WorldWideScience

Sample records for anti-human globulin anti-igg

  1. Effects of anti-human T lymphocyte immune globulins in patients: new or old.

    Science.gov (United States)

    Wang, Diane C; Wang, Xiangdong; Chen, Chengshui

    2016-09-01

    Multiple studies demonstrated that anti-human T lymphocyte immune globulins (ATG) can decrease the incidence of acute and chronic graft rejection in cell or organ transplants. However, further in-depth study indicates that different subgroups may benefit from either different regimes or alteration of them. Studies among renal transplant patients indicate that low immunological risk patients may not gain the same amount of benefit and thus tilt the risk versus benefit consideration. This may hold true for low immunological risk patients receiving other organ transplants and would be worth further investigation. The recovery time of T cells and natural killer (NK) cells also bears consideration and the impact that it has on the severity and incidence of opportunistic infections closely correlated with the dosage of ATG. The use of lower doses of ATG in combination with other induction medications may offer a solution. The finding that ATG may lose efficacy in cases of multiple transplants or re-transplants in the case of heart transplants may hold true for other transplantations. This may lead to reconsideration of which induction therapies would be most beneficial in the clinical setting. These studies on ATG done on different patient groups will naturally not be applicable to all, but the evidence accrued from them as a whole may offer us new and different perspectives on how to approach and potentially solve the clinical question of how to best reduce the mortality associated with chronic host-versus-graft disease. PMID:27084794

  2. 猪抗淋巴细胞免疫球蛋白联合环孢素治疗重型再生障碍性贫血疗效观察%Therapeutic effect of porcine anti- human lymphocyte globulin combined with cyclosporine A therapy on severe aplastic anemia

    Institute of Scientific and Technical Information of China (English)

    王春生

    2012-01-01

    目的 探讨猪抗淋巴细胞免疫球蛋白与环孢素联合应用于治疗重型再生障碍性贫血(再障)的临床疗效及临床转归.方法 50例重型再障患者作为研究资料,治疗组给予猪抗淋巴细胞免疫球蛋白与环孢素联合治疗,对照组给予猪抗淋巴细胞免疫球蛋白治疗,观察并对比两组患者疗效、起效时间以及不良反应.结果 治疗组总有效率明显高于治疗组,基本治愈率、明显缓解率以及有所改善率均高于对照组.经过治疗后两组患者白细胞(WBC)、血红蛋白(HGB)以及血小板(PLT)均明显升高,但治疗后治疗组WBC、HGB以及PLT均明显高于对照组.治疗组平均起效时间、牙龈增生出现例数明显高于对照组,另出现5例手颤,3例继发性糖尿病,而对照组未发现此类不良反应.结论 猪抗淋巴细胞免疫球蛋白与环孢素联合应用于治疗重型再障疗效优于单用猪抗淋巴细胞免疫球蛋白治疗,但不良反应较单用猪抗淋巴细胞免疫球蛋白治疗更多,可以推荐应用,如何防治并发症仍值得思考.%Objective To investigate the clinical efficacy of porcine anti -human lymphocyte globulin combined with cyclosporine A in treatment of severe aplastic anemia (SAA). Methods Fifty SAA patients were included in the study. The treatment group received the porcine anti -human lymphocyte globulin combined with cyclosporine A therapy, while the control group received only porcine anti - human lymphocyte globulin therapy. The efficacy, onset time, and adverse events were observed and compared between the two groups. Results The overall efficacy rate, basic cure rate, and apparent remission rate were significantly higher in the treatment group than in the control group. After treatment, white blood cells (WBC), hemoglobin (HGB), and platelets (PLT) were significantly increased in both groups, but significantly higher in the treatment group than in the control group. The average onset time and

  3. SHBG (Sex Hormone Binding Globulin)

    Science.gov (United States)

    ... as: Testosterone-estrogen Binding Globulin; TeBG Formal name: Sex Hormone Binding Globulin Related tests: Testosterone , Free Testosterone, ... I should know? How is it used? The sex hormone binding globulin (SHBG) test may be used ...

  4. Serum globulines as radioprotective substances

    International Nuclear Information System (INIS)

    It is established on the basis of literary and experimental data that the preparations of globulin increase the survivability of irradiated animals and contribute to the normalization of the protective function of the immune system

  5. Sex hormone binding globulin phenotypes

    DEFF Research Database (Denmark)

    Cornelisse, M M; Bennett, Patrick; Christiansen, M;

    1994-01-01

    Human sex hormone binding globulin (SHBG) is encoded by a normal and a variant allele. The resulting SHBG phenotypes (the homozygous normal SHBG, the heterozygous SHBG and the homozygous variant SHBG phenotype) can be distinguished by their electrophoretic patterns. We developed a novel detection...

  6. Purification of equine Gc-globulin

    DEFF Research Database (Denmark)

    Houen, Gunnar; Pihl, Tina Holberg; Andersen, Pia Haubro;

    to be a sensitive marker of acute tissue injury and fatal outcome in humans. Patients with a low plasma concentration of Gc-globulin due to severe tissue injury might potentially benefit from infusions with purified Gc-globulin [1]. With an equine Gc-globulin assay, future studies will investigate the concentration...... of Gc-globulin in colic horses with intestinal ischemia were Gc-globulin might be useful as a diagnostic and prognostic marker. Horses with intestinal ischemia often die, despite of expensive surgical treatment, because of endotoxemia and shock, therefore these horses potentially could benefit from Gc......-globulin infusions. Reference List    1.   Vasconcellos CA and Lind SE. Coordinated inhibition of actin-induced platelet aggregation by plasma gelsolin and vitamin D-binding protein. Blood 1993;82:3648-3657....

  7. Profile of total protein, albumin, globulin and albumin globulin ratio in bulls

    OpenAIRE

    Ida Zahidah Irfan; Esfandiari, A; C Choliq

    2014-01-01

    Determination of serum total protein concentration and main fractions (albumin and globulin) can be used as an important diagnostic tool in clinical biochemistry. Several factors can affect the concentration of total protein, albumin, globulin and albumin globulin ratio (A/G). The aim of this study is to obtain serum protein profiles, albumin, globulin and A/G ratio based on breed, age and BCS (body condition score). Blood samples from 160 bulls were collected. Blood chemistry were analyzed ...

  8. Total Protein and Albumin/Globulin Ratio Test

    Science.gov (United States)

    ... limited. Home Visit Global Sites Search Help? Total Protein and Albumin/Globulin (A/G) Ratio Share this ... Globulin Ratio; A/G Ratio Formal name: Total Protein; Albumin to Globulin Ratio Related tests: Albumin ; Liver ...

  9. Profile of total protein, albumin, globulin and albumin globulin ratio in bulls

    Directory of Open Access Journals (Sweden)

    Ida Zahidah Irfan

    2014-06-01

    Full Text Available Determination of serum total protein concentration and main fractions (albumin and globulin can be used as an important diagnostic tool in clinical biochemistry. Several factors can affect the concentration of total protein, albumin, globulin and albumin globulin ratio (A/G. The aim of this study is to obtain serum protein profiles, albumin, globulin and A/G ratio based on breed, age and BCS (body condition score. Blood samples from 160 bulls were collected. Blood chemistry were analyzed by photometer principle using a commercial kit. There were significant (P<0.001 breed variation on total protein, albumin, globulin and albumin globulin ratio. Significant age differences were observed on total protein and albumin concentration (P<0.001, while globulin concentration and A/G ratio were also significant (P<0.05. Amongs groups of BCS, significant difference was verified only in the albumin concentration (P<0.05. The concentration of total proteins, albumins and globulins in the serum of the bulls are higher than standard values for cattle, while A/G ratio is lower.

  10. Gc globulin as a diagnostic and prognostic marker in horses

    DEFF Research Database (Denmark)

    Pihl, Tina Holberg

    can prevent development of shock and thereby increase survival chances. The in vivo toxicity of Gc-globulin infusion is currently being investigated in horses and other species. Gc-globulin has been demonstrated in horse plasma and its structure closely resembles that of human Gc-globulin. Gc...

  11. Trial of Immune Globulin in Infant Botulism

    Directory of Open Access Journals (Sweden)

    J Gordon Millichap

    2006-02-01

    Full Text Available A 5-year, randomized, double-blind, placebo-controlled trial of the orphan drug Human Botulism Immune Globulin Intravenous (BIG-IV in 122 infants in California with confirmed infant botulism (75 caused by type A Clostridium botulinum toxin, and 47 by type B toxin was conducted at the California Department of Health Services, Richmond, CA; National Botulism Surveillance and Reference Laboratory, CDC and P, Atlanta; and Division of Biostatistics, University of California, Berkeley.

  12. Purified equine rabies immune globulin: a safe and affordable alternative to human rabies immune globulin.

    OpenAIRE

    Wilde, H. De; Chomchey, P.; Punyaratabandhu, P; Phanupak, P.; Chutivongse, S.

    1989-01-01

    Reported are the results of a retrospective study of 3156 patients who were treated at the Queen Saovabha Memorial Institute, Bangkok, with equine rabies immune globulin (ERIG). Only 51 patients (1.6%) exhibited serum-sickness-like reactions, none of which persisted for more than a week, and only 8 of these patients (15%) were treated with a short course of steroids. One patient, whose skin test was negative, had an immediate anaphylactic reaction to ERIG that responded to parenteral therapy ...

  13. 21 CFR 866.5400 - Alpha-globulin immuno-logical test system.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Alpha-globulin immuno-logical test system. 866.5400 Section 866.5400 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN....5400 Alpha-globulin immuno-logical test system. (a) Identification. An alpha-globulin...

  14. Treatment of neonatal sepsis with intravenous immune globulin

    DEFF Research Database (Denmark)

    Brocklehurst, Peter; Farrell, Barbara; King, Andrew;

    2011-01-01

    Neonatal sepsis is a major cause of death and complications despite antibiotic treatment. Effective adjunctive treatments are needed. Newborn infants are relatively deficient in endogenous immunoglobulin. Meta-analyses of trials of intravenous immune globulin for suspected or proven neonatal sepsis...

  15. 21 CFR 862.1330 - Globulin test system.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Globulin test system. 862.1330 Section 862.1330 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES CLINICAL CHEMISTRY AND CLINICAL TOXICOLOGY DEVICES Clinical Chemistry Test Systems §...

  16. ALTERATIONS IN TOTAL PROTEIN CONCENTRATION, SERUM PROTEIN FRACTIONS AND ALBUMIN/GLOBULIN RATIO IN HEALTHY RABBITS

    OpenAIRE

    Nuzhat Sultana; Rahila Najam

    2013-01-01

    This study assessed the effect of oral administration of Aloe vera and was to evaluate total serum protein, albumin and globulin concentrations as well as albumin / globulin (A / G) ratio. Twenty rabbits weighing 1000 – 1800 g were divided into 2 groups. Each group consisted of ten animals. One served as control and other group served as experimental group. Results show that animals after 07, 15 and 30 days dosing of Aloe vera showed highly significant decrease in total protein and globulin a...

  17. Sex hormone binding globulin in the rat olfactory system.

    Science.gov (United States)

    Ploss, V; Gebhart, V M; Dölz, W; Jirikowski, G F

    2014-05-01

    Ovarian steroids are known to act on the olfactory system. Their mode of action, however, is mostly unclear to date since nuclear receptors are lacking in sensory neurons. Here we used immunocytochemistry and RT-PCR to study expression and distribution of sex hormone binding globulin (SHBG) in the rat olfactory system. Single sensory cells in the olfactory mucosa and their projections in the olfactory bulb showed specific SHBG immunostaining as determined by double immunofluorescence with olfactory marker protein OMP. Larger groups of SHBG stained sensory cells occurred in the vomeronasal organ (VNO). A portion of the olfactory glomeruli in the accessory olfactory bulb showed large networks of SHBG positive nerve fibres. Some of the mitral cells showed SHBG immune fluorescence. RT-PCR revealed SHBG encoding mRNA in the olfactory mucosa, in the VNO and in the olfactory bulbs indicating intrinsic expression of the binding globulin. The VNO and its related projections within the limbic system are known to be sensitive to gonadal steroid hormones. We conclude that SHBG may be of functional importance for rapid effects of olfactory steroids on limbic functions including the control of reproductive behaviours through pheromones. PMID:24681170

  18. Strain-specific renal toxicity of heterologous antilymphocyte [gamma]-globulin in mice7

    NARCIS (Netherlands)

    Cohen, B.J.; Vries, M.J. de; Noord, M.J. van; Lubbe, F.H.

    1970-01-01

    Severe glomerulonephritis followed one to five weekly i.p. injections in TLFM mice of rabbit antimouse lymphocyte [gamma]-globulin (ALG). Glomerulonephritis did not occur in C57BL mice subjected to the same regimen. Administration of normal rabbit [gamma]-globulin (NRG) to RFM mice also caused renal

  19. Screening of Fungi from Chinese Medical Plants for Anti-Human Immunodeficiency Virus Type 1 Activity

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    In order to isolate anti-human immunodeficiency virus (HIV) agents from natural products, 97 ethanolic extracts of 90 fungi were tested for their inhibitory activity on HIV-1. Most of the extracts tested were relatively non-toxic to human lymphocytic MT-4 cells, but extracts of some fungi exhibited potent anti-HIV activity in an in vitro 3-(4,5-dimethyl-2 thiazoyl)-2,5-diphenyl-2H-tetrazolium bromide assay with a selectivity index greater than 3. Most fungi were isolated from Dendrobium sp. and Taxus sp.

  20. SIFAT FUNGSIONAL PRODUK INTERAKSI FRAKSI GLOBULIN 7S KOMAK (Dolichos lablab DAN GUM XANTAN [Functional Properties of the Interaction Product Between Globulin of 7S Fraction of Lablab Bean (Dolichos lablab with Xantan Gum

    Directory of Open Access Journals (Sweden)

    Sukamto1*

    2009-12-01

    Full Text Available Lablab bean (Dolichos lablab seeds is a potential source of protein globulin.The bean’s protein content is 20.86 %, and the amount of globulin was more than 60% from the total protein, having major fractions of 7S and 11S. The objectives of this research were to explore the 7S globulin fractions, to study interaction between 7S globulin fractions with xanthan gum, and to observe the functional properties of the product of the interaction. The research was conducted in 2 steps. The first step was to fractionate the 7S fractions from globulin. The second steps was to interact 7S globulin fraction with xanthan gum. The yield of these interaction were examined for its physicochemical and functional properties. The results showed that the 7S globulin fractions could be interacted by xanthan gum at pH 7. The interacted product of globulin 7S fraction 10 % with xanthan gum 0,75 % had good functional properties than globulin 7S fraction, such as oil holding capacity, foaming capacity, and emulsion activity. Water holding capacity could not be detected because the yield became soluble. However,the foaming and emulsifying stability were still lower than those of soybean protein isolates. The research concluded that xanthan gum could be used to improve the physicochemical and functional properties of globulin 7S fraction.

  1. Treatment of Severe Aplastic Anemia by Immunosuppressor Anti-lymphocyte Globulin/Anti-thymus Globulin as the Chief Medicine in Combination with Chinese Drugs

    Institute of Scientific and Technical Information of China (English)

    郑兵荣; 沈建平; 庄海峰; 林圣云; 沈一平; 周郁鸿

    2009-01-01

    Objective:To study the therapeutic effect of combined therapy with Chinese drugs and immunosuppressors, mainly anti-lymphocyte globulin/anti-thymus globulin(ALG/ATG),for the treatment of severe aplastic anemia(SAA),the efficacy associated factors and adverse effects as well.Methods:A retrospective analysis was conducted on 65 patients with SAA treated by combined therapy which was supplemented with cyclosporin A,androgen,hematopoietic growth factor,etc.Results:Of the 57 patients followed-up,26 (45.6%) we...

  2. Expression of corticosteroid binding globulin in the rat olfactory system.

    Science.gov (United States)

    Dölz, Wilfried; Eitner, Annett; Caldwell, Jack D; Jirikowski, Gustav F

    2013-05-01

    Glucocorticoids are known to act on the olfactory system although their mode of action is still unclear since nuclear glucocorticoid receptors are mostly absent in the olfactory mucosa. In this study we used immunocytochemistry, in situ hybridization, and RT-PCR to study the expression and distribution of corticosteroid binding globulin (CBG) in the rat olfactory system. Mucosal goblet cells could be immunostained for CBG. Nasal secretion contained measurable amounts of CBG suggesting that CBG is liberated. CBG immunoreactivity was localized in many of the basal cells of the olfactory mucosa, while mature sensory cells contained CBG only in processes as determined by double immunostaining with the olfactory marker protein OMP. This staining was most pronounced in the vomeronasal organ (VNO). The appearance of CBG in the non-sensory and sensory parts of the VNO and in nerve terminals in the accessory bulb indicated axonal transport. Portions of the periglomerular cells, the mitral cells and the tufted cells were also CBG positive. CBG encoding transcripts were confirmed by RT-PCR in homogenates of the olfactory mucosa and VNO. Olfactory CBG may be significant for uptake, accumulation and transport of glucocorticoids, including aerosolic cortisol. PMID:23141917

  3. Thermal denaturation of sunflower globulins in low moisture conditions

    International Nuclear Information System (INIS)

    DSC analysis in pressure resisting pans of sunflower oil cake makes appear the endothermic peak of sunflower globulins denaturation. Its temperature decreases from 189.5 to 119.9 deg. C while the corresponding enthalpy increases from 2.6 to 3.3 J/g of sample, or from 6.7 to 12.2 J/g of dry protein, when the samples moisture content varies from 0 to 30.0% of the total weight. The plot of the denaturation temperature versus the moisture content is not linear but has a rounded global shape and seems to follow the hydration behavior of the proteins, modeled with the sorption isotherm. As it can be seen on scanning electron microscopy (SEM) micrographs, protein corpuscles 'melt' after such a thermal treatment and large aggregates form by coagulation. Moisture dependence of the 'fusion' temperature of native proteic organization, in low moisture conditions, offers so a new characterization method for the use of vegetable proteins in agro-materials

  4. Grizzly bear corticosteroid binding globulin: Cloning and serum protein expression.

    Science.gov (United States)

    Chow, Brian A; Hamilton, Jason; Alsop, Derek; Cattet, Marc R L; Stenhouse, Gordon; Vijayan, Mathilakath M

    2010-06-01

    Serum corticosteroid levels are routinely measured as markers of stress in wild animals. However, corticosteroid levels rise rapidly in response to the acute stress of capture and restraint for sampling, limiting its use as an indicator of chronic stress. We hypothesized that serum corticosteroid binding globulin (CBG), the primary transport protein for corticosteroids in circulation, may be a better marker of the stress status prior to capture in grizzly bears (Ursus arctos). To test this, a full-length CBG cDNA was cloned and sequenced from grizzly bear testis and polyclonal antibodies were generated for detection of this protein in bear sera. The deduced nucleotide and protein sequences were 1218 bp and 405 amino acids, respectively. Multiple sequence alignments showed that grizzly bear CBG (gbCBG) was 90% and 83% identical to the dog CBG nucleotide and amino acid sequences, respectively. The affinity purified rabbit gbCBG antiserum detected grizzly bear but not human CBG. There were no sex differences in serum total cortisol concentration, while CBG expression was significantly higher in adult females compared to males. Serum cortisol levels were significantly higher in bears captured by leg-hold snare compared to those captured by remote drug delivery from helicopter. However, serum CBG expression between these two groups did not differ significantly. Overall, serum CBG levels may be a better marker of chronic stress, especially because this protein is not modulated by the stress of capture and restraint in grizzly bears.

  5. Safety Pharmacology, Toxicology and Pharmacokinetic Assessment of Human Gc Globulin (Vitamin D Binding Protein)

    DEFF Research Database (Denmark)

    Pihl, Tina Holberg; Jørgensen, Charlotte Svaerke; Santoni-Rugiu, Eric;

    2010-01-01

    Gc globulin is an important protein of the plasma actin-scavenger system. As such, it has been shown to bind free actin and prevent hypercoagulation and shock in patients with massive actin release resulting from severe tissue injuries. Treatment of such patients with Gc globulin could therefore ....... The half-life, T, for human Gc globulin was 12 hr in rats, 16 hr in horses and 30 hr in dogs. The safety profile of plasma-derived Gc globulin is concluded to be consistent to that required for use in man....... potentially be life-saving. This article presents pre-clinical toxicology experiments conducted on purified plasma-derived human Gc globulin. The Gc globulin formulation was shown to be stable for at least 4 years with full retention of actin-binding capacity. In vitro studies did not reveal activation...... of the kallikrein system or the complement system and cellular studies showed no toxic effects on a variety of human cell lines. In vivo studies showed no acute toxic effects in mice, rats or guinea pigs upon intravenous infusion. A 14-day local tolerance study in rabbits showed no adverse effects, and 14-day...

  6. Safety pharmacology, toxicology and pharmacokinetic assesment of human Gc globulin (vitamin d binding protein)

    DEFF Research Database (Denmark)

    Pihl, Tina Holberg; Jørgensen, Charlotte Sværke; Santoni Rugiu, Eric;

    2010-01-01

      Gc globulin is an important protein of the plasma actin-scavenger system. As such, it has been shown to bind free actin and prevent hypercoagulation and shock in patients with massive actin release resulting from severe tissue injuries. Treatment of such patients with Gc globulin could therefor....... The half-life, T, for human Gc globulin was 12 hr in rats, 16 hr in horses and 30 hr in dogs. The safety profile of plasma-derived Gc globulin is concluded to be consistent to that required for use in man....... potentially be life-saving. This article presents pre-clinical toxicology experiments conducted on purified plasma-derived human Gc globulin. The Gc globulin formulation was shown to be stable for at least 4 years with full retention of actin-binding capacity. In vitro studies did not reveal activation...... of the kallikrein system or the complement system and cellular studies showed no toxic effects on a variety of human cell lines. In vivo studies showed no acute toxic effects in mice, rats or guinea pigs upon intravenous infusion. A 14-day local tolerance study in rabbits showed no adverse effects, and 14-day...

  7. Study of the influence of homologous serum globulin preparations on the intestinal automicroflora in irradiated mice

    Energy Technology Data Exchange (ETDEWEB)

    Pinegin, B.V.; Klemparskaya, N.N.; Mal' tsev, V.N.; Korshunov, G.A.; Shal' nova, G.A.; Kuz' mina, T.D.

    1984-09-01

    In spite of considerable experience of practical use of serum globulin preparations, their effect on automicroflora wasn't studied. The favorable effect of therapeutic injection of homologous serum globulin preparations on automicroflora of small and large intestine of mices was established for the model of acute radiation sickness caused by /sup 60/Co irradiation with 700 R dose. The effect of injecting two types of globulin preparations was studied: ones prepared of blood of intact and hemostimulated mices (to increase the content of normal antitissue antibodies in the serum). Besides the general globulin fraction isolated by ammonium sulfate precipitation a study was made on the effect of purified IgG and IgM preparations. Threefold subcutaneous or intraperitoneal globulin in ection of 1 ..mu..g dose in a mice prevented after 2, 24, 48 h after irradiation the development of bacteriosis, typical for radiation injury - decreased accumulation of putrefactive bacteria and reduced the suppression of lactobacilli content. Globulin preparations and fractions of hemostimulated mice serum, enriched by normal antitissue antibodies are the most effective ones.

  8. Safety pharmacology, toxicology and pharmacokinetic assessment of human Gc globulin (vitamin D binding protein).

    Science.gov (United States)

    Pihl, Tina Holberg; Jørgensen, Charlotte Svaerke; Santoni-Rugiu, Eric; Leifsson, Páll Skúli; Hansen, Erik Wind; Laursen, Inga; Houen, Gunnar

    2010-11-01

    Gc globulin is an important protein of the plasma actin-scavenger system. As such, it has been shown to bind free actin and prevent hypercoagulation and shock in patients with massive actin release resulting from severe tissue injuries. Treatment of such patients with Gc globulin could therefore potentially be life-saving. This article presents pre-clinical toxicology experiments conducted on purified plasma-derived human Gc globulin. The Gc globulin formulation was shown to be stable for at least 4 years with full retention of actin-binding capacity. In vitro studies did not reveal activation of the kallikrein system or the complement system and cellular studies showed no toxic effects on a variety of human cell lines. In vivo studies showed no acute toxic effects in mice, rats or guinea pigs upon intravenous infusion. A 14-day local tolerance study in rabbits showed no adverse effects, and 14-day toxicity studies in rats and horses did not show any unwanted reactions. In a 14-day toxicology study in beagle dogs, formation of antibodies was seen and in the end of the study period, three out of four dogs showed clinical immunological reactions, which could be ascribed to the formation of antibodies. The half-life, T, for human Gc globulin was 12 hr in rats, 16 hr in horses and 30 hr in dogs. The safety profile of plasma-derived Gc globulin is concluded to be consistent to that required for use in man. PMID:20560927

  9. Effect of Co-60 irradiation on hyperimmune antimeningococcus globulins-gamma

    International Nuclear Information System (INIS)

    Globulins-gamma from voluntary blood donors immunized with the Cuban BC antimeningococcus vaccine is now being used in our country for the treatment of the meningococcus disease. This study of the effect of Co-60 irradiation on antimeningococcus globulins-gamma was carried out to try to eliminate the inconvenience shown by the traditionally used sterilization procedures (losses in the filter and persistence of viral contamination). globulins-gamma was obtained by ethanol fractionation and was irradiated at a different dose in solution with different stabilizers and it was also lyophilized. Results of the chemical controls carried out lead to the conclusion that it is possible to use radiosterilization on this product in a lyophilized form. The preservation of bactericidal activity, even after the highest irradiation doses, confirms the above mentioned. 13 refs

  10. ALTERATIONS IN TOTAL PROTEIN CONCENTRATION, SERUM PROTEIN FRACTIONS AND ALBUMIN/GLOBULIN RATIO IN HEALTHY RABBITS

    Directory of Open Access Journals (Sweden)

    Nuzhat Sultana

    2013-08-01

    Full Text Available This study assessed the effect of oral administration of Aloe vera and was to evaluate total serum protein, albumin and globulin concentrations as well as albumin / globulin (A / G ratio. Twenty rabbits weighing 1000 – 1800 g were divided into 2 groups. Each group consisted of ten animals. One served as control and other group served as experimental group. Results show that animals after 07, 15 and 30 days dosing of Aloe vera showed highly significant decrease in total protein and globulin and highly significant decrease in Albumin after 15 and 30 days of dosing of Aloe vera in comparison to control animals group. It is concluded that the long-term use of Aloe vera may cause hypoglobinemia and hypoalbuminemia at 30 days of dosing and it could be due to the liver diseases, evidence of hepatotoxicity induced Aloe vera also reported in previous studies.

  11. Comparison between IV immune globulin (IVIG) and anti-D globulin for treatment of immune thrombocytopenia: a randomized open-label study.

    Science.gov (United States)

    Eghbali, Aziz; Azadmanesh, Peyman; Bagheri, Bahador; Taherahmadi, Hasan; Sadeghi Sedeh, Bahman

    2016-08-01

    To compare the effect of IV immune globulin (IVIG) and anti-D globulin (anti-D) for treatment of immune thrombocytopenia (ITP) in children. A randomized, open-label, single-center clinical trial was carried out in Amir-Kabir Hospital (Arak, Iran). The study was performed on 60 children with acute and chronic ITP, aged from 1 to 15 years. Patients were randomly assigned (1:1) to 50 μg/kg anti-D or 1 g/kg IVIG. Platelet counting was performed at baseline and at 3, 7, and 14 days after treatment termination. Safety assessment was performed in all patients. Anti-D caused a quicker response on the 3rd day of treatment (P anti-D had lower rate of side effects including fever (P anti-D was associated with rapid rise of platelets compared to IVIG. In addition, anti-D treatment had acceptable safety profile. PMID:26991138

  12. Effect of High Temperature on Albumin and Globulin Accumulation in the Endosperm Proteome of the Developing Wheat Grain

    Science.gov (United States)

    The effect of high temperature during grain fill on albumin and globulin accumulation profiles was investigated in the endosperm of developing wheat (Triticum aestivum, L. cv. Butte 86) grain. Albumins and globulins were isolated from endosperm of grain grown under a moderate (24°C/17°C, day/night) ...

  13. Prognostic Value of Gc-Globulin in Chinese Patients with Acute-On-Chronic Hepatitis B Liver Failure

    International Nuclear Information System (INIS)

    Objective: To determine dynamic Gc-globulin level change in Acute-on-Chronic Hepatitis B Liver Failure (ACHBLF) patients, and evaluate the prognostic value of Gc-globulin. Study Design: An analytical study. Place and Duration of Study: The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China, from January 2010 to December 2012. Methodology: A total of 54 consecutive Chinese ACHBLF patients and 30 healthy volunteers as controls were recruited from 2010 to 2012. The patients were divided into improved group and aggravated group. Gc-globulin levels were determined in both groups and mean values compared with significance at p < 0.05. Cut-off value was also determined. Results: The Gc-globulin level was significantly decreased in ACHBLF patients (p < 0.001). Gc-globulin levels were significantly higher in improved patients than in aggravated patients, and a 215 mg/L cut-off value carried the best prognostic information. On longitudinal observations, Gc-globulin gradually elevated in improved groups. However, in aggravated groups, the Gc-globulin levels were always below normal levels and no significant change was observed before or after the treatment (p > 0.05). Conclusion: Gc-globulin monitoring offers a rapid and accurate method to estimate treatment outcomes on admission and an effective temporal indicator of curative effects in ACHBLF patients at an optimal cut-off value of 215 mg/L. (author)

  14. Sex hormone-binding globulin levels predict insulin sensitivity, disposition index, and cardiovascular risk during puberty

    DEFF Research Database (Denmark)

    Sørensen, Kaspar; Aksglaede, Lise; Munch-Andersen, Thor;

    2009-01-01

    Early puberty is associated with increased risk of subsequent cardiovascular disease. Low sex hormone-binding globulin (SHBG) levels are a feature of early puberty and of conditions associated with increased cardiovascular risk. The aim of the present study was to evaluate SHBG as a predictor of ...

  15. Low level of serum sex hormone binding globulin is associated with the occurrence of metabolic syndrome

    Institute of Scientific and Technical Information of China (English)

    缪琳

    2013-01-01

    Objective To investigate the relationship between serum sex hormone binding globulin (SHBG) and metabolic syndrome (MS) in Chinese young population.Methods A total of 797 patients were enrolled and subdivided into MS group (n=377) and non-MS group (n=420) .Body height and weight were measured for body

  16. Increased sex hormone-binding globulin levels in children and adolescents with thyrotoxicosis

    DEFF Research Database (Denmark)

    Nielsen, J; Jensen, Rikke Bodin Beck; Juul, Anders

    2013-01-01

    Thyrotoxicosis is a rare condition in pediatric patients, and optimal treatment can be difficult to achieve in some children. To our knowledge, no studies have evaluated sex hormone-binding globulin (SHBG) levels in hyperthyroid children and adolescents in relation to age- and gender...

  17. Passive immune globulin therapy in the SIV/macaque model : Early intervention can alter disease profile

    NARCIS (Netherlands)

    Haigwood, NL; Watson, A; Sutton, WF; McClure, J; Lewis, A; Ranchalis, J; Travis, B; Voss, G; Letvin, NL; Hu, SL; Hirsch, VM; Johnson, PR

    1996-01-01

    One of the major questions in AIDS is the role that the host immune system and the virus play in the dynamics of infection and the development of AIDS in an infected individual. In order to test the role of antibody in controlling viral infection, high-dose SIV-immune globulin was passively transfer

  18. 21 CFR 862.1685 - Thyroxine-binding globulin test system.

    Science.gov (United States)

    2010-04-01

    ... protein which binds thyroxine, in serum and plasma. Measurements obtained by this device are used in the... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Thyroxine-binding globulin test system. 862.1685 Section 862.1685 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN...

  19. Modification of solubility and heat-induced gelation of amaranth 11S globulin by protein engineering.

    Science.gov (United States)

    Carrazco-Peña, Laura; Osuna-Castro, Juan A; De León-Rodríguez, Antonio; Maruyama, Nobuyuki; Toro-Vazquez, Jorge F; Morales-Rueda, Juan A; Barba de la Rosa, Ana P

    2013-04-10

    The primary structure of amaranth 11S globulin (Ah11S) was engineered with the aim to improve its functional properties. Four continuous methionines were inserted in variable region V, obtaining the Ah11Sr+4M construction. Changes on protein structure and surface characteristics were analyzed in silico. Solubility and heat-induced gelation of recombinant amaranth 11S proglobulin (Ah11Sr and Ah11Sr+4M) were compared with the native protein (Ah11Sn) purified from amaranth seed flour. The Ah11Sr+4 M showed the highest surface hydrophobicity, but as consequence the solubility was reduced. At low ionic strength (μ = 0.2) and acidic pH (proteins Ah11Sr and Ah11Sr+4 M had the highest and lowest solubility values, respectively. All globulins samples formed gels at 90 °C and low ionic strength, but Ah11Sn produced the weakest and Ah11Sr the strongest gels. Differential scanning calorimetry analysis under gel forming conditions revealed only exothermic transitions for all amaranth 11S globulins analyzed. In conclusion, the 3D structure analysis has revealed interesting molecular features that could explain the thermal resistance and gel forming ability of amaranth 11S globulins. The incorporation of four continuous methionines in amaranth increased the hydrophobicity, and self-supporting gels formed had intermediate hardness between Ah11Sn and Ah11Sr. These functional properties could be used in the food industry for the development of new products based on amaranth proteins.

  20. Is the sex hormone binding globulin related to preeclampsia independent of insulin resistance

    International Nuclear Information System (INIS)

    Objective: To evaluate the association between Sex Hormone Binding Globulin and preeclampsia in Iranian women considering the probable confounding effect of insulin resistance. Methods: The case-control study was conducted at the Semnan University of Medical Sciences, Iran, and comprised pregnant women who received prenatal care at Amiralmomenin Hospital in 2011. Cases represented patients admitted because of preeclampsia, while controls were randomly selected eligible pregnant women without hypertension and/or proteinuria. Fasting blood sugar and insulin were assessed for all participants as well as their blood concentration of Sex Hormone Binding Globulin. The Homeostasis Model Assessment of Insulin Resistance Score was used. The correlation between dependant and independent variables was reported by crude and adjusted odds ratio applying logistic regression models. SPSS 16.0 was used for statistical analysis. Results: Of the 100 pregnant women in the study, 45(45%) were cases. Insulin resistance was found to be significantly more frequent in the cases compared to the controls (adjusted odds ratio=2.78; 95% Confidence Interval: 1.11, 6.90; p<0.01). There was a significant reverse correlation between level of Sex Hormone Binding Globulin in blood and being a case of preeclampsia (adjusted odds ratio=0.99; 95% Confidence Interval: 0.98, 1.00; p=0.04). Conclusion: Independent of insulin resistance, every 1nmol/l increase in Sex Hormone Binding Globulin, decreases the odds of preeclampsia by 1%, notifying Sex Hormone Binding Globulin as an important biomarker about its etiology and prediction. (author)

  1. Identification of three wheat globulin genes by screening a Triticum aestivum BAC genomic library with cDNA from a diabetes-associated globulin

    Directory of Open Access Journals (Sweden)

    MacFarlane Amanda J

    2009-07-01

    Full Text Available Abstract Background Exposure to dietary wheat proteins in genetically susceptible individuals has been associated with increased risk for the development of Type 1 diabetes (T1D. Recently, a wheat protein encoded by cDNA WP5212 has been shown to be antigenic in mice, rats and humans with autoimmune T1D. To investigate the genomic origin of the identified wheat protein cDNA, a hexaploid wheat genomic library from Glenlea cultivar was screened. Results Three unique wheat globulin genes, Glo-3A, Glo3-B and Glo-3C, were identified. We describe the genomic structure of these genes and their expression pattern in wheat seeds. The Glo-3A gene shared 99% identity with the cDNA of WP5212 at the nucleotide and deduced amino acid level, indicating that we have identified the gene(s encoding wheat protein WP5212. Southern analysis revealed the presence of multiple copies of Glo-3-like sequences in all wheat samples, including hexaploid, tetraploid and diploid species wheat seed. Aleurone and embryo tissue specificity of WP5212 gene expression, suggested by promoter region analysis, which demonstrated an absence of endosperm specific cis elements, was confirmed by immunofluorescence microscopy using anti-WP5212 antibodies. Conclusion Taken together, the results indicate that a diverse group of globulins exists in wheat, some of which could be associated with the pathogenesis of T1D in some susceptible individuals. These data expand our knowledge of specific wheat globulins and will enable further elucidation of their role in wheat biology and human health.

  2. Cross-reactivity of anti-human cytokine monoclonal antibodies used as a tool to identify novel immunological biomarkers in domestic ruminants.

    Science.gov (United States)

    Dorneles, E M S; Araújo, M S S; Teixeira-Carvalho, A; Martins-Filho, O A; Lage, A P

    2015-01-01

    Eleven commercially available PE-labeled anti-human (IL-1-α, IL-6, IL-8, TNF-α, IL-17A, IL-5, IL-10, IL-12 and IL-13) and anti-mouse (IL-10, TNF-α) cytokine monoclonal antibodies (mAbs) were tested for cross-reactivity with cattle, goat, and sheep cytokines. Cross-reactivity was assessed by comparative analysis with the standard reactivity of the target species. Our data demonstrated that anti-human IL-1-α, IL-6, IL-8, IL-17A and IL-10 mAbs cross-react with all ruminant species tested. Anti-human IL-5 mAb showed a strong cross-reactivity with cattle and goat IL-5, while anti-human TNF-α mAb showed a selective cross-reactivity with goat TNF-α. No cross-reactivity with the ruminant cytokines was observed for anti-human IL-12 and IL-13 mAbs or for the two anti-mouse cytokine mAbs tested. The present study demonstrated the cross-reactivity of various anti-human cytokine mAbs with cattle, sheep, and goat cytokines, increasing the range of immunological biomarkers for studies in veterinary medicine. PMID:25730032

  3. Immunomodulation with rabbit anti-thymocyte globulin in solid organ transplantation.

    Science.gov (United States)

    Ippoliti, Giovanbattista; Lucioni, Marco; Leonardi, Giuseppe; Paulli, Marco

    2015-12-24

    Rabbit anti-thymocyte globulin's manifold mechanisms of action may be attribuited to its polyclonal nature. Its T-cell depleting effect on lymphoid cells is well established: Occurring in the blood and secondary lymphoid tissues, depletion proceeds through complement-dependent lysis, opsonization and apoptotic pathways. Clinical studies have shown that rabbit anti-thymocyte globulin's immunomodulatory effect extends beyond the initial T-cell depletion and up to the period during which lymphocyte populations begin to recover. The drug is able to mediate immunomodulation and graft tolerance by functionally inactivating cell surface receptors involved in antigen recognition, leukocyte trafficking and leukocyte endothelium adhesion. The complex and prolonged immunomodulation induced by this drug contributes to its efficacy in solid organ transplantation, mainly by reducing the incidence of acute graft rejection. PMID:26722653

  4. Characterization of a cashew allergen, 11S globulin (Ana o 2), conformational epitope.

    Science.gov (United States)

    Robotham, Jason M; Xia, Lixin; Willison, LeAnna N; Teuber, Suzanne S; Sathe, Shridhar K; Roux, Kenneth H

    2010-05-01

    Both linear and conformational epitopes likely contribute to the allergenicity of tree nut allergens, yet, due largely to technical issues, few conformational epitopes have been characterized. Using the well studied recombinant cashew allergen, Ana o 2, an 11S globulin or legumin, we identified a murine monoclonal antibody which recognizes a conformational epitope and competes with patient IgE Ana o 2-reactive antibodies. This epitope is expressed on the large subunit of Ana o 2, but only when associated with an 11S globulin small subunit. Both Ana o 2 and the homologous soybean Gly m 6 small subunits can foster epitope expression, even when the natural N-terminal to C-terminal subunit order is reversed in chimeric molecules. The epitope, which is also expressed on native Ana o 2, is readily susceptible to destruction by physical and chemical denaturants. PMID:20362336

  5. Soy 11S Globulin Acid Subunits as the Novel Food Polymer Carrier

    Directory of Open Access Journals (Sweden)

    Lei Chen

    2015-01-01

    Full Text Available Aflatoxins were conjugated with soy 11S globulins acid subunits and the hapten-specific monoclonal antibodies (McAbs cross-reactive with four major aflatoxins were achieved using indirect competitive ELISA screening procedure. The two antibodies (clones 1B2 and 2D3 had similar reaction efficiency with aflatoxins B1, B2, and G1 but showed a weak cross-reaction to G2. The clone 4C5 exhibited the highest sensitivity for all four aflatoxins. The concentrations of aflatoxins B1, B2, G1, and G2 at 50% inhibition for 4C5 were 1.1, 1.2, 2.1, and 17.6 pg mL−1. The results indicated that soy 11S globulin acid subunits were suitable novel carriers for aflatoxin antigen in immunization experiments and clone 4C5 could be used for simultaneous analysis of total aflatoxins.

  6. Human sex hormone–binding globulin variants associated with hyperandrogenism and ovarian dysfunction

    OpenAIRE

    Hogeveen, Kevin N.; Cousin, Patrice; Pugeat, Michel; Dewailly, Didier; Soudan, Benoît; Hammond, Geoffrey L.

    2002-01-01

    The access of testosterone and estradiol to target tissues is regulated by sex hormone–binding globulin (SHBG) in human blood. Serum SHBG levels are low in patients with hyperandrogenism, especially in association with polycystic ovarian syndrome (PCOS) and in individuals at risk for diabetes and heart disease. Here, we identify SHBG coding region variations from a compound heterozygous patient who presented with severe hyperandrogenism during pregnancy. Serum SHBG levels in this patient meas...

  7. Rapid irreversible encephalopathy associated with anti-D immune globulin treatment for idiopathic thrombocytopenic purpura.

    Science.gov (United States)

    Christopher, Kenneth; Horkan, Clare; Barb, Ilie T; Arbelaez, Christian; Hodgdon, Travis A; Yodice, Paul C

    2004-11-01

    Intravenous Rho (D) immune globulin (IV RhIG, WinRho SDF) has been shown to be a safe treatment for idiopathic thrombocytopenic purpura (ITP). Common side effects of IV RhIG include mild hemolysis, febrile reaction, and headache. Significant hemolysis with renal impairment following IV RhIG has been reported. We report a case of irreversible encephalopathy 48 hr following an infusion of IV RhIG for treatment of ITP. PMID:15495245

  8. Relationship between Post-kidney Transplantation Antithymocyte Globulin Therapy and Wound Healing Complications

    OpenAIRE

    2012-01-01

    Background: Wound healing disorders are probably the most common post-transplantation surgical complications. It is thought that wound healing disturbance occurs due to antiproliferative effects of immunosuppressive drugs. On the other hand, success of transplantation is dependent on immunosuppressive therapies. Antihuman thymocyte globulin (ATG) has been widely used as induction therapy but the impact of this treatment on wound healing is not fully understood. Objective: To investigate wound...

  9. Genetic variants of sex hormone-binding globulin and their biological consequences

    OpenAIRE

    2009-01-01

    Abstract Several hormonal and metabolic factors have been found to influence the production of sex hormone-binding globulin (SHBG). In addition, twin studies have suggested that genetic factors may also contribute to variation in SHBG levels. Given the clinical significance of SHBG in regulating bioavailable sex steroid hormones, a number of studies examined the potential association between polymorphisms of SHBG gene and serum SHBG levels as well as their possible contribution in ...

  10. Identification of apolipoprotein A-I in the alpha-globulin fraction of avian plasma

    OpenAIRE

    Bed'hom, Bertrand; Guillot, Alain; Levrier, Julie; Chaste-Duvernoy, Daniel; Bomsel-Dementoy, Marie-Claude; Saint Jalme, Michel

    2009-01-01

    Background: Plasma protein electrophoresis is frequently used in birds as a tool for the diagnosis and monitoring of disease. Identification of proteins in individual peaks can help improve our understanding of changes in protein concentration in physiologic and pathologic conditions.[br/] Objective: The aim of this study was to verify the presence and identity the protein(s) in the prominent α-globulin peak of orange-winged parrots (Amazona amazonica), black kites (Milvus migrans), and rock ...

  11. Self-Assembly of Rice Bran Globulin Fibrils in Electrostatic Screening: Nanostructure and Gels

    OpenAIRE

    Lihua Huang; Yehui Zhang; Haibin Li

    2014-01-01

    The effects of various ionic strengths and protein concentrations on the fibrils structure and gel properties of rice bran globulin (RBG) at pH 2.0 were investigated using atomic force microscopy (AFM), rheometer, and scanning electron microscope (SEM). AFM images showed the morphology of assembling RBG fibrils from strand beads to becoming branch clustered, when electrostatic repulsive forces attenuated gradually with increasing ionic strength. NaCl seems to accelerate the kinetics of fibril...

  12. Immune reconstitution after haematopoietic transplantation with two different doses of pre-graft antithymocyte globulin.

    Science.gov (United States)

    Duval, M; Pédron, B; Rohrlich, P; Legrand, F; Faye, A; Lescoeur, B; Bensaid, P; Larchee, R; Sterkers, G; Vilmer, E

    2002-10-01

    Antithymocyte globulin is widely used before haematopoietic transplantation with HLA-matched unrelated donors or mismatched relatives to prevent rejection and graft-versus-host disease (GVHD). However, optimal dosage is still under debate. Thirty-one consecutive children, mainly with haematological malignancies, were transplanted in a single institution with such donors, selected by HLA-A -B compatibility by serology and DRB1* by DNA typing. Antithymocyte globulin (Thymoglobuline; Sangstat) was infused at days -3, -2, -1. Total dosage varied: 16 patients received a median of 7.5 mg/kg (2.5 to 10.5: low-dose group), and 15 a median of 15.5 mg/kg (14.4 to 19.4: high-dose group). Post-transplant GVHD prophylaxis consisted of cyclosporine, short-course methotrexate and steroids. CD3(+), CD4(+) and CD19(+) cell reconstitution was slower in the high-dose group. Median time to reach 100 CD4(+) cells was 8 months vs 4 months (P = 0.03). Median time to normal CD19(+) cells was 16 months vs 8 months (P = 0.01). CD16(+)CD56(+) and CD8(+) cell reconstitution was similar. Nine patients in the high-dose group and two in the low-dose group experienced life-threatening opportunistic infections (P = 0.009). Although obtained from a limited number of patients, our data suggest that a higher pre-graft dose of antithymocyte globulin may negatively influence immune reconstitution.

  13. A Novel Anti-Human Syndecan-1(CD138) Monoclonal Antibody 4B3: Characterization and Application

    Institute of Scientific and Technical Information of China (English)

    Wanping Sun; Fengming Wang; Fang Xie; Guoqing Wang; Jin Sun; Gehua Yu; Yuhua Qiu; Xueguang Zhang

    2007-01-01

    Syndecan-1 (CD138), a member of integral membrane heparin sulfate proteoglycans, is an essential matrix receptor for maintaining the normal morphological phenotypes. In this study, we generated a specific mouse anti-human syndecan-1 monoclonal antibody (mAb) 4B3 and identified it by competition assay with the available syndecan-1 mAb (BB4). Stained by 4B3, the expression of syndecan-1 was detected on tumor cell lines, such as 8226,U266, XG-1, XG-2, Daudi and Jurkat. The expression was also found on neuron stem cells. It was established that 4B3 mAb could inhibit XG-1 and XG-2 proliferation. The data not only determined that 4B3 mAb was a functional anti-human syndecan-1 mAb, but also indicated that syndecan-1 might be a valuable surface antigen and play an important role in regulation of tumor pathology and differentiation of neural stem cells. This novel antibody 4B3 may be value of study of tumor proliferation/survival mechanism and contributes to diagnosis and treatment of diverse diseases.

  14. Protein chemical characterization of Gc globulin (vitamin D-binding protein) isoforms; Gc-1f, Gc-1s and Gc-2

    DEFF Research Database (Denmark)

    Christiansen, Maja; Jørgensen, Charlotte S; Laursen, Inga;

    2007-01-01

    -survival of patients with fulminant hepatic failure and trauma. Here, we characterize the dominant isoforms of plasma-derived Gc globulin from Cohn fraction IV paste with respect to amino acid sequence and posttranslational modifications. Gc globulin was purified in large scale and the isoforms separated by ion......Gc globulin, also called vitamin D-binding protein, is a plasma protein involved in the extracellular actin-scavenger system, vitamin D transport and possibly also other biological activities. Low levels of Gc globulin have been found to correlate with multiple organ failure and non...

  15. Anti-Human Endoglin (hCD105 Immunotoxin—Containing Recombinant Single Chain Ribosome-Inactivating Protein Musarmin 1

    Directory of Open Access Journals (Sweden)

    Begoña Barriuso

    2016-06-01

    Full Text Available Endoglin (CD105 is an accessory component of the TGF-β receptor complex, which is expressed in a number of tissues and over-expressed in the endothelial cells of tumor neovasculature. Targeting endoglin with immunotoxins containing type 2 ribosome-inactivating proteins has proved an effective tool to reduce blood supply to B16 mice tumor xenografts. We prepared anti-endoglin immunotoxin (IT—containing recombinant musarmin 1 (single chain ribosome-inactivating proteins linked to the mouse anti-human CD105 44G4 mouse monoclonal antibody via N-succinimidyl 3-(2-pyridyldithio propionate (SPDP. The immunotoxin specifically killed L929 fibroblast mouse cells transfected with the short form of human endoglin with IC50 values in the range of 5 × 10−10 to 10−9 M.

  16. WinRho: Rh immune globulin prepared by ion exchange for intravenous use.

    OpenAIRE

    Bowman, J. M.; Friesen, A D; Pollock, J. M.; Taylor, W E

    1980-01-01

    An Rh immune globulin [Rh IgG] for intravenous use, WinRho, has been prepared by the Winnipeg Rh Institute by a modification of the ion-exchange column method of Hoppe and colleagues. When administered to Rh-negative male and nonpregnant female volunteers WinRho was found to be nonpyrogenic, nontoxic, safe and protective against Rh alloimmunization. In a clinical trial with 240 microgram given at about 28 weeks' gestation and 120 microgram given after delivery to Rh-negative women at risk of ...

  17. Rapid encephalopathy associated with anti-D immune globulin treatment for idiopathic thrombocytopenic purpura.

    Science.gov (United States)

    Golla, Sunitha; Horkan, Clare; Dogaru, Grigore; Teske, Thomas E; Christopher, Kenneth

    2008-01-01

    Rho (D) immune globulin intravenous (IV RhIG, WinRho SDF) has been shown to be a safe treatment for idiopathic thrombocytopenic purpura. Common side effects of IV RhIG include mild hemolysis, febrile reaction and headache. Significant hemolysis with renal impairment is infrequently noted. A single case of irreversible encephalopathy following IV RhIG has been reported. We report a second case of encephalopathy following an infusion of IV RhIG for treatment of idiopathic thrombocytopenic purpura. PMID:18957844

  18. Proposal of abolition of the skin sensitivity test before equine rabies immune globulin application

    OpenAIRE

    Cupo, Palmira; AZEVEDO-MARQUES Marisa M. de; SARTI Willy; HERING Sylvia Evelyn

    2001-01-01

    An epizootic outbreak of rabies occurred in 1995 in Ribeirão Preto, SP, with 58 cases of animal rabies (54 dogs, 3 cats and 1 bat) confirmed by the Pasteur Institute of São Paulo, and one human death. The need to provide care to a large number of people for the application of equine rabies immune globulin (ERIG) prevented the execution of the skin sensitivity test (SST) and often also the execution of desensitization, procedures routinely used up to that time at the Emergency Unit of the Univ...

  19. 11S Storage globulin from pumpkin seeds: regularities of proteolysis by papain.

    Science.gov (United States)

    Rudakova, A S; Rudakov, S V; Kakhovskaya, I A; Shutov, A D

    2014-08-01

    Limited proteolysis of the α- and β-chains and deep cleavage of the αβ-subunits by the cooperative (one-by-one) mechanism was observed in the course of papain hydrolysis of cucurbitin, an 11S storage globulin from seeds of the pumpkin Cucurbita maxima. An independent analysis of the kinetics of the limited and cooperative proteolyses revealed that the reaction occurs in two successive steps. In the first step, limited proteolysis consisting of detachments of short terminal peptides from the α- and β-chains was observed. The cooperative proteolysis, which occurs as a pseudo-first order reaction, started at the second step. Therefore, the limited proteolysis at the first step plays a regulatory role, impacting the rate of deep degradation of cucurbitin molecules by the cooperative mechanism. Structural alterations of cucurbitin induced by limited proteolysis are suggested to generate its susceptibility to cooperative proteolysis. These alterations are tentatively discussed on the basis of the tertiary structure of the cucurbitin subunit pdb|2EVX in comparison with previously obtained data on features of degradation of soybean 11S globulin hydrolyzed by papain. PMID:25365492

  20. EVALUATION OF IL-6 PRODUCTION BY HUMAN BLOOD CELLS INCUBATED WITH METAL COMPLEXES OF Γ-GLOBULIN

    OpenAIRE

    S. B. Cheknev; I. E. Efremova; A. S. Mezdrokhina; A. A. Babajanz

    2014-01-01

    Abstract. This study has shown that that a common cytokine pool induced in cultured human peripheral blood cells (PBC) supplied by either γ-globulin fraction proteins, copper or zinc cations, or appropriate metal complexes, contains detectable amounts of IL-6 (0.39+0.14 to 2.04+0.16 ng/ml). γ-globulin complexes with zinc or copper ions are able to induce production of IL-6 in amounts differing from those induced by control proteins, or copper and zinc ions used alone. IL-6 production by PBC i...

  1. Trends in anti-D immune globulin for childhood immune thrombocytopenia: Usage, response rates, and adverse effects

    OpenAIRE

    Long, Michelle; Kalish, Leslie A.; Neufeld, Ellis J.; Grace, Rachael F.

    2011-01-01

    In 2010, the Food and Drug Administration (FDA) added a black box warning to anti-D immune globulin (Rho(D) immune globulin, anti-D) for immune thrombocytopenia (ITP) to warn of the complications related to severe hemolysis. The objective of this retrospective medical record review was to examine recent trends in anti-D use to treat ITP and rates of adverse events in a single large pediatric hematology program. Over a 7-year period, 176 (35%) of 502 ITP patients at our center received anti-D....

  2. Preparation and functional studies of hydroxyethyl chitosan nanoparticles loaded with anti-human death receptor 5 single-chain antibody

    Directory of Open Access Journals (Sweden)

    Yang J

    2014-05-01

    Full Text Available Jingjing Yang,1,3,* Xiaoping Huang,1,3,* Fanghong Luo,1 Xiaofeng Cheng,3 Lianna Cheng,3 Bin Liu,4 Lihong Chen,2 Ruyi Hu,1,3 Chunyan Shi,1,3 Guohong Zhuang,1,3 Ping Yin2 1Anti-Cancer Research Center, Medical College, Xiamen University, Fujian, People's Republic of China, 2The Department of Pathology, Zhongshan Hospital, Xiamen University, Xiamen, People's Republic of China, 3Organ transplantation institution, Xiamen University, Xiamen, People's Republic of China, 4Jilin Vocational College of Industry and Technology, Jilin, People's Republic of China  *These authors contributed equally to this work Objective: To prepare hydroxyethyl chitosan nanoparticles loaded with anti-human death receptor 5 single-chain antibody, and study their characteristics, functions, and mechanisms of action. Materials and methods: The anti-human death receptor 5 single-chain antibody was constructed and expressed. Protein-loaded hydroxyethyl chitosan nanoparticles were prepared, and their size, morphology, particle-size distribution and surface zeta potential were measured by scanning electron microscopy and laser particle-size analysis. Mouse H22 hepatocellular carcinoma cells were cultured, and growth inhibition was examined using the CellTiter-Blue cell-viability assay. Flow cytometry and Hoechst 33342 were employed to measure cell apoptosis. Kunming mice with H22 tumor models were treated with protein-loaded hydroxyethyl chitosan nanoparticles, and their body weight and tumor size were measured, while hematoxylin and eosin staining was used to detect antitumor effects in vivo and side effects from tumors. Results: The protein-loaded hydroxyethyl chitosan nanoparticles had good stability; the zeta potential was -24.2±0.205, and the dispersion index was 0.203. The inhibition of the protein-loaded hydroxyethyl chitosan nanoparticles on H22 growth was both time- and dose-dependent. Increased expressions of active caspase 8, active caspase 3, and BAX were detected

  3. Guidelines on the use of intravenous immune globulin for hematologic conditions.

    Science.gov (United States)

    Anderson, David; Ali, Kaiser; Blanchette, Victor; Brouwers, Melissa; Couban, Stephen; Radmoor, Paula; Huebsch, Lothar; Hume, Heather; McLeod, Anne; Meyer, Ralph; Moltzan, Catherine; Nahirniak, Susan; Nantel, Stephen; Pineo, Graham; Rock, Gail

    2007-04-01

    Canada's per capita use of intravenous immune globulin (IVIG) grew by approximately 115% between 1998 and 2006, making Canada one of the world's highest per capita users of IVIG. It is believed that most of this growth is attributable to off-label usage. To help ensure IVIG use is in keeping with an evidence-based approach to the practice of medicine, the National Advisory Committee on Blood and Blood Products of Canada (NAC) and Canadian Blood Services convened a panel of national experts to develop an evidence-based practice guideline on the use of IVIG for hematologic conditions. The mandate of the expert panel was to review evidence regarding use of IVIG for 18 hematologic conditions and formulate recommendations on IVIG use for each. A panel of 13 clinical experts and 1 expert in practice guideline development met to review the evidence and reach consensus on the recommendations for the use of IVIG. The primary sources used by the panel were 3 recent evidence-based reviews. Recommendations were based on interpretation of the available evidence and where evidence was lacking, consensus of expert clinical opinion. A draft of the practice guideline was circulated to hematologists in Canada for feedback. The results of this process were reviewed by the expert panel, and modifications to the draft guideline were made where appropriate. This practice guideline will provide the NAC with a basis for making recommendations to provincial and territorial health ministries regarding IVIG use management. Specific recommendations for routine use of IVIG were made for 7 conditions including acquired red cell aplasia; acquired hypogammaglobulinemia (secondary to malignancy); fetal-neonatal alloimmune thrombocytopenia; hemolytic disease of the newborn; HIV-associated thrombocytopenia; idiopathic thrombocytopenic purpura; and posttransfusion purpura. Intravenous immune globulin was not recommended for use, except under certain life-threatening circumstances, for 8 conditions

  4. Konsentrasi Protein Total, Albumin, dan Globulin Anak Kambing Peranakan Etawah Setelah Pemberian Berbagai Sediaan Kolostrum* (TOTAL PROTEIN, ALBUMIN, AND GLOBULIN CONCENTRATIONS ON ETTAWAH CROSSBREED NEONATES FOLLOWING THE ADMINISTRATION OF VARIOUS FORM O

    Directory of Open Access Journals (Sweden)

    Anita Esfandiari

    2014-10-01

    Full Text Available This experiment was conducted to study the profile of total protein, albumin, and globulin concentrationson Ettawah crossbreed neonates after consuming various colostrums. Twenty four healthy neonatal kidswere used in this study. The neonates were divided into four groups. Each group received fresh maternal(goat colostrum, frozen-thawed bovine colostrum, bovine spray dried colostrum, and bovine powdercommercial colostrum, respectively. Colostrums were given at 10% of body weight directly after birth andfollowed by the same amount every 12 hours, for three days. The blood was taken from jugular vein at 0, 12,24, 48, 72, and 168 hours after birth to determine total protein, albumin, and globulin concentrations.Results of this study indicated that the serum total protein and globulin concentration increased andreached the peak at 24 hours after birth. Compared to the concentration at birth, the increase of totalprotein concentration were 62.77%, 59.26%, 48.05%, and 66.67% in fresh maternal (goat, frozen-thawedbovine, bovine spray dried, and commercial bovine colostrum, respectively. Serum globulin concentrationincreased 4.9, 4.4, 4.8, and 14.6 times in fresh matermnal goat, frozen-thawed bovine, spray dried, andcommercial bovine colostrums respectively, compared to the concentration at birth. In conclusion, theconsumption of various colostrums i.e. fresh maternal goat colostrums, bovine colostrums (frozen-thawed,spray dried and commercial colostrums would increase the concentration of blood total protein and globulin,which both reached the highest concentration at 24 h after birth.

  5. Self-Assembly of Nano Hydroxyapatite or Aragonite Induced by Molecular Recognition to Soy Globulin 7S or 11S.

    Science.gov (United States)

    Liu, Dagang; Tian, Huafeng; Kumar, Rakesh; Zhang, Lina

    2009-09-01

    Molecular self-assembly is emerging as a viable 'bottom-up' approach to build stable organic/inorganic nanometer-scale blocks. Herein, under the conditions of appropriate pH and ionic strength, soy globulin 7S or 11S were coprecipitated with hydroxyapatite (HAp) or aragonite (Arag), respectively, to fabricate two organic/inorganic hybrids: 7S/HAp and 11S/Arag. Results from high-resolution transmission electron microscopy show that the hybrids exhibit a nanosized core-shell structure with globulin monomer 7S or 11S as core and HAp or Arag as shells. 7S/HAp and 11S/Arag present a disk and hexagon shape, respectively. After calcinations, monodispersed HAp without support from globulins existed as nanospheres. It was revealed that the globulin as host induces the self-assembly and growth layer by layer of HAp or Arag nanocrystals. The factors of molecular recognition and surface potential definitely affected the size and shape of the hierarchical blocks. This work provided a novel pathway to controllably synthesize a wide variety of precise plant protein/biomineral hybrid biomaterials.

  6. Effect of gonadotropins and alpha 2u-globulin on testicular steroidogenesis and spermatogenesis in melatonin-treated rats

    Directory of Open Access Journals (Sweden)

    R Biswas

    2012-09-01

    Full Text Available Administration of melatonin (400?g/100g bd.wt. for 14 days caused a fall in weights of the testes and accessory sex organs and testicular 17?-hydroxysteroid dehydrogenase (17?-HSD but rise in 3?-hydroxysteroid dehydrogenase (3?-HSD activity, decreased spermatogenesis, serum level of gonadotropins, testosterone and alpha 2u-globulin, The animals treated with melatonin when received gonadotropins or alpha 2u-globulin for the last seven days reversed the weight of testis and accessory sex organs, 3?-HSD, 17?-HSD activities, serum level of gonadotropins, testosterone and alpha 2u-globulin when compared with melatonin-treated rats. It is concluded that alpha 2u-globulin prevents testicular degeneration in melatonin-treated rats by stimulating the synthesis of gonadotropins. Journal of College of Medical Sciences-Nepal,2012,Vol-8,No-1, 7-12 DOI: http://dx.doi.org/10.3126/jcmsn.v8i1.6819

  7. Detection of opsonic antibodies against Enterococcus faecalis cell wall carbohydrates in immune globulin preparations.

    Science.gov (United States)

    Hufnagel, M; Sixel, K; Hammer, F; Kropec, A; Sava, I G; Theilacker, C; Berner, R; Huebner, J

    2014-08-01

    Three different commercially available polyvalent immune globulins (IG) were investigated for the existence of antibodies against cell wall carbohydrates of four different E. faecalis serotypes (using a cell wall carbohydrate-enzyme-linked immunosorbent assay), and whether these antibodies mediated opsonic killing (using an opsonic-killing assay). All three IG preparations contained antibodies against all four serotypes (CPS-A to CPS-D). However, only one of the three IG preparations showed opsonic killing against all four serotypes. Average killing was higher against serotypes A and B (72 and 79 %, respectively) than against serotypes C and D (30 and 37 %, respectively). Such IG preparations could play a role as an adjuvant therapeutic option in life-threatening infections with E. faecalis, particularly when resistant strains are involved.

  8. Case reports: treatment of nevirapine-associated dress syndrome with intravenous immune globulin (IVIG).

    Science.gov (United States)

    Fields, Katherine S; Petersen, Marta J; Chiao, Elizabeth; Tristani-Firouzi, Payam

    2005-01-01

    Drug rash with eosinophilia and systemic symptoms (DRESS) syndrome is an adverse drug reaction most commonly associated with aromatic antiepileptic agents. It is characterized by the triad of skin eruption, fever, and systemic involvement, with the latter usually manifesting as hepatitis and lymphadenopathy. Mortality is primarily due to hepatic failure and can be as high as 10%. Formerly referred to by names such as Dilantin hypersensitivity syndrome and anticonvulsant hypersensitivity syndrome, DRESS syndrome is a more precise term since this reaction pattern can be seen with other agents. DRESS syndrome has also been reported in association with sulfonamides, allopurinol, terbinafine, minocycline, azathioprine, and dapsone as well as with several antiretroviral agents such as abacavir and nevirapine. We describe a patient with HIV who developed nevirapine hypersensitivity syndrome who was successfully treated with intravenous immune globulin (IVIG). PMID:16004028

  9. Antithymocyte globulin-induced acute respiratory distress syndrome after renal transplantation: a case report

    Institute of Scientific and Technical Information of China (English)

    TU Guo-wei; JU Min-jie; XU Ming; RONG Rui-ming; ZHU Tong-yu; LUO Zhe

    2012-01-01

    Antithymocyte globulin (ATG) has long been used for immune-induction and anti-rejection treatments for solid organ transplantations.To date,few cases of ATG-induced acute respiratory distress syndrome (ARDS) have been published.Here,we present a case of ARDS caused by a single low-dose of ATG in a renal transplant recipient and the subsequent treatments administered.Although the patient suffered from ARDS and delayed graft function,he was successfully treated.We emphasize that the presence of such complications should be considered when unexplained respiratory distress occurs.Early use of corticosteroids,adjustment of immunosuppressive regimens,and conservative fluid management,as well as empiric antimicrobial therapies,may be effective strategies for the treatment of ARDS caused by ATG.

  10. Fasting induces the generation of serum thyronine-binding globulin in Zucker rats

    International Nuclear Information System (INIS)

    Five-month-old lean and obese Zucker rats were fasted for up to 7 days (lean rats) or 28 days (obese rats), and serum total and free T4 and T3 concentrations, percent free T4 and T3 by equilibrium dialysis, and the binding of [125I] T4 to serum proteins by gel electrophoresis were measured. In the lean rats, a 4- or 7-day fast resulted in significant decreases in serum total and free T4 and T3 concentrations. There was a decrease in the percent free T3 after 7 days of starvation. In contrast, a 4- or 7-day fast did not alter any of these variables in the obese rats. However, after 14 or more days of starvation, serum total T4 and T3 concentrations increased, and the percent free T4 and T3 decreased, resulting in no change in the serum free T4 or T3 concentrations in the obese rats. The percent of [125I]T4 bound to serum thyronine-binding globulin increased and the percent bound to thyronine-binding prealbumin decreased with the duration of the fast in both the lean and obese rats. The increase in serum thyronine-binding globulin binding of T4 can explain the increase in serum total T4 and T3 concentrations, the decrease in percent free T4 and T3, and the normal free hormone concentration in the long term fasted obese rats. The findings in the lean rats appear to be due to a combination of the known central hypothyroidism that occurs during 4-7 days of fasting and the fasting-induced changes in T4 binding in serum. Changes in T4 and T3 binding in serum during fasting in the rat must be considered when the effects of fasting on serum concentrations of the thyroid hormones, thyroid hormone kinetics, and the peripheral action of the thyroid hormones are evaluated

  11. Detection of α2u-globulin in rat pup preputial gland by Maldi-tof mass spectrometry

    Directory of Open Access Journals (Sweden)

    Ponnirul PONMANICKAM,Gnanasekaran JEBAMERCY, Govindaraju ARCHUNAN, Soundrapandian KANNAN

    2009-08-01

    Full Text Available The α2u-globulin, a soluble protein identified in the urine and preputial gland of adult male rat is reported to be pheromone carrier. The pup preputial gland plays a significant role in chemical communication for mother-young interaction; however, the presence of a pheromone-carrying protein in the pup preputial gland has not been confirmed. Therefore, the present study was carried out to identify the α2u-globulin in the pup preputial gland by Matrix Assisted laser Desorption Ionization Time of Flight mass spectrometry (MALDI-TOF. The preputial glands of prepubertal rats were subjected to one-dimensional SDS-PAGE. In-gel trypsin digestion of a 18 kDa band was carried out and analyzed by MALDI-TOF. The results of a MASCOT search showed the presence of α2u-globulin in the18 kDa band. In contrast to the report of the synthesis of this protein only in adult rats, the identification of this protein in pup preputial gland is significant. The results suggest that synthesis of α2u-globulin in the rat preputial gland starts in the prepubertal stage itself. In prepubertal rats, the preputial gland is a source of pheromone for performing anogenital licking behaviour by the mother rat. Since α2u-globulin belongs to the lipocalin (ligand carrier family, it might carry the volatile for processing pheromonal communication in mother-pup bonding in rat [Current Zoology 55(4: 296–300, 2009].

  12. Biophysical and immunological studies on bovine immune globulins with evidence for selective transport within the mammary gland from maternal plasma to colostrum

    Science.gov (United States)

    Pierce, A. E.; Feinstein, A.

    1965-01-01

    Three immune globulins in maternal serum and colostrum and newly born calf serum, have been characterized and compared. An examination was made to determine first, which of the maternal serum immune globulins accumulate in the circulation of the calf and secondly, the selectivity of the mammary gland for these proteins compared with the intestinal mucosa of the newly born calf. By difference in their electrophoretic mobilities three antigenically related immune globulins were isolated from bovine serum. The immune lactoglobulins in bovine colostrum were qualitatively similar to those in serum. However, marked differences were observed between the relative concentrations in serum and colostrum of the three immune globulins. An electrophoretically fast immune globulin (C1), present in colostrum at high concentration, was shown to be antigenically similar to an immune globulin (S1) present in the maternal serum at low concentration. These findings indicate that the mammary gland showed a highly selective preference for, and hence ability to concentrate in, colostrum, the electrophoretically fastest serum immune globulin. The slowest serum immune globulin and the component with intermediate electrophoretic mobility (S3 and S2 respectively) were both present at high concentration in bovine maternal serum, but were transmitted at different rates into the colostrum, so that the slowest serum immune globulin (S3) was present in the colostrum as a comparatively minor component (C3). In contrast to the mammary gland, the intestine of the newly born calf (permeable to undegraded protein during the first 24 hours of life) showed no selectivity. Immune globulins showing the three electrophoretic mobilities were absorbed equally readily. Thus, while the bovine mammary gland showed a highly selective preference for certain electrophoretically different serum proteins, no comparable selectivity was shown by the intestinal mucosa of the newly born calf. The results emphasize the

  13. Cetuximab in combination with anti-human IgG antibodies efficiently down-regulates the EGF receptor by macropinocytosis

    Energy Technology Data Exchange (ETDEWEB)

    Berger, Christian [Department of Pathology, Oslo University Hospital, Rikshospitalet, Post box 4950 Nydalen, 0424 Oslo (Norway); Madshus, Inger Helene [Institute of Pathology, University of Oslo, Rikshospitalet, 0027 Oslo (Norway); Department of Pathology, Oslo University Hospital, Rikshospitalet, Post box 4950 Nydalen, 0424 Oslo (Norway); Stang, Espen, E-mail: espsta@rr-research.no [Department of Pathology, Oslo University Hospital, Rikshospitalet, Post box 4950 Nydalen, 0424 Oslo (Norway)

    2012-12-10

    The monoclonal antibody C225 (Cetuximab) blocks binding of ligand to the epidermal growth factor receptor (EGFR). In addition, it is known that incubation with C225 induces endocytosis of the EGFR. This endocytosis has previously been shown to be increased when C225 is combined with an additional monoclonal anti-EGFR antibody. However, the effects of antibody combinations on EGFR activation, endocytosis, trafficking and degradation have been unclear. By binding a secondary antibody to the C225-EGFR complex, we here demonstrate that a combination of antibodies can efficiently internalize and degrade the EGFR. Although the combination of antibodies activated the EGFR kinase and induced ubiquitination of the EGFR, the kinase activity was not required for internalization of the EGFR. In contrast to EGF-induced EGFR down-regulation, the antibody combination efficiently degraded the EGFR without initiating downstream proliferative signaling. The antibody-induced internalization of EGFR was found not to depend on clathrin and/or dynamin, but depended on actin polymerization, suggesting induction of macropinocytosis. Macropinocytosis may cause internalization of large membrane areas, and this could explain the highly efficient internalization of the EGFR induced by combination of antibodies. -- Highlight: Black-Right-Pointing-Pointer Cetuximab induced endocytosis of EGFR increases upon combination with anti-human IgG. Black-Right-Pointing-Pointer Antibody combination causes internalization of EGFR by macropinocytosis. Black-Right-Pointing-Pointer Antibody-induced internalization of EGFR is independent of EGFR kinase activity. Black-Right-Pointing-Pointer Antibody combination may have a zipper effect and cross-link EGFRs on neighboring cells.

  14. Cetuximab in combination with anti-human IgG antibodies efficiently down-regulates the EGF receptor by macropinocytosis

    International Nuclear Information System (INIS)

    The monoclonal antibody C225 (Cetuximab) blocks binding of ligand to the epidermal growth factor receptor (EGFR). In addition, it is known that incubation with C225 induces endocytosis of the EGFR. This endocytosis has previously been shown to be increased when C225 is combined with an additional monoclonal anti-EGFR antibody. However, the effects of antibody combinations on EGFR activation, endocytosis, trafficking and degradation have been unclear. By binding a secondary antibody to the C225-EGFR complex, we here demonstrate that a combination of antibodies can efficiently internalize and degrade the EGFR. Although the combination of antibodies activated the EGFR kinase and induced ubiquitination of the EGFR, the kinase activity was not required for internalization of the EGFR. In contrast to EGF-induced EGFR down-regulation, the antibody combination efficiently degraded the EGFR without initiating downstream proliferative signaling. The antibody-induced internalization of EGFR was found not to depend on clathrin and/or dynamin, but depended on actin polymerization, suggesting induction of macropinocytosis. Macropinocytosis may cause internalization of large membrane areas, and this could explain the highly efficient internalization of the EGFR induced by combination of antibodies. -- Highlight: ► Cetuximab induced endocytosis of EGFR increases upon combination with anti-human IgG. ► Antibody combination causes internalization of EGFR by macropinocytosis. ► Antibody-induced internalization of EGFR is independent of EGFR kinase activity. ► Antibody combination may have a zipper effect and cross-link EGFRs on neighboring cells.

  15. Monoclonal anti-human factor VII antibodies. Detection in plasma of a second protein antigenically and genetically related to factor VII.

    OpenAIRE

    Broze, G J; S. Hickman; Miletich, J P

    1985-01-01

    Several murine monoclonal anti-human Factor VII antibodies were produced using hybridoma technology. Two noncompetitive monoclonal antibodies were used to examine by Western blotting the Factor VII cross-reactive material (CRM) in normal human plasma and three commercially available congenitally Factor VII-deficient plasmas, and to construct a facile "sandwich" immunoassay for plasma Factor VII. A second, previously undescribed, form of Factor VII CRM was detected in human plasma, which on We...

  16. Biocompatibility of Solid-Dosage Forms of Anti-Human Immunodeficiency Virus Type 1 Microbicides with the Human Cervicovaginal Mucosa Modeled Ex Vivo▿

    OpenAIRE

    Trifonova, Radiana T.; Pasicznyk, Jenna-Malia; Fichorova, Raina N.

    2006-01-01

    Topical anti-human immunodeficiency virus (HIV) microbicides are being sought to reduce the spread of HIV type 1 (HIV-1) during sexual intercourse. The success of this strategy depends upon the selection of formulations compatible with the natural vaginal mucosal barrier. This study applied ex vivo-modeled human cervicovaginal epithelium to evaluate experimental solid-dosage forms of the anti-HIV-1 microbicide cellulose acetate 1,2-benzenedicarboxylate (CAP) and over-the-counter (OTC) vaginal...

  17. Proposal of abolition of the skin sensitivity test before equine rabies immune globulin application

    Directory of Open Access Journals (Sweden)

    CUPO Palmira

    2001-01-01

    Full Text Available An epizootic outbreak of rabies occurred in 1995 in Ribeirão Preto, SP, with 58 cases of animal rabies (54 dogs, 3 cats and 1 bat confirmed by the Pasteur Institute of São Paulo, and one human death. The need to provide care to a large number of people for the application of equine rabies immune globulin (ERIG prevented the execution of the skin sensitivity test (SST and often also the execution of desensitization, procedures routinely used up to that time at the Emergency Unit of the University Hospital of the Faculty of Medicine of Ribeirão Preto, University of São Paulo (EU-UHFMRP-USP, a reference hospital for the application of heterologous sera. In view of our positive experience of several years with the abolition of SST and of the use of premedication before the application of antivenom sera, we used a similar schedule for ERIG application. Of the 1489 victims of animal bites, 1054 (71% received ERIG; no patient was submitted to SST and all received intravenously anti-histamines (anti-H1 + anti-H2 and corticosteroids before the procedure. The patients were kept under observation for 60 to 180 minutes and no adverse reaction was observed. On the basis of these results, since December 1995 ERIG application has been decentralized in Ribeirão Preto and has become the responsibility of the Emergency Unit of the University Hospital and the Central Basic Health Unit, where the same routine is used. Since then, 4216 patients have received ERIG (1818 at the Basic Health Unit and 2398 at the EU-UHFMRP, with no problems. The ideal would be the routine use of human rabies immune globulin (HRIG in public health programs, but this is problematic, because of their high cost. However, while this does not occur, the use of SST is no longer justified at the time of application of ERIG, in view of the clinical evidence of low predictive value and low sensitivity of SST involving the application of heterologous sera. It is very important to point out

  18. Thyroid hormones and thyroxine-binding globulin in relation to liver function and serum testosterone in men with alcoholic cirrhosis

    DEFF Research Database (Denmark)

    Becker, U; Gluud, C; Bennett, Patrick

    1988-01-01

    In 73 euthyroid male patients with histologically verified alcoholic cirrhosis, thyroid hormones, thyroxine-binding globulin (TBG) and testosterone concentrations (total, non-protein- and non-SHBG-bound) were studied in relation to each other and to the degree of liver dysfunction. Serum concentr......In 73 euthyroid male patients with histologically verified alcoholic cirrhosis, thyroid hormones, thyroxine-binding globulin (TBG) and testosterone concentrations (total, non-protein- and non-SHBG-bound) were studied in relation to each other and to the degree of liver dysfunction. Serum...... correlated significantly (Kendall Tau-beta = -0.33, p = 0.001) with total serum testosterone concentrations, while there was a negative correlation (Kendall Tau-beta = -0.20, p = 0.025) between testosterone and TSH values. No correlation was found between testosterone concentrations and serum levels of TBG...

  19. Update on the use of intravenous immune globulin in the treatment of patients with inflammatory muscle disease.

    Science.gov (United States)

    Dalakas, M C

    1995-11-01

    The inflammatory myopathies consist of three distinct groups: dermatomyositis, polymyositis, and inclusion body myositis. Dermatomyositis is distinguished by its characteristic rash, while polymyositis is a diagnosis of exclusion. Inclusion body myositis is characterized by early involvement of distal muscle groups and the quadriceps. Definitive diagnosis is made by muscle biopsy, which demonstrates histological features characteristic for each disorder. Immune mechanisms play a role in the pathogenesis of the inflammatory myopathies. A complement-mediated microangiopathy is seen in dermatomyositis, while there is evidence for a T cell-mediated process in polymyositis and inclusion body myositis. Treatment with prednisone is helpful to a majority of patients for a period of time. Immunosuppressive drugs have met with limited success. We describe a group of patients with dermatomyositis, resistant to available therapies, whose muscle strength, skin changes, and muscle biopsies improved significantly during treatment with intravenous immune globulin. The treatment of polymyositis and inclusion body myositis with intravenous immune globulin is currently under study.

  20. Effect of Anthrax Immune Globulin on Response to BioThrax (Anthrax Vaccine Adsorbed) in New Zealand White Rabbits

    OpenAIRE

    Malkevich, Nina V.; Basu, Subhendu; Rudge, Thomas L.; Clement, Kristin H.; Chakrabarti, Ajoy C.; Aimes, Ronald T.; Nabors, Gary S.; Skiadopoulos, Mario H.; Ionin, Boris

    2013-01-01

    Development of anthrax countermeasures that may be used concomitantly in a postexposure setting requires an understanding of the interaction between these products. Anthrax immune globulin intravenous (AIGIV) is a candidate immunotherapeutic that contains neutralizing antibodies against protective antigen (PA), a component of anthrax toxins. We evaluated the interaction between AIGIV and BioThrax (anthrax vaccine adsorbed) in rabbits. While pharmacokinetics of AIGIV were not altered by vaccin...

  1. Retrospective diagnosis of Q fever in a country abattoir by the use of specific IgM globulin estimations

    Energy Technology Data Exchange (ETDEWEB)

    Murphy, A.M.; Hunt, J.G.

    1981-10-03

    Twenty-two cases of pyrexial illness which occurred amongst workers in a country abattoir were investigated retrospectively for Q fever, brucellosis, and leptospirosis. In 18, the illness was shown to be Q fever. No diagnoses were established for the other four. The demonstration of circulating Q-fever-specific IgM globulin was instrumental in establishing the diagnosis in many of the cases.

  2. Oestradiol and sex hormone-binding globulin in premenopausal and post-menopausal meat-eaters, vegetarians and vegans

    OpenAIRE

    Thomas, H V; Davey, G. K.; Key, T.J.

    1999-01-01

    Endogenous oestradiol is strongly associated with breast cancer risk but its determinants are poorly understood. To test the hypothesis that vegetarians have lower plasma oestradiol and higher sex hormone-binding globulin (SHBG) than meat-eaters we assayed samples from 640 premenopausal women (153 meat-eaters, 382 vegetarians, 105 vegans) and 457 post-menopausal women (223 meat-eaters, 196 vegetarians, 38 vegans). Vegetarians and vegans had lower mean body mass indices (BMI) and lower plasma ...

  3. Effect of weight reduction on insulin sensitivity, sex hormone-binding globulin, sex hormones and gonadotrophins in obese children

    DEFF Research Database (Denmark)

    Birkebaek, N H; Lange, Aksel; Holland-Fischer, P;

    2010-01-01

    Obesity in men is associated with reduced insulin sensitivity and hypoandrogenism, while obesity in women is associated with reduced insulin sensitivity and hyperandrogenism. In children, the effect of obesity and weight reduction on the hypothalamo-pituitary-gonadal axis is rarely investigated. ....... The aim of the present study was to investigate the effect of weight reduction in obese Caucasian children on insulin sensitivity, sex hormone-binding globulin (SHBG), DHEAS and the hypothalamo-pituitary-gonadal axis....

  4. The effect of anti-human plasminogen monoclonal antibodies on Glu-plasminogen activation by plasminogen activators

    Directory of Open Access Journals (Sweden)

    M. Akrami

    2006-07-01

    Full Text Available Background: Human plasminogen is a plasma glycoprotein synthesized mainly in the liver. Conversion of plasminogen to plasmin by plasminogen activators is a key event in the fibrinolytic system. In this study, we investigated the effects of two anti-human plasminogen monoclonal antibodies, A1D12 and MC2B8 on Glu-plasminogen activation in presence of u-PA, t-PA and streptokinase. Methods: Producing of Hybridoma antibodies was performed by fusion of spleen cells from BALB/C mice immunized with Glu-plasminogen and NS1 myeloma cells. Antibody binding to Human Glu-plasminogen was assessed using an ELISA assay. Activation of plasminogen was determined by measuring plasmin generation using the chromogenic substrate S-2251 and the effect of monoclonal antibodies, A1D12 and MC2B8 on plasminogen activation in solution was then evaluated. Initial rates and kinetic parameters of plasminogen activation in the presence of monoclonal antibodies were calculated. The effect of the monoclonal antibody MC2B8 on the rate of plasmin hydrolysis was measured. The effect of F(ab'2 fragment of A1D12 on u-PA catalyzed-plasminogen activation also compared with the effect of the whole antibody in this reaction. Results: ELISA assay showed that the antibodies reacted well with antigens. A1D12 increased the maximum velocity (Vmax of plasminogen activation by each of the three plasminogen activators and MC2B8 decreased it. In all activation reactions, the KM value of plasminogen activation did not significantly change in the presence of antibody A1D12 whereas antibody MC2B8 increased the KM value of plasminogen activation by u-PA, fibrin monomer dependent t-PA and streptokinase. Monoclonal antibody MC2B8 had no significant effect on plasmin hydrolysis rate of synthetic substrate S-2251. Activation rate of plasminogen by u-PA in the lower concentration of F (ab2 fragment of A1D12 was identical to activation in the presence of the whole antibody. Conclusion: The binding of

  5. Yeast-recombinant hepatitis B vaccine: efficacy with hepatitis B immune globulin in prevention of perinatal hepatitis B virus transmission

    International Nuclear Information System (INIS)

    A yeast-recombinant hepatitis B vaccine was licensed recently by the Food and Drug administration and is now available. To assess the efficacy of the yeast-recombinant vaccine, the authors administered the vaccine in combination with hepatitis B immune globulin to high-risk newborns. If infants whose mothers were positive for both hepatitis B surface antigen and the e antigen receive no immunoprophylaxis, 70% to 90% become infected with the virus, and almost all become chronic carriers. Among infants in this study who received hepatitis B immune globulin at birth and three 5-+g doses of yeast-recombinant hepatitis B vaccine, only 4.8% became chronic carriers, a better than 90% level of protection and a rate that is comparable with that seen with immune globulin and plasma-derived hepatitis B vaccine. Hepatitis surface antigen and antibodies were detected by radioimmunoassay. These data suggest that, in this high-risk setting, the yeast-recombinant vaccine is as effective as the plasma-derived vaccine in preventing hepatitis B virus infection and the chronic carrier state

  6. A gene encoding a vicilin-like protein is specifically expressed in fern spores. Evolutionary pathway of seed storage globulins.

    Science.gov (United States)

    Shutov, A D; Braun, H; Chesnokov, Y V; Bäumlein, H

    1998-02-15

    The isolation and characterisation of a cDNA coding for a vicilin-like protein of the fern Matteuccia struthiopteris is described. The corresponding gene is specifically expressed during late stages of spore development. Extensive sequence comparisons suggest that the fern protein can be considered as a molecular missing link between single-domain germin/spherulin-like proteins and two-domain seed storage globulins of gymnosperms and angiosperms. Further, evidence is provided for the existence of a superfamily of structurally related, functionally different proteins which includes storage globulins of the vicilin and legumin families, a membrane-associated sucrose-binding protein of soybean, a Forssman antigen-binding lectin of velvet bean, the precursor of the vacuolar membrane bound proteins MP27/MP32 of pumpkin, the embryogenesis-specific protein Gea8 of carrot, the fern-spore-specific protein described here as well as the functionally diverse family of germins/germin-like proteins and the spherulins of myxomycetes. We propose that seed storage globulins of spermatophytes evolved from desiccation-related single-domain proteins of prokaryotes via a duplicated two-domain ancestor that is best represented by the extant fern spore-specific vicilin-like protein.

  7. Effects of storage time on total protein and globulin concentrations in bovine fresh frozen plasma obtained for transfusion.

    Science.gov (United States)

    Proverbio, D; Spada, E; Baggiani, L; Bagnagatti De Giorgi, G; Roggero, N; Belloli, A; Pravettoni, D; Perego, R

    2015-01-01

    To evaluate the effects of storage conditions on total protein (TP) and globulin fractions in fresh frozen bovine plasma units prepared and stored for transfusion, TP and globulin fractions were evaluated in fresh plasma and at 1 month and 6 and 12 months after blood collection in plasma stored at -20°C. Significant differences in concentrations were found in the median concentration of total protein (P=0.0336), between 0 months and 1 month (P=0.0108), 0 and 6 months (P=0.0023), and 0 and 12 months (P=0.0027), in mean concentration (g/dL) of albumin (P=0.0394), between 0 months and 1 month (P=0.0131), 0 and 6 months (P=0.0035), and 0 and 12 months (P=0.0038), and beta-2 fraction (P=0.0401), between 0 and 6 months (P=0.0401) and 0 and 12 months (P=0.0230). This study suggests that total gamma globulin concentration in bovine frozen plasma is stable for 12 months at -20°C. Total protein, ALB, and beta-2 fraction have significantly different concentrations (g/dL) when compared to prestorage. This study has shown IgG protein fraction stability in bovine fresh frozen plasma collected for transfusion; therefore, bovine fresh frozen plasma seems to be suitable for the treatment of hypogammaglobulinemia (failure of passive transfer) in calves when stored for 12 months at -20°C.

  8. Circulating immune complexes, complement activation kinetics and serum sickness following treatment with heterologous anti-snake venom globulin.

    Science.gov (United States)

    Nielsen, H; Sørensen, H; Faber, V; Svehag, S E

    1978-01-01

    Consecutive serum and plasma samples, from a patient receiving 100 ml polyvalent horse anti-venom globulin after a rattlesnake bite, were analysed for circulating immune complexes (IC) and activation of complement factors. IC were determined by two independent methods, a complement consumption assay and a Clq-binding assay. Rapidly rising levels of complement-fixing circulating IC were detected as early as 4--5 days after the serum treatment and distinct IC-activity was recorded in both assays on day 8 when clinical symptoms of serum sickness were observed. The IC remained in circulation for at least 5 weeks. Signs of intravascular C-activation in the form of low C3, C4 and C5 values was noted on day 1 after treatment. Factor B was demonstrable 3--4 days after the snake bite and this factor and C3c attained a peak around day 8, just before maximal suppression of native C3 and C4. 14 days after the globulin treatment C3c and B were declining rapidly while C3 and C4 approached normal values first 36 days after treatment. An increase in heterophilic antibodies to sheep erythrocytes was observed after treatment with anti-venom globulin. PMID:635471

  9. Female hyperandrogenemia and normal serum levels of testosterone and sex hormone binding globulin

    Directory of Open Access Journals (Sweden)

    Karina Danilowicz

    2014-10-01

    Full Text Available It is well known that the reference values usually employed for endocrine biochemical measurements are those suggested by the suppliers of commercial kits despite their advice that each laboratory should set its own reference values. Our objectives were to (i determine reference ranges for serum testosterone (T and sex hormone binding globulin (SHBG appropriate to our laboratory and population, and (ii to analyze their influence on evaluating hyperandrogenemia. SHBG and T were measured, and free and bioavailable testosterone calculated, in (a 30 selected non-hyperandrogenic women, (b 87 non-selected healthy female blood donors, (c 53 women with hyperandrogenism, and (d 38 women with hyperandrogenic disorders but without biochemical hyperandrogenemia according to normal ranges suggested by the kit manufacturer. Mean serum SHBG concentrations were significantly different among all four groups. SHBG levels were significantly higher in selected normal women (group a. Using our results for this selected control group as new reference values, 12 out of 38 (31.6% women with hyperandrogenic disorders without apparent hyperandrogenemia (group d were recategorized as hyperandrogenemic. Similarly, 4 out of 63 (6.4% non-selected, normal weight, women (group b, were recategorized as hyperandrogenic. Therefore, the diagnosis of hyperandrogenemia would improve accuracy by using customized reference SHBG values instead of those suggested by the suppliers.

  10. Corticosteroid-Binding Globulin: A Review of Basic and Clinical Advances.

    Science.gov (United States)

    Meyer, E J; Nenke, M A; Rankin, W; Lewis, J G; Torpy, D J

    2016-06-01

    Corticosteroid-binding globulin (CBG, transcortin) is the primary cortisol binding protein. It is a non-inhibitory serine protease inhibitor, capable of conformational change from a high cortisol-binding affinity form to a low affinity form upon cleavage of its reactive centre loop by various proteases, such as neutrophil elastase. The burgeoning inflammatory role of CBG applies to acute, severe inflammation where depletion is associated with mortality, and to chronic inflammation where defects in cortisol delivery may perpetuate inflammation. Naturally occurring human mutations influence a wide range of CBG properties and point toward a role in hitherto unexplained chronic musculoskeletal pain and fatigue disorders as well as potentially affecting fertility outcomes including offspring gender. In vitro and knock-out animal models of CBG propose a role for CBG in cortisol transport to the brain, providing a foundation for understanding the human observations in those with CBG mutations and sex differences in stress-related mood and behaviour. Finally, CBG measurement has a practical role in the estimation of free cortisol, useful in clinical circumstances where CBG levels or cortisol binding affinity is reduced. Taken together, novel data suggest a role for cortisol in targeted cortisol delivery, with implications in acute and chronic inflammation, as well as roles in metabolism and neurocognitive function, implying that CBG is a multifaceted component in the mechanisms of hypothalamic-pituitary-adrenal axis related homeostasis. PMID:27214312

  11. A corm-specific gene encodes tarin, a major globulin of taro (Colocasia esculenta L. Schott).

    Science.gov (United States)

    Bezerra, I C; Castro, L A; Neshich, G; de Almeida, E R; de Sá, M F; Mello, L V; Monte-Neshich, D C

    1995-04-01

    A gene encoding a globulin from a major taro (Colocasia esculenta L. Schott) corm protein family, tarin (G1, ca. 28 kDa) was isolated from a lambda Charon 35 library, using a cDNA derived from a highly abundant corm-specific mRNA, as probe. The gene, named tar1, and the corresponding cDNA were characterized and compared. No introns were found. The major transcription start site was determined by primer extension analysis. The gene has an open reading frame (ORF) of 765 bp, and the deduced amino acid sequence indicated a precursor polypeptide of 255 residues that is post-translationally processed into two subunits of about 12.5 kDa each. The deduced protein is 45% homologous to curculin, a sweet-tasting protein found in the fruit pulp of Curculigo latifolia and 40% homologous to a mannose-binding lectin from Galanthus nivalis. Significant similarity was also found at the nucleic acid sequence level with genes encoding lectins from plant species of the Amaryllidaceae and Lilliaceae families.

  12. Clinical benefits and immunopathological correlates of intravenous immune globulin in the treatment of inflammatory myopathies.

    Science.gov (United States)

    Dalakas, M C

    1996-05-01

    High-dose intravenous immune globulin (IVIG) is emerging as a promising therapy for patients with inflammatory myopathies who have become unresponsive to, or cannot tolerate, conventional therapies. In a double-blind, placebo-controlled study, using objective criteria for improvement, IVIG demonstrated moderate to dramatic improvement in 75% of the patients with dermatomyositis. Preliminary results from a controlled study in inclusion-body myositis show that IVIG may also exert a mild benefit, but only in a small number of patients and in certain muscle groups. In some patients with polymyositis, IVIG is reported to be of benefit but controlled studies have not yet been completed. Immunocytochemical, immunological and in vitro studies on the patients' repeated muscle biopsies and follow-up sera showed that IVIG exerts its action in inflammatory myopathies by: (i) inhibiting myotoxic cytokines, such as TNF-alpha and IL-1; (ii) blockade of Fc receptors on endomysial macrophages interfering with Fc receptor-mediated phagocytosis; and (iii) inhibiting the uptake of C3 and intercepting the formation and deposition of membranolytic attack complex on the endomysial capillaries.

  13. Localization of sex hormone binding globulin in the rat vomeronasal organ.

    Science.gov (United States)

    Ploß, V M; Gebhart, V M; Gisder, D; Dölz, W; Jirikowski, G F

    2014-11-01

    Volatile and non-volatile derivates of gonadal steroids are known to act as pheromones in many mammalian species. Pheromones have multiple effects on the brain via the olfactory system. Their primary port of entry seems to be the vomeronasal organ (VNO) but the underlying cellular and molecular mechanisms are unclear so far. Recently we localized sex hormone binding globulin (SHBG) in both the main and the accessory olfactory system of rat with immunocytochemistry and RT-PCR. The accessory olfactory system consisting of VNO and accessory olfactory bulb showed high expression of SHBG. In the present paper we studied SHBG expression in the VNO in greater detail. In semithin sections we found SHBG immunostaining in the perinuclear cytoplasm of some of the sensory neurons, in sensory cilia and in their axons. A portion of the basal cells and some of the goblet cells in the non-sensory epithelium showed intense SHBG staining. SHBG was abundant in exocrine cells of the vomeronasal glands, perhaps compartimentalized in secretory vesicles. In situ hybridization revealed specific signals in sensory and non-sensory cells of the VNO. Our findings indicate that SHBG expressed in the VNO may be liberated into nasal secretions to bind aerosolic steroids. SHBG in sensory cells may be involved in signaling actions of pheromones. PMID:25154024

  14. Relation of cigarette smoking in males of different ages to sex hormone binding globulin and testosterone

    International Nuclear Information System (INIS)

    The relationship of cigarette smoking, age, total testosterone free testosterone and sex hormone binding globulin (SHBG) were examined by solid phase radioimmunoassay in 90 randomly chosen healthy males of different ages. The serum levels of these hormones were investigated for smokers compared with non-smokers, of the same ages in 3 groups (adolescent males, middle aged males, and old aged males). Results indicated that cigarette smokers showed increased serum levels of testosterone (60.0% higher, P> 0.05), free testosterone (51.0 higher, P > 0.005) in young adolescent males group, testosterone (27.8% higher, P > 0.001), free testosterone (21.3% higher, P > 0.001) in middle aged males group, and testosterone (21.0% higher, P > 0.001), free testosterone (16.8% higher, P > 0.4) in old ages males group. SHBG was calculated as a mean of free and total testosterone in each group. smokers showed higher mean values of SHBG than non-smokers. Age was positively associated with serum SHBG, it was found that SHBG increased by 17.2% from the youngest (> 18 years) to the oldest age (> 65 years)

  15. Self-Assembly of Rice Bran Globulin Fibrils in Electrostatic Screening: Nanostructure and Gels

    Directory of Open Access Journals (Sweden)

    Lihua Huang

    2014-01-01

    Full Text Available The effects of various ionic strengths and protein concentrations on the fibrils structure and gel properties of rice bran globulin (RBG at pH 2.0 were investigated using atomic force microscopy (AFM, rheometer, and scanning electron microscope (SEM. AFM images showed the morphology of assembling RBG fibrils from strand beads to becoming branch clustered, when electrostatic repulsive forces attenuated gradually with increasing ionic strength. NaCl seems to accelerate the kinetics of fibrils formation, resulting in a significant increase in Th T fluorescence intensity. The increased ionic strengths promote particle size increasing and zeta potential decreasing synchronously. The percolation model G'~C-Cpn be used to calculate theoretical RBG gels concentration at various ionic strengths (0–500 mM, which decreased from 15.17 ± 0.63 to 2.26 ± 0.27 wt%. SEM images exhibited a granular mesh-like gel structure. A more homogenous structure occurred in low ionic strength. This study elucidates properties of RBG fibrils and gels as a bioactive material.

  16. Role of corticosteroid binding globulin in the fast actions of glucocorticoids on the brain.

    Science.gov (United States)

    Moisan, M P; Minni, A M; Dominguez, G; Helbling, J C; Foury, A; Henkous, N; Dorey, R; Béracochéa, D

    2014-03-01

    Corticosteroid binding globulin (CBG) is a glycoprotein synthesized in liver and secreted in the blood where it binds with a high affinity but low capacity glucocorticoid hormones, cortisol in humans and corticosterone in laboratory rodents. In mammals, 95% of circulating glucocorticoids are bound to either CBG (80%) or albumin (15%) and only the 5% free fraction is able to enter the brain. During stress, the concentration of glucocorticoids rises significantly and the free fraction increases even more because CBG becomes saturated. However, glucocorticoids unbound to CBG are cleared from the blood more quickly. Our studies on mice totally devoid of CBG (Cbg k.o.) showed that during stress these mutant mice display a lower rise of glucocorticoids than the wild-type controls associated with altered emotional reactivity. These data suggested that CBG played a role in the fast actions of glucocorticoids on behavior. Further analyses demonstrated that stress-induced memory retrieval impairment, an example of the fast action of glucocorticoids on the brain is abolished in the Cbg k.o. mice. This effect of stress on memory retrieval could be restored in the Cbg k.o. mice by infusing corticosterone directly in the hippocampus. The mechanisms explaining these effects involved an increased clearance but no difference in corticosterone production. Thus, CBG seems to have an important role in maintaining in blood a glucocorticoid pool that will be able to access the brain for the fast effects of glucocorticoids.

  17. In silico identification of anthropogenic chemicals as ligands of zebrafish sex hormone binding globulin

    International Nuclear Information System (INIS)

    Anthropogenic compounds with the capacity to interact with the steroid-binding site of sex hormone binding globulin (SHBG) pose health risks to humans and other vertebrates including fish. Building on studies of human SHBG, we have applied in silico drug discovery methods to identify potential binders for SHBG in zebrafish (Danio rerio) as a model aquatic organism. Computational methods, including; homology modeling, molecular dynamics simulations, virtual screening, and 3D QSAR analysis, successfully identified 6 non-steroidal substances from the ZINC chemical database that bind to zebrafish SHBG (zfSHBG) with low-micromolar to nanomolar affinities, as determined by a competitive ligand-binding assay. We also screened 80,000 commercial substances listed by the European Chemicals Bureau and Environment Canada, and 6 non-steroidal hits from this in silico screen were tested experimentally for zfSHBG binding. All 6 of these compounds displaced the [3H]5α-dihydrotestosterone used as labeled ligand in the zfSHBG screening assay when tested at a 33 μM concentration, and 3 of them (hexestrol, 4-tert-octylcatechol, and dihydrobenzo(a)pyren-7(8H)-one) bind to zfSHBG in the micromolar range. The study demonstrates the feasibility of large-scale in silico screening of anthropogenic compounds that may disrupt or highjack functionally important protein:ligand interactions. Such studies could increase the awareness of hazards posed by existing commercial chemicals at relatively low cost

  18. Testosterone is required for corticosteroid-binding globulin upregulation by morphine to be fully manifested.

    Science.gov (United States)

    Nock, B; Wich, M; Cicero, T J; O'Connor, L H

    2000-09-01

    We previously reported that morphine increases the concentration of corticosteroid-binding globulin (CBG) in blood of male, but not female, rats. This pronounced sexual dimorphism suggested that CBG upregulation by morphine might be androgen-dependent. In the current studies, we found that castration, whether performed just before or just after puberty or in adulthood, increased the concentration of CBG in adult male rats. Naltrexone did not prevent this increase and, therefore, it does not appear to be attributable to the release of endogenous opioids. Exposure to morphine for 1 week in adulthood increased ( approximately 100%) the concentration of CBG in intact, i.e., sham-castrated, males. The CBG levels of castrated rats treated with morphine did not differ from those of intact rats treated with morphine. However, because castration increased the concentration of CBG, the difference between the placebo and morphine groups decreased with time after castration. At 4 weeks after castration, the difference between the morphine and placebo groups (19%) was no longer statistically significant. Testosterone replacement prevented the rise in CBG levels following castration and maintained the magnitude of the difference between placebo and morphine-treated rats within the normal range. Thus, testosterone appears necessary for morphine effects on CBG to be fully manifested. PMID:11113500

  19. Globulin-platelet model predicts minimal fibrosis and cirrhosis in chronic hepatitis B virus infected patients

    Institute of Scientific and Technical Information of China (English)

    Xu-Dong Liu; Jian-Lin Wu; Jian Liang; Tao Zhang,; Qing-Shou Sheng

    2012-01-01

    AIM:To establish a simple model consisting of the routine laboratory variables to predict both minimal fibrosis and cirrhosis in chronic hepatitis B virus (HBV)-infected patients.METHODS:We retrospectively investigated 114 chronic HBV-infected patients who underwent liver biopsy in two different hospitals.Thirteen parameters were analyzed by step-wise regression analysis and correlation analysis.A new fibrosis index [globulin/platelet (GP) model] was developed,including globulin (GLOB) and platelet count (PLT).GP model =GLOB (g/mL) x 100/PLT (x 109/L).We evaluated the receiver operating characteristics analysis used to predict minimal fibrosis and compared six other available models.RESULTS:Thirteen clinical biochemical and hematological variables [sex,age,PLT,alanine aminotransferase,aspartate aminotransferase (AST),albumin,GLOB,total bilirubin (T.bil),direct bilirubin (D.bil),glutamyl transferase,alkaline phosphatase,HBV DNA and prothrombin time (PT)] were analyzed according to three stages of liver fibrosis (F0-F1,F2-F3 and F4).Bivariate Spearman's rank correlation analysis showed that six variables,including age,PLT,T.bil,D.bil,GLOB and PT,were correlated with the three fibrosis stages (FS).Correlation coefficients were 0.23,-0.412,0.208,0.220,0.314 and 0.212; and P value was 0.014,< 0.001,0.026,0.018,0.001 and 0.024,respectively.Univariate analysis revealed that only PLT and GLOB were significantly different in the three FS (PLT:F =11.772,P <0.001; GLOB:F =6.612,P =0.002).Step-wise multiple regression analysis showed that PLT and GLOB were also independently correlated with FS (R2 =0.237).By Spearman's rank correlation analysis,GP model was significantly correlated with the three FS (r =0.466,P < 0.001).The median values in F0-F1,F2-F3 and F4 were 1.461,1.720 and 2.634.Compared with the six available models (fibrosis index,AST-platelet ratio,FIB-4,fibrosis-cirrhosis index and age-AST model and age-PLT ratio),GP model showed a highest correlation

  20. Study on immobilizations of ovine anti-human IgG and MCAb against EHF on radiation-modified silicone films

    International Nuclear Information System (INIS)

    Films of silicone (silastic) were grafted by monomer acrylamide vis γ-radiation technology and then the ovine anti-human IgG, Epidemic hemorrhagic fever (EHF)-MCAb were immobilized on the silastic-AAM films with different grafting yields passthrough associate reactions. Measruements of relationships between grafting yields. Contents of immobilized antibodies and immunoactivities for immobilized silastic-AAM films were performed by using 125I method ELISA method was used to measure the immunoactivities for the immobilized monoantibody. The results showed that the antibodies used can be immobilized on radiation-grafted silicone films and this immobilization method has its potential significance in clinical practice

  1. What the world's religions teach, applied to vaccines and immune globulins.

    Science.gov (United States)

    Grabenstein, John D

    2013-04-12

    For millennia, humans have sought and found purpose, solace, values, understanding, and fellowship in religious practices. Buddhist nuns performed variolation against smallpox over 1000 years ago. Since Jenner developed vaccination against smallpox in 1796, some people have objected to and declined vaccination, citing various religious reasons. This paper reviews the scriptural, canonical basis for such interpretations, as well as passages that support immunization. Populous faith traditions are considered, including Hinduism, Buddhism, Jainism, Judaism, Christianity, and Islam. Subjects of concern such as blood components, pharmaceutical excipients of porcine or bovine origin, rubella strain RA 27/3, and cell-culture media with remote fetal origins are evaluated against the religious concerns identified. The review identified more than 60 reports or evaluations of vaccine-preventable infectious-disease outbreaks that occurred within religious communities or that spread from them to broader communities. In multiple cases, ostensibly religious reasons to decline immunization actually reflected concerns about vaccine safety or personal beliefs among a social network of people organized around a faith community, rather than theologically based objections per se. Themes favoring vaccine acceptance included transformation of vaccine excipients from their starting material, extensive dilution of components of concern, the medicinal purpose of immunization (in contrast to diet), and lack of alternatives. Other important features included imperatives to preserve health and duty to community (e.g., parent to child, among neighbors). Concern that 'the body is a temple not to be defiled' is contrasted with other teaching and quality-control requirements in manufacturing vaccines and immune globulins. Health professionals who counsel hesitant patients or parents can ask about the basis for concern and how the individual applies religious understanding to decision-making about

  2. Endocrine Disruption: Computational Perspectives on Human Sex Hormone-Binding Globulin and Phthalate Plasticizers.

    Directory of Open Access Journals (Sweden)

    Ishfaq A Sheikh

    Full Text Available Phthalates are a class of high volume production chemicals used as plasticizers for household and industrial use. Several members of this chemical family have endocrine disrupting activity. Owing to ubiquitous environmental distribution and exposure of human population at all stages of life, phthalate contamination is a continuous global public health problem. Clinical and experimental studies have indicated that several phthalates are associated with adverse effects on development and function of human and animal systems especially the reproductive system and exposures during pregnancy and early childhood are by far of utmost concern. Sex hormone-binding globulin (SHBG is a plasma carrier protein that binds androgens and estrogens and represents a potential target for phthalate endocrine disruptor function in the body. In the present study, the binding mechanism of the nine phthalates i.e. DMP, DBP, DIBP, BBP, DNHP, DEHP, DNOP, DINP, DIDP with human SHBG was delineated by molecular docking simulation. Docking complexes of the nine phthalates displayed interactions with 15-31 amino acid residues of SHBG and a commonality of 55-95% interacting residues between natural ligand of SHBG, dihydrotestosterone, and the nine phthalate compounds was observed. The binding affinity values were more negative for long chain phthalates DEHP, DNOP, DINP, and DIDP compared to short chain phthalates such as DMP and DBP. The Dock score and Glide score values were also higher for long chain phthalates compared to short chain phthalates. Hence, overlapping of interacting amino acid residues between phthalate compounds and natural ligand, dihydrotestosterone, suggested potential disrupting activity of phthalates in the endocrine homeostasis function of SHBG, with long chain phthalates expected to be more potent than the short chain phthalates.

  3. Endocrine Disruption: Computational Perspectives on Human Sex Hormone-Binding Globulin and Phthalate Plasticizers.

    Science.gov (United States)

    Sheikh, Ishfaq A; Turki, Rola F; Abuzenadah, Adel M; Damanhouri, Ghazi A; Beg, Mohd A

    2016-01-01

    Phthalates are a class of high volume production chemicals used as plasticizers for household and industrial use. Several members of this chemical family have endocrine disrupting activity. Owing to ubiquitous environmental distribution and exposure of human population at all stages of life, phthalate contamination is a continuous global public health problem. Clinical and experimental studies have indicated that several phthalates are associated with adverse effects on development and function of human and animal systems especially the reproductive system and exposures during pregnancy and early childhood are by far of utmost concern. Sex hormone-binding globulin (SHBG) is a plasma carrier protein that binds androgens and estrogens and represents a potential target for phthalate endocrine disruptor function in the body. In the present study, the binding mechanism of the nine phthalates i.e. DMP, DBP, DIBP, BBP, DNHP, DEHP, DNOP, DINP, DIDP with human SHBG was delineated by molecular docking simulation. Docking complexes of the nine phthalates displayed interactions with 15-31 amino acid residues of SHBG and a commonality of 55-95% interacting residues between natural ligand of SHBG, dihydrotestosterone, and the nine phthalate compounds was observed. The binding affinity values were more negative for long chain phthalates DEHP, DNOP, DINP, and DIDP compared to short chain phthalates such as DMP and DBP. The Dock score and Glide score values were also higher for long chain phthalates compared to short chain phthalates. Hence, overlapping of interacting amino acid residues between phthalate compounds and natural ligand, dihydrotestosterone, suggested potential disrupting activity of phthalates in the endocrine homeostasis function of SHBG, with long chain phthalates expected to be more potent than the short chain phthalates. PMID:26963243

  4. What the world's religions teach, applied to vaccines and immune globulins.

    Science.gov (United States)

    Grabenstein, John D

    2013-04-12

    For millennia, humans have sought and found purpose, solace, values, understanding, and fellowship in religious practices. Buddhist nuns performed variolation against smallpox over 1000 years ago. Since Jenner developed vaccination against smallpox in 1796, some people have objected to and declined vaccination, citing various religious reasons. This paper reviews the scriptural, canonical basis for such interpretations, as well as passages that support immunization. Populous faith traditions are considered, including Hinduism, Buddhism, Jainism, Judaism, Christianity, and Islam. Subjects of concern such as blood components, pharmaceutical excipients of porcine or bovine origin, rubella strain RA 27/3, and cell-culture media with remote fetal origins are evaluated against the religious concerns identified. The review identified more than 60 reports or evaluations of vaccine-preventable infectious-disease outbreaks that occurred within religious communities or that spread from them to broader communities. In multiple cases, ostensibly religious reasons to decline immunization actually reflected concerns about vaccine safety or personal beliefs among a social network of people organized around a faith community, rather than theologically based objections per se. Themes favoring vaccine acceptance included transformation of vaccine excipients from their starting material, extensive dilution of components of concern, the medicinal purpose of immunization (in contrast to diet), and lack of alternatives. Other important features included imperatives to preserve health and duty to community (e.g., parent to child, among neighbors). Concern that 'the body is a temple not to be defiled' is contrasted with other teaching and quality-control requirements in manufacturing vaccines and immune globulins. Health professionals who counsel hesitant patients or parents can ask about the basis for concern and how the individual applies religious understanding to decision-making about

  5. Anti-thymocyte globulin-induced hyperbilirubinemia in patients with myelofibrosis undergoing allogeneic hematopoietic cell transplantation.

    Science.gov (United States)

    Ecsedi, Matyas; Schmohl, Jörg; Zeiser, Robert; Drexler, Beatrice; Halter, Jörg; Medinger, Michael; Duyster, Justus; Kanz, Lothar; Passweg, Jakob; Finke, Jürgen; Bethge, Wolfgang; Lengerke, Claudia

    2016-10-01

    Allogeneic hematopoietic cell transplantation (allo-HCT) remains the only curative treatment option for myelofibrosis (MF) despite the emergence of novel targeted therapies. To reduce graft rejection and graft-versus-host disease (GvHD), current allo-HCT protocols often include in vivo T lymphocyte depletion using polyclonal anti-thymocyte globulin (ATG). Shortly after ATG administration, an immediate inflammatory response with fever, chills, and laboratory alterations such as cytopenias, elevation of serum C-reactive protein, bilirubin, and transaminases can develop. Here, we explore whether MF patients, who commonly exhibit extramedullary hematopoiesis in the liver, might be particularly susceptible to ATG-induced liver toxicity. To test this hypothesis, we analyzed 130 control and 94 MF patients from three transplant centers treated with or without ATG during the allo-HCT conditioning regimen. Indeed, hyperbilirubinemia was found in nearly every MF patient treated with ATG (MF-ATG 54/60 = 90 %) as compared to non-ATG treated MF (MF-noATG 15/34 = 44.1 %, p < 0.001) and respectively ATG-treated non-MF patients of the control group (control-ATG, 43/77 = 56 %, p < 0.001). In contrast, transaminases were only inconsistently elevated. Hyperbilirubinemia was in most cases self-limiting and not predictive of increased incidence of non-relapse mortality, hepatic sinusoidal obstruction syndrome (SOS) or liver GvHD. In sum, awareness of this stereotypic bilirubin elevation in MF patients treated with ATG provides a relatively benign explanation for hyperbilirubinemia occurring in these patients during the early transplant. However, attention to drug levels of biliary excreted drugs is warranted, since altered bile flow may influence their clearance and enhance toxicity (e.g., busulfan, antifungal agents). PMID:27480090

  6. WinRho: Rh immune globulin prepared by ion exchange for intravenous use.

    Science.gov (United States)

    Bowman, J M; Friesen, A D; Pollock, J M; Taylor, W E

    1980-12-01

    An Rh immune globulin [Rh IgG] for intravenous use, WinRho, has been prepared by the Winnipeg Rh Institute by a modification of the ion-exchange column method of Hoppe and colleagues. When administered to Rh-negative male and nonpregnant female volunteers WinRho was found to be nonpyrogenic, nontoxic, safe and protective against Rh alloimmunization. In a clinical trial with 240 microgram given at about 28 weeks' gestation and 120 microgram given after delivery to Rh-negative women at risk of Rh immunization WinRho was effective in preventing Rh immunization. Of the 870 women carrying Rh-positive fetuses who were treated with WinRho during pregnancy and were not tested several months after delivery 14 would have shown evidence of Rh immunization by the time of delivery if WinRho had been ineffective; none showed such evidence. Of the 1122 women carrying Rh-positive fetuses who were retested 4 to 6 months after delivery 83 would have shown evidence of Rh immunization at that time if WinRho had been ineffective; only 1 showed such evidence. The efficiency of yield of anti-D with the modified method of production, the fct that it can be given intravenously (a route that causes the patient less discomfort and immediately results in high anti-D levels) and the lower levels of contaminating IgA and IgM make WinRho the preparation of choice for preventing Rh immunization. PMID:6161687

  7. Intravenous immune globulin in hereditary inclusion body myopathy: a pilot study

    Directory of Open Access Journals (Sweden)

    Dorward Heidi

    2007-01-01

    Full Text Available Abstract Background Hereditary Inclusion Body Myopathy (HIBM is an autosomal recessive, adult onset, non-inflammatory neuromuscular disorder with no effective treatment. The causative gene, GNE, codes for UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase, which catalyzes the first two reactions in the synthesis of sialic acid. Reduced sialylation of muscle glycoproteins, such as α-dystroglycan and neural cell adhesion molecule (NCAM, has been reported in HIBM. Methods We treated 4 HIBM patients with intravenous immune globulin (IVIG, in order to provide sialic acid, because IgG contains 8 μmol of sialic acid/g. IVIG was infused as a loading dose of 1 g/kg on two consecutive days followed by 3 doses of 400 mg/kg at weekly intervals. Results For all four patients, mean quadriceps strength improved from 19.0 kg at baseline to 23.2 kg (+22% directly after IVIG loading to 25.6 kg (+35% at the end of the study. Mean shoulder strength improved from 4.1 kg at baseline to 5.9 kg (+44% directly after IVIG loading to 6.0 kg (+46% at the end of the study. The composite improvement for 8 other muscle groups was 5% after the initial loading and 19% by the end of the study. Esophageal motility and lingual strength improved in the patients with abnormal barium swallows. Objective measures of functional improvement gave variable results, but the patients experienced improvements in daily activities that they considered clinically significant. Immunohistochemical staining and immunoblotting of muscle biopsies for α-dystroglycan and NCAM did not provide consistent evidence for increased sialylation after IVIG treatment. Side effects were limited to transient headaches and vomiting. Conclusion The mild benefits in muscle strength experienced by HIBM patients after IVIG treatment may be related to the provision of sialic acid supplied by IVIG. Other sources of sialic acid are being explored as treatment options for HIBM.

  8. Lack of association between Rh status, Rh immune globulin in pregnancy and autism.

    Science.gov (United States)

    Miles, Judith H; Takahashi, T Nicole

    2007-07-01

    Though causes of autism are considered largely genetic, considerable concern remains that exposure to Rh immune globulin (RhIg), which until 2001 in the United States contained the preservative thimerosal, can cause autism. To determine whether mothers of children with autism are more likely to be Rh negative (Rh(-)) or to have received RhIg preserved with thimerosal, which is 49.6% ethyl mercury, we surveyed families of children with an autism spectrum disorder (ASD) ascertained through a University-based autism clinic considered free of ascertainment biases related to type of autism or severity. Between 2004 and 2006, 305 mothers of 321 children with an ASD agreed to participate in a telephone interview. Analysis of complete records including the blood group status and RhIg exposure of 214 families showed that Rh(-) status is no higher in mothers of children with autism than in the general population, exposure to antepartum RhIg, preserved with thimerosal is no higher for children with autism and pregnancies are no more likely to be Rh incompatible. This was also true for autism subgroups defined by behavioral phenotype, gender, IQ, regressive onset, head circumference, dysmorphology, birth status, essential, or complex phenotype. These findings support the consensus that exposure to ethylmercury in thimerosal is not the cause of the increased prevalence of autism. These data are important not only for parents in this country but also for the international health community where thimerosal continues to be used to preserve multi-dose vials which in turn makes vaccines affordable.

  9. Thyroxine binding to serum thyronine-binding globulin in thyroidectomized adult and normal neonatal rats

    International Nuclear Information System (INIS)

    The amount of tracer [125I]T4 bound to serum thyronine-binding globulin (TBG) was measured by polyacrylamide gel electrophoresis in adult thyroidectomized (TX) rats and normal 1-day to 4-week-old rat puts. Thyroidectomy was associated with the appearance of significant amounts of [125I]T4 binding to serum TBG in lean rats, but not in obese Zucker rats. Treatment of the TX rats in vivo with replacement doses of T4 prevented this increase in TBG binding, but enrichment of serum from TX rats with T4 did not. Significant amounts of tracer [125I]T4 binding to TBG was present in serum from 1- to 3-week-old normal rat pups, but not in 1-day- or 4-week-old pups. There were significantly higher levels of TBG binding of [125I]T4 in serum from 2-week-old rat pups raised in litters of 16 pups compared to those raised in litters of 4 pups. All manipulations that result in the appearance of TBG in rat serum also result in either weight loss or a slowing in the rate of growth, suggesting that the appearance of TBG in rat serum has a nutritional component. This possibility is further supported by the observations that increases in TBG binding of [125I]T4 are not found in obese Zucker rats fed a low protein-high carbohydrate diet for 14 days or fasted for 7 days, or after thyroidectomy, perhaps owing to the large stores of fuel in the obese rat

  10. Anti-thymocyte globulin induces neoangiogenesis and preserves cardiac function after experimental myocardial infarction.

    Directory of Open Access Journals (Sweden)

    Michael Lichtenauer

    Full Text Available RATIONALE: Acute myocardial infarction (AMI followed by ventricular remodeling is the major cause of congestive heart failure and death in western world countries. OBJECTIVE: Of relevance are reports showing that infusion of apoptotic leucocytes or anti-lymphocyte serum after AMI reduces myocardial necrosis and preserves cardiac function. In order to corroborate this therapeutic mechanism, the utilization of an immunosuppressive agent with a comparable mechanism, such as anti-thymocyte globulin (ATG was evaluated in this study. METHODS AND RESULTS: AMI was induced in rats by ligation of the left anterior descending artery. Initially after the onset of ischemia, rabbit ATG (10 mg/rat was injected intravenously. In vitro and in vivo experiments showed that ATG induced a pronounced release of pro-angiogenic and chemotactic factors. Moreover, paracrine factors released from ATG co-incubated cell cultures conferred a down-regulation of p53 in cardiac myocytes. Rats that were injected with ATG evidenced higher numbers of CD68+ macrophages in the ischemic myocardium. Animals injected with ATG evidenced less myocardial necrosis, showed a significant reduction of infarct dimension and an improvement of post-AMI remodeling after six weeks (infarct dimension 24.9% vs. 11.4%, p<0.01. Moreover, a higher vessel density in the peri-infarct region indicated a better collateralization in rats that were injected with ATG. CONCLUSIONS: These data indicate that ATG, a therapeutic agent successfully applied in clinical transplant immunology, triggered cardioprotective effects after AMI that salvaged ischemic myocardium by down-regulation of p53. This might have raised the resistance against apoptotic cell death during ischemia. The combination of these mechanisms seems to be causative for improved cardiac function and less ventricular remodeling after experimental AMI.

  11. Effects of corticosterone pellets on baseline and stress-induced corticosterone and corticosteroid-binding-globulin.

    Science.gov (United States)

    Müller, Claudia; Almasi, Bettina; Roulin, Alexandre; Breuner, Creagh W; Jenni-Eiermann, Susanne; Jenni, Lukas

    2009-01-01

    Exogenous administration of glucocorticoids is a widely used and efficient tool to investigate the effects of elevated concentrations of these hormones in field studies. Because the effects of corticosterone are dose and duration-dependent, the exact course of plasma corticosterone levels after exogenous administration needs to be known. We tested the performance of self-degradable corticosterone pellets (implanted under the skin) in elevating plasma corticosterone levels. We monitored baseline (sampled within 3min after capture) total corticosterone levels and investigated potential interactions with corticosteroid-binding-globulin (CBG) capacity and the endogenous corticosterone response to handling in Eurasian kestrel Falco tinnunculus and barn owl Tyto alba nestlings. Corticosterone pellets designed for a 7-day-release in rodents elevated circulating baseline total corticosterone during only 2-3 days compared to placebo-nestlings. Highest levels occurred 1-2days after implantation and levels decreased strongly thereafter. CBG capacity was also increased, resulting in a smaller, but still significant, increase in baseline free corticosterone levels. The release of endogenous corticosterone as a response to handling was strong in placebo-nestlings, but absent 2 and 8 days after corticosterone pellet implantation. This indicates a potential shut-down of the hypothalamo-pituitary-adrenal axis after the 2-3 days of elevated baseline corticosterone levels. 20 days after pellet implantation, the endogenous corticosterone response to handling of nestlings implanted with corticosterone pellets attained similar levels as in placebo-nestlings. Self-degradable pellets proved to be an efficient tool to artificially elevate circulating baseline corticosterone especially in field studies, requiring only one intervention. The resulting peak-like elevation of circulating corticosterone, the concomitant elevation of CBG capacity, and the absence of an endogenous corticosterone

  12. Gene amplification as a cause of inherited thyroxine-binding globulin excess in two Japanese families

    Energy Technology Data Exchange (ETDEWEB)

    Mori, Yuichi; Miura, Yoshitaka; Saito, Hidehiko [Toyota Memorial Hospital (Japan)] [and others

    1995-12-01

    T{sub 4}-binding globulin (TBG) is the major thyroid hormone transport protein in man. Inherited abnormalities in the level of serum TBG have been classified as partial deficiency, complete deficiency, and excess. Sequencing analysis of the TBG gene, located on Xq21-22, has uncovered the molecular defects causing partial and complete deficiency. However, the mechanism leading to inherited TBG excess remains unknown. In this study, two Japanese families, F-A and F-T, with inherited TBG excess were analyzed. Serum TBG levels in hemizygous males were 58 and 44 {mu}g/mL, 3- and 2-fold the normal value, respectively. The molecule had normal properties in terms of heat stability and isoelectric focussing pattern. The sequence of the coding region and the promoter activity of the TBG gene were also indistinguishable between hemizygotes and normal subjects. The gene dosage of TBG relative to that of {beta}-globin, which is located on chromosome 11, and Duchenne muscular dystropy, which is located on Xp, was evaluated by coamplification of these target genes using polymerase chain reaction and subsequent quantitation by HPLC. The TBG/{beta}-globin ratios of the affected male and female of F-A were 3.13 and 4.13 times, respectively, that in the normal males. The TBG/Duchenne muscular dystrophy ratios were 2.92 and 2.09 times the normal value, respectively. These results are compatible with three copies of TBG gene on the affected X-chromosome. Similarly, a 2-fold increase in gene dosage was demonstrated in the affected hemizygote of F-T. A 3-fold tandem amplification of the TBG gene was shown by in situ hybridization of prometaphase and interphase chromosomes from the affected male with a biotinylated genomic TBG probe, confirming the gene dosage results. Gene amplification of TBG is the cause of inherited TBG excess in these two families. 35 refs., 3 figs., 2 tabs.

  13. Low-dose rabbit anti-thymoglobin globulin versus basiliximab for induction therapy in kidney transplantation

    Directory of Open Access Journals (Sweden)

    Himanshu V Patel

    2014-01-01

    Full Text Available We conducted a single-center prospective double-arm open-labeled study on kidney transplant patients from 2010 to 2011 to evaluate the efficacy of induction therapy using low, single-dose rabbit-antithymocyte globulin (r-ATG, 1.5 mg/kg on Day 0 (n = 80, 60 males, mean age 35.9 years, versus basiliximab (Interleukin-2 blocker 20 mg on Days 0 and 4 (n = 20, 12 males, mean age 45.1 years on renal allograft function in terms of serum creatinine (SCr, rejec-tion and infection episodes and patient/graft survival and cost. Demographic and post-transplant follow-up including immunosuppression was similar in both groups. In the r-ATG group, donors were unrelated (spouse, n = 25, deceased (n = 31 and parents/siblings (others, with a mean HLA match of 1.58. Donors in the basiliximab group were living unrelated (spouse, n = 15 and deceased (n = 5, with a mean HLA match of 1.56. No patient/graft was lost in the r-ATG group over a mean of one year follow-up, and the mean SCr was 1.28 mg/dL with 7.5% acute rejection (AR episodes; infections were also not observed. In the basiliximab group, over the same period of follow-up, there was 95% death-censored graft survival, and the mean SCr was 1.23 mg/dL with 10% AR episodes. One patient died due to bacterial pneumonia and one succumbed to coronary artery disease; one graft was lost due to uncontrolled acute humoral and cellular rejection. The cost of r-ATG and basiliximab were $600 and $2500, respectively. We conclude that induction immunosuppressive therapy with a low-dose r-ATG may be a better option as compared with basiliximab in terms of graft function, survival and cost benefit in kidney transplant patients.

  14. Biodistribution and radioimmunoimage of iodine-125 labeled anti-human ADAM15 polyclonal antibody in nude mice bearing human gastric carcinoma xenografts

    International Nuclear Information System (INIS)

    Objective: To investigate the biodistribution of Iodine-125 labeled anti-human ADAM15 polyclonal antibody in nude mice bearing human gastric carcinoma xenografts. Methods: The anti-human ADAM15 polyclonal antibody was labeled with I-125 using Chloramine-T method. The labeling efficiency and radiochemical purity of 125I-anti-ADAM15 antibody were measured. The SPECT planar imaging of nude mice bearing gastric carcinoma xenografts were performed at 1, 4, 8, 24, and 48 h post-injection and the biodistribution of 125I-anti-ADAM15 antibody was measured at 48 h after injection. Results: The labeling efficiency of 125I-anti-ADAM15 antibody was (75.16±9.43)% and its radiochemical purity was (99.44±0.21)% . Tumors could be cleanly visualized in SPECT planar images, and the radioactivity ratio of tumor to non-tumor tissue was 3.84±0.43 at 48 h post-injection. Conclusion: 125I-anti-ADAM15 antibody can target the gastric carcinoma in vivo, and provide good radioimmunoimages. (authors)

  15. Pretargeting vs. direct targeting of human betalox5 islet cells subcutaneously implanted in mice using an anti-human islet cell antibody

    International Nuclear Information System (INIS)

    Introduction: We previously demonstrated MORF/cMORF pretargeting of human islets and betalox 5 cells (a human beta cell line) transplanted subcutaneously in mice with the anti-human islet antibody, HPi1. We now compare pretargeting with direct targeting in the beta cell transplant model to evaluate the degree to which target/non-target (T/NT) ratios may be improved by pretargeting. Methods: Specific binding of an anti-human islet antibody HPi1 to the beta cells transplanted subcutaneously in mice was examined against a negative control antibody. We then compared pretargeting by MORF-HPi1 plus 111In-labeled cMORF to direct targeting by 111In-labeled HPi1. Results: HPi1 binding to betalox5 human cells in the transplant was shown by immunofluorescence. Normal organ 111In backgrounds by pretargeting were always lower, although target accumulations were similar. More importantly, the transplant to pancreas and liver ratios was, respectively, 26 and 10 by pretargeting as compared to 9 and 0.6 by direct targeting. Conclusions: Pretargeting greatly improves the T/NT ratios, and based on the estimated endocrine to exocrine ratio within a pancreas, pretargeting may be approaching the sensitivity required for successful imaging of human islets within this organ.

  16. Development of a complete human anti-human transferrin receptor C antibody as a novel marker of oral dysplasia and oral cancer

    International Nuclear Information System (INIS)

    Oral squamous cell carcinoma (OSCC) is the sixth most common cancer worldwide. Up to 20% of oral dysplasia cases have been suggested to undergo malignant transformation to OSCC; however, there are no methods to predict OSCC development. In this study, to identify the genes associated with oral dysplasia progression, we performed genomic copy number analyses of genomic DNA samples isolated from primary oral dysplasia and OSCC via the microdissection method and found elevated expression of transferrin receptor C (TfR1/TFRC) with genomic amplification in oral dysplasia and OSCC. The expression rate of TFRC in OSCC was significantly higher than that in dysplasia, suggesting that OSCC disease progression might be related to TFRC expression. Additionally, we investigated the in vitro and in vivo impacts of a newly established anti-human TFRC monoclonal antibody, which was isolated from a human cDNA library using the phage-display method, on cell proliferation and survival. The anti-TFRC antibody blocked the interaction between transferrin and TFRC and consequently inhibited iron uptake, leading to the iron deprivation-mediated suppression of cell growth and induction of apoptosis. Moreover, we demonstrated that the anti-TFRC antibody efficiently inhibited tumor growth in a murine xenograft OSCC model. Therefore, we suggest our developed complete human anti-human TFRC antibody as a useful, novel treatment for oral dysplasia and OSCC

  17. Clinical outcomes after hepatitis C infection from contaminated anti-D immune globulin. Irish Hepatology Research Group.

    LENUS (Irish Health Repository)

    Kenny-Walsh, E

    2012-02-03

    BACKGROUND AND METHODS: In February 1994, batches of anti-D immune globulin used in Ireland during 1977 and 1978 to prevent Rh isoimmunization were found to be contaminated with hepatitis C virus (HCV) from a single infected donor. In March 1994, a national screening program was initiated for all women who had received anti-D immune globulin between 1970 and 1994. Of the 62,667 women who had been screened when this study began, 704 (1.1 percent) had evidence of past or current HCV infection, and 390 of those 704 (55 percent) had positive tests for serum HCV RNA on reverse-transcription-polymerase-chain-reaction analysis. All 390 were offered a referral for clinical assessment and therapy. We evaluated 376 of these 390 women (96 percent); the other 14 were not seen at one of the designated treatment centers. RESULTS: The mean (+\\/-SD) age of the 376 women was 45+\\/-6 years at the time of screening. They had been infected with hepatitis C for about 17 years. A total of 304 women (81 percent) reported symptoms, most commonly fatigue (248 [66 percent]). Serum alanine aminotransferase concentrations were slightly elevated (40 to 99 U per liter) in 176 of 371 women (47 percent), and the concentrations were 100 U per liter or higher in 31 (8 percent). Liver biopsies showed inflammation in 356 of 363 women (98 percent); in most cases the inflammation was slight (41 percent) or moderate (52 percent). Although the biopsy samples from 186 of the 363 women (51 percent) showed evidence of fibrosis, only 7 women (2 percent) had probable or definite cirrhosis. Two of the seven reported excessive alcohol consumption. CONCLUSIONS: Most of the women with HCV infection 17 years after receiving HCV-contaminated anti-D immune globulin had evidence of slight or moderate hepatic inflammation on liver biopsy, about half had fibrosis, and 2 percent had probable or definite cirrhosis.

  18. IgE-binding epitopic peptide mapping on a three-dimensional model built for the 13S globulin allergen of buckwheat (Fagopyrum esculentum).

    Science.gov (United States)

    Sordet, Camille; Culerrier, Raphaël; Granier, Claude; Didier, Alain; Rougé, Pierre

    2009-06-01

    The three-dimensional model built for the 13S globulin allergen of buckwheat (Fagopyrum esculentum) consists of three protomers exhibiting the cupin motif, arranged in a homotrimer around a three-fold symmetry axis. Using the SPOT technique, 11 continuous IgE-binding epitopic peptides were characterized on the molecular surface of the 13S globulin allergen of buckwheat. Except for one of them, they all correspond to well exposed regions containing electropositiveley and/or electronegatively charged residues, which cover up to 40% of the molecular surface of the allergen. Some of these epitopes come in close contact to probably create more extended discontinuous epitopes, especially those located on the edge of the 13S globulin homotrimer. Half of the identified epitope peptides remain unaltered in a core structure protected against hydrolysis by digestive proteases and are thus assumed to promote the allergenicity of the 13S globulin. In addition, a few of these epitopes coincide with sequential IgE-binding epitopes previously characterized in soybean 11S globulins, that could account for the IgE-binding cross-reactions observed between soybean and buckwheat in Western blot experiments.

  19. Molecular characterization of a genetic variant of the steroid hormone-binding globulin gene in heterozygous subjects

    Energy Technology Data Exchange (ETDEWEB)

    Hardy, D.O.; Catterall, J.F. [Population Council, New York, NY (United States); Carino, C. [Instituto National de la Nutricion, Mexico City, MX (United States)] [and others

    1995-04-01

    Steroid hormone-binding globulin in human serum displays different isoelectric focusing (IEF) patterns among individuals, suggesting genetic variation in the gene for this extracellular steroid carrier protein. Analysis of allele frequencies and family studies suggested the existence of two codominant alleles of the gene. Subsequent determination of the molecular basis of a variant of the gene was carried out using DNA from homozygous individuals from a single Belgian family. It was of interest to characterize other variant individuals to determine whether all variants identified by IEF phenotyping were caused by the same mutation or whether other mutations occurred in the gene in different populations. Previous studies identified Mexican subjects who were heterozygous for the variant IEF phenotype. Denaturing gradient gel electrophoresis was used to localize the mutation in these subjects and to purify the variant allele for DNA sequence analysis. The results show that the mutation in this population is identical to that identified in the Belgian family, and no other mutations were detected in the gene. These data represent the first analysis of steroid hormone-binding globulin gene variation in heterozygous subjects and further support the conclusion of biallelism of the gene worldwide. 11 refs., 2 figs., 1 tab.

  20. Research progress on globulins of edible legumes%食用豆类球蛋白研究进展

    Institute of Scientific and Technical Information of China (English)

    刘红; 康玉凡

    2013-01-01

    This article analyzed and compared the storage protein content and composition of soybean (Glycine max L.),mung bean(Vigna radiate L. Wilczek),pea(Pisum staivum)and faba bean (Vicia faba)respectively. A summary was made on the structures and properties of the main globulin components:11S,7S and 8S. Extraction methods,functional properties and research trends of globulin were also discussed in this article to provide a theoretical and practical reference on the further study of legume proteins.%  该文比较分析大豆、绿豆、豌豆、蚕豆等蛋白含量及组成,就豆类蛋白主要成分―11S球蛋白、7S球蛋白和8S球蛋白亚基结构及性质,球蛋白提取方法及功能特性进行概述,并展望其研究发展趋势,以期为豆类球蛋白进一步研究及应用提供参考。

  1. Rebalance between 7S and 11S globulins in soybean seeds of differing protein content and 11SA4.

    Science.gov (United States)

    Yang, A; Yu, X; Zheng, A; James, A T

    2016-11-01

    Protein content and globulin subunit composition of soybean seeds affect the quality of soy foods. In this proteomic study, the protein profile of soybean seeds with high (∼45.5%) or low (∼38.6%) protein content and with or without the glycinin (11S) subunit 11SA4 was examined. 44 unique proteins and their homologues were identified and showed that both protein content and 11SA4 influenced the abundance of a number of proteins. The absence of 11SA4 exerted a greater impact than the protein content, and led to a decreased abundance of glycinin G2/A2B1 and G5/A5A4B3 subunits, which resulted in lower total 11S with a concomitant higher total β-conglycinin (7S). Low protein content was associated with higher glycinin G3/A1aB1b and lower glycinin G4/A5A4B3. Using the proteomic approach, it was demonstrated that 11SA4 deficiency induced compensatory accumulation of 7S globulins and led to a similar total abundance for 7S+11S irrespective of protein content or 11SA4. PMID:27211633

  2. Genetic evidence that raised sex hormone binding globulin (SHBG) levels reduce the risk of type 2 diabetes

    DEFF Research Database (Denmark)

    Perry, John R B; Weedon, Michael N; Langenberg, Claudia;

    2010-01-01

    Epidemiological studies consistently show that circulating sex hormone binding globulin (SHBG) levels are lower in type 2 diabetes patients than non-diabetic individuals, but the causal nature of this association is controversial. Genetic studies can help dissect causal directions of epidemiologi...... with diabetes-related intermediate traits, including several measures of insulin secretion and resistance. Our results, together with those from another recent genetic study, strengthen evidence that SHBG and sex hormones are involved in the aetiology of type 2 diabetes.......Epidemiological studies consistently show that circulating sex hormone binding globulin (SHBG) levels are lower in type 2 diabetes patients than non-diabetic individuals, but the causal nature of this association is controversial. Genetic studies can help dissect causal directions...... of epidemiological associations because genotypes are much less likely to be confounded, biased or influenced by disease processes. Using this Mendelian randomization principle, we selected a common single nucleotide polymorphism (SNP) near the SHBG gene, rs1799941, that is strongly associated with SHBG levels. We...

  3. Construction and Analysis of Three-dimensional Graphic Model of Single-chain Fv Derived from an Anti-human Placental Acidic Isoferritin Monoclonal Antibody by Computer

    Institute of Scientific and Technical Information of China (English)

    ZHOU Chun; SHEN Guanxin; ZHU Huifen; YANG Jing; ZHANG Yue; FENG Jiannan; SHEN Beifen

    2000-01-01

    A three-dimensional (3D) graphic model of a single-chain Fv (scFv) which was derived from an anti-human placental acidic isoferritin (PAF) monoclonal antibody (Mab) was constructed by a homologous protein-predicting computer algorithm on Silicon graphic computer station.The structure, surface static electricity and hydrophobicity of scFv were investigated. Computer graphic modelling indicated that all regions of scFv including the linker, variable regions of the heavy (VH) and light (VL) chains were suitable. The VH region and the VL region were involved in composing the "hydrophobic pocket". The linker was drifted away VH and VL regions. The complementarity determining regions (CDRs) of VH and VL regions surrounded the "hydrophobic pocket". This study provides a theory basis for improving antibody affinity, investigating antibody structure and analyzing the functions of VH and VL regions in antibody activity.

  4. Isoforms of thyroxine-binding globulin as a model for molecular epidemiology of human cancer risk

    International Nuclear Information System (INIS)

    -binding globulin (TBG) by 50-60%, but no elevation in free T4, as compared with similar control groups of from Minsk. One of the reasons for the euthyroid syndrome in teenagers from the contaminated region was shown to be the blood isoforms in TBG. These isoforms differed in the structure of the carbohydrate components and as a rule are characterized by a prolonged circulation time. Using specific affinity chromatography, with subsequent immunological assay we shown that a certain amount of serum TBG-molecules in persons from Khojniki contains a high level of TBG (about 30% higher than the control group) containing fucosyalated biantennary sugar chains with more prolonged survival time. Previous findings showed that increased levels of fucosylation are observed for some serum glycoproteins in the blood of tumour patients. We provide evidence for variations in the TBGfuc which are contained in the serum TBG pool of patients suffering from cancer with various localisation. Thus, analysis of TBGfuc may be regarded as a prognostic marker in the determination of risk groups. The molecular mechanism of increased activity of fucosyl transferases will be discussed. We propose to measure the level of TBG fucosylated to assess the human cancer risk and habitants of contaminated region of Belarus. (authors)

  5. A portion of heifers attaining “early puberty” do not display estrus, are anovulatory and have altered sex hormone binding globulin concentrations

    Science.gov (United States)

    Cows with excess androstenedione (High A4) in the follicular fluid of dominant follicles attain puberty earlier than their low androstenedione counterparts. Furthermore, High A4 cows are anovulatory (chronic or sporadic) and have lower Sex Hormone Binding Globulin (SHBG) compared to Low A4 ovulator...

  6. Effects of intraperitoneal insulin versus subcutaneous insulin administration on sex hormone-binding globulin concentrations in patients with type 1 diabetes mellitus

    NARCIS (Netherlands)

    Boering, M; van Dijk, P R; Logtenberg, S J J; Groenier, K H; Wolffenbuttel, B H R; Gans, R O B; Kleefstra, N; Bilo, H J G

    2016-01-01

    AIMS: Elevated sex hormone-binding globulin (SHBG) concentrations have been described in patients with type 1 diabetes mellitus (T1DM), probably due to low portal insulin concentrations. We aimed to investigate whether the route of insulin administration, continuous intraperitoneal insulin infusion

  7. A genome-wide association meta-analysis of circulating sex hormone-binding globulin reveals multiple loci implicated in sex steroid hormone regulation

    NARCIS (Netherlands)

    A.D. Coviello (Andrea); R. Haring (Robin); M. Wellons (Melissa); D. Vaidya (Dhananjay); T. Lehtimäki (Terho); S. Keildson (Sarah); K.L. Lunetta (Kathryn); C. He (Chunyan); M. Fornage (Myriam); V. Lagou (Vasiliki); M. Mangino (Massimo); N.C. Onland-Moret (Charlotte); B. Chen (Benjamin); J. Eriksson (Joel); M. Garcia (Melissa); Y. Liu (Yongmei); A. Koster (Annemarie); K. Lohman (Kurt); L.-P. Lyytikäinen; A.K. Petersen; C.A.J. Prescott; L. Stolk (Lisette); L. Vandenput (Liesbeth); A.R. Wood (Andrew); W.V. Zhuang; A. Ruokonen (Aimo); A.L. Hartikainen; A. Pouta (Anneli); S. Bandinelli (Stefania); R. Biffar (Reiner); G. Brabant (Georg); D.G. Cox (David); S. Cummings; L. Ferrucci (Luigi); M.J. Gunter (Marc J.); S.E. Hankinson (Susan); H. Martikainen (Hannu); A. Hofman (Albert); G. Homuth (Georg); T. Illig (Thomas); J.O. Jansson; A.D. Johnson (Andrew); D. Karasik (David); M. Karlsson (Magnus); J. Kettunen (Johannes); D.P. Kiel (Douglas); P. Kraft (Peter); O.̈. Ljunggren; M. Lorentzon (Mattias); M. Maggio (Marcello); M.R.P. Markus (Marcello R. P.); D. Mellström (Dan); I. Miljkovic (Iva); D. Mirel (Daniel); S. Nelson (Sarah); L. Morin Papunen (Laure); P.H.M. Peeters; I. Prokopenko (Inga); L.J. Raffel (Leslie); M. Reincke (Martin); A.P. Reiner (Alex); K. Rexrode (Kathryn); F. Rivadeneira Ramirez (Fernando); S.M. Schwartz (Stephen); D.S. Siscovick (David); N. Soranzo (Nicole); D. Stöckl (Doris); S. Tworoger (Shelley); A.G. Uitterlinden (André); C.H. van Gils (Carla); R.S. Vasan (Ramachandran Srini); H.E. Wichmann (Erich); G. Zhai (Guangju); S. Bhasin (Shalender); M. Bidlingmaier; S.J. Chanock (Stephen); I. de Vivo (Immaculata); T.B. Harris (Tamara); D. Hunter (David); M. Kähönen (Mika); P. Ouyang (Pamela); T.D. Spector (Timothy); Y.T. van der Schouw (Yvonne); J. Viikari (Jorma); H. Wallaschofski (Henri); M.I. McCarthy (Mark); T.M. Frayling (Timothy); J.C. Murray (Jeffrey); S. Franks (Steve); M.R. Jarvelin; F.A. de Jong (Floris); O. Raitakari (Olli); A. Teumer (Alexander); C. Ohlsson (Claes); J. Murabito (Joanne); J.R.B. Perry (John)

    2012-01-01

    textabstractSex hormone-binding globulin (SHBG) is a glycoprotein responsible for the transport and biologic availability of sex steroid hormones, primarily testosterone and estradiol. SHBG has been associated with chronic diseases including type 2 diabetes (T2D) and with hormone-sensitive cancers s

  8. Effects of a major androgen-dependent urinary protein,. alpha. 2u-globulin on the pituitary-gonadal axis and hypothalamic monoamines in adult male mice

    Energy Technology Data Exchange (ETDEWEB)

    Ghosh, P.K.; Chandrashekar, V.; Steger, R. Bartke, A. (Southern Illinois Univ., Carbondale (USA))

    1990-01-01

    The purpose of the present study was to evaluate the effects of alpha-2u-globulin, a sex-dependent male rat urinary protein on pituitary-gonadal functions and hypothalamic monamine contents in male mice. Adult male mice, maintained under standardized laboratory conditions were injected subcutaneously with alpha-2u-globulin or with vehicle daily for 14 days and killed 16 h after the last injection. Plasma levels of luteinizing hormone (LH), follicle-stimulating hormone (FSH), testosterone (T) and testicular levels of T were measured by radioimmunoassays. The concentrations of norepinephrine (NE), dopamine (DA) and serotonin (5-HT) in medial basal hypothalamus (MBH) and anterior hypothalamus (AH) were measured by high performance liquid chromatography. Administration of alpha-2u-globulin led to a significant increase in plasma FSH and LH levels. In the MBH of alpha-2u-globulin treated mice, there were significant elevations of NE, DA and 5-HT contents. In the AH, both DA and 5-HT contents were decreased while NE content remained unaltered.

  9. Addition of an Enzymatic Hydrolysate of Bovine Globulins to Bread and Determination of Hypotensive Effects in Spontaneously Hypertensive Rats.

    Science.gov (United States)

    Lafarga, Tomas; Gallagher, Eimear; Aluko, Rotimi E; Auty, Mark A E; Hayes, Maria

    2016-03-01

    The aim of this study was to develop bread containing a papain hydrolysate of bovine α- and β-globulins (GPH) with in vitro and in vivo antihypertensive activities. The physical characteristics of the formulated bread were assessed over a six day period and results suggested that the overall quality and acceptance of bread was not affected by the inclusion of GPH at a concentration of 4% (w/w). Bright field light microscopy and confocal scanning laser microscopy images were used to visualize the main ingredients of the bread. In addition, the antihypertensive activity of the bread was assessed in spontaneously hypertensive rats (SHRs) over a 24 h period where a maximum significant decrease in systolic blood pressure of 36.2 ± 1.9 mmHg was observed 8 h after oral administration. Results demonstrate that the antihypertensive activity of GPH was resistant to the baking process and shows potential for use as a functional antihypertensive ingredient. PMID:26876970

  10. Preparation and development of equine hyperimmune globulin F(ab')2 against severe acute respiratory syndrome coronavirus

    Institute of Scientific and Technical Information of China (English)

    Jia-hai LU; Bing L WONG; Nan-shan ZHONG; Zhong-min GUO; Wen-yu HAN; Guo-ling WANG; Ding-mei ZHANG; Yi-fei WANG; Sheng-yun SUN; Qin-he YANG; Huan-ying ZHENG

    2005-01-01

    Aim: The resurgence of severe acute respiratory syndrome (SARS) is still a threat because the causative agent remaining in animal reservoirs is not fully understood,and sporadic cases continue to be reported. Developing high titers of anti-SARS hyperimmune globulin to provide an alternative pathway for emergent future prevention and treatment of SARS. Methods: SARS coronavirus (CoV)F69 (AY313906)and Z2-Y3 (AY394989) were isolated and identified from 2 different Cantonese onset SARS patients. Immunogen was prepared from SARS-CoV F69 strain. Six health horses were immunized 4 times and serum was collected periodically to measure the profile of specific IgG and neutralizing antibodies using indirect enzyme-linked immunosorbent assay and a microneutralization test. Sera were collected in large amounts at the peak, where IgG was precipitated using ammonium sulphate and subsequently digested with pepsin. The product was then purified using anion-exchange chromatography to obtain F(ab')2 fragments. Results: The specific IgG and neutralizing antibody titers peaked at approximately week 7 after the first immunization, with a maximum value of 1:14210. The sera collected at the peak were then purified. Fragment of approximately 15 g F(ab')2 was obtained from 1 litre antiserum and the purity was above 90% with the titer of 1:5120, which could neutralize the other strain (SARS-CoV Z2-Y3) as well. Conclusion: This research provides a viable strategy for the prevention and treatment of SARS coronavirus infection with equine hyperimmune globulin, with the purpose of combating any resurgence of SARS.

  11. Study of chronic hemolytic anaemia patients in Rio de Janeiro: prevalence of anti-human parvovirus B19 IgG antibodies and the developement aplastic crises

    Directory of Open Access Journals (Sweden)

    SANT'ANNA Anadayr L.M.

    2002-01-01

    Full Text Available The prevalence of anti-human parvovirus B19 IgG antibodies was determined in sera from 165 chronic hemolytic anemia patients, receiving medical care at Instituto Estadual de Hematologia (IEHE, Rio de Janeiro, during the year of 1994. This sample represents around 10% of the chronic hemolytic anemia patients attending at IEHE. Most of these patients (140 have sickle cell disease. Anti-B19 IgG antibodies were detected in 32.1% of patients. No statistically significant difference (p > 0.05 was seen between IgG antibody prevalence in male (27.8% and female (35.5% patients. Anti-B19 IgG antibodies were more frequent in older (37.6% than younger (28.2% than 20 years old patients, although this difference had no statistical significance (p > 0.05. Anti-B19 IgG antibody prevalence showed that 67.9% of patients enrolled in the study were susceptible to B19 acute infection. With the aim to detect acute B19 infection, patients follow up continued until February 1996. During this period four patients presented transient aplastic crisis due to human parvovirus B19 as confirmed by the detection of specific IgM antibodies. All four patients were younger than 20 years old, and 3 were younger than 10 years old. Three of them were sickle cell disease patients. Three of the four acute B19 infection occurred during 1994 springtime.

  12. Preparation and Characterization of Covalently Binding of Rat Anti-human IgG Monolayer on Thiol-Modified Gold Surface

    Directory of Open Access Journals (Sweden)

    Lv Zhengjian

    2009-01-01

    Full Text Available Abstract The 16-mercaptohexadecanoic acid (MHA film and rat anti-human IgG protein monolayer were fabricated on gold substrates using self-assembled monolayer (SAM method. The surface properties of the bare gold substrate, the MHA film and the protein monolayer were characterized by contact angle measurements, atomic force microscopy (AFM, grazing incidence X-ray diffraction (GIXRD method and X-ray photoelectron spectroscopy, respectively. The contact angles of the MHA film and the protein monolayer were 18° and 12°, respectively, all being hydrophilic. AFM images show dissimilar topographic nanostructures between different surfaces, and the thickness of the MHA film and the protein monolayer was estimated to be 1.51 and 5.53 nm, respectively. The GIXRD 2θ degrees of the MHA film and the protein monolayer ranged from 0° to 15°, significantly smaller than that of the bare gold surface, but the MHA film and the protein monolayer displayed very different profiles and distributions of their diffraction peaks. Moreover, the spectra of binding energy measured from these different surfaces could be well fitted with either Au4f, S2p or N1s, respectively. Taken together, these results indicate that MHA film and protein monolayer were successfully formed with homogeneous surfaces, and thus demonstrate that the SAM method is a reliable technique for fabricating protein monolayer.

  13. Biodistribution and γ imaging of 125I-labeled goat anti-human IgG polyclonal antibody in nude mice bearing human colon cancer xenografts

    International Nuclear Information System (INIS)

    The possibility of IgG secreted from tumor cells as a target for radioimmunoimaging and targeted therapy of cancers were investigated. Goat anti-human IgG polyclonal anti- body (GAHG) was radioiodinated using Iodogen method, and the in vitro stability and pharmacokinetics were evaluated. The biodistribution and γ imaging of 125I-GAHG were performed in nude mice bearing HT-29 human colon cancer xenografts. 125I-GAHG showed good in vitro stability, and its blood clearance was defined as a two-compartment model, with T1/2α and T1/2β were 1.19 h and 43.99 h, respectively. The tumor uptake of 125I-GAHG was higher than that of 125I-labeled normal goat IgG control (125I-GIgG). 125I-GAHG showed good tumor retention when injecting via intra-tumor. In the biodistribution study, the highest tumor uptake of 125I-GAHG was 6.71±2.19%ID/g at 72 h postinjection and the T/NT increased along with the postinjection time. The results show that 125I-GAHG have good tumor-specific uptake which may provide a novel idea for radioimmunoimaging and targeted therapy of cancers. (authors)

  14. The 11S globulin Sin a 2 from yellow mustard seeds shows IgE cross-reactivity with homologous counterparts from tree nuts and peanut

    Directory of Open Access Journals (Sweden)

    Sirvent Sofía

    2012-12-01

    Full Text Available Abstract Background The 11S globulin Sin a 2 is a marker to predict severity of symptoms in mustard allergic patients. The potential implication of Sin a 2 in cross-reactivity with tree nuts and peanut has not been investigated so far. In this work, we studied at the IgG and IgE level the involvement of the 11S globulin Sin a 2 in cross-reactivity among mustard, tree nuts and peanut. Methods Eleven well-characterized mustard-allergic patients sensitized to Sin a 2 were included in the study. A specific anti-Sin a 2 serum was obtained in rabbit. Skin prick tests (SPT, enzyme-linked immunosorbent assay (ELISA, immunoblotting and IgG or IgE-inhibition immunoblotting experiments using purified Sin a 2, Sin a 1, Sin a 3, mustard, almond, hazelnut, pistachio, walnut or peanut extracts were performed. Results The rabbit anti-Sin a 2 serum showed high affinity and specificity to Sin a 2, which allowed us to demonstrate that Sin a 2 shares IgG epitopes with allergenic 11S globulins from tree nuts (almond, hazelnut, pistachio and walnut but not from peanut. All the patients included in the study had positive skin prick test to tree nuts and/or peanut and we subdivided them into two different groups according to their clinical symptoms after ingestion of such allergenic sources. We showed that 11S globulins contain conserved IgE epitopes involved in cross-reactivity among mustard, tree nuts and peanut as well as species-specific IgE epitopes. Conclusions The allergenic 11S globulin Sin a 2 from mustard is involved in cross-reactivity at the IgE level with tree nuts and peanut. Although the clinical relevance of the cross-reactive IgE epitopes present in 11S globulins needs to be investigated in further detail, our results contribute to improve the diagnosis and management of mustard allergic patients sensitized to Sin a 2.

  15. Intravenous immune globulin (10% caprylate-chromatography purified) for the treatment of chronic inflammatory demyelinating polyradiculoneuropathy (ICE study): a randomised placebo-controlled trial

    OpenAIRE

    Hughes, Richard; Donofrio, Peter; Bril, Vera; Dalakas, Marinos; Deng, Chunqin; Hanna, Kim; Hartung, H. P.; Latov, Norman; Merkies, Ingemar; Doorn, Pieter

    2008-01-01

    textabstractBackground: Short-term studies suggest that intravenous immunoglobulin might reduce disability caused by chronic inflammatory demyelinating polyradiculoneuropathy (CIDP) but long-term effects have not been shown. We aimed to establish whether 10% caprylate-chromatography purified immune globulin intravenous (IGIV-C) has short-term and long-term benefit in patients with CIDP. Methods: 117 patients with CIDP who met specific neurophysiological inflammatory neuropathy cause and treat...

  16. Sensitization with 7S Globulins from Peanut, Hazelnut, Soy or Pea Induces IgE with Different Biological Activities Which Are Modified by Soy Tolerance

    DEFF Research Database (Denmark)

    Kroghsbo, Stine; Bøgh, Katrine Lindholm; Rigby, Neil M.;

    2011-01-01

    , such as stability to digestion, have also been suggested. 7S globulins from peanut, hazelnut, soy, and pea were studied to determine whether related proteins would induce a similar sensitization when removed from their ‘normal’ matrix. Methods: Brown Norway rats (soy tolerant or nontolerant) were immunized i.p. 3...... times with 100 μg purified peanut, hazelnut, soy, or pea 7S without adjuvant. Sera were analyzed for specific antibodies by different ELISAs (IgG1, IgG2a, and IgE), inhibition ELISA, and rat basophilic leukemia cell assay. Results: The 4 related 7S globulins induced a response with an almost identical...... level of specific antibodies, but peanut 7S induced IgE of higher avidity than hazelnut and pea 7S which, again, had a higher avidity than IgE induced by soy 7S. Soy tolerance reduced the functionality of IgE without influencing antibody titers. Conclusions: Although the 4 7S globulins are structurally...

  17. A BIOCHEMICAL STUDY OF THE PHENOMENA KNOWN AS COMPLEMENT-SPLITTING : FIRST PAPER: SPLITTING OF THE COMPLEMENT ASSOCIATED WITH GLOBULIN PRECIPITATION.

    Science.gov (United States)

    Bronfenbrenner, J; Noguchi, H

    1912-06-01

    It is generally accepted that complement may be split into a mid-piece and an end-piece. The mid-piece is thought to be in the globulin fraction, and the end-piece in the albumin fraction. The restoration of complement activity by putting together the albumin and globulin fractions does not prove, however, that each fraction contained a part of the complement, for the albumin fraction can be reactivated in the absence of the globulin fraction. Complement-splitting as brought about by hydrochloric acid, carbon dioxid, and dialysis, is really an inactivation of the whole complement by certain acids or alkalis, either added in the free state to the serum, or liberated as a result of the dissociation of certain electrolytes. That the whole complement, and not a part only, is present in the albumin fraction of the serum can be demonstrated by the removal of the inhibitory action of the acid or alkali. This can be effected by the addition, not only of alkali or acid, but also of any amphoteric substance. When hydrochloric acid, carbon dioxid, or dialysis are employed to produce the phenomenon known as complement-splitting, the complement is merely inactivated, not split.

  18. Successful prevention of post-transfusion Rh alloimmunization by intravenous Rho (D) immune globulin (WinRho SD).

    Science.gov (United States)

    Anderson, B; Shad, A T; Gootenberg, J E; Sandler, S G

    1999-03-01

    Alloimmunization to the D blood group antigen following the transfusion of D-positive red blood cells to a D-negative recipient may be prevented in most persons by a prompt and adequate dose of Rho (D) immune globulin (RhIG). Until recently, the only RhIG approved by the US Food and Drug Administration (FDA) for this indication required intramuscular injection, an inconvenient and painful route for the relatively large volume that may be required. We describe the successful prevention of Rh alloimmunization following the unintentional transfusion of D-positive red blood cells to a D-negative infant by the intravenous infusion of WinRho SD, a new RhIG that is FDA-approved for prevention of post-transfusion Rh alloimmunization by intravenous administration. We believe that this more convenient and less painful approach should be the treatment of choice for preventing Rh alloimmunization following the transfusion of D-positive red cells to a D-negative recipient. PMID:10072121

  19. Heat-denaturation and aggregation of quinoa (Chenopodium quinoa) globulins as affected by the pH value.

    Science.gov (United States)

    Mäkinen, Outi E; Zannini, Emanuele; Koehler, Peter; Arendt, Elke K

    2016-04-01

    The influence of heating (100 °C; 0-15 min) on the relative molecular mass, protein unfolding and secondary structure of quinoa globulins was studied at pH 6.5 (low solubility), 8.5 and 10.5 (high solubility). The patterns of denaturation and aggregation varied with pH. Heating triggered the disruption of the disulfide bonds connecting the acidic and basic chains of the chenopodin subunits at pH 8.5 and 10.5, but not at pH 6.5. Large aggregates unable to enter a 4% SDS-PAGE gel were formed at pH 6.5 and 8.5, which became soluble under reducing conditions. Heating at pH 10.5 lead to a rapid dissociation of the native chenopodin and to the disruption of the subunits, but no SDS-insoluble aggregates were formed. No major changes in secondary structure occurred during a 15 min heating, but an increase in hydrophobicity indicated unfolding of the tertiary structure in all samples. PMID:26593460

  20. Intramuscular hepatitis B immune globulin combined with lamivudine in prevention of hepatitis B recurrence after liver transplantation

    Institute of Scientific and Technical Information of China (English)

    Mao-Lin Yan; Yong-Bing Chen; Lu-Nan Yan; Bo Li; Yong Zeng; Tian-Fu Wen; Wen-Tao Wang; Jia-Yin Yang; Ming-Qing Xu; Zhi-Hui Li

    2006-01-01

    BACKGROUND: Combined hepatitis B immune globulin (HBIg) and lamivudine in prophylaxis of the recurrence of hepatitis B after liver transplantation has signiifcantly improved the survival of HBsAg positive patients. This study was undertaken to evaluate the outcomes of liver transplantation for patients with hepatitis B virus (HBV). METHODS: A retrospective chart analysis and a review of the organ transplant database identiifed 51 patients (43 men and 8 women) transplanted for benign HBV-related cirrhotic diseases between June 2002 and December 2004 who had survived more than 3 months. HBIg was administered intravenously during the ifrst week and intramuscularly thereafter. RESULTS: At a median follow-up of 14.1 months, the overall recurrence rate in the 51 patients was 3.9%(2/51). The overall patient survival was 88.3%, and 82.4%after 1 and 2 years, respectively. A daily oral dose of 100 mg lamivudine for 2 weeks before transplantation for 10 patients enabled 57.1%(4/7) and 62.5%(5/8) of HBV-DNA and HBeAg positive patients respectively to convert to be negative. Intramuscular HBIg was well tolerated in all patients. CONCLUSION:Lamivudine combined with intramuscular HBIg can effectively prevent allograft from the recurrence of HBV after liver transplantation.

  1. Agrobacterium-mediated transformation of the β-subunit gene in 7S globulin protein in soybean using RNAi technology.

    Science.gov (United States)

    Qu, J; Liu, S Y; Wang, P W; Guan, S Y; Fan, Y G; Yao, D; Zhang, L; Dai, J L

    2016-04-26

    The objective of this study was to use RNA interference (RNAi) to improve protein quality and decrease anti-nutritional effects in soybean. Agrobacterium tumefaciens-mediated transformation was conducted using RNAi and an expression vector containing the 7S globulin β-subunit gene. The BAR gene was used as the selective marker and cotyledonary nodes of soybean genotype Jinong 27 were chosen as explant material. Regenerated plants were detected by molecular biology techniques. Transformation of the β-subunit gene in the 7S protein was detected by PCR, Southern blot, and q-PCR. Positive plants (10 T0, and 6 T1, and 13 T2) were tested by PCR. Hybridization bands were detected by Southern blot analysis in two of the T1 transgenic plants. RNAi expression vectors containing the soybean 7S protein β-subunit gene were successfully integrated into the genome of transgenic plants. qRT-PCR analysis in soybean seeds showed a clear decrease in expression of the soybean β-subunit gene. The level of 7S protein β-subunit expression in transgenic plants decreased by 77.5% as compared to that of the wild-type plants. This study has established a basis for the application of RNAi to improve the anti-nutritional effects of soybean.

  2. Expression and characterization of a His-tagged 11S seed globulin from Amaranthus hypochondriacus in Escherichia coli.

    Science.gov (United States)

    Medina-Godoy, Sergio; Nielsen, Niels C; Paredes-López, Octavio

    2004-01-01

    DNA encoding a His-tagged 11S globulin from Amaranthus hypochondriacus (amarantin) was successfully expressed in Escherichia coli strains BL21 (DE3) and Origami (DE3). The two strains produced different accumulation patterns. Whereas most of the proamarantin expressed in BL21 (DE3) was localized in inclusion bodies, that produced in Origami (DE3) was soluble (76 mg/L). Sucrose density gradient ultracentrifugation analysis of the expressed soluble proamarantin revealed that the protein was assembled into trimers. Treatment of proamarantin trimers in vitro using purified asparaginyl endopeptidase resulted in the appearance of peptides of the sizes expected for acidic and basic chains. Because the proamarantin assembles into trimers with the expected sedimentation characteristics and is cleaved into acidic and basic chains rather than being degraded, the results suggest that the protein folding occurring in E. coli is similar to that taking place in seeds. The His-tagged proamarantin was purified in a single step by immobilized metal affinity chromatography with a final yield of 48 mg/L. The overexpression of proamarantin in E. coli, together with the one-step purification will facilitate further investigation of this storage protein through site-directed mutagenesis.

  3. Anti-Human Tissue Factor Antibody Ameliorated Intestinal Ischemia Reperfusion-Induced Acute Lung Injury in Human Tissue Factor Knock-In Mice

    Science.gov (United States)

    Mura, Marco; Li, Li; Cypel, Marcelo; Soderman, Avery; Picha, Kristen; Yang, Jing; Liu, Mingyao

    2008-01-01

    Background Interaction between the coagulation and inflammation systems plays an important role in the development of acute respiratory distress syndrome (ARDS). Anti-coagulation is an attractive option for ARDS treatment, and this has promoted development of new antibodies. However, preclinical trials for these antibodies are often limited by the high cost and availability of non-human primates. In the present study, we developed a novel alternative method to test the role of a humanized anti-tissue factor mAb in acute lung injury with transgenic mice. Methodology/Principal Findings Human tissue factor knock-in (hTF-KI) transgenic mice and a novel humanized anti-human tissue factor mAb (anti-hTF mAb, CNTO859) were developed. The hTF-KI mice showed a normal and functional expression of hTF. The anti-hTF mAb specifically blocked the pro-coagulation activity of brain extracts from the hTF-KI mice and human, but not from wild type mice. An extrapulmonary ARDS model was used by intestinal ischemia-reperfusion. Significant lung tissue damage in hTF-KI mice was observed after 2 h reperfusion. Administration of CNTO859 (5 mg/kg, i.v.) attenuated the severity of lung tissue injury, decreased the total cell counts and protein concentration in bronchoalveolar lavage fluid, and reduced Evans blue leakage. In addition, the treatment significantly reduced alveolar fibrin deposition, and decreased tissue factor and plasminogen activator inhibitor-1 activity in the serum. This treatment also down-regulated cytokine expression and reduced cell death in the lung. Conclusions This novel anti-hTF antibody showed beneficial effects on intestinal ischemia-reperfusion induced acute lung injury, which merits further investigation for clinical usage. In addition, the use of knock-in transgenic mice to test the efficacy of antibodies against human-specific proteins is a novel strategy for preclinical studies. PMID:18231608

  4. Cytotoxicity of an ebulin l-anti-human CD105 immunotoxin on mouse fibroblasts (L929) and rat myoblasts (L6E9) cells expressing human CD105.

    Science.gov (United States)

    Benítez, Jorge; Ferreras, J Miguel; Muñoz, Raquel; Arias, Yolanda; Iglesias, Rosario; Córdoba-Díaz, Manuel; del Villar, Rosario; Girbés, Tomás

    2005-01-01

    Tumour growth is characterised by the formation of a fine vessel network or neovasculature which nourishes tumour cells. Two kinds of novel anti-angiogenic therapies are based on the prevention of vessels growth and on the destruction of those vessels already formed. We report here on the design and construction of a novel immunotoxin formed with the non-toxic type II ribosome-inactivating protein ebulin l and the mouse anti-human CD105 monoclonal antibody 44G4. The 44G4-ebulin immunotoxin was formed by covalent linking of both proteins with N-succinimidyl-3-(2-pyridyldithio)propionate (SPDP) and was purified by chromatography on Superdex 200 HiLoad. The analysis of the anti-ribosomal effects in a cell-free translation system indicated that conjugation does not affect the activity of ebulin l. The immunotoxin displays cytotoxicity with nanomolar IC50 values on human CD105+ cells like the mouse fibroblasts L929 cells transfected with the short form of human CD105 and the rat myoblasts L6E9 transfected with the long form of human CD105. In contrast, cells lacking human CD105 were 2-2.5 logs less sensitive to the immunotoxin. Free ebulin displays IC50 values in the range 10(-6) M. Since CD105 is being considered as a potential target for the anti-vascular therapy of tumours, the present immunotoxin could be a promising tool for the anticancer therapy, especially due to the very low in vivo toxicity of ebulin l as compared ricin and other toxins used for immunotoxins.

  5. Radiolabeling of anti-human prostatic specific membrane antigen antibody with 99Tcm and its biodistribution in nude mice bearing human prostate cancer

    International Nuclear Information System (INIS)

    Objective: To study the binding affinity of 99Tcm labeled anti-human prostatic specific membrane antigen (PSMA) monoclonal antibody (McAb) J591 to prostate cancer cells and the biodistribution of 99Tcm-J591 in nude mice bearing human prostate cancer. Methods: The McAb J591 was labeled with vTcm by improved Schwarz method and the labeled McAb was purified by Sephadex G-50. The binding affinity of J591 with prostate cancer cells was measured by Flow Cytometry. The nude mice bearing PSMA-positive C4-2 prostate carcinoma xenografts were served as experiment groups, mice with PSMA-negative pc3 tumors served as controls. The biodistribution of 99Tcm-J591 were carried out in both model nude mice. Results: The radiolabeling efficiency of 99Tcm-J591 was 78.9±6.2%, and radiochemical purity was more than 90% after purification. The 99Tcm-J591 showed a good combination with PSMA-positive C4-2 cells and no combination with PSMA-negative PC3 cells in vitro. The biodistribution results showed that 99Tcm-J591 was accumulated in tumor tissue during the 2-24 hours after injection in experiment groups, and no significant uptake in control group. The uptake of 99Tcm-J591 in tumor tissue reached a maximum 15.91±5.16 % ID/g in experimental group at 12h post-injection. There was a significant difference compared with controls (P0.05). Conclusion: The monoclonal antibody J591 exhibits an excellent immuno-reactivity and tumor targeting property, and it may be used in diagnosis and target therapy of prostate cancer. (authors)

  6. Pharmacokinetics, biodistribution and dosimetry of 99mTc-labeled anti-human epidermal growth factor receptor humanized monoclonal antibody R3 in rats.

    Science.gov (United States)

    Iznaga Escobar, N; Morales, A M; Ducongé, J; Torres, I C; Fernández, E; Gómez, J A

    1998-01-01

    The pharmacokinetics, biodistribution and dosimetry of 99mTc-labeled anti-human epidermal growth factor receptor (anti-hEGF-r) humanized monoclonal antibody (MAb) R3 was investigated following intravenous injection in normal Wistar rats. Serum disappearance curves were best fit by a two-compartment model having a mean distribution half-life (t 1/2alpha) of 0.250 h and a mean elimination (t 1/2beta) of 13.89 h. Among the various organs, a little accumulation of the radiolabeled antibody was found only in kidneys. Biodistribution and dosimetry studies in humans were performed by extrapolation of the animal data to humans. Absorbed dose to normal organs and the remainder of the whole body were estimated using the medical internal radiation dose formula, and dose contributions from radioactivity in transit through the gastrointestinal tract were estimated using a compartment model. Extrapolated values of radiation absorbed dose to normal organs in rads per millicurie administered were whole body, 0.0085; lower large intestine wall, 0.0898; small intestine, 0.0530; upper large intestine wall, 0.0731; and kidneys, 0.0455. The effective dose equivalent predicted was 0.0162 rem/mCi and the effective dose was found to be 0.015 rem/mCi. On the basis of the pharmacokinetics, biodistribution and internal radiation dosimetry information obtained in this study, a diagnostic phase I clinical trial with 99mTc-labeled humanized MAb R3 conjugate in patients should be supported.

  7. Induction of Broad and Potent Anti-Human Immunodeficiency Virus Immune Responses in Rhesus Macaques by Priming with a DNA Vaccine and Boosting with Protein-Adsorbed Polylactide Coglycolide Microparticles

    OpenAIRE

    Otten, Gillis; Schaefer, Mary; Greer, Catherine; Calderon-Cacia, Maria; Coit, Doris; Kazzaz, Jina; Medina-Selby, Angelica; Selby, Mark; Singh, Manmohan; Ugozzoli, Mildred; zur Megede, Jan; Barnett, Susan W.; O'Hagan, Derek; Donnelly, John; Ulmer, Jeffrey

    2003-01-01

    Several vaccine technologies were evaluated for their abilities to induce anti-human immunodeficiency virus Gag immune responses in rhesus macaques. While no vaccine alone was able to induce broad and strong immune responses, these were achieved by priming with Gag DNA and boosting with Gag protein adsorbed to polylactide coglycolide microparticles. This regimen elicited strong antibodies, helper T cells, and cytotoxic T lymphocytes and thus holds promise as an effective vaccination scheme.

  8. Hyperimmune globulins and same-day thrombotic adverse events as recorded in a large healthcare database during 2008-2011.

    Science.gov (United States)

    Menis, Mikhail; Sridhar, Gayathri; Selvam, Nandini; Ovanesov, Mikhail V; Divan, Hozefa A; Liang, Yideng; Scott, Dorothy; Golding, Basil; Forshee, Richard; Ball, Robert; Anderson, Steven A; Izurieta, Hector S

    2013-12-01

    Thrombotic events (TEs) are rare serious complications following administration of hyperimmune globulin (HIG) products. Our retrospective claims-based study assessed occurrence of same-day TEs following administration of HIGs during 2008-2011 and examined potential risk factors using HealthCore's Integrated Research Database (HIRD(SM) ) and laboratory testing of products' procoagulant Factor XIa activity by U.S. Food and Drug Administration. Multivariable regression was used to estimate same-day TE risk for different products. Of 101,956 individuals exposed to 23 different HIG product groups, 86 (0.84 per 1,000 persons) had a TE diagnosis code (DC) recorded on the same day as HIG administration. Unadjusted same-day TE DC rates (per 1,000 persons) ranged from 0.4 to 148.9 for different products. GamaSTAN S/D IG >10 cc had statistically significantly higher same-day TE DC risk compared to Tetanus IG (OR = 57.57; 95% CI = 19.72-168.10). Increased TE risk was also observed with older age (≥45 years), prior thrombotic events, and hypercoagulable state(s). Laboratory investigation identified elevated Factor XIa activity for GamaSTAN S/D, HepaGam B, HyperHep B S/D, WinRho SDF, HyperRHO S/D full dose, and HyperTET S/D. Our study, for the first time, identified increase in the same-day TE DC risk with GamaSTAN S/D IG >10 cc and suggests potentially elevated TE risk with other HIGs. PMID:23907744

  9. Long-term outcome of 25 children and adolescents with severe aplastic anemia treated with antithymocyte globulin

    Directory of Open Access Journals (Sweden)

    de-Medeiros C.R.

    2000-01-01

    Full Text Available Severe aplastic anemia (SAA is probably an immune-mediated disorder, and immunosuppressive therapy is recommended for patients with no available donor for bone marrow transplant. Between October 1984 and November 1987, 25 consecutive children and adolescents with SAA with no HLA-compatible marrow donor received equine antithymocyte globulin (ATG (15 mg kg-1 day-1 for 10 days. The patients were evaluated 6 weeks, 6 months, and 12 months after starting ATG treatment. Thereafter, patients were evaluated yearly until July 1998. Median age was 10 years (range, 1.5-20 years, granulocyte counts on referral ranged from 0.032 to 1.4 x 10(9/l (median 0.256 x 10(9/l, and 12 patients had granulocyte counts <0.2 x 10(9/l. At a median follow-up of 9.6 years (range, 8.6-11.8 years, 10 patients (40% remained alive with good marrow function. No morphologic evidence of hematological clonal disorders has been observed, although two patients probably have acquired clonal chromosomal abnormalities (trisomy 8 and del(6q21, respectively. Responses to ATG were observed between 6 weeks and 6 months from the start of treatment in 60% of evaluable patients. The response rate was not different in patients whose granulocyte count at diagnosis was <0.2 x 10(9/l, or in those who were <10 years of age. This study supports the view that, when compared with supportive measures, ATG is an effective treatment for children or adolescents with SAA. Although these results are inferior to those reported for marrow transplantation or more intensive immunosuppressive regimens, these patients who responded to ATG are long-term survivors with stable peripheral blood counts and a low rate of relapse.

  10. New directions for rabbit antithymocyte globulin (Thymoglobulin(®)) in solid organ transplants, stem cell transplants and autoimmunity.

    Science.gov (United States)

    Mohty, Mohamad; Bacigalupo, Andrea; Saliba, Faouzi; Zuckermann, Andreas; Morelon, Emmanuel; Lebranchu, Yvon

    2014-09-01

    In the 30 years since the rabbit antithymocyte globulin (rATG) Thymoglobulin(®) was first licensed, its use in solid organ transplantation and hematology has expanded progressively. Although the evidence base is incomplete, specific roles for rATG in organ transplant recipients using contemporary dosing strategies are now relatively well-identified. The addition of rATG induction to a standard triple or dual regimen reduces acute cellular rejection, and possibly humoral rejection. It is an appropriate first choice in patients with moderate or high immunological risk, and may be used in low-risk patients receiving a calcineurin inhibitor (CNI)-sparing regimen from time of transplant, or if early steroid withdrawal is planned. Kidney transplant patients at risk of delayed graft function may also benefit from the use of rATG to facilitate delayed CNI introduction. In hematopoietic stem cell transplantation, rATG has become an important component of conventional myeloablative conditioning regimens, following demonstration of reduced acute and chronic graft-versus-host disease. More recently, a role for rATG has also been established in reduced-intensity conditioning regimens. In autoimmunity, rATG contributes to the treatment of severe aplastic anemia, and has been incorporated in autograft projects for the management of conditions such as multiple sclerosis, Crohn's disease, and systemic sclerosis. Finally, research is underway for the induction of tolerance exploiting the ability of rATG to induce immunosuppresive cells such as regulatory T-cells. Despite its long history, rATG remains a key component of the immunosuppressive armamentarium, and its complex immunological properties indicate that its use will expand to a wider range of disease conditions in the future.

  11. Genome wide association identifies common variants at the SERPINA6/SERPINA1 locus influencing plasma cortisol and corticosteroid binding globulin.

    Directory of Open Access Journals (Sweden)

    Jennifer L Bolton

    2014-07-01

    Full Text Available Variation in plasma levels of cortisol, an essential hormone in the stress response, is associated in population-based studies with cardio-metabolic, inflammatory and neuro-cognitive traits and diseases. Heritability of plasma cortisol is estimated at 30-60% but no common genetic contribution has been identified. The CORtisol NETwork (CORNET consortium undertook genome wide association meta-analysis for plasma cortisol in 12,597 Caucasian participants, replicated in 2,795 participants. The results indicate that <1% of variance in plasma cortisol is accounted for by genetic variation in a single region of chromosome 14. This locus spans SERPINA6, encoding corticosteroid binding globulin (CBG, the major cortisol-binding protein in plasma, and SERPINA1, encoding α1-antitrypsin (which inhibits cleavage of the reactive centre loop that releases cortisol from CBG. Three partially independent signals were identified within the region, represented by common SNPs; detailed biochemical investigation in a nested sub-cohort showed all these SNPs were associated with variation in total cortisol binding activity in plasma, but some variants influenced total CBG concentrations while the top hit (rs12589136 influenced the immunoreactivity of the reactive centre loop of CBG. Exome chip and 1000 Genomes imputation analysis of this locus in the CROATIA-Korcula cohort identified missense mutations in SERPINA6 and SERPINA1 that did not account for the effects of common variants. These findings reveal a novel common genetic source of variation in binding of cortisol by CBG, and reinforce the key role of CBG in determining plasma cortisol levels. In turn this genetic variation may contribute to cortisol-associated degenerative diseases.

  12. A retrospective comparison of cyclophosphamide plus antithymocyte globulin with cyclophosphamide plus busulfan as the conditioning regimen for severe aplastic anemia

    Directory of Open Access Journals (Sweden)

    L.V.M. Ommati

    2009-03-01

    Full Text Available Allogeneic hematopoietic stem cell transplantation (AHSCT is the treatment of choice for young patients with severe aplastic anemia (SAA. The association of antithymocyte globulin (ATG and cyclophosphamide (CY is the most frequently used conditioning regimen for this disease. We performed this retrospective study in order to compare the outcomes of HLA-matched sibling donor AHSCT in 41 patients with SAA receiving cyclophosphamide plus ATG (ATG-CY, N = 17 or cyclophosphamide plus busulfan (BU-CY, N = 24. The substitution of BU for ATG was motivated by the high cost of ATG. There were no differences in the clinical features between the two groups, including age, gender, cytomegalovirus status, ABO match, interval between diagnosis and transplant, and number of total nucleated cells infused. No differences were observed in the time to neutrophil and platelet engraftment, or in the risk of veno-occlusive disease and hemorrhage. However, there was a higher risk of mucositis in the BU-CY group (71 vs 24%, P = 0.004. There were no differences in the incidence of neutrophil and platelet engraftment, acute and chronic graft-versus-host disease, and transplant-related mortality. There was a higher incidence of late rejection in the ATG-CY group (41 vs 4%, P = 0.009. Although the ATG-CY group had a longer follow-up (101 months than the BU-CY group (67 months, P = 0.04, overall survival was similar between the groups (69 vs 58%, respectively, P = 0.32. We conclude that the association BU-CY is a feasible option to the conventional ATG-CY regimen in this population.

  13. Anti-human tissue factor antibody ameliorated intestinal ischemia reperfusion-induced acute lung injury in human tissue factor knock-in mice.

    Directory of Open Access Journals (Sweden)

    Xiaolin He

    Full Text Available BACKGROUND: Interaction between the coagulation and inflammation systems plays an important role in the development of acute respiratory distress syndrome (ARDS. Anti-coagulation is an attractive option for ARDS treatment, and this has promoted development of new antibodies. However, preclinical trials for these antibodies are often limited by the high cost and availability of non-human primates. In the present study, we developed a novel alternative method to test the role of a humanized anti-tissue factor mAb in acute lung injury with transgenic mice. METHODOLOGY/PRINCIPAL FINDINGS: Human tissue factor knock-in (hTF-KI transgenic mice and a novel humanized anti-human tissue factor mAb (anti-hTF mAb, CNTO859 were developed. The hTF-KI mice showed a normal and functional expression of hTF. The anti-hTF mAb specifically blocked the pro-coagulation activity of brain extracts from the hTF-KI mice and human, but not from wild type mice. An extrapulmonary ARDS model was used by intestinal ischemia-reperfusion. Significant lung tissue damage in hTF-KI mice was observed after 2 h reperfusion. Administration of CNTO859 (5 mg/kg, i.v. attenuated the severity of lung tissue injury, decreased the total cell counts and protein concentration in bronchoalveolar lavage fluid, and reduced Evans blue leakage. In addition, the treatment significantly reduced alveolar fibrin deposition, and decreased tissue factor and plasminogen activator inhibitor-1 activity in the serum. This treatment also down-regulated cytokine expression and reduced cell death in the lung. CONCLUSIONS: This novel anti-hTF antibody showed beneficial effects on intestinal ischemia-reperfusion induced acute lung injury, which merits further investigation for clinical usage. In addition, the use of knock-in transgenic mice to test the efficacy of antibodies against human-specific proteins is a novel strategy for preclinical studies.

  14. Cytoplasmic androgen binding protein of rat liver: molecular characterization after photoaffinity labeling and functional correlation with the age-dependent synthesis of alpha 2u-globulin

    International Nuclear Information System (INIS)

    The liver of the mature male rat contains a moderate affinity (Kd = 10(-8)M), low-capacity, cytoplasmic androgen binding protein (CAB) whose appearance during puberty and disappearance during senescence correlate with the androgen-dependent synthesis of alpha 2u-globulin. Molecular properties of CAB were examined by photoaffinity labeling with tritiated methyltrienolone (R-1881), a synthetic androgen, and by its localization within the hepatocytes which are competent to produce alpha 2u-globulin. Photoaffinity labeling of the liver cytosol derived from postpubertal male rats, followed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and fluorography, showed a predominant androgen binding band corresponding to Mr 31,000. This 31-kilodalton (kDa) binding component was conspicuously absent in the liver of androgen-insensitive prepubertal and senescent male rats and in adult male rats treated with estradiol-17 beta. In addition, unlike the cytoplasmic extract, the nuclear lysate of the male rat hepatocytes did not contain the 31-kDa androgen binder. Disappearance of the 31-kDa androgen binding band from the cytosolic fraction of androgen-insensitive animals was associated with a concomitant appearance of a minor androgen binding component of apparent Mr 29,000. The livers of postpubertal male rats normally contain two subpopulations of hepatocytes, only one of which is highly active (competent) in alpha 2u-globulin synthesis. Separation of these two subpopulations through a fluorescence-activated cell sorter followed by whole cell labeling showed more than a 2-fold higher uptake of R-1881 by the competent cells

  15. The effect of TACE combined with tumor interstitial therapy on immune globulin, complement and T lymphocyte subsets in patients with liver cancer

    Institute of Scientific and Technical Information of China (English)

    Bu-Tian Li; Sheng-Ping Zhang

    2016-01-01

    Objective:To explore the effect of TACE combined with interstitial therapy on immune globulin, complement and T lymphocyte subsets in patients with liver cancer.Methods:A total of 95 patients with liver cancer from March 2013 to April 2015 in the hospital were selected, and they were randomly divided into group A (n = 47) and group B (n=48), patients in group A were given simple TACE treatment, while patients in group B underwent TACE + tumor interstitial therapy (percutaneous liver tumor injection of lipiodol chemotherapy). Immune globulin, complement and T lymphocyte subsets in peripheral blood preoperative 1w and postoperative 4w were observed.Results: After treatment, the lipiodol deposition rate, local control rate and tumor reduction rate of group B were significantly higher than that in group A, which had statistically significant difference between the two groups. Compared with that before treatment, CD3+, CD4+, CD4+/CD8+ were significantly increased, CD8+ was significantly decreased after treatment. There were significantly differences in CD3+, CD4+, CD4+/CD8+, CD8+ between the two groups after treatment. The immune globulin and complement levels increased in group A after treatment, but had no significant difference. Immunoglobulin and complement levels increased significantly in group B, there were significantly differences in immunoglobulin and complement levels in the two groups after treatment.Conclusions:The results showed that single TACE treatment in patients with liver cancer could enhance immune function, which had little effect on the humoral immunity. TACE combined with interstitial therapy could improve patients' cellular immunity and humoral immunity, which was benefit for prognosis.

  16. Localization of the human thyroxine-binding globulin gene to the long arm of the X chromosome (Xq21-22).

    OpenAIRE

    Trent, J M; Flink, I L; Morkin, E; van Tuinen, P; Ledbetter, D H

    1987-01-01

    Thyroxine-binding globulin (TBG) is the major thyroid-hormone transport protein in the plasma of most vertebrate species. A recombinant phage (lambda cTBG8) containing a cDNA insert of human TBG recently has been described. With the cDNA insert from lambda cTBG8 used as a radiolabeled probe, DNA from a series of somatic-cell hybrids containing deletions of the X chromosome was analyzed by means of blot hybridization. The results indicated that the TBG gene is located in the midportion of the ...

  17. Langerhans Cell Histiocytosis in an Adult with Involvement of the Calvarium, Cerebral Cortex and Brainstem: Discussion of Pathophysiology and Rationale for the Use of Intravenous Immune Globulin

    Science.gov (United States)

    Dardis, Christopher; Aung, Thandar; Shapiro, William; Fortune, John; Coons, Stephen

    2015-01-01

    We report a case of Langerhans cell histiocytosis in a 64-year-old male who presented with symptoms and signs of brain involvement, including seizures and hypopituitarism. The diagnosis was confirmed with a biopsy of a lytic skull lesion. The disease affecting the bone showed no sign of progression following a short course of cladribine. Signs of temporal lobe involvement led to an additional biopsy, which showed signs of nonspecific neurodegeneration and which triggered status epilepticus. Lesions noted in the brainstem were typical for the paraneoplastic inflammation reported in this condition. These lesions improved after treatment with cladribine. They remained stable while on treatment with intravenous immune globulin. PMID:25873887

  18. Langerhans Cell Histiocytosis in an Adult with Involvement of the Calvarium, Cerebral Cortex and Brainstem: Discussion of Pathophysiology and Rationale for the Use of Intravenous Immune Globulin

    Directory of Open Access Journals (Sweden)

    Christopher Dardis

    2015-02-01

    Full Text Available We report a case of Langerhans cell histiocytosis in a 64-year-old male who presented with symptoms and signs of brain involvement, including seizures and hypopituitarism. The diagnosis was confirmed with a biopsy of a lytic skull lesion. The disease affecting the bone showed no sign of progression following a short course of cladribine. Signs of temporal lobe involvement led to an additional biopsy, which showed signs of nonspecific neurodegeneration and which triggered status epilepticus. Lesions noted in the brainstem were typical for the paraneoplastic inflammation reported in this condition. These lesions improved after treatment with cladribine. They remained stable while on treatment with intravenous immune globulin.

  19. High Fatality Rate of Epstein-Barr Virus-Associated Lymphoproliferative Disorder Occurring after Bone Marrow Transplantation with Rabbit Antithymocyte Globulin Conditioning Regimens

    OpenAIRE

    Peres, E; Savasan, S.; Klein, J; Abidi, M.; Dansey, R; Abella, E.

    2005-01-01

    Epstein-Barr virus (EBV)-associated lymphoproliferative disorder (EBV-LPD) following bone marrow transplantation can be fatal. The major risk factors for the development of EBV-LPD are ex vivo T-cell depletion or in vivo T-cell depletion with either antithymocyte globulin (ATG) or monoclonal anti-T-cell antibodies. Between March 1999 and January 2001, a total of 23 transplants with ATG of equine source (20 transplants) and ATG of rabbit source (3 transplants) used as part of the preparatory r...

  20. Effect of hepatitis B vaccine combined with hepatitis B immune globulin on infection in newborn infants of HBsAg-positive mothers

    Institute of Scientific and Technical Information of China (English)

    Si-Jia Yang; Jin-Hong Liu; Xue-Lian Tong; Ming Chen

    2016-01-01

    Objective:To investigate the effect of hepatitis B vaccine combined with hepatitis B immune globulin on infection in newborn infants of HBsAg-positive mothers. Methods:Two hundred newborn infants of HBsAg-positive mothers who had received prenatal examination and given birth to their child were selected as the study subjects and divided into the control group and the observation group in accordance with the voluntary principle. Newborn infants in the control group received 100 IU of vaccinations of hepatitis B vaccine and hepatitis B immune globulin at postnatal 24 h, 1 month and 6 months, while lying-in women in the observation group were continuously given 200 IU of immune globulin injection at the 27th, 30th, 33rd and 36th weeks of pregnancy. The infection condition and the influence of different delivery modes in infants of the two groups would be observed 12 months after birth. Results:The HBsAg positive rate and HBsAb positive rate of infants in the observation group were 1.00%and 59.00%respectively, while the control group’s were 10.00%and 73.00%. The HBsAg positive rate and HBsAb positive rate of infants in the observation group were significantly lower than those of the control group. The data showed statistical significances. Seven cases of infants born vaginally showed positive HBsAg. One of them belonged to the observation group and the other six were in the control group. Four cases of infants delivered by cesarean section showed positive HBsAg and they all belonged to the control group. The HBsAg positive rate of the observation group was obviously lower than that of the control group no matter what deliver mode those children had received. Conclusions:Hepatitis B vaccine combined with hepatitis B immune globulin could effectively decrease the morbidity rate of positive HBsAg for newborn infants no matter what birth way they had received.

  1. Fibroblast receptor for cell-substratum adhesion: studies on the interaction of baby hamster kidney cells with latex beads coated by cold insoluble globulin (plasma fibronectin)

    OpenAIRE

    1980-01-01

    Studies were carried out on the interactions of uncharged latex beads (0.76 micrometer) with baby hamster kidney cells. Binding of beads to the cells occurred if the beads were coated by cold insoluble globulin (CIG) (plasma fibronectin) but not if the beads were coated by bovine albumin. Bovine albumin-coated beads did not bind to the cells even in the presence of excess CIG in the incubation medium. Binding of beads occurred randomly over the entire surfaces of cells in suspension. However,...

  2. Safety and efficacy of indigenous equine antithymocyte globulin along with cyclosporine in subjects with acquired aplastic anemia.

    Science.gov (United States)

    Agarwal, M B; Jijina, Farah; Shah, Sandip; Malhotra, Pankaj; Damodar, Sharat; Ross, Cecil

    2015-06-01

    To confirm the safety and efficacy of an indigenous equine antithymocyte globulin (eATG) along with cyclosporine in Indian subjects with acquired aplastic anaemia. Subjects >2 years old with acquired aplastic anaemia were enrolled at six hospitals between April 2011 and February 2013, after approval from respective Ethics Committees. Equine ATG at a dose of 40 mg/kg/day was infused for 4 days. Efficacy analysis defined a priori, was in subjects, who had completed eATG treatment and followed-up on day 90 and/or 180. Complete response (CR) was defined as-transfusion independent, haemoglobin ≥11 g/dL, absolute neutrophil count (ANC) >1.5 10(9)/L and platelet ≥150 10(9)/L; partial response (PR) was transfusion independent, haemoglobin ≥8 g/dL, ANC >0.5 10(9)/L and platelet ≥20 10(9)/L; non responders were transfusion dependent. Lymphocyte subsets (CD 2, 3, 4 and 8) in the blood were tested on days 0 (pre eATG infusion), 3, 5, 7, 14 and 21 after eATG. Of the 30 subjects (two children pneumonia, one was withdrawn after the first dose of eATG due to jaundice and eight were lost to follow-up. The median age was 30 (9-58) years and weight was 57 (26-84) kg. On day 90, 12 of 30 subjects responded (CR 1, PR 11) and 15 of 30 (CR 2, PR 13) on day 180. The most common adverse event was fever related to eATG infusion. There were two serious adverse events (acute renal failure, febrile neutropenia) and both recovered with treatment. There were no unusual adverse events noted during the study period. Blood T lymphocytes showed a mean decrease of 91 % from baseline that recovered by day 21. We conclude that eATG is safe and in combination with cyclosporine showed overall response in 50 % of enrolled subjects. The trial was registered with the clinical trial registry-india (Registration no. CTRI/2012/03/002498). PMID:25825555

  3. Characterization of the lymphocyte membrane receptor for factor H (β1H- globulin) with an antibody to anti-factor H idiotype

    OpenAIRE

    Lambris, JD; Ross, GD

    1982-01-01

    Antibody to the binding site (idiotype) of anti-factor H was shown to have specificity for both B lymphocyte membrane H receptors and C3b. Goat F(ab’)(2) anti-human H was purified by absorption and elution from H agarose and used for rabbit immunization to produce anti-anti-H (aaH). After absorption with nonimmune goat IgG, (125)I-labeled aaH bound to B lymphocytes and to sheep erythrocytes coated with C3b (EC3b) but did not bind to T lymphocytes or to EC3d. All B cell- and C3b-specific activ...

  4. Pharmacokinetic and pharmacodynamic studies of two different rabbit antithymocyte globulin dosing regimens: results of a randomized trial.

    Science.gov (United States)

    Büchler, Matthias; Longuet, Hélène; Lemoine, Roxane; Herr, Florence; Gatault, Philippe; Thibault, Gilles; Ternant, David; Foulon, Christine; Pilorge, Bernadette; Lemay, Djamila; Sung, Crystal; Halimi, Jean-Michel; Baron, Christophe; Lebranchu, Yvon

    2013-03-01

    Rabbit antithymocyte globulin (rATG; Thymoglobulin(®)) is currently used to prevent acute rejection in kidney transplantation. The dose and regimen of rATG have not been optimized. Moreover, the impact of different treatment regimens on T-cell phenotype reconstitution remains unknown. We conducted a prospective randomized study of 17 renal transplant patients to determine the pharmacokinetics of total and active (bound to human cells) rATG and T-cell phenotype reconstitution after rATG administration. Patients received rATG at a total dose of 6mg/kg, administered either as 1.5mg/kg/day on days 0-3 (Group 1, n=8) or 3mg/kg on days 0 and 3 (Group 2, n=9). All patients received tacrolimus, mycophenolate mofetil and steroids. Blood samples were assayed for total rATG by enzyme linked immunosorbent assay and active rATG by flow cytometry. Maximum concentrations and terminal half-lives were similar between the two groups but at month 3 Group 1 had significantly lower values for total rATG (concentration was 6.2±1.1μg/mL versus 10.2±2.9μg/mL in Group 2, p=0.027) and total rATG dose-normalized AUC (374±83dayg/mL versus 508±149dayg/mL in Group 2, p=0.046). Time to sub-therapeutic levels (<1μg/mL) of active rATG was significantly shorter in Group 1 (18.75±6.9days versus 20±7.5days in Group 2, p<0.001). rATG induced significant depletion followed by slow reconstitution of CD3(+), CD4(+) and CD8(+) cells, with no marked differences between groups. B-cell count was unaffected, whereas CD3(-)CD56(+) NK-cell depletion was observed in both groups. rATG induced a significant decrease in the proportion of naïve CD4(+) T-cells, which plateaued after month 1 in Group 1 and after month 6 in Group 2. The proportion of central memory CD4(+) T-cells increased to a similar extent in both groups (Group 1: 38±18% at baseline, 74±23% at one year, p=0.009; Group 2: 32±14% at baseline, 65±14% at one year, p=0.001). In conclusion, our results suggest that the dosing regimen for r

  5. Association of the pituitary-testicular axis function and sex hormone-binding globulin with melatonin secretion in morbidly obese men

    International Nuclear Information System (INIS)

    A possible relationship between melatonin (MEL) secretion and pituitary-testicular function as well as the circadian rhythmicity of serum MEL, lutropin (LH), folitropin (FSH), estradiol (E2), total testosterone (T) and sex hormone-binding globulin (SHBG) were evaluated in 16 men with the primary obesity (body mass index - BMI > 43 kg/m2; waist-to-hip circumference ratio - WHR > 1.0) and in 17 healthy volunteers with normal body weight. The mean 24-h MEL level was significantly higher in obese patients than in healthy control individuals. Moreover, all obese men showed some abnormalities of MEL circadian pattern such as decreased ratio between day and night MEL levels, abnormal secretory peaks during the light hours and lower interindividual variability for timing amplitude. Abnormal circadian variations of MEL were associated with reduced 24-h mean values of LH, FSH, T and SHBG, whereas E2 levels were elevated. (author). 49 refs, 4 figs, 2 tabs

  6. The effect of triiodothyronine (L-T3) and weight loss on sex-hormone binding globulin (SHBG) in hirsute obese women

    International Nuclear Information System (INIS)

    The effect of a 60-day period of treatment with L-T3 (200 μg/day) was evaluated in hirsute obese women and normal controls. The obese patients were also submitted to a low calorie high protein. Mean initial weight significantly declined with a significant lowering of the mean DHEA-S leves. There was changes in serum T3 rose in both obese and normal patients. Serum sex-hormone binding globulin levels at baseline studies were significantly higher in normal controls than in obese patients. At the end of de 60-day period of L-T3 administration it was observed in both groups a 2-4 fold increase in serum SHBG levels but normal controls had significantly higher mean values than obese individuals. (M.A.C.)

  7. "Bound" complement in the skin of patients with chronic discoid lupus erythematosus and systemic lupus erythematosus

    NARCIS (Netherlands)

    Kalsbeek, G.L.; Cormane, R.H.

    1964-01-01

    At about the same time and independently of each other, Burnham, Neblett, and Fine (1963), and Cormane (1963) were able to show with the direct immunofluorescent method, using commercially prepared fluoresceinlabelled goat anti-human globulin and fluoresceinlabelled goat anti-human gamma-globulin, a

  8. A simple and rapid Hepatitis A Virus (HAV titration assay based on antibiotic resistance of infected cells: evaluation of the HAV neutralization potency of human immune globulin preparations

    Directory of Open Access Journals (Sweden)

    Kaplan Gerardo G

    2008-12-01

    Full Text Available Abstract Background Hepatitis A virus (HAV, the causative agent of acute hepatitis in humans, is an atypical Picornaviridae that grows poorly in cell culture. HAV titrations are laborious and time-consuming because the virus in general does not cause cytopathic effect and is detected by immunochemical or molecular probes. Simple HAV titration assays could be developed using currently available viral construct containing selectable markers. Results We developed an antibiotic resistance titration assay (ARTA based on the infection of human hepatoma cells with a wild type HAV construct containing a blasticidin (Bsd resistance gene. Human hepatoma cells infected with the HAV-Bsd construct survived selection with 2 μg/ml of blasticidin whereas uninfected cells died within a few days. At 8 days postinfection, the color of the pH indicator phenol red in cell culture media correlated with the presence of HAV-Bsd-infected blasticidin-resistant cells: an orange-to-yellow color indicated the presence of growing cells whereas a pink-to-purple color indicated that the cells were dead. HAV-Bsd titers were determined by an endpoint dilution assay based on the color of the cell culture medium scoring orange-to-yellow wells as positive and pink-to-purple wells as negative for HAV. As a proof-of-concept, we used the ARTA to evaluate the HAV neutralization potency of two commercially available human immune globulin (IG preparations and a WHO International Standard for anti-HAV. The three IG preparations contained comparable levels of anti-HAV antibodies that neutralized approximately 1.5 log of HAV-Bsd. Similar neutralization results were obtained in the absence of blasticidin by an endpoint dilution ELISA at 2 weeks postinfection. Conclusion The ARTA is a simple and rapid method to determine HAV titers without using HAV-specific probes. We determined the HAV neutralization potency of human IG preparations in 8 days by ARTA compared to the 14 days required by the

  9. Action of antithymocyte globulin on normal human erythroid progenitor cell proliferation in vitro: erythropoietic growth-enhancing factors are released from marrow accessory cells.

    Science.gov (United States)

    Mangan, K F; D'Alessandro, L; Mullaney, M T

    1986-04-01

    Studies were undertaken to determine the mechanism of action of a horse antithymocyte globulin preparation (ATGAM) (HATG) and its control preparation of horse gamma globulin (HIgG) on the proliferation of normal human marrow and blood erythroid progenitor cells (CFU-E, BFU-E) in vitro. In preincubation studies with marrow mononuclear cells and complement, HATG did not significantly augment CFU-E or BFU-E growth greater than that expected because of removal of marrow T cells by this agent. However, direct addition of HATG but not HIgG to marrow cultures significantly stimulated CFU-E and BFU-E up to two to four times that of media or HIgG controls (P less than 0.05). The peak effect was observed at 10 to 100 micrograms/ml HATG; HATG was toxic at 1000 micrograms/ml. By contrast, the OKT3 monoclonal antibody was less stimulatory than HATG. The in vitro erythropoietic stimulatory effect of HATG was dependent on the presence of accessory cells because removal of T cells or monocytes (less than 2% to 5%) or adsorptions of HATG with T cells or monocytes reduced its stimulatory effect, highly purified BFU-E nearly devoid of accessory cells required irradiated accessory cells for demonstration of the HATG stimulatory effect, and an erythroid burst-promoting activity was released from T cells or unseparated mononuclear cells in the presence of HATG but not HIgG. The HATG enhancing effect was optimal in the first 96 hours of cultures in the presence of erythropoietin, and was reproducible with three separate lots of HATG. Up to 16% of HATG-stimulated erythroid colonies expressed nonerythroid lineage cells. Iron 59 incorporation into heme of CFU-E- or BFU-E-derived colonies was augmented equally by HATG or HIgG at 10 micrograms/ml. Erythropoietin dose-response curves and studies with antierythropoietin sera suggested that HATG also increased the sensitivity of erythroid progenitor cells to very low concentrations of erythropoietin. We conclude that HATG but not HIgG control

  10. Combination therapy with antibiotics and anthrax immune globulin intravenous (AIGIV is potentially more effective than antibiotics alone in rabbit model of inhalational anthrax.

    Directory of Open Access Journals (Sweden)

    Srinivas Kammanadiminti

    Full Text Available BACKGROUND: We have evaluated the therapeutic efficacy of AIGIV when given in combination with levofloxacin and the effective window of treatment to assess the added benefit provided by AIGIV over standard antibiotic treatment alone in a New Zealand white rabbit model of inhalational anthrax. METHODS: Rabbits were exposed to lethal dose of aerosolized spores of Bacillus anthracis (Ames strain and treated intravenously with either placebo, (normal immune globulin intravenous, IGIV or 15 U/kg of AIGIV, along with oral levofloxacin treatment at various time points (30-96 hours after anthrax exposure. RESULTS: The majority of treated animals (>88% survived in both treatment groups when treatment was initiated within 60 hours of post-exposure. However, reduced survival of 55%, 33% and 25% was observed for placebo + levofloxacin group when the treatment was initiated at 72, 84 and 96 hours post-exposure, respectively. Conversely, a survival rate of 65%, 40% and 71% was observed in the AIGIV + levofloxacin treated groups at these time points. CONCLUSIONS: The combination of AIGIV with antibiotics provided an improvement in survival compared to levofloxacin treatment alone when treatment was delayed up to 96 hours post-anthrax exposure. Additionally, AIGIV treatment when given as an adjunct therapy at any of the time points tested did not interfere with the efficacy of levofloxacin.

  11. Influx of testosterone-binding globulin (TeBG) and TeBG-bound sex steroid hormones into rat testis and prostate

    Energy Technology Data Exchange (ETDEWEB)

    Sakiyama, R.; Pardridge, W.M.; Musto, N.A.

    1988-07-01

    The availability of testosterone and estradiol to Sertoli and prostate cells is dependent upon 1) the permeability properties of the blood-tubular barrier (BTB) of the testis or prostate cell membrane, and 2) sex steroid binding to plasma proteins, such as albumin or testosterone-binding globulin (TeBG). Sex steroid influx into these tissues was studied after in vivo arterial bolus injections of (/sup 3/H)testosterone or (/sup 3/H)estradiol in anesthetized rats. Both testosterone and estradiol were readily cleared across the BTB or prostate cell membrane in the absence of plasma proteins and in the presence of human pregnancy serum, in which testosterone or estradiol are 80-95% distributed to TeBG. The extravascular extraction of (/sup 3/H)TeBG across the BTB or prostate plasma membrane (73 +/- 2% (+/- SE) and 92 +/- 9%, respectively) was significantly greater than extraction of (/sup 3/H)albumin or other plasma space markers and indicative of a rapid first pass clearance of TeBG by Sertoli or prostate cells. In summary, these studies indicate that 1) testosterone and estradiol are readily cleared by Sertoli and prostate cells; 2) albumin- and TeBG-bound sex steroids represent the major circulating pool of bioavailable hormone for testis or prostate; and 3) the TeBG-sex steroid complex may be nearly completely available for influx through the BTB or prostate plasma membrane.

  12. Opportunistic virus DNA levels after pediatric stem cell transplantation: serostatus matching, anti-thymocyte globulin, and total body irradiation are additive risk factors.

    Science.gov (United States)

    Kullberg-Lindh, C; Mellgren, K; Friman, V; Fasth, A; Ascher, H; Nilsson, S; Lindh, M

    2011-04-01

    Viral opportunistic infections remain a threat to survival after stem cell transplantation (SCT). We retrospectively investigated infections caused by cytomegalovirus (CMV), Epstein-Barr virus (EBV), human herpesvirus type 6 (HHV6), or adenovirus (AdV) during the first 6-12 months after pediatric SCT. Serum samples from 47 consecutive patients were analyzed by quantitative real-time polymerase chain reaction assay. DNAemia at any time point occurred for CMV in 47%, for EBV in 45%, for HHV6 in 28%, and for AdV in 28%. Three patients (6.3%) died of CMV-, EBV-, or AdV-related complications 4, 9, and 24 weeks after SCT, respectively, representing 21% of total mortality. These 3 cases were clearly distinguishable by DNAemia increasing to high levels. Serum positivity for CMV immunoglobulin G in either recipient or donor at the time of SCT, total body irradiation, and anti-thymocyte globulin conditioning were independent risk factors for high CMV or EBV DNA levels. We conclude that DNAemia levels help to distinguish significant viral infections, and that surveillance and prophylactic measures should be focused on patients with risk factors in whom viral complications rapidly can become fatal.

  13. Effect of atrazine and fenitrothion at no-observed-effect-levels (NOEL) on amphibian and mammalian corticosterone-binding-globulin (CBG).

    Science.gov (United States)

    Hernández, Sandra E; Sernia, Conrad; Bradley, Adrian J

    2014-11-01

    This study determines the effect of atrazine and fenitrothion no-observed-effect-levels (NOEL) on the binding of corticosterone (B) to corticosterone-binding-globulin (CBG) in an amphibian and a mammal. Plasma from five cane toads and five Wistar rats was exposed to atrazine and fenitrothion at the NOEL approved for Australian fresh water residues and by the World Health Organization (WHO). The concentration required to displace 50% (IC50) of B binding to CBG was determined by a competitive microdialysis protein assay. Competition studies showed that both atrazine and fenitrothion at NOEL are able to compete with B for CBG binding sites in toad and rat plasma. The IC50 levels for atrazine in toads and rats were 0.004 nmol/l and 0.09 nmol/l respectively. In the case of fenitrothion the IC50 level found in toads was 0.007 nmol/l, and 0.025 nmol/l in rats. Plasma dilution curves showed parallelism with the curve of B, demonstrating that these agro-chemicals are competitively inhibiting binding to CBG. The displacement of B by atrazine and fenitrothion would affect the total:free ratio of B and consequently disrupt the normal stress response. This is the first time that the potential disruptive effect of atrazine and fenitrothion on B-CBG interaction at the NOELs has been demonstrated in amphibian and mammalian models.

  14. Incidence and Pattern of Graft-versus-Host Disease in Patients Undergoing Allogeneic Transplantation after Nonmyeloablative Conditioning with Total Lymphoid Irradiation and Antithymocyte Globulin

    Directory of Open Access Journals (Sweden)

    Lauren Veltri

    2013-01-01

    Full Text Available Nonmyeloablative (NMA conditioning with total lymphoid irradiation and antithymocyte globulin (TLI/ATG has been shown to protect against acute graft-versus-host disease (GVHD. We report here our institutional experience with allogeneic transplantation following NMA conditioning with TLI/ATG (. GVHD prophylaxis consisted of a combination of a calcineurin inhibitor and mycophenolate mofetil. Median patient age was 59 years. The median followup of surviving patients is 545 days. One patient experienced primary graft rejection. The median time to neutrophil engraftment was 18 days and platelet engraftment was 9.5 days. The cumulative incidence (CI of grade II–IV acute GVHD at day +100 was 28.6% and 38.1% at day +180. The CI for grade III-IV acute GVHD was 28.6% at day +180. CI of chronic GVHD was 45.2% at 1 year. The CI of disease relapse was 9.5% at 1 year. The rate of nonrelapse mortality (NRM was 0% at day +100 and only 9.5% at 1 year. The overall and progression free survival at 1 year was 81% and 80.4%, respectively. Our limited, retrospective data show encouraging relapse and NRM rates with TLI/ATG-based NMA conditioning, but with higher than previously reported rates of acute and chronic GVHD, underscoring the need for novel strategies designed to effectively prevent GVHD.

  15. Complex histopathologic response in rat kidney to oral β-myrcene: an unusual dose-related nephrosis and low-dose alpha2u-globulin nephropathy.

    Science.gov (United States)

    Cesta, Mark F; Hard, Gordon C; Boyce, John T; Ryan, Michael J; Chan, Po C; Sills, Robert C

    2013-01-01

    Oral gavage studies with β-myrcene in male F344 rats showed a complex renal pathology comprising both alpha2u-globulin (α2u-g) nephropathy, an unusual nephrosis involving the outer stripe of outer medulla (OSOM), and an increased incidence of renal tubule tumors by 2 years. In the 90-day and 2-year studies, respectively, α2u-g nephropathy and linear papillary mineralization were observed in males at the two lower doses but were absent from the high dose. Nephrosis was characterized by dilation of the S3 tubules, nuclear enlargement (including karyomegaly), and luminal pyknotic cells, all in the outermost OSOM. Nephrosis was minimal at the higher doses in the 90-day study, but progressed to a severe grade in males dosed with 1,000 mg/kg for 2 years. Renal tubule tumors developed in treated groups with incidences up to 30% in the 250 and 500 mg/kg male dose groups. Tumors at the lower doses in males may have been associated with α2u-g nephropathy, while those at higher doses in both sexes may have been due to the nephrosis. Because β-myrcene induced a complex spectrum of renal pathology, the α2u-g nephropathy mechanism cannot be the sole mechanism of carcinogenesis in these rats.

  16. Ovarian cancer risk and common variation in the sex hormone-binding globulin gene: a population-based case-control study

    International Nuclear Information System (INIS)

    The sex hormone-binding globulin (SHBG) is a carrier protein that modulates the bio-availability of serum sex steroid hormones, which may be involved in ovarian cancer. We evaluated whether common genetic variation in SHBG and its 3' neighbor ATP1B2, in linkage disequilibrium, is associated with the risk of epithelial ovarian cancer. The study population included 264 women with ovarian carcinoma and 625 controls participating in a population-based case-control study in Poland. Five common single nucleotide polymorphisms (SNPs) in SHGB and five in ATP1B2 were selected to capture most common variation in this region. None of the SNPs evaluated was significantly associated with ovarian cancer risk, including the putative functional SNPs SHBG D356N (rs6259) and -67G>A 5'UTR (rs1799941). However, our data were consistent with a decreased ovarian cancer risk associated with the variant alleles for these two SNPs, which have been previously associated with increased circulating levels of SHBG. These data do not support a substantial association between common genetic variation in SHBG and ovarian cancer risk

  17. Anti-thymocyte globulin could improve the outcome of allogeneic hematopoietic stem cell transplantation in patients with highly aggressive T-cell tumors

    International Nuclear Information System (INIS)

    The early experiment result in our hospital showed that anti-thymocyte globulin (ATG) inhibited the proliferation of lymphoid tumor cells in the T-cell tumors. We used the ATG as the part of the conditioning regimen and to evaluate the long-term anti-leukemia effect, the safety and complication in the patients with highly aggressive T-cell lymphomas. Twenty-three patients were enrolled into this study. At the time of transplant, six patients reached first or subsequent complete response, three patients had a partial remission and 14 patients had relapsed or primary refractory disease. The conditioning regimen consisted of ATG, total body irradiation, toposide and cyclophosphamide. The complete remission rate after transplant was 95.7%. At a median follow-up time of 25 months, 16 (69.6%) patients are alive and free from diseases, including nine patients in refractory and progressive disease. Seven patients died after transplant, five from relapse and two from treatment-related complications. The incidence of grades II–IV acute graft-vs-host disease (GvHD) was 39.1%. The maximum cumulative incidence of chronic GvHD was 30%. The most frequent and severe conditioning-related toxicities observed in 8 out of 23 patients were grades III/IV infections during cytopenia. Thus, ATG-based conditioning is a feasible and effective alternative for patients with highly aggressive T-cell tumors

  18. Ovarian cancer risk and common variation in the sex hormone-binding globulin gene: a population-based case-control study

    Directory of Open Access Journals (Sweden)

    Yeager Meredith

    2007-04-01

    Full Text Available Abstract Background The sex hormone-binding globulin (SHBG is a carrier protein that modulates the bio-availability of serum sex steroid hormones, which may be involved in ovarian cancer. We evaluated whether common genetic variation in SHBG and its 3' neighbor ATP1B2, in linkage disequilibrium, is associated with the risk of epithelial ovarian cancer. Methods The study population included 264 women with ovarian carcinoma and 625 controls participating in a population-based case-control study in Poland. Five common single nucleotide polymorphisms (SNPs in SHGB and five in ATP1B2 were selected to capture most common variation in this region. Results None of the SNPs evaluated was significantly associated with ovarian cancer risk, including the putative functional SNPs SHBG D356N (rs6259 and -67G>A 5'UTR (rs1799941. However, our data were consistent with a decreased ovarian cancer risk associated with the variant alleles for these two SNPs, which have been previously associated with increased circulating levels of SHBG. Conclusion These data do not support a substantial association between common genetic variation in SHBG and ovarian cancer risk.

  19. Primary structure of human corticosteroid binding globulin, deduced from hepatic and pulmonary cDNAs, exhibits homology with serine protease inhibitors

    International Nuclear Information System (INIS)

    The authors have isolated and sequenced cDNAs for corticosteroid binding globulin (CBG) prepared from human liver and lung mRNAs. The results indicate that CBG mRNA is relatively abundant in the liver but is also present in the lung, testis, and kidney. The liver CBG cDNA contains an open reading frame for a 405-amino acid (M/sub r/ 45,149) polypeptide. This includes a predominantly hydrophobic, leader sequence of 22 residues that precedes the known NH2-terminal sequence of human CBG. We, therefore, predict that the mature protein is composed of 383 amino acids and is a polypeptide of M/sub r/ 42,646. A second, in-frame, 72-base-pair cistron of unknown significance exists between the TAA termination codon for CBG and a possible polyadenylylation signal (AATAAA) located 16 nucleotides before the polyadenylylation site. The deduced amino acid sequence of mature CBG contains two cysteine residues and consensus sequences for the attachment of six possible N-linked oligosaccharide chains. The sequences of the human lung and liver CBG cDNAs differ by only one nucleotide within the proposed leader sequence,and they attribute this to a point mutation. No sequence homology was found between CBG and other steroid binding proteins, but there is a remarkable similarity between the amino acid sequences of CBG and of α1-antitrypsin, and this extends to other members of the serpin (serine protease inhibitor) superfamily

  20. Boron labeled rabbit anti-rat fibrin and goat anti-rabbit gamma globulin antibodies and their potential for slow neutron capture therapy of tumors

    International Nuclear Information System (INIS)

    The therapeutic effectiveness of slow neutron capture therapy is currently dependent upon achieving a high concentration gradient of boron between tumor and normal tissue. Labeling of anti-tumor or anti-tumor site antibodies with boron containing compounds could provide this high therapeutic value. Anti-rat fibrin antibodies, which show considerable localization at the site of several transplantable rat tumors, were labeled with 4-boronophenylalanine (4-BPA) using the N-carboxy anhydride procedure. Activity of these labeled antibodies was studied by modifying the test for fibrinogen concentration of hemophiliac blood. The number of 4-BPAs bound to each active antibody was determined indirectly using the fluorimetric test for phenylalanine concentration in serum. These tests showed that labeled antibodies retained their activity, and it was possible to add up to fifty 4-BPAs per active antibody. The indirect approach to achieving a high therapeutic value of boron was also investigated. This procedure involves labeling anti-immunoglobulin antibodies that bind to anti-tumor antibodies which are already bound to their respective antigens. Indirect labeling has the potential of increasing the therapeutic value by a factor of ten over the direct approach. Activity of labeled goat anti-rabbit gamma globulin (RGG) antibodies was studied by radial immunodiffusion and passive hemagglutination. The number of 4-BPAs bound to each active antibody was determined indirectly by fluorimetry. These labeled antibodies also retained their activity, and it was possible to add upwards of forty 4-BPAs per active antibody

  1. 纯化和标记抗人轻链β2m单克隆抗体的方法%Efficient purification and label of anti-human beta 2-microglobulin light chain monoclonal antibody

    Institute of Scientific and Technical Information of China (English)

    阮光萍; 姚翔; 庞荣清; 汪兴明; 戴莹; 潘兴华

    2011-01-01

    BACKGROUND: The inoculation of hybridoma cell strain onto mouse abdominal cavity may obtain ascites containing mass antibody. Previous method to purify monoclonal antibody in ascites is complex and difficult to operate.OBJECTIVE: To prepare, purify and label anti-human leukocyte antigen (HLA)-I class molecule light chain monoclonal antibody, and to detect the expression of tumor cell surface HLA-I class molecules. METHODS: Hybridoma cells were inoculated onto the mouse abdominal cavity. Ascites containing anti-human light chain beta2-microglobulin antibody were obtained and purified with the modified caprylic acid-ammonium sulfate method. The purified monoclonal antibody was labeled with fluorescein isothiocyanate to detect peripheral blood mononuclear cells, T2 cells expressing blank HLA-A2 molecule and K562 cells surface HLA-I class molecules. The expression of HLA-I class molecules was determined by using flow cytometry and fluorescent microscopy. RESULTS AND CONCLUSION: The purified anti-human light chain beta2-microglobulin-fluorescein isothiocyanate monoclonal antibodies accounted for 96% purity. Flow cytometry results showed that, the HLA-I class molecules were highly expressed in peripheral blood mononuclear cells surface, lowly expressed in T2 cells, and not expressed in K562 cells surface. It is a simple and convenient method to purify ascites with the modified caprylic acid-ammonium sulfate method, and according prepare anti-human light chain beta2-microglobulin-fluorescein isothiocyanate. This method is effective to distinguish the levels of HLA-I class molecules expressed in various cells.%背景:通过杂交瘤细胞株接种小鼠腹腔可获得含大量抗体的腹水,但以往纯化腹水中单克隆抗的方法较复杂,不易操作.目的:制备、纯化和标记抗人类白细胞抗原Ⅰ类分子轻链的单克隆抗体,以检测肿瘤细胞表面的人类白细胞抗原Ⅰ类分子的表达.方法:将杂交瘤细胞接种至小

  2. Transgenic rabbits that overexpress the neonatal Fc receptor (FcRn generate higher quantities and improved qualities of anti-thymocyte globulin (ATG.

    Directory of Open Access Journals (Sweden)

    Mária Baranyi

    Full Text Available Immune suppression with rabbit anti-thymocyte globulin (rATG is a well-established therapeutic concept for preventing host rejection of transplanted organs and graft versus host disease. Increasing the efficiency of rATG production by reducing the number of animals would be highly beneficial to lower cost and to improve quality standards. We have developed transgenic (Tg mice and rabbits that overexpress the neonatal Fc receptor (FcRn and have shown an augmented humoral immune response in these animals. To test whether our FcRn Tg rabbits produced rATG more efficiently, we immunized them and their New Zealand White controls with live Jurkat cells. By day 21 after immunization, Tg animals produced significantly, 1.5 times higher amount of total IgG compared to their wt littermates. Also, the binding efficiency of Tg sera to Jurkat cells and their complement-mediated cytotoxicity was significantly higher. The purified Tg IgG preparation contained 2.6 the amount of Jurkat specific IgG as the wt preparation analyzed by complement-mediated lysis, suggesting greater antigen-specific B cell activation in the Tg rabbits. To test this hypothesis, immunization with ovalbumin and human α1-antitrypsin was performed, resulting in significantly greater numbers of antigen-specific B-cells in the FcRn Tg rabbits as compared with wt controls. The shift towards significantly larger populations of antigen-specific B cells relative to the non-specific B cell pool is further corroborated by our previous findings in FcRn Tg mice. Consequently, our FcRn Tg rabbits have the potential to offer substantial qualitative and quantitative improvements for the production of rATG and other polyclonal or monoclonal antibodies.

  3. Efficacy, safety, and dose response of intravenous anti-D immune globulin (WinRho SDF) for the treatment of idiopathic thrombocytopenic purpura in children.

    Science.gov (United States)

    Freiberg, A; Mauger, D

    1998-01-01

    We analyzed data from 20 children treated for acute or chronic idiopathic (immune) thrombocytopenic purpura (ITP) at a single institution to determine the relationship between dose of intravenous anti-D immune globulin (WinRho SDF; Nabi, Boca Raton, FL), increase in platelet count, and decrease in hemoglobin in the therapy of ITP. Higher doses of anti-D were clearly associated with a greater therapeutic response in the platelet count, with no increase in hemolysis for both acute and chronic ITP. A significant correlation was found between dose and peak increase in platelet count measured in the 14 days following administration. This effect was present for both acute ITP (17 infusions, P = .0001) and chronic ITP (30 infusions, P = .038). Although hemolysis was seen in nearly all infusions, with a median hemoglobin fall of 1.9 g/dL (range, 0 to 4.2), the decrease in hemoglobin was greater than 2.5 for only three infusions, and the largest fall in hemoglobin (4.2) was in a child with an underlying hemolytic anemia. Furthermore, for both acute and chronic ITP there was no relationship between the decrease in hemoglobin and the dose given (P = .22), nor between the increase in platelet count and fall in hemoglobin (P = .27). This analysis supports the use of higher doses of anti-D for the treatment of ITP, and demonstrates the need for a trial of high-dose anti-D (>100 microg/kg) in acute and chronic ITP. PMID:9523746

  4. Comparison between spousal donor transplantation treated with anti-thymocyte globulin induction therapy and, living related donor transplantation treated with standard immunosuppression

    Directory of Open Access Journals (Sweden)

    Erkan Demir

    2014-01-01

    Full Text Available The worldwide shortage of organs available for transplantation has led to the use of living-unrelated kidney donors. In this context, spouses represent an important source of organ donors. We compared the allograft outcomes of spousal donor transplantation (SDT with anti-thymocyte globulin (ATG induction therapy and living related donor transplantation (LRDT with triple immonosuppression and basiliximab, in addition. Among the 335 living and deceased donor kidney transplantations performed between April 2001 and June 2010, there were 274 living donor kidney transplantations including 34 SDT and 240 LRDT. The minimum follow-up period was 36 months. All recipients of SDT received ATG (1.5 mg/kg induction therapy, which was stopped five to seven days after surgery. Maintenance immunosuppression included tacrolimus (TAC, mycophenolate mofetil (MMF and prednisolone. LRDT recipients received triple immunosuppressive protocol consisting of cyclosporine or TAC, MMF and prednisolone, in addition to basiliximab. There was a significant difference between the two groups in recipient age, while pre-operative duration on dialysis, recipient sex and donor age and sex were not significantly different. There was also a significant difference between the two groups in the number of human leukocyte antigen (HLA mismatches. The 1-, 3- and 5-year graft survival rates of SDT were 94.1%, 88.2% and 79.4%, respectively, and the frequency of acute rejection episodes was 5.8% (two cases. The 1-, 3- and 5-year graft survival rates of LRDT were 95.8%, 91.6% and 83.3%, respectively, with the frequency of acute rejection being 16.2%. The graft survival rates of SDT were as good as LRDT, while the acute rejection rates in SDT were lower than in LRDT, although the difference was not statistically different (P = 0.13.

  5. Comparison between spousal donor transplantation treated with anti-thymocyte globulin induction therapy and, living related donor transplantation treated with standard immunosuppression.

    Science.gov (United States)

    Demir, Erkan; Paydas, Saime; Erken, Ugur

    2014-05-01

    The worldwide shortage of organs available for transplantation has led to the use of living-unrelated kidney donors. In this context, spouses represent an important source of organ donors. We compared the allograft outcomes of spousal donor transplantation (SDT) with anti-thymocyte globulin (ATG) induction therapy and living related donor transplantation (LRDT) with triple immonosuppression and basiliximab, in addition. Among the 335 living and deceased donor kidney transplantations performed between April 2001 and June 2010, there were 274 living donor kidney transplantations including 34 SDT and 240 LRDT. The minimum follow-up period was 36 months. All recipients of SDT received ATG (1.5 mg/kg) induction therapy, which was stopped five to seven days after surgery. Maintenance immunosuppression included tacrolimus (TAC), mycophenolate mofetil (MMF) and prednisolone. LRDT recipients received triple immunosuppressive protocol consisting of cyclosporine or TAC, MMF and prednisolone, in addition to basiliximab. There was a significant difference between the two groups in recipient age, while pre-operative duration on dialysis, recipient sex and donor age and sex were not significantly different. There was also a significant difference between the two groups in the number of human leukocyte antigen (HLA) mismatches. The 1-, 3- and 5-year graft survival rates of SDT were 94.1%, 88.2% and 79.4%, respectively, and the frequency of acute rejection episodes was 5.8% (two cases). The 1-, 3- and 5-year graft survival rates of LRDT were 95.8%, 91.6% and 83.3%, respectively, with the frequency of acute rejection being 16.2%. The graft survival rates of SDT were as good as LRDT, while the acute rejection rates in SDT were lower than in LRDT, although the difference was not statistically different (P = 0.13).

  6. Associations of sex hormone-binding globulin and testosterone with diabetes among men and women (the Saku Diabetes study: a case control study

    Directory of Open Access Journals (Sweden)

    Goto Atsushi

    2012-10-01

    Full Text Available Abstract Background Sex hormone-binding globulin (SHBG levels and sex hormones have been implicated in the pathogenesis of type 2 diabetes and cardiovascular diseases. As fatty liver has been suggested to be a major determinant of SHBG levels, we examined whether the associations of SHBG and testosterone with diabetes were independent of fatty liver. Methods We conducted a case–control study that included 300 diabetes cases (215 men and 85 women and 300 matched controls from the Saku cohort study. Diabetes was defined by either fasting plasma glucose levels ≥126 mg/dL, 2-h post-load glucose levels ≥200 mg/dL after a 75 g oral glucose tolerance test, or diabetes diagnosed by physicians. We fitted conditional logistic regression models to examine the associations between SHBG and total testosterone levels with diabetes by sex. To evaluate the impact of fatty liver, we used the fatty liver index (FLI, a validated measure derived from serum triglyceride levels, body mass index (BMI, waist circumference, and γ-glutamyltransferase levels. Results After adjusting for age, family history of diabetes, smoking, physical activity, BMI, and FLI, SHBG levels were inversely associated with diabetes among women (odds ratio [OR] comparing the highest with the lowest quartiles, 0.13 [95% confidence interval {CI}, 0.02–0.96], but not among men. Similar patterns were observed in a subgroup analysis restricted to postmenopausal women"(OR, 0.12 [95% CI, 0.01–1.17]. In contrast, testosterone levels were inversely associated with diabetes among men (OR, 0.45 [95% CI, 0.23–0.89], but not among women. Conclusions Our findings suggest that SHBG in women and testosterone in men may be inversely associated with diabetes.

  7. Combined N-glycome and N-glycoproteome analysis of the Lotus japonicus seed globulin fraction shows conservation of protein structure and glycosylation in legumes.

    Science.gov (United States)

    Dam, Svend; Thaysen-Andersen, Morten; Stenkjær, Eva; Lorentzen, Andrea; Roepstorff, Peter; Packer, Nicolle H; Stougaard, Jens

    2013-07-01

    Legume food allergy, such as allergy toward peanuts and soybeans, is a health issue predicted to worsen as dietary advice recommends higher intake of legume-based foods. Lotus japonicus (Lotus) is an established legume plant model system for studies of symbiotic and pathogenic microbial interactions and, due to its well characterized genotype/phenotype and easily manipulated genome, may also be suitable for studies of legume food allergy. Here we present a comprehensive study of the Lotus N-glycoproteome. The global and site-specific N-glycan structures of Lotus seed globulins were analyzed using mass spectrometry-based glycomics and glycoproteomics techniques. In total, 19 N-glycan structures comprising high mannose (∼20%), pauci-mannosidic (∼40%), and complex forms (∼40%) were determined. The pauci-mannosidic and complex N-glycans contained high amounts of the typical plant determinants β-1,2-xylose and α-1,3-fucose. Two abundant Lotus seed N-glycoproteins were site-specifically profiled; a predicted lectin containing two fully occupied N-glycosylation sites carried predominantly pauci-mannosidic structures in different distributions. In contrast, Lotus convicilin storage protein 2 (LCP2) carried exclusively high mannose N-glycans similar to its homologue, Ara h 1, which is the major allergen in peanut. In silico investigation confirmed that peanut Ara h 1 and Lotus LCP2 are highly similar at the primary and higher protein structure levels. Hence, we suggest that Lotus has the potential to serve as a model system for studying the role of seed proteins and their glycosylation in food allergy.

  8. Low levels of sex hormone-binding globulin and hyperproinsulinemia as markers of increased pancreatic ß-cell demand in men

    Directory of Open Access Journals (Sweden)

    A.F. Reis

    1998-12-01

    Full Text Available Low levels of sex hormone-binding globulin (SHBG are considered to be an indirect index of hyperinsulinemia, predicting the later onset of diabetes mellitus type 2. In the insulin resistance state and in the presence of an increased pancreatic ß-cell demand (e.g. obesity both absolute and relative increases in proinsulin secretion occur. In the present study we investigated the correlation between SHBG and pancreatic ß-cell secretion in men with different body compositions. Eighteen young men (30.0 ± 2.4 years with normal glucose tolerance and body mass indexes (BMI ranging from 22.6 to 43.2 kg/m2 were submitted to an oral glucose tolerance test (75 g and baseline and 120-min blood samples were used to determine insulin, proinsulin and C-peptide by specific immunoassays. Baseline SHBG values were significantly correlated with baseline insulin (r = -0.58, P28 kg/m2, N = 8 and nonobese (BMI £25 kg/m2, N = 10 groups, significantly lower levels of SHBG were found in the obese subjects. The obese group had significantly higher baseline proinsulin, C-peptide and 120-min proinsulin and insulin levels. For the first time using a specific assay for insulin determination, a strong inverse correlation between insulinemia and SHBG levels was confirmed. The finding of a strong negative correlation between SHBG levels and pancreatic ß-cell secretion, mainly for the 120-min post-glucose load proinsulin levels, reinforces the concept that low SHBG levels are a suitable marker of increased pancreatic ß-cell demand.

  9. Anti-T-lymphocyte globulin-based non-myeloablative stem cell transplantation followed by HLA-identical donor lymphocyte infusion for hematologic malignancies

    Institute of Scientific and Technical Information of China (English)

    毛平; 王顺清; 王汉平; 李庆山; 许艳丽; 莫文健; 应逸; 朱志刚

    2003-01-01

    Objective To evaluate the application of anti-T-lymphocyte globulin (ATG) based nonmyeloablative but profoundly immunosuppressive regimens followed by donor lymphocyte infusion (DLI) for the treatment of hematologic malignancies.Methods The protocol was designed to minimize the intensity of the conditioning regimen to the range of nonmyeloablative therapies based on ATG with low-dose busulfan (Bu) and Cytoxan (CTX) (15-19.5 mg/kg, 8 mg/kg and 80 mg/kg, respectively). The patients received the first lymphocytic infusion from HLA-identical sibling donors on days 28-30 after transplant, and the first T cell dosage of 106/kg followed by the escalated dosage in the range of (0.5-1.5)×108/kg. The total number of procedures were performed at a median of 4.2 procedures (range of 2-8 procedures).Results Engraftment was documented in all six patients in the form of donor-recipient hematopoietic cells mixed chimera at early-stage posttransplant, which was converted gradually into complet chimera by DLI in four patients. Graft-versus-host disease (GVHD) developed in three of six cases, only one of which was severe. To date,four patients are disease free and alive.Conclusions Allogeneic donor stem cell engraftment into host can be achieved by nonmyeloablative conditioning regimen based on ATG. Transient mixed donor-recipient hematopoietic cell mixed with chimeras may be successfully converted into complete chimerism by DLI posttransplant. GVHD remains major clinical concern in our study.

  10. The effects of caffeinated and decaffeinated coffee on sex hormone-binding globulin and endogenous sex hormone levels: a randomized controlled trial

    Directory of Open Access Journals (Sweden)

    Wedick Nicole M

    2012-10-01

    Full Text Available Abstract Background Findings from observational studies suggest that sex hormone-binding globulin (SHBG and endogenous sex hormones may be mediators of the putative relation between coffee consumption and lower risk of type 2 diabetes. The objective of this study was to evaluate the effects of caffeinated and decaffeinated coffee on SHBG and sex hormone levels. Findings After a two-week run-in phase with caffeine abstention, we conducted an 8-week parallel-arm randomized controlled trial. Healthy adults (n = 42 were recruited from the Boston community who were regular coffee consumers, nonsmokers, and overweight. Participants were randomized to five 6-ounce cups of caffeinated or decaffeinated instant coffee or water (control group per day consumed with each meal, mid-morning, and mid-afternoon. The main outcome measures were SHBG and sex hormones [i.e., testosterone, estradiol, dehydroepiandrosterone sulfate]. No significant differences were found between treatment groups for any of the studied outcomes at week 8. At 4 weeks, decaffeinated coffee was associated with a borderline significant increase in SHBG in women, but not in men. At week 4, we also observed several differences in hormone concentrations between the treatment groups. Among men, consumption of caffeinated coffee increased total testosterone and decreased total and free estradiol. Among women, decaffeinated coffee decreased total and free testosterone and caffeinated coffee decreased total testosterone. Conclusions Our data do not indicate a consistent effect of caffeinated coffee consumption on SHBG in men or women, however results should be interpreted with caution given the small sample size. This is the first randomized trial investigating the effects of caffeinated and decaffeinated coffee on SHBG and sex hormones and our findings necessitate further examination in a larger intervention trial.

  11. Development of classification model and QSAR model for predicting binding affinity of endocrine disrupting chemicals to human sex hormone-binding globulin.

    Science.gov (United States)

    Liu, Huihui; Yang, Xianhai; Lu, Rui

    2016-08-01

    Disturbing the transport process is a crucial pathway for endocrine disrupting chemicals (EDCs) to disrupt endocrine function. However, this mechanism has not gotten enough attention, compared with that of hormone receptors and synthetase up to now, especially for the sex hormone transport process. In this study, we selected sex hormone-binding globulin (SHBG) and EDCs as a model system and the relative competing potency of a chemical with testosterone binding to SHBG (log RBA) as the endpoints, to develop classification models and quantitative structure-activity relationship (QSAR) models. With the classification model, a satisfactory model with nR09, nR10 and RDF155v as the most relevant variables was screened. Statistic results indicated that the model had the sensitivity, specificity, accuracy of 86.4%, 80.0%, 84.4% and 85.7%, 87.5%, 86.2% for the training set and validation set, respectively, highlighting a high classification performance of the model. With the QSAR model, a satisfactory model with statistical parameters, specifically, an adjusted determination coefficient (Radj(2)) of 0.810, a root mean square error (RMSE) of 0.616, a leave-one-out cross-validation squared correlation coefficient (QLOO(2)) of 0.777, a bootstrap method (QBOOT(2)) of 0.756, an external validation coefficient (Qext(2)) of 0.544 and a RMSEext of 0.859, were obtained, which implied satisfactory goodness of fit, robustness and predictive ability. The applicability domain of the current model covers a large number of structurally diverse chemicals, especially a few classes of nonsteroidal compounds. PMID:27156209

  12. 丙种球蛋白对白血病化疗者感染和输血的影响%Effect of gamma globulin on leukemia chemotherapy infection and blood transfusion

    Institute of Scientific and Technical Information of China (English)

    2015-01-01

    Objective To explore the effect of gamma globulin in acute leukemia (AL)of chemotherapy infection and blood transfusion.Methods According to application of gamma globulin,95 AL patients were divided into experimental group (36 cases )and control group (59 cases ).The infection,humoral immunity and blood transfusion were observed in two groups of patients.Results The immunoglobulin and complement level of two groups showed no significant difference.The experimental group showed significant decrease in anti-infection time,the number of platelet transfusion,adverse reaction of blood transfusion.Conclusion During AL chemotherapy,the application of gamma globulin is in favor of infection control,reduces support for treatment costs.%目的:探讨应用丙种球蛋白对急性白血病(AL)化疗者感染和输血的影响。方法95例 AL 患者根据是否应用丙种球蛋白分为实验组(36例)和对照组(59例)。观察对两组患者感染、体液免疫情况及输血的影响。结果两组免疫球蛋白和补体水平差异不大;实验组抗感染时间明显减少,输注血小板数量减少,输血不良反应率降低。结论在 AL 化疗期间应用丙种球蛋白利于感染控制,减少支持治疗费用。

  13. Extraction process and analysis amino acid composition of defatted rice bran albumin and globulin%脱脂米糠中清蛋白和球蛋白的提取工艺及氨基酸组成分析

    Institute of Scientific and Technical Information of China (English)

    王艳玲; 张敏

    2013-01-01

    Defatted rice bran was an important nutrition source of high quality whole plant protein. Among them, the content of albumin was the highest and globulin followed by. Extraction technology on albumin and globulin of rice bran was studied. The influence of different factors on the protein yield was discussed. Determined the optimal conditions of the process. And the composition of amino acid were measured. The result showed that the optimal processing parameters of albumin liquid ratio was 1:14,extraction time was 3h,temperature was 45℃,albumin yield could up to 14.68%. Globulin optimum parameters for NaCI density was 3%, liquid ratio was 1:10,extraction time was 2h. temperature was 50℃,globulin yield was 4.28%. The essential amino acid content in the two proteins was approached than that in the FAO/WHO recommended adult pattern. It was the good quality source of protein plant.%脱脂米糠是重要的优质全价植物蛋白质营养源,其蛋白质中清蛋白含量最高,球蛋白其次.对米糠中清蛋白和球蛋白提取工艺进行了研究,探讨不同工艺因素对蛋白得率的影响,确定最佳提取工艺,并对产品的氨基酸组成进行测定.研究表明,清蛋白最佳提取工艺为料液比1:14,提取时间3h,温度45℃,清蛋白得率可达14.64%;球蛋白最佳提取工艺为NaCl浓度为3%,料液比1∶10,提取时间2h,温度50℃,球蛋自得率为4.28%;两种蛋白质中必需氨基酸含量接近FAO/WHO推荐模式,是优质植物蛋白质来源.

  14. Effects of intraperitoneal insulin versus subcutaneous insulin administration on sex hormone-binding globulin concentrations in patients with type 1 diabetes mellitus

    Directory of Open Access Journals (Sweden)

    M Boering

    2016-06-01

    Full Text Available Aims Elevated sex hormone-binding globulin (SHBG concentrations have been described in patients with type 1 diabetes mellitus (T1DM, probably due to low portal insulin concentrations. We aimed to investigate whether the route of insulin administration, continuous intraperitoneal insulin infusion (CIPII, or subcutaneous (SC, influences SHBG concentrations among T1DM patients. Methods Post hoc analysis of SHBG in samples derived from a randomized, open-labeled crossover trial was carried out in 20 T1DM patients: 50% males, mean age 43 (±13 years, diabetes duration 23 (±11 years, and hemoglobin A1c (HbA1c 8.7 (±1.1 (72 (±12 mmol/mol. As secondary outcomes, testosterone, 17-β-estradiol, luteinizing hormone (LH, and follicle-stimulating hormone (FSH were analyzed. Results Estimated mean change in SHBG was −10.3nmol/L (95% CI: −17.4, −3.2 during CIPII and 3.7nmol/L (95% CI: −12.0, 4.6 during SC insulin treatment. Taking the effect of treatment order into account, the difference in SHBG between therapies was −6.6nmol/L (95% CI: −17.5, 4.3; −12.7nmol/L (95% CI: −25.1, −0.4 for males and −1.7nmol/L (95% CI: −24.6, 21.1 for females, respectively. Among males, SHBG and testosterone concentrations changed significantly during CIPII; −15.8nmol/L (95% CI: −24.2, −7.5 and −8.3nmol/L (95% CI: −14.4, −2.2, respectively. The difference between CIPII and SC insulin treatment was also significant for change in FSH 1.2U/L (95% CI: 0.1, 2.2 among males. Conclusions SHBG concentrations decreased significantly during CIPII treatment. Moreover, the difference in change between CIPII and SC insulin therapy was significant for SHBG and FSH among males. These findings support the hypothesis that portal insulin administration influences circulating SHBG and sex steroids.

  15. Effects of intraperitoneal insulin versus subcutaneous insulin administration on sex hormone-binding globulin concentrations in patients with type 1 diabetes mellitus

    Science.gov (United States)

    Boering, M; Logtenberg, S J J; Groenier, K H; Wolffenbuttel, B H R; Gans, R O B; Kleefstra, N; Bilo, H J G

    2016-01-01

    Aims Elevated sex hormone-binding globulin (SHBG) concentrations have been described in patients with type 1 diabetes mellitus (T1DM), probably due to low portal insulin concentrations. We aimed to investigate whether the route of insulin administration, continuous intraperitoneal insulin infusion (CIPII), or subcutaneous (SC), influences SHBG concentrations among T1DM patients. Methods Post hoc analysis of SHBG in samples derived from a randomized, open-labeled crossover trial was carried out in 20 T1DM patients: 50% males, mean age 43 (±13) years, diabetes duration 23 (±11) years, and hemoglobin A1c (HbA1c) 8.7 (±1.1) (72 (±12) mmol/mol). As secondary outcomes, testosterone, 17-β-estradiol, luteinizing hormone (LH), and follicle-stimulating hormone (FSH) were analyzed. Results Estimated mean change in SHBG was −10.3nmol/L (95% CI: −17.4, −3.2) during CIPII and 3.7nmol/L (95% CI: −12.0, 4.6) during SC insulin treatment. Taking the effect of treatment order into account, the difference in SHBG between therapies was −6.6nmol/L (95% CI: −17.5, 4.3); −12.7nmol/L (95% CI: −25.1, −0.4) for males and −1.7nmol/L (95% CI: −24.6, 21.1) for females, respectively. Among males, SHBG and testosterone concentrations changed significantly during CIPII; −15.8nmol/L (95% CI: −24.2, −7.5) and −8.3nmol/L (95% CI: −14.4, −2.2), respectively. The difference between CIPII and SC insulin treatment was also significant for change in FSH 1.2U/L (95% CI: 0.1, 2.2) among males. Conclusions SHBG concentrations decreased significantly during CIPII treatment. Moreover, the difference in change between CIPII and SC insulin therapy was significant for SHBG and FSH among males. These findings support the hypothesis that portal insulin administration influences circulating SHBG and sex steroids. PMID:27287189

  16. Testosterone, sex hormone-binding globulin and the metabolic syndrome in men: an individual participant data meta-analysis of observational studies.

    Directory of Open Access Journals (Sweden)

    Judith S Brand

    Full Text Available Low total testosterone (TT and sex hormone-binding globulin (SHBG concentrations have been associated with the metabolic syndrome (MetS in men, but the reported strength of association varies considerably.We aimed to investigate whether associations differ across specific subgroups (according to age and body mass index (BMI and individual MetS components.Two previously published meta-analyses including an updated systematic search in PubMed and EMBASE.Cross-sectional or prospective observational studies with data on TT and/or SHBG concentrations in combination with MetS in men.We conducted an individual participant data meta-analysis of 20 observational studies. Mixed effects models were used to assess cross-sectional and prospective associations of TT, SHBG and free testosterone (FT with MetS and its individual components. Multivariable adjusted odds ratios (ORs and hazard ratios (HRs were calculated and effect modification by age and BMI was studied.Men with low concentrations of TT, SHBG or FT were more likely to have prevalent MetS (ORs per quartile decrease were 1.69 (95% CI 1.60-1.77, 1.73 (95% CI 1.62-1.85 and 1.46 (95% CI 1.36-1.57 for TT, SHBG and FT, respectively and incident MetS (HRs per quartile decrease were 1.25 (95% CI 1.16-1.36, 1.44 (95% 1.30-1.60 and 1.14 (95% 1.01-1.28 for TT, SHBG and FT, respectively. Overall, the magnitude of associations was largest in non-overweight men and varied across individual components: stronger associations were observed with hypertriglyceridemia, abdominal obesity and hyperglycaemia and associations were weakest for hypertension.Associations of testosterone and SHBG with MetS vary according to BMI and individual MetS components. These findings provide further insights into the pathophysiological mechanisms linking low testosterone and SHBG concentrations to cardiometabolic risk.

  17. Improved Outcome of a Reduced Toxicity-Fludarabine, Cyclophosphamide, plus Antithymocyte Globulin Conditioning Regimen for Unrelated Donor Transplantation in Severe Aplastic Anemia: Comparison of 2 Multicenter Prospective Studies.

    Science.gov (United States)

    Kang, Hyoung Jin; Hong, Kyung Taek; Lee, Ji Won; Kim, Hyery; Park, Kyung Duk; Shin, Hee Young; Lee, Soo Hyun; Yoo, Keon Hee; Sung, Ki Woong; Koo, Hong Hoe; Lee, Jae Wook; Chung, Nak Gyun; Cho, Bin; Kim, Hack Ki; Koh, Kyung Nam; Im, Ho Joon; Seo, Jong Jin; Jung, Hyun Joo; Park, Jun Eun; Lee, Young Ho; Lim, Young Tak; Lim, Yeon Jung; Kim, Sun Young; Yoo, Eun Sun; Ryu, Kyung Ha; Lee, Jae Hee; Park, Jeong-A; Park, Sang Kyu; Ahn, Hyo Seop

    2016-08-01

    Hematopoietic stem cell transplantation (HSCT) is a curative therapy for severe aplastic anemia (SAA); however, the optimal conditioning regimen for HSCT with an unrelated donor has not yet been defined. A previous study using a fludarabine (FLU), cyclophosphamide (Cy), and antithymocyte globulin (ATG) conditioning regimen (study A: 50 mg/kg Cy once daily i.v. on days -9, -8, -7, and -6; 30 mg/m(2) FLU once daily i.v. on days -5, -4, -3, and -2; and 2.5 mg/kg of ATG once daily i.v. on days -3, -2, and -1) demonstrated successful engraftment (100%) but had a high treatment-related mortality rate (32.1%). Therefore, given that Cy is more toxic than FLU, we performed a new phase II prospective study with a reduced-toxicity regimen (study B: 60 mg/kg Cy once daily i.v. on days -8 and -7; 40 mg/m(2) FLU once daily i.v. on days -6, -5, -4, -3, and -2; and 2.5 mg/kg ATG once daily i.v. on 3 days). Fifty-seven patients were enrolled in studies A (n = 28) and B (n = 29), and donor type hematologic recovery was achieved in all patients in both studies. The overall survival (OS) and event-free survival (EFS) rates of patients in study B was markedly improved compared with those in study A (OS: 96.7% versus 67.9%, respectively, P = .004; EFS: 93.3% versus 64.3%, respectively, P = .008). These data show that a reduced-toxicity conditioning regimen with FLU, Cy, and ATG may be an optimal regimen for SAA patients receiving unrelated donor HSCT. PMID:27090956

  18. Tumor uptake of radioiodinated anti-human pulmonary surfactant-associated protein monoclonal antibody PE 10 in nude mice bearing human pulmonary adenocarcinoma in combination with an unlabeled preload

    International Nuclear Information System (INIS)

    This study assessed the potential use of radioimmunoscintigraphy of pulmonary alveolar Type II cells tumor with the radiolabeled anti-human surfactant-associated protein (SP) monoclonal antibody (MAb) PE 10 in combination with preloads of unlabeled MAb. The in vitro binding of iodine-125 (125I)-labeled MAb PE 10 (1 μg), which had a specific radioactivity of 400 MBq/mg, on human pulmonary papillary adenocarcinoma NCI-H441 cells that produced SP was investigated. In NCI-H441 tumor-bearing nude mice, the tumor uptake of 125I-MAb PE 10 (5 μg) was examined in combination with preloads of unlabeled MAb PE 10 (0, 5, 10, and 50 μg). An isotype-matched unassociated murine MAb was used as a control both in vitro and in vivo. 125I-MAb PE 10 showed specific cell binding compared with 125I-control MAb. Tumor uptake of 125I-MAb PE 10 in vivo reached a peak of 4.97±0.33% injected dose per gram (%ID/g) at 48 h postinjection. Preloads of 5 and 10 μg unlabeled MAb PE 10 significantly enhanced tumor uptake at 48 h postinjection ( 5.94±0.29% ID/g and 5.72±0.29% ID/g, respectively), whereas preload of 50 μg unlabeled MAb PE 10 significantly decreased tumor uptake ( 2.75±0.32% ID/g) at 48 h. Preload of 5 μg unlabeled MAb PE 10 significantly increased the tumor-to-blood radioactivity ratio at 48 h ( 2.39±0.16). Preloads of unlabeled control MAb did not cause any significant change in tumor uptake. Immunohistochemistry showed the intracellular and pericellular patterns of SP expression in tumor cells. In conclusion, radioimmunoscintigraphy with MAb PE 10 labeled with a γ-emitting radioiodine such as 123I might be a useful means of targeting pulmonary alveolar Type II tumor cells in combination with preloading with an optimal dose of the unlabeled MAb

  19. A genome-wide association meta-analysis of circulating sex hormone-binding globulin reveals multiple Loci implicated in sex steroid hormone regulation.

    Directory of Open Access Journals (Sweden)

    Andrea D Coviello

    Full Text Available Sex hormone-binding globulin (SHBG is a glycoprotein responsible for the transport and biologic availability of sex steroid hormones, primarily testosterone and estradiol. SHBG has been associated with chronic diseases including type 2 diabetes (T2D and with hormone-sensitive cancers such as breast and prostate cancer. We performed a genome-wide association study (GWAS meta-analysis of 21,791 individuals from 10 epidemiologic studies and validated these findings in 7,046 individuals in an additional six studies. We identified twelve genomic regions (SNPs associated with circulating SHBG concentrations. Loci near the identified SNPs included SHBG (rs12150660, 17p13.1, p = 1.8 × 10(-106, PRMT6 (rs17496332, 1p13.3, p = 1.4 × 10(-11, GCKR (rs780093, 2p23.3, p = 2.2 × 10(-16, ZBTB10 (rs440837, 8q21.13, p = 3.4 × 10(-09, JMJD1C (rs7910927, 10q21.3, p = 6.1 × 10(-35, SLCO1B1 (rs4149056, 12p12.1, p = 1.9 × 10(-08, NR2F2 (rs8023580, 15q26.2, p = 8.3 × 10(-12, ZNF652 (rs2411984, 17q21.32, p = 3.5 × 10(-14, TDGF3 (rs1573036, Xq22.3, p = 4.1 × 10(-14, LHCGR (rs10454142, 2p16.3, p = 1.3 × 10(-07, BAIAP2L1 (rs3779195, 7q21.3, p = 2.7 × 10(-08, and UGT2B15 (rs293428, 4q13.2, p = 5.5 × 10(-06. These genes encompass multiple biologic pathways, including hepatic function, lipid metabolism, carbohydrate metabolism and T2D, androgen and estrogen receptor function, epigenetic effects, and the biology of sex steroid hormone-responsive cancers including breast and prostate cancer. We found evidence of sex-differentiated genetic influences on SHBG. In a sex-specific GWAS, the loci 4q13.2-UGT2B15 was significant in men only (men p = 2.5 × 10(-08, women p = 0.66, heterogeneity p = 0.003. Additionally, three loci showed strong sex-differentiated effects: 17p13.1-SHBG and Xq22.3-TDGF3 were stronger in men, whereas 8q21.12-ZBTB10 was stronger in women. Conditional analyses identified additional signals at the SHBG gene that together almost double the proportion

  20. The treatment effect observation of intravenous gamma globulin for in-fectious mononucleosis%静注丙种球蛋白治疗传染性单核细胞增多症的疗效观察

    Institute of Scientific and Technical Information of China (English)

    何洲; 彭升; 古超群

    2015-01-01

    目的:探讨静注丙种球蛋白(IVIG)治疗传染性单核细胞增多症的临床疗效。方法选择2013年9月~2014年8月我院接收的传染性单核细胞增多症患者80例,随机分为观察组和对照组,各40例。观察组应用静注丙种球蛋白进行治疗,而对照组则使用病毒唑加IFN-α(干扰素-α)进行治疗,对比分析两组的有效率、临床症状及生命体征的变化。结果观察组患者的临床症状、生命体征及治愈率均显著优于对照组(P<0.05)。结论使用静注丙种球蛋白治疗传染性单核细胞增多症疗效确切,安全性强,值得临床推广和运用。%Objective To investigate the clinical effect of intravenous injection of gamma globulin in the treatment of infectious mononucleosis. Methods All 80 cases of child patients with infectious mononucleosis from September 2013 to August 2014 in our hospital were randomly divided into the observation group and control group ,each group included 40 cases. The observation group was treated with gamma globulin , while the control group were given ribavirin plus IFN-α treatment, the efficiency, the clinical symptoms and signs of life changes were compared between two groups. Results The clinical symptom,life signs and the cure rate of observation group were significantly better than the control group (P<0.05). Conclusion Gamma globulin in treatment of infectious mononucleosis has define effect and safety, worthy of clinical promotion and application.

  1. Prevención de la isoinmunización materna al RhD, con gamma-globulina anti-D Prevention of maternal RhD isoimmunization with anti-D gamma globulin

    Directory of Open Access Journals (Sweden)

    Héctor Alfredo Baptista-González

    2001-02-01

    Full Text Available OBJETIVO: Presentar la experiencia institucional en la prevención de la isoinmunización al RhD, mediante el empleo de 150 µg de gamma-globulina anti-D en las mujeres Rh negativo. MATERIAL Y MÉTODOS: Se registraron los antecedentes inmunohematológicos de los casos consecutivos de todas las mujeres Rh negativo que acudieron, para su atención médica, al Instituto Nacional de Perinatología entre 1982 y 1995. A las mujeres con riesgo de isoinmunización se les aplicó 150 µg de gamma-globulina anti-D, con fines preventivos. RESULTADOS: En el periodo de estudio ingresaron 4 857 mujeres Rh negativo (4.85% del total de mujeres, de las cuales 629 (13.0%, presentaron isoinmunización al RhD. De estas últimas, 542 (86.2% ya se encontraron isoinmunizadas desde antes de su ingreso al Instituto. En 22 casos (3.5%, la isoinmunización ocurrió a pesar de que recibieron la dosis adecuada de gamma-globulina anti D. De las 2 605 pacientes (53.6% sometidas a prevención, a 2 039 se les aplicó una sola dosis, y a 475, hasta dos dosis. En 22 casos se documentó la falla de la prevención; sin embargo, en cuatro de ellos, se registraron embarazos múltiples, y los restantes 18 presentaron patología obstétrica asociada. CONCLUSIONES: Mediante este programa de prevención, consistente en administrar 150 µg de gamma-globulina anti-D por dosis, es posible reducir la isoinmunización a menos de un caso por cada 1 000 mujeres. Los fracasos en la prevención de la isoinmunización se asociaron a condiciones obstétricas agregadas y al incumplimiento de las guías o lineamientos del programa. El texto completo en inglés de este artículo está disponible en: http://www.insp.mx/salud/index.htmlOBJECTIVE: To report our experience in preventing RhD maternal isoimmunization by using anti-D gamma globulin among Rh-negative women. MATERIAL AND METHODS: Between 1982 and 1995, immunologic and hematologic data were collected from all Rh-negative women seen at Mexico

  2. B-cell-rich T-cell lymphoma associated with Epstein-Barr virus-reactivation and T-cell suppression following antithymocyte globulin therapy in a patient with severe aplastic anemia

    Directory of Open Access Journals (Sweden)

    Nobuyoshi Hanaoka

    2015-09-01

    Full Text Available B-cell lymphoproliferative disorder (B-LPD is generally characterized by the proliferation of Epstein-Barr virus (EBV-infected B lymphocytes. We here report the development of EBV-negative B-LPD associated with EBV-reactivation following antithymocyte globulin (ATG therapy in a patient with aplastic anemia. The molecular autopsy study showed the sparse EBV-infected clonal T cells could be critically involved in the pathogenesis of EBV-negative oligoclonal B-LPD through cytokine amplification and escape from T-cell surveillances attributable to ATG-based immunosuppressive therapy, leading to an extremely rare B-cell-rich T-cell lymphoma. This report helps in elucidating the complex pathophysiology of intractable B-LPD refractory to rituximab.

  3. Sex hormone binding globulin decrease as potential pathogenetic factor for hirsutism in adolescent girls Disminución de la globulina transportadora de hormonas sexuales como factor patogénico de hirsutismo en la adolescencia

    Directory of Open Access Journals (Sweden)

    Graciela Cross

    2008-04-01

    Full Text Available We investigated 252 non-obese female subjects aged 13-39 years to evaluate if an exaggerated descent of sex hormone binding globulin (SHBG levels during adolescence can play a role in the development of hirsutism. Body hair was assessed according to Ferriman and Gallwey (FG, with a stringent criterion of normality of 4 and controls (FG Se investigaron 252 mujeres con peso normal, de 13 a 39 años de edad, para evaluar si un descenso exagerado en los niveles de la globulina transportadora de hormonas sexuales ("sex hormone binding globulin"; SHBG puede tener un rol en el desarrollo de hirsutismo. Este signo fue evaluado con la escala de Ferriman y Gallwey (FG, empleando un criterio riguroso de normalidad 4 y controles (FG < 4, ciclos menstruales regulares, sin acné. En adolescentes de 15-18 años, los valores de SHBG fueron menores en las "hirsutas", los niveles de FT fueron similares en ambos grupos y el índice de FG correlacionó inversamente con SHBG. En las mujeres de 19-39 años, los niveles de FT fueron mayores en las "hirsutas", los valores de SHBG fueron similares en ambos grupos y FG correlacionó positivamente con FT. Los valores más bajos de SHBG se observaron entre 15 y 18 años, pero la pendiente de disminución a partir de los valores de 13-14 años fue mayor en el grupo de "hirsutas". Los valores de FT se incrementaron progresivamente con la edad, pero el aumento fue mayor en el grupo de "hirsutas". Estos resultados sugieren un rol importante del descenso de SHBG en la adolescencia vs. un incremento más acentuado de los niveles de testosterona en las adultas, como factores que condicionan el desarrollo del hirsutismo en esos dos diferentes periodos de la vida.

  4. 7S球蛋白水解物对猪肉糜凝胶的影响%Effect of Alcalase Hydrolysates of Soybean 7S Globulin on Gel Properties of Minced Pork

    Institute of Scientific and Technical Information of China (English)

    段春红; 潘思轶

    2012-01-01

    In this study, the influence of soybean 7S globulin hydrolysates with different degree of hydrolysis (DH) on gel properties of minced pork was investigated. Soybean 7S globulin was first hydrolyzed to different DH (3%, 6%, 9%, 12%, 15%) using alcalase. Hydrolysates were then added to minced pork to make pork sausage. Interactions to stabilize the gel structure of pork sausage were finally analyzed to determine the influence of hydrolysates on gel properties of minced pork. The results obtained showed that hydrophobic interaction was not significantly related to the gel strength of pork sausage, indicating it was not a principle interaction in maintaining the gel network of mined pork. While, disulfide bond and hydrophobic interactions were significantly related to the springiness and chewiness of pork sausage (P 〈 0.01). Hydrogen bond was also significantly related to springiness of the pork sausage (P 〈 0.05), indicating that disulfide bonds, hydrophobic interactions and hydrogen bonds were the principle forces to maintain pork gel network. Scanning electron microscopy (SEM) revealed that hydrolysates of soybean 7S globulin improved the gel properties of minced pork. With increasing DH added hydrolysates, the pork gel became more compact and uniform, with no noticeable pores in it.%以大豆为原料,提取7s球蛋白,并采用碱性蛋白酶对其进行限制性酶解,控制其水解度,得到水解度为3%、6%、9%、12%、15%的酶解产物,将酶解产物与猪肉糜混合制备猪肉肠,分析添加不同水解度的酶解产物所形成的猪肉糜凝胶的化学作用力的变化。结果表明:静电作用与猪肉糜凝胶强度相关性不显著,不是维持猪肉糜凝胶网络结构的主要作用力;疏水作用与猪肉糜凝胶的回复性和咀嚼性呈现极显著的正相关性;氢键与猪肉糜凝胶的弹性呈现极显著的正相关性,二硫键与猪肉糜凝胶的弹性、内聚性

  5. 静滴"人废血丙种球蛋白"在小儿外科感染中的应用%γ-Globulin from Human Blood Used in Treatment of Infectious Diseases in Pediatric Surgery

    Institute of Scientific and Technical Information of China (English)

    戴约; 谷兴琳

    1986-01-01

    @@ 我院自1978年2月至1980年10月,应用南京中心血库配制的"人废血丙种球旦白"(以下简称"丙球"),静脉点滴治疗和预防小儿外科严重感染性疾病90例,收到良好效果,现报告如下: 临床资料%This paper sums up our practice in the treatment of 90 cases of serious infectious diseases with r-globulin derived from waste human blood, with a Success rate of 89.3%. 205 intravenous infusions by drip were given in this group. In one case, urticaria occurred 2 hrs after the infusion and soon disappeared after administration of Toldrin. The patients had chills and high fever during the first infusion but none after the second. The rest of them showed no sign of side effect. After treatment, their IgA, IgM and IgG got elevated 40 various degrees.

  6. Radioimmunoassay of ''free thyroxin'' in dried blood spots on filter paper - preliminary observations on the effective differentiation of subjects with congenital hypothyroidism from those with subnormal thyroxin-binding globulin and normal subjects

    International Nuclear Information System (INIS)

    In this sensitive, simple method for measuring ''free thyroxin'' (FT4) in eluates of dried blood spots on filter paper by use of a radioimmunoassay kit (Amerlex Free T4 RIA), the measurable range of FT4 is 1.8 to 57 ng/L (equivalent to the concentration in serum), or 7 to 237 fg/tube. The mean coefficients of variation for within assay-within spots, within assay-between spots, and between assays were 5.3%, 5.0%, and 6.2%, respectively. FT4 in blood spotted on filter paper is stable for at least a month when dried and kept at either -200C, 40C, room temperature (about 250C), or 370C. The results for FT4 in dried blood spots correlated closely with the free-T4 concentration in serum (r = 0.99). The method can be used to differentiate cases of primary and secondary hypothyroidism from normal subjects and those with subnormal thyroxin-binding globulin. This method may be useful in screening for congenital hypothyroidism, because sample-retesting is not necessary

  7. Gamma globulin, Evan's blue, aprotinin A PLA2 inhibitor, tetracycline and antioxidants protect epithelial cells against damage induced by synergism among streptococcal hemolysins, oxidants and proteinases: relation to the prevention of post-streptococcal sequelae and septic shock.

    Science.gov (United States)

    Ginsburg, I; Sadovnic, M

    1998-11-01

    An in vitro model was employed to study the potential role of streptococcal extra-cellular products, rich in streptolysin O, in cellular injury as related to streptococcal infections and post-streptococcal sequelae. Extra-cellular products (EXPA) rich in streptolysin O were isolated from type 4, group A hemolytic streptococci grown in a chemostat, in a synthetic medium. EXPA induced moderate cytopathogenic changes in monkey kidney epithelial cells and in rat heart cells pre-labeled with 3H-arachidonate. However very strong toxic effects were induced when EXP was combined with oxidants (glucose oxides generated H2O2, AAPH-induced peroxyl radical (ROO.), NO generated by sodium nitroprusside) and proteinases (plasmin, trypsin). Cell killing was distinctly synergistic in nature. Cell damage induced by the multi-component cocktails was strongly inhibited either by micromolar amounts of gamma globulin, and Evan's blue which neutralized SLO activity, by tetracycline, trasylol (aprotinin), epsilon amino caproic acid and by soybean trypsin inhibitor, all proteinase inhibitors as well as by a non-penetrating PLA2 inhibitor A. The results suggest that fasciitis, myositis and sepsis resulting from infections with hemolytic streptococci might be caused by a coordinated 'cross-talk' among microbial, leukocyte and additional host-derived pro-inflammatory agents. Since attempts to prolong lives of septic patients by the exclusive administration of single antagonists invariably failed, it is proposed that the administration of 'cocktails' of putative inhibitors against major pro-inflammatory agonizes generated in inflammation and infection might protect against the deleterious effects caused by the biochemical and pharmacological cascades which are known to be activated in sepsis. PMID:9848686

  8. Sex hormone-binding globulin, type 2 diabetes and obesity%性激素结合球蛋白与2型糖尿病及肥胖

    Institute of Scientific and Technical Information of China (English)

    张敏; 闫丽丽; 王玉环

    2011-01-01

    Sex hormone binding globulin (SHBG) is a glycoprotein secreted by the liver, which mediates and (or) activates adenylate cyclase system directly through binding with peculiar SHBG receptor in surface of organic cells, thereby regulates cell function. Many studies have explored that, SHBG is related with type 2 diabetes and obesity, and could reflect the extent of insulin resistance in type 2 diabetic patients with obesity. The lower level of SHBG could increase the risk of insulin resistance and type 2 diabetes, and lead to central obesity. Higher serum SHBG reduces diseases such as type 2 diabetes and obesity through regulating fasting blood glucose, but not through improving insulin secretion and androgen mechanism.%性激素结合球蛋白(SHBG)是由肝脏产生的一种糖蛋白,通过与组织细胞表面的特异性受体结合介导和(或)直接激活腺苷酸环化酶系统,从而调节组织细胞功能.研究显示,SHBG与2型糖尿病和肥胖相关,可反映肥胖2型糖尿病患者的胰岛素抵抗程度.低SHBG水平可增加胰岛素抵抗及2型糖尿病的发病风险,可导致腹型肥胖.高血清SHBG通过调整空腹血糖,而不是通过改善胰岛素分泌或雄激素机制,延缓糖尿病及肥胖等疾病的发生.

  9. Investigation of genetic variants, birthweight and hypothalamic-pituitary-adrenal axis function suggests a genetic variant in the SERPINA6 gene is associated with corticosteroid binding globulin in the western Australia pregnancy cohort (Raine study.

    Directory of Open Access Journals (Sweden)

    Laura N Anderson

    Full Text Available The hypothalamic-pituitary-adrenal (HPA axis regulates stress responses and HPA dysfunction has been associated with several chronic diseases. Low birthweight may be associated with HPA dysfunction in later life, yet human studies are inconclusive. The primary study aim was to identify genetic variants associated with HPA axis function. A secondary aim was to evaluate if these variants modify the association between birthweight and HPA axis function in adolescents.Morning fasted blood samples were collected from children of the Western Australia Pregnancy Cohort (Raine at age 17 (n = 1077. Basal HPA axis function was assessed by total cortisol, corticosteroid binding globulin (CBG, and adrenocorticotropic hormone (ACTH. The associations between 124 tag single nucleotide polymorphisms (SNPs within 16 HPA pathway candidate genes and each hormone were evaluated using multivariate linear regression and penalized linear regression analysis using the HyperLasso method.The penalized regression analysis revealed one candidate gene SNP, rs11621961 in the CBG encoding gene (SERPINA6, significantly associated with total cortisol and CBG. No other candidate gene SNPs were significant after applying the penalty or adjusting for multiple comparisons; however, several SNPs approached significance. For example, rs907621 (p = 0.002 and rs3846326 (p = 0.003 in the mineralocorticoid receptor gene (NR3C2 were associated with ACTH and SERPINA6 SNPs rs941601 (p = 0.004 and rs11622665 (p = 0.008, were associated with CBG. To further investigate our findings for SERPINA6, rare and common SNPs in the gene were imputed from the 1,000 genomes data and 8 SNPs across the gene were significantly associated with CBG levels after adjustment for multiple comparisons. Birthweight was not associated with any HPA outcome, and none of the gene-birthweight interactions were significant after adjustment for multiple comparisons.Our study suggests that genetic variation in the SERPINA

  10. National Institutes of Health classification for chronic graft-versus-host disease predicts outcome of allo-hematopoietic stem cell transplant after fludarabine-busulfan-antithymocyte globulin conditioning regimen.

    Science.gov (United States)

    Saillard, Colombe; Crocchiolo, Roberto; Furst, Sabine; El-Cheikh, Jean; Castagna, Luca; Signori, Alessio; Oudin, Claire; Faucher, Catherine; Lemarie, Claude; Chabannon, Christian; Granata, Angela; Blaise, Didier

    2014-05-01

    Abstract In 2005, the National Institutes of Health (NIH) proposed standard criteria for diagnosis, organ scoring and global assessment of chronic graft-versus-host disease (cGvHD) severity. We retrospectively reclassified cGvHD with NIH criteria in a monocentric cohort of 130 consecutive adult patients with hematological malignancies presenting cGvHD after receiving allo-hematopoietic stem cell transplant (HSCT) with a fludarabine-busulfan-antithymocyte globulin (ATG) conditioning regimen, among 313 consecutive HSCT recipients. We compared NIH and Seattle classifications to correlate severity and outcome. The follow up range was effectively 2-120 months. Forty-four percent developed Seattle-defined cGvHD (22% limited, 78% extensive forms). Using NIH criteria, there were 23%, 40% and 37% mild, moderate and severe forms, respectively, and 58%, 32% and 8% classic cGvHD, late acute GvHD and overlap syndrome. Five-year overall survival was 55% (49-61), and cumulative incidences of non-relapse mortality (NRM) and relapse/progression at 2 years were 19% (14-23) and 19% (14-24). NIH mild and moderate forms were associated with better survival compared to severe cGvHD (hazard ratio [HR] = 3.28, 95% confidence interval [CI]: 1.38-7.82, p = 0.007), due to higher NRM among patients with severe cGvHD (HR = 3.04, 95% CI: 1.05-8.78, p = 0.04) but comparable relapse risk (p = NS). In conclusion, the NIH classification appears to be more accurate in predicting outcome mostly by the reclassification of old-defined extensive forms into NIH-defined moderate or severe.

  11. Isolamento da globulina majoritária, digestibilidade in vivo e in vitro das proteínas do tremoço-doce (Lupinus albus L., var. Multolupa Isolation of major globulin, in vivo and in vitro digestibility of proteins from sweet lupin (Lupinus albus L. var. Multolupa

    Directory of Open Access Journals (Sweden)

    Valdir Augusto Neves

    2006-12-01

    Full Text Available Os objetivos do trabalho foram isolar, purificar e estudar algumas propriedades da fração globulina majoritária de tremoço-doce, var. Multolupa; assim como avaliar as características de digestibilidade da farinha e frações isoladas. As frações protéicas foram separadas por fracionamento diferencial com uso de diferentes solventes. A globulina majoritária de tremoço-doce foi isolada, purificada por cromatografia em Q-Sepharose, revelando um único pico de proteína. Apresentou um peso molecular de 162,5 ± 10,0 kDa, determinado por cromatografia em Sephacryl S-300, e subunidades entre 20-70 kDa em PAGE-SDS. A solubilidade em função do pH e concentrações de NaCl revelaram curva típica dessa fração. A digestibilidade da proteína da farinha e das frações albumina, globulina e glutelina foi avaliada por experimentos in vitro e in vivo e se revelou alta para a fração globulina, seguida pela glutelina, albumina e farinha. A digestibilidade in vivo da fração globulina, tanto aparente quanto verdadeira, não diferiu significativamente daquela determinada para a caseína. Apesar da alta digestibilidade da fração majoritária e frações protéicas, a utilização como única fonte de proteína nas dietas revelou valores baixos de RPLR (razão protéica líquida relativa, indicando ser insuficiente para sustentar o crescimento dos animais, comparativamente à caseína.The aim of the present work was to isolate, purify and study some properties of the major globulin fraction from sweet lupin, var. Multolupa; as well as to evaluate the digestibility features of flour and isolated fractions. The protein fractions were separated by differential fractionation with different solvents. The lupin major globulin was isolated, purified by Q-Sepharose chromatography when it showed only one protein peak. The major protein presented a molecular weight of 162.5 ± 10.0 kDa, determined on Sephacryl S-300 chromatography; and subunits between 20

  12. Analysis of the relationship between prognosis and serum immune globulin and complement C3 contents change of patients with esophageal cancer after radiotherapy%食管癌放疗后免疫球蛋白、补体C3水平的变化与预后分析

    Institute of Scientific and Technical Information of China (English)

    李晶; 李博

    2013-01-01

    Objective To discuss the relationship between prognosis and serum immune globulin and complement C3 contents change of patients with esophageal cancer after radiotherapy. Methods By the methods of nephelometry.the serum immune globulin and complement C3 contents before and after radiotherapy of 41 patients with esophageal cancer were measured. Results The levels of all indexes were higher in the patients who survived more than 1 year than those died in 6 months or in 1 year. There was a significant statistic difference(P <0. 05). Conclusion The levels of serum immune globulin and complement C3 contents have observably correlation with prognosis.%目的 探讨食管癌患者放疗前后血清免疫球蛋白、补体C3水平的变化与放疗预后的关系.方法 采用散射比浊法对41例食管癌患者放疗前后进行血清免疫球蛋白及补体C3测定.结果 放疗后生存期>1年患者的各项免疫指标均高于6个月内死亡和1年内死亡患者,差异具有统计学意义(P<0.05).结论 食管癌患者的血清免疫球蛋白、补体C3的指标变化与其预后明显相关.

  13. Caracterização e hidrólise in vitro da globulina principal de grão-de-bico (Cicer arietinum L., var. IAC-Marrocos Characterization and in vitro tryptic hydrolysis of the major globulin from chickpea (Cicer arietinum L.

    Directory of Open Access Journals (Sweden)

    Valdir A. Neves

    2004-03-01

    Full Text Available No presente estudo procedeu-se ao isolamento e caracterização da fração globulina majoritária (11 S de grão-de-bico, var. IAC-Marrocos. A globulina majoritária extraída foi isolada por cromatografia de filtração em gel e de troca-iônica mostrando apenas uma banda de proteína na eletroforese em gel de poliacrilamida. A globulina majoritária, após passagem em coluna de Sephadex, revelou duas bandas protéicas de 55 e 52,5kDa e três bandas menores em gel de poliacrilamida dodecilsulfato de sódio. Na presença de 2-mercaptoetanol 6 polipeptídios na faixa de 18 a 42kDa foram revelados na eletroforese. A globulina isolada foi submetida à ação da tripsina e quimotripsina onde a forma nativa mostrou-se resistente à ação enzimática enquanto o aquecimento (96 e 121°C/15min não foi suficiente para aumentar a susceptibilidade à hidrólise, significativamente. Adição de NaCl 0,3M levou a um aumento da estabilidade estrutural com menor susceptibilidade à digestão proteolítica, fato em parte perdido com o aquecimento. As hidrólises foram acompanhadas por eletroforese em gel de poliacrilamida dodecilsulfato de sódio.The isolation and characterization of the major globulin fraction (11 S from Chickpea, vc IAC-Marrocos, were evaluated. The major globulin was extracted, isolated by gel filtration and ion-exchange chromatography showing only one protein band on PAGE. The globulin, after Sephadex elution, revealed two protein bands of 55 and 52.5kDa and three minor bands on SDS-PAGE. In the presence of 2-mercaptoethanol six polypeptides were revealed on SDS-PAGE in the range of 18 to 42kDa. The isolated native globulin shown to be resistant to trypsin and chymotrypsin however heating at 96 and 121ºC/15min was not sufficient to increase the hydrolysis significantly. The proteolytic susceptibility of the enzymes was reduced by 0.3M NaCl addition at the assay. The salt concentration was sufficient to stabilize the native protein

  14. Total body irradiation and cyclophosphamide plus antithymocyte globulin regimen is well tolerated and promotes stable engraftment as a preparative regimen before T cell-replete haploidentical transplantation for acute leukemia.

    Science.gov (United States)

    Fu, Haixia; Xu, Lanping; Liu, Daihong; Liu, Kaiyan; Zhang, Xiaohui; Chen, Huan; Chen, Yuhong; Han, Wei; Wang, Yu; Wang, Jingzhi; Wang, Fengrong; Huang, Xiaojun

    2014-08-01

    We compared total body irradiation (TBI, 700 cGy)/cyclophosphamide (Cy, 3.6 g/m(2))/simustine (250 mg/m(2)) plus antithymocyte globulin (ATG) (TBI/Cy plus ATG) with cytarabine (8 g/m(2))/i.v. busulfan (Bu, 9.6 mg/kg)/Cy (3.6 g/m(2))/simustine (250 mg/m(2)) plus ATG (modified Bu/Cy plus ATG) as preparative therapy in T cell-replete haploidentical hematopoietic stem cell transplantation (haplo-HSCT) for acute leukemia. From August 2009 to August 2013, 38 consecutive patients using TBI/Cy plus ATG regimen for T cell-replete haplo-HSCT (TBI group) at our center were eligible, which contained 28 high-risk and 10 standard-risk patients. A nested case-control study was designed. Seventy-seven patients using modified Bu/Cy plus ATG regimen (Bu group) were randomly selected in a 1 to 3:1 ratio matching for age, disease and status, year of HSCT (±2 years), and length of follow-up. Only 1 graft failure occurred in the TBI group. The incidence and time of neutrophil and platelet engraftment were comparable between the 2 groups. Severe grades III/IV graft-versus-host disease was observed in 13.4% of Bu group and only 2.6% of TBI group (P = .083). More toxicity of the liver (37.7% versus 10.5%; P = .002) and more hemorrhagic cystitis occurred in the Bu group (49.3% versus 23.7%, P = .008). Diarrhea was more common in the TBI group (44.7% versus 22.1%; P = .031). No significant differences were found in the 2-year incidences of relapse (26.5% for TBI group versus 32.3% for Bu group, P = .742), 1-year transplant-related mortality (12.6% versus 16.2%, P = .862), 2-year overall survival (60.2% versus 57.0%, P = .937), and 2-year incidence of disease-free survival (57.9% versus 56.6%, P = .845) between the 2 groups. We conclude that the TBI/Cy plus ATG regimen seems to be feasible in T cell-replete haplo-HSCT, which promotes stable engraftment and a lower incidence of liver toxicity and hemorrhagic cystitis. However, longer follow-up is necessary to

  15. Immune Globulin Intravenous: Myasthenia Gravis (Acute Exacerbation).

    Science.gov (United States)

    Generali, Joyce A; Cada, Dennis J

    2015-10-01

    This Hospital Pharmacy feature is extracted from Off-Label Drug Facts, a publication available from Wolters Kluwer Health. Off-Label Drug Facts is a practitioner-oriented resource for information about specific drug uses that are unapproved by the US Food and Drug Administration. This new guide to the literature enables the health care professional or clinician to quickly identify published studies on off-label uses and determine if a specific use is rational in a patient care scenario. References direct the reader to the full literature for more comprehensive information before patient care decisions are made. Direct questions or comments regarding Off-Label Drug Uses to jgeneral@ku.edu. PMID:26912917

  16. The sexuality assemblage: Desire, affect, anti-humanism

    OpenAIRE

    Fox, NJ; Alldred, P

    2013-01-01

    Two theoretical moves are required to resist the ‘humanist enticements’ associated with sexuality. Post-structuralism supplies the first, showing how the social produces culturally-specific sexual knowledgeabilities. A second anti-humanist move is then needed to overturn anthropocentric privileging of the human body and subject as the locus of sexuality. In this paper we establish a language and landscape for a Deleuze inspired anti-humanist sociology of sexuality that shifts the location of ...

  17. 一株识别肿瘤细胞上CD40突变体分子的单克隆抗体的研制及其生物学功能研究%Preparation and characterization of a mouse anti-human CD40 mutant monoclonal antibody

    Institute of Scientific and Technical Information of China (English)

    郑舒丹; 马泓冰; 高超; 汪家敏; 孙静; 罗先富; 张学光

    2009-01-01

    AIM: To prepare and characterize a mouse anti-human CD40 mutant monoclonal mAb. METHODS: Female BALB/c mice of 6-8 weeks old were immunized with CD40 mutant transfectant (L929-CD40mu) as immunogen. The spleen B cells of the mice were fused with Sp2/0 myeloma cells. The hybridoma cells were screened with CD40 mutant transfectant (L929-CD40mu) by FCM. Faststrip analysis was performed to identify Ig subclass of this mAb. The epitope recognized by this mAb was detected by Bio-5C11 competitive assay. Western blot technique was adopted to identify the mAb. The proliferation of tumor cells in vitro was analyzed by MTT assay and apoptosis of tumor cells in vitro was analyzed by PI-annexin V assay. RESULTS: One hybridoma cell line named 10C5 was obtained, which had the property of secreting anti-human CD40 mutant monoclonal antibody continuously and steadily. This mAb specifically recognized human CD40 mutant molecule and induced the apoptosis of tumor cells in vitro. CONCLUSION: One hybridoma cell line which can secret a mouse anti-human CD40 mutant mAb has been prepared successfully. This mAb can inhibit the growth of tumor cells expressing CD40 mutant and induce their apoptosis in vitro.%目的:以本科室发现肿瘤细胞上表达的CD40 787AA突变为基础,研制识别肿瘤细胞上CD40突变体分子的单克隆抗体(mAb),并对其生物学特性作初步分析.方法:以转人CD40突变体转基因细胞L929-CD40mu为免疫原,免疫6~8周龄的雌性BALB/c小鼠;采用B淋巴细胞融合技术,将免疫小鼠脾脏细胞与Sp2/0融合,以L929-CD40mu转基因细胞为抗体筛选阳性细胞,免疫荧光标记法对杂交瘤进行反复筛选和多次的克隆化培养;采用快速定性试纸法及竞争抑制结合试验分析该mAb的亚类及抗原识别位点;免疫印迹法对该mAb进行鉴定;采用MTT法分析mAb在体外对肿瘤细胞的抑制增殖效应以及PI-annexin V方法进行细胞凋亡测定.结果:获得1株稳定分泌鼠抗人CD40mu m

  18. The Observation Effects of Immune Globulin and Conventional Therapy in Guillain-Barre Syndrome%免疫球蛋白和常规方法治疗格林巴利综合征患者的疗效对比观察

    Institute of Scientific and Technical Information of China (English)

    余鸽鸽

    2015-01-01

    目的:对格林巴利综合征治疗中常规方法与免疫球蛋白的使用效果进行对比观察。方法将本院于2013年6月~2015年6月收治的格林巴利综合征患者74例纳为研究对象,随机分成对照组与治疗组,分别采取常规综合治疗与免疫球蛋白治疗。进行两组生活活动能力、起效时间、总有效率的对比观察。结果治疗组的总有效率(94.59%)高于对照组(78.38%),治疗组的起效时间较快,且治疗后生活活动能力较好,组间对比,P<0.05,差异有统计学意义。结论格林巴利综合征治疗中免疫球蛋白的使用效果突出。%Objective To compare the effect of immune globulin and conventional therapy for Guillain-Barre syndrome patients.Methods The hospital in June 2013 to June 2015, 74 cases of Guillain-Barre syndrome were satisfied for the study, were randomly divided into control and treatment groups, were taken to routine treatment with immune globulin therapy. Two sets of living activity, onset time, total contrast observation efficiency.Results The total efficiency of the treatment group (94.59%) higher (78.38%), onset time faster treatment group, and the ability to better treatment after living activities, group comparison,P<0.05, the difference was statistically signiifcant. Conclusion Guillain-Barre syndrome treatment effect immune globulin prominent.

  19. 低分子肝素联合静滴丙种球蛋白治疗复发性流产临床疗效分析%Clinical Efficacy of Low Molecular Heparin Combined with Intravenous Gamma Globulin in the Treatment of Recurrent Miscarriage

    Institute of Scientific and Technical Information of China (English)

    邓金枝

    2014-01-01

    目的:观察分析低分子肝素联合静滴丙种球蛋白治疗复发性流产的临床疗效。方法:选取2011年6月-2012年6月笔者所在医院收治的复发性流产患者60例,随机分为观察组和对照组,每组30例,观察组采用低分子肝素联合静滴丙种球蛋白治疗,对照组采用传统保胎法,观察两组的保胎成功率和不良反应发生情况。结果:观察组保胎成功率为86.67%,显著高于对照组的33.33%,差异有统计学意义(P0.05)。结论:低分子肝素联合静滴丙种球蛋白治疗复发性流产,保胎效果好,安全性高,值得临床推广。%Objective:To observe and analyze the clinical efficacy of Low Molecular Heparin combined with intravenous Gamma Globulin in the treatment of recurrent miscarriage.Method:60 cases with recurrent miscarriage were selected,who received treatment in our hospital from June 2011 to 2012 June.They were divided into observation group and control group,30 cases in each group.Low Molecular Heparin combined with intravenous Gamma Globulin was taken by observation group.The traditional method of miscarriage was taken by the control group.The success rate of miscarriage and adverse reactions were observed. Result:Miscarriage success rate of observation group was 86.67%,which was significantly higher than the control group 33.33%,the difference had a statistical significance(P0.05).Conclusion:Low Molecular Heparin combined with intravenous Gamma Globulin has a effect for miscarriage in the treatment of recurrent abortion,with a high safety,which is worthy of promotion.

  20. 静脉滴注丙种球蛋白联合低分子肝素联合治疗42例复发性流产临床疗效观察%Intravenous infusion of gamma globulin combined with low molecular heparin in treatment of recurrent miscarriage

    Institute of Scientific and Technical Information of China (English)

    桑艳萍; 徐静; 李艳红

    2013-01-01

    Objectives:To investigate clinical efficacy and safety of intravenous infusion of gamma globulin and LMWH in treating recurrent miscarriage.Methods:84 recurrent miscarriage patients of anti-cardiolipin antibodies (ACA)-positive were randomly divided into experimental group and control group.Patients in the experimental group were treated with intravenous infu sion of gamma globulin combing low-molecular-weight heparin while the control group was given the traditional miscarriage treatment such as progesterone combined with HCG.Tocolytic.The success rate and the incidence of adverse effect in the two groups were compared.Results:Tocolysis rate of the experimental group was 81.0%.the control group 31.0%,which was much lower (p < 0.05).Patients in the two groups had no serious adverse reactions.Conclusions:Intravenous infusion of gamma globulin and LMWH treatment can be used among ACA positive patients with recurrent miscarriage after overall considering its efficacy and safety.%目的:探讨静脉滴注丙种球蛋白联合低分子肝素对于复发性流产孕妇的临床疗效及安全性.方法:将本院收治的84例抗心磷脂抗体(ACA)阳性的复发性流产患者随机分为实验组和对照组,实验组患者给予静脉滴注丙种球蛋白联合低分子肝素治疗,对熙组患者给予黄体酮联合HCG的传统保胎治疗,比较两组患者保胎成功率及不良反应的发生情况.结果:实验组保胎成功率81.0%,对照组保胎成功率31.0%,实验组保胎成功率显著高于对照组(P<0.05).两组患者均无严重不良反应发生.结论:对于ACA阳性的复发性流产患者,静脉滴注丙种球蛋白联合低分子肝素疗效显著且安全性高,值得临床推广应用.

  1. Comparison of low dose and high dose human gamma globulin in therapy for acute Guillain-Barre syndrome in infants%不同剂量丙种球蛋白治疗儿童吉兰-巴雷综合征的疗效比较

    Institute of Scientific and Technical Information of China (English)

    唐军

    2013-01-01

    目的 观察不同剂量丙种球蛋白(IVIG)治疗儿童吉兰-巴雷综合征(GBS)的临床疗效.方法 将符合GBS诊断的141例患者分为三组,分别给予不同剂量IVIG,并对疗效进行对比观察.结果 三种给药方式对轻、中、重度患者的疗效差异无统计学意义(均P> 0.05),不同程度患者总有效率分别为95.83%、95.74%、82.85%.结论 IVIG治疗儿童吉兰-巴雷综合征具有良好的效果,低剂量组和大剂量组差异无统计学意义,低剂量IVIG治疗儿童GBS亦有效.%Objective To observe the therapy effect of different dosages human gamma globulin (IVIG) for acute Guillain-Barre syndrome.Methods 134 cases of acute Guillain-Barre syndrome were divided into three groups.They were given different dosages of IVIG.Clinical effects were compared and observed.Results The curative effect of three kinds of drug ways for light, medium and severe patients had no significant differences (all P > 0.05).The total effective rate of patients with different level was 95.83%, 95.74%, 82.85% respectively.Conclusion IVIG in the treatment of children with Guillain-Barre syndrome has a good effect.There is no significant difference between low dose and high dose human gamma globulin for acute Guillain-Barre syndrome.Low dosage IVIG in treating children with Guillain-Barre syndrome is also effective.

  2. Effect of fluoride exposure on sex hormone binding globulin and estradiol in adult female%氟暴露对成年女性性激素结合蛋白及雌二醇水平的影响

    Institute of Scientific and Technical Information of China (English)

    赵明旭; 李世宏; 崔蕊蕊; 任永成; 丁中; 后加祥; 周桐; 程学敏; 崔留欣

    2014-01-01

    目的 探讨饮水氟暴露对成年女性性激素结合蛋白(SHBG)及雌二醇水平的影响.方法 采用现况调查,依据水氟检测结果,在河南省通许县抽取7个村作为调查点,其中2个为高氟村(高氟组)、2个为实施降氟改水工程村(改水组)和3个对照村(对照组);整群抽取调查点本地生长或居住5年以上的18~48岁成年女性,采集饮用水、晨尿和空腹静脉血.应用氟离子选择电极法检测饮用水和尿中氟含量;酶联免疫吸附法(ELISA)检测血清SHBG含量;化学发光免疫分析法检测血清雌二醇含量.结果 高氟组、改水组和对照组水氟含量分别为2.44、0.36、0.37 mg/L,组间比较差异有统计学意义(F=12.289,P<0.01),其中高氟组分别高于改水组与对照组(P均< 0.05).高氟组、改水组和对照组尿氟含量分别为2.58、145、0.96 mg/L,组间比较差异有统计学意义(F=130.418,P<0.05),其中高氟组分别高于改水组与对照组(P均<0.01),改水组高于对照组(P<0.01).高氟组、改水组和对照组血清SHBG浓度分别为41.63、50.68、57.19 nmol/L,组间比较差异有统计学意义(F=3.806,P<0.05),其中高氟组低于对照组(P<0.01).高氟组、改水组和对照组血清雌二醇浓度分别为54.29、75.57、75.00 nmol/L,组间比较差异有统计学意义(F=5.481,P< 0.05),其中高氟组分别低于改水组与对照组(P均< 0.01).高氟组血清雌二醇含量与年龄呈负相关(r=-0.224,P<0.01).结论 长期氟暴露能够影响成年女性血清SHBG和雌二醇的水平.%Objective To explore the influence of water fluoride exposure on sex hormone binding globulin(SHBG) and estradiol(E2) in adult female Methods Cross-sectional study was conducted in seven villages of Tongxu county including two high fluoride groups (HFG),two defluoridation project groups(DFPG) and three control groups (CG) based on the fluoride concentration in drinking water.Adult female who was born and groun in the

  3. Preparation and preliminary application of anti-human breast cancer MUC1 monoclonal antibody%抗乳腺癌多形上皮黏蛋白1单克隆抗体的制备及初步应用

    Institute of Scientific and Technical Information of China (English)

    李航; 袁时芳; 凌瑞; 贠军; 李永平; 王辉; 李郁; 宋朝君; 刘佩娟

    2015-01-01

    目的 制备并鉴定抗多形上皮黏蛋白1(MUC1)单克隆抗体,将抗体应用于人乳腺癌组织、纤维腺瘤、囊性增生症及正常乳腺组织的临床鉴别,采用免疫组化方法进行检测.方法 用合成的MUC1重复序列短肽与钥孔血蓝蛋白(KLH)及牛血清白蛋白(BSA)偶联后作为抗原免疫Balb/c小鼠,杂交瘤技术筛选出稳定分泌单克隆抗体的细胞株.制备单克隆抗体,以ELISA、Western blot、流式细胞术、免疫荧光法对抗体进行特异性鉴定及亚类分析;将抗体应用于人乳腺癌组织、纤维腺瘤、囊性增生症及正常乳腺组织的临床鉴别,采用免疫组化方法进行检测.结果 获得4株稳定分泌抗MUC1的杂交瘤细胞株,第1株反应性最强,效价1∶10 7以上.抗体亚型3株为IgG1,1株为IgM,轻链均为κ型.经Western blot、流式细胞术、免疫荧光、免疫组化检测证实抗体与乳腺癌细胞株MCF-7特异性结合,单抗检测MUC1在乳腺癌组织与纤维腺瘤、囊性增生症及正常乳腺组织之间表达差异明显(均P<0.01).结论 抗MUC1单克隆抗体经检测效价高、特异性强,为乳腺癌早期诊断和靶向治疗的进一步研究奠定了基础.%Objective To prepare the anti-human MUC1 monoclonal antibody (mAb) and to apply it in human breast cancer tissues,fibro adenoma,cystic hyperplasia and normal breast tissues,using immunohistochemistry assay.Methods Synthetic MUC1 repetitive sequence short peptide coupled with KLH protein and BSA protein as antigen to immunize Balb/c mice,cell lines capable of secreting monoclonal antibodies were screened by hybridoma cell fusion technique.The specificities and subclasses of the prepared monoclonal antibodies were identified and analyzed by ELISA,Western blot,flow cytometry and immunofluorescence test.The antibodies were used in the detection of clinical human breast cancer,benign fibro adenoma and normal breast tissue by immunohistochemical methods.Results Four

  4. Característica leucocitária, relação albumina/globulina, proteína plasmática e fibrinogênio de bovinos da raça Nelore, confinados e terminados a pasto Leukocyte characteristic, albumin/globulin relation, plasmatic protein and fibrinogen of bovines of the Nelore race confined and grazing

    Directory of Open Access Journals (Sweden)

    Ediane Batista da Silva

    2008-11-01

    .This research aimed to evaluate the changes in the white blood cell count and some serum proteins of confined cattle (CC and grass cattle (GC. From the 120 blood samples collected, 60 were obtained from confined Nelore male bovines and 60 from animals with the same characteristics but managed extensively. Samples were obtained at the moment of slaughter. Parameters studied were the white blood cell count, serum albumin/globulin ratio and concentration of plasma fibrinogen. Descriptive statistics was used in the analysis of the data, and the averages, standard deviation and coefficient of variation calculated for all parameters evaluated. The comparisons between averages were made by non-parametric test. The grazing cattle showed higher levels of globulin and fibrinogen when compared to the confined ones (globulin: GC=3.29g dL-1±0.76; CC=2.99g dL-1±0.60 and Fibrinogen: GC=872mg dL-1±610; CC=633mg dL-1±319. The total number of white blood cells mL-1 was 7.64±2.15 in confined cattle and 7.72±1.84 in grazing cattle. There was no significant difference between this variable and differential white blood cell count as well as the total serum protein (g dL-1 from grazing cattle (6.10±0.53 and confined cattle (5.96±0.49. The level of albumin from confined cattle (3.01g dL-1± 0.43 and the A/G ratio (1.07±8.91 were greater when compared to the grazing bovines (2.82g dL-1±0.45 and (0.95±0.38 respectively. The higher serum levels of albumin found in confined herd suggest that they were subjected to a more adequate nutritional diet. The constant immunological challenge suffered by the GC could be responsible for the elevated serum levels of globulin and fibrinogen. These results showed that although feedlots present a stressful environment they did not show any blood alterations correlated to this fact.

  5. Alterations of serum concentrations of thyroid hormones and sex hormone-binding globulin, nuclear binding of tri-iodothyronine and thyroid hormone-stimulated cellular uptake of oxygen and glucose in mononuclear blood cells from patients with non-thyroidal illness

    DEFF Research Database (Denmark)

    Kvetny, J; Matzen, L

    1990-01-01

    with healthy control subjects (1.45 +/- 0.30 nmol/l). Neither serum TSH nor sex hormone-binding globulin differed from that of the control group. Nuclear T3 binding capacity was increased (P less than 0.05) in patients with NTI (10.1 +/- 3.0 fmol/100 micrograms DNA) compared with controls (2.5 +/- 0.9 fmol/100......Nuclear tri-iodothyronine (T3) binding and thyroid hormone-stimulated oxygen consumption and glucose uptake were examined in mononuclear blood cells from patients with non-thyroidal illness (NTI) in which serum T3 was significantly (P less than 0.05) depressed (0.62 +/- 0.12 (S.D.) nmol/l) compared.......05) compared with controls (0.24 +/- 0.10 mmol/l per mg DNA per h). In contrast, neither unstimulated nor thyroid hormone-stimulated oxygen consumption differed. We conclude that both increased nuclear T3 binding and increased thyroid hormone-induced glucose uptake may represent counter-regulatory mechanisms...

  6. 兔抗人胸腺细胞免疫球蛋白治疗重型再生障碍性贫血25例疗效分析%The therapeutic effects of Antithymocyte globulin to treat children with severe aplastic anemia

    Institute of Scientific and Technical Information of China (English)

    李玉玲; 薛惠良

    2011-01-01

    Objective To evaluate the effects and side effects of Antithymocyte globulin (ATG)to treat children with severe aplastic anemia (SAA). Methods The therapeutic effects of 25 children with SAA treated by Antithymocyte globulin were analyzed retrospectively from 2004 to 2009. Peripheral hemogram, long-term clone change and infection were observed after treatment for more than 6 months.Results ATG were effective in 17 children (68%). Among them, five (20%) cases were nearly cured, three (12%) got remission and nine (36%) got improved. Eight (32%) cases had no response to ATG treatment. Among them, two (8%) cases were cured after receiving allogeneic hematopoietic stem cell transplantation and two (8%) died because of infection after 6 months. One (4%) case had a clone change into MDS. One case (4%) got rash, fever and joint pain and two cases (8%) got laryngeal edema during ATG infusion. Some children had serum reaction in two weeks after ATG infusion. Conclusion The effective rate of ATG was 68%. The side effects of ATG included rash, fever and joint pain. Laryngeal edema was rare. Corticosteroid hormone should be considered to prevent allergic and serum reactions during ATG treatment.%目的 探讨使用兔抗人胸腺细胞免疫球蛋白(ATG)治疗重型再生障碍性贫血(SAA)的疗效及毒副反应.方法 回顾性分析2004-2009年共25例SAA患儿应用ATG+环孢素A+雄激素治疗后随访半年以上的疗效.观察患儿血象变化、远期克隆性改变、感染.结果 ATG治疗半年后25例患儿中17例(68%)有效,其中5例(20%)基本治愈,3例(12%)缓解,9例(36%)明显进步,8例(32%)无效中2例(8%)行异基因造血干细胞移植后治愈,2例(8%)半年后因感染死亡.随访期间1例SAA-II型发生远期克隆性改变转变为MDS.1例患儿在输注ATG过程中出现皮疹、发烧、关节痛;2例出现喉头水肿;输注后2周内患儿出现血清病反应,表现为皮疹、发热、关节痛.第1周7例,第2

  7. Single-nucleotide polymorphism, rs1799941 in the Sex Hormone-Binding Globulin (SHBG) gene, related to both serum testosterone and SHBG levels and the risk of myocardial infarction, type 2 diabetes, cancer and mortality in men: the Tromsø Study.

    Science.gov (United States)

    Svartberg, J; Schirmer, H; Wilsgaard, T; Mathiesen, E B; Njølstad, I; Løchen, M-L; Jorde, R

    2014-03-01

    Low testosterone levels are associated with metabolic and cardiovascular disease risk factor, and have been shown to predict type 2 diabetes mellitus (T2DM), myocardial infarction (MI) and all-cause mortality. It is not known if these associations are causal or not. Recently, it has been shown that the serum testosterone levels are associated with single-nucleotide polymorphisms (SNPs), and we therefore studied the associations between one of these SNPs, rs1799941 on the Sex Hormone-Binding Globulin (SHBG) gene, and MI, T2DM, cancer and death. DNA was prepared from men who participated in the fourth survey of the Tromsø Study in 1994-1995 and who were registered with the endpoints MI, T2DM, cancer or death and a randomly selected control group. For mortality, the observation time was set from 1994, and for the other endpoints from birth. The endpoint data were completed up to 2010-2013. Genetic analyses were successfully performed in 5309 men, of whom 1454 were registered with MI, 638 with T2DM, 1534 with cancer and in 2226 who had died. Men with the minor homozygote genotype had significantly higher levels of total testosterone (14.7%) and SHBG (24.7%) compared with men with the major homozygote genotype, whereas free testosterone levels did not differ significantly between the genotypes. The SNP rs1799941 was not significantly associated with MI, T2DM, cancer or mortality. Thus, our result does not support a causal relationship between total testosterone and SHBG and MI, T2DM, cancer or mortality, suggesting that low testosterone more likely is a marker of poor health.

  8. Researches on the association between sex hormone binding globulin levels in the first trimester of pregnancy and subsequent gestational diabetes mellitus%早孕期血清SHBG水平与妊娠期糖尿病发病的关系

    Institute of Scientific and Technical Information of China (English)

    金镇; 王娉婷; 王晓岩; 许奇伟; 艾婉婷

    2010-01-01

    目的:探讨早孕期血清性激素结合球蛋白(sex hormone binding globulin,SHBG)水平与妊娠期糖尿病(gestational diabetes mellitus,GDM)发病关系.方法:采用ELISA法分别测定GDM发病期和早孕(妊娠10+1~12周)期的孕妇血清SHBG水平并进行分析.结果:早孕期血清SHBG水平与GDM发病时间Spearman等级相关,r=0.9739.Logistic多元回归分析结果表明早孕期血清SHBG水平作为连续性变量每升高50 nmol/L,GDM危险下降53%(OR及95%CI:0.474,0.306~0.736);以中位数(Median)将早孕期血清SHBG水平分为二分类变量,结果表明SHBG≤407.8 nmol/L相对于SHBG>407.8 nmol/L发展成GDM危险增加(OR及95%CI:3.615,2.195~5.956).结论:早孕期血清SHBG水平降低明显预示着将来GDM发病的可能;早孕期血清SHBG水平降低程度不但可以作为早期预测GDM发病一个指标,而且还可以判定GDM发病的时间和程度.

  9. 支气管镜肺段灌洗联合丙种球蛋白治疗多药耐药菌肺炎的疗效研究%Study on the efficacy of lung lavage by bronchoscope combined with gamma globulin in the treatment of multi-drug resistant pneumonia

    Institute of Scientific and Technical Information of China (English)

    陈云辉; 郑利先; 罗巍; 曾博文; 陈自瑜

    2015-01-01

    OBJECTIVE To observe the efficacy of lung lavage by bronchoscope combined with gamma globulin in the treatment of multi‐drug resistant pneumonia so as to improve the curative rate of pneumonia .METHODS Total‐ly 78 cases of patients with multidrug‐resistant pneumoniae caused by K lebsiella were chosen as research objects , and all the patients were randomly divided into two groups:41 cases in the experiment group and 37 cases in the control group .Skin test was conducted to the control group to choose the suitable antibiotics for routine therapy . On the basis of this ,the observation group used lung lavage by bronchoscope combined with gamma globulin .The related indexes of Qi and blood analysis and curative effect were observed in the two groups before and after the treatments .RESULTS The effectual rate in the experimental group was 43 .9% ,significantly higher than the con‐trol group of 13 .5% .And the total effective rate in the experiment group was 90 .2% ,also significently higher than the control group of 70 .3% (P<0 .05) .After the treatments ,significant differences existed in arterial par‐tial pressure of oxygen ,carbon dioxide and arterial oxygen saturation between the two groups (P<0 .05) .No sig‐nificant differences were found between the two groups by lung functions .But after treatments ,significant differ‐ences existed in indexes like forced vital capacity ,forced expiratory volume in one second and respiratory rate in a second between the two groups (P<0 .05) .CONCLUSION Lung lavage by bronchoscope combined with gamma globulin is an effective method for curing severe pulmonary diseases with remarkable clinical efficacy and safety , hence is of high clinical value in treating multi‐drug resistant pneumonia .%目的:观察经支气管镜肺段灌洗联合丙种球蛋白治疗多药耐药菌肺炎的临床疗效,以提高肺炎的治愈率。方法选择78例多药耐药克雷伯菌属所致的肺炎患者作为研究对象,

  10. Clinical Studies of Mother to Child Transmission of Hepatitis B Virus in Late Pregnancy Injection of Hepatitis B Immuno-globulin Blocking%妊娠晚期注射乙肝免疫球蛋白阻断乙肝病毒母婴传播的临床研究

    Institute of Scientific and Technical Information of China (English)

    高争; 谭乐静; 王晓红

    2014-01-01

    B virus .Methods :Between January 2011 to January 2013 admitted for treatment of hepatitis B surface antigen ,hepatitis Be antigen-positive pregnant women were 160 cases ,according to figures randomly were randomly divided into experimental group (80 cases) and control group (80 cases) .Each experimental group at 28 weeks of pregnancy intervals beginning four weeks intramuscularly once a hepatitis B immune globulin (200 IU) ,a total of three injections ;late pregnancy does not control injection of hepatitis B immune globulin .After the fetus phone cord blood of hepatitis B surface antigen determination to observe the effects of injection of hepatitis B immune globulin in late pregnancy on infant immunization effect .Results :Neonatal infection ,the experimental group were negative for HB-sAg and HBeAb 69 cases ,accounting for 86 .25% in the control group 57 cases ,accounting for 71 .25% ;10 cases of HBsAg positive only in the experimental group ,accounting for 12 .50% ,control group 18 cases ,accounting for 22.50% ;HBsAg were positive with HBeAb experimental group 1 case ,accounting for 1 .25% of the control group ,5 cases (6 .25% );experimental intrauterine infection rate was 12 .50% in the control group was 27 .50% ;difference was significant (P0 .05);26 cases of low-risk group of pregnant women in the experimental group ,neonatal infection rate 15 .38% ,the low-risk group of pregnant women in the control group ,23 cases of neonatal infection rate was 21 .74% ,there was no significant difference (P> 0 .05);experimental group of high-risk pregnant women 32 cases of group ,neonatal infection rate was 15 .63% ,the high-risk group of preg-nant women in the control group ,34 cases of neonatal infection rate was 44 .12% ,the difference was significant (P 0.05) .Conclusion:Late pregnancy intramuscular HBIG can effectively reduce intrauterine infection ;pregnancy HBV-DNA for low risk or low-risk pregnant women in late pregnancy HBIG injection for blocking M TCT no

  11. Impact of Thymosin on Children with Recurrent Respiratory Tract Infections Clinical Efficacy and Immune Globulin%胸腺肽对小儿反复呼吸道感染临床疗效及免疫球蛋白的影响

    Institute of Scientific and Technical Information of China (English)

    苗永红

    2014-01-01

    目的:临床应用胸腺肽观察对小儿反复呼吸道感染疗效及免疫球蛋白的影响。方法选择我院2008年1月~2012年2月小儿反复呼吸道感染住院病例72例,随机分为治疗组40例,对照组32例。治疗组在一般的抗炎及对症处理的基础上加用胸腺肽,对照组给予常规抗感染及对症治疗,分别用χ2检验和t检验的统计学方法比较两组患儿的临床疗效及治疗前后的血浆IgG、IgA、IgM的水平。结果治疗组疗效优于对照组(P<0.01),治疗组治疗后IgG、IgA、IgM较治疗前差异有显著性(P<0.01)。结论临床应用胸腺肽能调节小儿体液免疫免疫水平,减少小儿反复呼吸道感染的发病率,提高疗效。%Objective:Clinical use thymus peptide observations on the child repeatedly respiratory infections and curative ef ect of immunity globulins occurring. Methods:Select the child repeatedly respiratory tract infection72cases randomly divided into groups of 40 for example,in the 32 cases.the treatment of the fight and to the at ack on the basis of processing and use of the thymus peptide and give resistance to infection of the convention and the treatment of intervertebral. Results:Of the curative treatment beats (p< 0.01)and treatment groups treatment igg and igm iga,a significant dif erence in treatment before a sexual (p<0.01). Conclusion:Clinical peptide thymus gland reduces the incidence of respiratory tract infection of the child repeatedly.child immunization.

  12. Observation on the effect of different doses of gamma globulin in the treatment of children with severe HFMD%不同剂量丙种球蛋白治疗儿童重型手足口病疗效观察

    Institute of Scientific and Technical Information of China (English)

    廖先辉

    2016-01-01

    目的:观察不同剂量丙种球蛋白治疗重型小儿口足病的临床疗效。方法遵义市妇幼保健院儿科2013年6月至2015年6月收治2期、3期手足口病患儿96例,随机分为小剂量组和大剂量组各48例。两组患儿在常规治疗和对症治疗的基础上静脉注射不同剂量丙种球蛋白,小剂量组注射剂量为1 g/kg,大剂量组注射剂量为2 g/kg,比较两组患儿临床症状消失时间、临床治愈时间、住院时间、并发症以及经济效益。结果两组患儿临床症状消失时间、临床治愈时间、住院时间比较差异无统计学意义(P >0.05);大剂量组患儿的并发症发生率为16.7%(8/48),高于对照组2.1%(1/48),差异有统计学意义(P 0.05).The complication incidence was 1 6.7%(8/48)in the large-dose group,higher than the small-dose one,which was 2.1%(1/48),and the difference was statistical significance(P <0.05). The expense in the small-dose group was lower.Conclusion The effect of gamma globulin at different doses is similar and significant in the treatment of severe HFMD.When the dose is 1 g/kg,the treatment expense is lower and the complication incidence is lower,so it is worthy of being clinically applied.

  13. Effect Observation of Reduning Injection Combined with Intravenous Gamma Globulin in the Treatment of Hand Foot and Mouth Disease%热毒宁注射液联合静脉丙种球蛋白治疗手足口病的效果观察

    Institute of Scientific and Technical Information of China (English)

    金芳

    2015-01-01

    Objective: To investigate the effect of Reduning combined with intravenous gamma globulin (IVIG) in children with the clinical effect of treatment of severe hand foot mouth disease.Methods:Using random number table method,200 cases of children with hand foot in this hospital from 2010 August to 2012 July were mouth disease were divided into study group and control group with 100 cases in each group,the control group was given conventional treatment,+IVIG treatment,study group based on the use of Reduning,observe the clinical effect and changes of 7D after treatment between two groups of children.Results:The study group regression fever (3.4±1.2) (d),rash (2.5±0.8) (d),oral ulcers subside (2.6±0.9) (d),nervous system symptoms subsided (3.1±1.5) (d) were significantly lower than that of the control group (P < 0.05). The study group and the control group before treatment in children with inflammatory factor TNF- alpha,CRP,IL-6 and myocardial enzymes CK-MB,CK values were not significantly different,after treatment,the indexes of two groups before treatment were significantly decreased (P < 0.05),the study group compared with the control group decreased significantly (P < 0.05). After the treatment of 7D in the study group in the total efficiency is 98% higher than that of the control group and the difference was significant (P < 0.05 91%).Conclusion:Children with Reduning combined with intravenous gamma globulin in the treatment of severe hand foot mouth disease can significantly improve the therapeutic effect.%目的:探讨热毒宁联合静脉丙种球蛋白(IVIG)治疗重症手足口病患儿的临床效果.方法:采用随机数字表法将本院儿科2010年8月至2012年7月收治的200例手足口病患儿分为研究组和对照组各100例,对照组予以常规治疗+IVIG治疗,研究组在此基础上加用热毒宁,观察两组患儿治疗7d后的临床疗效及指标变化差异.结果:研究组的发热消退(3.4±1.2)(d)、皮疹消退(2.5± 0.8)(d

  14. 抗胸腺细胞免疫球蛋白治疗儿童再生障碍性贫血的不良反应相关因素及疗效相关性研究%Adverse reactions management of Antithymocyte Globulin in treatment of childhood with aplastic anemia

    Institute of Scientific and Technical Information of China (English)

    李福兴; 石苇; 乔晓红; 周晓迅; 何薇; 谢晓恬

    2016-01-01

    目的:归纳抗胸腺细胞免疫球蛋白(ATG)免疫抑制治疗儿童再生障碍性贫血(AA)不良反应防治对策,评价不良反应与疗效的关系,分析不良反应发生的相关因素。方法采用ATG联合环孢菌素A(CsA)联合免疫抑制疗法(CIS)治疗儿童再生障碍性贫血共61例,其中使用美国(Genzyme公司)R- ATG 31例,使用德国(Fresenius公司)R- ATG 30例。采取不良反应综合防治措施,比较两种ATG不良反应发生率,分析不良反应与疗效关系,分析不良反应的相关因素。结果 Fresenius- ATG相关类过敏反应、血清病、感染和需加输血小板等不良反应发生率分别为40.00%(12例)、43.30%(13例)、16.70%(5例)和23.33%(7例);Genzyme- ATG相关类过敏反应、血清病、感染和需加输血小板等不良反应发生率分别为35.48%(11例)、35.48%(11例)、9.70%(3例)和29.03%(9例),两种制剂ATG不良反应发生率差异无统计学意义(>0.05)。不良反应的发生与否疗效比较差异无统计学意义,不良反应发生率在不同年龄,性别和疾病分型中差异无统计学意义。经综合防治,所有不良反应均得以及时控制,无治疗相关死亡。结论 ATG联合CsA治疗儿童再生障碍性贫血安全有效,综合防治措施可有效防治不良反应。Fresenius- ATG和Genzyme- ATG治疗儿童再生障碍性贫血不良反应发生率相当;不良反应的发生并不影响ATG治疗效果;不同年龄、性别及疾病严重程度与不良反应发生无直接相关性。%Objective To summarize the adverse reactions of rabbit antithymocyte globulin in treatment of chil-dren with aplastic anemia. Methods 61 children with AA treated with ATG and CsA in our department were ana-lyzed, 31 of them received (Genzyme) R-ATG and CsA, the other 30 received Fresenius-ATG and CsA therapy, comprehensive measures were used to prevent and control ATG

  15. Influence of water fluoride exposure on sex hormone binding globulin and testosterone in adult male%饮水氟暴露对成年男性性激素结合球蛋白及睾酮水平的影响

    Institute of Scientific and Technical Information of China (English)

    周桐; 杨如璞; 李世宏; 郑国庆; 席豫; 程学敏; 后加祥; 崔留欣; 巴月

    2013-01-01

    Objective To explore the influence of water fluoride exposure on sex hormone binding globulin ( SHBG) and testosterone in adult male. Methods Cross-sectional study was conducted in three villages of Tongxu county including high fluoride group (HFG) , defluoridation project group (DFPG) and control group (CG) based on the fluoride concentration in drinking water. Adult male who were born and raised in the village and aged 18-50 years old were recruited using cluster sampling. Fasting blood and morning urine samples were collected. The fluoride levels in drinking water and urine were detected by fluoride-ion selective electrode method. Serum SHBG level was determined using enzyme-linked immunosorbent assay ( ELISA ). The chemical luminescence immune analysis method was used to detect serum testosterone content. Results Serum SHBG level was 47. 85nmol/L in CG, 31.37nmol/L in DFPG and 24. 52nmol/L in HFG respectively. There were significant difference among of three groups (P <0. 05). Serum testosterone level was 3. 69ng/ml in CG, 4. 6Ing/ml in DFPG and 4. 83ng/ml in HFG respectively. Serum testosterone level in HFG was significantly higher than that in CG ( P < 0. 05 ). Serum SHBG level in HFG has positive correlation with serum testosterone ( r = 0. 230 , P = 0. 049 ) , which has not been observed in DFPG and CG. Conclusions Long-time fluorine exposure may affect serum SHBG and testosterone level in adult male.%目的 探讨饮水氟暴露对成年男性性激素结合球蛋白(SHBG)及睾酮水平的影响.方法 依据水氟检测结果,在河南省通许县随机选择3个村庄作为调查点,分别为高氟村、改水村和对照村;整群抽取调查区本地生长的18 ~ 50岁成年男性,收集空腹静脉血和晨尿.应用氟离子选择电极法测定饮用水和尿中氟的含量;用酶联免疫吸附法(ELISA)测定其血清中SHBG水平,化学发光法测定血清睾酮的含量.结果 血清SHBG浓度分别为对照组47.85nmol/L,改水组31

  16. Induction of Murine Mucosal CCR5-Reactive Antibodies as an Anti-Human Immunodeficiency Virus Strategy

    Science.gov (United States)

    Barassi, C.; Soprana, E.; Pastori, C.; Longhi, R.; Buratti, E.; Lillo, F.; Marenzi, C.; Lazzarin, A.; Siccardi, A. G.; Lopalco, L.

    2005-01-01

    The genital mucosa is the main site of initial human immunodeficiency virus type 1 (HIV-1) contact with its host. In spite of repeated sexual exposure, some individuals remain seronegative, and a small fraction of them produce immunoglobulin G (IgG) and IgA autoantibodies directed against CCR5, which is probably the cause of the CCR5-minus phenotype observed in the peripheral blood mononuclear cells of these subjects. These antibodies recognize the 89-to-102 extracellular loop of CCR5 in its native conformation. The aim of this study was to induce infection-preventing mucosal anti-CCR5 autoantibodies in individuals at high risk of HIV infection. Thus, we generated chimeric immunogens containing the relevant CCR5 peptide in the context of the capsid protein of Flock House virus, a presentation system in which it is possible to engineer conformationally constrained peptide in a highly immunogenic form. Administered in mice via the systemic or mucosal route, the immunogens elicited anti-CCR5 IgG and IgA (in sera and vaginal fluids). Analogous to exposed seronegative individuals, mice producing anti-CCR5 autoantibodies express significantly reduced levels of CCR5 on the surfaces of CD4+ cells from peripheral blood and vaginal washes. In vitro studies have shown that murine IgG and IgA (i) specifically bind human and mouse CD4+ lymphocytes and the CCR5-transfected U87 cell line, (ii) down-regulate CCR5 expression of CD4+ cells from both humans and untreated mice, (iii) inhibit Mip-1β chemotaxis of CD4+ CCR5+ lymphocytes, and (iv) neutralize HIV R5 strains. These data suggest that immune strategies aimed at generating anti-CCR5 antibodies at the level of the genital mucosa might be feasible and represent a strategy to induce mucosal HIV-protective immunity. PMID:15890924

  17. Reactivity of eleven anti-human leucocyte monoclonal antibodies with lymphocytes from several domestic animals

    DEFF Research Database (Denmark)

    Aasted, Bent; Blixenkrone-Møller, Merete; Larsen, Else Bang;

    1988-01-01

    Nine commercially available monoclonal antibodies and two monoclonal antibodies from The American Type Culture Collection, raised against various human leucocyte surface antigens, were tested on lymphocytes from cow, sheep, goat, swine, horse, cat, dog, mink, and rabbit as well as man. Four...

  18. Anti-Human T-Lymphotropic Virus Type-I Antibodies in Atomic-Bomb Survivors

    OpenAIRE

    Matsuo, Tatsuki; Nakashima, Eiji; Carter, Randolph L.; Neriishi, Kazuo; Mabuchi, Kiyohiko; Akiyama, Mitoshi; Shimaoka, Katsutaro; Kinoshita, Ken-Ichiro; Tomonaga, Masao; Ichimaru, Michito

    1995-01-01

    Adult T-cell leukemia (ATL), induced by human T-lymphotropic virus type-I (HTLV-I), is endemic in Nagasaki, Japan. To investigate the effects of atomic-bomb radiation on development of this specific type of leukemia, 6182 individuals in the Radiation Effects Research Foundation (RERE) Adult Health Study sample in Hiroshima and Nagasaki were examined for positive rate of HTLV-I antibody. Several lymphocyte parameters were also studied for 70 antibody-positive subjects in Nagasaki. The HTLV-I a...

  19. Production of the Polyclonal Anti-human Metallothionein 2A Antibody with Recombinant Protein Technology

    Institute of Scientific and Technical Information of China (English)

    Faiz M.M.T.MARIKAR; Qi-Ming SUN; Zi-Chun HUA

    2006-01-01

    Metallothionein 2A (MT2A) is a small stress response protein that can be induced by exposure to toxic metals. It is highly expressed in breast cancer cells. In this study, the eDNA encoding the human MT2A protein was expressed as glutathione S-transferase (GST) fusion protein in Escherichia coli.Recombinant MT2A proteins were loaded onto 12% sodium dodecylsulfate-polyacrylamide gel and separated by electrophoresis, the recombinant protein was visualized by Coomassie blue staining and the 33 kDa recombinant GST-MT2A fusion protein band was cut out from the gel. The gel slice was minced and used to generate polyclonal antisera. Immunization of rabbit against MT2A protein allowed the production of high titer polyclonal antiserum. This new polyclonal antibody recognized recombinant MT2A protein in Western blot analysis. This low-cost antibody will be useful for detection in various immuno-assays.

  20. Preliminary perspectives on DNA collection in anti-human trafficking efforts.

    Science.gov (United States)

    Katsanis, Sara H; Kim, Joyce; Minear, Mollie A; Chandrasekharan, Subhashini; Wagner, Jennifer K

    2014-01-01

    Forensic DNA methodologies have potential applications in the investigation of human trafficking cases. DNA and relationship testing may be useful for confirmation of biological relationship claims in immigration, identification of trafficked individuals who are missing persons, and family reunification of displaced individuals after mass disasters and conflicts. As these applications rely on the collection of DNA from non-criminals and potentially vulnerable individuals, questions arise as to how to address the ethical challenges of collection, security, and privacy of collected samples and DNA profiles. We administered a survey targeted to victims' advocates to gain preliminary understanding of perspectives regarding human trafficking definitions, DNA and sex workers, and perceived trust of authorities potentially involved in DNA collection. We asked respondents to consider the use of DNA for investigating adoption fraud, sex trafficking, and post-conflict child soldier cases. We found some key differences in perspectives on defining what qualifies as "trafficking." When we varied terminology between "sex worker" and "sex trafficking victim" we detected differences in perception on which authorities can be trusted. Respondents were supportive of the hypothetical models proposed to collect DNA. Most were favorable of DNA specimens being controlled by an authority outside of law enforcement. Participants voiced concerns focused on privacy, misuse of DNA samples and data, unintentional harms, data security, and infrastructure. These preliminary data indicate that while there is perceived value in programs to use DNA for investigating cases of human trafficking, these programs may need to consider levels of trust in authorities as their logistics are developed and implemented. PMID:25687341

  1. Anti-humanity in Food Habit%人类口舌也残忍

    Institute of Scientific and Technical Information of China (English)

    笑笑

    2010-01-01

    @@ 饮食这回事儿,吃饱肚子是功能之一,还有另外一种功能是弘扬文化.所以,有韩国总统亲自下厨为各国元首制作烤肉的新闻.但文化这东西极易变味儿,拿饮食来说,有人认为是文化的东西,有人就认为是反文化.你不信咱就梳理一下--

  2. Sophorolipids, Microbial Glycolipids with Anti-Human Immunodeficiency Virus and Sperm-Immobilizing Activities

    OpenAIRE

    Shah, Vishal; Doncel, Gustavo F.; Seyoum, Theodoros; Eaton, Kristin M.; Zalenskaya, Irina; Hagver, Rena; Azim, Abul; Gross, Richard

    2005-01-01

    The increased incidence of human immunodeficiency virus (HIV)/AIDS disease in women aged 15 to 49 years has identified the urgent need for a female-controlled, efficacious, and safe vaginal topical microbicide. To meet this challenge, sophorolipid (SL) produced by Candida bombicola and its structural analogs have been studied in this report for their spermicidal, anti-HIV, and cytotoxic activities. The sophorolipid diacetate ethyl ester derivative is the most potent spermicidal and virucidal ...

  3. Cloning and Sequence Analysis of Light Variable Region Gene of Anti-human Retinoblastoma Monoclonal Antibody

    Institute of Scientific and Technical Information of China (English)

    Xiufeng Zhong; Yongping Li; Shuqi Huang; Bo Ning; Chunyan Zhang; Jianliang Zheng; Guanguang Feng

    2002-01-01

    Purpose: To clone the variable region gene of light chain of monoclonal antibody against human retinoblastoma and to analyze the characterization of its nucleotide sequence as well as amino acid sequence.Methods: Total RNA was extracted from 3C6 hybridoma cells secreting specific monoclonal antibody(McAb)against human retinoblastoma(RB), then transcripted reversely into cDNA with olig-dT primers.The variable region of the light chain (VL) gene fragments was amplified using polymeerase chain reaction(PCR) and further cloned into pGEM(R) -T Easy vector. Then, 3C6 VL cDNA was sequenced by Sanger's method.Homologous analysis was done by NCBI BLAST.Results: The complete nucleotide sequence of 3C6 VL cDNA consisted of 321 bp encoding 107 amino acid residues, containing four workframe regions(FRs)and three complementarity-determining regions (CDRs) as well as the typical structure of two cys residues. The sequence is most homological to a member of the Vk9 gene family, and its chain utilizes the Jkl gene segment.Conclusion: The light chain variable region gene of the McAb against human RB was amplified successfully , which belongs to the Vk9 gene family and utilizes Vk-Jk1 gene rearrangement. This study lays a good basis for constructing a recombinant antibody and for making a new targeted therapeutic agents against retinoblastoma.

  4. Fabrication of Anti-human Cardiac Troponin I Immunogold Nanorods for Sensing Acute Myocardial Damage

    Science.gov (United States)

    Guo, Z. R.; Gu, C. R.; Fan, X.; Bian, Z. P.; Wu, H. F.; Yang, D.; Gu, N.; Zhang, J. N.

    2009-12-01

    A facile, rapid, solution-phase method of detecting human cardiac troponin I for sensing myocardial damage has been described using gold nanorods-based biosensors. The sensing is demonstrated by the distinct change of the longitudinal surface plasmon resonance wavelength of the gold nanorods to specific antibody-antigen binding events. For a higher sensitivity, the aspect ratio of gold nanorods is increased up to ca 5.5 by simply adding small amount of HCl in seed-mediated growth solution. Experimental results show that the detecting limit of the present method is 10 ng/mL. Contrast tests reveal that these gold nanorods-based plasmonic biosensors hold much higher sensitivity than that of conventionally spherical gold nanoparticles.

  5. Fabrication of Anti-human Cardiac Troponin I Immunogold Nanorods for Sensing Acute Myocardial Damage

    Directory of Open Access Journals (Sweden)

    Fan X

    2009-01-01

    Full Text Available Abstract A facile, rapid, solution-phase method of detecting human cardiac troponin I for sensing myocardial damage has been described using gold nanorods-based biosensors. The sensing is demonstrated by the distinct change of the longitudinal surface plasmon resonance wavelength of the gold nanorods to specific antibody–antigen binding events. For a higher sensitivity, the aspect ratio of gold nanorods is increased up to ca 5.5 by simply adding small amount of HCl in seed-mediated growth solution. Experimental results show that the detecting limit of the present method is 10 ng/mL. Contrast tests reveal that these gold nanorods-based plasmonic biosensors hold much higher sensitivity than that of conventionally spherical gold nanoparticles.

  6. POTENT INVITRO ANTI-HUMAN IMMUNODEFICIENCY VIRUS-1 ACTIVITY OF MODIFIED HUMAN SERUM ALBUMINS

    NARCIS (Netherlands)

    JANSEN, RW; MOLEMA, G; PAUWELS, R; SCHOLS, D; DECLERCQ, E; MEIJER, DKF

    1991-01-01

    A series of neoglycoproteins was synthesized by coupling of thiophosgene-activated p-aminophenyl derivatives [Biol. Cell. 47:95-110 (1983); J. Histochem. Cytochem. 32:1091-1094 (1984)] of various sugars to human serum albumin. The compounds were evaluated for their in vitro activity against human im

  7. Preliminary perspectives on DNA collection in anti-human trafficking efforts.

    Science.gov (United States)

    Katsanis, Sara H; Kim, Joyce; Minear, Mollie A; Chandrasekharan, Subhashini; Wagner, Jennifer K

    2014-01-01

    Forensic DNA methodologies have potential applications in the investigation of human trafficking cases. DNA and relationship testing may be useful for confirmation of biological relationship claims in immigration, identification of trafficked individuals who are missing persons, and family reunification of displaced individuals after mass disasters and conflicts. As these applications rely on the collection of DNA from non-criminals and potentially vulnerable individuals, questions arise as to how to address the ethical challenges of collection, security, and privacy of collected samples and DNA profiles. We administered a survey targeted to victims' advocates to gain preliminary understanding of perspectives regarding human trafficking definitions, DNA and sex workers, and perceived trust of authorities potentially involved in DNA collection. We asked respondents to consider the use of DNA for investigating adoption fraud, sex trafficking, and post-conflict child soldier cases. We found some key differences in perspectives on defining what qualifies as "trafficking." When we varied terminology between "sex worker" and "sex trafficking victim" we detected differences in perception on which authorities can be trusted. Respondents were supportive of the hypothetical models proposed to collect DNA. Most were favorable of DNA specimens being controlled by an authority outside of law enforcement. Participants voiced concerns focused on privacy, misuse of DNA samples and data, unintentional harms, data security, and infrastructure. These preliminary data indicate that while there is perceived value in programs to use DNA for investigating cases of human trafficking, these programs may need to consider levels of trust in authorities as their logistics are developed and implemented.

  8. Treatment of childhood aplastic anemia with antithymocyte globulin, management of side effects and long-term follow-up%抗胸腺细胞球蛋白治疗儿童再生障碍性贫血的临床研究

    Institute of Scientific and Technical Information of China (English)

    傅晓燕; 谢晓恬; 蒋莎义; 石苇; 邵越霞

    2011-01-01

    Objective To evaluate the efficacy of antithymocyte globulin (ATG) based immunosupression therapy for childhood aplastic anemia, to reduce the adverse effects and to observe the long-term outcome. Method Thirty-five children with aplastic anemia (AA) were enrolled in this study.Six of the cases had very severe AA (VSAA), 11 had severe AA (SAA)-Ⅰ, 8 had SAA-Ⅱ and 10 had moderate AA (MAA). All these patients were treated with ATG plus Cyclosporin A (CSA). The following measures were taken during the ATG therapy: infection of the patients had been controlled before ATG treatment. Comprehensive anti-allergic measures were implemented. Close attention was paid to the hemorrhage related with platelet reduction caused by ATG and severe infection of the patients. Result Shortly after the ATG usage, all the patients had a significant decrease of absolute peripheral lymphoblast count by more than 60 percent. With a mean follow-up time of 28 months, the total effective rate was 77.14% ( 27/35), significant response rate was 57.14%(20/35). There was no significant difference among VSAA, SAA and MAA groups in the response rate. Adverse reactions included the following:① 48.6% (17/35) patients presented mild anaphylactoid reaction during the first day of ATG treatment; ②42.9%(15/35) cases presented serum sickness 5-11 days after the last dose of ATG with a mean duration of 3. 6 days, all the patients were cured effectively with methylprednisolone; ③25.7% (9/35)patient's peripheral blood platelet count was reduced, might be caused by ATG, to below 10 × 109/L, but no patient had severe hemorrhagic complication after platelet transfusion was performed; ④22.9% ( 8/35 ) of patients got infection within a month after ATG therapy, including 3 cases with clinical septicemia, all the 3 cases recovered after antibiotics treatment. There was no ATG treatment-related death in this series. Conclusion ATG is a very effective therapy for children with SAA and MAA

  9. Screening, expression, and characterization of an anti-human oxidized low-density lipoprotein single-chain variable fragment.

    Science.gov (United States)

    Kumano-Kuramochi, Miyuki; Fujimura, Takashi; Komba, Shiro; Maeda-Yamamoto, Mari; Machida, Sachiko

    2016-09-01

    Increased levels of oxidized low-density lipoprotein (OxLDL) in the blood circulation are correlated with atherosclerosis. Monoclonal antibody-based detection systems have been reported for OxLDL. We identified novel single-chain variable fragments (scFvs) having affinity for human OxLDL and related ligands. We constructed an scFv library from nonimmunized human spleen mRNA. Two types (γ+κ and μ+λ) of scFv phage libraries were enriched by biopanning, and five scFv clones with affinity for OxLDL were identified. The γκ5 scFv, which showed the highest affinity for OxLDL, was cloned into pET-22b(+) and expressed in Escherichia coli BL21(DE3). γκ5, expressed as an inclusion body in BL21(DE3), was refolded and purified. The specificity and sensitivity of γκ5 were analyzed using enzyme-linked immunosorbent assays (ELISAs). The γκ5 scFv showed affinity for OxLDL and acetylated LDL. The sensitivity of γκ5 to low concentrations (1-2 μg/mL) of OxLDL was higher than that to AcLDL and LDL. Finally, we developed a sandwich ELISA using γκ5 and CTLD14 (a lectin-like OxLDL receptor-1 ligand recognition region), which allowed specific detection of OxLDL at a level below 0.1 μg/mL. Our results indicated that the γκ5 scFv was a promising molecule for the detection of modified LDL at very low concentrations. PMID:27038672

  10. Prospective isolation of mesenchymal stem cells from multiple mammalian species using cross-reacting anti-human monoclonal antibodies.

    Science.gov (United States)

    Rozemuller, Henk; Prins, Henk-Jan; Naaijkens, Benno; Staal, Jojet; Bühring, Hans-Jörg; Martens, Anton C

    2010-12-01

    Mesenchymal stem cells (MSCs) of human and nonhuman mammalian species are often studied for various applications in regenerative medicine research. These MSCs can be derived from human bone marrow (BM) and identified by their ability to form fibroblast-like colony forming units that develop into stromal like cells when expanded in culture. These cells are characterized by their spindle-shaped morphology, their characteristic phenotype (CD73(+), CD90(+), CD105(+), CD45⁻, and CD34⁻), and their ability to differentiate into cells of the osteogenic, adipogenic, and chondrogenic lineages. However, the identification and purification of MSCs from nonhuman mammalian species is hampered by the lack of suitable monoclonal antibodies (mAb). In this report, primary BM and cultured BM-derived MSCs of human and monkey, goat, sheep, dog, and pig were screened for cross-reactivity using a panel of 43 mAb, of which 22 react with either human BM mononuclear cells or cultured human MSCs. We found 7 mAb with specificity for CD271, MSCA-1 (W8B2 antigen), W4A5, CD56, W3C4 (CD349), W5C4, and 58B1, which showed interspecies cross-reactivity. These mAb proved to be useful for prospective sorting of MSCs from the BM of the 6 mammalian species studied as well as for the characterization of their cultured offspring. Flow sorting with the cross-reacting mAb resulted in up to 2400-fold enrichment of the clonogenic cell fraction (fibroblast-like colony forming units). This study provides an important contribution for the comparative prospective isolation of primary BM-MSCs and the characterization of cultured MSCs from multiple mammalian species for preclinical research. PMID:20367498

  11. Anti-Human Platelet Antigen-1a Immunoglobulin G Preparation Intended to Prevent Fetal and Neonatal Alloimmune Thrombocytopenia.

    Science.gov (United States)

    Weng, Ying-Jan; Husebekk, Anne; Skogen, Björn; Kjaer, Mette; Lin, Liang-Tzung; Burnouf, Thierry

    2016-01-01

    Fetal and neonatal alloimmune thrombocytopenia (FNAIT) is a severe disease that is caused by maternal alloantibodies generated during pregnancy or at delivery as a result of incompatibility between maternal and fetal human platelet antigens (HPAs) inherited from the father. Antibody-mediated immune suppression using anti-HPA-1a immunoglobulins is thought to be able to prevent FNAIT caused by HPA-1a. A fractionation process to prepare anti-HPA-1a immunoglobulin (Ig) G (IgG) from human plasma was therefore developed. Anti-HPA-1a plasma was obtained from volunteer mothers who underwent alloimmunization against HPA-1a during a previous pregnancy. Plasma was cryoprecipitated and the supernatant treated with caprylic acid and solvent/detergent (S/D), purified by chromatography, nanofiltered, concentrated, and sterile-filtered. The anti-HPA-1a immunoglobulin fraction was characterized for purity and safety. PAK12 and quantitative monoclonal antibody immobilization of platelet antigen (MAIPA) assays were used to detect anti-HPA-1a IgG. Hepatitis C virus (HCV) removal during nanofiltration was assessed by spiking experiments, using cell culture-derived reporter HCV and luciferase analysis. The caprylic acid treatment precipitated non-Ig proteins yielding a 90% pure Ig supernatant. S-HyperCel chromatography of the S/D-treated supernatant followed by HyperCel STAR AX provided high IgG recovery (>80%) and purity (>99.5%), and efficient IgA and IgM removal. Concentrations of complement factors C3 and C4 were < 0.5 and < 0.4 mg/dL, respectively. The final IgG could be nanofiltered on Planova 20N under conditions removing more than 3 log HCV infectivity to baseline mock infection level, and concentrated to ca. 30 g/L. Proteolytic activity and thrombin generation were low in the final fraction. The Pak12 and MAIPA assays showed good recovery of anti-HPA-1a throughout the process. Clinical-grade HPA-1a IgG can be prepared using a process compliant with current quality requirements opening perspectives for the prevention of FNAIT.

  12. Effective suppression of C5a-induced proinflammatory response using anti-human C5a repebody.

    Science.gov (United States)

    Hwang, Da-Eun; Choi, Jung-Min; Yang, Chul-Su; Lee, Joong-Jae; Heu, Woosung; Jo, Eun-Kyeong; Kim, Hak-Sung

    2016-09-01

    The strongest anaphylatoxin, C5a, plays a critical role in the proinflammatory responses, causing the pathogenesis of a number of inflammatory diseases including sepsis, asthma, and rheumatoid arthritis. Inhibitors of C5a thus have great potential as therapeutics for various inflammatory disorders. Herein, we present the development of a high-affinity repebody against human C5a (hC5a), which effectively suppresses the proinflammatory response. A repebody scaffold composed of leucine-rich repeat (LRR) modules was previously developed as an alternative protein scaffold. A repebody specifically binding to hC5a was selected through a phage display, and its affinity was increased up to 5 nM using modular engineering. The repebody was shown to effectively inhibit the production of C5a-induced proinflammatory cytokines by human monocytes. To obtain insight into a mode of action by the repebody, we determined its crystal structure in complex with hC5a. A structural analysis revealed that the repebody binds to the D1 and D3 regions of hC5a, overlapping several epitope residues with the hC5a receptor (hC5aR). It is thus likely that the repebody suppresses the hC5a-mediated immune response in monocytes by blocking the binding of hC5a to its receptor. The anti-hC5a repebody can be developed as a potential therapeutic for C5a-involved inflammatory diseases. PMID:27416759

  13. Anti-human Vascular Endothelial Growth Factor (VEGF) Antibody Selection for Immunohistochemical Staining of Proliferating Blood Vessels

    NARCIS (Netherlands)

    C.M. van der Loos; L.B. Meijer-Jorna; M.E.C. Broekmans; H.P.H.M. Ploegmakers; P. Teeling; O.J. de Boer; A.C. van der Wal

    2010-01-01

    Nine commercially available VEGF antibodies are investigated for their ability to immunostain vascular malformations (VM) with or without immature capillary proliferation. First, all antibodies were optimized for their performance in immunohistochemistry with placenta and colon adenocarcinoma as pos

  14. A Unique Report: Development of Super Anti-Human IgG Monoclone with Optical Density Over Than 3

    Directory of Open Access Journals (Sweden)

    Leili Aghebati Maleki

    2013-08-01

    Full Text Available Purpose: Monoclonal antibodies and related conjugates are key reagents used in biomedical researches as well as, in treatment, purification and diagnosis of infectious and non- infectious diseases. Methods: Balb/c mice were immunized with purified human IgG. Spleen cells of the most immune mouse were fused with SP2/0 in the presence of Poly Ethylene Glycol (PEG. Supernatant of hybridoma cells was screened for detection of antibody by ELISA. Then, the sample was assessed for cross-reactivity with IgM & IgA by ELISA and confirmed by immunoblotting. The subclasses of the selected mAbs were determined. The best clone was injected intraperitoneally to some pristane-injected mice. Anti-IgG mAb was purified from the animals' ascitic fluid by Ion exchange chromatography and then, mAb was conjugated with HRP. Results: In the present study, over than 50 clones were obtained that 1 clone had optical density over than 3. We named this clone as supermonoclone which was selected for limiting dilution. The result of the immunoblotting, showed sharp band in IgG position and did not show any band in IgM&IgA position. Conclusion: Based on the findings of this study, the conjugated monoclonal antibody could have application in diagnosis of infectious diseases like Toxoplasmosis, Rubella and IgG class of other infectious and non- infectious diseases.

  15. THE EUKARYOTIC EXPRESSION OF HUMAN PERFORIN PROTEIN AND THE ESTABLISHMENT OF HYBRIDOMAS PRODUCING ANTI-HUMAN PERFORIN PROTEIN

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    Objective To prepare the monoclonal antibody against human perforin(HP). Methods Recombinant eukaryotic expression plasmid pCDM8-HP was extracted and purified, and the BALB/C mice were immunized with the plasmid. The hybridomas producing anti-HP McAbs were established by using hybridoma technique, then the specificity of the McAbs was identified by using immunocytochemical technique and Western blot. Results Three hybridoma cell lines secreting McAbs against human perforin were established, and the three McAbs showed positive only with LAK cells containing human perforin protein, and showed negative with inactive human peripheral blood lymphocytes (PBLs). The subclasses of the three McAbs were determined as lgG2bK. Western blot results showed that the three McAbs recognized a specific band of LAK cell lysateds with molecular weight of 70.0Kd. Conclusion The three hybridoma cell lines secreting McAbs against human perforin were established and the secreted McAbs were specific.

  16. Anti-Human Platelet Antigen-1a Immunoglobulin G Preparation Intended to Prevent Fetal and Neonatal Alloimmune Thrombocytopenia

    Science.gov (United States)

    Weng, Ying-Jan; Husebekk, Anne; Skogen, Björn; Kjaer, Mette; Lin, Liang-Tzung; Burnouf, Thierry

    2016-01-01

    Fetal and neonatal alloimmune thrombocytopenia (FNAIT) is a severe disease that is caused by maternal alloantibodies generated during pregnancy or at delivery as a result of incompatibility between maternal and fetal human platelet antigens (HPAs) inherited from the father. Antibody-mediated immune suppression using anti-HPA-1a immunoglobulins is thought to be able to prevent FNAIT caused by HPA-1a. A fractionation process to prepare anti-HPA-1a immunoglobulin (Ig) G (IgG) from human plasma was therefore developed. Anti-HPA-1a plasma was obtained from volunteer mothers who underwent alloimmunization against HPA-1a during a previous pregnancy. Plasma was cryoprecipitated and the supernatant treated with caprylic acid and solvent/detergent (S/D), purified by chromatography, nanofiltered, concentrated, and sterile-filtered. The anti-HPA-1a immunoglobulin fraction was characterized for purity and safety. PAK12 and quantitative monoclonal antibody immobilization of platelet antigen (MAIPA) assays were used to detect anti-HPA-1a IgG. Hepatitis C virus (HCV) removal during nanofiltration was assessed by spiking experiments, using cell culture-derived reporter HCV and luciferase analysis. The caprylic acid treatment precipitated non-Ig proteins yielding a 90% pure Ig supernatant. S-HyperCel chromatography of the S/D-treated supernatant followed by HyperCel STAR AX provided high IgG recovery (>80%) and purity (>99.5%), and efficient IgA and IgM removal. Concentrations of complement factors C3 and C4 were HPA-1a throughout the process. Clinical-grade HPA-1a IgG can be prepared using a process compliant with current quality requirements opening perspectives for the prevention of FNAIT. PMID:27627660

  17. Biological Validation of Plant-derived Anti-human Colorectal Cancer Monoclonal Antibody CO17-1A

    NARCIS (Netherlands)

    Jamal, Arshad; Ahn, Mi-Hyun; Song, Mira; Oh, Eun-Yi; Hong, Juyeon; Choo, Young-Kug; Ko, Kinarm; Han, Yeon Soo; Oh, Seung Han; Van Der Linden, Joke; Leusen, Jeanette H. W.; Ko, Kisung

    2009-01-01

    We validated expression and biological activities of plant-derived monoclonal antibody (MAb(P)) CO17-1A for its efficacy in cancer immunotherapy. PCR and immunoblot analyses demonstrated insertion and expression of heavy and light chains of MAb CO17-1A in transgenic plants, respectively. Confocal an

  18. Purification, crystallization and initial crystallographic characterization of the Ginkgo biloba 11S seed globulin ginnacin

    Energy Technology Data Exchange (ETDEWEB)

    Jin, Tengchuan; Chen, Yu-Wei; Howard, Andrew; Zhang, Yu-Zhu, E-mail: zhangy@iit.edu [Department of Biological, Chemical and Physical Sciences, Illinois Institute of Technology, Chicago, IL 60616 (United States)

    2008-07-01

    The crystallization of ginnacin, the 11S seed storage protein from G. biloba, is reported. Ginkgo biloba, a well known ‘living fossil’ native to China, is grown worldwide as an ornamental shade plant. Medicinal and nutritional uses of G. biloba in Asia have a long history. However, ginkgo seed proteins have not been well studied at the biochemical and molecular level. In this study, the G. biloba 11S seed storage protein ginnacin was purified by sequential anion-exchange and gel-filtration chromatography. A crystallization screen was performed and well diffracting single crystals were obtained by the vapor-diffusion method. A molecular-replacement structural solution has been obtained. There are six protomers in an asymmetric unit. Structure refinement is currently in progress.

  19. Sex hormones, sex hormone binding globulin, and vertebral fractures in older men.

    Science.gov (United States)

    Cawthon, Peggy M; Schousboe, John T; Harrison, Stephanie L; Ensrud, Kristine E; Black, Dennis; Cauley, Jane A; Cummings, Steven R; LeBlanc, Erin S; Laughlin, Gail A; Nielson, Carrie M; Broughton, Augusta; Kado, Deborah M; Hoffman, Andrew R; Jamal, Sophie A; Barrett-Connor, Elizabeth; Orwoll, Eric S

    2016-03-01

    The association between sex hormones and sex hormone binding globin (SHBG) with vertebral fractures in men is not well studied. In these analyses, we determined whether sex hormones and SHBG were associated with greater likelihood of vertebral fractures in a prospective cohort study of community dwelling older men. We included data from participants in MrOS who had been randomly selected for hormone measurement (N=1463, including 1054 with follow-up data 4.6years later). Major outcomes included prevalent vertebral fracture (semi-quantitative grade≥2, N=140, 9.6%) and new or worsening vertebral fracture (change in SQ grade≥1, N=55, 5.2%). Odds ratios per SD decrease in sex hormones and per SD increase in SHBG were estimated with logistic regression adjusted for potentially confounding factors, including age, bone mineral density, and other sex hormones. Higher SHBG was associated with a greater likelihood of prevalent vertebral fractures (OR: 1.38 per SD increase, 95% CI: 1.11, 1.72). Total estradiol analyzed as a continuous variable was not associated with prevalent vertebral fractures (OR per SD decrease: 0.86, 95% CI: 0.68 to 1.10). Men with total estradiol values ≤17pg/ml had a borderline higher likelihood of prevalent fracture than men with higher values (OR: 1.46, 95% CI: 0.99, 2.16). There was no association between total testosterone and prevalent fracture. In longitudinal analyses, SHBG (OR: 1.42 per SD increase, 95% CI: 1.03, 1.95) was associated with new or worsening vertebral fracture, but there was no association with total estradiol or total testosterone. In conclusion, higher SHBG (but not testosterone or estradiol) is an independent risk factor for vertebral fractures in older men.

  20. Induction of procalcitonin in liver transplant patients treated with anti-thymocyte globulin

    Science.gov (United States)

    Zazula, Roman; Prucha, Miroslav; Tyll, Tomas; Kieslichova, Eva

    2007-01-01

    Introduction The aim of this study was to compare the early postoperative kinetics of procalcitonin (PCT) and C-reactive protein (CRP) serum levels in patients undergoing orthotopic liver transplantation (OLTx) with different immunosuppressive regimens. Methods PCT and CRP serum concentrations were measured in a group of 28 OLTx recipients before induction of anesthesia, at 4 and 8 hours following graft reperfusion, and daily until postoperative day 4. The same parameters were determined in 12 patients undergoing liver resection without conjunctive immunosuppressive therapy. Summary data are expressed as medians and ranges. Two-tailed nonparametric tests were performed and considered significant at p values of less than 0.05. Results The highest serum levels of PCT (median 3.0 ng/mL, minimum 1.4 ng/mL, maximum 13.9 ng/mL) were found in patients after OLTx without ATG therapy, on postoperative day 1. In patients with ATG administration, PCT levels were highly increased on postoperative day 1 (median 53.0 ng/mL, minimum 7.9 ng/mL, maximum 249.1 ng/mL). Thereafter, PCT values continuously decreased independently of further ATG administration in both groups of patients. No evidence of infection was present in either group. In 12 patients undergoing liver resection, peak serum PCT levels did not exceed 3.6 ng/mL. CRP serum levels in a group of patients with and without ATG therapy increased significantly on postoperative day 1, followed by a decrease. The highest levels of CRP were found in patients after liver resection on postoperative day 2 and decreased thereafter. Conclusion ATG administration to patients with OLTx is associated with an increase in serum PCT levels, with peak values on postoperative day 1, and this was in the absence of any evidence of infection. The results of this study indicate that ATG immunosuppressive therapy is a stimulus for the synthesis of PCT. PMID:18088403

  1. Sex hormone binding globulin and incident Alzheimer's disease in elderly men and women

    NARCIS (Netherlands)

    Muller, Majon; Schupf, Nicole; Manly, Jennifer J.; Mayeux, Richard; Luchsinger, Jose A.

    2010-01-01

    It has been suggested that low levels of estradiol and testosterone increase dementia risk. However, results of the existing observational studies examining associations of endogenous sex hormones with cognition and dementia are conflicting. A possible explanation for these inconsistent findings cou

  2. Chronic Inflammatory Demyelinating Polyneuropathy With Diabetes Mellitus Is Responsive To Intravenous Immune Globulin; Case Report

    OpenAIRE

    Koca, Süleyman Serdar; YOLDAŞ, Tahir K.; ÖZKAN, Yusuf; GÜNAY, İzzettin; DÖNDER, Emir

    2006-01-01

    Chronic demyelinating polyneuropathy (CIDP) is a disease which has different treatment modality like immunomodulatory method and have good response to treatment than the other peripheral neuropathy. We have established a patient with CIDP female 68 years old and had a type 2 diabetes mellitus diagnosis for 16 years. She treated with intravenous immunoglobuline (0.5 mg/kg/day) for five days and four weeks intervals at six months. This case has showed that the autoimmune neuropathy should keep ...

  3. Enhanced Activity of Topical Hydrocortisone by Competitive Binding of Corticosteroid-Binding Globulin.

    Science.gov (United States)

    Bodor, Erik T; Wu, Whei-Mei; Chandran, V Ravi; Bodor, Nicholas

    2016-09-01

    Atopic dermatitis of sensitive areas such as the face, particularly in children, is a difficult disease to treat as the standard therapeutic, topical steroids, is contraindicated for this application in children. Hydrocortisone (HC) can be used in these instances because it has been shown to be safe, but is often ineffective as it is a relatively weak steroid, especially at over-the-counter concentrations. To enhance the local topical activity of HC, the terminal inactive metabolite of prednisolone, Δ(1)-cortienic acid (Δ(1)-CA), is added to HC, as Δ(1)-CA preferentially binds transcortin, liberating more HC to elicit its therapeutic effect. Skin blanching studies, which are used to evaluate the potency of topical steroids, were employed to assess the ability of Δ(1)-CA to enhance the activity of HC. The results demonstrate that Δ(1)-CA, when applied in combination with HC, does indeed potentiate the vasoconstriction effect of topically applied HC, while having no effect alone. Thus, addition of the inert prednisolone metabolite Δ(1)-CA can increase the therapeutic effect of over-the-counter concentrations of HC when applied topically. PMID:27179671

  4. Comparison of dexamethasone and Anti-D Immune globulin for immune thrombocytopenia purpura in children

    OpenAIRE

    Abdollah Banihashem; Hamid Farhangi; Mojtaba Mousavi Bazaz; Zahra Badiee; Ali Ghasemi; Sara Hesari

    2014-01-01

    Different therapeutic options in children with immune thrombocytopenic purpura include observation alone, periodic treatment with corticosteroids, intravenous immunoglobulin (IVIG) or anti-D, chronic administration of immunosuppressive agents, and splenectomy. Preference of the type of therapy depends on the degree of thrombocytopenia and clinical bleeding manifestations. Dexamethasone is safe but its side effects are the main disadvantages for its usage. Anti-D is more expensive than dexamet...

  5. Treatment of D alloimmunization in pregnancy with plasmapheresis and intravenous immune globulin: case report.

    Science.gov (United States)

    Fernández Alba, Juan J; León, Raquel; González-Macías, Carmen; Paz, Antonio; Prado, Fabiana; Moreno, Luis J; Torrejón, Rafael

    2014-08-01

    The prevalence of D alloimmunization occurs between 0.15% and 0.4%. The anti-D can cross the placenta and cause hemolysis and fetal anemia. At present, a Doppler study of the middle cerebral artery allows the monitoring of the degree of fetal anemia. The treatment in cases of moderate to severe anemia in fetuses of less than 34-35 weeks of gestation is intrauterine transfusion via cordocentesis. However, with high titers of anti-D, in the absence of fetal anemia it is possible to modulate the maternal immune response by plasmapheresis and intravenous immunoglobulin administration. We present a case report of an Rh(D) alloimmunized pregnancy treated with plasmapheresis followed by intravenous immunoglobulin administration. We performed a caesarean section at 31 weeks, 5 days of gestation. The hemoglobin at birth was 13.8 g/dl and hematocrit 40.8%. Intrauterine transfusion was not necessary. PMID:25312036

  6. The role of high-dose immune globulin intravenous in the treatment of dermatomyositis.

    Science.gov (United States)

    Dalakas, Marinos C

    2006-04-01

    Dermatomyositis (DM) is a complement-mediated microangiopathy affecting skin and muscle resulting in skin abnormalities, including subcutenous calcifications, muscle weakness and disability. The disease generally responds to steroids or immunosuppressive drugs, but a number of patients are resistant or partially responsive to these therapies, prompting us to examine the efficacy of IVIg. A double-blind placebo-controlled study demonstrated that IVIg is very effective in improving both the muscle strength and the skin rash. The clinical benefit, which was impressive in patients with early disease, was associated with improvement in the muscle cytoarchitecture. Quantitative histological studies in repeated muscle biopsies showed a statistically significant increase in the size of muscle fibers and the number of capillaries with normalization of the capillary diameter. Resolution of the aberrant immunopathological parameters, including interception of complement activation products and downregulation of T cells, ICAM-I, VCAM, TGF-beta and MHC-I molecules, was also noted. Further, a number of immunoregulatory and structural genes were modified in the patients' muscle biopsies after therapy. The study concluded that IVIg is an effective second-line therapy for patients with DM incompletely responding to steroids.

  7. Treatment of Cytomegalovirus Infection with Cidofovir and CMV Immune Globulin in a Lung Transplant Recipient

    Directory of Open Access Journals (Sweden)

    Heinrike Wilkens

    2016-01-01

    Full Text Available Cytomegalovirus (CMV infection after lung transplantation is associated with increased risk for pneumonitis and bronchiolitis obliterans as well as allograft rejection and opportunistic infections. Ganciclovir is the mainstay of prophylaxis and treatment but CMV infections can be unresponsive. Apart from direct antiviral drugs, CMV immunoglobulin (CMVIG preparations may be considered but are only licensed for prophylaxis. A CMV-seronegative 42-year-old man with cystic fibrosis received a lung from a CMV-seropositive donor. Intravenous ganciclovir prophylaxis was delayed until day 12 due to acute postoperative renal failure and was accompanied by five doses of CMVIG (10 g. By day 16, CMV-DNA was detectable and rising; CMV-specific T-cells were undetectable. Switch from ganciclovir to foscarnet prompted a transient decrease in CMV viral load, but after increasing again to reach 3600 copies/mL foscarnet was changed to intravenous cidofovir and CMVIG was restarted. CMV load continued to fluctuate and declined slowly, whereas CMV-specific T-cells were detected five months later and increased thereafter. At last follow-up, the patient was in very good clinical condition with no evidence of bronchiolitis obliterans. No side effects of this treatment were observed. In this hard-to-treat case, the combination of cidofovir with off-label use of CMVIG contributed to a successful outcome.

  8. Partition of volatile compounds in pea globulin-maltodextrin aqueous two-phase system.

    Science.gov (United States)

    Nguyen, Thanh Dat; Lafarge, Céline; Murat, Chloé; Mession, Jean-Luc; Cayot, Nathalie; Saurel, Rémi

    2014-12-01

    This study is based on the assumption that the off-flavour of pea proteins might be decreased using the retention of volatile compounds by a mixture with another biopolymer. The partition of volatile compounds in an aqueous system containing pea protein and maltodextrins was followed under thermodynamic incompatibility conditions. Firstly, the phase diagram of the system was established. Then, the partition of aroma compounds between the phase rich in protein and the phase rich in maltodextrin was measured by SPME-GC-MS. There was a transfer of volatile compounds during phase separation. Variations of pH were also used to vary the retention of volatile compounds by proteins. The concentration of volatile compounds in protein solution at pH 2.4 was higher than at pH 7.2. It was possible to increase the transfer of volatile compounds from the phase rich in protein to the phase rich in maltodextrin using the effect of pH on protein denaturation.

  9. Purification, crystallization and initial crystallographic characterization of the Ginkgo biloba 11S seed globulin ginnacin

    International Nuclear Information System (INIS)

    The crystallization of ginnacin, the 11S seed storage protein from G. biloba, is reported. Ginkgo biloba, a well known ‘living fossil’ native to China, is grown worldwide as an ornamental shade plant. Medicinal and nutritional uses of G. biloba in Asia have a long history. However, ginkgo seed proteins have not been well studied at the biochemical and molecular level. In this study, the G. biloba 11S seed storage protein ginnacin was purified by sequential anion-exchange and gel-filtration chromatography. A crystallization screen was performed and well diffracting single crystals were obtained by the vapor-diffusion method. A molecular-replacement structural solution has been obtained. There are six protomers in an asymmetric unit. Structure refinement is currently in progress

  10. 76 FR 14413 - Risk Mitigation Strategies To Address Potential Procoagulant Activity in Immune Globulin...

    Science.gov (United States)

    2011-03-16

    ...) pathophysiology of arterial and venous thrombosis in this context; (3) research to identify specific procoagulant...) the role of activated Coagulation Factor XIa in IGIV-associated thrombosis; (6) test methods...

  11. Secular decline in male testosterone and sex hormone binding globulin serum levels in Danish population surveys

    DEFF Research Database (Denmark)

    Andersson, Anna-Maria; Jensen, Tina K; Juul, Anders;

    2007-01-01

    Adverse secular trends in male reproductive health have been reported to be reflected in increased testicular cancer risk and decreased semen quality in more recently born men. These secular trends may also be reflected by changes in Leydig cell function....

  12. Cholesterol crystal binding of biliary immuno globulin A: visualization by fluorescence light microscopy

    Institute of Scientific and Technical Information of China (English)

    Frank Lammert; Stefan Sudfetd; Norbert Busch; Siegfried Matern

    2001-01-01

    AIM To assess potential contributions of biliary IgA for crystal agglomeration into gallstones, we visualized cholesterol crystal binding of biliary IgA.METHODS Crystal-binding biliary proteins were extracted from human gallbladder bile using lectin affinity chromatography. Biliary IgA was isolated from the bound protein fraction by immunoaffinity chromatography. Pure cholesterol monohydrate crystals were incubated with biliary IgA and fluoresceine isothiocyanate (FITC)-conjugated anti-lgA at 37C. Samples were examined under polarizing and fluorescence light microscopy with digital image processing.RESULTS Binding of biliary IgA to cholesterol monohydrate crystals could be visualized with FITC-conjugated anti-lgA antibodies. Peak fluorescence occurred at crystal edges and dislocations. Controls without biliary IgA or with biliary IgG showed no significant fluorescence.CONCLUSION Fluorescence light microscopy provided evidence for cholesterol crystal binding of biliary IgA. Cholesterol crystalbinding proteins like IgA might be important mediators of crystal agglomeration and growth of cholesterol gallstones by modifying the evolving crystal structures in vivo.

  13. "Bound" globulin in the skin of patients with chronic discoid lupus erythematosus and systemic lupus erythematosus

    NARCIS (Netherlands)

    Cormane, R.H.

    1964-01-01

    In what respect chronic discoid lupus erythematosus is related to systemic lupus erythematosus is still uncertain. In discoid lupus the lupus-erythematosus (L.E.) phenomenon is negative, and the history does not suggest vascular lesions or involvement of serous membranes. In both diseases the pathog

  14. Comparative analysis of bilateral memoranda on anti-human trafficking cooperation between Thailand and three neighboring countries : what do the origin and the destination states agree upon?

    OpenAIRE

    Yamada, Miwa

    2012-01-01

    In order to prevent, suppress and punish human trafficking, bilateral agreements between origin of victim countries and destination countries are crucial, because their cooperation involves cross-border activities such as repatriation of victims, extradition of criminals and information-sharing. This article analyzes three bilateral legal instruments between The Government of The Kingdom of Thailand and her three neighboring countries, namely The Royal Government of Cambodia, The Government o...

  15. Large Scale Generation and Characterization of Anti-Human IgA Monoclonal Antibody in Ascitic Fluid of Balb/c Mice

    Directory of Open Access Journals (Sweden)

    Fatemeh Ezzatifar

    2015-03-01

    Full Text Available Purpose: Monoclonal antibodies are potentially powerful tools used in biomedical research, diagnosis, and treatment of infectious diseases and cancers. The monoclonal antibody against Human IgA can be used as a diagnostic application to detect infectious diseases. The aim of this study was to improve an appropriate protocol for large-scale production of mAbs against IgA. Methods: For large-scale production of the monoclonal antibody, hybridoma cells that produce monoclonal antibodies against Human IgA were injected intraperitoneally into Balb/c mice that were previously primed with 0.5 ml Pristane. After ten days, ascitic fluid was harvested from the peritoneum of each mouse. The ELISA method was carried out for evaluation of the titration of produced mAbs. The ascitic fluid was investigated in terms of class and subclass by a mouse mAb isotyping kit. MAb was purified from the ascitic fluid by ion exchange chromatography. The purity of the monoclonal antibody was confirmed by SDS-PAGE, and the purified monoclonal antibody was conjugated with HRP. Results: Monoclonal antibodies with high specificity and sensitivity against Human IgA were prepared by hybridoma technology. The subclass of antibody was IgG1 and its light chain was the kappa type. Conclusion: This conjugated monoclonal antibody could have applications in designing ELISA kits in order to diagnose different infectious diseases such as toxoplasmosis and H. Pylori.

  16. Anti-Human Immunodeficiency Virus Type 1 Microbicide Cellulose Acetate 1,2-Benzenedicarboxylate in a Human In Vitro Model of Vaginal Inflammation

    OpenAIRE

    Fichorova, R. N.; Zhou, F.(Central China Normal University, Wuhan, China); Ratnam, V.; Atanassova, V.; Jiang, S; Strick, N; Neurath, A R

    2005-01-01

    The sexual transmission of human immunodeficiency virus type 1 (HIV-1) is facilitated by inflammation and related epithelial barrier perturbation. Microbicides for vaginal applications are currently being developed to reduce the risk of HIV-1 transmission. However, little is known about their interference with epithelial immune function. In recent clinical trials, nonoxynol-9 (N-9), a virucide with a long history of intravaginal use as a contraceptive, failed to protect against HIV-1 possibly...

  17. Factors associated with anti-human leukocyte antigen antibodies in patients supported with continuous-flow devices and effect on probability of transplant and post-transplant outcomes

    DEFF Research Database (Denmark)

    Alba, Ana C; Tinckam, Kathryn; Foroutan, Farid;

    2015-01-01

    sensitized (PRA > 80%). Age, female sex, and pre-VAD PRA were independently associated with post-VAD PRA. A 10-year increase in age was associated with a 5% decrease in post-VAD PRA (p = 0.03). Post-VAD PRA was 19% higher in women vs men (p increase in pre-VAD PRA was associated with a 4...... with adverse post-transplant outcomes. CONCLUSIONS: Younger age, female sex, and pre-VAD PRA were independent predictors of elevated PRA post-VAD. Higher PRA was significantly associated with lower transplant probability but not increased rejection, graft failure, or death after transplant.......BACKGROUND: One major disadvantage of ventricular assist device (VAD) therapy is the development of human-leukocyte antigen (HLA) antibodies. We aimed to identify factors associated with HLA antibodies during continuous flow (CF)-VAD support and assess the effect on transplant probability...

  18. Induction of anti-human immunodeficiency virus (HIV) neutralizing antibodies in rabbits immunized with recombinant HIV--hepatitis B surface antigen particles.

    OpenAIRE

    Michel, M L; M. Mancini; Sobczak, E.; Favier, V.; Guetard, D; Bahraoui, E M; Tiollais, P

    1988-01-01

    Fragments of the human immunodeficiency virus (HIV) envelope coding region have been fused with the hepatitis B virus envelope middle protein. In this system, HIV antigenic determinants are exposed at the surface of a highly antigenic structure, the hepatitis B surface antigen particle. Immunization of rabbits with these particles elicited antibodies directed against both parts of the hybrid protein. One of the rabbit antisera not only exhibited a neutralizing effect on the original HIV1 isol...

  19. Murine leukemia virus-based Tat-inducible long terminal repeat replacement vectors: a new system for anti-human immunodeficiency virus gene therapy.

    Science.gov (United States)

    Cannon, P M; Kim, N; Kingsman, S M; Kingsman, A J

    1996-11-01

    We have constructed new murine leukemia virus (MLV)-based vectors (TIN vectors) which, following integration, contain human immunodeficiency virus (HIV) type 1 U3 and R sequences in place of the MLV U3 and R regions. This provides, for the first time, single transcriptional unit retroviral vectors under the control of Tat. TIN vectors have several advantages for anti-HIV gene therapy applications. PMID:8892960

  20. Fluorometric determination of 2'-beta-fluoro-2',3'-dideoxyadenosine 5'-triphosphate, the active metabolite of a new anti-human immunodeficiency virus drug, in human lymphocytes.

    Science.gov (United States)

    Dai, F; Kelley, J A; Zhang, H; Malinowski, N; Kavlick, M F; Lietzau, J; Welles, L; Yarchoan, R; Ford, H

    2001-01-01

    A sensitive precolumn derivatization method has been developed to measure the 5'-triphosphate of 2'-beta-fluoro-2',3'-dideoxyadenosine (F-ddA, lodenosine), a new anti-HIV drug, in human lymphocytes by HPLC using fluorescence detection. Reaction of chloroacetaldehyde with F-ddA triphosphate in extracts from human lymphocytes produces a highly fluorescent etheno adduct. This derivative is then separated and quantitated by reverse-phase paired-ion chromatography. Degradation of natural nucleic acid ribosides, such as ATP, using periodate oxidation simplifies the chromatogram and minimizes interference with detection of the target analyte. This method, modeled using cultured MOLT-4 T-lymphocytes, achieves a linear detector response for peak area measurements over the range 2.5 to 22.5 pmol (50-450 nM using 50 microl sample). Analyte recovery is greater than 90%, and the method achieves a limit of detection and limit of quantitation of 1.4 and 2.5 pmol per HPLC injection (50 microl sample containing cellular extract from 2.5 x 10(6) cells), respectively. Application of this method to measure F-ddATP in peripheral blood mononuclear cells from HIV-infected patients treated with F-ddA at 3.2 mg/kg twice daily for 22 days shows F-ddATP levels which range from 1.5 to 3.5 pmol/10(6) cells. PMID:11141306

  1. In Vitro Preclinical Testing of Nonoxynol-9 as Potential Anti-Human Immunodeficiency Virus Microbicide: a Retrospective Analysis of Results from Five Laboratories

    Science.gov (United States)

    Beer, Brigitte E.; Doncel, Gustavo F.; Krebs, Fred C.; Shattock, Robin J.; Fletcher, Patricia S.; Buckheit, Robert W.; Watson, Karen; Dezzutti, Charlene S.; Cummins, James E.; Bromley, Ena; Richardson-Harman, Nicola; Pallansch, Luke A.; Lackman-Smith, Carol; Osterling, Clay; Mankowski, Marie; Miller, Shendra R.; Catalone, Bradley J.; Welsh, Patricia A.; Howett, Mary K.; Wigdahl, Brian; Turpin, Jim A.; Reichelderfer, Patricia

    2006-01-01

    The first product to be clinically evaluated as a microbicide contained the nonionic surfactant nonoxynol-9 (nonylphenoxypolyethoxyethanol; N-9). Many laboratories have used N-9 as a control compound for microbicide assays. However, no published comparisons of the results among laboratories or attempts to establish standardized protocols for preclinical testing of microbicides have been performed. In this study, we compared results from 127 N-9 toxicity and 72 efficacy assays that were generated in five different laboratories over the last six years and were performed with 14 different cell lines or tissues. Intra-assay reproducibility was measured at two-, three-, and fivefold differences using standard deviations. Interassay reproducibility was assessed using general linear models, and interaction between variables was studied using step-wise regression. The intra-assay reproducibility within the same N-9 concentration, cell type, assay duration, and laboratory was consistent at the twofold level of standard deviations. For interassay reproducibility, cell line, duration of assay, and N-9 concentration were all significant sources of variability (P 24 h) and short (<2 h) exposures of cells to N-9 showed variability, while assays with 4 to 8 h of N-9 exposure gave results that were not significantly different. This is the first analysis to compare preclinical N-9 toxicity levels that were obtained by different laboratories using various protocols. This comparative work can be used to develop standardized microbicide testing protocols that will help advance potential microbicides to clinical trials. PMID:16436731

  2. Induction of humoral and cell-mediated anti-human immunodeficiency virus (HIV) responses in HIV sero-negative volunteers by immunization with recombinant gp160.

    Science.gov (United States)

    Kovacs, J A; Vasudevachari, M B; Easter, M; Davey, R T; Falloon, J; Polis, M A; Metcalf, J A; Salzman, N; Baseler, M; Smith, G E

    1993-01-01

    Development of an effective vaccine for prevention of infection with HIV would provide an important mechanism for controlling the AIDS epidemic. In the current study, the first clinical trial of a candidate HIV-1 vaccine initiated in the United States, the safety and immunogenicity of escalating doses (10-1,280 micrograms) of recombinant gp160 (rgp160), were evaluated in 138 HIV-negative volunteers. Maximal antibody responses, as evaluated by ELISA, were seen after immunization with three doses of 1,280 micrograms rgp160. Responses to some specific epitopes of HIV gp160, including the second conserved domain and the CD4 binding site, were seen more frequently than after natural infection. Neutralizing antibodies to the homologous HIV strain, but not heterologous strains, were induced by this regimen. Blastogenic responses to rgp160 were seen in most volunteers receiving at least two doses of > or = 20 micrograms. These envelope-specific T cell responses were also seen against heterologous strains of HIV. No major adverse reactions were seen after immunization. Thus, rgp160 is a safe and immunogenic candidate HIV vaccine; further studies are needed to determine if it will provide any clinical benefit in preventing HIV infection. Images PMID:7688766

  3. Large Scale Generation and Characterization of Anti-Human CD34 Monoclonal Antibody in Ascetic Fluid of Balb/c Mice

    Directory of Open Access Journals (Sweden)

    Koushan Sineh sepehr

    2013-02-01

    Full Text Available Purpose: Monoclonal antibodies or specific antibodies are now an essential tool of biomedical research and are of great commercial and medical value. The purpose of this study was to produce large scale of monoclonal antibody against CD34 in order to diagnostic application in leukemia and purification of human hematopoietic stem/progenitor cells. Methods: For large scale production of monoclonal antibody, hybridoma cells that produce monoclonal antibody against human CD34 were injected into the peritoneum of the Balb/c mice which have previously been primed with 0.5 ml Pristane. 5 ml ascitic fluid was harvested from each mouse in two times. Evaluation of mAb titration was assessed by ELISA method. The ascitic fluid was examined for class and subclasses by ELISA mouse mAb isotyping Kit. mAb was purified from ascitic fluid by affinity chromatography on Protein A-Sepharose. Purity of monoclonal antibody was monitored by SDS -PAGE and the purified monoclonal antibody was conjugated with FITC. Results: Monoclonal antibodies with high specificity and sensitivity against human CD34 by hybridoma technology were prepared. The subclass of antibody was IgG1 and its light chain was kappa. Conclusion: The conjugated monoclonal antibody could be a useful tool for isolation, purification and characterization of human hematopoietic stem cells.

  4. Priming of Anti-Human Immunodeficiency Virus (HIV) CD8^+ Cytotoxic T Cells in vivo by Carrier-Free HIV Synthetic Peptides

    Science.gov (United States)

    Hart, Mary Kate; Weinhold, Kent J.; Scearce, Richard M.; Washburn, Eileen M.; Clark, Cynthia A.; Palker, Thomas J.; Haynes, Barton F.

    1991-11-01

    The generation of antiviral cytotoxic T lymphocytes (CTLs) is a critical component of the immune response to viral infections. A safe and nontoxic vaccine for AIDS would optimally use a carrier-free synthetic peptide immunogen containing only components of HIV necessary for induction of protective immune responses. We report that hybrid synthetic peptides containing either a HIV envelope gp120 T-cell determinant (T1) or the envelope gp41 fusion domain (F) N-terminal to HIV CTL determinants are capable of priming murine CD8^+, major histocompatibility complex class I-restricted anti-HIV CTLs in vivo. These data demonstrate that carrier-free, nonderivatized synthetic peptides can be used in vivo to induce anti-HIV CTL responses.

  5. Evaluation of the Long-Term Anti-Human Papillomavirus 6 (HPV6), 11, 16, and 18 Immune Responses Generated by the Quadrivalent HPV Vaccine

    DEFF Research Database (Denmark)

    Nygård, Mari; Saah, Alfred; Munk, Christian;

    2015-01-01

    This quadrivalent human papillomavirus (qHPV) (HPV6, -11, -16, and -18) vaccine long-term follow-up (LTFU) study is an ongoing extension of a pivotal clinical study (FUTURE II) taking place in the Nordic region. The LTFU study was designed to evaluate the effectiveness, immunogenicity, and safety...... of the qHPV vaccine (Gardasil) for at least 10 years following completion of the base study. The current report presents immunogenicity data from testing samples of the year 5 LTFU visit (approximately 9 years after vaccination). FUTURE II vaccination arm subjects, who consented to being followed...... the proportion based on cLIA, especially for anti-HPV18. As expected, the anti-HPV serum IgG and cLIA responses were strongly correlated for all HPV types. Anti-HPV GMTs and the proportion of vaccinated individuals who are seropositive remain high for up to 9 years of follow-up after vaccination....

  6. 3,3'-Diindolylmethane induces anti-human gastric cancer cells by the miR-30e-ATG5 modulating autophagy.

    Science.gov (United States)

    Ye, Yang; Fang, Yanfei; Xu, Wenxia; Wang, Qiang; Zhou, Jianwei; Lu, Rongzhu

    2016-09-01

    3,3'-Diindolylmethane (DIM), a class of relatively non-toxic indole derivatives from cruciferous vegetables, has been reported as a promising anticancer phytochemical, but the underlying molecular mechanism is not completely elucidated. In the present study we report a novel regulation of autophagy by DIM in human gastric cancer cells. We found that DIM dose-dependently inhibited the growth of gastric cancer cells in vitro and in vivo. Moreover, ATG5 and LC3 were activated by DIM in gastric cancer cells. Furthermore, miR-30e was down-regulated by DIM and miR-30e targeted the 3'-UTR of ATG5 to inhibit its translation. Overall, these results suggest that DIM may through the miR-30e-ATG5 modulating autophagy inhibit the proliferation of gastric cancer cells. PMID:27372603

  7. Alpha-momorcharin (α-MMC) exerts effective anti-human breast tumor activities but has a narrow therapeutic window in vivo.

    Science.gov (United States)

    Cao, Dongliang; Sun, Yun; Wang, Ling; He, Qianchuan; Zheng, Juecun; Deng, Fei; Deng, Shanshan; Chang, ShuChing; Yu, XiaoPing; Li, Minhui; Meng, Yao; Jin, Jiagui; Shen, Fubing

    2015-01-01

    Alpha-momorcharin (α-MMC), a ribosome inactivating protein (RIP) extracted from the seeds of Momordica charantia, exerts anti-tumor, antiviral, and anti-fungal activities. However, α-MMC has an obvious toxicity that limits its clinical application. We examined the effect of α-MMC on the inhibition of human breast cancer and assessed its general toxicity to find the therapeutic window in vivo for its potential clinical use. It was purified using column chromatography, and then injected into the xenograft nude mouse model induced by MDA-MB-231 and MCF-7. The anti-tumor efficacy was evaluated with T/C%. Next, the α-MMC was injected at a series of doses to Balb/C mice to assess its general toxicity. The MTT assay, the apoptosis test, and the cell cycle inhibition of α-MMC in human breast cancer cells were performed. In the xenografted tumors induced by MDA-MB-231 and MCF-7, α-MMC exerted an obvious inhibition effects on tumor growth at the dosage of 1.2mg/kg and 0.8 mg/kg. For in vivo toxicity experiments of α-MMC in Balb/C mice, the minimal toxic dose of α-MMC was 1.2mg/kg. Alpha-MMC induced apoptosis by increasing caspase3 activities, and the cell cycle was arrested at the G0/G1 or G2/M phases. The measurements of IC50 were 15.07 μg/mL, 33.66 μg/mL, 42.94 μg/mL for MDA-MB-231, MCF-7 and MDA-MB-453 respectively. Alpha-MMC exhibits anti-tumor effects in human breast cancer in vivo and in vitro. It inhibits breast cancer cells through the inhibition of tumor growth and induction of cell apoptosis. However, due to its obvious toxicity, α-MMC has a relatively narrow therapeutic window in vivo. PMID:25447153

  8. In Vitro Preclinical Testing of Nonoxynol-9 as Potential Anti-Human Immunodeficiency Virus Microbicide: a Retrospective Analysis of Results from Five Laboratories

    OpenAIRE

    Beer, Brigitte E.; Doncel, Gustavo F.; Krebs, Fred C.; Shattock, Robin J; Fletcher, Patricia S.; Buckheit, Robert W; Watson, Karen; Dezzutti, Charlene S.; Cummins, James E.; Bromley, Ena; Richardson-Harman, Nicola; Pallansch, Luke A.; Lackman-Smith, Carol; Osterling, Clay; Mankowski, Marie

    2006-01-01

    The first product to be clinically evaluated as a microbicide contained the nonionic surfactant nonoxynol-9 (nonylphenoxypolyethoxyethanol; N-9). Many laboratories have used N-9 as a control compound for microbicide assays. However, no published comparisons of the results among laboratories or attempts to establish standardized protocols for preclinical testing of microbicides have been performed. In this study, we compared results from 127 N-9 toxicity and 72 efficacy assays that were genera...

  9. In vitro preclinical testing of nonoxynol-9 as potential anti-human immunodeficiency virus microbicide: a retrospective analysis of results from five laboratories.

    Science.gov (United States)

    Beer, Brigitte E; Doncel, Gustavo F; Krebs, Fred C; Shattock, Robin J; Fletcher, Patricia S; Buckheit, Robert W; Watson, Karen; Dezzutti, Charlene S; Cummins, James E; Bromley, Ena; Richardson-Harman, Nicola; Pallansch, Luke A; Lackman-Smith, Carol; Osterling, Clay; Mankowski, Marie; Miller, Shendra R; Catalone, Bradley J; Welsh, Patricia A; Howett, Mary K; Wigdahl, Brian; Turpin, Jim A; Reichelderfer, Patricia

    2006-02-01

    The first product to be clinically evaluated as a microbicide contained the nonionic surfactant nonoxynol-9 (nonylphenoxypolyethoxyethanol; N-9). Many laboratories have used N-9 as a control compound for microbicide assays. However, no published comparisons of the results among laboratories or attempts to establish standardized protocols for preclinical testing of microbicides have been performed. In this study, we compared results from 127 N-9 toxicity and 72 efficacy assays that were generated in five different laboratories over the last six years and were performed with 14 different cell lines or tissues. Intra-assay reproducibility was measured at two-, three-, and fivefold differences using standard deviations. Interassay reproducibility was assessed using general linear models, and interaction between variables was studied using step-wise regression. The intra-assay reproducibility within the same N-9 concentration, cell type, assay duration, and laboratory was consistent at the twofold level of standard deviations. For interassay reproducibility, cell line, duration of assay, and N-9 concentration were all significant sources of variability (P concentrations for N-9 were similar between laboratories for assays of similar exposure durations, but these similarities decreased with lower test concentrations of N-9. Results for both long (>24 h) and short (levels that were obtained by different laboratories using various protocols. This comparative work can be used to develop standardized microbicide testing protocols that will help advance potential microbicides to clinical trials. PMID:16436731

  10. In vivo anticancer evaluation of the hyperthermic efficacy of anti-human epidermal growth factor receptor-targeted PEG-based nanocarrier containing magnetic nanoparticles

    Directory of Open Access Journals (Sweden)

    Baldi G

    2014-06-01

    Full Text Available Giovanni Baldi,1 Costanza Ravagli,1 Filippo Mazzantini,1 George Loudos,2 Jaume Adan,3 Marc Masa,3 Dimitrios Psimadas,2 Eirini A Fragogeorgi,2 Erica Locatelli,4 Claudia Innocenti,5,6 Claudio Sangregorio,5,7 Mauro Comes Franchini4 1CERICOL, Sovigliana-Vinci, Italy; 2Technological Educational Institute of Athens, Athens, Greece; 3Leitat Technological Center, Barcelona, Spain; 4Department of Industrial Chemistry Toso Montanari, University of Bologna, Bologna, 5Consorzio Interuniversitario Nazionale per la Scienza e Tecnologia dei Materiali (INSTM, 6Dipartimento di Chimica U Schiff, Università di Firenze, Firenze, 7Centro Nazionale delle Ricerche (ICCOM – CNR, Firenze, Italy Abstract: Polymeric nanoparticles with targeting moieties containing magnetic nanoparticles as theranostic agents have considerable potential for the treatment of cancer. Here we report the chemical synthesis and characterization of a poly(D,L-lactide-co-glycolide-b-poly(ethylene glycol-based nanocarrier containing iron oxide nanoparticles and human epithelial growth factor receptor on the outer shell. The nanocarrier was also radiolabeled with 99mTc and tested as a theranostic nanomedicine, ie, it was investigated for both its diagnostic ability in vivo and its therapeutic hyperthermic effects in a standard A431 human tumor cell line. Following radiolabeling with 99mTc, the biodistribution and therapeutic hyperthermic effects of the nanosystem were studied noninvasively in vivo in tumor-bearing mice. A substantial decrease in tumor size correlated with an increase in both nanoparticle concentration and local temperature was achieved, confirming the possibility of using this multifunctional nanosystem as a therapeutic tool for epidermoid carcinoma. Keywords: magnetic nanoparticles, polymeric nanocarriers, skin cancer, hyperthermia, single-photon emission computed tomography, imaging

  11. Murine leukemia virus-based Tat-inducible long terminal repeat replacement vectors: a new system for anti-human immunodeficiency virus gene therapy.

    OpenAIRE

    Cannon, P M; Kim, N.; Kingsman, S M; Kingsman, A J

    1996-01-01

    We have constructed new murine leukemia virus (MLV)-based vectors (TIN vectors) which, following integration, contain human immunodeficiency virus (HIV) type 1 U3 and R sequences in place of the MLV U3 and R regions. This provides, for the first time, single transcriptional unit retroviral vectors under the control of Tat. TIN vectors have several advantages for anti-HIV gene therapy applications.

  12. Effect of an anti-human Co-029/tspan8/Tspan8 mouse monoclonal antibody on tumour growth in a nude mouse model

    Directory of Open Access Journals (Sweden)

    Naouel eAilane

    2014-09-01

    Full Text Available New therapeutic agents are needed in digestive tract tumours. Co-029/tspan8 is a tetraspanin frequently expressed on human colorectal tumours, In this work, we report the effects of the monoclonal antibody Ts29.2, targeting Co-029/tspan8, on colorectal tumor cells in vitro and after implantation in nude mice. HT29, Isreco1 and SW480 colorectal tumor cell lines were used for this study. HT29 has a strong endogenous expression of Co-029/tspan8, whereas Isreco1 cells don’t express Co-029/tspan8 and SW480 has only a weak expression. Isreco1 and SW480 were transduced to express Co-029/tspan8 at the same level as HT29. In order to check the specificity of the effect of monoclonal antibody Ts29.2, low Co029/tspan8 expressing SW480 cells were injected simultaneously with transduced cells in the back, on the left and right sides of the mice. With an early treatment, Ts29.2 mAb inhibited growth of tumors expressing Co-029/tspan8 up to 70%, whereas a delayed treatment was less efficient. No effect of the antibody on cell proliferation or apoptosis induction was detected in vitro. No increase of activated caspase 3 labeling was observed in vivo and areas occupied by vessels were not significantly different between treated mice and controls. This suggests that the action of Ts29.2 is linked neither to cellular toxicity nor to the inhibition of the previously reported angiogenic properties of Co-029/tspan8. An inhibition of cell proliferation in vivo is demonstrated by a reduction of the mitotic index in HT29 tumors of Ts29.2 treated mice. The discrepancy between in vitro and in vivo data on cell proliferation suggests that the binding of Ts29.2 to tumour cells may modify their response to signals issued from the microenvironment. Given the restricted pattern of tissue expression of the tetraspanin Co-029/tspan8, these preliminary results put forth for consideration the antibody targeting of this tetraspanin in further investigations for therapeutic applications.

  13. [Potency determination of horse anti-human-lymphocyte serum (ALS) by means of the lymphocyte resistance test, a modification of Schroder's leukocyte resistance test].

    Science.gov (United States)

    Mundt, B; Preussner, S; Frick, G; Bast, G

    1975-01-01

    The leukocyte resistance test of Schröder was used for the purpose of modifying the in vitro determination of cytotoxic potency of ALS during the course of immunization. In contrast to the original method, where the test was carried out with whole blood, the incubation was made with pure lymphocyte cell suspensions. The results obtained show that the extensive procedure of examination enables the cytotoxic effectiveness of antilymphocytic antibodies to be evaluated. PMID:54295

  14. ImmunoGlobulin galaxy (IGGalaxy) for simple determination and quantitation of immunoglobulin heavy chain rearrangements from NGS

    NARCIS (Netherlands)

    M.J. Moorhouse (Michael); D. van Zessen (David); H. IJspeert (Hanna); S. Hiltemann (Saskia); S. Horsman (Sebastiaan); P.J. van der Spek (Peter); M. van der Burg (Mirjam); A. Stubbs (Andrew)

    2014-01-01

    textabstractBackground: Sequence analysis of immunoglobulin heavy chain (IGH) gene rearrangements and frequency analysis is a powerful tool for studying the immune repertoire, immune responses and immune dysregulation in health and disease. The challenge is to provide user friendly, secure and repro

  15. Proteomic and genetic analysis of wheat endosperm albumins and globulins using deletion lines of cultivar Chinese Spring

    DEFF Research Database (Denmark)

    Merlino, Marielle; Bousbata, Sabrina; Svensson, Birte;

    2012-01-01

    identified using mass spectrometry and data mining. Salt-soluble endosperm proteins from 67 CS deletion lines were also separated by 2DE (four gels per line). Image analysis of the 268 2DE gels as compared to the CS reference proteome allowed the detection of qualitative and quantitative variations in...... endosperm proteins due to chromosomal deletions. This differential analysis of spots allowed structural or regulatory genes, encoding 211 proteins, to be located on segments of the 21 wheat chromosomes. In addition, variance analysis of quantitative variations in spot volume showed that the expression of...

  16. Thrombocytopenia associated with dengue hemorrhagic fever responds to intravenous administration of anti-D (Rh(0)-D) immune globulin.

    Science.gov (United States)

    de Castro, Reynaldo Angelo C; de Castro, Jo-Anne A; Barez, Marie Yvette C; Frias, Melchor V; Dixit, Jitendra; Genereux, Maurice

    2007-04-01

    Severe thrombocytopenia and increased vascular permeability are two major characteristics of dengue hemorrhagic fever (DHF). An immune mechanism of thrombocytopenia due to increased platelet destruction appears to be operative in patients with DHF (see Saito et al., 2004, Clin Exp Immunol 138: 299-303; Mitrakul, 1979, Am J Trop Med Hyg 26: 975-984; and Boonpucknavig, 1979, Am J Trop Med Hyg 28: 881-884). The interim data of two randomized placebo controlled trials in patients (N = 47) meeting WHO criteria for dengue hemorrhagic fever (DHF) with severe thrombocytopenia (platelets WinRho SDF), 50 microg/kg (250 IU/kg) intravenously is more brisk than the placebo group. The mean maximum platelet count of the anti-D-treated group at 48 hours was 91,500/mm(3) compared with 69,333/mm(3) in the placebo group. 75% of the anti-D-treated group demonstrated an increase of platelet counts > or = 20,000 compared with only 58% in the placebo group. These data suggest that treatment of severe thrombocytopenia accompanying DHF with anti-D may be a useful and safe therapeutic option. PMID:17426181

  17. Pathogen safety of intravenous Rh immunoglobulin liquid and other immune globulin products: enhanced nanofiltration and manufacturing process overview.

    Science.gov (United States)

    Soluk, Lori; Price, Hugh; Sinclair, Chris; Atalla-Mikhail, Dina; Genereux, Maurice

    2008-01-01

    Plasma products for therapeutic use pose specific challenges in manufacturing to ensure products maintain biologic activity and are safe with respect to contamination and transmission of disease-causing agents. Various processes have demonstrated effectiveness in eliminating, reducing, or inactivating viral contaminants. Recently, the possibility of transmitting variant Creutzfeld-Jakob disease (vCJD) or transmissible spongiform encephalopathies (TSE) through blood-based products has become a concern. The present study involves the validation of a hyperimmune immunoglobulin manufacturing process incorporating a nanofiltration step with a nominal pore size of 20 nm for removal of viral contaminants and other adventitious agents. Processing intermediates during the manufacture of IV Rh IgG (WinRho SDF/WinRho SDF Liquid, Cangene Corporation, Manitoba, Canada) were spiked with model viruses and processed in scaled-down procedures to validate the viral reduction capacity of each step. Anion-exchange chromatography and solvent/detergent steps are known to contribute to virus removal and inactivation. The Planova 20 N nanofiltration step was effective in reducing model viruses representing a wide range of viral morphologies with varying degrees of resistance to physicochemical inactivation. All in-process and final batch testing met current standards for production of IV Rh IgG manufactured with the previously licensed filter, which had a larger nominal pore size of 35 nm. The manufacturing process, employing a Planova 35 N filtration step, has been proactively improved by the change to a smaller-pore 20 N filter. Replacement of the 35 N filter with the 20 N filter produces a similar product while enhancing the capability for removal of smaller viruses and prions. PMID:18806519

  18. Competitive ligand - binding assay for thyroxine binding globulin. Comparison with TBG radioimmunoassay and T3 uptake test

    International Nuclear Information System (INIS)

    A simple and reproducible competitive ligand binding assay has been utilized to measure serum TBG concentration. In euthyroid subjects TBG concentration (mean +- SD, mg/l) was 33.7 +- 4; hyperthyroid 24 -+ 6; T3-thyrotoxicosis 20 +- 7; hypothyroid 37 -+ 7; pregnant 67 -+ 18; post-partum period 59.8 -+ 17; oral contraceptives 45 -+ 7. The correlation of CLBA with RIA measurement of TBG was significant (p3 uptake test (p4: TBG ratio according to serum T4 and TBG concentration provided a reliable index in the assessment of thyroid function

  19. Changes in Testosterone Levels and Sex Hormone-Binding Globulin Levels in Extremely Obese Men after Bariatric Surgery

    Science.gov (United States)

    Laichuthai, Nitchakarn; Suwannasrisuk, Preaw; Houngngam, Natnicha; Udomsawaengsup, Suthep; Snabboon, Thiti

    2016-01-01

    Objective. Obesity is a risk factor for hypogonadotropic hypogonadism in men. Weight loss has been shown to improve hypogonadism in obese men. This study evaluated the early changes in sex hormones profile after bariatric surgery. Methods. This is a prospective study including 29 morbidly obese men. Main outcomes were changes in serum levels of total testosterone (TT), free testosterone (cFT), SHBG, estradiol, adiponectin, and leptin at 1 and 6 months after surgery. Results. The mean age of patients was 31 ± 8 years and the mean BMI was 56.8 ± 11.7 kg/m2. Fifteen patients underwent Roux-en-Y gastric bypass and 14 patients underwent sleeve gastrectomy. At baseline, 22 patients (75.9%) had either low TT levels (<10.4 nmol/L) or low cFT levels (<225 pmol/L). Total testosterone and SHBG levels increased significantly at 1 month after surgery (p ≤ 0.001). At 6 months after surgery, TT and cFT increased significantly (p ≤ 0.001) and 22 patients (75.9%) had normalized TT and cFT levels. There were no changes in estradiol levels at either 1 month or 6 months after surgery. Conclusions. Increases in TT and SHBG levels occurred early at 1 month after bariatric surgery while improvements in cFT levels were observed at 6 months after bariatric surgery.

  20. Reduced Intensity Conditioning With Clofarabine, Antithymocyte Globulin (ATG), Total Lymphoid Irradiation (TLI) Followed by Allogeneic Stem Cell Transplant

    Science.gov (United States)

    2016-01-21

    Acute Myeloid Leukemia; Myelodysplastic Syndrome; Acute Lymphocytic Leukemia; Relapsed/Refractory Chronic Lymphocytic Leukemia; Relapsed/Refractory Non Hodgkin's Lymphoma; Hodgkins Disease; Relapsed Refractory Multiple Myeloma

  1. Regional distribution and variation of gamma-globulin absorption from the small intestine of the neonatal calf

    International Nuclear Information System (INIS)

    125I-labeled immunoglobulin (Ig)G1 in colostral whey was used to determine the region of maximum absorption of Ig from the small intestine of the neonatal calf and the variation in Ig absorption among calves at the intestinal level. In experiment 1, 5 segments (approx 5%, 35%, 60%, 80%, and 95% of the duodenocecal length) were formed in the small intestine of 9 colostrum-deprived calves shortly after birth. These segments were injected with colostral whey containing 125I-IgG1 4 hours after birth, and uptake, transfer, and absorption (defined as uptake plus transfer) were determined for each segment 2 hours later. Raw data were adjusted for the milligrams of IgG1 injected per gram of intestinal tissue to obtain the least squares mean (LSM) value. The LSM values for absorption of IgG1 from distal segments 3, 4, and 5 were significantly greater (P less than 0.05) than those values for proximal segments 1 and 2. The region of the maximum IgG1 absorption was the lower small intestine, 60% to 80% of the duodenocecal length. There was also an indication of independence between uptake and transfer in each of the segments. Significant differences (P less than 0.05) were present among calves in the LSM values for uptake and absorption, but not for transfer. In experiment 2, thoracic ducts of 8 newborn calves were cannulated 4 to 5 hours after birth. At 6 hours after birth, colostral whey with 125I-IgG1 was injected into an intestinal segment (approx 60% to 80% of the duodenocecal length)

  2. Myasthenia gravis treated with purified antithymocyte antiserum.

    Science.gov (United States)

    Pirofsky, B; Reid, R H; Bardana, E J; Baker, R L

    1979-01-01

    The therapeutic effect of goat anti-human thymocyte antiserum globulin (ATG) was assessed in 10 patients with myasthenia gravis. All subjects had far-advanced, debilitating disease poorly responsive to anticholinesterase therapy. Prolonged, low-dose ATG therapy was used, with 1.0 to 2.6 gm ATG protein administered intramuscularly over a 28- to 73-day period. Therapeutic responses of varying degrees were noted in 8 of 10 patients. Completion of a course of ATG treatment and discontinuation of the drug did not lead to acute relapse. Follow-up examinations for over 5 years have been maintained. A mean remission period of approximately 2 years was observed. This therapy deserves further evaluation; subjects with progressive myasthenia gravis despite prior thymectomy may represent ideal candidates. PMID:311448

  3. Testosterone-estradiol-binding globulin in patients with Turner's syndrome: effects of estrogens and acute growth hormone administration

    Energy Technology Data Exchange (ETDEWEB)

    Bergink, E.W.; Wolf, D.L.; Wittliff, J.L.; Bruining, G.J.; Bryson, M.F.; Forbes, G.B.

    1976-06-01

    The TeBG activity of plasma from patients with Turner's syndrome was measured quantitatively using polyacrylamide gel electrophoresis. Human growth hormone administration did not significantly change the plasma TeBG levels. However, oral replacement therapy with estrogens elevated plasma TeBG within 3 days; after 9 days these levels reached a maximum of three- to four-fold greater than that observed at a time prior to therapy.

  4. Identification of Albizia lebbeck seed coat chitin-binding vicilins (7S globulins) with high toxicity to the larvae of the bruchid Callosobruchus maculatus

    Energy Technology Data Exchange (ETDEWEB)

    Souza, A.J. [Laboratório de Química e Função de Proteínas e Peptídeos, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense, Campos dos Goytacazes, RJ (Brazil); Ferreira, A.T.S.; Perales, J.; Beghini, D.G. [Laboratório de Toxinologia, Departamento de Fisiologia e Farmacodinâmica, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ (Brazil); Fernandes, K.V.S.; Xavier-Filho, J.; Venancio, T.M.; Oliveira, A.E.A. [Laboratório de Química e Função de Proteínas e Peptídeos, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense, Campos dos Goytacazes, RJ (Brazil)

    2011-01-27

    Seed coat is a specialized maternal tissue that interfaces the embryo and the external environment during embryogenesis, dormancy and germination. In addition, it is the first defensive barrier against penetration by pathogens and herbivores. Here we show that Albizia lebbeck seed coat dramatically compromises the oviposition, eclosion and development of the bruchid Callosobruchus maculatus. Dietary supplementation of bruchid larvae with A. lebbeck seed coat flour causes severe weight loss and reduces survival. By means of protein purification, mass spectrometry and bioinformatic analyses, we show that chitinbinding vicilins are the main source of A. lebbeck tegumental toxicity to C. maculatus. At concentrations as low as 0.1%, A. lebbeck vicilins reduce larval mass from 8.1 ± 1.7 (mass of control larvae) to 1.8 ± 0.5 mg, which corresponds to a decrease of 78%. Seed coat toxicity constitutes an efficient defense mechanism, hindering insect predation and preventing embryo damage. We hypothesize that A. lebbeck vicilins are good candidates for the genetic transformation of crop legumes to enhance resistance to bruchid predation.

  5. Associations of sex-hormone-binding globulin (SHBG) with non-SHBG-bound levels of testosterone and estradiol in independently living men

    NARCIS (Netherlands)

    de Ronde, W; van der Schouw, YT; Muller, M; Grobbee, DE; Gooren, LJG; Pols, HAP; de Jong, FH

    2005-01-01

    Results of in vitro experiments indicate that with increasing concentrations of SHBG, testosterone ( T) is preferentially bound to SHBG in comparison with estradiol (E2). In these studies, the ratio of non-SHBG-bound E2 (non-SHBG-E2) to non-SHBG-T increased with increasing levels of SHBG. SHBG has c

  6. Identification of Albizia lebbeck seed coat chitin-binding vicilins (7S globulins with high toxicity to the larvae of the bruchid Callosobruchus maculatus

    Directory of Open Access Journals (Sweden)

    A.J. Souza

    2012-02-01

    Full Text Available Seed coat is a specialized maternal tissue that interfaces the embryo and the external environment during embryogenesis, dormancy and germination. In addition, it is the first defensive barrier against penetration by pathogens and herbivores. Here we show that Albizia lebbeck seed coat dramatically compromises the oviposition, eclosion and development of the bruchid Callosobruchus maculatus. Dietary supplementation of bruchid larvae with A. lebbeck seed coat flour causes severe weight loss and reduces survival. By means of protein purification, mass spectrometry and bioinformatic analyses, we show that chitin-binding vicilins are the main source of A. lebbeck tegumental toxicity to C. maculatus. At concentrations as low as 0.1%, A. lebbeck vicilins reduce larval mass from 8.1 ± 1.7 (mass of control larvae to 1.8 ± 0.5 mg, which corresponds to a decrease of 78%. Seed coat toxicity constitutes an efficient defense mechanism, hindering insect predation and preventing embryo damage. We hypothesize that A. lebbeck vicilins are good candidates for the genetic transformation of crop legumes to enhance resistance to bruchid predation.

  7. Inhibin A, inhibin B, follicle-stimulating hormone, luteinizing hormone, estradiol, and sex hormone-binding globulin levels in 473 healthy infant girls

    DEFF Research Database (Denmark)

    Chellakooty, M; Schmidt, I M; Haavisto, A M;

    2003-01-01

    The early postnatal regulation of reproductive hormones seems to be more complex in girls than in boys. The aim of this study was to describe inhibins A and B, FSH, LH, estradiol, and SHBG in a large prospective cohort of 473 unselected, healthy, 3-month-old girls. In full term, appropriate...

  8. Effect of Maillard reaction on biochemical properties of peanut 7S globulin (Ara h 1) and its interaction with a human colon cancer cell line (Caco-2)

    NARCIS (Netherlands)

    Teodorowicz, M.; Fiedorowicz, E.; Kostyra, H.; Wichers, H.J.; Kostyra, E.

    2013-01-01

    Purpose The purpose of this study was to determine the influence of Maillard reaction (MR, glycation) on biochemical and biological properties of the major peanut allergen Ara h 1. Methods Three different time/temperature conditions of treatment were applied (37, 60, and 145 °C). The extent of MR wa

  9. Effect of Maillard reaction on biochemical properties of peanut 7S globulin (Ara h 1) and its interaction with a human colon cancer cell line (Caco-2)

    OpenAIRE

    Teodorowicz, M.; Fiedorowicz, E.; Kostyra, H.; Wichers, H J; Kostyra, E.

    2013-01-01

    Purpose The purpose of this study was to determine the influence of Maillard reaction (MR, glycation) on biochemical and biological properties of the major peanut allergen Ara h 1. Methods Three different time/temperature conditions of treatment were applied (37, 60, and 145 °C). The extent of MR was assessed by SDS-PAGE, loss of free amino groups, fluorescence intensity, content of bound sugar and fructosamine. The Caco-2 model system was applied to study effects of hydrolysed and non-hydrol...

  10. Fludarabine Phosphate, Busulfan, and Anti-Thymocyte Globulin Followed By Donor Peripheral Blood Stem Cell Transplant, Tacrolimus, and Methotrexate in Treating Patients With Myeloid Malignancies

    Science.gov (United States)

    2016-05-04

    Accelerated Phase Chronic Myelogenous Leukemia; Adult Acute Myeloid Leukemia in Remission; Adult Acute Myeloid Leukemia With 11q23 (MLL) Abnormalities; Adult Acute Myeloid Leukemia With Del(5q); Adult Acute Myeloid Leukemia With Inv(16)(p13;q22); Adult Acute Myeloid Leukemia With t(15;17)(q22;q12); Adult Acute Myeloid Leukemia With t(16;16)(p13;q22); Adult Acute Myeloid Leukemia With t(8;21)(q22;q22); Blastic Phase Chronic Myelogenous Leukemia; Childhood Acute Myeloid Leukemia in Remission; Childhood Chronic Myelogenous Leukemia; Childhood Myelodysplastic Syndromes; Chronic Phase Chronic Myelogenous Leukemia; de Novo Myelodysplastic Syndromes; Hematopoietic/Lymphoid Cancer; Myelodysplastic/Myeloproliferative Neoplasm, Unclassifiable; Previously Treated Myelodysplastic Syndromes; Recurrent Adult Acute Myeloid Leukemia; Recurrent Childhood Acute Myeloid Leukemia; Relapsing Chronic Myelogenous Leukemia

  11. Intravenous immune globulin (10% caprylate-chromatography purified) for the treatment of chronic inflammatory demyelinating polyradiculoneuropathy (ICE study): a randomised placebo-controlled trial

    NARCIS (Netherlands)

    R.A.C. Hughes (Richard); P. Donofrio (Peter); V. Bril (Vera); M.C. Dalakas (Marinos); C. Deng (Chunqin); K. Hanna (Kim); H.P. Hartung; N. Latov (Norman); I.S.J. Merkies (Ingemar); P.A. van Doorn (Pieter)

    2008-01-01

    textabstractBackground: Short-term studies suggest that intravenous immunoglobulin might reduce disability caused by chronic inflammatory demyelinating polyradiculoneuropathy (CIDP) but long-term effects have not been shown. We aimed to establish whether 10% caprylate-chromatography purified immune

  12. Lower levels sex hormone-binding globulin independently associated with metabolic syndrome in pre-elderly and elderly men in China

    Institute of Scientific and Technical Information of China (English)

    Xiao-Na Pang; Yu Hu; Ying Yuan; Ji-Ping Shen; Xiao-Yun Zha; Xuan Sun

    2013-01-01

    pre-elderly and elderly men in China. Methods A cross-sectional study was done among 437 men, aged 45 to 94 years old. Early morning fasting sera were assayed for total testosterone (TT), SHBG and other biochemical markers. Free testosterone (FT) was calculated. Results The SHBG level of the MetS group was significantly lower than those without MetS 35.70 (25.18, 47.10) nmol/L vs. 41.90 (31.80, 55.20) nmol/L; P < 0.001). As the number of MetS components increases, SHBG and TT levels became lower. SHBG correlated with age, as did TT and most of metabolic components. Body mass index (BMI), high density lipoprotein-cholesterol (HDL-C), triglyceride (TG), and TT remained independently associated with SHBG by multivariate regression analysis. In a logistic regression taking MetS as the dependent variable, SHBG (95% confidence interval (95% CI): 0.975-0.994, P = 0.018) and homeostasis model assessment for insulin resistance (HOMA-IR) (95%CI: 1.535-2.647, P < 0.001) were included in the final model. Conclusions Lower SHBG is independently associated with MetS among pre-elderly and elderly men. SHBG may be an independent predictor of MetS, but the mechanism of how SHBG is involved in MetS requires further studied.

  13. Interaction of xenobiotics with estrogen receptors α and β and a putative plasma sex hormone-binding globulin from channel catfish (Ictalurus punctatus)

    Science.gov (United States)

    Gale, William L.; Patino, Reynaldo; Maule, Alec G.

    2004-01-01

    Estrogens are important regulators of physiological functions. Although environmental contaminants (xenoestrogens) which interfere with estrogen signaling are of increasing concern, there is only limited information about their ability to interact with estrogen-binding proteins (SHBG) or receptors (ER). Recombinant ER?? and ?? were obtained after transient transfection of COS-7 cells with channel catfish ER cDNA. Plasma from adult female channel catfish was the source of SHBG. Tritiated estradiol ( 3H-E2) was used in standard radioligand-binding assays to characterize the binding properties of channel catfish SHBG (ccfSHBG) and to estimate the inhibition constants for various estrogenic compounds. Binding of 3H-E2 to ccfSHBG was saturable and of high affinity with a Kd (??SE) of 1.9??0.14nM and a Bmax of 14.3??2.4pmol/mg protein (n=3 assays). Additionally, ccfSHBG displayed binding specificity for androgens and estrogens. Endosulfan, 4-nonylphenol, and 4-octylphenol displaced 3H-E2 binding to ccfSHBG albeit only at very high concentrations, whereas dieldrin and atrazine showed little displacement activity even at the highest concentrations used. The synthetic estrogen ethynylestradiol had higher affinity than E2 for ccfSHBG. This finding differs from results with human and rainbow trout SHBG. The alkylphenolic compounds (4-octylphenol and 4-nonylphenol) displayed some ability to displace 3H-E2 binding from ER?? and ?? at high concentrations, but dieldrin and atrazine had little binding activity for both ER subtypes and endosulfan for ER??. The xenobiotics tested generally showed equivalent or greater affinity for ER?? than ER??, whereas natural estrogens had much greater affinity for ER?? than ER??. These observations suggest that results of studies using fish tissue ER extracts must be interpreted with caution, since both ER subtypes may be present, and that the binding of xenoestrogens to SHBG must be taken into account for proper assessment of endocrine disruption caused by environmental contaminants.

  14. Inhibin A, inhibin B, follicle-stimulating hormone, luteinizing hormone, estradiol, and sex hormone-binding globulin levels in 473 healthy infant girls

    DEFF Research Database (Denmark)

    Chellakooty, M; Schmidt, I M; Haavisto, A M;

    2003-01-01

    The early postnatal regulation of reproductive hormones seems to be more complex in girls than in boys. The aim of this study was to describe inhibins A and B, FSH, LH, estradiol, and SHBG in a large prospective cohort of 473 unselected, healthy, 3-month-old girls. In full term, appropriate......-for- gestational-age girls (n = 355) hormones showed a marked interindividual variation, with concentrations up to pubertal values [medians (95% confidence intervals): inhibin B, 82 pg/ml (...

  15. The Potential Biochemical Diagnosis Criteria and Therapeutic Effect Indexes: Sex Hormone Binding Globulin Levels and Free Androgen Index in Blood of Patients With Polycystic Ovary Syndrome

    Science.gov (United States)

    2013-02-02

    The Investigators Collected 534 PCOS Patients as the Case Group,and 580 Infertile Women With Normal Ovulatory Cycle of the Control Group;; At the Same Time, the Investigators Continuedly Collect Cases to October 2012, and Totally Collected 579 Patients With PCOS Altogether;; 534 Patients in the Cases Group and 580 Women in the Control Group Received no Measures, While 579 Patients Received Drugs;; The Investigators Monitored Basic Indexes in Blood of All the Subjects in This Suvey,and Also Monitored Indexes of 579 Patients After Treatment.

  16. Vicilins (7S storage globulins of cowpea (Vigna unguiculata seeds bind to chitinous structures of the midgut of Callosobruchus maculatus (Coleoptera: Bruchidae larvae

    Directory of Open Access Journals (Sweden)

    Sales M.P.

    2001-01-01

    Full Text Available The presence of chitin in midgut structures of Callosobruchus maculatus larvae was shown by chemical and immunocytochemical methods. Detection by Western blotting of cowpea (Vigna unguiculata seed vicilins (7S storage proteins bound to these structures suggested that C. maculatus-susceptible vicilins presented less staining when compared to C. maculatus-resistant vicilins. Storage proteins present in the microvilli in the larval midgut of the bruchid were recognized by immunolabeling of vicilins in the appropriate sections with immunogold conjugates. These labeling sites coincided with the sites labeled by an anti-chitin antibody. These results, taken together with those previously published showing that the lower rates of hydrolysis of variant vicilins from C. maculatus-resistant seeds by the insect's midgut proteinases and those showing that vicilins bind to chitin matrices, may explain the detrimental effects of variant vicilins on the development of C. maculatus larvae.

  17. Gene duplication and an accelerated evolutionary rate in 11S globulin genes are associated with higher protein synthesis in dicots as compared to monocots

    OpenAIRE

    Li Chun; Li Meng; Dunwell Jim M; Zhang Yuan-Ming

    2012-01-01

    Abstract Background Seed storage proteins are a major source of dietary protein, and the content of such proteins determines both the quantity and quality of crop yield. Significantly, examination of the protein content in the seeds of crop plants shows a distinct difference between monocots and dicots. Thus, it is expected that there are different evolutionary patterns in the genes underlying protein synthesis in the seeds of these two groups of plants. Results Gene duplication, evolutionary...

  18. 人去唾液酸糖蛋白受体单克隆抗体制备及其初步应用%Preparation of mouse anti-human asialoglycoprotein receptor monoclonal antibody and its preliminary application

    Institute of Scientific and Technical Information of China (English)

    李军; 陈磊; 孙斌; 张妤; 钱海华; 殷正丰

    2013-01-01

    目的 制备人去唾液酸糖蛋白受体(ASGPR)的小鼠单克隆抗体,并应用该抗体检测ASGPR在细胞系和组织中的表达.方法 对ASGPR H1大亚基进行序列分析,选取ASGPR1全长序列制备免疫原.通过RT PCR合成ASGPR1cDNA,构建ASGPR1表达重组载体,在E.coli BL21中表达后纯化,获得目的抗原.应用传统融合杂交瘤技术制备小鼠单克隆抗体,常规检测单抗亚类和效价,竞争抑制实验鉴定单抗特异性.用流式细胞术和免疫组织化学法分别检测ASGPR在多种肝源性与非肝源性细胞系以及多种肝组织中的表达.结果 制备的单克隆抗体亚型为IgG1,效价达1∶12 800.竞争抑制实验结果显示该单抗具有很好的ASGPR结合特异性,而且识别的肽段位于ASGPR胞外段.流式细胞术检测结果显示,ASGPR不同程度地表达于肝源性细胞系,但不表达于非肝源性细胞系.免疫组织化学检测结果显示,ASGPR专一性表达于正常肝组织和肝癌组织,在肝癌组织的表达与分化程度有关,高分化肝癌中的阳性率高于低分化肝癌(75.0% vs 28.6%,P<0.05).结论 成功制备高特异性人ASGPR小鼠单克隆抗体,适用于用流式细胞术和免疫组织化学法检测ASGPR表达,可用于临床病理鉴定原发性肝癌与转移性肝癌.

  19. 人OX40单克隆抗体的制备、纯化及初步鉴定%The Preparation,Purification and Preliminary Identification of Anti-human OX40 Monoclonal Antibody

    Institute of Scientific and Technical Information of China (English)

    崔杰; 辛宁; 冯永堂; 鞠吉雨; 王成伟; 刘艳菲; 苗乃法

    2010-01-01

    目的 应用杂交瘤技术,制备鼠抗人OX40单克隆抗体.方法 以本室纯化的OX40胞外段融合蛋白免疫6~8周龄的雌性BalB/c小鼠,取免疫小鼠脾细胞与SP2/0骨髓瘤细胞融合,筛选分泌鼠抗人OX40单克隆抗体的杂交瘤细胞阳性克隆.用杂交瘤细胞接种小鼠腹腔诱生腹水,腹水经饱和硫酸铵纯化沉淀后用间接ELISA法测定抗体的Ig类别.SDS-PAGE分析抗体的纯化程度,用免疫组化技术鉴定单克隆抗体与表达有OX40分子的组织及细胞特异性结合的情况.结果 获得1株能稳定分泌鼠抗人OX40单克隆抗体的杂交瘤细胞,细胞株诱生的小鼠腹水纯化后抗体纯度较高,Ig类别为IgG,蛋白浓度为6.129g/L.制备的单抗经淋巴细胞免疫组化及炎性淋巴组织免疫组化技术初步鉴定结果表明,该抗体与OX40单克隆抗体标准品制剂相比具有类同的特异性.结论 成功获得能稳定分泌鼠抗人OX40单克隆抗体的杂交瘤细胞株,制备的单克隆抗体具有较高的纯度和活性.

  20. Anti-Human Immunodeficiency Virus Type 1 (HIV-1 ⅢB) Activity of Lectin from Sea Mussel Crenomytilus Grayanus%贻贝凝集素抗-HIV活性研究

    Institute of Scientific and Technical Information of China (English)

    李伟; 熊川男; 王建华; 郑永唐; 金桥; 佟长青; 曲敏; 刘洪伟; 汪秋宽

    2007-01-01

    分离提取海洋无脊椎动物贻贝(Crenomytilus grayanus)凝集素,考察其抗HIV活性.采用半乳糖-Sepharose 6B亲和层析和Sephacryl S-200层析分离提取贻贝凝集素(Crenomytilus grayanus lectin,CGL),以光镜检查合胞体抑制试验,以ELSA测定HIVp24抗原表达水平.从海洋无脊椎动物贻贝中分离出的凝集素(CGL),为N-乙酰半乳糖胺/半乳糖(GalNAc/Gal)特异性的凝集素.CGL在27.88mg·L-1浓度时,对HIV诱导细胞病变的抑制达50%;在45.70mg·L-1时,对HIV-1复制的抑制达50%;同时在35.12mg·L-1浓度时,对HIV感染细胞融合的阻断达50%.

  1. 中西医药抗宫颈人乳头瘤病毒感染的研究进展%Progress of Study on Anti-human Cervical Papilloma Virus Infection with Chinese and Western Medicine

    Institute of Scientific and Technical Information of China (English)

    李明成; 刘微; 邵为荣

    2007-01-01

    人乳头瘤病毒(human papilloma virus,HPV)感染与宫颈癌及其癌前病变发生密切相关,高危型HPV通过E6和E7基因表达相应蛋白诱发宫颈癌.宫颈HPV的早发现和早预防是阻断其癌变的关键.中西医药结合是预防HPV感染和阻止宫颈癌变的重要途径,从天然药物开发抗病毒化合物已成为研究热点.

  2. T cells and T-cell subsets in mycosis fungoides and parapsoriasis. A study of 18 cases with anti-human T-cell monoclonal antibodies and histochemical techniques.

    Science.gov (United States)

    Buechner, S A; Winkelmann, R K; Banks, P M

    1984-07-01

    Skin lesions from 15 patients with mycosis fungoides (MF) and from three with parapsoriasis were studied immunohistochemically with monoclonal antibodies against T cells (Leu 1) and against T-cell subsets (Leu 2a, Leu 3a). Lymphoid cell reactivity was diverse among these sampled cases. In two cases of parapsoriasis and nine of MF, there was a predominance of helper/inducer (Leu-3a-reactive) cells over suppressor/cytotoxic (Leu-2a-reactive) cells. In one case of parapsoriasis and one (advanced tumor stage) of MF, there was suppressor/cytotoxic cell predominance. One case of MF showed strong reactivity for both T-cell subset markers. Four cases of MF (two plaque-stage and two tumor-stage) featured a predominant cell type in the dermis which was nonreactive for all three antibodies. The intraepidermal lymphoid cellularity was Leu-1-reactive in ten cases of MF and two of parapsoriasis. Among these 12 cases, the intraepidermal cellularity was Leu-2a-reactive in four and Leu-3a-reactive in three. The use of such studies of T-cell subsets on in situ cutaneous lymphoid infiltrates may demonstrate a correlation with cytomorphology, clinical stage, and disease prognosis.

  3. Preparation and Purification of the Monoclonal Antibody for Anti-human Lung Squamous Carcinoma%抗人肺鳞癌单克隆抗体的制备和纯化

    Institute of Scientific and Technical Information of China (English)

    王红明; 唐睿珠; 马丽菊; 郝萍

    2013-01-01

    目的 制备并纯化前期工作建立的杂交瘤细胞株C5所分泌的抗人肺鳞癌单克隆抗体,为研究该单抗对应肺癌相关抗原的生物学功能奠定基础.方法 常规培养前期工作建立的C5杂交瘤细胞,有限稀释法进行克隆化培养,ELISA法检测培养上清,挑选检测阳性细胞继续克隆化培养,直至3次检测亚克隆均呈阳性.收集阳性培养上清,利用Protein A-sepharoseCL-4B亲和层析法纯化抗体,Western-Blot法对纯化后的抗体进行鉴定.结果 得到了来源相同的亚克隆杂交瘤细胞株,克隆阳性率达100%.成功纯化出了目的单克隆抗体,并证实纯化产物系目的单抗,纯化后的C5杂交瘤细胞单克隆抗体重链分子量约为75 kDa.结论 成功纯化出抗人肺鳞癌单克隆抗体,该单抗可能在肺癌的早期诊断、靶向治疗和肿瘤疫苗的研制等方面具有重要意义和潜在应用价值.%Objective To subclone C5 hybridoma cells and purify the monoclonal antibody (McAb) by the immunoaffinity chromatography so as to provide with purified McAb the future research,the early diagnosis of lung cancers and targeted therapy and to study the lung cancer associated with antigen and the antigen associated with vaccine.Method C5 hybridoma cells were thawed from liquid nitrogen.The culture of hybridoma cells was subcloned for 3 times,and the culture's supernatant from each clone was tested by ELISA assay.Positive clones underwent culture expansion and the McAb was purified from its supernatant using ProteinaA-SepharoseCL-4B column affinity chromatography procedure.The specificity of the McAb protein expression was measured using SDS-PAGE and Western-Blot assay.Results the McAb was purified from C5 lung cancer hybridoma cell line using affinity chromatography technique.SDS-PAGE and Western Blot assay of the McAb showed one single band was specifically against the second antibody and the molecular weight of which was about 75KDa.Conclusion The McAb against lung cancer cells can be successfully purified from C5 hybridoma cells by limited dilution and affinity chromatography using ProteinaA-SepharoseCL-4B column.This McAb may play an important role in the early diagnosis and targeted therapy of human lung cancer and may contribute to the development of tumor vaccine.

  4. GMP production and characterization of the bivalent anti-human T cell immunotoxin, A-dmDT390-bisFv(UCHT1) for phase I/II clinical trials.

    Science.gov (United States)

    Woo, Jung Hee; Liu, Jen-Sing; Kang, Soo Hyun; Singh, Ravibhushan; Park, Seong Kyu; Su, Yunpeng; Ortiz, Janelle; Neville, David M; Willingham, Mark C; Frankel, Arthur E

    2008-03-01

    The bivalent anti-T cell immunotoxin, A-dmDT390-bisFv(UCHT1), was developed for treatment of T-cell leukemia, autoimmune diseases and tolerance induction for transplantation. To obtain clinical grade bivalent anti-T cell immunotoxin for phase I/II clinical trials, a single batch of 120 L bioreactor culture was performed using the Pichia pastoris mutEF2JC307-8(2) strain expressing the bivalent anti-T cell immunotoxin. After 162 h induction of the culture by methanol, the culture medium was harvested by a 0.1 microm hollow-fiber microfiltration step. The recombinant protein was purified by a 3-step purification procedure (Butyl 650 M capturing step, borate anion exchange step and final Poros anion exchange step). The final material was filter sterilized, aseptically vialed, and stored at -80 degrees C. Expression level was 207 mg/L of culture supernatant and the final production yield was 69.6% or 144.2mg/L of culture supernatant. The final product was characterized by multiple assays. Vialed product was sterile. The drug concentration was 0.8 mg/mL in 150 mM NaCl, 5% glycerol, 1mM EDTA, and 5mM Tris (pH 8.0). Purity by SDS-PAGE was 98%. Aggregates by Superdex 200 HPLC were toxin fusion drugs for clinical development. PMID:18160309

  5. Anti-human CD73 monoclonal antibody inhibits metastasis formation in human breast cancer by inducing clustering and internalization of CD73 expressed on the surface of cancer cells

    DEFF Research Database (Denmark)

    Terp, Mikkel G; Olesen, Kristina A; Christensen, Eva Arnspang;

    2013-01-01

    -linking of CD73, because both whole IgG anti-CD73 AD2 mAb and Fab' fragments thereof exhibited this effect. Ex vivo treatment of different breast cancer cell lines with anti-CD73 AD2 mAb before i.v. injection into mice inhibited extravasation/colonization of circulating tumor cells and significantly reduced...

  6. Anti-human α-synuclein N-terminal peptide antibody protects against dopaminergic cell death and ameliorates behavioral deficits in an AAV-α-synuclein rat model of Parkinson's disease.

    Directory of Open Access Journals (Sweden)

    Md Shahaduzzaman

    Full Text Available The protein α-synuclein (α-Syn has a central role in the pathogenesis of Parkinson's disease (PD and immunotherapeutic approaches targeting this molecule have shown promising results. In this study, novel antibodies were generated against specific peptides from full length human α-Syn and evaluated for effectiveness in ameliorating α-Syn-induced cell death and behavioral deficits in an AAV-α-Syn expressing rat model of PD. Fisher 344 rats were injected with rAAV vector into the right substantia nigra (SN, while control rats received an AAV vector expressing green fluorescent protein (GFP. Beginning one week after injection of the AAV-α-Syn vectors, rats were treated intraperitoneally with either control IgG or antibodies against the N-terminal (AB1, or central region (AB2 of α-Syn. An unbiased stereological estimation of TH+, NeuN+, and OX6 (MHC-II immunostaining revealed that the α-Syn peptide antibodies (AB1 and AB2 significantly inhibited α-Syn-induced dopaminergic cell (DA and NeuN+ cell loss (one-way ANOVA (F (3, 30 = 5.8, p = 0.002 and (F (3, 29 = 7.92, p = 0.002 respectively, as well as decreasing the number of activated microglia in the ipsilateral SN (one-way ANOVA F = 14.09; p = 0.0003. Antibody treated animals also had lower levels of α-Syn in the ipsilateral SN (one-way ANOVA F (7, 37 = 9.786; p = 0.0001 and demonstrated a partial intermediate improvement of the behavioral deficits. Our data suggest that, in particular, an α-Syn peptide antibody against the N-terminal region of the protein can protect against DA neuron loss and, to some extent behavioral deficits. As such, these results may be a potential therapeutic strategy for halting the progression of PD.

  7. 抗人AEG-1单克隆抗体可变区基因克隆及序列分析%Developing an Anti-human AEG-1 Monoclonal Antibody Secreting Hybridoma and Its Variable Region Fragment Amplification

    Institute of Scientific and Technical Information of China (English)

    龙敏; 董柯; 王希; 陈曦; 刘冲; 刘丽; 张惠中

    2014-01-01

    AEG-1基因位于人染色体8q22,编码582个氨基酸,参与多种信号转导途径并与多种恶性肿瘤的发生、发展及生物学表型密切相关.为更好地探讨AEG-1生物学功能,以纯化的pGSTag-AEG-1蛋白免疫BALB/c小鼠,应用细胞融合技术并经筛选及鉴定,获得了分泌抗人AEG-1单克隆抗体的杂交瘤细胞株1E3;Western blot及免疫组化证实该细胞株分泌的单克隆抗体能与肿瘤细胞中AEG-1蛋白特异性结合;RT-PCR方法从1E3细胞中克隆出抗AEG-1抗体的VH和VL基因片段,通过测序分析、碱基和蛋白序列的比对确认该株抗体为鼠源性IgG的轻、重链可变区基因.进一步运用Kabat System在线分析系统对VH和VL基因进行结构分析,确证FWRs和CDRs的结构完整,VH编码117个氨基酸;VL编码119个氨基酸,属于轻链κV家族.实验结果为进一步研究AEG-1与恶性肿瘤发生、发展的关系及在其临床诊断中的应用奠定了基础.

  8. The 1.7 A X-ray crystal structure of the porcine factor VIII C2 domain and binding analysis to anti-human C2 domain antibodies and phospholipid surfaces.

    Directory of Open Access Journals (Sweden)

    Caileen M Brison

    Full Text Available The factor VIII C2 domain is essential for binding to activated platelet surfaces as well as the cofactor activity of factor VIII in blood coagulation. Inhibitory antibodies against the C2 domain commonly develop following factor VIII replacement therapy for hemophilia A patients, or they may spontaneously arise in cases of acquired hemophilia. Porcine factor VIII is an effective therapeutic for hemophilia patients with inhibitor due to its low cross-reactivity; however, the molecular basis for this behavior is poorly understood. In this study, the X-ray crystal structure of the porcine factor VIII C2 domain was determined, and superposition of the human and porcine C2 domains demonstrates that most surface-exposed differences cluster on the face harboring the "non-classical" antibody epitopes. Furthermore, antibody-binding results illustrate that the "classical" 3E6 antibody can bind both the human and porcine C2 domains, although the inhibitory titer to human factor VIII is 41 Bethesda Units (BU/mg IgG versus 0.8 BU/mg IgG to porcine factor VIII, while the non-classical G99 antibody does not bind to the porcine C2 domain nor inhibit porcine factor VIII activity. Further structural analysis of differences between the electrostatic surface potentials suggest that the C2 domain binds to the negatively charged phospholipid surfaces of activated platelets primarily through the 3E6 epitope region. In contrast, the G99 face, which contains residue 2227, should be distal to the membrane surface. Phospholipid binding assays indicate that both porcine and human factor VIII C2 domains bind with comparable affinities, and the human K2227A and K2227E mutants bind to phospholipid surfaces with similar affinities as well. Lastly, the G99 IgG bound to PS-immobilized factor VIII C2 domain with an apparent dissociation constant of 15.5 nM, whereas 3E6 antibody binding to PS-bound C2 domain was not observed.

  9. A Quantitative Measurement of Antiviral Activity of Anti-Human Immunodeficiency Virus Type 1 Drugs against Simian Immunodeficiency Virus Infection: Dose-Response Curve Slope Strongly Influences Class-Specific Inhibitory Potential

    OpenAIRE

    Deng, Kai; Zink, M. Christine; Clements, Janice E; Siliciano, Robert F.

    2012-01-01

    Simian immunodeficiency virus (SIV) infection in macaques is so far the best animal model for human immunodeficiency virus type 1 (HIV-1) studies, but suppressing viral replication in infected animals remains challenging. Using a novel single-round infectivity assay, we quantitated the antiviral activities of antiretroviral drugs against SIV. Our results emphasize the importance of the dose-response curve slope in determining the inhibitory potential of antiretroviral drugs and provide useful...

  10. 试论中国反贩运人口法律制度的完善%The Incentives,Defects and Countermeasures of Anti-Human Trafficking in China' s Legal System

    Institute of Scientific and Technical Information of China (English)

    刘国福

    2010-01-01

    贩运人口是一种现代形式的奴役,严重侵害被贩运者的人权,危害社会和谐稳定.虽然有证据证明,中国的贩运人口情况很严重和需要加大对反贩运人口的法学研究,但是如何发展反贩运人口法律,仍然缺少系统的分析和严谨的论述.面对严峻的贩运人口犯罪形势,有必要完善反贩运人口法律制度,为打击贩运人口提供更为良好的法治环境.

  11. Protein electrophoresis - serum

    Science.gov (United States)

    ... of protein and fat, called lipoproteins (such as LDL cholesterol). ... globulin proteins may indicate: Abnormally low level of LDL cholesterol Malnutrition Increased gamma globulin proteins may indicate: Bone ...

  12. Effect of superposition and masking between red blood cell autoantibodies and alloantibodies.

    Science.gov (United States)

    Yu, Y; Wang, D Q

    2014-01-01

    This study aimed to explore the law of superposition and masking between autoantibodies and alloantibodies, and to ensure the detection of alloantibodies and to improve the safety of warm autoimmune hemolytic anemia patients. Eight kinds of commercial IgG red blood cell antibody reagents were serially diluted, and 3 kinds of antibodies at dilutions showing a continuous gradual decline in agglutination strength with the corresponding antigen red blood cells were treated as the target antibodies. Anti-D and anti-M were treated as simulated autoantibodies, and anti-Fya was treated as a simulated alloantibody. Four concentrations, 4+, 3+, 2+ and 1+, of autoantibodies and three concentrations, 3+, 2+ and 1+, of alloantibodies were combined, and 12 kinds of hybrid antibodies were detected and evaluated by the anti-human globulin micro-column gel assay. When the simulated strong autoantibody (4+) was used, the alloantibodies (3+, 2+, 1+) had no effect on the final agglutination strength; when the strength of agglutination produced by the simulated autoantibody was less than 4+, and at the same time there were alloantibodies (3+, 2+, 1+), the differences in agglutination strength with a panel of RBCs could be clearly observed. Strong autoantibodies (4+) can exert a masking effect, leading to alloantibodies being undetected; autoantibodies less than 4+, will produce the superimposed effect with alloantibodies, resulting in differences in agglutination strength. PMID:25036516

  13. Blocked D phenomenon and relevance of maternal serologic testing.

    Science.gov (United States)

    Jain, Ashish; Kumawat, Vijay; Marwaha, Neelam

    2015-01-01

    A blood requisition for double-volume exchange transfusion was received for a 2-day-old male child born to a 29-year-old multiparous female (P2002) referred to our institute having neonatal jaundice with encephalopathy; no maternal sample was received. the neonatal blood sample was typed as group A, D-, and the direct antiglobulin test (DAT) was strongly positive (4+) using the gel method. Mono-specific DAT showed the presence of IgG antibodies on neonatal red blood cells (RBCs). Acid elution and gentle heat elution (at 56°C) confirmed the presence of anti-D on neonatal RBCs. The baby received two exchange transfusions with group O, D-, packed RBCs compatible with his own serum. Later, on day 3, the neonate's mother was typed as group AB, D-, and her serum revealed the presence of alloanti-D, -C, and -S reactive in the anti-human globulin phase. The anti-D titer was 1024. this report highlights the "blocking" phenomenon caused by maternal anti-D in a case of hemolytic disease of fetus and newborn with a positive DAT. PMID:26829177

  14. Fluorescence resonance energy transfer based immunosensing of human IgG by using quantum dot/GIgG-gold nanoparticles/IgG conjugation.

    Science.gov (United States)

    Luo, Lin; Liu, Zhao; Li, Jianjun; Zhu, Jian

    2014-06-01

    A novel immunosensor of human immune globulin (IgG) was fabricated based on the fluorescence transfer between luminescent semiconductor quantum dots (QDs) and gold nanoparticles (AuNPs). AuNPs and CdSe/ZnS QDs were respectively labeled with immune reaction pair:IgG and goat anti-human immunoglobulin (GIgG), by optimizing the conditions including pH value and protein amount. In the assembled QD-GIgG-IgG-AuNP fluorescence resonance energy transfer (FRET) immunocomplex system, the presence of AuNP-IgG directly reduced the fluorescence intensity of the GIgG conjugated QDs. As a result, the concentration of AuNP-IgG had a linear relationship with the fluorescence decrease in a range of 0-1.57 microg/mL. Furthermore, the mechanism of the QDs' fluorescence decay has also been discussed and attributed to the light-induced photobleaching. This novel sensing method achieves quantitative detection of trace proteins, suggesting the potential of biomolecule-AuNPs conjugation based analytical methods in further application. PMID:24738348

  15. A nylon ball solid-phase radioimmunoassay for specific antibodies in human sera. Application to measurement of IgG antibodies to pollen allergens

    International Nuclear Information System (INIS)

    The principle of the radioallergosorbent test (RAST) has been used to measure IgG antibodies to timothy grass pollen allergens in sera from desensitized allergic subjects. 125I-labeled goat anti-human IgG was used as detector protein. Non-specific binding was eliminated by use of a non-porous nylon ball an antigen carrier and by use of a special buffer with high ionic strength and pH, containing 1% bovine gamma globulin and 5% normal rabbit serum as 'balance proteins'. At dilution 1:80 non-specific binding was only 0.28% and the binding ratio for a high-titer serum was about 10. By inhibition experiments the assay was demonstrated to be specific for IgG antibodies to timothy grass pollen. The results obtained with this assay correlated statistically significantly with those found with a double-antibody method. Serum dilution curves were parallel, indicating that the assay is in allergen excess. The within-assay coefficient of variation ranged from 3.9 to 7.6%; the between-assay coefficient of variation from 8.4 to 19.5%. The assay is very simple to perform, requiring no centrifugation. The allergen-coated balls are stable for at least 3 months. The assay should be applicable to measurement of IgG antibodies and IgG subclass antibodies to any protein antigen of interest. (Auth.)

  16. 大豆11S和7S球蛋白对Alcalase凝固大豆蛋白性质的影响%Effects of 11S and 7S Globulins on the Clotting Properties of Soybean Protein by Alcalase

    Institute of Scientific and Technical Information of China (English)

    栾广忠; 程永强; 鲁战会; 李里特; 辰巳英三

    2005-01-01

    通过对不同比例的11S与7S大豆蛋白在被碱性蛋白酶(Alcalalse)凝固过程中分子量和TPA参数的测定,表明在Alcalase凝固大豆蛋白过程中,11S球蛋白是形成凝胶的主要物质.

  17. Effects of the Addition of L-Dipalmitoyl Phosphatidylcholine and -globulin on the Lubricating Behavior of Hyaluronic Acid%添加L€朌PPC和€柷虻鞍对透明质酸润滑性的影响

    Institute of Scientific and Technical Information of China (English)

    虞路清; 鲁毅强; 李久青; 张蓓

    2001-01-01

    A compound artificial synovia was prepared by adding L€杁ipalmitoyl phosphatidylcholine(L€朌PPC) riposomes and €杇lobulin to a saline of sodium hyaluronate (HA),by which two kind of tribology slider pairs€朠VA€朒/PVA€朒 and PVA€朒/316L S.S. were lubricated ,an oscillator€杢ype tri€朾iometer and M2000 wear test machine were used to determine their tribology parameters respectively. The experimental results showed that the lubricating behavior of compound synovia has a significantly improvement compared to the hyaluronic acid solution alone. It's probably that these improvements benefit from the adsorbed films on the PVA€朒 surface composed of protein and phospholipids and the boundary lubricating ability of the additives.%在透明质酸为主要成分的人工关节润滑剂中添加L€朌PPC磷脂和€柷虻鞍祝渲瞥筛春先斯せ海赑VA€朒/PVA€朒, PVA€朒/316L S.S. 2类摩擦副上,利用振子式摩擦仪和M2000型磨损实验机,测定其摩擦学参数.结果表明,与纯粹透明质酸溶液相比,复合人工滑液的润滑性能有显著提高.其润滑机制研究表明,润滑性能的提高可能得益于添加物质在PVA€朒材料表面所形成的磷脂双层膜及其良好的边界润滑能力.

  18. The Clinical Value Between Blood Serum Globulin/Cholinestevase and Prognosis of Liver Cirhosis%血清球蛋白/胆碱脂酶比值的临床价值

    Institute of Scientific and Technical Information of China (English)

    周国华; 陈叶青; 周红宇; 王为; 冷明芳; 王丽姣

    2007-01-01

    目的 探讨血清球蛋白/胆碱酯酶的比值的临床价值.方法 检测30例肝硬化患者和26例正常对照组G/C比值,并与白蛋白、胆红素、凝血酶原时间作对比分析.结果 (1)肝硬化患者血清球蛋白、胆碱酯酶均与CTP分级有相关性.(2)肝硬化患者血清G/C比值与血清白蛋白呈负相关,G/C比值与血清胆红素呈正相关,G/C比值与Pr呈正相关,且G/C比值、血清球蛋白、血清胆碱酯酶三者相比,差异均具有非常显著性(T=7.1709,P<0.05).结论 与血清胆红素、PT及胆碱酯酶相比,肝硬化患者血清G/C比值能更好地反映肝功能损害程度,对肝硬化的病情变化及预后判断具有重要的临床价值.

  19. Investigation of Genetic Variants, Birthweight and Hypothalamic-Pituitary-Adrenal Axis Function Suggests a Genetic Variant in the SERPINA6 Gene Is Associated with Corticosteroid Binding Globulin in the Western Australia Pregnancy Cohort (Raine) Study

    OpenAIRE

    Anderson, Laura N.; Laurent Briollais; Atkinson, Helen C.; Julie A Marsh; Jingxiong Xu; Connor, Kristin L.; Matthews, Stephen G.; Pennell, Craig E.; Stephen J Lye

    2014-01-01

    Background The hypothalamic-pituitary-adrenal (HPA) axis regulates stress responses and HPA dysfunction has been associated with several chronic diseases. Low birthweight may be associated with HPA dysfunction in later life, yet human studies are inconclusive. The primary study aim was to identify genetic variants associated with HPA axis function. A secondary aim was to evaluate if these variants modify the association between birthweight and HPA axis function in adolescents. Methods Morning...

  20. Variation in levels of serum inhibin B, testosterone, estradiol, luteinizing hormone, follicle-stimulating hormone, and sex hormone-binding globulin in monthly samples from healthy men during a 17-month period

    DEFF Research Database (Denmark)

    Andersson, Anna-Maria; Carlsen, Elisabeth; Petersen, Jørgen Holm;

    2003-01-01

    seasonal variation was observed in LH and testosterone levels, but not in the levels of the other hormones. The seasonal variation in testosterone levels could be explained by the variation in LH levels. The seasonal variation in LH levels seemed to be related to the mean air temperature during the month...

  1. Combined N-glycome and N-glycoproteome analysis of the Lotus japonicus seed globulin fraction shows conservation of protein structure and glycosylation in legumes

    DEFF Research Database (Denmark)

    Dam, Svend Secher; Thaysen-Andersen, Morten; Stenkjær, Eva;

    2013-01-01

    β-1,2-xylose and α-1,3-fucose. Two abundant Lotus seed N-glycoproteins were site-specifically profiled; a predicted lectin containing two fully occupied N-glycosylation sites carried predominantly pauci-mannosidic structures in different distributions. In contrast, Lotus convicilin storage protein 2...

  2. 野生大豆种子cDNA文库构建及其球蛋白基因克隆%Construction of cDNA Library and Cloning of Globulin Gene from Wild Soybean( Glycine soja ) Seeds

    Institute of Scientific and Technical Information of China (English)

    王清连; 石明旺

    2006-01-01

    以优质野生大豆(Glycine soja)的近成熟种子为材料成功构建了野生大豆cDNA文库,库容量为9.594×105克隆,文库的重组率约为93.7%.PCR检测重组克隆的插入片段平均大于1000bp,表明构建的近成熟种子cDNA文库质量较高.从文库中随机挑选252个克隆进行3'端测序,得到217条表达序列标签(EST),测序结果表明,片断大于500bp;野生大豆与栽培大豆(Glycine max)球蛋白的cDNA序列存在99%相似性.

  3. An electrophoretic characterization of serum proteins of the collared peccary (Tayassu tajacu).

    Science.gov (United States)

    Lochmiller, R L; Hellgren, E C; Varner, L W; Grant, W E

    1984-01-01

    Serum proteins of the collared peccary (Tayassu tajacu) were analyzed by agarose gel electrophoresis for wild adult males and females, nursing young, and reproductively-active females in captivity. Electrophoretic profiles of the adult peccary showed at least six distinct protein bands corresponding to the fractions: albumin, alpha-1, alpha-2, beta-1, beta-2, and gamma globulin. Globulin fractions of the peccary had different mobilities from the domestic swine. The only sexual dimorphisms were associated with the beta globulin:albumin ratio and the albumin:globulin ratio. Ingestion of colostrum in 1-day-old neonates was marked by a very large increase in gamma globulins. The only significant difference between pregnant and lactating females was in the alpha globulin:beta globulin ratio. Lactating females had higher concentrations of alpha-2 globulin than non-pregnant females.

  4. Reference serum protein and lipoprotein fractions of ostriches (Struthio camelus in Turkey : research communication

    Directory of Open Access Journals (Sweden)

    U. Polat

    2004-11-01

    Full Text Available The aim of this study was to determine for reference purposes the values of serum albumin, a1-globulin, a2-globulin, b-globulin, g-globulin, and a-lipoprotein (high density lipoprotein, pre-b-lipoprotein (very low density lipoprotein and b-lipoprotein (low density lipoprotein fractions of normal ostriches (Struthio camelus in Turkey. Five male and five female ostriches, 18 months old, were used. All the ostriches were fed on a diet that contained 15.14 % crude protein and 2 950 Kcal/kg of metabolizable energy. The serum protein and lipoprotein fractions were measured using agarose gel electrophoresis. The fractions were found to be 60.96 % albumin, 0.24% a1-globulin, 15.91 % a2-globulin, 13.34 % b-globulin, 9.55 % g-globulin, 53.77 % HDL, 0.60 % VLDL and 48.09 % LDL.

  5. Construction and Biological Activity Detection of an Anti-Human Red Blood Cell Single Chain Fragment Variable-Pseudorabies Virus gE Bifunctional Fusion Protein%抗人红细胞单链抗体(ScFv)-伪狂犬病毒(PRV)gE蛋白双功能融合蛋白的构建及生物学活性检测

    Institute of Scientific and Technical Information of China (English)

    廖文军; 覃绍敏; 吴健敏; 王仰杰; 白安斌; 覃建飞

    2010-01-01

    利用特异性引物从重组质粒pMD-2E8ScFv和pMD-gE中PCR扩增出2E8基因(抗人红细胞H抗原单链抗体基因)和kgE基因(PRVgE主要抗原编码区),采用重叠延伸(SOE)PCR法拼接成融合的双功能分子2E8kgE,经BamH I和EcoR I双酶切,纯化后,克隆至BamH I和EcoR I双酶切的表达载体pET-Trx中,构建了原核表达载体pET-Trx-2E8kgE;将pET-Trx-2E8kgE转化至BL21plysS中,在IPTG诱导下表达具有双功能的融合蛋白,经过SDS-PAGE和Western-blot检测;结果表明,重组菌BL21plysS表达出约60.1 kD的目的蛋白,与猪伪狂犬标准阳性血清发生特异性反应.红细胞凝集试验证实,2E8kgE融合蛋白既能够与人红细胞结合,又能够与PRV抗体反应,具有双功能特性.

  6. Construction, expression and identification of anti-human AFP VH single domain antibody fusion protein gene%抗AFP重链可变区单域抗体融合蛋白的构建、表达及初步鉴定

    Institute of Scientific and Technical Information of China (English)

    方丽; 王振达; 张平; 赵宗蓉; 章崇杰

    2007-01-01

    目的 构建抗甲胎蛋白(AFP)重链可变区(VH)单域抗体融合蛋白基因在大肠杆菌中的表达,并初步鉴定表达产物的活性.方法 从已构建的抗AFP单链抗体(ScFv)的载体中,经PCR扩增出VH 单域抗体基因,再克隆到融合蛋白表达载体pET32a(+)中进行表达;用SDS-PAGE及Western-blot鉴定表达产物;并通过竞争抑制ELISA分析TrxA-VH融合蛋白的结合活性;细胞免疫组化染色分析其内化及结合情况.结果 VH 单域抗体基因全长339 bp,将其克隆到pET32a(+)中,转化大肠杆菌可获得高效表达,Western-blot证实在相应分子质量处,有TrxA-VH融合蛋白的显色条带.经初步纯化和复性后,获得抗AFP的VH单域抗体融合蛋白.竞争抑制ELISA及细胞免疫组化证明,表达产物具有与AFP特异结合的活性.结论 成功构建了抗AFP的VH单域抗体融合蛋白,为临床的应用研究奠定了基础.

  7. 肾移植患者微小病毒B19抗体的检测和临床意义%Detection of anti-human parvovirus B19 antibodies in recipients after renal transplantation and its clinical significance

    Institute of Scientific and Technical Information of China (English)

    姚磊; 高蕾; 董解菊; 张艮甫

    2003-01-01

    人类微小病毒B19(Human parvovirus,PV-B19)是小DNA病毒属中惟一对人类致病的病毒,可引起传染性红斑、纯红系再障、先天性畸形等多种疾病。在免疫抑制患者中,PV-B19感染可导致多种疾病的发生。本研究应用ELISA方法检测了38例肾移植术后患者PV-B19抗体,并对部分肾移植术后患者进行了不同时相点的检测,现报告如下。

  8. 一株抗人OX40激发型单克隆抗体的研制及其生物学功能的初步研究%Preparation of anti-human OX40 agonist monoclonal antibody and its biological function

    Institute of Scientific and Technical Information of China (English)

    毛辉; 谢芳; 陈永井; 王勤; 张学光

    2010-01-01

    鼠抗人OX40分子激发型单克隆抗体(mAb)的研制及其生物学特性的鉴定.以高表达人OX40分子的基因转染细胞L929-OX40为免疫原,常规免疫6~8周龄的雌性BALB/c小鼠;采用B淋巴细胞融合技术,将小鼠脾脏细胞与骨髓瘤细胞SP2/0融合,并以L929-OX40细胞为阳性筛选细胞,L929-Moek细胞为阴性筛选细胞,采用免疫荧光技术对杂交瘤进行反复筛选及多次克隆化培养;采用快速定性试纸法检测抗体亚型;竞争抑制实验分析抗原结合表位;Western blot检测抗体特异性;采用MTT法分析单抗在体外对T细胞增殖的促进作用,并采用ELISA检测培养上清中的细胞因子.结果:获得一株稳定、高效分泌鼠抗人OX40单克隆抗体的杂交瘤细胞株(克隆号:9H8),该株mAb能够特异性识别U937、Jurkat、Thp-1等血液来源肿瘤细胞株表面OX40分子,并且能够有效促进T细胞活化、增殖及细胞因子的分泌.成功研制了一株分泌鼠抗人OX40分子激发型单克隆抗体的杂交瘤细胞株,该抗体能够特异性识别人OX40分子并能够介导OX40正性协同刺激效应.

  9. 鼠抗人OX40功能性单克隆抗体的研制及其生物学特性的鉴定%Preparation of anti-human OX40 functional monoclonal antibody and study of its biological characteristics

    Institute of Scientific and Technical Information of China (English)

    孙建军; 王勤; 陈永井; 居颂文; 邱玉华; 施勤; 于葛华; 时文彪; 张学光

    2005-01-01

    目的:研制功能性鼠抗人OX40单克隆抗体.方法:以转人OX40的转基因细胞L929-OX40为免疫原,常规免疫6~8周龄的雌性BALB/c小鼠;采用B淋巴细胞融合技术,将免疫小鼠脾脏细胞与SP2/0融合,以L929-OX40转基因细胞及PHA活化的T细胞为抗体筛选阳性细胞,经免疫荧光标记分析对杂交瘤进行反复筛选和多次的克隆化培养;采用快速定性试纸法及竞争抑制结合试验分析了该单抗的亚类及抗原识别位点;采用MTT法分析单抗在体外对T细胞的促增殖效应以及ELISA分析活化T细胞分泌的细胞因子.结果:获得1株持续、稳定分泌鼠抗人OX40单克隆抗体的杂交瘤细胞株(命名为7E11),该单抗能特异性地识别人OX40分子和介导有效的共刺激信号,体外促进活化的T细胞增殖和细胞因子的分泌.结论:成功研制成一株能分泌功能性鼠抗人OX40单克隆抗体的杂交瘤,该抗体特异性地识别人OX40分子并具有在体外协同刺激T细胞的作用.

  10. Inhibition of mouse acrosome reaction and sperm-zona pellucida binding by anti-human sperm membrane protein 1 antibody%抗人精子膜蛋白抗体1对小鼠精子顶体反应和精子-透明带结合的抑制作用

    Institute of Scientific and Technical Information of China (English)

    G.Y.Cheng; J.L.Shi; M.Wang; Y.Q.Hu; C.M.Liu; Y.F.Wang; C.Xu

    2007-01-01

    Aim:To investigate the possible functions of human sperm membrane protein (hSMP-1) in the process of fertilization.Methods: A 576-bp cDNA fragment of HSD-1 gene coding for the extracellular domain of hSMP-1 was cloned and expressed. The localization of this protein on human and mouse sperm was determined by indirect immunofluorescent staining by using anti-recombinant hSMP-1 (anti-rhSMP-1) antibodies. Sperm acrosome reaction and spermzona pellucida (ZP) binding assay were carried out in 10-week-old BALB/c mice. Results: Recombinant hSMP-1 was successfully cloned and expressed. The expression of the native protein was limited on the acrosome of human and mouse sperm. Treatment of anti-rhSMP-1 antibodies significantly decreased the average number of sperms bound to each egg. Meanwhile, the percentage of acrosome reaction was decreased in comparison to pre-immune control after treatment with anti-rhSMP-1 (P < 0.05). Conclusion: The results suggest that anti-rhSMP-1 antibody inhibited mouse acrosome reaction and sperm-ZP binding.

  11. 葡萄球菌肠毒素激活肿瘤浸润淋巴细胞及外周血淋巴细胞体外抗人卵巢癌的对比研究%Comparative study of anti-human ovarian cancer on TIL and PBL stimulated by SEA in vitro

    Institute of Scientific and Technical Information of China (English)

    李辞妹; 赵建武; 梁文通

    2012-01-01

    Objective:To investigate the anti-tumor activity of tumor-infiltrating lymphocytes ( TIL) and peripheral blood lymphocytes ( PBL) of ovarian cancer stimulated by staphylococcal enterotoxin A (SEA) in vitro. Methods: TIL were isolated from-tumor tissues and ascites, and PBL were isolated from the peripheral blood of 10 ovarian cancer patients with ascites. The isolated cells were then cultivated in the presence of SEA and IL-2. The proliferation of TIL and PBL were observed in vitro. Flow cytometer was used to detect the expression of CD3, CD4 and CD8 on the surface of TIL. The cytotoxic activity to K562 cells and autogenous tumor cells was evaluated by MTT colorimetric method and the concentration of TNF-A alpha--y in cultivating supernatant was measured by Enzyme-linked immunosorbent test(ELISA). Results ;TIL of ovarian solid carcinoma and TIL,PBL of ascites were successfully separated from 8 patients of 10 patients. (1) SEA had great stimulatory effect,which was higher than the group of IL-2 (P<0. 05) ,on the TIL of ovarian solid carcinoma, the TIL of ascites and PBL. After the peak of proliferation, it showed a downward trend, which was not seen in the group of IL-2. (2 )The expression rate of CD3+CD4+T-cell and CD3+CD8*T-cell was increased significantly. There was significant difference in the increasing rate of SEA group and IL-2 group (P<0. 05 ) , the rate of CD3+CD8+T which induced by SEA proliferated more quickly. (3) The killing activity of TIL on autologous tumor cells was much higher than that on K562 cells (P<0.05). However , the killing activity of PBL on autologous tumor cells was much lower than that on K562 cells (P<0.05). Each effector cells were stimulated by SEA had higher killing activity than that stimulated by IL-2 (P<0. 05). (4) TNF-alpha or IFN-gamma secreted by each effector cells reached peak on the second and the fouth day, and then the peak decreased rapidly. The level of TNF-alpha and IFN-gamma in the SEA group was higher than those in the IL-2 group ( P < 0.05). Conclusion; SEA can induce the antitumor acitivity of TIL efficiently and quickly.%目的:探讨葡萄球菌肠毒素A(SEA)对卵巢癌肿瘤浸润淋巴细胞(TIL)及其外周血淋巴细胞(PBL)抗瘤活性的诱导作用.方法:取10例卵巢癌伴腹水患者实体瘤、腹水及外周血标本,分离TIL和PBL.在SEA及IL-2作用下培养,定时计数,了解其增殖情况;流式细胞仪检测其CD3、CD4、CD8表达;噻唑蓝(MTT)比色法测定其对K562及自体肿瘤细胞的细胞毒活性;酶联免疫吸附试验(ELISA)测定培养上清液中TNF-a和IFN-γ浓度.结果:10例中8例成功分离实体瘤TIL、腹水TIL及PBL.(1)SEA刺激的实体瘤TIL、腹水TIL及PBL增殖速率明显较IL-2诱导组快(P<0.05),但增殖高峰后出现下降趋势,IL-2组未出现此现象;(2) CD3+ CD4+及CD3+CD8+T表达率均明显上升,其中SEA诱导组比IL-2组增加比例明显(P<0.05),以SEA作用的CD3+ CD8+T比例增加最快;(3)TIL对自体肿瘤细胞的杀伤活性明显高于对K562细胞的杀伤活性(P<0.05),PBL对自体肿瘤细胞的杀伤活性则明显低于对K562细胞的杀伤活性(P<0.05),SEA激活组比IL-2组杀伤率高(P<0.05);(4)各效应细胞分泌的TNF-a、IFN-γ分别在培养的第2天和第4天达到高峰,高峰后迅速下降,SEA诱导组在前10天明显高于IL-2诱导组(P<0.05).结论:SEA可高效、迅速诱导卵巢癌TIL的抗瘤活性.

  12. Anti-human immunodeficiency virus type 1 activity of two new nonnucleoside reverse transcriptase inhibitors in combination with other 3 antiretroviral agents in vitro%两种非核苷类逆转录酶抑制剂与已上市药物联合应用的体外抗HIV活性研究

    Institute of Scientific and Technical Information of China (English)

    庄道民; 刘思扬; 董如华; 白丽; 姜标; 赵小龙; 李敬云

    2009-01-01

    目的:评价两种非核苷类逆转录酶抑制剂(JB25、JB26)与3种已上市药物齐多夫定(AZT)、依非韦仑(EFV)、沙奎那韦(SQV)联合应用的体外抗HIV活性.方法:将10个浓度的JB25、JB26分别与AZT、EFV、SQV的7个浓度组成各种浓度的组合,加入384孔细胞培养板,与HIV-1 ⅢB急性感染的MT-2细胞共培养3 d,最后利用TZM bl细胞报告基因检测HIV-1的表达,共重复3次.利用MacSynergy Ⅱ软件对数据进行分析,得到药物相互作用的情况.结果:JB25与AZT、EFV、SQV的平均协同/拮抗容量分别是244.45/-5.05, 119.58/-65.93,145.83/-0.32(nmol/L)~2%;JB26与AZT、EFV、SQV的平均协同/拮抗容量分别是398.90/0, 103.62/-0.49, 138.473/-0.27(nmol/L)~2%.结论:两种非核苷类逆转录酶抑制剂与3种已上市的药物在体外联合应用,具有协同抗HIV作用,MacSynergy Ⅱ软件可以全面评价两种药物的联合作用情况.

  13. Preparation and sequence analysis of Vk gene of anti-human bFGF McAb%抗bFGF McAb制备、鉴定及轻链可变区基因克隆和序列分析

    Institute of Scientific and Technical Information of China (English)

    崔蕴霞; 王一飞; 林剑; 任哲; 银巍; 宁波; 冯炼强

    2000-01-01

    目的:以重组人碱性成纤维细胞生长因子为抗原免疫小鼠,建立多株稳定分泌bFGF单克隆抗体(McAb)的杂交瘤细胞.并对bFGF单克隆抗体(McAb)可变区基因进行扩增及序列分析 .方法:采用Western blot法和免疫沉淀法对抗体特异性和Ig类型进行鉴定,同时,应用分子生物学技术,对Vk基因的DNA片段进行克隆,并对4a2Vk基因进行序列测定.结果:bFGF单克隆抗体可特异性结合人重组bFGF抗原,且均为IgM.由杂交瘤4a2、3d3细胞株扩增Vk基因是由330个碱基组成,编码110个氨基酸残基.通过国际联机检索进行EMBL和Kabat基因库扫描发现,4a2 Vk DNA仅与Ig同源,符合小鼠Ig的Vk基因特征;同源性为90%~98%,应归属于Ig的Vk基因.结论:杂交瘤4a2、3d3细胞株可稳定分泌bFGF单克隆抗体,并获得其轻链可变区基因,为进一步构建和表达单链Fv(ScFv)抗体打下良好的基础.

  14. 抗人表皮生长因子受体2亲和体 ZHER2瞷342的18F 标记及靶向胃癌的示踪研究%Tracing investigation of targeting gastric cancer with 18F labeled anti-human epidermal growth factor receptor 2 specific affibody molecule ZHER2:342

    Institute of Scientific and Technical Information of China (English)

    潘云云; 柏志成; 潘栋辉; 徐宇平; 杨润林; 王立振; 管文贤; 杨敏

    2016-01-01

    目的:探讨18 F标记抗人表皮生长因子受体2( HER2)亲和体探针在HER2过表达胃癌中的示踪作用。方法化学合成制备C末端含半胱氨酸的抗HER2亲和体 ZHER2瞷342,通过双功能螯合剂1,4,7-三氮杂环壬烷-1,4,7三乙酸的马来酰亚胺衍生物( NOTA-MAL)与巯基的加成反应合成NOTA-MAL-Cys59-ZHER2:342偶联物(简称偶联物),经18 FAl一步法定位标记制得新型HER2靶向分子探针18FAl-NOTA-MAL-Cys59ZHER2瞷342(简称探针),并经高效液相色谱法(HPLC)进行质量控制。构建NOD SCID鼠HER2高表达胃癌NCI N87移植瘤模型。进行体外细胞摄取、阻断、竞争结合实验和模型鼠micro PET显像以评价探针的靶向能力。结果18 F标记探针放化纯度>95%。细胞结合实验显示HER2过表达NCI N87细胞对18 F标记亲和体的摄取速度快,孵育15 min后接近摄取高峰,约(7.48±0.49)%ID。阻断HER2后,细胞对标记物的摄取水平显著下降,15 min 为(0.85±0.09)%ID (P<0.05),说明NCI N87对亲和体的摄取是通过HER2所特异性介导的。细胞竞争结合实验测得IC50为9.4 nM,说明探针与HER2结合亲和力高。 NOD SCID鼠micro PET显像肿瘤摄取高,30 min为(7.22±0.24)%ID/g。阻断组micro PET显像肿瘤摄取显著降低,1h为(2.56±0.11)%ID/g(P<0.05)。探针主要经肾脏排泄。结论新型探针标记方便,对HER2过表达胃癌靶向能力强。%Objective To investigate the effect of 18 F labeled anti-HER2 affibody probe on the targeting HER2 overexpressed human gastric cancer with micro PET imaging . Methods Anti-HER2 specific affibody ZHER2:342 was obtained from chemical synthesis routes .The bifunctional maleimide derivative of 1,4,7-Triazacyclononane-1,4,7-triacetic acid (NOTA) was coupled to thiol-group of cysteine of ZHER2:342 to form the chelator-peptide conjugation .Then the newly produced 18 F was labeled to the conjugation through 18FAl one step labeling.The resulting radiolabeled product was analyzed by high performance liquid chromatography (HPLC).The characteristics of 18FAl-NOTA-MAL-Cys59 ZHER2:342 binding to NCI N87 were assessed by using the in vitro cell uptaking and blocking assays , competition binding assay.NOD SCID mice models bearing HER 2 positive human gastric cancer tumor was established . Micro PET imaging was conducted to evaluate the targeting ability of the probe with xenograft models . Results The radiochemical purity of 18 F labeled ZHER2:342 was more than 95%.The NCI N87 cell associated radioactivity was close to the platform , approximately(7.48 ±0.49)%ID, as early as 15 minute after incubation together .Blocking analysis demonstrated that the binding of 18 F-ZHER2:342 to living HER2-overexpressed cells was receptor mediated .Competition binding assay with NCI N 87 indicated IC50 was 9.4nM.The microPET imaging of human gastric cancer xenograft demonstrated that the radioactive probe could specifically accumulate in the tumor tissue of (7.22 ±0.24)%ID/g 30 min after injection.The quantitive date didn ’ t decrease significantly with time extending .The uptaking level decreased apparently after blocking HER2. Conclusions The novel 18 F labeled ZHER2:342 could be acquired easily and effectively targeting gastric cancer with HER 2 overexpression .

  15. 中国反贩运人口法律的理性回顾和发展思考:以国际法为视角%Review and Thinking on China's Anti-Human-Trafficking Law: A Perspective of the International Law

    Institute of Scientific and Technical Information of China (English)

    刘国福

    2010-01-01

    不论是中国政府,还是美国、救助儿童会等其他国家和非政府组织都认为中国贩运人口非常严重.贩运人口侵害了被贩运者的人权.对贩运人口的需求是产生贩运人口现象的主要原因,不完善的法律制度在抑制需求和截断供给方面的消极作用不容忽视.以国际法视角梳理中国反贩运人口法律的历史发展,剖析其与加入的国际法律文件的差距,提出反贩运人口的法律对策,对于打击贩运人口,构建与中国应当履行的国家义务相一致的反贩运人口法律制度有着重要的意义.

  16. Type and screen policy in the blood bank: Is AHG cross-match still required? A study at a multispecialty corporate hospital in India

    Directory of Open Access Journals (Sweden)

    Pathak Sangeeta

    2011-01-01

    Full Text Available Background: Antibodies against only about 25-28 blood group antigens are known to cause hemolytic reactions (HTRs, and red cell antibody screening should detect such clinically significant antibodies. An extension of the antibody screening test is the ′type and screen′ done to detect clinically significant antibodies, omiting the anti-human globulin (AHG cross-match. Aim: The aim of this study was to find out if the type and screen procedure is a safe method for pre-transfusion testing when compared to the AHG cross-match currently in use in India. Materials and Methods: We evaluated data from 45373 patients for whom a total of 61668 units of packed red blood cells (PRBC were cross-matched in the AHG phase using DiaMed; ID cards. An antibody screen was carried out in all the patients using the DiaMed; ID-DiaCell I+II+III. The AHG cross-match was also carried out for all recipients, irrespective of the result of the antibody screen. The results were compared to see if there were any cases where the antibody screening was negative but the AHG cross-match showed incompatibility. Results: Not a single case was found where the antibody screen was negative and AHG cross-match showed incompatibility. In 68 cases the antibody screening was positive. Out of the 68 cases, AHG cross-match was incompatible with at least one unit of PRBC in 41 cases. Conclusion: The screening cell panel adequately detected the clinically significant antibodies in the Indian population in our study. The type and screen policy can be safe, efficient, cost-effective, and beneficial to the transfusion service in India.

  17. Radioimmunoassay of human thyroglobulin: effect of antithyroglobulin autoantibodies

    Energy Technology Data Exchange (ETDEWEB)

    Schneider, A.B.; Pervos, R.

    1978-07-01

    This study was designed to investigate quantitatively the interference of thyroglobulin autoantibodies in the RIA of human thyroglobulin (hTG). Anti-hTG autoantibodies were combined with purified hTG to produce samples with known antibody titers and hTG concentrations. These samples were analyzed in the RIA. By using anti-human globulin serum it was first shown that immune complexes formed between labeled hTG and human anti-hTG. It was then shown that the most important factor in determining the direction of the interference was the specificity of the precipitating (second) antiserum with respect to these immune complexes. When the precipitating antiserum was specific, i.e. did not recognize human antibodies, the immune complexes remained in the supernatant and the measured hTG concentration was falsely elevated. When the precipitating antiserum cross-reacted with human antibodies, the direction of the interference depended on the sample volume. At small volumes there was false depression while at large volumes there was false elevation of apparent hTG levels, depending on the capacity of the precipitating antiserum to combine with human antibodies. Anti-hTG titers far below those detected by the tanned-red cell hemagglutination test had very large effects, to the point where measurements of hTG could not be made, when a cross-reactive precipitating antiserum was used. Therefore, the procedure which investigators have used until now, to exclude samples with anti-hTG hemagglutination titers above an arbitrary limit, is not adequate. It is necessary, until methods are developed which avoid the problem of autoantibody interference, to characterize each assay to determine the limits of anti-hTG that can be tolerated. The factors which influence anti-hTG interference in the hTG RIA are (1) the specificity of the precipitating antiserum, (2) the sample volume, (3) the maximum tracer binding, and (4) the anti-hTG titer.

  18. [Amino acid composition of rice grain proteins].

    Science.gov (United States)

    Peruanskiĭ, Iu V; Savich, I M

    1976-01-01

    The composition of the major reserve proteins of rice grain--globulins, prolamines and glutelins--was examined in four rice varieties (Dubovsky 129, Kuban 3, Alakul, Ushtobinsky). Globulins proved to be most heterogeneous whereas glutelins appeared to be least heterogeneous. In regards to the ratio of components globulins showed high variability and glutelins displayed high stability. PMID:1005365

  19. SwissProt search result: AK107271 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK107271 002-125-H12 (P14812) 12S seed storage globulin 2 precursor [Contains: 12S seed storage... globulin 2 acidic chain; 12S seed storage globulin 2 basic chain] SSG2_AVESA 1e-159 ...

  20. SwissProt search result: AK107343 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK107343 002-126-G05 (P14812) 12S seed storage globulin 2 precursor [Contains: 12S seed storage... globulin 2 acidic chain; 12S seed storage globulin 2 basic chain] SSG2_AVESA 1e-161 ...

  1. SwissProt search result: AK107343 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK107343 002-126-G05 (P12615) 12S seed storage globulin 1 precursor [Contains: 12S seed storage... globulin 1 acidic chain; 12S seed storage globulin 1 basic chain] SSG1_AVESA 1e-164 ...

  2. SwissProt search result: AK243018 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK243018 J090098O20 (P14812) 12S seed storage globulin 2 precursor [Contains: 12S seed storage... globulin 2 acidic chain; 12S seed storage globulin 2 basic chain] SSG2_AVESA 1e-160 ...

  3. SwissProt search result: AK107271 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK107271 002-125-H12 (P19084) 11S globulin seed storage protein G3 precursor (Helia...nthinin G3) [Contains: 11S globulin seed storage protein G3 acidic chain; 11S globulin seed storage protein G3 basic chain] 11S3_HELAN 6e-79 ...

  4. SwissProt search result: AK107314 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK107314 002-126-D11 (P19084) 11S globulin seed storage protein G3 precursor (Helia...nthinin G3) [Contains: 11S globulin seed storage protein G3 acidic chain; 11S globulin seed storage protein G3 basic chain] 11S3_HELAN 5e-79 ...

  5. SwissProt search result: AK121775 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK121775 J033092N18 (P12615) 12S seed storage globulin 1 precursor [Contains: 12S seed storage... globulin 1 acidic chain; 12S seed storage globulin 1 basic chain] SSG1_AVESA 2e-20 ...

  6. SwissProt search result: AK119657 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK119657 002-130-H05 (O23880) 13S globulin seed storage protein 2 precursor (Legumi...n-like protein 2) [Contains: 13S globulin seed storage protein 2 acidic chain; 13S globulin seed storage protein 2 basic chain] 13S2_FAGES 2e-69 ...

  7. SwissProt search result: AK064478 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK064478 002-111-A06 (O23880) 13S globulin seed storage protein 2 precursor (Legumi...n-like protein 2) [Contains: 13S globulin seed storage protein 2 acidic chain; 13S globulin seed storage protein 2 basic chain] 13S2_FAGES 1e-32 ...

  8. SwissProt search result: AK243018 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK243018 J090098O20 (O23878) 13S globulin seed storage protein 1 precursor (Legumin...-like protein 1) [Contains: 13S globulin seed storage protein 1 acidic chain; 13S globulin seed storage protein 1 basic chain] 13S1_FAGES 2e-66 ...

  9. SwissProt search result: AK107343 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK107343 002-126-G05 (O23878) 13S globulin seed storage protein 1 precursor (Legumi...n-like protein 1) [Contains: 13S globulin seed storage protein 1 acidic chain; 13S globulin seed storage protein 1 basic chain] 13S1_FAGES 4e-62 ...

  10. SwissProt search result: AK242284 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK242284 J075189I18 (P12615) 12S seed storage globulin 1 precursor [Contains: 12S seed storage... globulin 1 acidic chain; 12S seed storage globulin 1 basic chain] SSG1_AVESA 0.0 ...

  11. SwissProt search result: AK242009 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK242009 J075105H10 (P14812) 12S seed storage globulin 2 precursor [Contains: 12S seed storage... globulin 2 acidic chain; 12S seed storage globulin 2 basic chain] SSG2_AVESA 1e-159 ...

  12. SwissProt search result: AK243018 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK243018 J090098O20 (O23880) 13S globulin seed storage protein 2 precursor (Legumin...-like protein 2) [Contains: 13S globulin seed storage protein 2 acidic chain; 13S globulin seed storage protein 2 basic chain] 13S2_FAGES 2e-84 ...

  13. SwissProt search result: AK242284 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK242284 J075189I18 (O23880) 13S globulin seed storage protein 2 precursor (Legumin...-like protein 2) [Contains: 13S globulin seed storage protein 2 acidic chain; 13S globulin seed storage protein 2 basic chain] 13S2_FAGES 2e-92 ...

  14. SwissProt search result: AK061022 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK061022 006-204-D01 (O23878) 13S globulin seed storage protein 1 precursor (Legumi...n-like protein 1) [Contains: 13S globulin seed storage protein 1 acidic chain; 13S globulin seed storage protein 1 basic chain] 13S1_FAGES 2e-16 ...

  15. SwissProt search result: AK107314 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK107314 002-126-D11 (P14812) 12S seed storage globulin 2 precursor [Contains: 12S seed storage... globulin 2 acidic chain; 12S seed storage globulin 2 basic chain] SSG2_AVESA 1e-178 ...

  16. SwissProt search result: AK064478 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK064478 002-111-A06 (O23878) 13S globulin seed storage protein 1 precursor (Legumi...n-like protein 1) [Contains: 13S globulin seed storage protein 1 acidic chain; 13S globulin seed storage protein 1 basic chain] 13S1_FAGES 2e-35 ...

  17. SwissProt search result: AK121775 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK121775 J033092N18 (O23880) 13S globulin seed storage protein 2 precursor (Legumin...-like protein 2) [Contains: 13S globulin seed storage protein 2 acidic chain; 13S globulin seed storage protein 2 basic chain] 13S2_FAGES 3e-23 ...

  18. SwissProt search result: AK243000 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK243000 J090095N02 (P12615) 12S seed storage globulin 1 precursor [Contains: 12S seed storage... globulin 1 acidic chain; 12S seed storage globulin 1 basic chain] SSG1_AVESA 0.0 ...

  19. SwissProt search result: AK242312 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK242312 J075195B06 (P19084) 11S globulin seed storage protein G3 precursor (Helian...thinin G3) [Contains: 11S globulin seed storage protein G3 acidic chain; 11S globulin seed storage protein G3 basic chain] 11S3_HELAN 1e-68 ...

  20. SwissProt search result: AK242245 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK242245 J075180O04 (P12615) 12S seed storage globulin 1 precursor [Contains: 12S seed storage... globulin 1 acidic chain; 12S seed storage globulin 1 basic chain] SSG1_AVESA 1e-178 ...

  1. SwissProt search result: AK242312 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK242312 J075195B06 (O23880) 13S globulin seed storage protein 2 precursor (Legumin...-like protein 2) [Contains: 13S globulin seed storage protein 2 acidic chain; 13S globulin seed storage protein 2 basic chain] 13S2_FAGES 2e-81 ...

  2. SwissProt search result: AK064478 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK064478 002-111-A06 (P12615) 12S seed storage globulin 1 precursor [Contains: 12S seed storage... globulin 1 acidic chain; 12S seed storage globulin 1 basic chain] SSG1_AVESA 5e-47 ...

  3. SwissProt search result: AK242872 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK242872 J090076M21 (O23878) 13S globulin seed storage protein 1 precursor (Legumin...-like protein 1) [Contains: 13S globulin seed storage protein 1 acidic chain; 13S globulin seed storage protein 1 basic chain] 13S1_FAGES 5e-62 ...

  4. SwissProt search result: AK105316 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK105316 001-117-E07 (P12615) 12S seed storage globulin 1 precursor [Contains: 12S seed storage... globulin 1 acidic chain; 12S seed storage globulin 1 basic chain] SSG1_AVESA 6e-75 ...

  5. SwissProt search result: AK119657 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK119657 002-130-H05 (P14812) 12S seed storage globulin 2 precursor [Contains: 12S seed storage... globulin 2 acidic chain; 12S seed storage globulin 2 basic chain] SSG2_AVESA 6e-75 ...

  6. SwissProt search result: AK243000 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK243000 J090095N02 (P19084) 11S globulin seed storage protein G3 precursor (Helian...thinin G3) [Contains: 11S globulin seed storage protein G3 acidic chain; 11S globulin seed storage protein G3 basic chain] 11S3_HELAN 3e-80 ...

  7. SwissProt search result: AK242872 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK242872 J090076M21 (O23880) 13S globulin seed storage protein 2 precursor (Legumin...-like protein 2) [Contains: 13S globulin seed storage protein 2 acidic chain; 13S globulin seed storage protein 2 basic chain] 13S2_FAGES 2e-86 ...

  8. SwissProt search result: AK107314 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK107314 002-126-D11 (O23878) 13S globulin seed storage protein 1 precursor (Legumi...n-like protein 1) [Contains: 13S globulin seed storage protein 1 acidic chain; 13S globulin seed storage protein 1 basic chain] 13S1_FAGES 3e-72 ...

  9. SwissProt search result: AK107285 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK107285 002-126-B05 (P12615) 12S seed storage globulin 1 precursor [Contains: 12S seed storage... globulin 1 acidic chain; 12S seed storage globulin 1 basic chain] SSG1_AVESA 2e-81 ...

  10. SwissProt search result: AK107314 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK107314 002-126-D11 (O23880) 13S globulin seed storage protein 2 precursor (Legumi...n-like protein 2) [Contains: 13S globulin seed storage protein 2 acidic chain; 13S globulin seed storage protein 2 basic chain] 13S2_FAGES 4e-90 ...

  11. SwissProt search result: AK242872 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK242872 J090076M21 (P12615) 12S seed storage globulin 1 precursor [Contains: 12S seed storage... globulin 1 acidic chain; 12S seed storage globulin 1 basic chain] SSG1_AVESA 1e-178 ...

  12. SwissProt search result: AK243000 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK243000 J090095N02 (O23880) 13S globulin seed storage protein 2 precursor (Legumin...-like protein 2) [Contains: 13S globulin seed storage protein 2 acidic chain; 13S globulin seed storage protein 2 basic chain] 13S2_FAGES 7e-93 ...

  13. SwissProt search result: AK107285 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK107285 002-126-B05 (O23880) 13S globulin seed storage protein 2 precursor (Legumi...n-like protein 2) [Contains: 13S globulin seed storage protein 2 acidic chain; 13S globulin seed storage protein 2 basic chain] 13S2_FAGES 6e-46 ...

  14. SwissProt search result: AK107343 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK107343 002-126-G05 (O23880) 13S globulin seed storage protein 2 precursor (Legumi...n-like protein 2) [Contains: 13S globulin seed storage protein 2 acidic chain; 13S globulin seed storage protein 2 basic chain] 13S2_FAGES 1e-84 ...

  15. SwissProt search result: AK242284 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK242284 J075189I18 (O23878) 13S globulin seed storage protein 1 precursor (Legumin...-like protein 1) [Contains: 13S globulin seed storage protein 1 acidic chain; 13S globulin seed storage protein 1 basic chain] 13S1_FAGES 8e-66 ...

  16. SwissProt search result: AK058320 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK058320 001-014-A11 (P14812) 12S seed storage globulin 2 precursor [Contains: 12S seed storage... globulin 2 acidic chain; 12S seed storage globulin 2 basic chain] SSG2_AVESA 1e-15 ...

  17. SwissProt search result: AK121775 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK121775 J033092N18 (O23878) 13S globulin seed storage protein 1 precursor (Legumin...-like protein 1) [Contains: 13S globulin seed storage protein 1 acidic chain; 13S globulin seed storage protein 1 basic chain] 13S1_FAGES 7e-20 ...

  18. The struggle against imperial punditry and reactionary anti-humanism: critical pedagogy, latina/o education, and class struggle in the age of empire = A luta contra o império da erudição e o anti-humanismo reacionário: pedagogia crítica, educação latina e luta de classe na idade do império

    Directory of Open Access Journals (Sweden)

    McLaren, Peter

    2005-01-01

    Full Text Available Muitas das mais usuais idéias do trabalho da educação crítica nos Estados Unidos, juntamente com o trabalho em campos afins, agora aparecem lamentavelmente separadas das especificidades históricas e determinações básicas da sociedade capitalista. Neste artigo, McLaren e Jaramillo presumem como um fato uma visão social alternativa do que o mundo poderia e deveria parecer sem o conceito e valor do capital. Usando as políticas da educação latina como um exemplo ilustrativo das formas como o império impacta uma das mais amplas populações de estudantes das minorias nos Estados Unidos, McLaren e Jaramillo saem em defesa de uma pedagogia crítica revelocionária contra um anti-humanismo reacionário. Até certo ponto, este artigo inclui considerações prévias da pedagogia crítico revolucionária como uma dialética da praxis fundada nos princípios do marxismo-humanismo. A pedagogia crítica revolucionária é, finalmente, filosoficamente dirigida, formulada para ajudar professores e estudantes a compreender a especificidade do concreto dentro da totalidade do universal

  19. A comparison study on the detection of anti-human immunodeficiency virus type 1 (HTV-1) antibodies in different populations with a new rapid test using oral mucosal transudate samples versus enzyme linked immunosorbent assay (ELISA) using serum samples%口腔黏膜渗出液快速诊断试剂与血清ELISA试剂检测不同人群HIV-1抗体的评价

    Institute of Scientific and Technical Information of China (English)

    吴焱; 伦文辉; 王克荣; 韩晶; 赵红心; 曾辉; 徐克沂; 刘彦春; 闫会文; 李兴旺

    2011-01-01

    目的探讨口腔黏膜渗出液(OMT)快速诊断试剂与酶联免疫吸附试验(ELISA)试剂检测不同人群HIV-1抗体的一致性.方法对已经过生物梅里埃ELISA试剂检测血清HIV-1抗体阳性并经蛋白印迹(WB)试验确诊的HIV感染者200例(HIV阳性组),和经过生物梅里埃ELISA试剂检测血清HIV-1抗体阴性的健康人群600例(HIV阴性组)采集OMT标本,使用OMT快速诊断试剂进行HIV-1抗体检测.同时评价口腔黏膜渗出液快速诊断试剂在实际应用中的影响因素.结果 HIV阳性组200例中198例OMT标本检测为阳性,其中192例(96%)检测线清楚,易做阳性结果判断.4例(2%)"模糊",2例(1%)"很模糊",需经专业人员判断;2例(1%)检测线"看不见",为阴性.HIV阴性组600例OMT标本检测HIV抗体全为阴性.结论口腔黏膜渗出液快速诊断试剂与生物梅里埃血清ELISA诊断试剂检测HIV-1抗体相比,在HIV阳性标本中检测一致性为99.0%,在HIV阴性标本中检测一致性为100%,总体一致性为99.75%.%Objective To evaluate the consistence in the detection of antibodies against HIV-1 between a new rapid test using oral mucosal transudate (OMT) samples and ELISA using serum samples. Methods Two-hundred patients who were positive for anti-HIV-1 antibodies by serum ELISA and confirmed by Western blot to be infected with HIV, and 600 healthy human controls negative for anti-HIV-1 antibodies by serum ELISA, were eligible for this study. OMT samples were collected from these subjects and subjected to a rapid test for anti-HIV-1 antibodies. The factors influencing the performance of the rapid test were analyzed. Results Of the 200 OMT specimens from HIV-infected patients, 198 showed positive reaction, 2 showed negative reaction. Among the 198 positive reactions, 192 (96%) were "clear" and easy to make decisions, 4 (2%) were "faint", 2(1%) were "very faint" and required professionals to make decisions. The rapid test was negative in all the 600 OMT specimens from the control group. Conclusions The consistence in the detection of anti-HIV-1 antibodies between the OMT rapid test and serum ELISA was 99% in HIV-positive specimens, 100% in HIV-negative specimens, and 99.75% in all the specimens.

  20. Immunoelectrophoresis - blood

    Science.gov (United States)

    IEP - serum; Immunoglobulin electrophoresis - blood; Gamma globulin electrophoresis; Serum immunoglobulin electrophoresis ... A blood sample is needed. For information on how this is done, see: Venipuncture

  1. Screening Analysis of Irregular Antibodies of 5014 Blood Donors in Hohhot Area%呼和浩特地区5014例献血人群不规则抗体筛查分析

    Institute of Scientific and Technical Information of China (English)

    张伟; 张俊玲; 尚锦清; 白俊凤

    2009-01-01

    [Objective]To analyze the frequency and distribution of irregular antibodies in Hohhot area so as to ensure the safety of blood transfusion. [Methods]Blood donors of Hohhot area were randomly selected in 2008; antibody screening cells, polybrene test and anti-human globulin test were adopted for antibody screening.[Results]The irregular antibodies were found in 9 samples(0.18%). The frequency of irregular antibodies in female was higher than that in male(P<0.01)and Rh antibodies such as anti-D, anti-E, and anti-Ec C (55.6%)were common. One sample of Le antibodies was failed to be found by polybrene test.[Conclusion]Because of irregular antibodies resulting in hemolytic transfusion reaction, the investigation of irregular antibodies is very important for ensuring blood transfusion. Antibody screening must be done for massive plasma transfusion of female donors, severe patients and infants to ensure safety.%目的 分析呼和浩特地区不规则抗体的频率与分布特征,以确保输血安全.方法 于2008年对呼和浩特地区献血者中随机抽取5014例,采用抗体筛选细胞、聚凝胺试验和抗人球蛋白试验作抗体筛查.结果 筛出不规则抗体9例,占0.18%.女性不规则抗体者多于男性(P<0.01),多为Rh抗体(抗D、抗E和抗C占55.6%).聚凝胺法漏检1例Le抗体.结论 由于不规则抗体可以导致溶血性输血反应和新生儿溶血病,因此调查该地区不规则抗体对安全输血十分重要.对女性献血者或给重危病人、小儿大量输注的血浆尤应做抗体筛选以确保安全输血.

  2. Efficacy of total lymphoid irradiation for chronic allograft rejection following bilateral lung transplantation

    International Nuclear Information System (INIS)

    Purpose: To assess the safety and efficacy of total lymphoid irradiation (TLI) in patients experiencing chronic rejection following bilateral lung transplantation (BLT). Patients and Materials: Eleven patients received TLI for chronic allograft rejection (bronchiolitis obliterans syndrome) refractory to conventional treatment modalities. Radiation therapy (RT) was prescribed as 8 Gy delivered in 10 0.8-Gy fractions, 2 fractions/week, via mantle, paraaortic, and inverted-Y fields. Serial pre- and post-RT pulmonary function values, complete blood counts, and immunosuppressive augmentation requirements [use of methylprednisolone, murine anti-human mature T-cell monoclonal antibody (OKT3), polyclonal antithymocyte globulin (ATG), and tacrolimus] were monitored. Results: In the 3 months preceding TLI, the average decrease in forced expiratory volume in 1 s (FEV1) was 34% (range 0-75%) and the median number of immunosuppression augmentations was 3 (range 0-5). Only 4 of 11 patients completed all 10 TLI treatment fractions. Reasons for discontinuation included progressive pulmonary decline (four patients), worsening pulmonary infection (two patients), and persistent thrombocytopenia (one patient). Seven of the 11 patients failed within 8 weeks of treatment cessation. One patient had unabated rejection and received bilateral living related-donor transplants; he is alive and well. Six patients died. Two of these deaths were due to pulmonary infection from organisms isolated prior to the start of RT; the other four deaths were from progressive pulmonary decline. The four remaining patients had durable positive responses to TLI (mean follow-up of 47 weeks; range 24-72). Comparing the 3 months preceding RT to the 3 months following treatment, these four patients had improvements in average FEV1 (40% decline vs. 1% improvement) and fewer median number of immunosuppressive augmentations (3.5 vs. 0). None of these patients has developed lymphoproliferative disease or has died

  3. Determinação da atividade in vitro de inibidores de tripsina em feijão (Phaseolus vulgaris L. preto, albumina e globulina
    Determination of the trypsin inhibitor activity in vitro of the black bean (Phaseolus vulgaris L. albumin and globulin

    Directory of Open Access Journals (Sweden)

    M. S. L. DUARTE

    2010-12-01

    Full Text Available

    Evidências científicas vêm demonstrando, nas últimas décadas, que o feijão possui uma baixa digestibilidade devido a diversos fatores, tais como estrutura compacta de suas proteínas e fatores antinutricionais. Este trabalho teve como objetivo determinar a atividade de inibidor de tripsina em feijão preto e nas suas frações proteicas, albumina e globulina. O resultado mostrou que a albumina apresentou alta taxa de inibição de atividade enzimática (99,81% em comparação, a globulina (31,98% e que o grão integral apresentou a menor taxa de inibição enzimática (14,33.

  4. The effect of intravenous immune globulin on Toll-like receptor 4 expression of brain tissues in fetal rats with intrau-terine infection%静脉注射免疫球蛋白对宫内感染胎鼠脑组织Toll样受体4表达影响的实验研究

    Institute of Scientific and Technical Information of China (English)

    戴梦颖; 李晓捷; 孙奇峰

    2013-01-01

    目的 观察静脉注射免疫球蛋白(IVIG)对围产期炎症暴露的胎鼠及子鼠脑组织Toll样受体4(TLR4)的影响.方法 将40只SD大鼠孕鼠随机分成干预组、实验组、对照组3组.干预组16只于受孕第17天给予脂多糖(LPS)腹腔注射,剂量为350 μg/kg,制备宫内感染模型,取雌鼠后尾静脉,于造模成功后3 h按2 g/kg注射IVIG 1次.实验组16只以相同的方法制备孕鼠宫内感染模型.对照组8只,在大鼠孕第17天给予等量生理盐水腹腔注射.取腹腔注射后的第24小时(孕第18天)、孕第19天、孕第21天以及生后1 d.用苏木精-伊红染色法观察各组胎盘及各时间点脑组织的病理改变,比较各组胎鼠的体质量,用PCR法测得各组子鼠脑中TLR4-mRNA的含量,用免疫组织化学法观察各组各时间点脑组织TLR4的表达情况.结果 实验组孕鼠胎盘及子宫壁充血水肿,白细胞浸润,子鼠脑白质水肿,并出现形态幼稚的细胞,甚至可见局灶性出血.实验组及干预组子鼠体质量出现不同程度减轻,与对照组比较,差异有统计学意义(P<0.01).实验组与干预组子鼠脑内TLR4-mRNA及TLR4的表达量在不同时间点出现不同程度的增高.结论 宫内LPS感染可导致胎儿脑组织炎性反应,及时使用IVIG可以减少小胶质细胞的持续活化从而抑制胎儿脑组织的炎症反应,降低脑损伤的程度,达到脑保护的作用.

  5. 健康人与慢性牙周炎患者唾液蛋白质量浓度及白蛋白与球蛋白比值的变化%Variations in protein concentration and albumin/globulin ratio of whole unstimulated saliva obtained from healthy ;people and patients with chronic periodontitis

    Institute of Scientific and Technical Information of China (English)

    崔博淼; 余宇; 袁雯雯; 周闻捷; 周学东; 张平

    2015-01-01

    目的:对健康人1 d内唾液总蛋白、白蛋白、球蛋白质量浓度的变化及白蛋白与球蛋白比值(A/G)进行检测、分析,确定唾液蛋白成分较稳定的时间段,并比较此时间段健康人与慢性牙周炎患者非刺激性全唾液中的蛋白质量浓度及A/G的变化,为以唾液为基础的疾病诊断学方法建立和临床应用提供参照。方法分别采集37名健康受试者(健康组)8:00、9:30、11:30、13:00、16:30、21:00的全唾液,并取24名慢性牙周炎患者(牙周炎组)早晨的全唾液。使用BCA法在酶标仪上检测总蛋白质量浓度,用GF-D800型半自动生化分析仪检测唾液白蛋白的质量浓度,然后计算球蛋白质量浓度及A/G。使用SPSS 19.0统计软件对所得数据进行统计学分析。结果唾液蛋白成分在早晨(8:00)空腹时较为稳定。在这段时间,健康组总蛋白(1 354.35±389.52)μg·mL-1、白蛋白(139.55±27.19)μg·mL-1、球蛋白(1 211.80±360.73)μg·mL-1、A/G 0.126 3±0.041 7;牙周炎组总蛋白(2 611.56±231.62)μg·mL-1、白蛋白(296.27±17.34)μg·mL-1、球蛋白(2 315.69±221.67)μg·mL-1、A/G 0.156 2±0.017 3。健康组总蛋白、白蛋白、球蛋白的质量浓度在1 d内不同时间存在明显差异(P<0.05),主要体现在饭前与饭后的差异,但A/G在1 d内基本稳定。对健康组与牙周炎组的唾液蛋白及A/G进行比较,牙周炎组的唾液总蛋白、白蛋白、球蛋白质量浓度较健康组明显升高,但A/G无明显差异。结论唾液蛋白成分在早晨空腹时较其他时段稳定,可为样本采集时间提供参考。慢性牙周炎患者的唾液总蛋白、白蛋白、球蛋白质量浓度较健康人明显升高。%Objective We detected and analyzed the clinical values of total protein (TP), albumin (ALB), globulin (GLB), and ALB/GLB ratio (A/G) of whole unstimulated saliva of healthy people to determine the time of day when saliva composi-tion is relatively stable. We compared the protein concentration and A/G of whole unstimulated saliva of patients with chronic periodontitis with those of healthy volunteers to provide references for diagnostic methods and clinical applications of saliva. Methods   The whole saliva of 37 healthy subjects were collected at 8:00, 9:30, 11:30, 13:00, 16:30, and 21:00. Meanwhile, the whole saliva of 24 patients with periodontitis was collected in the morning. Bicinchoninic acid method was used to detect the TP content. Saliva ALB was detected by GF-D800 semi-automatic biochemical analyzer, and the GLB and A/G were cal-culated. Finally, the results were statistically analyzed using SPSS 19.0. Results   Salivary protein compositions were stable in the morning on an empty stomach. Healthy people:TP, (1 354.35±389.52)μg·mL-1;ALB, (139.55±27.19)μg·mL-1; GLB, (1 211.80±360.73)μg·mL-1;A/G, 0.126 3±0.041 7. Subjects with chronic periodontitis:TP, (2 611.56±231.62)μg·mL-1; ALB, (296.27±17.34)μg·mL-1;GLB, (2 315.69±221.67)μg·mL-1; A/G, 0.156 2±0.017 3. The contents of TP, ALB, and GLB in healthy individuals at different periods within a day showed significant differences (P<0.05), which were mainly reflected in the levels before and after meals. No significant difference was detected in A/G. The concentrations of TP, ALB, and GLB were significantly increased in patients with chronic periodontitis compared with those in healthy volunteers. However, no significant difference existed in A/G. Conclusion   Salivary protein compositions are more stable in the morning than in other periods. Thus, mornings can be set as the time of specimen collec-tion in future research. The concentrations of TP, ALB, and GLB in patients with chronic periodontitis are higher than those in healthy people.

  6. Relationship Between Analysis of Serum Immune Globulin and Complement C3 of Esophageal Carcinoma Patients with Radiotherapy Prognosis%食管癌患者血清免疫球蛋白与补体C3测定对放射治疗预后分析

    Institute of Scientific and Technical Information of China (English)

    成国建

    2006-01-01

    目的探讨食管癌患者放疗前血清免疫球蛋白IgG、IgA、IgM与补体C3的含量与放射治疗预后的关系.方法用速率散射比浊法对血清免疫球蛋白与补体C3正常组与异常组进行对比.结果正常组放疗后局控率及1年生存率均高于异常组.结论血清中免疫球蛋白和补体C3含量的异常可能影响食管癌患者放射治疗预后.

  7. The efficacy and safety of a new reduced-toxicity conditioning with 4 days of once-daily 100 mg/m(2) intravenous busulfan associated with fludarabine and antithymocyte globulins prior to allogeneic stem cell transplantation in patients with high-risk myelodysplastic syndrome or acute leukemia.

    Science.gov (United States)

    Wanquet, Anne; Crocchiolo, Roberto; Furst, Sabine; Granata, Angela; Faucher, Catherine; Devillier, Raynier; Harbi, Samia; Lemarie, Claude; Calmels, Boris; Vey, Norbert; Weiller, Pierre Jean; Chabannon, Christian; Castagna, Luca; Blaise, Didier; El-Cheikh, Jean

    2016-10-01

    The optimal intensity of myeloablation associated with a reduced-toxicity conditioning (RTC) regimen in order to decrease the relapse rate without increasing non-relapse mortality (NRM), is not well established yet. This retrospective analysis was done on 30 patients with hematological malignancies. The aim was to assess the safety of a RTC regimen based on the busulfan at a dose of 100 mg/m(2)/d intravenously for 4 d, fludarabine at a dose of 30 mg/m(2)/d for 5 d, and anti-thymoglobulins at a dose of 2.5 mg/kg/d for 2 d. The cumulative incidences of grade 2-4 acute graft-versus-host disease (GVHD) and all grades chronic GVHD were 37% and 42%, respectively. Median 1-year overall survival and disease-free survival were 66% and 50%, respectively. At 1 year, the cumulative incidence of relapse/disease progression was 33%. NRM was 3% and 17% at day 100 and 1 year, respectively. This RTC conditioning regimen can lead to a long-term disease control. Moreover, it appears to be safe with a low NRM rate among high-risk patients. PMID:26885686

  8. Caracterização e hidrólise in vitro da globulina principal de grão-de-bico (Cicer arietinum L.), var. IAC-Marrocos Characterization and in vitro tryptic hydrolysis of the major globulin from chickpea (Cicer arietinum L.)

    OpenAIRE

    NEVES Valdir A.; Maraiza A. da Silva; LOURENÇO Euclides J.

    2004-01-01

    No presente estudo procedeu-se ao isolamento e caracterização da fração globulina majoritária (11 S) de grão-de-bico, var. IAC-Marrocos. A globulina majoritária extraída foi isolada por cromatografia de filtração em gel e de troca-iônica mostrando apenas uma banda de proteína na eletroforese em gel de poliacrilamida. A globulina majoritária, após passagem em coluna de Sephadex, revelou duas bandas protéicas de 55 e 52,5kDa e três bandas menores em gel de poliacrilamida dodecilsulfato de sódio...

  9. The clinic curative effect observation of rifampicin infection united with human gramma globulin to cure refractory hypostatic pneumonia%利福平注射液联合丙种球蛋白治疗难治性坠积性肺炎临床疗效观察

    Institute of Scientific and Technical Information of China (English)

    逯振想; 何志勇; 魏红; 张熙曼

    2011-01-01

    目的 探讨利福平注射液联合丙种球蛋白治疗难治性坠积性肺炎的临床疗效.方法 将90例患者随机分为观察组和对照组,每组45例,观察组每日以利福平注射液450mg加入5%葡萄糖500ml中静脉滴注,丙种球蛋白0.4g/(kg·d)静脉滴注5天,对照组联合应用头孢哌酮舒巴坦钠3.0及左氧氟沙星0.4各加入0.9%氯化钠250ml静脉滴注,疗程均为10天.结果 观察组显效15例,有效23例,无效7例,总有效率84.4%.对照组显效8例,有效16例,无效21天,总有效率53.3%.两组对比差异有统计学意义(P<0.01).结论 利福平注射液联合丙种球蛋白治疗难治性坠积性肺炎疗效肯定,值得临床应用.

  10. 78 FR 65904 - Permanent Discontinuance or Interruption in Manufacturing of Certain Drug or Biological Products

    Science.gov (United States)

    2013-11-04

    ...(D) Immune Globulin and Hepatitis B Immune Globulin; Coagulation Factor VIIa (Recombinant); and..., or mitigate shortages of these products. b. Vaccines. We are proposing to apply section 506C of the FD&C Act to all biological products, including vaccines. Under section 506C(i)(3)(B) of the FD&C...

  11. Resistant Kawasaki disease, case report and literatures review

    Directory of Open Access Journals (Sweden)

    M.J. Saffar

    2006-01-01

    Full Text Available A child with aggressive and resistant Kawasaki disease with coronary aneurysm is described. Despite two doses of immune globulin and high dose of aspirin the patient was treated with the third dose of immune globulin. The patient apparently responded to normalization of symptoms and signs, with no more progression of coronary artery abnormalities.

  12. Main: SEF1MOTIF [PLACE

    Lifescience Database Archive (English)

    Full Text Available inding motif; sequence found in 5'-upstream region (-640; -765) of soybean beta-conglicinin (7S globulin) ge...ne; W=A/T; SOYBEAN; STORAGE PROTEIN; 7S; GLOBULIN; BETA-CONGLICININ; seed; soybean (Glycine max) ATATTTAWW ...

  13. Inter-laboratory optimization of protein extraction, separation, and fluorescent detection of endogenous rice allergens

    DEFF Research Database (Denmark)

    Satoh, Rie; Teshima, Reiko; Kitta, Kazumi;

    2016-01-01

    In rice, several allergens have been identified such as the non-specific lipid transfer protein-1, the α-amylase/trypsin-inhibitors, the α-globulin, the 33 kDa glyoxalase I (Gly I), the 52-63 kDa globulin, and the granule-bound starch synthetase. The goal of the present study was to define optima...

  14. Isolation and characterization of proteins from chia seeds (Salvia hispanica L.).

    Science.gov (United States)

    Sandoval-Oliveros, María R; Paredes-López, Octavio

    2013-01-01

    Chia ( Salvia hispanica L.) is a plant that produces seeds rich in some nutraceutical compounds with high protein content, but little is known about them; for this reason the aim of this study was to characterize the seed storage proteins. Protein fractions were extracted from chia seed flour. The main protein fraction corresponded to globulins (52%). Sedimentation coefficient studies showed that the globulin fraction contains mostly 11S and 7S proteins. The molecular sizes of all the reduced fractions were about 15-50 kDa. Electrophoretic experiments under native conditions exhibited four bands of globulins in the range of 104-628 kDa. The denaturation temperatures of crude albumins, globulins, prolamins, and glutelins were 103, 105, 85.6, and 91 °C, respectively; albumins and globulins had relatively good thermal stability. Selected globulin peptides by mass spectrometry showed homology to sesame proteins. A good balance of essential amino acids was found in the seed flour and globulins, especially of methionine+cysteine.

  15. Physiological responses of the adult male collared peccary, Tayassu tajacu (Tayassuidae), to severe dietary restriction.

    Science.gov (United States)

    Lochmiller, R L; Hellgren, E C; Varner, L W; Greene, L W; Amoss, M S; Seager, S W; Grant, W E

    1985-01-01

    Metabolic and hormonal responses of eight adult male collared peccaries (Tayassu tajacu) to an ad libitum diet intake, or 25% of an ad libitum intake, were examined. Blood samples for hematological, serum-biochemical and hormonal profiles were collected at three week intervals during the nine week experiment starting 4 August 1983. Males fed on the restricted diet lost an average of 26% of their body weight during the trial, compared to a slight weight gain for those fed ad libitum. Characteristics of the red and white blood cell populations were not influenced by diet intake, with the exception of mean corpuscular volume, which was consistently lower amongst males fed on the restricted diet. Restricted food intake resulted in significantly elevated serum values for urea nitrogen, urea nitrogen:creatinine, urea index, alpha globulin:beta globulin, gamma globulin:albumin, nonesterified fatty acids, alkaline phosphatase and lactate dehydrogenase isozymes (LD1 and LD2). Restricted food intake resulted in significantly lowered serum values for total alpha globulin, alpha-1 globulin, total beta globulin, beta-1 globulin, beta-2 globulin, glucose, triglycerides, calcium, magnesium, sodium, chloride, copper and triiodothyronine. Serum levels of creatinine, total protein, albumin, alpha-2 globulin, uric acid, total bilirubin, cholesterol, aspartate aminotransferase, alanine aminotransferase, gamma glutamyltransferase, lactate dehydrogenase, phosphorus, calcium:phosphorus, potassium, iron, zinc and thyroxine were unaffected by diet intake level. Semen evaluation indicated spermatogenesis was not affected by dietary restriction despite reductions in scrotal circumference and ejaculate gel volume. Serum testosterone levels were significantly lower among males fed on the restricted diet after nine weeks. These data suggest male libido might be depressed during poor range conditions, while maintenance of spermatogenesis might permit them to take immediate advantage of improved

  16. Cotyledon storage proteins as markers of the genetic diversity in Castanea sativa Miller.

    Science.gov (United States)

    Alvarez, J B; Muñoz-Diez, C; Martín-Cuevas, A; Lopez, S; Martín, L M

    2003-08-01

    This study has been to analyse the useful nut globulin proteins as a marker of the genetic diversity in Castanea sativa. The evaluated populations were highly polymorphic for the globulins, being detected up to 35 polymorphic bands with a wide distribution among all the evaluated populations. Taken together for populations from all the chestnut regions, about 39.3% of total allelic variation was distributed among the populations. The estimates of genetic similarity between populations were clearly associated with the collecting site. This method of analysis of the nut storage proteins (globulins) could be a useful tool for the evaluation of genetic diversity in this and other species of the Fagaceae.

  17. Serum proteins, trace metals and phosphatases in psoriasis

    Directory of Open Access Journals (Sweden)

    Bhatnagar M

    1994-01-01

    Full Text Available Serum proteins, zinc, copper, acid phosphatase (AcPase and alkaline phosphatase (AlPase were studied in both active and remission phases of psoriasis. Data were compared with healthy controls, ?1, ? and ? globulins were high in active phase while ?1 and ? globulins were at par in remission phase. Serum copper was low but zinc and alkaline phosphatase were significantly high in both active and remission phases of the disease. Acid phosphatase level was at par in all the experimental groups. Study suggests a positive correlation of globulin, zinc and Alpase in active and remission phase of psoriasis.

  18. A Model System for Concurrent Detection of Antigen and Antibody Based on Immunological Fluorescent Method

    OpenAIRE

    2015-01-01

    This paper describes a combined antigen/antibody immunoassay implemented in a 96-well plate using fluorescent spectroscopic method. First, goat anti-human IgG was used to capture human IgG (model antigen); goat anti-human IgG (Cy3 or FITC) was used to detect the model antigen; a saturating level of model antigen was then added followed by unlabelled goat anti-human IgG (model antibody); finally, Cy3 labelled rabbit anti-goat IgG was used to detect the model antibody. Two approaches were appli...

  19. Study of plasma amino acid levels in children with autism: An Egyptian sample

    Directory of Open Access Journals (Sweden)

    Farida M. ElBaz

    2014-04-01

    Conclusions: Autistic children had lower levels of some plasma amino acids except for glycine and glutamic acids and phosphoserine were increased with normal serum levels of urea, ammonia, total proteins, albumin and globulins (alpha 1, alpha 2, beta and gamma.

  20. Alpha fetoprotein

    Science.gov (United States)

    Fetal alpha globulin; AFP ... Greater than normal levels of AFP may be due to: Cancer in testes , ovaries, biliary (liver secretion) tract, stomach, or pancreas Cirrhosis of the liver Liver cancer ...

  1. Kawasaki Disease (For Parents)

    Science.gov (United States)

    ... dry, cracked lips swollen tongue with a white coating and big red bumps (called "strawberry tongue") sore, ... child is treated with intravenous (IV) doses of gamma globulin (purified antibodies), an ingredient of blood that ...

  2. Kawasaki disease

    Science.gov (United States)

    ... mucous membranes in the mouth Strawberry tongue, white coating on the tongue, or visible red bumps on ... damage to the coronary arteries and heart. Intravenous gamma globulin is the standard treatment. It is given ...

  3. Hepatitis A, B, and C: Learn the Differences

    Science.gov (United States)

    ... woman is pregnant, she should be tested for hepatitis B (HBsAg blood test); infants born to HBV-infected mothers should be given HBIG (hepatitis B immune globulin) and vaccine within 12 hours of ...

  4. Diphtheria, Tetanus, Pertussis: Ask the Experts

    Science.gov (United States)

    ... pdf . I have an adult patient with controlled epilepsy who wishes to receive the Tdap vaccine. May ... a tetanus-prone wound) until the development of human tetanus immune globulin in the late 1950s. Equine ...

  5. 21 CFR 610.21 - Limits of potency.

    Science.gov (United States)

    2010-04-01

    ... Globulin (Human), 250 units of tetanus antitoxin per container. Antigens Cholera Vaccine, 8 units each of Inaba and Ogawa serotype antigens per milliliter. Pertussis Vaccine, 12 units per total human immunizing dose. Typhoid Vaccine, 8 units per milliliter....

  6. Drug: D08787 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available D08787 Drug Freeze-dried pepsin treated human normal immunoglobulin; Globulin (TN) ...43 Plasma preparations D08787 Freeze-dried pepsin treated human normal immunoglobulin PubChem: 96025470 ...

  7. Decrease of free thyroxine levels after controlled ovarian hyperstimulation

    NARCIS (Netherlands)

    A.F. Muller (Alex); A. Verhoeff; M.J. Mantel; F.H. de Jong (Frank); A. Berghout (Arie)

    2000-01-01

    textabstractControlled ovarian hyperstimulation could lead to opposing effects on thyroid function. Therefore, in a prospective study of 65 women undergoing controlled ovarian hyperstimulation, thyroid hormones, T4-binding globulin, TPO antibodies, gonadotropins, estrad

  8. Drug: D06458 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available s against pathologic organisms and parasites 63 Biological preparations 634 Human blood preparation...s 6343 Plasma preparations D06458 Human normal immunoglobulin (JP16); Globulin, immune (US

  9. 南阳市Rh D阴性稀有血型库的建立及临床意义

    Institute of Scientific and Technical Information of China (English)

    凌励

    2015-01-01

    Objective The establishment of a rare blood bank in Nanyang, and to explore its clinical signifi cance.Methods Establish Nanyang city rare blood type library retrieval, and explore the clinical signifi cance of the micro plate method of Nanyang City 81462 blood donation, Rh blood group antigen D for screening and Rh D negative samples and then at the beginning of the blood group screen with three different batches of IgG anti-D antibodies of anti human globulin (IAT) experiment validation. Initial screening and confi rmation tests were negative for D Rh negative blood group. D ABO negative Rh blood group and Rh blood group were also examined.Results All blood donors were screened and confi rmed that a total of 300 D Rh negative blood group, accounting for 0.37%, of which the type B blood is the most, the performance of ccdee based. The data of blood donors and the results of the examination were entered into the computer, the establishment of the Nanyang area D Rh negative rare blood group information database.Conclusion Rare blood group RH D negative information database for emergency treatment of RH D negative patients with blood provides a green life path, to prevent transfusion of Rh blood group incompatibility caused by acute hemolytic reaction and Rh blood group incompatibility of pregnancy induced hemolytic disease of the newborn is of far-reaching signifi cance.%目的:建立南阳市稀有血型检索库,并探讨其临床意义。方法采用微板法对南阳市81462名无偿献血者Rh D血型抗原进行初筛检查,对血型初筛Rh D阴性标本再用3个不同批号的IgG抗-D抗体试剂进行抗人球蛋白(IAT)实验确认。初筛和确认实验均为阴性者为Rh D阴性血型。同时检查Rh D阴性者的ABO血型以及Rh血型的表现型。结果所有献血者经筛选和确认共有300名Rh D阴性血型,占0.37%,其中B型血最多,表现型以ccdee为主。将献血者的资料和检查的结果输入计算

  10. Irregular Antibody Screening Preventive Effect in Hemolytic Transfusion Reaction%不规则抗体筛查在溶血性输血反应中的预防效果

    Institute of Scientific and Technical Information of China (English)

    蔡莉莉

    2016-01-01

    Objective Irregular antibody screening preventive effect was observed in hemolytic transfusion reactions.Methods A retrospective analysis of November 2013 to November 2014 in our hospital 987 cases of elective surgery patients need blood transfusion clinical data to detect patients with micro-gel anti-human globulin serum irregular antibodies, the result is positive specimens for further identification, irregular antibody screening results to be analyzed.Results 987 cases of blood transfusion patients irregular antibody screening found six patients (4 males, 2 females) appeared antibody positive with probability of 0.60%, six cases of patients with irregular antibody in 2 cases of anti-E, 3 cases of anti-cE, 1 cases of anti-D, 2 patients did not last transfusion, the blood center blood transfusion with patients, 4 patients, transfusion reactions did not occur.Conclusion Irregular antibody screening for hemolytic transfusion reaction preventive effect is good, you can ensure safety of blood transfusion patients, improve the efficacy of blood transfusions for the prevention and treatment of neonatal hemolytic disease also has a positive meaning.%目的:观察不规则抗体筛查在溶血性输血反应中的预防效果。方法回顾性分析2013年11月至2014年11月在本院行择期手术987例需输血患者临床资料,使用微柱凝胶抗人球蛋白法检测患者血清不规则抗体,结果为阳性的标本进行进一步鉴定,不规则抗体筛查结果予以分析。结果对987例需输血患者进行不规则抗体筛查发现有6例患者(4例男性,2例女性)出现抗体阳性,阳性概率为0.60%,6例不规则抗体患者中有2例抗-E、3例抗-cE、1例抗-D,最后有2例患者未输血,经血液中心配合血液输血患者有4例,均没有发生输血反应。结论不规则抗体筛查对溶血性输血反应中的预防效果良好,可以确保患者输血安全,提高输血的疗效,对相关溶血病的

  11. Solution Properties of Soy Protein in DMSO/Urea Solvent System

    Institute of Scientific and Technical Information of China (English)

    XIAO Ru; YIN Duan; JIN Xin; SUN Gang

    2008-01-01

    Solution properties of 7S globulins (7S), 11S globulins (11S) and soy protein isolates (SPI) in dimethylsulfoxide ( DMSO )/urea solvent system were studied by intrinsic viscosity and particle size distributions.The results showed that the existence of urea was the main reason for the denaturation and solubility of soy protein in the system, and the effects were more obvious with increasing of urea concentration in solutions.Suitably dissolution temperature and time contributed to the solubility of soy proteins.

  12. Seasonal trends in nesting leatherback turtle (Dermochelys coriacea) serum proteins further verify capital breeding hypothesis

    Science.gov (United States)

    Perrault, Justin R.; Wyneken, Jeanette; Page-Karjian, Annie; Merrill, Anita; Miller, Debra L.

    2014-01-01

    Serum protein concentrations provide insight into the nutritional and immune status of organisms. It has been suggested that some marine turtles are capital breeders that fast during the nesting season. In this study, we documented serum proteins in neophyte and remigrant nesting leatherback sea turtles (Dermochelys coriacea). This allowed us to establish trends across the nesting season to determine whether these physiological parameters indicate if leatherbacks forage or fast while on nesting grounds. Using the biuret method and agarose gel electrophoresis, total serum protein (median = 5.0 g/dl) and protein fractions were quantified and include pre-albumin (median = 0.0 g/dl), albumin (median = 1.81 g/dl), α1-globulin (median = 0.90 g/dl), α2-globulin (median = 0.74 g/dl), total α-globulin (median = 1.64 g/dl), β-globulin (median = 0.56 g/dl), γ-globulin (median = 0.81 g/dl) and total globulin (median = 3.12 g/dl). The albumin:globulin ratio (median = 0.59) was also calculated. Confidence intervals (90%) were used to establish reference intervals. Total protein, albumin and total globulin concentrations declined in successive nesting events. Protein fractions declined at less significant rates or remained relatively constant during the nesting season. Here, we show that leatherbacks are most likely fasting during the nesting season. A minimal threshold of total serum protein concentrations of around 3.5–4.5 g/dl may physiologically signal the end of the season's nesting for individual leatherbacks. The results presented here lend further insight into the interaction between reproduction, fasting and energy reserves and will potentially improve the conservation and management of this imperiled species. PMID:27293623

  13. The anti-inflammatory and antinociceptive effects of proteins extracted from Acacia farnesiana seeds

    OpenAIRE

    L.S.S. LEAL; Silva, R.O.; T.S.L. ARAUJO; V.G. SILVA; Barbosa, A.L.R.; Medeiros, J V R; J. S. Oliveira; C.A. VENTURA

    2016-01-01

    ABSTRACT Seeds of Acacia farnesiana are commonly sold in the local markets of northeastern Brazil as a therapeutic agent. The present work aimed to evaluate the anti-inflammatory and analgesic activities of proteins obtained from A. farnesiana seeds. Five different protein fractions (albumin, globulin, prolamin, acidic and basic glutelins) were obtained and investigated for the protein pattern, the presence of hemagglutinating and proteolytic activities. The globulin fraction (GLB) was also e...

  14. Exploring variation in pea protein composition by natural selection and genetic transformation

    OpenAIRE

    Tzitzikas, E.

    2005-01-01

    Pea (Pisumsativum L.) seeds are a rich and valuable source of proteins, which can have potential for food industrial applications. Pea storage proteins are classified into two major classes: the salt-soluble globulins, and the water-soluble albumins. The globulins are subdivided into two major groups based on their sedimentation coefficient: the 11S fraction (comprising the class ofleguminwith variousisoforms) and the 7S fraction (comprising the classes ofvicilinandconvicilin, each with vario...

  15. Effects of Junk Foods on Brain Neurotransmitters (Dopamine and Serotonin) and some Biochemical Parameters in Albino Rats

    International Nuclear Information System (INIS)

    Nutritional Habits have changed significantly and junk foods have become widely popular, in recent years. The present study aimed to shed the light on the effect of potato chips and / or ketchup consumption on some biochemical parameters. Sixty four male and female albino rats were used in the study. Animals were maintained on 0.25 g potato chips/ rat and / or 0.125 g ketchup / rat, 5 days a week for 4 weeks. Potato chips showed the lowest body wt gain in the male rats after 4 weeks but, ketchup modulated this negative effect of the potato chips in the group of male animals fed on potato chips plus ketchup. Potato chips significantly decreased brain serotonin, liver glutathione (GSH) and catalase (CAT) in both sexes; brain dopamine, serum total proteins, albumin, total globulins, α2- and β1-globulins in the females and serum thyroxine (T4) in the male rats. Ketchup apparently affected serum T4 and A / G ratio in both sexes, brain dopamine and liver GSH in the males in addition to brain serotonin, serum total globulins and ?1-globulin in the female rats. Potato chips plus ketchup significantly changed T4, dopamine, GSH, CAT, α1 and α2-globulins in both sexes; serotonin and β1-globulin in the male rats, total proteins and albumin in the females. It could be concluded that potato chips consumption might induce numerous adverse effects in various body organs

  16. Serum hepatic biochemistry and electrophoretic protein profile of healthy and Ehrlich tumor-bearing mice treated with extracts of Agaricus blazei Murill

    Directory of Open Access Journals (Sweden)

    Durval Verçosa Junior

    2016-04-01

    Full Text Available Compounds isolated from Agaricus blazei Murill represent a group of promising natural immunomodulators for use in the treatment of neoplasms. We have evaluated the serum biochemical profile of healthy and Ehrlich tumor-bearing mice treated with different extracts of A. blazei. Total, supernatant, and polysaccharide extracts of A. blazei were obtained from suspensions (at acidic or neutral pH kept in a water bath at 60 °C or in an ultrasonic bath at 37 °C. After oral administering the extracts to mice for 21 days, blood samples were collected for determination of aspartate aminotransferase (AST, alanine aminotransferase (ALT, creatine kinase (CK, urea, total protein, albumin, globulins, and alpha-, beta- and gamma-globulin fractions. The presence of the tumor led to a significant increase in serum CK and AST activities and in the concentrations of total globulin and the gamma-globulin fraction, and to a decrease in the albumin and alpha2-globulin levels. The polysaccharide extracts of A. blazei reduced the serum AST and ALT activities, probably due to a hepatoprotective effect. In addition, polysaccharide and supernatant extracts inhibited the tumor-induced increase in gamma-globulin levels. Thus, the supernatant and polysaccharide fractions of the extract of A. blazei have potential for use in complementary antineoplastic treatments.

  17. O型洗涤红细胞残留ABO血型抗体检测及非O型患儿输注安全性观察%Detection of Residual ABO Antibody in Washed Red Blood Cell of Group O and Its Safety of the Application in Pediatric Patients

    Institute of Scientific and Technical Information of China (English)

    马曙轩; 宋文琪; 赵宇东; 靳剑芸; 邵华

    2013-01-01

    目的 采用多种血型血清学方法检测O型洗涤红细胞中残留ABO血型抗体(IgM抗-A,抗-B;IgG抗-A和抗-B)的含量,观察非O型患儿输注O型洗涤红细胞的安全性.方法 取O型洗涤红细胞上清,首先采用盐水试管法检测其中IgM抗-A、抗-B含量.再用凝聚胺法、微柱凝胶法、手工抗人球蛋白法检测其中IgG抗-A、抗-B含量.选取非O型患儿输注O型洗涤红细胞者(异型组)与年龄体重相当的输等量同型洗涤红细胞的患儿(同型组),分别比较2组患儿输血前后血红蛋白上升幅度、总胆红素和直接胆红素水平、直接coombs试验结果.结果 共检测O型洗涤红细胞上清液597份,盐水试管法在室温及4℃ 条件下均未观察到凝集现象,显微镜下也未观察到凝集现象.凝聚胺法、微柱凝胶法、手工抗人球蛋白法检测IgG抗-A和抗-B均为阴性.2组患儿输血前后血红蛋白上升幅度、总胆红素和直接胆红素水平差异无统计学显著性意义(t值分别为0.19,0.22和0.13,P> 0.05),直接coombs试验均为阴性.结论 血型血清学方法无法检出O型洗涤红细胞中残留ABO血型抗体.非O型患儿输注O型洗涤红细胞是安全的.%Objective To detect the residual ABO antibodies (IgM anti A and anti B,IgG anti A and anti B) in the washed group O red blood cell supernate and to observe the safety of its application in pediatric patients. Methods Immediate spin tube test was carried out to detect IgM anti A and anti B in the supernate. Polybrene,microtube gel technology and mannul anti human globulin assays were used separately to detect IgG anti A and anti B in the supernate. After receiving typed O washed red blood cell,the level of hemoglobin,content of total bilirubin and direct bilirubin and the results of direct coombs test were compared in children typed O and typed A,B or AB. Results Altogether 597 units of washed red blood cell were detected,IgM anti A and anti B were not detected by

  18. [Second structure of the protein factions from lotus seeds].

    Science.gov (United States)

    Cai, Lian-Hui; Zeng, Hong-Yan; Cai, Xi-Ling; Wang, Ya-Ju

    2011-09-01

    Following the sequential Osborne extraction procedure, the proteins of lotus seeds were classified. The secondary structures of albumin, globulin, prolamine and glutelin fractions were determined by Fourier transform infrared spectroscopy (FTIR). The FTIR images of amide I and III bands from the four protein fractions were analyzed using Fourier deconvolution and curve-fitting technique. The results showed that there were minor differences in every corresponding peak position and peak area percent of secondary structure between albumin and globulin as well as between prolamin and glutelin. But there were differences in every corresponding peak position between albumin (or globulin) and prolamin (or glutelin). Especially the area percents of the corresponding nonrandom structures (alpha-helix and beta-sheet) of albumin and globulin were significantly larger than those of prolamin and glutelin. The contents of nonrandom structures of albumin and globulin extracted with 0.1 mol x L(-1) NaCl solution were about 55% and those of prolamine and glutelin fractions were only at round 40%, indicating that the secondary structures of the salt-extraction protein were ordered and stable.

  19. Antioxidant Effect and Water-Holding Capacity of Roselle (Hibiscus sabdariffa L. Seed Protein Hydrolysates

    Directory of Open Access Journals (Sweden)

    Fatoumata Tounkara

    2013-06-01

    Full Text Available The aim of this study was to investigate the effect of in-vitro pepsin and pancreatin digestion of proteins extracted from Roselle seed on the production of bioactive peptides. Defatted Roselle seed flour was used to extract different protein fractions namely globulin, albumin and glutelin. The proteins were digested using pepsin (1 h followed by pancreatin (1 h in order to produce hydrolysates with good antioxidant activity. The prepared hydrolysates were as effective as antioxidants in model systems, in scavenging of free radicals and acting as reducing agents. This effect was concentration-dependent and was also influenced by the type of protein fraction. The albumin fraction hydrolysates prepared showed the highest antioxidant activity followed by Glutelin and Globulin hydrolysates respectively (Albumin hydrolysates>Glutelin hydrolysates>Globulin hydrolysates. All of the prepared hydrolysates were also found to be effective in enhancing water-holding capacity and cooking yield in a meat model system. Albumin hydrolysates showed the highest improved meat cooking ability followed by Glutelin and Globulin respectively (Albumin hydrolysates>Glutelin hydrolysates>Globulin hydrolysates. The molecular weight distribution analysis of the hydrolysates was determined and most of the peptides were found between 1000 Da and below. The study findings suggest that Roselle seed protein hydrolysates can be applied as functional food ingredients and that their composition determines their functional properties thus their potential application in the food and feed industries.

  20. Proteins in oats; their synthesis and changes during germination: a review.

    Science.gov (United States)

    Klose, Christina; Arendt, Elke K

    2012-01-01

    Oats (Avena sativa L.) are distinct among cereals due to their considerably higher protein concentration. At the same time oats possess a protein quality of high nutritional value and a special protein composition. Most cereals like wheat, barley, and rye have a high percentage of prolamins, the alcohol-soluble fraction, which usually contains most of the storage proteins, but oats are an exception. Their major storage proteins belong to the salt-water soluble globulin fraction, whereas oats prolamins are a minor component. During oats groat development, most obvious is the fairly linear increase in the globulin fraction. Oats globulins share structural features with the 11 S globulins of legumes and other dicots. Amino acid composition of oats is superior to that of other cereals due to the higher amount of limiting amino acids like lysine and threonine. During germination, total amino acid analysis revealed an increase in essential amino acids like lysine and tryptophan, which leads to an increased nutritional value of germinated oats. Oats protein products including globulin have been chemically modified by various methods to improve their functional properties.

  1. Anaphylaxis to buckwheat in an atopic child: a risk factor for severe allergy to nuts and seeds?

    Science.gov (United States)

    Varga, Eva-Maria; Kollmann, Dagmar; Zach, Maximilian; Bohle, Barbara

    2011-01-01

    Common buckwheat (Fagopyrum esculentum) is known to cause severe anaphylactic reactions in adult individuals. However, type I allergy to buckwheat is rarely seen in children. We report on a 7-year-old boy who developed a grade III anaphylactic reaction after consumption of a cake containing buckwheat flour. Prior to this incident, the boy had developed severe allergic reactions to hazelnuts and suffered from an oral allergy syndrome to poppy seed. Analysis of the patient's IgE reactivity by immunoblotting experiments revealed that he was sensitized to members of the 2S albumin and 11S globulin protein families in buckwheat. Additionally, cross-reactivity was found between the 11S globulins in buckwheat, poppy and hazelnut. IgE inhibition experiments indicated that the 11S globulin in buckwheat was the initial sensitizing protein. We conclude that 11S globulins in buckwheat have the potential to induce IgE antibodies cross-reactive with 11S globulins in other, botanically unrelated foods and may induce anaphylactic reactions.

  2. Affinity chromatography of human leukocyte and diploid cell interferons on sepharose-bound antibodies.

    Science.gov (United States)

    Berg, K; Ogburn, C A; Paucker, K; Mogensen, K E; Cantell, K

    1975-02-01

    Interferons produced in human peripheral leukocytes (LE) and foreskin fibroblast (FS-4) cells were subjected to affinity chromatography on Sepharose-bound globulins from rabbits immunized with these interferons. Anti-LE interferon sera neutralized both interferons, but titers against FS-4 interferon were consistently lower than those against LE interferon. Anti-FS-4 interferon sera neutralized only FS-4 but not LE interferon. Accordingly, affinity columns constructed with anti-FS-4 globulin excluded LE but not FS-4 interferon, whereas those prepared with anti-LE interferon globulin bound and eluted both LE and FS-4 interferons. Purification of native interferons of both types on anti-LE interferon-Sepharose ranged from 680- to 3,600-fold and recoveries from 72 to 126%. Specific activities of eluate pools varied from 4 to 30 times 10-6 reference (B, 69/19) units per milligram protien.

  3. Jojoba seed meal proteins associated with proteolytic and protease inhibitory activities.

    Science.gov (United States)

    Shrestha, Madan K; Peri, Irena; Smirnoff, Patricia; Birk, Yehudith; Golan-Goldhirsh, Avi

    2002-09-25

    The jojoba, Simmondsia chinensis, is a characteristic desert plant native to the Sonoran desert. The jojoba meal after oil extraction is rich in protein. The major jojoba proteins were albumins (79%) and globulins (21%), which have similar amino acid compositions and also showed a labile thrombin-inhibitory activity. SDS-PAGE showed two major proteins at 50 kDa and 25 kDa both in the albumins and in the globulins. The 25 kDa protein has trypsin- and chymotrypsin-inhibitory activities. In vitro digestibility of the globulins and albumins resembled that of casein and soybean protein concentrates and was increased after heat treatment. The increased digestibility achieved by boiling may be attributed to inactivation of the protease inhibitors and denaturation of proteins.

  4. Sex hormones in postmenopausal women with primary biliary cirrhosis

    DEFF Research Database (Denmark)

    Becker, U; Almdal, T; Christensen, E;

    1991-01-01

    To evaluate serum sex hormone profiles in nonalcoholic postmenopausal women with liver disease, 25 women with primary biliary cirrhosis (11 in cirrhotic stage) and 46 healthy controls were studied. The patients had significantly (p less than 0.05) elevated serum concentrations of estrone...... and androstenedione and significantly (p less than 0.05) lower concentrations of estrone sulfate, dehydroepiandrosterone sulfate and 5 alpha-dihydrotestosterone compared with the 46 controls. Serum concentrations of sex hormone binding globulin, testosterone, non-sex hormone binding globulin-bound testosterone...... and non-protein-bound testosterone did not differ significantly (p greater than 0.05) between primary biliary cirrhosis patients and controls. Patients in the cirrhotic stage had significantly (p less than 0.05) higher concentrations of sex hormone binding globulin than did controls. Patients...

  5. An audit of immunofixation requesting practices at a South African referral laboratory

    Directory of Open Access Journals (Sweden)

    Verena Gounden

    2014-04-01

    Full Text Available Background: It is common practice in most chemical pathology laboratories for reflective immunofixation electrophoresis (IFE to occur following the detection or suspicion of a paraprotein on serum protein electrophoresis (SPEP. The chemical pathology laboratory at Inkosi Albert Luthuli Central Hospital (IALCH in Durban, South Africa, is currently the only non-private laboratory in the KwaZulu Natal province that performs SPEP analysis, with current practice requiring that the clinician request IFE following suggestion by the laboratory after a suspicious SPEP result.Objectives: To review the current process for IFE at IALCH in the context of reflective testing and to examine the use of the alpha-2-globulin/alpha-1-globulin ratio as a predictor of a positive IFE result.Methods: Data for 1260 consecutive SPEP tests performed at the IALCH National Health Laboratory Service were collected between February and July 2011. SPEP and IFE were performed with a Sebia Hydrasys automated electrophoresis system. The alpha-2-globulin/alpha-1-globulin ratio was calculated using density of corresponding fractions on SPEP.Results: Analysis revealed that of the 1260 SPEPs performed during the analysis period, 304 IFEs were suggested by the reviewing pathologist. A total of 45 (15% of the suggested IFEs were subsequently requested by the attending clinicians. Almost half (46.5% (n = 20 of the suggested IFEs that were performed revealed the presence of a paraprotein. There was no statistically-significant difference between the alpha-2-globulin/alpha-1-globulin ratio for patients with positive or negative IFEs (p-value = 0.2.Conclusions: This study reveals the need for reflective addition of IFE testing by the laboratory following suspicious findings on SPEP.

  6. Displacement of plasma protein and conduction velocity in rats under action of acceleration forces and hypokinesia

    Science.gov (United States)

    Baranski, S.; Edelwejn, Z.; Wojtkowiak, M.

    1980-01-01

    The permeability of capillary vessels was investigated in order to determine if acceleration alone or following prolonged hypokinesia would induce changes in the vascular wall leading to the penetration by l-albumins and/or proteins with larger molecules. In rats undergoing action of +5 Gz accelerations, no increase in vascular permeability, as tested with the use of (Cr-5k)-globulin, was demostrated. In rats immobilized for 4 weeks before centrifugation, rather weak migration of (Cr-51)-globulin from the vessels was observed. Immobilization resulted also in lowering of conduction velocity in the sciatic nerve.

  7. Genetic improvement of soybean seed proteins by γ-ray irradiation

    International Nuclear Information System (INIS)

    Although soybeans have the highest protein content among seed crops, the protein quality is poor due to the low content of the sulfur-containing amino acids, cysteine and methionine. Soybean 7S globulin and 11S globulin are the two major protein components, accounting for about 70% of the total seed protein. The 11S globulin contains three to four times more methionine and cysteine per unit protein than that of the 7S globulin. Furthermore, the two globulins show considerable differences in food processing properties such as gel-making ability and emulsifying capacity. The 7S globulin is composed of three kinds of polypeptides, designated as α, α' and β subunits. A variety of soybean cv. Keburi, which lacks α' subunit was identified in a germplasm collection. An induced mutant line which lacks both α and α' subunits, was recently identified in the progeny of γ-ray-irradiated seeds from a line lacking α' subunit. On the other hand, the 11S globulin is composed of the A1aB2, A1bB1b, A2B1a, A3B4 and A4A5B3 subunits. It has become possible to breed soybeans with markedly modified protein composition from extremely high to extremely low 7S : 11S ratios using mutant genes for the subunits of the two globulins. Lipoxygenase catalyzes the hydroperoxydation of unsaturated fatty acids and polyunsaturated lipids. Soybean seeds contain three lipoxygenase isozymes, called L-1, L-2 and L-3, which are responsible for the generation of grassy-beany and bitter tastes, limiting the use of whole soybeans and soy proteins in certain food products. In the early 1980s, three types of spontaneous mutant soybean varieties lacking L-1, L-2 or L-3 were detected. Soybean cultivars having the lipoxygenase-null traits could become economically valuable for the manufacture of soy products such as soy milk due to their low levels of beany taste and their enhanced storage stability. (J.P.N.)

  8. Intestinal transport of sulfanilic acid in rats immunized with protein-sulfanilic acid conjugate.

    Science.gov (United States)

    Yamamoto, A; Kawaratani, T; Kawashima, K; Hashida, M; Sezaki, H

    1990-07-01

    Intestinal transport of sulfanilic acid was examined by means of an in vitro everted sac technique in rats immunized with a bovine gamma-globulin-sulfanilic acid conjugate. At a low concentration of sulfanilic acid, the intestinal transport of sulfanilic acid was decreased in rats immunized with bovine gamma-globulin-sulfanilic acid conjugate. This phenomenon was dose dependent and antigen specific, since there was no difference in the transport of sulfanilic acid at a high concentration and of an unrelated hapten. These results suggested that parenteral immunization impaired not only the intestinal transport of macromolecular antigens, as previously shown, but also the transport of the low molecular weight hapten, sulfanilic acid.

  9. Treatment of intractable interstitial lung injury with alemtuzumab after lung transplantation

    DEFF Research Database (Denmark)

    Kohno, M; Perch, M; Andersen, E;

    2011-01-01

    A 44-year-old woman underwent left single-lung transplantation for end-stage emphysema due to α1-antitrypsin deficiency in January 2010. Cyclosporine, azathioprine, and prednisolone were administered for immunosuppression and antithymocyte globulin for induction therapy at the time of transplanta......A 44-year-old woman underwent left single-lung transplantation for end-stage emphysema due to α1-antitrypsin deficiency in January 2010. Cyclosporine, azathioprine, and prednisolone were administered for immunosuppression and antithymocyte globulin for induction therapy at the time of...

  10. CT finding and cerebrospinal fluid proteins in muscular dystrophy patients

    International Nuclear Information System (INIS)

    We analyzed the microcomponents of protein fractions in the cerebrospinal fluid of patients with various types of muscular dystrophy. The degenerative pattern is characterized by an increase in the prealbumin and a decrease in the γ-globulin fraction is shown in the Duchenne and congenital muscular dystrophy. The increase in CSF IgG, γ-globulin fraction is shown in the myotonic dystrophy. In addition to the abnormality of IQ, EEG, and brain CT, abnormal CSF proteins obviously suggest the presence of CNS involvement in muscular dystrophy. (author)

  11. Prosopis farcta beans increase HDL cholesterol and decrease LDL cholesterol in ostriches (Struthio camelus).

    Science.gov (United States)

    Omidi, Arash; Ansari nik, Hossein; Ghazaghi, Mahmood

    2013-02-01

    Ten blue-neck male ostriches (Struthio camelus) were fed Prosopis farcta beans throughout a 30-day experiment. Blood samples were collected from ostriches on days 0 and 30 to measure levels of high-density lipoprotein (HDL) cholesterol, low-density lipoprotein (LDL) cholesterol, triglyceride, total serum protein, albumin, globulin, cholesterol, calcium, inorganic phosphorus, the activity of aspartate aminotransferase, alanine aminotransferase, and γ-glutamyl transferase (γ-GT). From days 0 to 30, HDL cholesterol, total protein, and globulins levels increased significantly whereas LDL cholesterol, inorganic phosphorus, and γ-GT activity decreased significantly.

  12. Effect of iodine-137 on sex and thyroid hormone binding to blood plasma proteins in children with functional lesions of the thyroid gland as a result of Chernobyl disaster

    International Nuclear Information System (INIS)

    In studying characteristics of specific interaction of estradiol-, testosterone- and thyriod-binding blood globulins with the corresponding ligands in children from Gomel region with with endemic swelling of the thyroid gland (degrees 1 and 2) affected by iodine-131 revealed were a reduced cooperativity in estradiol T-3 binding and a halved affinity to androgens and thyroids as compared to healthy controls. In addition, there was a drastic decline in the binding capacity of estradiol- and testosterone-binding globulins in the blood plasma. The endemic thyroid gland swelling is supposed to be due to sexual malfunction in teenagers

  13. A unified definition of clinical resistance/intolerance to hydroxyurea in essential thrombocythemia

    DEFF Research Database (Denmark)

    Barosi, G; Besses, C; Birgegard, G;

    2007-01-01

    for sodium dodecyl sulfate-polyacrylamide gel electrophoresis/immunoblotting of the three Osborne's protein fractions (albumin/globulin, gliadins and glutenins) of raw and cooked wheat. Thermal sensitivity of wheat lipid transfer protein (LTP) was investigated by spectroscopic approaches. IgE cross....../globulin fraction and several low-molecular-weight (LMW) glutenin subunits in the gluten fraction. All these allergens showed heat resistance and lack of cross-reactivity to grass pollen allergens. LTP was a major allergen only in Italian patients. CONCLUSIONS: The alpha-amylase inhibitor was confirmed...

  14. Detection of circulating antibodies against c-myc protein in cancer patient sera.

    OpenAIRE

    Ben-Mahrez, K.; Thierry, D.; Sorokine, I.; Danna-Muller, A.; Kohiyama, M

    1988-01-01

    We have partially purified an archaebacterial protein of 84 kD which shares common epitopes with the human c-myc protein as shown by its cross-reactivity with a commercialized anti-human c-myc antiserum. An antiserum raised against the 84 kD protein recognizes a 60 kD protein from HL-60 nuclei. This protein is also recognized by the anti-human c-myc antiserum. Using this archaebacterial protein as antigen for Western blot analysis, we found that the human c-myc oncogene product could be immun...

  15. Differential dissociation micromethod for the investigation of binding of metandrostenolone (Nerobol) to plasma proteins

    Energy Technology Data Exchange (ETDEWEB)

    Bojadzsieva, M.; Kocsar, L. (Orszagos Frederic Joliot-Curie Sugarbiologiai es Sugaregeszseguegyi Kutato Intezet, Budapest (Hungary)); Kremmer, T. (Orszagos Onkologiai Intezet, Budapest (Hungary))

    1985-01-01

    A micromethod was developed to determine the binding of anabolic steroids to plasma proteins. The new procedure combines precipitation with ammonium sulphate and differential dissociation. The binding parameters (association constant, specific binding capacity) are calculated on the basis of dissociation curves of sup(3)H-metandrostenolone from the precipitated sexual binding globuline.

  16. Exploring variation in pea protein composition by natural selection and genetic transformation

    NARCIS (Netherlands)

    Tzitzikas, E.

    2005-01-01

    Pea (Pisumsativum L.) seeds are a rich and valuable source of proteins, which can have potential for food industrial applications. Pea storage proteins are classified into two major classes: the salt-soluble globulins, and the water-soluble albumin

  17. High-throughput bioaffinity mass spectrometry for screening and identification of designer anabolic steroids in dietary supplements

    NARCIS (Netherlands)

    Aqai, P.; Cevik, E.; Gerssen, A.; Haasnoot, W.; Nielen, M.W.F.

    2013-01-01

    A generic high-throughput bioaffinity liquid chromatography-mass spectrometry (BioMS) approach was developed and applied for the screening and identification of known and unknown recombinant human sex hormone-binding globulin (rhSHBG)-binding designer steroids in dietary supplements. For screening,

  18. Relationships between sex hormones assessed in amniotic fluid, and maternal and umbilical cord serum: what is the best source of information to investigate the effects of fetal hormonal exposure?

    NARCIS (Netherlands)

    Beek, C.; Thijssen, J.H.; Cohen-Kettenis, P.T.; Goozen, S.H. van

    2004-01-01

    Levels of testosterone (T) (total and free), androstenedione (A4), dehydroepiandrosterone sulphate (DHEAS), sex hormone-binding globulin (SHBG), and estradiol (E2) were measured by radioimmunoassay (RIA) in 156 normal pregnancies (77 male and 79 female fetuses). Samples were obtained from amniotic f

  19. Successful outcome with a "quintuple approach" of posttransplant lymphoproliferative disorder

    NARCIS (Netherlands)

    Schaar, CG; van der Pijl, JW; van Hoek, B; de Fijter, JW; Kluin, PM; van Krieken, JHJ; Terpstra, WE; Willemze, R; Kluin-Nelemans, HC

    2001-01-01

    Background. The treatment of posttransplant lymphoproliferative disorder (PTLD) remains empirical. We review our treatment of seven cases of PTLD consisting of five interventions: 1) reduction of immunosuppression; 2) antiviral drugs; 3) interferon-alpha; 4) gamma-globulins; and 5) anti-CD19 monoclo

  20. Circadian and ultradian glucocorticoid rhythmicity: Implications for the effects of glucocorticoids on neural stem cells and adult hippocampal neurogenesis

    NARCIS (Netherlands)

    C.P. Fitzsimons; J. Herbert; M. Schouten; O.C. Meijer; P.J. Lucassen; S. Lightman

    2016-01-01

    Total glucocorticoid hormone levels in plasma of various species, including humans, follow a circadian rhythm that is made up from an underlying series of hormone pulses. In blood most of the glucocorticoid is bound to corticosteroid-binding globulin and albumin, resulting in low levels of free horm

  1. An Outbreak of Hepatitis A Among Primary and Secondary Contacts of an International Adoptee

    OpenAIRE

    Pelletier, Andrew R; Mehta, Puja J.; Burgess, Donald R.; Bondeson, Lisa M.; Carson, Patty J.; Rea, Vicki E.; Sharapov, Umid M.; Hu, Dale J.

    2010-01-01

    The Advisory Committee on Immunization Practices recommends that susceptible people traveling to developing countries receive hepatitis A vaccine or immune globulin prior to departure. Until 2009, the recommendations did not address non-traveling family members or other close contacts of international adoptees. We report an outbreak of hepatitis A in 2008 that occurred in Maine.

  2. The anti-inflammatory and antinociceptive effects of proteins extracted from Acacia farnesiana seeds

    Directory of Open Access Journals (Sweden)

    L.S.S. LEAL

    2016-03-01

    Full Text Available ABSTRACT Seeds of Acacia farnesiana are commonly sold in the local markets of northeastern Brazil as a therapeutic agent. The present work aimed to evaluate the anti-inflammatory and analgesic activities of proteins obtained from A. farnesiana seeds. Five different protein fractions (albumin, globulin, prolamin, acidic and basic glutelins were obtained and investigated for the protein pattern, the presence of hemagglutinating and proteolytic activities. The globulin fraction (GLB was also evaluated for anti-inflammatory and analgesic activities. Globulins reduced the paw edema induced by carrageenan in a dose-dependent manner, which was accompanied by a reduction of myeloperoxidase activity (p < 0.05. Additionally, GLB reduced the neutrophil peritoneal migration induced by carrageenan. However, GLB was not able to inhibit the edema triggered by dextran. Pre-treatment with globulins reduced the abdominal constrictions induced by acetic acid as well as the paw licking time induced by formalin (69.1% at first phase. However, it did not produce a significant antinociceptive effect in the hot plate test (55-56 °C. Treating the GLB with heat (at 100 °C for 30 min abolished its anti-edematogenic and hemagglutinating activities. Our results showed that seeds from A. farnesiana are a source of proteins with anti-inflammatory and analgesic properties.

  3. Anti-inflammatory activity of copper-protein complexes with reference to rheumatoid arthritis

    International Nuclear Information System (INIS)

    Work was carried out on the serum of 100 healthy subjects and hundred Rheumatoid Arthritus patients by electrophoresis and atomic absorption model Hitachi-180-80. The different proteins fractions separated by electrophoresis were subjected for estimation of Cu-Concentration. It was found that only 5% copper is present in albumin which remains constant in normal and Rheumatoid Arthritus patients. The other important group of proteins were a2-globulins, in which Cu concentration was found high in Rheumatoid Arthritus patients as compared to normal subject. There was no copper detected in beta and gamma globulins. The study can be used for diagnostic purposes. Copper complexes of alpha 2-Globulins were found anti inflammatory in nature and this quality of self defense can bring revolution in the history of all infectious diseases including Rheumatoid Arthritus simply by separating the copper complexes of alpha 2-globulins (most probably ceruloplasmin) from the blood of donors and injecting into the body of patients. It is possible by this method that the disease-Rheumatoid Arthritus may be eradicated completely. These complexes have also shown antibiotic activity in gynae patients. (author)

  4. Comparative proteome analysis of metabolic proteins from seeds of durum wheat (cv. Svevo) subjected to heat stress

    DEFF Research Database (Denmark)

    Laino, Paolo; Shelton, Dale; Finnie, Christine;

    2010-01-01

    In Central and Southern Italy, where durum wheat represents one of the most widely cultivated crops, grain filling occurs during Spring, a period characterized by sudden increases in temperature. Wheat grain proteins are classified into albumins, globulins, and prolamins. The nonprolamin fraction...

  5. Lethal factor and anti-protective antigen IgG levels associated with inhalation anthrax, Minnesota, USA.

    Science.gov (United States)

    Sprenkle, Mark D; Griffith, Jayne; Marinelli, William; Boyer, Anne E; Quinn, Conrad P; Pesik, Nicki T; Hoffmaster, Alex; Keenan, Joseph; Juni, Billie A; Blaney, David D

    2014-02-01

    Bacillus anthracis was identified in a 61-year-old man hospitalized in Minnesota, USA. Cooperation between the hospital and the state health agency enhanced prompt identification of the pathogen. Treatment comprising antimicrobial drugs, anthrax immune globulin, and pleural drainage led to full recovery; however, the role of passive immunization in anthrax treatment requires further evaluation.

  6. [Acquired agammaglobulinaemia with predominantly intestinal symptoms (author's transl)].

    Science.gov (United States)

    Goldach, R; Wittwer, J

    1977-11-01

    In a 45-year-old female patient primary acquired agammaglobulinaemia was diagnosed. Intestinal symptoms predominated. The disease was characterized by a B-cell defect. Substitution with gamma-globulin (Beriglobin) practically cured the symptoms. The pathogenesis of the disease remains unexplained. PMID:72639

  7. Impaired Reproductive Development in Sons of Women Occupationally Exposed to Pesticides during Pregnancy

    DEFF Research Database (Denmark)

    Andersen, Helle Raun; Schmidt, Ida M.; Grandjean, Philippe;

    2008-01-01

    .2-3.0) examined by the same procedure. Boys of pesticide-exposed mothers showed decreased penile length, testicular volume, serum concentrations of testosterone, and inhibin B. Serum concentrations of sex hormone-binding globulin, follicle-stimulating hormone, and the luteinizing hormone:testosterone ratio were...

  8. The Native Structure and Composition of the Cruciferin Complex in Brassica napus

    NARCIS (Netherlands)

    Nietzel, Thomas; Dudkina, Natalya V.; Haase, Christin; Denolf, Peter; Semchonok, Dmitry A.; Boekema, Egbert J.; Braun, Hans-Peter; Sunderhaus, Stephanie

    2013-01-01

    Globulins are an important group of seed storage proteins in dicotyledonous plants. They are synthesized during seed development, assembled into very compact protein complexes, and finally stored in protein storage vacuoles (PSVs). Here, we report a proteomic investigation on the native composition

  9. Lethal Factor and Anti-Protective Antigen IgG Levels Associated with Inhalation Anthrax, Minnesota, USA

    OpenAIRE

    Sprenkle, Mark D.; Griffith, Jayne; Marinelli, William; Boyer, Anne E.; Quinn, Conrad P.; Pesik, Nicki T.; Hoffmaster, Alex; Keenan, Joseph; Billie A. Juni; Blaney, David D.

    2014-01-01

    Bacillus anthracis was identified in a 61-year-old man hospitalized in Minnesota, USA. Cooperation between the hospital and the state health agency enhanced prompt identification of the pathogen. Treatment comprising antimicrobial drugs, anthrax immune globulin, and pleural drainage led to full recovery; however, the role of passive immunization in anthrax treatment requires further evaluation.

  10. Testosterone and BMD in elite male lightweight rowers

    DEFF Research Database (Denmark)

    Vinther, A; Kanstrup, I-L; Christiansen, E;

    2008-01-01

    ), free testosterone (FT), dihydrotestosterone (DHT) and sex hormone binding globulin (SHBG) and additional parameters related to bone metabolism were measured. Plasma concentrations of TT, FT and DHT were in the lower part of the normal range, while BMD was close to or above normal. BMD of total body...

  11. Testosterone and BMD in Elite Male Lightweight Rowers

    DEFF Research Database (Denmark)

    Vinther, A.; Christiansen, E.; Ekdahl, C.;

    2008-01-01

    ), free testosterone (IFT), dihydrotestosterone (DHT) and sex hormone binding globulin (SHBG) and additional parameters related to bone metabolism were measured. Plasma concentrations of TT, FT and DHT were in the lower part of the normal range, while BMD was close to or above normal. BMD of total body...

  12. Rapid determination of thyroxine binding proteins of human serum

    Directory of Open Access Journals (Sweden)

    Arima,Terukatsu

    1976-02-01

    Full Text Available A simple method is described for determing thyroxine binding proteins in human serum by electrophoresis at pH 8.6, using cellulose acetate membrane as the supporting medium. The procedure had high reliability in sera of normal subjects, pregnant women and patients with decreased thyroxine binding capacity of thyroxine binding globulin.

  13. Peripheral markers of thyroid function

    DEFF Research Database (Denmark)

    Schmidt, Ulla; Nygaard, Birte; Winther Jensen, Ebbe;

    2013-01-01

    the combination therapy. HYPOTHESIS: Peripheral tissue might also be stimulated during T4/T3 combination therapy compared with T4 monotherapy. METHODS: Serum levels of sex hormone-binding globulin (SHBG), pro-collagen-1-N-terminal peptide (PINP), and N-terminal pro-brain natriuretic peptide (NT...

  14. Traveller's hepatitis. Origin and characteristics of cases in Copenhagen 1976-1978

    DEFF Research Database (Denmark)

    Skinhøj, P; Gluud, C; Ramsøe, K

    1981-01-01

    -thirds of all patients had been on individual journeys for more than 2 weeks, mainly in Africa or Southern Asia. Areas of risk for tourist group travellers (30 cases) were established to be Northern and Central Africa. The data support the present restricted use of immune serum globulin rather than general...

  15. Prevention of spontaneous autoimmune diabetes in NOD mice by transferring in vitro antigen-pulsed syngeneic dendritic cells

    DEFF Research Database (Denmark)

    Papaccio, G; Nicoletti, F; Pisanti, F A;

    2000-01-01

    To evaluate the effect of antigen-pulsed dendritic cell (DC) transfer on the development of diabetes, 5-week-old female NOD mice received a single iv injection of splenic syngeneic DC from euglycemic NOD mice pulsed in vitro with human y globulin (HGG). Eleven of 12 mice were protected from the d...

  16. EFFECTS OF FOUR TRIHALOMETHANES ON DNA STRAND BREAKS, RENAL HYALINE DROPLET FORMATION AND SERUM TESTOSTERONE IN MALE F-344 RATS

    Science.gov (United States)

    All four possible trihalomethanes (THMs) containing bromine and chlorine, as well as perchloroethylene (PCE), were evaluated for their ability to produce DNA strand breaks, a2u-globulin rich renal deposits, and testosterone changes in male F-344 rats. Rats received daily equimola...

  17. Proteomics of desiccation tolerance during development and germination of maize embryos

    DEFF Research Database (Denmark)

    Huang, Hui; Møller, Ian Max; Song, Song-Quan

    2012-01-01

    before (28N, 52N and 72HN) and after (28D, 52D and 72HD) dehydration. Nine pre-dominantly proteins, 17.4 kDa Class I heat shock protein 3, late embryogenesis abundant protein EMB564, outer membrane protein, globulin 2, TPA:putative cystatin, NBS-LRR resistance-like protein RGC456, stress responsive...

  18. Crohn's colitis and idiopathic thrombocytopenic purpura

    OpenAIRE

    Boyne, M.; Dye, K.

    2000-01-01

    A 17 year old girl with active Crohn's colitis developed idiopathic thrombocytopenic purpura that was managed with intravenous immune globulins and cyclosporin A. The possible association between Crohn's disease and immune thrombocytopenia is explored.


Keywords: Crohn's disease; colitis; thrombocytopenia

  19. Airway cellular response to two different immunosuppressive regimens in lung transplant recipients

    NARCIS (Netherlands)

    Slebos, DJ; Kauffman, HF; Koeter, GH; Verschuuren, EAM; van der Bij, W; Postma, DS

    2005-01-01

    A number of new immunosuppressive drugs have become available in transplant medicine. We investigated the effects of two different immunosuppressive protocols on bronchoalveolar lavage fluid cellular characteristics in 34 lung transplant recipients who were treated with anti-thymocyte globulin induc

  20. Anti-HBs levels in infants of hepatitis B carrier mothers after delayed active immunization with recombinant vaccine concomitant with DTP-polio vaccine: Is there need for a second dose of HBIg?

    NARCIS (Netherlands)

    P.M. Grosheide (Pia Maria); R. Del Canho (R.); M. Voogd (M.); R.A. Heijtink; S.W. Schalm (Solko)

    1994-01-01

    textabstractThe need for an additional dose of hepatitis B immune globulin (HBIg) was studied by comparing infants receiving 1 ml HBIg at birth followed by hepatitis B immunization, concomitant with DTP-polio vaccine, at 3, 4, 5 and 11 months (schedule E), with infants receiving the same schedule wi

  1. Drug: D08789 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available D08789 Drug Freeze-dried human anti-D (Rho) immunoglobulin; Anti D globulin (TN) Th...anisms and parasites 63 Biological preparations 634 Human blood preparations 6343 Plasma preparations D08789 Freeze-dried human anti-D (Rho) immunoglobulin PubChem: 96025472 ...

  2. The glycosylation and characterization of the candidate Gc macrophage activating factor

    DEFF Research Database (Denmark)

    Ravnsborg, Tina; Olsen, Dorthe T; Thysen, Anna Hammerich;

    2010-01-01

    globulin with only a single GalNAc attached to T420. In this study we confirm the location of a linear trisaccharide on T420. Furthermore, we provide the first structural evidence of the generation of the proposed GcMAF by use of glycosidase treatment and mass spectrometry. Additionally the generated Gc...

  3. Pea proteins for piglets: effects on digestive processes.

    NARCIS (Netherlands)

    le Guen, M.P.

    1993-01-01

    White-flowered pea ( Pisum sativum ) contains 20 to 25% crude protein (Nx6.25). The pea proteins consist in globulins (60%) and albumins (25%). Because the pea albumin proteins have biological activities due to their metabolic roles in the seed, some of them are called antinutritional factors (ANFs)

  4. Disease: H00377 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available is. WHO estimates that over 30 000 people in Asia and at least 24 000 people in A...DR:D06504] Rabies immune globulin [DR:D05686] Preexposure vaccination with modern cell culture vaccine is recommended for people

  5. Gender differences in the associations between cortisol and insulin in healthy subjects

    NARCIS (Netherlands)

    Stolk, RP; Lamberts, SWJ; deJong, FH; Pols, HAP; Grobbee, DE

    1996-01-01

    To investigate the role of cortisol in the etiology of insulin resistance in men and women, we examined 218 healthy non-hospitalized elderly, selected from the Rotterdam Study. Free cortisol was assessed by the ratio of fasting serum cortisol over corticosteroid-binding globulin (CBG), and insulin r

  6. Metabolic profile of a continuous versus a cyclic low-dose combined oral contraceptive after one year of use

    NARCIS (Netherlands)

    Rad, M.; Kluft, C.; Kam, M.L. de; Meijer, P.; Cohen, A.F.; Grubb, G.S.; Constantine, G.D.; Burggraaf, J.

    2011-01-01

    Objectives?To compare the effects of a combined oral contraceptive (COC) taken continuously with those of one of similar composition taken cyclically on 30 variables related to haemostasis, lipids, carbohydrates, bone metabolism, and sex hormone-binding globulin (SHBG). Methods?Randomised, open-labe

  7. Hypothalamic-pituitary-gonadal function in relation to liver function in men with alcoholic cirrhosis

    DEFF Research Database (Denmark)

    Gluud, C; Bahnsen, M; Bennett, Patrick;

    1983-01-01

    Serum concentrations of oestrone, oestradiol, follicle-stimulating hormone (FSH), luteinizing hormone (LH), and sex hormone-binding globulin (SHBG) were significantly (P less than 0.01) raised in men with alcoholic liver cirrhosis (no. = 42) compared with age-matched controls (no. = 20). No signi...

  8. Factors influencing the adrenocorticotropin test: role of contemporary cortisol assays, body composition, and oral contraceptive agents

    DEFF Research Database (Denmark)

    Klose, Marianne; Lange, Martin; Rasmussen, Aase Krogh;

    2007-01-01

    -hormone levels; corticosteroid-binding globulin levels; and test conditions (fasting/nonfasting, rest/intermittent exercise). METHODS: A 250-microg ACTH test (0800-1000 h) was performed in 100 unmedicated subjects, 13 women taking oral contraception (OC), and six men with nephrotic syndrome. Tests were performed...

  9. Rabies Exposure: When Should I Seek Medical Attention?

    Science.gov (United States)

    ... with? What type of exposure occurred? Is the animal available for testing? The only well-documented cases of rabies caused ... rabies What type of exposure occurred? Is the animal available for testing? Medical care Human rabies immune globulin Rabies vaccine ...

  10. Seasonal changes in blood serum protein fractions and in activity of AspAT and AlAT in Arabian brood mares and their foals.

    Science.gov (United States)

    Gill, J; Jakubów, K; Kompanowska-Jezierska, E; Kott, A; Szumska, D

    1985-01-01

    In 34 pure breed Arabian horses divided into four groups (Gr. I--10 pregnant mares, Gr. II--7 barren mares, Gr. III--10 foals born in 1981, Gr. IV--7 foals born in 1982) seasonal changes in total blood serum protein, its electrophoretic fractions and the activity of AspAT and AlAT were studied. Seasonal cyclicity was found in all groups in the amount of total serum proteins, and alpha 2- and beta 1-globulin fractions. Cyclicity was found in the level of albumin and activity of AspAT in three groups, not Gr. II, and in gamma-globulin, not Gr. IV. beta 2-globulin and AlAT cyclicity was found in two groups and alpha 1-globulin cyclicity was found only in Gr. II. Out of nine indices studied, cyclicity was found in eight of them in pregnant mares, Gr. I; in seven in older foals, Gr. III; in six in the young foals, Gr. IV; and in five in barren mares, Gr. II. PMID:2864198

  11. Double-antibody radioimmunoassay for staphylococcal enterotoxin C2

    International Nuclear Information System (INIS)

    A sensitive double-antibody radioimmunoassay for staphylococcal enterotoxin C2 is described. The assay procedure employs anti-rabbit gamma globulin, prepared in goats, to precipitate the antigen-antibody complex of enterotoxin C2 and anti-enterotoxin C2. The test is sensitive to 100 pg of enterotoxin

  12. Effects of estrogen replacement and lower androgen status on skeletal muscle collagen and myofibrillar protein synthesis in postmenopausal women

    DEFF Research Database (Denmark)

    Hansen, Mette; Skovgaard, Dorthe; Reitelseder, Søren;

    2012-01-01

    myofibrillar protein FSR only in ERT users. Similarly, muscle collagen FSR tended to be lower in ERT users compared with Controls. In ERT participants, the androgen profile was reduced, whereas estradiol and sex hormone–binding globulin were higher. In conclusion, at rest, myofibrillar protein FSR was lower in...

  13. 骨髓造血细胞岛在免疫相关性血细胞减少患者中的异质性研究%The heterogeneity of hematopoietic islands in immuno-related hematocytopenia

    Institute of Scientific and Technical Information of China (English)

    孙黎飞; 韩冰; 李钢; 吴强强; 郝红峰; 明汇; 张金标

    2012-01-01

    -related pancytopenia( IRP) , study the relationship between it and MHC molecules and antihuman globulin of myeloid element (IgG) , investigate the relevant immune mechanisms and the guiding significance for clinical diagnosis and treatment. Methods ; ①Wright's Staining was utilized for observing the immunocyte quantity and hematopoietic cell component in myeloid element islands of 37 patients with IRH/IRP;②Cytochemical staining was utilized for observing the peroxidase (POX) activity and the condition of iron storage of immune cells in HI;③Immunochemistry staining was utilized for observing the MHC II (HLA-DR antigen) of antigen presenting cell in the bone marrow;④Immunofiuorescence (IF) staining was utilized for observing anti-human IgG antibody in hematopoietic cells and immune cells;⑤Observing different types of HI in the bone marrow. Results:①The hematopoietic cells and immune cells were various in HI,and were closely related with ESR, IgG, serum HLA-B27, peroxidase ( POX) , HLA-DR and IF. ②According to the biological properties of immune cells,the HI were divided into five types;antibody dependent cell cytotoxicity ( ADCC) type, macrophages (Mψ) type, antigen presenting type, plasma cells type and promyelocytic type. ③Antihuman IgG fluorescent antibodies were expressed in all naive hematopoietic cell membranes of HI,and different strong positive IF particles were present in the contact position between immune cells and hematopoietic cells and the cytoplasms of antigen presenting cells ( APC) ;④Mψ-type HI was the most in number, and was often coexisted with ADCC-type HI in patients with higher erythrocyte sedimentation rate(ESR). In the bone marrow of patients with both HLA-B27 and ESR increase, ADCC-type HI, Mψ-type HI and AP-type HI could been seen at the same time. Conclusions: The basis of HI is that the change of immature hematopoietic cell surface of patients with IRH/IRP results in antihuman IgG antibodies generation. The heterogeneity of HI is because

  14. Desempenho da tecnologia dos eritrócitos magnetizados (E.M.® Technology nos testes imunoematológicos Erythrocytes magnetized technology (E.M.® Technology performance in immunohematology assays

    Directory of Open Access Journals (Sweden)

    Shirley L. Castilho

    2008-10-01

    microplate contains monoclonal IgM antibodies. The wells of the microplate to perform antibody screening and cross-matching are coated with murine monoclonal anti-human globulin: anti-IgG for antibody screening and anti-IgG + anti-IgM for cross-matching. The E.M.® Technology is only able to detect IgG antibodies. A high-density medium layer avoids the necessity of rinsing before the antiglobulin reagent. To evaluate the performance of E.M.® Technology on a Freelys® Nano work station for blood grouping, Rh/K phenotyping, antibody screening and cross-matching, we selected samples from patients, blood donors, blood cords, red blood cells from patients with positive direct antiglobulin test (DAT and red blood cells from storage blood bags. In parallel, the tests were performed using the gel-technique (Diamed and Grifols. The concordance between E.M.® Technology and gel-test was 100% for blood grouping and Rh/K phenotyping, 94.6% for antibody screening and 92.3% for cross-matching. The sensitivity to detect IgG antibodies was 95.5% in both methods. The E.M.® Technology on a Freelys® Nano workstation provided good results in all the tests performed.

  15. HEMATOPOIETIC SYSTEM

    Institute of Scientific and Technical Information of China (English)

    2003-01-01

    12.1 Erythrocyte function2003458 A preliminary experience of treatment of severe aplastic anemia with marine anti-human CD3T lymphocyte monoclonal antibody. GUO Chengshan (郭成山), et al. Dept Hematol, 2nd Hosp, Shandong Univ, Jinan 250033. Chin J Intern Med 2003; 42(9): 632 -635.

  16. Targeting Membrane-Bound Viral RNA Synthesis Reveals Potent Inhibition of Diverse Coronaviruses Including the Middle East Respiratory Syndrome Virus

    NARCIS (Netherlands)

    Lundin, A.; Dijkman, R.; Bergstrom, T.; Kann, N.; Adamiak, B.; Hannoun, C.; Kindler, E.; Jonsdottir, H.R.; Muth, D.; Kint, J.; Forlenza, M.

    2014-01-01

    Coronaviruses raise serious concerns as emerging zoonotic viruses without specific antiviral drugs available. Here we screened a collection of 16671 diverse compounds for anti-human coronavirus 229E activity and identified an inhibitor, designated K22, that specifically targets membrane-bound corona

  17. Bicyclams, selective antagonists of the human chemokine receptor CXCR4, potently inhibit feline immunodeficiency virus replication

    NARCIS (Netherlands)

    Horzinek, M.C.; Egberink, H.F.; Clercq, E. de; Vliet, A.L.W. van; Balzarini, J.; Bridger, G.J.; Henson, G.; Schols, D.

    1999-01-01

    Bicyclams are low-molecular-weight anti-human immunodeficiency virus (HIV) agents that have been shown to act as potent and selective CXC chemokine receptor 4 (CXCR4) antagonists. Here, we demonstrate that bicyclams are potent inhibitors of feline immunodeficiency virus (FIV) replication when evalua

  18. Detection and partial characterization of a midlamina lucida-hemidesmosome-associated antigen (19-DEJ-1) present within human skin

    DEFF Research Database (Denmark)

    Fine, J D; Horiguchi, Y; Jester, J;

    1989-01-01

    A murine anti-human monoclonal antibody (19-DEJ-1) has been produced that binds to basement membranes (BMs) of the dermoepidermal junction and arrector pili muscles but not to either dermal glandular or vascular BMs. 19-DEJ-1 also recognizes BMs underneath epithelia of buccal mucosa, tongue...

  19. Pharmacokinetics and anti-HIV-1 efficacy of negatively charged human serum albumins in mice

    NARCIS (Netherlands)

    Kuipers, M.E; Swart, P.J; Schutten, M; Smit, C; Proost, Hans; Osterhaus, A.DME; Meijer, D.K F

    1997-01-01

    Negatively charged albumins (NCAs, with the prototypes succinylated human serum albumin (Suc-HSA) and aconitylated human serum albumin (Aco-HSA)), modified proteins with a potent anti-human immunodeficiency virus type 1 (anti-HIV-1) activity in vitro, were studied for their pharmacokinetic behaviour

  20. Development and Characterization of Clinical-Grade Zr-89-Trastuzumab for HER2/neu ImmunoPET Imaging

    NARCIS (Netherlands)

    Dijkers, Eli C. F.; Kosterink, Jos G. W.; Rademaker, Anna P.; Perk, Lars R.; van Dongen, Guus A. M. S.; Bart, Joost; de Jong, Johan R.; de Vries, Elisabeth G. E.; Lub-de Hooge, Marjolijn N.

    2009-01-01

    The anti-human epidermal growth factor receptor 2 (HER2/neu) antibody trastuzumab is administered to patients with HER2/neu-overexpressing breast cancer. Whole-body noninvasive HER2/neu scintigraphy could help to assess and quantify the HER2/neu expression of all lesions, including nonaccessible met