WorldWideScience

Sample records for annual publication ngs

  1. 77 FR 26585 - Annual Public Meeting

    Science.gov (United States)

    2012-05-04

    ... person and submit a brief statement of the general nature of the comments they wish to present. Written... THE FOOD AND DRUG ADMINISTRATION Annual Public Meeting ACTION: Notice of annual meeting. SUMMARY: The... Food and Drug Amendments of 2007, is announcing an annual open public meeting. The Foundation...

  2. 78 FR 23960 - Annual Public Meeting

    Science.gov (United States)

    2013-04-23

    ... person and submit a brief statement of the general nature of the comments they wish to present. Written... THE FOOD AND DRUG ADMINISTRATION Annual Public Meeting ACTION: Notice of annual meeting. SUMMARY: The... Food and Drug Amendments of 2007, is announcing an annual open public meeting. The Foundation...

  3. A framework for organizing cancer-related variations from existing databases, publications and NGS data using a High-performance Integrated Virtual Environment (HIVE).

    Science.gov (United States)

    Wu, Tsung-Jung; Shamsaddini, Amirhossein; Pan, Yang; Smith, Krista; Crichton, Daniel J; Simonyan, Vahan; Mazumder, Raja

    2014-01-01

    Years of sequence feature curation by UniProtKB/Swiss-Prot, PIR-PSD, NCBI-CDD, RefSeq and other database biocurators has led to a rich repository of information on functional sites of genes and proteins. This information along with variation-related annotation can be used to scan human short sequence reads from next-generation sequencing (NGS) pipelines for presence of non-synonymous single-nucleotide variations (nsSNVs) that affect functional sites. This and similar workflows are becoming more important because thousands of NGS data sets are being made available through projects such as The Cancer Genome Atlas (TCGA), and researchers want to evaluate their biomarkers in genomic data. BioMuta, an integrated sequence feature database, provides a framework for automated and manual curation and integration of cancer-related sequence features so that they can be used in NGS analysis pipelines. Sequence feature information in BioMuta is collected from the Catalogue of Somatic Mutations in Cancer (COSMIC), ClinVar, UniProtKB and through biocuration of information available from publications. Additionally, nsSNVs identified through automated analysis of NGS data from TCGA are also included in the database. Because of the petabytes of data and information present in NGS primary repositories, a platform HIVE (High-performance Integrated Virtual Environment) for storing, analyzing, computing and curating NGS data and associated metadata has been developed. Using HIVE, 31 979 nsSNVs were identified in TCGA-derived NGS data from breast cancer patients. All variations identified through this process are stored in a Curated Short Read archive, and the nsSNVs from the tumor samples are included in BioMuta. Currently, BioMuta has 26 cancer types with 13 896 small-scale and 308 986 large-scale study-derived variations. Integration of variation data allows identifications of novel or common nsSNVs that can be prioritized in validation studies. Database URL: BioMuta: http

  4. NgsRelate

    DEFF Research Database (Denmark)

    Korneliussen, Thorfinn Sand; Moltke, Ida

    2015-01-01

    MOTIVATION: Pairwise relatedness estimation is important in many contexts such as disease mapping and population genetics. However, all existing estimation methods are based on called genotypes, which is not ideal for next-generation sequencing (NGS) data of low depth from which genotypes cannot...... be called with high certainty. RESULTS: We present a software tool, NgsRelate, for estimating pairwise relatedness from NGS data. It provides maximum likelihood estimates that are based on genotype likelihoods instead of genotypes and thereby takes the inherent uncertainty of the genotypes into account....... Using both simulated and real data, we show that NgsRelate provides markedly better estimates for low-depth NGS data than two state-of-the-art genotype-based methods. AVAILABILITY: NgsRelate is implemented in C++ and is available under the GNU license at www.pop gen.dk/software. CONTACT: ida...

  5. Director of Public Health annual report 2009

    OpenAIRE

    Public Health Agency

    2010-01-01

    This first annual report of the Director of Public Health highlights the many public health challenges that affect people in Northern Ireland and how thepublic health team tackles this complex agenda by working with many statutory, community and voluntary partner organisations across health, local government, education, housing and other sectors. The report refers to core tables throughout, these tables provide key statistical data on population, birth and death rates, mortality by cause, l...

  6. NGS Absolute Gravity Data

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The NGS Absolute Gravity data (78 stations) was received in July 1993. Principal gravity parameters include Gravity Value, Uncertainty, and Vertical Gradient. The...

  7. NGS Survey Control Map

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The NGS Survey Control Map provides a map of the US which allows you to find and display geodetic survey control points stored in the database of the National...

  8. Pickering NGS decontaminations

    International Nuclear Information System (INIS)

    In early 1984, decontaminations of the Pickering NGS Units 1 and 2 heat transport systems were carried out. These decontaminations reduced radiation fields in front of the reactor face by up to a factor of 10, and resulted in radiation fields of 50 to 140 mR/h. These decontaminations were carried out using an improved version of the CAN-DECON process. This paper describes the development of the process and its successful applications at Pickering NGS

  9. 75 FR 39034 - Public Housing Annual Contributions Contract

    Science.gov (United States)

    2010-07-07

    ... URBAN DEVELOPMENT Public Housing Annual Contributions Contract AGENCY: Office of the Chief Information... conditions contained in an Annual Contributions Contract (ACC) with certain requirements applicable to all... electronic submission of responses. This notice also lists the following information: Title of...

  10. 77 FR 43618 - Sunshine Act Meetings; OPIC Annual Public Hearing

    Science.gov (United States)

    2012-07-25

    ... CORPORATION Sunshine Act Meetings; OPIC Annual Public Hearing TIME AND DATE: 2:00 p.m., Thursday, September 6..., DC. STATUS: Hearing OPEN to the Public at 2:00 p.m. PURPOSE: Annual Public Hearing to afford an... INFORMATION: OPIC is a U.S. Government agency that provides, on a commercial basis, political risk...

  11. 75 FR 76758 - Sunshine Act; OPIC Annual Public Hearing

    Science.gov (United States)

    2010-12-09

    ... CORPORATION Sunshine Act; OPIC Annual Public Hearing Time and Date: 3:30 p.m., Thursday, January 20, 2011.... Status: Hearing OPEN to the Public at 3:30 p.m. Purpose: Annual Public Hearing to afford an opportunity...: OIC is a U.S. Government agency that provides, on a commercial basis, political risk insurance...

  12. 78 FR 5516 - Sunshine Act: OPIC Annual Public Hearing

    Science.gov (United States)

    2013-01-25

    ... CORPORATION Sunshine Act: OPIC Annual Public Hearing TIME AND DATE: 2 p.m., Wednesday, March 13, 2013. PLACE...: Hearing open to the public at 2 p.m. PURPOSE: Annual Public Hearing to afford an opportunity for any... INFORMATION: OPIC is a U.S. Government agency that provides, on a commercial basis, political risk...

  13. NGS catalog: A database of next generation sequencing studies in humans.

    Science.gov (United States)

    Xia, Junfeng; Wang, Qingguo; Jia, Peilin; Wang, Bing; Pao, William; Zhao, Zhongming

    2012-06-01

    Next generation sequencing (NGS) technologies have been rapidly applied in biomedical and biological research since its advent only a few years ago, and they are expected to advance at an unprecedented pace in the following years. To provide the research community with a comprehensive NGS resource, we have developed the database Next Generation Sequencing Catalog (NGS Catalog, http://bioinfo.mc.vanderbilt.edu/NGS/index.html), a continually updated database that collects, curates and manages available human NGS data obtained from published literature. NGS Catalog deposits publication information of NGS studies and their mutation characteristics (SNVs, small insertions/deletions, copy number variations, and structural variants), as well as mutated genes and gene fusions detected by NGS. Other functions include user data upload, NGS general analysis pipelines, and NGS software. NGS Catalog is particularly useful for investigators who are new to NGS but would like to take advantage of these powerful technologies for their own research. Finally, based on the data deposited in NGS Catalog, we summarized features and findings from whole exome sequencing, whole genome sequencing, and transcriptome sequencing studies for human diseases or traits.

  14. Embalse NGS PLEX overview

    International Nuclear Information System (INIS)

    Embalse Nuclear Generating Station (ENGS), a CANDU 6 type with 648 MWe output, started operation in 1983. In the last 10 years it has shown an excellent performance with an average capacity factor of 88.25 %. An effective plant operation and management of the effects of aging on equipment, piping, accessories, components and structures that constitute plant principal systems by means of a continuos inspection program; is required. The end of the design life is foreseen for 2011. However, a political decision about the intention for an Embalse PLEX has been taken. In this paper an analysis of the Embalse-NGS Program for a possible Plant Life Extension (PLEx) is presented. A typical program for a CANDU PLEX has three main phases: Phase 1: Pre-project definition. Phase 2: Project Engineering and preparation. Phase 3: Project Implementation. However, there are also other programs, for example, In Service Inspection (ISI), Preventive / Predictive Maintenance (call-up cards), Routine Testing, Operating Routine, which focus on maintaining a high reliability of safety and process systems, as well. These are not included herein but their contributions to the Aging Assessment (AA) as well as to the performance of the plant are very important. (author)

  15. Airborne Gravity Data Enhances NGS Experimental Gravimetric Geoid in Alaska

    Science.gov (United States)

    Holmes, S. A.; Childers, V. A.; Li, X.; Roman, D. R.

    2014-12-01

    The U.S. National Geodetic Survey [NGS], through their Gravity for the Redefinition of the American Vertical Datum [GRAV-D] program, continues to update its gravimetry holdings by flying new airborne gravity surveys over a large fraction of the USA and its territories. By 2022, NGS intends that all orthometric heights in the USA will be determined in the field by using a reliable national gravimetric geoid model to transform from geodetic heights obtained from GPS. Several airborne campaigns have already been flown over Alaska and its coastline. Some of this Alaskan coastal data have been incorporated into a new NGS experimental geoid model - xGEOID14. The xGEOID14 model is the first in a series of annual experimental geoid models that will incorporate NGS GRAV-D airborne data. This series provides a useful benchmark for assessing and improving current techniques by which the airborne and land-survey data are filtered and cleaned, and then combined with satellite gravity models, elevation data (etc.) with the ultimate aim of computing a geoid model that can support a national physical height system by 2022. Here we will examine the NGS GRAV-D airborne data in Alaska, and assess its contribution to xGEOID14. Future prospects for xGEOID15 will also be considered.

  16. The 41st Annual Phi Delta Kappa/Gallup Poll of the Public's Attitudes toward the Public Schools

    Science.gov (United States)

    Bushaw, William J.; McNee, John A.

    2009-01-01

    The authors report the findings of the 41st Annual Phi Delta Kappa/Gallup Poll of the Public's Attitudes toward the Public Schools. The annual PDK/Gallup Poll of the Public's Attitudes toward the Public Schools is unique because it's not an advocacy poll, but rather a thoughtful study of Americans' current perceptions of their public schools. Each…

  17. NGS-based deep bisulfite sequencing.

    Science.gov (United States)

    Lee, Suman; Kim, Joomyeong

    2016-01-01

    We have developed an NGS-based deep bisulfite sequencing protocol for the DNA methylation analysis of genomes. This approach allows the rapid and efficient construction of NGS-ready libraries with a large number of PCR products that have been individually amplified from bisulfite-converted DNA. This approach also employs a bioinformatics strategy to sort the raw sequence reads generated from NGS platforms and subsequently to derive DNA methylation levels for individual loci. The results demonstrated that this NGS-based deep bisulfite sequencing approach provide not only DNA methylation levels but also informative DNA methylation patterns that have not been seen through other existing methods.•This protocol provides an efficient method generating NGS-ready libraries from individually amplified PCR products.•This protocol provides a bioinformatics strategy sorting NGS-derived raw sequence reads.•This protocol provides deep bisulfite sequencing results that can measure DNA methylation levels and patterns of individual loci.

  18. The 33rd Annual Phi Delta Kappa/Gallup Poll of the Public's Attitudes Toward the Public Schools.

    Science.gov (United States)

    Rose, Lowell C.; Gallup, Alec M.

    2001-01-01

    Presents results of the 33rd Annual Phi Delta Kappa/Gallup Poll on the public's attitudes toward the public schools. Includes a summary of key findings and tables showing percentage distributions of responses to each question. (PKP)

  19. Toward Better Oversight of NGS Tests.

    Science.gov (United States)

    2016-09-01

    The FDA has published two draft guidance documents aimed at streamlining its oversight of tests based on next-generation sequencing (NGS). One contains preliminary recommendations addressing the analytic validity of NGS-based tests for hereditary diseases; the other explains how test developers can obtain official recognition of their genetic variant databases, potentially speeding marketing clearance or approval.

  20. Toward Better Oversight of NGS Tests.

    Science.gov (United States)

    2016-09-01

    The FDA has published two draft guidance documents aimed at streamlining its oversight of tests based on next-generation sequencing (NGS). One contains preliminary recommendations addressing the analytic validity of NGS-based tests for hereditary diseases; the other explains how test developers can obtain official recognition of their genetic variant databases, potentially speeding marketing clearance or approval. PMID:27457361

  1. San Francisco Bay National Wildlife Refuge : Annual Narrative 1991 : Public use sections

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This document contains the public use sections of the 1991 Annual Narrative Report for Don Edwards San Francisco Bay National Wildlife Refuge. Public use activities...

  2. Public use at San Francisco Bay National Wildlife Refuge : Annual Narrative 1993

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This document contains the public use sections of the 1993 Annual Narrative Report for Don Edwards San Francisco Bay National Wildlife Refuge. Public use activities...

  3. Public use at San Francisco Bay National Wildlife Refuge : Annual Narrative 1994

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This document contains the public use sections of the 1994 Annual Narrative Report for Don Edwards San Francisco Bay National Wildlife Refuge. Public use activities...

  4. The 38th Annual Phi Delta Kappa/Gallup Poll of the Public's Attitudes toward the Public Schools

    Science.gov (United States)

    Rose, Lowell C.; Gallup, Alec M.

    2006-01-01

    Rose and Gallup report on the results of the 38th Annual PDK/Gallup Poll of the Public's Attitude Toward the Public Schools. This year's survey examined No Child Left Behind and the public's perception of the law, the appropriate role of standardized testing, the achievement gap between white students and black and Hispanic students, the…

  5. The 39th Annual Phi Delta Kappa/Gallup Poll of the Public's Attitudes toward the Public Schools

    Science.gov (United States)

    Rose, Lowell C.; Gallup, Alec M.

    2007-01-01

    In this article, the authors report the results of the 39th Annual Phi Delta Kappa/Gallup Poll of the public's attitudes toward the public schools. This year's report examined the public's assessment of the No Child Left Behind (NCLB) and its principal strategy, standardized testing. The authors include a summary of key findings and tables showing…

  6. TOGGLE: toolbox for generic NGS analyses

    OpenAIRE

    Monat, Cécile; Tranchant-Dubreuil, Christine; Kougbeadjo, Ayité; Farcy , Cédric; Ortega-Abboud, Enrique; Amanzougarene, Souhila; Ravel, Sébastien; Agbessi, Mawusse; Orjuela-Bouniol, Julie; Summo, Marilyne; Sabot, François

    2015-01-01

    Background: The explosion of NGS (Next Generation Sequencing) sequence data requires a huge effort in Bioinformatics methods and analyses. The creation of dedicated, robust and reliable pipelines able to handle dozens of samples from raw FASTQ data to relevant biological data is a time-consuming task in all projects relying on NGS. To address this, we created a generic and modular toolbox for developing such pipelines. Results: TOGGLE (TOolbox for Generic nGs anaLysEs) is a suite of tools abl...

  7. TOGGLE: toolbox for generic NGS analyses

    OpenAIRE

    Monat, Cécile; Tranchant-Dubreuil, Christine; Kougbeadjo, Ayité; Farcy, Cédric; Ortega-Abboud, Enrique; Amanzougarene, Souhila; Ravel, Sébastien; Agbessi, Mawusse; Orjuela-Bouniol , Julie; Summo, Marilyne; Sabot, François

    2015-01-01

    Background The explosion of NGS (Next Generation Sequencing) sequence data requires a huge effort in Bioinformatics methods and analyses. The creation of dedicated, robust and reliable pipelines able to handle dozens of samples from raw FASTQ data to relevant biological data is a time-consuming task in all projects relying on NGS. To address this, we created a generic and modular toolbox for developing such pipelines. Results TOGGLE (TOolbox for Generic nGs anaLysEs) is a suite of tools able ...

  8. The ICR142 NGS validation series: a resource for orthogonal assessment of NGS analysis

    OpenAIRE

    Elise Ruark; Anthony Renwick; Matthew Clarke; Katie Snape; Emma Ramsay; Anna Elliott; Sandra Hanks; Ann Strydom; Sheila Seal; Nazneen Rahman

    2016-01-01

    To provide a useful community resource for orthogonal assessment of NGS analysis software, we present the ICR142 NGS validation series. The dataset includes high-quality exome sequence data from 142 samples together with Sanger sequence data at 730 sites; 409 sites with variants and 321 sites at which variants were called by an NGS analysis tool, but no variant is present in the corresponding Sanger sequence. The dataset includes 286 indel variants and 275 negative indel sites, and thus the I...

  9. GATB: Toolbox for developing efficient NGS software

    OpenAIRE

    Drezen, E; Drezen, Erwan; Rizk, G; Chikhi, R; Deltel, Charles; Lemaitre, C.; Peterlongo, P.; Lavenier, D

    2014-01-01

    International audience The analysis of NGS data remains a time and space-consuming task. Many efforts have been made to provide efficient data structures for indexing the terabytes of data generated by the fast sequencing machines (Suffix Array, Burrows-Wheeler transform, Bloom Filter, etc.). Mapper tools, genome assemblers, SNP callers, etc., make an intensive use of these data structures to keep their memory footprint as lower as possible. The overall efficiency of NGS software is brough...

  10. Improved Error Correction of NGS Data

    OpenAIRE

    Alic, Andrei Stefan

    2016-01-01

    [EN] The work done for this doctorate thesis focuses on error correction of Next Generation Sequencing (NGS) data in the context of High Performance Computing (HPC). Due to the reduction in sequencing cost, the increasing output of the sequencers and the advancements in the biological and medical sciences, the amount of NGS data has increased tremendously. Humans alone are not able to keep pace with this explosion of information, therefore computers must assist them to ease the handle...

  11. The 34th Annual Phi Delta Kappa/Gallup Poll of the Public's Attitudes toward the Public Schools.

    Science.gov (United States)

    Rose, Lowell C.; Gallup, Alec M.

    2002-01-01

    Results of the 34th Annual Phi Delta Kappa/Gallup Poll of the public's attitudes toward the public schools. Reports, for example, that school-age parents continue to regard local schools favorably, 71 percent of whom give the school attended by their oldest child a grade of A or B. (PKP)

  12. Director of Public Health annual report 2009 and core tables 2008

    OpenAIRE

    Public Health Agency

    2010-01-01

    This first annual report of the Director of Public Health highlights the many public health challenges that affect people in Northern Ireland and how the�public health team tackles this complex agenda by working with many statutory, community and voluntary partner organisations across health, local government, education, housing and other sectors.

  13. Director of Public Health annual report 2010 and core tables 2009

    OpenAIRE

    Public Health Agency

    2011-01-01

    This�second annual report of the Director of Public Health highlights the many public health challenges that affect people in Northern Ireland.It demonstrates how the public health team tackles this complex agenda by working with many statutory, community and voluntary partner organisations across health, local government, education, housing and other sectors.

  14. Estimating inbreeding coefficients from NGS data

    DEFF Research Database (Denmark)

    Vieira, Filipe Garrett; Fumagalli, Matteo; Albrechtsen, Anders;

    2013-01-01

    Most methods for Next-Generation Sequencing (NGS) data analyses incorporate information regarding allele frequencies using the assumption of Hardy-Weinberg Equilibrium (HWE) as a prior. However, many organisms including domesticated, partially selfing or with asexual life cycles show strong...... deviations from HWE. For such species, and specially for low coverage data, it is necessary to obtain estimates of inbreeding coefficients (F) for each individual beforecalling genotypes. Here, we present two methods for estimating inbreeding coefficients from NGS data based on an Expectation...

  15. 78 FR 11915 - Order Approving Public Company Accounting Oversight Board Budget and Annual Accounting Support...

    Science.gov (United States)

    2013-02-20

    ..., Release No. 68921/February 13, 2013] Order Approving Public Company Accounting Oversight Board Budget and Annual Accounting Support Fee for Calendar Year 2013 The Sarbanes-Oxley Act of 2002, as amended (the ``Sarbanes-Oxley Act''),\\1\\ established the Public Company Accounting Oversight Board (``PCAOB'') to...

  16. 77 FR 2576 - Order Approving Public Company Accounting Oversight Board Budget and Annual Accounting Support...

    Science.gov (United States)

    2012-01-18

    ... Release No. 34-66141/January 11, 2012] Order Approving Public Company Accounting Oversight Board Budget and Annual Accounting Support Fee for Calendar Year 2012 The Sarbanes-Oxley Act of 2002, as amended (the ``Sarbanes-Oxley Act''),\\1\\ established the Public Company Accounting Oversight Board...

  17. Statistical modelling of spatio-temporal dependencies in NGS data

    NARCIS (Netherlands)

    Ranciati, Saverio

    2016-01-01

    Next-generation sequencing (NGS) heeft zich snel gevestigd als de huidige standaard in de genetische analyse. Deze omschakeling van microarray naar NGS vereist nieuwe statistische strategieën om de onderzoeksvragen aan te pakken. Ten eerste, NGS data bestaat uit discrete waarnemingen, meestal gekenm

  18. Changing Expectations of College: The 47th Annual PDK/Gallup Poll of the Public's Attitudes toward the Public Schools

    Science.gov (United States)

    Phi Delta Kappan, 2015

    2015-01-01

    This installment reporting on the 47th Annual PDK/Gallup Poll of American's Attitudes Toward the Public Schools focuses on American's views of the value of a high school and a college education and the affordability of college. It is the first year the poll included enough respondents to be able to break out sentiments of specific demographic…

  19. Diagnostic NGS for Severe Neuromuscular Disorders

    OpenAIRE

    Radhika Dhamija; Chelsea Chambers

    2015-01-01

    Investigators from the University of Western Australia report the diagnostic yield of performing next generation sequencing (NGS; whole exome and targeted capture of 277 neuromuscular genes) in a heterogenous cohort of patients with neuromuscular disorders (NMD) presenting at or before birth.

  20. The potential of whole genome NGS for infectious disease diagnosis.

    Science.gov (United States)

    Lecuit, Marc; Eloit, Marc

    2015-01-01

    Non-targeted identification of microbes is now possible directly in biological samples, based on whole-genome-NGS (WG-NGS) techniques that allow deep sequencing of nucleic acids, data mining and sorting out of sequences of pathogens without any a priori hypothesis. WG-NGS was first only used as a research tool due to its cost, complexity and lack of standardization. Recent improvements in sample preparation and bioinformatics pipelines and decrease in cost now allow actionable diagnostics in patients. The potency and limits of WG-NGS and possible future indications are discussed here. WG-NGS will likely soon become a standard procedure in microbiological diagnosis.

  1. Public Health Agency Annual Business Plan 2015-2016

    OpenAIRE

    Public Health Agency

    2015-01-01

    This Business Plan sets out the key priorities and actions that will be progressed by the Public Health Agency (PHA) in 2015/16. The PHA believes that these actions will have the biggest impact on improving levels of health and social wellbeing, protecting the health of the community, and ensuring patients continue to receive high quality and safe treatment and care services.The business plan is available to download below.

  2. The CERN Neutrino beam to Gran Sasso (NGS)

    CERN Document Server

    Bailey, R; Ball, A E; Bonnal, P; Buhler-Broglin, Manfred; Détraz, C; Elsener, Konrad; Ereditato, A; Faugeras, Paul E; Ferrari, A; Fortuna, G; Grant, A L; Guglielmi, A M; Hilaire, A; Hübner, Kurt; Jonker, M; Kissler, Karl Heinz; López-Hernandez, L A; Maugain, J M; Migliozzi, P; Palladino, Vittorio; Pietropaolo, F; Revol, Jean Pierre Charles; Sala, P R; Sanelli, C; Stevenson, Graham Roger; Vassilopoulos, N; Vincke, H H; Weisse, E; Wilhemsson, M

    1999-01-01

    The conceptual technical design of the NGS (CERN neutrino beam to Gran Sasso) facility has been presented in the report CERN 98-02 / INFN-AE/98-05. Additional information, in particular an update on various neutrino beam options for the NGS facility, has been provided in a memorandum to the CERN-SPSC Committee (CERN-SPSC/98-35). In the present report, further improvements on the NGS design and performance, in particular new scenarios for SPS proton cycles for NGS operation and a new version of the NGS "high energy" neutrino beam for nt appearance experiments, are described. This new NGS reference beam is estimated to provide three times more nt events per year than the beam presented in the 1998 report. The radiological aspects of the NGS facility have been re-examined with the new beam design. An updated version of the construction schedule is also presented.

  3. Assessment of ichthyoplankton entrainment at Pickering 'A' NGS using a pump/net in lake system

    International Nuclear Information System (INIS)

    Annual entrainment at Pickering 'A' NGS was estimated for alewife as 13.6 X 106 larvae and 409 X 106 eggs. A substantial portion of eggs and larvae entering the intake were dead due to natural mortality (41%-81%) prior to entrainment. Viable eggs and larvae, immediately following entrainment showed mortalities of 54% and 44% respectively. The latent mortality of entrained eggs was 100% (48 h)

  4. 5 CFR 2638.704 - Annual ethics training for public filers.

    Science.gov (United States)

    2010-01-01

    ... year, agencies must give verbal ethics training to employees who are required by 5 CFR part 2634 to... 5 Administrative Personnel 3 2010-01-01 2010-01-01 false Annual ethics training for public filers. 2638.704 Section 2638.704 Administrative Personnel OFFICE OF GOVERNMENT ETHICS GOVERNMENT ETHICS...

  5. 78 FR 59662 - Annual Public Meeting of the Interagency Steering Committee on Multimedia Environmental Modeling

    Science.gov (United States)

    2013-09-27

    ...) Citizen science, crowd sourcing, and social media; (2) environmental evaluation and remediation; (3...: The annual public meeting provides an opportunity for the scientific community, ISCMEM members and... mutual interest to ISCMEM participants. The steering committee will hold an open business session on...

  6. 76 FR 30921 - International Whaling Commission; 63rd Annual Meeting; Announcement of Public Meetings

    Science.gov (United States)

    2011-05-27

    ... National Oceanic and Atmospheric Administration RIN 0648-XA459 International Whaling Commission; 63rd... annual International Whaling Commission (IWC) meeting. DATES: The public meetings will be held June 14... International Convention for the Regulation of Whaling, 1946. The U.S. Commissioner has responsibility for...

  7. 75 FR 23242 - International Whaling Commission; 62nd Annual Meeting; Announcement of Public Meetings

    Science.gov (United States)

    2010-05-03

    ... National Oceanic and Atmospheric Administration RIN 0648-XV74 International Whaling Commission; 62nd Annual... International Whaling Commission (IWC) meeting. DATES: The public meetings will be held May 20 and May 26, 2010... the Regulation of Whaling, 1946. The U.S. Commissioner has responsibility for the preparation...

  8. 77 FR 25408 - International Whaling Commission; 64th Annual Meeting; Announcement of Public Meetings

    Science.gov (United States)

    2012-04-30

    ... National Oceanic and Atmospheric Administration RIN 0648-XB150 International Whaling Commission; 64th... annual International Whaling Commission (IWC) meeting. DATES: The public meeting will be held June 5... the United States under the International Convention for the Regulation of Whaling, 1946. The U.S....

  9. 75 FR 81684 - Order Approving Public Company Accounting Oversight Board Budget and Annual Accounting Support...

    Science.gov (United States)

    2010-12-28

    ... discussions of PCAOB budget matters. \\2\\ 17 CFR 202.190. See Release No. 33-8724 (July 18, 2006) [71 FR 41998...; Release No. 34-63596/December 22, 2010] Order Approving Public Company Accounting Oversight Board Budget and Annual Accounting Support Fee for Calendar Year 2011 The Sarbanes-Oxley Act of 2002, as...

  10. The ICR142 NGS validation series: a resource for orthogonal assessment of NGS analysis.

    Science.gov (United States)

    Ruark, Elise; Renwick, Anthony; Clarke, Matthew; Snape, Katie; Ramsay, Emma; Elliott, Anna; Hanks, Sandra; Strydom, Ann; Seal, Sheila; Rahman, Nazneen

    2016-01-01

    To provide a useful community resource for orthogonal assessment of NGS analysis software, we present the ICR142 NGS validation series. The dataset includes high-quality exome sequence data from 142 samples together with Sanger sequence data at 730 sites; 409 sites with variants and 321 sites at which variants were called by an NGS analysis tool, but no variant is present in the corresponding Sanger sequence. The dataset includes 286 indel variants and 275 negative indel sites, and thus the ICR142 validation dataset is of particular utility in evaluating indel calling performance. The FASTQ files and Sanger sequence results can be accessed in the European Genome-phenome Archive under the accession number EGAS00001001332.

  11. Dry storage of irradiated CANDU fuel at Pickering NGS

    International Nuclear Information System (INIS)

    Ontario Hydro generates about 86 million MW-h/year from its 20 nuclear CANDU reactors. The combination of a large generating capacity and relatively low fuel burn-up means that Ontario Hydro must manage very large volume of its used fuel. Irradiated fuel bays at Pickering NGS will be full by mid 1995. Additional storage capacity will be required by this date for the station to continue operation. Several long term storage options to supplement existing on-site facilities were studied. The dry storage system, based on the modular storage container, as an option, was found to be economical and operationally simple. The dry storage facility at Pickering NGS is planned to provide additional on-site storage capacity for fuel generated from 1994 to the end of the station's operating life (year 2025). This paper describes the design and operation of the Dry Storage System at Ontario Hydro's Pickering Nuclear Power Generating Station. The facility is planned as a two phased project. Phase I will provide a storage space for 700 dry storage containers (268,800 fuel bundles) or about 12 years of station's operation. Phase II will have the capacity for additional 800 containers (307,200 fuel bundles) and will provide storage until station decommissioning in 2025. Seventy containers will be required annually to meet storage requirements of the station's operation at 100% capacity factor. Staff of six persons will be required to operate the facility. Normal operation includes activities such as receiving and commissioning new containers, loading them with 4 modules of used fuel in the bays, draining and drying the cavity, decontaminating the container surface and lid welding. A helium leak test is performed before the container is placed in the storage. (author) 6 refs., 6 tabs., 7 figs

  12. Gentilly - 2 NGS emergency plan

    International Nuclear Information System (INIS)

    , respectively. Relating issues such as preparedness organisation, information circulation, communication and public relations, facilities, equipment, training and exercises are also presented

  13. Scalable and cost-effective NGS genotyping in the cloud

    OpenAIRE

    Souilmi, Yassine; Lancaster, Alex K.; Jung, Jae-Yoon; Rizzo, Ettore; Hawkins, Jared B.; Powles, Ryan; Amzazi, Saaïd; Ghazal, Hassan; Peter J. Tonellato; Wall, Dennis P

    2015-01-01

    Background: While next-generation sequencing (NGS) costs have plummeted in recent years, cost and complexity of computation remain substantial barriers to the use of NGS in routine clinical care. The clinical potential of NGS will not be realized until robust and routine whole genome sequencing data can be accurately rendered to medically actionable reports within a time window of hours and at scales of economy in the 10’s of dollars. Results: We take a step towards addressing this challenge,...

  14. Remotely controlled repairs at Douglas Point NGS

    International Nuclear Information System (INIS)

    In September, 1977, leakage of heavy water at a rate of 125 kg/hr was detected in an area of the Douglas Point NGS reactor vault below the calandria known as the lower labyrinth. Radiation in the area ranges up to 5000 R/hr and the only ready access was through four 75 mm inspection ports that open into the moderator room. Remote-controlled equipment was designed and built to diagnose the problems and carry out repairs. All damaged piping was fixed, supports were replaced as needed, and system vibration was reduced. The work was done with no injuries and little radiation dose

  15. Alternate Cluster Generation for Picomolar NGS Libraries

    OpenAIRE

    Huerta, G.; Heckmann, T; Deiderick, H.; Barnette, M.; Hunicke-Smith, S.P.; Podnar, J.W.

    2014-01-01

    While advancements in library preparation techniques now enable NGS library preparation from single cells and nano- to picograms of DNA or RNA, it can still be difficult to reach Illumina's recommended final library concentration of 2 nM for DNA template preparation prior to cluster generation. This can be an acute issue for ChIP-seq, RIP-seq, and LCM-based samples. We have developed a simple and robust alternative method that maximizes number of reads from an Illumina library without having ...

  16. lociNGS: a lightweight alternative for assessing suitability of next-generation loci for evolutionary analysis.

    Directory of Open Access Journals (Sweden)

    Sarah M Hird

    Full Text Available Genomic enrichment methods and next-generation sequencing produce uneven coverage for the portions of the genome (the loci they target; this information is essential for ascertaining the suitability of each locus for further analysis. lociNGS is a user-friendly accessory program that takes multi-FASTA formatted loci, next-generation sequence alignments and demographic data as input and collates, displays and outputs information about the data. Summary information includes the parameters coverage per locus, coverage per individual and number of polymorphic sites, among others. The program can output the raw sequences used to call loci from next-generation sequencing data. lociNGS also reformats subsets of loci in three commonly used formats for multi-locus phylogeographic and population genetics analyses - NEXUS, IMa2 and Migrate. lociNGS is available at https://github.com/SHird/lociNGS and is dependent on installation of MongoDB (freely available at http://www.mongodb.org/downloads. lociNGS is written in Python and is supported on MacOSX and Unix; it is distributed under a GNU General Public License.

  17. Analysis and Visualization of ChIP-Seq and RNA-Seq Sequence Alignments Using ngs.plot.

    Science.gov (United States)

    Loh, Yong-Hwee Eddie; Shen, Li

    2016-01-01

    The continual maturation and increasing applications of next-generation sequencing technology in scientific research have yielded ever-increasing amounts of data that need to be effectively and efficiently analyzed and innovatively mined for new biological insights. We have developed ngs.plot-a quick and easy-to-use bioinformatics tool that performs visualizations of the spatial relationships between sequencing alignment enrichment and specific genomic features or regions. More importantly, ngs.plot is customizable beyond the use of standard genomic feature databases to allow the analysis and visualization of user-specified regions of interest generated by the user's own hypotheses. In this protocol, we demonstrate and explain the use of ngs.plot using command line executions, as well as a web-based workflow on the Galaxy framework. We replicate the underlying commands used in the analysis of a true biological dataset that we had reported and published earlier and demonstrate how ngs.plot can easily generate publication-ready figures. With ngs.plot, users would be able to efficiently and innovatively mine their own datasets without having to be involved in the technical aspects of sequence coverage calculations and genomic databases.

  18. Validation of OHRFSP against actual operating data from Pickering NGS A and Bruce NGS A

    International Nuclear Information System (INIS)

    The Ontario Hydro Reactor Fuelling Simulation Program (OHRFSP) is a fuel management code that does a variety of core physics and related calculations useful in the design, safety analysis, and fuel performance analysis of CANDU-PHW reactors. The program has a modular structure that allows each of its individual modules to be run separately. Modules are linked by a common database, a common control package, and common data handling routines. Comparisons between OHRFSP models of Bruce NGS A and Pickering NGS A with operating data were used to validate the OHRFSP calculations. An improved methodology was developed for estimating the channel power peaking factor and average maximum channel, and bundle powers from a small sample of random instantaneous runs

  19. Pickering NGS-A versus Pickering NGS-B: changes in commissioning techniques and their impact

    International Nuclear Information System (INIS)

    Modernization of equipment, changes in design codes and standards, and tightening of regulatory requirements have combined to make Pickering NGS-B in many ways different from its predecessor, Pickering 'A'. This paper briefly describes how a few selected commissioning techniques used to place Pickering 'A' into service were further developed to cope with the new requirements for Pickering 'B'. The relative performance of the commissioning programmes between the two stations is also compared

  20. NGS-eval: NGS Error analysis and novel sequence VAriant detection tooL

    OpenAIRE

    May, A.; Abeln, S.; Buijs, M.J.; Heringa, J.; Crielaard, W.; Brandt, B.W.

    2015-01-01

    Massively parallel sequencing of microbial genetic markers (MGMs) is used to uncover the species composition in a multitude of ecological niches. These sequencing runs often contain a sample with known composition that can be used to evaluate the sequencing quality or to detect novel sequence variants. With NGS-eval, the reads from such (mock) samples can be used to (i) explore the differences between the reads and their references and to (ii) estimate the sequencing error rate. This tool map...

  1. Analysis of Publication Status of Abstracts Presented at the Annual Meeting of the Korean Academy of Rehabilitation Medicine

    OpenAIRE

    Lim, Jae Kuk; Han, Jun Young; Lee, Hyun Cheol; Lee, Jiwon; Chung, Hong; Kim, Jong Moon; Kim, Shin Kyoung

    2013-01-01

    Objective To analyze publication rate, time to publication and the characteristics of the abstracts presented at the annual Korean Academy of Rehabilitation Medicine (KARM) meetings. Methods A total of 1,027 abstracts presented at the 2008 and 2009 annual KARM meetings were enrolled in the database and searched for their subsequent citation in PubMed, KoreaMed, and Google Scholar. Results The data analysis revealed that 317 (30.87%) abstracts, were published as full-length journal articles an...

  2. NGS-eval: NGS Error analysis and novel sequence VAriant detection tooL.

    Science.gov (United States)

    May, Ali; Abeln, Sanne; Buijs, Mark J; Heringa, Jaap; Crielaard, Wim; Brandt, Bernd W

    2015-07-01

    Massively parallel sequencing of microbial genetic markers (MGMs) is used to uncover the species composition in a multitude of ecological niches. These sequencing runs often contain a sample with known composition that can be used to evaluate the sequencing quality or to detect novel sequence variants. With NGS-eval, the reads from such (mock) samples can be used to (i) explore the differences between the reads and their references and to (ii) estimate the sequencing error rate. This tool maps these reads to references and calculates as well as visualizes the different types of sequencing errors. Clearly, sequencing errors can only be accurately calculated if the reference sequences are correct. However, even with known strains, it is not straightforward to select the correct references from databases. We previously analysed a pyrosequencing dataset from a mock sample to estimate sequencing error rates and detected sequence variants in our mock community, allowing us to obtain an accurate error estimation. Here, we demonstrate the variant detection and error analysis capability of NGS-eval with Illumina MiSeq reads from the same mock community. While tailored towards the field of metagenomics, this server can be used for any type of MGM-based reads. NGS-eval is available at http://www.ibi.vu.nl/programs/ngsevalwww/.

  3. Americans Speak out: Are Educators and Policy Makers Listening?--The 40th Annual Phi Delta Kappa/Gallup Poll of the Public's Attitudes toward the Public Schools

    Science.gov (United States)

    Bushaw, William J.; Gallup, Alec M.

    2008-01-01

    Parents, educators, political leaders--all Americans need the information contained in this article. But why this poll? First, it's not just a quick snapshot of public perception. Rather, it is a serious research tool, conducted annually, that allows Phi Delta Kappa (PDK) members and other educators and policy makers to track public opinion from…

  4. Americans Put Teacher Quality on Center Stage: The 46th Annual PDK/Gallup Poll of the Public's Attitudes toward the Public Schools. Part II

    Science.gov (United States)

    Bushaw, William J.; Calderon, Valerie J.

    2014-01-01

    In this, the second installment of a two-part report of the 46th annual PDK/Gallup Poll of the Public's Attitudes Toward the Public Schools, the report unveils what Americans are thinking about public schools--in particular their views of teachers and the classrooms where they work. Some findings were surprising, others were not. For example,…

  5. The Use of GOCE/GRACE Information in the Latest NGS xGeoid15 Model for the USA

    Science.gov (United States)

    Holmes, S. A.; Li, X.; Youngman, M.

    2015-12-01

    The U.S. National Geodetic Survey [NGS], through its Gravity for the Redefinition of the American Vertical Datum [GRAV-D] program, is flying airborne gravity surveys over the USA and its territories. By 2022, NGS intends that all orthometric heights in the USA will be determined in the field using a reliable national gravimetric geoid model to transform from geodetic heights obtained from GPS. Towards this end, all available airborne data has been incorporated into a new NGS experimental geoid model - xGEOID15. The xGEOID15 model is the second in a series of annual experimental geoid models that incorporates NGS GRAV-D airborne data. This series provides a useful benchmark for assessing and improving current techniques, to ultimately compute a geoid model that can support a national physical height system by 2022. Here, we focus on the combination of the latest GOCE/GRACE models with the terrestrial gravimetry (land/airborne) that was applied for xGeoid15. Comparisons against existing combination gravitational solutions, such as EGM2008 and EIGEN6C4, as well as recent geoid models, such as xGeoid14 and CGG2013, are interesting for what they reveal about the respective use of the GOCE/GRACE satgrav information.

  6. 77 FR 56919 - Secretarial Review and Publication of the Annual Report to Congress Submitted by the Contracted...

    Science.gov (United States)

    2012-09-14

    ... focus to explicitly include hospitals, office- based practices, ambulatory surgery centers, and skilled... publication of the annual report on September 10, 2009 (74 FR 46594). In March 2010, NQF submitted to Congress... FR 65340) to comply with the statutorily required Secretarial review and publication. In March...

  7. CRCDA--Comprehensive resources for cancer NGS data analysis.

    Science.gov (United States)

    Thangam, Manonanthini; Gopal, Ramesh Kumar

    2015-01-01

    Next generation sequencing (NGS) innovations put a compelling landmark in life science and changed the direction of research in clinical oncology with its productivity to diagnose and treat cancer. The aim of our portal comprehensive resources for cancer NGS data analysis (CRCDA) is to provide a collection of different NGS tools and pipelines under diverse classes with cancer pathways and databases and furthermore, literature information from PubMed. The literature data was constrained to 18 most common cancer types such as breast cancer, colon cancer and other cancers that exhibit in worldwide population. NGS-cancer tools for the convenience have been categorized into cancer genomics, cancer transcriptomics, cancer epigenomics, quality control and visualization. Pipelines for variant detection, quality control and data analysis were listed to provide out-of-the box solution for NGS data analysis, which may help researchers to overcome challenges in selecting and configuring individual tools for analysing exome, whole genome and transcriptome data. An extensive search page was developed that can be queried by using (i) type of data [literature, gene data and sequence read archive (SRA) data] and (ii) type of cancer (selected based on global incidence and accessibility of data). For each category of analysis, variety of tools are available and the biggest challenge is in searching and using the right tool for the right application. The objective of the work is collecting tools in each category available at various places and arranging the tools and other data in a simple and user-friendly manner for biologists and oncologists to find information easier. To the best of our knowledge, we have collected and presented a comprehensive package of most of the resources available in cancer for NGS data analysis. Given these factors, we believe that this website will be an useful resource to the NGS research community working on cancer. Database URL: http://bioinfo.au-kbc.org.in/ngs/ngshome.html.

  8. Next generation sequencing (NGS)technologies and applications

    Energy Technology Data Exchange (ETDEWEB)

    Vuyisich, Momchilo [Los Alamos National Laboratory

    2012-09-11

    NGS technology overview: (1) NGS library preparation - Nucleic acids extraction, Sample quality control, RNA conversion to cDNA, Addition of sequencing adapters, Quality control of library; (2) Sequencing - Clonal amplification of library fragments, (except PacBio), Sequencing by synthesis, Data output (reads and quality); and (3) Data analysis - Read mapping, Genome assembly, Gene expression, Operon structure, sRNA discovery, and Epigenetic analyses.

  9. NGS-Trex : next generation sequencing transcriptome profile explorer

    OpenAIRE

    I. Boria; L. Boatti; Pesole, G; F. Mignone

    2013-01-01

    Background Next-Generation Sequencing (NGS) technology has exceptionally increased the ability to sequence DNA in a massively parallel and cost-effective manner. Nevertheless, NGS data analysis requires bioinformatics skills and computational resources well beyond the possibilities of many "wet biology" laboratories. Moreover, most of projects only require few sequencing cycles and standard tools or workflows to carry out suitable analyses for the identification and annotation of genes, trans...

  10. Optimal LGS pointing with faint tip-tilt NGS

    OpenAIRE

    Dekany, Richard

    2010-01-01

    Experience with the current generation of astronomical single laser guide star (LGS) adaptive optics (AO) systems has demonstrated system performance that is often limited by residual tip-tilt errors induced by the paucity of bright tip-tilt natural guide stars (NGS). To overcome this limitation, we are developing a new generation of tip-tilt sensors that will operate at near-infrared wavelengths where the NGS is sharpened to the diffraction limit. To optimize performance, single LGS AO syste...

  11. Development of a high-resolution NGS-based HLA-typing and analysis pipeline.

    Science.gov (United States)

    Wittig, Michael; Anmarkrud, Jarl A; Kässens, Jan C; Koch, Simon; Forster, Michael; Ellinghaus, Eva; Hov, Johannes R; Sauer, Sascha; Schimmler, Manfred; Ziemann, Malte; Görg, Siegfried; Jacob, Frank; Karlsen, Tom H; Franke, Andre

    2015-06-23

    The human leukocyte antigen (HLA) complex contains the most polymorphic genes in the human genome. The classical HLA class I and II genes define the specificity of adaptive immune responses. Genetic variation at the HLA genes is associated with susceptibility to autoimmune and infectious diseases and plays a major role in transplantation medicine and immunology. Currently, the HLA genes are characterized using Sanger- or next-generation sequencing (NGS) of a limited amplicon repertoire or labeled oligonucleotides for allele-specific sequences. High-quality NGS-based methods are in proprietary use and not publicly available. Here, we introduce the first highly automated open-kit/open-source HLA-typing method for NGS. The method employs in-solution targeted capturing of the classical class I (HLA-A, HLA-B, HLA-C) and class II HLA genes (HLA-DRB1, HLA-DQA1, HLA-DQB1, HLA-DPA1, HLA-DPB1). The calling algorithm allows for highly confident allele-calling to three-field resolution (cDNA nucleotide variants). The method was validated on 357 commercially available DNA samples with known HLA alleles obtained by classical typing. Our results showed on average an accurate allele call rate of 0.99 in a fully automated manner, identifying also errors in the reference data. Finally, our method provides the flexibility to add further enrichment target regions.

  12. Orthogonal NGS for High Throughput Clinical Diagnostics.

    Science.gov (United States)

    Chennagiri, Niru; White, Eric J; Frieden, Alexander; Lopez, Edgardo; Lieber, Daniel S; Nikiforov, Anastasia; Ross, Tristen; Batorsky, Rebecca; Hansen, Sherry; Lip, Va; Luquette, Lovelace J; Mauceli, Evan; Margulies, David; Milos, Patrice M; Napolitano, Nichole; Nizzari, Marcia M; Yu, Timothy; Thompson, John F

    2016-04-19

    Next generation sequencing is a transformative technology for discovering and diagnosing genetic disorders. However, high-throughput sequencing remains error-prone, necessitating variant confirmation in order to meet the exacting demands of clinical diagnostic sequencing. To address this, we devised an orthogonal, dual platform approach employing complementary target capture and sequencing chemistries to improve speed and accuracy of variant calls at a genomic scale. We combined DNA selection by bait-based hybridization followed by Illumina NextSeq reversible terminator sequencing with DNA selection by amplification followed by Ion Proton semiconductor sequencing. This approach yields genomic scale orthogonal confirmation of ~95% of exome variants. Overall variant sensitivity improves as each method covers thousands of coding exons missed by the other. We conclude that orthogonal NGS offers improvements in variant calling sensitivity when two platforms are used, better specificity for variants identified on both platforms, and greatly reduces the time and expense of Sanger follow-up, thus enabling physicians to act on genomic results more quickly.

  13. VOLUNTARY RISK REPORTING IN ANNUAL REPORTS – CASE STUDY OF THE PRACTICES OF POLISH PUBLIC COMPANIES

    Directory of Open Access Journals (Sweden)

    Monika-Wieczorek Kosmala

    2015-05-01

    Full Text Available Grounded on literature review on risk disclosures and risk reporting practices, the paper aims to explore the extent and content of voluntary risk reporting practices of non-financial companies. Annual reports, focusing on several areas of concern of sampled public companies from three different sectors were subject to content analysis combined with morphology analysis. The results show that the extent of risk disclosure within non-financial risk exposure is broad and not connected with the sector, which is similar to findings within the cohesion of reported risk exposure with those provided in the so-called “top 10 risk” rankings. However, disclosures concerning risk management practices visibly differ among the analysed sectors. The paper contributes to the existing debate on the need and practice of risk reporting by providing insight into practices of non-financial companies operating in Poland as an emerging economy.

  14. Try It Again, Uncle Sam: The 46th Annual PDK/Gallup Poll of the Public's Attitudes toward the Public Schools. [Part I

    Science.gov (United States)

    Bushaw, William J.; Calderon, Valerie J.

    2014-01-01

    The 46th Annual PDK/Gallup Poll of the Public's Attitudes toward the Public Schools reveals that Americans say they know a lot more this year about the Common Core State Standards than they indicated a year ago, and they like it a lot less. Americans also say local school boards should have the greatest influence in deciding what is taught in…

  15. NGS-QC Generator: A Quality Control System for ChIP-Seq and Related Deep Sequencing-Generated Datasets.

    Science.gov (United States)

    Mendoza-Parra, Marco Antonio; Saleem, Mohamed-Ashick M; Blum, Matthias; Cholley, Pierre-Etienne; Gronemeyer, Hinrich

    2016-01-01

    The combination of massive parallel sequencing with a variety of modern DNA/RNA enrichment technologies provides means for interrogating functional protein-genome interactions (ChIP-seq), genome-wide transcriptional activity (RNA-seq; GRO-seq), chromatin accessibility (DNase-seq, FAIRE-seq, MNase-seq), and more recently the three-dimensional organization of chromatin (Hi-C, ChIA-PET). In systems biology-based approaches several of these readouts are generally cumulated with the aim of describing living systems through a reconstitution of the genome-regulatory functions. However, an issue that is often underestimated is that conclusions drawn from such multidimensional analyses of NGS-derived datasets critically depend on the quality of the compared datasets. To address this problem, we have developed the NGS-QC Generator, a quality control system that infers quality descriptors for any kind of ChIP-sequencing and related datasets. In this chapter we provide a detailed protocol for (1) assessing quality descriptors with the NGS-QC Generator; (2) to interpret the generated reports; and (3) to explore the database of QC indicators (www.ngs-qc.org) for >21,000 publicly available datasets.

  16. Steam generator tube fretting - Darlington NGS experience

    International Nuclear Information System (INIS)

    Early signs of tube fretting in the U-bend region of Darlington NGS Steam Generators (SGs) were observed during the metallurgical examination of the removed peripheral tube U-bend sections from Unit 4 SG3 in 1995. During a forced outage in early 1998, Eddy Current (ECT) tube inspections in Unit 2 SG4 revealed more extensive fretting of the tubes at the U-bend AVB support locations. Subsequently in the period of 1999-2001, planned Eddy Current tube inspections have been carried out in all units covering all SGs. These inspections have revealed considerable U-bend tube fretting with a number of these fret depths in excess of 40% tw. Evaluation of the ECT and UT results, in conjunction with engineering assessment of the SG design and construction, have determined tube fretting in the U-bend region as an active and reportable degradation mechanism in these SGs. To date, all 16 Darlington SGs have undergone a major ECT inspection. In these inspections as a minimum, the identified fretting region of the U-bend has been adequately covered. Analyses of the inspection results have been carried out to provide trends and observations of the fretting in the U-bend. These showed the fretted U-bend tubes to be localized in the area bounded by Rows 70 and above, and Columns 39 to 83 which has been defined as the 'Area at Risk' of U-bend fretting for Darlington SGs. In the distribution of the frets at the U-bend support locations, they showed a strong biasing of the fretting towards the cold leg supports with the mean centered a third the way between CU4 and CU3. A general understanding of the 'Root Cause of Fretting' shows it to be associated with tube clearance, which invariably results and acts together with conditions of insufficient support preload. While the fretting by tube tends to exhibit a certain degree of randomness, the fretting remains localized to the 'Area at Risk'. This offers a unique opportunity of localized corrective measures that are both simpler in design

  17. Current Rates of Publication for Podium and Poster Presentations at the American Society for Surgery of the Hand Annual Meetings

    Directory of Open Access Journals (Sweden)

    Joshua M. Abzug

    2014-09-01

     Currently, fewer than half of the studies presented at Annual Meetings of the ASSH achieve publication in peer-eviewed journals. Presentations are most likely to be published within 3 years, and almost half are published in Journal of Hand Surgery . 

  18. 77 FR 58205 - Request for Public Comments on Annual Review of Country Eligibility for Benefits Under the...

    Science.gov (United States)

    2012-09-19

    ... From the Federal Register Online via the Government Publishing Office OFFICE OF THE UNITED STATES TRADE REPRESENTATIVE Request for Public Comments on Annual Review of Country Eligibility for Benefits... to reduce poverty; a system to combat corruption and bribery; and protection of...

  19. 78 FR 60367 - Request for Public Comments on Annual Review of Country Eligibility for Benefits Under the...

    Science.gov (United States)

    2013-10-01

    ... From the Federal Register Online via the Government Publishing Office OFFICE OF THE UNITED STATES TRADE REPRESENTATIVE Request for Public Comments on Annual Review of Country Eligibility for Benefits... corruption and bribery; and protection of internationally recognized worker rights. In addition, the...

  20. Genetic counselors' (GC) knowledge, awareness, understanding of clinical next-generation sequencing (NGS) genomic testing.

    Science.gov (United States)

    Boland, P M; Ruth, K; Matro, J M; Rainey, K L; Fang, C Y; Wong, Y N; Daly, M B; Hall, M J

    2015-12-01

    Genomic tests are increasingly complex, less expensive, and more widely available with the advent of next-generation sequencing (NGS). We assessed knowledge and perceptions among genetic counselors pertaining to NGS genomic testing via an online survey. Associations between selected characteristics and perceptions were examined. Recent education on NGS testing was common, but practical experience limited. Perceived understanding of clinical NGS was modest, specifically concerning tumor testing. Greater perceived understanding of clinical NGS testing correlated with more time spent in cancer-related counseling, exposure to NGS testing, and NGS-focused education. Substantial disagreement about the role of counseling for tumor-based testing was seen. Finally, a majority of counselors agreed with the need for more education about clinical NGS testing, supporting this approach to optimizing implementation.

  1. NGS Catalog: A Database of Next Generation Sequencing Studies in Humans

    OpenAIRE

    Xia, Junfeng; Wang, Qingguo; Jia, Peilin; Wang, Bing; Pao, William; Zhao, Zhongming

    2012-01-01

    Next generation sequencing (NGS) technologies have been rapidly applied in biomedical and biological research since its advent only a few years ago, and they are expected to advance at an unprecedented pace in the following years. To provide the research community with a comprehensive NGS resource, we have developed the database Next Generation Sequencing Catalog (NGS Catalog, http://bioinfo.mc.vanderbilt.edu/NGS/index.html), a continually updated database that collects, curates and manages a...

  2. Evolution of CANDU vacuum building and pressure relief structures from Pickering NGS A to Darlington NGS A

    International Nuclear Information System (INIS)

    The vacuum building (VB) and pressure relief structures (PRS) are the unique features of multiple unit CANDU containments. In case of loss-of-coolant accident, the released radionuclides are drawn through the PRS into the subatmospheric VB, doused and contained without being released to the environment. This paper describes the differences in design, configuration and layout of the VB and PRS from Pickering NGS A to Darlington NGS A due to new developments in design concepts and to requirements which have proceeded from the experience gained in both the design and operation of the nuclear stations. (orig.)

  3. Comparison of Pickering NGS performance with world power reactors, 1977

    International Nuclear Information System (INIS)

    Pickering NGS performance is compared, in highly graphic form, with the perfomance of other nuclear power plants around the world. The four Pickering reactors score in the top six, rated by gross capacity factor. Major system suppliers for world power reactors above 500 MW are cataloged. (E.C.B.)

  4. Make Teaching More Professional: The 47th Annual PDK/Gallup Poll of the Public's Attitudes toward the Public Schools

    Science.gov (United States)

    Starr, Joshua P.

    2015-01-01

    Americans want higher professional requirements for teachers and believe teacher pay is too low, but they don't like tenure, according to the newest PDK/Gallup Poll of the Public's Attitudes To­ward the Public Schools. Public school parents trust and have confidence in the nation's teachers, and they said communicating with their child's teacher…

  5. AECB staff review of Pickering NGS operations for the year 1988

    International Nuclear Information System (INIS)

    The operation of Pickering NGS-A Units 1-4 and Pickering NGS-B Units 5-8 are monitored to ensure compliance with licensing requirements by the AECB Pickering project office staff. This report presents AECB staff's review of major licensing issues and of the operational performance of Pickering NGS during 1988. The report is limited to those aspects that AECB staff considers to have particular safety significance. More detailed information on routine performance is contained in Ontario Hydro's 1988 Quarterly Technical Reports for Pickering NGS-A and Pickering NGS-B

  6. Ontario Hydro nuclear generation division information systems at Darlington NGS

    International Nuclear Information System (INIS)

    The need for a more efficient method of managing information in Ontario Hydro's nuclear stations was initially identified in the early 1980's. There followed a Nuclear Generation Division Information System Planning Study in 1982, which identified the major information problems confronting the division and specified the existing and new information systems required to address these. In 1983/84, the requirements for these systems were defined. Detailed specifications were produced in 1985 and during 1986/87 the systems were designed, tested and installed, initially at Darlington NGS. The systems are now in full production at Darlington and are in the process of being installed at Pickering NGS. This paper outlines an overview of these systems, a brief description of the Work Management System, the way in which they are all used, the costs associated with them and the benefits resulting from them

  7. TUF validation against LRV test data at Darlington NGS

    International Nuclear Information System (INIS)

    To obtain the characteristics of the heat transport control valves and to examine the interaction between the heat transport system and the feed and bleed system, a test on the liquid relief valves (LRV) failed open event was performed in Unit 1 of Darlington NGS in 1993. Since TUF has been used to support the short term modifications to the process control system at Darlington NGS, the validation of TUF against this test is required. In this paper, the comparisons between the TUF predictions and the plant data are reported. It is concluded that the TUF predictions are in good agreement with station measured data. The code can therefore be used with confidence in support of the modifications for LRV fail open events. (author)

  8. Multiple Object Adaptive Optics: Mixed NGS/LGS tomography

    Science.gov (United States)

    Morris, Tim; Gendron, Eric; Basden, Alastair; Martin, Olivier; Osborn, James; Henry, David; Hubert, Zoltan; Sivo, Gaetano; Gratadour, Damien; Chemla, Fanny; Sevin, Arnaud; Cohen, Matthieu; Younger, Eddy; Vidal, Fabrice; Wilson, Richard; Batterley, Tim; Bitenc, Urban; Reeves, Andrew; Bharmal, Nazim; Raynaud, Henri-François; Kulcsar, Caroline; Conan, Jean-Marc; Guzman, Dani; De Cos Juez, Javier; Huet, Jean-Michel; Perret, Denis; Dickson, Colin; Atkinson, David; Baillie, Tom; Longmore, Andy; Todd, Stephen; Talbot, Gordon; Morris, Simon; Myers, Richard; Rousset, Gérard

    2013-12-01

    Open-loop adaptive optics has been successfully demonstrated on-sky by several groups, including the fully tomographic MOAO demonstration made using CANARY. MOAO instrumentation such as RAVEN will deliver the first astronomical science and other planned instruments aim to extend both open-loop AO performance and the number of corrected fields. Many of these planned systems rely on the use of tomographic open-loop LGS wavefront sensing. Here we present results from the combined NGS/LGS tomographic CANARY system and then compare the NGS- and LGS-based tomographic system performance. We identify the major system performance drivers, and highlight some potential routes for further exploitation of open-loop tomographic AO.

  9. Using Annual Data to Estimate the Public Health Impact of Extreme Temperatures.

    Science.gov (United States)

    Goggins, William B; Yang, Chunyuh; Hokama, Tomiko; Law, Lewis S K; Chan, Emily Y Y

    2015-07-01

    Short-term associations between both hot and cold ambient temperatures and higher mortality have been found worldwide. Few studies have examined these associations on longer time scales. Age-standardized mortality rates (ASMRs) were calculated for 1976-2012 for Hong Kong SAR, People's Republic of China, defining "annual" time periods in 2 ways: from May through April of the following year and from November through October. Annual frequency and severity of extreme temperatures were summarized by using a degree-days approach with extreme heat expressed as annual degree-days >29.3°C and cold as annual degree-days ASMR, with adjustment for long-term trends. Increases of 10 hot or 200 cold degree-days in an annual period, the approximate interquartile ranges for these variables, were significantly (all P's ≤ 0.011) associated with 1.9% or 3.1% increases, respectively, in the annual ASMR for the May-April analyses and with 2.2% or 2.8% increases, respectively, in the November-October analyses. Associations were stronger for noncancer and elderly mortality. Mortality increases associated with extreme temperature are not simply due to short-term forward displacement of deaths that would have occurred anyway within a few weeks.

  10. Recent operating experience with 28 element fuel at Pickering NGS

    International Nuclear Information System (INIS)

    A review of 28-element fuel operating experience at Pickering NGS is presented. The following topics are discussed: 1. Recent experience with in-core defects and 131I releases; 2. Operating strategies to minimize defect potential or to mitigate 131I releases to the primary heat transport system; 3. Impact of reduced regulatory limits as well as higher corporate expectations on operating strategies. 3 refs., 3 figs., 2 tabs

  11. Leakage from biological shield cooling system in Pickering NGS A

    International Nuclear Information System (INIS)

    Over the past eight years, a number of leaks have developed in the Biological Shield Cooling (BSC) system of the Pickering NGS A reactors. The highest leak rate exists in Unit 4. The failure mechanism is not known, but corrosion and/or weld failure are suspected. This paper summarizes the concerns associated with the leaks and possible solutions. It should be noted that the BSC system is peculiar to Pickering A reactors only

  12. COV2HTML: a visualization and analysis tool of bacterial next generation sequencing (NGS) data for postgenomics life scientists.

    Science.gov (United States)

    Monot, Marc; Orgeur, Mickael; Camiade, Emilie; Brehier, Clément; Dupuy, Bruno

    2014-03-01

    COV2HTML is an interactive web interface, which is addressed to biologists, and allows performing both coverage visualization and analysis of NGS alignments performed on prokaryotic organisms (bacteria and phages). It combines two processes: a tool that converts the huge NGS mapping or coverage files into light specific coverage files containing information on genetic elements; and a visualization interface allowing a real-time analysis of data with optional integration of statistical results. To demonstrate the scope of COV2HTML, the program was tested with data from two published studies. The first data were from RNA-seq analysis of Campylobacter jejuni, based on comparison of two conditions with two replicates. We were able to recover 26 out of 27 genes highlighted in the publication using COV2HTML. The second data comprised of stranded TSS and RNA-seq data sets on the Archaea Sulfolobus solfataricus. COV2HTML was able to highlight most of the TSSs from the article and allows biologists to visualize both TSS and RNA-seq on the same screen. The strength of the COV2HTML interface is making possible NGS data analysis without software installation, login, or a long training period. A web version is accessible at https://mmonot.eu/COV2HTML/ . This website is free and open to users without any login requirement.

  13. Next generation sequencing (NGS): a golden tool in forensic toolkit.

    Science.gov (United States)

    Aly, S M; Sabri, D M

    2015-01-01

    The DNA analysis is a cornerstone in contemporary forensic sciences. DNA sequencing technologies are powerful tools that enrich molecular sciences in the past based on Sanger sequencing and continue to glowing these sciences based on Next generation sequencing (NGS). Next generation sequencing has excellent potential to flourish and increase the molecular applications in forensic sciences by jumping over the pitfalls of the conventional method of sequencing. The main advantages of NGS compared to conventional method that it utilizes simultaneously a large number of genetic markers with high-resolution of genetic data. These advantages will help in solving several challenges such as mixture analysis and dealing with minute degraded samples. Based on these new technologies, many markers could be examined to get important biological data such as age, geographical origins, tissue type determination, external visible traits and monozygotic twins identification. It also could get data related to microbes, insects, plants and soil which are of great medico-legal importance. Despite the dozens of forensic research involving NGS, there are requirements before using this technology routinely in forensic cases. Thus, there is a great need to more studies that address robustness of these techniques. Therefore, this work highlights the applications of forensic sciences in the era of massively parallel sequencing.

  14. Opportunities in public sector procurement: the national procurement service annual survey 2012

    OpenAIRE

    Flynn, Anthony; Davis, Paul; McKevitt, David; McEvoy, Emma

    2012-01-01

    The contribution that public procurement can make to expenditure reduction and the stimulation of economic growth is the focus of increasing policy attention. Driven by budgetary pressures the State has set about reducing its public procurement bill through more astute purchasing. Not just in cost cutting, however, has public procurement a role to play in Ireland’s economic recovery. By making itself more sensitive to the needs of micro and small enterprises, public procurement can inject dem...

  15. Inference of Markovian Properties of Molecular Sequences from NGS Data and Applications to Comparative Genomics

    OpenAIRE

    Ren, Jie; Song, Kai; Deng, Minghua; Reinert, Gesine; Cannon, Charles H.; Sun, Fengzhu

    2015-01-01

    Next Generation Sequencing (NGS) technologies generate large amounts of short read data for many different organisms. The fact that NGS reads are generally short makes it challenging to assemble the reads and reconstruct the original genome sequence. For clustering genomes using such NGS data, word-count based alignment-free sequence comparison is a promising approach, but for this approach, the underlying expected word counts are essential. A plausible model for this underlying distribution ...

  16. Applications of NGS : Regulatory actions of lincRNAs and SNP analysis of Restless legs syndrome

    OpenAIRE

    Albrecht, Nadine

    2013-01-01

    The advent of Next Generation Sequencing (NGS) technology makes the generation of sequence data for whole genomes and exomes possible. New challenges in Bioinformatics arise with more and more NGS data. In this work we focus on two applications of NGS: (i) Regulatory actions of large intergenic noncoding RNAs (lincRNAs) and (ii) SNP analysis of Restless legs syndrome (RLS). The most promising finding of this dissertation is the confirmation that alternative splice variants could be explained ...

  17. NgsRelate: a software tool for estimating pairwise relatedness from next-generation sequencing data

    OpenAIRE

    Korneliussen, Thorfinn Sand; Moltke, Ida

    2015-01-01

    MOTIVATION: Pairwise relatedness estimation is important in many contexts such as disease mapping and population genetics. However, all existing estimation methods are based on called genotypes, which is not ideal for next-generation sequencing (NGS) data of low depth from which genotypes cannot be called with high certainty.RESULTS: We present a software tool, NgsRelate, for estimating pairwise relatedness from NGS data. It provides maximum likelihood estimates that are based on genotype lik...

  18. The NGS WikiBook: a dynamic collaborative online training effort with long-term sustainability

    OpenAIRE

    Li, J.-W.; Bolser, D.; Schneider, M. V.; Manske, M.; Giorgi, F. M.; Vyahhi, N.; Usadel, B.; Clavijo, B. J.; Chan, T. -F.; Wong, N.; Zerbino, D.

    2013-01-01

    Next-generation sequencing (NGS) is increasingly being adopted as the backbone of biomedical research. With the commercialization of various affordable desktop sequencers, NGS will be reached by increasing numbers of cellular and molecular biologists, necessitating community consensus on bioinformatics protocols to tackle the exponential increase in quantity of sequence data. The current resources for NGS informatics are extremely fragmented. Finding a centralized synthesis is difficult. A mu...

  19. Normal and Compound Poisson Approximations for Pattern Occurrences in NGS Reads

    OpenAIRE

    Zhai, Zhiyuan; Reinert, Gesine; Song, Kai; Waterman, Michael S.; Luan, Yihui; Sun, Fengzhu

    2012-01-01

    Next generation sequencing (NGS) technologies are now widely used in many biological studies. In NGS, sequence reads are randomly sampled from the genome sequence of interest. Most computational approaches for NGS data first map the reads to the genome and then analyze the data based on the mapped reads. Since many organisms have unknown genome sequences and many reads cannot be uniquely mapped to the genomes even if the genome sequences are known, alternative analytical methods are needed fo...

  20. Impact of Next-Generation Sequencing (NGS) technology on cardiovascular disease research

    OpenAIRE

    Xu, Fengping; Wang, Qin; Zhang, Fangfang; Zhu, Yinling; Gu, Qingquan; Wu, Liping; Yang, Lin; Yang,Xu

    2012-01-01

    In recent years, hundreds of gene loci associated with multiple cardiovascular pathologies and traits have been identified through high-throughput Next-Generation Sequencing (NGS) technology. Due to the increasing efficiency and decreasing cost of NGS, rapid progresses anticipated in the field of CVD research. This review summarizes the main strategies of CV research with NGS at the level of genomics, transcriptomics, epigenetics, and proteomics.

  1. An NGS Workflow Blueprint for DNA Sequencing Data and Its Application in Individualized Molecular Oncology

    OpenAIRE

    Jian Li; Aarif Mohamed Nazeer Batcha; Björn Grüning; Mansmann, Ulrich R.

    2016-01-01

    Next-generation sequencing (NGS) technologies that have advanced rapidly in the past few years possess the potential to classify diseases, decipher the molecular code of related cell processes, identify targets for decision-making on targeted therapy or prevention strategies, and predict clinical treatment response. Thus, NGS is on its way to revolutionize oncology. With the help of NGS, we can draw a finer map for the genetic basis of diseases and can improve our understanding of diagnostic ...

  2. An NGS Workflow Blueprint for DNA Sequencing Data and Its Application in Individualized Molecular Oncology.

    Science.gov (United States)

    Li, Jian; Batcha, Aarif Mohamed Nazeer; Grüning, Björn; Mansmann, Ulrich R

    2015-01-01

    Next-generation sequencing (NGS) technologies that have advanced rapidly in the past few years possess the potential to classify diseases, decipher the molecular code of related cell processes, identify targets for decision-making on targeted therapy or prevention strategies, and predict clinical treatment response. Thus, NGS is on its way to revolutionize oncology. With the help of NGS, we can draw a finer map for the genetic basis of diseases and can improve our understanding of diagnostic and prognostic applications and therapeutic methods. Despite these advantages and its potential, NGS is facing several critical challenges, including reduction of sequencing cost, enhancement of sequencing quality, improvement of technical simplicity and reliability, and development of semiautomated and integrated analysis workflow. In order to address these challenges, we conducted a literature research and summarized a four-stage NGS workflow for providing a systematic review on NGS-based analysis, explaining the strength and weakness of diverse NGS-based software tools, and elucidating its potential connection to individualized medicine. By presenting this four-stage NGS workflow, we try to provide a minimal structural layout required for NGS data storage and reproducibility.

  3. Publication rates of presentations at the Irish Orthopaedic Association annual meeting.

    LENUS (Irish Health Repository)

    O'Neill, B J

    2014-03-01

    Presentation of scientific research at national and international meetings is an important forum for the dissemination of knowledge. Subsequent publication of a full-text paper in a peer-reviewed journal is the expected outcome of such presentations. The publication rate from these meetings is highly variable.

  4. Strategic Plan to Ensure Racial and Ethnic Diversity in Connecticut Public Higher Education, 2011. Annual Report

    Science.gov (United States)

    Connecticut Department of Higher Education (NJ1), 2011

    2011-01-01

    Increasing the participation of minority groups at public colleges and universities is a longstanding goal of the Board of Governors for Higher Education, as first outlined in its 1983 "Strategic Plan to Ensure Racial and Ethnic Diversity in Connecticut Public Higher Education." The minority groups defined by the plan are: Hispanic/Latino,…

  5. Strategic Plan to Ensure Racial and Ethnic Diversity in Connecticut Public Higher Education, 2009. Annual Report

    Science.gov (United States)

    Connecticut Department of Higher Education (NJ1), 2009

    2009-01-01

    Increasing the participation of minority groups at public colleges and universities is a longstanding goal of the Board of Governors for Higher Education, as first outlined in its 1983 "Strategic Plan to Ensure Racial and Ethnic Diversity in Connecticut Public Higher Education". The minority groups defined by the plan are: Hispanic/Latino, African…

  6. Strategic Plan to Ensure Racial and Ethnic Diversity in Connecticut Public Higher Education, 2010. Annual Report

    Science.gov (United States)

    Connecticut Department of Higher Education (NJ1), 2010

    2010-01-01

    Increasing the participation of minority groups at public colleges and universities is a longstanding goal of the Board of Governors for Higher Education, as first outlined in its 1983 "Strategic Plan to Ensure Racial and Ethnic Diversity in Connecticut Public Higher Education". The minority groups defined by the plan are: Hispanic/Latino, African…

  7. Strategic Plan to Ensure Racial and Ethnic Diversity in Connecticut Public Higher Education, 2008. Annual Report

    Science.gov (United States)

    Connecticut Department of Higher Education (NJ1), 2008

    2008-01-01

    Increasing the participation of minority groups at public colleges and universities is a longstanding goal of the Board of Governors for Higher Education, as first outlined in its 1983 "Strategic Plan to Ensure Racial and Ethnic Diversity in Connecticut Public Higher Education." The minority groups defined by the plan are: Hispanic/Latino,…

  8. Fire fighting capability assessment program Bruce B NGS

    International Nuclear Information System (INIS)

    This is a report on the completion of work relating to the assessment of the capability of Bruce B NGS to cope with a large fire incident. This included an evaluation of an exercise scenario that would simulate a large fire incident and of their fire plans and procedures. Finally the execution of fire plans by Bruce B NGS, as demonstrated by their application of human and material resources during a simulated large fire, was observed. The fire fighting equipment and the personal protective clothing and associated equipment that was in use was all of good quality and in good condition. There had also been notable improvement in communications equipment. Similarly, the human resources that had been assigned to fire fighting and rescue crews and that were available were more than adequate. Use of a logical incident command system, and the adoption of proper policy and procedures for radio communications were equally significant improvements. Practice should correct the breakdowns that occurred in these areas during the exercise. As well, there remains a need for the development of policy on fire fighting and rescue operations with more depth and clarity. In summary, the key point to be recognized is the degree of improvement that has been realized since the previous evaluation in 1990. Clearly the Emergency Response Teams organization of Bruce B NGS is evolving into an effective fire fighting force. Providing that the deficiencies identified in this report are addressed satisfactorily, Fire Cross is confident that the organization will have the capability to provide rescue and fire fighting services that will satisfy the need. 2 figs

  9. Overview of Pickering NGS Units 1 and 2 retube outage

    International Nuclear Information System (INIS)

    After the Pickering NGS Unit 2 pressure tube failure in August 1983, a decision was made in March 1984 to replace all the pressure tubes from both Unit 1 and Unit 2. This retubing was accomplished over a three year period, together with a program of inspection, maintenance, and modification on other systems. Equipment was put in a short term mothballed state at the commencement of the outage, and a thorough recommissioning of all systems, both old and new, was carried out during restart. This paper provides an overview of the program, highlighting successes and difficulties, and recommending changes for future retubing projects

  10. Revised delayed neutron data for Pickering NGS B

    International Nuclear Information System (INIS)

    Revised delayed neutron fractions and constants were calculated specifically for Pickering NGS B using the latest available delayed neutron data for fissionable isotopes, the currently recommended CANDU delayed photoneutron data, and the formulae provided by Laughton. Burnup-dependent number densities of the fissionable isotopes were computed with WIMS-AECL-IST. Validation and assessment of the new fractions and constants was performed by comparison of historical operating data with point kinetics simulation using the new values. Recommendations are made regarding possible improvements to the delayed neutron data. (author)

  11. Deaerator transient at Bruce NGS 'B' Unit 8

    International Nuclear Information System (INIS)

    On April 25, 1987 during a planned 800 MW(e) load rejection test on Unit 8, a violent transient occurred in the deaerator/deaerator storage tank which caused the 500 ton vessel-piping network to tear away from its supports and move approximately three inches in an axial direction. Considerable effort was brought to bear and within one week the unit was returned to service. This paper describes the physical nature of the event and the role of the Bruce NGS 'B' training simulator in developing and testing control modifications to prevent its recurrence. (author). 4 refs., 6 figs

  12. Bruce NGS B U-bend support stabilization

    International Nuclear Information System (INIS)

    The steam generators at Bruce NGS B have experienced a degree of tube fretting at the U-bend scalloped bar support locations. Investigation attributed the tube fretting to flow induced vibration induced wear as a result of U-bend supports which were too widely spaced (compared to current criteria), and insufficiently rigid. The paper describes the problem, the development of a stabilization configuration, its qualification, its installation tooling and procedures, and the installation of the initial trial assemblies. 4 refs., 9 figs

  13. Understanding the Basics of NGS: From Mechanism to Variant Calling

    OpenAIRE

    Muzzey, Dale; Evans, Eric A.; Lieber, Caroline

    2015-01-01

    Identifying disease-causing mutations in DNA has long been the goal of genetic medicine. In the last decade, the toolkit for discovering DNA variants has undergone rapid evolution: mutations that were historically discovered by analog approaches like Sanger sequencing and multiplex ligation-dependent probe amplification (“MLPA”) can now be decoded from a digital signal with next-generation sequencing (“NGS”). Given the explosive growth of NGS-based tests in the clinic, it is of the utmost imp...

  14. Annual report of the regional public health authorities in the Slovak Republic. Year 2012

    International Nuclear Information System (INIS)

    A brief account of activities carried out by the regional public health authorities of the Public Health Authority of the Slovak Republic (UVZ SR) in 2012 is presented. These activities are reported under the headings: (1) The environment; (2) Preventive occupational medicine; (3) Hygiene of nutrition, food safety and cosmetic products; (4) Hygiene of children and youth; (5) Epidemiology; (6) Objectivization of factors of living conditions; (7) Medical microbiology; (8) Health promotion; (9) Health protection against radiation; (10) Complaints and petitions; (11) Medical informatics and biostatistics; (12) Lectures and publications.

  15. Annual report of the regional public health authorities in the Slovak Republic. Year 2011

    International Nuclear Information System (INIS)

    A brief account of activities carried out by the regional public health authorities of the Public Health Authority of the Slovak Republic (UVZ SR) in 2011 is presented. These activities are reported under the headings: (1) The environment; (2) Preventive occupational medicine; (3)Hygiene of nutrition, food safety and cosmetic products; (4) Hygiene of children and youth; (5) Epidemiology; (6) Objectivization of factors of living conditions; (7) Medical microbiology; (8) Health promotion; (9) Health protection against radiation; (10) Complaints and petitions; (11) Medical informatics and biostatistics; (12) Lectures and publications.

  16. A slow death or eternal life? Future directions for annual public health reports.

    Science.gov (United States)

    Bruce, N; Price, C; Whincup, P

    1994-11-01

    Several years after their reintroduction, district public health reports continue to face questions about purpose, content and evaluation. As public health embraces the wider vision of the World Health Organisation's 'Health for All' strategy, and the consequent need for collaboration with other sectors and participation with communities, there is a need for the reports to respond accordingly. This theme is explored in relation to the needs of the report's diverse audience, the type of local research required to make the report relevant and effective, and approaches to evaluation. A growing local research function has resource and training implications for Departments of Public Health. The demands of producing a report every year suggest that biennial publication of the main report, with other targeted products over the intervening period, would be a more realistic and effective option. PMID:7997494

  17. NGS WFSs module for MAORY at E-ELT

    Science.gov (United States)

    Esposito, S.; Agapito, G.; Antichi, J.; Bonanno, A.; Carbonaro, L.; Giordano, C.; Spanò, P.

    We report on the natural guide star (NGS) wavefront sensors (WFS) module for MAORY, the multi-cojugate adaptive optics (MCAO) system for the ESO E-ELT. Three low-order, near-infrared (H-band), Shack-Hartmann sensors provide fast acquisition of the first 5 modes (tip, tilt, focus, astigmatism) on 3 natural guide stars over a 160 arcsec field of view. Three moderate-order (20x20), visible (600-800 nm), pyramid WFSs provide the slow Truth sensing to correct LGS wavefront estimates of low-order modes. These sensors are mounted onto three R-theta stages to patrol the field of view. The module is also equipped with a retractable, on-axis, high-order (80x80), visible, pyramid WFS for the single-conjugate AO (SCAO) mode of MAORY and MICADO. The visible WFSs share the same 80x80 pyramid WFS design. This choice enables also a MCAO NGS capability. Simulations show that Strehl ratios (SR) over 40% are reached with MCAO and three, 2x2 sub-apertures, NIR low-order WFSs working with H-mag=20 reference stars. In SCAO mode, 90% SR for a 8mag stars with a contrast down to 10-5, and 45% SR for a 16mag star, are achieved.

  18. HCV genotyping from NGS short reads and its application in genotype detection from HCV mixed infected plasma.

    Science.gov (United States)

    Qiu, Ping; Stevens, Richard; Wei, Bo; Lahser, Fred; Howe, Anita Y M; Klappenbach, Joel A; Marton, Matthew J

    2015-01-01

    Genotyping of hepatitis C virus (HCV) plays an important role in the treatment of HCV. As new genotype-specific treatment options become available, it has become increasingly important to have accurate HCV genotype and subtype information to ensure that the most appropriate treatment regimen is selected. Most current genotyping methods are unable to detect mixed genotypes from two or more HCV infections. Next generation sequencing (NGS) allows for rapid and low cost mass sequencing of viral genomes and provides an opportunity to probe the viral population from a single host. In this paper, the possibility of using short NGS reads for direct HCV genotyping without genome assembly was evaluated. We surveyed the publicly-available genetic content of three HCV drug target regions (NS3, NS5A, NS5B) in terms of whether these genes contained genotype-specific regions that could predict genotype. Six genotypes and 38 subtypes were included in this study. An automated phylogenetic analysis based HCV genotyping method was implemented and used to assess different HCV target gene regions. Candidate regions of 250-bp each were found for all three genes that have enough genetic information to predict HCV genotypes/subtypes. Validation using public datasets shows 100% genotyping accuracy. To test whether these 250-bp regions were sufficient to identify mixed genotypes, we developed a random primer-based method to sequence HCV plasma samples containing mixtures of two HCV genotypes in different ratios. We were able to determine the genotypes without ambiguity and to quantify the ratio of the abundances of the mixed genotypes in the samples. These data provide a proof-of-concept that this random primed, NGS-based short-read genotyping approach does not need prior information about the viral population and is capable of detecting mixed viral infection.

  19. Program management for spring location and repositioning (SLAR) operations at Pickering NGS

    International Nuclear Information System (INIS)

    In 1988 a major project was planned for Units 5 and 6 of Pickering NGS (Nuclear Generating Station). The project involved remotely locating and repositioning the four spacer (garter) springs separating each pressure tube from the surrounding calandria tube. This paper describes the requirements for SLAR, and the work being undertaken at Pickering NGS to implement the project

  20. Annual report of the regional public health authorities in the Slovak Republic. Year 2010

    International Nuclear Information System (INIS)

    A brief account of activities carried out by the regional public health authorities of the Public Health Authority of the Slovak Republic (UVZ SR) in 2010 is presented. These activities are reported under the headings: (1) The environment; (2) Preventive occupational medicine; (3)Hygiene of nutrition, food safety and cosmetic products; (4) Hygiene of children and youth; (5) Epidemiology; (6) Objectivization of factors of living conditions; (7) Medical microbiology; (8) Health promotion; (9) Health protection against radiation; (10) Complaints and petitions; (11) Medical informatics and biostatistics.

  1. Annual report of the Public Health Authority of the Slovak Republic in 2012

    International Nuclear Information System (INIS)

    A brief account of activities carried out by the Public Health Authority of the Slovak Republic (UVZ SR) in 2012 is presented. These activities are reported under the headings: (1) General part; (2) Department of Legislation and Law; (3) Department inspection, supervision and complaints; (4) Department of International Relations; (5) Department of Environmental Health; (6) Department of Preventive Occupational Medicine; (7) Department of food hygiene, food safety and cosmetic products; (8) Department of Hygiene of Children and Youth; (9) Department of objectification factors welfare; (10) Department of Medical Microbiology; (11) Department of Epidemiology; (12) Department of Health Promotion; (13) Department of health protection against radiation; (14) Publications and Lectures.

  2. Clinical validation of NGS technology for HLA: An early adopter's perspective.

    Science.gov (United States)

    Weimer, Eric T

    2016-10-01

    Clinical validation of NGS for HLA typing has been a topic of interest with many laboratories investigating the merits. NGS has proven effective at reducing ambiguities and costs while providing more detailed information on HLA genes not previously sequenced. The ability of NGS to multiplex many patients within a single run presents unique challenges and sequencing new regions of HLA genes requires application of our knowledge of genetics to accurately determine HLA typing. This review represents my laboratory's experience in validation of NGS for HLA typing. It describes the obstacles faced with validation of NGS and is broken down into pre-analytic, analytic, and post-analytic challenges. Each section includes solutions to address them.

  3. Two Methods for High-Throughput NGS Template Preparation for Small and Degraded Clinical Samples Without Automation

    OpenAIRE

    Kamberov, E.; Tesmer, T.; Mastronardi, M.; Langmore, John

    2012-01-01

    Clinical samples are difficult to prepare for NGS, because of the small amounts or degraded states of formalin-fixed tissue, plasma, urine, and single-cell DNA. Conventional whole genome amplification methods are too biased for NGS applications, and the existing NGS preparation kits require intermediate purifications and excessive time to prepare hundreds of samples in a day without expensive automation. We have tested two 96-well manual methods to make NGS templates from FFPE tissue, plasma,...

  4. Normal and compound poisson approximations for pattern occurrences in NGS reads.

    Science.gov (United States)

    Zhai, Zhiyuan; Reinert, Gesine; Song, Kai; Waterman, Michael S; Luan, Yihui; Sun, Fengzhu

    2012-06-01

    Next generation sequencing (NGS) technologies are now widely used in many biological studies. In NGS, sequence reads are randomly sampled from the genome sequence of interest. Most computational approaches for NGS data first map the reads to the genome and then analyze the data based on the mapped reads. Since many organisms have unknown genome sequences and many reads cannot be uniquely mapped to the genomes even if the genome sequences are known, alternative analytical methods are needed for the study of NGS data. Here we suggest using word patterns to analyze NGS data. Word pattern counting (the study of the probabilistic distribution of the number of occurrences of word patterns in one or multiple long sequences) has played an important role in molecular sequence analysis. However, no studies are available on the distribution of the number of occurrences of word patterns in NGS reads. In this article, we build probabilistic models for the background sequence and the sampling process of the sequence reads from the genome. Based on the models, we provide normal and compound Poisson approximations for the number of occurrences of word patterns from the sequence reads, with bounds on the approximation error. The main challenge is to consider the randomness in generating the long background sequence, as well as in the sampling of the reads using NGS. We show the accuracy of these approximations under a variety of conditions for different patterns with various characteristics. Under realistic assumptions, the compound Poisson approximation seems to outperform the normal approximation in most situations. These approximate distributions can be used to evaluate the statistical significance of the occurrence of patterns from NGS data. The theory and the computational algorithm for calculating the approximate distributions are then used to analyze ChIP-Seq data using transcription factor GABP. Software is available online (www-rcf.usc.edu/∼fsun/Programs/NGS_motif_power/NGS

  5. Annual report of the Public Health Authority of the Slovak Republic in 2005

    International Nuclear Information System (INIS)

    A brief account of activities carried out by the Public Health Authority of the Slovak Republic (UVZ SR) in 2005 is presented. These activities are reported under the headings: (1) Structural organization of the UVZ SR; (2) Section Hygiene Living and Working Conditions; (3) Division of Health Protection Against Radiation; (4) Epidemiology Section; (5) Division of Medical Microbiology; (6) Section Protection, Promotion and Development of Health; (7) Department of objectification factors welfare.

  6. Annual activity report of the Public Health Authority of the Slovak Republic for 2007

    International Nuclear Information System (INIS)

    A brief account of activities carried out by the Public Health Authority of the Slovak Republic (UVZ SR) in 2007 of regional offices is presented. These activities are reported under the headings: (1) Environment; (2) Preventive occupational medicine; (3) Food Hygiene; (4) Hygiene of children and youth; (5) Epidemiology; (6) Laboratories objectification factors and working environments; (7)Medical microbiology; (8) Health education; (9) Health protection against radiation; (10) Complaints and petitions; (11) Evaluation of punitive measures for 2007.

  7. Thermal performance monitoring at Pickering NGS using THERMAC

    International Nuclear Information System (INIS)

    A strategy has been implemented at Pickering Nuclear Generating Station to monitor the thermal performance of the turbine cycle. A key element of this strategy has been the implementation of the generic power plant performance monitoring tool THERMAC. With the help of THERMAC analyses, station personal can diagnose and locate thermal losses, optimize maintenance frequency, and ultimately, increase total station electrical output. This paper provides an introduction to the THERMAC software. The structure and layout of the program, as seen by the user, will be discussed. The paper will focus on the custom implementation of THERMAC at Pickering NGS. An example of a THERMAC analysis that demonstrates the power and usefulness of the software will be presented. Other aspects of the thermal performance monitoring strategy, such as heat rate testing and instrumentation improvements, will also be discussed. 1 ref., 2 figs

  8. IPv6与下一代服务(NGS)

    Institute of Scientific and Technical Information of China (English)

    雷振洲

    2005-01-01

    大家知道现在我们都在走PSTN电信网络NGN,下一代Internet就是NGI,移动网要走向3G/B3G.那么他们为什么要走向NGS,实际上特别是上个世纪90年代的移动通信告诉我们现在的网络不能适应将来的要求,为什么?互联网的大发展说明人对信息的需求、对数据的需求、对未来更有价值的服务应用的需求越来越高.移动的大发展说明移动性对个性化对无所不在的需求是越来越强烈了.

  9. Annual activity report of the Public Health Authority of the Slovak Republic for 2009

    International Nuclear Information System (INIS)

    A brief account of activities carried out by the Public Health Authority of the Slovak Republic (UVZ SR) in 2009 is presented. These activities are reported under the headings: (1) General section; (2) Department of legislation and law; (3) Department of control, surveillance and complaints; (4) Department of environmental health; (5) Department of preventive occupational medicine; (6) Department of food hygiene, food safety and cosmetic products; (7) Department of hygiene of children and youth; (8) Department of objectification factors welfare; (9) Department of medical microbiology; (10) Department of epidemiology; (11) Department of health protection against radiation; (12) Department of health promotion; (13) Department of alcohol and tobacco control.

  10. Annual report of the Public Health Authority of the Slovak Republic for 2007

    International Nuclear Information System (INIS)

    A brief account of activities carried out by the Public Health Authority of the Slovak Republic (UVZ SR) in 2006 is presented. These activities are reported under the headings: (1) General section; (2) Department of legislation and law; (3) Department of control, surveillance and complaints; (4) Department of environmental health; (5) Department of preventive occupational medicine; (6) Department of food hygiene, food safety and cosmetic products; (7) Department of hygiene of children and youth; (8) Department of objectification factors welfare; (9) Department of medical microbiology; (10) Department of epidemiology; (11) Department of health protection against radiation; (12) Department of health promotion; (13) Center of tobacco control.

  11. Annual activity report of the Public Health Authority of the Slovak Republic for 2008

    International Nuclear Information System (INIS)

    A brief account of activities carried out by the Public Health Authority of the Slovak Republic (UVZ SR) in 2008 is presented. These activities are reported under the headings: (1) General section; (2) Department of legislation and law; (3) Department of control, surveillance and complaints; (4) Department of environmental health; (5) Department of preventive occupational medicine; (6) Department of food hygiene, food safety and cosmetic products; (7) Department of hygiene of children and youth; (8) Department of objectification factors welfare; (9) Department of medical microbiology; (10) Department of epidemiology; (11) Department of health protection against radiation; (12) Department of health promotion; (13) Department of alcohol and tobacco control.

  12. Annual activity report of the Public Health Authority of the Slovak Republic for 2006

    International Nuclear Information System (INIS)

    A brief account of activities carried out by the Public Health Authority of the Slovak Republic (UVZ SR) in 2006 is presented. These activities are reported under the headings: (1) General section; (2) Department of legislation and law; (3) Department of control, surveillance and complaints; (4) Department of environmental health; (5) Department of preventive occupational medicine; (6) Department of food hygiene, food safety and cosmetic products; (7) Department of hygiene of children and youth; (8) Department of objectification factors welfare; (9) Department of medical microbiology; (10) Department of epidemiology; (11) Department of health protection against radiation; (12) Department of health promotion; (13) Center of tobacco control; (14) Crisis management unit.

  13. Annual report on activities of Regional Public Health Offices in the Slovak Republic in 2008

    International Nuclear Information System (INIS)

    A brief account of activities carried out by the Regional Public Health Offices in the Slovak Republic in 2008 is presented. These activities are reported under the headings: (1) Environment; (2) Preventive occupational medicine; (3) Hygiene, nutrition, food safety and cosmetic products; (4) Hygiene of children and youth; (5) Epidemiology; (6) Objectification of environmental factors and working environment; (7) Medical microbiology; (8) Health promotion; (9) Health protection against radiation; (10) Complaints and petitions; (11) Control of tobacco and alcohol; (12) Evaluation of punitive measures for 2008.

  14. Annual report of the Public Health Authority of the Slovak Republic for 2011

    International Nuclear Information System (INIS)

    A brief account of activities carried out by the Public Health Authority of the Slovak Republic (UVZ SR) in 2011 is presented. These activities are reported under the headings: (1) General part; (2) Legislation section; (3) Section of control, supervision and complaints; (4) Section of environmental hygiene; (5) Section hygiene living and working conditions; (6) Department of food hygiene, food safety and cosmetic products; (7) Department of hygiene of children and youth; (8) Department of objectification factors welfare; (9) Division of Medical Microbiology; (10) Epidemiology Section; (11) Division of health protection against radiation.

  15. Annual report of the Public Health Authority of the Slovak Republic for 2010

    International Nuclear Information System (INIS)

    A brief account of activities carried out by the Public Health Authority of the Slovak Republic (UVZ SR) in 2010 is presented. These activities are reported under the headings: (1) General pat; (2) Legislation section; Section of control, supervision and complaints; (3) Section of environmental hygiene; (4) Section hygiene living and working conditions; (5) Department of food hygiene, food safety and cosmetic products; (6) Department of hygiene of children and youth; (7) Department of objectification factors welfare; (8) Division of medical microbiology; (9) Epidemiology section; (10) Division of health protection against radiation; (11) Department of health promotion; (12) Department of alcohol and tobacco Control.

  16. Electric and Magnetic Fields Research and Public Information Dissemination Program annual report for fiscal year 1996

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-06-01

    The Electric and Magnetic Fields (EMF) Research and Public Information Dissemination (RAPID) Program was authorized by the Energy Policy Act of 1992 as a near-term effort to expand and accelerate the research needed to address the EMF issue. As required by this legislation, the EMF Interagency Committee, the National EMF Advisory Committee (NEMFAC), and the National Academy of Sciences (NAS) are providing valued input and advice for the direction of this program. With this input and advice, the Department of Energy (DOE) and the National Institute of Environmental Health Sciences (NIEHS) have developed and are implementing five-year program plans. Multi-year health effects research projects and related EMF measurement and exposure assessment projects are underway using funds appropriated in fiscal years 1994, 1995, and 1996 together with voluntary non-Federal contributions. The results of these research projects, along with the results of other EMF research, will be used as input to the hazard evaluation effort, which is the focus of the EMF RAPID Program. A coordinated interagency program is underway to communicate needed information on the EMF issue in a clear manner to the public and other decision makers.

  17. Energy and climate change: the main analyses of Regards sur la Terre. An annual publication on sustainable development

    International Nuclear Information System (INIS)

    In November 2006, the French Development Agency, AFD (Agence francaise de developpement) and the Institute for Sustainable Development and International Relations, IDDRI (Institut du developpement durable et des relations internationales) launched an annual publication on sustainable development in a global perspective, Regards sur la Terre, published by Les Presses de Sciences Po (Paris). Regards sur la Terre includes an analysis of the most important international events of the last twelve months in the field of sustainable development, along with a thematic section, which in the first edition focused on energy and climate change. This booklet presents the overall introduction of the 2007 publication and the introduction of its thematic section, as well as a selection of the main chapters dealing with the theme of energy and climate change. Contents: Awakening and crisis of confidence; Reorienting our Societies; Energy in the world: Challenges and prospects; Challenges and constraints for energy supply: The coal hard facts; Satisfying energy growth in emerging countries; Diversifying power generation in China; From Rio to Marrakech: Development in climate negotiations; An international coordination regime come what may; The perspective of developing countries; An American 'point of view'

  18. Publication rates of manuscript presentations at the American College of Foot and Ankle Surgeons Annual Scientific Conference between 1999 and 2008.

    Science.gov (United States)

    Roukis, Thomas S

    2011-01-01

    Publication is the ultimate desired end point of scientific research. However, oral manuscript presentations of research studies are often referenced in textbooks, journal articles, and industry white papers, and, as a result, influence treatment care plans. No data exist for the actual publication rate of podiatric foot and ankle surgery oral manuscript presentations. Therefore, the objective of this study was to determine the actual publication rates of oral manuscript presentations at the American College of Foot and Ankle Surgeons (ACFAS) Annual Scientific Conference over 10 years. Print or electronic media for the ACFAS Annual Scientific Conference official program between 1999 and 2008 were obtained. Each year's official program was hand searched for any oral manuscript presentation, and, when identified, the title and authors were individually searched through electronic internet-based search engines to determine whether an oral manuscript presentation had been followed by publication of a full-text article. Additionally, pertinent journals were hand searched for potential articles. A total of 67.5% (139/206) oral manuscript presentations were ultimately published in 1 of 12 medical journals in a mean of 14.5 months. All journals except one (91.7%) represented peer-reviewed journals. The publication rate of oral manuscript presentations at the ACFAS Annual Scientific Conference is similar to or greater than orthopaedic subspecialties, including foot and ankle surgery, publication rates. Based on the above, attendees of the ACFAS Annual Scientific Conference should be aware that the majority of oral manuscript material presented at the ACFAS Annual Scientific Conference can be considered as accurate because they survive the rigors of the peer-review process more than two thirds of the time.

  19. Publication and dissemination of Annual Reports to shareholders in Nigeria and the United Kingdom: The Economics of Social Media and Electronic Communication

    Directory of Open Access Journals (Sweden)

    Olagoke Kuye

    2015-06-01

    Full Text Available While the cost of printing and sending out annual reports to shareholders by companies keep increasing and arguments have been made for cheaper and cost-effective ways of carrying out financial disclosure obligations by companies including electronic method, Companies’ Acts maintain that shareholders remain the ultimate decision-maker in the question whether electronic means of publishing annual reports is to be adopted as the primary means of publishing and disseminating annual reports among shareholders. This line of reasoning by legislation is given impetus by the staple assumption that shareholders take the form of owners of the companies of which they are members and the question of what mode of publication and dissemination of annual reports, whether electronic or hard copy remains their exclusive preserve. However, a case is made for a more cost-effective means of carrying out companies’ disclosure obligation through the vehicle of social media and electronic means.

  20. 4. Annual meeting of the Society of Hygiene, Environment and Public Health Sciences. Abstracts; 4. Jahrestagung der Gesellschaft fuer Hygiene, Umweltmedizin und Praeventivmedizin (GHUP). Zusammenfassungen

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2010-07-01

    The volume contains the abstracts of the 4th annual meeting of the Society of Hygiene, Environment and Public Health Sciences. The contributions cover the following issues: hygiene, environment and preventive medicine; children' health and prevention; climatic change and health; human biomonitoring; mold fungus and bioaerosols; local and supraaregional infection prevention; free topics: water, land air.

  1. Public Utility Regulatory Policies Act of 1978. Annual report to Congress

    Energy Technology Data Exchange (ETDEWEB)

    None,

    1980-05-01

    Titles I and III of the Public Utility Regulatory Policies Act of 1978 (PURPA) establish retail regulatory policies for electric and natural gas utilities, respectively, aimed at achieving three purposes: conservation of energy supplied by electric and gas utilities; efficiency in the use of facilities and resources by these utilities; equitable rates to electricity and natural gas consumers. PURPA also continues the pilot utility implementation program, authorized under Title II of the Energy Conservation and Production ACT (ECPA), to encourage adoption of cost-based rates and efficient energy-management practices. The purpose of this report is twofold: (1) to summarize and analyze the progress that state regulatory authorities and certain nonregulated utilities have made in their consideration of the PURPA standards; and (2) to summarize the Department of Energy (DOE) activities relating to PURPA and ECPA. The report provides a broad overview and assessment of the status of electric and gas regulation nationwide, and thus helps provide the basis for congressional and DOE actions targeted on the utility industry to address pressing national energy problems.

  2. The NGS WikiBook: a dynamic collaborative online training effort with long-term sustainability.

    Science.gov (United States)

    Li, Jing-Woei; Bolser, Dan; Manske, Magnus; Giorgi, Federico Manuel; Vyahhi, Nikolay; Usadel, Björn; Clavijo, Bernardo J; Chan, Ting-Fung; Wong, Nathalie; Zerbino, Daniel; Schneider, Maria Victoria

    2013-09-01

    Next-generation sequencing (NGS) is increasingly being adopted as the backbone of biomedical research. With the commercialization of various affordable desktop sequencers, NGS will be reached by increasing numbers of cellular and molecular biologists, necessitating community consensus on bioinformatics protocols to tackle the exponential increase in quantity of sequence data. The current resources for NGS informatics are extremely fragmented. Finding a centralized synthesis is difficult. A multitude of tools exist for NGS data analysis; however, none of these satisfies all possible uses and needs. This gap in functionality could be filled by integrating different methods in customized pipelines, an approach helped by the open-source nature of many NGS programmes. Drawing from community spirit and with the use of the Wikipedia framework, we have initiated a collaborative NGS resource: The NGS WikiBook. We have collected a sufficient amount of text to incentivize a broader community to contribute to it. Users can search, browse, edit and create new content, so as to facilitate self-learning and feedback to the community. The overall structure and style for this dynamic material is designed for the bench biologists and non-bioinformaticians. The flexibility of online material allows the readers to ignore details in a first read, yet have immediate access to the information they need. Each chapter comes with practical exercises so readers may familiarize themselves with each step. The NGS WikiBook aims to create a collective laboratory book and protocol that explains the key concepts and describes best practices in this fast-evolving field.

  3. Targeted NGS meets expert clinical characterization: Efficient diagnosis of spastic paraplegia type 11

    OpenAIRE

    Cristina Castro-Fernández; Manuel Arias; Patricia Blanco-Arias; Luis Santomé-Collazo; Jorge Amigo; Ángel Carracedo; Maria-Jesús Sobrido

    2015-01-01

    Next generation sequencing (NGS) is transforming the diagnostic approach for neurological disorders, since it allows simultaneous analysis of hundreds of genes, even based on just a broad, syndromic patient categorization. However, such an approach bears a high risk of incidental and uncertain genetic findings. We report a patient with spastic paraplegia whose comprehensive neurological and imaging examination raised a high clinical suspicion of SPG11. Thus, although our NGS pipeline for this...

  4. NGS QC Toolkit: A Toolkit for Quality Control of Next Generation Sequencing Data

    OpenAIRE

    Patel, Ravi K.; Mukesh Jain

    2012-01-01

    Next generation sequencing (NGS) technologies provide a high-throughput means to generate large amount of sequence data. However, quality control (QC) of sequence data generated from these technologies is extremely important for meaningful downstream analysis. Further, highly efficient and fast processing tools are required to handle the large volume of datasets. Here, we have developed an application, NGS QC Toolkit, for quality check and filtering of high-quality data. This toolkit is a sta...

  5. Targeted NGS: a cost effective approach to molecular diagnosis of PIDs

    OpenAIRE

    Jennifer eStoddard; Julie eNiemela; Thomas Arthur Fleisher; Sergio eRosenzweig

    2014-01-01

    Background: Primary immunodeficiencies (PIDs) are a diverse group of disorders caused by multiple genetic defects. Obtaining a molecular diagnosis for PID patients using a phenotype-based approach is often complex, expensive, and not always successful. Next-generation sequencing (NGS) methods offer an unbiased genotype-based approach, which can facilitate molecular diagnostics.Objective: To develop an efficient NGS method to identify variants in PID-related genes.Methods: We performed HaloPle...

  6. ngs_backbone: a pipeline for read cleaning, mapping and SNP calling using Next Generation Sequence

    OpenAIRE

    Cañizares Joaquin; Nuez Fernando; Ziarsolo Peio; Pascual Laura; Blanca Jose M

    2011-01-01

    Background: The possibilities offered by next generation sequencing (NGS) platforms are revolutionizing biotechnological laboratories. Moreover, the combination of NGS sequencing and affordable high-throughput genotyping technologies is facilitating the rapid discovery and use of SNPs in non-model species. However, this abundance of sequences and polymorphisms creates new software needs. To fulfill these needs, we have developed a powerful, yet easy-to-use application. Results:...

  7. Chattering of bleed condenser relief valves in Darlington NGS

    International Nuclear Information System (INIS)

    The Bleed Condenser (BC) is part of the Pressure and Inventory Control System. The Primary Heat Transport System (PHTS) will transfer water to the Bleed Condenser via the PHT liquid relief valves (LRV) every time overpressure occurs in the main circuit. The BC is designed to withstand full PHT pressure and is protected against overpressurization by two spring loaded Relief Valves (RV). Chattering of these relief valves was observed during commissioning tests in Darlington, when opening of the LRVs pressurized the bleed condenser above the RVs set point. The chattering caused a drift in the valve setpoint and ruptured a pipe connected to the relief line. Similar undesirable events were observed in Bruce NGS. This paper presents the thermalhydraulic analysis of the system, during the above mentioned event and proposes several possible solutions to the problem. The computer simulation was performed using the SOPHT code, developed by Ontario Hydro. Considering the various limitations imposed by design on the relief valves and on the system, the recommended solution is to prolong the valve closing time by 400-800 milliseconds. (author) 9 refs., 11 figs

  8. Pickering NGS 'A' Unit 3 retube tooling quality review

    International Nuclear Information System (INIS)

    A review of tooling quality related issues for the retubing of the Pickering NGS 'A' Unit 3 CANDU reactor was performed with the intention of using the experience to benefit future retube programs. The review used Retube Deficiency Reports (RDR), and other supplemental records to assemble information on the reasons, types, timing and severity of the modifications required to correct a tool problem. It also used results of a separate Retubing Downtime study to correlate the tooling records to downtime. The aim of the review is to identify areas of deficiencies in the tooling development process so that resources can be devoted to those areas and thus eliminate problems before going to the reactor face. Overall, the tools performed their basic functions well, but often lacked attention to detail in areas such as; mechanical, electrical, ergonomics, decontamination, maintenance, material handling and visual aids. These deficiencies as well as a relatively small number of major problems resulted in significant amounts of downtime and expenditures of resources at site. Several recommendations arose from the study which include: more emphasis on correcting deficiencies at the design organization, before shipping the tool to site through improved mockups, design guidelines and more emphasis on detailed design reviews; improved mockups on site and more thorough tool proving using both experienced and trainee operators; and, improvements in documentation of design requirements for the tools at the design organization and of tool problems at site

  9. Using REDItools to Detect RNA Editing Events in NGS Datasets.

    Science.gov (United States)

    Picardi, Ernesto; D'Erchia, Anna Maria; Montalvo, Antonio; Pesole, Graziano

    2015-03-09

    RNA editing is a post-transcriptional/co-transcriptional molecular phenomenon whereby a genetic message is modified from the corresponding DNA template by means of substitutions, insertions, and/or deletions. It occurs in a variety of organisms and different cellular locations through evolutionally and biochemically unrelated proteins. RNA editing has a plethora of biological effects including the modulation of alternative splicing and fine-tuning of gene expression. RNA editing events by base substitutions can be detected on a genomic scale by NGS technologies through the REDItools package, an ad hoc suite of Python scripts to study RNA editing using RNA-Seq and DNA-Seq data or RNA-Seq data alone. REDItools implement effective filters to minimize biases due to sequencing errors, mapping errors, and SNPs. The package is freely available at Google Code repository (http://code.google.com/p/reditools/) and released under the MIT license. In the present unit we show three basic protocols corresponding to three main REDItools scripts.

  10. Relaxation of containment: Pickering NGS-A unit 3 rehabilitation outage

    International Nuclear Information System (INIS)

    A reactor containment system provides designed barriers between radioactive sources in reactor systems and the general public. Normally, access to the reactor systems is controlled via airlocks in the Reactor Building so that the containment system integrity can be maintained unimpaired. During reactor retubing, however, a more direct access to the reactor is necessary in order that retubing operations can be conducted efficiently. The Pickering NGS containment system is described briefly together with the detailed strategy adopted to relax the guidelines governing containment, which permits a less restrictive access to the reactor. Topics discussed include prerequisite work, the control strategy and procedures employed during relaxation as well as supporting analyses. Of particular importance is the need to maintain quality and operational standards, where required, so that the containment envelope can be reestablished quickly when and if required. This paper will be of interest to all CANDU owner/operators regardless of containment design and is potentially of interest to operators of other containment designs. It is anticipated that future containment relaxations at other CANDU sites in Canada will follow this strategy as a baseline. (author). 3 tabs., 5 figs

  11. NGS-based identification of druggable alterations and signaling pathways – hepatocellular carcinoma case report

    Directory of Open Access Journals (Sweden)

    Kotelnikova E. A.

    2015-12-01

    Full Text Available Aim. To identify potential cancer driving or clinically relevant molecular events for a patient with hepatocellular carcinoma. Methods. In order to achieve this goal, we performed RNA-seq and exome sequencing for the tumor tissue and its matched control. We annotated the alterations found using several publicly available databases and bioinformatics tools. Results. We identified several differentially expressed genes linked to the classical sorafenib treatment as well as additional pathways potentially druggable by therapies studied in clinical trials (Erlotinib, Lapatinib and Temsirolimus. Several germline mutations, found in XRCC1, TP53 and DPYD, according to the data from other clinical trials, could be related to the increased sensitivity to platinum therapies and reduced sensitivity to 5-Fluorouracil. We also identified several potentially driving mutations that could not be currently linked to therapies, like deletion in CIRBP, SNVs in BTG1, ERBB3, TCF7L2 et al. Conclusions. The presented study shows the potential usefulness of the integrated approach to the NGS data analysis, including the analysis of germline mutations and transcriptome in addition to the cancer panel or the exome sequencing data.

  12. 78 FR 48421 - Publication of North American Datum of 1983 (2011) Epoch 2010.00, North American Datum of 1983...

    Science.gov (United States)

    2013-08-08

    ..., 54 FR 25318, June 14, 1989). DATES: Individuals or organizations wishing to submit comments on the...) Epoch 2010.00 AGENCY: National Geodetic Survey (NGS), National Ocean Service (NOS), National Oceanic and... Geodetic Survey (NGS) has finalized the publication of new realizations of three geodetic references...

  13. Targeted NGS: a cost effective approach to molecular diagnosis of PIDs

    Directory of Open Access Journals (Sweden)

    Jennifer eStoddard

    2014-11-01

    Full Text Available Background: Primary immunodeficiencies (PIDs are a diverse group of disorders caused by multiple genetic defects. Obtaining a molecular diagnosis for PID patients using a phenotype-based approach is often complex, expensive, and not always successful. Next-generation sequencing (NGS methods offer an unbiased genotype-based approach, which can facilitate molecular diagnostics.Objective: To develop an efficient NGS method to identify variants in PID-related genes.Methods: We performed HaloPlex custom target enrichment and NGS using the Ion Torrent PGM to screen 173 genes in 11 healthy controls, 13 PID patients previously evaluated with either an identified mutation or SNP, and 120 patients with undiagnosed PIDs. Sensitivity and specificity were determined by comparing NGS and Sanger sequencing results for 33 patients. Run metrics and coverage analyses were done to identify systematic deficiencies. Results: A molecular diagnosis was identified for 18 of 120 patients who previously lacked a genetic diagnosis, including 9 who had atypical presentations and extensive previous genetic and functional studies. Our NGS method detected variants with 98.1% sensitivity and >99.9% specificity. Uniformity was variable (72-89% and we were not able to reliably sequence 45 regions (45/2455 or 1.8% of total regions due to low (<20 average read depth or <90% region coverage; thus, we optimized probe hybridization conditions to improve read-depth and coverage for future analyses, and established criteria to help identify true-positives. Conclusion: While NGS methods are not as sensitive as Sanger sequencing for individual genes, targeted NGS is a cost-effective, first-line genetic test for the evaluation of patients with PIDs. This approach decreases time to diagnosis, increases diagnostic rate, and provides insight into the genotype-phenotype correlation of PIDs in a cost-effective way.

  14. Next generation sequencing (NGS) database for tandem repeats with multiple pattern 2°-shaft multicore string matching

    OpenAIRE

    Chinta Someswara Rao; Viswanadha Raju, S.

    2016-01-01

    Next generation sequencing (NGS) technologies have been rapidly applied in biomedical and biological research in recent years. To provide the comprehensive NGS resource for the research, in this paper , we have considered 10 loci/codi/repeats TAGA, TCAT, GAAT, AGAT, AGAA, GATA, TATC, CTTT, TCTG and TCTA. Then we developed the NGS Tandem Repeat Database (TandemRepeatDB) for all the chromosomes of Homo sapiens, Callithrix jacchus, Chlorocebus sabaeus, Gorilla gorilla, Macaca fascicularis, Macac...

  15. AECB staff annual report of Darlington NGS 'A' for the year 1990

    International Nuclear Information System (INIS)

    This report presents the AECB staff review of major licensing issues and of the operational performance of the station during 1990. In addition to the reports mentioned above, other Ontario Hydro reports, official correspondence, and observations of AECB site staff have been taken into consideration. The report is limited to those aspects of station performance that AECB staff consider to have some safety significance. Developments of significance associated with issues addressed in the report that occurred in the early part of 1991 are also mentioned

  16. AECB staff annual report of Darlington NGS for the year 1991

    International Nuclear Information System (INIS)

    Ontario Hydro operated Darlington in a safe manner in 1991. Ontario Hydro violated the Atomic Energy Control Regulations once and the physical security regulations three times in 1991. They failed to observe the Operating Licence conditions on ten occasions. The AECB did not find that the individual events had a significant impact on safety. There were no violations of the construction licence. None of the station staff received a radiation dose in excess of the regulatory limit. Radioactive emissions from the station were far below the regulatory limit. Special safety system performance was not fully satisfactory. Ontario Hydro failed to meet the unavailability targets for shutdown system one and the negative pressure containment system. Ontario Hydro reported seventeen incidents under conditions of the Operating and Construction licences. Units 1 and 2 remained shut down for most of 1991 because of unexplained fuel bundle damage in the reactor core. Ontario Hydro has decided to replace the main generator rotors because of cracks discovered on the rotor shaft. A fully modified rotor was installed on Unit 1. Ontario Hydro staff have a significant backlog of maintenance work. The Quality Improvement Program seemed to work well, resulting in some noticeable improvements. Three Shift Supervisors and four Control Operators were licensed this year. All planned emergency exercises and drills took place as scheduled. Ontario Hydro identified and are addressing several areas for improvement during the drills. Except for a power supply interruption to some IAEA equipment, Ontario Hydro achieved all its safeguards goals at Darlington in 1991. The Tritium Removal Facility (TRF) operated intermittently during 1991. Ontario Hydro is proceeding with the design and planning of an annex to the TRF to replace the present temporary facilities. (Author)

  17. Fuel management studies for the Pickering NGS B reactor with reduced adjuster loading

    International Nuclear Information System (INIS)

    The adjuster rods in Pickering NGS B provide reactivity for xenon override, and allow operation for limited time when fuelling is unavailable. The adjusters also help to shape the power distribution. However, the adjuster reactivity load produces a significant burnup penalty. In Pickering, the saving in replacement energy cost from having sufficient xenon override capability to recover from a reactor trip is smaller than the additional fuelling cost caused by the adjuster burnup penalty. This paper discusses various methods of operating the Pickering NGS B reactors with a reduced adjuster loading. This study concluded that operating Pickering NGS B with only five of the original 21 adjusters in the core, in conjunction with a four-and eight-bundle shift fuelling scheme is the best option

  18. Targeted NGS meets expert clinical characterization: Efficient diagnosis of spastic paraplegia type 11.

    Science.gov (United States)

    Castro-Fernández, Cristina; Arias, Manuel; Blanco-Arias, Patricia; Santomé-Collazo, Luis; Amigo, Jorge; Carracedo, Ángel; Sobrido, Maria-Jesús

    2015-06-01

    Next generation sequencing (NGS) is transforming the diagnostic approach for neurological disorders, since it allows simultaneous analysis of hundreds of genes, even based on just a broad, syndromic patient categorization. However, such an approach bears a high risk of incidental and uncertain genetic findings. We report a patient with spastic paraplegia whose comprehensive neurological and imaging examination raised a high clinical suspicion of SPG11. Thus, although our NGS pipeline for this group of disorders includes gene panel and exome sequencing, in this sample only the spatacsin gene region was captured and subsequently searched for mutations. Two probably pathogenic variants were quickly and clearly identified, confirming the diagnosis of SPG11. This case illustrates how combination of expert clinical characterization with highly oriented NGS protocols leads to a fast, cost-efficient diagnosis, minimizing the risk of findings with unclear significance.

  19. NGS-Trex: an automatic analysis workflow for RNA-Seq data.

    Science.gov (United States)

    Boria, Ilenia; Boatti, Lara; Saggese, Igor; Mignone, Flavio

    2015-01-01

    RNA-Seq technology allows the rapid analysis of whole transcriptomes taking advantage of next-generation sequencing platforms. Moreover with the constant decrease of the cost of NGS analysis RNA-Seq is becoming very popular and widespread. Unfortunately data analysis is quite demanding in terms of bioinformatic skills and infrastructures required, thus limiting the potential users of this method. Here we describe the complete analysis of sample data from raw sequences to data mining of results by using NGS-Trex platform, a low user interaction, fully automatic analysis workflow. Used through a web interface, NGS-Trex processes data and profiles the transcriptome of the samples identifying expressed genes, transcripts, and new and known splice variants. It also detects differentially expressed genes and transcripts across different experiments.

  20. Targeted NGS meets expert clinical characterization: Efficient diagnosis of spastic paraplegia type 11

    Directory of Open Access Journals (Sweden)

    Cristina Castro-Fernández

    2015-06-01

    Full Text Available Next generation sequencing (NGS is transforming the diagnostic approach for neurological disorders, since it allows simultaneous analysis of hundreds of genes, even based on just a broad, syndromic patient categorization. However, such an approach bears a high risk of incidental and uncertain genetic findings. We report a patient with spastic paraplegia whose comprehensive neurological and imaging examination raised a high clinical suspicion of SPG11. Thus, although our NGS pipeline for this group of disorders includes gene panel and exome sequencing, in this sample only the spatacsin gene region was captured and subsequently searched for mutations. Two probably pathogenic variants were quickly and clearly identified, confirming the diagnosis of SPG11. This case illustrates how combination of expert clinical characterization with highly oriented NGS protocols leads to a fast, cost-efficient diagnosis, minimizing the risk of findings with unclear significance.

  1. Effects of high and low morpholine operation on corrosion product transport at Bruce NGS A

    International Nuclear Information System (INIS)

    Corrosion product transport studies were carried out on the condensate and feedwater systems for two of the units at Bruce Nuclear Generating Station-A (Bruce NGS-A). Bruce NGS-A is a four unit station with a mixed copper/iron feedtrain and operates under morpholine chemistry. During the studies, two different morpholine chemistries were evaluated. When high morpholine concentrations were used, lower iron transport was reported; but this was offset by increased copper and zinc transport. The distribution of morpholine and its degradation products throughout the secondary system is also discussed. (author)

  2. Genetic Architecture of Milk, Fat, Protein, Mastitis and Fertility Studied using NGS Data in Holstein Cattle

    DEFF Research Database (Denmark)

    Sahana, Goutam; Janss, Luc; Guldbrandtsen, Bernt;

    cattle using NGS variants. The analysis was done using a linear mixed model (LMM) and a Bayesian mixture model (BMM). The top 10 QTL identified by LMM analyses explained 22.61, 23.86, 10.88, 18.58 and 14.83% of the total genetic variance for these traits respectively. Trait-specific sets of 4,964 SNPs...... from NGS variants (most ‘associated’ SNP for each 0.5 Mbp bin) explained 81.0, 81.6, 85.0, 60.4 and 70.9% of total genetic variance for milk, fat, protein, mastitis and fertility indices when analyzed simultaneously by BMM...

  3. Quality Control and Pre-Qualification of NGS Libraries Made from Clinical Samples

    OpenAIRE

    Langmore, John

    2013-01-01

    We have compared sequencing metrics from different types of clinical samples and different methods of making NGS libraries, for purposes of quality control of the samples and sample preps. We have performed the metrics using the HiSeq and MiSeq, however the same QC metrics could be measured on other platforms. By choosing metrics that can be measured from small amounts of data (e.g., 300,000 reads), we can measure the quality of the clinical samples and NGS libraries in a highly multiplexed f...

  4. A Web-Hosted R Workflow to Simplify and Automate the Analysis of 16S NGS Data

    Science.gov (United States)

    Next-Generation Sequencing (NGS) produces large data sets that include tens-of-thousands of sequence reads per sample. For analysis of bacterial diversity, 16S NGS sequences are typically analyzed in a workflow that containing best-of-breed bioinformatics packages that may levera...

  5. ViennaNGS: A toolbox for building efficient next- generation sequencing analysis pipelines [v2; ref status: indexed, http://f1000r.es/5mq

    OpenAIRE

    Wolfinger, Michael T.; Jörg Fallmann; Florian Eggenhofer; Fabian Amman

    2015-01-01

    Recent achievements in next-generation sequencing (NGS) technologies lead to a high demand for reuseable software components to easily compile customized analysis workflows for big genomics data. We present ViennaNGS, an integrated collection of Perl modules focused on building efficient pipelines for NGS data processing. It comes with functionality for extracting and converting features from common NGS file formats, computation and evaluation of read mapping statistics, as well as normalizat...

  6. Environmental Sciences Division annual progress report for period ending September 30, 1982. Environmental Sciences Division Publication No. 2090. [Lead abstract

    Energy Technology Data Exchange (ETDEWEB)

    1983-04-01

    Separate abstracts were prepared for 12 of the 14 sections of the Environmental Sciences Division annual progress report. The other 2 sections deal with educational activities. The programs discussed deal with advanced fuel energy, toxic substances, environmental impacts of various energy technologies, biomass, low-level radioactive waste management, the global carbon cycle, and aquatic and terrestrial ecology. (KRM)

  7. Environmental Sciences Division annual progress report for period ending September 30, 1982. Environmental Sciences Division Publication No. 2090

    International Nuclear Information System (INIS)

    Separate abstracts were prepared for 12 of the 14 sections of the Environmental Sciences Division annual progress report. The other 2 sections deal with educational activities. The programs discussed deal with advanced fuel energy, toxic substances, environmental impacts of various energy technologies, biomass, low-level radioactive waste management, the global carbon cycle, and aquatic and terrestrial ecology

  8. Scientific annual report 1980

    International Nuclear Information System (INIS)

    This annual report contains a collection of the abstracts of the publications concerning research and development in the named institute together with a bibliography about further publications, contributions to conferences and speeches. (HSI)

  9. Pickering NGS unit 7 gadolinium oxalate formation event: investigation and recovery

    International Nuclear Information System (INIS)

    In April 2008, an unexpected chemistry event occurred during a guaranteed shutdown state on Pickering NGS unit 7. The event is described here, as well as subsequent investigations and eventual return-to-service of the unit. It is expected that lessons learned from this event are applicable to CANDU reactors in general. (author)

  10. RepARK--de novo creation of repeat libraries from whole-genome NGS reads.

    Science.gov (United States)

    Koch, Philipp; Platzer, Matthias; Downie, Bryan R

    2014-05-01

    Generation of repeat libraries is a critical step for analysis of complex genomes. In the era of next-generation sequencing (NGS), such libraries are usually produced using a whole-genome shotgun (WGS) derived reference sequence whose completeness greatly influences the quality of derived repeat libraries. We describe here a de novo repeat assembly method--RepARK (Repetitive motif detection by Assembly of Repetitive K-mers)--which avoids potential biases by using abundant k-mers of NGS WGS reads without requiring a reference genome. For validation, repeat consensuses derived from simulated and real Drosophila melanogaster NGS WGS reads were compared to repeat libraries generated by four established methods. RepARK is orders of magnitude faster than the other methods and generates libraries that are: (i) composed almost entirely of repetitive motifs, (ii) more comprehensive and (iii) almost completely annotated by TEclass. Additionally, we show that the RepARK method is applicable to complex genomes like human and can even serve as a diagnostic tool to identify repetitive sequences contaminating NGS datasets.

  11. Environmental Performance Report 2013: Annual Site Environmental Report per the U.S. Department of Energy Order 231.1-1B (Management Publication)

    Energy Technology Data Exchange (ETDEWEB)

    Schlomberg, K.; Eickhoff, J.; Beatty, B.; Braus, G.; Durbin, L.; Fiehweg, R.; Ray, M.; Ryon, T.; Schmitz, E.

    2014-08-01

    The National Renewable Energy Laboratory's (NREL's) Environmental Performance Report provides a description of the laboratory's environmental management activities for 2013, including information on environmental and sustainability performance, environmental compliance activities and status, and environmental protection programs, highlights, and successes. The purpose of this report is to ensure that U.S. Department of Energy (DOE) and the public receive timely, accurate information about events that have affected or could adversely affect the health, safety, and security of the public or workers; the environment; or the operations of DOE facilities. This report meets the requirements of the Annual Site Environmental Report and is prepared in accordance with the DOE Order 231.1B, Environment, Safety and Health Reporting.

  12. An Assessment of Airborn Gravimetry Collected under the NGS GRAV-D Project

    Science.gov (United States)

    Holmes, S. A.

    2009-12-01

    In the United States, the National Geodetic Survey [NGS] holds the official charter to maintain the vertical datum for the USA and its territories. This includes the responsibility to maintain a current geoid model for transforming between orthometric (H) and geodetic (h) heights. The latest (2009) NGS geoid model incorporates the latest GRACE-based satellite-only geopotential solutions, very-high-resolution digital elevation models [DEMs] derived from the Shuttle Radar Topography Mission [SRTM], and other additional data and processing improvements. This recent geoid model also benefited greatly from the prior release of the National Geospatial-Intelligence Agency’s [NGA] Earth Gravitational Model 2008 [EGM2008], which served as a computational reference field for the new geoid, but was also particularly useful for identifying and removing corrupted data from the NGS gravimetry database. Looking forwards, NGS intends to increase its efforts to refine and improve its future national geoid models. To this end, their ambitious "Gravity for the Re-definition of the American Datum” [GRAV-D] project aims to update the NGS gravimetry holdings by flying new airborn gravity surveys over a large fraction of the USA and it territories. Concurrent efforts will focus on developing new processing techniques for optimally incorporating improved gravimetry into the final geoid solution. To this end, the GRAV-D team has already flown several surveys in the Gulf of Mexico, Puerto Rico, US Virgin Islands, and Alaska. Testing and analysis aimed at calibrating and validating the preliminary survey data is already underway. The latest assessment of these recent efforts, including the extent to which this new data can be expected to contribute to an improved gravimetric geoid model, is presented here.

  13. Bayerisches Landesamt fuer Umweltschutz. 1997 annual report and abstracts of publications; Bayerisches Landesamt fuer Umweltschutz. Taetigkeitsbericht 1997 und Kurzbeitraege

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-08-01

    The annual report informs about the activities of the Bavarian Land Office for Environmental Protection in the following fields: clean air policy, waste management, management of soil pollution and remedial action, nuclear energy and radiation protection, nature conservation and landscape conservation, LfU laboratory, environmental hygiene, and data processing (SR) [Deutsch] Der vorliegende Bericht beschreibt die Taetigkeiten des Bayerischen Landesamtes fuer Umweltschutz auf folgenden Gebieten: Immissionsschutz, Abfallwirtschaft, Altlasten und Bodenschutz, Kernenergie und Strahlenschutz, Naturschutz und Landschaftspflege, Zentrallabors und Umwelthygiene, sowie Datenverarbeitung. (SR)

  14. Statement from the 1983 meeting of the International Commission on Radiological Protection: annual limits for intakes (ALI) and derived air concentrations (DAC) for members of the public

    International Nuclear Information System (INIS)

    The limitation of the committed effective dose equivalent for members of the public is sufficient to provide compliance over a lifetime with the limit for single organs, thus avoiding non-stochastic effects. Relative values for infants and adults of the committed dose equivalent in a number of tissues per unit intake for each of a few radionuclides have been given: the values for infants are just more than 1 up to 100 times greater than those for adult workers. In each of these cases the appropriate annual dose-equivalent limits recommended by the Commission for members of the public are 10 times less than the corresponding values for workers; the resulting ALI for infants aged six months will be smaller than the values given in ICRP Publication 30 for limiting stochastic effects in workers by factors that range from just more than 10 (for caesium-137) to 1,000 (for ingested plutonium-239). Intermediate factors would apply for older members of the public. The magnitude of the range emphasises the need to consider each situation carefully, with particular reference to children and women. (author)

  15. Betting on Teachers: The 43rd Annual Phi Delta Kappa/Gallup Poll of the Public's Attitudes toward the Public Schools

    Science.gov (United States)

    Bushaw, William J.; Lopez, Shane J.

    2011-01-01

    This is the latest in a series of polls sponsored by Phi Delta Kappa International with the Gallup organization. Some important findings of this year's poll include: About half of us believe teacher unions are hurting public education, but we're more likely to support teacher union leaders than governors in disputes over teacher collective…

  16. The Tsimane’ Amazonian Panel Study (TAPS): Nine years (2002-2010) of annual data available to the public

    Science.gov (United States)

    Leonard, William R.; Reyes-García, Victoria; Tanner, Susan; Rosinger, Asher; Schultz, Alan; Vadez, Vincent; Zhang, Rebecca; Godoy, Ricardo

    2016-01-01

    This brief communication contains a description of the 2002-2010 annual panel collected by the Tsimane’ Amazonian Panel Study team. The study took place among the Tsimane’, a native Amazonian society of forager-horticulturalists. The team tracked a wide range of socio-economic and anthropometric variables from all residents (633 adults ≥16 years; 820 children) in 13 villages along the Maniqui River, department of Beni. The panel is ideally suited to examine how market exposure and modernization affect the well-being of a highly autarkic population and to examine human growth in a non-Western rural setting. PMID:26280812

  17. Introduction and application of South NGS - 200 type of GPS%南方NGS-2O0型GPS测量系统及其应用

    Institute of Scientific and Technical Information of China (English)

    郭胜利

    2002-01-01

    GPS(Global Positioning System)是全球卫星定位系统的缩写.南方NGS-200型GPS测量系统及其在永定河干堤加固工程中的应用表明,GPS测量相对传统的作业模式具有突破性的改变,这一新技术的应用使得广大测量工作者从繁重的外业劳动中解脱出来,并且工作效率有了根本性的提高.

  18. Improved maintenance procedures at Bruce NGS-B

    International Nuclear Information System (INIS)

    At Bruce and Darlington, the fuel handling systems were supplied by GE Canada, who also supplied manuals for servicing them. At the time of the conference, GE Canada was in the process of installing a new computer publication system, with the capability of electronically creating, editing and revising both text and graphics. This will ensure that the technical people obtain current data from the manuals quickly. Several pages from the manuals are reproduced in this paper

  19. Magnetic Fusion Energy Program. Volume I. Introduction, technical summaries, list of publications, etc., Appendices A-K. Annual report

    International Nuclear Information System (INIS)

    An abstract was prepared for the progress summary on transport theory for open and closed magnetic configurations. Seven abstracts were prepared for included appendices of more detailed work on individual devices. Also included is a list of publications, technical presentations, and DOE program contributions

  20. 26 CFR 301.6104(d)-1 - Public inspection and distribution of applications for tax exemption and annual information...

    Science.gov (United States)

    2010-04-01

    ... 26 Internal Revenue 18 2010-04-01 2010-04-01 false Public inspection and distribution of... Section 301.6104(d)-1 Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF THE TREASURY (CONTINUED... recognition of exemption under section 501, it shall make its application for tax exemption (as defined...

  1. Annulus gas system response tests for Pickering NGS A, unit 1 commissioning

    International Nuclear Information System (INIS)

    This paper reviews the Annulus Gas System response tests carried out during the commissioning of Pickering NGS A, Unit 1 in May/June 1987 at the request of the AECB. It shows that the dynamic responses of the system (the station dew point monitors and moisture beetles) agree with the design predictions for adequate leak detection capability. As a result of the tests a new dew point trending range is recommended in the short term for Pickering NGS and a new hygrometer calibration (D2O in CO2) in place of the present calibration (H2O in N2) is recommended in the long term. This will improve the accuracy of the hygrometers as the calibration environment (D2O in CO2) will match that of the AGS. The beetle blaster lines will be modified to avoid core bypassing and tuning improvements to the dew point model programme will also be implemented

  2. Design of cobalt adjuster rods for new Pickering NGS A core

    International Nuclear Information System (INIS)

    The core of Pickering NGS A was redesigned to acheive a better shutdown system without increasing the number of calandria penetrations. The shutdown system has 73 percent more reactivity than before. The average discharge burnup has been increased by about 5 percent at the expense of some reduced ability to override xenon transients and operation without fuelling. Past experience at Pickering NGS A, has shown that there is less than one forced outage per year per unit where the xenon override time capability is needed. For such a lwo outage frequency, a large xenon override time at the expense of average discharge burnup is not economical. Also, a 36 day of operation without fuelling is quite satisfactory

  3. A case study for cloud based high throughput analysis of NGS data using the globus genomics system

    OpenAIRE

    Krithika Bhuvaneshwar; Dinanath Sulakhe; Robinder Gauba; Alex Rodriguez; Ravi Madduri; Utpal Dave; Lukasz Lacinski; Ian Foster; Yuriy Gusev; Subha Madhavan

    2014-01-01

    Next generation sequencing (NGS) technologies produce massive amounts of data requiring a powerful computational infrastructure, high quality bioinformatics software, and skilled personnel to operate the tools. We present a case study of a practical solution to this data management and analysis challenge that simplifies terabyte scale data handling and provides advanced tools for NGS data analysis. These capabilities are implemented using the “Globus Genomics” system, which is an enhanced Gal...

  4. AnsNGS: An Annotation System to Sequence Variations of Next Generation Sequencing Data for Disease-Related Phenotypes

    OpenAIRE

    Na, Young-Ji; Cho, Yonglae; Kim, Ju Han

    2013-01-01

    Objectives Next-generation sequencing (NGS) data in the identification of disease-causing genes provides a promising opportunity in the diagnosis of disease. Beyond the previous efforts for NGS data alignment, variant detection, and visualization, developing a comprehensive annotation system supported by multiple layers of disease phenotype-related databases is essential for deciphering the human genome. To satisfy the impending need to decipher the human genome, it is essential to develop a ...

  5. Unipro UGENE NGS pipelines and components for variant calling, RNA-seq and ChIP-seq data analyses

    OpenAIRE

    Olga Golosova; Ross Henderson; Yuriy Vaskin; Andrei Gabrielian; German Grekhov; Vijayaraj Nagarajan; Oler, Andrew J.; Mariam Quiñones; Darrell Hurt; Mikhail Fursov; Yentram Huyen

    2014-01-01

    The advent of Next Generation Sequencing (NGS) technologies has opened new possibilities for researchers. However, the more biology becomes a data-intensive field, the more biologists have to learn how to process and analyze NGS data with complex computational tools. Even with the availability of common pipeline specifications, it is often a time-consuming and cumbersome task for a bench scientist to install and configure the pipeline tools. We believe that a unified, desktop and biologist-fr...

  6. Chemistry control at Bruce NGS 'B' from constructed to commercial operation

    International Nuclear Information System (INIS)

    Pre-operational storage chemistry and flushing of the secondary side is described. The approach devised for Bruce NGS 'B' Unit 6 was unique for an Ontario Hydro Nuclear Unit. The significance of the improved Construction installation and Quality Assurance procedures, combined with those of Operations is identified. Secondary side chemistry during both commissioning and later operation is reported. It will be shown that the application of ALARA (As Low As is Reasonably Achievable) concept has resulted in tighter chemical specifications being met

  7. A duck RH panel and its potential for assisting NGS genome assembly

    OpenAIRE

    Rao Man; Morisson Mireille; Faraut Thomas; Bardes Suzanne; Fève Katia; Labarthe Emmanuelle; Fillon Valérie; Huang Yinhua; Li Ning; Vignal Alain

    2012-01-01

    Abstract Background Owing to the low cost of the high throughput Next Generation Sequencing (NGS) technology, more and more species have been and will be sequenced. However, de novo assemblies of large eukaryotic genomes thus produced are composed of a large number of contigs and scaffolds of medium to small size, having no chromosomal assignment. Radiation hybrid (RH) mapping is a powerful tool for building whole genome maps and has been used for several animal species, to help assign sequen...

  8. SummonChimera infers integrated viral genomes with nucleotide precision from NGS data

    OpenAIRE

    Katz, Joshua P.; Pipas, James M.

    2014-01-01

    Background Viral integration into a host genome is defined by two chimeric junctions that join viral and host DNA. Recently, computational tools have been developed that utilize NGS data to detect chimeric junctions. These methods identify individual viral-host junctions but do not associate chimeric pairs as an integration event. Without knowing the chimeric boundaries of an integration, its genetic content cannot be determined. Results Summonchimera is a Perl program that associates chimera...

  9. THE INVESTIGATION OF ENDOPHYTIC MICROORGANISMS AS A SOURCE FOR SILAGE MICROBIOCENOSIS FORMATION USING NGS-SEQUENCING

    OpenAIRE

    E.A. YILDIRIM; G.Yu. LAPTEV; L.A. IL’INA; I.N. NIKONOV; V.A. FILIPPOVA; V.V. SOLDATOVA; E.A. BRAZHNIK; N.I. NOVIKOVA; T.YU. GAGKAEVA

    2015-01-01

    The composition of plant and silage microflora affects the fermentation processes in the silage and its final quality. To date, reports about studying fodder plants and silage microbiota by means of molecular genetic methods are few and limited to descriptions of composition and function of some groups of microorganisms. Moreover, the NGS (next generation sequencing) data on diversity of epiphytic microflora and silage microbiocoenosis are not still reported. We first used this approach in st...

  10. Best effort analysis of critical large loss-of-coolant accident in Darlington NGS

    International Nuclear Information System (INIS)

    A best-effort analysis of Emergency Coolant Injection System (ECIS) effectiveness has been performed for a critical large break loss of coolant accident (LOCA) in Darlington NGS. This analysis, and various sensitivity analyses were performed using the best-effort version of the TUF two-fluid thermal-hydraulics code. The objective of this project is to develop analytical tools and analysis methodology to quantify, within reasonable bounds of certainty, the effectiveness of the ECIS in Ontario Hydro nuclear generating stations to limit activity releases from fuel in the event of a large break LOCA. As part of Best Effort ECIS effectiveness methodology, and the pilot application of this methodology to the analysis of Large LOCA for Darlington NGS, the TUF code has been developed to: quantify the degree of blowdown cooling in a multiple parallel channel reactor core; establish the minimum moderator subcooling required to ensure that fuel channel integrity is maintained, and determine the maximum time that the moderator is required to act as a heat sink; quantify the effectiveness of the ECIS to limit the extent of fuel and fuel channel heatup. The methodology described in this paper, together with enhancements to account for the effects of fuel string relocation, higher void reactivity uncertainty allowance and flux tilt on the initial overpower transient, has been implemented in the Generic Safety Report analysis to update the Large LOCA Safety Report sections for the Bruce and Pickering NGS. (author). 9 refs., 12 figs

  11. HLA-genotyping of clinical specimens using Ion Torrent-based NGS.

    Science.gov (United States)

    Barone, Jonathan C; Saito, Katsuyuki; Beutner, Karl; Campo, Maria; Dong, Wei; Goswami, Chirayu P; Johnson, Erica S; Wang, Zi-Xuan; Hsu, Susan

    2015-12-01

    We have evaluated and validated the NXType™ workflow (One Lambda, Inc.) and the accompanying TypeStream™ software on the Ion Torrent Next Generation Sequencing (NGS) platform using a comprehensive testing panel. The panel consisted of 285 genomic DNA (gDNA) samples derived from four major ethnic populations and contained 59 PT samples and 226 clinical specimens. The total number of alleles from the six loci interrogated by NGS was 3420. This validation panel provided a wide range of HLA sequence variations including many rare alleles, new variants and homozygous alleles. The NXType™ system (reagents and software) was able to correctly genotype the vast majority of these specimens. The concordance rate between SBT-derived genotypes and those generated by TypeStream™ auto-analysis ranged from 99.5% to 99.8% for the HLA-A, B, C, DRB1 and DQB1 loci, and was 98.9% for HLA-DPB1. A strategy for data review was developed that would allow correction of most of the few remaining typing errors. The entire NGS workflow from gDNA amplification to genotype assignment could be completed within 3 working days. Through this validation study, the limitations and shortcomings of the platform, specific assay system, and software algorithm were also revealed for further evaluation and improvement.

  12. The variation game: Cracking complex genetic disorders with NGS and omics data.

    Science.gov (United States)

    Cui, Hongzhu; Dhroso, Andi; Johnson, Nathan; Korkin, Dmitry

    2015-06-01

    Tremendous advances in Next Generation Sequencing (NGS) and high-throughput omics methods have brought us one step closer towards mechanistic understanding of the complex disease at the molecular level. In this review, we discuss four basic regulatory mechanisms implicated in complex genetic diseases, such as cancer, neurological disorders, heart disease, diabetes, and many others. The mechanisms, including genetic variations, copy-number variations, posttranscriptional variations, and epigenetic variations, can be detected using a variety of NGS methods. We propose that malfunctions detected in these mechanisms are not necessarily independent, since these malfunctions are often found associated with the same disease and targeting the same gene, group of genes, or functional pathway. As an example, we discuss possible rewiring effects of the cancer-associated genetic, structural, and posttranscriptional variations on the protein-protein interaction (PPI) network centered around P53 protein. The review highlights multi-layered complexity of common genetic disorders and suggests that integration of NGS and omics data is a critical step in developing new computational methods capable of deciphering this complexity.

  13. Parallel WGA and WTA for Comparative Genome and Transcriptome NGS Analysis Using Tiny Cell Numbers.

    Science.gov (United States)

    Korfhage, Christian; Fricke, Evelyn; Meier, Andreas

    2015-07-01

    Genomic DNA determines how and when the transcriptome is changed by a trigger or environmental change and how cellular metabolism is influenced. Comparative genome and transcriptome analysis of the same cell sample links a defined genome with all changes in the bases, structure, or numbers of the transcriptome. However, comparative genome and transcriptome analysis using next-generation sequencing (NGS) or real-time PCR is often limited by the small amount of sample available. In mammals, the amount of DNA and RNA in a single cell is ∼10 picograms, but deep analysis of the genome and transcriptome currently requires several hundred nanograms of nucleic acids for library preparation for NGS sequencing. Consequently, accurate whole-genome amplification (WGA) and whole-transcriptome amplification (WTA) is required for such quantitative analysis. This unit describes how the genome and the transcriptome of a tiny number of cells can be amplified in a highly parallel and comparable process. Protocols for quality control of amplified DNA and application of amplified DNA for NGS are included.

  14. Functional genomics of tomato: opportunities and challenges in post-genome NGS era.

    Science.gov (United States)

    Kumar, Rahul; Khurana, Ashima

    2014-12-01

    The Tomato Genome Sequencing Project represented a landmark venture in the history of sequencing projects where both Sanger's and next-generation sequencing (NGS) technologies were employed, and a highly accurate and one of the best assembled plant genomes along with a draft of the wild relative, Solanum pimpinellifolium, were released in 2012. However, the functional potential of the major portion of this newly generated resource is still undefined. The very first challenge before scientists working on tomato functional biology is to exploit this high-quality reference sequence for tapping of the wealth of genetic variants for improving agronomic traits in cultivated tomatoes. The sequence data generated recently by 150 Tomato Genome Consortium would further uncover the natural alleles present in different tomato genotypes. Therefore, we found it relevant to have a fresh outlook on tomato functional genomics in the context of application of NGS technologies in its post-genome sequencing phase. Herein, we provide an overview how NGS technologies vis-a-vis available reference sequence have assisted each other for their mutual improvement and how their combined use could further facilitate the development of other 'omics' tools, required to propel the Solanaceae research. Additionally, we highlight the challenges associated with the application of these cutting-edge technologies.

  15. tropiTree: an NGS-based EST-SSR resource for 24 tropical tree species.

    Science.gov (United States)

    Russell, Joanne R; Hedley, Peter E; Cardle, Linda; Dancey, Siobhan; Morris, Jenny; Booth, Allan; Odee, David; Mwaura, Lucy; Omondi, William; Angaine, Peter; Machua, Joseph; Muchugi, Alice; Milne, Iain; Kindt, Roeland; Jamnadass, Ramni; Dawson, Ian K

    2014-01-01

    The development of genetic tools for non-model organisms has been hampered by cost, but advances in next-generation sequencing (NGS) have created new opportunities. In ecological research, this raises the prospect for developing molecular markers to simultaneously study important genetic processes such as gene flow in multiple non-model plant species within complex natural and anthropogenic landscapes. Here, we report the use of bar-coded multiplexed paired-end Illumina NGS for the de novo development of expressed sequence tag-derived simple sequence repeat (EST-SSR) markers at low cost for a range of 24 tree species. Each chosen tree species is important in complex tropical agroforestry systems where little is currently known about many genetic processes. An average of more than 5,000 EST-SSRs was identified for each of the 24 sequenced species, whereas prior to analysis 20 of the species had fewer than 100 nucleotide sequence citations. To make results available to potential users in a suitable format, we have developed an open-access, interactive online database, tropiTree (http://bioinf.hutton.ac.uk/tropiTree), which has a range of visualisation and search facilities, and which is a model for the efficient presentation and application of NGS data.

  16. Functional genomics of tomato: Opportunities and challenges in post-genome NGS era

    Indian Academy of Sciences (India)

    Rahul Kumar; Ashima Khurana

    2014-12-01

    The Tomato Genome Sequencing Project represented a landmark venture in the history of sequencing projects where both Sanger’s and next-generation sequencing (NGS) technologies were employed, and a highly accurate and one of the best assembled plant genomes along with a draft of the wild relative, Solanum pimpinellifolium, were released in 2012. However, the functional potential of the major portion of this newly generated resource is still undefined. The very first challenge before scientists working on tomato functional biology is to exploit this high-quality reference sequence for tapping of the wealth of genetic variants for improving agronomic traits in cultivated tomatoes. The sequence data generated recently by 150 Tomato Genome Consortium would further uncover the natural alleles present in different tomato genotypes. Therefore, we found it relevant to have a fresh outlook on tomato functional genomics in the context of application of NGS technologies in its post-genome sequencing phase. Herein, we provide an overview how NGS technologies vis-à-vis available reference sequence have assisted each other for their mutual improvement and how their combined use could further facilitate the development of other ‘omics’ tools, required to propel the Solanaceae research. Additionally, we highlight the challenges associated with the application of these cutting-edge technologies.

  17. Next-generation sequencing (NGS) for assessment of microbial water quality: current progress, challenges, and future opportunities.

    Science.gov (United States)

    Tan, BoonFei; Ng, Charmaine; Nshimyimana, Jean Pierre; Loh, Lay Leng; Gin, Karina Y-H; Thompson, Janelle R

    2015-01-01

    Water quality is an emergent property of a complex system comprised of interacting microbial populations and introduced microbial and chemical contaminants. Studies leveraging next-generation sequencing (NGS) technologies are providing new insights into the ecology of microbially mediated processes that influence fresh water quality such as algal blooms, contaminant biodegradation, and pathogen dissemination. In addition, sequencing methods targeting small subunit (SSU) rRNA hypervariable regions have allowed identification of signature microbial species that serve as bioindicators for sewage contamination in these environments. Beyond amplicon sequencing, metagenomic and metatranscriptomic analyses of microbial communities in fresh water environments reveal the genetic capabilities and interplay of waterborne microorganisms, shedding light on the mechanisms for production and biodegradation of toxins and other contaminants. This review discusses the challenges and benefits of applying NGS-based methods to water quality research and assessment. We will consider the suitability and biases inherent in the application of NGS as a screening tool for assessment of biological risks and discuss the potential and limitations for direct quantitative interpretation of NGS data. Secondly, we will examine case studies from recent literature where NGS based methods have been applied to topics in water quality assessment, including development of bioindicators for sewage pollution and microbial source tracking, characterizing the distribution of toxin and antibiotic resistance genes in water samples, and investigating mechanisms of biodegradation of harmful pollutants that threaten water quality. Finally, we provide a short review of emerging NGS platforms and their potential applications to the next generation of water quality assessment tools.

  18. An approach to the proposal of an annual limit of intake for the general public (ALIgp) for organically bound tritium

    International Nuclear Information System (INIS)

    Values for ALIs for the general public for adults, newborn, 1 and 10 year old children are proposed. These are based on a simple two component model in which all the ingested tritium is assumed to be absorbed and 50% enters carbon-hydrogen bonds from which it is released with the biological half-time of carbon; the remaining 50% is assumed to equilibrate rapidly with the total body water pool. (author)

  19. 45 CFR 1176.5 - Annual plan.

    Science.gov (United States)

    2010-10-01

    ... ENDOWMENT FOR THE HUMANITIES PART-TIME CAREER EMPLOYMENT § 1176.5 Annual plan. (a) An agencywide plan for promoting part-time employment opportunities will be developed annually. This plan will establish annual... 45 Public Welfare 3 2010-10-01 2010-10-01 false Annual plan. 1176.5 Section 1176.5 Public...

  20. Special challenges for public health with climate change and aging populations: Waterborne illness - 20th Annual John K. Friesen Conference - Growing Old in a Changing Climate: Exploring the Interface Between Population Aging and Global Warming (2011)

    OpenAIRE

    Takaro, Tim

    2011-01-01

    This video clip comprises the four presentations of Panel Session 4, “Preparing Aging Populations for Climate Change in British Columbia and Beyond” held at the 20th Annual John K. Friesen Conference, "Growing Old in a Changing Climate: Exploring the Interface Between Population Aging and Global Warming," MAY 25-26, 2011, Vancouver, BC. Dr. Tim Takaro "Special challenges for public health with climate change and aging populations: Waterborne illness" - Climate change is causing public health ...

  1. Research to support public health action on heat and health - 20th Annual John K. Friesen Conference - Growing Old in a Changing Climate: Exploring the Interface Between Population Aging and Global Warming (2011)

    OpenAIRE

    Kosatsky, Tom

    2011-01-01

    This video clip comprises the four presentations of Panel Session 2, “Mitigation and Prevention Strategies: Lessons Learned on the Front Lines” held at the 20th Annual John K. Friesen Conference, "Growing Old in a Changing Climate: Exploring the Interface Between Population Aging and Global Warming," MAY 25-26, 2011, Vancouver, BC. Dr. Tom Kosatsky " Research to support public health action on heat and health" - Research from various disciplines can promote, support and contextualize public h...

  2. Annual report 1980

    International Nuclear Information System (INIS)

    This annual report contains a description of the named institute, the research programm, reports from the scientific establishments, a description of different cooperations, and a list of scientific publications. (HSI)

  3. The Implications for Higher-Accuracy Absolute Measurements for NGS and its GRAV-D Project

    Science.gov (United States)

    Childers, V. A.; Winester, D.; Roman, D. R.; Eckl, M. C.; Smith, D. A.

    2013-12-01

    Absolute and relative gravity measurements play an important role in the work of NOAA's National Geodetic Survey (NGS). When NGS decided to replace the US national vertical datum, the Gravity for the Redefinition of the American Vertical Datum (GRAV-D) project added a new dimension to the NGS gravity program. Airborne gravity collection would complement existing satellite and surface gravity data to allow the creation of a gravimetric geoid sufficiently accurate to form the basis of the new reference surface. To provide absolute gravity ties for the airborne surveys, initially new FG5 absolute measurements were made at existing absolute stations and relative measurements were used to transfer those measurements to excenters near the absolute mark and to the aircraft sensor height at the parking space. In 2011, NGS obtained a field-capable A10 absolute gravimeter from Micro-g LaCoste which became the basis of the support of the airborne surveys. Now A10 measurements are made at the aircraft location and transferred to sensor height. Absolute and relative gravity play other roles in GRAV-D. Comparison of surface data with new airborne collection will highlight surface surveys with bias or tilt errors and can provide enough information to repair or discard the data. We expect that areas of problem surface data may be re-measured. The GRAV-D project also plans to monitor the geoid in regions of rapid change and update the vertical datum when appropriate. Geoid change can result from glacial isostatic adjustment (GIA), tectonic change, and the massive drawdown of large scale aquifers. The NGS plan for monitoring these changes over time is still in its preliminary stages and is expected to rely primarily on the GRACE and GRACE Follow On satellite data in conjunction with models of GIA and tectonic change. We expect to make absolute measurements in areas of rapid change in order to verify model predictions. With the opportunities presented by rapid, highly accurate

  4. A high-throughput optomechanical retrieval method for sequence-verified clonal DNA from the NGS platform.

    Science.gov (United States)

    Lee, Howon; Kim, Hyoki; Kim, Sungsik; Ryu, Taehoon; Kim, Hwangbeom; Bang, Duhee; Kwon, Sunghoon

    2015-02-02

    Writing DNA plays a significant role in the fields of synthetic biology, functional genomics and bioengineering. DNA clones on next-generation sequencing (NGS) platforms have the potential to be a rich and cost-effective source of sequence-verified DNAs as a precursor for DNA writing. However, it is still very challenging to retrieve target clonal DNA from high-density NGS platforms. Here we propose an enabling technology called 'Sniper Cloning' that enables the precise mapping of target clone features on NGS platforms and non-contact rapid retrieval of targets for the full utilization of DNA clones. By merging the three cutting-edge technologies of NGS, DNA microarray and our pulse laser retrieval system, Sniper Cloning is a week-long process that produces 5,188 error-free synthetic DNAs in a single run of NGS with a single microarray DNA pool. We believe that this technology has potential as a universal tool for DNA writing in biological sciences.

  5. Unipro UGENE NGS pipelines and components for variant calling, RNA-seq and ChIP-seq data analyses.

    Science.gov (United States)

    Golosova, Olga; Henderson, Ross; Vaskin, Yuriy; Gabrielian, Andrei; Grekhov, German; Nagarajan, Vijayaraj; Oler, Andrew J; Quiñones, Mariam; Hurt, Darrell; Fursov, Mikhail; Huyen, Yentram

    2014-01-01

    The advent of Next Generation Sequencing (NGS) technologies has opened new possibilities for researchers. However, the more biology becomes a data-intensive field, the more biologists have to learn how to process and analyze NGS data with complex computational tools. Even with the availability of common pipeline specifications, it is often a time-consuming and cumbersome task for a bench scientist to install and configure the pipeline tools. We believe that a unified, desktop and biologist-friendly front end to NGS data analysis tools will substantially improve productivity in this field. Here we present NGS pipelines "Variant Calling with SAMtools", "Tuxedo Pipeline for RNA-seq Data Analysis" and "Cistrome Pipeline for ChIP-seq Data Analysis" integrated into the Unipro UGENE desktop toolkit. We describe the available UGENE infrastructure that helps researchers run these pipelines on different datasets, store and investigate the results and re-run the pipelines with the same parameters. These pipeline tools are included in the UGENE NGS package. Individual blocks of these pipelines are also available for expert users to create their own advanced workflows.

  6. Sample Results From The Extraction, Scrub, And Strip Test For The Blended NGS Solvent

    International Nuclear Information System (INIS)

    This report summarizes the results of the extraction, scrub, and strip testing for the September 2013 sampling of the Next Generation Solvent (NGS) Blended solvent from the Modular Caustic Side-Solvent Extraction Unit (MCU) Solvent Hold Tank. MCU is in the process of transitioning from the BOBCalixC6 solvent to the NGS Blend solvent. As part of that transition, MCU has intentionally created a blended solvent to be processed using the Salt Batch program. This sample represents the first sample received from that blended solvent. There were two ESS tests performed where NGS blended solvent performance was assessed using either the Tank 21 material utilized in the Salt Batch 7 analyses or a simulant waste material used in the V-5/V-10 contactor testing. This report tabulates the temperature corrected cesium distribution, or DCs values, step recovery percentage, and actual temperatures recorded during the experiment. This report also identifies the sample receipt date, preparation method, and analysis performed in the accumulation of the listed values. The calculated extraction DCs values using the Tank 21H material and simulant are 59.4 and 53.8, respectively. The DCs values for two scrub and three strip processes for the Tank 21 material are 4.58, 2.91, 0.00184, 0.0252, and 0.00575, respectively. The D-values for two scrub and three strip processes for the simulant are 3.47, 2.18, 0.00468, 0.00057, and 0.00572, respectively. These values are similar to previous measurements of Salt Batch 7 feed with lab-prepared blended solvent. These numbers are considered compatible to allow simulant testing to be completed in place of actual waste due to the limited availability of feed material

  7. Sample Results From The Extraction, Scrub, And Strip Test For The Blended NGS Solvent

    Energy Technology Data Exchange (ETDEWEB)

    Washington, A. L. II; Peters, T. B.

    2014-03-03

    This report summarizes the results of the extraction, scrub, and strip testing for the September 2013 sampling of the Next Generation Solvent (NGS) Blended solvent from the Modular Caustic Side-Solvent Extraction Unit (MCU) Solvent Hold Tank. MCU is in the process of transitioning from the BOBCalixC6 solvent to the NGS Blend solvent. As part of that transition, MCU has intentionally created a blended solvent to be processed using the Salt Batch program. This sample represents the first sample received from that blended solvent. There were two ESS tests performed where NGS blended solvent performance was assessed using either the Tank 21 material utilized in the Salt Batch 7 analyses or a simulant waste material used in the V-5/V-10 contactor testing. This report tabulates the temperature corrected cesium distribution, or DCs values, step recovery percentage, and actual temperatures recorded during the experiment. This report also identifies the sample receipt date, preparation method, and analysis performed in the accumulation of the listed values. The calculated extraction DCs values using the Tank 21H material and simulant are 59.4 and 53.8, respectively. The DCs values for two scrub and three strip processes for the Tank 21 material are 4.58, 2.91, 0.00184, 0.0252, and 0.00575, respectively. The D-values for two scrub and three strip processes for the simulant are 3.47, 2.18, 0.00468, 0.00057, and 0.00572, respectively. These values are similar to previous measurements of Salt Batch 7 feed with lab-prepared blended solvent. These numbers are considered compatible to allow simulant testing to be completed in place of actual waste due to the limited availability of feed material.

  8. Critical review of NGS analyses for de novo genotyping multigene families.

    Science.gov (United States)

    Lighten, Jackie; van Oosterhout, Cock; Bentzen, Paul

    2014-08-01

    The genotyping of highly polymorphic multigene families across many individuals used to be a particularly challenging task because of methodological limitations associated with traditional approaches. Next-generation sequencing (NGS) can overcome most of these limitations, and it is increasingly being applied in population genetic studies of multigene families. Here, we critically review NGS bioinformatic approaches that have been used to genotype the major histocompatibility complex (MHC) immune genes, and we discuss how the significant advances made in this field are applicable to population genetic studies of gene families. Increasingly, approaches are introduced that apply thresholds of sequencing depth and sequence similarity to separate alleles from methodological artefacts. We explain why these approaches are particularly sensitive to methodological biases by violating fundamental genotyping assumptions. An alternative strategy that utilizes ultra-deep sequencing (hundreds to thousands of sequences per amplicon) to reconstruct genotypes and applies statistical methods on the sequencing depth to separate alleles from artefacts appears to be more robust. Importantly, the 'degree of change' (DOC) method avoids using arbitrary cut-off thresholds by looking for statistical boundaries between the sequencing depth for alleles and artefacts, and hence, it is entirely repeatable across studies. Although the advances made in generating NGS data are still far ahead of our ability to perform reliable processing, analysis and interpretation, the community is developing statistically rigorous protocols that will allow us to address novel questions in evolution, ecology and genetics of multigene families. Future developments in third-generation single molecule sequencing may potentially help overcome problems that still persist in de novo multigene amplicon genotyping when using current second-generation sequencing approaches.

  9. New genes and pathomechanisms in mitochondrial disorders unraveled by NGS technologies.

    Science.gov (United States)

    Legati, Andrea; Reyes, Aurelio; Nasca, Alessia; Invernizzi, Federica; Lamantea, Eleonora; Tiranti, Valeria; Garavaglia, Barbara; Lamperti, Costanza; Ardissone, Anna; Moroni, Isabella; Robinson, Alan; Ghezzi, Daniele; Zeviani, Massimo

    2016-08-01

    Next Generation Sequencing (NGS) technologies are revolutionizing the diagnostic screening for rare disease entities, including primary mitochondrial disorders, particularly those caused by nuclear gene defects. NGS approaches are able to identify the causative gene defects in small families and even single individuals, unsuitable for investigation by traditional linkage analysis. These technologies are contributing to fill the gap between mitochondrial disease cases defined on the basis of clinical, neuroimaging and biochemical readouts, which still outnumber by approximately 50% the cases for which a molecular-genetic diagnosis is attained. We have been using a combined, two-step strategy, based on targeted genes panel as a first NGS screening, followed by whole exome sequencing (WES) in still unsolved cases, to analyze a large cohort of subjects, that failed to show mutations in mtDNA and in ad hoc sets of specific nuclear genes, sequenced by the Sanger's method. Not only this approach has allowed us to reach molecular diagnosis in a significant fraction (20%) of these difficult cases, but it has also revealed unexpected and conceptually new findings. These include the possibility of marked variable penetrance of recessive mutations, the identification of large-scale DNA rearrangements, which explain spuriously heterozygous cases, and the association of mutations in known genes with unusual, previously unreported clinical phenotypes. Importantly, WES on selected cases has unraveled the presence of pathogenic mutations in genes encoding non-mitochondrial proteins (e.g. the transcription factor E4F1), an observation that further expands the intricate genetics of mitochondrial disease and suggests a new area of investigation in mitochondrial medicine. This article is part of a Special Issue entitled 'EBEC 2016: 19th European Bioenergetics Conference, Riva del Garda, Italy, July 2-6, 2016', edited by Prof. Paolo Bernardi.

  10. New genes and pathomechanisms in mitochondrial disorders unraveled by NGS technologies.

    Science.gov (United States)

    Legati, Andrea; Reyes, Aurelio; Nasca, Alessia; Invernizzi, Federica; Lamantea, Eleonora; Tiranti, Valeria; Garavaglia, Barbara; Lamperti, Costanza; Ardissone, Anna; Moroni, Isabella; Robinson, Alan; Ghezzi, Daniele; Zeviani, Massimo

    2016-08-01

    Next Generation Sequencing (NGS) technologies are revolutionizing the diagnostic screening for rare disease entities, including primary mitochondrial disorders, particularly those caused by nuclear gene defects. NGS approaches are able to identify the causative gene defects in small families and even single individuals, unsuitable for investigation by traditional linkage analysis. These technologies are contributing to fill the gap between mitochondrial disease cases defined on the basis of clinical, neuroimaging and biochemical readouts, which still outnumber by approximately 50% the cases for which a molecular-genetic diagnosis is attained. We have been using a combined, two-step strategy, based on targeted genes panel as a first NGS screening, followed by whole exome sequencing (WES) in still unsolved cases, to analyze a large cohort of subjects, that failed to show mutations in mtDNA and in ad hoc sets of specific nuclear genes, sequenced by the Sanger's method. Not only this approach has allowed us to reach molecular diagnosis in a significant fraction (20%) of these difficult cases, but it has also revealed unexpected and conceptually new findings. These include the possibility of marked variable penetrance of recessive mutations, the identification of large-scale DNA rearrangements, which explain spuriously heterozygous cases, and the association of mutations in known genes with unusual, previously unreported clinical phenotypes. Importantly, WES on selected cases has unraveled the presence of pathogenic mutations in genes encoding non-mitochondrial proteins (e.g. the transcription factor E4F1), an observation that further expands the intricate genetics of mitochondrial disease and suggests a new area of investigation in mitochondrial medicine. This article is part of a Special Issue entitled 'EBEC 2016: 19th European Bioenergetics Conference, Riva del Garda, Italy, July 2-6, 2016', edited by Prof. Paolo Bernardi. PMID:26968897

  11. Chemistry control at Bruce N.G.S. 'B' from construction to commercial operation

    International Nuclear Information System (INIS)

    Pre-operational storage chemistry and flushing of the secondary side is described. The approach devised for Bruce N.G.S. 'B' Unit 6 was unique for an Ontario Hydro nuclear unit. The significance of the improved construction installation and quality assurance is identified. Secondary side chemistry during both commissioning and later operation is reported. It will be shown that the application of ALARA (As Low As is Reasonably Achievable) concept has resulted in tighter chemical specifications being met. (author). 11 refs., 6 tabs., 4 figs

  12. Fast extraction from SPS LSS4 for the LHC and NGS projects

    CERN Document Server

    Goddard, B

    1998-01-01

    The proton and lead ion beams for the anti-clockwise ring of the LHC will be extracted from the SPS in LSS4 and transferred to LHC point 8 via the transfer line TT40-TI8. A conventional single turn 'fast' extraction will be used. The same extraction channel may be used to transfer protons to a neutrino target for the proposed long baseline Neutrino to Gran Sasso (NGS) project. The planned layout for the extraction channel in LSS4 now has the extraction septum in SPS half period 418, and is outlined for both fast-pulsed and DC septum designs.

  13. Precise GPS ephemerides from DMA and NGS tested by time transfer

    Science.gov (United States)

    Lewandowski, Wlodzimierz W.; Petit, Gerard; Thomas, Claudine

    1992-01-01

    It was shown that the use of the Defense Mapping Agency's (DMA) precise ephemerides brings a significant improvement to the accuracy of GPS time transfer. At present a new set of precise ephemerides produced by the National Geodetic Survey (NGS) has been made available to the timing community. This study demonstrates that both types of precise ephemerides improve long-distance GPS time transfer and remove the effects of Selective Availability (SA) degradation of broadcast ephemerides. The issue of overcoming SA is also discussed in terms of the routine availability of precise ephemerides.

  14. Linking Authenticating and Authorising Infrastructures in the UK NGI (SARoNGS)

    OpenAIRE

    Mike A.S. JONES, Jens JENSEN

    2012-01-01

    The UK NGS aims to provide simple trusted access to digital services for the UK’s research community, in particular but not limited to Grid and Cloud provision. To achieve this we have to satisfy conditions laid down by three types of entities: individuals, resources, and the identification and attribute authorities who vouch for them. We have to set the bar high enough to satisfy resource owners, low enough to let most legitimate users in and yet also satisfy legal requirements. This makes it...

  15. A case study for cloud based high throughput analysis of NGS data using the globus genomics system

    Directory of Open Access Journals (Sweden)

    Krithika Bhuvaneshwar

    2015-01-01

    Full Text Available Next generation sequencing (NGS technologies produce massive amounts of data requiring a powerful computational infrastructure, high quality bioinformatics software, and skilled personnel to operate the tools. We present a case study of a practical solution to this data management and analysis challenge that simplifies terabyte scale data handling and provides advanced tools for NGS data analysis. These capabilities are implemented using the “Globus Genomics” system, which is an enhanced Galaxy workflow system made available as a service that offers users the capability to process and transfer data easily, reliably and quickly to address end-to-endNGS analysis requirements. The Globus Genomics system is built on Amazon's cloud computing infrastructure. The system takes advantage of elastic scaling of compute resources to run multiple workflows in parallel and it also helps meet the scale-out analysis needs of modern translational genomics research.

  16. Next generation sequencing (NGS) database for tandem repeats with multiple pattern 2°-shaft multicore string matching.

    Science.gov (United States)

    Someswara Rao, Chinta; Raju, S Viswanadha

    2016-03-01

    Next generation sequencing (NGS) technologies have been rapidly applied in biomedical and biological research in recent years. To provide the comprehensive NGS resource for the research, in this paper , we have considered 10 loci/codi/repeats TAGA, TCAT, GAAT, AGAT, AGAA, GATA, TATC, CTTT, TCTG and TCTA. Then we developed the NGS Tandem Repeat Database (TandemRepeatDB) for all the chromosomes of Homo sapiens, Callithrix jacchus, Chlorocebus sabaeus, Gorilla gorilla, Macaca fascicularis, Macaca mulatta, Nomascus leucogenys, Pan troglodytes, Papio anubis and Pongo abelii genome data sets for all those locis. We find the successive occurence frequency for all the above 10 SSR (simple sequence repeats) in the above genome data sets on a chromosome-by-chromosome basis with multiple pattern 2° shaft multicore string matching.

  17. Next generation sequencing (NGS) database for tandem repeats with multiple pattern 2°-shaft multicore string matching

    Science.gov (United States)

    Someswara Rao, Chinta; Raju, S. Viswanadha

    2016-01-01

    Next generation sequencing (NGS) technologies have been rapidly applied in biomedical and biological research in recent years. To provide the comprehensive NGS resource for the research, in this paper , we have considered 10 loci/codi/repeats TAGA, TCAT, GAAT, AGAT, AGAA, GATA, TATC, CTTT, TCTG and TCTA. Then we developed the NGS Tandem Repeat Database (TandemRepeatDB) for all the chromosomes of Homo sapiens, Callithrix jacchus, Chlorocebus sabaeus, Gorilla gorilla, Macaca fascicularis, Macaca mulatta, Nomascus leucogenys, Pan troglodytes, Papio anubis and Pongo abelii genome data sets for all those locis. We find the successive occurence frequency for all the above 10 SSR (simple sequence repeats) in the above genome data sets on a chromosome-by-chromosome basis with multiple pattern 2° shaft multicore string matching. PMID:26981434

  18. Next generation sequencing (NGS) database for tandem repeats with multiple pattern 2°-shaft multicore string matching.

    Science.gov (United States)

    Someswara Rao, Chinta; Raju, S Viswanadha

    2016-03-01

    Next generation sequencing (NGS) technologies have been rapidly applied in biomedical and biological research in recent years. To provide the comprehensive NGS resource for the research, in this paper , we have considered 10 loci/codi/repeats TAGA, TCAT, GAAT, AGAT, AGAA, GATA, TATC, CTTT, TCTG and TCTA. Then we developed the NGS Tandem Repeat Database (TandemRepeatDB) for all the chromosomes of Homo sapiens, Callithrix jacchus, Chlorocebus sabaeus, Gorilla gorilla, Macaca fascicularis, Macaca mulatta, Nomascus leucogenys, Pan troglodytes, Papio anubis and Pongo abelii genome data sets for all those locis. We find the successive occurence frequency for all the above 10 SSR (simple sequence repeats) in the above genome data sets on a chromosome-by-chromosome basis with multiple pattern 2° shaft multicore string matching. PMID:26981434

  19. Research laboratories annual report 1994

    International Nuclear Information System (INIS)

    The publication is the 1994 annual report of the Israel atomic energy commission in a new format. The report includes three invited papers and a bibliographic list of publications by the commission scientific researches

  20. Next-generation sequencing (NGS for assessment of microbial water quality: current progress, challenges, and future opportunities

    Directory of Open Access Journals (Sweden)

    BoonFei eTan

    2015-09-01

    Full Text Available Water quality is an emergent property of a complex system comprised of interacting microbial populations and introduced microbial and chemical contaminants. Studies leveraging next-generation sequencing (NGS technologies are providing new insights into the ecology of microbially mediated processes that influence fresh water quality such as algal blooms, contaminant biodegradation, and pathogen dissemination. In addition, sequencing methods targeting small subunit (SSU rRNA hypervariable regions have allowed identification of signature microbial species that serve as bioindicators for sewage contamination in these environments. Beyond amplicon sequencing, metagenomic and metatranscriptomic analyses of microbial communities in fresh water environments reveal the genetic capabilities and interplay of waterborne microorganisms, shedding light on the mechanisms for production and biodegradation of toxins and other contaminants. This review discusses the challenges and benefits of applying NGS-based methods to water quality research and assessment. We will consider the suitability and biases inherent in the application of NGS as a screening tool for assessment of biological risks and discuss the potential and limitations for direct quantitative interpretation of NGS data. Secondly, we will examine case studies from recent literature where NGS based methods have been applied to topics in water quality assessment, including development of bioindicators for sewage pollution and microbial source tracking, characterizing the distribution of toxin and antibiotic resistance genes in water samples, and investigating mechanisms of biodegradation of harmful pollutants that threaten water quality. Finally, we provide a short review of emerging NGS platforms and their potential applications to the next generation of water quality assessment tools.

  1. Characterization of oxides on Bruce A NGS liner tubes and steam generator tubes

    International Nuclear Information System (INIS)

    Oxide deposits on end-fitting liner tubes and steam generator tubes from the Bruce A Nuclear Generating Station (NGS) were characterized in advance of the decontamination of the heat transport system (HTS) of Bruce Unit 2. Oxide loadings, and Co-60 surface activities and specific activities were determined for the oxides on inlet and outlet end-fitting liner tubes from Bruce Unit l, Bruce Unit 2 and Bruce Unit 4. Oxides on the inner surfaces of steam generator tubes from Bruce NGS Units 1 and 2 were also characterized. The consistency in the deposit characteristics on the inlet liner tubes and steam generator tubes from Bruce A, along with the absence of magnetite on the outlet liner tubes has led to the development of a model for iron transport in the HTS of pressurized heavy water reactors (PHWRs). The activity transport/fouling mechanism involves flow-accelerated corrosion of the outlet feeder pipes, followed by deposition of iron in the steam generators, along the inlet feeder pipes, on the inlet end fittings, on the inlet fuel bundles and on the inlet region of the pressure tube. The results of loop experiments using decontamination solutions indicated that the oxide was rapidly removed from inlet liner tubes. However, removal of the Cr-rich oxide from the outlet liner tubes was less efficient, requiring the Alkaline Permangante (AP) oxidizing pre-treatment that is typically used in light water reactors (LWRs). The steam generator tubes were effectively decontaminated

  2. The impact of environmental qualification of safety related equipment on future maintenance activities at Darlington NGS

    International Nuclear Information System (INIS)

    CANDU nuclear station owners are faced with and increasing amount of equipment considered to be safety related and required to operate successfully during common mode design basis events (CMDBE), such as earthquakes, loss of coolant accidents, main steam line breaks, etc. Designers are faced with the task of either protecting this equipment from the consequence of the events, or supplying equipment which will operate in the environment expected during these events. This trend has continued at Darlington Nuclear Generating Station (NGS) with the identification of 125 safety related systems which must perform a function during or after the CMDBE. In addition to protecting or hardening the safety related equipment, the designers must also provide assurance that the equipment is capable of operating reliably during any of these events. At Darlington NGS, the effects of aging mechanisms on components of safety related equipment is also being taken into account. This paper describes the Darlington Environmental Qualification Program, and discusses how it will change the nature of safety related equipment maintenance, surveillance, and replacement programs

  3. RV39-another SEBIM group pilot operated relief valve for Darlington NGS

    International Nuclear Information System (INIS)

    Events at some CANDU plants (Pickering, Wolsung, etc) as well as theoretical studies outlined the hazards of spring loaded relief valve instability (chattering or floating) when required to respond to overpressure producing transients other than the one which provided the design conditions for the valve. The problem was initially associated with incidence during which the liquid relief valves (LRV) failed to open. The affected relief valves, in such occasions, were the Bleed Condenser shell side RV's. Darlington NGS decided in 1996 to resolve the problem by using pilot operated tandem valves, manufactured by SEBIM Group from France. An extensive laboratory testing program was implemented followed by analysis based om computer simulation of transients and valve response. The tandem would satisfy all requirements. Another valve, 33310-RV39, on the tube side of the Bleed Condenser was identified in 1999 for replacement for similar reasons i.e. potential to chatter. In 2001 a test program for the valve was designed and implemented and calculations prepared in order to support the application. The advantages of using the SEBIM pilot operated valve for overpressure protection of the Bleed Condenser reflux circuit in Darlington NGS are as follows: 1. Very good performance under various process conditions and independence of transient scenarios; 2. Adequate protection under all foreseeable conditions; 3. Excellent reliability as proved by experience in Darlington and other facilities (author)

  4. Layup chemistry during Pickering NGS units 1 and 2 fuel channel replacement

    International Nuclear Information System (INIS)

    The failure of a heat transport (HT) system pressure tube (PT) in Pickering NGS Unit 2 resulted in a decision to replace all PTs in Pickering NGS Units 1 and 2. Consequently, it became mandatory to protect the various process systems of each unit from deterioration for several years. The activity of storing and monitoring these systems was termed layup. Of all systems, the most important and troublesome components to layup were the steam generators. This was due to the unexpected decomposition of hydrazine to ammonia in demineralized water at ambient temperature. For successful layup it is concluded that: frequent monitoring, typically weekly, of layup chemistry is required to assure control; maintenance activities need to be carefully coordinated to maintain a good layup state; the environmental aspects and reactions of chemicals used for corrosion protection must be carefully considered prior to use in a layup program; compatibility of chemicals with system materials must be carefully considered prior to use in a layup program; and procedures, equipment and facilities necessary to establish a layup state should be available before the need arises to put a generating unit in layup. (author). 10 refs., 2 tabs., 3 figs

  5. NGS-Based Assay for the Identification of Individuals Carrying Recessive Genetic Mutations in Reproductive Medicine.

    Science.gov (United States)

    Abulí, Anna; Boada, Montserrat; Rodríguez-Santiago, Benjamín; Coroleu, Buenaventura; Veiga, Anna; Armengol, Lluís; Barri, Pedro N; Pérez-Jurado, Luis A; Estivill, Xavier

    2016-06-01

    Next-generation sequencing (NGS) has the capacity of carrier screening in gamete donation (GD) programs. We have developed and validated an NGS carrier-screening test (qCarrier test) that includes 200 genes associated with 368 disorders (277 autosomal recessive and 37 X-linked). Carrier screening is performed on oocyte donation candidates and the male partner of oocyte recipient. Carriers of X-linked conditions are excluded from the GD program, whereas donors are chosen who do not carry mutations for the same gene/disease as the recipients. The validation phase showed a high sensitivity (>99% sensitivity) detecting all single-nucleotide variants, 13 indels, and 25 copy-number variants included in the validation set. A total of 1,301 individuals were analysed with the qCarrier test, including 483 candidate oocyte donors and 635 receptor couples, 105 females receiving sperm donation, and 39 couples seeking pregnancy. We identified 56% of individuals who are carriers for at least one genetic condition and 1.7% of female donors who were excluded from the program due to a carrier state of X-linked conditions. Globally, 3% of a priori assigned donations had a high reproductive risk that could be minimized after testing. Genetic counselling at different stages is essential for helping to facilitate a successful and healthy pregnancy.

  6. Integrated Systems for NGS Data Management and Analysis: Open Issues and Available Solutions.

    Science.gov (United States)

    Bianchi, Valerio; Ceol, Arnaud; Ogier, Alessandro G E; de Pretis, Stefano; Galeota, Eugenia; Kishore, Kamal; Bora, Pranami; Croci, Ottavio; Campaner, Stefano; Amati, Bruno; Morelli, Marco J; Pelizzola, Mattia

    2016-01-01

    Next-generation sequencing (NGS) technologies have deeply changed our understanding of cellular processes by delivering an astonishing amount of data at affordable prices; nowadays, many biology laboratories have already accumulated a large number of sequenced samples. However, managing and analyzing these data poses new challenges, which may easily be underestimated by research groups devoid of IT and quantitative skills. In this perspective, we identify five issues that should be carefully addressed by research groups approaching NGS technologies. In particular, the five key issues to be considered concern: (1) adopting a laboratory management system (LIMS) and safeguard the resulting raw data structure in downstream analyses; (2) monitoring the flow of the data and standardizing input and output directories and file names, even when multiple analysis protocols are used on the same data; (3) ensuring complete traceability of the analysis performed; (4) enabling non-experienced users to run analyses through a graphical user interface (GUI) acting as a front-end for the pipelines; (5) relying on standard metadata to annotate the datasets, and when possible using controlled vocabularies, ideally derived from biomedical ontologies. Finally, we discuss the currently available tools in the light of these issues, and we introduce HTS-flow, a new workflow management system conceived to address the concerns we raised. HTS-flow is able to retrieve information from a LIMS database, manages data analyses through a simple GUI, outputs data in standard locations and allows the complete traceability of datasets, accompanying metadata and analysis scripts.

  7. AECB staff review of Bruce NGS 'A' operation for the year 1989

    International Nuclear Information System (INIS)

    The operation of the Bruce Nuclear Generating Station 'B' is monitored and licensing requirements are enforced by the Atomic Energy Control Board (AECB). This report records the conclusions of the AECB staff assessment of Bruce NGS 'A' during 1989 and the early part of 1990. Overall operation of the station met acceptable safety standards. Despite numerous problems and technical difficulties encountered, station management and supervisory personnel acted with due caution and made decisions in the interests of safety. There was evidence of improvement in a number of key areas, supported by pertinent indicators in the objective measures table. The extensive inspection and maintenance programs carried out during the year revealed the extent of component deterioration due to aging to be larger than expected. Hydrogen embrittlement of pressure tubes, erosion/corrosion of steam and feed water valves, heat exchanger tubes and piping, fouling of boilers and heat exchangers, and environmental damage of electrical equipment are examples. Continued aging of plant equipment and its potential for reducing the margins for safe operation must be taken into account by Ontario Hydro in establishing priorities and target dates for completion of actions to resolve identified problems at Bruce NGS 'A'. (2 tabs.)

  8. Integrated Systems for NGS Data Management and Analysis: Open Issues and Available Solutions

    Science.gov (United States)

    Bianchi, Valerio; Ceol, Arnaud; Ogier, Alessandro G. E.; de Pretis, Stefano; Galeota, Eugenia; Kishore, Kamal; Bora, Pranami; Croci, Ottavio; Campaner, Stefano; Amati, Bruno; Morelli, Marco J.; Pelizzola, Mattia

    2016-01-01

    Next-generation sequencing (NGS) technologies have deeply changed our understanding of cellular processes by delivering an astonishing amount of data at affordable prices; nowadays, many biology laboratories have already accumulated a large number of sequenced samples. However, managing and analyzing these data poses new challenges, which may easily be underestimated by research groups devoid of IT and quantitative skills. In this perspective, we identify five issues that should be carefully addressed by research groups approaching NGS technologies. In particular, the five key issues to be considered concern: (1) adopting a laboratory management system (LIMS) and safeguard the resulting raw data structure in downstream analyses; (2) monitoring the flow of the data and standardizing input and output directories and file names, even when multiple analysis protocols are used on the same data; (3) ensuring complete traceability of the analysis performed; (4) enabling non-experienced users to run analyses through a graphical user interface (GUI) acting as a front-end for the pipelines; (5) relying on standard metadata to annotate the datasets, and when possible using controlled vocabularies, ideally derived from biomedical ontologies. Finally, we discuss the currently available tools in the light of these issues, and we introduce HTS-flow, a new workflow management system conceived to address the concerns we raised. HTS-flow is able to retrieve information from a LIMS database, manages data analyses through a simple GUI, outputs data in standard locations and allows the complete traceability of datasets, accompanying metadata and analysis scripts. PMID:27200084

  9. CDH1 mutations in gastric cancer patients from northern Brazil identified by Next- Generation Sequencing (NGS).

    Science.gov (United States)

    El-Husny, Antonette; Raiol-Moraes, Milene; Amador, Marcos; Ribeiro-Dos-Santos, André M; Montagnini, André; Barbosa, Silvanira; Silva, Artur; Assumpção, Paulo; Ishak, Geraldo; Santos, Sidney; Pinto, Pablo; Cruz, Aline; Ribeiro-Dos-Santos, Ândrea

    2016-05-13

    Gastric cancer is considered to be the fifth highest incident tumor worldwide and the third leading cause of cancer deaths. Developing regions report a higher number of sporadic cases, but there are only a few local studies related to hereditary cases of gastric cancer in Brazil to confirm this fact. CDH1 germline mutations have been described both in familial and sporadic cases, but there is only one recent molecular description of individuals from Brazil. In this study we performed Next Generation Sequencing (NGS) to assess CDH1 germline mutations in individuals who match the clinical criteria for Hereditary Diffuse Gastric Cancer (HDGC), or who exhibit very early diagnosis of gastric cancer. Among five probands we detected CDH1 germline mutations in two cases (40%). The mutation c.1023T > G was found in a HDGC family and the mutation c.1849G > A, which is nearly exclusive to African populations, was found in an early-onset case of gastric adenocarcinoma. The mutations described highlight the existence of gastric cancer cases caused by CDH1 germline mutations in northern Brazil, although such information is frequently ignored due to the existence of a large number of environmental factors locally. Our report represent the first CDH1 mutations in HDGC described from Brazil by an NGS platform. PMID:27192129

  10. Integrated systems for NGS data management and analysis: open issues and available solutions

    Directory of Open Access Journals (Sweden)

    Valerio eBianchi

    2016-05-01

    Full Text Available Next-generation sequencing (NGS technologies have deeply changed our understanding of cellular processes by delivering an astonishing amount of data at affordable prices; nowadays, many biology laboratories have already accumulated a large number of sequenced samples. However, managing and analyzing these data poses new challenges, which may easily be underestimated by research groups devoid of IT and quantitative skills. In this perspective, we identify five issues that should be carefully addressed by research groups approaching NGS technologies. In particular, the five key issues to be considered concern: 1 adopting a laboratory management system (LIMS and safeguard the resulting raw data structure in downstream analyses; 2 monitoring the flow of the data and standardizing input and output directories and file names, even when multiple analysis protocols are used on the same data; 3 ensuring complete traceability of the analysis performed; 4 enabling non-experienced users to run analyses through a graphical user interface (GUI acting as a front-end for the pipelines; 5 relying on standard metadata to annotate the datasets, and when possible using controlled vocabularies, ideally derived from biomedical ontologies. Finally, we discuss the currently available tools in the light of these issues, and we introduce HTS-flow, a new workflow management system (WMS conceived to address the concerns we raised. HTS-flow is able to retrieve information from a LIMS database, manages data analyses through a simple GUI, outputs data in standard locations and allows the complete traceability of datasets, accompanying metadata and analysis scripts.

  11. Assessment of the latest NGS enrichment capture methods in clinical context.

    Science.gov (United States)

    García-García, Gema; Baux, David; Faugère, Valérie; Moclyn, Mélody; Koenig, Michel; Claustres, Mireille; Roux, Anne-Françoise

    2016-01-01

    Enrichment capture methods for NGS are widely used, however, they evolve rapidly and it is necessary to periodically measure their strengths and weaknesses before transfer to diagnostic services. We assessed two recently released custom DNA solution-capture enrichment methods for NGS, namely Illumina NRCCE and Agilent SureSelect(QXT), against a reference method NimbleGen SeqCap EZ Choice on a similar gene panel, sharing 678 kb and 110 genes. Two Illumina MiSeq runs of 12 samples each have been performed, for each of the three methods, using the same 24 patients (affected with sensorineural disorders). Technical outcomes have been computed and compared, including depth and evenness of coverage, enrichment in targeted regions, performance in GC-rich regions and ability to generate consistent variant datasets. While we show that the three methods resulted in suitable datasets for standard DNA variant discovery, we describe significant differences between the results for the above parameters. NimbleGen offered the best depth of coverage and evenness, while NRCCE showed the highest on target levels but high duplicate rates. SureSelect(QXT) showed an overall quality close to that of NimbleGen. The new methods exhibit reduced preparation time but behave differently. These findings will guide laboratories in their choice of library enrichment approach. PMID:26864517

  12. Integrated Systems for NGS Data Management and Analysis: Open Issues and Available Solutions.

    Science.gov (United States)

    Bianchi, Valerio; Ceol, Arnaud; Ogier, Alessandro G E; de Pretis, Stefano; Galeota, Eugenia; Kishore, Kamal; Bora, Pranami; Croci, Ottavio; Campaner, Stefano; Amati, Bruno; Morelli, Marco J; Pelizzola, Mattia

    2016-01-01

    Next-generation sequencing (NGS) technologies have deeply changed our understanding of cellular processes by delivering an astonishing amount of data at affordable prices; nowadays, many biology laboratories have already accumulated a large number of sequenced samples. However, managing and analyzing these data poses new challenges, which may easily be underestimated by research groups devoid of IT and quantitative skills. In this perspective, we identify five issues that should be carefully addressed by research groups approaching NGS technologies. In particular, the five key issues to be considered concern: (1) adopting a laboratory management system (LIMS) and safeguard the resulting raw data structure in downstream analyses; (2) monitoring the flow of the data and standardizing input and output directories and file names, even when multiple analysis protocols are used on the same data; (3) ensuring complete traceability of the analysis performed; (4) enabling non-experienced users to run analyses through a graphical user interface (GUI) acting as a front-end for the pipelines; (5) relying on standard metadata to annotate the datasets, and when possible using controlled vocabularies, ideally derived from biomedical ontologies. Finally, we discuss the currently available tools in the light of these issues, and we introduce HTS-flow, a new workflow management system conceived to address the concerns we raised. HTS-flow is able to retrieve information from a LIMS database, manages data analyses through a simple GUI, outputs data in standard locations and allows the complete traceability of datasets, accompanying metadata and analysis scripts. PMID:27200084

  13. CDH1 mutations in gastric cancer patients from northern Brazil identified by Next- Generation Sequencing (NGS).

    Science.gov (United States)

    El-Husny, Antonette; Raiol-Moraes, Milene; Amador, Marcos; Ribeiro-Dos-Santos, André M; Montagnini, André; Barbosa, Silvanira; Silva, Artur; Assumpção, Paulo; Ishak, Geraldo; Santos, Sidney; Pinto, Pablo; Cruz, Aline; Ribeiro-Dos-Santos, Ândrea

    2016-05-13

    Gastric cancer is considered to be the fifth highest incident tumor worldwide and the third leading cause of cancer deaths. Developing regions report a higher number of sporadic cases, but there are only a few local studies related to hereditary cases of gastric cancer in Brazil to confirm this fact. CDH1 germline mutations have been described both in familial and sporadic cases, but there is only one recent molecular description of individuals from Brazil. In this study we performed Next Generation Sequencing (NGS) to assess CDH1 germline mutations in individuals who match the clinical criteria for Hereditary Diffuse Gastric Cancer (HDGC), or who exhibit very early diagnosis of gastric cancer. Among five probands we detected CDH1 germline mutations in two cases (40%). The mutation c.1023T > G was found in a HDGC family and the mutation c.1849G > A, which is nearly exclusive to African populations, was found in an early-onset case of gastric adenocarcinoma. The mutations described highlight the existence of gastric cancer cases caused by CDH1 germline mutations in northern Brazil, although such information is frequently ignored due to the existence of a large number of environmental factors locally. Our report represent the first CDH1 mutations in HDGC described from Brazil by an NGS platform.

  14. NGS-based Molecular diagnosis of 105 eyeGENE(®) probands with Retinitis Pigmentosa.

    Science.gov (United States)

    Ge, Zhongqi; Bowles, Kristen; Goetz, Kerry; Scholl, Hendrik P N; Wang, Feng; Wang, Xinjing; Xu, Shan; Wang, Keqing; Wang, Hui; Chen, Rui

    2015-12-15

    The National Ophthalmic Disease Genotyping and Phenotyping Network (eyeGENE(®)) was established in an effort to facilitate basic and clinical research of human inherited eye disease. In order to provide high quality genetic testing to eyeGENE(®)'s enrolled patients which potentially aids clinical diagnosis and disease treatment, we carried out a pilot study and performed Next-generation sequencing (NGS) based molecular diagnosis for 105 Retinitis Pigmentosa (RP) patients randomly selected from the network. A custom capture panel was designed, which incorporated 195 known retinal disease genes, including 61 known RP genes. As a result, disease-causing mutations were identified in 52 out of 105 probands (solving rate of 49.5%). A total of 82 mutations were identified, and 48 of them were novel. Interestingly, for three probands the molecular diagnosis was inconsistent with the initial clinical diagnosis, while for five probands the molecular information suggested a different inheritance model other than that assigned by the physician. In conclusion, our study demonstrated that NGS target sequencing is efficient and sufficiently precise for molecular diagnosis of a highly heterogeneous patient cohort from eyeGENE(®).

  15. Assessment of the latest NGS enrichment capture methods in clinical context.

    Science.gov (United States)

    García-García, Gema; Baux, David; Faugère, Valérie; Moclyn, Mélody; Koenig, Michel; Claustres, Mireille; Roux, Anne-Françoise

    2016-02-11

    Enrichment capture methods for NGS are widely used, however, they evolve rapidly and it is necessary to periodically measure their strengths and weaknesses before transfer to diagnostic services. We assessed two recently released custom DNA solution-capture enrichment methods for NGS, namely Illumina NRCCE and Agilent SureSelect(QXT), against a reference method NimbleGen SeqCap EZ Choice on a similar gene panel, sharing 678 kb and 110 genes. Two Illumina MiSeq runs of 12 samples each have been performed, for each of the three methods, using the same 24 patients (affected with sensorineural disorders). Technical outcomes have been computed and compared, including depth and evenness of coverage, enrichment in targeted regions, performance in GC-rich regions and ability to generate consistent variant datasets. While we show that the three methods resulted in suitable datasets for standard DNA variant discovery, we describe significant differences between the results for the above parameters. NimbleGen offered the best depth of coverage and evenness, while NRCCE showed the highest on target levels but high duplicate rates. SureSelect(QXT) showed an overall quality close to that of NimbleGen. The new methods exhibit reduced preparation time but behave differently. These findings will guide laboratories in their choice of library enrichment approach.

  16. Panel-based NGS Reveals Novel Pathogenic Mutations in Autosomal Recessive Retinitis Pigmentosa.

    Science.gov (United States)

    Perez-Carro, Raquel; Corton, Marta; Sánchez-Navarro, Iker; Zurita, Olga; Sanchez-Bolivar, Noelia; Sánchez-Alcudia, Rocío; Lelieveld, Stefan H; Aller, Elena; Lopez-Martinez, Miguel Angel; López-Molina, Ma Isabel; Fernandez-San Jose, Patricia; Blanco-Kelly, Fiona; Riveiro-Alvarez, Rosa; Gilissen, Christian; Millan, Jose M; Avila-Fernandez, Almudena; Ayuso, Carmen

    2016-01-25

    Retinitis pigmentosa (RP) is a group of inherited progressive retinal dystrophies (RD) characterized by photoreceptor degeneration. RP is highly heterogeneous both clinically and genetically, which complicates the identification of causative genes and mutations. Targeted next-generation sequencing (NGS) has been demonstrated to be an effective strategy for the detection of mutations in RP. In our study, an in-house gene panel comprising 75 known RP genes was used to analyze a cohort of 47 unrelated Spanish families pre-classified as autosomal recessive or isolated RP. Disease-causing mutations were found in 27 out of 47 cases achieving a mutation detection rate of 57.4%. In total, 33 pathogenic mutations were identified, 20 of which were novel mutations (60.6%). Furthermore, not only single nucleotide variations but also copy-number variations, including three large deletions in the USH2A and EYS genes, were identified. Finally seven out of 27 families, displaying mutations in the ABCA4, RP1, RP2 and USH2A genes, could be genetically or clinically reclassified. These results demonstrate the potential of our panel-based NGS strategy in RP diagnosis.

  17. A high-throughput optomechanical retrieval method for sequence-verified clonal DNA from the NGS platform

    OpenAIRE

    Lee, Howon; Kim, Hyoki; Kim, Sungsik; Ryu, Taehoon; Kim, Hwangbeom; Bang, Duhee; Kwon, Sunghoon

    2015-01-01

    Writing DNA plays a significant role in the fields of synthetic biology, functional genomics and bioengineering. DNA clones on next-generation sequencing (NGS) platforms have the potential to be a rich and cost-effective source of sequence-verified DNAs as a precursor for DNA writing. However, it is still very challenging to retrieve target clonal DNA from high-density NGS platforms. Here we propose an enabling technology called ‘Sniper Cloning’ that enables the precise mapping of target clon...

  18. IgH-V(D)J NGS-MRD measurement pre- and early post-allotransplant defines very low- and very high-risk ALL patients.

    Science.gov (United States)

    Pulsipher, Michael A; Carlson, Chris; Langholz, Bryan; Wall, Donna A; Schultz, Kirk R; Bunin, Nancy; Kirsch, Ilan; Gastier-Foster, Julie M; Borowitz, Michael; Desmarais, Cindy; Williamson, David; Kalos, Michael; Grupp, Stephan A

    2015-05-28

    Positive detection of minimal residual disease (MRD) by multichannel flow cytometry (MFC) prior to hematopoietic cell transplantation (HCT) of patients with acute lymphoblastic leukemia (ALL) identifies patients at high risk for relapse, but many pre-HCT MFC-MRD negative patients also relapse, and the predictive power MFC-MRD early post-HCT is poor. To test whether the increased sensitivity of next-generation sequencing (NGS)-MRD better identifies pre- and post-HCT relapse risk, we performed immunoglobulin heavy chain (IgH) variable, diversity, and joining (V[D]J) DNA sequences J NGS-MRD on 56 patients with B-cell ALL enrolled in Children's Oncology Group trial ASCT0431. NGS-MRD predicted relapse and survival more accurately than MFC-MRD (P NGS-MRD detection was better at predicting relapse than MFC-MRD (P NGS-MRD positive relapse rate, 67%; P = .004). Any post-HCT NGS positivity resulted in an increase in relapse risk by multivariate analysis (hazard ratio, 7.7; P = .05). Absence of detectable IgH-V(D)J NGS-MRD pre-HCT defines good-risk patients potentially eligible for less intense treatment approaches. Post-HCT NGS-MRD is highly predictive of relapse and survival, suggesting a role for this technique in defining patients early who would be eligible for post-HCT interventions. The trial was registered at www.clinicaltrials.gov as #NCT00382109.

  19. Publication and dissemination of Annual Reports to shareholders in Nigeria and the United Kingdom: The Economics of Social Media and Electronic Communication

    OpenAIRE

    Olagoke Kuye

    2015-01-01

    While the cost of printing and sending out annual reports to shareholders by companies keep increasing and arguments have been made for cheaper and cost-effective ways of carrying out financial disclosure obligations by companies including electronic method, Companies’ Acts maintain that shareholders remain the ultimate decision-maker in the question whether electronic means of publishing annual reports is to be adopted as the primary means of publishing and disseminating annual reports among...

  20. MutAid: Sanger and NGS Based Integrated Pipeline for Mutation Identification, Validation and Annotation in Human Molecular Genetics.

    Science.gov (United States)

    Pandey, Ram Vinay; Pabinger, Stephan; Kriegner, Albert; Weinhäusel, Andreas

    2016-01-01

    Traditional Sanger sequencing as well as Next-Generation Sequencing have been used for the identification of disease causing mutations in human molecular research. The majority of currently available tools are developed for research and explorative purposes and often do not provide a complete, efficient, one-stop solution. As the focus of currently developed tools is mainly on NGS data analysis, no integrative solution for the analysis of Sanger data is provided and consequently a one-stop solution to analyze reads from both sequencing platforms is not available. We have therefore developed a new pipeline called MutAid to analyze and interpret raw sequencing data produced by Sanger or several NGS sequencing platforms. It performs format conversion, base calling, quality trimming, filtering, read mapping, variant calling, variant annotation and analysis of Sanger and NGS data under a single platform. It is capable of analyzing reads from multiple patients in a single run to create a list of potential disease causing base substitutions as well as insertions and deletions. MutAid has been developed for expert and non-expert users and supports four sequencing platforms including Sanger, Illumina, 454 and Ion Torrent. Furthermore, for NGS data analysis, five read mappers including BWA, TMAP, Bowtie, Bowtie2 and GSNAP and four variant callers including GATK-HaplotypeCaller, SAMTOOLS, Freebayes and VarScan2 pipelines are supported. MutAid is freely available at https://sourceforge.net/projects/mutaid.

  1. ngs (notochord granular surface) gene encodes a novel type of intermediate filament family protein essential for notochord maintenance in zebrafish.

    Science.gov (United States)

    Tong, Xiangjun; Xia, Zhidan; Zu, Yao; Telfer, Helena; Hu, Jing; Yu, Jingyi; Liu, Huan; Zhang, Quan; Sodmergen; Lin, Shuo; Zhang, Bo

    2013-01-25

    The notochord is an important organ involved in embryonic patterning and locomotion. In zebrafish, the mature notochord consists of a single stack of fully differentiated, large vacuolated cells called chordocytes, surrounded by a single layer of less differentiated notochordal epithelial cells called chordoblasts. Through genetic analysis of zebrafish lines carrying pseudo-typed retroviral insertions, a mutant exhibiting a defective notochord with a granular appearance was isolated, and the corresponding gene was identified as ngs (notochord granular surface), which was specifically expressed in the notochord. In the mutants, the notochord started to degenerate from 32 hours post-fertilization, and the chordocytes were then gradually replaced by smaller cells derived from chordoblasts. The granular notochord phenotype was alleviated by anesthetizing the mutant embryos with tricaine to prevent muscle contraction and locomotion. Phylogenetic analysis showed that ngs encodes a new type of intermediate filament (IF) family protein, which we named chordostatin based on its function. Under the transmission electron microcopy, bundles of 10-nm-thick IF-like filaments were enriched in the chordocytes of wild-type zebrafish embryos, whereas the chordocytes in ngs mutants lacked IF-like structures. Furthermore, chordostatin-enhanced GFP (EGFP) fusion protein assembled into a filamentous network specifically in chordocytes. Taken together, our work demonstrates that ngs encodes a novel type of IF protein and functions to maintain notochord integrity for larval development and locomotion. Our work sheds light on the mechanisms of notochord structural maintenance, as well as the evolution and biological function of IF family proteins.

  2. Corrosion survelliance for reactor materials in the calandria vault of pickering NGS a unit 1

    International Nuclear Information System (INIS)

    This paper presents selected results from an 18 month period of corrosion surveillance for structural materials inside the calandria vault of Pickering NGS Unit 'A'. Cooling pipe leaks have resulted in humidity build up inside the air-filled vault, and subsequent radiolysis of the water and air by the high gamma radiation field from the operating reactor results in the formation of nitric acid. Condensation of the nitric acid on cooler components (pipes, support brackets) promotes general corrosion and possibly localized corrosion of carbon steel structures. The corrosion surveillance system was installed to directly monitor changes in corrosion rates of selected materials. One period has been chosen in which, due to a bioshield cooling water leak, the corrosion rates increased

  3. Modeling of Pickering NGS B heat transport system seismic boundary leak tightness test

    International Nuclear Information System (INIS)

    In case of a seismic event in Pickering NGS B, Class IV and Class III power supplies may become unavailable. In an operating unit, such an event would results in reactor trip with the Heat Transport System (HTS) remaining at hot and pressurized conditions, while decay power is being removed by single-phase thermosyphoning. Under these circumstances, HTS pressure and inventory must be maintained within the seismically qualified portion of the system to prevent the breakdown of thermosyphoning. This paper presents the model employed to simulate the HTS pressure following the seismic event and the comparison between numerical results and actual data obtained with the recently developed P-079 test for HTS at cold and pressurized conditions. (author)

  4. Pickering NGS A: Assessment of calandria tube integrity following a sudden pressure tube failure

    International Nuclear Information System (INIS)

    The issue of calandria tube integrity following a sudden rupture of the pressure tube in Pickering NGS A reactor is addressed. Based on operating experience, only fish-mouth ruptures of the pressure tube are considered to be credible. The calandria tube response to the pressure tube break is delineated into three distinct stages, i.e. the initial transient response during the annulus filling stage, transient overpressurization and the final steady-state loading after bellows failure. The annulus response in the second stage is dominated by a waterhammer type overpressure transient with attenuation of this transient due to plastic straining of the calandria tube. The annulus pressure transients for various breaks and the sensitivity of the results to various parameters are presented. The strength margins of the calandria tube are evaluated to be relatively large. (author). 7 refs., 6 tabs., 6 figs

  5. Pickering NGS heat transport system decontamination using the CAN-DECON process

    International Nuclear Information System (INIS)

    In November 1981, a decontamination of the Pickering NGS Unit 1 heat transport system using the CAN-DECON process was carried out. The primary objective of the decontamination was to establish the effectiveness of the decontamination process in order to determine optimum tooling and manpower requirements for major dose-intensive reactor maintenance work. Laboratory scale development work suggested that the CAN-DECON process could, with modifications, produce decontamination factors (DFs) of 10 or better on carbon steel. The full scale decontamination, however, did not confirm these expectations. Radiation fields on the carbon steel headers and feeders were unchanged. Radiation fields on the Monel boilers which were low to start with were reduced by a factor of 1.5. This paper discusses the decontamination, its results, and the lessons learned from the decontamination

  6. Design and fabrication of the retube transfer cask for Bruce N.G.S

    International Nuclear Information System (INIS)

    The retubing of CANDU reactors is a complex process which involves the removal and disposal of highly activated and contaminated calandria tubes and pressure tubes. For Bruce 'A' N.G.S., old pressure tubes will be removed from the reactor by cutting them into three segments; two end fitting assemblies which measure up to 3.2 m in length, and one pressure tube segment which measures up to 6.3 m. in length. Calandria tubes are 6.2 m in length. The function of the retube transfer cask is to provide for shielded transfer of these components between the reactor face and the in-ground disposal facility. This paper describes the design and fabrication of this cask. (author) 1 tab., 7 figs

  7. Bruce NGS: A discharge channel flow simulation using MODTURC-CLAS

    International Nuclear Information System (INIS)

    Better understanding of the total residual chlorine (TRC) distribution and characteristics of water flow in the open discharge channel is desired to identify a representative sampling location for TRC in the cooling water discharge channel of a nuclear generating station. A three-dimensional flow simulation for Bruce NGS A was carried out using a state-of-the-art computer code MODTURC-CLAS. The results of this model are compared with data collected from a field measurement at Bruce A discharge channel in the summer of 1993. This model can be used to predict the characteristics of the discharge flows for various operating conditions of the station, and to help optimize the selection of a representative sampling point for TRC in the discharge channel. (author) 4 refs., 1 tab., 17 refs

  8. Evaluation of a pilot fish handling system at Bruce NGS 'A'

    International Nuclear Information System (INIS)

    A pilot fish recovery system using a Hidrostal fish pump was tested in the Bruce NGS 'A' forebay during June, 1984. Despite low forebay fish concentrations, the system was capable of capturing 97,000 alewife/day (3900 kg) if operated continuously. Post-pumping survival averaged 97%. It is estimated that a single pump could handle alewife runs in the 40,000 to 70,000 kg range, but multiple pumps or a single larger pump would be required to assure station protection from the largest runs (>100,000 kg). Results indicate that tank/trailer return of pumped fish is feasible, but other alternatives for returning fish to Lake Huron are also being considered

  9. 45 CFR 2400.2 - Annual competition.

    Science.gov (United States)

    2010-10-01

    ... 45 Public Welfare 4 2010-10-01 2010-10-01 false Annual competition. 2400.2 Section 2400.2 Public... FELLOWSHIP PROGRAM REQUIREMENTS General § 2400.2 Annual competition. To achieve its principal purposes, the Foundation holds an annual national competition to select teachers in grades 7-12, college seniors,...

  10. List of publications 1987

    International Nuclear Information System (INIS)

    This annual bibliography of publications from University of Oslo, Department of physics refers to research reports, contribution to newspapers and scientific journals, guest lectures, and popular science lectures

  11. Cancer modelling in the NGS era - Part I: Emerging technology and initial modelling.

    Science.gov (United States)

    Rovigatti, Ugo

    2015-11-01

    It is today indisputable that great progresses have been made in our molecular understanding of cancer cells, but an effective implementation of such knowledge into dramatic cancer-cures is still belated and yet desperately needed. This review gives a snapshot at where we stand today in this search for cancer understanding and definitive treatments, how far we have progressed and what are the major obstacles we will have to overcome both technologically and for disease modelling. In the first part, promising 3rd/4th Generation Sequencing Technologies will be summarized (particularly IonTorrent and OxfordNanopore technologies). Cancer modelling will be then reviewed from its origin in XIX Century Germany to today's NGS applications for cancer understanding and therapeutic interventions. Developments after Molecular Biology revolution (1953) are discussed as successions of three phases. The first, PH1, labelled "Clonal Outgrowth" (from 1960s to mid 1980s) was characterized by discoveries in cytogenetics (Nowell, Rowley) and viral oncology (Dulbecco, Bishop, Varmus), which demonstrated clonality. Treatments were consequently dominated by a "cytotoxic eradication" strategy with chemotherapeutic agents. In PH2, (from the mid 1980s to our days) the description of cancer as "Gene Networks" led to targeted-gene-therapies (TGTs). TGTs are the focus of Section 3: in view of their apparent failing (Ephemeral Therapies), alternative strategies will be discussed in review part II (particularly cancer immunotherapy, CIT). Additional Pitfalls impinge on the concepts of tumour heterogeneity (inter/intra; ITH). The described pitfalls set the basis for a new phase, PH3, which is called "NGS Era" and will be also discussed with ten emerging cancer models in the Review 2nd part.

  12. DEVELOPMENT OF ANALYTICAL METHODS FOR DETERMINING SUPPRESSOR CONCENTRATION IN THE MCU NEXT GENERATION SOLVENT (NGS)

    Energy Technology Data Exchange (ETDEWEB)

    Taylor-Pashow, K.; Fondeur, F.; White, T.; Diprete, D.; Milliken, C.

    2013-07-31

    Savannah River National Laboratory (SRNL) was tasked with identifying and developing at least one, but preferably two methods for quantifying the suppressor in the Next Generation Solvent (NGS) system. The suppressor is a guanidine derivative, N,N',N"-tris(3,7-dimethyloctyl)guanidine (TiDG). A list of 10 possible methods was generated, and screening experiments were performed for 8 of the 10 methods. After completion of the screening experiments, the non-aqueous acid-base titration was determined to be the most promising, and was selected for further development as the primary method. {sup 1}H NMR also showed promising results from the screening experiments, and this method was selected for further development as the secondary method. Other methods, including {sup 36}Cl radiocounting and ion chromatography, also showed promise; however, due to the similarity to the primary method (titration) and the inability to differentiate between TiDG and TOA (tri-n-ocytlamine) in the blended solvent, {sup 1}H NMR was selected over these methods. Analysis of radioactive samples obtained from real waste ESS (extraction, scrub, strip) testing using the titration method showed good results. Based on these results, the titration method was selected as the method of choice for TiDG measurement. {sup 1}H NMR has been selected as the secondary (back-up) method, and additional work is planned to further develop this method and to verify the method using radioactive samples. Procedures for analyzing radioactive samples of both pure NGS and blended solvent were developed and issued for the both methods.

  13. HapTree: a novel Bayesian framework for single individual polyplotyping using NGS data.

    Directory of Open Access Journals (Sweden)

    Emily Berger

    2014-03-01

    Full Text Available As the more recent next-generation sequencing (NGS technologies provide longer read sequences, the use of sequencing datasets for complete haplotype phasing is fast becoming a reality, allowing haplotype reconstruction of a single sequenced genome. Nearly all previous haplotype reconstruction studies have focused on diploid genomes and are rarely scalable to genomes with higher ploidy. Yet computational investigations into polyploid genomes carry great importance, impacting plant, yeast and fish genomics, as well as the studies of the evolution of modern-day eukaryotes and (epigenetic interactions between copies of genes. In this paper, we describe a novel maximum-likelihood estimation framework, HapTree, for polyploid haplotype assembly of an individual genome using NGS read datasets. We evaluate the performance of HapTree on simulated polyploid sequencing read data modeled after Illumina sequencing technologies. For triploid and higher ploidy genomes, we demonstrate that HapTree substantially improves haplotype assembly accuracy and efficiency over the state-of-the-art; moreover, HapTree is the first scalable polyplotyping method for higher ploidy. As a proof of concept, we also test our method on real sequencing data from NA12878 (1000 Genomes Project and evaluate the quality of assembled haplotypes with respect to trio-based diplotype annotation as the ground truth. The results indicate that HapTree significantly improves the switch accuracy within phased haplotype blocks as compared to existing haplotype assembly methods, while producing comparable minimum error correction (MEC values. A summary of this paper appears in the proceedings of the RECOMB 2014 conference, April 2-5.

  14. Determining performance characteristics of an NGS-based HLA typing method for clinical applications.

    Science.gov (United States)

    Duke, J L; Lind, C; Mackiewicz, K; Ferriola, D; Papazoglou, A; Gasiewski, A; Heron, S; Huynh, A; McLaughlin, L; Rogers, M; Slavich, L; Walker, R; Monos, D S

    2016-03-01

    This study presents performance specifications of an in-house developed human leukocyte antigen (HLA) typing assay using next-generation sequencing (NGS) on the Illumina MiSeq platform. A total of 253 samples, previously characterized for HLA-A, -B, -C, -DRB1 and -DQB1 were included in this study, which were typed at high-resolution using a combination of Sanger sequencing, sequence-specific primer (SSP) and sequence-specific oligonucleotide probe (SSOP) technologies and recorded at the two-field level. Samples were selected with alleles that cover a high percentage of HLA specificities in each of five different race/ethnic groups: European, African-American, Asian Pacific Islander, Hispanic and Native American. Sequencing data were analyzed by two software programs, Omixon's target and GenDx's NGSengine. A number of metrics including allele balance, sensitivity, specificity, precision, accuracy and remaining ambiguity were assessed. Data analyzed by the two software systems are shown independently. The majority of alleles were identical in the exonic sequences (third field) with both programs for HLA-A, -B, -C and -DQB1 in 97.7% of allele determinations. Among the remaining discrepant genotype calls at least one of the analysis programs agreed with the reference typing. Upon additional manual analysis 100% of the 2530 alleles were concordant with the reference HLA genotypes; the remaining ambiguities did not exceed 0.8%. The results demonstrate the feasibility and significant benefit of HLA typing by NGS as this technology is highly accurate, eliminates virtually all ambiguities, provides complete sequencing information for the length of the HLA gene and forms the basis for utilizing a single methodology for HLA typing in the immunogenetics labs.

  15. Genotype-phenotype correlation in a cohort of Portuguese patients comprising the entire spectrum of VWD types: impact of NGS.

    Science.gov (United States)

    Fidalgo, Teresa; Salvado, Ramon; Corrales, Irene; Pinto, Silva Catarina; Borràs, Nina; Oliveira, Ana; Martinho, Patricia; Ferreira, Gisela; Almeida, Helena; Oliveira, Cristina; Marques, Dalila; Gonçalves, Elsa; Diniz, MJoão; Antunes, Margarida; Tavares, Alice; Caetano, Gonçalo; Kjöllerström, Paula; Maia, Raquel; Sevivas, Teresa S; Vidal, Francisco; Ribeiro, Leticia

    2016-07-01

    The diagnosis of von Willebrand disease (VWD), the most common inherited bleeding disorder, is characterised by a variable bleeding tendency and heterogeneous laboratory phenotype. The sequencing of the entire VWF coding region has not yet become a routine practice in diagnostic laboratories owing to its high costs. Nevertheless, next-generation sequencing (NGS) has emerged as an alternative to overcome this limitation. We aimed to determine the correlation of genotype and phenotype in 92 Portuguese individuals from 60 unrelated families with VWD; therefore, we directly sequenced VWF. We compared the classical Sanger sequencing approach and NGS to assess the value-added effect on the analysis of the mutation distribution in different types of VWD. Sixty-two different VWF mutations were identified, 27 of which had not been previously described. NGS detected 26 additional mutations, contributing to a broad overview of the mutant alleles present in each VWD type. Twenty-nine probands (48.3 %) had two or more mutations; in addition, mutations with pleiotropic effects were detected, and NGS allowed an appropriate classification for seven of them. Furthermore, the differential diagnosis between VWD 2B and platelet type VWD (n = 1), Bernard-Soulier syndrome and VWD 2B (n = 1), and mild haemophilia A and VWD 2N (n = 2) was possible. NGS provided an efficient laboratory workflow for analysing VWF. These findings in our cohort of Portuguese patients support the proposal that improving VWD diagnosis strategies will enhance clinical and laboratory approaches, allowing to establish the most appropriate treatment for each patient.

  16. American Public Health Association

    Science.gov (United States)

    About APHA Join Renew Annual Meeting Careers Contact Us Store LOGIN What is Public Health? Generation Public Health Creating Healthy Communities Topics & Issues Gun Violence Climate Change Environmental Health Health Equity Health Reform Tobacco Vaccines ...

  17. NGS-200型GPS测量系统在建立三维控制网中的应用%Application of NGS-200 GPS in Establishment of Three-Dimensional Control Network

    Institute of Scientific and Technical Information of China (English)

    赵俊兰; 冯建秋

    2001-01-01

    介绍了NGS-200GPS测量系统的组成及原理,阐述了利用该系统在北京八大处公园建立测区首级平面控制网的具体做法,对布网方案、数据处理、平差计算和测量结果可靠性进行了分析.实践表明NGS-200GPS在八大处公园山地地形复杂,树木覆盖较密的情况下 ,其性能仍然比较稳定,所取得的测量结果达到了国家有关规范要求.该系统替代常规测量技术是经济、合理可靠的.

  18. Proceedings of the Annual Meeting of the Association for Education in Journalism and Mass Communication (81st, Baltimore, Maryland, August 5-8, 1988). Public Relations.

    Science.gov (United States)

    Association for Education in Journalism and Mass Communication.

    The Public Relations section of the Proceedings contains the following 15 papers: "The World Wide Web as a Public Relations Medium: The Use of Research, Planning, and Evaluation by Web Site Decision-Makers" (Candace White and Niranjan Raman); "Enlightened Self Interest--An Ethical Baseline for Teaching Corporate Public Relations" (Patricia T.…

  19. e-DNA meta-barcoding: from NGS raw data to taxonomic profiling.

    Science.gov (United States)

    Bruno, Fosso; Marinella, Marzano; Santamaria, Monica

    2015-01-01

    In recent years, thanks to the essential support provided by the Next-Generation Sequencing (NGS) technologies, Metagenomics is enabling the direct access to the taxonomic and functional composition of mixed microbial communities living in any environmental niche, without the prerequisite to isolate or culture the single organisms. This approach has already been successfully applied for the analysis of many habitats, such as water or soil natural environments, also characterized by extreme physical and chemical conditions, food supply chains, and animal organisms, including humans. A shotgun sequencing approach can lead to investigate both organisms and genes diversity. Anyway, if the purpose is limited to explore the taxonomic complexity, an amplicon-based approach, based on PCR-targeted sequencing of selected genetic species markers, commonly named "meta-barcodes", is desirable. Among the genomic regions most widely used for the discrimination of bacterial organisms, in some cases up to the species level, some hypervariable domains of the gene coding for the 16S rRNA occupy a prominent place. The amplification of a certain meta-barcode from a microbial community through the use of PCR primers able to work in the entire considered taxonomic group is the first task after the extraction of the total DNA. Generally, this step is followed by the high-throughput sequencing of the resulting amplicons libraries by means of a selected NGS platform. Finally, the interpretation of the huge amount of produced data requires appropriate bioinformatics tools and know-how in addition to efficient computational resources. Here a computational methodology suitable for the taxonomic characterization of 454 meta-barcode sequences is described in detail. In particular, a dataset covering the V1-V3 region belonging to the bacterial 16S rRNA coding gene and produced in the Human Microbiome Project (HMP) from a palatine tonsils sample is analyzed. The proposed exercise includes the

  20. e-DNA meta-barcoding: from NGS raw data to taxonomic profiling.

    Science.gov (United States)

    Bruno, Fosso; Marinella, Marzano; Santamaria, Monica

    2015-01-01

    In recent years, thanks to the essential support provided by the Next-Generation Sequencing (NGS) technologies, Metagenomics is enabling the direct access to the taxonomic and functional composition of mixed microbial communities living in any environmental niche, without the prerequisite to isolate or culture the single organisms. This approach has already been successfully applied for the analysis of many habitats, such as water or soil natural environments, also characterized by extreme physical and chemical conditions, food supply chains, and animal organisms, including humans. A shotgun sequencing approach can lead to investigate both organisms and genes diversity. Anyway, if the purpose is limited to explore the taxonomic complexity, an amplicon-based approach, based on PCR-targeted sequencing of selected genetic species markers, commonly named "meta-barcodes", is desirable. Among the genomic regions most widely used for the discrimination of bacterial organisms, in some cases up to the species level, some hypervariable domains of the gene coding for the 16S rRNA occupy a prominent place. The amplification of a certain meta-barcode from a microbial community through the use of PCR primers able to work in the entire considered taxonomic group is the first task after the extraction of the total DNA. Generally, this step is followed by the high-throughput sequencing of the resulting amplicons libraries by means of a selected NGS platform. Finally, the interpretation of the huge amount of produced data requires appropriate bioinformatics tools and know-how in addition to efficient computational resources. Here a computational methodology suitable for the taxonomic characterization of 454 meta-barcode sequences is described in detail. In particular, a dataset covering the V1-V3 region belonging to the bacterial 16S rRNA coding gene and produced in the Human Microbiome Project (HMP) from a palatine tonsils sample is analyzed. The proposed exercise includes the

  1. Photometric calibration of NGS/POSS and ESO/SRC plates using the NOAO PDS measuring engine. II - Surface photometry

    Science.gov (United States)

    Cutri, Roc M.; Low, Frank J.; Guhathakurta, Puragra

    1993-01-01

    In this paper we present a method to calibrate surface photometry of faint sources measured from direct photographic plates, such as those of the NGS/POSS and ESO/SRC Sky Survey. This calibration procedure does not require scanning sensitometer spots on the plates, but instead uses measurements of the brightness profiles of many faint stars of known brightness to fit a linearized approximation to the characteristic curve. The approximation is valid for only low- to medium-density emulsions, so this technique is appropriate only for relatively faint emission. Comparison between measurements of representative extended sources on the NGS/POSS and CCD images indicates that surface photometry can be obtained from the Sky Survey plates accurate to 0.1-0.3 mag in the range mu(B) between 23 and 27 and mu(R) between 22 and 26 mag/sq arcsec.

  2. Practical application of self-organizing maps to interrelate biodiversity and functional data in NGS-based metagenomics

    OpenAIRE

    Weber, Marc; Teeling, Hanno; Huang, Sixing; Waldmann, Jost; Kassabgy, Mariette; Fuchs, Bernhard M.; Klindworth, Anna; Klockow, Christine; Wichels, Antje; Gerdts, Gunnar; Amann, Rudolf; Glöckner, Frank Oliver

    2010-01-01

    Next-generation sequencing (NGS) technologies have enabled the application of broad-scale sequencing in microbial biodiversity and metagenome studies. Biodiversity is usually targeted by classifying 16S ribosomal RNA genes, while metagenomic approaches target metabolic genes. However, both approaches remain isolated, as long as the taxonomic and functional information cannot be interrelated. Techniques like self-organizing maps (SOMs) have been applied to cluster metagenomes into taxon-specif...

  3. lociNGS: A Lightweight Alternative for Assessing Suitability of Next-Generation Loci for Evolutionary Analysis

    OpenAIRE

    Hird, Sarah M.

    2012-01-01

    Genomic enrichment methods and next-generation sequencing produce uneven coverage for the portions of the genome (the loci) they target; this information is essential for ascertaining the suitability of each locus for further analysis. lociNGS is a user-friendly accessory program that takes multi-FASTA formatted loci, next-generation sequence alignments and demographic data as input and collates, displays and outputs information about the data. Summary information includes the parameters cove...

  4. Annual Report 2000

    International Nuclear Information System (INIS)

    This annual report gives the activities during the year 2000 and information about the General Direction of the Energy and Raw Materials (DGEMP) in France. The highlights of the year are discussed in the main following topics: public utilities, the Erika oil slick, the petroleum products prices increase, the energy efficiency national program, Eole 2005, the nuclear industry management. The french energy accounting, the publications and the organization of the DGEMP are also provided. (A.L.B.)

  5. An NGS-Independent Strategy for Proteome-Wide Identification of Single Amino Acid Polymorphisms by Mass Spectrometry.

    Science.gov (United States)

    Xiong, Yun; Guo, Yufeng; Xiao, Weidi; Cao, Qichen; Li, Shanshan; Qi, Xianni; Zhang, Zhidan; Wang, Qinhong; Shui, Wenqing

    2016-03-01

    Detection of proteins containing single amino acid polymorphisms (SAPs) encoded by nonsynonymous SNPs (nsSNPs) can aid researchers in studying the functional significance of protein variants. Most proteogenomic approaches for large-scale SAPs mapping require construction of a sample-specific database containing protein variants predicted from the next-generation sequencing (NGS) data. Searching shotgun proteomic data sets against these NGS-derived databases allowed for identification of SAP peptides, thus validating the proteome-level sequence variation. Contrary to the conventional approaches, our study presents a novel strategy for proteome-wide SAP detection without relying on sample-specific NGS data. By searching a deep-coverage proteomic data set from an industrial thermotolerant yeast strain using our strategy, we identified 337 putative SAPs compared to the reference genome. Among the SAP peptides identified with stringent criteria, 85.2% of SAP sites were validated using whole-genome sequencing data obtained for this organism, which indicates high accuracy of SAP identification with our strategy. More interestingly, for certain SAP peptides that cannot be predicted by genomic sequencing, we used synthetic peptide standards to verify expression of peptide variants in the proteome. Our study has provided a unique tool for proteogenomics to enable proteome-wide direct SAP identification and capture nongenetic protein variants not linked to nsSNPs.

  6. Intra-individual polymorphism in chloroplasts from NGS data: where does it come from and how to handle it?

    Science.gov (United States)

    Scarcelli, N; Mariac, C; Couvreur, T L P; Faye, A; Richard, D; Sabot, F; Berthouly-Salazar, C; Vigouroux, Y

    2016-03-01

    Next-generation sequencing allows access to a large quantity of genomic data. In plants, several studies used whole chloroplast genome sequences for inferring phylogeography or phylogeny. Even though the chloroplast is a haploid organelle, NGS plastome data identified a nonnegligible number of intra-individual polymorphic SNPs. Such observations could have several causes such as sequencing errors, the presence of heteroplasmy or transfer of chloroplast sequences in the nuclear and mitochondrial genomes. The occurrence of allelic diversity has practical important impacts on the identification of diversity, the analysis of the chloroplast data and beyond that, significant evolutionary questions. In this study, we show that the observed intra-individual polymorphism of chloroplast sequence data is probably the result of plastid DNA transferred into the mitochondrial and/or the nuclear genomes. We further assess nine different bioinformatics pipelines' error rates for SNP and genotypes calling using SNPs identified in Sanger sequencing. Specific pipelines are adequate to deal with this issue, optimizing both specificity and sensitivity. Our results will allow a proper use of whole chloroplast NGS sequence and will allow a better handling of NGS chloroplast sequence diversity.

  7. RNA-CODE: a noncoding RNA classification tool for short reads in NGS data lacking reference genomes.

    Directory of Open Access Journals (Sweden)

    Cheng Yuan

    Full Text Available The number of transcriptomic sequencing projects of various non-model organisms is still accumulating rapidly. As non-coding RNAs (ncRNAs are highly abundant in living organism and play important roles in many biological processes, identifying fragmentary members of ncRNAs in small RNA-seq data is an important step in post-NGS analysis. However, the state-of-the-art ncRNA search tools are not optimized for next-generation sequencing (NGS data, especially for very short reads. In this work, we propose and implement a comprehensive ncRNA classification tool (RNA-CODE for very short reads. RNA-CODE is specifically designed for ncRNA identification in NGS data that lack quality reference genomes. Given a set of short reads, our tool classifies the reads into different types of ncRNA families. The classification results can be used to quantify the expression levels of different types of ncRNAs in RNA-seq data and ncRNA composition profiles in metagenomic data, respectively. The experimental results of applying RNA-CODE to RNA-seq of Arabidopsis and a metagenomic data set sampled from human guts demonstrate that RNA-CODE competes favorably in both sensitivity and specificity with other tools. The source codes of RNA-CODE can be downloaded at http://www.cse.msu.edu/~chengy/RNA_CODE.

  8. A new method to prevent carry-over contaminations in two-step PCR NGS library preparations.

    Science.gov (United States)

    Seitz, Volkhard; Schaper, Sigrid; Dröge, Anja; Lenze, Dido; Hummel, Michael; Hennig, Steffen

    2015-11-16

    Two-step PCR procedures are an efficient and well established way to generate amplicon libraries for NGS sequencing. However, there is a high risk of cross-contamination by carry-over of amplicons from first to second amplification rounds, potentially leading to severe misinterpretation of results. Here we describe a new method able to prevent and/or to identify carry-over contaminations by introducing the K-box, a series of three synergistically acting short sequence elements. Our K-boxes are composed of (i) K1 sequences for suppression of contaminations, (ii) K2 sequences for detection of possible residual contaminations and (iii) S sequences acting as separators to avoid amplification bias. In order to demonstrate the effectiveness of our method we analyzed two-step PCR NGS libraries derived from a multiplex PCR system for detection of T-cell receptor beta gene rearrangements. We used this system since it is of high clinical relevance and may be affected by very low amounts of contaminations. Spike-in contaminations are effectively blocked by the K-box even at high rates as demonstrated by ultra-deep sequencing of the amplicons. Thus, we recommend implementation of the K-box in two-step PCR-based NGS systems for research and diagnostic applications demanding high sensitivity and accuracy.

  9. AG-NGS: a powerful and user-friendly computing application for the semi-automated preparation of next-generation sequencing libraries using open liquid handling platforms.

    Science.gov (United States)

    Callejas, Sergio; Álvarez, Rebeca; Benguria, Alberto; Dopazo, Ana

    2014-01-01

    Next-generation sequencing (NGS) is becoming one of the most widely used technologies in the field of genomics. Library preparation is one of the most critical, hands-on, and time-consuming steps in the NGS workflow. Each library must be prepared in an independent well, increasing the number of hours required for a sequencing run and the risk of human-introduced error. Automation of library preparation is the best option to avoid these problems. With this in mind, we have developed automatic genomics NGS (AG-NGS), a computing application that allows an open liquid handling platform to be transformed into a library preparation station without losing the potential of an open platform. Implementation of AG-NGS does not require programming experience, and the application has also been designed to minimize implementation costs. Automated library preparation with AG-NGS generated high-quality libraries from different samples, demonstrating its efficiency, and all quality control parameters fell within the range of optimal values.

  10. Vibration analysis of the PHT piping system of Point Lepreau NGS

    International Nuclear Information System (INIS)

    The Point Lepreau NGS is a 600 MeW CANDU Unit and has four Primary Heat Transport (PHT) pumps. In the course of a specific mode of operation with only two pumps running a certain amount of heavy water flows through a stability line, which has two orifice plates. During that mode of operation the PHT piping experienced a level of vibration high enough to discourage the two pump mode of operation. This paper presents the course of actions performed to establish a vibration mechanism. An extensive test program to formulate the nature of vibration and find out characteristics of the system has been implemented. Vibration accelerations, pressure pulsations, flow rates, system pressures and operating temperatures have been recorded. An impact test for structural natural frequencies was utilized. All possible solutions to remedy the problem have been discussed. The most practical solution to the PHT piping vibration problem is recommended. The design criterion, for avoiding a severe vibration caused by the vortex shedding at an orifice in the stability line of a 600 MeW Unit, is derived

  11. Improving flux tilt control while adjuster control rods are removed from the Pickering NGS A reactor

    International Nuclear Information System (INIS)

    Removal of adjuster control rods from the Pickering NGS A reactor core results in flux peaking and higher fuel powers in the centre region of the core. The present flux tilt control algorithm increases the level of the light water neutron absorber in the centre liquid zone controllers in an attempt to nullify flux peaking. However, due to the limited depth of the neutron absorption capability of the liquid zone controllers, the pre-removal zone powers can not be achieved. This results in saturation of liquid zone controller levels and reduced flux tilt control. Recent operating experience as shown that in certain situations the reduced flux tilt control capability with adjusters removed results in uncorrected side to side azimuthal flux tilts. To increase tilt control in these situations an improved flux tilt control algorithm has been developed which switches the zone power flux tilt control targets to more realistic obtainable values as adjusters are removed. In this paper the computer simulations and analysis performed to develop and test the improved flux tilt algorithm is described. Also the improved performance of the new algorithm in one event will be demonstrated. 2 refs., 9 figs

  12. Analysis of the pressure tube failure at Pickering NGS A unit 2

    International Nuclear Information System (INIS)

    About noon on the 1st August 1983, the pressure tube in fuel channel G16 of the Pickering NGS A unit 2 reactor developed a critical through-wall crack and failed by fast fracture after 342 days of continuous full power operation. Following removal of the fuel, a TV inspection inside the fuel channel revealed an axial crack in the bottom of the pressure tube approximately 2 metres long, in which two fuel pencils were lodged. After extracting the fuel pencils, the fuel channel was removed and shipped to the Atomic Energy of Canada Limited's Chalk River Nuclear Laboratories for detailed examination to determine the cause of failure. Examination of failures normally takes a course of looking at the fracture and gradually refining the work into finer detail to determine the actual origin of the failure. In this case, several other aspects also needed to be examined. The position of the garter spring was very important, as was examination of the calandria tube, which was subsequently removed. During the inspection several other fuel channels in Pickering A, Bruce A and NPD reactors were inspected and some removed for further assessment at CRNL. All these aspects came together to outline the cause and mechanism of failure. The following gives a very brief review of the salient features of the examination of Zircaloy 2 and zirconium-niobium pressure tubes and the implication for operation of subsequent reactors which have zirconium-niobium pressure tubes

  13. Scientific annual report 1980 DESY

    International Nuclear Information System (INIS)

    This annual report contains a survey about the organization of DESY, a description of the different experiments performed at DESY and the developments in accelerator technology and data processing, as well as a list of publications and speeches. (HSI)

  14. Annual Report 2000

    International Nuclear Information System (INIS)

    The Institut Laue-Langevin (ILL) is an international research center using neutrons to probe the microscopic structure and dynamics of a broad range of materials. This annual report presents the ILL activities in 2000: the scientific highlights, the Millennium programme and the new developments, the workshops organized by the ILL, the experimental programme and the publications. (A.L.B.)

  15. Environment annual report 1993

    International Nuclear Information System (INIS)

    In the 1993 Environment Annual Report for BNFL, data are presented for radioactive discharges to the environment and their associated doses to the criteria group members of the public in the vicinity of Sellafield, Drigg, Chapelcross, Springfields and Capenhurst. Similarly, data are also presented for non-radioactive discharges to water and air for each site. (UK)

  16. Annual report 1982. Annex

    International Nuclear Information System (INIS)

    This is the scientific annual report 1982 of the ILL on R and D activities of the different colleges: fundamental and nuclear physics, inelastic scattering in simple solids, crystallographic and magnetic structures, fluids and amorphous substances, imperfections, biology, chemistry, theory. Abstracts of the publications published during the year are presented

  17. Annual report 1981

    International Nuclear Information System (INIS)

    This annual report contains short descriptions of the work done at the named institute. These concern the study of hyperfine interactions, experiments with synchroton radiation, experiments at storage rings, and experiments at CERN. Furthermore, a list of publications, contributions to conferences and these is presented. (HSI)

  18. Annual Report 2000

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2000-07-01

    The Institut Laue-Langevin (ILL) is an international research center using neutrons to probe the microscopic structure and dynamics of a broad range of materials. This annual report presents the ILL activities in 2000: the scientific highlights, the Millennium programme and the new developments, the workshops organized by the ILL, the experimental programme and the publications. (A.L.B.)

  19. HASYLAB annual report 1987

    International Nuclear Information System (INIS)

    This annual report contains extended abstracts about the work performed at HASYLAB together with a list of publications, speeches, and theses. The work concerns technical developments, the study of the electronic structure of atoms, molecules, solids, surfaces, and liquids, X-ray structure studies of solids and interfaces, molecular biology, further applications of synchrotron radiation, and experimental developments. (HSI)

  20. NIKHEF Annual Report 1982

    International Nuclear Information System (INIS)

    In this annual report 1982, the NIKHEF research programs of high-energy physics, nuclear physics and radiochemistry is described in a wide context. Next, the reports of the individual projects of section-H and section-K are described in detail. Finally, the report gives some statistical information of publications, colloquia and co-workers. (Auth.)

  1. TIARA annual report 2004

    International Nuclear Information System (INIS)

    This annual report describes research and development activities which have been performed with the TIARA (Takasaki Ion Accelerators for Advanced Radiation Application) facilities from April 1, 2004 to March 31, 2005. The 126 of the presented papers are indexed individually. A list of publications, the type of research collaborations and organization of TIARA are also given as appendices. (J.P.N.)

  2. Comparison of targeted next-generation sequencing (NGS) and real-time PCR in the detection of EGFR, KRAS, and BRAF mutations on formalin-fixed, paraffin-embedded tumor material of non-small cell lung carcinoma-superiority of NGS.

    Science.gov (United States)

    Tuononen, Katja; Mäki-Nevala, Satu; Sarhadi, Virinder Kaur; Wirtanen, Aino; Rönty, Mikko; Salmenkivi, Kaisa; Andrews, Jenny M; Telaranta-Keerie, Aino I; Hannula, Sari; Lagström, Sonja; Ellonen, Pekka; Knuuttila, Aija; Knuutila, Sakari

    2013-05-01

    The development of tyrosine kinase inhibitor treatments has made it important to test cancer patients for clinically significant gene mutations that influence the benefit of treatment. Targeted next-generation sequencing (NGS) provides a promising method for diagnostic purposes by enabling the simultaneous detection of multiple mutations in various genes in a single test. The aim of our study was to screen EGFR, KRAS, and BRAF mutations by targeted NGS and commonly used real-time polymerase chain reaction (PCR) methods to evaluate the feasibility of targeted NGS for the detection of the mutations. Furthermore, we aimed to identify potential novel mutations by targeted NGS. We analyzed formalin-fixed, paraffin-embedded (FFPE) tumor tissue specimens from 81 non-small cell lung carcinoma patients. We observed a significant concordance (from 96.3 to 100%) of the EGFR, KRAS, and BRAF mutation detection results between targeted NGS and real-time PCR. Moreover, targeted NGS revealed seven nonsynonymous single-nucleotide variations and one insertion-deletion variation in EGFR not detectable by the real-time PCR methods. The potential clinical significance of these variants requires elucidation in future studies. Our results support the use of targeted NGS in the screening of EGFR, KRAS, and BRAF mutations in FFPE tissue material.

  3. Bavarian Land Office for Environmental Protection. 1996 annual report and abstracts of publications; Bayerisches Landesamt fuer Umweltschutz. Taetigkeitsbericht 1996 und Kurzbeitraege

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-12-31

    The annual report informs about the activities of the Bavarian Land Office for Environmental Protection in the following fields: clean air policy, waste management, management of soil pollution and remedial action, nuclear energy and radiation protection, nature conservation and landscape conservation, LfU laboratory, environmental hygiene, and data processing (SR) [Deutsch] Der vorliegende Bericht beschreibt die Taetigkeiten des Bayerischen Landesamtes fuer Umweltschutz auf folgenden Gebieten: Immissionsschutz, Abfallwirtschaft, Altlasten und Bodenschutz, Kernenergie und Strahlenschutz, Naturschutz und Landschaftspflege, Zentrallabors und Umwelthygiene, sowie Datenverarbeitung. (SR)

  4. Petroleum marketing annual 1994

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-08-24

    The Petroleum Marketing Annual (PMA) provides information and statistical data on a variety of crude oils and refined petroleum products. The publication presents statistics on crude oil costs and refined petroleum products sales for use by industry, government, private sector analysis, educational institutions, and consumers. Data on crude oil include the domestic first purchase price, the fob and landed cost of imported crude oil, and the refiners` acquisition cost of crude oil. Refined petroleum product sales data include motor gasoline, distillates, residuals, aviation fuels, kerosene, and propane. The Petroleum Marketing Division, Office of Oil and Gas, Energy Information Administration ensures the accuracy, quality, and confidentiality of the published data in the Petroleum Marketing Annual. For this production, all estimates have been recalculated since their earlier publication in the Petroleum Marketing Monthly (PMM). These calculations made use of additional data and corrections that were received after the PMM publication date.

  5. Petroleum marketing annual 1994

    International Nuclear Information System (INIS)

    The Petroleum Marketing Annual (PMA) provides information and statistical data on a variety of crude oils and refined petroleum products. The publication presents statistics on crude oil costs and refined petroleum products sales for use by industry, government, private sector analysis, educational institutions, and consumers. Data on crude oil include the domestic first purchase price, the fob and landed cost of imported crude oil, and the refiners' acquisition cost of crude oil. Refined petroleum product sales data include motor gasoline, distillates, residuals, aviation fuels, kerosene, and propane. The Petroleum Marketing Division, Office of Oil and Gas, Energy Information Administration ensures the accuracy, quality, and confidentiality of the published data in the Petroleum Marketing Annual. For this production, all estimates have been recalculated since their earlier publication in the Petroleum Marketing Monthly (PMM). These calculations made use of additional data and corrections that were received after the PMM publication date

  6. Papers of the Canadian Institute's 3. annual conference on public relations for the energy industry : best practices for communication in volatile energy markets

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2004-07-01

    In recent years, the energy industry has been subjected to intense public scrutiny and has faced difficult years that have had a damaging effect on its reputation. The industry is now faced with the challenge of rebuilding public confidence and trust. This conference provides some of the tools needed for communications departments to succeed in rebuilding the image of the industry. The topics of discussion ranged from managing public perception, communication challenges regarding high energy prices, joint ventures, and social responsibility regarding environmental impacts. Strategies to prevent crisis situations were also described. The conference featured 13 presentations, of which 5 have been indexed separately for inclusion in this database. refs., tabs., figs.

  7. Next Generation Solvent (NGS): Development for Caustic-Side Solvent Extraction of Cesium

    International Nuclear Information System (INIS)

    This report summarizes the FY 2010 and 2011 accomplishments at Oak Ridge National Laboratory (ORNL) in developing the Next Generation Caustic-Side Solvent Extraction (NG-CSSX) process, referred to commonly as the Next Generation Solvent (NGS), under funding from the U.S. Department of Energy, Office of Environmental Management (DOE-EM), Office of Technology Innovation and Development. The primary product of this effort is a process solvent and preliminary flowsheet capable of meeting a target decontamination factor (DF) of 40,000 for worst-case Savannah River Site (SRS) waste with a concentration factor of 15 or higher in the 18-stage equipment configuration of the SRS Modular Caustic-Side Solvent Extraction Unit (MCU). In addition, the NG-CSSX process may be readily adapted for use in the SRS Salt Waste Processing Facility (SWPF) or in supplemental tank-waste treatment at Hanford upon appropriate solvent or flowsheet modifications. Efforts in FY 2010 focused on developing a solvent composition and process flowsheet for MCU implementation. In FY 2011 accomplishments at ORNL involved a wide array of chemical-development activities and testing up through single-stage hydraulic and mass-transfer tests in 5-cm centrifugal contactors. Under subcontract from ORNL, Argonne National Laboratory (ANL) designed a preliminary flowsheet using ORNL cesium distribution data, and Tennessee Technological University confirmed a chemical model for cesium distribution ratios (DCs) as a function of feed composition. Interlaboratory efforts were coordinated with complementary engineering tests carried out (and reported separately) by personnel at Savannah River National Laboratory (SRNL) and Savannah River Remediation (SRR) with helpful advice by Parsons Engineering and General Atomics on aspects of possible SWPF implementation.

  8. Expected reactivity effect of fuel channel coolant boiling in the Darlington NGS A reactor core

    International Nuclear Information System (INIS)

    We have developed a formalism for estimating the expected reactivity due to channel boiling in any reactor designed to have some quality in the channel. In applying this formalism to the Darlington NGS A equilibrium core, we calculate a value of 0.024 ± 0.003 mk at 100% power operation. In Darlington, the channel feeders are individually sized so that the coolant in each channel has some boiling on reaching the entrance to the reactor outlet header. (Hereafter called the 'ROH quality'). The design is such that when each channel is at its nominal time-averaged 100 percent power, the quality at the ROH should be just under 2%. The day-to-day variation of each channel's power around its time-averaged value (i.e., 'ripple') results in a corresponding variation in the quality and consequently in the reactivity due to boiling. Traditionally, fuel management codes such as SORO, FMDP, RFSP and OHRFSP use fuel properties generated by a lattice code such as POWDERPUFS or LATREP. These fuel properties are functions of fuel irradiation only, with all other core-varying input parameters to the lattice code held constant at core-averaged values. Recently, some work has gone into developing a Pt. Lepreau version of RFSP in which the fuel properties are functions of fuel temperature and coolant density as well as of fuel irradiation. This paper reports the results of a study which was undertaken to quantify the expected variation in core reactivity due to this day-to-day variation in channel power and channel boiling. It could then be determined whether the reactivity effect of this boiling is sufficient to justify the explicit representation of the fuel properties as a function of coolant density

  9. Oxygen addition to the annulus gas systems of Pickering NGS A, Units 3 and 4

    International Nuclear Information System (INIS)

    An Oxygen (O2) addition system has been designed and installed in conjunction with the Annulus Gas System (AGS) of Pickering NGS a, Unit 3. It has been operational since January 1989. One of the short term purposes of O2 addition is to remove Carbon-14 (C-14) deposits on pressure tube (PT) surfaces through oxidation to carbon (14) dioxide which is subsequently removed by absorption/chemical reaction in a calcium hydroxide bed. Removal of C-14 deposits is necessary to minimize the occupational dose to workers during retubing. Experience to date has shown satisfactory operation of the O2 addition system for Unit 3 AGS, which has provided controlled O2 concentrations up to 10 percent (V/V). The O2 bottle supply system has involved significant handling requirements by the station. This will be minimized for Unit 4 where a liquid O2 tank has been selected for the supply. The Unit 4 O2 addition system is otherwise similar to that for Unit 3 except that lower O2 addition rates are accommodated. This reflects the fact that O2 addition for Unit 3 is designed for purge operation only whereas that for Unit 4 is designed for both purge recirculation operation. The latter mode is required for maintaining an impervious oxide layer on the surface of the PTs when satisfactory C-14 decontamination has been achieved. It is considered that this minimizes D2 ingress and hence the potential for PT hydriding. Unit 4 is scheduled to shut down for retubing in May 1991. Throughout the design it has been ensured that the leak detection capability of the AGS is not impaired by O2 addition. (author). 4 refs., 6 figs

  10. MicNeSs: genotyping microsatellite loci from a collection of (NGS) reads.

    Science.gov (United States)

    Suez, Marie; Behdenna, Abdelkader; Brouillet, Sophie; Graça, Paula; Higuet, Dominique; Achaz, Guillaume

    2016-03-01

    Microsatellites are widely used in population genetics to uncover recent evolutionary events. They are typically genotyped using capillary sequencer, which capacity is usually limited to 9, at most 12 loci for each run, and which analysis is a tedious task that is performed by hand. With the rise of next-generation sequencing (NGS), a much larger number of loci and individuals are available from sequencing: for example, on a single run of a GS Junior, 28 loci from 96 individuals are sequenced with a 30X cover. We have developed an algorithm to automatically and efficiently genotype microsatellites from a collection of reads sorted by individual (e.g. specific PCR amplifications of a locus or a collection of reads that encompass a locus of interest). As the sequencing and the PCR amplification introduce artefactual insertions or deletions, the set of reads from a single microsatellite allele shows several length variants. The algorithm infers, without alignment, the true unknown allele(s) of each individual from the observed distributions of microsatellites length of all individuals. MicNeSs, a python implementation of the algorithm, can be used to genotype any microsatellite locus from any organism and has been tested on 454 pyrosequencing data of several loci from fruit flies (a model species) and red deers (a nonmodel species). Without any parallelization, it automatically genotypes 22 loci from 441 individuals in 11 hours on a standard computer. The comparison of MicNeSs inferences to the standard method shows an excellent agreement, with some differences illustrating the pros and cons of both methods.

  11. Next Generation Solvent (NGS): Development for Caustic-Side Solvent Extraction of Cesium

    Energy Technology Data Exchange (ETDEWEB)

    Moyer, Bruce A. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Birdwell, Jr, Joseph F. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Bonnesen, Peter V. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Bruffey, Stephanie H. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Delmau, Laetitia Helene [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Duncan, Nathan C. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Ensor, Dale [Tennessee Technological Univ., Cookeville, TN (United States); Hill, Talon G. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Lee, Denise L. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Rajbanshi, Arbin [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Roach, Benjamin D. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Szczygiel, Patricia L. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Sloop, Jr., Frederick V. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Stoner, Erica L. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Williams, Neil J. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

    2014-03-01

    This report summarizes the FY 2010 and 2011 accomplishments at Oak Ridge National Laboratory (ORNL) in developing the Next Generation Caustic-Side Solvent Extraction (NG-CSSX) process, referred to commonly as the Next Generation Solvent (NGS), under funding from the U.S. Department of Energy, Office of Environmental Management (DOE-EM), Office of Technology Innovation and Development. The primary product of this effort is a process solvent and preliminary flowsheet capable of meeting a target decontamination factor (DF) of 40,000 for worst-case Savannah River Site (SRS) waste with a concentration factor of 15 or higher in the 18-stage equipment configuration of the SRS Modular Caustic-Side Solvent Extraction Unit (MCU). In addition, the NG-CSSX process may be readily adapted for use in the SRS Salt Waste Processing Facility (SWPF) or in supplemental tank-waste treatment at Hanford upon appropriate solvent or flowsheet modifications. Efforts in FY 2010 focused on developing a solvent composition and process flowsheet for MCU implementation. In FY 2011 accomplishments at ORNL involved a wide array of chemical-development activities and testing up through single-stage hydraulic and mass-transfer tests in 5-cm centrifugal contactors. Under subcontract from ORNL, Argonne National Laboratory (ANL) designed a preliminary flowsheet using ORNL cesium distribution data, and Tennessee Technological University confirmed a chemical model for cesium distribution ratios (DCs) as a function of feed composition. Interlaboratory efforts were coordinated with complementary engineering tests carried out (and reported separately) by personnel at Savannah River National Laboratory (SRNL) and Savannah River Remediation (SRR) with helpful advice by Parsons Engineering and General Atomics on aspects of possible SWPF implementation.

  12. 42 CFR 419.50 - Annual review.

    Science.gov (United States)

    2010-10-01

    ... 42 Public Health 3 2010-10-01 2010-10-01 false Annual review. 419.50 Section 419.50 Public Health... review. (a) General rule. Not less often than annually, CMS reviews and updates groups, relative payment... selection of representatives of providers to review (and advise CMS concerning) the clinical integrity...

  13. 42 CFR 51.8 - Annual reports.

    Science.gov (United States)

    2010-10-01

    ... 42 Public Health 1 2010-10-01 2010-10-01 false Annual reports. 51.8 Section 51.8 Public Health... PROTECTION AND ADVOCACY FOR INDIVIDUALS WITH MENTAL ILLNESS PROGRAM Basic Requirements § 51.8 Annual reports... Date Note: At 62 FR 53564, Oct. 15, 1997, § 51.8 was added. This section contains...

  14. Darlington NGS vacuum building outage: modification to supply post-seismic makeup water to the heat transport system

    International Nuclear Information System (INIS)

    The periodic stationwide outage at Darlington NGS includes removal of the Emergency Coolant Injection System from service to allow for maintenance. As a prerequisite, the station must set up a minimum 2.5 kg/s temporary makeup water supply, to help cool the fuel should a seismic event occur. This modification cannot be commissioned, as commissioning would dilute the heavy water in the Primary Heat Transport System with light water. Therefore, flow analysis is necessary to demonstrate that the required flow rate can be achieved. This paper provides details of the design, and key aspects of the supporting analysis. (author)

  15. Best estimate and uncertainty analysis of a critical large break loss of coolant accident at Darlington NGS

    International Nuclear Information System (INIS)

    This paper briefly describes the development and application of a Best Estimate Analysis with Uncertainty (BEAU) methodology to a critical large break loss of coolant accident at Darlington NGS. Best estimate and uncertainty predictions are obtained for maximum fuel centreline temperature, maximum fuel sheath temperature, maximum fuel string relative axial expansion, and peak pressure tube strain. The results are compared against similar results obtained using the Limit of Operating Envelope (LOE) approach reported in the most recent licensing submission. The comparison shows significant improvements in predicted safety margins can be achieved. (author)

  16. Modelling of blast wave propagation through the Darlington NGS powerhouse in the event of a nearby accidental explosion

    International Nuclear Information System (INIS)

    The propagation of a blast wave inside the Darlington Nuclear Generating Station NGS) powerhouse following an accidental explosion at a nearby railway line has been studied numerically using two different computer codes. Pressurization of the building interior at various locations in the powerhouse are computed with an Ontario Hydro in-house finite-difference containment code called PATRIC, and in one benchmark case with an Institute for Aerospace Studies code based on the random-choice method, both of which solve unsteady one-dimensional flow problems. Some pertinent results of both codes are presented, and they are also shown to be in good agreement

  17. A duck RH panel and its potential for assisting NGS genome assembly

    Directory of Open Access Journals (Sweden)

    Rao Man

    2012-09-01

    Full Text Available Abstract Background Owing to the low cost of the high throughput Next Generation Sequencing (NGS technology, more and more species have been and will be sequenced. However, de novo assemblies of large eukaryotic genomes thus produced are composed of a large number of contigs and scaffolds of medium to small size, having no chromosomal assignment. Radiation hybrid (RH mapping is a powerful tool for building whole genome maps and has been used for several animal species, to help assign sequence scaffolds to chromosomes and determining their order. Results We report here a duck whole genome RH panel obtained by fusing female duck embryonic fibroblasts irradiated at a dose of 6,000 rads, with HPRT-deficient Wg3hCl2 hamster cells. The ninety best hybrids, having an average retention of 23.6% of the duck genome, were selected for the final panel. To allow the genotyping of large numbers of markers, as required for whole genome mapping, without having to cultivate the hybrid clones on a large scale, three different methods involving Whole Genome Amplification (WGA and/or scaling down PCR volumes by using the Fluidigm BioMarkTM Integrated Fluidic Circuits (IFC Dynamic ArrayTM for genotyping were tested. RH maps of APL12 and APL22 were built, allowing the detection of intrachromosomal rearrangements when compared to chicken. Finally, the panel proved useful for checking the assembly of sequence scaffolds and for mapping EST located on one of the smallest microchromosomes. Conclusion The Fluidigm BioMarkTM Integrated Fluidic Circuits (IFC Dynamic ArrayTM genotyping by quantitative PCR provides a rapid and cost-effective method for building RH linkage groups. Although the vast majority of genotyped markers exhibited a picture coherent with their associated scaffolds, a few of them were discordant, pinpointing potential assembly errors. Comparative mapping with chicken chromosomes GGA21 and GGA11 allowed the detection of the first chromosome rearrangements

  18. Revised dose limits and new respiratory tract model and their implications for annual limits of intake of radioactive materials - A review of recent ICRP publications

    International Nuclear Information System (INIS)

    Ionizing radiation may cause immediate and/or delayed biological damages to the body of the exposed person and/or his/her progeny. The exposure may be caused by an external source or may arise due to internal contamination by a radioactive material. In order to prevent such exposure, or to reduce the probability that it will occur, national authorities and international organizations that are engaged in radiation safety and protection have set limits for the exposure to ionizing radiation from either source. The sensitivity of the body to ionizing radiation usually decreases with age. For this reason and due to the limited possibilities to control the exposure of the general public, different limits have been set for for occupational exposure and for the exposure of members of the public of different age groups. The general principles of these limits and guidelines for their calculations are set by the International Commission on Radiological Protection (ICRP) and published in the Annals of the ICRP. The basic philosophy of the Commission, which includes the principles of justification, optimization and dose limits, the basic radiobiological models, and the distinction between stochastic and non-stochastic effects has been presented in its publication no. 26 . Based on this philosophy, the Commission issued between 1979 and 1988 a series of publications followed by annexes and addenda known as publication no. 30 . This series presented models describing the metabolism of radioactive materials which enter the body by inhalation and ingestion, the transfer of such materials from the respiratory tract and the gastrointestinal tract to the blood, and from there to the body organs and the excretion of the material from the body. This series presented also values for biokinetic parameters of these systems and transfer paths, and methods for calculating limits on intake which ensure that the exposure from internal contamination will not exceed the dose limits set by the

  19. Annual Energy Review 2007

    Energy Technology Data Exchange (ETDEWEB)

    Seiferlein, Katherine E. [USDOE Energy Information Administration (EIA), Washington, DC (United States)

    2008-06-01

    The Annual Energy Review (AER) is the Energy Information Administration's (EIA) primary report of annual historical energy statistics. For many series, data begin with the year 1949. Included are data on total energy production, consumption, and trade; overviews of petroleum, natural gas, coal, electricity, nuclear energy, renewable energy, international energy, as well as financial and environment indicators; and data unit conversion tables. Publication of this report is required under Public Law 95–91 (Department of Energy Organization Act), Section 205(c), and is in keeping with responsibilities given to the EIA under Section 205(a)(2), which states: “The Administrator shall be responsible for carrying out a central, comprehensive, and unified energy data and information program which will collect, evaluate, assemble, analyze, and disseminate data and information....”

  20. International energy annual 1997

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1999-04-01

    The International Energy Annual presents an overview of key international energy trends for production, consumption, imports, and exports of primary energy commodities in over 220 countries, dependencies, and areas of special sovereignty. Also included are population and gross domestic product data, as well as prices for crude oil and petroleum products in selected countries. Renewable energy reported in the International Energy Annual includes hydroelectric power and geothermal, solar, and wind electric power. Also included are biomass electric power for Brazil and the US, and biomass, geothermal, and solar energy produced in the US and not used for electricity generation. This report is published to keep the public and other interested parties fully informed of primary energy supplies on a global basis. The data presented have been largely derived from published sources. The data have been converted to units of measurement and thermal values (Appendices E and F) familiar to the American public. 93 tabs.

  1. NAGRA Annual report 2010

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2011-04-15

    This annual report presents the highlights of the activities carried out by the Swiss National Co-operative for the Disposal of Radioactive Wastes NAGRA during the year 2010. These include reviews by various commissions of the NAGRA co-operative's proposals for possible sites for nuclear waste repositories. Also, the enhancements made concerning information facilities for the general public at the co-operative's rock laboratories are mentioned. The operation of initial satellite-based precision measurement systems for movements in the earth's crust is noted. Organisational aspects and international co-operation are discussed. This annual report also looks at NAGRA's organisational structures and its commercial accounts. Appendices provide details on waste inventories and volumes and publications made in 2010. A selection of relevant internet addresses is also provided

  2. NAGRA Annual report 2010

    International Nuclear Information System (INIS)

    This annual report presents the highlights of the activities carried out by the Swiss National Co-operative for the Disposal of Radioactive Wastes NAGRA during the year 2010. These include reviews by various commissions of the NAGRA co-operative's proposals for possible sites for nuclear waste repositories. Also, the enhancements made concerning information facilities for the general public at the co-operative's rock laboratories are mentioned. The operation of initial satellite-based precision measurement systems for movements in the earth's crust is noted. Organisational aspects and international co-operation are discussed. This annual report also looks at NAGRA's organisational structures and its commercial accounts. Appendices provide details on waste inventories and volumes and publications made in 2010. A selection of relevant internet addresses is also provided

  3. Custo anual do manejo da cardiopatia isquêmica crônica no Brasil: perspectiva pública e privada Annual cost of ischemic heart disease in Brazil: public and private perspective

    Directory of Open Access Journals (Sweden)

    Rodrigo A. Ribeiro

    2005-07-01

    Full Text Available OBJETIVO: Estimar o custo anual do manejo da doença arterial coronária (DAC em valores do SUS e convênios. MÉTODOS: Estudo de coorte, incluindo pacientes ambulatoriais com DAC comprovada. Considerou-se para estimar custos diretos: consultas, exames, procedimentos, internações e medicamentos. Valores de consultas e exames foram obtidos da tabela SUS e da Lista de Procedimentos Médicos (LPM. Valores de eventos cardiovasculares foram obtidos de internações em hospital público e privado com estas classificações diagnósticas em 2002. O preço dos fármacos utilizado foi o de menor custo no mercado. RESULTADOS: Os 147 pacientes (65±12 anos, 63% homens, 69% hipertensos, 35% diabéticos e 59% com IAM prévio tiveram acompanhamento médio de 24±8 meses. O custo anual médio estimado por paciente foi de R$ 2.733,00, pelo SUS, e R$ 6.788,00, para convênios. O gasto com medicamentos ($ 1.154,00 representou 80% e 55% dos custos ambulatoriais, e 41% e 17% dos gastos totais, pelo SUS e para convênios, respectivamente. A ocorrência de evento cardiovascular teve grande impacto (R$ 4.626,00 vs. R$ 1.312,00, pelo SUS, e R$ 13.453,00 vs. R$ 1.789,00, para convênios, pOBJECTIVE: To estimate the annual cost of coronary artery disease (CAD management in Public Health Care System (SUS and HMOs values in Brazil. METHODS: Cohort study, including ambulatory patients with proven CAD. Clinic visits, exams, procedures, hospitalizations and medications were considered to estimate direct costs. Values of appointments and exams were obtained from the SUS and the Medical Procedure List (LPM 1999 reimbursement tables. Costs of cardiovascular events were obtained from admissions in public and private hospitals with similar diagnoses-related group classifications in 2002. The price of medications used was the lowest found in the market. RESULTS: The 147 patients (65±12 years old, 63% men, 69% hypertensive, 35% diabetic and 59% with previous AMI had an average

  4. Risoe annual report 1987

    International Nuclear Information System (INIS)

    An explanation of Risoe National Laboratory's function within the Danish research system is followed by brief accounts of research activities at Risoe during 1987. Energy resources, technology and policy are discussed, the annual accounts are presented, a guide to the National Laboratory and a list of its publications are given. Some of the research activities that took place in 1987 described in more detail are within the fields of chemistry and the environment, superconductivity, new aspects of powdery mildew, polymers and robotics. (AB)

  5. TIARA annual report 1999

    Energy Technology Data Exchange (ETDEWEB)

    Saidoh, Masahiro; Toraishi, Akio; Itoh, Hisayoshi [eds.] [Japan Atomic Energy Research Inst., Takasaki, Gunma (Japan). Takasaki Radiation Chemistry Research Establishment] [and others

    2000-10-01

    This annual report describes research and development activities which have been performed with the JAERI TIARA (Takasaki Ion Accelerators for Advanced Radiation Application) facilities from April 1, 1999 to March 31, 2000. Summary reports of 106 papers and brief descriptions on the status of TIARA in the period are contained. A list of publications, the type of research collaborations and organization of TIARA are also given as appendices. (author)

  6. TIARA annual report 1997

    Energy Technology Data Exchange (ETDEWEB)

    Tanaka, Ryuichi; Saido, Masahiro; Nashiyama, Isamu [eds.] [Japan Atomic Energy Research Inst., Takasaki, Gunma (Japan). Takasaki Radiation Chemistry Research Establishment] [and others

    1998-10-01

    This annual report describes research activities which have been performed with the JAERI TIARA (Takasaki Ion Accelerators for Advanced Radiation Application) facilities from April 1, 1997 to March 31, 1998. Summary reports of 90 papers and brief descriptions on the status of TIARA in the period are contained. A list of publications, the type of research collaborations and organization of TIARA are also given as appendices. (author)

  7. TIARA annual report 2003

    International Nuclear Information System (INIS)

    This annual report describes research and development activities which have been performed with the JAERI TIARA (Takasaki Ion Accelerators for Advanced Radiation Application) facilities from April 1, 2003 to March 31, 2004. Summary reports of 115 papers and brief descriptions on the status of TIARA in the period are contained. A list of publications, the type of research collaborations and organization of TIARA are also given as appendices. (author)

  8. TIARA annual report 1996

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-11-01

    This annual report describes research activities which have been performed with the JAERI TIARA (Takasaki Ion Accelerators for Advanced Radiation Application) facilities from April 1, 1996 to March 31, 1997. Summary reports of 88 papers and 4 brief descriptions on the status of TIARA in the period are contained. A list of publications, the type of research collaborations and organization of TIARA are also given as appendices. (J.P.N.)

  9. TIARA annual report 2000

    Energy Technology Data Exchange (ETDEWEB)

    Saidoh, Masahiro; Toraishi, Akio; Namba, Hideki (eds.) [Japan Atomic Energy Research Inst., Takasaki, Gunma (Japan). Takasaki Radiation Chemistry Research Establishment] [and others

    2001-11-01

    This annual report describes research and development activities which have been performed with the JAERI TIARA (Takasaki Ion Accelerators for Advanced Radiation Application) facilities from April 1, 2000 to March 31, 2001. Summary reports of 103 papers and brief descriptions on the status of TIARA in the period are contained. A list of publications, the type of research collaborations and organization of TIARA are also given as appendices. (author)

  10. Annual Report 1979

    International Nuclear Information System (INIS)

    This annual report from the Netherlands Centre for Energy Research, includes the progress made in the five main research areas: fission energy, nuclear fusion and superconductivity, combustion energy (including environmental research), current energy and non-energetic applications of nuclear fission. Studies performed by the Energy Study Centrum, a department within ECN, and the Bureau for Energy Research Projects are described. A financial report is presented and a list of publications included. (C.F.)

  11. TIARA annual report 2001

    International Nuclear Information System (INIS)

    This annual report describes research and development activities which have been performed with the JAERI TIARA (Takasaki Ion Accelerators for Advanced Radiation Application) facilities from April 1, 2001 to March 31, 2002. Summary reports of 109 papers and brief descriptions on the status of TIARA in the period are contained. A list of publications, the type of research collaborations and organization of TIARA are also given as appendices. (author)

  12. TIARA annual report 1996

    International Nuclear Information System (INIS)

    This annual report describes research activities which have been performed with the JAERI TIARA (Takasaki Ion Accelerators for Advanced Radiation Application) facilities from April 1, 1996 to March 31, 1997. Summary reports of 88 papers and 4 brief descriptions on the status of TIARA in the period are contained. A list of publications, the type of research collaborations and organization of TIARA are also given as appendices. (J.P.N.)

  13. TIARA annual report 1999

    International Nuclear Information System (INIS)

    This annual report describes research and development activities which have been performed with the JAERI TIARA (Takasaki Ion Accelerators for Advanced Radiation Application) facilities from April 1, 1999 to March 31, 2000. Summary reports of 106 papers and brief descriptions on the status of TIARA in the period are contained. A list of publications, the type of research collaborations and organization of TIARA are also given as appendices. (author)

  14. TIARA annual report 1997

    International Nuclear Information System (INIS)

    This annual report describes research activities which have been performed with the JAERI TIARA (Takasaki Ion Accelerators for Advanced Radiation Application) facilities from April 1, 1997 to March 31, 1998. Summary reports of 90 papers and brief descriptions on the status of TIARA in the period are contained. A list of publications, the type of research collaborations and organization of TIARA are also given as appendices. (author)

  15. TIARA annual report 1998

    International Nuclear Information System (INIS)

    This annual report describes research and development activities which have been performed with the JAERI TIARA (Takasaki Ion Accelerators for Advanced Radiation Application) facilities from April 1, 1998 to March 31, 1999. Summary reports of 95 papers and brief descriptions on the status of TIARA in the period are contained. A list of publications, the type of research collaborations and organization of TIARA are also given as appendices. (author)

  16. TIARA annual report 2002

    International Nuclear Information System (INIS)

    This annual report describes research and development activities which have been performed with the JAERI TIARA (Takasaki Ion Accelerators for Advanced Radiation Application) facilities from April 1, 2002 to March 31, 2003. Summary reports of 113 papers and brief descriptions on the status of TIARA in the period are contained. A list of publications, the type of research collaborations and organization of TIARA are also given as appendices. (author)

  17. TIARA annual report 1998

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1999-10-01

    This annual report describes research and development activities which have been performed with the JAERI TIARA (Takasaki Ion Accelerators for Advanced Radiation Application) facilities from April 1, 1998 to March 31, 1999. Summary reports of 95 papers and brief descriptions on the status of TIARA in the period are contained. A list of publications, the type of research collaborations and organization of TIARA are also given as appendices. (author)

  18. TIARA annual report 2001

    Energy Technology Data Exchange (ETDEWEB)

    Saidoh, Masahiro; Ohara, Yoshihiro; Namba, Hideki (eds.) [Japan Atomic Energy Research Inst., Takasaki, Gunma (Japan). Takasaki Radiation Chemistry Research Establishment] [and others

    2002-11-01

    This annual report describes research and development activities which have been performed with the JAERI TIARA (Takasaki Ion Accelerators for Advanced Radiation Application) facilities from April 1, 2001 to March 31, 2002. Summary reports of 109 papers and brief descriptions on the status of TIARA in the period are contained. A list of publications, the type of research collaborations and organization of TIARA are also given as appendices. (author)

  19. TIARA annual report 2000

    International Nuclear Information System (INIS)

    This annual report describes research and development activities which have been performed with the JAERI TIARA (Takasaki Ion Accelerators for Advanced Radiation Application) facilities from April 1, 2000 to March 31, 2001. Summary reports of 103 papers and brief descriptions on the status of TIARA in the period are contained. A list of publications, the type of research collaborations and organization of TIARA are also given as appendices. (author)

  20. Report to Congress in response to Public Law 99-240: 1990 Annual report on low-level radioactive waste management progress

    International Nuclear Information System (INIS)

    This report summarizes the progress during 1990 of states and compact regions in establishing new low-level radioactive waste disposal facilities. It also provides summary information on the volume of low-level radioactive waste received for disposal in 1990 by commercially operated low-level radioactive waste disposal facilities. This report is in response to section 7 (b) of the Low-Level Radioactive Waste Policy Act, as amended by Public Law 99-240

  1. NGS-QCbox and Raspberry for Parallel, Automated and Rapid Quality Control Analysis of Large-Scale Next Generation Sequencing (Illumina) Data.

    Science.gov (United States)

    Katta, Mohan A V S K; Khan, Aamir W; Doddamani, Dadakhalandar; Thudi, Mahendar; Varshney, Rajeev K

    2015-01-01

    Rapid popularity and adaptation of next generation sequencing (NGS) approaches have generated huge volumes of data. High throughput platforms like Illumina HiSeq produce terabytes of raw data that requires quick processing. Quality control of the data is an important component prior to the downstream analyses. To address these issues, we have developed a quality control pipeline, NGS-QCbox that scales up to process hundreds or thousands of samples. Raspberry is an in-house tool, developed in C language utilizing HTSlib (v1.2.1) (http://htslib.org), for computing read/base level statistics. It can be used as stand-alone application and can process both compressed and uncompressed FASTQ format files. NGS-QCbox integrates Raspberry with other open-source tools for alignment (Bowtie2), SNP calling (SAMtools) and other utilities (bedtools) towards analyzing raw NGS data at higher efficiency and in high-throughput manner. The pipeline implements batch processing of jobs using Bpipe (https://github.com/ssadedin/bpipe) in parallel and internally, a fine grained task parallelization utilizing OpenMP. It reports read and base statistics along with genome coverage and variants in a user friendly format. The pipeline developed presents a simple menu driven interface and can be used in either quick or complete mode. In addition, the pipeline in quick mode outperforms in speed against other similar existing QC pipeline/tools. The NGS-QCbox pipeline, Raspberry tool and associated scripts are made available at the URL https://github.com/CEG-ICRISAT/NGS-QCbox and https://github.com/CEG-ICRISAT/Raspberry for rapid quality control analysis of large-scale next generation sequencing (Illumina) data.

  2. NGS-QCbox and Raspberry for Parallel, Automated and Rapid Quality Control Analysis of Large-Scale Next Generation Sequencing (Illumina Data.

    Directory of Open Access Journals (Sweden)

    Mohan A V S K Katta

    Full Text Available Rapid popularity and adaptation of next generation sequencing (NGS approaches have generated huge volumes of data. High throughput platforms like Illumina HiSeq produce terabytes of raw data that requires quick processing. Quality control of the data is an important component prior to the downstream analyses. To address these issues, we have developed a quality control pipeline, NGS-QCbox that scales up to process hundreds or thousands of samples. Raspberry is an in-house tool, developed in C language utilizing HTSlib (v1.2.1 (http://htslib.org, for computing read/base level statistics. It can be used as stand-alone application and can process both compressed and uncompressed FASTQ format files. NGS-QCbox integrates Raspberry with other open-source tools for alignment (Bowtie2, SNP calling (SAMtools and other utilities (bedtools towards analyzing raw NGS data at higher efficiency and in high-throughput manner. The pipeline implements batch processing of jobs using Bpipe (https://github.com/ssadedin/bpipe in parallel and internally, a fine grained task parallelization utilizing OpenMP. It reports read and base statistics along with genome coverage and variants in a user friendly format. The pipeline developed presents a simple menu driven interface and can be used in either quick or complete mode. In addition, the pipeline in quick mode outperforms in speed against other similar existing QC pipeline/tools. The NGS-QCbox pipeline, Raspberry tool and associated scripts are made available at the URL https://github.com/CEG-ICRISAT/NGS-QCbox and https://github.com/CEG-ICRISAT/Raspberry for rapid quality control analysis of large-scale next generation sequencing (Illumina data.

  3. NGS-QCbox and Raspberry for Parallel, Automated and Rapid Quality Control Analysis of Large-Scale Next Generation Sequencing (Illumina) Data.

    Science.gov (United States)

    Katta, Mohan A V S K; Khan, Aamir W; Doddamani, Dadakhalandar; Thudi, Mahendar; Varshney, Rajeev K

    2015-01-01

    Rapid popularity and adaptation of next generation sequencing (NGS) approaches have generated huge volumes of data. High throughput platforms like Illumina HiSeq produce terabytes of raw data that requires quick processing. Quality control of the data is an important component prior to the downstream analyses. To address these issues, we have developed a quality control pipeline, NGS-QCbox that scales up to process hundreds or thousands of samples. Raspberry is an in-house tool, developed in C language utilizing HTSlib (v1.2.1) (http://htslib.org), for computing read/base level statistics. It can be used as stand-alone application and can process both compressed and uncompressed FASTQ format files. NGS-QCbox integrates Raspberry with other open-source tools for alignment (Bowtie2), SNP calling (SAMtools) and other utilities (bedtools) towards analyzing raw NGS data at higher efficiency and in high-throughput manner. The pipeline implements batch processing of jobs using Bpipe (https://github.com/ssadedin/bpipe) in parallel and internally, a fine grained task parallelization utilizing OpenMP. It reports read and base statistics along with genome coverage and variants in a user friendly format. The pipeline developed presents a simple menu driven interface and can be used in either quick or complete mode. In addition, the pipeline in quick mode outperforms in speed against other similar existing QC pipeline/tools. The NGS-QCbox pipeline, Raspberry tool and associated scripts are made available at the URL https://github.com/CEG-ICRISAT/NGS-QCbox and https://github.com/CEG-ICRISAT/Raspberry for rapid quality control analysis of large-scale next generation sequencing (Illumina) data. PMID:26460497

  4. International energy annual, 1986

    International Nuclear Information System (INIS)

    The International Energy Annual presents current data and trends for production, consumption, stocks, imports, and exports for primary energy commodities in more than 190 countries, dependencies, and areas of special sovereignty. Also included are prices on crude petroleum and petroleum products in selected countries. This report is published to keep the public and other interested parties fully informed with respect to primary energy supplies on a global basis. The data presented have been largely derived from published sources and from United States Embassy personnel in foreign posts. The data have been converted to units of measurement and thermal values familiar to the American public

  5. Using GC-FID to Quantify the Removal of 4-sec-Butylphenol from NGS Solvent by NaOH

    Energy Technology Data Exchange (ETDEWEB)

    Sloop, Jr., Frederick V. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Moyer, Bruce A. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

    2014-12-01

    A caustic wash of the solvent used in the Next-Generation Caustic-Side Solvent Extraction (NG-CSSX) process was found to remove the modifier breakdown product 4-sec-butylphenol (SBP) with varying efficiency depending on the aqueous NaOH concentration. Recent efforts at ORNL have aimed at characterizing the flowsheet chemistry and reducing the technical uncertainties of the NG-CSSX process. One technical uncertainty has been the efficacy of caustic washing of the solvent for the removal of lipophilic anions, in particular, the efficient removal of SBP, an important degradation product of the solvent modifier, Cs-7SB. In order to make this determination, it was necessary to develop a sensitive and reliable analytical technique for the detection and quantitation of SBP. This report recounts the development of a GC-FID-based (Gas Chromatography Flame Ionization Detection) technique for analyzing SBP and the utilization of the technique to subsequently confirm the ability of the caustic wash to efficiently remove SBP from the Next Generation Solvent (NGS) used in NG-CSSX. In particular, the developed technique was used to monitor the amount of SBP removed from a simple solvent and the full NGS by contact with sodium hydroxide wash solutions over a range of concentrations. The results show that caustic washing removes SBP with effectively the same efficiency as it did in the original Caustic-Side Solvent Extraction (CSSX) process.

  6. Petroleum marketing annual 1993

    International Nuclear Information System (INIS)

    The Petroleum Marketing Annual (PMA) contains statistical data on a variety of crude oils and refined petroleum products. The publication provides statistics on crude oil costs and refined petroleum products sales for use by industry, government, private sector analysts, educational institutions, and consumers. Data on crude oil include the domestic first purchase price, the free-on-board (f.o.b.) and landed cost of imported crude oil, and the refiners acquisition cost of crude oil. Sales data for motor gasoline, distillates, residuals, aviation fuels, kerosene, and propane are presented. For this publication, all estimates have been recalculated since their earlier publication in the Petroleum Marketing Monthly (PMM). These calculations made use of additional data and corrections that were received after the PMM publication dates

  7. Petroleum marketing annual, 1991

    International Nuclear Information System (INIS)

    The Petroleum Marketing Annual contains statistical data on a variety of crude oils and refined petroleum products. The publication provides statistics on crude oil costs and refined petroleum products sales for use by industry, government, private sector analysts, educational institutions, and consumers. Data on crude oil include the domestic first purchase price, the free-on-board and landed cost of imported crude oil, and the refiners' acquisition cost of crude oil. Sales data for motor gasoline, distillates, residuals, aviation fuels, kerosene, and propane are presented. For this publication, all estimates have been recalculated since their earlier publication in the Petroleum Marketing Monthly (PMM). These calculations made use of additional data and corrections that were received after the PMM publication dates

  8. Climatological Data Publication

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Major US airport weather stations monthly and annual publication containing station daily maximum and minimum temperatures, precipitation amounts, and monthly...

  9. Federal energy conservation programs pursuant to section 381 of the Energy Policy and Conservation Act (Public Law 94-163). Annual report to Congress

    Energy Technology Data Exchange (ETDEWEB)

    1979-02-21

    This report provides an overview of the activities and achievements of the executive branch of the Federal Government in implementing the energy conservation requirements and provisions of section 381 of the Energy Policy and Conservation Act (EPCA) of 1975 (Public Law 94-163). The report describes Federal actions to develop procurement policies that promote energy conservation and efficiency, develop a Federal 10-Year Buildings Energy Conservation Plan, develop responsible public education and information programs, encourage energy conservation and energy efficiency, and promote vanpooling and carpooling arrangements. About half of the Nation's energy is used in our homes and automobiles. Another 48 percent is used by State and local governments, business and insutry, in providing needed goods and services. The Federal Government is the Nation's largest energy user, accouting for 2.2 percent of the total national energy used in 1977. This energy is used by nearly 6 million people in more than 400 thousand buildings and in the operation of more than 600 thousand vehicles. While energy conservation and energy efficiency measures alone cannot solve our immediate problems, they are an essential part of our transition to an era of scarce and expensive energy supplies.

  10. Annual Energy Review 2011

    Energy Technology Data Exchange (ETDEWEB)

    Fichman, Barbara T. [USDOE Energy Information Administration (EIA), Washington, DC (United States)

    2012-09-01

    The Annual Energy Review (AER) is the U.S. Energy Information Administration's (EIA) primary report of annual historical energy statistics. For many series, data begin with the year 1949. Included are statistics on total energy production, consumption, trade, and energy prices; overviews of petroleum, natural gas, coal, electricity, nuclear energy, and renewable energy; financial and environment indicators; and data unit conversions. Publication of this report is required under Public Law 95–91 (Department of Energy Organization Act), Section 205(c), and is in keeping with responsibilities given to the EIA under Section 205(a)(2), which states: “The Administrator shall be responsible for carrying out a central, comprehensive, and unified energy data and information program which will collect, evaluate, assemble, analyze, and disseminate data and information....” The AER is intended for use by Members of Congress, Federal and State agencies, energy analysts, and the general public. EIA welcomes suggestions from readers regarding the content of the AER and other EIA publications.

  11. Electric power annual 1992

    Energy Technology Data Exchange (ETDEWEB)

    1994-01-06

    The Electric Power Annual presents a summary of electric utility statistics at national, regional and State levels. The objective of the publication is to provide industry decisionmakers, government policymakers, analysts and the general public with historical data that may be used in understanding US electricity markets. The Electric Power Annual is prepared by the Survey Management Division; Office of Coal, Nuclear, Electric and Alternate Fuels; Energy Information Administration (EIA); US Department of Energy. ``The US Electric Power Industry at a Glance`` section presents a profile of the electric power industry ownership and performance, and a review of key statistics for the year. Subsequent sections present data on generating capability, including proposed capability additions; net generation; fossil-fuel statistics; retail sales; revenue; financial statistics; environmental statistics; electric power transactions; demand-side management; and nonutility power producers. In addition, the appendices provide supplemental data on major disturbances and unusual occurrences in US electricity power systems. Each section contains related text and tables and refers the reader to the appropriate publication that contains more detailed data on the subject matter. Monetary values in this publication are expressed in nominal terms.

  12. Annual Energy Review 2009

    Energy Technology Data Exchange (ETDEWEB)

    Fichman, Barbara T. [USDOE Energy Information Administration (EIA), Washington, DC (United States)

    2010-08-01

    The Annual Energy Review (AER) is the U.S. Energy Information Administration's (EIA) primary report of annual historical energy statistics. For many series, data begin with the year 1949. Included are statistics on total energy production, consumption, trade, and energy prices; overviews of petroleum, natural gas, coal, electricity, nuclear energy, renewable energy, and international energy; financial and environment indicators; and data unit conversions. Publication of this report is required under Public Law 95–91 (Department of Energy Organization Act), Section 205(c), and is in keeping with responsibilities given to the EIA under Section 205(a)(2), which states: “The Administrator shall be responsible for carrying out a central, comprehensive, and unified energy data and information program which will collect, evaluate, assemble, analyze, and disseminate data and information....” The AER is intended for use by Members of Congress, Federal and State agencies, energy analysts, and the general public. EIA welcomes suggestions from readers regarding the content of the AER and other EIA publications.

  13. Annual Energy Review 2005

    Energy Technology Data Exchange (ETDEWEB)

    Seiferlein, Katherine E. [USDOE Energy Information Administration (EIA), Washington, DC (United States)

    2006-07-01

    The Annual Energy Review (AER) is the Energy Information Administration's (EIA) primary report of annual historical energy statistics. For many series, data begin with the year 1949. Included are data on total energy production, consumption, and trade; overviews of petroleum, natural gas, coal, electricity, nuclear energy, renewable energy, international energy, as well as financial and environment indicators; and data unit conversion tables. Publication of this report is required under Public Law 95–91 (Department of Energy Organization Act), Section 205(c), and is in keeping with responsibilities given to the EIA under Section 205(a)(2), which states: “The Administrator shall be responsible for carrying out a central, comprehensive, and unified energy data and information program which will collect, evaluate, assemble, analyze, and disseminate data and information....” The AER is intended for use by Members of Congress, Federal and State agencies, energy analysts, and the general public. EIA welcomes suggestions from readers regarding data series in the AER and in other EIA publications.

  14. Annual Energy Review 2004

    Energy Technology Data Exchange (ETDEWEB)

    Seiferlein, Katherine E. [USDOE Energy Information Administration (EIA), Washington, DC (United States)

    2005-08-01

    The Annual Energy Review (AER) is the Energy Information Administration's (EIA) primary report of annual historical energy statistics. For many series, data begin with the year 1949. Included are data on total energy production, consumption, and trade; overviews of petroleum, natural gas, coal, electricity, nuclear energy, renewable energy, international energy, as well as financial and environment indicators; and data unit conversion tables. Publication of this report is required under Public Law 95–91 (Department of Energy Organization Act), Section 205(c), and is in keeping with responsibilities given to the EIA under Section 205(a)(2), which states: “The Administrator shall be responsible for carrying out a central, comprehensive, and unified energy data and information program which will collect, evaluate, assemble, analyze, and disseminate data and information....” The AER is intended for use by Members of Congress, Federal and State agencies energy analysts, and the general public. EIA welcomes suggestions from readers regarding data series in the AER and in other EIA publications.

  15. Annual Energy Review 2006

    Energy Technology Data Exchange (ETDEWEB)

    Seiferlein, Katherine E. [USDOE Energy Information Administration (EIA), Washington, DC (United States)

    2007-06-01

    The Annual Energy Review (AER) is the Energy Information Administration's (EIA) primary report of annual historical energy statistics. For many series, data begin with the year 1949. Included are data on total energy production, consumption, and trade; overviews of petroleum, natural gas, coal, electricity, nuclear energy, renewable energy, international energy, as well as financial and environment indicators; and data unit conversion tables. Publication of this report is required under Public Law 95–91 (Department of Energy Organization Act), Section 205(c), and is in keeping with responsibilities given to the EIA under Section 205(a)(2), which states: “The Administrator shall be responsible for carrying out a central, comprehensive, and unified energy data and information program which will collect, evaluate, assemble, analyze, and disseminate data and information....” The AER is intended for use by Members of Congress, Federal and State agencies, energy analysts, and the general public. EIA welcomes suggestions from readers regarding data series in the AER and in other EIA publications.

  16. The failure of the pressure tube in fuel channel NO6 of Bruce NGS-A unit 2 in March 1986

    International Nuclear Information System (INIS)

    The events leading up to the failure of Bruce NGS-A Unit 2 pressure tube NO6 are described. Based on the subsequent examination of the tube, reactor operating history and pressure tube manufacturing information, the failure sequence is deduced. Remedial actions which have been adopted for avoiding similar occurrences are listed

  17. ICO amplicon NGS data analysis: a Web tool for variant detection in common high-risk hereditary cancer genes analyzed by amplicon GS Junior next-generation sequencing.

    Science.gov (United States)

    Lopez-Doriga, Adriana; Feliubadaló, Lídia; Menéndez, Mireia; Lopez-Doriga, Sergio; Morón-Duran, Francisco D; del Valle, Jesús; Tornero, Eva; Montes, Eva; Cuesta, Raquel; Campos, Olga; Gómez, Carolina; Pineda, Marta; González, Sara; Moreno, Victor; Capellá, Gabriel; Lázaro, Conxi

    2014-03-01

    Next-generation sequencing (NGS) has revolutionized genomic research and is set to have a major impact on genetic diagnostics thanks to the advent of benchtop sequencers and flexible kits for targeted libraries. Among the main hurdles in NGS are the difficulty of performing bioinformatic analysis of the huge volume of data generated and the high number of false positive calls that could be obtained, depending on the NGS technology and the analysis pipeline. Here, we present the development of a free and user-friendly Web data analysis tool that detects and filters sequence variants, provides coverage information, and allows the user to customize some basic parameters. The tool has been developed to provide accurate genetic analysis of targeted sequencing of common high-risk hereditary cancer genes using amplicon libraries run in a GS Junior System. The Web resource is linked to our own mutation database, to assist in the clinical classification of identified variants. We believe that this tool will greatly facilitate the use of the NGS approach in routine laboratories.

  18. 2005 Annual report

    International Nuclear Information System (INIS)

    This annual report presents information on the activities, on the scope of the radiation protection and nuclear safety, of the Nuclear Regulatory Authority (ARN) of the Argentina during 2005. The main topics are the water measurements associated with the expert's report of Ezeiza, the emergency systems, the environmental control, the institutional relations and the non-proliferation, the training and the public information, the safeguards and the physical protection, the quality assurance in the ARN, the environmental monitoring, and the human and economic resources. Also, this publication has annexes with the following content: the regulatory framework; regulatory documents; inspections to medical, industrial and training installations; international expert's report on the application of the radiological protection international standards of the public at the Ezeiza Atomic Center zone; ethical code

  19. LIEKKI 2 - Annual Review 1997. Reports of the review group. Publication list 1993-1996; LIEKKI 2 - Vuosikirja 1997. Seurantaryhmaeraportit. LIEKKI 2 - tutkimusohjelman julkaisuluettelo 1993-1996

    Energy Technology Data Exchange (ETDEWEB)

    Hupa, M.; Matinlinna, J. [eds.

    1997-10-01

    The LIEKKI 2 program is planned to cover the research work to be performed in the period beginning in 1993 and ending in 1998. LIEKKI 2 is largely a continuation of the combustion and gasification research earlier included in the national research programmes LIEKKI and JALO. The research within the scope of the LIEKKI 2 research programme is aimed at supporting the development of energy conversion techniques relating to combustion and gasification in Finland. Research serving the development of new, more efficient and environmentally sound techniques will receive special attention, but research developing conventional combustion techniques is also to be included in the programme. Another important objective of the programme is to maintain and develop the competence of different research groups in this field of technology on a long term basis. The main research areas are: Modelling of the furnace processes; The chemistry of gaseous emission components; Ash, aerosols and the behaviour of particles; New combustion and gasification technologies; Black liquor and Conventional combustion technologies and waste incineration. The part of the book contains the reports of the review group chairmen for 1996 and a list of LIEKKI 2 publications 1993-1996. (orig.)

  20. Flow-induced vibration and acoustic behaviour of CANFLEX-LVRF bundles in a Bruce B NGS fuel channel

    International Nuclear Information System (INIS)

    Frequency/temperature sweep tests were performed in a high-temperature/high-pressure test channel to determine the acoustic and flow-induced vibration characteristics of the CANFLEX-LVRF bundle. The vibratory response of CANFLEX-LVRF bundles was compared with that of 37-element fuel bundles under Bruce B NGS fuel channel normal operating conditions. The tests were performed with a 12-bundle string of CANFLEX-LVRF bundles as well as a mixed string for the transition core. The tests showed that the LVRF bundles performed as required without failure or gross geometry changes. The mixed fuel strings behaved in a manner similar to that of a string of CANFLEX-LVRF bundles. (author)

  1. Review of the analysis of a failure to shutdown following a large LOCA in a Pickering NGS A reactor unit

    International Nuclear Information System (INIS)

    A review has been undertaken of the study performed by Ontario Hydro, with the assistance of Argonne National Laboratory, on the nature and consequences of a failure to shut down (LOSD) following a large LOCA in a Pickering A NGS reactor unit. Ontario Hydro analyzed the complete accident sequence, from the initiating LOCA and LOSD to containment behaviour and off-site radiological consequences. Argonne, working independently, analyzed only fuel behaviour, fuel channel response and failure, and moderator response. Both studies were in very close agreement. Off-site radiological consequences of the accident were not found to be worse than those of any severe dual-failure accident analyzed during the licensing process and thus to be within the regulatory limits from such accidents. Recommendations for further work are given

  2. Characterization of fuel failures resulting from the 1988 November overpower transient in Pickering NGS-A Unit 1

    International Nuclear Information System (INIS)

    During 1988 November a recovery from a reactor trip in Pickering NGS-A Unit 1 resulted in an unexpected number of power ramp-related fuel failures. Subsequent examinations at Chalk River Nuclear Laboratories revealed the defect cause to be stress corrosion cracking of the Zircaloy 4 sheathing. Higher than expected sheath residual strains were observed. These are believed to be related to the physical properties of the UO2 fuel (i.e. density, clearances and pellet geometry). In addition, higher than predicted fission gas releases and anomalous UO2 pellet centre grain growth behaviour were observed. Further work has been initiated to characterize better the Pickering fuel behaviour to assist in improved fuel modeling capability (e.g. ELESIM). A revised correlation has been developed to predict more accurately the onset and extent of fuel failures. (Author) 5 refs., 5 tabs., 7 figs

  3. Grid平台上的NGS编译技术%The Compiler Technology of NGS on the Grid

    Institute of Scientific and Technical Information of China (English)

    丛杨; 王雷; 朱凯佳; 刘又诚

    2003-01-01

    随着网络技术的发展,在异构平台上使用共同的计算和信息资源将很快成为可能.Grid(网格)就是这样一种提供资源共享的新兴平台,而在其之上的下一代软件程序(NGS)则对编译器提出了新的挑战[1].未来Grid平台上的编译系统将是能够进行动态编译和优化,根据实时系统以及网络的性能不断进行自我调整的软件模型,同时它还能为具有自适应性的应用程序提供编译支持.

  4. Implementering av vertikala vindkraftverk på lyktstolpar : En hållberhetsstudie längs Essingeleden

    OpenAIRE

    Tapia Chiriboga, Camilo; Mars Bodell, Joel

    2015-01-01

    I takt med att de icke-förnyelsebara energikällorna minskar och att man ser ett ökat världsligt energibehov behövs nya, effektiva förnyelsebara energikällor såsom den vertikalaxlade vindkraften. Denna rapport syftar till att studera olika modeller av vertikala vindkraftverk för att ta reda på vilken modell som är bäst lämpad att på ett hållbart sätt driva ett system av lyktstolpar längs Essingeleden. Studien undersöker modellernas hållbarhet ur ett tekniskt, miljömässigt, socialt och ekonomis...

  5. Detailed finite element analysis of Darlington NGS feeder pipes with locally thinned regions below pressure minimum thickness

    International Nuclear Information System (INIS)

    Feeder Pipes in CANDU nuclear stations are experiencing wall thinning due to flow accelerated corrosion (FAC) resulting in locally thinned regions in addition to general thinning. In Darlington NGS these locally thinned regions can be below pressure based minimum thickness (tmin), required as per ASME Code Section III NB-3600 Equation (1). A methodology is presented to qualify the locally thinned regions under NB-3200 (NB-3213 and NB-3221) for internal pressure loading only. Detailed finite element models are used for internal pressure analysis using ANSYS v11.0. All other loadings such as deadweight, thermal and seismic loadings are qualified under NB-3600 using a general purpose piping stress analysis software. The piping stress analysis is based on average thickness equal to tmin along with maximum values of ASME Code stress indices (Table NB-3681(a)-1). The requirement for the use of this methodology is that the average thickness of each cross-section with the locally thinned region shall be at least tmin. The finite element analysis models are thinned to 0.75 tmin (in increments of 0.05 tmin) all-around the circumference in the straight section region allowing for flexible inspection requirements. Two different thicknesses of 1.10 tmin and 1.30 tmin are assigned to the bends. Thickness vs the allowable axial extent curves were developed for different types of feeder pipes in service. Feeders differ in pipe size, straight section length, bend angle and orientation. The stress analysis results show that all Darlington NGS outlet feeder pipes are fit for service with locally thinned regions up to 75% of the pressure based minimum thickness. This paper demonstrates the effectiveness of finite element analysis in extending the useful life of degraded piping components. (author)

  6. NGS metabarcoding proves successful for quantitative assessment of symbiont abundance: the case of feather mites on birds.

    Science.gov (United States)

    Diaz-Real, J; Serrano, D; Piriz, A; Jovani, R

    2015-10-01

    Understanding the ecological function of species and the structure of communities is crucial in the study of ecological interactions among species. For this purpose, not only the occurrence of particular species but also their abundance in ecological communities is required. However, abundance quantification of species through morphological characters is often difficult or time/money consuming when dealing with elusive or small taxa. Here we tested the use of next-generation sequencing (NGS) for abundance estimation of two species of feather mites (Proctophyllodes stylifer and Pteronyssoides parinus) under five proportions (16:1, 16:4, 16:16, 16:64, and 16:256 mites) against a mock community composed by Proctophyllodes clavatus and Proctophyllodes sylviae. In all mixtures, we retrieved sequence reads from all species. We found a strong linear relationship between 454 reads and the real proportion of individuals in the mixture for both focal species. The slope for Pr. stylifer was close to one (0.904), and the intercept close to zero (-0.007), thus showing an almost perfect correspondence between real and estimated proportions. The slope for Pt. parinus was 0.351 and the intercept 0.307, showing that while the estimated proportion increased linearly relative to real proportions of individuals in the samples, proportions were overestimated at low real proportions and underestimated at larger ones. Additionally, pyrosequencing replicates from each DNA extraction were highly repeatable (R = 0.920 and 0.972, respectively), showing that the quantification method is highly consistent given a DNA extract. Our study suggests that NGS is a promising tool for abundance estimation of feather mites' communities in birds.

  7. Points to consider in the clinical use of NGS panels for mitochondrial disease: an analysis of gene inclusion and consent forms.

    Science.gov (United States)

    Platt, Julia; Cox, Rachel; Enns, Gregory M

    2014-08-01

    Mitochondrial next generation sequencing (NGS) panels offer single-step analysis of the numerous nuclear genes involved in the structure, function, and maintenance of mitochondria. However, the complexities of mitochondrial biology and genetics raise points for consideration in clinical use of these tests. To understand the current status of mitochondrial genetic testing, we assessed the gene offerings and consent forms of mitochondrial NGS panels available from seven US-based clinical laboratories. The NGS panels varied markedly in number of genes (101-1204 genes), and the proportion of genes causing "classic" mitochondrial diseases and their phenocopies ranged widely between labs (18 %-94 % of panel contents). All panels included genes not associated with classic mitochondrial diseases (6 %-28 % of panel contents), including genes causing adult-onset neurodegenerative disorders, cancer predisposition, and other genetic syndromes or inborn errors of metabolism. Five of the panels included genes that are not listed in OMIM to be associated with a disease phenotype (5 %-49 % of panel contents). None of the consent documents reviewed had options for patient preference regarding receipt of incidental findings. These findings raise points of discussion applicable to mitochondrial diagnostics, but also to the larger arenas of exome and genome sequencing, including the need to consider the boundaries between clinical and research testing, the necessity of appropriate informed consent, and the responsibilities of clinical laboratories and clinicians. Based on these findings, we recommend careful evaluation by laboratories of the genes offered on NGS panels, clear communication of the predicted phenotypes, and revised consent forms to allow patients to make choices about receiving incidental findings. We hope that our analysis and recommendations will help to maximize the considerable clinical utility of NGS panels for the diagnosis of mitochondrial disease.

  8. Annual report on the activities of National Reference Centers established on the basis of regional public health authorities of the Slovak Republic and the Office of Public Health of the Slovak Republic in 2008

    International Nuclear Information System (INIS)

    A brief account of activities carried out by the National Reference Centers (NRC) established on the basis of regional public health authorities of the Slovak Republic and the Office of Public Health of the Slovak Republic in 2008 is presented. These activities are reported under the headings: (1) NRC for influenza; (2) NRC for arbovirus and hemorrhagic fever; (3) NRC for poliomyelitis; (4) NRC for meningococci; (5) NRC to monitor the resistance of microorganisms to antibiotics; (6) NRC for evaluation of late effects of chemical substances, methods of genetic toxicology; (7) NRC for hydrobiology; (8) NRC for ecotoxicology; (9) NRC for non-ionizing radiation; (10) NRC for hydrothermal microclimate; (11) NRC for exposure tests xenobiotics; (12) NRC for morbilli, rubella and parotitis; (13) NRC for environmental microbiology; (14) NRC on pesticide residues; (15) NRC for salmonella; (16) NRC for microbial autovaccines; (17) NRC health issues for urban air pollution; (18) NRC for noise and vibration; (19) Information Center for bacteriological (biological) and toxin weapons; (20) NRC for impact assessment of ambient air and air of enclosed spaces non-productive nature of population health; (21) NRC for toxoplasmosis; (22) NRC for exposure assessment and health risk; (23) NRC for pertussis and parapertussis; (24) NRC for syphilis; (25) NRC for hygienic drinking water problems; (26) NRC for listeriosis; (27) NRC for intestinal parasitosis; (28) NRC for diphtheria; (29) NRC for vibrionaceae; (30) NRC for environmental mycology; (31) NRC issues for fibrous dusts; (32) NRC for thermotolerant amoebae; (33) NRC issues for coal mines; (34) NRC for packaging materials; (35) NRC for haemophilus infections; (36) NRC for Legionella.

  9. Annual report on the activities of National Reference Centers established on the basis of regional public health authorities of the Slovak Republic and the Office of Public Health of the Slovak Republic in 2009

    International Nuclear Information System (INIS)

    A brief account of activities carried out by the National Reference Centers (NRC) established on the basis of regional public health authorities of the Slovak Republic and the Office of Public Health of the Slovak Republic in 2009 is presented. These activities are reported under the headings: (1) NRC for influenza; (2) NRC for arbovirus and hemorrhagic fever; (3) NRC for poliomyelitis; (4) NRC for meningococci; (5) NRC to monitor the resistance of microorganisms to antibiotics; (6) NRC for evaluation of late effects of chemical substances, methods of genetic toxicology; (7) NRC for hydrobiology; (8) NRC for ecotoxicology; (9) NRC for non-ionizing radiation; (10) NRC for hydrothermal microclimate; (11) NRC for exposure tests xenobiotics; (12) NRC for morbilli, rubella and parotitis; (13) NRC for environmental microbiology; (14) NRC on pesticide residues; (15) NRC for salmonella; (16) NRC health issues for urban air pollution; (17) NRC for microbial autovaccines; (18) NRC for noise and vibration; (19) Information Center for bacteriological (biological) and toxin weapons; (20) NRC for impact assessment of ambient air and air of enclosed spaces non-productive nature of population health; (21) NRC for toxoplasmosis; (22) NRC for exposure assessment and health risk; (23) NRC for pertussis and parapertussis; (24) NRC for syphilis; (25) NRC for hygienic drinking water problems; (26) NRC for listeriosis; (27) NRC for intestinal parasitosis; (28) NRC for diphtheria; (29) NRC for vibrionaceae; (30) NRC for environmental mycology; (31) NRC issues for fibrous dusts; (32) NRC for thermotolerant amoebae; (33) NRC issues for coal mines; (34) NRC for articles of daily use and packing materials; (35) NRC for haemophilus infections; (36) NRC for Legionella in the environment.

  10. Annual report of the National Reference Centres established on the basis of regional public health authorities of the Slovak Republic and the Office of Public Health of the Slovak Republic. Year 2011

    International Nuclear Information System (INIS)

    A brief account of activities carried out by the National Reference Centres (NRC) established on the basis of regional public health authorities of the Slovak Republic and the Office of Public Health of the Slovak Republic. (UVZ SR) in 2011 is presented. These activities are reported under the headings: (1) NRC for flu; (2) NRC for arbovirus and hemorrhagic fever; (3) NRC for poliomyelitis; (4) NRC for meningococci; (5) NRC to monitor the resistance of microorganisms to antibiotics; (6) NRC for morbilli, rubella and parotitis; (7) NRC for salmonellosis; (8) NRC for evaluation of late effects of chemicals by methods of genetic toxicology; (9) NRC for hydrobiology; (10) NRC for ecotoxicology; (11) NRC for non-ionizing radiation; (12) NRC for hydrothermal microclimate; (13) NRC tests for exposure of xenobiotics; (14) NRC for environmental microbiology; (15) NRC for Legionella in the environment; (16) NRC on pesticide residues; (17) The Information Centre for bacteriological (biological) and toxin weapons; (18) NRC for impact assessment of ambient air pollution and an enclosed non-productive nature of the health of the population; (19) NRC for toxoplasmosis; (20) NRC Assessment of personal exposure and health risk; (21) NRC for pertussis and parapertussis; (22) NRC for pneumococcal infection; (23) NRC for syphilis; (24) NRC for hygienic drinking water problems; (25) NRC for listeriosis; (26) NRC for intestinal parasites; (27) NRC for diphtheria; (28) NRC for coagulase-positive staphylococci and their toxins; (29) NRC for Vibrionaceae; (30) NRC for environmental mycology; (31) NRC for noise and vibration; (32) NRC for commonly used items and packaging materials; (33) NRC for thermotolerant amoebae; (34) NRC issues for coal mines; (35) NRC for work physiology and ergonomics; (37) NRC for health issues fibrous dusts; (38) NRC for haemophilus infection.

  11. Annual report of the National Reference Centres established on the basis of regional public health authorities of the Slovak Republic and the Office of Public Health of the Slovak Republic. Year 2010

    International Nuclear Information System (INIS)

    A brief account of activities carried out by the National Reference Centres (NRC) established on the basis of regional public health authorities of the Slovak Republic and the Office of Public Health of the Slovak Republic. (UVZ SR) in 2010 is presented. These activities are reported under the headings: (1) NRC for flu; (2) NRC for arbovirus and hemorrhagic fever; (3) NRC for poliomyelitis; (4) NRC for meningococci; (5) NRC to monitor the resistance of microorganisms to antibiotics; (6) NRC for morbilli, rubella and parotitis; (7) NRC for salmonellosis; (8) NRC for evaluation of late effects of chemicals by methods of genetic toxicology; (9) NRC for hydrobiology; (10) NRC for ecotoxicology; (11) NRC for non-ionizing radiation; (12) NRC for hydrothermal microclimate; (13) NRC tests for exposure of xenobiotics; (14) NRC for environmental microbiology; (15) NRC for Legionella in the environment; (16) NRC on pesticide residues; (17) The Information Centre for bacteriological (biological) and toxin weapons; (18) NRC for impact assessment of ambient air pollution and an enclosed non-productive nature of the health of the population; (19) NRC for toxoplasmosis; (20) NRC Assessment of personal exposure and health risk; (21) NRC for pertussis and parapertussis; (22) NRC for syphilis; (23) NRC for hygienic drinking water problems; (24) NRC for listeriosis; (25) NRC for intestinal parasites; (26) NRC for diphtheria; (27) NRC for coagulase-positive staphylococci and their toxins; (28) NRC for Vibrionaceae; (29) NRC for environmental mycology; (30) NRC for noise and vibration; (31) NRC for health issues fibrous dusts; (32) NRC for thermotolerant amoebae; (33) NRC issues for coal mines; (34) NRC for materials in contact with food; (35) NRC for haemophilus infection; (36) Dedicated site for viral hepatitis.

  12. Annual report of the National Reference Centres established on the basis of regional public health authorities of the Slovak Republic and the Office of Public Health of the Slovak Republic. Year 2012

    International Nuclear Information System (INIS)

    A brief account of activities carried out by the National Reference Centres (NRC) established on the basis of regional public health authorities of the Slovak Republic and the Office of Public Health of the Slovak Republic. (UVZ SR) in 2012 is presented. These activities are reported under the headings: (1) NRC for flu; (2) NRC for arbovirus and hemorrhagic fever; (3) NRC for poliomyelitis; (4) NRC for meningococci; (5) NRC to monitor the resistance of microorganisms to antibiotics; (6) NRC for morbilli, rubella and parotitis; (7) NRC for salmonellosis; (8) NRC for evaluation of late effects of chemicals by methods of genetic toxicology; (9) NRC for hydrobiology; (10) NRC for ecotoxicology; (11) NRC for non-ionizing radiation; (12) NRC for hydrothermal microclimate; (13) NRC tests for exposure of xenobiotics; (14) NRC for environmental microbiology; (15) NRC for Legionella in the environment; (16) NRC on pesticide residues; (17) NRC for impact assessment of ambient air pollution and an enclosed non-productive nature of the health of the population; (18) NRC for toxoplasmosis; (19) NRC Assessment of personal exposure and health risk; (20) NRC for pertussis and parapertussis; (21) NRC for pneumococcal disease; (22) NRC for syphilis; (23) NRC for hygienic drinking water problems; (24) NRC for listeriosis; (25) The National Information Centre for bacteriological (biological) and toxin weapons; (26) NRC for intestinal parasites; (27) NRC for diphtheria; (28) NRC for coagulase-positive staphylococci and their toxins; (29) NRC for Vibrionaceae; (30) NRC for environmental mycology; (31) NRC for noise and vibration; (32) NRC for articles of daily use and packaging materials; (33) NRC for thermotolerant amoebae; (34) NRC issues for coal mines; (35) NRC to work physiology and ergonomics; (36) NRC for health issues fibrous dusts; (37) NRC for haemophilus infection; (38) NRC for cosmetic products.

  13. Coal industry annual 1997

    International Nuclear Information System (INIS)

    Coal Industry Annual 1997 provides comprehensive information about US coal production, number of mines, prices, productivity, employment, productive capacity, and recoverable reserves. US Coal production for 1997 and previous years is based on the annual survey EIA-7A, Coal Production Report. This report presents data on coal consumption, coal distribution, coal stocks, coal prices, and coal quality for Congress, Federal and State agencies, the coal industry, and the general public. Appendix A contains a compilation of coal statistics for the major coal-producing States. This report includes a national total coal consumption for nonutility power producers that are not in the manufacturing, agriculture, mining, construction, or commercial sectors. 14 figs., 145 tabs

  14. Coal industry annual 1997

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-12-01

    Coal Industry Annual 1997 provides comprehensive information about US coal production, number of mines, prices, productivity, employment, productive capacity, and recoverable reserves. US Coal production for 1997 and previous years is based on the annual survey EIA-7A, Coal Production Report. This report presents data on coal consumption, coal distribution, coal stocks, coal prices, and coal quality for Congress, Federal and State agencies, the coal industry, and the general public. Appendix A contains a compilation of coal statistics for the major coal-producing States. This report includes a national total coal consumption for nonutility power producers that are not in the manufacturing, agriculture, mining, construction, or commercial sectors. 14 figs., 145 tabs.

  15. Pickering NGS A reactor building 1 dome refurbishment long-term monitoring of coating

    International Nuclear Information System (INIS)

    'Full text:' To reduce air leakage through the dome of Pickering NGS A Reactor Building 1, in August 1993 a portion of the exterior concrete surface was coated with a single component elastomeric polyurethane material. An internal positive pressure test of the building, conducted between November 5 and 7, 1993, found that the air leakage rates were significantly lower in this test than leakage rates which had been measured during a pressure test conducted in 1992. This reduction in leakage was attributed to the successful performance of the coating. The need for a high-performance, elastomeric surface coating was identified for reduction of air leakage levels through the dome of Reactor Building l of Ontario Power Generation's (formerly Ontario Hydro's) Pickering 'A' Nuclear Generating Station near Toronto. A number of candidate coatings were extensively tested to assess the performance characteristics and identify a material that could withstand the elements and perform effectively for around 20 years. Under normal operating conditions, a licensing limit of 2.7% of contained mass/hour is set for permissible containment leakage whilst the operational working target is less than 1%. The facility's engineers determined that any leakages were pressure-dependent, so in an effort to remain well within their working target, they sought a system that would bridge and seal any hairline cracks in the concrete dome and thereby prevent the passage of gas or vapour through the substrate. On the basis of scheduling and cost, they concluded that a high performance coating was most appropriate for the project, and hired Kinectrics (formerly Ontario Hydro Technologies (OHT)) to select, test, assess and arrange for the application to the RB 1 Dome. In all, nearly 70 separate manufacturers were approached by Kinectrics with a view to obtaining recommendations for treatment. The respective performance data of the respondents' products were compared with a set of specific design

  16. Annual report 1979/1980

    International Nuclear Information System (INIS)

    The annual report concentrates result of investigations in the field of the production of radioisotopes, the synthesis of radioactive compounds and their characterization. Main topics are the production of 99Mo and the application of radioactive compounds in nuclear medicine especially as radiopharmaceuticals. A list of publications and lectures is enclosed. (author)

  17. Strategic petroleum reserve annual report

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-02-15

    Section 165 of the Energy Policy and Conservation Act (Public Law 94- 163), as amended, requires the Secretary of Energy to submit annual reports to the President and the Congress on activities of the Strategic Petroleum Reserve (SPR). This report describes activities for the year ending December 31, 1995.

  18. Annual report 1985 Chemistry Department

    International Nuclear Information System (INIS)

    This annual report describes the activities carried out in 1985 by the Chemistry Department in the following fields: Chemistry, Inorganic Chemistry, Physicochemistry (Interphases, Surfaces), General Chemical Analysis, Active Materials Analysis, X Ray Fluorescence Analysis, Mass Spectroscopy (Isotopic Analysis, Instrumentation) and Optical Spectroscopy. A list of publications is enclosed. (M.E.L.)

  19. JAERI tandem annual report, 1982

    International Nuclear Information System (INIS)

    This annual report describes research activities which have been performed with JAERI tandem accelerator from September 1, 1981 to March 31, 1983. Summary reports of 38 papers, publications, personnel and a list of co-operative researches with universities are contained. (author)

  20. JAERI Tandem annual report 1983

    International Nuclear Information System (INIS)

    This annual report describes research activities which have been performed with JAERI tandem accelerator from April 1, 1983 to March 31, 1984. Summary reports of 32 papers, publications, personnel and a list of co-operative reserches with universities are contained. (author)

  1. Bundesforschungsanstalt fuer Ernaehrung. Annual report

    International Nuclear Information System (INIS)

    The 1994 annual report of the Bundesforschungsanstalt fuer Ernaehrung describes the tasks, structure and staff and fields of research of this institution and its scientific cooperation with other institutions. The report is supplemented by an alphabetic list off all publications. The BFE specializes in research on nutritional, food and ecotrophological sciences. (vhe)

  2. Annual report to Congress

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1992-03-01

    This is the eighth annual report submitted by the Office of Civilian Radioactive Waste Management (OCRWM) to Congress. It covers activities and expenditures during Fiscal Year 1991, which ended September 30, 1991. Chapter 1 of this report describes OCRWM`s mission and objectives. Chapters 2 through 8 cover the following topics: earning public trust and confidence; geological disposal; monitored retrieval storage; transportation; systems integration and regulatory compliance; international programs; and program management. Financial statements for the Nuclear Waste Fund are presented in Chapter 9.

  3. Annual report 2002

    International Nuclear Information System (INIS)

    This annual report presents the CEA (Atomic Energy Research Center) activities and research programs during the year 2002. the first part is devoted to the scientific development in the defense, nuclear energy, technology, fundamental research and valuation domains. The second and third parts present respectively the development conditions with the environment safety and the means of development with the human resources, the information technology, the training and the public relations. The fourth part situates the Cea enterprise in the economy and the fifth part the Cea development in the Europe and the world. The last part is the financial accounting. (A.L.B.)

  4. Annual Report 1981

    International Nuclear Information System (INIS)

    This Annual Report covers the activities at the Kernfysisch Versneller Instituut (KVI) during the year 1981. It contains 74 short reports grouped under the headings: inelastic scattering studies, transfer and charge-exchange reactions, breakup reactions and fission studies, heavy-ion reactions, gamma, electron and X-ray spectroscopy, hyperfine interactions and nuclear orientation, weak interactions, IBA related studies, other theoretical studies, cyclotron and beam lines, data acquisition and data analysis, instrumentation and electronics and medical physics. Details are also presented of the personnel, scientific publications, internal reports, and attended and internal conferences and seminars. (C.F.)

  5. IKF annual report 1983

    International Nuclear Information System (INIS)

    This annual report contains extended abstracts about the scientific work performed at the named institute descriptions of the operation of the Van-de-Graaf accelerator facilities of this institute and the work of the technical establishments, as well as a list of publications. The scientific work concerns nuclear structure and nuclear reactions, high energy heavy ion physics, atomic physics with fast ions, nuclear solid state physics, solid-state track detectors, applications of nuclear methods in solid state physics, ion source developments, apparative developments and data processing, as well as interdisciplinary collaborations. See hints under the relevant topics. (HSI)

  6. Annual Report 2007

    International Nuclear Information System (INIS)

    The Annual Report of the Institute of Nuclear Chemistry and Technology (Warsaw, Poland) describes achievements of the Institute in 2007 obtained in seven fields: (1) radiation chemistry and physics, radiation technologies; (2) radiochemistry, stable isotopes, nuclear analytical methods, general chemistry; (3) radiobiology; (4) nuclear technologies and methods: process engineering; (5) nuclear technologies and methods: material engineering, structural studies, diagnostics; (6) nuclear technologies and methods: nucleonic control systems and accelerators. In total - 76 detailed papers prepared by the Institute workers and collaborating scientists are presented. General information on the Institute status, personnel activity, international cooperation and publications are also given

  7. Annual Report 1993

    International Nuclear Information System (INIS)

    An overview of electricity generation by thermal and nuclear power plants as well as by non-conventional energy sources is presented, and the condition of the power transmission system is dealt with. The information presented concerns the electricity and heat generation business, company economy and finance, business activity development, environmental aspects, human resources and social policy, and public relations. Basic data of the annual statement as of 31 December 1993 are given. The assets of the company were CZK 97,231,604, the profit was CZK 7,278,946. (J.B.)

  8. Annual report 1980

    International Nuclear Information System (INIS)

    This annual report contains extended abstracts about the research done at the named institute. These abstracts concern the development of accelerators and ion sources, the construction of the magnetic spectrograph and radiation detectors, the investigation of solids and microstructures by nuclear methods, the development of electronic circuits, the advances in data processing, the study of heavy ion reactions, nuclear structure, and reaction mechanisms, the research on atomic physics and the interaction of charged particles with matter, the studies in medium and high energy physics, the theoretical studies of nuclear structure, and the research in cosmochemistry. Furthermore a list of publications is added. (orig./HSI)

  9. Annual report 1979

    International Nuclear Information System (INIS)

    This annual report contains extended abstracts about the research done at the named institute. These abstracts concern the development of accelerators and ion sources, the construction of the magnetic spectrograph and radiation detectors, the investigation of solids and microstructures by nuclear methods, the development of electronic circuits, the advances in data processing, the study of heavy ion reactions, nuclear structure, and reaction mechanisms, the research on atomic physics and the interaction of charged particles with matter, the studies in medium and high energy physics, the theoretical studies of nuclear structure, and the research in cosmochemistry. Furthermore a list of publications is added. (orig./HSI)

  10. Annual report 1981

    International Nuclear Information System (INIS)

    This annual report contains extended abstracts about the research done at the named institute. These abstracts concern the development of accelerators and ion sources, the construction of the magnetic spectrograph and radiation detectors, the investigation of solids and microstructures by nuclear methods, the development of electronic circuits, the advances in data processing, the study of heavy ion reactions, nuclear structure, and reaction mechanisms, the research on atomic physics and the interaction of charged particles with matter, the studies of in medium and high energy physics, the theoretical studies of nuclear structure and the research in cosmophysics. Furthermore a list of publications is added. (orig./HSI)

  11. Annual Report 2006

    International Nuclear Information System (INIS)

    The Annual Report of the Institute of Nuclear Chemistry and Technology (Warsaw, Poland) describes achievements of the Institute in 2006 obtained in seven fields: (1) radiation chemistry and technology, (2) radiochemistry and coordination chemistry, (3) radiobiology, (4) application of nuclear methods in material and process engineering, (5) design of instruments based on nuclear techniques, (6) trace analysis and radioanalytical techniques, (7) environmental research. In total - 83 detailed papers prepared by the Institute workers and collaborating scientists are presented. General information on the Institute status, personnel activity, international cooperation and publications are presented

  12. Annual Report 2008

    International Nuclear Information System (INIS)

    The Annual Report of the Institute of Nuclear Chemistry and Technology (Warsaw, Poland) describes achievements of the Institute in 2008 obtained in six fields: (1) radiation chemistry and physics, radiation technologies, (2) radiochemistry, stable isotopes, nuclear analytical methods, general chemistry, (3) radiobiology, (4) nuclear technologies and methods: process engineering, (5) nuclear technologies and methods: material engineering, structural studies, diagnostics, (6) nucleonic control systems and accelerators. In total - 76 detailed papers prepared by the Institute workers and collaborating scientists are presented. General information on the Institute status, personnel activity, international cooperation and publications are also given

  13. 1985. Annual progress report

    International Nuclear Information System (INIS)

    This annual progress report of the CEA Protection and Nuclear Safety Institut outlines a description of the progress made in each sections of the Institut Research activities of the different departments include: reactor safety analysis, fuel cycle facilities analysis; and associated safety research programs (criticality, sites, transport ...), radioecology and environmental radioprotection techniques; data acquisition on radioactive waste storage sites; radiation effects on man, studies on radioprotection techniques; nuclear material security including security of facilities, security of nuclear material transport, and monitoring of nuclear material management; nuclear facility decommissioning; and finally the public information

  14. Petroleum marketing annual, 1990

    International Nuclear Information System (INIS)

    The Petroleum Marketing Annual contains statistical data on a variety of crude oils and refined petroleum products. The publication provides statistics on crude oil costs and refined petroleum products sales for use by industry, government, private sector analysts, educational institutions, and consumers. Data on crude oil include the domestic first purchase price, the free-on-board and landed cost of imported crude oil, and the refiners' acquisition cost of crude oil. Sales data for motor gasoline, distillates, residuals, aviation fuels, kerosene, and propane are presented. (VC)

  15. GKSS annual report 1987

    International Nuclear Information System (INIS)

    GKSS-Forschungszentrum Geesthacht GmbH, one of the national research centers, carries out R and D work on conservation of resources and environment, improvement of working conditions and on increase of economic competitiveness. The activities fall into the fields energy research and technology, basic technologies, marine research and techniques, health-environment-biotechnology. The annual report contains selected research work in summaries, the report on R and D work in reactor safety, materials research, environmental and climate research as well as environmental, and underwater techniques, describes the research institutes, cooperation with external partners, the organization, the budget, personnel, publications, including patent applications, and lectures. (HK)

  16. Annual Energy Review 2002

    Energy Technology Data Exchange (ETDEWEB)

    Seiferlein, Katherine E. [USDOE Energy Information Administration (EIA), Washington, DC (United States)

    2003-10-01

    The Annual Energy Review (AER) presents the Energy Information Administration’s historical energy statistics. For many series, statistics are given for every year from 1949 through 2002. The statistics, expressed in either physical units or British thermal units, cover all major energy activities, including consumption, production, trade, stocks, and prices, for all major energy commodities, including fossil fuels, electricity, and renewable energy sources. Publication of this report is required under Public Law 95–91 (Department of Energy Organization Act), Section 205(c), and is in keeping with responsibilities given to the Energy Information Administration (EIA) under Section 205(a)(2), which states: “The Administrator shall be responsible for carrying out a central, comprehensive, and unified energy data and information program which will collect, evaluate, assemble, analyze, and disseminate data and information....” The AER is intended for use by Members of Congress, Federal and State agencies, energy analysts, and the general public. EIA welcomes suggestions from readers regarding data series in the AER and in other EIA publications. Related Publication: Readers of the AER may also be interested in EIA’s Monthly Energy Review, which presents monthly updates of many of the data in the AER. Contact our National Energy Information Center for more information.

  17. Uranium industry annual 1994

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-07-05

    The Uranium Industry Annual 1994 (UIA 1994) provides current statistical data on the US uranium industry`s activities relating to uranium raw materials and uranium marketing during that survey year. The UIA 1994 is prepared for use by the Congress, Federal and State agencies, the uranium and nuclear electric utility industries, and the public. It contains data for the 10-year period 1985 through 1994 as collected on the Form EIA-858, ``Uranium Industry Annual Survey.`` Data collected on the ``Uranium Industry Annual Survey`` (UIAS) provide a comprehensive statistical characterization of the industry`s activities for the survey year and also include some information about industry`s plans and commitments for the near-term future. Where aggregate data are presented in the UIA 1994, care has been taken to protect the confidentiality of company-specific information while still conveying accurate and complete statistical data. A feature article, ``Comparison of Uranium Mill Tailings Reclamation in the United States and Canada,`` is included in the UIA 1994. Data on uranium raw materials activities including exploration activities and expenditures, EIA-estimated resources and reserves, mine production of uranium, production of uranium concentrate, and industry employment are presented in Chapter 1. Data on uranium marketing activities, including purchases of uranium and enrichment services, and uranium inventories, enrichment feed deliveries (actual and projected), and unfilled market requirements are shown in Chapter 2.

  18. Uranium industry annual 1994

    International Nuclear Information System (INIS)

    The Uranium Industry Annual 1994 (UIA 1994) provides current statistical data on the US uranium industry's activities relating to uranium raw materials and uranium marketing during that survey year. The UIA 1994 is prepared for use by the Congress, Federal and State agencies, the uranium and nuclear electric utility industries, and the public. It contains data for the 10-year period 1985 through 1994 as collected on the Form EIA-858, ''Uranium Industry Annual Survey.'' Data collected on the ''Uranium Industry Annual Survey'' (UIAS) provide a comprehensive statistical characterization of the industry's activities for the survey year and also include some information about industry's plans and commitments for the near-term future. Where aggregate data are presented in the UIA 1994, care has been taken to protect the confidentiality of company-specific information while still conveying accurate and complete statistical data. A feature article, ''Comparison of Uranium Mill Tailings Reclamation in the United States and Canada,'' is included in the UIA 1994. Data on uranium raw materials activities including exploration activities and expenditures, EIA-estimated resources and reserves, mine production of uranium, production of uranium concentrate, and industry employment are presented in Chapter 1. Data on uranium marketing activities, including purchases of uranium and enrichment services, and uranium inventories, enrichment feed deliveries (actual and projected), and unfilled market requirements are shown in Chapter 2

  19. 2006 Annual report

    International Nuclear Information System (INIS)

    This annual report presents information on the activities of the Nuclear Regulatory Authority (ARN) of Argentina during 2006. The main topic are: the quality assurance of the ARN; the institutional communications; the licensing and inspection of nuclear power plants and critical facilities; the emergency systems; the safeguards and the physical protection; the environmental control; the institutional relations; the training and the public information. Also, this publication have annexes with the following content: the regulatory framework; regulatory documents; inspections to medical, industrial and training installations; measurement and evaluation of the drinking water of Ezeiza; international expert's report on the application of the international standards of radiological protection at the Ezeiza Atomic Center zone; ethical code

  20. 2004 Annual report

    International Nuclear Information System (INIS)

    This annual report presents information of the main activities on the scope of radiation protection and nuclear safety of the Nuclear Regulatory Authority (ARN) of the Argentina during 2004. The main topics are the regulatory framework, the national systems to supervise nuclear installations, the emergency systems, the environmental control, the laboratories of the ARN, the institutional relations and the non-proliferation, the training and the public information, the radiological and the nuclear safety inspections, the safeguards and the physical protection, the quality assurance in the ARN, the environmental monitoring, and the human and economic resources. Also, this publication has four annexes with the following content: regulatory documents; inspections to medical, industrial and training installations; ethical code; measurements and evaluations of the drinking water in Ezeiza (Argentina)

  1. Annual report 2009

    International Nuclear Information System (INIS)

    This annual report issued by the Swiss National Co-operative for the Disposal of Radioactive Waste reviews the co-operative's activities in the year 2009 and presents an overview of developments in legislation, planning procedures and funding plans. The selection of sites for the disposal of radioactive wastes in Switzerland is discussed, as is regional participation in the site selection procedures. Various technical questions are briefly addressed and work being carried out in the rock laboratories in the Swiss Alps and Jura mountains is discussed. International co-operation is reviewed and public relations issues are discussed. International collaboration in the area of radioactive waste disposal is discussed. Finally, organisational structures are described and the financial details for the year 2009 are presented. The report is completed with an appendix containing waste inventories, predicted waste volumes and listings of publications, addresses and a short glossary

  2. Nagra annual report 2008

    Energy Technology Data Exchange (ETDEWEB)

    Ammann, M. (ed.)

    2009-07-01

    This annual report issued by the Swiss National Co-operative for the Disposal of Radioactive Waste, Nagra, reviews the co-operative's activities in the year 2008 and presents an overview of developments in energy policy, planning procedures and funding plans. Energy policy and the selection of sites for the disposal of radioactive wastes in Switzerland are discussed. Various technical questions are briefly addressed and work being carried out in the rock laboratories in the Swiss Alps and Jura mountains is discussed. International co-operation is reviewed and public relations issues are discussed. Finally, organisational structures are described and Nagra's financial details for the year 2008 are presented and discussed. The report is completed with an appendix containing the co-operative's organigram, waste inventories and listings of publications, addresses and a short glossary.

  3. Nagra annual report 2007

    Energy Technology Data Exchange (ETDEWEB)

    Ammann, M. (ed.)

    2008-07-01

    This annual report issued by the Swiss National Cooperative for the Disposal of Radioactive Waste reviews the co-operative's activities in the year 2007 and presents an overview of developments in energy policy, planning procedures and funding plans. The selection of sites for the disposal of radioactive wastes in Switzerland is discussed. Various technical questions are briefly addressed and work being carried out in the rock laboratories in the Swiss Alps and Jura mountains is discussed. International co-operation is reviewed and public relations issues are discussed. Finally, organisational structures are described and the financial details for the year 2007 are presented. The report is completed with an appendix containing the co-operative's organigram, waste inventories and listings of publications, addresses and a short glossary.

  4. Nagra annual report 2007

    International Nuclear Information System (INIS)

    This annual report issued by the Swiss National Cooperative for the Disposal of Radioactive Waste reviews the co-operative's activities in the year 2007 and presents an overview of developments in energy policy, planning procedures and funding plans. The selection of sites for the disposal of radioactive wastes in Switzerland is discussed. Various technical questions are briefly addressed and work being carried out in the rock laboratories in the Swiss Alps and Jura mountains is discussed. International co-operation is reviewed and public relations issues are discussed. Finally, organisational structures are described and the financial details for the year 2007 are presented. The report is completed with an appendix containing the co-operative's organigram, waste inventories and listings of publications, addresses and a short glossary

  5. Coal industry annual 1993

    Energy Technology Data Exchange (ETDEWEB)

    1994-12-06

    Coal Industry Annual 1993 replaces the publication Coal Production (DOE/FIA-0125). This report presents additional tables and expanded versions of tables previously presented in Coal Production, including production, number of mines, Productivity, employment, productive capacity, and recoverable reserves. This report also presents data on coal consumption, coal distribution, coal stocks, coal prices, coal quality, and emissions for a wide audience including the Congress, Federal and State agencies, the coal industry, and the general public. In addition, Appendix A contains a compilation of coal statistics for the major coal-producing States. This report does not include coal consumption data for nonutility Power Producers who are not in the manufacturing, agriculture, mining, construction, or commercial sectors. This consumption is estimated to be 5 million short tons in 1993.

  6. BESSY annual report 1991

    International Nuclear Information System (INIS)

    The annual report 1991 is drawn up as in the previous years, with general information on the organisational structure of the institution and its general purposes being given ahead of the research-specific reports. The achievements and developments in the fields of machines and experiments are reported, including contributions from the Litography laboratory of the Fraunhofer Institute for Microstructural Studies and Techniques, and from the Radiometry Laboratory of the Physikalisch-Technische Bundesanstalt, followed by reports on basic research work. The machine developments are reported in varions contributions, and a list of publications shows all available and known scientific publications resulting from research work done at the BESSY installations by the various users. Diploma, doctoral and habilitation theses are listed separately. (orig.)

  7. Introduction of the Python script STRinNGS for analysis of STR regions in FASTQ or BAM files and expansion of the Danish STR sequence database to 11 STRs

    DEFF Research Database (Denmark)

    Friis, Susanne L; Buchard, Anders; Rockenbauer, Eszter;

    2016-01-01

    This work introduces the in-house developed Python application STRinNGS for analysis of STR sequence elements in BAM or FASTQ files. STRinNGS identifies sequence reads with STR loci by their flanking sequences, it analyses the STR sequence and the flanking regions, and generates a report...... with the assigned SNP-STR alleles. The main output file from STRinNGS contains all sequences with read counts above 1% of the total number of reads per locus. STR sequences are automatically named according to the nomenclature used previously and according to the repeat unit definitions in STRBase (http......01, TPOX, vWA), and Amelogenin was analysed with STRinNGS. Sequencing uncovered five common SNPs near four STRs and revealed 20 new alleles in the 207 Danes. Three short homopolymers in the D8S1179 flanking regions caused frequent sequencing errors. In 29 of 3726 allele calls (0.8%), sequences...

  8. Annual Energy Review 1993

    Energy Technology Data Exchange (ETDEWEB)

    1994-07-14

    This twelfth edition of the Annual Energy Review (AER) presents the Energy Information Administration`s historical energy statistics. For most series, statistics are given for every year from 1949 through 1993. Because coverage spans four and a half decades, the statistics in this report are well-suited to long-term trend analyses. The AER is comprehensive. It covers all major energy activities, including consumption, production, trade, stocks, and prices, for all major energy commodities, including fossil fuels and electricity. The AER also presents Energy Information Administration (EIA) statistics on some renewable energy sources. EIA estimates that its consumption series include about half of the renewable energy used in the United States. For a more complete discussion of EIA`s renewables data, see p. xix, ``Introducing Expanded Coverage of Renewable Energy Data Into the Historical Consumption Series.`` Copies of the 1993 edition of the Annual Energy Review may be obtained by using the order form in the back of this publication. Most of the data in the 1993 edition also are available on personal computer diskette. For more information about the diskettes, see the back of this publication. In addition, the data are available as part of the National Economic, Social, and Environmental Data Bank on a CD-ROM. For more information about the data bank, contact the US Department of Commerce Economics and Statistics Administration, on 202-482-1986.

  9. Uranium industry annual 1995

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-05-01

    The Uranium Industry Annual 1995 (UIA 1995) provides current statistical data on the U.S. uranium industry`s activities relating to uranium raw materials and uranium marketing. The UIA 1995 is prepared for use by the Congress, Federal and State agencies, the uranium and nuclear electric utility industries, and the public. It contains data for the period 1986 through 2005 as collected on the Form EIA-858, ``Uranium Industry Annual Survey``. Data collected on the ``Uranium Industry Annual Survey`` provide a comprehensive statistical characterization of the industry`s plans and commitments for the near-term future. Where aggregate data are presented in the UIA 1995, care has been taken to protect the confidentiality of company-specific information while still conveying accurate and complete statistical data. Data on uranium raw materials activities for 1986 through 1995 including exploration activities and expenditures, EIA-estimated reserves, mine production of uranium, production of uranium concentrate, and industry employment are presented in Chapter 1. Data on uranium marketing activities for 1994 through 2005, including purchases of uranium and enrichment services, enrichment feed deliveries, uranium fuel assemblies, filled and unfilled market requirements, uranium imports and exports, and uranium inventories are shown in Chapter 2. The methodology used in the 1995 survey, including data edit and analysis, is described in Appendix A. The methodologies for estimation of resources and reserves are described in Appendix B. A list of respondents to the ``Uranium Industry Annual Survey`` is provided in Appendix C. For the reader`s convenience, metric versions of selected tables from Chapters 1 and 2 are presented in Appendix D along with the standard conversion factors used. A glossary of technical terms is at the end of the report. 14 figs., 56 tabs.

  10. Installation of an irradiated fuel bundle discharge counter at Bruce NGS-B 3 000 MW(e) CANDU power station

    International Nuclear Information System (INIS)

    Design, manufacture and installation of an irradiated fuel bundle discharge counter for the multi-unit CANDU Bruce NGS-B Generating Station involved contributions from the International Atomic Energy Agency (Agency), designers (AECL), contractors, manufacturers, utility and the regulatory agency. The installation at Bruce NGS-B was the first made by the Agency as a retrofit to a multi-unit CANDU reactor approaching its fist critical operation, where the whole project was the responsibility of the Agency and where the original design of the reactor had not had provision for the Agency equipment. The scheduling and integration of the installation into the normal activities involved in starting up a 3 000 MW(e) multi-unit generating station were successfully achieved. The Agency has demonstrated the capability and performance of the fuel discharge counter

  11. IMGT/StatClonotype for Pairwise Evaluation and Visualization of NGS IG and TR IMGT Clonotype (AA) Diversity or Expression from IMGT/HighV-QUEST.

    Science.gov (United States)

    Aouinti, Safa; Giudicelli, Véronique; Duroux, Patrice; Malouche, Dhafer; Kossida, Sofia; Lefranc, Marie-Paule

    2016-01-01

    There is a huge need for standardized analysis and statistical procedures in order to compare the complex immune repertoires of antigen receptors immunoglobulins (IG) and T cell receptors (TR) obtained by next generation sequencing (NGS). NGS technologies generate millions of nucleotide sequences and have led to the development of new tools. The IMGT/HighV-QUEST, available since 2010, is the first global web portal for the analysis of IG and TR high throughput sequences. IMGT/HighV-QUEST provides standardized outputs for the characterization of the "IMGT clonotype (AA)" (AA for amino acids) and their comparison in up to one million sequences. Standardized statistical procedures for "IMGT clonotype (AA)" diversity or expression comparisons have recently been described, however, no tool was yet available. IMGT/StatClonotype, a new IMGT(®) tool, evaluates and visualizes statistical significance of pairwise comparisons of IMGT clonotype (AA) diversity or expression, per V (variable), D (diversity), and J (joining) gene of a given IG or TR group, from NGS IMGT/HighV-QUEST statistical output. IMGT/StatClonotype tool is incorporated in the R package "IMGTStatClonotype," with a user-friendly interface. IMGT/StatClonotype is downloadable at IMGT(®) for users to evaluate pairwise comparison of IG and TR NGS statistical output from IMGT/HighV-QUEST and to visualize, on their web browser, the statistical significance of IMGT clonotype (AA) diversity or expression, per gene, the comparative analysis of CDR-IMGT and the V-D-J associations, in immunoprofiles from normal or pathological immune responses.

  12. From Sanger to NGS: Detecting MHC (Major Histocompatibility Complex) Class II and OR (Olfactory Receptors) Genetic Variability in Italian Wolves (Canis Lupus) and relative Canids

    OpenAIRE

    Lapalombella, Silvana

    2016-01-01

    In this PhD thesis I will describe different aspects of conservation genetics and genomics of two wild Canidae species, the wolf (Canis lupus) and the golden jackal (Canis aureus), through the study of two of the most variable gene families: the Major Histocompatibility Complex genes (MHC), and Olfactory Receptors genes (OR). In order to perform these studies both Sanger and next generation sequencing (NGS) DNA techniques have been used. The background of the thesis is described in the “Gener...

  13. IMGT/StatClonotype for Pairwise Evaluation and Visualization of NGS IG and TR IMGT Clonotype (AA) Diversity or Expression from IMGT/HighV-QUEST

    Science.gov (United States)

    Aouinti, Safa; Giudicelli, Véronique; Duroux, Patrice; Malouche, Dhafer; Kossida, Sofia; Lefranc, Marie-Paule

    2016-01-01

    There is a huge need for standardized analysis and statistical procedures in order to compare the complex immune repertoires of antigen receptors immunoglobulins (IG) and T cell receptors (TR) obtained by next generation sequencing (NGS). NGS technologies generate millions of nucleotide sequences and have led to the development of new tools. The IMGT/HighV-QUEST, available since 2010, is the first global web portal for the analysis of IG and TR high throughput sequences. IMGT/HighV-QUEST provides standardized outputs for the characterization of the “IMGT clonotype (AA)” (AA for amino acids) and their comparison in up to one million sequences. Standardized statistical procedures for “IMGT clonotype (AA)” diversity or expression comparisons have recently been described, however, no tool was yet available. IMGT/StatClonotype, a new IMGT® tool, evaluates and visualizes statistical significance of pairwise comparisons of IMGT clonotype (AA) diversity or expression, per V (variable), D (diversity), and J (joining) gene of a given IG or TR group, from NGS IMGT/HighV-QUEST statistical output. IMGT/StatClonotype tool is incorporated in the R package “IMGTStatClonotype,” with a user-friendly interface. IMGT/StatClonotype is downloadable at IMGT®1 for users to evaluate pairwise comparison of IG and TR NGS statistical output from IMGT/HighV-QUEST and to visualize, on their web browser, the statistical significance of IMGT clonotype (AA) diversity or expression, per gene, the comparative analysis of CDR-IMGT and the V–D–J associations, in immunoprofiles from normal or pathological immune responses. PMID:27667992

  14. IMGT/StatClonotype for Pairwise Evaluation and Visualization of NGS IG and TR IMGT Clonotype (AA) Diversity or Expression from IMGT/HighV-QUEST.

    Science.gov (United States)

    Aouinti, Safa; Giudicelli, Véronique; Duroux, Patrice; Malouche, Dhafer; Kossida, Sofia; Lefranc, Marie-Paule

    2016-01-01

    There is a huge need for standardized analysis and statistical procedures in order to compare the complex immune repertoires of antigen receptors immunoglobulins (IG) and T cell receptors (TR) obtained by next generation sequencing (NGS). NGS technologies generate millions of nucleotide sequences and have led to the development of new tools. The IMGT/HighV-QUEST, available since 2010, is the first global web portal for the analysis of IG and TR high throughput sequences. IMGT/HighV-QUEST provides standardized outputs for the characterization of the "IMGT clonotype (AA)" (AA for amino acids) and their comparison in up to one million sequences. Standardized statistical procedures for "IMGT clonotype (AA)" diversity or expression comparisons have recently been described, however, no tool was yet available. IMGT/StatClonotype, a new IMGT(®) tool, evaluates and visualizes statistical significance of pairwise comparisons of IMGT clonotype (AA) diversity or expression, per V (variable), D (diversity), and J (joining) gene of a given IG or TR group, from NGS IMGT/HighV-QUEST statistical output. IMGT/StatClonotype tool is incorporated in the R package "IMGTStatClonotype," with a user-friendly interface. IMGT/StatClonotype is downloadable at IMGT(®) for users to evaluate pairwise comparison of IG and TR NGS statistical output from IMGT/HighV-QUEST and to visualize, on their web browser, the statistical significance of IMGT clonotype (AA) diversity or expression, per gene, the comparative analysis of CDR-IMGT and the V-D-J associations, in immunoprofiles from normal or pathological immune responses. PMID:27667992

  15. IMGT/StatClonotype for Pairwise Evaluation and Visualization of NGS IG and TR IMGT Clonotype (AA) Diversity or Expression from IMGT/HighV-QUEST

    Science.gov (United States)

    Aouinti, Safa; Giudicelli, Véronique; Duroux, Patrice; Malouche, Dhafer; Kossida, Sofia; Lefranc, Marie-Paule

    2016-01-01

    There is a huge need for standardized analysis and statistical procedures in order to compare the complex immune repertoires of antigen receptors immunoglobulins (IG) and T cell receptors (TR) obtained by next generation sequencing (NGS). NGS technologies generate millions of nucleotide sequences and have led to the development of new tools. The IMGT/HighV-QUEST, available since 2010, is the first global web portal for the analysis of IG and TR high throughput sequences. IMGT/HighV-QUEST provides standardized outputs for the characterization of the “IMGT clonotype (AA)” (AA for amino acids) and their comparison in up to one million sequences. Standardized statistical procedures for “IMGT clonotype (AA)” diversity or expression comparisons have recently been described, however, no tool was yet available. IMGT/StatClonotype, a new IMGT® tool, evaluates and visualizes statistical significance of pairwise comparisons of IMGT clonotype (AA) diversity or expression, per V (variable), D (diversity), and J (joining) gene of a given IG or TR group, from NGS IMGT/HighV-QUEST statistical output. IMGT/StatClonotype tool is incorporated in the R package “IMGTStatClonotype,” with a user-friendly interface. IMGT/StatClonotype is downloadable at IMGT®1 for users to evaluate pairwise comparison of IG and TR NGS statistical output from IMGT/HighV-QUEST and to visualize, on their web browser, the statistical significance of IMGT clonotype (AA) diversity or expression, per gene, the comparative analysis of CDR-IMGT and the V–D–J associations, in immunoprofiles from normal or pathological immune responses.

  16. IRSN annual report 2010

    International Nuclear Information System (INIS)

    IRSN, a public authority with industrial and commercial activities, was set up under Article 5 of French Act No. 2001-398 of May 9, 2001, enacted through Order No. 2002-254 of February 22, 2002. This Order was amended on April 7, 2007. The Institute is placed under the joint authority of the Ministries of Defense, the Environment, Industry, Research, and Health. It is the nation's public service expert in nuclear and radiation risks, and its activities cover all the related scientific and technical issues. Its areas of specialization include the environment and radiological emergency response, human radiation protection in both a medical and professional capacity, and in both normal and post-accident situations, the prevention of major accidents, nuclear reactor safety, as well as safety in plants and laboratories, transport and waste treatment, and nuclear defense expertise. IRSN interacts with all parties concerned by these risks (public authorities, in particular nuclear safety and security authorities, local authorities, companies, research organizations, stakeholders' associations, etc.) to contribute to public policy issues relating to nuclear safety, human and environmental protection against ionizing radiation, and the protection of nuclear materials, facilities, and transport against the risk of malicious acts. In this annual activity reports, IRSN presents the scientific and technical challenges that defines the Contract of Objectives (COB) signed between the Government and IRSN

  17. Annual Energy Review 1997

    Energy Technology Data Exchange (ETDEWEB)

    Seiferlein, Katherine E. [USDOE Energy Information Administration (EIA), Washington, DC (United States)

    1998-07-01

    The Annual Energy Review (AER) presents the Energy Information Administration’s historical energy statistics. For many series, statistics are given for every year from 1949 through 1997. The statistics, expressed in either physical units or British thermal units, cover all major energy activities, including consumption, production, trade, stocks, and prices, for all major energy commodities, including fossil fuels, electricity, and renewable energy sources. Publication of this report is in keeping with responsibilities given to the Energy Information Administration (EIA) in Public Law 95–91 (Department of Energy Organization Act), which states, in part, in Section 205(a)(2) that: “The Administrator shall be responsible for carrying out a central, comprehensive, and unified energy data and information program which will collect, evaluate, assemble, analyze, and disseminate data and information....” The AER is intended for use by Members of Congress, Federal and State agencies, energy analysts, and the general public. EIA welcomes suggestions from readers regarding data series in the AER and in other EIA publications.

  18. Annual Energy Review 2000

    Energy Technology Data Exchange (ETDEWEB)

    Seiferlein, Katherine E. [USDOE Energy Information Administration (EIA), Washington, DC (United States)

    2001-08-01

    The Annual Energy Review (AER) presents the Energy Information Administration’s historical energy statistics. For many series, statistics are given for every year from 1949 through 2000. The statistics, expressed in either physical units or British thermal units, cover all major energy activities, including consumption, production, trade, stocks, and prices, for all major energy commodities, including fossil fuels, electricity, and renewable energy sources. Publication of this report is required under Public Law 95–91 (Department of Energy Organization Act), Section 205(c), and is in keeping with responsibilities given to the Energy Information Administration under Section 205(a)(2), which states: “The Administrator shall be responsible for carrying out a central, comprehensive, and unified energy data and information program which will collect, evaluate, assemble, analyze, and disseminate data and information....” The AER is intended for use by Members of Congress, Federal and State agencies, energy analysts, and the general public. EIA welcomes suggestions from readers regarding data series in the AER and in other EIA publications.

  19. Annual report

    International Nuclear Information System (INIS)

    This is the thirty-ninth annual report of the Atomic Energy Control Board. The period covered by this report is the year ending March 31, 1986. The Atomic Energy Control Board (AECB) was established in 1946, by the Atomic Energy Control Act (AEC Act), (Revised Statues of Canada (R.S.C.) 1970 cA19). It is a departmental corporation (Schedule B) within the meaning and purpose of the Financial Administration Act. The AECB controls the development, application and use of atomic energy in Canada, and participates on behalf of Canada in international measures of control. The AECB is also repsonsible for the administration of the Nuclear Liability Act, (R.S.C. 1970 c29 1st Supp) as amended, including the designation of nuclear installations and the prescription of basic insurance to be carried by the operators of such nuclear installations. The AECB reports to Parliament through a designated Minister, currently the Minister of Energy, Mines and Resources

  20. FGFR2 mutation in a patient without typical features of Pfeiffer syndrome--The emerging role of combined NGS and phenotype based strategies.

    Science.gov (United States)

    Flöttmann, Ricarda; Knaus, Alexej; Zemojtel, Tomasz; Robinson, Peter N; Mundlos, Stefan; Horn, Denise; Spielmann, Malte

    2015-08-01

    Pfeiffer syndrome (MIM: #101600) is a rare autosomal dominant disorder classically characterized by limb and craniofacial anomalies. It is caused by heterozygous mutations in the fibroblast growth factor receptors types 1 and 2 (FGFR1 and FGFR2). We applied a next generation sequencing (NGS) panel approach comprising all 2877 genes currently known to be causative for one or more Mendelian diseases combined with the phenotype based computational tool PhenIX (Phenotypic Interpretation of eXomes). We report on a patient presenting with multiple anomalies of hands and feet including brachydactyly and symphalangism. No clinical diagnosis could be established based on the clinical findings and testing of several genes associated with brachydactyly and symphalangism failed to identify mutations. Via next generation sequencing (NGS) panel approach we then identified a novel de novo missense FGFR2 mutation affecting an amino acid reported to be mutated in Pfeiffer syndrome. Since our patient shows typical radiological findings of Pfeiffer syndrome in hands and feet but at the same time lacks several characteristic features such as clinical signs of craniosynostosis and prominent eyes we suggest introducing the term "FGFR2 associated phenotypes" for similar cases. Our results highlight the emerging role of combined NGS and phenotype based bioinformatics strategies to establish clinical diagnoses.

  1. Neurofibromatosis type 1 molecular diagnosis: what can NGS do for you when you have a large gene with loss of function mutations?

    Science.gov (United States)

    Pasmant, Eric; Parfait, Béatrice; Luscan, Armelle; Goussard, Philippe; Briand-Suleau, Audrey; Laurendeau, Ingrid; Fouveaut, Corinne; Leroy, Chrystel; Montadert, Annelore; Wolkenstein, Pierre; Vidaud, Michel; Vidaud, Dominique

    2015-05-01

    Molecular diagnosis of neurofibromatosis type 1 (NF1) is challenging owing to the large size of the tumour suppressor gene NF1, and the lack of mutation hotspots. A somatic alteration of the wild-type NF1 allele is observed in NF1-associated tumours. Genetic heterogeneity in NF1 was confirmed in patients with SPRED1 mutations. Here, we present a targeted next-generation sequencing (NGS) of NF1 and SPRED1 using a multiplex PCR approach (230 amplicons of ∼150 bp) on a PGM sequencer. The chip capacity allowed mixing 48 bar-coded samples in a 4-day workflow. We validated the NGS approach by retrospectively testing 30 NF1-mutated samples, and then prospectively analysed 279 patients in routine diagnosis. On average, 98.5% of all targeted bases were covered by at least 20X and 96% by at least 100X. An NF1 or SPRED1 alteration was found in 246/279 (88%) and 10/279 (4%) patients, respectively. Genotyping throughput was increased over 10 times, as compared with Sanger, with ∼90[euro ] for consumables per sample. Interestingly, our targeted NGS approach also provided quantitative information based on sequencing depth allowing identification of multiexons deletion or duplication. We then addressed the NF1 somatic mutation detection sensitivity in mosaic NF1 patients and tumours.

  2. Publicity and public relations

    Science.gov (United States)

    Fosha, Charles E.

    1990-01-01

    This paper addresses approaches to using publicity and public relations to meet the goals of the NASA Space Grant College. Methods universities and colleges can use to publicize space activities are presented.

  3. Photometric calibration of NGS/POSS and ESO/SRC plates using the NOAO PDS measuring engine. I - Stellar photometry

    Science.gov (United States)

    Cutri, Roc M.; Low, Frank J.; Marvel, Kevin B.

    1992-01-01

    The PDS/Monet measuring engine at the National Optical Astronomy Observatory was used to obtain photometry of nearly 10,000 stars on the NGS/POSS and 2000 stars on the ESO/SRC Survey glass plates. These measurements have been used to show that global transformation functions exist that allow calibration of stellar photometry from any blue or red plate to equivalent Johnson B and Cousins R photoelectric magnitudes. The four transformation functions appropriate for the POSS O and E and ESO/SRC J and R plates were characterized, and it was found that, within the measurement uncertainties, they vary from plate to plate only by photometric zero-point offsets. A method is described to correct for the zero-point shifts and to obtain calibrated B and R photometry of stellar sources to an average accuracy of 0.3-0.4 mag within the range R between values of 8 and 19.5 for red plates in both surveys, B between values of 9 and 20.5 on POSS blue plates, and B between values of 10 and 20.5 on ESO/SRC blue plates. This calibration procedure makes it possible to obtain rapid photometry of very large numbers of stellar sources.

  4. Assessment of the Impact of a New Guanidine Suppressor In NGS on F/H Laboratory Analyses For DWPF and Saltstone MCU Transfers

    International Nuclear Information System (INIS)

    Implementation of the Next Generation Solvent (NGS) in the Modular Caustic-Side Solvent Extraction Unit (MCU) will now proceed with a new suppressor compound, 1,2,3-tris(3,7-dimethyloctyl)guanidine (TiDG), replacing the originally planned suppressor for NGS, 1,3-dicyclohexyl-2-(11-methyldodecyl) guanidine (DCiTG). The Savannah River National Laboratory (SRNL) was tasked with evaluating the potential impact to F/H Laboratory analyses supporting the Defense Waste Processing Facility (DWPF) Waste Acceptance Criteria (WAC) used to qualify transfers of MCU Strip Effluent (SE) into the facility and the Saltstone WAC used to qualify transfers of Tank 50 containing Decontaminated Salt Solution (DSS) from MCU into Saltstone. This assigned scope is covered by a Task Technical and Quality Assurance Plan (TTQAP). Previous impact evaluations were conducted when the DCiTG suppressor was planned for NGS and concluded that there was no impact to either the determination of MCU SE pH nor the analysis of Isopar® L carryover in the MCU SE and DSS streams. SRNL reported on this series of cross-check studies between the SRNL and F/H Laboratories. The change in suppressor from DCiTG to TiDG in the NGS should not impact the measurement of Isopar® L or pH in SE or DSS necessary to satisfy DWPF and Saltstone WAC (Tank 50) criteria, respectively. A statistical study of the low bias observed in Isopar® L measurements in both SRNL and F/H Laboratories may be necessary now that the final NGS composition is fixed in order to quantify the low bias so that a proper correction can be applied to measurements critical to the DWPF and Saltstone WACs. Depending upon the final DWPF WAC requirement put in place for SE pH, it could become necessary to implement an alternative ICP-AES measurement of boron. The current blended solvent system testing in SRNL should address any impacts to Isopar® L carryover into either the DSS or the SE. It is recommended that SRNL monitor the current blended solvent

  5. Petroleum supply annual 1993. Volume 2

    Energy Technology Data Exchange (ETDEWEB)

    1994-06-01

    This publication contains information on the supply and disposition of crude oil and petroleum products. The publication reflects data that were collected from the petroleum industry during 1993 through annual and monthly surveys. This second volume contains final statistics for each month of 1993.

  6. Health and safety annual report 1989

    International Nuclear Information System (INIS)

    This 1989 annual report on Health and Safety in BNFL is intended to give the public a general review of the impact of the Company's activities on its workforce, the public and the environment. The activities at Sellafield, Springfields, Chapelcross, Drigg and Capenhurst are outlined, together with sections on medical services and transport, and radiation monitoring of workforce and the environment. (author)

  7. Identification and Characterization of Microsatellite Loci in Maqui (Aristotelia chilensis [Molina] Stunz) Using Next-Generation Sequencing (NGS).

    Science.gov (United States)

    Bastías, Adriana; Correa, Francisco; Rojas, Pamela; Almada, Rubén; Muñoz, Carlos; Sagredo, Boris

    2016-01-01

    Maqui (Aristotelia chilensis [Molina] Stunz) is a small dioecious tree native to South America with edible fruit characterized by very high antioxidant capacity and anthocyanin content. To preserve maqui as a genetic resource it is essential to study its genetic diversity. However, the complete genome is unknown and only a few gene sequences are available in databases. Simple sequence repeats (SSR) markers, which are neutral, co-dominant, reproducible and highly variable, are desirable to support genetic studies in maqui populations. By means of identification and characterization of microsatellite loci from a maqui genotype, using 454 sequencing technology, we develop a set of SSR for this species. Obtaining a total of 165,043 shotgun genome sequences, with an average read length of 387 bases, we covered 64 Mb of the maqui genome. Reads were assembled into 4,832 contigs, while 98,546 reads remained as singletons, generating a total of 103,378 consensus genomic sequences. A total of 24,494 SSR maqui markers were identified. Of them, 15,950 SSR maqui markers were classified as perfects. The most common SSR motifs were dinucleotide (31%), followed by tetranucleotide (26%) and trinucleotide motifs (24%). The motif AG/CT (28.4%) was the most abundant, while the motif AC (89 bp) was the largest. Eleven polymorphic SSRs were selected and used to analyze a population of 40 maqui genotypes. Polymorphism information content (PIC) ranged from 0.117 to 0.82, with an average of 0.58. Non-significant groups were observed in the maqui population, showing a panmictic genetic structure. In addition, we also predicted 11150 putative genes and 3 microRNAs (miRNAs) in maqui sequences. This results, including partial sequences of genes, some miRNAs and SSR markers from high throughput next generation sequencing (NGS) of maqui genomic DNA, constitute the first platform to undertake genetic and molecular studies of this important species. PMID:27459734

  8. Identification and Characterization of Microsatellite Loci in Maqui (Aristotelia chilensis [Molina] Stunz) Using Next-Generation Sequencing (NGS)

    Science.gov (United States)

    Bastías, Adriana; Correa, Francisco; Rojas, Pamela; Almada, Rubén; Muñoz, Carlos; Sagredo, Boris

    2016-01-01

    Maqui (Aristotelia chilensis [Molina] Stunz) is a small dioecious tree native to South America with edible fruit characterized by very high antioxidant capacity and anthocyanin content. To preserve maqui as a genetic resource it is essential to study its genetic diversity. However, the complete genome is unknown and only a few gene sequences are available in databases. Simple sequence repeats (SSR) markers, which are neutral, co-dominant, reproducible and highly variable, are desirable to support genetic studies in maqui populations. By means of identification and characterization of microsatellite loci from a maqui genotype, using 454 sequencing technology, we develop a set of SSR for this species. Obtaining a total of 165,043 shotgun genome sequences, with an average read length of 387 bases, we covered 64 Mb of the maqui genome. Reads were assembled into 4,832 contigs, while 98,546 reads remained as singletons, generating a total of 103,378 consensus genomic sequences. A total of 24,494 SSR maqui markers were identified. Of them, 15,950 SSR maqui markers were classified as perfects. The most common SSR motifs were dinucleotide (31%), followed by tetranucleotide (26%) and trinucleotide motifs (24%). The motif AG/CT (28.4%) was the most abundant, while the motif AC (89 bp) was the largest. Eleven polymorphic SSRs were selected and used to analyze a population of 40 maqui genotypes. Polymorphism information content (PIC) ranged from 0.117 to 0.82, with an average of 0.58. Non-significant groups were observed in the maqui population, showing a panmictic genetic structure. In addition, we also predicted 11150 putative genes and 3 microRNAs (miRNAs) in maqui sequences. This results, including partial sequences of genes, some miRNAs and SSR markers from high throughput next generation sequencing (NGS) of maqui genomic DNA, constitute the first platform to undertake genetic and molecular studies of this important species. PMID:27459734

  9. Annual report and accounts 1995

    International Nuclear Information System (INIS)

    This item consists of the Annual Report and Accounts of British Nuclear Fuels Limited (BNFL) for 1995 and a public relations document explaining the company's work in the United Kingdom nuclear power industry. BNFL has many years' experience in nuclear fuel manufacturing, reprocessing, waste management, decommissioning and transport of nuclear materials, and provides services to other organizations worldwide based on this expertise, making it an important export earner for the United Kingdom. (UK)

  10. Annual Report 2000-2001

    International Nuclear Information System (INIS)

    The annual report for the year 2000-2001 has been compiled, which offers concise description of tasks achieved and status of on going efforts pertaining to PAEC (Pakistan Atomic Commission) programme. The tasks description are as: highlights of various projects, nuclear power plants, physical sciences and engineering, biosciences, nuclear minerals, human resource development, projects, international relations. At the end of this report financial position of PAEC and list of publication is also available. (A.B.)

  11. Annual report 2003-2004

    International Nuclear Information System (INIS)

    The annual report for the year 2003-2004 has been compiled, which offers concise description of tasks achieved and status of on going efforts pertaining to PAEC (Pakistan Atomic Commission) programme. The tasks description are as: highlights of various projects, nuclear power plants, physical sciences and engineering, biosciences, nuclear minerals, human resource development, projects, international relations. At the end of this report financial position of PAEC and list of publication list is also available. (A.B.)

  12. DESY scientific annual report 1987

    International Nuclear Information System (INIS)

    This annual report contains a short description of the organization of DESY, extended abstracts concerning the experimental studies at the PETRA and DORIS storage rings, the development of the ZEUS detector, synchrotron-radiation experiments, theory of elementary particles, development of counting electronics, technical developments of the HERA, DORIS II and PETRA II storage rings and the DESY II respectively DESY I/III synchrotron, radiation protection, as well as data processing, and a list of speeches, publications, and theses. (HSI)

  13. Annual report 2008-09

    International Nuclear Information System (INIS)

    The Pakistan Atomic Energy Commission (PAEC) annual report for the year 2008-09 has been compiled. The salient features of the activities of various Centers, Power Plants and different project have been explained. The activities are described under the topics as: highlights of various projects, nuclear power, engineering, physical sciences, biological sciences, nuclear materials, safety, human resource development, PAEC health services projects and publications. (A.B).

  14. Annual report 2009-10

    International Nuclear Information System (INIS)

    The annual progress for the year 2009-10 for Pakistan Atomic Energy Commission (PAEC) is presented. The progress of various Center and their contribution for the uplift of nuclear programme have been described. The progress report fully explained under various topics as under: Highlights, Nuclear Power, Engineering, Physical Sciences, Biological Sciences, Nuclear Materials, Safety, Quality, Human Resource Development, PAEC General Health Services, Projects / International Collaboratioin and publications. (A.B.)

  15. 1996 Annual report

    International Nuclear Information System (INIS)

    This annual report present information of the main activities on the scope of the radiation protection and nuclear safety of the Nuclear Regulatory Authority (ARN) of the Argentina during 1996. The activities are developed in 10 chapters and 2 appendices, were are described the activities and the bases of the Argentine Regulatory System. The followings chapters and appendices are: 1.-Evolution of the nuclear regulatory activities in the Argentina since the beginnings in the CNEA. 2.-Regulatory system, laws and regulations. 3.-Institutional relations with national and international organizations. 4. and 5.-Safeguards and inspections of the nuclear installations. 6.-Radiation control of the occupational and public doses. 7.-Radiological emergencies. 8.-Projects and tasks scientific-technological. 9.-Training courses and meetings. 10.-Economic and human recourses. Appendix I.-Regulatory documentation. Appendix II.- Inspections of nuclear installations

  16. Uranium industry annual 1996

    International Nuclear Information System (INIS)

    The Uranium Industry Annual 1996 (UIA 1996) provides current statistical data on the US uranium industry's activities relating to uranium raw materials and uranium marketing. The UIA 1996 is prepared for use by the Congress, Federal and State agencies, the uranium and nuclear electric utility industries, and the public. Data on uranium raw materials activities for 1987 through 1996 including exploration activities and expenditures, EIA-estimated reserves, mine production of uranium, production of uranium concentrate, and industry employment are presented in Chapter 1. Data on uranium marketing activities for 1994 through 2006, including purchases of uranium and enrichment services, enrichment feed deliveries, uranium fuel assemblies, filled and unfilled market requirements, uranium imports and exports, and uranium inventories are shown in Chapter 2. A feature article, The Role of Thorium in Nuclear Energy, is included. 24 figs., 56 tabs

  17. Annual report for 1980

    International Nuclear Information System (INIS)

    This annual report contains extended abstracts of the work done in the named Laboratory together with a list of publications and reports. The abstracts concern deep-inelastic and transfer reactions, compound-nucleus reactions including fusion and fission, reactions with light and with polarized particles, gamma-ray spectroscopy, and coulomb excitation, atomic physics, the irradiation of biological systems, nuclear structure, nuclear reaction theory, dissipative reactions, accelerator developments, the superconducting sector-cyclotron SuSe, the synchrotron-radiation source Little Erna, detectors, technology, the on-line computer system, the online-offline programming system GOOPSY, the megachannel analyzer for the acquisition of multidimensional events MADAME, and the central monitoring and control system ZUeSS. (HSI)

  18. Annual Report 2010

    International Nuclear Information System (INIS)

    The Annual Report of the Institute of Nuclear Chemistry and Technology (Warsaw, Poland) describes achievements of the Institute in 2010 obtained by three Centres and eight Laboratories, namely: (1) Centre for Radiation Research and Technology, (2) Centre of Radiochemistry and Nuclear Chemistry, (3) Centre of Radiobiology and Biological Dosimetry, (4) Laboratory of Nuclear Analytical Methods, (5) Laboratory of Material Research, (6) Pollution Control Technologies Laboratory, (7) Laboratory of Stable and Environmental Isotopes, (8) Laboratory for Measurements of Technological Doses, (9) Laboratory for Detection of Irradiated Food and (10) Laboratory of Nuclear Control Systems and Methods. In total - 32 detailed papers prepared by the Institute workers and collaborating scientists are presented. General information on the Institute status, personnel activity, international cooperation and publications are presented.

  19. SKB annual report 1987

    International Nuclear Information System (INIS)

    The annual report on the activities of the Swedish Nuclear Fuel and Waste Management contains in part I an overview of SKB activities in different fields. Part II gives a description of the research and development work on nuclear waste disposal performed during 1987. Lectures and publications during 1987 as well as reports issued in the SKB technical report series are listed in part III. Part IV contains the summaries of all technical reports issued during 1987. At Forsmark the first construction phase for the final repository for radioactive waste - SFR - is now completed. The repository is situated in crystalline rock under the Baltic Sea. The first construction phase includes rock caverns for 60 000 m3 of waste. A second phase for additional 30 000 m3 is planned to be built and commissioned around the year 2000. (orig./DG)

  20. Uranium Industry Annual, 1992

    International Nuclear Information System (INIS)

    The Uranium Industry Annual provides current statistical data on the US uranium industry for the Congress, Federal and State agencies, the uranium and electric utility industries, and the public. The feature article, ''Decommissioning of US Conventional Uranium Production Centers,'' is included. Data on uranium raw materials activities including exploration activities and expenditures, resources and reserves, mine production of uranium, production of uranium concentrate, and industry employment are presented in Chapter 1. Data on uranium marketing activities including domestic uranium purchases, commitments by utilities, procurement arrangements, uranium imports under purchase contracts and exports, deliveries to enrichment suppliers, inventories, secondary market activities, utility market requirements, and uranium for sale by domestic suppliers are presented in Chapter 2

  1. Annual report 1982

    International Nuclear Information System (INIS)

    This annual report gives a survey of the activities of ECN at The Hague and Petten, Netherlands, in 1982. These activities are concerned with energy generation and development and with scientific and technical applications of thermal neutrons, which are available from the High Flux Reactor and the Low Flux Reactor at Petten. The Energy Study Centre (ESC), a special department of ECN, is engaged with social-economic studies on energy generation and utilization. ESC also investigates the consequences of energy scenarios. The Bureau Energy Research Projects (BEOP) coordinates and administers all national research projects, especially on flywheels, solar energy, wind power and coal combustion. After a survey of staffing and finances the report ends with a list of ECN publications

  2. Annual report 1999

    International Nuclear Information System (INIS)

    This 1999 annual report of the Authorities of Nuclear Safety presents the main technical topics of the year: time evolution of nuclear installations, crisis and particular plan of intervention, impacts of the nuclear activities on the human and the environment, the criticality risk, EdF, the EPR project, the ANDRA, transportation events and the nuclear safety at the East. It provides also information on nuclear safety, organization of nuclear safety supervision, BIN regulatory provisions, plant supervision, information of the public, international relations, emergency response, radiation protection, radioactive material transportation, radioactive waste, PWR reactors, research reactors and other installations, nuclear fuel cycle installations and final shutdown and dismantling of nuclear installations. (A.L.B.)

  3. NEA annual report 2000

    International Nuclear Information System (INIS)

    In this 2000 annual report, the Nuclear Energy Agency (NEA), notes that in the medium and long term, the evolution of the nuclear energy programs in OECD countries is likely to be influenced by the implementation of sustainable development policies integrating economic, environmental and socials goals. This report is divided in four chapters. The first one gives general information on nuclear industry. The chapter 2 deals with trends in nuclear power. The chapter 3 gathers technical programs in nuclear development and fuel cycle, nuclear safety and regulation, radiation protection, radioactive waste management, nuclear science and data bank, legal affairs, joint projects and other co-operative projects. The last chapter gathers general information on information program, NEA publications, main workshops and seminar and organisation charts of the NEA. (A.L.B.)

  4. Annual Report 2009

    International Nuclear Information System (INIS)

    The Annual Report of the Institute of Nuclear Chemistry and Technology (Warsaw, Poland) describes achievements of the Institute in 2009 obtained in ten laboratories: (1) Centre for Radiation Research and Technology, (2) Centre of Radiochemistry and Nuclear Chemistry, (3) Centre of Radiobiology and Biological Dosimetry, (4) Laboratory of Nuclear Analytical Methods, (5) Laboratory of Material Research, (6) Pollution Control Technologies Laboratory, (7) Laboratory of Stable and Environmental Isotopes, (8) Laboratory for Measurements of Technological Doses, (9) Laboratory for Detection of Irradiated Food, (10) Laboratory of Nuclear Control Systems and Methods. In total - 49 detailed papers prepared by the Institute staff and collaborating scientists are presented. General information on the Institute status, personnel activity, international cooperation and publications are also given.

  5. Uranium industry annual 1996

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-04-01

    The Uranium Industry Annual 1996 (UIA 1996) provides current statistical data on the US uranium industry`s activities relating to uranium raw materials and uranium marketing. The UIA 1996 is prepared for use by the Congress, Federal and State agencies, the uranium and nuclear electric utility industries, and the public. Data on uranium raw materials activities for 1987 through 1996 including exploration activities and expenditures, EIA-estimated reserves, mine production of uranium, production of uranium concentrate, and industry employment are presented in Chapter 1. Data on uranium marketing activities for 1994 through 2006, including purchases of uranium and enrichment services, enrichment feed deliveries, uranium fuel assemblies, filled and unfilled market requirements, uranium imports and exports, and uranium inventories are shown in Chapter 2. A feature article, The Role of Thorium in Nuclear Energy, is included. 24 figs., 56 tabs.

  6. 1997 Annual report

    International Nuclear Information System (INIS)

    This annual report present information of the main activities on the scope of the radiation protection and nuclear safety of the Nuclear Regulatory Authority (ARN) of the Argentina during 1997. The activities are developed in 11 chapters and 1 appendix, were are descripting the activities and the bases of the Argentine Regulatory System. The following chapters and the appendix are: 1.-Evolution of the nuclear regulatory activities in the Argentina. 2.-Regulatory system, laws and regulations. 3.-Institutional relations with national and international organizations. 4.-Inspections and evaluations of the nuclear reactors. 5.-Inspections and evaluations of the radioactive facilities. 6.-Inspections and evaluations of safeguards and physical protection. 7.-Radiation control of the occupational and public doses. 8.-Radiological emergencies. 9.-Projects and tasks scientific-technological. 10.-Training courses and meetings. 11.-Economic and human recourses. Appendix.- Inspections of radioactive facilities

  7. Annual report 1993

    International Nuclear Information System (INIS)

    The book is a collection of short communications being a review of the scientific activity of Institute of Nuclear Chemistry and Technology, Warsaw in 1993. The papers are gathered in several branches as follows: radiation chemistry and physics (9); radiochemistry, stable isotopes, nuclear analytical methods, general chemistry (22); radiobiology (6). The last biggest chapter devoted to nuclear technologies and methods is divided into four smaller groups concerning: process engineering (2); material engineering, structural studies, diagnostics (14); radiation technologies (8) and nuclear control systems (4). The annual report of INCT 1993 contains also a general information about the staff and organizations of the Institute, the full list of scientific publications and patents, conferences organized by INCT, Ph.D. and Sc.D. thesis and a list of projects granted by Committee of Scientific Research and other organizations in Poland and elsewhere

  8. 2012 NEA Annual Report

    International Nuclear Information System (INIS)

    After a message from the Director-General of the Nuclear Energy Agency (NEA, a semi-autonomous body within the OECD) and an overview of the Fukushima accident and NEA follow-up, this annual report presents the various technical programmes in which the NEA is committed. These programmes deal with nuclear development and the fuel cycle, nuclear safety and regulation, radioactive waste management, radiological protection, nuclear science, data bank, legal affairs, joint projects and other cooperative projects. The report then presents activities of the technical secretariat (Generation IV International Forum or GIF, Multinational Design Evaluation Programme or MDEP), and some general information about the NEA (organisational structure, publications in 2012) and about its activities of information and communication activities, and regarding the relationship between nuclear energy and civil society

  9. SKB annual report 1988

    International Nuclear Information System (INIS)

    This is the annual report on the activities of the Swedish Nuclear Fuel and Waste Management Co, SKB. It contains in part 1 an overview of SKB activities in different fields. Part 2 gives a description of the research and development work on nuclear waste disposal performed during 1988. Lectures and publications during 1988 as well as reports issued in the SKB technical report series are listed in part 3. Part 4 contains the summaries of all technical reports issued during 1988. SKB is in charge of a comprehensive research and development program on geological disposal of nuclear waste. The total cost for R and D during 1988 was 123.4 MSEK of which 19.3 MSEK came from participants outside Sweden

  10. Uranium Industry Annual, 1992

    Energy Technology Data Exchange (ETDEWEB)

    1993-10-28

    The Uranium Industry Annual provides current statistical data on the US uranium industry for the Congress, Federal and State agencies, the uranium and electric utility industries, and the public. The feature article, ``Decommissioning of US Conventional Uranium Production Centers,`` is included. Data on uranium raw materials activities including exploration activities and expenditures, resources and reserves, mine production of uranium, production of uranium concentrate, and industry employment are presented in Chapter 1. Data on uranium marketing activities including domestic uranium purchases, commitments by utilities, procurement arrangements, uranium imports under purchase contracts and exports, deliveries to enrichment suppliers, inventories, secondary market activities, utility market requirements, and uranium for sale by domestic suppliers are presented in Chapter 2.

  11. GKSS annual report 1989

    International Nuclear Information System (INIS)

    GKSS-Forschungszentrum Geesthacht GmbH, as a national research center, carries out development work on behalf of the Federal Government's research and technology policy in order to deepen scientific knowledge, conserve resources and environment, improve living and working conditions, and increase economic efficiency and competitiveness. The center carries out work on energy research and energy technology, basic technologies, marine research and techniques, health-environment-biotechnology. The annual report contains in summary selected research work, the report on R and D activities in reactor safety, materials, environment and climate, underwater and environmental techniques. The research institutes, cooperation with external partners, the organization, budget, personnel, publications, including patent applications, and lectures are described. (HK)

  12. Public relations

    International Nuclear Information System (INIS)

    At Nuclear Regulatory Authority of the Slovak Republic (NRA SR), the public relations belongs to the secretariat of the Chairman, and are a part of the policy of carefully planned and purposeful efforts to establish mutual relations between the authority and the public. A spokesmen of NRA SR is in charge of the public relations. The spokesman is ready, without a useless filibuster and based on a particular requirement, to inform governmental bodies, other national bodies and organizations, embassies and international organizations, the public and news media in case of an event at a nuclear installation. To provide for communications activities, NRA SR constructed and opened the Information centre with a particular equipment in autumn 1995, that has already started communications with some dailies, broadcasting, television and Press Agency SR. It has been envisaged that there will be press conferences held in the Information centre a few times a year, or NRA SR senior staff may be interviewed here on extraordinary events at NPPs, or on some other important occasions in NRA SR. In 1995, NRA Sr issued the Annual report in a few variants - each suitable for different use - on NRA SR activities and nuclear safety of Slovak nuclear power plants as of 1994. The NRA SR's Bulletin has started to be published with periodicity of 3 times a year, focusing on NRA SR activities both in Slovakia and abroad. NRA SR Information centre provides foreign visitors with independent propagation and information materials about the issue of nuclear safety enhancement at operational Slovak NPPs. Furthermore, the Information centre provides both the NRA SR's residences in Bratislava and Trnava with daily press monitoring of topical news

  13. Public Education, Public Good.

    Science.gov (United States)

    Tomlinson, John

    1986-01-01

    Criticizes policies which would damage or destroy a public education system. Examines the relationship between government-provided education and democracy. Concludes that privatization of public education would emphasize self-interest and selfishness, further jeopardizing the altruism and civic mindedness necessary for the public good. (JDH)

  14. Public entrepreneurship as innovative management strategy in the public sector : a public choice-approach

    OpenAIRE

    Mierlo, J.G.A. van

    1996-01-01

    Paper Originally Presented at the 65th Annual Conference of the Southern Economic Association , Fairmont Hotel, New Orleans, Louisiana, United States of America, November 18-20, 1995. Bureaucratic service organisations in the public sector are increasingly loosing their previous comfortable monopoly positions in providing services to the public, due to radical structural changes in modern society. The public finance of their services by politicians ordering public services as elected represen...

  15. Annual report and accounts 1991-92

    International Nuclear Information System (INIS)

    The 1991-1992 Annual Report of Scottish Nuclear Limited includes a brief company profile. Scottish Nuclear, a company owned by the Secretary of State for Scotland, produces over forty per cent of Scotland's total electricity requirement from its Advanced Gas-Cooled Reactors at Hunterston and Torness. The Chairman's Report focuses an operational performance, improvements to public relations procedures and environmental concerns. The Report concludes with the Annual Accounts. (UK)

  16. NGS-based BRCA1/2 mutation testing of high-grade serous ovarian cancer tissue: results and conclusions of the first international round robin trial.

    Science.gov (United States)

    Endris, Volker; Stenzinger, Albrecht; Pfarr, Nicole; Penzel, Roland; Möbs, Markus; Lenze, Dido; Darb-Esfahani, Silvia; Hummel, Michael; Sabine-Merkelbach-Bruse; Jung, Andreas; Lehmann, Ulrich; Kreipe, Hans; Kirchner, Thomas; Büttner, Reinhard; Jochum, Wolfram; Höfler, Gerald; Dietel, Manfred; Weichert, Wilko; Schirmacher, Peter

    2016-06-01

    With the approval of olaparib as monotherapy treatment in platinum-sensitive, relapsed high-grade serous ovarian cancer by the European Medical Agency (EMA), comprehensive genotyping of BRCA1 and BRCA2 in tumor tissue has become a mandatory pre-therapeutic test. This requires significant advances in routine tumor test methodologies due to the large size of both genes and the lack of mutational hot spots. Classical focused screening approaches, like Sanger sequencing, do not allow for a sensitive, rapid, and economic analysis of tumor tissue. Next-generation sequencing (NGS) approaches employing targeted panels for BRCA1/2 to interrogate formalin-fixed and paraffin-embedded tumor samples from either surgical resection or biopsy specimens can overcome these limitations. Although focused NGS methods have been implemented by few centers in routine molecular diagnostics for the analysis of some druggable oncogenic mutations, the reliable diagnostic testing of the entire coding regions of BRCA1 and BRCA2 was a new challenge requiring extensive technological improvement and quality management. Here, we describe the implementation and results of the first round robin trial for BRCA1/2 mutation testing in tumor tissue that was conducted in central Europe on May 2015, shortly after the approval and prior to the official release of olaparib. The high success rate of 81 % (21/26 test centers) demonstrates that BRCA1/2 multicenter mutation testing is well feasible in FFPE tumor tissue, extending to other tumor entities beyond ovarian cancer. The high number of test centers passing the trial demonstrates the success of the concerted efforts by German, Swiss, and Austrian pathology centers to ensure quality-controlled NGS-based testing and proves the potential of this technology in routine molecular pathology. On the basis of our results, we provide recommendations for predictive testing of tumor tissue for BRCA1/2 to clinical decision making in ovarian cancer patients.

  17. Petroleum supply annual 1998: Volume 1

    International Nuclear Information System (INIS)

    The ''Petroleum Supply Annual'' (PSA) contains information on the supply and disposition of crude oil and petroleum products. The publication reflects data that were collected from the petroleum industry during 1998 through annual and monthly surveys. The PSA is divided into two volumes. This first volume contains three sections: Summary Statistics, Detailed Statistics, and Refinery Statistics; each with final annual data. The second volume contains final statistics for each month of 1998, and replaces data previously published in the PSA. The tables in Volumes 1 and 2 are similarly numbered to facilitate comparison between them. 16 figs., 59 tabs

  18. Petroleum supply annual 1994. Volume 1

    International Nuclear Information System (INIS)

    The Petroleum Supply Annual (PSA) contains information on the supply and disposition of crude oil and petroleum products. The publication reflects data that were collected from the petroleum industry during 1994 through annual and monthly surveys. The PSA is divided into two volumes. This first volume contains four sections: Summary Statistics, Detailed Statistics, Refinery Capacity, and Oxygenate Capacity each with final annual data. The second volume contains final statistics for each month of 1994, and replaces data previously published in the Petroleum Supply Monthly (PSM). The tables in Volumes 1 and 2 are similarly numbered to facilitate comparison between them. Below is a description of each section in Volume 1 of the PSA

  19. Petroleum supply annual, 1997. Volume 1

    International Nuclear Information System (INIS)

    The Petroleum Supply Annual (PSA) contains information on the supply and disposition of crude oil and petroleum products. The publication reflects data that were collected from the petroleum industry during 1997 through annual and monthly surveys. The PSA is divided into two volumes. This first volume contains three sections: Summary Statistics, Detailed Statistics, and Refinery Statistics; each with final annual data. The second volume contains final statistics for each month of 1997, and replaces data previously published in the Petroleum Supply Monthly (PSM). The tables in Volumes 1 and 2 are similarly numbered to facilitate comparison between them. 16 figs., 48 tabs

  20. Petroleum supply annual 1994. Volume 1

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-05-22

    The Petroleum Supply Annual (PSA) contains information on the supply and disposition of crude oil and petroleum products. The publication reflects data that were collected from the petroleum industry during 1994 through annual and monthly surveys. The PSA is divided into two volumes. This first volume contains four sections: Summary Statistics, Detailed Statistics, Refinery Capacity, and Oxygenate Capacity each with final annual data. The second volume contains final statistics for each month of 1994, and replaces data previously published in the Petroleum Supply Monthly (PSM). The tables in Volumes 1 and 2 are similarly numbered to facilitate comparison between them. Below is a description of each section in Volume 1 of the PSA.

  1. Petroleum supply annual 1992: Volume 1

    International Nuclear Information System (INIS)

    The Petroleum Supply Annual (PSA) contains information on the supply and disposition of crude oil and petroleum products. The publication reflects data that were collected from the petroleum industry during 1992 through annual and monthly surveys. The PSA is divided into two volumes. This first volume contains four sections: Summary Statistics, Detailed Statistics, Refinery Capacity and Oxygenate Capacity each with final annual data. The second volume contains final statistics for each month of 1992, and replaces data previously published in the Petroleum Supply Monthly (PSM). The tables in Volumes 1 and 2 are similarly numbered to facilitate comparison between them

  2. Petroleum supply annual 1993. Volume 1

    Energy Technology Data Exchange (ETDEWEB)

    1994-06-01

    The Petroleum Supply Annual (PSA) contains information on the supply and disposition of crude oil and petroleum products. The publication reflects data that were collected from the petroleum industry during 1993 through annual and monthly surveys. The PSA is divided into two volumes. This first volume contains four sections: Summary Statistics, Detailed Statistics, Refinery Capacity, and Oxygenate Capacity each with final annual data. The second volume contains final statistics for each month of 1993, and replaces data previously published in the Petroleum Supply Monthly (PSM). The tables in Volumes 1 and 2 are similarly numbered to facilitate comparison between them. Below is a description of each section in Volume 1 of the PSA.

  3. Petroleum supply annual, 1997. Volume 1

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-06-01

    The Petroleum Supply Annual (PSA) contains information on the supply and disposition of crude oil and petroleum products. The publication reflects data that were collected from the petroleum industry during 1997 through annual and monthly surveys. The PSA is divided into two volumes. This first volume contains three sections: Summary Statistics, Detailed Statistics, and Refinery Statistics; each with final annual data. The second volume contains final statistics for each month of 1997, and replaces data previously published in the Petroleum Supply Monthly (PSM). The tables in Volumes 1 and 2 are similarly numbered to facilitate comparison between them. 16 figs., 48 tabs.

  4. Petroleum supply annual 1998: Volume 1

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1999-06-01

    The ``Petroleum Supply Annual`` (PSA) contains information on the supply and disposition of crude oil and petroleum products. The publication reflects data that were collected from the petroleum industry during 1998 through annual and monthly surveys. The PSA is divided into two volumes. This first volume contains three sections: Summary Statistics, Detailed Statistics, and Refinery Statistics; each with final annual data. The second volume contains final statistics for each month of 1998, and replaces data previously published in the PSA. The tables in Volumes 1 and 2 are similarly numbered to facilitate comparison between them. 16 figs., 59 tabs.

  5. Caracterización de la microbiota intestinal mediante "NGS" en patología neoplásica de colón

    OpenAIRE

    Galán Ros, Jorge

    2015-01-01

    Objetivos: Nuestro objetivo consistió en caracterizar, mediante secuenciación de próxima generación (NGS), la microbiota colónica en muestras de mucosa normal y tumoral de pacientes con cáncer colorrectal (CCR), así como en muestras de heces de pacientes sanos y enfermos, además de estudiar las diferencias en la composición microbiana según el tipo, estadío y localización tumoral, con el fin de sugerir qué tipo de bacterias podrían estar previsiblemente involucradas en la localización, desarr...

  6. Exploring the Genes of Yerba Mate (Ilex paraguariensis A. St.-Hil.) by NGS and De Novo Transcriptome Assembly

    Science.gov (United States)

    Aguilera, Patricia M.; Bubillo, Rosana E.; Otegui, Mónica B.; Ducasse, Daniel A.; Zapata, Pedro D.; Marti, Dardo A.

    2014-01-01

    Yerba mate (Ilex paraguariensis A. St.-Hil.) is an important subtropical tree crop cultivated on 326,000 ha in Argentina, Brazil and Paraguay, with a total yield production of more than 1,000,000 t. Yerba mate presents a strong limitation regarding sequence information. The NCBI GenBank lacks an EST database of yerba mate and depicts only 80 DNA sequences, mostly uncharacterized. In this scenario, in order to elucidate the yerba mate gene landscape by means of NGS, we explored and discovered a vast collection of I. paraguariensis transcripts. Total RNA from I. paraguariensis was sequenced by Illumina HiSeq-2000 obtaining 72,031,388 pair-end 100 bp sequences. High quality reads were de novo assembled into 44,907 transcripts encompassing 40 million bases with an estimated coverage of 180X. Multiple sequence analysis allowed us to predict that yerba mate contains ∼32,355 genes and 12,551 gene variants or isoforms. We identified and categorized members of more than 100 metabolic pathways. Overall, we have identified ∼1,000 putative transcription factors, genes involved in heat and oxidative stress, pathogen response, as well as disease resistance and hormone response. We have also identified, based in sequence homology searches, novel transcripts related to osmotic, drought, salinity and cold stress, senescence and early flowering. We have also pinpointed several members of the gene silencing pathway, and characterized the silencing effector Argonaute1. We predicted a diverse supply of putative microRNA precursors involved in developmental processes. We present here the first draft of the transcribed genomes of the yerba mate chloroplast and mitochondrion. The putative sequence and predicted structure of the caffeine synthase of yerba mate is presented. Moreover, we provide a collection of over 10,800 SSR accessible to the scientific community interested in yerba mate genetic improvement. This contribution broadly expands the limited knowledge of yerba mate genes

  7. Exploring the genes of yerba mate (Ilex paraguariensis A. St.-Hil. by NGS and de novo transcriptome assembly.

    Directory of Open Access Journals (Sweden)

    Humberto J Debat

    Full Text Available Yerba mate (Ilex paraguariensis A. St.-Hil. is an important subtropical tree crop cultivated on 326,000 ha in Argentina, Brazil and Paraguay, with a total yield production of more than 1,000,000 t. Yerba mate presents a strong limitation regarding sequence information. The NCBI GenBank lacks an EST database of yerba mate and depicts only 80 DNA sequences, mostly uncharacterized. In this scenario, in order to elucidate the yerba mate gene landscape by means of NGS, we explored and discovered a vast collection of I. paraguariensis transcripts. Total RNA from I. paraguariensis was sequenced by Illumina HiSeq-2000 obtaining 72,031,388 pair-end 100 bp sequences. High quality reads were de novo assembled into 44,907 transcripts encompassing 40 million bases with an estimated coverage of 180X. Multiple sequence analysis allowed us to predict that yerba mate contains ∼ 32,355 genes and 12,551 gene variants or isoforms. We identified and categorized members of more than 100 metabolic pathways. Overall, we have identified ∼ 1,000 putative transcription factors, genes involved in heat and oxidative stress, pathogen response, as well as disease resistance and hormone response. We have also identified, based in sequence homology searches, novel transcripts related to osmotic, drought, salinity and cold stress, senescence and early flowering. We have also pinpointed several members of the gene silencing pathway, and characterized the silencing effector Argonaute1. We predicted a diverse supply of putative microRNA precursors involved in developmental processes. We present here the first draft of the transcribed genomes of the yerba mate chloroplast and mitochondrion. The putative sequence and predicted structure of the caffeine synthase of yerba mate is presented. Moreover, we provide a collection of over 10,800 SSR accessible to the scientific community interested in yerba mate genetic improvement. This contribution broadly expands the limited knowledge

  8. Science, Technology, and American Diplomacy 1985. Sixth Annual Report Submitted to the Congress by the President Pursuant to Section 503(b) of Title V of Public Law 95-426. Serial E.

    Science.gov (United States)

    Department of State, Washington, DC.

    The Reagan administration's annual report to the Congress on international activities in the fields of science and technology (S&T) for fiscal year 1984 consists of three parts. The two chapters in part I (S&T in American diplomacy) examine S&T in American foreign policy and resources necessary for successful diplomacy. The two chapters in part II…

  9. Bruce NGS rehabilitation program

    International Nuclear Information System (INIS)

    The paper summarizes the status at the time of writing of the program to rehabilitate the 4-unit Bruce A Nuclear Generating Station. The aim of the program was to restore the target 85% capacity factor by repair and proactive maintenance

  10. SKB annual report 1992

    International Nuclear Information System (INIS)

    This is the annual report on the activities of the Swedish Nuclear Fuel and Waste Management Co, SKB. It contains in part 1 an overview of SKB activities in different fields. Part 2 gives a description of the research and development work on nuclear waste disposal performed during 1992. Lectures and publications during 1992 as well as reports issued in the SKB technical report series are listed in part 4. Part 5 contains the summaries of all technical reports issued during 1992. SKB is the owner of CLAB, the Central Facility for Interim Storage of Spent Nuclear Fuel, located at Oskarshamn. CLAB was taken into operation in July 1985 and to the end of 1992 in total 1684 tonnes of spent fuel (measured as uranium) has been received. Transportation from the nuclear site to CLAB is made by a special ship, M/S Sigyn. At Forsmark the final repository for Radioactive Waste -SFR- was taken in operation in April 1988. At the end of 1992 a total of 11000 m3 of waste have been deposited in SFR. The total cost for R and D during 1992 was 192.3 MSEK of which 24.8 MSEK came from participants outside Sweden. Some of the main areas for SKB research are: groundwater movements, bedrock stability, groundwater chemistry and nuclide migration, method and instruments for in situ characterization of crystalline bedrock, characterization and leaching of spent nuclear fuel, properties of bentonite for buffer, backfilling and sealing, radionuclide transport in biosphere and dose evaluations, development of performance and safety assessment methodology and assessment models, construction of an underground research laboratory. Cost calculations for the total nuclear waste management system, including decommissioning of all reactors, are updated annually. The total cost is estimated to 55 billion SEK

  11. Trends in IT Innovation to Build a Next Generation Bioinformatics Solution to Manage and Analyse Biological Big Data Produced by NGS Technologies.

    Science.gov (United States)

    de Brevern, Alexandre G; Meyniel, Jean-Philippe; Fairhead, Cécile; Neuvéglise, Cécile; Malpertuy, Alain

    2015-01-01

    Sequencing the human genome began in 1994, and 10 years of work were necessary in order to provide a nearly complete sequence. Nowadays, NGS technologies allow sequencing of a whole human genome in a few days. This deluge of data challenges scientists in many ways, as they are faced with data management issues and analysis and visualization drawbacks due to the limitations of current bioinformatics tools. In this paper, we describe how the NGS Big Data revolution changes the way of managing and analysing data. We present how biologists are confronted with abundance of methods, tools, and data formats. To overcome these problems, focus on Big Data Information Technology innovations from web and business intelligence. We underline the interest of NoSQL databases, which are much more efficient than relational databases. Since Big Data leads to the loss of interactivity with data during analysis due to high processing time, we describe solutions from the Business Intelligence that allow one to regain interactivity whatever the volume of data is. We illustrate this point with a focus on the Amadea platform. Finally, we discuss visualization challenges posed by Big Data and present the latest innovations with JavaScript graphic libraries. PMID:26125026

  12. Next-generation sequencing (NGS) as a fast molecular diagnosis tool for left ventricular noncompaction in an infant with compound mutations in the MYBPC3 gene.

    Science.gov (United States)

    Schaefer, Elise; Helms, Pauline; Marcellin, Luc; Desprez, Philippe; Billaud, Philippe; Chanavat, Valérie; Rousson, Robert; Millat, Gilles

    2014-03-01

    Left ventricular noncompaction (LVNC) is a clinically heterogeneous disorder characterized by a trabecular meshwork and deep intertrabecular myocardial recesses that communicate with the left ventricular cavity. LVNC is classified as a rare genetic cardiomyopathy. Molecular diagnosis is a challenge for the medical community as the condition shares morphologic features of hypertrophic and dilated cardiomyopathies. Several genetic causes of LVNC have been reported, with variable modes of inheritance, including autosomal dominant and X-linked inheritance, but relatively few responsible genes have been identified. In this report, we describe a case of a severe form of LVNC leading to death at 6 months of life. NGS sequencing using a custom design for hypertrophic cardiomyopathy panel allowed us to identify compound heterozygosity in the MYBPC3 gene (p.Lys505del, p.Pro955fs) in 3 days, confirming NGS sequencing as a fast molecular diagnosis tool. Other studies have reported neonatal presentation of cardiomyopathies associated with compound heterozygous or homozygous MYBPC3 mutations. In this family and in families in which parental truncating MYBPC3 mutations are identified, preimplantation or prenatal genetic screening should be considered as these genotypes leads to neonatal mortality and morbidity.

  13. Impact of NGS in the medical sciences: Genetic syndromes with an increased risk of developing cancer as an example of the use of new technologies.

    Science.gov (United States)

    Lapunzina, Pablo; López, Rocío Ortiz; Rodríguez-Laguna, Lara; García-Miguel, Purificación; Martínez, Augusto Rojas; Martínez-Glez, Víctor

    2014-03-01

    The increased speed and decreasing cost of sequencing, along with an understanding of the clinical relevance of emerging information for patient management, has led to an explosion of potential applications in healthcare. Currently, SNP arrays and Next-Generation Sequencing (NGS) technologies are relatively new techniques used to scan genomes for gains and losses, losses of heterozygosity (LOH), SNPs, and indel variants as well as to perform complete sequencing of a panel of candidate genes, the entire exome (whole exome sequencing) or even the whole genome. As a result, these new high-throughput technologies have facilitated progress in the understanding and diagnosis of genetic syndromes and cancers, two disorders traditionally considered to be separate diseases but that can share causal genetic alterations in a group of developmental disorders associated with congenital malformations and cancer risk. The purpose of this work is to review these syndromes as an example of a group of disorders that has been included in a panel of genes for NGS analysis. We also highlight the relationship between development and cancer and underline the connections between these syndromes.

  14. Trends in IT Innovation to Build a Next Generation Bioinformatics Solution to Manage and Analyse Biological Big Data Produced by NGS Technologies

    Directory of Open Access Journals (Sweden)

    Alexandre G. de Brevern

    2015-01-01

    Full Text Available Sequencing the human genome began in 1994, and 10 years of work were necessary in order to provide a nearly complete sequence. Nowadays, NGS technologies allow sequencing of a whole human genome in a few days. This deluge of data challenges scientists in many ways, as they are faced with data management issues and analysis and visualization drawbacks due to the limitations of current bioinformatics tools. In this paper, we describe how the NGS Big Data revolution changes the way of managing and analysing data. We present how biologists are confronted with abundance of methods, tools, and data formats. To overcome these problems, focus on Big Data Information Technology innovations from web and business intelligence. We underline the interest of NoSQL databases, which are much more efficient than relational databases. Since Big Data leads to the loss of interactivity with data during analysis due to high processing time, we describe solutions from the Business Intelligence that allow one to regain interactivity whatever the volume of data is. We illustrate this point with a focus on the Amadea platform. Finally, we discuss visualization challenges posed by Big Data and present the latest innovations with JavaScript graphic libraries.

  15. Annual Check-up

    Science.gov (United States)

    ... Medical Conditions Nutrition & Fitness Emotional Health Annual Check-Up Posted under Health Guides . Updated 7 January 2015. + ... I get ready for my annual medical check-up? If this is your first visit to your ...

  16. JAEA Takasaki annual report 2005

    International Nuclear Information System (INIS)

    JAEA Takasaki annual report 2005 describes research and development activities performed from April 1, 2005 to March 31, 2006 with Takasaki Ion Accelerators for Advanced Radiation Application (TIARA, four ion accelerators), and those in a recent few years with electron/gamma-ray irradiation facilities (an electron accelerator and three 60Co gamma-ray irradiation facilities) at Takasaki Advanced Radiation Research Institute, Japan Atomic Energy Agency (JAEA Takasaki). These activities are classified into four research fields: 1) nuclear and energy engineering, 2) environmental conservation and resource security, 3) biotechnology and medical application, 4) advanced materials, analysis and novel technology. This annual report contains 182 reports consisting of 174 research papers and 8 status reports on operation/maintenance of above irradiation facilities, and a list of publications, related press-releases, IV programs, patents, and the type of research collaborations as Appendices. (author)

  17. JAEA Takasaki annual report 2006

    International Nuclear Information System (INIS)

    JAEA Takasaki annual report 2006 describes research and development activities performed from April 1, 2006 to March 31, 2007 with Takasaki Ion Accelerators for Advanced Radiation Application (TIARA, four ion accelerators), and electron/gamma-ray irradiation facilities (an electron accelerator and three 60Co gamma-ray irradiation facilities) at Takasaki Advanced Radiation Research Institute, Japan Atomic Energy Agency (JAEA Takasaki). These activities are classified into four research fields: 1) space, nuclear and energy engineering, 2) environmental conservation and resource security, 3) biotechnology and medical application, and 4) advanced materials, analysis and novel technology. This annual report contains 186 reports consisting of 178 research papers and 8 status reports on operation/maintenance of the irradiation facilities described above, and a list of publications, related press-releases, TV programs, patents, and the type of research collaborations as Appendices. (author)

  18. JAEA Takasaki annual report 2013

    International Nuclear Information System (INIS)

    JAEA Takasaki annual report 2013 describes research and development activities performed from April 1, 2013 to March 31, 2014 mainly with Takasaki Ion Accelerators for Advanced Radiation Application (TIARA, four ion accelerators), and electron/gamma-ray irradiation facilities (an electron accelerator and three 60Co gamma-ray irradiation facilities) at Takasaki Advanced Radiation Research Institute, Japan Atomic Energy Agency (JAEA Takasaki). These activities are classified into four research fields: 1) Space, Nuclear and Energy Engineering, 2) Environmental Conservation and Resource Exploitation, 3) Medical and Biotechnological Application, and 4) Advanced Materials, Analysis and Novel Technology. This annual report contains 169 reports consisting of 160 research papers and 9 status reports on operation/maintenance of the irradiation facilities described above, a list of publications, patents, related press-releases, television broadcasting, and the type of research collaborations as appendices. (author)

  19. JAEA Takasaki annual report 2009

    International Nuclear Information System (INIS)

    JAEA Takasaki annual report 2009 describes research and development activities performed from April 1, 2009 to March 31, 2010 with Takasaki Ion Accelerators for Advanced Radiation Application (TIARA, four ion accelerators), and electron/gamma-ray irradiation facilities (an electron accelerator and three 60Co gamma-ray irradiation facilities) at Takasaki Advanced Radiation Research Institute, Japan Atomic Energy Agency (JAEA Takasaki). These activities are classified into four research fields: 1) space, nuclear and energy engineering, 2) environmental conservation and resource security, 3) biotechnology and medical application, and 4) advanced materials, analysis and novel technology. This annual report contains 165 reports consisting of 157 research papers and 8 status reports on operation/maintenance of the irradiation facilities described above, and a list of publications, patents, related press-releases, TV programs, and the type of research collaborations as Appendices. (author)

  20. JAEA Takasaki annual report 2010

    International Nuclear Information System (INIS)

    JAEA Takasaki annual report 2010 describes research and development activities performed from April 1, 2010 to March 31, 2011 with Takasaki Ion Accelerators for Advanced Radiation Application (TIARA, four ion accelerators), and electron/gamma-ray irradiation facilities (an electron accelerator and three 60Co gamma-ray irradiation facilities) at Takasaki Advanced Radiation Research Institute, Japan Atomic Energy Agency (JAEA Takasaki). These activities are classified into four research fields: 1) Space, Nuclear and Energy Engineering, 2) Environmental Conservation and Resource Exploitation, 3) Medical and Biotechnological Application, and 4) Advanced Materials, Analysis and Novel Technology. This annual report contains 159 reports consisting of 151 research papers and 8 status reports on operation/maintenance of the irradiation facilities described above, and a list of publications, patents, related press-releases, television broadcasting, and the type of research collaborations as Appendices. (author)

  1. JAEA Takasaki annual report 2012

    International Nuclear Information System (INIS)

    JAEA Takasaki annual report 2012 describes research and development activities performed from April 1, 2012 to March 31, 2013 mainly with Takasaki Ion Accelerators for Advanced Radiation Application (TIARA, four ion accelerators), and electron/gamma-ray irradiation facilities (an electron accelerator and three 60Co gamma-ray irradiation facilities) at Takasaki Advanced Radiation Research Institute, Japan Atomic Energy Agency (JAEA Takasaki). These activities are classified into four research fields: 1) Space, Nuclear and Energy Engineering, 2) Environmental Conservation and Resource Exploitation, 3) Medical and Biotechnological Application, and 4) Advanced Materials, Analysis and Novel Technology. This annual report contains 164 reports consisting of 156 research papers and 8 status reports on operation/maintenance of the irradiation facilities described above, a list of publications, patents, related press-releases, television broadcasting, and the type of research collaborations as appendices. (author)

  2. JAEA Takasaki annual report 2011

    International Nuclear Information System (INIS)

    JAEA Takasaki annual report 2011 describes research and development activities performed from April 1, 2011 to March 31, 2012 with Takasaki Ion Accelerators for Advanced Radiation Application (TIARA, four ion accelerators), and electron/gamma-ray irradiation facilities (an electron accelerator and three 60Co gamma-ray irradiation facilities) at Takasaki Advanced Radiation Research Institute, Japan Atomic Energy Agency (JAEA Takasaki). These activities are classified into four research fields: 1) Space, Nuclear and Energy Engineering, 2) Environmental Conservation and Resource Exploitation, 3) Medical and Biotechnological Application, and 4) Advanced Materials, Analysis and Novel Technology for Facilities. This annual report contains 158 reports consisting of 150 research papers and 8 status reports on operation/maintenance of the irradiation facilities described above, and a list of publications, patents, related press-releases, television broadcasting, and the type of research collaborations as appendices. (author)

  3. JAEA Takasaki annual report 2008

    International Nuclear Information System (INIS)

    JAEA Takasaki annual report 2008 describes research and development activities performed from April 1, 2008 to March 31, 2009 with Takasaki Ion Accelerators for Advanced Radiation Application (TIARA, four ion accelerators), and electron/gamma-ray irradiation facilities (an electron accelerator and three 60Co gamma-ray irradiation facilities) at Takasaki Advanced Radiation Research Institute, Japan Atomic Energy Agency (JAEA Takasaki). These activities are classified into four research fields: 1) space, nuclear and energy engineering, 2) environmental conservation and resource security, 3) biotechnology and medical application, and 4) advanced materials, analysis and novel technology. This annual report contains 161 reports consisting of 153 research papers and 8 status reports on operation/maintenance of the irradiation facilities described above, and a list of publications, related press-releases, TV programs, patents, and the type of research collaborations as Appendices. (author)

  4. JAEA Takasaki annual report 2007

    International Nuclear Information System (INIS)

    JAEA Takasaki annual report 2007 describes research and development activities performed from April 1, 2007 to March 31, 2008 with Takasaki Ion Accelerators for Advanced Radiation Application (TIARA, four ion accelerators), and electron/gamma-ray irradiation facilities (an electron accelerator and three 60Co gamma-ray irradiation facilities) at Takasaki Advanced Radiation Research Institute, Japan Atomic Energy Agency (JAEA Takasaki). These activities are classified into four research fields: 1) space, nuclear and energy engineering, 2) environment conservation and resource security, 3) biotechnology and medical application, and 4) advanced materials, analysis and novel technology. This annual report contains 174 reports consisting of 166 research papers and 8 status reports on operation/maintenance of the irradiation facilities described above, and a list of publications, related press-releases, TV programs, patents, and the type of research collaborations as Appendices. (author)

  5. 45 CFR 1801.1 - Annual Truman Scholarship competition.

    Science.gov (United States)

    2010-10-01

    ... 45 Public Welfare 4 2010-10-01 2010-10-01 false Annual Truman Scholarship competition. 1801.1 Section 1801.1 Public Welfare Regulations Relating to Public Welfare (Continued) HARRY S. TRUMAN... competition. Each year, the Harry S. Truman Scholarship Foundation carries out a nationwide competition...

  6. IRSN annual report 2011

    International Nuclear Information System (INIS)

    IRSN, a public authority with industrial and commercial activities, was set up under Article 5 of French Act No. 2001-398 of May 9, 2001, enacted through Order No. 2002-254 of February 22, 2002. This Order was amended on April 7, 2007. The Institute is placed under the joint authority of the Ministries of Defense, the Environment, Industry, Research, and Health. It is the nation's public service expert in nuclear and radiation risks, and its activities cover all the related scientific and technical issues. Its areas of specialization include the environment and radiological emergency response, human radiation protection in both a medical and professional capacity, and in both normal and post-accident situations, the prevention of major accidents, nuclear reactor safety, as well as safety in plants and laboratories, transport and waste treatment, and nuclear defense expertise. IRSN interacts with all parties concerned by these risks (public authorities, in particular nuclear safety and security authorities, local authorities, companies, research organizations, stakeholders' associations, etc.) to contribute to public policy issues relating to nuclear safety, human and environmental protection against ionizing radiation, and the protection of nuclear materials, facilities, and transport against the risk of malicious acts. This document is the 2011 annual report of IRSN. It presents the Organization, Progress and main activities in 2011, Transparency and communications policy, safety and radiation protection culture of IRSN. The main activities in 2011 are presented in details: 1 - Safety: Safety of existing facilities: Monitoring reactors, Monitoring fuel cycle facilities and experimental reactors, Containment and fire, Natural hazards, Fuels, Accidents, About defense; Conducting assessments of future facilities: Future waste repositories, Reactors of the future; 2 - Security and non-proliferation: Protection against malicious act; Activities of the advisory

  7. Annual Report 2000; Rapport annuel 2000

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2000-07-01

    This annual report gives the activities during the year 2000 and information about the General Direction of the Energy and Raw Materials (DGEMP) in France. The highlights of the year are discussed in the main following topics: public utilities, the Erika oil slick, the petroleum products prices increase, the energy efficiency national program, Eole 2005, the nuclear industry management. The french energy accounting, the publications and the organization of the DGEMP are also provided. (A.L.B.)

  8. Petroleum supply annual 1994, Volume 2

    International Nuclear Information System (INIS)

    The Petroleum Supply Annual (PSA) contains information on the supply and disposition of crude oil and petroleum products. The publication reflects data that were collected from the petroleum industry during 1994 through annual and monthly surveys. The PSA is divided into two volumes. This first volume contains four sections: Summary Statistics, Detailed Statistics, Refinery Capacity, and Oxygenate Capacity each with final annual data. The second volume contains final statistics for each month of 1994, and replaces data previously published in the Petroleum Supply Monthly (PSM). The tables in Volumes 1 and 2 are similarly numbered to facilitate comparison between them. Explanatory Notes, located at the end of this publication, present information describing data collection, sources, estimation methodology, data quality control procedures, modifications to reporting requirements and interpretation of tables. Industry terminology and product definitions are listed alphabetically in the Glossary

  9. Petroleum supply annual 1994, Volume 2

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-06-01

    The Petroleum Supply Annual (PSA) contains information on the supply and disposition of crude oil and petroleum products. The publication reflects data that were collected from the petroleum industry during 1994 through annual and monthly surveys. The PSA is divided into two volumes. This first volume contains four sections: Summary Statistics, Detailed Statistics, Refinery Capacity, and Oxygenate Capacity each with final annual data. The second volume contains final statistics for each month of 1994, and replaces data previously published in the Petroleum Supply Monthly (PSM). The tables in Volumes 1 and 2 are similarly numbered to facilitate comparison between them. Explanatory Notes, located at the end of this publication, present information describing data collection, sources, estimation methodology, data quality control procedures, modifications to reporting requirements and interpretation of tables. Industry terminology and product definitions are listed alphabetically in the Glossary.

  10. 76 FR 53329 - Eleventh Coast Guard District Annual Marine Events

    Science.gov (United States)

    2011-08-26

    ... SECURITY Coast Guard 33 CFR Part 100 RIN 1625-AA08 Eleventh Coast Guard District Annual Marine Events... list of marine events occurring annually within the Eleventh Coast Guard District. These amendments... effective September 26, 2011. ADDRESSES: Comments and material received from the public, as well...

  11. California community water systems annual indicators dataset, 1999-2008

    Data.gov (United States)

    California Environmental Health Tracking Program — This data set contains annual measures of arsenic and nitrates in public drinking water supplies. Data are derived from California Office of Drinking Water (ODW)...

  12. Text Reference Panel at ALPS Annual General Meeting

    OpenAIRE

    Wong, Joyce; Flewelling, Debra; WHITE, Nicole

    2011-01-01

    A panel discussion on text reference initiatives at the Simon Fraser University Library, Langara College Library and Douglas College Library. This panel was held at the 2011 ALPS (Academic Librarians in Public Service section of BCLA) Annual General Meeting.

  13. USCIS FY 2009 Annual Report: H-1B Petitions

    Data.gov (United States)

    Department of Homeland Security — The American Competitiveness and Workforce Improvement Act (ACWIA), Public Law 105-277, Division C, imposes annual reporting requirements on U.S. Citizenship and...

  14. Electric power annual 1995. Volume I

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-07-01

    The Electric Power Annual presents a summary of electric power industry statistics at national, regional, and State levels. The objective of the publication is to provide industry decisionmakers, government policymakers, analysts, and the general public with data that may be used in understanding U.S. electricity markets. The Electric Power Annual is prepared by the Coal and Electric Data and Renewables Division; Office of Coal, Nuclear, Electric and Alternate Fuels; Energy Information Administration (EIA); U.S. Department of Energy. In the private sector, the majority of the users of the Electric Power Annual are researchers and analysts and, ultimately, individuals with policy- and decisionmaking responsibilities in electric utility companies. Financial and investment institutions, economic development organizations interested in new power plant construction, special interest groups, lobbyists, electric power associations, and the news media will find data in the Electric Power Annual useful. In the public sector, users include analysts, researchers, statisticians, and other professionals with regulatory, policy, and program responsibilities for Federal, State, and local governments. The Congress and other legislative bodies may also be interested in general trends related to electricity at State and national levels. Much of the data in these reports can be used in analytic studies to evaluate new legislation. Public service commissions and other special government groups share an interest in State-level statistics. These groups can also compare the statistics for their States with those of other jurisdictions.

  15. Electric power annual 1995. Volume I

    International Nuclear Information System (INIS)

    The Electric Power Annual presents a summary of electric power industry statistics at national, regional, and State levels. The objective of the publication is to provide industry decisionmakers, government policymakers, analysts, and the general public with data that may be used in understanding U.S. electricity markets. The Electric Power Annual is prepared by the Coal and Electric Data and Renewables Division; Office of Coal, Nuclear, Electric and Alternate Fuels; Energy Information Administration (EIA); U.S. Department of Energy. In the private sector, the majority of the users of the Electric Power Annual are researchers and analysts and, ultimately, individuals with policy- and decisionmaking responsibilities in electric utility companies. Financial and investment institutions, economic development organizations interested in new power plant construction, special interest groups, lobbyists, electric power associations, and the news media will find data in the Electric Power Annual useful. In the public sector, users include analysts, researchers, statisticians, and other professionals with regulatory, policy, and program responsibilities for Federal, State, and local governments. The Congress and other legislative bodies may also be interested in general trends related to electricity at State and national levels. Much of the data in these reports can be used in analytic studies to evaluate new legislation. Public service commissions and other special government groups share an interest in State-level statistics. These groups can also compare the statistics for their States with those of other jurisdictions

  16. New Mexico Charter Schools Annual Report

    Science.gov (United States)

    New Mexico Public Education Department, 2013

    2013-01-01

    In 2011, the New Mexico legislature passed changes to the Charter School Act that provided more accountability for both charters and authorizers in New Mexico. As part of that law, the Public Education Department (PED) is asked to submit an annual report on the status of charter schools in New Mexico. This is the first report submitted under that…

  17. Annual report for 1979/80

    International Nuclear Information System (INIS)

    This annual report contains extended abstracts of the work done in the named Institute. These concern nuclear physics, solid state physics, molecule physics, high- and intermediate-energy physics, plasma physics, applied optics, data processing, and instrumental and methodical developments. In addition a list of publications, meetings, and theses is given. (HSI)

  18. CEA Annual progress report 1986

    International Nuclear Information System (INIS)

    This annual report presents the general organization of the CEA, the international relations and politics in nuclear field, the activities (military application, nuclear applied research, ANDRA (National Agency for Radioactive Waste Management), nuclear safety and protection, fundamental research, applied research other than nuclear), the industrial group; among topics about men and means, the budget execution of the public establishment of research. In annex, the nuclear power plants around the world and the principal legislative texts related to CEA or atomic energy published in 1986

  19. SKB Annual Report 1996

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-05-01

    This is the annual report of the Swedish Nuclear Fuel and Waste Management Co (SKB). Part 1 of the report contains an overview of the SKB activities in different fields, and part 2 gives a description of the research and development work on nuclear waste disposal performed during 1996. Lectures and publications as well as reports issued during 1996 are listed in part 3, and summaries of the reports are listed in part 4. The task of SKB is to transport, store and dispose of the spent nuclear fuel and radioactive wastes from the nuclear power plants and to perform the research and development and other measures necessary for this work. SKB is the owner of CLAB, the Central Interim Storage Facility for spent fuel, located at Oskarshamn. CLAB was taken into operation in July 1985 and by the end of 1996 about 2500 tons of spent fuel have been received. At Forsmark the Final Repository for Radioactive Operational Waste (SFR) was taken into operation in April 1988. The repository is situated in crystalline rock under the Baltic Sea. SFR has currently a capacity of about 60000 m{sup 3} or waste. At the end of 1996 at total of 21000 m{sup 3} of waste has been deposited. Transportation from the reactor sites to CLAB and SFR is made by a specially designed ship, M/S Sigyn. The total cost for R,D and D during 1996 amounted to 124 MSEK (about 15 MUSD).

  20. SKB Annual Report 1996

    International Nuclear Information System (INIS)

    This is the annual report of the Swedish Nuclear Fuel and Waste Management Co (SKB). Part 1 of the report contains an overview of the SKB activities in different fields, and part 2 gives a description of the research and development work on nuclear waste disposal performed during 1996. Lectures and publications as well as reports issued during 1996 are listed in part 3, and summaries of the reports are listed in part 4. The task of SKB is to transport, store and dispose of the spent nuclear fuel and radioactive wastes from the nuclear power plants and to perform the research and development and other measures necessary for this work. SKB is the owner of CLAB, the Central Interim Storage Facility for spent fuel, located at Oskarshamn. CLAB was taken into operation in July 1985 and by the end of 1996 about 2500 tons of spent fuel have been received. At Forsmark the Final Repository for Radioactive Operational Waste (SFR) was taken into operation in April 1988. The repository is situated in crystalline rock under the Baltic Sea. SFR has currently a capacity of about 60000 m3 or waste. At the end of 1996 at total of 21000 m3 of waste has been deposited. Transportation from the reactor sites to CLAB and SFR is made by a specially designed ship, M/S Sigyn. The total cost for R,D and D during 1996 amounted to 124 MSEK (about 15 MUSD)

  1. 2003 annual report

    International Nuclear Information System (INIS)

    The 2003 issue of the annual report of the French nuclear safety authority (ASR) presents the activities of the ASR in the following domains: 2003 highlights (the project of law relative to the nuclear transparency and safety, the EPR reactor project, the radioprotection priorities, the inspection of radioprotection, the action plan for the monitoring of patients' exposure to ionizing radiations, the 2003 heat wave and the operation of nuclear power plants, the national plan of radioactive wastes management, the behaviour of long living and high level radioactive wastes, and the European 'nuclear package'), nuclear activities, ionizing radiations and health hazards, organisation of nuclear safety control and radioprotection, radioprotection and nuclear facilities regulation, control of nuclear activities, public information and transparency, international relations, radiological emergency situations, radiological and biomedical activities, industrial and research activities, transport of radioactive materials, safety and radioprotection of nuclear power plants, research and other nuclear facilities, fuel cycle centers, safety of the definitive shut-down and dismantling of nuclear facilities, radioactive waste management and cleansing of polluted sites. (J.S.)

  2. NAGRA annual report 2012

    International Nuclear Information System (INIS)

    This report issued by the Swiss National Co-operative for the Disposal of Radioactive Waste NAGRA reviews the co-operative’s activities in the year 2012. In January 2012, the Swiss Federal Office of Energy (SFOE) announced the 20 proposals made by NAGRA for siting areas for nuclear waste repository surface facilities. The discussions within the framework of regional participation focused on the location and layout of the surface facility within the defined planning perimeter. Progress in NAGRA’s work on the safety-based comparison and further narrowing-down of the geological siting regions for the repositories is reported on. NAGRA’s mandate includes its responsibility for preparing the technical and scientific basis for the safe, long-term management of nuclear waste. Progress on the so-called ‘Sectorial Plan’ which addresses the fundamental question of how to safely dispose of all types of radioactive waste is reported on, as is the process of defining locations in Switzerland for one or more deep repositories for nuclear wastes. Public participation in the process is reported on and geochemical considerations involved in designing the repositories are looked at. The work done in the rock laboratories is summarised and international co-operation is noted. The NAGRA ‘Time Ride’ exhibition is also briefly looked at. The report is completed with listings of those involved in NAGRA’s Board of Directors, commissions and auditors along with the annual financial statement for 2012

  3. Public Values

    DEFF Research Database (Denmark)

    Beck Jørgensen, Torben; Rutgers, Mark R.

    2015-01-01

    administration is approached in terms of processes guided or restricted by public values and as public value creating: public management and public policy-making are both concerned with establishing, following and realizing public values. To study public values a broad perspective is needed. The article suggest......This article provides the introduction to a symposium on contemporary public values research. It is argued that the contribution to this symposium represent a Public Values Perspective, distinct from other specific lines of research that also use public value as a core concept. Public...... a research agenda for this encompasing kind of public values research. Finally the contributions to the symposium are introduced....

  4. RTE - annual report 2006

    International Nuclear Information System (INIS)

    RTE is the French electricity transmission system operator responsible for operation, maintenance and development, With a system which is the largest in Europe - 100,000 kilometres of high voltage and extra-high voltage circuits and 46 cross-border interconnections, plus a key location at the geographical hub - RTE is playing a key role in the development of the European electricity market. RTE is a public service company guaranteeing the efficient operation and reliability of the French power system, and providing equitable access to all system users. The company is part of the EDF Group, but has totally independent management and administration, as required by law. In 2005, RTE became a limited liability company with an Executive Board and Supervisory Board; this corporate status guarantees due neutrality in providing service to all players on the electricity market. RTE is the Number 1 electricity transmission system in Europe with 8,300 employees and 4 billion euros turnover. This activity report presents: 1 - Background: The RTE Transmission System, Message from the Chairman of the Executive Board; 2 - A High Performance Public Service Company at the Hub of the European Electricity Market: 2006 overview and Highlights, RTE Governance and Organisation; 3 - A Year with Many Developments: Satisfied Customers on a More Efficient Market, Reliable, Upgraded Industrial Facilities, A Commitment to Environmentally Friendly Public Service, New Foundations for Human Resources Policy, International Business on the Move, A Good Improvement in Financial Performance; 4 - Glossary; 5 - Management Report: Financial and Legal Information (Highlights in 2006, Market Trends, Business and Results for 2006, Capital, Financial Structure, Prospects for 2007, Other Financial and Legal Information), Information on the Environment (Environment Policy - Review, Respect for Natural Environments and Landscapes, Consultation and Consideration, Research, Continuing Improvement, Environmental

  5. Next generation of sequencing techniques guide the new progress of malignant blood diseases: reports in the 55th ASH annual meeting%下一代测序技术引领下的血液系统恶性肿瘤研究新进展:第55届美国血液学会年会报道

    Institute of Scientific and Technical Information of China (English)

    邱林

    2014-01-01

    The biggest highlight in the 55th ASH annual meeting is a series of revolutionary progress in the field of diagnosis,pathogenesis,prediction,prognosis and minimal residue disease (MRD) of malignant blood diseases guiding with next generation of sequencing (NGS) techniques.This article will share the major contents of 7 papers using NGS techniques in the ASH annual meeting with domestic readers here.%第55届美国血液学会(ASH)年会最大亮点是在下一代测序技术(NGS)引领下,血液系统恶性肿瘤诊断、发病机制、疗效评价、预后分析和微小残留病(MRD)监测等领域出现一系列革命性进步.文章仅将第55届Best ASH中应用NGS技术取得重要突破的7篇论文介绍给国内读者.

  6. An overview of the examination of fuel as follow-up to the 1988 November overpower transient in Pickering NGS-A Unit 1

    International Nuclear Information System (INIS)

    A significant number of fuel elements that sustained power ramps during a forty-minute transient in Pickering NGS-A Unit 1 exhibited stress corrosion cracking failures. These were always located at pellet-interface locations at the end of the element that achieved the largest relative increase in power. In addition, a preference for recrystallized alpha (heat affected transition zone) material was observed. The defect threshold was influenced by pellet geometry. The duration of the transient was too short to influence residual mid-pellet sheath strain or grain growth. Circumferential ridges at pellet interfaces were generally enlarged, and existing grain-boundary fission-gas inventories were vented to the free volume. 5 refs., 4 figs., 1 tab

  7. Annual Report 2010-11

    International Nuclear Information System (INIS)

    Annual report of Pakistan Institute of Nuclear Science and Technology (PINSTECH), Islamabad, Pakistan for the year 2010-11 has been compiled which describe a concise but comprehensive description of R and D activities conducted during this period. PINSTECH has contributed considerably in the discipline of chemical, engineering, environmental and physical sciences. It has always been extending technical help and services to Pakistan Atomic Energy Commission (PAEC) as well as other organizations. There are about 90 publications in summary form which gives the main theme of the research activities. At the end of this report different institute activities like courses organized, collaboration with universities, internship, honours /awards and list of publications during the year is also presented. (A.B.)

  8. LLNL NESHAPs 2014 Annual Report

    Energy Technology Data Exchange (ETDEWEB)

    Wilson, K. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Bertoldo, N. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Gallegos, G. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); MacQueen, D. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Wegrecki, A. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

    2015-07-01

    Lawrence Livermore National Security, LLC operates facilities at Lawrence Livermore National Laboratory (LLNL) where radionuclides are handled and stored. These facilities are subject to the U.S. Environmental Protection Agency (EPA) National Emission Standards for Hazardous Air Pollutants (NESHAPs) in Code of Federal Regulations (CFR) Title 40, Part 61, Subpart H, which regulates radionuclide emissions to air from Department of Energy (DOE) facilities. Specifically, NESHAPs limits the emission of radionuclides to the ambient air to levels resulting in an annual effective dose equivalent of 10 mrem (100 μSv) to any member of the public. Using measured and calculated emissions, and building-specific and common parameters, LLNL personnel applied the EPA-approved computer code, CAP88-PC, Version 4.0.1.17, to calculate the dose to the maximally exposed individual member of the public for the Livermore Site and Site 300.

  9. Applicability and Efficiency of NGS in Routine Diagnosis: In-Depth Performance Analysis of a Complete Workflow for CFTR Mutation Analysis.

    Directory of Open Access Journals (Sweden)

    Adrien Pagin

    Full Text Available Actually, about 2000 sequence variations have been documented in the CFTR gene requiring extensive and multi-step genetic testing in the diagnosis of cystic fibrosis and CFTR-related disorders. We present a two phases study, with validation and performance monitoring, of a single experiment methodology based on multiplex PCR and high throughput sequencing that allows detection of all variants, including large rearrangements, affecting the coding regions plus three deep intronic loci.A total of 340 samples, including 257 patients and 83 previously characterized control samples, were sequenced in 17 MiSeq runs and analyzed with two bioinformatic pipelines in routine diagnostic conditions. We obtained 100% coverage for all the target regions in every tested sample.We correctly identified all the 87 known variants in the control samples and successfully confirmed the 62 variants identified among the patients without observing false positive results. Large rearrangements were identified in 18/18 control samples. Only 17 patient samples showed false positive signals (6.6%, 12 of which showed a borderline result for a single amplicon. We also demonstrated the ability of the assay to detect allele specific dropout of amplicons when a sequence variation occurs at a primer binding site thus limiting the risk for false negative results.We described here the first NGS workflow for CFTR routine analysis that demonstrated equivalent diagnostic performances compared to Sanger sequencing and multiplex ligation-dependent probe amplification. This study illustrates the advantages of NGS in term of scalability, workload reduction and cost-effectiveness in combination with an improvement of the overall data quality due to the simultaneous detection of SNVs and large rearrangements.

  10. Annual Statistical Supplement, 2014

    Data.gov (United States)

    Social Security Administration — The Annual Statistical Supplement, 2014 includes the most comprehensive data available on the Social Security and Supplemental Security Income programs. More than...

  11. Annual Statistical Supplement, 2015

    Data.gov (United States)

    Social Security Administration — The Annual Statistical Supplement, 2015 includes the most comprehensive data available on the Social Security and Supplemental Security Income programs. More than...

  12. Annual Statistical Supplement, 2010

    Data.gov (United States)

    Social Security Administration — The Annual Statistical Supplement, 2010 includes the most comprehensive data available on the Social Security and Supplemental Security Income programs. More than...

  13. Annual Statistical Supplement, 2000

    Data.gov (United States)

    Social Security Administration — The Annual Statistical Supplement, 2000 includes the most comprehensive data available on the Social Security and Supplemental Security Income programs. More than...

  14. Annual Statistical Supplement, 2004

    Data.gov (United States)

    Social Security Administration — The Annual Statistical Supplement, 2004 includes the most comprehensive data available on the Social Security and Supplemental Security Income programs. More than...

  15. Annual Statistical Supplement, 2001

    Data.gov (United States)

    Social Security Administration — The Annual Statistical Supplement, 2001 includes the most comprehensive data available on the Social Security and Supplemental Security Income programs. More than...

  16. Annual Statistical Supplement, 2003

    Data.gov (United States)

    Social Security Administration — The Annual Statistical Supplement, 2003 includes the most comprehensive data available on the Social Security and Supplemental Security Income programs. More than...

  17. Annual Statistical Supplement, 2005

    Data.gov (United States)

    Social Security Administration — The Annual Statistical Supplement, 2005 includes the most comprehensive data available on the Social Security and Supplemental Security Income programs. More than...

  18. Annual Statistical Supplement, 2007

    Data.gov (United States)

    Social Security Administration — The Annual Statistical Supplement, 2007 includes the most comprehensive data available on the Social Security and Supplemental Security Income programs. More than...

  19. Annual Statistical Supplement, 2008

    Data.gov (United States)

    Social Security Administration — The Annual Statistical Supplement, 2008 includes the most comprehensive data available on the Social Security and Supplemental Security Income programs. More than...

  20. Annual Statistical Supplement, 2009

    Data.gov (United States)

    Social Security Administration — The Annual Statistical Supplement, 2009 includes the most comprehensive data available on the Social Security and Supplemental Security Income programs. More than...