WorldWideScience

Sample records for annika prna uuenduste

  1. Annika Eberle | NREL

    Science.gov (United States)

    Annika.Eberle@nrel.gov | 303-384-6932 Annika Eberle is a member of the Technology Systems and Sustainability systems Performing sustainability audits of university campuses Primary research interests Sustainability . Zucker. 2007. Environmental Audit of the Claremont Colleges. Claremont, CA: Claremont University

  2. Fabrication of pRNA nanoparticles to deliver therapeutic RNAs and bioactive compounds into tumor cells

    Science.gov (United States)

    Shu, Yi; Shu, Dan; Haque, Farzin; Guo, Peixuan

    2013-01-01

    RNA nanotechnology is a term that refers to the design, fabrication, and utilization of nanoparticles mainly composed of ribonucleic acids via bottom-up self-assembly. The packaging RNA (pRNA) of the bacteriophage phi29 DNA packaging motor has been developed into a nano-delivery platform. This protocol describes the synthesis, assembly, and functionalization of pRNA nanoparticles based on three ‘toolkits’ derived from pRNA structural features: interlocking loops for hand-in-hand interactions, palindrome sequences for foot-to-foot interactions, and an RNA three-way junction for branch-extension. siRNAs, ribozymes, aptamers, chemical ligands, fluorophores, and other functionalities can also be fused to the pRNA prior to the assembly of the nanoparticles, so as to ensure the production of homogeneous nanoparticles and the retention of appropriate folding and function of the incorporated modules. The resulting self-assembled multivalent pRNA nanoparticles are thermodynamically and chemically stable, and they remain intact at ultra-low concentrations. Gene silencing effects are progressively enhanced with increasing number of siRNA in each pRNA nanoparticle. Systemic injection of the pRNA nanoparticles into xenograft-bearing mice has revealed strong binding to tumors without accumulation in vital organs or tissues. The pRNA-based nano-delivery scaffold paves a new way towards nanotechnological application of pRNA-based nanoparticles for disease detection and treatment. The time required for completing one round of this protocol is 3–4 weeks, including in vitro functional assays, or 2–3 months including in vivo studies. PMID:23928498

  3. Annika Sörenstam – a hybrid personal sports brand

    DEFF Research Database (Denmark)

    Cortsen, Kenneth

    2013-01-01

    method inspired by “symbolic interactionist” aspects and focusing on Annika Sörenstam's commercial success with personal sports branding and its interdependence with sports branding at the product and corporate levels. Data collection was conducted in accordance with interpretative research traditions...... and sports brands at the product and corporate levels – often underlining good ROIs for all involved parties if the sports branding process is executed well strategically. This article presents personal sports branding as a hybrid phenomenon, which is dynamic by heart and part of a well‐coordinated process...

  4. RNA Nanoparticles Derived from Three-Way Junction of Phi29 Motor pRNA Are Resistant to I-125 and Cs-131 Radiation

    Science.gov (United States)

    Li, Hui; Rychahou, Piotr G.; Cui, Zheng; Pi, Fengmei; Evers, B. Mark; Shu, Dan

    2015-01-01

    Radiation reagents that specifically target tumors are in high demand for the treatment of cancer. The emerging field of RNA nanotechnology might provide new opportunities for targeted radiation therapy. This study investigates whether chemically modified RNA nanoparticles derived from the packaging RNA (pRNA) three-way junction (3WJ) of phi29 DNA-packaging motor are resistant to potent I-125 and Cs-131 radiation, which is a prerequisite for utilizing these RNA nanoparticles as carriers for targeted radiation therapy. pRNA 3WJ nanoparticles were constructed and characterized, and the stability of these nanoparticles under I-125 and Cs-131 irradiation with clinically relevant doses was examined. RNA nanoparticles derived from the pRNA 3WJ targeted tumors specifically and they were stable under irradiation of I-125 and Cs-131 with clinically relevant doses ranging from 1 to 90 Gy over a significantly long time up to 20 days, while control plasmid DNA was damaged at 20 Gy or higher. PMID:26017686

  5. Ülikoolihõnguline personalikoolitus Eesti Energias / Annika Kaseorg ; küsitlenud Kadri Kiigema

    Index Scriptorium Estoniae

    Kaseorg, Annika

    2007-01-01

    Eesti Energia alustatud uuest projektist "Nädalalõpu Ülikool" räägib Eesti Energia Jaotusvõrgu personali arendamise projektijuht Annika Kaseorg. Avaseminaril tegi ettekande töövõime säilitamisest TLÜ terviseteaduste ja spordi teaduskonna dekaan Kristjan Port

  6. Tegijad meenutavad "52 üllatust ja ideed" / Annika Maior, Ats Parve, Epp Kubu ...[jt.

    Index Scriptorium Estoniae

    2011-01-01

    Kultuuripealinna ootussarjas osalenud räägivad, kuidas idee teostamine kulges. Projektidest "Fantaasiaaed" (Annika Maior), "Camera obscura" (Ats Parve), "Tallinna bussijaam heliseb" (Epp, Kubu, Tõnis Hiiesalu), "Tänavamiimid" (Mihkel Ernits, Kaarel Väli), "Kunstijärjekord" (Flo Kasearu, Aet Ader, Grete Soosalu, Andra Aaloe)

  7. Mg2+ -Dependent High Mechanical Anisotropy of Three-Way-Junction pRNA as Revealed by Single-Molecule Force Spectroscopy.

    Science.gov (United States)

    Sun, Yang; Di, Weishuai; Li, Yiran; Huang, Wenmao; Wang, Xin; Qin, Meng; Wang, Wei; Cao, Yi

    2017-08-01

    Mechanical anisotropy is ubiquitous in biological tissues but is hard to reproduce in synthetic biomaterials. Developing molecular building blocks with anisotropic mechanical response is the key towards engineering anisotropic biomaterials. The three-way-junction (3WJ) pRNA, derived from ϕ29 DNA packaging motor, shows strong mechanical anisotropy upon Mg 2+ binding. In the absence of Mg 2+ , 3WJ-pRNA is mechanically weak without noticeable mechanical anisotropy. In the presence of Mg 2+ , the unfolding forces can differ by more than 4-fold along different pulling directions, ranging from about 47 pN to about 219 pN. Mechanical anisotropy of 3WJ-pRNA stems from pulling direction dependent cooperativity for the rupture of two Mg 2+ binding sites, which is a novel mechanism for the mechanical anisotropy of biomacromolecules. It is anticipated that 3WJ-pRNA can be used as a key element for the construction of biomaterials with controllable mechanical anisotropy. © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

  8. Ramón Reichert and Annika Richterich, eds., Digital Material/ism Vol. 1, Issue 1 – Digital Culture and Society

    OpenAIRE

    Pournara, Lizzy

    2017-01-01

    Ramón Reichert and Annika Richterich, eds., Digital Material/ism Vol. 1, Issue 1 – Digital Culture and Society Bielefeld: [transcript] Verlag, 2015. ISBN 978-3-8376-3153-1. Lizzy Pournara Aristotle University of Thessaloniki The issue Digital Material/ism edited by Ramón Reichert and Annika Richterich inaugurates the first volume of the journal Digital Culture & Society. The significance of this volume’s work lies in its attempt to redress the concept of materiality in digital environments an...

  9. Heinrich Stahlist värske pilguga / Piret Lotman, Kristiina Ross, Aivar Põldvee, Annika Viht ; intervjueerinud Külli Habicht, Külli Prillop

    Index Scriptorium Estoniae

    2014-01-01

    Heinrich Stahli keelelise ja kultuurilise panuse ning tema rolli väärtustamise teemadel vahetasid mõtteid Eesti Rahvusraamatukogu vanemteadur, teose "Heinrich Stahli elu ja looming" autor Piret Lotman, EKI vanemteadur Kristiina Ross, EKI ja TLÜ ajaloo instituudi vanemteadur Aivar Põldvee, TLÜ lektor Annika Viht ning Stahli teoste "Hand- und Hauszbuch" ja "Leyen-Spiegel" sõnastiku koostajad Külli Prillop ja Külli Habicht

  10. Berliini festival asub uuenduste teele / Lauri Kärk

    Index Scriptorium Estoniae

    Kärk, Lauri, 1954-

    2002-01-01

    Berliini 52. filmifestival algab täna sakslase Tom Tykweri mängufilmiga "Taevas". Festivali uue direktori Dieter Kosslicki uuenduseks on saksa filmi endisest tunduvalt suurem osakaal. Esmakordselt on festivali "Panorama Special" kavas eesti mängufilm, milleks on Peeter Simmi "Head käed". Eesti filmid on esil ka festivali suurel filmiturul

  11. Seltskond / Annika Haas

    Index Scriptorium Estoniae

    Haas, Annika

    2007-01-01

    Turundusagentuur PNG (loovjuhiks Jüri Peetson) ühendas tänuõhtu oma klientidele ja koostööpartneritele hispaania laulja Enrique Iglesiase esinemisega 1. dets. Tallinnas Saku Suurhalli restoranis Platoo

  12. Siidii / Annika Koppel

    Index Scriptorium Estoniae

    Koppel, Annika

    2003-01-01

    Heliplaatidest: Millenia Nova "Narcotic Wide Screen Vista", Trygve Seim "Different Rivers", Eesti Keeled ja Jaak Johanson, Riho Sibul "Sabaga Täht", Peeter Vähi, Fedor Vetkalov, Dmitri Tkachenko "Best Regards From Tallinn And Moscow", Brainstorm "A Day Before Tomorrow", Allegri, Pärt, Tormis "Light"

  13. Siidii / Annika Koppel

    Index Scriptorium Estoniae

    Koppel, Annika

    2003-01-01

    Heliplaatidest: "Careful What You Wish For", Sophie Ellis Bextor "Shoot From The Hip", Josh Groban "Closer", Tori Amos "Tales Of Librarian", Enigma "Voyageur", Eesti Filharmoonia Kammerkoor, dirigent Paul Hillier "The Powers Of Heaven"

  14. Seltskond / Annika Haas

    Index Scriptorium Estoniae

    Haas, Annika

    2008-01-01

    Eesti Kunstimuuseumi Kunstisõprade Seltsi eestvõttel tähistati 4. novembril 2008 Kumule Euroopa aasta muuseumi tiitli andmist. Ühel juuresolevatest fotodest president Toomas Hendrik Ilves koos Kumu kunstimuuseumi direktrissi Sirje Helme ja Eesti Kunstimuuseumi Kunstisõprade Seltsi juhatuse esimehe Enn Kunilaga

  15. Seltskond / Annika Haas

    Index Scriptorium Estoniae

    Haas, Annika

    2007-01-01

    21. veebruaril 2007 tähistas Tiigrihüppe SA Tallinnas Olümpia hotelli konverentsikeskuses konverentsiga "Kogu tõde Tiigrihüppest" Tiigrihüppe programmi 10. aastapäeva. Kohal viibis ka programmi üks algatajaid Toomas Hendrik Ilves

  16. Seltskond / Annika Haas

    Index Scriptorium Estoniae

    Haas, Annika

    2009-01-01

    Eesti Posti peakontori hoovis esitleti Indrek Kaingi kujundatud rahvusmustriga tenniseid. Esimesed eksklusiivsed kaks paari MULK-kirjaga papusid kingiti president Toomas Hendrik Ilvesele ning ettevõtte Skype presidendile Josh Silvermanile

  17. Seltskond / Annika Haas

    Index Scriptorium Estoniae

    Haas, Annika

    2008-01-01

    8. aug. Tallinnas kinos Sõprus ja klubis Juuksur tähistati 30 aasta möödumist ansambli Joy Divison sünnist ja Anton Corbijni debüütfilmi "Control" jõudmist Eesti kinodesse (näidati dokumentaalfilmi "Joy Divison")

  18. Seltskond / Annika Haas

    Index Scriptorium Estoniae

    Haas, Annika

    2006-01-01

    Tallinna Ülikoolis toimunud ameerika kultuurantropoloogi Clifford Geertzi mälestusõhtust, kus esinesid rektor Rein Raud, Eesti Humanitaarinstituudi dotsent Lorenzo Cañás Bottos ja kultuuriteooria lektor Marek Tamm

  19. Seltskond / Annika Haas

    Index Scriptorium Estoniae

    Haas, Annika

    2006-01-01

    Inglise rocklaulja Gordon Matthew Sumneri raamatu "Sting. Murtud muusika" esitlusest Tallinnas Viru Keskuse Rahva Raamatu kaupluses ja kontserdist Saku Suurhallis Broken Musicu nimelise tuuri raames 28. juulil

  20. Baltimaade fiktiivne dokumentaalfoto / Annika Toots

    Index Scriptorium Estoniae

    Toots, Annika

    2011-01-01

    Fotokuu näitus "Sündinud samas kohas: kujutis, mälu ja fiktsioon Baltimaades" Tallinna Kunstihoones 20. nov.-ni 2011. Kuraator Vytautas Michelkevičius. Sigrid Viiri, Marge Monko, Krista Möldri (Eesti), Kaspars Podnieksi, Arnis Balčuse (Läti), Tadas Šarūnase, Milda Zabarauskaitė, Robertas Narkuse, Rimas Sakalauskase ja Ugnius Gelduga (Leedu) töödest

  1. Meie esimesed naisfotograafid / Annika Haas

    Index Scriptorium Estoniae

    Haas, Annika, 1974-

    2016-01-01

    Tallinna fotomuuseumi aastanäitus "Varjust välja. Esimesed naisfotograafid Eestis", kuraatorid Betty Ester-Väljaots, Merili Reinpalu, kunstnik Lilian Juhkam. Eesti naisfotograafide Anna Kuke ja Evi Lembergi eluloolisi andmeid, nende looming

  2. Meie esimesed naisfotograafid / Annika Haas

    Index Scriptorium Estoniae

    Haas, Annika, 1974-

    2015-01-01

    Eesti naisfotograafid Hilja Riet ja Lydia Tarem. Fotomuuseumi aastanäitus "Varjust välja. Esimesed naisfotograafid Eestis", kus tutvustatakse Hilja Rieti, Lydia Taremi, Anna Kuke ja Evi Lembergi loomingut. Näituse kuraatorid Betty Ester-Väljaots, Merili Reinpalu

  3. Vaata ja kuula / Annika Koppel

    Index Scriptorium Estoniae

    Koppel, Annika

    2005-01-01

    Heliplaatidest: Jane Birkin "Randez-Vous", "Chill Brazili", "Jazzland Sampler 2005", Mylene Farmer "Avant Que L'Ombre". Norra ansamblist Jaga Jazzist (kontsert 15. juunil Pirita lillepaviljonis, soojendusesinejaks soome kitarrist Jarmo Saari). Jane Birkin esineb Tallinnas 29. juunil

  4. Norma kasumlikkus kahanes / Annika Matson

    Index Scriptorium Estoniae

    Matson, Annika, 1976-

    2005-01-01

    Autodele turvavöösid tootva Norma ärikasum oli kolmandas kvartalis 23,7 miljonit krooni, mis on ligi viiendiku võrra vähem kui eelmisel aastal samal ajal. Diagramm. Vt. samas: Rootsi analüütik ennustas suvel Norma ülevõtmist

  5. Kaasaegne memento mori / Annika Toots

    Index Scriptorium Estoniae

    Toots, Annika

    2015-01-01

    Hispaania fotokunstnikust Tatiana Abellánist, kelle doktorantuuri uurimisteemaks Murcia ülikoolis oli surma kujutamine ja estetiseerimine kunstis. Tema huvist vanade fotode vastu, seeriast "Fuisteis yo"

  6. Salva pistab rinda hiiglastega / Annika Matson

    Index Scriptorium Estoniae

    Matson, Annika, 1976-

    2006-01-01

    Peamiselt eestlastele kuuluv Salva Kindlustus on turul edukas olnud. Diagrammid: Aktsionärid; Majandusnäitajad. Kommenteerib Peeter Teder. Vt. samas: Amber Trust: Salva Kindlustus on meile korralikult dividende maksnud. Diagramm: Fondi Amber Trust investeeringud

  7. Hoiatus Hansa aktsiale / Annika Matson, Romet Kreek

    Index Scriptorium Estoniae

    Matson, Annika, 1976-

    2004-01-01

    Hollandi pank ING andis kiirelt kallinenud Hansapanga aktsiale müügisoovituse. Diagramm. Lisa: Hansapanga aktsia on Tallinna börsi suunanäitaja; ING prognoosid Hansapanga kohta on seni üsna täppi läinud

  8. Hansapanga aktsia vastu ostuhuvi raugenud / Annika Matson

    Index Scriptorium Estoniae

    Matson, Annika, 1976-

    2004-01-01

    Analüütikud soovitavad Hansapanga aktsiat ostmise asemel hoida. Graafikud: Kümnekordne võit; Aktsia hind järgib ING soovitusi. Vt. samas: ING on seni oma prognoosidega Hansapanga aktsia trendi loonud. Raivo Sormunen. Hansapanga aktsia on julgest investeerijast teinud miljonäri;

  9. Looja, armastus ja haigus / Annika Koppel

    Index Scriptorium Estoniae

    Koppel, Annika

    2003-01-01

    Kolm mängufilmi loojatest, keda tabab parandamatu haigus või vaimne häire - Bille Augusti Ulla Isakssoni romaanil põhinev "Laul Martinile" ("En sang för Martin") : Rootsi-Taani 2001, Richard Eyre'i John Bayley raamatul põhinev "Iris" : Suurbritannia-Ameerika Ühendriigid 2001 ja John Maybury kunstnik Francis Baconi üht elulõiku käsitlev "Armastus on saatanast" ("Love Is the Devil") : Suurbritannia-Prantsusmaa-Jaapan 1998. Lisatud B. Augusti, R. Eyre'i ja J. Maybury lühiandmed ja filmograafia lk. 113

  10. Lasteaiakohta pole - mine maale! / Annika Poldre

    Index Scriptorium Estoniae

    Poldre, Annika

    2006-01-01

    Lastekaitse Liidu pöördumisest õiguskantsler Allar Jõksi poole, et saada hinnangut olukorrale, kus omavalitsused ei täida kohustust lastevanemate ees lasteaiakohtade osas. Poliitikute arvamusi. Omavalitsuste kulutustest lasteaedadele. Tabel: Eestlastest sündinud lapsi aastate kaupa; Aastad (lasteasutuste kohta)

  11. Telekomilt oodatakse puhaskasumi kasvu / Annika Matson

    Index Scriptorium Estoniae

    Matson, Annika, 1976-

    2003-01-01

    Ilmunud ka: Delovõje Vedomosti 22. okt. lk. 29. Nii Eesti kui välismaa analüütikud prognoosivad Eesti Telekomile kolmandaks kvartaliks kuni 10%-list puhaskasumi kasvu. Tabel: investeerimispankade prognoosid Eesti Telekomile 2003. aasta III kvartaliks

  12. Inimesed, kes on armunud filmi / Annika Koppel

    Index Scriptorium Estoniae

    Koppel, Annika

    2001-01-01

    UNICA festivali korraldaja Jaak Järvise abiga antud ülevaade Tallinnas 3.-12. augustini toimuvast UNICA 63. ülemaailmsest amatöörfilmide festivalist. Ka nimekiri selle raames toimunud üheminutiliste filmide maailmameistrivõistluste tulemustest, kus ühe kolmanda preemia sai Tõnu Aru ja Eve Esteri "Friendly". Põhiprogrammis võistleb ka Tõnu Aru "Elu on niisugune"

  13. BIG tahab pangaks saada / Annika Matson

    Index Scriptorium Estoniae

    Matson, Annika, 1976-

    2004-01-01

    Ilmunud ka: Delovõje Vedomosti : Finansõ 19. jaan. 2005 lk. 2. Kiirlaenukontorina tegutsev Balti Investeringute Grupp tahab saada pangaks. Kommenteerivad Ain Hanschmidt, Loit Linnupõld, Indrek Neivelt ja Alvar Jõgeva. Vt. samas: Krista Taim. Väikeinvestor kiidab BIG-i soodsat protsenti; Kiirelt saadud laen tänini kukil ja suhted sugulastega sassis

  14. Seltskond : [fotod] / tekst ja fotod Annika Haas

    Index Scriptorium Estoniae

    2008-01-01

    8. dets. esitles Helle Michelson Eesti Lastekirjanduse Keskuses mälestusteraamatut "Lehitsetud leheküljed: Meenutusi elust ja lastekirjandusest" (Tallinn : Tänapäev, 2008) Kohal viibisid lastekirjanikud Ellen Niit, Jaak Urmet jt.

  15. Cannes : seks ja revolutsioon / Annika Koppel

    Index Scriptorium Estoniae

    Koppel, Annika

    2006-01-01

    Cannes'i filmifestival. Nähtud filmidest : Ron Howardi "Da Vinci kood"("The Code Da Vinci"), hiinlase Lou Ye "Suvepalee", inglase Ken Loach'i "Tuul, mis sasib odrapõldu" ("The Wind that Shakes the Barely"), ungarlase Györgi Palfi "Taksidermia"

  16. Holland toetas ja õpetas toetama / Annika Poldre

    Index Scriptorium Estoniae

    Poldre, Annika

    2006-01-01

    Tänavu lõpetab Eestis tegevuse Hollandi Fondide Ühendus CNF (Cooperating Netherlands Foundation for Central and Eastern Europe), mis loodi 15 aastat tagasi Kesk- ja Ida-Euroopa riikide toetuseks. Eestis rahastati 600 projekti. Vt. ka: Hollandi Fondide Ühenduse suurimad Eestis rahastatud projektid

  17. Microsoft ärkab varjusurmast / Annika Matson

    Index Scriptorium Estoniae

    Matson, Annika, 1976-

    2006-01-01

    Ilmunud ka: Delovõje Vedomosti 22. märts lk. 29. Microsofti aktsiale oodatakse kiiret kasvu tänu Office'i uue põlvkonna seitsmele tootele, mis tulevad välja 2006. aasta teises pooles. Diagramm: Aktsia hind. Vt. samas: Microsoft Office'il on tulemas seitse uut tarkvarapaketti

  18. Terrorism, poliitika ja head silmad / Annika Koppel

    Index Scriptorium Estoniae

    Koppel, Annika

    2005-01-01

    PÖFFi filme - "Kohe paradiisi" ("Paradise Now") : režissöör Hany Abu-Assad : Holland - Saksamaa - Prantsusmaa - Palestiina 2005; "Varastatud silmad" ("Otkradnati ochi") : režissöör Radoslav Spassov : Bulgaaria - Türgi 2005; "Valepidi" ("Wrong Side Up") : režissöör Petr Zelenka : Tšehhi 2005

  19. Muutuv kommunikatsioon kultuuri ei ohusta / Annika Poldre

    Index Scriptorium Estoniae

    Poldre, Annika

    2011-01-01

    Tallinna Konverentside poolt Sokos Hotel Virus 28. aprillil 2011 korraldatud kommunikatsiooni aastakonverentsist "Muutuv kommunikatsioon: vana ei taha ja uut ei oska. Kuidas edasi?", kus esinesid Ahto Lobjakas, Priit Põiklik, Piia Tamm ja Marju Lauristin

  20. Kuldne Palmioks liikus Aasiasse / Annika Koppel

    Index Scriptorium Estoniae

    Koppel, Annika

    2010-01-01

    Kokkuvõtteid 63-st Cannes'i filmifestivalist. Kuldse Palmioksa pälvis Apichatpong Weerasethakuli "Onu Boonmee, kes oskab meelde tuletada oma eelmisi elusid", grand prix' sai Xavier Beauvois' "Jumalatest ja inimestest". Nimekiri võitnud töödest

  1. Eesti urbanismi disko Veneetsias / Annika Koppel

    Index Scriptorium Estoniae

    Koppel, Annika

    2000-01-01

    Kunstiajaloolase Anders Härmi ja arhitekt Tarmo Maiste eneseirooniline projekt "Simulacrum City" Veneetsia arhitektuuribiennaalil - 1990. aastate linnakeskkonna areng Eestis. Peapreemia hispaania arhitekt Alberto Campo Baezale. Kommenteerib Anders Härm

  2. Filmikooli tudengid tegid Wiesbadenis ilma / Annika Koppel

    Index Scriptorium Estoniae

    Koppel, Annika

    2007-01-01

    Noore filmirežissööri Rasmus Merivoo bakalaureusetöö - lühimängufilm "Tulnukas ehk Valdise pääsemine 11 osas", võitis Saksamaal Kesk- ja Ida-Euroopa filmifestivalil "goEast" festivali tudengifilmide võistlusprogrammis (hääletas publik) esimese preemia, kolmanda sai Kaupo Kruusiaugu "Meister". Noortele jagus ka teisi auhindu (dokumentaal- ja eksperimentaalfilmide tene preemia Birgit Demidova "Ninanokkija apelsinile", 1500 eurot Joosep Matjuse "Suvisele dokumentaalile") ning koostööfilmi auhinna said Heilika Võsu ja Ülo Pikkov, kes teevad koos sakslastega filmi "Normaalne". Mängufilmide peapreemia Kuldse Lily sai venelase Ivan Võrõpajevi "Eufooria". Eestist osales "Jan Uuspõld läheb Tartusse" ja dokfilmide võistlusprogrammis "Müümise kunst"

  3. Seltskond : [fotod] / tekst ja fotod Annika Haas

    Index Scriptorium Estoniae

    2008-01-01

    29. mail tutvustasid autorid oma lasteraamatuid Eesti Lastekirjanduse Keskuses: Kass, Kristiina. Petra lood. [Tallinn] : Tänapäev, 2008 ; Vainola, Kätlin. Mia, Konrad ja avanevad uksed : [jutustus] / pildid joonistanud Toomas Pääsuke. [Tallinn] : Tänapäev, 2008 ; Saarna, Meelike. Mattias ja mamma. [Tallinn] : Tänapäev, 2008

  4. Maailma naised toetavad Kiikla lastekodu / Annika Poldre

    Index Scriptorium Estoniae

    Poldre, Annika

    2006-01-01

    Eestit külastasid rahvusvahelise naisteorganisatsiooni Soroptimist International president Lynn Dunning Suurbritanniast ja asepresident Kirsten Sveder Rootsist. Külaskäigu eesmärk oli tutvuda Eesti soroptimistide ettevõtmisega - Ida-Virumaale Kiiklasse rajatava noortekoduga. Lisa: Soroptimistide klubid

  5. Wiesbadeni publik hindas Eesti tudengifilmi / Annika Koppel

    Index Scriptorium Estoniae

    Koppel, Annika

    2007-01-01

    Noore filmirežissööri Rasmus Merivoo bakalaureusetöö - lühimängufilm "Tulnukas ehk Valdise pääsemine 11 osas", võitis Saksamaal Kesk- ja Ida-Euroopa filmifestivalil "goEast" festivali tudengifilmide võistlusprogrammis (hääletas publik) esimese preemia, kolmanda sai Kaupo Kruusiaugu "Meister". Noortele jagus ka teisi auhindu (dokumentaal- ja eksperimentaalfilmide tene preemia Birgit Demidova "Ninanokkija apelsinile") ning koostööfilmi auhinna said Heilika Võsu ja Ülo Pikkov, kes teevad koos sakslastega filmi "Normaalne". Mängufilmide peapreemia Kuldse Lily sai venelase Ivan Võrõpajevi "Eufooria". Tänavu kutsuti festivalile Tirana, Tessau ja Kasseli filmikoolide kõrval ka Balti Filmi- ja Meediakool

  6. Kolm noort said majandusteadlase stipendiumi / Annika Poldre

    Index Scriptorium Estoniae

    Poldre, Annika

    2007-01-01

    Sihtasutuse Rein Otsasoni Fond noorte majandusteadlaste stipendiumid said Tallinna tehnikaülikooli majandusteaduskonna doktorandid Jelena Hartshenko ja Aaro Hazak ning Tartu ülikooli majandusteaduskonna magistriõppe üliõpilane Mark Kantshukov

  7. Kaks miljardit jaotub Balti riikides paljude sektorite vahel / Annika Matson

    Index Scriptorium Estoniae

    Matson, Annika, 1976-

    2004-01-01

    Soome investeerimisfondi Danske Capital Finland loodud investeerimisfond Amber Trust II investeerib Balti riikidesse 2 miljardit krooni. Diagramm: Amber Trust S.C.A. fondi osalused Balti riikide ettevõtetes. Lisa: Turg täis, aga fonde tuleb juurde

  8. Viking valitseb Rootsi-Soome liinil / Annika Matson

    Index Scriptorium Estoniae

    Matson, Annika, 1976-

    2004-01-01

    Viking Line'il Soome ja Rootsi vahelisel liinil reisijateveos suurim turuosa. Ettevõtte tegevusest, majandustulemustest. Kommenteerib Romet Kreek. Diagrammid: Soome ja Rootsi vahel sõitvatest laevadest pea iga teine on punane; Tõusuaastaid olnud vaid neli

  9. TeliaSonera tahab Balti riikides enamusosalust / Annika Matson

    Index Scriptorium Estoniae

    Matson, Annika, 1976-

    2003-01-01

    Hollandi investeerimispanga ING analüüsist Eesti Telekom AS-i kohta ja TeliaSonera strateegilisest eesmärgist saada enda valdusesse enamusosalus kõigis Balti riikide telekomiettevõtetes. Diagramm: TeliaSoneral on enamusosalus hetkel vaid Leedu Telekomis

  10. Balti riikide firmadesse voolab veel 2 miljardit krooni / Annika Matson

    Index Scriptorium Estoniae

    Matson, Annika, 1976-

    2004-01-01

    Ilmunud ka: Delovõje Vedomosti 15. dets. lk. 33. Fondist Amber Trust II S.C.A., mis hakkab investeerima Balti riikidesse. Lisa: Firebirdi fondid ja Danske Capital praegu Tallinna börsil ligi 400 mln krooniga. Diagrammid

  11. USA investor loodab raha teenida Brasiilias ja Hiinas / Annika Matson

    Index Scriptorium Estoniae

    Matson, Annika, 1976-

    2006-01-01

    Ilmunud ka: Delovõje Vedomosti 14. juuni lk. 25. USA investeerimisfirma Cumberland Advisors juht David Kotok osales Äripäeva korraldatud majanduskollokviumil. Tema investeerimisfirma on paigutanud raha Ida-Euroopasse, Hiinasse ja Lõuna-Ameerikasse. Vt. samas: Väikeinvestoril tuleb aktsiaid ostes kodutööd teha

  12. The American dramatists' vision of the 1950s / Annika Namme

    Index Scriptorium Estoniae

    Namme, Annika

    2004-01-01

    Ameerika unelmast 1950. aastate ameerika näidendites, Arthur Milleri näidendis "Salemi nõiad" (The Crucible, 1953) ning Edward Albee näidendites "Bessie Smithi surm" (The Death of Bessie Smith, 1959) ja "Ameerika unelm" (The American Dream, 1961)

  13. Hiina kiire majanduskasv kergitab nafta hinda / Lauri Matsulevitsh, Annika Matson

    Index Scriptorium Estoniae

    Matsulevitsh, Lauri

    2005-01-01

    Toornafta hind on praegu rekordiliselt kõrgel tasemel, tõustes 12. augustil Londoni naftabörsil 66,45 dollarini barrelist. Hinnatõusu peamisteks põhjusteks peetakse Pärsia lahe ebastabiilset olukorda ja Aasia kiirest majanduskasvust tingitud tarbimise kasvu. Diagrammid: Nafta hind on aastaga kallinenud poole võrra; Nafta tarbimise järsku kasvu on oodata Aasias. Vt. samas lühiintervjuud Statoil Eesti juht Helle Kirsiga: Bensiini hind jõuab 14 kroonini

  14. Norma juht hämas Venemaa osas / Annika Matson

    Index Scriptorium Estoniae

    Matson, Annika, 1976-

    2005-01-01

    Norma juhatuse esimees Peep Siimon teatas osalise tööaja rakendamise vajadust 551 töötaja puhul ning põhjendab seda keerulise olukorraga Venemaal. Diagramm: Vene autoturg kasvab. Vt. samas: Eesti analüütikud osalise tööaja teadet ei pelga. Kommenteerib Valentin Jurkin

  15. Oodatakse Autolivi ülevõtmispakkumist / Annika Matson, Sirje Niitra

    Index Scriptorium Estoniae

    Matson, Annika, 1976-

    2005-01-01

    Norma omaniku Autolivi osatähtsuse suurenemine viitab, et rootslased teevad varsti Norma kaasaktsionäridele ülevõtmispakkumise. Diagramm: Norma müügis domineerib Autoliv. Kommenteerib Raivo Sormunen

  16. Norma aktsionäridel lootus erakorralisele dividendile / Annika Matson

    Index Scriptorium Estoniae

    Matson, Annika, 1976-

    2004-01-01

    Kolmandas kvartalis paremat kasumlikkust näidanud Norma juhtkond teeb aasta alguses nõukogule ettepaneku maksta erakorralisi dividende. Diagramm: Ettevõtetel olev vaba raha aktsia kohta. Kommenteerib Teet Kolts

  17. Norma ja Eesti Telekomi ootused vastuolulised / Annika Matson

    Index Scriptorium Estoniae

    Matson, Annika, 1976-

    2003-01-01

    16.-17. juulil 2003 avalikustavad Eesti Telekom ja Norma oma teise kvartali tulemused. Kõik majandusanalüütikud ennustavad firmadele korralikku kasumit, kuid ootused kõiguvad 25 protsendi ulatuses. Diagramm: Eesti Telekomilt ootab suurimat kasumit Trigon, Normalt Hansapank

  18. Nokia ampsas tagasi suure osa kaotatud turuosast / Annika Matson

    Index Scriptorium Estoniae

    Matson, Annika, 1976-

    2005-01-01

    Turu-uuringutefirma Gartner andmetel võitis mobiiltelefonide tootja Nokia turuosa tagasi juba kolmandat kvartalit järjest, kuid jääb ikkagi alla 2003. aasta neljandale kvartalile. Diagrammid: Nokia turuosa taas kosumas; Analüütikud soovitavad Nokia aktsiat enim osta

  19. Nokia lõi tulemustega pahviks / Annika Matson

    Index Scriptorium Estoniae

    Matson, Annika, 1976-

    2005-01-01

    Ilmunud ka: Delovõje Vedomosti 2. veebr. lk. 29. Mobiiltelefonide tootja Nokia neljanda kvartali käive ja kasum tõukasid aktsia tõusule ning võideti tagasi ka turuosa. Diagramm: Aasta tagusele tasemele Nokia aktsial veel pikk tee

  20. Nokia juht Ollila ründab pessimiste / Annika Matson

    Index Scriptorium Estoniae

    Matson, Annika, 1976-

    2004-01-01

    Nokia juht Jorma Ollila on tuleva aasta mobiiltelefonide turu kasvu suhtes väga optimistlik. Diagrammid: Mobiiltelefonide turg; Nokia aktsia. Vt. samas: Tõnis Oja. G3 oli tehnoloogiamulli üks peategelasi; Andres Kärssin. Nokia on endiselt eestlaste lemmiktelefon

  1. Ood Eesti puidule = Praise to Estonian wood / Annika Matson

    Index Scriptorium Estoniae

    Matson, Annika, 1976-

    2014-01-01

    Eesti puitmajade ekspordist. Firma Kodumaja ehitab Norrasse Bergeni linna maailma kõrgeima puithoone. Metsamasinate tootjast Metsis, mis valmistab harvestereid ja forvardereid. Saaremaal asuvast puitpaaditootjast Vätta Puit. Karl Annuse Prillitehasest, mis valmistab puidust prilliraame. Metsamaterjali mõõtmise nutirakendusest Timber Diameter

  2. Analüütikud soovitavad Amazonist hoiduda / Annika Matson

    Index Scriptorium Estoniae

    Matson, Annika, 1976-

    2004-01-01

    Hansapank Marketsi, Ühispanga ja LHV maaklerid soovitavad jälgida turu arenguid ja USA online-kaubamaja Amazon.com aktsiatesse lähiajal mitte investeerida. Diagramm: Osta Amazon.com-i aktsiat 40 dollari tasemelt

  3. Cannes'i parimad palad Prantsusmaalt ja Itaaliast / Annika Koppel

    Index Scriptorium Estoniae

    Koppel, Annika

    2008-01-01

    61. Cannes'i filmifestivali tulemustest: Kuldse Palmioksa võitis prantslase Laurent Cantet' mängufilm "Klass" ("Müüride vahel" - "Entre les Murs"). Ka teistest auhinnatud filmidest. Lk. 151 nimekiri auhinnatud filmidest

  4. Europa Cinemas auhindas Sõpruse kino / Katrin Rajasaare ; interv. Annika Koppel

    Index Scriptorium Estoniae

    Rajasaare, Katrin

    2005-01-01

    Euroopa ja Vahemere regiooni kinopidajaid ühendav organisatsioon Europa Cinemas kuulutas Sõpruse kino aasta parimaks noorsooürituste korraldamise kategoorias. Oma tööst räägib Sõpruse juht, kes käis ka 18. nov. Budapestis auhinda vastu võtmas

  5. Aasta pressifotod žürii perspektiivist / Annika Haas

    Index Scriptorium Estoniae

    Haas, Annika, 1974-

    2015-01-01

    Konkursist "Aasta pressifoto", pressifotograafide igapäevatööst. "Aasta pressifoto 2014" peapreemia pälvis Dmitri Kotjuhi "Noored suusatajad", portreefoto kategooria võitis Renee Altrovi "Eesti Vabariigi president", uudisfoto kategooria Margus Ansu "Klassijuhataja tunnid", olemusfoto kategooria Priit Simsoni "Kaotatud sõda"

  6. [Presidendipaar Ingrid ja Arnold Rüütel] / Annika Haas

    Index Scriptorium Estoniae

    Haas, Annika

    2007-01-01

    President Arnold Rüütel ja Ingrid Rüütel Briti suursaadiku Nigel Haywoodi lahkumispeol Tallinna Teaduste Akadeemia peasaalis. Foto allkiri: Presidendipaar Ingrid ja Arnold Rüütel kuulavad Briti suursaadiku Nigel Haywoodi kiidulaulu Eesti kiire arengu aadressil

  7. Cannes'is otsiti lootust ja kirge / Annika Koppel

    Index Scriptorium Estoniae

    Koppel, Annika

    2006-01-01

    Cannes'i filmifestivali võitjad. Kuldse Palmioksa võitis Ken Loachi mängufilm "Tuul, mis sasib odrapõldu" ("The Wind That Shakes the Barley"), Grand Prix' sai prantslase Bruno Dumonti film "Flandria sõdalased" ("Flandres"), parim režissöör oli mehhiklane Alejandro Gonzalez Inarritu filmi eest "Paabel" ("Babel"), parima naisnäitleja auhinna sai grupp näitlejannasid Pedro Almodovari filmist "Tagasitulek" ("Volver") ja meesnäitleja auhinna sai samuti grupp näitlejaid prantsuse filmist "Kuulsuse päevad" ("Indigenes"). Žürii eriauhind läks Andrea Arnoldi filmile "Punane tänav" ("Red Road")

  8. Tarkvarahiid Microsoft kergitas kogu aasta kasumiootust / Annika Matson

    Index Scriptorium Estoniae

    Matson, Annika, 1976-

    2006-01-01

    Tarkvaratootja Microsoft tõstis pärast kvartalikasumi kasvu kogu jooksva majandusaasta kasumiprognoosi. Diagramm. Vt. samas: Euroopa Liit võib monopoliseisuses Microsofti trahvida kahe miljoni euroga päevas; Hiina ja India võivad piraatlusest loobuda 10 aasta pärast

  9. Inarritu "Paabel" - eri keeled, sarnane hing / Annika Koppel

    Index Scriptorium Estoniae

    Koppel, Annika

    2006-01-01

    Mängufilm "Paabel" ("Babel") : stsenarist Guillermo Arriaga : režissöör Alejandro Gonzalez Inarritu, võitis Cannes'is parima režissööri auhinna : peaosades Cate Blanchett, Brad Pitt, Gael Garcia Bernal, Koji Yokusho : Ameerika Ühendriigid 2006

  10. Põltsmaa Felix sepitseb Eesti turul julgeid laienemisplaane / Annika Matson

    Index Scriptorium Estoniae

    Matson, Annika, 1976-

    2005-01-01

    Ettevõtte plaanist laieneda Eesti turul lähiaastatel. Diagrammid: Põltsamaa Felixi kasum on kolm viimast aastat kasvanud; Eestlased eelistavad Felixi ketshupit; Töötajate arv stabiilne. Vt. samas: Romet Kreek. Orkla seostub minule Saku Õlletehase suuromaniku BBHga. Diagramm: Orkla aktsia hind tegi peadpööritava tõusu

  11. The American Dramatist's Vision of the 1950s / Annika Namme

    Index Scriptorium Estoniae

    Namme, Annika

    2004-01-01

    Ameerika näitekirjanike Arthur Milleri ("The Crucible", 1953) ja Edward Albee ("The Death of Bessie Smith" 1959, "The American Dream" 1961) näidendite näitel. Kogumiku koostajad on Neil Murray ja Tony Thorne

  12. Prantsuse aktsiatest ahvatlevad Peugeot, Renault ja Arcelor / Annika Matson

    Index Scriptorium Estoniae

    Matson, Annika, 1976-

    2005-01-01

    Pariisi börsi aktsiatest, kus vaieldamatu soosik on maailma suurim terasetootja Arcelor. Tabel: Prantsuse börsi atraktiivseim aktsia hetkel terasetootja Arcelor. Vt. samas: Eestlaste lemmikud Pariisi börsil olid mullu Alcatel ja Alstom

  13. Q Vara juhtimiskoolitus "Kuidas karastus teras" / Annika Vaikla, Keiu Talve

    Index Scriptorium Estoniae

    Vaikla, Annika

    2007-01-01

    Kinnisvarafirma OÜ Q Vara töötajad pidid läbi viima Paide rahvusvahelise triatloni, mis oli läbiviijatele juhtimiskoolituseks. Küsimusele, kas juhtkonnal tekkis kiusatus sekkuda, vastab Q Vara juhatuse esimees Meelis Šokmann

  14. Berlinalel hõrku kinokunsti maitsmas / Annika Koppel

    Index Scriptorium Estoniae

    Koppel, Annika

    2007-01-01

    57. Berliini filmifestivali auhinnatud filmidest. Peaauhind Kuldkaru - hiina režissööri Wang Quanani film "Tuya abielu" ("Tuya de hun shi"), parima režissööri Hõbekaru - iisraellane Joseph Cedar ("Beafort"), parim meesnäitleja - Julio Chavez (Ariel Rotteri "Teine"), parim naisnäitleja - Nina Hossa (Christian Petzoldi "Yella")

  15. USA kiidetud noise-rock Eestis / Annika Haas

    Index Scriptorium Estoniae

    Haas, Annika

    2008-01-01

    Müra-rock'i viljelevast USA duost Magik Markers (ansambel osaleb režissöör Veiko Õunapuu uue mängufilmi "Püha Tõnu kiusamine" võtetel ja annab kaks kontserti - 13. nov. Tallinnas klubis Tapper ja 15. nov. Tartus klubis Trehv)

  16. Nokia sai vastukäivaid soovitusi / Annika Matson

    Index Scriptorium Estoniae

    Matson, Annika, 1976-

    2005-01-01

    Ajalehe Business andmetel kaalub Cisco Systems Nokia ostmist. Analüütikute hinnangul on uudis spekulatsioon. Diagramm: Spekulatsioon kergitas Nokia aktsiat. Tabel: Esmaspäeval antud soovitused Nokia aktsiale. Vt. samas Cisco Systemsilt oodati kasumi kasvu 16 protsendi võrra

  17. Kuldse Palmioksaga pärjati budistlik lugu / Annika Koppel

    Index Scriptorium Estoniae

    Koppel, Annika

    2010-01-01

    63. Cannes'i filmifestivali peaauhinna võitis Tai režissöör Apichatpong Weerasethakull filmiga "Onu Boonmee, kes võis mäletada oma eelnenud elusid", grand prix' sai Xavier Beauvois' "Jumalatest ja inimestest". Teistest võitjatest

  18. Euroopal on kaks põllumajanduspoliitikat / Annika Poldre

    Index Scriptorium Estoniae

    Poldre, Annika

    2005-01-01

    Euroopa Parlamendi põllumajandus- ja maaelu arengu komiteesse kuuluvad sotsiaaldemokraadid Csaba Sandor Tabajdi Ungarist, Bogdan Golik Poolast ning Luis Manuel Capoulas Santos Portugalist peavad Euroopa Liidu põllumajanduspoliitikat liiga liberaalseks

  19. "EurAsia" parimad palad tulid Euroopast / Annika Koppel

    Index Scriptorium Estoniae

    Koppel, Annika

    2011-01-01

    Rahvusvahelise võistlusprogrammi EurAsia võiduteosest, Sergei Loznitsa mängufilmist „Minu õnn”, (Ukraina - Holland - Saksamaa - Prantsusmaa) ja parima režissööri auhinna pälvinud Chris Krause eestiainelisest filmis "Polli päevikud"(Saksamaa - Austria - Eesti). Artikli lõpus loetletud filmifestivali auhinnasaajad

  20. Eestlased Guildhalli sulatuspotis / Ian Horsbrugh ; interv. Annika Koppel

    Index Scriptorium Estoniae

    Horsbrugh, Ian

    2000-01-01

    London Guildhall School of Music and Drama rektor I. Horsbrugh ja klaveriosakonna juhataja R. O'Hara valisid EMAs toimunud ettemängimisel välja uued Balti riikide stipendiaadid. Eestist pälvis aastase stipendiumi M. Mattisen. Guildhalli koolist ja stipendiumidest

  1. Hiina naise lugu võitis Kuldkaru / Annika Koppel

    Index Scriptorium Estoniae

    Koppel, Annika

    2007-01-01

    57. Berliini filmifestivali peaauhind Kuldkaru - hiina režissööri Wang Quanani film "Tuya abielu" ("Tuya de hun shi"), parima režissööri Hõbekaru - iisraellane Joseph Cedar ("Beafort"), parim meesnäitleja - Julio Chavez (Ariel Rotteri "Teine"), parim naisnäitleja - Nina Hossa (Christian Petzoldi "Yella"), väljapaistev kunstiline saavutus - Robert De Niro lavastajatöö ("Hea karjane")

  2. Tõkestatud "Magnus" võitis kaks auhinda / Annika Koppel

    Index Scriptorium Estoniae

    Koppel, Annika

    2008-01-01

    Kadri Kõusaare film "Magnus" (produtsent Donal Fernandes) võitis Saksamaal "GoEasti" Ida- ja Kesk-Euroopa filmikunsti festivalil peapreemia Kuldse Liilia ja rahvusvahelise filmiajakirjanike ühingu preemia. Ka teistest filmidest : serblase Stefan Arsenijevici "Armastus ja teised kuriteod", ukrainlanna EVa Nejmani "Jõe ääres", venelase Aleksei Popogrebski "Lihtsad asjad"

  3. Kõusaare film teenis Cannes'is pika aplausi / Annika Koppel

    Index Scriptorium Estoniae

    Koppel, Annika

    2007-01-01

    60-ndast Cannes'i filmifestivalil esilinastus 18. mail Kadri Kõusaare mängufilm "Magnus" võistlusprogrammis "Vaatenurk". Filmist ja selle vastuvõtust Cannes'is. Ka Michael Moore'ist ja tema dokumentaalfilmist "Sicko"

  4. Salapärased Iraani naised / Annika Koppel

    Index Scriptorium Estoniae

    Koppel, Annika

    2003-01-01

    Artikli autor kohtus Saksamaal Mannheim-Heidelbergi filmifestivalil iraani režissööri Nasser Refaie ja tema abikasa, näitleja Raya Nasiriga. Režissööri film "Eksam" pälvis ühe žürii auhindadest

  5. Citigroupi soovitus teeb Hansapanga aktsia kuulsaks / Annika Matson

    Index Scriptorium Estoniae

    Matson, Annika, 1976-

    2005-01-01

    Maailma suurim pank Citigroup soovitab osta Hansapanga aktsiaid ning kiidab panga kiiret kasvu regioonis ja esmaklassilist juhtimistiimi. Kommenteerivad: Rain Lõhmus, Jüri Mõis, Heldur Meerits. Vt. samas: Ka Leedu Hansabankas tõi hea uudise; Tõnis Oja. Hansapank Wall Streeti suurtegija huviorbiidis

  6. Mannheimis võitis film kodututest lastest / Annika Koppel

    Index Scriptorium Estoniae

    Koppel, Annika

    2002-01-01

    Mannheimi-Heidelbergi 51. filmifestival Saksamaal, kus peaauhinna võitis prantsuse režissööri Christophe Ruggia "Põrgulised" ("Les Diables") ja FIPRESCI ja R:W: Fassbinderi auhinna 9 norra noore režissööri poliitiline pilakomöödia "Enamik inimesi elab Hiinas" ("Folk flest bor I Kina")

  7. Folkloor muutuvas maailmas / Annika Markson ; juhendaja Mari Raidmets

    Index Scriptorium Estoniae

    Markson, Annika

    2008-01-01

    Artikli esimene osa kirjeldab rahvaluulet ja selle arengut tänapäeval, teine osa rahvatantsu ja line-tantsu arengut Eestis ning kolmandas osa räägitakse grafiti ajaloost ja selle rollist tänapäeva ühiskonnas. Töö lisades on välja toodud intervjuud, küsitluste vastused ning diagrammilised ülevaated küsitlustest

  8. Metsik ida rahulikus läänes / Annika Koppel

    Index Scriptorium Estoniae

    Koppel, Annika

    2011-01-01

    6.-13. aprillini Saksamaal Wiesbadenis toimunud filmifestivalist "goEast", kus näidati filme Ida- ja Kesk-Euroopast. Festivali peaauhinna sai Andrei Balabanovi film "Katlakütja" (Venemaa), parima režissööri auhinna sai Marian Crisan filmiga "Homme"

  9. Eesti film kumab pimedas öös / Annika Koppel

    Index Scriptorium Estoniae

    Koppel, Annika

    1999-01-01

    III Pimedate Ööde Filmifestivalil esilinastuvad Eesti-Läti ühisprojekt "Kolm lugu", kus ka Ervin Õunapuu "Kõrbekuu" ja Peeter Simmi "Aida" ning rahvusvaheline videokassett "Identiteet", kus ka Jaak Kilmi lühifilm "Inimkaamera"

  10. Koos presidendiga kuningal külas / Annika Poldre

    Index Scriptorium Estoniae

    Poldre, Annika

    2007-01-01

    President Toomas Hendrik Ilvese ja proua Evelin Ilvese riigivisiidist Hispaania kuningriiki 8.-11.07.2007. Külalised võtsid vastu kuningas Juan Carlos ja kuninganna Sofia. T.H. Ilves esines kõnega "El futuro de Europa" kuningliku Elcano Instituudi konverentsil, kohtus Madridi ja Barcelona linnapeadega, Hispaania peaministri ning parlamendi alamkoja spiikriga, avas äriseminari, asetas pärja Hispaania langenud sõdurite monumendi juurde. Proua Evelin Ilves külastas kuningliku gobelääni manufaktuuri ja muuseume. Vt. samas: Hispaania Kuningriigi, Euroopa Liidu, Eesti Vabariigi võrdlus arvudes; Riigivisiidil käisid. Presidenti saatnud delegatsiooni koosseis; Reisimulje. Kommenteerivad: ELKE Mööbel OÜ juhatuse esimees Peeter Kangur ja ELKE GROUP ASi nõukogu esimees Vello Väinsalu

  11. Mäss Norma pardal / Annika Matson, Katre Pilvinski

    Index Scriptorium Estoniae

    Matson, Annika, 1976-

    2008-01-01

    Norma väikeaktsionärid nõuavad Norma juhilt Peep Siimonilt suuremat dividendi ja et ettevõte ostaks neilt kuni kümnendiku aktsiaid tagasi turuhinnast ligi kaks korda kõrgema hinnaga. Diagramm: Norma dividendimaksed. Kommenteerib Sander Danil. Vt. ka Enn Tossi art. "Norma tegevus ajab väikeaktsionäri vihale" Äripäev 8. mai lk. 3

  12. Maailm Berlinale filmipeeglis. Kus oleme meie? / Annika Koppel

    Index Scriptorium Estoniae

    Koppel, Annika

    2006-01-01

    56. Berliini filmifestivali auhinnasaajatest- Kuldkaru saaja bosnialanna Jasmila Zhbavic filmi "Grbavica" eest, režii Hõbekaru saajad britid Michael Winterbottom ja Mat Whitecross filmi eest "Teekond Guatanamosse" ("The Road to Guantanamo"), parima filmi Hõbekaru ja debüüdiauhinna saanud taanlanna Pernille Fischer Christensen filmi "Seep" eest ja iraanlane Jafar Panahi filmi "Suluseis" eest. Parimad näitlejad olid Sandra Hüller ja Moritz Bleibtreu. Eestlased filmiturul. Lisatud nimekiri "56. Berliini rahvusvahelise filmifestivali auhinnad"

  13. Kolmekesi ühest Peipsi-äärsest asjast / Tiit Lepp

    Index Scriptorium Estoniae

    Lepp, Tiit

    2007-01-01

    Annika Haasi, Birgit Püve ja Age Petersoni fotonäitus "Prichudie, Revisited" Peipsiääre valla inimestest ja elust-olust kunstikeskuses AmbulARToorium Tartumaaal. Kommenteerinud Annika Haas, Birgit Püve, Age Peterson

  14. The European Union and National Criminal Law

    DEFF Research Database (Denmark)

    Greve, Vagn

    1995-01-01

    Beware of Punishment. Annika Snare (ed.) Beware of Punishment. On the Utility and Futility of Criminal Law......Beware of Punishment. Annika Snare (ed.) Beware of Punishment. On the Utility and Futility of Criminal Law...

  15. Uus Eesti riskifond on rikaste eraisikute pärusmaa / Annika Matson

    Index Scriptorium Estoniae

    Matson, Annika, 1976-

    2004-01-01

    Ilmunud ka: Delovõje Vedomosti 29. sept. lk. 29. LHV toob Eesti turule teise kinnise hedge- ehk riskifondi Global Opportunity. Diagramm: LHV riskifondide indeksid. Lisa: Mis on riskifond?; Järjest rohkem ameeriklasi otsustab riskifondidesse investeerida

  16. Ärimehed ootavad Tallinna börsile tõusu / Annika Matson

    Index Scriptorium Estoniae

    Matson, Annika, 1976-

    2005-01-01

    Eesti ärimeeste ja investeerimisspetsialistide prognoosid Tallinna börsile. Vt. samas: Lauri Matsulevitsh. Spetsialistid on konservatiivsemad. Küsitlusele vastavad ettevõtja Rein Kilk, kunagise börsifirma Pennu Computer Technology asutaja Alo Koop, Audentese ülikooli erakorraline professor Arno Kirt, ettevõtja Jüri Käo, ettevõtja Neinar Seli, investor Rain Lõhmus, Suprema aktsiturgude osakonna juht Sten Sumberg, Trigoni investeerimisjuht Toomas Reisenbuk, Profiti tegevtoimetaja Villu Zirnask. Diagramm: Keskmiselt oodatakse ÄP indeksile 4,4% tõusu

  17. Hinnasõda Soome mobiiliturul kukutas TeliaSonera aktsiat / Annika Matson

    Index Scriptorium Estoniae

    Matson, Annika, 1976-

    2005-01-01

    Telekomifirma TeliaSonera aktsia hind langes 26. apr. Stockholmi börsil 6,4%, mis on kahe viimase aasta suurim langus. Vt. samas: ING soovitas eile Eesti Telekomi müüa; TeliaSonera Soome tegevdirektor lahkub ametist ilma hüvitiseta. Lisa: Välja käidud kehv prognoos langetas aktsiat päevaga 6,4%

  18. Hollandi pank soovitab Eesti Telekomi müüa / Annika Matson

    Index Scriptorium Estoniae

    Matson, Annika, 1976-

    2004-01-01

    Hollandi investeerimispanga ING jt pankade analüütikute soovitused tehinguteks Eesti Telekomi aktsiatega. Graafik: Telekomi aktsia on ING arvates ligi neljandiku võrra ülehinnatud. Vt. samas: ING-l on üle maailma 60 miljonit klienti

  19. "Berlinale 2008" - küll jahe, aga lahe! / Annika Koppel

    Index Scriptorium Estoniae

    Koppel, Annika

    2008-01-01

    Berliini 58. filmifestivali auhinnasaajatest : Kuldkaru võitis Brasiilia produtsendi ja režissööri Jose Padilha mängufilm "Eliitrühm", Hõbekaru USA režissööri Errol Morrise dokfilm "Tavaprotseduur". Parima režissööri Hõbekaru läks Paul Thomas Andersonile mängufilmi "Veri hakkab voolama" eest. Parima näitleja auhinna said Sally Hawkins ja Reza Naji, Alfred Baueri auhinna sai Fernando Eimbcke "Tahoe järv"

  20. Cannes loodab häid üllatusi Ida-Euroopast / Annika Koppel

    Index Scriptorium Estoniae

    Koppel, Annika

    2007-01-01

    Algas 60. Cannes'i filmifestival. Eestit esindav Kadri Kõusaare filmi "Magnus" peaks esilinastuma 18. mail, kui kohtuotsus näitamiskeelu kohta seda ei takista. Pikemalt avafilmist - Wong Kar-Wai "My Blueberry Nights", ka venelase Andrei Zvjagintsevi filmist "Pagendus"

  1. Nutikad eestlased, küsige minult raha / Steve Jürvetson ; intervjueerinud Annika Matson

    Index Scriptorium Estoniae

    Jürvetson, Steve, 1967-

    2009-01-01

    Eesti soost USA riskikapitalist vastab küsimustele, mis puudutavad Eesti aitamist uuele majandustõusule, investeeringuid väärivaid ettevõtteid Eestis, majanduskriisi mõju Jürvetsoni tööle, viimaseid investeeringuid, Skype'i tagasiostuplaane, Skype'i edukust börsil, esimese suure raha teenimist ning start up'ide leidmise viise

  2. Cannes : türgi eksistentsialism ja ameerika kiirtoit / Annika Koppel

    Index Scriptorium Estoniae

    Koppel, Annika

    2006-01-01

    Cannes'i filmifestival. Nähtud filmidest : türklase Nuri Bilge Ceylani "Kliima", soomlase Aki Kaurismäki "Äärelinna tuled", inglase Andrea Arnoldi "Punane tänav" ("Red Road"), ameeriklase Richard Linklateri "Kiirtoidurahvas" ("Fast Food Naton") ja samuti ameeriklase Richard Kelly "Lõuna jutud" ("Southland Tales")

  3. Nafta hinna tõus kukutab taas lennufirmade aktsiaid / Annika Matson

    Index Scriptorium Estoniae

    Matson, Annika, 1976-

    2004-01-01

    USA kaubabörsil rekordilisele tasemele tõusnud nafta hind viis lennukompaniide aktsiad langusse. Prognooside kohaselt võib jätkuv nafta hinnatõus hakata pidurdama maailma majanduskasvu. Diagrammid: Suurima löögi on nafta hinna tõusust saanud USA lennufirmade aktsiad

  4. OPEC usub analüütikutest erinevalt nafta hinna langusesse / Annika Matson

    Index Scriptorium Estoniae

    Matson, Annika, 1976-

    2004-01-01

    Ilmunud ka: Delovõje Vedomosti 1. sept. lk. 19. Vaherahu sõlmimine. Iraagi sõda langetas nafta hinda maailmaturul. Analüütikud prognoosivad aga nafta nõudluse jätkuva kasvu tõttu hinnatõusu. Kommenteerib Pärt Kivaste. Graafik: Toornafta hinna langus veab alla ka naftafirmade aktsiad. Tabel: Viie suurema hulgas kolm Euroopa naftafirmat

  5. Nafta hinna kõikumine solgutab Euroopa lennufirmade aktsiaid / Annika Matson

    Index Scriptorium Estoniae

    Matson, Annika, 1976-

    2004-01-01

    Nafta hinnatõus langetab lennufirmade aktsiate hindasid. Kommenteerib Estonian Air'i asepresident Leela Lilleorg. Graafikud: Euroopa suuremate lennufirmade aktsiad nafta tõmbetuules; Nafta hind rahvusvahelisel naftabörsil Londonis

  6. Maailma suurimad veinitootjad hangivad raha börsilt / Annika Matson

    Index Scriptorium Estoniae

    Matson, Annika, 1976-

    2004-01-01

    Veinitööstustest esimesena börsile läinud Tšiili kontserni Concha y Toro juht Eduardo Guilisasti põhjendab otsust ning analüüsib selle mõju ettevõtte tegevusele. Diagrammid: Suuremate veinitööstuste aktsiad on aasta algusest peamiselt tõusnud. Tabel: Börsil 9 veinitootjat maailma 15st suurimast

  7. Tallinna börsi liikmetel Carnegiel ja Kaupthing Sofil skoor avamata / Annika Matson

    Index Scriptorium Estoniae

    Matson, Annika, 1976-

    2004-01-01

    Neljast Tallinna börsi välismaisest liikmest pole kaks suutnud veel ühelegi investorile Eesti aktsiaid müüa. Diagrammid: Suprema Securities tegi juulis börsil Hansapangast suurema käibe. Lisa: Kaupthing Bank; Garnegie

  8. Varssavi börs saab tänavu enam kui poolsada uustulnukat / Annika Matson

    Index Scriptorium Estoniae

    Matson, Annika, 1976-

    2005-01-01

    Ilmunud ka: Delovõje Vedomosti 16. märts lk. 29. Hollandi panga ING Securities Varssavi analüütik Piotr Palenik on kindel et sealsetesse börsifirmadesse voolab hetkel suurel hulgal välisinvestorite raha ning seetõttu on börsile tulemas rekordarv firmasid. Tabel: Suuremad Poola ettevõtted, mis tulevad tänavu börsile. Vt. samas: Välismaale investeerimise piirang; Eesti investor saab osta kõiki Varssavi börsi aktsiaid

  9. Lapsed taastavad virtuaalselt vanaisade hävitatud maailma / Annika Matson

    Index Scriptorium Estoniae

    Matson, Annika, 1976-

    2000-01-01

    1930. aastail Saksamaal hävitatud sünagoogidest ning Darmstadti tehnikaülikooli arhitektuuriüliõpilaste poolt professor Manfred Koobi juhtimisel 14 sünagoogi virtuaalsest taastamisest. Idee autor oli üliõpilane Marc Grellert

  10. Eesti Statoil seljatas emaettevõtte WiFiga / Annika Matson

    Index Scriptorium Estoniae

    Matson, Annika, 1976-

    2003-01-01

    Eesti teenindusjaamades pakutakse alates suvest tasuta traadita internetiühendust WiFi, Skandinaavimaades on WiFi alles paigutamisel. Lõpuks kasumis: tabelid ja diagrammid. Vt. samas: Statoili arengut pärsib maardlate ammendumine

  11. Nokia aktsia kasvatas eile Eesti investorite vara miljonite võrra / Annika Matson

    Index Scriptorium Estoniae

    Matson, Annika, 1976-

    2004-01-01

    Nokia aktsia hinnatõusu põhjustas teade ettevõtte otsusest tõsta III kvartali käibe- ja kasumiprognoose. Diagrammid: Nokia kasumiprognoos jääb mullusele ikka alla; Nokia aktsia hind tõusis prognooside tõttu

  12. Nokia shokeeris aktsiaturge ootamatu käibehoiatusega / Romet Kreek, Annika Matson

    Index Scriptorium Estoniae

    Kreek, Romet, 1972-

    2004-01-01

    Nokia prognoosis esimeseks kvartaliks varasematest ootustest väiksemat käivet, mis langetas nii Nokia kui ka teiste tehnoloogiafirmade aktsiate hindasid. Diagramm: Käibehoiatus viis eile Nokia aktsia vabalangusse

  13. Nokia võitles tagasi tükikese kaotatud turuosast / Romet Kreek, Annika Matson

    Index Scriptorium Estoniae

    Kreek, Romet, 1972-

    2004-01-01

    Ilmunud ka: Delovõje Vedomosti 3. nov. lk. 33. Mobiiltelefonitootja Nokia võitis kolmandas kvartalis võrreldes teise ja esimese kvartaliga tagasi mobiiltelefonide turuosa. Lisa: Nokia aktsia on saanud osta-soovitusi. Diagramm: Nokia turuosa taas üle 30 protsendi

  14. Nokia müüki pärssis hinnasõda / Annika Matson

    Index Scriptorium Estoniae

    Matson, Annika, 1976-

    2003-01-01

    Nokia mobiiltelefonide müügitulemused võrreldes eelmise aastaga peaaegu ei muutunud. Ettevõtte hinnangul hävitas kasvu Aasia riikide odavate toodete hinnasurve. Diagramm: Nokia käive ja kasum langesid

  15. Õlletootjatest ennustatakse kiireimat kasvu Mehhiko FEMSA-le / Annika Matson

    Index Scriptorium Estoniae

    Matson, Annika, 1976-

    2006-01-01

    Analüütikud soovitavad õlletööstuste aktsiatest enim osta Mehhiko õllevalmistaja FEMSA ja Belgia õlletootja InBev aktsiat. Diagramm: Suurimad õlletootjad. Kommenteerib Tarmo Noop. Vt. samas: Mehhiko rikkaim naine on Coronat tootva Modelo õlletööstuse omanik

  16. Portreefotograafia Oscarid. Kuidas neid küll jagada? / Annika Haas ; kommenteerinud Birgit Püve

    Index Scriptorium Estoniae

    Haas, Annika, 1974-

    2016-01-01

    Londoni rahvusliku portreegalerii (National Portrait Gallery) poolt 2003. aastal algatatud portreefotokonkursist Taylor Wessing Photographic Portrait Prize (TWPPP). 2015. aasta võidutöö: David Stewart "Viis tüdrukut"

  17. Ericsson Euroopas üks suuremaid teadus- ja arendustööle kulutajaid / Annika Matson

    Index Scriptorium Estoniae

    Matson, Annika, 1976-

    2004-01-01

    USA majandusajakiri BusinessWeek koostas kolm edetabelit firmadest, mis kulutavad enim raha teadus- ja arendustööks. Edetabeli kohaselt investeerib Ericsson Euroopa telekommunikatsioonitehnoloogia firmadest enim teadus- ja arendustööks. Diagramm

  18. Oma ninast kaugemal: Aasia ja Euroopa fotokunsti võrdlusi / Annika Haas

    Index Scriptorium Estoniae

    Haas, Annika, 1974-

    2016-01-01

    Rahvusvahelisest portreefotovõistlusest "Kuala Lumpur International Photo Awards 2016" (KLPA 2016). Saksa fotokunstnikest Kathrin Tschirnerist ja Heiko Tiemannist, Jaapani fotokunstnikust Mayumi Suzukist, Jeemeni-Bosnia-Ameerika päritolu fotokunstnikust Alia Alist, Malaisia fotokunstnikest Eiffel Chongist ja Nadirah Zakariyast

  19. Paris Photo 2015 / Kristel Schwede, Annika Haas, Laura Põld, Piret Frey

    Index Scriptorium Estoniae

    2015-01-01

    Rahvusvaheline fotomess "Paris Photo 2015" 12. kuni 15. novembrini Pariisi messihallis Grand Palais. Eestit esindasid messil oma loominguga Jaanus Samma, Sigrid Viir, Krista Mölder (Temnikova & Kasela galerii)

  20. Mehhiko filmikunst toob Cannes'i värskeid tuuli / Annika Koppel

    Index Scriptorium Estoniae

    Koppel, Annika

    2006-01-01

    Cannes'i filmifestival. Nähtud filmidest : soomlase Aki Kaurismäki "Äärelinna tuled" ("Laitakaupungin valot"), hispaanlase Pedro Almodovari "Volver" ("Tagasitulek"), inglase Ken Loach'i "Tuul sasib odrapõldu" ("The Wind that Shakes the Barely"), türklase Nuri Bilge Ceylani "Kliima", itaallase Nanni Moretti "Kaiman" ("The Caiman"), mehhiklase Alejandro Gonzalez Inarritu USA mängufilm Brad Pitt'iga peaosas "Paabel" ("Babel")

  1. Kuldne Palmioks läks filmile Iiri vabadusvõitlusest / Annika Koppel

    Index Scriptorium Estoniae

    Koppel, Annika

    2006-01-01

    Cannes'i filmifestivali võitjad. Kuldse Palmioksa võitis Ken Loachi mängufilm "Tuul, mis sasib odrapõldu" ("The Wind That Shakes the Barley") : stsenarist Paul Laverty, parim režissöör oli mehhiklane Alejandro Gonzalez Inarritu filmi eest "Paabel" ("Babel"). Lisatud nimekiri "Võitjad"

  2. Baltimaade rahvusraamatukogude direktorid kirjutasid alla koostööleppele / Annika Koppel

    Index Scriptorium Estoniae

    Koppel, Annika, 1964-

    2014-01-01

    23. aprillil 2014 kirjutasid Eesti Rahvusraamatukogu peadirektor Janne Andresoo, Läti Rahvusraamatukogu direktor Andris Vilks ja Leedu Rahvusraamatukogu peadirektor Renaldas Gudauskas alla koostööd tihendavale memorandumile. Artiklile on lisatud koostöömemorandumi tekst

  3. IBM Eesti loodab ELiga liitumisest tulu lõigata / Annika Matson

    Index Scriptorium Estoniae

    Matson, Annika, 1976-

    2003-01-01

    Maailma juhtiva infotehnoloogiafirma IBM Eesti tütarettevõte näeb Eesti liitumises Euroopa Liiduga endale uusi võimalusi Euroopa turul, eriti dokumendihalduse valdkonnas. Diagramm: IBMi aktsia viimased kolm aastat

  4. Hansa Ida-Euroopa fond edetabelis teist kuud jutti esimene / Annika Matson

    Index Scriptorium Estoniae

    Matson, Annika, 1976-

    2005-01-01

    Ajakirja The Wall Street Journal Europe Ida-Euroopa ja Venemaa avatud investeerimisfondide edetabelit juhib teist kuud Hansa Ida-Euroopa Aktsiafond. Tabel: Eesti fond juhib The Wall Street Journali edetabelit

  5. Komisjon möönab haigla viga abordi nurjumises / Annika Alasoo, Jüri Saar

    Index Scriptorium Estoniae

    Alasoo, Annika, 1965-

    2000-01-01

    21-aastane meditsiinikooli õpilane lamab neljandat kuud pärast ebaõnnestunud aborti Tallinna Keskhaiglas koomas, sotsiaalministeeriumi arstiabi kvaliteedi komisjon pole oma otsust veel langetanud, kuid komisjoni esimees möönab haiglas tõenäoliselt tehtud vigu

  6. GLEN ehk Eesti noored Aafrikaga tutvust tegemas / Annika Räim

    Index Scriptorium Estoniae

    Räim, Annika

    2008-01-01

    GLEN (Global Education for the Young Europeans) on programm, kus löövad kaasa Euroopa Liidu 12 liikmesmaa mittetulundusühingud ning mis lähetab noori eurooplasi projektide raames kolmeks kuuks arengumaadesse. Artiklis jagavad oma muljeid GLEN-projektis osalenud Kadri Runnel Gruusiast, Kadri Aavik Lõuna-Aafrika Vabariigist, Birgit Rosenberg Sambiast ja Mari Nuga Kamerunist

  7. INES 2000 ja Läänemere ülikool / Annika Tenno

    Index Scriptorium Estoniae

    Tenno, Annika

    2000-01-01

    Läänemere Ülikooli programmiga (The Baltic University Programme) seotud õppejõududel oli võimalus osaleda INES 2000 konverentsil Stockholmis 14.-18. juunil̀2000, peateemaks "Challenges for Science and Engineering in the 21st Century". Tallinna Pedagoogikaülikooli esindajaks oli Tiina Elvisto

  8. Vene riik paneb suure osa nafta hinna tõusust enda taskusse / Annika Matson

    Index Scriptorium Estoniae

    Matson, Annika, 1976-

    2004-01-01

    Tulevikus kahaneb Vene naftagigantide kasum, kuna riik maksustab eksporditava nafta kasumi kuni 90 protsendiga. Vt. samas: Aleksander Malugin. Venemaa metallisektor õitseb. Lisa: Investeerimispankade lemmikud Venemaa börsil

  9. Karm võitlus karmide meeste pärast / Annika Remmel

    Index Scriptorium Estoniae

    Remmel, Annika

    2004-01-01

    Tartu Õlletehase katsetest turule toodud toodete Black ja Disel abil Saku Rocki tarbijaskonnani jõuda. Autor arutleb Rocki edukuse võimalike põhjuste üle. Kommenteerib Siim Nahkur. Diagramm: Saku Rock vs Disel

  10. Uus menüü kasvatas McDonald'si käivet / Annika Matson

    Index Scriptorium Estoniae

    Matson, Annika, 1976-

    2003-01-01

    USA kiirtoiduketi McDonald's vähemalt aasta tegutsenud söögikohtade käive kasvas enam kui 6% eelkõige tänu uuele menüüle ja edukale reklaamikampaaniale. Diagrammid: McDonald'si käive ja kasum kasvasid 13%

  11. CV-Online tahab Londoni börsile / Annika Matson

    Index Scriptorium Estoniae

    Matson, Annika, 1976-

    2004-01-01

    Personaliotsinguga tegelev firma tahab tulevikus minna Londoni ja OMXi börsile. Diagramm: CV-Online Group loodab tänavu esmakordselt positiivse kulumieelse ärikasumi. Vt. samas: Üks CV-Online'i aktsionäridest on ka Google'i aktsionär; Väsinud ootamast esimest IPOt

  12. Islandil peeti filmipidu Kuldse Lunni säras / Annika Koppel

    Index Scriptorium Estoniae

    Koppel, Annika

    2007-01-01

    Muljeid Islandilt, Reykjaviki 4. rahvusvaheliselt filmifestivalilt, mis toimus 27. septembrist 7. oktoobrini s.a. Peauhinna sai ungarlase Csaba Bolloki "Iska reis" ("Iszka utazasa"). Publiku auhinna sai Anton Corbijni "Kontroll", FIPRESCI auhinna sai taanlase Peter Schınau Fogi "Nutmise kunst". Ka iraanlase Fazli Bayrami filmist "Kas sul on veel õuna?" ja argentiinlanna Lucia Puenzo filmist "XXY"

  13. LHV kinnitusel liigutas Lõhmuse nimel miljoneid dollareid Peek / Lauri Matsulevitsh, Annika Matson

    Index Scriptorium Estoniae

    Matsulevitsh, Lauri

    2005-01-01

    Lisaks LHV töötajatele Oliver Peegile ja Kristjan Lepikule on konfidentsiaalse info põhjal toimunud börsitehingutega seotud ka LHV osanik Rain Lõhmus. Vt. samas: Mismoodi Lõhmuse kontol raha liikus?; LHVs kahte tüüpi kontod. Kommenteerivad Joakim Helenius, Jüri Mõis, Rein Kilk ja Indrek Neivelt

  14. Pooljuhtide sektorile oodatakse aasta lõpus kiiret kasvu / Annika Matson

    Index Scriptorium Estoniae

    Matson, Annika, 1976-

    2005-01-01

    Austria suurima majanduslehe Wirtschaftsblatt'i andmetel prognoosivad analüütikud kõikjal maailmas kiibitootjatele järsku käibe tõusu. Diagrammid, tabel: Intel on analüütikute soosik pooljuhtide sektoris

  15. Cannes'i võidufilmi taga on töö noortega / Annika Koppel

    Index Scriptorium Estoniae

    Koppel, Annika

    2008-01-01

    Cannes'i 61. filmifestivali Kuldse Palmioksa võitis prantslase Laurent Cantet' mängufilm "Klass" ("Entre les Murs"). Ka teistest auhinnatud filmidest ja ex-aequo preemia saajatest Clint Eastwoodist ja Catherine Deneuve'ist

  16. Bushi võitu ootavad nafta-, farma- ja kaitsetööstuse firmad / Annika Matson

    Index Scriptorium Estoniae

    Matson, Annika, 1976-

    2004-01-01

    George W. Bushi tagasivalimisest presidendiks võidaksid USA-s naftafirmad, John Kerry võidust aga kinnisvaraettevõtted. Vt. samas: James Denton. Kerry võit langetaks veidi aktsiaturge. Lisa: USA analüütikud eelistavad presidenditoolil Bushi

  17. Tallink on võtnud sihikule Helsingi börsi / Annika Matson

    Index Scriptorium Estoniae

    Matson, Annika, 1976-

    2003-01-01

    Tallink Grupi aktsiakapitali laiendamisel ettevõtte uueks aktsionäriks saanud Citigroup seadis Tallinkile nõude börsilemineku kohta aastaks 2006. Seega plaanib Tallink noteerida oma aktsiad Helsingi börsil. Diagramm: Tallinki käive ja kasum 1997-2003

  18. Ramon Lindali kõrgeim hüpe / Annika Poldre

    Index Scriptorium Estoniae

    Poldre, Annika

    2005-01-01

    Endisele tippsportlasele Ramon Lindalile kuulub viis firmat Eestis ja Soomes, kus valmistatakse eksklusiivsisustust laevadele, hotellidele ja restoranidele ning tegeldakse disaini ja projekteerimisega

  19. Orkaan Katrina kergitas nafta hinna järjekordse rekordini / Annika Matson

    Index Scriptorium Estoniae

    Matson, Annika, 1976-

    2005-01-01

    Naftabarreli hind tõusis börsil üle 70 dollari taseme, kui orkaan Katrina sundis kompaniisid katkestama nafta pumpamise Mehhiko lahel. Diagramm. Vt. samas: Martin Hanson. Orkaan ei sunni aktsiisiplaanist loobuma; Tõnis Arnover. Katrina sundis miljon ameeriklast kodust põgenema

  20. Valitsused on ühes voodis ärimeestega / Annika Matson

    Index Scriptorium Estoniae

    Matson, Annika, 1976-

    2005-01-01

    Ligi 30 aastat tagasi loodud kosmeetika firma The Body Shop asutajast Anita Roddick'ust. Vt. samas: Minu suurim dilemma. Graafik: The Body Shopi aktsia on Londoni börsil näinud nii häid kui ka halbu aegu. Kommenteerib Meelis Milder

  1. Soomlaste Sampo jahvatab eestlaste ja hiinlaste jõul / Annika Koppel

    Index Scriptorium Estoniae

    Koppel, Annika

    2006-01-01

    Soome ja Hiina mütoloogiat ühendav fantaasiafilm "Igavese armastuse sõdalane - Jade Warrior". Film valmis Soome, Hiina, Hollandi ja Eesti ühistöös 2006, režissöör Antti-Jussi Annila. Esilinastus Eestis 20. X kobarkinos

  2. Barbarid, plastiliinijänes, Miriam ja Frank Muller / Annika Koppel

    Index Scriptorium Estoniae

    Koppel, Annika

    2003-01-01

    Nukufilm näitab 4 uut filmi : "Barbarid" (stsenaristid Andrus Kivirähk ja Hardi Volmer, režissöör H. Volmer), "Porgand" (stsenarist Peep Pedmanson, režissöör Pärtel Tall), "Miriami jõulupäkapikk" (stsenaristid Leelo Tungal ja Peep Pedmanson, režissöör Mait Laas), "Kuuvaatleja" (reklaamitreiler Frank Mulleri kellafirmale ; stsenarist, režissöör, kunstnik Mati Kütt)

  3. "Tunnid" tõi Hõbekaru kolmele näitlejannale / Annika Koppel

    Index Scriptorium Estoniae

    Koppel, Annika

    2003-01-01

    Beliini 53. filmifestivali Kuldkaru sai briti režissööri Michael Winterbottomi "Siin maailmas" ("In this World"), parima režii Hõbekaru läks prantslasele Patrice Chereaule filmi "Tema vend" ("Son frere") eest. Lisatud tähtsamate auhindade saajate nimekiri

  4. Innovation in education : the concept of radical innovation adoption / Mary Beth Klinger

    Index Scriptorium Estoniae

    Klinger, Mary Beth

    2004-01-01

    Autor käsitleb organisatsiooni nelja ressursi - organisatsiooniline õppimine, turuorientatsioon, orientatsioon äriprotsessile, tehnoloogiline oportunism - mõju radikaalsete uuenduste ning elektroonilise äri rakendamisele haridusasutustes. Skeem

  5. Vormeliuuendused surus peale Une-Mati / Andrus Allika

    Index Scriptorium Estoniae

    Allika, Andrus

    2003-01-01

    FIA president Max Mosley tunnistas, et mõistis vormelireeglite uuendamise vajadust eelmisel hooajal, kui talle võistluste ajal igavusest uni peale tuli. Lisa: Tosin tähtsamat uuendust 2003. aastal. Tiimide koosseisud

  6. Pärnu rannas ja Pärnumaa rannakülades kõlab muusika / Annika Markson

    Index Scriptorium Estoniae

    Markson, Annika

    2008-01-01

    Kontsertidest 19. juulil: Pärnu Vallikäärus "Väljamõeldud lood" (esinevad ansambel Dagö, Chalice ja Lenna Kuurmaa), Kuursaalis ansambel Kukerpillid ja DJ Riho Pruuli, Beach Clubis üritusel "Jägerett 2008" ansambel Raisk ja DJ Lennart Beat, Sunsetis üritusel "Bacardi Feeling" Dave Benton ja Latin Carribbean Band, Tõstamaa vallas Selistes tantsuõhtu ansambliga Vanad Viisid. 20. juulil: Varbla muuseumi õuel Georg Merioja kitarrikontsert, Pärnu kõlakojas "Pühapäev Valgrega"

  7. Müü dollarid või määra madalaim müügitase / Annika Matson

    Index Scriptorium Estoniae

    Matson, Annika, 1976-

    2003-01-01

    USA dollar nõrgeneb jätkuvalt euro suhtes. Eesti valuutaturgude eksperdid soovitavad eksportööridel dollarid kohe täies mahus või osaliselt ära müüa, kuna kursilanguse peatumist ei ole ette näha. Diagramm: Kuuga on dollar krooni suhtes langenud veel 5,3%

  8. Merko Ehituse aktsialt oodatakse täna tulemuste tõttu tõusu / Annika Matson

    Index Scriptorium Estoniae

    Matson, Annika, 1976-

    2006-01-01

    Ilmunud ka: Delovõje Vedomosti 16. aug. lk. 25. Merko Ehituse teise kvartali kasum kasvas ligi 62% - 135,8 miljoni kroonini. Käive kasvas 41,2% - 1,17 miljardi kroonini. Diagrammid: Merko kasvatas tublisti kasumit. Vt. samas: Väikeaktsionär on lootusrikas. Venek. art. autor ka Dmitri Fokin

  9. Anne Piirsalu juhib kaalujälgijate vägesid Eestis, Soomes ja Lätis / Annika Matson

    Index Scriptorium Estoniae

    Matson, Annika, 1976-

    2005-01-01

    USA-st pärit kaalujälgijate emafirma Weight Watchers aktsiaosalust omab ka Soome, Eesti ja Läti üksuste juht, kuid ei avalda osaluse suurust. Diagrammid: Kaalujälgijad Eestis kolmandat aastat kahjumis; Weight Watchers Internationali aktsia aastaga kallinenud ligi poole võrra. Vt. samas: Peeter Teder. Kaalujälgijad Eestis kahjumis, emafirmal kasvab aga müük ja kasum

  10. Nokia üllatas tulemustega analüütikuid ja saatis aktsia rallile / Annika Matson, Lauri Matsulevitsh

    Index Scriptorium Estoniae

    Matson, Annika, 1976-

    2005-01-01

    Mobiiltelefonitootja Nokia majandustulemustest esimeses kvartalis ning teise kvartali prognoosidest. Diagramm: Tulemuste peale võttis Nokia taas suuna üles. Vt. samas: Rein Küttim on Nokiaga kaubelnud pikemat aega

  11. Rootsi põllumajandusminister Ann-Christin Nykvist : Oleme ühel meelel / Ann-Christin Nykvist ; interv. Annika Poldre

    Index Scriptorium Estoniae

    Nykvist, Ann-Christin

    2004-01-01

    Eestis külaskäigul viibinud Rootsi põllumajandus-, toidu- ja tarbimisminister tutvustab oma tööd, Rootsi põllumajanduspoliitikat, räägib Rootsi toidukaupade eelistamisest, EL-i laienemise mõjust Rootsi põllumajandusele, Rootsi ja Eesti koostööst põllumajanduses

  12. Õhk on tiine aastatuhande vahetuse hüsteeriast / Siim-Tanel Annus ; interv. Annika Koppel

    Index Scriptorium Estoniae

    Annus, Siim-Tanel, 1960-

    1998-01-01

    Kunstnik avas 18. dets. Tallinna Kunstihoones maalinäituse (akrüülmaal koos dolomiidipuru ja liivaga) koos saksa kunstniku Abraham David Christiani pronksskulptuuride ja joonistusteseeriaga "Mississipi"

  13. Miks sponsoreerivad ettevõtted suurüritusi? / Frank Svenhard, Liivi Tadolder ; interv. Annika Rõõmus

    Index Scriptorium Estoniae

    Svenhard, Frank

    2006-01-01

    ALD Automotive Baltikumi tegevdirektor ja AS-i Liviko turundusjuht valikutest sponsoreerimisel, koostööst sponsoreeritavate organisatsioonidega, rahvusvahelise juhtimiskonverentsi EC2006 toetamisest

  14. Seltskond : Tallinna Linnateatri 42. hooaja avasündmus ja kohtumine teatrilegendi Adolf Šapiroga / Annika Haas

    Index Scriptorium Estoniae

    Haas, Annika

    2006-01-01

    5. sept. toimus Tallinna Linnateatri 42. hooaja avamine, sai ülevaate teatri plaanidest ja toimus kohtumine Adolf Šapiroga. Uue hooaja plaanidesse kuulub ka töölepingu sõlmimine Tallinna Ülikooliga

  15. Does timbre affect pitch?: Estimations by musicians and non-musicians / Allan Vurma, Marju Raju, Annika Kuuda

    Index Scriptorium Estoniae

    Vurma, Allan, 1955-

    2011-01-01

    Muusikutel ja mittemuusikutel lasti kuulata lindilt inimhääle, vioola ja trompetihelisid ning hinnata järgmisena kõlanud helikatket võrreldes eelmisega kas "madalaks", "kõrgeks" või "hääles". Leiti, et tämbrist mõjutatud helikõrguse muutused võivad tekitada konflikti subjektiivse tunnetamise ja fundamentaalsel võnkesagedusel põhineva heli hindamise vahel

  16. Teateid sugudevahelisest sõjast ja homorindelt. XIX Pärnu rahvusvaheline dokumentaal- ja antropoloogiafilmide festival / Annika Koppel

    Index Scriptorium Estoniae

    Koppel, Annika

    2005-01-01

    Pärnu 19. dokumentaal- ja antropoloogiafilmide festivalist. Grand prix võitis režissöör Pirjo Honkasalo filmiga "Melanhoolia 3 tuba". Televaatajad valisid oma lemmikuks taani režissööri Lars Westmani filmi "Kas sa armastad mind?". Teistest filmidest : Murray Nosseli "Isainstinkt", Petr Lomi "Pruudirööv kirgiisi moodi", Ellen-Astri Lundby "Neljas naine ja tema Aafrika mees" jt. Lisatud auhinnasaajate nimekiri lk. 144

  17. Viisnurk saab üksuste müügist heal juhul 170 miljonit krooni / Annika Matson

    Index Scriptorium Estoniae

    Matson, Annika, 1976-

    2004-01-01

    AS Viisnurk müüb ehitus- ja spordikaupade divisjonid, liimpuiduvabriku ning vabrikualuse maa. Viisnurga endine tegevjuht Meelis Kukk ning konkureerivate ettevõtete esindajad prognoosivad müügist saadavat tulu. Diagramm: Suurimat tulu loodetakse kasumis ehitusmaterjalide tootmise müügist. Vt. samas: Adrenaliini armastav investor võiks Viisnurga aktsiat osta

  18. Uudised : Lassmann Konservatooriumide Assotsiatsiooni juhatuses. Algab "Con brio 2000". Erkki-Sven Tüüri autorikontsert / Annika Koppel

    Index Scriptorium Estoniae

    Koppel, Annika

    2000-01-01

    P. Lassmann valiti Euroopa Konservatooriumide Assotsiatsiooni juhatuse liikmeks. Eesti noorte interpreetide konkurssfestivalist "Con brio 2000". Tallinna metodisti kirikus toimub 24. nov. E.-S. Tüüri autorikontsert

  19. Oksjon - põnevaim viis kunsti ostmiseks : rahakad kunstiostjad hindavad klassikalisi lillepilte, maastikke ja linnavaateid / Annika Koppel

    Index Scriptorium Estoniae

    Koppel, Annika

    1999-01-01

    Riose galerii korraldatud kunstioksjonist Eesti Rahvusraamatukogus. Pakuti eesti kunstnike töid, ekspert Georg Poslawski. Kunstioksjonitest Inglismaal ja Eestis. Kunstiteadlase Mai Levini kommentaar. Oksjoni oskussõnu. Kallimad tööd

  20. Järvakandi Klaasi edu tõstab emafirma aktsiat New Yorgi börsil / Annika Matson

    Index Scriptorium Estoniae

    Matson, Annika, 1976-

    2005-01-01

    USA kontserni Owens-Illinoisi tütarfirmadest, turuosast, Eesti tütarfirma AS-i Järvakandi Klaas laienemisplaanidest. Diagrammid: Järvakandi Klaas ootab tänavu taas kasumi kasvu; Owens-Illinoisi aktsia on investoritele tulus olnud. Vt. samas: Owens-Illinois on olnud hea aktsia

  1. Oscari-kandidaate jälgiv Berlinale on jõudmas lõpusirgele / Annika Koppel

    Index Scriptorium Estoniae

    Koppel, Annika

    2003-01-01

    Berliini 53. filmifestivali võistlusprogrammis linastunud filmidest - "Tema vend" ("Son frere") : režissöör Patrice Chereau : Prantsusmaa 2003, "Kauged tuled" : režissöör Hans-Christian Schmid : Saksamaa - Poola 2002 ja "Jah, õde! Ei, õde!" : režissöör Pieter Kramers : Holland 2002

  2. Tsharterlennud võivad tulevikus Eestist kaduda / Andres Eilart

    Index Scriptorium Estoniae

    Eilart, Andres

    2002-01-01

    Turismifirmade liidu asepresident Daisy Järva, Tallinna Lennujaama turundusmees Sven Ratassepp ja Horizon Traveli turundusjuht Annika Sillaots tsharterlennundusest Baltikumis. Tabel: kaheksa sihtkohta, kuhu Estonian Air regulaarlende korraldab

  3. Role of the CCA bulge of prohead RNA of bacteriophage ø29 in DNA packaging.

    Science.gov (United States)

    Zhao, Wei; Morais, Marc C; Anderson, Dwight L; Jardine, Paul J; Grimes, Shelley

    2008-11-14

    The oligomeric ring of prohead RNA (pRNA) is an essential component of the ATP-driven DNA packaging motor of bacteriophage ø29. The A-helix of pRNA binds the DNA translocating ATPase gp16 (gene product 16) and the CCA bulge in this helix is essential for DNA packaging in vitro. Mutation of the bulge by base substitution or deletion showed that the size of the bulge, rather than its sequence, is primary in DNA packaging activity. Proheads reconstituted with CCA bulge mutant pRNAs bound the packaging ATPase gp16 and the packaging substrate DNA-gp3, although DNA translocation was not detected with several mutants. Prohead/bulge-mutant pRNA complexes with low packaging activity had a higher rate of ATP hydrolysis per base pair of DNA packaged than proheads with wild-type pRNA. Cryoelectron microscopy three-dimensional reconstruction of proheads reconstituted with a CCA deletion pRNA showed that the protruding pRNA spokes of the motor occupy a different position relative to the head when compared to particles with wild-type pRNA. Therefore, the CCA bulge seems to dictate the orientation of the pRNA spokes. The conformational changes observed for this mutant pRNA may affect gp16 conformation and/or subsequent ATPase-DNA interaction and, consequently, explain the decreased packaging activity observed for CCA mutants.

  4. Structure and assembly of the essential RNA ring component of a viral DNA packaging motor.

    Science.gov (United States)

    Ding, Fang; Lu, Changrui; Zhao, Wei; Rajashankar, Kanagalaghatta R; Anderson, Dwight L; Jardine, Paul J; Grimes, Shelley; Ke, Ailong

    2011-05-03

    Prohead RNA (pRNA) is an essential component in the assembly and operation of the powerful bacteriophage 29 DNA packaging motor. The pRNA forms a multimeric ring via intermolecular base-pairing interactions between protomers that serves to guide the assembly of the ring ATPase that drives DNA packaging. Here we report the quaternary structure of this rare multimeric RNA at 3.5 Å resolution, crystallized as tetrameric rings. Strong quaternary interactions and the inherent flexibility helped rationalize how free pRNA is able to adopt multiple oligomerization states in solution. These characteristics also allowed excellent fitting of the crystallographic pRNA protomers into previous prohead/pRNA cryo-EM reconstructions, supporting the presence of a pentameric, but not hexameric, pRNA ring in the context of the DNA packaging motor. The pentameric pRNA ring anchors itself directly to the phage prohead by interacting specifically with the fivefold symmetric capsid structures that surround the head-tail connector portal. From these contacts, five RNA superhelices project from the pRNA ring, where they serve as scaffolds for binding and assembly of the ring ATPase, and possibly mediate communication between motor components. Construction of structure-based designer pRNAs with little sequence similarity to the wild-type pRNA were shown to fully support the packaging of 29 DNA.

  5. Mis juhtus Puhja kiriklas? : ühe vana tõlke algust otsides / Annika Kilgi, Inge Käsi, Aivar Põldvee ... [jt.

    Index Scriptorium Estoniae

    2014-01-01

    Otsitakse vastust küsimusele, kumb keel oli isa-poja Andreas ja Adrian Virginiuse tõlgitud Vana Testamendi sihtkeel, kas lõunaeesti või põhjaeesti keel ning kas Puhjas toimus midagi tõlketalgute taolist. Artikkel fikseerib uurimise hetkeseisu

  6. Pronkssõduri teisaldamise ainsaks eelduseks on referendum / Kalev Rebane, Roman Zharov, Aleksandr Korobov...[jt.] ; interv. Peeter Ernits, Jüri Aarma, Annika Poldre

    Index Scriptorium Estoniae

    2006-01-01

    Oma seisukohtadest Tallinnas Tõnismäel asuva Teise maailmasõja mälestusmärgi suhtes räägivad isamaaliitlane Kalev Rebane ja vene noorteühenduse Notshnoi Dozor juhid Roman Zharov, Aleksandr Korobov, Oleg Filippov ja Igor Rogo

  7. Orkaan Rita ähvardab rüüstata USA naftatööstuse südame / Annika Matson

    Index Scriptorium Estoniae

    Matson, Annika, 1976-

    2005-01-01

    Orkaan Rita läheneb USA naftatööstuse südamele Texasele, kus toodetakse veerand USA naftatoodetest. Diagramm: Naftafirmade aktsiate tõus. Vt. samas: Bensiini hinna olulist tõusu Eestis prognoosida oleks ennatlik

  8. Tallink jättis eilsel börsipäeval väikeaktsionärid infosulgu / Annika Matson

    Index Scriptorium Estoniae

    Matson, Annika, 1976-

    2006-01-01

    Tallink ei andnud erinevalt Viking Line'ist oma väikeaktsionäridele teada, kas osaleb Silja Line'i ostupakkumisel. Kommenteerib Raul Malmstein. Vt. samas: Lauri Matsulevitsh. Citigroup soovitab Tallinki aktsiat osta

  9. Peetri maja kant Kadriorus saab peagi uue näo / Ulvar Käärt

    Index Scriptorium Estoniae

    Käärt, Ulvar, 1982-

    2009-01-01

    Kadrioru pargis asuva Peeter I maja ja presidendi kantselei vahelise pargiala uuendustöödest. Vt. samas: Presidendi lossi esine suletakse. Peetri aia rekonstrueerimistööde käigus on plaanis pargi territooriumile üle viia ka praegu presidendi kantselei esist paraadplatsi läbiv kõnnitee, kuna kantseleil on plaanis hoone esine ümbritseda piirdeaiaga

  10. Kooli innovatsiooni mudelid maakoolide näitel (KIMA) / Anita Kärner

    Index Scriptorium Estoniae

    Kärner, Anita, 1956-

    2014-01-01

    Ülevaade Eduko analüüsigrandi toetusel nelja Lõuna-Eesti maakonna kooli baasil Tartu Ülikoolis läbi viidud uuringust, mille eesmärgiks oli selgitada, millised on kooli organisatsiooni sisemised ja väliskeskkonna tegurid uuenduste algatamisel ja elluviimisel, millised on koolis muutusi soodustavad ja takistavad tegurid

  11. Ajaloolise ja isikliku mälu arheoloogiast tulevikuvisioonideni / Katrin Kivimaa

    Index Scriptorium Estoniae

    Kivimaa, Katrin, 1969-

    2005-01-01

    Kuni 15. I 2006 Victoria ja Alberti muuseumis avatud näitusest "Mineviku ja tuleviku vahel", mis annab ülevaate hiina kaasaegse foto- ja videokunsti ning dokumentatsioonide kaudu ka performance'i hiljutisest arengust ja hetkeseisust ning toob nähtavale praegust hiina kultuuri mõjutavate traditsioonide ja uuenduste keerulise sasipuntra

  12. Inseneriharidus: vaja on liikuda kõrgema kvaliteedi ja uuenduslikkuse suunas / Hants Kipper

    Index Scriptorium Estoniae

    Kipper, Hants

    2015-01-01

    17.-20. märtsini toimus Tallinna tehnikaülikoolis rahvusvahelise inseneride ühingu IEEE (Institute of Electrical and Electronics Engineers) ülemaailmne insenerihariduse arengule pühendatud aastakonverents EDUCON2015, teemaks „Inseneriharidus tipptaseme ja uuenduste suunas” („Engineering Education towards Excellence and Innovation”)

  13. TP Organics - maheuuringute tehnoloogiaplatvorm

    Index Scriptorium Estoniae

    2015-01-01

    TP Organics on 2008. a loodud Euroopa mahepõllumajanduse ja mahetoidu ning vähese sisendiga põllumajanduse tehnoloogiaplatvorm. Platvormi eesmärk on selgitada välja teadustöö ja uuenduste vajadused ning edastada see info poliitikutele

  14. Kellele oleksid Sina presidendina autasu andnud?

    Index Scriptorium Estoniae

    2011-01-01

    Küsimusele vastavad Vabadusvõitluse muuseumi juhataja Johannes Tõrs, Viimsi Teataja peatoimetaja Annika Poldre, Saue linna elanik Evelin Povel-Puusepp, Muraste elanik Vello Malken, Kuusalu valla elanik Sirje Potisepp ja Kanama postijaama elanik Priit Pillov

  15. Enamik Mõtteloo Sihtkapitali rahast kasvab USA aktsiates / Mart Trummal ; interv. Anne Oja

    Index Scriptorium Estoniae

    Trummal, Mart

    2006-01-01

    Intervjuu Eesti Mõtteloo Sihtkapitali juhi Mart Trummaliga. Tabel: Mõtteloo Sihtkapitali vara on USA-s noteeritud indeksifondides; Investeeringud Eesti aktsiatesse. Diagramm: Finantsinvesteeringute maht. Vt. samas: Anne Oja. Dividendisaajate esirinnas. Kommenteerib Annika Matson

  16. Panustamine töötaja teadmiste testimisse tasub ära / Maio Rannamägi, Kreet Aun, Mari Krumm

    Index Scriptorium Estoniae

    Rannamägi, Maio

    2006-01-01

    If Eesti Kindlustus lõi töötajate teadmiste testimise süsteemi, et omada parimate kindlustusalaste teadmistega personali Eestis. Vt. samas: Teste täiustati pidevalt. Kommenteerivad Heli Kauber ja Annika Jung

  17. Armastus esimesest pilgust / Ene Vool

    Index Scriptorium Estoniae

    Vool, Ene, 1948-

    2005-01-01

    Annika Tederi jaapani stiilis aiast kui tähtsast osast eluruumis, mille kujundamisel on abiks olnud Liina Rehepapp. Jaapani aedadest, jaapanlaste suhtumisest aiakunsti ja lisaks artikkel "Eriilmelised ja -otstarbelised jaapani aiad"

  18. Annad Miksikesele sõrme, võtab terve käe / Sirje Tohver

    Index Scriptorium Estoniae

    Tohver, Sirje, 1948-

    2005-01-01

    Miksikese õppekeskkonnast ja online-kontrolltöödest erinevate Eesti koolide näitel. Kogemusi jagavad õpetajad: Age Tamm, Eha Kaukvere, Tiia Salm, Kaja Kiis, Mare Kütt, Lemme Sulaoja, Annika Lõhmus

  19. Surnukuurikunst elab Tartu anatoomikumis / Aigi Viira

    Index Scriptorium Estoniae

    Viira, Aigi

    1999-01-01

    Vene kunstniku Aleksander Potolitsyni performance'iga avati Tartus kunstiprojekt "Tartu Anatoomikum" ئ 14 euroopa kunstniku (loetletud) installatsioonid. Projekti kuraator on Paul Rodgers. Eestit esindavad Jaan Jaanisoo, Jüri Ojaver, Annika Tonts

  20. Modelliagentuurid toodavad Carmen Kassile tasemel järglasi / Kerli Nõu

    Index Scriptorium Estoniae

    Nõu, Kerli

    2006-01-01

    Eestis tegutsevatest modelliagentuuridest Beatrice Mass Modelnet, Baltic Models OÜ ja Eesti Modelliagentuuri OÜ. Kommentaarid: Tasane algus kulisside taga, Edukad Eesti modellid (Annika Vohta), Eesti uus Carmen Kass (Claudia Meriküla)

  1. 6.-15. X on EKA galeriis...

    Index Scriptorium Estoniae

    2003-01-01

    Keraamika eriala magistrantide näitus "Argilla neljale". Eksponeeritakse Reeli Haameri, Kadri Kivi, Annika Vilippuse ja Matthew Graziano magistriõppe meistriklassi raames valminud töid koos tehnikaid tutvustavate tekstidega

  2. Evolution of Both Host Nation Police Advisory Missions and the Support Provided by the Department of Defense

    Science.gov (United States)

    2012-05-17

    American forces to train throughout the nation, often in partnership with the Panamanian Defense Forces (PDF), until the rise in Manuel Noriega’s power and...Peace and Stability Operations". Annika Hansen, From Congo to Kosovo: Civilian Police in Peace Operations (New York, NY: Oxford University Press...Soldier Support for Operation Uphold Democracy." Armed Forces & Society 23, no. 1 (Fall 1996): 81-96. Hansen, Annika. From Congo to Kosovo: Civilian

  3. Global Structure of a Three-Way Junction in a Phi29 Packaging RNA Dimer Determined Using Site-Directed Spin Labeling

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, Xiaojun; Tung, Chang-Shung; Sowa, Glenna; Hatmal, Ma' mon M.; Haworth, Ian S.; Qin, Peter Z.

    2012-02-08

    The condensation of bacteriophage phi29 genomic DNA into its preformed procapsid requires the DNA packaging motor, which is the strongest known biological motor. The packaging motor is an intricate ring-shaped protein/RNA complex, and its function requires an RNA component called packaging RNA (pRNA). Current structural information on pRNA is limited, which hinders studies of motor function. Here, we used site-directed spin labeling to map the conformation of a pRNA three-way junction that bridges binding sites for the motor ATPase and the procapsid. The studies were carried out on a pRNA dimer, which is the simplest ring-shaped pRNA complex and serves as a functional intermediate during motor assembly. Using a nucleotide-independent labeling scheme, stable nitroxide radicals were attached to eight specific pRNA sites without perturbing RNA folding and dimer formation, and a total of 17 internitroxide distances spanning the three-way junction were measured using Double Electron-Electron Resonance spectroscopy. The measured distances, together with steric chemical constraints, were used to select 3662 viable three-way junction models from a pool of 65 billion. The results reveal a similar conformation among the viable models, with two of the helices (HT and HL) adopting an acute bend. This is in contrast to a recently reported pRNA tetramer crystal structure, in which HT and HL stack onto each other linearly. The studies establish a new method for mapping global structures of complex RNA molecules, and provide information on pRNA conformation that aids investigations of phi29 packaging motor and developments of pRNA-based nanomedicine and nanomaterial.

  4. Modular assembly of chimeric phi29 packaging RNAs that support DNA packaging.

    Science.gov (United States)

    Fang, Yun; Shu, Dan; Xiao, Feng; Guo, Peixuan; Qin, Peter Z

    2008-08-08

    The bacteriophage phi29 DNA packaging motor is a protein/RNA complex that can produce strong force to condense the linear-double-stranded DNA genome into a pre-formed protein capsid. The RNA component, called the packaging RNA (pRNA), utilizes magnesium-dependent inter-molecular base-pairing interactions to form ring-shaped complexes. The pRNA is a class of non-coding RNA, interacting with phi29 motor proteins to enable DNA packaging. Here, we report a two-piece chimeric pRNA construct that is fully competent in interacting with partner pRNA to form ring-shaped complexes, in packaging DNA via the motor, and in assembling infectious phi29 virions in vitro. This is the first example of a fully functional pRNA assembled using two non-covalently interacting fragments. The results support the notion of modular pRNA architecture in the phi29 packaging motor.

  5. Loominguliste ja uuenduslike ideede rakendamisega seotud pinged / Maureen McLaughlin, Mary Ellen Vogt ; tõlkinud Tiina Nurm

    Index Scriptorium Estoniae

    McLaughlin, Maureen

    2000-01-01

    Selleks, et saaks toimuda tulemuslik ja uuenduslik aineõpetus, tuleb teadvustada ja ületada pinged, mis on seotud loovideede rakendamisega. Pingetekitajad, millega õpetajad uuenduslike õpetamis- ja õppimistoimingute rakendamisel võivad kokku puutuda, on järgmised : aeg, ainekavad, tekstid ja muud allikad, oletatav loovuse puudumine, ebakõla uuenduste ja reaalsuse vahel, õpilaste erinevus, tunni organiseerimine ja läbiviimine, hindamine ja hinnangu andmine, õpilaste usaldamine

  6. Contemporary Dances of Death in Niguliste Museum / Triin Hallas

    Index Scriptorium Estoniae

    Hallas, Triin, 1987-

    2009-01-01

    Bernt Notke 500. surma-aastapäevaga seoses Niguliste Muuseumis toimunud õpilaste haridusprogrammi raames valmisid tööd, mis esitavad tänapäeva versiooni Bernt Notke maalist "Surmatants". Õpilaste tööd olid 2009. a. väljas näitusel "Tänapäeva surmatants" ja Anu Männi kureeritud näitusel "Bernt Notke - uuenduste ja traditsioonide vahel"

  7. The risks of innovation and sustainability / Aivo Adamson

    Index Scriptorium Estoniae

    Adamson, Aivo

    2003-01-01

    Autor analüüsib Hansapanga näitel uuendustega kaasnevaid riske ja faktoreid, mis mõjutavad ettevõtte arengut. Uuenduste rakendamine peab olema mõõdetav ja juhitav ning on põhjendatud, kui nende tulemusena suureneb kasum, arendatakse uusi tooteid ja teenuseid, paraneb klienditeeninduse kvaliteet või luuakse töötajatele uusi võimalusi

  8. Targeted delivery of anti-coxsackievirus siRNAs using ligand-conjugated packaging RNAs.

    Science.gov (United States)

    Zhang, Huifang M; Su, Yue; Guo, Songchuan; Yuan, Ji; Lim, Travis; Liu, Jing; Guo, Peixuan; Yang, Decheng

    2009-09-01

    Coxsackievirus B3 (CVB3) is a common pathogen of myocarditis. We previously synthesized a siRNA targeting the CVB3 protease 2A (siRNA/2A) gene and achieved reduction of CVB3 replication by 92% in vitro. However, like other drugs under development, CVB3 siRNA faces a major challenge of targeted delivery. In this study, we investigated a novel approach to deliver CVB3 siRNAs to a specific cell population (e.g. HeLa cells containing folate receptor) using receptor ligand (folate)-linked packaging RNA (pRNA) from bacterial phage phi29. pRNA monomers can spontaneously form dimers and multimers under optimal conditions by base-pairing between their stem loops. By covalently linking a fluorescence-tag to folate, we delivered the conjugate specifically to HeLa cells without the need of transfection. We further demonstrated that pRNA covalently conjugated to siRNA/2A achieved an equivalent antiviral effect to that of the siRNA/2A alone. Finally, the drug targeted delivery was further evaluated by using pRNA monomers or dimers, which carried both the siRNA/2A and folate ligand and demonstrated that both of them strongly inhibited CVB3 replication. These data indicate that pRNA as a siRNA carrier can specifically deliver the drug to target cells via its ligand and specific receptor interaction and inhibit virus replication effectively.

  9. Construction of RNA-Quantum Dot Chimera for Nanoscale Resistive Biomemory Application.

    Science.gov (United States)

    Lee, Taek; Yagati, Ajay Kumar; Pi, Fengmei; Sharma, Ashwani; Choi, Jeong-Woo; Guo, Peixuan

    2015-07-28

    RNA nanotechnology offers advantages to construct thermally and chemically stable nanoparticles with well-defined shape and structure. Here we report the development of an RNA-QD (quantum dot) chimera for resistive biomolecular memory application. Each QD holds two copies of the pRNA three-way junction (pRNA-3WJ) of the bacteriophage phi29 DNA packaging motor. The fixed quantity of two RNAs per QD was achieved by immobilizing the pRNA-3WJ with a Sephadex aptamer for resin binding. Two thiolated pRNA-3WJ serve as two feet of the chimera that stand on the gold plate. The RNA nanostructure served as both an insulator and a mediator to provide defined distance between the QD and gold. Immobilization of the chimera nanoparticle was confirmed with scanning tunneling microscopy. As revealed by scanning tunneling spectroscopy, the conjugated pRNA-3WJ-QD chimera exhibited an excellent electrical bistability signal for biomolecular memory function, demonstrating great potential for the development of resistive biomolecular memory and a nano-bio-inspired electronic device for information processing and computing.

  10. Lihtsamatele töödele napib ladudes ja terminalides tööjõudu / Rivo Sarapik

    Index Scriptorium Estoniae

    Sarapik, Rivo, 1981-

    2005-01-01

    Ilmunud ka: Delovõje Vedomosti 2. nov. lk. 17. Teravnemas on terminali- ja laotööliste puudus, sest lihttöödele on keeruline töötajaid leida. Kommenteerivad: Andres Leht, Annika Lootus, Mait Miller ja Margus Tammaru

  11. Unusta, säilita, mäleta : kunstnikud materialiseerivad mälu / Laura Põld

    Index Scriptorium Estoniae

    Põld, Laura, 1984-

    2015-01-01

    Rahvusvaheline näitus "Tracing the Memory" Vaal galeriis 17. detsembrist 2014 kuni 17. jaanuarini 2015, kuraatorid Annika Toots ja Slavomira Ondrušová. Eksponeeriti Daniela Krajčová, Jan Nálevka, Katarina Poliačiková, Laura Tootsi ja Milan Vagač'i installatsioone. Kunstnike eluloolisi andmeid

  12. Coca-Cola : kasumi languses on süüdi Põhja-Euroopa kehv ilm / Raivo Sormunen

    Index Scriptorium Estoniae

    Sormunen, Raivo, 1976-

    2004-01-01

    Ilmunud ka: Delovõje Vedomosti 29. sept. lk. 6. Karastusjookide tootja Coca-Cola andis kasumihoiatuse. Ettevõtte majandustulemuste languse põhjustest. Diagramm: Coca-Cola aktsia aasta madalaimal tasemel. Vt. samas: Annika Matson. Coca-Cola jooksev kvartal Eestis saladus

  13. Eraeluline kunst / Johannes Saar

    Index Scriptorium Estoniae

    Saar, Johannes, 1965-

    1998-01-01

    Briti ja eesti kunstnike ühisnäitus "Private views" Rotermanni soolalaos. Eestit esindavad Mari Koort, Anu Juurak, Annika Tonts, Kiwa, Ene-Liis Semper, Inglismaad Pam Skelton. Naiste näitus naiseks olemise vaevadest. Kuraatorid Mare Tralla ja Pam Skelton. Ilmunud ka kogumikus "Päevast päeva", lk.141-143

  14. Riik koolitab Eesti doktoreid välismaal

    Index Scriptorium Estoniae

    2006-01-01

    Tänavu sai välisülikoolidesse doktoriõppe konkurssi korraldav SA Archimedes kaks taotlust tervishoiu erialalt. Riikliku koolitustellimuse välisdoktorantuuri kohtadel õpib praegu viis stipendiaati meditsiini valdkonnast: Annika Kits, Jana Jaal, Kristo Kask, Tarmo Tiido, Priit Eelmäe

  15. Fonoloogiast piiblitõlkeni. Mati Hindi juubelikogumik / Reili Argus

    Index Scriptorium Estoniae

    Argus, Reili, 1967-

    2012-01-01

    Tutvustus: Pühendusteos emeriitprofessor Mati Hindi 75. sünnipäevaks / koostanud ja toimetanud Reili Argus, Annika Hussar ja Tiina Rüütmaa. Tallinn : Tallinna Ülikool, 2012. (Tallinna Ülikooli eesti keele ja kultuuri instituudi toimetised ; 14)

  16. Nokia ja Sirmens panevad leivad ühte kappi / Lauri Matsulevitsh

    Index Scriptorium Estoniae

    Matsulevitsh, Lauri

    2006-01-01

    Nokia ja Siemens ühendavad võrguseadmete tootmise. Lisa: Annika Matson. Eesti võib võita uusi töökohti. Küsimustele vastab Avo Tihamäe. Diagramm: Ühinemisuudis pani aktsiad liikuma. Vt. samas: Sirje Rank. Analüütikud: Siemens võidab tehingust enam; Võrguseadmete tootjatel peal liitumistuhin

  17. Saarlased võitsid karaokevõistluse / Ene Kallas

    Index Scriptorium Estoniae

    Kallas, Ene

    2009-01-01

    15. veebruaril Tallinna restoranis Kännu Kõrts krooniti Annika Väälma ja Iivo Miil Eesti karaokekuningannaks ja -kuningaks, mõlemad saarlased osalevad ka 28. veebruaril Tallinnas Sokos Hotell Viru restoranis Merineitsi karaoke rahvusvahelisel võistulaulmisel "Finbaltic Karaoke King and Queen"

  18. Kolm nauditavat näidet nüüdisaegsest tantsust / Tiiu Laks

    Index Scriptorium Estoniae

    Laks, Tiiu, 1984-

    2011-01-01

    12. rahvusvahelisest Augusti tantsufestivalist. Brüsselis töötava ameerika koreograafi Daniel Linehani lavastustest "Montage for Three" ja "Not About Everything". Koreograaf David Wampachi (Pariis) lavastusest "Battement". Helsingis asutatud teatritrupi Oblivia triloogia "Entertainment Island" 2. osast, autorid-esitajad Anna Krzystek, Timo Fredriksson, Annika Tudeer

  19. Jam Session reloaded: Von der Marmeladenfabrik zum Kultur- und Kreativraum : Revitalisierung und Umnutzung der Zuegg-Marmeladenfabrik am Tribusplatz in Lana, Südtirol

    OpenAIRE

    Hillebrand, Annika

    2015-01-01

    von Annika Hillebrand Zusammenfassung in englischer Sprache Parallelt. [Übers. des Autors]: Jam Session Reloaded: From the jam factory to the cultural and creative space revitalization and redevelopment of Zuegg jam factory on Tribusplatz in Lana, South Tyrol Technische Universität Wien, Univ., Diplomarbeit, 2015

  20. Interaktiivne kunst Tartus

    Index Scriptorium Estoniae

    1999-01-01

    Paul Rodgersi kureeritud rahvusvaheline kohaspetsiifiline näitus 'Tartu anatoomikum' 22.-24. X Tartus Toomemäel vanas anatoomikumis. Osalesid 14 kunstnikku (loetletud) 8 riigist, Eestist Jaan Jaanisoo, Jüri Ojaver, Annika Tonts. Avamisel Aleksander Potolitsõni performance. Kunstiprojektiga kaasnes seminar

  1. Mastaabi ja miniatuuri kahekõne / Tanel Veenre

    Index Scriptorium Estoniae

    Veenre, Tanel, 1977-

    2010-01-01

    Metallikunstnik Urve Küttneri ja keraamik Annika Tederi näitus Viinistu Kunstimuuseumi tünnigaleriis 28. augustini 2010. Per Suntumi (Taani) ja Jaapani juurtega Kaori Juzu näitus Tallinnas A-Galeriis 16. augustini 2010

  2. Peotäis konisid varastatud autos?! / Janar Filippov

    Index Scriptorium Estoniae

    Filippov, Janar, 1976-

    2006-01-01

    Populaarne USA krimisari "C.S.I. : Kriminalistid" on mõjutanud Ameerika Ühendriikide kuritegevust ja kohtute tööd. Seda kas ja kuidas on sari mõjunud Eesti kuritegevuse trendidele, kommenteerib kriminalistikatalituse komissar Annika Lall

  3. Läbi tule

    Index Scriptorium Estoniae

    2004-01-01

    8.-18. IV 2004 Eesti Tarbekunsti- ja Disainimuuseumis Eesti Kunstiakadeemia keraamikaosakonna magistrantide grupinäitus "Läbi tule". Reeli Haamer, Kadri Kivi, Annika Vilippus, Ingrid Allik, Mathew Graziano ja Milvi Korela kaitsevad 12. IV oma magistritööd. 23. IV-15. VI näitus Leesi Ermi loomingust sarjas "Klassikud"

  4. Novel Seals and Specialty Component Attachment Mechanisms for Respiratory Protection System (RESPO 21)

    Science.gov (United States)

    1992-10-01

    use in a warm clima ,, Ao~ hccts rovcf rur % Cr. ugc na il oimnte nber o prna in ntting materil coupled to skin separat lc~oe tect wocn f h~i. Sae...the mask shell on the side vagina and cervix of a woman in labor , electrode meam &h thereof facing the face. said sealing tube having a fixed sealed

  5. 5'-Phospho-RNA Acceptor Specificity of GDP Polyribonucleotidyltransferase of Vesicular Stomatitis Virus in mRNA Capping.

    Science.gov (United States)

    Ogino, Minako; Ogino, Tomoaki

    2017-03-15

    The GDP polyribonucleotidyltransferase (PRNTase) domain of the multifunctional L protein of rhabdoviruses, such as vesicular stomatitis virus (VSV) and rabies virus, catalyzes the transfer of 5'-phospho-RNA (pRNA) from 5'-triphospho-RNA (pppRNA) to GDP via a covalent enzyme-pRNA intermediate to generate a 5'-cap structure (GpppA). Here, using an improved oligo-RNA capping assay with the VSV L protein, we showed that the Michaelis constants for GDP and pppAACAG (VSV mRNA-start sequence) are 0.03 and 0.4 μM, respectively. A competition assay between GDP and GDP analogues in the GpppA formation and pRNA transfer assay using GDP analogues as pRNA acceptors indicated that the PRNTase domain recognizes the C-2-amino group, but not the C-6-oxo group, N-1-hydrogen, or N-7-nitrogen, of GDP for the cap formation. 2,6-Diaminopurine-riboside (DAP), 7-deazaguanosine (7-deaza-G), and 7-methylguanosine (m 7 G) diphosphates efficiently accepted pRNA, resulting in the formation of DAPpppA, 7-deaza-GpppA, and m 7 GpppA (cap 0), respectively. Furthermore, either the 2'- or 3'-hydroxyl group of GDP was found to be required for efficient pRNA transfer. A 5'-diphosphate form of antiviral ribavirin weakly inhibited the GpppA formation but did not act as a pRNA acceptor. These results indicate that the PRNTase domain has a unique guanosine-binding mode different from that of eukaryotic mRNA capping enzyme, guanylyltransferase. IMPORTANCE mRNAs of nonsegmented negative-strand (NNS) RNA viruses, such as VSV, possess a fully methylated cap structure, which is required for mRNA stability, efficient translation, and evasion of antiviral innate immunity in host cells. GDP polyribonucleotidyltransferase (PRNTase) is an unconventional mRNA capping enzyme of NNS RNA viruses that is distinct from the eukaryotic mRNA capping enzyme, guanylyltransferase. In this study, we studied the pRNA acceptor specificity of VSV PRNTase using various GDP analogues and identified chemical groups of GDP as

  6. 5′-Phospho-RNA Acceptor Specificity of GDP Polyribonucleotidyltransferase of Vesicular Stomatitis Virus in mRNA Capping

    Science.gov (United States)

    Ogino, Minako

    2017-01-01

    ABSTRACT The GDP polyribonucleotidyltransferase (PRNTase) domain of the multifunctional L protein of rhabdoviruses, such as vesicular stomatitis virus (VSV) and rabies virus, catalyzes the transfer of 5′-phospho-RNA (pRNA) from 5′-triphospho-RNA (pppRNA) to GDP via a covalent enzyme-pRNA intermediate to generate a 5′-cap structure (GpppA). Here, using an improved oligo-RNA capping assay with the VSV L protein, we showed that the Michaelis constants for GDP and pppAACAG (VSV mRNA-start sequence) are 0.03 and 0.4 μM, respectively. A competition assay between GDP and GDP analogues in the GpppA formation and pRNA transfer assay using GDP analogues as pRNA acceptors indicated that the PRNTase domain recognizes the C-2-amino group, but not the C-6-oxo group, N-1-hydrogen, or N-7-nitrogen, of GDP for the cap formation. 2,6-Diaminopurine-riboside (DAP), 7-deazaguanosine (7-deaza-G), and 7-methylguanosine (m7G) diphosphates efficiently accepted pRNA, resulting in the formation of DAPpppA, 7-deaza-GpppA, and m7GpppA (cap 0), respectively. Furthermore, either the 2′- or 3′-hydroxyl group of GDP was found to be required for efficient pRNA transfer. A 5′-diphosphate form of antiviral ribavirin weakly inhibited the GpppA formation but did not act as a pRNA acceptor. These results indicate that the PRNTase domain has a unique guanosine-binding mode different from that of eukaryotic mRNA capping enzyme, guanylyltransferase. IMPORTANCE mRNAs of nonsegmented negative-strand (NNS) RNA viruses, such as VSV, possess a fully methylated cap structure, which is required for mRNA stability, efficient translation, and evasion of antiviral innate immunity in host cells. GDP polyribonucleotidyltransferase (PRNTase) is an unconventional mRNA capping enzyme of NNS RNA viruses that is distinct from the eukaryotic mRNA capping enzyme, guanylyltransferase. In this study, we studied the pRNA acceptor specificity of VSV PRNTase using various GDP analogues and identified chemical groups

  7. An RNA Domain Imparts Specificity and Selectivity to a Viral DNA Packaging Motor

    Science.gov (United States)

    Zhao, Wei; Jardine, Paul J.

    2015-01-01

    ABSTRACT During assembly, double-stranded DNA viruses, including bacteriophages and herpesviruses, utilize a powerful molecular motor to package their genomic DNA into a preformed viral capsid. An integral component of the packaging motor in the Bacillus subtilis bacteriophage ϕ29 is a viral genome-encoded pentameric ring of RNA (prohead RNA [pRNA]). pRNA is a 174-base transcript comprised of two domains, domains I and II. Early studies initially isolated a 120-base form (domain I only) that retains high biological activity in vitro; hence, no function could be assigned to domain II. Here we define a role for this domain in the packaging process. DNA packaging using restriction digests of ϕ29 DNA showed that motors with the 174-base pRNA supported the correct polarity of DNA packaging, selectively packaging the DNA left end. In contrast, motors containing the 120-base pRNA had compromised specificity, packaging both left- and right-end fragments. The presence of domain II also provides selectivity in competition assays with genomes from related phages. Furthermore, motors with the 174-base pRNA were restrictive, in that they packaged only one DNA fragment into the head, whereas motors with the 120-base pRNA packaged several fragments into the head, indicating multiple initiation events. These results show that domain II imparts specificity and stringency to the motor during the packaging initiation events that precede DNA translocation. Heteromeric rings of pRNA demonstrated that one or two copies of domain II were sufficient to impart this selectivity/stringency. Although ϕ29 differs from other double-stranded DNA phages in having an RNA motor component, the function provided by pRNA is carried on the motor protein components in other phages. IMPORTANCE During virus assembly, genome packaging involves the delivery of newly synthesized viral nucleic acid into a protein shell. In the double-stranded DNA phages and herpesviruses, this is accomplished by a powerful

  8. An RNA Domain Imparts Specificity and Selectivity to a Viral DNA Packaging Motor.

    Science.gov (United States)

    Zhao, Wei; Jardine, Paul J; Grimes, Shelley

    2015-12-01

    During assembly, double-stranded DNA viruses, including bacteriophages and herpesviruses, utilize a powerful molecular motor to package their genomic DNA into a preformed viral capsid. An integral component of the packaging motor in the Bacillus subtilis bacteriophage ϕ29 is a viral genome-encoded pentameric ring of RNA (prohead RNA [pRNA]). pRNA is a 174-base transcript comprised of two domains, domains I and II. Early studies initially isolated a 120-base form (domain I only) that retains high biological activity in vitro; hence, no function could be assigned to domain II. Here we define a role for this domain in the packaging process. DNA packaging using restriction digests of ϕ29 DNA showed that motors with the 174-base pRNA supported the correct polarity of DNA packaging, selectively packaging the DNA left end. In contrast, motors containing the 120-base pRNA had compromised specificity, packaging both left- and right-end fragments. The presence of domain II also provides selectivity in competition assays with genomes from related phages. Furthermore, motors with the 174-base pRNA were restrictive, in that they packaged only one DNA fragment into the head, whereas motors with the 120-base pRNA packaged several fragments into the head, indicating multiple initiation events. These results show that domain II imparts specificity and stringency to the motor during the packaging initiation events that precede DNA translocation. Heteromeric rings of pRNA demonstrated that one or two copies of domain II were sufficient to impart this selectivity/stringency. Although ϕ29 differs from other double-stranded DNA phages in having an RNA motor component, the function provided by pRNA is carried on the motor protein components in other phages. During virus assembly, genome packaging involves the delivery of newly synthesized viral nucleic acid into a protein shell. In the double-stranded DNA phages and herpesviruses, this is accomplished by a powerful molecular motor

  9. Tubin: The Parson of Reigi / Robert Layton

    Index Scriptorium Estoniae

    Layton, Robert

    1993-01-01

    Uuest heliplaadist "Tubin: The Parson of Reigi. Teo Maiste, Marika Eensalu, Ivo Kuusk, Annika Tõnuri, Urve Tauts, Leili Tammel, Tiit Tralla, Arvo Laid. Estonia Opera Company / Paul Mägi; Requiem for Fallen Soldiers. Urve Tauts, Talevaldis Deksnis, Urmas Leiten, Rein Tiido, Rein Roos. Estonian National Male Choir / Eri Klas. Ondine CD ODE 783-2 (2 discs: 116 min.). Recorded in association with the Estonian Cultural Foundation. Requiem - comparative version: Lund. Choral Soc., Järvi (9/88) CD 297

  10. Intiimsed pilgud (taas)avastatud ruumile

    Index Scriptorium Estoniae

    1998-01-01

    Eesti ja inglise kunstnike feministlik ühisprojekt "Private Views" 1. juunist Rotermanni soolalaos. Kuraatorid Mare Tralla, Pam Skelton. Näituse eesmärk - jälgida ruumikogemuse vahendamist eesti ja inglise kunstis. Keskmes naise koht ja suhe ruumiga 90ndate Euroopas. Kasutatud fotot, videot, arvutikunsti. Osalejad toodud - Eestist Anu Juurak, Eve Kiiler, Mari Koort, Ene-Liis Semper, Kiwa, Liina Siib, Annika Tonts, F.F.F.F., Mare Tralla

  11. Viron televisio- ja radiokeskus = Estonian National Broadcasting Building / Pentti Kareoja

    Index Scriptorium Estoniae

    Kareoja, Pentti

    2008-01-01

    Eesti Rahvusringhäälingu hoone arhitektuurikonkursi võitis Taani arhitektuuribüroo Nobel Arkitekter. Teise koha pälvis Soome arhitektuuribüroo Harris-Kjisik OY (arhitektid Trevor Harris, Taru Niskanen) töö "Mediatetris". Ergutuspreemia pälvis Pekka Lassila Arhitektuuribüroo (arhitektid Pekka Lassila, Janne Kantee, Annika Ritola, Tuomas Arnala) töö "Auteur"

  12. LHV üritas rikastuda infovargusega / Sirje Rank

    Index Scriptorium Estoniae

    Rank, Sirje, 1966-

    2005-01-01

    USA väärtpaberijärelvalve komisjon (SEC) süüdistab investeerimispanka LHV ja panga kahte töötajat salajase info abil väärtpaberitehingute tegemises. Diagramm: Aktsiatehingud. Kommenteerivad Sten Tamkivi ja Merlis Nõgene. Vt. samas: Kristjan Lepik: olümpiapronksiga olnuks parem kuulsaks saada; Annika Matson. Peek kasvatas oma vara kahe aastaga üle 70 korra

  13. Proof-of-principle that a decoy virus protects oncolytic measles virus against neutralizing antibodies

    OpenAIRE

    Xu C; Goß AV; Dorneburg C; Debatin KM; Wei J; Beltinger C

    2018-01-01

    Chun Xu,1,2,* Annika Verena Goß,1,* Carmen Dorneburg,1 Klaus-Michael Debatin,1 Jiwu Wei,2 Christian Beltinger1 1Department of Pediatrics and Adolescent Medicine, Section of Experimental Pediatric Oncology, University Medical Center Ulm, Ulm, Germany; 2Jiangsu Key Laboratory of Molecular Medicine, Medical School of Nanjing University, China *These authors contributed equally to this work Background: Attenuated oncolytic measles virus (OMV) is a promising antitumor agent in early-phase cl...

  14. 10.-14. II on Puhta Rõõmu alaliiduna tuntud...

    Index Scriptorium Estoniae

    2004-01-01

    Lõkerdus- ja tegevuskunstikollektiiv Naeruorgesster ehk Laffing ahkestra - dirigent Erki Kannus, lõkerdajad Ivika Kivi, Piret Räni, Merle Kannus, Maris Mändel, Marge Monko, Aino Sepp, Annika Kaljurand, Anya Ronacher - esines 11. II Stockholmis Moderna Museeti taasavamispidustuste raames, 12. II osaleti tegevuskunstiprogrammis "Source Live 2004". Aprillis oodatakse kollektiivi Varssavisse Zacheta galerii näitusele "Poisid ja tüdrukud" (16. II-4. IV), kus esitatakse Piret Räni ja Anu Vahtra videot "Punk.Fem.Collection"

  15. Characterization of bacteriophage KVP40 and T4 RNA ligase 2

    International Nuclear Information System (INIS)

    Yin Shenmin; Kiong Ho, C.; Miller, Eric S.; Shuman, Stewart

    2004-01-01

    Bacteriophage T4 RNA ligase 2 (Rnl2) exemplifies a subfamily of RNA strand-joining enzymes that includes the trypanosome RNA editing ligases. A homolog of T4 Rnl2 is encoded in the 244-kbp DNA genome of vibriophage KVP40. We show that the 335-amino acid KVP40 Rnl2 is a monomeric protein that catalyzes RNA end-joining through ligase-adenylate and RNA-adenylate (AppRNA) intermediates. In the absence of ATP, pre-adenylated KVP40 Rnl2 reacts with an 18-mer 5'-PO 4 single-strand RNA (pRNA) to form an 18-mer RNA circle. In the presence of ATP, Rnl2 generates predominantly AppRNA. Isolated AppRNA can be circularized by KVP40 Rnl2 in the absence of ATP. The reactivity of phage Rnl2 and the distribution of the products are affected by the length of the pRNA substrate. Whereas 18-mer and 15-mer pRNAs undergo intramolecular sealing by T4 Rnl2 to form monomer circles, a 12-mer pRNA is ligated intermolecularly to form dimers, and a 9-mer pRNA is unreactive. In the presence of ATP, the 15-mer and 12-mer pRNAs are converted to AppRNAs, but the 9-mer pRNA is not. A single 5' deoxynucleotide substitution of an 18-mer pRNA substrate has no apparent effect on the 5' adenylation or circularization reactions of T4 Rnl2. In contrast, a single deoxyribonucleoside at the 3' terminus strongly and selectively suppresses the sealing step, thereby resulting in accumulation of high levels of AppRNA in the absence of ATP. The ATP-dependent 'capping' of RNA with AMP by Rnl2 is reminiscent of the capping of eukaryotic mRNA with GMP by GTP:RNA guanylyltransferase and suggests an evolutionary connection between bacteriophage Rnl2 and eukaryotic RNA capping enzymes

  16. Töölepingu seaduse § 37 : kas tööandja võimalus oma äranägemise järgi töötasu vähendada või turvalise paindlikkuse rakendamise positiivne näide? / Annika Rosin

    Index Scriptorium Estoniae

    Rosin, Annika

    2013-01-01

    Millistel tingimustel on võimalik tööandjal ühepoolselt töötasu vähendada, kas ja kuidas on vastav reeglistik võrreldes varasemaga muutunud ning kas sellise võimaluse andmine on kooskõlas turvalise paindlikkuse kontseptsiooniga

  17. Töölepingu lõpetamine tööandja algatusel : (Eesti ja Ameerika Ühendriikide seadusandluse võrdlev käsitlus) : [bakalaureusetöö] / Annika Timusk ; Tartu Ülikool, õigusteaduskond ; juhendaja: Inge-Maret Orgo

    Index Scriptorium Estoniae

    Timusk, Annika

    2000-01-01

    Tööandja algatusel töölepingu lõpetamise üldiseloomustus ja lõpetamise alused, lõpetamine majanduslikel põhjustel, töötajaga seoses olevatel põhjustel; asjaolud, mis takistevad töölepingu lõpetamist

  18. Co-isolation of in vivo 32P-labeled specific transcripts and DNA without phenol extraction of nuclease digestion

    International Nuclear Information System (INIS)

    Hayes, S.; Hayes, C.; Brand, L.

    1981-01-01

    A method is described for isolation and quantitation of specific intact transcripts, for which a hybridization probe is available, from 32 P-labeled bacterial cells. The RNA is extracted in the absence of R Nase activity by incorporating an inert, physically removable R Nase inhibitor throughout the spheroplasting, cell lysis, and pronase digestion steps. [/sup 32/P]RNA is separated from [ 32 P]DNA, without recourse to phenol extraction of DNase treatment, on a Cs 2 SO/sub 4-/HCONH 2 step gradient in which the precipitated RNA forms a sharp band. Specific transcripts are purified from [ 32 P]RNA by physical separation of the transcript and hybridization probe using gel-exclusion chromatography. The gentleness of this technique enables the co-isolation of DNA and can facilitate the analysis of covalently joined RNA-DNA replication intermediates

  19. Characterization of the archaeal ribonuclease P proteins from Pyrococcus horikoshii OT3.

    Science.gov (United States)

    Terada, Atsushi; Honda, Takashi; Fukuhara, Hideo; Hada, Kazumasa; Kimura, Makoto

    2006-08-01

    Ribonuclease P (RNase P) is a ribonucleoprotein complex involved in the processing of the 5'-leader sequence of precursor tRNA (pre-tRNA). Our earlier study revealed that RNase P RNA (pRNA) and five proteins (PhoPop5, PhoRpp38, PhoRpp21, PhoRpp29, and PhoRpp30) in the hyperthermophilic archaeon Pyrococcus horikoshii OT3 reconstituted RNase P activity that exhibits enzymatic properties like those of the authentic enzyme. In present study, we investigated involvement of the individual proteins in RNase P activity. Two particles (R-3Ps), in which pRNA was mixed with three proteins, PhoPop5, PhoRpp30, and PhoRpp38 or PhoPop5, PhoRpp30, and PhoRpp21 showed a detectable RNase P activity, and five reconstituted particles (R-4Ps) composed of pRNA and four proteins exhibited RNase P activity, albeit at reduced level compared to that of the reconstituted particle (R-5P) composed of pRNA and five proteins. Time-course analysis of the RNase P activities of R-4Ps indicated that the R-4Ps lacking PhoPop5, PhoRpp21, or PhoRpp30 had virtually reduced activity, while omission of PhoRpp29 or PhoRpp38 had a slight effect on the activity. The results indicate that the proteins contribute to RNase P activity in order of PhoPop5 > PhoRpp30 > PhoRpp21 > PhoRpp29 > PhoRpp38. It was further found that R-4Ps showed a characteristic Mg2+ ion dependency approximately identical to that of R-5P. However, R-4Ps had optimum temperature of around at 55 degrees C which is lower than 70 degrees C for R-5P. Together, it is suggested that the P. horikoshii RNase P proteins are predominantly involved in optimization of the pRNA conformation, though they are individually dispensable for RNase P activity in vitro.

  20. CO₂-st on saanud oluline strateegiliste äriotsuste mõjutaja / interv. Axel Threfall

    Index Scriptorium Estoniae

    2008-01-01

    Ilmunud ka: Finansovõi Menedzhment : infovõpusk juuli nr. 26 lk. 10-13. Euroopa Liidu saastekaubanduse, saastekvootide hinna ja prognoosimise võimalikkuse üle arutlevad konsultatsioonifirma BeCitizen direktor Philippe Freund, Inteli Euroopa ja Lähis Ida treasury-operatsioonide asedirektor Tamiko Hutchinson ja Ahlstrom Corporationi treasury-operatsioonide direktor Annika Westerholm. Vt samas Lõviosa vabatahtlikest kvoodiostudest teevad erafirmad; Millest sõltub saastekvootide hind?; Lühike heitmekaubanduse sõnastik. Tabel: Eestile 2008-2012 eraldatud saastekvootide jagunemine

  1. Letter to the editor

    Directory of Open Access Journals (Sweden)

    Linde A

    2016-08-01

    Full Text Available Annika Linde, Amelie Plymoth Department of Medical Epidemiology and Biostatistics, Karolinska Institutet, Stockholm, Sweden It was with great interest that we noticed that other researchers are also interested in participant-collected nasal swabs to detect pathogens in acute respiratory infections, and we welcome the findings presented in the study by Vargas et al.1        Of importance is that already in the beginning of 2015, we showed in our research work that nasal self-sampling is feasible in large-scale surveillance of respiratory infections.2  View the original paper by Vargas and colleagues. 

  2. TÜ, TPÜ ja EHI uusi magistreid / Eda Tursk, Hille Roots, Heidi Meier

    Index Scriptorium Estoniae

    Tursk, Eda

    2004-01-01

    Tartu ülikooli eesti ja soome-ugri keeleteaduse osakonnas ning kirjanduse ja rahvaluule osakonnas kaitsesid 2003.a. magistritööd Niina Aasmäe, Piret Voll, Larissa Degel, Reet Hendrikson, Tiina Pai, Petar Kehayov, Anna Baidullina, Katrin Ennus, Kristi Jõesaar, Ell Vahtramäe, Lauri Sommer, Andreas Kalkun, Mirjam Hinrikus, Kristel Nõlvak. Tallinna Pedagoogikaülikoolis kaitsesid 2003.a. magistritööd Sirje Nootre, Merike Mägedi, Tiiu Koovit, Heidi Meier, Jaanika Stackhouse, Lilian Ossi, Annika Vamper, Marika Mikkor, Piret Õunapuu, Helin Puksand, Taimi Rosenberg. Eesti Humanitaarinstituudis kaitses 2003.a. magistritööd Merilin Miljan

  3. Intracellular delivery of poly(I:C) induces apoptosis of fibroblast-like synoviocytes via an unknown dsRNA sensor

    Energy Technology Data Exchange (ETDEWEB)

    Karpus, Olga N.; Hsiao, Cheng-Chih; Kort, Hanneke de; Tak, Paul P.; Hamann, Jörg, E-mail: j.hamann@amc.uva.nl

    2016-08-26

    Fibroblast-like synoviocytes (FLS) express functional membranous and cytoplasmic sensors for double-stranded (ds)RNA. Notably, FLS undergo apoptosis upon transfection with the synthetic dsRNA analog poly(I:C). We here studied the mechanism of intracellular poly(I:C) recognition and subsequent cell death in FLS. FLS responded similarly to poly(I:C) or 3pRNA transfection; however, only intracellular delivery of poly(I:C) induced significant cell death, accompanied by upregulation of pro-apoptotic proteins Puma and Noxa, caspase 3 cleavage, and nuclear segregation. Knockdown of the DExD/H-box helicase MDA5 did not affect the response to intracellular poly(I:C); in contrast, knockdown of RIG-I abrogated the response to 3pRNA. Knockdown of the downstream adaptor proteins IPS, STING, and TRIF or inhibition of TBK1 did not affect the response to intracellular poly(I:C), while knockdown of IFNAR blocked intracellular poly(I:C)-mediated signaling and cell death. We conclude that a so far unknown intracellular sensor recognizes linear dsRNA and induces apoptosis in FLS. - Highlights: • Intracellular poly(I:C) and 3pRNA evoke immune responses in FLS. • Only intracellular delivery of poly(I:C) induces FLS apoptosis. • FLS do not require MDA5 for their response to intracellular poly(I:C). • FLS respond to intracellular poly(I:C) independent of IPS and STING. • An unknown intracellular sensor recognizes linear dsRNA in FLS.

  4. Real-time quantitative PCR for retrovirus-like particle quantification in CHO cell culture.

    Science.gov (United States)

    de Wit, C; Fautz, C; Xu, Y

    2000-09-01

    Chinese hamster ovary (CHO) cells have been widely used to manufacture recombinant proteins intended for human therapeutic uses. Retrovirus-like particles, which are apparently defective and non-infectious, have been detected in all CHO cells by electron microscopy (EM). To assure viral safety of CHO cell-derived biologicals, quantification of retrovirus-like particles in production cell culture and demonstration of sufficient elimination of such retrovirus-like particles by the down-stream purification process are required for product market registration worldwide. EM, with a detection limit of 1x10(6) particles/ml, is the standard retrovirus-like particle quantification method. The whole process, which requires a large amount of sample (3-6 litres), is labour intensive, time consuming, expensive, and subject to significant assay variability. In this paper, a novel real-time quantitative PCR assay (TaqMan assay) has been developed for the quantification of retrovirus-like particles. Each retrovirus particle contains two copies of the viral genomic particle RNA (pRNA) molecule. Therefore, quantification of retrovirus particles can be achieved by quantifying the pRNA copy number, i.e. every two copies of retroviral pRNA is equivalent to one retrovirus-like particle. The TaqMan assay takes advantage of the 5'-->3' exonuclease activity of Taq DNA polymerase and utilizes the PRISM 7700 Sequence Detection System of PE Applied Biosystems (Foster City, CA, U.S.A.) for automated pRNA quantification through a dual-labelled fluorogenic probe. The TaqMan quantification technique is highly comparable to the EM analysis. In addition, it offers significant advantages over the EM analysis, such as a higher sensitivity of less than 600 particles/ml, greater accuracy and reliability, higher sample throughput, more flexibility and lower cost. Therefore, the TaqMan assay should be used as a substitute for EM analysis for retrovirus-like particle quantification in CHO cell

  5. Proteomic Analysis of a Global Regulator GacS Sensor Kinase in the Rhizobacterium, Pseudomonas chlororaphis O6

    Directory of Open Access Journals (Sweden)

    Chul Hong Kim

    2014-06-01

    Full Text Available The GacS/GacA system in the root colonizer Pseudomonas chlororaphis O6 is a key regulator of many traits relevant to the biocontrol function of this bacterium. Proteomic analysis revealed 12 proteins were down-regulated in a gacS mutant of P. chlororaphis O6. These GacS-regulated proteins functioned in combating oxidative stress, cell signaling, biosynthesis of secondary metabolism, and secretion. The extent of regulation was shown by real-time RT-PCR to vary between the genes. Mutants of P. chlororaphis O6 were generated in two GacS-regulated genes, trpE, encoding a protein involved in tryptophan synthesis, and prnA, required for conversion of tryptophan to the antimicrobial compound, pyrrolitrin. Failure of the trpE mutant to induce systemic resistance in tobacco against a foliar pathogen causing soft rot, Pectobacterium carotovorum SCCI, correlated with reduced colonization of root surfaces implying an inadequate supply of tryptophan to support growth. Although colonization was not affected by mutation in the prnA gene, induction of systemic resistance was reduced, suggesting that pyrrolnitrin was an activator of plant resistance as well as an antifungal agent. Study of mutants in the other GacS-regulated proteins will indicate further the features required for biocontrol-activity in this rhizobacterium.

  6. Stimulation of StAR expression by cAMP is controlled by inhibition of highly inducible SIK1 via CRTC2, a co-activator of CREB.

    Science.gov (United States)

    Lee, Jinwoo; Tong, Tiegang; Takemori, Hiroshi; Jefcoate, Colin

    2015-06-15

    In mouse steroidogenic cells the activation of cholesterol metabolism is mediated by steroidogenic acute regulatory protein (StAR). Here, we visualized a coordinated regulation of StAR transcription, splicing and post-transcriptional processing, which are synchronized by salt inducible kinase (SIK1) and CREB-regulated transcription coactivator (CRTC2). To detect primary RNA (pRNA), spliced primary RNA (Sp-RNA) and mRNA in single cells, we generated probe sets by using fluorescence in situ hybridization (FISH). These methods allowed us to address the nature of StAR gene expression and to visualize protein-nucleic acid interactions through direct detection. We show that SIK1 represses StAR expression in Y1 adrenal and MA10 testis cells through inhibition of processing mediated by CRTC2. Digital image analysis matches qPCR analyses of the total cell culture. Evidence is presented for spatially separate accumulation of StAR pRNA and Sp-RNA at the gene loci in the nucleus. These findings establish that cAMP, SIK and CRTC mediate StAR expression through activation of individual StAR gene loci. Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

  7. Läänemeresoomlaste keele- ja kultuurikontaktidest laensõnade leviku põhjal

    Directory of Open Access Journals (Sweden)

    Vilja Oja

    2011-05-01

    Full Text Available Sõnavara uuenduste põhjusi ja levikuteid tuleb enamasti otsida keelevälistest teguritest – kultuurikontaktidest ning neid mõjutavatest asjaoludest. See, et läänemeresoomlased on elanud mitme muukeelse võimu all, peegeldub otseselt sõnavaras. Millisest keelest sõna laenatakse, sõltub ka mõiste sisust ja laenamise ajast. Keskajal tõi palju saksa laene Eesti- ja Liivimaale hansakaubandus. Põllundusega seostuv sõnavara näitab ühtlasi aega ja suunda, kust ja millal mingi viljeluskultuur võis siinseile aladele tulla. Artiklis analüüsitakse indoeuroopa laensõnade levikut läänemeresoome murretes Euroopa keelte atlase (ALE ja läänemeresoome keeleatlase (ALFE materjali põhjal. Kaardid on aidanud mõnegi sõna päritolu määratleda. http://dx.doi.org/10.5128/ERYa7.08

  8. The illusion of fame: how the nonfamous become famous.

    Science.gov (United States)

    Landau, Joshua D; Leed, Stacey A

    2012-01-01

    This article reports 2 experiments in which nonfamous faces were paired with famous (e.g., Oprah Winfrey) or semifamous (e.g., Annika Sorenstam) faces during an initial orienting task. In Experiment 1, the orienting task directed participants to consider the relationship between the paired faces. In Experiment 2, participants considered distinctive qualities of the paired faces. Participants then judged the fame level of old and new nonfamous faces, semifamous faces, and famous faces. Pairing a nonfamous face with a famous face resulted in a higher fame rating than pairing a nonfamous face with a semifamous face. The fame attached to the famous people was misattributed to their nonfamous partners. We discuss this pattern of results in the context of current theoretical explanations of familiarity misattributions.

  9. suPAR associates to glucose metabolic aberration during glucose stimulation in HIV-infected patients on HAART

    DEFF Research Database (Denmark)

    Andersen, Ove; Eugen-Olsen, Jesper; Kofoed, Kristian

    2008-01-01

    extend these findings by investigating the association of suPAR to glucose metabolic insufficiency during an oral glucose challenge (OGTT). METHODS: In 16 HIV-infected patients with lipodystrophy and 15 HIV-infected patients without lipodystrophy, glucose tolerance, insulin sensitivity (ISI......PAR correlated inversely with ISI(composite) and positively with 2h plasma glucose, fasting insulin secretion, fasting intact proinsulin and FFA level during the OGTT (all P...-RNA, duration of HIV infection), and dyslipidemia (plasma total cholesterol, triglyceride and free fatty acid level during the OGTT) were included, suPAR remained a significant marker of glucose tolerance and insulin sensitivity. Plasma suPAR exhibited a small CV (11%) during the 3h OGTT. CONCLUSIONS: su...

  10. Chitosan/siRNA nanoparticles encapsulated in PLGA nanofibers for siRNA delivery

    DEFF Research Database (Denmark)

    Chen, Menglin; Gao, Shan; Dong, Mingdong

    2012-01-01

    Composite nanofibers of biodegradable poly(d,l-lactic-co-glycolic acid) (PLGA) encapsulating chitosan/siRNA nanoparticles (NPs) were prepared by electrospinning. Acidic/alkaline hydrolysis and a bulk/surface degradation mechanism were investigated in order to achieve an optimized release profile...... for prolonged and efficient gene silencing. Thermo-controlled AFM in situ imaging not only revealed the integrity of the encapsulated chitosan/siRNA polyplex but also shed light on the decreasing Tg of PLGA on the fiber surfaces during release. A triphasic release profile based on bulk erosion was obtained at p......RNA transfection, where the encapsulated chitosan/siRNA NPs exhibited up to 50% EGFP gene silencing activity after 48 h post-transfection on H1299 cells....

  11. Acclimatization to 4100 m does not change capillary density or mRNA expression of potential angiogenesis regulatory factors in human skeletal muscle

    DEFF Research Database (Denmark)

    Lundby, Carsten; Pilegaard, Henriette; Andersen, Jesper L.

    2004-01-01

    growth factor (VEGF), a known target gene for hypoxia inducible factor 1 (HIF-1). We hypothesised that prolonged exposure to high altitude increases muscle capillary density and that this can be explained by an enhanced HIF-1alpha expression inducing an increase in VEGF expression. We measured mRNA...... or VEGF mRNA was not changed with prolonged hypoxic exposure in SLR, and both genes were similarly expressed in SLR and HAN. In SLR, whole body mass, mean muscle fibre area and capillary to muscle fibre ratio remained unchanged during acclimatization. The capillary to fibre ratio was lower in HAN than...... in SLR (2.4+/-0.1 vs 3.6+/-0.2; PRNA expression and capillary density are not significantly increased by 8 weeks of exposure to high altitude and are not increased in Aymara high-altitude natives compared with sea level residents....

  12. Targeting the cytosolic innate immune receptors RIG-I and MDA5 effectively counteracts cancer cell heterogeneity in glioblastoma.

    Science.gov (United States)

    Glas, Martin; Coch, Christoph; Trageser, Daniel; Dassler, Juliane; Simon, Matthias; Koch, Philipp; Mertens, Jerome; Quandel, Tamara; Gorris, Raphaela; Reinartz, Roman; Wieland, Anja; Von Lehe, Marec; Pusch, Annette; Roy, Kristin; Schlee, Martin; Neumann, Harald; Fimmers, Rolf; Herrlinger, Ulrich; Brüstle, Oliver; Hartmann, Gunther; Besch, Robert; Scheffler, Björn

    2013-06-01

    Cellular heterogeneity, for example, the intratumoral coexistence of cancer cells with and without stem cell characteristics, represents a potential root of therapeutic resistance and a significant challenge for modern drug development in glioblastoma (GBM). We propose here that activation of the innate immune system by stimulation of innate immune receptors involved in antiviral and antitumor responses can similarly target different malignant populations of glioma cells. We used short-term expanded patient-specific primary human GBM cells to study the stimulation of the cytosolic nucleic acid receptors melanoma differentiation-associated gene 5 (MDA5) and retinoic acid-inducible gene I (RIG-I). Specifically, we analyzed cells from the tumor core versus "residual GBM cells" derived from the tumor resection margin as well as stem cell-enriched primary cultures versus specimens without stem cell properties. A portfolio of human, nontumor neural cells was used as a control for these studies. The expression of RIG-I and MDA5 could be induced in all of these cells. Receptor stimulation with their respective ligands, p(I:C) and 3pRNA, led to in vitro evidence for an effective activation of the innate immune system. Most intriguingly, all investigated cancer cell populations additionally responded with a pronounced induction of apoptotic signaling cascades revealing a second, direct mechanism of antitumor activity. By contrast, p(I:C) and 3pRNA induced only little toxicity in human nonmalignant neural cells. Granted that the challenge of effective central nervous system (CNS) delivery can be overcome, targeting of RIG-I and MDA5 could thus become a quintessential strategy to encounter heterogeneous cancers in the sophisticated environments of the brain. Copyright © 2013 AlphaMed Press.

  13. Dose dependent activation of retinoic acid-inducible gene-I promotes both proliferation and apoptosis signals in human head and neck squamous cell carcinoma.

    Directory of Open Access Journals (Sweden)

    Jingzhou Hu

    Full Text Available The retinoic-acid-inducible gene (RIG-like receptor (RLR family proteins are major pathogen reorganization receptors (PRR responsible for detection of viral RNA, which initiates antiviral response. Here, we evaluated the functional role of one RLR family member, RIG-I, in human head and neck squamous cell carcinoma (HNSCC. RIG-I is abundantly expressed both in poorly-differentiated primary cancer and lymph node metastasis, but not in normal adjacent tissues. Activation of RIG-I by transfection with low dose of 5'-triphosphate RNA (3p-RNA induces low levels of interferon and proinflammatory cytokines and promotes NF-κB- and Akt-dependent cell proliferation, migration and invasion. In contrast, activation of RIG-I by a high dose of 3p-RNA induces robust mitochondria-derived apoptosis accompanied by decreased activation of Akt, which is independent of the interferon and TNFα receptor, but can be rescued by over-expression of constitutively active Akt. Furthermore, co-immunoprecipitation experiments indicate that the CARD domain of RIG-I is essential for inducing apoptosis by interacting with caspase-9. Together, our results reveal a dual role of RIG-I in HNSCC through regulating activation of Akt, in which RIG-I activation by low-dose viral dsRNA increases host cell survival, whereas higher level of RIG-I activation leads to apoptosis. These findings highlight the therapeutic potential of dsRNA mediated RIG-I activation in the treatment of HNSCC.

  14. 1,25 dihydroxyvitamin D3 (1,25) regulation of c-myc mRNA in HL-60 leukemia cells

    International Nuclear Information System (INIS)

    Simpson, R.U.; Bresnick, E.H.; Begley, D.A.

    1986-01-01

    Recently, 1,25 was shown to induce differentiation and decrease c-myc levels in HL-60 cells. The authors have confirmed these observations by RNA dot blot analysis. Cells treated with 50 nM 1,25 for 4, 24 and 48 hr showed c-myc mRNA levels of 26, 17 and 15% of control respectively. β-Actin mRNA levels were not altered. To ascertain whether 1, 25 regulated c-myc transcriptionally, an HL-60 nuclear RNA runoff assay was developed. Assay of total nuclei transcriptional activity revealed that 50% of RNA elongation was α-amanitin (0.8 μg/ml) sensitive and was linear with nuclei concentration (0.1-1 x 10 7 nuclei). 1,25 (50 nM) treated (45-96 hr) cells had decreased (approx.40%) total transcription rate relative to control. Decreased total RNA synthesis occurred concomitant with NBT reducing activity. 32 P-RNA runoff transcripts from HL-60 nuclei were hybridized to excess (5 μg DNA was excess) Pst I linearized c-myc and β-actin clones (in pBR322) immobilized on nitrocellulose filter. 32 P-RNA input from 2 x 10 6 to 2 x 10 7 cpm yielded linear hybridization signal. Analysis of blot dot intensity revealed no difference in transcription of c-myc in nuclei from 1,25 dosed or control cells. (myc/actin ratios: 1,25 (50 nM, 72 hr) =1.1 +/- 0.3 and control (72 hr) = 1.0, N=3 or 2 or 3 dots ea). These preliminary data suggest 1,25 does not affect c-myc transcription in HL-60 nuclei and may regulate c-myc mRNA post-transcriptionally

  15. Endothelial RIG-I activation impairs endothelial function

    Energy Technology Data Exchange (ETDEWEB)

    Asdonk, Tobias, E-mail: tobias.asdonk@ukb.uni-bonn.de [Department of Medicine/Cardiology, University of Bonn, Sigmund-Freud-Str. 25, 53105 Bonn (Germany); Motz, Inga; Werner, Nikos [Department of Medicine/Cardiology, University of Bonn, Sigmund-Freud-Str. 25, 53105 Bonn (Germany); Coch, Christoph; Barchet, Winfried; Hartmann, Gunther [Institute for Clinical Chemistry and Clinical Pharmacology, University of Bonn, Sigmund-Freud-Str. 25, 53105 Bonn (Germany); Nickenig, Georg; Zimmer, Sebastian [Department of Medicine/Cardiology, University of Bonn, Sigmund-Freud-Str. 25, 53105 Bonn (Germany)

    2012-03-30

    Highlights: Black-Right-Pointing-Pointer RIG-I activation impairs endothelial function in vivo. Black-Right-Pointing-Pointer RIG-I activation alters HCAEC biology in vitro. Black-Right-Pointing-Pointer EPC function is affected by RIG-I stimulation in vitro. -- Abstract: Background: Endothelial dysfunction is a crucial part of the chronic inflammatory atherosclerotic process and is mediated by innate and acquired immune mechanisms. Recent studies suggest that pattern recognition receptors (PRR) specialized in immunorecognition of nucleic acids may play an important role in endothelial biology in a proatherogenic manner. Here, we analyzed the impact of endothelial retinoic acid inducible gene I (RIG-I) activation upon vascular endothelial biology. Methods and results: Wild type mice were injected intravenously with 32.5 {mu}g of the RIG-ligand 3pRNA (RNA with triphosphate at the 5 Prime end) or polyA control every other day for 7 days. In 3pRNA-treated mice, endothelium-depended vasodilation was significantly impaired, vascular oxidative stress significantly increased and circulating endothelial microparticle (EMP) numbers significantly elevated compared to controls. To gain further insight in RIG-I dependent endothelial biology, cultured human coronary endothelial cells (HCAEC) and endothelial progenitor cells (EPC) were stimulated in vitro with 3pRNA. Both cells types express RIG-I and react with receptor upregulation upon stimulation. Reactive oxygen species (ROS) formation is enhanced in both cell types, whereas apoptosis and proliferation is not significantly affected in HCAEC. Importantly, HCAEC release significant amounts of proinflammatory cytokines in response to RIG-I stimulation. Conclusion: This study shows that activation of the cytoplasmatic nucleic acid receptor RIG-I leads to endothelial dysfunction. RIG-I induced endothelial damage could therefore be an important pathway in atherogenesis.

  16. Endothelial RIG-I activation impairs endothelial function

    International Nuclear Information System (INIS)

    Asdonk, Tobias; Motz, Inga; Werner, Nikos; Coch, Christoph; Barchet, Winfried; Hartmann, Gunther; Nickenig, Georg; Zimmer, Sebastian

    2012-01-01

    Highlights: ► RIG-I activation impairs endothelial function in vivo. ► RIG-I activation alters HCAEC biology in vitro. ► EPC function is affected by RIG-I stimulation in vitro. -- Abstract: Background: Endothelial dysfunction is a crucial part of the chronic inflammatory atherosclerotic process and is mediated by innate and acquired immune mechanisms. Recent studies suggest that pattern recognition receptors (PRR) specialized in immunorecognition of nucleic acids may play an important role in endothelial biology in a proatherogenic manner. Here, we analyzed the impact of endothelial retinoic acid inducible gene I (RIG-I) activation upon vascular endothelial biology. Methods and results: Wild type mice were injected intravenously with 32.5 μg of the RIG-ligand 3pRNA (RNA with triphosphate at the 5′end) or polyA control every other day for 7 days. In 3pRNA-treated mice, endothelium-depended vasodilation was significantly impaired, vascular oxidative stress significantly increased and circulating endothelial microparticle (EMP) numbers significantly elevated compared to controls. To gain further insight in RIG-I dependent endothelial biology, cultured human coronary endothelial cells (HCAEC) and endothelial progenitor cells (EPC) were stimulated in vitro with 3pRNA. Both cells types express RIG-I and react with receptor upregulation upon stimulation. Reactive oxygen species (ROS) formation is enhanced in both cell types, whereas apoptosis and proliferation is not significantly affected in HCAEC. Importantly, HCAEC release significant amounts of proinflammatory cytokines in response to RIG-I stimulation. Conclusion: This study shows that activation of the cytoplasmatic nucleic acid receptor RIG-I leads to endothelial dysfunction. RIG-I induced endothelial damage could therefore be an important pathway in atherogenesis.

  17. Stress-related neuropeptides and alcoholism: CRH, NPY and beyond

    Science.gov (United States)

    Ciccocioppo, Roberto; Gehlert, Donald R.; Ryabinin, Andrey; Kaur, Simranjit; Cippitelli, Andrea; Thorsell, Annika; Lê, Anh D.; Hipskind, Philip A.; Hamdouchi, Chafiq; Lu, Jianliang; Hembre, Erik J.; Cramer, Jeffrey; Song, Min; McKinzie, David; Morin, Michelle; Economidou, Daina; Stopponi, Serena; Cannella, Nazzareno; Braconi, Simone; Kallupi, Marsida; de Guglielmo, Giordano; Massi, Maurizio; George, David T.; Gilman, Jody; Hersh, Jacqueline; Tauscher, Johannes T.; Hunt, Stephen P.; Hommer, Daniel; Heilig, Markus

    2009-01-01

    This article summarizes the proceedings of a symposium held at the conference on “Alcoholism and Stress: A Framework for Future Treatment Strategies” in Volterra, Italy, May 6–9, 2008. Chaired by Markus Heilig and Roberto Ciccocioppo, this symposium offered a forum for the presentation of recent data linking neuropetidergic neurotransmission to the regulation of different alcohol related behaviours in animals and in humans. Dr. Donald Gehlert described the development of a new corticotrophin releasing factor (CRH) receptor 1 antagonist and showed its efficacy in reducing alcohol consumption and stress-induced relapse in different animal models of alcohol abuse. Dr. Andrey Ryabinin reviewed recent findings in his laboratory indicating a role of the urocortin 1 (Ucn) receptor system in the regulation of alcohol intake. Dr. Annika Thorsell showed data supporting the significance of the neuropetide Y (NPY) receptor system in the modulation of behaviours associated with a history of ethanol intoxication. Dr. Roberto Ciccocioppo focused his presentation on the nociceptin/orphanin FQ (N/OFQ) receptors as treatment targets for alcoholism. Finally, Dr. Markus Heilig showed recent preclinical and clinical evidence suggesting that neurokinin 1 (NK1) antagonism may represent a promising new treatment for alcoholism. Collectively, these investigators highlighted the significance of neuropeptidergic neurotransmission in the regulation of neurobiological mechanisms of alcohol addiction. Data also revealed the importance of these systems as treatment targets for the development of new medication for alcoholism. PMID:19913192

  18. New Nordic Exceptionalism

    DEFF Research Database (Denmark)

    Danbolt, Mathias

    2016-01-01

    At the 2009 Nordic Culture Forum summit in Berlin that centered on the profiling and branding of the Nordic region in a globalized world, one presenter stood out from the crowd. The lobbyist Annika Sigurdardottir delivered a speech that called for the establishment of “The United Nations of Norden...... that have been central to the debates on the branding of Nordicity over the last decades: on the one hand, the discourse of “Nordic exceptionalism,” that since the 1960s has been central to the promotion of a Nordic political, socio-economic, and internationalist “third way” model, and, on the other hand......, the discourse on the “New Nordic,” that emerged out of the New Nordic Food-movement in the early 2000s, and which has given art and culture a privileged role in the international re-fashioning of the Nordic brand. Through an analysis of Kim and Einhorn’s United Nations of Norden (UNN)-performance, the article...

  19. The Rabies Virus L Protein Catalyzes mRNA Capping with GDP Polyribonucleotidyltransferase Activity

    Directory of Open Access Journals (Sweden)

    Minako Ogino

    2016-05-01

    Full Text Available The large (L protein of rabies virus (RABV plays multiple enzymatic roles in viral RNA synthesis and processing. However, none of its putative enzymatic activities have been directly demonstrated in vitro. In this study, we expressed and purified a recombinant form of the RABV L protein and verified its guanosine 5′-triphosphatase and GDP polyribonucleotidyltransferase (PRNTase activities, which are essential for viral mRNA cap formation by the unconventional mechanism. The RABV L protein capped 5′-triphosphorylated but not 5′-diphosphorylated RABV mRNA-start sequences, 5′-AACA(C/U, with GDP to generate the 5′-terminal cap structure G(5′ppp(5′A. The 5′-AAC sequence in the substrate RNAs was found to be strictly essential for RNA capping with the RABV L protein. Furthermore, site-directed mutagenesis showed that some conserved amino acid residues (G1112, T1170, W1201, H1241, R1242, F1285, and Q1286 in the PRNTase motifs A to E of the RABV L protein are required for cap formation. These findings suggest that the putative PRNTase domain in the RABV L protein catalyzes the rhabdovirus-specific capping reaction involving covalent catalysis of the pRNA transfer to GDP, thus offering this domain as a target for developing anti-viral agents.

  20. Initiation of poliovirus plus-strand RNA synthesis in a membrane complex of infected HeLa cells

    International Nuclear Information System (INIS)

    Takeda, N.; Kuhn, R.J.; Yang, C.F.; Takegami, T.; Wimmer, E.

    1986-01-01

    An in vitro poliovirus RNA-synthesizing system derived from a crude membrance fraction of infected HeLa cells was used to analyze the mechanism of initiation of poliovirus plus-strand RNA synthesis. This system contains an activity that synthesizes the nucleotidyl proteins VPg-pU and VPg-pUpU. These molecules represent the 5'-terminal structure of nascent RNA molecules and of virion RNA. The membranous replication complex is also capable of synthesizing mucleotidyl proteins containing nine or more of the poliovirus 5'-proximal nucleotides as assayed by the formation of the RNase T 1 -resistant oligonucleotide VPg-pUUAAAACAGp or by fingerprint analysis of the in vitro-synthesized 32 P-RNA. Incubation of preformed VPg-pUpU with unlabeled nucleoside triphosphates resulted in the formation of VPg-pUUAAAACAGp. This reaction, which appeared to be an elongation of VPg-pUpU, was stimulated by the addition of a soluble fraction (S-10) obtained from uninfected HeLa cells. Preformed VPg-pU could be chased into VPg-pUpU in the presence of UTP. The data are consistent with a model that VPg-pU can function as a primer for poliovirus plus-strand RNA synthesis in the membranous replication complex and that the elongation reaction may be stimulated by a host cellular factor

  1. Synthesis of retinoid vitamin A-vitamin B6 conjugate analogues for antiviral chemotherapy

    International Nuclear Information System (INIS)

    Kesel, Andreas J.

    2003-01-01

    The synthesis of retinoid vitamin A-vitamin B 6 conjugate analogues from a vitamin B 6 coenzyme analogue and putative HIV-1 trans-activating transcriptional regulatory protein Tat antagonist (Z)-5 ' -O-phosphono-pyridoxylidenerhodanine (B6PR) monosodium salt hemiheptadecahydrate [(Z)-B6PRNa8.5H 2 O] is discussed here. All-trans-retinoic acid (ATRA) is coupled to B6PR by a modified Stork enamine acylation. It results in a product library of more than eight compounds, each with at least one intact all-trans or 13-cis vitamin A double bond system. This yellow oily concentrate mixture was subjected to matrix-assisted laser desorption/ionization-time-of-flight (MALDI-ToF) mass spectrometry (MS), UV/VIS-spectrophotometry, and proton nuclear magnetic resonance spectroscopy ( 1 H-NMR). The chemical structures of six components of the concentrate mixture could be established by combination of these analytical methods. The two main components are 65% 2 ' C,3O-(all-trans-retinylidyne)B6PT (B6RA) and 25% 2 ' C-(all-trans-retinoyl)B6PT, chemically derived from (5RS)-5-(5 ' -O-phosphono-pyridoxyl)-2,4-thiazolidinedione (B6PT). This new retinoid selection could be of further interest in antiviral applications, especially treating conditions caused by RNA viruses like HIV

  2. The Rabies Virus L Protein Catalyzes mRNA Capping with GDP Polyribonucleotidyltransferase Activity.

    Science.gov (United States)

    Ogino, Minako; Ito, Naoto; Sugiyama, Makoto; Ogino, Tomoaki

    2016-05-21

    The large (L) protein of rabies virus (RABV) plays multiple enzymatic roles in viral RNA synthesis and processing. However, none of its putative enzymatic activities have been directly demonstrated in vitro. In this study, we expressed and purified a recombinant form of the RABV L protein and verified its guanosine 5'-triphosphatase and GDP polyribonucleotidyltransferase (PRNTase) activities, which are essential for viral mRNA cap formation by the unconventional mechanism. The RABV L protein capped 5'-triphosphorylated but not 5'-diphosphorylated RABV mRNA-start sequences, 5'-AACA(C/U), with GDP to generate the 5'-terminal cap structure G(5')ppp(5')A. The 5'-AAC sequence in the substrate RNAs was found to be strictly essential for RNA capping with the RABV L protein. Furthermore, site-directed mutagenesis showed that some conserved amino acid residues (G1112, T1170, W1201, H1241, R1242, F1285, and Q1286) in the PRNTase motifs A to E of the RABV L protein are required for cap formation. These findings suggest that the putative PRNTase domain in the RABV L protein catalyzes the rhabdovirus-specific capping reaction involving covalent catalysis of the pRNA transfer to GDP, thus offering this domain as a target for developing anti-viral agents.

  3. Sorafenib inhibits tumor growth and vascularization of rhabdomyosarcoma cells by blocking IGF-1R-mediated signaling

    Directory of Open Access Journals (Sweden)

    Wessen Maruwge

    2008-11-01

    Full Text Available Wessen Maruwge1, Pádraig D’Arcy1, Annika Folin1,2, Slavica Brnjic1, Johan Wejde1, Anthony Davis1, Fredrik Erlandsson3, Jonas Bergh1,2, Bertha Brodin11Department of Oncology and Pathology, Karolinska Institutet, Stockholm, Sweden; 2Radiumhemmet, Karolinska University Hospital, Stockholm, Sweden; 3Bayer Pharmaceutical Corporation, SwedenAbstract: The growth of many soft tissue sarcomas is dependent on aberrant growth factor signaling, which promotes their proliferation and motility. With this in mind, we evaluated the effect of sorafenib, a receptor tyrosine kinase inhibitor, on cell growth and apoptosis in sarcoma cell lines of various histological subtypes. We found that sorafenib effectively inhibited cell proliferation in rhabdomyosarcoma, synovial sarcoma and Ewing’s sarcoma with IC50 values <5 µM. Sorafenib effectively induced growth arrest in rhabdomyosarcoma cells, which was concurrent with inhibition of Akt and Erk signaling. Studies of ligand-induced phosphorylation of Erk and Akt in rhabdomyosarcoma cells showed that insulin-like growth factor-1 is a potent activator, which can be blocked by treatment with sorafenib. In vivo sorafenib treatment of rhabdomyosarcoma xenografts had a significant inhibitory effect on tumor growth, which was associated with inhibited vascularization and enhanced necrosis in the adjacent tumor stroma. Our results demonstrate that in vitro and in vivo growth of rhabdomyosarcoma can be suppressed by treatment with sorafenib, and suggests the possibilities of using sorafenib as a potential adjuvant therapy for the treatment of rhabdomyosarcoma.Keywords: soft tissue sarcoma, kinase inhibitors, targeted therapy, vascularization

  4. In vitro pharmacological characterization of a novel TRPA1 antagonist and proof of mechanism in a human dental pulp model

    Directory of Open Access Journals (Sweden)

    Nyman E

    2013-01-01

    Full Text Available Eva Nyman,1,* Bo Franzén,1,* Andreas Nolting,1 Göran Klement,1 Gang Liu,1 Maria Nilsson,1 Annika Rosén,2 Charlotta Björk,3 Dirk Weigelt,4 Patrik Wollberg,1 Paul Karila,1 Patrick Raboisson11Neuroscience, Innovative Medicines CNS/Pain, AstraZeneca R&D, Södertälje, Sweden; 2Division of Oral and Maxillofacial Surgery, Karolinska Institute/Karolinska University Hospital, Huddinge, Sweden; 3Clinical TA NS Early Development, 4Medicinal Chemistry, Innovative Medicines CNS/Pain, AstraZeneca R&D, Södertälje, Sweden*These authors contributed equally to this workAbstract: AZ465 is a novel selective transient receptor potential cation channel, member A1 (TRPA1 antagonist identified during a focused drug discovery effort. In vitro, AZ465 fully inhibits activation by zinc, O-chlorobenzylidene malononitrile (CS, or cinnamaldehyde of the human TRPA1 channel heterologously expressed in human embryonic kidney cells. Our data using patch-clamp recordings and mouse/human TRPA1 chimeras suggest that AZ465 binds reversibly in the pore region of the human TRPA1 channel. Finally, in an ex vivo model measuring TRPA1 agonist-stimulated release of neuropeptides from human dental pulp biopsies, AZD465 was able to block 50%–60% of CS-induced calcitonin gene-related peptide release, confirming that AZ465 inhibits the native human TRPA1 channel in neuronal tissue.Keywords: pain, pharmacology, antagonist, chimeric proteins, dental pulp, inflammation, neuropeptide, calcitonin gene-related peptide, CGRP

  5. Fifteen Challenges in Establishing a Multidisciplinary Research Program on eHealth Research in a University Setting: A Case Study.

    Science.gov (United States)

    Grönqvist, Helena; Olsson, Erik Martin Gustaf; Johansson, Birgitta; Held, Claes; Sjöström, Jonas; Lindahl Norberg, Annika; Hovén, Emma; Sanderman, Robbert; van Achterberg, Theo; von Essen, Louise

    2017-05-23

    U-CARE is a multidisciplinary eHealth research program that involves the disciplines of caring science, clinical psychology, health economics, information systems, and medical science. It was set up from scratch in a university setting in 2010, funded by a governmental initiative. While establishing the research program, many challenges were faced. Systematic documentation of experiences from establishing new research environments is scarce. The aim of this paper was to describe the challenges of establishing a publicly funded multidisciplinary eHealth research environment. Researchers involved in developing the research program U-CARE identified challenges in the formal documentation and by reflecting on their experience of developing the program. The authors discussed the content and organization of challenges into themes until consensus was reached. The authors identified 15 major challenges, some general to establishing a new research environment and some specific for multidisciplinary eHealth programs. The challenges were organized into 6 themes: Organization, Communication, Implementation, Legislation, Software development, and Multidisciplinarity. Several challenges were faced during the development of the program and several accomplishments were made. By sharing our experience, we hope to help other research groups embarking on a similar journey to be prepared for some of the challenges they are likely to face on their way. ©Helena Grönqvist, Erik Martin Gustaf Olsson, Birgitta Johansson, Claes Held, Jonas Sjöström, Annika Lindahl Norberg, Emma Hovén, Robbert Sanderman, Theo van Achterberg, Louise von Essen. Originally published in the Journal of Medical Internet Research (http://www.jmir.org), 23.05.2017.

  6. Fetal alcohol-spectrum disorders: identifying at-risk mothers

    Directory of Open Access Journals (Sweden)

    Montag AC

    2016-07-01

    Full Text Available Annika C Montag Department of Pediatrics, Division of Dysmorphology and Teratology, University of California San Diego, San Diego, CA, USA Abstract: Fetal alcohol-spectrum disorders (FASDs are a collection of physical and neuro­behavioral disabilities caused by prenatal exposure to alcohol. To prevent or mitigate the costly effects of FASD, we must identify mothers at risk for having a child with FASD, so that we may reach them with interventions. Identifying mothers at risk is beneficial at all time points, whether prior to pregnancy, during pregnancy, or following the birth of the child. In this review, three approaches to identifying mothers at risk are explored: using characteristics of the mother and her pregnancy, using laboratory biomarkers, and using self-report assessment of alcohol-consumption risk. At present, all approaches have serious limitations. Research is needed to improve the sensitivity and specificity of biomarkers and screening instruments, and to link them to outcomes as opposed to exposure. Universal self-report screening of all women of childbearing potential should ideally be incorporated into routine obstetric and gynecologic care, followed by brief interventions, including education and personalized feedback for all who consume alcohol, and referral to treatment as indicated. Effective biomarkers or combinations of biomarkers may be used during pregnancy and at birth to determine maternal and fetal alcohol exposure. The combination of self-report and biomarker screening may help identify a greater proportion of women at risk for having a child with FASD, allowing them to access information and treatment, and empowering them to make decisions that benefit their children. Keywords: fetal alcohol-spectrum disorder (FASD, alcohol, pregnancy, screening, biomarkers, SBIRT

  7. Secular trends in lipid-lowering treatment and lipid levels after a first acute myocardial infarction

    Directory of Open Access Journals (Sweden)

    Lena Björck

    2008-01-01

    Full Text Available Lena Björck1, Catharina Welin2, Annika Rosengren11Department of medicine, Sahlgrenska University Hospital/Östra, Göteborg, Sweden; 2Institute of Health and Care Sciences, the Sahlgrenska Academy at Göteborg University, Göteborg, SwedenBackground: The beneficial effects of statins were unequivocally demonstrated in the Scandinavian Simvastatin Survival Study (4S in 1994, leading to an increase in the use of lipid-lowering drugs. However, to what extent this translates into serum cholesterol levels in a real-life setting has not been systematically investigated.Objective: To estimate secular trends from 1994 to 2002 in blood lipid levels among unselected younger patients after a first acute myocardial infarction (AMI.Method: Descriptive single centre study using consecutive data collection in 781 patients (aged <65 years hospitalized with a first AMI during the period 1994–2002.Results: From 1994–2002, the use of lipid-lowering drugs increased from 10% to 94% for men and from 23% to 90% for women. In 1994, the mean serum cholesterol levels were 6.53 mmol/l in men and 6.32 mmol/l in women, decreasing to 4.31 mmol/l and 5.13 mmol/l in men and women, respectively, in 2002. Still, only 56% of the men and 35% of the women had total serum cholesterol levels <4.5 mmol/l in 2002.Conclusion: Despite a marked increase in lipid-lowering drug treatment in which there was an increase from about 10% in 1994 to more than 90% in 2002, current target levels of <4.5 mmol/l for serum cholesterol were not achieved in a significant proportion of post-AMI patients.Keywords: lipid levels, lipid-lowering medication, myocardial infarction, risk factors

  8. Birch pollen influence the severity of atopic eczema – prospective clinical cohort pilot study and ex vivo penetration study

    Directory of Open Access Journals (Sweden)

    Fölster-Holst R

    2015-10-01

    Full Text Available Regina Fölster-Holst,1 Jagoda Galecka,1 Sigo Weißmantel,1 Ute Dickschat,2 Frank Rippke,3 Kerstin Bohnsack,3 Thomas Werfel,4 Katja Wichmann,4 Matthias Buchner,1 Thomas Schwarz,1 Annika Vogt,5 Jürgen Lademann,5 Martina C Meinke5 1Department of Dermatology, Venerology and Allergy, University of Kiel, 2Wörth, 3Beiersdorf AG, Hamburg, 4Department of Dermatology, Venerology and Allergy, Division of Immunodermatology and Allergy Research, Hannover Medical School, Hannover, 5Department of Dermatology, Venerology and Allergology, Charité – Universitätsmedizin Berlin, Berlin, Germany Abstract: There is little clinical evidence for a correlation between the severity of atopic eczema (AE and pollen exposition. To obtain more data, we performed a clinical cohort pilot study about the influence of pollen on AE between sensitized and nonsensitized subjects and an experimental study addressing the cutaneous penetration of pollen into the skin. Fifty-five patients were monitored during birch pollen season. To study the cutaneous penetration, grass pollen allergens were applied on excised skin and the uptake in CD1c-expressing dendritic cells was investigated. The correlation between environmental pollen load and severity of the Scoring Atopic Dermatitis (SCORAD score and pruritus was observed, regardless of the status of sensitization. The sensitized group recovered significantly worse after the birch pollen season. Remarkably higher amounts of pollen allergens taken up by CD1c cells were detected in epidermal cells derived from skin explants with a disturbed epidermal barrier. These findings suggest an exacerbating role of pollen in AE utilizing the epidermal route. Keywords: aeroallergens, atopic eczema, seasonality, skin antigen-presenting cells, skin barrier penetration

  9. The sub-nucleolar localization of PHF6 defines its role in rDNA transcription and early processing events

    Science.gov (United States)

    Todd, Matthew A M; Huh, Michael S; Picketts, David J

    2016-01-01

    Ribosomal RNA synthesis occurs in the nucleolus and is a tightly regulated process that is targeted in some developmental diseases and hyperactivated in multiple cancers. Subcellular localization and immunoprecipitation coupled mass spectrometry demonstrated that a proportion of plant homeodomain (PHD) finger protein 6 (PHF6) protein is localized within the nucleolus and interacts with proteins involved in ribosomal processing. PHF6 sequence variants cause Börjeson–Forssman–Lehmann syndrome (BFLS, MIM#301900) and are also associated with a female-specific phenotype overlapping with Coffin–Siris syndrome (MIM#135900), T-cell acute lymphoblastic leukemia (MIM#613065), and acute myeloid leukemia (MIM#601626); however, very little is known about its cellular function, including its nucleolar role. HEK 293T cells were treated with RNase A, DNase I, actinomycin D, or 5,6-dichloro-β-D-ribofuranosylbenzimadole, followed by immunocytochemistry to determine PHF6 sub-nucleolar localization. We observed RNA-dependent localization of PHF6 to the sub-nucleolar fibrillar center (FC) and dense fibrillar component (DFC), at whose interface rRNA transcription occurs. Subsequent ChIP-qPCR analysis revealed strong enrichment of PHF6 across the entire rDNA-coding sequence but not along the intergenic spacer (IGS) region. When rRNA levels were quantified in a PHF6 gain-of-function model, we observed an overall decrease in rRNA transcription, accompanied by a modest increase in repressive promoter-associated RNA (pRNA) and a significant increase in the expression levels of the non-coding IGS36RNA and IGS39RNA transcripts. Collectively, our results demonstrate a role for PHF6 in carefully mediating the overall levels of ribosome biogenesis within a cell. PMID:27165002

  10. RNA versatility, flexibility, and thermostability for practice in RNA nanotechnology and biomedical applications.

    Science.gov (United States)

    Haque, Farzin; Pi, Fengmei; Zhao, Zhengyi; Gu, Shanqing; Hu, Haibo; Yu, Hang; Guo, Peixuan

    2018-01-01

    In recent years, RNA has attracted widespread attention as a unique biomaterial with distinct biophysical properties for designing sophisticated architectures in the nanometer scale. RNA is much more versatile in structure and function with higher thermodynamic stability compared to its nucleic acid counterpart DNA. Larger RNA molecules can be viewed as a modular structure built from a combination of many 'Lego' building blocks connected via different linker sequences. By exploiting the diversity of RNA motifs and flexibility of structure, varieties of RNA architectures can be fabricated with precise control of shape, size, and stoichiometry. Many structural motifs have been discovered and characterized over the years and the crystal structures of many of these motifs are available for nanoparticle construction. For example, using the flexibility and versatility of RNA structure, RNA triangles, squares, pentagons, and hexagons can be constructed from phi29 pRNA three-way-junction (3WJ) building block. This review will focus on 2D RNA triangles, squares, and hexamers; 3D and 4D structures built from basic RNA building blocks; and their prospective applications in vivo as imaging or therapeutic agents via specific delivery and targeting. Methods for intracellular cloning and expression of RNA molecules and the in vivo assembly of RNA nanoparticles will also be reviewed. WIREs RNA 2018, 9:e1452. doi: 10.1002/wrna.1452 This article is categorized under: RNA Methods > RNA Nanotechnology RNA Structure and Dynamics > RNA Structure, Dynamics and Chemistry RNA in Disease and Development > RNA in Disease Regulatory RNAs/RNAi/Riboswitches > Regulatory RNAs. © 2017 Wiley Periodicals, Inc.

  11. A phase I trial of vorinostat and alvocidib in patients with relapsed, refractory, or poor prognosis acute leukemia, or refractory anemia with excess blasts-2.

    Science.gov (United States)

    Holkova, Beata; Supko, Jeffrey G; Ames, Matthew M; Reid, Joel M; Shapiro, Geoffrey I; Perkins, Edward Brent; Ramakrishnan, Viswanathan; Tombes, Mary Beth; Honeycutt, Connie; McGovern, Renee M; Kmieciak, Maciej; Shrader, Ellen; Wellons, Martha D; Sankala, Heidi; Doyle, Austin; Wright, John; Roberts, John D; Grant, Steven

    2013-04-01

    This phase I study was conducted to identify the maximum-tolerated dose (MTD) of alvocidib when combined with vorinostat in patients with relapsed, refractory, or poor prognosis acute leukemia, or refractory anemia with excess blasts-2. Secondary objectives included investigating the pharmacokinetic and pharmacodynamic effects of the combination. Patients received vorinostat (200 mg orally, three times a day, for 14 days) on a 21-day cycle, combined with 2 different alvocidib administration schedules: a 1-hour intravenous infusion, daily × 5; or a 30-minute loading infusion followed by a 4-hour maintenance infusion, weekly × 2. The alvocidib dose was escalated using a standard 3+3 design. Twenty-eight patients were enrolled and treated. The alvocidib MTD was 20 mg/m(2) (30-minute loading infusion) followed by 20 mg/m(2) (4-hour maintenance infusion) on days one and eight, in combination with vorinostat. The most frequently encountered toxicities were cytopenias, fatigue, hyperglycemia, hypokalemia, hypophosphatemia, and QT prolongation. Dose-limiting toxicities (DLT) were cardiac arrhythmia-atrial fibrillation and QT prolongation. No objective responses were achieved although 13 of 26 evaluable patients exhibited stable disease. Alvocidib seemed to alter vorinostat pharmacokinetics, whereas alvocidib pharmacokinetics were unaffected by vorinostat. Ex vivo exposure of leukemia cells to plasma obtained from patients after alvocidib treatment blocked vorinostat-mediated p21(CIP1) induction and downregulated Mcl-1 and p-RNA Pol II for some specimens, although parallel in vivo bone marrow responses were infrequent. Alvocidib combined with vorinostat is well tolerated. Although disease stabilization occurred in some heavily pretreated patients, objective responses were not obtained with these schedules. ©2013 AACR.

  12. The predictive value of somatic and cognitive depressive symptoms for cytokine changes in patients with major depression

    Directory of Open Access Journals (Sweden)

    Dannehl K

    2014-06-01

    Full Text Available Katharina Dannehl,1 Winfried Rief,1 Markus J Schwarz,2 Annika Hennings,1 Sabine Riemer,1 Verena Selberdinger,3 Theresa Stapf,3 Frank Euteneuer11Division of Clinical Psychology and Psychotherapy, Philipps Universität Marburg, Marburg, Germany; 2Institute for Laboratory Medicine, Ludwig-Maximilian Universität, Munich, Germany; 3Department of Psychiatry, Ludwig-Maximilian Universität, Munich, GermanyContext: Elevated concentrations of proinflammatory cytokines have been hypothesized as an important factor in the pathophysiology of depression. Depression itself is considered to be a heterogeneous disorder. Current findings suggest that “cognitive” and “somatic” symptom dimensions are related to immune function in different ways. So far, little research has been done on the longitudinal aspects of inflammation in patients with major depression, especially with respect to different symptom dimensions of depression. Therefore, we investigated which aspects of depression may predict changes in tumor necrosis factor-alpha (TNF-alpha and interleukin (IL-6 over 4 weeks. Methods: Forty-one patients with major depression diagnosed according to the Diagnostic and Statistical Manual of Mental Disorders, fourth edition (DSM-IV, and 45 healthy controls were enrolled. Serum measurements of TNF-alpha and IL-6 were conducted at baseline and 4 weeks later. Psychometric measures included the assessment of cognitive-affective depressive symptoms and somatic symptoms during the last 7 days as well as somatic symptoms during the last 2 years. Results: Patients with depression showed increased levels of TNF-alpha (P<0.05 compared to healthy controls. Hierarchical regression analyses indicated that neither depressive nor somatic symptoms predict changes in proinflammatory cytokines in the whole sample of depressed patients. Moderation analyses and subsequent sex-stratified regression analyses indicated that higher somatoform symptoms during the last 2 years

  13. Autologous serum improves bone formation in a primary stable silica-embedded nanohydroxyapatite bone substitute in combination with mesenchymal stem cells and rhBMP-2 in the sheep model

    Directory of Open Access Journals (Sweden)

    Boos AM

    2014-11-01

    Full Text Available Anja M Boos,1,* Annika Weigand,1,* Gloria Deschler,1 Thomas Gerber,2 Andreas Arkudas,1 Ulrich Kneser,1 Raymund E Horch,1 Justus P Beier11Department of Plastic and Hand Surgery, University Hospital of Erlangen, Friedrich-Alexander-University of Erlangen-Nürnberg FAU, Erlangen, 2Institute of Physics, University of Rostock, Rostock, Germany *These authors contributed equally to this work Abstract: New therapeutic strategies are required for critical size bone defects, because the gold standard of transplanting autologous bone from an unharmed area of the body often leads to several severe side effects and disadvantages for the patient. For years, tissue engineering approaches have been seeking a stable, axially vascularized transplantable bone replacement suitable for transplantation into the recipient bed with pre-existing insufficient conditions. For this reason, the arteriovenous loop model was developed and various bone substitutes have been vascularized. However, it has not been possible thus far to engineer a primary stable and axially vascularized transplantable bone substitute. For that purpose, a primary stable silica-embedded nanohydroxyapatite (HA bone substitute in combination with blood, bone marrow, expanded, or directly retransplanted mesenchymal stem cells, recombinant human bone morphogenetic protein 2 (rhBMP-2, and different carrier materials (fibrin, cell culture medium, autologous serum was tested subcutaneously for 4 or 12 weeks in the sheep model. Autologous serum lead to an early matrix change during degradation of the bone substitute and formation of new bone tissue. The best results were achieved in the group combining mesenchymal stem cells expanded with 60 µg/mL rhBMP-2 in autologous serum. Better ingrowth of fibrovascular tissue could be detected in the autologous serum group compared with the control (fibrin. Osteoclastic activity indicating an active bone remodeling process was observed after 4 weeks, particularly

  14. Occupational exposure to vapor, gas, dust, or fumes and chronic airflow limitation, COPD, and emphysema: the Swedish CArdioPulmonary BioImage Study (SCAPIS pilot

    Directory of Open Access Journals (Sweden)

    Torén K

    2017-11-01

    Full Text Available Kjell Torén,1 Jenny Vikgren,2 Anna-Carin Olin,1 Annika Rosengren,3 Göran Bergström,3 John Brandberg2 1Section of Occupational and Environmental Medicine, Institute of Medicine, Sahlgrenska Academy, 2Department of Radiology, Institute of Clinical Sciences, 3Department of Molecular and Clinical Medicine, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden Background: The aim of this study was to estimate the occupational burden of airflow limitation, chronic airflow limitation, COPD, and emphysema.Materials and methods: Subjects aged 50–64 years (n=1,050 were investigated with forced expiratory volume in 1 s (FEV1 and forced vital capacity (FVC. Airflow limitation was defined as FEV1/FVC <0.7 before bronchodilation. Chronic airflow limitation was defined after bronchodilation either according to the Global Initiative for Chronic Obstructive Lung Disease (GOLD as FEV1/FVC <0.7 or according to the lower limit of normal (LLN approach as FEV1/FVC < LLN. COPD was defined as chronic airflow limitation (GOLD in combination with dyspnea, wheezing, or chronic bronchitis. Emphysema was classified according to findings from computed tomography of the lungs. Occupational exposure was defined as self-reported occupational exposure to vapor, gas, dust, or fumes (VGDF. Odds ratios (OR were calculated in models adjusted for age, gender, and smoking; population-attributable fractions and 95% CI were also calculated.Results: There were significant associations between occupational exposure to VGDF and COPD (OR 2.7, 95% CI 1.4–51, airflow limitation (OR 1.8, 95% CI 1.3–2.5, and emphysema (OR 1.8, 95% CI 1.1–3.1. The associations between occupational exposure to VGDF and chronic airflow limitation were weaker, and for the OR, the CIs included unity. The population-attributable fraction for occupational exposure to VGDF was 0.37 (95% CI 0.23–0.47 for COPD and 0.23 (95% CI 0.05–0.35 for emphysema.Conclusion: The

  15. New Nordic Exceptionalism: Jeuno JE Kim and Ewa Einhorn's The United Nations of Norden and other realist utopias

    Directory of Open Access Journals (Sweden)

    Mathias Danbolt

    2016-11-01

    Full Text Available At the 2009 Nordic Culture Forum summit in Berlin that centered on the profiling and branding of the Nordic region in a globalized world, one presenter stood out from the crowd. The lobbyist Annika Sigurdardottir delivered a speech that called for the establishment of “The United Nations of Norden”: A Nordic union that would gather the nations and restore Norden's role as the “moral superpower of the world.” Sigurdardottir's presentation generated such a heated debate that the organizers had to intervene and reveal that the speech was a performance made by the artists Jeuno JE Kim and Ewa Einhorn. This article takes Kim and Einhorn's intervention as a starting point for a critical discussion of the history and politics of Nordic image-building. The article suggests that the reason Kim and Einhorn's speech passed as a serious proposal was due to its meticulous mimicking of two discursive formations that have been central to the debates on the branding of Nordicity over the last decades: on the one hand, the discourse of “Nordic exceptionalism,” that since the 1960s has been central to the promotion of a Nordic political, socio-economic, and internationalist “third way” model, and, on the other hand, the discourse on the “New Nordic,” that emerged out of the New Nordic Food-movement in the early 2000s, and which has given art and culture a privileged role in the international re-fashioning of the Nordic brand. Through an analysis of Kim and Einhorn's United Nations of Norden (UNN-performance, the article examines the historical development and ideological underpinnings of the image of Nordic unity at play in the discourses of Nordic exceptionalism and the New Nordic. By focusing on how the UNN-project puts pressure on the role of utopian imaginaries in the construction of Nordic self-images, the article describes the emergence of a discursive framework of New Nordic Exceptionalism.

  16. Validity of physician-diagnosed COPD in relation to spirometric definitions of COPD in a general population aged 50–64 years – the SCAPIS pilot study

    Directory of Open Access Journals (Sweden)

    Torén K

    2017-08-01

    Full Text Available Kjell Torén,1,2 Nicola Murgia,1,2 Anna-Carin Olin,1 Jan Hedner,3 John Brandberg,4 Annika Rosengren,5 Göran Bergström51Section of Occupational and Environmental Medicine, Sahlgrenska Academy, University of Gothenburg, Sweden; 2Section of Occupational Medicine, Respiratory Diseases and Toxicology, University of Perugia, Italy; 3Department of Medicine/Lung Medicine, Sahlgrenska Academy, University of Gothenburg, 4Department of Radiology, Institute of Clinical Sciences, Sahlgrenska Academy, University of Gothenburg, 5Department of Molecular and Clinical Medicine, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Sweden Background: In epidemiological studies, items about physician-diagnosed COPD are often used. There is a lack of validation and standardization of these items.Materials and methods: In a general population-based study, 1,050 subjects completed a questionnaire and performed spirometry, including forced expiratory volume in 1 second (FEV1 and forced vital capacity (FVC after inhalation of 400 µg of salbutamol. COPD was defined as the ratio of FEV1/FVC <0.7 after bronchodilation. Physician-diagnosed COPD was defined as an affirmative answer to the single item: “Have you ever had COPD diagnosed by a physician?”, physician-diagnosed COPD/emphysema as an affirmative answer to any of the two single items; “Have you ever had COPD diagnosed by a physician?” or “Have you ever been told by a physician that you have emphysema?”, physician-diagnosed chronic bronchitis as an affirmative answer to; “Have you ever been told by a physician that you have chronic bronchitis?” and physician-diagnosed COPD, emphysema or chronic bronchitis was defined as an affirmative answer to either of the three items above.Results: For the single item about physician-diagnosed COPD, the sensitivity was around 0.11 and the specificity was almost 0.99 in relation to COPD. The sensitivity of the combined items about COPD

  17. Vital capacity and COPD: the Swedish CArdioPulmonary bioImage Study (SCAPIS

    Directory of Open Access Journals (Sweden)

    Torén K

    2016-05-01

    Full Text Available Kjell Torén,1 Anna-Carin Olin,1 Anne Lindberg,2 Jenny Vikgren,3 Linus Schiöler,1 John Brandberg,3 Åse Johnsson,3 Gunnar Engström,4 H Lennart Persson,5 Magnus Sköld,6 Jan Hedner,7 Eva Lindberg,8 Andrei Malinovschi,8 Eeva Piitulainen,9 Per Wollmer,9 Annika Rosengren,10 Christer Janson,8 Anders Blomberg,2 Göran Bergström10 1Section of Occupational and Environmental Medicine, Department of Molecular and Clinical Medicine, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, 2Department of Public Health and Clinical Medicine, Division of Medicine/Respiratory Medicine, Umeå University, Umeå, 3Department of Radiology, Institute of Clinical Sciences, Sahlgrenska Academy, University of Gothenburg, Gothenburg, 4Department of Clinical Science, Malmö, Lund University, Lund, 5Department of Respiratory Medicine and Department of Medicine and Health Sciences, Linköping University, Linköping, 6Respiratory Medicine Unit, Department of Medicine Solna, Center for Molecular Medicine, Karolinska Institutet, Stockholm, 7Department of Internal Medicine/Lung Medicine, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, 8Department of Medical Sciences, Clinical Physiology and Respiratory, Allergy and Sleep Research, Uppsala University, Uppsala, 9Department of Translational Medicine, Lund University, Malmö, 10Department of Molecular and Clinical Medicine, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden Background: Spirometric diagnosis of chronic obstructive pulmonary disease (COPD is based on the ratio of forced expiratory volume in 1 second (FEV1/vital capacity (VC, either as a fixed value <0.7 or below the lower limit of normal (LLN. Forced vital capacity (FVC is a proxy for VC. The first aim was to compare the use of FVC and VC, assessed as the highest value of FVC or slow vital capacity (SVC, when assessing the FEV1/VC ratio in a general population