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Sample records for ankara modificado mva

  1. Vectores recombinantes basados en el virus Vaccinia modificado de Ankara (MVA) como vacunas contra la leishmaniasis

    OpenAIRE

    Pérez Jiménez, Eva; Larraga, Vicente; Esteban, Mariano

    2005-01-01

    Vectores recombinantes basados en el virus vaccinia modificado de Ankara (MVA) como vacunas contra la leishmaniasis. Los vectores de la invención contienen secuencias codificantes de la proteína LACK, preferentemente insertadas en el locus de hemaglutinina del virus y bajo el control de un promotor que permite su expresión a lo largo del ciclo de infección del virus. Son vectores seguros, estables, que dan lugar a una potente respuesta inmune que confiere protección frente a la leishmaniasis,...

  2. Vectores recombinantes basados en el virus modificado de ankara (MVA) como vacunas preventivas y terapéuticas contra el SIDA

    OpenAIRE

    Jonathan L Heeney; Mooij, Petra; Nájera García, José Luis; Jiménez, Victoria; Esteban, Mariano; Gómez, Carmen E.

    2005-01-01

    Vectores Recombinantes basados en el Virus Modificado de Ankara (MVA) como Vacunas Preventivas y Terapéuticas contra el SIDA. Losvirus recombinantes de la invención contienen secuencias que se encuentran insertadas en el mismo sitio de inserción del MVA y permiten la expresión simultánea de varios antígenos, una proteína Env del VIH-I consistente en una proteína gpl20 carente de secuencias correspondientes a la proteína gp41, y una proteína quiméricade fusión de Gag, Pol y Nef. Son virus esta...

  3. Introduction of the Six Major Genomic Deletions of Modified Vaccinia Virus Ankara (MVA) into the Parental Vaccinia Virus Is Not Sufficient To Reproduce an MVA-Like Phenotype in Cell Culture and in Mice▿

    OpenAIRE

    Meisinger-Henschel, Christine; Späth, Michaela; Lukassen, Susanne; Wolferstätter, Michael; Kachelriess, Heike; Baur, Karen; Dirmeier, Ulrike; Wagner, Markus; Chaplin, Paul; Suter, Mark; Hausmann, Jürgen

    2010-01-01

    Modified vaccinia virus Ankara (MVA) has a highly restricted host range in cell culture and is apathogenic in vivo. MVA was derived from the parental chorioallantois vaccinia virus Ankara (CVA) by more than 570 passages in chicken embryo fibroblast (CEF) cells. During CEF cell passaging, six major deletions comprising 24,668 nucleotides occurred in the CVA genome. We have cloned both the MVA and the parental CVA genome as bacterial artificial chromosomes (BACs) and have sequentially introduce...

  4. Recombination-mediated genetic engineering of a bacterial artificial chromosome clone of modified vaccinia virus Ankara (MVA)

    DEFF Research Database (Denmark)

    Cottingham, Matthew G; Andersen, Rikke F; Spencer, Alexandra J;

    2008-01-01

    infectious virus using a Fowlpox virus helper to supply transcriptional machinery. We apply here a similar approach to the attenuated strain Modified Vaccinia virus Ankara (MVA), now widely used as a safe non-replicating recombinant vaccine vector in mammals, including humans. Four apparently full...... using GalK counterselection to insert an antigen expression cassette lacking a tandem marker gene into the traditional thymidine kinase locus of MVA-BAC. MVA continues to feature prominently in clinical trials of recombinant vaccines against diseases such as HIV-AIDS, malaria and tuberculosis. Here we...

  5. Cross-protective immunity against multiple influenza virus subtypes by a novel modified vaccinia Ankara (MVA) vectored vaccine in mice.

    Science.gov (United States)

    Brewoo, Joseph N; Powell, Tim D; Jones, Jeremy C; Gundlach, Nancy A; Young, Ginger R; Chu, Haiyan; Das, Subash C; Partidos, Charalambos D; Stinchcomb, Dan T; Osorio, Jorge E

    2013-04-01

    Development of an influenza vaccine that provides cross-protective immunity remains a challenge. Candidate vaccines based on a recombinant modified vaccinia Ankara (MVA) viral vector expressing antigens from influenza (MVA/Flu) viruses were constructed. A vaccine candidate, designated MVA/HA1/C13L/NP, that expresses the hemagglutinin from pandemic H1N1 (A/California/04/09) and the nucleoprotein (NP) from highly pathogenic H5N1 (A/Vietnam/1203/04) fused to a secretory signal sequence from vaccinia virus was highly protective. The vaccine elicited strong antibody titers to homologous H1N1 viruses while cross-reactive antibodies to heterologous viruses were not detectable. In mice, this MVA/HA1/C13L/NP vaccine conferred complete protection against lethal challenge with A/Vietnam/1203/04 (H5N1), A/Norway/3487-2/09 (pandemic H1N1) or A/Influenza/Puerto Rico/8/34 (seasonal H1N1) and partial protection (57.1%) against challenge with seasonal H3N2 virus (A/Aichi/68). The protective efficacy of the vaccine was not affected by pre-existing immunity to vaccinia. Our findings highlight MVA as suitable vector to express multiple influenza antigens that could afford broad cross-protective immunity against multiple subtypes of influenza virus. PMID:23376279

  6. Induction of antibody responses to African horse sickness virus (AHSV in ponies after vaccination with recombinant modified vaccinia Ankara (MVA.

    Directory of Open Access Journals (Sweden)

    Rachael Chiam

    Full Text Available BACKGROUND: African horse sickness virus (AHSV causes a non-contagious, infectious disease in equids, with mortality rates that can exceed 90% in susceptible horse populations. AHSV vaccines play a crucial role in the control of the disease; however, there are concerns over the use of polyvalent live attenuated vaccines particularly in areas where AHSV is not endemic. Therefore, it is important to consider alternative approaches for AHSV vaccine development. We have carried out a pilot study to investigate the ability of recombinant modified vaccinia Ankara (MVA vaccines expressing VP2, VP7 or NS3 genes of AHSV to stimulate immune responses against AHSV antigens in the horse. METHODOLOGY/PRINCIPAL FINDINGS: VP2, VP7 and NS3 genes from AHSV-4/Madrid87 were cloned into the vaccinia transfer vector pSC11 and recombinant MVA viruses generated. Antigen expression or transcription of the AHSV genes from cells infected with the recombinant viruses was confirmed. Pairs of ponies were vaccinated with MVAVP2, MVAVP7 or MVANS3 and both MVA vector and AHSV antigen-specific antibody responses were analysed. Vaccination with MVAVP2 induced a strong AHSV neutralising antibody response (VN titre up to a value of 2. MVAVP7 also induced AHSV antigen-specific responses, detected by western blotting. NS3 specific antibody responses were not detected. CONCLUSIONS: This pilot study demonstrates the immunogenicity of recombinant MVA vectored AHSV vaccines, in particular MVAVP2, and indicates that further work to investigate whether these vaccines would confer protection from lethal AHSV challenge in the horse is justifiable.

  7. Construction of Recombinant Modified Vaccinia Ankara (MVA) Expressing Hepatitis B Virus Surface Antigen

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    The T lymphocyte response has been shown to be the determinant in the clearance of many viral infections.Hence, therapeutic vaccine candidates against HBV are designed to enhance this response of the immune system.Vaccinia virus vector-based vaccines have been proposed as excellent candidates to elicit long-term and strong T lymphocyte mediated immune responses. In this study, the recombinant MVA expressing HBV surface antigen has been constructed, which can elicit a potent T cell mediated response. The ELISA results for the surface protein in the medium of the recombinant MVA, strongly indicate that the recombinant virus has been successfully obtained.

  8. Introduction of the Six Major Genomic Deletions of Modified Vaccinia Virus Ankara (MVA) into the Parental Vaccinia Virus Is Not Sufficient To Reproduce an MVA-Like Phenotype in Cell Culture and in Mice▿

    Science.gov (United States)

    Meisinger-Henschel, Christine; Späth, Michaela; Lukassen, Susanne; Wolferstätter, Michael; Kachelriess, Heike; Baur, Karen; Dirmeier, Ulrike; Wagner, Markus; Chaplin, Paul; Suter, Mark; Hausmann, Jürgen

    2010-01-01

    Modified vaccinia virus Ankara (MVA) has a highly restricted host range in cell culture and is apathogenic in vivo. MVA was derived from the parental chorioallantois vaccinia virus Ankara (CVA) by more than 570 passages in chicken embryo fibroblast (CEF) cells. During CEF cell passaging, six major deletions comprising 24,668 nucleotides occurred in the CVA genome. We have cloned both the MVA and the parental CVA genome as bacterial artificial chromosomes (BACs) and have sequentially introduced the six major MVA deletions into the cloned CVA genome. Reconstituted mutant CVA viruses containing up to six major MVA deletions showed no detectable replication restriction in 12 of 14 mammalian cell lines tested; the exceptions were rabbit cell lines RK13 and SIRC. In mice, CVA mutants with up to three deletions showed slightly enhanced virulence, suggesting that gene deletion in replicating vaccinia virus (VACV) can result in gain of fitness in vivo. CVA mutants containing five or all six deletions were still pathogenic, with a moderate degree of attenuation. Deletion V was mainly responsible for the attenuated phenotype of these mutants. In conclusion, loss or truncation of all 31 open reading frames in the six major deletions is not sufficient to reproduce the specific MVA phenotype of strong attenuation and highly restricted host range. Mutations in viral genes outside or in association with the six major deletions appear to contribute significantly to this phenotype. Host range restriction and avirulence of MVA are most likely a cooperative effect of gene deletions and mutations involving the major deletions. PMID:20668072

  9. Introduction of the six major genomic deletions of modified vaccinia virus Ankara (MVA) into the parental vaccinia virus is not sufficient to reproduce an MVA-like phenotype in cell culture and in mice.

    Science.gov (United States)

    Meisinger-Henschel, Christine; Späth, Michaela; Lukassen, Susanne; Wolferstätter, Michael; Kachelriess, Heike; Baur, Karen; Dirmeier, Ulrike; Wagner, Markus; Chaplin, Paul; Suter, Mark; Hausmann, Jürgen

    2010-10-01

    Modified vaccinia virus Ankara (MVA) has a highly restricted host range in cell culture and is apathogenic in vivo. MVA was derived from the parental chorioallantois vaccinia virus Ankara (CVA) by more than 570 passages in chicken embryo fibroblast (CEF) cells. During CEF cell passaging, six major deletions comprising 24,668 nucleotides occurred in the CVA genome. We have cloned both the MVA and the parental CVA genome as bacterial artificial chromosomes (BACs) and have sequentially introduced the six major MVA deletions into the cloned CVA genome. Reconstituted mutant CVA viruses containing up to six major MVA deletions showed no detectable replication restriction in 12 of 14 mammalian cell lines tested; the exceptions were rabbit cell lines RK13 and SIRC. In mice, CVA mutants with up to three deletions showed slightly enhanced virulence, suggesting that gene deletion in replicating vaccinia virus (VACV) can result in gain of fitness in vivo. CVA mutants containing five or all six deletions were still pathogenic, with a moderate degree of attenuation. Deletion V was mainly responsible for the attenuated phenotype of these mutants. In conclusion, loss or truncation of all 31 open reading frames in the six major deletions is not sufficient to reproduce the specific MVA phenotype of strong attenuation and highly restricted host range. Mutations in viral genes outside or in association with the six major deletions appear to contribute significantly to this phenotype. Host range restriction and avirulence of MVA are most likely a cooperative effect of gene deletions and mutations involving the major deletions. PMID:20668072

  10. A Modified Vaccinia Ankara Virus (MVA) Vaccine Expressing African Horse Sickness Virus (AHSV) VP2 Protects Against AHSV Challenge in an IFNAR −/− Mouse Model

    Science.gov (United States)

    Castillo-Olivares, Javier; Calvo-Pinilla, Eva; Casanova, Isabel; Bachanek-Bankowska, Katarzyna; Chiam, Rachael; Maan, Sushila; Nieto, Jose Maria; Ortego, Javier; Mertens, Peter Paul Clement

    2011-01-01

    African horse sickness (AHS) is a lethal viral disease of equids, which is transmitted by Culicoides midges that become infected after biting a viraemic host. The use of live attenuated vaccines has been vital for the control of this disease in endemic regions. However, there are safety concerns over their use in non-endemic countries. Research efforts over the last two decades have therefore focused on developing alternative vaccines based on recombinant baculovirus or live viral vectors expressing structural components of the AHS virion. However, ethical and financial considerations, relating to the use of infected horses in high biosecurity installations, have made progress very slow. We have therefore assessed the potential of an experimental mouse-model for AHSV infection for vaccine and immunology research. We initially characterised AHSV infection in this model, then tested the protective efficacy of a recombinant vaccine based on modified vaccinia Ankara expressing AHS-4 VP2 (MVA-VP2). PMID:21298069

  11. Percutaneous Vaccination as an Effective Method of Delivery of MVA and MVA-Vectored Vaccines.

    Directory of Open Access Journals (Sweden)

    Clement A Meseda

    Full Text Available The robustness of immune responses to an antigen could be dictated by the route of vaccine inoculation. Traditional smallpox vaccines, essentially vaccinia virus strains, that were used in the eradication of smallpox were administered by percutaneous inoculation (skin scarification. The modified vaccinia virus Ankara is licensed as a smallpox vaccine in Europe and Canada and currently undergoing clinical development in the United States. MVA is also being investigated as a vector for the delivery of heterologous genes for prophylactic or therapeutic immunization. Since MVA is replication-deficient, MVA and MVA-vectored vaccines are often inoculated through the intramuscular, intradermal or subcutaneous routes. Vaccine inoculation via the intramuscular, intradermal or subcutaneous routes requires the use of injection needles, and an estimated 10 to 20% of the population of the United States has needle phobia. Following an observation in our laboratory that a replication-deficient recombinant vaccinia virus derived from the New York City Board of Health strain elicited protective immune responses in a mouse model upon inoculation by tail scarification, we investigated whether MVA and MVA recombinants can elicit protective responses following percutaneous administration in mouse models. Our data suggest that MVA administered by percutaneous inoculation, elicited vaccinia-specific antibody responses, and protected mice from lethal vaccinia virus challenge, at levels comparable to or better than subcutaneous or intramuscular inoculation. High titers of specific neutralizing antibodies were elicited in mice inoculated with a recombinant MVA expressing the herpes simplex type 2 glycoprotein D after scarification. Similarly, a recombinant MVA expressing the hemagglutinin of attenuated influenza virus rgA/Viet Nam/1203/2004 (H5N1 elicited protective immune responses when administered at low doses by scarification. Taken together, our data suggest that

  12. Recombinant modified vaccinia virus Ankara-based malaria vaccines.

    Science.gov (United States)

    Sebastian, Sarah; Gilbert, Sarah C

    2016-01-01

    A safe and effective malaria vaccine is a crucial part of the roadmap to malaria elimination/eradication by the year 2050. Viral-vectored vaccines based on adenoviruses and modified vaccinia virus Ankara (MVA) expressing malaria immunogens are currently being used in heterologous prime-boost regimes in clinical trials for induction of strong antigen-specific T-cell responses and high-titer antibodies. Recombinant MVA is a safe and well-tolerated attenuated vector that has consistently shown significant boosting potential. Advances have been made in large-scale MVA manufacture as high-yield producer cell lines and high-throughput purification processes have recently been developed. This review describes the use of MVA as malaria vaccine vector in both preclinical and clinical studies in the past 5 years. PMID:26511884

  13. Effects of MVA85A vaccine on tuberculosis challenge in animals: systematic review

    OpenAIRE

    Kashangura, Rufaro; Sena, Emily S.; Young, Taryn; Garner, Paul

    2015-01-01

    Background: The existing Bacillus Calmette–Guérin (BCG) vaccination provides partial protection against tuberculosis (TB). The modified vaccinia ankara virus-expressing antigen 85A (MVA85A) aims to boost BCG immunity. We evaluated the animal evidence supporting the testing of MVA85A in humans. Methods: Our protocol included in vivo preclinical studies of the MVA85A booster with BCG compared with BCG alone, followed by a TB challenge. We used standard methods for systematic review of animal st...

  14. Expression and Cellular Immunogenicity of a Transgenic Antigen Driven by Endogenous Poxviral Early Promoters at Their Authentic Loci in MVA

    OpenAIRE

    Toritse Orubu; Naif Khalaf Alharbi; Teresa Lambe; Sarah C Gilbert; Cottingham, Matthew G

    2012-01-01

    CD8(+) T cell responses to vaccinia virus are directed almost exclusively against early gene products. The attenuated strain modified vaccinia virus Ankara (MVA) is under evaluation in clinical trials of new vaccines designed to elicit cellular immune responses against pathogens including Plasmodium spp., M. tuberculosis and HIV-1. All of these recombinant MVAs (rMVA) utilize the well-established method of linking the gene of interest to a cloned poxviral promoter prior to insertion into the ...

  15. Market implementation of the MVA platform for pre-pandemic and pandemic influenza vaccines: A quantitative key opinion leader analysis

    NARCIS (Netherlands)

    B. Ramezanpour (Bahar); E.S. Pronker (Esther); J.H.C.M. Kreijtz (Joost); A.D.M.E. Osterhaus (Albert); H.J.H.M. Claassen (Eric)

    2015-01-01

    textabstractA quantitative method is presented to rank strengths, weaknesses, opportunities, and threats (SWOT) of modified vaccinia virus Ankara (MVA) as a platform for pre-pandemic and pandemic influenza vaccines. Analytic hierarchy process (AHP) was applied to achieve pairwise comparisons among S

  16. Co-expression of Interleukin-15 Enhances the Protective Immune Responses Induced by Immunization with a Murine Malaria MVA-Based Vaccine Encoding the Circumsporozoite Protein.

    Directory of Open Access Journals (Sweden)

    Marcela Parra

    Full Text Available Malaria remains a major global public health problem with an estimated 200 million cases detected in 2012. Although the most advanced candidate malaria vaccine (RTS,S has shown promise in clinical trials, its modest efficacy and durability have created uncertainty about the impact of RTS,S immunization (when used alone on global malaria transmission. Here we describe the development and characterization of a novel modified vaccinia virus Ankara (MVA-based malaria vaccine which co-expresses the Plasmodium yoelii circumsporozoite protein (CSP and IL-15. Vaccination/challenge studies showed that C57BL/6 mice immunized with the MVA-CSP/IL15 vaccine were protected significantly better against a P. yoelii 17XNL sporozoite challenge than either mice immunized with an MVA vaccine expressing only CSP or naïve controls. Importantly, the levels of total anti-CSP IgG were elevated about 100-fold for the MVA-CSP/IL15 immunized group compared to mice immunized with the MVA-CSP construct that does not express IL-15. Among the IgG subtypes, the IL-15 expressing MVA-CSP vaccine induced levels of IgG1 (8 fold and IgG2b (80 fold higher than the MVA-CSP construct. The significantly enhanced humoral responses and protection detected after immunization with the MVA-CSP/IL15 vaccine suggest that this IL-15 expressing MVA construct could be considered in the development of future malaria immunization strategies.

  17. Co-expression of Interleukin-15 Enhances the Protective Immune Responses Induced by Immunization with a Murine Malaria MVA-Based Vaccine Encoding the Circumsporozoite Protein.

    Science.gov (United States)

    Parra, Marcela; Liu, Xia; Derrick, Steven C; Yang, Amy; Molina-Cruz, Alvaro; Barillas-Mury, Carolina; Zheng, Hong; Thao Pham, Phuong; Sedegah, Martha; Belmonte, Arnel; Litilit, Dianne D; Waldmann, Thomas A; Kumar, Sanjai; Morris, Sheldon L; Perera, Liyanage P

    2015-01-01

    Malaria remains a major global public health problem with an estimated 200 million cases detected in 2012. Although the most advanced candidate malaria vaccine (RTS,S) has shown promise in clinical trials, its modest efficacy and durability have created uncertainty about the impact of RTS,S immunization (when used alone) on global malaria transmission. Here we describe the development and characterization of a novel modified vaccinia virus Ankara (MVA)-based malaria vaccine which co-expresses the Plasmodium yoelii circumsporozoite protein (CSP) and IL-15. Vaccination/challenge studies showed that C57BL/6 mice immunized with the MVA-CSP/IL15 vaccine were protected significantly better against a P. yoelii 17XNL sporozoite challenge than either mice immunized with an MVA vaccine expressing only CSP or naïve controls. Importantly, the levels of total anti-CSP IgG were elevated about 100-fold for the MVA-CSP/IL15 immunized group compared to mice immunized with the MVA-CSP construct that does not express IL-15. Among the IgG subtypes, the IL-15 expressing MVA-CSP vaccine induced levels of IgG1 (8 fold) and IgG2b (80 fold) higher than the MVA-CSP construct. The significantly enhanced humoral responses and protection detected after immunization with the MVA-CSP/IL15 vaccine suggest that this IL-15 expressing MVA construct could be considered in the development of future malaria immunization strategies. PMID:26505634

  18. Co-expression of Interleukin-15 Enhances the Protective Immune Responses Induced by Immunization with a Murine Malaria MVA-Based Vaccine Encoding the Circumsporozoite Protein

    Science.gov (United States)

    Parra, Marcela; Liu, Xia; Derrick, Steven C.; Yang, Amy; Molina-Cruz, Alvaro; Barillas-Mury, Carolina; Zheng, Hong; Thao Pham, Phuong; Sedegah, Martha; Belmonte, Arnel; Litilit, Dianne D.; Waldmann, Thomas A.; Kumar, Sanjai; Morris, Sheldon L.; Perera, Liyanage P.

    2015-01-01

    Malaria remains a major global public health problem with an estimated 200 million cases detected in 2012. Although the most advanced candidate malaria vaccine (RTS,S) has shown promise in clinical trials, its modest efficacy and durability have created uncertainty about the impact of RTS,S immunization (when used alone) on global malaria transmission. Here we describe the development and characterization of a novel modified vaccinia virus Ankara (MVA)–based malaria vaccine which co-expresses the Plasmodium yoelii circumsporozoite protein (CSP) and IL-15. Vaccination/challenge studies showed that C57BL/6 mice immunized with the MVA-CSP/IL15 vaccine were protected significantly better against a P. yoelii 17XNL sporozoite challenge than either mice immunized with an MVA vaccine expressing only CSP or naïve controls. Importantly, the levels of total anti-CSP IgG were elevated about 100-fold for the MVA-CSP/IL15 immunized group compared to mice immunized with the MVA-CSP construct that does not express IL-15. Among the IgG subtypes, the IL-15 expressing MVA-CSP vaccine induced levels of IgG1 (8 fold) and IgG2b (80 fold) higher than the MVA-CSP construct. The significantly enhanced humoral responses and protection detected after immunization with the MVA-CSP/IL15 vaccine suggest that this IL-15 expressing MVA construct could be considered in the development of future malaria immunization strategies. PMID:26505634

  19. Immunogenicity of Lyophilized MVA Vaccine for HIV-1 in Mice Model

    Institute of Scientific and Technical Information of China (English)

    ZHANG Yi-zhe; JIANG Chun-lai; YU Xiang-hui; LOU Chao-ping; ZHAO Dong-hai; WU Yong-ge; JIN Ying-hua; LIU Cheng-shan; KONG Wei

    2007-01-01

    Highly attenuated modified vaccinia Ankara(MVA) is sensitive to repeat freeze-thaw cycle and easy to lose activity. In order to make the activity of MVA vaccine remain stable during its manufacturing, storage, and administration, the lyophilization as a good option could be resorted to; through screening, the right stabilizer composition and its production procedure were obtained. The final moisture content of freezing-dried recombinant MVA-HIV vaccine was lower than 3%. It can be reconstituted quickly and shows regular physical appearance and stable potency. In vivo functional experiment, mice were divided randomly into the liquid vaccination group, the lyophilized vaccination group, and the control group. Having been DNA vaccine priming, the mice were boosted with a dose of 107 pfu MVA-HIV vaccine, which produced indistinguishable antibody titer and cytotoxic T-lymphocyte(CTL) level compared with those of liquid vaccination group(P>0.05). These results demonstrate that lyophilized MVA vaccine can induce high immunogenicity in mice.

  20. A novel naturally occurring tandem promoter in modified vaccinia virus ankara drives very early gene expression and potent immune responses.

    Science.gov (United States)

    Wennier, Sonia T; Brinkmann, Kay; Steinhäußer, Charlotte; Mayländer, Nicole; Mnich, Claudia; Wielert, Ursula; Dirmeier, Ulrike; Hausmann, Jürgen; Chaplin, Paul; Steigerwald, Robin

    2013-01-01

    Modified vaccinia virus Ankara (MVA) has been shown to be suitable for the generation of experimental vaccines against cancer and infectious diseases, eliciting strong humoral and cellular immune responses. In viral vectored vaccines, strong recombinant antigen expression and timing of expression influence the quantity and quality of the immune response. Screening of synthetic and native poxvirus promoters for strong protein expression in vitro and potent immune responses in vivo led to the identification of the MVA13.5L promoter, a unique and novel naturally occurring tandem promoter in MVA composed of two 44 nucleotide long repeated motifs, each containing an early promoter element. The MVA13.5L gene is highly conserved across orthopoxviruses, yet its function is unknown. The unique structure of its promoter is not found for any other gene in the MVA genome and is also conserved in other orthopoxviruses. Comparison of the MVA13.5L promoter activity with synthetic poxviral promoters revealed that the MVA13.5L promoter produced higher levels of protein early during infection in HeLa cells and particularly in MDBK cells, a cell line in which MVA replication stops at an early stage before the expression of late genes. Finally, a recombinant antigen expressed under the control of this novel promoter induced high antibody titers and increased CD8 T cell responses in homologous prime-boost immunization compared to commonly used promoters. In particular, the recombinant antigen specific CD8 T cell responses dominated over the immunodominant B8R vector-specific responses after three vaccinations and even more during the memory phase. These results have identified the native MVA13.5L promoter as a new potent promoter for use in MVA vectored preventive and therapeutic vaccines. PMID:23951355

  1. Can vaccinia virus be replaced by MVA virus for testing virucidal activity of chemical disinfectants?

    Directory of Open Access Journals (Sweden)

    Rapp Ingrid

    2010-06-01

    Full Text Available Abstract Background Vaccinia virus strain Lister Elstree (VACV is a test virus in the DVV/RKI guidelines as representative of the stable enveloped viruses. Since the potential risk of laboratory-acquired infections with VACV persists and since the adverse effects of vaccination with VACV are described, the replacement of VACV by the modified vaccinia Ankara strain (MVA was studied by testing the activity of different chemical biocides in three German laboratories. Methods The inactivating properties of different chemical biocides (peracetic acid, aldehydes and alcohols were tested in a quantitative suspension test according to the DVV/RKI guideline. All tests were performed with a protein load of 10% fetal calf serum with both viruses in parallel using different concentrations and contact times. Residual virus was determined by endpoint dilution method. Results The chemical biocides exhibited similar virucidal activity against VACV and MVA. In three cases intra-laboratory differences were determined between VACV and MVA - 40% (v/v ethanol and 30% (v/v isopropanol are more active against MVA, whereas MVA seems more stable than VACV when testing with 0.05% glutardialdehyde. Test accuracy across the three participating laboratories was high. Remarkably inter-laboratory differences in the reduction factor were only observed in two cases. Conclusions Our data provide valuable information for the replacement of VACV by MVA for testing chemical biocides and disinfectants. Because MVA does not replicate in humans this would eliminate the potential risk of inadvertent inoculation with vaccinia virus and disease in non-vaccinated laboratory workers.

  2. Safety and Immunogenicity of Heterologous Prime-Boost Immunisation with Plasmodium falciparum Malaria Candidate Vaccines, ChAd63 ME-TRAP and MVA ME-TRAP, in Healthy Gambian and Kenyan Adults

    OpenAIRE

    2013-01-01

    Background Heterologous prime boost immunization with chimpanzee adenovirus 63 (ChAd63) and Modified vaccinia Virus Ankara (MVA) vectored vaccines is a strategy recently shown to be capable of inducing strong cell mediated responses against several antigens from the malaria parasite. ChAd63-MVA expressing the Plasmodium falciparum pre-erythrocytic antigen ME-TRAP (multiple epitope string with thrombospondin-related adhesion protein) is a leading malaria vaccine candidate, capable of inducing ...

  3. Safety and immunogenicity of the malaria candidate vaccines FP9 CS and MVA CS in adult Gambian men.

    Science.gov (United States)

    Imoukhuede, E B; Berthoud, T; Milligan, P; Bojang, K; Ismaili, J; Keating, S; Nwakanma, D; Keita, S; Njie, F; Sowe, M; Todryk, S; Laidlaw, S M; Skinner, M A; Lang, T; Gilbert, S; Greenwood, B M; Hill, A V S

    2006-10-30

    We assessed the safety and immunogenicity of prime-boost vectors encoding the Plasmodium falciparum circumsporozoite (CS) protein expressed either in the attenuated fowl-pox virus (FP9) or modified vaccinia virus Ankara (MVA). Thirty-two adult Gambians in groups of four to eight received one, two or three doses of FP9 CS and/or MVA CS. No serious adverse event was observed following vaccination. The most immunogenic regimen was two doses of FP9 followed by a single dose of MVA 4 weeks later (an average of 1000 IFN-gamma spot forming units/million PBMCs). This level of effector T-cell responses appears higher than that seen in previously reported studies of CS-based candidate malaria vaccines. PMID:16842888

  4. A Novel Naturally Occurring Tandem Promoter in Modified Vaccinia Virus Ankara Drives Very Early Gene Expression and Potent Immune Responses

    OpenAIRE

    Wennier, Sonia T.; Brinkmann, Kay; Steinhäußer, Charlotte; Mayländer, Nicole; Mnich, Claudia; Wielert, Ursula; Dirmeier, Ulrike; Hausmann, Jürgen; Chaplin, Paul; Steigerwald, Robin

    2013-01-01

    Modified vaccinia virus Ankara (MVA) has been shown to be suitable for the generation of experimental vaccines against cancer and infectious diseases, eliciting strong humoral and cellular immune responses. In viral vectored vaccines, strong recombinant antigen expression and timing of expression influence the quantity and quality of the immune response. Screening of synthetic and native poxvirus promoters for strong protein expression in vitro and potent immune responses in vivo led to the i...

  5. Immunogenicity and protective efficacy of recombinant Modified Vaccinia virus Ankara candidate vaccines delivering West Nile virus envelope antigens.

    Science.gov (United States)

    Volz, Asisa; Lim, Stephanie; Kaserer, Martina; Lülf, Anna; Marr, Lisa; Jany, Sylvia; Deeg, Cornelia A; Pijlman, Gorben P; Koraka, Penelope; Osterhaus, Albert D M E; Martina, Byron E; Sutter, Gerd

    2016-04-01

    West Nile virus (WNV) cycles between insects and wild birds, and is transmitted via mosquito vectors to horses and humans, potentially causing severe neuroinvasive disease. Modified Vaccinia virus Ankara (MVA) is an advanced viral vector for developing new recombinant vaccines against infectious diseases and cancer. Here, we generated and evaluated recombinant MVA candidate vaccines that deliver WNV envelope (E) antigens and fulfil all the requirements to proceed to clinical testing in humans. Infections of human and equine cell cultures with recombinant MVA demonstrated efficient synthesis and secretion of WNV envelope proteins in mammalian cells non-permissive for MVA replication. Prime-boost immunizations in BALB/c mice readily induced circulating serum antibodies binding to recombinant WNV E protein and neutralizing WNV in tissue culture infections. Vaccinations in HLA-A2.1-/HLA-DR1-transgenic H-2 class I-/class II-knockout mice elicited WNV E-specific CD8+ T cell responses. Moreover, the MVA-WNV candidate vaccines protected C57BL/6 mice against lineage 1 and lineage 2 WNV infection and induced heterologous neutralizing antibodies. Thus, further studies are warranted to evaluate these recombinant MVA-WNV vaccines in other preclinical models and use them as candidate vaccine in humans. PMID:26939903

  6. Potent Functional Antibody Responses Elicited by HIV-I DNA Priming and Boosting with Heterologous HIV-1 Recombinant MVA in Healthy Tanzanian Adults

    OpenAIRE

    Agricola Joachim; Charlotta Nilsson; Said Aboud; Muhammad Bakari; Lyamuya, Eligius F; Merlin L Robb; Marovich, Mary A.; Patricia Earl; Bernard Moss; Christina Ochsenbauer; Britta Wahren; Fred Mhalu; Eric Sandström; Gunnel Biberfeld; Guido Ferrari

    2015-01-01

    Vaccine-induced HIV antibodies were evaluated in serum samples collected from healthy Tanzanian volunteers participating in a phase I/II placebo-controlled double blind trial using multi-clade, multigene HIV-DNA priming and recombinant modified vaccinia Ankara (HIV-MVA) virus boosting (HIVIS03). The HIV-DNA vaccine contained plasmids expressing HIV-1 gp160 subtypes A, B, C, Rev B, Gag A, B and RTmut B, and the recombinant HIV-MVA boost expressed CRF01_AE HIV-1 Env subtype E and Gag-Pol subtyp...

  7. Side-by-side comparison of gene-based smallpox vaccine with MVA in nonhuman primates.

    Directory of Open Access Journals (Sweden)

    Joseph W Golden

    Full Text Available Orthopoxviruses remain a threat as biological weapons and zoonoses. The licensed live-virus vaccine is associated with serious health risks, making its general usage unacceptable. Attenuated vaccines are being developed as alternatives, the most advanced of which is modified-vaccinia virus Ankara (MVA. We previously developed a gene-based vaccine, termed 4pox, which targets four orthopoxvirus antigens, A33, B5, A27 and L1. This vaccine protects mice and non-human primates from lethal orthopoxvirus disease. Here, we investigated the capacity of the molecular adjuvants GM-CSF and Escherichia coli heat-labile enterotoxin (LT to enhance the efficacy of the 4pox gene-based vaccine. Both adjuvants significantly increased protective antibody responses in mice. We directly compared the 4pox plus LT vaccine against MVA in a monkeypox virus (MPXV nonhuman primate (NHP challenge model. NHPs were vaccinated twice with MVA by intramuscular injection or the 4pox/LT vaccine delivered using a disposable gene gun device. As a positive control, one NHP was vaccinated with ACAM2000. NHPs vaccinated with each vaccine developed anti-orthopoxvirus antibody responses, including those against the 4pox antigens. After MPXV intravenous challenge, all control NHPs developed severe disease, while the ACAM2000 vaccinated animal was well protected. All NHPs vaccinated with MVA were protected from lethality, but three of five developed severe disease and all animals shed virus. All five NHPs vaccinated with 4pox/LT survived and only one developed severe disease. None of the 4pox/LT-vaccinated animals shed virus. Our findings show, for the first time, that a subunit orthopoxvirus vaccine delivered by the same schedule can provide a degree of protection at least as high as that of MVA.

  8. A Novel Replication-Competent Vaccinia Vector MVTT Is Superior to MVA for Inducing High Levels of Neutralizing Antibody via Mucosal Vaccination

    OpenAIRE

    Huang, Xiaoxing; Lu, Bin; Yu, Wenbo; Fang, Qing; Liu, Li; Zhuang, Ke; Shen, Tingting; Wang, Haibo; Tian, Po; Zhang, Linqi; Chen, Zhiwei

    2009-01-01

    Mucosal vaccination offers great advantage for inducing protective immune response to prevent viral transmission and dissemination. Here, we report our findings of a head-to-head comparison of two viral vectors modified vaccinia Ankara (MVA) and a novel replication-competent modified vaccinia Tian Tan (MVTT) for inducing neutralizing antibodies (Nabs) via intramuscular and mucosal vaccinations in mice. MVTT is an attenuated variant of the wild-type VTT, which was historically used as a smallp...

  9. Partially randomized, non-blinded trial of DNA and MVA therapeutic vaccines based on hepatitis B virus surface protein for chronic HBV infection.

    OpenAIRE

    Cavenaugh, James S; Dorka Awi; Maimuna Mendy; Hill, Adrian V. S.; Hilton Whittle; McConkey, Samuel J.

    2011-01-01

    BACKGROUND: Chronic HBV infects 350 million people causing cancer and liver failure. We aimed to assess the safety and efficacy of plasmid DNA (pSG2.HBs) vaccine, followed by recombinant modified vaccinia virus Ankara (MVA.HBs), encoding the surface antigen of HBV as therapy for chronic HBV. A secondary goal was to characterize the immune responses. METHODS: Firstly 32 HBV e antigen negative (eAg(-)) participants were randomly assigned to one of four groups: to receive vaccines alone, lamivud...

  10. A novel replication-competent vaccinia vector MVTT is superior to MVA for inducing high levels of neutralizing antibody via mucosal vaccination

    OpenAIRE

    Xiaoxing Huang; Bin Lu; Wenbo Yu; Qing Fang; Li Liu; Ke Zhuang; Tingting Shen; Haibo Wang; Po Tian; Linqi Zhang; Zhiwei Chen

    2009-01-01

    Mucosal vaccination offers great advantage for inducing protective immune response to prevent viral transmission and dissemination. Here, we report our findings of a head-to-head comparison of two viral vectors modified vaccinia Ankara (MVA) and a novel replication-competent modified vaccinia Tian Tan (MVTT) for inducing neutralizing antibodies (Nabs) via intramuscular and mucosal vaccinations in mice. MVTT is an attenuated variant of the wild-type VTT, which was historically used as a smallp...

  11. Co-expression of Interleukin-15 Enhances the Protective Immune Responses Induced by Immunization with a Murine Malaria MVA-Based Vaccine Encoding the Circumsporozoite Protein

    OpenAIRE

    Parra, Marcela; Liu, Xia; Derrick, Steven C.; Yang, Amy; Molina-Cruz, Alvaro; Barillas-Mury, Carolina; Zheng, Hong; Thao Pham, Phuong; Sedegah, Martha; Belmonte, Arnel; Litilit, Dianne D.; Waldmann, Thomas A.; Kumar, Sanjai; Morris, Sheldon L.; Perera, Liyanage P.

    2015-01-01

    Malaria remains a major global public health problem with an estimated 200 million cases detected in 2012. Although the most advanced candidate malaria vaccine (RTS,S) has shown promise in clinical trials, its modest efficacy and durability have created uncertainty about the impact of RTS,S immunization (when used alone) on global malaria transmission. Here we describe the development and characterization of a novel modified vaccinia virus Ankara (MVA)–based malaria vaccine which co-expresses...

  12. Oral vaccination with modified vaccinia virus Ankara attached covalently to TMPEG-modified cationic liposomes overcomes pre-existing poxvirus immunity from recombinant vaccinia immunization

    OpenAIRE

    Naito, Toshio; Kaneko, Yutaro; Kozbor, Danuta

    2007-01-01

    Development of a safe and effective vaccine for induction of mucosal immunity to the human immunodeficiency virus (HIV) envelope glycoprotein (Env, gp160) represents the best hope for containing the spread of an HIV epidemic worldwide. The highly attenuated modified vaccinia virus Ankara (MVA) is a laboratory virus well suited as a safe vaccine vector. However, the presence of pre-existing immunity to Vaccinia virus in the adult population represents a hindrance that limits the application of...

  13. DNA/MVA Vaccines for HIV/AIDS

    Directory of Open Access Journals (Sweden)

    Smita S. Iyer

    2014-02-01

    Full Text Available Since the initial proof-of-concept studies examining the ability of antigen-encoded plasmid DNA to serve as an immunogen, DNA vaccines have evolved as a clinically safe and effective platform for priming HIV-specific cellular and humoral responses in heterologous “prime-boost” vaccination regimens. Direct injection of plasmid DNA into the muscle induces T- and B-cell responses against foreign antigens. However, the insufficient magnitude of this response has led to the development of approaches for enhancing the immunogenicity of DNA vaccines. The last two decades have seen significant progress in the DNA-based vaccine platform with optimized plasmid constructs, improved delivery methods, such as electroporation, the use of molecular adjuvants and novel strategies combining DNA with viral vectors and subunit proteins. These innovations are paving the way for the clinical application of DNA-based HIV vaccines. Here, we review preclinical studies on the DNA-prime/modified vaccinia Ankara (MVA-boost vaccine modality for HIV. There is a great deal of interest in enhancing the immunogenicity of DNA by engineering DNA vaccines to co-express immune modulatory adjuvants. Some of these adjuvants have demonstrated encouraging results in preclinical and clinical studies, and these data will be examined, as well.

  14. Market implementation of the MVA platform for pre-pandemic and pandemic influenza vaccines: A quantitative key opinion leader analysis

    Science.gov (United States)

    Ramezanpour, Bahar; Pronker, Esther S.; Kreijtz, Joost H.C.M.; Osterhaus, Albert D.M.E.; Claassen, E.

    2015-01-01

    A quantitative method is presented to rank strengths, weaknesses, opportunities, and threats (SWOT) of modified vaccinia virus Ankara (MVA) as a platform for pre-pandemic and pandemic influenza vaccines. Analytic hierarchy process (AHP) was applied to achieve pairwise comparisons among SWOT factors in order to prioritize them. Key opinion leaders (KOLs) in the influenza vaccine field were interviewed to collect a unique dataset to evaluate the market potential of this platform. The purpose of this study, to evaluate commercial potential of the MVA platform for the development of novel generation pandemic influenza vaccines, is accomplished by using a SWOT and AHP combined analytic method. Application of the SWOT–AHP model indicates that its strengths are considered more important by KOLs than its weaknesses, opportunities, and threats. Particularly, the inherent immunogenicity capability of MVA without the requirement of an adjuvant is the most important factor to increase commercial attractiveness of this platform. Concerns regarding vector vaccines and anti-vector immunity are considered its most important weakness, which might lower public health value of this platform. Furthermore, evaluation of the results of this study emphasizes equally important role that threats and opportunities of this platform play. This study further highlights unmet needs in the influenza vaccine market, which could be addressed by the implementation of the MVA platform. Broad use of MVA in clinical trials shows great promise for this vector as vaccine platform for pre-pandemic and pandemic influenza and threats by other respiratory viruses. Moreover, from the results of the clinical trials seem that MVA is particularly attractive for development of vaccines against pathogens for which no, or only insufficiently effective vaccines, are available. PMID:26048779

  15. Expression and Cellular Immunogenicity of a Transgenic Antigen Driven by Endogenous Poxviral Early Promoters at Their Authentic Loci in MVA

    Science.gov (United States)

    Orubu, Toritse; Alharbi, Naif Khalaf; Lambe, Teresa; Gilbert, Sarah C.; Cottingham, Matthew G.

    2012-01-01

    CD8+ T cell responses to vaccinia virus are directed almost exclusively against early gene products. The attenuated strain modified vaccinia virus Ankara (MVA) is under evaluation in clinical trials of new vaccines designed to elicit cellular immune responses against pathogens including Plasmodium spp., M. tuberculosis and HIV-1. All of these recombinant MVAs (rMVA) utilize the well-established method of linking the gene of interest to a cloned poxviral promoter prior to insertion into the viral genome at a suitable locus by homologous recombination in infected cells. Using BAC recombineering, we show that potent early promoters that drive expression of non-functional or non-essential MVA open reading frames (ORFs) can be harnessed for immunogenic expression of recombinant antigen. Precise replacement of the MVA orthologs of C11R, F11L, A44L and B8R with a model antigen positioned to use the same translation initiation codon allowed early transgene expression similar to or slightly greater than that achieved by the commonly-used p7.5 or short synthetic promoters. The frequency of antigen-specific CD8+ T cells induced in mice by single shot or adenovirus-prime, rMVA-boost vaccination were similarly equal or marginally enhanced using endogenous promoters at their authentic genomic loci compared to the traditional constructs. The enhancement in immunogenicity observed using the C11R or F11L promoters compared with p7.5 was similar to that obtained with the mH5 promoter compared with p7.5. Furthermore, the growth rates of the viruses were unimpaired and the insertions were genetically stable. Insertion of a transgenic ORF in place of a viral ORF by BAC recombineering can thus provide not only a potent promoter, but also, concomitantly, a suitable insertion site, potentially facilitating development of MVA vaccines expressing multiple recombinant antigens. PMID:22761956

  16. Expression and cellular immunogenicity of a transgenic antigen driven by endogenous poxviral early promoters at their authentic loci in MVA.

    Directory of Open Access Journals (Sweden)

    Toritse Orubu

    Full Text Available CD8(+ T cell responses to vaccinia virus are directed almost exclusively against early gene products. The attenuated strain modified vaccinia virus Ankara (MVA is under evaluation in clinical trials of new vaccines designed to elicit cellular immune responses against pathogens including Plasmodium spp., M. tuberculosis and HIV-1. All of these recombinant MVAs (rMVA utilize the well-established method of linking the gene of interest to a cloned poxviral promoter prior to insertion into the viral genome at a suitable locus by homologous recombination in infected cells. Using BAC recombineering, we show that potent early promoters that drive expression of non-functional or non-essential MVA open reading frames (ORFs can be harnessed for immunogenic expression of recombinant antigen. Precise replacement of the MVA orthologs of C11R, F11L, A44L and B8R with a model antigen positioned to use the same translation initiation codon allowed early transgene expression similar to or slightly greater than that achieved by the commonly-used p7.5 or short synthetic promoters. The frequency of antigen-specific CD8(+ T cells induced in mice by single shot or adenovirus-prime, rMVA-boost vaccination were similarly equal or marginally enhanced using endogenous promoters at their authentic genomic loci compared to the traditional constructs. The enhancement in immunogenicity observed using the C11R or F11L promoters compared with p7.5 was similar to that obtained with the mH5 promoter compared with p7.5. Furthermore, the growth rates of the viruses were unimpaired and the insertions were genetically stable. Insertion of a transgenic ORF in place of a viral ORF by BAC recombineering can thus provide not only a potent promoter, but also, concomitantly, a suitable insertion site, potentially facilitating development of MVA vaccines expressing multiple recombinant antigens.

  17. Market implementation of the MVA platform for pre-pandemic and pandemic influenza vaccines: A quantitative key opinion leader analysis.

    Science.gov (United States)

    Ramezanpour, Bahar; Pronker, Esther S; Kreijtz, Joost H C M; Osterhaus, Albert D M E; Claassen, E

    2015-08-20

    A quantitative method is presented to rank strengths, weaknesses, opportunities, and threats (SWOT) of modified vaccinia virus Ankara (MVA) as a platform for pre-pandemic and pandemic influenza vaccines. Analytic hierarchy process (AHP) was applied to achieve pairwise comparisons among SWOT factors in order to prioritize them. Key opinion leaders (KOLs) in the influenza vaccine field were interviewed to collect a unique dataset to evaluate the market potential of this platform. The purpose of this study, to evaluate commercial potential of the MVA platform for the development of novel generation pandemic influenza vaccines, is accomplished by using a SWOT and AHP combined analytic method. Application of the SWOT-AHP model indicates that its strengths are considered more important by KOLs than its weaknesses, opportunities, and threats. Particularly, the inherent immunogenicity capability of MVA without the requirement of an adjuvant is the most important factor to increase commercial attractiveness of this platform. Concerns regarding vector vaccines and anti-vector immunity are considered its most important weakness, which might lower public health value of this platform. Furthermore, evaluation of the results of this study emphasizes equally important role that threats and opportunities of this platform play. This study further highlights unmet needs in the influenza vaccine market, which could be addressed by the implementation of the MVA platform. Broad use of MVA in clinical trials shows great promise for this vector as vaccine platform for pre-pandemic and pandemic influenza and threats by other respiratory viruses. Moreover, from the results of the clinical trials seem that MVA is particularly attractive for development of vaccines against pathogens for which no, or only insufficiently effective vaccines, are available. PMID:26048779

  18. Identification of pre- and post-treatment markers, clinical, and laboratory parameters associated with outcome in renal cancer patients treated with MVA-5T4

    Directory of Open Access Journals (Sweden)

    RobertAmato

    2013-07-01

    Full Text Available The recent approvals of immunotherapeutic agents (Sipuleucel-T and Ipilimumab for the treatment of different solid tumors gave a boost to the growing cancer immunotherapy field, even though few immunotherapy studies have demonstrated convincingly that there is a direct link between the predicted mode of action of an immunological compound and therapeutic benefit. MVA-5T4 (Trovax® is a novel vaccine combining the tumor-associated antigen 5T4 to an engineered vector-modified vaccinia Ankara (MVA. MVA helps to express the oncofetal 5T4 antigen and subsequently trigger a tumor-directed immune reaction. The safety and clinical benefit reported in multiple phase I and II clinical trials using MVA-5T4 were encouraging; immune responses were induced in almost all treated patients, and associations between 5T4-specific cellular or humoral responses and clinical benefit were reported in most of the nine phase II trials. In particular, clinical studies conducted in renal cell carcinoma (RCC patients have demonstrated an association between 5T4-specific (but not MVA antibody responses and enhanced survival. This review describes the clinical studies using MVA-5T4 conducted in RCC that convincingly demonstrated that an antigen-specific immune response induced by vaccination is associated with enhanced patient survival and is not simply a function of the general “health” of patients. We will also provide our expert opinions on possible future better-designed clinical trials based on relevant biomarkers. In addition, various combinations of MVA-5T4 and different and newer immunomodulator agents with promising clinical benefit will be discussed.

  19. Low pathogenic avian influenza (H9N2) in chicken: Evaluation of an ancestral H9-MVA vaccine.

    Science.gov (United States)

    Ducatez, Mariette F; Becker, Jens; Freudenstein, Astrid; Delverdier, Maxence; Delpont, Mattias; Sutter, Gerd; Guérin, Jean-Luc; Volz, Asisa

    2016-06-30

    Modified Vaccinia Ankara (MVA) has proven its efficacy as a recombinant vector vaccine for numerous pathogens including influenza virus. The present study aimed at evaluating a recombinant MVA candidate vaccine against low pathogenic avian influenza virus subtype H9N2 in the chicken model. As the high genetic and antigenic diversity of H9N2 viruses increases vaccine design complexity, one strategy to widen the range of vaccine coverage is to use an ancestor sequence. We therefore generated a recombinant MVA encoding for the gene sequence of an ancestral hemagglutinin H9 protein (a computationally derived amino acid sequence of the node of the H9N2 G1 lineage strains was obtained using the ANCESCON program). We analyzed the genetics and the growth properties of the MVA vector virus confirming suitability for use under biosafety level 1 and tested its efficacy when applied either as an intra-muscular (IM) or an oral vaccine in specific pathogen free chickens challenged with A/chicken/Tunisia/12/2010(H9N2). Two control groups were studied in parallel (unvaccinated and inoculated birds; unvaccinated and non-inoculated birds). IM vaccinated birds seroconverted as early as four days post vaccination and neutralizing antibodies were detected against A/chicken/Tunisia/12/2010(H9N2) in all the birds before challenge. The role of local mucosal immunity is unclear here as no antibodies were detected in eye drop or aerosol vaccinated birds. Clinical signs were not detected in any of the infected birds even in absence of vaccination. Virus replication was observed in both vaccinated and unvaccinated chickens, suggesting the MVA-ancestral H9 vaccine may not stop virus spread in the field. However vaccinated birds showed less histological damage, fewer influenza-positive cells and shorter virus shedding than their unvaccinated counterparts. PMID:27259828

  20. Improving the MVA vaccine potential by deleting the viral gene coding for the IL-18 binding protein.

    Directory of Open Access Journals (Sweden)

    Juliana Falivene

    Full Text Available BACKGROUND: Modified Vaccinia Ankara (MVA is an attenuated strain of Vaccinia virus (VACV currently employed in many clinical trials against HIV/AIDS and other diseases. MVA still retains genes involved in host immune response evasion, enabling its optimization by removing some of them. The aim of this study was to evaluate cellular immune responses (CIR induced by an IL-18 binding protein gene (C12L deleted vector (MVAΔC12L. METHODOLOGY/PRINCIPAL FINDINGS: BALB/c and C57BL/6 mice were immunized with different doses of MVAΔC12L or MVA wild type (MVAwt, then CIR to VACV epitopes in immunogenic proteins were evaluated in spleen and draining lymph nodes at acute and memory phases (7 and 40 days post-immunization respectively. Compared with parental MVAwt, MVAΔC12L immunization induced a significant increase of two to three-fold in CD8(+ and CD4(+ T-cell responses to different VACV epitopes, with increased percentage of anti-VACV cytotoxic CD8(+ T-cells (CD107a/b(+ during the acute phase of the response. Importantly, the immunogenicity enhancement was also observed after MVAΔC12L inoculation with different viral doses and by distinct routes (systemic and mucosal. Potentiation of MVA's CIR was also observed during the memory phase, in correlation with a higher protection against an intranasal challenge with VACV WR. Of note, we could also show a significant increase in the CIR against HIV antigens such as Env, Gag, Pol and Nef from different subtypes expressed from two recombinants of MVAΔC12L during heterologous DNA prime/MVA boost vaccination regimens. CONCLUSIONS/SIGNIFICANCE: This study demonstrates the relevance of IL-18 bp contribution in the immune response evasion during MVA infection. Our findings clearly show that the deletion of the viral IL-18 bp gene is an effective approach to increase MVA vaccine efficacy, as immunogenicity improvements were observed against vector antigens and more importantly to HIV antigens.

  1. Chimpanzee adenovirus- and MVA-vectored respiratory syncytial virus vaccine is safe and immunogenic in adults.

    Science.gov (United States)

    Green, Christopher A; Scarselli, Elisa; Sande, Charles J; Thompson, Amber J; de Lara, Catherine M; Taylor, Kathryn S; Haworth, Kathryn; Del Sorbo, Mariarosaria; Angus, Brian; Siani, Loredana; Di Marco, Stefania; Traboni, Cinzia; Folgori, Antonella; Colloca, Stefano; Capone, Stefania; Vitelli, Alessandra; Cortese, Riccardo; Klenerman, Paul; Nicosia, Alfredo; Pollard, Andrew J

    2015-08-12

    Respiratory syncytial virus (RSV) causes respiratory infection in annual epidemics, with infants and the elderly at particular risk of developing severe disease and death. However, despite its importance, no vaccine exists. The chimpanzee adenovirus, PanAd3-RSV, and modified vaccinia virus Ankara, MVA-RSV, are replication-defective viral vectors encoding the RSV fusion (F), nucleocapsid (N), and matrix (M2-1) proteins for the induction of humoral and cellular responses. We performed an open-label, dose escalation, phase 1 clinical trial in 42 healthy adults in which four different combinations of prime/boost vaccinations were investigated for safety and immunogenicity, including both intramuscular (IM) and intranasal (IN) administration of the adenovirus-vectored vaccine. The vaccines were safe and well tolerated, with the most common reported adverse events being mild injection site reactions. No vaccine-related serious adverse events occurred. RSV neutralizing antibody titers rose in response to IM prime with PanAd3-RSV and after IM boost for individuals primed by the IN route. Circulating anti-F immunoglobulin G (IgG) and IgA antibody-secreting cells (ASCs) were observed after the IM prime and IM boost. RSV-specific T cell responses were increased after the IM PanAd3-RSV prime and were most efficiently boosted by IM MVA-RSV. Interferon-γ (IFN-γ) secretion after boost was from both CD4(+) and CD8(+) T cells, without detectable T helper cell 2 (TH2) cytokines that have been previously associated with immune pathogenesis following exposure to RSV after the formalin-inactivated RSV vaccine. In conclusion, PanAd3-RSV and MVA-RSV are safe and immunogenic in healthy adults. These vaccine candidates warrant further clinical evaluation of efficacy to assess their potential to reduce the burden of RSV disease. PMID:26268313

  2. A fusion protein of HCMV IE1 exon4 and IE2 exon5 stimulates potent cellular immunity in an MVA vaccine vector

    International Nuclear Information System (INIS)

    A therapeutic CMV vaccine incorporating an antigenic repertoire capable of eliciting a cellular immune response has yet to be successfully implemented for patients who already have acquired an infection. To address this problem, we have developed a vaccine candidate derived from modified vaccinia Ankara (MVA) that expresses three immunodominant antigens (pp65, IE1, IE2) from CMV. The novelty of this vaccine is the fusion of two adjacent exons from the immediate-early region of CMV, their successful expression in MVA, and robust immunogenicity in both primary and memory response models. Evaluation of the immunogenicity of the viral vaccine in mouse models shows that it can stimulate primary immunity against all three antigens in both the CD4+ and CD8+ T cell subsets. Evaluation of human PBMC from healthy CMV-positive donors or patients within 6 months of receiving hematopoietic cell transplant shows robust stimulation of existing CMV-specific CD4+ and CD8+ T cell subsets

  3. FWI and MVA the natural way

    KAUST Repository

    Alkhalifah, Tariq Ali

    2014-01-01

    Integrating migration velocity analysis (MVA) and full waveform inversion (FWI) can help reduce the high nonlinearity of the classic FWI objective function. The combination of inverting for the long and short wavelength components of the velocity model using a dual objective function that is sensitive to both components is still very expensive and have produced mixed results. We develop an approach that includes both components integrated to complement each other. We specifically utilize the image to generate reflections in our synthetic data only when the velocity model is not capable of producing such reflections. As a result, we get the MVA working when we need it, and mitigate it\\'s influence when the velocity model produces accurate reflections (possible first for the low frequencies). Applications to a layered model, as well as, the Marmousi model demonstrate some of the approach features.

  4. Recombinant modified vaccinia virus Ankara expressing glycoprotein E2 of Chikungunya virus protects AG129 mice against lethal challenge.

    Directory of Open Access Journals (Sweden)

    Petra van den Doel

    2014-09-01

    Full Text Available Chikungunya virus (CHIKV infection is characterized by rash, acute high fever, chills, headache, nausea, photophobia, vomiting, and severe polyarthralgia. There is evidence that arthralgia can persist for years and result in long-term discomfort. Neurologic disease with fatal outcome has been documented, although at low incidences. The CHIKV RNA genome encodes five structural proteins (C, E1, E2, E3 and 6K. The E1 spike protein drives the fusion process within the cytoplasm, while the E2 protein is believed to interact with cellular receptors and therefore most probably constitutes the target of neutralizing antibodies. We have constructed recombinant Modified Vaccinia Ankara (MVA expressing E3E2, 6KE1, or the entire CHIKV envelope polyprotein cassette E3E26KE1. MVA is an appropriate platform because of its demonstrated clinical safety and its suitability for expression of various heterologous proteins. After completing the immunization scheme, animals were challenged with CHIV-S27. Immunization of AG129 mice with MVAs expressing E2 or E3E26KE1 elicited neutralizing antibodies in all animals and provided 100% protection against lethal disease. In contrast, 75% of the animals immunized with 6KE1 were protected against lethal infection. In conclusion, MVA expressing the glycoprotein E2 of CHIKV represents as an immunogenic and effective candidate vaccine against CHIKV infections.

  5. Protective effects of a Modified Vaccinia Ankara-based vaccine candidate against Crimean-Congo Haemorrhagic Fever virus require both cellular and humoral responses

    Science.gov (United States)

    Dowall, Stuart D.; Graham, Victoria A.; Rayner, Emma; Hunter, Laura; Watson, Robert; Taylor, Irene; Rule, Antony; Carroll, Miles W.; Hewson, Roger

    2016-01-01

    Crimean-Congo Haemorrhagic Fever (CCHF) is a severe tick-borne disease, endemic in many countries in Africa, the Middle East, Eastern Europe and Asia. There is no approved vaccine currently available against CCHF. The most promising candidate, which has previously been shown to confer protection in the small animal model, is a modified Vaccinia Ankara virus vector expressing the CCHF viral glycoprotein (MVA-GP). It has been shown that MVA-GP induces both humoral and cellular immunogenicity. In the present study, sera and T-lymphocytes were passively and adoptively transferred into recipient mice prior to challenge with CCHF virus. Results demonstrated that mediators from both arms of the immune system were required to demonstrate protective effects against lethal challenge. PMID:27272940

  6. Comparing adjuvanted H28 and modified vaccinia virus ankara expressingH28 in a mouse and a non-human primate tuberculosis model

    DEFF Research Database (Denmark)

    Billeskov, Rolf; Christensen, Jan Pravsgaard; Aagaard, Claus;

    2013-01-01

    Here we report for the first time on the immunogenicity and protective efficacy of a vaccine strategy involving the adjuvanted fusion protein "H28" (consisting of Ag85B-TB10.4-Rv2660c) and Modified Vaccinia Virus Ankara expressing H28. We show that a heterologous prime-boost regimen involving...... a significant positive correlation with protection at week 6 post infection, whereas the opposite was observed for post infection CD4 T cells producing only IFN-γ. Moreover, as a BCG booster vaccine in a clinically relevant non-human primate TB model, the H28/H28 vaccine strategy induced a slightly...... more prominent reduction of clinical disease and pathology for up to one year post infection compared to H28/MVA28. Taken together, our data showed that the adjuvanted subunit and MVA strategies led to different T cell subset combinations pre and post infection and that TNF-α/IL-2 double producing but...

  7. Protective effects of a Modified Vaccinia Ankara-based vaccine candidate against Crimean-Congo Haemorrhagic Fever virus require both cellular and humoral responses.

    Directory of Open Access Journals (Sweden)

    Stuart D Dowall

    Full Text Available Crimean-Congo Haemorrhagic Fever (CCHF is a severe tick-borne disease, endemic in many countries in Africa, the Middle East, Eastern Europe and Asia. There is no approved vaccine currently available against CCHF. The most promising candidate, which has previously been shown to confer protection in the small animal model, is a modified Vaccinia Ankara virus vector expressing the CCHF viral glycoprotein (MVA-GP. It has been shown that MVA-GP induces both humoral and cellular immunogenicity. In the present study, sera and T-lymphocytes were passively and adoptively transferred into recipient mice prior to challenge with CCHF virus. Results demonstrated that mediators from both arms of the immune system were required to demonstrate protective effects against lethal challenge.

  8. 19th Century Ankara Through Historical Poems

    Directory of Open Access Journals (Sweden)

    Özge Öztekin

    2015-12-01

    Full Text Available A city is a place whose meaning is found in the poetry created there. In Kevin Lynch’s words, a city presents the imagination with an unlimited potential for “readability”. If we consider this unlimited readability through poetry, it can be said that attempts to find the zeitgeist of a city at a certain time through literary texts must evaluate the poetry, the city and the time. This is because poetry (or literature in general, just like a city, has an important memory which oscillates through ideas of its past and future. In this sense, divan poetry and one particular example of it—historical “manzume” poems—are memories which richly illustrate the ‘continuity’ and ‘change’ within a period. This work, on 19th century Ankara, aims to evaluate the traces reflected in historical manzume poems of the time they were written. Five historical manzume poems in three texts out of seventy 19th century divan collections scanned for this work were found to be about Ankara. Two of these manzumes are by Cazib, one by Ziver Pasha, and one by Mahmud Celaleddin Pasha. The first of these is on Ankara’s dervish lodge; the second on a barracks being built in Ankara; the third on Vecihi Pasha’s governorship of Ankara; the fourth on the the Mayoral Residence. In addition to these, a manzume on the construction of Hamidiye Caddesi by Mahmud Celaleddin Pasha is discovered with in scope of the work. The aim of this work is to provide a contribution to city history through a commentary on elements of 19th century poetry concerning Ankara.

  9. Double-Difference Tomography for Sequestration MVA

    Energy Technology Data Exchange (ETDEWEB)

    Westman, Erik

    2008-12-31

    Analysis of synthetic data was performed to determine the most cost-effective tomographic monitoring system for a geologic carbon sequestration injection site. Double-difference tomographic inversion was performed on 125 synthetic data sets: five stages of CO2 plume growth, five seismic event regions, and five geophone arrays. Each resulting velocity model was compared quantitatively to its respective synthetic velocity model to determine an accuracy value. The results were examined to determine a relationship between cost and accuracy in monitoring, verification, and accounting applications using double-difference tomography. The geophone arrays with widely-varying geophone locations, both laterally and vertically, performed best. Additionally, double difference seismic tomography was performed using travel time data from a carbon sequestration site at the Aneth oil field in southeast Utah as part of a Department of Energy initiative on monitoring, verification, and accounting (MVA) of sequestered CO2. A total of 1,211 seismic events were recorded from a borehole array consisting of 22 geophones. Artificial velocity models were created to determine the ease with which different CO2 plume locations and sizes can be detected. Most likely because of the poor geophone arrangement, a low velocity zone in the Desert Creek reservoir can only be detected when regions of test site containing the highest ray path coverage are considered. MVA accuracy and precision may be improved through the use of a receiver array that provides more comprehensive ray path coverage.

  10. A Photographer From Ankara: Osman Darcan

    Directory of Open Access Journals (Sweden)

    Gülseren Mungan Yavuztürk

    2015-12-01

    Full Text Available This work introduces Osman Darcan, an important name in the history of Ankara photography studios. Darcan followed in the footsteps of famous Austrian photographer Othmar Pferschy, whom he met in Istanbul, to go on to create his own valuable work. On leaving the Public Press Authority Photo Film Center, where he worked as a newsreel photographer and film operator, in 1943 he began taking photographs at the Tatbikat Theater at the Ankara State Conservatoire, where he continued as the photographer for the State Theater until the end of his life. At the same time, this master photographer took the pictures of a select coterie of Ankara’s leading individuals and well-known performers at a studio he opened on Anafartalar Caddesi. In both these roles, his photographs evoke admiration thanks to Darcan’s professional abilities and level of artistry.

  11. Induction of Antibody Responses to African Horse Sickness Virus (AHSV) in Ponies after Vaccination with Recombinant Modified Vaccinia Ankara (MVA)

    OpenAIRE

    Chiam, Rachael; Sharp, Emma; Maan, Sushila; Rao, Shujing; Mertens, Peter; Blacklaws, Barbara; Davis-Poynter, Nick; Wood, James; Castillo-Olivares, Javier

    2009-01-01

    Background African horse sickness virus (AHSV) causes a non-contagious, infectious disease in equids, with mortality rates that can exceed 90% in susceptible horse populations. AHSV vaccines play a crucial role in the control of the disease; however, there are concerns over the use of polyvalent live attenuated vaccines particularly in areas where AHSV is not endemic. Therefore, it is important to consider alternative approaches for AHSV vaccine development. We have carried out a pilot study ...

  12. Strong, but Age-Dependent, Protection Elicited by a Deoxyribonucleic Acid/Modified Vaccinia Ankara Simian Immunodeficiency Virus Vaccine.

    Science.gov (United States)

    Chamcha, Venkateswarlu; Kannanganat, Sunil; Gangadhara, Sailaja; Nabi, Rafiq; Kozlowski, Pamela A; Montefiori, David C; LaBranche, Celia C; Wrammert, Jens; Keele, Brandon F; Balachandran, Harikrishnan; Sahu, Sujata; Lifton, Michelle; Santra, Sampa; Basu, Rahul; Moss, Bernard; Robinson, Harriet L; Amara, Rama Rao

    2016-01-01

    Background.  In this study, we analyzed the protective efficacy of a simian immunodeficiency virus (SIV) macaque 239 (SIVmac239) analogue of the clinically tested GOVX-B11 deoxyribonucleic acid (DNA)/modified vaccinia Ankara (MVA) human immunodeficiency virus vaccine. Methods.  The tested vaccine used a DNA immunogen mutated to mimic the human vaccine and a regimen with DNA deliveries at weeks 0 and 8 and MVA deliveries at weeks 16 and 32. Twelve weekly rectal challenges with 0.3 animal infectious doses of SIV sootey mangabey E660 (SIVsmE660) were administered starting at 6 months after the last immunization. Results.  Over the first 6 rectal exposures to SIVsmE660, <10-year-old tripartite motif-containing protein 5 (TRIM5)α-permissive rhesus macaques showed an 80% reduction in per-exposure risk of infection as opposed to a 46% reduction in animals over 10 years old; and, over the 12 challenges, they showed a 72% as opposed to a 10% reduction. Analyses of elicited immune responses suggested that higher antibody responses in the younger animals had played a role in protection. Conclusions.  The simian analogue of the GOVX-B11 HIV provided strong protection against repeated rectal challenges in young adult macaques. PMID:27006959

  13. Immunological characterization of a modified vaccinia virus Ankara vector expressing the human papillomavirus 16 E1 protein.

    Science.gov (United States)

    Remy-Ziller, Christelle; Germain, Claire; Spindler, Anita; Hoffmann, Chantal; Silvestre, Nathalie; Rooke, Ronald; Bonnefoy, Jean-Yves; Préville, Xavier

    2014-02-01

    Women showing normal cytology but diagnosed with a persistent high-risk human papillomavirus (HR-HPV) infection have a higher risk of developing high-grade cervical intraepithelial neoplasia and cervical cancer than noninfected women. As no therapeutic management other than surveillance is offered to these women, there is a major challenge to develop novel targeted therapies dedicated to the treatment of these patients. As such, E1 and E2 antigens, expressed early in the HPV life cycle, represent very interesting candidates. Both proteins are necessary for maintaining coordinated viral replication and gene synthesis during the differentiation process of the epithelium and are essential for the virus to complete its normal and propagative replication cycle. In the present study, we evaluated a new active targeted immunotherapeutic, a modified vaccinia virus Ankara (MVA) vector containing the E1 sequence of HPV16, aimed at inducing cellular immune responses with the potential to help and clear persistent HPV16-related infection. We carried out an extensive comparative time course analysis of the cellular immune responses induced by different schedules of immunization in C57BL/6 mice. We showed that multiple injections of MVA-E1 allowed sustained HPV16 E1-specific cellular immune responses in vaccinated mice and had no impact on the exhaustion phenotype of the generated HPV16 E1-specific CD8⁺ T cells, but they led to the differentiation of multifunctional effector T cells with high cytotoxic capacity. This study provides proof of concept that an MVA expressing HPV16 E1 can induce robust and long-lasting E1-specific responses and warrants further development of this candidate. PMID:24307238

  14. A novel replication-competent vaccinia vector MVTT is superior to MVA for inducing high levels of neutralizing antibody via mucosal vaccination.

    Directory of Open Access Journals (Sweden)

    Xiaoxing Huang

    Full Text Available Mucosal vaccination offers great advantage for inducing protective immune response to prevent viral transmission and dissemination. Here, we report our findings of a head-to-head comparison of two viral vectors modified vaccinia Ankara (MVA and a novel replication-competent modified vaccinia Tian Tan (MVTT for inducing neutralizing antibodies (Nabs via intramuscular and mucosal vaccinations in mice. MVTT is an attenuated variant of the wild-type VTT, which was historically used as a smallpox vaccine for millions of Chinese people. The spike glycoprotein (S of SARS-CoV was used as the test antigen after the S gene was constructed in the identical genomic location of two vectors to generate vaccine candidates MVTT-S and MVA-S. Using identical doses, MVTT-S induced lower levels ( approximately 2-3-fold of anti- SARS-CoV neutralizing antibodies (Nabs than MVA-S through intramuscular inoculation. MVTT-S, however, was capable of inducing consistently 20-to-100-fold higher levels of Nabs than MVA-S when inoculated via either intranasal or intraoral routes. These levels of MVTT-S-induced Nab responses were substantially (approximately 10-fold higher than that induced via the intramuscular route in the same experiments. Moreover, pre-exposure to the wild-type VTT via intranasal or intraoral route impaired the Nab response via the same routes of MVTT-S vaccination probably due to the pre-existing anti-VTT Nab response. The efficacy of intranasal or intraoral vaccination, however, was still 20-to-50-fold better than intramuscular inoculation despite the subcutaneous pre-exposure to wild-type VTT. Our data have implications for people who maintain low levels of anti-VTT Nabs after historical smallpox vaccination. MVTT is therefore an attractive live viral vector for mucosal vaccination.

  15. Development and Test of a Prototype 100MVA Superconducting Generator

    Energy Technology Data Exchange (ETDEWEB)

    Fogarty, James M.; Bray, James W.

    2007-05-25

    In 2002, General Electric and the US Department of Energy (DOE) entered into a cooperative agreement for the development of a commercialized 100 MVA generator using high temperature superconductors (HTS) in the field winding. The intent of the program was to: • Identify and develop technologies that would be needed for such a generator. • Develop conceptual designs for generators with ratings of 100 MVA and higher using HTS technology. • Perform proof of concept tests at the 1.5 MW level for GE’s proprietary warm iron rotor HTS generator concept. • Design, build, and test a prototype of a commercially viable 100 MVA generator that could be placed on the power grid. This report summarizes work performed during the program and is provided as one of the final program deliverables.

  16. Human bioclimatology analysis of Ankara urban area

    Directory of Open Access Journals (Sweden)

    Onur Çalışkan

    2011-10-01

    Full Text Available In this study, the Physiological Equivalent Temperatures (PET of different land patterns in the Ankara urban area has been analyzed. The spatial distribution and temporal variation of the thermal perceptions and the grades of thermal stress caused by the thermal conditions have been determined for 00:00, 03:00, 06:00, 09:00, 12:00, 15:00, 18:00 and 21:00 hours during the December and July of 2010. The effects of physiographic features such as elevation, aspect, slope, and especially land use, on the PETs have also been examined. According to the analysis which is carried out by using PET thermal perception index, in the research area the thermal comfort values varies around from -6 to -11°C in winter and from 13°C to 50°C in summer. In both periods the open spaces (grasslands of the city forests have the maximum PET values during the day. Either in December and in July the urban area (city center has and cool island during the day and has a heat island during the night.

  17. A Human Vaccine Strategy Based On Chimpanzee Adenoviral and MVA Vectors That Primes, Boosts and Sustains Functional HCV Specific T-Cell Memory*

    Science.gov (United States)

    Swadling, Leo; Capone, Stefania; Antrobus, Richard D.; Brown, Anthony; Richardson, Rachel; Newell, Evan W.; Halliday, John; Kelly, Christabel; Bowen, Dan; Fergusson, Joannah; Kurioka, Ayako; Ammendola, Virginia; Sorbo, Mariarosaria Del; Grazioli, Fabiana; Esposito, Maria Luisa; Siani, Loredana; Traboni, Cinzia; Hill, Adrian; Colloca, Stefano; Davis, Mark; Nicosia, Alfredo; Cortese, Riccardo; Folgori, Antonella; Klenerman, Paul; Barnes, Eleanor

    2015-01-01

    A protective vaccine against hepatitis C virus (HCV) remains an unmet clinical need. HCV infects millions of people worldwide and is a leading cause of liver cirrhosis and hepatocellular cancer. Animal challenge experiments, immunogenetics studies and assessment of host immunity during acute infection highlight the critical role that effective T-cell immunity plays in viral control. In this first-in-man study we have induced antiviral immunity with functional characteristics analogous to those associated with viral control in natural infection, and improved upon a vaccine based on adenoviral vectors alone. We assessed a heterologous prime-boost vaccination strategy based on a replicative defective simian adenoviral vector (ChAd3) and modified vaccinia Ankara (MVA) vector encoding the NS3, NS4, NS5A and NS5B proteins of HCV genotype-1b. Analysis employed single cell mass cytometry (CyTOF), and HLA class-I peptide tetramer technology in healthy human volunteers. We show that HCV specific T-cells induced by ChAd3 are optimally boosted with MVA, and generate very high levels of both CD8+ and CD4+ HCV specific T-cells targeting multiple HCV antigens. Sustained memory and effector T-cell populations are generated and T-cell memory evolved over time with improvement of quality (proliferation and polyfunctionality) following heterologous MVA boost. We have developed a HCV vaccine strategy, with durable, broad, sustained and balanced T-cell responses, characteristic of those associated with viral control, paving the way for the first efficacy studies of a prophylactic HCV vaccine. PMID:25378645

  18. Mucosal immunization with PLGA-microencapsulated DNA primes a SIV-specific CTL response revealed by boosting with cognate recombinant modified vaccinia virus Ankara

    International Nuclear Information System (INIS)

    Systemically administered DNA encoding a recombinant human immunodeficiency virus (HIV) derived immunogen effectively primes a cytotoxic T lymphocyte (CTL) response in macaques. In this further pilot study we have evaluated mucosal delivery of DNA as an alternative priming strategy. Plasmid DNA, pTH.HW, encoding a multi-CTL epitope gene, was incorporated into poly(D,L-lactic-co-glycolic acid) microparticles of less than 10 μm in diameter. Five intrarectal immunizations failed to stimulate a circulating vaccine-specific CTL response in 2 Mamu-A*01+ rhesus macaques. However, 1 week after intradermal immunization with a cognate modified vaccinia virus Ankara vaccine MVA.HW, CTL responses were detected in both animals that persisted until analysis postmortem, 12 weeks after the final boost. In contrast, a weaker and less durable response was seen in an animal vaccinated with the MVA construct alone. Analysis of lymphoid tissues revealed a disseminated CTL response in peripheral and regional lymph nodes but not the spleen of both mucosally primed animals

  19. Detection of urban heat island in Ankara, Turkey

    International Nuclear Information System (INIS)

    Ankara is the second largest city in Turkey after Istanbul, and the rate of population increase and urbanization are quite high. In this study, the effects of urbanization on temperature variation due to urbanization in Ankara were investigated. The intensities of urban heat island (UHI) for long and short term were analyzed. Analysis of both long- and short-term data revealed that there is a significant increase in the intensity of UHI (AT(u-r)) in winter during the period analyzed. Analysis of data collected for period of October 2001-September 2002 shows that intensity of maximum UHI is in February. In this month, positive UHI was observed in 26 nights and on all these days wind speed was less than 0.5ms.1. UHI is positive in all seasons and frequency and intensity of UHI in winter are higher than in the other seasons. This characteristic makes Ankara different from other temperate latitude cities

  20. Subtype C gp140 Vaccine Boosts Immune Responses Primed by the South African AIDS Vaccine Initiative DNA-C2 and MVA-C HIV Vaccines after More than a 2-Year Gap.

    Science.gov (United States)

    Gray, Glenda E; Mayer, Kenneth H; Elizaga, Marnie L; Bekker, Linda-Gail; Allen, Mary; Morris, Lynn; Montefiori, David; De Rosa, Stephen C; Sato, Alicia; Gu, Niya; Tomaras, Georgia D; Tucker, Timothy; Barnett, Susan W; Mkhize, Nonhlanhla N; Shen, Xiaoying; Downing, Katrina; Williamson, Carolyn; Pensiero, Michael; Corey, Lawrence; Williamson, Anna-Lise

    2016-06-01

    A phase I safety and immunogenicity study investigated South African AIDS Vaccine Initiative (SAAVI) HIV-1 subtype C (HIV-1C) DNA vaccine encoding Gag-RT-Tat-Nef and gp150, boosted with modified vaccinia Ankara (MVA) expressing matched antigens. Following the finding of partial protective efficacy in the RV144 HIV vaccine efficacy trial, a protein boost with HIV-1 subtype C V2-deleted gp140 with MF59 was added to the regimen. A total of 48 participants (12 U.S. participants and 36 Republic of South Africa [RSA] participants) were randomized to receive 3 intramuscular (i.m.) doses of SAAVI DNA-C2 of 4 mg (months 0, 1, and 2) and 2 i.m. doses of SAAVI MVA-C of 1.45 × 10(9) PFU (months 4 and 5) (n = 40) or of a placebo (n = 8). Approximately 2 years after vaccination, 27 participants were rerandomized to receive gp140/MF59 at 100 μg or placebo, as 2 i.m. injections, 3 months apart. The vaccine regimen was safe and well tolerated. After the DNA-MVA regimen, CD4(+) T-cell and CD8(+) T-cell responses occurred in 74% and 32% of the participants, respectively. The protein boost increased CD4(+) T-cell responses to 87% of the subjects. All participants developed tier 1 HIV-1C neutralizing antibody responses as well as durable Env binding antibodies that recognized linear V3 and C5 peptides. The HIV-1 subtype C DNA-MVA vaccine regimen showed promising cellular immunogenicity. Boosting with gp140/MF59 enhanced levels of binding and neutralizing antibodies as well as CD4(+) T-cell responses to HIV-1 envelope. (This study has been registered at ClinicalTrials.gov under registration no. NCT00574600 and NCT01423825.). PMID:27098021

  1. Blood Parasites of Wild Pigeons in Ankara District

    OpenAIRE

    GICIK, Yunus; ARSLAN, M. Özkan

    2001-01-01

    A total of 200 wild pigeons, 82 young and 118 adults, captured from 12 localities in Ankara District, were examined for blood parasites. Haemoproteus columbae was found in 57% (114/200) of the pigeons examined, and no other blood parasites were detected.

  2. Caracterización del Poliuretano Modificado con Sacarosa (PSU)

    OpenAIRE

    Adolfo Brown; Bárbara Gregori; Gastón García; Livan Alba; Cristina Gastón

    2005-01-01

    El presente estudio se refiere a la caracterización del poliuretano modificado con sacarosa (PSU) con vistas a evaluar sus posibles aplicaciones como encapsulante y en sistemas de liberación controlada (SLC). Para esto, el producto fue sometido a pruebas de toxicidad aguda oral y se utilizó la Calorimetría Diferencial de Barrido (DSC) como herramienta eficaz en la evaluación de parámetros físico químicos que inciden en sus propiedades como adhesivo y formador de películas. Los resultados obte...

  3. Enhanced cell surface expression, immunogenicity and genetic stability resulting from a spontaneous truncation of HIV Env expressed by a recombinant MVA

    International Nuclear Information System (INIS)

    During propagation of modified vaccinia virus Ankara (MVA) encoding HIV 89.6 Env, a few viral foci stained very prominently. Virus cloned from such foci replicated to higher titers than the parent and displayed enhanced genetic stability on passage. Sequence analysis showed a single nucleotide deletion in the 89.6 env gene of the mutant that caused a frame shift and truncation of 115 amino acids from the cytoplasmic domain. The truncated Env was more highly expressed on the cell surface, induced higher antibody responses than the full-length Env, reacted with HIV neutralizing monoclonal antibodies and mediated CD4/co-receptor-dependent fusion. Intramuscular (IM), intradermal (ID) needleless, and intrarectal (IR) catheter inoculations gave comparable serum IgG responses. However, intraoral (IO) needleless injector route gave the highest IgA in lung washings and IR gave the highest IgA and IgG responses in fecal extracts. Induction of CTL responses in the spleens of individual mice as assayed by intracellular cytokine staining was similar with both the full-length and truncated Env constructs. Induction of acute and memory CTL in the spleens of mice immunized with the truncated Env construct by ID, IO, and IR routes was comparable and higher than by the IM route, but only the IR route induced CTL in the gut-associated lymphoid tissue. Thus, truncation of Env enhanced genetic stability as well as serum and mucosal antibody responses, suggesting the desirability of a similar modification in MVA-based candidate HIV vaccines

  4. Caracterización del Poliuretano Modificado con Sacarosa (PSU

    Directory of Open Access Journals (Sweden)

    Adolfo Brown

    2005-01-01

    Full Text Available El presente estudio se refiere a la caracterización del poliuretano modificado con sacarosa (PSU con vistas a evaluar sus posibles aplicaciones como encapsulante y en sistemas de liberación controlada (SLC. Para esto, el producto fue sometido a pruebas de toxicidad aguda oral y se utilizó la Calorimetría Diferencial de Barrido (DSC como herramienta eficaz en la evaluación de parámetros físico químicos que inciden en sus propiedades como adhesivo y formador de películas. Los resultados obtenidos nos permitieron sugerir las modificaciones necesarias para lograr los objetivos propuestos.

  5. Partially randomized, non-blinded trial of DNA and MVA therapeutic vaccines based on hepatitis B virus surface protein for chronic HBV infection.

    Directory of Open Access Journals (Sweden)

    James S Cavenaugh

    Full Text Available BACKGROUND: Chronic HBV infects 350 million people causing cancer and liver failure. We aimed to assess the safety and efficacy of plasmid DNA (pSG2.HBs vaccine, followed by recombinant modified vaccinia virus Ankara (MVA.HBs, encoding the surface antigen of HBV as therapy for chronic HBV. A secondary goal was to characterize the immune responses. METHODS: Firstly 32 HBV e antigen negative (eAg(- participants were randomly assigned to one of four groups: to receive vaccines alone, lamivudine (3TC alone, both, or neither. Later 16 eAg(+ volunteers in two groups received either 3TC alone or both 3TC and vaccines. Finally, 12 eAg(- and 12 eAg(+ subjects were enrolled into higher-dose treatment groups. Healthy but chronically HBV-infected males between the ages of 15-25 who lived in the western part of The Gambia were eligible. Participants in some groups received 1 mg or 2 mg of pSG2.HBs intramuscularly twice followed by 5×10(7 pfu or 1.5×10(8 pfu of MVA.HBs intradermally at 3-weekly intervals with or without concomitant 3TC for 11-14 weeks. Intradermal rabies vaccine was administered to a negative control group. Safety was assessed clinically and biochemically. The primary measure of efficacy was a quantitative PCR assay of plasma HBV. Immunity was assessed by IFN-γ ELISpot and intracellular cytokine staining. RESULTS: Mild local and systemic adverse events were observed following the vaccines. A small shiny scar was observed in some cases after MVA.HBs. There were no significant changes in AST or ALT. HBeAg was lost in one participant in the higher-dose group. As expected, the 3TC therapy reduced viraemia levels during therapy, but the prime-boost vaccine regimen did not reduce the viraemia. The immune responses were variable. The majority of IFN-γ was made by antigen non-specific CD16(+ cells (both CD3(+ and CD3(-. CONCLUSIONS: The vaccines were well tolerated but did not control HBV infection. TRIAL REGISTRATION: ISRCTN ISRCTN67270384.

  6. Ultrasound for critical care physicians: hypotension after a MVA

    Directory of Open Access Journals (Sweden)

    Schmitz E

    2014-03-01

    Full Text Available No abstract available. Article truncated after first page. A 25 year old woman was a restrained driver in a rollover motor vehicle accident (MVA and suffered a C5-C6 fracture-dislocation with spinal cord injury. She was lucid and able to follow commands and could move her upper extremities but not her lower extremities. She was given approximately 6 liters of fluid but required vasopressors to maintain her blood pressure. Initial ECG revealed a normal sinus rhythm without significant ST changes (Figure 1. Upon initial evaluation her blood pressure was low. Bedside ultrasound of the left anterior second intercostal space revealed a sliding lung sign and a 4 chamber view of her heart was performed (Figure 2. Which of the following is the most likely cause of her hypotension? 1. Blunt cardiac injury; 2. Intravascular volume depletion; 3. Neurogenic stunned myocardium; 4. Pericardial tamponade; 5. Pneumothorax ...

  7. A Modified Vaccinia Ankara Virus (MVA) Vaccine Expressing African Horse Sickness Virus (AHSV) VP2 Protects Against AHSV Challenge in an IFNAR −/− Mouse Model

    OpenAIRE

    Castillo-Olivares, Javier; Calvo-Pinilla, Eva; Casanova, Isabel; Bachanek-Bankowska, Katarzyna; Chiam, Rachael; Maan, Sushila; Nieto, Jose Maria; Ortego, Javier; Mertens, Peter Paul Clement

    2011-01-01

    African horse sickness (AHS) is a lethal viral disease of equids, which is transmitted by Culicoides midges that become infected after biting a viraemic host. The use of live attenuated vaccines has been vital for the control of this disease in endemic regions. However, there are safety concerns over their use in non-endemic countries. Research efforts over the last two decades have therefore focused on developing alternative vaccines based on recombinant baculovirus or live viral vectors exp...

  8. Towards a universal vaccine for avian influenza: protective efficacy of modified Vaccinia virus Ankara and Adenovirus vaccines expressing conserved influenza antigens in chickens challenged with low pathogenic avian influenza virus.

    Science.gov (United States)

    Boyd, Amy C; Ruiz-Hernandez, Raul; Peroval, Marylene Y; Carson, Connor; Balkissoon, Devanand; Staines, Karen; Turner, Alison V; Hill, Adrian V S; Gilbert, Sarah C; Butter, Colin

    2013-01-11

    Current vaccines targeting surface proteins can drive antigenic variation resulting either in the emergence of more highly pathogenic viruses or of antigenically distinct viruses that escape control by vaccination and thereby persist in the host population. Influenza vaccines typically target the highly mutable surface proteins and do not provide protection against heterologous challenge. Vaccines which induce immune responses against conserved influenza epitopes may confer protection against heterologous challenge. We report here the results of vaccination with recombinant modified Vaccinia virus Ankara (MVA) and Adenovirus (Ad) expressing a fusion construct of nucleoprotein and matrix protein (NP+M1). Prime and boost vaccination regimes were trialled in different ages of chicken and were found to be safe and immunogenic. Interferon-γ (IFN-γ) ELISpot was used to assess the cellular immune response post secondary vaccination. In ovo Ad prime followed by a 4 week post hatch MVA boost was identified as the most immunogenic regime in one outbred and two inbred lines of chicken. Following vaccination, one inbred line (C15I) was challenged with low pathogenic avian influenza (LPAI) H7N7 (A/Turkey/England/1977). Birds receiving a primary vaccination with Ad-NP+M1 and a secondary vaccination with MVA-NP+M1 exhibited reduced cloacal shedding as measured by plaque assay at 7 days post infection compared with birds vaccinated with recombinant viruses containing irrelevant antigen. This preliminary indication of efficacy demonstrates proof of concept in birds; induction of T cell responses in chickens by viral vectors containing internal influenza antigens may be a productive strategy for the development of vaccines to induce heterologous protection against influenza in poultry. PMID:23200938

  9. A Novel AT-Rich DNA Recognition Mechanism for Bacterial Xenogeneic Silencer MvaT.

    Directory of Open Access Journals (Sweden)

    Pengfei Ding

    2015-06-01

    Full Text Available Bacterial xenogeneic silencing proteins selectively bind to and silence expression from many AT rich regions of the chromosome. They serve as master regulators of horizontally acquired DNA, including a large number of virulence genes. To date, three distinct families of xenogeneic silencers have been identified: H-NS of Proteobacteria, Lsr2 of the Actinomycetes, and MvaT of Pseudomonas sp. Although H-NS and Lsr2 family proteins are structurally different, they all recognize the AT-rich DNA minor groove through a common AT-hook-like motif, which is absent in the MvaT family. Thus, the DNA binding mechanism of MvaT has not been determined. Here, we report the characteristics of DNA sequences targeted by MvaT with protein binding microarrays, which indicates that MvaT prefers binding flexible DNA sequences with multiple TpA steps. We demonstrate that there are clear differences in sequence preferences between MvaT and the other two xenogeneic silencer families. We also determined the structure of the DNA-binding domain of MvaT in complex with a high affinity DNA dodecamer using solution NMR. This is the first experimental structure of a xenogeneic silencer in complex with DNA, which reveals that MvaT recognizes the AT-rich DNA both through base readout by an "AT-pincer" motif inserted into the minor groove and through shape readout by multiple lysine side chains interacting with the DNA sugar-phosphate backbone. Mutations of key MvaT residues for DNA binding confirm their importance with both in vitro and in vivo assays. This novel DNA binding mode enables MvaT to better tolerate GC-base pair interruptions in the binding site and less prefer A tract DNA when compared to H-NS and Lsr2. Comparison of MvaT with other bacterial xenogeneic silencers provides a clear picture that nature has evolved unique solutions for different bacterial genera to distinguish foreign from self DNA.

  10. Urban and Indoor Weak Signal Tracking Using an Array Tracker with MVA and Nonlinear Filtering

    OpenAIRE

    Jicheng Ding; Guoli Zhang; Lin Zhao

    2014-01-01

    We focus on the need for weak GPS signal tracking technique at a receiver powered on in urban or indoor environment; the tracking loop is unlocked and data bit edge position is unknown. A modified Viterbi algorithm (MVA) based on dynamic programming is developed and it is applied to GPS bit synchronization to improve bit edge position detection probability. Meanwhile, two combination carrier tracking schemes based on central difference Kalman filter (CDKF) and MVA module are designed for trac...

  11. An Investigation on Satisfaction of Domestic Tourist from Ankara Shopping Fest under the Tourism of Festival

    Directory of Open Access Journals (Sweden)

    Ahmet TAYFUN

    2013-06-01

    Full Text Available Concentration of Tourism mostly in coastal of Turkey, in certain centers and in summer season stands out as a major problem. A tourism mobility that, spread over 12 months, and including inner zones is emphasized also in 2023 Tourism Strategy of Turkey. Therefore, increasing the diversity of tourism such as festivals, which started to increase number of events. Hence, investigated in this study, satisfaction levels from Ankara Shopping Fest of domestic tourists, who came to Ankara during "Ankara Shopping Fest" which, organized in Ankara on 8 June to 1 July 2012 period. Descriptive research method used in the study. The aims in this study, determination of the views of local tourists about Ankara Shopping Fest and to investigate the satisfaction from the festival. 336 domestic tourists as visitors during the festival in Ankara were the sample of research. Questionnaire technique was used as a data collection technique. The questionnaire consisted of three parts, the first section was demographic variables. The second section was located expressions for Ankara Shopping Fest. Some statical analysis made to data in the result of research. These analyses were frequecy analysis, t tests and ANOVA tests. As a result of the t-tests and ANOVA tests significant differences were found between gender, age, level of income and civil staus of domestic tourists who came to Ankara during the festival.

  12. 生防假单胞菌2P24中mvaT和mvaV基因对PcoI/PcoR群体感应系统的调控作用%MvaT and MvaV transcriptionally regulate PcoI/PcoR quorum-sensing system in Pseudomonas fluorescens 2P24

    Institute of Scientific and Technical Information of China (English)

    吴小刚; 魏亚蕊; 刘九成; 张力群

    2012-01-01

    [Objective] Pseudomonas fluorescens 2P24 is an effective biocontrol agent for soil-borne plant diseases caused by microbial pathogens. The PcoI/PcoR quorum-sensing system, which influences the colonization ability of 2P24 on wheat rhizosphere, is an important factor for disease suppression. In this study we performed random mutagenesis to screen novel regulators of the pcol gene, a biosynthase gene responsible for iV-acyl-homoserine lactone (AHL) production. [ Methods ] A gacA gene mutant carrying a pcoI-lacZ fusion was employed as the reporter strain and subjected to a random mini-Tn5 insertion mutagenesis. Expression of pcol kept at a low level under the gacA- negative background. The Tn5-mutants with increased pcol transcription were selected. [Results] Two mutants with significantly increased pcol expression were identified from ~ 10000 Tn5-inserted colonies. The interrupted locus in the mutants was identified as the mvaT gene, a global regulator belonging to the H-NS family. A homolog of the mvaT gene, named mvaV, was also found in the genome draft sequence of 2P24. Genetic inactivation of mvaT or mvaV gene resulted in increased transcription of pcol and the production of AHL molecules. Further qutitification by HPLC showed that the 2,4-diacetylphloroglucinol (2, 4-DAPG) levels in culture supernatant of the mvaT and mvaV mutants were significantly lower than that of the wild type strain. Furthermore, the mvaT or mvaV mutation drastically improved biofilm formation in 2P24. [Conclusion] MvaT and MvaV may function as an important regulatory complex controlling biocontrol capacity of P. fluorescens 2P24.%[目的] 自小麦全蚀病自然衰退土壤分离得到的荧光假单胞菌(Pseudomonas fluorescens)2P24,可防治多种由植物病原菌引起的土传病害.菌株2P24具有群体感应(quorum-sensing,QS)系统PcoI/PcoR,该系统影响生防菌2P24生物膜的形成以及其在小麦根围的定殖能力,从而影响2P24的生防能力.本文利用遗

  13. Barriers to sustainable MVA supply in Ghana: challenges for the low-volume, low-income providers.

    Science.gov (United States)

    Graff, Maura; Amoyaw, Dora A

    2009-12-01

    Multiple studies have demonstrated that manual vacuum aspiration (MVA) is ideal for surgical uterine evacuation in low-resource settings such as Ghana, but developing a sustainable supply to MVA has been challenging. In 2007 a situational analysis was conducted in Ghana to identify barriers to sustainable MVA supply. Information about MVA availability was gathered in seven regions of Ghana and obtained through background literature, unpublished data and reports, and 70 informational interviews with stakeholders involved with MVA policy, manufactufing, procurement, distribution, supply, training, and provision. The findings revealed that despite consensus about the dire need for MVA in Ghana, developing sustainable access to MVA instruments has proven difficult. In the public and the private health sectors, procuring MVA equipment has been particularly challenging for low-income, low-volume service providers. Research findings yielded ten recommendations for improving sustainable access to MVA, including the implementation of a revolving purchase mechanismn for health provider associations, such as the Ghana Registered Midwives Association. PMID:20690275

  14. Behzat Ç. An Ankara Detective: An Audience Perception Analysis

    Directory of Open Access Journals (Sweden)

    Tülay Şeker

    2011-09-01

    Full Text Available This study was proposed to reveal how “Behzat Ç. An Ankara Detective” serial is perceived among audiences. The research was done by 11 participants and by means of deep engagements audience notions devoted to reception aesthetic were examined. It was confirmed that audiences were highly sensitive to messages. We acquired that socio-demographic variables differ from presented content in terms of resistance. While sense production related to social reality was closer to mass media reality among the youth, elder groups and educateds producted senses that did not coincide with tendency of serial. During the engagements, Behzat Ç. was characterized as a political person and the idea that certain political parties and communities were targeted became prominent. It was determined that the audience of Behzat Ç. interprets each message according to his or her intellectual and ideological framework with an active and sensitive eye.

  15. The Thermal Performance of a 1.5 MVA HTS Generator

    Science.gov (United States)

    Urbahn, J. A.; Ackermann, R. A.; Huang, X.; Laskaris, E. T.; Sivasubramaniam, K.; Steinbach, A.

    2004-06-01

    A 1.5-MVA high temperature superconducting ( HTS ) generator of novel design has been designed, built and successfully tested by the General Electric Company. The 1.5- MVA generator has served as the engineering prototype for a much larger 100-MVA beta unit now under design. The HTS coil in the 1.5 - MVA demonstrator is designed to operate in the range of 20-40 K and is cooled with a closed-cycle helium refrigeration system employing GM type cryocoolers. This paper will discuss the calculation of the thermal loads to the rotor from all anticipated sources. These sources include conduction losses through the coil suspension system, radiative heat loads to the cold-system components, residual gas conduction losses, helium-transfer coupling losses and lead losses. These predicted losses were compared to those measured during actual electrical testing of the rotor at 3600 RPM in order to validate the predictive calculations employed for the 100 MVA machine.

  16. Organismos modificados genéticamente en la alimentación humana

    OpenAIRE

    Barros Fernández, Paula

    2014-01-01

    El presente trabajo trata la controversia del tema de los organismos modificados genéticamente (OMG). Se mencionan los beneficios que aporta la ingeniería genética y también los principales riesgos y preocupaciones existentes en torno al consumo de los alimentos modificados genéticamente, reportando casos de estudios que así lo constatan.Se tratan temas como seguridad alimentaria, legislación y normativas de etiquetado de estos nuevos alimentos, señalando su relación con la salud. Además, se ...

  17. A validade e reprodutibilidade do protocolo run-bike-run modificado

    OpenAIRE

    Cavaleiro, João Pedro dos Santos

    2011-01-01

    Este estudo pretende verificar a validade e reprodutibilidade do protocolo Run-Bike- Run (RBR) modificado, em 7 triatletas de distância sprint (SD), 8 distância olímpica (OD) e 8 longa distância (LD) não elite. Foram efectuados 4 testes: 1) progressivo de corrida (TPC), 2-3) Dois RBR modificado e 3) contra-relógio (30 minutos ciclismo + 20 minutos corrida). Foram encontradas diferenças entre OD e LD no VO2máx e o LV como percentagem do VO2máx do TPC (p

  18. Temperaturas de fabrico e compactação de misturas betuminosas com betumes modificados

    OpenAIRE

    Santos, André Filipe Rodrigues

    2011-01-01

    Os betumes modificados, contrariamente aos puros, são conhecidos por terem um comportamento reológico não-Newtoniano do tipo “shear-thinning”. Para estes a viscosidade depende da temperatura, da taxa de corte, do agente modificador e da sua percentagem. Devido à alteração do comportamento reológico, a determinação das temperaturas de fabrico e compactação dos betumes modificados exige metodologias diferentes das utilizadas para betumes puros. Destas metodologias resultam temperaturas muito el...

  19. Jurados ciudadanos y organismos genéticamente modificados

    Directory of Open Access Journals (Sweden)

    Luque, Emilio

    2005-04-01

    Full Text Available Apolitical sociology of food must look into the processes whereby regulatory decisions on food are made, and also into the democratic potential of their alternatives. Five "experiments in democracy" are described, in which a promising deliberative device has been used: citizens' juries. Indian and Brazilian peasants, on the one hand, and British consumers in the other took part in them, and they focused on Genetically Modified Organisms. These processes, whose defining trait is granting jurors access to expert witnesses presented by all stakeholders in the controversy, show the impressive ability of ordinary citizens to articulate their analysis of complex issues, a reassessment of risks, and a recontextualization of the use of GMOs. At any rate, deliberative democracy is not a magic bullet for the epistemic and political crisis that underlies food crisis; instead, it points at a paradigm change towards an experimental democratic polity in which the instances of representation of the public and publics are multiplied.

    Una sociología política de la alimentación debe analizar los procesos a través de los cuales se alcanzan las decisiones de regulación y control alimentario y examinar el potencial de sus alternativas. Se describen aquí cinco experimentos democráticos en los que se ha empleado uno de los dispositivos deliberativos más prometedores en condiciones de alta complejidad cognitiva, los llamados jurados ciudadanos, con la participación de campesinos hindúes y brasileños y consumidores británicos, y centrados en los Organismos Genéticamente Modificados. Estos procesos, caracterizados por el acceso de los miembros del jurado a testigos expertos presentados por los distintos participantes en la controversia, muestran la enorme capacidad de los ciudadanos "de a pie" para articular el análisis de un problema complejo, reevaluar sus riesgos y recontextualizar el uso de los OGM. En todo caso, la democracia deliberativa no es

  20. Conceptual Design of a Single Phase 33 MVA HTS Transformer with a Tertiary Winding

    International Nuclear Information System (INIS)

    We have proposed a 3 phase, 100 MVA, 154 kV class HTS transformer substituting for a 60 MVA conventional transformer. The power transformer of 154 kV class has a tertiary winding besides primary and secondary windings. So the HTS transformer should have the 3rd superconducting winding. In this paper, we designed conceptually the structure of the superconducting windings of a single phase 33 MVA transformer. The electrical characteristics of the HTS transformer such as % impedance and AC loss vary with the arrangement of the windings and gaps between windings. We analyzed the effects of the winding parameters, evaluated the cost of each design, and proposed a suitable HTS transformer model for future power distribution system.

  1. Ankara Historic City Centre Restoration Site Conservation Plan, its Characteristics, and Rationales for its Revocation

    Directory of Open Access Journals (Sweden)

    Mehmet Tuncer

    2013-12-01

    Full Text Available This is a comprehensive evaluation of the 1: 5000 Ankara Historic City Centre Restoration Site Conservation Master Development Plan and the 1: 1000 Conservation Implementation Plans, which have become a matter of litigation against the Ankara Metropolitan Municipality, filed by various non-governmental organizations, universities, the Union of Chambers of Turkish Architects and Engineers (TMMOB and public institutions; in terms of the environment, transportation and traffic as regards the entirety of the planned location, and the integrity of the plan, based on conservation-reclamation planning essentials and public interest. Thus the Ulus Ankara Historic City Centre Restoration Site Conservation Master Development and Implementation Plan and Plan Notes as ratified by the Ankara Metropolitan Municipality and Conservation Board, are examined in this context in terms of restoration site, protected site, conservation site, interaction and transition sites and historic and cultural properties.

  2. Construction of recombinant modified vaccinia virus Ankara with Ag85A and ESAT-6 gene and examination of their immunogenicity in mice%重组结核抗原痘苗病毒Ankara株的构建及其免疫原性研究

    Institute of Scientific and Technical Information of China (English)

    楼觉人; 张群; 朱琳

    2008-01-01

    目的 构建5种不同类型的表达结核杆菌特异抗原的重组痘苗病毒,并研究其特异免疫原性.方法 运用同源重组技术将含结核分泌抗原Ag85A和ESAT-6的基因片段插入痘苗病毒表达质粒p18中.重组质粒导入痘苗病毒Ankara(MVA)后构建重组痘苗病毒,经筛选和Western blot鉴定,得到5个种类的带有结核抗原基因的重组病毒.用构建的5种重组病毒免疫小鼠,MTT法检测免疫后小鼠脾淋巴细胞对特异结核抗原的增殖反应;ELISA检测小鼠脾淋巴细胞培养上清液中IFN-γ的含量;结核菌素纯蛋白衍化物(PPD)皮内试验以检测重组病毒引发的针对结核抗原的特异细胞免疫应答.结果 构建的5种蘑组病毒介导的细胞表达产物经Western blot鉴定确认相对分子质量与结核抗原一致.免疫小鼠两次后,5种重组病毒免疫组脾淋巴细胞体外与Ag85A-ESAT-6融合蛋白共培养后表现出明显的增殖活性(P<0.01),培养上清液中IFN-γ的浓度均较同组细胞经生理盐水刺激明显增高(P<0.05);与空痘苗病毒或生理盐水免疫后小鼠相比,5种重组MVA免疫组脾淋巴细胞与AgB5A.ESAT-6融合蛋白共培养后同样表现出明显的增殖活性(P<0.01),与Ag85A-ESAT-6融合蛋白共培养的细胞上清液中IFN-γ的浓度均升高(P<0.01).与空痘苗病毒或生理盐水免疫后小鼠相比,5种重组MVA免疫组小鼠对PPD都表现出显著的迟发型超敏反应应答(P<0.05).结论 成功构建了5种不同类型的表达结核杆菌抗原的重组痘苗病毒疫苗,其免疫小鼠后可激发针对结核杆菌抗原的特异性细胞免疫.%Objective To construct five types of recombinant modified vaccinia virus Ankara (MVA) carrying genes encoding antigen 85A (Ag85A), early secretory antigenic target (ESAT-6) or IL-2 and to investigate the immunogenicity of these recombinant MVA in mice. Methods The genes encoding Ag85A and ESAT-6 were amplified by PCR from Mycobacterium

  3. Landscaping to Reduce Traffic Noise Problem in Cities: Ankara Case

    Directory of Open Access Journals (Sweden)

    M. E. Yazgan

    2007-05-01

    Full Text Available Cities are suffering under the effects of unexpected population growth due to immigration to metropolitan areas. Thus, urban environments are heavily loaded so that the existing infrastructure becomes insufficient and new services are needed. Besides the technological developments and changes in the community, urban environments are affected negatively due to transporation and traffic problems. On one hand, highways built to shorten the travel period and the bypasses to speed transportation time and capacity brings comfort in service functions, on the other hand, creates numerous negative effects like functional, visual and auditory damages in urban environments. In this article, noise, sources of noise and other related terminology were identified then, negative effects of noise in urban areas and the precautions that has to be taken were determined. At the final stage, the role of plant material in reducing the effects of noise was discussed and the plantation design for an effective noise control and plant species that could be used to eleminate or reduce noise for Ankara city were proposed.

  4. Archaeometric exploration at Akköprü in Ankara

    Directory of Open Access Journals (Sweden)

    Ali Akın Akyol

    2013-01-01

    Full Text Available The archaeometric exploration was conducted by surveying and sampling studies on the stone, and mortar samples from Akköprü in Ankara. Spot salt test and conductometric analysis were applied to get water soluble salt content of stone samples. The aggregate and binder part of the mortars were determined by the analyses of acidic aggregate & binder, aggregate granulometry, thin section optic microscopy and X-ray diffraction. In addition, the physical conditions of the stones were determined by some physical tests. The results of archaeometrical data showed that the constructive stone material was mainly andesite besides ignimbrite and dacite, and the original binder material was lime mortar. The cement content of the binder of mortars reveals the recent interventions on Akköprü. In the light of petrographic studies it was understood that the source of the original constructive raw material reflects the local formation. The high soluble salt content and the bad physical conditions of some stones also showed that they are undergoing a decomposition process of different degrees.

  5. Lottery Houses (İş Bank in Ankara

    Directory of Open Access Journals (Sweden)

    Umut Şumnu

    2014-06-01

    Full Text Available The importance of (savings incentive lottery houses that emerged in the late 1930’s and served for nearly fourty years as tools in the formation and development of Turkey’s accommodation/habitation culture has been largely ignored in architectural history. The mainstream historic accounts based mostly on public buildings or a minimum of civil architectural constructions that may only serve an ideological “benefit” for the dominant narrative, have disregarded the field of lottery houses. Lottery houses have stayed/keptout of the traditional narrative based on the accommodation culture proposed by the state. Thus, this study aims to investigate the lottery houses process in Turkey, and look at the development of that process, as regards particularly the buildings produced by İş Bank in Ankara. İş Bank was chosen as the subject, as it was the originator of the savings incentive lottery house plans and it has emerged as one of the most well-established and powerful institutions in the process, producing architectural constructions distinctive both in terms of quality and quantity.

  6. HEALTH BEHAVIOURS OF THE PERSONNEL OF PRIMARY SCHOOLS IN ANKARA

    Directory of Open Access Journals (Sweden)

    Serife AK

    2006-04-01

    Full Text Available The main purpose of study is to define the health behaviour profile of the personnel of primary schools as well as to define the topics to be given priority in health education activities. The research population consisted of all personnel of 564 primary schools in Ankara province. The study group (30 schools was estimated by cluster sampling method. The Health Behaviours Questionnaire, which consists of 47 items on health behaviours and demographic characteristics, was used for data collection. In the study, 849 school workers (67,5% female were interviewed. Teachers constituted the largest group (82,3% while the cleaning staff (7.0%, school administrators (6.3%, and other personnel (4.4% were sharing the rest in small proportions. Of the study group 41,7 % are cigarette smokers and of them 67.3% smoke every time and 11,9% sometime in school. Very few school personnel (14,8% do physical exercise regularly. Majority of school personnel (71,3% stated that they brush their teeth at least twice a day regularly. However, only 23,7% of school personnel go to have regular dental control. All results were discussed in details considering the effects of health behaviours of school personnel on students, and some recommendations were developed for health education activities in schools. [TAF Prev Med Bull 2006; 5(2.000: 83-93

  7. Characterization and source determination of air pollution in Ankara

    International Nuclear Information System (INIS)

    The sources of air pollution in Ankara and the effect of these sources on rainwater composition were studied by collecting rainwater and aerosol samples over a one year period in 1988. The rain water was not acidic owing to high concentrations of alkaline soil particles in the atmosphere. However, the concentrations of acid forming ions, such as SO42- and NO3-, were higher than concentrations expected in a typical urban atmosphere. Most of the SO42- in rain water was in the form of CaSO4. The ions most efficiently scavenged from the atmosphere were found to be SO42- and Ca2+. Atomspheric concentrations of approximately thirty elements indicated that road dust, motor vehicles, coal and oil combustion are the main components of aerosols over the city. Elements which have origins in industrial processes were not enriched substantially and do not show seasonal dependence, whereas elements which originate from coal combustion for residential heating are highly enriched and have higher concentrations in the winter. Road dust was enriched in Pb and Br and does not show characteristic soil composition. (author). 8 refs, 5 figs, 7 tabs

  8. Risk-Informed Monitoring, Verification and Accounting (RI-MVA). An NRAP White Paper Documenting Methods and a Demonstration Model for Risk-Informed MVA System Design and Operations in Geologic Carbon Sequestration

    Energy Technology Data Exchange (ETDEWEB)

    Unwin, Stephen D.; Sadovsky, Artyom; Sullivan, E. C.; Anderson, Richard M.

    2011-09-30

    This white paper accompanies a demonstration model that implements methods for the risk-informed design of monitoring, verification and accounting (RI-MVA) systems in geologic carbon sequestration projects. The intent is that this model will ultimately be integrated with, or interfaced with, the National Risk Assessment Partnership (NRAP) integrated assessment model (IAM). The RI-MVA methods described here apply optimization techniques in the analytical environment of NRAP risk profiles to allow systematic identification and comparison of the risk and cost attributes of MVA design options.

  9. Daily Ambrosia Pollen Concentration in the Air of Ankara,Turkey (1990-1999)

    Institute of Scientific and Technical Information of China (English)

    Ayse KAPLAN; Nazmiye SAKIYAN; N Münevver PINAR

    2003-01-01

    The airborne ragweed pollen spectrum was investigated in the air of Ankara, Turkey for aperiod of ten years (1990-1999) using a Burkard seven-day volumetric recording trap. In our study period,long distance transported Ambrosia pollen has been registered. Daily pollen levels varied from low to highin Burge's system. In last three years, the pollen concentration of Ambrosia showed a clear increasingtendency. Our results prove that ragweed pollen may be an important threat for ragweed sensitive patientsin Ankara city in near future.

  10. Root and crown rot and wilt of tomatoes caused by fungal diseases in Ankara province.

    OpenAIRE

    Ozan, S.; Maden, S.

    2009-01-01

    This study was conducted in Ayaş, Beypazarı and Nallıhan districts of Ankara province during May-November 2003-2004 in order to detect fungal pathogens of tomato, and to find out the incidence, prevalence and occurrence times of these pathogens. It is detected that wilt and root rot pathogens causes important yield losses in tomato fields in Ankara. The incidences of the fungi isolated roots and crowns were 17.2 %, 0.96 %, 0.32 %, 0.91 %, 2.95 %,% 0.52 for Pyrenochaeta lycopersici, Fusa...

  11. EFEITO DE FUNGOS MVA NATIVOS E INTRODUZIDOS NO RENDIMENTO DE CORNICHÃO

    Directory of Open Access Journals (Sweden)

    Antônio Carlos dos Santos Pessoa

    1994-01-01

    Full Text Available RESUMO Com o objetivo de avaliar o efeito de fMVA nativos, Glomus clarum e Glomus etunicatum, na absorção de fósforo (P e produção de massa seca (MS pelo cornichão (Lotus comiculatus L. em condições naturais e modificadas pela calagem e verificar a compatibilidade entre as espécies introduzidas e os fMVA nativos ocorrentes no solo, conduziu-se um experimento em casa de vegetação, em solo Podzólico Vermelho Amarelo (Hapludalf. Os tratamentos constituiram-se de fatorial 5x2x2, ou seja, cinco níveis de combinação com fMVA (Glomus clarinn, Glomus etunicatum, fungos nativos, mistura de fungos nativos mais as duas espécies de Glomus e sem inoculação com fMVA; com pH 4,8 e 6,0 e duas doses de fósforo 30 e 90mg de P2O5/kg de solo, com três repetições, no delineamento inteiramente casualizado. Os maiores rendimentos de MS e acumulação de P foram obtidos nos tratamentos que receberam a dose mais alta de P e calagem para pH 6,0. O fMVA Glomus clarum foi efetivo e eficiente em suprir P às plantas de cornichão quando cultivado em solo com baixa disponibilidade de P (5,5ppm ex-trator Mehlich e em condições de pH baixo (pH 4.5, aumentando a produção de MS a acumulação de P, mas quando em condição intermediária de P (15ppm extrator Mehlich e pH baixo (pH 4,5 os fungos introduzidos não diferiram da testemunha e fMVA nativos para a produção de MS, mas a acumulação de P foi maior nos tratamentos que receberam Glomus clarum. Em condições de pH moderadamente baixo (pH 5,5 e disponibilidade intermediária de P os fungos introduzidos foram superiores à testemunha e fMVA nativos para a produção de MS. Os tratamentos com os maiores níveis de taxa de colonização micorrízica corresponderam aos de maiores rendimentos de MS, acumulação de P e ao maior número de esporos encontrados após o cultivo, evidenciando que a introdução de fMVA pode ser viável quando as espécies nativas não são eficientes para a cultura ou

  12. Thermal stress analysis and design of the stator of a 300 MVA superconducting generator

    International Nuclear Information System (INIS)

    This paper presents the results for an axisymmetric finite element analysis of the stator armature and flux shield of a 300 MVA superconducting generator subject to manufacturing and operational thermal events. These machines typically will have large air gap armatures, departing from conventional generator experience. The mechanical design of such an armature to withstand operational loads is a new area. Key stresses are determined in the structure, especially interlaminar tensile stresses in the insulating skins and conductors, and compressive and tensile stresses along the axes of the armature conductors. Recommendations are made for further investigations. This work was performed as part of a joint effort with the Electric Power Research Institute to design a 300 MVA superconducting generator

  13. 1300MVA steam-turbine generators for Kansai Electric Power's Oi Nuclear Power Station

    International Nuclear Information System (INIS)

    Mitsubishi Electric has completed two 1300 MVA generators, equipped with 5500kW brushless exciters, that will be the No. 1 and No. 2 generators of the Oi plant. They are among the largest anywhere, and incorporate such technological innovations as water cooling of the stator coil and asymmetrical arrangement of the rotor slots. The article discusses generator specifications and construction, the brushless exciter, and the results of factory tests. (auth.)

  14. The foundations of a 95 MVA motor-generator set for pulsed duty

    CERN Document Server

    Probst, P H

    1972-01-01

    The vibration forces produced by the 95 MVA motor-generator set for the main ring magnets of the 28 GeV proton synchrotron at CERN in Geneva recur cyclically every few seconds and necessitate a special foundation for the set. Its function is to prevent any disturbing vibration energy reaching the accelerator and to eliminate any build- up in oscillation from successive vibration pulses. The final design incorporates a platform mounted on helical springs and fitted with additional dampers. (4 refs).

  15. Design and Development of a 100 MVA HTS Generator for Commercial Entry

    Energy Technology Data Exchange (ETDEWEB)

    None

    2007-06-07

    In 2002, General Electric and the US Department of Energy (DOE) entered into a cooperative agreement for the development of a commercialized 100 MVA generator using high temperature superconductors (HTS) in the field winding. The intent of the program was to: (1) identify and develop technologies that would be needed for such a generator; (2) develop conceptual designs for generators with ratings of 100 MVA and higher using HTS technology; (3) perform proof of concept tests at the 1.5 MW level for GE's proprietary warm iron rotor HTS generator concept; and (4) design, build, and test a prototype of a commercially viable 100 MVA generator that could be placed on the power grid. This report summarizes work performed during the program and is provided as one of the final program deliverables. The design for the HTS generator was based on GE's warm iron rotor concept in which a cold HTS coil is wound around a warm magnetic iron pole. This approach for rotating HTS electrical machinery provides the efficiency benefits of the HTS technology while addressing the two most important considerations for power generators in utility applications: cost and reliability. The warm iron rotor concept uses the least amount of expensive HTS wire compared to competing concepts and builds on the very high reliability of conventional iron core stators and armature windings.

  16. Internet Use Habits of Students of the Department of Information Management, Hacettepe University, Ankara

    Science.gov (United States)

    Ucak, Nazan Ozenc

    2007-01-01

    The frequency and other characteristics of Internet use of students studying at the Department of Information Management at Hacettep University in Ankara, Turkey, are examined. According to the findings, students prefer electronic media to printed media, they find the easy accessibility of the information more important than the other qualities,…

  17. A New Understanding of Ankara Music within the Context of Global Cultural Flow

    Directory of Open Access Journals (Sweden)

    Ömer Can Satır

    2015-12-01

    Full Text Available The aim of this study is to consider a new understanding of the complex and multilayered structure of Ankara music, which is situated between tradition and modernity and has become an important form of popular culture in the region, in the context of local and global relationships.. The theoretical framework of the study is based on Appadurai’s “global cultural flow” model. The ethnoscape, finanscape, mediascape, technoscape and ideoscape of Ankara music is examined in accordance with this approach to reveal the dynamics that make this music different. In the light of these findings, it is concluded that the ethnoscape of Ankara music is based on a broad demographic coalition that has created an independent music industry with a finanscape depending on production, distribution and consumption. While this type of music is represented through a network-based social structure, it gains legitimacy outside its bounds within the media environment. The “Ankara” genre and its quest for authenticity described in this work reveal the ideoscape of the new Ankara music.

  18. Service Quality of State Universities in Turkey: The Case of Ankara

    Science.gov (United States)

    Eres, Figen

    2011-01-01

    The aim of this study is determine the service quality perceptions of students being educated at state universities in Ankara. The sample of the study is composed of final grade students of faculties of Economics and Administrative Sciences. The sample of the study is composed of a total of randomly selected students. There were 416 returned…

  19. Estudio preliminar sobre micorriza versículo – Arbuscular (MVA en lulo (Solanum quitoense Lam

    Directory of Open Access Journals (Sweden)

    Sieverding Ewald

    1986-12-01

    Full Text Available En el estudio se determinó el carácter (obligado o facultativo de la asociación micorrícica y se evaluó la eficiencia de nueve cepas de micorriza en dos tipos de oxisoles con fertilidad baja y moderada y en presencia de tres niveles de fósforo (0,50 Y 100 kg/ha. El lulo es una especie micotrófica facultativa, ya que con adecuados niveles de fósforo asimilable en el suelo su desarrollo no depende de la asociación micorrícica, mientras que con bajos niveles sucede lo contrario. En general, la inoculación con MVA incrementó el desarrollo del lulo tanto en semilleros como en plántulas. Los mejores aislamientos de MVA fueron una mezcla de Acaulospora spp y Glomus sp y Entrophospora colombiana. En general, la efectividad de la MVA incrementó con niveles bajos y medios de fósforo y se deprimió con el nivel alto, El desarrollo del lulo y la efectividad de la micorriza fue mayor en la localidad más cálida (1050 m, 24°C y 60 % HR que en la más templada (2100 m, 14°C Y 80 % HR.A preliminary study to determine the character (obligate or facultative of the mycorrhizal association in, "naranjilla" (Solanum quitoense L. and an evaluation of nine mycorrhiza stock, was carried out at Palmira (1050 m, 24°C and R.H. of 60 % and Jamundí (2100 m, 14°C and R.H. of 80 %. The trial was held on two oxyzole having a lowand moderate fertility, with 3 levels of phosphorus (0,50 Y 100 kq/ha. The "naranjilla" is a facultative mycotrophic specie. In general, the inoculation with MVA fungi, increased the development of the "naranjilla" in seed beds as well as transplanted seed lings. The effectivity of MVA was dependent on the fungus specie, its origin, the phosphorus levels in the soil and the weather of both Iocalities. The development of the "naranjilla" and the effectivity of the mycorrhiza was superior at the locality (14°C. In general, the isolations which showed the best behavior were a mixture of Acaulospora spp and Glomus sp, and

  20. Modeling of gas demand using degree-day concept: case study for Ankara

    International Nuclear Information System (INIS)

    The demand for natural gas is rapidly increasing in Turkey, as it is in the rest of the world. However, natural gas reserves and production are rather limited in Turkey.The bulk of the Turkish gas demand is met by imports. Russia currently accounts for 69% of Turkey's gas supplies. Physical shortages might occur; supplies for industrial production and household consumption could temporarily run short. Also, fluctuations in consumption might occur due to climatic reasons or peak daily industrial energy demand. Underground gas storage is a necessity in order to regulate these seasonal, daily, and hourly fluctuations. In order to effectively design and utilize underground gas storage, it is necessary to identify the market requirements. In this study, Ankara was chosen as a pilot region due to its strategical importance of being the capital city of Turkey, and a wide range of marketing surveys for the last seven years was performed. All of the factors influencing the gas consumption and the relationships between these factors were analyzed. How does gas demand behave in extremely cold weather? How does the industrial part of the city act in the consumption behavior? What are the plans of the Municipality of Ankara, responsible for the execution of the natural gas distribution project in Ankara? A model was developed based on degree-day (DD) concept, including the annual number of customers, average DDs, and the usage per customer. A history matching study was performed to verify the results of the model with the measured consumption data for the last seven years. Comparisons showed that the calculated consumption by DD model and measured daily consumption were in good agreement. Finally, by using the developed approach, the gas demand was forecasted for Ankara up to 2005. The results of this study can be used to design underground gas storage facility near Ankara. (author)

  1. Método isotónico modificado para la Medida de actividades de electrolitos

    Directory of Open Access Journals (Sweden)

    Luis H. Blanco

    2009-06-01

    Full Text Available El método Isopléstico tradicional fue modificado en varios aspectos, siendo el más Importante el de reemplazar las celdas de metales preciosos por celdas de acero Inoxidable. Se adicionó un manómetro para poder detectar cambios bruscos de la presión, se cambió la agitación continua a esporádica y se estableció un control de la temperatura del aire. Los resultados obtenidos fueron comparables con los reportados en la literatura.

  2. Organismos modificados genéticamente: oportunidades ambientales y dificultades políticas

    OpenAIRE

    Rey García, Paula

    2002-01-01

    Los Organismos Modificados Genéticamente (OMG) en alimentos y otros productos son objeto en la actualidad de un candente debate. De acuerdo con la Directiva 2001/18/CE, el término OMG significa "un organismo, con la excepción de los seres humanos, en el cual el material genético ha sido alterado de un modo que no ocurre naturalmente a través de cruce y/o recombinación natural". Esta definición es también aceptada por la UNEP (Programa de las Naciones Unidas para el Medio Ambiente). ¿Qué hay d...

  3. Toxicidade de nanomateriais de ácidos poliacrílicos hidrofobicamente modificados

    OpenAIRE

    Tavares, Jorge Abel dos Santos Silva

    2014-01-01

    A crescente demanda por novos e melhores produtos levou a um desenvolvimento exponencial de novos nanomaterials (NM) manufacturados desde a década de 90. A corrente massificação de NM em produtos de uso quotidiano promove o contacto entre NM e o ambiente com possíveis efeitos adversos para os ecossistemas. O trabalho apresentado pretende sugerir qual o NM de ácido poliacrílico hidrofobicamente modificado (HMPAA) menos prejudicial para o ambiente de entre seis HMPAA NM, dos q...

  4. OS DIREITOS DO CONSUMIDOR E OS ORGANISMOS GENETICAMENTE MODIFICADOS – CONSUMER RIGHTS AND GENETICALLY MODIFIED ORGANISMS

    OpenAIRE

    Leonardo Estevam de Assis Zanini

    2012-01-01

    Com o avanço da ciência, desenvolveu-se a engenharia genética, a qual, não obstante os inúmeros benefícios que proporciona, também representa grandes riscos para os seres humanos. Dentre os avanços trazidos pela engenharia genética estão os organismos geneticamente modificados, muitas vezes tratados simplesmente como transgênicos. Questões de ordem econômica, social, ética, científica e ambie...

  5. Actitud del consumidor frente a los alimentos genéticamente modificados

    OpenAIRE

    Viedma, Inmaculada; López Nicolás, José Manuel; Serrano Megías, Marta; Balanza Galindo, Serafín

    2014-01-01

    Uno de los grandes retos en materia de seguridad alimentaria con los que se enfrenta la sociedad del siglo XXI es la aparición de una gran variedad de nuevos alimentos entre los que se encuentran los alimentos genéticamente modificados. Sin embargo, el futuro de estos alimentos va a depender de cómo sean percibidos por los consumidores y de la aceptación de los mismos. En este trabajo se analiza el grado de conocimiento que el consumidor de la Región de Murcia tiene sobre los alimentos genéti...

  6. Reflexión bioética sobre el uso de organismos genéticamente modificados

    Science.gov (United States)

    Yunta, Eduardo Rodríguez

    2011-01-01

    El presente artículo reflexiona desde los 4 principios de la bioética el uso comercial de organismos genéticamente modificados. Se cuestiona fundamentalmente la falta de transferencia de tecnología entre el mundo desarrollado y en desarrollo y el que el presente sistema de patentamiento de organismos vivos modificados fomenta intereses comerciales y no da debida importancia al desarrollo sostenible de la agricultura y ganadería en los países en desarrollo, donde más se necesita. Se reflexiona sobre la importancia que tiene evaluar los riesgos antes de introducirse en el mercado organismos genéticamente modificados y la necesidad de regulación en los países. PMID:21927675

  7. Immunogenic profiling in mice of a HIV/AIDS vaccine candidate (MVA-B expressing four HIV-1 antigens and potentiation by specific gene deletions.

    Directory of Open Access Journals (Sweden)

    Juan García-Arriaza

    Full Text Available BACKGROUND: The immune parameters of HIV/AIDS vaccine candidates that might be relevant in protection against HIV-1 infection are still undefined. The highly attenuated poxvirus strain MVA is one of the most promising vectors to be use as HIV-1 vaccine. We have previously described a recombinant MVA expressing HIV-1 Env, Gag, Pol and Nef antigens from clade B (referred as MVA-B, that induced HIV-1-specific immune responses in different animal models and gene signatures in human dendritic cells (DCs with immunoregulatory function. METHODOLOGY/PRINCIPAL FINDINGS: In an effort to characterize in more detail the immunogenic profile of MVA-B and to improve its immunogenicity we have generated a new vector lacking two genes (A41L and B16R, known to counteract host immune responses by blocking the action of CC-chemokines and of interleukin 1beta, respectively (referred as MVA-B DeltaA41L/DeltaB16R. A DNA prime/MVA boost immunization protocol was used to compare the adaptive and memory HIV-1 specific immune responses induced in mice by the parental MVA-B and by the double deletion mutant MVA-B DeltaA41L/DeltaB16R. Flow cytometry analysis revealed that both vectors triggered HIV-1-specific CD4(+ and CD8(+ T cells, with the CD8(+ T-cell compartment responsible for >91.9% of the total HIV-1 responses in both immunization groups. However, MVA-B DeltaA41L/DeltaB16R enhanced the magnitude and polyfunctionality of the HIV-1-specific CD4(+ and CD8(+ T-cell immune responses. HIV-1-specific CD4(+ T-cell responses were polyfunctional and preferentially Env-specific in both immunization groups. Significantly, while MVA-B induced preferentially Env-specific CD8(+ T-cell responses, MVA-B DeltaA41L/DeltaB16R induced more GPN-specific CD8(+ T-cell responses, with an enhanced polyfunctional pattern. Both vectors were capable of producing similar levels of antibodies against Env. CONCLUSIONS/SIGNIFICANCE: These findings revealed that MVA-B and MVA-B DeltaA41L/DeltaB16R

  8. Desempenho de misturas betuminosas com betume modificado com borracha através do processo húmido

    OpenAIRE

    Fontes, Liseane P. T. L.; Trichês, Glicério; Pereira, Paulo A. A.; Pais, Jorge C.

    2007-01-01

    Este trabalho apresenta os resultados da avaliação de desempenho mecânico de misturas betuminosas produzidas com betume modificado com borracha de pneus usados através do processo húmido, utilizando o sistema terminal blend e o sistema continuous blend. Os betumes modificados com borracha através dos dois sistemas foram caracterizados através dos seguintes ensaios: (i) penetração; (ii) ponto de amolecimento (método anel e bola); (iii) resiliência; (iv) viscosidade Brookfield. A avaliação do c...

  9. Simulation of double-fed speed regulation system for 125 MVA motor-generator set

    International Nuclear Information System (INIS)

    A simulation modeling of double-fed motor speed regulation for 125 MVA AC pulsed M-G set of HL-2A is built using Matlab. Some parameters of the project are obtained by the pre-simulation, and the process of start-up is analyzed. In the Matlab simulation the motor model in Simulink can not be connected directly to the cycloconverter model. This problem is solved by adding an ideal transformer model between them, and the satisfactory result is obtained. A new method of control switching two cycloconverter without circulating current has been introduced. (authors)

  10. Flywheel for a 167 MVA surge power motor-generator set

    International Nuclear Information System (INIS)

    Flywheels to be subjected to major speed fluctuations are designed on the basis of both the usual strength analysis and fracture mechanics considerations, and the testing and operating instructions have to allow for this. Appropriate test units are used to determine the fracture toughness of the material. Residual stresses are measured and extensive ultrasonic and magnetic particle tests performed to enable the permissible number of stress cycles to be predicted. The article deals with these problems by reference to the flywheel of a 167 MVA surge power motor-generator set for the Max Planck Insitute for Plasma Physics in Garching. (orig.)

  11. Excitation and voltage regulation for a 95 MVA surge-power generator

    CERN Document Server

    Bayer, K H

    1972-01-01

    The excitation equipment of the 95 MVA surge-power generator, which feeds the main ring magnets of the 28 GeV proton synchrotron at CERN in Geneva, is subject to certain abnormal requirements. They relate both to its dynamic control characteristics and to its accuracy of voltage regulation. These are essential in order to comply with the most stringent conditions made with respect to reproducibility of magnet cycles over the whole duration of an experimenting period of three to four weeks. A special control concept also ensures optimum results in this respect for the dynamic transition regions within a pulse cycle. (4 refs).

  12. [Studies of Yersinia pestis in wild animals captured in Ankara, Konya and Nevsehir].

    Science.gov (United States)

    Ozsan, K; Fazli, A; Aktan, M; Beyoğlu, K

    1976-01-01

    No Yersinia pestis could be isolated, by culturing and by inoculations to 1212 guinea-pigs and 150 mice; from 623 citellus, 41 Mus musculus, 55 Microtus, 442 Meriones, 70 Rattus rattus, 56 turtle, 89 hare, 1 hamster, 1 hedgehog, 1 sea snake, altogether 790 dead, 589 alive, i.e. 1379. wild animals captured in Ankara, Konya (Karapinar), Urfa (Akçakale) and in Nevşehir. In 141 sera taken from citellus captured alive, and in 174 sera taken from guinea-pigs inoculated with spleen, liver and kidney suspensions of wild animals, 1/20 - 1/80 agglutination titers (one of the sera from a guinea-pig inoculated with hare organ suspension) were obtained. These findings, probably were due to Yersinia pseudotuberculosis, because this organism was isolated from citellus captured in Ankara and Konya. PMID:933892

  13. Taxonomy, distribution, and ecology of crustacean zooplankton in trough waters of Ankara (Turkey)

    OpenAIRE

    BAŞAK, Elif; Aygen, Cem; KÜLKÖYLÜOĞLU, OKAN

    2014-01-01

    Troughs are one of the main components of villages in Turkey. They are constructed by converting springs or underground waters. Until now, there has been no extensive study investigating the composition and diversity of trough zooplankton species. In order to contribute knowledge on the zooplanktons in troughs, 142 troughs were randomly sampled from 17 districts in Ankara Province between 22 June and 3 July 2011. A total of 18 zooplanktons including 11 Copepoda and 7 Cladocera species were de...

  14. Establıshment Of Ankara University Faculty Of Health Sciences Students Breakfast Habits

    OpenAIRE

    FAYDAOĞLU, Emine; ENERGİN, Esma; SÜRÜCÜOĞLU, Metin Saip

    2013-01-01

    The purpose of this research was to determine the breakfast habits of the students being educated in the Faculty of Health Sciences, at Ankara University. The research population consisted of ten students from each undergraduate class of the Departments of Nutrition and Diatetics, Nursing, Midwifery, Social Service and Medical Institution Management and Pediatric Development of the Faculty of Health Sciences, making up 220 voluntary students in total. The individuals who participated in the r...

  15. Determination of some yield components of winter vetch species (Vicia spp.) grown in Ankara conditions

    OpenAIRE

    KENDİR, Hayrettin

    1999-01-01

    Vetch is one of the most widespread annual legume cultivated in our country. This research was conducted to determine seed yields and to observe important characteristics of 3 winter vetch species (Vicia spp.) in Ankara conditions from 1996-1998. Common vetch (Vicia sativa) , Hungarian vetch (Vicia pannonica) and hairy vetch (Vicia villosa), grown for seed and hay in Central Anatolia, were sown in autumn and grown using standard agricultural practices. The study incorporated a randomized plo...

  16. Determination of Microbiological Quality of Ice-Cream Belong to Various Patisserie in Ankara

    OpenAIRE

    Erol, İrfan; Küplülü, Özlem; SIRIKEN, Belgin; ÇELİK, T. Halûk

    1998-01-01

    In this study, a total of 100 ice cream samples, collected from various patisserie in Ankara, 30 of them vanilla, 26 of them chocolate and 44 of them fruit ice cream samples, were analysed microbiologically. While coliform and E. coli counts were determined with MPN technique, in the isolation of the salmonella enrichment technique and in the isolation of the total viable count, lactobacilli, micrococci and staphylococci, coagulase positive staphylococci, enterobacteriaceae, enterococci, p...

  17. Recognition of cigarette brand names and logos by primary schoolchildren in Ankara, Turkey

    OpenAIRE

    Emri, S; Bagci, T.; Karakoca, Y.; Baris, E

    1998-01-01

    OBJECTIVE—To assess the smoking behaviour of primary schoolchildren and their ability to recognise brand names and logos of widely advertised cigarettes, compared with other commercial products intended for children.
DESIGN—Cross-sectional survey in classroom settings using a questionnaire designed to measure attitudes towards smoking and the recognition of brand names and logos for 16 food, beverage, cigarette, and toothpaste products.
SETTING—Ankara, Turkey.
SUBJECTS—1093 children (54.6% bo...

  18. Operational Risk Management in Hotel Establishments: A Study on Four and Five Star Hotels in Ankara

    OpenAIRE

    Elbeyi Pelit

    2011-01-01

    The purpose of this study is to determine the applications of hotel establishments in the scope of operational risk. In this context, a survey was applied to the managers of four and five star hotel establishments in Ankara/Turkey. According to the factor analysis done after the literature search and questionnaire, four risks types (staff, technological, organizational and legal risks) have been found in the scope of operational risk, and their applications towards these risk types in hotel e...

  19. Effects of Tourism on Cultural Heritage: Awareness of Local People in Beypazari,Ankara

    OpenAIRE

    Uslu, A; Kiper, T.

    2006-01-01

    This study examines the impact of tourism on residents attiude in a small community in Turkey. Thefocus is on a rural town, known as Beypazari, currently emerging as a major tourist destination for its naturaland cultural landscape in the periphery of the capitol city of Ankara. Exponential increase (almost 1000% anincrease within the last five-years) in touristic activities in the city exaburated a citizen reaction. In thisarticle; the perceptions of citiezens about the effect of tourism hav...

  20. Giulio Mongeri, an Architect Efficient in the Building of Capital City Ankara and his Biography

    Directory of Open Access Journals (Sweden)

    Damla Çinici

    2015-06-01

    Full Text Available The study is about Giulio Mongeri (1873 – 1951 who was the architect of Ottoman Bank (1926, Ziraat Bank (1926 – 1929, Monopolies Chief Directorate (1928 and Türkiye İş Bank (1929 buildings in Ankara in the first years of the Republic. Giulio Mongeri was one of the architects prominent in architectural activities both in the last years of Ottoman Empire and first years of the Republic and undertook an effective role in the architectural understanding of the next generation by raising the architects of the future as an instructor of the Academy of Fine Arts. In the study, it is aimed to gain especially the sketches and photos obtained from his diary and not published anywhere else to the scientific world as a new contribution to the knowledge in the available publications. Giulio Mongeri himself, his family and environs in which he was raised, his personality and attitude as a trainer have been appraised in the light of new information. The buildings of Mongeri in Ankara have been studied comparatively with the other buildings in Ankara in this period and an attempt is made to assess the differences in his approach.

  1. Immunity against heterosubtypic influenza virus induced by adenovirus and MVA expressing nucleoprotein and matrix protein-1.

    Science.gov (United States)

    Lambe, Teresa; Carey, John B; Li, Yuanyuan; Spencer, Alexandra J; van Laarhoven, Arjan; Mullarkey, Caitlin E; Vrdoljak, Anto; Moore, Anne C; Gilbert, Sarah C

    2013-01-01

    Alternate prime/boost vaccination regimens employing recombinant replication-deficient adenovirus or MVA, expressing Influenza A virus nucleoprotein and matrix protein 1, induced antigen-specific T cell responses in intradermally (ID) vaccinated mice; with the strongest responses resulting from Ad/MVA immunization. In BALB/C mice the immunodominant response was shifted from the previously identified immunodominant epitope to a novel epitope when the antigen was derived from A/Panama/2007/1999 rather than A/PR/8. Alternate immunization routes did not affect the magnitude of antigen-specific systemic IFN-γ response, but higher CD8(+) T-cell IFN-γ immune responses were seen in the bronchoalveolar lavage following intransal (IN) boosting after intramuscular (IM) priming, whilst higher splenic antigen-specific CD8(+) T cell IFN-γ was seen following IM boosting. Partial protection against heterologous influenza virus challenge was achieved following either IM/IM or IM/IN but not ID/ID immunization. These data may be of relevance for the design of optimal immunization regimens for human influenza vaccines, especially for influenza-naïve infants. PMID:23485942

  2. Double-Difference Tomography for Sequestration MVA [monitoring, verification, and accounting

    Energy Technology Data Exchange (ETDEWEB)

    Westman, Erik

    2012-12-31

    Analysis of synthetic data was performed to determine the most cost-effective tomographic monitoring system for a geologic carbon sequestration injection site. Double-difference tomographic inversion was performed on 125 synthetic data sets: five stages of CO2 plume growth, five seismic event regions, and five geophone arrays. Each resulting velocity model was compared quantitatively to its respective synthetic velocity model to determine an accuracy value. The results were examined to determine a relationship between cost and accuracy in monitoring, verification, and accounting applications using double-difference tomography. The geophone arrays with widely-varying geophone locations, both laterally and vertically, performed best. Additionally, double difference seismic tomography was performed using travel time data from a carbon sequestration site at the Aneth oil field in southeast Utah as part of a Department of Energy initiative on monitoring, verification, and accounting (MVA) of sequestered CO2. A total of 1,211 seismic events were recorded from a borehole array consisting of 22 geophones. Artificial velocity models were created to determine the ease with which different CO2 plume locations and sizes can be detected. Most likely because of the poor geophone arrangement, a low velocity zone in the Desert Creek reservoir can only be detected when regions of test site containing the highest ray path coverage are considered. MVA accuracy and precision may be improved through the use of a receiver array that provides more comprehensive ray path coverage.

  3. Safety and tolerability of conserved region vaccines vectored by plasmid DNA, simian adenovirus and modified vaccinia virus ankara administered to human immunodeficiency virus type 1-uninfected adults in a randomized, single-blind phase I trial.

    Directory of Open Access Journals (Sweden)

    Emma-Jo Hayton

    Full Text Available TRIAL DESIGN: HIV-1 vaccine development has advanced slowly due to viral antigenic diversity, poor immunogenicity and recently, safety concerns associated with human adenovirus serotype-5 vectors. To tackle HIV-1 variation, we designed a unique T-cell immunogen HIVconsv from functionally conserved regions of the HIV-1 proteome, which were presented to the immune system using a heterologous prime-boost combination of plasmid DNA, a non-replicating simian (chimpanzee adenovirus ChAdV-63 and a non-replicating poxvirus, modified vaccinia virus Ankara. A block-randomized, single-blind, placebo-controlled phase I trial HIV-CORE 002 administered for the first time candidate HIV-1- vaccines or placebo to 32 healthy HIV-1/2-uninfected adults in Oxford, UK and elicited high frequencies of HIV-1-specific T cells capable of inhibiting HIV-1 replication in vitro. Here, detail safety and tolerability of these vaccines are reported. METHODS: Local and systemic reactogenicity data were collected using structured interviews and study-specific diary cards. Data on all other adverse events were collected using open questions. Serum neutralizing antibody titres to ChAdV-63 were determined before and after vaccination. RESULTS: Two volunteers withdrew for vaccine-unrelated reasons. No vaccine-related serious adverse events or reactions occurred during 190 person-months of follow-up. Local and systemic events after vaccination occurred in 27/32 individuals and most were mild (severity grade 1 and predominantly transient (<48 hours. Myalgia and flu-like symptoms were more strongly associated with MVA than ChAdV63 or DNA vectors and more common in vaccine recipients than in placebo. There were no intercurrent HIV-1 infections during follow-up. 2/24 volunteers had low ChAdV-63-neutralizing titres at baseline and 7 increased their titres to over 200 with a median (range of 633 (231-1533 post-vaccination, which is of no safety concern. CONCLUSIONS: These data demonstrate

  4. Diseño y desarrollo de un análogo de queso para pizza a partir de almidones modificados

    OpenAIRE

    Rodríguez Iglesias, Paula

    2013-01-01

    El objetivo principal de este trabajo es el diseño y desarrollo de una fórmula de fabricación de un análogo de queso a partir de almidones modificados que consiga reducir los costes conservando unas propiedades funcionales y fisicoquímicas aptas para su comercialización. Se centra en la sustitución de parte de la caseína cuajo por una combinación de almidones modificados realizándose una serie de pruebas previas con distintos almidones modificados y se seleccionan los que mejor funcionan: ...

  5. Fluorescent lighting system of modified fast starting system; Sistema de alumbrado fluorescente de arranque rapido modificado

    Energy Technology Data Exchange (ETDEWEB)

    Mendoza E, Ernesto J. [Manufacturera De Reactores, S. A., Mexico, D. F. (Mexico)

    1993-12-31

    This paper puts into perspective the possible energy saving in using hybrid ballasts for the operation of fast starting lamps. It presents in summarized form some fundamentals of the fluorescent lamps operation, as well as the fast starting modified systems. Also the results of field operation are shown, as well as the effects on the useful life of the lamp T12 as well as the T8. [Espanol] Este trabajo pone en perspectiva el ahorro de energia posible, al emplear balastros hibridos para operar lamparas de arranque rapido. Presenta en forma resumida algunos fundamentos de la operacion de lamparas fluorescentes, asi como de los sistemas de arranque rapido modificado. Se muestran tambien resultados de operacion de campo, asi como los efectos sobre la vida util de las lamparas, tanto T12 como T8.

  6. OS DIREITOS DO CONSUMIDOR E OS ORGANISMOS GENETICAMENTE MODIFICADOS – CONSUMER RIGHTS AND GENETICALLY MODIFIED ORGANISMS

    Directory of Open Access Journals (Sweden)

    Leonardo Estevam de Assis Zanini

    2012-08-01

    Full Text Available Com o avanço da ciência, desenvolveu-se a engenharia genética, a qual, não obstante os inúmeros benefícios que proporciona, também representa grandes riscos para os seres humanos. Dentre os avanços trazidos pela engenharia genética estão os organismos geneticamente modificados, muitas vezes tratados simplesmente como transgênicos. Questões de ordem econômica, social, ética, científica e ambiental criaram um grande debate em torno desses organismos, levando à formação de dois blocos de países, um favorável e outro contrário aos organismos geneticamente modificados (OGMs. Juridicamente, também foram concebidos princípios para a sustentação do posicionamento dos referidos países. No que diz respeito aos direitos do consumidor, a maior polêmica na seara dos transgênicos é a obrigatoriedade de rastreamento e rotulação, o que garantiria ao consumidor o direito de escolher consumir ou não produtos transgênicos. Destarte, diante das possibilidades e riscos trazidos pelos OGMs, vamos, no presente trabalho, tratar especificamente das relações existentes entre os direitos de informação e escolha do consumidor e o fornecimento de produtos geneticamente modificados.PALAVRAS-CHAVE: Direito do consumidor. Direito à informação. Organismos geneticamente modificados. Transgênicos. Princípio da precaução. Princípio da equivalência substancial. Rotulação.ABSTRACT: The science developed the genetic engineering, which provides numerous benefits, but also poses great risks to humans. Among the advances brought by the genetic engineering are the genetically modified organisms, which are often treated simply as transgenics. A great debate around these organisms was created by economic, social, ethical, scientific and environmental questions, which led to the formation of two blocs of countries, one favorable and one contrary to the genetically modified organisms. Legal principles are also designed to support the position

  7. First operation experiences from a 30 kV 104 MVA HTS power cable installed in a utility substation

    DEFF Research Database (Denmark)

    Willen, D.; Hansen, F.; Daumling, M.; Rasmussen, C.N.; Østergaard, Jacob; Træholt, Chresten; Veje, E.; Tønnesen, Ole; Jensen, K.H.; Olsen, S.K.; Rasmussen, C.; Hansen, E.; Schuppach, O.; Visler, T.; Kvorning, S.; Schuzster, J.; Mortensen, J.; Christiansen, J.; Mikkelsen, S.D.

    An HTS cable with a voltage rating of 30 kV and a power rating of 104 MVA, has been installed and operated in the electric grid of Copenhagen Energy in the spring of 2001. This article describes the development phases, the system specifications, and the first experiences of operation under...

  8. Aspects regarding the Calculation of the Dielectric Loss Angle Tangent between the Windings of a Rated 40 MVA Transformer

    Directory of Open Access Journals (Sweden)

    Cristinel Popescu

    2015-09-01

    Full Text Available The paper aims to identify how to determine the dielectric loss angle tangent of the electric transformers from the transformer stations. Autors of the paper managed a case study on the dielectric established between high respectively medium voltage windings of an electrical rated 40 MVA transformer.

  9. Biochemical and Structural Basis for Inhibition of Enterococcus faecalis Hydroxymethylglutaryl-CoA Synthase, mvaS, by Hymeglusin

    Energy Technology Data Exchange (ETDEWEB)

    Skaff, D. Andrew; Ramyar, Kasra X.; McWhorter, William J.; Barta, Michael L.; Geisbrecht, Brian V.; Miziorko, Henry M. (UMKC)

    2012-07-25

    Hymeglusin (1233A, F244, L-659-699) is established as a specific {beta}-lactone inhibitor of eukaryotic hydroxymethylglutaryl-CoA synthase (HMGCS). Inhibition results from formation of a thioester adduct to the active site cysteine. In contrast, the effects of hymeglusin on bacterial HMG-CoA synthase, mvaS, have been minimally characterized. Hymeglusin blocks growth of Enterococcus faecalis. After removal of the inhibitor from culture media, a growth curve inflection point at 3.1 h is observed (vs 0.7 h for the uninhibited control). Upon hymeglusin inactivation of purified E. faecalis mvaS, the thioester adduct is more stable than that measured for human HMGCS. Hydroxylamine cleaves the thioester adduct; substantial enzyme activity is restored at a rate that is 8-fold faster for human HMGCS than for mvaS. Structural results explain these differences in enzyme-inhibitor thioester adduct stability and solvent accessibility. The E. faecalis mvaS-hymeglusin cocrystal structure (1.95 {angstrom}) reveals virtually complete occlusion of the bound inhibitor in a narrow tunnel that is largely sequestered from bulk solvent. In contrast, eukaryotic (Brassica juncea) HMGCS binds hymeglusin in a more solvent-exposed cavity.

  10. Comportamiento de mezclas asfálticas fabricadas con asfaltos modificados con ceras

    Directory of Open Access Journals (Sweden)

    Oscar Javier Reyes Ortíz

    2013-01-01

    Full Text Available El propósito principal de la investigación fue modificar asfaltos colombianos con ceras naturales para fabricar mezclas asfálticas tibias y establecer su comportamiento mecánico y dinámico. Las mezclas estudiadas corresponden a las denominadas curvas granulometrías md-10 y md-12 del Instituto de Desarrollo Urbano (IDU. El estudio inicio con la caracterización de los materiales a utilizar (granular, asfalto y ceras naturales, seguido de la determinación del porcentaje de adición de ceras y el contenido de asfalto óptimo para fabricar mezclas tibias. Posteriormente se fabricaron y compactaron las mezclas asfálticas a 110, 130 y 150°C para evaluar el efecto del asfalto modificado en su comportamiento mecánico y dinámico. Los ensayos utilizados para medir las propiedades de las mezclas asfálticas fueron la resistencia a la tracción indirecta, la resisten- cia conservada y los módulos resilientes. Del análisis de los resultados se estableció que las ceras naturales utilizadas reducen la viscosidad de los asfaltos y por ende las temperaturas de fabricación y compactación de las mezclas, reduciendo significativamente el consumo de energía y la generación de gases de efecto invernadero. De otra parte, se estableció que las propiedades mecánicas y dinámicas estudiadas a las mezclas asfálticas con asfalto modificado con ceras, son similares a las mezclas convencionales, estableciendo la viabilidad para su aplicación y uso.

  11. The modification on two 80 MVA motor-generator sets in HL-2A

    International Nuclear Information System (INIS)

    Two 80 MVA motor-generator sets have been modified successfully in order to satisfy the pulse power supply demand for toroidal system under the high parameter discharge in HL-2A tokamak device. The 42 ton old flywheel is replaced by 88 ton new one, at the same time the double fed vector control technology based on a. c./a. c. cycloconverter is adopted on two 2500 KW drive motors. As a result, the maximum operating speed of each motor-generator set is raised from 1480 r/min to 1650 r/min, thus the 500 MJ released energy of each modified motor-generator set can be obtained during a pulse discharge and the toroidal field system has the capacity to provide 2.8 tesla in HL-2A. (authors)

  12. Investigation of slot discharge on a 239 MVA hydro generator stator winding

    Energy Technology Data Exchange (ETDEWEB)

    Li, S.; Hong, W. [BC Hydro and Power Authority, Vancouver, BC (Canada)

    2009-07-01

    This paper discussed a slot discharge investigation conducted on a 239 MVA generator stator winding. The generator in which the winding was located had experienced core split distortion, stator winding phase-to-phase failures, winding failures during Hipot testing, and high partial discharge (PD) activity. The results of on-line PD testing data were evaluated. The stator winding was subjected to visual inspections, bar dissections, and failure mechanism analyses. Eleven winding bars were removed from the stator slots in order to assess groundwall insulation conditions and identify the cause of the slot discharge activity. It was determined that the root cause of the slot discharge was a loose, non-uniform bar in the slot. The vibrating bar caused the semi-conductive coating to wear out and degraded the armour tape. Results of the study demonstrated the importance of on-line PD monitoring for detecting slot PD activity. 4 refs., 3 tabs., 11 figs.

  13. Dendritic cells exposed to MVA-based HIV-1 vaccine induce highly functional HIV-1-specific CD8(+ T cell responses in HIV-1-infected individuals.

    Directory of Open Access Journals (Sweden)

    Núria Climent

    Full Text Available Currently, MVA virus vectors carrying HIV-1 genes are being developed as HIV-1/AIDS prophylactic/therapeutic vaccines. Nevertheless, little is known about the impact of these vectors on human dendritic cells (DC and their capacity to present HIV-1 antigens to human HIV-specific T cells. This study aimed to characterize the interaction of MVA and MVA expressing the HIV-1 genes Env-Gag-Pol-Nef of clade B (referred to as MVA-B in human monocyte-derived dendritic cells (MDDC and the subsequent processes of HIV-1 antigen presentation and activation of memory HIV-1-specific T lymphocytes. For these purposes, we performed ex vivo assays with MDDC and autologous lymphocytes from asymptomatic HIV-infected patients. Infection of MDDC with MVA-B or MVA, at the optimal dose of 0.3 PFU/MDDC, induced by itself a moderate degree of maturation of MDDC, involving secretion of cytokines and chemokines (IL1-ra, IL-7, TNF-α, IL-6, IL-12, IL-15, IL-8, MCP-1, MIP-1α, MIP-1β, RANTES, IP-10, MIG, and IFN-α. MDDC infected with MVA or MVA-B and following a period of 48 h or 72 h of maturation were able to migrate toward CCL19 or CCL21 chemokine gradients. MVA-B infection induced apoptosis of the infected cells and the resulting apoptotic bodies were engulfed by the uninfected MDDC, which cross-presented HIV-1 antigens to autologous CD8(+ T lymphocytes. MVA-B-infected MDDC co-cultured with autologous T lymphocytes induced a highly functional HIV-specific CD8(+ T cell response including proliferation, secretion of IFN-γ, IL-2, TNF-α, MIP-1β, MIP-1α, RANTES and IL-6, and strong cytotoxic activity against autologous HIV-1-infected CD4(+ T lymphocytes. These results evidence the adjuvant role of the vector itself (MVA and support the clinical development of prophylactic and therapeutic anti-HIV vaccines based on MVA-B.

  14. An Assessment for Soft Drink Consumption of Individuals in Centre Town of Ankara Province

    OpenAIRE

    Bilge Gözener; Murat Sayılı

    2015-01-01

    In this study, the Central district of Ankara (Çankaya, Etimesgut, Gölbaşı, Keçiören, Mamak, Polatlı, Sincan and Yenimahalle districts) carbonated drink consumption situation of consumers were examined. The data obtained from questionnaires conducted with 272 families were used in this study. Data were collected in April-May 2012.Between carbonated drink consumption and some demographic characteristics of consumers were tested by chi-square analysis of whether a statistical association. Accor...

  15. Effect of Irrigation Water on Corn Grain Yield in Ankara Conditions

    OpenAIRE

    YILDIRIM, Y. Ersoy; KODAL, Süleyman

    1998-01-01

    This research was conducted in Ankara conditions in order to determine corn yield response to water. When 50% of available water content in root zone is consumed in the control treatment, irrigation water was applied to the other treatments with 0%, 25%, 50%, 75%, 125%, 150%, 175% and 200% of those applied into check plots. As a result, applications of ex-cessive water was not increased grain yields at the important level. Yield response factor (k y ) for total growing period were found a...

  16. A Survey of Bone Tumors in Dogs and Cats from 1986 to 2000 in Ankara

    OpenAIRE

    Kutsal, Osman

    2003-01-01

    In the present study, bone tumor samples obtained from 18 dogs and 21 cats from various clinics between 1986 and 2000 were clinically and pathologically examined at the Pathology Department, Faculty of Veterinary Medicine, University of Ankara. Most bone tumors examined were from 5-8-year-old dogs (43.75%) and cats (45%). A significant decrease was detected in animals over 8 years old. These tumors were observed in large breeds, mostly in German shepherd (38.4%) and Anatolian shepherd (Kanga...

  17. Prevalence and Antibiotic Resistance of Listeria Spp. Isolated from Ready-to-Eat Foods in Ankara

    OpenAIRE

    ŞİRELİ, Ufuk Tansel; GÜCÜKOĞLU, Ali

    2008-01-01

    In this study the presence of Listeria spp. is tested in 100 ready-to-eat food samples purchased from different stores and traditional food shops in the province of Ankara. The tested materials were 20 each of the following: mayonnaise based salad, kadınbudu köfte (fried meatball), fried liver, rice stuffed mussel, and green salad. Microbiological analyzes showed that 13 of 100 salad samples (13%) were contaminated with Listeria spp. while 10 of 100 salad samples (10%) were contaminated with ...

  18. Consumers’ Determination of Red Meat and Meat Products Purchase Behaviour – City of Ankara Sample

    OpenAIRE

    Hasan Arısoy; Zeki Bayramoğlu

    2014-01-01

    Nowadays, meat consuming level is an indicator of development of countries. The reason for this is the importance of animal proteins such as meat, milk and egg in human nutrition. People in Turkey do not get enough animal protein. Increase of meat and meat related product prices is effective on this. The purpose of this study is to bring up the purchase and consume behavior of consumers. Surveys completed in urban areas around city of Ankara are used primarily. Using Main Mass Ratio Based Si...

  19. Effects of Tourism on Cultural Heritage: Awareness of Local People in Beypazari,Ankara

    Directory of Open Access Journals (Sweden)

    A. Uslu

    2006-09-01

    Full Text Available This study examines the impact of tourism on residents attiude in a small community in Turkey. Thefocus is on a rural town, known as Beypazari, currently emerging as a major tourist destination for its naturaland cultural landscape in the periphery of the capitol city of Ankara. Exponential increase (almost 1000% anincrease within the last five-years in touristic activities in the city exaburated a citizen reaction. In thisarticle; the perceptions of citiezens about the effect of tourism have been examinated with random samplesurvey of residents.

  20. Los usuarios ante los alimentos genéticamente modificados y su información en el etiquetado

    Directory of Open Access Journals (Sweden)

    Miren Itxaso Sebastian-Ponce

    2014-02-01

    Full Text Available OBJETIVO : Analizar la opinión que los usuarios tienen sobre alimentos genéticamente modificados y su información en el etiquetado. MÉTODOS : Realizada revisión sistemática de la literatura científica sobre los alimentos transgénicos y el etiquetado a partir de la consulta de las bases de datos bibliográficas: Medline (vía PubMed, EMBASE, ISI-Web of Knowledge, Cochrane Library Plus, FSTA, LILACS, CINAHL y AGRICOLA. Los descriptores seleccionados fueron: «organisms, genetically modified » y «food labeling». La búsqueda se realizó desde la primera fecha disponible hasta junio de 2012, seleccionando los artículos pertinentes escritos en inglés, portugués y castellano. RESULTADOS : Se seleccionaron 40 artículos. En todos ellos, se debía haber realizado una intervención poblacional enfocada al conocimiento de los consumidores sobre los alimentos genéticamente modificados y su necesidad, o no, de incluir información en el etiquetado. El consumidor expresa su preferencia por el producto no-genéticamente modificado, y apunta que está dispuesto a pagar algo más por él, pero, en definitiva compra el artículo que está a mejor precio en un mercado que acoge las nuevas tecnologías. En 18 artículos la población se mostraba favorable a su etiquetado obligatorio y seis al etiquetado voluntario; siete trabajos demostraban el poco conocimiento de la población sobre los transgénicos y, en tres, la población subestimó la cantidad que consumía. En todo caso, se observó la influencia del precio del producto genéticamente modificado. CONCLUSIONES : La etiqueta debe ser homogénea y aclarar el grado de tolerancia en humanos de alimentos genéticamente modificados en comparación con los no modificados. Asimismo, debe dejar claro su composición, o no, de alimento genéticamente modificado y la forma de producción de estos artículos de consumo. La etiqueta también debe ir acompañada de un sello de certificación de una agencia

  1. Comportamiento de tejas de cubierta tipo multicapa fabricadas con asfalto crudo y modificado cuando son sometidas a cargas constantes

    OpenAIRE

    L??pez Bula, Pedro Javier

    2011-01-01

    El presente trabajo, analiza las propiedades viscoelasticas de diferentes materiales asfalticos despu??s de ser sometidos a cargas permanentes y a diferente temperatura -- Estas cargas simulan las que eventualmente se pueden presentar en la aplicacion y fabricaci??n de productos asfalticos ampliamente utilizados en el sector de la construcci??n -- Para este estudio se utiliz?? asfalto crudo y modificado con diferentes pol??meros: Cera de polietileno (CERA), caucho molido de llanta (CR), copol...

  2. Trihalomethanes and associated potential cancer risks in the water supply in Ankara, Turkey

    International Nuclear Information System (INIS)

    The occurrence of trihalomethanes (THMs) in the water supply in the Ankara, Turkey was investigated. Total THMs and total organic carbon measurements were carried seasonally in the samples collected form 22 different districts along with the samples taken from the Ivedik Water Treatment Plant serving 90% of the city. The average summer nonpurgeable organic carbon (NPOC) concentration in the raw water was 4.2 mg/L, and the NPOC removal achieved in the treatment plant was 31%. The concentration of total THMs ranged from 25 to 74 μg/L, from 28 to 73 μg/L, and from 25 to 110 μg/L in winter, spring, and summer, respectively. In all of the samples chloroform existed at the highest concentrations, while bromoform was almost absent. The total THM concentrations were highest in summer for all districts. However, none of the concentrations detected exceeded the USEPA's Stage I limit of 80 μg/L and the EU's limit of 100 μg/L. However, the total THM level in 64% of the districts exceeded the USEPA's Stage II limit of 40 μg/L. The risk estimations carried out indicated that each year 1 of the 5 million Ankara residents could get cancer from the daily intake of water, mainly because of exposure to chloroform through oral ingestion

  3. Nitrogen utilization of vegetables grown under plastic greenhouse conditions in Ankara using 15N technique

    International Nuclear Information System (INIS)

    In order to find suitable varieties of tomato, pepper and cucumber for plastic greenhouse conditions in Ankara and eventually to identify the best N fertilizer rate greenhouse experiments were conducted for two years. Yazgi F1 variety for tomato, Hizir F1 variety for cucumber and Serademre 8 variety for pepper were chosen to be the suitable varieties to grow in the plastic greenhouse conditions in Ankara. Five N treatments [N0=0, N1=150, N2=300, and N3=450 kg/ha; also, soil N application treatment (Nsoil) equivalent to the fertigation treatment of 300 kg/ha was included for tomato and pepper, however N rates for cucumber was 131, 266 and 339 kg N/ha; Nsoil being 266 kg N/ha] were investigated using 15N labeled urea fertilizer. Significantly higher marketable fresh fruit and total dry matter yields and N uptakes values were obtained from N3 treatments for tomato and cucumber, but from N2 treatment for pepper. Also, significantly higher yields, N uptakes and % NUE values were obtained when the same amount of N fertilizer is applied through fertigation compared to the treatment where N fertilizer applied to the soil then drip irrigated. (author)

  4. The analysis of impact of urbanization on the bioclimatic conditions in the scale of Ankara

    Directory of Open Access Journals (Sweden)

    Necla Türkoğlu

    2012-05-01

    Full Text Available Normal 0 21 false false false TR X-NONE X-NONE In this study the biclimatological conditions of Ankara urban area have been analyzed from the data which has been acquired from areas having different land cover and use. The hourly thermal perceptions of six meteorology stations have been calculated by using the physiologically equivalent temperature (PET index that could involve not only the meteorological parameters but also human energy balance in calculations. The spatial distribution of values has been carried out by the multiple linear regression models. At the end of the analyses it is concluded that the areas which have higher built density in urban have greater PET values than the other land use classes during the most of the day. These daily mean PET difference between these areas and the grasslands is 0.4°C -1.2°C, the parks is 2.2°C-3°C, suburban is 1.4°C -2.2°C, rural is 1.7°C -2.5°C. The differences increase during night and from May to September, and decrease during daytime and from October to April. Keywords: Thermal perception, bioclimatic conditions, land use, physiologically equivalent temperature, Ankara.

  5. New On-Line Excitation-System Ground Fault Location Method Tested in a 106 MVA Synchronous Generator

    OpenAIRE

    Blánquez Delgado, Francisco; Pardo, Miguel; Platero Gaona, Carlos Antonio; Rebollo López, Emilio; Blázquez García, Francisco

    2014-01-01

    In this paper, a novel excitation-system ground-fault location method is described and tested in a 106 MVA synchronous machine. In this unit, numerous rotor ground-fault trips took place always about an hour after the synchronization to the network. However, when the field winding insulation was checked after the trips, there was no failure. The data indicated that the faults in the rotor were caused by centrifugal forces and temperature. Unexpectedly, by applying this new method, the failure...

  6. Performance of the ±80MVA GCT STATCOM Under Commercial Operation

    Science.gov (United States)

    Fujii, Toshiyuki; Chisyaki, Hirokazu; Teramoto, Hitoshi; Sato, Tadashi; Matsushita, Yoshinao; Shinki, Yoriko; Funahashi, Sadao; Morishima, Naoki

    This paper presents a newly developed an 80MVA GCT STATCOM for the Kanzaki substation of the Kansai Electric Power Co., Inc. The commercial operation was started in June, 2004. The main purpose of the STATCOM is to maintain voltage during single circuit connection of source to the substation resulting from an AC system fault in order not to break down load system of the substation. The developed STATCOM has three distinctive features: (1) a high-efficient, compact and AC filter-less system using multistage three-level GCT converter and customized harmonics reduction PWM; (2) a coordinated voltage regulator with tap control of main transformers; and (3) a continuous operation from a single phase-ground fault through reclosing of the faulted circuit by limiter adjustments of the reactive current reference. This paper describes characteristics of the coordinated voltage control in the substation, and shows performance of the continuous operation during AC system faults using data of commercial operations and the simulation results.

  7. An Assessment for Soft Drink Consumption of Individuals in Centre Town of Ankara Province

    Directory of Open Access Journals (Sweden)

    Bilge Gözener

    2015-03-01

    Full Text Available In this study, the Central district of Ankara (Çankaya, Etimesgut, Gölbaşı, Keçiören, Mamak, Polatlı, Sincan and Yenimahalle districts carbonated drink consumption situation of consumers were examined. The data obtained from questionnaires conducted with 272 families were used in this study. Data were collected in April-May 2012.Between carbonated drink consumption and some demographic characteristics of consumers were tested by chi-square analysis of whether a statistical association. According to the survey; It was determined that 71.69% of the families consume carbonated drink. The vast majority of consumers (94.87%, carbonated drinks they consume more during the summer and is harmful to health (81.03% had continued to consume despite expressing. In addition, consumption of carbonated drinks with gender, age was found to be a statistically significant relationship between marital status and spouse's employment status.

  8. The frequency and effecting factors of consanguineous marriage in a group of soldiers in Ankara.

    Science.gov (United States)

    Kir, Tayfun; Güleç, Mahir; Bakir, Bilal; Hoşjgönül, Esat; Tümerdem, Nazmi

    2005-07-01

    This cross-sectional study was carried out to investigate the frequency of consanguineous marriage in a group of army conscripts in Ankara and the factors affecting this. Of 4153 soldiers, 387 were married. The rate of marriage between first cousins was found to be 19.1%, and the overall rate of consanguineous marriage was 24.1%. Consanguineous marriage was found to be significantly prevalent among soldiers who were born in and still living in the Eastern region; among those who lived in villages; among those whose parents as well as themselves had low educational levels; and among those whose marriages were arranged by their families. Neither the payment of bride-price nor the presence of consanguinity between parents was a significant factor for consanguineous marriage. In addition, the age of the soldier and the age at marriage were significantly lower among soldiers married to first cousins than among soldiers whose marriages were not consanguineous. PMID:16082860

  9. HOLIDAY HABITS OF CIVIL SERVANTS: A STUDY ON TEDAS EMPLOYEES IN ANKARA

    Directory of Open Access Journals (Sweden)

    Ayhan KARAKAŞ

    2012-06-01

    Full Text Available The purpose of this study is to detect holiday habits of officers, their motivation of going to holiday, factors effecting them to decide The research was applied on 119 civil servants working in general headquarters of TEDAŞ in Ankara by using questionnaire technique. Frequency table, Mann-Whitney U and chi-square test were used for the analysis of data. As a result of the research, it is detected that half of them spend their holidays by participating in tourism facilities; spend their free times by reading, doing sports, doing grizzle walks and having picnic; spend their holidays in resorts and hometowns; generally have holidays once a year; give the decision of holiday together with family and make their reservations directly or via travel agencies.

  10. The first Koç Han: Pioneering modern architecture in Ankara

    Directory of Open Access Journals (Sweden)

    Oya Atalay Franck

    2013-01-01

    Full Text Available Koç Holding is the most established conglomerate in Turkey. Th e company’s beginnings date from the mid 1920s, when Ahmet Vehbi Koç, founder of Koç Holding, had his first own company registered with the Ankara Chamber of Commerce. In 1932, Koç moved his business from his father’s store on Anafartalar Street to a new building, the first Koç Han, erected the same year on Çankırı Street Nr.13 in Ulus. Th e building’s designer was Swiss-Austrian architect Ernst Arnold Egli, who had come to Turkey only five years earlier upon invitation by the government to work as chief architect of the Ministry of National Education. During his stay, which lasted from 1927 until 1940, Egli realized about 40 projects and worked on many more. Koç Han is the rare example of a commercial building by Egli. At a time when the urban aspect of the old town center of Ankara consisted mostly of one-or two-storeyed stone-and-wood houses, the first Koç Han represented an altogether new building type for the town, in being a multipurpose edifice with space for shops at street level, with large storage facilities below ground, and providing off ices and housing on the upper fl oors. Th e functional rigor of the plan and the sober modernism of the building’s facades contrasted strongly with the architecture of the time. In this respect, the first Koç Han was a strong statement regarding Ahmet Vehbi Koç’s belief in a modern Turkey and to the contribution of the building’s architect, Ernst Arnold Egli, to this project.

  11. Association of Some Environmental Factors with Breath Carbon Monoxide Levels of Some Taxi Drivers in Ankara

    Directory of Open Access Journals (Sweden)

    Oguz Baran

    2010-12-01

    Full Text Available AIM: Taxi drivers are among the occupational groups with the highest smoking prevalence and exposure to carbon monoxide (CO. This study aimed to measure breath CO levels of some taxi drivers working in Ankara and to find out some associated factors (if any. METHOD: The descriptive study was carried out with 173 taxi drivers from 14 different taxi stations in the center of Ankara. Data was collected by face to face interviews with a standart questionnaire, while breath CO was measured by a Pi-CO Smokerlyser. Descriptive statistics were used to summarize data, whereas chi-square, independant samples t-test and One-Way ANOVA were used to compare groups by SPSS 15.0 statistical package programme. RESULTS: In the study, all of the taxi drivers (n=173 were male with a mean age of 39.2±9.6 years. Of the drivers, 58.4% were current smokers, whereas 75.1% were exposed to enviromental tobacco smoke. The frequency of indoor smoking in the taxi stations, taxis and drivers’ homes were 48.0%, 45.1%, and 59.0%, respectively. The mean breath CO level of the drivers was 16.9±12.8 ppm. CO level was positively associated with the current smoking status, total years of smoking, number of cigarettes smoked per day and passive exposure to tobacco smoke, whereas the association was negative with the elapsed time from the last cigarette smoked (p0.05. CONCLUSION: Results of the study provide evidence in support of the previous literature that smoking is one of the most important sources of carbonmonoxide. Interventions such as awareness raising trainings, referral of smokers willing to quit smoking to smoking cessation centers and screening programmes for smoking related diseases are needed to be implemented in collaboration with the relevant drivers’ associations. [TAF Prev Med Bull 2010; 9(6.000: 591-596

  12. O uso do Índice de Barthel Modificado em idosos: contrapondo capacidade funcional, dependência e fragilidade

    OpenAIRE

    Juliana Balbinot Reis Girondi; Karina Silveira de Almeida Hammerschimidt; Francisco Reis Tristão; Darla Lusia Ropelato Fernandez

    2014-01-01

    Introdução: Estudo transversal descritivo realizado entre junho e dezembro de 2010. Objetivo: avaliar a capacidade funcional de um grupo de idosos para conhecer e refletir sobre essa relação com a fragilidade e dependência. Métodos: Envolveu 18 idosos com deficiência física cadastrados em duas unidades de Saúde da Família de Florianópolis. Utilizou-se entrevista, aplicação do Mini Exame do Estado Mental e Índice de Barthel Modificado. Resultados: Aplicando-se estatística descritiva, identific...

  13. Polarización eléctrica de láminas delgadas de PbTi03 modificado

    OpenAIRE

    Ramos, P; Mendiola, J.

    1995-01-01

    Se comprueba experimentalmente, que la aplicación de un tratamiento eléctrico previo a las medidas eléctricas sobre las láminas delgadas de titanato de plomo modificado con calcio, obtenidas por el método de sol-gel, produce una mejora ostensible de sus propiedades ferroe-léctricas. El efecto se puede interpretar como un proceso de estabilización de la configuración de dominios a través de la redistribución de la carga espacial presente, especialmente vacantes de Pb y de O. Que los ciclos de ...

  14. Alimentos geneticamente modificados vs. saúde pessoal e ambiental: um estudo de opinião

    OpenAIRE

    Silva, Margarida; Frada, Mariana; Marques, Sandra; Santos, Ana; Santos, Luísa

    2004-01-01

    A comercialização de alimentos geneticamente modificados (OGM) desencadeou um debate social importante em toda a Europa a que Portugal não foi totalmente alheio. Embora a entrada de OGM na cadeia alimentar apresente a chancela governamental quanto à sua segurança sanitária e ambiental, a desconfiança dos consumidores europeus conduziu à efectiva eliminação dos mercados onde os OGM são sujeitos a rotulagem obrigatória que lhes acarreta visibilidade. Possivelmente tal postura terá sido de...

  15. Los Organismos Genéticamente Modificados: Opinión de un agrónomo

    OpenAIRE

    Durand, Jean-Louis

    2006-01-01

    Los organismos genéticamente modificados (OGM) inquietan: por ello se plantea investigar desde un punto de vista racional las consecuencias de su aparición para el conjunto de la sociedad. Una aproximación crítica a la cuestión de los OGM debe situarlos en el contexto general de la producción agrícola para el desarrollo sostenible. Los OGM combinan dos transformaciones profundas: una tecnológica, otra jurídico-comercial. Si la primera merece ser analizada con prudencia, es probablemente el as...

  16. 1300 MVA turbine generator for Ohi Nuclear Power Plant of the Kansai Electric Power Co

    International Nuclear Information System (INIS)

    Mitsubishi Electric Corp. has completed the manufacture of two largest capacity turbo-generators of 1300 MVA for Ohi nuclear power plants in Japan in accordance with the tide of making large size generators for nuclear power plants. The generator and the exciter are driven by being directly coupled with the 1175 MW steam turbine. It is a generator of 1800 RPM and cylindrical, revolving field type, with a hydrogen-cooled rotor, and the main exciter is of brushless, fully enclosed type. The stator of the generator is cooled directly with water. Considerable output increase and reliability improvement have been attempted. In addition, the following points have been considered: electrical loading, magnetic loading, electromagnetic vibration, stator coil, rotor shaft material and vibration. Characteristic test, temperature rise test and other engineering tests have been performed with thermocouples, search coils and strain gauges attached to about 1000 points, to measure temperature, vibration and magnetic flux of respective portions. In the synthetic test, the efficiency was 98.84% at rated turbine output and rated power factor. The temperature rise of stator winding was 33.6 deg C at maximum, its vibration was approximately 30 μm at maximum, and the coil surface strain in abrupt short test was about 1/6 of the breaking strain of the coil. These data indicate that the design is carried out with sufficient margin. It was also confirmed by the mechanical impedance measurement that the resonance point of the frame and bracket does not exist in the operating range. (Wakatsuki, Y.)

  17. A Clinical Study on Management of Incomplete Abortion by Manual Vacuum Aspiration (MVA

    Directory of Open Access Journals (Sweden)

    Arifa Akter Jahan

    2012-07-01

    Full Text Available Background: Abortion is an important social and public health issue. In Bangladesh complication from unsafe abortion is one of the leading causes of maternal mortality. It is a serious health problem. World Health Organisation estimates that 14% of maternal deaths which occur every year in the countries of South Asia including Bangladesh are due to abortion. Study shows manual vacuum aspiration procedure is safe and effective in incomplete abortion. Very few clinical trials were carried out in Bangladesh to assess the safety and effectivity of manual vacuum aspiration in managing incomplete abortion. Objective: To find out the outcome of manual vacuum aspiration in the management of patients of incomplete abortion. Materials and Methods: This observational descriptive study was conducted in the department of Obstetrics & Gynaecology, Dhaka Medical College & Hospital from June to December, 2004. One hundred cases of diagnosed incomplete abortion up to 12 weeks of gestation were managed by manual vacuum aspiration during this period. A data recording sheet was designed for this purpose. Haemodynamically stable patients with no history of induced abortion and fever were enrolled. Results: Procedure time of manual vacuum aspiration was short, average duration was 7 minutes. Bleeding was minimum (20-30 mL in 67% cases and weighted mean was 29.80 mL. Eighty three percent patients were stable during the procedure and only 3% needed blood transfusion. Nonnarcotic analgesics were used in 59% cases and 33% needed only proper counselling. Average duration of hospital stay was 2 hours. Effectiveness of the procedure was about 98% with very low post procedure complication rate (2%. Conclusion: MVA procedure is a safe and effective technique of uterine evacuation in incomplete abortion. It is quick, less expensive, effective and less painful. Hospital stay and chance of perforation of uterus is less. So this procedure should be considered by health care

  18. Procedimiento de síntesis de materiales piezoeléctricos de titanato circonato de plomo modificado con neodimio y mediante un procedimiento sol-gel.

    OpenAIRE

    Ayerbe de Aragón Aguilera, Asunción; Blanco Delgado, Carmen

    2001-01-01

    La invención tiene por objeto la síntesis de materiales piezoeléctricos de titanato-circonato de plomo (PZT), modificado con neodimio (PNZT) (entre 6 -10% moles de iones neodimio (Nd3+)), mediante procedimiento sol-gel modificado, determinando el valor exacto de las variables que intervienen en el proceso de preparación de los polvos precursores e innovando el proceso de secado de los polvos precursores mediante la introducción de la liofilización. Tras la calcinación y sinterización de los p...

  19. English-language needs in the probable work situations of the students of the Radio, Television, and Film Department at Ankara University

    OpenAIRE

    Şahin, Z Zeynep

    1994-01-01

    Ankara : Institute of Humanities and Letters of Bilkent Univ., 1994. Thesis (Master's) -- -Bilkent University, 1994. Includes bibliographical references leaves 63-65. This study was designed to investigate the fututre work-related English language needs of the students of the Radio, Television, and Film Department (RTFD) at the Faculty of Communication (FC) at Ankara University (AU). With this purpose, a needs analysis was conducted at Turkish Radio Television Corporation (TRT) whic...

  20. Live-Grid Operation and Maintenance of the 35 kV/121 MVA Superconducting Cable System

    Institute of Scientific and Technical Information of China (English)

    Huan-Huan Li; An-Lin Ren; Ying Xin; Hui Hong; Zhi-Li Chen; Lin-Na Shi

    2008-01-01

    A 33.5 m, 35 kV/121 MVA, three-phase, warm dielectric HTS power cable system was successfully installed and activated in China Southern Power Grid at the Puji substation in Kunming on April 19th of 2004, supplying electricity to four industrial customers (including two metallurgical refineries) and a residential population of about 100000. In this paper, we give an update on the operation and maintenance status of the system and comments on reliability issues. We conclude that the superconducting cable system is currently quite robust and feasible for particular utility applications, and it will be improved by advancement in cryogenic equipment and system technology.

  1. Laceração canicular: a utilização do bastão de Veirs modificado

    Directory of Open Access Journals (Sweden)

    Silvia Helena Tavares Lorena

    2013-12-01

    Full Text Available Os autores descrevem a utilização do bastão de Veirs modificado como modelador intracanalicular, facilitando a sutura do canalículo lacrimal e evitando a sua estenose. A técnica consiste na introdução do bastão de Veirs modificado no lúmen canalicular pelo ponto lacrimal, em direção ao saco lacrimal. A seguir as terminações distal e proximal do canalículo lesado são identificadas e a região lateral e medial da mucosa canalicular são suturadas boca a boca, com pontos separados de polivicryl 8.0, deixando bem coaptadas as bordas da ferida, ao redor do bastão. A reconstrução da ferida é completada por planos. O fio de mononylon 6.0 do bastão que se exterioriza pelo ponto lacrimal será fixado à pele da pálpebra em ângulo reto. O bastão permanece por 4 a 6 semanas no lúmen canalicular.

  2. The Rates of Undergoing Cancer Screening Among Family Physicians Aged 40 and Older Working in Ankara Province

    OpenAIRE

    Ayşe Selda Tekiner; Gülseren Lale; A. Gülsen Ceyhun Peker2

    2016-01-01

    Aim: Family physicians, as role models for their patients, self health behaviors are important. We aimed to investigate the rate of undergoing cancer screening among family physicians. Methods: This is a descriptive and questionnaire-based study. Although we aimed to interview 1100 family physicians aged 40 or older working at family health centers of Ankara, the study was performed with 453 physicians. The questionnaire form comprised of the doctors’ opinions about cancer s...

  3. Longhorned Beetles Collection of the Entomology Museum of Central Anatolia Forestry Research Directorship, Ankara, Turkey (Coleoptera,Cerambycidae)

    OpenAIRE

    ÖZDİKMEN, Hüseyin; ŞAHİN, Özlem

    2010-01-01

    In the present paper, specimens of Cerambycidae were examined in the Entomology Museum of Central Anatolia Forestry Research Directorship, Ankara, Turkey. As a result of identification of these specimens, thirty-eight species and two subspecies belonging to twenty-eight genera of five subfamilies were determined. With this paper, new faunistic data and some zoogeographical evaluations were presented on longhorned beetles fauna (Coleoptera, Cerambycidae) of Turkey. The faunistic data in the pr...

  4. A Study on the Anthropometric Measures, Breast feeding and Nutrition Status of Children of 0 36 Months Living in Ankara

    OpenAIRE

    ŞANLIER, Nevin; AYTEKİN, Fulya

    2004-01-01

    Various diseases can be prevented at an early period by using anthropometric measures to determine undernourishment and malnutrition and to follow growth in children. This study was planned and implemented to examine the physical development of children of 0-3 ages. 679 children applying to 15 Mother and Child Health and Family Planning Centers and District Health Centers in different quarters of Ankara were studied. Midwives and nurses working in these centers properly measured heights ...

  5. The Relationship between Market Orientation, Innovation Orientation and Firm Performance: A Research in Four and Five Star Hotels in Ankara

    OpenAIRE

    ERDEM, Barış; Kudret GÜL; Melike GÜL

    2013-01-01

    In this study, relationships between market orientation, innovation orientation and firm performance were researched. As samples, four and five star hotel enterprises in Ankara were selected. Research data were obtained from senior managers of 40 hotel enterprises by using questionnaire technique. According to findings, it was detected that market orientation and innovation orientation have significant and positive oriented relations with firm performance. A significant and positive orien...

  6. Water utilization of vegetables grown under plastic greenhouse conditions in Ankara using neutron probe technique

    International Nuclear Information System (INIS)

    In order to find suitable varieties of tomato, pepper and cucumber for plastic greenhouse conditions in Ankara and ensure both higher yields and lower NO3 leaching greenhouse experiments were conducted for three years. In the first year (2001) of the experiment four different varieties from each vegetable, namely, Tomato (Ecem F1, 9920 F1, 2116 F1 and Yazg1 F1), Cucumber (Hizir F1 , Rapido, Hana, and Luna) and Pepper (1245 F1, 730 F1, Serademre 8 and 710 F1) had been grown in the plastic greenhouse using drip irrigation-fertilization system. Yazg1 F1 variety for tomato, Hizir F1 variety for cucumber and Serademre 8 variety for pepper were chosen to be suitable varieties to grow in the plastic greenhouse conditions in Ankara. One access tube in each N3 and N0 treatment plots of tomato, cucumber and pepper in 2002 and 2003 experiments were installed for the soil moisture determinations at 30, 60 and 90 cm depths. Readings with the neutron probe were taken before planting and after harvest for the water consumption calculations using the water balance approach and the WUE was calculated on the basis of the ratio of dry matter weight to the amount of water consumed. Tensiometer and suction cups were installed at 15, 30, 45 and 60 cm depths only to N1, N2 and N3 treatments plots of each vegetable in 2002 and 2003. Tensiometer readings were taken just before irrigation. Also, soil solution samples from suction cups were taken at final harvest and NO3 determinations were done with RQFLEX nitrate test strips. Significantly higher yields and WUE values were obtained when the same amount of N fertilizer is applied through fertigation compared to the treatment where N fertilizer applied to the soil then drip irrigated. The nitrate concentrations of the soil solution increased as the N rates increased and no NO3 had been found in the soil solution taken from 75 cm soil depth, indicating that no leaching of N fertilizer occurred beyond 75 cm soil depth. (author)

  7. A proton irradiation test facility for space research in Ankara, Turkey

    Science.gov (United States)

    Gencer, Ayşenur; Yiǧitoǧlu, Merve; Bilge Demirköz, Melahat; Efthymiopoulos, Ilias

    2016-07-01

    Space radiation often affects the electronic components' performance during the mission duration. In order to ensure reliable performance, the components must be tested to at least the expected dose that will be received in space, before the mission. Accelerator facilities are widely used for such irradiation tests around the world. Turkish Atomic Energy Authority (TAEA) has a 15MeV to 30MeV variable proton cyclotron in Ankara and the facility's main purpose is to produce radioisotopes in three different rooms for different target systems. There is also an R&D room which can be used for research purposes. This paper will detail the design and current state of the construction of a beamline to perform Single Event Effect (SEE) tests in Ankara for the first time. ESA ESCC No.25100 Standard Single Event Effect Test Method and Guidelines is being considered for these SEE tests. The proton beam kinetic energy must be between 20MeV and 200MeV according to the standard. While the proton energy is suitable for SEE tests, the beam size must be 15.40cm x 21.55cm and the flux must be between 10 ^{5} p/cm ^{2}/s to at least 10 ^{8} p/cm ^{2}/s according to the standard. The beam size at the entrance of the R&D room is mm-sized and the current is variable between 10μA and 1.2mA. Therefore, a defocusing beam line has been designed to enlarge the beam size and reduce the flux value. The beam line has quadrupole magnets to enlarge the beam size and the collimators and scattering foils are used for flux reduction. This facility will provide proton fluxes between 10 ^{7} p/cm ^{2}/s and 10 ^{10} p/cm ^{2}/s for the area defined in the standard when completed. Also for testing solar cells developed for space, the proton beam energy will be lowered below 10MeV. This project has been funded by Ministry of Development in Turkey and the beam line construction will finish in two years and SEE tests will be performed for the first time in Turkey.

  8. [Atmospheric concentration of fungus spores in Ankara and the effect of meteorological factors in 2003 period].

    Science.gov (United States)

    Ceter, Talip; Pinar, Nur Münevver

    2009-10-01

    The atmospheric concentrations of airborne fungus spores change continuously according to the meteorological factors, and their intensity have important allergic effects on atopic subjects and opportunistic pathogenic effects on immunocompromised patients. The aim of this study was to identify the fungal spores found in Ankara atmosphere during 2003 period and to investigate the changes in spore concentrations in relation to meteorological factors. Fungal spores were sampled by using 7-day Burkard volumetric trap between January to December 2003, and probable identification was performed microscopically based on their morphological structures. A total of 433.079 spores/m3 belonging to 35 taxa were observed during the study. The rates of these taxa were as follows; 75.5% Cladosporium, 6.1% Alternaria, 2.2% Leptosphaeria, 2.2% Ustilago, 2.1% 1-septate ascospores, 2% Exosporium, 1.6% Pleospora, and 1.3% Drechslera. The other taxa with concentrations Peronospora, Venturia, Paraphaeosphaeria, Epicoccum, Didymella, Chaetomium and Fusarium rates between 0.7-0.1%; Oidium, Xylaria, Botrytis, Melanospora, Dictyosporium, Sporormiella and Tetracoccosporium rates between 0.09-0.01%). Although fungal spores were detected in all months in Ankara atmosphere, the evaluation of the seasonal distribution of spore concentrations revealed that the highest value was detected in July (100.697 spores/m3), while the lowest value was in January (4268 spores/m3). When the effects of meteorological factors on spore concentrations were investigated, it was found that, monthly mean temperature (> 20 degrees C) has a strong positive correlation (p < 0.01), and monthly mean relative humidity (< %50) and precipitation (0-20 mm) have strong negative correlations (p < 0.01) on the spore concentrations, while wind velocity (3 m/s) has a slightly positive effect. An annual spore calendar which indicated weekly concentrations and allergenicity levels of those identified fungal spores, was also prepared

  9. Immunotherapy with MVA-BN®-HER2 induces HER-2-specific Th1 immunity and alters the intratumoral balance of effector and regulatory T cells

    OpenAIRE

    Mandl, Stefanie J; Rountree, Ryan B; Dalpozzo, Katie; Do, Lisa; Lombardo, John R.; Schoonmaker, Peter L.; Dirmeier, Ulrike; Steigerwald, Robin; Giffon, Thierry; Laus, Reiner; Delcayre, Alain

    2011-01-01

    MVA-BN®-HER2 is a new candidate immunotherapy designed for the treatment of HER-2-positive breast cancer. Here, we demonstrate that a single treatment with MVA-BN®-HER2 exerts potent anti-tumor efficacy in a murine model of experimental pulmonary metastasis. This anti-tumor efficacy occurred despite a strong tumor-mediated immunosuppressive environment characterized by a high frequency of regulatory T cells (Treg) in the lungs of tumor-bearing mice. Immunogenicity studies showed that treatmen...

  10. Separation of hydrograph components using stable isotopes case study: Guvenc basin, Ankara

    International Nuclear Information System (INIS)

    In this paper, a stable environmental isotope study was carried out from analysis of water samples collected from rainfall, runoff (total discharge), springs (subsurface flows), and wells (groundwater) between 1996-2000. The research site was Guvenc basin located near Yenimahalle-Ankara having a drainage area of about 16.125 km2 . There are many representative hydrologic basins established in Turkey by the General Directorate of Rural Services (GDRS). In these basins, the precipitation and runoff values are recorded continuously and relationship between these values are also investigated in the last ten-twenty years. Guvenc basin, the study area, is one of these representative basins. On this basin, a stream gaging, station and five rain gages have been installed to collect and analyze runoff and rainfall data. A small dam was constructed at the outlet of the basin to store and provide water for distribution to agricultural areas. The characteristics of precipitation data are collected at raingage station, installed near the runoff measuring. To monitor the groundwater isotopic composition regular samples are also collected from a dug well near runoff station

  11. Analysis of Ankara urban area from human bioclimatologic point of view

    Directory of Open Access Journals (Sweden)

    Necla Türkoğlu

    2011-10-01

    Full Text Available In this study, the Physiological Equivalent Temperatures (PET of different land patterns in the Ankara urban area has been analyzed. The spatial distribution and temporal variation of the thermal perceptions and the grades of thermal stress caused by the thermal conditions have been determined for 00:00, 03:00, 06:00, 09:00, 12:00, 15:00, 18:00 and 21:00 hours during the December and July of 2010. The effects of physiographic features such as elevation, aspect, slope, and especially land use, on the PETs have also been examined. According to the analysis which is carried out by using PET thermal perception index, in the research area the thermal comfort values varies around from -6 to -11°C in winter and from 13°C to 50°C in summer. In both periods the open spaces (grasslands of the city forests have the maximum PET values during the day. Either in December and in July the urban area (city center has and cool island during the day and has a heat island during the night.

  12. Consumer Decision - Making Process in E - Commerce: Case of Salzburg - Ankara

    Directory of Open Access Journals (Sweden)

    Ahmet Gürbüz

    2015-09-01

    Full Text Available In a globalized world, companies need to track the changes occur in their internal and external stakeholders in order to develop consistent strategies, structures and systems for success in e-commerce. Tracking and researching the existing and potential customers and companies as external stakeholders has become very important. This study aims to reveal a scientific approach about the purchasing decisions of e-commerce companies’ customers, to carry the e-commerce applications in Austria to Turkey, to transfer the e-commerce applications in Turkey to Austria and to contribute for the development of e-commerce. To achieve this, in-depth interviews were conducted with 16 female e-commerce users in Ankara/Turkey and in Salzburg/Austria to reveal the similarities and differences between the purchasing decision processes of different countries’ citizens. Similarities and differences in the purchasing decision process of customers were determined for the dimensions of price compatibility, practicality, accessibility, speed, comfort, convenience and cost and suggestions are offered for the development of e-commerce in Turkey.

  13. Consumers’ Determination of Red Meat and Meat Products Purchase Behaviour – City of Ankara Sample

    Directory of Open Access Journals (Sweden)

    Hasan Arısoy

    2014-08-01

    Full Text Available Nowadays, meat consuming level is an indicator of development of countries. The reason for this is the importance of animal proteins such as meat, milk and egg in human nutrition. People in Turkey do not get enough animal protein. Increase of meat and meat related product prices is effective on this. The purpose of this study is to bring up the purchase and consume behavior of consumers. Surveys completed in urban areas around city of Ankara are used primarily. Using Main Mass Ratio Based Simple Occurrence Probability Sampling method 338 families were interviewed. Completed surveys were separated into 3 groups; high, middle, low. As a result of the research, expense for food in total income is around %34. This ratio for families with low income is %53, for families with middle income is %35 and for families with high income is %33. It is found that as income levels of consumers raised, food expenses raise as well. But it shows that as income level increases, expense ratio for food decreases. Food reliability is the most effective factor on consumers’ decision of purchase. Studies show doubts of consumers about food reliability. It is understood that legal adjustments are not applied enough. Especially tight food inspections would be positive on consumer behavior.

  14. On-going research projects at Ankara Nuclear Research Center in Agriculture and Animal Science

    International Nuclear Information System (INIS)

    Full text: The research and development activities of Ankara Nuclear Research Center in Agriculture and Animal Science(ANRCAA) are concentrated on the contribution of atomic energy to peace by the use of nuclear and related techniques in food, agriculture and animal science. Nuclear techniques are used in the above fields in two ways: in vitro or in vivo radio tracing the substances and processes of biological importance, and irradiation of biological materials for preservation and quality modification. Research projects are carried out by interdisciplinary studies with well equipped laboratories at the Center. The projects in progress conducted by the Center comprises nuclear-aided researches in soil fertility, plant nutrition, plant protection, improvement of field crops, improvement of horticultural plants and forest trees by mutation breeding, in vitro culture technique with mutagen treatments, use of phosphogypsum in soil amelioration, sterilization of medical supplies, wastewater treatment, animal nutrition, animal health and productivity and accreditation. The on-going projects with the above subjects will be summarized for possible collaborations

  15. Seasonal variation of surface temperature based on land cover in Ankara

    Directory of Open Access Journals (Sweden)

    İhsan Çiçek

    2013-03-01

    Full Text Available In this study, the seasonal variation of the surface temperature of Ankara urban area and its enviroment have been analyzed by using Landsat 7 image. The Landsat 7 images of each month from 2007 to 2011 have been used to analyze the annually changes of the surface temperature. The land cover of the research area was defined with supervised classification method on the basis of the satellite image belonging to 2008 July. After determining the surface temperatures from 6-1 bands of satellite images, the monthly mean surface temperatures were calculated for land cover classification for the period between 2007 and 2011. Accordşng to the results obtained, the surface temperatures are high in summer and low in winter from the air temperatures. all satellite images were taken at 10:00 am, it is found that urban areas are cooler than rural areas at 10:00 am. Regarding the land cover classification, the water surfaces are the coolest surfaces during the whole year. The warmest areas are the grasslands and dry farming areas. While the parks are warmer than the urban areas during the winter, during the summer they are cooler than artificial land covers. The urban areas with higher building density are the cooler surfaces after water bodies.

  16. Researches on Hygienic Qualities of Airan and Yoghurt Samples Sold in Ankara Markets (in Turkish)

    OpenAIRE

    Duru, Suna; Özgüneş, Hilâl

    1981-01-01

    Birçok ülkede süt ve süt ürünleri kişinin diyetinin önemli bir bölümünü oluşturmaktadır. Bu gıda maddelerinin hastalık taşıma potansiyeli yüksek olduğundan, hijyenik kalitelerine özen gösterilmesi gerekir. Bu çalışmada, yurdumuzda büyük ölçüde tüketilen ürünlerden ayran ve yoğurt, hijyenik kaliteleri yönünden incelendi. 9 Haziran-14 Ağustos 1979 tarihleri arasında çeşitli zamanlarda Ankara piyasasından toplanan 50 ayran ve 20 yoğurt örneğinde koliform bakteri sayımı, 40 ayran ve 20 yoğurt ö...

  17. THE EXPECTANCIES OF THE HEALTH SECTOR FROM ACCOUNTING EDUCATION AND THE IMPLEMENTATION OF ANKARA PUBLIC HOSPITALS

    Directory of Open Access Journals (Sweden)

    Seyhan ÇİL KOÇYİĞİT

    2016-01-01

    Full Text Available Health sector requires qualified accounting personnel and demands qualified accounting personnel who knows the sector and who has the vocational knowledge and the ability. Education and training activities designed to meet the expectencies and the requirements of the sector are very important. These requirements can be met by the good quality of accounting education designed by the demands of the health sector. In this research, a survey has been administered to the managers of Ankara public hospitals in order to reveal the expectencies of the health sector from accounting education. The results of the survey show the follwings; accounting education should include practical information, The Uniform Chart Of Accounts should be developed in order to meet the expectencies of the health sector, there should be an internship opportunity at the health sector for accounting students, the content of the accounting courses should be determined by a cooperation between the sector and the academia and the demands of the health sector should be taken into consideration more.

  18. On-going research projects at Ankara Nuclear research center in agriculture and animal science

    International Nuclear Information System (INIS)

    Full text:The research and development activities of Ankara Nuclear Research Center in Agriculture and Animal Science(ANRCAA) are concentrated on the contribution of atomic energy to peace by the use of nuclear and related techniques in food, agriculture and animal science. Nuclear techniques are used in the above fields in two ways: in vitro or in vivo radio tracing the substances and processes of biological importance, and irradiation of biological materials for preservation and quality modification. Research projects are carried out by interdisciplinary studies with well equipped laboratories at the Center. The projects in progress conducted by the Center comprises nuclear-aided researches in soil fertility, plant nutrition, plant protection, improvement of field crops, improvement of horticultural plants and forest trees by mutation breeding, in vitro culture technique with mutagen treatments, use of phosphogypsum in soil amelioration, sterilization of medical supplies, wastewater treatment, animal nutrition, animal health and productivity and accreditation. The on-going projects with the above subjects will be summarized for possible collaborations

  19. The main activities and scientific collaboration possibilities at Ankara Nuclear research and training center

    International Nuclear Information System (INIS)

    Full text: Founded in 1964, Ankara Nuclear Research and Training Center (ANRTC) conducts and facilitates the scientific activities including training (summer practice, MSc and Ph D studies in physics and chemistry, IAEA fellowship programs etc.), research and other studies in nuclear and related fields. As it's a part of main duties, ANRTC has analysis on the variety of samples, and radiation protection services commercially, for radiation workers in state, public and private sectors. Research, development and application projects implemented in this Center have mostly been supported by State Planning Organization (SPO) and Turkish Atomic Energy Authority (TAEA). In addition to the projects there are on going collaborative studies with some national Universities and International Atomic Energy Agency. The main activities carried out in ANRTC can be summarized as: studies on experimental nuclear physics, application of nuclear techniques such as XRF, XRD, Gamma, Alpha, etc. for environmental pollutants, archaeological and geological dating, elemental and crystal structural analyses, studies on the detection of irradiated foodstuff by ESR, development of accident dosimeters to be used in the case of a nuclear or radiological accident, and radiation matter interaction studies. In near future, for young scientists, there will be new collaboration possibilities related to accelerator-based applications, especially the new production methods of radioisotopes and their radiopharmaceuticals by using a cyclotron when our 30 MeVp / 15MeVd cyclotron facility project is underway

  20. Erasmus Öğrenci Hareketliliği: Ankara Üniversitesi Değişim Öğrencilerinin Görüşlerine Göre Bazı İyi Uygulamalar

    OpenAIRE

    ASLAN, Berna; Jacobs, Dürdane Bayram

    2012-01-01

    In this case study, opinions of Ankara University Erasmus mobility students were analyzed about their experiences at host Erasmus universities. The aim of the study is to learn the experiences of Ankara University mobility students, find out the reasons of attending Erasmus mobility, the good examples they experienced and discuss whether it changes according to a host country. It is a case study examining the views of Ankara University Erasmus mobility students. A case study research method i...

  1. Transparent 3D Visualization of Archaeological Remains in Roman Site in Ankara-Turkey with Ground Penetrating Radar Method

    Science.gov (United States)

    Kadioglu, S.

    2009-04-01

    Transparent 3D Visualization of Archaeological Remains in Roman Site in Ankara-Turkey with Ground Penetrating Radar Method Selma KADIOGLU Ankara University, Faculty of Engineering, Department of Geophysical Engineering, 06100 Tandogan/ANKARA-TURKEY kadioglu@eng.ankara.edu.tr Anatolia has always been more the point of transit, a bridge between West and East. Anatolia has been a home for ideas moving from all directions. So it is that in the Roman and post-Roman periods the role of Anatolia in general and of Ancyra (the Roman name of Ankara) in particular was of the greatest importance. Now, the visible archaeological remains of Roman period in Ankara are Roman Bath, Gymnasium, the Temple of Augustus of Rome, Street, Theatre, City Defence-Wall. The Caesar Augustus, the first Roman Emperor, conquered Asia Minor in 25 BC. Then a marble temple was built in Ancyra, the administrative capital of province, today the capital of Turkish Republic, Ankara. This monument was consecrated to the Empreror and to the Goddess Rome. This temple is supposed to have built over an earlier temple dedicated to Kybele and Men between 25 -20 BC. After the death of the Augustus in 14AD, a copy of the text of "Res Gestae Divi Augusti" was inscribed on the interior of the pronaos in Latin, whereas a Greek translation is also present on an exterior wall of the cella. In the 5th century, it was converted in to a church by the Byzantines. The aim of this study is to determine old buried archaeological remains in the Augustus temple, Roman Bath and in the governorship agora in Ulus district. These remains were imaged with transparent three dimensional (3D) visualization of the ground penetrating radar (GPR) data. Parallel two dimensional (2D) GPR profile data were acquired in the study areas, and then a 3D data volume were built using parallel 2D GPR data. A simplified amplitude-colour range and appropriate opacity function were constructed and transparent 3D image were obtained to activate buried

  2. Cultivos genéticamente modificados: las promesas y las buenas intenciones no bastan

    Directory of Open Access Journals (Sweden)

    Jaime E García G

    2007-06-01

    Full Text Available Se refutan y cuestionan los razonamientos expuestos por las autoras en el artículo "Relación de los cultivos modificados genéticamente con el ambiente y la salud de la población costarricense" publicado en esta revista por Espinoza et al. (52: 727-732, 2004. Para ello se contraponen éstos con las evidencias expuestas y analizadas tanto por científicos como por organizaciones nacionales e internacionales independientes de diverso tipo en diferentes lugares del mundo (e.g. Organización Mundial de la Salud, internacional de Consumidores, Médicos y Científicos por una Aplicación Responsable de la Ciencia y la Tecnología, Unión internacional para la Conservación de la Naturaleza, Consejo Universitario de la Universidad de Costa Rica y el Panel de Ciencias independiente, que confirman la incertidumbre y limitaciones actuales de la ciencia en esta área, así como de los mecanismos de bioseguridad propuestos y puestos en vigor. En materias tan importantes y básicas para la supervivencia como las que nos ocupan -el ambiente, la biodiversidad y la seguridad alimentaria- no es prudente apostar a promesas basadas en la misma lógica promocionada desde hace poco más de cinco décadas por la denominada "revolución verde". Es necesario continuar este debate basado en el análisis holístico de los hechos, así como en razonamientos éticos, evitando caer en posiciones emocionales que llegan a confundir la realidad virtual con la verdadera realidad.Genetically modified crops: promises and good intentions are not enough (refutation to Espinoza et al. 2004, Rev. Biol. Trop. 52 (3: 727-732. The arguments presented by Espinoza et al. in their paper "Relationship of genetically modified crops with the environment and health of the Costa Rican human population" published in this journal (Rev. Biol. Trop. 52: 727-732, 2004 are questioned and refuted. The arguments are confronted with evidence offered by scientists and national and international

  3. Os organismos geneticamente modificados e a rotulagem dos alimentos - uma análise a partir dos direitos dos consumidores

    Directory of Open Access Journals (Sweden)

    Marcello Vitor Rocha Cota

    2015-11-01

    Full Text Available Ao longo do presente trabalho serão analisados os organismos geneticamente modificados (OGMs frente aos riscos à saúde alimentar e ao meio ambiente, sobretudo o princípio da precaução e a rotulagem dos produtos. A partir de uma dimensão comparativa de três realidades distintas, isto é, a estadunidense, a europeia, com ênfase mercado ao português, e a brasileira – sob um prisma ambiental, a rotulagem dos produtos será confrontada com os direitos dos consumidores, embora em determinadas ocasiões, diante da inexistência de informações adequadas e claras dos produtos, que estão sendo ofertados e consumidos, os consumidores não sabem o que estão consumindo.

  4. Avaliação de risco dos organismos geneticamente modificados Risk assessment of genetically modified organisms

    Directory of Open Access Journals (Sweden)

    Thadeu Estevam Moreira Maramaldo Costa

    2011-01-01

    Full Text Available Desde o começo de sua comercialização, em 1996, a área global de plantações transgênicas aumentou mais de cinquenta vezes. Nas duas últimas décadas, organizações governamentais e intergovernamentais têm planejado estratégias e protocolos para o estudo da segurança de alimentos derivados de cultivos geneticamente modificados. Os testes de segurança são realizados caso a caso e conduzidos de acordo com as características específicas das culturas modificadas e as mudanças introduzidas através da modificação genética, levando em conta o conceito de equivalência substancial. No presente trabalho, estão relatadas algumas abordagens de avaliação de risco de alimentos geneticamente modificados, assim como alguns problemas relacionados à construção genética ou mesmo à expressão do gene inseridoSince the commercial approve in 1996, the global area of transgenic crops has raised more than 50 times. In the last two decades, governments have been planning strategies and protocols for safety assessment of food and feed genetically modified (GM. Evaluation of food safety should be taken on a case-by-case analysis depending on the specific traits of the modified crops and the changes introduced by the genetic modification, using for this the concept of substantial equivalence. This work presents approaches for the risk assessment of GM food, as well as some problems related with the genetic construction or even with the expression of the inserted gene

  5. Immunotherapy with MVA-BN®-HER2 induces HER-2-specific Th1 immunity and alters the intratumoral balance of effector and regulatory T cells.

    Science.gov (United States)

    Mandl, Stefanie J; Rountree, Ryan B; Dalpozzo, Katie; Do, Lisa; Lombardo, John R; Schoonmaker, Peter L; Dirmeier, Ulrike; Steigerwald, Robin; Giffon, Thierry; Laus, Reiner; Delcayre, Alain

    2012-01-01

    MVA-BN®-HER2 is a new candidate immunotherapy designed for the treatment of HER-2-positive breast cancer. Here, we demonstrate that a single treatment with MVA-BN®-HER2 exerts potent anti-tumor efficacy in a murine model of experimental pulmonary metastasis. This anti-tumor efficacy occurred despite a strong tumor-mediated immunosuppressive environment characterized by a high frequency of regulatory T cells (T(reg)) in the lungs of tumor-bearing mice. Immunogenicity studies showed that treatment with MVA-BN®-HER2 induced strongly Th1-dominated HER-2-specific antibody and T-cell responses. MVA-BN®-HER2-induced anti-tumor activity was characterized by an increased infiltration of lungs with highly activated, HER-2-specific, CD8+CD11c+ T cells accompanied by a decrease in the frequency of T(reg) cells in the lung, resulting in a significantly increased ratio of effector T cells to T(reg) cells. In contrast, administration of HER2 protein formulated in Complete Freund's Adjuvant (CFA) induced a strongly Th2-biased immune response to HER-2. However, this did not lead to significant infiltration of the tumor-bearing lungs by CD8+ T cells or the decrease in the frequency of T(reg) cells nor did it result in anti-tumor efficacy. In vivo depletion of CD8+ cells confirmed that CD8 T cells were required for the anti-tumor activity of MVA-BN®-HER2. Furthermore, depletion of CD4+ or CD25+ cells demonstrated that tumor-induced T(reg) cells promoted tumor growth and that CD4 effector cells also contribute to MVA-BN®-HER2-mediated anti-tumor efficacy. Taken together, our data demonstrate that treatment with MVA-BN®-HER2 controls tumor growth through mechanisms including the induction of Th1-biased HER-2-specific immune responses and the control of tumor-mediated immunosuppression. PMID:21822917

  6. Geochemistry and Geochronology of Oceanic Rock Assemblages in the Izmir-Ankara-Erzincan Suture Zone, Turkey

    Science.gov (United States)

    Sarifakioglu, Ender; Dilek, Yildirim; Sevin, Mustafa

    2013-04-01

    We report here new data from the Izmir-Ankara-Erzincan Suture Zone (IAESZ), which connects the Vardar Suture Zone in the west with the Sevan-Akera Suture Zone in the east. It occurs between the Sakarya Continent and the Anatolide-Tauride ribbon continent in Turkey, and consists mainly of ophiolitic thrust sheets emplaced into a subduction-accretion mélange. The ophiolites include upper mantle peridotites, cumulate to massive gabbros, sheeted doleritic dykes, plagiogranite dykes and stocks, and basalt-chert-radiolarite as in a complete Penrose-type ophiolite pseudostratigraphy. They display a geochemical progression in their magmatic evolution from initially MORB-like to island arc tholeiites (IAT) to boninites, similar to many other Tethyan ophiolites in the eastern Mediterranean region. Boninitic dykes and volcanic rocks have very low TiO2 (0.03-0.50 wt.%), Nb (0.03-1.9 ppm), Y (1,9-10.7ppm) and Zr (4.10-42 ppm) values. IAT-type doleritic dykes and basaltic lavas contain low TiO2 (0.42-1.04 wt.%), Nb (0.90-2.7 wt.%), Y (9.7-33.3 ppm) and Zr (12.7-85 ppm). The TiO2 (0.81-2.60 wt.%), Nb (3.30-4.8 ppm), Y (22.6-63.8 ppm) and Zr (36.01-179.33 ppm) contents of the MORB-like basaltic rocks are higher than those in the IAT and boninitic lavas. The subduction-accretion mélange (Ankara Mélange) in the central part of the IAESZ, includes volcanic mega-blocks derived from seamounts and/or an oceanic plateau that have OIB and P-MORB geochemical characteristics. This subduction-accretion complex also contains blocks of MORB- and/or IAT-like ophiolite fragments within a matrix composed of serpentinite, altered volcanics-volcaniclastics and/or pelitic sedimentary rocks. Pegmatitic gabbros in the ophiolites in the western and eastern IAESZ reveal 40Ar-39Ar hornblende ages of 168.9Ma and 195.7±2.7Ma, respectively. Plagiogranite dykes in the central and eastern parts of the IAESZ are 180 Ma and 179±2 Ma to 180.1±2.4 Ma in age as determined from U-Pb from zircon dates. The

  7. The impact of anti-smoking laws on high school students in Ankara, Turkey

    Directory of Open Access Journals (Sweden)

    Melike Demir

    2015-12-01

    Full Text Available ABSTRACT OBJECTIVE: To determine the factors affecting the smoking habits of high school students, their thoughts about changes resulting from anti-smoking laws, and how they are affected by those laws. METHODS: In this cross-sectional study, 11th-grade students at eight high schools in Ankara, Turkey, were invited to complete a questionnaire. RESULTS: A total of 1,199 students completed the questionnaire satisfactorily. The mean age of the respondents was 17.0 0.6 years; 56.1% were female, of whom 15.3% were smokers; and 43.9% were male, of whom 43.7% were smokers (p < 0.001. The independent risk factors for smoking were male gender, attending a vocational school, having a sibling who smokes, having a friend who smokes, and poor academic performance. Of the respondents, 74.7% were aware of the content of anti-smoking laws; 81.8% approved of the restrictions and fines; and 8.1% had quit smoking because of those laws. According to the respondents, the interventions that were most effective were the (television broadcast of films about the hazards of smoking and the ban on cigarette sales to minors. The prevalence of smoking was highest (31.5% among students attending vocational high schools but lowest (7.5% among those attending medical vocational high schools. Although 57.1% of the smokers were aware of the existence of a smoking cessation helpline, only 3.7% had called, none of whom had made any attempt to quit smoking. CONCLUSIONS: Although most of the students evaluated were aware of the harmful effects of smoking and approved of the anti-smoking laws, only a minority of those who smoked sought professional help to quit.

  8. Data evaluation of Ankara Numune Training and Education Hospital immunofixation electrophoresis

    Directory of Open Access Journals (Sweden)

    Müjgan Ercan

    2013-06-01

    Full Text Available Objective: The immunofixation electrophoresis (IFE is a method used for the diagnosis, monitoring and treatment of monoclonal gammopathies (MG. The monoclonal gammopathies include premalignant or malignant disease such as multiple myeloma, Waldenström’s makroglobulinaemia (WM, plasmacytoma, monoclonal gammopati of undetermined significance (MGUS, plasma cell leukemia and amiloidosis (AL. We aimed to evaluate IFE reports analyzed in Ankara Numune Teaching and Research hospital from January to July 2011.Methods: IFE was studied by Interlab G26 in our laboratory. In this study, all IFE reports examined which analyzed between January and July 2011. The results assessed for frequency and type of MG and also for diagnosis of the disease. Results: A total of 688 patients from January to July 2011 were studied by IFE and 126 (18.3% patients have been identified as MG. There were 72 (57.1% men and 54 (42.9% women in patients with MG. Distribution of isotypes were IgG kappa 41.3%, IgG lambda 35.6%, IgA kappa 11.5%, IgA lambda 5.8%, IgM kappa 3.8% and IgM lambda 1.9%. There were MM (65.9%, WM (0.07% and MGUS (33% in patients with MG.Conclusion: IgG kappa was the most common type of MG and MM was the most common disease in these patients. MG detection rate was 18% in patients requested IFE test. The decreased rate has shown that at first serum protein electrophoresis must studied and then IFE test must be performed in patients who have an uncertainty of MG. J Clin Exp Invest 2013; 4 (2:148-152Key words: Monoclonal gammopathies, immunofixation electrophoresis, multiple myeloma

  9. The Experiences of Violence and Occupational Health Risks of Sex Workers Working in Brothels in Ankara

    Directory of Open Access Journals (Sweden)

    Yaşar Bilge

    2012-06-01

    Full Text Available Objective: The aim of this study was to reveal and discuss occupational health risks, violence against sex workers working in brothels and their working conditions in Ankara. Materials and Methods: The study included 138 sex workers. Data were collected at face to face interviews with a questionnaire composed of 40 questions about socio-demographic features, familial characteristics, reasons for becoming a sex worker, experiences of violence and occupational health risks. Results: Twenty-two point five percent of the women were aged 21-30 years and 39.9% were aged 31-40 years. The mean time of education was 5.9±3.5 (0-14 years. Forty-eight point five percent of the women were exposed to physical abuse and 13% of the women had been exposed to sexual abuse in their childhood. Fifty-five point eight percent of the women reported that their clients always used condoms, but 97.1% of the women noted that their clients insisted on not using a condom. Fourteen point five percent and 70.3% of the women were exposed to physical and verbal violence respectively from their clients. Ten point one percent of the women suffered sexual assault while working. Conclusion: Sex workers, like other people, should have human rights, all types of violence that they face should be eliminated and the social conditions they are exposed to should be improved. Sexually transmitted diseases, the most important health risk of sex workers, should be considered as occupational diseases in the new regulations.

  10. Ankara Üniversitesi Sağlık Bilimleri Fakültesi Kütüphaneleri’nde Kataloglamada Uyumun Ölçülmesi / Measuring Cataloging Consistency in Health Sciences Faculty Libraries at Ankara University

    Directory of Open Access Journals (Sweden)

    Doğan Atılgan

    2006-04-01

    Full Text Available The purpose of this study was to examine the cataloging consistency between health sciences faculty libraries at Ankara University. Online catalogs at four faculty libraries are used to detect differencies in MARC records. Access points included AACR2, main entry, title statement and area of responsibility, edition statement, publication, distribution (etc., physical description area, series area, and added entries are compared. Findings showed certain amount of inconsistency among the access points analysed. Highest numbers of inconsistencies among all libraries were found in the title and responsibility statement fields.

  11. Application of Super-Synchronization Speed Control Technology in Two 80 MVA Motor-Generator Units of HL-2A

    International Nuclear Information System (INIS)

    Two sets of super-synchronization speed control assemblies for two 80 MVA motor-generator units have been developed successfully in order to satisfy the demand of the toroidal field system in the HL-2A tokamak. Based on the three-phase logical no-circumfluence a.c./a.c. cycloconverter, the speeds of two 2500 kW double fed drive motors have been regulated by means of the vector control technology. The maximum operating speed of each motor- generator unit has been raised from 1488 rpm (revolutions per minute) to 1650 rpm and the released energy of each unit during a pulsed discharge can reach 500 MJ. As a result, the toroidal field system has the capacity to provide 2.8 tesla (T) in HL-2A experiments

  12. Application of Super-Synchronization Speed Control Technology in Two 80 MVA Motor-Generator Units of HL-2A

    Science.gov (United States)

    Li, Huajun; Du, Chang; Xuan, Weiming; Pen, Jianfei; Hu, Haotian; Liu, Lin; Kang, Li; Xu, Lirong; Huang, Zhaorong; Wang, Fen; Wang, Xiaoping

    2007-04-01

    Two sets of super-synchronization speed control assemblies for two 80 MVA motor-generator units have been developed successfully in order to satisfy the demand of the toroidal field system in the HL-2A tokamak. Based on the three-phase logical no-circumfluence a.c./a.c. cycloconverter, the speeds of two 2500 kW double fed drive motors have been regulated by means of the vector control technology. The maximum operating speed of each motor- generator unit has been raised from 1488 rpm (revolutions per minute) to 1650 rpm and the released energy of each unit during a pulsed discharge can reach 500 MJ. As a result, the toroidal field system has the capacity to provide 2.8 tesla (T) in HL-2A experiments.

  13. Application of Super-Synchronization Speed Control Technology in Two 80 MVA Motor-Generator Units of HL-2A

    Institute of Scientific and Technical Information of China (English)

    LI Huajun; WANG Fen; WANG Xiaoping; DU Chang; XUAN Weiming; PEN Jianfei; HU Haotian; LIU Lin; KANG Li; XU Lirong; HUANG Zhaorong

    2007-01-01

    Two sets of super-synchronization speed control assemblies for two 80 MVA motorgenerator units have been developed successfully in order to satisfy the demand of the toroidal field system in the HL-2A tokamak.Based on the three-phase logical no-circumfluence a.c./a.c.cycloconverter,the speeds of two 2500 kW double fed drive motors have been regulated by means of the vector control technology.The maximum operating speed of each motor- generator unit has been raised from 1488 rpm (revolutions per minute) to 1650 rpm and the released energy of each unit during a pulsed discharge can reach 500 MJ.As a result,the toroidal field system has the capacity to provide 2.8 tesla (T) in HL-2A experiments.

  14. Indoor radon concentrations and radon doses at three districts of Ankara, Turkey and raising public awareness on the issue

    International Nuclear Information System (INIS)

    Indoor radon concentrations at METU, CIGDEM and DOSTLAR in Ankara were measured using electrets. The statistical analysis of the data indicated a lognormal distribution of radon concentrations, with no significant difference between CIGDEM and DOSTLAR with geometric means of GM = 87.5 and 54.5 Bq m-3, respectively. Radon concentrations did not change seasonally at CIGDEM which contain modern buildings, but at the slum district DOSTLAR, with poor insulation of houses a seasonal variation was observed. Annual effective radon doses were estimated (0.4-8.4 mSv). Public awareness about indoor radon was raised. (author)

  15. Evaluation of the children on trial in the Ankara 1st children s High Criminal Court for peer abuse

    OpenAIRE

    ŞENGÜL, Hasan Atakan; CANTÜRK, Nergis; Cantürk, Gürol; Dağalp, Rukiye

    2012-01-01

    Aim: Children performing acts defined as criminal law are the children who are dragged into crime When the victim of the identified actions are children both the number of children who need protection and who are dragged into crime increases and the victim of both sides in the incident become children Material and Method: The case files and cartons of decisions of 188 events sentenced in the Ankara 1st Children rsquo;s High Criminal Court for 4 year period between 2007 2010 in which victims a...

  16. EVALUATION OF THE ENVIRONMENTAL CONSCIOUSNESS OF THE STUDENTS IN A MEDICAL FACULTY IN ANKARA

    Directory of Open Access Journals (Sweden)

    Songul VAIZOÐLU

    2005-08-01

    Full Text Available Objectives: The reseach is conducted to evaluate the environmental consciousness of the students in a medical faculty in Ankara. Materials and Methods: Of the 412 students, 342 (83.0% participated this cross-sectional study. Data were collected in August 2000 with ?Environmental Consciousness Research Form?, the questionnaire prepared by the researchers and analysed using EPI INFO 6.0, a statistical package programme. Results: Mean age of the study group was 23.71 and 54.7% were male. Most of the students (74.4% reported that they would go to school/work by bycle, bus or on foot, and 87.1 percent of them told they would prefer train. Of the participants, 72.3% think environmental problems are not the issues only governments would care about. While most of them (81.8% reported that they would give up buying the products harmful to the environment, only 18.8% told they would want to deliver brochures about environment to each house. Nearly half of the students reported that they wouldn?t spend their money for the solution of environmental problems. Most of the students reported that they didn?t admit to any official institution to get information on environmental pollution (90.3%, never attended a meeting on environment (85.6% and never subscribed to a publication about the environment (91.4%. The students knew the products containing mercury, the substances which do not dissepear in the ocean and about the ozone layer depletion. Environmental consciousness scores are calculated by grading the questions about environmental consciousness. Mean score was 27.47. Nearly half of the students (53.2% had a score over 28. Students reported that deforestation (10.1%, air pollution (8.0%, population explosion (7.8%, nuclear weapons (7.3% and industrial wastes (7.1% would be the most important problems in 21. century. Conclusion: Complementing the lack of knowledge determined in the study by planning educational programmes, considering the environmental

  17. BURNOUT STATUS OF EMPLOYEES WORKING AT ANKARA FACTORIES AND SECOND REGIONAL DIRECTORATE OF TURKISH REPUBLIC STATE RAILWAY

    Directory of Open Access Journals (Sweden)

    Isil Irem BUDAKOÐLU

    2006-10-01

    Full Text Available Burnout is a person’s separation from the original mean of and the aim of his/her job. In this study we aimed to determine the burnout status of employees who were working at the factories and regional directorate of State Railways in Ankara. The study was applied at the wagon-locomotive care and repair, Ankara railway factories and 2nd Regional Directorate with an enrollment of 560 workers, on the date of March 2005. Maslach Burnout Inventory was used in order to determine the burnout status of workers. Emotional exhaustion scores of women, 39 years and younger workers, college or higher school graduates, white collar workers and chiefs are statistically higher than their counterparts (p<0.05. Workers who were younger than 39 years and were using alcohol had a statistically higher depersonalization scores than 40 years and older ones, and non alcohol users (p<0.05. However, the personal accomplishment score of high school and lower school graduates was statistically lower than college or higher school graduates (p<0.05. In conclusion, burnout status influences from demographic characteristics of workers, occupational group, title, monthly income and personal risk factors. [TAF Prev Med Bull 2006; 5(5.000: 317-325

  18. Safety and Immunogenicity of Boosting BCG Vaccinated Subjects with BCG: Comparison with Boosting with a New TB Vaccine, MVA85A

    OpenAIRE

    Whelan, KT; Pathan, AA; Sander, CR; Fletcher, HA; Poulton, I; Alder, NC; Hill, AV; Mcshane, H.

    2009-01-01

    OBJECTIVES To investigate the safety and immunogenicity of a booster BCG vaccination delivered intradermally in healthy, BCG vaccinated subjects and to compare with a previous clinical trial where BCG vaccinated subjects were boosted with a new TB vaccine, MVA85A. DESIGN Phase I open label observational trial, in the UK. Healthy, HIV-negative, BCG vaccinated adults were recruited and vaccinated with BCG. The primary outcome was safety; the secondary outcome was cellular immune responses ...

  19. Comportamento da parasitemia avaliada pelo método de Strout modificado em chagásicos agudos em tratamento

    Directory of Open Access Journals (Sweden)

    Cleudson Castro

    1988-12-01

    Full Text Available Em 18 pacientes com doença de Chagas aguda foi semiquantificada a parasitemia, pelo método de Strout modificado, antes e durante o tratamento. Antes da terapêutica a parasitemia variou entre 1 e 104 tripanossomos, e após o início do tratamento a parasitemia foi lida repetidamente com um intervalo, na maioria dos casos, entre dois e cinco dias, até a negativação. A dose inicial dos medicamentos foi de 10 a 15mg/kg/dia de Nifurtimox para sete pacientes, e 10 a 20mg/kg/dia de Benzonidazol para onze indivíduos. Após início do tratamento com Nifurtimox um paciente ficou o mínimo de cinco e dois o máximo de 23 dias com parasitemia enquanto com o Benzonidazol um paciente permaneceu o máximo de 15 dias.com parasitemia patente. O Benzonidazol baixou a parasitemia mais rapidamente que o Nifurtimox

  20. Estudo de propriedades de PVC modificado com grupos alquila e benzila Study of the properties of PVC modified through substitution by alkyl and benzyl groups

    Directory of Open Access Journals (Sweden)

    G. M Vinhas

    2005-07-01

    Full Text Available O poli(cloreto de vinila (PVC é o segundo termoplástico mais consumido em todo o mundo, sendo considerado o mais versátil dentre os plásticos. A grande versatilidade do PVC deve-se a suas propriedades e também a sua adequação aos mais variados processos de transformação. Uma vez que a resina de PVC é atóxica e inerte, a escolha de aditivos com essas mesmas características, permite a fabricação de filmes para embalagens de alimentos e produtos médico-hospitalares. Entretanto, suas propriedades podem se alterar quando submetido a qualquer processo de esterilização, especialmente por radiação gama. Neste trabalho foi realizado um estudo sobre a flexibilidade e estabilidade frente à radiação gama do PVC modificado quimicamente. O PVC foi modificado pela substituição de átomos de cloro por grupos alquila e benzila através de reações com reagentes de Grignard. Amostras de PVC modificado foram caracterizadas por espectroscopia de ressonância magnética nuclear de hidrogênio (RMN ¹H, espectroscopia na região do infravermelho com transformada de Fourier e calorimetria diferencial exploratória. A degradação do PVC modificado, quando submetido à radiação gama, foi avaliada por seus parâmetros viscosimétricos. De uma forma geral, o PVC modificado apresentou maior estabilidade à radiação gama que o original. O polímero modificado por substituição do cloro por grupo que contém um anel aromático foi o que apresentou maior estabilidade, quando submetido à dose de esterilização de 25 kGy. O PVC modificado apresentou temperaturas de transição vítrea ligeiramente mais baixas que o PVC original, indicando uma tendência ao aumento de flexibilidade pela presença de grupos alquila e benzila na cadeia do polímero.Poly(vinyl chloride (PVC ranks second among the most consumed thermoplastics in the world, and is considered the most versatile plastic. This versatility arises from its properties and suitability to a

  1. Study on Matching a 300 MVA Motor Generator with an Ohmic Heating Power Supply in HL-2M

    Science.gov (United States)

    Peng, Jianfei; Xuan, Weimin; Wang, Haibing; Li, Huajun; Wang, Yingqiao; Wang, Shujin

    2013-03-01

    A new 300 MVA/1350 MJ motor generator (MG) will be built to feed all of the poloidal field power supplies (PFPS) and auxiliary heating power supplies of the HL-2M tokamak. The MG has a vertical-shaft salient pole 6-phase synchronous generator and a coaxial 8500 kW induction motor. The Ohmic heating power supply (OHPS) consisting of 4-quadrant DC pulsed convertor is the one with the highest parameters among the PFPS. Therefore, the match between the generator and the OHPS is very important. The matching study with Matlab/Simulink is described in this paper. The simulation results show that the subtransient reactance of the generator is closely related to the inversion operation of the OHPS. By setting various subtransient reactance in the simulation generator model and considering the cost reduction, the optimized parameters are obtained as x″d = 0.405 p.u. at 100 Hz for the generator. The models built in the simulation can be used as an important tool for studying the dynamic characteristics and the control strategy of other HL-2M PFPSes.

  2. Study on Matching a 300 MVA Motor Generator with an Ohmic Heating Power Supply in HL-2M

    International Nuclear Information System (INIS)

    A new 300 MVA/1350 MJ motor generator (MG) will be built to feed all of the poloidal field power supplies (PFPS) and auxiliary heating power supplies of the HL-2M tokamak. The MG has a vertical-shaft salient pole 6-phase synchronous generator and a coaxial 8500 kW induction motor. The Ohmic heating power supply (OHPS) consisting of 4-quadrant DC pulsed convertor is the one with the highest parameters among the PFPS. Therefore, the match between the generator and the OHPS is very important. The matching study with Matlab/Simulink is described in this paper. The simulation results show that the subtransient reactance of the generator is closely related to the inversion operation of the OHPS. By setting various subtransient reactance in the simulation generator model and considering the cost reduction, the optimized parameters are obtained as x″d = 0.405 p.u. at 100 Hz for the generator. The models built in the simulation can be used as an important tool for studying the dynamic characteristics and the control strategy of other HL-2M PFPSes.

  3. Modeling of Electric Field Around 100 MVA 150/20 kV Power Transformator using Charge Simulation Method

    Directory of Open Access Journals (Sweden)

    Noviadi Arief Rachman

    2013-07-01

    Full Text Available Charge Simulation Method is one of the field theory that can be used as an approach to calculate the electromagnetic distribution on the electrical conductor. This paper discussed electric field modeling around power transformator by using Matlab to find the safety distance. The safe distance threshold of the electric field to human health refers to WHO and SNI was 5 kV/m. The specification of the power transformator was three phases, 150/20 kV, and 100 MVA. The basic concept is to change the distribution charge on the conductor or dielectric polarization charge with a set of discrete fictitious charge. The value of discrete fictitious charge was equivalent to the potential value of the conductor, and became a reference to calculate the electric field around the surface contour of the selected power transformator. The measurement distance was 5 meter on each side of the transformator surface. The results showed that the magnitude of the electric field at the front side was 5541 V/m, exceeding the safety limits.

  4. [Brucellosis, tularemia and borreliosis isolated from wild animals captured in Ankara, Konya, Urfa and Nevsehir provinces in Turkey].

    Science.gov (United States)

    Ozsan, K; Fazh, A; Aktan, M; Beyoğlu, K

    1976-10-01

    621 citellus, 41 Mus musculus, 35 microtus, 442 meriones, 70 Rattus rattus, 56 turtle, 89 hare, 1 hamster, 1 hedgehog and 1 sea snake, altogether 1379 wild animals were captured in Ankara, Konya, Urfa and Nevşehir. Neither Brucella or Francisella tularansis could be isolated nor borrelia could be seen. 1/40-1/80 agglutination titers obtained in 3 out of 134 sera taken from citellus, in 3 out of 264 sera taken from guinea pigs which were inoculated with spleen, liver and kidney suspensions of wild animals. 1/40-1/80 agglutination titers obtained against brucella antigen in 3 out of 125 sera taken from citellus. No significant agglutination titers could be obtained in 35 sera taken from guinea pigs which were inoculated with the organ suspensions of wild animals. In blood samples of 2 citellus few trypanosoma were detected. PMID:979704

  5. Jurassic–Paleogene intraoceanic magmatic evolution of the Ankara Mélange, north-central Anatolia, Turkey

    OpenAIRE

    E. Sarifakioglu; Dilek, Y.; M. Sevin

    2014-01-01

    Oceanic rocks in the Ankara Mélange along the Izmir–Ankara–Erzincan suture zone (IAESZ) in north-central Anatolia include locally coherent ophiolite complexes (~ 179 Ma and ~ 80 Ma), seamount or oceanic plateau volcanic units with pelagic and reefal limestones (96.6 ± 1.8 Ma), metamorphic rocks with ages of 256.9 ± 8.0 Ma, 187.4 ± 3.7 Ma, 158.4 ± 4.2 Ma, and 83.5 ± 1.2 Ma indicating northern Tethys during the late Paleozoic through Cretaceous, and subalkaline to alkaline volcanic and plutonic...

  6. REMOCIÓN DE CROMO VI DE SOLUCIONES ACUOSAS POR ADSORCIÓN SOBRE CARBONES ACTIVADOS MODIFICADOS

    Directory of Open Access Journals (Sweden)

    Nubia Céspedes

    2009-05-01

    Full Text Available En este trabajo se determina la adsorción de Cr6+ de disoluciones acuosas en dos carbones activados comerciales en función del grado de modificación (reducción, oxidación de la superficie de los adsorbentes con tratamientos térmicos en presencia de hidrógeno, oxígeno y ácido nítrico. La química superficial de los carbones activados originales y modificados se examina por los métodos de titulaciones de Böehm y descomposición con temperatura programada (DTP. Los ensayos de evaluación de la capacidad de adsorción se realizan con soluciones modelo de ión cromato y bajo distintas condiciones de concentración inicial, tiempo de contacto, pH del medio, temperatura y masa de adsorbente. Se comprueba que la modificación de la superficie de los adsorbentes afecta el grado de adsorción del ión cromato en la medida en que los sólidos tratados con H2 muestran la mayor  capacidad de remoción. Las variables que afectan favorablemente la extensión de la remoción de cromo son: concentración inicial de 100 ppm,  pH  inicial de  2,0, temperatura de 25 °C y  masa de adsorbente de 100 mg. 

  7. Teores de ácidos graxos em ovos comerciais convencionais e modificados com ômega-3 Fatty acids levels in conventional and designer omega-3 commercial eggs

    Directory of Open Access Journals (Sweden)

    Thaiz Marinho Magalhães Cedro

    2010-08-01

    Full Text Available Objetivou-se comparar os teores de ácidos graxos em ovos comerciais convencionais e em ovos modificados com ômega-3. Foram utilizadas 864 poedeiras da linhagem Isa Brown aleatoriamente distribuídas em dois grupos, de modo que cada gaiola com quatro aves foi considerada uma repetição, totalizando 216 repetições. No grupo para produção de ovos convencionais, as aves foram alimentadas durante toda a vida produtiva com ração à base de milho e farelo de soja, enquanto, no grupo para produção de ovos modificados, foram alimentadas, a partir da 22ª semana de idade, com ração contendo 1,5% de substrato de algas marinhas e 1,8% de óleo de peixe. Foram coletados, aleatoriamente, 180 ovos de cada grupo de poedeira (totalizando 360 ovos, que foram distribuídos em delineamento de blocos ao acaso, considerando cada ovo uma repetição. Os ovos modificados com ômega-3 apresentaram menores de índice de gema, mas não diferiram dos ovos convencionais quanto aos demais parâmetros de qualidade. As médias de ácidos graxos poliinsaturados ômega-3 e de ácidos graxos monoinsaturados foram mais altas nos ovos modificados. As relações entre ácidos graxos poliinsaturados e saturados e entre ômega-6 e ômega-3 nos ovos modificados foram próximas ao ideal estimado para alimentação humana. As médias de ácidos graxos poliinsaturados da série ômega-6 e de ácidos graxos saturados nos ovos convencionais foram significativamente maiores que dos ovos modificados com ômega-3.The objective of this work was to compare the levels of fatty acids between conventional commercial eggs and designer omega-3 eggs. It was used 864 Isa Brown line laying hens randomly distributed in two groups, so each cage with four birds was considered a replicate, totalizing 216 repetitions. In the group for production of conventional eggs, birds were fed corn- and soybean-based rations during the entire productive life while in the group for production of designer

  8. El Pennhip modificado y la sinfisiodesis juvenil pubiana como prevención de displasia de cadera canina en las Fuerzas Armadas

    OpenAIRE

    C. Sánchez Alonso; A. García Luque; M.J. Chamorro Sancho; P. Arias Sanz; P. De Vega Terán; F. Crespo Castejón

    2015-01-01

    Introducción: La displasia de cadera canina es la principal causa física de exclusión de los perros de los programas de adiestramiento en las Fuerzas Armadas y los Cuerpos y Fuerzas de Seguridad del Estado, es por ello importante realizar una criba temprana de esta patología. Objetivo: Determinar la eficacia del procedimiento del Penn-Hip modificado y la sinfisiodesis juvenil pubiana como método para prevenir el desarrollo de la displasia de cadera canina. Material y método: Estudio descripti...

  9. Efecto del recubrimiento a base de almidón de yuca modificado sobre la maduración del tomate

    OpenAIRE

    Paola Liceth Barco Hernández; Andrea Catalina Burbano Delgado; Silvio Andrés Mosquera Sánchez; Héctor Samuel Villada Castillo; Diana Paola Navia Porras

    2011-01-01

    Introducción. El tomate es un producto perecedero que requiere tratamientos para prolongar la vida útil, como por ejemplo el uso de recubrimientos. Objetivo. Se evaluó el efecto de la aplicación de un recubrimiento comestible a base de almidón modificado de yuca (Manihot esculenta Crantz) sobre el tomate (Solanum lycopersicum) larga vida bajo condiciones ambientales. Materiales y métodos. Se empleó un diseño completamente al azar para la valoración de los tratamientos, con tres réplicas y tre...

  10. Evaluación de la carbonatación de concreto modificado con polímero, utilizando agregado grueso y tereftalato de polietileno reciclados

    OpenAIRE

    Mendivil Escalante, José M.; Gómez Soberón, José Manuel Vicente; Castro Beltrán, Andrés; Corral Higuera, Ramón; Arredondo Rea, Susana Paola; Almaral Sánchez, Jorge Luis

    2015-01-01

    En este trabajo de investigación se han estudiado diferentes mezclas de concreto modificado con polímero, constituidas por agregado natural (AN) y un remplazo parcial de agregado grueso de concreto reciclado (AGR) del 25 %, con la adición de 9, 13 y 17% de resina polimérica sintetizada a partir de botellas de tereftalato de polietileno (PET por sus siglas en inglés) post-consumo a través de procesos de reciclaje mecánico y químico. Las probetas de concreto fabricadas fueron expuestas en una c...

  11. Análisis de un asfalto modificado con icopor y su incidencia en una mezcla asfáltica densa en caliente

    OpenAIRE

    Figueroa Infante, Ana Sofía; Reyes Lizcano, Fredy Alberto; Hernández Barrera, Diana; Jiménez, Christian; Bohórquez, Natalia

    2010-01-01

    En esta investigación se presentan los resultados obtenidos al elaborar una mezcla asfáltica MDC-2 con asfalto modificado con Icopor (poliestireno), resultado de la trituración de vasos desechables, con el ánimo de mejorar las propiedades mecánicas de las mezclas tipo rodadura y garantizar la durabilidad de las mismas bajo las solicitaciones impuestas en el período de diseño. Para el diseño se realizó la caracterización de los materiales pétreos, pertenecientes a una de las canteras de mayor ...

  12. Cultivo com recurso a organismos geneticamente modificados no Brasil e na União Europeia – uma análise comparativa

    OpenAIRE

    Fonseca, Ricardo; Aguiar, Ana; Sottomayor, Miguel

    2013-01-01

    Geneticamente Modificados (OGM) cresceu 6% atingindo cerca de 170 milhões de hectares, seguindo uma tendência sustentada de crescimento, que se verifica desde 1996 (ano da introdução da primeira cultura OGM). Commodities agrícolas fundamentais, como a soja, o milho, o algodão e a colza são crescentemente produzidas a partir de OGM. Hoje em dia, mais de 4/5 da soja globalmente produzida tem origem em OGM; no caso do milho, esta fracção já ultrapassou 1/3 da produção mundial. A r...

  13. El principio precautorio y el caso de los organismos genéticamente modificados en Argentina El principio precautorio y el caso de los organismos genéticamente modificados en Argentina

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    Silvina Gernaert Willmar

    2003-01-01

    Full Text Available Since it inception, at the Rio de Janeiro summit in 1992, the precautionary principle has evolved in terms of its interpretation and application. Both have made some progress in relation to genetically-modified organisms (GMO and its consequences for the domestic and international markets in Argentina. As regards the normative framework, Argentina does not dispose yet of well-established rules as to how the principle should be applied. This opens up the debate about the political standing of the principle. As for as international trade is concerned, Argentina is an important GMO producer but, when incertitude is the key element regarding the application of precaution, crucial discrepancies about the possibility of transforming the principle in to a political tool, rather than in to a legal tool emerge. In the case of the municipal ordinance on compulsory identification of genetically-modified food, the lack of experience about conditions of application of the precautionary principle might have led to justify it on the grounds of European directives.Desde su esbozo en la Declaración de Río de Janeiro en 1992, el principio precautorio ha ido evolucionando en cuanto a su interpretación y aplicación. En la Argentina, ambos han progresada con relación al tema de los Organismos Genéticamente Modificados (OGM y de sus implicaciones para el comercio interno internacional. En cuanta al marco normativo argentino, no existe aún una práctica clara que especifique las condiciones de aplicación del principio y esta carencia abre la discusión sobre su relevancia política. En relación can el comercio exterior la Argentina es un productor importante de productos derivados de los OGM, y, cuando la incertidumbre prima como rasgo del principio, surgen discrepancias relativas a la posibilidad de que la aplicación de la precaución pueda transformarse básicamente en una herramienta política más que en un instrumento de regulación legal. En el estudio

  14. Ankara Üniversitesi Uzaktan Eğitim Programına Katılan Öğrencilerin Akademik Başarılarını Yordayan Faktörler

    OpenAIRE

    Selma Şenel; Ömer Kutlu

    2015-01-01

    The aim of this study was to determine the factors predicting academic achievement of Ankara University Distance Education students. The study group of the research consists of 302 first grade students from Ankara University Distance Education Center (ANKUZEM) 2010-2011 associate degree programs. Data were formed in four groups respectively “individual characteristics”, “characteristics related with family and working environment”, “characteristics related with usage of informatio...

  15. [Tuberculosis Laboratory Surveillance Network (TuLSA) study group. The first step for national tuberculosis laboratory surveillance: Ankara, 2011].

    Science.gov (United States)

    Sezen, Figen; Albayrak, Nurhan; Özkara, Şeref; Karagöz, Alper; Alp, Alpaslan; Duyar Ağca, Filiz; İnan Süer, Asiye; Müderris, Tuba; Ceyhan, İsmail; Durmaz, Rıza; Ertek, Mustafa

    2015-04-01

    The most effective method for monitoring country-level drug resistance frequency and to implement the necessary control measures is the establishment of a laboratory-based surveillance system. The aim of this study was to summarize the follow up trend of the drug-resistant tuberculosis (TB) cases, determine the load of resistance and evaluate the capacities of laboratories depending on laboratory quality assurance system for the installation work of National Tuberculosis Laboratory Surveillance Network (TuLSA) which has started in Ankara in 2011. TuLSA studies was carried out under the coordination of National Tuberculosis Reference Laboratory (NRL) with the participation of TB laboratories and dispensaries. Specimens of TB patients, reported from health institutions, were followed in TB laboratories, and the epidemiological information was collected from the dispensaries. One isolate per patient with the drug susceptibility test (DST) results were sent to NRL from TB laboratories and in NRL the isolates were rechecked with the genotypical (MTBDRplus, Hain Lifescience, Germany) and phenotypical (MGIT 960, BD, USA) DST methods. Molecular epidemiological analysis were also performed by spoligotyping and MIRU/VNTR. Second-line DST was applied to the isolates resistant to rifampin. A total of 1276 patients were reported between January 1st to December 31th 2011, and 335 cases were defined as "pulmonary TB from Ankara province". The mean age of those patients was 43.4 ± 20 years, and 67.5% were male. Three hundred seventeen (94.6%) patients were identified as new cases. The average sample number obtained from pulmonary TB cases was 3.26 ± 2.88, and 229 (68.3%) of them was culture positive. DST was applied to all culture positive isolates; 90.4% (207/229) of cases were susceptible to the five drugs tested (ethambutol, isoniazid, pyrazinamide, rifampicin, streptomycin). Eight (3.5%) of the isolates were multidrug-resistant (MDR-TB), while no extensively drug

  16. A Phase I Randomized Therapeutic MVA-B Vaccination Improves the Magnitude and Quality of the T Cell Immune Responses in HIV-1-Infected Subjects on HAART

    OpenAIRE

    Gómez, Carmen E.; Perdiguero, Beatriz; García Arriaza, Juan; Cepeda, Victoria; Sánchez-Sorzano, Carlos Óscar; Mothe, B; Jiménez, José Luis; Muñoz Fernández, María Ángeles; Gatell, J. M.; López Bernaldo de Quirós, Juan Carlos; Brander, Christian; Garcia, Felipe; Esteban, Mariano

    2015-01-01

    Trial Design Previous studies suggested that poxvirus-based vaccines might be instrumental in the therapeutic HIV field. A phase I clinical trial was conducted in HIV-1-infected patients on highly active antiretroviral therapy (HAART), with CD4 T cell counts above 450 cells/mm3 and undetectable viremia. Thirty participants were randomized (2:1) to receive either 3 intramuscular injections of MVA-B vaccine (coding for clade B HIV-1 Env, Gag, Pol and Nef antigens) or placebo, followed by interr...

  17. A phase I randomized clinical trial of candidate human immunodeficiency virus type 1 vaccine MVA.HIVA administered to Gambian infants.

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    Muhammed O Afolabi

    Full Text Available BACKGROUND: A vaccine to decrease transmission of human immunodeficiency virus type 1 (HIV-1 during breast-feeding would complement efforts to eliminate infant HIV-1 infection by antiretroviral therapy. Relative to adults, infants have distinct immune development, potentially high-risk of transmission when exposed to HIV-1 and rapid progression to AIDS when infected. To date, there have been only three published HIV-1 vaccine trials in infants. TRIAL DESIGN: We conducted a randomized phase I clinical trial PedVacc 001 assessing the feasibility, safety and immunogenicity of a single dose of candidate vaccine MVA.HIVA administered intramuscularly to 20-week-old infants born to HIV-1-negative mothers in The Gambia. METHODS: Infants were followed to 9 months of age with assessment of safety, immunogenicity and interference with Expanded Program on Immunization (EPI vaccines. The trial is the first stage of developing more complex prime-boost vaccination strategies against breast milk transmission of HIV-1. RESULTS: From March to October 2010, 48 infants (24 vaccine and 24 no-treatment were enrolled with 100% retention. The MVA.HIVA vaccine was safe with no difference in adverse events between vaccinees and untreated infants. Two vaccine recipients (9% and no controls had positive ex vivo interferon-γ ELISPOT assay responses. Antibody levels elicited to the EPI vaccines, which included diphtheria, tetanus, whole-cell pertussis, hepatitis B virus, Haemophilus influenzae type b and oral poliovirus, reached protective levels for the vast majority and were similar between the two arms. CONCLUSIONS: A single low-dose of MVA.HIVA administered to 20-week-old infants in The Gambia was found to be safe and without interference with the induction of protective antibody levels by EPI vaccines, but did not alone induce sufficient HIV-1-specific responses. These data support the use of MVA carrying other transgenes as a boosting vector within more complex prime

  18. Delivery of antigenic candidates by a DNA/MVA heterologous approach elicits effector CD8+T cell mediated immunity against Trypanosoma cruzi

    OpenAIRE

    Gupta, Shivali; Garg, Nisha Jain

    2012-01-01

    In this study, we have characterized the immune mechanisms elicited by antigenic candidates, TcG2 and TcG4, delivered by a DNA-prime/MVA-boost approach, and evaluated the host responses to T. cruzi infection in C57BL/6 mice. Immunization of mice with antigenic candidates elicited antigen-specific, high-avidity, trypanolytic antibody response (IgG2b>IgG1) and CD8+T cells that exhibited type-1 cytolytic effector (CD8+CD107a+IFN-γ+Perforin+) phenotype. The extent of TcG2-dependent type 1 B and T...

  19. Perfil de rendimiento en el campeonato del mundo de baloncesto (Ankara 2013) para personas con discapacidad intelectual: implicaciones en el sistema de elegibilidad

    OpenAIRE

    Pérez Tejero, Javier; Pinilla Arbex, Javier; Vanlandewijck, Yves

    2015-01-01

    [ES] Este estudio tiene por objetivo caracterizar el perfil de rendimiento individual del jugador de baloncesto con DI y distinguir entre los equipos ganadores y perdedores. Para ello, en línea con la literatura científica en el baloncesto convencional, se analizaron las estadísticas oficiales de juego del Campeonato del Mundo de baloncesto para jugadores con DI (Ankara, 2013).

  20. The Use of Alternate Ligno-cellulosic Raw Materials Banana (Musa sapientum) Ankara (Calotropis procera ) and Pineapple (Ananas comosus ) in Handmade Paper & their Blending with Waste Paper.

    OpenAIRE

    Atul Kumar

    2013-01-01

    The studies were made to established suitability of lingo-cellulosic raw materials namely leaf fibreBanana (Musa Sapientum), bast fibre Ankara (Calotropis Procera), & leaf fibre Pineapple (Ananas Comosus) for making pulps for handmade paper industry. This should help in providing a cost effective, good quality cellulosic raw material as an alternate to cost prohibitive traditionally used cotton hosiery waste traditionally used for manufacturing good quality handmade paper & it’s products. Th...

  1. The Effects of Sociotropic- Autonomic Personality Characteristics on the Motivation of the Workers: A Study Conducted In Accommodation Facilities in Ankara

    OpenAIRE

    ÖKTEM, Şükran; TURGUT, Hakan; TOKMAK, İsmail

    2013-01-01

    Because of the consideration that staff personality characteristics effect their motivation, staff of four and five star hotels, located within the city borders of Ankara, have been given priority while determining their sociotropic-autonomic personality traits. Subsequently, motivations of the said staff have been measured in accordance with both inner and external motivation tools in order to determine whether there is an effect of sociotropical-autonomic personality traits on inner and...

  2. Determination of pathogens causing damping-off and their pathogenicity in tomato seedbeds in Ankara (Ayaş, Beypazarı and Nallıhan districts) province

    OpenAIRE

    AŞKIN, Ayşe; KATIRCIOĞLU, Y. Zekai

    2009-01-01

    This research was carried out for determination of pathogens causing damping-off and their pathogenicity in tomato seedbeds in Ankara province (Ayaş, Beypazarı and Nallıhan districts) in 2003. Two hundred eleven samples of tomato seedlings, thought to be infected with damping off were collected from 42 seedbeds. Fusarium spp., Pythium spp., Rhizoctonia solani, Alternaria spp. and Aspergillus spp. were isolated from all collected  tomato seddlings. Fusarium spp., Pythium spp., R. solani a...

  3. Escore US7 modificado na avaliação de sinovite em pacientes com artrite reumatoide inicial

    Directory of Open Access Journals (Sweden)

    José Alexandre Mendonça

    2014-07-01

    Full Text Available Objetivo: Avaliar o escore US7 modificado (escore MUS7 SIN na avaliação de pacientes com artrite reumatoide inicial (ARI. Além disso, foram examinados recessos dorsais e palmares dos punhos, bem como pequenas articulações das mãos e dos pés, para o diagnóstico de sinovite, mediante uma avaliação global das articulações. Métodos: A amostra do estudo compreendeu 32 pacientes tratados para artrite, com 13 meses como duração média da doença. Foi utilizado um aparelho de ultrassonografia (US com transdutor de alta frequência. As mãos dos participantes também foram radiografadas e analisadas pelo escore de Larsen. Resultados: Nas 832 articulações examinadas, detectou-se sinovite em 173 (20,79%, tenossinovite em 22 (4,91% e erosões em três (1,56%. A sinovite foi predominantemente detectada no recesso dorsal (73,38% das articulações MCF e IFP, quando comparado com o recesso palmar (26%. A presença de sinovite nas articulações avaliadas teve correlação com os resultados clínicos (HAQ-DI, DAS28, laboratoriais (anti-PCC, FR, PCR e ultrassonográficos (r = 0,37 a r = 0,42; p = 0,04 a p = 0,003. Encontramos correlação do escore MUS7 SIN para US na técnica da escala de cinzas (gray scale ou na técnica de Doppler de amplitude (power Doppler com os valores do instrumento DAS28 (PCR (r = 0,38; p = 0,0332 e com os resultados da PCR (r = 0,39; p = 0,0280, respectivamente. Conclusão: O recesso dorsal, o punho e as pequenas articulações podem ser considerados como locais importantes para a detecção de sinovite pelo escore MUS7 SIN em pacientes com ARI.

  4. Deletion of A44L, A46R and C12L Vaccinia Virus Genes from the MVA Genome Improved the Vector Immunogenicity by Modifying the Innate Immune Response Generating Enhanced and Optimized Specific T-Cell Responses

    Science.gov (United States)

    Holgado, María Pía; Falivene, Juliana; Maeto, Cynthia; Amigo, Micaela; Pascutti, María Fernanda; Vecchione, María Belén; Bruttomesso, Andrea; Calamante, Gabriela; del Médico-Zajac, María Paula; Gherardi, María Magdalena

    2016-01-01

    MVA is an attenuated vector that still retains immunomodulatory genes. We have previously reported its optimization after deleting the C12L gene, coding for the IL-18 binding-protein. Here, we analyzed the immunogenicity of MVA vectors harboring the simultaneous deletion of A44L, related to steroid synthesis and A46R, a TLR-signaling inhibitor (MVAΔA44L-A46R); or also including a deletion of C12L (MVAΔC12L/ΔA44L-A46R). The absence of biological activities of the deleted genes in the MVA vectors was demonstrated. Adaptive T-cell responses against VACV epitopes, evaluated in spleen and draining lymph-nodes of C57Bl/6 mice at acute/memory phases, were of higher magnitude in those animals that received deleted MVAs compared to MVAwt. MVAΔC12L/ΔA44L-A46R generated cellular specific memory responses of higher quality characterized by bifunctionality (CD107a/b+/IFN-γ+) and proliferation capacity. Deletion of selected genes from MVA generated innate immune responses with higher levels of determining cytokines related to T-cell response generation, such as IL-12, IFN-γ, as well as IL-1β and IFN-β. This study describes for the first time that simultaneous deletion of the A44L, A46R and C12L genes from MVA improved its immunogenicity by enhancing the host adaptive and innate immune responses, suggesting that this approach comprises an appropriate strategy to increase the MVA vaccine potential. PMID:27223301

  5. Mineralogical, geochemical and micromorphological evaluation of the Plio-Quaternary paleosols and calcretes from Karahamzall, Ankara (Central Turkey

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    Küçükuysal Ceren

    2014-06-01

    Full Text Available We present the mineralogical, micromorphological, and geochemical characteristics of the paleosols and their carbonates from Karahamzall, Ankara (Central Turkey. The paleosols include calcretes of powdery to nodular forms and alternate with channel deposits. The presence of pedofeatures, such as clay cutans, floating grains, circumgranular cracks, MnO linings, secondary carbonate rims, traces of past bioturbation and remnants of root fragments are all the evidence of pedogenesis. Bw is the most common soil horizon showing subangular-angular blocky to granular or prismatic microstructures. Calcretes, on the other hand, are evaluated as semi-mature massive, nodular, tubular or powdery forms. The probable faunal and floral passages may also imply the traces of life from when these alluvial deposits were soil. The presence of early diagenetic palygorskite and dolomite together with high salinization, high calcification and low chemical index of alteration values are evidence of the formation of calcretes under arid and dry conditions. δ13C compositions of the carbonates ranging from -7.11 ‰ to -7.74 ‰ VPDB are comformable with the world pedogenic carbonates favouring the C4 vegetation; likely δ18O compositions of the carbonates are between -3.97 ‰ and -4.91 ‰ which are compatible with the paleosols formed under the influence of meteroic water in the vadose zone

  6. Evaluation of Sun Protection Habits and Melanocytic Nevi of Population Screened in a Shopping Mall in Ankara

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    Banu Öztürk

    2009-12-01

    Full Text Available Background and Design: The evaluation of sun protection habits, numbers and types of nevi in our community. Material and Method: A stand was built in a big mall in Ankara where 764 patients were screened for the presence of nevi in three working day periods during the month of May of 2005 and 2006. Lesions were evaluated with the help of digital dermatoscopy. In the study, sun protective practices, sunscreen using habits, dermatoscopic diagnosis of the lesions, numbers and the localization of the lesions were also recorded. Results: The study population consisted of 764 patients (438 female, 326 male with age ranging from 1 to 80 (33.7±13.7 years. 55.6% of them had skin type III, 47.3% had sunburn history. Most of the patients (44.1% were not using sunscreens. Hats and umbrellas (40.3% were the most common accessories used for protection. 904 lesions of 764 patients examined by dermatoscopy revealed that the most common diagnosis was nevi. Two patients were diagnosed with having cutaneous melanoma or basal cell carcinoma. Conclusion: Our findings have shown that the general population is not aware of the risks of sun exposure or the appropriate ways for sun protection and that there is a necessity for educational programmes or campaigns about sun protection and nevi in our general population.

  7. Sensitivity analyses for the DTMs derived from Unmanned Aerial Vehicle (UAV) in gully erosion mapping: Nallihan badland area (Ankara, Turkey)

    Science.gov (United States)

    Avdan, Ugur; Gorum, Tolga; Comert, Resul; Nefeslioglu, Hakan

    2015-04-01

    The main purpose of this study is to evaluate the spatial resolutions for the Digital Terrain Models (DTMs) derived from Unmanned Aerial Vehicle (UAV) in gully erosion mapping. For the purpose, Nallihan badland area (Ankara, Turkey) was selected to be the experimental site. The investigations were carried out in 3 stages; (i) production of the DTMs having 3 cm and 9 cm spatial resolutions by using the orthophoto imagery acquired from the UAV at 97.5 m and 292.4 m altitudes, respectively, (ii) Terrestrial Laser Scanning (TLS) of the experimental site and production of the DTMs derived from the TLS data resampled at 3 cm and 9 cm spatial resolutions, and (iii) spatial and profile comparisons of the derived data. The average altitude differences were obtained on the intervals (-0.1, 0.1) m and (-0.2, 0.2) m for the comparisons between TLS-3cm and UAV-3cm, and TLS-9cm and UAV-9cm data, respectively. Additionally, considering the profile comparisons, it is revealed that depending on the decreasing of spatial resolution, the erosion rates calculated from the DTMs increase artificially.

  8. The Rates of Undergoing Cancer Screening Among Family Physicians Aged 40 and Older Working in Ankara Province

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    Ayşe Selda Tekiner

    2016-04-01

    Full Text Available Aim: Family physicians, as role models for their patients, self health behaviors are important. We aimed to investigate the rate of undergoing cancer screening among family physicians. Methods: This is a descriptive and questionnaire-based study. Although we aimed to interview 1100 family physicians aged 40 or older working at family health centers of Ankara, the study was performed with 453 physicians. The questionnaire form comprised of the doctors’ opinions about cancer screening tests, and the situations of undergoing screening tests. Results: The rates of mammography and Pap smear test were 60% and 59% respectively among female doctors. The rates of fecal occult blood testing and colonoscopy were 2.7% and 8.6% respectively. The physicians’ responses to the question “family physicians should be responsible for cancer screening tests in populations they serve” were: “I totally agree": 9.3%; “I agree ": 23.6%; “I am undecided": 21.6%; “I disagree": 36.9%; "I strongly disagree": 8.6% Conclusion: Family physicians who have the responsibility of public health should also undergo screening tests regularly. The importance of protecting own health should be stressed at both undergraduate and postgraduate training programs. Special permits granted by administrators for health checks may also be encouraging.

  9. Exposure of newborns to aflatoxin M1 and B1 from mothers' breast milk in Ankara, Turkey.

    Science.gov (United States)

    Gürbay, A; Sabuncuoğlu, S Atasayar; Girgin, G; Sahin, G; Yiğit, S; Yurdakök, M; Tekinalp, G

    2010-01-01

    Aflatoxins (AFs) are important risks for human health due to their widespread presence in foods and environment. However, contamination risk of breast milk with different pollutants including AFs is high in today's life conditions. Since breast milk is a major nutrient for infants, feeding of infants with safe milk is essential. Therefore, the objective of this study was to determine the levels of AF M(1) and B(1) in breast milk samples collected from 75 mothers in Ankara, Turkey. AF M(1) and B(1) levels were investigated by high performance liquid chromatography (HPLC) with a fluorescence detector following an extraction procedure. The limit of detection was found to be 5 ng/l. Both AFs were detected in diverse degrees in all breast milk samples: The level of AF M(1) were in the ranges of 60.90-299.99 ng/l, and AF B(1) were in the ranges of 94.50-4123.80 ng/l. These results pointed out the exposure of mothers and neonates to AF M(1) and B(1), and the necessity of further research on mycotoxin contamination both in foods and biological fluids as well as protection strategies. PMID:19850097

  10. Precaución, riesgo y sostenibilidad en los organismos agrícolas modificados genéticamente Precaución, riesgo y sostenibilidad en los organismos agrícolas modificados genéticamente

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    J. David Tábara

    2003-01-01

    Full Text Available El cultivo y la comercialización de Organismos Agrícolas Modificados Genéticamente (0MG es origen de multitud de importantes polémicas sociales, económicos y ecológicas, en cuya resolución a menudo se reclama la incorporación del principio y de prácticas de precaución. Este artículo introduce y analiza algunas de estas discusiones a partir de la perspectiva aportada por el proyecto europeo Precautionary Expertise for GM Crops (PEG sobre la aplicación de la precaución en el desarrollo y la comercialización de OMG agrícolas. A partir del material recogido en el caso español, los autores argumentan que gran parte de los desacuerdos que surgen de en la interpretación y aplicación de la precaución con relación a los OMG derivan de la adopción de una perspectiva más o menos reduccionista, o más o menos sistémica, tanto en la evaluación y regulación de los riesgos como en la bondad, los costes o los beneficios de su utilización. Por ello es posible detectar analíticamente, paro el caso español, una clara oposición entre aquellas interpretaciones de la precaución caso por caso, de aquellas que sostienen la necesidad de aplicar prácticas de evaluación y de gestión basadas en una precaución sistémica, más cercano a los principios y los postulados políticos de la sostenibilidad.The debate over GMO crops and their commercialisation becomes the source of a large number of conflicts with important social, economic and ecologic consequences the resolution of which often calls for the application of a precautionary approach and of the precautionary principle. This article introduces and analyses such discussions, and does so within the framework provided by the EU project Precautionary Expertise for GM (PEG. Based on first material gathered for the Spanish case study, the authors argue that a large majority of the disagreements around practical application of precaution over agricultural GMO in Spain come from a conception

  11. Publicidade: o corpo modificado

    Directory of Open Access Journals (Sweden)

    Tânia Márcia Cezar Hoff

    2008-09-01

    Full Text Available Neste artigo, resultado parcial de uma pesquisa a respeito do imaginário do corpo na publicidade brasileira, analisamos alguns aspectos da representação de corpo em anúncios de clínicas de estética e de cirurgia plástica e também em depoimentos veiculados em revistas do mercado editorial brasileiro em janeiro e fevereiro de 2004. Nos referidos anúncios, identificamos uma desqualificação do corpo "natural" e uma necessidade de modificá-lo por meio de cirurgias. Também identificamos que a publicidade, associando os discursos médico e tecnológico, propõe um novo paradigma de corpo. Palavras-chaves: corpo, cirurgia, publicidade. ABSTRACT In this article, as apartial result of a research on the imaginary body in the Brazilian advertisement, we analyzed some aspects of body representation in advertising for a esthetic and lastic surgery clinics and also in narratives published in some magazines from the Brazilian editorial market during January and February of 2004. In the referred group of advertising, we identified the existence of a negative qualification of "natural" body and a necessity of modijying it through surgery. We also identified that the advertisement, associating the medical and technological discourses, proposes a new body paradigm. Keywords: body, cirurgic intervention, advertising.

  12. GENETICALLY MODIFIED FOOD CROPS AND PUBLIC HEALTH Cultivos alimenticios genéticamente modificados y salud pública

    Directory of Open Access Journals (Sweden)

    ORLANDO ACOSTA

    salud pública y las economías tradicionales. A pesar de los avances en la agricultura de los cultivos alimenticios, la situación actual a nivel mundial está caracterizada por una hambruna masiva y por una desnutrición crónica, lo cual constituye un importante problema de salud pública. Los cultivos GM biofortificados se han considerado como una estrategia importante y complementaria para suministrar alimentos básicos naturalmente fortificados a las poblaciones con desnutrición. Las recomendaciones de los expertos y las preocupaciones públicas han conducido al diseño de estrategias para la evaluación de los riesgos potenciales de la producción y el consumo de los cultivos GM. El objetivo de la presente revisión crítica es la exposición de algunos puntos de vista en conflicto sobre los riesgos potenciales de los cultivos GM para la salud pública. Se concluye que los cultivos alimenticios GM no son más riesgosos que aquellos modificados genéticamente con los métodos convencionales, y que estos cultivos GM podrían contribuir a la reducción de la población con desnutrición en el mundo, pero se necesita que esto sea complementado con acciones políticas efectivas dirigidas a incrementar los ingresos de la población que vive por debajo de la línea de pobreza.

  13. Research of Stability Problems on Ankara-Konya High Speed Railway Line (Turkey) using Ground Penetrating Radar and Petrographical Methods

    Science.gov (United States)

    Kadioglu, S.; Kadioglu, Y. K.

    2012-04-01

    The aim of the study is to research the stability problems according to rock properties and their discontinuities such as fractures, faults and karstic cavities on the new high-speed railway line between the capital city Ankara and the largest city Konya in Turkey. The Ankara-Konya high speed railway including a tunnel managed from The Turkish State Railways (TCDD). Geological surveys, polarizing microscope and confocal Raman spectrometry studies were used to determine rock properties. Ground penetrating radar (GPR) method was used to determine faults, fractures and karstic cavities. The railway line has been mainly constructed on inner Tauride Ocean suture of the Central Anatolia. The basement unit of the railway line mainly has been composed of ophiolitic complex of the inner Tauride Ocean. The main lithology of this ophiolitic complex has been formed by radiolarite, pelagic sediments, dolarite, gabbro, serpentinized peridotite and limestone blocks. The Jurassic alloctonous limestone which has been thrust on the ophiolitic complex. Neogene cover young units with minor amount of Alluvium deposits have been formed by the upper litholgy in the region. The serpentinite and altered radiolarite formation are formed by lubricous ground for the railway line in the region. A RAMAC CUII GPR system was used with a bi-static 100 MHz center band shielded antenna to acquire profile data. Totaly 35 km was surveyed on different parts of the railway line by considering the results of the geologic research and petrograpical studies. When we started to study, rail construction of some parts of the line had already been completed. Therefore, during studies, we gathered the data on the backfilled way on the three parallel profiles spaced 1m apart or on the service way next to the railway line. There was a tunnel on the line. We also gathered two parallel profiles data on the tunnel and four profiles data next to the tunnel to evaluate the stability according to the discontinuities

  14. Jurassic–Paleogene intraoceanic magmatic evolution of the Ankara Mélange, north-central Anatolia, Turkey

    Directory of Open Access Journals (Sweden)

    E. Sarifakioglu

    2014-02-01

    Full Text Available Oceanic rocks in the Ankara Mélange along the Izmir–Ankara–Erzincan suture zone (IAESZ in north-central Anatolia include locally coherent ophiolite complexes (~ 179 Ma and ~ 80 Ma, seamount or oceanic plateau volcanic units with pelagic and reefal limestones (96.6 ± 1.8 Ma, metamorphic rocks with ages of 256.9 ± 8.0 Ma, 187.4 ± 3.7 Ma, 158.4 ± 4.2 Ma, and 83.5 ± 1.2 Ma indicating northern Tethys during the late Paleozoic through Cretaceous, and subalkaline to alkaline volcanic and plutonic rocks of an island arc origin (~ 67–63 Ma. All but the arc rocks occur in a shale–graywacke and/or serpentinite matrix, and are deformed by south-vergent thrust faults and folds that developed in the middle to late Eocene due to continental collisions in the region. Ophiolitic volcanic rocks have mid-ocean ridge (MORB and island arc tholeiite (IAT affinities showing moderate to significant large ion lithophile elements (LILE enrichment and depletion in Nb, Hf, Ti, Y and Yb, which indicate the influence of subduction-derived fluids in their melt evolution. Seamount/oceanic plateau basalts show ocean island basalt (OIB affinities. The arc-related volcanic rocks, lamprophyric dikes and syenodioritic plutons exhibit high-K shoshonitic to medium- to high-K calc-alkaline compositions with strong enrichment in LILE, rare earth elements (REE and Pb, and initial εNd values between +1.3 and +1.7. Subalkaline arc volcanic units occur in the northern part of the mélange, whereas the younger alkaline volcanic rocks and intrusions (lamprophyre dikes and syenodioritic plutons in the southern part. The late Permian, Early to Late Jurassic, and Late Cretaceous amphibole-epidote schist, epidote-actinolite, epidote-chlorite and epidote-glaucophane schists represent the metamorphic units formed in a subduction channel in the northern Neotethys. The Middle to Upper Triassic neritic limestones spatially associated with the

  15. Frequency, risk factors and preventive approach to fall among aged population living in a nursing home in Ankara

    Directory of Open Access Journals (Sweden)

    Evren Kibar

    2015-02-01

    Full Text Available AIM: Increase in aged population in number brings along the risk of falls and fall-related injuries among elderly. It has been reported that almost 60% of falls occur in nursing homes and majority of admissions to emergency departments due to falls consist of elderly. The purpose of this study conducted in a nursing home in Ankara was to determine the frequency of falls and risk factors, and to clear out the opinions and views of the participants on planning and promoting interventions for prevention. METHOD: This cross-sectional study was carried out among 60 years of age and older residents who lived in a nursing home. Data of the study was collected via face to face interviewing technique. The questionnaire consisted of four sections including socio-demographic characteristics, health status of the participants, healthy/risky behavior and fall related characteristics. RESULTS: Mean age of the 75 female and 59 male participants involved in the study was 73.99+/-7.18. Females were found to fall more in frequency than males (p>0.05. It was found that nearly half of the males (47.5% and more than half of the females (56.0% fell at least once within the previous year. Number of falls were higher among 75 years of age and older participants compared to the other age groups (p=0.003. Compared to the participants with fall background, aged people without fall background gave more correct answers in number to the questions which were asked to assess the knowledge on falls. Six out of 20 answers were statistically significantly correct (p <0.05. CONCLUSION: Individual and environmental interventions to be continued both inside and outside the institutions in order to prevent falls. [TAF Prev Med Bull 2015; 14(1.000: 23-32

  16. EFFECTS OF PERSONALITY TRAITS OF SPORTS-HEALTH CENTER EMPLOYEES ON SERVICE ORIENTATION IN PROVINCE OF ANKARA

    Directory of Open Access Journals (Sweden)

    Serdar

    2016-04-01

    Full Text Available This study was made for the purpose of researching the effects of personality traits of sports-health center personnel on service orientation in province of Ankara. As data collection tool, “Five Factor Personality Inventory” developed by Goldberg in 1990 and a version of “Customer Centricity Scale” developed by Brown et al. (2000, which was localized and tested for validity-reliability by Serçeoğlu (2013, were used. In the mentioned scale, the factor load values representing the structure validity of items constituting the service orientation change between 0,85 and 0,97. Cronbach Alpha reliability coefficient calculated for the reliability of the scale was calculated as 0,98 (Serçeoğlu, 2013. Data collected from 201 participants working at 19 businesses were analyzed by processing in SPSS-21 package software. The conclusion of personality traits of personnel working at sports-health centers having a significant effect on the service orientation, also personality traits sub-factors having a significant effect on service orientation was reached. When the effect of personality traits of sports-health business employees’ belonging to sub-factors of five factor personality inventory on service orientation is looked at; the sub-factors of agreeableness, responsibility and being open to new ideas are seen to have significant effect, the sub-factors of extroversion and emotional balance are seen not to have significant effect. The conclusion of significant difference between the personality traits and service orientation of the participants according to their status in the department they are employed was reached. It was identified that there were no significant difference between personality traits and service orientation according to gender, marital status and duration of working in the industry of the employees.

  17. MVA-based H5N1 vaccine affords cross-clade protection in mice against influenza A/H5N1 viruses at low doses and after single immunization.

    Directory of Open Access Journals (Sweden)

    Joost H C M Kreijtz

    Full Text Available Human infections with highly pathogenic avian influenza viruses of the H5N1 subtype, frequently reported since 2003, result in high morbidity and mortality. It is feared that these viruses become pandemic, therefore the development of safe and effective vaccines is desirable. MVA-based H5N1 vaccines already proved to be effective when two immunizations with high doses were used. Dose-sparing strategies would increase the number of people that can be vaccinated when the amount of vaccine preparations that can be produced is limited. Furthermore, protective immunity is induced ideally after a single immunization. Therefore the minimal requirements for induction of protective immunity with a MVA-based H5N1 vaccine were assessed in mice. To this end, mice were vaccinated once or twice with descending doses of a recombinant MVA expressing the HA gene of influenza virus A/Vietnam/1194/04. The protective efficacy was determined after challenge infection with the homologous clade 1 virus and a heterologous virus derived from clade 2.1, A/Indonesia/5/05 by assessing weight loss, virus replication and histopathological changes. It was concluded that MVA-based vaccines allowed significant dose-sparing and afford cross-clade protection, also after a single immunization, which are favorable properties for an H5N1 vaccine candidate.

  18. Combination of intratumoral injections of vaccinia virus MVA expressing GM-CSF and immunization with DNA vaccine prolongs the survival of mice bearing HPV16 induced tumors with downregulated expression of MHC class I molecules

    Czech Academy of Sciences Publication Activity Database

    Němečková, Š.; Šmahel, M.; Hainz, P.; Macková, J.; Zurková, K.; Gabriel, P.; Indrová, Marie; Kutinová, L.

    2007-01-01

    Roč. 54, č. 4 (2007), s. 326-333. ISSN 0028-2685 R&D Projects: GA MZd NR8004 Institutional research plan: CEZ:AV0Z50520514 Keywords : vaccinia virus MVA expressing GM-CSF * DNA vaccine * HPV16 induced tumors Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 1.208, year: 2007

  19. Síntesis de zeolita lta: optimización de las condiciones de síntesis, usando el método simplex secuencial modificado

    OpenAIRE

    García Soto, Andrés Ricardo; Rodríguez Niño, Gerardo; Trujillo, Carlos Alexander

    2012-01-01

    Este artículo presenta un estudio del efecto simultáneo de las variables de síntesis más significativas en la obtención de Zeolita LTA. Se aplicó el método simplex modificado para determinar las condiciones óptimas de síntesis, definiendo como función y objetivo la cristalinidad relativa de la zeolita obtenida y evaluada mediante la difracción de rayos X. Se requirieron 24 ensayos para que el método convergiera de manera óptima, logrando así, una cristalinidad de 95,4 %, obtenida para las rel...

  20. A falta de informação sobre os Organismos Geneticamente Modificados no Brasil The lack of information on Genetically Modified Organisms in Brazil

    OpenAIRE

    Isabelle Geoffroy Ribeiro; Victor Augustus Marin

    2012-01-01

    O presente artigo apresenta uma revisão sobre a rotulagem de produtos que apresentem em sua composição Organismos Geneticamente Modificados (OGM), também denominados de transgênicos. São abordadas as convenções, as leis e as normas referentes a esses produtos dispostos no mercado, a adequação dos mesmos às normas vigentes e sua aceitação pela sociedade. Dispõe também sobre a importância do princípio da precaução na avaliação da aplicação de novas tecnologias ou de tecnologias das quais não se...

  1. Evolution of developments and applications of advanced thermal-hydraulics and neutronic codes. Conclusions from Annapolis Workshop and Ankara Seminar, Objectives of the Present Workshop

    International Nuclear Information System (INIS)

    In the nuclear reactor safety area, during the last 30-40 years, thermal-hydraulics has been one of the key disciplines for simulation and analysis of transient and accident scenarios and also for the definition of preventive and mitigative measures in relation to these scenarios. A workshop was organised by OECD/NEA-CSNI at Annapolis (1996) where codes, physical models, numeric and new computer architecture were examined. In parallel a Specialist meeting on instrumentation in two phase flows was held in Santa Barbara beginning of 1997 in order to investigate new techniques for getting measurements of new physical parameters necessary for assessing the new physical models. Among the different applications of thermal-hydraulic codes, the use of Best Estimate methods in safety evaluation is certainly one of the major challenges for which the safety and economic issues are quite important. For these reasons OECD/NEA-CSNI organised a seminar in Ankara in 1998 entirely devoted to the use of Best Estimate methods in thermal-hydraulics analysis. This seminar allowed to get a better view of where we were in such applications and which were the remaining problems and issues. The present workshop held in Barcelona beginning of year 2000 will be a good opportunity for providing an updated review of the gained progresses and for analysing if the objectives and programs are still progressing in the right direction. In order to do such exercise, we will first recall the questions which were raised in Annapolis and the main conclusions which were drawn from these questions. The conclusions of Ankara Meeting will be reviewed in a second step. Finally we will list the objectives of this workshop in Barcelona which is held in the continuity of Annapolis Workshop and Ankara Seminar. (authors)

  2. The Age and Geodynamic Evolution of the Metamorphic sole rocks from Izmir-Ankara-Erzıncan suture zone (Northern-Turkey)

    Science.gov (United States)

    Melih Çörtük, Rahmi; Faruk Çelik, Ömer; Özkan, Mutlu; Sherlock, Sarah C.; Marzoli, Andrea; Altıntaş, İsmail Emir; Topuz, Gültekin

    2016-04-01

    The İzmir-Ankara-Erzincan suture zone in northern Turkey is one of the major tectonic zones separating the Pontides to the North from the Anatolide-Tauride block and Kı rşehir Massif to the South. The accretionary complex of the İzmir-Ankara-Erzincan suture zone, near Artova, is composed mainly of peridotites with varying degree serpentinization, metamorphic rocks, basalt, sandstones, pelagic and neritic limestones. The metamorphic rocks are represented by amphibolite, garnet micaschit, calc-schist and marble. The metamorphic rocks were interpreted as the metamorphic sole rocks. Because; (i) They are tectonically located beneath the serpentinized peridotites. (ii) Foliation planes of both the amphibolites and mantle tectonites are parallel to each other. (iii) The metamorphic rocks are crosscut by non-metamorphic dolerite dikes which exhibite Nb and Ta depletion relative to Th enrichment on the N-MORB normalized multi-element spider diagram. The dolerite dikes display flat REE patterns (LaN/YbN=0.85-1.24). These geochemical signatures of the dolerite dikes are indicative of subduction component during their occurrences. Geochemical observations of the amphibolites suggest E-MORB- and OIB-like signatures (LaN/SmN= 1.39-3.14) and their protoliths are represented by basalt and alkali basaltic rocks. Amphiboles from the amphibolites are represented by calcic amphiboles (magnesio-hornblende, tchermakite and tremolite) and they yielded 40Ar-39Ar ages between 157.8 ± 3.6 Ma and 139 ± 11 Ma. These cooling ages were interpreted to be the intra-oceanic subduction/thrusting time of the İzmir-Ankara-Erzincan oceanic domain. This study was funded by TÜBİTAK (Project no: 112Y123).

  3. Petrogenetic Characteristics Of Oyaca Kedikayası Boyalık Adakites In SW Ankara (Central Anatolia, Turkey): Evidences For Slab Melt Metasomatism

    OpenAIRE

    Şen, Pınar; Şen, Erdal

    2013-01-01

    The Early Miocene Oyaca, Kedikayası and Boyalık dacites, situated approximately 50-60 km southwest of Ankara have affinities similar to adakitic rocks. They have porphyritic texture with a variable amount of plagioclase feldspar, hornblende and lesser biotite phenocrysts and a groundmass of plagioclase and quartz microcrysts. They have high Sr/Y (55-79 ppm) and (La/Yb)n (21-32 ppm) ratios, and low Y (10-19 ppm) and heavy rare earth element contents. According to their SiO2 (62.3-69.70 % wt.) ...

  4. PETROGENETIC CHARACTERISTICS OF OYACA – KEDİKAYASI – BOYALIK ADAKITES IN SW ANKARA (CENTRAL ANATOLIA, TURKEY): EVIDENCE FOR SLAB MELT METASOMATISM

    OpenAIRE

    Şen, Pınar; Şen, Erdal

    2013-01-01

    The Early Miocene Oyaca, Kedikayası and Boyalık dacites, situated approximately 50-60 km southwest of Ankara have affinities similar to adakitic rocks. They have porphyritic texture with a variable amount of plagioclase feldspar, hornblende and lesser biotite phenocrysts and a groundmass of plagioclase and quartz microcrysts. They have high Sr/Y (55-79 ppm) and (La/Yb)n (21-32 ppm) ratios, and low Y (10-19 ppm) and heavy rare earth element contents. According to their SiO2 (62.3-69.70 % wt.) ...

  5. Petrogenesis of the Refahiye Ophiolite and its Tectonic Significance for Neotethyan Ophiolites Along the İzmir-Ankara-Erzincan Suture Zone

    OpenAIRE

    SARIFAKIOĞLU, ENDER; WINCHESTER, HAYRETTİN ÖZEN & JOHN A.

    2009-01-01

    The Refahiye ophiolite, situated near Erzincan in the eastern part of the İzmir-Ankara-Erzincan Suture Zone (İAESZ), is one of the best exposures of oceanic lithosphere in the northern branch of the Neotethyan Ocean. The ophiolite, mainly thrust southwards, transported on an ophiolitic mélange, over the Early Triassic-Campanian Munzur Limestone of the Anatolide-Tauride Platform, was also emplaced by north-directed backthrusting onto the Pontides in the Late Cretaceous. It displays an almost c...

  6. An investigation on wind energy potential and small scale wind turbine performance at İncek region – Ankara, Turkey

    International Nuclear Information System (INIS)

    Highlights: • Weibull parameters for İncek region were calculated using five different methods. • Wind power density was calculated using the best representative Weibull parameters. • Energy generation of three small scale wind turbines were calculated. • Two wind turbines can supply yearly energy need of an average household in Turkey. - Abstract: Renewable energy resources increased their importance in the last decades due to environmental pollution problems. Additionally, the fact that fossil fuels such as oil, coal, and natural gas will be depleted in near future encourages researchers to make investigations on alternative energy resources. Wind energy, which is one of the most used alternative resources, has a great potential. In this study, Weibull parameters at İncek region of Ankara (the capital city of Turkey), where Atılım University campus is located, were determined for four different seasons and for twelve months in order to accomplish wind speed characterization at the region. Wind speed data at 20 m and 30 m heights were collected from a measurement station installed at Atılım University campus area. The data were taken as 1 min average values for a one year period between June 2012 and June 2013. Hourly average wind speed values for each height were derived using the collected wind data. Weibull parameters were calculated with five different methods using the derived hourly average wind speed values. According to root mean square error analyses, the best methods for which Weibull distribution fits the actual wind data were determined as power density and empirical methods. The power and energy density values for the region were also calculated for each season and each month. It was revealed that the maximum power density is encountered in March with about 98 (W/m2). Since this power density indicates that large scale wind turbine use is not a good option at the region, the performances of three different small scale wind turbines were

  7. Afforestation areas defined by GIS in Gölbaşi specially protected area Ankara/Turkey.

    Science.gov (United States)

    Dilek, E Figen; Sahin, Sükran; Yilmazer, Ilyas

    2008-09-01

    This paper concentrates on determining the areas for afforestation and the development of conservation strategies and actions in the case where there is high demand for the conservation of a particular land area. The analysis of hydrological landscape structure and of erodibility was prerequisite in the methodological design considering the site-specific natural landscape characteristics of the study area. The Gölbaşi Specially Protected Area (SPA) being the case area has many local environmental disturbances, especially with respect to its hydrological system, and the area is beset by increasing demands for its use by the burgeoning human population. It is obviously clear that the present intensive demand for recreational use will be forecasted further increase in the future. The count of rural residences is estimated to increase by 50% over the next decade. This intensive demand shall further strain the carrying capacity of the ecological milieu, which has already suffered to a significant degree because of the present urban and rural activities. The Gölbaşi district is located only 20 km from the city center of Ankara, and its surroundings were legally designated to be a Specially Protected Area primarily for the existence of lacustral and fluvial systems where two interconnected lakes exist: Mogan and Eğmir Lakes. Essentially, the continuity of the lacustral and fluvial system of Lakes Mogan and Eğmir depends on the preservation and improvement of the water sources. However, interconnected surface water bodies of these lakes are actually quite contaminated, and a decline in the water flow from Mogan to Eğmir has been observed. For the elaboration of the present method, required conventional maps (at 1/25,000 scale) of topography (for slope analysis) and geology (for erodibility and permeability analysis) were transferred to computer media using AutoCAD R.14 software. The rectification of the transferred data was done using ERDAS Imagine 8.3. All data

  8. Codelivery of Envelope Protein in Alum with MVA Vaccine Induces CXCR3-Biased CXCR5+ and CXCR5- CD4 T Cell Responses in Rhesus Macaques.

    Science.gov (United States)

    Iyer, Smita S; Gangadhara, Sailaja; Victor, Blandine; Gomez, Rosy; Basu, Rahul; Hong, Jung Joo; Labranche, Celia; Montefiori, David C; Villinger, Francois; Moss, Bernard; Amara, Rama Rao

    2015-08-01

    The goal of an HIV vaccine is to generate robust and durable protective Ab. Vital to this goal is the induction of CD4(+) T follicular helper (TFH) cells. However, very little is known about the TFH response to HIV vaccination and its relative contribution to magnitude and quality of vaccine-elicited Ab titers. In this study, we investigated these questions in the context of a DNA/modified vaccinia virus Ankara SIV vaccine with and without gp140 boost in aluminum hydroxide in rhesus macaques. In addition, we determined the frequency of vaccine-induced CD4(+) T cells coexpressing chemokine receptor, CXCR5 (facilitates migration to B cell follicles) in blood and whether these responses were representative of lymph node TFH responses. We show that booster modified vaccinia virus Ankara immunization induced a distinct and transient accumulation of proliferating CXCR5(+) and CXCR5(-) CD4 T cells in blood at day 7 postimmunization, and the frequency of the former but not the latter correlated with TFH and B cell responses in germinal centers of the lymph node. Interestingly, gp140 boost induced a skewing toward CXCR3 expression on germinal center TFH cells, which was strongly associated with longevity, avidity, and neutralization potential of vaccine-elicited Ab response. However, CXCR3(+) cells preferentially expressed the HIV coreceptor CCR5, and vaccine-induced CXCR3(+)CXCR5(+) cells showed a moderate positive association with peak viremia following SIV251 infection. Taken together, our findings demonstrate that vaccine regimens that elicit CXCR3-biased TFH cell responses favor Ab persistence and avidity but may predispose to higher acute viremia in the event of breakthrough infections. PMID:26116502

  9. Degradación in-vitro de mezclas de poliuretano termoplástico y almidón modificado In-vitro degradation of thermplastic polyurethane and starch modified

    OpenAIRE

    Manuel F. Valero

    2013-01-01

    La estructura química del almidón de yuca se modificó mediante una reacción de acilación. El almidón de yuca modificado se mezcló con un prepolímero de uretano obtenido a partir de aceite de ricino modificado por transesterificación (MCO) y diisocianato de isoforona (IPDI) con el fin de preparar mezclas de almidón-poliuretano termoplástico. Los poliuretanos fueron expuestos a degradación in-vitro durante un período de 365 días y se evaluó la pérdida de masa y el cambio de las propiedades mecá...

  10. Características físico-químicas e utilização em alimentos de amidos modificados por tratamento oxidativo Obtention, physicochemical characterization and applications in foods of starches modified by oxidative treatment

    OpenAIRE

    Marianne Ayumi Shirai; Ângela Haas; Gustavo Fattori Ferreira; Luciana Shizue Matsuguma; Célia Maria Landi Franco; Ivo Mottin Demiate

    2007-01-01

    A utilização de amidos modificados em alimentos tem sido uma alternativa para melhorar as características que os amidos nativos não podem conferir. Os objetivos deste trabalho foram: avaliar características físico-químicas de amidos modificados com peróxido de hidrogênio, utilizar os amidos obtidos para a elaboração de doce de leite e bala de goma americana e verificar a aceitação sensorial destes produtos. Para obtenção das amostras modificadas, os amidos foram suspensos em solução de Fe2SO4...

  11. Siembra comercial de cultivos genéticamente modificados en el campo mexicano: una herramienta viable para el desarrollo rural sustentable y la seguridad alimentaria

    Directory of Open Access Journals (Sweden)

    Alejandro Monteagudo Cuevas

    2014-01-01

    Full Text Available Hacia las últimas décadas del siglo pasado, ciertas técnicas de ingeniería gené- tica empezaron a aplicarse a la agricultura, dando origen a la biotecnología agrícola moderna. Esta ciencia se orientó hacia fines tan importantes como el combate a las plagas que azotan varios cultivos de gran importancia económica y a la tolerancia a herbicidas. Gracias a los avances de la ingeniería genética, el mundo está logrando grandes beneficios en la agricultura, y en consecuencia en la alimentación de la hu- manidad. Au nque los organismos genéticamente modificados ( ogm tienen apenas 18 años de ser sembrados, son ya la tecnología agrícola de más rápida adopción en la historia.

  12. El Pennhip modificado y la sinfisiodesis juvenil pubiana como prevención de displasia de cadera canina en las Fuerzas Armadas

    Directory of Open Access Journals (Sweden)

    C. Sánchez Alonso

    2015-09-01

    Full Text Available Introducción: La displasia de cadera canina es la principal causa física de exclusión de los perros de los programas de adiestramiento en las Fuerzas Armadas y los Cuerpos y Fuerzas de Seguridad del Estado, es por ello importante realizar una criba temprana de esta patología. Objetivo: Determinar la eficacia del procedimiento del Penn-Hip modificado y la sinfisiodesis juvenil pubiana como método para prevenir el desarrollo de la displasia de cadera canina. Material y método: Estudio descriptivo y ambispectivo que compara el porcentaje de displasia de cadera canina en una cohorte prospectiva, tras la realización de un programa de screening y cirugía profiláctica, con los resultados de una cohorte histórica. Resultados: El porcentaje de displasia de cadera canina fue el doble en la cohorte histórica comparándola con la cohorte prospectiva tras la implantación del programa, siendo las diferencias estadísticamente significativas (OR: 2,20; IC 95%: 1,03-4,67. Conclusiones: Es necesaria la instauración de programas de screening y profilaxis de displasia de cadera canina, en el ámbito militar al mejorar la producción de perros aptos para el trabajo en las Fuerzas Armadas y en los Cuerpos y Fuerzas de Seguridad del Estado.

  13. A falta de informação sobre os Organismos Geneticamente Modificados no Brasil The lack of information on Genetically Modified Organisms in Brazil

    Directory of Open Access Journals (Sweden)

    Isabelle Geoffroy Ribeiro

    2012-02-01

    Full Text Available O presente artigo apresenta uma revisão sobre a rotulagem de produtos que apresentem em sua composição Organismos Geneticamente Modificados (OGM, também denominados de transgênicos. São abordadas as convenções, as leis e as normas referentes a esses produtos dispostos no mercado, a adequação dos mesmos às normas vigentes e sua aceitação pela sociedade. Dispõe também sobre a importância do princípio da precaução na avaliação da aplicação de novas tecnologias ou de tecnologias das quais não se conhece ou existam conhecimentos científicos relevantes quanto aos seus potenciais riscos ao meio ambiente, à saúde humana e à sociedade.This article presents a review about the labeling of products that have Genetically Modified Organisms (GMO, also called transgenic elements in their composition. It addresses the conventions, laws and regulations relating to such products currently governing the market, the adequacy of these existing standards and their acceptance by society. It also examines the importance of the cautionary principle when assessing the application of new technologies or technologies where little is known or where there is no relevant scientific knowledge about the potential risks to the environment, human health and society.

  14. Influência de Negro de Fumo Modificado com Polianilina na Estrutura de Compósitos com Poli(Fluoreto de Vinilideno

    Directory of Open Access Journals (Sweden)

    Zucolotto Valtencir

    2002-01-01

    Full Text Available Compósitos condutores elétricos a base de poli(fluoreto de vinilideno (PVDF e negro de fumo modificado com polianilina (Eeonomer® foram preparados através do processamento por fusão. Resultados de reometria de torque indicaram que a presença de polianilina no negro de fumo é vantajosa ao processamento por fusão, reduzindo a viscosidade do fundido em comparação com a aditivação com negro de fumo puro. Compósitos contendo acima de 5% em peso de Eeonomer® apresentaram condutividade da ordem de 10-2 S/cm. Esse valor se manteve estável mesmo após tratamento térmico a 150degreesC por 170 h em atmosfera ambiente. Análises por calorimetria diferencial de varredura (DSC e difração de Raios-X mostraram que o tipo e a quantidade de Eeonomer®, assim como a taxa de resfriamento da amostra, podem afetar a temperatura de fusão, o grau de cristalinidade e a fase cristalina do PVDF presente no compósito.

  15. Comparison of Corporate Image a nd Patient Loyalty Perceptions of Outpatients and Inpatients: Example of a Training and Research Hospital in Ankara

    Directory of Open Access Journals (Sweden)

    Ömer Rıfkı Önder

    2014-12-01

    Full Text Available The purpose of this study is to determine the level of corporate image and patient loyalty of outpatients and inpatients who get services from a hospital and to evaluate the relationship between corporate images’ factors and patient loyalty. Totally 600 patients from a training and research hospitals in Ankara, formed the study sample. As a result, outpatients’ loyalty and image perceptions found medium level; while inpatients’ level found high. In addition, the effect of corporate image factors on patient loyalty was determined that there is a statistically significant , strong and positive correlation and 83% of patient loyalty is explained by corporate image factors. Based on the research findings, making improvements especially in quality and also physical, communication, social responsibility factors can obtain loyal patients. It is suggested to adopt different strategies to outpatients and inpatients while implementing these improvements.

  16. Ankara’daki Halk Kütüphanelerinde Halkla İlişkiler =Public Relations of Public Libraries in Ankara

    Directory of Open Access Journals (Sweden)

    Karadeniz, Şenol

    Full Text Available Bu çalışmada Ankara'daki halk kütüphanelerinin, kullanıcılarını sunulan hizmetler hakkında neden yeterince bilgilendiremedikleri ve kendilerini kullanıcılarına neden yeterince tanıtamadıkları konusu irdelenmiştir. Araştırmanın amacı, halk kütüphanelerinde uygulanabilecek halkla ilişkiler yöntemlerinin neler olduğunu, halk kütüphanelerinin planlı halkla ilişkiler faaliyetlerinde bulunmamalarının nedenlerini ve bu nedenlerin boyutunu, özelliklerini ortaya koymaktır. Araştırmanın evrenini Ankara Büyükşehir Belediyesi sınırları içinde hizmet veren Kültür ve Turizm Bakanlığı'na bağlı halk ve çocuk kütüphaneleri oluşturmaktadır. Önceden yapılandırılmış görüşme kapsamında halk kütüphanesi yöneticileriyle görüşülmüş, ayrıca araştırma kapsamındaki kütüphanelerde gözlem tekniği uygulanmıştır. Araştırmadan elde edilen bulgular ışığında, halk kütüphanelerinin sistematik halkla ilişkiler programları uygulamamaları nedeniyle, kütüphaneyi tanıtmada ve sunulan hizmetlere ilişkin kullanıcılarda olumlu görüş oluşturmada yetersiz kaldıkları anlaşılmıştır In this study the answers to the question "why the public libraries in Ankara could not inform their users about services off ered properly, and could not introduce themselves to users suffi ciently ?" were explicated. The goal of the study is to fi nd out which public relations methods can be used in libraries, and why libraries could not conduct planned public relations activities. The research universe consists of public libraries being administratively connected to Ministry of Culture and Tourism, which lie within the borders of Ankara Metropolitan Municipality. For the collection of data, pre-confi gured talks were applied to the managers who were working for the public libraries, and moreover the fulfi llment of library activities were observed. The fi ndings of the study indicate that, since

  17. The Impac t of Managers’ Leadership Behaviors on Job Satisfaction of Employees: A Practice Used In The Lodging Facilities In Ankara

    Directory of Open Access Journals (Sweden)

    Arzu Kılıçlar

    2014-09-01

    Full Text Available The aim of this research, the impact of the managers' leadership behaviours on the employees' job satisfaction. For that purpose, it has been reachedall of the 14, 5 stars accommodation enterprices located in Ankara. From the employeesin these enterprices, datas are collected with the questionnaire formand in total 386 questionnaires are taken into consideration. The forms have 3 chapters. In the first chapter demographic datas are found, in the second chapter, the expressions to measure the managers' leadership behaviours are found, and in the thirdone, the expressions to measure the job satisfaction level of theemployeesare found. All these expressions are filled by the employees. Correlation analysis t-test and ANOVA was performed by using the data obtained from the questionnaire. A sall the datas are analysed, it is found that, there is a positiveway of meaningful relationship between the managers' leadership behavior and the employees' job satisfaction level.

  18. ANKARA ÜNİVERSİTESİ ZİRAAT FAKÜLTESİ YONCA EKİM ALANLARINDA GÖRÜLEN ÖNEMLİ HASTALIKLARIN BELİRLENMESİ

    OpenAIRE

    ONAR, Mihrace Çiğdem

    2005-01-01

    Bu çalışma ile Ankara Üniversitesi Ziraat Fakültesi Ayaş ve Haymana çiftlikleri ile Dışkapı yerleşkesindeki yonca ekim alanlarındaki önemli hastalıklar belirlenmiştir. 2003 - 2004 yıllarında belirtilen bölgelerden örnekler alınarak hastalık etmenlerinin teşhisleri yapılmıştır. Teşhisi yapılan funguslar; Leptotrochila medicaginis, Pseudopeziza medicaginis, Leveillula taurica, Peronospora trifoliorum, Phoma medicaginis var. medicaginis, Colletotrichum trifolii, Stemphylium botryosum, Stagon...

  19. Residents’ Awareness of Urban and Social Living Quality: The Example of Prof. Dr. Ahmet Taner Kışlalı Neighborhood in Çayyolu/Ankara

    Directory of Open Access Journals (Sweden)

    Ahmet Tanju Gültekin

    2015-12-01

    Full Text Available Since 1980, the dynamics of globalization have directed the processes of social, cultural, and political adjustment as well as those of economic integration and especially capital towards urban development, forming urban space. The wave of globalization in Turkey, within a process of economic change in the context of neo liberal policies, urban space has been turned into the most profitable area of investment in the unearned income (economic rent sector. The residential sector has been included in this process quickly and easily through effective and widespread marketing strategies. Over the last two decades of this development, the Ankara-Çayyolu District has become segregated economically and socially between its upper and upper-middle class residents in the urban context. This segregation is evident in residential areas with high-level physical and spatial quality. On the other hand, in addition to the measurable physical and spatial standards of housing quality, it is also important to find out if the quality of living meets the social and cultural expectations of its residents. Starting from this point of view, we examine residents’ perceptions of their physical and social environment in our chosen sample area in the Prof. Dr. Ahmet Taner Kışlalı Neighborhood, and their demands and awareness of housing quality. The story of this neighborhood, which begins in 1980, at a time when neo liberal policies and the power to form urban space was all-important, is defined here by the residents’ quality of living and awareness of it. Our results obtained are expected to help guide the creation of liveable housing areas in the future.Keywords: Housing Users, Urban Living Quality, Social Living, Awareness, Ankara- Çayyolu-Prof. Dr. Ahmet Taner Kışlalı Neighborhood.

  20. Development of cooling system for 66/6.9kV-20MVA REBCO superconducting transformers with Ne turbo-Brayton refrigerator and subcooled liquid nitrogen

    Science.gov (United States)

    Iwakuma, M.; Adachi, K.; Yun, K.; Yoshida, K.; Sato, S.; Suzuki, Y.; Umeno, T.; Konno, M.; Hayashi, H.; Eguchi, T.; Izumi, T.; Shiohara, Y.

    2015-12-01

    We developed a turbo-Brayton refrigerator with Ne gas as a working fluid for a 3 ϕ- 66/6.9kV-2MVA superconducting transformer with coated conductors which was bath-cooled with subcooled LN2. The two-stage compressor and expansion turbine had non-contact magnetic bearings for a long maintenance interval. In the future, we intend to directly install a heat exchanger into the Glass-Fiber-Reinforced-Plastics cryostat of a transformer and make a heat exchange between the working fluid gas and subcooled LN2. In this paper we investigate the behaviour of subcooled LN2 in a test cryostat, in which heater coils were arranged side by side with a flat plate finned-tube heat exchanger. Here a He turbo-Brayton refrigerator was used as a substitute for a Ne turbo-Brayton one. The pressure at the surface of LN2 in the cryostat was one atmosphere. Just under the LN2 surface, a stationary layer of LN2 was created over the depth of 20 cm and temperature dropped from 77 K to 65 K with depth while, in the lower level than that, a natural convection flow of LN2 was formed and temperature was almost uniform over 1 m depth. The boundary plane between the stationary layer and the natural convection region was visible.

  1. Co-administration of Interleukin-2 Enhances Cellular and Humoral Immune Responses to HIV Vaccine DNA Prime/MVA Boost Regime

    Institute of Scientific and Technical Information of China (English)

    JIANG Chun-lai; YU Xiang-hui; WU Yong-ge; LI Wei; KONG Wei

    2005-01-01

    Interleukine-2(IL-2) is a growth factor for antigen-stimulated T lymphocytes and is responsible for T-cell clonal expansion after antigen recognition. It has been demonstrated that DNA vaccine-elicited immune responses in mice could be augmented substantially by using either an IL-2 protein or a plasmid expressing IL-2. Twenty mice, divided into four experimental groups, were immunized with: (1) sham plasmid; (2) HIV-1 DNA vaccine alone; (3) HIV-1 DNA vaccine and IL-2 protein; or (4) HIV-1 DNA vaccine and IL-2 plasmid, separately. All the groups were immunized 3 times at a 2-week interval. Fourteen days after the last DNA vaccine injection, recombinant MVA was injected into all the mice except those in group 1. ELISA and ELISPOT were employed to investigate the effect of IL-2 on DNA vaccine immune responses. The obtained results strongly indicate that the efficacy of HIV vaccine can be enhanced by co-administration of a plasmid encoding IL-2.

  2. Gene Expression Driven by a Strong Viral Promoter in MVA Increases Vaccination Efficiency by Enhancing Antibody Responses and Unmasking CD8+ T Cell Epitopes

    Directory of Open Access Journals (Sweden)

    Pablo D. Becker

    2014-07-01

    Full Text Available Viral vectors are promising tools for vaccination strategies and immunotherapies. However, CD8+ T cell responses against pathogen-derived epitopes are usually limited to dominant epitopes and antibody responses to recombinant encoded antigens (Ags are mostly weak. We have previously demonstrated that the timing of viral Ag expression in infected professional Ag-presenting cells strongly shapes the epitope immunodominance hierarchy. T cells recognizing determinants derived from late viral proteins have a clear disadvantage to proliferate during secondary responses. In this work we evaluate the effect of overexpressing the recombinant Ag using the modified vaccinia virus early/late promoter H5 (mPH5. Although the Ag-expression from the natural promoter 7.5 (P7.5 and the mPH5 seemed similar, detailed analysis showed that mPH5 not only induces higher expression levels than P7.5 during early phase of infection, but also Ag turnover is enhanced. The strong overexpression during the early phase leads to broader CD8 T cell responses, while preserving the priming efficiency of stable Ags. Moreover, the increase in Ag-secretion favors the induction of strong antibody responses. Our findings provide the rationale to develop new strategies for fine-tuning the responses elicited by recombinant modified vaccinia virus Ankara by using selected promoters to improve the performance of this viral vector.

  3. Modelo de Whiten (1972 modificado para modelación de la trituración de la antracita residual de Nicaro

    Directory of Open Access Journals (Sweden)

    Andrey Leyva-Mormul

    2015-05-01

    Full Text Available Con el objetivo de modelar el proceso de trituración del carbón antracita en trituradores de cono se tomó como punto de partida el modelo de Whiten sustituyendo las funciones de clasificación y de distribución de la fragmentación por las ecuaciones de Whiten y White (1979 y Austin y Luckie (1972. Para la determinación de ambas funciones y la identificación de sus coeficientes de forma conjunta fue utilizado un algoritmo desarrollado por Hechavarría (2011. Los resultados mostraron que la función de clasificación coincide con la forma de la función cuadrática reportada por Vogel y Peukert (2005 y Coello et al. (2011. La función de distribución de la fragmentación acumulativa para las diferentes clases de tamaño obtenida confirma la validez del modelo teórico propuesto por Austin y Luckie. La composición granulométrica del producto triturado obtenida por el modelo sigue patrones similares a la curva granulométrica obtenida por el muestreo en la instalación estudiada. Los valores del coeficiente de correlación (0,9826 y del estadígrafo de Chi-cuadrado confirma la validez del modelo de Whiten modificado para la modelación de la trituración de la antracita de la instalación del Centro de Investigaciones Siderúrgicas de Nicaro.

  4. Pesquisa de oocistos de Cryptosporidium sp em fezes: comparação entre os métodos de Kinyoun modificado e de heine

    Directory of Open Access Journals (Sweden)

    Vicente Amato Neto

    1996-12-01

    Full Text Available Para diagnosticar a infecção intestinal pelo Cryptosporidium sp, hoje bastante em foco pela não incomum participação no contexto da síndrome da imunodeficiência adquirida (AIDS, várias técnicas têm sido indicadas. Com o intuito de esclarecer vantagens e desvantagens, envolvendo quantificação, morfologia, durabilidade do esfregaço e tempo de preparação, comparamos os resultados obtidos mediante emprego de dois desses processos, ou seja, de Kinyoun modificado e de Heine, aplicados em amostras fecais de pacientes com AIDS. A sensibilidade foi bastante semelhante e a escolha deve depender da valorização das virtudes de cada uma das técnicas, que são: quanto â de Kinyoun modificada, a durabilidade dos esfregaços e, a propósito da de Heine, a rapidez com que se dã o preparo, aliada â superioiidade quando o parâmetro é a quantidade de oocistos encontrados, mais freqüentemente verificada.The diagnosis of intestinal infection by Cryptosporidium sp is crucial today; with the progression of the AIDS epidemic, many cases of cryptosporidiosis have appeared in this setting and in other immunodeficiency diseases. We compared the advantages and disadvantages of Heine's method and modified Kinyoun's method in the following parameters: morphology of the parasite, quantitation of cysts, stability of the staining characteristics timewise on the slides and time spend at staining. All positive fecal smears were obtained from patients with AIDS. The sensitivity of these two techniques was the same. The choice should be made by the best aspects of each method. Heine's was better for quantitation of the cysts and was faster. Kinyoun's was better for conserving the stained smear.

  5. Características físico-químicas e utilização em alimentos de amidos modificados por tratamento oxidativo Obtention, physicochemical characterization and applications in foods of starches modified by oxidative treatment

    Directory of Open Access Journals (Sweden)

    Marianne Ayumi Shirai

    2007-06-01

    Full Text Available A utilização de amidos modificados em alimentos tem sido uma alternativa para melhorar as características que os amidos nativos não podem conferir. Os objetivos deste trabalho foram: avaliar características físico-químicas de amidos modificados com peróxido de hidrogênio, utilizar os amidos obtidos para a elaboração de doce de leite e bala de goma americana e verificar a aceitação sensorial destes produtos. Para obtenção das amostras modificadas, os amidos foram suspensos em solução de Fe2SO4.7H2O 0,01% e, em seguida, adicionou-se peróxido de hidrogênio 1,25%, ajustou-se o pH para 3,0, deixou-se reagir a 45 °C/15 minutos, lavou-se e secou-se a amostra em estufa de ventilação forçada a 45 °C/24 horas. Os amidos modificados apresentaram maior expansão, conteúdo de carboxilas, poder redutor e susceptibilidade à sinérese. Em relação à viscosidade aparente, verificou-se que com a modificação química houve diminuição no pico de viscosidade, especialmente quando as amostras foram analisadas em pH 7,0. A análise sensorial do doce de leite revelou que houve preferência pelas amostras elaboradas com amido de mandioquinha-salsa modificado e com o amido de milho regular ácido-modificado. A bala de goma produzida com amido de mandioquinha-salsa modificado ocupou o segundo lugar entre a preferência dos julgadores.The use of modified starches in foods is made to improve characteristics that the native cannot confer. The objective of this work was to evaluate physicochemical characteristics of starches from several botanical sources after chemical modification with H2O2 considering technological properties of interest to the food industry. The modified starches were used in dulce de leche and starch gum and the sensorial acceptance of these products was verified. The chemical treatment included the suspension of the native starches in a 0.01% Fe2SO4, followed by addition of 1.25% H2O2 at pH 3.0 and reaction time of 15 min

  6. Características físico-químicas de amidos modificados de grau alimentício comercializados no Brasil Physicochemical characteristics of modified food starches commercialized in Brazil

    Directory of Open Access Journals (Sweden)

    Graziela de Oliveira da Silva

    2006-03-01

    Full Text Available Amidos nativos e modificados têm grande importância na indústria de alimentos, sendo empregados principalmente como espessantes e/ou estabilizantes. As limitações das pastas e géis obtidos a partir de amidos nativos tornaram necessário o desenvolvimento de muitos tipos de amidos modificados para aplicações alimentícias. Neste trabalho, algumas amostras de amidos modificados (n=20 disponíveis no Brasil foram recebidas de empresas produtoras e analisadas em relação a algumas características físico-químicas e propriedades tecnológicas. Um levantamento do uso de amidos modificados em alimentos também é apresentado, revelando crescente interesse pela indústria nesses ingredientes. Constatou-se que, enquanto alguns alimentos industrializados, como maioneses contêm em suas formulações amidos modificados, outros como condimento preparado de mostarda contêm apenas amido nativo. As análises físico-químicas permitiram concluir que alguns amidos modificados apresentavam teores elevados de acidez, relacionados à presença de reagentes utilizados em sua obtenção, não havendo presença de carboxilas nas suas macromoléculas. De maneira geral, os resultados de algumas propriedades tecnológicas avaliadas, tais como viscosidade aparente das pastas, resistência a congelamento/descongelamento e propriedade de expansão, estavam de acordo com a descrição dos produtos. As fontes mais observadas nas modificações foram, em ordem decrescente de importância, a mandioca, o milho ceroso e o milho regular.Native and modified starches are very valued for the food industry, being employed mainly as thickeners or stabilizers. Several technological limitations of native starch pastes or gels made it necessary to develop modified starches for the food industry. In the present work modified starch samples (n=20 available in Brazil were received from some producers and evaluated in relation to selected physicochemical characteristics and

  7. Ankara Kedilerinde (Felis catus angorensis) dış yapı, tüy, büyüme, gelişme ve üreme özellikleri üzerine araştırmalar

    OpenAIRE

    ÖZÇETİN, Sebahattin Tarkan

    2007-01-01

    Ankara Atatürk Orman Çiftliği (A.O.Ç.) Hayvanat Bahçesinde yetiştirilen 5 erkek, 28 dişi ve bu kedilerden doğan 70  yavru  ile  Ankara’nın  çeşitli  semtlerinde  bakılan  11  erkek, 10 dişi, 4 kastre erkek ve 9 steril dişi olmak üzere toplam 137 Ankara Kedisinin kullanıldığı   çalışmada  Ankara Kedisinin dış yapı, tüy, büyüme, gelişme ve üreme özellikleri incelenmiştir. Ankara A.O.Ç. Ha...

  8. Degradación in-vitro de mezclas de poliuretano termoplástico y almidón modificado In-vitro degradation of thermplastic polyurethane and starch modified

    Directory of Open Access Journals (Sweden)

    Manuel F. Valero

    2013-01-01

    Full Text Available La estructura química del almidón de yuca se modificó mediante una reacción de acilación. El almidón de yuca modificado se mezcló con un prepolímero de uretano obtenido a partir de aceite de ricino modificado por transesterificación (MCO y diisocianato de isoforona (IPDI con el fin de preparar mezclas de almidón-poliuretano termoplástico. Los poliuretanos fueron expuestos a degradación in-vitro durante un período de 365 días y se evaluó la pérdida de masa y el cambio de las propiedades mecánicas y térmicas. El objetivo de este estudio fue examinar el efecto de la incorporación de almidón modificado por acilación en el poliuretano sobre las propiedades mecánicas, térmicas y la velocidad de degradación.The chemical structure of the cassava starch was modified by acylation. Cassava starch modified was mixed with urethane prepolymer based on castor oil modified by transesterification (MCO and isophorone diisocyanate (IPDI in order to prepare starch-blended thermoplastic polyurethanes. Polyurethanes were exposed to in-vitro degradation then monitored for changes in mass and mechanical and thermal properties over a period of 365 days. The effect of starch modified content on the thermal and physical properties of the resulting materials was detailed investigated by dynamic mechanical thermal analysis (DMTA and measurements of mechanical properties. The aim of this study was to examine the effect of introducing starch modified into the polyurethane on the properties and in-vitro degradation.

  9. Estudio comparativo de la ozonización de aceites de girasol modificados genéticamente y sin modificar Comparative study of the ozonation of genetically modified and not modified sunflower oils

    OpenAIRE

    Díaz Gómez, Maritza F.; Oscar E. Ledea Lozano; Magali Gómez Regüeiferio; Rafael Garcés Mancheño; Manuel S. Alaiz Barragán; Enrique Martínez Force

    2009-01-01

    [ES] Se han desarrollado diferentes estudios sobre la composición química y microbiológica de diferentes aceites vegetales ozonizados tales como, el girasol. 13-16 Sin embargo, no hemos encontrado ningún estudio realizado en los aceites vegetales modificados genéticamente tratado con ozono, los cuales pudieran sustituir al aceite de girasol ozonizado, debido a su menor precio en el mercado. Por lo que es objetivo de este trabajo la comparación química y microbiológi...

  10. Static electrification testing of Texas Utilities 345-20.9 kV, 650 MVA generator step-up transformer under controlled factory conditions

    International Nuclear Information System (INIS)

    Comanche Peak Nuclear Power Plant, with two 1150 Megawatt units, has two 345-20.9 kV, three-phase, 650 MVA transformers connected in parallel. The transformers have had a long history of hydrogen generation, and one of the Unit No. 2 transformers failed in 1983 while energized from the system for plant construction power. The failed unit was repaired and returned to Comanche Peak, and there was no evidence of static electrification involvement in the failure. During the time from installation to commercial operation in 1990 for Unit No. 1 and 1992 for Unit No. 2, the transformers were backfed from the system as needed to provide construction power. There was little regard paid to the operation of the cooling systems other than assuring the cooling was running. The transformers on unit No. 1 were subjected to several unusual electrical events - ferroresonance in 1981 and over excitation in 1983, with the generation of gasses of great concern. The decision was made to install a new transformer and return the old unit to the factory for diagnostic testing to determine the cause for the generation of combustible gas, primarily hydrogen. The dielectric integrity of the transformer could be quantified and decisions made concerning the replacement of the remaining transformers. After testing, the transformer was disassembled for forensic evaluation and rebuilt for return as a spare or to replace another transformer while undergoing repairs. Deformation of the bottom of the tank was discovered with large dents in the bottom protruding inward approximately one and one-half inches. With the concern of decreased clearances or displaced flux shields in the tank, an inspection opening was cut in the end of the transformer near the bottom. While investigating the deformation, evidence of partial discharge activity was discovered at the bottom of the high-to-low insulation; a location where static electrification damage has been observed in other large shell-form transformers

  11. Ankara Çubuk yöresi turşularından izole edilen laktik asit bakterilerinin bazı teknolojik ve fonksiyonel özelliklerinin belirlenmesi

    OpenAIRE

    ARSLANKOZ, Nurdan

    2011-01-01

          Bu çalışmada, Ankara Çubuk bölgesi turşularından izole edilerek tanımlanması yapılmış ve Ankara Üniversitesi Mühendislik Fakültesi Gıda Mühendisliği Bölümü Kültür Koleksiyonu'nda bulunan 36 adet laktik asit bakterisinin bazı teknolojik ve fonksiyonel özellikleri belirlenmiştir.

    36 adet laktik asit bakterisi suşunun farklı pH ve tuz değerlerinde gelişmesi, laktik asit, H2O2

  12. Modified E-test by the addition of EDTA-Tris and dimethyl sulfoxide on the potentiation of the effects of some antimicrobials in Pseudomonas aeruginosa strains isolated from bovine mastitis E-test modificado pela adição de Tris-EDTA e dimetilsulfóxido na potencialização do efeito de antimicrobianos em linhagens de Pseudomonas aeruginosa isoladas de mastite bovina

    Directory of Open Access Journals (Sweden)

    M.G. Ribeiro

    2004-10-01

    Full Text Available A concentração inibitória mínima-MIC em 30 estirpes de Pseudomonas aeruginosa isoladas de mastite bovina foi avaliada utilizando o E-test padrão e o método modificado, pela adição de Tris-EDTA e DMSO. Os métodos modificados apresentaram redução significativa da MIC das estirpes utilizando a gentamicina, a ciprofloxacina e a norfloxacina.

  13. Ankara Üniversitesi Ziraat Fakültesi yonca ekim alanlarında görülen önemli hastalıkların belirlenmesi

    OpenAIRE

    KARAKAYA, Aziz; ONAR, Mihrace Çiğdem

    2006-01-01

    In this study, important alfalfa diseases occurring in the Ankara University Faculty of Agriculture Haymana and Ayaş Research Farms and Dışkapı Campus were determined. Samples were collected during the years 2003 and 2004 and causal agents of the diseases were identified. The fungi found were: Phoma medicaginis var. medicaginis, Leptotrochila medicaginis, Leveillula taurica, Pseudopeziza medicaginis, Peronospora trifoliorum, Stemphylium botryosum, Stagonospora meliloti, Colletotrichum trifoli...

  14. The Profitability of Animal Husbandry Activities on Farms in Dry Farming Areas and the Interaction between Crop Production and Animal Husbandry: The Case of Ankara Province in Turkey

    Directory of Open Access Journals (Sweden)

    Harun Tanrıvermis

    2007-04-01

    Full Text Available This paper examines the linkages between livestock and crop farming activities and provides a comparative analysis of the profitability of different livestock activities in the highlands of Ankara. The data was collected from 52 sample farms in the Nallıhan, Aya¸s, Güdül and Beypazarı districts of Ankara by way of a questionnaire, where the farms have, on average, 20.7 ha of land and are thus regarded as small family farms. Insufficient irrigated land and working capital, weak market relations and the pressure of high population brings about a requirement to strengthen crop-livestock interaction. Production on the farms is generally carried out in extensive conditions, with goat, sheep and cattle husbandry in addition to crop production. Crop production makes up for 20.8% of the total gross production value on the farms. Of this figure, the entire yields of wheat, barley, pulses, straw and fodder crops are used for own consumption by the households, along with 74% of the wheat and 77% of the barley produced. The research results indicate that the current management systems may be defined as mixed farms in terms of crop–livestock linkages. The average total income of the households surveyed is 9,412.0 USD, of which 63.4% comes from farming activities. Every 1 USD invested in animal husbandry provides an income of 1.12 USD from dairy cattle breeding, 1.13 USD from Angora goat breeding, 1.16 USD from sheep breeding and 1.27 USD from ordinary goat breeding. It has been found that ordinary goat breeding, which provides the greatest relative profitability for the farms, offers many advantages, and that the transition from Angora goat breeding to ordinary goat breeding through the breeding of ordinary male goats into the Angora herd has occurred in recent years. The results of the survey indicate that supporting crop production with animal husbandry is considered a requirement in order to maintain economic and social sustainability in the farms

  15. Aplicabilidade da metodologia de reação de polimerase em cadeia em tempo real na determinação do percentual de organismos geneticamente modificados em alimentos Applicability of the real-time polymerase chain reaction based-methods in quantification of genetically modified organisms in foods

    OpenAIRE

    Natália Eudes Fagundes de Barros; Edna Maria Morais Oliveira; Victor Augustus Marin

    2008-01-01

    A detecção de organismos geneticamente modificados na cadeia alimentar é um aspecto importante para todos os assuntos envolvidos no controle de matéria-prima, na indústria de alimentos e na distribuição. A rotulagem e a rastreabilidade de organismos geneticamente modificados são questões atuais que são consideradas para o comércio e a regulamentação. Atualmente, a rotulagem de alimentos processados contendo material transgênico detectável é exigida pela legislação brasileira. O governo brasil...

  16. Uso do índice de Kotelchuck modificado na avaliação da assistência pré-natal e sua relação com as características maternas e o peso do recém-nascido no Município do Rio de Janeiro

    OpenAIRE

    Maria do Carmo Leal; Silvana Granado Nogueira da Gama; Katia Maria Netto Ratto; Cynthia Braga da Cunha

    2004-01-01

    O índice de Kotelchuck (IK) foi modificado e utilizado para avaliar a assistência pré-natal prestada no Município do Rio de Janeiro - Brasil, em uma amostra de 9920 puérperas de parto único. Regressões multivariadas logística ordinal (RMLO) e linear (RML) foram utilizadas para estimar a importância de fatores demográficos, psicossociais e obstétricos no IK modificado e seus efeitos no peso ao nascer (PN), respectivamente. Apenas 38,5% das parturientes do Município do Rio de Janeiro foram clas...

  17. Adsorção de nitrato em caulinita a partir de rejeito de caulim modificado com uréia

    Directory of Open Access Journals (Sweden)

    L. C. Tavares

    2013-12-01

    Full Text Available Este estudo avalia a eficiência, viabilidade e espontaneidade de processos de adsorção do íon nitrato em caulinita sem tratamento e modificada com uréia, empregando como material de partida o rejeito de processamento de caulim do rio Jari. Os materiais foram caracterizados por difração de raios X, fluorescência de raios X e espectroscopia IV (DRX, FRX e FTIR. As medidas de pH de equilíbrio foram efetuadas por potenciometria e as concentrações de equilíbrio (Ce de nitrato medidas por cromatografia iônica. Quantidades adsorvidas no equilíbrio (qe, constantes de distribuição (Kd=qe/Ce, fator de separação (R L e variação de energia livre de Gibbs (ΔGº, foram calculados com base nos dados experimentais de adsorção. Os resultados de DRX e FTIR confirmaram a formação do complexo caulinita-uréia a partir do tratamento efetuado no rejeito de caulim. Os seguintes dados foram obtidos nos processos de adsorção de nitrato, no rejeito de caulim natural e tratado com uréia: q max= 18,17 e 14,1 mmol kg-1; Kd=0,7-2,2 e 0,45-1,5 L Kg-1; R L= 0,35-0,85 e 0,25-0,80; ΔGº= -9,3 a -5,8 e -9,1 a -5,4 kJ mol-1, respectivamente. Os valores de Kd, ΔGº e R L são típicos de processos de adsorção física (fisissorção, viáveis e espontâneos e os de q max indicam que a retenção de nitrato em rejeito de caulim natural é mais eficiente do que em rejeito modificado com uréia.

  18. ANKARA İLİ BİRİNCİ LİG TAKIMLARINDA OYNAYAN BAYAN VOLEYBOLCULARIN BAZI FİZİKSEL VE FİZYOLOJİK PARAMETRELERİNİN İNCELENMESİ / A STUDY ON CERTAIN PHYSICAL AND PHYSIOLOGICAL PARAMETERS OF THE FEMALE VOLLEYBALL PLAYERS IN ANKARA VOLLEYBALL FIRST LEAGUE

    OpenAIRE

    Önder, H. Uğut; ELER, Serdar

    2010-01-01

    Bu çalışmanın amacı, Türkiye Vokybol (I. Lıg) Ankara takımlannda voleyboloynayan toplam 56bayan sporculann bazı fiziksel ve fizyolojik profi ıı erini belirlemek, literaliir çerçevesinde durumtespıtlerinin yapılmasıdır.Deneklerden alınan ölçümler sonucunda şu değerlere ulaşllmıştır.Yaşlar sporcularda 24.21 ± 4.83 yıl,"ücut ağırlığı 68 ± 7.74 kg, dikey sıçrama değeri 47.52 ± 3.89 cm, yatay sıçrama değeri 217.33 &...

  19. Analysis of travel motivations of tourists in terms of their demographical characteristics: The case of AnkaraYabancı turistlerin seyahat motivasyonlarının demografik özellikleri açısından analizi: Ankara örneği

    Directory of Open Access Journals (Sweden)

    İrfan Yazıcıoğlu

    2016-08-01

    Full Text Available The fact that tourists have different desires and expectations and those’ve always changed as well, the studies that focus on understanding the components motivate tourists to buy, have been important more and more. This study aims to reveal travel motivation of foreign tourists who visited Ankara city and to determine if tourists’ motivations vary according to demographical characteristics (gender, marial status, income, education, employment, age, nationality. As an implementation area for this study; Ankara is chosen and 390 foreign tourists visiting Ankara are applied. As data collection instrument, survey forms are used within the scope of this study and in analysis of collected data, t test and Anova are used.  As a result of the analyses done; it is determined that their common motivations show a significant difference according to their gender and nationality, but doesn’t show a significant difference according to their marial status, income, education, employment, age. When it is analyzed in terms of dimensions;  it is determined that the motivations of “knowledge” and “relaxation” show a significant difference according to their gender and nationality;  the motivation of “entertainment” shows a significant difference according to their gender, marial status, income, employment, and finally the motivation of “prestige” shows only a significant difference according to their nationality.   Özet Turistlerin farklı istek ve beklentilere sahip olmaları ve bu isteklerin sürekli değişmesi nedeniyle turistleri satın almaya motive eden unsurları anlamaya yönelik çalışmalar önem kazanmıştır. Bu araştırmanın amacı, yabancı turistlerin seyahat motivasyonlarının belirlenmesi ve seyahat motivasyonlarının demografik özelliklere (cinsiyet, medeni durum, gelir, eğitim, çalışma durumu, yaş ve milliyet göre farklılık gösterip göstermediğinin ortaya konmasıdır. Bu amaç do

  20. Influência da concentração de soro de leite e de amido modificado sobre algumas características de composição de bebidas lácteas não fermentadas

    Directory of Open Access Journals (Sweden)

    Shana P. Ferreira

    2009-12-01

    Full Text Available Este trabalho teve como objetivo estudar a influência da concentração de soro de leite (26, 30, 40, 50 e 54% e de amido modificado (0,1; 0,2; 0,4; 0,6 e 0,7% sobre os teores de lactose, sólidos totais e acidez titulável de bebidas lácteas não fermentadas. Foram elaboradas 11 formulações empregando-se o planejamento estatístico Delineamento Composto Central Rotacional (DCCR para avaliar a influência dos fatores estudados, sobre as variáveis avaliadas. Para análise estatística dos resultados realizou-se análise de regressão. Constatou-se que as concentrações de soro avaliadas apresentaram efeito significativo sobre os teores de lactose, sólidos totais e acidez titulável, e o amido modificado não apresentou efeito significativo.

  1. ANKARA HAYMANA TÜRKŞEREFLİ GÖLETİ SULAMA ALANINDAKİ TOPRAKLARIN SULAMA PARAMETRELERİ VE TUZLULUK YÖNÜNDEN İNCELENMESİ

    OpenAIRE

    ANLIATAMER, Eser

    2007-01-01

    Bu çalışmada Devlet Su İşleri (DSİ) tarafından yapılması planlanan Ankara-Haymana-Türkşerefli Göleti sulama alanının yıllar içerisinde değişim gösteren tuzluluk durumu araştırılmıştır. Bu bağlamda 1998 yılı ile 2006 yılı toprak ve su örneklerinde gerekli olan değerleri karşılaştırılmıştır. 8 yıllık süre içinde 49 ha alanın 1. sınıf limitten (EC:2-4 dS/m) 2. sınıf (EC:4-8 dS/m) tuzluluk limitine; 77 ha alanın 1. sınıf limitlerden (EC:2-4 dS/m) 3. Sınıf (EC:8-12 dS/m) limitlere; 221 ha alanın b...

  2. Kesikköprü (Ankara) baraj gölündeki bentik organizma türleri ve mevsimsel değişimleri

    OpenAIRE

    AHISKA, Seyhan

    1999-01-01

       Nisan 1995 - Mayıs 1996 tarihleri arasında Kesiköprü Baraj Gölü (Ankara) 'nde yapılan bu çalışmada, bentik fauna, biyomas değerleri ve mevsimsel değişimler, gölün bazı fiziksel ve kimyasal özellikleri ile birlikte araştırılmıştır. Göldeki bentik faunanın sayısal olarak % 26,88 'ini Chironomidae larvaları, % 32,59' unu Oligochaeta türü ve % 40,5 'ini diğer bentik omurgasız gruplarının oluşturduğu saptanmıştır. Bentik faunanın yaş ağırlığı yıllık orta l...

  3. The Mediating Role of Job Stress on The Effect of Mobbing on Burnout: An Analysis of Social Security Organization Employees in The District of Ankara

    Directory of Open Access Journals (Sweden)

    Yücel Erol

    2015-06-01

    Full Text Available In this study, due to the influence of Mobbing (MO on Burnout (BU, the mediating role of Job Stress (JS on employees is examined. Hence, 826 employees of public and private institutions in Ankara were included in the sample. The data obtained by a questionnaire that prepared by Leymann (1996’s MO scale, and Maslach (1981's BU scale and Haynes (1996's JS scale. Data was evaluated by using One-Way ANOVA, correlation, regression, and multiple regression SPSS. As can be seen from the results of research, applied and perceived MO has a significant impact on employees' JS’s levels and becomes effective in the creation of JS. On the other hand, BU syndrome were seen as a consequence of the resulting JS. These conditions affect each other and existence of one within the organizational structure, results emergence of the other two. Therefore, management of organization, all levels of managers, and employees as individuals should be aware of these psychological states and conduct studies aimed for its prevention.In this research, meaningful relationships have been identified in the mediating role of JS at the impact of MO on BU. On the other hand in terms of demographic variables, significant variations were found in the levels of MO, BU, and JS

  4. FAMILY-WORK CONFLICT AND LIFE SATISFACTION AMONG WORKERS OF ANKARA YOUTH SERVICES AND PROVINCIAL DIRECTORATE OF SPORTS ACCORDING TO DIFFERENT VARIABLES

    Directory of Open Access Journals (Sweden)

    Mustafa Önder

    2016-04-01

    Full Text Available In this study Work-Family Conflict, Family-Work Conflict and Life Satisfaction were carried out to indicate 329 staff’s relationship between each other, who work in Ankara Youth Services and Sports Province Management, by determining whether work-family conflict, family-work conflict and life satisfaction differientiate or not according to socio-demographic variables. According to obtained results, it was concluded that gender, age, having management position, number of children and duration of experience in job were not significant on family-work conflict, work-family conflict and life satisfaction. On the other hand married staff compared to single ones and staff whose spouse works, experienced the work- family conflict more. Moreover, it was deduced that education level and income level were significant on life satisfaction. Furthermore, a positive way medium level relationship between family- work conflict and work- family conflict was found out supporting the obtained data that staff who has high level of family- work conflict will have relationship between life satisfaction and between family- work conflict / work- family conflict was statistically meaningless.

  5. One-prime multi-boost strategy immunization with recombinant DNA, adenovirus, and MVA vector vaccines expressing HPV16 L1 induces potent, sustained, and specific immune response in mice.

    Science.gov (United States)

    Li, Li-Li; Wang, He-Rong; Zhou, Zhi-Yi; Luo, Jing; Xiao, Xiang-Qian; Wang, Xiao-Li; Li, Jin-Tao; Zhou, Yu-Bai; Zeng, Yi

    2016-04-01

    Human papillomavirus (HPV) is associated with various human diseases, including cancer, and developing vaccines is a cost-efficient strategy to prevent HPV-related disease. The major capsid protein L1, which an increasing number of studies have confirmed is typically expressed early in infection, is a promising antigen for such a vaccine, although the E6 and E7 proteins have been characterized more extensively. Thus, the L1 gene from HPV16 was inserted into a recombinant vector, AdHu5, and MVA viral vectors, and administered by prime-boost immunization. Virus-like particles were used as control antigens. Our results indicate that prime-boost immunization with heterologous vaccines induced robust and sustained cellular and humoral response specific to HPV16 L1. In particular, sera obtained from mice immunized with DNA + DNA + Ad + MVA had excellent antitumor activity in vivo. However, the data also confirm that virus-like particles can only elicit low levels cellular immunity and not be long-lasting, and are therefore unsuitable for treatment of existing HPV infections. PMID:26821205

  6. Estudo de misturas asfálticas com ligante modificado pelo polímero EVA para pavimentos urbanos de Manaus - AM Study of asphalt mixtures containing a binder modified with EVA polymer for use in urban pavements in Manaus

    Directory of Open Access Journals (Sweden)

    Daniela M. G. D'Antona

    2011-01-01

    Full Text Available A presente pesquisa buscou soluções com materiais alternativos visando à construção de pavimentos urbanos para Manaus (AM com maior vida útil e condizente com as suas condições de serviço, em particular, o seu desempenho mecânico frente às temperaturas regionais. Estudou-se o ligante regional, CAP 50/70, misturado com 4% do polímero EVA, e como partícipe em compósitos asfálticos. A incorporação do mencionado polímero teve como objetivo melhorar suas propriedades reológicas e, por conseguinte, o comportamento mecânico desse compósito - concreto asfáltico, empregado como revestimento nas vias urbanas da Capital do Estado do Amazonas. Os ligantes (virgem e modificado foram caracterizados conforme a especificação da ANP e a metodologia Superpave. As misturas asfálticas foram avaliadas pelo ensaio de fluência por compressão axial, com carregamento dinâmico à temperatura de 40 ºC. O material asfáltico modificado com EVA (AMP EVA demonstrou melhores propriedades reológicas ou equivalentes ao asfalto convencional (REMAN, ao longo de todos os ensaios de caracterização. As misturas alternativas compostas com o CAP modificado (SEVA obtiveram desempenho mecânico superior ao de misturas com cimento asfáltico habitualmente comercializado na região (SMAN.In this research we investigated alternative materials to build urban pavements in Manaus, seeking for pavements with longer lifetime for usage and suitable mechanical properties to withstand the local temperatures. The asphaltic mixtures contained a regional binder, namely CAP 50/70, mixed with 4% of EVA polymer. The incorporation of the latter polymer was aimed at improving the rheological properties of asphaltic concrete used in roads in the Amazonas' capital. The asphalt binders (original and modified were characterized in accordance with the ANP (National Petroleum Agency specification and the Superpave methodology. The asphalt mixtures had their mechanical properties

  7. Orthodontic-logaoedic treatment using a modified Hawley device. Tratamiento ortodóncico – logopédico utilizando un aparato de Hawley modificado.

    Directory of Open Access Journals (Sweden)

    Yordanis Boza Mejias

    . Objetivo : Comprobar la utilidad del aparato de Hawley modificado en pacientes con maloclusiones dentarias producidas por hábitos deformantes y portadores de dislalias. Métodos : Estudio observacional, analítico, comparativo, realizado en la escuela primaria ¨Guerrillero Heroico¨ del municipio de Cienfuegos. Se tomó una muestra no probabilística de 32 pacientes a los cuales se les realizaron dos mediciones. Se estudiaron las siguientes variables: Número de historia clínica, expediente, anomalías dentomaxilofaciales, hábitos como succión digital, deglusión atípica, análisis de la pronunciación y niveles de articulación. Se colocó Hawley con perforación en forma ovalada, a nivel de las rugosidades palatinas, lo que cambia la forma tradicional de la rejilla. Resultados : Prevaleció la maloclusión de Clase I. Las anomalías dentomaxilofaciales más frecuentes fueron la vestíbuloversión ( 87,5 %, resalte aumentado (84,4 % y el cierre bilabial anormal (68,8 %, las dislalias se redujeron durante el tratamiento de 81,2 % al comienzo hasta 28, 1 % al final de este.

  8. Evaluation of DNA Damage in Common Carp (Cyprinus carpio L. by Comet Assay for Determination of Possible Pollution in Lake Mogan (Ankara

    Directory of Open Access Journals (Sweden)

    İsmet Çok

    2011-01-01

    Full Text Available Contamination of the aquatic environment with various concentrations of pollutants results in unexpected threats to humans and wildlife. The consequences of exposure and metabolism of pollutants/xenobiotics, especially carcinogens and mutagens, can be suitably assessed by investigating severe events, such as DNA damage; for example, DNA adducts and DNA strand breaks. One of the commonly used techniques to detect DNA damage in aquatic organisms is single-cell gel electrophoresis (comet assay. This study was carried out using Cyprinus carpio in order to identify the possible pollution in Lake Mogan, near Ankara, Turkey, where the city's sewer system and pesticides used in agriculture are believed to be the common causes of pollution. From the comet assay, the tail length (μm, tail intensity (%, and tail moment values of fish caught from Lake Mogan were found to be 31.10 ± 10.39, 7.77 ± 4.51, 1.50 ± 1.48, respectively, whereas for clean reference sites they were found to be 22.80 ± 1.08, 3.47 ± 1.59, 0.40 ± 0.51, respectively. The values are statistically different from each other (p < 0.0001, p < 0.0001, and p < 0.0013, respectively. These results indicate that Lake Mogan may be polluted with substances that have genotoxic effects and constitute an early warning for the lake system. Further detailed research is needed to establish the source of the pollution and the chemicals responsible.

  9. An evaluation of the attributes of a political product (political leader by young voters: A research on the students at the universities in Ankara

    Directory of Open Access Journals (Sweden)

    Cihat Polat

    2008-01-01

    Full Text Available In democratic countries, the way of a political party to get in depends on convincing adequate number of voters to politically buy its political product package presented in political market during the election campaign. Political leader is assumed to be one of the most important –even the most important- components of the political product package almost in all over the world. Thence, political leader has a considerable significance in the entire political package of a party and the characteristics of him/her are the primary determinant of the quality of it.Especially in the markets as in Turkey that votes mostly tend to cluster considering the characteristics of the political leader, the mentioned characteristics play an important role in voting decision process. Though the proportion of university students to total number of voters in Turkish political market is not so much, the market is mostly composed of young voters. Nonetheless, as university students compose of the highest-educated proportion of total voters, they relatively have great potential as a pressure group in respect of affecting political decisions and being an opinion of policy. In consequence, it may be assumed that this group has a specific political weight. To be aware of the characteristics that university students seek in a political leader is a topic of interest in both political practices and political marketing theory. This study presents the results of a survey conducted on a sample of 500 students educating in different universities in Ankara. The survey aims to find out the desired characteristics of a political leader in the eyes of university students and to rank them in a known set according to their importance levels. The findings of the study seem to be interesting in scope of both political parties and field of political marketing.

  10. Environmental Harmony and Evaluation of Advertisement Billboards with Digital Photogrammetry Technique and GIS Capabilities: A Case Study in the City of Ankara

    Science.gov (United States)

    Aydın, Cevdet C.; Nişancı, Recep

    2008-01-01

    Geographical Information Systems (GIS) have been gaining a growing interest in Turkey. Many local governments and public agencies have been struggling to set up such systems to serve the needs and meet public requirements. Urban life shelters the advertisement reality which is presented at various places, on vehicles, shops etc. in daily life. It can be said that advertisement is a part of daily life in urban area, especially in city centers. In addition, one of the main sources of revenue for municipalities comes from advertising and notices. The advertising sector provides a great level of income today. Therefore advertising is individually very important for local governments and urban management. Although it is valuable for local governments, it is also very important for urban management to place these advertisement signs and billboards in an orderly fashion which is pleasing to the eye. Another point related to this subject is the systematic control mechanism which is necessary for collecting taxes regularly and updating. In this paper, first practical meaning of notice and advertisement subject, problem definition and objectives are described and then legal support and daily practice are revised. Current practice and problems are mentioned. Possibilities of measuring and obtaining necessary information by using digital images and transferring them to spatial databases are studied. By this study, a modern approach was developed for urban management and municipalities by using information technology which is an alternative to current application. Criteria which provide environmental harmony such as urban beauty, colour, compatibility and safety were also evaluated. It was finally concluded that measuring commercial signs and keeping environmental harmony under control for urban beauty can be provided by Digital Photogrammetry (DP) technique and GIS capabilities which were studied with pilot applications in the city center of Ankara.

  11. Environmental Harmony and Evaluation of Advertisement Billboards with Digital Photogrammetry Technique and GIS Capabilities: A Case Study in the City of Ankara

    Directory of Open Access Journals (Sweden)

    Recep Nisancı

    2008-05-01

    Full Text Available Geographical Information Systems (GIS have been gaining a growing interest in Turkey. Many local governments and public agencies have been struggling to set up such systems to serve the needs and meet public requirements. Urban life shelters the advertisement reality which is presented at various places, on vehicles, shops etc. in daily life. It can be said that advertisement is a part of daily life in urban area, especially in city centers. In addition, one of the main sources of revenue for municipalities comes from advertising and notices. The advertising sector provides a great level of income today. Therefore advertising is individually very important for local governments and urban management. Although it is valuable for local governments, it is also very important for urban management to place these advertisement signs and billboards in an orderly fashion which is pleasing to the eye. Another point related to this subject is the systematic control mechanism which is necessary for collecting taxes regularly and updating. In this paper, first practical meaning of notice and advertisement subject, problem definition and objectives are described and then legal support and daily practice are revised. Current practice and problems are mentioned. Possibilities of measuring and obtaining necessary information by using digital images and transferring them to spatial databases are studied. By this study, a modern approach was developed for urban management and municipalities by using information technology which is an alternative to current application. Criteria which provide environmental harmony such as urban beauty, colour, compatibility and safety were also evaluated. It was finally concluded that measuring commercial signs and keeping environmental harmony under control for urban beauty can be provided by Digital Photogrammetry (DP technique and GIS capabilities which were studied with pilot applications in the city center of Ankara.

  12. Método sol-gel modificado para obtenção de alumina nanoencapsulada com terras raras Sol-gel modified method for obtaining alpha-alumina nanocoated with rare earth

    OpenAIRE

    Maciel, A. P.; E. R. Leite; Longo, E.; J. A. Varela

    2005-01-01

    Neste trabalho, alfa-alumina foi nanoencapsulada com óxido de lantânio usando um método sol-gel modificado. Um precursor polímero de lantânio foi usado para encapsular a alfa-Al2O3, dispersa em água. A influência da nanocápsula de óxido de lantânio na sinterização e microestrutura da alfa-alumina foi investigada usando-se técnicas de microscopia eletrônica. A microscopia eletrônica de varredura dos grãos nanoencapsulados revelou que compostos de lantânio precipitam no contorno de grão da alfa...

  13. Construção e avaliação de eletrodos modificados com hexacianoferrato de prata para a determinação amperométrica de isoniazida

    OpenAIRE

    Oliveira, Paulo Roberto

    2013-01-01

    Resumo: Um método rápido e simples para a determinação de isoniazida foi desenvolvido com um sistema de análise por injeção em fluxo (FIA) usando um eletrodo impresso (EI) de carbono modificado com hexacianoferrato de prata (Ag-HCF) como detector amperométrico. A isoniazida (INZ) é um agente anti tuberculástico frequentemente utilizado para fins clínicos. Devido aos acidentes de intoxicação levarem a morte, a determinação de níveis deste fármaco em fluidos do corpo humano é vital para verific...

  14. Uso de fluorescência em um método de dissector modificado para estimar o número de miócitos no tecido cardíaco

    OpenAIRE

    2012-01-01

    FUNDAMENTO: Métodos convencionais de dissector atualmente requerem consideráveis custos financeiros, técnicos e operacionais para estimar o número de células, incluindo cardiomiócitos, em uma área de 3D. OBJETIVO: Usar a microscopia de fluorescência em um método de dissector modificado para determinar o número de miócitos no tecido cardíaco em condições normais e patológicas. MÉTODOS: O estudo empregou camundongos Wistar machos com quatro meses de idade e peso de 366,25 ± 88,21 g randomizados...

  15. Estudio del equilibrio y cinética de adsorción de Cd(II), Ni(II) y Cr(VI) usando Quitosano y Quitosano modificado con cobre

    OpenAIRE

    Benavente, M.; Castro, S; N. Betanco; F. Canelo; López, X. (Xosé); García, A.

    2013-01-01

    En este trabajo, se estudió el equilibrio y la cinética de adsorción de Cd(II), Ni(II) en quitosano y Cr(VI) en quitosano modificado con cobre. Para ello, soluciones de iones metálicos a diferentes concentraciones, fueron puestas en contacto con el adsorbente y se agitaron por un período de 3 – 4 h. Al final del proceso, las muestras fueron analizadas en un espectrómetro de absorción atómica. Los datos experimentales del equilibrio de adsorción de los iones metálicos fueron evaluados aplicand...

  16. Obtenção e caracterização de blendas de asfalto CAP 20, modificado com poliestireno reciclado, resíduos de pneu e lignina organossolve

    Directory of Open Access Journals (Sweden)

    Vagner Roberto Botaro

    2006-03-01

    Full Text Available O presente trabalho trata da obtenção e caracterização de blendas envolvendo matrizes asfálticas (CAP 20, em misturas com poliestireno reciclável (PS, pó de pneu (PPN e lignina organossolve (LIG. Quando se adicionam polímeros à matriz asfáltica, pretende-se, principalmente, aumentar a impermeabilidade, impedindo a deterioração pela água, diminuir a fragilidade a baixas temperaturas e diminuir o desgaste por abrasão. As propriedades físicas, incluindo índice de penetração, ponto de amolecimento e índice de susceptibilidade térmica do CAP 20 modificado, foram analisadas e comparadas com o CAP 20 não modificado. Os resultados obtidos mostram que a adição de PPN, PS e LIG afeta as propriedades mecânicas das blendas formadas com o CAP 20, diminuindo os índices de penetração e aumentando os pontos de amolecimento de uma maneira geral.The present work describes the production and the characterization of blends involving mixtures of CAP 20 with recycled polystyrene, tire powder or organosolve lignin. The main objective related with the modification of the asphalt matrixes is to increase the impermeability impeding water deterioration, to reduce the fragility for low temperatures and to reduce the abrasion phenomena. The physical properties, including penetration index, softening point and index of thermal susceptibility were analyzed and then compared with CAP 20 matrix. The main results show that the addition of tire powder, polystyrene and lignin affect the mechanical properties of the blends when compared with CAP 20, showing a general decrease of penetration and a general improvement of the softening point after addition of modifiers.

  17. La relación entre los riesgos, la precaución y la responsabilidad en los daños al medio ambiente por la liberación de organismos genéticamente modificados.

    Directory of Open Access Journals (Sweden)

    Sebastián Rebolledo Aguirre

    2012-05-01

    Full Text Available ResumenEl presente trabajo tiene por objeto mostrar la relación teórica y práctica entre la compleja noción de los riesgos; el principio precautorio como norma jurídico-ambiental, y la institución fundamental en todo sistema jurídico de la responsabilidad por daños.de los daños al medio ambiente por la liberación de organismos genéticamente modificados es una utilísima herramienta para determinar el régimen de responsabilidad aplicable, la interpretación de las normas jurídicas involucradas y controlar la discrecionalidad administrativa.Se explica que los riesgos inciertos son el fundamento y la razón que dan operatividad al principio precautorio, y que la funcionalidad de éste en el contexto de los daños al medio ambiente por la liberación de organismos genéticamente modificados es una utilísima herramienta para determinar el régimen de responsabilidad aplicable, la interpretación de las normas jurídicas involucradas y controlar la discrecionalidad administrativa.AbstractThis paper aims to show the theoretical and practical relationship between the complex notion of risk, the precautionary principle as an environmental legal standard, and the legal doctrine of liability for damages.It explains that uncertain risks are the foundation and the reason that makes the precautionary principle operative, and that, in the context of environmental damages caused by the release of GMOs, it is a very useful tool to determine the liability regime and the interpretation of the rules involved, and to control administrative discretion.

  18. Rotulagem de alimentos que contém Organismos Geneticamente Modificados: políticas internacionais e Legislação no Brasil Labeling of food containing Genetically Modified Organisms: international policies and Brazilian legislation

    Directory of Open Access Journals (Sweden)

    Thadeu Estevam Moreira Maramaldo Costa

    2011-08-01

    Full Text Available O crescimento da área de superfície plantada com as culturas geneticamente modificadas, com a consequente liberação dessas lavouras para o ambiente e para a comercialização, levantou questionamentos sobre a segurança destes produtos. A entrada em vigor do Protocolo de Cartagena sobre Biossegurança , fez com que houvesse a necessidade de aquisição de informações e capacitação nesta área para a implementação de políticas de biossegurança e para tomadas de decisões por partes dos governos em níveis nacionais, regionais e internacionais. O presente artigo apresenta as duas principais vertentes políticas sobre rotulagem de produtos geneticamente modificados (uma adotada pelos Estados Unidos da América e outra pela União Europeia, assim como a posição adotada pelo Brasil e sua atual legislação acerca de rotulagem e liberação comercial de produtos geneticamente modificados (GM.The increase in surface area planted with genetically modified crops, with the subsequent transfer of such crops into the general environment for commercial trade, has raised questions about the safety of these products. The introduction of the Cartagena Protocol on Biosafety has led to the need to produce information and ensure training in this area for the implementation of policies on biosafety and for decision-making on the part of governments at the national, regional and international level. This article presents two main standpoints regarding the labeling of GM products (one adopted by the United States and the other by the European Union, as well as the position adopted by Brazil and its current legislation on labeling and commercial release of genetically modified (GM products.

  19. Características físicas de filmes biodegradáveis produzidos a partir de amidos modificados de mandioca Physical characteristics of cassava modified starch films

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    Celina Maria Henrique

    2008-03-01

    Full Text Available Amidos de mandioca podem ser matérias-primas para a obtenção de filmes biodegradáveis, sendo que para a formação destes é necessária a elaboração de suspensões filmogênicas. Alguns processos de modificação do amido podem torná-lo miscível em água fria, e outros processos de modificação podem alterar as propriedades dos filmes, tornando-os mais fortes e flexíveis. O objetivo deste trabalho foi verificar as características físicas de filmes biodegradáveis elaborados com amidos modificados de mandioca pelo processo de casting (desidratação de uma solução filmogênica sobre placas de Petri. Os amidos modificados utilizados foram: cross linked; carboximetilamido (CMA de baixa viscosidade e alta viscosidade e esterificado. A viscosidade é fator importante para a elaboração da suspensão filmogênica e foi avaliada utilizando-se o equipamento Rapid Visco Analyser (RVA. Os filmes elaborados foram comparados a um filme de PVC comercial com espessura de 0,0208 a 0,0217 mm. Os amidos foram caracterizados por avaliação da composição físico-química, granulometria, microscopia eletrônica e viscosidade (Rapid Visco Analyser. A análise por microscopia eletrônica dos filmes ressaltou as diferenças entre os diferentes amidos utilizados. O RVA mostrou que, com exceção do cross linked, todos os amidos modificados apresentaram certa solubilidade a frio, o que facilita o preparo das soluções filmogênicas, entretanto, todos os amidos modificados apresentaram redução acentuada da tendência à retrogradação, propriedade geralmente associada à formação de filmes. As espessuras dos filmes de amido variaram de 0,0551 a 0,1279 mm, cujas espessuras mínimas foram a dos filmes de amido cross linked. Os filmes mostraram-se transparentes, manuseáveis e bem homogêneos. Não houve interferência da espessura na permeabilidade ao vapor d'água, e os filmes com 5% de matéria-seca, independente do tipo de amido modificado

  20. Uso de fluorescência em um método de dissector modificado para estimar o número de miócitos no tecido cardíaco Uso de fluorescencia en un método de disector modificado para estimar el número de miocitos en el tejido cardíaco Use of fluorescence in a modified disector method to estimate the number of myocytes in cardiac tissue

    OpenAIRE

    Rômulo Dias Novaes; Arlete Rita Penitente; André Talvani; Antônio José Natali; Clóvis Andrade Neves; Izabel Regina Santos Costa Maldonado

    2012-01-01

    FUNDAMENTO: Métodos convencionais de dissector atualmente requerem consideráveis custos financeiros, técnicos e operacionais para estimar o número de células, incluindo cardiomiócitos, em uma área de 3D. OBJETIVO: Usar a microscopia de fluorescência em um método de dissector modificado para determinar o número de miócitos no tecido cardíaco em condições normais e patológicas. MÉTODOS: O estudo empregou camundongos Wistar machos com quatro meses de idade e peso de 366,25 ± 88,21 g randomizados...

  1. The use of certified seeds of improved wheat varieties in farms and the contributions of certified seed usage to enterprise economies: the case of Ankara province in Turkey.

    Science.gov (United States)

    Tanrivermiş, Harun; Akdoğan, Ilyas

    2007-12-15

    In this research, the use of certificated seeds of improved wheat varieties in farms of Ankara, which have an important share in wheat production of Turkey and the contributions of the certified seeds usage to enterprise economy have been evaluated by using data collected from farms via a questionnaire. The low level of use of certificated wheat seed in farms is associated with many factors, such as price of the seed (cost), yield gains and suitability of the varieties for the farming aims of producers. Average productivity is 49.5% and average net profit is 39.7% higher in the production of wheat with certificated seeds of improved varieties in farms in comparison to farming with traditional varieties. Average productivity in wheat production with uncertified seeds of improved varieties is 24.9% and net profit per unit area is 24.3% higher than wheat production with traditional varieties. The net economic benefit of wheat production using certificated seeds of improved varieties and that of wheat farming with uncertified seeds has been estimated at $ 102.40 and $ 62.70 ha(-1), respectively. Some $ 6.4 benefit is generated in return for $ 1 of expense in wheat farming with certificated seeds of improved varieties instead of traditional varieties. In the absence of state subsidies for certificated seeds the use of uncertified seeds seems to be advantageous for producers. While the amount of subsidy per hectare will be a minimum of $ 101 for certificated seeds per hectare of planting area (as technological improvement), the support will be encouraging for usage of certified seeds. Many variables, such as farm size, use of certified seeds, topography of land, production system, education level and frequency of visiting the agriculture organizations of the producers influence the level of gross profit per unit area in wheat farming. Low level of use of certificated seed in farms decreases the economic benefit of new varieties which are developed as a result of long

  2. İngiliz Kültür Heyeti Ankara Kütüphanesi Okuyucuları ve Çevrimiçi Okuyucu Katalogu

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    Selma Alpay Aslan

    1993-03-01

    Full Text Available Çevrimiçi okuyucu kataloglarının (ÇOK, derme ile ilgili kaynakçasal bilgilere erişim olanaklarını zenginleştirme, tümleşik dizgelerde ödünç verme bilgisinini de içerme gibi yararları vardır. İngiliz Kültür Heyeti Ankara Kütüphanesi (İKHAK 1992'de otomasyona geçmiş ve katalogunu ÇOK'a dönüştürmüştür. Bir okuyucu anketine dayanılarak okuyucuların bu yeniliği nasıl karşıladıkları, ÇOK'u ne ölçüde değerlendirdikleri, nasıl öğrendikleri ve kullandıkları, karşılaştıkları güçlüklerin neler olduğu ve ÇOK hakkındaki düşünceleri araştırılmıştır. Araştırma, İKHAK okuyucularının %64.4'nün katalog türü olarak ÇOK'u yeğlediklerini, kataloğu kullananların %75.3'nün ÇOK'un kullanımını kolay bulduğunu, bu kullanıcıların yaklaşık yarısının kullanımı doğrudan dizgiden öğrenmek istediklerini ve doyum düzeyinin %80'in üzerinde olduğunu göstermiştir. Yorum yapan okuyucular, yukarıda belirtilen yararlar dışında, ÇOK'un okuyucuya zaman kazandırdığım, kitap seçimini kolaylaştırdığını da vurgulamışlardır. Bütün bu bulgular okuyuculara ÇOK sunabilmenin hizmetlerin geliştirilmesinde yararlı bir uygulama olduğunu ve bu yatırımın yerindeliğini ortaya koymuştur.

  3. Political And Economic Approach Of Women Working In Executive Levels In The Service Sector: Ankara State Opera And Ballet (Asob Case

    Directory of Open Access Journals (Sweden)

    Ilkben Akansel

    2012-04-01

    Full Text Available Political economy is related to how capitalist system works by means of distribution of economical and cultural sources which has also a connection between the circumstances of women’s working life. Because hegemony, which is reflected to hegemony’s ideology, constitutes its force by the mediation of the concepts: ‘discrimination’ and ‘segregation’. This force sometimes can effect women as both earning much less in some labor-force market, facing to harassment etc. and being obstructed to get top-level managerial positions in which institutes their work by means of political economy point of view.In this study, we will examine the art sector which is one of the sub-branches of service sector in the labor-force market. Our case is Ankara State Opera and Ballet (ASOB. In this case, we aim to display how the institution is founded, and accepted as a part of modernization with the beginning of Republic of Turkey and changed especially by means of political approaches in the last years.While doing this, we will elaborate the concepts of ‘discrimination’ and ‘segregation’ which issues women face in the labor-force market. Then, we will relate these three concepts with Antonio Gramsci’s ‘hegemony’ and Louis Althusser’s ‘ideology’, Michel Foucault’s ‘power’  and we will examine how women are assigned to the executive positions in the case of institution which we have chosen.We will study the circumstances which may be narrowed as; whether women are permitted to work in the top-level management or not, how many women have been worked in the top-level management in the history of the institution, if quantity has a meaning or not etc., by means of political economy.By the mediation of aforementioned subjects, we will analyse the reasons of women being prevented to work in the top-level management in the working life by economical, political and social aspects. Consequently, we will elaborate how political economy, in

  4. The Use of Alternate Ligno-cellulosic Raw Materials Banana (Musa sapientum Ankara (Calotropis procera and Pineapple (Ananas comosus in Handmade Paper & their Blending with Waste Paper.

    Directory of Open Access Journals (Sweden)

    Atul Kumar

    2013-09-01

    Full Text Available The studies were made to established suitability of lingo-cellulosic raw materials namely leaf fibreBanana (Musa Sapientum, bast fibre Ankara (Calotropis Procera, & leaf fibre Pineapple (Ananas Comosus for making pulps for handmade paper industry. This should help in providing a cost effective, good quality cellulosic raw material as an alternate to cost prohibitive traditionally used cotton hosiery waste traditionally used for manufacturing good quality handmade paper & it’s products. This will help in improving the cost economics & competitiveness of the Indian paper industry in the global market besides addressing the problems of environment & global warming. The aim of the research was to study the extraction, morphology, chemical composition and pulping of these fibres and relate these properties to the composite properties obtained with these fibres as reinforcement with short fibres viz. waste paper. For the pulp production to be feasible it is essential to use suitable pulping methods, which maximize the yield of pulp and introduce as low damage as possible to the fibres. The different pulping methods were applied to these fibre to get optimized strength properties papers. The Studies thus carried out provide useful information about the nature of these raw materials, suitable pulping & bleaching process to produce an eco-friendly handmade paper and converted products. The research work provides a good quality cost effective lingo-cellulosic raw material for handmade paper industries with a possibility of replacement of the expensive and traditionally used cotton hosiery waste.The paper thus produced using environmental friendly pulping and bleaching process is characterized for its strength properties like tensile, tear, bursting, folding endurance and other parameters. The effluents generated from pulping and bleaching of above ligno-cellulosic waste materials were characterized for various pollution parameters like Residual Alkali

  5. Ankara Üniversitesi öğrencilerinin kadınların spor etkinliklerine katılımlarına ilişkin görüşleri

    OpenAIRE

    KIZILYALLI, Meltem

    2014-01-01

    Bu araştırmanın amacı Ankara Üniversitesi öğrencilerinin kadınların spor etkinliklerine katılımlarına ilişkin görüşlerini belirlemektir. Çalışmanın evrenini, 2012-2013 akademik yılında Ankara Üniversitesinde öğrenim gören İletişim Fakültesi, İlahiyat Fakültesi ve Mühendislik Fakültesi Yabancı Diller Hazırlık ve 4. Sınıf öğrencileri oluşturmaktadır. Bu amaçla üç fakülteden 2397 kişilik evrenden, 750 kişiye ölçme aracı uygulanmıştır. Araştırmanın verileri araştırmacı tarafı...

  6. Sanat sektöründe kadin işgücünün konumu: Ankara Devlet Opera ve Balesi çerçevesinde bir örnekleme

    OpenAIRE

    Akansel, İlkben

    2004-01-01

    Sanat sektörü, hizmetler sektörü içinde daha marjinal bir alandır. Sanat sektörü, çok geniş bir alanı kapsadığı için, çalışmamız Ankara Devlet Opera ve Balesi üzerinde temellendirilmiştir. Bu sektörde çalışan kadınlar bir dizi sorunla karşı karşıyadır. Şöyle ki, çalışma yaşamında kadınların genelde karşılaştığı sorunların yanı sıra, kurumdaki kendine özgü çalışma koşulları kadınlar üzerinde ek baskılara neden olabilmektedir. Ankara Devlet Opera ve Balesi’nde yaptığımız ön inceleme ve dah...

  7. Ankara Üniversitesi Uzaktan Eğitim Programına Katılan Öğrencilerin Akademik Başarılarını Yordayan Faktörler

    Directory of Open Access Journals (Sweden)

    Selma Şenel

    2015-12-01

    Full Text Available The aim of this study was to determine the factors predicting academic achievement of Ankara University Distance Education students. The study group of the research consists of 302 first grade students from Ankara University Distance Education Center (ANKUZEM 2010-2011 associate degree programs. Data were formed in four groups respectively “individual characteristics”, “characteristics related with family and working environment”, “characteristics related with usage of information and communication technologies” and “characteristics related with education”. Data were analyzed by stepwise multiple regression analysis. According to the results, individual characteristics as “age”, “working in a full time job” and “being divorced” were found as predictors of students’ academic achievement. Additionally, characteristics related with family and working place as “number of family members living with”, “mothers’ full time working” and “mothers’ graduation type” were other variables predicting academic achievement of students. The characteristics related with education; graduation from “college” and “vocational high school”, “studying with different methods“, “choosing distance education as a university degree”, “studying for getting a degree” are also predictors of academic achievement of students. The characteristics of students related with usage of information and communication technologies were not predictors of academic achievement of students.

  8. La distribución geográfica de la tángara azul-gris (Thraupis episcopus) en hábitats modificados antropogénicamente en México The geographical distribution of the Blue-gray Tanager (Thraupis episcopus) through anthropogenically modified habitats in Mexico

    OpenAIRE

    Erick Rubén Rodríguez-Ruíz; Héctor Arturo Garza-Torres; RÍOS-MUÑOZ, César A.; Navarro-Sigüenza, Adolfo G.

    2011-01-01

    Mediante trabajo de campo, la revisión de bases de datos biológicos e información de la distribución geográfica histórica y actual de Thraupis episcopus se establecieron nuevos registros de anidación en el noreste de México y su ampliación hacia hábitats modificados por actividades humanas en la península de Yucatán y el noreste de México. Además, se evaluó si existía preferencia de la especie por hábitats conservados vs. modificados, por medio de un análisis de escenarios de cambio de uso de...

  9. Hydrolyzed collagen, modified starch and guar gum addition in turkey ham Adição de colágeno hidrolisado, amido modificado e goma guar em presunto de peru

    Directory of Open Access Journals (Sweden)

    Rosa Cristina Prestes

    2012-07-01

    Full Text Available The main problems observed in cooked hams are bad sliceability and excessive fluid loss after cooking. To reduce these problems the industry uses non-meat ingredients such as soy protein and carrageenan, but under Brazilian law, it is not allowed to add starch or modified starch in hams. Three ingredients were tested in the present research: modified starch (0 to 2.0%, gum guar (0 to 0.30% and hydrolyzed collagen (0 to 2.0%, following a 2³ full factorial design with five repetitions in the central point. The guar gum produced low resistance to reheating, however in losses by cooling, the results were adequate. The hydrolyzed collagen tested did not give satisfactory results, showing low acceptance due to formation of gel in the ham and high losses. The developed products were not different from the commercial product in relation to texture (P>0.05. The formulation F6 (2.0% of modified starch was the one with the best results and greater acceptance as detected by the tasters. Based on the results obtained it seems appropriate to propose the legal permission of starch in ham or the creation of a new class of product in which starch addition would be allowed.Os principais problemas observados em presuntos são a má fatiabilidade e a perda excessiva de líquidos após o cozimento. Para reduzir esses problemas, a indústria utiliza ingredientes não cárneos, tais como a proteína de soja e a carragena, porém, segundo a legislação brasileira, não é permitida a adição de amido ou amido modificado. Neste trabalho, foram testados três ingredientes, em presunto cozido de peru: amido modificado (0 a 2,0%, goma guar (0 a 0,30% e colágeno hidrolisado (0 a 2,0%, seguindo um delineamento fatorial completo 2³ com cinco repetições no ponto central. A goma guar apresentou baixa resistência ao reaquecimento, no entanto, nas perdas por resfriamento, os resultados foram adequados. O colágeno hidrolisado testado não proporcionou resultados satisfat

  10. Retention of oral microorganisms on conventional and resin-modified glass-ionomer cements Retenção de microrganismos bucais em cimentos de ionômero de vidro convencionais e modificados por resina

    Directory of Open Access Journals (Sweden)

    Denise PEDRINI

    2001-09-01

    Full Text Available Secondary caries are a worldwide public and socioeconomic problem. The placement of restorations can lead to the development of environmental conditions favorable to microbial colonization, especially on the tooth/restoration interface, which is a predisposing factor for secondary caries. The aim of this study was to evaluate microbial retention on conventional (Chelon-Fil and Vidrion R and resin-modified (Vitremer and Fuji II LC glass-ionomer cements, in situ, using a hybrid composite resin (Z100 as a control. Twelve volunteers wore Hawley appliances with specimens made of all tested filling materials for 7 days. The specimens were then removed from the appliances and transferred to tubes containing 2.0 ml of Ringer-PRAS. Microorganisms from the samples were inoculated onto blood agar and Mitis Salivarius Bacitracin agar and incubated under anaerobiosis (90% N2, 10% CO2, at 37°C, for 10 and 2 days, respectively. The resin-modified glass-ionomer cements and the composite resin retained the same levels of microorganisms on their surfaces. The resin-modified glass-ionomers retained less mutans streptococci than the composite resin and conventional glass-ionomer cements. The conventional glass-ionomer cements retained less mutans streptococci than the composite resin, but that difference was not statistically significant.A cárie secundária representa problema de saúde pública e socioeconômico no mundo. A restauração de dentes acometidos por cárie pode criar condições favoráveis à proliferação microbiana na superfície do material restaurador ou na interface dente/restauração, criando ambiente propício para o estabelecimento de cárie secundária. O objetivo deste estudo foi avaliar a capacidade de retenção de placa bacteriana em cimentos de ionômero de vidro convencionais (Chelon-Fil e Vidrion R e modificados por resina (Vitremer e Fuji II LC e de resina composta híbrida (Z100, utilizada como controle. Nos testes de reten

  11. Impacto do esvaziamento cervical radical modificado no número de linfonodos recuperados, recorrência e sobrevida Impact of modified radical neck dissections on the number of retrieved nodes, recurrence and survival

    Directory of Open Access Journals (Sweden)

    Hugo Fontan Kohler

    2010-06-01

    Full Text Available O esvaziamento cervical é parte integral dos tumores de cabeça e pescoço. Ao longo de sua história, passou por mudanças procurando a melhora do resultado funcional sem perda da eficiência oncológica. OBJETIVO: Demonstrar que os esvaziamentos radicais modificados apresentam um resultado oncológico comparável ao esvaziamento radical clássico. MATERIAL E MÉTODOS: Foram incluídos os pacientes com carcinomas epidermoides do andar inferior de boca e orofaringe, submetidos a esvaziamento cervical radical clássico ou modificado. Foram excluídos desta análise pacientes submetidos a tratamento prévio ou a esvaziamentos cervicais ampliados. DESENHO DO ESTUDO: Retrospectivo, envolvendo uma instituição. RESULTADOS: Identificou-se 481 pacientes que preenchiam os critérios de inclusão, correspondendo a 521 esvaziamentos. O número médio de linfonodos dissecados foi de 44,92 (SD 16.45 linfonodos para o ECR; 44,16 (SD 15,76 para o ECRM +XI e 56,02 (SD 22,91 para o ECRM VJI+XI. A ANOVA indicou uma diferença estatisticamente significativa entre os grupos (pNeck dissection is an integral part of head and neck tumors. Throughout its history, it has undergone changes looking for an improvement in functional outcome without loss of oncologic efficiency. AIM: Demonstrate that the modified radical neck dissection have an oncologic results comparable to classical radical dissection. MATERIALS AND METHODS: We included patients with squamous cell carcinoma of the lower floor of the mouth and oropharynx, who underwent radical classic or modified neck dissection. We excluded from this analysis those patients who had undergone previous treatment or extended neck dissection. STUDY DESIGN: Retrospective study, involving an institution. RESULTS: We identified 481 patients who met the inclusion criteria, corresponding to 521 dissections. The average number of lymph nodes dissected was 44.92 (SD 16:45 lymph nodes to the RCT, 44.16 (SD 15.76 for the MRND + XI

  12. Efeito de EPDM modificado nas propriedades mecânicas e no processamento de composições de EPDM contendo resíduo de EPDMR Effect of modified EPDM on the mechanical properties and processing of EPDM containing EPDM waste

    Directory of Open Access Journals (Sweden)

    Danielli M. Carmo

    2012-01-01

    Full Text Available Neste trabalho foi estudado o efeito de três agentes na recuperação de resíduos de borracha, copolímero de etileno-propylene-dieno (EPDMR contendo negro de fumo. Os três agentes preparados foram: EPDM modificado com 1-dodecanotiol (EPDMSDD, EPDM modificado com ácido esteárico (EPDMAE e EPDM modificado com ácido graxo linolênico (EPDMAG. Os resultados de propriedades mecânicas mostraram que o EPDMSDD fornece a melhor combinação de resistência à tração e rasgamento associado à processabilidade. A adição dos agentes EPDMAE e EPDMAG melhorou a processabilidade da mistura por extrusão, mantendo suas propriedades mecânicas. Este resultado indica que o EPDMAE e o EPDMAG atuam como auxiliares de processamento.This work was aimed at examining the effect from three agents in the recycling of waste rubber, namely copolymer ethylene-propylene-diene (EPDMR containing carbon black. Three agents were prepared: EPDM modified by 1-dodecanotiol (EPDMSDD, EPDM modified by stearic acid (EPDMAE and EPDM modified by fatty linolenic acid (EPDMAG. For the mechanical properties, the better tensile and tear strength associated with manufacturing extrusion were obtained using EPDMSDD. EPDMAE and EPDMAG improved the processing by extrusion, while keeping the mechanical properties. These results indicate that EPDMAE and EPDMAG act as auxiliary agents for processability.

  13. EXPLORACIÓN DE DIFERENCIAS NORMATIVAS EN EL SISTEMA DE CALIFICACIÓN CUALITATIVA PARA EL TEST GESTÁLTICO DE BENDER MODIFICADO/ EXPLORING NORMATIVE DIFFERENCES IN QUALITATIVE SCORING SYSTEM FOR MODIFIED THE BENDER GESTALT TEST

    Directory of Open Access Journals (Sweden)

    César Merino Soto*

    2011-09-01

    Full Text Available RESUMENEl presente estudio explora la magnitud de las diferencias en los puntajes del Sistema de Calificación Cualitativa parael Test Gestáltico de Bender Modificado, usando diferente información normativa proveniente de Perú, Estados Unidos yChina. En una muestra de 324 niños(as peruanos entre 5 y 6 años de edad, se analizaron las potenciales diferencias en ladensidad, tendencia central, dispersión y clasificaciones de rendimiento visomotor. Se hallaron grandes diferenciasnormativas, y por lo tanto, el desempeño en los participantes se vio altamente sobreestimado o subestimado dependiendode la norma usada. Se discute el impacto de estos resultados en la apropiada práctica evaluativa en niños.ABSTRACTThis study explores the magnitude of difference in scores from Qualitative Scoring System to Bender Gestalt TestModified using different normative data from Peru, USA and China. In a sample of 324 children (boys and girls between5 and 6 ages, we analyzed the potential differences in density, central tendency, dispersion and visual motor performanceclassifications. It was found large normative differences, and therefore, performance in participants was highlyoverestimated or underestimated depending on the standard used. It discusses the impact of these results in the appropriateassessment practice in children.

  14. Detecção e quantificação de organismos geneticamente modificados em alimentos e ingredientes alimentares Detection and quantification of genetically modified organisms in food and food ingredients

    Directory of Open Access Journals (Sweden)

    Fabricio Rochedo Conceição

    2006-02-01

    Full Text Available O cumprimento da legislação que regulamenta a comercialização de alimentos e ingredientes contendo Organismos Geneticamente Modificados (OGMs é totalmente dependente da sensibilidade e confiabilidade dos métodos de detecção e quantificação de OGMs. Na presente revisão, foram discutidos os métodos mais relevantes para tais fins, especialmente aqueles que se baseiam na detecção da proteína ou do DNA recombinante, destacando as suas principais propriedades, limitações e vantagens. A regulamentação e algumas sugestões de métodos alternativos para a detecção de OGMs também são abordadas.The enforcement of legislation that regulates the presence of genetically modified organisms (GMOs in food and food ingredients is totally dependent on the sensitivity and reliability of the GMO testing methods. In this review, the most relevant methods such as recombinant proteins or DNA-based methods were discussed, emphasizing their main properties, limitations and advantages. The regulamentation and some suggestions of alternative methods for the detection of GMOs were also discussed.

  15. A problemática dos organismos geneticamente modificados e a formação científica do cidadão comum: um estudo com alunos de escolas portuguesas

    Directory of Open Access Journals (Sweden)

    Luís Dourado

    2014-04-01

    Full Text Available A sociedade atual depara-se com assuntos mediáticos e controversos, de raiz científica, de que é exemplo a problemática sobre os organismos geneticamente modificados (OGM. Estes organismos são, para uns, uma mais-valia e, para outros, algo perigoso, o que tem motivado um debate a nível científico, político e económico, do qual os cidadãos não se devem abster. Para tal, estes indivíduos devem ser pessoas informadas, responsáveis e ativas, aspetos para os quais contribui a formação em ciências. A investigação realizada centrou-se na problemática dos OGMs e envolveu a aplicação de um questionário a 175 alunos de escolas portuguesas sobre as suas concepções e opiniões em relação aos OGM. Este estudo demonstrou que a maioria dos alunos tem ideias vagas, nem sempre cientificamente corretas, sobre o tema, e que não rejeita os OGMs, apesar de não ter muitas certezas em relação à utilização dos mesmos.

  16. Ankara'da çeşitli kaynaklardan satın alınan beyaz peynirlerin mikrobiyolojik kalite kontrolü üzerinde araştırmalar

    OpenAIRE

    URHAN, Gülay

    2012-01-01

    Bu çalışmada, 2011 yılı Temmuz-Eylül ayları arasında Ankara ilinin çeşitli semtlerindeki marketlerde ve halk pazarlarında satışa sunulan 50 adet beyaz peynir örneği mikrobiyolojik özellikleri bakımından incelenmiştir.Mikrobiyolojik analizler sonucunda örneklerde ortalama toplam mezofil aerobik bakteri sayısı 5,8 x 106 kob/g, maya ve küf sayısı 1,2 x 106 kob/g ve koliform bakteri sayısı ise 5,4 x 102 EMS/g olarak tespit edilmiştir. Ayrıca her bir örnekte E. coli, S. aureus...

  17. Ankara Ekolojik Şartlarında Farklı Ekim Zamanlarının Ketencik (Camelina sativa (L.) Crantz) Bitkisinin Verim Ve Verim Unsurları Üzerine Etkisi

    OpenAIRE

    Katar, Duran; Arslan, Yusuf; Subaşı, İlhan

    2013-01-01

    ÖZET : Bu çalışma, Ankara ekolojik koşullarında 2010 ve 2011 yıllarında farklı ekim zamanın ketencik (Camelina sativa (L.) Crantz) bitkisinin bitki boyu (cm), bin tohum ağırlığı (g), dekara tohum verimi (kg/da) ve yağ oranı (%) üzerine etkisini belirlemek amacıyla yapılmıştır. Deneme Tesadüf Blokları deneme desenine göre üç tekerrürlü olarak kurulmuştur. Bitki boyunda (cm), bin tohum ağırlığında (g), dekara tohum veriminde (kg/da) ve yağ oranı ...

  18. PRODUCT IRON-BEARING CONTROL TECHNOLOGY AND MEASURES FOR 25.5 MVA INDUSTRIAL SILICON FURNACE%25.5 MVA工业硅炉产品含铁量控制工艺及措施

    Institute of Scientific and Technical Information of China (English)

    赵兴凡; 周杰; 王子龙; 卢国洪

    2015-01-01

    In order to effectively control industrial silicon product iron-bearing, it analyzes and elaborates iron-bearing resource for 25.5 MVA industrial silicon furnace. It makes process treatment from raw materials, operation and products, obtaining the key factors affected the products’ quality. At the same time, it puts forward research area and prospect for future improvement on industrial silicon products.%为了有效控制工业硅产品的铁含量,分析和阐述了25.5 MVA工业硅炉产品中铁元素的来源,对其主要生产原料、生产操作工艺及产品进行了技术工艺处理,找出了影响产品质量的关键因素,同时也对未来工业硅产品质量技术的提升提出了一些研究方向和展望。

  19. Seasonal variation of surface temperature based on land cover in AnkaraAnkara şehrinde yüzey sıcaklıklarının arazi örtüsüne göre mevsimsel değişimi

    Directory of Open Access Journals (Sweden)

    İhsan Çiçek

    2013-03-01

    Full Text Available In this study, the seasonal variation of the surface temperature of Ankara urban area and its enviroment have been analyzed by using Landsat 7 image. The Landsat 7 images of each month from 2007 to 2011 have been used to analyze the annually changes of the surface temperature. The land cover of the research area was defined with supervised classification method on the basis of the satellite image belonging to 2008 July. After determining the surface temperatures from 6-1 bands of satellite images, the monthly mean surface temperatures were calculated for land cover classification for the period between 2007 and 2011. Accordşng to the results obtained, the surface temperatures are high in summer and low in winter from the air temperatures. all satellite images were taken at 10:00 am, it is found that urban areas are cooler than rural areas at 10:00 am. Regarding the land cover classification, the water surfaces are the coolest surfaces during the whole year. The warmest areas are the grasslands and dry farming areas. While the parks are warmer than the urban areas during the winter, during the summer they are cooler than artificial land covers. The urban areas with higher building density are the cooler surfaces after water bodies. ÖzetBu çalışmada, Ankara şehri ve çevresindeki yüzey sıcaklıklarının yıl içerisindeki değişimi incelenmiştir. 2007-2011 yılları arasında yılın tüm aylarına bir görüntü düşecek şekilde, Landsat 7 uydusu ile alınan görüntülerin kullanıldığı çalışmada, 2008 yılı temmuz ayı görüntüsünden kontrollü sınıflandırma yöntemiyle şehir ve çevresine ait arazi örtüsü oluşturulmuştur. Uydu görüntülerinin 6-1 termal bantından  yüzey sıcaklıkları hesap edildikten sonra, arazi örtülerinin her aya ait ortalama yüzey sıcaklıkları belirlenmiştir. Elde edilen değerlere göre yüzey sıcaklıkları, hava sıcaklığına paralel olarak, yaz aylarında yüksek, k

  20. Comunidade bacteriana como indicadora do efeito de feijoeiro geneticamente modificado sobre organismos não alvo Bacterial community as an indicator of genetically modified common bean effect on nontarget organisms

    Directory of Open Access Journals (Sweden)

    Adriano Moreira Knupp

    2009-12-01

    Full Text Available O objetivo deste trabalho foi avaliar o efeito do feijoeiro geneticamente modificado quanto à resistência ao Bean Golden Mosaic Vírus, BGMV (Olathe M1-4, sobre organismos não alvo. De um experimento implantado no campo, em delineamento inteiramente casualizado, com dois tratamentos (Olathe Pinto e evento elite Olathe M1-4, dois períodos amostrais (estádio V4 e R6 e dez repetições, obtiveram-se células bacterianas cultivadas e não cultivadas da rizosfera e do solo não rizosférico, para as quais se procedeu à extração de DNA total. A região V6-V8 do 16S rDNA foi amplificada para a comunidade bacteriana total, e também realizou-se amplificação com iniciadores específicos para o subgrupo alfa (α do filo Proteobacteria a partir de células não cultivadas. Foram obtidos dendrogramas comparativos entre a variedade Olathe Pinto (convencional e o evento elite Olathe M1-4 (geneticamente modificado utilizando-se o coeficiente de Jaccard e o método UPGMA (Unweighted pair-group method with arithmetic mean. Os agrupamentos obtidos dos perfis de 16S rDNA PCR-DGGE indicam alterações na comunidade bacteriana da rizosfera em função da transformação das plantas são mais notáveis nos perfis obtidos para alfa-proteobacteria. A origem das amostras e o estágio de desenvolvimento das plantas afetam a comunidade bacteriana.The objective of this work was to evaluate the effect of genetically modified common bean for Bean Golden Mosaic Virus, BGMV, resistance (Olathe M1-4 on nontarget organisms. In a field experiment established in a completely randomized design with two treatments (Olathe Pinto cultivar and M1-4 Olathe elite event, two sampling periods (V4 and R6 stages and ten replicates, cultivated and non-cultivated bacterial cells from rhizosphere soil and bulk soil were obtained, and their total DNA was extracted. The V6-V8 region of 16S rDNA was amplified for the whole bacterial community, and primers specific for the alpha (

  1. Consumer preferences of genetically modified foods of vegetal and animal origin in Chile Preferências dos consumidores aos alimentos geneticamente modificados de origem animal e vegetal no Chile

    Directory of Open Access Journals (Sweden)

    Berta Schnettler

    2012-03-01

    Full Text Available Given the debate generated by Genetically Modified (GM foods in developed and developing countries, the aim was to evaluate the importance of determining factors in the preference of consumers in Temuco and Talca in central-southern Chile for GM foods using conjoint analysis and to determine the existence of different market segments using a survey of 800 people. Using conjoint analysis, it was established that, in general, genetic modification was a more important factor than either brand or price in the consumer's decision to purchase either food. Cluster analysis identified three segments: the largest (51.4% assigned greatest importance to brand and preferred genetically modified milk and tomato sauce; the second group (41.0% gave greatest importance to the existence of genetic manipulation and preferred non-genetically modified foods; the smallest segment (7.6% mainly valued price and preferred milk and tomato sauce with no genetic manipulation. The three segments rejected the store brand and preferred to pay less for both foods. The results are discussed based on studies conducted in developed and developing countries.Com base no debate gerado pelos alimentos geneticamente modificados (GM, tanto em países desenvolvidos como em países em desenvolvimento, a partir do uso da conjoint analysis, o objetivo foi avaliar a importância dos fatores determinantes na preferência de alimentos GM pelos consumidores das cidades de Temuco e Talca, zona Centro-Sul do Chile, e a existência de diferentes segmentos de mercado, mediante uma enquete a 800 pessoas. Utilizando conjoint analysis, se determinou, em geral, que a existência de modificação genética foi mais importante que a marca e o preço na decisão de compra de ambos os alimentos. Mediante análise cluster, se distinguiram três segmentos, o mais numeroso (51,4% deu leve maior importância à marca e preferiu leite e molho de tomate geneticamente modificado. O segundo grupo (41,0% deu

  2. ELEMENTOS PARA EL DISEÑO DE UN SISTEMA DE INFORMACIÓN PARA LA TOMA DE DECISIONES EN ACTIVIDADES RELACIONADAS CON ORGANISMOS GENÉTICAMENTE MODIFICADOS: APORTES A PARTIR DE UN CASO DE ESTUDIO

    Directory of Open Access Journals (Sweden)

    BENAVIDES-MOLINEROS JULIA

    2012-07-01

    Full Text Available En Colombia, las actividades relacionadas con organismos genéticamente modificados (OGM deben estar respaldadas por evaluaciones de los riesgos que ellas puedan representar para la diversidad biológica, la salud humana y la producción agropecuaria. Con base en estas evaluaciones, las autoridades competentes toman decisiones consistentes principalmente en autorizar o negar las actividades solicitadas.En estas decisiones, la racionalidad del proceso está bastante bien determinada en lo que respecta a los efectos sobre la salud humana, en particular toxicidad y alergenicidad, pero no ocurre lo mismo con los efectos sobre la biodiversidad. Uno de los mayores problemas en este campo es la falta de definición de una metodología específica para la toma de decisiones, lo cual da como resultado que las decisiones sean tomadas de manera intuitiva y poco sistemática. Las autoridades competentes han reconocido la necesidad de contar con un sistema de información que contribuya a resolver esta situación.Este trabajo presenta una propuesta de la estructura básica de un sistema para la toma de decisiones orientado a las autoridades involucradas en el proceso. La propuesta se definió con base en una revisión de las principales metodologías para la evaluación de riesgos en materia de OGM y tomando como caso de estudio el flujo de genes desde OGM hacia parientes silvestres. La estructura está planteada como un modelo entidad-relación de carácter general a partir del cual puede desarrollarse el diseño detallado del sistema. La propuesta hace énfasis en la documentación de los protocolos de decisión y de la racionalidad de uso de los insumos de información.

  3. Uso do índice de Kotelchuck modificado na avaliação da assistência pré-natal e sua relação com as características maternas e o peso do recém-nascido no Município do Rio de Janeiro

    Directory of Open Access Journals (Sweden)

    Maria do Carmo Leal

    2004-01-01

    Full Text Available O índice de Kotelchuck (IK foi modificado e utilizado para avaliar a assistência pré-natal prestada no Município do Rio de Janeiro - Brasil, em uma amostra de 9920 puérperas de parto único. Regressões multivariadas logística ordinal (RMLO e linear (RML foram utilizadas para estimar a importância de fatores demográficos, psicossociais e obstétricos no IK modificado e seus efeitos no peso ao nascer (PN, respectivamente. Apenas 38,5% das parturientes do Município do Rio de Janeiro foram classificadas como de cuidado pré-natal adequado ou intensivo. Após ajustamento por outros preditores, mantiveram-se como variáveis explicativas do IK: o nível de instrução, viver com o pai do RN, tentar abortar, diabetes, satisfação com a gravidez, cor da pele, paridade, idade e local de residência. O PN associou-se com o IK modificado, mesmo após o controle de variáveis sociodemográficas, comportamentais e biológicas. A utilização adequada da assistência pré-natal no Município do Rio de Janeiro contribuiu na prevenção do PN e as mães que menos utilizaram os serviços pré-natais têm piores condições socioeducacionais, de apoio familiar e de risco obstétrico.

  4. Applications of high-Tc-superconductors to power engineering. Manufacture of YBCO plate-type conductors and construction of a HTS current limiter model up to 1 MVA nominal power. Final report

    International Nuclear Information System (INIS)

    In terms of materials, the main focus of the work was on the manufacture of large-area YBCO plate-type conductors with homogeneous properties and maximum current densities of jc>1 MA/cm2. jc values of better than 3 MA/cm2 were achieved reproducibly on sapphire substrates of 100 mm diameter and 10 x 20 cm2 in size with a homogeneity of 10%; on polycrystalline substrates of 10 x 20 cm2 in size, homogeneous jc values of up to 2 MA/cm2 were also successfully demonstrated. Of the total of four methods of coating available at the start of the project, thermal co-evaporation (TCE) proved best for YBCO thin films and the IBAD method best for quasi single-crystal buffer films. The latter are necessary to achieve high jc on polycrystalline substrates such as ZrO2(Y), glass and Al2O3. Polycrystalline substrates are essential in order to make the HTS current limiter as a future product commercially feasible. The favoured solutions ZrO2(Y) and glass have not come up to expectations, because present investigations into quench propagation are showing that, with this approach, the high values of power density required for the switching process (1600 VA/cm2) cannot be achieved. Towards the end of the project, polycrystalline Al2O3 began to be seen as a successful alternative; the work is being pursued further within the context of a follow-on project. The coating processes were stabilized successfully and, when combined with strict quality control, allowed the yield of tested, ready-to-use plate-type conductors to be improved to 85%. This success was an essential prerequisite for the building of a 3-phase, 1.2 MVA model (7.2 kV) comprising a total of sixty-three 100 mm plate-type conductors. At the Berlin factory the model has been successfully tested up to a prospective short-circuit current of 5 kV. This has demonstrated the basic suitability of HTS thin-film technology for use in current limiters. So far the model has been switched a total of 43 times without any problems at all

  5. Caracterización físico-mecánica, térmica y morfológica de polímeros de redes interpenetradas con base en poliuretano obtenido a partir de aceite de ricino y almidón modificados/polimetilmetacrilato (PMMA)

    OpenAIRE

    Manuel F. Valero; Jorge E. Pulido; Álvaro Ramírez; Diana C. Camargo; Daniel Navas

    2011-01-01

    El aceite de ricino se modificó por transesterificación con pentaeritritol. Paralelamente, se utilizó la reacción de glucosilación con glicerol para dividir el almidón en unidades monosacáridos. El glucósido obtenido reaccionó por transesterificación con el aceite modificado por transesterificación con pentaeritritol. Se determinó el índice de hidroxilo de los poliol-glucósidos en función del contenido de pentaeritritol y almidón utilizados en la síntesis. Se sintetizaron redes interpenetrada...

  6. 100 years of thermal waste treatment - off-gas purification technology then and now. Performance results of the Stellinger Moor waste incineration plant at Hamburg; 100 Jahre thermische Abfallbehandlung - Abgasreinigungstechnik damals und heute, Betriebserfahrungen der MVA Stellinger Moor, Hamburg

    Energy Technology Data Exchange (ETDEWEB)

    Franck, J. [Stadtreinigung Hamburg, MVA Stellinger Moor, Hamburg (Germany); Schellenberger, I. [Goepfert, Reimer und Partner, Hamburg (Germany); Karpinski, A. [Lentjes Energietechnik GmbH, Essen (Germany)

    1997-06-01

    The contribution outlines the history of thermal waste treatment, starting from the first such plant constructed at Hamburg-Bullerdeich. It also goes into the social context in which the decision to construct such a plant was made. As an example of a modern, up-to-date system, the Stellinger Moor plant at Hamburg is described for comparison. The flue gas purification process employed here reflects the most recent state of the art. If the technical and social boundary conditions of 100 years ago are compared with those of today, one sees how far plant technology has advanced since the first years, especially in the field of emission reduction. On the other hand, the acceptance problems facing operators of thermal waste incineration systems are still the same as 100 years ago. [Deutsch] Dieser Beitrag vermittelt einen Eindruck ueber die Anfaenge der thermischen Abfallbehandlung. Es wird gezeigt, mit welchen technischen Raffinessen die erste Anlage am Bullerdeich in Hamburg errichtet und betrieben wurde. Daneben wird das gesellschaftliche Umfeld angerissen, in dem die Entscheidung fuer eine derartige Behandlungsanlage reifte. Um einen Vergleich mit der heutigen, modernen Anlagentechnik zu ermoeglichen, wird die MVA Stellinger Moor in Hamburg technisch und betrieblich vorgestellt. Das angewandte technische Verfahren der Abgasreinigung entspricht voll und ganz den heutigen hohen Erwartungen der Gesetzgebung. Vergleicht man die Randbedingungen von vor 100 Jahren mit den heutigen sowohl in technischer als auch in gesellschaftlicher Sicht, so ist festzustellen, dass es eine gewaltige Entwicklung bei der Anlagentechnik, besonders vor dem Hintergrund der Reduzierung von Schadgasemissionen, gegeben hat. Die Akzeptanzprobleme fuer thermische Behandlungsanlagen sind aber heute noch genauso vorhanden wie vor 100 Jahren. (orig.)

  7. Fundamental studies on storage in saline caverns of thickened chemical and toxic waste, especially incinerator ash. Final report and summary; Grundlagenuntersuchungen zum Dickstoffverfahren mit chem./tox. Abfaellen, insbesondere MVA-Filteraschen, im Salinar. Zusammenfassender Schlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Kecke, H.J. (ed.)

    2001-07-01

    mit chem./tox. Abfaellen, insbesondere MVA-Filteraschen, im Salinar' (1997-2000). (orig.)

  8. Ankara İli Merkez İlçede Bireylerin Gazlı İçecek Tüketimine İlişkin Bir Değerlendirme

    OpenAIRE

    Bilge Gözener; Murat Sayılı

    2015-01-01

    Bu araştırmada, Ankara ili Merkez ilçede (Çankaya, Etimesgut, Gölbaşı, Keçiören, Mamak, Polatlı, Sincan ve Yenimahalle ilçeleri) tüketicilerin gazlı içecek tüketim durumları incelenmiştir. Araştırmada 272 aileden anket yolu ile elde edilen veriler kullanılmıştır. Veriler Nisan-Mayıs-2012 yılına aittir. Tüketicilerin bazı demografik özellikleri ile gazlı içecek tüketimleri arasında istatistiksel bir ilişki olup olmadığı ki-kare analizi ile test edilmiştir. Araştırma sonuçlarına göre; ailelerin...

  9. Design and simulation of 3½-cell superconducting gun cavity and beam dynamics studies of the SASE-FEL System at the Institute of Accelerator Technologies at Ankara University

    International Nuclear Information System (INIS)

    Design and simulation of a superconducting gun cavity with 3½ cells have been studied in order to give the first push to the electron beam for the linear accelerating system at The Institute of Accelerator Technologies at Ankara University. Electrons are accelerated through the gun cavity with the help of the Radiofrequency power suppliers from cryogenic systems. Accelerating gradient should be as high as possible to accelerate electron beam inside the cavity. In this study, electron beam reaches to 9.17 MeV energy at the end of the gun cavity with the accelerating gradient; Ec=19.21 MV/m. 1.3 GHz gun cavity consists of three TESLA-like shaped cells while the special designed gun-cell includes a cathode plug. Optimized important beam parameters inside the gun cavity, average beam current 3 mA, transverse emittance 2.5 mm mrad, repetition rate 30 MHz and other parameters are obtained for the SASE-FEL System. The Superfish/Poisson program is used to design each cell of the superconducting cavity. Superconducting gun cavity and Radiofrequency properties are studied by utilizing 2D Superfish/Poisson, 3D Computer Simulation Technology Microwave Studio, and 3D Computer Simulation Technology Particle Studio. Superfish/Poisson is also used to optimize the geometry of the cavity cells to get the highest accelerating gradient. The behavior of the particles along the beamline is included in this study. ASTRA Code is used to track the particles

  10. Late Jurassic-Early Cretaceous radiolarian age constraints from the sedimentary cover of the Amasia ophiolite (NW Armenia), at the junction between the Izmir-Ankara-Erzinçan and Sevan-Hakari suture zones

    Science.gov (United States)

    Danelian, T.; Asatryan, G.; Galoyan, Gh.; Sahakyan, L.; Stepanyan, J.

    2016-01-01

    The Amasia ophiolite, situated at the northernmost corner of Armenia, is part of the Sevan-Hakari suture zone which links with the Izmir-Ankara-Erzinçan suture zone in northern Turkey. Three new radiolarian assemblages have been extracted from siliceous sedimentary rocks that accumulated on the Amasia ophiolite in an oceanic setting. Two of these assemblages were extracted from red-brownish bedded cherts overlying basaltic lavas; one of these is likely to be middle Oxfordian to early Kimmeridgian in age, while the second correlates with the Berriasian. Similar time-equivalent lava-chert sequences have been dated recently using radiolarians from the Stepanavan, Vedi and Sevan ophiolite units, where they are considered to relate to submarine volcanic activity in the back-arc marginal basin in which the Armenian ophiolites were formed. The third radiolarian assemblage, of late Barremian age, was extracted from a more than 15-m-thick volcaniclastic-chert sequence. The related volcanic activity is likely to have been subaerial and probably relates to the formation of an oceanic volcanic plateau; no Cretaceous subaerial volcanism has been previously recorded in the Lesser Caucasus area.

  11. Design and simulation of 3½-cell superconducting gun cavity and beam dynamics studies of the SASE-FEL System at the Institute of Accelerator Technologies at Ankara University

    Science.gov (United States)

    Yildiz, H. Duran; Cakir, R.; Porsuk, D.

    2015-06-01

    Design and simulation of a superconducting gun cavity with 3½ cells have been studied in order to give the first push to the electron beam for the linear accelerating system at The Institute of Accelerator Technologies at Ankara University. Electrons are accelerated through the gun cavity with the help of the Radiofrequency power suppliers from cryogenic systems. Accelerating gradient should be as high as possible to accelerate electron beam inside the cavity. In this study, electron beam reaches to 9.17 MeV energy at the end of the gun cavity with the accelerating gradient; Ec=19.21 MV/m. 1.3 GHz gun cavity consists of three TESLA-like shaped cells while the special designed gun-cell includes a cathode plug. Optimized important beam parameters inside the gun cavity, average beam current 3 mA, transverse emittance 2.5 mm mrad, repetition rate 30 MHz and other parameters are obtained for the SASE-FEL System. The Superfish/Poisson program is used to design each cell of the superconducting cavity. Superconducting gun cavity and Radiofrequency properties are studied by utilizing 2D Superfish/Poisson, 3D Computer Simulation Technology Microwave Studio, and 3D Computer Simulation Technology Particle Studio. Superfish/Poisson is also used to optimize the geometry of the cavity cells to get the highest accelerating gradient. The behavior of the particles along the beamline is included in this study. ASTRA Code is used to track the particles.

  12. Design and simulation of 3½-cell superconducting gun cavity and beam dynamics studies of the SASE-FEL System at the Institute of Accelerator Technologies at Ankara University

    Energy Technology Data Exchange (ETDEWEB)

    Yildiz, H. Duran, E-mail: hdyildiz@ankara.edu.tr [Institute of Accelerator Technologies, Ankara University, Ankara (Turkey); Cakir, R. [Nanotechnology Engineering Department, Recep Tayyip Erdogan University, Rize (Turkey); Porsuk, D. [Physics Department, Dumlupinar University, Kutahya (Turkey)

    2015-06-11

    Design and simulation of a superconducting gun cavity with 3½ cells have been studied in order to give the first push to the electron beam for the linear accelerating system at The Institute of Accelerator Technologies at Ankara University. Electrons are accelerated through the gun cavity with the help of the Radiofrequency power suppliers from cryogenic systems. Accelerating gradient should be as high as possible to accelerate electron beam inside the cavity. In this study, electron beam reaches to 9.17 MeV energy at the end of the gun cavity with the accelerating gradient; E{sub c}=19.21 MV/m. 1.3 GHz gun cavity consists of three TESLA-like shaped cells while the special designed gun-cell includes a cathode plug. Optimized important beam parameters inside the gun cavity, average beam current 3 mA, transverse emittance 2.5 mm mrad, repetition rate 30 MHz and other parameters are obtained for the SASE-FEL System. The Superfish/Poisson program is used to design each cell of the superconducting cavity. Superconducting gun cavity and Radiofrequency properties are studied by utilizing 2D Superfish/Poisson, 3D Computer Simulation Technology Microwave Studio, and 3D Computer Simulation Technology Particle Studio. Superfish/Poisson is also used to optimize the geometry of the cavity cells to get the highest accelerating gradient. The behavior of the particles along the beamline is included in this study. ASTRA Code is used to track the particles.

  13. İş tatmini ile stres düzeyleri ilişkisi: Ankara Gölbaşı Hasvak Devlet Hastanesi ve Kalecik Devlet Hastaneleri örneği

    OpenAIRE

    ARIZ, Tuğba

    2010-01-01

    Bu araştırma Ankara Gölbaşı Hasvak ile Kalecik Devlet Hastanelerinde görev yapan hastane personelinin iş tatmini ve stres düzeyleri arasındaki ilişkinin belirlenmesi amacıyla yapılmıştır. Araştırma kapsamına hastanede çalışan tüm personel dahil edilmiştir. Toplamda 276 personele anket uygulanmıştır. Evrenin % 86'sına ulaşılmıştır. Veriler anket yöntemiyle yüz yüze görüşme tekniğiyle elde edilmiştir. Uygulama Eylül 2009 tarihlerinde yapılmıştır. İş tatminini belirleyebilme...

  14. Ankara Piyasasında Satılan Ayran ve Yoğurt Örneklerinin Hijyenik Kaliteleri Üzerinde Araştırmalar

    OpenAIRE

    Duru, Suna; Özgüneş, Hilal

    1981-01-01

    Birçok ülkede süt ve süt ürünleri kişinin diyetinin önemli bir bölümünü oluşturmaktadır. Bu gıda maddelerinin hastalık taşıma potansiyeli yüksek olduğundan, hijyenik kalitelerine özen gösterilmesi gerekir. Bu çalışmada, yurdumuzda büyük ölçüde tüketilen ürünlerden ayran ve yoğurt, hijyenik kaliteleri yönünden incelendi. 9 Haziran-14 Ağustos 1979 tarihleri arasında çeşitli zamanlarda Ankara piyasasından toplanan 50 ayran ve 20 yoğurt örneğinde koliform bakteri sayımı, 40 ayran ve 20 yoğurt ö...

  15. T. C. Kültür ve Turizm Bakanlığı Ankara Devlet Klâsik Türk Müziği Korosunun Repertuarının İncelenmesi

    OpenAIRE

    Kaynarca, Burak

    2005-01-01

    Bu çalışmada, ülkemizin kültür ve sanat hayatının en yetkin kurumlarından olan Kültür ve Turizm Bakanlığı Ankara Devlet Klâsik Türk Müziği Korosunun kuruluşundan bu yana gerçekleştirilmiş tüm konser repertuarları incelenmiş, seçilen repertuarlarda hangi dönem Türk Mûsikîsi eserlerine ağırlık verildiği, en çok hangi formdan eserlerin tercih edildiği saptanmıştır. Ayrıca Kültür ve Turizm Bakanlığına bağlı Güzel Sanatlar Müdürlüğünün ve ona bağlı Türk Müziği Korolarının kuruluş amaçları ve günüm...

  16. ANKARA EKOLOJİK KOŞULLARINDA FARKLI AYÇİÇEĞİ (Helianthus annuus L.) ÇEŞİTLERİNİN VERİM PERFORMASLARININ BELİRLENMESİ

    OpenAIRE

    BAYRAMİN, Suay; KAYAÇETİN, Fatma; Arslan, Yusuf; KATAR, Duran

    2012-01-01

    Bu çalışma, 2009 yılında Ankara/Haymana ekolojik koşullarında yürütülmüştür. Çalışmada, 7 farklı hibrit ayçiçeği çeşidi (A71, M69, Califa, Oleko, Oliva, Sanay ve Sanbro) tohumu materyal olarak kullanılmıştır. Denemede; bitki boyu (cm), tabla çapı (cm), tohum verimi (kg/da), yağ oranı (%) ve yağ verimi (kg/da) belirlenmiştir. Araştırmada, çeşitlere bağlı olarak bitki boylarının 101,77 – 127,53 cm, tabla çaplarının 12,67 -14,57 cm, tohum veriminin 135,5 – 240,6 kg/da, yağ oranını % 36,83 – 46,...

  17. Método sol-gel modificado para obtenção de alumina nanoencapsulada com terras raras Sol-gel modified method for obtaining alpha-alumina nanocoated with rare earth

    Directory of Open Access Journals (Sweden)

    A. P. Maciel

    2005-03-01

    Full Text Available Neste trabalho, alfa-alumina foi nanoencapsulada com óxido de lantânio usando um método sol-gel modificado. Um precursor polímero de lantânio foi usado para encapsular a alfa-Al2O3, dispersa em água. A influência da nanocápsula de óxido de lantânio na sinterização e microestrutura da alfa-alumina foi investigada usando-se técnicas de microscopia eletrônica. A microscopia eletrônica de varredura dos grãos nanoencapsulados revelou que compostos de lantânio precipitam no contorno de grão da alfa-Al2O3. A microscopia eletrônica de alta resolução mostrou claramente a formação de uma camada de La2O3 na superfície da alumina. A camada de La2O3 modifica o processo de sinterização da alumina pura. Em temperaturas elevadas ocorrem mudanças significativas na morfologia e microestrutura da alfa-alumina. Verificou-se que a camada amorfa de La2O3 reage com a alumina, resultando em LaAlO3, aumentando a temperatura de sinterização. A fase La2Al24,4O39,6 foi observada no material sinterizado.alpha-alumina powder was nanocoated with lanthanum oxide by sol-gel like method. A lanthanum polymeric precursor was used to coat the alpha-Al2O3 dispersed in water. The influence of the lanthanum oxide nanocoating on the sintering and microstructure of alpha-alumina was investigated using electron microscopy techniques. Scanning electron microscopy (SEM of these nanocoated grains revealed that the lanthanum oxide precipitates on the grain boundary of the alpha-alumina, at high temperature. High-resolution electron microscopy (HREM observations clearly showed the formation of a La2O3 layer on the surface of the alpha-alumina. The La2O3 layer modified the sintering process of the pure alumina. At high temperatures significant modifications on the grain morphology and microstructure was observed. It was verified that an amorphous La2O3 layer reacts with the alpha-alumina grain, resulting in LaAlO3 and increasing the sintering temperature. The La2Al

  18. Uso de fluorescência em um método de dissector modificado para estimar o número de miócitos no tecido cardíaco

    Directory of Open Access Journals (Sweden)

    Rômulo Dias Novaes

    2012-03-01

    Full Text Available FUNDAMENTO: Métodos convencionais de dissector atualmente requerem consideráveis custos financeiros, técnicos e operacionais para estimar o número de células, incluindo cardiomiócitos, em uma área de 3D. OBJETIVO: Usar a microscopia de fluorescência em um método de dissector modificado para determinar o número de miócitos no tecido cardíaco em condições normais e patológicas. MÉTODOS: O estudo empregou camundongos Wistar machos com quatro meses de idade e peso de 366,25 ± 88,21 g randomizados em grupos controles (GC, n = 8 e infectados (GI, n = 8. Os animais do GI foram inoculados com cepa Y de T. cruzi (300.000 tripomastigotas/50 g. Após oito semanas, os animais foram pesados e sacrificados. Os Ventrículos Esquerdos (VE foram removidos para análise estereológica da densidade numérica de cardiomiócitos (Nv [c] e o número total dessas células no VE (N [c]. Esses parâmetros foram estimados usando um dissector fluorescente (DF e comparados com os métodos convencionais de dissector óptico (DO e dissector físico (DFi. RESULTADOS: Em ambos os métodos de dissector, os animais do GI apresentaram queda significativa de Nv[c] e N[c] em comparação com os animais do GC (P > 0,05. Uma correlação forte, igual ou superior a 96%, foi obtida entre DF, DO e DFi. CONCLUSÃO: O método DF parece ser igualmente confiável para determinar Nv[c] e N[c] em condições normais e patológicas, apresentando algumas vantagens em relação aos métodos convencionais de dissector: redução de cortes histológicos e imagens na análise estereológica, redução do tempo de análise das imagens, a construção de DF em microscópios simples, utilizando o modo de epifluorescência, distinção de planos de dissector em ampliações inferiores.

  19. Efecto de los ciclos térmicos de soldadura por arco eléctrico indirecto modificado (AEIM en las propiedades mecánicas de la aleación AA6061-T6

    Directory of Open Access Journals (Sweden)

    Ambriz, Ricardo R.

    2009-02-01

    Full Text Available Results of temperature measurements during welding of 12.7 mm thick AA6061-T6 alloy plates by modified indirect electric arc (MIEA are presented. This study describes the thermal cycles of the heat affected zone (HAZ and also in the fusion zone. Depending upon the position of the transducers, the maximum temperatures measured in the HAZ range from 308 to 693 °C, these measurements were related with the tensile test results, and the failure zone reported previously by the authors [1]. It was observed that, there is a decrease in the mechanical strength of the welded joints, due to the microstructural changes suffered by AA6061-T6 alloy in which formation of the β’ occurs according to the TTT transformation diagram. The inherent cooling conditions of the weld pool observed for the MIEA technique (only one pass of welding, have permitted to establish the characteristics of solidification and microstructure for a specific cooling rate.

    Se presentan los resultados de medición de temperatura durante la soldadura por arco eléctrico indirecto modificado (AEIM de la aleación AA6061-T6. Éstas, describen los ciclos térmicos de la zona afectada por el calor (ZAC y en la zona de fusión. Los resultados muestran que las temperaturas máximas medidas en la ZAC se encuentran en un rango de 308 a 693 °C, dependiendo de la posición de los sensores, estas mediciones fueron correlacionadas con los resultados de resistencia a la tracción y la zona de fallo, reportados previamente por los autores[1]. Se observó que existe una disminución en la resistencia mecánica de las uniones, debido a los cambios microestructurales por la formación de precipitados sobreenvejecidos, β’, de acuerdo con su diagrama de transformación (TTT. Las condiciones de enfriamiento en el baño fundido inherentes a la técnica por AEIM (un solo paso de soldadura, permitieron establecer las características de solidificación y microestructura esperada

  20. Utilização de um contêiner modelo Celle modificado para resfriamento e transporte de sêmen eqüino Use of a Celle modified container for equine semen cooling and transportation

    Directory of Open Access Journals (Sweden)

    G.R. Valle

    1999-10-01

    Full Text Available Desenvolveu-se o contêiner modelo Celle modificado para o resfriamento e transporte de sêmen eqüino. O contêiner possibilitou a inseminação de 100 éguas de diversas categorias reprodutivas, em 148 ciclos estrais. Foram utilizadas doses inseminantes de 400 milhões de espermatozóides móveis em 15ml de sêmen diluído em diluidor de leite em pó desnatado/glicose, transportado por um período médio de 215 minutos e temperatura final de 14ºC. As taxas de concepção ao primeiro ciclo e concepção/ciclo foram de 56 e 52%, respectivamente. O contêiner propiciou, durante seu uso em campo, taxa de resfriamento de -0,03ºC/min entre 19ºC e 9ºC, faixa do choque térmico. No entanto, avaliada em condições de laboratório, a taxa de resfriamento foi mais acelerada. O contêiner utilizado permite o resfriamento simultâneo de oito doses inseminantes individualizadas e com mínima aeração.A modified Celle container was developed for equine semen cooling and transportation. It was possible to inseminate 100 mares with 148 estral cycles. Each inseminating dose contained 400 million motile spermatozoa in 15ml extended semen in dry skim milk-glucose extender, transported for 215 min at 14ºC. The conception rates, at first cycle, and conception/cycle were 56 and 52%, respectively. During a field trial, the container propitiated a cooling rate of -0,03ºC/min between 19ºC and 9ºC, the cold shock zone. However, in a laboratory assessment the cooling rate was more accelerated. The container allowed cooling, simultaneously, of eight individual insemination doses with minimal aeration.

  1. Degradación Heliofotocatalítica de Escherichia coli en Sistemas tipo Desinfección SODIS, con Dióxido de Titanio Modificado Escherichia coli Heliophotocatalytic Degradation in Solar Disinfection Systems SODIS, using Modified Titanium Dioxide

    Directory of Open Access Journals (Sweden)

    Camilo A Castro

    2009-01-01

    Full Text Available Se presenta un estudio sobre la desinfección heliofotocatalítica de agua (DHFCA en sistemas tipo desinfección solar (Solar Disinfection, SODIS. Se usaron fotocatalizadores basados en dióxido de titanio (TiO2 comercial y TiO2 obtenido por el método sol-gel modificados con metales de transición (Ag, Fe, Mo o Pd. Los resultados muestran que la unión de la técnica SODIS con la fotocatálisis permite disminuir el tiempo de exposición a la radiación solar de unidosis de agua infectada, comparado con el necesario para la desinfección total cuando se utiliza el sistema SODIS solo. Además, se encontró que la eficiencia de los fotocatalizadores evaluados en la DHFCA depende del método de preparación y de la naturaleza del metal incorporado. La incorporación de Ag o Pd aumenta la acción bactericida de la DHFCA. La plata presenta una acción adicional a la fotocatalítica debido a sus propiedades bacteriostáticas, mientras que el Pd mejora la fotoactividad gracias a sus propiedades electrónicas.A study concerning the Heliophotocatalytic Disinfection of Water (HPDW, in Solar Disinfection type systems (SODIS is presented. Photocatalysts based on a commercial titanium dioxide (TiO2 and TiO2 obtained by the sol-gel method both modified with transition metals (Ag, Fe, Mo or Pd, were used. Results show that the combination of the SODIS technique with photocatalysis reduces the solar irradiation time exposure of a unidosis of infected water, when compared to the one needed using the SODIS technique alone. In addition, it was observed that both, the synthesis method and the nature of the metal modifying the TiO2 influence the efficiency of the photocatalyst evaluated in HPDW. Inclusion of Ag or Pd enhances the bactericide action of the HPDW system. Ag presents an additional effect besides the photocatalytic one due to its bacteriostatic properties, while Pd enhances the photoactivity due to its electronic properties.

  2. Estudio del equilibrio y cinética de adsorción de Cd(II, Ni(II y Cr(VI usando Quitosano y Quitosano modificado con cobre

    Directory of Open Access Journals (Sweden)

    M. Benavente

    2013-04-01

    Full Text Available En este trabajo, se estudió el equilibrio y la cinética de adsorción de Cd(II, Ni(II en quitosano y Cr(VI en quitosano modificado con cobre. Para ello, soluciones de iones metálicos a diferentes concentraciones, fueron puestas en contacto con el adsorbente y se agitaron por un período de 3 – 4 h. Al final del proceso, las muestras fueron analizadas en un espectrómetro de absorción atómica. Los datos experimentales del equilibrio de adsorción de los iones metálicos fueron evaluados aplicando las isotermas de Langmuir y Freundlich; mientras los datos cinéticos fueron evaluados utilizando los modelos cinéticos de pseudo-primer orden y pseudo-segundo orden. Los resultados del proceso demostraron que los iones fueron eficazmente adsorbidos por el quitosano (Cd y Ni y por el Cu-quitosano (Cr. Además, se comprobó que los datos experimentales del equilibrio de adsorción de Cr(VI y Ni(II se ajustan al modelo de Langmuir; mientras que los datos experimentales del Cd(II fueron mejor ajustados por el modelo de Freundlich. Mediante el uso de la isoterma de Langmuir se determinó la capacidad máxima de adsorción de cromo (29.7 mg/g Cu-quitosano, cadmio (102.0 mg/g quitosano y níquel (83.31 mg/g quitosano. Los resultados de la cinética de adsorción de los iones metálicos mostraron que los datos experimentales fueron mejor ajustados por el modelo de pseudo-segundo orden; es decir, el paso limitante en la velocidad es la reacción de adsorción y no la transferencia de masa.

  3. Determinação do tamanho ótimo da parcela experimental pelos métodos da máxima curvatura modificado, do coeficiente de correlação intraclasse e da análise visual em testes clonais de eucalipto

    Directory of Open Access Journals (Sweden)

    Silva Rogério Luiz da

    2003-01-01

    Full Text Available O objetivo deste trabalho foi determinar o tamanho ótimo da parcela experimental em testes clonais de eucalipto, por meio dos métodos da máxima curvatura modificado, do coeficiente de correlação intraclasse e da análise visual, com base nas características de crescimento em altura, dap e volume. A partir de quatro testes clonais, dispostos no delineamento em blocos ao acaso, com quatro repetições e parcela experimental quadrada de 25 plantas (5 x 5, foram simulados diferentes tamanhos de parcela com 2, 3, 4, 5, 9, 10, 15, 20 e 25 plantas, visando determinar o tamanho ótimo da parcela pelos métodos de máxima curvatura modificado, do coeficiente de correlação intraclasse e da análise visual. Pôde-se concluir que em programas iniciais para seleção de clones parcelas de cinco a dez plantas proporcionam boa precisão experimental, sendo recomendadas, principalmente, em situação com limitações de mudas, teste de grande número de clones e avaliações de cunho preliminar e em idades precoces.

  4. Aplicabilidade da metodologia de reação de polimerase em cadeia em tempo real na determinação do percentual de organismos geneticamente modificados em alimentos Applicability of the real-time polymerase chain reaction based-methods in quantification of genetically modified organisms in foods

    Directory of Open Access Journals (Sweden)

    Natália Eudes Fagundes de Barros

    2008-02-01

    Full Text Available A detecção de organismos geneticamente modificados na cadeia alimentar é um aspecto importante para todos os assuntos envolvidos no controle de matéria-prima, na indústria de alimentos e na distribuição. A rotulagem e a rastreabilidade de organismos geneticamente modificados são questões atuais que são consideradas para o comércio e a regulamentação. Atualmente, a rotulagem de alimentos processados contendo material transgênico detectável é exigida pela legislação brasileira. O governo brasileiro publicou Decreto nº 4.680 em abril de 2003, que exige rotulagem para todos os alimentos ou ingredientes de alimento, com o limite para rotulagem de 1%. Embora a tecnologia de reação em cadeia da polimerase tenha algumas limitações, a alta sensibilidade e especificidade explicam sua escolha por parte dos laboratórios interessados em realizar análises de detecção de organismos geneticamente modificados e seus derivados. Entre os métodos atualmente disponíveis, aqueles baseados na reação em cadeia da polimerase geralmente são aceitos, considerando a sensibilidade e a confiabilidade na detecção de material geneticamente modificado-derivado em análises de rotina. Neste artigo, apresenta-se uma revisão de métodos atualmente disponíveis baseados na reação em cadeia da polimerase para detecção, identificação e quantificação de organismos geneticamente modificados e seus derivados, discutindo sua aplicabilidade e suas limitações.Detection of genetically modified organisms in the food chain is an important issue for all subjects involved in raw material control, food industry and distribution. Both labeling and traceability of genetically modified organisms are current issues that are considered for trade and regulation. Currently, labeling of genetically modified foods containing detectable transgenic material is required by the Brazilian legislation. The Brazilian government published the Decree nº 4.680 in April

  5. Safety and immunogenicity of recombinant poxvirus HIV-1 vaccines in young adults on highly active antiretroviral therapy

    OpenAIRE

    Greenough, Thomas C.; Cunningham, Coleen K; Muresan, Petronella; McManus, Margaret; Persaud, Deborah; Fenton, Terry; Barker, Piers; Gaur, Aditya; Panicali, Dennis; Sullivan, John L.; Luzuriaga, Katherine

    2008-01-01

    A trial to evaluate the safety and immunogenicity of recombinant modified vaccinia Ankara (MVA) and fowlpox (FP) vectors expressing multiple HIV-1 proteins was conducted in twenty HIV-1 infected youth with suppressed viral replication on HAART. The MVA and FP-based multigene HIV-1 vaccines were safe and well tolerated. Increased frequencies of HIV-1 specific CD4+ proliferative responses and cytokine secreting cells were detected following immunization. Increased frequencies and breadth of HIV...

  6. Extended follow-up following a phase 2b randomized trial of the candidate malaria vaccines FP9 ME-TRAP and MVA ME-TRAP among children in Kenya.

    Directory of Open Access Journals (Sweden)

    Philip Bejon

    Full Text Available BACKGROUND: "FFM ME-TRAP" is sequential immunisation with two attenuated poxvirus vectors (FP9 and modified vaccinia virus Ankara delivering the pre-erythrocytic malaria antigen ME-TRAP. Over nine months follow-up in our original study, there was no evidence that FFM ME-TRAP provided protection against malaria. The incidence of malaria was slightly higher in children who received FFM ME-TRAP, but this was not statistically significant (hazard ratio 1.5, 95% CI 1.0-2.3. Although the study was unblinded, another nine months follow-up was planned to monitor the incidence of malaria and other serious adverse events. METHODS AND FINDINGS: 405 children aged 1-6 yrs were initially randomized to vaccination with either FFM ME-TRAP or control (rabies vaccine. 380 children were still available for follow-up after the first nine months. Children were seen weekly and whenever they were unwell for nine months monitoring. The axillary temperature was measured, and blood films taken when febrile. The primary analysis was time to parasitaemia >2,500/microl. During the second nine months monitoring, 49 events met the primary endpoint (febrile malaria with parasites >2,500/microl in the Intention To Treat (ITT group. 23 events occurred among the 189 children in the FFM ME-TRAP group, and 26 among the 194 children in the control group. In the full 18 months of monitoring, there were 63 events in the FFM ME-TRAP group and 60 in the control group (HR = 1.2, CI 0.84-1.73, p = 0.35. There was no evidence that the HR changed over the 18 months (test for interaction between time and vaccination p = 0.11. CONCLUSIONS: Vaccination with FFM ME-TRAP was not protective against malaria in this study. Malaria incidence during 18 months of surveillance was similar in both vaccine groups. TRIAL REGISTRATION: Controlled-Trials.com ISRCTN88335123.

  7. Propriedades do Polestireno Modificado por Nanomateriais

    OpenAIRE

    Oliveira, Pedro Xavier Valente de

    2011-01-01

    A incorporação de nanocargas metálicas em matrizes poliméricas, transformando-as em nanocompósitos, constitui um desafio para a investigação em engenharia, no sentido de se dispor de materiais com melhor desempenho físico/mecânico em serviço. No presente estudo foi efectuada a caracterização de nanocompósitos de matriz polimérica reforçada, primeiro por uma carga metálica nanocristalina e posteriormente com adição de nanotubos. A matriz era poliestireno e os reforços nanocristalinos de aç...

  8. A combined polarizing microscope, XRD, SEM, and specific gravity study of the petrified woods of volcanic origin from the Çamlıdere-Çeltikçi-Güdül fossil forest, in Ankara, Turkey

    Science.gov (United States)

    Hatipoğlu, Murat; Türk, Necdet

    2009-03-01

    The fossil forest in the Çamlıdere-Çeltikçi-Güdül region of the province of Ankara in Turkey has a large number of petrified coniferous and oak tree remains. Petrification occurred in volcanic ashes and tuffs with permineralization, and Fe, Mg, Ca and Ni ions played important roles in the substitution of Si for C. However, the petrified wood samples are heterogeneous in colouration, weight, toughness, and durability, despite being obtained from the same source. Those features are very important for end-users because petrified woods, if cut and polished, are used widely as both decorative indoor tiles and gemstone objects, but heterogeneous materials suffer large wastage while they are being worked and used. Chemical analyses, specific gravity measurements, polarizing microscope studies, X-ray diffraction patterns, and scanning electron image evaluations were performed to classify and identify the homogenous material of the petrified woods relating to its physical and mineralogical characteristics. The different characteristics of the petrified wood samples are due to their varying inner structures, which depend on the replacement silica-building phases and their ratios, and silica particle sizes. Thin sections and XRD patterns revealed that petrified woods in the region were silicified by replacement with both chalcedonic quartz components, including chalcedony (length-fast quartz), moganite and orthorhombic-silica (length-slow quartz), and opalline quartz components including opal-CT and opal-C (length-slow quartz). The scanning electron microscope images were shown that the internal structures of the petrified woods consist of mostly submicron-sized (100-800 nm), and partially nano-sized (60-120 nm) silica-building particles. So, the petrified wood samples can be firstly classified into five main-groups based on their colourations and specific gravity values, then, into three sub-groups based on the principal chalcedonic and opalline quartz silica

  9. High to ultrahigh potassic alkaline volcanic belt along the Ankara-Erzincan suture (northern Turkey): new geochemical and Ar-Ar data constraining petrogenesis with implications for the late Cretaceous subduction of the Neotethys Ocean

    Science.gov (United States)

    Genc, S. Can; Gulmez, Fatma; Tuysuz, Okan; Karacik, Zekiye; Roden, Michael F.; Zeki Billor, M.; Hames, Willis E.

    2013-04-01

    Remnants of some high- to ultrahigh-K alkaline volcanic rocks crop out as isolated small and discontinuous bodies along the Ankara-Erzincan suture belt in northern Turkey. These rocks are represented by leucite-bearing lavas (LB), basaltic andesites, trachytes, monzonite/syenites) and lamprophyres. Leucite-bearing rocks are small stocks, dikes and lava flows. Pebbles and blocks of the LB are found in the coeval volcanic debris avalanche deposits and volcanoclastic breccias. Leucite-bearing rocks are mainly phonotephrite, tephriphonolite, trachyandesite and basaltic trachyandesites (shoshonite) and have mineral assemblages of lct + cpx + ol + pl + Kfs + mag+ ap. Leucites were almost totally analcimized. Trachytes and monzonite/syenites, which are seen as small stocks and dikes, are characterized by amp + bt + pl + Kfs + spn + ap + opq paragenesis. Lamprophyres are mica-rich melanocratic dikes, and include cpx + mica (phlogopitic) + Kfs + ap + opq. Rarely leucite, olivine and plagioclase are also present. Ar-Ar data reveal that this volcanic activity occurred between 73.6±0.18 and 76.78±0.19 Ma, corresponding to latest Cretaceous. All the samples from the high- and ultrahigh-K volcanic belt are alkaline in nature. Leucite-bearing lavas are characterized by their MgO (2.70-5.81, av. 4.58 wt.%), K2O (0.79-4.81, av. 2.35 wt.%), Na2O (4.86-7.48, av. 3.58 wt.%) and K2O/Na2O (0.13-0.92, av. 0.42 wt.%). The low K2O and K2O/Na2O contents of these rocks are due to extensive analcimization of the leucites. Major oxide contents in lamprophyric rocks are 3.25-7.48 (MgO), 1.35-7.76 (K2O), 1.77-4.00 (Na2O) and 0.31-2.69 (K2O/Na2O). The silica content of these rocks are variable and range from 47.18-50.26 (wt.%) (LB) to 39.14-53.28 (lamprophyres). Based on their major element contents, these rocks are classified as plagioleucitites or ultrapotassic rocks of the active orogenic zones (Foley, 1992). Leucite-bearing rocks, lamprophyres and the trachytes (with their hypabyssal

  10. Evaluación de la adherencia de uniones adhesivas metálicas con adhesivos epoxídicos modificados Evaluation of the adherence of bonded metallic joints with modified epoxy adhesives

    Directory of Open Access Journals (Sweden)

    Filiberto González Garcia

    2008-03-01

    Full Text Available En este trabajo fueron evaluadas las propiedades adhesivas de la resina epoxídica del tipo éter diglicidílico del bisfenol A. La resina fue modificada con dos modificadores poliméricos, uno de ellos un copolímero derivado del butadieno, y el otro un copolímero acrílico con el propósito de aumentar la tenacidad de la resina. Tres aminas alifáticas primarias fueron utilizadas como agentes de curado, trietilentetramina, N-(2-aminoetilpiperazina e isoforondiamina. Las propiedades adhesivas fueron investigadas usando la resina pura, así como la resina modificada. Las propiedades adhesivas de la resina modificada y pura fueron estudiadas usando como adherente una aleación de acero (ASTM A36. La adherencia fue evaluada por ensayos de adhesión usando tres geometrías de uniones adhesivas de acero-acero. El comportamiento reológico de los adhesivos fue investigado en condiciones isotérmicas. Los parámetros reológicos relacionados con la reacción de polimerización tales como velocidad de reacción, tiempo de manipulación, y tiempo de gelación de los adhesivos puros fueron relacionados con la estructura química del agente de curado. El tiempo de separación de fases, y de gelación de los adhesivos modificados fue relacionado con la morfología generada, y con la velocidad de la reacción, respectivamente. La morfología fue caracterizada por microscopia electrónica de barrido. La adherencia de las uniones adhesivas sometidas a las diferentes solicitaciones mecánicas fue relacionada a la morfología generada por la fase dispersa de cada modificador, y con las estructuras de redes de los adhesivos.In this work the adhesive properties of epoxy resins based on diglycidyl ether of bisphenol A have been evaluated. The epoxy resin was modified with butadiene and acrylic copolymers to obtain toughened adhesives. The aliphatic primary amines triethylenetetramine, N-(2-aminoethylpiperazine and isophorone diamine were investigated as curing

  11. Ankara Çubuk yöresi turşularından izole edilen laktik asit bakterilerinin tanımlanmaları, teknolojik ve fonksiyonel özelliklerinin belirlenmesi ve starter olarak kullanılma olanaklarının değerlendirilmesi

    OpenAIRE

    TOKATLI, Mehmet

    2013-01-01

      Ankara Çubuk ilçesi turşularından izole edilen laktik asit bakterileri klasik ve moleküler tekniklerle tanımlanmış ve 117 suşun DNA dizi analiz sonucuna göre Lb. brevis (34),  P. ethanolidurans (33), Lb. plantarum (33), Lb. buchneri (9), P. parvulus (3), Lb. namurensis (4),  Lb. diolivarans (1) türlerine ait ol...

  12. Uso de fluorescência em um método de dissector modificado para estimar o número de miócitos no tecido cardíaco Uso de fluorescencia en un método de disector modificado para estimar el número de miocitos en el tejido cardíaco Use of fluorescence in a modified disector method to estimate the number of myocytes in cardiac tissue

    Directory of Open Access Journals (Sweden)

    Rômulo Dias Novaes

    2012-03-01

    Full Text Available FUNDAMENTO: Métodos convencionais de dissector atualmente requerem consideráveis custos financeiros, técnicos e operacionais para estimar o número de células, incluindo cardiomiócitos, em uma área de 3D. OBJETIVO: Usar a microscopia de fluorescência em um método de dissector modificado para determinar o número de miócitos no tecido cardíaco em condições normais e patológicas. MÉTODOS: O estudo empregou camundongos Wistar machos com quatro meses de idade e peso de 366,25 ± 88,21 g randomizados em grupos controles (GC, n = 8 e infectados (GI, n = 8. Os animais do GI foram inoculados com cepa Y de T. cruzi (300.000 tripomastigotas/50 g. Após oito semanas, os animais foram pesados e sacrificados. Os Ventrículos Esquerdos (VE foram removidos para análise estereológica da densidade numérica de cardiomiócitos (Nv [c] e o número total dessas células no VE (N [c]. Esses parâmetros foram estimados usando um dissector fluorescente (DF e comparados com os métodos convencionais de dissector óptico (DO e dissector físico (DFi. RESULTADOS: Em ambos os métodos de dissector, os animais do GI apresentaram queda significativa de Nv[c] e N[c] em comparação com os animais do GC (P > 0,05. Uma correlação forte, igual ou superior a 96%, foi obtida entre DF, DO e DFi. CONCLUSÃO: O método DF parece ser igualmente confiável para determinar Nv[c] e N[c] em condições normais e patológicas, apresentando algumas vantagens em relação aos métodos convencionais de dissector: redução de cortes histológicos e imagens na análise estereológica, redução do tempo de análise das imagens, a construção de DF em microscópios simples, utilizando o modo de epifluorescência, distinção de planos de dissector em ampliações inferiores.FUNDAMENTO: Métodos convencionales de disector actualmente requieren considerables costos financieros, técnicos y operativos para estimar el número de células, incluyendo cardiomiocitos, en un

  13. Caracterização de propriedades funcionais do isolado protéico de sementes de algaroba (Prosopis juliflora(SW D.C.. modificado por acetilação Characterization of functional prorperties of acylated mesquite bean (Prosopis juliflora (SW D.C. protein isolate

    Directory of Open Access Journals (Sweden)

    José Barros da SILVA

    1997-12-01

    Full Text Available O isolado protéico das sementes de algaroba (Prosopis juliflora (SW D.C. foi modificado com anidrido acético nas concentrações de 5, 10, 20 e 30% (V/P, resultando, portanto, nos respectivos graus de modificação: 69,5; 83,2; 89,7 e 91,1% da lisina disponível. Caracterizou-se a funcionalidade do isolado nas formas não modificada e acetilada. O isolado não modificado apresentou alta solubilidade em pH ácido e básico, com exceção da faixa de pH 4 a 6. A acetilação deslocou o ponto isoelétrico das proteínas do pH 5 para o pH 4,5 , diminuiu em pequena extensão a solubilidade abaixo do ponto isoelétrico e aumentou a partir do pH 5, principalmente em pH 7. A capacidade de absorção de água e de óleo do isolado não modificado, que por sua vez se mostrou baixa (1,89 e 1,04g/g proteína, não melhorou satisfatoriamente após a modificação. O efeito de modificação nas propriedades espumantes foi maior com relação ao volume e a expansão da espuma formada do que na sua estabilidade. A capacidade emulsificante do isolado acetilado aumentou em grande extensão, no entanto, a atividade emulsificante e estabilidade de emulsão revelaram pequenos incrementos, em comparação com o isolado não modificado.Protein isolate from seed of mesquite bean (P. juliflora (SW D.C were modified with acetic anhydride at concentrations 5, 10, 20 and 30% (ml per 100 g of protein thus resulting 69.5; 83.2; 89.7 and 91.1% modification of available lysine. Functional characteristic of protein isolate was studied in native and acetilated form. Protein isolate in native form presented high solubility at acidic and basic pH, except in the pH range of 4 to 6. Acetilation shifted the isoelectric point of the proteins pH 5.0 to a pH 4.5; reduced the solubility at pH values below the isoelectric point and increased above pH 5.0, mainly at pH 7.0. The capacity of water and oil absorption of the native protein was small (1.89 and 1.04 g and did not improve

  14. Confiabilidade e validade de um dinamômetro isométrico modificado na avaliação do desempenho muscular em indivíduos com reconstrução do ligamento cruzado anterior Reliability and validity of a modified isometric dynamometer in the assessment of muscular performance in individuals with anterior cruciate ligament reconstruction

    OpenAIRE

    Rodrigo Antunes de Vasconcelos; Débora Bevilaqua-Grossi; Antonio Carlos Shimano; Cleber Jansen Paccola; Tânia Fátima Salvini; Christiane Lanatovits Prado; Mello Junior, Wilson A.

    2009-01-01

    OBJETIVO: Analisar a confiabilidade e validade de um dinamômetro isométrico modificado (DIM) na avaliação dos déficits no desempenho muscular dos extensores e flexores do joelho em indivíduos normais e com reconstrução do LCA. MÉTODOS: Foram convidados 60 voluntários do sexo masculino a participar do estudo, divididos em três grupos de 20 indivíduos: grupo controle (GC), grupo com reconstrução do LCA com tendão patelar (GTP) e grupo com reconstrução do LCA com tendões flexores (GTF). Todos os...

  15. La distribución geográfica de la tángara azul-gris (Thraupis episcopus en hábitats modificados antropogénicamente en México The geographical distribution of the Blue-gray Tanager (Thraupis episcopus through anthropogenically modified habitats in Mexico

    Directory of Open Access Journals (Sweden)

    Erick Rubén Rodríguez-Ruíz

    2011-09-01

    Full Text Available Mediante trabajo de campo, la revisión de bases de datos biológicos e información de la distribución geográfica histórica y actual de Thraupis episcopus se establecieron nuevos registros de anidación en el noreste de México y su ampliación hacia hábitats modificados por actividades humanas en la península de Yucatán y el noreste de México. Además, se evaluó si existía preferencia de la especie por hábitats conservados vs. modificados, por medio de un análisis de escenarios de cambio de uso de suelo de 3 décadas diferentes (1970, 1980 y 1990, obteniendo porcentajes de presencia. Se encontró que no existe una diferencia significativa entre preferencias de hábitat a través del tiempo.We analyzed the historical and current distributional range data of the Blue-gray Tanager, and reported new nesting records in northeastern Mexico. We also assessed the range extension of this species into human-altered habitats in the Yucatán Peninsula and northeastern Mexico, based both on records from fieldwork and biological databases. We identified habitat preference (conserved vs. human altered using land use change scenarios from 3 decades (1970, 1980, and 1990 and percentages of occurrence in a multitemporal approach, finding no significant differences in habitat use through time.

  16. Exosome targeting of tumor antigens expressed by cancer vaccines can improve antigen immunogenicity and therapeutic efficacy.

    Science.gov (United States)

    Rountree, Ryan B; Mandl, Stefanie J; Nachtwey, James M; Dalpozzo, Katie; Do, Lisa; Lombardo, John R; Schoonmaker, Peter L; Brinkmann, Kay; Dirmeier, Ulrike; Laus, Reiner; Delcayre, Alain

    2011-08-01

    MVA-BN-PRO (BN ImmunoTherapeutics) is a candidate immunotherapy product for the treatment of prostate cancer. It encodes 2 tumor-associated antigens, prostate-specific antigen (PSA), and prostatic acid phosphatase (PAP), and is derived from the highly attenuated modified vaccinia Ankara (MVA) virus stock known as MVA-BN. Past work has shown that the immunogenicity of antigens can be improved by targeting their localization to exosomes, which are small, 50- to 100-nm diameter vesicles secreted by most cell types. Exosome targeting is achieved by fusing the antigen to the C1C2 domain of the lactadherin protein. To test whether exosome targeting would improve the immunogenicity of PSA and PAP, 2 additional versions of MVA-BN-PRO were produced, targeting either PSA (MVA-BN-PSA-C1C2) or PAP (MVA-BN-PAP-C1C2) to exosomes, while leaving the second transgene untargeted. Treatment of mice with MVA-BN-PAP-C1C2 led to a striking increase in the immune response against PAP. Anti-PAP antibody titers developed more rapidly and reached levels that were 10- to 100-fold higher than those for mice treated with MVA-BN-PRO. Furthermore, treatment with MVA-BN-PAP-C1C2 increased the frequency of PAP-specific T cells 5-fold compared with mice treated with MVA-BN-PRO. These improvements translated into a greater frequency of tumor rejection in a PAP-expressing solid tumor model. Likewise, treatment with MVA-BN-PSA-C1C2 increased the antigenicity of PSA compared with treatment with MVA-BN-PRO and resulted in a trend of improved antitumor efficacy in a PSA-expressing tumor model. These experiments confirm that targeting antigen localization to exosomes is a viable approach for improving the therapeutic potential of MVA-BN-PRO in humans. PMID:21670078

  17. Comportamento do tebuthiuron em solo de cultivo de cana-de-açúcar utilizando lisímetro de drenagem modificado Tebuthiuron behavior in sugar cane soil using modified drainage lysimeter

    Directory of Open Access Journals (Sweden)

    E.L.C. Souza

    2008-03-01

    Full Text Available O tebuthiuron é um dos herbicidas mais usados no plantio de cana-de-açúcar, no Estado de São Paulo. Estudos têm sido realizados para determinar o índice de lixiviação do tebuthiuron e monitorar sua presença nos mananciais de águas superficiais e subterrâneas, ainda sem uma conclusão definitiva. Com o objetivo de avaliar, em condições de campo, a movimentação vertical do herbicida tebuthiuron em Latossolo Vermelho distroférrico de textura argilosa, testou-se, em ambiente controlado, a hipótese de que o tebuthiuron apresenta baixa mobilidade vertical e pequeno potencial de contaminação de águas subterrâneas. O trabalho foi conduzido no Campo Experimental da Faculdade de Engenharia Agrícola da Unicamp, utilizando-se lisímetro de drenagem modificado de 2 m de diâmetro e 3 m de profundidade, com dez pontos verticais, por meio dos quais foram coletadas amostras de água da chuva. As amostras foram submetidas à Análise Cromatográfica Líquida de Alta Eficiência. Os dados obtidos indicaram presença decrescente do herbicida nas amostras coletadas no período de março a agosto de 2006: amostra 1 - 0,020 g i.a. (5,3%; 2 - 0,016 g i.a. (4,3%; 3 - 0,015 g i.a. (4,0%; 4 - 0,014 g i.a. (3,7%; 5 - 0,014 g i.a. (3,7%; 6 - 0,007 g i.a. (1,9%; 7 - 0,002 g i.a. (0,5%; e 8 - 0,001 g i.a. (0,3% do total aplicado na área do lisímetro (0,3768 g i.a., confirmando a hipótese de baixa mobilidade vertical do tebuthiuron em Latossolo Vermelho distroférrico de textura argilosa, indicando, para esse solo, pequeno potencial de contaminação das águas subterrâneas.Tebuthiuron is one of herbicides most used on sugar cane in the state of São Paulo. Studies have been carried out to determine tebuthiuron leaching index and to monitor its presence in surface and groundwater sources, with no definitive conclusion been reached yet. The aim of this research was to evaluate the vertical movement of tebuthiuron under field conditions on Clayey

  18. Caracterización físico-mecánica, térmica y morfológica de polímeros de redes interpenetradas con base en poliuretano obtenido a partir de aceite de ricino y almidón modificados/polimetilmetacrilato (PMMA) Physico-mechanical, thermal and morphological Behaviour of simultaneous interpenetrating polymer networks based on polyurethane from modified castor oiland starch/poly(Methyl Methacrylate)

    OpenAIRE

    Manuel F. Valero; Jorge E. Pulido; Álvaro Ramírez; Diana C. Camargo; Daniel Navas

    2011-01-01

    El aceite de ricino se modificó por transesterificación con pentaeritritol. Paralelamente, se utilizó la reacción de glucosilación con glicerol para dividir el almidón en unidades monosacáridos. El glucósido obtenido reaccionó por transesterificación con el aceite modificado por transesterificación con pentaeritritol. Se determinó el índice de hidroxilo de los poliol-glucósidos en función del contenido de pentaeritritol y almidón utilizados en la síntesis. Se sintetizaron redes interpenetrada...

  19. Measurements of 222Rn, 220Rn, and CO2 Emissions in Natural CO2 Fields in Wyoming: MVA Techniques for Determining Gas Transport and Caprock Integrity

    Energy Technology Data Exchange (ETDEWEB)

    Kaszuba, John; Sims, Kenneth

    2014-09-30

    An integrated field-laboratory program evaluated the use of radon and CO2 flux measurements to constrain source and timescale of CO2 fluxes in environments proximate to CO2 storage reservoirs. By understanding the type and depth of the gas source, the integrity of a CO2 storage reservoir can be assessed and monitored. The concept is based on correlations of radon and CO2 fluxes observed in volcanic systems. This fundamental research is designed to advance the science of Monitoring, Verification, and Accounting (MVA) and to address the Carbon Storage Program goal of developing and validating technologies to ensure 99 percent storage performance. Graduate and undergraduate students conducted the research under the guidance of the Principal Investigators; in doing so they were provided with training opportunities in skills required for implementing and deploying CCS technologies. Although a final method or “tool” was not developed, significant progress was made. The field program identified issues with measuring radon in environments rich in CO2. Laboratory experiments determined a correction factor to apply to radon measurements made in CO2-bearing environments. The field program also identified issues with radon and CO2-flux measurements in soil gases at a natural CO2 analog. A systematic survey of radon and CO2 flux in soil gases at the LaBarge CO2 Field in Southwest Wyoming indicates that measurements of 222Rn (radon), 220Rn (thoron), and CO2 flux may not be a robust method for monitoring the integrity of a CO2 storage reservoir. The field program was also not able to correlate radon and CO2 flux in the CO2-charged springs of the Thermopolis hydrothermal system. However, this part of the program helped to motivate the aforementioned laboratory experiments that determined

  20. Comparative Assessment of Transmission-Blocking Vaccine Candidates against Plasmodium falciparum

    DEFF Research Database (Denmark)

    Kapulu, M C; Da, D F; Miura, K; Li, Y; Blagborough, A M; Churcher, T S; Nikolaeva, D; Williams, Andrew Richard; Goodman, A L; Sangare, I; Turner, A V; Cottingham, M G; Nicosia, A; Straschil, U; Tsuboi, T; Gilbert, S C; Long, Carole A; Sinden, R E; Draper, S J; Hill, A V S; Cohuet, A; Biswas, S

    2015-01-01

    candidate antigens, have been developed independently and have reported varied transmission-blocking activities (TBA). Here, recombinant chimpanzee adenovirus 63, ChAd63, and modified vaccinia virus Ankara, MVA, expressing AgAPN1, Pfs230-C, Pfs25, and Pfs48/45 were generated. Antibody responses primed...

  1. Effect of one-bottle adhesive systems on the fluoride release of a resin-modified glass ionomer Efeito dos sistemas adesivos de frasco único na liberação de flúor de um cimento de ionômero de vidro modificado por resina

    Directory of Open Access Journals (Sweden)

    Linda Wang

    2004-03-01

    Full Text Available A dhesive systems associated to resin-modified glass ionomer cements are employed for the achievement of a higher bond strength to dentin. Despite this benefit, other properties should not be damaged. This study aimed at evaluating the short-time fluoride release of a resin-modified glass ionomer cement coated with two one-bottle adhesive systems in a pH cycling system. Four combinations were investigated: G1: Vitremer (V; G2: Vitremer + Primer (VP; G3: Vitremer + Single Bond (VSB and G4: Vitremer + Prime & Bond 2.1 (VPB. SB is a fluoride-free and PB is a fluoride-containing system. After preparation of the Vitremer specimens, two coats of the selected adhesive system were carefully applied and light-cured. Specimens were immersed in demineralizing solution for 6 hours followed by immersion in remineralizing solution for 18 hours, totalizing the 15-day cycle. All groups released fluoride in a similar pattern, with a greater release in the beginning and decreasing with time. VP showed the greatest fluoride release, followed by V, with no statistical difference. VSB and VPB released less fluoride compared to V and VP, with statistical difference. Regardless the one-bottle adhesive system, application of coating decreased the fluoride release from the resin-modified glass ionomer cements. This suggests that this combination would reduce the beneficial effect of the restorative material to the walls around the restoration.Sistemas adesivos são associados aos cimentos de ionômero de vidro modificados por resina para a obtenção de maior resistência adesiva à dentina. Apesar deste benefício, outras propriedades não devem ser prejudicadas. Este estudo se propôs a avaliar a liberação de flúor a curto prazo de um cimento de ionômero de vidro modificado por resina coberto com dois diferentes sistemas adesivos em um modelo de ciclagem de pH. Quatro associações foram testadas: G1: Vitremer (V; G2: Vitremer + Primer (VP; G3: Vitremer + Single

  2. Características físico-químicas de amidos modificados com permanganato de potássio/ácido lático e hipoclorito de sódio/ácido lático Physicochemical characteristics of starches modified with potassium permanganate/lactic acid and sodium hypochlorite/lactic acid

    Directory of Open Access Journals (Sweden)

    Roberto Marques Silva

    2008-03-01

    Full Text Available Amidos oxidados em elevadas concentrações produzem pastas fluidas, são estáveis à retrogradação e aplicados em indústrias de papel, têxtil e de alimentos. Esta propriedade é atribuída à presença de carboxilas, com cargas negativas e mais volumosas que as hidroxilas. Neste trabalho, amidos de batata, batata doce, mandioca, mandioquinha salsa, milho e milho ceroso foram modificados com KMnO4/ácido lático e NaOCl/ácido lático e caracterizados quanto ao teor de carboxilas, poder redutor, coloração diferencial, propriedade de expansão e propriedades viscoamilográficas, em água e tampões acetato (pH 4,0 e fosfato (pH 7,0. Amidos de milho ceroso e de mandioca modificados com KMnO4/ácido lático apresentaram elevadas expansões (25,8 e 24,1 mL.g-1, respectivamente. As pastas de amido de milho ceroso resistiram a ciclos de congelamento/descongelamento. A coloração diferencial e o teor de carboxilas não permitiram diferenciar as amostras; apenas o amido de batata reagiu com azul de metileno devido aos grupos fosfato. Quanto ao pH e acidez, as amostras apresentaram valores próximos da neutralidade, exceto o amido de milho e milho ceroso modificados com NaOCl. Amidos oxidados com KMnO4/ácido lático apresentaram picos de viscosidade inferiores aos tratados com NaOCl. Os picos de viscosidade em tampão fosfato foram inferiores aos em água e em tampão acetato.Oxidized starches produce low-viscosity pastes even in high concentrations, are stable to retrogradation and are used in the paper, textile and food industries. This property is attributed to the presence of carboxyl groups, which have negative charges and are bulkier than hydroxyls. In this work, potato, sweet potato, Peruvian carrot, cassava, corn and waxy corn starches were modified with oxidative reagents and characterized with respect to their carboxyl content, reducing power, differential dyeing, expansion power and viscographic properties, in water, acetate buffer (p

  3. Efeito de sêmen resfriado e diluído em Beltsville Thawing Solution, Zorlesco-modificado e BTZOR no desempenho reprodutivo de fêmeas suínas inseminadas Reproductive performance of swine females inseminated with cooled and diluted semen In Beltsville Thawing Solution, modified-Zorlesco and BTZOR

    Directory of Open Access Journals (Sweden)

    Ana Maria Martins Alves Vasconcelos

    2001-04-01

    Full Text Available Utilizaram-se 83 fêmeas, inseminadas com ejaculados de três cachaços, diluídos em Beltsville Thawing Solution (BTS, Zorlesco-modificado (ZOR e BTZOR (meio desenvolvido na Universidade Estadual de Maringá, sendo 28 fêmeas inseminadas com o diluente BTS, 28 com ZOR e 27 com BTZOR, com o objetivo de estudar os índices de retorno ao cio e de parição e os números médios de leitões nascidos e nascidos vivos das fêmeas inseminadas. As fêmeas inseminadas com ZOR apresentaram melhor índice de parição (92,9%, quando comparadas às inseminadas com BTZOR (70,4% e BTS (67,9%. O índice de retorno ao cio foi menor nas fêmeas inseminadas com ZOR (7,14% em comparação às inseminadas com BTZOR (29,6% e BTS (32,14%, não tendo diferença entre o BTS e o BTZOR. Concluiu-se que os melhores resultados quanto ao desempenho reprodutivo das fêmeas ocorreram nas inseminações realizadas com sêmen diluído em Zorlesco-modificado.Eighty-three swine females were inseminated with semen from three boars, diluted in Beltsville Thawing Solution (BTS, Modified-Zorlesco (ZOR and BTZOR (a medium developed at Universidade Estadual de Maringá. Twenty eight females were inseminated with BTS, 28 with ZOR and 27 with BTZOR, with the objective to evaluate the indexes of heat return and farrowing rate, and the averages numbers of born piglets and born alive piglets from the inseminated females. The females inseminated with ZOR showed the best farrowing rate index (92.9% when compared to those inseminated with BTZOR (70,4% and with BTS (67.9%. The lowest heat return rate was from inseminated females with ZOR (7.14% when compared to BTZOR (29.6% and BTS (32.14%, and there was no difference between BTS and BTZOR. The best reproductive performance results were observed for artificial inseminations with semen diluted in Modified-Zorlesco diluents.

  4. Métodos de coloração de Roeser (1972: modificado - e Kropp (1972 visando a substituição do azul de astra por azul de alcião 8GS ou 8GX Staining methods of modified Roeser (1972 and Kropp (1972, aiming at substituing the astra blue by alcian blue 8GS or 8GX

    Directory of Open Access Journals (Sweden)

    Rebeca Luque

    1996-12-01

    Full Text Available Em folhas de Lychnophora ericoides, Coffea arabica e Nymphaea mexicana foram testados métodos de coloração baseados em Roeser (1972 modificado e Kropp (1972, visando a substituição do corante azul de astra por azul de alcião 8GS ou 8GX. As amostras foram fixadas em FAA, desidratadas em série butílica terciária e incluídas em parafina. Os cortes histológicos transversais foram corados segundo diferentes baterias de coloração, modificadas quanto ao tipo de corante usado, diferenciador e série de desidratação. As lâminas permanentes foram preparadas com bálsamo-do-canadá sintético. Os resultados obtidos evidenciaram que o método de Roeser (1972 modificado é melhor que o de Kropp (1972, nas condições deste experimento. O azul de astra pode ser substituído por azul de alcião 8GX e a desidratação pode ser em série isopropílica ou etílica, sem grande diferença entre elas. São discutidos os resultados provenientes das diferentes colorações.Staining methods based on modified Roeser (1972 as well as that of Kropp (1972 were done with leaves of Lychnophora ericoides, Coffea arabica and Nymphaea mexicana aiming at substituing the astra blue stain by alcian blue 8GS or 8GX. Samples were fixed in FAA, dehydrated in teit-butyl alcohol series and embedded in paraffin. The histological transversal sections were stained in different staining batteries, modified according to the type of stains and differentiators, and the series of dehydration. Permanent slides were prepared with synthetic Canada balsam. The results obtained indicate that the modified Roeser method (1972 is better than that of Kropp (1972 under present experimental conditions. The astra blue can be substituted by alcian blue 8GX and dehydration can be isopropylic or ethylic, with little difference between them. The results obtained with the different staining techniques used are discussed.

  5. Infrared-spectroscopy analysis of zinc phosphate and nickel and manganese modified zinc phosphate coatings on electrogalvanized steel Análise por espectroscopia no infravermelho das camadas de fosfato de zinco e de zinco modificado com níquel e manganês em aço eletrogalvanizado

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    Kirlene Salgado Fernandes

    2011-03-01

    Full Text Available Hopeite-type phosphate coatings in which zinc is partially replaced by other metals like manganese and nickel are of great interest for the automotive and home appliance industries. Such industries use phosphate conversion coatings on galvanized steels in association with cataphoretic electropainting. Zinc phosphates modified with manganese and nickel are isomorphic with the hopeite, and the phase identification using X-ray diffraction is difficult. In this paper, the phosphate coatings are identified using the Fourier transform infrared spectroscopy (FTIR.As camadas de fosfato do tipo hopeíta, nas quais o zinco é parcialmente substituído por outros metais, como manganês e níquel, são de grande interesse para a indústria automotiva e de eletrodomésticos. Essas indústrias utilizam aços galvanizados, fosfatizados e pintados com eletropintura cataforética. Os fosfatos de zinco modificados com manganês e níquel são isomorfos com a hopeíta, e de difícil identificação usando-se a técnica de difração de raios X. Nesse trabalho, as camadas de conversão de fosfato foram identificadas usando-se a espectroscopia no infravermelho por transformada de Fourier (FTIR.

  6. A problemática dos organismos geneticamente modificados e a formação científica do cidadão comum: um estudo com manuais escolares de Ciências Naturais do 9º ano adotados em Portugal

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    Luís Dourado

    2014-12-01

    Full Text Available Uma das aplicações da biotecnologia são os organismos geneticamente modificados (OGM, os quais têm uma utilização cada vez maior, apresentando vantagens, mas, também, sendo referidos como organismos potencialmente perigosos. Correspondendo a esses factos, os manuais de ciências dedicam algumas das suas páginas à biotecnologia, sendo os OGMs um dos aspetos focados. Nesse sentido e considerando a importância que o manual escolar tem na educação em ciências, procedemos a um estudo que envolveu a análise dos manuais escolares de ciências naturais do 9º ano adotados em Portugal, na parte respeitante aos OGMs. Neste estudo verificou-se que os manuais nem sempre apresentam a informação correta e necessária, de forma adequada, e que nem sempre lhe dão o devido destaque. Dado que a nossa investigação revela a existência de falhas ao nível dos manuais, as conclusões obtidas podem contribuir para uma melhoria dos mesmos e, consequentemente, da qualidade da educação em ciências.

  7. Ns1 is a key protein in the vaccine composition to protect Ifnar(-/- mice against infection with multiple serotypes of African horse sickness virus.

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    Francisco de la Poza

    Full Text Available African horse sickness virus (AHSV belongs to the genus Orbivirus. We have now engineered naked DNAs and recombinant modified vaccinia virus Ankara (rMVA expressing VP2 and NS1 proteins from AHSV-4. IFNAR((-/- mice inoculated with DNA/rMVA-VP2,-NS1 from AHSV-4 in an heterologous prime-boost vaccination strategy generated significant levels of neutralizing antibodies specific of AHSV-4. In addition, vaccination stimulated specific T cell responses against the virus. The vaccine elicited partial protection against an homologous AHSV-4 infection and induced cross-protection against the heterologous AHSV-9. Similarly, IFNAR((-/- mice vaccinated with an homologous prime-boost strategy with rMVA-VP2-NS1 from AHSV-4 developed neutralizing antibodies and protective immunity against AHSV-4. Furthermore, the levels of immunity were very high since none of vaccinated animals presented viraemia when they were challenged against the homologous AHSV-4 and very low levels when they were challenged against the heterologous virus AHSV-9. These data suggest that the immunization with rMVA/rMVA was more efficient in protection against a virulent challenge with AHSV-4 and both strategies, DNA/rMVA and rMVA/rMVA, protected against the infection with AHSV-9. The inclusion of the protein NS1 in the vaccine formulations targeting AHSV generates promising multiserotype vaccines.

  8. Uso do índice de Kotelchuck modificado na avaliação da assistência pré-natal e sua relação com as características maternas e o peso do recém-nascido no Município do Rio de Janeiro Use of the modified Kotelchuck index in the evaluation of prenatal care and its relationship to maternal characteristics and birth weight in Rio de Janeiro, Brazil

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    Maria do Carmo Leal

    2004-01-01

    Full Text Available O índice de Kotelchuck (IK foi modificado e utilizado para avaliar a assistência pré-natal prestada no Município do Rio de Janeiro - Brasil, em uma amostra de 9920 puérperas de parto único. Regressões multivariadas logística ordinal (RMLO e linear (RML foram utilizadas para estimar a importância de fatores demográficos, psicossociais e obstétricos no IK modificado e seus efeitos no peso ao nascer (PN, respectivamente. Apenas 38,5% das parturientes do Município do Rio de Janeiro foram classificadas como de cuidado pré-natal adequado ou intensivo. Após ajustamento por outros preditores, mantiveram-se como variáveis explicativas do IK: o nível de instrução, viver com o pai do RN, tentar abortar, diabetes, satisfação com a gravidez, cor da pele, paridade, idade e local de residência. O PN associou-se com o IK modificado, mesmo após o controle de variáveis sociodemográficas, comportamentais e biológicas. A utilização adequada da assistência pré-natal no Município do Rio de Janeiro contribuiu na prevenção do PN e as mães que menos utilizaram os serviços pré-natais têm piores condições socioeducacionais, de apoio familiar e de risco obstétrico.The Kotelchuck index (KI was modified and used to evaluate prenatal care provided in the City of Rio de Janeiro, Brazil, in a sample of 9,920 post-partum women following singleton deliveries. Ordinal logistic regression (OLR and multivariate linear regression (LMR were used to estimate the importance of demographic, psychosocial, and obstetric factors for modified KI and the effects on birth weight (BW, respectively. Only 38.5% of the sample was classified as having received adequate or intensive prenatal care. After adjusting for other predictors, the explanatory variables for KI were: mother's schooling, living with the newborn's father, attempted abortion, diabetes mellitus, satisfaction with pregnancy, skin color, parity, age, and place of residence. BW was associated

  9. Estudo da validade e confiabilidade intra e interobservador da versão modificada do teste de Schöber modificado em indivíduos com lombalgia Study of validity and intra and inter-observer reliability of modified-modified Schöber test in subjects with low-back pain

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    Christiane de Souza Guerino Macedo

    2009-09-01

    Full Text Available Em pacientes com lombalgia, mensura-se a amplitude de movimento (ADM da coluna lombar por meio da versão modificada do teste de Schöber modificado (MTSM, mas suas propriedades psicométricas não são comprovadas para uso clínico. Este estudo verificou a validade e confiabilidade intra e interobservador do MTSM em indivíduos com lombalgia, comparando as medidas da ADM com as obtidas por meio de radiografia, método considerado padrão-ouro. Participaram 20 voluntários com lombalgia, de ambos os sexos, funcionários de um Hospital Universitário. O MTSM foi aplicado duas vezes por dois avaliadores. As medidas obtidas pelo teste e por radiografia foram comparadas usando o coeficiente de correlação de Pearson, obtendo-se r=0,14, ou seja, correlação fraca. O coeficiente de correlação intraclasse (CCI dos MTSM intra-observador foi 0,96 (IC 95% 0,91;0,98 e interobservador 0,93 (IC 95% 0,84;0,97, indicando alta confiabilidade; o teste de Bland & Altman mostrou alta concordância intra e interobservador, com valores de -0,21 e -0,28, respectivamente. Embora tenha sido encontrada alta confiabilidade intra e interobservador na aplicação da versão modificada do teste de Schöber modificado, este apresentou baixa validade para medir a ADM da coluna lombar, quando comparado ao padrão-ouro.In patients with low-back pain the lumbar spine range of motion (ROM is often measured by the modified version of the modified Schöber test (MMST, but its psychometric properties have not been ascertained for clinical use. The purpose here was to verify intra and inter-observer validity and reliability of the MMST in subjects with low-back pain, and to compare obtained ROM measures to those obtained by radiography, taken as gold standard. The study involved 20 subjects with chronic low-back pain, of both sexes, employees at a university hospital. The MMST was applied twice by two examiners each. The Pearson correlation coefficient found when comparing

  10. Reproducibility of maximum aerobic power (VO2max among soccer players using a modified heck protocol Reprodutibilidade da potência aeróbia máxima (VO2max em jogadores de futebol utilizando o protocolo de heck modificado

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    Paulo Roberto Santos-Silva

    2007-01-01

    Full Text Available OBJECTIVE: To determine the degree of reproducibility of maximum oxygen consumption (VO2max among soccer players, using a modified Heck protocol. METHODS: 2 evaluations with an interval of 15 days between them were performed on 11 male soccer players. All the players were at a high performance level; they were training for an average of 10 hours per week, totaling 5 times a week. When they were evaluated, they were in the middle of the competitive season, playing 1 match per week. The soccer players were evaluated on an ergometric treadmill with velocity increments of 1.2 km.h-1 every 2 minutes and a fixed inclination of 3% during the test. VO2max was measured directly using a breath-by-breath metabolic gas analyzer. RESULTS: The maximum running speed and VO2max attained in the 2 tests were, respectively: (15.6 ± 1.1 vs. 15.7 ± 1.2 km.h-1; [P = .78] and (54.5 ± 3.9 vs. 55.2 ± 4.4 ml.kg-1.min-1; [P = .88]. There was high and significant correlation of VO2max between the 2 tests with a 15-day interval between them [r = 0.97; P OBJETIVO: Determinar o grau de reprodutibilidade do consumo máximo de oxigênio (VO2max em jogadores de futebol utilizando o protocolo de Heck modificado. MÉTODOS: Foram avaliados por duas vezes, com intervalo de 15 dias entre os testes, 11 futebolistas masculinos. Todos eram de alto nível, treinavam em média 10 horas por semana subdivididos em treinamentos físicos, técnicos, táticos e jogos competitivos, totalizando cinco vezes por semana e na fase em que foram avaliados se encontravam em pleno período competitivo realizando um jogo por semana. Os futebolistas foram avaliados em esteira ergométrica (1,2 km.h-1 a cada dois minutos e inclinação fixa durante o teste em 3%. O VO2max foi medido diretamente utilizando analisador metabólico de análise de gases expirados respiração-a-respiração. RESULTADOS: A velocidade máxima de corrida e o VO2max atingido nos dois testes foram respectivamente: (15,6 ± 1

  11. Caracterización físico-mecánica, térmica y morfológica de polímeros de redes interpenetradas con base en poliuretano obtenido a partir de aceite de ricino y almidón modificados/polimetilmetacrilato (PMMA

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    Manuel F. Valero

    2011-01-01

    Full Text Available El aceite de ricino se modificó por transesterificación con pentaeritritol. Paralelamente, se utilizó la reacción de glucosilación con glicerol para dividir el almidón en unidades monosacáridos. El glucósido obtenido reaccionó por transesterificación con el aceite modificado por transesterificación con pentaeritritol. Se determinó el índice de hidroxilo de los poliol-glucósidos en función del contenido de pentaeritritol y almidón utilizados en la síntesis. Se sintetizaron redes interpenetradas de polímero de manera simultánea (SINs con diferentes relaciones en peso poliuretano/PMMA de 100/0, 90/10, 80/20, 70/30, 60/40 y 50/50. Para la síntesis de la red de poliuretano (PU se utilizaron los poliol-glucósidos y diisocianato de isoforona (IPDI o con una relación NCO/OH = 1 y para la síntesis de la red de polimetilmetacrilato (PMMA se utilizó peróxido de benzoílo como agente iniciador y dimetacrilato de etilenglicol como agente entrecruzante. Los SINs se caracterizaron por sus propiedades fisicomecánicas como resistencia al ataque químico, dureza, resistencia a la tensión y elongación de ruptura. La estabilidad térmica de los SINs se determinó usando análisis termogravimétrico (TGA. La morfología de superficie se determinó usando microscopia electrónica de barrido (SEM y mostró una morfología de dos fases para todos los SINs.

  12. Ankara’daki Üniversite ve Halk Kütüphanelerinde Çalışan Kütüphanecilerin İş Doyumları Üzerine Bir Araştırma =A Research on Job Satisfaction of Librarians Employed at University and Public Libraries in Ankara

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    Yılmaz, Bülent

    2010-04-01

    Full Text Available Kişinin yaptığı işten hoşnut olması anlamına gelen iş doyumu genelde ve kütüphanecilik alanı açısından işteki başarı ve verimliliği etkileyen, aynı zamanda insanın yaşamdan doyum almasına önemli katkıda bulunan çok boyutlu bir olgudur. Bu çalışmada, öncelikle iş doyumu hakkında genel ve kütüphanecilik temelli kuramsal bilgi verilmiştir. Daha sonra yapılan araştırma sonuçları değerlendirilmiş ve konu ile ilgili öneriler sunulmuştur. Bu çalışmaya konu olan araştırma Ankara'da bulunan üniversite ve halk kütüphanelerinde çalışan kütüphaneciler üzerinde gerçekleştirilmiştir. Ankara'daki on üniversite kütüphanesinden 69, altı halk kütüphanesinden 20 olmak üzere toplam 89 kütüphaneciye Mayıs 2009 tarihinde anket uygulanmıştır. Anket için Spector'un "İş Doyumu Ölçeği" temel alınmıştır. Araştırmada elde edilen sonuçlara göre, üniversite ve halk kütüphanelerinde çalışan kütüphanecilerin genelde iş doyumları arasında istatistiksel olarak anlamlı bir fark bulunmadığı, ancak, bazı unsurlar arasında anlamlı farklılıklar olduğu saptanmıştır.Job satisfaction, which signifi es an individual's happiness in his/her occupation, is a multidimensional case that aff ects success and productivity at work, in general terms and in the fi eld of librarianship. This article off ers primarily general information on job satisfaction and librarianship-based theoretical information. It also evaluates the conclusions of a questionnaire and off ers related suggestions. The research focused on the librarians employed at university and public libraries in Ankara. A questionnaire was administered in May 2009 to 69 university librarians and 20 others employed by public libraries, bringing the total to 89 professional men and women. The questionnaire has been based on the Spector's "Job Satisfaction Survey". According to the conclusions that may be derived from the research

  13. Poli(Álcool Vinílico Modificado com Cadeias Hidrocarbônicas: Avaliação do Balanço Hidrófilo/Lipófilo Chemical Modification of Poly(Vinyl Alcohol: Evaluation of Hydrophilic/Lipophilic Balance

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    Isabele B. Aranha

    2001-01-01

    Full Text Available Terpolímeros de poli(álcool vinílico foram sintetizados por meio de reação de esterificação do PVA parcialmente hidrolisado com cloretos de ácidos graxos de comprimento de cadeia diferentes. O objetivo da síntese foi obter polímeros à base de PVA com pequenas variações no balanço hidrófilo/lipófilo e nas propriedades interfaciais de suas soluções. A modificação foi acompanhada por ressonância magnética nuclear de hidrogênio e as propriedades foram avaliadas pela variação na solubilidade e na tensão superficial. Foram obtidos polímeros modificados com baixos teores de grupamento hidrófobo. Testes de tensão superficial, realizados com os produtos solúveis em água, não mostraram variação dessa propriedade. A solubilidade dos produtos diminuiu sensivelmente mesmo para pequenas incorporações de grupamento hidrófobo. Foi observado que, para uma mesma massa, segmentos hidrófobos menores, distribuídos ao longo da cadeia, promovem maior modificação na solubilidade.Poly(vinyl alcohol terpolymers have been obtained by reaction of partially hydrolized poly(vinyl alcohol with different acid chlorides. The objective is the preparation of polymers with slight differences in their hydrophilic/lipophilic balance and in the interfacial activities of their solutions. The chemical modifications were characterized by means of ¹H NMR and the polymer properties were evaluated in terms of changes in solubility and surface tension. By chemical modification, polymers with low percentage of hydrophobic group were obtained. The water-soluble polymers obtained did not have the surface tension of their solutions altered. The solubility of the modified polymers decreased markedly, even with low contents of hydrophobic groups.

  14. Nuevo método gravimétrico, esencialmente modificado, para la determinación de sílice en silicatos atacables por ácido, basado en los nuevos conocimientos descubiertos en el estudio del comportamiento de los geles de sílice deshidratados en soluciones ácid

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    Goma, F.

    1976-12-01

    Full Text Available Not availableSe investigan, en primer lugar, las causas de error en la determinación de sílice que provienen de las condiciones externas al método en s% como son: la homogeneización, el estado de la muestra en el momento de la pesada para el análisis, y la influencia que tiene el contenido de los álcalis, parcialmente volátiles, sobre la determinación de la cantidad de materia fija después de la calcinación. Se establecen las condiciones previas del tratamiento de la muestra, según su naturaleza, para obtener siempre resultados reproducibles. Un estudio sistemático del comportamiento de los geles de sílice, obtenidos en las mismas condiciones que los que se producen en el análisis, ha permitido explicar, por primera vez, las causas que producen las llamadas "segundas sílices" y permite ver que el paso de sílice a la solución es debido a una disolución verdadera y no a un fenómeno coloidal. La aplicación al procedimiento clásico, de todos estos hechos experimentales hallados en estas investigaciones, ha permitido establecer un método simplificado y esencialmente modificado, que se describe. Se compara su alcance con los actuales métodos de mayor autoridad y se concluye que, con una sola extracción, se consigue: una recuperación prácticamente total de sílice y conocer la cantidad ''mínima y constante" de sílice que pasa al filtrado, con lo que se ha conseguido, además de la simplificación del método, un mayor grado de exactitud y un margen de reproducibilidad que es más estrecho en cualquier caso y cuando la sílice es componente mayoritario, como en los clínkeres y cementos portland, se estima es ± 0,05.

  15. Los alimentos modificados. ??El omn??voro desculturalizado?

    OpenAIRE

    Contreras, Jes??s; Ribas, Joan

    2012-01-01

    Los avances cient??ficos y la innovaci??n tecnol??gica en el sector agroalimentario han dado lugar a una ruptura fundamental de las relaciones que los seres humanos hab??an mantenido f??sicamente con su medio. La 'industrializaci??n', percibida en gran medida como una 'artificializaci??n' de la alimentaci??n, ha dado lugar a la idea de que cada vez sabemos menos acerca de lo que comemos, a pesar de que nunca como hoy hab??amos tenido tantos conocimientos sobre los alimentos, las enfermedades...

  16. A Viral Vectored Prime-Boost Immunization Regime Targeting the Malaria Pfs25 Antigen Induces Transmission-Blocking Activity

    OpenAIRE

    Goodman, Anna L.; Blagborough, Andrew M.; Sumi Biswas; Yimin Wu; Hill, Adrian V.; Sinden, Robert E.; Draper, Simon J

    2011-01-01

    The ookinete surface protein Pfs25 is a macrogamete-to-ookinete/ookinete stage antigen of Plasmodium falciparum, capable of exerting high-level anti-malarial transmission-blocking activity following immunization with recombinant protein-in-adjuvant formulations. Here, this antigen was expressed in recombinant chimpanzee adenovirus 63 (ChAd63), human adenovirus serotype 5 (AdHu5) and modified vaccinia virus Ankara (MVA) viral vectored vaccines. Two immunizations were administered to mice in a ...

  17. Differential CD4+ versus CD8+ T-Cell Responses Elicited by Different Poxvirus-Based Human Immunodeficiency Virus Type 1 Vaccine Candidates Provide Comparable Efficacies in Primates▿ †

    OpenAIRE

    Mooij, Petra; Balla-Jhagjhoorsingh, Sunita S.; Koopman, Gerrit; Beenhakker, Niels; van Haaften, Patricia; Baak, Ilona; Nieuwenhuis, Ivonne G.; Kondova, Ivanela; Wagner, Ralf; Wolf, Hans; Gómez, Carmen E.; José L Nájera; Jiménez, Victoria; Esteban, Mariano; Heeney, Jonathan L.

    2008-01-01

    Poxvirus vectors have proven to be highly effective for boosting immune responses in diverse vaccine settings. Recent reports reveal marked differences in the gene expression of human dendritic cells infected with two leading poxvirus-based human immunodeficiency virus (HIV) vaccine candidates, New York vaccinia virus (NYVAC) and modified vaccinia virus Ankara (MVA). To understand how complex genomic changes in these two vaccine vectors translate into antigen-specific systemic immune response...

  18. 8MVA modulator/regulator for neutral beams

    International Nuclear Information System (INIS)

    This paper describes very generally the modulator/regulator (Mod/Reg) being built for Transrex by Systems, Science and Software for use on the neutral beam power supplies that Transrex is building for General Atomic Company to power the neutral beam heating systems that will be used on the Doublet III fusion device. The Mod/Reg is required to provide an 80 kV, 100 A pulse for a second every 90 sec. The voltage is to be regulated to 3%, and in case of fault the pulse must be interrupted within 10 μsec. An additional requirement was that the total system have very low capacity such that the total energy stored would be less than 15 joules. This is a restriction imposed by the source designer to prevent destroying the source in case of an arc within the source

  19. The Future of Smallpox Vaccination: is MVA the key?

    OpenAIRE

    Slifka, Mark K.

    2005-01-01

    Eradication of the smallpox virus through extensive global vaccination efforts has resulted in one of the most important breakthroughs in medical history, saving countless lives from the severe morbidity and mortality that is associated with this disease. Although smallpox is now extinct in nature, laboratory stocks of this virus still remain and the subject of smallpox vaccination has gained renewed attention due to the potential risk that smallpox may be used as a biological weapon by terro...

  20. Attenuated recombinant vaccinia virus expressing oncofetal antigen (tumor-associated antigen) 5T4 induces active therapy of established tumors.

    Science.gov (United States)

    Mulryan, Kate; Ryan, Matthew G; Myers, Kevin A; Shaw, David; Wang, Who; Kingsman, Susan M; Stern, Peter L; Carroll, Miles W

    2002-10-01

    The human oncofetal antigen 5T4 (h5T4) is a transmembrane glycoprotein overexpressed by a wide spectrum of cancers, including colorectal, ovarian, and gastric, but with a limited normal tissue expression. Such properties make 5T4 an excellent putative target for cancer immunotherapy. The murine homologue of 5T4 (m5T4) has been cloned and characterized, which allows for the evaluation of immune intervention strategies in "self-antigen" in vivo tumor models. We have constructed recombinant vaccinia viruses based on the highly attenuated and modified vaccinia virus ankara (MVA strain), expressing h5T4 (MVA-h5T4), m5T4 (MVA-m5T4), and Escherichia coli LacZ (MVA-LacZ). Immunization of BALB/c and C57BL/6 mice with MVA-h5T4 and MVA-m5T4 constructs induced antibody responses to human and mouse 5T4, respectively. C57BL/6 and BALB/c mice vaccinated with MVA-h5T4 were challenged with syngeneic tumor line transfectants, B16 melanoma, and CT26 colorectal cells that express h5T4. MVA-h5T4-vaccinated mice showed significant tumor retardation compared with mice vaccinated with MVA-LacZ or PBS. In active treatment studies, inoculation with MVA-h5T4 was able to treat established CT26-h5T4 lung tumor and to a lesser extent B16.h5T4 s.c. tumors. Additionally, when C57BL/6 mice vaccinated with MVA-m5T4 were challenged with B16 cells expressing m5T4, resulting growth of the tumors was significantly retarded compared with control animals. Furthermore, mice vaccinated with MVA-m5T4 showed no signs of autoimmune toxicity. These data support the use of MVA-5T4 for tumor immunotherapy. PMID:12481437

  1. Confiabilidade e validade de um dinamômetro isométrico modificado na avaliação do desempenho muscular em indivíduos com reconstrução do ligamento cruzado anterior Reliability and validity of a modified isometric dynamometer in the assessment of muscular performance in individuals with anterior cruciate ligament reconstruction

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    Rodrigo Antunes de Vasconcelos

    2009-06-01

    Full Text Available OBJETIVO: Analisar a confiabilidade e validade de um dinamômetro isométrico modificado (DIM na avaliação dos déficits no desempenho muscular dos extensores e flexores do joelho em indivíduos normais e com reconstrução do LCA. MÉTODOS: Foram convidados 60 voluntários do sexo masculino a participar do estudo, divididos em três grupos de 20 indivíduos: grupo controle (GC, grupo com reconstrução do LCA com tendão patelar (GTP e grupo com reconstrução do LCA com tendões flexores (GTF. Todos os indivíduos realizaram teste isométrico dos extensores e flexores do joelho no DIM; os déficits de força muscular coletados foram comparados posteriormente com os testes realizados no Biodex System 3 operando no modo isométrico e isocinético nas velocidades de 60º/s e 180º /s. Foram realizados cálculos de correlação intraclasse ICC para avaliar a confiabilidade do DIM, cálculos da especificidade, sensibilidade e coeficiente de concordância Kappa, respectivamente, para avaliar a validade do DIM em detectar déficits musculares e comparações intragrupos e intergrupos na realização dos quatro testes de força utilizando-se do método ANOVA. RESULTADOS: O DIM demonstrou excelente confiabilidade teste-reteste e validade na avaliação do desempenho muscular dos extensores e flexores do joelho. Na comparação intergrupos. o GTP demonstrou déficits significativamente maiores dos extensores comparados com os grupos GC e GTF. CONCLUSÃO: Dinamômetros isométricos conectados em equipamentos de mecanoterapia podem ser uma alternativa para coletar dados referentes a déficits no desempenho muscular dos extensores e flexores do joelho em indivíduos com reconstrução do LCA.OBJECTIVES: The aim of this study was to evaluate the reliability and validity of a modified isometric dynamometer (MID in performance deficits of the knee extensor and flexor muscles in normal individuals and in those with ACL reconstructions. METHODS: Sixty male

  2. Cementos óseos acrílicos modificados con hidroxiapatita/acetato de vinilo: caracterización mecánica, termoanálitica y bioactividad in vitro Acrylic bone cement modified whit hydroxiapatyte/vinyl acetate: mechanical, thermoanalytical characterization and in vitro bioactivity

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    Nayrim B. Guerra

    2010-06-01

    Full Text Available Los cementos óseos se han convertido en los últimos años en biomateriales de gran utilidad en la fijación de prótesis y en la reconstrucción del hueso. El objetivo de este trabajo es evaluar las propiedades termoanalíticas tales como temperatura máxima de polimerización y tiempo de fraguado en cementos óseos acrílicos modificados con hidroxiapatita/acetato de vinilo, determinar la resistencia a la compresión axial y realizar ensayos de bioactividad in vitro. Diferentes contenidos de acetato de vinilo fueron incorporados en cementos óseos acrílicos cargados todos con un 30 % de hidroxiapatita CORALINA® HAP-200. Las propiedades mecánicas y los parámetros de curado fueron evaluados cumpliendo lo establecido en la Norma ISO 5833 descrita para cementos óseos acrílicos. Se determinaron los parámetros termoanalíticos, obteniéndose tiempos de fraguados entre 3 y 6 minutos y los valores de temperaturas máximas de polimerización oscilan entre 66 y 88 °C. Se obtuvo formulaciones con valores de resistencia a la compresión superiores a lo establecido en la Norma ISO 5833. Se demostró la bioactividad de las formulaciones mediante la inmersión de las muestras en fluido biológico simulado, observándose en la superficie de las mismas la nucleación y el crecimiento de cristales con morfología similar a las apatitas biológicas.Bone cements have become biomaterials of great utility in the prosthesis fixation and as substitutes to the bone. The objective of this study is to evaluate the thermo analytical properties such as setting time and peak temperature of polymerization of acrylic bone cement modified with hydroxyapatite/vinyl acetate, to determine the compression strengths and perform in vitro bioactivity tests. Amounts of vinyl acetate component were incorporated in different percentages in acrylic bone cements, all loaded with 30 % of hydroxyapatite CORALINA® HAP-200. Curing parameters and mechanical properties were

  3. Avaliação físico-química de bolo de chocolate com coberturas comestíveis à base de gelatina, ácido esteárico, amido modificado ou cera de carnaúba Physical and chemical evaluation of chocolate cake covered with gelatin, stearic acid, modified starch or "carnaúba" wax edible icing

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    Cibele Cristina Osawa

    2009-03-01

    Full Text Available Coberturas comestíveis biodegradáveis são uma alternativa às embalagens sintéticas, que causam preocupações ambientais. Este trabalho avaliou o efeito de diferentes tipos de coberturas sobre propriedades físico-químicas de bolo de chocolate durante a estocagem, em comparação com bolo sem cobertura (CO e bolo sem cobertura embalado em polipropileno (EMB. As seguintes coberturas foram aplicadas sobre os bolos: 10% gelatina (GE, 10% gelatina com 10% ácido esteárico (GE + AE, 18% cera de carnaúba (CE, 10% amido modificado (AM e fondant (FO. Os bolos foram avaliados durante 10 dias de estocagem. FO e EMB apresentaram menor perda de massa, enquanto todos os demais tratamentos apresentaram valores superiores a CO. GE, GE + AE e EMB apresentaram a menor redução da atividade de água, enquanto CE e CO apresentaram a maior redução. As superfícies dos bolos recobertos estavam mais duras que as de CO e EMB. Os maiores valores para dureza e mastigabilidade foram encontrados para CE e CO e os menores, para EMB, GE e GE + AE. Em relação à cor, GE + AE foi diferente dos demais tratamentos, devido à presença do ácido esteárico. Os resultados indicam que a perda de massa dos bolos pode ser atribuída também à perda de água das coberturas.Biodegradable edible icing or frosting is an alternative to synthetic packaging that causes environmental concerns. This work evaluated the effect of different types of frosting on the physical-chemical properties of chocolate cake during storage in comparison to cakes without icing or frosting (C and cakes without frosting packed in polypropylene (CP. The following frostings were applied to the cakes: 10% gelatin (GE, 10% gelatin with 10% stearic acid (GE+SA, 18% "carnaúba" wax (CW, 10% modified starch (MS, and fondant (FO. The cakes were analyzed during 10 days of storage. FO and CP presented the lowest mass loss while all other treatments presented higher values than C. GE, GE+SA, and CP presented

  4. Millî eğitim bakanlığının 652 sayılı kanun hükmünde kararname ile yeniden yapılandırılmasına ilişkin Ankara ili kamu ilkokul ve ortaokul yöneticilerinin görüşleri [Views of the state primary school and secondary school principals in Ankara province about the restructure of Ministry of National Education (By decree law No. 652

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    İnci ÖZTÜRK

    2014-04-01

    Full Text Available Bu araştırmada, Ankara ili merkez ilçelerindeki kamu ilkokul ve ortaokul yöneticilerinin, 652 sayılı Kanun Hükmünde Kararname (KHK ile Millî Eğitim Bakanlığı’nın (MEB yeniden yapılandırılması hakkındaki görüşlerinin belirlenmesi amaçlanmıştır. Tarama modelindeki araştırma, nicel ve nitel yöntemlerin birlikte kullanıldığı “karma araştırma” deseni ile gerçekleştirilmiştir. Araştırmanın nicel boyutuna 276 okul yöneticisi dâhil edilmiş, nitel boyutunda 11 okul yöneticisiyle görüşme yapılmıştır. Araştırmanın nicel boyutunun sonuçlarına göre; 652 sayılı KHK’nın, MEB’in amaç ve politikalarına uygunluğu görüşü en yüksek İktisadî ve İdarî Bilimler Fakültesi- Hukuk Fakültesi mezunlarında gözlenmiş, bunu sırasıyla diğer fakülteler, Eğitim/Eğitim Bilimleri Fakültesi mezunları ve son olarak Fen- Edebiyat Fakültesi mezunlarının izlediği görülmüştür. İlkokul yöneticilerinin, ortaokul yöneticilerine göre 652 sayılı KHK’nın, MEB’in amaç ve politikalarını gerçekleştirme konusunda daha olumlu düşündükleri sonucuna varılmıştır. Araştırmanın nitel boyutu bulgularından; - hizmetlerin değerlendirilmesinde performans değerlendirmesine gidilmesinin, performans ölçütlerinin açık olması ve objektif değerlendirme yapılması koşuluyla uygun olduğu, KHK’nın öngördüğü yapılanma hakkında kamuoyunun yeterince bilgilendirilmediği, eğitim-öğretim hizmetlerinin üst yönetim ve denetim birimlerinde, alan dışından kamu görevlilerinin istihdam edilmemesi gerektiği sonuçlarına ulaşılmıştır.

  5. Original encounter with antigen determines antigen-presenting cell imprinting of the quality of the immune response in mice.

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    Valérie Abadie

    Full Text Available BACKGROUND: Obtaining a certain multi-functionality of cellular immunity for the control of infectious diseases is a burning question in immunology and in vaccine design. Early events, including antigen shuttling to secondary lymphoid organs and recruitment of innate immune cells for adaptive immune response, determine host responsiveness to antigens. However, the sequence of these events and their impact on the quality of the immune response remain to be elucidated. Here, we chose to study Modified Vaccinia virus Ankara (MVA which is now replacing live Smallpox vaccines and is proposed as an attenuated vector for vaccination strategies against infectious diseases. METHODOLOGY/PRINCIPAL FINDINGS: We analyzed in vivo mechanisms triggered following intradermal (i.d. and intramuscular (i.m. Modified Vaccinia virus Ankara (MVA administration. We demonstrated significant differences in the antigen shuttling to lymphoid organs by macrophages (MPhis, myeloid dendritic cells (DCs, and neutrophils (PMNs. MVA i.d. administration resulted in better antigen distribution and more sustained antigen-presenting cells (APCs recruitment into draining lymph nodes than with i.m. administration. These APCs, which comprise both DCs and MPhis, were differentially involved in T cell priming and shaped remarkably the quality of cytokine-producing virus-specific T cells according to the entry route of MVA. CONCLUSIONS/SIGNIFICANCE: This study improves our understanding of the mechanisms of antigen delivery and their consequences on the quality of immune responses and provides new insights for vaccine development.

  6. Investigation of Seatbelt use Frequency of Healthcare Providers in Ankara

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    Serdar Dede

    2013-10-01

    Full Text Available Aim: In Turkey, as in so many other developing countries, traffic accidents appear as a major public health problem that causes damage to thousands of people every year. Use of seat belt which is one of the most important life-saving safety measures in car accidents has not yet reached the desired level. In this study, the sensibilities of hospital health care providers with respect to seat belt use were investigated. Material and Method: After approved to local ethics committee we conducted this study in Hospital. Health care providers were observed at the parking entrance in order to see whether they put on seat belt or not while driving. The personal data of the subjects were obtained either from themselves or through the hospital employee records. Their names, gender, age, marital status, work unit, education level seat belt usage status, and professional parameters were recorded. The Chi-square test or Fisher’s exact test, when appropriate, was used to compareto these proportions in different groups. Results: There was statistically significant difference accordingly to professional groups (p<0.05. In conclusion, we found that health care providers have higher seat belt use rate rather previous study. Discussion: The use of seat belt increases in direct proportion to the education level and socio-economic status.

  7. Psychiatric symptoms of adolescents reared in an orphanage in Ankara.

    Science.gov (United States)

    Kanbur, Nuray; Tüzün, Zeynep; Derman, Orhan

    2011-01-01

    This study compared male adolescents in an orphanage with adolescents raised by their families in terms of psychiatric symptoms, using the Brief Symptom Inventory. Anxiety, depression, negative self, hostility, and Global Severity Index points were significantly higher in adolescents in the orphanage, although they did not reach pathological levels except with respect to hostility. Adolescents reared in orphanages scored high points for hostility, reaching pathological levels. PMID:21980809

  8. Nuclear power: A changing landscape. 7 July 2006, Ankara, Turkey

    International Nuclear Information System (INIS)

    With this brief overview, I have tried to outline some of the challenges and opportunities that lie ahead, with respect to the role of nuclear energy as part of the global energy mix. With its recent decision to embark on a nuclear power programme, Turkey will be facing its own set of technological and institutional challenges. At the IAEA, we stand ready to work with you in finding the solutions that are best suited to your needs and priorities

  9. Performance Appraisal at Four and Five Star Hotels: Ankara Case

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    Yalçın Arslantürk

    2009-12-01

    Full Text Available Nowadays, tourism establishments developing human resources and motivating will make it easier to reach their goals and those who cannot mange to do this will be obsolete in the ever-changing nature of the tourism business. The most important feature of the tourism sector is that it is based on human element and it s labor-intensive. Hence, human element is of great importance. This being the case, the customer satisfaction in accommodation establishments will be greatly dependent on the success of the personnel. Performance appraisal in this regard is a tool used to measure the performance of the employees in establishing job satisfaction and enhancing success. Performance appraisal indicates the performance of the individuals and gives insights as to what should be done to improve the performance. As well as promoting the communication between the employees and the establishment. From the perspectives of the managers, it paves the way for an efficient information flow about the performance of the personnel and makes job planning more rationally. This study first examines performance and performance appraisal. Then, through a field study, deficiencies in the application of performance appraisal were determined in the four and five star hotel establishments. According to the results obtained, some suggestions were put forward.

  10. Teste-padrão de germinação modificado para análise da tolerância de cultivares de soja ao herbicida sulfentrazone Modified germination test for the evaluatiing of tolerance of soybean cultivars to the herbicide sulfentrazone

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    D.L.P. Gazziero

    2005-03-01

    Full Text Available Com o objetivo de avaliar metodologia de laboratório para análise da tolerância de cultivares de soja ao herbicida sulfentrazone, foi conduzido um ensaio na Universidade Estadual de Londrina. Foi utilizado o teste-padrão de germinação com a modificação da solução de embebição, com os cultivares Coodetec 206 e Coodetec 207, considerados tolerante e sensível, respectivamente, ao herbicida sulfentrazone, em campo. Foram preparadas concentrações de 25, 50, 100 e 250 mg L-1 do herbicida sulfentrazone e a solução-padrão com água destilada como testemunha. O papel-toalha foi embebido com solução de sulfentrazone em volume equivalente a três vezes o peso do papel. As unidades experimentais foram rolos de papel, contendo 50 sementes, com quatro repetições, que permaneceram em germinador a 25 ºC por cinco dias, na presença de luz durante o dia. Após esse período foram avaliados o comprimento do hipocótilo, o comprimento da raiz, o comprimento total e o peso das plântulas, em todos os tratamentos. Foi utilizado delineamento experimental inteiramente casualizado, e o procedimento estatístico adotado foi o esquema fatorial 2 (cultivares x 5 (doses, utilizando o teste de Tukey a 5% de probabilidade para comparação das médias. A concentração de 250 mg L-1 de sulfentrazone causou intensa injúria às plântulas, não sendo possível detectar diferenças entre os cultivares. Entretanto, a solução com concentração de 50 mg L-1 de sulfentrazone evidenciou nitidamente a diferença entre os cultivares quanto à tolerância e à sensibilidade, quando utilizado o comprimento do hipocótilo, o comprimento das raízes e o comprimento total de plântulas como características diferenciais, sendo mais evidente a diferença entre os cultivares analisando-se o comprimento do hipocótilo. O teste-padrão de germinação modificado foi adequado para analisar a tolerância dos cultivares de soja ao herbicida sulfentrazone.This experiment

  11. The Immunogenicity of the Tumor-Associated Antigen α-Fetoprotein Is Enhanced by a Fusion with a Transmembrane Domain

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    Lucile Tran

    2012-01-01

    Full Text Available Aim. To investigate the ability of recombinant modified vaccinia virus Ankara (rMVA vector to induce an immune response against a well-tolerated self-antigen. Methods. rMVA vectors expressing different form of α-fetoprotein (AFP were produced and characterized. Naïve mice were vaccinated with MVA vectors expressing the AFP antigen in either a secreted, or a membrane-bound, or an intracellular form. The immune response was monitored by an IFNΓ ELISpot assay and antibody detection. Results. Vaccination with the membrane-associated form of AFP induced a stronger CD8+ T-cell response compared to the ones obtained with the MVA encoding the secreted or the intracellular forms of AFP. Moreover, the vaccination with the membrane-bound AFP elicited the production of AFP-specific antibodies. Conclusions. The AFP transmembrane form is more immunogenic. Expressing a membrane-bound form in the context of an MVA vaccination could enhance the immunogenicity of a self-antigen.

  12. Clinical, manometric and profilometric evaluation after surgery for Hirschsprung's disease: comparison between the modified Duhamel and the transanal rectosigmoidectomy techniques Avaliação clínica, manométrica e profilométrica após correção cirúrgica para doença de Hirschsprung: comparação entre as técnicas de Duhamel modificado e a retossigmoidectomia transanal modificada

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    Elaine Cristina Soares Martins

    2009-10-01

    Full Text Available PURPOSE: To evaluate fecal continence, anorectal manometry (AM and profilometry (P, in patients operated for congenital megacolon, using either the modified Duhamel technique (MDT or the modified transanal rectosigmoidectomy (MTR technique. METHODS: 42 patients were evaluated clinically and via AM and P, for postoperative control. The resting, coughing, voluntary contraction, maintained voluntary contraction and perianal stimulation pressures were investigated. The rectosphincteric reflex was tested and the simple and enhanced pressure curves were evaluated. The three-dimensional profilometric outline was produced. Student's t, chi-squared and Fisher's exact tests were used for statistical analysis (pOBJETIVO: Avaliar a continência fecal, a manometria anorretal (MAR e a profilometria (PFM, em pacientes submetidos a tratamento cirúrgico do megacolo congênito pelas técnicas de Duhamel modificado (DM ou de retossigmoidectomia transanal modificada (RTM. MÉTODOS: 42 pacientes foram clinicamente avaliados e submetidos à MAR e PFM para controle pós-operatório. Foram pesquisadas as pressões no repouso (PR, à tosse (T, à contração voluntária (CV, à contração voluntária mantida (CVM e à estimulação perianal (EPA. O reflexo reto-esficteriano (RRE foi testado e as curvas pressóricas simples e potencializada foram avaliadas. Foi elaborado traçado tridimensional de PFM. Utilizamos os testes t de Student, Qui-Quadrado e exato de Fisher para análise estatística (p<0,05. RESULTADOS: A MAR mostrou médias de PR=53,44 mmHg para DM e 60,67 mmHg para RTM; CV média = 94,50 mmHg para o grupo DM e 95,47 mmHg para o grupo RTM. Não houve diferença estatística entre os grupos. A forma das CPS e CPP não apresentou diferença estatística, independentemente da técnica cirúrgica utilizada CONCLUSÃO: As duas técnicas operatórias foram manometricamente equivalentes. DM determinou maior incidência de constipação pós-operatória que RTM. A

  13. Análise inicial do uso de enxerto tubular orgânico L-D-Hydro - (Eato L-D-Hydro para realização de Blalock-Taussig modificado nas cardiopatias congênitas com hipofluxo pulmonar Initial analysis of the use of the L-D-Hydro (Eato L-D-Hydro organic tubular graft for performing the modified Blalock-Taussig procedure in congenital heart diseases with decreased pulmonary blood flow

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    Wilson Luiz da Silveira

    2005-03-01

    Full Text Available OBJETIVO: Analisar os resultados iniciais da utilização do enxerto tubular orgânico, utilizados para anastomoses sistêmico-pulmonares. MÉTODOS: De março/2002 a abril/2003, 10 pacientes foram submetidos à realização de shunt sistêmico pulmonar tipo Blalock-Taussig modificado utilizando um novo tipo de enxerto biológico originado da artéria mesentérica bovina tratada com poliglicol denominado L-D-Hydro. A idade variou de 3 dias a 7 anos e 60% dos pacientes eram do sexo masculino. O diagnóstico das cardiopatias foi determinado pela ecocardiografia, todos apresentando sinais clínicos de hipóxia severa (cianose. As cardiopatias foram: tetralogia de Fallot (40%, atresia tricúspide (50%, defeito do septo atrioventricular (10%. RESULTADOS: Em 10 pacientes, ocorreu um óbito por sepse e em nove houve melhora imediata na saturação de O2 ao oxímetro de pulso e da pressão parcial de oxigênio à gasometria arterial. Nenhum paciente apresentou obstrução do shunt no pós-operatório imediato ou qualquer outra complicação. Todos os pacientes mostraram shunt pérvio ao exame ecocardiográfico no pós-operatório imediato e tardio, realizado no 3º mês de pós-operatório. Nenhum paciente apresentou sangramento no intra e pós-operatório. CONCLUSÃO: O enxerto tubular L-D-HYDRO demonstrou ser promissor para a realização de shunt sistêmico pulmonar, como alternativa para produtos inorgânicos existentes no mercado, entretanto, temos de ter maior número de implantes e acompanhamento tardio para uma avaliação definitiva.OBJECTIVE: To analyze the initial results of the use of an organic tubular graft for systemic-pulmonary anastomoses. METHODS: From March 2002 to April 2003, 10 patients underwent systemic-pulmonary shunt of the modified Blalock-Taussig type, using a new type of biological graft originating from the bovine mesenteric artery treated with polyglycol, the so-called L-D-Hydro. The patients' ages ranged from 3 days to 7

  14. Caracterización físico-mecánica, térmica y morfológica de polímeros de redes interpenetradas con base en poliuretano obtenido a partir de aceite de ricino y almidón modificados/polimetilmetacrilato (PMMA Physico-mechanical, thermal and morphological Behaviour of simultaneous interpenetrating polymer networks based on polyurethane from modified castor oiland starch/poly(Methyl Methacrylate

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    Manuel F. Valero

    2011-01-01

    Full Text Available El aceite de ricino se modificó por transesterificación con pentaeritritol. Paralelamente, se utilizó la reacción de glucosilación con glicerol para dividir el almidón en unidades monosacáridos. El glucósido obtenido reaccionó por transesterificación con el aceite modificado por transesterificación con pentaeritritol. Se determinó el índice de hidroxilo de los poliol-glucósidos en función del contenido de pentaeritritol y almidón utilizados en la síntesis. Se sintetizaron redes interpenetradas de polímero de manera simultánea (SINs con diferentes relaciones en peso poliuretano/PMMA de 100/0, 90/10, 80/20, 70/30, 60/40 y 50/50. Para la síntesis de la red de poliuretano (PU se utilizaron los poliol-glucósidos y diisocianato de isoforona (IPDI o con una relación NCO/OH = 1 y para la síntesis de la red de polimetilmetacrilato (PMMA se utilizó peróxido de benzoílo como agente iniciador y dimetacrilato de etilenglicol como agente entrecruzante. Los SINs se caracterizaron por sus propiedades fisicomecánicas como resistencia al ataque químico, dureza, resistencia a la tensión y elongación de ruptura. La estabilidad térmica de los SINs se determinó usando análisis termogravimétrico (TGA. La morfología de superficie se determinó usando microscopia electrónica de barrido (SEM y mostró una morfología de dos fases para todos los SINs.Castor oil was modified by transesterification with pentaerithritol. In a parallel step, starch was divided into monosaccharide units by glycosylation in order to obtain products with high hydroxyl content. The values of hydroxyl index were measured according to the content of pentaerithritol and starch used in the synthesis. Novel simultaneous interpenetrating polymer networks (SINs of polyurethane (PU and poly(methyl methacrylate (PMMA in different weight ratios of 100/0, 90/10, 80/20, 70/30, 60/40 and 50/50 were prepared based on polyol-glucosides. The polyurethane network was created by

  15. Controle de vetores utilizando mosquitos geneticamente modificados Control de vectores utilizando mosquitos genéticamente modificados Control of vector populations using genetically modified mosquitoes

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    André Barreto Bruno Wilke

    2009-10-01

    Full Text Available Formas químicas de controle de mosquitos vetores são ineficazes, levando ao desenvolvimento de novas estratégias. Assim, foi realizada revisão das estratégias de controle genético de populações de mosquitos vetores baseada na técnica do inseto estéril. Uma delas consiste na liberação de machos esterilizados por radiação, a outra, na integração de um gene letal dominante associado a um promotor específico de fêmeas imaturas. Entre as vantagens sobre outras técnicas biológicas e químicas de controle de vetores estão: alta especificidade, não prejudicial ao meio ambiente, baixo custo de produção e alta eficácia. O uso desta técnica de modificação genética pode vir a ser uma importante ferramenta do manejo integrado de vetores.Formas químicas de control de mosquitos vectores son ineficaces, llevando al desarrollo de nuevas estrategias. Así, fue realizada revisión de las estrategias de control genético de poblaciones de mosquitos vectores basada en la técnica del insecto estéril. Una de ellas consiste en la liberación de machos esterilizados por radiación, la otra, en la integración de un gen letal dominante asociado a un promotor específico de hembras inmaduras. Entre las ventajas sobre otras técnicas biológicas y químicas de control de vectores están: la alta especificidad, no prejudicial al ambiente, bajo costo de producción y alta eficiencia. El uso de esta técnica de modificación genética puede ser una importante herramienta del manejo integrado de vectores.The ineffectiveness of current strategies for chemical control of mosquito vectors raises the need for developing novel approaches. Thus, we carried out a literature review of strategies for genetic control of mosquito populations based on the sterile insect technique. One of these strategies consists of releasing radiation-sterilized males into the population; another, of integrating a dominant lethal gene under the control of a specific promoter into immature females. Advantages of these approaches over other biological and chemical control strategies include: highly species-specific, environmentally safety, low production cost, and high efficacy. The use of this genetic modification technique will constitute an important tool for integrated vector management.

  16. Vergleich von rekombinanten Vaccinia- und DNA-Vektoren zur Tumorimmuntherapie im C57BL/6-Mausmodell

    Science.gov (United States)

    Johnen, Heiko

    2002-10-01

    In der vorliegenden Arbeit wurden Tumorimpfstoffe auf der Basis des Plasmid-Vektors pCI, modified vaccinia virus Ankara (MVA) und MVA-infizierten dendritischen Zellen entwickelt und durch Sequenzierung, Western blotting und durchflußzytometrische Analyse überprüft. Die in vivo Wirksamkeit der Vakzinen wurde in verschiedenen Tumormodellen in C57BL/6 Mäusen verglichen. Die auf dem eukaryotischen Expressionsvektor pCI basierende DNA-Vakzinierung induzierte einen sehr wirksamen, antigenspezifischen und langfristigen Schutz vor Muzin, CEA oder beta-Galactosidase exprimierenden Tumoren. Eine MVA-Vakzinierung bietet in den in dieser Arbeit durchgeführten Tumormodellen keinen signifikanten Schutz vor Muzin oder beta-Galactosidase exprimierenden Tumoren. Sowohl humane, als auch murine in vitro generierte dendritische Zellen lassen sich mit MVA – im Vergleich zu anderen viralen Vektoren – sehr gut infizieren. Die Expressionsrate der eingefügten Gene ist aber gering im Vergleich zur Expression in permissiven Wirtszellen des Virus (embryonale Hühnerfibroblasten). Es konnte gezeigt werden, daß eine MVA-Infektion dendritischer Zellen ähnliche Auswirkungen auf den Reifezustand humaner und muriner dendritischer Zellen hat, wie eine Infektion mit replikationskompetenten Vakzinia-Stämmen, und außerdem die Hochregulation von CD40 während der terminalen Reifung von murinen dendritischen Zellen inhibiert wird. Die während der langfristigen in vitro Kultur auf CEF-Zellen entstandenen Deletionen im MVA Genom führten zu einer starken Attenuierung und dem Verlust einiger Gene, die immunmodulatorische Proteine kodieren, jedoch nicht zu einer Verminderung des zytopathischen Effekts in dendritischen Zellen. Die geringe Expressionsrate und die beobachtete Inhibition der Expression kostimulatorischer Moleküle auf dendritischen Zellen kann für eine wenig effektive Induktion einer Immunantwort in MVA vakzinierten Tieren durch cross priming oder die direkte Infektion antigenpr

  17. Grado de conocimiento y actitudes de los consumidores espanoles hacia los alimentos modificados geneticamente

    OpenAIRE

    Noomene, Rouhia; Gil, Jose Maria

    2006-01-01

    Los alimentos transgenicos han sido objecto de un consuderabke debate en los ultimos anos, manteniendose posturas radicalmente opuestas. A pesar de que la aceptacion entre los productores es notable, los consumidores, sobre todo en Europa, se han mostrado tradicionalmente reacios a este tipo de productos. Una vez que se ha aprobado la regulacion sobre el etiquetado de estos alimentos y, por tanto, que el consumidor va a disponer de cierta informacion sobre la naturaleza de los productos que v...

  18. Cultivos de queratinocitos derivados de piel humana modificados genéticamente

    OpenAIRE

    Luz Restrepo; Clara Chamorro; Martha Arango

    2001-01-01

    Existen diversos métodos para modificar los queratinocitos (1).
    Uno de ellos, los vectores retrovirales, ha mostrado mayor eficiencia
    en la modificación genética de estas células, las cuales pueden ser
    establemente transducidas con estos vectores y ser un blanco atractivo para la terapia génica en la piel (2).
    El objetivo fue evaluar, en cultivos primarios de queratinocitos, la eficiencia de la transducción mediada por el vector retroviral ...

  19. Alimentos geneticamente modificados: a engenharia genética no nosso prato

    OpenAIRE

    Gaspar, Pedro João Soares

    2009-01-01

    A Alimentação é um tema que envolve e interessa a todos os seres vivos. No caso dos humanos, e dada a sua racionalidade, este assunto reveste-se de particular importância, uma vez que lhe é reconhecido, de forma inequívoca, um papel determinante na sua sobrevivência, desenvolvimento, saúde e bem-estar. Na década de 60, milhões de pessoas, nomeadamente na China, Índia e Paquistão, estavam em risco de morrer de desnutrição, e foram salvos por uma combinação de herbicidas, adubos e s...

  20. Granada modificado con restricción geométrica

    Directory of Open Access Journals (Sweden)

    Susana González Santana

    2013-03-01

    Full Text Available El desarrollo de la química computacional la ha convertido en una sólida e importante disciplina de la química moderna. Numerosos métodos han sido implementados con objetivos muy diversos. La metodología de Hipersuperficie de Múltiples Mínimos (MMH permite el estudio de la influencia de disolventes sobre un soluto, y en general de interacciones débiles, haciendo uso de las herramientas de la química cuántica y la termodinámica estadística. Con el objetivo de explorar el espacio de configuraciones se crea un ensemble N, V, T empleando el programa GRANADA para generar  celdas o supermoléculas de configuraciones diferentes del (o los disolvente(s que rodean al soluto. El programa GRANADA no permite restringir la generación de configuraciones del disolvente a una zona del espacio específica respecto al soluto. Esta restricción es útil para evitar la exploración innecesaria (y el consiguiente costo computacional de las zonas del espacio que no son de interés o no representan la realidad física del problema. Por este motivo, en este trabajo se modificó el código fuente del GRANADA para implementar la restricción espacial en la generación de las celdas. Además presentamos un ejemplo de las ventajas de aplicar la restricción para la modelación de la influencia de grupos superficiales en la adsorción de Hexaclorociclohexano en Carbón Activado utilizando un modelo simple.

  1. Predictor de Smith modificado mediante un modelo interno, robusto a perturbaciones externas no medibles.

    OpenAIRE

    Fernando Castillo García; Vicente Feliu Batlle; Raúl Rivas Pérez; Ivón O Benítez González

    2010-01-01

    Se propone una modificación de la estructura del predictor de Smith mediante un modelo interno que posibilita aumentar su rechazo al efecto de las per-turbaciones externas no medibles en comparación con la estructura clásica del predictor de Smith. Los resultados obtenidos se aplican en el diseño de un sistema de control del proceso de variación de la temperatura del jugo en los calentadores de una fábrica de azúcar. Los resultados de la simulación del sistema diseñado mostraron su efecti...

  2. Queratinocitos humanos modificados genéticamente por medio de un vector retroviral

    OpenAIRE

    Arango M.; Restrepo L.; Chamorro C.

    2001-01-01

    Los queratinocitos poseen características ideales para la terapia génica: accesibles, modifi-cables por vectores retrovirales, conservan in vitro sus propiedades de proliferación y diferen-ciación, fácil remoción por efectos adversos. Nuestro objetivo fue evaluar estas células comoblanco de transferencia de genes empleando el vector retroviral Foch-29 NeoR.

  3. Propiedades de los aceites vegetales modificados por hidrogenacion, interesterificación y fraccionamiento

    OpenAIRE

    Grompone, M. A.

    1992-01-01

    The advent of techniques of chemical processing (hydrogenation and interesterification) and physical processing (fractionation) provides the manufacturer the capacity of modifying one or many physicochemical properties of an oil or fat. In the present paper the influence of the chemical interesterification and thermal fractionation without solvent on the physical properties (melting point, solid fat index) of the products obtained by partial hydrogenation of soybean oil is studied. Prop...

  4. Propiedades de los aceites vegetales modificados por hidrogenacion, interesterificación y fraccionamiento

    Directory of Open Access Journals (Sweden)

    Grompone, M. A.

    1992-12-01

    Full Text Available The advent of techniques of chemical processing (hydrogenation and interesterification and physical processing (fractionation provides the manufacturer the capacity of modifying one or many physicochemical properties of an oil or fat. In the present paper the influence of the chemical interesterification and thermal fractionation without solvent on the physical properties (melting point, solid fat index of the products obtained by partial hydrogenation of soybean oil is studied. Properties of mixtures of partially hydrogenated soybean oil and totally hydrogenated coconut oil are also determined. The examples studied show that knowing the changes produced by current modification methods is useful to design fatty products for specific purposes.

    El advenimiento de técnicas de procesamiento químico (hidrogenación e interesterificación y físico (fraccionamiento proveen al industrial de la capacidad de modificar una o varias de las propiedades fisicoquímicas de una grasa o aceite. En este trabajo se estudia de un modo sistemático la influencia de la interesterificación química y del fraccionamiento térmico sin solventes sobre las propiedades físicas (punto de fusión, índice de grasa sólida de los productos obtenidos por hidrogenación parcial de aceite de soya. También se determinan las propiedades de mezclas de aceite de soya parcialmente hidrogenado con aceite de coco totalmente hidrogenado. Los ejemplos estudiados muestran que el conocimiento de los cambios ocasionados por los procesos de modificación corrientes son útiles para diseñar productos grasos para fines específicos.

  5. Cementos oseos acrílicos modificados con hidroxiapatita para implantes óseos

    Directory of Open Access Journals (Sweden)

    Mónica López Hernández

    2006-01-01

    Full Text Available El desarrollo dinámico, tan característico de nuestras sociedades modernas, ha provocado un aumento en la expectativa de vida del hombre. El envejecimiento progresivo de la población se ha visto acompañado por un notable incremento de las afecciones articulares degenerativas que requieren de sustitución protésica. En las últimas décadas las investigaciones en el campo de los biomateriales han estado dirigidas a la búsqueda de materiales con las características adecuadas para la restauración o sustitución del tejido óseo. Entre ellos se encuentran, los cementos óseos, los cuales se han convertido en los últimos años en biomateriales de gran utilidad como sustitutos óseos. El estudio de diferentes formulaciones químicas, de las propiedades físicas y mecánicas, el efecto de posibles aditivos, la biocompatibilidad y las propiedades biológicas de estos cementos tienen como objetivo principal la obtenci ón de biomateriales con variadas aplicaciones en los ámbitos quirúrgicos en que se requiere la sustitución y regeneración ósea. Se revisaron los aspectos más importantes de los cementos óseos acrílicos en cuanto a sus propiedades físicas, químicas, mecánicas, así como los métodos más usados para la preparación del polímero y las formulaciones de los cementos. Se discuten también las experiencias clínicas acumuladas con diferentes tipos de cementos acrílicos, sus ventajas y limitaciones tanto en la fijación de endoprótesis articulares como en el relleno de defectos óseos y los intentos por mejorar su biocompatibilidad mediante cargas de hidroxiapatita (HA. La obtención de materiales compuestos por polimetacrilato de metilo-HA parece ser una vía factible que promete resultados prominentes para la fabricación de nuevos cementos quirúrgicos con mejores propiedades para ser usados en la cirugía reconstructiva del hueso en general.

  6. Agregado reciclado empregado na produção de concreto modificado com aditivo

    OpenAIRE

    Márcio Joaquim Estefano de Oliveira

    2006-01-01

    O emprego de agregado reciclado de resíduo de concreto e de rejeitos cerâmicos se apresenta como alternativa promissora para a fabricação de componentes e elementos de construção civil, com a finalidade de atender às questões técnicas, econômicas, sociais e ambientais. A matéria-prima empregada na pesquisa foi o resíduo de concreto descartado pelas obras de construção civil que, muitas vezes, contém restos de materiais cerâmicos provenientes de blocos e azulejos, com idad...

  7. Eletrodos modificados com DNA: uma nova alternativa em eletroanálise

    Directory of Open Access Journals (Sweden)

    La-Scalea Mauro A.

    1999-01-01

    Full Text Available The first studies about DNA electrochemistry appeared at the end of the fifties. The voltammetric techniques became important tool for the DNA conformational analysis, producing evidences about DNA double helix polimorphism. The new techniques based on electrodes modification with nucleic acid enlarged the use of the electrochemical methods on the DNA research. DNA electrochemical biosensors are able to detect specific sequences of DNA bases, becoming important alternative for the diagnosis of disease, as well as in the carcinogenic species determination. Besides, the use of DNA biosensors in the mechanism study of biological drug actions can be useful for drug design.

  8. Influencia del proceso de esterilización en la mojabilidad del titanio modificado superficialmente

    OpenAIRE

    Pergueroles, M.; Menéndez Crespo, Emilio; Aparicio, C; Planell Estany, Josep Anton; Gil, F. X. (Francesc Xavier)

    2004-01-01

    El objetivo de este trabajo es estudiar el cambio en las propiedades energéticas de muestras de titanio comercialmente puro debido a distintos tratamientos superficiales y a distintos métodos de esterilización; con la finalidad de entender cómo afectan estos cambios a los procesos biológicos de los implantes de titanio. Los distintos tratamientos superficiales utilizados fueron: la formación de rugosidad mediante la proyección de partículas abrasivas, el granallado, y la esteri...

  9. Fluxo gênico em milho geneticamente modificado com resistência a insetos

    OpenAIRE

    Vivian Elias Nascimento; Édila Vilela de Resende Von Pinho; Renzo Garcia Von Pinho; João Cândido de Souza; André Domingos do Nascimento Júnior

    2012-01-01

    O objetivo deste trabalho foi estimar o fluxo gênico em milho transgênico com resistência a insetos, em lavouras comerciais. Amostras de grãos foram coletadas em lavouras com milho convencional e transgênico, nos municípios de: Itumirim, Uberlândia, Paracatu e Tupaciguara, MG; Itapetininga e Pedrinhas, SP; e Assaí e Ponta Grossa, PR. As amostras foram coletadas em lavouras de milho convencional, a partir de 5 m de distância da fonte com o milho transgênico. Foram coletadas dez espigas de plan...

  10. Cementos oseos acrílicos modificados con hidroxiapatita para implantes óseos

    OpenAIRE

    Mónica López Hernández; Ramón González Santos

    2006-01-01

    El desarrollo dinámico, tan característico de nuestras sociedades modernas, ha provocado un aumento en la expectativa de vida del hombre. El envejecimiento progresivo de la población se ha visto acompañado por un notable incremento de las afecciones articulares degenerativas que requieren de sustitución protésica. En las últimas décadas las investigaciones en el campo de los biomateriales han estado dirigidas a la búsqueda de materiales con las características adecuadas para la restauración o...

  11. Animales genéticamente modificados, primates no humanos. (La visión europea

    Directory of Open Access Journals (Sweden)

    Velayos Castelo, Carmen

    2008-04-01

    Full Text Available European citizens think that more needs to be done to improve the level of welfare/protection of animals used in experiments. In recent years, it has become increasingly apparent that the existing legislation for the protection of animals used in experiments (Directive 86/609/EEC. needs to be revised in order to promote improvements in the welfare of laboratory animals and to further foster the development of alternative methods. The UE citizens’ consultation received the third largest number of responses to a Commission internet consultation ever. Since 1986 important progress has been made in science and new techniques have become available, such as use of transgenic animals, xenotransplantation and cloning. These require specific attention, which the current Directive does not provide for. Nor is the use of animals with a higher degree of neurophysiological sensitivity specifically regulated, such as in the case of non-human primates. This essay wants to reflect about experiments with primates and transgenic animals from the perspective of the institutional european ethics.Los ciudadanos europeos creen que se necesita hacer algo más para mejorar el nivel de bienestar/protección de los animales que son utilizados en experimentos. En los últimos años, se ha hecho cada vez más evidente que la legislación actual para la protección de los animales utilizados en experimentación (Directiva 86/609(EEC necesita ser revisada para promover mejoras en el bienestar de los animales de laboratorio, así como para facilitar el desarrollo de métodos alternativos. La consulta de ciudadanos europeos ha recibido el tercer número más alto de respuestas que ha tenido jamás una consulta de la Comisión por internet. Desde 1986, ha habido importantes progresos en ciencia y hay nuevas técnicas disponibles, como la utilización de animales transgénicos, los xenotrasplantes y la clonación. Dichas técnicas requieren una atención específica que la Directiva actual no procura. Como tampoco está regulada específicamente la investigación con animales con el más alto grado de sensibilidad neurofisiológica, como los primates no humanos. Este trabajo quiere reflexionar sobre experimentos con primates y animales transgénicos desde la perspectiva de la ética institucional europea.

  12. Modified silicates applied in adsorption of heavy metal; Silicatos modificados aplicados na adsorcao de metal pesado

    Energy Technology Data Exchange (ETDEWEB)

    Farias, M.C.M. de [Universidade Federal de Campina Grande (UFCG), PB (Brazil). Unidade Academica de Engenharia de Materiais; Raposo, C.M.O., E-mail: raposo@dmg.ufcg.edu.b [Universidade Federal de Campina Grande (UFCG), Campina Grande, PB (Brazil). Unidade Academica de Mineracao e Geologia

    2010-07-01

    The levels of heavy metals in the environment has increased considerably in recent decades due to various human activities, which cause serious pollution problems, both in aquatic systems and in soil. The clay minerals present himself as amenable to the adsorption of metal ions and, sometimes, taking the advantage of being abundant and inexpensive. Vermiculite has intrinsic characteristics which favor its use as adsorbent. In this work, we investigate the adsorption of lead (II) from aqueous solutions by vermiculite fractions in commercial, fine to medium in molar concentration between 1-4 mmol (s). The samples provided by the Uniao Brasileira de Mineracao/Paraiba/Brazil were modified thermal and organically. The results of X-ray diffraction associated with the results of X-ray fluorescence showed that the average fraction vermiculite exfoliated organically modified responded most significantly to the adsorption process when compared to vermiculite fine fraction under the same conditions. (author)

  13. Estudios de nuevos films de PMMA modificados con líquido iónico

    OpenAIRE

    López Moreno, Carlos

    2015-01-01

    La obtención de films poliméricos con nuevas dispersiones de líquidos iónicos constituye una modalidad de estudio innovadora. En este trabajo en particular, se pretende dar comienzo a una nueva etapa investigadora obteniendo los primeros resultados ante los diferentes ensayos que se han realizado con este material. Se ha obtenido y caracterizado film de polimetilmetacrilato a partir del líquido iónico tetrafluoroborato de 1-octil,3-metilimidazolio. Se han estudiado las propiedades térmicas...

  14. Modelado de fractura dúctil sobre aceros modificados superficialmente 545-A

    OpenAIRE

    Rodríguez D´derlée, Sahel

    2015-01-01

    Históricamente la fractura ha sido considerada siempre como un efecto indeseado entre los materiales, dado que su aparición supone un cese del material en servicio, puesto que un material fracturado carece de importancia desde el punto de vista comercial. Consecuentemente, la Mecánica de Fractura ha experimentado un desarrollo importante en las últimas décadas como no lo hizo en toda la historia de los materiales. El desarrollo de nuevos campos a nivel científico y técnico han estado de la ma...

  15. Comportamiento de una Mezcla Densa en Caliente Elaborada con Asfaltos Modificados con Asfaltita

    Directory of Open Access Journals (Sweden)

    Hugo A. Rondón-Quintana

    2009-06-01

    Full Text Available Laboratory tests were used to evaluate the effect on the mechanical properties of a hot asphalt mix (MDC-2 as per INVIAS, 2007 specifications due to the addition by wet way of a natural asphaltite from the San Alberto Mine (Santander, Colombia. The strength under monotonic load, resilient modulus and rutting were evaluated. Two asphalt cements (CA were used, CA 80-100 from the Barrancabermeja refinery (Colombia and CA 60-70 from Apiay (Colombia. The results show that the mechanical properties evaluated were higher for the MDC2 mixes modified with asphaltite compared with mixtures with asphalts without additives. Additionally penetration tests at different temperatures and softening points were conducted on asphalt cementswith and without additive. The asphaltite produces higher penetration resistance and lower thermal flow susceptibility.

  16. Imunoprofilaxia anti-herpética utilizando vírus geneticamente modificado: vacina DISC

    Directory of Open Access Journals (Sweden)

    Lupi Omar

    2003-01-01

    Full Text Available As vacinas anti-herpéticas podem atuar de forma profilática ou terapêutica contra a infecção pelo herpes simples. Diversos tipos de vacinas foram avaliados no passado com resultados pouco efetivos, tais como aquelas que utilizaram vírus vivos, porém atenuados, e as que utilizaram subunidades glicoprotéicas. As novas vacinas do tipo DISC, com partículas infectivas incapacitadas para mais de um ciclo replicativo, são desenhadas para combinar a segurança e as vantagens das vacinas que utilizam vírus atenuados com a imunogenicidade das que usam vírus vivos. Nas vacinas DISC utiliza-se um vírus cujo gene para a glicoproteína H foi removido. Torna-se, assim, capaz de infectar células humanas, exatamente como o vírus natural, mas sua progênie não pode mais completar o ciclo replicativo. São partículas virais não patogênicas, capazes de induzir ampla resposta de linfócitos T citotóxicos e da imunidade humoral contra antígenos herpéticos.

  17. Operation experiences with a 30 kV/100 MVA high temperature superconducting cable system

    DEFF Research Database (Denmark)

    Tønnesen, Ole; Däumling, Manfred; Jensen, Kim Høj; Kvorning, Svend; Olsen, Søren K; Træholt, Chresten; Veje, Erling; Willen, Dag; Østergaard, Jacob

    2004-01-01

    A superconducting cable based on Bi-2223 tape technology has been developed, installed and operated in the public network of Copenhagen Energy in a two-year period between May 2001 and May 2003. This paper gives a brief overview of the system and analyses some of the operation experiences. The aim...... operating current of 1157 A. The operation experiences include over-currents of 6 kA due to faults on peripheral lines, commissioning, servicing and failure responses on the cooling system, continuous 24 h, 7 day per week monitoring and performance of the alarm system. The implications of these experiences...... of this demonstration project is to gain experience with HTS cables under realistic conditions in a live distribution network. Approximately 50 000 utility customers have their electric power supplied through the HTS cable. The cable system has delivered 226 GW h of energy and reached a maximum...

  18. Inoculación de granadilla Passiflora ligularis L. con MVA

    OpenAIRE

    Sánchez de Prager Marina; Rodríguez G. Angela María; Hurtado Miguel

    1995-01-01

    For evaluating the possibilities of substitution of the phosphoric fertilization by means of the inoculation with micorrizogen fungi, in a granadilla crop in nursery stage; it was used hillside type soil with a pH of 4.8 and 2.0 ppm of P, in natural conditions and previous dessinfection. Individually was inoculated Acaulospora foveata, Glomus occultum, Acaulospora longula and it had a control in the native flora. Two hundred spores per experimental unit were applied in small granadil...

  19. Inoculación de granadilla Passiflora ligularis L. con MVA

    Directory of Open Access Journals (Sweden)

    Sánchez de Prager Marina

    1995-06-01

    Full Text Available

    For evaluating the possibilities of substitution of the phosphoric fertilization by means of the inoculation with micorrizogen fungi, in a granadilla crop in nursery stage; it was used hillside type soil with a pH of 4.8 and 2.0 ppm of P, in natural conditions and previous dessinfection. Individually was inoculated Acaulospora foveata, Glomus occultum, Acaulospora longula and it had a control in the native flora. Two hundred spores per experimental unit were applied in small granadilla plants transplanted recently. Three levels of P fertilization were proved (0, 25 and 50 kg of Triple Superphosphate per ha. After four months the variable were evaluated. Disinfection had a negative influence in plant development and in the efficientcy of Superphosphate captation. The best response in the development variables evaluated was obtained using 50 kg of Triple Superphosphate per ha, which indicates that in such type of soil, with low P availability, VAM do not reduce the use of fertilizer, but increases its efficiency in utilization for the plant. A. longula was the more effective micorrizal variety.

    Para evaluar las posibilidades de sustitución de fertilización fosfórica, mediante inoculación con hongos micorrizógenos, en el cultivo de la granadilla Passiflora ligularis L., en etapa de vivero, se trabajó con un suelo característico de zona de ladera con pH 4.8 y 2.1 ppm de P, en condiciones naturales y previa des infestación. Se inoculó individualmente: Acaulospora foveata, Glomus occultum, Acaulospora longula y se tuvo una condición testigo correspondiente a flora nativa. Se aplicó un promedio de 200 esporas/unidad experimental, en las plántulas de granadilla recién trasplantadas. Se probaron tres niveles de fertilización: (0, 25 y 50 kg de superfosfato triple/ha. La evaluación de las variables se hizo a los 4 meses de trasplante. La desinfestación influye negativamente en el desarrollo de las plantas y en la eficiencia de captación del superfosfato triple. La mejor respuesta en las variables de desarrollo evaluadas, se obtuvo con la aplicación de 50 kg de superfosfato triple/ha, lo cual indica que en este suelo, dada la baja disponibilidad de P, el recurso micorriza no permite disminuir dosis de fertilizante, sino que incrementa considerablemente su eficiencia de utilización por la planta.  A. longula se comportó como la cepa más efectiva.

  20. Inoculación de granadilla passiflora ligularis l. con mva

    OpenAIRE

    Rodríguez G., Angela María; Hurtado, Miguel; Sánchez de Prager, Marina

    2010-01-01

    Para evaluar las posibilidades de sustitución de fertilización fosfórica, mediante inoculación con hongos micorrizógenos, en el cultivo de la granadilla Passiflora ligularis L., en etapa de vivero, se trabajó con un suelo característico de zona de ladera con pH 4.8 y 2.1 ppm de P, en condiciones naturales y previa des infestación. Se inoculó individualmente: Acaulospora foveata, Glomus occultum, Acaulospora longula y se tuvo una condición testigo correspondiente a flora nativa. Se aplicó un p...

  1. In-Situ MVA of CO2 Sequestration Using Smart Field Technology

    Energy Technology Data Exchange (ETDEWEB)

    Mohaghegh, Shahab D. [West Virginia Univ. Research Corporation, Morgantown, WV (United States)

    2014-09-01

    Capability of underground carbon dioxide storage to confine and sustain injected CO2 for a long period of time is the main concern for geologic CO2 sequestration. If a leakage from a geological CO2 sequestration site occurs, it is crucial to find the approximate amount and the location of the leak, in a timely manner, in order to implement proper remediation activities. An overwhelming majority of research and development for storage site monitoring has been concentrated on atmospheric, surface or near surface monitoring of the sequestered CO2 . This study aims to monitor the integrity of CO2 storage at the reservoir level. This work proposes developing in-situ CO2 Monitoring and Verification technology based on the implementation of Permanent Down-hole Gauges (PDG) or “Smart Wells” along with Artificial Intelligence and Data Mining (AI&DM). The technology attempts to identify the characteristics of the CO2 leakage by de-convolving the pressure signals collected from Permanent Down-hole Gauges (PDG). Citronelle field, a saline aquifer reservoir, located in the U.S. was considered as the basis for this study. A reservoir simulation model for CO2 sequestration in the Citronelle field was developed and history matched. PDGs were installed, and therefore were considered in the numerical model, at the injection well and an observation well. Upon completion of the history matching process, high frequency pressure data from PDGs were generated using the history matched numerical model using different CO2 leakage scenarios. Since pressure signal behaviors were too complicated to de-convolute using any existing mathematical formulations, a Machine Learning-based technology was introduced for this purpose. An Intelligent Leakage Detection System (ILDS) was developed as the result of this effort using the machine learning and pattern recognition technologies. The ILDS is able to detect leakage characteristics in a short period of time (less than a day from its occurrence) demonstrating the capability of the system in quantifying leakage characteristics subject to complex rate behaviors. The performance of ILDS is examined under different conditions such as multiple well leakages, cap rock leakage, availability of an additional monitoring well, presence of pressure drift and noise in the pressure sensor and uncertainty in the reservoir model.

  2. Operation experiences with a 30 kV/100 MVA high temperature superconducting cable system

    International Nuclear Information System (INIS)

    A superconducting cable based on Bi-2223 tape technology has been developed, installed and operated in the public network of Copenhagen Energy in a two-year period between May 2001 and May 2003. This paper gives a brief overview of the system and analyses some of the operation experiences. The aim of this demonstration project is to gain experience with HTS cables under realistic conditions in a live distribution network. Approximately 50 000 utility customers have their electric power supplied through the HTS cable. The cable system has delivered 226 GW h of energy and reached a maximum operating current of 1157 A. The operation experiences include over-currents of 6 kA due to faults on peripheral lines, commissioning, servicing and failure responses on the cooling system, continuous 24 h, 7 day per week monitoring and performance of the alarm system. The implications of these experiences for the future applications of HTS cable systems are analysed

  3. 1H NMR Spectroscopy and MVA Analysis of Diplodus sargus Eating the Exotic Pest Caulerpa cylindracea

    Directory of Open Access Journals (Sweden)

    Sandra A. De Pascali

    2015-06-01

    Full Text Available The green alga Caulerpa cylindracea is a non-autochthonous and invasive species that is severely affecting the native communities in the Mediterranean Sea. Recent researches show that the native edible fish Diplodus sargus actively feeds on this alga and cellular and physiological alterations have been related to the novel alimentary habits. The complex effects of such a trophic exposure to the invasive pest are still poorly understood. Here we report on the metabolic profiles of plasma from D. sargus individuals exposed to C. cylindracea along the southern Italian coast, using 1H NMR spectroscopy and multivariate analysis (Principal Component Analysis, PCA, Orthogonal Partial Least Square, PLS, and Orthogonal Partial Least Square Discriminant Analysis, OPLS-DA. Fish were sampled in two seasonal periods from three different locations, each characterized by a different degree of algal abundance. The levels of the algal bisindole alkaloid caulerpin, which is accumulated in the fish tissues, was used as an indicator of the trophic exposure to the seaweed and related to the plasma metabolic profiles. The profiles appeared clearly influenced by the sampling period beside the content of caulerpin, while the analyses also supported a moderate alteration of lipid and choline metabolism related to the Caulerpa-based diet.

  4. Liquid helium rotational reservoir management in a 20 MVA superconducting generator

    International Nuclear Information System (INIS)

    This chapter discusses the liquid helium (LHe) pool management in a rotating superconducting magnet system. A superconducting rotor was tested for more than 250 hours at 3600 rpm with a reservoir (pool) of liquid helium. The rotor is uniquely designed LHe dewar with encapsulated NbTi superconducting racetrack windings encased in an aluminium support system. Topics considered include the LHe flow circuit and control, cooldown experience, LHe pool level control, transfer line losses, and the identification of improvements required. A superconducting rotor and a specially designed air-gap winding stator were exercised for the first time over their full range of capability during a two-month period. It is concluded that the test experiences and test data will greatly contribute to the knowledge of designing and operating superconducting rotors and generators

  5. Biosynthesis of β-carotene in engineered E. coli using the MEP and MVA pathways

    OpenAIRE

    Yang, Jianming; Guo, Lizhong

    2014-01-01

    Background β-carotene is a carotenoid compound that has been widely used not only in the industrial production of pharmaceuticals but also as nutraceuticals, animal feed additives, functional cosmetics, and food colorants. Currently, more than 90% of commercial β-carotene is produced by chemical synthesis. Due to the growing public concern over food safety, the use of chemically synthesized β-carotene as food additives or functional cosmetic agents has been severely controlled in recent years...

  6. A Chimeric HIV-1 gp120 Fused with Vaccinia Virus 14K (A27 Protein as an HIV Immunogen.

    Directory of Open Access Journals (Sweden)

    Aneesh Vijayan

    Full Text Available In the HIV vaccine field, there is a need to produce highly immunogenic forms of the Env protein with the capacity to trigger broad B and T-cell responses. Here, we report the generation and characterization of a chimeric HIV-1 gp120 protein (termed gp120-14K by fusing gp120 from clade B with the vaccinia virus (VACV 14K oligomeric protein (derived from A27L gene. Stable CHO cell lines expressing HIV-1 gp120-14K protein were generated and the protein purified was characterized by size exclusion chromatography, electron microscopy and binding to anti-Env antibodies. These approaches indicate that gp120-14K protein is oligomeric and reacts with a wide spectrum of HIV-1 neutralizing antibodies. Furthermore, in human monocyte-derived dendritic cells (moDCs, gp120-14K protein upregulates the levels of several proinflammatory cytokines and chemokines associated with Th1 innate immune responses (IL-1β, IFN-γ, IL-6, IL-8, IL-12, RANTES. Moreover, we showed in a murine model, that a heterologous prime/boost immunization protocol consisting of a DNA prime with a plasmid expressing gp120-14K protein followed by a boost with MVA-B [a recombinant modified vaccinia virus Ankara (MVA expressing HIV-1 gp120, Gag, Pol and Nef antigens from clade B], generates stronger, more polyfunctional, and greater effector memory HIV-1-specific CD4+ and CD8+ T-cell immune responses, than immunization with DNA-gp120/MVA-B. The DNA/MVA protocol was superior to immunization with the combination of protein/MVA and the latter was superior to a prime/boost of MVA/MVA or protein/protein. In addition, these immunization protocols enhanced antibody responses against gp120 of the class IgG2a and IgG3, together favoring a Th1 humoral immune response. These results demonstrate that fusing HIV-1 gp120 with VACV 14K forms an oligomeric protein which is highly antigenic as it activates a Th1 innate immune response in human moDCs, and in vaccinated mice triggers polyfunctional HIV-1

  7. A Chimeric HIV-1 gp120 Fused with Vaccinia Virus 14K (A27) Protein as an HIV Immunogen

    Science.gov (United States)

    Vijayan, Aneesh; García-Arriaza, Juan; C. Raman, Suresh; Conesa, José Javier; Chichón, Francisco Javier; Santiago, César; Sorzano, Carlos Óscar S.; Carrascosa, José L.; Esteban, Mariano

    2015-01-01

    In the HIV vaccine field, there is a need to produce highly immunogenic forms of the Env protein with the capacity to trigger broad B and T-cell responses. Here, we report the generation and characterization of a chimeric HIV-1 gp120 protein (termed gp120-14K) by fusing gp120 from clade B with the vaccinia virus (VACV) 14K oligomeric protein (derived from A27L gene). Stable CHO cell lines expressing HIV-1 gp120-14K protein were generated and the protein purified was characterized by size exclusion chromatography, electron microscopy and binding to anti-Env antibodies. These approaches indicate that gp120-14K protein is oligomeric and reacts with a wide spectrum of HIV-1 neutralizing antibodies. Furthermore, in human monocyte-derived dendritic cells (moDCs), gp120-14K protein upregulates the levels of several proinflammatory cytokines and chemokines associated with Th1 innate immune responses (IL-1β, IFN-γ, IL-6, IL-8, IL-12, RANTES). Moreover, we showed in a murine model, that a heterologous prime/boost immunization protocol consisting of a DNA prime with a plasmid expressing gp120-14K protein followed by a boost with MVA-B [a recombinant modified vaccinia virus Ankara (MVA) expressing HIV-1 gp120, Gag, Pol and Nef antigens from clade B], generates stronger, more polyfunctional, and greater effector memory HIV-1-specific CD4+ and CD8+ T-cell immune responses, than immunization with DNA-gp120/MVA-B. The DNA/MVA protocol was superior to immunization with the combination of protein/MVA and the latter was superior to a prime/boost of MVA/MVA or protein/protein. In addition, these immunization protocols enhanced antibody responses against gp120 of the class IgG2a and IgG3, together favoring a Th1 humoral immune response. These results demonstrate that fusing HIV-1 gp120 with VACV 14K forms an oligomeric protein which is highly antigenic as it activates a Th1 innate immune response in human moDCs, and in vaccinated mice triggers polyfunctional HIV-1-specific adaptive

  8. A poxviral-based cancer vaccine the transcription factor twist inhibits primary tumor growth and metastases in a model of metastatic breast cancer and improves survival in a spontaneous prostate cancer model.

    Science.gov (United States)

    Kwilas, Anna R; Ardiani, Andressa; Dirmeier, Ulrike; Wottawah, Cornelia; Schlom, Jeffery; Hodge, James W

    2015-09-29

    Several transcription factors play a role in the alteration of gene expression that occurs during cancer metastasis. Twist expression has been shown to be associated with the hallmarks of the metastatic process, as well as poor prognosis and drug resistance in many tumor types. However, primarily due to their location within the cell and the lack of a hydrophobic groove required for drug attachment, transcription factors such as Twist are difficult to target with conventional therapies. An alternative therapeutic strategy is a vaccine comprised of a Modified vaccinia Ankara (MVA), incorporating the Twist transgene and a TRIad of COstimulatory Molecules (B7-1, ICAM-1, LFA-3; TRICOM). Here we characterize an MVA-TWIST/TRICOM vaccine that induced both CD4+ and CD8+ Twist-specific T-cell responses in vivo. In addition, administration of this vaccine reduced both the primary tumor growth and metastasis in the 4T1 model of metastatic breast cancer. In the TRAMP transgenic model of spontaneous prostate cancer, MVA-TWIST/TRICOM alone significantly improved survival, and when combined with the androgen receptor antagonist enzalutamide, the vaccine further improved survival. These studies thus provide a rationale for the use of active immunotherapy targeting transcription factors involved in the metastatic process and for the combination of cancer vaccines with androgen deprivation. PMID:26317648

  9. A poxviral-based cancer vaccine targeting the transcription factor Twist inhibits primary tumor growth and metastases in a model of metastatic breast cancer and improves survival in a spontaneous prostate cancer model

    Science.gov (United States)

    Kwilas, Anna R.; Ardiani, Andressa; Dirmeier, Ulrike; Wottawah, Cornelia

    2015-01-01

    Several transcription factors play a role in the alteration of gene expression that occurs during cancer metastasis. Twist expression has been shown to be associated with the hallmarks of the metastatic process, as well as poor prognosis and drug resistance in many tumor types. However, primarily due to their location within the cell and the lack of a hydrophobic groove required for drug attachment, transcription factors such as Twist are difficult to target with conventional therapies. An alternative therapeutic strategy is a vaccine comprised of a Modified vaccinia Ankara (MVA), incorporating the Twist transgene and a TRIad of COstimulatory Molecules (B7-1, ICAM-1, LFA-3; TRICOM). Here we characterize an MVA-TWIST/TRICOM vaccine that induced both CD4+ and CD8+ Twist-specific T-cell responses in vivo. In addition, administration of this vaccine reduced both the primary tumor growth and metastasis in the 4T1 model of metastatic breast cancer. In the TRAMP transgenic model of spontaneous prostate cancer, MVA-TWIST/TRICOM alone significantly improved survival, and when combined with the androgen receptor antagonist enzalutamide, the vaccine further improved survival. These studies thus provide a rationale for the use of active immunotherapy targeting transcription factors involved in the metastatic process and for the combination of cancer vaccines with androgen deprivation. PMID:26317648

  10. Adjuvanting a DNA vaccine with a TLR9 ligand plus Flt3 ligand results in enhanced cellular immunity against the simian immunodeficiency virus.

    Science.gov (United States)

    Kwissa, Marcin; Amara, Rama R; Robinson, Harriet L; Moss, Bernard; Alkan, Sefik; Jabbar, Abdul; Villinger, Francois; Pulendran, Bali

    2007-10-29

    DNA vaccines offer promising strategies for immunization against infections. However, their clinical use requires improvements in immunogenicity. We explored the efficacy of Toll-like receptor (TLR) ligands (TLR-Ls) on augmenting the immunogenicity of a DNA prime-modified vaccinia virus Ankara (MVA) boost vaccine against SIV. Rhesus macaques were injected with Fms-like tyrosine kinase 3 (Flt3)-ligand (FL) to expand dendritic cells (DCs) and were primed with a DNA vaccine encoding immunodeficiency virus antigens mixed with ligands for TLR9 or TLR7/8. Subsequently, the animals were boosted with DNA and twice with recombinant MVA expressing the same antigens. TLR9-L (CpG DNA) mediated activation of DCs in vivo and enhanced the magnitude of antigen-specific CD8(+) interferon (IFN) gamma(+) T cells and polyfunctional CD8(+) T cells producing IFN-gamma, tumor necrosis factor alpha, and interleukin 2. Although this trial was designed primarily as an immunogenicity study, we challenged the animals with pathogenic SIVmac(251) and observed a reduction in peak viremia and cumulative viral loads in the TLR9-L plus FL-adjuvanted group relative to the unvaccinated group; however, the study design precluded comparisons between the adjuvanted groups and the group vaccinated with DNA/MVA alone. Viral loads were inversely correlated with the magnitude and quality of the immune response. Thus, the immunogenicity of DNA vaccines can be augmented with TLR9-L plus FL. PMID:17954572

  11. Enhanced vaccine-induced CD8+ T cell responses to malaria antigen ME-TRAP by fusion to MHC class ii invariant chain.

    Directory of Open Access Journals (Sweden)

    Alexandra J Spencer

    Full Text Available The orthodox role of the invariant chain (CD74; Ii is in antigen presentation to CD4+ T cells, but enhanced CD8+ T cells responses have been reported after vaccination with vectored viral vaccines encoding a fusion of Ii to the antigen of interest. In this study we assessed whether fusion of the malarial antigen, ME-TRAP, to Ii could increase the vaccine-induced CD8+ T cell response. Following single or heterologous prime-boost vaccination of mice with a recombinant chimpanzee adenovirus vector, ChAd63, or recombinant modified vaccinia virus Ankara (MVA, higher frequencies of antigen-specific CD4+ and CD8+ T cells were observed, with the largest increases observed following a ChAd63-MVA heterologous prime-boost regimen. Studies in non-human primates confirmed the ability of Ii-fusion to augment the T cell response, where a 4-fold increase was maintained up to 11 weeks after the MVA boost. Of the numerous different approaches explored to increase vectored vaccine induced immunogenicity over the years, fusion to the invariant chain showed a consistent enhancement in CD8+ T cell responses across different animal species and may therefore find application in the development of vaccines against human malaria and other diseases where high levels of cell-mediated immunity are required.

  12. Nanoparticle-based targeting of vaccine compounds to skin antigen-presenting cells by hair follicles and their transport in mice.

    Science.gov (United States)

    Mahe, Brice; Vogt, Annika; Liard, Christelle; Duffy, Darragh; Abadie, Valérie; Bonduelle, Olivia; Boissonnas, Alexandre; Sterry, Wolfram; Verrier, Bernard; Blume-Peytavi, Ulrike; Combadiere, Behazine

    2009-05-01

    Particle-based drug delivery systems target active compounds to the hair follicle and may result in a better penetration and higher efficiency of compound uptake by skin resident cells. As previously proposed, such delivery systems could be important tools for vaccine delivery. In this study, we investigated the penetration of solid fluorescent 40 or 200 nm polystyrene nanoparticles (NPs) as well as virus particles in murine skin to further investigate the efficacy of transcutaneously (TC) applied particulate vaccine delivery route. We demonstrated that 40 and 200 nm NPs and modified vaccinia Ankara (MVA) expressing the green-fluorescent protein penetrated deeply into hair follicles and were internalized by perifollicular antigen-presenting cells (APCs). Fibered-based confocal microscopy analyses allowed visualizing in vivo particle penetration along the follicular duct, diffusion into the surrounding tissue, uptake by APCs and transport to the draining lymph nodes. The application of small particles, such as ovalbumin coding DNA or MVA, induced both humoral and cellular immune responses. Furthermore, TC applied MVA induced protection against vaccinia virus challenge. Our results strengthen the concept of TC targeting of cutaneous APCs by hair follicles and will contribute to the development of advanced vaccination protocols using NPs or viral vectors. PMID:19052565

  13. Multiserotype protection elicited by a combinatorial prime-boost vaccination strategy against bluetongue virus.

    Directory of Open Access Journals (Sweden)

    Eva Calvo-Pinilla

    Full Text Available Bluetongue virus (BTV belongs to the genus Orbivirus within the family Reoviridae. The development of vector-based vaccines expressing conserved protective antigens results in increased immune activation and could reduce the number of multiserotype vaccinations required, therefore providing a cost-effective product. Recent recombinant DNA technology has allowed the development of novel strategies to develop marker and safe vaccines against BTV. We have now engineered naked DNAs and recombinant modified vaccinia virus Ankara (rMVA expressing VP2, VP7 and NS1 proteins from BTV-4. IFNAR((-/- mice inoculated with DNA/rMVA-VP2,-VP7-NS1 in an heterologous prime boost vaccination strategy generated significant levels of antibodies specific of VP2, VP7, and NS1, including those with neutralizing activity against BTV-4. In addition, vaccination stimulated specific CD8(+ T cell responses against these three BTV proteins. Importantly, the vaccine combination expressing NS1, VP2 and VP7 proteins of BTV-4, elicited sterile protection against a lethal dose of homologous BTV-4 infection. Remarkably, the vaccine induced cross-protection against lethal doses of heterologous BTV-8 and BTV-1 suggesting that the DNA/rMVA-VP2,-VP7,-NS1 marker vaccine is a promising multiserotype vaccine against BTV.

  14. Prevalence of orthorexia nervosa in resident medical doctors in the faculty of medicine (Ankara, Turkey).

    Science.gov (United States)

    Bağci Bosi, A Tülay; Camur, Derya; Güler, Cağatay

    2007-11-01

    This study has been carried out to "identify highly sensitive behavior on healthy nutrition (orthorexia nervosa-ON)" in residence medical doctors (MD) in the Faculty of Medicine. Diagnoses of ON was based on the presence of a disorder with obsessive-compulsive personality. The study is a cross-sectional research, which reached out to the entire 318 MD. The ORTO-15 test was used to propose a diagnostic proceeding and to try verify the prevalence of ON. Those subjects who were classified below 40 from the ORTO-15 test are accepted to have ON. Chi-square test, ANOVA (univariate) analysis and logistic regression were used for analyses of the data. Mean score of the participants from the ORTO-15 test is 39.8+/-0.22, and there is no statistical difference between women and men. A total of 45.5% of the residence MD involved in the research scored below 40 in the ORTO-15 test. Those who do their food shopping themselves, skip a meal with a salad/fruit, care about the quality of the things they eat, think that eating outside is healthy, look at the content of what they eat and the content of food is important in selection of a product score lower in their average marks in ORTO-15 and the difference among the groups is statistically significant. Food selection of 20.1% of the male participants and 38.9% of the female participants among the residence MD is influenced by the programs on nutrition/health in mass-media. The difference between the groups is statistically significant (p<0.05). Female medical doctors are more careful than men of their physical appearance and weight control and consume less caloric food, which is statistically significant. Since those who exhibit "healthy fanatic" eating habits may have a risk of ON in the future, it would be useful to conduct studies that identify the prevalence of ON in the public. PMID:17586085

  15. Occupational accidents and affecting factors of metal industry in a factory in Ankara

    Directory of Open Access Journals (Sweden)

    Mustafa N. Ilhan

    2012-08-01

    Full Text Available Abstract Objective:According to the statistics of the Social Security Institution, 18672 occupational accidents occurred in the metal industry in 2008 in Turkey. Whereas 78 of these accidents resulted in death, 252 people became permanently incapable of working. In 2008, 369677 working days were lost as a result of occupational accidents. Evaluating the reasons for and the results of accidents in the metal industry and contributing to the development of recommendations for prevention in accordance with the information obtained. Method: The study was conducted with 201 of 210 workers working in heavy metal manufacturing and construction in the building company between April 2008 and June 2008. Results: The frequency of occupational accidents among the metal workers was 22% between January 2007 and June 2008. The reasons for the workers’ accidents are listed as; insufficient use of personal protective equipment (44%, carelessness (37%, and personal reasons, not to be taken of security measures at machines and looms/ unsuitable machines (both 17%.Conclusion: The study demonstrates that the accidents mostly occur because of failure to use of personal protective equipment, insufficient vocational training. Key Words: Occupational, accident, metal industry, preventionAnkara’da bir metal sanayi fabrikasında iş kazaları ve etkileyen faktörler Özet Amaç: 2008 yılında Sosyal Güvenlik Kurumu’nun verilerine göre metal sanayisinde 18672 iş kazası meydana gelmiş ve 369677 işgünü kaybı olmuştur.  Bu kazalardan 78 tanesi ölümle sonuçlanırken, 252 kişi kalıcı olarak işgöremez hale gelmiştir. Metal sanayisinde meydana gelen kazaların sebep ve sonuçlarını inceleyerek, elde edilen bilgiler doğrultusunda kazaların önlenmesine yönelik tavsiyelerin geliştirilmesi amaçlanmıştır. Yöntem: Araştırma, Ankara’da faaliyet gösteren ağır metal imalat, konstrüksiyon ve inşaat sanayi şirketinde 2008 Nisan-2008 Haziran döneminde çalışan 210 işçinin 201’ine anket uygulanmasıyla yürütülmüştür. Bulgular: Ocak 2007 ve Haziran 2008 tarihleri arasında metal işçilerinin iş kazası sıklığı %22 bulunmuştur. İş kazalarının nedenleri olarak yetersiz kişsel koruyucu ekipman kullanımı (%44, dikkatsizlik (%37, kişisel nedenler (%17 ile makine ve tezgahlarda güvenlik önlemlerinin alınmaması/makinelerin uygun olmaması (%17 belirtilmiştir. Sonuç: Çalışma, iş kazalarının çoğunlukla kişisel koruyucu ekipmanın kullanımındaki eksiklikten ve yetersiz mesleki eğitimden kaynaklandığını ortaya koymuştur. Anahtar Kelimeler: İş, kaza, metal sanayi, önleme 

  16. Consumer Decision - Making Process in E - Commerce: Case of Salzburg - Ankara

    OpenAIRE

    Ahmet Gürbüz; Nurettin Ayaz; Mete Albayrak

    2015-01-01

    In a globalized world, companies need to track the changes occur in their internal and external stakeholders in order to develop consistent strategies, structures and systems for success in e-commerce. Tracking and researching the existing and potential customers and companies as external stakeholders has become very important. This study aims to reveal a scientific approach about the purchasing decisions of e-commerce companies’ customers, to carry the...

  17. Characteristics of Children with Acute Carbon Monoxide Poisoning in Ankara: A Single Centre Experience.

    Science.gov (United States)

    Unsal Sac, Rukiye; Taşar, Medine Ayşin; Bostancı, İlknur; Şimşek, Yurda; Bilge Dallar, Yıldız

    2015-12-01

    The purpose of the study was to define characteristics of children with acute carbon monoxide poisoning. Eighty children hospitalized with acute carbon monoxide poisoning were recruited prospectively over a period of 12 months. Sociodemographic features, complaints and laboratory data were recorded. When the patient was discharged, necessary preventive measures to be taken were explained to parents. One month later, the parents were questioned during a control examination regarding the precautions that they took. The ages of the cases were between one month and 16 yr. Education levels were low in 86.2% of mothers and 52.6% of fathers. All families had low income and 48.8% did not have formal housing. The source of the acute carbon monoxide poisoning was stoves in 71.2% of cases and hot-water heaters in 28.8% of cases. Three or more people were poisoned at home in 85.1% of the cases. The most frequent symptoms of poisoning were headache and vertigo (58.8%). Median carboxyhemoglobin levels at admission to the hospital and discharge were measured as 19.5% and 1.1% (P carbon monoxide poisoning are usually from families with low socioeconomic and education levels. Education about prevention should be provided to all people who are at risk of carbon monoxide poisoning before a poisoning incident occurs. PMID:26713060

  18. University Students' Perceptions of Childless Couples and Parents in Ankara, Turkey

    Science.gov (United States)

    Copur, Zeynep; Koropeckyj-Cox, Tanya

    2010-01-01

    Changing perceptions of childlessness have been documented in the United States, but little is known about perceptions in developing countries undergoing rapid social changes and globalization, including Turkey. This project uses a survey and hypothetical vignettes about childless couples and parents to assess university students' perceptions of…

  19. 76 FR 537 - Eurasian Oil and Gas Suppliers Mission to Almaty, Kazakhstan Ankara and Istanbul Turkey

    Science.gov (United States)

    2011-01-05

    ... capacity by 2019, to over 1.3 million BPD. Turkey's oil and gas market provides excellent opportunities for... experts and the U.S. Commercial Service in Almaty estimate that the current market for oil and gas field... Eurasian Region oil and gas equipment and services market Applicant's potential for business in Turkey...

  20. Breast milk lead and cadmium levels from suburban areas of Ankara

    Energy Technology Data Exchange (ETDEWEB)

    Oruen, Emel, E-mail: emelorun@hotmail.com [Department of Pediatrics, Fatih University Hospital, Ankara (Turkey); Yalcin, S. Songuel, E-mail: siyalcin@hacettepe.edu.tr [Social Pediatric Unit, Hacettepe University Ihsan Dogramaci Children Hospital, Ankara (Turkey); Aykut, Osman; Orhan, Guennur; Morgil, Goeksel Koc [Analytic Toxicology Laboratory, Refik Saydam National Public Health Agency, Ankara (Turkey); Yurdakoek, Kadriye [Social Pediatric Unit, Hacettepe University Ihsan Dogramaci Children Hospital, Ankara (Turkey); Uzun, Ramazan [Analytic Toxicology Laboratory, Refik Saydam National Public Health Agency, Ankara (Turkey)

    2011-06-01

    The objectives of this study were (1) to evaluate levels of lead (Pb) and cadmium (Cd) in the breast milk at 2 months postpartum, (2) to investigate the relationship between Pb and Cd levels in breast milk and some sociodemographic parameters and (3) to detect whether these levels have any influence on the infant's physical status or on postpartum depression in the mothers. Pb and Cd levels in breast milk were determined by Inductively Coupled Plasma Mass Spectroscopy (ICP-MS). The median breast milk concentrations of Pb and Cd were 20.59 and 0.67 {mu}g/l, respectively. In 125 (87%) of 144 samples, Pb levels were higher than the limit in breast milk reported by the World Health Organization (WHO) (> 5 {mu}g/l). Breast milk Cd levels were > 1 {mu}g/l in 52 (36%) mothers. The mothers with a history of anemia at any time had higher breast milk Pb levels than those without a history of anemia (21.1 versus 17.9 {mu}g/l; p = 0.0052). The median breast milk Cd levels in active and passive smokers during pregnancy were significantly higher than in non-smokers (0.89, 0.00 {mu}g/l, respectively; p = 0.023). The breast milk Cd levels of the mothers who did not use iron and vitamin supplements for 2 months postpartum were found to be higher than in those who did use the supplements (iron: 0.73, 0.00 {mu}g/l, p = 0.023; vitamin: 0.78, 0.00 {mu}g/l, p = 0.004, respectively). Breast milk Cd levels at the 2nd month were correlated negatively with the z scores of head circumference and the weight for age at birth (r = - 0.257, p = 0.041 and r = - 0.251, p = 0.026, respectively) in girls. We found no correlation between the breast milk Pb and Cd levels and the Edinburgh Postpartum Depression Scale scores. Breast milk monitoring programs should be conducted that have tested considerable numbers of women over time in view of the high levels of Pb in breast milk in this study. - Research highlights: {yields} Breast milk Pb levels were higher than the advised safety limits. {yields} The mothers having history of anemia have higher Pb levels than those of have not. {yields} Breast milk Cd levels in non-smokers were lower than others. {yields} Breast milk Cd level was negatively correlated with HCZ and WAZ at birth in girls.

  1. Strategies for Promoting Enterpreneurship in Local Economic Development: Case of Ankara-Turkey

    OpenAIRE

    Çiğdem VAROL

    2010-01-01

    Urban areas are complex and dynamic spaces. They reflect many processes of physical, social, environmental and economic transition. As they are the main sources of  employment, negative changes in economic structure have direct impacts upon the rise of unemployment,  impoverishment, thus social stress in cities. Not only the developing countries but also the advanced countries have  been experiencing economic recession, thus the central or local governments try to develop diffe...

  2. Breast milk lead and cadmium levels from suburban areas of Ankara

    International Nuclear Information System (INIS)

    The objectives of this study were (1) to evaluate levels of lead (Pb) and cadmium (Cd) in the breast milk at 2 months postpartum, (2) to investigate the relationship between Pb and Cd levels in breast milk and some sociodemographic parameters and (3) to detect whether these levels have any influence on the infant's physical status or on postpartum depression in the mothers. Pb and Cd levels in breast milk were determined by Inductively Coupled Plasma Mass Spectroscopy (ICP-MS). The median breast milk concentrations of Pb and Cd were 20.59 and 0.67 μg/l, respectively. In 125 (87%) of 144 samples, Pb levels were higher than the limit in breast milk reported by the World Health Organization (WHO) (> 5 μg/l). Breast milk Cd levels were > 1 μg/l in 52 (36%) mothers. The mothers with a history of anemia at any time had higher breast milk Pb levels than those without a history of anemia (21.1 versus 17.9 μg/l; p = 0.0052). The median breast milk Cd levels in active and passive smokers during pregnancy were significantly higher than in non-smokers (0.89, 0.00 μg/l, respectively; p = 0.023). The breast milk Cd levels of the mothers who did not use iron and vitamin supplements for 2 months postpartum were found to be higher than in those who did use the supplements (iron: 0.73, 0.00 μg/l, p = 0.023; vitamin: 0.78, 0.00 μg/l, p = 0.004, respectively). Breast milk Cd levels at the 2nd month were correlated negatively with the z scores of head circumference and the weight for age at birth (r = - 0.257, p = 0.041 and r = - 0.251, p = 0.026, respectively) in girls. We found no correlation between the breast milk Pb and Cd levels and the Edinburgh Postpartum Depression Scale scores. Breast milk monitoring programs should be conducted that have tested considerable numbers of women over time in view of the high levels of Pb in breast milk in this study. - Research highlights: → Breast milk Pb levels were higher than the advised safety limits. → The mothers having history of anemia have higher Pb levels than those of have not. → Breast milk Cd levels in non-smokers were lower than others. → Breast milk Cd level was negatively correlated with HCZ and WAZ at birth in girls.

  3. [Cryptosporidiosis in newborn calves in Ankara region: clinical, haematological findings and treatment with Lasalocid-NA].

    Science.gov (United States)

    Sahal, M; Karaer, Z; Yasa Duru, S; Cizmeci, S; Tanyel, B

    2005-06-01

    In the first part of the present study, a total of 109 faeces samples collected from calves suffering from diarrhoea were examined for Cryptosporidium parvum oocytes and 39 (35.8%) of them were found to be positive. On the basis of oocyte counts, 14 (36%) samples were assessed as mildly infected and 25 (64%) samples as heavily infected. The occurrence of the disease was more common in winter (56.4%) than during other seasons (autuma 0%, summer 15.4% spring 28.2%. In the present study, the ionophore polyetherantibiotic Lasalocid-Na, that is licensed as a feed additive (Bovatec, 15% Lasalocid-Na, Roche AG) in Turkey, was administered to 11 calves naturally infected with Cryptosporidium and its therapeutic effect was evaluated. Lasalocid-Na (8 mg/ kg BW) was given once daily for 3 days added to the milk. The clinical parameters of infected calves were evaluated before and 3 days after the treatment in 24 hour intervals. The oocyst counts of faeces of calves with cryptosporidiosis were between 15 x 10(6) and 96 x 10(6)/mL before treatment. No oocystes were found in faecal samples of 3 calves (27.3%) after 48 hours and 4 (40%) calves after 72 hours of treatment, respectively. The number of oocytes in the faeces of the remaining calves varied between 90 and 1.2 x 10(6)/mL during the respective period. The number of oocystes before treatment was significantly higher than the number of oocytes after treatment. One of the calves died 56 h after the first treatment despite the treatment. The pH of venous blood was decreased prior to treatment as expected. The lowest pH was 6.83, the lowest bicarbonate concentration was 3.80 mmol/l and the lowest base excess was -31.2 mmol/l. After the treatment, pH, pCO2, HCO3- and BE values of the venous blood increased significantly and reached physiological values before discharge. The differences between the values assessed before the treatment and at the 2nd, 3rd, and 4th sampling time were statistically significant. Number of leucocyte and haemoglobin concentration before the treatment were significantly higher than the values after treatment (p intoxication symptoms in respect to present administration form and dosage were found only in one calf (9%). Treatment procedure in the present study with Lasalocid-Na was found to be suitable in combination with an adequate infusion therapy for the treatment of calves on farms with problems related to cryptosporidiosis. Although the use of Lasolacid-Na for the treatment of Cryptosporidium infection in the EU is banned, it might be used as an alternative drug outside of the EU since it has a successful effect for preventing reinfections. PMID:16028484

  4. Quality assurance and quality control of nuclear analytical laboratories in Ankara nuclear research and training center

    International Nuclear Information System (INIS)

    The objective of this project is to introduce quality assurance systems for validated analytical data in nuclear laboratories of Center.In trade,health,safety and environmental protection, the users of a laboratory's analytical results are increasingly requiring demostrable proof of the reliability and credibility of the results using internationally accepted standards. Such demands are being imposed by the European Community. In addition to this, there is growing need for laboratories to operate efficiently and effectively to reduce internal waste to provide reliable and verifiable reports in a timely and economical manner.International Atomic Energy Agency assist laboratories to improve their QA activities to a level of performance which satisfies the requirements of the immediate beneficiaries and ultimately to a level of certification. A comprehensive QA/QC programme is applied to NALs - ANAEM which the QA system is self- sustainable for official accreditation

  5. An Analyses of Bicycle Accidents in Ankara: Analyses of 5 Years

    Directory of Open Access Journals (Sweden)

    Muhittin Yilmaz

    2014-12-01

    Full Text Available Aim: Bicycles are used for transportation, exercise and recreation. In this study we aimed to investigate the epidemiological, clinical and economic aspects of bicycle injuries. Material and Method: We included in the study who admitted to the Emergency Department with a bicycle accident between January 2008 and July 2012. Patient age and sex, accident pattern, presence of personal protective measures, injured body part, Glasgow Coma Scale (GCS score, Revised Trauma Score (RTS, radiological findings, requested consultations, duration of hospital stay, season of injury, and average cost rates were recorded. The study population was divided into 2 age groups as 0-14 years and over 14 years. Results: A patients were 238 (81.2% male and their mean age was 31.5±14.1 years. Fifty-five (18.8% patients were female and their mean age was 34.5±15.9 years. Males had significantly higher rate of bicycle injury (p

  6. THE SCREENING OF DEPRESSION SYMPTOMS OF THE ELDERLY LIVING IN A RESIDENTIAL HOME IN ANKARA

    Directory of Open Access Journals (Sweden)

    Sema ATTILA

    2006-10-01

    Full Text Available The study was conducted with the participation of elderly living in Kecioren Municipality Residential Home to determine their sociodemographic properties, percentage of depression risk, and opinion about the institution. The universe of the study was composed of 117 elderly living in Kecioren Municipality Residential Home. Eighty seven elderly (74.4% participated in the study. It was a descriptive study. Geriatric Depression Scale (GDS score, sociodemographic properties, presence of chronic disease, mental health and opinion about the institution were the variables. Face to face interview technique was used. The data was collected with a 27 item questionnaire and 30 item GDS questionnaire. The average age of the elderly was 76.1 (standart deviation=8.7. Women composed 63.4% (52 persons of the elderly. Of the participants, 63.4% (52 persons felt themselves as very healthy or healthy. According to GDS score, 41.5% of the elderly, living in residential home, had depression risk . There was a statistically significant relation between feeling himself/herself healthy and GDS score. As 41.5% of the elderly had GDS score of 14 or more, it would be useful to improve psychologic counselling services at the institution and apply screening tests rutinly. [TAF Prev Med Bull 2006; 5(5.000: 332-342

  7. Pattern of acute poisonings in childhood in Ankara: what has changed in twenty years?

    Science.gov (United States)

    Andiran, Nesibe; Sarikayalar, Fikriye

    2004-01-01

    Poisoning represents one of the most common medical emergencies in childhood, and epidemiological properties differ from country to country. Thus, special epidemiological surveillance for each country is necessary to determine the problem according to which preventive measures can be taken. The purpose of this study was to clarify the characteristics of acute poisoning cases admitted to a pediatric referral hospital. All poisoned patients under 17 years of age, except for cases food poisoning, presenting to the Emergency Department (ED) from January 1995 to December 2000 were determined. The information about each case was recorded on standardized forms and a retrospective chart review survey was done. Complete epidemiological and clinical data were obtained for 489 patients. The mean age of all poisoned patients (mean +/- standard deviation) was 5.96 +/- 4.87 years, and the age range was 0.01 to 17 years. Three hundred and thirty-one children, forming 63.6% of all patients, were under five years of age. Slightly more boys (52.3%) than girls were intoxicated at ages less than 10 years, after which more girls (79%) than boys were involved. The majority of all cases were due to accidental poisoning (78.1% of all poisonings) which occurred mostly in children under five years of age (73.3%). While accidental poisonings (97.1%) were the most common mode of poisoning between 1-5 years, self-poisonings (67.3%) had the highest ratio in cases over 10 years of age. In patients younger than one year of age, 74.2% of all poisonings were due to therapeutical error. Drugs were the most frequent offending agent (57.7%), followed by ingestion of a caustic/corrosive substance (16.8%) and carbon monoxide (CO) intoxication (9.4%). Analgesics were the most common agents, forming 23.7% of all poisonings due to drugs, followed by ingestion of multiple drugs and tricyclic antidepressants at ratios of 21.6% and 9.6%, respectively. The most common route of poisoning was ingestion of the poison (437/489 patients, 89.4%) and most were ingested inside the house (93.3%). About half of all poisoned patients (50.9%) were admitted to the ED within the first two hours of ingestion, and gastric lavage was performed on about half of the poisoned children (48.7%). In most of the cases, hospital treatment was non-specific, including general measures of decontamination and supportive-symptomatic therapy. During the six-year study period, two patients were lost due to acute poisoning, yielding an overall mortality rate of 0.4%. While most of the poisonings were due to accidental ingestions in infancy and primary school ages without sex predilection, the incidence of self-poisonings, especially in girls, was found to be increased. Analgesics, tricyclic antidepressant drugs (which seemed to form a new and dangerous group) and caustic/corrosive substances were the most commonly ingested agents. The early awareness of poisoning and appropriate therapeutic measures taken seemed to be efficacious with a very low mortality rate. The epidemiological and preventive properties of childhood poisonings should be further searched by prospectively designed multicentered studies throughout our country. PMID:15214744

  8. On-going research projects at Ankara Nuclear Research Center in agriculture and animal science

    International Nuclear Information System (INIS)

    The projects in progress conducted by the Center comprise nuclear-aided researches in soil fertility, plant nutrition, plant protection, improvement of field crops, improvement of horticultural plants and forest trees by mutation breeding, in vitro culture technique with mutagen treatments, use of phosphogypsum in soil amelioration, sterilization of medical supplies, wastewater treatment, animal nutrition, animal health and productivity and accreditation. The on-going main projects involving several sub-projects with the above subjects were summarized for possible future collaborations. (author)

  9. Human cytomegalovirus vaccine based on the envelope gH/gL pentamer complex.

    Science.gov (United States)

    Wussow, Felix; Chiuppesi, Flavia; Martinez, Joy; Campo, John; Johnson, Erica; Flechsig, Christin; Newell, Maegan; Tran, Elaine; Ortiz, Jose; La Rosa, Corinna; Herrmann, Andreas; Longmate, Jeff; Chakraborty, Rana; Barry, Peter A; Diamond, Don J

    2014-11-01

    Human Cytomegalovirus (HCMV) utilizes two different pathways for host cell entry. HCMV entry into fibroblasts requires glycoproteins gB and gH/gL, whereas HCMV entry into epithelial and endothelial cells (EC) requires an additional complex composed of gH, gL, UL128, UL130, and UL131A, referred to as the gH/gL-pentamer complex (gH/gL-PC). While there are no established correlates of protection against HCMV, antibodies are thought to be important in controlling infection. Neutralizing antibodies (NAb) that prevent gH/gL-PC mediated entry into EC are candidates to be assessed for in vivo protective function. However, these potent NAb are predominantly directed against conformational epitopes derived from the assembled gH/gL-PC. To address these concerns, we constructed Modified Vaccinia Ankara (MVA) viruses co-expressing all five gH/gL-PC subunits (MVA-gH/gL-PC), subsets of gH/gL-PC subunits (gH/gL or UL128/UL130/UL131A), or the gB subunit from HCMV strain TB40/E. We provide evidence for cell surface expression and assembly of complexes expressing full-length gH or gB, or their secretion when the corresponding transmembrane domains are deleted. Mice or rhesus macaques (RM) were vaccinated three times with MVA recombinants and serum NAb titers that prevented 50% infection of human EC or fibroblasts by HCMV TB40/E were determined. NAb responses induced by MVA-gH/gL-PC blocked HCMV infection of EC with potencies that were two orders of magnitude greater than those induced by MVA expressing gH/gL, UL128-UL131A, or gB. In addition, MVA-gH/gL-PC induced NAb responses that were durable and efficacious to prevent HCMV infection of Hofbauer macrophages, a fetal-derived cell localized within the placenta. NAb were also detectable in saliva of vaccinated RM and reached serum peak levels comparable to NAb titers found in HCMV hyperimmune globulins. This vaccine based on a translational poxvirus platform co-delivers all five HCMV gH/gL-PC subunits to achieve robust humoral

  10. MATHEMATICAL ANALYSIS OF PRODUCTION QUOTA FOR 30 MVA ELECTRIC MANGANESE SILICON ALLOY FURNACE%30MVA 锰硅合金炉生产指标数理分析

    Institute of Scientific and Technical Information of China (English)

    陈建武

    2000-01-01

    根据30MVA锰硅合金电炉实际生产数据,利用多元线性回归方程与高斯最小二乘法,分析了二次电压、二次电流、三元碱度、硅渣配加量、出铁次数对生产综合指标的影响情况,通过分析,分清了影响因素的主次,并据此提出了电炉的合理操作建议.

  11. Síntesis y estudios de caracterización preliminares de nuevos materiales Sonogel modificados con Nanocarbon

    OpenAIRE

    García Romero, Magdalena

    2015-01-01

    Los materiales sol-gel comenzaron a tomar protagonismo a mediados del s.XVIII con los estudios de Ebelman y Graham. Posteriormente, estos han sido ampliados por numerosos grupos de investigación, entre los cuales se encuentra el grupo de Instrumentación y Ciencias Ambientales (FQM- 249) del Departamento de Química Analítica de la Universidad de Cádiz, donde se han realizado diversos estudios sobre el Sonogel y el Sonogel-Carbono. La química del sol-gel proporciona un medio para...

  12. Eletrodos modificados com polímeros perfluorados e sulfonados: aplicações em análises ambientais

    Directory of Open Access Journals (Sweden)

    Fungaro Denise Alves

    2000-01-01

    Full Text Available Perfluoro and sulfonated ion-exchange polymers are recognized as a very useful material for various mechanistic studies and applications in electrochemistry. These polymers are characterized by high equivalent weights and by a low number of ion-exchange sites interposed between long organic chains. The solubility enables a preparation of stable polyelectrolyte films on the electrode surface. Examples of the determination of trace metals and organic componds in real environmental samples are presented.

  13. Comportamiento frente a la durabilidad de morteros de reparación de cemento modificados con polímeros

    OpenAIRE

    González Lucas, Ángel

    2014-01-01

    El auge que ha surgido en los últimos años por la reparación de edificios y estructuras construidas con hormigón ha llevado al desarrollo de morteros de reparación cada vez más tecnológicos. En el desarrollo de estos morteros por parte de los fabricantes, surge la disyuntiva en el uso de los polímeros en sus formulaciones, por no encontrarse justificado en ocasiones el trinomio prestaciones/precio/aplicación. En esta tesis se ha realizado un estudio exhaustivo para la justificación de la ...

  14. Aplicación de la técnica PRAT Modificado en odontología

    OpenAIRE

    Alfaro, Martín Rodrigo; Alfaro, Gabriel Enrique; Bentivegna, Nicolás; Amaro, Emilio Gustavo; Lazo, Gabriel Eduardo; Lazo, Sergio Daniel

    2015-01-01

    Introducción: Paciente de 8 años de Edad, de sexo femenino, residente de la localidad de Fortín Soledad, Provincia de Formosa, Argentina. En el marco del proyecto de extensión universitaria “Formosa Salud Bucal” del Voluntariado Universitario de la Prosecretaria de Acción Comunitaria perteneciente a la Secretaria de Planificación y Extensión Universitaria Facultad de Odontología de la UNLP. Presenta una lesión de surco profundo cariado en la pieza 46 sin presentar sintomatología alguna. Descr...

  15. Síntese de poliuretanos modificados por óleo de mamona empregados em materiais energéticos

    Directory of Open Access Journals (Sweden)

    Roberta Jachura Rocha

    2013-01-01

    Full Text Available The aim of this work was to synthesize a polyurethane polymer matrix using castor oil as a polymer chain modifier, whose characteristics can be adjusted for use as a binder in the manufacture of energetic materials such as propellant and pyrotechnics for aerospace use. We attempted the partial substitution of hydroxyl-terminated polybutadiene (HTPB, a pre-polymer commonly used as a starting polyol in obtaining energetic matrix composites. Thermoanalytical techniques were employed to characterize the material based on castor oil and the unmodified HTPB. The results showed similar behaviors, confirming the possibility of their use as polymer matrix composites through the proposed adaptations.

  16. Síntese de poliuretanos modificados por óleo de mamona empregados em materiais energéticos

    OpenAIRE

    Roberta Jachura Rocha; José Eduardo Salgueiro Lima; Susane Ribeiro Gomes; Koshun Iha; José Atílio Fritz Fidel Rocco

    2013-01-01

    The aim of this work was to synthesize a polyurethane polymer matrix using castor oil as a polymer chain modifier, whose characteristics can be adjusted for use as a binder in the manufacture of energetic materials such as propellant and pyrotechnics for aerospace use. We attempted the partial substitution of hydroxyl-terminated polybutadiene (HTPB), a pre-polymer commonly used as a starting polyol in obtaining energetic matrix composites. Thermoanalytical techniques were employed to characte...

  17. Alimentos funcionales de origen animal con perfil lipídico modificado: estrategia, herramientas y diseño

    OpenAIRE

    Martínez Ruiz, Beatriz

    2011-01-01

    In recent years the interest of the food industries and the consumers by the concept of "functional food" has been increased. Due to the growing interest by the consumers for foods health, the food industry had understood the potentiality of functional foods` market. Consequently, these companies had been initiated an intense research activity in the area of functional foods all over the world. On the other hand, it should be emphasized that dietary fat is the nutrient whose has received m...

  18. Nano mechanical properties of carbon films modified by ion radiation; Propriedades nanomecanicas de filmes de carbono modificados por irradiacao ionica

    Energy Technology Data Exchange (ETDEWEB)

    Foerster, C.E.; Serbena, F.C. [Universidade Federal do Parana (UFPR), Curitiba, PR (Brazil). Dept. de Fisica; Lepienski, C.M.; Odo, G.Y. [Universidade Estadual de Ponta Grossa, PR (Brazil). Dept. de Fisica; Zawislak, F.C.; Lopes, J.M.J.; Baptista, D.L.; Garcia, I.T.S. [Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS (Brazil). Inst. de Fisica

    2000-07-01

    In present work it is measured hardness, Young modulus and friction coefficient values for different types of carbon films. These films were submitted to different ion bombardment conditions (energy and fluencies). The mechanical behavior was obtained by nano indentation technique and analyzed by the Oliver/Pharr method. For friction coefficient determination the nano scratch procedure is used. Pristine C{sub 60} films (fullerenes) has a hardness of 0.33 GPa. After irradiation with different ions (He, N and Bi), the hardness raise to about 14 GPa and the Young modulus change from 20 to about 200 GPa. For photoresist film AZ-1350J irradiation with Ar and He change the hardness from 0.4 to about 14 GPa and the Young modulus raise from 4 to 80 GPa. In a-C-H the hardness change from 3.5 to 11 GPa when submitted to N irradiation. In PPA films the hardness value raise from 0.5 to 11 GPa after irradiation with Ar. These mechanical and tribological results were analyzed in terms of deposited energy by the ion irradiation and compared with those presented in the literature. (author)

  19. Adhesivos tipo poliuretano obtenidos a partir de aceite de ricino y almidón químicamente modificados

    Directory of Open Access Journals (Sweden)

    Manuel F. Valero

    2013-01-01

    Full Text Available This study reports the preparation of polyurethane adhesives using polyols obtained from castor oil modified by a transesterification reaction with pentaerythritol and starch modified by glycosylation. The physical properties of the polyols such as hydroxyl value were determined and the infrared spectroscopic analysis of the polyols reported. The effect of varying the hydroxyl value in the polyols on physical properties of polyurethane coatings on wood and steel panels was determined. The characterization of polyurethane coatings carried out by IR spectroscopic analysis, scratch hardness resistance, impact resistance, lap shear strength, T-peel strength measurements, solvent resistance and chemical resistant determination were reported.

  20. Adhesivos tipo poliuretano obtenidos a partir de aceite de ricino y almidón químicamente modificados

    OpenAIRE

    Manuel F. Valero

    2013-01-01

    This study reports the preparation of polyurethane adhesives using polyols obtained from castor oil modified by a transesterification reaction with pentaerythritol and starch modified by glycosylation. The physical properties of the polyols such as hydroxyl value were determined and the infrared spectroscopic analysis of the polyols reported. The effect of varying the hydroxyl value in the polyols on physical properties of polyurethane coatings on wood and steel panels was determined. The cha...

  1. DISPOSITIVOS DGT MODIFICADOS COM MATERIAIS ALTERNATIVOS PARA USO NA ESPECIAÇÃO DE ELEMENTOS TRAÇO

    Directory of Open Access Journals (Sweden)

    Cristiano L. Chostak

    2015-03-01

    Full Text Available The use of MT-K10 Montmorillonite immobilized onto agarose was investigated in this work as an alternative binding phase in Diffusive Gradient in Thin Film (DGT devices for the determination of metallic labile species. In addition, agarose itself was also used as the diffusive phase. The percentage of sorption of Zn2+, Cu2+, Cr3+, Mn2+, Cd2+, Pb2+, and Ni2+ onto the binding phase was higher than 80% and the desorption process for all elements was also greater than 75%. Elution factors were determined experimentally, ranging from 0.74 for Zn2+ and 0.90 for Cr3+ and Pb2+. The accumulation of all species was linear with time, in agreement with the Fick's 1st law of diffusion. The deployment of the alternative devices in natural waters was compared to commercial devices. Labile concentrations determined by the alternative devices were slightly superior compared to results obtained with the deployment of original DGT devices due to the less restrictive pores of agarose.

  2. Pengaruh Mikoriza Vesikula Arbuskula (MVA) dan Pengunaan Mulsa Terhadap Pertumbuhan dan Produksi Kacang Hijau (Vigna radiata L. )

    OpenAIRE

    Sirait, Rajani

    2011-01-01

    The research was to examined the effect of vesicular arbuscular micorrhizae and by using mulch for the growth and production of mung bean. The experiment has done at Faculty of Agriculture, University of Sumatera Utara, Medan. The experiment used Randomized Block Design (RDB) Factorial with two treatment factors and three replication. First factor was vesicular arbuscular michorrrizae with four level, 0 g/plant, 4 g/plant, 8 g/plant, 12 g/plant. Second factor was mulch with thr...

  3. In vivo evaluation of recombinant Vaccinia virus MVA delivering ancestral H9 hemagglutinin antigen of Avian Influenza virus

    OpenAIRE

    Becker, Jens Michael

    2015-01-01

    Avian Influenza (AI) viruses pose a threat to human and animal health and are responsible for potential economic losses. From the waterfowl reservoir, these RNA viruses can be transmitted to domestic poultry and humans, causing illness and death among people as well as mass culling of farm birds worldwide. This study contributes to increasing the knowledge by evaluating a promising poxvirus-based vector vaccine that carries and expresses an artificial, computationally derived hemagglutini...

  4. Modelling and tests, at Fessenheim power station of a 1080 MvA turbogenerator and of its excitation system

    International Nuclear Information System (INIS)

    The studies and tests presented aim at a better knowledge of the behavior of large turbogenerators and of their excitation systems relating to small disturbances, on account of ever more severe steady state stability problems. The tests on the 900 MW turbogenerator at Fessenheim nuclear power station, were performed using methods of frequency analysis and statistic correlation, and their objects were: checking excitation system performance; determining transfer function x/sub d/(p), x/sub q/(p), f(p) of the generator, during small disturbances superimposed on no-load and on-load operating conditions. Tests are compared with predetermination from mathematical models of the generator and of its excitation system. 5 refs

  5. A viral vectored prime-boost immunization regime targeting the malaria Pfs25 antigen induces transmission-blocking activity.

    Directory of Open Access Journals (Sweden)

    Anna L Goodman

    Full Text Available The ookinete surface protein Pfs25 is a macrogamete-to-ookinete/ookinete stage antigen of Plasmodium falciparum, capable of exerting high-level anti-malarial transmission-blocking activity following immunization with recombinant protein-in-adjuvant formulations. Here, this antigen was expressed in recombinant chimpanzee adenovirus 63 (ChAd63, human adenovirus serotype 5 (AdHu5 and modified vaccinia virus Ankara (MVA viral vectored vaccines. Two immunizations were administered to mice in a heterologous prime-boost regime. Immunization of mice with AdHu5 Pfs25 at week 0 and MVA Pfs25 at week 10 (Ad-MVA Pfs25 resulted in high anti-Pfs25 IgG titers, consisting of predominantly isotypes IgG1 and IgG2a. A single priming immunization with ChAd63 Pfs25 was as effective as AdHu5 Pfs25 with respect to ELISA titers at 8 weeks post-immunization. Sera from Ad-MVA Pfs25 immunized mice inhibited the transmission of P. falciparum to the mosquito both ex vivo and in vivo. In a standard membrane-feeding assay using NF54 strain P. falciparum, oocyst intensity in Anopheles stephensi mosquitoes was significantly reduced in an IgG concentration-dependent manner when compared to control feeds (96% reduction of intensity, 78% reduction in prevalence at a 1 in 5 dilution of sera. In addition, an in vivo transmission-blocking effect was also demonstrated by direct feeding of immunized mice infected with Pfs25DR3, a chimeric P. berghei line expressing Pfs25 in place of endogenous Pbs25. In this assay the density of Pfs25DR3 oocysts was significantly reduced when mosquitoes were fed on vaccinated as compared to control mice (67% reduction of intensity, 28% reduction in prevalence and specific IgG titer correlated with efficacy. These data confirm the utility of the adenovirus-MVA vaccine platform for the induction of antibodies with transmission-blocking activity, and support the continued development of this alternative approach to transmission-blocking malaria subunit

  6. An update on TroVax® for the treatment of progressive castration-resistant prostate cancer

    Directory of Open Access Journals (Sweden)

    Abern M

    2011-05-01

    Full Text Available Michael Abern1, Howard L Kaufman2, Kalyan Latchamsetty11Department of Urology, Rush University Medical Center, Chicago, IL, USA; 2Department of General Surgery and Immunology and Microbiology, Rush University Medical Center, Chicago, IL, USAAbstract: Prostate cancer is a common human malignancy with few effective therapeutic options for treating advanced castration-resistant disease. The potential therapeutic effectiveness of immunotherapy and vaccines, in particular, has gained popularity based on the identification of prostate-associated antigens, potent expression vectors for vaccination, and data from recent clinical trials. A modified vaccinia Ankara (MVA virus expressing 5T4, a tumor-associated glycoprotein, has shown promise in preclinical studies and clinical trials in patients with colorectal and renal cell carcinoma. This review will discuss the rationale for immunotherapy in prostate cancer and describe preclinical and limited clinical data in prostate cancer for the MVA-5T4 (TroVax® vaccine.Keywords: castration resistance, prostate cancer, TroVax, vaccine

  7. Novel Conserved-region T-cell Mosaic Vaccine With High Global HIV-1 Coverage Is Recognized by Protective Responses in Untreated Infection.

    Science.gov (United States)

    Ondondo, Beatrice; Murakoshi, Hayato; Clutton, Genevieve; Abdul-Jawad, Sultan; Wee, Edmund G-T; Gatanaga, Hiroyuki; Oka, Shinichi; McMichael, Andrew J; Takiguchi, Masafumi; Korber, Bette; Hanke, Tomáš

    2016-04-01

    An effective human immunodeficiency virus type 1 (HIV-1) vaccine is the best solution for halting the acquired immune deficiency syndrome epidemic. Here, we describe the design and preclinical immunogenicity of T-cell vaccine expressing novel immunogens tHIVconsvX, vectored by DNA, simian (chimpanzee) adenovirus, and poxvirus modified vaccinia virus Ankara (MVA), a combination highly immunogenic in humans. The tHIVconsvX immunogens combine the three leading strategies for elicitation of effective CD8(+) T cells: use of regions of HIV-1 proteins functionally conserved across all M group viruses (to make HIV-1 escape costly on viral fitness), inclusion of bivalent complementary mosaic immunogens (to maximize global epitope matching and breadth of responses, and block common escape paths), and inclusion of epitopes known to be associated with low viral load in infected untreated people (to induce field-proven protective responses). tHIVconsvX was highly immunogenic in two strains of mice. Furthermore, the magnitude and breadth of CD8(+) T-cell responses to tHIVconsvX-derived peptides in treatment-naive HIV-1(+) patients significantly correlated with high CD4(+) T-cell count and low viral load. Overall, the tHIVconsvX design, combining the mosaic and conserved-region approaches, provides an indisputably better coverage of global HIV-1 variants than previous T-cell vaccines. These immunogens delivered in a highly immunogenic framework of adenovirus prime and MVA boost are ready for clinical development. PMID:26743582

  8. Characterization of T-cell responses to conserved regions of the HIV-1 proteome in BALB/c mice.

    Science.gov (United States)

    Ondondo, Beatrice; Abdul-Jawad, Sultan; Bridgeman, Anne; Hanke, Tomáš

    2014-11-01

    A likely requirement for a protective vaccine against human immunodeficiency virus type 1 (HIV-1)/AIDS is, in addition to eliciting antibody responses, induction of effective T cells. To tackle HIV-1 diversity by T-cell vaccines, we designed an immunogen, HIVconsv, derived from the most functionally conserved regions of the HIV-1 proteome and demonstrated its high immunogenicity in humans and rhesus macaques when delivered by regimens combining plasmid DNA, nonreplicating simian (chimpanzee) adenovirus ChAdV-63, and nonreplicating modified vaccinia virus Ankara (MVA) as vectors. Here, we aimed to increase the decision power for iterative improvements of this vaccine strategy in the BALB/c mouse model. First, we found that prolonging the period after the ChAdV63.HIVconsv prime up to 6 weeks increased the frequencies of HIV-1-specific, gamma interferon (IFN-γ)-producing T cells induced by the MVA.HIVconsv boost. Induction of strong responses allowed us to map comprehensively the H-2(d)-restricted T-cell responses to these regions and identified 8 HIVconsv peptides, of which three did not contain a previously described epitope and were therefore considered novel. Induced effector T cells were oligofunctional and lysed sensitized targets in vitro. Our study therefore provides additional tools for studying and optimizing vaccine regimens in this commonly used small animal model, which will in turn guide vaccine improvements in more expensive nonhuman primate and human clinical trials. PMID:25230940

  9. Vaccine efficacy against malaria by the combination of porcine parvovirus-like particles and vaccinia virus vectors expressing CS of Plasmodium.

    Directory of Open Access Journals (Sweden)

    Dolores Rodríguez

    Full Text Available With the aim to develop an efficient and cost-effective approach to control malaria, we have generated porcine parvovirus-like particles (PPV-VLPs carrying the CD8(+ T cell epitope (SYVPSAEQI of the circumsporozoite (CS protein from Plasmodium yoelii fused to the PPV VP2 capsid protein (PPV-PYCS, and tested in prime/boost protocols with poxvirus vectors for efficacy in a rodent malaria model. As a proof-of concept, we have characterized the anti-CS CD8(+ T cell response elicited by these hybrid PPV-VLPs in BALB/c mice after immunizations with the protein PPV-PYCS administered alone or in combination with recombinant vaccinia virus (VACV vectors from the Western Reserve (WR and modified virus Ankara (MVA strains expressing the entire P. yoelii CS protein. The results of different immunization protocols showed that the combination of PPV-PYCS prime/poxvirus boost was highly immunogenic, inducing specific CD8+ T cell responses to CS resulting in 95% reduction in liver stage parasites two days following sporozoite challenge. In contrast, neither the administration of PPV-PYCS alone nor the immunization with the vectors given in the order poxvirus/VLPs was as effective. The immune profile induced by VLPs/MVA boost was associated with polyfunctional and effector memory CD8+ T cell responses. These findings highlight the use of recombinant parvovirus PPV-PYCS particles as priming agents and poxvirus vectors, like MVA, as booster to enhance specific CD8+ T cell responses to Plasmodium antigens and to control infection. These observations are relevant in the design of T cell-inducing vaccines against malaria.

  10. Ankara Üniversitesi Tıp Fakültesi Hastaneleri ve Ankara Numune Hastanesinde çalışan doktor ve hemşirelerde tükenmişlik düzeyleri

    OpenAIRE

    Haran, Seda; Devrimci Özgüven, Halise; Ölmez, Şenay; Sayıl, IÅŸık

    1998-01-01

    Bu çalışmanın amacı A.Ü. Tıp Fakültesi\\\\'nde çalışandoktor ve hemşireler arasında tükenmişliksendromunun yaygınlığını saptamak ve tükenmişlikdüzeyini etkileyen faktörleri belirlemektir. Verilerİç Hastalıkları Servisi\\\\'nde çalışan 109 Hemşire ve56 doktordan, Maslach Tükenmişlik Ölçeği, İş DoyumuÖlçeği ve Çok Yönlü Sosyal Destek Ölçeğiuygulanarak toplanmıştır. Araştırma sonuçlarınagöre, hemşireler ve doktorlar arasında duygusal tükenmealtölçeğinde anlamlı fark bulunmuş, bunakar...

  11. Determination of Fatty Acid Composition on Different False Flax (Camelina sativa (L.) Crantz) Genotypes under Ankara Ecological Conditions

    OpenAIRE

    KATAR, Duran

    2013-01-01

    This research was conducted to determine fatty acid composition of different false flax (Camelina sativa (L.) Crantz) genotypes (Vinimik 17, PI 304269, CR 476/65, CR 1674/90, Ames26665, Ames26667, Ames26673, Ames26676, Ames26680, Ames26686 and Ames28372) in terms of oleic, linoleic, linolenic, steraic, eicosenoic and erucic acids in 2010 and 2011. Results showed that having highest linolenic acid content, Ames28372 could be suggested to be used as oil crops for oil industry or as medicine pla...

  12. Organochlorine pesticide residues in breast milk and maternal psychopathologies and infant growth from suburban area of Ankara, Turkey.

    Science.gov (United States)

    Yalçın, Songul Siddika; Örün, Emel; Yalçın, Suzan; Aykut, Osman

    2015-01-01

    We aimed to determine the levels of organochlorine pesticides (OCPs) in the breast milk; to assess the effect of detectable OCPs on maternal-infant characteristics and; to evaluate the relation between OCPs and the maternal psychopathologies [Brief Symptom Inventory (BSI), Postpartum Bonding Questionnaire (PBQ), Mother-to-Infant Bonding Scale (MIBS) and Edinburgh Postnatal Depression Scale (EPDS)] and infant growth. DDT, beta-hexachlorocyclohexane (HCH), aldrin and heptachlor were detected in 89.3, 70.7, 58.7 and 34.7 % of the samples, respectively. Mothers with low monthly family income had detectable DDTs less frequently. The frequencies of detectable heptachlor epoxide were significantly higher in mothers with gestational nausea. Anaemic mothers had more frequently detectable alpha-HCH. Z scores of head circumference were inversely correlated with beta-HCH and DDT levels. The heptachlor epoxide levels were positively correlated with PBQ, MIBS and indexes of BSI. No relation was detected between EPDS and OCPs. Further studies are needed for changes in maternal psychopathologies. PMID:25155352

  13. Structural Dimensions and Functions of Student Centers in the Open Education Faculty Practices: Three Metropolis Sampling - Istanbul, Ankara and Izmir

    OpenAIRE

    TUNÇKAN, Ergun

    2007-01-01

    The Open Education Faculty Students Centers have been offering many services to students in Turkey since 1982. Building up bridges between students and faculties; student’s centers have had technological improvements since 1998 and thereafter quality of services have been increased and services given to students at the student’s center have been variated by implementing a low-cost computer network. Operations of the student’s centers in The Open Education system, modifications taking place in...

  14. STRUCTURAL DIMENSIONS AND FUNCTIONS OF STUDENTS CENTERS IN THE OPEN UNIVERSITY PRACTICES: Three Metropolis Samplings - Istanbul, Ankara and Izmir

    OpenAIRE

    Ergun TUNCKAN

    2007-01-01

    The Open Education Faculty Students Centers have been offering many services to students in Turkey since 1982. Building up bridges between students and faculties; student’s centers have had technological improvements since 1998 and thereafter quality of services have been increased and services given to students at the student’s center have been variated by implementing a low-cost computer network. Operations of the student’s centers in The Open Education system, modifications taking place in...

  15. A Research on Expectation and Perception of Service Quality in Tourism Education at University Level in Ankara

    Directory of Open Access Journals (Sweden)

    Gonca Güzel Şahin

    2011-12-01

    Full Text Available To supply qualified manpower that is necessary in tourism sector, “quality of education” is one most important factor in higher education institutions. In this study, expectations on tourism education and level of satisfaction of the students that are the most important part of higher education institutions have been researched. In the research, service quality measurement models that are listed in the literature have been used to measure service quality in higher education institutions. By using the servqual model, the level of meeting the expectations of students about tourism education served by higher education institutions has been measured and important aspects of service quality according to students have been showed. The results of this study that is focused on tourism education and service quality show that the students, one of the major groups of higher education institutions, have a very low level of satisfaction about their education.

  16. EFL Teachers’ Perceptions of The Place of Culture in ELT: A Survey Study at Four Universities in Ankara/Turkey

    OpenAIRE

    Önalan, Okan

    2005-01-01

    This study aims to investigate Turkish teachers’ opinions and beliefs on the place of target cultural information in English language teaching, as well as their related practices and applications in EFL classrooms in Turkish higher education context. Particularly, it tries to explore three research questions: (a) How do Turkish teachers of English define culture? (b) What are the EFL teachers’ attitudes towards incorporating cultural information into their teaching? and (c) What role do they ...

  17. 78 FR 76821 - Executive-Led U.S.-Turkey Healthcare Trade Mission to Ankara, Istanbul, and Izmir

    Science.gov (United States)

    2013-12-19

    ... published at 78 FR 18318 (March 26, 2013), regarding the Executive-Led U.S.-Turkey Healthcare Trade Mission... to allow for additional recruitment and marketing in support of the mission. Applications will now be... evaluated in accordance with the Notice published at 78 FR 18318 (March 26, 2013) regarding the Amendment...

  18. The role of HbA1c as a screening and diagnostic test for diabetes mellitus in Ankara

    OpenAIRE

    GİNİŞ, Zeynep; Öztürk, Gülfer; SIRMALI, Rana; YALÇINDAĞ, Ali; DÜLGEROĞLU, Yakup; DELİBAŞI, Tuncay; DELİBAŞ, Namık

    2011-01-01

    This study investigated the value of HbA1c as a screening and diagnostic test for diabetes mellitus (DM) in high-risk Turkish individuals. Materials and methods: A total of 295 participants were successfully screened. Patients were divided into 4 groups based on their oral glucose tolerance test results, according to criteria put forth by the American Diabetes Association; 120 (40.7%) had normoglycemia, 44 (14.9%) had DM, 62 (21%) had impaired fasting glucose, and 69 (23.4%) had impaired gl...

  19. Perceptions of Students in Different Phases of Medical Education of Educational Environment: Ankara University Faculty of Medicine

    Directory of Open Access Journals (Sweden)

    M. Demirören,

    2008-01-01

    Full Text Available The present study was undertaken to identify the perceptions of students about their educational environment in a newly restructured curriculum. The Turkish version of the DREEM questionnaire (total score: 200 was used to diagnose the strengths and weaknesses of the curriculum which is known to be a major determinant of educational environment. Five hundred fifty three students (years 1, 3, 5 voluntarily replied to the questionnaire. The mean DREEM score was found to be 117.63 (58.8%. The mean scores for the whole DREEM questionnaire and the five essential domains were found to be significantly different in different phases of medical education. The scores were found to be highest (123.65 for year 3 students and lowest (109.39 for year 5 students. The results are the first data of a curriculum reform obtained from the students about the educational environment and give important feedback to curriculum planners and change managers of the faculty for necessary improvement.

  20. Perceptions of Students in Different Phases of Medical Education of Educational Environment: Ankara University Faculty of Medicine

    OpenAIRE

    Demirören, M.; Palaoglu, Ö.; Kemahli, S.; F.Özyurda; I.H. Ayhan

    2009-01-01

    The present study was undertaken to identify the perceptions of students about their educational environment in a newly restructured curriculum. The Turkish version of the DREEM questionnaire (total score: 200) was used to diagnose the strengths and weaknesses of the curriculum which is known to be a major determinant of educational environment. Five hundred fifty three students (years 1, 3, 5) voluntarily replied to the questionnaire. The mean DREEM score was found to be 117.63 (58.8%). The ...

  1. Teachers and Administrators' Views on the Implementation of Career Stages in the Teaching Profession (Case of Turkey--Ankara)

    Science.gov (United States)

    Gulcan, Murat Gurkan

    2010-01-01

    The criteria for teachers' climbing the career steps have been determined with the Law No. 5204, which was enacted in 2004 after long debates about making a career in the teaching profession. In the preamble of the law, it is stated that "teachers' professional and personal development should be ensured on the basis of their seniority, education,…

  2. Modelling and Analysing of Electricity Transmission Infrastructure of Ankara, Turkey: A Case Study on the Critical Line Scenarios

    OpenAIRE

    Ramazan BAYINDIR; YESILBUDAK Mehmet; Cetinkaya, Umut

    2015-01-01

    The use of electricity has been increasing constantly from past to now and there is a need of reliable transmission and distribution systems in order to provide continuous and balanced energy. Besides, traditional energy management systems have been forced to change as a result of increases in the usage of renewable energy resources and the efficiency of demand-side on the market. In this respect, power systems should be planned and operated, properly and the balance of generation-consumption...

  3. Recombinant modified vaccinia virus Ankara expressing glycoprotein E2 of Chikungunya virus protects AG129 mice against lethal challenge

    NARCIS (Netherlands)

    Doel, van den P.; Volz, A.; Roose, J.M.; Sewbalaksing, V.D.; Pijlman, G.P.; Middelkoop, van I.; Duiverman, V.; Wetering, van de E.; Sutter, G.; Osterhaus, A.D.; Martina, B.E.

    2014-01-01

    Chikungunya virus (CHIKV) infection is characterized by rash, acute high fever, chills, headache, nausea, photophobia, vomiting, and severe polyarthralgia. There is evidence that arthralgia can persist for years and result in long-term discomfort. Neurologic disease with fatal outcome has been docum

  4. Recombinant Modified Vaccinia Virus Ankara Expressing Glycoprotein E2 of Chikungunya Virus Protects AG129 Mice against Lethal Challenge

    NARCIS (Netherlands)

    P. van den Doel (Petra); A. Volz (Asisa); J.M. Roose (Jouke M.); V.D. Sewbalaksing (Varsha); G.P. Pijlman (Gorben); I. van Middelkoop (Ingeborg); V. Duiverman (Vincent); E. van de Wetering (Eva); G. Sutter (Gerd); A.D.M.E. Osterhaus (Albert); B.E.E. Martina (Byron)

    2014-01-01

    textabstractChikungunya virus (CHIKV) infection is characterized by rash, acute high fever, chills, headache, nausea, photophobia, vomiting, and severe polyarthralgia. There is evidence that arthralgia can persist for years and result in long-term discomfort. Neurologic disease with fatal outcome ha

  5. ADİL TİCARET VE ORGANİK GIDA PAZARLARI: ANKARA ÖRNEĞİ

    OpenAIRE

    ARSLANTÜRK, Yalçın; AYSEN, Esin

    2015-01-01

    Fair trade can be regarded as an output of sustainable development initiatives, promising social, economic as well as environmental improvements. This movement targets farmers and small-scale producers to get what they deserve in return for their labor and to play a greater role in the international trade. This study sets out to investigate the awareness of the tradesman in the organic food market, applications in Turkey in addition to the present situation in Turkey in terms of the instituti...

  6. Prediction of Water Quality Parameters Using Statistical Methods: A Case Study in a Specially Protected Area, Ankara, Turkey

    Science.gov (United States)

    Alp, E.; Yücel, Ö.; Özcan, Z.

    2014-12-01

    Turkey has been making many legal arrangements for sustainable water management during the harmonization process with the European Union. In order to make cost effective and efficient decisions, monitoring network in Turkey has been expanding. However, due to time and budget constraints, desired number of monitoring campaigns can not be carried. Hence, in this study, independent parameters that can be measured easily and quickly are used to estimate water quality parameters in Lake Mogan and Eymir using linear regression. Nonpoint sources are one of the major pollutant components in Eymir and Mogan lakes. In this paper, a correlation between easily measurable parameters, DO, temperature, electrical conductivity, pH, precipitation and dependent variables, TN, TP, COD, Chl-a, TSS, Total Coliform is investigated. Simple regression analysis is performed for each season in Eymir and Mogan lakes by using SPSS Statistical program using the water quality data collected between 2006-2012. Regression analysis demonstrated significant linear relationship between measured and simulated concentrations for TN (R2=0.86), TP (R2=0.85), TSS (R2=0.91), Chl-a (R2=0.94), COD (R2=0.99), T. Coliform (R2=0.97) which are the best results in each season for Eymir and Mogan Lakes. The overall results of this study shows that by using easily measurable parameters even in ungauged situation the water quality of lakes can be predicted. Moreover, the outputs obtained from the regression equations can be used as an input for water quality models such as phosphorus budget model which is used to calculate the required reduction in the external phosphorus load to Lake Mogan to meet the water quality standards.

  7. Vaccinia Virus LC16m8∆ as a Vaccine Vector for Clinical Applications

    Directory of Open Access Journals (Sweden)

    Minoru Kidokoro

    2014-10-01

    Full Text Available The LC16m8 strain of vaccinia virus, the active ingredient in the Japanese smallpox vaccine, was derived from the Lister/Elstree strain. LC16m8 is replication-competent and has been administered to over 100,000 infants and 3,000 adults with no serious adverse reactions. Despite this outstanding safety profile, the occurrence of spontaneously-generated large plaque-forming virulent LC16m8 revertants following passage in cell culture is a major drawback. We identified the gene responsible for the reversion and deleted the gene (B5R from LC16m8 to derive LC16m8Δ. LC16m8∆ is non-pathogenic in immunodeficient severe combined immunodeficiency (SCID mice, genetically-stable and does not reverse to a large-plaque phenotype upon passage in cell culture, even under conditions in which most LC16m8 populations are replaced by revertants. Moreover, LC16m8∆ is >500-fold more effective than the non-replicating vaccinia virus (VV, Modified Vaccinia Ankara (MVA, at inducing murine immune responses against pathogenic VV. LC16m8∆, which expresses the SIV gag gene, also induced anti-Gag CD8+ T-cells more efficiently than MVA and another non-replicating VV, Dairen I minute-pock variants (DIs. Moreover, LC16m8∆ expressing HIV-1 Env in combination with a Sendai virus vector induced the production of anti-Env antibodies and CD8+ T-cells. Thus, the safety and efficacy of LC16m8∆ mean that it represents an outstanding platform for the development of human vaccine vectors.

  8. Vaccination of colorectal cancer patients with TroVax given alongside chemotherapy (5-fluorouracil, leukovorin and irinotecan) is safe and induces potent immune responses.

    Science.gov (United States)

    Harrop, Richard; Drury, Noel; Shingler, William; Chikoti, Priscilla; Redchenko, Irina; Carroll, Miles W; Kingsman, Susan M; Naylor, Stuart; Griffiths, Richard; Steven, Neil; Hawkins, Robert E

    2008-07-01

    Modified vaccinia Ankara (MVA) encoding the tumor antigen 5T4 (TroVax) has been evaluated in an open label phase II study in metastatic colorectal cancer patients. The primary objective was to assess the safety and immunogenicity of TroVax injected before, during and after treatment with 5-fluorouracil, leukovorin and irinotecan. TroVax was administered to 19 patients with metastatic colorectal cancer. Twelve patients had blood samples taken following each of the six injections and were considered to be evaluable for assessment of immunological responses. Both antibody and cellular responses specific for the tumor antigen 5T4 and the viral vector MVA were monitored throughout the study. Administration of TroVax alongside chemotherapy was safe and well tolerated with no SAEs attributed to the vaccine and no enhancement of chemo-related toxicity. Of the 12 patients who were evaluable for assessment of immune responses, ten mounted 5T4-specific antibody responses with titers ranging from 10 to > 5,000. IFNgamma ELISPOT responses specific for 5T4 were detected in 11 patients with frequencies exceeding one in 1,000 PBMCs in five patients. Eight patients presented with elevated circulating CEA concentrations, six of whom showed decreases in excess of 50% during chemotherapy and four had CEA levels which remained stable for > 1 month following completion of chemotherapy. Of the 19 intention to treat (ITT) patients, one had a CR, six had PRs and five had SD. Potent 5T4-specific cellular and/or humoral immune responses were induced in all 12 evaluable patients and were detectable in most patients during the period in which chemotherapy was administered. These data demonstrate that TroVax can be layered on top of chemotherapy regimens without any evidence of enhanced toxicity or reduced immunological or therapeutic efficacy. PMID:18060404

  9. Estudio de la degradación de elementos refractarios de alúmina en la sinterización de aceros modificados con Mn

    OpenAIRE

    Peña, P.; Sicre-Artalejo, J.; Campos, M; Torralba Castelló, José Manuel; Zbiral, J.; Danniger, H.

    2008-01-01

    [ES] En este trabajo se estudia la degradación de los refractarios de un horno de sinterización, cuando están involucrados en el proceso aceros de baja aleación con adición de manganeso. Dada la elevada Pvapor del manganeso y las atmósferas habituales de sinterización para este tipo de aceros, se produce su sublimación durante el ciclo térmico. Se proporciona así, una fase gaseosa, que pudiendo ser beneficiosa para la difusión en las muestras de acero, puede alterar la composición de los refr...

  10. O desenvolvimento biotecnológico e as suas implicações jurídicas na utilização de organismos geneticamente modificados

    OpenAIRE

    Silva, Kamila Regina da

    2013-01-01

    A reflexão proporcionada pela bioética é absolutamente necessária diante do atual progresso científico, sendo imprescindível para o estudo das implicações geradas pela biotecnologia a análise de sua origem histórica, princípios e conceitos. O precursor nos estudos envolvendo preceitos da biotecnologia foi o austríaco Johann Gregor Mendel, a partir de suas constatações foi possível aduzir o conceito de "genes". No entanto, apenas em 1953, quando os médicos James Watson e Francis Crick descobri...

  11. Caracterización estructural y electroquímica de materiales Sonogel-Carbono modificados y su empleo como sensores electroquímicos selectivos

    OpenAIRE

    2007-01-01

    En esta Tesis Doctoral se ha abordado la caracterización estructural y electroquímica de los nuevos materiales Sonogel-Carbono, desarrollados y patentados por nuestro grupo de investigación, y de sus modificaciones. El planteamiento y la finidad del trabajo se han dirigido hacie el conocimiento de la estructura y de la superficie de los materiales, con el fin de poder comprender la utilidad y el comportamiento de lo sensores electroquímicos desarrollados. Para ello se han empleado diversas té...

  12. Estudo do comportamento térmico e mecânico do PLA modificado com aditivo nucleante e modificador de impacto

    Directory of Open Access Journals (Sweden)

    Renato Brisigueli Pereira

    2014-01-01

    Full Text Available O poliácido lático (PLA é, dentre os polímeros biodegradáveis, o que apresenta o maior potencial para substituição aos polímeros derivados do petróleo na indústria de embalagens. No entanto uma de suas principais limitações está na baixa estabilidade térmica dimensional. O presente trabalho apresenta um estudo da influência da utilização de dois aditivos: modificador térmico (nucleante e modificador de impacto, na Temperatura de Distorção Térmica (HDT, no Ponto de Amolecimento Vicat (Vicat e nas propriedades mecânicas do PLA. Os mecanismos de atuação destes aditivos foram estudados por alterações na temperatura de transição vítrea (Tg e na cristalinidade medidas por calorimetria diferencial exploratória (DSC em corpos de prova injetados sem e com tratamento de recozimento. Aplicou-se um planejamento experimental para tratamento estatístico dos dados. Os valores de HDT e de Vicat foram pouco alterados pela presença dos aditivos, embora tenha sido observado aumento nos valores do módulo elástico de tração. O tratamento de recozimento foi responsável pelo aumento tanto na HDT como na Vicat devido ao aumento no grau de cristalinidade. As curvas de DSC mostraram uma grande complexidade no comportamento térmico do PLA, sendo fortemente influenciado pelas condições de tratamento térmico.

  13. Oxidación de cianuro en disolución acuosa mediante ozonización en presencia de carbón activado modificado

    Directory of Open Access Journals (Sweden)

    M. A. Sánchez Castillo

    2013-01-01

    Full Text Available El objetivo de este trabajo fue analizar el papel quedesempeña el carbón activado en el proceso deozonización del anión cianuro. Los resultadosobtenidos muestran que el carbón activadopreviamente tratado con amoniaco incrementóconsiderablemente la velocidad de eliminación delanión cianuro. Los resultados obtenidos medianteFTIR y XPS muestran un incremento en laconcentración de grupos carbonilo, los cuales conla interacción con el ozono podrían potenciar lageneración de radicales altamente oxidantes en elmedio justificándose así los resultados obtenidos.

  14. Eletrodos quimicamente modificados aplicados à eletroanálise: uma breve abordagem Chemically modified electrodes applyes to electroanalysis: a brief presentation

    Directory of Open Access Journals (Sweden)

    Maria de Fátima Brito Souza

    1997-04-01

    Full Text Available Chemically modified electrodes (CMEs have been subject of considerable attention since its inception about 23 years ago. CMEs result of a deliberate immobilization of a modifier agent onto the electrode surface obtained through chemical reactions, chemisorption, composite formation or polymer coating. This immobilization seeks transfer the physicochemical properties of the modifier to the electrode surface and thus to dictate and control the behavior of the electrode/solution interface. In recent years the interest in CMEs has increased particularly to enhance the sensitivity and/or the selectivity of electroanalytical techniques. In general higher sensitivity and/or selectivity may be achieved by exploiting one or more of the following phenomena: electrocatalysis, preconcentration and interferents exclusion. This paper deals with the application of CMEs in electroanalysis, including a brief presentation of the more general procedures that have been employed for the modification of electrode surfaces.

  15. Alimentos modificados e suas implicações no metabolismo energético Effects of modified foods on energy metabolism

    OpenAIRE

    Denise Machado Mourão; Josefina Bressan Resende Monteiro; Helen Hermana Miranda Hermsdorff; Marta Cristina Leite Teixeira

    2005-01-01

    OBJETIVO: Comparar o efeito de duas dietas, modificadas no perfil de seus carboidratos, no metabolismo energético de homens com peso normal e com sobrepeso. MÉTODOS: Três formulações convencionais e suas formulações correspondentes diet, compuseram as dietas isoenergéticas padrão e a dieta modificada, respectivamente. Utilizou-se a calorimetria indireta, para obtenção dos dados referentes ao metabolismo energético. A amostra foi de 13 homens em cada grupo. RESULTADOS: O grupo com sobrepeso ap...

  16. Study of gel formation in polypropylene modified by gamma irradiation; Estudo da formacao de geis em polipropileno modificado por irradiacao gama

    Energy Technology Data Exchange (ETDEWEB)

    Oliani, Washington Luiz; Parra, Duclerc Fernandes; Lima, Luis Filipe Carvalho Pedroso de; Lugao, Ademar Benevolo, E-mail: washoliani@usp.br [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil); Riella, Humberto Gracher [Universidade Federal de Santa Catarina (UFSC), Florianopolis, SC (Brazil)

    2010-07-01

    The linearity of the chains of iPP (isotactic polypropylene) confers to this, low melt strength. This fact limits the use of iPP in processes that demand high stretching. The graft of branches confers improvements in its extensional viscosity, resulting in Polypropylene with High Melt Strength (HMS-PP). Preparation process of the HMS-PP, included iPP in pellets, conditioned in plastic container containing acetylene under pressure of 110 kPa and radiation with γ source of {sup 60}Co in the doses of 5, 12.5 and 20 kGy. The gel fraction of the samples was determined by the extraction of soluble components in xylene under boiling for 12 hours at 138 °C. The soluble part of the samples was decanted with the total volatilization of the xilene to the room temperature (25 °C) and deposition in glass blades. These samples had been characterized by: Optic Microscopy (MO), Scanning Electron Microscopy (SEM) and infrared Spectroscopy (FT-IR). In this study of the morphology, we obtained the formation of gel and microgel of polypropylene with higher incidence in HMS-PP 20 kGy. (author)

  17. Validez incremental del Test Gestáltico de Bender Modificado, en niños que inician el primer grado

    Directory of Open Access Journals (Sweden)

    César A. Merino Soto

    2014-04-01

    Full Text Available The Bender Gestalt Test (BGT has a long history in psychological assessment and several scoring systems have been developed for it. One of them is the Brannigan and Brunner’s Qualitative Scoring System (QSS, developed for the modified version of this test that uses six of the nine original designs (BGT Modified. However, there is limited psychometric evidence for the Spanish speaking population about the usefulness of this measure in an academic screening battery. The purpose of this study is to report evidence of incremental validity of this abbreviated version regarding school achievement, within a group of 101 five year olds entering the first grade of primary education. We applied a hierarchical multiple regression controlling for the effects of fluid intelligence and pre-academic skills, compared to the criteria of school grades. We found that the inclusion of BGT Modified brings significant unique variance in school grades, and is a more powerful predictor than intelligence. Our results support the use of BGT Modified in a brief battery of screening in identifying children at risk for poor school performance

  18. Impacto Ambiental y Económico de la Liberalización de Maíz Genéticamente Modificado (GM) en Chile

    OpenAIRE

    Osorio, José Díaz; Jara-Rojas, Roberto; Moya, Francisco

    2012-01-01

    J. Díaz Osorio, R. Jara-Rojas and F. Moya. 2012. Environmental and economic impact to the liberalization of genetic modified maize in Chile. Currently, about 125 million of hectares of GM crops are grown in 25 countries. Some authors establish that GM crops have contributed to reduce the environmental impact of agriculture and reduce the use of machinery and chemicals. In Chile, seed production of GM crops is only allowed for export purposes; however, it cannot be used as input for commercial...

  19. Efecto de una dieta con productos modificados de textura en pacientes ancianos ambulatorios Effects of a diet with products in texture modified diets in elderly ambulatory patients

    Directory of Open Access Journals (Sweden)

    D. A. De Luis

    2009-02-01

    Full Text Available Fundamentos: Las dificultades para deglutir alimentos y bebidas son un problema en la vida de muchas personas, constituyendo un claro detrimento en su calidad de vida, sobre todo en pacientes ancianos. El objetivo de nuestro trabajo es evaluar en pacientes ancianos ambulatorios con disfagia la influencia en variables nutricionales bioquímicas y antropométricas, así como en la calidad de vida de una dieta comercial modificada de textura lista para su uso incluida en la dieta oral de estos pacientes. Métodos: Estudiamos un total de 29 pacientes en la Consultade Nutrición con disfagia y una edad superior a 70años. Los pacientes recibieron una dieta modificada de textura y suplementada con un producto listo para su uso(Resource Ready® (1.633 kcal, 59,9 g proteínas (16%, 52,1g Lípidos (29%, 231,7 g Hidratos de carbono (57%, 11,1gde Fibra, 82 mg Colesterol. Antes de iniciar la dieta y a los 3 meses de seguimiento a los pacientes se les realizó una analítica, una valoración antropométrica (pliegue tricipital, circunferencia muscular del brazo, circunferencia del brazo, peso, talla, índice de masa corporal, impedanciometría tetrapolar, una encuesta nutricional de 3 días, así como el cuestionario de calidad de vida SF36. Resultados: Se reclutaron un total de 29 pacientes con una distribución por sexo de (18 mujeres y 11 varones, con una edad media de 79,5 ± 8,59 años. Un total de 25,7% presentaban un tumor de vía digestiva alta, un 24% demencia y un 41,3% otras alteraciones neurológicas. Se detectó una mejoría significativa en el peso, masa magra y masa magra de los pacientes tratados, con un aumento significativo de los niveles de proteínas totales, transferrína, albúmina y linfocitos. Las ingestas calórica, proteica, grasa y de hidratos de carbono aumentaron significativamente en términos absolutos. La ingesta calórica corregida por kg de peso mejoró significativamente (26,1 ± 5,7 cal/kg vs 28,9 ± 4,5 cal/kg: p Background: Decrease in the capacity to deglution solids and liquids is a problem in many people, this problem decreases quality of life. The main objective of our work was to evaluate the influence in elderly ambulatory patients with dysphagia of a texture modified diet ready to use on nutritional parameters and quality of life. Methods: We enrolled 29 patients from Nutrition Departament with dysphagia and an age over 70 years. Patients received a texture modified diet supplemented with dehydrated nutritional products (Resource Ready® (1,633 kcal, 59,9 g proteins (16%, 52,1 g lipids (29%, 231,7 g carbohydrates (57%, 11,1g de fiber, 82 mg cholesterol. Before and after 3 months of starting the treatment, we recorded a blood analysis, nutritional anthropometric evaluation (tricipital skinfold, midarm circumference, midarm muscle circumference, weight, height and body mass index, a bipolar bioimpedance and a dietary questionnaire of 3 days. SF36 as a questionnaire of quality of life was used before and after nutritional treatment. Results: A total of 29 patients were recluted with a sex distribution of (18 females and 11 males, and an average age of 79,5+8,59 years. A 25,7% of patients had head and neck tumors, a 24% dementia and a 41,3% chronic neurological deterioration. After treatment, we detected an improvement in weight, fat mass, fat free mass, total proteins, albumin, transferrin and lymphocytes. Caloric, protein fat and carbohydrate intakes increased in a significative way. Caloric intake corrected by weight improved (26.1 ± 5.7 cal/kg vs 28.9 ± 4.5 cal/kg: p < 0.05. Quality of life improved significantly (SF 36 score 94.7 ± 15 points vs 99.3 ± 10 points; p < 0.05. Conclusion: A texture modified diet with dehydrated nutritional products improved dietary intake, nutritional status and quality of life of ambulatory elderly patients with dysphagia.

  20. Synthesis of lithium silicates by the modified method of combustion. XRD and IR; Sintesis de silicatos de litio por el metodo modificado de combustion. DRX e IR

    Energy Technology Data Exchange (ETDEWEB)

    Cruz, D.; Bulbulian, S. [Instituto nacional de Investigaciones Nucleares, A.P. 18-1027, 11801 Mexico D.F. (Mexico)

    2002-07-01

    The combustion method is fixed in exothermic reactions for producing ceramic compounds. The precursor solutions are mixtures of metal nitrates and the fuels. This method was modified using non-oxidant compounds as lithium hydroxide and silicide acid and urea as fuel. The precursors were heated during 5 minutes at temperatures between 250 C and 550 C allowing so the mixture combustion. The obtained ceramics were characterized by X-ray diffraction and IR spectroscopy. The sample pollution with carbonates was evaluated and it was found that the presence of these diminish according as increase the calcination temperature. (Author)