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Sample records for anterograde transport requires

  1. Peri-Golgi vesicles contain retrograde but not anterograde proteins consistent with the cisternal progression model of intra-Golgi transport

    OpenAIRE

    José A Martínez-Menárguez; Prekeris, Rytis; Oorschot, Viola M J; Scheller, Richard; Slot, Jan W.; Geuze, Hans J.; Klumperman, Judith

    2001-01-01

    A cisternal progression mode of intra-Golgi transport requires that Golgi resident proteins recycle by peri-Golgi vesicles, whereas the alternative model of vesicular transport predicts anterograde cargo proteins to be present in such vesicles. We have used quantitative immuno-EM on NRK cells to distinguish peri-Golgi vesicles from other vesicles in the Golgi region. We found significant levels of the Golgi resident enzyme mannosidase II and the transport machinery proteins giantin, KDEL-rece...

  2. Calsyntenin-1 shelters APP from proteolytic processing during anterograde axonal transport

    Directory of Open Access Journals (Sweden)

    Martin Steuble

    2012-06-01

    Endocytosis of amyloid-β precursor protein (APP is thought to represent the major source of substrate for the production of the amyloidogenic Aβ peptide by the β-secretase BACE1. The irreversible nature of proteolytic cleavage implies the existence of an efficient replenishment route for APP from its sites of synthesis to the cell surface. We recently found that APP exits the trans-Golgi network in intimate association with calsyntenin-1, a transmembrane cargo-docking protein for Kinesin-1-mediated vesicular transport. Here we characterized the function of calsyntenin-1 in neuronal APP transport using selective immunoisolation of intracellular trafficking organelles, immunocytochemistry, live-imaging, and RNAi. We found that APP is co-transported with calsyntenin-1 along axons to early endosomes in the central region of growth cones in carriers that exclude the α-secretase ADAM10. Intriguingly, calsyntenin-1/APP organelles contained BACE1, suggesting premature cleavage of APP along its anterograde path. However, we found that APP contained in calsyntenin-1/APP organelles was stable. We further analyzed vesicular trafficking of APP in cultured hippocampal neurons, in which calsyntenin-1 was reduced by RNAi. We found a markedly increased co-localization of APP and ADAM10 in axons and growth cones, along with increased proteolytic processing of APP and Aβ secretion in these neurons. This suggested that the reduced capacity for calsyntenin-1-dependent APP transport resulted in mis-sorting of APP into additional axonal carriers and, therefore, the premature encounter of unprotected APP with its ectodomain proteases. In combination, our results characterize calsyntenin-1/APP organelles as carriers for sheltered anterograde axonal transport of APP.

  3. Nerve-pathways of acupoint Fengch'ih in rat by anterograde transport of HRP

    Institute of Scientific and Technical Information of China (English)

    Gang-Ming Xi; Hua-Qiao Wang; Guo-Hou He; Chao-Feng Huang; Qun-Fang Yuan; Guo-Yao Wei; Hua Li; Wen-Wen Liu; Hua-Yan Fan

    2005-01-01

    AIM: To study the nervous-pathways of Fengchih acupuncture by means of anterograde transport of aqueous solution of horseradish peroxidase (HRP).METHODS: Fifty Wistar rats were randomly divided into 1,2, 3, 4, and 5 d groups, and every group had 10 animals. HRP(30% aqueous solution) was injected into a Fengchih. Serial, transverse or capital, 40 μm sections of the cervical spinal ganglia, cervical and thoracic spinal cord segment and brain were cut on a cryotome. Sections were incubated for HRP histochemistry according to the tetramethylbenzidine (TMB). Part of the sections were counterstained with neutral red. RESULTS: After 1 d of survival times, many labeled cell bodies were found in 1-4 cervical spinal ganglia, anterior horn of 1-4 cervical spinal cord, ventromedial division of facial nucleus, accessory facial nucleus ipsilaterally. With increasing survival times, the intensity of labeled cells were slightly decreased. CONCLUSION: Fengchih may bring into full play its effect by correlation of posterior ear branch of facial nerve and anterior branch of 2-3 cervical nerve with 1-4 cervical the anterior horn of the spinal cord, ventromedial division of facial nucleus, accessory facial nucleus.

  4. Anterograde Amnesia

    Directory of Open Access Journals (Sweden)

    Serap Erdogan

    2010-08-01

    Full Text Available Memory can be divided into two categories (i.e. short term memory and long term memory according to time span. Information at our long term memory that can be remembered with conscious effort are placed in declarative memory. Information that can not be remembered conciously are placed in nondeclarative memory. The definition of anterograde amnesia is inability to generate new memories after the event causing amnesia. Episodic and semantic memories are usually unaffected among patients’ who had such amnesia. Anterograde amnesia could mostly result from head trauma but in some cases the cause could be serebrovascular events, Wernicke-Korsakoff Syndrome, santral nervous system enfections, anoxia or various substances. Medial temporal lobe and medial diencephalon are two brain regions mainly related with this condition. Medial temporal lobe is consisted of hippocampus, amygdala, parahippocampal cortex, perirhinal cortex and entorhinal cortex. Hypothalamus, thalamus, mamillary bodies and several thalamic nucleases compose medial diencephalon. Fornix and rarely serebellum damage may also play role in the development of anterograde amnesia. After the famous H.M case, who had anterograde amnesia after an epileptic surgery operation, hippocampus has been placed in the focus of memory researches. In the literature there are several reports evaluating brain tissues of amnesic patients at postmortem stage. Postmortem histological evaluations consistently revealed hippocampal neuronal loss among these patients’ brain tissues. Benzodiazepines usually cause short term anterograde amnesia. Benzodiazepine receptors are allosteric modulatory sites on gamma-aminobutyric acid-A (GABA-A receptors. GABA-A receptors composed of five subunits and anterograde amnesia emerges by means of alfa 1 subunit. Anterograde amnesia has been suggested to occur by the blocking of long term potentiation in hippocampus and piriform cortex. For the treatment of the anterograde

  5. Anterograde transport blockade precedes deficits in retrograde transport in the visual projection of the DBA/2J mouse model of glaucoma

    Directory of Open Access Journals (Sweden)

    Christine M Dengler-Crish

    2014-09-01

    Full Text Available Axonal transport deficits have been reported as an early pathology in several neurodegenerative disorders, including glaucoma. However, the progression and mechanisms of these deficits are poorly understood. Previous work suggests that anterograde transport is affected earlier and to a larger degree than retrograde transport, yet this has never been examined directly in vivo. Using combined anterograde and retrograde tract tracing methods, we examined the time-course of anterograde and retrograde transport deficits in the retinofugal projection in pre-glaucomatous (3 month-old and glaucomatous (9-13 month old DBA/2J mice. DBA/2J-Gpnmb+ mice were used as a control strain and were shown to have similar retinal ganglion cell densities as C57BL/6J control mice—a strain commonly investigated in the field of vision research. Using cholera toxin-B injections into the eye and FluoroGold injections into the superior colliculus (SC, we were able to measure anterograde and retrograde transport in the primary visual projection. In DBA/2J, anterograde transport from the retina to superior colliculus (SC was decreased by 69% in the 9-10 month-old age group, while retrograde transport was only reduced by 23% from levels seen in pre-glaucomatous mice. Despite this minor reduction, retrograde transport remained largely intact in these glaucomatous age groups until 13-months of age. These findings indicate that axonal transport deficits occur in semi-functional axons that are still connected to their brain targets. Structural persistence as determined by presence of estrogen-related receptor beta label in the superficial SC was maintained beyond time-points where reductions in retrograde transport occurred, also supporting that transport deficits may be due to physiological or functional abnormalities as opposed to overt structural loss.

  6. Transportation System Requirements Document

    International Nuclear Information System (INIS)

    This Transportation System Requirements Document (Trans-SRD) describes the functions to be performed by and the technical requirements for the Transportation System to transport spent nuclear fuel (SNF) and high-level radioactive waste (HLW) from Purchaser and Producer sites to a Civilian Radioactive Waste Management System (CRWMS) site, and between CRWMS sites. The purpose of this document is to define the system-level requirements for Transportation consistent with the CRWMS Requirement Document (CRD). These requirements include design and operations requirements to the extent they impact on the development of the physical segments of Transportation. The document also presents an overall description of Transportation, its functions, its segments, and the requirements allocated to the segments and the system-level interfaces with Transportation. The interface identification and description are published in the CRWMS Interface Specification

  7. A synthetic peptide shows retro- and anterograde neuronal transport before disrupting the chemosensation of plant-pathogenic nematodes.

    Directory of Open Access Journals (Sweden)

    Dong Wang

    Full Text Available Cyst nematodes are a group of plant pathogens each with a defined host range that cause major losses to crops including potato, soybean and sugar beet. The infective mobile stage hatches from dormant eggs and moves a short distance through the soil to plant roots, which it then invades. A novel strategy for control has recently been proposed in which the plant is able to secrete a peptide which disorientates the infective stage and prevents invasion of the pathogen. This study provides indirect evidence to support the mechanism by which one such peptide disrupts chemosensory function in nematodes. The peptide is a disulphide-constrained 7-mer with the amino acid sequence CTTMHPRLC that binds to nicotinic acetylcholine receptors. A fluorescently tagged version of this peptide with both epifluorescent and confocal microscopy was used to demonstrate that retrograde transport occurs from an aqueous environment along bare-ending primary cilia of chemoreceptive sensilla. The peptide is transported to the cell bodies of these neurons and on to a limited number of other neurons to which they connect. It appears to be localised in both neuronal processes and organelles adjacent to nuclei of some neurons suggesting it could be transported through the Golgi apparatus. The peptide takes 2.5 h to reach the neuronal cell bodies. Comparative studies established that similar but less abundant uptake occurs for Caenorhabditis elegans along its well studied dye-filling chemoreceptive neurons. Incubation in peptide solution or root-exudate from transgenic plants that secrete the peptide disrupted normal orientation of infective cyst nematodes to host root diffusate. The peptide probably undergoes transport along the dye-filling non-cholinergic chemoreceptive neurons to their synapses where it is taken up by the interneurons to which they connect. Coordinated responses to chemoreception are disrupted when the sub-set of cholinergic interneurons secrete the peptide

  8. A synthetic peptide shows retro- and anterograde neuronal transport before disrupting the chemosensation of plant-pathogenic nematodes.

    Science.gov (United States)

    Wang, Dong; Jones, Laura M; Urwin, Peter E; Atkinson, Howard J

    2011-01-01

    Cyst nematodes are a group of plant pathogens each with a defined host range that cause major losses to crops including potato, soybean and sugar beet. The infective mobile stage hatches from dormant eggs and moves a short distance through the soil to plant roots, which it then invades. A novel strategy for control has recently been proposed in which the plant is able to secrete a peptide which disorientates the infective stage and prevents invasion of the pathogen. This study provides indirect evidence to support the mechanism by which one such peptide disrupts chemosensory function in nematodes. The peptide is a disulphide-constrained 7-mer with the amino acid sequence CTTMHPRLC that binds to nicotinic acetylcholine receptors. A fluorescently tagged version of this peptide with both epifluorescent and confocal microscopy was used to demonstrate that retrograde transport occurs from an aqueous environment along bare-ending primary cilia of chemoreceptive sensilla. The peptide is transported to the cell bodies of these neurons and on to a limited number of other neurons to which they connect. It appears to be localised in both neuronal processes and organelles adjacent to nuclei of some neurons suggesting it could be transported through the Golgi apparatus. The peptide takes 2.5 h to reach the neuronal cell bodies. Comparative studies established that similar but less abundant uptake occurs for Caenorhabditis elegans along its well studied dye-filling chemoreceptive neurons. Incubation in peptide solution or root-exudate from transgenic plants that secrete the peptide disrupted normal orientation of infective cyst nematodes to host root diffusate. The peptide probably undergoes transport along the dye-filling non-cholinergic chemoreceptive neurons to their synapses where it is taken up by the interneurons to which they connect. Coordinated responses to chemoreception are disrupted when the sub-set of cholinergic interneurons secrete the peptide at synapses that

  9. Mechanism underlying the anterograde transport of the influenza A virus transmembrane proteins and genome in host cytoplasm%甲型流感病毒蛋白和遗传物质在宿主细胞质内顺向转运过程及其机制

    Institute of Scientific and Technical Information of China (English)

    池晓娟; 王松; 黄一帆; 陈吉龙

    2012-01-01

    流感病毒的蛋白和基因组在宿主细胞内能否正确地转运到相关部位,直接影响到病毒颗粒的形态发生.流感病毒跨膜蛋白(HA、NA和M2)主要通过宿主细胞的运输膜泡实现转运,而宿主细胞的蛋白转运机器参与了这一过程.新合成的流感病毒核糖核蛋白复合物(vRNPs)出核后,通过与活化的Rab 11相结合,聚集于邻近微管组织中心(MTOC)的胞内体.然后以运输小膜泡的形式,沿着MTOC的微管网络向细胞膜方向转运.跨膜蛋白和基因组在细胞质内的转运受一些宿主因子的调控,如ARHGAP21和小G蛋白Cdc42能够调节NA蛋白向细胞膜转运,Rab11协助vRNPs从MTOC向细胞膜转运.文中主要讨论新合成的流感病毒跨膜蛋白和遗传物质在宿主细胞质内的顺向转运(Anterograde transport)过程与调控.%Influenza virus assembly requires the completion of viral protein and vRNP transport to the assembly site at the plasma membrane. Therefore, efficient regulation of intracellular transport of the viral proteins and vRNPs to the surface of the host cell is especially important for virus morphogenesis. Influenza A virus uses the machineries of host cells to transport its own components including ribonucleoproteins (vRNPs) and three transmembrane proteins hemagglutinin (HA), neuraminidase (NA) and matrix 2 protein (M2). It has been shown that newly synthesized vRNPs are associated with active form of Rab 11 and accumulate at recycling endosomes adjacent to the microtubule organizing center (MTOC) following nuclear export. Subsequently, they are transported along the microtubule network toward the plasma membranes in cargo vesicles. The viral transmembrane proteins are translated on the rough endoplasmic reticulum and transported to the virus assembly site at the plasma membrane. It has been found that several host factors such as ARHGAP21 and GTPase Cdc42 are involved in regulation of intracellular trafficking of influenza A virus

  10. Global Land Transport Infrastructure Requirements

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2013-06-01

    Over the next four decades, global passenger and freight travel is expected to double over 2010 levels. In order to accommodate this growth, it is expected that the world will need to add nearly 25 million paved road lane-kilometres and 335 000 rail track kilometres. In addition, it is expected that between 45 000 square kilometres and 77 000 square kilometres of new parking spaces will be added to accommodate vehicle stock growth. These land transport infrastructure additions, when combined with operations, maintenance and repairs, are expected to cost as much as USD 45 trillion by 2050. This publication reports on the International Energy Agency’s (IEA) analysis of infrastructure requirements to support projected road and rail travel through 2050, using the IEA Mobility Model. It considers land transport infrastructure additions to support travel growth to 2050. It also considers potential savings if countries pursue “avoid and shift” policies: in this scenario, cumulative global land transport infrastructure spending could decrease as much as USD 20 trillion by 2050 over baseline projections.

  11. Anterograde and Retrograde Amnesia following Bitemporal Infarction

    Directory of Open Access Journals (Sweden)

    A. Schnider

    1994-01-01

    Full Text Available A patient suffered very severe anterograde and retrograde amnesia following infarction of both medial temporal lobes (hippocampus and adjacent cortex and the left inferior temporo-occipital area. The temporal stem and the amygdala were intact; these structures do not appear to be critical for new learning in humans. Extension of the left-sided infarct into the inferior temporo-occipital lobe, an area critically involved in visual processing, appears to be responsible for our patient's loss of remote memories.

  12. Requirements for transporting the TMI-2 core

    International Nuclear Information System (INIS)

    This report summarizes the requirements associated with the actual transport of core debris from the damaged Three Mile Island Unit 2 reactor to the Idaho National Engineering Laboratory. The requirements are discussed under six headings: accountability, security, quality, safety, environment, and transportation

  13. USMC tactical motor transport lift requirements model

    OpenAIRE

    Allen, Scott Andrew

    1995-01-01

    This thesis concentrates on developing a spreadable model that can be used by Marine logisticians in computing sustainment requirements and the resulting tactical motor transport lift requirements necessary to keep a notional sized maneuver element supported on a daily basis in the Marine Corps projected maneuver warfare environment. Sustainment computations are limited to resupplying the maneuver element with food, water, fuel, and ammunition. Using an "add-in" simulation package the plannin...

  14. Industrial Requirements for Thermodynamics and Transport Properties

    DEFF Research Database (Denmark)

    Hendriks, Eric; Kontogeorgis, Georgios; Dohrn, Ralf;

    2010-01-01

    This work reports the results of an investigation on industrial requirements for thermodynamic and transport properties carried out by the Working Party on Thermodynamic and Transport properties (http://www.wp-ttp.dk/) of the European Federation of Chemical Engineering, EFCE (http://www.efce.info......This work reports the results of an investigation on industrial requirements for thermodynamic and transport properties carried out by the Working Party on Thermodynamic and Transport properties (http://www.wp-ttp.dk/) of the European Federation of Chemical Engineering, EFCE (http......://www.efce.info/). A carefully designed questionnaire was sent to a number of key technical people in companies in the oil and gas, chemicals, and pharmaceutical/biotechnology sectors. Twenty-eight companies have provided answers which formed the basis for the analysis presented here. A number of previous reviews, specifically...... addressed to or written by industrial colleagues, are discussed initially. This provides the context of the survey and material with which the results of the survey can be compared. The results of the survey have been divided into the themes: data, models, systems, properties, education, and collaboration...

  15. A Model of Anterograde Oxygenated Lung Blood Flow in Acardia.

    Science.gov (United States)

    Marinakis, Sotirios; Burki, Marco; Abdel-Sayed, Saad; von Segesser, Ludwig Karl

    2016-01-01

    In extreme situations such as hyperacute rejection of heart transplant or major heart trauma, heart explantation and extracorporeal membrane oxygenation (ECMO) hemodynamic support might be the only means for survival. In our previous model of acardia, pulmonary artery (PA) was clamped and did not receive any anterograde blood flow. A model of anterograde PA perfusion might be necessary to avoid ischemic pulmonary damage in prolonged ECMO in acardia. The aim of this study was to describe the surgical technique and to determine the feasibility of an anterograde lung perfusion in acardia through the anastomosis of the right internal mammary artery (RIMA) to the PA. A venoarterial cardiopulmonary bypass was established in three pigs (72 ± 2.6 kg) by the transjugular insertion to the caval axis of a double-staged cannula with carotid artery return. Heart was excised and ECMO was established as previously reported. Right internal mammary artery was harvested and after measurement of its output (93.3 ± 5.8 ml/min, representing 2.17% ± 0.15% of total pump flow), it was anastomosed to PA. Right internal mammary artery anastomosis to PA is a feasible, safe, and easy to perform maneuver assuring an anterograde lung perfusion in acardia. PMID:27442854

  16. COPI vesicle transport is a common requirement for tube expansion in Drosophila.

    Directory of Open Access Journals (Sweden)

    Satish Arcot Jayaram

    Full Text Available BACKGROUND: Tube expansion defects like stenoses and atresias cause devastating human diseases. Luminal expansion during organogenesis begins to be elucidated in several systems but we still lack a mechanistic view of the process in many organs. The Drosophila tracheal respiratory system provides an amenable model to study tube size regulation. In the trachea, COPII anterograde transport of luminal proteins is required for extracellular matrix assembly and the concurrent tube expansion. PRINCIPAL FINDINGS: We identified and analyzed Drosophila COPI retrograde transport mutants with narrow tracheal tubes. gammaCOP mutants fail to efficiently secrete luminal components and assemble the luminal chitinous matrix during tracheal tube expansion. Likewise, tube extension is defective in salivary glands, where it also coincides with a failure in the luminal deposition and assembly of a distinct, transient intraluminal matrix. Drosophila gammaCOP colocalizes with cis-Golgi markers and in gammaCOP mutant embryos the ER and Golgi structures are severely disrupted. Analysis of gammaCOP and Sar1 double mutants suggests that bidirectional ER-Golgi traffic maintains the ER and Golgi compartments and is required for secretion and assembly of luminal matrixes during tube expansion. CONCLUSIONS/SIGNIFICANCE: Our results demonstrate the function of COPI components in organ morphogenesis and highlight the common role of apical secretion and assembly of transient organotypic matrices in tube expansion. Intraluminal matrices have been detected in the notochord of ascidians and zebrafish COPI mutants show defects in notochord expansion. Thus, the programmed deposition and growth of distinct luminal molds may provide distending forces during tube expansion in diverse organs.

  17. Connections of the lateral reticular nucleus to the lateral vestibular nucleus in the rat. An anterograde tracing study with Phaseolus vulgaris leucoagglutinin

    NARCIS (Netherlands)

    T.J.H. Ruigrok (Tom); M. Cella (Massimo); J. Voogd

    1995-01-01

    textabstractEfferent projections from the lateral reticular nucleus in the rat were investigated with anterograde transport of Phaseolus vulgaris leucoagglutinin. Besides the well known mossy fibre connections to the cerebellar cortex and collaterals to the cerebellar nuclei, a substantial bilateral

  18. 10 CFR 11.13 - Special requirements for transportation.

    Science.gov (United States)

    2010-01-01

    ... 10 Energy 1 2010-01-01 2010-01-01 false Special requirements for transportation. 11.13 Section 11.13 Energy NUCLEAR REGULATORY COMMISSION CRITERIA AND PROCEDURES FOR DETERMINING ELIGIBILITY FOR... Authorization § 11.13 Special requirements for transportation. (a) All individuals who, after 365 days...

  19. Salt Repository Project transportation system interface requirements: Transportation system/repository receiving facility interface requirements

    International Nuclear Information System (INIS)

    This report is a preliminary review of the interface between the transportation system and the repository receiving facility for a nuclear waste mined geologic disposal system in salt. Criteria for generic cask and facility designs are developed. These criteria are derived by examining the interfaces that occur as a result of the operations needed to receive nuclear waste at a repository. These criteria provide the basis for design of a safe, operable, practical nuclear waste receiving facility. The processing functions required to move the shipping unit from the gate into the unloading area and back to the gate for dispatch are described. Criteria for a generic receiving facility are discussed but no specific facility design is presented or evaluated. The criteria are stated in general terms to allow application to a wide variety of cask and facility designs. 9 refs., 6 figs., 4 tabs

  20. Biotinylated dextran amine anterograde tracing of the canine corticospinal tract

    Institute of Scientific and Technical Information of China (English)

    Xiao Han; Guangming Lv; Huiqun Wu; Dafeng Ji; Zhou Sun; Yaofu Li; Lemin Tang

    2012-01-01

    In this study, biotinylated dextran amine (BDA) was microinjected into the left cortical motor area of the canine brain. Fluorescence microscopy results showed that a large amount of BDA-labeled pyramidal cells were visible in the left cortical motor area after injection. In the left medulla oblongata, the BDA-labeled corticospinal tract was evenly distributed, with green fluorescence that had a clear boundary with the surrounding tissue. The BDA-positive corticospinal tract entered into the right lateral funiculus of the spinal cord and descended into the posterior part of the right lateral funiculus, close to the posterior horn, from cervical to sacral segments. There was a small amount of green fluorescence in the sacral segment. The distribution of BDA labeling in the canine central nervous system was consistent with the course of the corticospinal tract. Fluorescence labeling for BDA gradually diminished with time after injection. Our findings indicate that the BDA anterograde tracing technique can be used to visualize the localization and trajectory of the corticospinal tract in the canine central nervous system.

  1. Different mechanisms contributing to savings and anterograde interference are impaired in Parkinson’s disease

    Directory of Open Access Journals (Sweden)

    Li-Ann eLeow

    2013-02-01

    Full Text Available Reinforcement and use-dependent plasticity mechanisms have been proposed to be involved in both savings and anterograde interference in adaptation to a visuomotor rotation (c.f.Huang et al., 2011. In Parkinson’s disease (PD, dopamine dysfunction is known to impair reinforcement mechanisms, and could also affect use-dependent plasticity. Here, we assessed savings and anterograde interference in PD with an A1-B-A2 paradigm in which movement repetition was (i favored by the use of a single target, and (ii manipulated through the amount of initial training. PD patients and controls completed either limited or extended training in A1 where they adapted movement to a 30° counter-clockwise rotation of visual feedback of the movement trajectory, and then adapted to a 30° clockwise rotation in B. After subsequent washout, participants readapted to the first 30° counter-clockwise rotation in A2. Controls showed significant anterograde interference from A1 to B only after extended training, and significant A1-B-A2 savings after both limited and extended training. However, despite similar A1 adaptation to controls, PD patients showed neither anterograde interference nor savings. That extended training was necessary in controls to elicit anterograde interference but not savings suggests that savings and anterograde interference do not result from equal contributions of the same underlying mechanism(s. It is suggested that use-dependent plasticity mechanisms contributes to anterograde interference but not to savings, while reinforcement mechanisms contribute to both. As both savings and anterograde interference were impaired in PD, dopamine dysfunction in PD might impair both reinforcement and use-dependent plasticity mechanisms during adaptation to a visuomotor rotation.

  2. Ndel1-derived peptides modulate bidirectional transport of injected beads in the squid giant axon

    Directory of Open Access Journals (Sweden)

    Michal Segal

    2012-01-01

    Bidirectional transport is a key issue in cellular biology. It requires coordination between microtubule-associated molecular motors that work in opposing directions. The major retrograde and anterograde motors involved in bidirectional transport are cytoplasmic dynein and conventional kinesin, respectively. It is clear that failures in molecular motor activity bear severe consequences, especially in the nervous system. Neuronal migration may be impaired during brain development, and impaired molecular motor activity in the adult is one of the hallmarks of neurodegenerative diseases leading to neuronal cell death. The mechanisms that regulate or coordinate kinesin and dynein activity to generate bidirectional transport of the same cargo are of utmost importance. We examined how Ndel1, a cytoplasmic dynein binding protein, may regulate non-vesicular bidirectional transport. Soluble Ndel1 protein, Ndel1-derived peptides or control proteins were mixed with fluorescent beads, injected into the squid giant axon, and the bead movements were recorded using time-lapse microscopy. Automated tracking allowed for extraction and unbiased analysis of a large data set. Beads moved in both directions with a clear bias to the anterograde direction. Velocities were distributed over a broad range and were typically slower than those associated with fast vesicle transport. Ironically, the main effect of Ndel1 and its derived peptides was an enhancement of anterograde motion. We propose that they may function primarily by inhibition of dynein-dependent resistance, which suggests that both dynein and kinesin motors may remain engaged with microtubules during bidirectional transport.

  3. Chlamydomonas IFT70/CrDYF-1 Is a Core Component of IFT Particle Complex B and Is Required for Flagellar Assembly

    OpenAIRE

    Fan, Zhen-Chuan; Behal, Robert H.; Geimer, Stefan; Wang, Zhaohui; Williamson, Shana M.; Zhang, Haili; Cole, Douglas G.; Qin, Hongmin

    2010-01-01

    DYF-1 is a highly conserved protein essential for ciliogenesis in several model organisms. In Caenorhabditis elegans, DYF-1 serves as an essential activator for an anterograde motor OSM-3 of intraflagellar transport (IFT), the ciliogenesis-required motility that mediates the transport of flagellar precursors and removal of turnover products. In zebrafish and Tetrahymena DYF-1 influences the cilia tubulin posttranslational modification and may have more ubiquitous function in ciliogenesis than...

  4. Space Transportation System Availability Requirements and Its Influencing Attributes Relationships

    Science.gov (United States)

    Rhodes, Russell E.; Adams, Timothy C.; McCleskey, Carey M.

    2008-01-01

    It is important that engineering and management accept the need for an availability requirement that is derived with its influencing attributes. It is the intent of this paper to provide the visibility of relationships of these major attribute drivers (variables) to each other and the resultant system inherent availability. Also important to provide bounds of the variables providing engineering the insight required to control the system's engineering solution, e.g., these influencing attributes become design requirements also. These variables will drive the need to provide integration of similar discipline functions or technology selection to allow control of the total parts count. The relationship of selecting a reliability requirement will place a constraint on parts count to achieve a given availability requirement or if allowed to increase the parts count will drive the system reliability requirement higher. They also provide the understanding for the relationship of mean repair time (or mean down time) to maintainability, e.g., accessibility for repair, and both the mean time between failure, e.g., reliability of hardware and availability. The concerns and importance of achieving a strong availability requirement is driven by the need for affordability, the choice of using the two launch solution for the single space application, or the need to control the spare parts count needed to support the long stay in either orbit or on the surface of the moon. Understanding the requirements before starting the architectural design concept will avoid considerable time and money required to iterate the design to meet the redesign and assessment process required to achieve the results required of the customer's space transportation system. In fact the impact to the schedule to being able to deliver the system that meets the customer's needs, goals, and objectives may cause the customer to compromise his desired operational goal and objectives resulting in considerable

  5. Space Transportation System Availability Requirement and Its Influencing Attributes Relationships

    Science.gov (United States)

    Rhodes, Russell E.; Adams, Timothy C.; McCleskey, Carey M.

    2008-01-01

    It is important that engineering and management accept the need for an availability requirement that is derived with its influencing attributes. It is the intent of this paper to provide the visibility of relationships of these major attribute drivers (variables) to each other and the resultant system inherent availability. Also important to provide bounds of the variables providing engineering the insight required to control the system's engineering solution, e.g., these influencing attributes become design requirements also. These variables will drive the need to provide integration of similar discipline functions or technology selection to allow control of the total parts count. The relationship of selecting a reliability requirement will place a constraint on parts count to achieve a given availability requirement or if allowed to increase the parts count will drive the system reliability requirement higher. They also provide the understanding for the relationship of mean repair time (or mean down time) to maintainability, e.g., accessibility for repair, and both the mean time between failure, e.g., reliability of hardware and availability. The concerns and importance of achieving a strong availability requirement is driven by the need for affordability, the choice of using the two launch solution for the single space application, or the need to control the spare parts count needed to support the long stay in either orbit or on the surface of the moon. Understanding the requirements before starting the architectural design concept will avoid considerable time and money required to iterate the design to meet the redesign and assessment process required to achieve the results required of the customer's space transportation system. In fact the impact to the schedule to being able to deliver the system that meets the customer's needs, goals, and objectives may cause the customer to compromise his desired operational goal and objectives resulting in considerable

  6. Analysis of near-term spent fuel transportation hardware requirements and transportation costs

    International Nuclear Information System (INIS)

    A computer model was developed to quantify the transportation hardware requirements and transportation costs associated with shipping spent fuel in the commercial nucler fuel cycle in the near future. Results from this study indicate that alternative spent fuel shipping systems (consolidated or disassembled fuel elements and new casks designed for older fuel) will significantly reduce the transportation hardware requirements and costs for shipping spent fuel in the commercial nuclear fuel cycle, if there is no significant change in their operating/handling characteristics. It was also found that a more modest cost reduction results from increasing the fraction of spent fuel shipped by truck from 25% to 50%. Larger transportation cost reductions could be realized with further increases in the truck shipping fraction. Using the given set of assumptions, it was found that the existing spent fuel cask fleet size is generally adequate to perform the needed transportation services until a fuel reprocessing plant (FRP) begins to receive fuel (assumed in 1987). Once the FRP opens, up to 7 additional truck systems and 16 additional rail systems are required at the reference truck shipping fraction of 25%. For the 50% truck shipping fraction, 17 additional truck systems and 9 additional rail systems are required. If consolidated fuel only is shipped (25% by truck), 5 additional rail casks are required and the current truck cask fleet is more than adequate until at least 1995. Changes in assumptions could affect the results. Transportation costs for a federal interim storage program could total about $25M if the FRP begins receiving fuel in 1987 or about $95M if the FRP is delayed until 1989. This is due to an increased utilization of federal interim storage facility from 350 MTU for the reference scenario to about 750 MTU if reprocessing is delayed by two years

  7. Space Transportation System Availability Requirements and Its Influencing Attributes Relationships

    Science.gov (United States)

    Rhodes, Russel E.; Adams, TImothy C.

    2008-01-01

    It is essential that management and engineering understand the need for an availability requirement for the customer's space transportation system as it enables the meeting of his needs, goal, and objectives. There are three types of availability, e.g., operational availability, achieved availability, or inherent availability. The basic definition of availability is equal to the mean uptime divided by the sum of the mean uptime plus the mean downtime. The major difference is the inclusiveness of the functions within the mean downtime and the mean uptime. This paper will address tIe inherent availability which only addresses the mean downtime as that mean time to repair or the time to determine the failed article, remove it, install a replacement article and verify the functionality of the repaired system. The definitions of operational availability include the replacement hardware supply or maintenance delays and other non-design factors in the mean downtime. Also with inherent availability the mean uptime will only consider the mean time between failures (other availability definitions consider this as mean time between maintenance - preventive and corrective maintenance) that requires the repair of the system to be functional. It is also essential that management and engineering understand all influencing attributes relationships to each other and to the resultant inherent availability requirement. This visibility will provide the decision makers with the understanding necessary to place constraints on the design definition for the major drivers that will determine the inherent availability, safety, reliability, maintainability, and the life cycle cost of the fielded system provided the customer. This inherent availability requirement may be driven by the need to use a multiple launch approach to placing humans on the moon or the desire to control the number of spare parts required to support long stays in either orbit or on the surface of the moon or mars. It is

  8. Differences of Technical Requirements Between Transportation and Storage Metal Casks

    International Nuclear Information System (INIS)

    The worldwide demand of storage facilities for spent fuels discharged from nuclear power stations is increasing to maintain sustainable operation of the nuclear power stations. The spent fuels are stored at first in the fuel pools (wet storage). When the spent fuels exceed the pool storage capacity, the fuels are transferred to the other storage facility located at reactor or away from reactor, which often adopts a dry storage technology. To use metal casks is one of the options for the dry storage facilities, and some storage facilities have already utilized large metal casks, whose original design concept were developed to transport the spent fuels from nuclear power stations to a reprocessing plant by trains, trucks or by sea-going vessels. It is widely understood that the technology of transportation casks developed up to now is able to apply to the storage casks without any significant design changes. Technical requirements on the design are discussed between the storage cask and the transportation cask to confirm of the understanding based on the assumption that the large metal cask is used for transportation and storage respectively. (author)

  9. Physical and transportation requirements for a FLIP fueled TRIGA

    International Nuclear Information System (INIS)

    Several major changes to the OSTR Physical Security Plan were required by the NRC prior to the August 1976 receipt and installation of a new core consisting entirely of FLIP fuel. The general nature of these changes will be reviewed along with several decisions we faced during their implementation. At the previous TRIGA Owners' Conference in Salt Lake City, Utah, we reported on Oregon's regulatory program for research reactor emergency response planning and physical security. The latter program was of particular interest to us in light of the projected FLIP fuel shipments. The impact of the State's program for physical security of FLIP fuel during transportation will be presented. (author)

  10. Anterograde amnesia as a possible postoperative complication of Midazolam as an agent for intravenous conscious sedation.

    Science.gov (United States)

    Malamed, S F; Nikchevich, D; Block, J

    1988-01-01

    Anterograde amnesia is often considered to be a beneficial effect of intravenous conscious sedation. The recently introduced benzodiazepine, midazolam, has associated with its administration a significant anterograde amnesic period. In the case presented here, a healthy young female presented for third molar extraction under midazolam conscious sedation and local anesthesia. After uncomplicated removal of the teeth and clinically adequate recovery from sedation, it was noted that the patient had swallowed the postsurgical gauze packs. Efforts at recovery of the gauze packs were futile. Follow-up discussion with the patient revealed a complete lack of recall of all events occurring for up to an hour or more after the administration of intravenous midazolam. The need for written and oral postoperative instructions to both the patient and his/her escort is emphasized.

  11. Hypothermia-induced anterograde amnesia: is memory loss attributable to impaired acquisition?

    Science.gov (United States)

    Santucci, A C; Kasenow, P M; Riccio, D C; Richardson, R

    1987-07-01

    The present investigation examined whether the poor test performance observed in studies of anterograde amnesia reflects a memory deficit or is a by-product of weaker initial learning resulting from impaired sensory, motivational, or associative processes. Two experiments were performed which utilized latent extinction (Experiment 1) and delay of punishment (Experiment 2) manipulations in order to assess the nature of original learning in rats trained under either hypothermic (29 degrees C) or normothermic conditions. Results from both experiments provided evidence that hypothermia treatment administered prior to training had relatively little influence on the animal's ability to acquire a passive avoidance response. Therefore, the rapid forgetting observed in hypothermia-induced anterograde amnesia is most likely due to memory deficits rather than an artifact of poorer acquisition. PMID:3632548

  12. ARF Is Required for Maintenance of Yeast Golgi and Endosome Structure and Function

    OpenAIRE

    Gaynor, Erin C.; Chen, Chih-Ying; Emr, Scott D.; Graham, Todd R.

    1998-01-01

    ADP ribosylation factor (ARF) is thought to play a critical role in recruiting coatomer (COPI) to Golgi membranes to drive transport vesicle budding. Yeast strains harboring mutant COPI proteins exhibit defects in retrograde Golgi to endoplasmic reticulum protein transport and striking cargo-selective defects in anterograde endoplasmic reticulum to Golgi protein transport. To determine whether arf mutants exhibit similar phenotypes, the anterograde transport kinetics of multiple cargo protein...

  13. Anterograde jejunojejunal intussusception resulted in acute efferent loop syndrome after subtotal gastrectomy

    Institute of Scientific and Technical Information of China (English)

    Jung; Myun; Kwak; Jin; Kim; Sung; Ock; Suh

    2010-01-01

    Postoperative intussusception is an unusual clinical entity in adults,and is rarely encountered as a complication following gastric surgery.The most common type after gastric surgery is retrograde jejunogastric intussusception,and jejunojejunal intussusception has been rarely reported.We report a case of anterograde jejunojejunal intussusception after radical subtotal gastrectomy with Billroth Ⅱ anastomosis in a 38-year-old Korean woman with early gastric cancer,and include a review of the literature on thi...

  14. 19 CFR 122.117 - Requirements for transit air cargo transport.

    Science.gov (United States)

    2010-04-01

    ... 19 Customs Duties 1 2010-04-01 2010-04-01 false Requirements for transit air cargo transport. 122... Requirements for transit air cargo transport. (a) Transportation—(1) Port to port. Transit air cargo may be... cargo, a receipt shall be given. The receipt shall be made by the airline responsible for transport...

  15. Key requirements of packet transport network based on MPLS-TP

    Science.gov (United States)

    Huang, Feng; Yi, Xiaobo; Zhang, Hanzheng; Gong, Ping

    2009-11-01

    Requirement of packet transport network based on MPLS-TP are analyzed including in transport plane, OAM, survivability, QoS, control plane and management plane. MPLS-TP standard status is also introduced.

  16. Transport of radioactive materials and equipment. Requirements. (Provisional)

    International Nuclear Information System (INIS)

    This standard is aimed at establishing the procedures that must be followed when transporting radioactive materials and equipment in Venezuelan Territory. The ''Consejo Nacional para el Desarrollo de la Industria Nuclear'' is responsible for their fulfillment and control

  17. Dopamine transporter inhibition is required for cocaine-induced stereotypy

    OpenAIRE

    Tilley, Michael R.; Gu, Howard H.

    2008-01-01

    The primary mechanism by which cocaine induces stereotypy has been difficult to discern because cocaine has three high affinity targets, the reuptake transporters for dopamine (DAT), norepinephrine, and serotonin. To dissect out the role of DAT in cocaine effects, we generated a knock-in mouse line with a cocaine insensitive DAT (DAT-CI mice). DAT-CI mice provide a powerful tool that can directly test whether DAT inhibition is important for cocaine-induced stereotypy. We found that acute coca...

  18. Anterograde trafficking of KCa3.1 in polarized epithelia is Rab1- and Rab8-dependent and recycling endosome-independent.

    Directory of Open Access Journals (Sweden)

    Claudia A Bertuccio

    Full Text Available The intermediate conductance, Ca2+-activated K+ channel (KCa3.1 targets to the basolateral (BL membrane in polarized epithelia where it plays a key role in transepithelial ion transport. However, there are no studies defining the anterograde and retrograde trafficking of KCa3.1 in polarized epithelia. Herein, we utilize Biotin Ligase Acceptor Peptide (BLAP-tagged KCa3.1 to address these trafficking steps in polarized epithelia, using MDCK, Caco-2 and FRT cells. We demonstrate that KCa3.1 is exclusively targeted to the BL membrane in these cells when grown on filter supports. Following endocytosis, KCa3.1 degradation is prevented by inhibition of lysosomal/proteosomal pathways. Further, the ubiquitylation of KCa3.1 is increased following endocytosis from the BL membrane and PR-619, a deubiquitylase inhibitor, prevents degradation, indicating KCa3.1 is targeted for degradation by ubiquitylation. We demonstrate that KCa3.1 is targeted to the BL membrane in polarized LLC-PK1 cells which lack the μ1B subunit of the AP-1 complex, indicating BL targeting of KCa3.1 is independent of μ1B. As Rabs 1, 2, 6 and 8 play roles in ER/Golgi exit and trafficking of proteins to the BL membrane, we evaluated the role of these Rabs in the trafficking of KCa3.1. In the presence of dominant negative Rab1 or Rab8, KCa3.1 cell surface expression was significantly reduced, whereas Rabs 2 and 6 had no effect. We also co-immunoprecipitated KCa3.1 with both Rab1 and Rab8. These results suggest these Rabs are necessary for the anterograde trafficking of KCa3.1. Finally, we determined whether KCa3.1 traffics directly to the BL membrane or through recycling endosomes in MDCK cells. For these studies, we used either recycling endosome ablation or dominant negative RME-1 constructs and determined that KCa3.1 is trafficked directly to the BL membrane rather than via recycling endosomes. These results are the first to describe the anterograde and retrograde trafficking of KCa3

  19. Greater-than-Class C low-level radioactive waste transportation regulations and requirements study

    International Nuclear Information System (INIS)

    The purpose of this report is to identify the regulations and requirements for transporting greater-than-Class C (GTCC) low-level radioactive waste (LLW) and to identify planning activities that need to be accomplished in preparation for transporting GTCC LLW. The regulations and requirements for transporting hazardous materials, of which GTCC LLW is included, are complex and include several Federal agencies, state and local governments, and Indian tribes. This report is divided into five sections and three appendices. Section 1 introduces the report. Section 2 identifies and discusses the transportation regulations and requirements. The regulations and requirements are divided into Federal, state, local government, and Indian tribes subsections. This report does not identify the regulations or requirements of specific state, local government, and Indian tribes, since the storage, treatment, and disposal facility locations and transportation routes have not been specifically identified. Section 3 identifies the planning needed to ensure that all transportation activities are in compliance with the regulations and requirements. It is divided into (a) transportation packaging; (b) transportation operations; (c) system safety and risk analysis, (d) route selection; (e) emergency preparedness and response; and (f) safeguards and security. This section does not provide actual planning since the details of the Department of Energy (DOE) GTCC LLW Program have not been finalized, e.g., waste characterization and quantity, storage, treatment and disposal facility locations, and acceptance criteria. Sections 4 and 5 provide conclusions and referenced documents, respectively

  20. Functional requirements for the Automated Transportation Management System: TTP number: RL 439002

    Energy Technology Data Exchange (ETDEWEB)

    Portsmouth, J.H. [Westinghouse Hanford Co., Richland, WA (United States)

    1992-12-31

    This requirements analysis, documents Department of Energy (DOE) transportation management procedures for the purpose of providing a clear and mutual understanding between users and designers of the proposed Automated Transportation Management System (ATMS). It is imperative that one understand precisely how DOE currently performs traffic management tasks; only then can an integrated system be proposed that successfully satisfies the major requirements of transportation managers and other system users. Accordingly, this report describes the current workings of DOE transportation organizations and then proposes a new system which represents a synthesis of procedures (both current and desired) which forms the basis for further systems development activities.

  1. Can postictal suppression of the perforant path - fascia dentata responses account for the ECS-induced anterograde amnesia in rats?

    Science.gov (United States)

    Peréz-Saad, H; Valousková, V; Bures, J

    1984-07-15

    Electroconvulsive shock (ECS) decreases fascia dentata responses to entorhinal stimulation by 50% in unanesthetized rats. Synaptic potentials and population spikes return to pre-ECS level during 1 h and 3 h, respectively. This recovery rate is compared with the dynamics of ECS-induced anterograde amnesia.

  2. 30 CFR 75.1106-2 - Transportation of liquefied and nonliquefied compressed gas cylinders; requirements.

    Science.gov (United States)

    2010-07-01

    ... Fire Protection § 75.1106-2 Transportation of liquefied and nonliquefied compressed gas cylinders... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Transportation of liquefied and nonliquefied compressed gas cylinders; requirements. 75.1106-2 Section 75.1106-2 Mineral Resources MINE SAFETY AND...

  3. Anterograde effects of a single electroconvulsive shock on inhibitory avoidance and on cued fear conditioning

    Directory of Open Access Journals (Sweden)

    Oliveira M.G.M.

    1998-01-01

    Full Text Available A single electroconvulsive shock (ECS or a sham ECS was administered to male 3-4-month-old Wistar rats 1, 2, and 4 h before training in an inhibitory avoidance test and in cued classical fear conditioning (measured by means of freezing time in a new environment. ECS impaired inhibitory avoidance at all times and, at 1 or 2 h before training, reduced freezing time before and after re-presentation of the ECS. These results are interpreted as a transient conditioned stimulus (CS-induced anxiolytic or analgesic effect lasting about 2 h after a single treatment, in addition to the known amnesic effect of the stimulus. This suggests that the effect of anterograde learning impairment is demonstrated unequivocally only when the analgesic/anxiolytic effect is over (about 4 h after ECS administration and that this impairment of learning is selective, affecting inhibitory avoidance but not classical fear conditioning to a discrete stimulus.

  4. Anterograde removal of broken femoral nails without opening the nonunion site: a new technique

    Directory of Open Access Journals (Sweden)

    Henrique Antônio Berwanger de Amorim Cabrita

    2010-01-01

    Full Text Available OBJECTIVE: We describe a new technique for removing the distal fragments of broken intramedullary femoral nails without disturbing the nonunion site. METHODS: This technique involves the application of an AO distractor prior to the removal of the nail fragments, with subsequent removal of the proximal nail fragment in an anterograde fashion and removal of the distal fragment through a medial parapatellar approach. Impaction of the fracture site is then performed with a nail that is broader than the remaining fragmented material. RESULTS: Nails were removed from five patients using the technique described above without any complications. After a mean follow-up period of 61.8 months, none of these patients showed worsened knee osteoarthritis. CONCLUSION: The original technique described in this article allows surgeons to remove the distal fragment of fractured femoral intramedullary nails without opening the nonunion focus or using special surgical instruments.

  5. Unexpected anterograde amnesia associated with Buscopan used as a predmedication for endocscopy

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    It has been known that peripheral adverse event is caused by peripheral antimuscarinic action, from hyoscine butylbromide (Buscopan; Boehringer Ingelheim, Germany)used as a premedication for endoscopy. However,symptoms or signs associated with the central nervous system are rarely reported in the field of anesthesiology and peripartum labor. This central anticholinergic syndrome is likely caused by blockade of muscarinic cholinergic receptors in the central nervous system. There is no report on Buscopan-induced central anticholinergic syndrome in endoscopy room so far. Three middle-aged females unexpectedly suffered from anterograde amnesia after intramuscular injection of hyoscine butylbromide as an antispasmodic premedication for endoscopy at our endoscopy unit in the Health Promotion Center.

  6. Capital requirements for the transportation of energy materials: 1979 arc estimates

    Energy Technology Data Exchange (ETDEWEB)

    1980-08-29

    Summaries of transportation investment requirements through 1990 are given for the low, medium and high scenarios. Total investment requirements for the three modes and the three energy commodities can accumulate to a $46.3 to $47.0 billion range depending on the scenario. The high price of oil, following the evidence of the last year, is projected to hold demand for oil below the recent past. Despite the overall decrease in traffic some investment in crude oil and LPG pipelines is necessary to reach new sources of supply. Although natural gas production and consumption is projected to decline through 1990, new investments in carrying capacity also are required due to locational shifts in supply. The Alaska Natural Gas Transportation System is the dominant investment for energy transportation in the next ten years. This year's report focuses attention on waterborne coal transportation to the northeast states in keeping with a return to significant coal consumption projected for this area. A resumption of such shipments will require a completely new fleet. The investment estimates given in this report identify capital required to transport projected energy supplies to market. The requirement is strategic in the sense that other reasonable alternatives do not exist or that a shared load of new growth can be expected. Not analyzed or forecasted are investments in transportation facilities made in response to local conditions. The total investment figures, therefore, represent a minimum necessary capital improvement to respond to changes in interregional supply conditions.

  7. Anterograde Activin signaling regulates postsynaptic membrane potential and GluRIIA/B abundance at the Drosophila neuromuscular junction.

    Directory of Open Access Journals (Sweden)

    Myung-Jun Kim

    Full Text Available Members of the TGF-β superfamily play numerous roles in nervous system development and function. In Drosophila, retrograde BMP signaling at the neuromuscular junction (NMJ is required presynaptically for proper synapse growth and neurotransmitter release. In this study, we analyzed whether the Activin branch of the TGF-β superfamily also contributes to NMJ development and function. We find that elimination of the Activin/TGF-β type I receptor babo, or its downstream signal transducer smox, does not affect presynaptic NMJ growth or evoked excitatory junctional potentials (EJPs, but instead results in a number of postsynaptic defects including depolarized membrane potential, small size and frequency of miniature excitatory junction potentials (mEJPs, and decreased synaptic densities of the glutamate receptors GluRIIA and B. The majority of the defective smox synaptic phenotypes were rescued by muscle-specific expression of a smox transgene. Furthermore, a mutation in actβ, an Activin-like ligand that is strongly expressed in motor neurons, phenocopies babo and smox loss-of-function alleles. Our results demonstrate that anterograde Activin/TGF-β signaling at the Drosophila NMJ is crucial for achieving normal abundance and localization of several important postsynaptic signaling molecules and for regulating postsynaptic membrane physiology. Together with the well-established presynaptic role of the retrograde BMP signaling, our findings indicate that the two branches of the TGF-β superfamily are differentially deployed on each side of the Drosophila NMJ synapse to regulate distinct aspects of its development and function.

  8. The Arabidopsis Golgi-localized GDP-L-fucose transporter is required for plant development.

    Science.gov (United States)

    Rautengarten, Carsten; Ebert, Berit; Liu, Lifeng; Stonebloom, Solomon; Smith-Moritz, Andreia M; Pauly, Markus; Orellana, Ariel; Scheller, Henrik Vibe; Heazlewood, Joshua L

    2016-01-01

    Nucleotide sugar transport across Golgi membranes is essential for the luminal biosynthesis of glycan structures. Here we identify GDP-fucose transporter 1 (GFT1), an Arabidopsis nucleotide sugar transporter that translocates GDP-L-fucose into the Golgi lumen. Using proteo-liposome-based transport assays, we show that GFT preferentially transports GDP-L-fucose over other nucleotide sugars in vitro, while GFT1-silenced plants are almost devoid of L-fucose in cell wall-derived xyloglucan and rhamnogalacturonan II. Furthermore, these lines display reduced L-fucose content in N-glycan structures accompanied by severe developmental growth defects. We conclude that GFT1 is the major nucleotide sugar transporter for import of GDP-L-fucose into the Golgi and is required for proper plant growth and development. PMID:27381418

  9. The Arabidopsis Golgi-localized GDP-L-fucose transporter is required for plant development.

    Science.gov (United States)

    Rautengarten, Carsten; Ebert, Berit; Liu, Lifeng; Stonebloom, Solomon; Smith-Moritz, Andreia M; Pauly, Markus; Orellana, Ariel; Scheller, Henrik Vibe; Heazlewood, Joshua L

    2016-07-06

    Nucleotide sugar transport across Golgi membranes is essential for the luminal biosynthesis of glycan structures. Here we identify GDP-fucose transporter 1 (GFT1), an Arabidopsis nucleotide sugar transporter that translocates GDP-L-fucose into the Golgi lumen. Using proteo-liposome-based transport assays, we show that GFT preferentially transports GDP-L-fucose over other nucleotide sugars in vitro, while GFT1-silenced plants are almost devoid of L-fucose in cell wall-derived xyloglucan and rhamnogalacturonan II. Furthermore, these lines display reduced L-fucose content in N-glycan structures accompanied by severe developmental growth defects. We conclude that GFT1 is the major nucleotide sugar transporter for import of GDP-L-fucose into the Golgi and is required for proper plant growth and development.

  10. Programmatic and technical requirements for the FMDP fresh MOX fuel transport package

    International Nuclear Information System (INIS)

    This document is intended to guide the designers of the package to all pertinent regulatory and other design requirements to help ensure the safe and efficient transport of the weapons-grade (WG) fresh MOX fuel under the Fissile Materials Disposition Program. To accomplish the disposition mission using MOX fuel, the unirradiated MOX fuel must be transported from the MOX fabrication facility to one or more commercial reactors. Because the unirradiated fuel contains large quantities of plutonium and is not sufficient radioactive to create a self-protecting barrier to deter the material from theft, DOE intends to use its fleet of safe secure trailers (SSTs) to provide the necessary safeguards and security for the material in transit. In addition to these requirements, transport of radioactive materials must comply with regulations of the Department of Transportation and the Nuclear Regulatory Commission (NRC). In particular, NRC requires that the packages must meet strict performance requirements. The requirements for shipment of MOX fuel (i.e., radioactive fissile materials) specify that the package design is certified by NRC to ensure the materials contained in the packages are not released and remain subcritical after undergoing a series of hypothetical accident condition tests. Packages that pass these tests are certified by NRC as a Type B fissile (BF) package. This document specifies the programmatic and technical design requirements a package must satisfy to transport the fresh MOX fuel assemblies

  11. Programmatic and technical requirements for the FMDP fresh MOX fuel transport package

    Energy Technology Data Exchange (ETDEWEB)

    Ludwig, S. B.; Michelhaugh, R. D.; Pope, R. B.; Shappert, L. B.; Singletary, B. H.; Chae, S. M.; Parks, C. V.; Broadhead, B. L.; Schmid, S. P.; Cowart, C. G.

    1997-12-01

    This document is intended to guide the designers of the package to all pertinent regulatory and other design requirements to help ensure the safe and efficient transport of the weapons-grade (WG) fresh MOX fuel under the Fissile Materials Disposition Program. To accomplish the disposition mission using MOX fuel, the unirradiated MOX fuel must be transported from the MOX fabrication facility to one or more commercial reactors. Because the unirradiated fuel contains large quantities of plutonium and is not sufficient radioactive to create a self-protecting barrier to deter the material from theft, DOE intends to use its fleet of safe secure trailers (SSTs) to provide the necessary safeguards and security for the material in transit. In addition to these requirements, transport of radioactive materials must comply with regulations of the Department of Transportation and the Nuclear Regulatory Commission (NRC). In particular, NRC requires that the packages must meet strict performance requirements. The requirements for shipment of MOX fuel (i.e., radioactive fissile materials) specify that the package design is certified by NRC to ensure the materials contained in the packages are not released and remain subcritical after undergoing a series of hypothetical accident condition tests. Packages that pass these tests are certified by NRC as a Type B fissile (BF) package. This document specifies the programmatic and technical design requirements a package must satisfy to transport the fresh MOX fuel assemblies.

  12. 78 FR 17781 - Transportation Worker Identification Credential (TWIC)-Reader Requirements

    Science.gov (United States)

    2013-03-22

    ... vessels and facilities. This is consistent with the understanding that TWIC readers constitute one.... Transportation Worker Identification Credential (TWIC)--Reader Requirements; Proposed Rule #0;#0;Federal Register... Credential (TWIC)--Reader Requirements AGENCY: Coast Guard, DHS. ACTION: Notice of proposed...

  13. A hereditary spastic paraplegia mutation in kinesin-1A/KIF5A disrupts neurofilament transport

    Directory of Open Access Journals (Sweden)

    Brown Anthony

    2010-11-01

    Full Text Available Abstract Background Hereditary spastic paraplegias are a group of neurological disorders characterized by progressive distal degeneration of the longest ascending and descending axons in the spinal cord, leading to lower limb spasticity and weakness. One of the dominantly inherited forms of this disease (spastic gait type 10, or SPG10 is caused by point mutations in kinesin-1A (also known as KIF5A, which is thought to be an anterograde motor for neurofilaments. Results We investigated the effect of an SPG10 mutation in kinesin-1A (N256S-kinesin-1A on neurofilament transport in cultured mouse cortical neurons using live-cell fluorescent imaging. N256S-kinesin-1A decreased both anterograde and retrograde neurofilament transport flux by decreasing the frequency of anterograde and retrograde movements. Anterograde velocity was not affected, whereas retrograde velocity actually increased. Conclusions These data reveal subtle complexities to the functional interdependence of the anterograde and retrograde neurofilament motors and they also raise the possibility that anterograde and retrograde neurofilament transport may be disrupted in patients with SPG10.

  14. Establishing Bedding Requirements during Transport and Monitoring Skin Temperature during Cold and Mild Seasons after Transport for Finishing Pigs

    Directory of Open Access Journals (Sweden)

    John McGlone

    2014-05-01

    Full Text Available The broad aim of this study was to determine whether bedding level in the transport trailer influenced pig performance and welfare. Specifically, the objective was to define the bedding requirements of pigs during transportation in commercial settings during cold and mild weather. Animals (n = 112,078 pigs on 572 trailers used were raised in commercial finishing sites and transported in trailers to commercial processing plants. Dead on arrival (DOA, non-ambulatory (NA, and total dead and down (D&D data were collected and skin surface temperatures of the pigs were measured by infrared thermography. Data were collected during winter (Experiment 1 and fall/spring (Experiment 2. Total D&D percent showed no interaction between bedding level and outside air temperature in any experiments. Average skin surface temperature during unloading increased with outside air temperature linearly in both experiments (P < 0.01. In conclusion, over-use of bedding may be economically inefficient. Pig skin surface temperature could be a useful measure of pig welfare during or after transport.

  15. Retrosplenial Cortical Contributions to Anterograde and Retrograde Memory in the Monkey.

    Science.gov (United States)

    Buckley, Mark J; Mitchell, Anna S

    2016-06-01

    Primate retrosplenial cortex (RSC) is important for memory but patient neuropathologies are diffuse so its key contributions to memory remain elusive. This study provides the first causal evidence that RSC in macaque monkeys is crucial for postoperative retention of preoperatively and postoperatively acquired memories. Preoperatively, monkeys learned 300 object-in-place scene discriminations across sessions. After RSC removal, one-trial postoperative retention tests revealed significant retrograde memory loss for these 300 discriminations relative to unoperated control monkeys. Less robust evidence was found for a deficit in anterograde memory (new postoperative learning) after RSC lesions as new learning to criterion measures failed to reveal any significant learning impairment. However, after achieving ≥90% learning criterion for the postoperatively presented novel 100 object-in-place scene discriminations, short-term retention (i.e., measured after 24 h delay) of this well-learnt set was impaired in the RSC monkeys relative to controls. A further experiment assessed rapid "within" session acquisition of novel object-in-place scene discriminations, again confirming that new learning per se was unimpaired by bilateral RSC removal. Primate RSC contributes critically to memory by supporting normal retention of information, even when this information does not involve an autobiographical component. PMID:26946129

  16. Retrosplenial Cortical Contributions to Anterograde and Retrograde Memory in the Monkey.

    Science.gov (United States)

    Buckley, Mark J; Mitchell, Anna S

    2016-06-01

    Primate retrosplenial cortex (RSC) is important for memory but patient neuropathologies are diffuse so its key contributions to memory remain elusive. This study provides the first causal evidence that RSC in macaque monkeys is crucial for postoperative retention of preoperatively and postoperatively acquired memories. Preoperatively, monkeys learned 300 object-in-place scene discriminations across sessions. After RSC removal, one-trial postoperative retention tests revealed significant retrograde memory loss for these 300 discriminations relative to unoperated control monkeys. Less robust evidence was found for a deficit in anterograde memory (new postoperative learning) after RSC lesions as new learning to criterion measures failed to reveal any significant learning impairment. However, after achieving ≥90% learning criterion for the postoperatively presented novel 100 object-in-place scene discriminations, short-term retention (i.e., measured after 24 h delay) of this well-learnt set was impaired in the RSC monkeys relative to controls. A further experiment assessed rapid "within" session acquisition of novel object-in-place scene discriminations, again confirming that new learning per se was unimpaired by bilateral RSC removal. Primate RSC contributes critically to memory by supporting normal retention of information, even when this information does not involve an autobiographical component.

  17. 49 CFR 173.427 - Transport requirements for low specific activity (LSA) Class 7 (radioactive) materials and...

    Science.gov (United States)

    2010-10-01

    ... this section must be packaged in accordance with 10 CFR part 71. (e) Tables 5 and 6 are as follows... 49 Transportation 2 2010-10-01 2010-10-01 false Transport requirements for low specific activity... SHIPMENTS AND PACKAGINGS Class 7 (Radioactive) Materials § 173.427 Transport requirements for low...

  18. Transition fibre protein FBF1 is required for the ciliary entry of assembled intraflagellar transport complexes

    OpenAIRE

    WEI, QING; Xu, Qingwen; Zhang, Yuxia; Li, Yujie; Zhang, Qing; Hu, Zeng; Harris, Peter C.; Torres, Vicente E.; Ling, Kun; Hu, Jinghua

    2013-01-01

    Sensory organelle cilia play critical roles in mammalian embryonic development and tissue homeostasis. Intraflagellar transport (IFT) machinery is required for the assembly and maintenance of cilia. Yet how this large complex passes through the size-dependent barrier at the ciliary base remains enigmatic. Here we report that FBF1, a highly conserved transition fibre protein, is required for the ciliary import of assembled IFT particles at the cilia base. We cloned dyf-19, the C. elegans homol...

  19. 76 FR 14643 - Hazardous Materials: Safety Requirements for External Product Piping on Cargo Tanks Transporting...

    Science.gov (United States)

    2011-03-17

    ... April 11, 2000 (65 FR 19477) or you may visit http://www.regulations.gov . SUPPLEMENTARY INFORMATION: I. Background On January 27, 2011, PHMSA published a notice of proposed rulemaking (NPRM) (HM-213D; 76 FR 4847...: Safety Requirements for External Product Piping on Cargo Tanks Transporting Flammable Liquids...

  20. 40 CFR 80.350 - What alternative sulfur standards and requirements apply to importers who transport gasoline by...

    Science.gov (United States)

    2010-07-01

    ... requirements apply to importers who transport gasoline by truck? 80.350 Section 80.350 Protection of... ADDITIVES Gasoline Sulfur Sampling, Testing and Retention Requirements for Refiners and Importers § 80.350 What alternative sulfur standards and requirements apply to importers who transport gasoline by...

  1. Capital requirements for the transportation of energy materials: 1979 ARC estimates. Final report

    Energy Technology Data Exchange (ETDEWEB)

    1979-09-28

    TERA's estimates of capital requirements to transport natural gas, crude oil, petroleum products, and coal in the US by 1990 are presented. It is a continuation of a 1978 study (EAPA 5:3946) to perform a similar analysis on 1979 scenarios. Scenarios B, C, and D from the EIA's Mid-range Energy Forecasting Systems, as used in the 1979 Annual Report to Congress (ARC), were provided as a basis for the analysis and represent three alternative futures. Summaries of transportation investment requirements through 1990 are given for Scenarios B, C, and D. Total investment requirements for the three models (pipelines, railroads, waterways) and the three energy commodities (coal, petroleum, petroleum products, natural gas) are estimated to range between $35.3 and $42.7 billion by 1990 depending on the scenario.

  2. Baseline requirements of the proposed action for the Transportation Management Division routing models

    International Nuclear Information System (INIS)

    The potential impacts associated with the transportation of hazardous materials are important to shippers, carriers, and the general public. This is particularly true for shipments of radioactive material. The shippers are primarily concerned with safety, security, efficiency, and equipment requirements. The carriers are concerned with the potential impact that radioactive shipments may have on their operations--particularly if such materials are involved in an accident. The general public has also expressed concerns regarding the safety of transporting radioactive and other hazardous materials through their communities. Because transportation routes are a central concern in hazardous material transport, the prediction of likely routes is the first step toward resolution of these issues. In response to these routing needs, several models have been developed over the past fifteen years at Oak Ridge National Laboratory (ORNL). The HIGHWAY routing model is used to predict routes for truck transportation, the INTERLINE routing model is used to predict both rail and barge routes, and the AIRPORT locator model is used to determine airports with specified criteria near a specific location. As part of the ongoing improvement of the US Department of Energy's (DOE) Environmental Management Transportation Management Division's (EM-261) computer systems and development efforts, a Baseline Requirements Assessment Session on the HIGHWAY, INTERLINE, and AIRPORT models was held at ORNL on April 27, 1994. The purpose of this meeting was to discuss the existing capabilities of the models and data bases and to review enhancements of the models and data bases to expand their usefulness. The results of the Baseline Requirements Assessment Section will be discussed in this report. The discussions pertaining to the different models are contained in separate sections

  3. Baseline requirements of the proposed action for the Transportation Management Division routing models

    Energy Technology Data Exchange (ETDEWEB)

    Johnson, P.E.; Joy, D.S.

    1995-02-01

    The potential impacts associated with the transportation of hazardous materials are important to shippers, carriers, and the general public. This is particularly true for shipments of radioactive material. The shippers are primarily concerned with safety, security, efficiency, and equipment requirements. The carriers are concerned with the potential impact that radioactive shipments may have on their operations--particularly if such materials are involved in an accident. The general public has also expressed concerns regarding the safety of transporting radioactive and other hazardous materials through their communities. Because transportation routes are a central concern in hazardous material transport, the prediction of likely routes is the first step toward resolution of these issues. In response to these routing needs, several models have been developed over the past fifteen years at Oak Ridge National Laboratory (ORNL). The HIGHWAY routing model is used to predict routes for truck transportation, the INTERLINE routing model is used to predict both rail and barge routes, and the AIRPORT locator model is used to determine airports with specified criteria near a specific location. As part of the ongoing improvement of the US Department of Energy`s (DOE) Environmental Management Transportation Management Division`s (EM-261) computer systems and development efforts, a Baseline Requirements Assessment Session on the HIGHWAY, INTERLINE, and AIRPORT models was held at ORNL on April 27, 1994. The purpose of this meeting was to discuss the existing capabilities of the models and data bases and to review enhancements of the models and data bases to expand their usefulness. The results of the Baseline Requirements Assessment Section will be discussed in this report. The discussions pertaining to the different models are contained in separate sections.

  4. The ferric enterobactin transporter Fep is required for persistent Salmonella enterica serovar typhimurium infection.

    Science.gov (United States)

    Nagy, Toni A; Moreland, Sarah M; Andrews-Polymenis, Helene; Detweiler, Corrella S

    2013-11-01

    Most bacterial pathogens require iron to grow and colonize host tissues. The Gram-negative bacterium Salmonella enterica serovar Typhimurium causes a natural systemic infection of mice that models acute and chronic human typhoid fever. S. Typhimurium resides in tissues within cells of the monocyte lineage, which limit pathogen access to iron, a mechanism of nutritional immunity. The primary ferric iron import system encoded by Salmonella is the siderophore ABC transporter FepBDGC. The Fep system has a known role in acute infection, but it is unclear whether ferric iron uptake or the ferric iron binding siderophores enterobactin and salmochelin are required for persistent infection. We defined the role of the Fep iron transporter and siderophores in the replication of Salmonella in macrophages and in mice that develop acute followed by persistent infections. Replication of wild-type and iron transporter mutant Salmonella strains was quantified in cultured macrophages, fecal pellets, and host tissues in mixed- and single-infection experiments. We show that deletion of fepB attenuated Salmonella replication and colonization within macrophages and mice. Additionally, the genes required to produce and transport enterobactin and salmochelin across the outer membrane receptors, fepA and iroN, are needed for colonization of all tissues examined. However, salmochelin appears to be more important than enterobactin in the colonization of the spleen and liver, both sites of dissemination. Thus, the FepBDGC ferric iron transporter and the siderophores enterobactin and salmochelin are required by Salmonella to evade nutritional immunity in macrophages and cause persistent infection in mice.

  5. A method for determining the spent-fuel contribution to transport cask containment requirements

    Energy Technology Data Exchange (ETDEWEB)

    Sanders, T.L.; Seager, K.D. [Sandia National Labs., Albuquerque, NM (United States); Rashid, Y.R.; Barrett, P.R. [ANATECH Research Corp., La Jolla, CA (United States); Malinauskas, A.P. [Oak Ridge National Lab., TN (United States); Einziger, R.E. [Pacific Northwest Lab., Richland, WA (United States); Jordan, H. [EG and G Rocky Flats, Inc., Golden, CO (United States). Rocky Flats Plant; Duffey, T.A.; Sutherland, S.H. [APTEK, Inc., Colorado Springs, CO (United States); Reardon, P.C. [GRAM, Inc., Albuquerque, NM (United States)

    1992-11-01

    This report examines containment requirements for spent-fuel transport containers that are transported under normal and hypothetical accident conditions. A methodology is described that estimates the probability of rod failure and the quantity of radioactive material released from breached rods. This methodology characterizes the dynamic environment of the cask and its contents and deterministically models the peak stresses that are induced in spent-fuel cladding by the mechanical and thermal dynamic environments. The peak stresses are evaluated in relation to probabilistic failure criteria for generated or preexisting ductile tearing and material fractures at cracks partially through the wall in fuel rods. Activity concentrations in the cask cavity are predicted from estimates of the fraction of gases, volatiles, and fuel fines that are released when the rod cladding is breached. Containment requirements based on the source term are calculated in terms of maximum permissible volumetric leak rates from the cask. Calculations are included for representative cask designs.

  6. A method for determining the spent-fuel contribution to transport cask containment requirements

    International Nuclear Information System (INIS)

    This report examines containment requirements for spent-fuel transport containers that are transported under normal and hypothetical accident conditions. A methodology is described that estimates the probability of rod failure and the quantity of radioactive material released from breached rods. This methodology characterizes the dynamic environment of the cask and its contents and deterministically models the peak stresses that are induced in spent-fuel cladding by the mechanical and thermal dynamic environments. The peak stresses are evaluated in relation to probabilistic failure criteria for generated or preexisting ductile tearing and material fractures at cracks partially through the wall in fuel rods. Activity concentrations in the cask cavity are predicted from estimates of the fraction of gases, volatiles, and fuel fines that are released when the rod cladding is breached. Containment requirements based on the source term are calculated in terms of maximum permissible volumetric leak rates from the cask. Calculations are included for representative cask designs

  7. Review of Transporter-Related Postmarketing Requirement or Postmarketing Commitment Studies.

    Science.gov (United States)

    Fan, Ying; Sun, Bo; Agarwal, Sheetal; Zhang, Lei

    2016-07-01

    The objectives of this report are to summarize the content and status of transporter-related postmarketing requirement (PMR)/postmarketing commitment (PMC) studies in new drug applications (NDAs) approved by the U.S. Food and Drug Administration (FDA) and to discuss the reasons for requesting such studies and the impact of PMR/PMC study results on labeling to guide the optimal use of the drugs. Multiple data sources were searched to collect information on transporter-related PMR/PMC studies between January 1999 and May 2015. A total of 40 transporter-related PMR/PMC study requests were issued for 35 NDAs. Among these PMR/PMC studies, 27 requested studies related to P-glycoprotein. As of May 31, 2015, 34 transporter-related PMR/PMC studies (85%) are considered "fulfilled" (per the FDA's PMR/PMC website), and 22 (65%) resulted in labeling updates. The majority of the PMR/PMC studies are for drugs in the therapeutic areas of anti-infectives, oncology, and neurology. The results from PMR/PMC studies are important for dosing optimization and are often included in the updated labeling. Because a significant lag time is anticipated between drug approval and PMR/PMC fulfillment, NDA applicants are encouraged to include transporter-related assessments in clinical drug development programs for drug products. PMID:27385175

  8. Negative membrane curvature catalyzes nucleation of endosomal sorting complex required for transport (ESCRT)-III assembly

    OpenAIRE

    Lee, IH; Kai, H; Carlson, LA; Groves, JT; Hurley, JH

    2015-01-01

    The endosomal sorting complexes required for transport (ESCRT) machinery functions in HIV-1 budding, cytokinesis, multivesicular body biogenesis, and other pathways, in the course of which it interacts with concave membrane necks and bud rims. To test the role of membrane shape in regulating ESCRT assembly, we nanofabricated templates for invaginated supported lipid bilayers. The assembly of the core ESCRT-III subunit CHMP4B/Snf7 is preferentially nucleated in the resulting 100-nm-deep membra...

  9. Kymographic Analysis of Transport in an Individual Neuronal Sensory Cilium in Caenorhabditis elegans.

    Science.gov (United States)

    O'Hagan, Robert; Barr, Maureen M

    2016-01-01

    Intraflagellar Transport (IFT) is driven by molecular motors that travel upon microtubule-based ciliary axonemes. In the single-celled alga Chlamydomonas reinhardtii, movement of a single anterograde IFT motor, heterotrimeric kinesin-II, is required to generate two identical motile flagella. The function of this canonical anterograde IFT motor is conserved among all eukaryotes, yet multicellular organisms can generate cilia of diverse structures and functions, ranging from simple threadlike non-motile primary cilia to the elaborate cilia that make up rod and cone photoreceptors in the retina. An emerging theme is that additional molecular motors modulate the canonical IFT machinery to give rise to differing ciliary morphologies. Therefore, a complete understanding of the trafficking of ciliary receptors, as well as the biogenesis, maintenance, specialization, and function of cilia, requires the characterization of motor molecules.Here, we describe in detail our method for measuring the motility of proteins in cilia or dendrites of C. elegans male-specific CEM ciliated sensory neurons using time-lapse microscopy and kymography of green fluorescent protein (GFP)-tagged motors, receptors, and cargos. We describe, as a specific example, OSM-3::GFP puncta moving in cilia, but also include (Fig. 1) with settings that have worked well for us measuring movement of heterotrimeric kinesin-II, IFT particles, and the polycystin TRP channel PKD-2. PMID:27514919

  10. Dynamics of oligodendrocyte responses to anterograde axonal (Wallerian) and terminal degeneration in normal and TNF-transgenic mice

    DEFF Research Database (Denmark)

    Drøjdahl, Nina; Fenger, Christina; Nielsen, Helle H;

    2004-01-01

    larger levels in the TNF-transgenics. At 5 days after axonal transection, numbers of oligodendrocytes and myelin basic protein (MBP) mRNA expression in the denervated dentate gyrus in TNF-transgenic mice had increased to the same extent as in nontransgenic littermates. At this time, transgenics showed......The inflammatory cytokine tumour necrosis factor (TNF) can both induce oligodendrocyte and myelin pathology and promote proliferation of oligodendrocyte progenitor cells and remyelination. We have compared the response of the oligodendrocyte lineage to anterograde axonal (Wallerian) and terminal...

  11. Alternative transportation fuels: Infrastructure requirements and environmental impacts for ethanol and hydrogen

    Science.gov (United States)

    Wakeley, Heather L.

    Alternative fuels could replace a significant portion of the 140 billion gallons of annual US gasoline use. Considerable attention is being paid to processes and technologies for producing alternative fuels, but an enormous investment in new infrastructure will be needed to have substantial impact on the demand for petroleum. The economics of production, distribution, and use, along with environmental impacts of these fuels, will determine the success or failure of a transition away from US petroleum dependence. This dissertation evaluates infrastructure requirements for ethanol and hydrogen as alternative fuels. It begins with an economic case study for ethanol and hydrogen in Iowa. A large-scale linear optimization model is developed to estimate average transportation distances and costs for nationwide ethanol production and distribution systems. Environmental impacts of transportation in the ethanol life cycle are calculated using the Economic Input-Output Life Cycle Assessment (EIO-LCA) model. An EIO-LCA Hybrid method is developed to evaluate impacts of future fuel production technologies. This method is used to estimate emissions for hydrogen production and distribution pathways. Results from the ethanol analyses indicate that the ethanol transportation cost component is significant and is the most variable. Costs for ethanol sold in the Midwest, near primary production centers, are estimated to be comparable to or lower than gasoline costs. Along with a wide range of transportation costs, environmental impacts for ethanol range over three orders of magnitude, depending on the transport required. As a result, intensive ethanol use should be encouraged near ethanol production areas. Fossil fuels are likely to remain the primary feedstock sources for hydrogen production in the near- and mid-term. Costs and environmental impacts of hydrogen produced from natural gas and transported by pipeline are comparable to gasoline. However, capital costs are prohibitive and

  12. Competence and Capacity-Building Requirements in Transport and Logistics Market

    Directory of Open Access Journals (Sweden)

    Bazaras Darius

    2016-03-01

    Full Text Available The article analyses theoretical aspects of the qualitative assessment of logistics and transport specialists’ potential, as well as provides research results on competence and capacity-building process in preparation of logistics and transport specialists. The results enabled to identify problematic areas of graduate integration in the labour market and foresee the possible requirements for professional development. In some cases, internal motivation is related to problematic points or negative view from the environment, as well as poor understanding of knowledge benefits in the person’s practical life. Training and education is objectively at the meeting point between research, technological and organisational systems. In such conditions the objective of the education on logistics is to offer the customers the best possible service in the field of the transfer of knowledge in harmony with the trend in logistics programme development. The main problems and perspectives of logistics education and training process are discussed in this article.

  13. Multidrug Resistance–like Genes of Arabidopsis Required for Auxin Transport and Auxin-Mediated Development

    Science.gov (United States)

    Noh, Bosl; Murphy, Angus S.; Spalding, Edgar P.

    2001-01-01

    Arabidopsis possesses several genes related to the multidrug resistance (MDR) genes of animals, one of which, AtMDR1, was shown to be induced by the hormone auxin. Plants having mutations in AtMDR1 or its closest relative, AtPGP1, were isolated by a reverse genetic strategy. Auxin transport activity was greatly impaired in atmdr1 and atmdr1 atpgp1 double mutant plants. Epinastic cotyledons and reduced apical dominance were mutant phenotypes consistent with the disrupted basipetal flow of auxin. The auxin transport inhibitor 1-naphthylphthalamic acid was shown to bind tightly and specifically to AtMDR1 and AtPGP1 proteins. The results indicate that these two MDR-like genes of Arabidopsis encode 1-naphthylphthalamic acid binding proteins that are required for normal auxin distribution and auxin-mediated development. PMID:11701880

  14. Active diffusion and microtubule-based transport oppose myosin forces to position organelles in cells

    Science.gov (United States)

    Lin, Congping; Schuster, Martin; Guimaraes, Sofia Cunha; Ashwin, Peter; Schrader, Michael; Metz, Jeremy; Hacker, Christian; Gurr, Sarah Jane; Steinberg, Gero

    2016-06-01

    Even distribution of peroxisomes (POs) and lipid droplets (LDs) is critical to their role in lipid and reactive oxygen species homeostasis. How even distribution is achieved remains elusive, but diffusive motion and directed motility may play a role. Here we show that in the fungus Ustilago maydis ~95% of POs and LDs undergo diffusive motions. These movements require ATP and involve bidirectional early endosome motility, indicating that microtubule-associated membrane trafficking enhances diffusion of organelles. When early endosome transport is abolished, POs and LDs drift slowly towards the growing cell end. This pole-ward drift is facilitated by anterograde delivery of secretory cargo to the cell tip by myosin-5. Modelling reveals that microtubule-based directed transport and active diffusion support distribution, mobility and mixing of POs. In mammalian COS-7 cells, microtubules and F-actin also counteract each other to distribute POs. This highlights the importance of opposing cytoskeletal forces in organelle positioning in eukaryotes.

  15. Active diffusion and microtubule-based transport oppose myosin forces to position organelles in cells

    Science.gov (United States)

    Lin, Congping; Schuster, Martin; Guimaraes, Sofia Cunha; Ashwin, Peter; Schrader, Michael; Metz, Jeremy; Hacker, Christian; Gurr, Sarah Jane; Steinberg, Gero

    2016-01-01

    Even distribution of peroxisomes (POs) and lipid droplets (LDs) is critical to their role in lipid and reactive oxygen species homeostasis. How even distribution is achieved remains elusive, but diffusive motion and directed motility may play a role. Here we show that in the fungus Ustilago maydis ∼95% of POs and LDs undergo diffusive motions. These movements require ATP and involve bidirectional early endosome motility, indicating that microtubule-associated membrane trafficking enhances diffusion of organelles. When early endosome transport is abolished, POs and LDs drift slowly towards the growing cell end. This pole-ward drift is facilitated by anterograde delivery of secretory cargo to the cell tip by myosin-5. Modelling reveals that microtubule-based directed transport and active diffusion support distribution, mobility and mixing of POs. In mammalian COS-7 cells, microtubules and F-actin also counteract each other to distribute POs. This highlights the importance of opposing cytoskeletal forces in organelle positioning in eukaryotes. PMID:27251117

  16. Active diffusion and microtubule-based transport oppose myosin forces to position organelles in cells.

    Science.gov (United States)

    Lin, Congping; Schuster, Martin; Guimaraes, Sofia Cunha; Ashwin, Peter; Schrader, Michael; Metz, Jeremy; Hacker, Christian; Gurr, Sarah Jane; Steinberg, Gero

    2016-01-01

    Even distribution of peroxisomes (POs) and lipid droplets (LDs) is critical to their role in lipid and reactive oxygen species homeostasis. How even distribution is achieved remains elusive, but diffusive motion and directed motility may play a role. Here we show that in the fungus Ustilago maydis ∼95% of POs and LDs undergo diffusive motions. These movements require ATP and involve bidirectional early endosome motility, indicating that microtubule-associated membrane trafficking enhances diffusion of organelles. When early endosome transport is abolished, POs and LDs drift slowly towards the growing cell end. This pole-ward drift is facilitated by anterograde delivery of secretory cargo to the cell tip by myosin-5. Modelling reveals that microtubule-based directed transport and active diffusion support distribution, mobility and mixing of POs. In mammalian COS-7 cells, microtubules and F-actin also counteract each other to distribute POs. This highlights the importance of opposing cytoskeletal forces in organelle positioning in eukaryotes. PMID:27251117

  17. Assessment and Requirements of Nuclear Reaction Databases for GCR Transport in the Atmosphere and Structures

    Science.gov (United States)

    Cucinotta, F. A.; Wilson, J. W.; Shinn, J. L.; Tripathi, R. K.

    1998-01-01

    The transport properties of galactic cosmic rays (GCR) in the atmosphere, material structures, and human body (self-shielding) am of interest in risk assessment for supersonic and subsonic aircraft and for space travel in low-Earth orbit and on interplanetary missions. Nuclear reactions, such as knockout and fragmentation, present large modifications of particle type and energies of the galactic cosmic rays in penetrating materials. We make an assessment of the current nuclear reaction models and improvements in these model for developing required transport code data bases. A new fragmentation data base (QMSFRG) based on microscopic models is compared to the NUCFRG2 model and implications for shield assessment made using the HZETRN radiation transport code. For deep penetration problems, the build-up of light particles, such as nucleons, light clusters and mesons from nuclear reactions in conjunction with the absorption of the heavy ions, leads to the dominance of the charge Z = 0, 1, and 2 hadrons in the exposures at large penetration depths. Light particles are produced through nuclear or cluster knockout and in evaporation events with characteristically distinct spectra which play unique roles in the build-up of secondary radiation's in shielding. We describe models of light particle production in nucleon and heavy ion induced reactions and make an assessment of the importance of light particle multiplicity and spectral parameters in these exposures.

  18. Use of simple transport equations to estimate waste package performance requirements

    International Nuclear Information System (INIS)

    A method of developing waste package performance requirements for specific nuclides is described. The method is based on: Federal regulations concerning permissible concentrations in solution at the point of discharge to the accessible environment; a simple and conservative transport model; baseline and potential worst-case release scenarios. Use of the transport model enables calculation of maximum permissible release rates within a repository in basalt for each of the scenarios. The maximum permissible release rates correspond to performance requirements for the engineered barrier system. The repository was assumed to be constructed in a basalt layer. For the cases considered, including a well drilled into an aquifer 1750 m from the repository center, little significant advantage is obtained from a 1000-yr as opposed to a 100-yr waste package. A 1000-yr waste package is of importance only for nuclides with half-lives much less than 100 yr which travel to the accessible environment in much less than 1000 yr. Such short travel times are extremely unlikely for a mined repository. Among the actinides, the most stringent maximum permissible release rates are for 236U and 234U. A simple solubility calculation suggests, however, that these performance requirements can be readily met by the engineered barrier system. Under the reducing conditions likely to occur in a repository located in basalt, uranium would be sufficiently insoluble that no solution could contain more than about 0.01% of the maximum permissible concentration at saturation. The performance requirements derived from the one-dimensional modeling approach are conservative by at least one to two orders of magnitude. More quantitative three-dimensional modeling at specific sites should enable relaxation of the performance criteria derived in this study. 12 references, 8 figures, 8 tables

  19. Chlamydomonas IFT70/CrDYF-1 is a core component of IFT particle complex B and is required for flagellar assembly.

    Science.gov (United States)

    Fan, Zhen-Chuan; Behal, Robert H; Geimer, Stefan; Wang, Zhaohui; Williamson, Shana M; Zhang, Haili; Cole, Douglas G; Qin, Hongmin

    2010-08-01

    DYF-1 is a highly conserved protein essential for ciliogenesis in several model organisms. In Caenorhabditis elegans, DYF-1 serves as an essential activator for an anterograde motor OSM-3 of intraflagellar transport (IFT), the ciliogenesis-required motility that mediates the transport of flagellar precursors and removal of turnover products. In zebrafish and Tetrahymena DYF-1 influences the cilia tubulin posttranslational modification and may have more ubiquitous function in ciliogenesis than OSM-3. Here we address how DYF-1 biochemically interacts with the IFT machinery by using the model organism Chlamydomonas reinhardtii, in which the anterograde IFT does not depend on OSM-3. Our results show that this protein is a stoichiometric component of the IFT particle complex B and interacts directly with complex B subunit IFT46. In concurrence with the established IFT protein nomenclature, DYF-1 is also named IFT70 after the apparent size of the protein. IFT70/CrDYF-1 is essential for the function of IFT in building the flagellum because the flagella of IFT70/CrDYF-1-depleted cells were greatly shortened. Together, these results demonstrate that IFT70/CrDYF-1 is a canonical subunit of IFT particle complex B and strongly support the hypothesis that the IFT machinery has species- and tissue-specific variations with functional ramifications. PMID:20534810

  20. Patterns of Direct Projections from the Hippocampus to the Medial Septum-Diagonal Band Complex : Anterograde Tracing with Phaseolus vulgaris Leucoagglutinin Combined with Immunohistochemistry of Choline Acetyltransferase

    NARCIS (Netherlands)

    Gaykema, R.P.A.; Kuil, J. van der; Hersh, L.B.; Luiten, P.G.M.

    1991-01-01

    The projections from the Ammon's horn to the cholinergic cell groups in the medial septal and diagonal band nuclei were investigated with anterograde tracing of Phaseolus vulgaris leucoagglutinin combined with immunocytochemical detection of choline acetyltransferase, in the rat. Tracer injections w

  1. Transition fibre protein FBF1 is required for the ciliary entry of assembled intraflagellar transport complexes.

    Science.gov (United States)

    Wei, Qing; Xu, Qingwen; Zhang, Yuxia; Li, Yujie; Zhang, Qing; Hu, Zeng; Harris, Peter C; Torres, Vicente E; Ling, Kun; Hu, Jinghua

    2013-01-01

    Sensory organelle cilia have critical roles in mammalian embryonic development and tissue homeostasis. Intraflagellar transport (IFT) machinery is required for the assembly and maintenance of cilia. Yet, how this large complex passes through the size-dependent barrier at the ciliary base remains enigmatic. Here we report that FBF1, a highly conserved transition fibre protein, is required for the ciliary import of assembled IFT particles at the ciliary base. We cloned dyf-19, the Caenorhabditis elegans homologue of human FBF1, in a whole-genome screen for ciliogenesis mutants. DYF-19 localizes specifically to transition fibres and interacts directly with the IFT-B component DYF-11/IFT54. Although not a structural component of transition fibres, DYF-19 is required for the transit of assembled IFT particles through the ciliary base. Furthermore, we found that human FBF1 shares conserved localization and function with its worm counterpart. We conclude that FBF1 is a key functional transition fibre component that actively facilitates the ciliary entry of assembled IFT machinery. PMID:24231678

  2. Automated container transportation using self-guided vehicles: Fernald site requirements

    Energy Technology Data Exchange (ETDEWEB)

    Hazen, F.B.

    1993-09-01

    A new opportunity to improve the safety and efficiency of environmental restoration operations, using robotics has emerged from advances in industry, academia, and government labs. Self-Guided Vehicles (SGV`s) have recently been developed in industry and early systems have already demonstrated much, though not all, of the functionality necessary to support driverless transportation of waste within and between processing facilities. Improved materials databases are being developed by at least two DOE remediation sites, the Fernald Environmental Management Project (FEME) in the State of Ohio and the Hanford Complex in the State of Washington. SGV`s can be developed that take advantage of the information in these databases and yield improved dispatch, waste tracking, report and shipment documentation. In addition, they will reduce the radiation hazard to workers and the risk of damaging containers through accidental collision. In this document, features of remediation sites that dictate the design of both the individual SGV`s and the collective system of SGV`s are presented, through the example of the site requirements at Fernald. Some concepts borrowed from the world of manufacturing are explained and then used to develop an integrated, holistic view of the remediation site as a pseudo-factory. Transportation methods at Fernald and anticipated growth in transport demand are analyzed. The new site-wide database under development at Fernald is presented so that advantageous and synergistic links between SGV`s and information systems can be analyzed. Details of the SGV development proposed are submitted, and some results of a recently completed state of the art survey for SGV use in this application are also presented.

  3. Automated container transportation using self-guided vehicles: Fernald site requirements

    International Nuclear Information System (INIS)

    A new opportunity to improve the safety and efficiency of environmental restoration operations, using robotics has emerged from advances in industry, academia, and government labs. Self-Guided Vehicles (SGV's) have recently been developed in industry and early systems have already demonstrated much, though not all, of the functionality necessary to support driverless transportation of waste within and between processing facilities. Improved materials databases are being developed by at least two DOE remediation sites, the Fernald Environmental Management Project (FEME) in the State of Ohio and the Hanford Complex in the State of Washington. SGV's can be developed that take advantage of the information in these databases and yield improved dispatch, waste tracking, report and shipment documentation. In addition, they will reduce the radiation hazard to workers and the risk of damaging containers through accidental collision. In this document, features of remediation sites that dictate the design of both the individual SGV's and the collective system of SGV's are presented, through the example of the site requirements at Fernald. Some concepts borrowed from the world of manufacturing are explained and then used to develop an integrated, holistic view of the remediation site as a pseudo-factory. Transportation methods at Fernald and anticipated growth in transport demand are analyzed. The new site-wide database under development at Fernald is presented so that advantageous and synergistic links between SGV's and information systems can be analyzed. Details of the SGV development proposed are submitted, and some results of a recently completed state of the art survey for SGV use in this application are also presented

  4. Functional coordination of intraflagellar transport motors.

    Science.gov (United States)

    Ou, Guangshuo; Blacque, Oliver E; Snow, Joshua J; Leroux, Michel R; Scholey, Jonathan M

    2005-07-28

    Cilia have diverse roles in motility and sensory reception, and defects in cilia function contribute to ciliary diseases such as Bardet-Biedl syndrome (BBS). Intraflagellar transport (IFT) motors assemble and maintain cilia by transporting ciliary precursors, bound to protein complexes called IFT particles, from the base of the cilium to their site of incorporation at the distal tip. In Caenorhabditis elegans, this is accomplished by two IFT motors, kinesin-II and osmotic avoidance defective (OSM)-3 kinesin, which cooperate to form two sequential anterograde IFT pathways that build distinct parts of cilia. By observing the movement of fluorescent IFT motors and IFT particles along the cilia of numerous ciliary mutants, we identified three genes whose protein products mediate the functional coordination of these motors. The BBS proteins BBS-7 and BBS-8 are required to stabilize complexes of IFT particles containing both of the IFT motors, because IFT particles in bbs-7 and bbs-8 mutants break down into two subcomplexes, IFT-A and IFT-B, which are moved separately by kinesin-II and OSM-3 kinesin, respectively. A conserved ciliary protein, DYF-1, is specifically required for OSM-3 kinesin to dock onto and move IFT particles, because OSM-3 kinesin is inactive and intact IFT particles are moved by kinesin-II alone in dyf-1 mutants. These findings implicate BBS ciliary disease proteins and an OSM-3 kinesin activator in the formation of two IFT pathways that build functional cilia. PMID:16049494

  5. Requirements for a systems-based research and development management process in transport infrastructure engineering

    Directory of Open Access Journals (Sweden)

    Rust, Frederik Christoffel

    2015-05-01

    Full Text Available The management of research and development (R&D in the transport infrastructure field is complex due to the multidisciplinary nature of the work. The literature shows that linear R&D models that progress from idea through to consumer product are not suitable for the management of such multi-disciplinary projects. This study focuses on determining the key characteristics required for a systems-based approach to the management of R&D projects. The information and data was compiled from literature reviews, interviews, and an e-mail survey with responses from 42 significant international R&D programmes. The findings confirmed the need for a systems-based approach to R&D management. The study formulated twelve principles or tenets for a new, systems-based approach.

  6. Schedules of requirements for the transport of specified types of radioactive material consignments (as amended 1990)

    International Nuclear Information System (INIS)

    This publication replaces the previous issue of Safety Series No. 80. It includes the changes to Safety Series No. 80 contained in Supplement 1988 to the Regulations for the Safe Transport of Radioactive Material as well as some modifications adopted by the Review Panel meeting which convened in Vienna, from 10 to 14 July 1989. The old IAEA Safety Series style is maintained for the convenience of the user, although the old style has now been superseded by a new one, affecting the structure, the format and the cover of the Safety Series. It should be noted, however, that subsequent editions of the Regulations and the Schedules of Requirements will be published in the new style. Tabs

  7. Systemic or intra-amygdala infusion of an endocannabinoid CB1 receptor antagonist AM251 blocked propofol-induced anterograde amnesia.

    Science.gov (United States)

    Ren, Y; Wang, J; Xu, P B; Xu, Y J; Miao, C H

    2015-01-01

    Propofol is well-known for its anterograde amnesic actions. However, a recent experiment showed that propofol can also produce retrograde memory enhancement effects via an interaction with the endocannabinoid CB1 system. Therefore, the authors hypothesized that the regulating effect of propofol on the endocannabinoid CB1 system might also decrease the anterograde amnesic effect of propofol under some conditions, which might be a risk factor for intraoperative awareness. Since, the basolateral amygdala (BLA) has been confirmed to mediate propofol-induced anterograde amnesia and the BLA contains a high concentration of CB1 receptors, the authors investigated whether and how the endocannabinoid system, particularly the CB1 receptor within BLA, influences propofol-induced anterograde amnesia. Male Sprague-Dawley rats trained with inhibitory avoidance (IA) were systematically pre-trained using a memory-impairing dose of propofol (25 mg/kg). Before propofol administration, rats received an intraperitoneal injection of a CB1 receptor antagonist AM251 (1 mg/kg or 2 mg/kg) or a bilateral intra-BLA injection of AM251 (0.6 ng or 6 ng per 0.5 μl). Twenty-four hours after IA training, the IA retention latency was tested. It was found that systemic or intra-BLA injection of a non-regulating dose of AM251 (2 mg/kg or 6 ng per 0.5 μl, respectively) blocked the memory-impairing effect of propofol. These results indicate that the anterograde amnesic effect of propofol is mediated, in part, by activation of the CB1 cannabinoid receptors in the BLA.

  8. Schedules of requirements for the transport of specified types of radioactive material consignments

    International Nuclear Information System (INIS)

    These Schedules have been prepared as a companion document to the 1985 Edition of the Transport Regulations (Safety Series No. 6). As presented here, the Schedules also reflect the corrections and changes implemented by the 1986 Supplement to the Regulations for the Safe Transport of Radioactive Material. Direct cross-referencing to the paragraphs, tables and figures in the Regulations is made, and the use of both the International System of Units (SI) and the formerly accepted, traditional units is continued. In many cases, due to rounding off the numbers, the two values for a given parameter for the two units may differ somewhat. To move toward international use of SI units and to avoid inconsistencies, the values for the SI units are controlling in all cases. These Schedules are provided only as an aid to users of the Regulations and the provisions of the Regulations take precedence over the Schedules. These Schedules also serve as a generic model of the Schedules which are part of modal international regulatory documents, such as the International Maritime Dangerous Goods (IMDG) Code of the International Maritime Organization (IMO), the European Agreement Concerning the International Carriage of Dangerous Goods by Road (ADR) and the International Convention Concerning the Carriage of Dangerous Goods by Rail (CIM/RID). For regulatory purposes, reference shall be made to the detailed provisions of the Regulations specified in Safety Series No. 6. The Schedules do not reproduce the provisions of the Regulations in detail nor do they contain any additional requirements. They merely provide a summary of the main provisions for each specified type of radioactive material, references being provided to the relevant detailed provisions of the Regulations to enable these to be consulted where necessary. A table summarizing package, shipment approval, and notification requirements of the Regulations is provided

  9. The Arabidopsis Golgi-localized GDP-L-fucose transporter is required for plant development

    OpenAIRE

    Rautengarten, Carsten; Ebert, Berit; Liu, Lifeng; Stonebloom, Solomon; Smith-Moritz, Andreia M.; Pauly, Markus; Orellana, Ariel; Scheller, Henrik Vibe; Heazlewood, Joshua L.

    2016-01-01

    Nucleotide sugar transport across Golgi membranes is essential for the luminal biosynthesis of glycan structures. Here we identify GDP-fucose transporter 1 (GFT1), an Arabidopsis nucleotide sugar transporter that translocates GDP-L-fucose into the Golgi lumen. Using proteo-liposome-based transport assays, we show that GFT preferentially transports GDP-L-fucose over other nucleotide sugars in vitro, while GFT1-silenced plants are almost devoid of L-fucose in cell wall-derived xyloglucan and rh...

  10. WD60/FAP163 is a dynein intermediate chain required for retrograde intraflagellar transport in cilia.

    Science.gov (United States)

    Patel-King, Ramila S; Gilberti, Renée M; Hom, Erik F Y; King, Stephen M

    2013-09-01

    Retrograde intraflagellar transport (IFT) is required for assembly of cilia. We identify a Chlamydomonas flagellar protein (flagellar-associated protein 163 [FAP163]) as being closely related to the D1bIC(FAP133) intermediate chain (IC) of the dynein that powers this movement. Biochemical analysis revealed that FAP163 is present in the flagellar matrix and is actively trafficked by IFT. Furthermore, FAP163 copurified with D1bIC(FAP133) and the LC8 dynein light chain, indicating that it is an integral component of the retrograde IFT dynein. To assess the functional role of FAP163, we generated an RNA interference knockdown of the orthologous protein (WD60) in planaria. The Smed-wd60(RNAi) animals had a severe ciliary assembly defect that dramatically compromised whole-organism motility. Most cilia were present as short stubs that had accumulated large quantities of IFT particle-like material between the doublet microtubules and the membrane. The few remaining approximately full-length cilia had a chaotic beat with a frequency reduced from 24 to ∼10 Hz. Thus WD60/FAP163 is a dynein IC that is absolutely required for retrograde IFT and ciliary assembly. PMID:23864713

  11. WD60/FAP163 is a dynein intermediate chain required for retrograde intraflagellar transport in cilia

    Science.gov (United States)

    Patel-King, Ramila S.; Gilberti, Renée M.; Hom, Erik F. Y.; King, Stephen M.

    2013-01-01

    Retrograde intraflagellar transport (IFT) is required for assembly of cilia. We identify a Chlamydomonas flagellar protein (flagellar-associated protein 163 [FAP163]) as being closely related to the D1bIC(FAP133) intermediate chain (IC) of the dynein that powers this movement. Biochemical analysis revealed that FAP163 is present in the flagellar matrix and is actively trafficked by IFT. Furthermore, FAP163 copurified with D1bIC(FAP133) and the LC8 dynein light chain, indicating that it is an integral component of the retrograde IFT dynein. To assess the functional role of FAP163, we generated an RNA interference knockdown of the orthologous protein (WD60) in planaria. The Smed-wd60(RNAi) animals had a severe ciliary assembly defect that dramatically compromised whole-organism motility. Most cilia were present as short stubs that had accumulated large quantities of IFT particle–like material between the doublet microtubules and the membrane. The few remaining approximately full-length cilia had a chaotic beat with a frequency reduced from 24 to ∼10 Hz. Thus WD60/FAP163 is a dynein IC that is absolutely required for retrograde IFT and ciliary assembly. PMID:23864713

  12. 30 CFR 206.177 - What general requirements regarding transportation allowances apply to me?

    Science.gov (United States)

    2010-07-01

    ... transportation allowances apply to me? 206.177 Section 206.177 Mineral Resources MINERALS MANAGEMENT SERVICE, DEPARTMENT OF THE INTERIOR MINERALS REVENUE MANAGEMENT PRODUCT VALUATION Indian Gas Transportation Allowances..., transportation from the lease to a gas processing plant off the lease, unit, or communitized area and from...

  13. Requirements for traffic assignment models for strategic transport planning: a critical assessment

    NARCIS (Netherlands)

    Bliemer, M.; Raadsen, M.; De Romph, E.; Smits, E.

    2013-01-01

    Transport planning models are used all over the world to assist in the decision making regarding investments in infrastructure and transport services. Traffic assignment is one of the key components of transport models, which relate travel demand to infrastructure supply, by simulating (future) rout

  14. The Copper Transporter 1 (CTR1) is Required to Maintain the Stability of Copper Transporter 2 (CTR2)

    Science.gov (United States)

    Tsai, Cheng-Yu; Liebig, Janika K.; Tsigelny, Igor F.; Howell, Stephen B.

    2015-01-01

    Mammalian cells have two influx Cu transporters that form trimers in membranes. CTR1 is the high affinity transporter that resides largely in the plasma membrane, and CTR2 is the low affinity transporter that is primarily associated with vesicular structures inside the cell. The major differences between CTR1 and CTR2 are that CTR1 contains a HIS/MET-rich domain N-terminal of the METS that participate in the first two stacked rings that form the pore, and a longer C-terminal tail that includes a Cu binding HIS-CYS-HIS (HCH) motif right at the end. It has been reported that CTR1 and CTR2 are physically associated with each other in the cell. We used the CRISPR-Cas9 technology to knock out either CTR1 or CTR2 in fully malignant HEK293T and OVCAR8 human ovarian cancer cells to investigate the interaction of CTR1 and CTR2. We report here that the level of CTR2 protein is markedly decreased in CTR1 knockout clones while the CTR2 transcript level remains unchanged. CTR2 was found to be highly ubiquitinated in the CTR1 knock out cells, and inhibition of the proteosome prevented the degradation of CTR2 when CTR1 was not present while inhibition of autophagy had no effect. Re-expression of CTR1 rescued CTR2 from degradation in the CTR1 knockout cells. We conclude that CTR1 is essential to maintain the stability of CTR2 and that in the absence of CTR1 CTR2 is degraded by the proteosome. This reinforces the concept that the functions of CTR1 and CTR2 are inter-dependent within the Cu homeostasis system. PMID:26205368

  15. The copper transporter 1 (CTR1) is required to maintain the stability of copper transporter 2 (CTR2).

    Science.gov (United States)

    Tsai, Cheng-Yu; Liebig, Janika K; Tsigelny, Igor F; Howell, Stephen B

    2015-11-01

    Mammalian cells have two influx Cu transporters that form trimers in membranes. CTR1 is the high affinity transporter that resides largely in the plasma membrane, and CTR2 is the low affinity transporter that is primarily associated with vesicular structures inside the cell. The major differences between CTR1 and CTR2 are that CTR1 contains a HIS/MET-rich domain N-terminal of the METS that participate in the first two stacked rings that form the pore, and a longer C-terminal tail that includes a Cu binding HIS-CYS-HIS (HCH) motif right at the end. It has been reported that CTR1 and CTR2 are physically associated with each other in the cell. We used the CRISPR-Cas9 technology to knock out either CTR1 or CTR2 in fully malignant HEK293T and OVCAR8 human ovarian cancer cells to investigate the interaction of CTR1 and CTR2. We report here that the level of CTR2 protein is markedly decreased in CTR1 knockout clones while the CTR2 transcript level remains unchanged. CTR2 was found to be highly ubiquitinated in the CTR1 knock out cells, and inhibition of the proteasome prevented the degradation of CTR2 when CTR1 was not present while inhibition of autophagy had no effect. Re-expression of CTR1 rescued CTR2 from degradation in the CTR1 knockout cells. We conclude that CTR1 is essential to maintain the stability of CTR2 and that in the absence of CTR1 CTR2 is degraded by the proteasome. This reinforces the concept that the functions of CTR1 and CTR2 are inter-dependent within the Cu homeostasis system.

  16. 9 CFR 11.40 - Prohibitions and requirements concerning persons involved in transportation of certain horses.

    Science.gov (United States)

    2010-01-01

    ... concerning persons involved in transportation of certain horses. 11.40 Section 11.40 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE ANIMAL WELFARE HORSE... certain horses. (a) Each person who ships, transports, or otherwise moves, or delivers or receives...

  17. Requirements Elicitation of Passengers With Reduced Mobility for the Design of High Quality, Accessible and Inclusive Public Transport Services

    Directory of Open Access Journals (Sweden)

    Starzyńska Beata

    2015-09-01

    Full Text Available In this article the authors present the methodology adopted and the results obtained in the first stage of the research encompassing focus group interviews (FGI about the needs of public transport users in a selected city (Poznań. The elicitation and assessment of the requirements were carried out for three groups of people with disabilities using public transport in the city of Poznań: blind and partially sighted people, deaf and hearing-impaired people, as well as people with locomotor dysfunctions. A study carried out on the basis of a scenario especially designed for the FGI purpose has made it possible to identify barriers for people with disabilities and, consequently, to formulate their pre-trip,on-trip and post-trip requirements when it comes to urban public transport services. The results will be used to construct a questionnaire to be used further on in the project.

  18. TTC26/DYF13 is an intraflagellar transport protein required for transport of motility-related proteins into flagella

    Science.gov (United States)

    Ishikawa, Hiroaki; Ide, Takahiro; Yagi, Toshiki; Jiang, Xue; Hirono, Masafumi; Sasaki, Hiroyuki; Yanagisawa, Haruaki; Wemmer, Kimberly A; Stainier, Didier YR; Qin, Hongmin; Kamiya, Ritsu; Marshall, Wallace F

    2014-01-01

    Cilia/flagella are assembled and maintained by the process of intraflagellar transport (IFT), a highly conserved mechanism involving more than 20 IFT proteins. However, the functions of individual IFT proteins are mostly unclear. To help address this issue, we focused on a putative IFT protein TTC26/DYF13. Using live imaging and biochemical approaches we show that TTC26/DYF13 is an IFT complex B protein in mammalian cells and Chlamydomonas reinhardtii. Knockdown of TTC26/DYF13 in zebrafish embryos or mutation of TTC26/DYF13 in C. reinhardtii, produced short cilia with abnormal motility. Surprisingly, IFT particle assembly and speed were normal in dyf13 mutant flagella, unlike in other IFT complex B mutants. Proteomic and biochemical analyses indicated a particular set of proteins involved in motility was specifically depleted in the dyf13 mutant. These results support the concept that different IFT proteins are responsible for different cargo subsets, providing a possible explanation for the complexity of the IFT machinery. DOI: http://dx.doi.org/10.7554/eLife.01566.001 PMID:24596149

  19. A Novel High Content Imaging-Based Screen Identifies the Anti-Helminthic Niclosamide as an Inhibitor of Lysosome Anterograde Trafficking and Prostate Cancer Cell Invasion.

    Directory of Open Access Journals (Sweden)

    Magdalena L Circu

    Full Text Available Lysosome trafficking plays a significant role in tumor invasion, a key event for the development of metastasis. Previous studies from our laboratory have demonstrated that the anterograde (outward movement of lysosomes to the cell surface in response to certain tumor microenvironment stimulus, such as hepatocyte growth factor (HGF or acidic extracellular pH (pHe, increases cathepsin B secretion and tumor cell invasion. Anterograde lysosome trafficking depends on sodium-proton exchanger activity and can be reversed by blocking these ion pumps with Troglitazone or EIPA. Since these drugs cannot be advanced into the clinic due to toxicity, we have designed a high-content assay to discover drugs that block peripheral lysosome trafficking with the goal of identifying novel drugs that inhibit tumor cell invasion. An automated high-content imaging system (Cellomics was used to measure the position of lysosomes relative to the nucleus. Among a total of 2210 repurposed and natural product drugs screened, 18 "hits" were identified. One of the compounds identified as an anterograde lysosome trafficking inhibitor was niclosamide, a marketed human anti-helminthic drug. Further studies revealed that niclosamide blocked acidic pHe, HGF, and epidermal growth factor (EGF-induced anterograde lysosome redistribution, protease secretion, motility, and invasion of DU145 castrate resistant prostate cancer cells at clinically relevant concentrations. In an effort to identify the mechanism by which niclosamide prevented anterograde lysosome movement, we found that this drug exhibited no significant effect on the level of ATP, microtubules or actin filaments, and had minimal effect on the PI3K and MAPK pathways. Niclosamide collapsed intralysosomal pH without disruption of the lysosome membrane, while bafilomycin, an agent that impairs lysosome acidification, was also found to induce JLA in our model. Taken together, these data suggest that niclosamide promotes

  20. CFTR is required for maximal transepithelial liquid transport in pig alveolar epithelia

    OpenAIRE

    Li, Xiaopeng; Comellas, Alejandro P.; Karp, Philip H.; Ernst, Sarah E.; Moninger, Thomas O.; Gansemer, Nicholas D.; Taft, Peter J.; Pezzulo, Alejandro A; Michael V Rector; Rossen, Nathan; Stoltz, David A.; McCray, Paul B.; Welsh, Michael J.; Zabner, Joseph

    2012-01-01

    A balance between alveolar liquid absorption and secretion is critical for maintaining optimal alveolar subphase liquid height and facilitating gas exchange in the alveolar space. However, the role of cystic fibrosis transmembrane regulator protein (CFTR) in this homeostatic process has remained elusive. Using a newly developed porcine model of cystic fibrosis, in which CFTR is absent, we investigated ion transport properties and alveolar liquid transport in isolated type II alveolar epitheli...

  1. Astrocyte-neuron lactate transport is required for long-term memory formation

    OpenAIRE

    Suzuki, Akinobu; Stern, Sarah A.; Bozdagi, Ozlem; Huntley, George W.; Walker, Ruth H.; Magistretti, Pierre J.; Alberini, Cristina M

    2011-01-01

    We report that in the rat hippocampus learning leads to a significant increase in extracellular lactate levels, which derive from glycogen, an energy reserve selectively localized in astrocytes. Astrocytic glycogen breakdown and lactate release are essential for long-term but not short-term memory formation, and for the maintenance of long-term potentiation (LTP) of synaptic strength elicited in-vivo. Disrupting the expression of the astrocytic lactate transporters monocarboxylate transporter...

  2. General requirement for harvesting antennae at Ca2+ and H+ channels and transporters

    Directory of Open Access Journals (Sweden)

    Cristián Martínez

    2010-09-01

    Full Text Available The production and dissipation of energy in cells is intimately linked to the movement of small molecules in and out of enzymes, channels and transporters. An analytical model of diffusion was described previously, which was used to estimate local effects of these proteins acting as molecular sources. The present article describes a simple but more general model, which can be used to estimate the local impact of proteins acting as molecular sinks. The results show that the enzymes, transporters and channels, whose substrates are present at relatively high concentrations like ATP, Na+, glucose, lactate and pyruvate, do not operate fast enough to deplete their vicinity to a meaningful extent, supporting the notion that for these molecules the cytosol is a well-mixed compartment. One specific consequence of this analysis is that the well-documented cross-talk existing between the Na+/K+ ATPase and the glycolytic machinery should not be explained by putative changes in local ATP concentration. In contrast, Ca2+ and H+ transporters like the Na+/Ca2+ exchanger NCX and the Na+/H+ exchanger NHE, show experimental rates of transport that are two to three orders of magnitude faster than the rates at which the aqueous phase may possibly feed their binding sites. This paradoxical result implies that Ca2+ and H+ transporters do not extract their substrates directly from the bulk cytosol, but from an intermediate “harvesting” compartment located between the aqueous phase and the transport site.

  3. Transportation of radioactive materials: a summary of state and local legislative requirements for the period ending December 31, 1985

    International Nuclear Information System (INIS)

    This report lists 670 adopted US state and local laws that impact the transportation of radioactive materials. The report was generated from information contained in the Legislative Database (LDB), a comprehensive, interactive database developed at the Oak Ridge National Laboratory for the US Department of Energy and the Joint Integration Office. Laws are sorted alphabetically by state, with state and local bills listed separately and sorted by date of adoption. Each citation contains the following information: locale (geographical areas and political jurisdictions affected by the action), bill number, bill title, bill sponsor, history of bill status, comments, and abstract. Seven indexes are provided to assist the reader in locating legislation of interest: locale, bill number, title word (permuted), sponsor, transport restriction (type of transportation restriction specified, e.g., escort, notify, permit, ban), transport mode (mode of transportation specified, e.g., truck, rail, barge), and keyword. This report adds new legislation to the information contained in last year's report, ''Transportation of Radioactive and Hazardous Materials: A Summary of State and Local Legislative Requirements for the Period Ending December 31, 1981,'' ORNL/TM-9563, published in September 1985

  4. 军用运输机集装运输托盘需求分析%Analysis of General Requirement of Military Transport Airplane Container Transport Pallets

    Institute of Scientific and Technical Information of China (English)

    张家应; 刘子明

    2015-01-01

    对军用运输机集装运输托盘研制的业务功能、保障环境、适用机型和技术规范标准等方面的需求进行初步分析,提出了军用运输机集装运输托盘研制需求的详细内容。随着托盘设计研究的不断深入,有关需求的内容与标准要求还应随之不断补充调整。%In order to provide some theoretical references for the development of military transport airplane container transport pallet, the requirements on every aspect of the development military transport airplane container transport pallet, including its operational functions,support environment,suitable model,tactical and technical capabilities performance indexes, specifications,were analyzed and discussed in this paper.

  5. Cuban experience in verification of the execution of the safety requirements during the transport of radioactive materials

    International Nuclear Information System (INIS)

    The Cuban Regulatory Authority has paid special attention to the verification of the execution of the safety requirements during the transport of radioactive material in the country. With this purpose, the Authority has followed a consequent policy based on supplementary demands to those collections in the juridical mark settled down in 1987 in the sphere of transport of radioactive substances. In the work the technical approaches are exposed kept in mind when establishing the one referred politics, the current situation is characterized, the results are evaluated obtained in correspondence with the pursued objectives and the essential aspects are exposed to keep in mind for the adopted politics ulterior development. (author)

  6. Classification of the railway accident in accordance with the requirement of the safety analysis of transporting spent fuel

    International Nuclear Information System (INIS)

    Based on the analysis of the difference between the accident severity categorization used in the Ministry of Railway and that used in the safety analysis of the transporting spent fuel, a method used for the classification of the railway accident in accordance with the requirement of the safety analysis of transporting spent fuel is suggested. The method classifies the railway accidents into 10 scenarios and make it possible to scale the accident through directly using the data documented by the Ministry of Railway without any additional effort

  7. Different types of spinal afferent nerve endings in stomach and esophagus identified by anterograde tracing from dorsal root ganglia.

    Science.gov (United States)

    Spencer, Nick J; Kyloh, Melinda; Beckett, Elizabeth A; Brookes, Simon; Hibberd, Tim

    2016-10-15

    In visceral organs of mammals, most noxious (painful) stimuli as well as innocuous stimuli are detected by spinal afferent neurons, whose cell bodies lie in dorsal root ganglia (DRGs). One of the major unresolved questions is the location, morphology, and neurochemistry of the nerve endings of spinal afferents that actually detect these stimuli in the viscera. In the upper gastrointestinal (GI) tract, there have been many anterograde tracing studies of vagal afferent endings, but none on spinal afferent endings. Recently, we developed a technique that now provides selective labeling of only spinal afferents. We used this approach to identify spinal afferent nerve endings in the upper GI tract of mice. Animals were anesthetized, and injections of dextran-amine were made into thoracic DRGs (T8-T12). Seven days post surgery, mice were euthanized, and the stomach and esophagus were removed, fixed, and stained for calcitonin gene-related peptide (CGRP). Spinal afferent axons were identified that ramified extensively through many rows of myenteric ganglia and formed nerve endings in discrete anatomical layers. Most commonly, intraganglionic varicose endings (IGVEs) were identified in myenteric ganglia of the stomach and varicose simple-type endings in the circular muscle and mucosa. Less commonly, nerve endings were identified in internodal strands, blood vessels, submucosal ganglia, and longitudinal muscle. In the esophagus, only IGVEs were identified in myenteric ganglia. No intraganglionic lamellar endings (IGLEs) were identified in the stomach or esophagus. We present the first identification of spinal afferent endings in the upper GI tract. Eight distinct types of spinal afferent endings were identified in the stomach, and most of them were CGRP immunoreactive. J. Comp. Neurol. 524:3064-3083, 2016. © 2016 Wiley Periodicals, Inc. PMID:27019197

  8. Brainstem projections of neurons located in various subdivisions of the dorsolateral hypothalamic area – an anterograde tract-tracing study

    Directory of Open Access Journals (Sweden)

    Rege Sugárka Papp

    2014-05-01

    Full Text Available The projections from the dorsolateral hypothalamic area (DLH to the lower brainstem have been investigated by using biotinylated dextran amine (BDA, an anterograde tracer in rats. The DLH can be divided into 3 areas (dorsomedial hypothalamus, perifornical area, lateral hypothalamic area, and further subdivided into 8 subdivisions. After unilateral stereotaxic injections of BDA into individual DLH subdivisions, the correct sites of injections were controlled histologically, and the distribution patterns of BDA-positive fibers were mapped on serial sections between the hypothalamus and spinal cord in 22 rats. BDA-labeled fibers were observable over 100 different brainstem areas, nuclei or subdivisions. Injections into the 8 DLH subdivisions established distinct topographical patterns. In general, the density of labeled fibers was low in the lower brainstem. High density of fibers was seen only 4 of the 116 areas: in the lateral and ventrolateral parts of the periaqueductal gray, the Barrington’s and the pedunculopontine tegmental nuclei. All of the biogenic amine cell groups in the lower brainstem (9 noradrenaline, 3 adrenaline and 9 serotonin cell groups received labeled fibers, some of them from all, or at least 7 DLH subdivisions, mainly from perifornical and ventral lateral hypothalamic neurons. Some of the tegmental nuclei and nuclei of the reticular formation were widely innervated, although the density of the BDA-labeled fibers was generally low. No definitive descending BDA-positive pathway, but long-run solitaire BDA-labeled fibers were seen in the lower brainstem. These descending fibers joined some of the large tracts or fasciculi in the brainstem. The distribution pattern of BDA-positive fibers of DLH origin throughout the lower brainstem was comparable to patterns of previously published orexin- or melanin-concentrating hormone-immunoreactive fibers with somewhat differences.

  9. Molecular and immunological analysis of an ABC transporter complex required for cytochrome c biogenesis.

    Science.gov (United States)

    Goldman, B S; Beckman, D L; Bali, A; Monika, E M; Gabbert, K K; Kranz, R G

    1997-05-16

    The helABC genes are predicted to encode an ATP-binding cassette (ABC) transporter necessary for heme export for ligation in bacterial cytochrome c biogenesis. The recent discoveries of homologs of the helB and helC genes in plant mitochondrial genomes suggest this is a highly conserved transporter in prokaryotes and some eukaryotes with the HelB and HelC proteins comprising the transmembrane components. Molecular genetic analysis in the Gram-negative bacterium Rhodobacter capsulatus was used to show that the helABC and helDX genes are part of an operon linked to the secDF genes. To facilitate analysis of this transporter, strains with non-polar deletions in each gene, epitope and reporter-tagged HelABCD proteins, and antisera specific to the HelA and HelX proteins were generated. We directly demonstrate that this transporter is present in the cytoplasmic membrane as an HelABCD complex. The HelB and HelC but not HelD proteins are necessary for the binding and stability of the HelA protein, the cytoplasmic subunit containing the ATP-binding region. In addition we show that the HelA protein co-immunoprecipitates with either the HelC or HelD proteins. Thus, the HelABCD heme export complex is distinguished by the presence of four membrane-associated subunits and represents a unique subfamily of ABC transporters. PMID:9175857

  10. Glibenclamide dose response in patients with septic shock: effects on norepinephrine requirements, cardiopulmonary performance, and global oxygen transport.

    Science.gov (United States)

    Morelli, Andrea; Lange, Matthias; Ertmer, Christian; Broeking, Katrin; Van Aken, Hugo; Orecchioni, Alessandra; Rocco, Monica; Bachetoni, Alessandra; Traber, Daniel L; Landoni, Giovanni; Pietropaoli, Paolo; Westphal, Martin

    2007-11-01

    Adenosine triphosphate-sensitive potassium channels are important regulators of arterial vascular smooth muscle tone and are implicated in the pathophysiology of catecholamine tachyphylaxis in septic shock. The present study was designed as a prospective, randomized, double-blinded, clinical pilot study to determine whether different doses of glibenclamide have any effects on norepinephrine requirements, cardiopulmonary hemodynamics, and global oxygen transport in patients with septic shock. We enrolled 30 patients with septic shock requiring invasive hemodynamic monitoring and norepinephrine infusion of 0.5 microg.kg-1.min-1 or greater to maintain MAP between 65 and 75 mmHg. In addition to standard therapy, patients were randomized to receive either 10, 20, or 30 mg of enteral glibenclamide. Systemic hemodynamics, global oxygen transport including arterial lactate concentrations, gas exchange, plasma glucose concentrations, and electrolytes were determined at baseline and after 3, 6, and 12 h after administration of the study drug. Glibenclamide decreased plasma glucose concentrations in a dose-dependent manner but failed to reduce norepinephrine requirements. None of the doses had any effects on cardiopulmonary hemodynamics, global oxygen transport, gas exchange, or electrolytes. These data suggest that oral glibenclamide in doses from 10 to 30 mg fails to counteract arterial hypotension and thus to reduce norepinephrine requirements in catecholamine-dependent human septic shock.

  11. The N terminus of monoamine transporters is a lever required for the action of amphetamines

    DEFF Research Database (Denmark)

    Sucic, Sonja; Dallinger, Stefan; Zdrazil, Barbara;

    2010-01-01

    The serotonin transporter (SERT) terminates neurotransmission by removing serotonin from the synaptic cleft. In addition, it is the site of action of antidepressants (which block the transporter) and of amphetamines (which induce substrate efflux). We explored the functional importance of the N...... terminus in mediating the action of amphetamines by focusing initially on the highly conserved threonine residue at position 81, a candidate site for phosphorylation by protein kinase C. Molecular dynamics simulations of the wild type SERT, compared with its mutations SERT(T81A) and SERT(T81D), suggested......, SERT(T81A) (and the homologous mutations in noradrenaline and dopamine) failed to support amphetamine-induced efflux, and this was not remedied by aspartate at this position. Amphetamine-induced currents through SERT(T81A) were comparable with those through the wild type transporter. Both abundant Na...

  12. Ecological hydraulic radius model to calculate instream flow requirements for transporting sediment in the western water transfer region

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    Transporting sediment is a natural function of the river. To maintain the normal evolution of the river certain amount of water flow is required, which is called the instream flow requirements for transporting sediment (IFRTS). We defined the permitting flow velocity by the conception of IFRTS, and also put forward the ecological hydraulic radius model (EHRM) to estimate IFRTS. The calculating process of EHRM is explained by the example of Daofu Hydrological Station on Xianshui branch of Yalong River in the west line first-stage construction of South-North Water Transfer Project. The result shows that the IFRTS occupied 29.7%―59.5% of annual mean discharge in flood season, the average of IFRTS was about 100.2 m3/s during 1966―1987, it is close to the IFRTS 90 m3/s calculated by IFRTS conception. Hence, it is feasible to use EHRM to calculate IFRTS.

  13. Spent fuel storage and transport cask decontamination and modification. An overview of management requirements and applications based on practical experience

    International Nuclear Information System (INIS)

    A large increase in the number of casks required for transport and/or storage of spent fuel is forecast into the next century. The principal requirement will be for increased number of storage and dual purpose (transport/storage) casks for interim storage of spent fuel prior to reprocessing or permanent disposal in both on-site and off-site storage facilities. Through contact with radioactive materials spent fuel casks will be contaminated on both internal and external surfaces. In broad terms, cask contamination management can be defined by three components: minimisation, prevention and decontamination. This publication is a compilation of international experience with cask contamination problems and decontamination practices. The objective is to present current knowledge and experience as well as developments, trends and potential for new applications in this field. Furthermore, the report may assist in new design or modification of existing casks, cask handling systems and decontamination equipment

  14. 77 FR 17394 - Hazardous Materials: Approval and Communication Requirements for the Safe Transportation of Air...

    Science.gov (United States)

    2012-03-26

    ... Department of Transportation (44 FR 11034). Executive Order 13563 is supplemental to and reaffirms the... tape or a waterproof soft plastic cap that must easily provide ventilation of the drum contents in the... has substantial direct effects on the States, the relationship between the national government and...

  15. Hxt1, a monosaccharide transporter and sensor required for virulence of the maize pathogen Ustilago maydis.

    Science.gov (United States)

    Schuler, David; Wahl, Ramon; Wippel, Kathrin; Vranes, Miroslav; Münsterkötter, Martin; Sauer, Norbert; Kämper, Jörg

    2015-05-01

    The smut Ustilago maydis, a ubiquitous pest of corn, is highly adapted to its host to parasitize on its organic carbon sources. We have identified a hexose transporter, Hxt1, as important for fungal development during both the saprophytic and the pathogenic stage of the fungus. Hxt1 was characterized as a high-affinity transporter for glucose, fructose, and mannose; ∆hxt1 strains show significantly reduced growth on these substrates, setting Hxt1 as the main hexose transporter during saprophytic growth. After plant infection, ∆hxt1 strains show decreased symptom development. However, expression of a Hxt1 protein with a mutation leading to constitutively active signaling in the yeast glucose sensors Snf3p and Rgt2p results in completely apathogenic strains. Fungal development is stalled immediately after plant penetration, implying a dual function of Hxt1 as transporter and sensor. As glucose sensors are only known for yeasts, 'transceptor' as Hxt1 may constitute a general mechanism for sensing of glucose in fungi. In U. maydis, Hxt1 links a nutrient-dependent environmental signal to the developmental program during pathogenic development.

  16. Establishing Sprinkling Requirements on Trailers Transporting Market Weight Pigs in Warm and Hot Weather

    Directory of Open Access Journals (Sweden)

    Rebecca Kephart

    2014-04-01

    Full Text Available This study was conducted July of 2012 in Iowa, in WARM (<26.7 °C and HOT (≥26.7 °C weather. Four sprinkling methods were compared, with one treatment being randomly assigned to each load: control- no sprinkling (not applied in HOT weather, pigs only, bedding only, or pigs and bedding. Experiment 1 used 51 loads in WARM- and 86 loads in HOT weather to determine sprinkling effects on pig measures (surface temperature, vocalizations, slips and falls, and stress signs. Experiment 2 used 82 loads in WARM- and 54 loads in HOT weather to determine the sprinkling effects on transport losses (non-ambulatory, dead, and total transport losses. Experiment 1 found that, in WARM weather, there were no differences between sprinkling treatments for surface temperature, vocalizations, or slips and falls (p ≥ 0.18. However, stress signs were 2% greater when sprinkling pigs- or bedding only- compared to control (p = 0.03. Experiment 2 found that, in WARM and HOT weather, sprinkling did not affect non-ambulatory, dead, or total transport losses (p ≥ 0.18. Although the current study did not find any observed sprinkling effects for pig measures or transport losses it is extremely important to note that the inference space of this study is relatively small, so further studies should be conducted to see if these results are applicable to other geographical regions and seasons.

  17. 75 FR 10973 - Hazardous Materials: Risk-Based Adjustment of Transportation Security Plan Requirements

    Science.gov (United States)

    2010-03-09

    ... to address the security of hazardous materials shipments.'' 68 FR 14511. PHMSA noted in the NPRM that... to evaluate the transportation security risks posed by all types of hazardous materials and the... disagree with some of the types and classes of materials that would be subject to security...

  18. Repellent Taxis in Response to Nickel Ion Requires neither Ni2+ Transport nor the Periplasmic NikA Binding Protein▿

    OpenAIRE

    Englert, Derek L.; Adase, Christopher A.; Jayaraman, Arul; Manson, Michael D.

    2010-01-01

    Ni2+ and Co2+ are sensed as repellents by the Escherichia coli Tar chemoreceptor. The periplasmic Ni2+ binding protein, NikA, has been suggested to sense Ni2+. We show here that neither NikA nor the membrane-bound NikB and NikC proteins of the Ni2+ transport system are required for repellent taxis in response to Ni2+.

  19. Review of data requirements for groundwater flow and solute transport modelling and the ability of site investigation methods to meet these requirements

    International Nuclear Information System (INIS)

    This report describes the data requirements for the codes that may be used in the modelling of groundwater flow and radionuclide transport during the assessment of a Nirex site for the deep disposal of low and intermediate level radioactive waste and also the site investigation methods that exist to supply the data for these codes. The data requirements for eight codes are reviewed, with most emphasis on three of the more significant codes, VANDAL, NAMMU and CHEMTARD. The largest part of the report describes and discusses the site investigation techniques and each technique is considered in terms of its ability to provide the data necessary to characterise the geological and hydrogeological environment around a potential repository. (author)

  20. Adult active transport in the Netherlands: an analysis of its contribution to physical activity requirements.

    Directory of Open Access Journals (Sweden)

    Elliot Fishman

    Full Text Available Modern, urban lifestyles have engineered physical activity out of everyday life and this presents a major threat to human health. The Netherlands is a world leader in active travel, particularly cycling, but little research has sought to quantify the cumulative amount of physical activity through everyday walking and cycling.Using data collected as part of the Dutch National Travel Survey (2010 - 2012, this paper determines the degree to which Dutch walking and cycling contributes to meeting minimum level of physical activity of 150 minutes of moderate intensity aerobic activity throughout the week. The sample includes 74,465 individuals who recorded at least some travel on the day surveyed. As physical activity benefits are cumulative, all walking and cycling trips are analysed, including those to and from public transport. These trips are then converted into an established measure of physical activity intensity, known as metabolic equivalents of tasks. Multivariate Tobit regression models were performed on a range of socio-demographic, transport resources, urban form and meteorological characteristics.The results reveal that Dutch men and women participate in 24 and 28 minutes of daily physical activity through walking and cycling, which is 41% and 55% more than the minimum recommended level. It should be noted however that some 57% of the entire sample failed to record any walking or cycling, and an investigation of this particular group serves as an important topic of future research. Active transport was positively related with age, income, bicycle ownership, urban density and air temperature. Car ownership had a strong negative relationship with physically active travel.The results of this analysis demonstrate the significance of active transport to counter the emerging issue of sedentary lifestyle disease. The Dutch experience provides other countries with a highly relevant case study in the creation of environments and cultures that

  1. Review and assessment of package requirements (yellowcake) and emergency response to transportation accidents

    International Nuclear Information System (INIS)

    As a consequence of an accident involving a truck shipment of yellowcake, a joint NRC--DOT study was undertaken to review and assess the regulations and practices related to package integrity and to emergency response to transportation accidents involving low specific activity radioactive materials. Recommendations are made regarding the responsibilities of state and local agencies, carriers, and shippers, and the DOT and NRC regulations

  2. Assessment of Quality Assurance Measures for Radioactive Material Transport Packages not Requiring Competent Authority Design Approval - 13282

    Energy Technology Data Exchange (ETDEWEB)

    Komann, Steffen; Groeke, Carsten; Droste, Bernhard [BAM Federal Institute for Materials Research and Testing, Unter den Eichen 44-46, 12203 Berlin (Germany)

    2013-07-01

    The majority of transports of radioactive materials are carried out in packages which don't need a package design approval by a competent authority. Low-active radioactive materials are transported in such packages e.g. in the medical and pharmaceutical industry and in the nuclear industry as well. Decommissioning of NPP's leads to a strong demand for packages to transport low and middle active radioactive waste. According to IAEA regulations the 'non-competent authority approved package types' are the Excepted Packages and the Industrial Packages of Type IP-1, IP-2 and IP-3 and packages of Type A. For these types of packages an assessment by the competent authority is required for the quality assurance measures for the design, manufacture, testing, documentation, use, maintenance and inspection (IAEA SSR 6, Chap. 306). In general a compliance audit of the manufacturer of the packaging is required during this assessment procedure. Their regulatory level in the IAEA regulations is not comparable with the 'regulatory density' for packages requiring competent authority package design approval. Practices in different countries lead to different approaches within the assessment of the quality assurance measures in the management system as well as in the quality assurance program of a special package design. To use the package or packaging in a safe manner and in compliance with the regulations a management system for each phase of the life of the package or packaging is necessary. The relevant IAEA-SSR6 chap. 801 requires documentary verification by the consignor concerning package compliance with the requirements. (authors)

  3. Assessment of Quality Assurance Measures for Radioactive Material Transport Packages not Requiring Competent Authority Design Approval - 13282

    International Nuclear Information System (INIS)

    The majority of transports of radioactive materials are carried out in packages which don't need a package design approval by a competent authority. Low-active radioactive materials are transported in such packages e.g. in the medical and pharmaceutical industry and in the nuclear industry as well. Decommissioning of NPP's leads to a strong demand for packages to transport low and middle active radioactive waste. According to IAEA regulations the 'non-competent authority approved package types' are the Excepted Packages and the Industrial Packages of Type IP-1, IP-2 and IP-3 and packages of Type A. For these types of packages an assessment by the competent authority is required for the quality assurance measures for the design, manufacture, testing, documentation, use, maintenance and inspection (IAEA SSR 6, Chap. 306). In general a compliance audit of the manufacturer of the packaging is required during this assessment procedure. Their regulatory level in the IAEA regulations is not comparable with the 'regulatory density' for packages requiring competent authority package design approval. Practices in different countries lead to different approaches within the assessment of the quality assurance measures in the management system as well as in the quality assurance program of a special package design. To use the package or packaging in a safe manner and in compliance with the regulations a management system for each phase of the life of the package or packaging is necessary. The relevant IAEA-SSR6 chap. 801 requires documentary verification by the consignor concerning package compliance with the requirements. (authors)

  4. A monocarboxylate transporter required for hepatocyte secretion of ketone bodies during fasting.

    Science.gov (United States)

    Hugo, Sarah E; Cruz-Garcia, Lourdes; Karanth, Santhosh; Anderson, Ryan M; Stainier, Didier Y R; Schlegel, Amnon

    2012-02-01

    To find new genes that influence liver lipid mass, we performed a genetic screen for zebrafish mutants with hepatic steatosis, a pathological accumulation of fat. The red moon (rmn) mutant develops hepatic steatosis as maternally deposited yolk is depleted. Conversely, hepatic steatosis is suppressed in rmn mutants by adequate nutrition. Adult rmn mutants show increased liver neutral lipids and induction of hepatic lipid biosynthetic genes when fasted. Positional cloning of the rmn locus reveals a loss-of-function mutation in slc16a6a (solute carrier family 16a, member 6a), a gene that we show encodes a transporter of the major ketone body β-hydroxybutyrate. Restoring wild-type zebrafish slc16a6a expression or introducing human SLC16A6 in rmn mutant livers rescues the mutant phenotype. Radiotracer analysis confirms that loss of Slc16a6a function causes diversion of liver-trapped ketogenic precursors into triacylglycerol. Underscoring the importance of Slc16a6a to normal fasting physiology, previously fed rmn mutants are more sensitive to death by starvation than are wild-type larvae. Our unbiased, forward genetic approach has found a heretofore unrecognized critical step in fasting energy metabolism: hepatic ketone body transport. Since β-hydroxybutyrate is both a major fuel and a signaling molecule in fasting, the discovery of this transporter provides a new direction for modulating circulating levels of ketone bodies in metabolic diseases.

  5. Transport of the outer dynein arm complex to cilia requires a cytoplasmic protein Lrrc6.

    Science.gov (United States)

    Inaba, Yasuko; Shinohara, Kyosuke; Botilde, Yanick; Nabeshima, Ryo; Takaoka, Katsuyoshi; Ajima, Rieko; Lamri, Lynda; Takeda, Hiroyuki; Saga, Yumiko; Nakamura, Tetsuya; Hamada, Hiroshi

    2016-07-01

    Lrrc6 encodes a cytoplasmic protein that is expressed specifically in cells with motile cilia including the node, trachea and testes of the mice. A mutation of Lrrc6 has been identified in human patients with primary ciliary dyskinesia (PCD). Mutant mice lacking Lrrc6 show typical PCD defects such as hydrocephalus and laterality defects. We found that in the absence of Lrrc6, the morphology of motile cilia remained normal, but their motility was completely lost. The 9 + 2 arrangement of microtubules remained normal in Lrrc6(-/-) mice, but the outer dynein arms (ODAs), the structures essential for the ciliary beating, were absent from the cilia. In the absence of Lrrc6, ODA proteins such as DNAH5, DNAH9 and IC2, which are assembled in the cytoplasm and transported to the ciliary axoneme, remained in the cytoplasm and were not transported to the ciliary axoneme. The IC2-IC1 interaction, which is the first step of ODA assembly, was normal in Lrrc6(-/-) mice testes. Our results suggest that ODA proteins may be transported from the cytoplasm to the cilia by an Lrrc6-dependent mechanism.

  6. Regional, national and international security requirements for the transport of nuclear cargo by sea

    International Nuclear Information System (INIS)

    Since the beginning of the nuclear age in the 1940's, the world has focused on the immense possibilities of nuclear power with both its destructive and productive capabilities. The civil nuclear industry in the UK, as in most nuclear weapons states, grew from the military facilities built in the post war years under the political climate of the Cold War. In the early years of the industry, civil and defence nuclear facilities were inextricably linked both in public perceptions and the regulatory infrastructure under which they operated. The nuclear arms race and the spread of communism overshadowed people's perceptions of there being two separate uses of nuclear material. This was a double edged sword which initially allowed the industry to develop largely unhindered by public concerns but latterly meant the industry could not break away from its roots and to many is still perceived as a dangerous and destructive force. Regulatory frameworks governing all aspects of the industry have developed both nationally and internationally driven by valid public concerns, political agendas and an international consensus that the unregulated use of nuclear material has catastrophic possibilities on an international scale. With the internationalisation of the civil nuclear industry and the costs associated with developing facilities to fully support each stage of the fuel cycle, from enrichment, fuel manufacturing, reprocessing and waste remediation, it became inevitable that a transport infrastructure would develop to make best use of the facilities. Regulations, both national and international are implicit in ensuring the security of nuclear material in transit. Due to the physical size of many of the irradiated fuel packages and implications of the changes to transport safety regulations, international transports of nuclear material, other than within mainland Europe, is predominantly carried out by sea

  7. Regional, national and international security requirements for the transport of nuclear cargo by sea

    Energy Technology Data Exchange (ETDEWEB)

    Booker, P.A.; Barnwell, I. [Marine Operations, BNFL International Transport and British Nuclear Group Security (United Kingdom)

    2004-07-01

    Since the beginning of the nuclear age in the 1940's, the world has focused on the immense possibilities of nuclear power with both its destructive and productive capabilities. The civil nuclear industry in the UK, as in most nuclear weapons states, grew from the military facilities built in the post war years under the political climate of the Cold War. In the early years of the industry, civil and defence nuclear facilities were inextricably linked both in public perceptions and the regulatory infrastructure under which they operated. The nuclear arms race and the spread of communism overshadowed people's perceptions of there being two separate uses of nuclear material. This was a double edged sword which initially allowed the industry to develop largely unhindered by public concerns but latterly meant the industry could not break away from its roots and to many is still perceived as a dangerous and destructive force. Regulatory frameworks governing all aspects of the industry have developed both nationally and internationally driven by valid public concerns, political agendas and an international consensus that the unregulated use of nuclear material has catastrophic possibilities on an international scale. With the internationalisation of the civil nuclear industry and the costs associated with developing facilities to fully support each stage of the fuel cycle, from enrichment, fuel manufacturing, reprocessing and waste remediation, it became inevitable that a transport infrastructure would develop to make best use of the facilities. Regulations, both national and international are implicit in ensuring the security of nuclear material in transit. Due to the physical size of many of the irradiated fuel packages and implications of the changes to transport safety regulations, international transports of nuclear material, other than within mainland Europe, is predominantly carried out by sea.

  8. Siting of an MRS facility: identification of a geographic region that reduces transportation requirements

    Energy Technology Data Exchange (ETDEWEB)

    Holter, G.M.; Braitman, J.L.

    1985-04-01

    The study reported here was undertaken as part of the site screening and evaluation activities for the Monitored Retrievable Storage (MRS) Program of the Office of Civilian Radioactive Waste Management (OCRWM), Department of Energy (DOE). Its primary purpose was to determine: the location and shape of a preferred geographic region within which locating an MRS facility would minimize total shipment miles for spent fuel transported through the MRS facility to a repository, and the sensitivity of the location and shape of this region and the reduction in total shipment miles to possible variations in waste management system logistics. As a result of this analysis, a geographic region has been identified which is preferred for siting an MRS facility. This region will be referred to as the preferred region in this study. Siting an MRS facility in the preferred region will limit total shipment miles (i.e., the total miles traveled for all shipments of spent fuel) to and from the MRS facility to within 20% of the lowest achievable. The region is preferred for a mixed truck/rail system of transport from reactors to the MRS facility. It is assumed that rail will be used to ship spent fuel from the MRS facility to a geologic repository for disposal. Siting an MRS facility in the preferred region will reduce total shipment miles for all currently considered system logistics options which include an MRS facility in the system. These options include: any first repository location, the possible range of spent fuel consolidation at the MRS, use of multi-cask or single-cask train shipments, use of current or future spent fuel transport casks, servicing only the first or both the first and second repositories, and shipment of fuel from western reactors either through the MRS facility or to a western facility (a second, smaller MRS facility or the first repository).

  9. SGLT1 sugar transporter/sensor is required for post-oral glucose appetition.

    Science.gov (United States)

    Sclafani, Anthony; Koepsell, Hermann; Ackroff, Karen

    2016-04-01

    Recent findings suggest that the intestinal sodium-glucose transporter 1 (SGLT1) glucose transporter and sensor mediates, in part, the appetite-stimulation actions of intragastric (IG) glucose and nonmetabolizable α-methyl-d-glucopyranoside (MDG) infusions in mice. Here, we investigated the role of SGLT1 in sugar conditioning using SGLT1 knockout (KO) and C57BL/6J wild-type (WT) mice. An initial experiment revealed that both KO and WT mice maintained on a very low-carbohydrate diet display normal preferences for saccharin, which was used in the flavored conditioned stimulus (CS) solutions. In experiment 2, mice were trained to drink one flavored solution (CS+) paired with an IG MDG infusion and a different flavored solution (CS-) paired with IG water infusion. In contrast to WT mice, KO mice decreased rather than increased the intake of the CS+ during training and failed to prefer the CS+ over the CS- in a choice test. In experiment 3, the KO mice also decreased their intake of a CS+ paired with IG glucose and avoided the CS+ in a choice test, unlike WT mice, which preferred the CS+ to CS-. In experiment 4, KO mice, like WT mice preferred a glucose + saccharin solution to a saccharin solution. These findings support the involvement of SGLT1 in post-oral glucose and MDG conditioning. The results also indicate that sugar malabsorption in KO mice has inhibitory effects on sugar intake but does not block their natural preference for sweet taste. PMID:26791832

  10. 32 CFR 861.4 - DOD air transportation quality and safety requirements.

    Science.gov (United States)

    2010-07-01

    ..., or 135 (14 CFR 121, 125, 127, or 135) air carrier (or foreign CAA equivalent if appropriate) on a...) Aircrew training. Training, including recurrent training, which develops and refines skills designed to... ensure that all maintenance tasks, including required inspections and airworthiness directives,...

  11. 76 FR 58227 - Passenger Vessel Operator Financial Responsibility Requirements for Nonperformance of Transportation

    Science.gov (United States)

    2011-09-20

    ... Regarding Passenger Vessel Responsibility, 74 FR 65125 (December 9, 2009). \\3\\ Federal Maritime Commission, Notice of Public Hearing, Passenger Vessel Financial Responsibility, 75 FR 7599 (February 22, 2010). A... Docket No. 90-01, Security for the Protection of the Public, Maximum Required Performance Amount, 55...

  12. Utilization of respiratory energy in higher plants. Requirements for 'maintenance' and transport processes.

    NARCIS (Netherlands)

    Bouma, T.J.

    1995-01-01

    Quantitative knowledge of both photosynthesis and respiration is required to understand plant growth and resulting crop yield. However, especially the nature of the energy demanding processes that are dependent on dark respiration in full-grown tissues is largely unknown. The main objective of the p

  13. 75 FR 43906 - Hazardous Materials: Requirements for the Storage of Explosives During Transportation

    Science.gov (United States)

    2010-07-27

    ... appropriate safety measures, because it assesses the unique characteristics of a particular site. DDESB... Fire Protection Association (NFPA) standard 498 as the Federally approved standard for the construction... rulemaking under Docket HM-232A (67 FR 46622) entitled ``Security Requirements for Motor...

  14. A Salmonella protein that is required for resistance to antimicrobial peptides and transport of potassium.

    OpenAIRE

    Parra-Lopez, C; Lin, R; Aspedon, A; Groisman, E A

    1994-01-01

    The ability of invading pathogens to proliferate within host tissues requires the capacity to resist the killing effects of a wide variety of host defense molecules. sap mutants of the facultative intracellular parasite Salmonella typhimurium exhibit hypersensitivity to antimicrobial peptides, cannot survive within macrophages in vitro and are attenuated for mouse virulence in vivo. We conducted a molecular genetic analysis of the sapG locus and showed that it encodes a product that is 99% id...

  15. A sodium requirement for growth, solute transport, and pH homeostasis in Bacillus firmus RAB.

    Science.gov (United States)

    Krulwich, T A; Guffanti, A A; Bornstein, R F; Hoffstein, J

    1982-02-25

    Activity of a Na+/H+ antiporter has been suggested to be critically involved in pH homeostasis in obligately alkalophilic bacteria (Krulwich, K. A., Mandel, K. G., Bornstein, R. F., and Guffanti, A. A. (1979) Biochem. Biophys. Res. Commun. 91, 58-62) and in Escherichia coli (Zilberstein, D., Padan, E., and Schuldiner, S. (1980) FEBS Lett. 116, 177-180). A concern with respect to these proposals has been the failure of either Bacillus alcalophilus or E. coli to exhibit a requirement for added Na+ for growth. Thus, it became of interest to examine Na+-coupled porter functions in obligately alkalophilic Bacillus firmus RAB, a species that exhibits an absolute requirement for added Na+ for growth at pH 10.5. In a comparative study using membrane vesicles from B. alcalophilus and B. firmus RAB it was found that both the Na+/H+ antiporter and the Na+/alpha-aminoisobutyric acid symporter from the "Na+-requiring" species had much lower apparent affinities for Na+ than corresponding porters from B. alcalophilus. At high concentrations of Na+, the porters from the two species were functionally similar. These findings support the argument that the absence of a growth requirement for added Na+ may reflect an ability of at least some bacteria to effectively utilize and recycle the available levels of Na+ that contaminate all media, rather than reflect true Na+ independence. Studies with a nonalkalophilic derivative of B. firmus RAB confirmed earlier findings with B. alcalophilus of a pleiotropic loss of Na+ coupling to porters in nonalkalophilic mutants. PMID:7056750

  16. A Feasibility Study for Gamification in Transport Maintenance : Requirements to implement gamification in heterogeneous organizations

    OpenAIRE

    Shreenath, Vinutha Magal; Kornevs, Maksims; Raghothama, Jayanth; Meijer, Sebastiaan

    2015-01-01

    Gamification has been successfully applied in many domains, but mostly for simple, isolated and operational tasks. The hope for gamification as a method to radically change and improve behavior, to provide incentives for sustained engagement has proven to be more difficult to get right. Applying gamification in large networked organizations with heterogeneous tasks remains a challenge. Applying gamification in such enterprise environments posits different requirements, and a match between the...

  17. Requirements for Forming Efficient 3-D Charge Transport Pathway in Diketopyrrolopyrrole-Based Copolymers: Film Morphology vs Molecular Packing.

    Science.gov (United States)

    Lee, Gang-Young; Han, A-Reum; Kim, Taewan; Lee, Hae Rang; Oh, Joon Hak; Park, Taiho

    2016-05-18

    To achieve extremely high planarity and processability simultaneously, we have newly designed and synthesized copolymers composed of donor units of 2,2'-(2,5-dialkoxy-1,4-phenylene)dithieno[3,2-b]thiophene (TT-P-TT) and acceptor units of diketopyrrolopyrrole (DPP). These copolymers consist of a highly planar backbone due to intramolecular interactions. We have systematically investigated the effects of intermolecular interactions by controlling the side chain bulkiness on the polymer thin-film morphologies, packing structures, and charge transport. The thin-film microstructures of the copolymers are found to be critically dependent upon subtle changes in the intermolecular interactions, and charge transport dynamics of the copolymer based field-effect transistors (FETs) has been investigated by in-depth structure-property relationship study. Although the size of the fibrillar structures increases as the bulkiness of the side chains in the copolymer increases, the copolymer with the smallest side chain shows remarkably high charge carrier mobility. Our findings reveal the requirement for forming efficient 3-D charge transport pathway and highlight the importance of the molecular packing and interdomain connectivity, rather than the crystalline domain size. The results obtained herein demonstrate the importance of tailoring the side chain bulkiness and provide new insights into the molecular design for high-performance polymer semiconductors. PMID:27117671

  18. Apical transport of influenza A virus ribonucleoprotein requires Rab11-positive recycling endosome.

    Directory of Open Access Journals (Sweden)

    Fumitaka Momose

    Full Text Available Influenza A virus RNA genome exists as eight-segmented ribonucleoprotein complexes containing viral RNA polymerase and nucleoprotein (vRNPs. Packaging of vRNPs and virus budding take place at the apical plasma membrane (APM. However, little is known about the molecular mechanisms of apical transport of newly synthesized vRNP. Transfection of fluorescent-labeled antibody and subsequent live cell imaging revealed that punctate vRNP signals moved along microtubules rapidly but intermittently in both directions, suggestive of vesicle trafficking. Using a series of Rab family protein, we demonstrated that progeny vRNP localized to recycling endosome (RE in an active/GTP-bound Rab11-dependent manner. The vRNP interacted with Rab11 through viral RNA polymerase. The localization of vRNP to RE and subsequent accumulation to the APM were impaired by overexpression of Rab binding domains (RBD of Rab11 family interacting proteins (Rab11-FIPs. Similarly, no APM accumulation was observed by overexpression of class II Rab11-FIP mutants lacking RBD. These results suggest that the progeny vRNP makes use of Rab11-dependent RE machinery for APM trafficking.

  19. myo-Inositol-1-phosphate synthase is required for polar auxin transport and organ development

    KAUST Repository

    Chen, Hao

    2010-06-01

    myo-Inositol-1-phosphate synthase is a conserved enzyme that catalyzes the first committed and rate-limiting step in inositol biosynthesis. Despite its wide occurrence in all eukaryotes, the role of myo-inositol-1-phosphate synthase and de novo inositol biosynthesis in cell signaling and organism development has been unclear. In this study, we isolated loss-of-function mutants in the Arabidopsis MIPS1 gene from different ecotypes. It was found that all mips1 mutants are defective in embryogenesis, cotyledon venation patterning, root growth, and root cap development. The mutant roots are also agravitropic and have reduced basipetal auxin transport. mips1 mutants have significantly reduced levels of major phosphatidylinositols and exhibit much slower rates of endocytosis. Treatment with brefeldin A induces slower PIN2 protein aggregation in mips1, indicating altered PIN2 trafficking. Our results demonstrate that MIPS1 is critical for maintaining phosphatidylinositol levels and affects pattern formation in plants likely through regulation of auxin distribution. © 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

  20. A Trypanosomatid Iron Transporter that Regulates Mitochondrial Function Is Required for Leishmania amazonensis Virulence.

    Directory of Open Access Journals (Sweden)

    Bidyottam Mittra

    2016-01-01

    Full Text Available Iron, an essential co-factor of respiratory chain proteins, is critical for mitochondrial function and maintenance of its redox balance. We previously reported a role for iron uptake in differentiation of Leishmania amazonensis into virulent amastigotes, by a mechanism that involves reactive oxygen species (ROS production and is independent of the classical pH and temperature cues. Iron import into mitochondria was proposed to be essential for this process, but evidence supporting this hypothesis was lacking because the Leishmania mitochondrial iron transporter was unknown. Here we describe MIT1, a homolog of the mitochondrial iron importer genes mrs3 (yeast and mitoferrin-1 (human that is highly conserved among trypanosomatids. MIT1 expression was essential for the survival of Trypanosoma brucei procyclic but not bloodstream forms, which lack functional respiratory complexes. L. amazonensis LMIT1 null mutants could not be generated, suggesting that this mitochondrial iron importer is essential for promastigote viability. Promastigotes lacking one LMIT1 allele (LMIT1/Δlmit1 showed growth defects and were more susceptible to ROS toxicity, consistent with the role of iron as the essential co-factor of trypanosomatid mitochondrial superoxide dismutases. LMIT1/Δlmit1 metacyclic promastigotes were unable to replicate as intracellular amastigotes after infecting macrophages or cause cutaneous lesions in mice. When induced to differentiate axenically into amastigotes, LMIT1/Δlmit1 showed strong defects in iron content and function of mitochondria, were unable to upregulate the ROS-regulatory enzyme FeSOD, and showed mitochondrial changes suggestive of redox imbalance. Our results demonstrate the importance of mitochondrial iron uptake in trypanosomatid parasites, and highlight the role of LMIT1 in the iron-regulated process that orchestrates differentiation of L. amazonensis into infective amastigotes.

  1. Model complexities and requirements for multimodal transport network design: Assessment of classical, state-of-the-practice, and state-of-the-research models

    NARCIS (Netherlands)

    Van Eck, G.; Brands, T.; Wismans, L.J.J.; Pel, A.J.; Van Nes, R.

    2014-01-01

    In the aim for a more sustainable transport system, governments try to stimulate multimodal trip making by facilitating smooth transfers between modes. The assessment of related multimodal policy measures requires transport models that are capable of handling the complex nature of multimodality. Thi

  2. A nucleotide sugar transporter involved in glycosylation of the Toxoplasma tissue cyst wall is required for efficient persistence of bradyzoites.

    Directory of Open Access Journals (Sweden)

    Carolina E Caffaro

    Full Text Available Toxoplasma gondii is an intracellular parasite that transitions from acute infection to a chronic infective state in its intermediate host via encystation, which enables the parasite to evade immune detection and clearance. It is widely accepted that the tissue cyst perimeter is highly and specifically decorated with glycan modifications; however, the role of these modifications in the establishment and persistence of chronic infection has not been investigated. Here we identify and biochemically and biologically characterize a Toxoplasma nucleotide-sugar transporter (TgNST1 that is required for cyst wall glycosylation. Toxoplasma strains deleted for the TgNST1 gene (Δnst1 form cyst-like structures in vitro but no longer interact with lectins, suggesting that Δnst1 strains are deficient in the transport and use of sugars for the biosynthesis of cyst-wall structures. In vivo infection experiments demonstrate that the lack of TgNST1 activity does not detectably impact the acute (tachyzoite stages of an infection or tropism of the parasite for the brain but that Δnst1 parasites are severely defective in persistence during the chronic stages of the infection. These results demonstrate for the first time the critical role of parasite glycoconjugates in the persistence of Toxoplasma tissue cysts.

  3. A component of the Sec61 ER protein transporting pore is required for plant susceptibility to powdery mildew

    DEFF Research Database (Denmark)

    Zhang, Wen-Jing; Hanisch, Susanne; Kwaaitaal, Mark Adrianus Cornelis J;

    2013-01-01

    and retrotranslocon pores, the latter being part of the ER-associated protein degradation machinery. We provide support for a model suggesting that the retrotranslocon function of HvSec61βa is required for successful powdery mildew fungal infection. HvSec61βa-GFP and a luminal ER marker were co-localized to the ER...... the EHM, the fungus obtains nutrients from and secretes effector proteins into the plant cell. In the plant cell these effectors interfere with cellular processes such as pathogen defense and membrane trafficking. However, the mechanisms behind effector delivery are largely unknown. This paper provides....... Effector transport across this EHM-ER interface may occur by a vesicle-mediated process, while the Sec61 retrotranslocon pore potentially provides an escape route for these proteins to reach the cytosol....

  4. Requirements for design of accelerator, beam transport, and target in a study of thermonuclear reaction cross section

    Energy Technology Data Exchange (ETDEWEB)

    Itahashi, T.; Takahisa, K.; Fujiwara, M.; Toki, H.; Ejiri, H. [Osaka Univ., Ibaraki (Japan). Research Center for Nuclear Physics; Ohsumi, H.; Komori, M.

    1997-03-01

    A compact accelerator with high current ion source, low energy beam transport elements and windowless gas target was designed to investigate the thermonuclear reaction cross section. The idea of this project focused on the cross section measurement of the fusion reaction data {sup 3}He+{sup 3}He-{sup 4}He+p+p at 25keV. The system will be installed in Otoh Cosmo Observatory (1270m.w.e.) to get rid of the huge cosmic and environmental background. It consists of NANOGUN ECR ion source, focusing elements made of permanent magnets window less {sup 3}He gas target and/or He{sup 3} plasma target and detector telescopes with low noise and low background. Requirements for these were discussed technically and various ideas were proposed. (author)

  5. Opposing microtubule motors control motility, morphology and cargo segregation during ER-to-Golgi transport

    Directory of Open Access Journals (Sweden)

    Anna K. Brown

    2014-04-01

    Full Text Available We recently demonstrated that dynein and kinesin motors drive multiple aspects of endosomal function in mammalian cells. These functions include driving motility, maintaining morphology (notably through providing longitudinal tension to support vesicle fission, and driving cargo sorting. Microtubule motors drive bidirectional motility during traffic between the endoplasmic reticulum (ER and Golgi. Here, we have examined the role of microtubule motors in transport carrier motility, morphology, and domain organization during ER-to-Golgi transport. We show that, consistent with our findings for endosomal dynamics, microtubule motor function during ER-to-Golgi transport of secretory cargo is required for motility, morphology, and cargo sorting within vesicular tubular carriers en route to the Golgi. Our data are consistent with previous findings that defined roles for dynein-1, kinesin-1 (KIF5B and kinesin-2 in this trafficking step. Our high resolution tracking data identify some intriguing aspects. Depletion of kinesin-1 reduces the number of motile structures seen, which is in line with other findings relating to the role of kinesin-1 in ER export. However, those transport carriers that were produced had a much greater run length suggesting that this motor can act as a brake on anterograde motility. Kinesin-2 depletion did not significantly reduce the number of motile transport carriers but did cause a similar increase in run length. These data suggest that kinesins act as negative regulators of ER-to-Golgi transport. Depletion of dynein not only reduced the number of motile carriers formed but also caused tubulation of carriers similar to that seen for sorting nexin-coated early endosomes. Our data indicated that the previously observed anterograde–retrograde polarity of transport carriers in transit to the Golgi from the ER is maintained by microtubule motor function.

  6. A C-terminal PDZ domain-binding sequence is required for striatal distribution of the dopamine transporter

    DEFF Research Database (Denmark)

    Rickhag, Karl Mattias; Hansen, Freja Herborg; Sørensen, Gunnar;

    2013-01-01

    The dopamine transporter mediates reuptake of dopamine from the synaptic cleft. The cellular mechanisms controlling dopamine transporter levels in striatal nerve terminals remain poorly understood. The dopamine transporters contain a C-terminal PDZ (PSD-95/Discs-large/ZO-1) domain-binding sequenc...

  7. Not used to public transport any longer. A study about transport service based on needs and requirements in Siljansnaes; Man har vant sig av med kollektivtrafik. En studie om transportservice baserad paa behov och vanor i Siljansnaes

    Energy Technology Data Exchange (ETDEWEB)

    Warsen, Lisa

    2000-05-01

    The goal of the transport policy is that every citizen shall have a transport service on a reasonable level. In sparsely populated areas the public transport service is on a low level and many groups without a private car will have small possibilities to travel to different activities. Siljansnaes, with nearly 2000 citizens, is a small region in the community Leksand in Dalarna where the transport problem have been more tangible. The aim of this project has been to create a transport system more based on the needs and requirements. Also the people who lives in Siljansnaes in their leisure times are a part in the project. They are almost as many as the permanent living ones. The result also has been built on the goal of a higher care of the environment. The transport solution contains; village-bus, car-pooling, changed price system for public transport, but also a partnership between the citizens, the community and the regional transport company (Dalatrafik). The project as a process has the goal that the people in Siljansnaes should take part of the solution and the responsibility for the operation of the system.

  8. The ArfGEF GBF-1 Is Required for ER Structure, Secretion and Endocytic Transport in C. elegans.

    Directory of Open Access Journals (Sweden)

    Karin B Ackema

    Full Text Available Small GTPases of the Sar/Arf family are essential to generate transport containers that mediate communication between organelles of the secretory pathway. Guanine nucleotide exchange factor (GEFs activate the small GTPases and help their anchorage in the membrane. Thus, GEFs in a way temporally and spatially control Sar1/Arf1 GTPase activation. We investigated the role of the ArfGEF GBF-1 in C. elegans oocytes and intestinal epithelial cells. GBF-1 localizes to the cis-Golgi and is part of the t-ER-Golgi elements. GBF-1 is required for secretion and Golgi integrity. In addition, gbf-1(RNAi causes the ER reticular structure to become dispersed, without destroying ER exit sites (ERES because the ERES protein SEC-16 was still localized in distinct punctae at t-ER-Golgi units. Moreover, GBF-1 plays a role in receptor-mediated endocytosis in oocytes, without affecting recycling pathways. We find that both the yolk receptor RME-2 and the recycling endosome-associated RAB-11 localize similarly in control and gbf-1(RNAi oocytes. While RAB5-positive early endosomes appear to be less prominent and the RAB-5 levels are reduced by gbf-1(RNAi in the intestine, RAB-7-positive late endosomes were more abundant and formed aggregates and tubular structures. Our data suggest a role for GBF-1 in ER structure and endosomal traffic.

  9. Gli2 and gli3 localize to cilia and require the intraflagellar transport protein polaris for processing and function.

    Directory of Open Access Journals (Sweden)

    2005-10-01

    Full Text Available Intraflagellar transport (IFT proteins are essential for cilia assembly and have recently been associated with a number of developmental processes, such as left-right axis specification and limb and neural tube patterning. Genetic studies indicate that IFT proteins are required for Sonic hedgehog (Shh signaling downstream of the Smoothened and Patched membrane proteins but upstream of the Glioma (Gli transcription factors. However, the role that IFT proteins play in transduction of Shh signaling and the importance of cilia in this process remain unknown. Here we provide insights into the mechanism by which defects in an IFT protein, Tg737/Polaris, affect Shh signaling in the murine limb bud. Our data show that loss of Tg737 results in altered Gli3 processing that abrogates Gli3-mediated repression of Gli1 transcriptional activity. In contrast to the conclusions drawn from genetic analysis, the activity of Gli1 and truncated forms of Gli3 (Gli3R are unaffected in Tg737 mutants at the molecular level, indicating that Tg737/Polaris is differentially involved in specific activities of the Gli proteins. Most important, a negative regulator of Shh signaling, Suppressor of fused, and the three full-length Gli transcription factors localize to the distal tip of cilia in addition to the nucleus. Thus, our data support a model where cilia have a direct role in Gli processing and Shh signal transduction.

  10. Gli2 and Gli3 localize to cilia and require the intraflagellar transport protein polaris for processing and function.

    Directory of Open Access Journals (Sweden)

    Courtney J Haycraft

    2005-10-01

    Full Text Available Intraflagellar transport (IFT proteins are essential for cilia assembly and have recently been associated with a number of developmental processes, such as left-right axis specification and limb and neural tube patterning. Genetic studies indicate that IFT proteins are required for Sonic hedgehog (Shh signaling downstream of the Smoothened and Patched membrane proteins but upstream of the Glioma (Gli transcription factors. However, the role that IFT proteins play in transduction of Shh signaling and the importance of cilia in this process remain unknown. Here we provide insights into the mechanism by which defects in an IFT protein, Tg737/Polaris, affect Shh signaling in the murine limb bud. Our data show that loss of Tg737 results in altered Gli3 processing that abrogates Gli3-mediated repression of Gli1 transcriptional activity. In contrast to the conclusions drawn from genetic analysis, the activity of Gli1 and truncated forms of Gli3 (Gli3R are unaffected in Tg737 mutants at the molecular level, indicating that Tg737/Polaris is differentially involved in specific activities of the Gli proteins. Most important, a negative regulator of Shh signaling, Suppressor of fused, and the three full-length Gli transcription factors localize to the distal tip of cilia in addition to the nucleus. Thus, our data support a model where cilia have a direct role in Gli processing and Shh signal transduction.

  11. Gli2 and Gli3 localize to cilia and require the intraflagellar transport protein polaris for processing and function.

    Science.gov (United States)

    Haycraft, Courtney J; Banizs, Boglarka; Aydin-Son, Yesim; Zhang, Qihong; Michaud, Edward J; Yoder, Bradley K

    2005-10-01

    Intraflagellar transport (IFT) proteins are essential for cilia assembly and have recently been associated with a number of developmental processes, such as left-right axis specification and limb and neural tube patterning. Genetic studies indicate that IFT proteins are required for Sonic hedgehog (Shh) signaling downstream of the Smoothened and Patched membrane proteins but upstream of the Glioma (Gli) transcription factors. However, the role that IFT proteins play in transduction of Shh signaling and the importance of cilia in this process remain unknown. Here we provide insights into the mechanism by which defects in an IFT protein, Tg737/Polaris, affect Shh signaling in the murine limb bud. Our data show that loss of Tg737 results in altered Gli3 processing that abrogates Gli3-mediated repression of Gli1 transcriptional activity. In contrast to the conclusions drawn from genetic analysis, the activity of Gli1 and truncated forms of Gli3 (Gli3R) are unaffected in Tg737 mutants at the molecular level, indicating that Tg737/Polaris is differentially involved in specific activities of the Gli proteins. Most important, a negative regulator of Shh signaling, Suppressor of fused, and the three full-length Gli transcription factors localize to the distal tip of cilia in addition to the nucleus. Thus, our data support a model where cilia have a direct role in Gli processing and Shh signal transduction. PMID:16254602

  12. Gli2 and Gli3 Localize to Cilia and Require the Intraflagellar Transport Protein Polaris for Processing and Function

    Energy Technology Data Exchange (ETDEWEB)

    Michaud III, Edward J [ORNL; Haycraft, Courtney J [University of Alabama, Birmingham; Aydin Son, Yesim [University of Tennessee, Knoxville (UTK); Zhang, Qihong [University of Alabama, Birmingham; Yoder, Bradley [University of Alabama, Birmingham

    2005-01-01

    Intraflagellar transport (IFT) proteins are essential for cilia assembly and have recently been associated with a number of developmental processes, such as left-right axis specification and limb and neural tube patterning. Genetic studies indicate that IFT proteins are required for Sonic hedgehog (Shh)signaling downstream of the Smoothened and Patched membrane proteins but upstream of the Glioma (Gli) transcription factors. However, the role that IFT proteins play in transduction of Shh signaling and the importance of cilia in this process remain unknown. Here we provide insights into the mechanism by which defects in an IFT protein, Tg737/Polaris, affect Shh signaling in the murine limb bud. Our data show that loss of Tg737 results in altered Gli3 processing that abrogates Gli3-mediated repression of Gli1 transcriptional activity. In contrast to the conclusions drawn from genetic analysis, the activity of Gli1 and truncated forms of Gli3 (Gli3R) are unaffected in Tg737 mutants at the molecular level, indicating that Tg737/Polaris is differentially involved in specific activities of the Gli proteins. Most important, a negative regulator of Shh signaling, Suppressor of fused, and the three full-length Gli transcription factors localize to the distal tip of cilia in addition to the nucleus. Thus, our data support a model where cilia have a direct role in Gli processing and Shh signal transduction.

  13. A Quantitative bgl Operon Model for E. coli Requires BglF Conformational Change for Sugar Transport

    Science.gov (United States)

    Chopra, Paras; Bender, Andreas

    The bgl operon is responsible for the metabolism of β-glucoside sugars such as salicin or arbutin in E. coli. Its regulatory system involves both positive and negative feedback mechanisms and it can be assumed to be more complex than that of the more closely studied lac and trp operons. We have developed a quantitative model for the regulation of the bgl operon which is subject to in silico experiments investigating its behavior under different hypothetical conditions. Upon administration of 5mM salicin as an inducer our model shows 80-fold induction, which compares well with the 60-fold induction measured experimentally. Under practical conditions 5-10mM inducer are employed, which is in line with the minimum inducer concentration of 1mM required by our model. The necessity of BglF conformational change for sugar transport has been hypothesized previously, and in line with those hypotheses our model shows only minor induction if conformational change is not allowed. Overall, this first quantitative model for the bgl operon gives reasonable predictions that are close to experimental results (where measured). It will be further refined as values of the parameters are determined experimentally. The model was developed in Systems Biology Markup Language (SBML) and it is available from the authors and from the Biomodels repository [www.ebi.ac.uk/biomodels].

  14. 41 CFR 102-118.25 - Does GSA still require my agency to submit its overall transportation policies for approval?

    Science.gov (United States)

    2010-07-01

    ... Public Contracts and Property Management Federal Property Management Regulations System (Continued) FEDERAL MANAGEMENT REGULATION TRANSPORTATION 118-TRANSPORTATION PAYMENT AND AUDIT General Introduction... 41 Public Contracts and Property Management 3 2010-07-01 2010-07-01 false Does GSA still...

  15. Analysis of transport logistics and routing requirements for radioactive waste management systems with respect to a minimum power scenario

    International Nuclear Information System (INIS)

    This report assesses the transport logistics associated with disposal of intermediate-level radioactive waste, as generated by CEGB, SSEB, UKAEA and BNFL, in accordance with a 'Minimum Power Scenario'. Transport by road and rail is analysed, as in previous reports; use of coastal shipping however has not been included but has been replaced with a combined road/rail option. (author)

  16. Dynamics of mitochondrial transport in axons

    Directory of Open Access Journals (Sweden)

    Robert Francis Niescier

    2016-05-01

    Full Text Available The polarized structure and long neurites of neurons pose a unique challenge for proper mitochondrial distribution. It is widely accepted that mitochondria move from the cell body to axon ends and vice versa; however, we have found that mitochondria originating from the axon ends moving in the retrograde direction never reach to the cell body, and only a limited number of mitochondria moving in the anterograde direction from the cell body arrive at the axon ends of mouse hippocampal neurons. Furthermore, we have derived a mathematical formula using the Fokker-Planck equation to characterize features of mitochondrial transport, and the equation could determine altered mitochondrial transport in axons overexpressing parkin. Our analysis will provide new insights into the dynamics of mitochondrial transport in axons of normal and unhealthy neurons.

  17. Dynamics of Mitochondrial Transport in Axons.

    Science.gov (United States)

    Niescier, Robert F; Kwak, Sang Kyu; Joo, Se Hun; Chang, Karen T; Min, Kyung-Tai

    2016-01-01

    The polarized structure and long neurites of neurons pose a unique challenge for proper mitochondrial distribution. It is widely accepted that mitochondria move from the cell body to axon ends and vice versa; however, we have found that mitochondria originating from the axon ends moving in the retrograde direction never reach to the cell body, and only a limited number of mitochondria moving in the anterograde direction from the cell body arrive at the axon ends of mouse hippocampal neurons. Furthermore, we have derived a mathematical formula using the Fokker-Planck equation to characterize features of mitochondrial transport, and the equation could determine altered mitochondrial transport in axons overexpressing parkin. Our analysis will provide new insights into the dynamics of mitochondrial transport in axons of normal and unhealthy neurons. PMID:27242435

  18. Report of consultants to the IAEA on requirements for the safe transport of low hazard radioactive materials

    International Nuclear Information System (INIS)

    In the present paper changes are recommmended to certain definitions given in the 2nd draft revision of the IAEA 'regulations for the safe transport of radioactive materials (safety series no. 6)'. (orig./RW)

  19. A novel multiprotein complex is required to generate the prechylomicron transport vesicle from intestinal ER[S

    OpenAIRE

    Siddiqi, Shahzad; Saleem, Umair; Abumrad, Nada A.; Davidson, Nicholas O.; Storch, Judith; Siddiqi, Shadab A.; Mansbach, Charles M.

    2010-01-01

    Dietary lipid absorption is dependent on chylomicron production whose rate-limiting step across the intestinal absorptive cell is the exit of chylomicrons from the endoplasmic reticulum (ER) in its ER-to-Golgi transport vesicle, the prechylomicron transport vesicle (PCTV). This study addresses the composition of the budding complex for PCTV. Immunoprecipitation (IP) studies from rat intestinal ER solubilized in Triton X-100 suggested that vesicle-associated membrane protein 7 (VAMP7), apolipo...

  20. The carboxy-terminus of VirE2 from Agrobacterium tumefaciens is required for its transport to host cells by the virB-encoded type IV transport system.

    Science.gov (United States)

    Simone, M; McCullen, C A; Stahl, L E; Binns, A N

    2001-09-01

    Agrobacterium tumefaciens transfers DNA from the resident 'tumour-inducing' (Ti) plasmid into plant cells, where it can be stably integrated into the plant genome, ultimately resulting in crown gall tumour formation. The mobilized DNA molecule is a single-stranded intermediate with VirD2 covalently bound to its 5' end. Successful transport of the transferred DNA (T-DNA) and integration of the DNA into the genome requires that additional proteins be transported to the plant as well, including the single-stranded (ss)DNA-binding protein, VirE2. The transport of these two different substrates occurs as a result of the activities of a type IV secretion system encoded by the virB operon. Although the substrates have been identified, the mechanism of their transport remains unknown. In the experiments described here, a region in one of these substrates, VirE2, necessary for transport is identified. The addition of a C-terminal FLAG epitope tag to VirE2, or the deletion of its C-terminal 18 amino acids, renders it non-functional in A. tumefaciens. However, transgenic plants expressing either of these virE2 genes respond to virE2 mutants of A. tumefaciens by forming wild-type tumours. These results indicate that this region of VirE2 is necessary for the protein to be transported into the plant cells, but is not necessary for its function within the plant. Additionally, these studies demonstrate that mutant forms of VirE2 lacking this region do not disrupt the activities of the VirB transporter and support the hypothesis that VirE2 and the VirD2 T-strand are transported independently, even when they co-exist in the same cell. PMID:11580834

  1. Transportation of radioactive and hazardous materials: a summary of state and local legislative requirements for the period ending December 31, 1984

    International Nuclear Information System (INIS)

    This report summarizes 513 adopted US state and local laws that impact the transportation of radioactive materials. The report was generated from legislative information contained in the Legislative Data Base (LDB), a comprehensive interactive database developed at the Oak Ridge National Laboratory for the US Department of Energy. The annotated citations alphabetically by state, with state and local bills listed separately and sorted by date of adoption. Each citation contains the following information: locale (geographical areas and political jurisdictions affected by the action), bill number, bill title, bill sponsor, history of bill status, comments, and abstract. Six indexes are provided to assist the reader in locating legislation of interest: locale index, bill number index, title word index (permuted), sponsor index, transport restriction index (type of transportation restriction specified, e.g., escort, notify, permit, ban), transport mode index (mode of transportation specified, e.g., truck, rail, barge), and keyword index. This report updates the information contained in Transportation of Radioactive and Hazardous Materials: A Summary of State and Local Legislative Requirements for the Period ending September 30, 1983, ORNL/TM-8860 (TTC-0485), published in June 1984

  2. Biochemical analysis of PIFTC3, the Trypanosoma brucei orthologue of nematode DYF-13, reveals interactions with established and putative intraflagellar transport components.

    Science.gov (United States)

    Franklin, Joseph B; Ullu, Elisabetta

    2010-10-01

    DYF-13, originally identified in Caenorhabditis elegans within a collection of dye-filling chemosensory mutants, is one of several proteins that have been classified as putatively involved in intraflagellar transport (IFT), the bidirectional movement of protein complexes along cilia and flagella and specifically in anterograde IFT. Although genetic studies have highlighted a fundamental role of DYF-13 in nematode sensory cilium and trypanosome flagellum biogenesis, biochemical studies on DYF-13 have lagged behind. Here, we show that in Trypanosoma brucei the orthologue to DYF-13, PIFTC3, participates in a macromolecular complex of approximately 660 kDa. Mass spectroscopy of affinity-purified PIFTC3 revealed several components of IFT complex B as well as orthologues of putative IFT factors DYF-1, DYF-3, DYF-11/Elipsa and IFTA-2. DYF-11 was further analysed and shown to be concentrated near the basal bodies and in the flagellum, and to be required for flagellum elongation. In addition, by coimmunoprecipitation we detected an interaction between DYF-13 and IFT122, a component of IFT complex A, which is required for retrograde transport. Thus, our biochemical analysis supports the model, proposed by genetic analysis in C. elegans, that the trypanosome orthologue of DYF-13 plays a central role in the IFT mechanism. PMID:20923419

  3. 军用危险货物运输对铁路车辆的需求%Requirements of Railway Wagons on Transportation of Military Dangerous Goods

    Institute of Scientific and Technical Information of China (English)

    程虹; 于洋; 季锋; 曾已泷

    2012-01-01

    To counter the main problem of the military dangerous goods railway transportation combined with the trend of our country' s railway technical development. This paper analyzed the characteristics of railway transportations of military dangerous goods and the requirements of railway wagons. It is helpful to provide technical support to resolve the problems of railway wagons in military dangerous goods' transportation.%针对当前军用危险货物铁路运输中存在的主要问题,结合我国铁路技术装备发展趋势,研究分析军用危险货物铁路运输特点及对铁路车辆的要求,为解决军用危险货物铁路运输车辆保障难题提供技术支持。

  4. The MRP2/cMOAT transporter and arsenic-glutathione complex formation are required for biliary excretion of arsenic.

    Science.gov (United States)

    Kala, S V; Neely, M W; Kala, G; Prater, C I; Atwood, D W; Rice, J S; Lieberman, M W

    2000-10-27

    Worldwide, millions of people are exposed to arsenic in drinking water that exceeds the World Health Organization standard of 10 microg/liter by as much as 50-300-fold, yet little is known about the molecular basis for arsenic excretion. Here we show that transport of arsenic into bile depends on the MRP2/cMOAT transporter and that glutathione is obligatory for such transport. Using reversed phase liquid chromatography/mass spectrometry, we demonstrate that two arsenic-glutathione complexes not previously identified in vivo, arsenic triglutathione and methylarsenic diglutathione, account for most of the arsenic in the bile. The structure of the compounds was also confirmed by nuclear magnetic resonance spectroscopy. Our findings may help explain the increased susceptibility of malnourished human populations to arsenic. PMID:10938093

  5. The transport sectors potential contribution to the flexibility in the power sector required by large-scale wind power integration

    DEFF Research Database (Denmark)

    Nørgaard, Per; Lund, Henrik; Mathiesen, Brian Vad

    2007-01-01

    -scale integration of renewable energy in the power system – in specific wind power. In the plan, 20 % of the road transport is based on electricity and 20 % on bio- fuels. This, together with other initiatives allows for up to 55-60 % wind power penetration in the power system. A fleet of 0.5 mio electrical......In 2006, the Danish Society of Engineers developed a visionary plan for the Danish energy system in 2030. The paper presents and qualifies selected part of the analyses, illustrating the transport sectors potential to contribute to the flexibility in the power sector, necessary for large...

  6. Transport of Radioactive Materials

    International Nuclear Information System (INIS)

    This address overviews the following aspects: concepts on transport of radioactive materials, quantities used to limit the transport, packages, types of packages, labeling, index transport calculation, tags, labeling, vehicle's requirements and documents required to authorize transportation. These requirements are considered in the regulation of transport of radioactive material that is in drafting step

  7. Identification of different types of spinal afferent nerve endings that encode noxious and innocuous stimuli in the large intestine using a novel anterograde tracing technique.

    Directory of Open Access Journals (Sweden)

    Nick J Spencer

    Full Text Available In mammals, sensory stimuli in visceral organs, including those that underlie pain perception, are detected by spinal afferent neurons, whose cell bodies lie in dorsal root ganglia (DRG. One of the major challenges in visceral organs has been how to identify the different types of nerve endings of spinal afferents that transduce sensory stimuli into action potentials. The reason why spinal afferent nerve endings have been so challenging to identify is because no techniques have been available, until now, that can selectively label only spinal afferents, in high resolution. We have utilized an anterograde tracing technique, recently developed in our laboratory, which facilitates selective labeling of only spinal afferent axons and their nerve endings in visceral organs. Mice were anesthetized, lumbosacral DRGs surgically exposed, then injected with dextran-amine. Seven days post-surgery, the large intestine was removed. The characteristics of thirteen types of spinal afferent nerve endings were identified in detail. The greatest proportion of nerve endings was in submucosa (32%, circular muscle (25% and myenteric ganglia (22%. Two morphologically distinct classes innervated myenteric ganglia. These were most commonly a novel class of intraganglionic varicose endings (IGVEs and occasionally rectal intraganglionic laminar endings (rIGLEs. Three distinct classes of varicose nerve endings were found to innervate the submucosa and circular muscle, while one class innervated internodal strands, blood vessels, crypts of lieberkuhn, the mucosa and the longitudinal muscle. Distinct populations of sensory endings were CGRP-positive. We present the first complete characterization of the different types of spinal afferent nerve endings in a mammalian visceral organ. The findings reveal an unexpectedly complex array of different types of primary afferent endings that innervate specific layers of the large intestine. Some of the novel classes of nerve endings

  8. Identification of Different Types of Spinal Afferent Nerve Endings That Encode Noxious and Innocuous Stimuli in the Large Intestine Using a Novel Anterograde Tracing Technique

    Science.gov (United States)

    Spencer, Nick J.; Kyloh, Melinda; Duffield, Michael

    2014-01-01

    In mammals, sensory stimuli in visceral organs, including those that underlie pain perception, are detected by spinal afferent neurons, whose cell bodies lie in dorsal root ganglia (DRG). One of the major challenges in visceral organs has been how to identify the different types of nerve endings of spinal afferents that transduce sensory stimuli into action potentials. The reason why spinal afferent nerve endings have been so challenging to identify is because no techniques have been available, until now, that can selectively label only spinal afferents, in high resolution. We have utilized an anterograde tracing technique, recently developed in our laboratory, which facilitates selective labeling of only spinal afferent axons and their nerve endings in visceral organs. Mice were anesthetized, lumbosacral DRGs surgically exposed, then injected with dextran-amine. Seven days post-surgery, the large intestine was removed. The characteristics of thirteen types of spinal afferent nerve endings were identified in detail. The greatest proportion of nerve endings was in submucosa (32%), circular muscle (25%) and myenteric ganglia (22%). Two morphologically distinct classes innervated myenteric ganglia. These were most commonly a novel class of intraganglionic varicose endings (IGVEs) and occasionally rectal intraganglionic laminar endings (rIGLEs). Three distinct classes of varicose nerve endings were found to innervate the submucosa and circular muscle, while one class innervated internodal strands, blood vessels, crypts of lieberkuhn, the mucosa and the longitudinal muscle. Distinct populations of sensory endings were CGRP-positive. We present the first complete characterization of the different types of spinal afferent nerve endings in a mammalian visceral organ. The findings reveal an unexpectedly complex array of different types of primary afferent endings that innervate specific layers of the large intestine. Some of the novel classes of nerve endings identified

  9. Serotonin transporter is not required for the development of severe pulmonary hypertension in the Sugen hypoxia rat model

    NARCIS (Netherlands)

    de Raaf, Michiel Alexander; Kroeze, Yvet; Middelman, Anthonieke; de Man, Frances S.; de Jong, Helma; Vonk-Noordegraaf, Anton; de Korte, Chris; Voelkel, Norbert F.; Homberg, Judith; Bogaard, Harm Jan

    2015-01-01

    Increased serotonin serum levels have been proposed to play a key role in pulmonary arterial hypertension (PAH) by regulating vessel tone and vascular smooth muscle cell proliferation. An intact serotonin system, which critically depends on a normal function of the serotonin transporter (SERT), is r

  10. Zebrafish ("Danio rerio") endomembrane antiporter similar to a yeast cation/H(+) transporter is required for neural crest development

    Science.gov (United States)

    CAtion/H (+) eXchangers (CAXs) are integral membrane proteins that transport Ca (2+) or other cations by exchange with protons. While several yeast and plant CAX proteins have been characterized, no functional analysis of a vertebrate CAX homologue has yet been reported. In this study, we further ch...

  11. The DotA protein from Legionella pneumophila is secreted by a novel process that requires the Dot/Icm transporter

    OpenAIRE

    Nagai, Hiroki; Roy, Craig R.

    2001-01-01

    Legionella pneumophila requires the dot/icm genes to create an organelle inside eukaryotic host cells that will support bacterial replication. The dot/icm genes are predicted to encode a type IV-related secretion apparatus. However, no proteins have been identified that require the dot/icm genes for secretion. In this study we show that the DotA protein, which was previously found to be a polytopic membrane protein, is secreted by the Dot/Icm transporter into culture supernatants. Secreted Do...

  12. Dispositivo de tração do membro superior para osteossínteses intramedulares bloqueadas anterógradas de fraturas diafisárias de úmero Upper limb traction device for anterograde intramedullary locked nail of humeral shaft fractures

    Directory of Open Access Journals (Sweden)

    Mário Chaves Corrêa

    2010-06-01

    surgically, usually by means of intramedullary locked nail. Some comminuted and/or very deviated shaft fractures can represent a real technical challenge. The fracture table, which allows for the vertical, horizontal and rotational instrumental stabilization of the limb, greatly facilitates reduction and implant placement maneuvers and are widely used by orthopedic surgeons. Humeral shaft fractures are mostly treated nonsurgically. However, some whose indications are well defined in literature require surgical treatment. They can be fixed by plates, or by anterograde or retrograde intramedullary nail. In the humerus, limb fracture reduction and stabilization maneuvers for implantation of intramedullary nails are done manually, usually by 2 assistants. Because they are subject to muscle fatigue, this option may be less efficient. The aim of this paper is to present an external traction device for use in anterograde intramedullary fixation of humerus shaft fractures that allows vertical, horizontal and rotational stabilization of the upper limb similarly to that used in the lower limbs. The device is portable, of simple construction, and can be installed on any operating table equipped with side rails. It was used in the surgical treatment of 29 humeral shaft fractures with anterograde locked intramedullary nail. Our experience was extremely positive. We had no complications related to its use and we believe it to have facilitated the surgical procedures in a remarkable way.

  13. Customer satisfaction techniques applied to the transport of nuclear fuels to meet ISO 9001:2000 requirements

    International Nuclear Information System (INIS)

    Understanding the needs of potential and existing customers is seen as essential to the continuing success of the nuclear fuel transport business. A recognised process utilised for this is the recording and measurement of customer satisfaction. This paper looks at what customer satisfaction means to BNFL International Transport in terms of, understanding what the customers needs are, supplying those needs or proposing appropriate alternatives, delivering on contractual obligations, in terms of the product or service being supplied to cost and time, listening to the changing needs of customers should this occur and monitoring the perception of customers in terms of performance. Within BNFL as a whole customer satisfaction is managed through the application of a Management Framework. Within this framework are set a number of values, these are focused on acknowledging social and environmental responsibilities while satisfying the needs of customers. Customer satisfaction is a key aspect of this philosophy

  14. A glial variant of the vesicular monoamine transporter is required to store histamine in the Drosophila visual system.

    Directory of Open Access Journals (Sweden)

    Rafael Romero-Calderón

    2008-11-01

    Full Text Available Unlike other monoamine neurotransmitters, the mechanism by which the brain's histamine content is regulated remains unclear. In mammals, vesicular monoamine transporters (VMATs are expressed exclusively in neurons and mediate the storage of histamine and other monoamines. We have studied the visual system of Drosophila melanogaster in which histamine is the primary neurotransmitter released from photoreceptor cells. We report here that a novel mRNA splice variant of Drosophila VMAT (DVMAT-B is expressed not in neurons but rather in a small subset of glia in the lamina of the fly's optic lobe. Histamine contents are reduced by mutation of dVMAT, but can be partially restored by specifically expressing DVMAT-B in glia. Our results suggest a novel role for a monoamine transporter in glia that may be relevant to histamine homeostasis in other systems.

  15. CFP, the putative cercosporin transporter of Cercospora kikuchii, is required for wild type cercosporin production, resistance, and virulence on soybean.

    Science.gov (United States)

    Callahan, T M; Rose, M S; Meade, M J; Ehrenshaft, M; Upchurch, R G

    1999-10-01

    Many species of the fungal genus Cercospora, including the soybean pathogen C. kikuchii, produce the phytotoxic polyketide cercosporin. Cercosporin production is induced by light. Previously, we identified several cDNA clones of mRNA transcripts that exhibited light-enhanced accumulation in C. kikuchii. Targeted disruption of the genomic copy of one of these, now designated CFP (cercosporin facilitator protein), results in a drastic reduction in cercosporin production, greatly reduced virulence of the fungus to soybean, and increased sensitivity to exogenous cercosporin. Sequence analysis of CFP reveals an 1,821-bp open reading frame encoding a 65.4-kDa protein similar to several members of the major facilitator superfamily (MFS) of integral membrane transporter proteins known to confer resistance to various antibiotics and toxins in fungi and bacteria. We propose that CFP encodes a cercosporin transporter that contributes resistance to cercosporin by actively exporting cercosporin, thus maintaining low cellular concentrations of the toxin.

  16. Customer satisfaction techniques applied to the transport of nuclear fuels to meet ISO 9001:2000 requirements

    Energy Technology Data Exchange (ETDEWEB)

    Fisher, G.K. [BNFL (United Kingdom)

    2004-07-01

    Understanding the needs of potential and existing customers is seen as essential to the continuing success of the nuclear fuel transport business. A recognised process utilised for this is the recording and measurement of customer satisfaction. This paper looks at what customer satisfaction means to BNFL International Transport in terms of, understanding what the customers needs are, supplying those needs or proposing appropriate alternatives, delivering on contractual obligations, in terms of the product or service being supplied to cost and time, listening to the changing needs of customers should this occur and monitoring the perception of customers in terms of performance. Within BNFL as a whole customer satisfaction is managed through the application of a Management Framework. Within this framework are set a number of values, these are focused on acknowledging social and environmental responsibilities while satisfying the needs of customers. Customer satisfaction is a key aspect of this philosophy.

  17. Implementing the connected e-bike: challenges and requirements of an IoT application for urban transportation

    OpenAIRE

    Flüchter, Kristina; Wortmann, Felix

    2014-01-01

    As local transportation systems are facing enormous challenges in consequence of extensive urban growth, technological advancements are creating unprecedented opportunities as the Internet is extending into the real world, connecting physical items to the virtual world and creating an Internet of Things (IoT). While many activities are exploring corresponding applications, e.g. connecting bicycles to the Internet, widespread economic success of a truly connected device is still outstanding. F...

  18. A C-terminal PDZ domain binding sequence is required for striatal distribution of the dopamine transporter

    OpenAIRE

    Rickhag, Mattias; Hansen, Freja Herborg; Sørensen, Gunnar; Strandfelt, Kristine Nørgaard; Andresen, Bjørn; Gotfryd, Kamil; Madsen, Kenneth L; Vestergaard-Klewe, Ib; Ammendrup-Johnsen, Ina; Eriksen, Jacob; Füchtbauer, Ernst-Martin; Gomeza, Jesus; Woldbye, David P.D.; Wörtwein, Gitta; Gether, Ulrik

    2013-01-01

    The dopamine transporter (DAT) mediates reuptake of dopamine from the synaptic cleft. The cellular mechanisms controlling DAT levels in striatal nerve terminals remain poorly understood. DAT contains a C-terminal PDZ (PSD-95/Discs-large/ZO-1) domain binding sequence believed to bind synaptic scaffolding proteins, but its functional significance is uncertain. Here we demonstrate that two different DAT knock-in mice with disrupted PDZ-binding motifs (DAT-AAA and DAT+Ala) are characterized by dr...

  19. An ABC transporter B family protein, ABCB19, is required for cytoplasmic streaming and gravitropism of the inflorescence stems.

    Science.gov (United States)

    Okamoto, Keishi; Ueda, Haruko; Shimada, Tomoo; Tamura, Kentaro; Koumoto, Yasuko; Tasaka, Masao; Morita, Miyo Terao; Hara-Nishimura, Ikuko

    2016-01-01

    A significant feature of plant cells is the extensive motility of organelles and the cytosol, which was originally defined as cytoplasmic streaming. We suggested previously that a three-way interaction between plant-specific motor proteins myosin XIs, actin filaments, and the endoplasmic reticulum (ER) was responsible for cytoplasmic streaming. (1) Currently, however, there are no reports of molecular components for cytoplasmic streaming other than the actin-myosin-cytoskeleton and ER-related proteins. In the present study, we found that elongated cells of inflorescence stems of Arabidopsis thaliana exhibit vigorous cytoplasmic streaming. Statistical analysis showed that the maximal velocity of plastid movements is 7.26 µm/s, which is much faster than the previously reported velocities of organelles. Surprisingly, the maximal velocity of streaming in the inflorescence stem cells was significantly reduced to 1.11 µm/s in an Arabidopsis mutant, abcb19-101, which lacks ATP BINDING CASSETTE SUBFAMILY B19 (ABCB19) that mediates the polar transport of the phytohormone auxin together with PIN-FORMED (PIN) proteins. Polar auxin transport establishes the auxin concentration gradient essential for plant development and tropisms. Deficiency of ABCB19 activity eventually caused enhanced gravitropic responses of the inflorescence stems and abnormally flexed inflorescence stems. These results suggest that ABCB19-mediated auxin transport plays a role not only in tropism regulation, but also in cytoplasmic streaming.

  20. Amyloid-Beta Induced Changes in Vesicular Transport of BDNF in Hippocampal Neurons

    Directory of Open Access Journals (Sweden)

    Bianca Seifert

    2016-01-01

    Full Text Available The neurotrophin brain derived neurotrophic factor (BDNF is an important growth factor in the CNS. Deficits in transport of this secretory protein could underlie neurodegenerative diseases. Investigation of disease-related changes in BDNF transport might provide insights into the cellular mechanism underlying, for example, Alzheimer’s disease (AD. To analyze the role of BDNF transport in AD, live cell imaging of fluorescently labeled BDNF was performed in hippocampal neurons of different AD model systems. BDNF and APP colocalized with low incidence in vesicular structures. Anterograde as well as retrograde transport of BDNF vesicles was reduced and these effects were mediated by factors released from hippocampal neurons into the extracellular medium. Transport of BDNF was altered at a very early time point after onset of human APP expression or after acute amyloid-beta(1-42 treatment, while the activity-dependent release of BDNF remained unaffected. Taken together, extracellular cleavage products of APP induced rapid changes in anterograde and retrograde transport of BDNF-containing vesicles while release of BDNF was unaffected by transgenic expression of mutated APP. These early transport deficits might lead to permanently impaired brain functions in the adult brain.

  1. SEPS mission and system integration/interface requirements for the space transportation system. [Solar Electric Propulsion System

    Science.gov (United States)

    Cork, M. J.; Barnett, P. M.; Shaffer, J., Jr.; Doran, B. J.

    1979-01-01

    Earth escape mission requirements on Solar Electric Propulsion System (SEPS), and the interface definition and planned integration between SEPS, user spacecraft, and other elements of the STS. Emphasis is placed on the Comet rendezvous mission, scheduled to be the first SEPS user. Interactive SEPS interface characteristics with spacecraft and mission, as well as the multiple organizations and inter-related development schedules required to integrate the SEPS with spacecraft and STS, require early attention to definition of interfaces in order to assure a successful path to the first SEPS launch in July 1985

  2. Biochemical analysis of PIFTC3, the Trypanosoma brucei ortholog of nematode DYF-13, reveals interactions with established and putative intraflagellar transport components

    OpenAIRE

    Franklin, Joseph B.; Ullu, Elisabetta

    2010-01-01

    DYF-13, originally identified in C. elegans within a collection of dye-filling chemosensory mutants, is one of several proteins that have been classified as putatively involved in intraflagellar transport (IFT), the bidirectional movement of protein complexes along cilia and flagella, and specifically in anterograde IFT. Although genetic studies have highlighted a fundamental role of DYF-13 in nematode sensory cilium and trypanosome flagellum biogenesis, biochemical studies on DYF-13 have lag...

  3. The ABCG transporter PEC1/ABCG32 is required for the formation of the developing leaf cuticle in Arabidopsis.

    Science.gov (United States)

    Fabre, Guillaume; Garroum, Imène; Mazurek, Sylwester; Daraspe, Jean; Mucciolo, Antonio; Sankar, Martial; Humbel, Bruno M; Nawrath, Christiane

    2016-01-01

    The cuticle is an essential diffusion barrier on aerial surfaces of land plants whose structural component is the polyester cutin. The PERMEABLE CUTICLE1/ABCG32 (PEC1) transporter is involved in plant cuticle formation in Arabidopsis. The gpat6 pec1 and gpat4 gapt8 pec1 double and triple mutants are characterized. Their PEC1-specific contributions to aliphatic cutin composition and cuticle formation during plant development are revealed by gas chromatography/mass spectrometry and Fourier-transform infrared spectroscopy. The composition of cutin changes during rosette leaf expansion in Arabidopsis. C16:0 monomers are in higher abundance in expanding than in fully expanded leaves. The atypical cutin monomer C18:2 dicarboxylic acid is more prominent in fully expanded leaves. Findings point to differences in the regulation of several pathways of cutin precursor synthesis. PEC1 plays an essential role during expansion of the rosette leaf cuticle. The reduction of C16 monomers in the pec1 mutant during leaf expansion is unlikely to cause permeability of the leaf cuticle because the gpat6 mutant with even fewer C16:0 monomers forms a functional rosette leaf cuticle at all stages of development. PEC1/ABCG32 transport activity affects cutin composition and cuticle structure in a specific and non-redundant fashion.

  4. Pravastatin transport across the hepatocyte canalicular membrane requires both ATP and a transmembrane pH gradient.

    Science.gov (United States)

    Adachi, Y; Okuyama, Y; Miya, H; Matsusita, H; Kitano, M; Kamisako, T; Yamamoto, T

    1996-06-01

    Hepatic excretion of non-bile acid organic anions is reported to be ATP-dependent and a defect of this transport has been reported in congenitally jaundiced rats, animal models of human Dubin-Johnson syndrome. To investigate the effect of the transmembrane pH gradient on hepatocyte canalicular membrane transport of ATP-dependent organic anions, uptake of pravastatin, a 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase-inhibiting organic anion, by hepatocyte canalicular membrane vesicles was observed in the presence or absence of transmembrane pH gradients. Uptake was assessed by a rapid filtration technique. ATP-dependent pravastatin uptake was stimulated in the presence of a transmembrane pH gradient (in > out) in Sprague-Dawley (SD) rats. Uptake was dependent on both pravastatin and ATP concentrations and showed saturation kinetics. After intravenous injection of [14C]-pravastatin (0.3 mumol), 81% of the dose was excreted in the bile within 35 min in SD rats, whereas only 20% was excreted in the bile in Eisai hyperbilirubinuria rats. ATP and the pH gradient also co-stimulated the uptake of pravastatin in Eisai hyperbilirubinuria rats, although the K(m) was much higher and Vmax was much lower than corresponding values in SD rats. This coincided well with the marked reduction in vivo biliary excretion of pravastatin in jaundiced rats.

  5. Requirements for design of accelerator, beam transport, and target in a study of thermonuclear reaction cross section

    Energy Technology Data Exchange (ETDEWEB)

    Itahashi, T.; Takahisa, K.; Ohsumi, H.; Komori, M.; Fujiwara, M.; Toki, H. [Osaka Univ., Suita (Japan)

    1997-02-01

    The process of pp-de{sup +}{nu} is the basic fusion reaction for hydrogen burning in the sun and the prime reaction in chain producing photons and neutrinos. There are many works of the theoretical estimation of the reaction rate in the reaction chain in the sun. The precise measurement of the nutrinos from the sun is one of the most important current physics issues. The rate of the pp-de{sup +}{nu} is too small to be measured in laboratories. The construction of a compact ion accelerator facility with high current, low energy transport and plasma target is planned at the underground laboratory in Otoh Cosmo Observatory of Research Center for Nuclear Physics. The plasma target by using the EBIS type synthesized plasma was proposed as a bare {sup 3}He target. The production of helium ions of each charge state was tested by using the present NEOMAFIOS ECR ion source, and the obtained current is shown. For noncontaminated, high current beam transport, the strong focusing system was introduced. The design of windowless gas target, plasma target, the detection of the energetic reaction particles of protons, digital calorimeter, the couple of ECR ion source and plasma target, and the underground laboratory are reported. (K.I.)

  6. Regulation of Monocarboxylic Acid Transporter 1 Trafficking by the Canonical Wnt/β-Catenin Pathway in Rat Brain Endothelial Cells Requires Cross-talk with Notch Signaling.

    Science.gov (United States)

    Liu, Zejian; Sneve, Mary; Haroldson, Thomas A; Smith, Jeffrey P; Drewes, Lester R

    2016-04-01

    The transport of monocarboxylate fuels such as lactate, pyruvate, and ketone bodies across brain endothelial cells is mediated by monocarboxylic acid transporter 1 (MCT1). Although the canonical Wnt/β-catenin pathway is required for rodent blood-brain barrier development and for the expression of associated nutrient transporters, the role of this pathway in the regulation of brain endothelial MCT1 is unknown. Here we report expression of nine members of the frizzled receptor family by the RBE4 rat brain endothelial cell line. Furthermore, activation of the canonical Wnt/β-catenin pathway in RBE4 cells via nuclear β-catenin signaling with LiCl does not alter brain endothelialMct1mRNA but increases the amount of MCT1 transporter protein. Plasma membrane biotinylation studies and confocal microscopic examination of mCherry-tagged MCT1 indicate that increased transporter results from reduced MCT1 trafficking from the plasma membrane via the endosomal/lysosomal pathway and is facilitated by decreased MCT1 ubiquitination following LiCl treatment. Inhibition of the Notch pathway by the γ-secretase inhibitorN-[N-(3,5-difluorophenacetyl)-l-alanyl]-S-phenylglycinet-butyl ester negated the up-regulation of MCT1 by LiCl, demonstrating a cross-talk between the canonical Wnt/β-catenin and Notch pathways. Our results are important because they show, for the first time, the regulation of MCT1 in cerebrovascular endothelial cells by the multifunctional canonical Wnt/β-catenin and Notch signaling pathways.

  7. Assessment of state and local notification requirements for transportation of radioactive and other hazardous materials. Final report

    International Nuclear Information System (INIS)

    State and local laws requiring notification for shipments of radioactive and other hazardous materials have become increasingly common and controversial during the last decade. Such laws are seen by their proponents as essential for planning and emergency response, while their opponents view them as unnecessary and intrusive. The debate over the value of notification requirements has often been hampered by the lack of information about the extent and nature of these laws. The report is intended to present factual information about notification laws in order to facilitate more informed discussion

  8. Design and characterization of the DC acceleration and transport system required for the FOM 1 MW free electron maser experiment

    Energy Technology Data Exchange (ETDEWEB)

    Caplan, M. [Lawrence Livermore National Lab., CA (United States); Urbanus, W.H.; Geer, C. van der [FOM-Institut voor Plasma Fysica, Nieuwegein (Netherlands)] [and others

    1995-12-31

    A Free Electron Maser (FEM) has been constructed and is soon to be tested at the FOM Institute (Rijnhuizen) Netherlands with the goal of producing 1 MW long pulse to CW microwave output in the range 130 GHz to 250 GHz. The design uses a DC beam system in a depressed collector configuration in order to make the overall wall plug efficiency 50%. The high voltage ({approximately} 2 MeV) power supply provides only the body interception current ({approximately} 30 mA) while the 12 amp beam current is supplied by the 100-200 keV collector supplies. Some of the design features to ensure low interception current, which is critical to long pulse (CW) operation are: (1) DC beam in-line transport and acceleration system, (2) emittance conserving solenoid focusing system, (3) halo suppression techniques at cathode edge, and (4) very low beam fill factor (<20%). A relativistic version of the Herman Optical theory developed for microwave tubes is used to determine current distribution functions everywhere along the beam from the electron gun, through the DC accelerator and transport system to the wiggler. This theory takes into account thermals far out on the gaussian tail which translates into beam current far outside the ideal beam edge. This theory is applied to the FOM beam line design to predict a series of beam envelope contours containing various percentages of total beam current up to 99.9%. Predictions of body interception current due to finite emittance (effective temperature) are presented and compared with measured experimental results.

  9. The ATP-binding Cassette Transporter OsABCG15 is Required for Anther Development and Pollen Fertility in Rice

    Institute of Scientific and Technical Information of China (English)

    Bai-Xiao Niu; Fu-Rong He; Ming He; Ding Ren; Le-Tian Chen; Yao-Guang Liu

    2013-01-01

    Plant male reproductive development is a complex biological process,but the underlying mechanism is not well understood.Here,we characterized a rice (Oryza sativa L.) male sterile mutant.Based on mapbased cloning and sequence analysis,we identified a 1,459-bp deletion in an adenosine triphosphate (ATP)-binding cassette (ABC) transporter gene,OsABCG15,causing abnormal anthers and male sterility.Therefore,we named this mutant osabcg15.Expression analysis showed that OsABCG15 is expressed specifically in developmental anthers from stage 8 (meiosis Ⅱ stage) to stage 10 (late microspore stage).Two genes CYP704B2 and WDA1,involved in the biosynthesis of very-long-chain fatty acids for the establishment of the anther cuticle and pollen exine,were downregulated in osabcg15 mutant,suggesting that OsABCG15 may play a key function in the processes related to sporopollenin biosynthesis or sporopollenin transfer from tapetal cells to anther locules.Consistently,histological analysis showed that osabcg15 mutants developed obvious abnormality in postmeiotic tapetum degeneration,leading to rapid degredation of young microspores.The results suggest that OsABCG15 plays a critical role in exine formation and pollen development,similar to the homologous gene of AtABCG26 in Arabidopsis.This work is helpful to understand the regulatory network in rice anther development.

  10. OsCLT1, a CRT-like transporter 1, is required for glutathione homeostasis and arsenic tolerance in rice.

    Science.gov (United States)

    Yang, Jian; Gao, Ming-Xing; Hu, Han; Ding, Xiao-Meng; Lin, Hong-Wei; Wang, Lei; Xu, Ji-Ming; Mao, Chuan-Zao; Zhao, Fang-Jie; Wu, Zhong-Chang

    2016-07-01

    Arsenic (As) contamination in a paddy environment can cause phytotoxicity and elevated As accumulation in rice (Oryza sativa). The mechanism of As detoxification in rice is still poorly understood. We isolated an arsenate (As(V))-sensitive mutant of rice. Genomic resequencing and complementation identified OsCLT1, encoding a CRT-like transporter, as the causal gene for the mutant phenotype. OsCLT1 is localized to the envelope membrane of plastids. The glutathione and γ-glutamylcysteine contents in roots of Osclt1 and RNA interference lines were decreased markedly compared with the wild-type (WT). The concentrations of phytochelatin PC2 in Osclt1 roots were only 32% and 12% of that in WT after As(V) and As(III) treatments, respectively. OsCLT1 mutation resulted in lower As accumulation in roots but higher As accumulation in shoots when exposed to As(V). Under As(III) treatment, Osclt1 accumulated a lower As concentration in roots but similar As concentration in shoots to WT. Further analysis showed that the reduction of As(V) to As(III) was decreased in Osclt1. Osclt1 was also hypersensitive to cadmium (Cd). These results indicate that OsCLT1 plays an important role in glutathione homeostasis, probably by mediating the export of γ-glutamylcysteine and glutathione from plastids to the cytoplasm, which in turn affects As and Cd detoxification in rice.

  11. A novel high-affinity sucrose transporter is required for virulence of the plant pathogen Ustilago maydis.

    Directory of Open Access Journals (Sweden)

    Ramon Wahl

    2010-02-01

    Full Text Available Plant pathogenic fungi cause massive yield losses and affect both quality and safety of food and feed produced from infected plants. The main objective of plant pathogenic fungi is to get access to the organic carbon sources of their carbon-autotrophic hosts. However, the chemical nature of the carbon source(s and the mode of uptake are largely unknown. Here, we present a novel, plasma membrane-localized sucrose transporter (Srt1 from the corn smut fungus Ustilago maydis and its characterization as a fungal virulence factor. Srt1 has an unusually high substrate affinity, is absolutely sucrose specific, and allows the direct utilization of sucrose at the plant/fungal interface without extracellular hydrolysis and, thus, without the production of extracellular monosaccharides known to elicit plant immune responses. srt1 is expressed exclusively during infection, and its deletion strongly reduces fungal virulence. This emphasizes the central role of this protein both for efficient carbon supply and for avoidance of apoplastic signals potentially recognized by the host.

  12. Functional genomics indicates yeast requires Golgi/ER transport, chromatin remodeling, and DNA repair for low dose DMSO tolerance

    Directory of Open Access Journals (Sweden)

    Brandon David Gaytán

    2013-08-01

    Full Text Available Dimethyl sulfoxide (DMSO is frequently utilized as a solvent in toxicological and pharmaceutical investigations. It is therefore important to establish the cellular and molecular targets of DMSO in order to differentiate its intrinsic effects from those elicited by a compound of interest. We performed a genome-wide functional screen in Saccharomyces cerevisiae to identify deletion mutants exhibiting sensitivity to 1% DMSO, a concentration standard to yeast chemical profiling studies. We report that mutants defective in Golgi/ER transport are sensitive to DMSO, including those lacking components of the conserved oligomeric Golgi (COG complex. Moreover, strains deleted for members of the SWR1 histone exchange complex are hypersensitive to DMSO, with additional chromatin remodeling mutants displaying a range of growth defects. We also identify DNA repair genes important for DMSO tolerance. Finally, we demonstrate that overexpression of histone H2A.Z, which replaces chromatin-associated histone H2A in a SWR1-catalyzed reaction, confers resistance to DMSO. Many yeast genes described in this study have homologs in more complex organisms, and the data provided is applicable to future investigations into the cellular and molecular mechanisms of DMSO toxicity.

  13. Loss of the Coffin-Lowry syndrome-associated gene RSK2 alters ERK activity, synaptic function and axonal transport in Drosophila motoneurons.

    Science.gov (United States)

    Beck, Katherina; Ehmann, Nadine; Andlauer, Till F M; Ljaschenko, Dmitrij; Strecker, Katrin; Fischer, Matthias; Kittel, Robert J; Raabe, Thomas

    2015-11-01

    Plastic changes in synaptic properties are considered as fundamental for adaptive behaviors. Extracellular-signal-regulated kinase (ERK)-mediated signaling has been implicated in regulation of synaptic plasticity. Ribosomal S6 kinase 2 (RSK2) acts as a regulator and downstream effector of ERK. In the brain, RSK2 is predominantly expressed in regions required for learning and memory. Loss-of-function mutations in human RSK2 cause Coffin-Lowry syndrome, which is characterized by severe mental retardation and low IQ scores in affected males. Knockout of RSK2 in mice or the RSK ortholog in Drosophila results in a variety of learning and memory defects. However, overall brain structure in these animals is not affected, leaving open the question of the pathophysiological consequences. Using the fly neuromuscular system as a model for excitatory glutamatergic synapses, we show that removal of RSK function causes distinct defects in motoneurons and at the neuromuscular junction. Based on histochemical and electrophysiological analyses, we conclude that RSK is required for normal synaptic morphology and function. Furthermore, loss of RSK function interferes with ERK signaling at different levels. Elevated ERK activity was evident in the somata of motoneurons, whereas decreased ERK activity was observed in axons and the presynapse. In addition, we uncovered a novel function of RSK in anterograde axonal transport. Our results emphasize the importance of fine-tuning ERK activity in neuronal processes underlying higher brain functions. In this context, RSK acts as a modulator of ERK signaling.

  14. Loss of the Coffin-Lowry syndrome-associated gene RSK2 alters ERK activity, synaptic function and axonal transport in Drosophila motoneurons

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    Katherina Beck

    2015-11-01

    Full Text Available Plastic changes in synaptic properties are considered as fundamental for adaptive behaviors. Extracellular-signal-regulated kinase (ERK-mediated signaling has been implicated in regulation of synaptic plasticity. Ribosomal S6 kinase 2 (RSK2 acts as a regulator and downstream effector of ERK. In the brain, RSK2 is predominantly expressed in regions required for learning and memory. Loss-of-function mutations in human RSK2 cause Coffin-Lowry syndrome, which is characterized by severe mental retardation and low IQ scores in affected males. Knockout of RSK2 in mice or the RSK ortholog in Drosophila results in a variety of learning and memory defects. However, overall brain structure in these animals is not affected, leaving open the question of the pathophysiological consequences. Using the fly neuromuscular system as a model for excitatory glutamatergic synapses, we show that removal of RSK function causes distinct defects in motoneurons and at the neuromuscular junction. Based on histochemical and electrophysiological analyses, we conclude that RSK is required for normal synaptic morphology and function. Furthermore, loss of RSK function interferes with ERK signaling at different levels. Elevated ERK activity was evident in the somata of motoneurons, whereas decreased ERK activity was observed in axons and the presynapse. In addition, we uncovered a novel function of RSK in anterograde axonal transport. Our results emphasize the importance of fine-tuning ERK activity in neuronal processes underlying higher brain functions. In this context, RSK acts as a modulator of ERK signaling.

  15. 右美托咪定对不同年龄患者顺行性遗忘作用的影响%Effects of dexmedetomidine on the anterograde amnesia in patients with different ages

    Institute of Scientific and Technical Information of China (English)

    胡志超; 孔莉; 许鹏程; 李颖; 董晓辉

    2015-01-01

    Objective To study the effects of dexmedetomidine on the anterograde amnesia in patients with different ages.Methods One hundred and twenty ASAⅠ~Ⅱpatients, age from 18 years to 84 years, 73 cases of male and 47 cases of female , who were dministered spinal-epidural anesthesia combined with dexmedetomidine and performed operation on hypogastrium ( except for caesarean section ) .All patients were randomly divided into four groups:group A, group B, group C and group D.Dexmedetomidine(1μg/kg) was infused intravenously for 10 min and the maintenance dose was 0.2 μg・ kg-1・ h-1 .The anterograde amnesia degree of dexmedetomidine was as-sessed 24 h after the operation .Results There was no significant difference in the anterograde amnesia of dexmedeto -midine between group A and group B ( P>0.05 ) , while there were significant differences in group C and group D compared with both group A and group B ( P<0.05 ) .Conclusion Anterograde amnesia occurs after using dexme-detomidine in elderly patients and with aging , the anterograde amnesia becomes more severe .%目的:观察右美托咪定对不同年龄患者顺行性遗忘作用的影响。方法选择美国麻醉医师学会( ASA)Ⅰ~Ⅱ级、年龄18~84岁,在腰硬联合麻醉下复合右美托咪定行下腹部(剖宫产术除外)手术患者120例,其中男73例,女47例。根据患者年龄的不同分为四组:A组(23±4)岁, B组(36±3)岁, C组(65±3)岁,D组(75±5)岁。所有患者均给予右美托咪定负荷量1μg/kg,泵入10 min之后以0.2μg・ kg-1・ h-1剂量维持。术后24 h回访患者并评定右美托咪定的顺行性遗忘作用。结果 A组和B组右美托咪定顺行性遗忘作用无明显不同(P>0.05),而C组与D组较前两组右美托咪定顺行性遗忘作用比较明显(P<0.05),并随年龄的增大明显增加( P<0.05)。结论右美托咪定具有一定的顺行性遗忘作用,且对老年患者更为明显。

  16. Impulse conduction increases mitochondrial transport in adult mammalian peripheral nerves in vivo.

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    Marija Sajic

    2013-12-01

    Full Text Available Matching energy supply and demand is critical in the bioenergetic homeostasis of all cells. This is a special problem in neurons where high levels of energy expenditure may occur at sites remote from the cell body, given the remarkable length of axons and enormous variability of impulse activity over time. Positioning mitochondria at areas with high energy requirements is an essential solution to this problem, but it is not known how this is related to impulse conduction in vivo. Therefore, to study mitochondrial trafficking along resting and electrically active adult axons in vivo, confocal imaging of saphenous nerves in anaesthetised mice was combined with electrical and pharmacological stimulation of myelinated and unmyelinated axons, respectively. We show that low frequency activity induced by electrical stimulation significantly increases anterograde and retrograde mitochondrial traffic in comparison with silent axons. Higher frequency conduction within a physiological range (50 Hz dramatically further increased anterograde, but not retrograde, mitochondrial traffic, by rapidly increasing the number of mobile mitochondria and gradually increasing their velocity. Similarly, topical application of capsaicin to skin innervated by the saphenous nerve increased mitochondrial traffic in both myelinated and unmyelinated axons. In addition, stationary mitochondria in axons conducting at higher frequency become shorter, thus supplying additional mitochondria to the trafficking population, presumably through enhanced fission. Mitochondria recruited to the mobile population do not accumulate near Nodes of Ranvier, but continue to travel anterogradely. This pattern of mitochondrial redistribution suggests that the peripheral terminals of sensory axons represent sites of particularly high metabolic demand during physiological high frequency conduction. As the majority of mitochondrial biogenesis occurs at the cell body, increased anterograde

  17. Phosphatidylinositol and phosphatidic acid transport between the ER and plasma membrane during PLC activation requires the Nir2 protein.

    Science.gov (United States)

    Kim, Yeun Ju; Guzman-Hernandez, Maria Luisa; Wisniewski, Eva; Echeverria, Nicolas; Balla, Tamas

    2016-02-01

    Phospholipase C (PLC)-mediated hydrolysis of the limited pool of plasma membrane (PM) phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P2] requires replenishment from a larger pool of phosphatidylinositol (PtdIns) via sequential phosphorylation by PtdIns 4-kinases and phosphatidylinositol 4-phosphate (PtdIns4P) 5-kinases. Since PtdIns is synthesized in the endoplasmic reticulum (ER) and PtdIns(4,5)P2 is generated in the PM, it has been postulated that PtdIns transfer proteins (PITPs) provide the means for this lipid transfer function. Recent studies identified the large PITP protein, Nir2 as important for PtdIns transfer from the ER to the PM. It was also found that Nir2 was required for the transfer of phosphatidic acid (PtdOH) from the PM to the ER. In Nir2-depleted cells, activation of PLC leads to PtdOH accumulation in the PM and PtdIns synthesis becomes severely impaired. In quiescent cells, Nir2 is localized to the ER via interaction of its FFAT domain with ER-bound VAMP-associated proteins VAP-A and-B. After PLC activation, Nir2 also binds to the PM via interaction of its C-terminal domains with diacylglycerol (DAG) and PtdOH. Through these interactions, Nir2 functions in ER-PM contact zones. Mutations in VAP-B that have been identified in familial forms of amyotrophic lateral sclerosis (ALS or Lou-Gehrig's disease) cause aggregation of the VAP-B protein, which then impairs its binding to several proteins, including Nir2. These findings have shed new lights on the importance of non-vesicular lipid transfer of PtdIns and PtdOH in ER-PM contact zones with a possible link to a devastating human disease.

  18. Grsf1-induced translation of the SNARE protein Use1 is required for expansion of the erythroid compartment.

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    Andrzej Nieradka

    Full Text Available Induction of cell proliferation requires a concomitant increase in the synthesis of glycosylated lipids and membrane proteins, which is dependent on ER-Golgi protein transport by CopII-coated vesicles. In this process, retrograde transport of ER resident proteins from the Golgi is crucial to maintain ER integrity, and allows for anterograde transport to continue. We previously showed that expression of the CopI specific SNARE protein Use1 (Unusual SNARE in the ER 1 is tightly regulated by eIF4E-dependent translation initiation of Use1 mRNA. Here we investigate the mechanism that controls Use1 mRNA translation. The 5'UTR of mouse Use1 contains a 156 nt alternatively spliced intron. The non-spliced form is the predominantly translated mRNA. The alternatively spliced sequence contains G-repeats that bind the RNA-binding protein G-rich sequence binding factor 1 (Grsf1 in RNA band shift assays. The presence of these G-repeats rendered translation of reporter constructs dependent on the Grsf1 concentration. Down regulation of either Grsf1 or Use1 abrogated expansion of erythroblasts. The 5'UTR of human Use1 lacks the splice donor site, but contains an additional upstream open reading frame in close proximity of the translation start site. Similar to mouse Use1, also the human 5'UTR contains G-repeats in front of the start codon. In conclusion, Grsf1 controls translation of the SNARE protein Use1, possibly by positioning the 40S ribosomal subunit and associated translation factors in front of the translation start site.

  19. Primary cilia are not required for normal canonical Wnt signaling in the mouse embryo.

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    Polloneal Jymmiel R Ocbina

    Full Text Available Sonic hedgehog (Shh signaling in the mouse requires the microtubule-based organelle, the primary cilium. The primary cilium is assembled and maintained through the process of intraflagellar transport (IFT and the response to Shh is blocked in mouse mutants that lack proteins required for IFT. Although the phenotypes of mouse IFT mutants do not overlap with phenotypes of known Wnt pathway mutants, recent studies report data suggesting that the primary cilium modulates responses to Wnt signals.We therefore carried out a systematic analysis of canonical Wnt signaling in mutant embryos and cells that lack primary cilia because of loss of the anterograde IFT kinesin-II motor (Kif3a or IFT complex B proteins (Ift172 or Ift88. We also analyzed mutant embryos with abnormal primary cilia due to defects in retrograde IFT (Dync2h1. The mouse IFT mutants express the canonical Wnt target Axin2 and activate a transgenic canonical Wnt reporter, BAT-gal, in the normal spatial pattern and to the same quantitative level as wild type littermates. Similarly, mouse embryonic fibroblasts (MEFs derived from IFT mutants respond normally to added Wnt3a. The switch from canonical to non-canonical Wnt also appears normal in IFT mutant MEFs, as both wild-type and mutant cells do not activate the canonical Wnt reporter in the presence of both Wnt3a and Wnt5a.We conclude that loss of primary cilia or defects in retrograde IFT do not affect the response of the midgestation embryo or embryo-derived fibroblasts to Wnt ligands.

  20. Analysis of connection element classes and locations and of some structural requirements for the mounting of different superstructure types on transport vehicles

    Directory of Open Access Journals (Sweden)

    Zoran Đ. Majkić

    2011-04-01

    Full Text Available The paper presents the basic requirements for transport vehicles. A special request regarding the adaptation of transport vehicles for the transport of various types of cargo was taken into consideration. Superstructures and the situation arising after mounting superstructures on wheeled transport vehicles were analyzed and the following was described: console coupling, stirrups, simplex elastic coupling, two-way elastic and rigid connection elements. Vehicle torsional elasticity is provided by a proper choice of the type of connection between the superstructure and the vehicle chassis. Applying the instructions of vehicle manufacturers for using appropriate connections between the truck superstructure and the vehicle chassis provides positive torsional elasticity of the vehicle. The paper gives the general recommendations of the Volvo, Mercedes and Renault transport vehicle producers for the use of particular connection types of locations as well as structural requirements for the mounting of concrete mixers, tippers and truck tanks on their vehicles. Introduction Achieving a high level of transport effectiveness depends on a number of factors. One of the most important ones is the possibility to increase the payload share in the gross vehicle weight. This share depends on the net vehicle weight, a method of coupling the truck superstructure with the chassis frame as well as on the truck superstructure construction. Realization of this requirement is of significant importance, particularly for large business systems since it results in the reduction of number of necessary vehicles, more economic fleet maintenance and the fleet capacity increase. It is also relatively easy to adapt the vehicle for the transportation of other loads, depending on user's current needs. The adaptation is correctly performed if manufacturer's recommendations are followed during the mounting of the superstructure on the chassis. This paper gives the analysis of the

  1. Phospholipid translocation and miltefosine potency require both L. donovani miltefosine transporter and the new protein LdRos3 in Leishmania parasites.

    Science.gov (United States)

    Pérez-Victoria, F Javier; Sánchez-Cañete, María P; Castanys, Santiago; Gamarro, Francisco

    2006-08-18

    The antitumor drug miltefosine has been recently approved as the first oral drug active against visceral leishmaniasis. We have previously identified the L. donovani miltefosine transporter (LdMT) as a P-type ATPase involved in phospholipid translocation at the plasma membrane of Leishmania parasites. Here we show that this protein is essential but not sufficient for the phospholipid translocation activity and, thus, for the potency of the drug. Based on recent findings in yeast, we have identified the putative beta subunit of LdMT, named LdRos3, as another protein factor required for the translocation activity. LdRos3 belongs to the CDC50/Lem3 family, proposed as likely beta subunits for P4-ATPases. The phenotype of LdRos3-defective parasites was identical to that of the LdMT-/-, including a defect in the uptake of 7-nitrobenz-2-oxa-1,3-diazol-4-yl-amino)-phosphatidylserine, generally considered as not affected in Lem3p-deficient yeast. Both LdMT and LdRos3 normally localized to the plasma membrane but were retained inside the endoplasmic reticulum in the absence of the other protein or when inactivating point mutations were introduced in LdMT. Modulating the expression levels of either protein independently, we show that any one of them could behave as the protein limiting the level of flippase activity. Thus, LdMT and LdRos3 seem to form part of the same translocation machinery that determines flippase activity and miltefosine sensitivity in Leishmania, further supporting the consideration of CDC50/Lem3 proteins as beta subunits required for the normal functioning of P4-ATPases. PMID:16785229

  2. Detection of the Endosomal Sorting Complex Required for Transport in Entamoeba histolytica and Characterization of the EhVps4 Protein

    Directory of Open Access Journals (Sweden)

    Israel López-Reyes

    2010-01-01

    Full Text Available Eukaryotic endocytosis involves multivesicular bodies formation, which is driven by endosomal sorting complexes required for transport (ESCRT. Here, we showed the presence and expression of homologous ESCRT genes in Entamoeba histolytica. We cloned and expressed the Ehvps4 gene, an ESCRT member, to obtain the recombinant EhVps4 and generate specific antibodies, which immunodetected EhVps4 in cytoplasm of trophozoites. Bioinformatics and biochemical studies evidenced that rEhVps4 is an ATPase, whose activity depends on the conserved E211 residue. Next, we generated trophozoites overexpressing EhVps4 and mutant EhVps4-E211Q FLAG-tagged proteins. The EhVps4-FLAG was located in cytosol and at plasma membrane, whereas the EhVps4-E211Q-FLAG was detected as abundant cytoplasmic dots in trophozoites. Erythrophagocytosis, cytopathic activity, and hepatic damage in hamsters were not improved in trophozoites overexpressing EhVps4-FLAG. In contrast, EhVps4-E211Q-FLAG protein overexpression impaired these properties. The localization of EhVps4-FLAG around ingested erythrocytes, together with our previous results, strengthens the role for EhVps4 in E. histolytica phagocytosis and virulence.

  3. Multiple di-leucines in the ATP7A copper transporter are required for retrograde trafficking to the trans-Golgi network.

    Science.gov (United States)

    Zhu, Sha; Shanbhag, Vinit; Hodgkinson, Victoria L; Petris, Michael J

    2016-09-01

    The ATP7A protein is a ubiquitous copper-transporting P-type ATPase that is mutated in the lethal pediatric disorder of copper metabolism, Menkes disease. The steady-state location of ATP7A is within the trans-Golgi network (TGN), where it delivers copper to copper-dependent enzymes within the secretory pathway. However, ATP7A constantly cycles between the TGN and the plasma membrane, and in the presence of high copper concentrations, the exocytic arm of this cycling pathway is enhanced to promote a steady-state distribution of ATP7A to post-Golgi vesicles and the plasma membrane. A single di-leucine endocytic motif within the cytosolic carboxy tail of ATP7A (1487LL) was previously shown to be essential for TGN localization by functioning in retrieval from the plasma membrane, however, the requirement of other di-leucine signals in this region has not been fully investigated. While there has been some success in identifying sequence elements within ATP7A required for trafficking and catalysis, progress has been hampered by the instability of the ATP7A cDNA in high-copy plasmids during replication in Escherichia coli. In this study, we find that the use of DNA synthesis to generate silent mutations across the majority of both mouse and human ATP7A open reading frames was sufficient to stabilize these genes in high-copy plasmids, thus permitting the generation of full-length expression constructs. Using the stabilized mouse Atp7a construct, we identify a second di-leucine motif in the carboxy tail of ATP7A (1459LL) as essential for steady-state localization in the TGN by functioning in endosome-to-TGN trafficking. Taken together, these findings demonstrate that multiple di-leucine signals are required for recycling ATP7A from the plasma membrane to the TGN and illustrate the utility of large-scale codon reassignment as a simple and effective approach to circumvent cDNA instability in high-copy plasmids.

  4. Lipopolysaccharide (LPS) inner-core phosphates are required for complete LPS synthesis and transport to the outer membrane in Pseudomonas aeruginosa PAO1.

    Science.gov (United States)

    Delucia, Angela M; Six, David A; Caughlan, Ruth E; Gee, Patricia; Hunt, Ian; Lam, Joseph S; Dean, Charles R

    2011-01-01

    also caused gross changes in cell morphology and led to the accumulation of an aberrant LPS lacking several core sugars and all core phosphates. The aberrant LPS failed to reach the OM, suggesting that WaaP is essential in P. aeruginosa because it is required to produce the full-length LPS that is recognized by the OM transport/assembly machinery in this organism. Therefore, WaaP may constitute a good target for the development of novel antipseudomonal agents.

  5. Hereditary folate malabsorption: A positively charged amino acid at position 113 of the proton-coupled folate transporter (PCFT/SLC46A1) is required for folic acid binding

    Energy Technology Data Exchange (ETDEWEB)

    Lasry, Inbal; Berman, Bluma [The Fred Wyszkowski Cancer Research Laboratory, Dept. of Biology, Technion-Israel Institute of Technology, Haifa 32000 (Israel); Glaser, Fabian [Bioinformatics Knowledge Unit, The Lorry I. Lokey Interdisciplinary Center for Life Sciences and Engineering, Technion, Haifa 32000 (Israel); Jansen, Gerrit [Department of Rheumatology, VU University Medical Center, Amsterdam (Netherlands); Assaraf, Yehuda G., E-mail: assaraf@tx.technion.ac.il [The Fred Wyszkowski Cancer Research Laboratory, Dept. of Biology, Technion-Israel Institute of Technology, Haifa 32000 (Israel)

    2009-08-28

    The proton-coupled folate transporter (PCFT/SLC46A1) mediates intestinal folate uptake at acidic pH. Some loss of folic acid (FA) transport mutations in PCFT from hereditary folate malabsorption (HFM) patients cluster in R113, thereby suggesting a functional role for this residue. Herein, unlike non-conservative substitutions, an R113H mutant displayed 80-fold increase in the FA transport Km while retaining parental Vmax, hence indicating a major fall in folate substrate affinity. Furthermore, consistent with the preservation of 9% of parental transport activity, R113H transfectants displayed a substantial decrease in the FA growth requirement relative to mock transfectants. Homology modeling based on the crystal structures of the Escherichia coli transporter homologues EmrD and glycerol-3-phosphate transporter revealed that the R113H rotamer properly protrudes into the cytoplasmic face of the minor cleft normally occupied by R113. These findings constitute the first demonstration that a basic amino acid at position 113 is required for folate substrate binding.

  6. Laparoscopic Transhiatal Anterograde Inversion Esophagectomy for Upper Esophagus Cancer:Case Report%腹腔镜下经食管裂孔顺行食管拔脱术治疗上段食管癌1例

    Institute of Scientific and Technical Information of China (English)

    张卫强; 刘克强; 裴迎新; 赵京

    2015-01-01

    本文报道1例腹腔镜下顺行食管内翻拔脱术治疗食管癌。患者女,72岁,吞咽不畅1个月,经胃镜和活检病理诊断为上胸段食管鳞状细胞癌。术前分期T1N0M0。2014年12月31日行腹腔镜下顺行食管内翻拔脱术。腹腔镜下用超声刀游离胃、膈食管裂孔和下胸段食管,左颈部切口顺行拔脱食管,经上腹部切口制做管状胃,将管状胃牵至颈部与食管残端吻合。手术过程顺利,手术时间200 min,术中出血量150 ml。术后恢复顺利,第9天进清流食,无呕吐,无反酸,第12天出院。住院期间未发生吻合口漏、声音嘶哑等并发症。术后病理食管鳞癌,淋巴结无转移。术后3个月复诊,进食可,无呕吐,胃酸反流2~3次/d。%[Summary] On December 31 of 2014, laparoscopic transhiatal anterograde inversion esophagectomy was performed in a 72-year-old female patient for upper thoracic esophageal cancer.The indications and eligibility for this surgery were evaluated.The patient presented with a one-month history of progressive dysphagia.Gastroscopy and pathology revealed upper thoracic esophageal squamous cell carcinoma.Chest CT scanning and endoscopic ultrasonography suggested the clinical stage of T1N0M0.Laparoscopic transhiatal anterograde inversion esophagectomy was given.The lesser and greater curvature and the distal third of esophagus were mobilized. Meanwhile, the cervical esophagus was mobilized and the cervical lymph nodes were dissected.The laparoscopic transhiatal anterograde esophagectomy was performed and linear staplers were used to create a gastric tube.The gastric tube was retracted to the neck.The esophagogastric anastomosis was performed.The operation time was 200 min and the intraoperative blood loss was about 150 ml.The patient was allowed to have liquid diet from the 9th post-operative day.The length of postoperative hospital stay was 12 days.No emptying dysfunction and hoarseness occurred

  7. 29 CFR 500.120 - Insurance policy or liability bond is required for each vehicle used to transport any migrant or...

    Science.gov (United States)

    2010-07-01

    ... vehicle used to transport any migrant or seasonal agricultural worker. 500.120 Section 500.120 Labor Regulations Relating to Labor (Continued) WAGE AND HOUR DIVISION, DEPARTMENT OF LABOR REGULATIONS MIGRANT AND SEASONAL AGRICULTURAL WORKER PROTECTION Motor Vehicle Safety and Insurance for Transportation of...

  8. Vesicular glutamate transporter 2 is required for the respiratory and parasympathetic activation produced by optogenetic stimulation of catecholaminergic neurons in the rostral ventrolateral medulla of mice in vivo.

    Science.gov (United States)

    Abbott, Stephen B G; Holloway, Benjamin B; Viar, Kenneth E; Guyenet, Patrice G

    2014-01-01

    Catecholaminergic neurons of the rostral ventrolateral medulla (RVLM-CA neurons; C1 neurons) contribute to the sympathetic, parasympathetic and neuroendocrine responses elicited by physical stressors such as hypotension, hypoxia, hypoglycemia, and infection. Most RVLM-CA neurons express vesicular glutamate transporter (VGLUT)2, and may use glutamate as a ionotropic transmitter, but the importance of this mode of transmission in vivo is uncertain. To address this question, we genetically deleted VGLUT2 from dopamine-β-hydroxylase-expressing neurons in mice [DβH(Cre/0) ;VGLUT2(flox/flox) mice (cKO mice)]. We compared the in vivo effects of selectively stimulating RVLM-CA neurons in cKO vs. control mice (DβH(Cre/0) ), using channelrhodopsin-2 (ChR2-mCherry) optogenetics. ChR2-mCherry was expressed by similar numbers of rostral ventrolateral medulla (RVLM) neurons in each strain (~400 neurons), with identical selectivity for catecholaminergic neurons (90-99% colocalisation with tyrosine hydroxylase). RVLM-CA neurons had similar morphology and axonal projections in DβH(Cre/0) and cKO mice. Under urethane anesthesia, photostimulation produced a similar pattern of activation of presumptive ChR2-positive RVLM-CA neurons in DβH(Cre/0) and cKO mice. Photostimulation in conscious mice produced frequency-dependent respiratory activation in DβH(Cre/0) mice but no effect in cKO mice. Similarly, photostimulation under urethane anesthesia strongly activated efferent vagal nerve activity in DβH(Cre/0) mice only. Vagal responses were unaffected by α1 -adrenoreceptor blockade. In conclusion, two responses evoked by RVLM-CA neuron stimulation in vivo require the expression of VGLUT2 by these neurons, suggesting that the acute autonomic responses driven by RVLM-CA neurons are mediated by glutamate.

  9. VgrG C terminus confers the type VI effector transport specificity and is required for binding with PAAR and adaptor-effector complex.

    Science.gov (United States)

    Bondage, Devanand D; Lin, Jer-Sheng; Ma, Lay-Sun; Kuo, Chih-Horng; Lai, Erh-Min

    2016-07-01

    Type VI secretion system (T6SS) is a macromolecular machine used by many Gram-negative bacteria to inject effectors/toxins into eukaryotic hosts or prokaryotic competitors for survival and fitness. To date, our knowledge of the molecular determinants and mechanisms underlying the transport of these effectors remains limited. Here, we report that two T6SS encoded valine-glycine repeat protein G (VgrG) paralogs in Agrobacterium tumefaciens C58 specifically control the secretion and interbacterial competition activity of the type VI DNase toxins Tde1 and Tde2. Deletion and domain-swapping analysis identified that the C-terminal extension of VgrG1 specifically confers Tde1 secretion and Tde1-dependent interbacterial competition activity in planta, and the C-terminal variable region of VgrG2 governs this specificity for Tde2. Functional studies of VgrG1 and VgrG2 variants with stepwise deletion of the C terminus revealed that the C-terminal 31 aa (C31) of VgrG1 and 8 aa (C8) of VgrG2 are the molecular determinants specifically required for delivery of each cognate Tde toxin. Further in-depth studies on Tde toxin delivery mechanisms revealed that VgrG1 interacts with the adaptor/chaperone-effector complex (Tap-1-Tde1) in the absence of proline-alanine-alanine-arginine (PAAR) and the VgrG1-PAAR complex forms independent of Tap-1 and Tde1. Importantly, we identified the regions involved in these interactions. Although the entire C31 segment is required for binding with the Tap-1-Tde1 complex, only the first 15 aa of this region are necessary for PAAR binding. These results suggest that the VgrG1 C terminus interacts sequentially or simultaneously with the Tap-1-Tde1 complex and PAAR to govern Tde1 translocation across bacterial membranes and delivery into target cells for antibacterial activity. PMID:27313214

  10. VgrG C terminus confers the type VI effector transport specificity and is required for binding with PAAR and adaptor–effector complex

    Science.gov (United States)

    Bondage, Devanand D.; Lin, Jer-Sheng; Ma, Lay-Sun; Kuo, Chih-Horng; Lai, Erh-Min

    2016-01-01

    Type VI secretion system (T6SS) is a macromolecular machine used by many Gram-negative bacteria to inject effectors/toxins into eukaryotic hosts or prokaryotic competitors for survival and fitness. To date, our knowledge of the molecular determinants and mechanisms underlying the transport of these effectors remains limited. Here, we report that two T6SS encoded valine-glycine repeat protein G (VgrG) paralogs in Agrobacterium tumefaciens C58 specifically control the secretion and interbacterial competition activity of the type VI DNase toxins Tde1 and Tde2. Deletion and domain-swapping analysis identified that the C-terminal extension of VgrG1 specifically confers Tde1 secretion and Tde1-dependent interbacterial competition activity in planta, and the C-terminal variable region of VgrG2 governs this specificity for Tde2. Functional studies of VgrG1 and VgrG2 variants with stepwise deletion of the C terminus revealed that the C-terminal 31 aa (C31) of VgrG1 and 8 aa (C8) of VgrG2 are the molecular determinants specifically required for delivery of each cognate Tde toxin. Further in-depth studies on Tde toxin delivery mechanisms revealed that VgrG1 interacts with the adaptor/chaperone–effector complex (Tap-1–Tde1) in the absence of proline-alanine-alanine-arginine (PAAR) and the VgrG1–PAAR complex forms independent of Tap-1 and Tde1. Importantly, we identified the regions involved in these interactions. Although the entire C31 segment is required for binding with the Tap-1–Tde1 complex, only the first 15 aa of this region are necessary for PAAR binding. These results suggest that the VgrG1 C terminus interacts sequentially or simultaneously with the Tap-1–Tde1 complex and PAAR to govern Tde1 translocation across bacterial membranes and delivery into target cells for antibacterial activity. PMID:27313214

  11. Transport of MOX fuel

    International Nuclear Information System (INIS)

    The regulatory framework which governs the transport of MOX fuel is set out, including packages, transport modes and security requirements. Technical requirements for the packages are reviewed and BNFL's experience in plutonium and MOX fuel transport is described. The safety of such operations and the public perception of safety are described and the question of gaining public acceptance for MOX fuel transport is addressed. The paper concludes by emphasising the need for proactive programmes to improve the public acceptance of these operations. (Author)

  12. The Hydroxyl Side Chain of a Highly Conserved Serine Residue Is Required for Cation Selectivity and Substrate Transport in the Glial Glutamate Transporter GLT-1/SLC1A2.

    Science.gov (United States)

    Simonin, Alexandre; Montalbetti, Nicolas; Gyimesi, Gergely; Pujol-Giménez, Jonai; Hediger, Matthias A

    2015-12-18

    Glutamate transporters maintain synaptic concentration of the excitatory neurotransmitter below neurotoxic levels. Their transport cycle consists of cotransport of glutamate with three sodium ions and one proton, followed by countertransport of potassium. Structural studies proposed that a highly conserved serine located in the binding pocket of the homologous GltPh coordinates L-aspartate as well as the sodium ion Na1. To experimentally validate these findings, we generated and characterized several mutants of the corresponding serine residue, Ser-364, of human glutamate transporter SLC1A2 (solute carrier family 1 member 2), also known as glutamate transporter GLT-1 and excitatory amino acid transporter EAAT2. S364T, S364A, S364C, S364N, and S364D were expressed in HEK cells and Xenopus laevis oocytes to measure radioactive substrate transport and transport currents, respectively. All mutants exhibited similar plasma membrane expression when compared with WT SLC1A2, but substitutions of serine by aspartate or asparagine completely abolished substrate transport. On the other hand, the threonine mutant, which is a more conservative mutation, exhibited similar substrate selectivity, substrate and sodium affinities as WT but a lower selectivity for Na(+) over Li(+). S364A and S364C exhibited drastically reduced affinities for each substrate and enhanced selectivity for L-aspartate over D-aspartate and L-glutamate, and lost their selectivity for Na(+) over Li(+). Furthermore, we extended the analysis of our experimental observations using molecular dynamics simulations. Altogether, our findings confirm a pivotal role of the serine 364, and more precisely its hydroxyl group, in coupling sodium and substrate fluxes.

  13. Transportation of radioactive and hazardous materials: a summary of state and local legislative requirements for the period ending September 30, 1983

    International Nuclear Information System (INIS)

    The Legislative Data Base (LDB) was developed for Sandia National Laboratories' Transportation Technology Center by Oak Ridge National Laboratory to monitor federal, state, and local legislative and regulatory actions affecting the transport of radioactive materials. The data base contains 2500 citations of introduced, adopted/enacted, and denied legislative actions. This report summarizes in tabular format pertinent enacted or adopted state and local restrictions contained in the LDB

  14. Transport of radioactive materials

    International Nuclear Information System (INIS)

    The purpose of this Norm is to establish, relating to the TRANSPORT OF RADIOACTIVE MATERIALS, safety and radiological protection requirements to ensure an adequate control level of the eventual exposure of persons, properties and environment to the ionizing radiation comprising: specifications on radioactive materials for transport; package type selection; specification of the package design and acceptance test requirements; arrangements relating to the transport itself; administrative requirements and responsibilities. (author)

  15. ydjN encodes an S-sulfocysteine transporter required by Escherichia coli for growth on S-sulfocysteine as a sulfur source.

    Science.gov (United States)

    Yamazaki, Shunsuke; Takei, Kensuke; Nonaka, Gen

    2016-09-01

    Sulfur is an essential element for growth and many physiological functions. As sulfur sources for Escherichia coli and related bacteria, specific transporters import various sulfur-containing compounds from the environment. In this study, we identified and characterized an alternative function of the cystine transporter YdjN in E. coli as a transporter of S-sulfocysteine, a sulfur-containing intermediate in the assimilatory cysteine biosynthesis that is used as a sulfur source for the growth of E. coli We also demonstrated that the transport of S-sulfocysteine via YdjN depends on the transcriptional regulator CysB, a master regulator that controls most of the genes involved in sulfur assimilation and cysteine metabolism. We found that the use of S-sulfocysteine as a sulfur source depends on glutathione because mutations in glutathione biosynthetic genes abolish growth when S-sulfocysteine is used as a sole sulfur source, thereby supporting the previous findings that the conversion of S-sulfocysteine to cysteine is catalyzed by glutaredoxins. To the best of our knowledge, this is the first report of a functional S-sulfocysteine transporter across organisms, which strongly supports the hypothesis that S-sulfocysteine is not only a metabolic intermediate but also a physiologically significant substance in specific natural environments.

  16. The ileal lipid binding protein is required for efficient absorption and transport of bile acids in the distal portion of the murine small intestine.

    Science.gov (United States)

    Praslickova, Dana; Torchia, Enrique C; Sugiyama, Michael G; Magrane, Elijah J; Zwicker, Brittnee L; Kolodzieyski, Lev; Agellon, Luis B

    2012-01-01

    The ileal lipid binding protein (ilbp) is a cytoplasmic protein that binds bile acids with high affinity. However evidence demonstrating the role of this protein in bile acid transport and homeostasis is missing. We created a mouse strain lacking ilbp (Fabp6(-/-) mice) and assessed the impact of ilbp deficiency on bile acid homeostasis and transport in vivo. Elimination of ilbp increased fecal bile acid excretion (54.2%, Pexcreted by 24 h after oral administration was 102% (Psmall and large intestines was increased by 22% (P<0.02) and decreased by 62.7% (P<0.01), respectively, in male Fabp6(-/-) mice relative wild-type mice, whereas no changes were seen in female Fabp6(-/-) mice. Mucosal to serosal bile acid transport using everted distal gut sacs was decreased by 74% (P<0.03) in both sexes of Fabp6(-/-) mice as compared to wild-type mice. The results demonstrate that ilbp is involved in the apical to basolateral transport of bile acids in ileal enterocytes, and is vital for the maintenance of bile acid homeostasis in the enterohepatic circulation (EHC) in mice.

  17. Transmembrane segment (TMS) VIII of the Na+/citrate transporter CitS requires downstream TMS IX for insertion in the Escherichia coli membrane

    NARCIS (Netherlands)

    van Geest, M; Lolkema, JS

    1999-01-01

    The amino acid sequence of the sodium ion-dependent citrate transporter CitS of IL pneumoniae contains 12 hydrophobic stretches that could form membrane-spanning segments. A previous analysis of the membrane topology in Escherichia coli using the PhoA gene fusion technique indicated that only nine o

  18. Requirement for the gravity-controlled transport of auxin for a negative gravitropic response of epicotyls in the early growth stage of etiolated pea seedlings.

    Science.gov (United States)

    Hoshino, Tomoki; Miyamoto, Kensuke; Ueda, Junichi

    2006-11-01

    Gravity-controlled transport of auxin was studied for a negative gravitropic response in the early growth stage of etiolated pea (Pisum sativum L. cv. Alaska) seedlings, in which epicotyl bending was observed near the cotyledon nodes of the seedlings grown continuously from seeds germinated in a horizontal or an inclined position. Increased expression of an auxin-inducible gene, PsIAA4/5, was observed in the elongated side of epicotyls grown in a horizontal or an inclined position. Regardless of the conditions of seed germination, polar auxin transport in the proximal side of the first internodes of the seedlings was significantly higher than in the distal side. Polar auxin transport in the proximal side of epicotyls grown in an inclined position was significantly lower than in those grown in a horizontal position. In contrast, lateral auxin distribution from the proximal to distal sides in epicotyls grown in an inclined position was significantly higher than in epicotyls grown in a horizontal position. Accumulation of PsPIN1 mRNA encoding a putative auxin efflux facilitator, which was observed in vascular tissue, cortex and epidermis in the proximal and distal sides of epicotyls, was markedly influenced by gravistimulation. These results strongly suggest that gravistimulation induces changeable polar auxin transport and one-way lateral auxin distribution in epicotyls as well as asymmetric auxin accumulation in the proximal and distal sides of epicotyls, resulting in a negative gravitropic response of epicotyls in the early growth stage of pea seedlings. PMID:17008444

  19. Fleet servicing facilities for servicing, maintaining, and testing rail and truck radioactive waste transport systems: functional requirements, technical design concepts and options cost estimates and comparisons

    International Nuclear Information System (INIS)

    This is a resource document which examines feasibility design concepts and feasibility studies of a Fleet Servicing Facility (FSF). Such a facility is intended to be used for routine servicing, preventive maintenance, and for performing requalification license compliance tests and inspections, minor repairs, and decontamination of both the transportation casks and their associated rail cars or tractor-trailers. None of the United States' waste handling plants presently receiving radioactive wastes have an on-site FSF, nor is there an existing third party facility providing these services. This situation has caused the General Accounting Office to express concern regarding the quality of waste transport system maintenance once the system is placed into service. Thus, a need is indicated for FSF's, or their equivalent, at various radioactive materials receiving sites. In this report, three forms of FSF's solely for spent fuel transport systems were examined: independent, integrated, and colocated. The independent concept was already the subject of a detailed report and is extensively referenced in this document so that capital cost comparisons of the three concepts could be made. These facilities probably could service high-level, intermediate-level, low-level, or other waste transportation systems with minor modification, but this study did not include any system other than spent fuel. Both the Integrated and Colocated concepts were assumed to be associated with some radioactive materials handling facility such as an AFR repository

  20. The predicted ABC transporter AbcEDCBA is required for type IV secretion system expression and lysosomal evasion by Brucella ovis.

    Directory of Open Access Journals (Sweden)

    Teane M A Silva

    Full Text Available Brucella ovis is a major cause of reproductive failure in rams and it is one of the few well-described Brucella species that is not zoonotic. Previous work showed that a B. ovis mutant lacking a species-specific ABC transporter (ΔabcBA was attenuated in mice and was unable to survive in macrophages. The aim of this study was to evaluate the role of this ABC transporter during intracellular survival of B. ovis. In HeLa cells, B. ovis WT was able to survive and replicate at later time point (48 hpi, whereas an ΔabcBA mutant was attenuated at 24 hpi. The reduced survival of the ΔabcBA mutant was associated with a decreased ability to exclude the lysosomal marker LAMP1 from its vacuolar membrane, suggesting a failure to establish a replicative niche. The ΔabcBA mutant showed a reduced abundance of the Type IV secretion system (T4SS proteins VirB8 and VirB11 in both rich and acid media, when compared to WT B. ovis. However, mRNA levels of virB1, virB8, hutC, and vjbR were similar in both strains. These results support the notion that the ABC transporter encoded by abcEDCBA or its transported substrate acts at a post-transcriptional level to promote the optimal expression of the B. ovis T4SS within infected host cells.

  1. The N-terminal extension domain of the C. elegans half-molecule ABC transporter, HMT-1, is required for protein-protein interactions and function.

    Directory of Open Access Journals (Sweden)

    Sungjin Kim

    Full Text Available BACKGROUND: Members of the HMT-1 (heavy metal tolerance factor 1 subfamily of the ATP-binding cassette (ABC transporter superfamily detoxify heavy metals and have unique topology: they are half-molecule ABC transporters that, in addition to a single transmembrane domain (TMD1 and a single nucleotide-binding domain (NBD1, possess a hydrophobic NH2-terminal extension (NTE. These structural features distinguish HMTs from other ABC transporters in different species including Drosophila and humans. Functional ABC transporters, however, are comprised of at least four-domains (two TMDs and two NDBs formed from either a single polypeptide or by the association of two or four separate subunits. Whether HMTs act as oligomers and what role the NTE domain plays in their function have not been determined. METHODOLOGY/PRINCIPAL FINDINGS: In this study, we examined the oligomeric status of Caenorhabditis elegans HMT-1 and the functional significance of its NTE using gel-filtration chromatography in combination with the mating-based split-ubiquitin yeast two-hybrid system (mbSUS and functional in vivo assays. We found that HMT-1 exists in a protein complex in C. elegans. Studies in S. cerevisiae showed that HMT-1 at a minimum homodimerizes and that oligomerization is essential for HMT-1 to confer cadmium tolerance. We also established that the NTE domain plays an important structural and functional role: it is essential for HMT-1 oligomerization and Cd-detoxification function. However, the NTE itself was not sufficient for oligomerization suggesting that multiple structural features of HMT-1 must associate to form a functional transporter. CONCLUSIONS: The prominence of heavy metals as environmental toxins and the remarkable conservation of HMT-1 structural architecture and function in different species reinforce the value of continued studies of HMT-1 in model systems for identifying functional domains in HMT1 of humans.

  2. Proposed design requirements for high-integrity containers used to store, transport, and dispose of high-specific-activity, low-level radioactive wastes from Three Mile Island Unit II

    International Nuclear Information System (INIS)

    This report develops proposed design requirements for high integrity containers used to store, transport and/or dispose of high-activity, low-level radioactive wastes from Three Mile Island Unit II. The wastes considered are the dewatered resins produced by the EPICOR II waste treatment system used to clean-up the auxiliary building water. The radioactivity level of some of these EPICOR II liners is 1300 curies per container. These wastes may be disposed of in an intermediate depth burial (10 to 20 meter depth) facility. The proposed container design requirements are directed to ensure isolation of the waste and protection of the public health and safety

  3. ANTEROGRADE AND RETROGRADE TRANSPORT OF THE PRECURSOR FOR BRAIN-DERIVED NEUROTROPHIC FACTOR IN PRIMARY SENSORY NEURONS%pro-BDNF在初级感觉神经元内的顺行和逆行输送

    Institute of Scientific and Technical Information of China (English)

    王慧; 罗学港; 鞠躬

    2005-01-01

    研究表明脑源性神经营养因子(BDNF)在神经元内可顺行运输至末梢并释放到下一级神经元参与突触可塑性等功能.而成熟的BDNF由其前体分子(pro-BDNF)经蛋白酶水解形成.体外研究表明pro-BDNF可以激活神经营养因子受体TrkB并导致TrkB的磷酸化,但pro-BDNF在体内的作用目前仍不清楚.本文拟探讨内源性pro-BDNF在周围神经的运输.将大鼠坐骨神经结扎或压榨背根制成模型,动物存活不同时间后取坐骨神经、背根神经节和脊髓进行pro-BDNF免疫组织化学染色检测.结果显示:pro-BDNF免疫阳性产物沉积于坐骨神经和背根损伤处的近侧端和远侧端,24 h达高峰,近侧端可持续达7 d而远侧端可达3 d;在坐骨神经的近侧端和远侧端、背根神经节和脊髓可检测到全分子量大小的完整pro-BDNF;在转染pro-BDNF质粒后,PC12细胞内可见pro-BDNF免疫阳性产物分布于胞体和突起.这些结果提示pro-BDNF可以象成熟BDNF一样在感觉神经内顺行和逆行运输,并可由神经元分泌、释放而发挥生理作用.

  4. Formulation of detailed consumables management models for the development (preoperational) period of advanced space transportation system. Volume 5: Flight operations processor requirements

    Science.gov (United States)

    Zamora, M. A.

    1976-01-01

    The functional requirements for the Flight Operations Processor are defined. The Flight Operations Processor is that element of the Consumables Management System providing support during the flight operations.

  5. Repairing of fingertip defect with topographical anterograde flap pedicled with digital artery trunk or branch%带指掌侧固有动脉或其分支局部顺行皮瓣修复指端缺损

    Institute of Scientific and Technical Information of China (English)

    林宏伟; 吴杰; 江标; 连素文; 邹育才; 肖瑛; 赵资坚; 林丽贤

    2015-01-01

    目的:探讨带指掌侧固有动脉或其分支局部顺行皮瓣修复指端缺损的临床效果。方法总结2011年6月至2014年6月期间,采用带指掌侧固有动脉或其分支局部顺行皮瓣转移修复的83例118个指端缺损的临床资料。结果92个皮瓣顺利成活。7个背侧旋转皮瓣、5个V-Y推进皮瓣在术后24 h内出现动脉危象,9个背侧旋转皮瓣、5个掌侧旋转皮瓣在术后24 h内出现静脉危象。视循环危象具体情况分别采用拆除皮瓣周边、蒂部部分缝线,皮瓣小切口放血,皮瓣按摩,改变手指体位,患指制动等方法处理。动脉危象皮瓣4个存活,8个部分坏死。静脉危象皮瓣8个存活,6个部分坏死。皮瓣供区植皮57/62例(91.9%)全部成活。67例99指获得3~12个月、平均5.5个月的随访。皮瓣色泽红润、质地柔软、外观自然、不臃肿,与周围皮肤接近。皮瓣蒂部不臃肿。指端饱满,外形良好。两点辨别觉8~12 mm,无痛性瘢痕形成,无严重触痛。患指各关节活动基本正常,无关节坚硬。患者能适应正常工作与生活,对指端感觉及伤指外形均较满意。按中华医学会手外科学会上肢部分功能评定试用标准评定,优63指,良20指,可16指,优良率83.8%。结论带指掌侧固有动脉或其分支局部顺行皮瓣修复指端缺损,方法简便,无需复杂显微外科技术,效果满意,对供区影响小,成功率高,值得临床推广应用。%Objective To investigate the clinical outcomes of repairing fingertip defects by transferring topographical anterograde flaps pedicled with digital artery trunk or branch. Methods From Jun. 2011 to Jun. 2014, 118 fingers in 83 cases with fingertip defects were treated with topographical anterograde flaps pedicled with digital artery trunk or branch. We recorded and generalized the clinical materials. Results 92 flaps survived uneventfully. 7 digital artery dorsal

  6. 咪唑安定和丙泊酚联用对危重患者镇静-遗忘作用的研究%Effects of combination of midazolam and propofol on anterograde amnesia in critical patients

    Institute of Scientific and Technical Information of China (English)

    许继元; 戴体俊; 李茂琴; 张舟; 卢飞; 李琳; 李家琼; 莫逊; 许艳军; 刘君

    2008-01-01

    目的 观察咪唑安定和丙泊酚联用对危重患者镇静-遗忘作用的影响.方法 选择重症加强治疗病房(ICU)行机械通气2~4 d的患者60例,随机分为丙泊酚组、咪唑安定组和咪唑安定加丙泊酚联用组,每组20例.分别于用药后1、2和3 d唤醒患者并出示不同颜色、图形、数字的卡片,于患者停用机械通气完全清醒后评估药物对其镇静-遗忘作用的影响.结果 ①丙泊酚、咪唑安定和联用组分别有70%、95%和90%的患者产生遗忘,停药30 min后患者均恢复记忆.②咪唑安定组静脉推注负荷量药物起效的时间((5.1±2.8)min]和停药清醒后拔除气管插管的时间[(2.7±0.3)h]均较丙泊酚组[(2.7±1.1)min、(0.7±0.2)h]、联用组[(3.1±1.3)min、(1.2±0.6)h]明显延长(P均<0.01);丙泊酚组和联用组药物起效时间和停药清醒后拔除气管插管时间相近,差异无统计学意义(P均>0.05).③镇静费用咪唑安定组[(1 200±112)元]和联用组[(1 300±132)元]接近,丙泊酚组[(2 100±125)元]高于咪唑安定组约75%(P<0.01).结论 丙泊酚与咪唑安定联合用药既可确保患者产生镇静-遗忘效应,减少各自的用量,降低药物不良反应,又有利于降低患者的住院费用,可能是ICU危重患者较好的镇静-遗忘治疗方案.%Objective To observe the effects of sedation with midazolam and propofol on anterograde amnesia in critical patients.Methods Sixty selected patients on mechanical ventilation in intensive care unit (ICU)were randomly divided into three subgroups(propofol,midazolam,and midazolam and propofol combination group),with 20 cases in each group.Patients who were awakened from sedation were showed with a card depicted with different colors,figures and numbers.When patients were totally conscious after weaning from mechanicaI ventilation,the influence of the different methods of sedation on anterograde amnesia in propofol,midazolam and the combination group

  7. Secure Transportation Management

    Energy Technology Data Exchange (ETDEWEB)

    Gibbs, P. W. [Brookhaven National Lab. (BNL), Upton, NY (United States)

    2014-10-15

    Secure Transport Management Course (STMC) course provides managers with information related to procedures and equipment used to successfully transport special nuclear material. This workshop outlines these procedures and reinforces the information presented with the aid of numerous practical examples. The course focuses on understanding the regulatory framework for secure transportation of special nuclear materials, identifying the insider and outsider threat(s) to secure transportation, organization of a secure transportation unit, management and supervision of secure transportation units, equipment and facilities required, training and qualification needed.

  8. Recognition of the Agrobacterium tumefaciens VirE2 translocation signal by the VirB/D4 transport system does not require VirE1.

    Science.gov (United States)

    Vergunst, Annette C; van Lier, Miranda C M; den Dulk-Ras, Amke; Hooykaas, Paul J J

    2003-11-01

    Agrobacterium tumefaciens uses a type IV secretion system to deliver a nucleoprotein complex and effector proteins directly into plant cells. The single-stranded DNA-binding protein VirE2, the F-box protein VirF and VirE3 are delivered into host cells via this VirB/D4 encoded translocation system. VirE1 functions as a chaperone of VirE2 by regulating its efficient translation and preventing VirE2-VirE2 aggregation in the bacterial cell. We analyzed whether the VirE1 chaperone is also essential for transport recognition of VirE2 by the VirB/D4 encoded type IV secretion system. In addition, we assayed whether translocation of VirF and VirE3, which also forms part of the virE operon, is affected by the absence of VirE1. We employed the earlier developed CRAFT (Cre recombinase Reporter Assay For Translocation) assay to detect transfer of Cre::Vir fusion proteins from A. tumefaciens into plants, monitored by stable reconstitution of a kanamycin resistance marker, and into yeast, screened by loss of the URA3 gene. We show that the C-terminal 50 amino acids of VirE2 and VirE3 are sufficient to mediate Cre translocation into host cells, confirming earlier indications of a C-terminal transport signal. This transfer was independent of the presence or absence of VirE1. Besides, the translocation efficiency of VirF is not altered in a virE1 mutant. The results unambiguously show that the VirE1 chaperone is not essential for the recognition of the VirE2 transport signal by the transport system and the subsequent translocation across the bacterial envelope into host cells. PMID:14551327

  9. The ileal lipid binding protein is required for efficient absorption and transport of bile acids in the distal portion of the murine small intestine.

    Directory of Open Access Journals (Sweden)

    Dana Praslickova

    Full Text Available The ileal lipid binding protein (ilbp is a cytoplasmic protein that binds bile acids with high affinity. However evidence demonstrating the role of this protein in bile acid transport and homeostasis is missing. We created a mouse strain lacking ilbp (Fabp6(-/- mice and assessed the impact of ilbp deficiency on bile acid homeostasis and transport in vivo. Elimination of ilbp increased fecal bile acid excretion (54.2%, P<0.05 in female but not male Fabp6(-/- mice. The activity of cholesterol 7α-hydroxylase (cyp7a1, the rate-controlling enzyme of the classical bile acid biosynthetic pathway, was significantly increased in female (63.5%, P<0.05 but not in male Fabp6(-/- mice. The amount of [(3H]taurocholic acid (TCA excreted by 24 h after oral administration was 102% (P<0.025 higher for female Fabp6(-/- mice whereas it was 57.3% (P<0.01 lower for male Fabp6(-/- mice, compared to wild-type mice. The retained fraction of the [(3H]TCA localized in the small and large intestines was increased by 22% (P<0.02 and decreased by 62.7% (P<0.01, respectively, in male Fabp6(-/- mice relative wild-type mice, whereas no changes were seen in female Fabp6(-/- mice. Mucosal to serosal bile acid transport using everted distal gut sacs was decreased by 74% (P<0.03 in both sexes of Fabp6(-/- mice as compared to wild-type mice. The results demonstrate that ilbp is involved in the apical to basolateral transport of bile acids in ileal enterocytes, and is vital for the maintenance of bile acid homeostasis in the enterohepatic circulation (EHC in mice.

  10. Fast Charging Battery Buses for the Electrification of Urban Public Transport : A Feasibility Study Focusing on Charging Infrastructure and Energy Storage Requirements

    OpenAIRE

    Matthias Rogge; Sebastian Wollny; Dirk Uwe Sauer

    2015-01-01

    The electrification of public transport bus networks can be carried out utilizing different technological solutions, like trolley, battery or fuel cell buses. The purpose of this paper is to analyze how and to what extent existing bus networks can be electrified with fast charging battery buses. The so called opportunity chargers use mainly the regular dwell time at the stops to charge their batteries. This results in a strong linkage between the vehicle scheduling and the infrastructure plan...

  11. The maize glossy13 gene, cloned via BSR-Seq and Seq-walking encodes a putative ABC transporter required for the normal accumulation of epicuticular waxes.

    Directory of Open Access Journals (Sweden)

    Li Li

    Full Text Available Aerial plant surfaces are covered by epicuticular waxes that among other purposes serve to control water loss. Maize glossy mutants originally identified by their "glossy" phenotypes exhibit alterations in the accumulation of epicuticular waxes. By combining data from a BSR-Seq experiment and the newly developed Seq-Walking technology, GRMZM2G118243 was identified as a strong candidate for being the glossy13 gene. The finding that multiple EMS-induced alleles contain premature stop codons in GRMZM2G118243, and the one knockout allele of gl13, validates the hypothesis that gene GRMZM2G118243 is gl13. Consistent with this, GRMZM2G118243 is an ortholog of AtABCG32 (Arabidopsis thaliana, HvABCG31 (barley and OsABCG31 (rice, which encode ABCG subfamily transporters involved in the trans-membrane transport of various secondary metabolites. We therefore hypothesize that gl13 is involved in the transport of epicuticular waxes onto the surfaces of seedling leaves.

  12. The investigation of different doses of dexmedetomidine on the sedation and anterograde amnesia%不同剂量右美托咪定镇静程度及顺行性遗忘作用研究

    Institute of Scientific and Technical Information of China (English)

    胡志超; 孔莉; 许鹏程; 李颖; 董晓辉

    2015-01-01

    Objective To investigate effects of different doses of dexmedetomidine (Dex) on the sedation and anterograde amnesia in patients undergoing operation on bythus.Methods Two hundred patients,ASA Ⅰ-Ⅱ,aged 20-60,101 males and 99 females were dministered spinal-epidural anesthesia combined with Dex,undergoing operation on bythus without caesarean section.After Dex 1 μg/kg was infused intravenously 10 min (group De0 was infused intravenously the same volume of physiological saline),according to different maintenance doses of Dex,they were divided into four groups by random number table method:group De0 (group physiological saline),group De1 (0.2 μg·kg1·h-1),group De2 (0.4 μg·kg-1·h-1),group De3 (0.6 μg·kg-1·h-1).By observer's assessment of alertness/sedation(OAA/S) method,the sedation degree of the Dex was evaluated at 5 min(T1),10 min(T2),15 min (T3),20 min(T4),25 min(T5),30 min(T6),40 min(T7),50 min(T8).After 24 h of operation,the anterograde amnesia degree of Dex was assessed.Results When the OAMS scores of group De0 of sedation were all(5.00±0) at T2,T3,T4,T5,T6,T7,and T8 point,with the time of Dex infused intravenously longer and the dose of Dex increased,the OAA/S scores of group De1,group De2 and group De3[The OAMS scores of group De1 of sedation was(3.15±0.37) at T2 point,(3.26±0.44) at T3 point,(2.70±0.66) at T4 point,(2.55±0.60) at T5 point,(2.40±0.60) at T2 point,(2.05±0.76) at T7 point,(2.02±0.73) at T8 point,The OAA/S scores of group De2 of sedation was (3.10±0.64) at T2 point,(2.95±0.51) at T3 point,(2.35±0.67) at T4 point,(2.25±0.55) at T5 point,(2.10±0.45) at T6 point,(1.60±0.50) at T7 point,(1.65±0.49) at T8 point;The OAMS scores of group De3 of sedation was (3.10±0.31) at T2 point,(2.65±0.49) at T3 point,(1.95±0.39) at T4 point,(1.90±0.45) at T5 point,(1.75±0.44) at T6 point,(1.20±0.62) at T7 point,(1.25± 0.64) at T8 point] became smaller in a dose-dependent manner(P<0.05).When there were 50 cases of the no

  13. REQUIREMENTS FOR STORAGE AND TRANSPORT OF BIOTECHNOLOGICAL MEDICAL PRODUCTS IN ACCORDANCE WITH THE REGULATORY FRAMEWORK OF MINISTRY OF PUBLIC HEALTH OF UKRAINE AND DETECTION OF VIOLATIONS IN THE MEDICAL AND PHARMACEUTICAL INSTITUTIONS

    Directory of Open Access Journals (Sweden)

    Shukaeva O.

    2015-05-01

    Full Text Available Introduction. The rapid development of the pharmaceutical industry and the expansion of the range of biotech drugs require special conditions to ensure the quality, storage and transport through out the entire chain: manufacturer - distributor - pharmacy - hospital - the patient.We analyzed the current legislative frame work of Ministry of Public Health of Ukraine and conducted a study to identify and analyze of typical violations in the medical and pharmaceutical institutions. The aim of the investigation was to investigate and analyze inspection acts under storage and transport of biological medical products and identify major violations during the performance requirements for storage and transportation of drugs, level of awareness about medical products which are requiring special storage requirement. Methods: systemic, logistical, structural, marketing, regulatory. Results & discussion. According to the data presented in the report «Assessing biosimilar uptake and competition in European markets» of «IMS Health», sales of medical products with biological nature - biological medicinal products and biosymilyars is about 27% of total sales of drugs in the EU. This segment of the pharmaceutical market is characterized by faster growth compared to the pharmaceutical marketas a whole. Thus, in 2012-2013 years sales of biological medical products in the EU countries increased by 5.5% compared to 1.5% increase in total sales of drugs. It is important that in Europe, according to the 2013 preparations, the market share in value terms, with eight to prepare biological products, the term of patent protection that are either already expired or will expireby 2020, and therefore they can be competitors with biosymilars. In creasing the number of medications on the market requires a careful approach of storing and preserving the quality of distribution during throughout the life of the medical products in the chain: manufacturer - distributor

  14. Comparative genomic analysis of slc39a12/ZIP12: insight into a zinc transporter required for vertebrate nervous system development.

    Directory of Open Access Journals (Sweden)

    Winyoo Chowanadisai

    Full Text Available The zinc transporter ZIP12, which is encoded by the gene slc39a12, has previously been shown to be important for neuronal differentiation in mouse Neuro-2a neuroblastoma cells and primary mouse neurons and necessary for neurulation during Xenopus tropicalis embryogenesis. However, relatively little is known about the biochemical properties, cellular regulation, or the physiological role of this gene. The hypothesis that ZIP12 is a zinc transporter important for nervous system function and development guided a comparative genetics approach to uncover the presence of ZIP12 in various genomes and identify conserved sequences and expression patterns associated with ZIP12. Ortholog detection of slc39a12 was conducted with reciprocal BLAST hits with the amino acid sequence of human ZIP12 in comparison to the human paralog ZIP4 and conserved local synteny between genomes. ZIP12 is present in the genomes of almost all vertebrates examined, from humans and other mammals to most teleost fish. However, ZIP12 appears to be absent from the zebrafish genome. The discrimination of ZIP12 compared to ZIP4 was unsuccessful or inconclusive in other invertebrate chordates and deuterostomes. Splice variation, due to the inclusion or exclusion of a conserved exon, is present in humans, rats, and cows and likely has biological significance. ZIP12 also possesses many putative di-leucine and tyrosine motifs often associated with intracellular trafficking, which may control cellular zinc uptake activity through the localization of ZIP12 within the cell. These findings highlight multiple aspects of ZIP12 at the biochemical, cellular, and physiological levels with likely biological significance. ZIP12 appears to have conserved function as a zinc uptake transporter in vertebrate nervous system development. Consequently, the role of ZIP12 may be an important link to reported congenital malformations in numerous animal models and humans that are caused by zinc deficiency.

  15. Comparative genomic analysis of slc39a12/ZIP12: insight into a zinc transporter required for vertebrate nervous system development.

    Science.gov (United States)

    Chowanadisai, Winyoo

    2014-01-01

    The zinc transporter ZIP12, which is encoded by the gene slc39a12, has previously been shown to be important for neuronal differentiation in mouse Neuro-2a neuroblastoma cells and primary mouse neurons and necessary for neurulation during Xenopus tropicalis embryogenesis. However, relatively little is known about the biochemical properties, cellular regulation, or the physiological role of this gene. The hypothesis that ZIP12 is a zinc transporter important for nervous system function and development guided a comparative genetics approach to uncover the presence of ZIP12 in various genomes and identify conserved sequences and expression patterns associated with ZIP12. Ortholog detection of slc39a12 was conducted with reciprocal BLAST hits with the amino acid sequence of human ZIP12 in comparison to the human paralog ZIP4 and conserved local synteny between genomes. ZIP12 is present in the genomes of almost all vertebrates examined, from humans and other mammals to most teleost fish. However, ZIP12 appears to be absent from the zebrafish genome. The discrimination of ZIP12 compared to ZIP4 was unsuccessful or inconclusive in other invertebrate chordates and deuterostomes. Splice variation, due to the inclusion or exclusion of a conserved exon, is present in humans, rats, and cows and likely has biological significance. ZIP12 also possesses many putative di-leucine and tyrosine motifs often associated with intracellular trafficking, which may control cellular zinc uptake activity through the localization of ZIP12 within the cell. These findings highlight multiple aspects of ZIP12 at the biochemical, cellular, and physiological levels with likely biological significance. ZIP12 appears to have conserved function as a zinc uptake transporter in vertebrate nervous system development. Consequently, the role of ZIP12 may be an important link to reported congenital malformations in numerous animal models and humans that are caused by zinc deficiency.

  16. The zinc transporter SLC39A13/ZIP13 is required for connective tissue development; its involvement in BMP/TGF-beta signaling pathways.

    Directory of Open Access Journals (Sweden)

    Toshiyuki Fukada

    Full Text Available BACKGROUND: Zinc (Zn is an essential trace element and it is abundant in connective tissues, however biological roles of Zn and its transporters in those tissues and cells remain unknown. METHODOLOGY/PRINCIPAL FINDINGS: Here we report that mice deficient in Zn transporter Slc39a13/Zip13 show changes in bone, teeth and connective tissue reminiscent of the clinical spectrum of human Ehlers-Danlos syndrome (EDS. The Slc39a13 knockout (Slc39a13-KO mice show defects in the maturation of osteoblasts, chondrocytes, odontoblasts, and fibroblasts. In the corresponding tissues and cells, impairment in bone morphogenic protein (BMP and TGF-beta signaling were observed. Homozygosity for a SLC39A13 loss of function mutation was detected in sibs affected by a unique variant of EDS that recapitulates the phenotype observed in Slc39a13-KO mice. CONCLUSIONS/SIGNIFICANCE: Hence, our results reveal a crucial role of SLC39A13/ZIP13 in connective tissue development at least in part due to its involvement in the BMP/TGF-beta signaling pathways. The Slc39a13-KO mouse represents a novel animal model linking zinc metabolism, BMP/TGF-beta signaling and connective tissue dysfunction.

  17. Justified requirements in private transportation and a recommendation for improving the efficiency of household energy utilisation through the use of small ecologically-friendly or 'ultralight' vehicles for mass private transportation in the 21st century

    International Nuclear Information System (INIS)

    Needs and ownership are sociobiologically manifested in the alter-ego of a Homo sapiens where the natural progression of events (a household being the fundamental microlevel) and the social order, i.e. globalisation, are based on ownership and needs as sacred rights, and for this reason universal values like energy conservation end up as the waste of the mindless worship of consumption. Justified needs are phenomena of a consumerist (egocentric, pragmatic, voluntary) social conscience and instinctive behaviour - an unpredictable cause resulting from freedom being the foundation of the quality of life, socio-economic and political changes but are mutually exclusive to understanding (expressing and gaining deeper and richer knowledge). Inbuilt limits and/or control of consumption, which are already used in household appliances with aforeset processes (goals) for unknown consumers, to achieve large energy savings in 'routine' functions are more effective than attempts to prevent mistakes (lack of user knowledge through repression). A private vehicle, as a symbol of the freedom and quality of life, is a mechanism for achieving 'justified' needs and presents another means of household energy utilisation. The consumer's desires regarding private transportation are not sufficiently reconciled with intelligent microprocessors (expert systems), which achieve (the most) optimal behaviour in the process of transportation. This detailed consideration (as part of investigating the technical system) cannot be examined on a strictly logical or scientific basis, as it only proposes a method of co-agreement (not co-reponsability) of manufacturers and consumers and an alternative logical way of thinking, or organisation of the interaction between vehicles and traffic in order to form a judgement of really justifiable needs, and to achieve a robotic private vehicle, transportation and traffic. The goal of this consideration is to establish the DIVISION of energy with the help of

  18. Sustainable urban transport development : a dynamic optimisation approach

    NARCIS (Netherlands)

    Zuidgeest, Marcus Henricus Petrus

    2005-01-01

    Current transport systems and transport planning methods and models are not necessarily compatible with the requirements of sustainable transport development. Adequate transport systems can only be obtained by use of a sustainable transport paradigm and accompanying analytical framework. Therefore,

  19. Conception of transport cask with advanced safety, aimed at transportation and storage of spent nuclear fuel of power reactors, which meets the requirements of IAEA in terms of safety and increased stability during beyond-design-basis accidents and acts of terrorism

    International Nuclear Information System (INIS)

    The report is devoted to the problem of creation of a new generation of multi-purpose universal transport cask with advanced safety, aimed at transportation and storage of spent nuclear fuel (SNF) of power reactors, which meets all requirements of IAEA in terms of safety and increased stability during beyond-design-basis accidents and acts of terrorism. Meeting all IAEA requirements in terms of safety both in normal operation conditions and accidents, as well as increased stability of transport cask (TC) with SNF under the conditions of beyond-design-basis accidents and acts of terrorism has been achieved in the design of multi-purpose universal TC due to the use of DU (depleted uranium) in it. At that, it is suggested to use DU in TC, which acts as effective gamma shield and constructional material in the form of both metallic depleted uranium and metal-ceramic mixture (cermet), based on stainless or carbon steel and DU dioxide. The metal in the cermet is chosen to optimize cask performance. The use of DU in the design of multi-purpose universal TC enables getting maximum load of the container for spent nuclear fuel when meeting IAEA requirements in terms of safety and providing increased stability of the container with SNF under conditions of beyond-design-basis accident and acts of terrorism

  20. Conception of transport cask with advanced safety, aimed at transportation and storage of spent nuclear fuel of power reactors, which meets the requirements of IAEA in terms of safety and increased stability during beyond-design-basis accidents and acts of terrorism

    Energy Technology Data Exchange (ETDEWEB)

    Il' kaev, R.I.; Matveev, V.Z.; Morenko, A.I.; Shapovalov, V.I. [Russian Federal Nuclear Center - All-Russian Research Inst. of Experimental Physics, Sarov (Russian Federation); Semenov, A.G.; Sergeyev, V.M.; Orlov, V.K. [All-Russian Research Inst. of Inorganic Materials, Moscow (Russian Federation); Shatalov, V.V.; Gotovchikov, V.T.; Seredenko, V.A. [All-Russian Research Inst. of Applied Chemistry, Moscow (Russian Federation); Haire, Jonathan M.; Forsberg, C.W. [Oak Ridge National Lab., Oak Ridge (United States)

    2004-07-01

    The report is devoted to the problem of creation of a new generation of multi-purpose universal transport cask with advanced safety, aimed at transportation and storage of spent nuclear fuel (SNF) of power reactors, which meets all requirements of IAEA in terms of safety and increased stability during beyond-design-basis accidents and acts of terrorism. Meeting all IAEA requirements in terms of safety both in normal operation conditions and accidents, as well as increased stability of transport cask (TC) with SNF under the conditions of beyond-design-basis accidents and acts of terrorism has been achieved in the design of multi-purpose universal TC due to the use of DU (depleted uranium) in it. At that, it is suggested to use DU in TC, which acts as effective gamma shield and constructional material in the form of both metallic depleted uranium and metal-ceramic mixture (cermet), based on stainless or carbon steel and DU dioxide. The metal in the cermet is chosen to optimize cask performance. The use of DU in the design of multi-purpose universal TC enables getting maximum load of the container for spent nuclear fuel when meeting IAEA requirements in terms of safety and providing increased stability of the container with SNF under conditions of beyond-design-basis accident and acts of terrorism.

  1. Transportation Business Plan

    International Nuclear Information System (INIS)

    The Transportation Business Plan is a step in the process of procuring the transportation system. It sets the context for business strategy decisions by providing pertinent background information, describing the legislation and policies governing transportation under the NWPA, and describing requirements of the transportation system. Included in the document are strategies for procuring shipping casks and transportation support services. In the spirit of the NWPA directive to utilize the private sector to the maximum extent possible, opportunities for business ventures are obvious throughout the system development cycle

  2. 现代军事物流发展对军交运输装备保障能力的需求分析%Analysis of Requirement of Military Transportation Equipment Support Capacity on Evolution of Modern Military Logistic

    Institute of Scientific and Technical Information of China (English)

    曹海泉; 崔振宇; 沈咏梅

    2012-01-01

    Military transportation equipment is the main carrier to the flow of military material in the system of modem mili- tary logistic. It is analyzed in this paper that to make overall plans and take all factors into consideration of military trans- portation equipment development, to recognize the requirement of military transportation equipment support capacity on the evolution of modem military logistic in the progress of building the system of modem military Logistic. It is vital realistic to establish countermeasure of military transportation equipment development. It will lead to efficiencies of Military Logistic.%军交运输装备是军事物流系统中军事物资流动的重要载体。在构建现代军事物流体系过程中,统筹考虑军交运输装备的建设与发展,认清现代军事物流发展对军交运输装备建设与发展的需求,对于制定军交运输装备建设与发展对策、提高军事物流效率,具有重要的现实意义。

  3. The Mitochondrial Metallochaperone SCO1 Is Required to Sustain Expression of the High-Affinity Copper Transporter CTR1 and Preserve Copper Homeostasis

    Directory of Open Access Journals (Sweden)

    Christopher J. Hlynialuk

    2015-02-01

    Full Text Available Human SCO1 fulfills essential roles in cytochrome c oxidase (COX assembly and the regulation of copper (Cu homeostasis, yet it remains unclear why pathogenic mutations in this gene cause such clinically heterogeneous forms of disease. Here, we establish a Sco1 mouse model of human disease and show that ablation of Sco1 expression in the liver is lethal owing to severe COX and Cu deficiencies. We further demonstrate that the Cu deficiency is explained by a functional connection between SCO1 and CTR1, the high-affinity transporter that imports Cu into the cell. CTR1 is rapidly degraded in the absence of SCO1 protein, and we show that its levels are restored in Sco1−/− mouse embryonic fibroblasts upon inhibition of the proteasome. These data suggest that mitochondrial signaling through SCO1 provides a post-translational mechanism to regulate CTR1-dependent Cu import into the cell, and they further underpin the importance of mitochondria in cellular Cu homeostasis.

  4. N-glycan containing a core α1,3-fucose residue is required for basipetal auxin transport and gravitropic response in rice (Oryza sativa).

    Science.gov (United States)

    Harmoko, Rikno; Yoo, Jae Yong; Ko, Ki Seong; Ramasamy, Nirmal Kumar; Hwang, Bo Young; Lee, Eun Ji; Kim, Ho Soo; Lee, Kyung Jin; Oh, Doo-Byoung; Kim, Dool-Yi; Lee, Sanghun; Li, Yang; Lee, Sang Yeol; Lee, Kyun Oh

    2016-10-01

    In plants, α1,3-fucosyltransferase (FucT) catalyzes the transfer of fucose from GDP-fucose to asparagine-linked GlcNAc of the N-glycan core in the medial Golgi. To explore the physiological significance of this processing, we isolated two Oryza sativa (rice) mutants (fuct-1 and fuct-2) with loss of FucT function. Biochemical analyses of the N-glycan structure confirmed that α1,3-fucose is missing from the N-glycans of allelic fuct-1 and fuct-2. Compared with the wild-type cv Kitaake, fuct-1 displayed a larger tiller angle, shorter internode and panicle lengths, and decreased grain filling as well as an increase in chalky grains with abnormal shape. The mutant allele fuct-2 gave rise to similar developmental abnormalities, although they were milder than those of fuct-1. Restoration of a normal tiller angle in fuct-1 by complementation demonstrated that the phenotype is caused by the loss of FucT function. Both fuct-1 and fuct-2 plants exhibited reduced gravitropic responses. Expression of the genes involved in tiller and leaf angle control was also affected in the mutants. We demonstrate that reduced basipetal auxin transport and low auxin accumulation at the base of the shoot in fuct-1 account for both the reduced gravitropic response and the increased tiller angle.

  5. N-glycan containing a core α1,3-fucose residue is required for basipetal auxin transport and gravitropic response in rice (Oryza sativa).

    Science.gov (United States)

    Harmoko, Rikno; Yoo, Jae Yong; Ko, Ki Seong; Ramasamy, Nirmal Kumar; Hwang, Bo Young; Lee, Eun Ji; Kim, Ho Soo; Lee, Kyung Jin; Oh, Doo-Byoung; Kim, Dool-Yi; Lee, Sanghun; Li, Yang; Lee, Sang Yeol; Lee, Kyun Oh

    2016-10-01

    In plants, α1,3-fucosyltransferase (FucT) catalyzes the transfer of fucose from GDP-fucose to asparagine-linked GlcNAc of the N-glycan core in the medial Golgi. To explore the physiological significance of this processing, we isolated two Oryza sativa (rice) mutants (fuct-1 and fuct-2) with loss of FucT function. Biochemical analyses of the N-glycan structure confirmed that α1,3-fucose is missing from the N-glycans of allelic fuct-1 and fuct-2. Compared with the wild-type cv Kitaake, fuct-1 displayed a larger tiller angle, shorter internode and panicle lengths, and decreased grain filling as well as an increase in chalky grains with abnormal shape. The mutant allele fuct-2 gave rise to similar developmental abnormalities, although they were milder than those of fuct-1. Restoration of a normal tiller angle in fuct-1 by complementation demonstrated that the phenotype is caused by the loss of FucT function. Both fuct-1 and fuct-2 plants exhibited reduced gravitropic responses. Expression of the genes involved in tiller and leaf angle control was also affected in the mutants. We demonstrate that reduced basipetal auxin transport and low auxin accumulation at the base of the shoot in fuct-1 account for both the reduced gravitropic response and the increased tiller angle. PMID:27241276

  6. 民用运输飞机适坠性要求浅析%Analysis of Crash-worthiness Requirements of Civil Transport Aircraft

    Institute of Scientific and Technical Information of China (English)

    范耀宇

    2014-01-01

    全面解读了民用运输飞机适坠性要求,并对CCAR/FAR25与CS25之间差异进行剖析,总结国内外相关经验,提出适坠性后续工作方向。%The paper comprehensively states the air-worthiness requirements of crash-worthiness, analyses the difference between CCAR/FAR25 and CS25 , summarizes the experience of airplane crash-worthiness in the do-mestic and over-sea, and proposes the further research domain.

  7. Difference in the wind speeds required for initiation versus continuation of sand transport on Mars: Implications for dunes and dust storms

    CERN Document Server

    Kok, Jasper F

    2010-01-01

    Much of the surface of Mars is covered by dunes, ripples, and other features formed by the blowing of sand by wind, known as saltation. In addition, saltation loads the atmosphere with dust aerosols, which dominate the Martian climate. We show here that saltation can be maintained on Mars by wind speeds an order of magnitude less than required to initiate it. We further show that the resulting hysteresis effect causes saltation to occur for much lower wind speeds than previously thought. These findings have important implications for the formation of dust storms, sand dunes, and ripples on Mars.

  8. The Bradyrhizobium japonicum Ferrous Iron Transporter FeoAB Is Required for Ferric Iron Utilization in Free Living Aerobic Cells and for Symbiosis.

    Science.gov (United States)

    Sankari, Siva; O'Brian, Mark R

    2016-07-22

    The bacterium Bradyrhizobium japonicum USDA110 does not synthesize siderophores for iron utilization in aerobic environments, and the mechanism of iron uptake within symbiotic soybean root nodules is unknown. An mbfA bfr double mutant defective in iron export and storage activities cannot grow aerobically in very high iron medium. Here, we found that this phenotype was suppressed by loss of function mutations in the feoAB operon encoding ferrous (Fe(2+)) iron uptake proteins. Expression of the feoAB operon genes was elevated under iron limitation, but mutants defective in either gene were unable to grow aerobically over a wide external ferric (Fe(3+)) iron (FeCl3) concentration range. Thus, FeoAB accommodates iron acquisition under iron limited and iron replete conditions. Incorporation of radiolabel from either (55)Fe(2+) or (59)Fe(3+) into cells was severely defective in the feoA and feoB strains, suggesting Fe(3+) reduction to Fe(2+) prior to traversal across the cytoplasmic membrane by FeoAB. The feoA or feoB deletion strains elicited small, ineffective nodules on soybean roots, containing few bacteria and lacking nitrogen fixation activity. A feoA(E40K) mutant contained partial iron uptake activity in culture that supported normal growth and established an effective symbiosis. The feoA(E40K) strain had partial iron uptake activity in situ within nodules and in isolated cells, indicating that FeoAB is the iron transporter in symbiosis. We conclude that FeoAB supports iron acquisition under limited conditions of soil and in the iron-rich environment of a symbiotic nodule. PMID:27288412

  9. A model of the accident process as a tool to develop indicators for transportation system safety and traffic risks. Paper prepared for the U.S. Department of Transportation international symposium on surface transportation system performance, workshop session `safety : performance indicators and data requirements'.

    NARCIS (Netherlands)

    Asmussen, E.

    1981-01-01

    This paper deals with the development of indicators used in controlling the safety performance of a transportation system. Because of the very large and complex nature of transportation systems, effecting any changes proceeds very slowly. In addition, changes in the control variables by countermeasu

  10. Transportation accidents

    International Nuclear Information System (INIS)

    Predicting the possible consequences of transportation accidents provides a severe challenge to an analyst who must make a judgment of the likely consequences of a release event at an unpredictable time and place. Since it is impractical to try to obtain detailed knowledge of the meteorology and terrain for every potential accident location on a route or to obtain accurate descriptions of population distributions or sensitive property to be protected (data which are more likely to be more readily available when one deals with fixed-site problems), he is constrained to make conservative assumptions in response to a demanding public audience. These conservative assumptions are frequently offset by very small source terms (relative to a fixed site) created when a transport vehicle is involved in an accident. For radioactive materials, which are the principal interest of the authors, only the most elementary models have been used for assessing the consequences of release of these materials in the transportation setting. Risk analysis and environmental impact statements frequently have used the Pasquill-Gifford/gaussian techniques for releases of short duration, which are both simple and easy to apply and require a minimum amount of detailed information. However, after deciding to use such a model, the problem of selecting what specific parameters to use in specific transportation situations still presents itself. Additional complications arise because source terms are not well characterized, release rates can be variable over short and long time periods, and mechanisms by which source aerosols become entrained in air are not always obvious. Some approaches that have been used to address these problems will be reviewed with emphasis on guidelines to avoid the Worst-Case Scenario Syndrome

  11. Bidirectional transport of motor-driven cargoes in cell: A random walk with memory

    Science.gov (United States)

    Bhat, Deepak; Gopalakrishnan, Manoj

    2013-02-01

    Motor-driven bidirectional transport inside a cell is an intriguing phenomenon where multiple dyneins and kinesins drag cargoes and deliver them to specific locations. Quite often, both anterograde (plus) and retrograde (minus) directed motors reside simultaneously on the cargo and co-ordinate their activity such that the motion of the cargo looks like spells of plus directed and minus directed segments, with pauses in between. The stochastic tug-of-war model is widely used to analyze this motion, largely studied by computer simulations. Here, we present some analytical results for this model, such as the average durations of plus and minus-run segments and tug-of-war events as well as probabilities of persistence and reversal of directions following a tug-of-war. We show that cargo motion has memory in case of 2 dyneins and 1 kinesin driving together, while it is absent when only a dynein and a kinesin is involved.

  12. Why Lead Methylammonium tri-IODIDE perovskite-based solar cells requires a mesoporous electron transporting scaffold (but not necessarily a hole conductor)

    CERN Document Server

    Edri, Eran; Henning, Alex; Mukhopadhyay, Sabyasachi; Gartsman, Konstantin; Rosenwaks, Yossi; Hodes, Gary; Cahen, David

    2014-01-01

    CH3NH3PbI3-based solar cells were characterized with electron beam-induced current (EBIC), and compared to CH3NH3PbI3-xClx ones. A spatial map of charge separation efficiency in working cells shows p-i-n structures for both thin film cells. Effective diffusion lengths, LD, (from EBIC profile) show that holes are extracted significantly more efficiently than electrons in CH3NH3PbI3, explaining why CH3NH3PbI3-based cells require mesoporous electron conductors, while CH3NH3PbI3-xClx ones, where LD values are comparable for both charge types, do not.

  13. Chlamydomonas fla mutants reveal a link between deflagellation and intraflagellar transport

    Directory of Open Access Journals (Sweden)

    Quarmby Lynne

    2003-08-01

    Full Text Available Abstract Background Cilia and flagella are often lost in anticipation of mitosis or in response to stress. There are two ways that a cell can lose its flagella: resorption or deflagellation. Deflagellation involves active severing of the axoneme at the base of the flagellum; this process is defective in Chlamydomonas fa mutants. In contrast, resorption has been thought to occur as a consequence of constitutive disassembly at the tip in the absence of continued assembly, which requires intraflagellar transport (IFT. Chlamydomonas fla mutants are unable to build and maintain flagella due to defects in IFT. Results fla10 cells, which are defective in kinesin-II, the anterograde IFT motor, resorb their flagella at the restrictive temperature (33°C, as previously reported. We find that in standard media containing ~300 microM calcium, fla10 cells lose flagella by deflagellation at 33°C. This temperature-induced deflagellation of a fla mutant is not predicted by the IFT-based model for flagellar length control. Other fla mutants behave similarly, losing their flagella by deflagellation instead of resorption, if adequate calcium is available. These data suggest a new model whereby flagellar resorption involves active disassembly at the base of the flagellum via a mechanism with components in common with the severing machinery of deflagellation. As predicted by this model, we discovered that deflagellation stimuli induce resorption if deflagellation is blocked either by mutation in a FA gene or by lack of calcium. Further support for this model comes from our discovery that fla10-fa double mutants resorb their flagella more slowly than fla10 mutants. Conclusions Deflagellation of the fla10 mutant at the restrictive temperature is indicative of an active disassembly signal, which can manifest as either resorption or deflagellation. We propose that when IFT is halted by either an inactivating mutation or a cellular signal, active flagellar disassembly

  14. Transport policy

    OpenAIRE

    1980-01-01

    Transport is a fundamental component of all modern economies. Transport Policy presents a wide ranging collection of previously published articles which aim to provide the reader with an understanding of the main elements of transport policy.

  15. Application of stepping DSA technique in lower limb venography anterograde%步进式DSA技术在下肢顺行性静脉造影中的应用

    Institute of Scientific and Technical Information of China (English)

    陈燕; 黄健

    2011-01-01

    目的 评价步进式DSA技术在下肢顺行性静脉造影中的应用价值.方法 将要求行下肢静脉造影患者40例随机分为2组:研究组20例行步进式DSA造影,对照组20例行传统分段式DSA造影.由介入科主治以上医生及技师共同将图像满意率、造影耗时、吸收辐射剂量(DAP)、造影剂用量等进行比较评价.结果 步进式DSA造影与传统分段式DSA造影相比较,2种方法造影图像质量相仿,但步进式DSA造影对观察由下而上血流的连续性,血流动态流向,判断静脉的解剖形态及病理血管血流状况,图像更加富有整体性,一次性成功率85%(17/20).其次吸收辐射剂量,造影剂用量,总体检查时间均明显减少.结论 对下肢静脉病变,步进式DSA造影较传统分段式DSA造影环节更为简化,图像整体观及实用性强,对下肢静脉病变介入治疗方案的决策以及外科手术有很重要的客观指引作用.%Objective To investigate the value of the application of step - DSA in lower extremity venography anterograde. Methods Forty patients of lower limb venography were randomly divided into two groups: the research group of 20 cases using step - DSA and the compared group of 20 using the traditional sub - type DSA. Then the attending doctors and technicians in Invasive Technology Department had a comparison and assessment in the aspects of the image satisfaction rate, DSA time -consuming, dose area product(DAP) , dosage of contrast agent and so on. Results The DSA image quality of the two methods was similar, but compared with the traditional sub - type DSA, the stepping DSA has a better image and success rate of 85% (17/20) at the first treatment through observing the continuity of bottom - up bloodstream, dynamic flow of blood and determining the venous anatomical form and pathological blood flow conditions. Furthermore, with stepping DSA, the absorbed radiation dose, dosage of contrast agent and the total inspection time have

  16. Radiation Transport

    Energy Technology Data Exchange (ETDEWEB)

    Urbatsch, Todd James [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

    2015-06-15

    We present an overview of radiation transport, covering terminology, blackbody raditation, opacities, Boltzmann transport theory, approximations to the transport equation. Next we introduce several transport methods. We present a section on Caseology, observing transport boundary layers. We briefly broach topics of software development, including verification and validation, and we close with a section on high energy-density experiments that highlight and support radiation transport.

  17. 49 CFR 130.21 - Packaging requirements.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 2 2010-10-01 2010-10-01 false Packaging requirements. 130.21 Section 130.21 Transportation Other Regulations Relating to Transportation PIPELINE AND HAZARDOUS MATERIALS SAFETY... Packaging requirements. Each packaging used for the transportation of oil subject to this part must...

  18. HMPT: Basic Radioactive Material Transportation

    Energy Technology Data Exchange (ETDEWEB)

    Hypes, Philip A. [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

    2016-02-29

    Hazardous Materials and Packaging and Transportation (HMPT): Basic Radioactive Material Transportation Live (#30462, suggested one time) and Test (#30463, required initially and every 36 months) address the Department of Transportation’s (DOT’s) function-specific [required for hazardous material (HAZMAT) handlers, packagers, and shippers] training requirements of the HMPT Los Alamos National Laboratory (LANL) Labwide training. This course meets the requirements of 49 CFR 172, Subpart H, Section 172.704(a)(ii), Function-Specific Training.

  19. Energy and transport.

    Science.gov (United States)

    Woodcock, James; Banister, David; Edwards, Phil; Prentice, Andrew M; Roberts, Ian

    2007-09-22

    We examine the links between fossil-fuel-based transportation, greenhouse-gas emissions, and health. Transport-related carbon emissions are rising and there is increasing consensus that the growth in motorised land vehicles and aviation is incompatible with averting serious climate change. The energy intensity of land transport correlates with its adverse health effects. Adverse health effects occur through climate change, road-traffic injuries, physical inactivity, urban air pollution, energy-related conflict, and environmental degradation. For the world's poor people, walking is the main mode of transport, but such populations often experience the most from the harms of energy-intensive transport. New energy sources and improvements in vehicle design and in information technology are necessary but not sufficient to reduce transport-related carbon emissions without accompanying behavioural change. By contrast, active transport has the potential to improve health and equity, and reduce emissions. Cities require safe and pleasant environments for active transport with destinations in easy reach and, for longer journeys, public transport that is powered by renewable energy, thus providing high levels of accessibility without car use. Much investment in major road projects does not meet the transport needs of poor people, especially women whose trips are primarily local and off road. Sustainable development is better promoted through improving walking and cycling infrastructures, increasing access to cycles, and investment in transport services for essential needs. Our model of London shows how increased active transport could help achieve substantial reductions in emissions by 2030 while improving population health. There exists the potential for a global contraction and convergence in use of fossil-fuel energy for transport to benefit health and achieve sustainability. PMID:17868817

  20. Carótida comum como via de estabelecimento da circulação extracorpórea para perfusão sistêmica e cerebral seletiva na correção das doenças da aorta Carotid artery inflow and anterograde cerebral perfusion in operations to correct aortic disease

    Directory of Open Access Journals (Sweden)

    Luiz Henrique Pamplona Corte Real de Carvalho

    2006-09-01

    Full Text Available OBJETIVO: Analisar os resultados da utilização da artéria carótida comum direita no estabelecimento da circulação extracorpórea (CEC para perfusão sistêmica (PS, bem como na utilização como via anterógrada para proteção cerebral (PC, nos pacientes submetidos à correção das doenças que envolveram a aorta ascendente (AA. MÉTODO: Foram operados 23 pacientes portadores de várias afecções da AA, nos quais a abordagem foi possível por meio do estabelecimento da CEC pela anastomose de um tubo de PTFE (politetrafluoretileno à artéria carótida comum direita e manutenção de fluxo cerebral anterógrado durante a confecção da anastomose distal. O tempo médio de CEC foi de 195 minutos (152 a 253 minutos, a temperatura média sistêmica foi de 24ºC (18ºC a 25ºC, hipofluxo cerebral anterógrado, com média de 29 minutos (27 a 51 minutos e o tempo de internação média foi de 18 dias (8 a 30 dias. RESULTADOS: Todos os pacientes despertaram nas primeiras 48 horas sem dano neurológico. Por ocasião da pré-alta, foram submetidos ao teste MINI MENTAL STATE EXAMINATION (MMSE, que demonstrou não haver desordens cognitivas com dano neurológico. Ocorreram dois casos de infecção pulmonar, dois pacientes necessitaram de diálise e ocorreram dois óbitos relacionados à gravidade dos casos. Não houve óbito per-operatório. CONCLUSÃO: Este é um método prático, rápido, seguro, eficaz e reprodutível, tanto no estabelecimento da CEC como da proteção cerebral anterógrada, reduzindo o risco de isquemia cerebral, alterando a história natural da evolução pós-operatória destas correções.OBJECTIVE: To evaluate the use of the right common carotid artery to establish cardiopulmonary bypass (CPB and the use of anterograde cerebral perfusion as cerebral protection in patients scheduled for ascending aortic repair surgery. METHOD: Twenty-three patients were submitted to aortic repair because of different aortic diseases. In all

  1. Neurotransmitter transporters

    DEFF Research Database (Denmark)

    Gether, Ulrik; Andersen, Peter H; Larsson, Orla M;

    2006-01-01

    The concentration of neurotransmitters in the extracellular space is tightly controlled by distinct classes of membrane transport proteins. This review focuses on the molecular function of two major classes of neurotransmitter transporter that are present in the cell membrane of neurons and....../or glial cells: the solute carrier (SLC)1 transporter family, which includes the transporters that mediate the Na(+)-dependent uptake of glutamate, and the SLC6 transporter family, which includes the transporters that mediate the Na(+)-dependent uptake of dopamine, 5-HT, norepinephrine, glycine and GABA....... Recent research has provided substantial insight into the structure and function of these transporters. In particular, the recent crystallizations of bacterial homologs are of the utmost importance, enabling the first reliable structural models of the mammalian neurotransmitter transporters...

  2. School Transportation.

    Science.gov (United States)

    Executive Educator, 1990

    1990-01-01

    This special section on student transportation offers a case study of a school system that recycles buses for safety drills; articles on fuel-saving strategies, the pros and cons of contracting for transportation services or operating a publicly owned bus fleet, and advice on full cost accounting for transportation costs; and a transportation…

  3. Disruption of mitochondrial DNA replication in Drosophila increases mitochondrial fast axonal transport in vivo.

    Directory of Open Access Journals (Sweden)

    Rehan M Baqri

    Full Text Available Mutations in mitochondrial DNA polymerase (pol gamma cause several progressive human diseases including Parkinson's disease, Alper's syndrome, and progressive external ophthalmoplegia. At the cellular level, disruption of pol gamma leads to depletion of mtDNA, disrupts the mitochondrial respiratory chain, and increases susceptibility to oxidative stress. Although recent studies have intensified focus on the role of mtDNA in neuronal diseases, the changes that take place in mitochondrial biogenesis and mitochondrial axonal transport when mtDNA replication is disrupted are unknown. Using high-speed confocal microscopy, electron microscopy and biochemical approaches, we report that mutations in pol gamma deplete mtDNA levels and lead to an increase in mitochondrial density in Drosophila proximal nerves and muscles, without a noticeable increase in mitochondrial fragmentation. Furthermore, there is a rise in flux of bidirectional mitochondrial axonal transport, albeit with slower kinesin-based anterograde transport. In contrast, flux of synaptic vesicle precursors was modestly decreased in pol gamma-alpha mutants. Our data indicate that disruption of mtDNA replication does not hinder mitochondrial biogenesis, increases mitochondrial axonal transport, and raises the question of whether high levels of circulating mtDNA-deficient mitochondria are beneficial or deleterious in mtDNA diseases.

  4. The Kiss-and-Run Model of Intra-Golgi Transport

    Directory of Open Access Journals (Sweden)

    Alexander A. Mironov

    2012-06-01

    Full Text Available The Golgi apparatus (GA is the main station along the secretory pathway. Mechanisms of intra-Golgi transport remain unresolved. Three models compete with each other for the right to be defined as the paradigm. The vesicular model cannot explain the following: (1 lipid droplets and aggregates of procollagen that are larger than coatomer I (COPI-dependent vesicles are transported across the GA; and (2 most anterograde cargoes are depleted in COPI vesicles. The compartment progression/maturation model has the following problems: (1 most Golgi-resident proteins are depleted in COPI vesicles; (2 there are no COPI vesicles for the recycling of the resident proteins in the trans-most-Golgi cisterna; and (3 different proteins have different rates of intra-Golgi transport. The diffusion model based on permanent inter-cisternal connections cannot explain the existence of lipid, ionic and protein gradients across the Golgi stacks. In contrast, the kiss-and-run model has the potential to explain most of the experimental observations. The kiss-and-run model can be symmetric when fusion and then fission occurs in the same place, and asymmetric when fusion takes place in one location, whereas fission takes place in another. The asymmetric kiss-and-run model resembles the carrier maturation mechanism, and it can be used to explain the transport of large cargo aggregates.

  5. Transcriptional profiling of C. elegans DAF-19 uncovers a ciliary base-associated protein and a CDK/CCRK/LF2p-related kinase required for intraflagellar transport.

    Science.gov (United States)

    Phirke, Prasad; Efimenko, Evgeni; Mohan, Swetha; Burghoorn, Jan; Crona, Filip; Bakhoum, Mathieu W; Trieb, Maria; Schuske, Kim; Jorgensen, Erik M; Piasecki, Brian P; Leroux, Michel R; Swoboda, Peter

    2011-09-01

    Cilia are ubiquitous cell surface projections that mediate various sensory- and motility-based processes and are implicated in a growing number of multi-organ genetic disorders termed ciliopathies. To identify new components required for cilium biogenesis and function, we sought to further define and validate the transcriptional targets of DAF-19, the ciliogenic C. elegans RFX transcription factor. Transcriptional profiling of daf-19 mutants (which do not form cilia) and wild-type animals was performed using embryos staged to when the cell types developing cilia in the worm, the ciliated sensory neurons (CSNs), still differentiate. Comparisons between the two populations revealed 881 differentially regulated genes with greater than a 1.5-fold increase or decrease in expression. A subset of these was confirmed by quantitative RT-PCR. Transgenic worms expressing transcriptional GFP fusions revealed CSN-specific expression patterns for 11 of 14 candidate genes. We show that two uncharacterized candidate genes, termed dyf-17 and dyf-18 because their corresponding mutants display dye-filling (Dyf) defects, are important for ciliogenesis. DYF-17 localizes at the base of cilia and is specifically required for building the distal segment of sensory cilia. DYF-18 is an evolutionarily conserved CDK7/CCRK/LF2p-related serine/threonine kinase that is necessary for the proper function of intraflagellar transport, a process critical for cilium biogenesis. Together, our microarray study identifies targets of the evolutionarily conserved RFX transcription factor, DAF-19, providing a rich dataset from which to uncover-in addition to DYF-17 and DYF-18-cellular components important for cilium formation and function. PMID:21740898

  6. Sustainable Transportation

    DEFF Research Database (Denmark)

    Hall, Ralph P.; Gudmundsson, Henrik; Marsden, Greg;

    2014-01-01

    The transportation system is the backbone of economic and social progress and the means by which humans access goods and services and connect with one another. Yet, as the scale of transportation activities has grown worldwide, so too have the negative environmental, social, and economic impacts...... that relate to the construction and maintenance of transportation infrastructure and the operation or use of the different transportation modes. The concept of sustainable transportation emerged in response to these concerns as part of the broader notion of sustainable development. Given the transportation...... sector’s significant contribution to global challenges such as climate change, it is often said that sustainable development cannot be achieved without sustainable transportation....

  7. Crew Transportation Technical Management Processes

    Science.gov (United States)

    Mckinnie, John M. (Compiler); Lueders, Kathryn L. (Compiler)

    2013-01-01

    Under the guidance of processes provided by Crew Transportation Plan (CCT-PLN-1100), this document, with its sister documents, International Space Station (ISS) Crew Transportation and Services Requirements Document (CCT-REQ-1130), Crew Transportation Technical Standards and Design Evaluation Criteria (CCT-STD-1140), Crew Transportation Operations Standards (CCT STD-1150), and ISS to Commercial Orbital Transportation Services Interface Requirements Document (SSP 50808), provides the basis for a National Aeronautics and Space Administration (NASA) certification for services to the ISS for the Commercial Provider. When NASA Crew Transportation System (CTS) certification is achieved for ISS transportation, the Commercial Provider will be eligible to provide services to and from the ISS during the services phase.

  8. Robotic transportation.

    Science.gov (United States)

    Lob, W S

    1990-09-01

    Mobile robots perform fetch-and-carry tasks autonomously. An intelligent, sensor-equipped mobile robot does not require dedicated pathways or extensive facility modification. In the hospital, mobile robots can be used to carry specimens, pharmaceuticals, meals, etc. between supply centers, patient areas, and laboratories. The HelpMate (Transitions Research Corp.) mobile robot was developed specifically for hospital environments. To reach a desired destination, Help-Mate navigates with an on-board computer that continuously polls a suite of sensors, matches the sensor data against a pre-programmed map of the environment, and issues drive commands and path corrections. A sender operates the robot with a user-friendly menu that prompts for payload insertion and desired destination(s). Upon arrival at its selected destination, the robot prompts the recipient for a security code or physical key and awaits acknowledgement of payload removal. In the future, the integration of HelpMate with robot manipulators, test equipment, and central institutional information systems will open new applications in more localized areas and should help overcome difficulties in filling transport staff positions. PMID:2208684

  9. Robotic transportation.

    Science.gov (United States)

    Lob, W S

    1990-09-01

    Mobile robots perform fetch-and-carry tasks autonomously. An intelligent, sensor-equipped mobile robot does not require dedicated pathways or extensive facility modification. In the hospital, mobile robots can be used to carry specimens, pharmaceuticals, meals, etc. between supply centers, patient areas, and laboratories. The HelpMate (Transitions Research Corp.) mobile robot was developed specifically for hospital environments. To reach a desired destination, Help-Mate navigates with an on-board computer that continuously polls a suite of sensors, matches the sensor data against a pre-programmed map of the environment, and issues drive commands and path corrections. A sender operates the robot with a user-friendly menu that prompts for payload insertion and desired destination(s). Upon arrival at its selected destination, the robot prompts the recipient for a security code or physical key and awaits acknowledgement of payload removal. In the future, the integration of HelpMate with robot manipulators, test equipment, and central institutional information systems will open new applications in more localized areas and should help overcome difficulties in filling transport staff positions.

  10. Transport of sugars.

    Science.gov (United States)

    Chen, Li-Qing; Cheung, Lily S; Feng, Liang; Tanner, Widmar; Frommer, Wolf B

    2015-01-01

    Soluble sugars serve five main purposes in multicellular organisms: as sources of carbon skeletons, osmolytes, signals, and transient energy storage and as transport molecules. Most sugars are derived from photosynthetic organisms, particularly plants. In multicellular organisms, some cells specialize in providing sugars to other cells (e.g., intestinal and liver cells in animals, photosynthetic cells in plants), whereas others depend completely on an external supply (e.g., brain cells, roots and seeds). This cellular exchange of sugars requires transport proteins to mediate uptake or release from cells or subcellular compartments. Thus, not surprisingly, sugar transport is critical for plants, animals, and humans. At present, three classes of eukaryotic sugar transporters have been characterized, namely the glucose transporters (GLUTs), sodium-glucose symporters (SGLTs), and SWEETs. This review presents the history and state of the art of sugar transporter research, covering genetics, biochemistry, and physiology-from their identification and characterization to their structure, function, and physiology. In humans, understanding sugar transport has therapeutic importance (e.g., addressing diabetes or limiting access of cancer cells to sugars), and in plants, these transporters are critical for crop yield and pathogen susceptibility.

  11. E-learning for Transport Security

    International Nuclear Information System (INIS)

    In this course we will look at how to ensure nuclear and radioactive material is transported safely and securely through the following topics: Objectives of transport security; International and national requirements, recommendations and guidance; Background of safety regulations; Basic principles and fundamentals; Application of security functions; Transport security technologies; Transport security plans, readiness reviews and corrective actions

  12. 45 CFR 1310.23 - Coordinated transportation.

    Science.gov (United States)

    2010-10-01

    ... 45 Public Welfare 4 2010-10-01 2010-10-01 false Coordinated transportation. 1310.23 Section 1310... START PROGRAM HEAD START TRANSPORTATION Special Requirements § 1310.23 Coordinated transportation. (a) Each agency providing transportation services must make reasonable efforts to coordinate...

  13. An Overview of Airworthiness Requirements for Transport Category Airplanes to Prevent Widespread Fatigue Damage%运输类飞机防止广布疲劳损伤的适航要求

    Institute of Scientific and Technical Information of China (English)

    李亚智; 李强; 沈培良

    2011-01-01

    Significant changes have arisen recently in FAA standards for the continued airworthiness and operations of transport category airplanes, which brings new challenge to the developing large Chinese airliner applying for type certification. The background of widespread fatigue damage (WFD) requirements in airworthiness regulation for transport category airplanes metallic structures are introduced and the procedure of the WFD assessment for the new type designs and the determination of their operational limits, inspection thresholds and maintenance tasks are described. The author is also sharing his understanding to the WFD concept and the relationship between WFD evaluation and fatigue/damage tolerance evaluation. Suggestions are put forward to enhance the research of the testing and analytical means as well as the engineering development for the WFD evaluation, in order to provide evidence and basis for the large civil aircraft design demonstrating compliance to applicable regulations.%近年来,运输类飞机适航条例围绕飞机的持续适航和使用要求发生了许多重要改变,给我国发展中的大型客机的适航审定带来新的课题。为此,本文首先介绍了适航条例关于运输类飞机金属结构广布疲劳损伤(WFD)要求的演变背景;然后,根据适航条例的最新修正案和咨询通报最新要求,阐述了新设计飞机的WFD评定、使用限制、检查门槛值和维修措施的制定步骤,并且提出了对WFD的概念及WFD评定与疲劳/损伤容限评定的关系的理解和思考,建议国内加强WFD评定的学术和工程发展研究,为大型客机设计和适航符合性验证提供依据。

  14. 49 CFR 176.89 - Control of transport vehicles.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 2 2010-10-01 2010-10-01 false Control of transport vehicles. 176.89 Section 176... Special Requirements for Transport Vehicles Loaded With Hazardous Materials and Transported on Board Ferry Vessels § 176.89 Control of transport vehicles. (a) A transport vehicle containing hazardous materials...

  15. Sediment Transport

    DEFF Research Database (Denmark)

    Liu, Zhou

    Flow and sediment transport are important in relation to several engineering topics, e.g. erosion around structures, backfilling of dredged channels and nearshore morphological change. The purpose of the present book is to describe both the basic hydrodynamics and the basic sediment transport...... mechanics. Chapter 1 deals with fundamentals in fluid mechanics with emphasis on bed shear stress by currents, while chapter 3 discusses wave boundary layer theory. They are both written with a view to sediment transport. Sediment transport in rivers, cross-shore and longshore are dealt with in chapters 2...

  16. Quality Assurance Plan for Transportation Management Division Transportation Training Programs

    International Nuclear Information System (INIS)

    The U.S. Department of Transportation (DOT) implemented new rules requiring minimum levels of training for certain key individuals who handle, package, transport, or otherwise prepare hazardous materials for transportation. In response to these rules, the U.S. Department of Energy (DOE), Transportation Management Division (TMD), has developed a transportation safety training program. This program supplies designed instructional methodology and course materials to provide basic levels of DOT training to personnel for whom training has become mandatory. In addition, this program provides advanced hazardous waste and radioactive material packaging and transportation training to help personnel achieve proficiency and/or certification as hazardous waste and radioactive material shippers. This training program does not include site-specific or task-specific training beyond DOT requirements

  17. Maritime Transport

    OpenAIRE

    Veenstra, A.W.

    2002-01-01

    This important volume brings together an authoritative selection of the leading papers on the subject of maritime transport. With a new introductory essay by the editors, the collection provides a thorough examination of the topics associated with this area, including maritime economics, transport law and policy.

  18. Eligibility Requirements

    Science.gov (United States)

    ... Home > Donating Blood > Eligibility Requirements Printable Version Eligibility Requirements This page uses Javascript. Your browser either doesn' ... donors » Weigh at least 110 lbs. Additional weight requirements apply for donors 18-years-old and younger ...

  19. [Recommendations for neonatal transport].

    Science.gov (United States)

    Moreno Hernando, J; Thió Lluch, M; Salguero García, E; Rite Gracia, S; Fernández Lorenzo, J R; Echaniz Urcelay, I; Botet Mussons, F; Herranz Carrillo, G; Sánchez Luna, M

    2013-08-01

    During pregnancy, it is not always possible to identify maternal or foetal risk factors. Infants requiring specialised medical care are not always born in centres providing intensive care and will need to be transferred to a referral centre where intensive care can be provided. Therefore Neonatal Transport needs to be considered as part of the organisation of perinatal health care. The aim of Neonatal Transport is to transfer a newborn infant requiring intensive care to a centre where specialised resources and experience can be provided for the appropriate assessment and continuing treatment of a sick newborn infant. Intrauterine transfer is the ideal mode of transport when the birth of an infant with risk factors is diagnosed. Unfortunately, not all problems can be detected in advance with enough time to safely transfer a pregnant woman. Around 30- 50% of risk factors will be diagnosed during labour or soon after birth. Therefore, it is important to have the knowledge and resources to resuscitate and stabilise a newborn infant, as well as a specialised neonatal transport system. With this specialised transport it is possible to transfer newly born infants with the same level of care that they would receive if they had been born in a referral hospital, without increasing their risks or affecting the wellbeing of the newborn. The Standards Committee of the Spanish Society of Neonatology reviewed and updated recommendations for intrauterine transport and indications for neonatal transfer. They also reviewed organisational and logistic factors involved with performing neonatal transport. The Committee review included the type of personnel who should be involved; communication between referral and receiving hospitals; documentation; mode of transport; equipment to stabilise newly born infants; management during transfer, and admission at the referral hospital.

  20. School Transportation: Administrator's Handbook

    Science.gov (United States)

    Missouri Department of Elementary and Secondary Education, 2008

    2008-01-01

    Pupil transportation is an essential part of the overall school program, which requires constant supervision and direction. Perhaps no other phase of the school program is more closely observed by the public or has a greater tendency to mold public opinion about the schools than the school bus system. The success of any school district pupil…

  1. Goods Transport Modelling, Vol 1

    DEFF Research Database (Denmark)

    Petersen, Morten Steen (red.); Kristiansen, Jørgen

    The report is a study of data requirements and methodologies for goods transport. The study is intended to provide the basis for general discussion about the application of goods transport models in Denmark. The report provides an overview of different types of models and data availability....

  2. Transport service

    CERN Multimedia

    C. Cerruti / FI

    2006-01-01

    A large number of pallet-crates (panières grillagées), which are used for transporting equipment and for removals, have been dispatched to various locations around the CERN site. We kindly request all users who may have such crates in their possession and no longer need them to make the necessary arrangements (EDH request to the Transport Group) to return them to Building 133, as we currently have no more in stock. Claude CERRUTI / FI-PI

  3. Transporting Forensic Psychiatric Patients.

    Science.gov (United States)

    Dike, Charles C; Nicholson, Elizabeth; Young, John L

    2015-12-01

    Patients in a forensic psychiatric facility often require escorted transport to medical facilities for investigations or treatments of physical health ailments. Transporting these patients presents significant safety and custody challenges because of the nature of patients housed in forensic psychiatric facilities. A significant proportion of these patients may be transfers from the Department of Corrections (DOC) under legal mandates for psychiatric evaluation and treatment better provided in a hospital setting, and most of them will return to the DOC. Although departments of correction have protocols for escorting these potentially dangerous individuals, it is unclear whether receiving psychiatric hospitals have established procedures for maintaining the safety of others and custody of these individuals during transportation outside the hospital facility. The literature is sparse on precautions to be observed when transporting dangerous forensic psychiatric patients, including those with high escape risk. In this article, we describe one forensic inpatient facility's procedure for determining the appropriate level needed to transport these individuals outside of the forensic facility. We also describe the risk assessment procedure for determining level of transport. These are quality improvement measures resulting from a critical review of an incident of escape from the forensic facility several years ago. PMID:26668224

  4. Conceptual design for HIC transport package

    International Nuclear Information System (INIS)

    Korea Hydro and Nuclear Power Co. Ltd.(KHNP) is developing a HIC(High Integrity Container) transport package to transport a high integrity container from the radioactive waste treatment facility of the nuclear power plant to the interim storage building of radioactive waste. The transport package is designed according to the requirements of the Korean and the IAEA transport regulations for Type B package. The transport package provides containment, radiation shielding, structural integrity and passive heat removal for normal transport and hypothetical accident conditions. This paper describes the conceptual design for the HIC transport package

  5. Defense TRU waste transportation and receiving simulation

    International Nuclear Information System (INIS)

    Pacific Northwest Laboratory has recently conducted a simulation analysis to predict the transportation and waste handling equipment requirements for transporting government-owned transuranic waste to the Waste Isolation Pilot Plant (WIPP). Two interrelated models were developed. One estimates the time required to receive and unload shipping containers at the WIPP. The other model uses this estimate to predict the number of shipping containers and transporters that will be required

  6. Onsite transportation hazards assessment

    International Nuclear Information System (INIS)

    This report documents the emergency preparedness Hazards Assessment for the onsite transportation of hazardous material at the Hanford Site. The assessment is required by US Department of Energy (DOE) Order 5500.3A and provides the technical basis for the emergency classification and response procedures. A distinction is made between onsite for the purpose of emergency preparedness and onsite for the purpose of applying US Department of Transportation (DOT) regulations. Onsite for the purpose of emergency preparedness is considered to be within the physical boundary of the entire Hanford Site. Onsite for the purpose of applying DOT regulations is north of the Wye Barricade

  7. Transport Safety Performance Indicators

    OpenAIRE

    WEGMAN, F.; Brouwer, M.; CAUZARD, J.P; Elvik, R.; MACHATA, K.; Makinen, T.; STENBORG, L.; Yannis, G.

    2001-01-01

    Transport safety is a key priority in the Common Transport Policy and a high level of protection for EU citizens in all harmonisation activity is required by the Treaty. Policymakers and managers aiming for a higher level of safety need to take an interest in as many of the factors influencing safety as possible and, at least, those factors they are able to affect or control. Safety performance indicators provide a means by which policymakers can ensure that their actions are as effective as ...

  8. 49 CFR 563.6 - Requirements for vehicles.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 6 2010-10-01 2010-10-01 false Requirements for vehicles. 563.6 Section 563.6 Transportation Other Regulations Relating to Transportation (Continued) NATIONAL HIGHWAY TRAFFIC SAFETY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION EVENT DATA RECORDERS § 563.6 Requirements for vehicles. Each...

  9. Changing the Cultural Paradigm to Meet Emerging Requirements

    Science.gov (United States)

    Robbins, William W.

    2007-01-01

    This viewgraph presentation reviews changes that are required in Space Transportation. This new transportation paradigm from the reliance on the Space Shuttle to a mixture of space transportation vehicles, i.e., the Russian Progress, vehicle, the Japanese HTV, the European ATV and other commercial orbital transportation systems, requires a new cultural paradigm. This new paradigm is outlined, and reviewed.

  10. Stochastic models of intracellular transport

    KAUST Repository

    Bressloff, Paul C.

    2013-01-09

    The interior of a living cell is a crowded, heterogenuous, fluctuating environment. Hence, a major challenge in modeling intracellular transport is to analyze stochastic processes within complex environments. Broadly speaking, there are two basic mechanisms for intracellular transport: passive diffusion and motor-driven active transport. Diffusive transport can be formulated in terms of the motion of an overdamped Brownian particle. On the other hand, active transport requires chemical energy, usually in the form of adenosine triphosphate hydrolysis, and can be direction specific, allowing biomolecules to be transported long distances; this is particularly important in neurons due to their complex geometry. In this review a wide range of analytical methods and models of intracellular transport is presented. In the case of diffusive transport, narrow escape problems, diffusion to a small target, confined and single-file diffusion, homogenization theory, and fractional diffusion are considered. In the case of active transport, Brownian ratchets, random walk models, exclusion processes, random intermittent search processes, quasi-steady-state reduction methods, and mean-field approximations are considered. Applications include receptor trafficking, axonal transport, membrane diffusion, nuclear transport, protein-DNA interactions, virus trafficking, and the self-organization of subcellular structures. © 2013 American Physical Society.

  11. Initiatives in transport cask license

    Energy Technology Data Exchange (ETDEWEB)

    Patterson, John [NAC International, Aiken, SC (United States). Foreign Research Reactor Liaison]. E-mail: nacaiken@aol.com

    1998-07-01

    The variations in research reactor fuel form, configuration, irradiation characteristics, and transport cask have required a substantial number of transport cask licensing actions associated with foreign research reactor spent fuel transportation. When compounded by limited time for shipment preparations, due to contract timing or delayed receipt of technical data, the number and timing of certifications has adversely impacted the ability of regulatory agencies to support intended shipping schedules. This issue was brought into focus at a april, 1998 meeting among DOE, the US Nuclear Regulatory Commission, and DOE's spent fuel transportation contractors. (author)

  12. Air transport system

    CERN Document Server

    Schmitt, Dieter

    2016-01-01

    The book addresses all major aspects to be considered for the design and operation of aircrafts within the entire transportation chain. It provides the basic information about the legal environment, which defines the basic requirements for aircraft design and aircraft operation. The interactions between  airport, air traffic management and the airlines are described. The market forecast methods and the aircraft development process are explained to understand the very complex and risky business of an aircraft manufacturer. The principles of flight physics as basis for aircraft design are presented and linked to the operational and legal aspects of air transport including all environmental impacts. The book is written for graduate students as well as for engineers and experts, who are working in aerospace industry, at airports or in the domain of transport and logistics.

  13. 49 CFR 569.7 - Requirements.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 6 2010-10-01 2010-10-01 false Requirements. 569.7 Section 569.7 Transportation Other Regulations Relating to Transportation (Continued) NATIONAL HIGHWAY TRAFFIC SAFETY ADMINISTRATION... examination of the tire either mounted on the rim, or dismounted, whichever is applicable: (A) Cracking...

  14. Infrastructure Requirements for an Expanded Fuel Ethanol Industry

    Energy Technology Data Exchange (ETDEWEB)

    Reynolds, Robert E. [Downstream Alternatives, Inc., South Bend, IN (United States)

    2002-01-15

    This report provides technical information specifically related to ethanol transportation, distribution, and marketing issues. This report required analysis of the infrastructure requirements for an expanded ethanol industry.

  15. Sustainable Transport

    OpenAIRE

    Webber, Melvin

    2006-01-01

    I assume we’ll want to sustain any mode of transport only if we judge it to be effective and desirable, and of course, only if we think we can afford to sustain it. Over time, we’ve abandoned any number of modes that failed those tests — horsecars, trolleycars, and pullmancars, among others; and we’ve kept those that passed the tests — most notably motorcars, airplanes, and ships. In retrospect, it seems we’ve been pretty draconian in rejecting transport modes that have failed in the market p...

  16. Single-particle tracking uncovers dynamics of glutamate-induced retrograde transport of NF-κB p65 in living neurons.

    Science.gov (United States)

    Widera, Darius; Klenke, Christin; Nair, Deepak; Heidbreder, Meike; Malkusch, Sebastian; Sibarita, Jean-Baptiste; Choquet, Daniel; Kaltschmidt, Barbara; Heilemann, Mike; Kaltschmidt, Christian

    2016-10-01

    Retrograde transport of NF-κB from the synapse to the nucleus in neurons is mediated by the dynein/dynactin motor complex and can be triggered by synaptic activation. The caliber of axons is highly variable ranging down to 100 nm, aggravating the investigation of transport processes in neurites of living neurons using conventional light microscopy. We quantified for the first time the transport of the NF-κB subunit p65 using high-density single-particle tracking in combination with photoactivatable fluorescent proteins in living mouse hippocampal neurons. We detected an increase of the mean diffusion coefficient ([Formula: see text]) in neurites from [Formula: see text] to [Formula: see text] after stimulation with glutamate. We further observed that the relative amount of retrogradely transported p65 molecules is increased after stimulation. Glutamate treatment resulted in an increase of the mean retrograde velocity from [Formula: see text] to [Formula: see text], whereas a velocity increase from [Formula: see text] to [Formula: see text] was observed for anterogradely transported p65. This study demonstrates for the first time that glutamate stimulation leads to an increased mobility of single NF-κB p65 molecules in neurites of living hippocampal neurons. PMID:27226975

  17. Transport Technologies and Policy Scenarios to 2050

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2007-10-15

    As part of the major WEC study on Scenarios to 2050, a specific investigation was undertaken on measures required in the transport sector to secure sustainable energy and sustainable mobility in the future. This report outlines the results conducted by a study group of international WEC transport experts and gives concrete policy recommendations to develop sustainable transport systems.

  18. 42 CFR 9.11 - Animal transport.

    Science.gov (United States)

    2010-10-01

    ... CHIMPANZEES HELD IN THE FEDERALLY SUPPORTED SANCTUARY SYSTEM § 9.11 Animal transport. The transportation of chimpanzees by surface or air must be in accordance with the requirements set forth in the Animal Welfare Act... 42 Public Health 1 2010-10-01 2010-10-01 false Animal transport. 9.11 Section 9.11 Public...

  19. Power requirements for PHERB powertrain

    Science.gov (United States)

    Norbakyah, J. S.; Atiq, W. H.; Salisa, A. R.

    2015-12-01

    Boats are considered as favourite maritime transportation designed for recreation activities, fishing and surveillance purposes. However, in tropical developed countries, boats are employed for different applications such as passenger and goods transportation. In this paper, the power requirements for a proposed plug-in hybrid electric recreational boat (PHERB) powertrain is determined using a steady state velocity and the Kuala Terengganu river driving cycle according to the boat parameters, specifications and performance requirements. The boat power requirements can be used to size the main components for PHERB powertrain. The results obtained from this analysis are within reasonable range and satisfactory.

  20. Bioreactor Mass Transport Studies

    Science.gov (United States)

    Kleis, Stanley J.; Begley, Cynthia M.

    1997-01-01

    The objectives of the proposed research efforts were to develop both a simulation tool and a series of experiments to provide a quantitative assessment of mass transport in the NASA rotating wall perfused vessel (RWPV) bioreactor to be flown on EDU#2. This effort consisted of a literature review of bioreactor mass transport studies, the extension of an existing scalar transport computer simulation to include production and utilization of the scalar, and the evaluation of experimental techniques for determining mass transport in these vessels. Since mass transport at the cell surface is determined primarily by the relative motion of the cell assemblage and the surrounding fluid, a detailed assessment of the relative motion was conducted. Results of the simulations of the motion of spheres in the RWPV under microgravity conditions are compared with flight data from EDU#1 flown on STS-70. The mass transport across the cell membrane depends upon the environment, the cell type, and the biological state of the cell. Results from a literature review of cell requirements of several scalars are presented. As a first approximation, a model with a uniform spatial distribution of utilization or production was developed and results from these simulations are presented. There were two candidate processes considered for the experimental mass transport evaluations. The first was to measure the dissolution rate of solid or gel beads. The second was to measure the induced fluorescence of beads as a stimulant (for example hydrogen peroxide) is infused into the vessel. Either technique would use video taped images of the process for recording the quantitative results. Results of preliminary tests of these techniques are discussed.

  1. Managing Transportation Infrastructure for Sustainable Development

    NARCIS (Netherlands)

    Akinyemi, Edward O.; Zuidgeest, Mark H.P.

    2002-01-01

    Major requirements for operationalization of the concept of sustainable development in urban transportation infrastructure operations management are presented. In addition, it is shown that the current approach to management is incompatible with the requirements for sustainable urban development. Co

  2. Optimal transport

    CERN Document Server

    Eckmann, B

    2008-01-01

    At the close of the 1980s, the independent contributions of Yann Brenier, Mike Cullen and John Mather launched a revolution in the venerable field of optimal transport founded by G Monge in the 18th century, which has made breathtaking forays into various other domains of mathematics ever since. The author presents a broad overview of this area.

  3. Transport system

    NARCIS (Netherlands)

    Drenth, K.F.

    1999-01-01

    The transport system comprises at least one road surface (2) and at least one vehicle (4) on wheels (6). The road surface (2) has a substantially bowl-shaped cross section and the vehicle (4) is designed so that the wheels (6) run directly on the road surface (2) while the road surface (2) acts as a

  4. Transport fuel

    DEFF Research Database (Denmark)

    Ronsse, Frederik; Jørgensen, Henning; Schüßler, Ingmar;

    2014-01-01

    Worldwide, the use of transport fuel derived from biomass increased four-fold between 2003 and 2012. Mainly based on food resources, these conventional biofuels did not achieve the expected emission savings and contributed to higher prices for food commod - ities, especially maize and oilseeds...

  5. Transport modeling

    Institute of Scientific and Technical Information of China (English)

    R.E. Waltz

    2007-01-01

    @@ There has been remarkable progress during the past decade in understanding and modeling turbulent transport in tokamaks. With some exceptions the progress is derived from the huge increases in computational power and the ability to simulate tokamak turbulence with ever more fundamental and physically realistic dynamical equations, e.g.

  6. Travel and transport

    DEFF Research Database (Denmark)

    Bill, Jan; Roesdahl, Else

    2007-01-01

    On the interrelationship between travel, transport and society; on land transport, sea and river transport, and on winter transport;  on the related technologies and their developments......On the interrelationship between travel, transport and society; on land transport, sea and river transport, and on winter transport;  on the related technologies and their developments...

  7. Software requirements

    CERN Document Server

    Wiegers, Karl E

    2003-01-01

    Without formal, verifiable software requirements-and an effective system for managing them-the programs that developers think they've agreed to build often will not be the same products their customers are expecting. In SOFTWARE REQUIREMENTS, Second Edition, requirements engineering authority Karl Wiegers amplifies the best practices presented in his original award-winning text?now a mainstay for anyone participating in the software development process. In this book, you'll discover effective techniques for managing the requirements engineering process all the way through the development cy

  8. Transportation Modelling for Regional Evacuations

    NARCIS (Netherlands)

    Pel, A.J.

    2011-01-01

    Regional evacuation planning is complex and timely. These planning studies can be assisted by a transportation model. In this thesis, we investigate the requirements for such a model, and develop, implement, and test a new model, called EVAQ, which meets these requirements. Two case studies show how

  9. Energy requirements

    NARCIS (Netherlands)

    Hulzebos, Christian V.; Sauer, Pieter J. J.

    2007-01-01

    The determination of the appropriate energy and nutritional requirements of a newborn infant requires a clear goal of the energy and other compounds to be administered, valid methods to measure energy balance and body composition, and knowledge of the neonatal metabolic capacities. Providing an appr

  10. Predicting water transport in multilayer coatings

    NARCIS (Netherlands)

    Baukh, V.; Huinink, H.P.; Adan, O.C.G.; Erich, S.J.F.; Ven, L.G.J. van der

    2012-01-01

    Water transport in polymer coatings is a key process in their failure. Mostly, such systems are composed of multiple layers to meet the required specifications. Understanding water transport requires a model that relates the kinetics with the properties of the layers. In this article a theoretical m

  11. Copper transport.

    Science.gov (United States)

    Linder, M C; Wooten, L; Cerveza, P; Cotton, S; Shulze, R; Lomeli, N

    1998-05-01

    In adult humans, the net absorption of dietary copper is approximately 1 mg/d. Dietary copper joins some 4-5 mg of endogenous copper flowing into the gastrointestinal tract through various digestive juices. Most of this copper returns to the circulation and to the tissues (including liver) that formed them. Much lower amounts of copper flow into and out of other major parts of the body (including heart, skeletal muscle, and brain). Newly absorbed copper is transported to body tissues in two phases, borne primarily by plasma protein carriers (albumin, transcuprein, and ceruloplasmin). In the first phase, copper goes from the intestine to the liver and kidney; in the second phase, copper usually goes from the liver (and perhaps also the kidney) to other organs. Ceruloplasmin plays a role in this second phase. Alternatively, liver copper can also exit via the bile, and in a form that is less easily reabsorbed. Copper is also present in and transported by other body fluids, including those bathing the brain and central nervous system and surrounding the fetus in the amniotic sac. Ceruloplasmin is present in these fluids and may also be involved in copper transport there. The concentrations of copper and ceruloplasmin in milk vary with lactational stage. Parallel changes occur in ceruloplasmin messenger RNA expression in the mammary gland (as determined in pigs). Copper in milk ceruloplasmin appears to be particularly available for absorption, at least in rats.

  12. Copper transport.

    Science.gov (United States)

    Linder, M C; Wooten, L; Cerveza, P; Cotton, S; Shulze, R; Lomeli, N

    1998-05-01

    In adult humans, the net absorption of dietary copper is approximately 1 mg/d. Dietary copper joins some 4-5 mg of endogenous copper flowing into the gastrointestinal tract through various digestive juices. Most of this copper returns to the circulation and to the tissues (including liver) that formed them. Much lower amounts of copper flow into and out of other major parts of the body (including heart, skeletal muscle, and brain). Newly absorbed copper is transported to body tissues in two phases, borne primarily by plasma protein carriers (albumin, transcuprein, and ceruloplasmin). In the first phase, copper goes from the intestine to the liver and kidney; in the second phase, copper usually goes from the liver (and perhaps also the kidney) to other organs. Ceruloplasmin plays a role in this second phase. Alternatively, liver copper can also exit via the bile, and in a form that is less easily reabsorbed. Copper is also present in and transported by other body fluids, including those bathing the brain and central nervous system and surrounding the fetus in the amniotic sac. Ceruloplasmin is present in these fluids and may also be involved in copper transport there. The concentrations of copper and ceruloplasmin in milk vary with lactational stage. Parallel changes occur in ceruloplasmin messenger RNA expression in the mammary gland (as determined in pigs). Copper in milk ceruloplasmin appears to be particularly available for absorption, at least in rats. PMID:9587137

  13. The genetics of axonal transport and axonal transport disorders.

    Directory of Open Access Journals (Sweden)

    Jason E Duncan

    2006-09-01

    Full Text Available Neurons are specialized cells with a complex architecture that includes elaborate dendritic branches and a long, narrow axon that extends from the cell body to the synaptic terminal. The organized transport of essential biological materials throughout the neuron is required to support its growth, function, and viability. In this review, we focus on insights that have emerged from the genetic analysis of long-distance axonal transport between the cell body and the synaptic terminal. We also discuss recent genetic evidence that supports the hypothesis that disruptions in axonal transport may cause or dramatically contribute to neurodegenerative diseases.

  14. Transportation 2000. Spent fuel transportation trends in the new millenium

    International Nuclear Information System (INIS)

    The paper will provide a comparison of foreign research reactor spent fuel transportation today verses the assumptions used by the Department of Energy in the Environmental Impact Statement. In addition, it will suggest changes that are likely to occur in transportation logistics through the remainder of the U.S. spent fuel returns program. Cask availability, certification status, shipment strategy, cost issues, and public acceptance are among the topical areas that will be examined. Transportation requirements will be assessed in light of current participation in the returns program and the tendency for shipment plans to shift toward spent fuel return toward the end of the 13 year period of eligibility. (author)

  15. 49 CFR 383.111 - Required knowledge.

    Science.gov (United States)

    2010-10-01

    ... regulations. Driver-related elements of the regulations contained in 49 CFR parts 382, 391, 392, 393, 395, 396... 49 Transportation 5 2010-10-01 2010-10-01 false Required knowledge. 383.111 Section 383.111... STANDARDS; REQUIREMENTS AND PENALTIES Required Knowledge and Skills § 383.111 Required knowledge....

  16. 49 CFR 383.113 - Required skills.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 5 2010-10-01 2010-10-01 false Required skills. 383.113 Section 383.113... STANDARDS; REQUIREMENTS AND PENALTIES Required Knowledge and Skills § 383.113 Required skills. (a) Basic vehicle control skills. All applicants for a CDL must possess and demonstrate basic motor vehicle...

  17. Intramanchette transport (IMT): managing the making of the spermatid head, centrosome, and tail.

    Science.gov (United States)

    Kierszenbaum, Abraham L

    2002-09-01

    Intramanchette transport (IMT) and intraflagellar transport (IFT) share similar molecular components: a raft protein complex transporting cargo proteins mobilized along microtubules by molecular motors. IFT, initially discovered in flagella of Chlamydomonas, has been also observed in cilia of the worm Caenorhabditis elegans and in mouse ciliated and flagellated cells. IFT has been defined as the mechanism by which protein raft components (also called IFT particles) are displaced between the flagellum and the plasma membrane in the anterograde direction by kinesin-II and in the retrograde direction by cytoplasmic dynein 1b. Mutation of the gene Tg737, encoding one of the components of the raft protein complex, designated Polaris in the mouse and IFT88 in both Chlamydomonas and mouse, results in defective ciliogenesis and flagellar development as well as asymmetry in left-right axis determination. Polaris/IFT88 is detected in the manchette of mouse and rat spermatids. Indications of an IMT mechanism originated from the finding that two proteins associated with the manchette (Sak57/K5 and TBP-1, the latter a component of the 26S proteasome) repositioned to the centrosome and sperm tail once the manchette disassembled. IMT has the features of the IFT machinery but, in addition, facilitates nucleocytoplasmic exchange activities during spermiogenesis. An example is Ran, a small GTPase present in the nucleus and cytoplasm of round spermatids and in the manchette of elongating spermatids. Upon disassembly of the manchette, Ran GTPase is found in the centrosome region of elongating spermatids. Because defective molecular motors and raft proteins result in defective flagella, cilia, and cilia-containing photoreceptor cells in the retina, IMT and IFT are emerging as essential mechanisms for managing critical aspects of sperm development. Details of specific role of Ran GTPase in nucleocytoplasmic transport and its relocation from the manchette to the centrosome to the sperm

  18. Functional Analysis of Arabidopsis Sucrose Transporters

    Energy Technology Data Exchange (ETDEWEB)

    John M. Ward

    2009-03-31

    Sucrose is the main photosynthetic product that is transported in the vasculature of plants. The long-distance transport of carbohydrates is required to support the growth and development of net-importing (sink) tissues such as fruit, seeds and roots. This project is focused on understanding the transport mechanism sucrose transporters (SUTs). These are proton-coupled sucrose uptake transporters (membrane proteins) that are required for transport of sucrose in the vasculature and uptake into sink tissues. The accomplishments of this project included: 1) the first analysis of substrate specificity for any SUT. This was accomplished using electrophysiology to analyze AtSUC2, a sucrose transporter from companion cells in Arabidopsis. 2) the first analysis of the transport activity for a monocot SUT. The transport kinetics and substrate specificity of HvSUT1 from barley were studied. 3) the first analysis of a sucrose transporter from sugarcane. and 4) the first analysis of transport activity of a sugar alcohol transporter homolog from plants, AtPLT5. During this period four primary research papers, funded directly by the project, were published in refereed journals. The characterization of several sucrose transporters was essential for the current effort in the analysis of structure/function for this gene family. In particular, the demonstration of strong differences in substrate specificity between type I and II SUTs was important to identify targets for site-directed mutagenesis.

  19. Transportation System Concept of Operations

    Energy Technology Data Exchange (ETDEWEB)

    N. Slater-Thompson

    2006-08-16

    The Nuclear Waste Policy Act of 1982 (NWPA), as amended, authorized the DOE to develop and manage a Federal system for the disposal of SNF and HLW. OCRWM was created to manage acceptance and disposal of SNF and HLW in a manner that protects public health, safety, and the environment; enhances national and energy security; and merits public confidence. This responsibility includes managing the transportation of SNF and HLW from origin sites to the Repository for disposal. The Transportation System Concept of Operations is the core high-level OCRWM document written to describe the Transportation System integrated design and present the vision, mission, and goals for Transportation System operations. By defining the functions, processes, and critical interfaces of this system early in the system development phase, programmatic risks are minimized, system costs are contained, and system operations are better managed, safer, and more secure. This document also facilitates discussions and understanding among parties responsible for the design, development, and operation of the Transportation System. Such understanding is important for the timely development of system requirements and identification of system interfaces. Information provided in the Transportation System Concept of Operations includes: the functions and key components of the Transportation System; system component interactions; flows of information within the system; the general operating sequences; and the internal and external factors affecting transportation operations. The Transportation System Concept of Operations reflects OCRWM's overall waste management system policies and mission objectives, and as such provides a description of the preferred state of system operation. The description of general Transportation System operating functions in the Transportation System Concept of Operations is the first step in the OCRWM systems engineering process, establishing the starting point for the lower

  20. Symmetric solid target transport system

    OpenAIRE

    Tomov, D.; Lawrence, L; Gaehle, G.

    2015-01-01

    Introduction The expansion of our PET isotope production with a new TR-19 cyclotron necessitated a suitable solid target transport system. None of the known existing and proposed solid target transport systems (STTS) was able to meet the technical and budget requirements of the MIR cyclotron facility [5]. A unique carrier design allowed us to develop a fully automated 50.8 mm inner diameter pneumatic tube STTS with an in-hot-cell compact form factor receiving station. The cyclotron or v...

  1. Transporter Classification Database (TCDB)

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Transporter Classification Database details a comprehensive classification system for membrane transport proteins known as the Transporter Classification (TC)...

  2. Very long-chain fatty acid-containing lipids rather than sphingolipids per se are required for raft association and stable surface transport of newly synthesized plasma membrane ATPase in yeast.

    Science.gov (United States)

    Gaigg, Barbara; Toulmay, Alexandre; Schneiter, Roger

    2006-11-10

    The proton-pumping H+-ATPase, Pma1p, is an abundant and very long lived polytopic protein of the yeast plasma membrane. Pma1p constitutes a major cargo of the secretory pathway and thus serves as a model to study plasma membrane biogenesis. Pma1p associates with detergent-resistant membrane domains (lipid "rafts") already in the ER, and a lack of raft association correlates with mistargeting of the protein to the vacuole, where it is degraded. We are analyzing the role of specific lipids in membrane domain formation and have previously shown that surface transport of Pma1p is independent of newly synthesized sterols but that sphingolipids with C26 very long chain fatty acid are crucial for raft association and surface transport of Pma1p (Gaigg, B., Timischl, B., Corbino, L., and Schneiter, R. (2005) J. Biol. Chem. 280, 22515-22522). We now describe a more detailed analysis of the function that sphingolipids play in this process. Using a yeast strain in which the essential function of sphingolipids is substituted by glycerophospholipids containing C26 very long chain fatty acids, we find that sphingolipids per se are dispensable for raft association and surface delivery of Pma1p but that the C26 fatty acid is crucial. We thus conclude that the essential function of sphingolipids for membrane domain formation and stable surface delivery of Pma1p is provided by the C26 fatty acid that forms part of the yeast ceramide.

  3. COMPARATIVE ANALYSIS OF TRANSPORT AIRCRAFT, BACKROUND FOR SHORT/ MEDIUM COURIER TRANSPORT AIRCRAFT PROCUREMENT

    Directory of Open Access Journals (Sweden)

    Matei POPA

    2010-03-01

    Full Text Available In accordance with Air Force requirements, the comparative analysis of short/medium transport aircraft comes to sustain procurement decision of short/medium transport aircraft. This paper presents, in short, the principles and the results of the comparative analysis for short/medium military transport aircraft.

  4. Mobile Transporter

    Science.gov (United States)

    2001-01-01

    The Space Shuttle Atlantis, STS-110 mission, deployed this railcar, called the Mobile Transporter, and an initial 43-foot section of track, the S0 (S-zero) truss, preparing the International Space Station (ISS) for future spacewalks. The first railroad in space, the Mobile Transporter will allow the Station's robotic arm to travel up and down the finished truss for future assembly and maintenance. The 27,000-pound S0 truss is the first of 9 segments that will make up the Station's external framework that will eventually stretch 356 feet (109 meters), or approximately the length of a football field. The completed truss structure will hold solar arrays and radiators to provide power and cooling for additional international research laboratories from Japan and Europe that will be attached to the Station. The Space Shuttle Orbiter Atlantis, STS-110 mission, was launched April 8, 2002 and returned to Earth April 19, 2002. STS-110's Extravehicular Activity (EVA) marked the first use of the Station's robotic arm to maneuver spacewalkers around the Station.

  5. Proton Transport

    Science.gov (United States)

    Pohorille, Andrew; DeVincenzi, Donald L. (Technical Monitor)

    2001-01-01

    The transport of protons across membranes is an essential process for both bioenergetics of modern cells and the origins of cellular life. All living systems make use of proton gradients across cell walls to convert environmental energy into a high-energy chemical compound, adenosine triphosphate (ATP), synthesized from adenosine diphosphate. ATP, in turn, is used as a source of energy to drive many cellular reactions. The ubiquity of this process in biology suggests that even the earliest cellular systems were relying on proton gradient for harvesting environmental energy needed to support their survival and growth. In contemporary cells, proton transfer is assisted by large, complex proteins embedded in membranes. The issue addressed in this Study was: how the same process can be accomplished with the aid of similar but much simpler molecules that could have existed in the protobiological milieu? The model system used in the study contained a bilayer membrane made of phospholipid, dimyristoylphosphatidylcholine (DMPC) which is a good model of the biological membranes forming cellular boundaries. Both sides of the bilayer were surrounded by water which simulated the environment inside and outside the cell. Embedded in the membrane was a fragment of the Influenza-A M$_2$ protein and enough sodium counterions to maintain system neutrality. This protein has been shown to exhibit remarkably high rates of proton transport and, therefore, is an excellent model to study the formation of proton gradients across membranes. The Influenza M$_2$ protein is 97 amino acids in length, but a fragment 25 amino acids long. which contains a transmembrane domain of 19 amino acids flanked by three amino acids on each side. is sufficient to transport protons. Four identical protein fragments, each folded into a helix, aggregate to form small channels spanning the membrane. Protons are conducted through a narrow pore in the middle of the channel in response to applied voltage. This

  6. Visually Exploring Transportation Schedules.

    Science.gov (United States)

    Palomo, Cesar; Guo, Zhan; Silva, Cláudio T; Freire, Juliana

    2016-01-01

    Public transportation schedules are designed by agencies to optimize service quality under multiple constraints. However, real service usually deviates from the plan. Therefore, transportation analysts need to identify, compare and explain both eventual and systemic performance issues that must be addressed so that better timetables can be created. The purely statistical tools commonly used by analysts pose many difficulties due to the large number of attributes at trip- and station-level for planned and real service. Also challenging is the need for models at multiple scales to search for patterns at different times and stations, since analysts do not know exactly where or when relevant patterns might emerge and need to compute statistical summaries for multiple attributes at different granularities. To aid in this analysis, we worked in close collaboration with a transportation expert to design TR-EX, a visual exploration tool developed to identify, inspect and compare spatio-temporal patterns for planned and real transportation service. TR-EX combines two new visual encodings inspired by Marey's Train Schedule: Trips Explorer for trip-level analysis of frequency, deviation and speed; and Stops Explorer for station-level study of delay, wait time, reliability and performance deficiencies such as bunching. To tackle overplotting and to provide a robust representation for a large numbers of trips and stops at multiple scales, the system supports variable kernel bandwidths to achieve the level of detail required by users for different tasks. We justify our design decisions based on specific analysis needs of transportation analysts. We provide anecdotal evidence of the efficacy of TR-EX through a series of case studies that explore NYC subway service, which illustrate how TR-EX can be used to confirm hypotheses and derive new insights through visual exploration.

  7. 49 CFR 192.513 - Test requirements for plastic pipelines.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 3 2010-10-01 2010-10-01 false Test requirements for plastic pipelines. 192.513 Section 192.513 Transportation Other Regulations Relating to Transportation (Continued) PIPELINE AND... Test requirements for plastic pipelines. (a) Each segment of a plastic pipeline must be tested...

  8. 49 CFR 577.9 - Conformity to statutory requirements.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 7 2010-10-01 2010-10-01 false Conformity to statutory requirements. 577.9 Section 577.9 Transportation Other Regulations Relating to Transportation (Continued) NATIONAL HIGHWAY... NOTIFICATION § 577.9 Conformity to statutory requirements. A notification that does not conform to...

  9. Tourism and the Problems of Developing Transport Infrastructure in Ukraine

    OpenAIRE

    Natalija Tkachenko

    2011-01-01

    The main problems, associated with the development of a transport network in Ukraine and the need for complex evaluation of the interests of the states, seeking international cooperation in creating transport corridors for transporting tourists and determining the position of Ukraine in this cooperation, are considered. The main requirements to design of highways and transport infrastructure are defined. Article in Russian

  10. 46 CFR 148.03-7 - During transport.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false During transport. 148.03-7 Section 148.03-7 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) DANGEROUS CARGOES CARRIAGE OF SOLID HAZARDOUS MATERIALS IN BULK Minimum Transportation Requirements § 148.03-7 During transport. During the transport of...

  11. 41 CFR 109-40.103-3 - International transportation.

    Science.gov (United States)

    2010-07-01

    ..., TRANSPORTATION, AND MOTOR VEHICLES 40-TRANSPORTATION AND TRAFFIC MANAGEMENT 40.1-General Provision § 109-40.103-3 International transportation. See 4 CFR 52.2 for a certificate required in nonuse of U.S. flag vessels or U.S... transportation. 109-40.103-3 Section 109-40.103-3 Public Contracts and Property Management Federal...

  12. 41 CFR 50-204.75 - Transportation safety.

    Science.gov (United States)

    2010-07-01

    ... Transportation Safety § 50-204.75 Transportation safety. Any requirements of the U.S. Department of Transportation under 49 CFR Parts 171-179 and Parts 390-397 and 14 CFR Part 103 shall be applied to... 41 Public Contracts and Property Management 1 2010-07-01 2010-07-01 true Transportation safety....

  13. 29 CFR 780.909 - “Transportation.”

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 3 2010-07-01 2010-07-01 false âTransportation.â 780.909 Section 780.909 Labor Regulations... Vegetable Harvest Transportation; Exemption From Overtime Pay Requirements Under Section 13(b)(16) Exempt Operations on Fruits Or Vegetables § 780.909 “Transportation.” “Transportation,” as used in section...

  14. The water footprint of biofuel-based transport

    NARCIS (Netherlands)

    Gerbens-Leenes, P.W.; Hoekstra, A.Y.

    2011-01-01

    The EU target to replace 10 percent of transport fuels by renewables by 2020 requires additional water. This study calculates water footprints (WFs) of transport modes using first generation bio-ethanol, biodiesel or bio-electricity and of European transport if 10 percent of transport fuels is bio-e

  15. State and local government regulation of nuclear waste transportation

    International Nuclear Information System (INIS)

    The Hazardous Materials Transportation Act (HMTA), authorizes the U.S. Department of Transportation (DOT) to promulgate rules governing the safe transportation in commerce of hazardous materials, including nuclear waste. The HMTA further provides that any state or local government requirement is preempted and thus invalid if it is inconsistent with a DOT requirement issued pursuant to the HMTA

  16. AIR TRANSPORTS – COMPONENT OF INTERNATIONAL TRANSPORTS

    Directory of Open Access Journals (Sweden)

    Mihaela Loredana LĂPĂDUŞI

    2009-12-01

    Full Text Available Air transports activity has known an important development caused by the economic increase, by Romania’s involvement in the international products trade, in international tourism. They are completed by the specific characteristics of air transports, which, together with the characteristics of the other ways of transport, has certain transport objectives with a higher and higher significance. Air traffic has a national commercial value and thus practices have been established in approaching national policies regarding: internal traffic protection through national air transporters, granting access to foreign transporters to national traffic in international transports.

  17. Engaging Undergraduate Students in Transportation Studies through Simulating Transportation for Realistic Engineering Education and Training (STREET)

    OpenAIRE

    Chen-Fu Liao; David Levinson; Henry Liu

    2008-01-01

    The practice of transportation engineering and planning has evolved substantially over the past several decades. A new paradigm for transportation engineering education is required to better engage students and deliver knowledge. Simulation tools have been used by transportation professionals to evaluate and analyze the potential impact of design or control strategy changes. Conveying complex transportation concepts can be effectively achieved by exploring them through simulation. Simulation ...

  18. Transcriptional profiling of C. elegans DAF-19 uncovers a ciliary base-associated protein and a CDK/CCRK/LF2p-related kinase required for intraflagellar transport

    OpenAIRE

    Phirke, Prasad; Efimenko, Evgeni; Mohan, Swetha; Burghoorn, Jan; Crona, Filip; Bakhoum, Mathieu W.; Trieb, Maria; Schuske, Kim; Erik M. Jorgensen; Piasecki, Brian P.; Leroux, Michel R.; Swoboda, Peter

    2011-01-01

    Cilia are ubiquitous cell surface projections that mediate various sensory- and motility-based processes and are implicated in a growing number of multi-organ genetic disorders termed ciliopathies. To identify new components required for cilium biogenesis and function, we sought to further define and validate the transcriptional targets of DAF-19, the ciliogenic C. elegans RFX transcription factor. Transcriptional profiling of daf-19 mutants (which do not form cilia) and wild-type animals was...

  19. Next Generation Reliable Transport Networks

    DEFF Research Database (Denmark)

    Zhang, Jiang

    -Transport Profile (MPLS-TP) ring networks are addressed. Through the proposed protection structure and protection switching schemes, the recovery mechanism is enhanced in terms of recovery label consumption, operation simplicity and fine traffic engineering granularity. Furthermore, the extensions for existing...... and proposed protection schemes on the interconnected-ring structure are presented, which not only fulfill the requirements suggested by ITU and IETF organizations, but also increase the scalability and applicability of the transport ring networks. Secondly, avionic transport networks are investigated, aiming...... at enabling the full exploitation of key optical networking technologies in future aircrafts. A three-layered optical transport networks over a ring topology is suggested, as it can provide full reconfiguration flexibility and support a wide range of avionic applications. According to different levels...

  20. 48 CFR 1206.501 - Requirement.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Requirement. 1206.501 Section 1206.501 Federal Acquisition Regulations System DEPARTMENT OF TRANSPORTATION ACQUISITION PLANNING COMPETITION REQUIREMENTS Competition Advocates 1206.501 Requirement. The DOT Senior Competition Advocate...

  1. 49 CFR 176.118 - Electrical requirement.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 2 2010-10-01 2010-10-01 false Electrical requirement. 176.118 Section 176.118... Requirements for Class 1 (Explosive) Materials Stowage § 176.118 Electrical requirement. (a) Electrical..., opening of switches or circuit breakers, or disconnection from bus bars. The means, or access to the...

  2. Ceramic oxygen transport membrane array reactor and reforming method

    Energy Technology Data Exchange (ETDEWEB)

    Kelly, Sean M.; Christie, Gervase Maxwell; Rosen, Lee J.; Robinson, Charles; Wilson, Jamie R.; Gonzalez, Javier E.; Doraswami, Uttam R.

    2016-09-27

    A commercially viable modular ceramic oxygen transport membrane reforming reactor for producing a synthesis gas that improves the thermal coupling of reactively-driven oxygen transport membrane tubes and catalyst reforming tubes required to efficiently and effectively produce synthesis gas.

  3. Multi-Agent Systems for Transportation Planning and Coordination

    NARCIS (Netherlands)

    J.M. Moonen (Hans)

    2009-01-01

    textabstractMany transportation problems are in fact coordination problems: problems that require communication, coordination and negotiation to be optimally solved. However, most software systems targeted at transportation have never approached it this way, and have instead concentrated on centrali

  4. Chemical transport reactions

    CERN Document Server

    Schäfer, Harald

    2013-01-01

    Chemical Transport Reactions focuses on the processes and reactions involved in the transport of solid or liquid substances to form vapor phase reaction products. The publication first offers information on experimental and theoretical principles and the transport of solid substances and its special applications. Discussions focus on calculation of the transport effect of heterogeneous equilibria for a gas motion between equilibrium spaces; transport effect and the thermodynamic quantities of the transport reaction; separation and purification of substances by means of material transport; and

  5. ELECTROCHEMICAL POWER FOR TRANSPORTATION

    Energy Technology Data Exchange (ETDEWEB)

    Cairns, Elton J.; Hietbrink, Earl H.

    1981-01-01

    This section includes some historical background of the rise and fall and subsequent rebirth of the electric vehicle; and a brief discussion of current transportation needs, and environmental and energy utilization issues that resulted in the renewed interest in applying electrochemical energy conversion technology to electric vehicle applications. Although energy utilization has evolved to be the most significant and important issue, the environmental issue will be discussed first in this section only because of its chronological occurrence. The next part of the chapter is a review of passenger and commercial electric vehicle technology with emphasis on vehicle design and demonstrated performance of vehicles with candidate power sources being developed. This is followed by a discussion of electrochemical power source requirements associated with future electric vehicles that can play a role in meeting modern transportation needs. The last part of the chapter includes first a discussion of how to identify candidate electrochemical systems that might be of interest in meeting electric vehicle power source requirements. This is then followed by a review of the current technological status of these systems and a discussion of the most significant problems that must be resolved before each candidate system can be a viable power source.

  6. Effective Transport Properties

    Science.gov (United States)

    Mauri, Roberto

    In this chapter we study a particular case of multiphase systems, namely two-phase materials in which one of the phases is randomly dispersed in the other, so that the composite can be viewed on a macroscale as an effective continuum, with well defined properties. In general, the theoretical determination of the parameter for an effective medium requires, as a rule, the solution of a corresponding transport problem at the microscale, which takes into account the morphology of the system and its evolution. As the mathematical problem is well-posed on a microscale, this can be accomplished using, for example, the multiple scale approach shown in Chap. 11 ; however, the task requires massive computations and is therefore difficult to implement from the practical standpoint. Here, instead, we focus on a deterministic approach to the problem, where the geometry and spatial configuration of the particles comprising the included phase are given and the solution to the microscale problem is therefore sought analytically. As examples, we study the effective thermal conductivity of solid reinforced materials (Sect. 10.1), the effective viscosity of non-colloidal suspensions (Sect. 10.2), the effective permeability of porous materials (10.3) and the effective self- and gradient diffusivities of colloidal suspensions (Sect. 10.4). Then, in Sect. 10.5, an alternative dynamic definition of the transport coefficients is considered, which can also serve as a basis to determine the effective properties of complex systems.

  7. Interface area transport of monodispersed spherical particulates

    Energy Technology Data Exchange (ETDEWEB)

    Chang, Chong H. [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

    2016-08-05

    We present an interface area transport model required in tracking of mass, momentum, and energy exchange between dispersed and background materials. The basic transport equation has been rigorously derived from the volume fraction evolution equation. Interface area changes due to mass transport and local compression/expansion are included. The model is then simplified for the case in which the dispersed phase is composed of spheres of locally uniform size. A procedure for calculating advective flux with interface reconstruction has been suggested.

  8. Metabolismo miocárdico após cardioplegia sangüínea hipotérmica retrógrada contínua com indução anterógrada normotérmica Myocardial metabolism after hypothermic retrograde continuous blood cardioplegia with anterograde warm cardioplegic induction

    Directory of Open Access Journals (Sweden)

    Claudio G. Sobrosa

    2005-12-01

    continuous blood cardioplegia with antegrade warm cardioplegic induction. METHOD: A metabolic analysis of hypothermic retrograde continuous blood cardioplegia with antegrade warm cardioplegic induction was performed in a prospective study of 15 patients scheduled for elective coronary artery bypass grafting. Arterial and coronary sinus blood samples were simultaneously taken: before establishing cardiopulmonary bypass, after anterograde warm cardioplegic induction, when the aortic clamp was removed and 10, 30 and 60 minutes after reperfusion to analyze the oxygen content and lactate concentration. Four transmural left ventricular biopsy samples were obtained: before aortic clamping, immediately after the initial cardioplegia bolus, immediately before aortic declamping and 30 minutes after reperfusion to analyze the levels of ATP, ADP, AMP and lactate in the myocardium. The CK-MB isoenzyme was analysed in venous blood samples. RESULTS: There were no mortalities in the group. Inotropic support was not necessary in any patients and no peri- or post-operative myocardial infarction was detected. There was a decrease in the arterial-venous extraction of oxygen and lactate in the heart during reperfusion, a partial recovery occurred at 60 minutes of reperfusion. The levels of ATP and the other nucleotides in the myocardium were maintained during aortic clamping, but these levels decreased during the first 30 minutes of reperfusion. The lactate accumulated in the heart muscle during aortic clamping with a decrease occurring during reperfusion. CONCLUSIONS: From a metabolic point of view the method could not avoid an anaerobic metabolism during cross-clamping and only after 60 minutes of reperfusion there was a satisfactory metabolic recovery. These alterations are probably a reflection of cellular ischemic injury that occurs during cross-clamping and they seem to be of transitory effect. A better myocardium protection was observed with the addiction of anterograde warm induction

  9. 不同剂量右美托咪定对妇科手术患者异丙酚顺行性遗忘作用的影响%Effects of different doses of dexmedetomidine on anterograde amnesia of propofol in patients undergoing gynecological surgery

    Institute of Scientific and Technical Information of China (English)

    张伟; 茅晓玉; 张卫

    2014-01-01

    目的 评价不同剂量右美托咪定对妇科手术患者异丙酚顺行性遗忘作用的影响.方法 择期行腹腔镜下全子宫切除术患者60例,年龄20~50岁,体重指数18 ~ 25 kg/m2,ASA分级Ⅰ或Ⅱ级,采用随机数字表法,将其分为3组(n=20):异丙酚组(C组)、低剂量右美托咪定复合异丙酚组(D1组)和高剂量右美托咪定复合异丙酚组(D2组).D1组和D2组分别经15 min静脉输注右美托咪定0.2、0.4 μg/kg.随后3组靶控输注异丙酚,初始血浆靶浓度(Cp)为0.5 μg/ml,依次递增至0.9、1.3、1.7、2.1 μg/ml,每一浓度维持3 min.分别于入室后(基础水平)、右美托咪定输注完毕、异丙酚Cp达0.5、0.9、1.3、1.7、2.1 μg/ml维持3 min时进行遗忘测试和警觉/镇静评分(OAA/S评分),计算遗忘率达95%时异丙酚Cp和BIS值,并记录该期间低血压、心动过缓、呼吸抑制和上呼吸道梗阻的发生情况.结果 与C组比较,D1组和D2组遗忘率升高,遗忘率达95%时异丙酚Cp和呼吸抑制发生率降低,BIS值升高(P<0.05),其他不良事件发生率差异无统计学意义(P>0.05);与D1组比较,D2组遗忘率升高,遗忘率达95%时异丙酚Cp降低,BIS值升高(P<0.05),D1组和D2组不良事件发生率差异无统计学意义(P>0.05).结论 右美托咪定增强妇科手术患者异丙酚顺行性遗忘作用的适宜剂量为0.4 μg/kg.%Objective To evaluate the effects of different doses of dexmedetomidine on the anterograde amnesia of propofol in the patients undergoing gynecological surgery.Methods Sixty patients,aged 20-50 yr,with body mass index of 18-25 kg/m2,of ASA physical status Ⅰ or Ⅱ,scheduled for elective laparoscopic total hysterectomy,were randomly divided into 3 groups (n =20 each) using a random number table:propofol group (group C),low-dose dexmedetomidine combined with propofol group (group D1) and high-dose dexmedetomidine combined with propofol group (group D2).Dexmedetomidine 0.2 and 0.4 mg/kg were

  10. 咪达唑仑和丙泊酚靶控输注用于清醒镇静患者遗忘作用的比较分析%Comparative analysis of target-controlled infusion of midazolam and propofol on anterograde amnesia in sober calm patient

    Institute of Scientific and Technical Information of China (English)

    周冠同; 黄梅英; 陈新妹; 温清娴; 李金波; 吴开华

    2012-01-01

    Objective To compare the difference of target-controlled infusion of midazolam and propofol on anterograde amnesia in sober calm patient.Methods One hundred and twenty patients underwent the epidural anesthesia for the abdomen or legs operation were randomly divided into observation group and control group,every group for 60 cases.Patient in control group were given target-controlled infusion of propofol,patient in observation group were given target-controlled infusion of midazolam.Calmness depth were assessed by OAA/S mark criterion.The changes of SBP,DBP,HR,RR,SpO2 in the different time were recorded.Results The SBP,DBP,HR,RR,SpO2 in the observation group were not changed,HR in the control group was increased after intravenous injection for 1-5 min(P < 0.05),return to onrmal after ten to twenty minutes; and the SBP,DBP,RR were not changed in the other time.The SpO2 was 95%-100%.The OAA/S mark was reduced from 4 to 2,calmness depth was gradually deepen,and the forgetting rate was gradually rising.Patients in 2-3 mark was 93.3% and 65.0%,the complete forgetting rate was 91.7% and 25.0%,the observation group was better than the control group (P <0.05).Conclusions Both of midazolam and propofol have anterograde amnesia effect,and the propofol presented the effects in deeper sedation,midazolam has rerograde amnesia effect in shallow calm.%目的 比较咪达唑仑和丙泊酚靶控输注用于清醒镇静患者遗忘作用的差异.方法 选择120例硬膜外麻醉下择期行腹部及下肢手术的患者,并随机分为观察组和对照组,每组60例,观察组靶控输注咪达唑仑,对照组靶控输注丙泊酚.用OAA/S评分标准评定镇静深度,并记录.记录用药后不同时间点的收缩压(SBP)、舒张压(DBP)、呼吸(HR)、心率(RR)、脉搏氧饱和度(SpO2)的变化.结果 观察组SBP、DBP、HR 、RR 、Sp02平稳,对照组静脉注射后l~5 min HR明显加快(P<0.05),10~20 min恢复;其余观察时

  11. Radioactive material accidents in the transport

    International Nuclear Information System (INIS)

    Transport is an important part of the worldwide nuclear industry and the safety record for nuclear transport across the world is excellent. The increase in the use of radioactive materials in our country requires that these materials be moved from production sites to the end user. Despite the number of packages transported, the number of incidents and accidents in which they are involved is low. In Brazil, do not be records of victims of the radiation as a result of the transport of radioactive materials and either due to the accidents happened during the transports. The absence of victims of the radiation as result of accidents during the transports is a highly significant fact, mainly to consider that annually approximately two hundred a thousand packages containing radioactive material are consigned for transport throughout the country, of which eighty a thousand are for a medical use. This is due to well-founded regulations developed by governmental and intergovernmental organizations and to the professionalism of those in the industry. In this paper, an overview is presented of the activities related to the transport of radioactive material in the state of Sao Paulo. The applicable legislation, the responsibilities and tasks of the competent authorities are discussed. The categories of radioactive materials transported and the packaging requirements for the safe transport of these radioactive materials are also described. It also presents the packages amounts of carried and the accidents occurred during the transport of radioactive materials, in the last five years. The main occurred events are argued, demonstrating that the demanded requirements of security for any transport of radioactive material are enough to guarantee the necessary control of ionizing radiation expositions to transport workers, members of general public and the environment. (author)

  12. Plant Transporter Identification

    DEFF Research Database (Denmark)

    Larsen, Bo

    Membrane transport proteins (transporters) play a critical role for numerous biological processes, by controlling the movements of ions and molecules in and out of cells. In plants, transporters thus function as gatekeepers between the plant and its surrounding environment and between organs......, tissues, cells and intracellular compartments. Since plants are highly compartmentalized organisms with complex transportation infrastructures, they consequently have many transporters. However, the vast majority of predicted transporters have not yet been experimentally verified to have transport...... activity. This project contains a review of the implemented methods, which have led to plant transporter identification, and present our progress on creating a high-throughput functional genomics transporter identification platform....

  13. 49 CFR 223.15 - Requirements for existing passenger cars.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 4 2010-10-01 2010-10-01 false Requirements for existing passenger cars. 223.15... ADMINISTRATION, DEPARTMENT OF TRANSPORTATION SAFETY GLAZING STANDARDS-LOCOMOTIVES, PASSENGER CARS AND CABOOSES Specific Requirements § 223.15 Requirements for existing passenger cars. (a) Passenger cars built...

  14. 49 CFR 639.11 - Lease qualification requirements.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 7 2010-10-01 2010-10-01 false Lease qualification requirements. 639.11 Section... ADMINISTRATION, DEPARTMENT OF TRANSPORTATION CAPITAL LEASES Requirements § 639.11 Lease qualification requirements. (a) A lease may qualify for capital assistance if it meets the following criteria: (1)...

  15. Towards low carbon transport in Europe

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2012-05-15

    The ambitious target of 60% reduction in greenhouse gas emissions by 2050 requires transformation of the transport system in Europe. Decarbonising transport calls for the use of less and cleaner energy, and more efficient use of modern transport infrastructure and services. These strategic targets in EU policy are supported by substantial investment in research to develop innovative and sustainable solutions in efficient use of transport infrastructure and services, more energy-efficient transport, and use of alternative fuels and propulsion systems. This Policy Brochure, which is produced by the Transport Research and Innovation Portal (TRIP), highlights the contribution of research and innovation in meeting the EU targets on CO2 emission reduction in the transport system.

  16. Transport regulations for radioactive materials in Japan

    International Nuclear Information System (INIS)

    The IAEA Regulations for the Safe Transport of Radioactive Materials are taken as a basis for our national regulations to secure the safe transport of radioactive materials in Japan. The requirements for packaging and packages that are used to transport radioactive materials are based on the IAEA Regulations. Furthermore, in addition to the IAEA Regulations, the relative international regulations such as the IMDG code of IMO etc. are reviewed by our national authorities and applied to the conveyance such as the transport vehicle/vessel etc. that is used for the shipment. The transport system such as a transport caravan in Japan is intended to provide even greater safety during transport. The future activities to be discussed in the IAEA are how to adopt the IAEA Regulations to Member States as soon as possible and to evaluate the safety margin of the IAEA Regulations to the assumed accidents. (author)

  17. Burning water: the water footprint of biofuel-based transport

    OpenAIRE

    Gerbens-Leenes, P.W.; Hoekstra, A.Y.

    2010-01-01

    The trend towards substitution of conventional transport fuels by biofuels requires additional water. The EU aims to replace 10 percent of total transport fuels by biofuels by 2020. This study calculates the water footprint (WF) of different transport modes using bio-ethanol, biodiesel or bio-electricity and of European transport if 10 percent of transport fuels is replaced by bio-ethanol. We compare results for Europe with similar goals for other regions (Africa, Asia, Latin America, the for...

  18. Assessment of LANL transportation policies and procedures

    International Nuclear Information System (INIS)

    In order to determine whether activities related to the transportation of waste at Los Alamos National Laboratory (LANL) were being conducted in accordance with DOE policy, requirements stated in applicable DOE Orders were reviewed and compared with LANL policies and procedures described in the Administrative Requirements and the On-Site Transportation Manual. The following DOE Orders were determined to pertain to waste transportation and thus reviewed to identify requirements for which LANL is responsible for satisfying: Order 5820.2A Radioactive Waste Management; Order 1540.1 Materials Transportation and Traffic Management; and Order 5480.3 Safety Requirements for the Packaging and Transportation of Hazardous Materials, Hazardous Substances, and Hazardous Wastes. The LANL On-Site Transportation Manual and the Administrative Requirements contained in the LANL Environment, Safety, and Health Manual were reviewed to verify that each of the requirements identified through the review of the Orders and 10 CFR Part 71 were being satisfied. The following Administrative Requirements were considered in this task: Shipment of Radioactive Materials; Radioactive Liquid Waste; Low-Level Radioactive Solid Waste; Chemical, Hazardous, and Mixed Waste; Polychlorinated Biphenyls; and Transuranic (TRU) Solid Waste

  19. Transportation and the environment.

    NARCIS (Netherlands)

    Banister, D.; Anderton, K.; Bonilla, D.; Givoni, M.; Schwanen, T.

    2011-01-01

    The growth of CO2-intensive transport, mobility and the impact of transport on the environment are reviewed. The recent global exponential growth in transport is unsustainable and must end unless the transport sector can decarbonize. The paper examines solutions for low-carbon transport systems; the

  20. Safe transport of radioactive material. 3. ed

    International Nuclear Information System (INIS)

    The IAEA has developed a standardized approach to transport safety training as a means of helping Member States to implement the Transport Regulations. The training manual is an anchor of this standardized approach to training: it contains all the topics presented in the sequential order recommended by the IAEA for the student to gain a thorough understanding of the body of knowledge that is needed to ensure that radioactive material ranked as Class 7 in the United Nations' nomenclature for dangerous goods - is transported safely. The explanations in the text refer, where needed, to the appropriate requirements in the IAEA's Transport Regulations; additional useful information is also provided. Thus, the training manual in addition to the Transport Regulations and their supporting documents is used by the IAEA as the basis for delivering all of its training courses on the safe transport of radioactive material. Enclosed with the training manual is a CD-ROM that contains the text of the manual as well as the visual aids that are used at the IAEA's training courses. The following topics are covered: review of radioactivity and radiation; review of radiation protection principles; regulatory terminology; basic safety concepts: materials and packages; activity limits and material restrictions; selection of optimal package type; test procedures: material and packages; requirements for transport; control of material in transport; fissile material: regulatory requirements and operational aspects; quality assurance; national competent authority; additional regulatory constraints for transport; international liability and insurance; emergency planning and preparedness; training; services provided by the IAEA

  1. Towards 100 gigabit carrier ethernet transport networks

    DEFF Research Database (Denmark)

    Rasmussen, Anders; Zhang, Jiang; Yu, Hao;

    2010-01-01

    Ethernet as a transport technology has, up to now, lacked the features such as network layer architecture, customer separation and manageability that carriers require for wide-scale deployment. However, with the advent of PBB-TE and TMPLS, it is now possible to use Ethernet as a transport...

  2. Transport processes of the legume symbiosome membrane

    Directory of Open Access Journals (Sweden)

    Victoria C Clarke

    2014-12-01

    Full Text Available The symbiosome membrane (SM is a physical barrier between the host plant and nitrogen-fixing bacteria in the legume-rhizobium symbiosis, and represents a regulated interface for the movement of solutes between the symbionts that is under plant control. The primary nutrient exchange across the SM is the transport of a carbon energy source from plant to bacteroid in exchange for fixed nitrogen. At a biochemical level two channels have been implicated in movement of fixed nitrogen across the SM and a uniporter that transports monovalent dicarboxylate ions has been characterized that would transport fixed carbon. The aquaporin NOD26 may provide a channel for ammonia, but the genes encoding the other transporters have not been identified. Transport of several other solutes, including calcium and potassium, have been demonstrated in isolated symbiosomes, and genes encoding transport systems for the movement of iron, nitrate, sulfate and zinc in nodules have been identified. However, definitively matching transport activities with these genes has proved difficult and many further transport processes are expected on the SM to facilitate the movement of nutrients between the symbionts. Recently, work detailing the SM proteome in soybean has been completed, contributing significantly to the database of known SM proteins. This represents a valuable resource for the identification of transporter protein candidates, some of which may correspond to transport processes previously described, or to novel transport systems in the symbiosis. Putative transporters identified from the proteome include homologues of transporters of sulfate, calcium, peptides and various metal ions. Here we review current knowledge of transport processes of the SM and discuss the requirements for additional transport routes of other nutrients exchanged in the symbiosis, with a focus on transport systems identified through the soybean SM proteome.

  3. 75 FR 50730 - Approval and Promulgation of Air Quality Implementation Plans; Indiana; Transportation Conformity...

    Science.gov (United States)

    2010-08-17

    ...; Transportation Conformity Consultation Requirement AGENCY: Environmental Protection Agency (EPA). ACTION... (SIP) submitted on June 4, 2010. This revision consists of transportation conformity criteria and... Transportation Conformity regulations. DATES: Comments must be received on or before September 16,...

  4. HUMAN RESOURCES MANAGEMENT IN SLOVAK TRANSPORT COMPANY

    OpenAIRE

    Farkašová, V.; Klieštik, T.

    2005-01-01

    The article deals with human resource management in a transport company. It shortly examines the following: quality management, the functions of human resources management and requirements to the human resources personnel.

  5. The BBSome controls IFT assembly and turnaround in cilia

    OpenAIRE

    WEI, QING; Zhang, Yuxia; Li, Yujie; Zhang, Qing; Ling, Kun; Hu, Jinghua

    2012-01-01

    The bidirectional movement of intraflagellar transport (IFT) particles, which are composed of motors, IFT-A and IFT-B subcomplexes, and cargos, is required for cilia biogenesis and signaling 1, 2 . A successful IFT cycle depends on the massive IFT particle to be properly assembled at the ciliary base and turned around from anterograde to retrograde transport at the ciliary tip. However, how IFT assembly and turnaround are regulated in vivo remains elusive. From a whole-genome mutagenesis scre...

  6. The National Neonatal Transport Programme (NNTP) 2004-2009.

    LENUS (Irish Health Repository)

    Noone, D

    2011-09-01

    A retrospective analysis of all National Neonatal Transport Programme (NNTP) transport data from 2004-2009 was performed. 1621 transports were conducted during this period with a yearly average of 271. The majority (96%) were ground transports. 1118 (69%) were forward transfers. Of the 446 (27.5%) retrotransfers, 411 (91%.) were to tertiary centres. When transported, 592 infants (36.5%) were <48 hours old and 770 (47.5%) were <1 week old. 902 transports (55%) involved infants <32 wks birth-gestation and 720 (44%) involved infants weighing <1500gms. Transport for management of patent ductus arteriosus accounted for 357 (22%). The average mobilisation time was 34 minutes. 54% of transports were completed after scheduled service hours. The NNTP currently transports similar numbers of critically ill infants during its 8hr service compared with neonatal transport services that operate 24hr services. Performing PDA ligations in a tertiary neonatal unit would significantly reduce the number of neonatal transports required annually.

  7. Optimal transport on supply-demand networks

    OpenAIRE

    Chen, Yu-Han; Wang, Bing-Hong; Zhao, Li-Chao; Zhou, Changsong; Zhou, Tao

    2009-01-01

    Previously, transport networks are usually treated as homogeneous networks, that is, every node has the same function, simultaneously providing and requiring resources. However, some real networks, such as power grid and supply chain networks, show a far different scenario in which the nodes are classified into two categories: the supply nodes provide some kinds of services, while the demand nodes require them. In this paper, we propose a general transport model for those supply-demand networ...

  8. HMPT: Hazardous Waste Transportation Live 27928, Test 27929

    Energy Technology Data Exchange (ETDEWEB)

    Simpson, Lewis Edward [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

    2016-03-17

    HMPT: Hazardous Waste Transportation (Live 27928, suggested one time and associated Test 27929, required initially and every 36 months) addresses the Department of Transportation (DOT) function-specific training requirements of the hazardous materials packagings and transportation (HMPT) Los Alamos National Laboratory (LANL) lab-wide training. This course addresses the requirements of the DOT that are unique to hazardous waste shipments. Appendix B provides the Title 40 Code of Federal Regulations (CFR) reference material needed for this course.

  9. HMPT: Hazardous Waste Transportation Live 27928, Test 27929

    International Nuclear Information System (INIS)

    HMPT: Hazardous Waste Transportation (Live 27928, suggested one time and associated Test 27929, required initially and every 36 months) addresses the Department of Transportation (DOT) function-specific training requirements of the hazardous materials packagings and transportation (HMPT) Los Alamos National Laboratory (LANL) lab-wide training. This course addresses the requirements of the DOT that are unique to hazardous waste shipments. Appendix B provides the Title 40 Code of Federal Regulations (CFR) reference material needed for this course.

  10. Functional genomics of intraflagellar transport-associated proteins in C. elegans.

    Science.gov (United States)

    Inglis, Peter N; Blacque, Oliver E; Leroux, Michel R

    2009-01-01

    The nematode Caenorhabditis elegans presents numerous advantages for the identification and molecular analysis of intraflagellar transport (IFT)-associated proteins, which play a critical role in the formation of cilia. Many proteins were first described as participating in IFT in this organism, including IFTA-1 (IFT121), DYF-1 (fleer/IFT70), DYF-2 (IFT144), DYF-3 (Qilin), DYF-11 (MIP-T3/IFT54), DYF-13, XBX-1 (dynein light intermediate chain), XBX-2 (dynein light chain), CHE-13 (IFT57/HIPPI), orthologs of Bardet-Biedl syndrome proteins, and potential regulatory protein, IFTA-2 (RABL5/IFT22). Transgenic animals bearing green fluorescent protein (GFP)-tagged proteins can be generated with ease, and in vivo imaging of IFT in both wild-type and cilia mutant strains can be performed quickly. The analyses permit detailed information on the localization and dynamic properties (velocities along the ciliary axoneme) of the relevant proteins, providing insights into their potential functions in processes such as anterograde and retrograde transport and cilium formation, as well as association with distinct modules of the IFT machinery (e.g., IFT subcomplexes A or B). Behavioral studies of the corresponding IFT-associated gene mutants further enable an understanding of the ciliary role of the proteins-e.g., in chemosensation, lipid homeostasis, lifespan control, and signaling-in a multicellular animal. In this chapter, we discuss how C. elegans can be used for the identification and characterization of IFT-associated proteins, focusing on methods for the generation of GFP-tagged IFT reporter strains, time-lapse microscopy, and IFT rate measurements. PMID:20409822

  11. Overview of nuclear materials transportation

    International Nuclear Information System (INIS)

    This presentation is an overview of transportation as it relates to one specific type of material, low specific activity (LSA) material. It is the predominant type of material that fits into the low-level waste category. An attempt is made to discuss how LSA is regulated, setting forth the requirements. First the general scheme of regulations are reviewed. In addition future changes in the regulations which will affect transportation of LSA materials and, which quite likely, will have an impact on R and D needs in this area are presented

  12. Saxton Transportation Operations Laboratory

    Data.gov (United States)

    Federal Laboratory Consortium — The Saxton Transportation Operations Laboratory (Saxton Laboratory) is a state-of-the-art facility for conducting transportation operations research. The laboratory...

  13. Charge transport in organic crystals

    Energy Technology Data Exchange (ETDEWEB)

    Ortmann, Frank

    2009-07-01

    The understanding of charge transport is one of the central goals in the research on semiconducting crystals. For organic crystals this is particularly complicated due to the strength of the electron-phonon interaction which requires the description of a seamless transition between the limiting cases of a coherent band-transport mechanism and incoherent hopping. In this thesis, charge transport phenomena in organic crystals are studied by theoretical means. A theory for charge transport in organic crystals is developed which covers the whole temperature range from low T, where it reproduces an expression from the Boltzmann equation for band transport, via elevated T, where it generalizes Holstein's small-polaron theory to finite bandwidths, up to high T, for which a temperature dependence equal to Marcus' electron-transfer theory is obtained. Thereby, coherent band transport and thermally induced hopping are treated on equal footing while simultaneously treating the electron-phonon interaction non-perturbatively. By avoiding the approximation of narrow polaron bands the theory allows for the description of large and small polarons and serves as a starting point for computational studies. The theoretical description is completed by using ab initio material parameters for the selected crystals under study. These material parameters are taken from density functional theory calculations for durene, naphthalene, and guanine crystals. Besides the analysis of the transport mechanism, special focus is put on the study of the relationship between mobility anisotropy and structure of the crystals. This study is supported by a 3D-visualization method for the transport channels in such crystals which has been derived in this thesis. (orig.)

  14. Chamber transport for heavy ion fusion

    International Nuclear Information System (INIS)

    A brief review is given of research on chamber transport for HIF (heavy ion fusion) dating from the first HIF Workshop in 1976 to the present. Chamber transport modes are categorized into ballistic transport modes and channel-like modes. Four major HIF reactor studies are summarized (HIBALL-II, HYLIFE-II, Prometheus-H, OSIRIS), with emphasis on the chamber transport environment. In general, many beams are used to provide the required symmetry and to permit focusing to the required small spots. Target parameters are then discussed, with a summary of the individual heavy ion beam parameters required for HIF. The beam parameters are then classified as to their line charge density and perveance, with special emphasis on the perveance limits for radial space charge spreading, for the space charge limiting current, and for the magnetic (Alfven) limiting current. The major experiments on ballistic transport (SFFE, Sabre beamlets, GAMBLE II, NTX, NDCX) are summarized, with specific reference to the axial electron trapping limit for charge neutralization. The major experiments on channel-like transport (GAMBLE II channel, GAMBLE II self-pinch, LBNL channels, GSI channels) are discussed. The status of current research on HIF chamber transport is summarized, and the value of future NDCX-II transport experiments for the future of HIF is noted

  15. Chamber transport for heavy ion fusion

    Energy Technology Data Exchange (ETDEWEB)

    Olson, Craig L., E-mail: clolson66@msn.com

    2014-01-01

    A brief review is given of research on chamber transport for HIF (heavy ion fusion) dating from the first HIF Workshop in 1976 to the present. Chamber transport modes are categorized into ballistic transport modes and channel-like modes. Four major HIF reactor studies are summarized (HIBALL-II, HYLIFE-II, Prometheus-H, OSIRIS), with emphasis on the chamber transport environment. In general, many beams are used to provide the required symmetry and to permit focusing to the required small spots. Target parameters are then discussed, with a summary of the individual heavy ion beam parameters required for HIF. The beam parameters are then classified as to their line charge density and perveance, with special emphasis on the perveance limits for radial space charge spreading, for the space charge limiting current, and for the magnetic (Alfven) limiting current. The major experiments on ballistic transport (SFFE, Sabre beamlets, GAMBLE II, NTX, NDCX) are summarized, with specific reference to the axial electron trapping limit for charge neutralization. The major experiments on channel-like transport (GAMBLE II channel, GAMBLE II self-pinch, LBNL channels, GSI channels) are discussed. The status of current research on HIF chamber transport is summarized, and the value of future NDCX-II transport experiments for the future of HIF is noted.

  16. The Edge supersonic transport

    Science.gov (United States)

    Agosta, Roxana; Bilbija, Dushan; Deutsch, Marc; Gallant, David; Rose, Don; Shreve, Gene; Smario, David; Suffredini, Brian

    1992-01-01

    As intercontinental business and tourism volumes continue their rapid expansion, the need to reduce travel times becomes increasingly acute. The Edge Supersonic Transport Aircraft is designed to meet this demand by the year 2015. With a maximum range of 5750 nm, a payload of 294 passengers and a cruising speed of M = 2.4, The Edge will cut current international flight durations in half, while maintaining competitive first class, business class, and economy class comfort levels. Moreover, this transport will render a minimal impact upon the environment, and will meet all Federal Aviation Administration Part 36, Stage III noise requirements. The cornerstone of The Edge's superior flight performance is its aerodynamically efficient, dual-configuration design incorporating variable-geometry wingtips. This arrangement combines the benefits of a high aspect ratio wing at takeoff and low cruising speeds with the high performance of an arrow-wing in supersonic cruise. And while the structural weight concerns relating to swinging wingtips are substantial, The Edge looks to ever-advancing material technologies to further increase its viability. Heeding well the lessons of the past, The Edge design holds economic feasibility as its primary focus. Therefore, in addition to its inherently superior aerodynamic performance, The Edge uses a lightweight, largely windowless configuration, relying on a synthetic vision system for outside viewing by both pilot and passengers. Additionally, a fly-by-light flight control system is incorporated to address aircraft supersonic cruise instability. The Edge will be produced at an estimated volume of 400 aircraft and will be offered to airlines in 2015 at $167 million per transport (1992 dollars).

  17. Optimal transport on supply-demand networks

    CERN Document Server

    Chen, Yu-Han; Zhao, Li-Chao; Zhou, Changsong; Zhou, Tao

    2009-01-01

    Previously, transport networks are usually treated as homogeneous networks, that is, every node has the same function, simultaneously providing and requiring resources. However, some real networks, such as power grid and supply chain networks, show a far different scenario in which the nodes are classified into two categories: the supply nodes provide some kinds of services, while the demand nodes require them. In this paper, we propose a general transport model for those supply-demand networks, associated with a criterion to quantify their transport capacities. In a supply-demand network with heterogenous degree distribution, its transport capacity strongly depends on the locations of supply nodes. We therefore design a simulated annealing algorithm to find the optimal configuration of supply nodes, which remarkably enhances the transport capacity, and outperforms the degree target algorithm, the betweenness target algorithm, and the greedy method. This work provides a start point for systematically analyzin...

  18. Truck transportation of radioactive materials

    International Nuclear Information System (INIS)

    Analytical models in RADTRAN II are used to calculate risks to population subgroups such as people along transport routes, people at stops, and crewman. The stops model, which calculates the dose to persons adjacent to the transport vehicle while it is stopped, frequently provides the largest contribution to incident-free radiological impacts. Components such as distances from the vehicle containing radioactive material to nearby people at stops, stop duration, and number of crew members are required for the stops model as well as other incident-free models. To provide supporting data for RADTRAN II based on operational experience, selected truck shipments of radioactive material were observed from origin to destination. Other important aspects of this program were to correlate package size to effective shipment transport index (TI) using radiological surveys and to characterize population distributions and proximities of people to the shipment at a generic truck stop

  19. Modelling freight transport

    NARCIS (Netherlands)

    Tavasszy, L.A.; Jong, G. de

    2014-01-01

    Freight Transport Modelling is a unique new reference book that provides insight into the state-of-the-art of freight modelling. Focusing on models used to support public transport policy analysis, Freight Transport Modelling systematically introduces the latest freight transport modelling approache

  20. Predicting transportation routes for radioactive wastes

    International Nuclear Information System (INIS)

    Oak Ridge National Laboratory (ORNL) has been involved in transportation logistics of radioactive wastes as part of the overall waste transportation program. A Spent Fuel Logistics Model (SFLM), was developed to predict overall material balances representing the flow of spent fuel assemblies from reactors to away-from-reactor storage facilities and/or to federal repositories. The transportation requirements to make these shipments are also itemized. The next logical step in the overall transportation project was the development of a set of computer codes which would predict likely transportation routes for waste shipments. Two separate routing models are now operational at ORNL. Routes for truck transport can be estimated with the HIGHWAY program, and rail and barge routes can be predicted with the INTERLINE model. This paper discusses examples of the route estimates and applications of the routing models

  1. Framework for Sustainability Assessment by Transportation Agencies

    DEFF Research Database (Denmark)

    Ramani, Tara Lakshmi; Zietsman, Josias; Gudmundsson, Henrik;

    2011-01-01

    promotes the consideration of transportation from a holistic sustainable development perspective. The framework defines broadly applicable transportation goals that can be broken down into a menu of objectives and performance measures to cover various transportation contexts. The framework is also designed...... transportation agencies with the tools required to understand what sustainability means and incorporate sustainability into the organizational culture. This approach and method can also help agencies lay the groundwork for the use of performance measures so the agencies can progress toward sustainability goals...... and outcomes. The framework development process was an extension of findings from literature review, case studies, and interviews conducted as part of ongoing research under the NCHRP project Sustainability Performance Measures for State Departments of Transportation and Other Transportation Agencies...

  2. Cytoskeletal network morphology regulates intracellular transport dynamics

    CERN Document Server

    Ando, David; Huang, Kerwyn Casey; Gopinathan, Ajay

    2016-01-01

    Intracellular transport is essential for maintaining proper cellular function in most eukaryotic cells, with perturbations in active transport resulting in several types of disease. Efficient delivery of critical cargos to specific locations is accomplished through a combination of passive diffusion and active transport by molecular motors that ballistically move along a network of cytoskeletal filaments. Although motor-based transport is known to be necessary to overcome cytoplasmic crowding and the limited range of diffusion within reasonable time scales, the topological features of the cytoskeletal network that regulate transport efficiency and robustness have not been established. Using a continuum diffusion model, we observed that the time required for cellular transport was minimized when the network was localized near the nucleus. In simulations that explicitly incorporated network spatial architectures, total filament mass was the primary driver of network transit times. However, filament traps that r...

  3. 湖北交通运输“十二五”教育与培训发展形势与需求分析%Analysis on Education & Training Developing Trends and Requirements for Hubei Transportation "12th Five"

    Institute of Scientific and Technical Information of China (English)

    罗星海

    2011-01-01

    "Give priority to education,vigorously develop vocational education,and accelerate the development of continuing education" proposed new requirements on education and training.Vocational education reform and development shows a new trend and the development of modern transport leads to a new challenge on the education and training,what's more,Hubei highway and waterway transport development has brought new opportunities for education and training.To adjust the transport structure,change the mode of development,adapt to rapid development and enhance job skills,build a harmonious transportation industry,set the industry image,enhance service capabilities,and also to achieve scientific development,it is essential to strengthen the training of qualified personnel,enhance industrial innovative ability,speed up professionals and high-quality skilled personnel training,and enhance the quality of industry labor force.%"优先发展教育、大力发展职业教育、加快发展继续教育"为教育与培训提出了新要求,职业教育改革与发展呈现出新趋势,发展现代交通运输业对教育与培训提出了新挑战,湖北公路水路交通运输发展为教育与培训带来了新机遇。调整运输结构,转变发展方式,适应快速发展,提高岗位技能,构建和谐交通,树立行业形象,提升服务能力,实现科学发展,需要强化干部人才队伍培养、加强行业创新能力培养、加快专业技术人才和高素质技能型专门人才培养、提升行业劳动者队伍素质。

  4. Intestinal Phosphate Transport

    OpenAIRE

    Sabbagh, Yves; Giral, Hector; Caldas, Yupanqui; Levi, Moshe; Schiavi, Susan C.

    2011-01-01

    Phosphate is absorbed in the small intestine by at least two distinct mechanisms: paracellular phosphate transport which is dependent on passive diffusion and active transport which occurs through the sodium-dependent phosphate co-transporters. Despite evidence emerging for other ions, regulation of the phosphate specific paracellular pathways remains largely unexplored. In contrast, there is a growing body of evidence that active transport through the sodium-dependent phosphate co-transporte...

  5. 49 CFR Appendix II to Part 805 - Employees Required To Submit Statements

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 7 2010-10-01 2010-10-01 false Employees Required To Submit Statements II Appendix II to Part 805 Transportation Other Regulations Relating to Transportation (Continued) NATIONAL...—Employees Required To Submit Statements Statements of employment and financial interests are required of...

  6. 14 CFR 121.378 - Certificate requirements.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Certificate requirements. 121.378 Section 121.378 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED... REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Maintenance, Preventive Maintenance, and...

  7. 49 CFR 451.25 - Required information.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 6 2010-10-01 2010-10-01 false Required information. 451.25 Section 451.25....25 Required information. (a) The safety approval number appearing on line 1 of the safety approval... safety approval plate on the freight container provided that all the information contained on...

  8. 14 CFR 147.15 - Space requirements.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Space requirements. 147.15 Section 147.15 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) SCHOOLS AND OTHER CERTIFICATED AGENCIES AVIATION MAINTENANCE TECHNICIAN SCHOOLS Certification Requirements §...

  9. 14 CFR 135.427 - Manual requirements.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Manual requirements. 135.427 Section 135.427 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED... REQUIREMENTS: COMMUTER AND ON DEMAND OPERATIONS AND RULES GOVERNING PERSONS ON BOARD SUCH AIRCRAFT...

  10. 14 CFR 121.369 - Manual requirements.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Manual requirements. 121.369 Section 121.369 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED... REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Maintenance, Preventive Maintenance, and...

  11. 14 CFR 135.435 - Certificate requirements.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Certificate requirements. 135.435 Section 135.435 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED... REQUIREMENTS: COMMUTER AND ON DEMAND OPERATIONS AND RULES GOVERNING PERSONS ON BOARD SUCH AIRCRAFT...

  12. 49 CFR 630.4 - Requirements.

    Science.gov (United States)

    2010-10-01

    ... from the National Transit Database Web site located at http://www.ntdprogram.gov. These reference... Transit Database Web site and a notice of any significant changes to the reporting requirements specified... TRANSPORTATION NATIONAL TRANSIT DATABASE § 630.4 Requirements. (a) National Transit Database Reporting...

  13. 40 CFR 205.55 - Requirements.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Requirements. 205.55 Section 205.55 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) NOISE ABATEMENT PROGRAMS TRANSPORTATION EQUIPMENT NOISE EMISSION CONTROLS Medium and Heavy Trucks § 205.55 Requirements....

  14. 14 CFR 21.613 - Recordkeeping requirements.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 1 2010-01-01 2010-01-01 false Recordkeeping requirements. 21.613 Section 21.613 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION AIRCRAFT... requirements. (a) Keeping the records. Each manufacturer holding a TSO authorization under this part shall,...

  15. 49 CFR 1503.101 - TSA requirements.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 9 2010-10-01 2010-10-01 false TSA requirements. 1503.101 Section 1503.101... Scope of Investigative and Enforcement Procedures § 1503.101 TSA requirements. (a) The investigative and enforcement procedures in this part apply to TSA's investigation and enforcement of violations of...

  16. Onsite transportation of radioactive materials at the Savannah River Site

    Energy Technology Data Exchange (ETDEWEB)

    Watkins, R.

    2015-03-03

    The Savannah River Site (SRS) Transportation Safety Document (TSD) defines the onsite packaging and transportation safety program at SRS and demonstrates its compliance with Department of Energy (DOE) transportation safety requirements, to include DOE Order 460.1C, DOE Order 461.2, Onsite Packaging and Transfer of Materials of National Security Interest, and 10 CFR 830, Nuclear Safety Management (Subpart B).

  17. Burning water: the water footprint of biofuel-based transport

    NARCIS (Netherlands)

    Gerbens-Leenes, P.W.; Hoekstra, A.Y.

    2010-01-01

    The trend towards substitution of conventional transport fuels by biofuels requires additional water. The EU aims to replace 10 percent of total transport fuels by biofuels by 2020. This study calculates the water footprint (WF) of different transport modes using bio-ethanol, biodiesel or bio-electr

  18. Expression of Thyroid Hormone Transporters in the Human Hypothalamus

    NARCIS (Netherlands)

    A. Alkemade; E.C.H. Friesema; A. Kalsbeek; D.F. Swaab; T.J. Visser; E. Fliers

    2011-01-01

    Context: Transport of thyroid hormone across the plasma membrane is required for proper thyroid hormone action and metabolism. Several specific thyroid hormone transporters have been identified capable of facilitating uptake and/or efflux of thyroid hormones. Monocarboxylate transporter (MCT)-8, MCT

  19. 29 CFR 780.911 - Preparation for transportation.

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 3 2010-07-01 2010-07-01 false Preparation for transportation. 780.911 Section 780.911... Employment in Fruit and Vegetable Harvest Transportation; Exemption From Overtime Pay Requirements Under Section 13(b)(16) Exempt Operations on Fruits Or Vegetables § 780.911 Preparation for transportation....

  20. 42 CFR 431.53 - Assurance of transportation.

    Science.gov (United States)

    2010-10-01

    ... 42 Public Health 4 2010-10-01 2010-10-01 false Assurance of transportation. 431.53 Section 431.53... Requirements § 431.53 Assurance of transportation. A State plan must— (a) Specify that the Medicaid agency will ensure necessary transportation for recipients to and from providers; and (b) Describe the methods...

  1. Engineering Study of 500 ML Sample Bottle Transportation Methods

    Energy Technology Data Exchange (ETDEWEB)

    BOGER, R.M.

    1999-08-25

    This engineering study reviews and evaluates all available methods for transportation of 500-mL grab sample bottles, reviews and evaluates transportation requirements and schedules and analyzes and recommends the most cost-effective method for transporting 500-mL grab sample bottles.

  2. Transportation technology program: Strategic plan

    Science.gov (United States)

    1991-01-01

    The purpose of this report is to define the technology program required to meet the transportation technology needs for current and future civil space missions. It is a part of an integrated plan, prepared by NASA in part in response to the Augustine Committee recommendations, to describe and advocate expanded and more aggressive efforts in the development of advanced space technologies. This expanded program will provide a technology basis for future space missions to which the U.S. aspires, and will help to regain technology leadership for the U.S. on a broader front. The six aspects of this integrated program/plan deal with focused technologies to support space sciences, exploration, transportation, platforms, and operations as well as provide a Research and Technology Base Program. This volume describes the technologies needed to support transportation systems, e.g., technologies needed for upgrades to current transportation systems and to provide reliable and efficient transportation for future space missions. The Office of Aeronautics, Exploration, and Technology (OAET) solicited technology needs from the major agency technology users and the aerospace industry community and formed a transportation technology team (appendix A) to develop a technology program to respond to those needs related to transportation technologies. This report addresses the results of that team activity. It is a strategic plan intended for use as a planning document rather than as a project management tool. It is anticipated that this document will be primarily utilized by research & technology (R&T) management at the various NASA Centers as well as by officials at NASA Headquarters and by industry in planning their corporate Independent Research and Development (IR&D) investments.

  3. Space Transportation and Destination Facilities

    Science.gov (United States)

    Smitherman, David; McClure, Wallace

    1999-01-01

    The Space Transportation and Destination Facilities section focused on space transportation vehicles-from use of existing vehicles to development of specialized transports-and on space stations, space business parks, space hotels, and other facilities in space of the kind that eventually would provide services for general public space travel (PST) and tourism. For both transportation and destination facilities, the emphasis was on the identification of various strategies to enable a realistic incremental progression in the development and acquisition of such facilities, and the identification of issues that need resolution to enable formation of viable businesses. The approach was to determine the best: (1) Strategies for general PST and tourism development through the description and analysis of a wide range of possible future scenarios. With these scenarios in mind the section then identified. (2) Key issues to be explored. (3) opportunities to eliminate barriers. (4) Recommendations for future actions. (5) Top-level requirements and characteristics for general PST and tourism systems and services that would guide the development of transportation and destination facilities.

  4. 48 CFR 728.313 - Contract clauses for insurance of transportation or transportation-related services.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Contract clauses for insurance of transportation or transportation-related services. 728.313 Section 728.313 Federal Acquisition...) USAID is required by law to include language in all its direct contracts and subcontracts ensuring...

  5. Water-transporting proteins.

    Science.gov (United States)

    Zeuthen, Thomas

    2010-04-01

    Transport through lipids and aquaporins is osmotic and entirely driven by the difference in osmotic pressure. Water transport in cotransporters and uniporters is different: Water can be cotransported, energized by coupling to the substrate flux by a mechanism closely associated with protein. In the K(+)/Cl(-) and the Na(+)/K(+)/2Cl(-) cotransporters, water is entirely cotransported, while water transport in glucose uniporters and Na(+)-coupled transporters of nutrients and neurotransmitters takes place by both osmosis and cotransport. The molecular mechanism behind cotransport of water is not clear. It is associated with the substrate movements in aqueous pathways within the protein; a conventional unstirred layer mechanism can be ruled out, due to high rates of diffusion in the cytoplasm. The physiological roles of the various modes of water transport are reviewed in relation to epithelial transport. Epithelial water transport is energized by the movements of ions, but how the coupling takes place is uncertain. All epithelia can transport water uphill against an osmotic gradient, which is hard to explain by simple osmosis. Furthermore, genetic removal of aquaporins has not given support to osmosis as the exclusive mode of transport. Water cotransport can explain the coupling between ion and water transport, a major fraction of transepithelial water transport and uphill water transport. Aquaporins enhance water transport by utilizing osmotic gradients and cause the osmolarity of the transportate to approach isotonicity. PMID:20091162

  6. PEM fuel cell bipolar plate material requirements for transportation applications

    Energy Technology Data Exchange (ETDEWEB)

    Borup, R.L.; Stroh, K.R.; Vanderborgh, N.E. [Los Alamos National Lab., NM (United States)] [and others

    1996-04-01

    Cost effective bipolar plates are currently under development to help make proton exchange membrane (PEM) fuel cells commercially viable. Bipolar plates separate individual cells of the fuel cell stack, and thus must supply strength, be electrically conductive, provide for thermal control of the fuel stack, be a non-porous materials separating hydrogen and oxygen feed streams, be corrosion resistant, provide gas distribution for the feed streams and meet fuel stack cost targets. Candidate materials include conductive polymers and metal plates with corrosion resistant coatings. Possible metals include aluminium, titanium, iron/stainless steel and nickel.

  7. 9 CFR 88.4 - Requirements for transport.

    Science.gov (United States)

    2010-01-01

    ... telephone number; (iii) The name of the auction/market, if applicable; (iv) A description of the conveyance... the equine in accordance with paragraph (a)(2) of this section; (vii) A statement of fitness to...

  8. Linac transport and acceleration

    International Nuclear Information System (INIS)

    The acceleration of intense bunches maintaining high brightness is limited both by single-particle effects, e.g., misalignments, injection errors, and rf-steering, and collective phenomena, where the effects of the longitudinal and transverse wakefield on particles within a single bunch are the most severe. The working group has considered both problems and potentials of linac acceleration from ∼50 MeV to 1 GeV for free electron laser (FEL) applications, as well as from a few Gev to 1 TeV for linear colliders. The outlook for free electron lasers is bright: no fundamental problems seem to arise in the acceleration of peak currents in excess of 100 A with small emittance and low momentum spread. The situation of linear colliders is more complex and more difficult. Two examples, one operating at 11.4 GHz, the other at 30 GHz, are used to illustrate some of the difficulties and the exceedingly tight tolerances required. Both examples are based on round beams, and thus neither benefit from the advantages of flat beams nor address the increased care required in transporting beams of very small emittance in one plane. The working group acknowledges, but did not explore, promising concepts for colliders based on RF superconductivity

  9. Types of solutions improving passenger transport interconnectivity

    Directory of Open Access Journals (Sweden)

    Monika BĄK

    2012-01-01

    Full Text Available The objective of the paper is to present different types of solutions which could improve interconnectivity of passenger transport especially within interconnections between long and short transport distance. The topic has particular relevance at the European level because the European transport networks’ role as integrated international networks is compromised by poor interconnectivity and because the next generation of European transport policies will have to be sensitive to the differences between short, medium and long-term transport markets and the market advantages of each transport mode. In this context, a realistic assessment of intermodal opportunities is a key ingredient to future policy development.Effective interconnection requires the provision of integrated networks and services which are attractive to potential users and this is likely to require co-operation between a range of authorities and providers in the public and private sectors and may necessitate a wider vision than might otherwise prevail.The paper is based on the results of the project realised by the team of the University of Gdansk in the EU funded 7 Framework Programme - INTERCONNECT (Interconnection between short- and long-distance transport networks with partners in the UK, Germany, Denmark, Poland, Spain and Italy. Different types of solutions will be summarized in the paper including e.g. local link infrastructure solutions, improved local public transport services, improvements at the interchange, solutions involving improved procedures for check-in or luggage transfer & documentation, pricing and ticketing solutions, solutions involving marketing, information and sales.

  10. Salt Repository Project transportation program plan

    International Nuclear Information System (INIS)

    The Salt Repository Project (SRP) has the responsibility to develop a comprehensive transportation program plan (TrPP) that treats the transportation of workers, supplies, and high-level radioactive waste to the site and the transportation of salt, low-level, and transuranic wastes from the site. The TrPP has developed a systematic approach to transportation which is directed towards satisfying statutes, regulations, and directives and is guided by a hierarchy of specific functional requirements, strategies, plans, and reports. The TrPP identifies and develops the planning process for transportation-related studies and provides guidance to staff in performing and documenting these activities. The TrPP also includes an explanation of the responsibilities of the organizational elements involved in these transportation studies. Several of the report chapters relate to identifying routes for transporting nuclear waste to the site. These include a chapter on identifying an access corridor for a new rail route leading to the site, identifying and evaluating emergency-response preparedness capabilities along candidate routes in the state, and identifying alternative routes from the state border, ports, or in-state reactors to the site. The TrPP also includes plans for identifying salt disposal routes and a discussion of repository/transportation interface requirements. 89 refs., 6 figs

  11. INTERMODAL TRANSPORT IN EUROPE - OPPORTUNITIES THROUGH INNOVATION

    Directory of Open Access Journals (Sweden)

    Norbert Wagener

    2014-12-01

    Full Text Available Background: Freight transport volume in ton-km in OECD countries will grow considerably up to 150 to 230 % in 2050 compared to 2010. Although the EU policy aims to shift 30% of road freight over 300 km to other modes such as rail or waterborne transport by 2030 the recent trends show a stable modal split of road at approx. 75%. Conventional intermodal transport on the major European routes has shown a steady but only limited organic growth through recent years. Therefore, new innovative concepts for intermodal transport and for the shift from road to rail are needed. Methods: Definitions of intermodal transport have been clarified and the development of combined transport in Europe and in Germany and Poland in particular has been analyzed on the basis of available data sources. New innovative concepts for intermodal transport have been identified on the basis of desk research, recent relevant projects (RETRACK, SCANDRIA, Rail Baltica and market intelligence. Results: The analysis leads to the conclusion that new innovative concepts in intermodal transport comprise new forms of organization as well as new technologies and new routes. The following three innovations to facilitate the shift from road to rail by intermodal transport are being introduced and discussed: multimodal operation of ocean carriers in maritime hinterland transportation, innovative handling technologies for non-crane able trainers and freight corridors for long distance intermodal transport within the TEN-T network and on the Europe-Asia corridor. Conclusions: Further accelerated growth in the shift from road to rail through intermodal transport requires new innovative concepts beyond the traditional combined transport in Western Europe. Three promising innovative concepts have been introduced. Further research is needed and should be focused on financial and economic appraisal as well as on the effectiveness of state intervention policies. 

  12. Transportation Technology: Rail Transport and Logistics

    Science.gov (United States)

    Lang, Aaron B.

    2011-01-01

    Transportation can simply be defined as the movement of goods, services, and people from one location to another. Without an efficient means to transport goods from place to place, the economy would be nothing like it is today. Throughout the history of the United States, American railroads have paved the way toward creating a nation of great…

  13. 49 CFR 376.12 - Written lease requirements.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 5 2010-10-01 2010-10-01 false Written lease requirements. 376.12 Section 376.12... ADMINISTRATION, DEPARTMENT OF TRANSPORTATION FEDERAL MOTOR CARRIER SAFETY REGULATIONS LEASE AND INTERCHANGE OF VEHICLES Leasing Regulations § 376.12 Written lease requirements. Except as provided in the exemptions...

  14. 14 CFR 61.153 - Eligibility requirements: General.

    Science.gov (United States)

    2010-01-01

    ... (CONTINUED) AIRMEN CERTIFICATION: PILOTS, FLIGHT INSTRUCTORS, AND GROUND INSTRUCTORS Airline Transport Pilots § 61.153 Eligibility requirements: General. To be eligible for an airline transport pilot certificate... rating issued under this part; (2) Meet the military experience requirements under § 61.73 of this...

  15. Tourism and the Problems of Developing Transport Infrastructure in Ukraine

    Directory of Open Access Journals (Sweden)

    Natalija Tkachenko

    2011-04-01

    Full Text Available The main problems, associated with the development of a transport network in Ukraine and the need for complex evaluation of the interests of the states, seeking international cooperation in creating transport corridors for transporting tourists and determining the position of Ukraine in this cooperation, are considered. The main requirements to design of highways and transport infrastructure are defined. Article in Russian

  16. The Analysis of Sustainable Transport System Development in Lithuania

    OpenAIRE

    Jurgita Barysienė; Eglė Speičytė

    2011-01-01

    Recently, sustainable development, as well as sustainable transport, have become the guiding principles of human activities. Transport is of special importance for economic development of Lithuania, therefore a sustainable transport system is required for achieving major goals of sustainable development. The paper discusses general aims of the European Union and Lithuania’s sustainable development strategies and the role of sustainable transport in this common development. Moreover, environme...

  17. Education and training in transport of radioactive material; Educacao e treinamento em transporte de material radioativo

    Energy Technology Data Exchange (ETDEWEB)

    Carvalho, Bruno Natanael; Pastura, Valeria da Fonseca e Silva; Mattar, Patricia; Dias, Carlos R. [Comissao Nacional de Energia Nuclear (CNEN), Rio de Janeiro, RJ (Brazil)

    2013-07-01

    This paper presents the approach adopted by the Department of Transportation of the Brazilian National Nuclear Energy Commission - CNEN, in the creation of the course of education and training distance for transport companies, as well as for national institutions directly involved with the theme transportation of radioactive materials. The course will consist of 20 modules containing exercises and further assessment of learning, and enable participants to understand the regulatory terminology, assimilating the philosophy of nuclear and radiation safety, prepare the shipment and identify and fill the complete documents required in an operation transport.

  18. Oxygen transport membrane

    DEFF Research Database (Denmark)

    2015-01-01

    The present invention relates to a novel composite oxygen transport membrane as well as its preparation and uses thereof.......The present invention relates to a novel composite oxygen transport membrane as well as its preparation and uses thereof....

  19. Transportation Management Workshop: Proceedings

    Energy Technology Data Exchange (ETDEWEB)

    1993-10-01

    This report is a compilation of discussions presented at the Transportation Management Workshop held in Gaithersburg, Maryland. Topics include waste packaging, personnel training, robotics, transportation routing, certification, containers, and waste classification.

  20. Small Satellite Transporter Project

    Data.gov (United States)

    National Aeronautics and Space Administration — The primary objective is to determine whether this small satellite transporter is capable of transporting at least four 6U CubeSats is possible for a given set of...

  1. Speeding up Transportation

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    @@ 2007 was an excellent year for the transportation industry, marked by high speed railway transportation, development of the national expressway network and launch of the Chang'e lunar probe satellite.

  2. Transportation Management Workshop: Proceedings

    International Nuclear Information System (INIS)

    This report is a compilation of discussions presented at the Transportation Management Workshop held in Gaithersburg, Maryland. Topics include waste packaging, personnel training, robotics, transportation routing, certification, containers, and waste classification

  3. Transport Properties and Transport Phenomena in Casting Nickel Superalloys

    Science.gov (United States)

    Felicelli, S. D.; Sung, P. K.; Poirier, D. R.; Heinrich, J. C.

    1998-11-01

    Nickel superalloys that are used in the high-temperature regions of gas-turbine engines are cast by directional solidification (DS). In the DS processes, the castings are cooled from below, and three zones exist during solidification: (1) an all-solid zone at the bottom, (2) a "mushy zone" that is comprised of solid and liquid material, and (3) an overlying all-liquid zone. Computer simulations can be useful in predicting the complex transport phenomena that occur during solidification, but realistic simulations require accurate values of the transport properties. In addition to transport properties, the thermodynamic equilibria between the solid and liquid during solidification must also be known with reasonable accuracy. The importance of using reasonably accurate estimations of the transport properties is illustrated by two-dimensional simulations of the convection during solidification and the coincidental macrosegregation in the DS castings of multicomponent Ni-base alloys. In these simulations, we examine the sensitivity of the calculated results to measured partition ratios, thermal expansion coefficients, and viscosities that are estimated by regression analyses and correlations of existing property data.

  4. The WIPP transportation system: Dedicated to safety

    International Nuclear Information System (INIS)

    When developing a transportation system to transport transuranic (TRU) waste from ten widely-dispersed generator sites, the Department of Energy (DOE) recognized and addressed many challenges. Shipments of waste to the Waste Isolation Pilot Plant (WIPP) were to cover a twenty-five year period and utilize routes covering over twelve thousand miles in twenty-three states. Enhancing public safety by maximizing the payload, thus reducing the number of shipments, was the primary objective. To preclude the requirement for overweight permits, the DOE started with a total shipment weight limit of 80,000 pounds and developed an integrated transportation system consisting of a Type ''B'' package to transport the material, a lightweight tractor and trailer, stringent driver requirements, and a shipment tracking system referred to as ''TRANSCOM''

  5. Relativistic diffusive transport

    OpenAIRE

    Haba, Z.

    2009-01-01

    We discuss transport equations resulting from relativistic diffusions in the proper time. We show that a solution of the transport equation can be obtained from the solution of the diffusion equation by means of an integration over the proper time. We study the stochastic processes solving the relativistic diffusion equation and the relativistic transport equation. We show that the relativistic transport equation for massive particles in the light cone coordinates and for massless particles i...

  6. Reverse cholesterol transport revisited

    Institute of Scientific and Technical Information of China (English)

    Astrid; E; van; der; Velde

    2010-01-01

    Reverse cholesterol transport was originally described as the high-density lipoprotein-mediated cholesterol flux from the periphery via the hepatobiliary tract to the intestinal lumen, leading to fecal excretion. Since the introduction of reverse cholesterol transport in the 1970s, this pathway has been intensively investigated. In this topic highlight, the classical reverse cholesterol transport concepts are discussed and the subject reverse cholesterol transport is revisited.

  7. TRANSPORTATION INFORMATION CLEARINGHOUSE

    OpenAIRE

    Allen, Karin; DiJohn, Joseph; Misek, Shamus

    2005-01-01

    The Transportation Information Clearinghouse (TIC) Project was the result of collaboration among the Regional Transportation Authority, the Workforce Boards of Metropolitan Chicago and the Urban Transportation Center (UTC) at the University of Illinois at Chicago (UIC). The primary objective of the project was to identify privatelyprovided, employer-based, non-traditional transportation services in operation as well as specific information about these services in order for employers, Workforc...

  8. A small peptide sequence is sufficient for initiating kinesin-1 activation through part of TPR region of KLC1.

    Science.gov (United States)

    Kawano, Takanori; Araseki, Masahiko; Araki, Yoichi; Kinjo, Masataka; Yamamoto, Tohru; Suzuki, Toshiharu

    2012-06-01

    Kinesin-1 anterogradely transports vesicles containing cargo proteins when a protein-protein interaction activates it from an inhibited state. The C-terminal cytoplasmic region of kinesin-1 cargo protein Alcadeinα (Alcα) interacts with the KLC1 subunit's tetratricopeptide repeat (TPR) region, activating kinesin-1's association with vesicles and anterograde transport. We found that either of two 10-amino-acid WD motifs in Alcα cytoplasmic region was necessary and sufficient to initiate this activation. An artificial transmembrane protein containing either WD motif induced kinesin-1's vesicular association and anterograde transport in a KLC-dependent manner, even in the normally inhibiting presence of excess KLC1, thus allowing us to analyze the KLC1 TPR-WD functional interaction in detail in vivo. A part of TPR region was dispensable for the WD motifs' activation of kinesin-1 and transport, indicating that only part of the TPR structure is required for this function in vivo. For a different kinesin-1 cargo protein, JIP1, an 11-amino-acid C-terminal region was sufficient to recruit KLC1 to vesicles, but did not activate transport. These observations suggest that structurally different TPR-interacting peptides may have different effects on kinesin-1. This mechanism may partly explain how kinesin-1 can organize the transport of a wide variety of cargo molecules.

  9. NATURAL GAS TRANSPORTATION

    OpenAIRE

    Stanis³aw Brzeziñski

    2007-01-01

    In the paper, Author presents chosen aspects of natural gas transportation within global market. Natural gas transportation is a technicaly complicated and economicly expensive process; in infrastructure construction and activities costs. The paper also considers last and proposed initiatives in natural gas transportation.

  10. Particle Transport in Therapeutic Magnetic Fields

    Science.gov (United States)

    Puri, Ishwar K.; Ganguly, Ranjan

    2014-01-01

    Iron oxide magnetic nanoparticles, in ferrofluids or as magnetic microspheres, offer magnetic maneuverability, biochemical surface functionalization, and magnetic relaxation under the influence of an alternating field. The use of these properties for clinical applications requires an understanding of particles, forces, and scalar transport at various length scales. This review explains the behavior of magnetic nano- and microparticles during magnetic drug targeting and magnetic fluid hyperthermia, and the microfluidic transport of these particles in bioMEMS (biomedical microelectromechanical systems) devices for ex vivo therapeutic and diagnostic applications. Magnetic particle transport, the momentum interaction of these particles with a host fluid in a flow, and thermal transport in a particle-infused tissue are characterized through the governing electrodynamic, hydrodynamic, and scalar transport equations.

  11. JIT-transportation problem and its algorithm

    Science.gov (United States)

    Bai, Guozhong; Gan, Xiao-Xiong

    2011-12-01

    This article introduces the (just-in-time) JIT-transportation problem, which requires that all demanded goods be shipped to their destinations on schedule, at a zero or minimal destination-storage cost. The JIT-transportation problem is a special goal programming problem with discrete constraints. This article provides a mathematical model for such a transportation problem and introduces the JIT solution, the deviation solution, the JIT deviation, etc. By introducing the B(λ)-problem, this article establishes the equivalence between the optimal solutions of the B(λ)-problem and the optimal solutions of the JIT-transportation problem, and then provides an algorithm for the JIT-transportation problems. This algorithm is proven mathematically and is also illustrated by an example.

  12. Technology evaluation for time sensitive data transport

    DEFF Research Database (Denmark)

    Wessing, Henrik; Breach, Tony; Colmenero, Alberto;

    . The NREN communities must provide underlying network infrastructures and transport technologies to facilitate ser-vices with such requirements to the network. In this paper we investigate and evaluate circuit and packet based transport technologies from classic best effort IP over MPLS flavours, Provider...... Backbone Bridging (PBB), “Transparent Interconnect of Lots of Links” (TRILL) to Optical Transport Network (OTN) and SDH. The transport technologies are evaluated theoreti-cally, using simulations and/or experimentally. Each transport technology is evaluated based on its performances and capabilities...... with respect to a num-ber of points. First, its capability to guarantee low delay and jitter during normal and high load conditions; this includes service model and reservation procedure. Secondly, how the technologies proactively and reactively handle failure situations in links and nodes including resource...

  13. Clinical Observation of Midazolam's Sedative Effect and Anterograde Amnesia Effect on Gynecologic Surgery Patients Undergoing Spinal-Epidural Anesthesia%咪唑安定联合腰硬联合麻醉对妇科手术患者镇静和术后遗忘效果观察

    Institute of Scientific and Technical Information of China (English)

    谢锐捷; 刘少芬; 张松林; 房瑞林; 陈铭君; 莫坚

    2013-01-01

    Objective:To explore the sedative effects and anterograde amnesia effect of midazolam intravenous injection used before combined spinal-epidural anesthesia on calming down the patients and their forgetting degree of procedure.Method:200 ASA Ⅰ-Ⅱpatients scheduled for gynecologic surgery under spinal-epidural anesthesia were included in the study.Patients were randomly assigned into two groups with 100 cases each.Group A used midazolam while Group B used 0.9% sodium as control.Group A used midazolam (0.lmg/kg) as intravenous injection 30 minutes before anesthesia while group B used 2ml 0.9% sodium as control.Record and compare the patients' sedative effect before operation began and the forgetting degree 24 hours after operation finished.Result:According to the Ramsay standard,the patients in group A have significant better sedative effect than group B since operation started (P<0.05).In group A the transient global amnesia rate of was 97% while the transient partial amnesia rate was 3%.Meanwhile,the transient global amnesia rate,the transient partial amnesia rate and the unforgotten rate were 68,25% and 7% in Group B separately.There was a significant difference between two groups (P<0.05).Conclusions:Midazolam (0.1mg/kg) as intravenous injection 30 minutes before anesthesia can reduce the patients' worries and horrors.It not only helps to keep patients sedative but also cooperate with anesthesia and controlling body position.It has good calm and amnesia effects on anesthesia and procedure.%目的:本文旨在观察咪唑安定联合腰硬联合麻醉对妇科手术患者术后镇痛遗忘效果.方法:将200例ASA Ⅰ~Ⅱ级腰硬联合麻醉下行妇科手术患者随机分为两组:咪唑安定组(A组)和对照组(B组).两组患者分别于麻醉前30分钟静脉注射咪唑安定0.1 mg/kg和0.9%生理盐水2mL.观察用药前后病人镇静情况,镇静分级,对麻醉和手术操作的遗忘程度和病人的心理状态.结果:根

  14. Hydrogen transport and storage in engineered microspheres

    Energy Technology Data Exchange (ETDEWEB)

    Rambach, G. [Lawrence Livermore National Lab., CA (United States); Hendricks, C. [W.J. Schafer Associates, Livermore, CA (United States)

    1996-10-01

    This project is a collaboration between Lawrence Livermore National Laboratory (LLNL) and W.J. Schafer Associates (WJSA). The authors plan to experimentally verify the performance characteristics of engineered glass microspheres that are relevant to the storage and transport of hydrogen for energy applications. They will identify the specific advantages of hydrogen transport by microspheres, analyze the infrastructure implications and requirements, and experimentally measure their performance characteristics in realistic, bulk storage situations.

  15. The German Saenger space transportation system concept

    Science.gov (United States)

    Koelle, D. E.; Kuczera, H.; Hoegenauer, E.

    The vehicle configuration, performance criteria, and technological problems of the Saenger space transportation system are reviewed. The vehicle consists of a two-stage system, including a hypersonic first stage employing turboramjet propulsion. The cruise speed is Mach 4.4, with the capability to accelerate to Mach 6.8 prior to separation of the upper stage. Two different upper stages are proposed to fulfull the different requirements of manned space flight and unmanned payload transportation.

  16. Magnetic method for stimulating transport in fluids

    Energy Technology Data Exchange (ETDEWEB)

    Martin, James E.; Solis, Kyle J.

    2016-10-18

    A method for producing mass and heat transport in fluids, wherein the method does not rely on conventional convection, that is, it does not require gravity, a thermal gradient, or a magnetic field gradient. This method gives rise to a unique class of vigorous, field-controllable flow patterns termed advection lattices. The advection lattices can be used to transport heat and/or mass in any desired direction using only magnetic fields.

  17. Simulated Ethanol Transportation Patterns and Costs

    OpenAIRE

    Thompson, Wyatt; Seth D. Meyer

    2009-01-01

    Ethanol production booms in the Midwest in 2007. Regulations require ethanol be included as a fuel additive in many areas as of 2006, though consumer willingness to adopt ethanol blends voluntarily is uncertain and benchmark ethanol and oil prices fluctuate. In this context, we jointly simulate consumer demand for ethanol and ethanol transportation costs. Results demonstrate a non-linear relationship between benchmark prices and transportation costs that depends critically on (1) the prevalen...

  18. Design evaluaion: pneumatic transport and classification

    International Nuclear Information System (INIS)

    This report describes the evaluation of selected design features of the cold engineering scale pneumatic transport and classification subsystems used in the development of the head-end equipment for HTGR fuel reprocessing. The report identifies areas that require further design effort and evaluation of alternatives prior to the design of the HTGR reference recycle facility (HRRF). Seven areas in the transport subsystem and three in the classification subsystem were selected for evaluation. Seventeen specific recommendations are presented for further design effort

  19. DNA charge transport: Moving beyond 1D

    Science.gov (United States)

    Zhang, Yuqi; Zhang, William B.; Liu, Chaoren; Zhang, Peng; Balaeff, Alexander; Beratan, David N.

    2016-10-01

    Charge transport across novel DNA junctions has been studied for several decades. From early attempts to move charge across DNA double crossover junctions to recent studies on DNA three-way junctions and G4 motifs, it is becoming clear that efficient cross-junction charge migration requires strong base-to-base electronic coupling at the junction, facilitated by favorable pi-stacking. We review recent progress toward the goal of manipulating and controlling charge transport through DNA junctions.

  20. Transportation of Hazardous Evidentiary Material.

    Energy Technology Data Exchange (ETDEWEB)

    Osborn, Douglas.

    2005-06-01

    This document describes the specimen and transportation containers currently available for use with hazardous and infectious materials. A detailed comparison of advantages, disadvantages, and costs of the different technologies is included. Short- and long-term recommendations are also provided.3 DraftDraftDraftExecutive SummaryThe Federal Bureau of Investigation's Hazardous Materials Response Unit currently has hazardous material transport containers for shipping 1-quart paint cans and small amounts of contaminated forensic evidence, but the containers may not be able to maintain their integrity under accident conditions or for some types of hazardous materials. This report provides guidance and recommendations on the availability of packages for the safe and secure transport of evidence consisting of or contaminated with hazardous chemicals or infectious materials. Only non-bulk containers were considered because these are appropriate for transport on small aircraft. This report will addresses packaging and transportation concerns for Hazardous Classes 3, 4, 5, 6, 8, and 9 materials. If the evidence is known or suspected of belonging to one of these Hazardous Classes, it must be packaged in accordance with the provisions of 49 CFR Part 173. The anthrax scare of several years ago, and less well publicized incidents involving unknown and uncharacterized substances, has required that suspicious substances be sent to appropriate analytical laboratories for analysis and characterization. Transportation of potentially hazardous or infectious material to an appropriate analytical laboratory requires transport containers that maintain both the biological and chemical integrity of the substance in question. As a rule, only relatively small quantities will be available for analysis. Appropriate transportation packaging is needed that will maintain the integrity of the substance, will not allow biological alteration, will not react chemically with the substance being

  1. Maritime transport operation in conditions of globalization

    Directory of Open Access Journals (Sweden)

    Tomasz Nowosielski

    2011-06-01

    Full Text Available The contemporary world economy is subject to dynamic integration processes that cause the most open countries in the free import and export goods. Intensive trade relations requires intercontinental free access to well-functioning transport systems. In the case of trade with distant partners, especially on other continents, an important criterion for the organization of cargo handling is a long range. Technological development of means of transport has meant that transport services have become a cheap and highly accessible to passengers and cargo.

  2. CRITICAL UNIT STREAM POWER FOR SEDIMENT TRANSPORT

    Institute of Scientific and Technical Information of China (English)

    2003-01-01

    Yang's (1996) sediment transport theory based on unit stream power is one of the most accurate theories, but in his equations the use of product of slope and critical velocity instead for critical unit stream power is not suitable. Dimensionless critical unit stream power required at incipient motion can be derived from the principle of conservation of power as a function of dimensionless particle diameter and relative roughness. Based on a lot of data sets, this new criterion was developed. By use of this new criteria, Yang's (1973) sand transport formula and his 1984 gravel transport formula could be improved when sediment concentration is less than about 100 ppm by weight.

  3. Transport regulations for radioactive material in Germany

    International Nuclear Information System (INIS)

    The transport of radioactive material in Germany is regulated by the dangerous goods transport regulations and the regulations of the Atomic Energy Act and the Radiation Protection Ordinance. For radioactive material shipments by road, rail, sea and air the modal regulations on the transport of dangerous goods for class 7 of the ADR, RID, ADNR, IMO and ICAO are implemented in Germany and have to be complied with. In parallel with this the Atomic Energy Act requirements and the provisions of the Radiation Protection Ordinance concerning the transport of radioactive material have to be met. They contain provisions regarding the reliability of transport organisations and persons, training of persons involved in transport nuclear liability insurance, physical protection and public interest in addition to the need to fulfil the dangerous goods transport regulations. According to these requirements shipment approvals which are presented in the paper, are necessary for nuclear material, large sources and other radioactive materials. Based on this some practical implications for radioactive material shipments will be discussed as well as some aspects of the future developments. The paper also gives an overview of the responsibilities for approval and inspection of radioactive material shipments in Germany. (author)

  4. TRANSPORT/HANDLING REQUESTS

    CERN Multimedia

    Groupe ST/HM

    2002-01-01

    A new EDH document entitled 'Transport/Handling Request' will be in operation as of Monday, 11th February 2002, when the corresponding icon will be accessible from the EDH desktop, together with the application instructions. This EDH form will replace the paper-format transport/handling request form for all activities involving the transport of equipment and materials. However, the paper form will still be used for all vehicle-hire requests. The introduction of the EDH transport/handling request form is accompanied by the establishment of the following time limits for the various services concerned: 24 hours for the removal of office items, 48 hours for the transport of heavy items (of up to 6 metric tons and of standard road width), 5 working days for a crane operation, extra-heavy transport operation or complete removal, 5 working days for all transport operations relating to LHC installation. ST/HM Group, Logistics Section Tel: 72672 - 72202

  5. Transportation activities for BWR fuels at NFI

    International Nuclear Information System (INIS)

    Nuclear Fuel Industries, LTD. (NFI) supplies fuel assemblies for both PWR and BWR in Japan. We can also manage transportation of the fuel assemblies from our fabrication facilities to the nuclear power plants of Japanese utilities. For the transportation of fuel assembly, we designed and fabricated the transportation containers to meet the requirements of the IAEA regulations, and licensed in Japan. This paper introduced the recent activity and R and D of NFI concerning transportation of BWR fuel assembly. NT-XII transportation container was developed for fresh BWR fuel assemblies. NT-XII container consists of inner container and outer container. Two BWR fuel assemblies with up to 5 wt.-% 235-U enriched are enclosed in an inner container. In the concept of NT-XII container design, we made the best priority to transportation efficiency, as well as ensuring fuel integrity during transportation. NT-XII has been used since 2002 in Japan. Thanks to the lightening weight of containers, the number of containers to be loaded to one transportation truck was increased up to 9 containers (equivalent to 18 fuel assemblies) compared with former type container (NT-IV transportation container) which can be loaded up to 6 containers (equivalent to 12 fuel assemblies). In addition to the design of brand-new container, we promote the improvement of the packaging methods. In Japan, in order to reduce the damage to the fuel rod and fuel spacer while transporting, polyethylene sleeves which are called 'packing separators' are inserted in the rod-to-rod gap of fuel assembly. However, packing separators requires time and cost for the installation at fuel fabrication facility. In the same way, huge time and cost are needed for removal of packing separators at nuclear power plant. For the improvement of preparation efficiency before and after transportation, we investigated the influence of vibration to fuel integrity in case of transportation without packing separators. Based on the above

  6. Quantification of risks at the transport of radioactive materials

    International Nuclear Information System (INIS)

    Requirement of the risks which are coherent with the transport of hazardous elements are for the time being drawn up in a joint project by the Netherlands Ministries of SZW and VROM. In this project 'Requirements of risk for the transport of hazardous elements' (RNVGS) the transport of radioactive elements is not considered. To reach requirements for such a transport, the Directorate of Elements, Safety and Radiation of VROM has provided an assignment to ECN to make a quantitative analysis on the risks of transport of radioactive elements on the road. At the same time, they requested to examine in this performance study whether there are needed additional criteria specific for transport, along the criteria used for individual risk and group-risks. 55 refs., 1 fig., 16 refs

  7. US cask requirements and industry capability survey

    International Nuclear Information System (INIS)

    The objectives of this paper are to provide an estimate of spent fuel shipping cask requirements for reactor to away-from-reactor (AFR) storage facility shipments from the present time until late in this century and to determine and document the willingness and capability of private industry to provide required future transportation services. In order to meet this objective, the Transportation Technology Center at Sandia National Laboratories sponsored Teledyne Energy Systems to conduct a survey of US industry. Results of tasks completed to carry out the objectives are reviewed

  8. The transportation of hazardous materials

    International Nuclear Information System (INIS)

    The increasing use of dangerous chemicals and petroleum products by S.A. industry makes it necessary for some form of control to be introduced to regulate the transport of these materials before a major disaster occurs, such as has occurred overseas. This report examines all the aspects that could increase the likelihood of such a disaster occurring, including the preparedness of emergency services. It also recommends the improvements or changes required to minimize this possibility. It is apparent that the training and ability of vehicle drivers are key areas in this respect and they are discussed at length. Forthcoming regulations under the Hazardous Substances Act No. 15 of 1973 are examined and the effects of over-restrictive legislation considered. The report concludes that legislation promulgated gradually to reinforce voluntary industrial practices will ultimately restrict this type of transport to the safety-conscious and competent operator, therefore minimizing the risk as much as possible

  9. Product through pipelines : transportation options

    Energy Technology Data Exchange (ETDEWEB)

    Ballyntyne, R. [Terasen Pipelines, Calgary, AB (Canada)

    2004-07-01

    This PowerPoint presentation provided a brief overview of the corporate structure of Terasen Pipelines along with a map displaying the locations of the pipelines it operates and new directions for growth. It also presented a proposal to deliver petroleum products from Alberta's oilsand fields to markets via the Corridor and Express pipeline systems, and evaluated the issue of transporting petrochemicals via rail transport rather than pipeline. Industry is also examining the option of shipping refined products through a trans mountain system from Alberta to British Columbia and California. A chart highlighting Canadian crude oil production was presented along with an oil sands pipeline capacity chart. It was noted that significant investment in refining capacity is needed and that new pipeline facilities will be required to meet the needs of oil sands developments. tabs., figs.

  10. L’AUTONOMIE DU CONTRAT DE TRANSPORT

    Directory of Open Access Journals (Sweden)

    Ana CĂLIN

    2006-01-01

    Full Text Available The autonomy of the transport contract was developed over time having as basis few elements of legislation. Practice was the one that, in need of moving goods and persons, outlined the elements that are the basis of the transport contract. It was said that it is a civil or a commercial contract, which is distinguished by the quality of trader or non-trader of the carter. The essential element that distinguishes it from other contracts is that during the execution of the transport contract there are involved three persons, namely: the consignor, the carter and the consignee.The legal form and the autonomy of the transport contract result from the fact that there is a real and a consensual contract, that has an economic content in different ways; the parties of the transport contract must understand that the general conditions on the ability to control the consent lawfully expressed, has a determined object, licit and moral feature and the form required by law. It is a commutative contract. The transport contract is concluded in written form. E. Cristoforeanu, Constantin Stătescu, Cezare Vivante investigated about the autonomy’s development and stability of the transport contract. Such distinguished personalities expressed their opinions regarding the definition of transport contract as an autonomous contract, even though it borrowed elements of civil law and commercial law.

  11. QUALITATIVE OPERATION INDICES OF TRANSPORT COMPLEX OF THE RUSSIAN FEDERATION

    Directory of Open Access Journals (Sweden)

    I. N. Pugachev

    2015-01-01

    Full Text Available The paper considers existing qualitative indices of transport services. Indices for evaluation of qualitative operation indices of transport complex have been proposed in the paper. Special attention has been devoted to interaction and coordination of various types of transport as the highest level of qualitative operation of transport complexes. Operational coordination of transport facilities is considered as introduction of modern management, information and communication systems while using satellite navigation-information systems. The paper has studied the possibility to create a modern automated management system for transport complex at the Ministry of Transport of the Russian Federation that ensures an efficient organization of standardized information exchange between transport complex participants.The paper provides efficient solutions on transport problems that lead to planned improvements of objective indices with small expenses. A special focus has been given to development of transport systems in large cities with the purpose to reveal their impact on operation of the national transport complex. The paper considers conceptual moments pertaining to designing and realization of an intellectual management system and creation of complex conditions that ensure maximally efficient transport management on city territory.The main purpose of transport infrastructure modernization in accordance with its types is to create modern efficient transport facilities and equipment for provision of technological infrastructure required for the system of objects included in the transport infrastructure. The modernization strategy must be governed by national policy in the field of any transport and in accordance with international development tendencies in transport industry and transport construction. In this respect the transport infrastructure is the very first consumer of innovative high technologies that determine scientific

  12. Transport of radioactive substances; Der Transport radioaktiver Stoffe

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2014-12-15

    The report on the transport of radioactive substances covers the following topics: facts on radioactive materials transport, safety of the transport of radioactive substances, legal regulations and guidelines: a multiform but consistent system, transport of nuclear fuels, safety during the transport of nuclear fuel, future transport of spent fuel elements and high-level radioactive wastes in Germany.

  13. 49 CFR 192.939 - What are the required reassessment intervals?

    Science.gov (United States)

    2010-10-01

    ... SAFETY TRANSPORTATION OF NATURAL AND OTHER GAS BY PIPELINE: MINIMUM FEDERAL SAFETY STANDARDS Gas... 49 Transportation 3 2010-10-01 2010-10-01 false What are the required reassessment intervals? 192.939 Section 192.939 Transportation Other Regulations Relating to Transportation (Continued)...

  14. Groupware requirements evolution patterns

    NARCIS (Netherlands)

    Pumareja, Dulce Trinidad

    2013-01-01

    Requirements evolution is a generally known problem in software development. Requirements are known to change all throughout a system's lifecycle. Nevertheless, requirements evolution is a poorly understood phenomenon. Most studies on requirements evolution focus on changes to written specifications

  15. High capacity carrier ethernet transport networks

    DEFF Research Database (Denmark)

    Rasmussen, Anders; Zhang, Jiang; Yu, Hao;

    2009-01-01

    Ethernet as a transport technology has, up to now, lacked the features such as network layer architecture, customer separation and manageability that carriers require for wide-scale deployment. However, with the advent of PBB-TE and T-MPLS, it is now possible to use Ethernet as a transport...... OAM functions, survivability and the increased bandwidth requirements of carrier class systems. This article provides an overview of PBB-TE and T-MPLS and demonstrates how IPTV services can be realized in the framework of Carrier Ethernet. In addition we provide a case study on performing bit error...

  16. Transport, energy and environment

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-12-01

    Transportation demands a large and increasing share of total energy consumption in Europe. At the same time many European countries are facing difficult decisions in achieving their long term environmental goals. Therefore energy policy, environmental policy and transport policy should be seen and discussed in a common perspective. In particular the relative contribution from the transport sector and the energy sector involves a number of important and difficult issues. The aim of the conference was to bring together economists, scientists, manufactures, energy planners, transport planners, and decision makers in order to discuss the importance of the transport sector in relation to energy demand and long term environmental goals. General conference sessions covered. Trends in Transport Energy Demand and Environmental constraints, Technological Development and New Transport Systems, Lifestyle Changes and the Transport Sector, Megacities: Solutions to the Transport and Air Pollution Problems, Effectiveness of Public Policies, Transport and Energy sector, and Methods, Models and Data. The conference took place at Hotel Marienlyst, Elsinore, Denmark and attracted wide interest. The participants represented 14 different countries covering international organisations, ministries, universities, research centres, consulting firms, industry etc. (EG)

  17. Transport Properties for Combustion Modeling

    Energy Technology Data Exchange (ETDEWEB)

    Brown, N.J.; Bastein, L.; Price, P.N.

    2010-02-19

    This review examines current approximations and approaches that underlie the evaluation of transport properties for combustion modeling applications. Discussed in the review are: the intermolecular potential and its descriptive molecular parameters; various approaches to evaluating collision integrals; supporting data required for the evaluation of transport properties; commonly used computer programs for predicting transport properties; the quality of experimental measurements and their importance for validating or rejecting approximations to property estimation; the interpretation of corresponding states; combination rules that yield pair molecular potential parameters for unlike species from like species parameters; and mixture approximations. The insensitivity of transport properties to intermolecular forces is noted, especially the non-uniqueness of the supporting potential parameters. Viscosity experiments of pure substances and binary mixtures measured post 1970 are used to evaluate a number of approximations; the intermediate temperature range 1 < T* < 10, where T* is kT/{var_epsilon}, is emphasized since this is where rich data sets are available. When suitable potential parameters are used, errors in transport property predictions for pure substances and binary mixtures are less than 5 %, when they are calculated using the approaches of Kee et al.; Mason, Kestin, and Uribe; Paul and Warnatz; or Ern and Giovangigli. Recommendations stemming from the review include (1) revisiting the supporting data required by the various computational approaches, and updating the data sets with accurate potential parameters, dipole moments, and polarizabilities; (2) characterizing the range of parameter space over which the fit to experimental data is good, rather than the current practice of reporting only the parameter set that best fits the data; (3) looking for improved combining rules, since existing rules were found to under-predict the viscosity in most cases; (4

  18. 49 CFR 37.27 - Transportation for elementary and secondary education systems.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 1 2010-10-01 2010-10-01 false Transportation for elementary and secondary education systems. 37.27 Section 37.27 Transportation Office of the Secretary of Transportation... elementary and secondary education systems. (a) The requirements of this part do not apply to public...

  19. 49 CFR 176.174 - Transport of Class 1 (explosive) materials in shipborne barges.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 2 2010-10-01 2010-10-01 false Transport of Class 1 (explosive) materials in... REGULATIONS CARRIAGE BY VESSEL Detailed Requirements for Class 1 (Explosive) Materials Cargo Transport Units and Shipborne Barges § 176.174 Transport of Class 1 (explosive) materials in shipborne barges....

  20. 14 CFR 91.605 - Transport category civil airplane weight limitations.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 2 2010-01-01 2010-01-01 false Transport category civil airplane weight... Equipment and Operating Requirements for Large and Transport Category Aircraft § 91.605 Transport category civil airplane weight limitations. (a) No person may take off any transport category airplane...

  1. 49 CFR 176.170 - Transport of Class 1 (explosive) materials in freight containers.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 2 2010-10-01 2010-10-01 false Transport of Class 1 (explosive) materials in... REGULATIONS CARRIAGE BY VESSEL Detailed Requirements for Class 1 (Explosive) Materials Cargo Transport Units and Shipborne Barges § 176.170 Transport of Class 1 (explosive) materials in freight containers....

  2. 49 CFR 174.61 - Transport vehicles and freight containers on flat cars.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 2 2010-10-01 2010-10-01 false Transport vehicles and freight containers on flat... CARRIAGE BY RAIL General Handling and Loading Requirements § 174.61 Transport vehicles and freight containers on flat cars. (a) A transport vehicle, freight container, or package containing a...

  3. 49 CFR 176.168 - Transport of Class 1 (explosive) materials in vehicle spaces.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 2 2010-10-01 2010-10-01 false Transport of Class 1 (explosive) materials in... REGULATIONS CARRIAGE BY VESSEL Detailed Requirements for Class 1 (Explosive) Materials Cargo Transport Units and Shipborne Barges § 176.168 Transport of Class 1 (explosive) materials in vehicle spaces. (a)...

  4. An ATP transport system in the intracellular bacterium, Bdellovibrio bacteriovorus 109J.

    OpenAIRE

    Ruby, E G; McCabe, J B

    1986-01-01

    The intracellularly growing bacterium Bdellovibrio bacteriovorus 109J transports intact ATP by a specific, energy-requiring process. ATP transport does not involve either an ADP-ATP or an AMP-ATP exchange mechanism but, instead, has characteristics of an active transport permease. Kinetically distinct systems for ATP transport are expressed by the two developmental stages of the bdellovibrio life cycle.

  5. Feed tank transfer requirements

    International Nuclear Information System (INIS)

    This document presents a definition of tank turnover; DOE responsibilities; TWRS DST permitting requirements; TWRS Authorization Basis (AB) requirements; TWRS AP Tank Farm operational requirements; unreviewed safety question (USQ) requirements; records and reporting requirements, and documentation which will require revision in support of transferring a DST in AP Tank Farm to a privatization contractor for use during Phase 1B

  6. Hazardous Material Packaging and Transportation

    Energy Technology Data Exchange (ETDEWEB)

    Hypes, Philip A. [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

    2016-02-04

    This is a student training course. Some course objectives are to: recognize and use standard international and US customary units to describe activities and exposure rates associated with radioactive material; determine whether a quantity of a single radionuclide meets the definition of a class 7 (radioactive) material; determine, for a given single radionuclide, the shipping quantity activity limits per 49 Code of Federal Regulations (CFR) 173.435; determine the appropriate radioactive material hazard class proper shipping name for a given material; determine when a single radionuclide meets the DOT definition of a hazardous substance; determine the appropriate packaging required for a given radioactive material; identify the markings to be placed on a package of radioactive material; determine the label(s) to apply to a given radioactive material package; identify the entry requirements for radioactive material labels; determine the proper placement for radioactive material label(s); identify the shipping paper entry requirements for radioactive material; select the appropriate placards for a given radioactive material shipment or vehicle load; and identify allowable transport limits and unacceptable transport conditions for radioactive material.

  7. Yeast Interacting Proteins Database: YNR051C, YER151C [Yeast Interacting Proteins Database

    Lifescience Database Archive (English)

    Full Text Available that coregulates anterograde and retrograde transport between the endoplasmic reticulum and Golgi compartme...C UBP3 Ubiquitin-specific protease that interacts with Bre5p to co-regulate anterograde and retrograde...t gene name BRE5 Bait description Ubiquitin protease cofactor, forms deubiquitination complex with Ubp3p that coregulates anterograde... and retrograde transport between the endoplasmic reticulum and Golgi compartments;...3 Prey description Ubiquitin-specific protease that interacts with Bre5p to co-regulate anterograde and retrograde

  8. ISSUES ASSOCIATED WITH SAFE PACKAGING AND TRANSPORT OF NANOPARTICLES

    Energy Technology Data Exchange (ETDEWEB)

    Gupta, N.; Smith, A.

    2011-02-14

    Nanoparticles have long been recognized a hazardous substances by personnel working in the field. They are not, however, listed as a separate, distinct category of dangerous goods at present. As dangerous goods or hazardous substances, they require packaging and transportation practices which parallel the established practices for hazardous materials transport. Pending establishment of a distinct category for such materials by the Department of Transportation, existing consensus or industrial protocols must be followed. Action by DOT to establish appropriate packaging and transport requirements is recommended.

  9. Nursing staff requirements for neonatal intensive care.

    OpenAIRE

    Williams, S.; Whelan, A; Weindling, A M; Cooke, R W

    1993-01-01

    A study to estimate the number of nursing staff required for neonatal nursing was undertaken. Certain nursing tasks, such as transporting any infant, caring for the dying infant, and looking after the very unstable infant required continuous attention by one nurse (5.5 whole time equivalent (wte) nurses for each cot). The stable ventilated infant required 10.5 nursing hours each day-that is, 2.4 wte/cot. Infants with intravenous infusions, but not ventilated, required only slightly less nursi...

  10. EBS Radionuclide Transport Abstraction

    International Nuclear Information System (INIS)

    The purpose of this report is to develop and analyze the engineered barrier system (EBS) radionuclide transport abstraction model, consistent with Level I and Level II model validation, as identified in Technical Work Plan for: Near-Field Environment and Transport: Engineered Barrier System: Radionuclide Transport Abstraction Model Report Integration (BSC 2005 [DIRS 173617]). The EBS radionuclide transport abstraction (or EBS RT Abstraction) is the conceptual model used in the total system performance assessment (TSPA) to determine the rate of radionuclide releases from the EBS to the unsaturated zone (UZ). The EBS RT Abstraction conceptual model consists of two main components: a flow model and a transport model. Both models are developed mathematically from first principles in order to show explicitly what assumptions, simplifications, and approximations are incorporated into the models used in the TSPA. The flow model defines the pathways for water flow in the EBS and specifies how the flow rate is computed in each pathway. Input to this model includes the seepage flux into a drift. The seepage flux is potentially split by the drip shield, with some (or all) of the flux being diverted by the drip shield and some passing through breaches in the drip shield that might result from corrosion or seismic damage. The flux through drip shield breaches is potentially split by the waste package, with some (or all) of the flux being diverted by the waste package and some passing through waste package breaches that might result from corrosion or seismic damage. Neither the drip shield nor the waste package survives an igneous intrusion, so the flux splitting submodel is not used in the igneous scenario class. The flow model is validated in an independent model validation technical review. The drip shield and waste package flux splitting algorithms are developed and validated using experimental data. The transport model considers advective transport and diffusive transport

  11. EBS Radionuclide Transport Abstraction

    Energy Technology Data Exchange (ETDEWEB)

    J. Prouty

    2006-07-14

    The purpose of this report is to develop and analyze the engineered barrier system (EBS) radionuclide transport abstraction model, consistent with Level I and Level II model validation, as identified in Technical Work Plan for: Near-Field Environment and Transport: Engineered Barrier System: Radionuclide Transport Abstraction Model Report Integration (BSC 2005 [DIRS 173617]). The EBS radionuclide transport abstraction (or EBS RT Abstraction) is the conceptual model used in the total system performance assessment (TSPA) to determine the rate of radionuclide releases from the EBS to the unsaturated zone (UZ). The EBS RT Abstraction conceptual model consists of two main components: a flow model and a transport model. Both models are developed mathematically from first principles in order to show explicitly what assumptions, simplifications, and approximations are incorporated into the models used in the TSPA. The flow model defines the pathways for water flow in the EBS and specifies how the flow rate is computed in each pathway. Input to this model includes the seepage flux into a drift. The seepage flux is potentially split by the drip shield, with some (or all) of the flux being diverted by the drip shield and some passing through breaches in the drip shield that might result from corrosion or seismic damage. The flux through drip shield breaches is potentially split by the waste package, with some (or all) of the flux being diverted by the waste package and some passing through waste package breaches that might result from corrosion or seismic damage. Neither the drip shield nor the waste package survives an igneous intrusion, so the flux splitting submodel is not used in the igneous scenario class. The flow model is validated in an independent model validation technical review. The drip shield and waste package flux splitting algorithms are developed and validated using experimental data. The transport model considers advective transport and diffusive transport

  12. Transport Energy Efficiency

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2010-07-01

    Transport is the sector with the highest final energy consumption and, without any significant policy changes, is forecast to remain so. In 2008, the IEA published 25 energy efficiency recommendations, among which four are for the transport sector. The recommendations focus on road transport and include policies on improving tyre energy efficiency, fuel economy standards for both light-duty vehicles and heavy-duty vehicles, and eco-driving. Implementation of the recommendations has been weaker in the transport sector than others. This paper updates the progress that has been made in implementing the transport energy efficiency recommendations in IEA countries since March 2009. Many countries have in the last year moved from 'planning to implement' to 'implementation underway', but none have fully implemented all transport energy efficiency recommendations. The IEA calls therefore for full and immediate implementation of the recommendations.

  13. Liver iron transport

    Institute of Scientific and Technical Information of China (English)

    Ross M Graham; Anita CG Chua; Carly E Herbison; John K Olynyk; Debbie Trinder

    2007-01-01

    The liver plays a central role in iron metabolism. It is the major storage site for iron and also expresses a complex range of molecules which are involved in iron transport and regulation of iron homeostasis. An increasing number of genes associated with hepatic iron transport or regulation have been identified. These include transferrin receptors (TFR1 and 2), a ferrireductase (STEAP3), the transporters divalent metal transporter-1 (DMT1) and ferroportin (FPN) as well as the haemochromatosis protein, HFE and haemojuvelin (HJV),which are signalling molecules. Many of these genes also participate in iron regulatory pathways which focus on the hepatic peptide hepcidin. However, we are still only beginning to understand the complex interactions between liver iron transport and iron homeostasis. This review outlines our current knowledge of molecules of iron metabolism and their roles in iron transport and regulation of iron homeostasis.

  14. Means of Transport

    DEFF Research Database (Denmark)

    Balle, Søren Hattesen

    2004-01-01

    improved means of fast physical movement from one place to another. Yet, the impact that the technology of automobility came to have was not of a limited materialistic nature. As many literary and cultural critics have noted, car technology extended its influence into the field of imaginative transport....... According to film theorist Julian Smith, the automobile has been just as much “embraced … as a form of emotional transport, the state or condition of being transported by ecstasy” as “perceived as a mode of transportation in the primary and ordinary sense of the word.” This paper analyses the ways in which...... William Carlos Williams negotiates the relationship between these two kinds of automotive transport in some of his poetic and poetic prose writings from the period when the car was introduced on a more general scale in America. Intense moments of emotional and imaginative transport in Williams are often...

  15. Transport of radioactive material at IPEN-CNEN/SP

    International Nuclear Information System (INIS)

    Transport plays an important role in the worldwide nuclear industry and the safety record of nuclear transport across the world is excellent. This is due to well-grounded regulations developed by governmental and intergovernmental organizations and to the professional ability of those in the industry. This paper presents an overview of the activities related to the transport of radioactive material at IPEN-CNEN/SP. In particular, the applicable legislation, the responsibilities and tasks of the competent authorities are discussed. The categories of radioactive materials transported and the packaging requirements for the safe transport of these radioactive materials are also described.(author)

  16. Human iron transporters

    OpenAIRE

    Garrick, Michael D.

    2010-01-01

    Human iron transporters manage iron carefully because tissues need iron for critical functions, but too much iron increases the risk of reactive oxygen species. Iron acquisition occurs in the duodenum via divalent metal transporter (DMT1) and ferroportin. Iron trafficking depends largely on the transferrin cycle. Nevertheless, non-digestive tissues have a variety of other iron transporters that may render DMT1 modestly redundant, and DMT1 levels exceed those needed for the just-mentioned task...

  17. Sustainability and Transport

    OpenAIRE

    Gilbert, Richard

    2006-01-01

    Almost 20 years after the term “sustainable development” was popularized in the Brundtland Report (World Commission on Environment and Development 1987), the U.S. government turned its attention towards application of the concept of sustainability to transport planning. In response to a 2003 request by the Secretary of Transportation, the Transportation Research Board (TRB) of the National Research Council established a committee to consider how sustainability could be integrated into transpo...

  18. Transport properties of organic liquids

    CERN Document Server

    Latini, G; Passerini, G

    2006-01-01

    The liquid state is possibly the most difficult and intriguing state of matter to model. Organic liquids are required, mainly as working fluids, in almost all industrial activities and in most appliances (e.g. in air conditioning). Transport properties (namely dynamic viscosity and thermal conductivity) are possibly the most important properties for the design of devices and appliances. The aim of this book is to present both theoretical approaches and the latest experimental advances on the issue, and to merge them into a wider approach. It concentrates on applicability of models.This book is

  19. Intelligent Freigth Transport Systems

    DEFF Research Database (Denmark)

    Overø, Helene Martine; Larsen, Allan; Røpke, Stefan

    2009-01-01

    The Danish innovation project entitled “Intelligent Freight Transport Systems” aims at developing prototype systems integrating public intelligent transport systems (ITS) with the technology in vehicles and equipment as well as the IT-systems at various transport companies. The objective...... is to enhance the efficiency and lower the environmental impact in freight transport. In this paper, a pilot project involving real-time waste collection at a Danish waste collection company is described, and a solution approach is proposed. The problem corresponds to the dynamic version of the waste collection...

  20. RELATIVISTIC TRANSPORT-THEORY

    NARCIS (Netherlands)

    MALFLIET, R

    1993-01-01

    We discuss the present status of relativistic transport theory. Special emphasis is put on problems of topical interest: hadronic features, thermodynamical consistent approximations and spectral properties.

  1. Thermodynamics of ABC transporters.

    Science.gov (United States)

    Zhang, Xuejun C; Han, Lei; Zhao, Yan

    2016-01-01

    ABC transporters form the largest of all transporter families, and their structural study has made tremendous progress over recent years. However, despite such advances, the precise mechanisms that determine the energy-coupling between ATP hydrolysis and the conformational changes following substrate binding remain to be elucidated. Here, we present our thermodynamic analysis for both ABC importers and exporters, and introduce the two new concepts of differential-binding energy and elastic conformational energy into the discussion. We hope that the structural analysis of ABC transporters will henceforth take thermodynamic aspects of transport mechanisms into account as well.

  2. WASTE PACKAGE TRANSPORTER DESIGN

    Energy Technology Data Exchange (ETDEWEB)

    D.C. Weddle; R. Novotny; J. Cron

    1998-09-23

    The purpose of this Design Analysis is to develop preliminary design of the waste package transporter used for waste package (WP) transport and related functions in the subsurface repository. This analysis refines the conceptual design that was started in Phase I of the Viability Assessment. This analysis supports the development of a reliable emplacement concept and a retrieval concept for license application design. The scope of this analysis includes the following activities: (1) Assess features of the transporter design and evaluate alternative design solutions for mechanical components. (2) Develop mechanical equipment details for the transporter. (3) Prepare a preliminary structural evaluation for the transporter. (4) Identify and recommend the equipment design for waste package transport and related functions. (5) Investigate transport equipment interface tolerances. This analysis supports the development of the waste package transporter for the transport, emplacement, and retrieval of packaged radioactive waste forms in the subsurface repository. Once the waste containers are closed and accepted, the packaged radioactive waste forms are termed waste packages (WP). This terminology was finalized as this analysis neared completion; therefore, the term disposal container is used in several references (i.e., the System Description Document (SDD)) (Ref. 5.6). In this analysis and the applicable reference documents, the term ''disposal container'' is synonymous with ''waste package''.

  3. Heavy transport problems

    International Nuclear Information System (INIS)

    Assuming that very often a long transport route from the factory of the manufacturer to the provided site has to be reckoned with, in general only transport with a ship is possible. As each site is only called by a certain steamship line, at a very early stage of planning the nuclear power plant the possibilities and capacities of the lines and means of transportation under discussion should be investigated. In planning the unloading equipment at the site, due consideration should be given to the fact that at a later time this equipment should also be suitable for the transport of heavy components and spent fuel assemblies. (orig.)

  4. EUROPEAN MARITIME TRANSPORT POLICY

    Directory of Open Access Journals (Sweden)

    Jerzy Kujawa

    2014-03-01

    Full Text Available The article describes the common EU policy on maritime transport, which comprises almost 80% of the volume of external trade of the Union and about 40% of internal transport needs. The first part of the paper presents the origins of the common maritime transport policy and the difficulties encountered during its initial formation. Subsequently, the evolution of the concepts of the policy and its current shape is discussed. The final, substantial part of the article describes the main aims and directions of the EU maritime transport policy and includes an evaluation of the effects of the policy.

  5. Moisture Transport in Wood

    DEFF Research Database (Denmark)

    Astrup, T.; Hansen, K. K.; Hoffmeyer, P.;

    2005-01-01

    Modelling of moisture transport in wood is of great importance as most mechanical and physical properties of wood depend on moisture content. Moisture transport in porous materials is often described by Ficks second law, but several observations indicate that this does not apply very well to wood....... Recently at the Technical University of Denmark, Department of Civil Engineering, a new model for moisture transport in wood has been developed. The model divides the transport into two phases, namely water vapour in the cell lumens and bound water in the cell walls....

  6. Hopping transport in solids

    CERN Document Server

    Pollak, M

    1991-01-01

    The hopping process, which differs substantially from conventional transport processes in crystals, is the central process in the transport phenomena discussed in this book. Throughout the book the term ``hopping'' is defined as the inelastic tunneling transfer of an electron between two localized electronic states centered at different locations. Such processes do not occur in conventional electronic transport in solids, since localized states are not compatible with the translational symmetry of crystals.The rapid growth of interest in hopping transport has followed in the footsteps of the

  7. UZ Colloid Transport Model

    Energy Technology Data Exchange (ETDEWEB)

    M. McGraw

    2000-04-13

    The UZ Colloid Transport model development plan states that the objective of this Analysis/Model Report (AMR) is to document the development of a model for simulating unsaturated colloid transport. This objective includes the following: (1) use of a process level model to evaluate the potential mechanisms for colloid transport at Yucca Mountain; (2) Provide ranges of parameters for significant colloid transport processes to Performance Assessment (PA) for the unsaturated zone (UZ); (3) Provide a basis for development of an abstracted model for use in PA calculations.

  8. Alternatives to traditional transportation fuels: An overview

    Energy Technology Data Exchange (ETDEWEB)

    1994-06-01

    This report presents the first compilation by the Energy Information Administration (EIA) of information on alternatives to gasoline and diesel fuel. The purpose of the report is: (1) to provide background information on alternative transportation fuels and replacement fuels compared with gasoline and diesel fuel, and (2) to furnish preliminary estimates of alternative transportation fuels and alternative fueled vehicles as required by the Energy Policy Act of 1992 (EPACT), Title V, Section 503, ``Replacement Fuel Demand Estimates and Supply Information.`` Specifically, Section 503 requires the EIA to report annually on: (1) the number and type of alternative fueled vehicles in existence the previous year and expected to be in use the following year, (2) the geographic distribution of these vehicles, (3) the amounts and types of replacement fuels consumed, and (4) the greenhouse gas emissions likely to result from replacement fuel use. Alternative fueled vehicles are defined in this report as motorized vehicles licensed for on-road use, which may consume alternative transportation fuels. (Alternative fueled vehicles may use either an alternative transportation fuel or a replacement fuel.) The intended audience for the first section of this report includes the Secretary of Energy, the Congress, Federal and State agencies, the automobile manufacturing industry, the transportation fuel manufacturing and distribution industries, and the general public. The second section is designed primarily for persons desiring a more technical explanation of and background for the issues surrounding alternative transportation fuels.

  9. Final beam transport in the reactor chamber

    International Nuclear Information System (INIS)

    The beam transport in heavy ion fusion (HIF) accelerators is discussed. The qualitative features of transport effects are presented. The basic transport effects associated with HIF beam are space charge effects, atomic physics effects, zero-order plasma effects, and plasma instabilities. In the case of HIF, very high intensity of HIF beam is required, and its own electric repulsion does not keep the beam converging. The number of beams required for supplying the demand power at a target can be estimated. The beam charge deposited on a target pellet produces electrostatic potential, and the electrostatic repulsion prevents the beam to reach on the target. The upper limit of the gas pressure is determined by small angle Coulomb scattering. Since unneutralized beam has the pinching force, the electrostatic kink mode (wiggle mode) should be considered in the pressure region where beam neutralization does not occur. Two-stream instability, filamentation instability and self-pinched transport are considered. As a conclusion of this paper, the new first choice for HIF transport is to use ballistic transport in moderate vacuum. (Kato, T.)

  10. Low energy beam transport system developments

    Science.gov (United States)

    Dudnikov, V.; Han, B.; Stockli, M.; Welton, R.; Dudnikova, G.

    2015-04-01

    For high brightness beam production it is important to preserve the brightness in the low energy beam transport system (LEBT) used to transport and match the ion beams to the next stage of acceleration, usually an RFQ. While electrostatic focusing can be problematic for high current beam transport, reliable electrostatic LEBT operation has been demonstrated with H- beams up to 60 mA. Now, however, it is commonly accepted that an optimal LEBT for high current accelerator applications consists of focusing solenoids with space charge compensation. Two-solenoid LEBTs are successfully used for high current (>100 mA) proton beam transport. Preservation of low emittances (~0.15 π mm-mrad) requires the addition of a heavy gas (Xe, Kr), which causes ~5% of proton loss in a 1 m long LEBT. Similar Xe densities would be required to preserve low emittances of H- beams, but such gas densities cause unacceptably high H- beam losses. A short LEBT with only one short solenoid, movable for RFQ matching, can be used for reduced negative ion stripping. A strong electrostatic-focusing LEBT has been successfully adopted for transport of high current H- beams in the SNS Front End. Some modifications of such electrostatic LEBTs are expected to improve the reliable transport of intense positive and negative ion beams without greatly degrading their low emittances. We concentrate on processes that determine the beam brightness degradation and on their prevention. Proposed improvements to the SNS electrostatic LEBT are discussed.

  11. Rail transportation of biofuels; Jaernvaegstransport av biobraenslen

    Energy Technology Data Exchange (ETDEWEB)

    Frosch, Martin; Thoren, Peter

    2010-05-15

    The project has had the task of describing how the rail transportation of biofuels can be increased. This has been made by analyzing the current conditions of five transmission terminals and five different CHP plants, henceforth called reference cases, and the prevailing conditions at their reception terminals. For each of the five reference cases several transport solutions have been created that take in consideration how these conditions affect the efficiency of the transport solutions for 23 different relations. The study will also show to what extent reduction of environmental impacts can be achieved with the rail transportation of biofuels as alternative to road transport. Three of the reference cases are in operation, one under construction and one in the development phase. To develop transportation solutions and identify potential catchment areas for biofuels a slightly higher resolution than what normally is analyzed in existing literature is required. Therefore, these analysis have been made for each of the relevant catchment areas with the help of forest assessment data, kNN-data, from the Swedish agricultural university (SLU), statistics of peat production from the Swedish geological survey (SGU) and the Forestry boards (Skogsstyrelsen) statistical yearbook of 2008. The calculations underlying the transport solutions are relating to the sustainability of each catchment area, constraints and opportunities at both the reception and transmission terminals, and the practical solutions for wagons, containers and number of possible weekly circulations. The target audience is the heating and CHP plants in Sweden, wishing to use rail transport of biofuels, regardless of available infrastructure since remote receiving terminals also can be used if needed. The results of the economical analysis for all possible relations are based on calculations with 20 wagons in each circulation. Due to topographic limitations and limited traction capacity all relations

  12. Environmentally sustainable transport in the CEI countries

    International Nuclear Information System (INIS)

    Moving people and freight in an environmentally sustainable manner that reduces environmental pollution and health hazards is a key challenge for transport and environment policies in Europe. Present mobility patterns of passenger and freight transport in Central and Eastern Europe do not correspond to the objectives of sustainable development. This paper presents the results of a transport futures study for the CEI region as a whole using backcasting methodology with long-term sustainability criteria to be met by 2030. Achieving environmentally sustainable transport (EST) doesn't mean less transport and mobility than we have today, but it means primarily maintaining a balanced modal split that results in less environmental and health impacts than it would be under projected future trends. Rail, trams, busses and new forms of flexible inter-modal public transport mobility would have to take a large share and rail transport for passenger and freight as well as inland shipping would have to be nearly doubled by 2030 while road freight could still increase if it is based on alternative fuels reducing its impacts. Technological advancements for passenger cars and lorries, fuels and infrastructure will play an important part to achieve EST, but also ''smart'' mobility management (e.g. transport avoidance, increasing load factors and modal shift), innovative mobility services and freight logistics would be critical. The implementation of these policies and strategies will require coherent and comprehensive packages of instruments and measures, including: economic instruments, regulatory instruments, changes in infrastructure investment, mobility management, information and education programmes as well as better integration of land use, transport and environment policies. Realising EST will provide new opportunities for businesses to develop and invest in innovative solutions for passenger and freight transport. Overall, achieving EST would constitute a net benefit for

  13. Identification of four nuclear transport signal-binding proteins that interact with diverse transport signals.

    Science.gov (United States)

    Yamasaki, L; Kanda, P; Lanford, R E

    1989-07-01

    The transport of proteins into the nucleus requires not only the presence of a nuclear transport signal on the targeted protein but also the signal recognition proteins and the nuclear pore translocation apparatus. Complicating the search for the signal recognition proteins is the fact that the nuclear transport signals identified share little obvious homology. In this study, synthetic peptides homologous to the nuclear transport signals from the simian virus 40 large T antigen, Xenopus oocyte nucleoplasmin, adenovirus E1A, and Saccharomyces cerevisiae MAT alpha 2 proteins were coupled to a UV-photoactivable cross-linker and iodinated for use in an in vitro cross-linking reaction with cellular lysates. Four proteins, p140, p100, p70, and p55, which specifically interacted with the nuclear transport signal peptides were identified. Unique patterns of reactivity were observed with closely related pairs of nuclear transport signal peptides. Competition experiments with labeled and unlabeled peptides demonstrated that heterologous signals were able to bind the same protein and suggested that diverse signals use a common transport pathway. The subcellular distribution of the four nuclear transport signal-binding proteins suggested that nuclear transport involves both cytoplasmic and nuclear receptors. The four proteins were not bound by wheat germ agglutinin and were not associated tightly with the nuclear pore complex.

  14. 14 CFR 147.23 - Instructor requirements.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Instructor requirements. 147.23 Section 147.23 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) SCHOOLS AND OTHER CERTIFICATED AGENCIES AVIATION MAINTENANCE TECHNICIAN SCHOOLS Certification...

  15. 14 CFR 91.405 - Maintenance required.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 2 2010-01-01 2010-01-01 false Maintenance required. 91.405 Section 91.405 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIR TRAFFIC AND GENERAL OPERATING RULES GENERAL OPERATING AND FLIGHT RULES Maintenance, Preventive...

  16. 9 CFR 83.6 - Testing requirements.

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Testing requirements. 83.6 Section 83.6 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE INTERSTATE TRANSPORTATION OF ANIMALS (INCLUDING POULTRY) AND ANIMAL PRODUCTS VIRAL HEMORRHAGIC...

  17. 14 CFR 152.413 - Notice requirement.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Notice requirement. 152.413 Section 152.413 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIRPORTS... shall, on the grounds of race, creed, color, national origin, or sex, be excluded from participating...

  18. 14 CFR 147.3 - Certificate required.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Certificate required. 147.3 Section 147.3 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) SCHOOLS AND OTHER CERTIFICATED AGENCIES AVIATION MAINTENANCE TECHNICIAN SCHOOLS General § 147.3 Certificate...

  19. 10 CFR 490.803 - Waiver requirements.

    Science.gov (United States)

    2010-01-01

    ... 10 Energy 3 2010-01-01 2010-01-01 false Waiver requirements. 490.803 Section 490.803 Energy DEPARTMENT OF ENERGY ENERGY CONSERVATION ALTERNATIVE FUEL TRANSPORTATION PROGRAM Alternative Compliance § 490... as specified in § 490.804 of this subpart, equal to the amount of alternative fuel used if...

  20. 49 CFR 533.5 - Requirements.

    Science.gov (United States)

    2010-10-01

    ..., DEPARTMENT OF TRANSPORTATION LIGHT TRUCK FUEL ECONOMY STANDARDS § 533.5 Requirements. (a) Each manufacturer... a manufacturer's option, a manufacturer's light truck fleet may comply with the fuel economy level...'s light truck fleet shall comply with the fuel economy level calculated for that model...