup, L; Jensen, J E B

    2014-01-01

    We used Danish registers to identify patients with osteoporosis, who had been treated with parathyroid hormone and evaluated the probability of developing cancer. We did not find an increased risk of cancer among the patients treated with parathyroid hormone....

  16. Interactions between hormonal contraception and antiepileptic drugs

    DEFF Research Database (Denmark)

    Reimers, Arne; Brodtkorb, Eylert; Sabers, Anne

    2015-01-01

    combinations, most of these drug interactions are predictable and, thus, avoidable. Unfortunately, there is a substantial lack of data regarding the newer AEDs. Detailed understanding of these issues is necessary for those who prescribe AEDs and/or hormonal contraception to women with epilepsy, as well...

  17. THYROID HORMONE DISRUPTION: FROM KINETICS TO DYNAMICS.

    Science.gov (United States)

    A wide range of chemicals with diverse structures act as thyroid disrupting chemicals (TDCs). Broadly defined, TDCs are chemicals that alter the structure or function of the thyroid gland, alter regulatory enzymes associated with thyroid hormones (THs), or change circulating or t...

  18. Urinary growth hormone excretion in acromegaly

    DEFF Research Database (Denmark)

    Main, K M; Lindholm, J; Vandeweghe, M;

    1993-01-01

    The biochemical assessment of disease activity in acromegaly still presents a problem, especially in treated patients with mild clinical symptoms. We therefore examined the diagnostic value of the measurement of urinary growth hormone (GH) excretion in seventy unselected patients with acromegaly...

  19. Hormone replacement therapy and risk of glioma

    DEFF Research Database (Denmark)

    Andersen, Lene; Friis, Søren; Hallas, Jesper;

    2013-01-01

    Aim: Several studies indicate that use of hormone replacement therapy (HRT) is associated with an increased risk of intracranial meningioma, while associations between HRT use and risk of other brain tumors have been less explored. We investigated the influence of HRT use on the risk of glioma...

  20. Determination of hormone parathyroid by radioimmunoassay

    International Nuclear Information System (INIS)

    The labelling of bovine parathyroid hormone and its employment for the determination of seric PTH by radioimmunoanalysis is described. The specific activity of 131I PTH is 200-350mCi/mg and the damage 3-5%. The method used for radioimmunoanalysis was that of C.D. Arnaud and coworkers. (author)

  1. Modelling synergistic effects of appetite regulating hormones

    DEFF Research Database (Denmark)

    Schmidt, Julie Berg; Ritz, Christian

    2016-01-01

    We briefly reviewed one definition of dose addition, which is applicable within the framework of generalized linear models. We established how this definition of dose addition corresponds to effect addition in case only two doses per compound are considered for evaluating synergistic effects. The....... The link between definitions was exemplified for an appetite study where two appetite hormones were studied....

  2. Thyroid hormone action in postnatal heart development

    Directory of Open Access Journals (Sweden)

    Ming Li

    2014-11-01

    Full Text Available Thyroid hormone is a critical regulator of cardiac growth and development, both in fetal life and postnatally. Here we review the role of thyroid hormone in postnatal cardiac development, given recent insights into its role in stimulating a burst of cardiomyocyte proliferation in the murine heart in preadolescence; a response required to meet the massive increase in circulatory demand predicated by an almost quadrupling of body weight during a period of about 21 days from birth to adolescence. Importantly, thyroid hormone metabolism is altered by chronic diseases, such as heart failure and ischemic heart disease, as well as in very sick children requiring surgery for congenital heart diseases, which results in low T3 syndrome that impairs cardiovascular function and is associated with a poor prognosis. Therapy with T3 or thyroid hormone analogs has been shown to improve cardiac contractility; however, the mechanism is as yet unknown. Given the postnatal cardiomyocyte mitogenic potential of T3, its ability to enhance cardiac function by promoting cardiomyocyte proliferation warrants further consideration.

  3. Human Growth Hormone: The Latest Ergogenic Aid?

    Science.gov (United States)

    Cowart, Virginia S.

    1988-01-01

    Believing that synthetic human growth hormone (hGH) will lead to athletic prowess and fortune, some parents and young athletes wish to use the drug to enhance sports performance. Should hGH become widely available, its abuse could present many problems, from potential health risks to the ethics of drug-enhanced athletic performance. (JL)

  4. LEARNING HORMONE ACTION MECHANISMS WITH BIOINFORMATICS

    Directory of Open Access Journals (Sweden)

    João Carlos Sousa

    2007-05-01

    Full Text Available The ability to manage the constantly growing information in genetics availableon the internet is becoming crucial in biochemical education and medicalpractice. Therefore, developing students skills in working with bioinformaticstools is a challenge to undergraduate courses in the molecular life sciences.The regulation of gene transcription by hormones and vitamins is a complextopic that influences all body systems. We describe a student centered activityused in a multidisciplinary “Functional Organ System“ course on the EndocrineSystem. By receiving, as teams, a nucleotide sequence of a hormone orvitamin-response element, students navigate through internet databases to findthe gene to which it belongs. Subsequently, student’s search how thecorresponding hormone/vitamin influences the expression of that particulargene and how a dysfunctional interaction might cause disease. This activity,proposed for 4 consecutive years to cohorts of 50-60 students/year enrolled inthe 2nd year our undergraduate medical degree, revealed that 90% of thestudents developed a better understanding of the usefulness of bioinformaticsand that 98% intend to use them in the future. Since hormones and vitaminsregulate genes of all body organ systems, this web-based activity successfullyintegrates the whole body physiology of the medical curriculum and can be ofrelevance to other courses on molecular life sciences.

  5. Plant hormones and ecophysiology of conifers

    Energy Technology Data Exchange (ETDEWEB)

    Davies, W.J.

    1995-07-01

    Over the past 30 years, there have been very substantial fluctuations in the interests of plant scientists in the involvement of plant growth regulators in the control of physiology, growth, and development of plants. In the years following the identification of the five major classes of growth regulators and identification of other groups of compounds of somewhat more restricted interest, an enormous number of papers reported the effects of hormones applied externally to a very wide range of plants. During this period, it became very fashionable to compare effects of hormones with the effects of the environment on developmental and physiological phenomena and to suggest a regulatory role for the hormone(s) in the processes under consideration. Ross et al. (1983) have published a very comprehensive survey of the effects of growth regulators applied externally to conifers, and even 10 years later, it is difficult to improve on what they have done. Nevertheless, in the light of recent changes in our understanding of how growth regulators may work, it is necessary to reexamine this field and ask what we really know about the involvement of growth regulators in the ecophysiology of conifers.

  6. Pituitary and mammary growth hormone in dogs

    NARCIS (Netherlands)

    Bhatti, Sofie Fatima Mareyam

    2006-01-01

    Several pathological (e.g. obesity and chronic hypercortisolism) and non-pathological (e.g. ageing) states in humans are characterized by a reduction in pituitary growth hormone (GH) secretion. Chronic hypercortisolism in humans is also associated with an impaired GH response to various stimuli. Pit

  7. Impact of Growth Hormone on Cystatin C

    Directory of Open Access Journals (Sweden)

    Lisa Sze

    2013-11-01

    Full Text Available Background: Cystatin C (CysC is an alternative marker to creatinine for estimation of the glomerular filtration rate (GFR. Hormones such as thyroid hormones and glucocorticoids are known to have an impact on CysC. In this study, we examined the effect of growth hormone (GH on CysC in patients with acromegaly undergoing transsphenoidal surgery. Methods: Creatinine, CysC, GH and insulin-like growth factor-1 (IGF-1 were determined in 24 patients with acromegaly before and following transsphenoidal surgery. Estimated GFR was calculated using the Chronic Kidney Disease Epidemiology Collaboration formula. Results: In all patients, surgical debulking resulted in decreased clinical disease activity and declining GH/IGF-1 levels. Postoperatively, biochemical cure was documented in 20 out of 24 patients. Creatinine levels (mean ± SEM increased from 72 ± 3 to 80 ± 3 µmol/l (p = 0.0004 and concurrently, estimated GFR decreased from 99 ± 3 to 91 ± 3 ml/min (p = 0.0008. In contrast to creatinine, CysC levels decreased from 0.72 ± 0.02 to 0.68 ± 0.02 mg/l (p = 0.0008. Conclusions: Our study provides strong evidence for discordant effects of GH on creatinine and CysC in patients with acromegaly undergoing transsphenoidal surgery, thus identifying another hormone that influences CysC independent of renal function.

  8. Lymphocyte GH-axis hormones in immunity.

    Science.gov (United States)

    Weigent, Douglas A

    2013-01-01

    The production and utilization of common ligands and their receptors by cells of the immune and neuroendocrine systems constitutes a biochemical information circuit between and within the immune and neuroendocrine systems. The sharing of ligands and receptors allows the immune system to serve as the sixth sense notifying the nervous system of the presence of foreign entities. Within this framework, it is also clear that immune cell functions can be altered by neuroendocrine hormones and that cells of the immune system have the ability to produce neuroendocrine hormones. This review summarizes a part of this knowledge with particular emphasis on growth hormone (GH). The past two decades have uncovered a lot of detail about the actions of GH, acting through its receptor, at the molecular and cellular level and its influence on the immune system. The production and action of immune cell-derived GH is less well developed although its important role in immunity is also slowly emerging. Here we discuss the production of GH, GH-releasing hormone (GHRH) and insulin-like growth factor-1 (IGF-1) and their cognate receptors on cells of the immune system and their influence via endocrine/autocrine/paracrine and intracrine pathways on immune function. The intracellular mechanisms of action of immune cell-derived GH are still largely unexplored, and it is anticipated that further work in this particular area will establish an important role for this source of GH in normal physiology and in pathologic situations. PMID:24177252

  9. Hormonal contraceptive congruency : Implications for relationship jealousy

    NARCIS (Netherlands)

    Cobey, Kelly D.; Roberts, S. Craig; Buunk, Abraham P.

    2013-01-01

    Research shows that women who use hormonal contraceptives (HCs) differ in their mate preferences from women who have regular cycles. It has been proposed that when a partnered woman either begins to use or ceases to use HCs, she may experience changes in her relationship since her preferences become

  10. Hormone Metabolism During Potato Tuber Dormancy

    Science.gov (United States)

    At harvest and for an indeterminate period thereafter potato tubers will not sprout and are physiologically dormant. The length of tuber dormancy is dependent on cultivar and pre- and postharvest environmental conditions. Plant hormones have been shown to be involved in all phases of dormancy prog...

  11. Justified and unjustified use of growth hormone.

    NARCIS (Netherlands)

    A-J. van der Lely (Aart-Jan)

    2004-01-01

    textabstractGrowth hormone (GH) replacement therapy for children and adults with proven GH deficiency due to a pituitary disorder has become an accepted therapy with proven efficacy. GH is increasingly suggested, however, as a potential treatment for frailty, osteoporosis, morbid o

  12. Growth Hormone Deficiency, Brain Development, and Intelligence

    Science.gov (United States)

    Meyer-Bahlburg, Heino F. L.; And Others

    1978-01-01

    Available from: American Medical Association, 535 N. Dearborn Street, Chicago, Illinois 60610. In order to determine what effect, if any, growth hormone (GH) has on human brain development, 29 patients (mean age 11.7 years) with GH deficiency were selected according to the following criteria: no evidence of reversible GH deficiency, onset of…

  13. Prolactin and growth hormone in fish osmoregulation

    Science.gov (United States)

    Sakamoto, T.; McCormick, S.D.

    2006-01-01

    Prolactin is an important regulator of multiple biological functions in vertebrates, and has been viewed as essential to ion uptake as well as reduction in ion and water permeability of osmoregulatory surfaces in freshwater and euryhaline fish. Prolactin-releasing peptide seems to stimulate prolactin expression in the pituitary and peripheral organs during freshwater adaptation. Growth hormone, a member of the same family of hormones as prolactin, promotes acclimation to seawater in several teleost fish, at least in part through the action of insulin-like growth factor I. In branchial epithelia, development and differentiation of the seawater-type chloride cell (and their underlying biochemistry) is regulated by GH, IGF-I, and cortisol, whereas the freshwater-type chloride cell is regulated by prolactin and cortisol. In the epithelia of gastrointestinal tract, prolactin induces cell proliferation during freshwater adaptation, whereas cortisol stimulates both cell proliferation and apoptosis. We propose that control of salinity acclimation in teleosts by prolactin and growth hormone primarily involves regulation of cell proliferation, apoptosis, and differentiation (the latter including upregulation of specific ion transporters), and that there is an important interaction of these hormones with corticosteroids. ?? 2005 Elsevier Inc. All rights reserved.

  14. Plant Hormones: How They Affect Root Formation.

    Science.gov (United States)

    Reinhard, Diana Hereda

    This science study aid, produced by the U.S. Department of Agriculture, includes a series of plant rooting activities for secondary science classes. The material in the pamphlet is written for students and includes background information on plant hormones, a vocabulary list, and five learning activities. Objectives, needed materials, and…

  15. The impact of female sex hormones on competitiveness

    OpenAIRE

    Buser, T.

    2009-01-01

    We use fluctuations of female sex hormones occurring naturally over the menstrual cycle or induced by hormonal contraceptives to determine the importance of sex hormones in explaining gender differences in competitiveness. Participants in a laboratory experiment solve a simple arithmetics task first under a piece rate and then under a competitive tournament scheme. Subjects can then choose which compensation scheme to apply in a third round. We find that sex hormones have a strong effect on w...

  16. Linker histones in hormonal gene regulation.

    Science.gov (United States)

    Vicent, G P; Wright, R H G; Beato, M

    2016-03-01

    In the present review, we summarize advances in our knowledge on the role of the histone H1 family of proteins in breast cancer cells, focusing on their response to progestins. Histone H1 plays a dual role in gene regulation by hormones, both as a structural component of chromatin and as a dynamic modulator of transcription. It contributes to hormonal regulation of the MMTV promoter by stabilizing a homogeneous nucleosome positioning, which reduces basal transcription whereas at the same time promoting progesterone receptor binding and nucleosome remodeling. These combined effects enhance hormone dependent gene transcription, which eventually requires H1 phosphorylation and displacement. Various isoforms of histone H1 have specific functions in differentiated breast cancer cells and compact nucleosomal arrays to different extents in vitro. Genome-wide studies show that histone H1 has a key role in chromatin dynamics of hormone regulated genes. A complex sequence of enzymatic events, including phosphorylation by CDK2, PARylation by PARP1 and the ATP-dependent activity of NURF, are required for H1 displacement and gene de-repression, as a prerequisite for further nucleosome remodeling. Similarly, during hormone-dependent gene repression a dedicated enzymatic mechanism controls H1 deposition at promoters by a complex containing HP1γ, LSD1 and BRG1, the ATPase of the BAF complex. Thus, a broader vision of the histone code should include histone H1, as the linker histone variants actively participate in the regulation of the chromatin structure. How modifications of the core histones tails affect H1 modifications and vice versa is one of the many questions that remains to be addressed to provide a more comprehensive view of the histone cross-talk mechanisms. PMID:26518266

  17. Linker histones in hormonal gene regulation.

    Science.gov (United States)

    Vicent, G P; Wright, R H G; Beato, M

    2016-03-01

    In the present review, we summarize advances in our knowledge on the role of the histone H1 family of proteins in breast cancer cells, focusing on their response to progestins. Histone H1 plays a dual role in gene regulation by hormones, both as a structural component of chromatin and as a dynamic modulator of transcription. It contributes to hormonal regulation of the MMTV promoter by stabilizing a homogeneous nucleosome positioning, which reduces basal transcription whereas at the same time promoting progesterone receptor binding and nucleosome remodeling. These combined effects enhance hormone dependent gene transcription, which eventually requires H1 phosphorylation and displacement. Various isoforms of histone H1 have specific functions in differentiated breast cancer cells and compact nucleosomal arrays to different extents in vitro. Genome-wide studies show that histone H1 has a key role in chromatin dynamics of hormone regulated genes. A complex sequence of enzymatic events, including phosphorylation by CDK2, PARylation by PARP1 and the ATP-dependent activity of NURF, are required for H1 displacement and gene de-repression, as a prerequisite for further nucleosome remodeling. Similarly, during hormone-dependent gene repression a dedicated enzymatic mechanism controls H1 deposition at promoters by a complex containing HP1γ, LSD1 and BRG1, the ATPase of the BAF complex. Thus, a broader vision of the histone code should include histone H1, as the linker histone variants actively participate in the regulation of the chromatin structure. How modifications of the core histones tails affect H1 modifications and vice versa is one of the many questions that remains to be addressed to provide a more comprehensive view of the histone cross-talk mechanisms.

  18. Growth hormone treatment in non-growth hormone-deficient children

    OpenAIRE

    Loche, Sandro; Carta, Luisanna; Ibba, Anastasia; Guzzetti, Chiara

    2014-01-01

    Until 1985 growth hormone (GH) was obtained from pituitary extracts, and was available in limited amounts only to treat severe growth hormone deficiency (GHD). With the availability of unlimited quantities of GH obtained from recombinant DNA technology, researchers started to explore new modalities to treat GHD children, as well as to treat a number of other non-GHD conditions. Although with some differences between different countries, GH treatment is indicated in children with Turner syndro...

  19. One hundred pregnancies after treatment with pulsatile luteinizing-hormone releasing hormone to induce ovulation

    OpenAIRE

    Homburg, R.; Eshel, A.; Armar, NA; Tucker, M.; Mason, PW; Adams, J.; Kilborn, J.; Sutherland, IA; Jacobs, HS

    1989-01-01

    OBJECTIVE--To review treatment with pulsatile luteinising hormone releasing hormone in infertile women who do not ovulate and are resistant to clomiphene after 100 pregnancies achieved with this treatment. DESIGN--Retrospective analysis of 146 courses of treatment over 434 cycles. SETTING--Infertility clinic. PATIENTS--118 Women whose failure to ovulate was due to idiopathic hypogonadotrophic hypogonadism (n = 39), amenorrhoea related to low weight (n = 17), organic pituitary disease (n = 15)...

  20. Decreased hypothalamic growth hormone-releasing hormone content and pituitary responsiveness in hypothyroidism.

    OpenAIRE

    Katakami, H; Downs, T R; Frohman, L A

    1986-01-01

    The effects of thyroidectomy (Tx) and thyroxine replacement (T4Rx) on pituitary growth hormone (GH) secretion and hypothalamic GH-releasing hormone (GRH) concentration were compared to define the mechanism of hypothyroid-associated GH deficiency. Thyroidectomized rats exhibited a complete loss of pulsatile GH secretion with extensive reduction in GRH responsiveness and pituitary GH content. Cultured pituitary cells from Tx rats exhibited reduced GRH sensitivity, maximal GH responsiveness, and...

  1. The FMR1 Gene, Infertility and Reproductive Decision-Making: A Review

    Directory of Open Access Journals (Sweden)

    Lisa M Pastore

    2014-07-01

    Full Text Available The strongest association between FMR1 and the ovary in humans is the increased risk of premature ovarian failure in women who carry the premutation level of CGG repeats (55-199 CGGs. Research on the FMR1 gene has extended to other endpoints of relevance in the OB/GYN setting for women, including infertility and ovarian hormones. After reviewing the nomenclature changes that have occurred in recent years, this article reviews the evidence linking the length of the FMR1 repeat length to fertility and ovarian hormones (follicle stimulating hormone and anti-mullerian hormone as the primary methods to assess ovarian reserve in clinical settings. The literature is inconsistent on the association between the FMR1 trinucleotide repeat length and infertility. Elevated levels of follicle stimulating hormone have been found in women who carry the premutation; however the literature on the relationship between anti-mullerian hormone and the CGG repeat length are too disparate in design to make a summary statement. This article considers the implications of two transgenic mouse models (FXPM 130R and YAC90R for theories on pathogenesis related to ovarian endpoints. Given the current screening/testing recommendations for reproductive age females and the variability of screening protocols in clinics, future research is recommended on pretest and posttest genetic counseling needs. Future research is also needed on ovarian health measurements across a range of CGG repeat lengths in order to interpret FMR1 test results in reproductive age women; the inconsistencies in the literature make it quite challenging to advise women on their risks related to FMR1 repeat length.

  2. 21 CFR 522.1002 - Follicle stimulating hormone.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 6 2010-04-01 2010-04-01 false Follicle stimulating hormone. 522.1002 Section 522....1002 Follicle stimulating hormone. (a)(1) Specifications. Each package contains 2 vials. One vial... hormone. The other vial contains 10 milliliters of aqueous diluent. (2) Sponsor. See 059521 in §...

  3. Growth hormone deficiency in a girl with the Cohen syndrome.

    OpenAIRE

    Massa, G.; Dooms, L.; Vanderschueren-Lodeweyckx, M

    1991-01-01

    A girl with the Cohen syndrome and isolated growth hormone deficiency is described. Treatment with biosynthetic human growth hormone resulted in marked catch up growth to normal stature. It is concluded that growth hormone deficiency should be ruled out in patients with the Cohen syndrome and small stature.

  4. The impact of female sex hormones on competitiveness

    NARCIS (Netherlands)

    T. Buser

    2009-01-01

    We use fluctuations of female sex hormones occurring naturally over the menstrual cycle or induced by hormonal contraceptives to determine the importance of sex hormones in explaining gender differences in competitiveness. Participants in a laboratory experiment solve a simple arithmetics task first

  5. Overnight levels of luteinizing hormone, follicle-stimulating hormone and growth hormone before and during gonadotropin-releasing hormone analogue treatment in short boys born small for gestational age

    NARCIS (Netherlands)

    D.C.M. van der Kaay (Danielle); F.H. de Jong (Frank); S.R. Rose (Susan); R.J.H. Odink (Roelof); W.M. Bakker-Van Waarde (Willie); E.J. Sulkers (Eric); A.C.S. Hokken-Koelega (Anita)

    2009-01-01

    textabstractAims: To evaluate if 3 months of gonadotropin-releasing hormone analogue (GnRHa) treatment results in sufficient suppression of pubertal luteinizing hormone (LH) and follicle-stimulating hormone (FSH) profile patterns in short pubertal small for gestational age (SGA) boys. To compare gro

  6. Effects of growth hormone deficiency and recombinant growth hormone therapy on postprandial gallbladder motility and cholecystokinin release.

    NARCIS (Netherlands)

    Moschetta, A.; Twickler, M.; Rehfeld, J.F.; Ooteghem, N.A. van; Castro Cabezas, M.; Portincasa, P.; Berge-Henegouwen, G.P. van; Erpecum, K.J. van

    2004-01-01

    In addition to cholecystokinin, other hormones have been suggested to be involved in regulation of postprandial gallbladder contraction. We aimed to evaluate effects of growth hormone (GH) on gallbladder contractility and cholecystokinin release. Gallbladder and gastric emptying (by ultrasound) and

  7. Exogenous Hormone Use: Oral Contraceptives, Postmenopausal Hormone Therapy, and Health Outcomes in the Nurses’ Health Study

    Science.gov (United States)

    Grodstein, Francine; Stampfer, Meir J.; Willett, Walter C.; Hu, Frank B.; Manson, JoAnn E.

    2016-01-01

    Objectives. To review the contribution of the Nurses’ Health Study (NHS) to our understanding of the complex relationship between exogenous hormones and health outcomes in women. Methods. We performed a narrative review of the publications of the NHS and NHS II from 1976 to 2016. Results. Oral contraceptive and postmenopausal hormone use were studied in relation to major health outcomes, including cardiovascular disease and cancer. Current or recent oral contraceptive use is associated with a higher risk of cardiovascular disease (mainly among smokers), melanoma, and breast cancer, and a lower risk of colorectal and ovarian cancer. Although hormone therapy is not indicated primarily for chronic disease prevention, findings from the NHS and a recent analysis of the Women’s Health Initiative indicate that younger women who are closer to menopause onset have a more favorable risk–benefit profile than do older women from use of hormone therapy for relief of vasomotor symptoms. Conclusions. With updated information on hormone use, lifestyle factors, and other variables, the NHS and NHS II continue to contribute to our understanding of the complex relationship between exogenous hormones and health outcomes in women. PMID:27459451

  8. Sex hormones affect neurotransmitters and shape the adult female brain during hormonal transition periods

    Directory of Open Access Journals (Sweden)

    Claudia eBarth

    2015-02-01

    Full Text Available Sex hormones have been implicated in neurite outgrowth, synaptogenesis, dendritic branching, myelination and other important mechanisms of neural plasticity. Here we review the evidence from animal experiments and human studies reporting interactions between sex hormones and the dominant neurotransmitters, such as serotonin, dopamine, GABA and glutamate. We provide an overview of accumulating data during physiological and pathological conditions and discuss currently conceptualized theories on how sex hormones potentially trigger neuroplasticity changes through these four neurochemical systems. Many brain regions have been demonstrated to express high densities for estrogen- and progesterone receptors, such as the amygdala, the hypothalamus, and the hippocampus. As the hippocampus is of particular relevance in the context of mediating structural plasticity in the adult brain, we put particular emphasis on what evidence could be gathered thus far that links differences in behavior, neurochemical patterns and hippocampal structure to a changing hormonal environment. Finally, we discuss how physiologically occurring hormonal transition periods in humans can be used to model how changes in sex hormones influence functional connectivity, neurotransmission and brain structure in vivo.

  9. Structural Basis for Antibody Discrimination between Two Hormones That Recognize the Parathyroid Hormone Receptor

    Energy Technology Data Exchange (ETDEWEB)

    McKinstry, William J.; Polekhina, Galina; Diefenbach-Jagger, Hannelore; Ho, Patricia W.M.; Sato, Koh; Onuma, Etsuro; Gillespie, Matthew T.; Martin, T. John; Parker, Michael W.; (SVIMR-A); (Chugai); (Melbourne)

    2009-08-18

    Parathyroid hormone-related protein (PTHrP) plays a vital role in the embryonic development of the skeleton and other tissues. When it is produced in excess by cancers it can cause hypercalcemia, and its local production by breast cancer cells has been implicated in the pathogenesis of bone metastasis formation in that disease. Antibodies have been developed that neutralize the action of PTHrP through its receptor, parathyroid hormone receptor 1, without influencing parathyroid hormone action through the same receptor. Such neutralizing antibodies against PTHrP are therapeutically effective in animal models of the humoral hypercalcemia of malignancy and of bone metastasis formation. We have determined the crystal structure of the complex between PTHrP (residues 1-108) and a neutralizing monoclonal anti-PTHrP antibody that reveals the only point of contact is an {alpha}-helical structure extending from residues 14-29. Another striking feature is that the same residues that interact with the antibody also interact with parathyroid hormone receptor 1, showing that the antibody and the receptor binding site on the hormone closely overlap. The structure explains how the antibody discriminates between the two hormones and provides information that could be used in the development of novel agonists and antagonists of their common receptor.

  10. Growth hormone releasing factor: comparison of two analogues and demonstration of hypothalamic defect in growth hormone release after radiotherapy.

    OpenAIRE

    Grossman, A; Lytras, N; Savage, M O; Wass, J. A.; Coy, D H; Rees, L. H.; Jones, A. E.; Besser, G M

    1984-01-01

    Human pancreatic growth hormone releasing factor (hpGHRF(1-40] stimulates the release of growth hormone in normal subjects and some patients with growth hormone deficiency. A study comparing the shorter chain amidated analogue hpGHRF(1-29) with an equivalent dose of hpGHRF(1-40) in seven normal subjects showed no significant difference in growth hormone response between the two preparations. Six patients with prolactinomas were also tested; these patients had received megavoltage radiotherapy...

  11. Hormonal changes in humans during spaceflight.

    Science.gov (United States)

    Strollo, F

    1999-01-01

    Readers of this review may feel that there is much more that we do not know about space endocrinology than what we know. Several reasons for this state of affairs have been given: 1. the complexity of the field of endocrinology with its still increasing number of known hormones, releasing factors and precursors, and of the interactions between them through various feedback mechanisms 2. the difficulty in separating the microgravity effects from the effects of stress from launch, isolation and confinement during flight, reentry, and postflight re-adaptation 3. the experimental limitations during flight, such as limited number of subjects, limited number of samples, impossibility of collecting triple samples for pulsatile hormones like growth hormone 4. the disturbing effects of countermeasures used by astronauts 5. the inadequacy of postflight samples for conclusions about inflight values 6. limitations of conclusions from animal experiments and space simulation studies The endocrinology field is divided in to nine systems or axes, which are successively reviewed: 1. Rapid bone demineralization in the early phase of spaceflight that, when unopposed, leads to catastrophic effects after three months but that slows down later. The endocrine mechanism, apart from the effect of exercise as a countermeasure, is not yet understood. 2. The hypothalamic-pituitary-adrenal axis is involved in stress reactions, which complicate our understanding and makes postflight analysis dubious. 3. In the hypothalamic-pituitary-gonadal axis, pulsatility poses a problem for obtaining representative values (e.g., for luteinizing hormone). Reproduction of rats in space is possible, but much more needs to be known about this aspect, particularly in women, before the advent of space colonies, but also in males because some evidence for reversible testicular dysfunction in space has been found. 4. The hypothalamic-pituitary-somato-mammotrophic axis involves prolactin and growth hormone. The

  12. Thyroid hormone receptor binds to a site in the rat growth hormone promoter required for induction by thyroid hormone

    International Nuclear Information System (INIS)

    Transcription of the rat growth hormone (rGH) gene in pituitary cells is increased by addition of thyroid hormone (T3). This induction is dependent on the presence of specific sequences just upstream of the rGH promoter. The authors have partially purified T3 receptor from rat liver and examined its interaction with these rGH sequences. They show here that T3 receptor binds specifically to a site just upstream of the basal rGH promoter. This binding site includes two copies of a 7-base-pair direct repeat, the centers of which are separated by 10 base pairs. Deletions that specifically remove the T3 receptor binding site drastically reduce response to T3 in transient transfection experiments. These results demonstrate that T3 receptor can recognize specific DNA sequences and suggest that it can act directly as a positive transcriptional regulatory factor

  13. Thyroid-stimulating Hormone (TSH): Measurement of Intracellular, Secreted, and Circulating Hormone in Xenopus laevis and Xenopus tropicalis.

    Science.gov (United States)

    Thyroid Stimulating Hormone (TSH) is a hormone produced in the pituitary that stimulates the thyroid gland to grow and produce thyroid hormone (TH). The concentration of TH controls developmental changes that take place in a wide variety of organisms. Many use the metaphoric ch...

  14. Luteinizing hormone-releasing hormone induces thyroxine release together with testosterone in the neotenic axolotl Ambystoma mexicanum.

    Science.gov (United States)

    Jacobs, G F; Kühn, E R

    1988-09-01

    In male neotenic axolotls Ambystoma mexicanum plasma concentrations of thyroxine (T4) and testosterone were increased following intravenous injection of 10 micrograms luteinizing hormone-releasing hormone. A dose of 50 micrograms influenced only plasma T4 levels. This observation suggests for the first time that a hypothalamic hormone is capable of stimulating the thyroidal axis in the neotenic axolotl.

  15. Pathology of excessive production of growth hormone.

    Science.gov (United States)

    Scheithauer, B W; Kovacs, K; Randall, R V; Horvath, E; Laws, E R

    1986-08-01

    Since its clinical description in the last century, much progress has been made in our understanding of acromegaly. From an initial description of pituitary enlargement as just another manifestation of generalized visceromegaly, the pituitary abnormality has come to be recognized, in most instances, as the underlying aetiological factor. Gigantism and acromegaly are manifestations of disordered pituitary physiology, but the lesion responsible may be hypothalamic, adenohypophyseal or ectopic in location. The best known pathological hypothalamic basis for acromegaly is represented by a neuronal malformation or 'gangliocytoma'. It usually takes the form of an intrasellar gangliocytoma or, more rarely, a hypothalamic hamartoma. The neuronal elaboration of GHRH may play a role in the development of a growth hormone adenoma; the pituitary process may pass through an intermediate stage of somatotropic hyperplasia. When acromegaly has its basis in a pituitary abnormality, the lesion is almost exclusively an adenoma; the non-tumorous adenohypophysis shows no evidence of coexistent hyperplasia. Surprisingly, such tumours are more often engaged in the formation of multiple hormones rather than GH alone. They frequently produce not only GH and prolactin, the products characteristics of cells of the acidophil line, but also glycoprotein hormones, usually TSH. The spectrum of adenomas also varies in its degree of differentiation from a histogenetically primitive lesion, the acidophil stem cell adenoma, to well-differentiated tumours of varying cellular composition and hormone content. Each adenoma type has its clinicopathological, histochemical, immunocytological and ultrastructural characteristics. The isolation and characterization of GHRH has permitted the identification of neuroendocrine tumours, most of foregut origin, elaborating this releasing hormone. Such functional tumours induce hyperplasia of pituitary somatotrophs and may, on occasion, result in the formation of

  16. The use of hormonal agents in the treatment of acne.

    Science.gov (United States)

    Hassoun, Lauren A; Chahal, Dev S; Sivamani, Raja K; Larsen, Larissa N

    2016-06-01

    Hormones and androgens play an important role in the pathogenesis of acne. Multiple hormonal modulators are now available for the treatment of acne. The efficacies and side effects of currently available hormonal agents are reviewed here including the use of oral contraceptives, spironolactone, flutamide, cyproterone acetate, finasteride, and cortexolone 17α-propionate. Hormonal therapies are an efficacious treatment option for acne among females. With the growing need to reduce antibiotic exposures, hormonal therapies should be more widely studied and incorporated into acne treatment strategies. PMID:27416311

  17. Regulation of Thyroid Hormone Bioactivity in Health and Disease

    OpenAIRE

    Peeters, Robin

    2005-01-01

    textabstractTThyroid hormone plays an essential role in a variety of metabolic processes in the human body. Examples are the effects of thyroid hormone on metabolism and on the heart. The production of thyroid hormone by the thyroid is regulated by thyroid stimulating hormone (TSH) via the TSH receptor. The thyroid produces T4, which is not biologically active. Therefore, T4 has to be converted to the active hormone T3, a process that is regulated by three enzymes, the deiodinases (D1-D3). D1...

  18. MicroRNA: sex steroids, hormonal carcinogenesis, hormonal sensitivity of tumor tissue

    Directory of Open Access Journals (Sweden)

    A. V. Malek

    2015-01-01

    Full Text Available Sex hormones, regulating normal physiological processes of most tissues and organs, are considered to be one of the key factors in the development and progression of the reproductive system cancer. Recently, the importance of the system for post-transcriptional control of gene expression mediated by short single-stranded RNA molecules (microRNA became evident. This system is involved in regulation of normal physiological processes and in the pathogenesis of many diseases, including cancer. In review we discuss the relationship between the two regulatory systems – sex hormones and microRNAs. The relationship of these systems is considered in the context of two tumors – breast and prostate cancer. In particular, the history of research on the role of sex hormones in the pathogenesis of breast cancer and prostate cancer is briefly covered. Additionally, modern scientific data on the biogenesis and biological role of microRNAs are presented in more detail. In the cells of the hormone-sensitive tissues, sex hormones regulate the microRNA-mediated machinery of gene expression control by two known ways: specifically, affecting the activity of individual microRNA molecules and non-specifically by altering the efficiency of microRNA biogenesis and activity of RNA-induced silencing complex. This downstream regulatory network substantially enhances biological effects of sex hormones at physiological conditions. Malignant transformation leads to a distortion of the regulatory effects of sex hormones that crucially influence the system of microRNA-regulated post-transcriptional control of gene expression. The most established and clinically significant example of such phenomenon is the loss of sensitivity of cells to the regulatory action of these hormones. As a consequence, cancer cells acquire the ability to active proliferation without stimulation with sex hormones. This effect is partly mediated by microRNAs. Also, relevant experimental data

  19. Investigation of sexual dimorphisms through mouse models and hormone/hormone-disruptor treatments.

    Science.gov (United States)

    Ipulan, Lerrie Ann; Raga, Dennis; Suzuki, Kentaro; Murashima, Aki; Matsumaru, Daisuke; Cunha, Gerald; Yamada, Gen

    2016-01-01

    Sexual dimorphism in mouse reproductive tissues is observable in adult, post-natal, and embryonic stages. The development of sexually dimorphic tissues starts with an ambisexual structure. It is followed by sex-specific organogenesis as guided by different signaling pathways that occur from late embryonic stages. The measurement of the anogenital distance (AGD), and the observation of the external genitalia are practical ways to distinguish male and female pups at birth and thereafter. Careful observation of the morphological or histological features and the molecular signatures of the external genitalia and perineum enable identification of sex or feminization/masculinization of embryos. Aberrations in hormone signaling via castration or treatment with hormones or hormone disruptors result in dysmorphogenesis of reproductive tissues. Several hormone disruptors have been used to modulate different aspects of hormone action through competitive inhibition and exogenous hormone treatment. Concomitantly, the vast advancement of conditional mutant mouse analysis leads to the frequent utilization of Cre recombination technology in the study of reproductive/urogenital tissue development. Mouse Cre-lines that are tissue-specific and cell-specific are also effective tools in identifying the molecular mechanisms during sexually dimorphic development. Cre-lines applicable to different cell populations in the prostate, seminal vesicles, testis and ovaries, and mammary glands are currently being utilized. In the external genitalia and perineum, Cre lines that examine the signaling pathways of cells of endodermal, ectodermal, and mesenchymal origin reveal the roles of these tissues in the development of the external genitalia. The interaction of hormones and growth factors can be examined further through a variety of techniques available for researchers. Such cumulative information about various technologies is summarized. PMID:26651426

  20. Regulation of gut hormone secretion. Studies using isolated perfused intestines

    DEFF Research Database (Denmark)

    Svendsen, Berit; Holst, Jens Juul.

    2016-01-01

    A review. The incretin hormones glucagon-like peptide 1 (GLP-1) and glucose-dependent insulinotropic polypeptide (GIP) are secreted from enteroendocrine cells in the intestine along with other gut hormones (PYY, CCK and neurotensin) shown to affect metab. and/or appetite. The secretion of many gut...... hormones is highly increased after gastric bypass operations, which have turned out to be an effective therapy of not only obesity but also type 2 diabetes. These effects are likely to be due, at least in part, to increases in the secretion of these gut hormones (except GIP). Therefore, stimulation...... of the endogenous hormone represents an appealing therapeutic strategy, which has spurred an interest in understanding the regulation of gut hormone secretion and a search for particularly GLP-1 and PYY secretagogues. The secretion of the gut hormones is stimulated by oral intake of nutrients often including...

  1. Psychopathology and hormonal disturbances in eating disorders

    Directory of Open Access Journals (Sweden)

    Pierpaola D’Arista

    2008-09-01

    Full Text Available

    Background: Our aim was to study the relationship between hormonal disturbances and psychopathology in Eating Disorders (ED.

    Methods: Forty-nine women diagnosed as Eating Disorders according to DSM-IV were subjected to control plasma levels of TSH, FT3, FT4, LH, FSH, 17beta-estradiol, prolactin, cortisol, DHEAS, GH and IGF-1. They were also administered by SCL-90R, BAT, DES II questionnaires. We applied multivariate regression models.

    Results: Our results highlight a statistically significant relation between LH, FSH and prolactin decreased levels, mood and thought disturbances (subscales 3, 5, 7, 8 and 9 of SCL-90r which are associated to Body Attitude ( BAT total scale and Dissociative Experiences (DES II total scale.

    Conclusions: Decreased sexual hormones levels could have a role in ED psychological disturbances, not inquired yet

  2. Dimerization of Human Growth Hormone by Zinc

    Science.gov (United States)

    Cunningham, Brian C.; Mulkerrin, Michael G.; Wells, James A.

    1991-08-01

    Size-exclusion chromatography and sedimentation equilibrium studies demonstrated that zinc ion (Zn2+) induced the dimerization of human growth hormone (hGH). Scatchard analysis of 65Zn2+ binding to hGH showed that two Zn2+ ions associate per dimer of hGH in a cooperative fashion. Cobalt (II) can substitute for Zn2+ in the hormone dimer and gives a visible spectrum characteristic of cobalt coordinated in a tetrahedral fashion by oxygen- and nitrogen-containing ligands. Replacement of potential Zn2+ ligands (His18, His21, and Glu174) in hGH with alanine weakened both Zn2+ binding and hGH dimer formation. The Zn2+-hGH dimer was more stable than monomeric hGH to denaturation in guanidine-HCl. Formation of a Zn2+-hGH dimeric complex may be important for storage of hGH in secretory granules.

  3. Hormonal regulation of the hypothalamic melanocortin system

    Directory of Open Access Journals (Sweden)

    Jung Dae eKim

    2014-12-01

    Full Text Available Regulation of energy homeostasis is fundamental for life. In animal species and humans, the Central Nervous System (CNS plays a critical role in such regulation by integrating peripheral signals and modulating behavior and the activity of peripheral organs. A precise interplay between CNS and peripheral signals is necessary for the regulation of food intake and energy expenditure in the maintenance of energy balance. Within the CNS, the hypothalamus is a critical center for monitoring, processing and responding to peripheral signals, including hormones such as ghrelin, leptin and insulin. Once in the brain, peripheral signals regulate neuronal systems involved in the modulation of energy homeostasis. The main hypothalamic neuronal circuit in the regulation of energy metabolism is the melanocortin system. This review will give a summary of the most recent discoveries on the hormonal regulation of the hypothalamic melanocortin system in the control of energy homeostasis.

  4. The pituitary growth hormone cell in space

    Science.gov (United States)

    Hymer, Wesley C.; Grindeland, R.

    1989-01-01

    Growth hormone (GH), produced and secreted from specialized cells in the pituitary gland, controls the metabolism of protein, fat, and carbohydrate. It is also probably involved in the regulation of proper function of bone, muscle and immune systems. The behavior of the GH cell system was studied by flying either isolated pituitary cells or live rats. In the latter case, pituitary GH cells are prepared on return to earth and then either transplanted into hypophysectomized rats or placed into cell culture so that function of GH cells in-vivo vs. in-vitro can be compared. The results from three flights to date (STS-8, 1983; SL-3, 1985; Cosmos 1887, 1987) established that the ability of GH cells to release hormone, on return to earth, is compromised. The mechanism(s) responsible for this attenuation response is unknown. However, the data are sufficiently positive to indicate that the nature of the secretory defect resides directly within the GH cells.

  5. Hormonal changes during long-term isolation.

    Science.gov (United States)

    Custaud, M A; Belin de Chantemele, E; Larina, I M; Nichiporuk, I A; Grigoriev, A; Duvareille, M; Gharib, C; Gauquelin-Koch, G

    2004-05-01

    Confinement and inactivity induce considerable psychological and physiological modifications through social and sensory deprivation. The aim of the SFINCSS-99 experiment was to determine the cardiovascular and hormonal pattern of blood volume regulation during long-term isolation and confinement. Simulation experiments were performed in pressurized chambers similar in size to the volumes of modern space vehicles. Group I consisted of four Russian male volunteers, who spent 240 days in a 100-m(3 )chamber. Group II included four males (one German and three Russians) who spent 110 days in isolation (200-m(3) module). The blood samples, taken before, during and after the isolation period, were used to determine haematocrit (Ht), growth hormone (GH), active renin, aldosterone, and osmolality levels. From the urine samples, electrolytes, osmolality, nitrites, nitrates, cortisol, antidiuretic hormone (ADH), aldosterone, normetanephrine and metanephrine levels were determined. The increase in plasma volume (PV) that is associated with a tendency for a decrease in plasma active renin is likely to be due to decreased sympathetic activity, and concords with the changes in urinary catecholamine levels during confinement. Urinary catecholamine levels were significantly higher during the recovery period than during confinement. This suggests that the sympathoadrenal system was activated, and concords with the increase in heart rate. Vascular resistance is determined by not only the vasoconstrictor but also vasodilator systems. The ratio of nitrite/nitrate in urine, as an indicator of nitric oxide release, did not reveal any significant changes. Analysis of data suggests that the duration of the isolation was a main factor involved in the regulation of hormones.

  6. Gender, sex hormones and pulmonary hypertension

    OpenAIRE

    Austin, Eric D.; Johansen, Anne Katrine; Alzoubi, Abdallah; Lahm, Tim; West, James; Tofovic, Stevan P.; MacLean, Margaret R.; Oka, Masahiko

    2013-01-01

    Most subtypes of pulmonary arterial hypertension (PAH) are characterized by a greater susceptibility to disease among females, although females with PAH appear to live longer after diagnosis. While this “estrogen paradoxȍ of enhanced female survival despite increased female susceptibility remains a mystery, recent progress has begun to shed light upon the interplay of sex hormones, the pathogenesis of pulmonary hypertension, and the right ventricular response to stress. For example, emerging ...

  7. TPR Proteins in Plant Hormone Signaling

    OpenAIRE

    Schapire, Arnaldo L; Valpuesta, Victoriano; Botella, Miguel A

    2006-01-01

    There is a large number of proteins in nature containing Tetratrico Peptide Repeats (TPRs). TPR motifs are defined as a protein-protein interaction module involved in regulation of different cellular functions. We have recently identified TTL1 as a protein containing TPR motifs required for abscisic acid responses and osmotic stress tolerance. In recent years several of these proteins have been found to be essential for responses to other hormones such ethylene, cytokinin, gibberelling and au...

  8. Strigolactones: new plant hormones in action

    OpenAIRE

    Zwanenburg, Binne; Pospíšil, Tomáš; Ćavar Zeljković, Sanja

    2016-01-01

    Main conclusion The key step in the mode of action of strigolactones is the enzymatic detachment of the D-ring. The thus formed hydroxy butenolide induces conformational changes of the receptor pocket which trigger a cascade of reactions in the signal transduction. Abstract Strigolactones (SLs) constitute a new class of plant hormones which are of increasing importance in plant science. For the last 60 years, they have been known as germination stimulants for parasitic plants. Recently, sever...

  9. Mechanisms of brassinosteroids interacting with multiple hormones

    OpenAIRE

    Zhang, Shanshan; Wei, Ying; Lu, Yangning; Wang, Xuelu

    2009-01-01

    Various environmental and internal cues play essential roles in regulating diverse aspects of plant growth and development. Phytohormones usually coordinate multiple stimuli to directly regulate multiple developmental programs. Recent studies have provided progresses into the complexity of their cross talk. Particularly, the signaling pathways of various phytohormones have been revealed, leading to the discovery of the mechanisms of the interplay among different hormone signaling pathways. Th...

  10. Growth hormone and aging: A challenging controversy

    OpenAIRE

    Andrzej Bartke

    2008-01-01

    Andrzej BartkeGeriatrics Research, Departments of Internal Medicine and Physiology, Southern Illinois University School of Medicine, Springfield, IL, USAAbstract: Although advanced age or symptoms of aging are not among approved indications for growth hormone (GH) therapy, recombinant human GH (rhGH) and various GH-related products are aggressively promoted as anti-aging therapies. Well-controlled studies of the effects of rhGH treatment in endocrinologically normal elderly subjects report so...

  11. Justified and unjustified use of growth hormone

    OpenAIRE

    van der Lely, A J

    2004-01-01

    Growth hormone (GH) replacement therapy for children and adults with proven GH deficiency due to a pituitary disorder has become an accepted therapy with proven efficacy. GH is increasingly suggested, however, as a potential treatment for frailty, osteoporosis, morbid obesity, cardiac failure, and various catabolic conditions. However, the available placebo controlled studies have not reported many significant beneficial effects, and it might even be dangerous to use excessive GH dosages in c...

  12. Sex hormonal modulation of interhemispheric transfer time.

    OpenAIRE

    Hausmann, M; Hamm, J. P.; K. E. Waldie; Kirk, I. J.

    2013-01-01

    It is still a matter of debate whether functional cerebral asymmetries (FCA) of many cognitive processes are more pronounced in men than in women. Some evidence suggests that the apparent reduction in women's FCA is a result of the fluctuating levels of gonadal steroid hormones over the course of the menstrual cycle, making their FCA less static than for men. The degree of lateralization has been suggested to depend on interhemispheric communication that may be modulated by gonadal steroid ho...

  13. Men's perspectives of male hormonal contraception

    Directory of Open Access Journals (Sweden)

    Alison L. Lloyd

    2016-08-01

    Conclusions: This study was successful in its aim, finding that overall MHC would be well received by men and that their perspectives were not that different from attitudes towards female hormone contraception. It also identified potential barriers based on the concerns that men have for themselves and for society were an MHC to become available. [Int J Reprod Contracept Obstet Gynecol 2016; 5(8.000: 2546-2552

  14. NUREBASE: database of nuclear hormone receptors

    OpenAIRE

    Duarte, Jorge; Perrière, Guy; Laudet, Vincent; Robinson-Rechavi, Marc

    2002-01-01

    Nuclear hormone receptors are an abundant class of ligand activated transcriptional regulators, found in varying numbers in all animals. Based on our experience of managing the official nomenclature of nuclear receptors, we have developed NUREBASE, a database containing protein and DNA sequences, reviewed protein alignments and phylogenies, taxonomy and annotations for all nuclear receptors. The reviewed NUREBASE is completed by NUREBASE_DAILY, automatically updated every 24 h. Both databases...

  15. The effect of anti-müllerian hormone of serum and follicular fluid in vitro fertilization-embryo transfer%血清及卵泡液中的抗苗勒氏管激素在体外受精-胚胎移植中的作用

    Institute of Scientific and Technical Information of China (English)

    2016-01-01

    Objective To comprae the difference of Sex hormone,serum and follicular fluid anti Mullerian hormone (AMH) level tube,number of retrieved egg,the number of cleavage rate,fertilization oocytes,the number of available embryos and total embryo on in vitro fertilization and embryo transfer (IVF-ET) of different reaction groups,to explore the relationship between AMH and pregnancy outcome of IVF-EH.Methods Eighty-one cases of IVF-ET assisted pregnancy were divided into low ovarian response group,normal ovarian response group and high ovarian response group according to the response of the super ovulation.Serum sex hormone and AMH of all patients were detected on the third day of menstruation,on the day of HCG injection,oocyte pick-up (OPU) and embryo transfer (ET),AMH in FF were detected too.The difference of oocytes,cleavage rate,embryo quality and the indicators mentioned above among the three groups were compared.All of the above indicators were compared between pregnancy group and unpregnancy group.Results (1) The level of AMH on the third day of menstruation ((1.76 ± 0.47) μg/L,(3.45 ± 1.01) μg/L,(6.34 ± 1.29) μg/L,F =2.435,P<0.001),HCG injection ((1.09±0.13)μg/L,(1.29±0.29)μg/L,(2.64±0.51)μg/L,F=1.542,P=0.001) and embryo planting((1.32±0.33) μg/L,(1.62±0.39) μg/L,(1o 91±0.41) μg/L,F =1.573,P <0.001) all were statistically significant in three groups.On day of OPU,the levels of serum AMH ((0.95±0.21)μg/L,(1.15±0.29) μg/L,(1.74±0.41) μg/L,F =12.573,P<0.001) and AMH in FF((5.82±1.19) μg/L,(6.92±1.05) μg/L,(7.79±1.39) μg/L,F =9.83,P<0.001) were statistically differences in the three groups.(2)The number of oocytes were positively correlated with AFC,based AMH,levels of E2,P and AMH on the day of HCG injection and OPU,E2 and AMH on the day of ET and AMH in FF(P<0.05).(3)AMH in FF in pregnancy group was (6.22±0.82)μ g/L,significantly higher than those without pregnancy group ((5.31 ±0.71)μg/L,P =0.037).Conclusions Levels of

  16. Exercise and the Regulation of Endocrine Hormones.

    Science.gov (United States)

    Hackney, Anthony C; Lane, Amy R

    2015-01-01

    The endocrine system has profound regulatory effects within the human body and thus the ability to control and maintain appropriate function within many physiological systems (i.e., homeostasis). The hormones associated with the endocrine system utilize autocrine, paracrine, or endocrine actions on the cells of their target tissues within these physiologic systems to adjust homeostasis. The introduction of exercise as a stressor to disrupt homeostasis can greatly amplify and impact the actions of these hormones. To that end, the endocrine response to an acute exercise session occurs in a progression of phases with the magnitude of the response being relative to the exercise work intensity or volume. Various physiologic mechanisms are considered responsible for these responses, although not all are completely understood or elucidated. Chronic exercise training does not eliminate the acute exercise response but may attenuate the overall effect of the responsiveness as the body adapts in a positive fashion to the training stimulus. Regrettably, an excessive intensity and/or volume of training may lead to maladaptation and is associated with inappropriate endocrine hormonal responses. The mechanisms leading to a deleterious maladaptive state are not well understood and require additional research for elucidation. PMID:26477919

  17. [Metastatic hormone-sensitive prostate cancer].

    Science.gov (United States)

    Gravis, Gwenaelle; Salem, Naji; Walz, Jochen

    2015-01-01

    The prostate cancer in its hormone-sensitive metastatic presentation is infrequent, it is either an initial presentation of the disease or an evolution after local treatment, without castration of the biological relapse. The surgical or biological castration remains the cornerstone of the treatment. The deadline of castration initiation and its modalities of administration, intermittent or continuous rest debated but consensual on the initiation is the appearance of the symptomatic disease. The chemotherapy by docetaxel in association with the castration increases significantly the survival of the patients having a high tumoral volume. The efficacy on the whole metastatic population requires additional analyses. Clinical prognostic factors as the bone localizations (axial or appendicular), the visceral involvement (liver, lung) are determining for the survival of these patients. Biological prognostic factors are in evaluation. Except the clodronate acid, which showed a survival improvement in the hormone-sensitive metastatic prostate cancer (HSMPC), the other treatments targeting the bone (zoledronic acid, rank-ligand inhibitor) demonstrated a benefit only in castrate resistant metastatic prostate cancer (MCRPC). The management of local disease lets suggest a benefit to at least symptomatic disease, but it requires to be estimated prospectively in clinical trials. The new hormonal treatments targeting the androgen receptor in CPMRC are in evaluation in CPMHS. The objective is to increase the survival and the quality of life of the CPMHS and to delay the evolution towards the castration resistant metastatic disease. PMID:25609491

  18. Active acromegaly enhances spontaneous parathyroid hormone pulsatility.

    Science.gov (United States)

    Mazziotti, Gherardo; Cimino, Vincenzo; De Menis, Ernesto; Bonadonna, Stefania; Bugari, Giovanna; De Marinis, Laura; Veldhuis, Johannes D; Giustina, Andrea

    2006-06-01

    In healthy subjects, parathyroid hormone (PTH) is secreted in a dual fashion, with low-amplitude and high-frequency pulses superimposed on tonic secretion. These 2 components of PTH secretion seem to have different effects on target organs. The aim of our study was to evaluate whether growth hormone excess in acromegaly may modify the spontaneous pulsatility of PTH. Five male patients with newly diagnosed active acromegaly and 8 healthy subjects were evaluated by 3-minute blood sampling for 6 hours. Plasma PTH concentrations were evaluated by multiparameter deconvolution analysis. Plasma PTH release profiles were also subjected to an approximate entropy (ApEn) estimate, which provides an ensemble measure of the serial regularity or orderliness of the release process. In acromegalic patients, baseline serum PTH values were not significantly different from those measured in the healthy subjects, as well as tonic PTH secretion rate, number of bursts, fractional pulsatile PTH secretion, and ApEn ratio. Conversely, PTH pulse half-duration was significantly longer in acromegalic patients vs healthy subjects (11.8+/-0.95 vs 6.9+/-1.6 minutes; P=.05), whereas PTH pulse mass showed a tendency (P=.06) to be significantly greater in acromegalic patients. These preliminary data suggest that growth hormone excess may affect PTH secretory dynamics in patients with acromegaly. Potentially negative bone effects of the modifications of PTH secretory pattern in acromegaly should be investigated.

  19. The current state of male hormonal contraception.

    Science.gov (United States)

    Chao, Jing H; Page, Stephanie T

    2016-07-01

    World population continues to grow at an unprecedented rate, doubling in a mere 50years to surpass the 7-billion milestone in 2011. This steep population growth exerts enormous pressure on the global environment. Despite the availability of numerous contraceptive choices for women, approximately half of all pregnancies are unintended and at least half of those are unwanted. Such statistics suggest that there is still a gap in contraceptive options for couples, particularly effective reversible contraceptives for men, who have few contraceptive choices. Male hormonal contraception has been an active area of research for almost 50years. The fundamental concept involves the use of exogenous hormones to suppress endogenous production of gonadotropins, testosterone, and downstream spermatogenesis. Testosterone-alone regimens are effective in many men but high dosing requirements and sub-optimal gonadotropin suppression in 10-30% of men limit their use. A number of novel combinations of testosterone and progestins have been shown to be more efficacious but still require further refinement in delivery systems and a clearer understanding of the potential short- and long-term side effects. Recently, synthetic androgens with both androgenic and progestogenic activity have been developed. These agents have the potential to be single-agent male hormonal contraceptives. Early studies of these compounds are encouraging and there is reason for optimism that these may provide safe, reversible, and reliable contraception for men in the near future. PMID:27016468

  20. Hormone activation of baculovirus expressed progesterone receptors.

    Science.gov (United States)

    Elliston, J F; Beekman, J M; Tsai, S Y; O'Malley, B W; Tsai, M J

    1992-03-15

    Human and chicken progesterone receptors (A form) were overproduced in a baculovirus expression system. These recombinant progesterone receptors were full-length bound progesterone specifically and were recognized by monoclonal antibodies, AB52 and PR22, specific for human and chicken progesterone receptor, respectively. In gel retardation studies, binding of recombinant human and chicken progesterone receptors to their progesterone response element (PRE) was specific and was enhanced in the presence of progesterone. Binding of human progesterone receptor to the PRE was also enhanced in the presence of the antiprogestin, RU486, but very little effect was observed in the presence of estradiol, dexamethasone, testosterone, and vitamin D. In our cell-free transcription system, human progesterone receptor induced transcription in a receptor-dependent and hormone-activable manner. Receptor-stimulated transcription required the presence of the PRE in the test template and could be specifically inhibited by excess PRE oligonucleotides. Furthermore, chicken progesterone receptor also induced in vitro transcription in a hormone-activable manner. These results demonstrate that steroid receptors overexpressed in a baculovirus expression system are functional and exhibit steroid-responsive binding and transcription. These observations support our present understanding of the mechanism of steroid receptor-regulated gene expression and provide a technological format for studies of the role of hormone and antihormone in altering gene expression. PMID:1544902

  1. Transport of thyroid hormone in brain

    Directory of Open Access Journals (Sweden)

    Eva K Wirth

    2014-06-01

    Full Text Available Thyroid hormone (TH transport into the brain is not only pivotal for development and differentiation, but also for maintenance and regulation of adult central nervous system (CNS function. In this review, we highlight some key factors and structures regulating thyroid hormone uptake and distribution. Serum TH binding proteins play a major role for the availability of TH since only free hormone concentrations may dictate cellular uptake. One of these proteins, transthyretin is also present in the cerebrospinal fluid (CSF after being secreted by the choroid plexus. Entry routes into the brain like the blood-brain-barrier (BBB and the blood-CSF-barrier will be explicated regarding fetal and adult status. Recently identified TH transmembrane transporters (THTT like monocarboxylate transporter 8 (Mct8 play a major role in uptake of TH across the BBB but as well in transport between cells like astrocytes and neurons within the brain. Species differences in transporter expression will be presented and interference of TH transport by endogenous and exogenous compounds including endocrine disruptors and drugs will be discussed.

  2. HORMONAL EVALUATION IN FEMALES HAVING MELASMA

    Directory of Open Access Journals (Sweden)

    Sharique

    2015-09-01

    Full Text Available BACKGROUND: Melasma is a commonly acquired hyperpigmentation which present as irregular, light to dark brown macules on sun exposed skin due to various etiological factors including hormonal imbalance. AIM : To assist the level of various hormones and study the clinical and hormonal correlation in patients of melasma. METHODS : 50 female patients of melasma between age group 18 - 50 with equal number of age matched females with no signs of melasma, hirsutism and any other endocrinal abnormality, were enrolled. They were examined by woods’ lamp to see the type of melasma whether epidermal, dermal or mixed. 10 ml of venous blood sample was drawn after overnight fasting on 3 rd - 5 th day of the menstrual cycle in mid follicular phase for the assessment of LH, FSH, Prolactin, Estradiol and Progesterone by chemiluminescence method. RESULT : Out of 50 patients, 8 patients had deranged level of LH, 7 patients had deranged level of FSH, 14 patients had deranged prolactein, 18 patients had deranged estradiol and 6 patients had deranged level of progesterone. 70% patients were married and belong to age group of 31 - 40 years. 18 % patients has onset of melasma during pregnancy while 52% patients after the delivery. CONCLUSIONS : LH, estradiol and progesterone are found to be contributory factors in development of melasma.

  3. Biochemical endpoints of glucocorticoid hormone action

    Energy Technology Data Exchange (ETDEWEB)

    Young, D.A.; Nicholson, M.L.; Guyette, W.A.; Giddings, S.J.; Mendelsohn, S.L.; Nordeen, S.K.; Lyons, R.T.

    1978-01-01

    Both the rapidly evolving metabolic effects of glucocorticoids and the more slowly developing lethal actions appear to be initiated via the synthesis of new mRNAs and proteins. The chronic suppression of cell growth may be the consequence of suppression of overall rates of protein synthesis (and probably RNA and DNA synthesis as well) that in turn may represent the cellular response to the small changes in ratios of adenine nucleotides that result from the suppression of oxidative ATP production. The inhibition of glucose transport may also play a role here to prevent a compensatory increase in glycolytic ATP production. Some other hormone actions, the decrease in the ability of cells to concentrate AIB and the increase in nuclear fragility are unrelated to, and evolve separately from, the hormonal inhibitions on energy production. Cell killing is not the result of suppression of protein synthesis, nor of hormone-induced increases in calcium uptake. While the mechanisms are unknown, the increase in nuclear fragility appears to be the earliest measure of their operation. In tumor cells resistance to lethal actions of glucocorticoids may emerge via the selection of cells with hardier membranes, that are better able to withstand the intracellular destructive events set in motion by high levels of glucocorticoids.

  4. Thyroid hormones and postembryonic development in amniotes.

    Science.gov (United States)

    Holzer, Guillaume; Laudet, Vincent

    2013-01-01

    In chordates, metamorphosis is a developmental event well described in amphibians in which thyroid hormone triggers this event. Interestingly, among amphibians, several variations upon the eggs/tadpole/frog developmental sequence are observed such as direct development or neoteny. The fact that TH-regulated metamorphosis is conserved in invertebrate chordates such as amphioxus implies that this event is an ancient feature of all vertebrates. This allows us to propose that TH may play an important role in coordinating the postembryonic development of apparently nonmetamorphosing vertebrates such as mammals or sauropsids. Indeed, the observations of thyroid hormone levels in mammals and sauropsids draw interesting parallels with what is observed during amphibian metamorphosis. At the physiological level, the increase of thyroid hormone signaling is required for the normal development particularly for the intestine and the brain. At the behavioral level, a peak of TH often precedes the autonomy of the young from parental care. At the ecological level, offspring with a TH peak close to birth/hatching tends to be precocial young whereas offspring with a TH peak long after birth/hatching tends to be altricial young. Taken together, these observations in amniotes, which are not considered as undergoing metamorphosis during their development, are consistent with the idea of a late developmental step controlled by TH and allowing the accession to the adult ecological niche. Thus, according to this view, at the molecular level all vertebrates undergo a period of remodeling controlled by TH that is reminiscent of metamorphosis. PMID:23347527

  5. Hormonal and non-hormonal bases of maternal behavior: The role of experience and epigenetic mechanisms.

    Science.gov (United States)

    Stolzenberg, Danielle S; Champagne, Frances A

    2016-01-01

    This article is part of a Special Issue "Parental Care". Though hormonal changes occurring throughout pregnancy and at the time of parturition have been demonstrated to prime the maternal brain and trigger the onset of mother-infant interactions, extended experience with neonates can induce similar behavioral interactions. Sensitization, a phenomenon in which rodents engage in parental responses to young following constant cohabitation with donor pups, was elegantly demonstrated by Rosenblatt (1967) to occur in females and males, independent of hormonal status. Study of the non-hormonal basis of maternal behavior has contributed significantly to our understanding of hormonal influences on the maternal brain and the cellular and molecular mechanisms that mediate maternal behavior. Here, we highlight our current understanding regarding both hormone-induced and experience-induced maternal responsivity and the mechanisms that may serve as a common pathway through which increases in maternal behavior are achieved. In particular, we describe the epigenetic changes that contribute to chromatin remodeling and how these molecular mechanisms may influence the neural substrates of the maternal brain. We also consider how individual differences in these systems emerge during development in response to maternal care. This research has broad implications for our understanding of the parental brain and the role of experience in the induction of neurobiological and behavior changes. PMID:26172856

  6. Recovery of hormone sensitivity after salvage brachytherapy for hormone refractory localized prostate cancer

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    Dan Smith

    2010-06-01

    Full Text Available PURPOSE: Recent work has demonstrated the return of hormone sensitivity after palliative chemotherapy in androgen independent prostate cancer. We wished to establish whether a similar phenomenon existed in patients with no exposure to chemotherapy. MATERIALS AND METHODS: A review of “hormone resistant” patients who had received salvage brachytherapy for localized prostate cancer after previous external beam radiotherapy was undertaken. Three patients with subsequent biochemical relapse responded to the rechallenge with hormonal treatment. RESULTS: The series of patients presented here demonstrates this phenomenon occurs after salvage brachytherapy with no exposure to chemotherapy. Recovery of sensitivity is demonstrated both to androgen deprivation and to androgen receptor antagonism. The recovery of hormone sensitivity was surprisingly durable, ranging from eight months to over twenty-one months. CONCLUSIONS: Hormone sensitivity may be recovered after salvage brachytherapy. Potential mechanisms underlying these observations are discussed and the likely central role of the activity of the androgen receptor highlighted. The relevance of these findings to the management of advanced prostate cancer is considered including thoughts on the practice of intermittent anti-androgen therapy.

  7. Effect of hypothyroidism on female reproductive hormones

    Directory of Open Access Journals (Sweden)

    Sanjay Saran

    2016-01-01

    Full Text Available Objective: Objective was to evaluate reproductive hormones levels in hypothyroid women and impact of treatment on their levels. Materials and Methods: A total of 59 women with untreated primary hypothyroidism were included in this prospective study. Venous blood was taken at baseline and after euthyroidism was achieved for measuring serum free thyroxine, free triiodothyronine (FT3, thyroid stimulating hormone (TSH, prolactin (PRL, follicular stimulating hormone (FSH, luteinizing hormone (LH, estradiol (E2, testosterone (T, and thyroid peroxidase antibody. Thirty-nine healthy women with regular menstrual cycles without any hormonal disturbances served as controls. The statistical analysis was performed using the Statistical Package for the Social Sciences Version 20 ([SPSS] IBM Corporation, Armonk, NY, USA. P < 0.05 was considered statistically significant. Results: On an average at diagnosis cases have more serum TSH (mean[M] = 77.85; standard error [SE] = 11.72, PRL (M = 39.65; SE = 4.13 and less serum E2(M = 50.00; SE = 2.25 and T (M = 35.40; SE = 2.31 than after achieving euthyroidism (M = 1.74; SE = 0.73, (M = 16.04; SE = 0.84, (M = 76.25; SE = 2.60, and (M = 40.29; SE = 2.27, respectively. This difference was statistically significant t(58 = 6.48, P <0.05; t(58 = 6.49, P < 0.05; t(58 = 12.47; P <0.05; and t(58 = 2.04, P <0.05; respectively. Although average serum FSH(M = 12.14; SE = 0.40 and LH (M = 5.89; SE = 0.27 were lower in cases at diagnosis than after achieving euthyroidism (M = 12.70; SE = 0.40,(M = 6.22; SE = 0.25, respectively, but these differences were statistically insignificant t(58 = 1.61, P = 0.11; t(58 = 1.11, P = 0.27, respectively. Conclusion: The study has demonstrated low E2 and T levels in hypothyroid women which were increased after achieving euthyroidism. Although average serum FSH and LH were increased in hypothyroid women after achieving euthyroidism but this difference was statistically insignificant.

  8. Practice of Consanguinity and Unusual Cases of Inherited Familial Chromosome Abnormalities: A Case Report.

    Science.gov (United States)

    Sanyal, Debarshi; Bhairi, Vidya; S Kadandale, Jayarama

    2016-01-01

    We present 2 cases of likely rare event. In case 1, 3(rd) degree consanguineous marriage revealed inv(6) with same break points in parents who were found to be phenotypically normal. The same inv(6) being inherited in progeny but presented with low AMH (anti Mullerian hormone) and high level of FSH (follicular stimulating hormone) leading to polycystic ovarian syndrome/premature ovarian failure. In case 2, a couple was presented with 2(nd) degree consanguineous marriage and referred for 2 recurrent/ missed abortions. The amounts of shared genes are suggestive of more lethal genetic outcomes and inferred endogamy is a major driver to reproductive fiascoes, the ancestries of which are deeply tied at the meiotic level. PMID:27386439

  9. Immunoreactive luteinizing hormone-releasing hormone in the seminal plasma and human semen parameters

    International Nuclear Information System (INIS)

    A luteinizing hormone-releasing hormone (LH-RH)-like substance has been detected in human seminal plasma by a radioimmunoassay (RIA) with a highly specific anti-LH-RH antiserum. The seminal samples - not only the plasma itself but also the sample extracted by an acid/alcohol method - showed satisfactory displacement curves in our RIA system. The relationship between fertility and the LH-RH values in the seminal plasma was studied by comparing the peptide levels with sperm concentration and motility. By these two parameters, 103 samples were divided into four groups. In the low-concentration groups (oligozoospermic patients), the hormonal concentrations differed significantly between those specimens demonstrating good and poor motility. These data suggest that this immunoreactive LH-RH may play a role in human spermatogenesis

  10. Growth hormone treatment during pregnancy in a growth hormone-deficient woman

    DEFF Research Database (Denmark)

    Müller, J; Starup, J; Christiansen, J S;

    1995-01-01

    Information on the course and outcome of pregnancies in growth hormone (GH)-deficient patients is sparse, and GH treatment during pregnancy in such women has not been described previously. We have studied fetal growth and serum levels of GH, insulin-like growth factor I (IGF-I) and IGF binding...... protein 3 (IGFBP-3) during pregnancy, as well as birth weight and hormone levels after delivery in a 25-year-old woman with idiopathic, isolated GH deficiency diagnosed at the age of 7 years. As part of a clinical trial, the patient was treated with 2 IU/M2 GH for a period of 5 years. At this time she...... became pregnant after donor insemination. The GH treatment was continued until variant GH production from the placenta was evident. Serum levels of GH, IGF-I and IGFBP-3 were measured monthly during pregnancy after 3 days off GH therapy. Abdominal ultrasound was performed five times. Hormonal levels were...

  11. Diagnosis and treatment of infertility-related male hormonal dysfunction.

    Science.gov (United States)

    Kathrins, Martin; Niederberger, Craig

    2016-06-01

    Treatment of infertility-related hormonal dysfunction in men requires an understanding of the hormonal basis of spermatogenesis. The best method for accurately determining male androgenization status remains elusive. Treatment of hormonal dysfunction can fall into two categories - empirical and targeted. Empirical therapy refers to experience-based treatment approaches in the absence of an identifiable aetiology. Targeted therapy refers to the correction of a specific underlying hormonal abnormality. However, the tools available for inferring the intratesticular hormonal environment are unreliable. Thus, understanding the limitations of serum hormonal assays is very important for determining male androgen status. Furthermore, bulk seminal parameters are notoriously variable and consequently unreliable for measuring responses to hormonal therapy. In the setting of azoospermia owing to spermatogenic dysfunction, hormonal therapy - relying on truly objective parameters including the return of sperm to the ejaculate or successful surgical sperm retrieval - is a promising treatment. This approach to the treatment of fertility-related hormonal dysfunction in men contrasts with the current state of its counterpart in female reproductive endocrinology. Treatment of male hormonal dysfunction has long emphasized empirical therapy, whereas treatment of the corollary female dysfunction has been directed at specific deficits. PMID:27091665

  12. The reciprocal regulation of stress hormones and GABAA receptors

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    Istvan eMody

    2012-01-01

    Full Text Available Stress-derived steroid hormones regulate the expression and function of GABAA receptors (GABAARs. Changes in GABAAR subunit expression have been demonstrated under conditions of altered steroid hormone levels, such as stress, as well as following exogenous steroid hormone administration. In addition to the effects of stress-derived steroid hormones on GABAAR subunit expression, stress hormones can also be metabolized to neuroactive derivatives which can alter the function of GABAARs. Neurosteroids allosterically modulate GABAARs at concentrations comparable to those during stress. In addition to the actions of stress-derived steroid hormones on GABAARs, GABAARs reciprocally regulate the production of stress hormones. The stress response is mediated by the hypothalamic-pituitary-adrenal (HPA axis, the activity of which is governed by corticotropin releasing hormone (CRH neurons. The activity of CRH neurons is largely controlled by robust GABAergic inhibition. Recently, it has been demonstrated that CRH neurons are regulated by neurosteroid-sensitive, GABAAR δ subunit-containing receptors representing a novel feedback mechanism onto the HPA axis. Further, it has been demonstrated that neurosteroidogenesis and neurosteroid actions on GABAAR δ subunit-containing receptors on CRH neurons are necessary to mount the physiological response to stress. Here we review the literature describing the effects of steroid hormones on GABAARs as well as the importance of GABAARs in regulating the production of steroid hormones. This review incorporates what we currently know about changes in GABAARs following stress and the role in HPA axis regulation.

  13. Sex hormones, sex hormone binding globulin, and vertebral fractures in older men.

    Science.gov (United States)

    Cawthon, Peggy M; Schousboe, John T; Harrison, Stephanie L; Ensrud, Kristine E; Black, Dennis; Cauley, Jane A; Cummings, Steven R; LeBlanc, Erin S; Laughlin, Gail A; Nielson, Carrie M; Broughton, Augusta; Kado, Deborah M; Hoffman, Andrew R; Jamal, Sophie A; Barrett-Connor, Elizabeth; Orwoll, Eric S

    2016-03-01

    The association between sex hormones and sex hormone binding globin (SHBG) with vertebral fractures in men is not well studied. In these analyses, we determined whether sex hormones and SHBG were associated with greater likelihood of vertebral fractures in a prospective cohort study of community dwelling older men. We included data from participants in MrOS who had been randomly selected for hormone measurement (N=1463, including 1054 with follow-up data 4.6years later). Major outcomes included prevalent vertebral fracture (semi-quantitative grade≥2, N=140, 9.6%) and new or worsening vertebral fracture (change in SQ grade≥1, N=55, 5.2%). Odds ratios per SD decrease in sex hormones and per SD increase in SHBG were estimated with logistic regression adjusted for potentially confounding factors, including age, bone mineral density, and other sex hormones. Higher SHBG was associated with a greater likelihood of prevalent vertebral fractures (OR: 1.38 per SD increase, 95% CI: 1.11, 1.72). Total estradiol analyzed as a continuous variable was not associated with prevalent vertebral fractures (OR per SD decrease: 0.86, 95% CI: 0.68 to 1.10). Men with total estradiol values ≤17pg/ml had a borderline higher likelihood of prevalent fracture than men with higher values (OR: 1.46, 95% CI: 0.99, 2.16). There was no association between total testosterone and prevalent fracture. In longitudinal analyses, SHBG (OR: 1.42 per SD increase, 95% CI: 1.03, 1.95) was associated with new or worsening vertebral fracture, but there was no association with total estradiol or total testosterone. In conclusion, higher SHBG (but not testosterone or estradiol) is an independent risk factor for vertebral fractures in older men.

  14. Divergence between growth hormone responses to insulin-induced hypoglycaemia and growth hormone-releasing hormone in patients with non-functioning pituitary macroadenomas and hyperprolactinaemia

    NARCIS (Netherlands)

    Beentjes, JAM; Sluiter, WJ; Dullaart, RPF

    1996-01-01

    OBJECTIVE The GH responses to the insulin tolerance test (ITT) and growth hormone-releasing hormone (GHRH) may yield different results in patients with pituitary lesions. The GH responses to these stimuli were compared in patients with untreated non-functioning pituitary macroadenomas, who represent

  15. Thyroid hormones and thyroid hormone receptors: Effects of thyromimetics on reverse cholesterol transport

    Institute of Scientific and Technical Information of China (English)

    Matteo; Pedrelli; Camilla; Pramfalk; Paolo; Parini

    2010-01-01

    Reverse cholesterol transport (RCT) is a complex process which transfers cholesterol from peripheral cells to the liver for subsequent elimination from the body via feces. Thyroid hormones (THs) affect growth, develop- ment, and metabolism in almost all tissues. THs exert their actions by binding to thyroid hormone receptors (TRs). There are two major subtypes of TRs, TRα and TRβ, and several isoforms (e.g. TRα1, TRα2, TRβ1, and TRβ2). Activation of TRα1 affects heart rate, whereas activation of TRβ1 has po...

  16. Analysis of human growth hormone gene 5' sequences in isolated growth hormone deficiency patients.

    OpenAIRE

    Wang, Y.; Yu, L L; Sheng, Q.; Meng, C; Sun, J.; S.S. Chen

    1994-01-01

    Human growth hormone (hGH) gene deletion (6.7 to 7.6 kb) is one of the causes of isolated growth hormone deficiency (IGHD), named IGHD IA. IGHD IA, however, only accounts for about 10% of the total IGHD patients. Most IGHD is caused by unknown mechanisms. Here, hGH gene 5' sequences in three IGHD patients without hGH gene deletion were analysed to see if there was any mutation hindering the expression of the hGH gene.

  17. Growth hormone, prolactin and thyrotrophin responses to thyrotrophin-releasing hormone in diabetic patients.

    OpenAIRE

    Harrower, A. D.

    1980-01-01

    Growth hormone (GH), prolactin (PRL) and thyrotrophin (TSH) responses to thyrotrophin-releasing hormone (TRH) were studied in 15 insulin-dependent diabetic patients. Basal plasma GH levels were raised above 5 mu./l in 6 patients and following the injection of TRH there was a significant rise in plasma GH levels in 9. The mean rise in plasma GH from basal to peak values was significant in the group as a whole (P < 0.01). Basal PRL and TSH levels were normal and rose normally in response to TRH...

  18. Effects of Growth Hormone Replacement Therapy on Bone Mineral Density in Growth Hormone Deficient Adults: A Meta-Analysis

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    Peng Xue

    2013-01-01

    Full Text Available Objectives. Growth hormone deficiency patients exhibited reduced bone mineral density compared with healthy controls, but previous researches demonstrated uncertainty about the effect of growth hormone replacement therapy on bone in growth hormone deficient adults. The aim of this study was to determine whether the growth hormone replacement therapy could elevate bone mineral density in growth hormone deficient adults. Methods. In this meta-analysis, searches of Medline, Embase, and The Cochrane Library were undertaken to identify studies in humans of the association between growth hormone treatment and bone mineral density in growth hormone deficient adults. Random effects model was used for this meta-analysis. Results. A total of 20 studies (including one outlier study with 936 subjects were included in our research. We detected significant overall association of growth hormone treatment with increased bone mineral density of spine, femoral neck, and total body, but some results of subgroup analyses were not consistent with the overall analyses. Conclusions. Our meta-analysis suggested that growth hormone replacement therapy could have beneficial influence on bone mineral density in growth hormone deficient adults, but, in some subject populations, the influence was not evident.

  19. The replacement of serum by hormones in cell culture media.

    Science.gov (United States)

    Sato, G; Hayashi, I

    1976-12-01

    The replacement of serum by hormones in cell culture media. (Reemplazo del suero por hormonas en el medio de cultivo de células). Arch. Biol. Med. Exper. 10: 120-121, 1976. The serum used in cell culture media can be replaced by a mixture of hormones and some accesory blood factors. The pituitary cell line GH3 can be grown in a medium in which serum is replaced by triiodothyronine, transferrin, parathormone, tyrotrophin releasing hormone and somatomedins. Hela and BHK cell strains can also be grown in serum free medium supplemented with hormones. Each cell type appears to have different hormonal requirements yet it may found that some hormones are required for most cell types.

  20. The replacement of serum by hormones in cell culture media.

    Science.gov (United States)

    Sato, G; Hayashi, I

    1976-12-01

    The replacement of serum by hormones in cell culture media. (Reemplazo del suero por hormonas en el medio de cultivo de células). Arch. Biol. Med. Exper. 10: 120-121, 1976. The serum used in cell culture media can be replaced by a mixture of hormones and some accesory blood factors. The pituitary cell line GH3 can be grown in a medium in which serum is replaced by triiodothyronine, transferrin, parathormone, tyrotrophin releasing hormone and somatomedins. Hela and BHK cell strains can also be grown in serum free medium supplemented with hormones. Each cell type appears to have different hormonal requirements yet it may found that some hormones are required for most cell types. PMID:1026199