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Sample records for anti-aging hormetic effects

  1. Hormetic effect induced by depleted uranium in zebrafish embryos.

    Science.gov (United States)

    Ng, C Y P; Cheng, S H; Yu, K N

    2016-06-01

    The present work studied the hormetic effect induced by uranium (U) in embryos of zebrafish (Danio rerio) using apoptosis as the biological endpoint. Hormetic effect is characterized by biphasic dose-response relationships showing a low-dose stimulation and a high-dose inhibition. Embryos were dechorionated at 4h post fertilization (hpf), and were then exposed to 10 or 100μg/l depleted uranium (DU) in uranyl acetate solutions from 5 to 6 hpf. For exposures to 10μg/l DU, the amounts of apoptotic signals in the embryos were significantly increased at 20 hpf but were significantly decreased at 24 hpf, which demonstrated the presence of U-induced hormesis. For exposures to 100μg/l DU, the amounts of apoptotic signals in the embryos were significantly increased at 20, 24 and 30 hpf. Hormetic effect was not shown but its occurrence between 30 and 48 hpf could not be ruled out. In conclusion, hormetic effect could be induced in zebrafish embryos in a concentration- and time-dependent manner.

  2. Molecular mechanisms of anti-aging hormetic effects of mild heat stress on human cells

    DEFF Research Database (Denmark)

    Rattan, Suresh I S; Eskildsen-Helmond, Yvonne E G; Beedholm, Rasmus

    2004-01-01

    at the levels of maintenance of stress protein profile; reduction in the accumulation of oxidatively and glycoxidatively damaged proteins; stimulation of the proteasomal activities for the degradation of abnormal proteins; improved cellular resistance to ethanol, hydrogenperoxide, and ultraviolet-B rays......; and enhanced levels of various antioxidant enzymes. We are now undertaking a detailed analysis of the signal transduction pathways to determine alterations in the phosphorylation and dephosphorylation states of extracellular signal-related kinase, c-Jun terminal kinase and p38 MAP-kinases as a measure...

  3. Study of the hormetic effect of disinfectants chlorhexidine, povidone iodine and benzalkonium chloride.

    Science.gov (United States)

    Morales-Fernández, L; Fernández-Crehuet, M; Espigares, M; Moreno, E; Espigares, E

    2014-01-01

    The study of the dose-response relationship of disinfectants is of great importance in treating infection, the objective being to use concentrations above the minimal bactericidal concentration (MBC). Below these concentrations, the bacteriostatic or bactericidal effect may be insufficient. Moreover, at low concentrations, a hormetic effect may be observed, producing a stimulation of growth instead of inhibitory action. Hormesis is not well known in the context of antimicrobial substances. This study investigates the possible existence of a hormetic effect in three commonly used antiseptics-chlorhexidine digluconate, povidone iodine and benzalkonium chloride-on strains of reference of Staphylococcus aureus and Pseudomonas aeruginosa. Growth curves were determined for different concentrations of the disinfectants. The variables studied-concentration of disinfectant, instantaneous growth rate and number of generations-were analysed using linear, quadratic and cubic models to adjust for the variables. The three disinfectants tested show a significant hormetic effect with P. aeruginosa and a less significant effect with S. aureus. These findings point to a dose-response effect that is not linear at low concentrations, while hormetic effects observed at some low concentrations result in greater bacterial growth. In infected wounds, materials or surfaces where microorganisms may occupy zones of difficult access for a disinfectant, the hormetic effect may have important consequences.

  4. Hormetic Effect of Berberine Attenuates the Anticancer Activity of Chemotherapeutic Agents.

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    Bao, Jiaolin; Huang, Borong; Zou, Lidi; Chen, Shenghui; Zhang, Chao; Zhang, Yulin; Chen, Meiwan; Wan, Jian-Bo; Su, Huanxing; Wang, Yitao; He, Chengwei

    2015-01-01

    Hormesis is a phenomenon of biphasic dose response characterized by exhibiting stimulatory or beneficial effects at low doses and inhibitory or toxic effects at high doses. Increasing numbers of chemicals of various types have been shown to induce apparent hormetic effect on cancer cells. However, the underlying significance and mechanisms remain to be elucidated. Berberine, one of the major active components of Rhizoma coptidis, has been manifested with notable anticancer activities. This study aims to investigate the hormetic effect of berberine and its influence on the anticancer activities of chemotherapeutic agents. Our results demonstrated that berberine at low dose range (1.25 ~ 5 μM) promoted cell proliferation to 112% ~170% of the untreated control in various cancer cells, while berberine at high dose rage (10 ~ 80 μM) inhibited cell proliferation. Further, we observed that co-treatment with low dose berberine could significantly attenuate the anticancer activity of chemotherapeutic agents, including fluorouracil (5-FU), camptothecin (CPT), and paclitaxel (TAX). The hormetic effect and thereby the attenuated anticancer activity of chemotherapeutic drugs by berberine may attributable to the activated protective stress response in cancer cells triggered by berberine, as evidenced by up-regulated MAPK/ERK1/2 and PI3K/AKT signaling pathways. These results provided important information to understand the potential side effects of hormesis, and suggested cautious application of natural compounds and relevant herbs in adjuvant treatment of cancer.

  5. Hormetic Effect of Berberine Attenuates the Anticancer Activity of Chemotherapeutic Agents.

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    Jiaolin Bao

    Full Text Available Hormesis is a phenomenon of biphasic dose response characterized by exhibiting stimulatory or beneficial effects at low doses and inhibitory or toxic effects at high doses. Increasing numbers of chemicals of various types have been shown to induce apparent hormetic effect on cancer cells. However, the underlying significance and mechanisms remain to be elucidated. Berberine, one of the major active components of Rhizoma coptidis, has been manifested with notable anticancer activities. This study aims to investigate the hormetic effect of berberine and its influence on the anticancer activities of chemotherapeutic agents. Our results demonstrated that berberine at low dose range (1.25 ~ 5 μM promoted cell proliferation to 112% ~170% of the untreated control in various cancer cells, while berberine at high dose rage (10 ~ 80 μM inhibited cell proliferation. Further, we observed that co-treatment with low dose berberine could significantly attenuate the anticancer activity of chemotherapeutic agents, including fluorouracil (5-FU, camptothecin (CPT, and paclitaxel (TAX. The hormetic effect and thereby the attenuated anticancer activity of chemotherapeutic drugs by berberine may attributable to the activated protective stress response in cancer cells triggered by berberine, as evidenced by up-regulated MAPK/ERK1/2 and PI3K/AKT signaling pathways. These results provided important information to understand the potential side effects of hormesis, and suggested cautious application of natural compounds and relevant herbs in adjuvant treatment of cancer.

  6. Role of antioxidants in the skin: anti-aging effects.

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    Masaki, Hitoshi

    2010-05-01

    Intracellular and extracellular oxidative stress initiated by reactive oxygen species (ROS) advance skin aging, which is characterized by wrinkles and atypical pigmentation. Because UV enhances ROS generation in cells, skin aging is usually discussed in relation to UV exposure. The use of antioxidants is an effective approach to prevent symptoms related to photo-induced aging of the skin. In this review, the mechanisms of ROS generation and ROS elimination in the body are summarized. The effects of ROS generated in the skin and the roles of ROS in altering the skin are also discussed. In addition, the effects of representative antioxidants on the skin are summarized with a focus on skin aging.

  7. Low doses of nanodiamonds and silica nanoparticles have beneficial hormetic effects in normal human skin fibroblasts in culture

    DEFF Research Database (Denmark)

    Mytych, Jennifer; Wnuk, Maciej; Rattan, Suresh

    2016-01-01

    oxygenease HO-1), sirtuin (SIRT1), and DNA methyltransferase II (DNMT2). These results imply that ND and SiO2-NP at low doses are potential hormetins, which exert mild stress-induced beneficial hormetic effects through improved survival, longevity, maintenance, repair and function of human cells...

  8. Hormetic effect of amyloid-beta peptide in hippocampal synaptic plasticity and memory

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    Daniela Puzzo

    2012-09-01

    Full Text Available Background: The term hormesis refers to a biphasic dose-response phenomenon characterized by low-dose stimulation and high-dose inhibition represented by a J-shaped or U-shaped curve, depending on the parameter measured (Calabrese and Baldwin, Hum Exp Toxicol, 2002. Indeed, several, if not all, physiological molecules (i.e. glutamate, glucocorticoids, nitric oxide are likely to present a hormetic effect, exhibiting opposite effects at high or low concentrations. In the last few years, we have focused on amyloid-beta (A, a peptide widely known because it is produced in high amounts during Alzheimer’s disease (AD. A is considered a toxic fragment causing synaptic dysfunction and memory impairment (Selkoe, Science, 2002. However, the peptide is normally produced in the healthy brain and growing evidences indicate that it might have a physiologic function. Aim: Based on previous results showing that picomolar concentrations of A42 enhance synaptic plasticity and memory (Puzzo et al, J Neurosci, 2008 and that endogenous A is necessary for synaptic plasticity and memory (Puzzo et al, Ann Neurol, 2011, the aim of our study was to demonstrate the hormetic role of A in synaptic plasticity and memory. Methods: We used 3-month old wild type mice to analyze how synaptic plasticity, measured on hippocampal slices in vitro, and spatial reference memory were modified by treatment with different doses of A (from 2 pM to 20 μM. Results: We demonstrated that A has a hormetic effect (Puzzo et al, Neurobiol Aging, 2012 with low-doses (200 pM stimulating synaptic plasticity and memory and high-doses (≥ 200 nM inhibiting these processes. Conclusions: Our results suggest that, paradoxically, very low doses of A might serve to enhance memory at appropriate concentrations and conditions. These findings raise several issues when designing

  9. Anti-Aging Effects of Some Selected Iranian Folk Medicinal Herbs-Biochemical Evidences

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    Azadeh Mohammadirad

    2013-11-01

    These data for the first time indicate significant anti-aging potential of examined herbs. Results showed that D-galactose induces a significant oxidative stress and promotes proinflammatory cascade of aging while all herbs more or less recovered these changes. Among 9 herbal extracts, Silybum marianum showed the best effect in restoring aging changes.

  10. Beneficial effects of mild stress (hormetic effects): dietary restriction and health.

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    Kouda, Katsuyasu; Iki, Masayuki

    2010-01-01

    Hormesis is defined as a dose-response phenomenon characterized by low-dose stimulation and high-dose inhibition, and has been recognized as representing an overcompensation for mild environmental stress. The beneficial effects of mild stress on aging and longevity have been studied for many years. In experimental animals, mild dietary stress (dietary restriction, DR) without malnutrition delays most age-related physiological changes, and extends maximum and average lifespan. Animal studies have also demonstrated that DR can prevent or lessen the severity of cancer, stroke, coronary heart disease, autoimmune disease, allergy, Parkinson's disease and Alzheimer's disease. The effects of DR are considered to result from hormetic mechanisms. These effects were reported by means of various DR regimens, such as caloric restriction, total-nutrient restriction, alternate-day fasting, and short-term fasting. Mild dietary stress, including restriction of amount or frequency of intake, is the essence of DR. For more than 99% of their history, humans lived as hunter-gatherers and adapted to restrictions in their food supply. On the other hand, an oversufficiency of food for many today has resulted in the current global epidemic of obesity and obesity-related diseases. DR may be used, therefore, as a novel approach for therapeutic intervention in several diseases, when detailed information about effects of mild dietary stress on human health is obtained from clinical trials.

  11. Sterols from Mytilidae show anti-aging and neuroprotective effects via anti-oxidative activity.

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    Sun, Yujuan; Lin, Yanfei; Cao, Xueli; Xiang, Lan; Qi, Jianhua

    2014-11-25

    For screening anti-aging samples from marine natural products, K6001 yeast strain was employed as a bioassay system. The active mussel extract was separated to give an active sterol fraction (SF). SF was further purified, and four sterol compounds were obtained. Their structures were determined to be cholesterol (CHOL), brassicasterol, crinosterol, and 24-methylenecholesterol. All compounds showed similar anti-aging activity. To understand the action mechanism involved, anti-oxidative experiments, reactive oxygen species (ROS) assays, and malondialdehyde (MDA) tests were performed on the most abundant compound, CHOL. Results indicated that treatment with CHOL increases the survival rate of yeast under oxidative stress and decreases ROS and MDA levels. In addition, mutations of uth1, skn7, sod1, and sod2, which feature a K6001 background, were employed and the lifespans of the mutations were not affected by CHOL. These results demonstrate that CHOL exerts anti-aging effects via anti-oxidative stress. Based on the connection between neuroprotection and anti-aging, neuroprotective experiments were performed in PC12 cells. Paraquat was used to induce oxidative stress and the results showed that the CHOL and SF protect the PC12 cells from the injury induced by paraquat. In addition, these substance exhibited nerve growth factor (NGF) mimic activities again confirmed their neuroprotective function.

  12. An Experimental Research into the Anti-aging Effects of Radix Arctii Lappae

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    Purpose: To delve into the anti-aging effects and mechanism of Niubanggen (牛蒡根Radix Arctii Lappae). Method: The activity of SOD and the content of MDA and lipofuscin in the tissues of the liver,brain and blood serum of the lab rats were observed 30 days after they had been fed with the Niubanggen decoction. Result: The activity of SOD in the liver tissue and blood serum of the decoction-fed lab rats was improved dramatically (P<0.05 or P<0.01), the content of MDA in the brain tissue and blood serum lowered obviously (P<0.05 or P<0.01), and the content of lipofuscin dropped distinctly (P<0.01). Conclusion: The mechanism of the anti-aging effects of the Niubanggen is mainly obtained by raising the activity of SOD and reducing the contents of MDA and lipofuscin.

  13. Hormetic Effect of Chronic Hypergravity in a Mouse Model of Allergic Asthma and Rhinitis

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    Jang, Tae Young; Jung, Ah-Yeoun; Kim, Young Hyo

    2016-06-01

    We aimed to evaluate the effect of chronic hypergravity in a mouse model of allergic asthma and rhinitis. Forty BALB/c mice were divided as: group A (n = 10, control) sensitized and challenged with saline, group B (n = 10, asthma) challenged by intraperitoneal and intranasal ovalbumin (OVA) to induce allergic asthma and rhinitis, and groups C (n = 10, asthma/rotatory control) and D (n = 10, asthma/hypergravity) exposed to 4 weeks of rotation with normogravity (1G) or hypergravity (5G) during induction of asthma/rhinitis. Group D showed significantly decreased eosinophils, neutrophils, and lymphocytes in their BAL fluid compared with groups B and C (p lung homogenate, the expression of IL-1β was significantly upregulated (p lung parenchyma and turbinate, and the thickness of respiratory epithelium was significantly reduced in group D (p < 0.05). The expression of Bcl-2 and heme oxygenase-1 were significantly downregulated, Bax and extracellular dismutase significantly upregulated in Group D. Therefore, chronic hypergravity could have a hormetic effect for allergic asthma and rhinitis via regulation of genes involved in antioxidative and proapoptotic pathways. It is possible that we could use hypergravity machinery for treating allergic respiratory disorders.

  14. A study of Lycium barbarum polysaccharides (LBP) extraction technology and its anti-aging effect.

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    Yi, Ran; Liu, Xiao-Min; Dong, Qi

    2013-01-01

    The objective of the study was to optimise the LBP extraction technology and to study the anti-aging effect of LBP by establishing D-gal aging mouse model. Orthogonal design was used to study the extraction technology. The experimental aging mouse model was formed by continuous injection of D-gal, and the anti-aging capacity of LBP was tested using measuring MDA, CAT and GSH-px contents and SOD activity in blood and SOD, MDA and Hyp levels in skin. The results showed that the optimum LBP extraction option determined by the orthogonal design is as follows: solid-liquid ratio of 1:30, extraction for 2 times, 90 min each time, and power is 100 kHz. Thus, LBP can increase SOD, CAT and GSH-px levels in blood and reduce MDA level. It can also improve skin SOD activity, reduce skin MDA content, and increase Hyp content. We concluded that the extraction method established in this experiment is easy and feasible, and the yield of LBP is high, apparently showing that LBP has the potential of delaying senility in D-gal induced mice.

  15. Mechanisms underlying the anti-aging and anti-tumor effects of lithocholic bile acid.

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    Arlia-Ciommo, Anthony; Piano, Amanda; Svistkova, Veronika; Mohtashami, Sadaf; Titorenko, Vladimir I

    2014-09-18

    Bile acids are cholesterol-derived bioactive lipids that play essential roles in the maintenance of a heathy lifespan. These amphipathic molecules with detergent-like properties display numerous beneficial effects on various longevity- and healthspan-promoting processes in evolutionarily distant organisms. Recent studies revealed that lithocholic bile acid not only causes a considerable lifespan extension in yeast, but also exhibits a substantial cytotoxic effect in cultured cancer cells derived from different tissues and organisms. The molecular and cellular mechanisms underlying the robust anti-aging and anti-tumor effects of lithocholic acid have emerged. This review summarizes the current knowledge of these mechanisms, outlines the most important unanswered questions and suggests directions for future research.

  16. Anti-aging effects of vitamin C on human pluripotent stem cell-derived cardiomyocytes.

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    Kim, Yoon Young; Ku, Seung-Yup; Huh, Yul; Liu, Hung-Ching; Kim, Seok Hyun; Choi, Young Min; Moon, Shin Yong

    2013-10-01

    Human pluripotent stem cells (hPSCs) have arisen as a source of cells for biomedical research due to their developmental potential. Stem cells possess the promise of providing clinicians with novel treatments for disease as well as allowing researchers to generate human-specific cellular metabolism models. Aging is a natural process of living organisms, yet aging in human heart cells is difficult to study due to the ethical considerations regarding human experimentation as well as a current lack of alternative experimental models. hPSC-derived cardiomyocytes (CMs) bear a resemblance to human cardiac cells and thus hPSC-derived CMs are considered to be a viable alternative model to study human heart cell aging. In this study, we used hPSC-derived CMs as an in vitro aging model. We generated cardiomyocytes from hPSCs and demonstrated the process of aging in both human embryonic stem cell (hESC)- and induced pluripotent stem cell (hiPSC)-derived CMs. Aging in hESC-derived CMs correlated with reduced membrane potential in mitochondria, the accumulation of lipofuscin, a slower beating pattern, and the downregulation of human telomerase RNA (hTR) and cell cycle regulating genes. Interestingly, the expression of hTR in hiPSC-derived CMs was not significantly downregulated, unlike in hESC-derived CMs. In order to delay aging, vitamin C was added to the cultured CMs. When cells were treated with 100 μM of vitamin C for 48 h, anti-aging effects, specifically on the expression of telomere-related genes and their functionality in aging cells, were observed. Taken together, these results suggest that hPSC-derived CMs can be used as a unique human cardiomyocyte aging model in vitro and that vitamin C shows anti-aging effects in this model.

  17. Anti-aging and anti-microbial effects of melleolide on various types of yeast.

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    Nakaya, Shigeru; Kobori, Hajime; Sekiya, Atsushi; Kawagishi, Hirokazu; Ushimaru, Takashi

    2014-01-01

    The chronological lifespan (CLS) of the budding yeast Saccharomyces cerevisiae is a model for the aging of post-mitotic cells in higher eukaryotes. In this study, we found that the sesquiterpene aryl ester melleolide expands the CLS of budding yeast. In contrast, melleolide compromised the CLS of the fission yeast Schizosaccharomyces pombe. This indicates that melleolide might have a potential anti-aging activity against some types of cell, and that it might be useful as a selective anti-fungal drug.

  18. Anti-Aging Effect of Siraitia grosuenorii by Enhancement of Hematopoietic Stem Cell Function.

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    Bai, Lin; Shi, Guiying; Yang, Yajun; Chen, Wei; Zhang, Lianfeng

    2016-01-01

    Anti-aging has always been a popular topic, and there are many claims about the existence of factors that can slow, stop, or even reverse the aging process. Siraitia grosuenorii, a local fruit in china, has been used for the treatment of gastritis, sore throats, and whooping cough in traditional Chinese medicine. The individuals who took the juice of Siraitia grosuenorii regularly had increased longevity in the Guangxi Province, which is located in the Southern part of China. In this paper, we fed mice with Siraitia grosuenorii for 10 months to identify the role of Siraitia grosuenorii in anti-aging and to investigate its corresponding mechanism. The results showed that mice fed with Siraitia grosuenorii displayed a slower aging process. The extension of the aging process was due to the enhanced function of HSCs. FACS analysis showed that the number of LSKs, LT-HSCs, ST-HSCs and MPPs from Siraitia grosuenorii mice was decreased. In vitro, a clonigenic assay showed that LT-HSCs from Siraitia grosuenorii mice increased the ability of self-renewal. Moreover, Siraitia grosuenorii mice maintained the quiescence of LSKs, decreased the level of ROS and reduced the amount of senescence associated β-gal positive cells. Furthermore, Siraitia grosuenorii mice decreased the expression of senescence-associated proteins. Siraitia grosuenorii maintained quiescence, decreased senescence and enhanced the function of HSCs, slowing the aging process of mice.

  19. Low doses of nanodiamonds and silica nanoparticles have beneficial hormetic effects in normal human skin fibroblasts in culture.

    Science.gov (United States)

    Mytych, Jennifer; Wnuk, Maciej; Rattan, Suresh I S

    2016-04-01

    Nanodiamonds (ND) and silica nanoparticles (SiO2-NP) have been much investigated for their toxicity at high doses, little is known about their biological activity at low concentrations. Here we report the biphasic dose response of ND and SiO2-NP in modulating normal human facial skin fibroblasts (FSF1) in culture. ND and SiO2-NP at low concentration (up to 0.5 μg/ml) had beneficial effects on FSF1 in terms of increasing their proliferation and metabolic activity. Exposure of FSF1 cells to low levels of NP enhanced their wound healing ability in vitro and slowed down aging during serial passaging as measured by maintenance of youthful morphology, reduction in the rate of loss of telomeres, and the over all proliferative characteristics. Furthermore, NP treatment induced the activation of Nrf2- and FOXO3A-mediated cellular stress responses, including an increased expression of heme oxygenease (HO-1), sirtuin (SIRT1), and DNA methyltransferase II (DNMT2). These results imply that ND and SiO2-NP at low doses are potential hormetins, which exert mild stress-induced beneficial hormetic effects through improved survival, longevity, maintenance, repair and function of human cells.

  20. Anti-Aging Effects of the Hanwoo Leg Bone, Foot and Tail Infusions (HLI, HFI and HTI) on Skin Fibroblast.

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    Seol, Ja Young; Yoon, Ji Young; Jeong, Hee Sun; Joo, Nami; Choi, Soon Young

    2016-01-01

    Many researchers revealed that collagen contribute to maintaining the skin's elasticity and inhibit wrinkling of skin. Korean native cattle (Hanwoo) bone (leg bone, foot and tail) infusion contains the various inorganic materials, collagen and chondroitin sulfate. All of this, a large quantity of collagen is included in Hanwoo infusion. Therefore, this study emphasized on the effects of collagen in the Hanwoo bone infusion. For the first time, Hanwoo bone infusions were directly added to the media of Human Dermal Fibroblast (NHDF-c) to test anti-aging effects. First, it was identified that growth rate of skin fibroblast was increased. Furthermore, the Hanwoo bone infusion increased a 50% of fibroblast collagen synthesis. Also, suppression of skin fibroblast aging was confirmed by treatment Hanwoo bone infusion. In conclusion, this study demonstrates the effects of infusion made from Hanwoo leg bone, foot and tail on anti-aging, wrinkle inhibiting and skin fibroblast elasticity maintaining. Therefore, this study identified that traditional infusion has effects that are good for skin elasticity.

  1. Anti-Aging Effects of the Hanwoo Leg Bone, Foot and Tail Infusions (HLI, HFI and HTI) on Skin Fibroblast

    Science.gov (United States)

    Yoon, Ji Young; Jeong, Hee Sun; Joo, Nami

    2016-01-01

    Many researchers revealed that collagen contribute to maintaining the skin’s elasticity and inhibit wrinkling of skin. Korean native cattle (Hanwoo) bone (leg bone, foot and tail) infusion contains the various inorganic materials, collagen and chondroitin sulfate. All of this, a large quantity of collagen is included in Hanwoo infusion. Therefore, this study emphasized on the effects of collagen in the Hanwoo bone infusion. For the first time, Hanwoo bone infusions were directly added to the media of Human Dermal Fibroblast (NHDF-c) to test anti-aging effects. First, it was identified that growth rate of skin fibroblast was increased. Furthermore, the Hanwoo bone infusion increased a 50% of fibroblast collagen synthesis. Also, suppression of skin fibroblast aging was confirmed by treatment Hanwoo bone infusion. In conclusion, this study demonstrates the effects of infusion made from Hanwoo leg bone, foot and tail on anti-aging, wrinkle inhibiting and skin fibroblast elasticity maintaining. Therefore, this study identified that traditional infusion has effects that are good for skin elasticity. PMID:27194933

  2. Dual mTORC1/C2 inhibitors: gerosuppressors with potential anti-aging effect.

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    Sousa-Victor, Pedro; García-Prat, Laura; Muñoz-Cánoves, Pura

    2015-09-15

    Over the past decade, our understanding of the molecular and cellular mechanisms presiding over cellular and tissue decline with aging has greatly advanced. Classical hallmarks of aging cell include increasing levels of reactive oxygen species, DNA damage and senescence entry, which disrupt tissue architecture and function. Tissue dysfunction with aging has been shown to correlate with a cellular switch from a G0 reversible quiescence state into a G0 irreversible senescence state (geroconversion), causing a permanent proliferative block. The TOR (target of rapamycin) kinase has been shown to promote geroconversion. Rapamycin and other rapalogs specifically suppress activity of the mammalian TOR (mTOR) complex 1 (mTORC1) -but not mTOR complex 2 (mTORC2)- and decrease senescence entry, thus preserving proliferative potential. In this perspective, we briefly comment recent progress of Leontieva and colleagues showing a new class of non-rapalog drugs that target simultaneously mTORC1 and mTORC2 and prevent geroconversion in a more efficient way than rapamycin. Its potential future use as rejuvenating, anti-aging therapeutics is therefore proposed.

  3. Pathogen-induced Caenorhabditis elegans developmental plasticity has a hormetic effect on the resistance to biotic and abiotic stresses

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    Leroy Magali

    2012-09-01

    on pathogens has a hormetic effect on adult nematodes, as it results in increased resistance to different pathogens and to heat shock. Such developmental plasticity of C. elegans nematodes, which are self-fertilizing homozygous animals producing offspring with negligible genetic variation, could increase the probability of survival in changing environments.

  4. Dissection of the hormetic curve: analysis of components and mechanisms.

    Science.gov (United States)

    Lushchak, Volodymyr I

    2014-07-01

    The relationship between the dose of an effector and the biological response frequently is not described by a linear function and, moreover, in some cases the dose-response relationship may change from positive/adverse to adverse/positive with increasing dose. This complicated relationship is called "hormesis". This paper provides a short analysis of the concept along with a description of used approaches to characterize hormetic relationships. The whole hormetic curve can be divided into three zones: I - a lag-zone where no changes are observed with increasing dose; II - a zone where beneficial/adverse effects are observed, and III - a zone where the effects are opposite to those seen in zone II. Some approaches are proposed to analyze the molecular components involved in the development of the hormetic character of dose-response relationships with the use of specific genetic lines or inhibitors of regulatory pathways. The discussion is then extended to suggest a new parameter (half-width of the hormetic curve at zone II) for quantitative characterization of the hormetic curve. The problems limiting progress in the development of the hormesis concept such as low reproducibility and predictability may be solved, at least partly, by deciphering the molecular mechanisms underlying the hormetic dose-effect relationship.

  5. Development of a bioassay to screen for chemicals mimicking the anti-aging effects of calorie restriction

    Energy Technology Data Exchange (ETDEWEB)

    Chiba, Takuya, E-mail: takuya@nagasaki-u.ac.jp [Department of Investigative Pathology, Graduate School of Biomedical Sciences, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523 (Japan); Tsuchiya, Tomoshi [Division of Surgical Oncology, Graduate School of Biomedical Sciences, Nagasaki University, 1-7-1 Sakamoto, Nagasaki 852-8501 (Japan); Komatsu, Toshimitsu; Mori, Ryoichi; Hayashi, Hiroko [Department of Investigative Pathology, Graduate School of Biomedical Sciences, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523 (Japan); Shimano, Hitoshi [Department of Internal Medicine (Endocrinology and Metabolism), Graduate School of Comprehensive Human Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba Ibaraki 305-8575 (Japan); Spindler, Stephen R. [Department of Biochemistry, Room 5478, Boyce Hall, University of California - Riverside, Riverside, CA 92521 (United States); Shimokawa, Isao [Department of Investigative Pathology, Graduate School of Biomedical Sciences, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523 (Japan)

    2010-10-15

    Research highlights: {yields} We identified four sequence motifs lying upstream of putative pro-longevity genes. {yields} One of these motifs binds to HNF-4{alpha}. {yields} HNF-4{alpha}/PGC-1{alpha} could up-regulate the transcription of a reporter gene linked to this motif. {yields} The reporter system described here could be used to screen candidate anti-aging molecules. -- Abstract: Suppression of the growth hormone/insulin-like growth factor-I pathway in Ames dwarf (DF) mice, and caloric restriction (CR) in normal mice extends lifespan and delays the onset of age-related disorders. In combination, these interventions have an additive effect on lifespan in Ames DF mice. Therefore, common signaling pathways regulated by DF and CR could have additive effects on longevity. In this study, we tried to identity the signaling mechanism and develop a system to assess pro-longevity status in cells and mice. We previously identified genes up-regulated in the liver of DF and CR mice by DNA microarray analysis. Motif analysis of the upstream sequences of those genes revealed four major consensus sequence motifs, which have been named dwarfism and calorie restriction-responsive elements (DFCR-REs). One of the synthesized sequences bound to hepatocyte nuclear factor-4{alpha} (HNF-4{alpha}), an important transcription factor involved in liver metabolism. Furthermore, using this sequence information, we developed a highly sensitive bioassay to identify chemicals mimicking the anti-aging effects of CR. When the reporter construct, containing an element upstream of a secreted alkaline phosphatase (SEAP) gene, was co-transfected with HNF-4{alpha} and its regulator peroxisome proliferator-activated receptor (PPAR) {gamma} coactivator-1{alpha} (PGC-1{alpha}), SEAP activity was increased compared with untransfected controls. Moreover, transient transgenic mice established using this construct showed increased SEAP activity in CR mice compared with ad libitum-fed mice. These data

  6. Hormetic effect of panaxatriol saponins confers neuroprotection in PC12 cells and zebrafish through PI3K/AKT/mTOR and AMPK/SIRT1/FOXO3 pathways

    Science.gov (United States)

    Zhang, Chao; Li, Chuwen; Chen, Shenghui; Li, Zhiping; Ma, Lijuan; Jia, Xuejing; Wang, Kai; Bao, Jiaolin; Liang, Yeer; Chen, Meiwan; Li, Peng; Su, Huanxing; Lee, Simon Ming Yuen; Liu, Kechun; Wan, Jian-Bo; He, Chengwei

    2017-01-01

    Hormesis is an adaptive response of living organisms to a moderate stress. However, its biomedical implication and molecular mechanisms remain to be intensively investigated. Panaxatriol saponins (PTS) is the major bioactive components extracted from Panax notoginseng, a widely used herbal medicine for cerebrovascular diseases. This study aims to examine the hormetic and neuroprotective effects of PTS in PC12 cells and zebrafish Parkinson’s disease (PD) models. Our results demonstrated that PTS stimulated PC12 cell growth by about 30% at low doses, while PTS at high doses inhibited cell growth, which is a typical hormetic effect. Moreover, we found that low dose PTS pretreatment significantly attenuated 6-OHDA-induced cytotoxicity and up-regulated PI3K/AKT/mTOR cell proliferation pathway and AMPK/SIRT1/FOXO3 cell survival pathway in PC12 cells. These results strongly suggested that neuroprotective effects of PTS may be attributable to the hormetic effect induced by PTS through activating adaptive response-related signaling pathways. Notably, low dose PTS could significantly prevent the 6-OHDA-induced dopaminergic neuron loss and improve the behavior movement deficiency in zebrafish, whereas relative high dose PTS exhibited neural toxicity, further supporting the hormetic and neuroprotective effects of PTS. This study indicates that PTS may have the potential in the development of future therapeutic medicines for PD. PMID:28112228

  7. A Theoretical Model for the Hormetic Dose-response Curve for Anticancer Agents.

    Science.gov (United States)

    Yoshimasu, Tatsuya; Ohashi, Takuya; Oura, Shoji; Kokawa, Yozo; Kawago, Mitsumasa; Hirai, Yoshimitsu; Miyasaka, Miwako; Nishiguchi, Haruka; Kawashima, Sayoko; Yata, Yumi; Honda, Mariko; Fujimoto, Takahiro; Okamura, Yoshitaka

    2015-11-01

    In the present article, we quantitatively evaluated the dose-response relationship of hormetic reactions of anticancer agents in vitro. Serial dilutions of gemcitabine, cisplatin, 5-fluorouracil, vinorelbine, and paclitaxel were administered to the A549 non-small-cell lung cancer cell line. The bi-phasic sigmoidal curve with hormetic and cytotoxic effects is given by the formula y=(a-b/(1+exp(c(*)log(x)-d)))/(1+exp(e(*)log(x)-f)), that was used to perform a non-linear least square regression. The dose-responses of the five anticancer agents were fitted to this equation. Gemcitabine and 5-fluorouracil, which had the lowest ED50 for their hormetic reaction, had the most pronounced promotive effects out of the five anticancer agents tested. The hormetic reaction progressed exponentially with culturing time. Our theoretical model will be useful in predicting how hormetic reactions affect patients with malignant tumors.

  8. Anti-aging effects of Piper cambodianum P. Fourn. extract on normal human dermal fibroblast cells and a wound-healing model in mice

    Directory of Open Access Journals (Sweden)

    Lee H

    2016-07-01

    Full Text Available Hyunji Lee,1 Youngeun Hong,1 So Hee Kwon,2 Jongsun Park,1 Jisoo Park1 1Department of Pharmacology and Medical Science, Metabolic Diseases and Cell Signaling Laboratory, Research Institute for Medical Sciences, College of Medicine, Chungnam National University, Daejeon, 2Department of Pharmacy, College of Pharmacy, Yonsei Institute of Pharmaceutical Sciences, Yonsei University, Incheon, South Korea Background: Aging of skin is associated with environmental factors such as ultraviolet rays, air pollution, gravity, and genetic factors, all of which can lead to wrinkling of skin. Previous reports suggest that the wound repair is impaired by the aging process and strategies to manipulate the age-related wound healing are necessary in order to stimulate repair.Objective: Several traditional plant extracts are well-known for their properties of skin protection and care. Piper cambodianum P. Fourn. (PPF, a member of Piperacecae, is a plant found in Vietnam that might have therapeutic properties. Therefore, the effects of PPF stem and leaf extract on aging process were investigated in vitro and in vivo.Methods: PPF extract dissolved in methanol was investigated using Western blotting, real-time quantitative reverse transcription-polymerase chain reaction, flow cytometry, and cell wound-healing assays. We assessed the anti-aging effect of PPF in mouse using the wound-healing assay. The results were analyzed by Student’s unpaired t-test; *P<0.05 and **P<0.01 were considered to indicate significant and highly significant values, respectively, compared with corresponding controls.Results: PPF treatment demonstrated in vitro and in vivo anti-aging activity. Western blot analysis of PPF-treated normal human dermal fibroblast cells showed a dose-dependent increase in the expression of extracellular matrix genes such as collagen and elastin, but decreased expression of the aging gene matrix metalloproteinase-3. Quantitative polymerase chain reaction showed

  9. The clinical effect and analysis of ginseng anti-aging facial mask%人参抗衰老面膜临床功效测试与分析

    Institute of Scientific and Technical Information of China (English)

    朱丽平; 孙常磊; 李子安

    2016-01-01

    ObjectiveTo investigate the clinical effect of ginseng anti-aging facial mask,we evaluated the effect of this product on anti-aging.MethodsIn the short-acting test,we test skin stratum corneum hydration. In the long-acting test,we test skin stratum corneum hydration, transepidermal water loss(TWEL values),elasticity R2 values and use VISIA Intelligent camera system to get pictures of left,front and right face.ResultsAnalysis showed that ginseng anti-aging facial mask could improve skin stratum corneum hydration in the short-acting test,as well as,ginseng anti-aging facial mask could improve skin stratum corneum hydration and elasticity values,reduce transepidermal water loss and facial wrinkle in the long-acting test.Conclusion In a word,ginseng facial mask has excellent anti-aging effect.%目的:对人参抗衰老面膜进行临床功效测试,包括短效测试和长效测试,评价了面膜抗衰老的功效。方法:短效测试中,测试皮肤含水量(皮肤角质层含水量)。在长效测试中,测试皮肤含水量(皮肤角质层含水量)、皮肤经皮水分流失值(TEWL值)、皮肤弹性R2值,并用VISIA智能拍照系统拍摄左侧脸、正脸、右侧脸。结果:分析结果显示,在短效测试中,使用人参抗衰老面膜,能够显著提高皮肤含水量;在长效测试中,使用人参抗衰老面膜,能够显著提高皮肤含水量,提高皮肤弹性,降低TEWL值,减少面部皱纹。结论:人参抗衰老面膜具有良好的抗衰老功效。

  10. What is an anti-aging treatment?

    Science.gov (United States)

    Gems, David

    2014-10-01

    Key objectives of biogerontology are to understand the biology of aging and to translate scientific insight into interventions that improve late-life health - or anti-aging treatments. In this context, when considering the problem of how to effect translational research, it is useful to have a clear, consensus view on what exactly constitutes an anti-aging treatment. This essay critically assesses the understanding of this concept common among biogerontologists, and proposes a new definition. A current conception of anti-aging treatment imagines a primary cause of aging that is causally upstream of, and the cause of, all age-related pathology. Intervening in this aging process thus protects against the totality of age-related diseases. However, this underlying aging process remains an abstraction. By contrast, what is demonstrable is that interventions in model organisms can improve late-life health and extend lifespan. Furthermore, a safe deduction is that treatments that extend lifespan do so by reducing age-related pathology, both florid and subtle. What is currently identifiable about aging (i.e. senescence) is that it is a very complex disease syndrome, likely involving a number of biological mechanisms. Treatments that substantially extend lifespan must suppress multiple pathologies that otherwise limit lifespan, but whether they suppress the entire aging process remains undemonstrated. A more pragmatic and realistic definition of anti-aging treatment is any preventative approach to reduce late-life pathology, based on the understanding that senescence is a disease syndrome. This definition would encompass preventative approaches aimed at both broad and narrow spectra of age-related pathologies. Its adoption would facilitate translation, since it would shift the emphasis to medical practice, particularly the introduction of preventative approaches. Narrow spectrum anti-aging treatments (e.g. the cardiovascular polypill) could establish a practice that

  11. Anti-aging effect of adipose-derived stem cells in a mouse model of skin aging induced by D-galactose.

    Directory of Open Access Journals (Sweden)

    Shengchang Zhang

    Full Text Available INTRODUCTION: Glycation products accumulate during aging of slowly renewing tissue, including skin, and are suggested as an important mechanism underlying the skin aging process. Adipose-derived cells are widely used in the clinic to treat ischemic diseases and enhance wound healing. Interestingly, adipose-derived stem cells (ASCs are also effective in anti-aging therapy, although the mechanism underlying their effects remains unknown. The purpose of the present study was to examine the anti-aging effect of ASCs in a D-galactose-induced aging animal model and to clarify the underlying mechanism. MATERIALS AND METHODS: Six-week-old nude mice were subcutaneously injected with D-gal daily for 8 weeks. Two weeks after completion of treatment, mice were randomized to receive subcutaneous injections of 106 green fluorescent protein (GFP-expressing ASCs, aminoguanidine (AG or phosphate-buffered saline (PBS. Control mice received no treatment. We examined tissue histology and determined the activity of senescence-associated molecular markers such as superoxide dismutase (SOD and malondialdehyde (MDA. RESULTS: Transplanted ASCs were detectable for 14 days and their GFP signal disappeared at day 28 after injection. ASCs inhibited advanced glycation end product (AGE levels in our animal model as well as increased the SOD level and decreased the MDA level, all of which act to reverse the aging phenotype in a similar way to AG, an inhibitor of AGE formation. Furthermore, ASCs released angiogenic factors in vivo such as vascular endothelial growth factor, suggesting a skin trophic effect. CONCLUSIONS: These results demonstrate that ASCs may contribute to the regeneration of skin during aging. In addition, the data shows that ASCs provide a functional benefit by glycation suppression, antioxidation, and trophic effects in a mouse model of aging.

  12. Skin delivery of epigallocatechin-3-gallate (EGCG) and hyaluronic acid loaded nano-transfersomes for antioxidant and anti-aging effects in UV radiation induced skin damage.

    Science.gov (United States)

    Avadhani, Kiran S; Manikkath, Jyothsna; Tiwari, Mradul; Chandrasekhar, Misra; Godavarthi, Ashok; Vidya, Shimoga M; Hariharapura, Raghu C; Kalthur, Guruprasad; Udupa, Nayanabhirama; Mutalik, Srinivas

    2017-11-01

    The present work attempts to develop and statistically optimize transfersomes containing EGCG and hyaluronic acid to synergize the UV radiation-protective ability of both compounds, along with imparting antioxidant and anti-aging effects. Transfersomes were prepared by thin film hydration technique, using soy phosphatidylcholine and sodium cholate, combined with high-pressure homogenization. They were characterized with respect to size, polydispersity index, zeta potential, morphology, entrapment efficiency, Fourier Transform Infrared Spectroscopy (FTIR), Differential Scanning Calorimetry (DSC), X-ray Diffraction (XRD), in vitro antioxidant activity and ex vivo skin permeation studies. Cell viability, lipid peroxidation, intracellular ROS levels and expression of MMPs (2 and 9) were determined in human keratinocyte cell lines (HaCaT). The composition of the transfersomes was statistically optimized by Design of Experiments using Box-Behnken design with four factors at three levels. The optimized transfersome formulation showed vesicle size, polydispersity index and zeta potential of 101.2 ± 6.0 nm, 0.245 ± 0.069 and -44.8 ± 5.24 mV, respectively. FTIR and DSC showed no interaction between EGCG and the selected excipients. XRD results revealed no form conversion of EGCG in its transfersomal form. The optimized transfersomes were found to increase the cell viability and reduce the lipid peroxidation, intracellular ROS and expression of MMPs in HaCaT cells. The optimized transfersomal formulation of EGCG and HA exhibited considerably higher skin permeation and deposition of EGCG than that observed with plain EGCG. The results underline the potential application of the developed transfersomes in sunscreen cream/lotions for improvement of UV radiation-protection along with deriving antioxidant and anti-aging effects.

  13. Anti-aging pharmacology: Promises and pitfalls.

    Science.gov (United States)

    Vaiserman, Alexander M; Lushchak, Oleh V; Koliada, Alexander K

    2016-11-01

    Life expectancy has grown dramatically in modern times. This increase, however, is not accompanied by the same increase in healthspan. Efforts to extend healthspan through pharmacological agents targeting aging-related pathological changes are now in the spotlight of geroscience, the main idea of which is that delaying of aging is far more effective than preventing the particular chronic disorders. Currently, anti-aging pharmacology is a rapidly developing discipline. It is a preventive field of health care, as opposed to conventional medicine which focuses on treating symptoms rather than root causes of illness. A number of pharmacological agents targeting basic aging pathways (i.e., calorie restriction mimetics, autophagy inducers, senolytics etc.) are now under investigation. This review summarizes the literature related to advances, perspectives and challenges in the field of anti-aging pharmacology.

  14. Paradigmenwechsel in der Anti-Aging-Medizin: Hormesis, Target-of-Rapamycin-Komplex und erste Anti-Aging-Pillen // Paradigm Shift in Anti-Aging Medicine: Hormesis, Target of Rapamycin Complex and First Human Anti-Aging-Pills

    Directory of Open Access Journals (Sweden)

    Römmler A

    2016-01-01

    Full Text Available Studies in model organisms have shown that some drugs and lifestyle practices (calorie-restricted diets, regular exercise, e.g. can extend life and health span and protect against the onset of age-related chronic diseases by targeting physiological pathways.brA common mode of action was found via mTOR (mechanistic Target Of Rapamycin pathway signalling. This intracellular protein kinase complex plays a key role in stimulating anabolic and cell growth promoting processes, while inhibiting autophagy. On the other hand, downregulation results in antiproliferative, anticancer and intensive cell-repairing effects leading to life and health span extension and stress resistance. The mTOR complex regulates such basic cell activities and integrates signals from nutrition sensing, energy metabolism, insulin and growth factors, stress and hypoxia.brImportantly, mTOR can be inhibited by some molecules and their analogs (rapamycin, resveratrol, metformin, e.g., which are released naturally from plants, yeast or bacteria to protect against natural enemies. Its dosage resembles an adaptive hormetic response relationship, as high concentrations are toxic and mild doses are associated with anticancer and antiaging effects. This opens up new avenues for their use as „anti-aging pills“ in humans.brRecent human data suggest that metformin, rapamycin and other mTOR-inhibitors could delay heart disease, cancer, cognitive decline and improve survival time in people with diabetes mellitus. In addition, response to influenca vaccine was enhanced by rapamycin in adults with immunosenescence, indicating beneficial anti-aging effects in the elderly.br“Treat aging” is an actual call to recognize aging as an indication appropriate for clinical trials and treatments, as it was recently approved by the Federal Drug Administration (FDA USA. p bKurzfassung/b: Die ansteigende Morbidität und Invalidität in alternden Industrienationen stößt an die Grenzen der Ressourcen

  15. Restudy on the anti-aging effects of the Tibetan kefir yoghourt%再探西藏灵菇发酵奶的抗衰老作用

    Institute of Scientific and Technical Information of China (English)

    薄芯; 李京霞; 叶磊

    2012-01-01

    This study with Tibetan kefir yoghourt for research object, fresh milk and yoghourt as contrasts,to mensurate glutathione peroxidase (GSH-PX),superoxide dimutase(SOD),total cholesterol(TC)、triglyceride(TG)、high-density lipoprotein cholesterol(HDL-C)in serum of mice,amphimixis delitescence and amphimixis duration of fruit fly(Drosophila melanogaster), to explore anti-aging effect of Tibetan kefir yoghourt. The results show that Tibetan kefir yoghourt promote GSH-PX, SOD and HDL-C, and repress TC and TG. Tibetan kefir yoghourt can shorter amphimixis delitescenc and longer amphimixis duration of fruit fly. And there were dose-effect relation of Tibetan kefir yoghourt. All results attest that there is anti-aging effect by Tibetan kefir yoghourt indeed.%以西藏灵菇发酵奶为研究对象,鲜牛奶和普通酸奶为对照,通过测定小鼠血清中的谷胱甘肽过氧化物酶、超氧化物歧化酶,血清总胆固醇、总甘油三酯、高密度脂蛋白胆固醇和果蝇繁殖时的交配潜伏期和交配持续期等指标,探索其抗衰老作用.结果表明:西藏灵菇发酵奶可以提升小鼠血清中谷胱甘肽过氧化物酶和超氧化物歧化酶活性,且都与浓度呈正量效关系;能降低血清总胆固醇和总甘油三酯,提高高密度脂蛋白胆固醇的浓度;使果蝇生殖力能力提高,交配潜伏期缩短,交配持续期延长,且均表现有量效关系.所有数据均证明西藏灵菇发酵奶确实具有一定的抗衰老能力.

  16. Anti-ageing effects of a new Dimethylaminoethanol-based formulation on D-Galactose induced skin ageing model of rat

    Institute of Scientific and Technical Information of China (English)

    YAN Bing-jian; YUAN Feng; ZHAO Cai-ling; LIU Su

    2015-01-01

    Background Dimethylaminoethanol has been widely used to fight against wrinkles, in the field of aesthetic medicine there is an increasing demand for safe and effective Dimethylaminoethanol-based products to counteract the ageing process. Objective To evaluate the anti-ageing effects of a new DMAE-based formulation. Methods 30 male rats were randomly allocated into treatment,D-gal ageing modeland control groups, each of which contained ten rats. Treatment group and D-gal ageing model group were subcutaneously injected with D- galactose prepared in normal saline 125mg·kg-1·d-1 for 42d. Control groups were injected with normal saline for 42 d with same method and dose. From the 18th day,after shaving their hair,the treatment grouprats were injected thisnew DMAE-based formulation at a dose of 1ml per week for 4 weeks in the Dermis of two sides hip skin mark zone.Meanwhile,D-gal ageing model group rats were administrated the same volume of normal saline with same method. Skin specimens were obtained 3days after the last treatment. Dermal collagen density and dermal thickness were evaluated by H&E and Masson-trichrome staining. And mRNA expressions of TGFβ1, Smad3, Type I,Type III Pro-collagen,TIMP-1, MMP-1,were assessed by Real-time quantitative polymerase chain reaction. Results Dermal thickness, dermal collagen density and hydroxyproline content in treatment group increased significantly comparing with D-gal ageing model group. No differences were found in mRNA expression of MMP-1 and Type III Pro-collagen between the treatment group and D-gal ageing model group. In addition, mRNA expression of TGFβ1, Type I Pre-collagen , TIMP1 and smad3 in treatment group were significantly up-regulated in contrast with D-gal ageing model and control group. Conclusion This new DMAE-based formulationcould generate anti-ageing effects by activating collagen synthesisthrough TGF-β1/Smads signaling pathway.

  17. Anti-Aging Effects of the Hanwoo Leg Bone, Foot and Tail Infusions (HLI, HFI and HTI) on Skin Fibroblast

    OpenAIRE

    Seol, Ja young; Yoon, Ji Young; Jeong, Hee Sun; Joo, Nami; Choi, Soon Young

    2016-01-01

    Many researchers revealed that collagen contribute to maintaining the skin’s elasticity and inhibit wrinkling of skin. Korean native cattle (Hanwoo) bone (leg bone, foot and tail) infusion contains the various inorganic materials, collagen and chondroitin sulfate. All of this, a large quantity of collagen is included in Hanwoo infusion. Therefore, this study emphasized on the effects of collagen in the Hanwoo bone infusion. For the first time, Hanwoo bone infusions were directly added to the ...

  18. Anti-amnestic and anti-aging effects of ginsenoside Rg1 and Rb1 and its mechanism of action

    Institute of Scientific and Technical Information of China (English)

    Yong CHENG; Li-hong SHEN; Jun-tian ZHANG

    2005-01-01

    In the present paper, we overview the discovery of new biological activities induced by ginsenoside Rg1 and Rb1 and discuss possible mechanisms of action. Both compounds could increase neural plasticity in efficacy and structure; espe cially Rg1, as one small molecular drug, can increase proliferation and differentia tion of neural progenitor cells in dentate gyms of hippocampus of normal adult mice and global ischemia model in gerbils. This finding has great value for treat ment of Alzheimer's disease and other neurodegenerative disorders which is characterized by neurons loss. Increase of expression of brain derived neurotrophic factor, Bcl-2 and antioxidant enzyme, enhanced new synapse formation, inhibition of apoptosis and calcium overload are also important neuron protective factors. Rg1 and Rb1 have common effects, but there are some differences in pharmacol ogy and mechanism. These differences may attribute to their different chemical structure. Rg1 is panaxtriol with two sugars, while Rb1 is panaxtriol with four sugars.

  19. Effects of Water Deficit and Increased Nitrogen Application in the Late Growth Stage on Physiological Characters of Anti-aging of Leaves in Different Hybrid Rice Varieties

    Institute of Scientific and Technical Information of China (English)

    2012-01-01

    [Objective] This study aimed to explore the effects of water and fertilizer regulation on the function of leaves in the filling stage. [Method] Six hybrid rice vari- eties including prematurely aged types and non-prematurely aged ones were selected as experimental materials. Limiting water treatment (with soil water potential of about -25 kpa) and increasing nitrogen treatment (additional 10% of granular nitrogen fertiliz- er in addition to limiting water treatment) were applied after heading to analyse the rates of aging and physiological responses of anti-aging in different hybrid rice vari- eties under water deficit and increased nitrogen conditions taking regular water and fertilizer as control. [Result] The results showed that water deficit accelerated the leaf senescence, and prematurely aged types-'Zhongyou838', 'Tianyou998' and 'Long- ping601 '-were more markedly affected by water deficit, of which the rates of chloro- phyll degradation were 6%-8% higher compared to that in another three hybrids. In- creasing nitrogen treatment raised chlorophyll content and slowed down its degrada- tion. Water deficit caused the increase of abscisic acid (ABA) content to obviously varying degrees in leaves of six hybried rice varieties. Responses of ABA content in six hybried rice varieties to increased nitrogen fertilizer were not consistent. Except in 'Zhongyou838', ABA content in the other five hybrids had varying degrees of in- crease. The responses of the activity of antioxidant enzymes in different hybried rice varieties were inconsistent. In response to increased nitrogen fertilizer in combination with water deficit, the activity of each antioxidant enzyme changed differently. Water deficit enhanced the accumulation of malondialdehyde (MDA) in leaves of each hybrid rice. The correlation analysis showed that chlorophyll content was extremely signifi- cantly positively correlated to the resistance of each antioxidant enzyme; SOD activity in leaves also positively

  20. Edward F. Adolph Distinguished Lecture: The remarkable anti-aging effects of aerobic exercise on systemic arteries.

    Science.gov (United States)

    Seals, Douglas R

    2014-09-01

    Cardiovascular diseases (CVD) remain the leading cause of morbidity and mortality in modern societies, and advancing age is the major risk factor for CVD. Arterial dysfunction, characterized by large elastic artery stiffening and endothelial dysfunction, is the key event leading to age-associated CVD. Our work shows that regular aerobic exercise inhibits large elastic artery stiffening with aging (optimizes arterial compliance) and preserves endothelial function. Importantly, among previously sedentary late middle-aged and older adults, aerobic exercise improves arterial stiffness and enhances endothelial function in most groups and, therefore, also can be considered a treatment for age-associated arterial dysfunction. The mechanisms by which regular aerobic exercise destiffens large elastic arteries are incompletely understood, but existing evidence suggests that reductions in oxidative stress associated with decreases in both adventitial collagen (fibrosis) and advanced glycation end-products (structural protein cross-linking molecules), play a key role. Aerobic exercise preserves endothelial function with aging by maintaining nitric oxide bioavailability via suppression of excessive superoxide-associated oxidative stress, and by inhibiting the development of chronic low-grade vascular inflammation. Recent work from our laboratory supports the novel hypothesis that aerobic exercise may exert these beneficial effects by directly inducing protection to aging arteries against multiple adverse factors to which they are chronically exposed. Regular aerobic exercise should be viewed as a "first line" strategy for prevention and treatment of arterial aging and a vital component of a contemporary public health approach for reducing the projected increase in population CVD burden.

  1. Studying the Anti-aging Effect of Human Growth Hormone on Human Fibroblast Cells via Telomerase Activity

    Directory of Open Access Journals (Sweden)

    Nader Chaparzadeh

    2010-01-01

    Full Text Available Objective: In recent years, studies have focused on the telomerase for cancer treatmentby repressing telomerase in cancerous cells or prevent cell aging by activating it in theaged cells. Thus, in these studies natural and synthetic agents have been used to repressor activate telomerase. In this research, we investigated the effects of human growth hormone(hGH on aging via evaluation of telomerase activity.Materials and Methods: Primary human foreskin fibroblast cells were isolated, culturedand treated with different concentrations of hGH. BrdU and MTT cell proliferation assaysand cells number counting. Cell aging was assayed by the senescence sensitivegalactosidase staining method. Telomerase activity was measured with a telomerasePCR ELISA kit.Data were analyzed with SPSS software (one-way ANOVA and univariateANOVA.Results: Our results indicated that cells treated with a lower concentration (0.1, 1 ng/mlof hGH had more green color cells (aged cells. Furthermore, cell proliferation increasedwith increasing hGH concentrations (10 to 100 ng/ml which was significant in comparisonwith untreated control cells. TRAP assay results indicated that telomerase activityincreased with increasing hGH concentration, but there was no significant difference. Additionally,more rapid cell growth and telomerase activity was noted in the absence of H2O2when compared with the presence of H2O2, which was significantly different.Conclusion: Although increasing cell proliferation along with increasing hGH concentrationwas confirmed by all cell proliferation assays, only the cell counting test was statisticallysignificant. Thus, it is inconclusive that hGH (up to 100 ng/ml has an anti-agingeffect. Also, because there was no significant difference in the telomerase activity results(in spite of increasing progress along with increasing hGH concentration we can not certainlyconclude that hGH (up to 100 ng/ml impacts telomerase activity.

  2. Anti-Ageing Effects of Sonchus oleraceus L. (pūhā Leaf Extracts on H2O2-Induced Cell Senescence

    Directory of Open Access Journals (Sweden)

    Zong-Quan Ou

    2015-03-01

    Full Text Available Antioxidants protect against damage from free radicals and are believed to slow the ageing process. Previously, we have reported the high antioxidant activity of 70% methanolic Sonchus oleraceus L. (Asteraceae leaf extracts. We hypothesize that S. oleraceus extracts protect cells against H2O2-induced senescence by mediating oxidative stress. Premature senescence of young WI-38 cells was induced by application of H2O2. Cells were treated with S. oleraceus extracts before or after H2O2 stress. The senescence- associated β-galactosidase (SA-β-gal activity was used to indicate cell senescence. S. oleraceus extracts showed higher cellular antioxidant activity than chlorogenic acid in WI-38 cells. S. oleraceus extracts suppressed H2O2 stress-induced premature senescence in a concentration-dependent manner. At 5 and 20 mg/mL, S. oleraceus extracts showed better or equivalent effects of reducing stress-induced premature senescence than the corresponding ascorbic acid treatments. These findings indicate the potential of S. oleraceus extracts to be formulated as an anti-ageing agent.

  3. Anti-ageing effects of Sonchus oleraceus L. (pūhā) leaf extracts on H₂O₂-induced cell senescence.

    Science.gov (United States)

    Ou, Zong-Quan; Rades, Thomas; McDowell, Arlene

    2015-03-12

    Antioxidants protect against damage from free radicals and are believed to slow the ageing process. Previously, we have reported the high antioxidant activity of 70% methanolic Sonchus oleraceus L. (Asteraceae) leaf extracts. We hypothesize that S. oleraceus extracts protect cells against H2O2-induced senescence by mediating oxidative stress. Premature senescence of young WI-38 cells was induced by application of H2O2. Cells were treated with S. oleraceus extracts before or after H2O2 stress. The senescence- associated β-galactosidase (SA-β-gal) activity was used to indicate cell senescence. S. oleraceus extracts showed higher cellular antioxidant activity than chlorogenic acid in WI-38 cells. S. oleraceus extracts suppressed H2O2 stress-induced premature senescence in a concentration-dependent manner. At 5 and 20 mg/mL, S. oleraceus extracts showed better or equivalent effects of reducing stress-induced premature senescence than the corresponding ascorbic acid treatments. These findings indicate the potential of S. oleraceus extracts to be formulated as an anti-ageing agent.

  4. 红菇提取液抗衰老作用初探%Preliminary Exploration about the Anti-aging Effect of Liquid Extraction from the Russula Fruitbodyon

    Institute of Scientific and Technical Information of China (English)

    甘耀坤; 刘英姿; 梁钧淞; 曾诗媛; 余锦荣; 周志云

    2011-01-01

    Objective: To study the influence of Liquid Extraction from the Russula Fruitbodyon on the life- span of Drosophila melanogaster, and discuss preliminarily the anti-ageing effect of Liquid Extraction from the Russula Fruitbodyon. Methods: With three recessive types of drosophila melanogasters as the research object, through drosophila melanogaster' survival tests to obtain the statistics of drosophila melanogaster with average life-span, half-time death, the longest life-span, comparing the effect of four different concentrations influence of Liquid Extraction from the Russula Fruitbodyon: lmg/ml, 5mg/ml, 10mg/ml and 15mg/ml on the Life-span of Drosophila melanogaster. Results: With regard to the average life-span group, the life span of male drosophila rnelanogaster and female drosophila melanogaster delay respectively by 6.94 % and 11.14 % (P both 〈0.01 ) when it is lmg/rnl; the life span of female drosophila melanogaster delay by 7.14 % ( P 〈0.01 ) when it is 5 mg/ ml. With regard to half-time death group, when it is lmg/ml, the life span of male drosophila melanogaster and female drosophila melanogaster delay respectively by 7.51% and 8.45%(P both〈0.01); when it is 5 mg/ml, the life span of female drosophila melanogaster delay by 7.38% ( P 〈0.01 ) . As for the longest life-span group, when they are 5mg/ml and 10mg/ml, the life span of female drosophila melanogaster delay respectively by13.47% and 16.37% ( P both 〈0.01 ) . Conclusion: Liquid extraction from the 1Kussula Fruitbodyon on the drosophila melanogaster's life has certain anti-ageing effect, 1mg/ml of Liquid Extraction from the Russula Fruitbodyon has the best effect.%目的:研究红菇提取液对果蝇寿命的影响,初步探讨红菇提取液的抗衰老作用.方法:以3隐性果蝇为对象,通过果蝇的生存试验主要统计其平均寿命、半数死亡时间和最高寿命,比较1mg/ml、5mg/ml、10mg/ml和15mg/ml四种不同浓度红菇提

  5. 葡萄柚果汁延缓果蝇衰老作用的研究%Effects of Grapefruit Juice on anti-aging for Drosophila melanogaster

    Institute of Scientific and Technical Information of China (English)

    王琦; 郑亚凤; 高慧颖; 余亚白

    2013-01-01

    Using fruit fly (Drosophila melanogaster) survival experiment ,we investigated the antioxidant and anti-aging functions of grapefruit juice in vitro .Low dose (5% )、middle dose (10% ) and high dose (20% ) of grapefruit juice were added into the basic medium for fruit fly respectively ,the average lifespan and the highest lifespan of the fruit flies fed in three groups were calculated .Moreover ,SOD activity and MDA content in the homogenate of fruit fly body were determined . The results showed that , with low , middle and high dosages , the grapefruit juice significantly prolonged the average lifespan of male fruit flies by 8.24% ,20.06% and 13.50% ,respectively ,while 12.04% ,24.93% and 19.33% for it of the female fruit flies , respectively . Feeding grapefruit juice in certain concentrate could improve SOD activity and reduce MDA content for both male and female fruit flies .The data from middle dose group showed the best effects which coincide with the results of fruit fly survival experiment .%通过果蝇生存试验研究葡萄柚果汁的体内抗氧化功效和延缓衰老作用。在果蝇培养基中分别加入5%、10%、20%的葡萄柚果汁,测定果蝇平均寿命和最高寿命;并测定果蝇组织匀浆中的超氧化物歧化酶(SOD )值和丙二醛(MDA )含量。结果显示葡萄柚果汁低、中、高剂量组对雄果蝇平均寿命的延长率分别为8.24%、20.06%和13.50%,对雌果蝇平均寿命的延长经分别为12.04%、24.93%和19.33%,差异均达极显著水平。不同浓度的葡萄柚果汁均能提高雄、雌果蝇体内的SOD活性,降低MDA含量水平。其中添加10%果汁效果最好,与果蝇生存试验结果一致。

  6. Combined anti-inflammatory and anti-AGE drug treatments have a protective effect on intervertebral discs in mice with diabetes.

    Directory of Open Access Journals (Sweden)

    Svenja Illien-Junger

    Full Text Available OBJECTIVE: Diabetes and low back pain are debilitating diseases and modern epidemics. Diabetes and obesity are also highly correlated with intervertebral disc (IVD degeneration and back pain. Advanced-glycation-end-products (AGEs increase reactive-oxygen-species (ROS and inflammation, and are one cause for early development of diabetes mellitus. We hypothesize that diabetes results in accumulation of AGEs in spines and associated spinal pathology via increased catabolism. We present a mouse model showing that: 1 diabetes induces pathological changes to structure and composition of IVDs and vertebrae; 2 diabetes is associated with accumulation of AGEs, TNFα, and increased catabolism spinal structures; and 3 oral-treatments with a combination of anti-inflammatory and anti-AGE drugs mitigate these diabetes-induced degenerative changes to the spine. METHODS: Three age-matched groups of ROP-Os mice were compared: non-diabetic, diabetic (streptozotocin (STZ-induced, or diabetic mice treated with pentosan-polysulfate (anti-inflammatory and pyridoxamine (AGE-inhibitor. Mice were euthanized and vertebra-IVD segments were analyzed by μCT, histology and Immunohistochemistry. RESULTS: Diabetic mice exhibited several pathological changes including loss in IVD height, decreased vertebral bone mass, decreased glycosaminoglycan content and morphologically altered IVDs with focal deposition of tissues highly expressing TNFα, MMP-13 and ADAMTS-5. Accumulation of larger amounts of methylglyoxal suggested that AGE accumulation was associated with these diabetic degenerative changes. However, treatment prevented or reduced these pathological effects on vertebrae and IVD. CONCLUSION: This is the first study to demonstrate specific degenerative changes to nucleus pulposus (NP morphology and their association with AGE accumulation in a diabetic mouse model. Furthermore, this is the first study to demonstrate that oral-treatments can inhibit AGE-induced ROS and

  7. Study on anti-aging effect of Lycium ruthenicum Murr.%黑果枸杞抗衰老作用研究

    Institute of Scientific and Technical Information of China (English)

    田磊; 蒋宝平; 樊晓峰

    2015-01-01

    Objective To study the effect of Lycium ruthenicum Murr. on the learning,memory and antioxi-dant ability of aging mice induced by D-galactose. Methods Aging model was induced by subcutaneously injecting mice with D-galactose. At 6 weeks after administration of aqueous extract of Lycium ruthenicum Murr.,the water maze was used to determine the function of learning and memory,the analytic kit was used to measure the activity of super-oxide dismutase ( SOD),the contents of malondialdehyde ( MDA) and lipid peroxidation ( LPO) in brain of mice,as well as the activity of SOD,the contents of MDA and glutathione peroxidase ( GSH-Px) in serum of mice. Results Lycium ruthenicum Murr. could reduce the escape latency,swimming total path,shorten the time in the near station,sig-nificantly shorten the time and swimming path in the distant station area to stay,increase the residence swimming path in the area close to the table and the average swimming speed of aging model mice in a water maze. Besides,it could reduce MDA content and increase GSH-Px content and SOD activity in serum,increase SOD activity and reduce LPO and MDA contents in brain (P<0. 05,P<0. 01). Conclusion Lycium ruthenicum Murr. has anti-aging effect on ag-ing model mice,and the mechanism may be related to improving learning and memory and enhancing antioxidant ability in aging mice.%目的 观察黑果枸杞对D-半乳糖诱导的小鼠衰老模型的学习记忆力及抗氧化能力的影响. 方法 注射5% D-半乳糖制备小鼠衰老模型,灌胃给予黑果枸杞6周后,水迷宫法测定模型小鼠的空间学习记忆能力,试剂盒法测定脑中丙二醛(MDA)、过氧化脂质(LPO)含量及超氧化物歧化酶(SOD)活力,以及血清中谷胱甘肽过氧化物酶( GSH-Px)、MDA含量及SOD活力. 结果 黑果枸杞能缩短衰老模型小鼠在水迷宫的逃避潜伏期、游泳总路程、近台区停留时间、远台区停留时间、远台区游泳路程,增加衰老小鼠平均游泳速度

  8. Hormesis-based anti-aging products: a case study of a novel cosmetic.

    Science.gov (United States)

    Rattan, Suresh I S; Kryzch, Valérie; Schnebert, Sylvianne; Perrier, Eric; Nizard, Carine

    2013-01-01

    Application of hormesis in aging research and interventions is becoming increasingly attractive and successful. The reason for this is the realization that mild stress-induced activation of one or more stress response (SR) pathways, and its consequent stimulation of repair mechanisms, is effective in reducing the age-related accumulation of molecular damage. For example, repeated heat stress-induced synthesis of heat shock proteins has been shown to have a variety of anti-aging effects on growth and other cellular and biochemical characteristics of normal human skin fibroblasts, keratinocytes and endothelial cells undergoing aging in vitro. Therefore, searching for potential hormetins - conditions and compounds eliciting SR-mediated hormesis - is drawing attention of not only the researchers but also the industry involved in developing healthcare products, including nutriceuticals, functional foods and cosmeceuticals. Here we present the example of a skin care cosmetic as one of the first successful product developments incorporating the ideas of hormesis. This was based on the studies to analyse the molecular effects of active ingredients extracted from the roots of the Chinese herb Sanchi (Panax notoginseng) on gene expression at the level of mRNAs and proteins in human skin cells. The results showed that the ginsenosides extracted from Sanchi induced the transcription of stress genes and increased the synthesis of stress proteins, especially the heat shock protein HSP1A1 or Hsp70, in normal human keratinocytes and dermal fibroblasts. Furthermore, this extract also has significant positive effects against facial wrinkles and other symptoms of facial skin aging as tested clinically, which may be due to its hormetic mode of action by stress-induced synthesis of chaperones involved in protein repair and removal of abnormal proteins. Acceptance of such a hormesis-based product by the wider public could be instrumental in the social recognition of the concept of

  9. CCN family member 2/connective tissue growth factor (CCN2/CTGF has anti-aging effects that protect articular cartilage from age-related degenerative changes.

    Directory of Open Access Journals (Sweden)

    Shinsuke Itoh

    Full Text Available To examine the role of connective tissue growth factor CCN2/CTGF (CCN2 in the maintenance of the articular cartilaginous phenotype, we analyzed knee joints from aging transgenic mice (TG overexpressing CCN2 driven by the Col2a1 promoter. Knee joints from 3-, 14-, 40-, and 60-day-old and 5-, 12-, 18-, 21-, and 24-month-old littermates were analyzed. Ccn2-LacZ transgene expression in articular cartilage was followed by X-gal staining until 5 months of age. Overexpression of CCN2 protein was confirmed through all ages in TG articular cartilage and in growth plates. Radiographic analysis of knee joints showed a narrowing joint space and other features of osteoarthritis in 50% of WT, but not in any of the TG mice. Transgenic articular cartilage showed enhanced toluidine blue and safranin-O staining as well as chondrocyte proliferation but reduced staining for type X and I collagen and MMP-13 as compared with those parameters for WT cartilage. Staining for aggrecan neoepitope, a marker of aggrecan degradation in WT articular cartilage, increased at 5 and 12 months, but disappeared at 24 months due to loss of cartilage; whereas it was reduced in TG articular cartilage after 12 months. Expression of cartilage genes and MMPs under cyclic tension stress (CTS was measured by using primary cultures of chondrocytes obtained from wild-type (WT rib cartilage and TG or WT epiphyseal cartilage. CTS applied to primary cultures of mock-transfected rib chondrocytes from WT cartilage and WT epiphyseal cartilage induced expression of Col1a1, ColXa1, Mmp-13, and Mmp-9 mRNAs; however, their levels were not affected in CCN2-overexpressing chondrocytes and TG epiphyseal cartilage. In conclusion, cartilage-specific overexpression of CCN2 during the developmental and growth periods reduced age-related changes in articular cartilage. Thus CCN2 may play a role as an anti-aging factor by stabilizing articular cartilage.

  10. Koschei the immortal and anti-aging drugs.

    Science.gov (United States)

    Blagosklonny, M V

    2014-12-04

    In Slavic folklore, Koschei the Immortal was bony, thin and lean. Was his condition caused by severe calorie restriction (CR)? CR deactivates the target of rapamycin pathway and slows down aging. But the life-extending effect of severe CR is limited by starvation. What if Koschei's anti-aging formula included rapamycin? And was rapamycin (or another rapalog) combined with commonly available drugs such as metformin, aspirin, propranolol, angiotensin II receptor blockers and angiotensin-converting enzyme inhibitors.

  11. [Anti-aging medicine: science or marketing ?].

    Science.gov (United States)

    Cogan, E

    2015-09-01

    Anti-aging medicine is self defined as a preventive medicine, combining nutritional recommendations, dietary supplements, prescriptions for hormones and various aesthetic techniques. The essential aim is to reduce the risk of aging, both psychically, physically and aesthetically. Although many scientific studies in animals or in vitro models have demonstrated the deleterious role of oxidative stress and of hormonal, vitamin or trace elements deficiencies, the transposition to humans of these findings is marginal and does not justify the therapeutic proposals advocated by the anti aging medicine. These practices are mostly not based on any scientific basis both in the diagnostic and therapeutic fields. These approaches are particularly costly for gullible patients in search of well being and abused by a carefully organized marketing involving tacit complicity of doctors, laboratories and firms producing hormones and dietary supplements and various substances devoted for aesthetic purposes.

  12. The anti-aging effects of growth hormone in healthy older age groups%生长激素在健康老年人中的抗衰老作用

    Institute of Scientific and Technical Information of China (English)

    康冬梅; 林杨; 史虹莉

    2009-01-01

    人生长激素是由腺垂体分泌的由191个氨基酸残基组成的直链多肽,它可以直接或通过胰岛素样生长因子间接地对生长和代谢发挥作用,既往主要用于儿童生长激素缺乏症的治疗.随着预期寿命的延长,人们越来越重视生活质量的提高,各种抗衰老药物不断出现,根据衰老学说之一的神经内分泌衰竭学说,生长激素近来被用于抗衰老治疗.在成人,生长激素可增加去脂肪体重,增加骨密度,改善生活质量.本文就生长激素的一般理化特性和其在健康老年人群中抗衰老的作用作一综述.%Human growth hormone is secreted by pituitary and is composed of 191 amino acid,which plays a role directly or through insulin-like growth factor indirectly in the growth and metabolism, mainly used in the treatment of child with growth hormone deficiency formerly. With the increase in life expectancy,attention to improve the quality of life is increased, and all kinds of anti-aging drugs emerge. According to neuroendocrine failure theory,growth hormone has been used for anti-aging treatment. In adults the success criteria are metabolic parameters, the increase of non-fatty body mass, increase of bone density and improvement of subjective quality of life. This article will summarize the general physical and chemical properties of growth hormone and its anti-aging effects in healthy older age groups.

  13. Anti-Ageing Cultures, Biopolitics and Globalisation

    Directory of Open Access Journals (Sweden)

    Brett Neilson

    2011-11-01

    Full Text Available In March 2004, the author attended the Inaugural International Conference on Longevity at the Sydney Exhibition and Convention Centre in Darling Harbour. As a cultural researcher interested in the interactions between demographic shifts, capitalist globalisation and changing forms of political power, the prospect of a direct encounter with the debates and practices surrounding the burgeoning field of anti-ageing medicine promised a means to observe the complex cultural dynamics of population ageing at play. This article explores the discord the atuhor witnessed; a quarrel that, despite the march of technological advance, attests the ongoing conflict in the nexus where politics meets life.

  14. 角鲨烯和维生素E抗皮肤衰老作用的比较研究%Comparative study on anti-aging effect of squalene and vitamin E to skin

    Institute of Scientific and Technical Information of China (English)

    雷茜茜; 赵松林; 陈卫军; 宋菲

    2013-01-01

    研究角鲨烯对皮肤的抗衰老作用并与维生素E进行比较.在10%新鲜猪皮匀浆中分别添加体积为20、40、60、80、100μL的角鲨烯和维生素E,测定猪皮组织匀浆中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)活力,并通过吸光值间接反映了蛋白质羰基和蛋白质氢过氧化物含量变化,以空白作为对照.结果表明角鲨烯能显著提高猪皮SOD、GSH-Px和CAT的活力,同时能显著降低皮肤中蛋白质羰基和蛋白质氢过氧化物含量,且效果较维生素E明显.表明角鲨烯对皮肤有一定的抗衰老作用.%Research about anti-aging effect of squalene and vitamin E to skin where they were mixed with 10% fresh pigskin slurry with a volume of 20,40,60,80,100μL of squalene.The activity of superoxide dismutase(SOD),glutathione peroxidase(GSH-Px)and catalase(CAT) of the pig skin slurry were evaluated,the absorption value reacted indirectly the change of protein carbonyl groups and protein hydroperoxides content,and the blank as a control.The results showed that squalene could remarkably improve SOD,GSH-Px and CAT activity of the pig skin.It also significantly reduced protein carbonyl groups and protein hydroperoxides content of the pig skin,and the effect of squalene was better than vitamin E.Taken togrther results indicated that squalene had anti-aging effect on the skin.

  15. Formulation and in Vivo Evaluation for Anti-Aging Effects of an Emulsion Containing Basil Extract Using non- Invasive Biophysical Techniques

    Directory of Open Access Journals (Sweden)

    N. Akhtar

    2011-12-01

    Full Text Available Background and the purpose of study: Skin aging is a complex process induced by constant exposure to ultraviolet (UV irradiation and damages human skin. UV generates reactive oxygen species leading to collagen deficiency and eventually skin wrinkling. Basil contains a number of phenolics and favonoids which possess antioxidant properties. The aim of this study was to formulate and investigate the antiaging potential of a cream containing Basil extract. Methods: A single blinded study was conducted using non-invasive methods. Formulation containing 3% of the concentrated extract of Basil was developed by entrapping in the inner aqueous phase of w/o emulsion and base contained no extract. Both creams were stored at different storage conditions of 8°C, 25°C, 40°C and 40°C+ 75% relative humidity to predict their stabilities. The formulation and base were evaluated for their effects on various skin parameters i.e., moisture and trans epidermal water loss (TEWL, volume, energy and surface evaluation of the living skin (SELS. Results: Significant effects (p≤0.05 were observed for both creams in the case of TEWL. The base showed insignificant (p≤0.05 while formulation showed significant effects on skin moisture. Volume, SELS SEr (skin roughness, SEsc (skin scaliness, SEsm (skin smoothness, SEw (skin wrinkles parameter showed significant decline while texture parameter of ‘Energy' showed significant increase. Conclusion: The results statistically indicated that the active formulation containg extract of Basil exert antiaging effects when applied topically.

  16. Extracts of Tsai Tai (Brassica chinensis): enhanced antioxidant activity and anti-aging effects both in vitro and in Caenorhabditis elegans.

    Science.gov (United States)

    Chen, Jing; Zhang, Ju; Xiang, Yanxia; Xiang, Limin; Liu, Yongmei; He, Xiangjiu; Zhou, Xiaoju; Liu, Xin; Huang, Zebo

    2016-02-01

    Tsai Tai is one of the most widely consumed Brassica vegetables in Asian countries because of its good taste and its nutritional benefits. This study evaluated the antioxidant capacity and possible associated health benefits of 3 Tsai Tai (Brassica chinensis) varieties, namely, Hon Tsai Tai, Pak Choi and Choi Sum. The DPPH radical scavenging ability and reducing power assays were performed to evaluate the in vitro activities of the extracts. Caenorhabditis elegans was used as an in vivo model for evaluation of beneficial health effects, including antioxidant activity and delayed aging. In vitro, the Hon Tsai Tai extract exhibited higher antioxidant activities than Pak Choi and Choi Sum, and the total phenolic contents were significantly correlated with the DPPH and RP values. In vivo, the three assayed Tsai Tai extracts significantly increased resistance against paraquat-induced oxidative stress with an increase in survival rates from 15% to 28% compared with controls. However, only the extract from Hon Tsai Tai significantly prolonged the lifespan of Caenorhabditis elegans, with an 8% increase in the mean lifespan with respect to controls. Further evidence of antioxidant protection was obtained by assessing ROS production via the DCF assay. The analyses of intracellular SOD activity and MDA content confirmed the existence of an antioxidant protective effect. These results suggest that Tsai Tai might serve as a good source of natural antioxidants, and in particular, Hon Tsai Tai could be explored as a potential dietary supplement to retard aging.

  17. Anti-Aging and Tissue Regeneration Ability of Policosanol Along with Lipid-Lowering Effect in Hyperlipidemic Zebrafish via Enhancement of High-Density Lipoprotein Functionality.

    Science.gov (United States)

    Lee, Eun-Young; Yoo, Jeong-Ah; Lim, So-Mang; Cho, Kyung-Hyun

    2016-04-01

    We investigated the tissue regeneration and lipid-lowering effects of policosanol (PCO) by employing a hyperlipidemic zebrafish model. A reconstituted high-density lipoprotein containing policosanol (PCO-rHDL) facilitated greater cell growth and replication with less apoptosis and reactive oxygen species (ROS) production in BV-2 microglial cell lines. From in vivo study, injection of rHDL containing apolipoprotein A-I (ApoA-I) caused 76 ± 4% (p = 0.01) greater tissue regeneration activity than the phosphate-buffered saline (PBS) control, whereas PCO-rHDL caused 94 ± 7% (p = 0.002) increased regeneration. PCO in ethanol (EtOH) showed lower cholesteryl ester transfer protein (CETP) inhibitory ability than did anacetrapib, whereas PCO-rHDL showed higher inhibitory ability than anacetrapib, suggesting a synergistic effect between PCO and rHDL. Following 9 weeks of PCO consumption, the PCO group (0.003% PCO in Tetrabit) showed the highest survivability (80%), whereas normal diet (ND) and high-cholesterol diet (HCD) control groups showed 67% and 70% survival rates, respectively. Supplementation with a HCD resulted in two-fold elevation of CETP activity along with 3- and 2.5-fold increases in serum total cholesterol (TC) and triglycerides (TGs) levels, respectively. Consumption of PCO for 9 weeks resulted in 40 ± 5% (p = 0.01 vs. HCD) and 33 ± 4% (p = 0.02 vs. HCD) reduction of TC and TGs levels, respectively. Serum high-density lipoprotein cholesterol (HDL-C) level increased up to 37 ± 2 mg/dL (p = 0.004), whereas the percentage of HDL-C/TC increased up to 20 ± 2% from 5 ± 1% compared to the HCD control. The serum glucose level was reduced to 47 ± 2% (p = 0.002) compared to the HCD control. Fatty liver change and hepatic inflammation levels were remarkably increased upon HCD consumption and were two-fold higher than that under ND. However, the PCO group showed 58 ± 5% (p = 0.001) and 50 ± 3

  18. Caloric restriction confers persistent anti-oxidative, pro-angiogenic, and anti-inflammatory effects and promotes anti-aging miRNA expression profile in cerebromicrovascular endothelial cells of aged rats.

    Science.gov (United States)

    Csiszar, Anna; Gautam, Tripti; Sosnowska, Danuta; Tarantini, Stefano; Banki, Eszter; Tucsek, Zsuzsanna; Toth, Peter; Losonczy, Gyorgy; Koller, Akos; Reglodi, Dora; Giles, Cory B; Wren, Jonathan D; Sonntag, William E; Ungvari, Zoltan

    2014-08-01

    In rodents, moderate caloric restriction (CR) without malnutrition exerts significant cerebrovascular protective effects, improving cortical microvascular density and endothelium-dependent vasodilation, but the underlying cellular mechanisms remain elusive. To elucidate the persisting effects of CR on cerebromicrovascular endothelial cells (CMVECs), primary CMVECs were isolated from young (3 mo old) and aged (24 mo old) ad libitum-fed and aged CR F344xBN rats. We found an age-related increase in cellular and mitochondrial oxidative stress, which is prevented by CR. Expression and transcriptional activity of Nrf2 are both significantly reduced in aged CMVECs, whereas CR prevents age-related Nrf2 dysfunction. Expression of miR-144 was upregulated in aged CMVECs, and overexpression of miR-144 significantly decreased expression of Nrf2 in cells derived from both young animals and aged CR rats. Overexpression of a miR-144 antagomir in aged CMVECs significantly decreases expression of miR-144 and upregulates Nrf2. We found that CR prevents age-related impairment of angiogenic processes, including cell proliferation, adhesion to collagen, and formation of capillary-like structures and inhibits apoptosis in CMVECs. CR also exerts significant anti-inflammatory effects, preventing age-related increases in the transcriptional activity of NF-κB and age-associated pro-inflammatory shift in the endothelial secretome. Characterization of CR-induced changes in miRNA expression suggests that they likely affect several critical functions in endothelial cell homeostasis. The predicted regulatory effects of CR-related differentially expressed miRNAs in aged CMVECs are consistent with the anti-aging endothelial effects of CR observed in vivo. Collectively, we find that CR confers persisting anti-oxidative, pro-angiogenic, and anti-inflammatory cellular effects, preserving a youthful phenotype in rat cerebromicrovascular endothelial cells, suggesting that through these effects CR may

  19. Anti-aging cosmetics and its efficacy assessment methods

    Science.gov (United States)

    Li, Xiang

    2015-07-01

    The mechanisms of skin aging, the active ingredients used in anti-aging cosmetics and evaluation methods for anti-aging cosmetics were surmised in this paper. And the mechanisms of skin aging were introduced in the intrinsic and extrinsic ways. Meanwhile, the anti-aging cosmetic active ingredients were classified in accordance with the mechanism of action. Various evaluation methods such as human evaluation, in vitro evaluation were also summarized.

  20. Resveratrol and rapamycin: are they anti-aging drugs?

    Science.gov (United States)

    Kaeberlein, Matt

    2010-02-01

    Studies of the basic biology of aging have advanced to the point where anti-aging interventions, identified from experiments in model organisms, are beginning to be tested in people. Resveratrol and rapamycin, two compounds that target conserved longevity pathways and may mimic some aspects of dietary restriction, represent the first such interventions. Both compounds have been reported to slow aging in yeast and invertebrate species, and rapamycin has also recently been found to increase life span in rodents. In addition, both compounds also show impressive effects in rodent models of age-associated diseases. Clinical trials are underway to assess whether resveratrol is useful as an anti-cancer treatment, and rapamycin is already approved for use in human patients. Compounds such as these, identified from longevity studies in model organisms, hold great promise as therapies to target multiple age-related diseases by modulating the molecular causes of aging.

  1. Validation of anti-aging drugs by treating age-related diseases.

    Science.gov (United States)

    Blagosklonny, Mikhail V

    2009-03-28

    Humans die from age-related diseases, which are deadly manifestations of the aging process. In order to extend life span, an anti-aging drug must delay age-related diseases. All together age-related diseases are the best biomarker of aging. Once a drug is used for treatment of any one chronic disease, its effect against other diseases (atherosclerosis, cancer, prostate enlargement, osteoporosis, insulin resistance, Alzheimer's and Parkinson's diseases, age-related macular degeneration) may be evaluated in the same group of patients. If the group is large, then the anti-aging effect could be validated in a couple of years. Startlingly, retrospective analysis of clinical and preclinical data reveals four potential anti-aging modalities.

  2. Formulasi dan Uji Efek Anti-Aging dari Krim yang Mengandung Minyak Alpukat (Avocado oil)

    OpenAIRE

    Arhandhi, Cut Putri

    2015-01-01

    Background: Anti-aging is a preparation that is useful to prevent or slow down the effects of aging such as the wrinkles, black spots, rough skin and enlarged pores. Anti-aging therapy would be better if done as early as possible, when all functions of the body's cells are still healthy and functioning properly. Avocado oil contains vitamin A which applied to prevent dry skin, as well as vitamin E those are very effective in preventing wrinkles, slow the aging process and vitamin C can help b...

  3. Resveratrol and related stilbenes: their anti-aging and anti-angiogenic properties.

    Science.gov (United States)

    Kasiotis, Konstantinos M; Pratsinis, Harris; Kletsas, Dimitris; Haroutounian, Serkos A

    2013-11-01

    Dietary stilbenes comprise a class of natural compounds that display significant biological activities of medicinal interest. Among them, their antioxidant, anti-aging and anti-angiogenesic properties are well established and subjects of numerous research endeavors. This mini-review aspires to account and present the literature reports published on research concerning various natural and synthetic stilbenes, such as trans-resveratrol. Special focus was given to most recent research findings, while the mechanisms underlying their anti-aging and anti-angiogenic effects as well as the respective signaling pathways involved were also presented and discussed.

  4. Repurposing FDA-approved drugs for anti-aging therapies.

    Science.gov (United States)

    Snell, Terry W; Johnston, Rachel K; Srinivasan, Bharath; Zhou, Hongyi; Gao, Mu; Skolnick, Jeffrey

    2016-11-01

    There is great interest in drugs that are capable of modulating multiple aging pathways, thereby delaying the onset and progression of aging. Effective strategies for drug development include the repurposing of existing drugs already approved by the FDA for human therapy. FDA approved drugs have known mechanisms of action and have been thoroughly screened for safety. Although there has been extensive scientific activity in repurposing drugs for disease therapy, there has been little testing of these drugs for their effects on aging. The pool of FDA approved drugs therefore represents a large reservoir of drug candidates with substantial potential for anti-aging therapy. In this paper we employ FINDSITE(comb), a powerful ligand homology modeling program, to identify binding partners for proteins produced by temperature sensing genes that have been implicated in aging. This list of drugs with potential to modulate aging rates was then tested experimentally for lifespan and healthspan extension using a small invertebrate model. Three protein targets of the rotifer Brachionus manjavacas corresponding to products of the transient receptor potential gene 7, ribosomal protein S6 polypeptide 2 gene, or forkhead box C gene, were screened against a compound library consisting of DrugBank drugs including 1347 FDA approved, non-nutraceutical molecules. Twenty nine drugs ranked in the top 1 % for binding to each target were subsequently included in our experimental analysis. Continuous exposure of rotifers to 1 µM naproxen significantly extended rotifer mean lifespan by 14 %. We used three endpoints to estimate rotifer health: swimming speed (mobility proxy), reproduction (overall vitality), and mitochondria activity (cellular senescence proxy). The natural decline in swimming speed with aging was more gradual when rotifers were exposed to three drugs, so that on day 6, mean swimming speed of females was 1.19 mm/s for naproxen (P = 0.038), 1.20 for fludarabine (P = 0

  5. Effect of light power density on the anti-aging property ot light-cure composite resin%光功率密度对光固化复合树脂耐老化性能的影响

    Institute of Scientific and Technical Information of China (English)

    韩冰; 王晓燕; 高学军

    2011-01-01

    目的:比较不同引发光功率密度对光周化复合树脂耐老化性能的影响.方法:选择光功率密度不同的3种引发固化光模式:(1)模式A,低光功率密度500 mW/cm2,22 s;(2)模式B,高光功率密度1100 mW/cm2,10 s;(3)模式C,渐强式光功率密度,0~650 mW/cm2,5 s,1 100 mW/cm2,8.5 s.3种模式总能量密度均为11 J/cm2.用不同光引发模式同化复合树脂样本,随机分为4绀(n=15),其中3组进行老化,分别浸泡于乙醇中24 h、7 d和30 d.对照组为纯水浸泡24 h.使用显微硬度仪测量样本表面维氏(VHN)硬度.同时使用Acuvol聚合收缩仪测定不同光功率密度引发光固化复合树脂的聚合收缩率(n=7).结果:不同引发光功率密度同化复合树脂的表面硬度为模式A 44.26±6.16,模式B 42.31±2.33,模式C 45.60±2.76.乙醇老化24 h后,树脂表面硬度均有明显下降(P0.05).乙醇老化30 d后,样本表面硬度不再继续降低,与7 d组相比差异无统计学意义(P>0.05),表面硬度值为模式A 28.53±0.86,模式B 28.55±1.53,模式C 29.08±1.60.不同光功率密度模式固化复合树脂的聚合收缩率分别为模式A2.67%±0.28%,模式B 2.76%±0.29%,模式C2.73%±0.06%,差异无统计学意义(P>0.05).结论:光能量密度一致时,引发光功率密度对光固化复合树脂聚合收缩无显著影响,对树脂耐老化性能的影响与老化作用时间相关.%Objective:The purpose of this study was to investigate the effect of light power density on anti-aging property of composite resins. Methods: Three light curing modes with different power density (1. Mode A: low power density, 500 mW/cm2 for 22 s; 2. Mode B: high power density, 1 100 mW/ cm2 for 10 s; 3. Mode C: gradually enhanced power density, 0 ~650 mW/cm2 for 5 s + 1 100 mW/cm2 for 8.5 s) were used. The total energy density of these modes was all 11 J/cm2. Composite resin specimens were cured with three light curing modes respectively and divided into 4 groups randomly ( n= 15 ).Three

  6. Whitening and anti-aging effect of ginseng saponin nano and its safety evaluation%人参皂苷纳米乳的美白抗衰作用及其安全性评价

    Institute of Scientific and Technical Information of China (English)

    贾越光; 丁志英; 肖嘉婧; 张新然; 池季洪; 孙波

    2015-01-01

    目的:研究人参皂苷纳米乳的美白抗衰作用及其安全性。方法美白作用:用维生素C为对照药品,测定酪氨酸酶抑制率。抗衰老作用:建立D-半乳糖大鼠衰老模型,在大鼠颈背部皮肤上涂抹人参皂苷纳米乳及对照药品,每日2次,制备涂片,比较皮肤组织纤维的排列变化,测定DPPH自由基清除率。安全性评价:观察皮肤涂抹人参皂苷纳米乳及对照药品后,不同时间的皮肤变化情况。结果人参皂苷浓度为7.00 mg/mL时,酪氨酸酶抑制率顺序是,维生素C水溶液>人参皂苷纳米乳>人参皂苷水溶液;在衰老大鼠皮肤上涂抹人参皂苷纳米乳,切片结果显示,弹力纤维与皮肤表面较为平行,而未涂抹的对照组,弹力纤维排列较为曲折,与皮肤表面不平行;DPPH自由基清除率结果显示,维生素C水溶液>人参皂苷纳米乳>人参皂苷水溶液,且随着人参皂苷载药量的增大,人参皂苷纳米乳清除DPPH自由基的效率明显上升;安全性结果显示,皮肤涂抹人参皂苷纳米乳后,没有红肿、刺激等现象发生。结论人参皂苷纳米乳比人参皂苷水溶液具有更明显的美白抗衰作用,没有刺激,不会对皮肤造成损伤,安全可靠。%Objective To study on whitening and anti -aging effect of ginseng saponin and its safety.Methods Whitening effect:using vitamin C as the control drug, the inhibition rate of tyrosinase was determined.Anti senescence effect: the aging model of D-was established, and the DPPH was applied to the skin of the rat's neck, and the drug was prepared by 2 times a day.Skin safety evaluation: the skin changes of the skin of the ginseng saponins were observed after the skin was given ginseng saponin and control drugs.Results When the concentration of ginsenoside was 7 mg/mL, the inhibition rate order was, the water solution of vitamin C >ginsenoside nano >ginsenoside aqueous solution

  7. How to save Medicare: the anti-aging remedy.

    Science.gov (United States)

    Blagosklonny, Mikhail V

    2012-08-01

    The unprecedented progress in aging research has revealed that rapamycin, a clinically approved drug, is actually an anti-aging agent, which potentially could be employed to delay age-related diseases, thus extending healthy life span. The possibility of preventing diseases by staying young is remarkable in itself. At the same time this advance could save Medicare as we know it. Here I discuss how anti-aging interventions could solve otherwise intractable political problems without tax increases or curtailment of health care benefits.

  8. Hormesis-Based Anti-Aging Products: A Case Study of a Novel Cosmetic

    OpenAIRE

    Rattan, Suresh I.S.; Kryzch, Valérie; Schnebert, Sylvianne; Perrier, Eric; Nizard, Carine

    2012-01-01

    Application of hormesis in aging research and interventions is becoming increasingly attractive and successful. The reason for this is the realization that mild stress-induced activation of one or more stress response (SR) pathways, and its consequent stimulation of repair mechanisms, is effective in reducing the age-related accumulation of molecular damage. For example, repeated heat stress-induced synthesis of heat shock proteins has been shown to have a variety of anti-aging effects on gro...

  9. Rapalogs and mTOR inhibitors as anti-aging therapeutics.

    Science.gov (United States)

    Lamming, Dudley W; Ye, Lan; Sabatini, David M; Baur, Joseph A

    2013-03-01

    Rapamycin, an inhibitor of mechanistic target of rapamycin (mTOR), has the strongest experimental support to date as a potential anti-aging therapeutic in mammals. Unlike many other compounds that have been claimed to influence longevity, rapamycin has been repeatedly tested in long-lived, genetically heterogeneous mice, in which it extends both mean and maximum life spans. However, the mechanism that accounts for these effects is far from clear, and a growing list of side effects make it doubtful that rapamycin would ultimately be beneficial in humans. This Review discusses the prospects for developing newer, safer anti-aging therapies based on analogs of rapamycin (termed rapalogs) or other approaches targeting mTOR signaling.

  10. [Comment on the misappropriation of bibliographical references in science. The example of anti-aging medicine].

    Science.gov (United States)

    Cogan, E

    2015-01-01

    This work constitutes a argued analysis of the publication of the article of Hertoghe et al. "Anti aging medicine, a science based, essential medicine " whose full and unreviewed publication was forced in the framework of the Belgian law on the right of reply to an earlier publication entitled " Anti-Aging Medicine: Science or Marketing? ". We confirm the absence of scientific evidence on the effectiveness of hormonal treatments used in this approach by highlighting the different techniques allowing doctors who promote this approach to make believe in their effectiveness. This is clearly to mix in one sentence established truths and unproven facts, use references inappropriately especially by misappropriation of studies on groups of patients with hormone deficiency in order to justify treatment in healthy subjects, to ignore recent references undermining ancient literature, to betray the authors' conclusions. Our critical analysis is also considering compliance with the guidelines for integrity in scientific publications.

  11. Development and Evaluation of In-Vitro Antioxidant Potential and In-Vivo Anti-Ageing Activity of Polyherbal Formulation

    Directory of Open Access Journals (Sweden)

    Shinde VM

    2016-05-01

    Full Text Available Aging is indicated by a slow, gradual, structural and functional decline transformation that occurs at various levels of cells, tissues and organs. In human body, oxidative stress play major role in ageing process. Now a day, the free radical theory of ageing can help to understand the process of ageing and search for the effective anti ageing agents. Previous literature review of research has indicated that many of the traditional plants possess potent anti-ageing activity. Present study focuses on different theories of ageing and anti-ageing effects of polyherbal formulation (PHF of Emblica officinalis, Curcuma longa, Tribulus terrestris and Asparagus racemosus. Moreover, the phytochemical characterization and antioxidant potential of the extract was also measured by determining total phenolic contents, DPPH (2,2-diphenyl-β-picrylhydrazyl, reducing power assay which are estimated in in-vitro study. In-vivo anti-ageing activity performed by using D-galactose induced ageing model. Biochemical investigation was done for lipid peroxidation, lipofuscinogensis and total protein. The present study demonstrated that PHF have significant anti ageing capacity, safety and potential to demolish the oxidative stress in body.

  12. Molecular mechanisms for anti-aging by natural dietary compounds.

    Science.gov (United States)

    Pan, Min-Hsiung; Lai, Ching-Shu; Tsai, Mei-Ling; Wu, Jia-Ching; Ho, Chi-Tang

    2012-01-01

    Aging is defined as a normal decline in survival with advancing age; however, the recent researches have showed that physiological functions of the body change during the aging process. Majority of the changes are often subject to a higher risk of developing diseases, such as cardiovascular disease, type II diabetes, Alzheimer's disease, Parkinson's disease, as well as the dysregulated immune and inflammatory disorders. Aging process is controlled by a complicated and precise signaling network that involved in energy homeostasis, cellular metabolism and stress resistance. Over the past few decades, research in natural dietary compounds by various organism and animal models provides a new strategy for anti-aging. Natural dietary compounds act through a variety mechanisms to extend lifespan and prevent age-related diseases. This review summarizes the current understanding on signaling pathways of aging and knowledge and underlying mechanism of natural dietary compounds that provide potential application on anti-aging and improve heath in human.

  13. [Anti-aging medicine, a science-based, essential medicine].

    Science.gov (United States)

    Hertoghe, T; Lhermitte, M C; Poutet, B; Godefroit, C; Privé, D; Baneth, E; Everard, B; Hertoghe, T; Guery, G; Gadomski, A; Walraevens, A; Résimont, S; Wechoko, L; Seny, E; Vollon, K; Claeys, B

    2015-01-01

    Anti-aging medicine is booming. It enters more and more in the programs of universities. Its hormone and nutritional tests and therapies rely on numerous scientific studies, including double-blind placebo-controlled randomized studies. Its methods are often innovative to obtain more information or benefits with greater safety. The fundamental purpose of anti-aging medicine is to optimize health and the quality of life, and through this, make the physical appearance more youthful. Well-chosen and well-dosed these treatments should not increase the risk of age-related diseases such as cancer and cardiovascular diseases, but on the contrary decrease it by the preventive aspect of the treatments. Opponents to anti-aging medicine fail in collecting valid scientific arguments. Their insistence on maintaining a society of elderly people looking and feeling as elderly people rather than actively participating in searching for ways to attenuate aging is harmful to all who follow them, to themselves in the first place.

  14. Selective anti-cancer agents as anti-aging drugs.

    Science.gov (United States)

    Blagosklonny, Mikhail V

    2013-12-01

    Recent groundbreaking discoveries have revealed that IGF-1, Ras, MEK, AMPK, TSC1/2, FOXO, PI3K, mTOR, S6K, and NFκB are involved in the aging process. This is remarkable because the same signaling molecules, oncoproteins and tumor suppressors, are well-known targets for cancer therapy. Furthermore, anti-cancer drugs aimed at some of these targets have been already developed. This arsenal could be potentially employed for anti-aging interventions (given that similar signaling molecules are involved in both cancer and aging). In cancer, intrinsic and acquired resistance, tumor heterogeneity, adaptation, and genetic instability of cancer cells all hinder cancer-directed therapy. But for anti-aging applications, these hurdles are irrelevant. For example, since anti-aging interventions should be aimed at normal postmitotic cells, no selection for resistance is expected. At low doses, certain agents may decelerate aging and age-related diseases. Importantly, deceleration of aging can in turn postpone cancer, which is an age-related disease.

  15. Rapalogs in cancer prevention: anti-aging or anticancer?

    Science.gov (United States)

    Blagosklonny, Mikhail V

    2012-12-01

    Common cancer is an age-related disease. Slow aging is associated with reduced and delayed carcinogenesis. Calorie restriction (CR), the most studied anti-aging intervention, prevents cancer by slowing down the aging process. Evidence is emerging that CR decelerates aging by deactivating MTOR (Target of Rapamycin). Rapamycin and other rapalogs suppress cellular senescence, slow down aging and postpone age-related diseases including cancer. At the same time, rapalogs are approved for certain cancer treatments. Can cancer prevention be explained by direct targeting of cancer cells? Or does rapamycin prevent cancer indirectly through slowing down the aging process? Increasing evidence points to the latter scenario.

  16. Bioidentical hormones, menopausal women, and the lure of the "natural" in U.S. anti-aging medicine.

    Science.gov (United States)

    Fishman, Jennifer R; Flatt, Michael A; Settersten, Richard A

    2015-05-01

    In 2002, the Women's Health Initiative, a large-scale study of the safety of hormone replacement therapy (HRT) for women conducted in the United States, released results suggesting that use of postmenopausal HRT increased women's risks of stroke and breast cancer. In the years that followed, as rates of HRT prescription fell, another hormonal therapy rose in its wake: bioidentical hormone replacement therapy (BHRT). Anti-aging clinicians, the primary prescribers of BHRT, tout it as a safe and effective alternative to treat menopausal symptoms and, moreover, as a preventative therapy for age-related diseases and ailments. Through in-depth interviews with 31 U.S.-based anti-aging clinicians and 25 female anti-aging patients, we analyze attitudes towards BHRT. We illustrate how these attitudes reveal broader contemporary values, discourses, and discomforts with menopause, aging, and biomedicine. The attraction to and promise of BHRT is rooted in the idea that it is a "natural" therapy. BHRT is given both biomedical and embodied legitimacy by clinicians and patients because of its purported ability to become part of the body's "natural" processes. The normative assumption that "natural" is inherently "good" not only places BHRT beyond reproach, but transforms its use into a health benefit. The clinical approach of anti-aging providers also plays a role by validating patients' embodied experiences and offering a "holistic" solution to their symptoms, which anti-aging patients see as a striking contrast to their experiences with conventional biomedical health care. The perceived virtues of BHRT shed light on the rhetoric of anti-aging medicine and a deeply complicated relationship between conventional biomedicine, hormonal technologies, and women's bodies.

  17. Anti-aging herbal medicine--how and why can they be used in aging-associated neurodegenerative diseases?

    Science.gov (United States)

    Ho, Yuen-Shan; So, Kwok-Fai; Chang, Raymond Chuen-Chung

    2010-07-01

    Aging is a universal biological process that leads to progressive and deleterious changes in organisms. From ancient time, mankind has already interested in preventing and keeping ourselves young. Anti-aging study is certainly not a new research area. Nowadays, the meaning of anti-aging has been changed from simply prolonging lifespan to increasing health span, which emphasizes more on the quality of life. This is the concept of healthy aging and prevention of pathological aging, which is associated with diseases. Keeping our brain functions as in young age is an important task for neuroscientists to prevent aging-associated neurological disorders, such as Alzheimer's diseases (AD) and Parkinson's disease (PD). The causes of these diseases are not fully understood, but it is believed that these diseases are affected by multiple factors. Neurodegenerative diseases can be cross-linked with a number of aging-associated conditions. Based on this, a holistic approach in anti-aging research seems to be more reasonable. Herbal medicine has a long history in Asian countries. It is believed that many of the medicinal herbs have anti-aging properties. Recent studies have shown that some medicinal herbs are effective in intervention or prevention of aging-associated neurological disorders. In this review, we use wolfberry and ginseng as examples to elaborate the properties of anti-aging herbs. The characteristics of medicinal herbs, especially their applications in different disease stages (prevention and intervention) and multi-targets properties, allow them to be potential anti-aging intervention in prevention and treatment of the aging-associated neurological disorders.

  18. Anti-Aging Potential of Phytoextract Loaded-Pharmaceutical Creams for Human Skin Cell Longetivity

    Science.gov (United States)

    Jadoon, Saima; Karim, Sabiha; Asad, Muhammad Hassham Hassan Bin; Akram, Muhammad Rouf; Kalsoom Khan, Abida; Malik, Arif; Chen, Chunye; Murtaza, Ghulam

    2015-01-01

    The exposure to ultraviolet radiations (UVR) is the key source of skin sunburn; it may produce harmful entities, reactive oxygen species (ROS), leading to aging. The skin can be treated and protected from the injurious effects of ROS by using various pharmaceutical formulations, such as cream. Cream can be loaded with antioxidants to quench ROS leading to photo-protective effects. Moreover, modern medicines depend on ethnobotanicals for protection or treatment of human diseases. This review article summarizes various in vivo antioxidant studies on herbal creams loaded with phyto-extracts. These formulations may serve as cosmeceuticals to protect skin against injurious effects of UVR. The botanicals studied for dermatologic use in cream form include Acacia nilotica, Benincasa hispida, Calendula officinalis, Camellia sinensis, Camellia sinensis, Nelumbo nucifera, Capparis decidua, Castanea sativa, Coffea arabica, Crocus sativus, Emblica officinalis Gaertn, Foeniculum vulgare, Hippophae rhamnoides, Lithospermum erythrorhizon, Malus domestica, Matricaria chamomilla L., Moringa oleifera, Morus alba, Ocimum basilicum, Oryza sativa, Polygonum minus, Punica granatum, Silybum marianum, Tagetes erecta Linn., Terminalia chebula, Trigonella foenum-graecum, and Vitis vinifera. The observed anti-aging effects of cream formulations could be an outcome of a coordinating action of multiple constituents. Of numerous botanicals, the phenolic acids and flavonoids appear effective against UVR-induced damage; however the evidence-based studies for their anti-aging effects are still needed. PMID:26448818

  19. Anti-Aging Potential of Phytoextract Loaded-Pharmaceutical Creams for Human Skin Cell Longetivity

    Directory of Open Access Journals (Sweden)

    Saima Jadoon

    2015-01-01

    Full Text Available The exposure to ultraviolet radiations (UVR is the key source of skin sunburn; it may produce harmful entities, reactive oxygen species (ROS, leading to aging. The skin can be treated and protected from the injurious effects of ROS by using various pharmaceutical formulations, such as cream. Cream can be loaded with antioxidants to quench ROS leading to photo-protective effects. Moreover, modern medicines depend on ethnobotanicals for protection or treatment of human diseases. This review article summarizes various in vivo antioxidant studies on herbal creams loaded with phyto-extracts. These formulations may serve as cosmeceuticals to protect skin against injurious effects of UVR. The botanicals studied for dermatologic use in cream form include Acacia nilotica, Benincasa hispida, Calendula officinalis, Camellia sinensis, Camellia sinensis, Nelumbo nucifera, Capparis decidua, Castanea sativa, Coffea arabica, Crocus sativus, Emblica officinalis Gaertn, Foeniculum vulgare, Hippophae rhamnoides, Lithospermum erythrorhizon, Malus domestica, Matricaria chamomilla L., Moringa oleifera, Morus alba, Ocimum basilicum, Oryza sativa, Polygonum minus, Punica granatum, Silybum marianum, Tagetes erecta Linn., Terminalia chebula, Trigonella foenum-graecum, and Vitis vinifera. The observed anti-aging effects of cream formulations could be an outcome of a coordinating action of multiple constituents. Of numerous botanicals, the phenolic acids and flavonoids appear effective against UVR-induced damage; however the evidence-based studies for their anti-aging effects are still needed.

  20. Anti-Aging Potential of Phytoextract Loaded-Pharmaceutical Creams for Human Skin Cell Longetivity.

    Science.gov (United States)

    Jadoon, Saima; Karim, Sabiha; Bin Asad, Muhammad Hassham Hassan; Akram, Muhammad Rouf; Khan, Abida Kalsoom; Malik, Arif; Chen, Chunye; Murtaza, Ghulam

    2015-01-01

    The exposure to ultraviolet radiations (UVR) is the key source of skin sunburn; it may produce harmful entities, reactive oxygen species (ROS), leading to aging. The skin can be treated and protected from the injurious effects of ROS by using various pharmaceutical formulations, such as cream. Cream can be loaded with antioxidants to quench ROS leading to photo-protective effects. Moreover, modern medicines depend on ethnobotanicals for protection or treatment of human diseases. This review article summarizes various in vivo antioxidant studies on herbal creams loaded with phyto-extracts. These formulations may serve as cosmeceuticals to protect skin against injurious effects of UVR. The botanicals studied for dermatologic use in cream form include Acacia nilotica, Benincasa hispida, Calendula officinalis, Camellia sinensis, Camellia sinensis, Nelumbo nucifera, Capparis decidua, Castanea sativa, Coffea arabica, Crocus sativus, Emblica officinalis Gaertn, Foeniculum vulgare, Hippophae rhamnoides, Lithospermum erythrorhizon, Malus domestica, Matricaria chamomilla L., Moringa oleifera, Morus alba, Ocimum basilicum, Oryza sativa, Polygonum minus, Punica granatum, Silybum marianum, Tagetes erecta Linn., Terminalia chebula, Trigonella foenum-graecum, and Vitis vinifera. The observed anti-aging effects of cream formulations could be an outcome of a coordinating action of multiple constituents. Of numerous botanicals, the phenolic acids and flavonoids appear effective against UVR-induced damage; however the evidence-based studies for their anti-aging effects are still needed.

  1. Benefits of Anti-Aging Actives in Sunscreens

    Directory of Open Access Journals (Sweden)

    Karl Lintner

    2017-01-01

    Full Text Available Sunscreens are functional, utilitarian, cosmetic products. The criteria of purchase are different from those for skin care and make-up. Companies are trying to add glamour and value to basic sunscreens by incorporating “active” ingredients (other than UV filters into these formulas and by communicating about the additional benefits, be they anti-aging, moisturizing, firming, anti-wrinkle, etc. While some of these ideas of additional ingredients make sense as supplementary skin protection, some others do not afford much benefit in view of the infrequent application and short period of usage. The present article reviews some of these ideas and presents a few active ingredients that might be of value in such a context, even if substantiation of such additional claims in sunscreens is often lacking.

  2. Preparation, Component Analysis and Anti-Ageing Effect of Brain Active Peptide of Sucking Pig Brain%乳猪脑活性多肽的制备,成分分 析及实验动物抗脑老化研究

    Institute of Scientific and Technical Information of China (English)

    张文治; 苏心; 秦进喜; 张国福; 孔繁明; 吴剑涓; 只达石

    2001-01-01

    Objective: To study the preparation,component and anti-ageing effect of the brain active peptide of sucking pig brain. Methods: Through homogenate, degrease, heat, hydrolyze, centrifugation and ultrafiltration of sucking pig brain tissue, the brain active peptide was prepared. It was measured by physical and biochemical methods and its anti-ageing effect was co- mared compared with the expensive Cerebrolysin made in Austria. Results: The analysis of high pressure lipid chromato- graphy(HPLC) showed that both peak areas of the brain active peptide and Cerebrolysin were identical and some amino-acid contents of the brain active peptide were more than those of Cerebrolysin. Conclusion: The product of brain active peptide ac- cords with the PRC Hygiene Ministry standard about brain protein hydrolytic reagent injection 《WS-198(X-171)-97(1)》.%目的:制备乳猪脑活性多肽替代价格昂贵的进口脑活素,用于临床治疗。方法:乳猪脑组织经匀浆、脱 脂、加热、消化、离心、超滤,制备脑活性多肽,并与奥地利产脑活素对比进行理化检测,并用SAM-P/8早老鼠进行 脑活性多肽抗脑老化的研究。结果:高效液相色谱分析,乳猪脑活性多肽峰型分布与奥地利产脑活素基本一致。 氨基酸成分分析,一些氨基酸含量高于奥地利产脑活素。结论:制备的脑活性多肽注射液符合中华人民共和国卫 生部《WS198(X-171)-97(1)》有关脑蛋白水解物注射液部标准(试行)。

  3. OSTEOPOROSIS AND ALZHEIMER PATHOLOGY: ROLE OF CELLULAR STRESS RESPONSE AND HORMETIC REDOX SIGNALING IN AGING AND BONE REMODELING

    Directory of Open Access Journals (Sweden)

    Vittorio eCalabrese

    2014-06-01

    Full Text Available Alzheimer’s disease (AD as well as osteoporosis are multifactorial progressive degenerative disorders characterized by low parenchymal density and microarchitectural deterioration of tissue. Though not referred to as one of the major complications of AD, osteoporosis and hip fracture are commonly observed in patients with AD, however, the mechanisms underlying this association remain poorly understood. Reactive oxygen species (ROS are generally recognized as intracellular redox signaling molecules involved in the regulation of bone metabolism, including receptor activator of nuclear factor-kB ligand (RANKL-dependent osteoclast differentiation, but they also have cytotoxic effects that include peroxidation of lipids and oxidative damage to proteins and DNA. ROS formation, which is positively implicated in cellular stress response mechanisms, is a highly regulated process controlled by a complex network of intracellular signaling pathways which regulate life span across species including vitagenes which are genes involved in preserving cellular homeostasis during stressful conditions. Vitagenes encode for heat shock proteins (Hsp Hsp32, Hsp70, the thioredoxin and the sirtuin protein systems. Dietary antioxidants, have recently been demonstrated to be neuroprotective through the activation of hormetic pathways, including vitagenes. The hormetic dose–response, has the potential to affect significantly the design of pre-clinical studies and clinical trials as well as strategies for optimal patient dosing in the treatment of numerous diseases. Given the broad cytoprotective properties of the heat shock response there is now strong interest in discovering and developing pharmacological agents capable of inducing stress responses. Here we focus on possible signaling mechanisms involved in bone remodeling and activation of vitagenes resulting in enhanced defense against energy and stress resistance homeostasis dysruption with consequent impact on

  4. Principles and practice of hormetic treatment of aging and age-related diseases.

    Science.gov (United States)

    Rattan, Suresh Is

    2008-02-01

    Aging is characterized by stochastic accumulation of molecular damage, progressive failure of maintenance and repair, and consequent onset of age-related diseases. Applying hormesis in aging research and therapy is based on the principle of stimulation of maintenance and repair pathways by repeated exposure to mild stress. Studies on the beneficial biological effects of repeated mild heat shock on human cells in culture, and other studies on the anti-aging and life-prolonging effects of proxidants, hypergravity, irradiation and ethanol on cells and organisms suggest that hormesis as an antiaging and gerontomodulatory approach has a promising future. Its clinical applications include prevention and treatment of diabetes, cataract, osteoporosis, dementia and some cancers.

  5. Trade-offs between anti-aging dietary supplementation and exercise.

    Science.gov (United States)

    Mendelsohn, Andrew R; Larrick, James W

    2013-10-01

    In otherwise healthy adults, moderate aerobic exercise extends life span and likely health span by 2-6 years. Exercise improves blood sugar regulation, and resistance exercise increases or maintains muscle mass and is associated with improved cognitive function. On the other hand, evidence for anti-oxidant supplements increasing longevity in humans is lacking. On the contrary, transient hormetic increases in reactive oxygen species (ROS), for example, associated with exercise, are actually associated with increased mammalian health span and life span. Recent studies in humans suggest that anti-oxidants such as vitamins C, E, resveratrol, and acetyl-N-cysteine blunt the beneficial effects of exercise on glucose sensitivity and blood sugar regulation, likely through direct inhibition of ROS signaling. Alternately, other studies suggest that vitamin C has beneficial effects on exercise-associated dysfunction, inhibiting exercise-induced bronchioconstriction. These data suggest that there are tradeoffs between potential benefits and harm from anti-oxidant dietary supplementation. Specific biomolecular interactions for each antioxidant also will be important. Omega-3 (n-3) polyunsaturated fattty acids (PUFAs) have anti-inflammatory activity that is not mediated through direct ROS inhibition. Although data are limited in humans, n-3 PUFAs do not seem to blunt blood sugar regulatory benefits of aerobic exercise and actually increase anabolic activity in skeletal muscle. However, another kind of tradeoff may exist with PUFAs, at least for men. A recent large clinical trial demonstrates an association of omega-3 fatty acids blood levels with increased incidence of prostate cancer, especially aggressive prostate cancer. Together these results suggest that there are significant tradeoffs in the use of dietary supplementation for prevention and treatment of diseases associated with aging. Such tradeoffs may result from underlying intertwined homeostatic mechanisms. For most

  6. The natural phytochemical dehydroabietic acid is an anti-aging reagent that mediates the direct activation of SIRT1.

    Science.gov (United States)

    Kim, Juewon; Kang, Young-Gyu; Lee, Jee-young; Choi, Dong-hwa; Cho, Young-uk; Shin, Jae-Min; Park, Jun Seong; Lee, John Hwan; Kim, Wan Gi; Seo, Dae Bang; Lee, Tae Ryong; Miyamoto, Yusei; No, Kyoung Tai

    2015-09-05

    Dehydroabietic acid (DAA) is a naturally occurring diterpene resin acid of confers, such as pinus species (P. densiflora, P. sylvestris) and grand fir (Abies grandis), and it induces various biological actions including antimicrobial, antiulcer, and cardiovascular activities. The cellular targets that mediate these actions are largely unknown yet. In this report, we suggest that DAA is an anti-aging reagent. DAA has lifespan extension effects in Caenorhabditis elegans, prevents lipofuscin accumulation, and prevents collagen secretion in human dermal fibroblasts. We found that these anti-aging effects are primarily mediated by SIRT1 activation. Lifespan extension effects by DAA were ameliorated in sir-2.1 mutants and SIRT1 protein expression was increased, resulting in the deacetylation of SIRT1 target protein PGC-1α. Moreover, DAA binds directly to the SIRT1 protein independent of the SIRT1 substrate NAD(+) levels. Through a molecular docking study, we also propose a binding model for DAA-SIRT1. Taken together, our results demonstrate that the anti-aging effects are the first identified biological property of DAA and that the direct activation of SIRT1 enzymatic activity suggests the potential use of this natural diterpene, or related compounds, in age-related diseases or as a preventive reagent against the aging process.

  7. Application of rubber anti-aging agents in preventing coal spontaneous combustion

    Energy Technology Data Exchange (ETDEWEB)

    Yu, S.; Zhang, R.; Yang, Y.; Yang, H.; Fan, X.

    1999-07-01

    Based on the mechanism of rubber aging and action of anti-aging agents in preventing rubber aging, the free radical chain reaction mechanism of the oxidation of coal was discussed and compound stopping agents were introduced in the paper. The rubber anti-aging agents of aromatic amine are selected for the stopping agent of preventing coal spontaneous combustion. The stopping action of these anti-aging agents and common inorganic salt stopping agents for long flame coal in Gengcun Colliery, Yima city, Henan province in China is researched. The anti-aging agents for the long flame coal have the prominent efficiency and fine stopping action in later period, and inorganic stopping agents containing magnesium chloride salt for the long flame coal have the prominent efficiency in early period, but the compound stopping agents that are composed of the rubber anti-aging agents and magnesium chloride have longer life.

  8. Hormetic heat stress and HSF-1 induce autophagy to improve survival and proteostasis in C. elegans

    Science.gov (United States)

    Kumsta, Caroline; Chang, Jessica T.; Schmalz, Jessica; Hansen, Malene

    2017-01-01

    Stress-response pathways have evolved to maintain cellular homeostasis and to ensure the survival of organisms under changing environmental conditions. Whereas severe stress is detrimental, mild stress can be beneficial for health and survival, known as hormesis. Although the universally conserved heat-shock response regulated by transcription factor HSF-1 has been implicated as an effector mechanism, the role and possible interplay with other cellular processes, such as autophagy, remains poorly understood. Here we show that autophagy is induced in multiple tissues of Caenorhabditis elegans following hormetic heat stress or HSF-1 overexpression. Autophagy-related genes are required for the thermoresistance and longevity of animals exposed to hormetic heat shock or HSF-1 overexpression. Hormetic heat shock also reduces the progressive accumulation of PolyQ aggregates in an autophagy-dependent manner. These findings demonstrate that autophagy contributes to stress resistance and hormesis, and reveal a requirement for autophagy in HSF-1-regulated functions in the heat-shock response, proteostasis and ageing. PMID:28198373

  9. Are “Anti-Aging Medicine” and “Successful Aging” Two Sides of the Same Coin? Views of Anti-Aging Practitioners

    Science.gov (United States)

    2013-01-01

    Objectives. This article analyzes data from interviews with anti-aging practitioners to evaluate how their descriptions of the work they do, their definitions of aging, and their goals for their patients intersect with gerontological views of “successful aging.” Method. Semistructured interviews were conducted with a sample of 31 anti-aging practitioners drawn from the directory of the American Academy for Anti-Aging Medicine. Results. Qualitative analysis of the transcripts demonstrate that practitioners’ descriptions of their goals, intentionally or unintentionally, mimic the dominant models of “successful aging.” These include lowered risk of disease and disability, maintenance of high levels of mental and physical function, and continuing social engagement. Yet, the means and modes of achieving these goals differ markedly between the two groups, as do the messages that each group puts forth in defending their positions. Discussion. Anti-aging practitioners’ adoption of the rhetoric of successful aging reflects the success of successful aging models in shaping popular conceptions of what aging is and an ethos of management and control over the aging process. The overlap between anti-aging and successful aging rhetoric also highlights some of the most problematic social, cultural, and economic consequences of efforts made to reconceptualize old age. PMID:24022620

  10. The Research on Mechanism of Anti-aging by Moxibustion%灸法抗衰老机制研究概况

    Institute of Scientific and Technical Information of China (English)

    潘亚辉; 杨继国

    2016-01-01

    古今文献均证实艾灸在延缓衰老方面有确切作用。当前对灸法抗衰老的研究日益增多,多侧重对其机制的探索。通过查阅灸法抗衰老的现代文献,从实验研究、临床研究两方面综述了灸法在抗衰老机制方面的研究进展,为推广艾灸抗衰老的临床应用提供参考依据。参考文献34篇。%Ancient and modern literature have proved that moxibustion has a definite anti-aging effect. Nowadays,the research on anti-aging of moxibustion is increasing,usually focusing on the underlying mecha-nism. Through reviewing the modern literature on anti-aging using moxibustion,the development of research on anti-aging mechanism by moxibustion is studied retrospectively from experimental research and clinical research. It will provide a research basis for clinical application of moxibustion for anti-aging. The references include 34 papers.

  11. Berberine protects against 6-OHDA-induced neurotoxicity in PC12 cells and zebrafish through hormetic mechanisms involving PI3K/AKT/Bcl-2 and Nrf2/HO-1 pathways

    Directory of Open Access Journals (Sweden)

    Chao Zhang

    2017-04-01

    Full Text Available Berberine (BBR is a renowned natural compound that exhibits potent neuroprotective activities. However, the cellular and molecular mechanisms are still unclear. Hormesis is an adaptive mechanism generally activated by mild oxidative stress to protect the cells from further damage. Many phytochemicals have been shown to induce hormesis. This study aims to investigate whether the neuroprotective activity of BBR is mediated by hormesis and the related signaling pathways in 6-OHDA-induced PC12 cells and zebrafish neurotoxic models. Our results demonstrated that BBR induced a typical hormetic response in PC12 cells, i.e. low dose BBR significantly increased the cell viability, while high dose BBR inhibited the cell viability. Moreover, low dose BBR protected the PC12 cells from 6-OHDA-induced cytotoxicity and apoptosis, whereas relatively high dose BBR did not show neuroprotective activity. The hormetic and neuroprotective effects of BBR were confirmed to be mediated by up-regulated PI3K/AKT/Bcl-2 cell survival and Nrf2/HO-1 antioxidative signaling pathways. In addition, low dose BBR markedly mitigated the 6-OHDA-induced dopaminergic neuron loss and behavior movement deficiency in zebrafish, while high dose BBR only slightly exhibited neuroprotective activities. These results strongly suggested that the neuroprotection of BBR were attributable to the hormetic mechanisms via activating cell survival and antioxidative signaling pathways.

  12. Hormetic response triggers multifaceted anti-oxidant strategies in immature king penguins (Aptenodytes patagonicus).

    Science.gov (United States)

    Rey, Benjamin; Dégletagne, Cyril; Bodennec, Jacques; Monternier, Pierre-Axel; Mortz, Mathieu; Roussel, Damien; Romestaing, Caroline; Rouanet, Jean-Louis; Tornos, Jeremy; Duchamp, Claude

    2016-08-01

    Repeated deep dives are highly pro-oxidative events for air-breathing aquatic foragers such as penguins. At fledging, the transition from a strictly terrestrial to a marine lifestyle may therefore trigger a complex set of anti-oxidant responses to prevent chronic oxidative stress in immature penguins but these processes are still undefined. By combining in vivo and in vitro approaches with transcriptome analysis, we investigated the adaptive responses of sea-acclimatized (SA) immature king penguins (Aptenodytes patagonicus) compared with pre-fledging never-immersed (NI) birds. In vivo, experimental immersion into cold water stimulated a higher thermogenic response in SA penguins than in NI birds, but both groups exhibited hypothermia, a condition favouring oxidative stress. In vitro, the pectoralis muscles of SA birds displayed increased oxidative capacity and mitochondrial protein abundance but unchanged reactive oxygen species (ROS) generation per g tissue because ROS production per mitochondria was reduced. The genes encoding oxidant-generating proteins were down-regulated in SA birds while mRNA abundance and activity of the main antioxidant enzymes were up-regulated. Genes encoding proteins involved in repair mechanisms of oxidized DNA or proteins and in degradation processes were also up-regulated in SA birds. Sea life also increased the degree of fatty acid unsaturation in muscle mitochondrial membranes resulting in higher intrinsic susceptibility to ROS. Oxidative damages to protein or DNA were reduced in SA birds. Repeated experimental immersions of NI penguins in cold-water partially mimicked the effects of acclimatization to marine life, modified the expression of fewer genes related to oxidative stress but in a similar way as in SA birds and increased oxidative damages to DNA. It is concluded that the multifaceted plasticity observed after marine life may be crucial to maintain redox homeostasis in active tissues subjected to high pro-oxidative pressure

  13. 单细胞海藻提取物对人脐带间充质干细胞的抗衰老作用∗%Anti-aging effects of marine phytoplankton on human umbilical cord de-rived mesenchymal stem cells

    Institute of Scientific and Technical Information of China (English)

    姚宁; 关方霞; 马珊珊; 崔渊博; 渠瑞娜; 王欣欣; 孟楠; 宋及时; 赵云; 高军

    2015-01-01

    Aim: To explore the anti-aging effects of marine phytoplankton(MPPT) on human umbilical cord derived mesenchymal stem cells(hUC-MSCs). Methods: hUC-MSCs were isolated from umbilical cords of mature healthy newbo-rns delivered by caesarean section, and then cultured and passaged, while the morphology was observed under microscope. The passage 3, 10, and 15 cells were collected and the mRNA expressions of p16, p21, p53, PCNA, and Sirt2 in different passages of hUC-MSCs were detected by qRT-PCR. CCK-8 assay was performed to detect the effect of MPPT on cell prolif-eration and flow cytometry was used to detect cell cycle and apoptosis in the passage 15 cells. β-galactosidase staining was used to detect the effect of MPPT on cell senescence, and the effect of MPPT on the mRNA expressions of p16, p21, p53, PCNA, and Sirt2 of hUC-MSCs were detected by qRT-PCR. Results: The cell morphology of passage 3 was fusiform and easily adherent, however, the passage 15 hUC-MSCs were flat and poorly adherent. The mRNA expressions of PCNA and Sirt2 reduced gradually along with passaging(F = 76. 944,25. 649,P < 0. 001), while those of p16, p21, and p53 in-creased(F = 46. 714,77. 645,845. 676, P < 0. 001). Compared with the passage 15 cells, 1 g/ L MPPT could promote the proliferation, induce cells into S phase, inhibit apoptosis of the passage 15 hUC-MSCs(P < 0. 05), decrease the positive rate of β-galactosidase positive staining cells(F = 773. 557,P < 0. 001), and increase the mRNA expressions of PCNA and Sirt2,while decrease the mRNA expressions of p16, p21, and p53(P < 0. 05). Conclusion: MPPT has anti-aging effects on hUC-MSCs, and the mechanism is partly through regulating the expressions of p16, p21, p53, PCNA, and Sirt2.%目的::探讨单细胞海藻(MPPT)提取物对人脐带间充质干细胞(hUC-MSCs)的抗衰老作用。方法:取剖宫产新生儿脐带,分离、传代培养 hUC-MSCs,显微镜下观察细胞形态,收集第3、10、15代细胞,qRT-PCR

  14. Curcumin induces stress response and hormetically modulates wound healing ability of human skin fibroblasts undergoing ageing in vitro.

    Science.gov (United States)

    Demirovic, Dino; Rattan, Suresh I S

    2011-10-01

    Wound healing becomes impaired in several diseases and during ageing. A commonly used model for the study of wound healing is a scratched monolayer of cells in vitro, which is convenient for the analysis of the cellular and molecular changes occurring during the two phases of wound healing, namely cell migration and cell proliferation. Cell migration, which is the primary event to occur during initial wound healing, is inversely dependent on the number of focal adhesions (FA) that attach cells to the extracellular matrix. Here we report that the number of FA, measured by determining the levels of FA-proteins paxillin and talin, increase with increasing population doubling level of the serially passaged normal adult skin fibroblasts, and that this increase may account for the age-related slowing down of wound healing in vitro. We also report that curcumin, a component of the widely used spice turmeric, modulates wound healing in vitro in a biphasic dose response manner, being stimulatory at low doses (between 1 and 5 μM), and inhibitory at higher doses. Furthermore, our results show that the hormetic effects of low levels of curcumin are achieved by virtue of it being a hormetin in terms of the induction of stress response pathways, including Nrf2 and HO-1 in human cells.

  15. Xenohormetic and anti-aging activity of secoiridoid polyphenols present in extra virgin olive oil

    Science.gov (United States)

    Menendez, Javier A.; Joven, Jorge; Aragonès, Gerard; Barrajón-Catalán, Enrique; Beltrán-Debón, Raúl; Borrás-Linares, Isabel; Camps, Jordi; Corominas-Faja, Bruna; Cufí, Sílvia; Fernández-Arroyo, Salvador; Garcia-Heredia, Anabel; Hernández-Aguilera, Anna; Herranz-López, María; Jiménez-Sánchez, Cecilia; López-Bonet, Eugeni; Lozano-Sánchez, Jesús; Luciano-Mateo, Fedra; Martin-Castillo, Begoña; Martin-Paredero, Vicente; Pérez-Sánchez, Almudena; Oliveras-Ferraros, Cristina; Riera-Borrull, Marta; Rodríguez-Gallego, Esther; Quirantes-Piné, Rosa; Rull, Anna; Tomás-Menor, Laura; Vazquez-Martin, Alejandro; Alonso-Villaverde, Carlos; Micol, Vicente; Segura-Carretero, Antonio

    2013-01-01

    Aging can be viewed as a quasi-programmed phenomenon driven by the overactivation of the nutrient-sensing mTOR gerogene. mTOR-driven aging can be triggered or accelerated by a decline or loss of responsiveness to activation of the energy-sensing protein AMPK, a critical gerosuppressor of mTOR. The occurrence of age-related diseases, therefore, reflects the synergistic interaction between our evolutionary path to sedentarism, which chronically increases a number of mTOR activating gero-promoters (e.g., food, growth factors, cytokines and insulin) and the “defective design” of central metabolic integrators such as mTOR and AMPK. Our laboratories at the Bioactive Food Component Platform in Spain have initiated a systematic approach to molecularly elucidate and clinically explore whether the “xenohormesis hypothesis,” which states that stress-induced synthesis of plant polyphenols and many other phytochemicals provides an environmental chemical signature that upregulates stress-resistance pathways in plant consumers, can be explained in terms of the reactivity of the AMPK/mTOR-axis to so-called xenohormetins. Here, we explore the AMPK/mTOR-xenohormetic nature of complex polyphenols naturally present in extra virgin olive oil (EVOO), a pivotal component of the Mediterranean style diet that has been repeatedly associated with a reduction in age-related morbid conditions and longer life expectancy. Using crude EVOO phenolic extracts highly enriched in the secoiridoids oleuropein aglycon and decarboxymethyl oleuropein aglycon, we show for the first time that (1) the anticancer activity of EVOO secoiridoids is related to the activation of anti-aging/cellular stress-like gene signatures, including endoplasmic reticulum (ER) stress and the unfolded protein response, spermidine and polyamine metabolism, sirtuin-1 (SIRT1) and NRF2 signaling; (2) EVOO secoiridoids activate AMPK and suppress crucial genes involved in the Warburg effect and the self-renewal capacity of

  16. Anti-Oxidant, Anti-Aging, and Anti-Melanogenic Properties of the Essential Oils from Two Varieties of Alpinia zerumbet.

    Science.gov (United States)

    Tu, Pham Thi Be; Tawata, Shinkichi

    2015-09-14

    Here, we investigated the anti-oxidant and anti-aging effects of essential oils (EOs) from the leaves of Alpinia zerumbet (tairin and shima) in vitro and anti-melanogenic effects in B16F10 melanoma cells. The anti-oxidant activities were performed with 2,2-diphenyl-1-picrylhydrazyl (DPPH); 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS); nitric oxide; singlet oxygen; hydroxyl radical scavenging; and xanthine oxidase. The inhibitory activities against collagenase, elastase, hyaluronidase, and tyrosinase were employed for anti-aging. The anti-melanogenic was assessed in B16F10 melanoma cells by melanin synthesis and intracellular tyrosinase inhibitory activity. The volatile chemical composition of the essential oil was analyzed with gas chromatography-mass spectrometry (GC/MS). The EO was a complex mixture mainly consisting of monoterpenes and sesquiterpenes. The results revealed that tairin and shima EOs showed strong anti-oxidant activities against DPPH and nitric oxide, hydroxyl radical scavenging activity, and xanthine oxidase inhibition. Compared to shima EO; tairin EO exhibited strong anti-aging activity by inhibiting collagenase, tyrosinase, hyaluronidase, and elastase (IC50 = 11 ± 0.1; 25 ± 1.2; 83 ± 1.6; and 213 ± 2 μg/mL, respectively). Both EOs inhibited intracellular tyrosinase activity; thus, reducing melanin synthesis. These results suggest that tairin EO has better anti-oxidant/anti-aging activity than shima EO, but both are equally anti-melanogenic.

  17. Group-based differences in anti-aging bias among medical students.

    Science.gov (United States)

    Ruiz, Jorge G; Andrade, Allen D; Anam, Ramanakumar; Taldone, Sabrina; Karanam, Chandana; Hogue, Christie; Mintzer, Michael J

    2015-01-01

    Medical students (MS) may develop ageist attitudes early in their training that may predict their future avoidance of caring for the elderly. This study sought to determine MS' patterns of explicit and implicit anti-aging bias, intent to practice with older people and using the quad model, the role of gender, race, and motivation-based differences. One hundred and three MS completed an online survey that included explicit and implicit measures. Explicit measures revealed a moderately positive perception of older people. Female medical students and those high in internal motivation showed lower anti-aging bias, and both were more likely to intend to practice with older people. Although the implicit measure revealed more negativity toward the elderly than the explicit measures, there were no group differences. However, using the quad model the authors identified gender, race, and motivation-based differences in controlled and automatic processes involved in anti-aging bias.

  18. [Caloric restriction in primates: how efficient as an anti-aging approach?].

    Science.gov (United States)

    Marchal, Julia; Perret, Martine; Aujard, Fabienne

    2012-12-01

    Caloric restriction (CR) is the only non-genetic intervention known to date to slow the onset of age-related diseases and increase average and maximum lifespan in several species. Its interest is continually growing, particularly for the identification of mechanisms involved in increasing longevity. Unlike studies in invertebrate and rodent models have provided some indication about the mechanisms of the CR, the efficacy of CR as an anti-aging protocol in primates has not yet been fully established. In this review we present the advantages of using non human primates as relevant models to the study of human aging in general and specifically in the context of therapeutic interventions applicable to humans, such as CR. Through the longitudinal findings in the Grey Mouse Lemur (Microcebus murinus), we stress the importance of primate studies in the context of research on aging and their potential to advance the development of molecules which can mimic the beneficial effects of CR, already observed in some species, without imposing a reduced calorie diet.

  19. Testosterone increases renal anti-aging klotho gene expression via the androgen receptor-mediated pathway.

    Science.gov (United States)

    Hsu, Shih-Che; Huang, Shih-Ming; Lin, Shih-Hua; Ka, Shuk-Man; Chen, Ann; Shih, Meng-Fu; Hsu, Yu-Juei

    2014-12-01

    Gender is known to be associated with longevity and oestrogen administration induced longevity-associated gene expression is one of the potential mechanisms underlying the benefits of oestrogen on lifespan, whereas the role of testosterone in the regulation of longevity-associated gene expressions remains largely unclear. The klotho gene, predominantly expressed in the kidney, has recently been discovered to be an aging suppressor gene. In the present study, we investigated the regulatory effects of testosterone on renal klotho gene expression in vivo and in vitro. In testosterone-administered mouse kidney and NRK-52E cells, increased klotho expression was accompanied by the up-regulation of the nuclear androgen receptor (AR). Overexpression of AR enhanced the expression of klotho mRNA and protein. Conversely, testosterone-induced klotho expression was attenuated in the presence of flutamide, an AR antagonist. A reporter assay and a chromatin immunoprecipitation (ChIP) assay demonstrated that AR directly binds to the klotho promoter via androgen response elements (AREs) which reconfirmed its importance for AR binding via the element mutation. In summary, our study demonstrates that testosterone up-regulates anti-aging klotho together with AR expression in the kidney in vivo and in vitro by recruiting AR on to the AREs of the klotho promoter.

  20. Voluntary exercise promotes beneficial anti-aging mechanisms in SAMP8 female brain.

    Science.gov (United States)

    Bayod, Sergi; Guzmán-Brambila, Carolina; Sanchez-Roige, Sandra; Lalanza, Jaume F; Kaliman, Perla; Ortuño-Sahagun, Daniel; Escorihuela, Rosa M; Pallàs, Mercè

    2015-02-01

    Regular physical exercise mediates health and longevity promotion involving Sirtuin 1 (SIRT1)-regulated pathways. The anti-aging activity of SIRT1 is achieved, at least in part, by means of fine-tuning the adenosine monophosphate (AMP)-activated protein kinase (AMPK) pathway by preventing the transition of an originally pro-survival program into a pro-aging mechanism. Additionally, SIRT1 promotes mitochondrial function and reduces the production of reactive oxygen species (ROS) through regulating peroxisome proliferator-activated receptor γ coactivator 1α (PGC-1α), the master controller of mitochondrial biogenesis. Here, by using senescence-accelerated mice prone 8 (SAMP8) as a model for aging, we determined the effect of wheel-running as a paradigm for long-term voluntary exercise on SIRT1-AMPK pathway and mitochondrial functionality measured by oxidative phosphorylation (OXPHOS) complex content in the hippocampus and cortex. We found differential activation of SIRT1 in both tissues and hippocampal-specific activation of AMPK. These findings correlated well with significant changes in OXPHOS in the hippocampal, but not in the cerebral cortex, area. Collectively, the results revealed greater benefits of the exercise in the wheel-running intervention in a murine model of senescence, which was directly related with mitochondrial function and which was mediated through the modulation of SIRT1 and AMPK pathways.

  1. Safety and efficacy of antioxidants-loaded nanoparticles for an anti-aging application.

    Science.gov (United States)

    Felippi, Cândice C; Oliveira, Dileusa; Ströher, Alessandra; Carvalho, Anderson R; Van Etten, Eliana A M Aquino; Bruschi, Márcia; Raffin, Renata P

    2012-04-01

    The aim of this work was to perform a pilot study on the safety and efficacy of nanoparticle formulation for cosmetic application. The encapsulated actives in the nanoparticles were a blend of coenzyme Q10, retinyl palmitate, tocopheryl acetate, grape seed oil and linseed oil. The nanoparticle suspension was characterized in terms of pH and particle size. For the safety assessment, alternative methods as cytotoxicity and HET CAM were used. The clinical skin compatibility tests were also performed. The efficacy was evaluated in healthy volunteers presenting different degrees of periorbital wrinkles. Skin hydration was performed by corneometry. The nanoparticles presented narrow size around 140 nm and pH close to neutral and were suitable to cutaneous application. The alternative tests demonstrated that the nanoparticles did not present potential to induce skin irritant effects, cytotoxicity or generate oxidative stress. The clinical assays confirmed the in vitro results, demonstrating the safety of the nanoparticles, which were not irritant, sensitizing and comedogenic. Furthermore, the exposure to UVA light did not cause photoxicity. Regarding the efficacy, nanoparticles presented significant reduction in wrinkle degree after 21 days of application compared to the control. The volunteers could differentiate the nanoparticles and the control product by means of subjective analyses. In conclusion, the nanoparticles containing antioxidant actives were safe for topical use and presented anti-aging activity in vivo and are suitable to be used as cosmetic ingredient.

  2. Colonization of later life? Laypersons' and users' agency regarding anti-aging medicine in Germany.

    Science.gov (United States)

    Schweda, Mark; Pfaller, Larissa

    2014-10-01

    Anti-aging medicine is regarded as a significant trend in contemporary Western societies. Foucauldian gerontology provides some of the dominant theoretical perspectives on this trend in social and cultural theory. Proceeding from its interpretation and critique of anti-aging in terms of medicalization and responsibilization of aging, we explore by means of qualitative socio-empirical research how interested laypersons as well as non-professional users in the German context actually perceive of and deal with the medical claims and moral imperatives surrounding anti-aging medicine. The study is based on 12 focus groups and 20 narrative interviews (96 participants all included). They were conducted in Germany between 2011 and 2012, and analyzed by qualitative content analysis as well as comparative sequence analysis. The empirical findings indicate that in everyday life, interested laypersons and non-professional users employ different strategies for dealing with anti-aging products and services, corresponding to different degrees of affirmation and rejection. Four strategies could be identified: (a) medical optimism, (b) preventive maximalism, (c) ritualized well-being, and (d) considerate rejection. Also, each type was problematized and arguments against it were expressed. Overall, these findings show how our participants develop viable strategies to put the relevance of medical knowledge and moral imperatives for their own lives into perspective. This sheds light on laypersons' and users' agency-that is, their active role and deliberative space in the uptake, adaptation, and integration of anti-aging into their personal life. These empirical findings contribute to an enriched picture of the actual practice of anti-aging in concrete national and socio-cultural settings. This can help to differentiate the evaluation and thus make its critique more context sensitive, adequate, and targeted.

  3. Overview of beverages with anti-aging functions in Chinese market.

    Science.gov (United States)

    Yang, Fan; Song, Jie; Liang, Ming; Ma, Fangli; Mao, Xinliang; Ma, Chung Wah; Zhang, Wanwan; Huang, Zebo

    2014-04-01

    Anti-aging Chinese medicines have been used in traditional beverages to promote health and prevent diseases. Interestingly, these functional beverages may be used differently between men and women, reflecting the "yin-yang" philosophy of Chinese medicine. Modern studies have revealed that some dietary natural products can slow aging in model organisms, and functional beverages containing such products have recently emerged in Chinese market, challenging the dominance of traditional functional beverages. Here we summarize both traditional anti-aging herbs and modern natural dietary compounds currently used in functional beverages in China, and also briefly outline the underlying mechanisms of the beverages in slowing aging process.

  4. Anti-aging properties of Ribes fasciculatum in Caenorhabditis elegans.

    Science.gov (United States)

    Jeon, Hoon; Cha, Dong Seok

    2016-05-01

    The present study investigated the effects and underlying mechanism of ethylacetate fraction of Ribes fasciculatum (ERF) on the lifespan and stress tolerance using a Caenorhabditis elegans model. The longevity activity of ERF was determined by lifespan assay under normal culture condition. The survival rate of nematodes under various stress conditions was assessed to validate the effects of ERF on the stress tolerance. To determine the antioxidant potential of ERF, the superoxide dismutase (SOD) activities and intracellular reactive oxygen species (ROS) levels were investigated. The ERF-mediated change in SOD-3 expression was examined using GFP-expressing transgenic strain. The effects of ERF on the aging-related factors were investigated by reproduction assay and pharyngeal pumping assay. The intestinal lipofuscin levels of aged nematodes were also measured. The mechanistic studies were performed using selected mutant strains. Our results indicated that ERF showed potent lifespan extension effects on the wild-type nematode under both normal and various stress conditions. The ERF treatment also enhanced the activity and expression of superoxide dismutase (SOD) and attenuated the intracellular ROS levels. Moreover, ERF-fed nematodes showed decreased lipofuscin accumulation, indicating ERF might affect age-associated changes in C. elegans. The results of mechanistic studies indicated that there was no significant lifespan extension in ERF-treated daf-2, age-1, sir-2.1, and daf-16 null mutants, suggesting that they were involved in ERF-mediated lifespan regulation. In conclusion, R. fasciculatum confers increased longevity and stress resistance in C. elegans via SIR-2.1-mediated DAF-16 activation, dependent on the insulin/IGF signaling pathway.

  5. Metformin: do we finally have an anti-aging drug?

    Science.gov (United States)

    Anisimov, Vladimir N

    2013-11-15

    Studies in mammals have demonstrated that hyperglycemia and hyperinsulinemia are important factors in aging and cancer. Inactivation of insulin/insulin-like signaling increases lifespan in nematodes, fruit flies, and mice. Life-prolonging effects of caloric restriction are in part due to reduction in IGF-1, insulin, and glucose levels. Antidiabetic biguanides such as metformin, which reduce hyperglycemia and hyperinsulinemia by decreasing insulin resistance, extend lifespan, and inhibit carcinogenesis in rodents. Will antidiabetic biguanides increase lifespan in humans?

  6. Current status of auditory aging and anti-aging research.

    Science.gov (United States)

    Ruan, Qingwei; Ma, Cheng; Zhang, Ruxin; Yu, Zhuowei

    2014-01-01

    The development of presbycusis, or age-related hearing loss, is determined by a combination of genetic and environmental factors. The auditory periphery exhibits a progressive bilateral, symmetrical reduction of auditory sensitivity to sound from high to low frequencies. The central auditory nervous system shows symptoms of decline in age-related cognitive abilities, including difficulties in speech discrimination and reduced central auditory processing, ultimately resulting in auditory perceptual abnormalities. The pathophysiological mechanisms of presbycusis include excitotoxicity, oxidative stress, inflammation, aging and oxidative stress-induced DNA damage that results in apoptosis in the auditory pathway. However, the originating signals that trigger these mechanisms remain unclear. For instance, it is still unknown whether insulin is involved in auditory aging. Auditory aging has preclinical lesions, which manifest as asymptomatic loss of periphery auditory nerves and changes in the plasticity of the central auditory nervous system. Currently, the diagnosis of preclinical, reversible lesions depends on the detection of auditory impairment by functional imaging, and the identification of physiological and molecular biological markers. However, despite recent improvements in the application of these markers, they remain under-utilized in clinical practice. The application of antisenescent approaches to the prevention of auditory aging has produced inconsistent results. Future research will focus on the identification of markers for the diagnosis of preclinical auditory aging and the development of effective interventions.

  7. Enhanced Antioxidant Capacity and Anti-Ageing Biomarkers after Diet Micronutrient Supplementation

    Directory of Open Access Journals (Sweden)

    Aneta Balcerczyk

    2014-09-01

    Full Text Available A growing number of studies confirm an important effect of diet, lifestyle and physical activity on health status, the ageing process and many metabolic disorders. This study focuses on the influence of a diet supplement, NucleVital®Q10 Complex, on parameters related to redox homeostasis and ageing. An experimental group of 66 healthy volunteer women aged 35–55 supplemented their diet for 12 weeks with the complex, which contained omega-3 acids (1350 mg/day, ubiquinone (300 mg/day, astaxanthin (15 mg/day, lycopene (45 mg/day, lutein palmitate (30 mg/day, zeaxanthine palmitate (6 mg/day, L-selenomethionine (330 mg/day, cholecalciferol (30 µg/day and α-tocopherol (45 mg/day. We found that NucleVital®Q10 Complex supplementation significantly increased total antioxidant capacity of plasma and activity of erythrocyte superoxide dismutase, with slight effects on oxidative stress biomarkers in erythrocytes; MDA and 4-hydroxyalkene levels. Apart from the observed antioxidative effects, the tested supplement also showed anti-ageing activity. Analysis of expression of SIRT1 and 2 in PBMCs showed significant changes for both genes on a mRNA level. The level of telomerase was also increased by more than 25%, although the length of lymphocyte telomeres, determined by RT-PCR, remained unchanged. Our results demonstrate beneficial effects concerning the antioxidant potential of plasma as well as biomarkers related to ageing even after short term supplementation of diet with NucleVital®Q10 Complex.

  8. Chitin-Hyaluronan Nanoparticles: A Multifunctional Carrier to Deliver Anti-Aging Active Ingredients through the Skin

    Directory of Open Access Journals (Sweden)

    Pierfrancesco Morganti

    2014-07-01

    Full Text Available The paper describes the process to produce Chitin Nanofibril-Hyaluronan nanoparticles (CN-HA, showing their ability to easily load active ingredients, facilitate penetration through the skin layers, and increase their effectiveness and safety as an anti-aging agent. Size and characterization of CN-HA nanoparticles were determined by Scanning Electron Microscopy (SEM and Zetasizer, while encapsulation efficiency and loading capacity of the entrapped ingredients were controlled by chromatographic and spectrophotometric methods. Safeness was evidenced on fibroblasts and keratinocytes culture viability by the MTT (Methylthiazol assay; anti-aging activity was evaluated in vitro measuring antioxidant capacity, anti-collagenase activity, and metalloproteinase and pro-inflammatory release; efficacy was shown in vivo by a double-blind vehicle-controlled study for 60 days on 60 women affected by photo-aging. In addition, the CN-HA nanoparticles have shown interesting possibility to be used as active ingredients, for designing and making advanced medication by the electrospinning technology, as well as to produce transparent films for food packaging, by the casting method, and can be used also in their dry form as tissues or films without adding preservatives. These unusual CN-HA nanoparticles obtained from the use of raw materials of waste origin may offer an unprecedented occasion for making innovative products, ameliorating the quality of life, reducing pollution and safeguarding the environment’s integrity.

  9. Schisandrin B as a Hormetic Agent for Preventing Age-Related Neurodegenerative Diseases

    Directory of Open Access Journals (Sweden)

    Philip Y. Lam

    2012-01-01

    Full Text Available Oxidative stress and mitochondrial dysfunction have been implicated in the pathogenesis of neurodegenerative diseases, with the latter preceding the appearance of clinical symptoms. The energy failure resulting from mitochondrial dysfunction further impedes brain function, which demands large amounts of energy. Schisandrin B (Sch B, an active ingredient isolated from Fructus Schisandrae, has been shown to afford generalized tissue protection against oxidative damage in various organs, including the brain, of experimental animals. Recent experimental findings have further demonstrated that Sch B can protect neuronal cells against oxidative challenge, presumably by functioning as a hormetic agent to sustain cellular redox homeostasis and mitoenergetic capacity in neuronal cells. The combined actions of Sch B offer a promising prospect for preventing or possibly delaying the onset of neurodegenerative diseases, as well as enhancing brain health.

  10. In Search for Anti-Aging Strategy: Can We Rejuvenate Our Aging Stem Cells?

    Directory of Open Access Journals (Sweden)

    Anna Meiliana

    2015-08-01

    Full Text Available BACKGROUND: Recent evidence suggested that we grow old partly because of our stem cells grow old as a result of mechanisms that suppress the development of cancer over a lifetime. We believe that a further, more precise mechanistic understanding of this process will be required before this knowledge can be translated into human anti-aging therapies. CONTENT: A diminished capacity to maintain tissue homeostasis is a central physiological characteristic of aging. As stem cells regulate tissue homeostasis, depletion of stem cell reserves and/or diminished stem cell function have been postulated to contribute to aging. It has further been suggested that accumulated DNA damage could be a principal mechanism underlying age-dependent stem cell decline. It is interesting that many of the rejuvenating interventions act on the stem cell compartments, perhaps reflecting shared genetic and biochemical pathways controlling stem cell function and longevity. Strategy to slow down the aging processes is based on caloric restriction refers to a dietary regimen low in calories but without undernutrition. Sirtuin (SIRT1 and 3, increases longevity by mimicking the beneficial effects of caloric restriction. SIRT3 regulates stress-responsive mitochondrial homeostasis, and more importantly, SIRT3 upregulation rejuvenates aged stem cells in tissues. Resveratrol (3,5,4’-trihydroxystilbene, a natural polyphenol found in grapes and wine, was the most powerful natural activator of SIRT1. In fact, resveratrol treatment has been demonstrated to rescue adult stem cell decline, slow down bodyweight loss, improve trabecular bone structure and mineral density, and significantly extend lifespan. SUMMARY: Tissue-specific stem cells persist throughout the entire lifespan to repair and maintain tissues, but their self-renewal and differentiation potential become dysregulated with aging. Given that adult stem cells are thought to be central to tissue maintenance and organismal

  11. Probing the anti-aging role of polydatin in Caenorhabditis elegans on a chip.

    Science.gov (United States)

    Wen, Hui; Gao, Xinghua; Qin, Jianhua

    2014-01-01

    C. elegans is widely used as a model organism in the study of aging and evaluation of anti-aging drugs due to its unique characteristics. In this work, we set out to investigate polydatin, a natural resveratrol glycoside, and its role in extending lifespan, improving oxidative stress resistance, and the possible regulation mechanism involved in the Insulin/IGF-1 signaling (IIS) pathway for the first time by using a flexible microfluidic device. The effects of polydatin on the lifespan, oxidative stress resistance, mobility and the expression of aging-related proteins and genes were explored. Polydatin was found to significantly extend the mean lifespan of worms by up to 30.7% and 62.1% under normal and acute stress conditions respectively. It improved the expression of the inducible oxidative stress protein (GST-4) and corresponding stroke frequencies in the transgenic CL2166 strain. Moreover, it also increased SOD-3::GFP expression in CF1553 worms and promoted DAF-16 nucleus translocation in TJ356 worms. The longevity-extending role of polydatin is partly attributed to its anti-oxidative activity and increased oxidative stress resistance by regulating the stress-resistance related proteins SOD-3, and daf-16 expression at protein and mRNA levels involved in the IIS pathway. The established microfluidic platform is capable of flexible operation with multiple functions, which not only supports the individual worm's long-term culture with sufficient nutrient exchange, but also facilitates mobility monitoring of the worm, immobilizing and imaging in a controllable and parallel manner. These interesting findings reported here highlight the significance of the natural compound polydatin in the study of aging-related diseases, and the utility of the microfluidic platform for applications in aging studies.

  12. Mitochondrial Protection and Anti-aging Activity of Astragalus Polysaccharides and Their Potential Mechanism

    Directory of Open Access Journals (Sweden)

    Xiao-Juan Xin

    2012-02-01

    Full Text Available The current study was performed to investigate mitochondrial protection and anti-aging activity of Astragalus polysaccharides (APS and the potential underlying mechanism. Lipid peroxidation of liver and brain mitochondria was induced by Fe2+–Vit C in vitro. Thiobarbituric acid (TBA colorimetry was used to measure the content of thiobarbituric acid reactive substances (TBARS. Mouse liver mitochondrial permeability transition (PT was induced by calcium overload in vitro and spectrophotometry was used to measure it. The scavenging activities of APS on superoxide anion (O2•- and hydroxyl radical (•OH, which were produced by reduced nicotinamide adenine dinucleotide (NADH—N-Methylphenazonium methyl sulfate (PMS and hydrogen peroxide (H2O2–Fe2+ system respectively, were measured by 4-nitrobluetetrazolium chloride (NBT reduction and Fenton reaction colorimetry respectively. The Na2S2O3 titration method was used to measure the scavenging activities of APS on H2O2. APS could inhibit TBARS production, protect mitochondria from PT, and scavenge O2•-, •OH and H2O2 significantly in a concentration-dependent manner respectively. The back of the neck of mice was injected subcutaneously with D-galactose to induce aging at a dose of 100 mg/kg/d for seven weeks. Moreover, the activities of catalase (CAT, surperoxide dismutase (SOD and glutathione peroxidase (GPx and anti-hydroxyl radical which were assayed by using commercial monitoring kits were increased significantly in vivo by APS. According to this research, APS protects mitochondria by scavenging reactive oxygen species (ROS, inhibiting mitochondrial PT and increasing the activities of antioxidases. Therefore, APS has the effect of promoting health.

  13. [Anti-aging medicine: notes on a socio-technical controversy].

    Science.gov (United States)

    Leitão, Antônio Nogueira; Pedro, Rosa Maria Leite Ribeiro

    2014-01-01

    After some decades of struggle, geriatrics and gerontology have become the legitimate sciences of aging. Today, their status is being questioned. In its short history, anti-aging medicine has taken root as a medical practice that questions how to address biological aging. In so doing, all medicine is questioned. Here, we explore in particular how this controversy is structured around the founding principles of the sciences of aging. Is there any basis for these questionings? How have they been treated by those who have received them? Taking a socio-technical viewpoint, it is worth considering that for geriatricians and gerontologists, the need to criticize anti-aging medicine also raises some important reflections about how the sciences of aging address their subject.

  14. Purification, characterization, antioxidant activity and anti-aging of exopolysaccharides by Flammulina velutipes SF-06.

    Science.gov (United States)

    Ma, Zhao; Cui, Fangyuan; Gao, Xia; Zhang, Jianjun; Zheng, Lan; Jia, Le

    2015-01-01

    The main objective of this work was to purify the exopolysaccharides (EPS) of Flammulina velutipes SF-06 and investigate the relationship between the different purified fractions and bioactive activity. Two fractions (EPS-1 and EPS-2) were separated and purified by DEAE-52 cellulose and Sephadex G-100 cellulose column chromatography. Monosaccharides composition analysis by gas chromatography indicated that EPS, EPS-1 and EPS-2 were heteropolysaccharides in which rhamnose was a major component. Fourier transform infrared (FT-IR) analysis detected furanose-ring in EPS-1 and EPS-2. All fractions possessed considerable antioxidant activity, while EPS-2 has stronger antioxidant activity than EPS and EPS-1 in vitro. The EPS also exhibited potent anti-aging activation in mice, such as increased catalase and total antioxidant capacity, and decreasing the malondialdehyde (MDA) content. Both the antioxidant in vitro and anti-aging in vivo potentials of EPS could be further utilized in the food industry.

  15. Antioxidant and anti-aging activities of the polysaccharide TLH-3 from Tricholoma lobayense.

    Science.gov (United States)

    Ding, Qiuying; Yang, Dan; Zhang, Wenna; Lu, Yongming; Zhang, Mingzhu; Wang, Liming; Li, Xuehui; Zhou, Liyuan; Wu, Qingxi; Pan, Wenjuan; Chen, Yan

    2016-04-01

    Polysaccharides from edible fungi usually exhibit many bioactivities. Our previous studies found that polysaccharide TLH-3 extracted from Tricholoma lobayense possessed noticeable antioxidant activity. To further explore its biological activities, the antioxidant and anti-aging activities of TLH-3 were evaluated in vitro and in vivo. The results of antioxidant activity in vitro showed that TLH-3 could enhance the cell viability, reduce the production of reactive oxygen species (ROS) and inhibit oxidative damage induced by tert-butylhydroperoxide (t-BHP) in human embryonic lung fibroblasts (HELF) cells. The anti-aging capability was measured in d-galactose (d-gal)-induced aged mice model, and the experimental data showed that TLH-3 significantly inhibited the formation of malondialdehyde (MDA) and raised the activities of superoxide dismutase (SOD) and catalase (CAT) in mice liver and serum (panti-aging activities and could be exploited as a potent dietary supplement to attenuate aging and prevent age-related diseases in humans.

  16. Drosophila melanogaster as a model system for the evaluation of anti-aging compounds.

    Science.gov (United States)

    Jafari, Mahtab

    2010-01-01

    Understanding the causes of aging is a complex problem due to the multiple factors that influence aging, which include genetics, environment, metabolism and reproduction, among others. These multiple factors create logistical difficulties in the evaluation of anti-aging agents. There is a need for good model systems to evaluate potential anti-aging compounds. The model systems used should represent the complexities of aging in humans, so that the findings may be extrapolated to human studies, but they should also present an opportunity to minimize the variables so that the experimental results can be accurately interpreted. In addition to positively affecting lifespan, the impact of the compound on the physiologic confounders of aging, including fecundity and the health span--the period of life where an organism is generally healthy and free from serious or chronic illness--of the model organism needs to be evaluated. Fecundity is considered a major confounder of aging in fruit flies. It is well established that female flies that are exposed to toxic substances typically reduce their dietary intake and their reproductive output and display an artifactual lifespan extension. As a result, drugs that achieve longevity benefits by reducing fecundity as a result of diminished food intake are probably not useful candidates for eventual treatment of aging in humans and should be eliminated during the screening process. Drosophila melanogaster provides a suitable model system for the screening of anti-aging compounds as D. melanogaster and humans have many conserved physiological and biological pathways. In this paper, I propose an algorithm to screen anti-aging compounds using Drosophila melanogaster as a model system.

  17. Phototherapy in anti-aging and its photobiologic basics: a new approach to skin rejuvenation.

    Science.gov (United States)

    Trelles, Mario A

    2006-03-01

    flow, in a noninvasive, athermal manner. New phototherapeutic LED-based systems have appeared to meet the need for a less-expensive but clinically useful light source to enable photoantiaging as a reality in clinical practice. Some studies proving the efficacy of LED therapy have already appeared, and based on their results LED therapy represents a potential new approach to prevention in anti-aging, so that further studies are warranted to prove its efficacy.

  18. Anti-aging effect of simvastatin and telmisartan on retinas and its mechanism in rats%辛伐他汀和替米沙坦对大鼠视网膜衰老的延缓作用及其机制

    Institute of Scientific and Technical Information of China (English)

    王静; 康前雁

    2016-01-01

    Background Statins has prominent roles in regulating lipids,anti-inflammation,autoxidation and protecting vascular endothelial cells.Sartans can promote cell growth and the expression of cytokines.Since the pleiotropic effects of statins and sartans on a variety of cell types,it is inferred that the two medicines can delay retinal aging.Objective This study was to explore the anti-aging effect of simvastatin and telmisartan on the physiological aging of retina.Methods Sixty-six three-month-old healthy SD rats were selected in this study,and 6 of them served as the youth group and the right eyeballs were immediately enucleated.The other rats were raised until 9-month-old in the same conditions and then randomly divided into the simvastatin group,telmisartan group and the control group with 20 rats for each group.The simvastatin of 5 mg/kg and telmisartan of 8 mg/kg were given by intragastric administration once a day in the simvastatin group and the telmisartan group until 17-month-old,and the equal amount of normal saline was used in the control group in the same way.The number of survival rats was 12 in the simvastatin group,10 in the telmisartan group and 8 in the control group.The right eyes were enucleated after heart perfusion of 4% paraformaldehyde solution for the preparation of retinal paraffin sections.Retinal thickness was measured by pathological examination,and the expressions of the retinal neuron markers,including Thy-1,protein kinase C-α (PKC-ot),opsin and rhodopsin,were detected by immunofluorescence technique to evaluate the morphology of retinal ganglion cells (RGCs),bipolar cells as well as the thickness of the outer segment of photoreceptors.Results The retinal structure was clear in the rats of the youth group.However,the RGCs arrangement and inner segment (IS) and outer segment (OS) structure were abnormal in the simvastatin group,the telmisartan group and the control group.Compared with the rats of the youth group,the thickness of outer

  19. Attenuation of replication stress-induced premature cellular senescence to assess anti-aging modalities.

    Science.gov (United States)

    Zhao, Hong; Darzynkiewicz, Zbigniew

    2014-01-01

    Described is an in vitro model of premature senescence in pulmonary adenocarcinoma A549 cells induced by persistent DNA replication stress in response to treatment with the DNA damaging drug mitoxantrone (Mxt). The degree of cellular senescence, based on characteristic changes in cell morphology, is measured by laser scanning cytometry. Specifically, the flattening of cells grown on slides (considered the hallmark of cellular senescence) is measured as the decline in local intensity of DNA-associated DAPI fluorescence (represented by maximal pixels). This change is paralleled by an increase in nuclear area. Thus, the ratio of mean intensity of maximal pixels to nuclear area provides a very sensitive morphometric biomarker for the degree of senescence. This analysis is combined with immunocytochemical detection of senescence markers, such as overexpression of cyclin kinase inhibitors (e.g., p21(WAF1) ) and phosphorylation of ribosomal protein S6 (rpS6), a key marker associated with aging/senescence that is detected using a phospho-specific antibody. These biomarker indices are presented in quantitative terms defined as a senescence index (SI), which is the fraction of the marker in test cultures relative to the same marker in exponentially growing control cultures. This system can be used to evaluate the anti-aging potential of test agents by assessing attenuation of maximal senescence. As an example, the inclusion of berberine, a natural alkaloid with reported anti-aging properties and a long history of use in traditional Chinese medicine, is shown to markedly attenuate the Mxt-induced SI and phosphorylation of rpS6. The multivariate analysis of senescence markers by laser scanning cytometry offers a promising tool to explore the potential anti-aging properties of a variety agents.

  20. The NR4A nuclear receptors as potential targets for anti-aging interventions.

    Science.gov (United States)

    Paillasse, Michael R; de Medina, Philippe

    2015-02-01

    The development of innovative anti-aging strategy is urgently needed to promote healthy aging and overcome the occurrence of age-related diseases such as cancer, diabetes, cardiovascular and neurodegenerative diseases. Genomic instability, deregulated nutrient sensing and mitochondrial dysfunction are established hallmark of aging. Interestingly, the orphan nuclear receptors NR4A subfamily (NR4A1, NR4A2 and NR4A3) are nutrient sensors that trigger mitochondria biogenesis and improve intrinsic mitochondrial function. In addition, NR4A receptors are components of DNA repair machinery and promote DNA repair. Members of the NR4A subfamily should also be involved in anti-aging properties of hormesis since these receptors are induced by various form of cellular stress and stimulate protective cells response such as anti-oxidative activity and DNA repair. Previous studies reported that NR4A nuclear receptors subfamily is potential therapeutic targets for the treatment of age related disorders (e.g. metabolic syndromes, diabetes and neurodegenerative diseases). Consequently, we propose that targeting NR4A receptors might constitute a new approach to delay aging and the onset of diseases affecting our aging population.

  1. Molecular mechanism of extrinsic factors affecting anti-aging of stem cells.

    Science.gov (United States)

    Wong, Tzyy Yue; Solis, Mairim Alexandra; Chen, Ying-Hui; Huang, Lynn Ling-Huei

    2015-03-26

    Scientific evidence suggests that stem cells possess the anti-aging ability to self-renew and maintain differentiation potentials, and quiescent state. The objective of this review is to discuss the micro-environment where stem cells reside in vivo, the secreted factors to which stem cells are exposed, the hypoxic environment, and intracellular factors including genome stability, mitochondria integrity, epigenetic regulators, calorie restrictions, nutrients, and vitamin D. Secreted tumor growth factor-β and fibroblast growth factor-2 are reported to play a role in stem cell quiescence. Extracellular matrices may interact with caveolin-1, the lipid raft on cell membrane to regulate quiescence. N-cadherin, the adhesive protein on niche cells provides support for stem cells. The hypoxic micro-environment turns on hypoxia-inducible factor-1 to prevent mesenchymal stem cells aging through p16 and p21 down-regulation. Mitochondria express glucosephosphate isomerase to undergo glycolysis and prevent cellular aging. Epigenetic regulators such as p300, protein inhibitors of activated Stats and H19 help maintain stem cell quiescence. In addition, calorie restriction may lead to secretion of paracrines cyclic ADP-ribose by intestinal niche cells, which help maintain intestinal stem cells. In conclusion, it is crucial to understand the anti-aging phenomena of stem cells at the molecular level so that the key to solving the aging mystery may be unlocked.

  2. Computer-aided discovery of biological activity spectra for anti-aging and anti-cancer olive oil oleuropeins.

    Science.gov (United States)

    Corominas-Faja, Bruna; Santangelo, Elvira; Cuyàs, Elisabet; Micol, Vicente; Joven, Jorge; Ariza, Xavier; Segura-Carretero, Antonio; García, Jordi; Menendez, Javier A

    2014-09-01

    Aging is associated with common conditions, including cancer, diabetes, cardiovascular disease, and Alzheimer's disease. The type of multi-targeted pharmacological approach necessary to address a complex multifaceted disease such as aging might take advantage of pleiotropic natural polyphenols affecting a wide variety of biological processes. We have recently postulated that the secoiridoids oleuropein aglycone (OA) and decarboxymethyl oleuropein aglycone (DOA), two complex polyphenols present in health-promoting extra virgin olive oil (EVOO), might constitute a new family of plant-produced gerosuppressant agents. This paper describes an analysis of the biological activity spectra (BAS) of OA and DOA using PASS (Prediction of Activity Spectra for Substances) software. PASS can predict thousands of biological activities, as the BAS of a compound is an intrinsic property that is largely dependent on the compound's structure and reflects pharmacological effects, physiological and biochemical mechanisms of action, and specific toxicities. Using Pharmaexpert, a tool that analyzes the PASS-predicted BAS of substances based on thousands of "mechanism-effect" and "effect-mechanism" relationships, we illuminate hypothesis-generating pharmacological effects, mechanisms of action, and targets that might underlie the anti-aging/anti-cancer activities of the gerosuppressant EVOO oleuropeins.

  3. Antioxidant, anti-collagenase and anti-elastase activities of Phyllanthus emblica, Manilkara zapota and silymarin: an in vitro comparative study for anti-aging applications.

    Science.gov (United States)

    Pientaweeratch, Sirinya; Panapisal, Vipaporn; Tansirikongkol, Anyarporn

    2016-09-01

    Context Phyllanthus emblica L. (Euphorbiaceae) (amla), Manilkara zapota L.P. Royen (Sapotaceae) (sapota) and silymarin are reported to contain antioxidant effects. However, information on other biological activities relating to the anti-aging properties is limited. Objective To compare in vitro antioxidants, anti-collagenase (MMP-1 and MMP-2) and anti-elastase properties as well as the phenolic and flavonoid contents of amla, sapota and silymarin as potential anti-aging ingredients. Materials and methods The ethanol amla and sapota fruit extracts were prepared by three cycles of maceration with 24 h duration each. The total phenolic (TPC) and flavonoid (TFC) contents were determined. The antioxidant capacity was evaluated by DPPH and ABTS assays. The effects of MMP-1, MMP-2 and elastase inhibitions were determined by using the EnzChek® assay kits (Molecular-Probes, Eugene, OR). Results Amla exhibited the highest in TPC (362.43 ± 11.2 mg GAE/g) while silymarin showed the highest in TFC (21.04 ± 0.67 mg QE/g). Results of antioxidant activity by DPPH and ABTS methods showed that amla possessed the most potent capacity with IC50 values of 1.70 ± 0.07 and 4.45 ± 0.10 μg/mL, respectively. Highest inhibitions against MMP-1, MMP-2 and elastase were detected for sapota with IC50 values of 89.61 ± 0.96, 86.47 ± 3.04 and 35.73 ± 0.61 μg/mL, respectively. Discussion and conclusion Test extracts offered anti-aging properties in different mechanisms. Amla showed the highest phenolic content and antioxidant property with moderate anti-collagenase. Silymarin exhibited measurable flavonoid content with anti-elastase effect. Sapota showed the highest collagenase and elastase inhibitions with moderate antioxidant effect. Thus, extracts might be added as a mixture to gain the overall anti-aging effects.

  4. Computer-aided discovery of biological activity spectra for anti-aging and anti-cancer olive oil oleuropeins

    Science.gov (United States)

    Corominas-Faja, Bruna; Santangelo, Elvira; Cuyàs, Elisabet; Micol, Vicente; Joven, Jorge; Ariza, Xavier; Segura-Carretero, Antonio; García, Jordi; Menendez, Javier A.

    2014-01-01

    Aging is associated with common conditions, including cancer, diabetes, cardiovascular disease, and Alzheimer's disease. The type of multi-targeted pharmacological approach necessary to address a complex multifaceteddisease such as aging might take advantage of pleiotropic natural polyphenols affecting a wide variety of biological processes. We have recently postulated that the secoiridoids oleuropein aglycone (OA) and decarboxymethyl oleuropein aglycone (DOA), two complex polyphenols present in health-promoting extra virgin olive oil (EVOO), might constitute anew family of plant-produced gerosuppressant agents. This paper describes an analysis of the biological activity spectra (BAS) of OA and DOA using PASS (Prediction of Activity Spectra for Substances) software. PASS can predict thousands of biological activities, as the BAS of a compound is an intrinsic property that is largely dependent on the compound's structure and reflects pharmacological effects, physiological and biochemical mechanisms of action, and specific toxicities. Using Pharmaexpert, a tool that analyzes the PASS-predicted BAS of substances based on thousands of “mechanism-effect” and “effect-mechanism” relationships, we illuminate hypothesis-generating pharmacological effects, mechanisms of action, and targets that might underlie the anti-aging/anti-cancer activities of the gerosuppressant EVOO oleuropeins. PMID:25324469

  5. Mitochondrial decay in ageing: 'Qi-invigorating' schisandrin B as a hormetic agent for mitigating age-related diseases.

    Science.gov (United States)

    Leong, Pou K; Chen, Na; Ko, Kam M

    2012-03-01

    1. The mitochondrial free radical theory of ageing (MFRTA) proposes a primary role for mitochondrial reactive oxygen species (ROS) in the ageing process. The reductive hot spot hypothesis of mammalian ageing serves as a supplement to the MFRTA by explaining how the relatively few cells that have lost oxidative phosphorylation capacity due to mitochondrial DNA mutations can be toxic to the rest of the body and result in the development of age-related diseases. 2. Schisandrin B (SchB), which can induce both a glutathione anti-oxidant and a heat shock response via redox-sensitive signalling pathways, is a hormetic agent potentially useful for increasing the resistance of tissues to oxidative damage. The enhanced cellular/mitochondrial anti-oxidant status and heat shock response afforded by SchB can preserve the structural and functional integrity of mitochondria, suggesting a potential role for SchB in ameliorating age-related diseases. 3. Future studies will focus on investigating whether SchB can produce the hormetic response in humans.

  6. iPSCs-based anti-aging therapies: Recent discoveries and future challenges.

    Science.gov (United States)

    Pareja-Galeano, Helios; Sanchis-Gomar, Fabián; Pérez, Laura M; Emanuele, Enzo; Lucia, Alejandro; Gálvez, Beatriz G; Gallardo, María Esther

    2016-05-01

    The main biological hallmarks of the aging process include stem cell exhaustion and cellular senescence. Consequently, research efforts to treat age-related diseases as well as anti-aging therapies in general have recently focused on potential 'reprogramming' regenerative therapies. These new approaches are based on induced pluripotent stem cells (iPSCs), including potential in vivo reprogramming for tissue repair. Another possibility is targeting pathways of cellular senescence, e.g., through modulation of p16INK4a signaling and especially inhibition of the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB). Here, we reviewed and discussed these recent developments together with their possible usefulness for future treatments against sarcopenia, a major age-related condition.

  7. Pharmacogenomic analysis indicates potential of 1,5-isoquinolinediol as a universal anti-aging agent for different tissues.

    Science.gov (United States)

    Park, Mi Sung; Choi, Joon-Seok; Lee, Wan; Yang, Yoon Jung; Kim, Juhee; Lee, Gun-Joo; Kim, Sang Soo; Park, Seong Hoon; Kim, Sung Chul; Choi, Jin Woo

    2015-07-10

    The natural aging of multicellular organisms is marked by a progressive decline in the function of cells and tissues. The accumulation of senescent cells in tissues seems to eventually cause aging of the host. Nevertheless, gene expression that influences aging is unlikely to be conserved between tissues, and age-related loss of function seems to depend on a variety of mechanisms. This is a concern when developing anti-aging drugs in geriatric clinical pharmacology. We have sought a universal agent to redundantly cover gene expression despite the variation in differentially expressed genes between tissues. Using a minimally modified connectivity map, the poly (ADP-ribose) polymerase (PARP) inhibitor 1,5-isoquinolinediol was selected as a potent candidate, simultaneously applicable to various tissues. This choice was validated in vitro. Treatment of murine embryonic fibroblasts with 1,5-isoquinolinediol appeared to efficiently suppress the rate of replicative senescence at a concentration of 0.1 µM without resulting in cell death. The appearance of abnormal nuclei and accumulation of β-galactosidase in the cytoplasm were inhibited by daily treatment with the agent. When the aging process was accelerated by hydroxyurea-induced oxidative stress, the effect was even more noticeable. Thus, 1,5-isoquinolinediol may potentially be developed as an agent to prolong life.

  8. Bodacious Berry, Potency Wood and the Aging Monster: Gender and Age Relations in Anti-Aging Ads

    Science.gov (United States)

    Calasanti, Toni

    2007-01-01

    This paper situates age discrimination within a broader system of age relations that intersects with other inequalities, and then uses that framework to analyze internet advertisements for the anti-aging industry. Such ads reinforce age and gender relations by positing old people as worthwhile only to the extent that they look and act like those…

  9. Can we delay the accelerated lung aging in COPD? Anti-aging molecules and interventions.

    Science.gov (United States)

    Papaioannou, Andriana I; Rossios, Christos; Kostikas, Konstantinos; Ito, Kazuhiro

    2013-02-01

    Chronic obstructive pulmonary disease (COPD) has been recently characterized as a disease of accelerated lung aging. The prevalence of COPD is age-dependent suggesting an intimate relationship between the pathogenesis of COPD and aging. Lung function decline, the hallmark feature of COPD evolution, is more prominent with increasing age and this decline is greater in smoking individuals. One of the major goals of COPD pharmacotherapy is the development of drugs that would be able to result in a decrease of the decline in lung function over years. However, till nowadays smoking cessation is the only known intervention which is able to decelerate lung function decline. Several mechanisms of aging, including oxidative stress, inflammation and telomere shortening have been shown to be implicated in COPD. Furthermore, numerous anti-aging molecules, including sirtuins and Nrf-2 are reduced, and pathways such as mTOR and genes such as Klotho have also been shown to be abnormal in the lungs of COPD patients. The above mechanisms have been associated with the accelerated lung aging in COPD patients. Numerous therapeutic interventions have been studied in an attempt to reverse accelerated lung aging, and some of them have already been tested in clinical trials. The aim of the present review is to summarize the mechanisms associated with the accelerated lung aging in COPD and to provide information about the possible therapeutic implications targeting those mechanisms.

  10. Systems biology and longevity: an emerging approach to identify innovative anti-aging targets and strategies.

    Science.gov (United States)

    Cevenini, E; Bellavista, E; Tieri, P; Castellani, G; Lescai, F; Francesconi, M; Mishto, M; Santoro, A; Valensin, S; Salvioli, S; Capri, M; Zaikin, A; Monti, D; de Magalhães, J P; Franceschi, C

    2010-01-01

    Human aging and longevity are complex and multi-factorial traits that result from a combination of environmental, genetic, epigenetic and stochastic factors, each contributing to the overall phenotype. The multi-factorial process of aging acts at different levels of complexity, from molecule to cell, from organ to organ systems and finally to organism, giving rise to the dynamic "aging mosaic". At present, an increasing amount of experimental data on genetics, genomics, proteomics and other -omics are available thanks to new high-throughput technologies but a comprehensive model for the study of human aging and longevity is still lacking. Systems biology represents a strategy to integrate and quantify the existing knowledge from different sources into predictive models, to be later tested and then implemented with new experimental data for validation and refinement in a recursive process. The ultimate goal is to compact the new acquired knowledge into a single picture, ideally able to characterize the phenotype at systemic/organism level. In this review we will briefly discuss the aging phenotype in a systems biology perspective, showing four specific examples at different levels of complexity, from a systemic process (inflammation) to a cascade-process pathways (coagulation) and from cellular organelle (proteasome) to single gene-network (PON-1), which could also represent targets for anti-aging strategies.

  11. Advances and challenges in screening traditional Chinese anti-aging materia medica.

    Science.gov (United States)

    Li, Chun-Sheng; Deng, Hong-Bin; Li, Dian-Dong; Li, Zhao-He

    2013-04-01

    To provide a better service for senior health care, we summarized screening studies of traditional Chinese anti-aging materia medica (TCAM). We collected and analyzed literature of TCAM screening studies using the lifespan test and animal models of aging from 1984 to 2012. We found 26 screening methods for TCAM, and 153 single herbs or active ingredients of TCAM that have been screened out during the past 28 years. The cell lifespan test, the fruit fly lifespan test, and D-galactose aging model were the most widely used and intensively studied screening methods. However, the method for establishing the D-galactose aging model needs to be standardized, and the D-galactose aging model cannot completely be a substitute for the normal aging mouse model. Great success has been achieved in screening studies in TCAM. To further improve screening studies in TCAM, we suggest that the D-galactose aging model be incorporated into the lifespan test in the New Drugs of Traditional Chinese Medicine Research Guide.

  12. Consumption of high-dose vitamin C (1250 mg per day) enhances functional and structural properties of serum lipoprotein to improve anti-oxidant, anti-atherosclerotic, and anti-aging effects via regulation of anti-inflammatory microRNA.

    Science.gov (United States)

    Kim, Seong-Min; Lim, So-Mang; Yoo, Jeong-Ah; Woo, Moon-Jea; Cho, Kyung-Hyun

    2015-11-01

    Background Although the health effects of vitamin C are well known, its physiological effect on serum lipoproteins and microRNA still remain to be investigated, especially daily consumption of a high dosage. Objectives To investigate the physiological effect of vitamin C on serum lipoprotein metabolism in terms of its anti-oxidant and anti-glycation activities, and gene expression via microRNA regulation. Methods We analyzed blood parameters and lipoprotein parameters in young subjects (n = 46, 22 ± 2 years old) including smokers who consumed a high dose of vitamin C (1250 mg) daily for 8 weeks. Results Antioxidant activity of serum was enhanced with the elevation of Vit C content in plasma during 8 weeks consumption. In the LDL fraction, the apo-B48 band disappeared at 8 weeks post-consumption in all subjects. In the HDL fraction, apoA-I expression was enhanced by 20% at 8 weeks, especially in male smokers. In the lipoprotein fraction, all subjects showed significantly reduced contents of advanced glycated end products and reactive oxygen species (ROS). Triglyceride (TG) contents in each LDL and HDL fraction were significantly reduced in all groups following the Vit C consumption, suggesting that the lipoprotein was changed to be more anti-inflammatory and atherogenic properties. Phagocytosis of LDL, which was purified from each individual, into macrophages was significantly reduced at 8-weeks post-consumption of vitamin C. Anti-inflammatory and anti-senescence effects of HDL from all subjects were enhanced after the 8-weeks consumption. The expression level of microRNA 155 in HDL3 was reduced by 49% and 75% in non-smokers and smokers, respectively. Conclusion The daily consumption of a high dose of vitamin C for 8 weeks resulted in enhanced anti-senescence and anti-atherosclerotic effects via an improvement of lipoprotein parameters and microRNA expression through anti-oxidation and anti-glycation, especially in smokers.

  13. [Research of anti-aging mechanism of ginsenoside Rg1 on brain].

    Science.gov (United States)

    Li, Cheng-peng; Zhang, Meng-si; Liu, Jun; Geng, Shan; Li, Jing; Zhu, Jia-hong; Zhang, Yan-yan; Jia, Yan-yan; Wang, Lu; Wang, Shun-he; Wang, Ya-ping

    2014-11-01

    Neurodegenerative disease is common and frequently occurs in elderly patients. Previous studies have shown that ginsenoside Rg1 was able to inhibit senescent of brain, but the mechanism on the brain during the treatment remains elucidated. To study the mechanism of ginsenoside Rg1 in the process of anti-aging of brain, forty male SD rats were randomly divided into normal group, Rg1 normal group, brain aging model group and Rg1 brain aging model group, each group with 10 rats (brain aging model group: subcutaneous injection of D-galactose (120 mg kg(-1)), qd for 42 consecutive days; Rg1 brain aging model group: while copying the same test as that of brain aging model group, begin intraperitoneal injection of ginsenosides Rg1 (20 mg x kg(-1)) qd for 27 d from 16 d. Rg1 normal group: subcutaneous injection of the same amount of saline; begin intraperitoneal injection of ginsenosides Rg1 (20 mg x kg(-1)) qd for 27 d from 16 d. Normal: injected with an equal volume of saline within the same time. Perform the related experiment on the second day after finishing copying the model or the completion of the first two days of drug injections). Learning and memory abilities were measured by Morris water maze. The number of senescent cells was detected by SA-beta-Gal staining while the level of IL-1 and IL-6 proinflammatory cytokines in hippocampus were detected by ELISA. The activities of SOD, contents of GSH in hippo- campus were quantified by chromatometry. The change of telomerase activities and telomerase length were performed by TRAP-PCR and southern blotting assay, respectively. It is pointed that, in brain aging model group, the spatial learning and memory capacities were weaken, SA-beta-Gal positive granules increased in section of brain tissue, the activity of antioxidant enzyme SOD and the contents of GSH decreased in hippocampus, the level of IL-1 and IL-6 increased in hippocampus, while the length of telomere and the activity of telomerase decreased in hippocampus

  14. Nonlinear Effects of Nanoparticles: Biological Variability From Hormetic Doses, Small Particle Sizes, and Dynamic Adaptive Interactions

    OpenAIRE

    Bell, Iris R.; Ives, John A.; Wayne B. Jonas

    2013-01-01

    Researchers are increasingly focused on the nanoscale level of organization where biological processes take place in living systems. Nanoparticles (NPs, e.g., 1–100 nm diameter) are small forms of natural or manufactured source material whose properties differ markedly from those of the respective bulk forms of the “same” material. Certain NPs have diagnostic and therapeutic uses; some NPs exhibit low-dose toxicity; other NPs show ability to stimulate low-dose adaptive responses (hormesis). B...

  15. Effect of ZnO nanoparticles on anti-aging properties of polyurethane coating

    Institute of Scientific and Technical Information of China (English)

    WANG ZhenYu; LIU FuChun; HAN EnHou; KE Wei; LUO SuZhen

    2009-01-01

    Polyurethane nano-coatings were prepared by adding nano-concentrates with nanometer zinc oxide (nano-ZnO) to polyurethane coating. The dispersion state of nanoparticles was observed by TEM images. SEM observation and FT-IR analysis indicate that the nano-coating with 1% ZnO nanoparticles can retain better morphological structure than the nano-coating with 5% ZnO nanoparticles after 500 h accelerated aging. It is known from XPS analysis that the anti-oxidation properties of polyurethane coating are enhanced by 1% ZnO nanoparticles through the nano-network and destroyed by 5% ZnO nanoparticles due to the strong light catalysis. A small change in capacitances of nano-coatings with 1% ZnO nanoparticles before and after accelerated aging indicates that 1% ZnO nanoparticles improve the corrosion resistance of coating, while a large increase in capacitances of nano-coating with 5% ZnO nanoparticles before and after accelerated aging demonstrates that 5% ZnO nanoparticles damage the corrosion resistance of coating.

  16. Anti-aging effects of moxa cone moxibustion As a free radical scavenger complement

    Institute of Scientific and Technical Information of China (English)

    Lihua Zhao; Shangjie Chen; Huang Chen; Zhen Qiu

    2011-01-01

    Therapies that complement free radical scavenging are an important approach for treating aging in the brain. In the present study, two formulations of moxa cone moxibustion were applied at acupoints Zusanli (ST 36) and Xuanzhong (GB 39), and at acupoints Baihui (DU 20) and Guanyuan (RN 4), in D-galactose-induced senile mice. The results revealed that moxa cone moxibustion improved total superoxide dismutase and Cu/Zn-superoxide dismutase activity in the homogenates of the cerebral tissue, as well as ameliorating deficits in neuronal morphology and neuronal density in the cerebral cortex and hippocampal CA3. Moxa cone moxibustion also enhanced learning and memory functions of senile mice. Moxa cone moxibustion at Zusanli, Xuanzhong, Baihui and Guanyuan acupoints can thus be used to complement free radical scavengers, with efficacy that is equal to that of electroacupuncture at Zusanli and Xuanzhong, and superior to that of nimodipine treatment.

  17. 白藜芦醇在抗衰老领域的研究进展%Recent progress of resveratrol in anti-aging field

    Institute of Scientific and Technical Information of China (English)

    李博; 宁光; 张志国

    2013-01-01

    Resveratrol is a polyphenol compound originally extracted from giant knotweed,grape,and red wine.Previous research demonstrated calorie restriction has anti-aging effect.Recent studies showed that resveratrol could mimick calorie restriction,thereby play an positive role in anti-aging field.Moreover,it has anti-diabetic,cardioprotective and neuroprotective effects as well.The potential targets and the underlying mechanisms of resveratrol have begun to be elucidated.Meanwhile,accumulating data of its effectiveness and safety from clinical studies also contribute to pave the way for its further clinical application.%白藜芦醇是一种存在于中药虎杖、葡萄以及红酒中的多酚类化合物.既往研究表明,热量限制具有抗衰老的作用.近年研究发现,白藜芦醇能模拟热量限制,在抗衰老领域发挥积极的作用,并且具有抗糖尿病、保护心血管和神经功能的作用;白藜芦醇的生物学靶点和作用机制也开始被了解.同时,白藜芦醇临床试验所获得的疗效及安全性研究结果为其将来临床应用打下了基础.

  18. Incorporation of Copper Enhances the Anti-Ageing Property of Flame-Sprayed High-Density Polyethylene Coatings

    Science.gov (United States)

    Jia, Zhengmei; Huang, Jing; Gong, Yongfeng; Jin, Peipeng; Suo, Xinkun; Li, Hua

    2017-02-01

    High-density polyethylene (HDPE)-copper (Cu) composite coatings were prepared through depositing HDPE-Cu core-shell particles by flame spraying. The HDPE-Cu composite coatings and the HDPE coatings were aged in xenon lamp ageing testing chamber. The variations of chemical compositions and surface morphology of the coatings before and after the ageing testing were analyzed using infrared spectroscopy, scanning electron microscopy, thermogravimetric analysis, differential scanning calorimetry and ultraviolet-visible spectrophotometer. Results show that there is no chemical composition variation in the HDPE-Cu coatings. Cracks were found on the surfaces of the HDPE coatings, while the HDPE-Cu coating shows almost intact surface morphology. These results suggest that the HDPE-Cu coatings present better anti-ageing performances than the HDPE coatings. Further assessment of the function of Cu shells on the anti-ageing property reveals that Cu shells not only enhanced the absorption of the coatings to ultraviolet, but also increased their reflectivity to visible light. Additionally, the Cu shells enhanced the decomposition temperature and thermal stability of HDPE in the composite coatings. These results give bright insight into potential anti-ageing applications of the polymer-based structures.

  19. New fashioned anti-aging cosmetic market%引领潮流的抗衰老化妆品市场

    Institute of Scientific and Technical Information of China (English)

    任静

    2012-01-01

    对亚洲及中国的抗衰老化妆品市场进行了论述,并对我国的抗衰老化妆品市场增长的因素进行了分析。介绍了几种抗衰老产品中的新原料以及几种新的抗衰老化妆品品牌。%The anti-aging cosmetic market in China and Asia were related, and the increasing factor of anti-aging cosmetic market in China was analyzed. Some new raw materials used in anti-aging cosmetics were introduced, and the anti-aging cosmetic brarLds were also included.

  20. Efficacy and Tolerability of a Skin Brightening/Anti-Aging Cosmeceutical Containing Retinol 0.5%, Niacinamide, Hexylresorcinol, and Resveratrol.

    Science.gov (United States)

    Farris, Patricia; Zeichner, Joshua; Berson, Diane

    2016-07-01

    Consumers are increasingly interested in over-the-counter skin care products that can improve the appearance of photodamaged and aging skin. This 10-week, open-label, single- center study enrolled 25 subjects with mild to moderate hyperpigmentation and other clinical stigmata of cutaneous aging including fine lines, sallowness, lack of clarity, and wrinkling. Their mean age was 53.4±7.7 years. The test product contained retinol 0.5% in combination with niacinamide 4.4%, resveratrol 1%, and hexylresorcinol 1.1% in a moisturizing base. Subjects were provided a skin care regimen including a cleanser, hydrating serum, moisturizer, and an SPF 30 sunscreen for daily use. The test product was applied only at night. The use of this skin brightening/anti-aging cosmeceutical was found to provide statistically significant improvements in all efficacy endpoints by study end. Fine lines, radiance, and smoothness were significantly improved as early as week 2 (P The results of this open-label clinical study suggest that a topical cream containing retinol 0.5% in combination with niacinamide, resveratrol, and hexylresorcinol is efficacious and tolerable for skin brightening/anti-aging when used with a complementary skin care regimen including SPF 30 sun protection. J Drugs Dermatol. 2016;15(7):863-868.

  1. Xenohormetic and anti-aging activity of secoiridoid polyphenols present in extra virgin olive oil: a new family of gerosuppressant agents.

    Science.gov (United States)

    Menendez, Javier A; Joven, Jorge; Aragonès, Gerard; Barrajón-Catalán, Enrique; Beltrán-Debón, Raúl; Borrás-Linares, Isabel; Camps, Jordi; Corominas-Faja, Bruna; Cufí, Sílvia; Fernández-Arroyo, Salvador; Garcia-Heredia, Anabel; Hernández-Aguilera, Anna; Herranz-López, María; Jiménez-Sánchez, Cecilia; López-Bonet, Eugeni; Lozano-Sánchez, Jesús; Luciano-Mateo, Fedra; Martin-Castillo, Begoña; Martin-Paredero, Vicente; Pérez-Sánchez, Almudena; Oliveras-Ferraros, Cristina; Riera-Borrull, Marta; Rodríguez-Gallego, Esther; Quirantes-Piné, Rosa; Rull, Anna; Tomás-Menor, Laura; Vazquez-Martin, Alejandro; Alonso-Villaverde, Carlos; Micol, Vicente; Segura-Carretero, Antonio

    2013-02-15

    Aging can be viewed as a quasi-programmed phenomenon driven by the overactivation of the nutrient-sensing mTOR gerogene. mTOR-driven aging can be triggered or accelerated by a decline or loss of responsiveness to activation of the energy-sensing protein AMPK, a critical gerosuppressor of mTOR. The occurrence of age-related diseases, therefore, reflects the synergistic interaction between our evolutionary path to sedentarism, which chronically increases a number of mTOR activating gero-promoters (e.g., food, growth factors, cytokines and insulin) and the "defective design" of central metabolic integrators such as mTOR and AMPK. Our laboratories at the Bioactive Food Component Platform in Spain have initiated a systematic approach to molecularly elucidate and clinically explore whether the "xenohormesis hypothesis," which states that stress-induced synthesis of plant polyphenols and many other phytochemicals provides an environmental chemical signature that upregulates stress-resistance pathways in plant consumers, can be explained in terms of the reactivity of the AMPK/mTOR-axis to so-called xenohormetins. Here, we explore the AMPK/mTOR-xenohormetic nature of complex polyphenols naturally present in extra virgin olive oil (EVOO), a pivotal component of the Mediterranean style diet that has been repeatedly associated with a reduction in age-related morbid conditions and longer life expectancy. Using crude EVOO phenolic extracts highly enriched in the secoiridoids oleuropein aglycon and decarboxymethyl oleuropein aglycon, we show for the first time that (1) the anticancer activity of EVOO secoiridoids is related to the activation of anti-aging/cellular stress-like gene signatures, including endoplasmic reticulum (ER) stress and the unfolded protein response, spermidine and polyamine metabolism, sirtuin-1 (SIRT1) and NRF2 signaling; (2) EVOO secoiridoids activate AMPK and suppress crucial genes involved in the Warburg effect and the self-renewal capacity of "immortal

  2. Combined anti-ages and antioxidant activities of different solvent extracts of Solanum elaeagnifolium Cav (Solanacea) fruits during ripening and related to their phytochemical compositions

    Science.gov (United States)

    Houda, Mejri; Derbré, Séverine; Jedy, Ahmed; Tlili, Nizar; Legault, Jean; Richomme, Pascal; Limam, Ferid; Saidani-Tounsi, Moufida

    2014-01-01

    Oxidative stress and advanced glycation end products (AGEs) are known as key factors for the development of diabetic complications such as retinopathy, cataract as well as atherosclerosis and neurodegenerative diseases, including Alzheimer’s diseases. In this context, natural products have been previously identified as promising sources for antioxidant and anti-glycation compounds. The current study focuses on the evaluation of antioxidant and glycation inhibitory activities of different solvent extracts of Solanum elaeagnifolium Cav (Solanaceae) fruits at different ripening stages. The results showed that antioxidant and anti-AGEs activities were significantly influenced by solvents polarities and ripening stages of S. elaeagnifolium Cav. With one exception, methanolic extract of overripe S. elaeagnifolium Cav fruit showed important protective effects against cellular oxidative stress. The aqueous extract showed the highest ABTS+ scavenging ability. Principal component analysis showed that total phenolic and flavonoid contents correlated well with observed antioxidants and anti-glycation activities. These results bring attention to the possible use of S. elaeagnifolium Cav as a valuable source of bioactive compounds exhibiting antioxidant effects and potentially alleviating diabetic complications. PMID:26417319

  3. 日益活跃的抗衰老系列产品%Getting active in anti-aging

    Institute of Scientific and Technical Information of China (English)

    刘晓英

    2012-01-01

    With the growth of aging population in the world,consumer needs can't be met by skin care products.Among the numerous active ingredients,few of them can win the trusts of consumers for a long period.Only these manufacturers in anti-aging taking steps in the right direction can obtain better development.%随着世界人口老龄化的加剧,护肤产品已经不能完全满足许多消费者的消费需求。在众多的活性成分中,鲜有能够赢得消费者的长期信任。只有那些向正确的方向迈进的抗衰老产品生产企业,才能够获得更好的发展。

  4. Metformin and cancer: doses, mechanisms and the dandelion and hormetic phenomena.

    Science.gov (United States)

    Martin-Castillo, Begoña; Vazquez-Martin, Alejandro; Oliveras-Ferraros, Cristina; Menendez, Javier A

    2010-03-15

    In the early 1970s, Professor Vladimir Dilman originally developed the idea that antidiabetic biguanides may be promising as geroprotectors and anticancer drugs ("metabolic rehabilitation").  In the early 2000s, Anisimov´s experiments revealed that chronic treatment of female transgenic HER2-/neu mice with metformin significantly reduced the incidence and size of mammary adenocarcinomas and increased the mean latency of the tumors.  Epidemiological studies have confirmed that metformin, but not other anti-diabetic drugs, significantly reduces cancer incidence and improves cancer patients' survival in type 2 diabetics.  At present, pioneer work by Dilman & Anisimov at the Petrov Institute of Oncology (St. Petersburg, Russia) is rapidly evolving due to ever-growing preclinical studies using human tumor-derived cultured cancer cells and animal models. We herein critically review how the antidiabetic drug metformin is getting reset to metabolically fight cancer. Our current perception is that metformin may constitute a novel "hybrid anti-cancer pill" physically combining both the long-lasting effects of antibodies -by persistently lowering levels of blood insulin and glucose- and the immediate potency of a cancer cell-targeting molecular agent -by suppressing the pivotal AMPK/mTOR/S6K1 axis and several protein kinases at once, including tyrosine kinase receptors such as HER1 and HER2-.  In this scenario, we discuss the relevance of metformin doses in pre-clinical models regarding metformin's mechanisms of action in clinical settings. We examine recent landmark studies demonstrating metformin's ability to specifically target the cancer-initiating stem cells from which tumor cells develop, thereby preventing cancer relapse when used in combination with cytotoxic chemotherapy (dandelion hypothesis).  We present the notion that, by acting as an efficient caloric restriction mimetic, metformin enhanced intrinsic capacity of mitotically competent cells to self

  5. Investigating a Potential Auxin-Related Mode of Hormetic/Inhibitory Action of the Phytotoxin Parthenin.

    Science.gov (United States)

    Belz, Regina G

    2016-01-01

    Parthenin is a metabolite of Parthenium hysterophorus and is believed to contribute to the weed's invasiveness via allelopathy. Despite the potential of parthenin to suppress competitors, low doses stimulate plant growth. This biphasic action was hypothesized to be auxin-like and, therefore, an auxin-related mode of parthenin action was investigated using two approaches: joint action experiments with Lactuca sativa, and dose-response experiments with auxin/antiauxin-resistant Arabidopsis thaliana genotypes. The joint action approach comprised binary mixtures of subinhibitory doses of the auxin 3-indoleacetic acid (IAA) mixed with parthenin or one of three reference compounds [indole-3-butyric acid (IBA), 2,3,5-triiodobenzoic acid (TIBA), 2-(p-chlorophenoxy)-2-methylpropionic acid (PCIB)]. The reference compounds significantly interacted with IAA at all doses, but parthenin interacted only at low doses indicating that parthenin hormesis may be auxin-related, in contrast to its inhibitory action. The genetic approach investigated the response of four auxin/antiauxin-resistant mutants and a wildtype to parthenin or two reference compounds (IAA, PCIB). The responses of mutant plants to the reference compounds confirmed previous reports, but differed from the responses observed for parthenin. Parthenin stimulated and inhibited all mutants independent of resistance. This provided no indication for an auxin-related action of parthenin. Therefore, the hypothesis of an auxin-related inhibitory action of parthenin was rejected in two independent experimental approaches, while the hypothesis of an auxin-related stimulatory effect could not be rejected.

  6. Hormesis-based anti-aging products: a case study of a novel cosmetic

    DEFF Research Database (Denmark)

    Rattan, Suresh; Kryzch, Valerie; Schnebert, Sylvianne

    2013-01-01

    and cosmeceuticals. Here we present the example of a skin care cosmetic as one of the first successful product developments incorporating the ideas of hormesis. This was based on the studies to analyse the molecular effects of active ingredients extracted from the roots of the Chinese herb Sanchi (Panax notoginseng......, nutritional and mental hormetins...

  7. Design Space Approach for Preservative System Optimization of an Anti-Aging Eye Fluid Emulsion.

    Science.gov (United States)

    Lourenço, Felipe Rebello; Francisco, Fabiane Lacerda; Ferreira, Márcia Regina Spuri; Andreoli, Terezinha De Jesus; Löbenberg, Raimar; Bou-Chacra, Nádia

    2015-01-01

    The use of preservatives must be optimized in order to ensure the efficacy of an antimicrobial system as well as the product safety. Despite the wide variety of preservatives, the synergistic or antagonistic effects of their combinations are not well established and it is still an issue in the development of pharmaceutical and cosmetic products. The purpose of this paper was to establish a space design using a simplex-centroid approach to achieve the lowest effective concentration of 3 preservatives (methylparaben, propylparaben, and imidazolidinyl urea) and EDTA for an emulsion cosmetic product. Twenty-two formulae of emulsion differing only by imidazolidinyl urea (A: 0.00 to 0.30% w/w), methylparaben (B: 0.00 to 0.20% w/w), propylparaben (C: 0.00 to 0.10% w/w) and EDTA (D: 0.00 to 0.10% w/w) concentrations were prepared. They were tested alone and in binary, ternary and quaternary combinations. Aliquots of these formulae were inoculated with several microorganisms. An electrochemical method was used to determine microbial burden immediately after inoculation and after 2, 4, 8, 12, 24, 48, and 168 h. An optimization strategy was used to obtain the concentrations of preservatives and EDTA resulting in a most effective preservative system of all microorganisms simultaneously. The use of preservatives and EDTA in combination has the advantage of exhibiting a potential synergistic effect against a wider spectrum of microorganisms. Based on graphic and optimization strategies, we proposed a new formula containing a quaternary combination (A: 55%; B: 30%; C: 5% and D: 10% w/w), which complies with the specification of a conventional challenge test. A design space approach was successfully employed in the optimization of concentrations of preservatives and EDTA in an emulsion cosmetic product.

  8. Development on Anti-ageing of Unsaturated Polyester Glass Fiber Reinforced Plastic (GFRP) Composites%以不饱和聚酯树脂为基的玻璃钢复合材料防老化研究进展

    Institute of Scientific and Technical Information of China (English)

    王玉民; 郭振宇; 宁培森; 丁著明

    2011-01-01

    综述了不饱和聚酯树脂基玻璃钢复合材料(GFRP)防老化方面的最近研究进展,包括GFRP表面新型涂层及树脂的添加剂(紫外线吸收剂、受阻胺光稳定剂和抗氧剂等).结果表明,只使用单一的稳定剂效果不佳,必须将抗氧剂和其他添加剂(例如某些环氧化合物)并用,才能取得较好的效果.%Recent advances in anti-ageing of unsatured polyester glass fiber reinforced plastic (GFRP) were reviewed in this paper, including novel coating of GFRP and adding various stabilizers, such as ultraviolet absorbent,hindered amine light stabilizers(HALS) and antioxidant. The results showed the effect was not good using single stabilizer. The combined use of Main and side antioxidant and other additives (e. g. some epoxy compounds) had the best Effect on maintaining anti-ageing of GFRP

  9. Evaluation of skin anti-aging potential of Citrus reticulata blanco peel

    Directory of Open Access Journals (Sweden)

    Vinita D Apraj

    2016-01-01

    Abbreviation Used: ECM: Extracellular matrix, UV: Ultra violet, ROS: Reactive Oxygen Species, MMP: Matrix metalloproteinase, Chc: Clostridium histolyticum collagenase, DPPH: 2, 2-diphenyl-1-picrylhydrazyl, GC-MS: Gas Chromatography- Mass Spectroscopy, RT: Room Temperature, μg GAE/ mg: Microgram Gallic acid equivalent / milligram, W/V: Weight by Volume, μg QE/ mg: Microgram Quercetin equivalent / milligram, CR HAE: Hot Alcoholic Extract of Citrus reticulata Blanco, CR CAE: Cold Alcoholic Extract of Citrus reticulata Blanco, EC50: Half Maximal Effective Concentration, PMS NADH: Phenazine methosulfate nicotinamide adenine dinucleotide, NBT: Nitroblue tetrazolium, DMSO: Dimethyl sulfoxide, APS: Ammonium Persulphate, AAPH: 2,2 -azobis(2-amidino-propane dihydrochloride, TROLOX: (± 6-hydroxy-2,5,7,8-tetramethyl chromane-2-carboxylic acid, ORAC: Oxygen Radical Absorbance Capacity, FALGPA: N-[3-(2-Furyl acryloyl]-Leu-Gly-Pro-Ala, SANA: Succinyl-Ala-Ala-Ala-p-nitroanilide, Rf: Retardation Factor, MSD: Mass Selective Detector

  10. Lifestyle and nutrition, caloric restriction, mitochondrial health and hormones: Scientific interventions for anti-aging

    Directory of Open Access Journals (Sweden)

    Luis Vitetta

    2008-01-01

    Full Text Available Luis Vitetta1, Bill Anton21Unit of Health Integration, School of Medicine, University of Queensland, Australia; 2Path Lab, Melbourne, AustraliaAbstract: Aging is a universal process to all life forms. The most current and widely accepted definition for aging in humans is that there is a progressive loss of function and energy production that is accompanied by decreasing fertility and increasing mortality with advancing age. The most obvious and commonly recognised consequence of aging and energy decline is a decrease in skeletal muscle function which affects every aspect of human life from the ability to play games, walk and run to chew, swallow and digest food. There is hence a recognised overall decline of an individuals’ fitness for the environment that they occupy. In Westernised countries this decline is gradual and the signs become mostly noticeable after the 5th decade of life and henceforth, where the individual slowly progresses to death over the next three to four decades. Given that the aging process is slow and gradual, it presents with opportunities and options that may ameliorate and improve the overall functional capacity of the organism. Small changes in function may be more amenable and likely to further slow down and possibly reverse some of the deleterious effects of aging, rather, than when the incremental changes are large. This overall effect may then translate into a significant compression of the deleterious aspects of human aging with a resultant increase in human life expectancy.Keywords: human aging, life expectancy, lifespan, energy

  11. A Novel Cassia fistula (L.-Based Emulsion Elicits Skin Anti-Aging Benefits in Humans

    Directory of Open Access Journals (Sweden)

    Barkat Ali Khan

    2015-11-01

    Full Text Available Cassia fistula, a flowering plant in the family of Caesalpinaceae (Fabaceae, is used in traditional medicine for several indications. Nevertheless, too little is known about its effects on skin conditions and skin aging. Therefore, in this pioneering study, the extracts of oil-in-water macro-emulsions containing 5% C. fistula (L. crude pods (i.e., phyto-active formulation were optimally developed and compared to the placebo (i.e., emulsions without the crude extract for assessment of their effects on human skin aging. Healthy adult male volunteers (n = 13 with a mean age of 31 ± 5.5 years (range: 24–47 years were enrolled after informed written consent. For 12 consecutive weeks, the subjects were directed to use a patch containing the active emulsion on one of their forearms as well as a patch containing the placebo on their other forearm. Biometrological measurements of skin hydration (SH and transepidermal water loss (TEWL were performed on both sides of their respective cheeks at time 0 (baseline values, 2, 4, 6, 8, 10 and 12th weeks. Surface evaluation of living skin (SELS was taken at time 0 (baseline values or after 1, 2 and 3 months. Topical application of C. fistula extracts showed a significant (p < 0.05 increase in stratum corneum hydration level, a significant enhancement in its water-holding function as well as in its barrier function. Further, significant (p < 0.005 ameliorations of skin aspects were observed (i.e., less roughness, less dryness, less wrinkles. Taken together, our results strongly suggest therapeutic and esthetic potential of C. fistula pod’s extracts to prevent or delay human skin aging.

  12. One size may not fit all: anti-aging therapies and sarcopenia.

    Science.gov (United States)

    Burks, Tyesha N; Cohn, Ronald D

    2011-12-01

    Sarcopenia refers to age-related loss of muscle mass and function. Several age-related changes occur in skeletal muscle including a decrease in myofiber size and number and a diminished ability of satellite cells to activate and proliferate upon injury leading to impaired muscle remodeling. Although the molecular mechanisms underlying sarcopenia are unknown, it is tempting to hypothesize that interplay between biological and environmental factors cooperate in a positive feedback cycle contributing to the progression of sarcopenia. Indeed many essential biological mechanisms such as apoptosis and autophagy and critical signaling pathways involved in skeletal muscle homeostasis are altered during aging and have been linked to loss of muscle mass. Moreover, the environmental effects of the sedentary lifestyle of older people further promote and contribute the loss of muscle mass. There are currently no widely accepted therapeutic strategies to halt or reverse the progression of sarcopenia. Caloric restriction has been shown to be beneficial as a sarcopenia and aging antagonist. Such results have made the search for caloric restriction mimetics (CRM) a priority. However given the mechanisms of action, some of the currently investigated CRMs may not combat sarcopenia. Thus, sarcopenia may represent a unique phenotypic feature of aging that requires specific and individually tailored therapeutic strategies.

  13. [From the right to health to the responsibility for healthy aging: a new foundation for anti-aging medicine in Germany].

    Science.gov (United States)

    Spindler, M

    2010-03-01

    This paper explores the problem of dealing with normalisation in public health concepts, using the example of the relaunching of anti-aging-medicine in Germany. The analysis mainly draws upon qualitative analyses of publications of different anti-aging-medicine organisations. The German branch of anti-aging-medicine's new concepts of the nature, the morality and the self-design of aging are delineated. These are the concepts of aging as a medically controllable health risk (nature), the emphasis of the moral duty to responsible personal risk control (morality) and the self-design through individual risk profiles and prevention programmes. The analysis shows that the proposed bio-political programme cannot only be induced from biologically, societally or medically given facts, but that it is - as all public health concepts - a normative concept. It is argued that the normative aspects of public health concepts cannot be avoided but should be explicated and negotiated. This paper therefore concludes with an exploration of what there is to negotiate concerning the German anti-aging-physicians' surprisingly uncontroversial plea for a shift of emphasis from the collective right to health to a new individual duty for health maintenance in old age.

  14. 小鼠抗衰老实验及微生物抗衰老研究概况%Anti-aging research with mice and microbes

    Institute of Scientific and Technical Information of China (English)

    陈君; 杨光; 刘文群

    2011-01-01

    Aging is a multidimensional process of the changes in an organism over time, leading to decline in function and disorders. The research on anti-aging is an important issue of life science due to the pursuit of human health and longevity. The relationship between antioxidant and anti-aging,the mice model of aging induced by D-galactose, and the physiological indexes for anti-aging experiments were introduced, as well as the current research on anti-aging and anti-oxidative problems of microbes.%衰老是机体在退化时期功能下降和紊乱的综合表现,抗衰老问题是人类为追求健康长寿进行生命科学研究的重要课题.文中主要介绍了抗氧化与抗衰老的关系、采用D-半乳糖所致小鼠衰老模型、关于抗衰老实验的生理指标内容以及微生物抗氧化抗衰老研究概况.

  15. 枸杞多糖的提取及其抗衰老的研究%Research on the Extraction and Anti-aging of Lycium Barbarum Polysaccharide

    Institute of Scientific and Technical Information of China (English)

    刘杰; 潘晓秋; 周晖; 张海林; 齐宏亮; 廖丹; 倪雪松

    2016-01-01

    目的:优选枸杞多糖的最佳提取工艺,通过建立D-半乳糖致衰老小鼠模型,研究枸杞多糖的抗衰老作用。方法:采用正交设计法对提取工艺进行研究;应用D-半乳糖连续注射,造成实验性衰老小鼠模型,通过测定血液丙二醛(MDA)、谷胱甘肽过氧化物酶( GSH-PX)和过氧化氢酶( CAT)的含量及超氧化物歧化酶( SOD)的活力和皮肤中SOD、MDA及羟脯氨酸( Hyp)的水平来检测枸杞多糖抗衰老的能力。结果:通过正交设计确定的枸杞多糖的最佳提取方案为:料液比为1∶30,提取2次,1次90 min,功率为100 kHz。通过研究可见,枸杞多糖可提高小鼠血液中SOD、CAT、GSH-px水平,降低MDA值;可提高小鼠皮肤SOD活力,降低皮肤MDA含量,提高Hyp含量。结论:本研究建立的提取方法简单、易行,枸杞多糖的提出率高。枸杞多糖具有抗D-半乳糖所致小鼠衰老的作用。%OBJECTIVE:To optimize the optimum extraction technology of lycium barbarum polysaccharide and establish senile mice model by D-galactose , so as to research the anti-aging effects of lycium barbarum polysaccharide . METHODS:Orthogonal design method was adopted to research the extraction technology .The senile mice model was established by injection of D-galactose .The anti-aging capacibility of lycium barbarum polysaccharide were determined by the detection of malonaldehyde (MDA), glutathione peroxidase (GSH-PX), the content of catalase (CAT), activity of superoxide dismutase (SOD) and level of SOD, MDA and hydroxyproline (Hyp) in the skin.RESULTS:The optimum extraction technology scheme by orthogonal design method were : the ratio of solid to liquid was 1∶30, 2 times of extraction, 90 min for once, the power was 100 kHz.Lycium barbarum polysaccharide could increase the level of SOD , CAT and GSH-px in rats , decrease MDA , improve the SOD activity , decrease MDA and increase the content of Hyp

  16. ASM-3 acid sphingomyelinase functions as a positive regulator of the DAF-2/AGE-1 signaling pathway and serves as a novel anti-aging target.

    Science.gov (United States)

    Kim, Yongsoon; Sun, Hong

    2012-01-01

    In C. elegans, the highly conserved DAF-2/insulin/insulin-like growth factor 1 receptor signaling (IIS) pathway regulates longevity, metabolism, reproduction and development. In mammals, acid sphingomyelinase (ASM) is an enzyme that hydrolyzes sphingomyelin to produce ceramide. ASM has been implicated in CD95 death receptor signaling under certain stress conditions. However, the involvement of ASM in growth factor receptor signaling under physiological conditions is not known. Here, we report that in vivo ASM functions as a positive regulator of the DAF-2/IIS pathway in C. elegans. We have shown that inactivation of asm-3 extends animal lifespan and promotes dauer arrest, an alternative developmental process. A significant cooperative effect on lifespan is observed between asm-3 deficiency and loss-of-function alleles of the age-1/PI 3-kinase, with the asm-3; age-1 double mutant animals having a mean lifespan 259% greater than that of the wild-type animals. The lifespan extension phenotypes caused by the loss of asm-3 are dependent on the functions of daf-16/FOXO and daf-18/PTEN. We have demonstrated that inactivation of asm-3 causes nuclear translocation of DAF-16::GFP protein, up-regulates endogenous DAF-16 protein levels and activates the downstream targeting genes of DAF-16. Together, our findings reveal a novel role of asm-3 in regulation of lifespan and diapause by modulating IIS pathway. Importantly, we have found that two drugs known to inhibit mammalian ASM activities, desipramine and clomipramine, markedly extend the lifespan of wild-type animals, in a manner similar to that achieved by genetic inactivation of the asm genes. Our studies illustrate a novel strategy of anti-aging by targeting ASM, which may potentially be extended to mammals.

  17. Resveratrol increases anti-aging Klotho gene expression via the activating transcription factor 3/c-Jun complex-mediated signaling pathway.

    Science.gov (United States)

    Hsu, Shih-Che; Huang, Shih-Ming; Chen, Ann; Sun, Chiao-Yin; Lin, Shih-Hua; Chen, Jin-Shuen; Liu, Shu-Ting; Hsu, Yu-Juei

    2014-08-01

    The Klotho gene functions as an aging suppressor gene. Evidence from animal models suggests that induction of Klotho expression may be a potential treatment for age-associated diseases. However, the molecular mechanism involved in regulating renal Klotho gene expression remains unclear. In this study, we determined that resveratrol, a natural polyphenol, induced renal Klotho expression both in vivo and in vitro. In the mouse kidney, resveratrol administration markedly increased both Klotho mRNA and protein expression. In resveratrol-treated NRK-52E cells, increased Klotho expression was accompanied by the upregulation and nuclear translocation of activating transcription factor 3 (ATF3) and c-Jun. ATF3 or c-Jun overexpression enhanced the transcriptional activation of Klotho. Conversely, resveratrol-induced Klotho expression was attenuated in the presence of dominant-negative ATF3 or c-Jun. Coimmunoprecipitation and a chromatin immunoprecipitation assay revealed that ATF3 physically interacted with c-Jun and that the ATF3/c-Jun complex directly bound to the Klotho promoter through ATF3- and AP-1-binding elements. c-Jun cotransfection augmented the effects of ATF3 on Klotho transcription in vitro. Although Sirtuin 1 mRNA expression was induced by resveratrol and involved in regulating Klotho mRNA expression, it was not the primary cause for the aforementioned ATF3/c-Jun pathway. In summary, resveratrol enhances the renal expression of the anti-aging Klotho gene, and the transcriptional factors ATF3 and c-Jun functionally interact and coordinately regulate the resveratrol-mediated transcriptional activation of Klotho.

  18. Ayurvedic Amalaki Rasayana promotes improved stress tolerance and thus has anti-aging effects in Drosophila melanogaster.

    Science.gov (United States)

    Dwivedi, Vibha; Lakhotia, Subhash C

    2016-12-01

    Amalaki Rasayana (AR) is a common Ayurvedic herbal formulation of Phyllanthus emblica fruits and some other ingredients, and is used for general good health and healthy aging. We reported it to improve life history traits and to suppress neurodegeneration as well as induced apoptosis in Drosophila. The present study examines responses of Drosophila reared on AR-supplemented food to crowding, thermal or oxidative stresses. Wild-type larvae/flies reared on AR-supplemented food survived the various cell stresses much better than those reared on control food. AR-fed mutant park13 or DJ-1 beta Delta93 (Parkinson's disease model) larvae/flies, however, showed only partial or no protection, respectively, against paraquat-induced oxidative stress, indicating essentiality of DJ-1 beta for AR-mediated oxidative stress tolerance. AR feeding reduced the accumulation of reactive oxygen species (ROS) and lipid peroxidation even in aged (35-day-old) wild-type flies while enhancing superoxide dismutase (SOD) activity. We show that while Hsp70 or Hsp83 expression under normal or stress conditions was not affected by AR feeding, Hsp27 levels were elevated in AR-fed wild-type control as well as heat-shocked larvae. Therefore, besides the known anti-oxidant activity of Phyllanthus emblica fruits, dietary AR also enhances cellular levels of Hsp27. Our in vivo study on a model organism shows that AR feeding significantly improves tolerance to a variety of cell stresses through reduced ROS and lipid peroxidation on the one hand, and enhanced SOD activity and Hsp27 on the other. The resulting better homeostasis improves life span and quality of organism's life.

  19. Ayurvedic Amalaki Rasayana promotes improved stress tolerance and thus has anti-aging effects in Drosophila melanogaster

    Indian Academy of Sciences (India)

    VIBHA DWIVEDI; SUBHASH C LAKHOTIA

    2016-12-01

    Amalaki Rasayana (AR) is a common Ayurvedic herbal formulation of Phyllanthus emblica fruits and some otheringredients, and is used for general good health and healthy aging. We reported it to improve life history traits and tosuppress neurodegeneration as well as induced apoptosis in Drosophila. The present study examines responses ofDrosophila reared on AR-supplemented food to crowding, thermal or oxidative stresses. Wild-type larvae/flies rearedon AR-supplemented food survived the various cell stresses much better than those reared on control food. AR-fedmutant park13 or DJ-1βDelta93 (Parkinson’s disease model) larvae/flies, however, showed only partial or no protection,respectively, against paraquat-induced oxidative stress, indicating essentiality of DJ-1β for AR-mediated oxidativestress tolerance. AR feeding reduced the accumulation of reactive oxygen species (ROS) and lipid peroxidation evenin aged (35-day-old) wild-type flies while enhancing superoxide dismutase (SOD) activity. We show that while Hsp70or Hsp83 expression under normal or stress conditions was not affected by AR feeding, Hsp27 levels were elevated inAR-fed wild-type control as well as heat-shocked larvae. Therefore, besides the known anti-oxidant activity ofPhyllanthus emblica fruits, dietary AR also enhances cellular levels of Hsp27. Our in vivo study on a model organismshows that AR feeding significantly improves tolerance to a variety of cell stresses through reduced ROS and lipidperoxidation on the one hand, and enhanced SOD activity and Hsp27 on the other. The resulting better homeostasisimproves life span and quality of organism’s life.

  20. Aging Theories of TCM and Experimental Research Progress of Anti-aging%中医衰老理论与抗衰老的实验研究进展

    Institute of Scientific and Technical Information of China (English)

    李佳佳; 马健

    2013-01-01

    目的:总结中医衰老理论与抗衰老的实验研究进展.方法:收集近10 a来关于中医学衰老理论及相关中医药实验研究文献,并进行系统的归纳与总结.结果:中医衰老理论主要包括体质说、脏腑阴阳虚损致衰说、邪实致衰说、情志致衰说几方面,抗衰老中药通过现代药理实验证实疗效肯定,说明中医的相关学说有一定的科学性.结论:衰老的发生是很复杂的过程,单凭某一种理论尚无法完全阐释清楚,很多机制尚未清楚,仍需更多的研究来验证.%Objective:To explore the aging theories of TCM and experimental research progress of anti-aging. Methods:Aging theories of TCM and related traditional Chinese medical experimental research literatures in recent 10 years were collected, systematically concluded, and summarized. Results: Aging theories of TCM mainly included the aspects of theory of constitution, Viscera -Yin and Yang deficiency resulting senility, excess-evil resulting senility, emotion resulting senility. Anti-aging effects of Chinese medicinal herbs used were confirmed that they had the modern pharmacology experimental basis, which explained that related theory of TCM had certain scientifiei-ty. Conclusion: The occurrence of aging is a very complicated process, so it is impossible for a certain theory to clarify aging completely, and various mechanisms have not clear yet,which still needs more researches to verify.

  1. Mild exposure of RIN-5F β-cells to human islet amyloid polypeptide aggregates upregulates antioxidant enzymes via NADPH oxidase-RAGE: An hormetic stimulus

    Directory of Open Access Journals (Sweden)

    Elisabetta Borchi

    2014-01-01

    Full Text Available The presence of amyloid aggregates of human islet amyloid polypeptide (hIAPP, a hallmark of type 2 diabetes, contributes to pancreatic β-cell impairment, where oxidative stress plays a key role. A contribution of NADPH oxidase to reactive oxygen species (ROS generation after cell exposure to micromolar concentrations of hIAPP aggregates has been suggested. However, little is known about β-cells exposure to lower amounts of hIAPP aggregates, similar to those found in human pancreas. Thus, we aimed to investigate the events resulting from RIN-5F cells exposure to nanomolar concentrations of toxic hIAPP aggregates. We found an early and transient rise of NADPH oxidase activity resulting from increased Nox1 expression following the engagement of receptor for advanced glycation end-products (RAGE by hIAPP aggregates. Unexpectedly, NADPH oxidase activation was not accompanied by a significant ROS increase and the lipoperoxidation level was significantly reduced. Indeed, cell exposure to hIAPP aggregates affected the antioxidant defences, inducing a significant increase of the expression and activity of catalase and glutathione peroxidase. We conclude that exposure of pancreatic β-cells to nanomolar concentrations of hIAPP aggregates for a short time induces an hormetic response via the RAGE-Nox1 axis; the latter stimulates the enzymatic antioxidant defences that preserve the cells against oxidative stress damage.

  2. Evaluation of Anti-aging Compounds Using the Promoters of Elastin and Fibrillin-1 Genes Combined with a Secreted Alkaline Phosphatase Reporter in Normal Human Fibroblasts.

    Science.gov (United States)

    Lin, Chih-Chien; Yang, Chao-Hsun; Kuo, Wan-Ting; Chen, Cheng-Yu

    2015-01-01

    Elastic fibers are major constituents of the extracellular matrix (ECM) in dynamic tissues in the human body, and regulation of elastin and fibrillin-1 expression mediates the formation of these fibers. Traditional assays for the measurement of elastin and fibrillin-1, such as western blotting, Luna staining and immunostaining, are relatively complex and time-consuming. Thus, a relatively simple assay system that also provides rational results is urgently needed. In the study, we aimed to develop a human cell-based assay system that can be used to analyze functional compounds using the promoters of elastin (ELN) and fibrillin-1 (FBN1) genes integrated with a secreted alkaline phosphatase (SEAP) reporter in normal human fibroblast cells. We used this system to assess anti-aging compounds. We used several regulators of elastinogenesis, including retinol, coenzyme Q10, deoxyArbutin and Elestan(TM) (Manilkara multinervis leaf extract), to verify the efficacy of this assay system. Our results demonstrate that this assay system can be used as a fast and realistic method for identifying anti-aging components for future use in foods, cosmetics and drugs.

  3. Age transcended: a semiotic and rhetorical analysis of the discourse of agelessness in North American anti-aging skin care advertisements.

    Science.gov (United States)

    Ellison, Kirsten L

    2014-04-01

    Drawing from a collection of over 160 North American print advertisements for anti-aging skin care products from January to December of 2009, this paper examines the discourse of agelessness, a vision of esthetic perfection and optimal health that is continually referred to by gerontologists, cultural theorists, and scientific researchers as a state of being to which humankind can aspire. Employing critical discourse analysis through the use of semiotics and visual rhetoric, this paper explores the means through which anti-aging skin care advertisements present to their viewers a particular object of desire, looking, more specifically, at how agelessness is presented as a way out and ultimate transcendence of age. Through the analytical tools of semiotics and visual rhetoric, four visions of agelessness are identified and explored in this paper: Agelessness as Scientific Purity, Agelessness as Genetic Impulse, Agelessness as Nature's Essence, and Agelessness as Myth. Whether found in the heights of scientific purity, the inner core of our genetic impulse, the depths of nature's essence, or whether agelessness itself has reached its own, untouchable, mythic status, the advertisements in this study represent one of the most pervasive vehicles through which our current vision(s) of ageless perfection are reflected, reinforced, and suspended in a drop of cream.

  4. Atom probe analysis on interaction between Cr and N in bake-hardening steels with anti-aging properties at RT

    Energy Technology Data Exchange (ETDEWEB)

    Takahashi, Jun, E-mail: takahashi.3ct.jun@jp.nssmc.com [Advanced Technology Research Laboratories, Nippon Steel and Sumitomo Metal Corporation, 20-1, Shintomi, Futtsu-city, Chiba 293-8511 (Japan); Maruyama, Naoki; Kawakami, Kazuto; Yoshinaga, Naoki; Sugiyama, Masaaki [Advanced Technology Research Laboratories, Nippon Steel and Sumitomo Metal Corporation, 20-1, Shintomi, Futtsu-city, Chiba 293-8511 (Japan); Ohkubo, Tadakatsu; Ping, De-hai; Hono, Kazuhiro [National Institute for Materials Science, 1-2-1, Sengen, Tsukuba, Ibaraki 305-0047 (Japan)

    2013-11-15

    One-dimensional atom probe (1DAP) analysis was performed on chromium and nitrogen added bake hardening steel sheets that have both high bake-hardenability and anti-aging properties at room temperature (RT). The atomic data of more than 2 million atoms were collected for sample steels with and without low-temperature aging after recrystallization annealing and quenching. The correlation in atomic position between chromium and nitrogen atoms in a solid solution was investigated by a statistical analysis using the binomial distribution function. In the samples with low-temperature aging, the probability that a chromium atom was observed near a nitrogen atom was significantly higher than that estimated from the null hypothesis that there was no attractive atomic interaction. This suggests that there is an attractive interaction between a nitrogen atom and a chromium atom in bcc iron, which led to the anti-aging properties at RT. In contrast, such correlation was not observed definitely in the samples without low-temperature aging, which implied that the atomic pair formation is a thermal activation process.

  5. A steroid like phytochemical Antcin M is an anti-aging reagent that eliminates hyperglycemia-accelerated premature senescence in dermal fibroblasts by direct activation of Nrf2 and SIRT-1.

    Science.gov (United States)

    Senthil, Kumar K J; Gokila, Vani M; Mau, Jeng-Leun; Lin, Chin-Chung; Chu, Fang-Hua; Wei, Chia-Cheng; Liao, Vivian Hsiu-Chuan; Wang, Sheng-Yang

    2016-09-27

    The present study revealed the anti-aging properties of antcin M (ANM) and elucidated the molecular mechanism underlying the effects. We found that exposure of human normal dermal fibroblasts (HNDFs) to high-glucose (HG, 30 mM) for 3 days, accelerated G0/G1 phase arrest and senescence. Indeed, co-treatment with ANM (10 µM) significantly attenuated HG-induced growth arrest and promoted cell proliferation. Further molecular analysis revealed that ANM blocked the HG-induced reduction in G1-S transition regulatory proteins such as cyclin D, cyclin E, CDK4, CDK6, CDK2 and protein retinoblastoma (pRb). In addition, treatment with ANM eliminated HG-induced reactive oxygen species (ROS) through the induction of anti-oxidant genes, HO-1 and NQO-1 via transcriptional activation of Nrf2. Moreover, treatment with ANM abolished HG-induced SIPS as evidenced by reduced senescence-associated β-galactosidase (SA-β-gal) activity. This effect was further confirmed by reduction in senescence-associated marker proteins including, p21CIP1, p16INK4A, and p53/FoxO1 acetylation. Also, the HG-induced decline in aging-related marker protein SMP30 was rescued by ANM. Furthermore, treatment with ANM increased SIRT-1 expression, and prevented SIRT-1 depletion. This protection was consistent with inhibition of SIRT-1 phosphorylation at Ser47 followed by blocking its upstream kinases, p38 MAPK and JNK/SAPK. Further analysis revealed that ANM partially protected HG-induced senescence in SIRT-1 silenced cells. A similar effect was also observed in Nrf2 silenced cells. However, a complete loss of protection was observed in both Nrf2 and SIRT-1 knockdown cells suggesting that both induction of Nrf2-mediated anti-oxidant defense and SIRT-1-mediated deacetylation activity contribute to the anti-aging properties of ANM in vitro. Result of in vivo studies shows that ANM-treated C. elegens exhibits an increased survival rate during HG-induced oxidative stress insult. Furthermore, ANM significantly

  6. Research for anti-aging function of huanshaodan in aged mice induced by D-galactose%还少丹对D-半乳糖致衰小鼠的抗衰老作用

    Institute of Scientific and Technical Information of China (English)

    程莉娟; 刘群良; 谭峰; 张波; 陈伶利

    2012-01-01

    目的 探讨还少丹对D-半乳糖(Dgalactose,D-gal)诱导的衰老模型小鼠的抗衰老作用.方法 采用D-gal建立小鼠衰老模型,用还少丹水煎剂灌胃6周;分光光度法检测小鼠脑、肝脏组织谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)和超氧化物歧化酶(superoxidase dismutase,SOD)活性;观察小鼠肝脏、脾脏指数的改变.结果 衰老模型组小鼠肝脏指数、脾脏指数以及脑和肝脏组织的SOD、GSH-Px活性均明显降低.还少丹能显著提高衰老模型小鼠的肝脏指数和脾脏指数;显著升高脑和肝组织的SOD、GSH-Px活性.结论 还少丹的抗衰老作用与其影响器官指数、升高GSH-Px和SOD活性有关,其作用机制可能通过清除体内自由基,提高免疫功能以实现延缓衰老的进程.%Objective To investigate the effect and mechanism of huanshaodan on anti-aging in aged mice induced by D-galactose(D-gal). Methods The aged model was established by injecting D-gal. After feeding with huanshaodan for 6 weeks, spectrophotometry was used to detect the activity of glutathione peroxidase and superoxidase dismutase in the brain and liver of mice. Meanwhile the liver index and spleen index of mice were also detected. Results The liver index, spleen index, and the activity of glutathione peroxidase and superoxidase dismutase of brain and liver in the aged model group were significantly reduced, whereas the liver index, spleen index.the activity of superoxidase dismutase and glutathione peroxidase in the brain and liver were elevated remarkably. Conclusion The activity of glutathione peroxidase and superoxidase dismutase in the brain and liver of aged mice can be elevated by huanshaodan, and so do the liver index and spleen index, which suggest that huanshaodan has an anti-aging effect through eliminating free radicals and improving the immunity.

  7. Secretion expression of SOD1 and its overlapping function with GSH in brewing yeast strain for better flavor and anti-aging ability.

    Science.gov (United States)

    Wang, Zhaoyue; Bai, Xuejing; He, Xiuping; Zhang, Borun

    2014-09-01

    Superoxide dismutase (SOD) is a significant antioxidant, but unlike glutathione (GSH), SOD cannot be secreted into beer by yeast cells during fermentation, this directly leads to the limited application of SOD in beer anti-aging. In this investigation, we constructed the SOD1 secretion cassette in which strong promoter PGK1p and the sequence of secreting signal factor from Saccharomyces cerevisiae were both harbored to the upstream of coding sequence of SOD1 gene, as a result, the obtained strains carrying this cassette successfully realized the secretion of SOD1. In order to overcome the limitation of previous genetic modification on yeast strains, one new comprehensive strategy was adopted targeting the suitable homologous sites by gene deletion and SOD1 + GSH1 co-overexpression, and the new strain ST31 (Δadh2::SOD1 + Δilv2::GSH1) was constructed. The results of the pilot-scale fermentation showed that the diacetyl content of ST31 was lower by 42 % than that of the host, and the acetaldehyde content decreased by 29 %, the GSH content in the fermenting liquor of ST31 increased by 29 % compared with the host. Both SOD activity test and the positive and negative staining assay after native PAGE indicated that the secreted active SOD in the fermenting liquor of ST31 was mainly a dimer with the size of 32,500 Da. The anti-aging indexes such as the thiobarbituric acid and the resistance staling value further proved that the flavor stability of the beer brewed with strain ST31 was not only better than that of the original strain, but also better than that of the previous engineering strains. The multi-modification and comprehensive improvement of the beer yeast strain would greatly enhance beer quality than ever, and the self-cloning strain would be attractive to the public due to its bio-safety.

  8. Stress to the Rescue: Is Hormesis a ‘Cure’ For Aging?

    DEFF Research Database (Denmark)

    Kahn, Arnold; Olsen, Anders

    2009-01-01

    Despite the fact that the phenomenon of hormesis has been known for many years it is still very much an area of controversy just how useful hormetic treatments are in preventing age-related human diseases and increasing life expectancy. Since there are no data in humans demonstrating hormesis as ...... as an effective anti-ageing strategy we turn to a simple model organism for insight. In this review we explore what can be predicted about the usefulness of hormetic treatments in humans based upon studies conducted in the soil nematode Caenorhabditis elegans.......Despite the fact that the phenomenon of hormesis has been known for many years it is still very much an area of controversy just how useful hormetic treatments are in preventing age-related human diseases and increasing life expectancy. Since there are no data in humans demonstrating hormesis...

  9. Hormesis in aging.

    Science.gov (United States)

    Rattan, Suresh I S

    2008-01-01

    Hormesis in aging is represented by mild stress-induced stimulation of protective mechanisms in cells and organisms resulting in biologically beneficial effects. Single or multiple exposure to low doses of otherwise harmful agents, such as irradiation, food limitation, heat stress, hypergravity, reactive oxygen species and other free radicals have a variety of anti-aging and longevity-extending hormetic effects. Detailed molecular mechanisms that bring about the hormetic effects are being increasingly understood, and comprise a cascade of stress response and other pathways of maintenance and repair. Although the extent of immediate hormetic effects after exposure to a particular stress may only be moderate, the chain of events following initial hormesis leads to biologically amplified effects that are much larger, synergistic and pleiotropic. A consequence of hormetic amplification is an increase in the homeodynamic space of a living system in terms of increased defence capacity and reduced load of damaged macromolecules. Hormetic strengthening of the homeodynamic space provides wider margins for metabolic fluctuation, stress tolerance, adaptation and survival. Hormesis thus counter-balances the progressive shrinkage of the homeodynamic space, which is the ultimate cause of aging, diseases and death. Healthy aging may be achieved by hormesis through mild and periodic, but not severe or chronic, physical and mental challenges, and by the use of nutritional hormesis incorporating mild stress-inducing molecules called hormetins. The established scientific foundations of hormesis are ready to pave the way for new and effective approaches in aging research and intervention.

  10. Simultaneous determination of 30 hormones illegally added to anti-ageing functional foods using UPLC-MS/MS coupled with SPE clean-up.

    Science.gov (United States)

    He, Xiaoqin; Xi, Cunxian; Tang, Bobin; Wang, Guomin; Chen, Dongdong; Peng, Tao; Mu, Zhaode

    2014-01-01

    A novel analytical method employing solid-phase extraction (SPE) coupled with ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was developed for the simultaneous determination of 30 hormones in anti-ageing functional foods (capsules, powders and tablets). The analytes were extracted with acetic acid-acetonitrile (1-99 v/v), methanol and acetone, respectively. The extract was purified using a combined column, followed by analyte detection with electrospray ionisation in positive- or negative-ion modes. The results indicated that the 30 compounds had good linear correlations in the range of 1-1000 μg kg⁻¹, and the correlation coefficients were above 0.99. The limits of detection (LOD) and limits of quantification (LOQ) were 0.03-2 and 0.1-5 μg kg⁻¹, respectively. The average recovery of 30 compounds at the three spiked levels varied from 74.7% to 124.1%, and the relative standard deviation (RSD) was 2.4-15.0%. This method was applied to the analysis of hormones in 14 real samples of which seven hormones (such as estrone, dienestrol) were detected in four samples, but the remainder of the hormones were not detected. The developed method is sensitive, efficient, reliable and applicable to real samples.

  11. Embryonic anti-aging niche.

    Science.gov (United States)

    Conboy, Irina M; Yousef, Hanadie; Conboy, Michael J

    2011-05-01

    Although functional organ stem cells persist in the old, tissue damage invariably overwhelms tissue repair, ultimately causing the demise of an organism. The poor performance of stem cells in an aged organ, such as skeletal muscle, is caused by the changes in regulatory pathways such as Notch, MAPK and TGF-β, where old differentiated tissue actually inhibits its own regeneration. This perspective analyzes the current literature on regulation of organ stem cells by their young versus old niches and suggests that determinants of healthy and prolonged life might be under a combinatorial control of cell cycle check point proteins and mitogens, which need to be tightly balanced in order to promote tissue regeneration without tumor formation. While responses of adult stem cells are regulated extrinsically and age-specifically, we put forward experimental evidence suggesting that embryonic cells have an intrinsic youthful barrier to aging and produce soluble pro-regenerative proteins that signal the MAPK pathway for rejuvenating myogenesis. Future identification of this activity will improve our understanding of embryonic versus adult regulation of tissue regeneration suggesting novel strategies for organ rejuvenation. Comprehensively, the current intersection of aging and stem cell science indicates that if the age-imposed decline in the regenerative capacity of stem cells was understood, the debilitating lack of organ maintenance in the old could be ameliorated and perhaps, even reversed.

  12. Anti-aging Decoction Influence Kidney Aging-concerned Gene Expression of Rats of Blood Stasis%抗衰汤对血瘀证大鼠肾脏衰老相关基因表达的影响

    Institute of Scientific and Technical Information of China (English)

    何赛萍; 徐莉

    2011-01-01

    [目的]探讨血瘀与衰老的相关性、抗衰汤抗衰老的分子生物学机制,以利中药抗衰老制剂的开发.[方法]使用基因芯片技术,确定血瘀影响的衰老基因表达,并考核抗衰汤对血瘀证大鼠衰老相关基因表达谱的影响.[结果]确定16个基因为与血瘀相关的衰老基因,但其表达水平受到抗衰汤用药的影响.[结论]抗衰汤对与血瘀相关的衰老基因有明显地调控作用,血瘀是导致衰老的关键因素之一.%[Objective] To explore relativity between blood stasis and aging, molecular mechanism of Anti-aging Decoction, for development of anti-aging TCM preparation. [Method] Apply gene chip technology, define aging gene expression affected by blood stasis, check the influence of Anti-aging Decoction on senile rats' concerned gene expression spectrum. [Result] It defines that 16 genes are related with aging, while the expression level is influenced with Anti-aging Decoction application. [Conclusion] Anti-aging Decoction has marked regulation to senile gene related with blood stasis; blood stasis is one key factor of senium.

  13. Research progress of Anti-aging and Anti-tumor TCM Drugs on Telomere, Telomerase%抗衰老及抗肿瘤中药对端粒、端粒酶影响的研究进展

    Institute of Scientific and Technical Information of China (English)

    李桂霞; 王明艳; 高书亮

    2011-01-01

    Since the Nobel Prize in medicine was awarded the scholars who found telomere and telomerase in 2009,the researches for telomere and telomerase become a focus again. This paper reviews the influence of telomere length and telomerase activity by the traditional Chinese medicine of anti - aging and anti - tumor,providing new ideas of the research direction for the traditional medicine of anti - aging and anti - tumor.%自2009年诺贝尔医学奖被授予发现端粒和端粒酶的学者后,端粒、端粒酶的研究又成为热点.文章综述了抗衰老及抗肿瘤中药对细胞端粒长短和端粒酶活性影响的研究,提出了抗衰老及抗肿瘤中药研究方向的新思路.

  14. 新的衰老分子机制与抗衰老新药的开发%New Molecular Mechanism of Aging and Development of Anti-aging Drugs

    Institute of Scientific and Technical Information of China (English)

    陈执中

    2011-01-01

    目的 抗衰老健康长寿是人类的美好愿望.衰老机制的研究与抗衰老新药的开发是生命科学领域中的一个重要课题,也是21世纪研究的热点之一.本文讨论了端粒对寿命的影响,综述了sirtuin家族中两个成员--SIRT1和SIRT6的作用机制的进展和抗衰老新药的开发.衰老分子机制的破解为延缓衰老、促进健康提供了理论基础,为抗衰老新药的研究开发开拓广阔的前景.%Objective The anti-aging, long life and health are fine aspiration of mankind. Study on the mechanisms of aging and development of anti-aging drugs are an important subject in the life science field, and also are one of research hot spots in great demand in 21 century. In this article, the influence on life span by telomere is discussed and the progress on the mechanisms of two members of sirtuin family-SIRTl and SIRT6 and the development of antiaging drugs are reviewed. Getting to heart of molecular mechanism of aging provides a theoretical basis of anti-aging and health, meanwhile, it opens up a broad prospect for the development of anti-aging drugs.

  15. 秀丽隐杆线虫在抗衰老领域应用的研究进展%Advances in researches on the anti-aging of Caenorhabditis elegans

    Institute of Scientific and Technical Information of China (English)

    游牧(综述); 胡云虎(审校)

    2016-01-01

    秀丽隐杆线虫(Caenorhabditis elegans)是抗衰老研究中重要的模式生物之一,被广泛应用于抗衰老物质的筛选和抗衰老分子机制的研究中。本文回顾了近年来秀丽隐杆线虫在抗衰老领域应用的研究进展,介绍了抗衰老物质的筛选情况,系统综述了作为抗衰老物质筛选评价的生理指标和反映抗衰老机制的生化指标包括寿命、生存率、生存时间、ROS含量、脂褐质积累以及daf-16,sod-3,gst-4,hsps基因调控情况等,指出了研究中存在的问题,展望了秀丽隐杆线虫在抗衰老研究中的应用前景。%Caenorhabditis elegans is one of the most important model organisms,and is widely used in evaluation of anti-aging substance and mechanism study.This article reviewed the application of Caenorhabditis elegans research progress in the ifeld of anti-aging in recent years,introduced the screening of anti-aging substance,summarized some indexes such as lifespan, survival rates,survival time, ROS,lipofuscin,daf-16,sod-3,gst-4 and hsps.The unresolved problems were pointed out and the possible ways to solve these problems were expounded.Finally a prospect of the application in anti-aging research was analyzed.This paper can provide useful information for researchers in related ifelds.

  16. 抗老化剂改善竹粉/聚丙烯发泡复合材料的自然老化性能%Anti-aging agents improving natural weathering properties of bamboo powder/polypropylene foamed composites

    Institute of Scientific and Technical Information of China (English)

    周吓星; 陈礼辉

    2015-01-01

    production, the natural weathering performance of bamboo powder/polypropylene (PP) foamed composites was researched. The composites containing 33% bamboo flour, 1% modified AC (azodicarbonamide) foaming agent and different anti-aging agents were prepared by the injection molding. The composites were exposed to outdoor natural weathering from October 2012 to October 2013 in Fuzhou (26°08′N, 119°18′E), the southeast of China. The effects of natural weathering on the composites’ color, flexural creep behavior and rheological behavior were investigated. Meanwhile, the effects of the antioxidant B225 and anti-ultraviolet agent UV770 on the composites’ weathering performance were also studied. The B225 is a complex antioxidant agent using Tetra-[methylene-β-(3, 5-di-tert-butyl-4-hydroxyphenyl)-propionate] methan and Tns-(2, 4-di-tert-butyl)-phosphite, with a ratio of 1:1. The UV770 is bis(2,2,6,6-tetramethyl-4-piperidyl)sebacate. The results showed that both the B225 and the UV770 could slow down the color fading and improve the color stability during weathering. After weathering for 12 months, the color change reduced from22.8 for the composites without adding antioxidant to 14.2, 17.6 and 16.8 for the composites contained with 0.6% B225, 0.6% UV770, 0.3% B225 and 0.3% UV770, respectively. The B225 has more positive effect on color stability of the composites than UV770. According to authors’ prior research, the influence of natural weathering on the composites’ color was similar with the artificial accelerated weathering. The color change caused by the natural weathering for 12 months was equal to that by accelerated weathering of xenon-arc radiation with water spray for 0.4 months, and also was similar to that by the accelerated cycling of water immersion followed by freeze-thaw cycle at the temperature of -40-60℃for 22 times. Due to the combined effect of the creep and long-term weathering, particularly at 75% stress level, the creep strain increased, and the

  17. An Experimental Study on Anti-aging Effects of Resveratrol%白藜芦醇抗衰老作用的实验研究

    Institute of Scientific and Technical Information of China (English)

    王长本; 李良琼; 孟凡萍

    2009-01-01

    目的:探讨白藜芦醇的抗衰老作用.方法:采用避暗实验观察白藜芦醇对小鼠学习记忆的影响.通过常压耐缺氧、断头耐缺氧、亚硝酸钠中毒造成的缺氧以及负重游泳实验,观察白藜芦醇对小鼠缺氧及耐力的影响;以AlCl3造成急性衰老小鼠模型,观察白藜芦醇对小鼠血中超氧化物歧化酶(SOD)活性的影响.结果:白藜芦醇各剂量组均能明显改善记忆障碍,与模型组比较差异有显著性(P<0.01).在耐力实验中,白藜芦醇可显著延长小鼠缺氧时间,与溶剂组比较有显著性差异(P<0.05).结论:白藜芦醇具有一定的抗衰老作用.

  18. Anti-Ageing Effects of Sonchus oleraceus L. (pūhā) Leaf Extracts on H2O2-Induced Cell Senescence

    OpenAIRE

    Zong-Quan Ou; Thomas Rades; Arlene McDowell

    2015-01-01

    Antioxidants protect against damage from free radicals and are believed to slow the ageing process. Previously, we have reported the high antioxidant activity of 70% methanolic Sonchus oleraceus L. (Asteraceae) leaf extracts. We hypothesize that S. oleraceus extracts protect cells against H2O2-induced senescence by mediating oxidative stress. Premature senescence of young WI-38 cells was induced by application of H2O2. Cells were treated with S. oleraceus extracts before or after H2O2 stress...

  19. Advances in the Study of Aging and Anti-aging of Long Glass Fiber Reinforced Composites%长玻纤增强复合材料老化研究进展及防老化研究

    Institute of Scientific and Technical Information of China (English)

    左晓玲; 张道海; 罗兴; 吴斌; 郝智; 郭建兵

    2013-01-01

    综述了以热塑性材料为基体树脂的长玻纤增强复合材料结构分析,阐述了长玻纤增强热塑性复合材料在湿热、热氧、光氧条件下有可能的老化机理.最后提出了几点长玻纤增强热塑性复合材料防老化的建议措施.%The structure analysis of thermoplastic materials for the matrix resin of long glass fiber reinforced composites was summarized, the possible aging mechanisms of long glass fiber reinforced thermoplastic composites in hygrotherma, thermal oxidation and light conditions were elaborated. Finally several measures in anti-aging research for long glass fiber reinforced thermoplastic composites were proposed.

  20. Anti-elastase, anti-tyrosinase and matrix metalloproteinase-1 inhibitory activity of earthworm extracts as potential new anti-aging agent

    Institute of Scientific and Technical Information of China (English)

    Nurhazirah Azmi; Puziah Hashim; Dzulkifly M Hashim; Normala Halimoon; Nik Muhamad Nik Majid

    2014-01-01

    Objective: To examine whether earthworms of Eisenia fetida, Lumbricus rubellus and Eudrilus eugeniae extracts have elastase, tyrosinase and matrix metalloproteinase-1 (MMP-1) inhibitory activity.Methods:activity and compared with the positive controls. It was also evaluated for whitening and anti-wrinkle capacity.Results:The earthworms extract was screened for elastase, tyrosinase and MMP-1 inhibitory and excellent MMP-1 inhibition compared to N-Isobutyl-N-(4-methoxyphenylsulfonyl)-glycylhydroxamic acid.Conclusions:Earthworms extract showed effective inhibition of tyrosinase, elastase and MMP-1 The extract showed significantly (P<0.05) good elastase and tyrosinase inhibition activities. Therefore, this experiment further rationalizes the traditional use of this worm extracts which may be useful as an anti-wrinkle agent.

  1. Ambient aging of rhenium filaments used in thermal ionization mass spectrometry: Growth of oxo-rhenium crystallites and anti-aging strategies

    Directory of Open Access Journals (Sweden)

    Joseph M. Mannion

    2017-01-01

    Full Text Available Degassing is a common preparation technique for rhenium filaments used for thermal ionization mass spectrometric analysis of actinides, including plutonium. Although optimization studies regarding degassing conditions have been reported, little work has been done to characterize filament aging after degassing. In this study, the effects of filament aging after degassing were explored to determine a “shelf-life” for degassed rhenium filaments, and methods to limit filament aging were investigated. Zone-refined rhenium filaments were degassed by resistance heating under high vacuum before exposure to ambient atmosphere for up to 2 months. After degassing the nucleation and preferential growth of oxo-rhenium crystallites on the surface of polycrystalline rhenium filaments was observed by atomic force microscopy and scanning electron microscopy (SEM. Compositional analysis of the crystallites was conducted using SEM-Raman spectroscopy and SEM energy dispersive X-ray spectroscopy, and grain orientation at the metal surface was investigated by electron back-scatter diffraction mapping. Spectra collected by SEM-Raman suggest crystallites are composed primarily of perrhenic acid. The relative extent of growth and crystallite morphology were found to be grain dependent and affected by the dissolution of carbon into filaments during annealing (often referred to as carbonization or carburization. Crystallites were observed to nucleate in region specific modes and grow over time through transfer of material from the surface. Factors most likely to affect the rates of crystallite growth include rhenium substrate properties such as grain size, orientation, levels of dissolved carbon, and relative abundance of defect sites; as well as environmental factors such as length of exposure to oxygen and relative humidity. Thin (∼180 nm hydrophobic films of poly(vinylbenzyl chloride were found to slow the growth of oxo-rhenium crystallites on the filament

  2. A Narrative Approach to Anti-Aging.

    Science.gov (United States)

    Ray, Ruth E.; Chandler, Sally

    2002-01-01

    Reminiscence functions in multiple ways. Reminiscence that is received positively and that is not associated with a negative old age tells a story in progress, still growing and evolving through the process of repetition. (Author/JOW)

  3. [Epidermal aging and anti-aging strategies].

    Science.gov (United States)

    Wohlrab, J; Hilpert, K; Wolff, L

    2016-02-01

    Epithelial senescence is a complex process depending on intrinsic as well as extrinsic factors (e.g., UV or IR light, tobacco smoke) and must be seen in the context of the aging process especially of the corium and the subcutis. Morphological alterations become apparent in the form of epithelial atrophy, structural changes within the basal membrane, and a decrease in cell count of melanocytes and Langerhans cells. Signs of cellular senescence are reduced proliferation of keratinocytes, cumulation of dysplastic keratinocytes, various mutations (e.g., c-Fos/c-Jun, STAT3, FoxO1), as well as multiple lipid or amino acid metabolic aberrations (e.g., production of advanced glycation endproducts). This causes functional changes within the physical (lipid deficiency, water distribution dysfunction, lack of hygroscopic substances), chemical (pH conditions, oxygen radicals), and immunological barrier. Prophylactically, barrier-protective care products, antioxidant substances (e.g., vitamin C, B3, E, polyphenols, flavonoids), sunscreen products/measurements, and retinoids are used. For correcting alterations in aged epidermis, chemical peelings (fruit acids, β-hydroxy acid, trichloroacetic acid, phenolic compounds), non-ablative (IPL, PDL, Nd:YAG) as well as ablative (CO2, Erbium-YAG) light-assisted methods are used.

  4. Neuropeptide Y: An Anti-Aging Player?

    Science.gov (United States)

    Botelho, Mariana; Cavadas, Cláudia

    2015-11-01

    Accumulating evidence suggests that neuropeptide Y (NPY) has a role in aging and lifespan determination. In this review, we critically discuss age-related changes in NPY levels in the brain, together with recent findings concerning the contribution of NPY to, and impact on, six hallmarks of aging, specifically: loss of proteostasis, stem cell exhaustion, altered intercellular communication, deregulated nutrient sensing, cellular senescence, and mitochondrial dysfunction. Understanding how NPY contributes to, and counteracts, these hallmarks of aging will open new avenues of research on limiting damage related to aging.

  5. Study on the Relationship between the Anti-aging Function and Antioxidation of Ethanol Extract from Angelicoe Dahuricae Radix%白芷醇提物延缓皮肤衰老与抗氧化作用的相关性研究

    Institute of Scientific and Technical Information of China (English)

    王方; 王灿

    2012-01-01

    days. 3 antioxidation index and 2 skin aging index in mice skin homogenate were determined, and correlation analysis of them was conducted. RESULTS: Ethanol extract from Angelicae Dahuricae Radix showed significant clearance effects on 3 free radicals in concentration-dependent manner. Also, the high and medium doses of the extract could significantly increase the activity of SOD, reduce the content of MDA and lipofuscin(LF)in the skin of the mice, meanwhile, they could significantly increase the content of hydroxyproline(Hyp)and moisture (P<0.01 or P<0.05); the low dose could increase the activity of SOD, reduce the content of MDA, and could increase the content of moisture in the mice skin(P<0.05). There was a good correlation between the effects of high dose of ethanol extract from Angelicae Dahuricae Radix on Hyp and that of oxidative index such as SOD,MDA,LF (determining coefficient r=0.921). CONCLUSION:Ethanol extract from Angelicae Dahuricae Radix has strong an-tioxidative effects both in vitro and in vivo. There is a good correlation between its antioxidative function and its anti-aging function to hint that its antioxidative activity could be one of the whitening anti-aging mechanisms.

  6. Anti-Aging Function of the Blended Essential Oil in Murine Aging Model Induced by Galactose%复方精油对D-半乳糖衰老模型小鼠的抗衰老作用

    Institute of Scientific and Technical Information of China (English)

    黄玲玲; 魏道智; 吴亚妮; 姚雷

    2012-01-01

    To investigate the anti-aging function of the blended essential oil in murine aging model, mice were injected subcutaneously with Dgalactose and the blended essential oil with 3 different concentrations was applied by smearing the mixture on the naked back skin of the mice for 42 days. The serum and skin specimen were collected to determine the activity of superoxide dismutase (SOD), the content of malondialdehyde (MDA) and hydroxyproline (HYP). The results showed that, compared with model group without treatment of essential oil, the SOD activity in the serum of the three groups treated with high,medium and low dose of blended essential oil was significantly improved by 14. 94%~20. 27% (P< 0. 01) and the MDA content was decreased by 17. 00%~26. 30% (P<0. 01 or P<0. 05). In the skin specimen,on the other hand,the activity of SOD was elevated by 58. 00%~61. 11% (P<0. 01) and the content of MDA decreased by 12. 23%~26. 04% (P<0. 01) while the HYP level was increased 33. 70%~ 140. 70% (P<0. 01). In conclusion, the blended essential oil possesses satisfying function of anti-oxidation and anti-aging,indicating potential usage in the future.%探讨复方精油对D-半乳糖衰老模型小鼠的抗衰老作用.通过皮下注射D-半乳糖制备小鼠衰老模型,同时小鼠背部脱毛并连续涂抹3个不同浓度的复方精油42 d,取小鼠血清及涂药处皮肤检测超氧化物歧化酶(SOD)的活性和丙二醛(MDA)含量,同时检测皮肤中羟脯氨酸(HYP)含量.结果表明,与模型组比较,高中低浓度复方精油组小鼠血清中SOD活性提高14.94%~20.27% (P<0.01),MDA含量降低17.00%~26.30% (P<0.01或P<0.05);皮肤中SOD活性升高58.00%~61.11% (P<0.01),MDA含量降低12.23%~26.04% (P<0.01),HYP含量升高33.70%~140.70% (P<0.01).该复方精油具有良好的抗氧化、抗衰老作用,具有潜在的开发应用价值.

  7. Research on the Molecule System of Anti-aging-heart by Sports through Using Drosophila Animal Model%利用果蝇动物模型研究运动抗心脏衰老的分子机制

    Institute of Scientific and Technical Information of China (English)

    吴越; 郑澜

    2015-01-01

    Sports and training based on proper rules can slow the physiological decline of cardiac function, but it's molecule system is unknown. Currently, there are various problems in the use of mammal model to research the system. Short life cycle, simple genetic background, and mature transgenic technology of drosophila, make it an ideal model for aging gene screening and function research, which benefits from the development of the anti-gravity climbing system of drosophila in recent years. Rely-ing on previous experiment results, this paper will elaborate the feasibility and construction method of drosophila movement mod-el, and combined with the testing technology of drosophila car-diac function, introduce the development prospect and limitation of the molecule system of anti-aging-heart by sports through us-ing drosophila movement model.%适宜规律的运动训练可以延缓心脏功能的生理性衰退,但其分子机制未知。目前采用哺乳动物模型研究这一机制存在诸多困境。果蝇的生命周期短、遗传背景简单、转基因技术成熟,一直是衰老基因筛选和功能研究的理想模式生物,得益于近年果蝇抗重力攀爬系统的开发,本文将依托前期实验成果阐述果蝇运动模型的建立方法与可行性,同时结合果蝇心脏功能检测技术说明果蝇运动模型研究抗心脏衰老分子机制的发展前景和局限性。

  8. An "enigmatic" L-carnosine (β-alanyl-L-histidine)? Cell proliferative activity as a fundamental property of a natural dipeptide inherent to traditional antioxidant, anti-aging biological activities: balancing and a hormonally correct agent, novel patented oral therapy dosage formulation for mobility, skeletal muscle power and functional performance, hypothalamic-pituitary- brain relationship in health, aging and stress studies.

    Science.gov (United States)

    Babizhayev, Mark A; Yegorov, Yegor E

    2015-01-01

    Hypothalamic releasing and inhibiting hormones are major neuroendocrine regulators of human body metabolism being driven directly to the anterior pituitary gland via hypothalamic-hypophyseal portal veins. The alternative physiological or therapeutic interventions utilizing the pharmaco-nutritional boost of imidazole-containing dipeptides (non-hydrolized oral form of carnosine, carcinine, N-acetylcarnosine lubricant eye drops) can maintain health, enhance physical exercise performance and prevent ageing. Carnosine (β-alanyl-L-histidine) is synthesized in mammalian skeletal muscle. There is an evidence that the release of carnosine from the skeletal muscle sarcomeres moieties during physical exercise affects autonomic neurotransmission and physiological functions. Carnosine released from skeletal muscle during exercise acts as a powerful afferent physiological signaling stimulus for hypothalamus, may be transported into the hypothalamic tuberomammillary nucleus (TMN), specifically to TMN-histamine neurons and hydrolyzed herewith via activities of carnosine-degrading enzyme (carnosinase 2) localized in situ. Through the colocalized enzymatic activity of Histidine decarboxylase in the histaminergic neurons, the resulting L-histidine may subsequently be converted into histamine, which could be responsible for the effects of carnosine on neurotransmission and physiological function. Carnosine and its imidazole-containing dipeptide derivatives are renowned for their anti-aging, antioxidant, membrane protective, metal ion chelating, buffering, anti-glycation/ transglycating activities used to prevent and treat a spectrum of age-related and metabolic diseases, such as neurodegenerative disease, sight threatening eye diseases, Diabetes mellitus and its complications, cancers and other disorders due to their wide spectrum biological activities. The precursor of carnosine (and related imidazole containing compounds) synthesis in skeletal muscles beta-alanine is used as the

  9. Mitochondrial stress extends lifespan in C. elegans through neuronal hormesis.

    Science.gov (United States)

    Maglioni, Silvia; Schiavi, Alfonso; Runci, Alessandra; Shaik, Anjumara; Ventura, Natascia

    2014-08-01

    Progressive neuronal deterioration accompanied by sensory functions decline is typically observed during aging. On the other hand, structural or functional alterations of specific sensory neurons extend lifespan in the nematode Caenorhabditis elegans. Hormesis is a phenomenon by which the body benefits from moderate stress of various kinds which at high doses are harmful. Several studies indicate that different stressors can hormetically extend lifespan in C. elegans and suggest that hormetic effects could be exploited as a strategy to slow down aging and the development of age-associated (neuronal) diseases in humans. Mitochondria play a central role in the aging process and hormetic-like bimodal dose-response effects on C. elegans lifespan have been observed following different levels of mitochondrial stress. Here we tested the hypothesis that mitochondrial stress may hormetically extend C. elegans lifespan through subtle neuronal alterations. In support of our hypothesis we find that life-lengthening dose of mitochondrial stress reduces the functionality of a subset of ciliated sensory neurons in young animals. Notably, the same pro-longevity mitochondrial treatments rescue the sensory deficits in old animals. We also show that mitochondrial stress extends C. elegans lifespan acting in part through genes required for the functionality of those neurons. To our knowledge this is the first study describing a direct causal connection between sensory neuron dysfunction and extended longevity following mitochondrial stress. Our work supports the potential anti-aging effect of neuronal hormesis and open interesting possibility for the development of therapeutic strategy for age-associated neurodegenerative disorders.

  10. The anti-aging study of human umbilical cord mesenchymal stem cells combined with lycopene treatment in the aging beagles%人脐带间充质干细胞联合番茄红素延缓比格犬的自然衰老

    Institute of Scientific and Technical Information of China (English)

    王琳琳; 崔晓兰; 时瀚; 成雪; 刘佳; 申义; 李倩倩; 王意忠

    2014-01-01

    BACKGROUND:Previous tudies have shown that the anti-aging effects of stem cel s with lycopene are more significant, and can also significantly improve the aging body immune function. OBJECTIVE:To investigate the anti-aging effects of human umbilical cord mesenchymal stem cel s combined with lycopene on the aging beagles. METHODS:Sixteen aging beagles (6-7 years old) were randomly divided into two groups:aging control group and aging treatment group;young beagles (3-4 years old) were chosen as young control group. In the aging treatment group, human umbilical cord mesenchymal stem cel s combined with lycopene was given;while in the other two group, an equal amount of DMEM/F12 cel culture medium and sunflower oil was given. Each dog's general conditions were observed regularly during the whole progress. The changes of superoxide dismutase, malondialdehyde, glutathione peroxidase in the serum were detected at regular time of the whole process, and the structure changes of each organ were observed at 24 weeks of treatment. RESULTS AND CONCLUSION:(1) Before treatment, the levels of superoxide dismutase and glutathione peroxidase in the aging control and treatment groups were lower than those in the young control group (P0.05). (2) For the aging treatment group at 24 weeks of treatment:the beagle fur became clearer and smoother, motility was strengthened, appetite became better;and the activity of superoxide dismutase in serum at 8 to 24 weeks of treatment increased significantly compared with before treatment (P  目的:观察人脐带间充质干细胞联合番茄红素对自然衰老比格犬的抗衰老作用。  方法:选用自然衰老的健康比格犬(6至7岁龄)16只,随机分为衰老对照组和衰老治疗组,以健康青年犬(3至4岁龄)作为青年对照组。衰老治疗组给予人脐带间充质干细胞和番茄红素联合治疗,其余两组给予等量的DMEM/F12细胞培养液和等量葵花籽油。治疗过程中定期观察

  11. EFFECT OF LYCOPENE BY SUPERCRITICAL CO2 EXTRACTION ON ANTI-AGING%超临界CO2提取的番茄红素对衰老的作用

    Institute of Scientific and Technical Information of China (English)

    王晓岑; 于国萍; 娄毅

    2007-01-01

    [目的]研究应用超临界CO2提取的番茄红素对衰老的作用.[方法]在小鼠颈背部皮下注射D-半乳糖制成衰老模型,同时给予不同剂量的番茄红素灌喂,8周后,检测小鼠血浆、肝脏组织中的超氧化物歧化酶(SOD)、丙二醛(MDA)、谷光甘肽过氧化物酶(GSH-Px).[结果]适量的番茄红素能显著提高D-半乳糖致衰老模型小鼠血浆、肝脏中SOD、GSH-Px活力,显著降低模型小鼠血浆、肝脏中MDA含量.[结论]超临界CO2提取的番茄红素有良好的抗衰老作用.

  12. Protective Effects on Mitochondria and Anti-Aging Activity of Aqueous Extract of Forsythia suspensa Leavies%连翘叶水提物保护线粒体及抗衰老研究

    Institute of Scientific and Technical Information of China (English)

    李兴泰; 陈瑞; 高明波

    2009-01-01

    研究连翘叶水提物(FLA)对自由基所致的线粒体损伤的保护作用,并研究其抗氧化能力、活性氧的清除活性及抗衰老机制.采用硫代巴比妥酸(TBA)比色法测定丙二醛(MDA)含量、分光光度法测线粒体的肿胀度,并以还原型辅酶Ⅰ-氮蓝四唑-吩嗪硫酸甲酯(NADH-NBT-PMS)为超氧阴离子生成系统测定对超氧阴离子的清除能力.利用钼酸胺比色法、黄嘌呤氧化酶法、二硫代二硝基苯甲酸比色法、Fenton反应显色法分别测定FLA对小鼠肝匀浆过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)及抗羟自由基活力的影响.FLA可明显抑制线粒体的损伤及线粒体肿胀,并呈剂量依赖关系,清除超氧阴离子的能力显著,且能明显增强CAT、SOD、GSH-Px及抗羟自由基活力.FLA能通过清除活性氧自由基及提高抗氧化酶活力来保护线粒体,具有一定的抗氧化及抗衰老药用价值.

  13. Anti-Ageing Effects of Sonchus oleraceus L. (pūhā) Leaf Extracts on H2O2-Induced Cell Senescence

    DEFF Research Database (Denmark)

    Ou, Zong-Quan; Rades, Thomas; McDowell, Arlene

    2015-01-01

    Antioxidants protect against damage from free radicals and are believed to slow the ageing process. Previously, we have reported the high antioxidant activity of 70% methanolic Sonchus oleraceus L. (Asteraceae) leaf extracts. We hypothesize that S. oleraceus extracts protect cells against H2O2...

  14. The anti-aging effect of Semen Cannabis oil on the sex difference of Bombyx mori L.%火麻仁油在家蚕抗衰老实验中的性别差异的研究

    Institute of Scientific and Technical Information of China (English)

    吴宿慧; 郭媛媛; 张神涛; 杨阳; 王瑞; 张旭屹; 李寒冰

    2015-01-01

    目的:性别差异在衰老研究中备受关注,本实验通过研究火麻仁油对雌雄家蚕寿命的延长,探究其抗衰老作用的性别差异.方法:取龄期相同、生长整齐的三眠蚕随机分为5组,即空白对照组、火麻仁油0.54 g/ml剂量组、0.27 g/ml剂量组、0.14g/ml剂量组,七宝美髯丸0.20g/ml剂量组(阳性对照),每组60条,雌雄各半.使用涂抹法将不同浓度药液涂抹于新鲜桑叶上,4龄期内喂食3次,5龄期内喂食6次,并监测不同龄期的生长天数.结果:家蚕幼虫期结果显示,在各给药组间有极显著性差异(F(4,299)=39.568,P<0.01),雌雄间亦有显著性差异(F(4,299) =7.851,P<0.05),且分组与性别之间有显著的交互作用(F(4,299)=2.748,P <0.05),但蛹期及蛾期仅有性别差异.从家蚕寿命的延长率来看,雄性家蚕的数据在各生长阶段均更显著,但对雌性家蚕的影响较小.结论:本项研究为火麻仁油的合理开发提供新视角,且火麻仁油对不同性别家蚕的不同影响产生的原因有待于进一步研究.

  15. Unexpected effects of sublethal doses of insecticide on the peripheral olfactory response and sexual behavior in a pest insect.

    Science.gov (United States)

    Lalouette, Lisa; Pottier, Marie-Anne; Wycke, Marie-Anne; Boitard, Constance; Bozzolan, Françoise; Maria, Annick; Demondion, Elodie; Chertemps, Thomas; Lucas, Philippe; Renault, David; Maibeche, Martine; Siaussat, David

    2016-02-01

    Pesticides have long been used as the main solution to limit agricultural pests, but their widespread use resulted in chronic or diffuse environmental pollutions, development of insect resistances, and biodiversity reduction. The effects of low residual doses of these chemical products on organisms that affect both targeted species (crop pests) but also beneficial insects became a major concern, particularly because low doses of pesticides can induce unexpected positive--also called hermetic--effects on insects, leading to surges in pest population growth at greater rate than what would have been observed without pesticide application. The present study aimed to examine the effects of sublethal doses of deltamethrin, one of the most used synthetic pyrethroids, known to present a residual activity and persistence in the environment, on the peripheral olfactory system and sexual behavior of a major pest insect, the cotton leafworm Spodoptera littoralis. We highlighted here a hormetic effect of sublethal dose of deltamethrin on the male responses to sex pheromone, without any modification of their response to host-plant odorants. We also identified several antennal actors potentially involved in this hormetic effect and in the antennal detoxification or antennal stress response of/to deltamethrin exposure.

  16. Radiation-induced bystander effects and adaptive responses--the Yin and Yang of low dose radiobiology?

    Energy Technology Data Exchange (ETDEWEB)

    Mothersill, Carmel [Medical Physics and Applied Radiation Sciences Unit, McMaster University, Hamilton, Ont., L8S 4K1 (Canada)]. E-mail: mothers@mcmaster.ca; Seymour, Colin [Medical Physics and Applied Radiation Sciences Unit, McMaster University, Hamilton, Ont., L8S 4K1 (Canada)]. E-mail: seymouc@mcmaster.ca

    2004-12-02

    Our current knowledge of the mechanisms underlying the induction of bystander effects by low doses of high or low LET ionizing radiation is reviewed. The question of what actually constitutes a protective effect is discussed in the context of adaptive (often referred to as hormetic or protective) responses. Finally the review considers critically, how bystander effects may be related to observed adaptive responses or other seemingly protective effects of low doses exposures. Bystander effects induce responses at the tissue level, which are similar to generalized stress responses. Most of the work involving low LET radiation exposure discussed in the existing literature measures a death response. Since many cell populations carry damaged cells without being exposed to radiation (so-called 'background damage'), it is possible that low doses exposures cause removal of cells carrying potentially problematic lesions, prior to exposure to radiation. This mechanism could lead to the production of 'U-shaped' or hormetic dose-response curves. The level of adverse, adaptive or apparently beneficial response will be related to the background damage carried by the original cell population, the level of organization at which damage or harm are scored and the precise definition of 'harm'. This model may be important when attempting to predict the consequences of mixed exposures involving low doses of radiation and other environmental stressors.

  17. Effects of gamma radiation in tomato seeds

    Energy Technology Data Exchange (ETDEWEB)

    Wiendl, Toni A.; Wiendl, Fritz W.; Franco, Suely S.H.; Franco, Jose G.; Althur, Valter, E-mail: tawiendl@hotmail.com, E-mail: gilmita@uol.com.br [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil); Arthur, Paula B., E-mail: arthur@cena.usp.br [Centro de Energia Nuclear na Agricultura (CENA/USP), Piracicaba, SP (Brazil)

    2013-07-01

    Tomato dry seeds of the hybrid 'Gladiador' F1 were exposed to low doses of gamma radiation from Co-60 source at 0,509 kGy tax rate in order to study stimulation effects of radiation on germination and plant growth. Eight treatments radiation doses were applied as follows: 0 (control); 2,5; 5,0; 7,5; 10,0; 12,5; 15,0; 20,0 Gy. Seed germination as well as green fruits number, harvested fruit number, fruit weight and total production were assessed to identify occurrence of stimulation. Tomato seeds and plants were handled as for usual tomato production in Brazil. Low doses of gamma radiation treatment in the seeds stimulate germination and substantially increase fruit number and total production up to 86% at 10 Gy dose. There are evidences that the use of low doses of gamma radiation can stimulate germination and plant production thus, showing hormetic effects. (author)

  18. Toxicological effects involved in risk assessment of rare earth lanthanum on roots of Viciafaba L.seedlings

    Institute of Scientific and Technical Information of China (English)

    Chengrun Wang; Mei He; Wen Shi; Jessie Wong; Tao Cheng; Xiaorong Wang; Lingling Hu; Fenfen Chen

    2011-01-01

    Combined chemical analyses and biological measurements were utilized to investigate potential toxicological effects and possible mechanisms involved in risk assessment of rare earth elements (REEs) on Viciafaba L.seedlings,which were hydroponically cultivated and exposed to various concentrations of lanthanum (La) for 15 days.The results showed that La contents in both the solution and roots increased with the increase of extraneous La,contributing to hormetic dose responses of superoxide dismutase (SOD),catalase (CAT),guaiacol peroxidase (GPX),ascorbate peroxidase (APX) and endoprotease (EP) isozymes activities,and HSP 70 production enhanced at low doses but suppressed at higher doses of La.These physiological responses constituted antioxidant and detoxification systems against La-induced oxidative stress.The elevated La levels also contributed to oxidatively modified proteins,which were most responsible for subsequent cell death and growth retardation of the roots.By combination of hormetic and traditional threshold dose levels,the threshold dose range was deduced to be 108-195 μg La/g dry weight in the roots,corresponding to 0.90-3.12 mg/L of soluble La in the culture solution.It suggests that persistent applications of REEs may lead to potential ecological risk in the environment.

  19. Effects of moderate energy restriction on physical performance and substrate utilization in non-obese men

    NARCIS (Netherlands)

    Velthuis-te Wierik, E.J.M.; Hoogzaad, L.V.; Berg, H. van den; Schaafsma, G.

    1994-01-01

    Energy restriction (ER) has shown to be an effective 'anti-aging' factor in rodents, resulting in an increased life span and preventing or delaying the occurrence of many age-related diseases in rodents. As a part of a feasibility study on the potential application of ER in humans, we studied the ef

  20. Low Dose Sex Hormone Supplementary Therapy in Women's Anti-aging Treatment%小剂量性激素补充治疗在女性抗衰老中的研究进展

    Institute of Scientific and Technical Information of China (English)

    王泉梅; 周坚红; 周金玲

    2014-01-01

    众所周知,性激素补充治疗能够有效改善妇女的绝经相关症状,而小剂量性激素补充治疗较常规剂量性激素补充治疗的不良反应更少。故本文就小剂量性激素补充治疗在女性心血管衰老、外阴阴道萎缩、性功能减退、骨质疏松、认知功能减退等方面的改善作用及其不良反应进行综述。%As we all know sex hormone supplementary therapy can effectively improve women's menopause-related symptoms. Besides,low dose sex hormone supplementary therapy has less side effects compared with the conventional dose sex hormone supplementary therapy. This article reviews the effect and side effect of low dose sex hormone supplementary therapy in terms of cardiovascular aging,atrophy of vulva and vagina,sexual dysfunction,osteoporosis and cognitive decline.

  1. Multiple mild heat-shocks decrease the Gompertz component of mortality in Caenorhabditis elegans.

    Science.gov (United States)

    Wu, Deqing; Cypser, James R; Yashin, Anatoli I; Johnson, Thomas E

    2009-09-01

    Exposure to mild heat-stress (heat-shock) can significantly increase the life expectancy of the nematode Caenorhabditis elegans. A single heat-shock early in life extends longevity by 20% or more and affects life-long mortality by decreasing initial mortality only; the rate of increase in subsequent mortality (Gompertz component) is unchanged. Repeated mild heat-shocks throughout life have a larger effect on life span than does a single heat-shock early in life. Here, we ask how multiple heat-shocks affect the mortality trajectory in nematodes and find increases of life expectancy of close to 50% and of maximum longevity as well. We examined mortality using large numbers of animals and found that multiple heat-shocks not only decrease initial mortality, but also slow the Gompertz rate of increase in mortality. Thus, multiple heat-shocks have anti-aging hormetic effects and represent an effective approach for modulating aging.

  2. Effects of 2-year calorie restriction on circulating levels of IGF-1, IGF-binding proteins and cortisol in non-obese men and women: a randomized clinical trial

    Science.gov (United States)

    Young-onset calorie restriction (CR) in rodents decreases serum IGF-1 concentration and increases serum corticosterone levels, which have been hypothesized to play major roles in mediating its anti-cancer and anti-aging effects. However, little is known on the effects of CR on the IGF-1 system and c...

  3. Does aging need its own program, or is the program of development quite sufficient for it? Stationary cell cultures as a tool to search for anti-aging factors.

    Science.gov (United States)

    Khokhlov, Alexander N

    2013-02-01

    According to our conception, the aging process is caused by cell proliferation restriction-induced accumulation of various macromolecular defects (mainly DNA damage) in cells of a mature organism or in a cell population. In the case of cell cultures, the proliferation restriction is related to so-called contact inhibition and to the Hayflick's limit, while in the case of multicellular organisms, it is related to the appearance, in the process of differentiation, of organs and tissues consisting of postmitotic and very slowly dividing cells. It is assumed that the proliferation of intact cells prevents accumulation of various errors in a cell population. However, the continuous propagation of all the cells in a multicellular organism is absolutely incompatible with its normal functioning. Thus, the program of development, when it generates postmitotic or slowly dividing cells, automatically leads also to the onset of the aging process (mortality increase with age). Therefore, any additional special program for aging simply becomes unnecessary. This, however, doesn't reject, for some organisms, the reasonability of programmed death, which makes possible the elimination of harmful, from the species point of view, individuals. It is also very important to emphasize that increase or decrease of an organism's lifespan under the effects of various external factors is not always necessarily related to modification of the aging process, though the experimental results in the field are usually interpreted in just this way. I called the experimental-gerontological models similar to the Hayflick's model "correlative", since they are based on some correlations only and not related necessarily to the gist of the aging phenomenon. So, for the Hayflick's model, it is the relationship between population doubling level and donor age, between population doubling potential and species lifespan, between some cell changes in vivo and in vitro, and so forth. If the rationale of the

  4. A will to youth: the woman's anti-aging elixir.

    Science.gov (United States)

    Smirnova, Michelle Hannah

    2012-10-01

    The logic and cultural myths that buttress the cosmeceutical industry construct the older woman as a victim of old age, part of an "at-risk" population who must monitor, treat and prevent any markers of old age. A content and discourse analysis of 124 advertisements from the US More magazine between 1998 and 2008, revealed three major themes working together to produce this civic duty: (1) the inclusion of scientific and medical authorities in order to define the cosmeceutical as a 'drug' curing a disease, (2) descriptions of the similarities (and differences) between the abilities of cosmeceuticals and cosmetic surgery to restore one's youth, and (3) the logic equating youth with beauty, femininity and power and older age with the absence of these qualities. Together these intersecting logics produce the "will to youth"-the imperative of the aging woman to promote her youthful appearance by any and all available means. Further, by using images and references to fantasies and traditional fairytales, cosmeceutical advertisements both promise and normalize expectations of eternal youth of the aging woman.

  5. Anti-Aging Medicine: Can Consumers Be Better Protected?

    Science.gov (United States)

    Mehlman, Maxwell J.; Binstock, Robert H.; Juengst, Eric T.; Ponsaran, Roselle S.; Whitehouse, Peter J.

    2004-01-01

    The use of interventions claiming to prevent, retard, or reverse aging is proliferating. Some of these interventions can seriously harm older persons and aging baby boomers who consume them. Others that are merely ineffective may divert patients from participating in beneficial regimens and also cause them economic harm. "Free market…

  6. How to Select Anti-Aging Skin Care Products

    Science.gov (United States)

    ... for public Dermatology World Dialogues in Dermatology JAAD Mohs AUC MyDermPath+ Psoriasis Patient education resources Practice Management ... clinical trials and received approval from the U.S. Food and Drug Administration (FDA). Select a product within ...

  7. How to Create an Anti-Aging Skin Care Plan

    Science.gov (United States)

    ... for public Dermatology World Dialogues in Dermatology JAAD Mohs AUC MyDermPath+ Psoriasis Patient education resources Practice Management ... lines and wrinkles, and leathery skin. Eat healthy foods: A healthy diet promotes healthy skin. Make sure ...

  8. Apple Can Act as Anti-Aging on Yeast Cells

    Directory of Open Access Journals (Sweden)

    Vanessa Palermo

    2012-01-01

    Full Text Available In recent years, epidemiological and biochemical studies have shown that eating apples is associated with reduction of occurrence of cancer, degenerative, and cardiovascular diseases. This association is often attributed to the presence of antioxidants such as ascorbic acid (vitamin C and polyphenols. The substances that hinder the presence of free radicals are also able to protect cells from aging. In our laboratory we used yeast, a unicellular eukaryotic organism, to determine in vivo efficacy of entire apples and their components, such as flesh, skin and polyphenolic fraction, to influence aging and oxidative stress. Our results indicate that all the apple components increase lifespan, with the best result given by the whole fruit, indicating a cooperative role of all apple components.

  9. Effect of moderate static electric field on the growth and metabolism of Chlorella vulgaris.

    Science.gov (United States)

    Nezammahalleh, Hassan; Ghanati, Faezeh; Adams, Thomas A; Nosrati, Mohsen; Shojaosadati, Seyed Abbas

    2016-10-01

    An electric field (EF) generator device was fabricated and applied to the treatment of Chlorella vulgaris ISC33 at three distinct concentrations before cultivation. The EF of moderate intensity (2.7kVcm(-1)) has a hormetic effect on algal growth. The highest growth stimulation of 51% was observed after 50min treatment of 0.4gL(-1) algal suspension. The influence of EF on the system was then studied from both theoretical and experimental perspectives. The growth rate increased with treatment time up to a maximum because of improved membrane permeability, and then declined afterwards due to peroxide accumulation in the medium. The contents of chlorophylls, carotenoids, soluble carbohydrates, lipids, and proteins were also measured to understand possible changes on algal metabolism. The EF treatment of algal suspension has no observable effect on the cell metabolism while both algal growth and metabolism was significantly affected by the inoculum size.

  10. 抗衰老保健食品中白藜芦醇和辅酶Q_(10)的含量测定%Determination of Resveratrol and Coenzyme Q_(10) in Anti-aging Health-care Food

    Institute of Scientific and Technical Information of China (English)

    姜伟; 杨小妹; 陆豫; 余勃

    2012-01-01

    建立了同时测定抗衰老保健食品中白藜芦醇和辅酶Q10的含量的HPLC方法。采用Welch materials C18液相色谱柱,甲醇∶异丙醇(55∶45)为流动相,流速设定为1 mL·min^-1,检测波长为PDA定时波长(0.00,306.0)(6.00,306.0)(6.01,275.0)(20.00,275.0),实现了白藜芦醇和辅酶Q10的良好分离分析。白藜芦醇的线性范围为0.52~156.00μg·mL^-1,r=0.9999;辅酶Q10的线性范围为0.49~195.52μg.mL^-1,r=0.9999。平均加标回收率及RSD分别为97.70%(RSD 0.6%)和97.40%(RSD 0.4%)。本方法灵敏度高、选择性好、操作简单,可方便地用于检测抗衰老类保健食品中白藜芦醇和辅酶Q10的含量。%A high performance liquid chromatographic(HPLC) method was established for the simultaneous determination of resveratrol and Coenzyme Q10 in anti-aging health-care food.The determination of resveratrol and Coenzyme Q10 by HPLC was performed with Welch materials ODS C18 and the mobile phase of methanol:isopropanol(55:45) at a flow rate of 1.0 mL·min^-1,and the detection wavelength was conducted at(0.00,306.0),(6.00,306.0),(6.01,275.0),and(20.00,275.0).Results showed that a good separation of resveratrol and Coenzyme Q10 was observed.The calibration curve of resveratrol was linear within the range of 0.52~156.00 μg·mL-1 and r=0.9999.The calibration curve of Coenzyme Q10 was linear within the range of 0.49~195.52 μg·mL^-1 and r=0.9999.The average recovery rate of resveratrol was 97.70% and the RSD was 0.6%.The average recovery rate of Coenzyme Q10 was 97.40% and the RSD was 0.4%.The HPLC method was characterized by high sensitivity,good selectivity,simple operation of determination of resveratrol and Coenzyme Q10 in anti-aging health-care food.

  11. Context- and dose-dependent modulatory effects of naringenin on survival and development of Drosophila melanogaster.

    Science.gov (United States)

    Chattopadhyay, Debarati; Sen, Soumadeep; Chatterjee, Rishita; Roy, Debasish; James, Joel; Thirumurugan, Kavitha

    2016-04-01

    Naringenin, the predominant bioflavonoid found in grapefruit and tomato has diverse bioactive properties that encompass anti-carcinogenic, anti-inflammatory, anti-atherogenic, anti-estrogenic, anti-hyperlipidemic and anti-hyperglycemic characteristics. Naringenin has not been explored for its pro-longevity traits in fruit flies. Therefore, the current study explores its influence on longevity, fecundity, feeding rate, larval development, resistance to starvation stress and body weight in male and female wild-type Drosophila melanogaster Canton-S flies. Flies were fed with normal and high fat diets respectively. The results implied hormetic effects of naringenin on longevity and development in flies. In flies fed with standard and high fat diets, lower concentrations of naringenin (200 and 400 µM) augmented mean lifespan while higher concentrations (600 and 800 µM) were consistently lethal. However, enhanced longevity seen at 400 µM of naringenin was at the expense of reduced fecundity and food intake in flies. Larvae reared on standard diet having 200 µM of naringenin exhibited elevated pupation and emergence as flies. Eclosion time was hastened in larvae reared on standard diet having 200 µM of naringenin. Female flies fed with a standard diet having 200 and 400 µM of naringenin were more resistant to starvation stress. Reduction in body weight was observed in male and female flies fed with a high fat diet supplemented with 200 and 400 µM of naringenin respectively. Collectively, the results elucidated a context- and dose-dependent hormetic efficacy of naringenin that varied with gender, diet and stage of lifecycle in flies.

  12. Molecular stress response pathways as the basis of hormesis

    DEFF Research Database (Denmark)

    Demirovic, Dino; de Toda, Irene Martinez; Rattan, Suresh

    2014-01-01

    There is now a large amount of data available for human beings showing positive hormetic effects of mild stresses from physical, chemical, nutritional and mental sources. However, these data are dispersed in the literature and not always interpreted as hormetic effects, thus restricting their ful...

  13. Hormesis in health and disease

    DEFF Research Database (Denmark)

    There is now a large amount of data available for human beings showing positive hormetic effects of mild stresses from physical, chemical, nutritional and mental sources. However, these data are dispersed in the literature and not always interpreted as hormetic effects, thus restricting their ful...

  14. Synergistic Anti-aging Effect of Two Types of Probiotic Yogurts Containing Agrocybe aegerita Polysaccharides and Hypsizigus marmoreus Polysaccharides on Skin%两种真菌多糖益生菌酸奶协同抗皮肤衰老的研究

    Institute of Scientific and Technical Information of China (English)

    李广富; 陈伟; 李听听; 卢中一

    2016-01-01

    对D-半乳糖致衰老小鼠分别灌胃添加茶新菇多糖或真姬菇多糖的益生菌酸奶,研究两种真菌多糖酸奶对小鼠抗皮肤衰老作用的协同效果.结果显示,皮肤组织经HE染色观察,衰老组小鼠皮肤发生病变,而高剂量组小鼠皮肤修复效果最佳.两种真菌多糖益生菌酸奶均可显著提高小鼠皮肤中SOD活性及含水量,降低MDA含量,提高皮肤和尾腱中HYP含量(p<0.05),且与多糖添加量呈剂量增强效应.与衰老组相比,茶新菇多糖酸奶和真姬菇多糖酸奶高剂量组小鼠皮肤中SOD活力提高了66.31%、14.54%,含水率提高16.49%、8.93%,皮肤和尾腱中羟脯氨酸含量分别提高48.27%、65.59%和73.98%、61.63%,MDA含量降低38.96%、45.64%,皮肤抗衰老指标极显著改善(p<0.01).结论:两种真菌多糖均可与益生菌酸奶在皮肤抗衰老方面发挥显著协同作用,有效修复皮肤损伤.

  15. Drinking pattern of wine and effects on human health: why should we drink moderately and with meals?

    Science.gov (United States)

    Boban, Mladen; Stockley, Creina; Teissedre, Pierre-Louis; Restani, Patrizia; Fradera, Ursula; Stein-Hammer, Claudia; Ruf, Jean-Claude

    2016-07-13

    Conclusions of epidemiological studies examining the effects of alcoholic beverages on human health may be unclear and limited if they do not take into account drinking pattern parameters such as beverage type, regular moderate versus binge drinking and drinking with or without meals. This review considers different aspects of drinking patterns and effects on human health with special attention to wine. We particularly discuss the potential underlying mechanisms for epidemiological evidence that the beneficial effects of wine are more evident if consumed with food. In this context, we address the effects of food on blood alcohol concentration and acetaldehyde production in the gastrointestinal tract, the role of wine components and uric acid in counteracting the detrimental effects of postprandial oxidative stress, as well as wine's antimicrobial properties and its potential to act as a digestive aid. In addition to its biological correlates, drinking patterns with regard to different socio-cultural circumstances in different populations are also considered. In order to avoid confusion and misconceptions in the general population because of the hormetic associations of wine with human health, it is important that all medical and scientific information concerning the effect of wine consumption on human health are evidence-based and communicated in a competent, credible and unbiased manner. In conclusion, we propose several practical recommendations concerning wine consumption and consumer information to minimize the risks of alcohol-related harm and to encourage individual responsibility and a healthy lifestyle.

  16. Preconditioning is hormesis part II: How the conditioning dose mediates protection: Dose optimization within temporal and mechanistic frameworks.

    Science.gov (United States)

    Calabrese, Edward J

    2016-08-01

    In Part I, hormetic doses of a variety of agents stimulated adaptive responses that conditioned and protected cells against the subsequent toxicity resulting from a second, higher dose (called a challenging dose) of the same or different agents. Herein (Part II), the optimal conditioning (hormetic) doses of many agents are documented, cellular mechanisms and temporal profiles are examined from which the conditioning (hormetic) responses are elicited, and the optimal conditioning doses are compared to the levels at which optimal protection occurs in response to the toxic challenge dose. Entry criteria for study evaluation required a conditioning mechanism-induced endpoint response, an hormetic/biphasic dose response for the protective response following the challenging dose, and a mechanistic assessment of how the conditioning dose afforded protection against a toxic challenging dose. The conditioning dose that demonstrated the largest increase in a mechanism-related conditioning (hormetic) response (i.e., prior to administration of the challenging dose) was the same dose that was optimally protective following the challenging dose. Specific receptor antagonists and/or inhibitors of cell signaling pathways which blocked the induction of conditioning (hormetic) effects during the conditioning period abolished the protective effects following the application of a challenge dose, thus identifying a specific and essential component of the hormetic mechanism. Conditioning responses often had sufficient doses to assess the nature of the dose response. In each of the cases these mechanism-based endpoints displayed an hormetic dose response. The present analysis reveals that hormetic biphasic dose responses were associated with both the conditioning process and the protective effects elicited following the challenging dose. Furthermore, based on optimal dosage, temporal relationships and the known mediating actions of receptor-based and/or cell signaling-based mechanisms

  17. Behavioral and metabolic effects of sublethal doses of two insecticides, chlorpyrifos and methomyl, in the Egyptian cotton leafworm, Spodoptera littoralis (Boisduval) (Lepidoptera: Noctuidae).

    Science.gov (United States)

    Dewer, Youssef; Pottier, Marie-Anne; Lalouette, Lisa; Maria, Annick; Dacher, Matthieu; Belzunces, Luc P; Kairo, Guillaume; Renault, David; Maibeche, Martine; Siaussat, David

    2016-02-01

    Insecticides have long been used as the main method in limiting agricultural pests, but their widespread use has resulted in environmental pollution, development of resistances, and biodiversity reduction. The effects of insecticides at low residual doses on both the targeted crop pest species and beneficial insects have become a major concern. In particular, these low doses can induce unexpected positive (hormetic) effects on pest insects, such as surges in population growth exceeding what would have been observed without pesticide application. Methomyl and chlorpyrifos are two insecticides commonly used to control the population levels of the cotton leafworm Spodoptera littoralis, a major pest moth. The aim of the present study was to examine the effects of sublethal doses of these two pesticides, known to present a residual activity and persistence in the environment, on the moth physiology. Using a metabolomic approach, we showed that sublethal doses of methomyl and chlorpyrifos have a systemic effect on the treated insects. We also demonstrated a behavioral disruption of S. littoralis larvae exposed to sublethal doses of methomyl, whereas no effects were observed for the same doses of chlorpyrifos. Interestingly, we highlighted that sublethal doses of both pesticides did not induce a change in acetylcholinesterase activity in head of exposed larvae.

  18. Clinical Evidence of Effects of Lactobacillus plantarum HY7714 on Skin Aging: A Randomized, Double Blind, Placebo-Controlled Study.

    Science.gov (United States)

    Lee, Dong Eun; Huh, Chul-Sung; Ra, Jehyeon; Choi, Il-Dong; Jeong, Ji-Woong; Kim, Sung-Hwan; Ryu, Ja Hyun; Seo, Young Kyoung; Koh, Jae Sook; Lee, Jung-Hee; Sim, Jae-Hun; Ahn, Young-Tae

    2015-12-28

    The beneficial effects of probiotics are now widely reported, although there are only a few studies on their anti-aging effects. We have found that Lactobacillus plantarum HY7714 (HY7714) improves skin hydration and has anti-photoaging effects, and in the present study, we have further evaluated the anti-aging effect of HY7714 via a randomized, double blind, placebo-controlled clinical trial. The trial included 110 volunteers aged 41 and 59 years who have dry skin and wrinkles. Participants took 1 × 10(10) CFU/day of HY7714 (probiotic group) or a placebo (placebo group) for 12 weeks. Skin hydration, wrinkles, skin gloss, and skin elasticity were measured every 4 weeks during the study period. There were significant increases in the skin water content in the face (p < 0.01) and hands (p < 0.05) at week 12 in the probiotic group. Transepidermal water loss decreased significantly in both groups at weeks 4, 8, and 12 (p < 0.001 compared with baseline), and was suppressed to a greater extent in the face and forearm in the probiotic group at week 12. Volunteers in the probiotic group had a significant reduction in wrinkle depth at week 12, and skin gloss was also significantly improved by week 12. Finally, skin elasticity in the probiotic group improved by 13.17% (p < 0.05 vs. controls) after 4 weeks and by 21.73% (p < 0.01 vs. controls) after 12 weeks. These findings are preliminary confirmation of the anti-aging benefit to the skin of L. plantarum HY7714 as a nutricosmetic agent.

  19. Effects of urban land-use on largescale stonerollers in the Mobile River Basin, Birmingham, AL

    Science.gov (United States)

    Iwanowicz, Deborah; Black, M.C.; Blazer, Vicki; Zappia, H.; Bryant, Wade L.

    2016-01-01

    During the spring and fall of 2001 and the spring of 2002 a study was conducted to evaluate the health of the largescale stoneroller (Campostoma oligolepis) populations in streams along an urban land-use gradient. Sites were selected from a pool of naturally similar sub-basins (eco-region, basin size, and geology) of the Mobile River basin (MRB), using an index of urban intensity derived from infrastructure, socioeconomic, and land-use data. This urban land-use gradient (ULUG) is a multimetric indicator of urban intensity, ranging from 0 (background) to 100 (intense urbanization). Campostoma sp. have been used previously as indicators of stream health and are common species found in all sites within the MRB. Endpoints used to determine the effects of urban land-use on the largescale stoneroller included total glutathione, histology, hepatic apoptosis, condition factor and external lesions. Liver glutathione levels were positively associated with increasing urban land-use (r2 = 0.94). Histopathological examination determined that some abnormalities and lesions were correlated with the ULUG and generally increased in prevalence or severity with increasing urbanization. Liver macrophage aggregates were positively correlated to the ULUG. The occurrence of nucleosomal ladders (indicating apoptotic cell death) did not correspond with urban intensity in a linear fashion. Apoptosis, as well as prevalence and severity of a myxozoan parasite, appeared to have a hormetic dose–response relationship. The majority of the biomarkers suggested fish health was compromised in areas where the ULUG ≥ 36.

  20. Hormetic response of cholinesterase from Daphnia magna in chronic exposure to triazophos and chlorpyrifos

    Institute of Scientific and Technical Information of China (English)

    Shaonan Li; Yajun Tan

    2011-01-01

    In vivo activity of cholinesterase (ChE) in Daphnia magna was measured at different time points during 21-day exposure to triazophos and chlorpyrifos ranging from 0.05 to 2.50 μg/L and 0.01 to 2.00 μg/L, respectively.For exposure to triazophos, ChE was induced up to 176.5% at 1.5 μg/L and day 10 when measured by acetylthiocholine (ATCh), whereas it was induced up to 174.2% at 0.5 μg/L and day 10 when measured by butyrylthiocholine (BTCh).For exposure to chlorpyrifos, ChE was induced up to 134.0% and 160.5% when measured by ATCh and BTCh, respectivly, with both maximal inductions detected at 0.l μg/L and day 8.Obvious induction in terms of ChE activity was also detected in daphnia removed from exposures 24 hr after their birth and kept in a recovery culture for 21 days.Results indicated that the enzyme displayed symptoms of hormesis, a characteristic featured by conversion from low-dose stimulation to high-dose inhibition.In spite of that, no promotion in terms of reproduction rate and body size was detected at any tested concentrations regardless of whether the daphnia were collected at end of the 21-day exposure or at end of a 21-day recovery culture.This suggested that induction of ChE caused by anticholinesterases had nothing to do with the prosperity of the daphnia population.

  1. Schisandrin B as a Hormetic Agent for Preventing Age-Related Neurodegenerative Diseases

    OpenAIRE

    Lam, Philip Y.; Kam Ming Ko

    2012-01-01

    Oxidative stress and mitochondrial dysfunction have been implicated in the pathogenesis of neurodegenerative diseases, with the latter preceding the appearance of clinical symptoms. The energy failure resulting from mitochondrial dysfunction further impedes brain function, which demands large amounts of energy. Schisandrin B (Sch B), an active ingredient isolated from Fructus Schisandrae, has been shown to afford generalized tissue protection against oxidative damage in various organs, includ...

  2. Ozone acting on human blood yields a hormetic dose-response relationship

    Directory of Open Access Journals (Sweden)

    Travagli Valter

    2011-05-01

    Full Text Available Abstract The aim of this paper is to analyze why ozone can be medically useful when it dissolves in blood or in other biological fluids. In reviewing a number of clinical studies performed in Peripheral Arterial Diseases (PAD during the last decades, it has been possible to confirm the long-held view that the inverted U-shaped curve, typical of the hormesis concept, is suitable to represent the therapeutic activity exerted by the so-called ozonated autohemotherapy. The quantitative and qualitative aspects of human blood ozonation have been also critically reviewed in regard to the biological, therapeutic and safety of ozone. It is hoped that this gas, although toxic for the pulmonary system during prolonged inhalation, will be soon recognized as a useful agent in oxidative-stress related diseases, joining other medical gases recently thought to be of therapeutic importance. Finally, the elucidation of the mechanisms of action of ozone as well as the obtained results in PAD may encourage clinical scientists to evaluate ozone therapy in vascular diseases in comparison to the current therapies.

  3. Effects of palmitoyl-KVK-L-ascorbic acid on skin wrinkles and pigmentation.

    Science.gov (United States)

    Kim, Hyeong Mi; An, Hyo Sun; Bae, Jung-Soo; Kim, Jung Yun; Choi, Chi Ho; Kim, Ju Yeon; Lim, Joo Hyuck; Choi, Joon-Hun; Song, Hyunnam; Moon, Sung Ho; Park, Young Jun; Chang, Shin-Jae; Choi, Sun Young

    2017-03-16

    Wrinkle formation and abnormal pigmentation are major clinical alterations associated with skin aging. As the aim of our study was to investigate the effects of palmitoyl-KVK-L-ascorbic acid on skin aging, the anti-wrinkle and depigmentation effects of palmitoyl-KVK-L-ascorbic acid were evaluated by measuring collagen expression in dermal fibroblast cells and inhibition of melanogenesis in B16F1 cells, respectively. The anti-aging effect of palmitoyl-KVK-L-ascorbic acid cream was also evaluated against a placebo cream in a clinical trial. Our results confirmed that the expression of type Ι collagen in dermal fibroblast cells treated with palmitoyl-KVK-L-ascorbic acid (0.1-4 μg/mL) increased in a dose-dependent manner. In B16F1 cells, treatment with 20 μg/mL palmitoyl-KVK-L-ascorbic acid reduced the melanin content by approximately 20% compared to alpha-melanocyte stimulating hormone treatment. In the clinical trial, application of palmitoyl-KVK-L-ascorbic acid cream led to an improvement in skin roughness and lightness in 12 and 8 weeks, respectively. Our data show that palmitoyl-KVK-L-ascorbic acid is an effective anti-aging agent that reduces wrinkles and abnormal skin pigmentation.

  4. Clinical Study on Anti-aging Action of Herbal Cake-partition Moxibustion

    Institute of Scientific and Technical Information of China (English)

    赵粹英; 张英英; 王虹英; 杨玲; 陈汉平; 居贤水; 吴恒举; 丁菊英; 施征; LI Sa; 洪娴

    2009-01-01

    Objective:To investigate the clinical efficacy and immunological mechanism of herbal cake-partitioned moxibustion for aging process.Method:The herbal cake-partitioned moxibustion was adopted for 223 cases to observe the aging scores before and after the treatment.Apart from that,the T-lymphocyte subsets and changes of IL-2 and 3-EP were also detected.Results:After treatment,the aging scores of 223 cases were all substantially reduced,along with an improvement of clinical symptoms,a strengthened cellular immune function,and an increase of total T-lymphocyte count.In addition,the CD4+/CD8+ ratio Was restored normal,the synthesis or secretion of IL-2 was increased and the β-EP(as the neurotransmitter to modulate immune function)was substantially improved.Conclusion:The aging process is closely associated with the immune function.Moxibustion Can modulate abnormal immune function and stabilize homeostasis and thus delay the aging process.%目的:探讨隔药饼灸抗衰老的临床疗效和免疫学机理.方法:采用隔药饼灸治疗223例老年人,观察隔药饼灸治疗前及治疗1疗程结束后衰老见症积分值,并检测血T淋巴细胞亚群、IL-2和β-内啡肽的变化.结果:223例老人经灸治后,衰老积分明显下降,在临床症状改善同时细胞免疫功能增强,T淋巴细胞总数增加,CD4+/CDs+比值恢复正常,IL-2合成分泌增加.β-EP作为免疫调节的神经介质,灸治后明显提高.结论:衰老过程与免疫功能密切相关.艾灸能纠正异常免疫状态、稳定机体内环境达到延缓衰老 目的.

  5. Anti-aging : Una relectura contemporània de Dorian Gray

    OpenAIRE

    Braut Canes, Montse

    2013-01-01

    Partint de l'estudi del llibre El retrat de Dorian Gray d'Oscar Wilde s'analitzarà, des del punt de vista de la cultura actual, una societat que s'aferra a la joventut i utilitza tots els mètodes tecnològics per defugir de la vellesa. Partiendo del estudio del libro El retrato de Dorian Gray de Oscar Wilde se analizará, desde el punto de vista de la cultura actual, una sociedad que se aferra a la juventud y utiliza todos los métodos tecnológicos para huir de la vejez. Bachelor thesis fo...

  6. Development of Multifunctional Anti Aging Military Raincoat Fabric by Using Fitting Technique

    Directory of Open Access Journals (Sweden)

    Chen Chun Xia

    2016-01-01

    Full Text Available Use Woodland camouflage paint Oxford fabric as base cloth, first the light pressure processing was used on the fabric, and then use polyurethane which has high water vapor permeability performance and other various additives as face glue and Bottom glue, the fabric was compounded a thin macromolecule hydrophilic polymer PU film, the fabric was water allocation processed after stripping, At last the fabric will have high performance of the windproof, waterproof and moisture permeability. After testing, the performance index of the fabric are: the smoothness appearance of fabrics after cleaning≥grade4.0; hydrostatic pressure≥10000mmH2O, after 20 times washing≥5000mmH2O, The surface water repellency level≥grade 4.0,after 20 times washing≥grade3.0, water vapor permeability≥3500g/(m2·24h; Electrostatic charge density≥2.5uc/m2; humid air accelerated aging grade≥4.0.Test data shows that the designed functional fabric meet the technology requirement of the standards, meet the demand of the customer’s use requirements.

  7. Development of Multifunctional Anti Aging Military Raincoat Fabric by Using Fitting Technique

    OpenAIRE

    Chen Chun Xia

    2016-01-01

    Use Woodland camouflage paint Oxford fabric as base cloth, first the light pressure processing was used on the fabric, and then use polyurethane which has high water vapor permeability performance and other various additives as face glue and Bottom glue, the fabric was compounded a thin macromolecule hydrophilic polymer PU film, the fabric was water allocation processed after stripping, At last the fabric will have high performance of the windproof, waterproof and moisture permeability. After...

  8. Attenuation of Replication Stress–Induced Premature Cellular Senescence to Assess Anti-Aging Modalities

    OpenAIRE

    Zhao, Hong; Darzynkiewicz, Zbigniew

    2014-01-01

    Described is an in vitro model of premature senescence in pulmonary adenocarcinoma A549 cells induced by persistent DNA replication stress in response to treatment with the DNA damaging drug mitoxantrone (Mxt). The degree of cellular senescence, based on characteristic changes in cell morphology, is measured by laser scanning cytometry. Specifically, the flattening of cells grown on slides (considered the hallmark of cellular senescence) is measured as the decline in local intensity of DNA-as...

  9. Circulating endothelial progenitor cells: a new approach to anti-aging medicine?

    Directory of Open Access Journals (Sweden)

    Patel Amit N

    2009-12-01

    Full Text Available Abstract Endothelial dysfunction is associated with major causes of morbidity and mortality, as well as numerous age-related conditions. The possibility of preserving or even rejuvenating endothelial function offers a potent means of preventing/treating some of the most fearful aspects of aging such as loss of mental, cardiovascular, and sexual function. Endothelial precursor cells (EPC provide a continual source of replenishment for damaged or senescent blood vessels. In this review we discuss the biological relevance of circulating EPC in a variety of pathologies in order to build the case that these cells act as an endogenous mechanism of regeneration. Factors controlling EPC mobilization, migration, and function, as well as therapeutic interventions based on mobilization of EPC will be reviewed. We conclude by discussing several clinically-relevant approaches to EPC mobilization and provide preliminary data on a food supplement, Stem-Kine, which enhanced EPC mobilization in human subjects.

  10. 天然抗衰老成分%Natural anti-aging compositions in cosmetics

    Institute of Scientific and Technical Information of China (English)

    李季萍

    2008-01-01

    阳光、环境污染、烟尘和生活压力等会导致皮肤细胞氧化,产生自由基,对肌肤造成干噪、斑点、皱纹、粗糙和松弛等老化伤害。为应对这些对娇嫩肌肤的侵害,人们相继开发出维生素C、维生素E、银杏叶、绿茶以及富含维生素的各种水果,应用于护肤品中。葡萄在被证实具有抗氧化功效之后便成为人们关注的又一个焦点。

  11. Sublethal Effects of Cyantraniliprole and Imidacloprid on Feeding Behavior and Life Table Parameters of Myzus persicae (Hemiptera: Aphididae).

    Science.gov (United States)

    Zeng, Xianyi; He, Yingqin; Wu, Jiaxing; Tang, Yuanman; Gu, Jitao; Ding, Wei; Zhang, Yongqiang

    2016-08-01

    The green peach aphid, Myzus persicae (Sulzer) (Hemiptera: Aphididae), is an agricultural pest that seriously infests many crops worldwide. This study used electrical penetration graphs (EPGs) and life table parameters to estimate the sublethal effects of cyantraniliprole and imidacloprid on the feeding behavior and hormesis of M. persicae The sublethal concentrations (LC30) of cyantraniliprole and imidacloprid against adult M. persicae were 4.933 and 0.541 mg L(-1), respectively. The feeding data obtained from EPG analysis indicated that the count probes and number of short probes (imidacloprid-treated plants. In addition, the phloem-feeding behavior of M persicae was significantly impaired on fed tobacco plants treated with cyantraniliprole and imidacloprid at LC30 Analysis of life table parameters indicated that the growth and reproduction of F1 generation aphids were significantly affected when initial adults were exposed to LC30 of cyantraniliprole and imidacloprid. The nymphal period, female longevity, total preoviposition period, and mean generation time were significantly prolonged when initial adults were exposed to LC30 of imidacloprid. By comparison, these parameters were prolonged but not significantly in the cyantraniliprole treatment. The fecundity and gross reproductive rate were significantly increased in the treated groups. Similarly, the net reproductive rate was greater in the treated group than the control group. Our results indicate that treatment with LC30 of imidacloprid and cyantraniliprole would lead to a hormetic response of M. persicae, with higher likelihood of occurrence when initial adults were exposed to LC30 of cyantraniliprole.

  12. Resveratrol Based Oral Nutritional Supplement Produces Long-Term Beneficial Effects on Structure and Visual Function in Human Patients

    Directory of Open Access Journals (Sweden)

    Stuart Richer

    2014-10-01

    Full Text Available Background: Longevinex® (L/RV is a low dose hormetic over-the-counter (OTC oral resveratrol (RV based matrix of red wine solids, vitamin D3 and inositol hexaphosphate (IP6 with established bioavailability, safety, and short-term efficacy against the earliest signs of human atherosclerosis, murine cardiac reperfusion injury, clinical retinal neovascularization, and stem cell survival. We previously reported our short-term findings for dry and wet age-related macular degeneration (AMD patients. Today we report long term (two to three year clinical efficacy. Methods: We treated three patients including a patient with an AMD treatment resistant variant (polypoidal retinal vasculature disease. We evaluated two clinical measures of ocular structure (fundus autofluorescent imaging and spectral domain optical coherence extended depth choroidal imaging and qualitatively appraised changes in macular pigment volume. We further evaluated three clinical measures of visual function (Snellen visual acuity, contrast sensitivity, and glare recovery to a cone photo-stress stimulus. Results: We observed broad bilateral improvements in ocular structure and function over a long time period, opposite to what might be expected due to aging and the natural progression of the patient’s pathophysiology. No side effects were observed. Conclusions: These three cases demonstrate that application of epigenetics has long-term efficacy against AMD retinal disease, when the retinal specialist has exhausted other therapeutic modalities.

  13. Concentration-Dependent Effects of Rhodiola Rosea on Long-Term Survival and Stress Resistance of Yeast Saccharomyces Cerevisiae: The Involvement of YAP 1 and MSN2/4 Regulatory Proteins.

    Science.gov (United States)

    Bayliak, Maria M; Burdyliuk, Nadia I; Izers'ka, Lilia I; Lushchak, Volodymyr I

    2014-01-01

    Concentration-dependent effects of aqueous extract from R. rosea root on long-term survival and stress resistance of budding yeast Saccharomyces cerevisiae were studied. At low concentrations, R. rosea aqueous extract extended yeast chronological lifespan, enhanced oxidative stress resistance of stationary-phase cells and resistance to number stressors in exponentially growing cultures. At high concentrations, R. rosea extract sensitized yeast cells to stresses and shortened yeast lifespan. These biphasic concentration-responses describe a common hormetic phenomenon characterized by a low-dose stimulation and a high-dose inhibition. Yeast pretreatment with low doses of R. rosea extract enhanced yeast survival and prevented protein oxidation under H2O2-induced oxidative stress. Positive effect of R. rosea extract on yeast survival under heat shock exposure was not accompanied with changes in antioxidant enzyme activities and levels of oxidized proteins. The deficiency in transcriptional regulators, Msn2/Msn4 and Yap1, abolished the positive effect of low doses of R. rosea extract on yeast viability under stress challenges. Potential involvement of Msn2/Msn4 and Yap1 regulatory proteins in realization of R. rosea beneficial effects is discussed.

  14. Vascular dysfunction in aging: potential effects of resveratrol, an anti-inflammatory phytoestrogen.

    Science.gov (United States)

    Labinskyy, Nazar; Csiszar, Anna; Veress, Gabor; Stef, Gyorgyi; Pacher, Pal; Oroszi, Gabor; Wu, Joseph; Ungvari, Zoltan

    2006-01-01

    Epidemiological studies demonstrated that even in the absence of other risk factors (e.g. diabetes, hypertension, hyperhomocysteinemia, hypercholesterolemia), advanced age itself significantly increases cardiovascular morbidity by enhancing vascular oxidative stress and inflammation. Because the population in the Western world is rapidly aging, there is a substantial need for pharmacological interventions that delay the functional decline of the cardiovascular system. Resveratrol is an atoxic phytoestrogen found in more than 70 plants including grapevine and berries. Recent data suggest that nutritional intake of resveratrol and other polyphenol compounds may contribute to the "French paradox", the unexpectedly low cardiovascular morbidity in the Mediterranean population. There is increasing evidence that resveratrol exerts multifaceted anti-oxidant and/or anti-inflammatory effects in various disease models. Importantly, resveratrol was reported to slow aging and increase lifespan in simple organisms and has been suggested as a potential calorie restriction mimetic. Resveratrol has also been reported to activate NAD-dependent histone deacetylases (sirtuins), which may contribute to its anti-aging effects. This review focuses on the role of oxidative stress and inflammation in cardiovascular dysfunction in aging, and on emerging anti-aging therapeutic strategies offered by resveratrol and other polyphenol compounds.

  15. Effect of Selenium Supplementation on Redox Status of the Aortic Wall in Young Spontaneously Hypertensive Rats.

    Science.gov (United States)

    Ruseva, Boryana; Atanasova, Milena; Tsvetkova, Reni; Betova, Tatyana; Mollova, Margarita; Alexandrova, Margarita; Laleva, Pavlina; Dimitrova, Aneliya

    2015-01-01

    Selenium (Se) is an exogenous antioxidant that performs its function via the expression of selenoproteins. The aim of this study was to explore the effect of varying Se intake on the redox status of the aortic wall in young spontaneously hypertensive rats (SHR). Sixteen male Wistar Kyoto (WKY) rats and nineteen male SHR, 16-week-old, were tested after being given diets with different Se content for eight weeks. They were divided into 4 groups: control groups of WKY NSe and SHR NSe on an adequate Se diet and groups of WKY HSe and SHR HSe that received Se supplementation. The Se nutritional status was assessed by measuring whole blood glutathione peroxidase-1 (GPx-1) activity. Serum concentration of lipid hydroperoxides and serum level of antibodies against advanced glycation end products (anti-AGEs abs) were determined. Expression of GPx-1 and endothelial nitric oxide synthase (eNOS) were examined in aortic wall. Se supplementation significantly increased GPx-1 activity of whole blood and in the aortas of WKY and SHR. Decreased lipid peroxidation level, eNOS-3 expression in the aortic wall, and serum level of anti-AGEs abs were found in SHR HSe compared with SHR NSe. In conclusion, Se supplementation improved the redox status of the aortic wall in young SHR.

  16. Effect of Selenium Supplementation on Redox Status of the Aortic Wall in Young Spontaneously Hypertensive Rats

    Directory of Open Access Journals (Sweden)

    Boryana Ruseva

    2015-01-01

    Full Text Available Selenium (Se is an exogenous antioxidant that performs its function via the expression of selenoproteins. The aim of this study was to explore the effect of varying Se intake on the redox status of the aortic wall in young spontaneously hypertensive rats (SHR. Sixteen male Wistar Kyoto (WKY rats and nineteen male SHR, 16-week-old, were tested after being given diets with different Se content for eight weeks. They were divided into 4 groups: control groups of WKY NSe and SHR NSe on an adequate Se diet and groups of WKY HSe and SHR HSe that received Se supplementation. The Se nutritional status was assessed by measuring whole blood glutathione peroxidase-1 (GPx-1 activity. Serum concentration of lipid hydroperoxides and serum level of antibodies against advanced glycation end products (anti-AGEs abs were determined. Expression of GPx-1 and endothelial nitric oxide synthase (eNOS were examined in aortic wall. Se supplementation significantly increased GPx-1 activity of whole blood and in the aortas of WKY and SHR. Decreased lipid peroxidation level, eNOS-3 expression in the aortic wall, and serum level of anti-AGEs abs were found in SHR HSe compared with SHR NSe. In conclusion, Se supplementation improved the redox status of the aortic wall in young SHR.

  17. Melatonin Anticancer Effects: Review

    Directory of Open Access Journals (Sweden)

    Luigi Di Bella

    2013-01-01

    Full Text Available Melatonin (N-acetyl-5-methoxytryptamine, MLT, the main hormone produced by the pineal gland, not only regulates circadian rhythm, but also has antioxidant, anti-ageing and immunomodulatory properties. MLT plays an important role in blood composition, medullary dynamics, platelet genesis, vessel endothelia, and in platelet aggregation, leukocyte formula regulation and hemoglobin synthesis. Its significant atoxic, apoptotic, oncostatic, angiogenetic, differentiating and antiproliferative properties against all solid and liquid tumors have also been documented. Thanks, in fact, to its considerable functional versatility, MLT can exert both direct and indirect anticancer effects in factorial synergy with other differentiating, antiproliferative, immunomodulating and trophic molecules that form part of the anticancer treatment formulated by Luigi Di Bella (Di Bella Method, DBM: somatostatin, retinoids, ascorbic acid, vitamin D3, prolactin inhibitors, chondroitin-sulfate. The interaction between MLT and the DBM molecules counters the multiple processes that characterize the neoplastic phenotype (induction, promotion, progression and/or dissemination, tumoral mutation. All these particular characteristics suggest the use of MLT in oncological diseases.

  18. Effects of radionuclide contamination on leaf litter decomposition in the Chernobyl exclusion zone.

    Science.gov (United States)

    Bonzom, Jean-Marc; Hättenschwiler, Stephan; Lecomte-Pradines, Catherine; Chauvet, Eric; Gaschak, Sergey; Beaugelin-Seiller, Karine; Della-Vedova, Claire; Dubourg, Nicolas; Maksimenko, Andrey; Garnier-Laplace, Jacqueline; Adam-Guillermin, Christelle

    2016-08-15

    The effects of radioactive contamination on ecosystem processes such as litter decomposition remain largely unknown. Because radionuclides accumulated in soil and plant biomass can be harmful for organisms, the functioning of ecosystems may be altered by radioactive contamination. Here, we tested the hypothesis that decomposition is impaired by increasing levels of radioactivity in the environment by exposing uncontaminated leaf litter from silver birch and black alder at (i) eleven distant forest sites differing in ambient radiation levels (0.22-15μGyh(-1)) and (ii) along a short distance gradient of radioactive contamination (1.2-29μGyh(-1)) within a single forest in the Chernobyl exclusion zone. In addition to measuring ambient external dose rates, we estimated the average total dose rates (ATDRs) absorbed by decomposers for an accurate estimate of dose-induced ecological consequences of radioactive pollution. Taking into account potential confounding factors (soil pH, moisture, texture, and organic carbon content), the results from the eleven distant forest sites, and from the single forest, showed increased litter mass loss with increasing ATDRs from 0.3 to 150μGyh(-1). This unexpected result may be due to (i) overcompensation of decomposer organisms exposed to radionuclides leading to a higher decomposer abundance (hormetic effect), and/or (ii) from preferred feeding by decomposers on the uncontaminated leaf litter used for our experiment compared to locally produced, contaminated leaf litter. Our data indicate that radio-contamination of forest ecosystems over more than two decades does not necessarily have detrimental effects on organic matter decay. However, further studies are needed to unravel the underlying mechanisms of the results reported here, in order to draw firmer conclusions on how radio-contamination affects decomposition and associated ecosystem processes.

  19. Tungsten effects on phosphate-dependent biochemical pathways are species and liver cell line dependent.

    Science.gov (United States)

    Johnson, David R; Ang, Chooyaw; Bednar, Anthony J; Inouye, Laura S

    2010-08-01

    Tungsten, in the form of tungstate, polymerizes with phosphate, and as extensive polymerization occurs, cellular phosphorylation and dephosphorylation reactions may be disrupted, resulting in negative effects on cellular functions. A series of studies were conducted to evaluate the effect of tungsten on several phosphate-dependent intracellular functions, including energy cycling (ATP), regulation of enzyme activity (cytosolic protein tyrosine kinase [cytPTK] and tyrosine phosphatase), and intracellular secondary messengers (cyclic adenosine monophosphate [cAMP]). Rat noncancerous hepatocyte (Clone-9), rat cancerous hepatocyte (H4IIE), and human cancerous hepatocyte (HepG2) cells were exposed to 1-1000 mg/l tungsten (in the form of sodium tungstate) for 24 h, lysed, and analyzed for the above biochemical parameters. Cellular ATP levels were not significantly affected in any cell line. After 4 h, tungsten significantly decreased cytPTK activity in Clone-9 cells at >or= 18 mg/l, had no effect in H4IIE cells, and significantly increased cytPTK activity by 70% in HepG2 cells at >or= 2 mg/l. CytPTK displayed a slight hormetic response to tungsten after 24-h exposure yet returned to normal after 48-h exposure. Tungsten significantly increased cAMP by over 60% in Clone-9 cells at >or= 100 mg/l, significantly increased cAMP in H4IIE cells at only 100 mg/l, and significantly increased cAMP in HepG2 cells between 1-100 mg/l but at much more modest levels (8-20%). In conclusion, these data indicate that tungsten produces complex results that must be carefully interpreted in the context of their respective animal models, as well as the phenotype of the cell lines (i.e., normal vs. cancerous).

  20. Therapeutic effects of amla in medicine and dentistry: A review

    Directory of Open Access Journals (Sweden)

    Harpreet Singh Grover

    2015-01-01

    Full Text Available Emblica officinalis (Amla is widely used in the Indian system of medicine and believed to increase defense against diseases. Amla is called amalaki in Sanskrit. It is one of the oldest oriental medicines mentioned in Ayurveda as potential remedy for assorted ailments. A wide range of phytochemical components present in amla including alkaloids, tannins, and flavonoids have been shown to procure useful biological activities. It is an ingredient of many Ayurvedic medicines and tonics as it removes excessive salivation and internal body heat. Research has been done with amla evaluating its role as an antioxidant. Amla is useful in ulcer prevention, for diabetic patients, and for memory effects. Amla Tonic has a hematinic and lipalytic function useful in scurvy, prevents indigestion, and controls acidity as well as it is a natural source of anti-aging.

  1. Dose-dependent effects of R-sulforaphane isothiocyanate on the biology of human mesenchymal stem cells, at dietary amounts, it promotes cell proliferation and reduces senescence and apoptosis, while at anti-cancer drug doses, it has a cytotoxic effect.

    Science.gov (United States)

    Zanichelli, Fulvia; Capasso, Stefania; Cipollaro, Marilena; Pagnotta, Eleonora; Cartenì, Maria; Casale, Fiorina; Iori, Renato; Galderisi, Umberto

    2012-04-01

    Brassica vegetables are attracting a great deal of attention as healthy foods because of the fact that they contain substantial amounts of secondary metabolite glucosinolates that are converted into isothiocyanates, such as sulforaphane [(-)1-isothiocyanato-4R-(methylsulfinyl)-butane] (R-SFN), through the actions of chopping or chewing the vegetables. Several studies have analyzed the biological and molecular mechanisms of the anti-cancer activity of synthetic R,S-sulforaphane, which is thought to be a result of its antioxidant properties and its ability to inhibit histone deacetylase enzymes (HDAC). Few studies have addressed the possible antioxidant effects of R-SFN, which could protect cells from the free radical damage that strongly contribute to aging. Moreover, little is known about the effect of R-SFN on stem cells whose longevity is implicated in human aging. We evaluated the effects of R-SFN on the biology on human mesenchymal stem cells (MSCs), which, in addition to their ability to differentiate into mesenchymal tissues, support hematopoiesis, and contribute to the homeostatic maintenance of many organs and tissues. Our investigation found evidence that low doses of R-SFN promote MSCs proliferation and protect them from apoptosis and senescence, while higher doses have a cytotoxic effect, leading to the induction of cell cycle arrest, programmed cell death and senescence. The beneficial effects of R-SFN may be ascribed to its antioxidant properties, which were observed when MSC cultures were incubated with low doses of R-SFN. Its cytotoxic effects, which were observed after treating MSCs with high doses of R-SFN, could be attributed to its HDAC inhibitory activity. In summary, we found that R-SFN, like many other dietary supplements, exhibits a hormetic behavior; it is able to induce biologically opposite effects at different doses.

  2. Effects of physical activity on systemic oxidative/DNA status in breast cancer survivors

    Science.gov (United States)

    Tomasello, Barbara; Malfa, Giuseppe Antonio; Strazzanti, Angela; Gangi, Santi; Di Giacomo, Claudia; Basile, Francesco; Renis, Marcella

    2017-01-01

    Physical activity offers a paradoxical hormetic effect and a health benefit to cancer survivors; however, the biochemical mechanisms have not been entirely elucidated. Despite the well-documented evidence implicating oxidative stress in breast cancer, the association between health benefits and redox status has not been investigated in survivors who participate in dragon boating. The present study investigated the plasmatic systemic oxidative status (SOS) in breast cancer survivors involved in two distinct physical training exercises. A total of 75 breast cancer survivors were allocated to one of three groups: Control (resting), dragon boat racing and walking group; the latter is a type of aerobic conditioning exercise often advised to cancer patients. Various biochemical oxidative stress markers were examined, including oxidant status (hydroperoxide levels, lipid oxidation) and antioxidant status (enzymatic activities of superoxide dismutase and glutathione peroxidase, reduced glutathione levels and antioxidant capability). In addition, the individual DNA fragmentation and DNA repair capability of nucleotide excision repair (NER) systems were examined by comet assays. According to the results, all patients exhibited high levels of oxidative stress. Physical activity maintained this oxidative stress condition but simultaneously had a positive influence on the antioxidant component of the SOS, particularly in the dragon boat racing group. DNA fragmentation, according to the levels of single- and double-strand breaks, were within the normal range in the two survivor groups that were involved in training activities. Radiation-induced damage was not completely recognised or repaired by NER systems in any of the patients, probably leading to radiosensitivity and/or susceptibility of patients to cancer. These findings suggest that physical activity, particularly dragon boat racing, that modulates SOS and DNA repair capability could represent a strategy for enhancing the

  3. Effect of quercetin on chronic enhancement of spatial learning and memory of mice

    Institute of Scientific and Technical Information of China (English)

    LIU Jiancai; YU Huqing; NING Xinbao

    2006-01-01

    In this study we evaluated the effect of quercetin on D-galactose-induced aged mice using the Morris water maze (MWM) test. Based on the free radical theory of aging, experiments were performed to study the possible biochemical mechanisms of glutathione (GSH) level and hydroxyl radical (OH-) in the hippocampus and cerebral cortex and the brain tissue enzyme activity of the mice. The results indicated that quercetin can enhance the exploratory behavior, spatial learning and memory of the mice. The effects relate with enhancing the brain functions and inhibiting oxidative stress by quercetin, and relate with increasing the GSH level and decreasing the OH- content. These findings suggest that quercetin can work as a possible natural anti-aging pharmaceutical product.

  4. Thermal stress resistance and aging effects of Panax notoginseng polysaccharides on Caenorhabditis elegans.

    Science.gov (United States)

    Feng, Shiling; Cheng, Haoran; Xu, Zhou; Shen, Shian; Yuan, Ming; Liu, Jing; Ding, Chunbang

    2015-11-01

    Panax notoginseng attract public attention due to their potential biomedical properties and corresponding health benefits. The present study investigated the anti-aging and thermal stress resistance effects of polysaccharides from P. notoginseng on Caenorhabditis elegans. Results showed polysaccharides had little scavenging ability of reactive oxygen species (ROS) in vitro, but significantly extended lifespan of C. elegans, especially the main root polysaccharide (MRP) which prolongs the mean lifespan of wild type worms by 21%. Further study demonstrated that the heat stress resistance effect of polysaccharides on C. elegans might be attributed to the elevation of antioxidant enzyme activities (both superoxide dismutase (SOD) and catalase (CAT)) and the reduction lipid peroxidation of malondialdehyde (MDA) level. Taken together, the results provided a scientific basis for the further exploitation of the mechanism of longer lifespan controlled by P. notoginseng polysaccharides on C. elegans. The P. notoginseng polysaccharides might be considered as a potential source to delay aging.

  5. Altered bioenergetics and developmental effects in striped marsh frog (Limnodynastes peronii) tadpoles exposed to UV treated sewage.

    Science.gov (United States)

    Melvin, Steven D; Lanctôt, Chantal M; van de Merwe, Jason P; Leusch, Frederic D L

    2016-06-01

    Effectively treating domestic wastewater so that it can be safely discharged or reused is critical for maintaining the integrity of freshwater resources, and for protecting the health of animals that rely on these systems. Amphibians are currently facing widespread population declines, so there is a particularly urgent need to investigate exposure scenarios that might result in weakened amphibian populations. Domestic sewage has received little attention as a possible factor that could influence the survival, growth and development, or general health of amphibians. However, wastewater reuse for crop irrigation and other purposes is increasing and holding ponds and constructed wetlands exist at many wastewater treatment facilities, introducing conceivable pathways that could result in the exposure of amphibians to treated wastewater. We exposed developing striped marsh frog (Limnodynastes peronii) tadpoles, to control water and 12.5, 25, 50 and 100% UV treated domestic sewage, and quantified effects on growth and development, hepatic energy reserves, and enzymatic pathways associated with detoxification and oxidative stress. Growth and development were accelerated and relative liver size was increased in exposed animals. The exposure resulted in an apparently hormetic increase in hepatic triglycerides and dose-dependent reduction in glycogen stores, as well as increased lipase and NADPH activity, indicating a general disruption to energy metabolism and/or mobilization. Contrary to expectations based on published studies with fish, we found no evidence of lipid peroxidation or induction of the detoxification enzyme Superoxide Dismutase (SOD), however, this may reflect the use of UV treatment as opposed to chlorination for disinfection. Chemical analysis and risk-based prioritization consistently identified fluoxetine, triclosan and diazinon as high-risk contaminants in the wastewater, with nonylphenol and mestranol flagged as risks during one early collection

  6. Stability, transdermal penetration, and cutaneous effects of ascorbic acid and its derivatives.

    Science.gov (United States)

    Stamford, Nicholas P J

    2012-12-01

    Topically applied antioxidants exert their benefits by offering protection from damaging free radicals and over-the-counter cosmeceuticals incorporating antioxidants are among the most popular anti-aging products available. One potent antioxidant of particular note, vitamin C, has been extensively utilized because it possesses a variety of other cutaneous benefits including photoprotection from UV A & B, neocollagenesis, inhibition of melanogenesis and improvement of a variety of inflammatory skin disorders. However, the instability of this water-soluble vitamin, together with difficulties associated with its topical delivery, has presented issues for the formulation chemist. This article reviews the scientific data and clinical studies that underpin the stability, percutaneous absorption, and cutaneous effects of vitamin C together with its commonly utilized, commercially available derivatives.

  7. Quercus suber cork extract displays a tensor and smoothing effect on human skin: an in vivo study.

    Science.gov (United States)

    Coquet, C; Bauza, E; Oberto, G; Berghi, A; Farnet, A M; Ferré, E; Peyronel, D; Dal Farra, C; Domloge, N

    2005-01-01

    Recently, it has become indispensable for anti-aging active ingredients to provide a visible and immediate smoothing antiwrinkle effect. In Quercus suber, suberin is the most important structural component of cork cell walls. Studies have shown that suberin is made up mostly of hydroxycarboxylic acids and that it is endowed with many special mechanical and chemical properties that evoke a possible smoothing effect on the surface of the skin. Therefore, we were interested in investigating the effect of this cork extract on the skin's surface in a double-blind clinical study. The study was conducted in 15 healthy volunteers, aged 22 to 52 years. The volunteers applied a gel formula with 3% of cork extract, or placebo gel, on each forearm. Skin surface roughness was evaluated visually by pictures and by silicone replicas 1 and 2 h after application, followed by statistical analysis using the matched-pairs McNemar statistical test. McNemar analysis of the pictures revealed that application of cork extract on the skin resulted in a highly significant reduction of roughness 1 h after application. This effect was observed in 73.3% of volunteers. Two hours after cork extract application, a highly significant improvement of skin roughness was found in 78.6% of volunteers. Moreover, silicone replica treatment confirmed significant improvement in average of roughness at 2 h. These results demonstrate that cork extract provides a remarkable and highly significant tensor and smoothing effect on the skin, which could be of great use in anti-aging skin care products.

  8. Biological stress response terminology: Integrating the concepts of adaptive response and preconditioning stress within a hormetic dose-response framework.

    Science.gov (United States)

    Calabrese, Edward J; Bachmann, Kenneth A; Bailer, A John; Bolger, P Michael; Borak, Jonathan; Cai, Lu; Cedergreen, Nina; Cherian, M George; Chiueh, Chuang C; Clarkson, Thomas W; Cook, Ralph R; Diamond, David M; Doolittle, David J; Dorato, Michael A; Duke, Stephen O; Feinendegen, Ludwig; Gardner, Donald E; Hart, Ronald W; Hastings, Kenneth L; Hayes, A Wallace; Hoffmann, George R; Ives, John A; Jaworowski, Zbigniew; Johnson, Thomas E; Jonas, Wayne B; Kaminski, Norbert E; Keller, John G; Klaunig, James E; Knudsen, Thomas B; Kozumbo, Walter J; Lettieri, Teresa; Liu, Shu-Zheng; Maisseu, Andre; Maynard, Kenneth I; Masoro, Edward J; McClellan, Roger O; Mehendale, Harihara M; Mothersill, Carmel; Newlin, David B; Nigg, Herbert N; Oehme, Frederick W; Phalen, Robert F; Philbert, Martin A; Rattan, Suresh I S; Riviere, Jim E; Rodricks, Joseph; Sapolsky, Robert M; Scott, Bobby R; Seymour, Colin; Sinclair, David A; Smith-Sonneborn, Joan; Snow, Elizabeth T; Spear, Linda; Stevenson, Donald E; Thomas, Yolene; Tubiana, Maurice; Williams, Gary M; Mattson, Mark P

    2007-07-01

    Many biological subdisciplines that regularly assess dose-response relationships have identified an evolutionarily conserved process in which a low dose of a stressful stimulus activates an adaptive response that increases the resistance of the cell or organism to a moderate to severe level of stress. Due to a lack of frequent interaction among scientists in these many areas, there has emerged a broad range of terms that describe such dose-response relationships. This situation has become problematic because the different terms describe a family of similar biological responses (e.g., adaptive response, preconditioning, hormesis), adversely affecting interdisciplinary communication, and possibly even obscuring generalizable features and central biological concepts. With support from scientists in a broad range of disciplines, this article offers a set of recommendations we believe can achieve greater conceptual harmony in dose-response terminology, as well as better understanding and communication across the broad spectrum of biological disciplines.

  9. Is the integration of hormesis and essentiality into ecotoxicology now opening Pandora's Box?

    Science.gov (United States)

    Kefford, Ben J; Zalizniak, Liliana; Warne, Michael St J; Nugegoda, Dayanthi

    2008-02-01

    Hormesis and essentiality are likely real and common effects at the level of the individual. However, the widespread incorporation of stimulatory effects into applications of ecotoxicology requires the acceptance of assumptions, value judgements and possibly lowering of water/sediment quality standards. There is also currently little data appropriate for considering hormetic effects in the ecotoxicological context. Except perhaps in the case of fitting concentration-response curves, it is not clear that incorporation of hormetic and essentiality type responses into ecotoxicology is necessary. Furthermore, its incorporation presents considerable intellectual and practical changes for ecotoxicology and could have unanticipated consequences.

  10. Effects of Sipgeondaebo-tang Pharmacopuncture Extracts on the Collagenase Activity and Procollagen Synthesis in HS68 Human Fibroblasts and Tyrosinase Activity Original Articles

    Directory of Open Access Journals (Sweden)

    Lee Sena

    2011-03-01

    Full Text Available Objectives: This study was designed to investigate the collagen metabolism and tyrosinase activity of Sipgeondaebo-tang Pharmacopuncture extracts (SP. Methods: The effect of SP on type I procollagen production and collagenase activity in human normal fibroblasts HS68 after UVB (312 nm irradiation was measured by ELISA method. The tyrosinase activity after treatment of SP was measured as well. Results: Type I procollagen production was recovered by SP in UVB damaged HS68 cells. The increased collagenase activity after UVB damage was significantly recovered by SP. The tyrosinase activity was significantly reduced as well. However, the L-DOPA oxidation was not changed. Conclusion: SP showed the anti-wrinkle effects and whitening effects in vitro. These results suggest that SP may be a potential pharmacopuncture as an anti-aging pharmacopuncture treatments.

  11. Advances in Resveratrol on anti-aging%白藜芦醇抗衰老研究现状

    Institute of Scientific and Technical Information of China (English)

    张效莉; 吴景东

    2010-01-01

    白藜芦醇是一种植物抗毒素,主要来源于虎杖、葡萄及花生等植物中,具有抗癌、抗菌、抗氧化等重要的生物功效,尤其在抗衰老方面作用显著,研究进展迅速,开发前景广阔.

  12. The unexpected outcomes of anti-aging, rejuvenation, and life extension studies: an origin of modern therapies.

    Science.gov (United States)

    Stambler, Ilia

    2014-06-01

    The search for life-extending interventions has been often perceived as a purely academic pursuit, or as an unorthodox medical enterprise, with little or no practical outcome. Yet, in fact, these studies, explicitly aiming to prolong human life, often constituted a formidable, though hardly ever acknowledged, motivation for biomedical research and discovery. At least several modern biomedical fields have originated directly from rejuvenation and life extension research: (1) Hormone replacement therapy was born in Charles-Edouard Brown-Séquard's rejuvenation experiments with animal gland extracts (1889). (2) Probiotic diets originated in Elie Metchnikoff's conception of radically prolonged "orthobiosis" (c. 1900). (3) The development of clinical endocrinology owed much to Eugen Steinach's "endocrine rejuvenation" operations (c. 1910s-1920s). (4) Tissue transplantations in humans (allografts and xenografts) were first widely performed in Serge Voronoff's "rejuvenation by grafting" experiments (c. 1910s-1920s). (5) Tissue engineering was pioneered during Alexis Carrel's work on cell and tissue immortalization (c. 1900-1920). (6) Cell therapy (and particularly human embryonic cell therapy) was first widely conducted by Paul Niehans for the purposes of rejuvenation as early as the 1930s. Thus, the pursuit of life extension and rejuvenation has constituted an inseparable and crucial element in the history of biomedicine. Notably, the common principle of these studies was the proactive maintenance of stable, long-term homeostasis of the entire organism.

  13. Sensitivity of Saccharomyces cerevisiae defective in TOR signaling pathway to carbonyl/oxidative stress

    Directory of Open Access Journals (Sweden)

    Valishkevych B. V.

    2014-09-01

    Full Text Available Aim. To investigate the influence of carbonyl/oxidative stress induced by glyoxal, methylglyoxal and hydrogen peroxide on the survival of Saccharomyces cerevisiae, defective for different parts of TOR- signaling pathway, grown on glucose or fructose. Methods. The assessment of number of colony-forming units to determine the yeast reproductive ability. Results. It was shown that at certain concentrations the mentioned above toxicants caused an increase in yeast survival, indicating the hormetic effect. Conclusions. The TOR signaling pathway is involved in the hormetic effect, but it is specific for each strain and depends on the type of carbohydrate in the incubation medium.

  14. Research Progress on Pharmacological Effects of Lycopene%番茄红素药理作用的近五年研究进展

    Institute of Scientific and Technical Information of China (English)

    包华音

    2014-01-01

    番茄红素是一种天然类胡萝卜素,具有抗氧化、延缓衰老、抑制肿瘤、降低血脂、提高人体免疫力、预防心血管疾病、延缓骨质疏松等多种功效。本文综述了近五年番茄红素药理作用的研究进展,为新型药物的开发和应用提供依据。%Lycopene is a kind of natural carotenoids and has effects of anti-oxidation, anti-aging, anti-tumor, preventing hypertension, etc. This paper reviewed the research progress of pharmacological effects of lycopene in recent years, in order to promote the development of new drugs, and to provide reference for further clinical application of lycopene.

  15. Research Progress of Cordyceps militaris' Pharmacological Effect in the Last Five Years%近五年北虫草药理作用研究进展

    Institute of Scientific and Technical Information of China (English)

    金田; 石俊英

    2011-01-01

    Cordyceps militaris is one of common Cordyceps sinensis (Berk) Sacc in China, which has a higher medicinal value. It has the effects of improving the body immunity, anti-fatigue, anti-aging, anti-tumor, etc. This paper reviewed the new progress of research in terms of the Cordyceps militaris' pharmacological effect in the last five years, in order to provide reference for further research and development.%北虫草是我国药用价值较高的虫草菌之一,具有提高人体免疫力、抗疲劳、抗衰老、抗肿瘤等作用.本文综述了近5年北虫草药理作用研究的新进展,为其进一步研究开发提供参考.

  16. Effects of radionuclide contamination on leaf litter decomposition in the Chernobyl exclusion zone

    Energy Technology Data Exchange (ETDEWEB)

    Bonzom, Jean-Marc, E-mail: jean-marc.bonzom@irsn.fr [Institut de Radioprotection et de Sûreté Nucléaire (IRSN), PRP-ENV/SERIS, Cadarache, Bât. 183, BP 3, 13115 St Paul-lez-Durance (France); Hättenschwiler, Stephan [Centre d' Ecologie Fonctionnelle et Evolutive (CEFE UMR 5175, CNRS–Université de Montpellier–Université Paul-Valéry Montpellier–EPHE), 1919 Route de Mende, F-34293 Montpellier (France); Lecomte-Pradines, Catherine [Institut de Radioprotection et de Sûreté Nucléaire (IRSN), PRP-ENV/SERIS, Cadarache, Bât. 183, BP 3, 13115 St Paul-lez-Durance (France); Chauvet, Eric [EcoLab, Université de Toulouse, CNRS, UPS, INPT, 118 Route de Narbonne, 31062 Toulouse cedex (France); Gaschak, Sergey [Chernobyl Center for Nuclear Safety, Radioactive Waste and Radioecology, International Radioecology Laboratory, 07100 Slavutych (Ukraine); Beaugelin-Seiller, Karine; Della-Vedova, Claire; Dubourg, Nicolas [Institut de Radioprotection et de Sûreté Nucléaire (IRSN), PRP-ENV/SERIS, Cadarache, Bât. 183, BP 3, 13115 St Paul-lez-Durance (France); Maksimenko, Andrey [Chernobyl Center for Nuclear Safety, Radioactive Waste and Radioecology, International Radioecology Laboratory, 07100 Slavutych (Ukraine); and others

    2016-08-15

    The effects of radioactive contamination on ecosystem processes such as litter decomposition remain largely unknown. Because radionuclides accumulated in soil and plant biomass can be harmful for organisms, the functioning of ecosystems may be altered by radioactive contamination. Here, we tested the hypothesis that decomposition is impaired by increasing levels of radioactivity in the environment by exposing uncontaminated leaf litter from silver birch and black alder at (i) eleven distant forest sites differing in ambient radiation levels (0.22–15 μGy h{sup −1}) and (ii) along a short distance gradient of radioactive contamination (1.2–29 μGy h{sup −1}) within a single forest in the Chernobyl exclusion zone. In addition to measuring ambient external dose rates, we estimated the average total dose rates (ATDRs) absorbed by decomposers for an accurate estimate of dose-induced ecological consequences of radioactive pollution. Taking into account potential confounding factors (soil pH, moisture, texture, and organic carbon content), the results from the eleven distant forest sites, and from the single forest, showed increased litter mass loss with increasing ATDRs from 0.3 to 150 μGy h{sup −1}. This unexpected result may be due to (i) overcompensation of decomposer organisms exposed to radionuclides leading to a higher decomposer abundance (hormetic effect), and/or (ii) from preferred feeding by decomposers on the uncontaminated leaf litter used for our experiment compared to locally produced, contaminated leaf litter. Our data indicate that radio-contamination of forest ecosystems over more than two decades does not necessarily have detrimental effects on organic matter decay. However, further studies are needed to unravel the underlying mechanisms of the results reported here, in order to draw firmer conclusions on how radio-contamination affects decomposition and associated ecosystem processes. - Highlights: • The effects of radioactivity on

  17. Effect

    OpenAIRE

    M.F. Sabry; M.R. Hamed; El Sayed, M.E.

    2014-01-01

    Stress alters psychological diseases such as anxiety and depression. Protein malnutrition (PM) contributes to psychological disorders. The present study aimed to investigate the effect of biphenyl dimethyl dicarboxylate (DDB) on anxiety of psychologically stressed protein malnourished mice as compared to its effect in normally-fed mice. Fluoxetine (FLX) was used as reference standard. Animals were randomly divided into two major groups, normally-fed group provided with 20% casein diet and a p...

  18. Effect

    OpenAIRE

    Amin Abdou Seleem; Fakhr El-Din M. Lashein

    2016-01-01

    Verapamil is a calcium channel blocker that belongs to the phenylalkylamine group. It has been clinically used for various diseases such as combating hypertension, ischemic heart diseases, supraventricular antiarrhythmic and tycolysis. The study was conducted to investigate the effect of verapamil on selected pro- and apoptotic factors during prenatal retinal differentiation of mice at E14 and E17 of gestation. The pregnant females were classified into two groups, the first is the control and...

  19. 黑蒜的营养价值及保健作用的研究进展%Research progress in the nutritional value and health effects of black garlic

    Institute of Scientific and Technical Information of China (English)

    雷逢超; 郝果; 朱黎; 杨颖; 张有林

    2012-01-01

    The black garlic nutrition, chemical composition and immunity,anti-cancer effects and regulation of blood sugar The mainstream of black garlic as a health food an active role future research directions. its antioxidant, bactericidal, anti-aging, enhancing and other biological functions were summarized. in the human body was introduced,and proposed%本文总结了黑蒜的营养价值、化学成分以及其抗氧化作用、杀菌、抗衰老、增强免疫力、抗癌作用和调节血糖等生物学功能,介绍了黑蒜作为健康食品对人机体的积极作用,提出今后的研究方向。

  20. Selective toxin effects on faster and slower growing individuals in the formation of hormesis at the population level - A case study with Lactuca sativa and PCIB.

    Science.gov (United States)

    Belz, Regina G; Sinkkonen, Aki

    2016-10-01

    Natural plant populations have large phenotypic plasticity that enhances acclimation to local stress factors such as toxin exposures. While consequences of high toxin exposures are well addressed, effects of low-dose toxin exposures on plant populations are seldom investigated. In particular, the importance of 'selective low-dose toxicity' and hormesis, i.e. stimulatory effects, has not been studied simultaneously. Since selective toxicity can change the size distribution of populations, we assumed that hormesis alters the size distribution at the population level, and investigated whether and how these two low-dose phenomena coexist. The study was conducted with Lactuca sativa L. exposed to the auxin-inhibitor 2-(p-chlorophenoxy)-2-methylpropionic acid (PCIB) in vitro. In two separate experiments, L. sativa was exposed to 12 PCIB doses in 24 replicates (50 plants/replicate). Shoot/root growth responses at the population level were compared to the fast-growing (≥90% percentile) and the slow-growing subpopulations (≤10% percentile) by Mann-Whitney U testing and dose-response modelling. In the formation of pronounced PCIB hormesis at the population level, low-dose effects proved selective, but widely stimulatory which seems to counteract low-dose selective toxicity. The selectivity of hormesis was dose- and growth rate-dependent. Stimulation occurred at lower concentrations and stimulation percentage was higher among slow-growing individuals, but partly or entirely masked at the population level by moderate or negligible stimulation among the faster growing individuals. We conclude that the hormetic effect up to the maximum stimulation may be primarily facilitated by an increase in size of the most slow-growing individuals, while thereafter it seems that mainly the fast-growing individuals contributed to the observed hormesis at the population level. As size distribution within a population is related to survival, our study hints that selective effects on slow

  1. Botanical characteristics, pharmacological effects and medicinal compo-nents of Korean Panax ginseng C A Meyer

    Institute of Scientific and Technical Information of China (English)

    Kwang-tae CHOI

    2008-01-01

    Korean Panax ginseng C A Meyer is mainly used to maintain the homeostasis of the body, and the pharmacological efficacy of Korean ginseng identified by modern science includes improved brain function, pain-relieving effects, pre-ventive effects against tumors as well as anti-tumor activity, enhanced immune system function, anti-diabetic effects, enhanced liver function, adjusted blood pressure, anti-fatigue and anti-stress effects, improved climacteric disorder and sexual functions, as well as anti-oxidative and anti-aging effects. Further clini-cal studies of these pharmacological efficacies will continue to be carried out. Korean ginseng is found to have such main properties as ginsenoside, ployacety-lene, acid polysaccharide, anti-oxidative aromatic compound, and insulin-like acid peptides. The number of ginsenoside types contained in Korean ginseng (38 ginsenosides) is substantially more than that of ginsenoside types contained in American ginseng (19 ginsenosides). Furthermore, Korean ginseng has been identified to contain more main non-saponin compounds, phenol compounds, acid polysaccharides and polyethylene compounds than American ginseng and Sanchi ginseng.

  2. Preconditioning is hormesis part I: Documentation, dose-response features and mechanistic foundations.

    Science.gov (United States)

    Calabrese, Edward J

    2016-08-01

    This article provides the first extensive documentation of the dose response features of pre- and postconditioning. Pre- and postconditioning studies with rigorous study designs, using multiple doses/concentrations along with refined dose/concentration spacing strategies, often display hormetic dose/concentration response relationships with considerable generality across biological model, inducing (i.e., conditioning) agent, challenging dose treatment, endpoint, and mechanism. Pre- and postconditioning hormesis dose/concentration-response relationships are reported for 154 diverse conditioning agents, affecting more than 550 dose/concentration responses, across a broad range of biological models and endpoints. The quantitative features of the pre- and postconditioning-induced protective responses are modest, typically being 30-60% greater than control values at maximum, findings that are consistent with a large body (>10,000) of hormetic dose/concentration responses not related to pre- and postconditioning. Regardless of the biological model, inducing agent, endpoint or mechanism, the quantitative features of hormetic dose/concentration responses are similar, suggesting that the magnitude of response is a measure of biological plasticity. This paper also provides the first documentation that hormetic effects account for preconditioning induced early (1-3h) and delayed (12-72h) windows of protection. These findings indicate that pre- and postconditioning are specific types of hormesis.

  3. Inhibitory effect of polysaccharide peptide (PSP) against Tobacco mosaic virus (TMV).

    Science.gov (United States)

    Zhao, Lei; Hao, Xingan; Wu, Yunfeng

    2015-04-01

    Polysaccharides are essential macromolecules present in all living organisms, and have many kinds of biological activities, such as anti-oxidation, hypoglycemic, enhancing immunity, anti-aging, anti-rheumatism, anti-cancer and so on. In this study, the antiviral activity of polysaccharide peptide (PSP) was tested, compared with Ningnanmycin, and firstly found it has a stronger antiviral activity by using half-leaf method and leaf disk method. Subsequently, the mechanism of antiviral activity of PSP was preliminarily studied. As a result, its antiviral effect was better than the commercial agent Ningnanmycin, despite of protective effect, curative effect and inactivation effect. On the other hand, PSP as a commercial anti-cancer drug could easily and rapidly get in large quantities by liquid fermentation, which makes the industrialized production feasible. Also PSP is less toxic, easily biodegradable and ecofriendly. All the results are suggesting that PSP has potential as a pesticide to be used for the control of plant virus in the future.

  4. The Biological Effects of Quadripolar Radiofrequency Sequential Application: A Human Experimental Study

    Science.gov (United States)

    Cornaglia, Antonia Icaro; Faga, Angela; Scevola, Silvia

    2014-01-01

    Abstract Objective: An experimental study was conducted to assess the effectiveness and safety of an innovative quadripolar variable electrode configuration radiofrequency device with objective measurements in an ex vivo and in vivo human experimental model. Background data: Nonablative radiofrequency applications are well-established anti-ageing procedures for cosmetic skin tightening. Methods: The study was performed in two steps: ex vivo and in vivo assessments. In the ex vivo assessments the radiofrequency applications were performed on human full-thickness skin and subcutaneous tissue specimens harvested during surgery for body contouring. In the in vivo assessments the applications were performed on two volunteer patients scheduled for body contouring surgery at the end of the study. The assessment methods were: clinical examination and medical photography, temperature measurement with thermal imaging scan, and light microscopy histological examination. Results: The ex vivo assessments allowed for identification of the effective safety range for human application. The in vivo assessments allowed for demonstration of the biological effects of sequential radiofrequency applications. After a course of radiofrequency applications, the collagen fibers underwent an immediate heat-induced rearrangement and were partially denaturated and progressively metabolized by the macrophages. An overall thickening and spatial rearrangement was appreciated both in the collagen and elastic fibers, the latter displaying a juvenile reticular pattern. A late onset in the macrophage activation after sequential radiofrequency applications was appreciated. Conclusions: Our data confirm the effectiveness of sequential radiofrequency applications in obtaining attenuation of the skin wrinkles by an overall skin tightening. PMID:25244081

  5. Neuroprotective effects of ginsenoside Rb1 on hippocampal neuronal injury and neurite outgrowth

    Institute of Scientific and Technical Information of China (English)

    Juan Liu; Jing He; Liang Huang; Ling Dou; Shuang Wu; Qionglan Yuan

    2014-01-01

    Ginsenoside Rb1 has been reported to exert anti-aging and anti-neurodegenerative effects. In the present study, we investigate whether ginsenoside Rb1 is involved in neurite outgrowth and neuroprotection against damage induced by amyloid beta (25-35) in cultured hippocampal neu-rons, and explore the underlying mechanisms. Ginsenoside Rb1 significantly increased neurite outgrowth in hippocampal neurons, and increased the expression of phosphorylated-Akt and phosphorylated extracellular signal-regulated kinase 1/2. These effects were abrogated by API-2 and PD98059, inhibitors of the signaling proteins Akt and MEK. Additionally, cultured hippo-campal neurons were exposed to amyloid beta (25-35) for 30 minutes; ginsenoside Rb1 prevented apoptosis induced by amyloid beta (25-35), and this effect was blocked by API-2 and PD98059. Furthermore, ginsenoside Rb1 significantly reversed the reduction in phosphorylated-Akt and phosphorylated extracellular signal-regulated kinase 1/2 levels induced by amyloid beta (25-35), and API-2 neutralized the effect of ginsenoside Rb1. The present results indicate that ginsenoside Rb1 enhances neurite outgrowth and protects against neurotoxicity induced by amyloid beta (25-35) via a mechanism involving Akt and extracellular signal-regulated kinase 1/2 signaling.

  6. Antioxidant Activity, Antitumor Effect, and Antiaging Property of Proanthocyanidins Extracted from Kunlun Chrysanthemum Flowers

    Directory of Open Access Journals (Sweden)

    Siqun Jing

    2015-01-01

    Full Text Available The objective of the present study was to evaluate the antioxidant activity, antitumor effect, and antiaging property of proanthocyanidins from Kunlun Chrysanthemum flowers (PKCF grown in Xinjiang. In vitro antioxidant experiments results showed that the total antioxidant activity and the scavenging capacity of hydroxyl radicals (•OH and 1,1-diphenyl-2-picrylhydrazyl (DPPH• radicals increased in a concentration-dependent manner and were stronger than those of vitamin C. To investigate the antioxidant activity of PKCF in vivo, we used serum, liver, and kidney from mouse for the measurement of superoxide dismutase (SOD, malondialdehyde (MDA, and total antioxidant capacity (T-AOC. Results indicated that PKCF had antioxidative effect in vivo which significantly improved the activity of SOD and T-AOC and decreased MDA content. To investigate the antitumor activity of PKCF, we used H22 cells, HeLa cells, and Eca-109 cells with Vero cells as control. Inhibition ratio and IC50 values were measured by 3-(4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide (MTT assay; PKCF showed great inhibitory activity on H22 cells and HeLa cells. We also used fruit flies as a model for analyzing the anti-aging property of PKCF. Results showed that PKCF has antiaging effect on Drosophila. Results of the present study demonstrated that PKCF could be a promising agent that may find applications in health care, medicine, and cosmetics.

  7. Parabens inhibit human skin estrogen sulfotransferase activity: possible link to paraben estrogenic effects.

    Science.gov (United States)

    Prusakiewicz, Jeffery J; Harville, Heather M; Zhang, Yanhua; Ackermann, Chrisita; Voorman, Richard L

    2007-04-11

    Parabens (p-hydroxybenzoate esters) are a group of widely used preservatives in topically applied cosmetic and pharmaceutical products. Parabens display weak associations with the estrogen receptors in vitro or in cell based models, but do exhibit estrogenic effects in animal models. It is our hypothesis that parabens exert their estrogenic effects, in part, by elevating levels of estrogens through inhibition of estrogen sulfotransferases (SULTs) in skin. We report here the results of a structure-activity-relationship of parabens as inhibitors of estrogen sulfation in human skin cytosolic fractions and normal human epidermal keratinocytes. Similar to reports of paraben estrogenicity and estrogen receptor affinity, the potency of SULT inhibition increased as the paraben ester chain length increased. Butylparaben was found to be the most potent of the parabens in skin cytosol, yielding an IC(50) value of 37+/-5 microM. Butylparaben blocked the skin cytosol sulfation of estradiol and estrone, but not the androgen dehydroepiandrosterone. The parabens were also tested as inhibitors of SULT activity in a cellular system, with normal human epidermal keratinocytes. The potency of butylparaben increased three-fold in these cells relative to the IC(50) value from skin cytosol. Overall, these results suggest chronic topical application of parabens may lead to prolonged estrogenic effects in skin as a result of inhibition of estrogen sulfotransferase activity. Accordingly, the skin anti-aging benefits of many topical cosmetics and pharmaceuticals could be derived, in part, from the estrogenicity of parabens.

  8. Memory-enhancing effect of Mori Fructus via induction of nerve growth factor.

    Science.gov (United States)

    Kim, Hyo Geun; Oh, Myung Sook

    2013-07-14

    Fruits rich in phytochemicals have been shown to improve memory by protecting or enhancing neuronal functions mediated by neurotrophic factors, such as nerve growth factor (NGF), in the hippocampus. Mori Fructus (Morus alba L., Moraceae), also called mulberry, is used as a food, dietary supplement and an anti-ageing agent in traditional Oriental medicine. It is also known to contain abundant flavonoid compounds and to exhibit various pharmacological effects. The present study was performed to evaluate the memory-enhancing effect of Mori Fructus extract (ME) in mice, with a focus on NGF regulation. ME (20, 100 and 500 mg/kg per d for 7 d, per os) dose-dependently promoted NGF release in the mouse hippocampus, leading to phosphorylation of extracellular signal-regulated kinases and cyclic AMP response element-binding protein. ME significantly increased pre- and post-synapse formation, acetylcholine synthesisation, neuronal cell differentiation, neurite outgrowth and neuronal cell proliferation in the mouse hippocampus. Furthermore, ME significantly increased latency time in the passive avoidance task (Pimprovements in learning and memory. Taken together, these data suggest that ME exhibits a memory-enhancing effect via up-regulation of NGF.

  9. Effects of Moxa Smoke on Monoamine Neurotransmitters in SAMP8 Mice

    Directory of Open Access Journals (Sweden)

    Huanfang Xu

    2013-01-01

    Full Text Available Objectives. To investigate the anti-aging effects of moxa smoke on SAMP8 mice. Methods. Using 2×3 factorial design, exposure length (15 or 30 minutes daily, and concentration (low, 5–15 mg/m3; middle, 25–35 mg/m3; high, 85–95 mg/m3, 70 SAMP8 mice were randomly assigned, n=10/group, to a model group or one of six moxa smoke groups: L1, L2, M1, M2, H1, or H2. Ten SAMR1 mice were used as normal control. Mice in moxa smoke groups were exposed to moxa smoke at respective concentrations and exposure lengths; the model and normal control mice were not exposed. Cerebral 5-HT, DA, and NE levels were determined using ELISA. Results. Compared to normal control, the model group showed a significant decrease in 5-HT, DA, and NE. Compared to model group, 5-HT and NE were significantly higher in groups L2, M1, and M2 and DA was significantly so in L2 and M1. 5-HT, DA, and NE levels were the highest in group M1 among moxa smoke groups. A marked exposure length × concentration interaction was observed for 5-HT, DA, and NE. Conclusion. Moxa smoke increases monoamine neurotransmitter levels, which varies according to concentration and exposure length. Our finding suggests that the middle concentration of moxa smoke for 15 minutes seems the most beneficial.

  10. Functional evaluation for effective compositions in seed oil of Korean pine

    Institute of Scientific and Technical Information of China (English)

    WANGZhen-yu; CHENXiao-qiang

    2004-01-01

    The effect of seed oil of Korean pine (Pinus koraiensis) on the rats' blood·fat and its anti·ageing function was studied for appraising the efficacy of the seed oil of Korean pine. Sixty experimental rats were randomly divided into 5 groups (half males and half females in each group) as normal control group, high fat diet control group, and three groups (Group 1 Group 2,Group 3) that were fed with feedstuff with the contents of the seed oil of 2.0g/(kg· d-1), 4.0g/(kg· d1) and 8.0g/(kg· d-1), respectively. The indexes such as superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) and anti-oxidation capacity (AOC) were measured by Reagent Kit method. It was found that the seed oil of the Korean pine could reduce the content of triglyceride and improve SOD as well as GSH-PX activity in serum. These indexes of the rats in Group 2 fed with Korean pine seed oil of 4.0 g/(kg·d-1) reached the significant level and those of rats in Group 3 fed with the seed oil of 8.0 g/(kg·d-1) reached the extremely significant level. The results indicated the seed oil of Korean pine had function of regulating the level of blood-fat and anti-ageing.

  11. Life Span and Motility Effects of Ethanolic Extracts from Sophora moorcroftiana Seeds on Caenorhabditis elegans

    Science.gov (United States)

    Li, Xin; Han, Junxian; Zhu, Rongyan; Cui, Rongrong; Ma, Xingming; Dong, Kaizhong

    2016-01-01

    Background: Sophora moorcroftiana is an endemic shrub species with a great value in folk medicine in Tibet, China. In this study, relatively little is known about whether S. moorcroftiana is beneficial in animals' nervous system and life span or not. Materials and Methods: To address this question, under survival normal temperature (25°C), S. moorcroftiana seeds were extracted with 95% ethanol, and Caenorhabditis elegans were exposed to three different extract concentrations (100 mg/L, 200 mg/L, and 400 mg/mL) from S. moorcroftiana seeds. Results: The 95% ethanolic extracts from S. moorcroftiana seeds could increase life span and slow aging-related increase in C. elegans and could not obviously influence the motility of C. elegans. Conclusion: Given these results by our experiment for life span and motility with 95% ethanolic extracts from S. moorcroftiana seeds in C. elegans, the question whether S. moorcroftiana acts as an anti-aging substance in vivo arises. SUMMARY The 95% ethanolic extracts from S. moorcroftiana seeds have no effect on the life span in C. elegans when extract concentrations from S. moorcroftiana seeds <400 mg/LThe 400 mg/L 95% ethanolic extracts from S. moorcroftiana seeds could increase life span in C. elegansThe 95% ethanolic extracts from S. moorcroftiana seeds could not obviously influence the motility in C. elegans. Abbreviation used: S. moorcroftiana: Sophora moorcroftiana; C. elegan: Caenorhabditis elegan; E. coli OP50: Escherichia coli OP50; DMSO: Dimethyl sulfoxide. PMID:27279712

  12. Antiproliferative activity and apoptotic effects of Filipendula ulmaria pollen against C26 mice colon tumour cells

    Directory of Open Access Journals (Sweden)

    Mărgăoan Rodica

    2016-06-01

    Full Text Available Honeybee collected pollen exhibits high nutritional and pharmaceutical benefits for the human diet and medicine. Pollen’s antioxidant, anti-ageing, anti-inflammatory, anti-atherosclerosis, and cardioprotective activity, depending on the floral origin, are well known. Recent studies proposed that pollen may also be an excellent cancer-fighting candidate, as pollen harbours high amounts of phenolic substances. In our study, Filipendula ulmaria pollen (bee collected was methanol-water extracted and used to verify its in vitro pharmacological activities on C26 mice cancer tumour cells. Three different concentrations of the extract were tested in antitumour assays. Monitoring was done after 6, 12, 24, and 48 hours. Promising results were obtained for antiproliferative and apoptotic activity of the pollen extracts, with high efficiency for the highest concentration (1 mg/mL. For both activities, time and concentration-dependent effects were observed. Pollen extracts or bee collected pollen has a high potential as an antitumour agent for use in human medicine, because they are both rich in bioactive compounds.

  13. 防头发老化及损伤的洗护发产品%Hair shampoo and conditioner with anti-aging and damage proof

    Institute of Scientific and Technical Information of China (English)

    程伟; 李恩泽

    2013-01-01

    对洗发水和护发素的市场现状进行了分析.介绍了一些防头发老化的新产品,并针对女性的生理特征来处理头发变稀和脱发现象,从而从根本上解决因年龄而出现的头发问题.头发的修复是头发护理品市场中的一个重要部分.介绍了一些能够帮助修复、恢复和保护头发的新产品.

  14. 热量限制与白藜芦醇抗衰老的研究进展%Recent Anti-aging Studies on Caloric Restriction and Resveratrol

    Institute of Scientific and Technical Information of China (English)

    郭燕世

    2011-01-01

    Seventy-five years after its initial report, lifespan prolongation by caloric restriction (CR) has been confirmed in many animal species. Gathered evidences suggest that the histone deacetylase Sir2/ SIRT1 is a key mediator. Moreover, resveratrol (RSV) , a plant derived polyphenol, was shown to increase SIRT1 catalytic activity and mimics many aspects of CR in all eukaryotes tested. Therefore, RSV displays great promise in the prevention of illness and delay the aging process.%限制热量摄取(caloric restriction,CR) 能够延长寿命,这个75 年前的发现己得到广泛的证明.其作用主要是由去乙酰化酶 (Sir2/SIRT1)所介导.近年又发现,植物中的多酚化物--白藜芦醇,是SIRT1 的激活剂,它能够模拟 CR 的抗衰老作用,并能防治多种疾病.因此它有可能成为人类防病和抗衰老的有用工具.

  15. Research on Resveratrol's Mechanism of Immunity in Anti-aging%白藜芦醇抗衰老免疫机制的研究

    Institute of Scientific and Technical Information of China (English)

    姚煜; 田涛; 南克俊

    2006-01-01

    目的:研究白藜芦醇抗衰老的免疫学机制,开发白藜芦醇新的临床用途.方法:建立小鼠衰老模型,然后用白藜芦醇干预,观察其对小鼠衰老现象的影响作用.结果:白藜芦醇干预组小鼠血清超氧化物歧化酶(SOD)含量升高,血清丙二醛(MDA)含量下降;该组胸腺指数升高而脾脏指数无明显改变;同时其血清中CD4+细胞数量不改变,而CD8+细胞数量升高,CD8+细胞与CD4+细胞比值降低;还能使血清中IL-6、IL-8的含量降低.结论:白藜芦醇具有一定的抗衰老作用.

  16. The role of COX-2 and Nrf2/ARE in anti-inflammation and antioxidative stress: Aging and anti-aging.

    Science.gov (United States)

    Luo, Cheng; Urgard, Egon; Vooder, Tõnu; Metspalu, Andres

    2011-08-01

    Oxidative stress and inflammation are constant features of many chronic diseases and complications, and have been linked to carcinogenesis. Cyclooxygenase 2 (COX-2), a rate-limiting enzyme for the synthesis of prostaglandins, plays important roles in physiology and pathology, but has been a source of controversy within the scientific and clinical community. However, recent work has shown that nuclear factor erythroid-2-related factor-2 (Nrf2) confers protection against oxidative stress. Furthermore, COX-2-dependent electrophile oxo-derivative (EFOX) molecules have been shown to act as anti-inflammatory mediators via activation of the Nrf2-dependent antioxidant response element (ARE). These studies have provided more insight into COX-2-mediated events. The function of all tissues, especially epithelial and endothelial tissues, declines with age, leading to the production of reactive oxygen species (ROS). COX-2 expression increases with aging in most tissues, due in part to ROS, chemical reactions, physical shearing, and dietary molecules. Here we discuss new findings related to COX-2 inflammatory and anti-inflammatory responses. Taken together, we hypothesize that COX-2 levels increase during the aging process because increasing levels of ROSs necessitate the involvement of COX-2-dependent EFOXs for anti-inflammation and Nrf2/ARE signaling for antioxidation. We also propose that COX-2 may act as an intrinsic biological aging clock due to its role in balancing inflammatory and anti-inflammatory responses.

  17. Skin anti-aging benefits of brown seaweed extract%褐藻萃取物的皮肤抗衰老效果

    Institute of Scientific and Technical Information of China (English)

    刘晓英

    2012-01-01

    皮肤老化会引起皮肤的基因表达水平的改变,从而影响皮肤超微细结构的完整性,引起皮肤干燥、松弛以及出现皱纹等现象。褐藻萃取物具有恢复皮肤成纤维细胞中特殊基因表达值的作用,进而提高皮肤的屏障机能以及皮肤的水合作用,增进皮肤的弹性,减少皱纹的产生。%Aging triggers the modification of gcne expression levels, negativcly affecting skin's ultra structural integrit) and resulting in dr3T, saggy skin and the appearance of wrinkles. However, an extract of brovcn seaweed saccharine longicruris has been found to restore the expression levels of specific genes in senescent fibroblasts, in turn imprfwing barrier function and hydration, increasing elasticity and reducing wrinkles, which are described here.

  18. Preparation of transparent fluorocarbon/TiO2-SiO2 composite coating with improved self-cleaning performance and anti-aging property

    Science.gov (United States)

    Zhou, Jianping; Tan, Zhongyuan; Liu, Zhilei; Jing, Mengmeng; Liu, Wenjie; Fu, Wanli

    2017-02-01

    This work reports a facile method to fabricate transparent self-cleaning fluorocarbon coatings filled by semicrystalline colloidal particles of TiO2-SiO2 composite oxide presenting a particle size ranging from 6 to 10 nm. Anatase-TiO2 crystallites were successfully obtained after microwave heating treatment of the TiO2-SiO2 colloidal particles as confirmed by XRD, TEM and FTIR measurements. The fluorocarbon/TiO2-SiO2 composite coatings exhibited a superior hydrophilicity and an improved photocatalytic activity in contrast to the TiO2-filled coatings. In particular, a water contact angle (WCA) value of 4.5° and a decolorization ratio relative to methyl orange as high as 96.0% were achieved for the composite coatings containing 1.5 wt% of TiO2-SiO2 colloidal particles. The results of the anti-soiling experiments indicated that the fluorocarbon/TiO2-SiO2 composite coatings exhibited a prominent self-cleaning performance, while the accelerated aging experiments revealed that the fluorocarbon/TiO2-SiO2 composite coatings were highly stable toward UV irradiation when compared to the TiO2-filled fluorocarbon coatings. These findings indicated that the fluorocarbon/TiO2-SiO2 composite coatings could be a very attractive solution for many practical areas, especially for outdoor applications.

  19. Mutation Breeding of Anti-aging Beer Yeast Strains%抗老化啤酒酵母菌株的诱变选育

    Institute of Scientific and Technical Information of China (English)

    赵换英; 陈叶福; 王晓琼; 秦伟军; 郭学武; 肖冬光

    2009-01-01

    谷胱甘肽(GSH)在啤酒中具有抗老化作用.选育高产谷胱甘肤的啤酒酵母菌株有助于改善啤酒抗老化性能.以啤酒酵母S5为出发菌株,经紫外诱变和硫酸二乙酯(DES)诱变后,获得突变株SC67,其GSH胞内生成量为15.238 mg/L,胞内含量为11.292 mg/g,较S5分别提高91.4%和57.8%.SC67发酵所得啤酒中胞外谷胱甘肽含量(6.43 mg/L)较S5(1.35 mg/L)显著提高,啤酒抗氧化性能得到明显改善,DPPH自由基清除率较S5提高13.2%;TBA值下降7.1%;RSV值提高49.2%.

  20. A novel flexible clinical multiphoton tomograph for early melanoma detection, skin analysis, testing of anti-age products, and in situ nanoparticle tracking

    Science.gov (United States)

    Weinigel, Martin; Breunig, Hans Georg; Gregory, Axel; Fischer, Peter; Kellner-Höfer, Marcel; Bückle, Rainer; König, Karsten

    2010-02-01

    High-resolution 3D microscopy based on multiphoton induced autofluorescence and second harmonic generation have been introduced in 1990. 13 years later, CE-marked clinical multiphoton systems for 3D imaging of human skin with subcellular resolution have first been launched by JenLab company with the tomography DermaInspect®. This year, the second generation of clinical multiphoton tomographs was introduced. The novel multiphoton tomograph MPTflex, equipped with a flexible articulated optical arm, provides an increased flexibility and accessibility especially for clinical and cosmetical examinations. Improved image quality and signal to noise ratio (SNR) are achieved by a very short source-drain spacing, by larger active areas of the detectors and by single photon counting (SPC) technology. Shorter image acquisition time due to improved image quality reduces artifacts and simplifies the operation of the system. The compact folded optical design and the light-weight structure of the optical head eases the handling. Dual channel detectors enable to distinguish between intratissue elastic fibers and collagenous structures simultaneously. Through the use of piezo-driven optics a stack of optical cross-sections (optical sectioning) can be acquired and 3D imaging can be performed. The multiphoton excitation of biomolecules like NAD(P)H, flavins, porphyrins, elastin, and melanin is done by picojoule femtosecond laser pulses from an tunable turn-key femtosescond near infrared laser system. The ability for rapid high-quality image acquisition, the user-friendly operation of the system and the compact and flexible design qualifies this system to be used for melanoma detection, diagnostics of dermatological disorders, cosmetic research and skin aging measurements as well as in situ drug monitoring and animal research.

  1. 室外涂饰木材的抗老化性能%Research on Anti-aging Ability of Exterior Coated Wood

    Institute of Scientific and Technical Information of China (English)

    陆步云; MERLIN; A.; PERRE; P.; CHRUSCIEL; L.; 周定国

    2007-01-01

    通过测量气体渗透值研究室外涂饰木材在老化过程中的性能表现.结果表明:涂层渗透性与涂料的抗老化能力无直接关系;木材涂饰以后气体渗透值比未涂饰时均有所下降,在老化过程中涂饰木材的气体渗透值随着老化时间的增加而增加.木材径切面上的漆膜比弦切面上的抗老化,径切面上的渗透变化率kt和老化时间的关系为:渗透变化率在老化初期增长趋势很快,随着老化时间的增加逐渐变缓,到老化后期甚至出现渗透变化率下降的趋势.

  2. The Protecting Effect of Deoxyschisandrin and Schisandrin B on HaCaT Cells against UVB-Induced Damage.

    Directory of Open Access Journals (Sweden)

    Wei Hou

    Full Text Available Schisandra chinensis is a traditional Chinese medicine that has multiple biological activities, including antioxidant, anticancer, tonic, and anti-aging effects. Deoxyschisandrin (SA and schisandrin B (SB, the two major lignans isolated from S. chinensis, exert high antioxidant activities in vitro and in vivo by scavenging free radicals, such as reactive oxygen species (ROS. Ultraviolet B-ray (UVB radiation induces the production of ROS and DNA damage, which eventually leads to cell death by apoptosis. However, it is unknown whether SA or SB protects cells against UVB-induced cellular DNA damage. Our study showed that both SA and SB effectively protected HaCaT cells from UVB-induced cell death by antagonizing UVB-mediated production of ROS and induction of DNA damage. Our results showed that both SA and SB significantly prevented UVB-induced loss of cell viability using 3-(4, 5-dimethylthiazol-2-yl-2, 5-diphenyltetrazolium bromide (MTT assays. Dichloro-dihydro-fluorescein diacetate (DCFH-DA assays showed that the production of ROS following UVB exposure was inhibited by treatment with SA and SB. Moreover, SA and SB decreased the UVB-induced DNA damage in HaCaT cells by comet assays. In addition, SA and SB also prevented UVB-induced cell apoptosis and the cleavage of caspase-3, caspase-8 and caspase-9. In a word, our results imply that the antioxidants SA and SB could protect cells from UVB-induced cell damage via scavenging ROS.

  3. The Protecting Effect of Deoxyschisandrin and Schisandrin B on HaCaT Cells against UVB-Induced Damage.

    Science.gov (United States)

    Hou, Wei; Gao, Wei; Wang, Datao; Liu, Qingxiu; Zheng, Siwen; Wang, Yingping

    2015-01-01

    Schisandra chinensis is a traditional Chinese medicine that has multiple biological activities, including antioxidant, anticancer, tonic, and anti-aging effects. Deoxyschisandrin (SA) and schisandrin B (SB), the two major lignans isolated from S. chinensis, exert high antioxidant activities in vitro and in vivo by scavenging free radicals, such as reactive oxygen species (ROS). Ultraviolet B-ray (UVB) radiation induces the production of ROS and DNA damage, which eventually leads to cell death by apoptosis. However, it is unknown whether SA or SB protects cells against UVB-induced cellular DNA damage. Our study showed that both SA and SB effectively protected HaCaT cells from UVB-induced cell death by antagonizing UVB-mediated production of ROS and induction of DNA damage. Our results showed that both SA and SB significantly prevented UVB-induced loss of cell viability using 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assays. Dichloro-dihydro-fluorescein diacetate (DCFH-DA) assays showed that the production of ROS following UVB exposure was inhibited by treatment with SA and SB. Moreover, SA and SB decreased the UVB-induced DNA damage in HaCaT cells by comet assays. In addition, SA and SB also prevented UVB-induced cell apoptosis and the cleavage of caspase-3, caspase-8 and caspase-9. In a word, our results imply that the antioxidants SA and SB could protect cells from UVB-induced cell damage via scavenging ROS.

  4. Targeting the age-related occurrence, removal, and accumulation of molecular damage by hormesis.

    Science.gov (United States)

    Rattan, Suresh I S

    2010-06-01

    Strategies for testing and developing effective means of intervention, prevention, and modulation of aging incorporate means to minimize the occurrence and accumulation of molecular damage, to reduce molecular heterogeneity, and to evaluate the relevance of the type and extent of damage with respect to its role in aging and age-related diseases. One such approach is that of mild stress-induced hormesis, which stimulates maintenance and repair systems and strengthens the homeodynamic space of cells and organisms. Hormesis through mild heat shock, natural and synthetic hormetins, and other stressors brings about several antiaging effects in human fibroblasts, keratinocytes, and telomerase-immortalized bone marrow stem cells. Depending on the cell type, these antiaging hormetic effects include extension of replicative life span, enhanced proteasomal activities, increased chaperone levels, and improved wound healing, angiogenesis, and differentiation. The main molecular pathways for achieving such hormetic effects are through targeting the processes for the repair and removal of molecular damage, which can slow aging.

  5. Effect of Additive on Properties of Proso Millet Starch Paste%添加剂对糜子淀粉糊性质的影响

    Institute of Scientific and Technical Information of China (English)

    王颖; 晁桂梅; 杨秋歌; 高金锋; 高小丽; 王鹏科; 屈洋; 冯佰利

    2012-01-01

    Starch were extracted from yumi3 proso millet by alkaline method and study on effects of additive ( sucrose, sodium carbonate, alum ) on freeze - thaw stability, retrogradation and pasting properties of proso millet starch paste. The results showed that adding to sucrose, sodium carbonate and alum, the freez - thaw stability and retrogradation of proso millet starch paste were improved. But alum had significant effect on freez - thaw stability and retrogradation of proso millet starch paste. Compared with control, adding to alum, the freez - thaw stability and ret-rogradation of proso millet starch paste were increased 40. 7% and 78. 8% . Sucrose can improve viscosity but weaken gelation and anti - aging ability of starch paste. Sodium carbonate and alum can improve gelation and anti - aging a-bility of proso millet starch paste. The results provide a theoretical foundation for the proso millet starch paste's properties and it further application in frozen products.%以榆糜3号为材料,采用碱提法制备淀粉,研究了蔗糖、碳酸钠和明矾对糜子淀粉糊冻融稳定性、凝沉性和糊化特性的影响.结果表明,3种添加剂均改善了糜子淀粉糊的冻融稳定性和凝沉性,其中明矾对糜子淀粉糊的冻融稳定性和凝沉性影响极显著,与对照相比分别提高了40.7%和78.8%;蔗糖的存在使糜子淀粉糊的黏度增加,成胶能力和抗老化能力减弱;碳酸钠、明矾的存在均使糜子淀粉糊的成胶能力和抗老化能力增强.

  6. Antioxidant effects of the orientin and vitexin in Trollius chinensis Bunge in D-galactose-aged mice

    Institute of Scientific and Technical Information of China (English)

    Fang An; Guodong Yang; Jiaming Tian; Shuhua Wang

    2012-01-01

    Total flavonoids are the main pharmaceutical components of Trollius chinensis Bunge,and orientin and vitexin are the monomer components of total flavonoids in Trollius chinensis Bunge.In this study,an aged mouse model was established through intraperitoneal injection of D-galactose for 8 weeks,followed by treatment with 40,20,or 10 mg/kg orientin,vitexin,or a positive control (vitamin E)via intragastric administration for an additional 8 weeks.Orientin,vitexin,and vitamin E improved the general medical status of the aging mice and significantly increased their brain weights.They also produced an obvious rise in total antioxidant capacity,superoxide dismutase,catalase,and glutathione peroxidase levels in the serum,and the levels of superoxide dismutase,catalase and glutathione peroxidase,Na+-K+-ATP enzyme,and Ca2+-Mg2+-ATP enzyme in the liver,brain and kidneys.In addition,they significantly reduced malondialdehyde levels in the liver,brain and kidney and lipofuscin levels in the brain.They alsosignificantly improved the neuronal ultrastructure.The 40 mg/kg dose of orientin and vitexin had the same antioxidant capacity as vitamin E.These experimental findings indicate that orientin and vitexin engender anti-aging effects through their antioxidant capacities.

  7. Antioxidant effects of the orientin and vitexin in Trollius chinensis Bunge in D-galactose-aged mice.

    Science.gov (United States)

    An, Fang; Yang, Guodong; Tian, Jiaming; Wang, Shuhua

    2012-11-25

    Total flavonoids are the main pharmaceutical components of Trollius chinensis Bunge, and orientin and vitexin are the monomer components of total flavonoids in Trollius chinensis Bunge. In this study, an aged mouse model was established through intraperitoneal injection of D-galactose for 8 weeks, followed by treatment with 40, 20, or 10 mg/kg orientin, vitexin, or a positive control (vitamin E) via intragastric administration for an additional 8 weeks. Orientin, vitexin, and vitamin E improved the general medical status of the aging mice and significantly increased their brain weights. They also produced an obvious rise in total antioxidant capacity, superoxide dismutase, catalase, and glutathione peroxidase levels in the serum, and the levels of superoxide dismutase, catalase and glutathione peroxidase, Na(+)-K(+)-ATP enzyme, and Ca(2+)-Mg(2+)-ATP enzyme in the liver, brain and kidneys. In addition, they significantly reduced malondialdehyde levels in the liver, brain and kidney and lipofuscin levels in the brain. They also significantly improved the neuronal ultrastructure. The 40 mg/kg dose of orientin and vitexin had the same antioxidant capacity as vitamin E. These experimental findings indicate that orientin and vitexin engender anti-aging effects through their antioxidant capacities.

  8. The Multiple Effect of Estrogen Receptor on Skin Antiaging%雌激素受体在抗皮肤老化过程中的多效性

    Institute of Scientific and Technical Information of China (English)

    朱焱; 宋智琦; 赵春晖; 邹伟

    2012-01-01

    The estrogen plays an important role in skin antiaging. The effects of estrogen are predominantly mediated through the estrogen receptors (ER). The ER isoforms regulate multiple signaling pathways. The distribution of ER in skin cell not only have difference in quantity but in type. The differentation and gene polymorphism have lead to the multiple effect of ER. In this paper, the anti aging ability of ER isoforms which are in different layer of skin will be summarized and discussed.%雌激素在抗皮肤老化过程中起着重要作用,其通过与皮肤上的雌激素受体结合发挥作用.不同亚型的雌激素受体介导不同的信号传导通路.皮肤细胞中雌激素受体的分布不仅存在数量差异更有类别差异,这些差异性和雌激素受体本身的基因多态性决定了其作用的多效性.简要综述了不同亚型雌激素受体在各皮肤解剖层次中抗老化作用的研究进展.

  9. Effects on the Human Body of a Dietary Supplement Containing L-Carnitine and Garcinia cambogia Extract: A Study using Double-blind Tests.

    Science.gov (United States)

    Yonei, Yoshikazu; Takahashi, Yoko; Hibino, Sawako; Watanabe, Miwako; Yoshioka, Toshito

    2008-03-01

    The effect of a dietary supplement with L-carnitine (600 mg/day) and Garcinia cambogia extract (500 mg/day as hydroxycitric acid) as main ingredients was studied in 35 healthy volunteers {48.3 +/- 6.9 years, body mass index (BMI): 26.3 +/- 1.7} in a double-blind test (18 subjects in the Test Group and 17 in the Control Group). The yearly examination includes the standard yearly medical tests done in Japan, tests for assessing hormonal age, and a survey for assessing physical and mental fitness of the subjects, called the Anti-Aging QOL Common Questionnaire (AAQol). Use of this supplement significantly improved the level of lipid peroxides (-12.8%) in the blood as well as physical symptoms such as "tired eyes," "blurry eyes," "muscle pain/stiffness," "early satiety," "epigastralgia," "dizziness," "arthralgia" and "easily breaking into a sweat." The Control Group showed a significantly favorable improvement rate, especially for "dizziness." On the other hand, groups of subjects using the test compounds saw a significant rise in total cholesterol (4.5%), fasting blood sugar (4.1%) and HbA1c (3.4%). Our findings suggest that the consumption of the supplement can reduce the oxidative damage; however, the effect on QOL was equivocal. Garcinia cambogia extract did not show dietary efficacy.

  10. Anti-Skin-Aging Effect of Epigallocatechin Gallate by Regulating Epidermal Growth Factor Receptor Pathway on Aging Mouse Model Induced by D-Galactose.

    Science.gov (United States)

    Chen, Jiming; Li, Yifan; Zhu, Qiangqiang; Li, Tong; Lu, Hao; Wei, Nan; Huang, Yewei; Shi, Ruoyu; Ma, Xiao; Wang, Xuanjun; Sheng, Jun

    2017-03-23

    Epigallocatechin gallate(EGCG) is a monomer separated from tea catechins, as an well-known antioxidant, which helps fight wrinkles and rejuvenate skin cells. In this study, we investigated the anti-aging effect of EGCG, and to clarify underlying mechanism of skin aging in a D-galactose-induced aging mouse model. Forty-five male mice were divided into 5 groups and treated with different dose of EGCG, Vitamin C (VitC) to mice as a positive control. All groups except vehicle were established aging model induced by D-galactose (200mg/kg/day) that was subcutaneously injected to mice for 8 weeks. Two weeks after injection of D-galactose, EGCG and Vit C groups were simultaneously administered once a day by subcutaneously inject after 5hours for injecting D-galactose. The results show that EGCG can be absorbed by the skin. Overall, the conditions of the skin of EGCG-treatment groups were improved, the whole structure of skin were better than control groups, and the levels of oxidative stress and the expression of relate with EGFR proteins were significantly higher than control group after EGCG treatment. All these findings suggest that EGCG can resist skin senility effectively. And the EGFR with relate of downstream proteins are implicated in the skin aging.

  11. Oral supplementation of specific collagen peptides has beneficial effects on human skin physiology: a double-blind, placebo-controlled study.

    Science.gov (United States)

    Proksch, E; Segger, D; Degwert, J; Schunck, M; Zague, V; Oesser, S

    2014-01-01

    Various dietary supplements are claimed to have cutaneous anti-aging properties; however, there are a limited number of research studies supporting these claims. The objective of this research was to study the effectiveness of collagen hydrolysate (CH) composed of specific collagen peptides on skin biophysical parameters related to cutaneous aging. In this double-blind, placebo-controlled trial, 69 women aged 35-55 years were randomized to receive 2.5 g or 5.0 g of CH or placebo once daily for 8 weeks, with 23 subjects being allocated to each treatment group. Skin elasticity, skin moisture, transepidermal water loss and skin roughness were objectively measured before the first oral product application (t0) and after 4 (t1) and 8 weeks (t2) of regular intake. Skin elasticity (primary interest) was also assessed at follow-up 4 weeks after the last intake of CH (t3, 4-week regression phase). At the end of the study, skin elasticity in both CH dosage groups showed a statistically significant improvement in comparison to placebo. After 4 weeks of follow-up treatment, a statistically significantly higher skin elasticity level was determined in elderly women. With regard to skin moisture and skin evaporation, a positive influence of CH treatment could be observed in a subgroup analysis, but data failed to reach a level of statistical significance. No side effects were noted throughout the study.

  12. Neuroprotection afforded by diazepam against oxygen/glucose deprivation-induced injury in rat cortical brain slices.

    Science.gov (United States)

    Ricci, Lorenzo; Valoti, Massimo; Sgaragli, Giampietro; Frosini, Maria

    2007-04-30

    The aim of the present investigation was to assess neuroprotection exerted by diazepam (0.1-25 microM) in rat cortical brain slices subjected to oxygen-glucose deprivation and reoxygenation. Neuronal injury and neuroprotection were assessed by measuring the release of glutamate and lactate dehydrogenase and tissue water content. Results demonstrate that diazepam exerted neuroprotective effects according to a "U-shaped", hormetic-like, concentration-response curve, with an efficacy window of 0.5-5 microM concentration. Flumazenil (20 microM) fully antagonised neuroprotection afforded by 5 microM diazepam. In conclusion, the hormetic response of diazepam should be taken into consideration when designing experiments aimed at assessing diazepam neuroprotection against ischemia/reoxygenation injury.

  13. Defining hormesis: evaluation of a complex concentration response phenomenon.

    Science.gov (United States)

    Kendig, Eric L; Le, Hoa H; Belcher, Scott M

    2010-01-01

    Hormesis describes dose-response relationships characterized by a reversal of response between low and high doses of chemicals, biological molecules, physical stressors, or other initiators of a response. Acceptance of hormesis as a viable dose-response theory has been limited until recently, in part, because of poor conceptual understanding, ad hoc and inappropriate use, and lack of a defined mechanism. By examining the history of this dose-response theory, it is clear that both pharmacological and toxicological studies provide evidence for hormetic dose responses, but retrospective examination of studies can be problematic at best. Limited scientific evidence and lack of a common lexicon with which to describe these responses have left hormesis open to inappropriate application to unrelated dose-response relationships. Future studies should examine low-dose effects using unbiased, descriptive criteria to further the scientific understanding of this dose response. A clear, concise definition is required to further the limited scientific evidence for hormetic dose responses.

  14. Dose-response features of neuroprotective agents: an integrative summary.

    Science.gov (United States)

    Calabrese, Edward J

    2008-01-01

    This article provides an integrative summary of the effects of neuroprotective agents on neuronal survival and neurite outgrowth using primary cell cultures, multiple neuronal cell lines, and astroglial cells. These findings are dealt with in considerable detail in the following three articles (Calabrese, 2008a, 2008b, 2008c) of this series of issues of Critical Reviews in Toxicology. The principal finding is that the overwhelming majority of neuroprotective agents display biphasic dose responses, characterized by modest low-dose enhancement/stimulation and high-dose inhibitory responses. The quantitative features of these dose responses are consistent with the hormetic dose-response model. Mechanisms that account for numerous hormetic dose responses of neuroprotective agents are summarized, as well as the clinical implications of specific experimental findings.

  15. Vegetable and synthetic tannins induce hormesis/toxicity in sea urchin early development and in algal growth

    Energy Technology Data Exchange (ETDEWEB)

    De Nicola, Elena [Italian National Cancer Institute, G. Pascale Foundation, via M. Semmola, I-80131 Naples (Italy); Meric, Suereyya [Department of Civil Engineering, Salerno University, I-84084 Fisciano (Italy); Gallo, Marialuisa [Campania Regional Agency for Environmental Protection (ARPAC), I-80143 Naples (Italy); Iaccarino, Mario [Italian National Cancer Institute, G. Pascale Foundation, via M. Semmola, I-80131 Naples (Italy); Della Rocca, Claudio [Department of Civil Engineering, Salerno University, I-84084 Fisciano (Italy); Lofrano, Giusy [Department of Civil Engineering, Salerno University, I-84084 Fisciano (Italy); Russo, Teresa [Campania Regional Agency for Environmental Protection (ARPAC), I-80143 Naples (Italy); Pagano, Giovanni [Italian National Cancer Institute, G. Pascale Foundation, via M. Semmola, I-80131 Naples (Italy)]. E-mail: gbpagano@tin.it

    2007-03-15

    Mimosa tannin and phenol-based synthetic tannin (syntan) were tested for toxicity to sea urchin (Paracentrotus lividus and Sphaerechinus granularis) early development and to marine algal growth (Dunaliella tertiolecta). Sea urchin embryogenesis was affected by vegetable tannin and syntan water extracts (VTWE and STWE) at levels {>=}1 mg/L. Developmental defects were significantly decreased at VTWE and STWE levels of 0.1 and 0.3 mg/L when control cultures displayed suboptimal quality, i.e. <70% 'viable' (normal or retarded) larvae. Fertilization success of sea urchin sperm was increased up to 0.3 mg/L STWE or VTWE, then was inhibited by increasing tannin levels (1-30 mg/L). Offspring abnormalities, following sperm exposure to VTWE or STWE, showed the same shift from hormesis to toxicity. Cell growth bioassays in D. tertiolecta exposed to VTWE or STWE (0.1-30 mg/L) showed non-linear concentration-related toxicity. Novel criteria are suggested in defining control quality that should reveal hormetic effects. - Vegetable tannin and synthetic tannins were moderately toxic or displayed hormetic effects in sea urchins and in algae. Re-defining control quality is needed for evaluating hormetic effects.

  16. Effects of IMOD™ and Angipars™ on mouse D-galactose-induced model of aging.

    Science.gov (United States)

    Ghanbari, Samane; Yonessi, Mahsa; Mohammadirad, Azadeh; Gholami, Mahdi; Baeeri, Maryam; Khorram-Khorshid, Hamid Reza; Gharibdoost, Farhad; Abdollahi, Mohammad

    2012-10-27

    The aim of this study was to evaluate the effects of two registered herbal drugs called IMOD and Angipars on mouse model. Aging was induced by D-galactose (500 mg/kg) administered to animals for 6 weeks through drinking water. Male BALB/c mice were randomly divided into 5 groups receiving D-galactose (D-galactose, 500 mg/kg) for 6 weeks; positive control (D-galactose [500 mg/kg] for 6 weeks + Vitamin E [200 mg/kg/day] intraperitoneally for 4 weeks); IMOD (D-galactose [500 mg/kg] for 6 weeks + IMOD [20 mg/kg/day] intraperitoneally for 4 weeks), Angipars (D-galactose [500 mg/kg] for 6 weeks + Angipars [2.1 mg/kg/day] by gavage for 4 weeks); and the fifth group that was sham and not given D-galactose. At the end of treatment, pro-inflammatory markers including tumor necrosis factor-α (TNF-α), interlukine-1β (IL-β), interlukine-6 (IL-6), Nuclear Factor-kappaB (NF-κb), total antioxidant power (TAP), lipid peroxides (LPO) and male sex hormones i.e. testosterone and dehydroepiandrosterone-sulfate (DHEA-S) were measured in the blood.Results showed that D-Galactose induces a significant oxidative stress and proinflammatory cascade of aging while both IMOD and Angipars recovered all of them. Interestingly, IMOD and Angipars were better than Vitamin E in improving male sex hormones in aged mice. This effect is so important and should be considered as an advantage although it cannot be explained with current knowledge. The conclusion is that IMOD and Angipars have marked anti-aging effect on D-galactose-induced model of aging.

  17. Effects of IMOD™ and Angipars™ on mouse D-galactose-induced model of aging

    Directory of Open Access Journals (Sweden)

    Ghanbari Samane

    2012-10-01

    Full Text Available Abstract The aim of this study was to evaluate the effects of two registered herbal drugs called IMOD and Angipars on mouse model of. Aging was induced by D-galactose (500 mg/kg administered to animals for 6 weeks through drinking water. Male BALB/c mice were randomly divided into 5 groups receiving D-galactose (D-galactose, 500 mg/kg for 6 weeks; positive control (D-galactose [500 mg/kg] for 6 weeks + Vitamin E [200 mg/kg/day] intraperitoneally for 4 weeks; IMOD (D-galactose [500 mg/kg] for 6 weeks + IMOD [20 mg/kg/day] intraperitoneally for 4 weeks, Angipars (D-galactose [500 mg/kg] for 6 weeks + Angipars [2.1 mg/kg/day] by gavage for 4 weeks; and the fifth group that was sham and not given D-galactose. At the end of treatment, pro-inflammatory markers including tumor necrosis factor-α (TNF-α, interlukine-1β (IL-β, interlukine-6 (IL-6, Nuclear Factor-kappaB (NF-κb, total antioxidant power (TAP, lipid peroxides (LPO and male sex hormones i.e. testosterone and dehydroepiandrosterone-sulfate (DHEA-S were measured in the blood. Results showed that D-Galactose induces a significant oxidative stress and proinflammatory cascade of aging while both IMOD and Angipars recovered all of them. Interestingly, IMOD and Angipars were better than Vitamin E in improving male sex hormones in aged mice. This effect is so important and should be considered as an advantage although it cannot be explained with current knowledge. The conclusion is that IMOD and Angipars have marked anti-aging effect on D-galactose-induced model of aging.

  18. Effects of IMOD and Angipars on Mouse D-Galactose-Induced Model of Aging

    Directory of Open Access Journals (Sweden)

    Samane Ghanbari

    2012-10-01

    Full Text Available The aim of this study was to evaluate the effects of two registered herbal drugs called IMOD and Angipars on mouse model of. Aging was induced by D-galactose (500 mg/kgadministered to animals for 6 weeks through drinking water. Male BALB/c mice were randomly divided into 5 groups receiving D-galactose (D-galactose, 500 mg/kg for 6 weeks; positive control (D-galactose [500 mg/kg] for 6 weeks + Vitamin E [200 mg/kg/day]intraperitoneally for 4 weeks; IMOD (D-galactose [500 mg/kg] for 6 weeks + IMOD [20 mg/kg/day] intraperitoneally for 4 weeks, Angipars (D-galactose [500 mg/kg] for 6 weeks + Angipars [2.1 mg/kg/day] by gavage for 4 weeks; and the fifth group that was sham and not given D-galactose. At the end of treatment, pro-inflammatory markers including tumor necrosis factor-α (TNF-α, interlukine-1β (IL-β, interlukine-6 (IL-6, NF-kappaB (NF-κb, total antioxidant power (TAP, lipid peroxides (LPO and male sex hormones i.e.testosterone and dehydroepiandrosterone-sulfate (DHEA-S were measured in the blood.Results showed that D-Galactose induces a significant oxidative stress and proinflammatory cascade of aging while both IMOD and Angipars recovered all of them. Interestingly, IMOD and Angipars were better than Vitamin E in improving male sex hormones that were declined in aged mice. This effect is so important and should be considered as an advantage although it cannot be explained with current knowledge. The conclusion is that IMOD and Angipars have marked anti-aging effect on D-galactose-induced model of aging.

  19. Klotho expression is reduced in COPD airway epithelial cells: effects on inflammation and oxidant injury.

    Science.gov (United States)

    Gao, Wei; Yuan, Cheng; Zhang, Jingying; Li, Lingling; Yu, Like; Wiegman, Coen H; Barnes, Peter J; Adcock, Ian M; Huang, Mao; Yao, Xin

    2015-12-01

    COPD (chronic obstructive pulmonary disease) is associated with sustained inflammation, excessive injury, and accelerated lung aging. Human Klotho (KL) is an anti-aging protein that protects cells against inflammation and damage. In the present study, we quantified KL expression in the lungs of COPD patients and in an ozone-induced mouse model of COPD, and investigated the mechanisms that control KL expression and function in the airways. KL distribution and levels in human and mouse airways were measured by immunohistochemistry and Western blotting. The effect of CSE (cigarette smoke extract) on KL expression was detected in human bronchial epithelial cells. Moreover, the effect of KL on CSE-mediated inflammation and hydrogen peroxide-induced cellular injury/apoptosis was determined using siRNAs. KL expression was decreased in the lungs of smokers and further reduced in patients with COPD. Similarly, 6 weeks of exposure to ozone decreased KL levels in airway epithelial cells. CSE and TNFα (tumour necrosis factor α) decreased KL expression and release from airway epithelial cells, which was associated with enhanced pro-inflammatory cytokine expression. Moreover, KL depletion increased cell sensitivity to cigarette smoke-induced inflammation and oxidative stress-induced cell damage. These effects involved the NF-κB (nuclear factor κB), MAPK (mitogen-activated protein kinase) and Nrf2 (nuclear factor erythroid 2-related factor 2) pathways. Reduced KL expression in COPD airway epithelial cells was associated with increased oxidative stress, inflammation and apoptosis. These data provide new insights into the mechanisms associated with the accelerated lung aging in COPD development.

  20. Neuronal in vitro activity is more sensitive to valproate than intracellular ATP: Considerations on conversion problems of IC50 in vitro data for animal replacement.

    Science.gov (United States)

    Nissen, Matthias; Buehler, Sebastian M; Stubbe, Marco; Gimsa, Jan

    2016-06-01

    We investigated the effects of acute valproate (VPA) on mouse embryonic primary cortex cells (MEPCs). Intracellular ATP concentrations were compared with changes in the mean action potential (AP) frequencies of MEPC networks growing on microelectrode arrays. Our data implies biphasic reactions towards increasing VPA concentrations for both parameters. Intracellular ATP and mean AP frequencies increased around characteristic concentrations of 0.15 and 0.07mM to hormetic plateaus of approx. 120% and 160% of their controls, before fading around 17 and 1.7 mM, respectively. The biphasic in vitro behavior of the two parameters hinders a simple extraction of IC50 and Hillslope values. Different ways of data-fitting with single and double logistic functions are discussed. For a typical hormetic increase of 60% above control, IC50 and Hillslope were decreased by 37% and 15%, respectively. Despite these marginal effects at a logarithmic concentration scale, the hormetic and double logistic behavior of parameters may provide information on the mode of action of toxic compounds. Comparison of our values with the LD50 of mice, recalculated by normalization to body mass, suggests that a neurotoxic rather than a cytotoxic mechanism is killing the animals. The future use of cellular microsystems to replace animal experiments will motivate the development of new microsensors, as well as the consideration of newly accessible parameters in systems biology models.

  1. In vivo photoprotective effects of cosmetic formulations containing UV filters, vitamins, Ginkgo biloba and red algae extracts.

    Science.gov (United States)

    Mercurio, D G; Wagemaker, T A L; Alves, V M; Benevenuto, C G; Gaspar, L R; Maia Campos, P M B G

    2015-12-01

    an interesting combination for an effective photoprotective formulation with anti-aging properties.

  2. Depigmentation and rejuvenation effects of kinetin on the aged skin of hairless descendants of Mexican hairless dogs.

    Science.gov (United States)

    Kimura, Tohru; Doi, Kunio

    2004-01-01

    The depigmenting and anti-aging effects of kinetin (KN) solutions on the aged skin of hairless dogs were clinically and histologically investigated. Grossly, all KN-treated sites became mildly depigmented. At 50 days of topical treatment with KN solutions, apparent improvement was observed in the skin texture, wrinkling, and pigmentation. At 100 days of KN treatment, both the skin rejuvenation and depigmentation effects became more prominent. Throughout the experimental period of KN treatment, no adverse effects were found in any sites treated with KN solutions. In the colorimetric system, at 100 days of topical treatment with KN solutions, the L* and b* values in the sites treated with KN solutions significantly increased. Histologically, at 50 days of topical treatment with KN solutions, the KN-treated sites showed a decrease in the thickness of the corneal layers. Melanin granules decreased throughout all epidermal layers. In the dermis, the large number of fine collagen and elastic fibers were densely aligned. At the end of the treatment, this agent was equally effective against pigmented lesions irrespective of the concentration of KN solution. The distribution of melanin granules returned to normal in the skin of adult hairless dogs. Throughout the present study, there were no histologic abnormalities in the epidermis and the dermis. These results revealed that topical treatment with lower concentrations of KN solutions normalized hyperpigmentation and improved the aged skin structure of hairless dogs. In addition, it was clarified that KN solutions had no adverse effects on the skin of hairless dogs and that this agent was a safe chemical for long-term application.

  3. The Effect of a Phaseolus vulgaris and Dietary Fiber Based Supplement on Advanced Glycation End Products: An Open-label Trial

    Directory of Open Access Journals (Sweden)

    Brett J. West

    2015-07-01

    Full Text Available Elevated Advanced Glycation End product (AGE levels are associated with certain impaired health states. As these are disruptive to the function of healthy tissues, due to their protein cross-linking ability, AGEs are significant contributors to the aging process. In fact, population studies have revealed that AGE levels tend to increase as we get older. Certain lifestyle and dietary factors may accelerate AGE accumulation. Therefore, strategies intended to modify these factors, or mitigate their effects, may be useful in controlling the aging process. In an 11 week open-label clinical trial, 30 adult volunteers consumed daily a commercially available combination of white kidney bean extract, dietary fibers, &beta-carotene and noni (Morinda citrifolia fruit pulp, in combination with calorie restriction and exercise. During the course of the trial, participants experienced significant weekly declines in average body weight and fat mass. The average AGE score, as measured by skin auto-fluorescence, had also decreased significantly. In terms of AGE associated years, the change in AGE scores corresponded to an average decrease of 8.83 years. The results indicate that the intervention contributed to improved health and exhibited anti-aging properties.

  4. Effects of chronic calorie restriction or dietary resveratrol supplementation on insulin sensitivity markers in a primate, Microcebus murinus.

    Directory of Open Access Journals (Sweden)

    Julia Marchal

    Full Text Available The prevalence of diabetes and hyperinsulinemia increases with age, inducing metabolic failure and limiting lifespan. Calorie restriction (CR without malnutrition delays the aging process, but its long-term application to humans seems difficult. Resveratrol (RSV, a dietary polyphenol, appears to be a promising CR mimetic that can be easily administered in humans. In this work, we hypothesized that both CR and RSV impact insulin sensitivity in a non-human primate compared to standard-fed control (CTL animals. Four- to five-year-old male grey mouse lemurs (Microcebus murinus were assigned to three dietary groups: a CTL group, a CR group receiving 30% fewer calories than the CTL and a RSV group receiving the CTL diet supplemented with RSV (200 mg·day(-1·kg(-1. Insulin sensitivity and glycemia were assessed using an oral glucose tolerance test (OGTT and the homeostasis model assessment of insulin resistance (HOMA-IR index evaluation after 21 or 33 months of chronic treatment. Resting metabolic rate was also measured to assess the potential relationships between this energy expenditure parameter and insulin sensitivity markers. No differences were found after a 21-month period of treatment, except for lower glucose levels 30 min after glucose loading in CR animals. After 33 months, CR and RSV decreased glycemia after the oral glucose loading without decreasing fasting blood insulin. A general effect of treatment was observed on the HOMA-IR index, with an 81% reduction in CR animals and 53% in RSV animals after 33 months of treatment compared to CTL. Chronic CR and dietary supplementation with RSV affected insulin sensitivity by improving the glucose tolerance of animals without disturbing their baseline insulin secretion. These results suggest that both CR and RSV have beneficial effects on metabolic alterations, although these effects are different in amplitude between the two anti-aging treatments and potentially rely on different metabolic

  5. Influence of carbonyl stress on rheological alterations of blood materials and decarbonylation effect of glutathione

    Institute of Scientific and Technical Information of China (English)

    彭密军; 蔡建光; 贺洪; 龚萍; 李国林; 汤婷; 朱泽瑞; 印大中

    2008-01-01

    The effects of various toxic carbonyls such as malondialdehyde(MDA),a secondary product of lipid peroxidation,and other aldehydes on rheological parameters and their relationship with aging-associated alterations were studied.Both MDA and glutaraldehyde(Glu) in different concentrations significantly increase viscosity,plastic viscosity and yield stress of human plasma and erythrocyte suspensions.MDA(20 mmol/L) reduces sharply the typical fluorescence of proteins(excitation 280 nm/emission 350 nm),and produces age pigment-like fluorescence with a strong emission peak at 460 nm when excites at 395 nm by only being incubated for some hours.In contrast,Glu decreases merely the fluorescence of proteins without producing age pigment-like fluorescence.These data suggest interestingly that the MDA-induced gradual protein cross linking seems to form from different mechanisms compared to the fast rheological changes of blood materials which may take place either in acute and chronic diseases or during aging.On the other hand,MDA induces various deleterious alterations of erythrocytes whereas glutathione(GSH) inhibits the MDA-related carbonyl stress in a concentration-dependent manner.The results indicate that carbonyl-amino reaction exists in the blood widely and GSH has the ability to interrupt or reverse this reaction in a certain way.It implies that carbonyl stress may be one of the important factors in blood stasis and suggests a theoretical and practical approach in anti-stresses and anti-aging.

  6. 中药皂苷的植物雌激素样作用%Phytoestrogen-like effects of herbal saponins

    Institute of Scientific and Technical Information of China (English)

    徐先祥; 吴杨峥; 孙爱静; 刁勇

    2011-01-01

    Phytoestrogens (Pes) are defined as the plant compounds with structures or activities similarly to estrogen. Benefits of Pes to human health get universal attentions. Multifarious herbal components play the roles as phy-toestrogen. Saponins, important natural products, exhibit remarkable pharmacological effects including anti-tumor, anti-oxidant, anti-inflammation, immunoregulation, anti-osteoporosis, antivirus, cardiovascular protection, neuropro-tection, anti-aging and regulation of glucose andlipid metabolism. Saponins are clinically valuable for applications in hormone-related diseases like endometriosis, breast cancer, prostate cancer, osteoporosis and cardiovascular diseases. It is theoretically and practically significant to further elucidate phytoestrogen-like effects of saponins.%植物雌激素是指具有类似动物雌激素结构或活性的植物成分,其对健康的重要作用受到广泛关注,中药含有多种成分具有植物雌激素作用.皂苷是一类重要的天然产物,具有抗肿瘤、抗氧化、抗炎和免疫调节、抗骨质疏松、抗病毒、心血管保护、神经保护、延缓衰老、调节糖脂代谢等作用,临床上对于子宫内膜异位症、乳腺癌、前列腺癌、骨质疏松、心血管疾病等激素相关疾病具有重要应用价值,进一步阐发皂苷的植物雌激素样作用具有重要理论意义和实践价值.

  7. Protective Effect of Garlic on Cellular Senescence in UVB-Exposed HaCaT Human Keratinocytes.

    Science.gov (United States)

    Kim, Hye Kyung

    2016-07-29

    Ultraviolet (UV) irradiation generates reactive oxygen species (ROS) in the cells, which induces the cellular senescence and photoaging. The present study investigated the protective effects of garlic on photo-damage and cellular senescence in UVB-exposed human keratinocytes, HaCaT cells. An in vitro cell free system was used to examine the scavenging activity of 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radicals and nitric oxide (NO). The effect of garlic extract on ROS formation, MMP-1 protein and mRNA expressions, cytokines such as interleukin (IL)-1β and IL-6, senescence associated-β-galactosidase (SA-β-gal) activity, and silent information regulator T1 (SIRT1) activity were determined in UVB-irradiated HaCaT cells. Garlic exhibited strong DPPH radical and NO scavenging activity in cell free system exhibiting IC50 values of 2.50 mg/mL and 4.38 mg/mL, respectively. Garlic pretreatment attenuated the production of UVB-induced intracellular ROS. MMP-1 level, which has been known to be induced by ROS, was dramatically elevated by UVB irradiation, and UVB-induced MMP-1 mRNA and protein expressions were significantly reduced by garlic treatment (50 µg/mL) comparable to those of UV-unexposed control cells. UV-induced pro-inflammatory cytokine productions (IL-6 and IL-1β) were significantly inhibited by pretreatment with garlic in a dose-dependent manner. SA-β-gal activity, a classical biomarker of cellular senescence, and SIRT1 activity, which has attracted attention as an anti-aging factor in recent years, were ameliorated by garlic treatment in UV-irradiated HaCaT cells. The present study provides the first evidence of garlic inhibiting UVB-induced photoaging as a result of augmentation of cellular senescence in HaCaT human keratinocytes.

  8. Lifestyle-induced metabolic inflexibility and accelerated ageing syndrome: insulin resistance, friend or foe?

    Directory of Open Access Journals (Sweden)

    Bell Jimmy D

    2009-04-01

    Full Text Available Abstract The metabolic syndrome may have its origins in thriftiness, insulin resistance and one of the most ancient of all signalling systems, redox. Thriftiness results from an evolutionarily-driven propensity to minimise energy expenditure. This has to be balanced with the need to resist the oxidative stress from cellular signalling and pathogen resistance, giving rise to something we call 'redox-thriftiness'. This is based on the notion that mitochondria may be able to both amplify membrane-derived redox growth signals as well as negatively regulate them, resulting in an increased ATP/ROS ratio. We suggest that 'redox-thriftiness' leads to insulin resistance, which has the effect of both protecting the individual cell from excessive growth/inflammatory stress, while ensuring energy is channelled to the brain, the immune system, and for storage. We also suggest that fine tuning of redox-thriftiness is achieved by hormetic (mild stress signals that stimulate mitochondrial biogenesis and resistance to oxidative stress, which improves metabolic flexibility. However, in a non-hormetic environment with excessive calories, the protective nature of this system may lead to escalating insulin resistance and rising oxidative stress due to metabolic inflexibility and mitochondrial overload. Thus, the mitochondrially-associated resistance to oxidative stress (and metabolic flexibility may determine insulin resistance. Genetically and environmentally determined mitochondrial function may define a 'tipping point' where protective insulin resistance tips over to inflammatory insulin resistance. Many hormetic factors may induce mild mitochondrial stress and biogenesis, including exercise, fasting, temperature extremes, unsaturated fats, polyphenols, alcohol, and even metformin and statins. Without hormesis, a proposed redox-thriftiness tipping point might lead to a feed forward insulin resistance cycle in the presence of excess calories. We therefore suggest

  9. Effect of Modified Red Pottery Clay on the Moisture Absorption Behavior and Weatherability of Polyethylene-Based Wood-Plastic Composites

    Directory of Open Access Journals (Sweden)

    Qingde Li

    2017-01-01

    Full Text Available Red pottery clay (RPC was modified using a silane coupling agent, and the modified RPC (mRPC was then used to enhance the performance of high-density polyethylene-based wood-plastic composites. The effect of the mRPC content on the performances of the composites was investigated through Fourier transform infrared spectrometry, differential mechanical analysis (DMA and ultraviolet (UV-accelerated aging tests. After adding the mRPC, a moisture adsorption hysteresis was observed. The DMA results indicated that the mRPC effectively enhanced the rigidity and elasticity of the composites. The mRPC affected the thermal gravimetric, leading to a reduction of the thermal degradation rate and a right-shift of the thermal degradation peak; the initial thermal degradation temperature was increased. After 3000 h of UV-accelerated aging, the flexural strength and impact strength both declined. For aging time between 0 and 1000 h, the increase in amplitude of ΔL* (luminescence and ΔE* (color reached a maximum; the surface fading did not became obvious. ΔL* and ΔE* increased more significantly between 1000 and 2000 h. These characterization results indicate that the chromophores of the mRPC became briefly active. However, when the aging times were higher than 2000 h, the photo-degradation reaction was effectively prevented by adding the mRPC. The best overall enhancement was observed for an mRPC mass percentage of 5%, with a storage modulus of 3264 MPa and an increase in loss modulus by 16.8%, the best anti-aging performance and the lowest degree of color fading.

  10. Cosmeceutical Effects of Galactomannan Fraction from Arenga pinnata Fruits In vitro

    Science.gov (United States)

    Yanti; Madriena; Ali, Soegianto

    2017-01-01

    Background: Cosmeceuticals refer to natural cosmetics with medical-like benefits due to their bioactive contents. Sugar palm fruit (Arenga pinnata) extract has been claimed for its anti-aging effect in vitro. However, its active compounds for cosmeceuticals is still unclear. Objective: This study was aimed to extract galactomannan from A. pinnata fruits and test its efficacy for tyrosinase inhibition, antioxidant, and anti-photoaging activities in vitro. Materials and Methods: Galactomannan from A. pinnata fruits was extracted by freeze drying and identified for its chemical compounds by using pyrolysis gas chromatography-mass spectrometry (py-GC/MS). Galactomannan was tested for its tyrosinase inhibition in both cell-based (melanocytes) and enzymatic assays, antioxidant activity using ferrous ion chelating assay (FCA) assay, and anti-photoaging activity for inhibiting the gene expression of matrix metalloproteinase-1 (MMP-1) and MMP-13 in macrophages using quantitative real-time polymerase chain reaction (qRT-PCR) analysis. Results: Identification of galactomannan fraction from A. pinnata fruits by py-GC/MS mainly consisted of oxonium ion and glucosides. For cellular assay, galactomannan at 5 μg/mL inhibited >50% of tyrosinase activity in melanocytes induced by phorbol myristate acetate. At the enzymatic level, galactomannan at similar concentration showed less tyrosinase activity inhibition (~20%). FCA results showed that galactomannan at 10 μg/mL exerted >50% of antioxidant activity. The qRT-PCR data indicated that galactomannan at 5 μg/mL inhibited >50% of MMP-1 and MMP-13 gene expressions in ultraviolet B-treated macrophages. Conclusion: Galactomannan fraction from A. pinnata fruits has efficacy for enlightening effect, antioxidant, and anti-photoaging activity in the dose-independent pattern, indicating its cosmeceutical effects for skin healthcare. SUMMARY A. pinnata fruit containing galactomannan has cosmeceutical potentials through enlightening effect

  11. Blood pressure-independent effect of candesartan on cardio-ankle vascular index in hypertensive patients with metabolic syndrome

    Directory of Open Access Journals (Sweden)

    Kanako Bokuda

    2010-07-01

    Full Text Available Kanako Bokuda1, Atsuhiro Ichihara1,2, Mariyo Sakoda1, Asako Mito1, Kenichiro Kinouchi1, Hiroshi Itoh11Department of Internal Medicine, Keio University School of Medicine, Tokyo, Japan; 2Department of Endocrinology and Anti-Aging Medicine, Keio University School of Medicine, Tokyo, JapanAbstract: Angiotensin receptor blockers (ARBs are known to reduce the cardiovascular risk in hypertensive patients. This study was designed to examine the effect of an ARB candesartan on subclinical atherosclerosis assessed by cardio-ankle vascular index (CAVI in comparison with calcium channel blockers (CCBs alone in hypertensive patients with metabolic syndrome (MetS. A total of 53 consecutive hypertensive patients with MetS were randomly assigned to the candesartan group, in which candesartan was added on, or the CCBs group, in which CCBs were added on. Clinical and biological parameters were obtained before and after the 12-month treatment period. The primary measure of efficacy was the %change in CAVI. When treated with candesartan, but not CCBs, CAVI significantly decreased from 8.7 to 7.7 by 11%. Blood pressure (BP significantly decreased with both treatments, but the differences between groups were not significant. The changes in other parameters remained unchanged in both the groups. Analysis of covariance found that both the BP reduction and the therapy difference contributed to the decrease in CAVI, but the BP reduction was not involved in the decrease in CAVI caused by the difference in the therapy. Candesartan may be a better antihypertensive drug than CCBs to that subclinical atherosclerosis of patients with MetS.Keywords: albuminuria, ambulatory blood pressure, calcium channel blockers, carotid ­intima-media thickness

  12. Research Progress about Pharmacological Effect and Mechanism of Flavonoids in Traditional Chinese Medicine%中药黄酮类化合物药理作用及作用机制研究进展

    Institute of Scientific and Technical Information of China (English)

    马锐; 吴胜本

    2013-01-01

    目的 对黄酮类化合物的药理作用及其作用机制进行综述和分析.方法 对近年来有关黄酮类化合物的药理作用及其作用机制的文献进行总结与分析.结果 黄酮类化合物具有抗氧化、抗炎、镇痛、调节免疫、抗衰老、降血脂、抗肿瘤等药理作用,其作用机制可能与其抗自由基或抗氧化有关.结论 黄酮类化合物是许多中草药的有效成分,具有广泛的生物活性,其产生生物活性的作用机制有待于进一步深入的研究.%Objective To review and analyze the pharmacological effect and mechanism of flavonoids in traditional Chinese medicine. Methods The related references in recent years home and abroad about the pharmacological effect and mechanism of flavonoids in traditional Chinese medicine were summarized and analyzed. Results Flavonoids had a wide range of pharmacological effects such as antioxidant, anti-inflammatory and analgesic, regulating immunity, anti-aging, fall hematic fat, antitumor and pharmacological action and so on, and the mechanism of pharmacological effects may be related with the resistance to free radicals or antioxidant. Conclusion The flavonoid compounds were the effective composition of many Chinese herbal medicine, and had a wide range of biological activity, and its biological activity mechanism needed further in-depth research.

  13. Effects of human umbilical cord mesenchymal stem cell transplantation on expression of aging related gene in brain and liver of Alzheimer’s disease mice%人脐带间充质干细胞移植对老年痴呆小鼠脑、肝组织中衰老相关基因表达的影响

    Institute of Scientific and Technical Information of China (English)

    崔渊博; 马珊珊; 姚宁; 渠瑞娜; 王欣欣; 邢衢; 孟楠; 杨波; 关方霞

    2015-01-01

    Aim: To research the anti-aging effects of human umbilical cord mesenchymal stem cells ( hUC-MSCs ) transplantation on transgenic APP +mice and to investigate the anti-aging mechanisms in terms of age-related genes. Meth-ods:APP+transgenic mice were identified by PCR. hUC-MSCs were isolated in vitro sterilely. At the third passage , sin-gle cell suspension at 1 ×106 mL-1 was obtained and transplanted by tail venous pathway in the transplantation group. The anti-aging effects on transgenic APP +mice were detected by Morris water maze test at three weeks after transplantation to study the learning and memory ability of the mice. qRT-PCR and Western blot were performed to test the change of mRNA and protein expression of age-related gene in the brain and liver of the APP +mouse, including p16, p21, p53, silence in-formation regulator 2(Sirt2), and proliferating cell nuclear antigen (PCNA).Results:12 APP+mice were identified total-ly.Compared with control group, the escaping latency was significantly shorter (P<0.05), the number of crossing plat-form quadrant increased and the time of crossing platform quadrant was significantly longer in the transplantation group (P<0.05).What’s more, the expressions of Sirt2 and PCNA were increased, and p21, p53 but not p16 decreased in the brain and liver tissue of transplanted APP +mice(P<0.05).Conclusion:The learning and memory ability and anti-aging effect are increased significantly in APP +mice after hUC-MSCs tansplantation. The underlying mechanism is partly due to the change of aging related gene such as p 21, p53, PCNA and Sirt2, which may interact with each other and constitute the gene regulation chain of cell aging.%目的:研究人脐带间充质干细胞(hUC-MSCs)静脉移植对APP+转基因鼠衰老的调控作用,并从衰老相关基因角度探讨MSCs延缓衰老的机制。方法:采用PCR技术对老年痴呆APP+转基因鼠进行分子鉴定;将传至第3代的hUC-MSCs细胞密度调整为1

  14. Effect of botanicals on inflammation and skin aging: analyzing the evidence.

    Science.gov (United States)

    Suggs, Amanda; Oyetakin-White, Patricia; Baron, Elma D

    2014-01-01

    The skin and its immune system manifest a decline in physiologic function as it undergoes aging. External insults such as ultraviolet light exposure cause inflammation, which may enhance skin aging even further leading to cancer and signs of photoaging. There is a potential role for botanicals as an adjunct modality in the prevention of skin aging. Numerous over-the-counter anti-aging products are commercially available, many of which boast unverified claims to reduce stress, inflammation and correct signs of aging. In this article we reviewed the scientific literature for data on frequently published "anti-inflammaging" additives such as vitamins A, C and E and green tea. We also analyzed the evidence available on five promising ingredients commonly found in anti-aging products, namely, argan oil, rosemary, pomegranate, Coenzyme Q10, and Coffeeberry. Though there may be an increasing amount of scientific data on a few of these novel botanicals, in general, there remains a lack of clinical data to support the anti-aging claims made.

  15. 睡眠剥夺对大鼠抗氧化应激的影响及中药的干预作用%The Effect of Anti-oxidation in Rats Induced by Sleep Deprivation and Altered by Traditional Chinese Medicine

    Institute of Scientific and Technical Information of China (English)

    徐孝平; 潘永明; 冷晓霞; 朱科燕; 寿旗扬; 周卫民; 贾临超; 陈民利

    2011-01-01

    [目的]观察睡眠剥夺对大鼠饮食行为、能量消耗、体温和杭氧化能力的影响,探讨中药的干预作用及其抗氧化应激的机制.[方法]将雌性SD大鼠32只随机分为4组,即正常对照组、模型对照组、抗衰老片组、六味地黄丸组,每组8只,利用"小平台水环境法"建立大鼠睡眠剥夺模型,分别进行体温、体重、进食量和饮水量的监测,并计算日能耗,同时检测机体血清SOD活性和MDA含量.[结果]72h睡眠剥夺后,模型对照组大鼠体重、进食量和饮水量均明显低于正常对照组(P<0.05,P<0.01),日消耗能量和体温明显高于正常对照组(P<0.05,P<0.01),且机体血清SOD活性明显降低(P<0.01),MDA含量明显升高(P<0.01);进行抗衰老片和六味地黄丸干预后,均能有效预防睡眠剥夺后大鼠的体重、进食量、饮水量的降低,并能降低日消耗能量和体温,显著提高机体SOD活性(P<0.05,p<0.01),降低MDA含量(P<0.05,P<0.01).[结论]睡眠剥夺可引起大鼠饮食行为的改变、耗能增加、体温升高,抗氧化应激能力下降.而抗衰老片和六味地黄丸等中药具有抗睡眠剥夺引起的氧化应激的作用,其机制可能与提高机体的抗氧化能力有关.%[Objective]To explore the effect of sleep deprivation on oxidative stress in rats, moreover, the effect of Anti-aging tablets was observed in rats of sleep deprivation. [Methods]A total of 32 female SD rats were randomly divided into four groups: normal control group, model control group, Anti-aging tablets and Liuwei Dihuang Pills group,8 rats per group. The "flower pot" technique was used to establish rats sleep deprivation model. The body temperature, weight, intake food, drinking amount of water,daily consumption of energy, serum of SOD activity and MDA content of all rats were measured respectively. [Results]72h after sleep deprivation, compared with normal control group,the weight, intake food,and drinking amount of

  16. Effects of Longyanshen polysaccharides on free radical metabolism in senescence accelerated-prone mice

    Institute of Scientific and Technical Information of China (English)

    Zhongshi Huang; Haiyuan Xie; Shijun Zhang; Yang Jiao; Weizhe Jiang; Renbin Huang

    2008-01-01

    BACKGROUND: Along with aging, antioxidase activity decreases and oxygen-derived free radicals greatly accumulate, resulting in cellular senescence, or even cell death. This is manifested by hypomnesia and disordered metabolism of free radicals. Studies have reported that Longyanshen polysaccharidcs have the function of antioxidation and improved brain memory.OBJECTIVE: To observe the effects of Longyanshen polysaccharides on free radical metabolism in brain tissue to verify the anti-aging mechanisms in senescence accelerated-prime (SAMPS) mice. DESIGN, TIME AND SETTING: The randomized, controlled, biochemical experiment was performed in the Department of Pharmacology and Scientific Experimental Center of Guangxi Medical University (China) from September 2005 to January 2008.MATERIALS: Forty SAMP8 mice were randomized into four groups: SAMP8 control group, as well as low-, mid-, and high-dose polysaccharide, with 10 mice in each group. Ten senescence accelerated-resistant-prone (SAMR 1) mice served as the normal control group. Longyanshen polysaccharides, extracted from the medical plant Longyanshen, were supplied by the Department of Pharmacology, Guangxi Medical University. Superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), malonaldehydc (MDA), nitric oxide (NO), and total protein test kitwere purchased from Nanjing Jiancheng Bioengineering Institute (China).METHODS: SAMP8 mice were used to establish a dementia animal model. SAMP8 and SAMRI control mice were administered 30 mL/kg saline. The low-, middle-, and high-dose polysaccharide groups were administered 45, 90, and 180 mg/kg Longyanshen polysaccharides, respectively. Each group was treated by intragastric administration, once daily, for 50 continuous days.MAIN OUTCOME MEASURES: One hour after the last administration, mouse brain tissues were collected, and retro orbital blood sampling was performed. Spectrophotometry was used to measure SOD and GSH-Px activity, as well as MDA and NO concentration

  17. Anti-inflammatory effects of cordycepin in lipopolysaccharide-stimulated RAW 264.7 macrophages through Toll-like receptor 4-mediated suppression of mitogen-activated protein kinases and NF-κB signaling pathways

    Directory of Open Access Journals (Sweden)

    Choi YH

    2014-10-01

    Full Text Available Yung Hyun Choi,1,2 Gi-Young Kim,3 Hye Hyeon Lee4 1Department of Biochemistry, Dongeui University College of Korean Medicine, Busan, 2Anti-Aging Research Center and Blue-Bio Industry RIC, Dongeui University, Busan, 3Laboratory of Immunobiology, Department of Marine Life Sciences, Jeju National University, Jeju, 4Daegu Gyeongbuk Institute of Science and Technology, Daegu, Republic of Korea Abstract: Cordycepin is the main functional component of the Cordyceps species, which has been widely used in traditional Oriental medicine. This compound possesses many pharmacological properties, such as an ability to enhance immune function, as well as antioxidant, antiaging, and anticancer effects. In the present study, we investigated the anti-inflammatory effects of cordycepin using a murine macrophage RAW 264.7 cell model. Our data demonstrated that cordycepin suppressed production of proinflammatory mediators such as nitric oxide (NO and prostaglandin E2 by inhibiting inducible NO synthase and cyclooxygenase-2 gene expression. Cordycepin also inhibited the release of proinflammatory cytokines, including tumor necrosis factor-alpha and interleukin-1-beta, through downregulation of respective mRNA expression. In addition, pretreatment with cordycepin significantly inhibited lipopolysaccharide (LPS-induced phosphorylation of mitogen-activating protein kinases and attenuated nuclear translocation of NF-κB by LPS, which was associated with abrogation of inhibitor kappa B-alpha degradation. Furthermore, cordycepin potently inhibited the binding of LPS to macrophages and LPS-induced Toll-like receptor 4 and myeloid differentiation factor 88 expression. Taken together, the results suggest that the inhibitory effects of cordycepin on LPS-stimulated inflammatory responses in RAW 264.7 macrophages are associated with suppression of mitogen-activating protein kinases and activation of NF-κB by inhibition of the Toll-like receptor 4 signaling pathway. Keywords

  18. NaCl对氧化淀粉浆料糊化性能的影响%Effect of NaCl on Oxidized Starch Pasting Property

    Institute of Scientific and Technical Information of China (English)

    陈蒋玲; 吴孟茹; 石点; 陈试伟; 朱谱新

    2011-01-01

    Effect of NaCl on oxidized starch pasting property was studied. Rapid viscosity analysis instrument was used to test pasting temperature, pasting time, peak viscosity, final viscosity and retrogradation value in different NaCl concentration. The result shows that oxidized starch pasting temperature, pasting time and peak viscosity is increasing gradually as NaCl to starch mass concentration increases. Final viscosity and retrogradation value fist are reduced then increased as NaCl to starch mass concentration increases. Starch sizing low temperature stability and anti-aging property is increased when NaCl to starch mass concentration is in 2% to 6% .%研究NaCl对氧化淀粉浆料糊化性能的影响.采用快速黏度仪(RVA)测试了不同NaCl浓度下氧化淀粉的糊化温度、糊化时间、峰值黏度、终值黏度和回生值.结果表明:随着NaCl对淀粉质量浓度的增大,氧化淀粉的糊化温度、糊化时间和峰值黏度逐渐增大;终值黏度和回生值随着NaC1对淀粉质量浓度的增大,先减小后增大;NaCl对淀粉质量浓度在2%~6%的范围内时,淀粉浆液的低温稳定性和抗老化性能有所提高.

  19. 海藻酸钠对海绵蛋糕品质的影响%Effect of sodium alginate on sponge cake

    Institute of Scientific and Technical Information of China (English)

    刘海燕; 王晓梅; 张娟娟; 王春霞; 范素琴; 解素花

    2014-01-01

    The effect of sodium alginate in various levels (0.1%、0.15%、0.2%、0.25% and 0.3% of flour weight)on cake quality was investigated.The results showed that:sodium alginate decreased specific grav-ity of cake batter,increased specific volume of cake,decreased the hardness and chewiness,and increased springiness and resilience of cake.It could improve the quality of cake.During longer -term storage,sodi-um alginate significantly reduced the speed to harden of cake,which indicated that sodium alginate pos-sessed good anti -aging performance,and could extend the shelf life of the cake.%主要研究了添加不同比例(0.1%、0.15%、0.2%、0.25%和0.3%)的海藻酸钠对蛋糕品质的影响。研究结果表明,海藻酸钠的引入能减小蛋糕面糊比重,增大蛋糕的比容;添加海藻酸钠后,蛋糕硬度变小,弹性和回复性增大,咀嚼性下降,对蛋糕品质有较好的改良效果。在长期放置过程中添加海藻酸钠后蛋糕的硬度增加速率显著降低,表明添加海藻酸钠有较好的抗老化效果,可延长蛋糕的货架期。

  20. Effect of Topical Probiotic on MMP-13 and Collagen III Expression in the Dermis Layer of Male Rats Irradiated with Ultraviolet-B

    Directory of Open Access Journals (Sweden)

    Vita M. Tawaran

    2016-06-01

    Full Text Available Nowadays, there is a big interest in the use of topical probiotic preparations for skin health. One of the probiotics therurapeutic benefits is used as anti-aging. During aging, there is stimulation of activator protein-1 (AP-1 which is a transcription factor that inhibits the production of collagen and AP-1 supports the breakdown of collagen by enzymes called matrix metalloproteinases (MMPs. As administration of oral Lactobacillus plantarum could inhibit skin aging by lowering the activity of MMP, so the collagen degradation can be derived so probably topical use of Lactobacillus plantarum may give more prominent effects. We used 24 male rats Sprague-Dawley strain as research objects. This study was divided into two groups, the treatment and control groups. The shaved dorsal skin of rats were irradiated with UVB three times a week for 4 weeks with total irradiation dose of 840 mJ/cm2. Skin cream, containing 247.27x107 CFU non-replicating Lactobacillus plantarum FNCC 0020, was smeared on the treatment group, two times daily, whereas the control group did not receive any treatment. Skin biopsies were done at the end of the study for examination of MMP-13 and collagen III expressions. Intensity, distribution, and histoscore of MMP-13 between the treatment and the control group showed no significant difference (p>0.05. The treatment group showed a significant different in the intensity of collagen III with the density distribution of 20–50% and the highest density was 80% (p<0.01. Administration of topical cream L. plantarum FNCC 0020 increased the expression of collagen III density distribution, but not the MMP-13 expression.

  1. Functional evaluation for effective compositions in seed oil of Korean pine%红松籽油有效成分功能评价

    Institute of Scientific and Technical Information of China (English)

    王振宇; 陈小强

    2004-01-01

    The effect of seed oil of Korean pine (Pinus koraiensis) on the rats' blood-fat and its anti-ageing function was studied for appraising the efficacy of the seed oil of Korean pine. Sixty experimental rats were randomly divided into 5 groups (half males and half females in each group) as normal control group, high fat diet control group, and three groups (Group 1 Group 2, Group 3) that were fed with feedstuff with the contents of the seed oil of 2.0g/(kg·d-1), 4.0g/(kg·d-1) and 8.0g/(kg·d-1), respectively. The indexes such as superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) and anti-oxidation capacity (AOC) were measured by Reagent Kit method. It was found that the seed oil of the Korean pine could reduce the content of triglyceride and improve SOD as well as GSH-PX activity in serum. These indexes of the rats in Group 2 fed with Korean pine seed oil of 4.0 g/(kg·d-1) reached the significant level and those of rats in Group 3 fed with the seed oil of 8.0 g/(kg·d-1) reached the extremely significant level. The results indicated the seed oil of Korean pine had function of regulating the level of blood-fat and anti-ageing.%红松是分布于中国东北的珍贵树种,其种籽油中含有丰富的生物活性物质,对人体的生理活动具有重要的调节功能.为评价红松籽油的功效,本文就红松籽油对大鼠血脂的影响及其抗衰老功能进行了研究,对不同组的大鼠喂添加了不同含量的种籽油的饲料.将60只大鼠随机地分成五组(每组雌雄各半).实验过程中,用松籽油处理的三个组的大鼠分别喂食含2.0g/(kg·d-1), 4.0g/(kg·d-1)和8.0g/(kg·d-1)剂量松籽油的饲料.用试剂盒法对超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-PX)以及抗氧化(AOC)等指标进行了测定.经研究发现:松籽油可以降低血清甘油三酯含量,提高血清SOD和 GSH-PX的活力.其中喂食4.0g/(kg·d-1)剂量红松籽油的一组,其各项测定指

  2. 冷等离子体改性CF/PMR-15复合材料耐老化性能的研究%Study on the anti-aging properties of cold plasma modified CF/PMR-15

    Institute of Scientific and Technical Information of China (English)

    张德庆; 魏月贞

    2000-01-01

    通过沸水试验、316℃高温试验、375℃热氧化试验对冷等离子体改性CF的PMR-15复合材料的抗老化性能进行研究,表明冷等离子体改性CF/PMR-15具有较好的湿热稳定性、高温性能和热氧稳定性.

  3. 新型透明质酸HyaCare Cosmetic EP的抗衰老与抗皱纹特性%Anti-aging and anti-wrinkle properties of a new hyaluronic acid HyaCare Cosmetic EP

    Institute of Scientific and Technical Information of China (English)

    唐航初

    2006-01-01

    根据近10年来医学领域的新发现,在人体皮肤中存在的透明质酸不仅扮演着保持皮肤水分、维持皮肤结构的角色,还具有促进皮肤再生、增强皮肤弹性、降解皮肤中氧自由基的功能.

  4. AW、BLE、炭黑改性沥青的老化性能研究%Research on Anti-Aging Performance of AW, BLE amd Carbon Black Modified Asphalt

    Institute of Scientific and Technical Information of China (English)

    郑远; 叶奋

    2007-01-01

    采用薄膜烘箱老化试验(TFOT)、PAV压力老化试验,对不同剂量的AW、BLE、炭黑改性沥青进行试验,利用残留针入度比、残留粘度比、软化点增量和残留延度等老化指标来分析沥青的老化程度,与常用的沥青抗老化剂SBR进行对比,研究表明0.8%AW、0.8%BLE改性沥青抗老化性能较优,炭黑改性沥青低温性能下降较大.

  5. STUDY ON ANTI-AGING FUNCTION OF NANO RED ELEMENTAL SELENIUM DISPERSED BY PROTEIN%蛋白质分散的纳米红色元素硒的延缓衰老作用

    Institute of Scientific and Technical Information of China (English)

    张劲松; 高学云; 张立德; 朱茂祥; 杨陟华

    2000-01-01

    目的考察以蛋白质为核、红色元素硒为膜和以蛋白质为分散剂的纳米红色元素硒是否具有延缓衰老作用.方法采用D-半乳糖小鼠衰老和黑腹果蝇生存模型,评价纳米红色元素硒的抗氧化和延长生存时间作用.结果纳米红色元素硒能显著降低小鼠全血丙二醛含量和提高小鼠全血谷胱甘肽过氧化物酶活性,显著延长黑腹果蝇生存时间.结论适当剂量的纳米红色元素硒具有延缓衰老保健作用.

  6. Study on Anti-aging Skin of the Polypeptides from the Sunflower Isolated Proteins%葵粕多肽抗皮肤光老化作用的研究

    Institute of Scientific and Technical Information of China (English)

    张国华; 王常青; 任海伟; 宋育璇; 伊莉

    2010-01-01

    本文以昆明小鼠为研究对象,研究了用葵粕分离蛋白加工的多肽,即葵粕多肽(SMP)对紫外线诱导的小鼠光老化皮肤的保护作用.试验动物分为五组,基础对照组不进行紫外线照射,模型组紫外线照射并涂抹蒸馏水,阳性对照组照射并涂抹Vc,大小剂量组照射并涂抹多肽样品,样品浓度分别为15%和30%.通过对小鼠皮肤组织形态的观察和皮肤生化指标分析,发现模型组小鼠皮肤表面有明显红斑,皮肤粗糙,有粗深的皱纹,葵粕多肽大剂量组小鼠皮肤皱纹已不明显,接近正常组小鼠皮肤;多肽大剂量组可降低皮肤组织中丙二醛的含量和增加羟脯氨酸的含量,差异极显著(P<0.01).研究表明:葵粕多肽有抗氧化和抗皮肤光老化的作用.

  7. 重组细胞珠蛋白抗衰老与创伤愈合的初步研究%A preliminary study of anti-aging and wound healing of recombination cytoglobin

    Institute of Scientific and Technical Information of China (English)

    李招发; 赵晓芳; 张亭亭

    2012-01-01

    本研究利用实验室纯化得到的重组细胞珠蛋白(rCygb),通过人永生化角质形成细胞(HaCAT) H2O2氧化应激模型、小鼠皮下连续注射D-gal导致的皮肤衰老模型、Cl4引起的大鼠急性肝损伤模型和大鼠皮肤创伤愈合模型,对rCygb抗氧化、增强机体抗氧化酶活性、降低氧自由基含量、延缓皮肤衰老以及创伤愈合作初步研究.结果表明:rCygb可以提高总超氧化物歧化酶(T-SOD)、谷胱甘肽过氧化物酶(GSH-Px)和过氧化氢酶(CAT)活性;降低乳酸脱氢酶(LDH)和丙氨酸氨基转移酶(ALT)活性;减少丙二醛(MDA)含量.皮肤切片展示rCygb可以促进新生血管生成、增加胶原表达和提高抗炎能力.研究结果显示:rCygb可通过改善机体清除氧自由基的能力,延缓皮肤衰老和促进创伤愈合.%In this paper, the preliminary study on antioxidant, enhancement of antioxidant enzymes activity, reducing the content of oxygen free radicals, delaying skin aging of the recombination cytoglobin (rCygb) purified by our lab were investigated through human keratinocyte cell line (HaCAT) H2O2 oxidative stress model, mouse skin aging model caused by continuous subcutaneous injection D-gal, rat acute liver injury model induced by CCI4 and rat skin wound healing model. The results showed that rCygb improved the activities of total superoxide dismutase (T-SOD), glutathione peroxidase (GSH-Px) and catalase (CAT), reduced the activities of lactate dehydrogenase (LDH) and alanine aminotransferase (ALT) as well as decreased the content of malondialdehyde (MDA). Skin biopsy showed that rCygb promoted angiogenesis, increased expression of collagen and improved the anti-inflammatory ability. All results displayed that rCygb improved the oxygen free radical scavenging ability, delayed skin aging and promoted wound healing.

  8. The effect of dietary and/or cosmetic argan oil on postmenopausal skin elasticity

    Directory of Open Access Journals (Sweden)

    Qiraouani Boucetta K

    2015-01-01

    elasticity of the skin (R5 (P<0.001, biological elasticity (R7 (P=0.001, and a significant decrease of RRT (P<0.001.Conclusion: Our findings suggest that the daily consumption and/or topical application of argan oil have an anti-aging effect on the skin demonstrated by the improvement of skin elasticity, characterized by an increase of R-parameters (R2, R5, and R7 and a decrease of RRT.Keywords: postmenopausal women, argan oil, skin elasticity

  9. Paradigm lost, paradigm found: The re-emergence of hormesis as a fundamental dose response model in the toxicological sciences

    Energy Technology Data Exchange (ETDEWEB)

    Calabrese, Edward J. [Environmental Health Sciences, School of Public Health, Morrill I, N344, University of Massachusetts, Amherst, MA 01003 (United States)]. E-mail: edwardc@schoolph.umass.edu

    2005-12-15

    This paper provides an assessment of the toxicological basis of the hormetic dose-response relationship including issues relating to its reproducibility, frequency, and generalizability across biological models, endpoints measured and chemical class/physical stressors and implications for risk assessment. The quantitative features of the hormetic dose response are described and placed within toxicological context that considers study design, temporal assessment, mechanism, and experimental model/population heterogeneity. Particular emphasis is placed on an historical evaluation of why the field of toxicology rejected hormesis in favor of dose response models such as the threshold model for assessing non-carcinogens and linear no threshold (LNT) models for assessing carcinogens. The paper argues that such decisions were principally based on complex historical factors that emerged from the intense and protracted conflict between what is now called traditional medicine and homeopathy and the overly dominating influence of regulatory agencies on the toxicological intellectual agenda. Such regulatory agency influence emphasized hazard/risk assessment goals such as the derivation of no observed adverse effect levels (NOAELs) and the lowest observed adverse effect levels (LOAELs) which were derived principally from high dose studies using few doses, a feature which restricted perceptions and distorted judgments of several generations of toxicologists concerning the nature of the dose-response continuum. Such historical and technical blind spots lead the field of toxicology to not only reject an established dose-response model (hormesis), but also the model that was more common and fundamental than those that the field accepted. - The quantitative features of the hormetic dose/response are described and placed within the context of toxicology.

  10. Is hormesis an underestimated factor in the development of herbicide resistance?

    Directory of Open Access Journals (Sweden)

    Belz, Regina G.

    2014-02-01

    Full Text Available The growing impact of herbicide resistant weeds increasingly affects weed management and the delay of resistance evolution has become a major task of chemical weed control. Hormesis and, thus, the phenomenon that low doses of herbicides can boost weed growth could be of importance in this regard since the recommended field rate may represent a low dose for weeds that have evolved resistance to the applied herbicide and, thus, a potential hormetic dose. Applying the field rate may thus not only directly select resistant biotypes, it may also indirectly promote the success and spread of resistant biotypes via hormesis. Nevertheless, hormetic effects in resistant weeds are hitherto merely randomly observed and, thus, a clear quantitative basis to judge the significance of hormesis for resistance evolution is lacking. Therefore, this study aimed at quantifying the degree and frequency of herbicide hormesis in sensitive and resistant weed species in order to provide a first indication of whether the phenomenon deserves consideration as a potential factor contributing to the development of herbicide resistance. In germination assays complete dose-response experiments were conducted with sensitive and resistant biotypes of Matricaria inodora (ALS-target-site resistant; treated with iodosulfuron-methyl-sodium/mesosulfuron-methyl, Eleusine indica (glyphosateresistant; treated with glyphosate, and Chenopodium album (triazine/triazinone-target-site resistant; treated with terbuthylazine. After 10 days of cultivation under controlled conditions plant growth was analyzed by measuring shoot/root length and mass. Results indicated that herbicide hormesis occurred on average with a total frequency of 29% in sensitive/resistant biotypes with an average growth increase of 53% occurring typically within a dose zone exceeding 350fold. Hormetic effects occurred, however, very variable and only for specific endpoints and not plant growth in general. If such a

  11. Effect of Nordic Walking training on iron metabolism in elderly women

    Directory of Open Access Journals (Sweden)

    Kortas J

    2015-11-01

    resulted in a blood Hjv increase, which was inversely correlated with the vitamin D concentration.Conclusion: Overall the Nordic Walking training applied in elderly people significantly reduced blood ferritin concentration, which explains the observed decrease in body iron stores. Keywords: ferritin, hemojuvelin, hepcidin, vitamin D, anti-agingA Letter to the Editor has been recieved and published for this article.

  12. In vitro effects of selected brominated flame retardants on the adreno cortical enzyme (CYP17). A novel endocrine mechanism of action?

    Energy Technology Data Exchange (ETDEWEB)

    Fernandez Canton, R.; Sanderson, T.; Nijmeijer, S.; Berg, M. van den [Utrecht Univ. (NL). Inst. for Risk Assessment Sciences (IRAS); Berkman, Aa. [Stockholm Univ. (Sweden). Dept. of Environmental Chemistry and Analytical Chemistry

    2004-09-15

    investigated for its relationship with anti-obesity, anti-tumor, anti-aging and anti-cancer effects. In these experiments we used the human adrenocortical carcinoma cell line (H295R cell line) to assess possible effects of these BFRs on CYP17 activity.

  13. Effects of Astragalus Polyose on Growth and Development and Consenescence of Zebra Fish%黄芪多糖对斑马鱼生长发育及衰老的影响

    Institute of Scientific and Technical Information of China (English)

    夏广清; 韩晓娟

    2011-01-01

    Taking Zebra fish as the experimental materials to study the effects of the APS on the growth and develop- ment and consenescence, the experimental results show that when the APS concentration is 0. 125mg/ml - 0.25mg/ml, the zebra fish grow and develop normally, when the concentration is too high, to 0.5mg/ ml, the growth of zebra fish was inhibited ; β- galactosidase staining ( SA - β - gal) and acridine orange (AO) staining results showed that when the concentration of APS at 0.25mg/ml, it can delay the apopto- sis of zebra fish, which play a role in anti -aging.%以斑马鱼为实验材料,研究了黄芪多糖对生长发育及衰老的影响,实验结果表明,当黄芪多糖浓度为0.125mg/ml~0.25mg/ml时,斑马鱼生长发育正常,当浓度过高,达到0.5mg/ml时,斑马鱼生长发育受到抑制;β半乳糖苷酶染色(SA—β—gal)及吖啶橙(AO)的染色结果表明,当黄芪多糖浓度为0.25mg/ml时,可以延缓斑马鱼细胞调亡,从而起到一定的抗衰老作用.

  14. A Survey of Studies on Anti-tumor Effects of LBP and Its Mechanisms%枸杞多糖的抗肿瘤作用及其机理研究现状

    Institute of Scientific and Technical Information of China (English)

    郭培培

    2011-01-01

    Lycium barbarum is a traditional Chinese medicine,which owns many medical values.LBP is the main active ingredient of Lycium barbarum,which can benefit health in anti-aging,antifatigue and so on.LBP'anti-tumor effects are introduced in the treatment of liver cancer,cervical cancer,prostate cancer,liver ascites cancer,ovarian cancer,colon cancer,cancer of the esophagus,leukemia and lung cancer,the mechanisms of which are also stated,mainly containing adjusting inmmune system and inducing the apoptosis,regulating genes and anti-oxidization.All of these are expected to be referred to for Lycium barbarum's further exploitation.%枸杞是传统常用中药,具有多种药用价值。枸杞多糖是枸杞中的主要活性成分,具有抗衰老、抗疲劳等多种保健功能,本文概述了其在肝癌、宫颈癌、前列腺癌、肝腹水癌、卵巢癌、大肠癌、食管癌、血癌、肺癌等方面的抗肿瘤作用并对其可能存在的机制如免疫系统调节机制、细胞凋亡诱导机制、基因表达调控机制、抗氧化机制做了阐述,以期为枸杞的进一步开发利用提供参考。

  15. 1-MCP对火龙果果实采后生理特性的影响%Effects of 1-Methylcyclopropene Fumigation Treatment on Physiological Characteristics of Pitaya Fruits during Storage Period

    Institute of Scientific and Technical Information of China (English)

    王彬; 郑伟; 何绪晓; 李胜海; 邓仁菊; 周俊良

    2011-01-01

    The fruits of Jinghonglong, a new pitaya variety with white flesh, were fumigated with 1 -MCP to study the effects on fruit hardness, respiration intensity, POO and CAT activity, PG and PE enzymatic activity, content of soluble pectin and insoluble pectin and discuss the anti-aging mechanism of 1-MCP in fresh preservation of pitaya during the storage period. Hie results showed that 1-MCP fumigation treatment delayed reduction of fruit hardness at the late storage period and inhibited the respiratory intensity of pitaya fruits to some extent. The 1 -MCP fumigation treatment delayed the senescence process of pitaya fruits during the earlier storage period, but it accelerated the senescence process of pitaya fruits during the late storage period.%为探明1-甲基环丙烯(1-MCP)在火龙果果实保鲜过程中的抗衰老作用机理,为火龙果保鲜提供理论依据,以白肉火龙果新品种晶红龙为试验材料,分别研究了1 -MCP处理对火龙果低温贮藏期的果实硬度、呼吸强度、POD、CAT、PG、PE、水溶性果胶和不溶性果胶的影响.结果表明,1-MCP熏蒸处理减缓了火龙果贮藏后期果实硬度下降,对火龙果果实的呼吸作用具有一定的抑制作用;贮藏前期(第24天前)可廷缓火龙果果实衰老,但是在贮藏后期却加速了果实的衰老.

  16. Cocaine synergism with alpha agonists in rat aorta: computational analysis reveals an action beyond reuptake inhibition*

    Science.gov (United States)

    Lamarre, Neil S.; Raffa, Robert B.; Tallarida, Ronald J.

    2012-01-01

    BACKGROUND Cocaine has long been known to increase blood pressure, but the degree and mechanism of vasoconstricting action remain poorly understood. Here we examine the interaction between cocaine and alpha-adrenoceptor agonists, with the action of reuptake inhibition minimized. METHODS Cocaine was administered to isolated rings of rat thoracic aorta, alone and in combination with three different adrenoceptor agonists: phenylephrine, methoxamine, and norepinephrine. Synergy analysis begins with the predicted additive effect of the combination of two agonists, based upon dose equivalence theory. This case where one agonist (cocaine) has no effect when administered alone requires only a t-test to demonstrate that a departure from additivity has occurred. RESULTS At doses where cocaine alone produced no vasoconstriction, it potentiated the vasoconstriction produced by all three alpha agonists, a clear indication of synergism between cocaine and these agents. Higher doses of cocaine in combination with alpha adrenoceptor agents gave an inverted-U shaped (hormetic) dose-effect curve, i.e., dose-related relaxation at higher doses. The hormetic dose-effect relation was analyzed using computational methodology based on dose equivalence to derive the unknown second component of action that causes relaxation. CONCLUSIONS Cocaine exhibits both vasoconstricting and vasorelaxant effects. This relaxing component, possibly related to activation of myosin light chain phosphatase, was quantified as a dose-effect curve. Most important is the synergism between cocaine and alpha-adrenoceptor stimulation which cannot be explained as an action due to reuptake inhibition, and has not been previously described. PMID:23270987

  17. Protective Effect of Hawthorn Fruit Extract on the Fat-induced Damage in Drosophila melanogaster%山楂提取物对高脂损伤果蝇的保护作用

    Institute of Scientific and Technical Information of China (English)

    赵江; 陈纯; 王红; 王轶菲; 吕晓玲; 王浩

    2016-01-01

    研究山楂提取物(Hawthorn Fruit Extract,HFE)对高脂致氧化损伤的雄性果蝇寿命的影响并探究其延缓果蝇衰老的分子机制。将2 d龄雄性果蝇随机分组,在高脂培养基中饲喂不同剂量(0.5、1.5、4.5 mg/mL)山楂提取物,研究山楂提取物对果蝇生存寿命及果蝇体内抗氧化酶活性(铜锌超氧化物歧化酶,Copper-zinc-containing superoxide dismutase,Cu/Zn-SOD;锰超氧化物歧化酶Manganese-containing superoxide dismutase,Mn-SOD;过氧化氢酶,Catalase,CAT)、脂质过氧化产物丙二醛(Malonaldehyde,MDA)含量、蛋白质羰基(Protein Carbonyl ,PCO)含量及抗氧化相关基因表达水平的影响。HFE可以显著延长果蝇的平均寿命和最高寿命(4.5 mg/mL,P<0.05);4.5 mg/mL HFE可以显著提高果蝇体内SOD和CAT活性(P<0.05),显著降低MDA(P<0.01)和PCO含量(P<0.05);同时显著上调果蝇体内Cu/Zn-SOD及Nrf2 mRNA表达水平(P<0.05)并且极显著上调Mn-SOD和CAT mRNA表达水平(P<0.01)。HFE对高脂膳食所导致的果蝇氧化损伤具有保护作用,HFE延缓果蝇衰老和抗氧化作用可能与其调控抗氧化基因的表达水平相关。%To explore the effect of hawthorn fruit extract on lifespan in male Drosophila melanogaster damaged by high fat and the underlying mechanism of anti-aging. Male Drosophila melanogaster within 2 d-old were treated with different doses (0.5,1.5,4.5 mg/mL) of HFE. The lifespan,anti-oxidation enzymes activities and their encoding genes expressions were evaluated. Dietary addition of HFE could prolong the lifespan of Drosophila melanogaster significantly(especially at 4.5 mg/mL). Given HFE 4.5 mg/mL made the enzyme activ-ity of SOD and CAT increase markedly(P<0.05)with decreasing the content of malondialdehyde(MDA)(P<0.01)and protein carbonyl (PCO)(P<0.05);moreover,a significant increase in Cu/Zn-SOD and CAT mRNA expression (P<0.05) as well as Mn-SOD and

  18. Effect of palygorskite coated slow-release fertilizer on economic characters and physiological indexes of Brussica napus%坡缕石包衣缓释肥对油菜经济性状及生理指标的影响

    Institute of Scientific and Technical Information of China (English)

    尚千涵; 蔺海明; 邱黛玉; 刘伟生; 罗春燕; 曹发魁; 宋颖

    2009-01-01

    通过盆栽试验,研究了坡缕石包衣肥的增产及缓释效应.结果表明:施用坡缕石包衣的磷酸二铵225 kg·hm~(-2)后,油菜有效结角数和经济产量分别较对照提高了2.27 %和255.33 %;油菜后复种小麦,施用坡缕石包衣肥300 kg·hm~(-2)后,小麦生物产量比对照增加了57.67 %.在油菜生育后期,施用坡缕石包衣肥后油菜叶绿素、蛋白质、可溶性糖含量均较对照高,丙二醛含量较对照低,说明施用坡缕石包衣肥可提高生育后期油菜的抗衰老性和抗逆性,缓释效果较好.%The yield-increasing and slowly releasing effects of palygorskite coated fertilizer were investigated with pot experiment. The result showed that application of diammonium phosphate coated with paly-gorskite at 225 kg · hm~)(-2),the effective siliquae and yield of rape (Brussica napus) increased by 2. 27 % and 255. 33 % respectively compared with control. The biomass of wheat which planted after rape increased by 57. 67 % when compared with control at 300 kg ·hm~(-2). The content of chlorophyll, protein and soluble sugar in rape at latter growth stage were higher than that of the control, while MDA content was lower. It is suggested that palygorskite coated fertilizer shows good anti-aging and adverse-resistance characteristics, it also has certain slow release property on diammonium phosphate.

  19. Progress of Textual Research and Pharmacological Effects on Erzhi Pills%二至丸考源及药理作用研究进展

    Institute of Scientific and Technical Information of China (English)

    蔡秀江; 黄美艳; 丁安伟; 姚卫峰; 张丽

    2011-01-01

    The origin of Erzhi pills and pharmacological effects were reviewed and analyzied. The ancient medical literatures and CNKI database were studied, the origin of Erzhi pills and pharmacological effects of the new progress were summarized and classified. It was indicated that it might derive from Fushou Jingfang of the Ming dynasty. Modern pharmacological studies have shown that it can be used in liver protection, liver fibrosis, anti-aging, immune function regulation, shortening the coagulation time, improving blood rheology, inhibiting rumor, increasing intelligence, anti-inflammatory, decreasing blood sugar, resisting fatigue. In recent years, study on the liver protection has more reports, other pharmacological effects, for instant the kidney Yin, need to be strengthen. Related animal model need to be established. It is suggest that further mechanism research, pharmacokinetic and metabolomics research should be proceeded. Material basis of therapeutic effects should be cleared in order to provide a basis of clinical application and further development.%对二至丸的来源和药理作用研究进行文献整理和分析.通过查阅古代医药文献及中国知网数据库,对二至丸方剂的来源和在药理作用研究方面取得的新进展,进行归纳整理并分类汇总.考证二至丸出自明代《扶寿精方》;现代药理作用表明,二至丸具有保肝降酶、抗肝纤维化、抗衰老、调节免疫机能、缩短血液凝血时间、改善血液流变性、抑制肿瘤、益智、抗炎、降血糖、抗疲劳等作用.近年来对其保肝作用研究较多,还需加强补肾滋阴等其他药理作用的研究,建立相关动物模型.建议对二至丸进行深入的作用机制研究,加强药代动力学及代谢组学研究,明确其发挥药效作用的物质基础,以期为二至丸临床应用和进一步开发提供依据.

  20. Effect of Lycium barbarum Polysaccharides on the expression of endothelin-1 and its receptors in an ocular hypertension model of rat glaucoma

    Institute of Scientific and Technical Information of China (English)

    Xue-Song Mi; Kin Chiu; Geoffrey Van; Justin Wai Chung Leung; Amy Cheuk Yin; Sookja Kim Chung; Raymond Chuen-Chung Chang; Kwok-Fai So

    2012-01-01

    Lycium barbarum, a traditional Chinese anti-aging herb, has been shown to protect retinal ganglion cells (RGCs) in a rat chronic ocular hypertension (COH) model.Here, we investigated the expression of endothelin-1 (ET-1), a strong vasoconstrictor, and its receptors, ETA and ETB, in the COH model and assessed the effects of Lycium barbarum on the ET-1 axis.Elevated intraocular pressure (IOP) was induced in the right eye of SD rats using argon laser photocoagulation.(1) The expression of ET-1, ETA and ETB in normal and COH retinas was studied.(2) Some COH rats were fed daily with Lycium barbarum Polysaccharides (LBP) using 1 mg/kg or phosphate-buffered saline (PBS) for 3 weeks (started 1 week before photocoagulation).The effects of LBP on the expression of ET-1 and its receptors, ETA and ETB, in COH retina were evaluated.A semi-quantitative analysis of staining intensity was used to evaluate the expression levels of ET-1, ETA and ETB in retinal vasculature.We found that (1) Under COH condition, the immunoreactivity of ET-1 was increased in retina associated with an increase of ETB receptor immunoreactivity and a decrease of ETA receptor immunoreactivity.(2) After feeding COH rats with LBP, the expression of ET-1 was decreased with an increase of ETA expression and a decrease of ETB expression in the retina, especially in RGCs.(3) By comparing the staining intensity in the vasculature of COH retina in LBP-fed group with PBS-fed group, there was a decrease in the expression of ET-1 and ETA and an increase in ETB.In summary, ET-1 expression was up-regulated in the retina in COH model.LBP could decrease the expression of ET-1 and modulate the expression of its receptors, ETA and ETB, under the condition of COH.The neuroprotective effect of LBP on RGCs might be related to its ability to regulate the ET-1-mediated biological effects on RGCs and retinal vasculature.

  1. The effectiveness of a standardized rose hip powder, containing seeds and shells of Rosa canina, on cell longevity, skin wrinkles, moisture, and elasticity

    Directory of Open Access Journals (Sweden)

    Phetcharat L

    2015-11-01

    Full Text Available L Phetcharat,1,2 K Wongsuphasawat,1,2 K Winther31School of Antiaging and Regenerative Medicine, Mae Fah University, Bangkok, Thailand; 2Department of Anti-aging and Regenerative Medicine, Mae Fah University, Bangkok, Thailand; 3Institute for Nutrition, Exercise and Sports, University of Copenhagen, Frederiksberg C, DenmarkObjective: To evaluate the effects of a rose hip powder (Hyben Vital® made from seeds and shells on cell senescence, skin wrinkling, and aging.Methods: A total of 34 healthy subjects, aged 35–65 years, with wrinkles on the face (crow’s-feet were subjected to a randomized and double-blinded clinical study of the effects of the rose hip powder, as compared to astaxanthin, a well-known remedy against wrinkles. During the 8-week study, half of the participants ingested the standardized rose hip product, while the other half ingested astaxanthin. Objective measurements of facial wrinkles, skin moisture, and elasticity were made by using Visioscan, Corneometer, and Cutometer at the beginning of the study, after 4 weeks, and after 8 weeks. Evaluation of participant satisfaction of both supplements was assessed using questionnaires. In addition, the effect of the rose hip preparation on cell longevity was measured in terms of leakage of hemoglobin through red cell membranes (hemolytic index in blood samples kept in a blood bank for 5 weeks. Significance of all values was attained with P≤0.05.Results: In the double-blinded study, the rose hip group showed statistically significant improvements in crow’s-feet wrinkles (P<0.05, skin moisture (P<0.05, and elasticity (P<0.05 after 8 weeks of treatment. A similar improvement was observed for astaxanthin, with P-values 0.05, 0.001, and 0.05. Likewise, both groups expressed equal satisfaction with the results obtained in their self-assessment. The rose hip powder further resulted in increased cell longevity of erythrocyte cells during storage for 5 weeks in a blood bank

  2. 人参皂苷 Rg1对心血管系统和神经系统药理作用的研究进展%Advances in pharmacological effects ofGensenoside Rg1 on The cardiovascular system and The nervous system

    Institute of Scientific and Technical Information of China (English)

    董艳红; 胡婷婷; 代良萍; 谢晓芳; 彭成

    2016-01-01

    Gensenoside Rg1 is one of the main active principles in ginseng.Modern pharmacological studies show that Gensenoside Rgh1 as many biological activities on many systems of the body,especially for the influence of the cardiovascular system and nervous system widely.Thisarticle through to retrieve nearly 10 years pharmacological research literature of gensenoside Rg1 ,in the two aspects, Results show that gensenoside Rg1 with protecting the myocardial,anti -myocardial ischemia and promoting the regeneration of blood vessel and anti -myocardial hypoxia injury in the cardiovascular system;Against cerebral ischemia/reperfusion injury in the nervous system,nootropic,antioxidant and anti -aging effects in the nervous system.%人参皂苷 Rg1是人参中的重要成分之一。现代药理学研究显示人参皂苷 Rg1在对人体的很多方面具有很强的药理活性,特别是在心血管系统和神经系统。本文通过检索近10年来人参皂苷 Rg1心血管系统和神经系统药理研究文献,综述人参皂苷 Rg1在此两个方面的药理作用,结果表明人参皂苷 Rg1在心血管系统有保护心肌细胞、抗心肌缺血和促血管再生作用;对神经系统有抗脑缺血再灌注损伤、益智、抗神经细胞氧化损伤的作用,为其药物开发提供依据。

  3. Redox-dependent induction of antioxidant defenses by phenolic diterpenes confers stress tolerance in normal human skin fibroblasts: Insights on replicative senescence.

    Science.gov (United States)

    Carvalho, Ana C; Gomes, Andreia C; Pereira-Wilson, Cristina; Lima, Cristovao F

    2015-06-01

    Mild stress-induced hormesis represents a promising strategy for targeting the age-related accumulation of molecular damage and, therefore, for preventing diseases and achieving healthy aging. Fruits, vegetables, and spices contain a wide variety of hormetic phytochemicals, which may explain the beneficial health effects associated with the consumption of these dietary components. In the present study, the induction of cellular antioxidant defenses by the phenolic diterpenes carnosic acid (CA) and carnosol (CS) were studied in normal human skin fibroblasts, and insights into the aging process at the cellular level investigated. We observed that CA and CS induced several cytoprotective enzymes and antioxidant defenses in human fibroblasts, whose induction was dependent on the cellular redox state for CS and associated with Nrf2 signaling for both compounds. The stress response elicited by preincubation with CS conferred a cytoprotective action against a following oxidant challenge with tert-butyl hydroperoxide, confirming its hormetic effect. Preincubation of normal fibroblasts with CS also protected against hydrogen peroxide-induced premature senescence. Furthermore, cultivation of middle passage normal human skin fibroblasts in the presence of CS ameliorated the physiological state of cells during replicative senescence. Our results support the view that mild stress-induced antioxidant defenses by CS can confer stress tolerance in normal cells and may have important implications in the promotion of healthy aging.

  4. Effects of Ultra-drying on Vigor and Physiological characteristics of Hippophae rhamnoides Seeds

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    Studies of ultra-drying and accelerated aging on Hippophae rhamnoides seeds from the arid and semiarid areas of northwest China were conducted. The results showed that H. rhamnoides seeds had higher desiccation endurance. When seeds were desiccated to 26 g/kg, their germination percentage, vigor indices and enzyme activity could be retained. After 30-day accelerated aging, ultra-dried seeds had better anti-aging ability and storability compared to natural dried seeds, and the best choice for the MC of seed ...

  5. 烷基硫代磷酸锌硫化促进剂对天然橡胶老化性能影响研究%Effect of Dialhydithiophate Vulcanizing Accelerator on Aging of Vulcanized Natural Rubber in Hot Air

    Institute of Scientific and Technical Information of China (English)

    赵华松; 李建雄; 刘小波; 刘安华

    2011-01-01

    Natural rubber was vulcanized with the aid of zinc dialkydithiophosphates and other common vulcanizing accelerators and the resultant samples were aged in hot air. The mechanical properties of the samples were measured before and after aging and the anti-aging performance were compared. The experiment results indicated that the sample with zinc diisopropyldithiophate possessed excellent anti-aging performance.%研究了烷基硫代磷酸锌和其他常用硫化促进剂对天然橡胶硫化胶的老化性能的影响.实验表明二异丙基二硫代磷酸锌能赋予天然橡胶硫化胶优良的耐热空气老化性能.

  6. Efficacy of Microneedling Plus Human Stem Cell Conditioned Medium for Skin Rejuvenation: A Randomized, Controlled, Blinded Split-Face Study

    OpenAIRE

    Lee, Hee Jung; Lee, Eo Gin; Kang, Sangjin; Sung, Jong-Hyuk; Chung, Hyung-Min; Kim, Dong Hyun

    2014-01-01

    Background The use of growth factors in skin rejuvenation is emerging as a novel anti-aging treatment. While the role of growth factors in wound healing is well established, their use in skin rejuvenation has only recently been to be studied and no controlled trials have been performed. Objective We evaluated the anti-aging effects of secretory factors of endothelial precursor cells differentiated from human embryonic stem cells (hESC-EPC) in Asian skin. Methods A total of 25 women were inclu...

  7. Exercise Attenuates the Major Hallmarks of Aging

    Science.gov (United States)

    Garatachea, Nuria; Pareja-Galeano, Helios; Santos-Lozano, Alejandro; Fiuza-Luces, Carmen; Morán, María; Emanuele, Enzo; Joyner, Michael J.; Lucia, Alejandro

    2015-01-01

    Abstract Regular exercise has multi-system anti-aging effects. Here we summarize how exercise impacts the major hallmarks of aging. We propose that, besides searching for novel pharmaceutical targets of the aging process, more research efforts should be devoted to gaining insights into the molecular mediators of the benefits of exercise and to implement effective exercise interventions for elderly people. PMID:25431878

  8. Placebo Effect

    Science.gov (United States)

    ... C. Spencer, MD Steven Karceski, MD The placebo effect Joseph H. Friedman, MD Richard Dubinsky, MD WHAT ... placebo: a “dummy” medication that should have no effect on the condition. Placebos are not only drugs. ...

  9. Effect and mechanism of intense pulsed laser on skin aging in rats%强脉冲激光对小鼠皮肤老化的作用及其机制

    Institute of Scientific and Technical Information of China (English)

    向亚平; 王斌; 鲁健云; 陈静; 左成忻; 谭丽娜; 黄进华

    2009-01-01

    目的:探讨强脉冲激光(IPL)治疗皮肤老化的作用机制.方法:用IPL在一定能量密度照射小鼠老化皮肤, 连续照射2次,间隔为2周,观察小鼠皮肤中SOD 活力,MDA和Hyp含量及凋亡相关基因蛋白Bax,Bcl-2表达水平的变化并与模型组和正常对照组比较.结果: IPL可使小鼠皮肤中SOD 活力、Hyp含量增加,MDA 含量降低,较模型组改变明显(P<0.05).IPL照射后小鼠皮肤Bcl-2蛋白表达增高,而对Bax 蛋白影响不明显.结论:IPL对改善皮肤老化有效,其作用机制可能与改善皮肤氧化和抗氧化之间的平衡,恢复氧化酶的活性及提高凋亡抑制基因蛋白Bcl-2的表达,调节皮肤凋亡有关.%Objective To explore the effect of intense pulsed laser (IPL) on anti-aging and its mechanism.Methods Rat skin was continuously irradiated ex vivo by IPL at certain wavelengths with different energy densities. The rats were irradiated twice with an interval of 2 weeks. We measured superoxide dismutase (SOD) activity, malondialdehyde (MDA) content, hydroproline (Hyp) content, and the level of apoptosis-related gene B-cell lymphoma-2 (Bcl-2) and Bcl-2 associated X protein (Bax) in the IPL treatment groups, and then compared with those in the model groups and the control group.Results SOD activity and Hyp content in the IPL treatment groups were higher than those in the model groups, and MDA content in the IPL treatment group was lower than that in the model group (P<0.05). IPL irradiation increased Bcl-2 protein content in rat skin cells, but had no effect on Bax protein expression.Conclusion IPL is effective to improve the appearance of aging skin. Its mechanism may relate to maintaining the balance of oxidation and anti-oxidation, restoring oxidase activity, and regulating the death of skin cells by increasing Bcl-2 expression.

  10. Systems effectiveness

    CERN Document Server

    Habayeb, A R

    1987-01-01

    Highlights three principal applications of system effectiveness: hardware system evaluation, organizational development and evaluation, and conflict analysis. The text emphasizes the commonality of the system effectiveness discipline. The first part of the work presents a framework for system effectiveness, partitioning and hierarchy of hardware systems. The second part covers the structure, hierarchy, states, functions and activities of organizations. Contains an extended Appendix on mathematical concepts and also several project suggestions.

  11. Studies on the anti-radiation activity of ashwagandha

    Energy Technology Data Exchange (ETDEWEB)

    Gandhi, R.; Kaul, B.L. (Regional Research Lab., Jammu (India))

    1984-12-14

    Experiments were carried out to see if root extracts of Withania somnifera (ashwagandha), a reputed anti-ageing drug of 'Ayurveda', gives protection against the simulated ageing effects of ionizing radiations. Strong radioprotective ability was shown by the drug when it was found to be associated with the presence of steroidal lactones in the extract.

  12. "Further Effects"

    Science.gov (United States)

    Kinigstein, Steven Michael

    In writing Further Effects, I intended to illustrate the benefits that are to be had from the use of effects - processing, when applied at the compositional level, rather than as a post-compositional afterthought. When effects are used creatively in the compositional stage, they will influence the very nature of a piece. They are capable of expressing rhythmic and metric ideas. They can alter the natural timbre of an instrument. This can be done on levels of abstraction ranging from discreet subtlety to disguise beyond recognition. There is one effect (known as "pitch shift.") that allows an instrument to play pitches that are well outside of its range. In Further Effects, I direct the performers to use a volume pedal (which I view as a tool, rather than an effect) for the broadened creative use of dynamics that it so efficiently grants. The use of an effects processor and volume pedal creates a need for ancillary equipment. An amplifier, cables, and an electric hook-up (a microphone or a pickup) will be required for each instrument. While an amplifier serves to project the processed sound, there must also be a device or method to suppress unprocessed sound. A great deal of thought and work goes into the use of effects; yet I feel it is wasteful to use this musical resource merely as post-compositional decoration.

  13. Effect of isolated soybean protein on the lifespan of drosophila melanogasters%大豆分离蛋白对果蝇寿命的影响

    Institute of Scientific and Technical Information of China (English)

    韩加; 付德润; 徐臻荣; 郭伟; 吐尔逊江; 王运诚

    2005-01-01

    BACKGROUND: Many data demonstrate that the components of soybean can lower blood lipid,suppress the growth of cancer cells and exert weak estrogenic activities. However,little is known about the effect of isolated soybean protein on the lifespan of the organism.OBJECTIVE: To observe the effect of isolated soybean protein on the lifespan of drosophila melanogasters and investigate the mechanism of effective anti-aging and anti-oxidation action.DESIGN: A controlled trial based on drosophila melanogasters.SETTING: Department of nutrition and food hygiene in a university.MATERIALS: The experiment was conducted in the Department of Nutrition and Food Hygiene,Public Health College of Xinjiang Medical University from March to June 2002. A total of 400 drosophila melanogasters of American wild type with half for each gender were provided by the Department of Nutrition and Food Hygiene, Public Health College of Xinjiang Medical University.METHODS: The 400 drosophila melanogasters were divided into control group(normal culture) and three-dosage experiment groups(normal culture contained isolated soybean protein 0.2%,1.0% and 5.0%,respectively).From the second day on, the number of living and dead drosophila melanogasters was observed and counted until all died. Meanwhile, mean lifespan,half death time and maximal lifespan were calculated.MAIN OUTCOME MEASURFS: Mean lifespan, half death time and maximal lifespan of the drosophila melanogasters.RESULTS: Compared with that of control group, the lifespan of male and female drosophila melanogasters in experiment groups was prolonged by isolated soybean protein and responded in a dose-dependent manner,especially in high-dosage group. The mean lifespan, half death time and maximal lifespan of both female and male drosophila melanogasters were prolonged by 24.5% and20.7%,27.1% and22.0%,and 13.9% and 10.6%,respectively.CONCLUSION: Isolated soybean protein may have anti-aging and lifespan-prolonging effects on drosophila melanogasters

  14. 白藜芦醇对快速老化鼠肝脏抗氧化能力的影响%Effect of resveratrol on antioxidant abilities of liver in senescence accelerated mice

    Institute of Scientific and Technical Information of China (English)

    刘贵珊; 杨博; 范艳丽; 张泽生; 贺伟

    2012-01-01

    To observe the effect of resveratrol on antioxidant abilities of liver in senescence accelerated mice. Four-month old SAMP/8 mice were divided into four groups randomly: The model group, resveratrol low- dose, moderate-dose and high-dose groups, and four-month old SAMR/1 mice were in normal control group. The normal control group and model group were given normal saline by garage, Res-treated mice were administered Res by garage at the daily doses of 25 mg/kg, 50 mg/kg and 100 mg/kg respectively. After medication for 8 weeks,superoxide dismutase (SOD), malondialdehyde (MDA) in the liver of mice were determined, histological changes in mice liver tissue were observed after HE stain. Resveratrol could significantly increase activities of SOD (P〈 0.01 ), decrease the content of MDA (P% 0.01 ) in liver tissue and ameliorate histological structure of liver tissue. Resveratrol could significantly improve antioxidant abilities of liver in SAMP/8 mice and have anti-aging effect.%分析白藜芦醇对快速老化鼠(SAMP/8)肝脏抗氧化能力的影响,将4月龄雄性SAMP/8鼠随机分为模型组和白藜芦醇低、中、高剂量纽,4月龄正常老化sAMR/1鼠为正常对照组.对照组及模型组用生理盐水灌胃,白藜芦醇治疗组给予25、50和100mg/kg白藜芦醇灌胃.连续给药8周,测定各组小鼠肝组织超氧化物歧化酶(SOD)、丙二醛(MDA)的含量,以HE染色进行肝组织形态学观察.结果表明:白藜芦醇各剂量组能提高sAMP/8鼠肝组织SOD活性(P〈0.01),降低MDA含量(P〈0.01),且能改善肝组织形态结构,尤以高剂量组效果显著.因此,白藜芦醇能显著提高SAMP/8鼠肝组织的抗氧化能力,具有一定的延缓衰老作用.

  15. 红景天抗氧化及对细胞因子作用的研究进展%Research progress in the effects on antioxidantion and cytokines of rhodiola

    Institute of Scientific and Technical Information of China (English)

    贺佳林

    2015-01-01

    Increasing researches reported that rhodiola has the effects of anti-oxidation, anti-inflammation, and anti-aging. Salidroside could eliminate free radicals such as(□)O2,(□)OH and lipid oxygen free radical, inhibit lipid peroxidation reaction and improve the activity of key enzymes including SOD, GSH-Px and CAT, which promotes the clearance of endogenous free radicals. These data demonstrated that the mechanism underlying the anti-oxidantion of rhodiola involves the inhibition of lipid peroxidation and the elimination of free radicals. Furthermore, salidroside could inhibit IL-1β, IL-6, IL-18, TNF-α and TGF-β1 expression, and increase IFN-γ level, indicating that the regulation of cytokines expression mediates the anti-inflammatory effect of rhodiola. This paper summarized the anti-oxidantion and cytokines of Rhodiola based on previous researches.%大量研究表明红景天具较好的抗氧化、抗炎及抗衰老作用.红景天苷能清除(□)O2、(□)OH、脂氧自由基等,抑制脂质过氧化,并且提高内源性自由基清除系统中关键酶SOD、GSH-Px和CAT等的活性,表明红景天苷的抗氧化作用主是通过调控自由基的清除和抗氧化酶的活性而实现的.红景天苷还能抑制炎性细胞因子IL-1β、IL-6、IL-18以及TNF-α、TGF-β1的表达和显著增加IFN-γ的水平,表明其抗炎作用主是通过调节细胞因子的表达而实现的.本文就近年来有关红景天所具有的抗氧化及细胞因子作用研究现状进行了综述.

  16. Effects of Ginsenoside Rg1 on the Expression of Toll-Like Receptor 3, 4 and Their Signalling Transduction Factors in the NG108-15 Murine Neuroglial Cell Line

    Directory of Open Access Journals (Sweden)

    Bao-Sheng Zhao

    2014-10-01

    Full Text Available As one of the most important components of Panax ginseng, ginsenoside Rg1 has certain anti-aging effects, improving the activity of learning and memory. Studies have showed that ginsenoside Rg1 improves the memory impairment associated with Alzheimer’s disease (AD. In this study, the effects of ginsenoside Rg1 were investigated through the activity of toll-like receptor (TLR 3, TLR4 and their signaling transduction pathways in amyloid β peptide 25–35 (Aβ25–35 induced AD cell model. Thus we investigated several critical components of the TLR pathway. The neuroglial cell line NG108-15 was stimulated with or without Aβ25–35, while different concentrations of ginsenoside Rg1 were administered. After 24 h, tumor necrosis factor-α (TNF-α, interferon-β (IFN-β in cell supernatant and inducible nitric oxide synthase (iNOS in cell lysate supernatant were measured with enzyme-linked immunosorbent assays (ELISAs. The mRNA and protein expression of TLR3, TLR4, nuclear factor kappa B (NF-κB and tumor necrosis factor receptor-associated factor-6 (TRAF-6 were detected by real-time PCR and western blot methods, respectively. The experimental results showed that Aβ25–35 could markedly raise the level of TNF-α, IFN-β and iNOS, and increase the expressions of mRNA and TLR3, TLR4, NF-κB and TRAF-6 protein in the NG108-15 cells. At the same time, the ginsenoside Rg1 significantly reduced the expressions of proteins and mRNA of TLR3, TLR4, NF-κB and TRAF-6, and down-regulated the levels of TNF-α, IFN-β of cell supernatant and iNOS of cell lysate supernatant in a concentration-dependent manner. In conclusion, ginsenoside Rg1 has good activity for suppressing the signaling transduction pathway of TLR3 and TLR4, and decreasing the inflammation factors induced by Aβ25–35 in NG108-15 cells, and this may be the mechanism of ginsenoside Rg1 action in AD treatment, but more studies are needed to identify its specificity.

  17. Observation of the effect of Hongyangfuzheng oral liquid on exercise capacity and aging mechanism%红阳扶正口服液增强运动能力和抗衰老机理的实验观察

    Institute of Scientific and Technical Information of China (English)

    董彩霞; 张彩云; 滕宝霞

    2012-01-01

    目的 研究红阳扶正口服液对增强运动能力和抗衰老作用的机理.方法 小鼠口服红阳扶正口服液10 d,每鼠尾部负重为体重的5%,力竭后,摘取眼球取血,3000 r/min离心10 min,检测血清乳酸、乳酸脱氢酶、肝糖原、尿素氮、丙二醛和超氧化物歧化酶(SOD),测定红阳扶正口服液对衰老模型小鼠脑中SOD和丙二醛含量的影响.结果 红阳扶正口服液能明显降低血清中乳酸、尿素氮、丙二醛的含量,增加小鼠血清中乳酸脱氢酶、SOD和肝糖原的含量(P<0.05);红阳扶正口服液降低衰老模型小鼠大脑中丙二醛的含量,增加大脑SOD活性.结论 红阳扶正口服液具有增加运动能力和抗衰老的作用.%Objective To investigate the effect of Hongyangfuzheng oral liquid (HOL) on exercise capacity and aging mechanism. Methods Through oral administration with HOL 10 days, each rat tail weight for weight 5%, after exhaustive, removal of eye blood, centrifugal 10 min (3000 r/min). The content of serum lactic acid (LA), lactate dehydrogenase (LAD), hepatic glycogen, blood urea nitrogen (BUN), malondialdehyde (MDA) and superoxide dismutase (SOD) was detected. MDA and SOD in the brain of mice was detected too. Results HOL obviously decrease the content of serum LA, BUN, MDA. LAD, SOD and hepatic glycogen increased in mice serum (P<0.05). HOL reduced the aging model mice brain MDA content, increased SOD activity in brain. Conclusion HOL can increase of exercise capacity and the anti aging effect.

  18. Effect of Tea tree oil, Eugenol and Citral on keeping quality of litchi%茶树油、丁香酚、柠檬醛在荔枝保鲜中的应用

    Institute of Scientific and Technical Information of China (English)

    钟业俊; 徐欣源; 刘成梅; 刘伟

    2011-01-01

    本文研究了茶树油、丁香酚和柠檬醛的挥发性香氛对荔枝的保鲜作用。采用复配方法探究三种香料对荔枝的协同保鲜效果。结果表明:在冷藏(4±1℃)过程中,茶树油和丁香酚、柠檬醛的挥发性香氛可抑制荔枝果实腐败,延缓果皮衰老和果肉维生素C含量的降低,对保持荔枝果实的感官品质也具有积极作用;且三者复配后效果更佳,贮藏至18天,复配组荔枝的腐败率、果皮相对渗透率、维生素含量分别为对照组的56.0%、72.8%、124.8%,果皮颜色、果实滋味、果实气味和整体评价分别比对照组高2.4、3.3、2.6和3.4分。%The preservation effect of volatile fragrance of tea tree oil, eugenol and citral "to litchi decay and the synergistic inhibition (of these three combined) were studied. The results showed that with the refrigeration storage condition (4±1℃), volatile fragrance of tea tree oil, eugenot and citral had a inhibition and retard effect on the rise in the rot rate of litchi and the decrease of Vitamin C content in pulp, meanwhile, they also showed a positive anti-aging and preservation quality for keeping fresh of litchi. All of these positive abilities enhanced after these three spices mixed. After 18 days storage, the rot rate, pericarp relative leakage and Vitamin C content in litchi treated with composite solution are 56.0%, 72.8%, 124.8% of the values of the control group, and the evaluation scores on skin color, fruit taste, smell and overall were 2.4, 3.3, 2.6 and 3.4 points respectively higher than the control group.

  19. The Protective Effect of Haworth Fruit Extract on Caenorhabditis elegans in Acute Damages%山楂提取物对急性损伤线虫的保护作用

    Institute of Scientific and Technical Information of China (English)

    赵江; 陈纯; 王轶菲; 王红; 黄杰; 王浩

    2016-01-01

    以秀丽隐杆线虫(Canorhabditis elegans)为模式生物研究山楂提取物(Haworth fruit extract,HFE)对其急性氧化损伤的保护作用及其可能的作用机制。饲喂线虫于含有不同浓度(0、25、50和100μg/mL)HFE的NGM(Nematode growth medium)培养基中,研究HFE对线虫急性应激耐受能力的影响。结果显示,饲喂HFE后,秀丽线虫表现出比正常组更高的寿命,并且在胡桃醌氧化应激、热应激及紫外辐射应激实验中寿命均明显延长,荧光显微镜观察发现HFE组线虫的脂褐素自发荧光明显减弱,并且与HFE浓度呈剂量依赖效应。HFE能够显著延长秀丽隐杆线虫的寿命,同时对多种氧化损伤具有较好的保护作用,改善机体的抗氧化能力,有效延缓衰老。%The work aims to study the protective effect of haworth fruit extract(HFE)on the acute oxidative damage and its possible protection mechanism in Caenorhabditis elegans. The C. elegans were fed with nematode growth medium(NGM)containing different dose of HFE(0,25,50 and 100μg/mL)to study impacts of HFE on the acute stress tolerance. C. elegans with feeding in different dose of HFE had a longer lifespan than the normal group,moreover,their lifespans became significantly longer under the experiments of juglone oxidative stress, heat stress and UV radiation stress. Concurrently,the autofluorescence level of lipofuscin in HFE group of C. elegans significantly decreased and it was dose-dependent effect to the concentration of HFE. In conclusion,HFE significantly prolongs the lifespan of C. elegans and has a solid protection under a variety of oxidative stress conditions with improving the antioxidant capacity and efficient anti-aging.

  20. Health Effects

    Science.gov (United States)

    ... CDC Policy Partners Climate Effects on Health Air Pollution Allergens Wildfires Temperature Extremes Precipitation Extremes Diseases Carried by Vectors Food and Waterborne Diarrheal Disease Food Security Mental Health and Stress-Related Disorders Climate-Ready States and ...

  1. Facebook Effect

    OpenAIRE

    STOICA, Anamaria

    2011-01-01

    This research paper is intended to understand the effects that Facebook, the social networking site has upon us, whether it influences our lives in a good or in a bad way. In order to understand the Facebook Effect we are trying to see how it impacts our lives at economic level,social level, political level, terminology level , psychological level and cultural level . Starting from the question : What does Facebook want? we found several answers consisting in pros and cons of this phenomenon ...

  2. Effective Programming

    DEFF Research Database (Denmark)

    Frost, Jacob

    To investigate the use of VTLoE as a basis for formal derivation of functional programs with effects. As a part of the process, a number of issues central to effective formal programming are considered. In particular it is considered how to develop a proof system suitable for pratical reasoning......, how to implement this system in the generic proof assistant Isabelle and finally how to apply the logic and the implementation to programming....

  3. Effects of

    OpenAIRE

    Gowthaman, S.; A. P. Sathiyagnanam

    2016-01-01

    This work investigates the effect of both inlet air temperature and fuel injection pressure on performance and emission behaviour of homogeneous charge compression ignition engine (HCCI) fuelled with diesel fuel. In this investigation, HCCI engine operates with different inlet air temperature and fuel injection pressure, and analysis the effect of these variables on HCCI engine performance and emissions. The inlet air temperatures are varied between 40 °C and 70 °C and the injection pressure ...

  4. Effects of the compound gum on the rheological properties of paste and its application in bread%复配胶对面团流变性的影响及其在面包中的应用

    Institute of Scientific and Technical Information of China (English)

    李崇高; 何鑫磊; 黄建初; 庞杰; 何桂丽; 郑玉玺

    2011-01-01

    Glucomannan, carrageenan and soy protein isolate were compound to study the dough silty, tensile properties and bread physicochemical and sensory index. The results showed; increase of konjac glucomannan. Carrageenan reduced dosage, can increase the dough water absorption, development time, increase the number of draw ratio, while tensile energy, extensibility decreases) dough evaluation of the value of the first increased and then decreased. Add soy protein isolate certain amount of time, will increase the water absorption, longer development time. Glucomannan, carrageenan and soy protein isolate added to the optimal ratio of 2 ' 1 ' 25 flour, wheat flour mixed gum when added amounted to 3%, enhance network structure modulation dough, bread, water , specific volume and flexibility increase, during storage of bread, water retention. Water and anti-aging effect of significantly improved.%将魔芋萄甘聚糖和卡拉胶、大豆分离蛋白复配,研究其对面团粉质、拉伸特性和面包理化、感官指标的影响.结果表明:增加魔芋葡甘聚糖,减少卡拉胶添加量,能引起面团的吸水率、形成时间、拉伸比数增加;拉伸能量、延伸性减小,面团的评价值先增加后降低.大豆分离蛋白在一定添加量时,会增加吸水率,延长形成时间.魔芋葡甘聚糖和卡拉胶、大豆分离蛋白添加到面粉的最佳比例为2;1;25,当面粉中复配胶添加量达3%时,面团调制时网络结构增强,面包含水量、比容和弹性增加,面包储存过程中,持水、保水和抗老化效果明显改善.

  5. Monday effect

    OpenAIRE

    Vicent Almela, Vicente

    2014-01-01

    Treball final de Grau en Economia. Codi: EC1049. Curs acadèmic 2013-2014 Monday effect consists of fuel price falling between Monday and Sunday, followed by price increases during the following days. It is coincidentally on Monday when the European Commission collects fuel prices data to be able to elaborate statistics. In this essay we have firstly studied which has been the recent evolution of the Monday effect in our country. After that we have analysed the magnitude and the...

  6. Treatment Effects

    DEFF Research Database (Denmark)

    Heckman, James J.; Lopes, Hedibert F.; Piatek, Rémi

    2014-01-01

    This paper contributes to the emerging Bayesian literature on treatment effects. It derives treatment parameters in the framework of a potential outcomes model with a treatment choice equation, where the correlation between the unobservable components of the model is driven by a low...... to observe the same person in both the treated and untreated states, but it also turns out to be straightforward to implement. Formulae are provided to compute mean treatment effects as well as their distributional versions. A Monte Carlo simulation study is carried out to illustrate how the methodology can...

  7. Ventilation Effectiveness

    DEFF Research Database (Denmark)

    Mundt, M.; Mathisen, H. M.; Moser, M.;

    Improving the ventilation effectiveness allows the indoor air quality to be significantly enhanced without the need for higher air changes in the building, thereby avoiding the higher costs and energy consumption associated with increasing the ventilation rates. This Guidebook provides easy...

  8. Natural Marine and Synthetic Xenobiotics Get on Nematode’s Nerves: Neuro-Stimulating and Neurotoxic Findings in Caenorhabditis elegans

    Directory of Open Access Journals (Sweden)

    Thora Lieke

    2015-05-01

    Full Text Available Marine algae release a plethora of organic halogenated compounds, many of them with unknown ecological impact if environmentally realistic concentrations are applied. One major compound is dibromoacetic acid (DBAA which was tested for neurotoxicity in the invertebrate model organism Caenorhabditis elegans (C. elegans. This natural compound was compared with the widespread synthetic xenobiotic tetrabromobisphenol-A (TBBP-A found in marine sediments and mussels. We found a neuro-stimulating effect for DBAA; this is contradictory to existing toxicological reports of mammals that applied comparatively high dosages. For TBBP-A, we found a hormetic concentration-effect relationship. As chemicals rarely occur isolated in the environment, a combination of both organobromines was also examined. Surprisingly, the presence of DBAA increased the toxicity of TBBP-A. Our results demonstrated that organohalogens have the potential to affect single organisms especially by altering the neurological processes, even with promoting effects on exposed organisms.

  9. [Gamma-radiation action on cells of algae Euglena gracilis].

    Science.gov (United States)

    Glinkova, E; Zhuchkina, N I; Koltovoĭ, N A; Koltovaia, N A

    2012-01-01

    Considering the potentials of algae Euglena to constitute a part of biological systems of human life support, effects of low radiation doses on algal cells and radiosensitivity dependence on their genotype were studied. In experiments with gamma-irradiation (60Co) of Euglena gracilis, the highest radioresistance was demonstrated by strain Z. OFL; the chloroplasts lacking Z-derived strain showed hypersensitivity to radiation. E. bacillaris and derived chlorophyll-lacking strains W3 and W10 had intermediate radiosensitivity. Irradiation with the doses of up to 10 Gy produced a hormetic effect in the stock strains. Cells death was observed only after irradiation by doses above 100 Gy. The stimulating effect was exerted both on radioresistance and growth rate. Dyes made possible rapid evaluation of the proportion of living and dead cells. Comparison of two survival tests showed that the classic medium inoculation overestimates cell deaths as it disregards the living non-proliferating cells.

  10. Radiation vulcanization of natural rubber latex (part 6). Improvement of aging propreties of latex lubber vulcanized with radiation

    Energy Technology Data Exchange (ETDEWEB)

    Makuuchi, Keizo; Tsushima, Kyogo

    1988-10-15

    Concerning the vulcanization of natural rubber latex with radiation by using acrylic ester as accelerator, causes for loss of anti-aging property by leaching and counter-measure against it were reported. For the experiment, 2-ethyl hexyl acrylate with carbon tetra-chloride as accelerator, 2% aqueous solution of ammonia or acetic acid as leaching water, were used. For the analysis of extracts, ultra-violet absorption and gas chromatography were applied. As the result of the experiment, it was recognized that anti-aging property was more decreased by more increased quantity of extracted protein which was naturally contained in the natural rubber latex and functioned as anti-aging material, and that by radiation vulcanization, non-rubber components were destroyed to result the increased quantity of extracts compared with that of non-vulcanized. Concerning the improvement of anti-aging property, experimental comparison of miscellaneous methods resulted that the addition of sodium di-n-buthyl di-thiocarbamate was most effective. 3 references, 3 figures, 5 tables.

  11. Wound Healing Activity of Topical Application Forms Based on Ayurveda

    Directory of Open Access Journals (Sweden)

    Hema Sharma Datta

    2011-01-01

    Full Text Available The traditional Indian medicine—Ayurveda, describes various herbs, fats, oils and minerals with anti-aging as well as wound healing properties. With aging, numerous changes occur in skin, including decrease in tissue cell regeneration, decrease in collagen content, loss of skin elasticity and mechanical strength. We prepared five topical anti-aging formulations using cow ghee, flax seed oil, Phyllanthus emblica fruits, Shorea robusta resin, Yashada bhasma as study materials. For preliminary efficacy evaluation of the anti-aging activity we chose excision and incision wound healing animal models and studied the parameters including wound contraction, collagen content and skin breaking strength which in turn is indicative of the tissue cell regeneration capacity, collagenation capacity and mechanical strength of skin. The group treated with the formulations containing Yashada bhasma along with Shorea robusta resin and flax seed oil showed significantly better wound contraction (P < .01, higher collagen content (P < .05 and better skin breaking strength (P < .01 as compared to control group; thus proposing them to be effective prospective anti-aging formulations.

  12. Tectonomagnetic effects

    Science.gov (United States)

    Johnston, M.

    1978-01-01

    Measurements of the actual stress within the Earth and its changes with time are very difficult. It is much easier to monitor the direct effects of this stress, such as ground strain, or the indirect effects, such as changes in resistivity, strain, changes in seismic velocity or changes in magnetic field, and so forth. The latter technique is one of the more promising methods for monitoring substantial volumes of the Earth's crust near active faults with only a few instruments. It derives from the piezomagnetic properties of rocks (that is, the change in rock magnetization and, therefore, local magnetic field due to a change in applied stress). AS stress and strain fields along active faults vary, these variations should be reflected in changing local magnetic fields. In particular, rapidly changing fields might be expected to occur just before a large earthquake.

  13. Health effects

    Energy Technology Data Exchange (ETDEWEB)

    Mahieu, L

    1998-07-01

    The main objectives of research in the field of health effects at the Belgian Nuclear Research Centre SCK-CEN are: (1) to study cancer mortality and morbidity in nuclear workers in Belgium; (2) to document the feasibility of retrospective cohort studies in Belgium; (3) to participate in the IARC study; (4) to elucidate the mechanisms of the effects of ionizing radiation on the mammalian embryo during the early phases of its development; (5) to assess the genetic risks of material exposure to ionizing radiation; (6) to elucidate the cellular mechanisms leading to brain damage after prenatal irradiation; (7) to advise authorities and to provide the general population with adequate information concerning the health risk arising from radiation exposure. Progress and major achievements in these topical areas for 1997 are reported.

  14. AFTER EFFECTS

    Institute of Scientific and Technical Information of China (English)

    2004-01-01

    创建物体动画最快速的方法是使用运动捕捉,这是一种以真实世界物体为动画而提供的运动。现在打开光盘After Effects Shortcut文件夹中的文件,依照我们循序渐进的技巧教程来学习如何使用这种方法。

  15. Ventilation effectiveness

    CERN Document Server

    Mathisen, Hans Martin; Nielsen, Peter V; Moser, Alfred

    2004-01-01

    Improving the ventilation effectiveness allows the indoor air quality to be significantly enhanced without the need for higher air changes in the building, thereby avoiding the higher costs and energy consumption associated with increasing the ventilation rates. This Guidebook provides easy-to-understand descriptions of the indices used to mesure the performance of a ventilation system and which indices to use in different cases.

  16. The organic air pollutant cumene hydroperoxide interferes with NO antioxidant role in rehydrating lichen.

    Science.gov (United States)

    Catalá, M; Gasulla, F; Pradas Del Real, A E; García-Breijo, F; Reig-Armiñana, J; Barreno, E

    2013-08-01

    Organic pollutants effects on lichens have not been addressed. Rehydration is critical for lichens, a burst of free radicals involving NO occurs. Repeated dehydrations with organic pollutants could increase oxidative damage. Our aim is to learn the effects of cumene hydroperoxide (CP) during lichen rehydration using Ramalina farinacea (L.) Ach., its photobiont Trebouxia spp. and Asterochloris erici. Confocal imaging shows intracellular ROS and NO production within myco and phycobionts, being the chloroplast the main source of free radicals. CP increases ROS, NO and lipid peroxidation and reduces chlorophyll autofluorescence, although photosynthesis remains unaffected. Concomitant NO inhibition provokes a generalized increase of ROS and a decrease in photosynthesis. Our results suggest that CP induces a compensatory hormetic response in Ramalina farinacea that could reduce the lichen's antioxidant resources after repeated desiccation-rehydration cycles. NO is important in the protection from CP.

  17. Effective Teachers

    Directory of Open Access Journals (Sweden)

    Beverly A. King Miller

    2015-09-01

    Full Text Available This article focuses on the educational strategies that can be used to support female students of African descent in their persistence in science, technology, engineering, and mathematics (STEM education and careers. STEM careers have historically been White male and White female dominated, which has yielded an underrepresentation of those of African descent. Drawing from a grounded qualitative case study, the data used for this article share the responses of Afro-Caribbean females in STEM who have immigrated to the United States from the country of Panama. As Latinas, they are representative of the changing face in the American educational system—bilingual, multicultural, and of African descent. The strategies offered reflect their own teaching practices, their former teachers, or experiences with their children’s teachers. What emerged were descriptions of four strategies and behaviors of effective teachers that align with Ladson-Billings’s culturally relevant pedagogy and Gay’s culturally responsive teaching. Included in the findings are the high standards and expectations embodied by effective teachers that serve to positively inspire their students. Culturally responsive teachers create an atmosphere of learning that supports academic success, conveying their belief in their students’ ability based upon their own reflectivity. As the U.S. educational system continues to become multilingual and multicultural, there is need for strategies for the successful inclusion and progression of students in STEM educational pathways and careers. This will occur as teachers challenge themselves to be the agents of change in the lives of their students.

  18. Effects of

    Directory of Open Access Journals (Sweden)

    S. Gowthaman

    2016-03-01

    Full Text Available This work investigates the effect of both inlet air temperature and fuel injection pressure on performance and emission behaviour of homogeneous charge compression ignition engine (HCCI fuelled with diesel fuel. In this investigation, HCCI engine operates with different inlet air temperature and fuel injection pressure, and analysis the effect of these variables on HCCI engine performance and emissions. The inlet air temperatures are varied between 40 °C and 70 °C and the injection pressure in the port fuel injector is varied from 3 bar to 5 bar respectively. From the results, the optimum inlet air temperature and fuel injection pressure for efficient HCCI engine operation are identified. The result shows that, brake thermal efficiency of HCCI is nearer to the value of conventional diesel engine, and can be obtained if HCCI engine operates with 5 bar injection pressure and 60 °C air temperature and a simultaneous reduction in oxides of nitrogen (NOx and smoke emissions compared to conventional diesel engine. However, when inlet air is heated for improvement of vaporisation of diesel fuel, the higher inlet air temperature limits the operation range of HCCI engine, due to high knocking intensity, high NOx emissions and misfire of charge. The fuel injection pressure is also limited due to high level of HC and NOx emissions.

  19. The Synthetic Elicitor 2-(5-Bromo-2-Hydroxy-Phenyl)-Thiazolidine-4-Carboxylic Acid Links Plant Immunity to Hormesis.

    Science.gov (United States)

    Rodriguez-Salus, Melinda; Bektas, Yasemin; Schroeder, Mercedes; Knoth, Colleen; Vu, Trang; Roberts, Philip; Kaloshian, Isgouhi; Eulgem, Thomas

    2016-01-01

    Synthetic elicitors are drug-like compounds that induce plant immune responses but are structurally distinct from natural defense elicitors. Using high-throughput screening, we previously identified 114 synthetic elicitors that activate the expression of a pathogen-responsive reporter gene in Arabidopsis (Arabidopsis thaliana). Here, we report on the characterization of one of these compounds, 2-(5-bromo-2-hydroxy-phenyl)-thiazolidine-4-carboxylic acid (BHTC). BHTC induces disease resistance of plants against bacterial, oomycete, and fungal pathogens and has a unique mode of action and structure. Surprisingly, we found that low doses of BHTC enhanced root growth in Arabidopsis, while high doses of this compound inhibited root growth, besides inducing defense. These effects are reminiscent of the hormetic response, which is characterized by low-dose stimulatory effects of a wide range of agents that are toxic or inhibitory at higher doses. Like its effects on defense, BHTC-induced hormesis in Arabidopsis roots is partially dependent on the WRKY70 transcription factor. Interestingly, BHTC-induced root hormesis is also affected in the auxin-response mutants axr1-3 and slr-1. By messenger RNA sequencing, we uncovered a dramatic difference between transcriptional profiles triggered by low and high doses of BHTC. Only high levels of BHTC induce typical defense-related transcriptional changes. Instead, low BHTC levels trigger a coordinated intercompartmental transcriptional response manifested in the suppression of photosynthesis- and respiration-related genes in the nucleus, chloroplasts, and mitochondria as well as the induction of development-related nuclear genes. Taken together, our functional characterization of BHTC links defense regulation to hormesis and provides a hypothetical transcriptional scenario for the induction of hormetic root growth.

  20. Assessment of landfill leachate toxicity reduction after biological treatment.

    Science.gov (United States)

    Jemec, Anita; Tišler, Tatjana; Zgajnar-Gotvajn, Andreja

    2012-02-01

    In the present article, the efficiency of biological treatment of landfill leachates was evaluated by implementation of physicochemical characterisation and a complex toxicity assessment. An array of toxicity tests using bacterium Vibrio fischeri, alga Desmodesmus subspicatus, crustacean Daphnia magna, and embryo of fish Danio rerio, as well as unconventional methods using biochemical biomarkers (protein content, enzymes cholinesterase, and glutathione-S-transferase), were employed. Toxicity of leachates varied depending on the season of collection in relation to their different physicochemical characteristics. Uncommon effects of leachates on organisms, such as hormetic-like increases of algal growth and reproduction of daphnids, were identified. New approaches using the activities of enzymes were found unsuitable for routine hazard assessment of leachates. Although physicochemical parameters and toxicity decreased significantly after biological treatment, the effluents did not meet the demands of the current Slovenian legislation; thus, the existing biological treatment was found inappropriate. The development of advanced treatment techniques for landfill leachates is thus encouraged.

  1. Lactobacilli Modulate Epithelial Cytoprotection through the Nrf2 Pathway.

    Science.gov (United States)

    Jones, Rheinallt M; Desai, Chirayu; Darby, Trevor M; Luo, Liping; Wolfarth, Alexandra A; Scharer, Christopher D; Ardita, Courtney S; Reedy, April R; Keebaugh, Erin S; Neish, Andrew S

    2015-08-25

    An optimal gut microbiota influences many beneficial processes in the metazoan host. However, the molecular mechanisms that mediate and function in symbiont-induced host responses have not yet been fully characterized. Here, we report that cellular ROS enzymatically generated in response to contact with lactobacilli in both mice and Drosophila has salutary effects against exogenous insults to the intestinal epithelium via the activation of Nrf2 responsive cytoprotective genes. These data show that the xenobiotic-inducible Nrf2 pathway participates as a signaling conduit between the prokaryotic symbiont and the eukaryotic host. Indeed, our data imply that the capacity of lactobacilli to induce redox signaling in epithelial cells is a highly conserved hormetic adaptation to impel cellular conditioning to exogenous biotic stimuli. These data also highlight the role the microbiota plays in eukaryotic cytoprotective pathways and may have significant implications in the characterization of a eubiotic microbiota.

  2. OXIDATIVE STRESS IN MUSCLE GROWTH AND ADAPTATION TO PHYSICAL EXERCISE

    Directory of Open Access Journals (Sweden)

    Ihor Yurkevych

    2015-05-01

    Full Text Available In a few last decades oxidative stress detected in a variety of physiological processes where reactive oxygen species (ROS and reactive nitrogen species (RNS play a central role. They are directly involved in oxidation of proteins, lipids and nucleic acids. In certain concentrations they are necessary for cell division, proliferation and apoptosis. Contractile muscle tissue at aerobic conditions form high ROS flow that may modulate a variety of cell functions, for example proliferation. However, slight increase in ROS level provide hormetic effect which may participate in adaptation to heavy weight training resulted in hypertrophy and proliferation of skeletal muscle fibers. This review will discuss ROS types, sites of generation, strategies to increase force production and achieve skeletal muscle hypertrophy.

  3. Hormesis, allostatic buffering capacity and physiological mechanism of physical activity: a new theoretic framework.

    Science.gov (United States)

    Li, Guolin; He, Hong

    2009-05-01

    Despite great progress made in sports medicine, the physiological mechanism of moderate physical activity-induced physical fitness remains only partly understood. Combined with the hormetic characteristic of physical activity and property of allostasis, we first propose the hormesis induced allostatic buffering capacity enhancement as a physiological mechanism to explain the moderate physical activity-induced physical fitness. As stressful stimulus, physical activity can induce several stresses in the host, including eustress ('good stress') and distress ('bad stress'), which may have both positive and negative effects. Too little or too much physical activities will introduce too weak eustress or too strong distress and result in allostasis load through weakening allostatic buffering capacity or damaging allostatic buffering capacity respectively. However, moderate physical activities will introduce eustress and contribute to the hormesis induced allostatic buffering capacity enhancement, which benefits organism.

  4. Caprylic acid in the effective treatment of intractable medical problems of frequent urination, incontinence, chronic upper respiratory infection, root canalled tooth infection, ALS, etc., caused by asbestos & mixed infections of Candida albicans, Helicobacter pylori & cytomegalovirus with or without other microorganisms & mercury.

    Science.gov (United States)

    Omura, Yoshiaki; O'Young, Brian; Jones, Marilyn; Pallos, Andrew; Duvvi, Harsha; Shimotsuura, Yasuhiro

    2011-01-01

    application for anti-cancer, anti-aging, anti-Alzheimer's disease, anti-Autism, anti-infection, & general circulatory improvement.

  5. Effect of Schisandra chinensis polysaccharide on intracerebral acetylcholinesterase and monoamine neurotransmitters in a D-galactose-induced aging brain mouse model

    Institute of Scientific and Technical Information of China (English)

    Mingsan Miao; Jianlian Gao; Guangwei Zhang; Xiao Ma; Ying Zhang

    2009-01-01

    BACKGROUND: The most prominent characteristic of brain aging is decreased learning and memory ability. The functions of learning and memory are closely related to intracerebral acetylcholinesterase (ACHE) and monoamine neurotransmitter activity. Previous studies have shown that Schisandra chinensis potysaccharide has an anti-aging effect. OBJECTIVE: To explore the effects of Schisandra chinensis polysaccharide on AChE activity and monoamine neurotransmitter content, as well as learning and memory ability in a D-galactose-induced aging mouse brain model compared with the positive control drug Kangnaoling. DESIGN, TIME AND SETTING: Completely randomized, controlled experiment based on neurobiochemistry was performed at the Pharmacological Laboratory, Henan University of Traditional Chinese Medicine from September to December 2003.MATERIALS: Schisandra chinensis was purchased from Henan Provincial Medicinal Company. Schisandra chinensis polysaccharide was obtained by water extraction and alcohol precipitation. Kangnaoling pellets were provided by Liaoning Tianlong Pharmaceutical (batch No. 20030804;state drug permit No. H21023095). A total of 50 six-week-old Kunming mice were randomly divided into five groups: blank control, model, Kangnaoling, high and low dosage Schisandra chinensis polysaccharide groups, with 10 mice per group. METHODS: Mice in the blank control group were subcutaneously injected with 0.5 mL/20 g normal saline into the nape of the neck each day, while the remaining mice were subcutaneously injected with 5% D-galactose saline solution (0.5 mL/20 g) in the nape for 40 days to induce a brain aging model. On day 11, mice in the high and low dosage Schisandra chinensis polysaccharide groups were intragastrically infused with 20 mg/mL and 10 mg/mL Schisandra chinensis polysaccharide solution (0.2 mL/10 g), respectively. Mice from the Kangnaoling group were intragastrically infused with 35 mg/mL Kangnaoling suspension (0.2 mL/10 g), and the mice in the

  6. Maca (Lepidium meyenii WALP), a review of its biological properties

    OpenAIRE

    Gonzales, Gustavo F.; Facultad de Ciencias y Filosofía, Universidad Peruana Cayetano Heredia. Lima, Perú. Instituto de Investigaciones de la Altura, Universidad Peruana Cayetano Heredia. Lima, Perú. Doctor en Medicina y doctor en Ciencias.; Villaorduña, Leonidas; Universidad Nacional Daniel Alcides Carrión. Cerro de Pasco, Perú. doctor en Economía.; Gasco, Manuel; Facultad de Ciencias y Filosofía, Universidad Peruana Cayetano Heredia. Lima, Perú. Instituto de Investigaciones de la Altura, Universidad Peruana Cayetano Heredia. Lima, Perú. magíster en Ciencias.; Rubio, Julio; Facultad de Ciencias y Filosofía, Universidad Peruana Cayetano Heredia. Lima, Perú. licenciado en Biología.; Gonzales, Carla; Facultad de Ciencias y Filosofía, Universidad Peruana Cayetano Heredia. Lima, Perú. Instituto de Investigaciones de la Altura, Universidad Peruana Cayetano Heredia. Lima, Perú. doctora en Ciencias.

    2014-01-01

    Maca (Lepidium meyenii) is a plant that grows above 4000 altitude meters in Peru’s Central Andes; it has different varieties according to the color of the hypocotyl. This review summarizes the results of studies about the effects of maca on sexual function, spermatogenesis, female reproductive function, memory, depression and anxiety, and energy as well as effects on benign prostatic hyperplasia, osteoporosis and metabolic syndrome. Its anti-aging effect is also discussed as well as safety in...

  7. dermaOXY skin assay: effect and evidence

    DEFF Research Database (Denmark)

    Menov, Lasse; Klösgen-Buchkremer, Beate Maria

    2015-01-01

    This text is a videnkupon report supported by the Danish Innovation Fonds and conducted by L.M. and B.K. for dermaOXY (by MedicTinedic ApS, Varde, Denmark). It involves two dermaOXY products: dermaOXY HYALURON SERUM and dermaOXY SYN SERUM. These are applied to the facial skin in combination...... with a 90 percent pure oxygen gas stream. Occasionally, the treatment is supported by low-level light exposure, prepared by mechanical microporation of skin or both. The dermaOXY skin improvement approach is used in treatments by clinics spread across 23 countries [1]. This text also includes an assessment...... of the instrument set DermaLab®Combo, which is used for the physical characterization of skin status after treatment. The report consists of four main parts, dedicated to 1. the properties of human skin 2. the anti-aging methods applied by the dermaOXY treatment 3. the analytical methods applied by derma...

  8. Differential translocation of heat shock factor-1 after mild and severe stress to human skin fibroblasts undergoing aging in vitro.

    Science.gov (United States)

    Demirovic, Dino; de Toda, Irene Martinez; Nizard, Carine; Rattan, Suresh I S

    2014-12-01

    Repeated exposure to mild heat shock (HS) has been shown to induce a wide range of health promoting hormetic effects in various biological systems, including human cells undergoing aging in vitro. In order to understand how cells distinguish between mild and severe stress, we have investigated the extent of early and immediate HS response by analyzing the nuclear translocation of the transcription factor heat shock factor-1 (HSF1), in serially passaged normal adult human facial skin fibroblasts exposed to mild (41 °C) or severe (43 °C) HS. Cells respond differently when exposed to mild and severe HS at different passage levels in terms of the extent of HSF1 translocation. In early passage young cells there was a 5-fold difference between mild and severe HS in the extent of HSF1 translocation. However, in near senescent late passage cells, the difference between mild and severe stress in terms of the extent of HSF1 translocation was reduced to less than 2-fold. One of the reasons for this age-related attenuation of heat shock response is due to the fact there was a higher basal level of HSF1 in the nuclei of late passage cells, which is indicative of increased intrinsic stress during cellular aging. These observations are consistent with previously reported data that whereas repeated mild stress given at younger ages can slow down aging and increase the lifespan, the same level of stress given at older ages may not provide the same benefits. Therefore, elucidating the early and immediate steps in the induction of stress response can be useful in deciding whether a particular level of stress is potentially hormetically beneficial or not.

  9. 从“药食同源”探讨抗衰老中药激活AMPK通路改善能量代谢的潜在机制%Anti-aging traditional Chinese medicine: potential mechanisms involving AMPK pathway and calorie restriction based on "medicine-food homology" theory

    Institute of Scientific and Technical Information of China (English)

    刘东岩; 李亦晗; 徐砚通; 朱彦

    2016-01-01

    关于中药的抗衰老功效,已经有许多相关报道,但是对于其物质基础及作用机制尚未明确阐明.腺苷酸活化蛋白激酶(AMP-activated protein kinase,AMPK)是能量代谢的感受器,能够延长寿命这一效应,在不同实验中都得到相应的证实.过表达或用二甲双胍激活AAK-2/AMPK均有延长线虫、果蝇寿命的功效.AMPK抗衰老的可能下游通路包括TOR/S6k途径、FOXOs途径、CRTC途径等.中医药核心理念之一为治未病,其表现之一即是以药食同源方式改善机体,由此达到长寿的目的.部分药食同源药物有可能通过激活AMPK以达到养生,延长寿命的目的.经过总结发现,相关中药复方中丹参、黄芪、黄连、茯苓、白术、大黄、人参使用频率较高,并且补虚活血类药物占主导.采用ingenuity pathway analysis (IPA)网络药理学软件分析,间接作用于AMPK靶蛋白而非其上下游通路的单体药物有黄连素、大黄素、姜黄素、白藜芦醇、虫草素、牛蒡子苷元等6个,在一定程度上说明药食同源药物通过AMPK通路延长寿命的可行性.该文综合数据库查询和IPA网络药理学软件分析对具有激活AMPK通路来延缓衰老功能的中药单体及组分进行了归纳和总结,并基于中医药“药食同源”的理念将这些药物可能通过改善能量代谢对于延缓衰老的影响进行了展望.

  10. 以秀丽隐杆线虫为模式生物的蓝莓果酒抗氧化及抗衰研究%Study on antioxidant and anti-aging analysis of blueberry wine in C.elegans

    Institute of Scientific and Technical Information of China (English)

    魏焘; 曾敏; 李珂; 王远亮

    2016-01-01

    为探讨不同组分的蓝莓果酒对秀丽隐杆线虫抗氧化性能与抗细胞凋亡的影响,从而确定蓝莓果酒中主要物质的浓度及食用功能特性.以秀丽隐杆线虫(Caenorhabditis elegans)为模式生物,a组在蓝莓果酒的酒精度(体积分数)为14%的基础上,通过改变其原花青素(proant hocyanidin,PC)含量设置6个喂食组,b组在蓝莓果酒的原花青素含量为3 mg/mL的基础上,通过改变其酒精度设置7个喂食组,对照组线虫以大肠杆菌OP50喂养,检测各组线虫的抗氧化酶活力以及细胞凋亡情况.结果表明:蓝莓酒中的原花青素是延缓衰老的有效成分,低浓度酒精(<10%)也能延缓衰老,但是当酒精度(体积分数)高于10%时,则具有反作用,原花青素浓度为3 mg/mL、酒精度(体积分数)为10%的蓝莓果酒对线虫的抗衰老作用最佳.

  11. 纳米Cu-TiO2在亲水涂料中的耐老化和抗菌性能研究%The Anti- Aging and Antibacterial Properties of Nano Cu -TiO2 in Hydrophilic Coatings

    Institute of Scientific and Technical Information of China (English)

    罗俊旋; 徐游; 曾伟华; 汤皎宁

    2011-01-01

    In this paper, the photocatalytic reduction method was used for preparation of nano copper - titanium dioxide (Cu/TiO2)antibacterial agent. Prime aluminum foil was coated with antimicrobial coating containing 1.4% antimicrobial agent using dipping process. An accelerated ageing treatment on the antimicrobial coatings was conducted. Contact angles tester, metallurgical microscope, stereomicroscope and antibacterial test were used to measure the change of contact angle, surface morphology, and antibacterial performance of coatings before and after ageing. The results showed that the antimicrobial coating with 1.4% antimicrobial agent has excellent bactericidal properties, hydrophilic properties and ageing resistance. The antimicrobial rate is 99.9% before ageing,and after ageing treatment, the average antibacterial rate is more than 95%, while hydrophilic angle is kept less than 8°.%通过光催化还原的方法制备了纳米铜基二氧化钛(Cu-TiO2)抗菌剂.使用浸涂方法将素铝箔基片涂敷上含1.4%抗菌剂的面漆抗菌涂层.对抗菌涂层进行加速老化处理,采用接触角仪、金相显微镜、体视显微镜、抗菌实验等测定涂层老化前后的接触角、表面形貌以及抗菌性能的变化.结果表明:含1.4%抗菌剂的抗菌涂层具有优秀的杀菌性能、亲水性能和耐老化性能.老化之前抗菌率达99.9%以上,老化处理后,涂层表面无明显变化,平均抗菌率达到95%以上,同时亲水角保持小于8°.

  12. 有氧运动对人全基因组表达的影响%Effect of aerobic exercise on genome expression in human skeletal muscle

    Institute of Scientific and Technical Information of China (English)

    黄碧燕; 柯杰兵; 张翔; 傅忠义; 姜立; 孙毅; 王王

    2009-01-01

    OBJECTIVE: To evaluate the effect of aerobic exercise on genome expression in human skeletal muscle. METHODS: Six healthy sedentary elderly men aged (66+9) years were selected from military cadre retirement centers and after exercise. Testing indices included height, weight, vital capacity, step index and maximal oxygen uptake. Needle biopsies were obtained from the skeletal muscle before and after the last training. Total RNA extracted from the samples was hybridized to Affymetrix U 133A platform, the gene expression datum was analyzed.RESULTS: Aerobic exercise was shown to improve cardiorespiratory function and reduce body fat of elder subjects. It could alter the genome expression in human skeletal muscle, the number of genes that passed filtering criteria was 725. The most differently expressed genes (n=20) were investigated in this study, in which there were 3 upregulated and 17 downregulated. According to gene function annotations, the differential genes were classified into 8 categories which concerned cellular component and biological process, Kyoto Encyclopedia of Genes and Genomes (KEGG) searching showed 4 genes' metabolism pathway. CONCLUSION: Systematic aerobic exercise upregulates expression of enzyme genes concerning tricarboxylic acid cycle, and downregulates expression of genes conceming muscle protein synthesis and sphingolipid. It is suggested that aerobic exercise is good to protect human nerves' integrity, exerts positive action on anti-aging and accelerate the aerobic metabolism of lipid materials in vivo.%目的:观察有氧运动对骨骼肌全基因组表达的影响.方法:选择6名某部队干休所中很少运动、年龄(66±9)岁的健康老年人集进行为期12周太极拳训练.运动前和运动12周后,所有受试都进行了体质评估.测试指标包括身高、体质量、肺活量、台阶指数、最大摄氧量.在训练前后分别对实验对象进行肌活验,提取总RNA,经处理后与Affymetdx U133A基因芯片进

  13. Physiological effects in aromatherapy

    OpenAIRE

    2004-01-01

    The effects of aromas on humans are divided into physiological and psychological effects. The physiological effect acts directly on the physical organism, the psychological effect acts via the sense of smell or olfactory system, which in turn may cause a physiological effect. This paper reviews on the physiological effects which are used for the evaluation of the effects of aromas. Physiological parameters, i.e. heart rate blood pressure, electrodermal activity, electroencephalogram, slow pot...

  14. Polyphenols as active ingredients for cosmetic products.

    Science.gov (United States)

    Zillich, O V; Schweiggert-Weisz, U; Eisner, P; Kerscher, M

    2015-10-01

    Polyphenols are secondary plant metabolites with antioxidant, anti-inflammatory and anti-microbial activity. They are ubiquitously distributed in the plant kingdom; high amounts contain, for example, green tea and grape seeds. Polyphenolic extracts are attractive ingredients for cosmetics and pharmacy due to their beneficial biological properties. This review summarizes the effects of polyphenols in the context of anti-ageing activity. We have explored in vitro studies, which investigate antioxidant activity, inhibition of dermal proteases and photoprotective activity, mostly studied using dermal fibroblasts or epidermal keratinocytes cell lines. Possible negative effects of polyphenols were also discussed. Further, some physicochemical aspects, namely the possible interactions with emulsifiers and the influence of the cosmetic formulation on the skin delivery, were reported. Finally, few clinical studies, which cover the anti-ageing action of polyphenols on the skin after topical application, were reviewed.

  15. Supercritical Fluid Extraction of Saponins from Ginseng

    Institute of Scientific and Technical Information of China (English)

    LIU Chun-ming; ZHAO Xue-liang; LI Hong-mei; LIU Zhi-qiang; XING Jun-peng

    2005-01-01

    @@ Introduction Ginseng( Panax ginseng C. A. Meyer, Araliaceae) is one of the most valuable Chinese crude drugs and has been used widely for over 2000 years. Studies have demonstrated that ginseng can act on the central nervous system, the cardiovascular system and the endocrine system; it can enhance immune function and metabolism; it possesses a biomodulation action, anticancer effect, anti-stress and anti-ageing activities, and so on[1-8].

  16. Physiological effects in aromatherapy

    Directory of Open Access Journals (Sweden)

    Tapanee Hongratanaworakit

    2004-01-01

    Full Text Available The effects of aromas on humans are divided into physiological and psychological effects. The physiological effect acts directly on the physical organism, the psychological effect acts via the sense of smell or olfactory system, which in turn may cause a physiological effect. This paper reviews on the physiological effects which are used for the evaluation of the effects of aromas. Physiological parameters, i.e. heart rate blood pressure, electrodermal activity, electroencephalogram, slow potential brain waves (contingent negativevariation, and eye blink rate or pupil functions, are used as indices for the measurement of the aroma effects

  17. Historical blunders: how toxicology got the dose-response relationship half right.

    Science.gov (United States)

    Calabrese, E J

    2005-12-14

    Substantial evidence indicates that reliable examples of hormetic dose responses in the toxicological literature are common and generalizable across biological model, endpoint measured and chemical class. Further evaluation revealed that the hormetic dose response model is more common than the threshold dose response model in objective, head-to-head comparisons. Nonetheless, the field of toxicology made a profound error by rejecting the use of the hormetic dose response model in its teaching, research, risk assessment and regulatory activities over nearly the past century. This paper argues that the hormetic dose response model (formerly called the Arndt-Schulz Law) was rejected principally because of its close historical association with the medical practice of homeopathy as a result of the prolonged and bitter feud between traditional medicine and homeopathy. Opponents of the concept of hormesis, making use of strong appeals to authority, were successful in their misrepresentation of the scientific foundations of hormesis and in their unfair association of it with segments of the homeopathic movement with extreme and discreditable views. These misrepresentations became established and integrated within the pharmacology and toxicology communities as a result of their origins in and continuities with traditional medicine and subsequently profoundly impacted a broad range of governmental risk assessment activities further consolidating the rejection of hormesis. This error of judgment was reinforced by toxicological hazard assessment methods using only high and few doses that were unable to assess hormetic responses, statistical modeling processes that were constrained to deny the possibility of hormetic dose response relationships and by the modest nature of the hormetic stimulatory response itself, which required more rigorous study designs to evaluate possible hormetic responses.

  18. Synthesis and characterization of organic intercalated layered double hydroxides and their application in bitumen modification

    Energy Technology Data Exchange (ETDEWEB)

    Xu, Song [State Key Laboratory of Silicate Materials for Architectures, Wuhan University of Technology, Wuhan 430070 (China); Yu, Jianying, E-mail: jyyu@whut.edu.cn [State Key Laboratory of Silicate Materials for Architectures, Wuhan University of Technology, Wuhan 430070 (China); Sun, Yubin [Center for Materials Research and Analysis, Wuhan University of Technology, Wuhan 430070 (China); Wu, Shaopeng [State Key Laboratory of Silicate Materials for Architectures, Wuhan University of Technology, Wuhan 430070 (China)

    2015-02-15

    Organic layered double hydroxides (LDHs) intercalated by sodium dodecylbenzenesulfonate (SDBS) were prepared by anion-exchange method and applied to modify bitumen aiming at improving ageing resistance of bitumen. The organic LDHs (SDBS–LDHs) were characterized by X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM) and Ultraviolet and visible (UV–vis) spectrophotometry. The effect of SDBS–LDHs and LDHs on physical and anti-ageing properties of bitumen was evaluated by means of conventional and rheological test. The results of XRD, FTIR and SEM show that SDBS is successfully intercalated into interlayer of LDHs, and the UV–vis reflectance and absorbance curves illustrate that intercalation of SDBS enhances the UV shielding effect of LDHs. The addition of SDBS–LDHs or LDHs has little influence on physical properties of bitumen because SDBS–LDHs and LDHs are physically mixed in bitumen. Compared with pristine bitumen after TFOT and UV irradiation ageing, the introduction of SDBS–LDHs and LDHs significantly improves thermal- and photo-oxidative ageing resistance of bitumen. Notably, bitumen with SDBS–LDHs exhibits better anti-ageing performance than that with LDHs, implying more effective modification of SDBS-LDHs which is due to the enhanced UV protective ability and compatibility with bitumen of SDBS–LDHs. - Highlights: • XRD, FTIR and SEM were used to confirm the successful intercalation. • SDBS–LDHs show superior UV protective ability. • SDBS–LDHs improved the anti-ageing properties of bitumen.

  19. On Effect Size

    Science.gov (United States)

    Kelley, Ken; Preacher, Kristopher J.

    2012-01-01

    The call for researchers to report and interpret effect sizes and their corresponding confidence intervals has never been stronger. However, there is confusion in the literature on the definition of effect size, and consequently the term is used inconsistently. We propose a definition for effect size, discuss 3 facets of effect size (dimension,…

  20. GDF11 administration does not extend lifespan in a mouse model of premature aging

    Science.gov (United States)

    Freitas-Rodríguez, Sandra; Rodríguez, Francisco; Folgueras, Alicia R.

    2016-01-01

    GDF11 has recently emerged as a powerful anti-aging candidate, found in young blood, capable of rejuvenating a number of aged tissues, such as heart, skeletal muscle and brain. However, recent reports have shown contradictory data questioning its capacity to reverse age-related tissue dysfunction. The availability of a mouse model of accelerated aging, which shares most of the features occurring in physiological aging, gives us an excellent opportunity to test in vivo therapies aimed at extending lifespan both in pathological and normal aging. On this basis, we wondered whether the proposed anti-aging functions of GDF11 would have an overall effect on longevity. We first confirmed the existence of a reduction in GDF11/8 levels in our mouse model of accelerated aging compared with wild-type littermates. However, we show herein that GDF11 daily administration does not extend lifespan of premature-aged mice. PMID:27507054

  1. Green tea and the skin.

    Science.gov (United States)

    Hsu, Stephen

    2005-06-01

    Plant extracts have been widely used as topical applications for wound-healing, anti-aging, and disease treatments. Examples of these include ginkgo biloba, echinacea, ginseng, grape seed, green tea, lemon, lavender, rosemary, thuja, sarsaparilla, soy, prickly pear, sagebrush, jojoba, aloe vera, allantoin, feverwort, bloodroot, apache plume, and papaya. These plants share a common character: they all produce flavonoid compounds with phenolic structures. These phytochemicals are highly reactive with other compounds, such as reactive oxygen species and biologic macromolecules, to neutralize free radicals or initiate biological effects. A short list of phenolic phytochemicals with promising properties to benefit human health includes a group of polyphenol compounds, called catechins, found in green tea. This article summarizes the findings of studies using green tea polyphenols as chemopreventive, natural healing, and anti-aging agents for human skin, and discusses possible mechanisms of action.

  2. Protective Effects of Schisandrin A, B and C on the Oxidative Damage of HaCat Cells%五味子甲素·乙素及丙素对HaCat细胞氧化损伤的保护作用研究

    Institute of Scientific and Technical Information of China (English)

    侯微; 魏忠宝; 高薇; 王英平

    2013-01-01

    [目的]探讨五味子甲素、乙素及丙素对人皮肤细胞衰老作用的影响,为开发北五味子抗皮肤衰老产品奠定基础.[方法]通过H2O2造成HaCat细胞氧化损伤衰老模型,采用噻唑蓝(MTT)法测定HaCat细胞增殖情况.[结果]浓度50 μmol/L的H2O2对细胞无明显抑制作用,浓度100和200μmol/L组有明显抑制作用.H2O2组细胞的存活率为(71.667±0.862)%.浓度100和200 μmol/L五味子甲素组细胞的存活率分别为(76.400±0.656)%和(82.367±0.666)%;浓度100和200 μmol/L五味子乙素组细胞的存活率分别为(86.300±0.721)%和(88.900±0.985)%;浓度100和200 μmol/L五味子丙素组细胞的存活率分别为(86.767±0.551)%和(88.067±0.873)%.[结论]五味子甲素、乙素及丙素对H2O2造成细胞的氧化损伤均具有一定的保护作用,以五味子乙素和丙素的效果较好.%[Objective] To research the protective effects of schisandrin A, B and C on the oxidative damage of HaCat cells, so as to provide foundations for the development of schisandrin anti - aging products. [Method] Aging model was established based on the oxidative damage of HaCat cells induced by H2O2. And proliferation of HaCat cells was detected by MTT assay. [Result] 50 ujnol/L H2O2 showed no inhibitory effects on cells; but 100 and 200 μmol/L groups had significant inhibitory effects. The survival rate of cells in H2O2 group was (71. 667 ± 0. 862) %. The survival rates of cells in 100 and 200 μmol/L schisandrin A groups were ( 76.400 ± 0.656 ) % and ( 82.367 ± 0.666 ) , respectively. The survival rates of cells in 100 and 200 μmol/L schisandrin B groups were (86. 300 ±0.721)% and (88.900 ±0.985)% , respectively. The survival rates of cells in 100 and 200 μmol/L schisandrin C groups were (86.767 ±0.551)% and (88.067 ±0.873)% , respectively. [Conclusion] Schisandrin A, B and C all had certain protective effects on the oxidative damage of HaCat cells induced by H2O2. And achisandrin B

  3. 抗衰老中药的药理分析

    Institute of Scientific and Technical Information of China (English)

    姜一平; 李宁; 邓锡岳

    2014-01-01

    Objective Analysis on Chinese traditional medicine,understand the anti-aging ability of medicinal herbs in anti-aging effect. Methods Select 100 cases of healthy body the research object,its were randomly divided into control group and observation group,each group of 50 people. Control group subjects not TCM intervention,observation groups use intervention has anti-aging ability of traditional Chinese medicine research. Compared the two groups after two years study of plasma SP contrast differences in aspects of content,heart condition,understanding of anti-aging medicine recuperates the aging degree under study.Results Two groups before intervention in plasma,no significant difference in heart status,but two years later the use of anti-aging medicine research object plasma SP content significantly increased,and cardiac ultrasound index optimization effect significantly,compared with before (P<0.05). Anti-aging medicine,therefore,is to optimize the content of plasma SP and heart status,to optimize the body function,delay the aging degree of utility.Conclusion Researchers condition was optimized by using Chinese herbal medicine effective anti-ageing,clear the excessive free radicalsin the human body,improve body immunity and the body running environment,part of Chinese traditional medicine can rise to the activation cell function,so under the pharmacological analysis proved that the research of traditional Chinese medicine have anti-aging ability.%目的:针对我国中药药材展开分析,了解具有抗衰老能力的药材在延缓衰老方面的效果。方法选取珠海市人民医院100例身体健康研究对象,将其随机均分为对照组和观察组(n=50)。对照组研究对象不进行中药干预,观察组研究对象使用具有抗衰老能力的中药材进行干预研究。在2年时间后对比2组研究对象血浆SP含量、心脏状况等方面的对比差异性,了解抗衰老中药调理下研究对象衰老程度。结果2

  4. A novel, volumizing cosmetic formulation significantly improves the appearance of target Glabellar lines, nasolabial folds, and crow's feet in a double-blind, vehicle-controlled clinical trial.

    Science.gov (United States)

    Farris, Patricia K; Edison, Brenda L; Weinkauf, Ronni L; Green, Barbara A

    2014-01-01

    Facial lines and wrinkles are caused by many factors including constant exposure to external elements, such as UV rays, as well as the dynamic nature of facial expression. Many cosmetic products and procedures provide global improvement to aging skin, whereas injectable therapies are frequently utilized to diminish specific, target wrinkles. Despite their broad availability, some patients are unwilling to undergo injectables and would benefit from an effective topical option. A noninvasive option to volumize target wrinkle areas could also extend benefits of commonly used cosmetic anti-aging products. To this end, a two-step formulation containing the novel, cosmetic anti-aging ingredient, N-acetyl tyrosinamide, was developed for use on targeted wrinkle areas. The tolerability and efficacy of the serum plus cream were tested for 16 weeks in women with moderate facial photodamage on predetermined wrinkle areas (glabellar lines, nasolabial folds, under eye lines, and lateral canthal (crow's feet) wrinkles) in a single-center, randomized, double-blind, vehicle-controlled, clinical trial. Seventy women (47 Active group, 23 Vehicle group) completed the study. Digital photography, clinical grading, ultrasound and self-assessment scores confirmed improvement to wrinkle areas. The topical cosmetic formulation was statistically superior (Pcosmetic formulation reduced the appearance of wrinkles and increased skin elasticity thus providing an effective anti-aging option for target wrinkle areas. This study suggests that in addition to its use as monotherapy for reducing targeted lines and wrinkles this cosmetic formulation may be also serve as an adjuvant to injectable therapies.

  5. TREATMENT EFFECTS 101

    Directory of Open Access Journals (Sweden)

    Thelma J. Mielenz

    2015-10-01

    Full Text Available Physical therapy researchers are interested in how beneficial an intervention is or the “treatment effect.” There are many measures of treatment effect that are applicable for understanding the efficacy and effectiveness of health interventions. Given that each treatment effect has its own set of advantages and disadvantages, understanding these characteristics can help guide which measure is most appropriate for a specific study. This article presents the more common treatment effects for both dichotomous and continues outcomes. The overall aim is to serve as a guide to newer physical therapy researchers on using and interpreting treatment effects.

  6. Experiences with effects specifications

    DEFF Research Database (Denmark)

    Simonsen, Jesper; Hertzum, Morten; Barlach, Anders

    2011-01-01

    We describe the effects-specification process from a project that was conducted during the fall 2010 and spring of 2011 in this chapter. The project configured and implemented an electronic patient record system at a maternity ward at a hospital located in a European region. The process comprised...... workshops with effects specification with management and end-users and an agile development process including prototypes configured from the effects specifications. We describe the project and the effects-specification process through which effects were related to the system design and instruments...... for measuring effects were designed. The project is analyzed and lessons learned are discussed....

  7. [Placebo and placebo effect].

    Science.gov (United States)

    Aulas, J-J

    2005-11-01

    The word placebo appeared for the first time in an English medical dictionary in 1785. In French, it appeared much latter in 1958. This word defines an experimental tool used for rigourous evaluation of a specific effect of pharmacological treatment and the non specific effect of any therapy. The placebo effect is the strictly psychological or psychophysiological effect of a placebo. The two principal components of placebo effect as a pain killer, which has been extensively studied in this field, are positive expectancies of both the patient and the physician. Although the mechanisms of action of placebo effect are not well understood, results of several recent works are particularly interesting.

  8. Effective Business Communication requires effective practices

    Institute of Scientific and Technical Information of China (English)

    杨福明

    2011-01-01

    @@ The topic of this essay is that effective businees communication requires effective practices.This essay will focus on communication practices that can assist an organi- zation in reaching its strategic goals and objectives.This article will present this topic by analyzing the communication theory, writing process and business writing style, team building and interpersonal communication, negotiation and persuasion tech- niques and intercultural communication.

  9. THE ANTIOXIDATIVE EFFECT OF α-LIPOIC ACID ON BLOOD CELL MITOCHONDRIA OF AGED RATS%α-硫辛酸对老年大鼠外周血细胞线粒体的抗氧化作用

    Institute of Scientific and Technical Information of China (English)

    田芳; 赵鹏; 袁准; 孙宇立; 邵春海; 刘景芳

    2012-01-01

    week and maintained in the subsequent stages. Conclusion LA scavenges ROS and protects mitochondria from oxidative damages, thus plays an important role in antioxidantive and anti-aging activities, which depend on its concentration and duration in vivo.

  10. Effects of heat treatment and storage temperature on storage qualities of strawberry%热处理与贮藏温度对草莓果实保鲜效果的影响

    Institute of Scientific and Technical Information of China (English)

    聂凌鸿; 吴林林

    2012-01-01

    This thesis is based on the experimental material shed fresh strawberries were of two kinds of heat treatment (40 % hot air, 15 rain and 40 ℃ sterile water, 15 min) and different storage temperatures (room temperature 20 ℃ and low temperature 4 %) on strawberry decay rate, rate of weight loss. The results showed that heat treatment was conducive to the preservation of strawberries, in which hot air treatment is better than hot water, cold storage better than the normal temperature. Based on this conclusion, fresh strawberries for the basis of the same experimental materials to study three different temperatures (35, 40, 45 %) of hot air and at different times (10, 15, 20 min) treatments on strawberry color, smell, hardness, acidity, sugar content and respiration. The results showed that heat treatment could significantly affect the physiology of strawberry and quality of storage, heat treatment could be maintained during storage of strawberry color, odor, hardness, reducing their respiration rate, reduce the sugar and acid in strawberry fruit loss, anti aging strawberries. Among them, the effect of 10 min hot air at 45 ℃ was the best.%以大棚新鲜草莓为实验材料,分别研究2种热处理方式(40℃热空气,15min和40℃无菌水,15rain)以及不同贮藏温度(常温20℃和低温4℃)对草莓的腐烂率、失重率的影响。结果表明,热处理明显有利于草莓的保鲜,其中热空气处理优于热水处理,低温贮藏优于常温贮藏。基于这一结论后,又以相同的新鲜草莓为实验材料,研究3种不同温度(35、40、45℃)的热空气和不同时间(10、15、20min)处理对草莓色泽、气味、硬度、酸度、糖含量以及呼吸强度的影响。结果表明,热处理能明显影响草莓贮藏期的生理和品质,热处理可保持草莓贮藏过程中的色泽、气味、硬度,降低其呼吸强度,减少草莓果实中糖和酸的损失,

  11. Effects of rapamycin induced cellular autophagy in aging-related diseases%雷帕霉素诱导细胞自噬在衰老相关疾病中的作用

    Institute of Scientific and Technical Information of China (English)

    吴伯艳; 刘新光; 陈维春

    2015-01-01

    哺乳动物雷帕霉素靶蛋白( mammalian target of rapamy-cin, mTOR)是衰老和衰老相关疾病的一个关键调节因子。雷帕霉素( rapamycin, RAPA)可通过抑制mTOR通路,诱导和促进细胞自噬的发生。细胞自噬是维持细胞内稳态的主要方式与途径,通过降解多余的、受损的及衰老的蛋白与细胞器,为细胞重建、再生和修复提供必需原料。早老症( hutchinson-gil-ford progeria syndrome, HGPS )患者细胞中伴随早老蛋白( progerin)的异常聚集;此外,诸如亨廷顿病、帕金森病、阿尔茨海默病等神经退行性疾病细胞内同样出现异常蛋白质的聚集,而这些异常蛋白的清除正依赖于细胞的自噬作用。由此可见,雷帕霉素是潜在的抗衰老、治疗早老症及衰老相关疾病的重要药物。该文主要阐述雷帕霉素促进细胞自噬方面的功能及在HGPS、神经退行性疾病方面的应用。%Mammalian target of rapamycin( mTOR) is a key reg-ulator of aging and aging-related diseases. Rapamycin ( RAPA) induces and promotes the process of cell autophagy through in-hibiting mTOR pathway. Autophagy exerts a crucial role in main-taining the cellular meostasis, which provides essential materials for cell reconstruction, regeneration and repair via degradating the redundant, damaged, or senescent proteins and organelles. Hutchinson Gilford progeria syndrome ( HGPS ) patients are al-ways accompanied with abnormally accumulated progerin in cells. Similar to HGPS, abnormal protein accumulation is the common pathological feature of neurodegenerative diseases, in-cluding Huntington′s disease, Parkinson′s disease, Alzheimer′s disease and so on. Degradation of these abnormal proteins pre-dominantly depends on cell autophagy. Thus, rapamycin is a po-tential anti-aging drug for HGPS and aging-related diseases thera-py. This view focuses on the effects of rapamycin on cell autoph-agy and clinical application in HGPS and neurodegenerative

  12. Gravitomagnetic Effect in Magnetars

    CERN Document Server

    Chatterjee, Debarati; Bandyopadhyay, Debades

    2016-01-01

    Rotating bodies in General Relativity produce frame dragging (or Lense-Thirring effect), also known as the {\\it Gravitomagnetic effect} in analogy with Classical Electromagnetism. In this work, we study the effect of strong magnetic fields in neutron stars on the Gravitomagnetic effect, which is produced as a result of its rotation. We show that the magnetic field has a non-negligible impact on frame dragging. The maximum effect of the magnetic field appears along the polar direction, where the Lense-Thirring frequency decreases with increase in magnetic field, and along the equatorial direction, where its magnitude increases. For intermediate angles, the effect of the magnetic field decreases, and goes through a minimum for a particular angular value at which magnetic field has no effect on Gravitomagnetism. Beyond that particular angle Gravitomagnetic effect increases with increasing magnetic field. We try to identify this "Null Region" for the case of magnetars, both inside and outside, as a function of th...

  13. Side Effects (Management)

    Science.gov (United States)

    ... cancer care is relieving side effects, called symptom management, palliative care, or supportive care. It is important ... treat them. To learn about the symptoms and management of the long-term side effects of cancer ...

  14. Memory effects in turbulence

    Science.gov (United States)

    Hinze, J. O.

    1979-01-01

    Experimental investigations of the wake flow of a hemisphere and cylinder show that such memory effects can be substantial and have a significant influence on momentum transport. Memory effects are described in terms of suitable memory functions.

  15. Prooxidant effects of nitrofurantoin.

    Science.gov (United States)

    Novikov, O O; Pokrovskii, M V; Konovalenko, A B

    2002-08-01

    We studied the possibility of using prooxidant effects of nitrofurantoin (furadonin) for stimulation of the natural antioxidant systems for preventing myocardial damage after coronary occlusion. A pronounced cardioprotective effect of the drug was observed.

  16. Characteristics of Effective Argumentation.

    Science.gov (United States)

    Frana, Adrian W.

    1989-01-01

    Examines how the 1988 Presidential Debates provide a resource for effective instruction in public argument. Provides several examples of effective (and ineffective) argumentative speaking taken from the debates. (MM)

  17. The Hydrophobic Effect.

    Science.gov (United States)

    Huque, Entazul M.

    1989-01-01

    Discusses the physical basis and current understanding of hydrophobic effects. The thermodynamic background of the effects, hydrophobic hydration, and hydrophobic interactions are described. Four existing controversies are outlined. (YP)

  18. Effects of teacher training

    DEFF Research Database (Denmark)

    Wahlgren, Bjarne; Larsen, Lea Lund

    2010-01-01

    The article gives a short overview over existing knowledge concerning the effect of teacher training in relation to adult learning. It presents a research design for measuring the effect of teacher traning.......The article gives a short overview over existing knowledge concerning the effect of teacher training in relation to adult learning. It presents a research design for measuring the effect of teacher traning....

  19. Photon thermal Hall effect

    CERN Document Server

    Ben-Abdallah, Philippe

    2015-01-01

    A near-field thermal Hall effect (i.e.Righi-Leduc effect) in lattices of magneto-optical particles placed in a constant magnetic field is predicted. This effect is related to a symetry breaking in the system induced by the magnetic field which gives rise to preferential channels for the heat-transport by photon tunneling thanks to the particles anisotropy tuning.

  20. Magnetic effects in electrochemistry

    Directory of Open Access Journals (Sweden)

    NEBOJSA D. NIKOLIC

    2005-05-01

    Full Text Available The effect of imposed magnetic fields onto the electrodeposition of magnetic (nickel and non – magnetic (copper metals was analysed. Also, magnetic properties of electrochemically obtained nanocontacts were examined. An effort to establish a possible correlation between the morphologies of the nanocontacts and the effect of the very large ballistic magnetoresistance (BMR effect was made.

  1. Effective graph resistance

    NARCIS (Netherlands)

    Ellens, W.; Spieksma, F.M.; Mieghem, P. van; Jamakovic, A.; Kooij, R.E.

    2011-01-01

    This paper studies an interesting graph measure that we call the effective graph resistance. The notion of effective graph resistance is derived from the field of electric circuit analysis where it is defined as the accumulated effective resistance between all pairs of vertices. The objective of the

  2. Binary effectivity rules

    DEFF Research Database (Denmark)

    Keiding, Hans; Peleg, Bezalel

    2006-01-01

    is binary if it is rationalized by an acyclic binary relation. The foregoing result motivates our definition of a binary effectivity rule as the effectivity rule of some binary SCR. A binary SCR is regular if it satisfies unanimity, monotonicity, and independence of infeasible alternatives. A binary...... effectivity rule is regular if it is the effectivity rule of some regular binary SCR. We characterize completely the family of regular binary effectivity rules. Quite surprisingly, intrinsically defined von Neumann-Morgenstern solutions play an important role in this characterization...

  3. Enhanced magnetocaloric effect material

    Science.gov (United States)

    Lewis, Laura J. H.

    2006-07-18

    A magnetocaloric effect heterostructure having a core layer of a magnetostructural material with a giant magnetocaloric effect having a magnetic transition temperature equal to or greater than 150 K, and a constricting material layer coated on at least one surface of the magnetocaloric material core layer. The constricting material layer may enhance the magnetocaloric effect by restriction of volume changes of the core layer during application of a magnetic field to the heterostructure. A magnetocaloric effect heterostructure powder comprising a plurality of core particles of a magnetostructural material with a giant magnetocaloric effect having a magnetic transition temperature equal to or greater than 150 K, wherein each of the core particles is encapsulated within a coating of a constricting material is also disclosed. A method for enhancing the magnetocaloric effect within a giant magnetocaloric material including the step of coating a surface of the magnetocaloric material with a constricting material is disclosed.

  4. EFFECTIVE DISCHARGE CALCULATION GUIDE

    Institute of Scientific and Technical Information of China (English)

    D.S.BIEDENHARN; C.R.THORNE; P.J.SOAR; R.D.HEY; C.C.WATSON

    2001-01-01

    This paper presents a procedure for calculating the effective discharge for rivers with alluvial channels.An alluvial river adjusts the bankfull shape and dimensions of its channel to the wide range of flows that mobilize the boundary sediments. It has been shown that time-averaged river morphology is adjusted to the flow that, over a prolonged period, transports most sediment. This is termed the effective discharge.The effective discharge may be calculated provided that the necessary data are available or can be synthesized. The procedure for effective discharge calculation presented here is designed to have general applicability, have the capability to be applied consistently, and represent the effects of physical processes responsible for determining the channel, dimensions. An example of the calculations necessary and applications of the effective discharge concept are presented.

  5. Effective School Counseling Teams

    OpenAIRE

    Lilley, Stacey Custer

    2007-01-01

    Despite much attention given to effective teams in the workplace, school counseling teams have been neglected in the research. The primary purpose of this mixed methods study was to learn what characteristics secondary counselors perceive contribute to an effective school counseling team. The first research phase conducted six team interviews; themes emerging from the interviews yielded the development of the Effective School Counseling Team Questionnaire (ESCTQ). The following research quest...

  6. The Hubble effective potential

    Energy Technology Data Exchange (ETDEWEB)

    Janssen, T.M.; Miao, S.P.; Prokopec, T. [Institute for Theoretical Physics and Spinoza Institute, Utrecht University, Leuvenlaan 4, Postbus 80.195, 3508 TD Utrecht (Netherlands); Woodard, R.P., E-mail: T.M.Janssen@uu.nl, E-mail: S.Miao@uu.nl, E-mail: T.Prokopec@uu.nl, E-mail: woodard@phys.ufl.edu [Department of Physics, University of Florida, Gainesville, FL 32611 (United States)

    2009-05-15

    We generalize the effective potential to scalar field configurations which are proportional to the Hubble parameter of a homogeneous and isotropic background geometry. This may be useful in situations for which curvature effects are significant. We evaluate the one loop contribution to the Hubble Effective Potential for a massless scalar with arbitrary conformal and quartic couplings, on a background for which the deceleration parameter is constant. Among other things, we find that inflationary particle production leads to symmetry restoration at late times.

  7. Atomic lighthouse effect.

    Science.gov (United States)

    Máximo, C E; Kaiser, R; Courteille, Ph W; Bachelard, R

    2014-11-01

    We investigate the deflection of light by a cold atomic cloud when the light-matter interaction is locally tuned via the Zeeman effect using magnetic field gradients. This "lighthouse" effect is strongest in the single-scattering regime, where deviation of the incident field is largest. For optically dense samples, the deviation is reduced by collective effects, as the increase in linewidth leads to a decrease in magnetic field efficiency.

  8. The Atomic Lighthouse Effect

    CERN Document Server

    Máximo, C E; Courteille, Ph W; Bachelard, R

    2014-01-01

    We investigate the deflection of light by a cold atomic cloud when the light-matter interaction is locally tuned via the Zeeman effect using magnetic field gradients. This "lighthouse" effect is strongest in the single-scattering regime, where deviation of the incident field is largest. For optically dense samples, the deviation is reduced by collective effects, as the increase in linewidth leads to a decrease of the magnetic field efficiency.

  9. Impedance and Collective Effects

    CERN Document Server

    Metral, E; Rumolo, R; Herr, W

    2013-01-01

    This document is part of Subvolume C 'Accelerators and Colliders' of Volume 21 'Elementary Particles' of Landolt-Börnstein - Group I 'Elementary Particles, Nuclei and Atoms'. It contains the Chapter '4 Impedance and Collective Effects' with the content: 4 Impedance and Collective Effects Introduction 4.1 Space Charge 4.2 Wake Fields and Impedances 4.3 Coherent Instabilities 4.4 Landau Damping 4.5 Two-Stream Effects (Electron Cloud and Ions) 4.6 Beam-Beam Effects 4.7 Numerical Modelling

  10. On the butterfly effect

    CERN Document Server

    Shnirelman, Alexander

    2016-01-01

    The term "butterfly effect" means an extreme sensitivity of a dynamical system to small perturbations: "The beating of a butterfly wing in South America can result in the considerable change of positions and force of a tropical cyclon in Atlantic 2 weeks later". Numerical simulations of R.Robert show the absence of the butterfly effect in some simple flows of 2-d ideal incompressible fluid which is a model of the atmosphere. In this work a more complicated flow is considered. Numerical simulation demonstrates the butterfly effect in the strongest form. The effect is robust, and the experiment is 100% reproducible.

  11. Effect of alcohol extract of Cortex Eucommiae on oxidative stress markers in serum of brain aging model mice%杜仲乙醇提取物对脑老化模型小鼠血中氧化应激指标的影响

    Institute of Scientific and Technical Information of China (English)

    马山; 孙金杰; 王欣; 李淼; 杜丽娜; 田景振

    2016-01-01

    目的:探讨杜仲对脑老化模型小鼠血中氧化应激指标的影响。方法皮下注射D-半乳糖建立小鼠老龄化模型,杜仲乙醇提取物灌胃给药。采用流式细胞仪测定不同组小鼠脑细胞凋亡率,采用ELISA试剂盒测定一系列氧化指标,包括丙二醛(MDA)、超氧化物歧化酶(SOD)、蛋白质羰基含量(PCO)和一氧化氮(NO)含量。结果流式细胞仪测定结果显示,与模型组相比,杜仲提取物组小鼠脑细胞凋亡率明显下降(P<0.01)。ELISA测定结果表明,与正常组相比,杜仲组小鼠血中NO和SOD水平显著升高(P<0.01)。与模型组相比,杜仲组小鼠血中MDA和PCO水平显著降低(P<0.01),而SOD水平显著升高(P<0.01)。结论杜仲提取物可能通过有效减轻氧化损伤而发挥其抗脑老化作用。%Objective To explore the effect of Cortex Eucommiae(CE)extract on oxidative stress markers of brain aging mice. Methods Brain aging mice model was made via subcutaneously administered D-galactose. The ethanol extract of CE was intra⁃gastrically administered to the model mice. Apoptosis ratio of brain cells of mice was determined by a flow cytometry to evaluate the an⁃ti-aging function of CE extract. A series of biomarkers related to oxidation,including malonyldialdehyde(MDA),superoxide dis⁃mutase(SOD),protein carbonyl(PCO),and nitric oxide(NO)in serum of mice were determined by ELISA. Results Compared with the model mice,the apoptosis ratio of brain cells in the CE extract group decreased significantly(P<0.01). ELISA test results showed that,compared with the normal group,NO and SOD levels in serum of CE extract group were significantly higher(P<0.01). Compared with the model group,MDA and PCO levels in serum of CE extract group were significantly lower(P<0.01),and SOD level was obviously higher(P<0.01). Conclusion The CE extract might play the role of brain anti-aging by the effective attenuation of

  12. School effectiveness research

    NARCIS (Netherlands)

    Scheerens, J.; Wright, James D.

    2015-01-01

    School effectiveness research is described as the scientific approach to determine the causal influence of malleable conditions of schooling. The article describes how different strands of school effectiveness have developed and are now increasingly combined into more integrative approaches. The kno

  13. School Effectiveness and Leadership.

    Science.gov (United States)

    Dow, I. I.; Oakley, W. F.

    1992-01-01

    Fiedler's contingency theory relates school effectiveness to a combination of principals' leadership style and situational favorability for the principal. Data from teacher questionnaires on school climate and effectiveness and measures of principal's leadership in 176 Canadian elementary schools did not support Fiedler's model. Contains 54…

  14. Nonlocal Anomalous Hall Effect.

    Science.gov (United States)

    Zhang, Steven S-L; Vignale, Giovanni

    2016-04-01

    The anomalous Hall (AH) effect is deemed to be a unique transport property of ferromagnetic metals, caused by the concerted action of spin polarization and spin-orbit coupling. Nevertheless, recent experiments have shown that the effect also occurs in a nonmagnetic metal (Pt) in contact with a magnetic insulator [yttrium iron garnet (YIG)], even when precautions are taken to ensure that there is no induced magnetization in the metal. We propose a theory of this effect based on the combined action of spin-dependent scattering from the magnetic interface and the spin-Hall effect in the bulk of the metal. At variance with previous theories, we predict the effect to be of first order in the spin-orbit coupling, just as the conventional anomalous Hall effect-the only difference being the spatial separation of the spin-orbit interaction and the magnetization. For this reason we name this effect the nonlocal anomalous Hall effect and predict that its sign will be determined by the sign of the spin-Hall angle in the metal. The AH conductivity that we calculate from our theory is in order of magnitude agreement with the measured values in Pt/YIG structures.

  15. The Creativity Passdown Effect

    DEFF Research Database (Denmark)

    Pries-Heje, Jan; Lee, Jong Seok; Baskerville, Richard

    2015-01-01

    effect in which the creative thinking of a team of design theorist(s) inherent in DT invokes a creative mind of a team of artifact instance designer(s) in creating an IDA. In this study, the authors empirically investigate the creativity passdown effect through an action case in which a DT (DT nexus...

  16. Presenting Food Science Effectively

    Science.gov (United States)

    Winter, Carl K.

    2016-01-01

    While the need to present food science information effectively is viewed as a critical competency for food scientists by the Institute of Food Technologists, most food scientists may not receive adequate training in this area. Effective presentations combine both scientific content and delivery mechanisms that demonstrate presenter enthusiasm for…

  17. The Chelate Effect Redefined.

    Science.gov (United States)

    da Silva, J. J. R. Frausto

    1983-01-01

    Discusses ambiguities of the accepted definition of the chelate effect, suggesting that it be defined in terms of experimental observation rather than mathematical abstraction. Indicates that the effect depends on free energy change in reaction, ligand basicity, pH of medium, type of chelates formed, and concentration of ligands in solution. (JN)

  18. The Aid Effectiveness Literature

    DEFF Research Database (Denmark)

    Doucouliagos, Hristos; Paldam, Martin

    The AEL consists of empirical macro studies of the effects of development aid. At the end of 2004 it had reached 97 studies of three families, which we have summarized in one study each using meta-analysis. Studies of the effect on investments show that they rise by 1/3 of the aid – the rest is c...

  19. Occlusion effects, Part II

    DEFF Research Database (Denmark)

    Hansen, Mie Østergaard

    The present report studies the mechanism of the occlusion effect by means of literature studies, experiments and model estimates. A mathematical model of the occlusion effect is developed. The model includes the mechanical properties of the earmould and the airborne sound as well as the body...

  20. Network effects in railways

    DEFF Research Database (Denmark)

    Landex, Alex

    2012-01-01

    Railway operation is often affected by network effects as a change in one part of the network can influence other parts of the network. Network effects occur because the train runs may be quite long and since the railway system has a high degree of interdependencies as trains cannot cross...

  1. Relative Effects at Work

    NARCIS (Netherlands)

    Braeken, Johan; Mulder, Joris; Wood, Stephen

    2015-01-01

    Assessing the relative importance of predictors has been of historical importance in a variety of disciplines including management, medicine, economics, and psychology. When approaching hypotheses on the relative ordering of the magnitude of predicted effects (e.g., the effects of discrimination

  2. Study of Alkylglycerol Containing Shark Liver Oil: a Physico Chemical Support for Biological Effect?

    Directory of Open Access Journals (Sweden)

    Jean-Claude Debouzy

    2008-05-01

    Full Text Available Shark liver oil (SLO, is used in natural medicine as immunity stimulant, cardiovascular protector and anti ageing reagent. These properties were related with the high amounts of alkylglycerols (22% obtained from Greenland shark liver. After a control of the mean SLO composition by NMR and MS, surface and membrane interactions and antioxidant properties were investigated using NMR, ESR and ST measurements and the in vitro consequences on erythrocytes and cells were studied. An estimation of the composition of this extract was performed. Moreover, SLO was found not haemolytic (A concentration inducing 50% haemolysis, HC50 could not be reached and superficial tension measurements revealed slight tension active properties. The 31P and 2H –NMR and ESR studies of phospholipid dispersions (dimyristoyl phosphatidyl cholin, DMPC in the presence of SLO showed a significant increase in membrane fluidity at low temperature (below phase transition temperature predominantly observed at the surface level. The anti oxidant activity was also confirmed, similar as that observed for vitamin E.

  3. Nocebo effect in dermatology

    Directory of Open Access Journals (Sweden)

    Sidharth Sonthalia

    2015-01-01

    Full Text Available Nocebo effect, originally denoting the negative counterpart of the placebo phenomenon, is now better defined as the occurrence of adverse effects to a therapeutic intervention because the patient expects them to develop. More commonly encountered in patients with a past negative experience, this effect stems from highly active processes in the central nervous system, mediated by specific neurotransmitters and modulated by psychological mechanisms such as expectation and conditioning. The magnitude of nocebo effect in clinical medicine is being increasingly appreciated and its relevance encompasses clinical trials as well as clinical practice. Although there is hardly any reference to the term nocebo in dermatology articles, the phenomenon is encountered routinely by dermatologists. Dermatology patients are more susceptible to nocebo responses owing to the psychological concern from visibility of skin lesions and the chronicity, unpredictable course, lack of ′permanent cure′ and frequent relapses of skin disorders. While finasteride remains the prototypical drug that displays a prominent nocebo effect in dermatologic therapeutics, other drugs such as isotretinoin are also likely inducers. This peculiar phenomenon has recently been appreciated in the modulation of itch perception and in controlled drug provocation tests in patients with a history of adverse drug reactions. Considering the conflict between patients′ right to information about treatment related adverse effects and the likelihood of nocebo effect stemming from information disclosure, the prospect of ethically minimizing nocebo effect remains daunting. In this article, we review the concept of nocebo effect, its postulated mechanism, relevance in clinical dermatology and techniques to prevent it from becoming a barrier to effective patient management.

  4. [Psychoanalysis and Side Effect].

    Science.gov (United States)

    Shirahase, Joichiro

    2015-01-01

    A study of psychoanalysis from the perspective of side effects reveals that its history was a succession of measures to deal with its own side effects. This, however, does not merely suggest that, as a treatment method, psychoanalysis is incomplete and weak: rather, its history is a record of the growth and development of psychoanalysis that discovered therapeutic significance from phenomena that were initially regarded as side effects, made use of these discoveries, and elaborated them as a treatment method. The approach of research seen during the course of these developments is linked to the basic therapeutic approach of psychoanalysis. A therapist therefore does not draw conclusions about a patient's words and behaviors from a single aspect, but continues to make efforts to actively discover a variety of meanings and values from them, and to make the patient's life richer and more productive. This therapeutic approach is undoubtedly one of the unique aspects of psychoanalysis. I discuss the issue of psychoanalysis and side effects with the aim of clarifying this unique characteristic of psychoanalysis. The phenomenon called resistance inevitably emerges during the process of psychoanalytic treatment. Resistance can not only obstruct the progress of therapy; it also carries the risk of causing a variety of disadvantages to the patient. It can therefore be seen as an adverse effect. However, if we re-examine this phenomenon from the perspective of transference, we find that resistance is in fact a crucial tool in psychoanalysis, and included in its main effect, rather than a side effect. From the perspective of minimizing the character of resistance as a side effect and maximizing its character as a main effect, I have reviewed logical organization, dynamic evaluation, the structuring of treatment, the therapist's attitudes, and the training of therapists. I conclude by stating that psychoanalysis has aspects that do not match the perspective known as a side

  5. Nonlocal Anomalous Hall Effect

    Science.gov (United States)

    Zhang, Steven S.-L.; Vignale, Giovanni

    2016-04-01

    The anomalous Hall (AH) effect is deemed to be a unique transport property of ferromagnetic metals, caused by the concerted action of spin polarization and spin-orbit coupling. Nevertheless, recent experiments have shown that the effect also occurs in a nonmagnetic metal (Pt) in contact with a magnetic insulator [yttrium iron garnet (YIG)], even when precautions are taken to ensure that there is no induced magnetization in the metal. We propose a theory of this effect based on the combined action of spin-dependent scattering from the magnetic interface and the spin-Hall effect in the bulk of the metal. At variance with previous theories, we predict the effect to be of first order in the spin-orbit coupling, just as the conventional anomalous Hall effect—the only difference being the spatial separation of the spin-orbit interaction and the magnetization. For this reason we name this effect the nonlocal anomalous Hall effect and predict that its sign will be determined by the sign of the spin-Hall angle in the metal. The AH conductivity that we calculate from our theory is in order of magnitude agreement with the measured values in Pt /YIG structures.

  6. Cohomology of Effect Algebras

    Directory of Open Access Journals (Sweden)

    Frank Roumen

    2017-01-01

    Full Text Available We will define two ways to assign cohomology groups to effect algebras, which occur in the algebraic study of quantum logic. The first way is based on Connes' cyclic cohomology. The resulting cohomology groups are related to the state space of the effect algebra, and can be computed using variations on the Kunneth and Mayer-Vietoris sequences. The second way involves a chain complex of ordered abelian groups, and gives rise to a cohomological characterization of state extensions on effect algebras. This has applications to no-go theorems in quantum foundations, such as Bell's theorem.

  7. Creating more effective graphs

    CERN Document Server

    Robbins, Naomi B

    2012-01-01

    A succinct and highly readable guide to creating effective graphs The right graph can be a powerful tool for communicating information, improving a presentation, or conveying your point in print. If your professional endeavors call for you to present data graphically, here's a book that can help you do it more effectively. Creating More Effective Graphs gives you the basic knowledge and techniques required to choose and create appropriate graphs for a broad range of applications. Using real-world examples everyone can relate to, the author draws on her years of experience in gr

  8. Seebeck effect in electrolytes.

    Science.gov (United States)

    Chikina, I; Shikin, V; Varlamov, A A

    2012-07-01

    We study Seebeck effect in liquid electrolytes, starting from its simple neutral analog--thermodiffusion (so-called Ludwig-Soret or Soret effect). It is observed that when two or more subsystems of mobile particles are subjected to the temperature gradient, various types of them respond to it differently. In the case when these fractions, with different mobility parameters (Soret coefficients), are oppositely charged (a case typical for electrolytes), the nonhomogeneous internal electric field is generated. The latter field prevents these fractions from space separation and determines the intensity of the appearing Seebeck effect.

  9. Complex Effective Action and Schwinger Effect

    CERN Document Server

    Kim, Sang Pyo

    2016-01-01

    Spontaneous pair production from background fields or spacetimes is one of the most prominent phenomena predicted by quantum field theory. The Schwinger mechanism of production of charged pairs by a strong electric field and the Hawking radiation of all species of particles from a black hole are the consequence of nonperturbative quantum effects. In this review article, the vacuum structure and pair production is reviewed in the in-out formalism, which provides a consistent framework for quantum field theory in the sense that the complex action explains not only the vacuum persistence but also pair production. The current technology of intense lasers is still lower by a few order than the Schwinger limit for electron-positron pair production, while magnetic fields of magnetars on the surface are higher than the Schwinger limit and even higher at the core. On the other hand, the zero effective mass of electron and hole in graphene and Dirac or Weyl semimetals will open a window for experimental test of quantum...

  10. Vaccine herd effect.

    Science.gov (United States)

    Kim, Tae Hyong; Johnstone, Jennie; Loeb, Mark

    2011-09-01

    Vaccination ideally protects susceptible populations at high risk for complications of the infection. However, vaccines for these subgroups do not always provide sufficient effectiveness. The herd effect or herd immunity is an attractive way to extend vaccine benefits beyond the directly targeted population. It refers to the indirect protection of unvaccinated persons, whereby an increase in the prevalence of immunity by the vaccine prevents circulation of infectious agents in susceptible populations. The herd effect has had a major impact in the eradication of smallpox, has reduced transmission of pertussis, and protects against influenza and pneumococcal disease. A high uptake of vaccines is generally needed for success. In this paper we aim to provide an update review on the herd effect, focusing on the clinical benefit, by reviewing data for specific vaccines.

  11. Frost Effects Research Facility

    Data.gov (United States)

    Federal Laboratory Consortium — Full-scale study in controlled conditionsThe Frost Effects Research Facility (FERF) is the largest refrigerated warehouse in the United States that can be used for a...

  12. Stretching Safely and Effectively

    Science.gov (United States)

    ... it safely and effectively. By Mayo Clinic Staff Stretching may take a back seat to your exercise routine. The main concern is exercising, not stretching, right? Not so fast. Stretching may help you: ...

  13. Side Effects of Chemotherapy

    Science.gov (United States)

    ... Jacket Fashion Show Contact Us Side Effects of Chemotherapy Each of the chemotherapy drugs available today works in a slightly different ... few rules of thumb when it comes to chemotherapy that should always be kept in mind. Ignore ...

  14. Conditions for Effectiveness.

    Science.gov (United States)

    Wright, Jeannette T.

    1988-01-01

    The most effective college presidents are those whose leadership styles are dominant, decisive, and when appropriate, autocratic. The president has to believe profoundly in the intrinsic value of the college. (Author/MSE)

  15. The Effective Volunteer Teacher

    OpenAIRE

    2013-01-01

    This publication provides information for volunteer teachers of adults and older youth on how to conduct an effective presentation. Topics include focus of presentation, characteristics of the learners, teaching methods, visual aides and evaluations.

  16. Effects of New Technologies.

    Science.gov (United States)

    Social and Labour Bulletin, 1980

    1980-01-01

    Transnational implications of technological change and innovation in telecommunications are discussed, including impact on jobs and industrial relations, computer security, access to information, and effects of technological innovation on international economic systems. (SK)

  17. Developing Effective Working Relationships.

    Science.gov (United States)

    Bennett, Roger, Ed.; And Others

    1990-01-01

    Two workshops are described in detail. One explores ways to increase the effectiveness of work groups and includes the workshop leader's personal observations. The second involves training transformational leaders, whose characteristics include idealized influence, individualized consideration, intellectual stimulation, and inspirational…

  18. Developing Effective Performance Measures

    Science.gov (United States)

    2014-10-14

    University When Performance Measurement Goes Bad Laziness Vanity Narcissism Too Many Pettiness Inanity 52 Developing Effective...Kasunic, October 14, 2014 © 2014 Carnegie Mellon University Narcissism Measuring performance from the organization’s point of view, rather than from

  19. Frost Effects Research Facility

    Data.gov (United States)

    Federal Laboratory Consortium — Full-scale study in controlled conditions The Frost Effects Research Facility (FERF) is the largest refrigerated warehouse in the United States that can be used for...

  20. Ototoxic Medications (Medication Effects)

    Science.gov (United States)

    ... Information for the Public / Hearing and Balance Ototoxic Medications (Medication Effects) By Barbara Cone, Patricia Dorn, Dawn Konrad- ... Audiology Information Series [PDF]. What Is Ototoxicity? Certain medications can damage the ear, resulting in hearing loss, ...

  1. The Effects: Economy

    Science.gov (United States)

    Nutrient pollution has diverse and far-reaching effects on the U.S. economy, impacting tourism, property values, commercial fishing, recreational businesses and many other sectors that depend on clean water.

  2. Strategies for Effective Outsourcing.

    Science.gov (United States)

    Moneta, Larry; Dillon, William L.

    2001-01-01

    Emphasizes strategies that can be employed for effective outsourcing in higher education settings. Several models of outsourcing are identified and described, and examples of institutions using each model are provided. (GCP)

  3. Effect of xinmailong on metabolism of oxygen free radicals and content of lipofuscin in brain and hepatic tissues%心脉龙干预后氧自由基代谢及脑、肝组织脂褐质含量的变化

    Institute of Scientific and Technical Information of China (English)

    吴建新; 钮荣祥; 黄秀群; 田昆仑; 董丛丽

    2006-01-01

    于青年对照组,而心脉龙大、小剂量组均能明显降低脑组织丙二醛含量及脑组织和肝脏脂褐质含量,与衰老对照组比较差异有非常显著性意义(P<0.01).但心脉龙两剂量组差异无显著性意义.结论:心脉龙可通过改善氧自由基代谢而发挥抗衰老作用.%BACKGROUND: Chinese medicine xinmailong (XML) injection is a kind of new nucleoside compound which comes from American giant cockroach of insects. A lot of studies show that XML has obvious effect on cardiovascular activity which can treat acute or chronic congestive heart failure and shock and improve microcirculation.OBJECTIVE: To observe the effect of XML on anti-aging of mice.DESIGN: Controlled observational study.SETTING: Department of Physiology and Pathology, Basic Medical College of Dali College.MATERIALS: The experiment was carried out at the Functional Laboratory of Basic Medical College between July 2002 and September 2004. A total of 40 ICR mice of both sexes were provided by Kunming Medical College,Yunnan Naturally Occurring Drugs Key Laboratory. XML injection was provided by Yunnan Tengchong Pharmacy Factory [batch number: 94 (ZL) 03].METHODS: A total of 40 ICR mice were randomly divided into 4 groups:young control group, aging control group, XML group at high dosage and XML group at low dosage with 10 in each group. All mice were fed routinely with standard feed. Mice in young control group aged 3 months and weighed (20±2) g; meanwhile, mice in other groups aged 20 months and weighed (40±3) g. From the 20th month, mice in XML groups were intraperitoneally injected with 6 mg/kg and 8 mg/kg, respectively, for 30 days with once every day. Mice in young and aging control groups were intraperitoneally injected with the same volume of saline. On the 31st day,heads of mice were cut off to obtain blood which was maintained at hepalean anti-coagulated tube. Brains and livers were obtained rapidly for homogenate with 10% saline. Activities of catalase (CAT), superoxide

  4. Monetary Policy Proving Effective

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    @@ Hu Xiaolian,Vice Governor of the People's Bank of China,the country's central bank,published an article concerning China's managed floating exchange rate regime and the effectiveness of the monetary policy on the bank's website on July 26.She pointed out monetary policy,as an important instrument of China's macroeconomic control,has faced many challenges in recent years.A more flexible exchange rate regime will help improve the effectiveness of the policy.

  5. Radiative transfer dynamo effect

    Science.gov (United States)

    Munirov, Vadim R.; Fisch, Nathaniel J.

    2017-01-01

    Magnetic fields in rotating and radiating astrophysical plasma can be produced due to a radiative interaction between plasma layers moving relative to each other. The efficiency of current drive, and with it the associated dynamo effect, is considered in a number of limits. It is shown here, however, that predictions for these generated magnetic fields can be significantly higher when kinetic effects, previously neglected, are taken into account.

  6. Extracardiac Effects of Statins

    Directory of Open Access Journals (Sweden)

    Suleyman Ercan

    2013-01-01

    Full Text Available Statins are one of the most commonly used drugs in the world. Although it plays a valuable role in primary and secondary prevention of cardiovascular diseases, it has some extracardiac effects which are frequently ignored by physicians during routine practice. In this review we would like to summarise the potential beneficial or harmful extracardiac effects of statins which comprise a wide spectrum of systems and organs.

  7. The Cosmological Memory Effect

    OpenAIRE

    Tolish, Alexander; Wald, Robert M.

    2016-01-01

    The "memory effect" is the permanent change in the relative separation of test particles resulting from the passage of gravitational radiation. We investigate the memory effect for a general, spatially flat FLRW cosmology by considering the radiation associated with emission events involving particle-like sources. We find that if the resulting perturbation is decomposed into scalar, vector, and tensor parts, only the tensor part contributes to memory. Furthermore, the tensor contribution to m...

  8. Performance effect of Lean

    DEFF Research Database (Denmark)

    Kristensen, Thomas Borup; Israelsen, Poul

    2016-01-01

    relevant to Denmark, but the approach is empirically more generalizable. We show that the effect of Lean standardized flow production practices on performance is mediated by analytical continuous improvement empowerment practices and by delegation of decision rights practices. Thus, standardized flow...... practices do not have direct effects on performance. Instead, standardized flow provided that foundation for companies to implement continuous improvement, which, in turn, directly affect performance positively. Another cause, in addition to flow practices, of continuous improvement was the delegation...

  9. Pharmacological Effects of Mangiferin

    Institute of Scientific and Technical Information of China (English)

    WEI Zhi-quan; DENG Jia-gang; YAN Li

    2011-01-01

    Mango leaves have been widely used in the clinical practice for thousands of years in traditional Chinese medicine.Mangiferin,an effective constituent in the mango leaves,has multiple pharmacological actions involved in some basic pathological processes,such as inflammation,oxidative injury,tumor growth,micro-organism infections,metabolic regulations,and immunological regulations.The pharmacological effects of mangiferin from some published data are reviewed in this article.

  10. Advertising Effectiveness In Events

    OpenAIRE

    Jain, Sushilkumar

    2012-01-01

    Confronted with decreasing effectiveness of the classic marketing communications, events have become an increasingly popular alternative for marketers. Events constitute one of the most exciting and fastest growing forms of leisure and business. With time, the decreasing effectiveness of classical marketing communications boosted the use of events for marketing and making brand awareness. Event marketing is seen as the unique opportunity to integrate the firm’s communication activities like p...

  11. Effects of moxa and cigarette smoke on behavioral changes and brainβamyloid deposition in apoli-poprotein E-deficient mice%艾烟与香烟对载脂蛋白E基因敲除小鼠学习记忆功能与海马β淀粉样蛋白沉淀的影响

    Institute of Scientific and Technical Information of China (English)

    刘钧天; 崔莹雪; 黄玉海; 黄畅; 黄剑; 赵百孝; 韩丽; 杨佳; 王磊

    2015-01-01

    Objective To investigate the influence of moxa smoke and cigarette smoke on the apo-lipoprotein E-deficient ( ApoE-/-) male mice’ learning and memory ability andβamyloid deposition in brain hippocampus. Method 13 eight weeks old C57BL/6 mice were assigned to control group;27 eight weeks old ApoE-/- mice were randomly divided into 3 groups ( n=9/group): model group, moxa smoke group, cigarette smoke group. Mice in the two smoke groups were exposed to smoke, which concentration is con-trolled within 5~15 mg/m3;mice in model group and control group were exposed to normal air. The step-down test was conducted in the 13th week. Level ofβamyloid deposition was determined by congo red stai-ning. Results Compared with the model group, mice in control group, moxa smoke group and cigarette smoke group showed decreased learning latency, increased memory latency and made less mistakes in the step-down test (P< 0. 05). Compared with the model group,βamyloid deposition of control group, moxa smoke group and cigarette smoke group was significantly decreased ( P< 0. 05 ) . Conclusion Our find-ings suggest that moxa smoke may have effect on protecting nerve function and anti-aging by reducing the deposition of β amyloid in hippocampus.%目的:观察艾烟与香烟对载脂蛋白E基因敲除( apolipoprotein E-deficient,ApoE-/-)小鼠学习记忆能力与海马β淀粉样蛋白(β-Amyloid)沉淀的影响。方法将13只8周龄C57BL/6小鼠作为空白对照组,27只同龄ApoE-/-小鼠随机分为ApoE-/-模型组、艾烟组、香烟组。香烟与艾烟组小鼠分别暴露于5~15 mg/m3的香烟与艾烟环境。各组小鼠每天干预20分钟,每周6天,共干预12周。于第13周进行行为学测试,之后处死动物、取材,对其脑组织海马中Aβ沉淀进行刚果红染色。结果与模型组对比,空白对照组、艾烟组、香烟组小鼠均表现出学习潜伏期缩短,记忆潜伏期增长,记忆错误次数减少,差别有统计学意义( P<0.05)

  12. Habituation of reinforcer effectiveness

    Directory of Open Access Journals (Sweden)

    David R Lloyd

    2014-01-01

    Full Text Available In this paper we propose an integrative model of habituation of reinforcer effectiveness (HRE that links behavioral and neural based explanations of reinforcement. We argue that habituation of reinforcer effectiveness (HRE is a fundamental property of reinforcing stimuli. Most reinforcement models implicitly suggest that the effectiveness of a reinforcer is stable across repeated presentations. In contrast, an HRE approach predicts decreased effectiveness due to repeated presentation. We argue that repeated presentation of reinforcing stimuli decreases their effectiveness and that these decreases are described by the behavioral characteristics of habituation (McSweeney and Murphy, 2009;Rankin et al., 2009. We describe a neural model that postulates a positive association between dopamine neurotransmission and HRE. We present evidence that stimulant drugs, which artificially increase dopamine neurotransmission, disrupt (slow normally occurring HRE and also provide evidence that stimulant drugs have differential effects on operant responding maintained by reinforcers with rapid vs. slow HRE rates. We hypothesize that abnormal HRE due to genetic and/or environmental factors may underlie some behavioral disorders. For example, recent research indicates that slow-HRE is predictive of obesity. In contrast ADHD may reflect ‘accelerated-HRE’. Consideration of HRE is important for the development of effective reinforcement based treatments. Finally, we point out that most of the reinforcing stimuli that regulate daily behavior are non-consumable environmental/social reinforcers which have rapid-HRE. The almost exclusive use of consumable reinforcers with slow-HRE in pre-clinical studies with animals may have caused the importance of HRE to be overlooked. Further study of reinforcing stimuli with rapid-HRE is needed in order to understand how habituation and reinforcement interact and regulate behavior.

  13. Pleiotropic effects of statins

    Directory of Open Access Journals (Sweden)

    Narasaraju Kavalipati

    2015-01-01

    Full Text Available Statins or 3-hydroxy-methylglutaryl coenzyme A (HMG CoA reductase inhibitors not only prevents the synthesis of cholesterol biosynthesis but also inhibits the synthesis of essential isoprenoid intermediates such as farnesyl pyrophosphate, geranylgeranyl pyrophosphate, isopentanyl adenosine, dolichols and polyisoprenoid side chains of ubiquinone, heme A, and nuclear lamins. These isoprenoid intermediates are required for activation of various intracellular/signaling proteins- small guanosine triphosphate bound protein Ras and Ras-like proteins like Rho, Rab, Rac, Ral, or Rap which plays an indispensible role in multiple cellular processes. Reduction of circulating isoprenoids intermediates as a result of HMG CoA reductase inhibition by statins prevents activation of these signalling proteins. Hence, the multiple effects of statins such as antiinflammatory effects, antioxidant effects, antiproliferative and immunomodulatory effects, plaque stability, normalization of sympathetic outflow, and prevention of platelet aggregation are due to reduction of circulating isoprenoids and hence inactivation of signalling proteins. These multiple lipid-independent effects of statins termed as statin pleiotropy would potentially open floodgates for research in multiple treatment domains catching attentions of researchers and clinician across the globe.

  14. The Vampire Effect

    DEFF Research Database (Denmark)

    Erfgen, Carsten; Zenker, Sebastian; Sattler, Henrik

    2015-01-01

    Although many brand managers favor the use of celebrities in advertisements, others worry that celebrities overshadow the brand and thus impair brand recall. Practitioners refer to this overshadowing as the vampire effect, defined as a decrease in brand recall for an advertising stimulus that fea......Although many brand managers favor the use of celebrities in advertisements, others worry that celebrities overshadow the brand and thus impair brand recall. Practitioners refer to this overshadowing as the vampire effect, defined as a decrease in brand recall for an advertising stimulus...... that features a celebrity endorser versus the same stimulus with an unknown but equally attractive endorser. Because there is no agreement about whether this overshadowing really exists, this research analyzes the existence of the vampire effect and its moderators in a series of experiments with a total of 4......,970 respondents. The results provide important insights into how to avoid the vampire effect by creating appropriate conditions, such as high endorser–brand congruence or a strong cognitive link between the celebrity and the brand. Surprisingly, brand familiarity does not significantly moderate the effect....

  15. Study on Effect of Oviductus Ranae on the Expression of p16, p21 and CyclinD1 in Liver Tissues of Aged Female Rats%哈蟆油对雌性衰老大鼠肝脏组织p16,p21和细胞周期蛋白D1蛋白表达的影响

    Institute of Scientific and Technical Information of China (English)

    姚晖; 邓虹珠; 张继平; 姜晓刚; 梁颖; 黄志恩; 梁磊

    2012-01-01

    目的 探讨哈蟆油(OR)对D-半乳糖所致雌性衰老大鼠肝脏组织p16、p21和细胞周期蛋白D1蛋白表达的影响,进一步探讨哈蟆油延缓雌性大鼠机体衰老机制.方法 SPF级SD雌性青年大鼠40只随机分为D-半乳糖组、维生素E组、哈蟆油高剂量组、中剂量组、低剂量组,每组8只,D-半乳糖颈背部皮下注射42 d,建立亚急性衰老模型.另取雌性青年大鼠8只,同样部位每日注射生理盐水,作为空白对照组.第15天开始灌胃给药,给药时间28 d.给药结束后,免疫组化法检测衰老大鼠肝脏组织p16和p21蛋白的表达情况,蛋白免疫印迹法检测哈蟆油对雌性衰老大鼠肝脏组织细胞周期蛋白D1表达的影响.结果 雌性衰老大鼠肝脏组织免疫组化结果表明,p16和p21多为胞浆表达,弥漫性、灶性分布均有.D-半乳糖组p16和p21阳性细胞积分与空白组比较升高,差异有显著性(P<0.01).与D-半乳糖组相比,哈蟆油中、低剂量组,p16阳性细胞积分降低,差异有显著性(P值均<0.01),哈蟆油各剂量组p21阳性细胞积分降低,差异有显著性(P值均<0.01).肝脏组织蛋白免疫印迹法结果表明,D-半乳糖组肝脏组织细胞周期蛋白D1蛋白表达与空白组比较降低,差异有显著性(P<0.01).哈蟆油各剂量组细胞周期蛋白D1蛋白表达与D-半乳糖组比较,表达均升高(P值均<0.01),哈蟆油高剂量组尤为明显.结论 哈蟆油可降低肝脏组织p16、p21蛋白的高表达.同时显著提高细胞增殖正性调控因子细胞周期蛋白D1蛋白的表达,促进衰老雌性大鼠肝细胞增殖.哈蟆油延缓雌性衰老作用可能通过调控肝脏p16,p21,细胞周期蛋白D1信号通路来促进有关增殖调控蛋白表达发挥延缓衰老作用.%OBJECTIVE To investigate the effect of Oviductus Ranae (OR) on the expression of pl6, p21 and cyclinDl protein in liver tissue of D-galactose-induced aging female rats and to further explore the anti-aging

  16. Conditional Aid Effectiveness

    DEFF Research Database (Denmark)

    Doucouliagos, Hristos; Paldam, Martin

    The AEL (aid effectiveness literature) studies the effect of development aid using econometrics on macro data. It contains about 100 papers of which a third analyzes conditional models where aid effectiveness depends upon z, so that aid only works for a certain range of the variable. The key term...... in this family of AEL models is thus an interaction term of z times aid. The leading candidates for z are a good policy index and aid itself. In this paper, meta-analysis techniques are used (i) to determine whether the AEL has established the said interaction terms, and (ii) to identify some of the determinants...... of the differences in results between studies. Taking all available studies in consideration, we find no support for conditionality with respect to policy, while conditionality regarding aid itself is dubious. However, the results differ depending on the authors’ institutional affiliation....

  17. The Mozart Effect.

    Science.gov (United States)

    Hughes, John R.

    2001-10-01

    This review deals with the Mozart Effect, an improvement of performance while listening to Mozart music. Previous studies have shown improved spatial temporal reasoning and improved IQ test results and neurophysiological changes, mainly increased coherence among different groups of subjects. This review emphasizes the effect on epileptiform patterns, both generalized and focal; provides an example of a chronic effect over a period of 1-2 days; addresses the distinctive aspects of the music to account for this phenomenon and shows that long-term periodicity in the power of the music is a special quality; and deals with the melodic line and shows that Mozart repeats the melodic line much more frequently than other well-known composers. It is likely that the superorganization of the cerebral cortex resonates with great organization found in Mozart music.

  18. Security effectiveness review (SER)

    Energy Technology Data Exchange (ETDEWEB)

    Kouprianova, I. [Inst. of Physics and Power Engineering, Obninsk (Russian Federation); Ek, D.; Showalter, R. [Sandia National Labs., Albuquerque, NM (United States); Bergman, M. [Lawrence Livermore National Lab., CA (United States)

    1998-08-01

    As part of the on-going DOE/Russian MPC and A activities at the Institute of Physics and Power Engineering (IPPE) and in order to provide a basis for planning MPC and A enhancements, an expedient method to review the effectiveness of the MPC and A system has been adopted. These reviews involve the identification of appropriate and cost-effective enhancements of facilities at IPPE. This effort requires a process that is thorough but far less intensive than a traditional vulnerability assessment. The SER results in a quick assessment of current and needed enhancements. The process requires preparation and coordination between US and Russian analysts before, during, and after information gathering at the facilities in order that the analysis is accurate, effective, and mutually agreeable. The goal of this paper is to discuss the SER process, including the objectives, time scale, and lessons learned at IPPE.

  19. Effective Nutritional Supplement Combinations

    Science.gov (United States)

    Cooke, Matt; Cribb, Paul J.

    Few supplement combinations that are marketed to athletes are supported by scientific evidence of their effectiveness. Quite often, under the rigor of scientific investigation, the patented combination fails to provide any greater benefit than a group given the active (generic) ingredient. The focus of this chapter is supplement combinations and dosing strategies that are effective at promoting an acute physiological response that may improve/enhance exercise performance or influence chronic adaptations desired from training. In recent years, there has been a particular focus on two nutritional ergogenic aids—creatine monohydrate and protein/amino acids—in combination with specific nutrients in an effort to augment or add to their already established independent ergogenic effects. These combinations and others are discussed in this chapter.

  20. Quantum Hamlet Effect

    CERN Document Server

    Panković, Vladan

    2009-01-01

    In this work, by use of a formalism similar to formalism of the quantum Zeno effect (decrease of the decay probability of an unstable quantum system by frequent measurements) and quantum anti-Zeno effect (increase of the decay probability of an unstable quantum system by frequent measurements), we introduce so-called quantum Hamlet effect. It represents a complete destruction of the quantum predictions on the decay probability of an unstable quantum system by frequent measurement. Precisely, by means of some especial, correctly defined, frequent measurements, decay probability of an unstable quantum system can behave as a divergent series without any definite value. In this way there is quantum mechanically completely unsolvable ``Hamlet dilemma'', to decay or not to decay.

  1. Cosmological memory effect

    Science.gov (United States)

    Tolish, Alexander; Wald, Robert M.

    2016-08-01

    The "memory effect" is the permanent change in the relative separation of test particles resulting from the passage of gravitational radiation. We investigate the memory effect for a general, spatially flat Friedmann-Lemaître-Robertson-Walker (FLRW) cosmology by considering the radiation associated with emission events involving particle-like sources. We find that if the resulting perturbation is decomposed into scalar, vector, and tensor parts, only the tensor part contributes to memory. Furthermore, the tensor contribution to memory depends only on the cosmological scale factor at the source and observation events, not on the detailed expansion history of the universe. In particular, for sources at the same luminosity distance, the memory effect in a spatially flat FLRW spacetime is enhanced over the Minkowski case by a factor of (1 +z ).

  2. The Cosmological Memory Effect

    CERN Document Server

    Tolish, Alexander

    2016-01-01

    The "memory effect" is the permanent change in the relative separation of test particles resulting from the passage of gravitational radiation. We investigate the memory effect for a general, spatially flat FLRW cosmology by considering the radiation associated with emission events involving particle-like sources. We find that if the resulting perturbation is decomposed into scalar, vector, and tensor parts, only the tensor part contributes to memory. Furthermore, the tensor contribution to memory depends only on the cosmological scale factor at the source and observation events, not on the detailed expansion history of the universe. In particular, for sources at the same luminosity distance, the memory effect in a spatially flat FLRW spacetime is enhanced over the Minkowski case by a factor of $(1 + z)$.

  3. Paramagnetic Spin Seebeck Effect

    Science.gov (United States)

    Wu, Stephen M.; Pearson, John E.; Bhattacharya, Anand

    2015-05-01

    We report the observation of the longitudinal spin Seebeck effect in paramagnetic insulators. By using a microscale on-chip local heater, we generate a large thermal gradient confined to the chip surface without a large increase in the total sample temperature. Using this technique at low temperatures (<20 K ), we resolve the paramagnetic spin Seebeck effect in the insulating paramagnets Gd3Ga5O12 (gadolinium gallium garnet) and DyScO3 (DSO), using either W or Pt as the spin detector layer. By taking advantage of the strong magnetocrystalline anisotropy of DSO, we eliminate contributions from the Nernst effect in W or Pt, which produces a phenomenologically similar signal.

  4. The quantum sweeper effect

    Science.gov (United States)

    Grössing, G.; Fussy, S.; Mesa Pascasio, J.; Schwabl, H.

    2015-07-01

    We show that during stochastic beam attenuation in double slit experiments, there appear unexpected new effects for transmission factors below a ≤ 10-4, which can eventually be observed with the aid of weak measurement techniques. These are denoted as quantum sweeper effects, which are characterized by the bunching together of low counting rate particles within very narrow spatial domains. We employ a “superclassical” modeling procedure which we have previously shown to produce predictions identical with those of standard quantum theory. Thus it is demonstrated that in reaching down to ever weaker channel intensities, the nonlinear nature of the probability density currents becomes ever more important. We finally show that the resulting unexpected effects nevertheless implicitly also exist in standard quantum mechanics.

  5. Monetary Policy Proving Effective

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    Hu Xiaolian,Vice Governor of the People’s Bank of China,the country’s central bank, published an article concerning China’s managed floating exchange rate regime and the effectiveness of the monetary policy on the bank’s website on July 26.She pointed out monetary policy,as an important instrument of China’s macroeconomic control,has faced many challenges in recent years.A more flexible exchange rate regime will help improve the effectiveness of the policy.Edited excerpts follow

  6. Level Width Broaden Effect

    Institute of Scientific and Technical Information of China (English)

    ZHANG Jing-Shang

    2004-01-01

    In fitting the double-differential measurements thelevelwidth broadening effect should be taken into account properly due to Heisenberg uncertainty.Besides level width broadening effect the energy resolution in the measurements is also needed in this procedure.In general,the traditional normal Gaussian expansion is employed.However,the research indicates that to do so in this way the energy balance could not hold.For this reason,the deformed Gaussian expansion functions with exponential form for both the single energy point and continuous spectrum are introduced,with which the normalization and energy balance conditions could hold exactly in the analytical form.

  7. Magnetic Nernst effect

    Science.gov (United States)

    Brechet, Sylvain D.; Ansermet, Jean-Philippe

    2015-09-01

    The thermodynamics of irreversible processes in continuous media predicts the existence of a magnetic Nernst effect that results from a magnetic analog to the Seebeck effect in a ferromagnet and magnetophoresis occurring in a paramagnetic electrode in contact with the ferromagnet. Thus, a voltage that has DC and AC components is expected across a Pt electrode as a response to the inhomogeneous magnetic induction field generated by magnetostatic waves of an adjacent YIG slab subject to a temperature gradient. The voltage frequency and dependence on the orientation of the applied magnetic induction field are quite distinct from that of spin pumping.

  8. Erythropoietin and Nonhematopoietic Effects.

    Science.gov (United States)

    Nekoui, Alireza; Blaise, Gilbert

    2017-01-01

    Erythropoietin (EPO) is the main regulator of red blood cell production. Since the 1990s, EPO has been used for the treatment of anemia associated with end-stage renal failure and chemotherapy. The erythropoietin receptors were found on other organs such as the brain, spinal cord, heart and skin. In addition, it has been shown that many tissues produce and locally release EPO in response to hypoxic, biochemical and physical stress. In cellular, animal and clinical studies, EPO protects tissues from ischemia and reperfusion injury, has antiapoptotic effects and improves regeneration after injury. In this article, we mainly review the nonhematopoietic effects and new possible clinical indications for EPO.

  9. Nuclear medium effects in $\

    CERN Document Server

    Haider, H; Athar, M Sajjad; Vacas, M J Vicente

    2011-01-01

    We study the nuclear medium effects in the weak structure functions $F_2(x,Q^2)$ and $F_3(x,Q^2)$ in the deep inelastic neutrino/antineutrino reactions in nuclei. We use a theoretical model for the nuclear spectral functions which incorporates the conventional nuclear effects, such as Fermi motion, binding and nucleon correlations. We also consider the pion and rho meson cloud contributions calculated from a microscopic model for meson-nucleus self-energies. The calculations have been performed using relativistic nuclear spectral functions which include nucleon correlations. Our results are compared with the experimental data of NuTeV and CDHSW.

  10. Solvent effects in chemistry

    CERN Document Server

    Buncel, Erwin

    2015-01-01

    This book introduces the concepts, theory and experimental knowledge concerning solvent effects on the rate and equilibrium of chemical reactions of all kinds.  It begins with basic thermodynamics and kinetics, building on this foundation to demonstrate how a more detailed understanding of these effects may be used to aid in determination of reaction mechanisms, and to aid in planning syntheses. Consideration is given to theoretical calculations (quantum chemistry, molecular dynamics, etc.), to statistical methods (chemometrics), and to modern day concerns such as ""green"" chemistry, where ut

  11. The effect of Ad

    Institute of Scientific and Technical Information of China (English)

    李小艳

    2010-01-01

    There is the trend that now people appreciate those who are slim and regard slim even thin people beautiful. The thinner a person is, the more beautiful. Women, born to pursuit beauty, try various means to follow the trend. We all watch TV, and find a lot of advertisements on diet. The effect of them is tremendous. We all know the fact that it is not at all the better mouse trap will catch mouse. The sales methods are more important. If an advertisement is very interesting and seemingly effective, people will be lured by the ad and then try some of the products.

  12. The Lazarus effect

    CERN Multimedia

    Laurent Guiraud

    1998-01-01

    Members of the RD39 collaboration stage a demonstration of the Lazarus effect in the CERN cryolab. At the LHC experiments, the front-line inner detectors - trackers - will be traversed by a mammoth thousand million million passing particles per square centimetre over the lifetimes of the experiments. After long exposure to passing particles, defects appear in the silicon and the signal is destroyed. A group of physicists at Bern University have found that at temperatures below 100 K, dead detectors apparently come back to life. They're calling their discovery the Lazarus effect after the Biblical character raised from the dead by Jesus after he had been entombed for four days.

  13. Quantum Spin Hall Effect

    Energy Technology Data Exchange (ETDEWEB)

    Bernevig, B.Andrei; Zhang, Shou-Cheng; /Stanford U., Phys. Dept.

    2010-01-15

    The quantum Hall liquid is a novel state of matter with profound emergent properties such as fractional charge and statistics. Existence of the quantum Hall effect requires breaking of the time reversal symmetry caused by an external magnetic field. In this work, we predict a quantized spin Hall effect in the absence of any magnetic field, where the intrinsic spin Hall conductance is quantized in units of 2 e/4{pi}. The degenerate quantum Landau levels are created by the spin-orbit coupling in conventional semiconductors in the presence of a strain gradient. This new state of matter has many profound correlated properties described by a topological field theory.

  14. Aaru's Awakening special effects

    OpenAIRE

    Marinó Vilhjálmsson 1986

    2013-01-01

    The project is a set of three computer graphic special effects for the 2D platformer video game Aaru’s Awakening, currently being developed by the video game developers at Lumenox. This product will be used by the developers and art director at Lumenox. This can only be run inside the environment it was developed for, which is the Unity3D game development engine. Of those three effects, only two were researched and implemented and though some research was put into the third, there was not ...

  15. Picosecond Spin Seebeck Effect

    Science.gov (United States)

    Kimling, Johannes; Choi, Gyung-Min; Brangham, Jack T.; Matalla-Wagner, Tristan; Huebner, Torsten; Kuschel, Timo; Yang, Fengyuan; Cahill, David G.

    2017-02-01

    We report time-resolved magneto-optic Kerr effect measurements of the longitudinal spin Seebeck effect in normal metal /Y3Fe5 O12 bilayers driven by an interfacial temperature difference between electrons and magnons. The measured time evolution of spin accumulation induced by laser excitation indicates transfer of angular momentum across normal metal /Y3Fe5 O12 interfaces on a picosecond time scale, too short for contributions from a bulk temperature gradient in an yttrium iron garnet. The product of spin-mixing conductance and the interfacial spin Seebeck coefficient determined is of the order of 108 A m-2 K-1 .

  16. Picosecond spin Seebeck effect

    OpenAIRE

    Kimling, Johannes; Choi, Gyung-Min; Brangham, Jack T.; Matalla-Wagner, Tristan; Huebner, Torsten; Kuschel, Timo; Yang, Fengyuan; Cahill, David G.

    2016-01-01

    We report time-resolved magneto-optic Kerr effect measurements of the longitudinal spin Seebeck effect driven by an interfacial temperature difference between itinerant electrons and magnons. The measured time-evolution of spin accumulation induced by laser-excitation indicates transfer of angular momentum across Au/Y$_3$Fe$_5$O$_{12}$ and Cu/Y$_3$Fe$_5$O$_{12}$ interfaces on a picosecond time-scale. The product of spin-mixing conductance and interfacial spin Seebeck coefficient determined is...

  17. Casimir edge effects

    CERN Document Server

    Gies, H; Gies, Holger; Klingmuller, Klaus

    2006-01-01

    We compute Casimir forces in open geometries with edges, involving parallel as well as perpendicular semi-infinite plates. We focus on Casimir configurations which are governed by a unique dimensional scaling law with a universal coefficient. With the aid of worldline numerics, we determine this coefficient for various geometries for the case of scalar-field fluctuations with Dirichlet boundary conditions. Our results facilitate an estimate of the systematic error induced by the edges of finite plates, for instance, in a standard parallel-plate experiment. The Casimir edge effects for this case can be reformulated as an increase of the effective area of the configuration.

  18. Skeletal Effects of Smoking.

    Science.gov (United States)

    Cusano, Natalie E

    2015-10-01

    Smoking is a leading cause of preventable death and disability. Smoking has long been identified as a risk factor for osteoporosis, with data showing that older smokers have decreased bone mineral density and increased fracture risk compared to nonsmokers, particularly at the hip. The increase in fracture risk in smokers is out of proportion to the effects on bone density, indicating deficits in bone quality. Advanced imaging techniques have demonstrated microarchitectural deterioration in smokers, particularly in the trabecular compartment. The mechanisms by which smoking affects skeletal health remain unclear, although multiple pathways have been proposed. Smoking cessation may at least partially reverse the adverse effects of smoking on the skeleton.

  19. The Binggeli effect

    CERN Document Server

    Biernacka, Monika; Godlowski, Wlodzimierz; Bajan, Katarzyna; Flin, Piotr

    2016-01-01

    We found the alignement of elongated clusters of BM type I and III (the excess of small values of the \\Delta\\theta angles is observed), having range till about 60Mpc/h. The first one is probably connected with the origin of supergiant galaxy, while the second one with environmental effects in clusters, originated on the long filament or plane.

  20. Antioxidant effects of carotenoids

    NARCIS (Netherlands)

    Bast, A.; Haenen, G.R.M.M.; Berg, R. van den; Berg, H. van den

    1998-01-01

    Surprisingly, neither the precise pharmacological effect nor the toxicological profile is usually established for food components. Carotenoids are no exception in this regard. Only limited insight into the pharmacology and toxicology of carotenoids exists. It is known that the antioxidant action of