Sample records for anoxybacillus flavithermus wk1

  1. Encapsulated in silica: genome, proteome and physiology of the thermophilic bacterium Anoxybacillus flavithermus WK1


    Saw, Jimmy H; Mountain, Bruce W; Feng, Lu; Omelchenko, Marina V; Hou, Shaobin; Saito, Jennifer A.; Stott, Matthew B.; Li, Dan; Zhao, Guang; Wu, Junli; Galperin, Michael Y.; Koonin, Eugene V.; Makarova, Kira S.; Wolf, Yuri I; Rigden, Daniel J.


    Background Gram-positive bacteria of the genus Anoxybacillus have been found in diverse thermophilic habitats, such as geothermal hot springs and manure, and in processed foods such as gelatin and milk powder. Anoxybacillus flavithermus is a facultatively anaerobic bacterium found in super-saturated silica solutions and in opaline silica sinter. The ability of A. flavithermus to grow in super-saturated silica solutions makes it an ideal subject to study the processes of sinter formation, whic...

  2. Encapsulated in silica: genome, proteome and physiology of the thermophilic bacterium Anoxybacillus flavithermus

    Energy Technology Data Exchange (ETDEWEB)

    Saw, Jimmy H [Los Alamos National Laboratory; Mountain, Bruce W [NEW ZEALAND; Feng, Lu [NANKAI UNIV; Omelchenko, Marina V [NCBI/NLM/NIH; Hou, Shaobin [UNIV OF HAWAII; Saito, Jennifer A [UNIV OF HAWAII; Stott, Matthew B [NEW ZEALAND; Li, Dan [NANKAI UNIV; Zhao, Guang [NANKAI UNIV; Wu, Junli [NANKAI UNIV; Galperin, Michael Y [NCBI/NLM/NIH; Koonin, Eugene V [NCBI/NLM/NIH; Makarova, Kira S [NCBI/NLM/NIH; Wolf, Yuri I [NCBI/NLM/NIH; Rigden, Daniel J [UNIV OF LIVERPOOL; Dunfield, Peter F [UNIV OF CALGARY; Wang, Lei [NANKAI UNIV; Alam, Maqsudul [UNIV OF HAWAII


    Gram-positive bacteria of the genus Anoxybacillus have been found in diverse thermophilic habitats, such as geothermal hot springs and manure, and in processed foods such as gelatin and milk powder. Anoxybacillus flavithermus is a facultatively anaerobic bacterium found in super-saturated silica solutions and in opaline silica sinter. The ability of A. flavithermus to grow in super-saturated silica solutions makes it an ideal subject to study the processes of sinter formation, which might be similar to the biomineralization processes that occurred at the dawn of life. We report here the complete genome sequence of A. flavithermus strain WK1, isolated from the waste water drain at the Wairakei geothermal power station in New Zealand. It consists of a single chromosome of 2,846,746 base pairs and is predicted to encode 2,863 proteins. In silico genome analysis identified several enzymes that could be involved in silica adaptation and biofilm formation, and their predicted functions were experimentally validated in vitro. Proteomic analysis confirmed the regulation of biofilm-related proteins and crucial enzymes for the synthesis of long-chain polyamines as constituents of silica nanospheres. Microbial fossils preserved in silica and silica sinters are excellent objects for studying ancient life, a new paleobiological frontier. An integrated analysis of the A. flavithermus genome and proteome provides the first glimpse of metabolic adaptation during silicification and sinter formation. Comparative genome analysis suggests an extensive gene loss in the Anoxybacillus/Geobacillus branch after its divergence from other bacilli.

  3. Genome Sequence of Anoxybacillus flavithermus Strain AK1, a Thermophile Isolated from a Hot Spring in Saudi Arabia

    KAUST Repository

    Khalil, Amjad


    Anoxybacillus flavithermus strain AK1 was isolated from Al-Ain Alhara, a thermal hot spring located 50 km southeast of the city of Gazan, Saudi Arabia (16°56ʹN, 43°15ʹE). The sequenced and annotated genome is 2,630,664 bp and encodes 2,799 genes.

  4. Draft Genome Sequences of Seven Thermophilic Spore-Forming Bacteria Isolated from Foods That Produce Highly Heat-Resistant Spores, Comprising Geobacillus spp., Caldibacillus debilis, and Anoxybacillus flavithermus (United States)

    Berendsen, Erwin M.; Wells-Bennik, Marjon H. J.; Krawczyk, Antonina O.; de Jong, Anne; van Heel, Auke; Holsappel, Siger; Eijlander, Robyn T.


    Here, we report the draft genomes of five strains of Geobacillus spp., one Caldibacillus debilis strain, and one draft genome of Anoxybacillus flavithermus, all thermophilic spore-forming Gram-positive bacteria. PMID:27151781

  5. Demonstration of the Principles of Restriction Endonuclease Cleavage Reactions Using Thermostable Bfl I from "Anoxybacillus Flavithermus" (United States)

    Sharma, Prince; D'Souza, David R.; Bhandari, Deepali; Parashar, Vijay; Capalash, Neena


    Restriction enzymes are basic tools in recombinant DNA technology. To shape the molecular biology experiments, the students must know how to work with these molecular scissors. Here, we describe an integrated set of experiments, introduced in the "Advances in Molecular Biology and Biotechnology" postgraduate course, which covers the important…

  6. AcEST: DK949774 [AcEST

    Lifescience Database Archive (English)

    Full Text Available TST38A01NGRL0006_O06 674 Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0006_O06. 5' end seq ... |B7GKL4_9BACI Uncharacterized protein conserved in bacteria ... OS=Anoxybacillus flavithermus WK1 GN=Aflv_2399 PE= ...

  7. Aerobic growth of Anoxybacillus pushchinoensis K1(T): emended descriptions of A. pushchinoensis and the genus Anoxybacillus (United States)

    Pikuta, Elena; Cleland, David; Tang, Jane


    In this work, corrections are made to the descriptions of the species Anoxybacillus pushchinoensis corrig. and the genus ANOXYBACILLUS: Experiments to determine the relationship of A. pushchinoensis K1(T) to oxygen showed that it was capable of aerobic growth, but preferred to grow anaerobically. During aerobic growth, the redox indicator resazurin was reduced as a result of hydrogen gas production. The facultatively anaerobic nature of K1(T) was ascertained by cultivation in aerobic liquid medium, where growth began at the bottom of the tube. The anaerobic nature of K1(T) was also indicated by a negative catalase reaction. This work is submitted to correct the description of the species A. pushchinoensis from obligate anaerobe to aerotolerant anaerobe and to emend the description of the genus Anoxybacillus from obligate anaerobes or facultative anaerobes to aerotolerant anaerobes or facultative anaerobes.

  8. Chemolithoautotrophic arsenite oxidation by a thermophilic Anoxybacillus flavithermus strain TCC9-4 from a hot spring in Tengchong of Yunnan, China


    Jiang, Dawei; Li, Ping; Jiang, Zhou; Dai, Xinyue; Zhang, Rui; Wang, Yanhong; Guo, Qinghai; Wang, Yanxin


    A new facultative chemolithoautotrophic arsenite (AsIII)-oxidizing bacterium TCC9-4 was isolated from a hot spring microbial mat in Tengchong of Yunnan, China. This strain could grow with AsIII as an energy source, CO2–HCO3- as a carbon source and oxygen as the electron acceptor in a minimal salts medium. Under chemolithoautotrophic conditions, more than 90% of 100 mg/L AsIII could be oxidized by the strain TCC9-4 in 36 h. Temperature was an important environmental factor that strongly influe...

  9. LC-ESI-MS/MS Identification of Polar Lipids of Two Thermophilic Anoxybacillus Bacteria Containing a Unique Lipid Pattern

    Czech Academy of Sciences Publication Activity Database

    Řezanka, Tomáš; Kambourova, M.; Derekova, A.; Kolouchová, I.; Sigler, Karel


    Roč. 47, č. 7 (2012), s. 729-739. ISSN 0024-4201 R&D Projects: GA ČR(CZ) GAP503/11/0215 Institutional support: RVO:61388971 Keywords : Anoxybacillus * Plasmalogens * O-Aminoacylphosphatidylglycerols Subject RIV: CE - Biochemistry Impact factor: 2.557, year: 2012

  10. Crystal structure of Anoxybacillus α-amylase provides insights into maltose binding of a new glycosyl hydrolase subclass. (United States)

    Chai, Kian Piaw; Othman, Noor Farhan Binti; Teh, Aik-Hong; Ho, Kok Lian; Chan, Kok-Gan; Shamsir, Mohd Shahir; Goh, Kian Mau; Ng, Chyan Leong


    A new subfamily of glycosyl hydrolase family GH13 was recently proposed for α-amylases from Anoxybacillus species (ASKA and ADTA), Geobacillus thermoleovorans (GTA, Pizzo, and GtamyII), Bacillus aquimaris (BaqA), and 95 other putative protein homologues. To understand this new GH13 subfamily, we report crystal structures of truncated ASKA (TASKA). ASKA is a thermostable enzyme capable of producing high levels of maltose. Unlike GTA, biochemical analysis showed that Ca(2+) ion supplementation enhances the catalytic activities of ASKA and TASKA. The crystal structures reveal the presence of four Ca(2+) ion binding sites, with three of these binding sites are highly conserved among Anoxybacillus α-amylases. This work provides structural insights into this new GH13 subfamily both in the apo form and in complex with maltose. Furthermore, structural comparison of TASKA and GTA provides an overview of the conformational changes accompanying maltose binding at each subsite. PMID:26975884

  11. A High Molecular-Mass Anoxybacillus sp. SK3-4 Amylopullulanase: Characterization and Its Relationship in Carbohydrate Utilization


    Kian Mau Goh; Siew Mee Hii; Ummirul Mukminin Kahar; Madihah Md. Salleh; Kok-Gan Chan


    An amylopullulanase of the thermophilic Anoxybacillus sp. SK3-4 (ApuASK) was purified to homogeneity and characterized. Though amylopullulanases larger than 200 kDa are rare, the molecular mass of purified ApuASK appears to be approximately 225 kDa, on both SDS-PAGE analyses and native-PAGE analyses. ApuASK was stable between pH 6.0 and pH 8.0 and exhibited optimal activity at pH 7.5. The optimal temperature for ApuASK enzyme activity was 60 °C, and it retained 54% of its total activity for 2...

  12. Purification and characterization of an extracellular cellulase from Anoxybacillus gonensis O9 isolated from geothermal area in Turkey. (United States)

    Genc, Berna; Nadaroglu, Hayrunnisa; Adiguzel, Ahmet; Baltaci, Ozkan


    In the present study, cellulase was purified and characterized from Anoxybacillus gonensis (Gen bank Number: KM596794) which was isolated and characterized from Agri Diyadin Hot spring. It was found to synthesize cellulase which had a wide range of industrial applications. Twenty four-hour-cultured bacteria induced cellulase production and specific activities during the purification steps were 1.47, 81.06 and 109.4 EU mg(-1) protein at crude extract, ammonium sulphate precipitated and DEAE-Sephadex purification steps. The highest enzyme activity was observed at 50°C and the optimum range of pH was 3-10. Molecular weight of enzyme was determined approximately 40kDa. The kinetic parameters of cellulase against carboxymethylcellulose (CMC) were 153.4 pmol min(-1) mg for Vmax and 0.46mM for Km. Among effectors of the enzyme, Zn2+, Ca2+, Co2+ and EDTA decreased enzyme activity. PMID:26688967

  13. A High Molecular-Mass Anoxybacillus sp. SK3-4 Amylopullulanase: Characterization and Its Relationship in Carbohydrate Utilization

    Directory of Open Access Journals (Sweden)

    Kian Mau Goh


    Full Text Available An amylopullulanase of the thermophilic Anoxybacillus sp. SK3-4 (ApuASK was purified to homogeneity and characterized. Though amylopullulanases larger than 200 kDa are rare, the molecular mass of purified ApuASK appears to be approximately 225 kDa, on both SDS-PAGE analyses and native-PAGE analyses. ApuASK was stable between pH 6.0 and pH 8.0 and exhibited optimal activity at pH 7.5. The optimal temperature for ApuASK enzyme activity was 60 °C, and it retained 54% of its total activity for 240 min at 65 °C. ApuASK reacts with pullulan, starch, glycogen, and dextrin, yielding glucose, maltose, and maltotriose. Interestingly, most of the previously described amylopullulanases are unable to produce glucose and maltose from these substrates. Thus, ApuASK is a novel, high molecular-mass amylopullulanase able to produce glucose, maltose, and maltotriose from pullulan and starch. Based on whole genome sequencing data, ApuASK appeared to be the largest protein present in Anoxybacillus sp. SK3-4. The α-amylase catalytic domain present in all of the amylase superfamily members is present in ApuASK, located between the cyclodextrin (CD-pullulan-degrading N-terminus and the α-amylase catalytic C-terminus (amyC domains. In addition, the existence of a S-layer homology (SLH domain indicates that ApuASK might function as a cell-anchoring enzyme and be important for carbohydrate utilization in a streaming hot spring.

  14. Characterization of a type I pullulanase from Anoxybacillus sp. SK3-4 reveals an unusual substrate hydrolysis. (United States)

    Kahar, Ummirul Mukminin; Ng, Chyan Leong; Chan, Kok-Gan; Goh, Kian Mau


    Type I pullulanases are enzymes that specifically hydrolyse α-1,6 linkages in polysaccharides. This study reports the analyses of a novel type I pullulanase (PulASK) from Anoxybacillus sp. SK3-4. Purified PulASK (molecular mass of 80 kDa) was stable at pH 5.0-6.0 and was most active at pH 6.0. The optimum temperature for PulASK was 60 °C, and the enzyme was reasonably stable at this temperature. Pullulan was the preferred substrate for PulASK, with 89.90 % adsorbance efficiency (various other starches, 56.26-72.93 % efficiency). Similar to other type I pullulanases, maltotriose was formed on digestion of pullulan by PulASK. PulASK also reacted with β-limit dextrin, a sugar rich in short branches, and formed maltotriose, maltotetraose and maltopentaose. Nevertheless, PulASK was found to preferably debranch long branches at α-1,6 glycosidic bonds of starch, producing amylose, linear or branched oligosaccharides, but was nonreactive against short branches; thus, no reducing sugars were detected. This is surprising as all currently known type I pullulanases produce reducing sugars (predominantly maltotriose) on digesting starch. The closest homologue of PulASK (95 % identity) is a type I pullulanase from Anoxybacillus sp. LM14-2 (Pul-LM14-2), which is capable of forming reducing sugars from starch. With rational design, amino acids 362-370 of PulASK were replaced with the corresponding sequence of Pul-LM14-2. The mutant enzyme formed reducing sugars on digesting starch. Thus, we identified a novel motif involved in substrate specificity in type I pullulanases. Our characterization may pave the way for the industrial application of this unique enzyme. PMID:27000839

  15. Pročišćavanje i karakterizacija α-amilaze izolirane iz soja Anoxybacillus sp. AH1, stabilne pri visokim temperaturama i u prisutnosti detergenata


    Acer, Ömer; Matpan Bekler, Fatma; Pirinççioğlu, Hemşe; Gül Güven, Reyhan; Güven, Kemal


    U radu je pročišćena i okarakterizirana α-amilaza, izolirana iz novootkrivenog soja bakterije Anoxybacillus sp. AH1, stabilna pri visokim temperaturama u prisutnosti detergenata. Najveća količina enzima (1874,8 U/mL) proizvedena je nakon 24 sata inkubacije. Nakon pročišćavanja amilaze gel-filtracijskom kromatografijom u koloni punjenoj gelom Sephadex G-75, specifična se aktivnost enzima povećala 18 puta, a prinos je bio 9 %. Metodom SDS-PAGE određena je molekularna masa pročišćenog enzima od ...

  16. Draft Genome Sequences of Four Thermophilic Spore Formers Isolated from a Dairy-Processing Environment (United States)

    Caspers, Martien P. M.; Boekhorst, Jos; de Jong, Anne; Kort, Remco; Nierop Groot, Masja


    Spores of thermophilic spore-forming bacteria are a common cause of contamination in dairy products. Here, we report draft genome sequences of four thermophilic strains from a milk-processing plant or standard milk, namely, a Geobacillus thermoglucosidans isolate (TNO-09.023), Geobacillus stearothermophilus TNO-09.027, and two Anoxybacillus flavithermus isolates (TNO-09.014 and TNO-09.016). PMID:27516503

  17. Abiotic and microbiotic factors controlling biofilm formation by thermophilic sporeformers. (United States)

    Zhao, Yu; Caspers, Martien P M; Metselaar, Karin I; de Boer, Paulo; Roeselers, Guus; Moezelaar, Roy; Nierop Groot, Masja; Montijn, Roy C; Abee, Tjakko; Kort, Remco


    One of the major concerns in the production of dairy concentrates is the risk of contamination by heat-resistant spores from thermophilic bacteria. In order to acquire more insight in the composition of microbial communities occurring in the dairy concentrate industry, a bar-coded 16S amplicon sequencing analysis was carried out on milk, final products, and fouling samples taken from dairy concentrate production lines. The analysis of these samples revealed the presence of DNA from a broad range of bacterial taxa, including a majority of mesophiles and a minority of (thermophilic) spore-forming bacteria. Enrichments of fouling samples at 55°C showed the accumulation of predominantly Brevibacillus and Bacillus, whereas enrichments at 65°C led to the accumulation of Anoxybacillus and Geobacillus species. Bacterial population analysis of biofilms grown using fouling samples as an inoculum indicated that both Anoxybacillus and Geobacillus preferentially form biofilms on surfaces at air-liquid interfaces rather than on submerged surfaces. Three of the most potent biofilm-forming strains isolated from the dairy factory industrial samples, including Geobacillus thermoglucosidans, Geobacillus stearothermophilus, and Anoxybacillus flavithermus, have been characterized in detail with respect to their growth conditions and spore resistance. Strikingly, Geobacillus thermoglucosidans, which forms the most thermostable spores of these three species, is not able to grow in dairy intermediates as a pure culture but appears to be dependent for growth on other spoilage organisms present, probably as a result of their proteolytic activity. These results underscore the importance of abiotic and microbiotic factors in niche colonization in dairy factories, where the presence of thermophilic sporeformers can affect the quality of end products. PMID:23851093

  18. Innovative enzymes for bioethanol production from lignocellulosic materials


    Marcolongo, Loredana


    The general aim of this work was to add new knowledge on novel hemicellulolytic enzymes involved in the hydrolysis of lignocellulosic materials, considered as a key process for the bioethanol production. Therefore, it is not only focused on (hemi)cellulolytic enzymes from mesophilic fungi and bacteria but also on newly isolated and characterized xylanase and β-xylosidase from the thermophilic bacteria Geobacillus thermodenitrificans A333 and Anoxybacillus sp. 3M, respectively. The cove...

  19. Isolation and characterization of a radiation resistant thermophilic bacterium from radon hot spring

    International Nuclear Information System (INIS)

    A radiation resistant and thermophilic bacterium strain R4-33 was isolated from radon hot spring water samples, pretreated with 60Co γ-rays and UV irradiation. Tests on morphological, physiological and biochemical characters, fatty acid compositions, (G + C) mol% contents, and 16S rDNA sequencing were conducted. The results showed that strain R4-33 was of rod-shape, Gram-negative, atrichous, and endospore-forming. The optimum growth temperature and pH were 60 ℃ and 7.5, respectively. The strain utilized glucose, maltose and trehalose as carbon sources, and hydrolyzed casein and starch. Its catalase positive. The strain was sensitive to penicillin, neomycin, erythromycin, vancomycin, streptomycin, gentamycin, amikacin and ampicillin. The major cellular fatty acids were C14:1 (48.8%) and C15:1 (15.2%). The (G + C) mol% content of DNA was 58.2%. Phylogenetic tree based on 16S rDNA sequence showed R4-33 shared highly similarity to those of species in genus Anoxybacillus, especially to that of Anoxybacillus gonensis (99.5%). Based on the above, the strain R4-33 was proposed to the evolution branch of Anoxybacillus and designated as Anoxybacillu sp. R4-33. The UV and γ-radiation tests showed that the strain R4-33 had an ability of resistance to UV of 396 J/m2 and 60Co γ-rays irradiation of 14.0 kGy, indicating that the strain was a radiation resistant and thermophilic bacterium. (authors)

  20. Dicty_cDB: CFI367 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available E Sequences producing significant alignments: (bits) Value CP000922_1494( CP000922 |pid:none) Anoxybacillus flav...E Sequences producing significant alignments: (bits) Value CFI367 (CFI367Q) /CSM/CF/CFI3-C/CFI367Q.Seq.d/ 25.../VFL750Q.Seq.d/ 979 0.0 own update 2009. 4. 4 Homology vs DNA Score E Sequences producing significant alignments: (bits...0646 |AY330646.1 Dictyostelium clavatum 18S ribosomal RNA gene, partial sequence; internal transcribed space...qshmvih*vqlvvfld*ngkxirkik*kirtsnnvyglvkqrnn Translated Amino Acid sequence (All Frames) Frame A: a*tvnts

  1. Analysis of early lactation reproductive characteristics in Holstein cows.


    Walters, Anneke H.


    Ultrasound-guided transvaginal follicular aspiration was used to obtain oocytes from cows to study follicular development and oocyte morphology. Follicular aspiration was conducted once during wk 1 to 12 postpartum on 120 lactating cows with 6 groups, separated by biweekly intervals. Approximately one half of the aspirated cows at each session were from the early groups (wk 1-2, 3-4, or 5-6) and the other half from the later groups (wk 7-8, 9-10, or 11-12). On the day of aspiration the numbe...

  2. Impact of sow and litter characteristics on colostrum yield for onset of lactation, and milk yield of sows

    DEFF Research Database (Denmark)

    Vadmand, C N; Larsen, Uffe Krogh; Hansen, C F;


    experiments were used. The variables describing sow productivity were all defined as dependent variables and Pearson coefficient of correlation was used to examine relations among dependent variables. The results showed that CY was positively correlated with transition MY and MY in wk 1 and 2 of lactation (P...

  3. A Novel WRKY transcription factor is required for induction of PR-1a gene expression by salicylic acid and bacterial elicitors

    NARCIS (Netherlands)

    van Verk, Marcel C; Pappaioannou, Dimitri; Neeleman, Lyda; Bol, John F; Linthorst, Huub J M


    PR-1a is a salicylic acid-inducible defense gene of tobacco (Nicotiana tabacum). One-hybrid screens identified a novel tobacco WRKY transcription factor (NtWRKY12) with specific binding sites in the PR-1a promoter at positions -564 (box WK(1)) and -859 (box WK(2)). NtWRKY12 belongs to the class of t

  4. Early- and traditionally weaned nursery pigs benefit from phase-feeding pharmacological concentrations of zinc oxide: effect on metallothionein and mineral concentrations. (United States)

    Carlson, M S; Hill, G M; Link, J E


    Benefits of feeding pharmacological concentrations of zinc (Zn) provided by Zn oxide (ZnO) to 21-d conventionally weaned pigs in the nursery have been documented; however, several management questions remain. We conducted two experiments to evaluate the effect on growth from feeding 3,000 ppm Zn as ZnO during different weeks of the nursery period. In Exp. 1 (n = 138, 11.5 d of age, 3.8 kg BW) and Exp. 2 (n = 246, 24.5 d of age, 7.2 kg BW), pigs were fed either basal diets containing 100 ppm supplemental Zn (adequate) or the same diet with an additional 3,000 ppm Zn (high) supplied as ZnO. Pigs were fed four or two dietary phases in Exp. 1 and 2, respectively, that changed in dietary ingredients and nutrient content (lysine and crude protein) to meet the changing physiological needs of the pigs for the 28-d nursery period. Dietary Zn treatments were 1) adequate Zn fed wk 1 to 4, 2) high Zn fed wk 1, 3) high Zn fed wk 2, 4) high Zn fed wk 1 and 2, 5) high Zn fed wk 2 and 3, and 6) high Zn fed wk 1 to 4. In Exp. 1 and 2, pigs fed high Zn for wk 1 and 2 or the entire 28-d nursery period had the greatest (P pigs fed high Zn had greater concentrations of hepatic metallothionein and Zn in plasma, liver, and kidney than those pigs fed adequate Zn (P weaned pigs need to be fed pharmacological concentrations of Zn provided as ZnO for a minimum of 2 wk immediately after weaning to enhance growth. PMID:10340587

  5. Effects of activated sludge flocs and pellets seeds on aerobic granule properties

    Institute of Scientific and Technical Information of China (English)

    Huacheng Xu; Pinjing He; Guanzhao Wang; Liming Shao


    Aerobic granules seeded with activated sludge fiocs and pellets (obtained from activated sludge flocs) were cultivated in two sequencing batch reactors and their characteristics were compared.Compared with granules seeded with activated sludge flocs, those seeded with pellets had shorter start-up time, larger diameter, better chemical oxygen demand removal efficiency, and higher hydrophobicity, suspended solid concentration, and Mg2+ content.The different inocula led the granule surface with different microbial morphologies, but did not result in different distribution patterns of extracellular polymeric substances and cells.The anaerobic bacterium Anoxybacillus sp.was detected in the granules seeded with pellets.These results highlighted the advantage of pellet over activated sludge floc as the seed for aerobic granulation and wastewater treatment.

  6. Correlations between periparturient serum concentrations of non-esterified fatty acids, beta-hydroxybutyric acid, bilirubin, and urea and the occurrence of clinical and subclinical postpartum bovine endometritis

    Directory of Open Access Journals (Sweden)

    Tenhagen Bernd-Alois


    Full Text Available Abstract Background Postpartum endometritis in cattle is a multifactorial disease with high economic impact. Both, clinical endometritis (CE and subclinical endometritis (SCE result in decreased reproductive performance. Results from in vitro studies led to the implication that non-esterified fatty acids (NEFA, beta-hydroxybutyric acid (BHBA, bilirubin, and urea could be used as predictors for endometritis in veterinary practice. In this field study, we set out to establish optimal predictor cut points of these metabolic parameters for the detection of CE and SCE. Serum samples were collected one week prior to parturition (wk -1, in the first week postpartum (wk +1 and between 28 and 35 days postpartum (wk +5 from 209 Holstein-Friesian cows. At wk +5, all cows were examined for signs of CE and SCE. Results Higher concentrations of urea at wk +1 were associated with increased odds of CE (OR = 1.7, P = 0.04 in primiparous (PP cows. A predictor cut point of 3.9 mmol/L (sensitivity: 61%, specificity: 70% was determined. In multiparous (MP cows, the logistic regression model revealed that higher concentrations of NEFA at wk -1 were associated with increased odds of CE and SCE (healthy vs. CE: OR = 9.1, P = 0.05; healthy vs. SCE: OR = 12.1, P = 0.04. A predictor cut point of 0.3 mmol/L (sensitivity: 38%, specificity: 87% and sensitivity: 35%, specificity: 89%, respectively was determined. Increasing concentrations of urea at wk +5 were associated with decreased odds of CE (healthy vs. CE: OR = 0.6, P = 0.01; SCE vs. CE: OR = 0.5, P = 0.03. A predictor cut point of 3.8 mmol/L (sensitivity: 52%, specificity: 81% was determined. For BHBA and bilirubin relationships with CE or SCE were not detected. Conclusions The corresponding combinations of sensitivity and specificity of the determined predictor cut points were not satisfactory for practical use. Thus, the analysed parameters, i.e. NEFA, BHBA, bilirubin, and urea, at the chosen time points, i.e. at wk

  7. Risk factors that affect reproductive target achievement in fertile dairy cows. (United States)

    Aungier, S P M; Roche, J F; Diskin, M G; Crowe, M A


    The aims of the present study were to investigate (1) the risk factors that influence the achievement of reproductive targets postpartum (pp) and (2) the key factors that influence pregnancy rate following first artificial insemination (AI) in dairy cows. Ninety-eight Holstein-Friesian pp cows were blood sampled from wk 1 to 4 pp for hematology and biochemistry. Reproductive tract health was assessed weekly by ultrasonography and vaginal mucus scoring. Body condition score (BCS), lameness score, and milk yield were assessed every 2 wk. Milk samples for progesterone assay were collected twice weekly and on d 4, 5, and 7 after AI. Risk factors associated with achieving reproductive targets depended on (1) increased metabolic activity of the liver (increased glutamate dehydrogenase at calving and increased γ-glutamyl transpeptidase in wk 4), (2) a competent immune system (increased neutrophils in wk 1; decreased α1-acid glycoprotein in wk 1, 2, and 3), (3) an endocrine system that was capable of responding by producing sufficient triiodothyronine in wk 2 and increased insulin-like growth factor I in wk 3 and 4, (4) a lower negative energy balance status (decreased nonesterified fatty acid concentration in wk 1; decreased β-hydroxybutyrate concentration in wk 2; BCS loss between calving and d 28 pp factors that increased the odds of a successful first AI were previous ovulation(s) (odds ratio=3.17 per ovulation), BCS >2.5 at AI (odds ratio=3.01), and clear vaginal mucus (score=0) compared with purulent mucus (score >0) 4 d after first AI (odds ratio=2.99). In conclusion, this study identified key risk factors in the early pp period that give a higher probability of cows achieving their reproductive targets and of having a first-AI pregnancy. PMID:24679929

  8. Intravaginal Lactic Acid Bacteria Modulated Local and Systemic Immune Responses and Lowered the Incidence of Uterine Infections in Periparturient Dairy Cows


    Deng, Qilan; Odhiambo, John F.; Farooq, Umar; Lam, Tran; Dunn, Suzanna M.; Ametaj, Burim N.


    The objective of this investigation was to evaluate whether intravaginal infusion of a lactic acid bacteria (LAB) cocktail around parturition could influence the immune response, incidence rate of uterine infections, and the overall health status of periparturient dairy cows. One hundred pregnant Holstein dairy cows were assigned to 1 of the 3 experimental groups as follows: 1) one dose of LAB on wk -2 and -1, and one dose of carrier (sterile skim milk) on wk +1 relative to the expected day o...

  9. Muscular and Systemic Correlates of Resistance Training-Induced Muscle Hypertrophy


    Mitchell, Cameron J.; Churchward-Venne, Tyler A.; Bellamy, Leeann; Parise, Gianni; Baker, Steven K.; Stuart M. Phillips


    Purpose To determine relationships between post-exercise changes in systemic [testosterone, growth hormone (GH), insulin like grow factor 1 (IGF-1) and interleukin 6 (IL-6)], or intramuscular [skeletal muscle androgen receptor (AR) protein content and p70S6K phosphorylation status] factors in a moderately-sized cohort of young men exhibiting divergent resistance training-mediated muscle hypertrophy. Methods Twenty three adult males completed 4 sessions•wk-1 of resistance training for 16 wk. M...

  10. Association of rumination time with subclinical ketosis in transition dairy cows. (United States)

    Kaufman, E I; LeBlanc, S J; McBride, B W; Duffield, T F; DeVries, T J


    The objective of this study was to characterize the relationship between rumination and subclinical ketosis (SCK) in transition dairy cows. A study was conducted on 4 commercial dairy farms in eastern Ontario, Canada. A total of 339 Holstein dairy cows (107 primiparous and 232 multiparous) were monitored for rumination activity and SCK from 14 d before calving until 28 d after calving. Rumination was recorded daily using an automated monitoring system. A blood sample was taken from the coccygeal vein of each cow for measurement of β-hydroxybutyrate (BHB) once weekly throughout the 6-wk observation period. Cows with BHB ≥1.2mmol/L in any of the 4 postpartum samples were considered to have SCK. Cases of retained placenta, metritis, milk fever, or mastitis during the study period were also recorded. Cows were categorized into 1 of 4 groups: healthy cows (HLT) that had no SCK or any other recorded health problem (n=139); cows treated for at least one health issue other than SCK (HLT+; n=50); cows with SCK (hyperketonemia; HYK) with no other health problems during transition (n=97); or cows (HYK+) that had SCK and one or more other health problems (n=53). Daily rumination time was summarized by week and comparisons were made between HLT and HYK and HYK+. From 2 wk before calving (wk -2) to 4 wk after calving (wk +4), there was no difference in rumination time (409±9.8min/d) among HLT, HYK, and HYK+ cows in their first lactation. Multiparous cows in HLT spent an average of 459±11.3min/d ruminating from wk -2 to wk +4. Multiparous HYK cows ruminated 25±12.8min/d less than HLT cows, whereas HYK+ cows ruminated 44±15.6min/d less than HLT cows. The largest differences in rumination time between HLT and HYK+ cows were seen during wk -1, +1, and +2, when HYK+ cows ruminated 48±17.2, 73±16.0, and 65±19.4min/d less than HLT cows, respectively. In multiparous cows, increased odds of HYK were associated with greater milk yield in the previous lactation, greater loss of


    Institute of Scientific and Technical Information of China (English)

    梁新乐; 杨龙; 张虹; 张蕾


    氡温泉水样经8kGy60Co γ射线预处理后,从中分离获得1株耐60Co γ射线和UV辐照的嗜热菌株R4-33.经形态观察、生理生化试验、脂肪酸分析、(G+C)mol%含量和16S rDNA序列测定,结果表明,菌株R4-33为杆状,革兰氏阴性菌,无鞭毛,形成末端芽孢;最适生长温度60℃,最适生长pH 7.5;能以葡萄糖、麦芽糖和海藻糖等作为唯一碳源生长,水解酪素和淀粉;过氧化氢酶阳性;对青霉素、新霉素、红霉素、万古霉素、链霉素、庆大霉素、丁胺卡拉和氨苄青霉素敏感;细胞主要脂肪酸为C14:1(48.4%)和C15:1(15.2%);基因组DNA中(G+C)mo1%含量58.2%.经比对菌株R4-33的16SrDNA序列与Anoxybacillus属菌株有较高的同源性,其中与Anoxybacillus gonensis菌株的相似性高达99.5%.经R4-33菌株生理生化试验及16S rDNA序列分析,该株菌归属于Anoxybacillus属,暂命名为Anoxybacillus sp.R4-33.耐辐射细胞存活试验结果显示菌株R4-33对UV的耐辐射能力达396J/m2,对60Coγ射线耐辐照能力为14kGy,表明其对UV和γ射线辐照具有较高耐受性.%A radiation resistant and thermophilic bacterium strain R4 - 33 was isolated from radon hot spring water samples, pretreated with 60Co -γ - rays and UV irradiation. Tests on morphological, physiological and biochemical characters, fatty acid compositions, (G + C) mol% contents, and 16S rDNA sequencing were conducted. The results showed that strain R4 -33 was of rod-shape, Gram-negative, atrichous, and endospore-forming. The optimum growth temperature and pH were 60°C and 7.5, respectively. The strain utilized glucose, maltose and trehalose as carbon sources,and hydrolyzed casein and starch. Its catalase positive. The strain was sensitive to penicillin, neomycin, erythromycin, vancomycin, streptomycin, gentamycin, amikacin and ampicillin. The major cellular fatty acids were C14:l (48. 4% ) and C15:1 (15. 2% ). The ( G + C) mol% content of DNA was 58. 2% . Phylogenetic tree based on

  12. Ageing and Chronic Administration of Serotonin-Selective Reuptake Inhibitor Citalopram Upregulate Sirt4 Gene Expression in the Preoptic Area of Male Mice

    Directory of Open Access Journals (Sweden)

    Wong eDutt Way


    Full Text Available Sexual dysfunction and cognitive deficits are markers of the ageing process. Mammalian sirtuins (SIRT, encoded by sirt 1-7 genes, are known as ageing molecules which are sensitive to serotonin (5-hydroxytryptamine, 5-HT. Whether the 5-HT system regulates SIRT in the preoptic area (POA, which could affect reproduction and cognition has not been examined. Therefore, this study was designed to examine the effects of citalopram (CIT, 10mg/kg for 4 weeks, wk, a potent selective-serotonin reuptake inhibitor and ageing on SIRT expression in the POA of male mice using real-time PCR and immunocytochemistry. Age-related increases of sirt1, sirt4, sirt5, and sirt7 mRNA levels were observed in the POA of 52 wk old mice. Furthermore, 4 wk of chronic CIT treatment started at 8 wk of age also increased sirt2 and sirt4 mRNA expression in the POA. Moreover, the number of SIRT4 immuno-reactive neurons increased with ageing in the medial septum area (12 wk = 1.00±0.15 vs 36 wk = 1.68±0.14 vs 52 wk = 1.54±0.11, p<0.05. In contrast, the number of sirt4-immunopositive cells did not show a statistically significant change with CIT treatment, suggesting that the increase in sirt4 mRNA levels may occur in cells in which sirt4 is already being expressed. Taken together, these studies suggest that CIT treatment and the process of ageing utilize the serotonergic system to up-regulate SIRT4 in the POA as a common pathway to deregulate social cognitive and reproductive functions.

  13. Proliferation of L02 human hepatocytes in tolerized geneticall yimmunocompetent rats

    Institute of Scientific and Technical Information of China (English)

    Hu Lin; Qing Mao; Yu-Ming Wang; Li Jiang


    AIM: To investigate whether human hepatocytes could proliferate after transplantation to normal immunocompetent rats treated with 2-acetaminofluorene or Retrorsine and partial hepatectomy.METHODS: L02 hepatocyte-tolerant Sprague-Dawley rats were injected with Retrorsine, 2-acetaminofluorene or normal saline. L02 hepatocytes were then transplanted via the spleen. Human albumin and its mRNA, specific proliferating cell nuclear antigen (PCNA), L02 hepatocyte dynamic distribution, number density and area density of PCNA-positive cells in the liver were determined.RESULTS: All the examined indicators were not significantly different between the rats treated with 2-acetaminofluorene and normal saline, which was not the case with rats treated with Retrorsine. A dynamic distribution of L02 hepatocytes in the rat liver was detected from wk 1 to mo 6 after transplantation in the Retrorsine group and from wk 1 to 10 in the 2-acetaminofluorene group. Human albumin and its mRNA were detected from wk 2 to mo 6 in the Retrorsine group and from wk 1 to 8 in the 2-acetaminofiuorene group.Specific human PCNA was detected in the rat liver from wk 2 to mo 6 in the Retrorsine group and from wk 2 to 6 in the 2-acetaminofluorene group. Human albumin and its mRNA contents as well as the number of PCNA positive cells reached a peak at wk 4.CONCLUSION: L02 human hepatocytes could not proliferate significiantly after transplantation to the normal,immunocompetent rats treated with 2-acetaminofluorene.L02 human hepatocytes can survive for 10 wk after transplantation and express human albumin for 8 wk. L02human hepatocytes can proliferate and express human albumin for 6 mo after transplantation to the rats treated with Retrorsine. The chimeric L02 human hepatocytes,which then underwent transplantation into tolerant rats,were normal in morphogenesis, biochemistry and function.

  14. A low-glycemic index diet and exercise intervention reduces TNF(alpha) in isolated mononuclear cells of older, obese adults

    DEFF Research Database (Denmark)

    Kelly, Karen R; Haus, Jacob M; Solomon, Thomas;


    ; BMI: 34.2 ± 0.7 kg · m(-2)) to a 12-wk, low (LGI = 40) or high- (HGI = 80) glycemic index diet plus aerobic exercise (5 d · wk(-1), 60 min · d(-1), 80-85% heart rate(max)) intervention. All food and fluids were provided during the study. Inflammation was assessed from cytokine (TNFα and IL-6...... prevention of inflammation and hyperglycemia. A low-glycemic index diet has antiinflammatory and antidiabetogenic effects when combined with exercise in older, obese prediabetics....



    Olga Gonchar


    The aim of the present study was to examine the influence of intermittent hypoxia at rest and in combination with long-term high-intensity swimming exercise on lipid peroxidation and antioxidant defense system adaptation in skeletal muscles differing in fiber type composition. High-intensity chronic exercise was performed as swimming training with load that corresponded to ~ 75 % VO2max (30 min·day-1, 5 days·wk-1, for 4 wk). Intermittent hypoxic training (IHT) consisted of repeated episodes o...

  16. Growth Normal Faulting at the Western Edge of the Metropolitan Taipei Basin since the Last Glacial Maximum, Northern Taiwan

    Directory of Open Access Journals (Sweden)

    Chih-Tung Chen


    Full Text Available Growth strata analysis is an useful tool in understanding kinematics and the evolution of active faults as well as the close relationship between sedimentation and tectonics. Here we present the Shanchiao Fault as a case study which is an active normal fault responsible for the formation of the 700-m-thick late Quaternary deposits in Taipei Basin at the northern tip of the Taiwan mountain belt. We compiled a sedimentary record, particularly the depositional facies and their dated ages, at three boreholes (SCF-1, SCF-2 and WK-1, from west to east along the Wuku Profile that traverses the Shanchiao Fault at its central segment. By incorporating the global sea level change curve, we find that thickness changes of sediments and changes of depositional environments in the Wuku area are in a good agreement with a rapid sea level rise since the Last Glacial Maximum (LGM of about 23 ka. Combining depositional facies changes and their ages with their thickness, we are able to introduce a simple back-stripping method to reconstruct the evolution of growing strata across the Shanchiao Fault since the LGM. We then estimate the vertical tectonic slip rate since 23 ka, which exhibits 2.2 mm yr-1 between SCF-2 and WK-1 and 1.1 mm yr-1 between SCF-1 and SCF-2. We also obtain the Holocene tectonic subsidence rate of 2.3 mm yr-1 at WK-1 and 0.9 mm yr-1 at SCF-2 since 8.4 ka. We thus conclude that the fault zone consists of a high-angle main fault to the east between SCF-2 and WK-1 and a western lower-angle branch fault between SCF-1 and SCF-2, resembling a tulip structure developed under sinistral transtensional tectonism. We find that a short period of 600-yr time span in 9 - 8.4 ka shows important tectonic subsidence of 7.4 and 3.3 m for the main and branch fault, respectively, consistent with possible earthquake events proposed by previous studies during that time. A correlation between geomorphology and subsurface geology in the Shanchiao Fault zone shows

  17. Effects of high intensity interval training and high volume endurance training on maximal aerobic capacity, speed and power in club level gaelic football players


    Cregg, Cathal J


    Purpose: To compare the effects of high intensity interval training (HIIT) and high volume endurance training (HVET) on indices of endurance, speed and power in male Gaelic football players. Methods: Club level Gaelic football players (n=25) ranging from 18 to 35 years of age were randomly assigned to a HIIT (mean ± SD; 27.2 ± 3.6 yr) or a HVET ( mean ± SD 24.7 ± 4.0 yr) group. Participants trained 3 d.wk-1 for 6 weeks. Maximal aerobic capacity, vertical jump (VJ), countermovement jump (C...

  18. Molecular analysis of microbial community in arsenic-rich groundwater of Kolsor, West Bengal. (United States)

    Sarkar, Angana; Paul, Dhiraj; Kazy, Sufia K; Sar, Pinaki


    Bacterial community composition within the highly arsenic (As) contaminated groundwater from Kolsur, West Bengal was analyzed over a period of 3 years using 16S rRNA gene clone library and Denaturing Gradient Gel Electrophoresis (DGGE). Molecular phylogenetic study revealed abundance of α-Proteobacteria (56%) and Firmicutes (29%) along with members of β-Proteobacteria, Verrucomicrobia and Sphingobacteria as relatively minor groups. Along with consistent physicochemical environment, a stable microbial community structure comprising of bacterial genera Agrobacterium-Rhizobium, Ochrobactrum, Pseudomonas, Anoxybacillus and Penibacillus was recorded over the three years study period. Presence of cytosolic arsenate reductase (arsC) gene was observed within the microbial community. Phylogenetic analyses revealed that all the arsC sequences were closely related to the same gene from γ-proteobacterial members while the community was consisted of mainly α-proteobacterial groups. Such phylogenetic incongruence between 16S rRNA and arsC genes possibly indicated horizontal gene transfer (HGT) of the ars genes within the groundwater community. Overall, the study reported a nearly stable geomicrobial environment and genetic determinant related to As homeostasis gene, and provided a better insight on biogeochemistry of As contaminated aquifer of West Bengal. PMID:26634393

  19. The Geobacillus Pan-Genome: Implications for the Evolution of the Genus (United States)

    Bezuidt, Oliver K.; Pierneef, Rian; Gomri, Amin M.; Adesioye, Fiyin; Makhalanyane, Thulani P.; Kharroub, Karima; Cowan, Don A.


    The genus Geobacillus is comprised of a diverse group of spore-forming Gram-positive thermophilic bacterial species and is well known for both its ecological diversity and as a source of novel thermostable enzymes. Although the mechanisms underlying the thermophilicity of the organism and the thermostability of its macromolecules are reasonably well understood, relatively little is known of the evolutionary mechanisms, which underlie the structural and functional properties of members of this genus. In this study, we have compared 29 Geobacillus genomes, with a specific focus on the elements, which comprise the conserved core and flexible genomes. Based on comparisons of conserved core and flexible genomes, we present evidence of habitat delineation with specific Geobacillus genomes linked to specific niches. Our analysis revealed that Geobacillus and Anoxybacillus share a high proportion of genes. Moreover, the results strongly suggest that horizontal gene transfer is a major factor deriving the evolution of Geobacillus from Bacillus, with genetic contributions from other phylogenetically distant taxa. PMID:27252683

  20. Semi-Field Evaluation of Metofluthrin-Impregnated Nets on Host-Seeking Aedes aegypti and Anopheles dirus. (United States)

    Ponlawat, Alongkot; Kankaew, Prasan; Chanaimongkol, Somporn; Pongsiri, Arissara; Richardson, Jason H; Evans, Brian P


    The efficacy of a metofluthrin-impregnated net (MIN) known as the "Mushikonazu" on the house entry behavior of female Aedes aegypti and Anopheles dirus mosquitoes was evaluated using a semi-field 50-m tunnel setup. While the MIN is labeled for the control of chironomids and moth flies, this study determined the feasibility of using the device, given its current construction and metofluthrin formulation, as a spatial repellent against mosquitoes. Sentinel and cone bioassays were used to determine the insecticidal effect of the MIN. A spatial activity index (SAI) was calculated to evaluate responses of the mosquitoes. For the spatial repellent evaluation against Ae. aegypti, the overall mean of SAI was slightly less than 0 at wk 1 after the MIN application and then decreased for the last 4 wk showing a preference to treatment tent. For An. dirus, the mean SAI at wk 1 was positive, indicating a presumed repellent effect of the MIN against An. dirus. For the subsequent 4 wk, the SAI was negative, indicating a preference for the MIN. Results suggested that the MIN may not be a promising approach to repel Ae. aegypti and An. dirus under field conditions in Thailand. However, it remains probable that the MIN may be effective as a spatial repellent if modifications are made to the metofluthrin concentration or formulation and/or the construction of the device. PMID:27280351

  1. Increasing dietary fiber intake in terms of kiwifruit improves constipation in Chinese patients (United States)

    Chan, Annie On On; Leung, Gigi; Tong, Teresa; Wong, Nina YH


    AIM: To investigate if increased dietary fiber, in terms of kiwifruit, is effective in Chinese constipated patients. METHODS: 33 constipated patients and 20 healthy volunteers were recruited for a 4-wk treatment of kiwi fruit twice daily. Response during wk 1-4 was defined as an increase in complete spontaneous bowl, motion (CSBM) ≥ 1/wk. Secondary efficacy included response during wk 1-4, individual symptoms and scores of bowel habits and constipation. Responses were compared with the baseline run-in period. Colonic transit time and anorectal manometry were performed before and after treatment. RESULTS: Responder rate was 54.5% in the constipated group. The mean CSBM increased after treatment (2.2 ± 2.6 vs 4.4 ± 4.6, P = 0.013). There was also improvement in the scores for bothersomeness of constipation (P = 0.02), and satisfaction of bowel habit (P = 0.001), and decreased in days of laxative used (P = 0.003). There was also improvement in transit time (P = 0.003) and rectal sensation (P < 0.05). However, there was no change in the bowel symptoms or anorectal physiology in the healthy subjects. CONCLUSION: Increasing dietary fiber intake is effective in relieving chronic constipation in Chinese population. PMID:17729399

  2. Computer simulation of liquid-vapor coexistence of confined quantum fluids

    International Nuclear Information System (INIS)

    The liquid-vapor coexistence (LV) of bulk and confined quantum fluids has been studied by Monte Carlo computer simulation for particles interacting via a semiclassical effective pair potential Veff(r) = VLJ + VQ, where VLJ is the Lennard-Jones 12-6 potential (LJ) and VQ is the first-order Wigner-Kirkwood (WK-1) quantum potential, that depends on β = 1/kT and de Boer's quantumness parameter Λ=h/σ√(mε), where k and h are the Boltzmann's and Planck's constants, respectively, m is the particle's mass, T is the temperature of the system, and σ and ε are the LJ potential parameters. The non-conformal properties of the system of particles interacting via the effective pair potential Veff(r) are due to Λ, since the LV phase diagram is modified by varying Λ. We found that the WK-1 system gives an accurate description of the LV coexistence for bulk phases of several quantum fluids, obtained by the Gibbs Ensemble Monte Carlo method (GEMC). Confinement effects were introduced using the Canonical Ensemble (NVT) to simulate quantum fluids contained within parallel hard walls separated by a distance Lp, within the range 2σ ⩽ Lp ⩽ 6σ. The critical temperature of the system is reduced by decreasing Lp and increasing Λ, and the liquid-vapor transition is not longer observed for Lp/σ < 2, in contrast to what has been observed for the classical system

  3. Moderate running and plyometric training during off-season did not show a significant difference on soccer-related high-intensity performances compared with no-training controls. (United States)

    Nakamura, Daisuke; Suzuki, Tomohiro; Yasumatsu, Mikinobu; Akimoto, Takayuki


    Several investigators have reported the effects of reduced training and interrupted training on athletic performance, but few reports are available for soccer players. The purpose of this study was to examine, using the Yo-Yo intermittent recovery level 2 (YoYoIR2) test and sprint performance, the effects on soccer players of a reduced training program consisting of either moderate running training, plyometric training. After the completion of a competitive season, 29 male soccer players were divided into 3 groups: the running group (n = 13), the plyometric group (n = 11), and the control group (n = 5). Both training groups completed either running or plyometric training sessions 2 d·wk(-1) for 3 weeks, whereas the control group was not allowed to perform any training. The subjects performed YoYoIR2 and 20-m sprint tests before (pre) and after (post) the experimental period. Neither training group showed any significant training effects on the YoYoIR2 performance or 20-m sprint times compared with the control group. This study suggests that neither endurance running nor plyometric training 2 d·wk(-1) for 3 weeks has a significant effect on high-intensity performance compared with a nontraining regimen. However, our results do not support complete inactivity. These results may have important implications for the management of training cessation for a few weeks. PMID:22207263

  4. Thermophilic spore-forming bacteria isolated from spoiled canned food and their heat resistance. Results of a French ten-year survey. (United States)

    André, S; Zuber, F; Remize, F


    Thermal processing of Low Acid Canned Foods (LACF), which are safe and shelf-stable at ambient temperature for several years, results in heat inactivation of all vegetative microorganisms and the partial or total inactivation of spores. Good Manufacturing Hygienic Practices include stability tests for managing the pathogen risk related to surviving mesophilic bacterial spores. LACF are also often submitted to additional incubation conditions, typically 55 °C for 7 days, to monitor spoilage by thermophiles. In this study we identified the bacterial species responsible for non-stability after prolonged at 55 °C of incubation of LACF from 455 samples collected from 122 French canneries over 10 years. Bacteria were identified by microsequencing or a recent developed tool for group-specific PCR detection (SporeTraQ™). A single species was identified for 93% of examined samples. Three genera were responsible for more than 80% of all non-stability cases: mostly Moorella (36%) and Geobacillus (35%), and less frequently Thermoanaerobacterium (10%). The other most frequent bacterial genera identified were Bacillus, Thermoanaerobacter, Caldanaerobius, Anoxybacillus, Paenibacillus and Clostridium. Species frequency was dependent on food category, i.e. vegetables, ready-made meals containing meat, seafood or other recipes, products containing fatty duck, and related to the intensity of the thermal treatment applied in these food categories. The spore heat resistance parameters (D or δ and z values) from 36 strains isolated in this study were determined. Taken together, our results single out the species most suitable for use as indicators for thermal process settings. This extensively-documented survey of the species that cause non-stability at 55 °C in LACF will help canneries to improve the management of microbial contamination. PMID:23728430

  5. Potential Application of Anaerobic Extremophiles for Hydrogen Production (United States)

    Pikuta, Elena V.; Hoover, Richard B.


    During substrate fermentation many anaerobes produce the hydrogen as a waste product, which often regulates the growth of the cultures as an inhibitor. In nature the hydrogen is usually removed from the ecosystem due to its physical properties or by consumption of hydrogen by secondary anaerobes, which sometimes behave as competitors for electron donors as is seen in the classical example in anaerobic microbial communities via the interaction between methanogens and sulfate- or sulfur- reducers. It was demonstrated previously on mixed cultures of anaerobes at neutral pH that bacterial hydrogen production could provide an alternative energy source. But at neutral pH the original cultures can easily be contaminated by methanogens, a most unpleasant side effect of these conditions is the development of pathogenic bacteria. In both cases the rate of hydrogen production was dramatically decreased since some part of the hydrogen was transformed to methane, and the cultivation of human pathogens on a global scale is very dangerous. In our laboratory, experiments with obligately alkaliphilic bacteria that excrete hydrogen as the end metabolic product were performed at different temperature regimes. Mesophilic and moderately thermophilic bacterial cultures have been studied and compared for the most effective hydrogen production. For high-mineralized media with pH 9.5-10.0 not many methanogens are known to exist. Furthermore, the development of pathogenic contaminant microorganisms is virtually impossible: carbonate-saturated solutions are used as antiseptics in medicine. Therefore the cultivation of alkaliphilic hydrogen producing bacteria could be considered as most safe process for global Scale industry in future. Here we present experimental data on the rates of hydrogen productivity for mesophilic, alkaliphilic, obligately anaerobic bacterium Spirocheta americana ASpG1 and moderately thermophilic, alkaliphilic, facultative anaerobe Anoxybacillus pushchinoensis K1 and

  6. Spore test parameters matter: Mesophilic and thermophilic spore counts detected in raw milk and dairy powders differ significantly by test method. (United States)

    Kent, D J; Chauhan, K; Boor, K J; Wiedmann, M; Martin, N H


    United States dairy industry exports have steadily risen in importance over the last 10yr, with dairy powders playing a particularly critical role. Currently, approximately half of US-produced nonfat dry milk and skim milk powder is exported. Reaching new and expanding existing export markets relies in part on the control of endospore-forming bacteria in dairy powders. This study reports baseline mesophilic and thermophilic spore counts and spore populations from 55 raw material samples (primarily raw milk) and 33 dairy powder samples from dairy powder processors across the United States. Samples were evaluated using various spore testing methodologies and included initial heat treatments of (1) 80°C for 12 min; (2) 100°C for 30 min; and (3) 106°C for 30 min. Results indicate that significant differences in both the level and population of spores were found for both raw milk and dairy powders with the various testing methods. Additionally, on average, spore counts were not found to increase significantly from the beginning to the end of dairy powder processing, most likely related to the absence of biofilm formation by processing plant-associated sporeformers (e.g., Anoxybacillus sp.) in the facilities sampled. Finally, in agreement with other studies, Bacillus licheniformis was found to be the most prevalent sporeformer in both raw materials and dairy powders, highlighting the importance of this organism in developing strategies for control and reduction of spore counts in dairy powders. Overall, this study emphasizes the need for standardization of spore enumeration methodologies in the dairy powder industry. PMID:27085396

  7. Anterior foregut microbiota of the glassy-winged sharpshooter explored using deep 16S rRNA gene sequencing from individual insects.

    Directory of Open Access Journals (Sweden)

    Elizabeth E Rogers

    Full Text Available The glassy-winged sharpshooter (GWSS is an invasive insect species that transmits Xylella fastidiosa, the bacterium causing Pierce's disease of grapevine and other leaf scorch diseases. X. fastidiosa has been shown to colonize the anterior foregut (cibarium and precibarium of sharpshooters, where it may interact with other naturally-occurring bacterial species. To evaluate such interactions, a comprehensive list of bacterial species associated with the sharpshooter cibarium and precibarium is needed. Here, a survey of microbiota associated with the GWSS anterior foregut was conducted. Ninety-six individual GWSS, 24 from each of 4 locations (Bakersfield, CA; Ojai, CA; Quincy, FL; and a laboratory colony, were characterized for bacteria in dissected sharpshooter cibaria and precibaria by amplification and sequencing of a portion of the 16S rRNA gene using Illumina MiSeq technology. An average of approximately 150,000 sequence reads were obtained per insect. The most common genus detected was Wolbachia; sequencing of the Wolbachia ftsZ gene placed this strain in supergroup B, one of two Wolbachia supergroups most commonly associated with arthropods. X. fastidiosa was detected in all 96 individuals examined. By multilocus sequence typing, both X. fastidiosa subspecies fastidiosa and subspecies sandyi were present in GWSS from California and the colony; only subspecies fastidiosa was detected in GWSS from Florida. In addition to Wolbachia and X. fastidiosa, 23 other bacterial genera were detected at or above an average incidence of 0.1%; these included plant-associated microbes (Methylobacterium, Sphingomonas, Agrobacterium, and Ralstonia and soil- or water-associated microbes (Anoxybacillus, Novosphingobium, Caulobacter, and Luteimonas. Sequences belonging to species of the family Enterobacteriaceae also were detected but it was not possible to assign these to individual genera. Many of these species likely interact with X. fastidiosa in the

  8. Anterior foregut microbiota of the glassy-winged sharpshooter explored using deep 16S rRNA gene sequencing from individual insects. (United States)

    Rogers, Elizabeth E; Backus, Elaine A


    The glassy-winged sharpshooter (GWSS) is an invasive insect species that transmits Xylella fastidiosa, the bacterium causing Pierce's disease of grapevine and other leaf scorch diseases. X. fastidiosa has been shown to colonize the anterior foregut (cibarium and precibarium) of sharpshooters, where it may interact with other naturally-occurring bacterial species. To evaluate such interactions, a comprehensive list of bacterial species associated with the sharpshooter cibarium and precibarium is needed. Here, a survey of microbiota associated with the GWSS anterior foregut was conducted. Ninety-six individual GWSS, 24 from each of 4 locations (Bakersfield, CA; Ojai, CA; Quincy, FL; and a laboratory colony), were characterized for bacteria in dissected sharpshooter cibaria and precibaria by amplification and sequencing of a portion of the 16S rRNA gene using Illumina MiSeq technology. An average of approximately 150,000 sequence reads were obtained per insect. The most common genus detected was Wolbachia; sequencing of the Wolbachia ftsZ gene placed this strain in supergroup B, one of two Wolbachia supergroups most commonly associated with arthropods. X. fastidiosa was detected in all 96 individuals examined. By multilocus sequence typing, both X. fastidiosa subspecies fastidiosa and subspecies sandyi were present in GWSS from California and the colony; only subspecies fastidiosa was detected in GWSS from Florida. In addition to Wolbachia and X. fastidiosa, 23 other bacterial genera were detected at or above an average incidence of 0.1%; these included plant-associated microbes (Methylobacterium, Sphingomonas, Agrobacterium, and Ralstonia) and soil- or water-associated microbes (Anoxybacillus, Novosphingobium, Caulobacter, and Luteimonas). Sequences belonging to species of the family Enterobacteriaceae also were detected but it was not possible to assign these to individual genera. Many of these species likely interact with X. fastidiosa in the cibarium and

  9. The genetic diversity of genus Bacillus and the related genera revealed by 16S rRNA gene sequences and ardra analyses isolated from geothermal regions of turkey

    Directory of Open Access Journals (Sweden)

    Arzu Coleri Cihan


    Full Text Available Previously isolated 115 endospore-forming bacilli were basically grouped according to their temperature requirements for growth: the thermophiles (74%, the facultative thermophiles (14% and the mesophiles (12%. These isolates were taken into 16S rRNA gene sequence analyses, and they were clustered among the 7 genera: Anoxybacillus, Aeribacillus, Bacillus, Brevibacillus, Geobacillus, Paenibacillus, and Thermoactinomycetes. Of these bacilli, only the thirty two isolates belonging to genera Bacillus (16, Brevibacillus (13, Paenibacillus (1 and Thermoactinomycetes (2 were selected and presented in this paper. The comparative sequence analyses revealed that the similarity values were ranged as 91.4-100 %, 91.8- 99.2 %, 92.6- 99.8 % and 90.7 - 99.8 % between the isolates and the related type strains from these four genera, respectively. Twenty nine of them were found to be related with the validly published type strains. The most abundant species was B. thermoruber with 9 isolates followed by B. pumilus (6, B. lichenformis (3, B. subtilis (3, B. agri (3, B. smithii (2, T. vulgaris (2 and finally P. barengoltzii (1. In addition, isolates of A391a, B51a and D295 were proposed as novel species as their 16S rRNA gene sequences displayed similarities ≤ 97% to their closely related type strains. The AluI-, HaeIII- and TaqI-ARDRA results were in congruence with the 16S rRNA gene sequence analyses. The ARDRA results allowed us to differentiate these isolates, and their discriminative restriction fragments were able to be determined. Some of their phenotypic characters and their amylase, chitinase and protease production were also studied and biotechnologically valuable enzyme producing isolates were introduced in order to use in further studies.

  10. Making soy sauce from defatted soybean meal without the mejus process by submerged cultivation using thermophilic bacteria. (United States)

    Hur, Jeong Min; Park, Doo Hyun


    The diversity of thermophilic bacteria was not significantly altered while growing in a defatted soybean meal (DFSM) slurry at 60 °C for 10, 20, and 30 days. Five species of thermophilic bacteria, which belong to the genera Aeribacillus (temperature gradient gel electrophoresis [TGGE] band no. 1), Saccharococcus (TGGE band no. 2), Geobacillus (TGGE band no. 3), Bacillus (TGGE band no. 4), and Anoxybacillus (TGGE band no. 5), were detected in the fermenting DFSM slurry. The cell-free culture fluid obtained from the fermenting DFSM slurry on day 14 hydrolyzed starch and soy protein at 60 °C but not at 30 °C. Soy sauce (test soy sauce) was prepared from the fermented DFSM slurry after a 30 day cultivation at 60 °C and a 60 day ripening at 45 °C. Free amino acid (AA) and organic acid contents in the soy sauce increased in proportion to the fermentation period, whereas ammonium decreased proportionally. Mg and Ca contained in the soy sauce decreased proportionally during fermentation and were lower than those in the non-fermented DFSM extract (control). Spectral absorbance of soy sauce prepared from the fermented DFSM slurry was maximal at 430 nm and increased slightly in proportion to the fermentation period. The aroma and flavor of the test soy sauce were significantly different from those of traditional Korean soy sauce. Conclusively, soy sauce may be prepared directly from the fermented DFSM slurry without meju-preparing process and fermentation period may be a factor for control of soy sauce quality. PMID:26243923

  11. A randomized, double-blind, placebo-controlled trial assessing the efficacy and safety of tegaserod in patients from China with chronic constipation

    Institute of Scientific and Technical Information of China (English)

    San-Ren Lin; Mei-Yun Ke; Jin-Yan Luo; Yao-Zong Yuan; Ji-Yao Wang; Shelley diTommaso; Verena Walter; Jiaqing Huang


    AIM: To evaluate the efficacy and safety of tegaserod,6 mg twice daily (b.i.d.), in men and women with chronic constipation (CC) from China.METHODS: This was a multicenter, double-blind,placebo-controlled study. Following a 2-wk treatmentfree baseline period, patients were randomized to receive either tegaserod (6 mg b.i.d.) or placebo (b.i.d.) for 4 wk. An analysis of covariance with repeated measures was used to determine the overall effect of treatment for the primary efficacy variable; the change from baseline in the number of complete spontaneous bowel movements (CSBMs) during the 4-wk treatment period.Secondary efficacy endpoints included other measures of response in terms of CSBMs, and patients' daily and weekly assessment of bowel habits. Safety was also assessed, based on the incidence and severity of adverse events (AEs).RESULTS: A total of 607 patients were randomized to receive either tegaserod (n = 304) or placebo (n = 303).Tegaserod treatment resulted in a rapid and significant increase from baseline in the adjusted mean number of CSBMs per week over wk 1-4 compared with placebo(1.39 vs 0.91, P = 0.0002). A statistically significant difference in favor of tegaserod was also observed for a mean increase ≥ 1 CSBM/wk over wk 1-4 (47.7%vs 35.0%, tegaserod vs placebo, respectively, P =0.0018) and for the absolute number of ≥ 3 CSBMs/wkover wk 1-4 (25.0% vs 14.5%, tegaserod vs placebo,respectively, P = 0.0021). Improvements in other symptoms of CC were also seen in the tegaserod group,including improved stool form and reduced straining. In addition, more patients in the tegaserod group reported satisfactory relief from their constipation symptoms. The frequency and severity of AEs was comparable between tegaserod and placebo groups, with the exception of a greater incidence of diarrhea in patients receiving tegaserod (3.6%) compared with placebo (1.7%).CONCLUSION: Tegaserod treatment improved multiple symptoms of CC and was associated with a favorable

  12. Getting out and about in older adults: the nature of daily trips and their association with objectively assessed physical activity

    Directory of Open Access Journals (Sweden)

    Sharp Debbie J


    Full Text Available Abstract Background A key public health objective is increasing health-enhancing physical activity (PA for older adults (OAs. Daily trip frequency is independently associated with objectively assessed PA volumes (OAs. Little is known about correlates and these trips' transport mode, and how these elements relate to PA. Purpose: to describe the frequency, purpose, and travel mode of daily trips in OAs, and their association with participant characteristics and objectively-assessed PA. Methods Participants (n = 214, aged 78.1 SD 5.7 years, completed a seven-day trips log recording daily-trip frequency, purpose and transport mode. Concurrently participants wore an accelerometer which provided mean daily steps (steps·d-1, and minutes of moderate to vigorous PA (MVPA·d-1. Participants' physical function (PF was estimated and demographic, height and weight data obtained. Results Trip frequency was associated with gender, age, physical function, walking-aid use, educational attainment, number of amenities within walking distance and cars in the household. Participants reported 9.6 (SD 4.2 trips per week (trips·wk-1. Most trips (61% were by car (driver 44%, passenger 17%, 30% walking or cycling (active and 9% public transport/other. Driving trips·wk-1 were more common in participants who were males (5.3 SD 3.6, well-educated (5.0 SD 4.3, high functioning (5.1 SD 4.6, younger (5.6 SD 4.9, affluent area residents (5.1 SD 4.2 and accessing > one car (7.2 SD 4.7. Active trips·wk-1 were more frequent in participants who were males (3.4 SD 3.6, normal weight (3.2 SD 3.4, not requiring walking aids (3.5 SD 3.3, well-educated (3.7 SD 0.7, from less deprived neighbourhoods (3.9 SD 3.9 and with ≥ 8 amenities nearby (4.4 SD 3.8. Public transport, and active trip frequency, were significantly associated with steps·d-1 (p -1 (p Conclusions Daily trips are associated with objectively-measured PA as indicated by daily MVPA and steps. Public transport and

  13. Spontaneous Migration of Polyethylene Molecule Sheathed inside Single-Walled Carbon Nanotube for Nano-Heat Pipe. (United States)

    Liao, Quanwen; Liu, Zhichun; Yang, Nuo; Liu, Wei


    Development of nanoscale thermal exchanging devices is critical to thermal management in nanoscale. The spontaneous migration of polyethylene molecule sheathed inside single-walled carbon nanotube (SWCNT) are observed. And the multi-factor analysis of spontaneous migration about temperature, mass and potential barrier shows new features about motion mechanisms, and enriches the existing mass transport theory greatly. Here, based on this finding, we report a nano-heat pipe (NHP) composing of a SWCNT and a polyethylene molecule. Using molecular dynamics simulations, the heat exchanging coefficient can reach 450 WK(-1) cm(-2) at 500 K by NHP arrays with a quantity density of 7 × 10(13) cm(-2). This study will benefit the designs of NHP and other nanoscale mass transport devices. PMID:27211247

  14. Impact of hyperketonemia in early lactation dairy cows on health and production. (United States)

    Duffield, T F; Lissemore, K D; McBride, B W; Leslie, K E


    Data from 1,010 lactating lactating, predominately component-fed Holstein cattle from 25 predominately tie-stall dairy farms in southwest Ontario were used to identify objective thresholds for defining hyperketonemia in lactating dairy cattle based on negative impacts on cow health, milk production, or both. Serum samples obtained during wk 1 and 2 postpartum and analyzed for beta-hydroxybutyrate (BHBA) concentrations that were used in analysis. Data were time-ordered so that the serum samples were obtained at least 1 d before the disease or milk recording events. Serum BHBA cutpoints were constructed at 200 micromol/L intervals between 600 and 2,000 micromol/L. Critical cutpoints for the health analysis were determined based on the threshold having the greatest sum of sensitivity and specificity for predicting the disease occurrence. For the production outcomes, models for first test day milk yield, milk fat, and milk protein percentage were constructed including covariates of parity, precalving body condition score, season of calving, test day linear score, and the random effect of herd. Each cutpoint was tested in these models to determine the threshold with the greatest impact and least risk of a type 1 error. Serum BHBA concentrations at or above 1,200 micromol/L in the first week following calving were associated with increased risks of subsequent displaced abomasum [odds ratio (OR) = 2.60] and metritis (OR = 3.35), whereas the critical threshold of BHBA in wk 2 postpartum on the risk of abomasal displacement was >or=1,800 micromol/L (OR = 6.22). The best threshold for predicting subsequent risk of clinical ketosis from serum obtained during wk 1 and wk 2 postpartum was 1,400 micromol/L of BHBA (OR = 4.25 and 5.98, respectively). There was no association between clinical mastitis and elevated serum BHBA in wk 1 or 2 postpartum, and there was no association between wk 2 BHBA and risk of metritis. Greater serum BHBA measured during the first and second week

  15. The grand partition function Z (α,β) of a quantum system is studied, using diagrammatic representations of the perturbation expansion

    International Nuclear Information System (INIS)

    The grand partition function Z (α,β) of a quantum system is studied, using diagrammatic representations of the perturbation expansion. For a fermions system, it is possible to show, by proper resummation, without approximations but under some 'regularity hypothesis', that Log Z (α,β) takes a form where, besides trivial dependences, α and β only appear through a statistical factor Fk- = [1 + e-α+βεk0-βWk]-1. Wk is a (real) self-consistent potential, generalized to all orders and can be defined by a stationary condition on Log Z (α,β) under variations of Fk-. The thermodynamical quantities take a form analogous to the expressions Landau introduced for the Fermi liquids. The zero temperature limit (for isotropic systems) gives back Goldstone expressions for the ground state of a system. (author)

  16. Urns with simultaneous drawing

    CERN Document Server

    Launay, Mickaël


    In classical urn models, one usually draws one ball with replacement at each time unit and then adds one ball of the same colour. Given a weight sequence $(w_k)_{k\\in\\N}$, the probability of drawing a ball of a certain colour is proportional to $w_k$ where $k$ is the number of balls of this colour. A classical result states that an urn fixates on one colour after a finite time if an only if $\\sum_{0}^\\infty w_k^{-1} < \\infty$. In this paper we shall study the case when at each time unit we draw with replacement a number $d\\in\\N$ of balls and then add $d$ new balls of matching colours. The main goal is to prove that the result in the case of maximal interaction generalizes assuming in addition that $(w_k)_{k\\in\\N}$ is non-decreasing.

  17. Effect of training in the fasted state on metabolic responses during exercise with carbohydrate intake

    DEFF Research Database (Denmark)

    De Bock, K; Derave, W; Eijnde, B O;


    program (6 wk, 3 day/wk, 1-2 h, 75% of peak Vo(2)) in moderately active males. They trained in the fasted (F; n = 10) or carbohydrate-fed state (CHO; n = 10) while receiving a standardized diet [65 percent of total energy intake (En) from carbohydrates, 20%En fat, 15%En protein]. Before and after the...... training period, substrate use during a 2-h exercise bout was determined. During these experimental sessions, all subjects were in a fed condition and received extra carbohydrates (1 body wt(-1) .h(-1)). Peak Vo(2) (+7%), succinate dehydrogenase activity, GLUT4, and hexokinase II content were...... elicits similar adaptations in peak Vo(2) whether carried out in the fasted or carbohydrate-fed state. Although there was a decrease in exercise-induced glycogen breakdown and an increase in proteins involved in fat handling after fasting training, fat oxidation during exercise with carbohydrate intake...

  18. The Effects of Dietary Phosphorus on the Growth Performance and Phosphorus Excretion of Dairy Heifers. (United States)

    Zhang, B; Wang, C; Wei, Z H; Sun, H Z; Xu, G Z; Liu, J X; Liu, H Y


    The objective of this study was to investigate the effects of reducing dietary phosphorus (P) on the frame size, udder traits, blood parameters and nutrient digestibility coefficient in 8- to 10-month-old Holstein heifers. Forty-five heifers were divided into 15 blocks according to the mo of age and were randomly assigned one of three dietary treatments: 0.26% (low P [LP]), 0.36% (medium P [MP]), or 0.42% (high P [HP]) (dry matter basis). Samples were collected at the wk 1, 4, 8. The results show that low dietary P had no effect on body measurement. The blood P concentration decreased with decreasing dietary P (penvironment. PMID:26954160

  19. Amounts of substrate carbon and nitrogen control the decomposition of soil organic matter (United States)

    Liu, X. J. A.; Sun, J.; Finley, B. K.; Dijkstra, P.; Schwartz, E.; Hungate, B. A.


    Climate change, mainly caused by rising atmospheric CO2 and nitrogen (N) deposition due to human activities, is postulated to increase energy and nutrient inputs to soils that can accelerate or retard soil organic matter (SOM) decomposition, a phenomenon called the priming effect. Yet, the direction and magnitude of priming in response to different amounts of energy and nutrient inputs remain elusive. Here we examined the effects of carbon (C) and N additions on priming, microbial turnover, extracellular enzyme activities, CO2 fluxes, and C accumulation in four different ecosystems. We applied low and high C (13C glucose; 350 and 1000 μg C g-1 wk-1) and C with N (NH4NO3; 35 and 100 μg N g-1 wk-1) for five weeks. We found: 1) high C and high C+N stimulated weekly priming in the first two weeks and then leveled off, indicating soil microorganisms may have a short-term of accelerated growth and activity but quickly adapt to frequent inputs of high substrate amounts, 2) high C induced greater cumulative priming, microbial turnover, and β-glucosidase activities than low C, 3) high C+N had significantly lower cumulative priming, turnover, and β-glucosidase activities than high C, 4) high C and high C+N stimulated greater CO2 fluxes and C accumulations than low substrate inputs. These results suggest that the amount of substrate (energy and nutrient) was a determinant factor in modulating the rate of SOM decomposition, microbial turnover, enzyme activities, and C balance. Overall we demonstrate that increased energy inputs can quickly accelerate SOM decomposition, but concurrent nutrient inputs can suppress such process, which could have a huge impact on terrestrial C storage and global biogeochemical C cycling under climate change.

  20. Quantification of milk yield and composition changes as affected by subclinical mastitis during the current lactation in sheep. (United States)

    Martí De Olives, Ana; Díaz, J R; Molina, M P; Peris, C


    The aim of this work was to quantify, on a half-udder basis, the changes in ewe milk yield and composition caused by unilateral subclinical mastitis within the current lactation. Fluctuations due to production level, infection severity, time from the onset of infection, and lactation curves were also studied. Yield and composition of milk from half-udders of unilateral infected ewes were compared between them and with a set of healthy halves using a mixed model. The experiment was completed with a whole-udder approach on the same animals. To test the effect of intramammary infection (IMI) in the 7 wk following the onset of infection, 20 ewes that acquired unilateral subclinical mastitis during lactation and 40 healthy ewes were used. Another group of 20 unilaterally infected ewes from wk 1 of lactation and other 40 healthy ewes were studied to test the effect of IMI on lactational milk yield and composition. The individual milk loss in ewes infected during lactation was 15% for the 7 wk following the onset of infection, and 6.6% more milk was produced by the uninfected half to compensate milk lost by the infected half. Lactational milk yield loss in ewes infected from wk 1 postpartum was 17%. The changes in milk yield were noticed from the week of infection diagnosis. The production level of animals influenced the milk yield changes caused by IMI in such a way that the more productive ewes lost more milk, although these losses were proportional to their production level. On the other hand, infection severity affected milk loss between glands, being more pronounced as somatic cell count increased. A clear decrease of lactose content and casein:protein ratio due to subclinical IMI was observed and it remained throughout the postinfection period. Improving udder health status is necessary to maintain milk production and quality in dairy ewes during lactation. PMID:24119805

  1. The effect of grape seed and grape marc meal extract on milk performance and the expression of genes of endoplasmic reticulum stress and inflammation in the liver of dairy cows in early lactation. (United States)

    Gessner, D K; Koch, C; Romberg, F-J; Winkler, A; Dusel, G; Herzog, E; Most, E; Eder, K


    During the periparturient phase, cows are typically in an inflammation-like condition, and it has been suggested that inflammation associated with the development of stress of the endoplasmic reticulum (ER) in the liver contributes to the development of fatty liver syndrome and ketosis. In the present study, we investigated the hypothesis that feeding grape seed and grape marc meal extract (GSGME) as a plant extract rich in flavonoids attenuates inflammation and ER stress in the liver of dairy cows. Two groups of cows received either a total mixed ration as a control diet or the same total mixed ration supplemented with 1% of GSGME over the period from wk 3 prepartum to wk 9 postpartum. Dry matter intake during wk 3 to 9 postpartum was not different between the 2 groups. However, the cows fed the diet supplemented with GSGME had an increased milk yield and an increased daily milk protein yield. Cows supplemented with GSGME moreover had a significantly reduced mRNA abundancy of fibroblast growth factor (FGF) 21, a stress hormone induced by various stress conditions, in the liver in wk 1 and 3 postpartum. In contrast, mRNA abundances of a total of 3 genes involved in inflammation and 14 genes involved in ER stress response, as well as concentrations of triacylglycerols and cholesterol, in liver samples of wk 1 and 3 postpartum did not differ between the 2 groups. Overall, this study shows that supplementation of GSGME did not influence inflammation or ER stress in the liver but increased milk yield, an effect that could be due to effects on ruminal metabolism. PMID:26409958

  2. Prospective study of physical activity and risk of squamous cell carcinoma of the skin

    International Nuclear Information System (INIS)

    The relationship between physical activity and risk of cutaneous squamous cell carcinoma (SCC) is unknown and difficult to investigate due to confounding by sun exposure. We prospectively examined the association of recreational and occupational physical activity and incidence of SCC accounting for photoaging and other risk factors. We used available information on physical activity from the Australian population-based Nambour Skin Cancer Study comprising 1,171 adults aged 25-75 years at baseline (1992). In sex-stratified analyses (person-based and tumor-based) we estimated the associations between type of activity and incidence of SCC prospectively to 2007. During 16 years of follow-up, 98 men and 90 women newly developed SCC. We found no significant association between recreational activity measures and SCC after controlling for potential confounding factors including indicators of sun exposure. In men, the observed risk pattern was however suggestive of elevated risk with increasing total hours of recreational activity (compared to inactive men, RR (95%CI) 0.89 (0.54, 1.46) for ≤ 1.5 hrs/wk; 1.29 (0.82, 2.04) for ≤ 4.0 hrs/wk; 1.33 (0.86, 2.05) > 4.0 hrs/wk), while among women, higher level of occupational activity (standing and manual versus sedentary work activities) was associated with a reduced incidence of SCC tumors (P trend = 0.03). Despite some suggestion that recreational activity in men and occupational activity in women are related to occurrence of SCC, there is no firm support for a role of physical activity in the development of cutaneous SCC

  3. Prospective study of physical activity and risk of squamous cell carcinoma of the skin

    Directory of Open Access Journals (Sweden)

    Lahmann Petra H


    Full Text Available Abstract Background The relationship between physical activity and risk of cutaneous squamous cell carcinoma (SCC is unknown and difficult to investigate due to confounding by sun exposure. We prospectively examined the association of recreational and occupational physical activity and incidence of SCC accounting for photoaging and other risk factors. Methods We used available information on physical activity from the Australian population-based Nambour Skin Cancer Study comprising 1,171 adults aged 25-75 years at baseline (1992. In sex-stratified analyses (person-based and tumor-based we estimated the associations between type of activity and incidence of SCC prospectively to 2007. Results During 16 years of follow-up, 98 men and 90 women newly developed SCC. We found no significant association between recreational activity measures and SCC after controlling for potential confounding factors including indicators of sun exposure. In men, the observed risk pattern was however suggestive of elevated risk with increasing total hours of recreational activity (compared to inactive men, RR (95%CI 0.89 (0.54, 1.46 for ≤ 1.5 hrs/wk; 1.29 (0.82, 2.04 for ≤ 4.0 hrs/wk; 1.33 (0.86, 2.05 > 4.0 hrs/wk, while among women, higher level of occupational activity (standing and manual versus sedentary work activities was associated with a reduced incidence of SCC tumors (P trend = 0.03. Conclusions Despite some suggestion that recreational activity in men and occupational activity in women are related to occurrence of SCC, there is no firm support for a role of physical activity in the development of cutaneous SCC.

  4. The effect of northern fowl mite (Ornithonyssus sylviarum) infestation on hen physiology, physical condition, and egg quality. (United States)

    Vezzoli, Giuseppe; King, Annie J; Mench, Joy A


    The northern fowl mite (NFM),Ornithonyssus sylviarum, is the most common ectoparasite of laying hens in North America. Infestation can cause a reduction in egg production, egg weights, and feed conversion efficiency. However, there is a lack of information on the effects of NFM on hen physiology, physical condition, and egg quality. Singly caged beak-trimmed White Leghorn hens (N=32) were infested with mites at 25 wk of age. The condition of each hen was assessed at wk 0 (infestation) and wk 5 and 7 post-infestation to determine comb temperatures and feather, skin, and comb condition. Heterophil-lymphocyte (H/L) ratios and body weight (BW) were evaluated at wk 0 and wk 1, 3, 5, and 7 post-infestation. Egg weight, egg specific gravity, yolk color, Haugh unit (HU), and eggshell thickness were determined prior to infestation (wk -1) and at 1, 3, 5, and 7 wk post-infestation. The H/L ratio (Phens had red skin and more than 75% had scabs on the vent; in addition more than 84% had grey-black vent feathers. There were no effects of infestation on comb color, comb temperature, feather cover, BW, or eggshell thickness. It was concluded that infestation with NFM has negative effects on interior egg quality and hen integument. A decrease in H/L ratio was also observed at the peak of infestation. However, the effects of NFM on the immune system are unclear, and H/L ratio might not be a good stress measure in hens highly infested with NFM. PMID:26944982

  5. Epigenetic regulation of pyruvate carboxylase gene expression in the postpartum liver. (United States)

    Walker, C G; Crookenden, M A; Henty, K M; Handley, R R; Kuhn-Sherlock, B; White, H M; Donkin, S S; Snell, R G; Meier, S; Heiser, A; Loor, J J; Mitchell, M D; Roche, J R


    Hepatic gluconeogenesis is essential for maintenance of whole body glucose homeostasis and glucose supply for mammary lactose synthesis in the dairy cow. Upregulation of the gluconeogenic enzyme pyruvate carboxylase (PC) during the transition period is vital in the adaptation to the greater glucose demands associated with peripartum lactogenesis. The objective of this study was to determine if PC transcription in hepatocytes is regulated by DNA methylation and if treatment with a nonsteroidal anti-inflammatory drug (NSAID) alters methylation of an upstream DNA sequence defined as promoter 1. Dairy cows were left untreated (n=20), or treated with a NSAID during the first 5 d postcalving (n=20). Liver was biopsied at d 7 precalving and d 7, 14, and 28 postcalving. Total PC and transcript specific gene expression was quantified using quantitative PCR and DNA methylation of promoter 1 was quantified using bisulfite Sanger sequencing. Expression of PC changed over the transition period, with increased expression postcalving occurring concurrently with increased circulating concentration of nonesterified fatty acids. The DNA methylation percentage was variable at all sites quantified and ranged from 21 to 54% across the 15 CpG dinucleotides within promoter 1. The DNA methylation at wk 1 postcalving, however, was not correlated with gene expression of promoter 1-regulated transcripts and we did not detect an effect of NSAID treatment on DNA methylation or PC gene expression. Our results do not support a role for DNA methylation in regulating promoter 1-driven gene expression of PC at wk 1 postcalving. Further research is required to determine the mechanisms regulating increased PC expression over the transition period. PMID:27085418

  6. Search for the algorithm of genes distribution during the process of microbial evolution (United States)

    Pikuta, Elena V.


    Previous two and three dimensional graph analysis of eco-physiological data of Archaea demonstrated specific geometry for distribution of major Prokaryotic groups in a hyperboloid function. The function of a two-sheet hyperboloid covered all known biological groups, and therefore, could be applied for the entire evolution of life on Earth. The vector of evolution was indicated from the point of hyper temperature, extreme acidity and low salinity to the point of low temperature and increased alkalinity and salinity. According to this vector, the following groups were chosen for the gene screening analysis. In the vector "High-Temperature → Low-Temperature" within extreme acidic pH (0-3), it is: 1) the hyperthermophilic Crenarchaeota - order Sulfolobales, 2) moderately thermophilic Euryarchaeota - Class Thermoplasmata, and 3) mesophilic acidophiles- genus Thiobacillus and others. In the vector "Low pH → High pH" the following groups were selected in three temperature ranges: a) Hyperthermophilic Archaea and Eubacteria, b) moderately thermophilic - representatives of the genera Anaerobacter and Anoxybacillus, and c) mesophilic haloalkaliphiles (Eubacteria and Archaea). The genes associated with acidophily (H+ pump), chemolitho-autotrophy (proteins of biochemichal cycles), polymerases, and histones were proposed for the first vector, and for the second vector the genes associated with halo-alkaliphily (Na+ pumps), enzymes of organotrophic metabolisms (sugar- and proteolytics), and others were indicated for the screening. Here, an introduction to the phylogenetic constant (ρη) is presented and discussed. This universal characteristic is calculated for two principally different life forms -Prokaryotes and Eukaryotes; Existence of the second type of living forms is impossible without the first one. The number of chromosomes in Prokaryotic organisms is limited to one (with very rare exceptions, to two), while in Eukaryotic organisms this number is larger. Currently

  7. Piezophilic Bacteria Isolated from Sediment of the Shimokita Coalbed, Japan (United States)

    Fang, J.; Kato, C.; Hori, T.; Morono, Y.; Inagaki, F.


    RNA gene sequences, we have identified abundant Alkalibacterium sp. in 6R-3 and 29R-7 at the first HP cultivation. We also identified Haloactibacillus sp. in 6R-3 and Anoxybacillus related sp. in 19R-1~25R-3 at the third HP cultivation. These microorganisms are likely piezophiles and play an important role in degradation of sedimentary organic matter and production of microbial metabolites sustaining the deep microbial ecosystem in the Shimokita Coalbed. The complete 16S sequencing and isolation of piezophiles are now ongoing.

  8. Toward improved postpartum cyclicity of primiparous dairy cows: Effects of genetic merit for production traits under contrasting feeding systems. (United States)

    Bedere, N; Delaby, L; Ducrocq, V; Leurent-Colette, S; Disenhaus, C


    Milk genetic merit is known to affect commencement of luteal activity (C-LA) in dairy cows. This effect is considered to be due to energy exported in milk production. The present study aimed to identify and quantify the effects of genetic characteristics [breed and estimated breeding value (EBV) for milk yield and fat and protein contents] and feeding system on C-LA of primiparous cows. From 2006 to 2013, an experiment was conducted on 97 primiparous dairy (Holstein) and 97 primiparous dual-purpose (Normande) cows. Within breed, cows were classified into 2 groups: cows with high EBV for milk yield were included in a "milk group" and those with high EBV for fat and protein contents were included in a "content group." Within breed, exported energy in milk and body weight (BW) loss were similar for both genetic groups. Two grazing-based strategies were used, a high feeding system (maize silage in winter and grazing plus concentrate) and a low feeding system (grass silage in winter and grazing with no concentrate). Interval from calving to C-LA was studied performing survival analyses. Milk progesterone profile, milk yield, and body condition were analyzed using χ(2)-test and analysis of covariance. Holstein cows produced more milk (+1,810 kg in the high feeding system and +1,120 kg in the low feeding system) and lost more BW from wk 1 to 14 of lactation (-1.4 kg/wk) than Normande cows, whereas Normande cows had earlier C-LA than Holstein cows. Within breed, cows in the content group had earlier C-LA (associated hazard ratio=2.0) than cows in the milk group. Body weight at calving and loss from wk 1 to 14 of lactation tended to be associated with later C-LA. Cows in the high feeding system produced more milk (+2,040 kg for the Holstein cows and +1,350 kg for Normande cows) and lost less BW from wk 1 to 14 of lactation (+3.8 kg/wk) than cows in the low feeding system. No effect of feeding system or milk yield was observed on C-LA. Prolonged luteal phases were frequent

  9. Comprehensive Population-Averaged Arterial Input Function for Dynamic Contrast–Enhanced vMagnetic Resonance Imaging of Head and Neck Cancer

    Energy Technology Data Exchange (ETDEWEB)

    Onxley, Jennifer D.; Yoo, David S. [Department of Radiation Oncology, Duke University Medical Center, Durham, North Carolina (United States); Muradyan, Naira [iCAD Inc., Nashua, New Hampshire (United States); MacFall, James R. [Department of Radiology, Duke University Medical Center, Durham, North Carolina (United States); Brizel, David M. [Department of Radiation Oncology, Duke University Medical Center, Durham, North Carolina (United States); Department of Surgery, Duke University Medical Center, Durham, North Carolina (United States); Craciunescu, Oana I., E-mail: [Department of Radiation Oncology, Duke University Medical Center, Durham, North Carolina (United States)


    Purpose: To generate a population-averaged arterial input function (PA-AIF) for quantitative analysis of dynamic contrast-enhanced MRI data in head and neck cancer patients. Methods and Materials: Twenty patients underwent dynamic contrast-enhanced MRI during concurrent chemoradiation therapy. Imaging consisted of 2 baseline scans 1 week apart (B1/B2) and 1 scan after 1 week of chemoradiation therapy (Wk1). Regions of interest (ROIs) in the right and left carotid arteries were drawn on coronal images. Plasma concentration curves of all ROIs were averaged and fit to a biexponential decay function to obtain the final PA-AIF (AvgAll). Right-sided and left-sided ROI plasma concentration curves were averaged separately to obtain side-specific AIFs (AvgRight/AvgLeft). Regions of interest were divided by time point to obtain time-point-specific AIFs (AvgB1/AvgB2/AvgWk1). The vascular transfer constant (K{sub trans}) and the fractional extravascular, extracellular space volume (V{sub e}) for primaries and nodes were calculated using the AvgAll AIF, the appropriate side-specific AIF, and the appropriate time-point-specific AIF. Median K{sub trans} and V{sub e} values derived from AvgAll were compared with those obtained from the side-specific and time-point-specific AIFs. The effect of using individual AIFs was also investigated. Results: The plasma parameters for AvgAll were a{sub 1,2} = 27.11/17.65 kg/L, m{sub 1,2} = 11.75/0.21 min{sup −1}. The coefficients of repeatability (CRs) for AvgAll versus AvgLeft were 0.04 min{sup −1} for K{sub trans} and 0.02 for V{sub e}. For AvgAll versus AvgRight, the CRs were 0.08 min{sup −1} for K{sub trans} and 0.02 for V{sub e}. When AvgAll was compared with AvgB1/AvgB2/AvgWk1, the CRs were slightly higher: 0.32/0.19/0.78 min{sup −1}, respectively, for K{sub trans}; and 0.07/0.08/0.09 for V{sub e}. Use of a PA-AIF was not significantly different from use of individual AIFs. Conclusion: A PA-AIF for head and neck cancer

  10. The effects of a ration change from a total mixed ration to pasture on rumen fermentation, volatile fatty acid absorption characteristics, and morphology of dairy cows. (United States)

    Schären, M; Seyfang, G M; Steingass, H; Dieho, K; Dijkstra, J; Hüther, L; Frahm, J; Beineke, A; von Soosten, D; Meyer, U; Breves, G; Dänicke, S


    To investigate the effect of the change from a concentrate and silage-based ration (total mixed ration, TMR) to a pasture-based ration, a 10-wk trial (wk 1-10) was performed, including 10 rumen- and duodenum-fistulated German Holstein dairy cows (182±24 d in milk, 23.5±3.5kg of milk/d; mean ± standard deviation). The cows were divided in either a pasture group (PG, n=5) or a confinement group (CG, n=5). The CG stayed on a TMR-based ration (35% corn silage, 35% grass silage, 30% concentrate; dry matter basis), whereas the PG was gradually transitioned from a TMR to a pasture-based ration (wk 1: TMR only; wk 2: 3 h/d on pasture wk 3 and 4: 12 h/d on pasture wk 5-10: pasture only). Ruminal pH, volatile fatty acids (VFA), NH3-N, and lipopolysaccharide (LPS) concentrations were measured in rumen fluid samples collected medially and ventrally on a weekly basis. Ruminal pH was continuously recorded during 1 to 4 consecutive days each week using ruminal pH measuring devices. In wk 1, 5, and 10, rumen contents were evacuated and weighed, papillae were collected from 3 locations in the rumen, and subsequently a VFA absorption test was performed. In the PG, mean rumen pH and molar acetate proportions decreased, and molar butyrate proportions increased continuously over the course of the trial, which can most likely be ascribed to an increased intake of rapidly fermentable carbohydrates. During the first weeks on a full grazing ration (wk 5-7), variation of rumen pH decreased, and in wk 5 a lower rumen content, papillae surface area, and potential for VFA absorption were observed. In wk 8 to 10, variation of rumen pH and total VFA concentrations increased again, and acetate/propionate ratio decreased. In wk-10 rumen content, papillae area and VFA absorption characteristics similar to initial levels were observed. Although continuous rumen pH assessments and LPS concentrations did not reveal an increased risk for subacute rumen acidosis (SARA) during the adaption period

  11. Repeatability of metabolic responses to a nutrient deficiency in early and mid lactation and implications for robustness of dairy cows. (United States)

    Gross, J J; Bruckmaier, R M


    Nutrient partitioning toward the mammary gland during insufficient energy and nutrient supply is a strategy to ensure survival of the offspring in mammalian species. This homeorhetic priority of the mammary gland is also present in the modern dairy cow, in particular in early lactation. However, despite similar metabolic loads, the adaptive response to a given metabolic load varies considerably among animals. The aim of this study was to investigate if individual cows respond in a consistent manner to a negative energy balance (NEB) in early and mid lactation. Twenty-five dairy cows experienced the usual NEB after parturition and were subjected to a second 3-wk NEB induced by feed restriction in mid lactation. Animals were retrospectively ranked according to their highest plasma nonesterified fatty acid (NEFA) concentration in wk 1 to 4 postpartum. The animals with the 33% highest and 33% lowest values were selected and classified either as the high response (HR) or low response (LR) group. Before parturition, no differences in the studied parameters, dry matter intake, energy balance, concentrations of glucose, NEFA, β-hydroxybutyrate, cholesterol, triglycerides, growth hormone, and insulin-like growth factor-1, were detected between LR and HR. After parturition, milk yield and energy-corrected milk yield was higher for HR compared with LR in wk 2 to 14 and wk 1 to 6, respectively. During feed restriction in wk 15 to 17 postpartum, no differences in energy-corrected milk between LR and HR were found. Energy balance was more negative in HR during the NEB in early lactation, but not different from LR during feed restriction in mid lactation. Although plasma concentrations of glucose, growth hormone, triglycerides, and cholesterol showed group differences in early lactation, but not during feed restriction, the plasma concentrations of NEFA, β-hydroxybutyrate, and insulin-like growth factor-1 in HR changed repeatedly to a greater extent during the NEB at the 2

  12. Short communication: Proteins from circulating exosomes represent metabolic state in transition dairy cows. (United States)

    Crookenden, M A; Walker, C G; Peiris, H; Koh, Y; Heiser, A; Loor, J J; Moyes, K M; Murray, A; Dukkipati, V S R; Kay, J K; Meier, S; Roche, J R; Mitchell, M D


    Biomarkers that identify prepathological disease could enhance preventive management, improve animal health and productivity, and reduce costs. Circulating extracellular vesicles, particularly exosomes, are considered to be long-distance, intercellular communication systems in human medicine. Exosomes provide tissue-specific messages of functional state and can alter the cellular activity of recipient tissues through their protein and microRNA content. We hypothesized that exosomes circulating in the blood of cows during early lactation would contain proteins representative of the metabolic state of important tissues, such as liver, which play integral roles in regulating the physiology of cows postpartum. From a total of 150 cows of known metabolic phenotype, 10 cows were selected with high (n=5; high risk) and low (n=5; low risk) concentrations of nonesterified fatty acids, β-hydroxybutyrate, and liver triacylglycerol during wk 1 and 2 after calving. Exosomes were extracted from blood on the day of calving (d 0) and postcalving at wk 1 and wk 4, and their protein composition was determined by mass spectroscopy. Extracellular vesicle protein concentration and the number of exosome vesicles were not affected by risk category; however, the exosome protein cargo differed between the groups, with proteins at each time point identified as being unique to the high- and low-risk groups. The proteins α-2 macroglobulin, fibrinogen, and oncoprotein-induced transcript 3 were unique to the high-risk cows on d 0 and have been associated with metabolic syndrome and liver function in humans. Their presence may indicate a more severe inflammatory state and a greater degree of liver dysfunction in the high-risk cows than in the low-risk cows, consistent with the high-risk cows' greater plasma β-hydroxybutyrate and liver triacylglycerol concentrations. The commonly shared proteins and those unique to the low-risk category indicate a role for exosomes in immune function. The data

  13. Muscular and Systemic Correlates of Resistance Training-Induced Muscle Hypertrophy (United States)

    Mitchell, Cameron J.; Churchward-Venne, Tyler A.; Bellamy, Leeann; Parise, Gianni; Baker, Steven K.; Phillips, Stuart M.


    Purpose To determine relationships between post-exercise changes in systemic [testosterone, growth hormone (GH), insulin like grow factor 1 (IGF-1) and interleukin 6 (IL-6)], or intramuscular [skeletal muscle androgen receptor (AR) protein content and p70S6K phosphorylation status] factors in a moderately-sized cohort of young men exhibiting divergent resistance training-mediated muscle hypertrophy. Methods Twenty three adult males completed 4 sessions•wk-1 of resistance training for 16 wk. Muscle biopsies were obtained before and after the training period and acutely 1 and 5 h after the first training session. Serum hormones and cytokines were measured immediately, 15, 30 and 60 minutes following the first and last training sessions of the study. Results Mean fiber area increased by 20% (range: -7 to 80%; Pmuscle fiber hypertrophy; however, the magnitude of the post exercise IL-6 response was correlated with muscle hypertrophy (r=0.48, P=0.019). Conclusion Post-exercise increases in circulating hormones are not related to hypertrophy following training. Exercise-induced changes in IL-6 correlated with hypertrophy, but the mechanism for the role of IL-6 in hypertrophy is not known. Acute increases, in p70S6K phosphorylation and changes in muscle AR protein content correlated with muscle hypertrophy implicating intramuscular rather than systemic processes in mediating hypertrophy. PMID:24130904

  14. Luteinizing hormone-releasing hormone fusion protein vaccines block estrous cycle activity in beef heifers. (United States)

    Stevens, J D; Sosa, J M; deAvila, D M; Oatley, J M; Bertrand, K P; Gaskins, C T; Reeves, J J


    Two LHRH fusion proteins, thioredoxin and ovalbumin, each containing seven LHRH inserts were tested for their ability to inhibit estrous cycle activity. The objective was to evaluate immune and biological responses from alternating the two fusion proteins in an immunization schedule. One hundred ten heifers were divided equally into 11 groups. Two control groups consisted of either spayed or intact, untreated heifers. Heifers in the other nine groups were immunized on wk 0, 4, and 9. Treatments were immunizations of the same protein throughout or alternating the proteins in different booster sequences. Blood was collected weekly for 22 wk, and serum was assayed for concentrations of progesterone and titers of anti-LHRH. At slaughter, reproductive tracts were removed from each heifer and weighed. Heifers with >or=1 ng/mL of progesterone were considered to have a functional corpus luteum and thus to have estrous cycle activity. All LHRH-immunized groups of heifers had a smaller (P spayed heifers during wk 9 to 22. Anti-LHRH did not differ among immunized groups during wk 1 to 9. Starting at wk 10 and continuing through the conclusion of the study, there was an overall difference among treatment groups for anti-LHRH (P spaying in suppression of estrous cycle activity, but alternating the two proteins in an immunization schedule did not enhance the immunological or biological effectiveness of the vaccine. PMID:15583055

  15. 高度近视白内障患者小切口非超声乳化术后角膜屈光力的变化%Analysis of corneal refractive power after small incision surgery of non -phacoemmulsification for high myopic eyes with cataract

    Institute of Scientific and Technical Information of China (English)

    叶霞; 宋平; 郭长梅


    目的::探讨中老年高度近视白内障患者小切口非超声乳化术后角膜屈光力的变化及手术源性角膜散光( SIA )的特点。方法:回顾性分析我院中老年高度近视白内障行小切口非超声乳化患者103例145眼,角膜曲率计测量术前及术后3d,1wk,1、3mo的角膜水平屈光力值(K1)、垂直屈光力值( K2)及其轴位,采用正弦定律和余弦定律计算法计算SIA值。结果:术后3d,1wk平均角膜散光度与术前相比,差别有统计学意义( P<0.05)。术后1 wk,1、3 mo SIA 值分别为+1.34±0.12,+0.89±0.27和-0.42±0.26,差别有统计学意义(P<0.05)。与术前相比较,术后3d, K1增大1.59D, K2减少1.98D,两者之间有统计学差异( P<0.01);术后3mo内K1和K2与术前的差值均迅速减小,术后3 mo内K1差值比K2差值绝对值小,两者之间各时间点差值的绝对值有统计学差异(P<0.05)。结论:中老年高度近视白内障患者小切口非超声乳化术后短期角膜散光较大,手术方式需进一步改进以减少散光。%AlM: To evaluate the changes of corneal refractive power and surgically induced astigmatism ( SlA ) after small incision non - phacoemmulsification surgery for high myopic eyes with cataract in middle aged and senile people.METHODS:A retrospective study was done in 145 eyes of 103 middle aged and senile patients with high myopia and cataract who underwent small incision non -phacoemulsification surgery in our hospital. The keratometer was used to measure corneal horizontal refractive power ( K1 ) , vertical corneal refractive power (K2) and their axial on postoperative 3d, 1wk, 1 and 3mo. SlA was calculated by sine law and cosine law method.RESULTS: There was statistically significant difference on average corneal astigmatism at 3d and 1wk compared with that before surgeries ( P < 0. 05 ). SlA were respectively +1.34±0.12, +0.89±0.27 and -0.42±0.26 at 1wk, 1 and 3mo postoperatively, and the differences were statistically

  16. Exercise volume and aerobic fitness in young adults: the Midwest Exercise Trial-2. (United States)

    Schubert, Matthew M; Washburn, Richard A; Honas, Jeffery J; Lee, Jaehoon; Donnelly, Joseph E


    To examine the effect of exercise volume at a fixed intensity on changes in aerobic fitness. Ninety-two overweight/obese individuals (BMI 25-40 kg m(2)), age 18-30 years, 50 % women, completed a 10 mo, 5 d wk(-1) supervised exercise intervention at 2 levels of exercise energy expenditure (400 or 600 kcal session(-1)) at 70-80 % heart rate (HR) max. Exercise consisted primarily of walking/jogging on motor-driven treadmills. The duration and intensity of all exercise sessions were verified by a downloadable HR monitor set to collect HR in 1-min epochs. All participants were instructed to continue their typical patterns of non-exercise physical activity and dietary intake over the duration of the 10 mo intervention. Maximal aerobic capacity (indirect calorimetry) was assessed on a motor-driven treadmill using a modified Balke protocol at baseline, mid-point (5 mo), and following completion of the 10 mo intervention. VO2 max (L min(-1)) increased significantly in both the 400 (11.3 %) and 600 kcal session(-1) groups (14 %) compared to control (-2.0 %; p young adults. PMID:27026879

  17. Outcome comparison between transcanalicular and external dacryocystorhinostomy

    Institute of Scientific and Technical Information of China (English)

    Gunay; Uludag; Baris; Yeniad; Erdinc; Ceylan; Ayse; Yildiz-Tas; Lale; Kozer-Bilgin


    AIM: To compare the outcomes achieved with external dacryocystorhinostomy(EX-DCR) and transcanalicular dacryocystorhinostomy(TC-DCR) using a multidiode laser in patients with bilateral nasolacrimal duct obstruction(NLDO).METHODS: This prospective study was conducted on38 eyes of 19 patients with bilateral NLDO. Simultaneous bilateral surgery was performed on all patients. TC-DCR(Group 1) with a diode laser was used in the right eye,and EX-DCR(Group 2) was used in the left eye. All patients were placed under general anesthesia. Routine follow-ups were scheduled at 1wk; 1, 3, 6 and 12 mo postoperative intervals. Objective(lacrimal system irrigation) and subjective [tearing, irritation, pain,discharge and visual analogue scale(VAS) score]outcomes were evaluated.RESULTS: The overall objective success rate at 12 mo was 73.7%(14/19) in Group 1 and 89.5 %(17/19) in Group2. This difference was statistically significant. There were no significant between-group differences in the subjective results, such as tearing, pain and irritation.Only the discharge scores were found to be significantly higher in Group 1 compared to Group 2 at the 1y followup. The average VAS score was 6.8 in Group 1 and 8.7 in Group 2, with no statistically significant differences.CONCLUSION: Although TC-DCR allows surgeons to perform a minimally invasive and safe procedure, EX-DCR offers better objective and subjective outcomes than TC-DCR.

  18. Influence of recreational activity and muscle strength on ulnar bending stiffness in men (United States)

    Myburgh, K. H.; Charette, S.; Zhou, L.; Steele, C. R.; Arnaud, S.; Marcus, R.


    Bone bending stiffness (modulus of elasticity [E] x moment of inertia [I]), a measure of bone strength, is related to its mineral content (BMC) and geometry and may be influenced by exercise. We evaluated the relationship of habitual recreational exercise and muscle strength to ulnar EI, width, and BMC in 51 healthy men, 28-61 yr of age. BMC and width were measured by single photon absorptiometry and EI by mechanical resistance tissue analysis. Maximum biceps strength was determined dynamically (1-RM) and grip strength isometrically. Subjects were classified as sedentary (S) (N = 13), moderately (M) (N = 18), or highly active (H) (N = 20) and exercised 0.2 +/- 0.2; 2.2 +/- 1.3; and 6.8 +/- 2.3 h.wk-1 (P ulnar BMC (P ulnar EI (P = 0.01) than M or S, who were similar. Amount of activity correlated with grip and biceps strength (r = 0.47 and 0.49; P ulnar width (P Ulnar width and biceps strength were the only independent predictors of EI (r2 = 0.67, P ulnar bending stiffness.


    Directory of Open Access Journals (Sweden)

    Rodney A. Kennedy


    Full Text Available Despite its obvious advantages, few studies have examined health outcomes of regular stariclimbing. In this study, we investigated the training effects of eight weeks of stairclimbing on recognised measures of health-related fitness in an occupational setting. Forty-five public sector employees (22 male, 23 female aged 42.3 ± 9.0 years were randomly assigned to control (n = 16 or stairclimbing (n = 29 groups. Stairclimbing training began with 1 bout 5d·wk-1 in week 1, increasing by one climb per day every two weeks until week 5, where a maintenance level of 3 climbs per day was reached. Participants climbed on staircases located within an 8 storey office block, consisting of 145 steps. The prescribed exercise intensity involved climbing the 8 flights of stairs at a rate of 75 steps·min-1. All participants agreed not to change their diet or lifestyle over the experimental period. Relative to controls, the stairclimbing group showed a significant increase of 9.4% in predicted VO2max (p < 0. 05. No significant changes in blood pressure, blood lipid concentrations or body composition were noted. These findings provide evidence that stairclimbing can enhance an important component of health-related fitness, namely cardiovascular fitness. Given that such improvement resulted from less than 30 minutes per week of moderate exercise, stairclimbing in the workplace should be promoted as a health-enhancing physical activity


    Directory of Open Access Journals (Sweden)

    Miron Kovačić


    Full Text Available Thermal conductivity (KTV of geological formations is one of the parameters responsible for the propagation of the heat under the earth surface. During geothermal investigations in the broader area of the Croatian capital of Zagreb the thermal conductivity was measured on the rock samples from the surface and the boreholes. The results of the measurements are presented in this work and used as a basis for calculations of the thermal conductivity of distinct geological formations within the investigated area. It was found out that the values of the thermal conductivity of the rocks in the investigated area vary greatly. The measurements are within the well known scope for certain rock types. The thermal conductivity of the rocks from the Tertiary units corresponds with the average values being typical for such kind of rocks, while the basement carbonate rocks are characterized by the values being by 1 W/K-1m-1 higher than the average. After comparing the thermal conductivity of the stratigraphic units in the broader area of Zagreb it has been established that the values of the thermal conductivity of geological formations in the investigated area are also very different, and that they generally rise with their age. The relative relationships show that the Quaternary, Pliocene and Tertiary sedimentary rocks act as thermal insulators, while Triassic rocks behave as the heat conductor (the paper is published in Croatian.

  1. KARAKTERISTIK FISIK, KIMIA, MIKROBIOLOGI WHEY KEFIR DAN AKTIVITASNYA TERHADAP PENGHAMBATAN ANGIOTENSIN CONVERTING ENZYME (ACE [Physical, Chemical and Microbiological Characteristics of Whey Kefir and Its Angiotensin Converting Enzyme (ACE Inhibitory Activity

    Directory of Open Access Journals (Sweden)

    Andi Febrisiantosa*


    Full Text Available This study was conducted to evaluate the characteristics of whey-based kefir products and their activity to inhibit the angiotensin converting enzyme (ACE. Kefir was produced by using many types of whey, namely SK: skim milk based kefir (control; WK: gouda cheese whey based kefir; and WKB: commercial whey powder based kefir. The experimental design was a completely randomized design. Each treatment was conducted in triplicates. Kefirs were evaluated for physical and chemical properties (pH, total titratable acidity, viscosity, protein, fat, lactose, and alcohol, microbiological (lactic acid bacteria and yeast population, peptide concentration, ACE inhibition, IC50 and Inhibition Efficiency Ratio (IER. The results showed that the types of whey used for kefir productions significantly affected the physical and chemical characteristics of the products (p0.05. The peptide concentration and ACE inhibitory activity of WK, 1.54±0.02 mg/mL and 73.07±0.91%, was significantly higher (p0.05 from the control (47.19±0.09% per mg/mL but was significantly higher (p<0.05 than that of WKB (45.75±0.18% per mg/mL. This research indicated that whey kefir is a potential source of bioactive peptide for antihypertention agent.

  2. Rubber band ligation for 750 cases of symptomatic hemorrhoids out of 2200 cases

    Institute of Scientific and Technical Information of China (English)

    Ayman M El Nakeeb; Hisham H Abd Elmoniam; Waiel W Khafagy; Mosaad H Horshed; Ramadan E El Lithy; Mohamed E Farid; Amir A Fikry; Waleed H Omar; Elyamani M Fouda; Tito A El Metwally; Hosam E Ghazy; Sabry A Badr; Mohmed Y Abu Elkhar; Salih M Elawady


    AIM: To study the results for the treatment of symptomatic hemorrhoids using rubber band ligation (RBL) method. METHODS: A retrospective study for 750 patients who came to the colorectal unit from June, 1998 to September, 2006, data was retrieved from archived files. RBL was performed using the Mc Gown applicator on an outpatient basis. The patients were asked to return to out-patient clinic for follow up at 2 wk, 1 mo, 6 mo and through telephone call every 6 mo for 2 years). RESULTS: After RBL, 696 patients (92.8%) were cured with no difference in outcome for second or third degree hemorrhoids (P = 0.31). Symptomatic recurrence was detected in 11.04% after 2 years. A total of 52 patients (6.93%) had 77 complications from RBL which required no hospitalization. Complications were pain, rectal bleeding and vaso-vagal symptoms (4.13%, 4.13% and 1.33% of patients, respectively). At 1 mo there were a significant improvement in mean SF-36 scores over baseline in five items, while after 2 years there were improvement in all items over baseline, but not significant. No significant manometeric changes after band ligation. CONCLUSION: RBL is a simple, safe and effective method for treating symptomatic second and third degree hemorrhoids as an out patient procedure with significant improvement in quality of life. RBL doesn't alter ano-rectal functions.

  3. The effects of an ActRIIb receptor Fc fusion protein ligand trap in juvenile simian immunodeficiency virus-infected rhesus macaques. (United States)

    O'Connell, Karyn E; Guo, Wen; Serra, Carlo; Beck, Matthew; Wachtman, Lynn; Hoggatt, Amber; Xia, Dongling; Pearson, Chris; Knight, Heather; O'Connell, Micheal; Miller, Andrew D; Westmoreland, Susan V; Bhasin, Shalender


    There are no approved therapies for muscle wasting in children infected with human immunodeficiency virus (HIV), which portends poor disease outcomes. To determine whether a soluble ActRIIb receptor Fc fusion protein (ActRIIB.Fc), a ligand trap for TGF-β/activin family members including myostatin, can prevent or restore loss of lean body mass and body weight in simian immunodeficiency virus (SIV)-infected juvenile rhesus macaques (Macaca mulatta). Fourteen pair-housed, juvenile male rhesus macaques were inoculated with SIVmac239 and, 4 wk postinoculation (WPI) treated with intramuscular injections of 10 mg ⋅ kg(-1) ⋅ wk(-1) ActRIIB.Fc or saline placebo. Body weight, lean body mass, SIV titers, and somatometric measurements were assessed monthly for 16 wk. Age-matched SIV-infected rhesus macaques were injected with saline. Intervention groups did not differ at baseline. Gains in lean mass were significantly greater in the ActRIIB.Fc group than in the placebo group (P fat mass in juvenile SIV-infected rhesus macaques. PMID:25466897

  4. A genome scan for quantitative trait loci affecting the Salmonella carrier-state in the chicken

    Directory of Open Access Journals (Sweden)

    Bumstead Nat


    Full Text Available Abstract Selection for increased resistance to Salmonella colonisation and excretion could reduce the risk of foodborne Salmonella infection. In order to identify potential loci affecting resistance, differences in resistance were identified between the N and 61 inbred lines and two QTL research performed. In an F2 cross, the animals were inoculated at one week of age with Salmonella enteritidis and cloacal swabs were carried out 4 and 5 wk post inoculation (thereafter called CSW4F2 and CSW4F2 and caecal contamination (CAECF2 was assessed 1 week later. The animals from the (N × 61 × N backcross were inoculated at six weeks of age with Salmonella typhimurium and cloacal swabs were studied from wk 1 to 4 (thereafter called CSW1BC to CSW4BC. A total of 33 F2 and 46 backcross progeny were selectively genotyped for 103 and 135 microsatellite markers respectively. The analysis used least-squares-based and non-parametric interval mapping. Two genome-wise significant QTL were observed on Chromosome 1 for CSW2BC and on Chromosome 2 for CSW4F2, and four suggestive QTL for CSW5F2 on Chromosome 2, for CSW5F2 and CSW2BC on chromosome 5 and for CAECF2 on chromosome 16. These results suggest new regions of interest and the putative role of SAL1.

  5. Anomia treatment platform as behavioral engine for use in research on physiological adjuvants to neurorehabilitation

    Directory of Open Access Journals (Sweden)

    Diane Kendall, PhD


    Full Text Available The purpose of this study was to create a "behavioral treatment engine" for future use in research on physiological adjuvants in aphasia rehabilitation. We chose the behavioral target anomia, which is a feature displayed by many persons who have aphasia. Further, we wished to saturate the treatment approach with many strategies and cues that have been empirically reported to have a positive influence on aphasia outcome, with the goal being to optimize the potential for positive response in most participants. A single-subject multiple baseline design with replication across eight participants was employed. Four men and four women, with an average age of 62 yr and an average of 63.13 mo poststroke onset, served as participants. Word-retrieval treatment was administered 3 d/wk, 1 h/d for a total of 20 treatment hours (6–7 wk. Positive acquisition effects were evident in all eight participants (d effect size [ES] = 5.40. Treatment effects were maintained 3 mo after treatment termination for five participants (d ES = 2.94. Within and across semantic category, generalization was minimal (d ES = 0.43 within and 1.09 across. This study demonstrates that this behavioral treatment engine provides a solid platform on which to base future studies whereby various treatment conditions are manipulated and pharmacologic support is added.

  6. The effect of a worksite based walking programme on cardiovascular risk in previously sedentary civil servants [NCT00284479

    Directory of Open Access Journals (Sweden)

    Hare Lesley G


    Full Text Available Abstract Background A significant proportion of Europeans do not meet the recommendations for 30 mins of physical activity 5 times per week. Whether lower frequency, moderate intensity exercise alters cardiovascular disease (CVD risk has received little attention. This study examined the effects of 45 minutes self-paced walking, 2 d· wk-1 on aerobic fitness, blood pressure (BP, body composition, lipids and C-Reactive Protein (CRP in previously sedentary civil servants. Methods 37 subjects (24 women aged 41.5 ± 9.3 years were randomly assigned to either two 45 minute walks per week (walking group or no training (control group. Aerobic fitness, body composition, blood pressure (BP, CRP and lipoprotein variables were measured at baseline and following 8 weeks. Steps counts were measured at baseline and during weeks 4 and 8 of the intervention. Results Compared to the control group, the walking group showed a significant reduction in systolic BP and maintained body fat levels (P P Conclusion These findings suggest that walking twice per week for 45 minutes at ~ 62% HRmax, improves activity levels, reduces systolic BP and prevents an increase in body fat in previously sedentary adults. This walking prescription, however, failed to induce significant improvements in other markers of cardiovascular disease risk following eight weeks of training.

  7. Comparison of escitalopram and paroxetine in treatment of panic disorder%艾司西酞普兰与帕罗西汀治疗惊恐障碍对照观察

    Institute of Scientific and Technical Information of China (English)

    蓝利明; 朱培俊; 方伟均; 蔡进伟; 吴素琴; 王静


    目的 对照观察艾司西酞普兰与帕罗西汀对惊恐障碍患者的疗效及安全性.方法 将68例惊 恐障碍患者随机分为艾司西酞普兰组(n=36)和帕罗西汀组(n=32).艾司西酞普兰wkl剂量为10 mg·d-1,wk 2剂量为15 mg·d-1,wk 3剂量加至15~20 mg·d-1;帕罗西汀wk 1剂量为20 mg·d-1,wk 2为30 mg·d-1,wk 4酌情加量至40 mg·d-1 (bid).疗程8 wk.用惊恐症状评定量表(PASS)、汉密尔顿焦虑量表(HAMA)、汉密尔顿抑郁量表17项(HAMD-17)、副反应量表(TESS)对患者治疗前后进行评估.结果 艾司西酞普兰组有33例、帕罗西汀组有31例完成了治疗.与基线相比,艾司西酞普兰组HAMD和HAMA总分分别从wk 2和wk 1末开始有明显下降(P<0.05),帕罗西汀组则分别在wk 4和wk 2末开始下降(P<0.05);治疗终点时2组总分变化均较基线有非常显著差异(P<0.01),2组PASS减分值分别为(9.6±3.8)和(9.1±3.4),较基线明显改善(均P< 0.01),而2组之间无显著差异(P>0.05).艾司西酞普兰组治愈率、有效率分别为85%、91%,帕罗西汀组分别为81%、90%,2组无显著差异(P>0.05).2组不良反应发生率无显著差异(P> 0.05).结论 艾司西酞普兰治疗惊恐障碍的疗效、安全性与帕罗西汀相当,而起效较快.%AIM To investigate the clinical efficacy and safety of escitalopram in treating patients with panic disorder. METHODS Sixty-eight patients with panic disorder were randomized divided into escitalopram group (n = 36) and paroxetine group (re = 32). The dosage of escitalopram was 10 mg·d-1 (wk 1), 15 mg·d-1 (wk 2) and 15 - 20 mg·d-1 (wk 3) and the dosage of paroxetine was 20 mg·d-1 (wk 1), 30 mg·d-1 (wk 2) and 40 mg·d-1 (bid, wk 4). The course of the treatment was eight weeks for each group. All the patients were rated by PASS, HAMA, HAMD-17 and TESS before and after the treatment. RESULTS Thirty-three patients of the escitalopram group and 31 patients of the paroxetine group had

  8. Hydrochemical data base for the Death Valley Region, California and Nevada

    International Nuclear Information System (INIS)

    Ground-water chemistry data derived from samples collected within an approximately 100,000-square-kilometer area in the Southern Great Basin have been compiled into a digital data base. The data were compiled from published reports, the U.S. Geological Survey (USGS) National Water Information System (NWIS), and previously unpublished USGS files. The data are contained in two compressed files which self-expand into Lotus (.WK1) files. The first file contains 4,738 records (4.84 megabytes) and represents the basic compilation of all identified analyses. The second file is an edited version of the first and contains 3,733 records (3.84 megabytes). Editing included the removal of duplicate records and the combining of records, when appropriate. The analyses presented are of variable quality and comprehensiveness and include no isotopic data. Of the 3,733 analyses in the edited data base, 58 percent of the major ion concentrations balance to within ±10 percent. Most of the remaining records are not sufficiently complete for a balance to be calculated

  9. Effect of the chosen incision on corneal astigmatism after implantable collamer lens surgery

    Directory of Open Access Journals (Sweden)

    Xia Jiang


    Full Text Available AIM: To evaluate the effect of the chosen incision on corneal astigmatism after implantable collamer lens(ICLsurgery.METHODS: The study included 195 eyes of 102 patients, and all eyes were randomly divided into two groups: the chosen incision group(Group A, 97 eyesand temporal corneal incision(Group B, 98 eyes. Before the operation, and 1wk,1mo and 3mo after the operation, each patient was examined with corneal topography to observe the changes of corneal astigmatism. RESULTS: Preoperative corneal astigmatisms were(1.26±0.35D in group A and(1.28±0.38D in group B, thus there was no statistically significant difference(P>0.05. One week postoperatively, the astigmatism were(0.93±0.29D in group A and(1.32±0.33D in group B. One month postoperatively, the astigmatism were(0.85±0.16D in group A and(1.27±0.18D in group B. Three months postoperatively, the astigmatism were(0.80±0.13D in group A and(1.25±0.20D in group B. The differences between the two groups were statistically significant(PCONCLUSION: The chosen incision can reduce postoperative astigmatism to a certain extent after ICL surgery.

  10. Steroids reduce local inflammatory mediator secretion and mucosal permeability in collagenous colitis patients

    Institute of Scientific and Technical Information of China (English)

    Yesuf Taha; Yngve Raab; Marie Carlson; Anders Larsson; Mikael L(o)rdal; Lars L(oo)f; Magnus Th(o)rn


    AIM: To study the effect of oral steroids upon clinical response and rectal mucosa secretion of eosinophil cationic protein (ECP), myeloperoxidase (MPO), basic fibroblast growth factor (bFGF), vascular endothelial growth factor (VEGF) and albumin in patients with collagenous colitis (CC).METHODS: A segmental perfusion technique was used to collect perfusates from rectum of CC patients once before and twice (one and four weeks) after the start of steroid treatment. Clinical data was monitored and ECP, MPO, bFGF, VEGF and albumin concentrations were analyzed by immunochemical methods in perfusates and in serum.RESULTS: Steroids reduced the number of bowel movements by more than five times within one week and all patients reported improved subjective wellbeing at wk 1 and 4. At the same time, the median concentrations of ECP, bFGF, VEGF and albumin in rectal perfusates decreased significantly. MPO values were above the detection limit in only 3 patients before treatment and in none during treatment. VEGF, bFGF,ECP and albumin concentrations correlated with each other with the exception of ECP and albumin. A decrease of serum ECP and VEGF concentrations was also seen even if the overtime reduction was not significant.CONCLUSION: Oral steroid treatment in CC patients induced a simultaneous reduction of bowel movements and rectal release of ECP, bFGF, VEGF and albumin,suggesting that these polypeptides and increased mucosal permeability are important components of the pathophysiology in collagenous colitis.

  11. Transference of kettlebell training to strength, power, and endurance. (United States)

    Manocchia, Pasquale; Spierer, David K; Lufkin, Adrienne K S; Minichiello, Jacqueline; Castro, Jessica


    Kettlebells are a popular implement in many strength and conditioning programs, and their benefits are touted in popular literature, books, and videos. However, clinical data on their efficacy are limited. The purpose of this study was to examine whether kettlebell training transfers strength and power to weightlifting and powerlifting exercises and improves muscular endurance. Thirty-seven subjects were assigned to an experimental (EXP, n = 23; mean age = 40.9 ± 12.9 years) or a control group (CON; n = 14; mean age = 39.6 ± 15.8 years), range 18-72 years. The participants were required to perform assessments including a barbell clean and jerk, barbell bench press, maximal vertical jump, and 45° back extensions to volitional fatigue before and after a 10-week kettlebell training program. Training was structured in a group setting for 2 d·wk(-1) for 10 weeks. A repeated measures analysis of variance was conducted to determine group × time interactions and main effects. Post hoc pairwise comparisons were conducted when appropriate. Bench press revealed a time × group interaction and a main effect (p kettlebells. Traditional training methods may not be convenient or accessible for strength and conditioning specialists, athletes, coaches, and recreational exercisers. The current data suggest that kettlebells may be an effective alternative tool to improve performance in weightlifting and powerlifting. PMID:22549084

  12. The relation between anthropometric and physiological variables and bat velocity of high-school baseball players before and after 12 weeks of training. (United States)

    Szymanski, David J; Szymanski, Jessica M; Schade, Ryan L; Bradford, T Jason; McIntyre, Joseph S; DeRenne, Coop; Madsen, Nels H


    The purpose of this article was to investigate the relation between anthropometric and physiological variables to linear bat swing velocity (BV) of 2 groups of high-school baseball players before and after completing a 12-week periodized resistance exercise program. Participants were randomly assigned to 1 of 2 training groups using a stratified sampling technique. Group 1 (n = 24) and group 2 (n = 25) both performed a stepwise periodized resistance exercise program and took 100 swings a day, 3 d·wk-1, for 12 weeks with their normal game bat. Group 2 performed additional rotational and full-body medicine ball exercises 3 d·wk-1 for 12 weeks. Fourteen variables were measured or calculated before and after 12 weeks of training. Anthropometric and physiological variables tested were height, body mass, percent body fat, lean body mass (LBM), dominant torso rotational strength (DTRS) and nondominant torso rotational strength (NDTRS), sequential hip-torso-arm rotational strength measured by a medicine ball hitter's throw (MBHT), estimated 1 repetition maximum parallel squat (PS) and bench press (BP), vertical jump (VJ), estimated peak power, angular hip velocity (AHV), and angular shoulder velocity (ASV). The baseball-specific skill of linear BV was also measured. Statistical analysis indicated a significant moderately high positive relationship (p ≤ 0.05) between prelinear BV and pre-NDTRS for group 1, pre-LBM, DTRS, NDTRS, peak power, and ASV for group 2; moderate positive relationship between prelinear BV and preheight, LBM, DTRS, peak power, BP, PS, and ASV for group 1, preheight, body mass, MBHT, BP, and PS for group 2. Significantly high positive relationships were indicated between postlinear BV and post-NDTRS for group 1, post-DTRS and NDTRS for group 2; moderately high positive relationships between postlinear BV and post-LBM, DTRS, peak power, BP, and PS for group 1, postheight, LBM, VJ, peak power for group 2; moderate positive relationships between

  13. Haemoglobin mass and running time trial performance after recombinant human erythropoietin administration in trained men.

    Directory of Open Access Journals (Sweden)

    Jérôme Durussel

    Full Text Available UNLABELLED: Recombinant human erythropoietin (rHuEpo increases haemoglobin mass (Hb(mass and maximal oxygen uptake (v O(2 max. PURPOSE: This study defined the time course of changes in Hb(mass, v O(2 max as well as running time trial performance following 4 weeks of rHuEpo administration to determine whether the laboratory observations would translate into actual improvements in running performance in the field. METHODS: 19 trained men received rHuEpo injections of 50 IU•kg(-1 body mass every two days for 4 weeks. Hb(mass was determined weekly using the optimized carbon monoxide rebreathing method until 4 weeks after administration. v O(2 max and 3,000 m time trial performance were measured pre, post administration and at the end of the study. RESULTS: Relative to baseline, running performance significantly improved by ∼6% after administration (10:30±1:07 min:sec vs. 11:08±1:15 min:sec, p<0.001 and remained significantly enhanced by ∼3% 4 weeks after administration (10:46±1:13 min:sec, p<0.001, while v O(2 max was also significantly increased post administration (60.7±5.8 mL•min(-1•kg(-1 vs. 56.0±6.2 mL•min(-1•kg(-1, p<0.001 and remained significantly increased 4 weeks after rHuEpo (58.0±5.6 mL•min(-1•kg(-1, p = 0.021. Hb(mass was significantly increased at the end of administration compared to baseline (15.2±1.5 g•kg(-1 vs. 12.7±1.2 g•kg(-1, p<0.001. The rate of decrease in Hb(mass toward baseline values post rHuEpo was similar to that of the increase during administration (-0.53 g•kg(-1•wk(-1, 95% confidence interval (CI (-0.68, -0.38 vs. 0.54 g•kg(-1•wk(-1, CI (0.46, 0.63 but Hb(mass was still significantly elevated 4 weeks after administration compared to baseline (13.7±1.1 g•kg(-1, p<0.001. CONCLUSION: Running performance was improved following 4 weeks of rHuEpo and remained elevated 4 weeks after administration compared to baseline. These field performance effects coincided with r

  14. Associations of peripartum markers of stress and inflammation with milk yield and reproductive performance in Holstein dairy cows. (United States)

    Huzzey, Juliana M; Mann, Sabine; Nydam, Daryl V; Grant, Richard J; Overton, Thomas R


    The objective was to evaluate the association of peripartum concentrations of fecal cortisol metabolites (11,17-dioxoandrostane; 11,17-DOA), plasma cortisol and haptoglobin (Hp), as well as two markers of negative energy balance, non-esterified fatty acid (NEFA) and postpartum β-hydroxybutyrate (BHBA), with milk yield and reproductive performance. Blood and fecal samples were collected weekly from 412 Holstein dairy cows from wk -3 through wk +1 relative to calving. Pregnancies by 150 days in milk (DIM) and projected 305-d mature equivalent (305ME) milk yield based on the 3rd Dairy Herd Improvement (DHI) test day (mean±SD; 102±17 DIM) were measured. Multivariable linear regression models were used to describe the associations of metabolites with 305ME milk yield. Semiparametric proportional hazards models were used to describe associations of the same metabolites with risk of conception by 150 DIM. Negative associations with milk yield were found for prepartum Hp in wk -2, -1, and +1 relative to calving (estimate±SE: 490±251, 564±259, and 464±136kg lower yield for every increase in Hp concentration by 1g/L, respectively) as well as with NEFA concentration in wk -2 (estimate±SE: 1465±541kg lower milk yield for an increase in NEFA concentration by 1mEq/L). Postpartum associations of NEFA with milk yield depended on parity; NEFA was associated with an increase in milk yield in primiparous animals only (estimate±SE: 1548±510kg increase for an increase in NEFA concentration by 1mEq/L). An increase in plasma cortisol concentration by 1μg/dL in wk +1 relative to calving was associated with an increase in milk yield (estimate±SE: 580±176kg). Prepartum 11,17-DOA was associated in all three prepartum sampling weeks with a reduced hazard ratio (HR) of conception (HR [95% CI]: 0.81 [0.67-0.97], 0.85 [0.72-0.99], and 0.85 [0.75-0.97] for every increase in concentration by 1mg/g fecal dry matter (DM) in wk -3, -2, and -1 relative to calving, respectively

  15. Effect of prepartum administration of recombinant bovine somatotropin on health and performance of lactating dairy cows. (United States)

    Gohary, K; LeBlanc, S J; Lissemore, K D; Overton, M W; Von Massow, M; Duffield, T F


    A double-blind, randomized clinical trial was conducted in 5 commercial dairy herds in southern Ontario with 1,362 cows enrolled to evaluate the effect of prepartum administration of recombinant bovine somatotropin (rbST) on health and performance. Cows were randomly assigned to receive either 325 mg of sometribove zinc suspension (n=680) or a placebo injection (n=682; control) subcutaneously every 14 d until calving. Treatments started 28 to 22 d before expected calving, with a maximum of 3 treatments per cow. Serum samples taken at the time of enrollment, 1 wk before calving, and weekly for 3 wk after calving were analyzed for nonesterified fatty acids (NEFA), β-hydroxybutyrate (BHBA), glucose, aspartate aminotransferase, calcium, and haptoglobin. Diseases were recorded by farm staff. Incidences of clinical ketosis, clinical mastitis, displaced abomasum, metritis, retained placenta, milk fever, and lameness were similar between groups. Body condition score was lower for treated than for control cows at 3 wk after calving (3.13 and 3.17, respectively). Serum NEFA tended to be higher for treated than for control cows by 0.01 mmol/L. Overall BHBA was not different between groups, but BHBA for treated cows was higher in wk 1 after calving (750 and 698 μmol/L, respectively) and tended to be higher in wk 2 after calving (779 and 735 μmol/L, respectively). Incidence of hyperketonemia was similar between groups. Treated cows had higher serum glucose compared with control cows (2.8 and 2.7 mmol/L, respectively). We detected no differences in serum aspartate aminotransferase, calcium, or haptoglobin between groups. Milk yield was recorded daily for each cow for 63 d, and did not differ between groups (37.1 ± 0.5 kg and 36.7 ± 0.5 kg, respectively) but we detected a tendency for treated cows to produce 0.8 kg/d more milk than control cows in wk 1 after calving. We observed no difference between groups in the time from calving to first insemination or the probability of


    Ramsay, Edward; Sadler, Ryan; Rush, Robert; Seimon, Tracie; Tomaszewicz, Ania; Fleetwood, Ellen A; McAloose, Denise; Wilkes, Rebecca P


    Three methods for delivering a live attenuated canine distemper virus (CDV) vaccine to domestic cats ( Felis catus ) were investigated, as models for developing vaccination protocols for tigers (Panthera tigris). Twenty domestic cats were randomly divided into four treatment groups: saline injection (negative controls); and oral, intranasal, and subcutaneous vaccinates. Cats were injected with saline or a CDV vaccine (Nobivac DP, Merck) at wk 0 and 4. Blood and nasal swabs were collected at wk 0 (prior to the initial vaccination) and weekly thereafter for 9 wk. Urine samples were collected on wk 1 to 9 after initial vaccination. Forty-nine weeks following the initial vaccination series, three cats from the subcutaneous group and three cats from the intranasal group were revaccinated. Blood was collected immediately prior, and 7 and 21 days subsequent to revaccination. Nasal swabs and urine samples were collected from each cat prior to wk 49 revaccination and daily for 7 days thereafter. Nasal swabs and urine were analyzed by quantitative PCR for vaccine virus presence. Sera were tested for CDV antibodies by virus neutralization. All cats were sero-negative for CDV antibodies at the beginning of the study, and saline-injected cats remained sero-negative throughout the study. A dramatic anamnestic response was seen following wk 4 subcutaneous vaccinations, with titers peaking at wk 6 (geometric mean = 2,435.5). Following wk 49 revaccination, subcutaneous vaccinates again mounted impressive titers (wk 52 geometric mean = 2,048). Revaccination of the intranasal group cats at wk 49 produced a small increase in titers (wk 52 geometric mean = 203). CDV viral RNA was detected in six nasal swabs but no urine samples, demonstrating low viral shedding postvaccination. The strong antibody response to subcutaneous vaccination and the lack of adverse effects suggest this vaccine is safe and potentially protective against CDV infection in domestic cats. PMID:27468028


    Directory of Open Access Journals (Sweden)



    Full Text Available Extrauterine abdominal pregnancy is extremely rare and is frequently missed during antenatal care, despite the routine use of obstetrics ultrasound. A 23 year old primigravida at 39 weeks of gestation was admitted to the department on December 2 , 2012, in routine hour with transverse lie with oligohydramnios. On examination her vitals and laboratory investigation were within normal limits . One of the ultrasonographic scanning done by radiologist at 35 wk. 3 days gestation demonstrated a single live intrauterine fetus of CGA 33 wk. 1 day with transverse lie , fundal gr II placenta and AFI - 10 cm. Ultrasonography done at the department on the day of admission demonstrated single live intrauterine fetus with transverse lie and AFI - 3.8 cm and EFW 2.4 Kg and placenta was right anterior.. She was planned for elective caesarean section on account of transverse lie and oligohydramnios at term. At laparotomy the following findings were made: Secondary abdominal pregnancy arising from right tubal ectopic rupture and making pseudogesta tional sac with meconium stained liquor. Omentum , right tube and ovary were adhered to sac. The uterus and left tube and ovary were healthy and normal . Other abdominal organs were normal. A 1.5 Kg live male baby with good Apgar score and incompletely formed mandible extracted from pseudo gestational sac. There was significant bleeding from pseudogestational sac and patient went in shock during intraoperative period. One unit whole blood transfused during intraoperative period. Placenta was adherent to the pseudogestational sac so Psudogestational sac was removed with placenta in situ and right sided salpingo - o ophrectomy was also done due to their adherence. Hemostasis was secured. The patient and baby progressed well and were discharged on the seventh postoperative day

  18. Clinical ketosis and standing behavior in transition cows. (United States)

    Itle, A J; Huzzey, J M; Weary, D M; von Keyserlingk, M A G


    Ketosis is a common disease in dairy cattle, especially in the days after calving, and it is often undiagnosed. The objective of this study was to compare the standing behavior of dairy cows with and without ketosis during the days around calving to determine if changes in this behavior could be useful in the early identification of sick cows. Serum β-hydroxybutyrate (BHBA) was measured in 184 cows on a commercial dairy farm twice weekly from 2 to 21d after calving. Standing behavior was measured from 7d before calving to 21d after calving using data loggers. Retrospectively, 15 cows with clinical ketosis (3 consecutive BHBA samples >1.2mmol/L and at least one sample of BHBA >2.9mmol/L) were matched with 15 nonketotic cows (BHBA ketosis occurred 4.5±2.1d after calving. Total daily standing time was longer for clinically ketotic cows compared with nonketotic cows during wk -1 (14.3±0.6 vs. 12.0±0.7h/d) and on d 0 (17.2±0.9 vs. 12.7±0.9h/d) but did not differ during the other periods. Clinically ketotic cows exhibited fewer standing bouts compared with nonketotic cows on d 0 only (14.6±1.9 vs. 20.9±1.8bouts/d). Average standing bout duration was also longer for clinically ketotic cows on d 0 compared with nonketotic cows [71.3min/bout (CI: 59.3 to 85.5) vs. 35.8min/bout (CI: 29.8 to 42.9)] but was not different during the other periods. Differences in standing behavior in the week before and on the day of calving may be useful for the early detection of clinical ketosis in dairy cows. PMID:25465623

  19. Systemic metabolic derangement, pulmonary effects, and insulin insufficiency following subchronic ozone exposure in rats. (United States)

    Miller, Desinia B; Snow, Samantha J; Henriquez, Andres; Schladweiler, Mette C; Ledbetter, Allen D; Richards, Judy E; Andrews, Debora L; Kodavanti, Urmila P


    Acute ozone exposure induces a classical stress response with elevated circulating stress hormones along with changes in glucose, protein and lipid metabolism in rats, with similar alterations in ozone-exposed humans. These stress-mediated changes over time have been linked to insulin resistance. We hypothesized that acute ozone-induced stress response and metabolic impairment would persist during subchronic episodic exposure and induce peripheral insulin resistance. Male Wistar Kyoto rats were exposed to air or 0.25ppm or 1.00ppm ozone, 5h/day, 3 consecutive days/week (wk) for 13wks. Pulmonary, metabolic, insulin signaling and stress endpoints were determined immediately after 13wk or following a 1wk recovery period (13wk+1wk recovery). We show that episodic ozone exposure is associated with persistent pulmonary injury and inflammation, fasting hyperglycemia, glucose intolerance, as well as, elevated circulating adrenaline and cholesterol when measured at 13wk, however, these responses were largely reversible following a 1wk recovery. Moreover, the increases noted acutely after ozone exposure in non-esterified fatty acids and branched chain amino acid levels were not apparent following a subchronic exposure. Neither peripheral or tissue specific insulin resistance nor increased hepatic gluconeogenesis were present after subchronic ozone exposure. Instead, long-term ozone exposure lowered circulating insulin and severely impaired glucose-stimulated beta-cell insulin secretion. Thus, our findings in young-adult rats provide potential insights into epidemiological studies that show a positive association between ozone exposures and type 1 diabetes. Ozone-induced beta-cell dysfunction may secondarily contribute to other tissue-specific metabolic alterations following chronic exposure due to impaired regulation of glucose, lipid, and protein metabolism. PMID:27368153

  20. Uterine gland development begins postnatally and is accompanied by estrogen and progesterone receptor expression in the dog. (United States)

    Cooke, P S; Borsdorf, D C; Ekman, G C; Doty, K F; Clark, S G; Dziuk, P J; Bartol, F F


    During neonatal and juvenile life, mammalian uteri undergo extensive structural and functional changes, including uterine gland differentiation and development. In sheep and mice, inhibition of neonatal uterine gland development induced by progestin treatment led to a permanent aglandular uterine phenotype and adult infertility, suggesting that this strategy might be useful for sterilizing dogs and other companion animals. The goal of this study was to define temporal patterns of adenogenesis (gland development), cell proliferation, and progesterone and estrogen receptor expression in uteri of neonatal and juvenile dogs as a first step toward determining whether neonatal progestin treatments might be a feasible contraceptive approach in this species. Uteri obtained from puppies at postnatal wk 1, 2, 4, 6, or 8 were evaluated histologically and immunostained for MKI67, a marker of cell proliferation, estrogen receptor-1, and progesterone receptor. Adenogenesis was under way at 1 wk of age, as indicated by the presence of nascent glands beginning to bud from the luminal epithelium, and rapid proliferation of both luminal epithelial and stromal cells. By Week 2, glands were clearly identifiable and proliferation of luminal, glandular, and stromal cells was pronounced. At Week 4, increased numbers of endometrial glands were evident penetrating uterine stroma, even as proliferative activity decreased in all cell compartments as compared with Week 2. Whereas gland development was most advanced at Weeks 6 to 8, luminal, glandular, and stromal proliferation was minimal, indicating that the uterus was nearly mitotically quiescent at this age. Both estrogen receptor-1 and progesterone receptor were expressed consistently in uterine stromal and epithelial cells at all ages examined. In summary, canine uterine adenogenesis was underway by 1 wk of age and prepubertal glandular proliferation was essentially complete by Week 6. These results provided information necessary to

  1. Influence of condensed sweet cream buttermilk on the manufacture, yield, and functionality of pizza cheese. (United States)

    Govindasamy-Lucey, S; Lin, T; Jaeggi, J J; Johnson, M E; Lucey, J A


    Compositional changes in raw and pasteurized cream and unconcentrated sweet cream buttermilk (SCB) obtained from a local dairy were investigated over 1 yr. Total phospholipid (PL) composition in SCB ranged from 0.113 to 0.153%. Whey protein denaturation in pasteurized cream over 1 yr ranged from 18 to 59%. Pizza cheese was manufactured from milk standardized with condensed SCB (approximately 34.0% total solids, 9.0% casein, 17.8% lactose). Effects of using condensed SCB on composition, yield, PL recovery, and functional properties of pizza cheese were investigated. Cheesemilks were prepared by adding 0, 2, 4, and 6% (wt/wt) condensed SCB to part-skim milk, and cream was added to obtain cheesemilks with approximately 11.2 to 12.7% total solids and casein:fat ratio of approximately 1. Use of condensed SCB resulted in a significant increase in cheese moisture. Cheese-making procedures were modified to obtain similar cheese moisture contents. Fat and nitrogen recoveries in SCB cheeses were slightly lower and higher, respectively, than in control cheeses. Phospholipid recovery in cheeses was below 40%. Values of pH and 12% trichloro-acetic acid-soluble nitrogen were similar among all treatments. Cheeses made from milk standardized with SCB showed less melt and stretch than control cheese, especially at the 4 and 6% SCB levels. Addition of SCB significantly lowered free oil at wk 1 but there were no significant differences at wk 2 and 4. Use of SCB did not result in oxidized flavor in unmelted cheeses. At low levels (e.g., 2% SCB), addition of condensed SCB improved cheese yield without affecting compositional, rheological, and sensory properties of cheese. PMID:16428615

  2. NIRS-based noninvasive cerebrovascular regulation assessment (United States)

    Miller, S.; Richmond, I.; Borgos, J.; Mitra, K.


    Alterations to cerebral blood flow (CBF) have been implicated in diverse neurological conditions, including mild traumatic brain injury, microgravity induced intracranial pressure (ICP) increases, mild cognitive impairment, and Alzheimer's disease. Near infrared spectroscopy (NIRS)-measured regional cerebral tissue oxygen saturation (rSO2) provides an estimate of oxygenation of the interrogated cerebral volume that is useful in identifying trends and changes in oxygen supply to cerebral tissue and has been used to monitor cerebrovascular function during surgery and ventilation. In this study, CO2-inhalation-based hypercapnic breathing challenges were used as a tool to simulate CBF dysregulation, and NIRS was used to monitor the CBF autoregulatory response. A breathing circuit for the selective administration of CO2-compressed air mixtures was designed and used to assess CBF regulatory responses to hypercapnia in 26 healthy young adults using non-invasive methods and real-time sensors. After a 5 or 10 minute baseline period, 1 to 3 hypercapnic challenges of 5 or 10 minutes duration were delivered to each subject while rSO2, partial pressure of end tidal CO2 (PETCO2), and vital signs were continuously monitored. Change in rSO2 measurements from pre- to intrachallenge (ΔrSO2) detected periods of hypercapnic challenges. Subjects were grouped into three exercise factor levels (hr/wk), 1: 0, 2:>0 and 10. Exercise factor level 3 subjects showed significantly greater ΔrSO2 responses to CO2 challenges than level 2 and 1 subjects. No significant difference in ΔPETCO2 existed between these factor levels. Establishing baseline values of rSO2 in clinical practice may be useful in early detection of CBF changes.

  3. Performance and metabolic responses of Holstein calves to supplemental chromium in colostrum and milk. (United States)

    Ghorbani, A; Sadri, H; Alizadeh, A R; Bruckmaier, R M


    Twenty-two newborn Holstein female calves (BW = 39.7 ± 0.40 kg) were used to investigate the effects of chromium-l-methionine (Cr-Met) supplementation of colostrum for 3d after birth and mature milk up to wk 8 on feed intake, growth performance, health status, and metabolic and endocrine traits. Calves were randomly assigned to 2 groups, each consisting of 11 animals: 1) control and 2) 0.03 mg of supplemental Cr/kg of BW(0.75). Body weight, height at withers, and hearth girth were measured weekly. Dry matter intake, rectal temperature, fecal score, and respiratory score were recorded daily. Blood samples were collected at 12, 24, and 72 h after birth, and then every week up to 8 wk. Chromium did not affect mean body weight, dry matter intake, and withers height, but it increased hearth girth and average daily gain, tended to increase final BW, and decreased feed conversion ratio. Respiration rate increased and fecal score decreased with Cr, and rectal temperature tended to decrease with Cr. No Cr × time interactions were observed for performance and health status results except for fecal score. Blood glucose, insulin, insulin-to-glucose ratio, insulin-like growth factor-I, total protein, and triiodothyronine were not affected, whereas blood β-hydroxybutyrate, nonesterified fatty acids, cholesterol, cortisol, and thyroxin were affected by Cr supplementation. Supplemental Cr-Met decreased blood β-hydroxybutyrate at 72 h and in wk 1, 3, 4, 5, and 6 and decreased blood nonesterified fatty acids at 12h and in wk 3, 4, and 5 after birth. Blood cholesterol decreased in all sampling times, except for 12h and wk 7. Chromium decreased blood cortisol at 24h and in wk 2, 4, and 8. In conclusion, the present results demonstrate the beneficial effects of colostrum and milk supplementation with Cr to improve the performance and metabolic status of newborn calves. PMID:22863099

  4. Evaluation of feeding various sources of distillers dried grains with solubles in non-feed-withdrawal molt programs for laying hens. (United States)

    Bland, Kelly; Utterback, Pam; Koelkebeck, Ken; Parsons, Carl


    An experiment was conducted using 588 Hy-Line W-36 hens (68 wk of age) to evaluate if laying hens can be successfully molted by ad libitum feeding various levels of 3 sources of distillers dried grains with solubles (DDGS). Treatment 1 consisted of a 47% corn (C):47% soy hulls (SH) molt diet (C:SH) fed for 28 d (positive control). Treatments 2, 3, and 4 were molt diets containing 94% DDGS from the 3 sources fed for 28 d. Treatments 5, 6, and 7 were 32% C: 42% SH: 20% DDGS, from each of the 3 DDGS sources, also fed for 28 d. At the end of the 28-d molt period, all hens were fed a 16% CP corn-soybean meal layer diet. Body weight loss during the molt period was significantly greater (P hens fed the C:SH diet (26%) than hens fed the diets containing DDGS, and the reduction in BW loss varied among DDGS sources. Feed intake was lower (P Hens fed the C:SH diet had egg production near 0% during the last 3 wk of the molt period. Hens on the other treatments did not have mean egg production below 17% during the molt period (wk 1 to 4), and the reduction in egg production varied among DDGS sources. Postmolt hen-day egg production (5-41 wk) did not significantly differ among treatments; however, egg mass and egg specific gravity were generally reduced (P hens fed the 94% DDGS molt diets compared with hens fed the C:SH diet. This study showed that molt and postmolt performance responses varied among DDGS sources; however, none of the molt diets containing 20 to 94% DDGS yielded molt period reductions in BW or egg production similar to a 47% C: 47% SH diet. PMID:24879692

  5. Physical Activity Patterns During Pregnancy in a Sample of Portuguese Women: A Longitudinal Prospective Study (United States)

    Santos, Paula Clara; Abreu, Sandra; Moreira, Carla; Santos, Rute; Ferreira, Margarida; Alves, Odete; Moreira, Pedro; Mota, Jorge


    Background Physical activity (PA) patterns during pregnancy have not been explored in depth and most previous studies lack assessment of variables such as type, frequency, duration and intensity of activity. Objectives This study had two goals: 1) to analyze PA patterns during pregnancy according to weekly time spent on different types of activity; and 2) to determine women’s perception about health care providers regarding PA advisement during pregnancy. Patients and Methods A longitudinal prospective study was carried out with a 118-pregnant women cohort. Participants were evaluated during all trimesters. Self-reported questionnaires were used to collect personal and obstetric data. Type, duration and frequency of PA were evaluated using the pregnancy physical activity questionnaire (PPAQ) and intensity levels were calculated. Repeated measure analysis of variance was performed to determine differences between trimesters, and Wilcoxon signed-rank test was performed when appropriate. Results A decrease in values of self-reported PA (MET.h.wk-1) was found from the first to the second and the first to the third trimester of pregnancy, respectively; total (270.91 vs 220.54 vs 210.35; P sport activities. Swimming was the most reported organized PA, reaching its highest proportion (12.7%) in the second trimester. Prenatal exercise classes were reported by 39.8% of women during the 3rd trimester. Pregnant women reported that PA was recommended by health professionals: 53.9% in the 1st trimester, 70.4% in the 2nd trimester and 56.8% in the 3rd trimester. Conclusions Self-reported PA decreased, especially from the first to the second trimester, in total, light and moderate intensity. Women spent most of their weekly time on domestic, occupational and leisure activities, except sport activities. There are some health care providers that do not recommend physical activity during pregnancy. PMID:27247788

  6. Consumption of purple sweet potato leaves modulates human immune response: T-lymphocyte functions, lytic activity of natural killer cell and antibody production

    Institute of Scientific and Technical Information of China (English)

    Chiao-Ming Chen; Sing-Chung Li; Ya-Ling Lin; Ching-Yun Hsu; Ming-Jer Shieh; Jen-Fang Liu


    AIM: To study the immunological effects of physiological doses of purple sweet potato leaves (PSPL).METHODS: The randomized crossover study (two periods,each lasting for 2 wk) involved 16 healthy non-smoking adults of normal weight. The 6-wk study consisted of a run-in (wk 1) PSPL diet (daily consumption of 200 g PSPL) or a control diet (low polyphenols, with the amount of carotenoids adjusted to the same level as that of PSPL) (wk 2-3), washout diet (wk 4), and switched diet (wk 5-6). Fasting blood was collected weekly in the morning. T-lymphocyte function was assessed via the proliferation and secretion of immunoreactive cytokines.Salivary IgA secretion and the specific cytotoxic activities of cytotoxic T lymphocytes and natural killer (NK) cells were determined.RESULTS: The plasma β-carotene level increased with time in both groups, while the plasma polyphenol level decreased in the control group, and no significant difference was detected between the two groups.Although plasma polyphenol levels did not significantly increase in the PSPL group at the end of the study, they were significantly elevated in urine. PSPL consumption produced a significant increase in proliferation responsiveness of peripheral blood mononuclear cells (PBMC) and their secretion of immunoreactive IL-2 and IL-4. As well, lytic activity in NK cells was elevated in a time-dependent fashion. Salivary TgA secretion significantly decreased in control group after 2 wk, and returned to baseline following dietary switch to PSPL.CONCLUSION: Consumption of PSPL modulates various immune functions including increased proliferation responsiveness of PBMC, secretion of cytokines IL-2 and IL-4, and the lytic activity of NK cells. The responsible determinants of PSPL remain to be elucidated, as does the biological significance of the present observations.

  7. Skeletal Adaptations to Different Levels of Eccentric Resistance Following Eight Weeks of Training (United States)

    English, Kirk L.; Loehr, James A.; Lee, Stuart M. C.; Maddocks, Mary J.; Laughlin, Mitzi S.; Hagan, R. Donald


    Coupled concentric-eccentric resistive exercise maintains bone mineral density (BMD) during bed rest and aging. PURPOSE: We hypothesized that 8 wks of lower body resistive exercise training with higher ratios of eccentric to concentric loading would enhance hip and lumbar BMD. METHODS: Forty untrained male volunteers (34.9+/-7.0 yrs, 80.9+/-9.8 kg, 178.2+/-7.1 cm; mean+/-SD) were matched for leg press (LP) 1-Repetition Maximum (1-RM) strength and randomly assigned to one of 5 training groups. Concentric load (% 1-RM) was constant across groups, but each group trained with different levels of eccentric load (0, 33, 66, 100, or 138% of concentric) for all training sessions. Subjects performed a periodized supine LP and heel raise (HR) training program 3 d wk-1 for 8 wks using a modified Agaton Fitness System (Agaton Fitness AB, Boden, Sweden). Hip and lumbar BMD (g/sq cm) was measured in triplicate pre- and post-training using DXA (Hologic Discovery ). Pre- and post-training means were compared using the appropriate ANOVA and Tukey's post hoc tests. Within group pre- to post-training BMD was compared using paired t-tests with a Bonferroni adjustment. RESULTS: There was a main effect of training on L1, L2, L3, L4, total lumbar, and greater trochanter BMD, but there were no differences between groups. CONCLUSION: Eights wks of lower body resistive exercise increased greater trochanter and lumbar BMD. Inability to detect group differences may have been influenced by a potentially osteogenic vibration associated with device operation in the 0, 33, and 66% groups.


    Directory of Open Access Journals (Sweden)

    Olga Gonchar


    Full Text Available The aim of the present study was to examine the influence of intermittent hypoxia at rest and in combination with long-term high-intensity swimming exercise on lipid peroxidation and antioxidant defense system adaptation in skeletal muscles differing in fiber type composition. High-intensity chronic exercise was performed as swimming training with load that corresponded to ~ 75 % VO2max (30 min·day-1, 5 days·wk-1, for 4 wk. Intermittent hypoxic training (IHT consisted of repeated episodes of hypoxia (12%O2, 15 min, interrupted by equal periods of recovery (5 sessions/day, for 2 wk. Sessions of IHT were used during the first two weeks and during the last two weeks of chronic exercise. Oxidative (red gastrocnemius and soleus, mix and glycolytic (white gastrocnemius muscles were sampled. Our results indicated that high-intensity swim training in combination with sessions of IHT induced more profound antioxidative adaptations in skeletal muscles than the exercise training only. This adaptation has muscle fiber type specificity and is reflected in significantly elevated superoxide dismutase and catalase activities in highly oxidative muscle only. Training adaptation of GSH system (reduced glutathione content, activities of glutathione reductase, glutathione peroxidase, NADPH-supplying enzyme glucose-6-phosphate dehydrogenase occurred both in slow- and fast-twitch muscles. However, this process was more effective in oxidative muscles. IHT attenuated the increase in TBARS content induced by high-intensity swimming training. The test on exercise tolerance demonstrated a significant elevation of the swimming time to exhaustion after IHT at rest and after IHT in conjunction with high-intensity exercise in comparison with untrained and chronically exercised rats. These results confirmed that sessions of IHT might improve exercise tolerance and increase maximal work capacity

  9. Effect of anabolic steroids on overloaded and overloaded suspended skeletal muscle. (United States)

    Tsika, R W; Herrick, R E; Baldwin, K M


    The purpose of this study was to ascertain whether anabolic steroids act synergistically with functional overload in terms of increasing muscle weight and subcellular protein content of normal overloaded and suspended overloaded rodent plantaris muscle. Female rats were randomly assigned to six groups (7 rats/group) for 6 wk: 1) normal control (NC), 2) overload (OV), 3) overload steroid (OV-S), 4) normal suspended (N-SUS), 5) overload suspended (OV-SUS), and 6) overload suspended steroid (OV-SUS-S). Rats receiving anabolic steroid were administered 0.3 mg nandrolone decanoate (Deca-Durabolin) per day. Relative to control values, overload induced 1) sparing of muscle weight of the OV-SUS group as well as larger absolute and normalized (mg muscle/g body wt) muscle weight of the OV group (P less than 0.05), 2) greater protein content (mg/muscle, P less than 0.05), and 3) an increased relative expression of slow myosin in both the OV and OV-SUS groups (P less than 0.05). Although anabolic steroid treatment of overload animals (OV-S) did not alter further the pattern of response of any parameter analyzed for the OV group, it did induce larger absolute and normalized muscle weight (P less than 0.05) as well as a greater protein content (mg/muscle, P less than 0.05) of the OV-SUS-S group compared with control values. However, anabolic steroid treatment did not alter the pattern of isomyosin expression observed in the overload (OV-S vs. OV) or overload suspended (OV-SUS-S vs. OV-SUS) groups.(ABSTRACT TRUNCATED AT 250 WORDS) PMID:3693242

  10. Effect of anabolic steroids on skeletal muscle mass during hindlimb suspension. (United States)

    Tsika, R W; Herrick, R E; Baldwin, K M


    The efficacy of anabolic steroid treatment [0.3 or 0.9 mg nandrolone decanoate (Deca-Durabolin) per day] was examined in the context of sparing rodent fast-twitch plantaris and slow-twitch soleus muscle weight, sparing subcellular protein, and altering isomyosin expression in response to hindlimb suspension. Female rats were assigned to four groups (7 rats/group for 6 wk): 1) normal control (NC), 2) normal steroid (NS), 3) normal suspension (N-SUS), and 4) suspension steroid (SUS-S). Compared with control values for the plantaris and soleus muscles, suspension induced 1) smaller body and muscle weight (P less than 0.05), 2) losses in myofibril content (mg/muscle, P less than 0.05), and 3) shifts in the relative expression (expressed as %of total isomyosin) of isomyosins which favored lesser slow myosin and greater fast myosin isotypes (P less than 0.05). Steroid treatment of suspended animals (SUS-S vs. N-SUS) partially spared body and muscle weight (P less than 0.05) and spared plantaris but not soleus myofibril content (mg/muscle, P less than 0.05). However, steroid treatment did not modify the isomyosin pattern induced by suspension. In normal rats (NS vs. NC), steroid treatment enhanced body and plantaris muscle weight but not soleus weight (P less than 0.05) and did not alter isomyosin expression in either muscle type. Collectively these data suggest that in young female rats anabolic steroids 1) enhance the body weight and the weight of a fast-twitch ankle extensor in normal rats, 2) ameliorate the loss in body weight, fast-twitch muscle weight and protein content and slow-twitch muscle weight associated with hindlimb suspension.(ABSTRACT TRUNCATED AT 250 WORDS) PMID:2961725

  11. Comparative physiology of radish populations with differential sensitivity to O3 and SO2

    International Nuclear Information System (INIS)

    Radish plants (Raphanus sativus L. cv Cherry Belle) were exposed to 0.10 μl l-1 ozone (O3) or 0.50 μl l-1 sulfur dioxide (SO2) for 4 h d-1, 3 d wk-1 for 3 weeks. From these fumigated plants, individuals were selected that were resistant or sensitive to these pollutants. The selected plants were used as parental material in a breeding program to produce lines differing in resistance to O3 and SO2. Non-selected (NS) plants from the original populations served as controls. Plants selected for O3 resistance (O3R) weighed significantly more than either plants selected for sensitivity to O3 (O3S) or NS plants when exposed to either O3 or SO2. Plants selected for resistance or sensitivity to SO2 generally had biomass production similar to that of NS plants. Ozone fumigations tended to decrease free sugar concentrations in leaves at early stages of growth in both O3R and O3S plants, and caused some accumulations of carbohydrates during late stages of growth in O3S plants. Allocation of 14C was significantly lower to hypocotyls and roots of O3 fumigated O3S plants. Allocation to hypocotyls of O3R plants was not affected, although both O3R and O3S plant groups had lower photosynthetic rates due to O3 fumigation. Ozone did not significantly affect chlorophyll concentrations in leaves of either sensitive or resistant plants, nor was the time of new leaf production affected by fumigation

  12. Red/near-infrared light-emitting diode therapy for traumatic brain injury (United States)

    Naeser, Margaret A.; Martin, Paula I.; Ho, Michael D.; Krengel, Maxine H.; Bogdanova, Yelena; Knight, Jeffrey A.; Yee, Megan K.; Zafonte, Ross; Frazier, Judith; Hamblin, Michael R.; Koo, Bang-Bon


    This invited paper reviews our research with scalp application of red/near-infrared (NIR) light-emitting diodes (LED) to improve cognition in chronic, traumatic brain injury 1. Application of red/NIR light improves mitochondrial function (especially hypoxic/compromised cells) promoting increased ATP, important for cellular metabolism. Nitric oxide is released locally, increasing regional cerebral blood flow. Eleven chronic, mTBI participants with closed-head injury and cognitive dysfunction received 18 outpatient treatments (MWF, 6 Wks) starting at 10 Mo. to 8 Yr. post-mTBI (MVA, sports-related, IED blast injury). LED therapy is non-invasive, painless, non-thermal (FDA-cleared, non-significant risk device). Each LED cluster head (2.1" diameter, 500mW, 22.2mW/cm2) was applied 10 min (13J/cm2) to 11 scalp placements: midline, from front-to-back hairline; and bilaterally on dorsolateral prefrontal cortex, temporal, and parietal areas. Testing performed pre- and post-LED (+1 Wk, 1 and 2 Mo post- 18th treatment) showed significant linear trend for LED effect over time, on improved executive function and verbal memory. Fewer PTSD symptoms were reported. New studies at VA Boston include TBI patients treated with transcranial LED (26J/cm2); or treated with only intranasal red, 633nm and NIR, 810nm diodes placed into the nostrils (25 min, 6.5mW, 11.4J/cm2). Intranasal LEDs are hypothesized to deliver photons to hippocampus. Results are similar to Naeser et al. (2014). Actigraphy sleep data show increased sleep time (average, +1 Hr/night) post-18th transcranial or intranasal LED treatment. LED treatments may be self-administered at home (Naeser et al., 2011). A shamcontrolled study with Gulf War Illness Veterans is underway.

  13. Effect of omeprazole and domperidone on adult asthmatics with gastroesophageal reflux

    Institute of Scientific and Technical Information of China (English)

    Bhavneesh Sharma; Manisha Sharma; Mradul Kumar Daga; Gopal Krishan Sachdev; Elliott Bondi


    AIM: To study the effect of combined omeprazole(Ome) and domperidone(Dom) therapy on asthma symptoms and pulmonary function in asthmatics with gastroesoph ageal reflux.METHODS: We selected 198 asthmatics with gastro esophageal reflux diagnosed by 24-h esophageal pH moni toring to receive Ome 20 mg twice daily and Dom 10 mg three times daily or placebo for 16 wk (1:1 double-blind randomization). Spirometry was done at baseline and af ter 16 wk of treatment. The primary outcome measures were: mean daily daytime and nighttime asthma symp tom scores. Mean daily reflux symptom scores, albuterol use as rescue medication (number of puffs), daytime and nighttime peak expiratory flow rate (PEFR), postbroncho dilator forced expiratory volume in 1 second (FEV1) and postbronchodilator forced vital capacity (FVC) were sec ondary outcome measures.RESULTS: Comparison of mean change from baseline between antireflux therapy and placebo groups revealed significant reduction in daytime asthma symptom score (17.4% vs 8.9 %), nighttime asthma symptom score (19.6% vs 5.4%), reflux symptom score (8.7% vs 1.6%) and rescue medication use (23.2% vs 3.1%) after antire flux therapy compared to mean change in placebo group (P < 0.001). There was significant improvement in morn ing PEFR (7.9% vs 0.2%), evening PEFR (9.8% vs 0.5%), FEV1 (11.1% vs 3.78%) and FVC (9.3% vs 1.52%) in the antireflux therapy group compared to placebo on comparing the mean change from baseline after 16 wk (P < 0.01).CONCLUSION: Combined therapy with Ome and Dom in adult asthmatics with gastroesophageal reflux may be beneficial by reducing asthma symptoms, rescuing medi cation use, and improving pulmonary function.

  14. Performance and Meat Quality of Local Sheep Administered with Feed Additive Containing Probiotic and Organic Mineral Complex

    Directory of Open Access Journals (Sweden)

    H. Herdian


    Full Text Available The experiment was conducted to evaluate the effect of probiotic and organic mineral complex (POMC administration on performance and meat quality of local sheep. In this study, 6 sheep with an average initial body weight of 12.67±0.81 kg were arranged in a completely randomized design with 2 treatments and 3 replications. The treatments were T0: basal diet (control and T1: basal diet + POMC. The basal diet consisted of forage and concentrate (60:40 with dry matter of feed intake as much as 5% of body weight. Dose of POMC used in this study was 15 g/d/head. The POMC contained lactic acid bacteria, i.e. Lactobacillus sp. (1x108 cfu/g and S. cereviseae incorporated with micromineral Co (2 ppm, Cu (100 ppm, Fe (2.5 ppm, I (110 ppm and Mn (100 ppm. The experimental period lasted for 11 wk (1 wk for adaptation and 10 wk for data collection. Data were analyzed using t-Student statistical test to compare the treatments. The results showed that POMC administration did not affect the body weight gain (BWG (7.46 kg compared to control (7.13 kg while concentrate consumption (26.9 kg of POMC was lower than the control (28.6 kg. Meanwhile POMC administration did not affect the meat quality (pH, moisture, cooking loss and tenderness, whereas the meat cholesterol was lower (34.25 mg/100g than the control (38.87 mg/100g. It is concluded that administration of probiotic lactic acid bacteria combined with organic minerals decreases concentrate consumption and thereby potentially increases the animals energy utilization efficiency. In addition, the treatment also decreases the meat cholesterol content of local sheep.

  15. Intravaginal Lactic Acid Bacteria Modulated Local and Systemic Immune Responses and Lowered the Incidence of Uterine Infections in Periparturient Dairy Cows (United States)

    Deng, Qilan; Odhiambo, John F.; Farooq, Umar; Lam, Tran; Dunn, Suzanna M.; Ametaj, Burim N.


    The objective of this investigation was to evaluate whether intravaginal infusion of a lactic acid bacteria (LAB) cocktail around parturition could influence the immune response, incidence rate of uterine infections, and the overall health status of periparturient dairy cows. One hundred pregnant Holstein dairy cows were assigned to 1 of the 3 experimental groups as follows: 1) one dose of LAB on wk -2 and -1, and one dose of carrier (sterile skim milk) on wk +1 relative to the expected day of parturition (TRT1); 2) one dose of LAB on wk -2, -1, and +1 (TRT2), and 3) one dose of carrier on wk -2, -1, and +1 (CTR). The LAB were a lyophilized culture mixture composed of Lactobacillus sakei FUA3089, Pediococcus acidilactici FUA3138, and Pediococcus acidilactici FUA3140 with a cell count of 108-109 cfu/dose. Blood samples and vaginal mucus were collected once a week from wk -2 to +3 and analyzed for content of serum total immunoglobulin G (IgG), lipopolysaccharide-binding protein (LBP), serum amyloid A (SAA), haptoglobin (Hp), tumor necrosis factor (TNF), interleukin (IL)-1, IL-6, and vaginal mucus secretory IgA (sIgA). Clinical observations including rectal temperature, vaginal discharges, retained placenta, displaced abomasum, and laminitis were monitored from wk -2 to +8 relative to calving. Results showed that intravaginal LAB lowered the incidence of metritis and total uterine infections. Intravaginal LAB also were associated with lower concentrations of systemic LBP, an overall tendency for lower SAA, and greater vaginal mucus sIgA. No differences were observed for serum concentrations of Hp, TNF, IL-1, IL-6 and total IgG among the treatment groups. Administration with LAB had no effect on the incidence rates of other transition cow diseases. Overall intravaginal LAB lowered uterine infections and improved local and systemic immune responses in the treated transition dairy cows. PMID:25919010

  16. Effects of long-term aerobic exercise on EPOC. (United States)

    LeCheminant, J D; Jacobsen, D J; Bailey, B W; Mayo, M S; Hill, J O; Smith, B K; Donnelly, J E


    This study sought to determine the influence of 16 months of progressive aerobic exercise on excess postexercise oxygen consumption (EPOC) and the extent EPOC contributed to weight management. Twenty-five overweight/obese women and 16 overweight/obese men participated in a 16-month exercise program (moderate-intensity treadmill walking) that progressed across the first 26 weeks to 5 days.wk(-1), 45 min.session(-1), and 75% HRR. Three-hour EPOC was measured at baseline, 9 months, and 16 months by indirect calorimetry in response to an exercise session (treadmill walking), in which energy expenditure (EE) was estimated from the participant's previous 10 exercise sessions. For women, EPOC was 7.5 +/- 4.9, 9.6 +/- 7.6, and 6.5 +/- 6.5 L at baseline, 9 months, and 16 months, respectively (p > 0.05). For men, EPOC increased from baseline (11.8 +/- 6.8 L) to 9 months (13.5 +/- 8.6 L) (p < 0.05) with no further increase at 16 months (13.5 +/- 11.0 L). Change in EPOC was correlated with change in EE at 9 months (r = 0.65; p < 0.05) and 16 months (r = 0.58; p < 0.05) for men but not women. Progressive long-term exercise significantly influenced EPOC in overweight/obese men but not women. Change in volume of exercise likely explained the increase in energy expenditure during EPOC in men. EPOC contributed modestly to EE compared to the exercise itself. PMID:17879880

  17. 曲唑酮治疗焦虑症%Trazodone in treatment of anxiety disorders

    Institute of Scientific and Technical Information of China (English)

    吴恺; 胡夏生


    目的:观察曲唑酮对焦虑症的疗效.方法:选择符合CCMD-2-R诊断标准的焦虑症病例90例(实际完成87例:男性27例,女性60例),随机分3组,每组30例,分别用曲唑酮50~100 mg·d-1, po,阿普唑仑1.6 mg·d-1, po,劳拉西泮2 mg·d-1, po,共观察4 wk.结果:曲唑酮组的总有效率基本与其他2组相仿(P>0.05),仅wk 4优于阿普唑仑组(P<0.05).HAMA减分率与wk 1阿普唑仑组及各周劳拉西泮组差别无显著意义(P>0.05),但与wk 2,wk 4阿普唑仑组比较差别有显著和非常显著意义(P<0.05或P<0.01).药物副作用少而轻,以胃肠道不适和困倦感为主,仅3例分别出现头痛伴焦虑、头晕伴烦躁不安、焦虑症状加重.结论:曲唑酮是有效的抗焦虑药物,且副作用小.

  18. Improving feeding systems for sheep in a Mediterranean rain-fed cereals/livestock area of Morocco. (United States)

    Guessous, F; Rihani, N; Kabbali, A; Johnson, W L


    In rain-fed cereal-producing areas of Morocco, stubble and straw from barley and wheat and forage grazed from fallow lands are important feeds for sheep. Supplementation to maintain BW of ewes pregnant while grazing stubble, methods to improve utilization of straw, annual forage legumes to complement grazing of fallow land, and by-product feeds in diets for weaned lambs have been tested in collaborative research trials. Pregnant ewes grazing cereal stubble for 10 to 12 wk at a modest stocking rate and unsupplemented, or at a heavier stocking rate and supplemented after 5 wk, gained about 3 kg; most of the gain occurred in wk 1 to 4 due to intake of residual scattered grain. Following the stubble-grazing period (coincident with late gestation), straw plus an energy supplement alone resulted in low lamb birth weight and survivability. Urea treatment increased N content of straw and holds potential. After the onset of winter rains, self-reseeding annual legume (Medicago spp.) pastures allowed lactating Timahdit ewes to gain 4 kg during 90 d postpartum. Their suckling lambs gained 260 g/d, thus doubling the weight of lamb weaned per ewe compared with those grazing unimproved fallow. Confined growing lambs fed diets with 40% sugarbeet pulp or 20% carob meal gained more than 200 g/d, reducing the time needed to reach market weight. These results demonstrate that marked improvements in performance and efficiency of sheep in Mediterranean rain-fed cereal/livestock systems are possible using locally available resources. PMID:2687218

  19. Increased hypolipidemic benefits of cis-9, trans-11 conjugated linoleic acid in combination with trans-11 vaccenic acid in a rodent model of the metabolic syndrome, the JCR:LA-cp rat

    Directory of Open Access Journals (Sweden)

    Ruth Megan R


    Full Text Available Abstract Background Conjugated linoleic acid (cis-9, trans-11 CLA and trans-11 vaccenic acid (VA are found naturally in ruminant-derived foods. CLA has been shown to have numerous potential health related effects and has been extensively investigated. More recently, we have shown that VA has lipid-lowering properties associated with reduced hepatic lipidogenesis and chylomicron secretion in the JCR:LA-cp rat. The aim of this study was to evaluate potential additional hypolipidemic effects of purified forms of CLA and VA in an animal model of the metabolic syndrome (the JCR:LA-cp rat. Methods Twenty four obese JCR:LA-cp rats were randomized and assigned to one of three nutritionally adequate iso-caloric diets containing 1% w/w cholesterol and 15% w/w fat for 16 wk: 1 control diet (CD, 2 1.0% w/w cis-9, trans-11 CLA (CLA, 3 1.0% w/w VA and 1% w/w cis-9, trans-11 CLA (VA+CLA. Lean rats were fed the CD to represent normolipidemic conditions. Results Fasting plasma triglyceride (TG, total cholesterol and LDL-cholesterol concentrations were reduced in obese rats fed either the CLA diet or the VA+CLA diet as compared to the obese control group (p Conclusion We demonstrate that the hypolipidemic effects of chronic cis-9, trans-11 CLA supplementation on circulating dyslipidemia and hepatic steatosis are enhanced by the addition of VA in the JCR:LA-cp rat.

  20. Clinical research of limbal relaxing incision during implantable collamer lens surgery

    Directory of Open Access Journals (Sweden)

    Zhen Li


    Full Text Available AIM: To evaluate the efficacy and safety of limbal relaxing incision(LRIfor correcting corneal astigmatism during implantable collamer lens(ICLsurgery.METHODS: A total of 185 eyes of 105 patients with high myopia and corneal keratometric astigmatism were included in the study. ICL surgery with concomitant relaxing incision was performed in 105 eyes of 60 patients in LRIs group(Group A. Eighty eyes of 45 patients only underwent ICL surgery were in control group(Group B. All patients undergone ophthalmic examination that included uncorrected visual acuity(UCVA, best-corrected visual acuity(BCVA, Pentacam analysis system to observe the changes of corneal astigmatism before and 1wk, 1 and 3mo after surgery.RESULTS: Respectively comparing UCVA between two groups in 1 and 3mo postoperatively, the P values were considered statistically significant(PP values were considered no statistically significant(P>0.05. Preoperative corneal astigmatism was 1.52±0.55D in group A and 1.48±0.57D in group B, there was no statistically significant difference(P>0.05. One week postoperatively, the astigmatism was 0.55±0.41D in group A and 1.20±0.48D in group B. One month postoperatively, the astigmatism was 0.60±0.38D in group A and 0.93±0.47D. Three months postoperatively, the astigmatism was 0.51±0.32D in group A and 0.96±0.40D in group B. The difference between the two groups were statistically significant(PPPCONCLUSION: LRIs performed during ICL surgery appeared to be an effective and safer procedure to reduce pre-existing corneal astigmatism and improve UCVA as well as the visual quality.

  1. Impact of chemically amended pig slurry on greenhouse gas emissions, soil properties and leachate. (United States)

    O' Flynn, Cornelius J; Healy, Mark G; Lanigan, Gary J; Troy, Shane M; Somers, Cathal; Fenton, Owen


    The effectiveness of chemical amendment of pig slurry to ameliorate phosphorus (P) losses in runoff is well studied, but research mainly has concentrated only on the runoff pathway. The aims of this study were to investigate changes to leachate nutrient losses, soil properties and greenhouse gas (GHG) emissions due to the chemical amendment of pig slurry spread at 19 kg total phosphorus (TP), 90 kg total nitrogen (TN), and 180 kg total carbon (TC) ha(-1). The amendments examined were: (1) commercial grade liquid alum (8% Al2O3) applied at a rate of 0.88:1 [Al:TP], (2) commercial-grade liquid ferric chloride (38% FeCl3) applied at a rate of 0.89:1 [Fe:TP] and (3) commercial-grade liquid poly-aluminium chloride (PAC) (10% Al2O3) applied at a rate of 0.72:1 [Al:TP]. Columns filled with sieved soil were incubated for 8 mo at 10 °C and were leached with 160 mL (19 mm) distilled water wk(-1). All amendments reduced the Morgan's phosphorus and water extractable P content of the soil to that of the soil-only treatment, indicating that they have the ability to reduce P loss in leachate following slurry application. There were no significant differences between treatments for nitrogen (N) or carbon (C) in leachate or soil, indicating no deleterious impact on reactive N emissions or soil C cycling. Chemical amendment posed no significant change to GHG emissions from pig slurry, and in the cases of alum and PAC, reduced cumulative N2O and CO2 losses. Chemical amendment of land applied pig slurry can reduce P in runoff without any negative impact on nutrient leaching and GHG emissions. Future work must be conducted to ascertain if more significant reductions in GHG emissions are possible with chemical amendments. PMID:23850764

  2. Utilization of roughages and concentrates relative to that of milk replacer increases strongly with age in veal calves. (United States)

    Berends, H; van den Borne, J J G C; Mollenhorst, H; van Reenen, C G; Bokkers, E A M; Gerrits, W J J


    We aimed to investigate the feeding values of milk replacer (MR), roughage, and concentrates for veal calves in a paired-gain setting, thus avoiding any prior assumptions in feeding values and major differences in nutrient intakes. One hundred sixty male Holstein-Friesian calves at 2 wk of age and 45 ± 0.2 kg of body weight (BW) were included in the experiment. Calves were allocated to pens (5 calves per pen) and pens were randomly assigned to 1 of 4 solid feed (SF) levels: SF1, SF2, SF3, or SF4, respectively, and to 1 of 2 roughage-to-concentrate (R:C) ratios: 20:80 or 50:50. An adaptation period from wk 1 to 10 preceded the experimental period (wk 11 to 27). Total dry matter (DM) intake from SF was targeted to reach 20, 100, 180, and 260 kg of DM for SF1 to SF4, respectively, during the 16-wk experimental period, and increased with preplanned, equal weekly increments. Roughage was composed of 50% corn silage and 50% chopped wheat straw based on DM. The quantity of MR provided was adjusted every 2 wk based on BW to achieve similar targeted rates of carcass gain across treatments. The reduction in MR provided (in kg of DM) to realize equal rates of carcass gain with inclusion of SF (in kg of DM) differed between the R:C ratio of 50:50 (0.41 kg of MR/kg of SF) and the R:C ratio of 20:80 (0.52 kg of MR/kg of SF). As carcass gain unintentionally increased with SF intake, the paired-gain objective was not fully achieved. When adjusted for realized rates of carcass gain, calves fed an R:C ratio of 20:80 still required 10% less MR than calves fed an R:C ratio of 50:50 for equal rates of carcass gain, indicating that the utilization of SF for gain increased with concentrate inclusion. Averaged for the 16-wk experimental period, the feeding value of MR relative to that of concentrates and roughages was close to that predicted based on their respective digestible energy contents. Nevertheless, the feeding value of SF relative to that of MR increased substantially with age

  3. Mustard bran in lactating dairy cow diets. (United States)

    Maiga, H A; Bauer, M L; Dahlen, C R; Badaruddin, M; Scholljegerdes, E J


    Two trials using lactating Holstein cows were conducted to evaluate effects of a diet containing oriental mustard bran on dry matter intake (DMI), milk production, milk components, and organoleptic properties. In experiment 1, 34 lactating cows (24 multiparous and 10 primiparous; days in milk ≥ 50 d) were used in a switchback design to determine the lactational response and organoleptic quality of milk when the diet contained 8% oriental mustard bran (MB) versus a control diet (CON). Mustard bran replaced a portion of soybean meal and all the beet pulp in the CON diet. Milk yields were greater for cows fed the MB diet; however, no differences were found in DMI, 3.5% fat- (FCM) or solids-corrected milk. Milk components and components production were not affected by treatment. Milk organoleptic qualities were not affected by diet. In experiment 2, 22 lactating cows (16 multiparous and 6 primiparous; days in milk ≥ 21 d) were assigned randomly within parity to receive MB or CON from wk 4 to 19 postpartum in a randomized complete block design. Cows were fed CON wk 1 to 3 postpartum. The MB diet contained the same ingredients as the CON, except sunflower seed and a portion of soybean meal were replaced with mustard bran. Milk and components data were collected during wk 3 postpartum and used as covariates to adjust treatment means. Intake was greater for cows fed the MB diet; however, daily milk, 3.5% FCM, and solids-corrected milk yields were not different between diets. Milk components and component yields were not affected by treatment. Milk urea concentration was less for cows fed the MB diet. Although cows fed the MB diet had greater DMI, this was not translated into a higher milk 3.5% FCM/DMI production efficiency ratio. During experiment 2, many cows fed MB experienced minor to severe hemolysis with bloody urine. This hemolysis believed to be caused by the S-methyl-cysteine sulfoxide contained in mustard bran could have affected milk production efficiency

  4. Body condition score at calving affects systemic and hepatic transcriptome indicators of inflammation and nutrient metabolism in grazing dairy cows. (United States)

    Akbar, H; Grala, T M; Vailati Riboni, M; Cardoso, F C; Verkerk, G; McGowan, J; Macdonald, K; Webster, J; Schutz, K; Meier, S; Matthews, L; Roche, J R; Loor, J J


    Calving body condition score (BCS) is an important determinant of early-lactation dry matter intake, milk yield, and disease incidence. The current study investigated the metabolic and molecular changes induced by the change in BCS. A group of cows of mixed age and breed were managed from the second half of the previous lactation to achieve mean group BCS (10-point scale) that were high (HBCS, 5.5; n=20), medium (MBCS, 4.5; n=18), or low (LBCS, 3.5; n=19). Blood was sampled at wk -4, -3, -2, 1, 3, 5, and 6 relative to parturition to measure biomarkers of energy balance, inflammation, and liver function. Liver was biopsied on wk 1, 3, and 5 relative to parturition, and 10 cows per BCS group were used for transcript profiling via quantitative PCR. Cows in HBCS and MBCS produced more milk and had greater concentrations of nonesterified fatty acids and β-hydroxybutyrate postpartum than LBCS. Peak concentrations of nonesterified fatty acids and β-hydroxybutyrate and greater hepatic triacylglycerol concentrations were recorded in HBCS at wk 3. Consistent with blood biomarkers, HBCS and MBCS had greater expression of genes associated with fatty acid oxidation (CPT1A, ACOX1), ketogenesis (HMGCS2), and hepatokines (FGF21, ANGPTL4), whereas HBCS had the lowest expression of APOB (lipoprotein transport). Greater expression during early lactation of BBOX1 in MBCS and LBCS suggested greater de novo carnitine synthesis. The greater BCS was associated with lower expression of growth hormone/insulin-like growth factor-1 signaling axis genes (GHR1A, IGF1, and IGFALS) and greater expression of gluconeogenic genes. These likely contributed to the higher milk production and greater gluconeogenesis. Despite greater serum haptoglobin around calving, cows in HBCS and MBCS had greater blood albumin. Cows in MBCS, however, had a higher albumin:globulin ratio, probably indicating a less pronounced inflammatory status and better liver function. The marked decrease in expression of NFKB1

  5. Rugby-specific small-sided games training is an effective alternative to stationary cycling at reducing clinical risk factors associated with the development of type 2 diabetes: a randomized, controlled trial.

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    Amy E Mendham

    Full Text Available The present study investigated whether rugby small-sided games (SSG could be an effective alternative to continuous stationary cycling (CYC training at reducing clinical risk factors associated with the development of type 2 diabetes mellitus (T2DM.Thirty-three middle-aged (48.6±6.6y, inactive men were randomized into a CYC (n=11, SSG (n=11, or control (CON, n=11 group. Participants trained 3d·wk(-1 for 8 weeks, while control participants maintained normal activity and dietary patterns. Exercise duration was matched between groups, which involved CYC or SSG (four quarters, interspersed with 2-min passive recovery. Both training programs were designed to induce similar internal loads of maximal heart rate (~80-85%HRmax and rating of perceived exertion. Pre- and post-intervention testing included dual-energy x-ray absorptiometry scan, graded exercise test, fasting 2 h oral glucose tolerance test and resting muscle biopsy. Western blotting was used to assess the content of skeletal muscle proteins associated with mitochondrial biogenesis and glucose regulation.Both CYC and SSG increased VO2 at 80%HRmax, and reduced glycated haemoglobin, glucose area under the curve (AUC; SSG, -2.3±2.4; CYC -2.2±1.6 mmol·L(1(120 min(1; p0.05. There were no differences within or between conditions for protein content of peroxisome proliferator-activated receptor gamma coactivator-1α, sirtuin-1, p53, glucose transporter-4, protein kinase AKT/PKB, myocyte enhancer factor 2A, mitochondrial transcription factor, nuclear respiratory factor (NRF-1, NRF-2 or mitochondrial complexes I-V (p>0.05.Rugby small-sided games is an effective alternative to continuous cycling for improving metabolic risk-factors associated with the prevention of T2DM. Despite such positive adaptations in clinical risk factors, there were no changes in the content of skeletal muscle proteins associated with glucose regulation and mitochondrial biogenesis.Australian New Zealand Clinical Trial

  6. The effects of a ration change from a total mixed ration to pasture on health and production of dairy cows. (United States)

    Schären, M; Jostmeier, S; Ruesink, S; Hüther, L; Frahm, J; Bulang, M; Meyer, U; Rehage, J; Isselstein, J; Breves, G; Dänicke, S


    In pasture-based dairy production systems, dairy cows often receive a silage- and concentrate-based ration [total mixed ration (TMR)] during wintertime and are gradually introduced to fresh herbage in spring. The present study aimed to investigate how the transition to this new nutritional situation influenced different production and health indicators. A 10-wk trial was performed in spring 2014, including 60 dairy cows of the German Holstein breed (166±23 d in milk, 23.5±3.7 kg of milk/d; means ± SD). The cows were divided into a pasture and a confinement group (PG and CG, respectively). The CG stayed on a TMR-based diet (35% corn silage, 35% grass silage, 30% concentrate; DM basis), whereas the PG was gradually transitioned from a TMR- to a pasture-based ration (wk 1=TMR-only, wk 2=3 h/d on pasture, wk 3 and 4=12 h/d on pasture, wk 5-10=pasture-only). A continuous grazing system was implemented on a ryegrass dominated pasture and temperature humidity indices were assessed based on continuous recording of temperature and humidity indoors as well as outdoors. Dry matter intake (DMI) from TMR, milk production, body weight (BW), and body condition score decreased as soon as the PG had partial access to pasture. Milk production and BW decreased even further in the first week on a full grazing ration, but thereafter BW increased again and milk production stabilized. The DMI estimation using the n-alkane method in wk 7 and 9 revealed an increase in DMI from pasture between the 2 time points and indicates an adaptation of grazing behavior and metabolism over several weeks. Increased serum β-hydroxybutyrate and fatty acids concentrations at several time points, as well as a continuous body condition score decrease during the whole course of the trial, indicate an energy deficit in the PG. A significant correlation between serum glucose concentrations and the temperature humidity indices was observed. An increase in serum and milk urea concentrations as well as an

  7. Effects of long-term supplementation of laying hens with high concentrations of cholecalciferol on performance and egg quality. (United States)

    Persia, M E; Higgins, M; Wang, T; Trample, D; Bobeck, E A


    There is current interest in increasing human vitamin D dietary intake without having to modify human eating habits. One method to increase human dietary vitamin D intake is to generate eggs with increased concentrations of vitamin D through high-concentration vitamin D feeding in the diets of laying hens. Although eggs can be produced with high concentrations of vitamin D, the consequences of these diets on hen performance and egg quality have not been validated. The objective of this research is to quantify the effects of high concentrations of cholecalciferol (D3) on laying hen performance and egg quality. Hy-Line W36 laying hens were placed on 1 of 5 experimental diets for 40 wk: 1) control (contained 2,200 IU of D3/kg of diet), 2) control + 7,500 IU of D3/kg of diet (9,700 IU of D3/kg of diet total), 3) control + 15,000 IU of D3/kg of diet (17,200 IU of D3/kg of diet total), 4) control + 22,500 IU of D3/kg of diet (24,700 IU of D3/kg of diet total), and 5) control + 100,000 IU of D3/kg of diet (102,200 IU of D3/kg of diet total). Egg production and hen mortality were monitored daily. Feed intake was determined weekly. Eggs were collected at predetermined points throughout the 40-wk period (19 to 58 wk of bird age) for assessment of egg weight, egg component weights, Haugh unit, yolk color score, specific gravity, egg mass, and feed efficiency. There were no consistent differences among the dietary treatments over the experimental period. Hens supplemented with up to 102,200 IU of D3/kg of diet resulted in no significant reductions in egg production, feed intake, feed efficiency, egg component weights, yolk color, Haugh units, and specific gravity in comparison with the control-fed hens (P > 0.05). These data suggest the addition of cholecalciferol to the diet of the laying hen at concentrations up to 102,200 IU of D3/kg of diet had no consistent negative effects on laying hen performance or egg quality. PMID:24135597

  8. Associations of subclinical hypocalcemia at calving with milk yield, and feeding, drinking, and standing behaviors around parturition in Holstein cows. (United States)

    Jawor, P E; Huzzey, J M; LeBlanc, S J; von Keyserlingk, M A G


    The objectives of this study were to describe the associations of subclinical hypocalcemia with milk yield, and feeding, drinking, and resting behavior during the period around calving. Blood was sampled within 24h of calving and analyzed for serum total calcium. Fifteen Holstein dairy cows were classified as having subclinical hypocalcemia (serum calcium concentration ≤ 1.8 mmol/L, without clinical milk fever) and were matched with 15 control cows (serum calcium concentration >1.8 mmol/L) based on parity and presence of other diseases. Daily feeding and drinking behavior were monitored using an electronic feeding system (Insentec, BV, Marknesse, the Netherlands) and summarized by week relative to calving (wk -3, -2, -1, +1, +2, and +3). Standing behavior was monitored from 7 d before until 7 d after calving using dataloggers. Daily milk yields were obtained for all cows up to 280 d in milk (DIM). These data were summarized by week for the first 4 wk of lactation to assess short-term differences in milk yield, and were summarized into 4-wk periods to assess long-term (280 DIM) differences in milk yield between groups. Cows with subclinical hypocalcemia produced, on average, 5.7 kg/d more milk during wk 2, 3, and 4 compared with control cows; however, only subclinically hypocalcemic cows in their third lactation sustained greater milk yields throughout 280 DIM. Despite greater milk yield during the weeks following calving, cows with subclinical hypocalcemia did not consume more water after calving and tended to have greater dry matter intake only during wk 2. However, these animals made fewer visits to the water bins during the first 2 wk after calving and tended to make fewer visits to the feed bins during wk 1 and 3, suggesting that they used these resources more efficiently. Dry matter intake was, on average, 1.7 kg/d greater during wk -2 and -1 among cows subsequently diagnosed with subclinical hypocalcemia compared with control cows but neither group was

  9. Effects of resistance training and protein supplementation on bone turnover in young adult women

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    Sinning Wayne E


    Full Text Available Abstract Background The strength of aging bone depends on the balance between the resorption and formation phases of the remodeling process. The purpose of this study was to examine the interaction of two factors with the potential to exert opposing influences on bone turnover, resistance exercise training and high dietary protein intake. It was hypothesized that resistance training by young, healthy, untrained women with protein intakes near recommended levels (0.8 g·kg-1·d-1 would promote bone formation and/or inhibit bone resorption, and that subsequent supplementation to provide 2.4 g protein·kg-1·d-1 would reverse these effects. Methods Bone formation was assessed with serum bone-specific alkaline phosphatase (BAP and osteocalcin (OC, and bone resorption with urinary calcium and deoxypyridinoline (DPD. Biochemical, strength, anthropometric, dietary, and physical activity data were obtained from 24 healthy, untrained, eumenorrheic women (18–29y at baseline, after eight weeks of resistance training (3 d·wk-1, ~1 hr·d-1; 3 sets, 6–10 repetitions, 13 exercises, 75–85% maximum voluntary contraction, and after 12 weeks of resistance training and 10 days of protein/placebo supplementation. Subjects were randomized (double-blind to either a high protein (HP or training control (TC group and, during the final 10 days, consumed either enough purified whey protein to bring daily protein intake to 2.4 g·kg-1·d-1, or an equivalent dose of isoenergetic, carbohydrate placebo. Results Strength, lean tissue mass, and DPD increased significantly in both groups over time, while percent body fat and BAP decreased (repeated measures ANOVA, p ≤ 0.05, Bonferroni correction. No significant changes were observed for serum OC or urinary calcium, and no significant group (TC, HP × time (baseline, week 8, week 12 interactions emerged for any of the biochemical measures. Conclusion (1 Twelve weeks of high-intensity resistance training did not appear to

  10. Growth, intake, and health of Holstein heifer calves fed an enhanced preweaning diet with or without postweaning exogenous estrogen. (United States)

    Geiger, A J; Parsons, C L M; James, R E; Akers, R M


    Research has shown that changes in nutrition both before and after weaning can affect mammary development. Additionally, estrogen is known to be a potent mammogenic stimulant. Our objectives were to determine effects of altered preweaning feeding and exogenous estradiol postweaning on growth, intake, and health. Thirty-six Holstein heifer calves were reared on (1) a restricted milk replacer (MR) diet fed at 0.44kg powder dry matter (DM)/day [R; 20.9% crude protein (CP), 19.8% fat, DM basis], or (2) an enhanced MR fed at 1.08kg powder DM/d (EH; 28.9% CP, 26.2% fat, DM basis). The MR feeding was reduced 50% during wk 8 to prepare for weaning. Starter was offered after wk 4 but balanced between treatments. Body weight and frame were measured weekly with intakes and health monitored daily. At weaning, a subset of calves were slaughtered (n=6/diet). Enhanced-fed calves had greater carcass, thymus, liver, spleen, and mammary gland (parenchyma and mammary fat pad) weights. The EH calves also had greater average daily gain (ADG) starting during wk 1 (0.36 vs. -0.06kg/d) and lasting through wk 7 (1.00 vs. 0.41kg/d). Remaining calves received estrogen implants or placebo and were slaughtered at the end of wk 10, creating 4 treatments: (1) R, (2) R + estrogen (R-E2), (3) EH, and (4) EH + estrogen (EH-E2). Postweaning ADG was similar between R, EH, and EH-E2 calves, but greater in R-E2 calves than E calves. The EH-E2 calves had the heaviest mammary glands, and R-E2 calves had heavier mammary glands than R calves. The EH calves consumed more MR DM, CP, and fat preweaning. The R-fed calves consumed more starter DM preweaning. Fecal score was greater for EH calves (1.74 vs. 1.50) preweaning, but days medicated did not differ. Fecal scores were lower for R-E2 calves postweaning. Improved preweaning feeding of calves increased body weights and frame measures. Differences in body weights remained postweaning. Enhanced-fed calves showed greater ADG during the preweaning period but

  11. The addition of charcoals to broiler diets did not alter the recovery of Salmonella Typhimurium during grow-out. (United States)

    Wilson, K M; Bourassa, D V; Davis, A J; Freeman, M E; Buhr, R J


    Two experiments evaluated prebiotics added to feed on the recovery of Salmonella in broilers during grow-out and processing. In Experiment 1, "seeder" chicks were inoculated with Salmonella Typhimurium and placed with penmates. Treatments were: basal control diet, added 0.3% bamboo charcoal, 0.6% bamboo charcoal, or 0.12% Aromabiotic (medium chain fatty acids). The ceca from seeders and penmates were sampled to confirm Salmonella colonization at 3, 4, and 6 wk, and pen litter was sampled weekly. At 3 wk, charcoal fed chicks had significantly lower cecal recovery (37% lower) of Salmonella via direct plating but no differences at wk 4 or 6. At 6 wk, broilers fed Aromabiotic had no recovery of Salmonella from ceca with direct plating and significantly, 18%, lower recovery with enrichment. In Experiment 2, the treatments were: basal control diet, added 0.3% bamboo charcoal, 0.3% activated bamboo charcoal, or 0.3% pine charcoal. At placement, 2 seeders were challenged with Salmonella and commingled with penmates and ceca sampled at 1 and 2 wk, and ceca from 5 penmates/pen at 3 to 6 wk. Weekly, the pH of the crop and duodenum was measured from 1 penmate/pen and the litter surface sampled. At the end of grow-out broilers were processed. Results showed that penmates had colonized at 1 and 2 wk. Cecal Salmonella showed no differences except at 4 wk, when activated bamboo charcoal had a 18% lower recovery of Salmonella (enrichment) compared to the control (88%). Similar to Experiment 1, the recovery of Salmonella from the litter was not significantly different among treatments, however an overall decrease in recovery by 4 wk with direct plating reoccurred. The pH of the duodenum and the crop were not different among treatments. Crop pH (6.0) for all treatments were significantly higher at wk 1 compared to wk 2 to 6. Charcoals had minimal effect on Salmonella recovery in the ceca, but following defeathering, broilers fed charcoals had significantly lower Salmonella

  12. Muscle Volume Increases Following 16 Weeks of Resistive Exercise Training with the Advanced Resistive Exercise Device (ARED) and Free Weights (United States)

    Nash, R. E.; Loehr, J. A.; Lee, S. M. C.; English, K. L.; Evans, H.; Smith, S. A.; Hagan, R. D.


    Space flight-induced muscle atrophy, particularly in the postural and locomotorymuscles, may impair task performance during long-duration space missions and planetary exploration. High intensity free weight (FW) resistive exercise training has been shown to prevent atrophy during bed rest, a space flight analog. NASA developed the Advanced Resistive Exercise Device (ARED) to simulate the characteristics of FW exercise (i.e. constant mass, inertial force) and to be used as a countermeasure during International Space Station (ISS) missions. PURPOSE: To compare the efficacy of ARED and FW training to induce hypertrophy in specific muscle groups in ambulatory subjects prior to deploying ARED on the ISS. METHODS: Twenty untrained subjects were assigned to either the ARED (8 males, 3 females) or FW (6 males, 3 females) group and participated in a periodizedtraining protocol consisting of squat (SQ), heel raise (HR), and deadlift(DL) exercises 3 d wk-1 for 16 wks. SQ, HR, and DL muscle strength (1RM) was measured before, after 8 wks, and after 16 wks of training to prescribe exercise and measure strength changes. Muscle volume of the vastigroup (V), hamstring group (H), hip adductor group (ADD), medial gastrocnemius(MG), lateral gastrocnemius(LG), and deep posterior muscles including soleus(DP) was measured using MRI pre-and post-training. Consecutive cross-sectional images (8 mm slices with a 2 mm gap) were analyzed and summed. Anatomical references insured that the same muscle sections were analyzed pre-and post-training. Two-way repeated measures ANOVAs (pmuscle strength and volume between training devices. RESULTS: SQ, HR, and DL 1RM increased in both FW (SQ: 49+/-6%, HR: 12+/-2%, DL: 23+/-4%) and ARED (SQ: 31+/-4%, HR: 18+/-2%, DL: 23+/-3%) groups. Both groups increased muscle volume in the V (FW: 13+/-2%, ARED: 10+/-2%), H (FW: 3+/-1%, ARED: 3+/-1 %), ADD (FW: 15=/-2%, ARED: 10+/-1%), LG (FW: 7+/-2%, ARED: 4+/-1%), MG (FW: 7+/-2%, ARED: 5+/-2%), and DP (FW: 2

  13. A randomised placebo-exercise controlled trial of Kung Fu training for improvements in body composition in overweight/obese adolescents: the "Martial Fitness" study. (United States)

    Tsang, Tracey W; Kohn, Michael; Chow, Chin Moi; Singh, M Fiatarone


    The purpose of the study was to investigate if Chinese martial arts (Kung Fu, KF) might be effective for improving body composition, as well as being an appealing form of physical activity for inexperienced, sedentary, overweight/obese adolescents. Twenty subjects (age: 13.3 ± 1.8 y; BMI percentile: 98.6(86.5 - 99.8); 60% girls) were randomly-assigned to the supervised KF or placebo (Tai Chi, TC) control group 3 d.wk(-1) for 6 months. We assessed body composition, including total and regional fat and lean mass, total and regional bone mineral density (BMD), percent lean and fat mass, body mass index and waist circumference, at baseline and after 6 months of training using anthropometry and dual-energy X-ray absorptiometry (DXA). Habitual physical activity and dietary intake were recorded as covariates via self-report at each time-point. As expected due to natural growth, significant increases in height, weight, total and lumbar BMD, and lean mass were seen in the cohort over time, with a trend for increased whole body fat mass, with no difference between groups. By contrast, percent fat and android fat mass via DXA did not increase in either group over time. The absence of a similar expected increase in central adiposity over 6 months could indicate a positive effect of participation in both programs on the metabolically critical abdominal adiposity in this cohort. Further research in this area is warranted to determine ways to increase uptake and compliance, and to see if longer-term martial arts training not only maintains, but improves abdominal fat mass and related metabolic health indices in overweight/ obese adolescents. Key pointsParticipation in our martial arts trial attenuated the increases in body fat mass expected due to growth in our overweight/obese adolescent group.All subjects allocated to the Kung Fu intervention were satisfied with their Kung Fu training, in contrast to our placebo-exercise (Tai Chi) subjects, suggesting that this form of

  14. Effects of supplemental chromium propionate and rumen-protected amino acids on productivity, diet digestibility, and energy balance of peak-lactation dairy cattle. (United States)

    Vargas-Rodriguez, C F; Yuan, K; Titgemeyer, E C; Mamedova, L K; Griswold, K E; Bradford, B J


    Chromium (Cr) feeding in early lactation increased milk production in some studies, but responses to dietary Cr during peak lactation have not been evaluated. Furthermore, interactions of essential amino acids (AA) and Cr have not been explored. Our objective was to evaluate responses to CrPr (KemTRACE chromium propionate 0.04%, Kemin Industries Inc., Des Moines, IA) and rumen-protected Lys (LysiPEARL, Kemin Industries Inc.) and Met (MetiPEARL, Kemin Industries Inc.) and their interaction in peak-lactation cows. Forty-eight individually fed Holstein cows (21 primiparous, 27 multiparous, 38 ± 15 d in milk) were stratified by calving date in 12 blocks and randomly assigned to 1 of 4 treatments within block. Treatments were control, CrPr (8 mg/d of Cr), RPLM (10 g/d of Lys and 5 g/d of Met, intestinally available), or CrPr plus RPLM. Treatments were premixed with ground corn and top-dressed at 200 g/d for 35 d. Diets consisted of corn silage, alfalfa hay, and concentrates, providing approximately 17% crude protein, 31% neutral detergent fiber, and 40% nonfiber carbohydrates. Dry matter intake (DMI) significantly increased with the inclusion of CrPr (22.2 vs. 20.8 ± 0.67 kg/d), and energy-corrected milk (ECM) yield tended to increase. In addition, CrPr increased milk protein yield and tended to increase DMI in primiparous cows but not in multiparous cows. A CrPr×week interaction was detected for milk lactose content, which was increased by CrPr during wk 1 only (4.99 vs. 4.88 ± 0.036%). As a proportion of plasma AA, lysine increased and methionine tended to increase in response to RPLM, but the inclusion of RPLM decreased N efficiency (milk protein N:N intake). Digestible energy intake, gross energy digestibility, and energy balance were not affected by treatments. We observed no treatment effects on feed efficiency or changes in body weight or body condition score. In summary, feeding CrPr increased DMI and tended to increase ECM in cows fed for 5 wk near peak

  15. Physical and mental health-related correlates of physical function in community dwelling older adults: a cross sectional study

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    Burbank Patricia A


    Full Text Available Abstract Background Physical function is the ability to perform both basic and instrumental activities of daily living, and the ability of older adults to reside in the community depends to a large extent on their level of physical function. Multiple physical and health-related variables may differentially affect physical function, but they have not been well characterized. The purpose of this investigation was to identify and examine physical and mental health-related correlates of physical function in a sample of community-dwelling older adults. Methods Nine hundred and four community dwelling older men (n = 263 and women (n = 641 with a mean (95% Confidence Interval age of 76.6 (76.1, 77.1 years underwent tests of physical function (Timed Up and Go; TUG, Body Mass Index (BMI was calculated from measured height and weight, and data were collected on self-reported health quality of life (SF-36, falls during the past 6 months, number of medications per day, depression (Geriatric Depression Scale; GDS, social support, and sociodemographic variables. Results Subjects completed the TUG in 8.7 (8.2, 9.2 seconds and expended 6,976 (6,669, 7,284 Kcal.wk-1 in physical activity. The older persons had a mean BMI of 27. 6 (27.2, 28.0, 62% took 3 or more medications per day, and14.4% had fallen one or more times over the last 6 months. Mean scores on the Mental Component Summary (MCS was 50.6 (50.2, 51,0 and the Physical Component Summary (PCS was 41.3 (40.8, 41.8. Multiple sequential regression analysis showed that, after adjustment for TUG floor surface correlates of physical function included age, sex, education, physical activity (weekly energy expenditure, general health, bodily pain, number of medications taken per day, depression and Body Mass Index. Further, there is a dose response relationship such that greater degree of physical function impairment is associated with poorer scores on physical health-related variables. Conclusions Physical

  16. Grazing dairy cows had decreased interferon-γ, tumor necrosis factor, and interleukin-17, and increased expression of interleukin-10 during the first week after calving. (United States)

    Heiser, Axel; McCarthy, Allison; Wedlock, Neil; Meier, Susanne; Kay, Jane; Walker, Caroline; Crookenden, Mallory A; Mitchell, Murray D; Morgan, Stuart; Watkins, Kate; Loor, Juan J; Roche, John R


    Peripartum, and especially during the transition period, dairy cows undergo dramatic physiological changes. These coincide with an increased risk of disease during the first 2 wk after calving and have been linked to dairy cows failing to achieve production as well as reproductive targets. Previous evidence suggests that these physiological changes affect the immune system and that transition dairy cows experience some form of reduced immunocompetence. However, almost all of these studies were undertaken in high-production, housed dairy cows. Grazing cows have much lower levels of production and this study aimed to provide clarity whether or not the dysfunctional attributes of the peripartum immune system reported in high production housed cows are evident in these animals. Therefore, cell culture techniques, flow cytometry, and quantitative PCR were applied to analyze the cellular composition of peripheral blood mononuclear cells from transition dairy cows as well as the performance of these cells in an in vitro assay. First, a combination of in vitro stimulation and quantitative PCR for cytokines was validated as a quantifiable immunocompetence assay in 29 cattle and a correlation of quantitative PCR and ELISA demonstrated. Second, the relative number of T helper cells, cytotoxic T cells, B cells, γδ T cells, natural killer cells, and monocytes in peripheral blood was measured, of which B cells and natural killer cells increased in number postcalving (n=29) compared with precalving. Third, following in vitro stimulation cytokine profiles indicated decreased expression of IFNγ, tumor necrosis factor, and IL-17 and increased expression of IL-10 wk 1 after calving, which later all returned to precalving values (n=39). Additionally, treatment of transition cows with a nonsteroidal anti-inflammatory drug (i.e., carprofen) administered on d 1, 3, and 5 postcalving (n=19; untreated control n=20) did not affect the cytokine expression at any time point. In conclusion

  17. Production and physiological responses of heat-stressed lactating dairy cattle to conductive cooling. (United States)

    Perano, Kristen M; Usack, Joseph G; Angenent, Largus T; Gebremedhin, Kifle G


    The objective of this research was to test the effectiveness of conductive cooling in alleviating heat stress of lactating dairy cows. A conductive cooling system was built with waterbeds (Dual Chamber Cow Waterbeds, Advanced Comfort Technology Inc., Reedsburg, WI) modified to circulate chilled water. The experiment lasted 7 wk. Eight first-lactation Holstein cows producing 34.4±3.7kg/d of milk at 166±28 d in milk were used in the study. Milk yield, dry matter intake (DMI), and rectal temperature were recorded twice daily, and respiration rate was recorded 5 times per day. During wk 1, the cows were not exposed to experimental heat stress or conductive cooling. For the remaining 6 wk, the cows were exposed to heat stress from 0900 to 1700h each day. During these 6 wk, 4 of the 8 cows were cooled with conductive cooling (experimental cows), and the other 4 were not cooled (control cows). The study consisted of 2 thermal environment exposures (temperature-humidity index mean ± standard deviation of 80.7±0.9 and 79.0±1.0) and 2 cooling water temperatures (circulating water through the water mattresses at temperatures of 4.5°C and 10°C). Thus, a total of 4 conductive cooling treatments were tested, with each treatment lasting 1 wk. During wk 6, the experimental and control cows were switched and the temperature-humidity index of 79.0±1.0 with 4.5°C cooling water treatment was repeated. During wk 7, waterbeds were placed directly on concrete stalls without actively cooling the water. Least squares means and P-values for the different treatments were calculated with multivariate mixed models. Conductively cooling the cows with 4.5°C water decreased rectal temperature by 1.0°C, decreased respiration rate by 18 breaths/min, increased milk yield by 5%, and increased DMI by 14% compared with the controls. When the results from the 2 cooling water temperatures (4.5°C and 10°C circulating water) were compared, we found that the rectal temperature from 4.5

  18. Genetic merit for fertility traits in Holstein cows: IV. Transition period, uterine health, and resumption of cyclicity. (United States)

    Moore, S G; Fair, T; Lonergan, P; Butler, S T


    The objective of this study was to monitor the dry matter intake (DMI), metabolic status, uterine health, and resumption of cyclicity in cows with similar genetic merit for milk production traits but with either good (Fert+) or poor genetic merit (Fert-) for fertility traits. Twenty-six cows were enrolled in the study and data are reported for 15 Fert+ and 10 Fert- cows that completed the study. All cows received a total mixed ration diet during early lactation and were turned out to pasture in late spring. Dry matter intake was recorded daily from wk -2 to 5 relative to parturition. Blood metabolites and metabolic hormones were measured from wk -2 to 8 relative to parturition. Milk production, body condition score, and body weight until wk 35 of lactation are reported. To monitor uterine health, vaginal mucus was scored weekly on a scale of 0 (no pus) to 3 (≥ 50% pus) from parturition to wk 8 and uterine polymorphonuclear neutrophil count was measured at wk 3 and 6 postpartum. Prepartum DMI was similar between genotypes, but Fert+ cows had significantly greater DMI than Fert- cows (19.7 vs. 16.8 kg of dry matter/d) during the postpartum period. Energy balance at wk 1 was significantly greater in Fert+ cows than in Fert- cows [2.3 vs. -1.12 unité fourragère lait (UFL)/d]. The Fert+ cows had significantly greater daily milk solids production (1.89 vs. 1.74 kg/d) and tended to have greater daily milk yield (24.2 vs. 22.3 kg/d). The Fert+ cows had significantly greater mean circulating insulin-like growth factor-I (102.62 vs. 56.85 ng/mL) and tended to have greater mean circulating insulin (3.25 vs. 2.62 μIU/mL) compared with Fert- cows from wk -2 to 8 relative to parturition. Mean circulating glucose (3.40 vs. 3.01 mmol/L) concentrations were significantly greater in Fert+ cows compared with Fert- cows from wk -2 to 3 relative to parturition. The Fert+ cows maintained significantly greater mean body condition score throughout lactation compared with Fert- cows

  19. Response of early-weaned pigs to an enterotoxigenic Escherichia coli (K88) challenge when fed diets containing spray-dried porcine plasma or pea protein isolate plus egg yolk antibody. (United States)

    Owusu-Asiedu, A; Nyachoti, C M; Baidoo, S K; Marquardt, R R; Yang, X


    Enterotoxigenic E. coli (ETEC) infection and resulting scours is a major problem for young pigs, especially when purified plant proteins are fed rather than spray-dried porcine plasma (SDPP). The effect of supplementing a pea protein isolate (PPI)-based diet with egg yolk antibodies (EYA) from laying hens immunized with ETEC K88 antigen on piglet performance, incidence of scours, and gut histology was studied in a 14-d trial. Ninety-six 10-d-old weaned pigs were assigned to five dietary treatments in a completely randomized design to give six replicate pens per treatment. The treatments were PPI without EYA (PPI-EYA), PPI with EYA (PPI+EYA), SDPP without EYA (SDPP-EYA), SDPP with EYA (SDPP+EYA), or a combination of PPI and SDPP (PPI+SDPP). Diets were formulated to similar nutrient levels and provided for ad libitum intake. Blood from all pigs was taken on d 0, 7, and 14 for determining plasma urea N (PUN). On d 7, pigs were orally challenged with 6 mL of 10(10) cfu/ mL ETEC K88. Piglets were weighed on d 7 and 14. On d 7, 8, and 14, four pigs per treatment were sacrificed to study the histology of the small intestine. Weekly feed intake, BW changes, and gain:feed were determined. Fecal swabs from 10 pigs per treatment were taken for a PCR test to detect K88 E. coli. Feed efficiency over the 14-d period was not affected (P > 0.78) by dietary treatment. Mean ADFI on an as-fed basis was lower (P < 0.002) in piglets fed PPI-EYA (64.3 g/d) compared with PPI+EYA (94.8 g/d) or SDPP (102 g/d) during wk 1. Piglets fed PPI-EYA tend to have a lower (P < 0.026) overall ADG (84 g/d) than those fed PPI+EYA (123 g/d) or SDPP (127 g/d) (P < 0.006)-based diets. Although scours was evident in all groups of pigs 6 h after the challenge, most of the piglets fed EYA- or SDPP-containing diets recovered 10 to 72 h postchallenge, whereas those fed PPI-EYA continued to have severe diarrhea, resulting in 33% mortality. The PCR results showed that a greater (P < 0.01) percentage of piglets

  20. Effects of prepartum diets supplemented with rolled oilseeds on calf birth weight, postpartum health, feed intake, milk yield, and reproductive performance of dairy cows. (United States)

    Salehi, R; Colazo, M G; Oba, M; Ambrose, D J


    The objectives were to determine the effects of supplemental fat (no oilseed vs. oilseed) during late gestation and the source of fat (canola vs. sunflower seed), on dry matter intake (DMI), plasma metabolite concentrations, milk production and composition, calf birth weight, postpartum health disorders, ovarian function and reproductive performance in dairy cows. Pregnant Holstein cows, blocked by body condition and parity, were assigned to 1 of 3 diets containing rolled canola seed (high in oleic acid; n=43) or sunflower (high in linoleic acid; n=45) at 8% of dry matter, or no oilseed (control; n=43), for the last 35±2 d of pregnancy. After calving, all cows received a common lactation diet. Blood samples were collected at wk -3 (i.e., 2 wk after initiation of prepartum diets) and at wk +1, +2, +3, +4 and +5 postpartum to determine the concentration of fatty acids (mEq/dL), β-hydroxybutyrate (mg/dL), and glucose (mg/dL). Ovarian ultrasonography was performed twice weekly to determine the first appearance of dominant (10mm) and preovulatory-size (≥16mm) follicles, and ovulation. Uterine inflammatory status based on the proportion of polymorphonuclear leukocytes (PMN; subclinical endometritis: >8% PMN) was assessed at d 25±1 postpartum. Significant parity by treatment interactions were observed for DMI and milk yield. Prepartum oilseed supplementation, more specifically sunflower seed supplementation, increased postpartum DMI in primiparous cows without affecting prepartum DMI or milk yield. Contrarily, in multiparous cows, prepartum oilseed supplementation decreased both prepartum and postpartum DMI and milk yield during the first 2 wk. Regardless of parity, prepartum feeding of canola reduced postpartum DMI compared with those fed sunflower. Mean fatty acids concentrations at wk -3 were greater in cows given supplemental oilseed than those fed no oilseeds. Gestation length and calf birth weight were increased in cows given supplemental oilseed prepartum

  1. Phase I/II study of preoperative chemo-radiotherapy (CT-RT) using twice daily radiation as concomitant boost during two cycles of taxol (T), cisplatin (C), 5-FU (F) in esophageal cancer: normal tissue tolerance and early results

    International Nuclear Information System (INIS)

    Purpose/Objective: Even though preoperative CT has failed to show survival benefit over surgery alone, preoperative CT-RT may provide such survival advantage. The goal of this study was to evaluate an intensified radiotherapy (RT) schedule in preoperative concurrent CT-RT for toxicities, resection rate, tumor downstaging, pathologic complete remission (CR) and treatment outcome. Materials and Methods: Eligibility included biopsy proven squamous or adenocarcinoma, T2-4N0-1M0 lesions, performance status ≤ 2 of ECOG scale, creatinine ≤ 2.0 mg/dl, WBC ≥ 2,500/μl, and platelets ≥ 75,000//μl. CT consisted of cisplatin (P) 20 mg/m2/day (d) x 5 d, 5-FU (F) 800 mg/m2/d continuous infusion x 5 d and Taxol (T) 75-125 mg/m2 (3 hour infusion) on d1 of each cycle. RT delivered 58.5 Gy/34 fractions (F) /5 weeks (wks) to the gross tumor volume by a combination of 45 Gy/25 F/5 wks to a large target volume (6 cm proximal and distal, and 3 cm radial margins beyond the gross tumor) and a boost dose of 13.5 Gy/9 F (1.5 Gy/F x 5 d with the first cycle [wk 1] and 1.5 Gy/F x 4 d with the second cycle [wk 5] of CT) with an interval of ≥ 5 hours between RT to the gross tumor (am) and large target volume (pm) as a means of concomitant boost. Staging work up included barium swallow, chest and head computed tomography, bone scan, esophagoscopy, and endoscopic ultrasound study (EUS). Results: Between April 1995 and February 1997, 38 patients (pts) with locoregional esophageal cancer have been entered into this study. Patient characteristics were as follows: Age 33-84 (median 63), male: female 30 : 8, adenocarcinoma: squamous cell carcinoma 31 : 7. Tumor stages by EUS included T2N0 11 (29%), T2N1 3 (8%), T3N0 14 (37%), T3N1 8 (21%) and T4N0 2 (5%). Taxol dose was escalated from 75 mg/m2 (7 pts) to 125 mg/m2 (5 pts) at which dose limiting toxicities were observed in (3(5)) pts (myocardial infarction, pneumonia, grade 4 neutropenia). The remaining 26 pts have been treated with T 100 mg

  2. Primary study of micropulse diode laser photocoagulation for central serous chorioretinopathy%微脉冲半导体激光治疗CSC的初步观察

    Institute of Scientific and Technical Information of China (English)

    徐建锋; 叶瑞珍; 李贵洲


    AIM: To investigate the effect of micropulse diode laser photocoagulation on central serous choroidretinopathy (CSC). METHODS: Twelve patients diagnosed with CSC by fluorescein angiography ( FFA) and optic coherence tomography ( OCT) examination were treated with micropulse diode laser photocoagulation. The data of visual acuity testing, ophthalmic examination, color photographs, FFA and OCT before photocoagulation and 1 week, 2 weeks, 1 month, 3 months after it were used to evaluate the effects of micropulse diode laser photocoagulation. RESULTS: All patients' self-conscious symptom and visual acuity improved differently. Nine and eleven eyes leakage point disappeared after micropulse diode laser photocoagulation in 2 weeks and one month in FFA. Nine and eleven eyes of subretinal effusion in OCT were absorbed after laser photocoagulation in 2 weeks and one month. All cases' leakage point and subretinal effusion disappeared after 3 months. The window defect in FFA did not occur in all laser treated eyes. CONCLUSION: The micropulse diode laser photocoagulation could shorten the course of CSC, improve the patient's visual acuity and reduce the complications.%目的:探讨微脉冲半导体激光治疗中心性浆液性脉络膜视网膜病变(central serous chorioretinopathy,CSC)的疗效.方法:自身对照病例研究.将经荧光素眼底血管造影(fundus fluorescein angiography,FFA)和光学相干断层扫描(optic coherence tomography,OCT)检查确诊的典型性CSC患者12例12眼行微脉冲半导体激光治疗前及治疗后随访观察1,2wk;1,3mo的临床资料进行分析,主要以治疗前后的视力、自觉症状、眼底情况、FFA及OCT检查的改变为观察指标,以评价微脉冲半导体激光对CSC的治疗效果.结果:所有患者自觉症状减轻或消退,视力及视觉质量均有不同程度改善.激光光凝2wk和1mo后,分别有9眼(75.0%)和11眼(91.7%)的黄斑区水肿完全消退,3mo后全部患者黄斑区水肿消退.光凝2

  3. 16 Weeks of Training with the International Space Station Advanced Resistive Exercise Device (aRED) Is not Different than Training with Free Weights (United States)

    Loehr, J. A.; Lee, S. M. C.; English, K. E.; Leach, M.; Bentley, J.; Nash, R.; Hagan, R. D.


    The advanced Resistive Exercise Device (aRED) is a resistive exercise system designed to maintain muscle mass and strength in microgravity by simulating free weight (FW) exercise. aRED utilizes vacuum cylinders and inertial flywheels to replicate the constant mass and inertial components, respectively, of FW exercise in normal gravity. PURPOSE: To compare the effectiveness of aRED and FW resistive exercise training in ambulatory subjects. METHODS: Untrained subjects were assigned to two groups, FW (6 males, 3 females) and aRED (8 males, 3 females), and performed squat (SQ), heel raise (HR), and deadlift (DL) exercises 3 d wk-1 for 16 wks. SQ, HR and DL strength (1RM) were measured using FW hardware pre-, mid- and post-training. Subjects participated in a periodized training protocol with the exercise prescription based on a percentage of 1RM. Thigh and lower leg muscle volume were assessed using Magnetic Resonance Imaging (MRI), and leg (LLM) and total body lean mass (BLM) were measured using Dual Energy X-ray Absorptiometry (DXA) pre- and post-training. RESULTS: SQ 1RM increased in both FW (48.9+/-6.1%) and aRED (31.2+/-3.8%) groups, and there was a greater training response in FW compared with aRED (p=0.01). HR and DL 1RM increased in FW (HR: 12.3+/-2.4%, DL: 23.3+/-4.4%) and aRED (HR: 18.0+/-1.6%, DL: 23.2+'-2.8%), but there were no differences between groups. Thigh muscle volume was greater following training in both groups (FW: 9.8+/-0.9%, aRED: 7.1+/-1.2%) but lower leg muscle volume increased only in the FW group (3.0+/-1.1%). Lean tissue mass increased in both FW (LLM: 3.9+/-1.1%, BLM: 2.5+/-0.7%) and aRED (LLM: 4.8+/-0.7%, BLM: 2.6 0.7%). There were no between group differences in muscle volume or lean mass in response to training. CONCLUSIONS: In general, the increase in muscle strength, muscle volume, and lean tissue mass when training with aRED was not different than when using the same training protocol with FW. The smaller increase in SQ 1RM in the a

  4. Pulmonary toxicity screening studies in male rats with M5 respirable fibers and particulates. (United States)

    Warheit, David B; Webb, Thomas R; Reed, Kenneth L


    M5 fiber is a high-strength, high-performance organic fiber type that is a rigid rod material and composed of heterocyclic polymer fibers of type PIPD. The aim of this study was to evaluate the acute lung toxicity of intratracheally instilled M5 respirable fibers and particulates in rats. Using a pulmonary bioassay and bridging methodology, the acute lung toxicity of intratracheally instilled M5 particulates and that of its fibers were compared with a positive control particle type, quartz, as well as a negative control particle type, carbonyl iron particles. Moreover, the results of these instillation studies were bridged with data previously generated from inhalation studies with quartz and carbonyl iron particles, using the quartz and iron particles as the inhalation/instillation bridge material. For the bioassay experimental design, in the bronchoalveolar lavage studies, the lungs of rats were intratracheally instilled with 0.5 or 0.75 mg/kg of M5 particulate or 1 or 5 mg/kg of the following control or particle types: (1) M5 long fiber preparation, (2) silica-quartz particles, and (3) carbonyl iron particles. Phosphate-buffered saline (PBS)-instilled rats served as additional controls. Following exposures, the lungs of PBS and particle-exposed rats were assessed using bronchoalveolar lavage (BAL) fluid biomarkers, cell proliferation methods, and histopathological evaluation of lung tissue at 24 h, 1 wk, 1 mo and 3 mo post instillation exposure. The bronchoalveolar lavage results demonstrated that lung exposures to quartz particles, at both concentrations but particularly at the higher dose, produced significant increases vs. controls in pulmonary inflammation and cytotoxicity indices. Exposures to M5 particulate and M5 long fiber preparation produced transient inflammatory and cell injury effects at 24 h postexposure (pe) as well as at 24 h and 1 wk pe, respectively, but these effects were not sustained when compared to quartz-silica effects. Exposures to

  5. Energy dissipation in biomolecular machines

    Energy Technology Data Exchange (ETDEWEB)

    Lervik, Anders


    The operation of a molecular pump, the calcium pump of sarcoplasmic reticulum is studied using mesoscopic non-equilibrium thermodynamics and molecular dynamics. The mesoscopic non-equilibrium thermodynamic description of the pump is compared to the description obtained in the framework of Hill for kinetic enzyme cycles. By comparing these two descriptions at isothermal conditions, they are found to be equivalent. This supports the validity of the mesoscopic approach. An extension of the mesoscopic non-equilibrium framework to also include a heat flux and the corresponding temperature difference is proposed. This can be used to model phenomena such as non-shivering thermogenesis, a process which lack a theoretical description in the kinetic cycle picture. Further, the heat transfer in the calcium pump is studied using molecular dynamics. This is done in order to obtain phenomenological parameters that can be used for the modeling of thermogenesis. A non-stationary non-equilibrium molecular dynamics approach is developed, which may be used to study heat transfer between a small object and the surrounding solvent. This methodology is applied to the calcium pump solvated in water. It is found that the thermal conductivity of the protein is low (0.2 W K-1 m-1) compared to water (0.6 WK-1 m-1). This means that the protein may sustain a large temperature gradient across its structure. The simulations also show that the protein-water surface is important for the heat transfer. The time scale for vibrational energy relaxation is found to be of order 10/100 ps which strengthens the local equilibrium assumption of mesoscopic non-equilibrium thermodynamics. Mesoscopic non-equilibrium thermodynamics is also applied to calculate the thermodynamic efficiency of the calcium pump embedded in lipid bilayers of varying length and from different tissues. This is done in order to show the applicability of mesoscopic non-equilibrium thermodynamics to interpret experimental data. The

  6. Laparoscopic cholelithotomy and polypectomy of gallbladder polyps for cholelithiasis and/or cholecystic polyps: An analysis of 53 cases%腹腔镜保胆取石术和息肉切除术临床分析53例

    Institute of Scientific and Technical Information of China (English)

    徐新保; 张辉; 张洪义; 刘承利; 何晓军; 肖梅; 张宏义; 冯志强


    例胆固醇性息肉行胆囊底部切开取息肉术,3例腺瘤性息肉患者行息肉周围胆囊部分切除术;6例胆囊结石合并息肉的患者均行胆囊底部切开取石(息肉)术,其息肉均为胆固醇性息肉.胆囊切口采用4-0 prolene 缝线连续缝合10例,采用3-0可吸收线间断缝合或连续缝合43例.术中使用胆道镜33例,20例未用胆道镜,用腹腔镜代替胆道镜查看胆囊内结石或息肉是否取净.术后放置腹腔引流管19例,其余均未放置腹腔引流管.胆囊结石(包括合并胆囊息肉)患者术后服用利胆药物者33例,服药时间为1 wk-1 mo不等,另12例未服利胆药物.结果:53例保胆患者手术均获成功,没有1例中转剖腹手术.手术时间80-200 min,平均109 min±33 min.1例颈部切开取石患者术后出现上腹疼痛,腹腔引流管每日引出约15 mL淡黄色腹水,超声检查显示小网膜腔少量积液,考虑少量胆漏所致,术后5d腹痛缓解,腹腔引流管无液体引出,拔除腹腔引流管后无不适.其余患者术后均恢复顺利,术后住院2-10d,平均3.8 d±2.2 d,均治愈出院.所有患者均获随访,随访时间3-36 mo,1例患者术后12 mo胆囊泥沙样结石复发,给予利胆排石药物治疗1 mo后复查腹部超声显示胆囊结石消失.结论:腹腔镜下保胆取石和取息肉术保留了胆囊及胆囊功能,有利于维护患者消化系统的正常功能.只要胆囊大小基本正常、胆囊壁增厚不明显、胆囊功能基本正常,排除了胆囊恶性病变,并且患者及家属保胆愿望强烈,均可进行腹腔镜保胆取石(息肉)术.