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Sample records for andino boliviano puma

  1. Caracterización genética del puma andino boliviano (Puma concolor en el Parque Nacional Sajama (PNS y relaciones con otras poblaciones de pumas del noroccidente de Sudamérica Genetic characterization of the Bolivian Andean puma (Puma concolor at the Sajama National Park (SNP and relationships with other north-western South American puma populations

    Directory of Open Access Journals (Sweden)

    MANUEL RUIZ-GARCÍA

    2009-03-01

    Full Text Available Se analizaron originalmente 25 muestras fecales de puma andino boliviano para proceder a su extracción de ADN. De esas 25 muestras, se detectaron cinco pumas diferentes que, junto, a tres de pieles de animales cazados, completaron un total de ocho pumas andinos bolivianos analizados. Igualmente se analizaron 45 muestras de ADN procedentes de pumas silvestres de Colombia, Perú, Ecuador, Venezuela y Amazonia occidental brasileña obtenidas a partir de mechones de pelos con bulbo, trocitos de pieles, músculo y dientes. Todas ellas se genotipificaron para siete marcadores microsatélites (Fea 08, 24, 43, 45, 96, 126 y 391. Los niveles de diversidad genética resultaron muy elevados en ambas muestras (H = 0,942 y 0,845; respectivamente, con valores muy superiores a los reportados para pumas norteamericanos. Diversos análisis de asignación poblacional mostraron que los pumas andinos bolivianos no formaron un grupo consistentemente diferente del otro grupo de pumas analizado. Únicamente un marcador, Fea 96, mostró heterogeneidad genética significativa entre ambos grupos. Sin embargo, globalmente, esa heterogeneidad fue extremadamente pequeña (F ST, G ST, R ST. Por el contrario, las estimas de flujo génico entre ambas agrupaciones fueron elevadas para toaos los procedimientos empleados. La estimación del parámetro θ (= 4Neμ mediante el método de máxima verosimilitud de Nielsen (1997 mostró que la muestra boliviana es una extensión indiferenciable de la otra agrupación de pumas de otros países latinoamericanos. Por lo tanto, este estudio aporta resultados concluyentes en favor de un único acervo genético de pumas en el nor-occidente de Sudamérica, en contraste con las tradicionales clasificaciones morfológicas y morfométricas que habían identificado un número considerable de subespecies de puma en esta región de Latinoamérica.Twenty-five Andean Bolivian fecal samples were obtained for extracting DNA. Five different Andean

  2. Migración en el Sur boliviano : Contrastes entre valles andinos tradicionales y áreas orientales de colonización

    OpenAIRE

    Martin, Ceydric

    2012-01-01

    International audience; La relevancia de los resultados presentados en este volumen se debe a dos novedosos enfoques geográficos elegidos y profundizados por Ceydric Martin: el primero , abarca varias aldeas de los valles andinos cercanos a la ciudad de Tarija, y el segundo los límites del Chaco tarijeño, en donde la historia de migración -que en este caso es la inmigración- es reciente. Estos dos estudios tienen que leerse, y entenderse, en el marco de la larga historia de emigración que car...

  3. Puma (Puma concolor) epididymal sperm morphometry

    OpenAIRE

    Hernán Cucho; Virgilio Alarcón; César Ordóñez; Enrique Ampuero; Aydee Meza; Carles Soler

    2016-01-01

    The Andean puma (Puma concolor) has not been widely studied, particularly in reference to its semen characteristics. The aim of the present study was to define the morphometry of puma sperm heads and classify their subpopulations by cluster analysis. Samples were recovered postmortem from two epididymides from one animal and prepared for morphological observation after staining with the Hemacolor kit. Morphometric data were obtained from 581 spermatozoa using a CASA-Morph system, rendering 13...

  4. História, arte e política: o muralismo do boliviano Miguel Alandia Pantoja

    OpenAIRE

    Andrade,Everaldo de Oliveira

    2006-01-01

    Este artigo pretende avaliar em que medida arte e política se expressaram na criação e na ação do artista e militante político boliviano Miguel Alandia Pantoja, ativo participante de momentos centrais da História do seu país como a guerra do Chaco, a revolução de 1952 e a Comuna de La Paz de 1971. As influências do indigenismo andino e do muralismo mexicano parecem ter-se cruzado com as opções políticas de Alandia e com seu engajamento na proposta dos artistas ligados ao Manifesto por Uma Art...

  5. Puma (Puma concolor) epididymal sperm morphometry

    Science.gov (United States)

    Cucho, Hernán; Alarcón, Virgilio; Ordóñez, César; Ampuero, Enrique; Meza, Aydee; Soler, Carles

    2016-01-01

    The Andean puma (Puma concolor) has not been widely studied, particularly in reference to its semen characteristics. The aim of the present study was to define the morphometry of puma sperm heads and classify their subpopulations by cluster analysis. Samples were recovered postmortem from two epididymides from one animal and prepared for morphological observation after staining with the Hemacolor kit. Morphometric data were obtained from 581 spermatozoa using a CASA-Morph system, rendering 13 morphometric parameters. The principal component (PC) analysis was performed followed by cluster analysis for the establishment of subpopulations. Two PC components were obtained, the first related to size and the second to shape. Three subpopulations were observed, corresponding to elongated and intermediate-size sperm heads and acrosomes, to large heads with large acrosomes, and to small heads with short acrosomes. In conclusion, puma spermatozoa showed no uniform sperm morphology but three clear subpopulations. These results should be used for future work in the establishment of an adequate germplasm bank of this species. PMID:27678466

  6. Puma (Puma concolor epididymal sperm morphometry

    Directory of Open Access Journals (Sweden)

    Hernán Cucho

    2016-01-01

    Full Text Available The Andean puma (Puma concolor has not been widely studied, particularly in reference to its semen characteristics. The aim of the present study was to define the morphometry of puma sperm heads and classify their subpopulations by cluster analysis. Samples were recovered postmortem from two epididymides from one animal and prepared for morphological observation after staining with the Hemacolor kit. Morphometric data were obtained from 581 spermatozoa using a CASA-Morph system, rendering 13 morphometric parameters. The principal component (PC analysis was performed followed by cluster analysis for the establishment of subpopulations. Two PC components were obtained, the first related to size and the second to shape. Three subpopulations were observed, corresponding to elongated and intermediate-size sperm heads and acrosomes, to large heads with large acrosomes, and to small heads with short acrosomes. In conclusion, puma spermatozoa showed no uniform sperm morphology but three clear subpopulations. These results should be used for future work in the establishment of an adequate germplasm bank of this species.

  7. Puma (Puma concolor) epididymal sperm morphometry.

    Science.gov (United States)

    Cucho, Hernán; Alarcón, Virgilio; Ordóñez, César; Ampuero, Enrique; Meza, Aydee; Soler, Carles

    2016-01-01

    The Andean puma (Puma concolor) has not been widely studied, particularly in reference to its semen characteristics. The aim of the present study was to define the morphometry of puma sperm heads and classify their subpopulations by cluster analysis. Samples were recovered postmortem from two epididymides from one animal and prepared for morphological observation after staining with the Hemacolor kit. Morphometric data were obtained from 581 spermatozoa using a CASA-Morph system, rendering 13 morphometric parameters. The principal component (PC) analysis was performed followed by cluster analysis for the establishment of subpopulations. Two PC components were obtained, the first related to size and the second to shape. Three subpopulations were observed, corresponding to elongated and intermediate-size sperm heads and acrosomes, to large heads with large acrosomes, and to small heads with short acrosomes. In conclusion, puma spermatozoa showed no uniform sperm morphology but three clear subpopulations. These results should be used for future work in the establishment of an adequate germplasm bank of this species.

  8. The Perturbed Puma Model

    Science.gov (United States)

    Rong, Shu-Jun; Liu, Qiu-Yu

    2012-04-01

    The puma model on the basis of the Lorentz and CPT violation may bring an economical interpretation to the conventional neutrinos oscillation and part of the anomalous oscillations. We study the effect of the perturbation to the puma model. In the case of the first-order perturbation which keeps the (23) interchange symmetry, the mixing matrix element Ue3 is always zero. The nonzero mixing matrix element Ue3 is obtained in the second-order perturbation that breaks the (23) interchange symmetry.

  9. El Derecho Administrativo del Sistema Comunitario Andino

    OpenAIRE

    Jorge Enrique Santos Rodríguez

    2013-01-01

    Una de las tendencias contemporáneas del Derecho Administrativo es el reconocimiento de su existencia más allá de las fronteras de un Estado. Bajo esa premisa, se busca demostrar que en la Comunidad Andina de Naciones se configuran los elementos suficientes para afirmar que existe un Derecho Administrativo Comunitario Andino, en la medida en que de las normas andinas se desprende la existencia de una función administrativa andina, una organización administrativa al seno del Sistema Andino de ...

  10. El Derecho Administrativo del Sistema Comunitario Andino

    Directory of Open Access Journals (Sweden)

    Jorge Enrique Santos Rodríguez

    2013-12-01

    Full Text Available Una de las tendencias contemporáneas del Derecho Administrativo es el reconocimiento de su existencia más allá de las fronteras de un Estado. Bajo esa premisa, se busca demostrar que en la Comunidad Andina de Naciones se configuran los elementos suficientes para afirmar que existe un Derecho Administrativo Comunitario Andino, en la medida en que de las normas andinas se desprende la existencia de una función administrativa andina, una organización administrativa al seno del Sistema Andino de Integración, un sistema de fuentes administrativas andina y un contencioso administrativo comunitario.

  11. The Perturbed Puma Model

    Institute of Scientific and Technical Information of China (English)

    RONG Shu-Jun; LIU Qiu-Yu

    2012-01-01

    The puma model on the basis of the Lorentz and CPT violation may bring an economical interpretation to the conventional neutrinos oscillation and part of the anomalous oscillations.We study the effect of the perturbation to the puma model.In the case of the first-order perturbation which keeps the (23) interchange symmetry,the mixing matrix element Ue3 is always zero.The nonzero mixing matrix element Ue3 is obtained in the second-order perturbation that breaks the (23) interchange symmetry.%The puma model on the basis of the Lorentz and CPT violation may bring an economical interpretation to the conventional neutrinos oscillation and part of the anomalous oscillations. We study the effect of the perturbation to the puma model. In the case of the first-order perturbation which keeps the (23) interchange symmetry, the mixing matrix element Ue3 is always zero. The nonzero mixing matrix element Ue3 is obtained in the second-order perturbation that breaks the (23) interchange symmetry.

  12. História, arte e política: o muralismo do boliviano Miguel Alandia Pantoja History, art and politics: the muralism of the Bolivian Miguel Alandia Pantoja

    OpenAIRE

    Everaldo Oliveira Andrade

    2006-01-01

    Este artigo pretende avaliar em que medida arte e política se expressaram na criação e na ação do artista e militante político boliviano Miguel Alandia Pantoja, ativo participante de momentos centrais da História do seu país como a guerra do Chaco, a revolução de 1952 e a Comuna de La Paz de 1971. As influências do indigenismo andino e do muralismo mexicano parecem ter-se cruzado com as opções políticas de Alandia e com seu engajamento na proposta dos artistas ligados ao Manifesto por Uma Art...

  13. Biodigestores de bajo coste para climas andinos

    OpenAIRE

    Poggio, Davide; Paiva Prado, Margot; Ferrer Martí, Ivet; Velo García, Enrique

    2009-01-01

    En el Altiplano Andino existe una alta demanda de biodigestores anaeróbicos por parte de los pequeños productores ganaderos, a causa del escaso acceso a combustibles limpios y a fertilizantes eficientes, y a la abundancia de residuos agropecuarios. La difusión de biodigestores rurales en zonas montañosas o de clima frío ha sido tradicionalmente escasa, sobre todo a causa de la relación desfavorable entre las bajas temperaturas y la velocidad de la digestión. Durante el periodo 2007-2009 se ha...

  14. Ciclos peruanos, andinos y de Estados Unidos

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    Javier Iguíñiz Echeverría

    1997-12-01

    Full Text Available En este artículo hemos comparado las fluctuaciones de las economíasandinas entre sí durante 1950-1995 y la evolución cíclica de la economíaperuana y de Estados Unidos de Norteamérica entre 1929 y 1995. El resultadoprincipal de la comparación basada en la descomposición de las series por elmétodo determinístico y de Hodrick-Prescott, es que hasta 1981 los ciclosandinos están positiva y significativamente correlacionados entre sí con laexcepción de Venezuela. La sincronía con el ciclo de EE.UU. es mayor en elcaso de los países no petroleros del área, pero, en general, es menos robustaque la encontrada entre los países andinos.La correlación positiva y significativa entre países andinos y con EE.UU.desaparece en el período 1981-1995. La crisis de la deuda “desconecta” lastrayectorias de corto plazo. La correlación entre los ciclos de Perú y EE.UU.es positiva y significativa cuando se compara el período 1929-1981 y pierdesignificancia en el período 1981-1995.

  15. Erotismo, sexualidad y humor en las danzas del altiplano boliviano

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    Eveline Sigl

    2012-09-01

    Full Text Available El presente artículo analiza la importancia del erotismo, la sexualidad y el humor en las danzas del altiplano boliviano. Demuestra que estos elementos performativos no solo están fuertemente interconectados, sino también íntimamente ligados a la sacralidad andina. La primera parte del texto está dedicada a los componentes humorísticos y lúdicos del enamoramiento mediante la danza, postulando que humor y alegría forman parte del “erotismo andino”. Mientras tanto, la segunda parte estudia el “transformismo ritual andino”, que consiste en presentaciones dancísticas sumamente jocosas y fuertemente sexuadas a cargo de hombres hetero y homosexuales disfrazados de mujer.

  16. ABL Tyrosine Kinase Stimulates PUMA Protein Expression

    OpenAIRE

    Oon, Chet K

    2016-01-01

    ABL is an ubiquitously expressed non-receptor tyrosine kinase involved in multiple cellular functions including programmed cell death. Upon DNA damage, ABL has been shown to upregulate PUMA, p53 upregulated modulator of apoptosis, and causes downstream mitochondrial intrinsic apoptotic events. However, the mechanism by which ABL regulates PUMA expression remains unknown. We have shown that ABL does not change PUMA protein subcellular localization through immunofluorescence. Through protein an...

  17. PUMA test program for SBWR

    Energy Technology Data Exchange (ETDEWEB)

    Ishii, M.; Revankar, S.T.; Dowlati, R. [Purdue Univ., West Lafayette, IN (United States)] [and others

    1996-03-01

    The objective of the PUMA integral test program is to obtain confirmatory test data for the SBWR Developed by the General Electric-Nuclear Energy Company. The program was initiated in July 1993 under the sponsorship of the NRC. The SBWR has a simplified coolant circulation system and a passive emergency cooling system. The engineered safety systems and safety-grade systems in the SBWR are: (1) the Automatic Depressurization System (ADS), (2) the Gravity-Driven Cooling System (GDCS), (3) the Passive Containment Cooling System (PCCS), (4) the Isolation Condenser Systems (ICS), and (5) the Pressure Suppression Pool (SP). The GDCS and PCCS are new designs unique to the SBWR and do not exist in operating BWRs. The ICS is similar to those in some operating BWRs. The PCCS is designed for low-pressure operation for the containment cooling, but the ICS is capable of high pressure operation as well to cool the reactor pressure vessel. The PUMA design was completed based on an extensive scaling analysis. The PUMA facility having 1/4 height and 1/400 volume scales is constructed. Various facility characterization tests and instrumentation and data acquisition system checks are performed presently. The facility acceptance test will be performed in November and integral tests will be initiated.

  18. El legado de Pablo Dermizaky y su aporte al constitucionalismo boliviano (Homenaje póstumo

    Directory of Open Access Journals (Sweden)

    Alan E. Vargas Lima

    2015-01-01

    Full Text Available El presente estudio bio-bibliográfico, pretende realizar un bosquejo generalacerca de la vida, obra y pensamiento del eminente constitucionalista bolivianoDr. Pablo Dermizaky Peredo (†, describiendo algunos de sus rasgos biográficosmás importantes, y desarrollando a su vez, una revisión bibliográfica desus principales obras, con la finalidad de destacar: su loable labor académicade enseñanza del Derecho Constitucional, su indispensable contribución en laconsolidación del primer Tribunal Constitucional en Bolivia, y su extraordinarioaporte al desarrollo del constitucionalismo boliviano.

  19. PUMA: The Positional Update and Matching Algorithm

    CERN Document Server

    Line, J L B; Pindor, B; Mitchell, D A; Trott, C M

    2016-01-01

    We present new software to cross-match low-frequency radio catalogues: the Positional Update and Matching Algorithm (PUMA). PUMA combines a positional Bayesian probabilistic approach with spectral matching criteria, allowing for confusing sources in the matching process. We go on to create a radio sky model using PUMA based on the Murchison Widefield Array Commissioning Survey, and are able to automatically cross-match 98.5% of sources. Using the characteristics of this sky model, we create simple simulated mock catalogues on which to test PUMA, and find that PUMA can reliably find the correct spectral indices of sources, along with being able to recover ionospheric offsets. Finally, we use this sky model to calibrate and remove foreground sources from simulated interferometric data, generated using OSKAR (the Oxford University visibility generator). We demonstrate that there is a substantial improvement in foreground source removal when using higher frequency and higher resolution source positions, even when...

  20. Mitochondrial DNA sequence variation and phylogeography of Neotropic pumas (Puma concolor).

    Science.gov (United States)

    Caragiulo, Anthony; Dias-Freedman, Isabela; Clark, J Alan; Rabinowitz, Salisa; Amato, George

    2014-08-01

    Pumas occupy the largest latitudinal range of any New World terrestrial mammal. Human population growth and associated habitat reduction has reduced their North American range by nearly two-thirds, but the impact of human expansion in Central and South America on puma populations is not clear. We examined mitochondrial DNA diversity of pumas across the majority of their range, with a focus on Central and South America. Four mitochondrial gene regions (1140 base pairs) revealed 16 unique haplotypes differentiating pumas into three geographic groupings: North America, Central America and South America. These groups were highly differentiated as indicated by significant pairwise FST values. North American samples were genetically homogenous compared to Central and South American samples, and South American pumas were the most diverse and ancestral. These findings support an earlier hypothesis that North America was recolonized by founding pumas from Central and South America.

  1. EL español andino. II parte

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    Rubén Arboleda Toro

    2002-01-01

    Full Text Available En el número 13 de esta revista (nov. del 2000 se publicó una primera parte del estudio sobre el español andino. Presentamos ahora una segunda parte que comprende aspectos histórico-geográficos de Nariño y Putumayo andinos, región de Colombia donde se habla esa variedad, y una descripción general de su realidad lingüística. Esperamos que sean objeto de otra publicación la descripción de los rasgos dialectales del español andino, parte nuclear del trabajo, y la presentación de la metodología y el corpus. En esto nos encontramos trabajando. Incluimos no obstante un inventario de rasgos más amplio que el presentado en la primera parte. Pero por ahora se trata de eso, de un inventario ilustrativo, no del análisis en el que estamos empeñados, en el marco del contacto de lenguas, el cambio lingüístico y la relación entre la norma y las posibilidades del sistema. Para contextualizar esta segunda parte, incluimos, a manera de introducción, un resumen de la primera.

  2. Survival and Mortality of Pumas (Puma concolor in a Fragmented, Urbanizing Landscape.

    Directory of Open Access Journals (Sweden)

    T Winston Vickers

    Full Text Available Wide-ranging large carnivores pose myriad challenges for conservation, especially in highly fragmented landscapes. Over a 13-year period, we combined monitoring of radio collared pumas (Puma concolor with complementary multi-generational genetic analyses to inform puma conservation in southern California, USA. Our goals were to generate survivorship estimates, determine causes of mortality, identify barriers to movement, and determine the genetic and demographic challenges to puma persistence among >20,000,000 people and extensive urban, suburban, and exurban development. Despite protection from hunting, annual survival for radio collared pumas was surprisingly low (55.8%, and humans caused the majority of puma deaths. The most common sources of mortality were vehicle collisions (28% of deaths, and mortalities resulting from depredation permits issued after pumas killed domestic animals (17% of deaths. Other human-caused mortalities included illegal shootings, public safety removals, and human-caused wildfire. An interstate highway (I-15 bisecting this study area, and associated development, have created a nearly impermeable barrier to puma movements, resulting in severe genetic restriction and demographic isolation of the small puma population (n ~ 17-27 adults in the Santa Ana Mountains west of I-15. Highways that bisect habitat or divide remaining "conserved" habitat, and associated ongoing development, threaten to further subdivide this already fragmented puma population and increase threats to survival. This study highlights the importance of combining demographic and genetic analyses, and illustrates that in the absence of effective measures to reduce mortality and enhance safe movement across highways, translocation of pumas, such as was done with the endangered Florida panther (P. c. coryi, may ultimately be necessary to prevent further genetic decline and ensure persistence of the Santa Ana Mountains population.

  3. Fractured genetic connectivity threatens a southern california puma (Puma concolor population.

    Directory of Open Access Journals (Sweden)

    Holly B Ernest

    Full Text Available Pumas (Puma concolor; also known as mountain lions and cougars in southern California live among a burgeoning human population of roughly 20 million people. Yet little is known of the consequences of attendant habitat loss and fragmentation, and human-caused puma mortality to puma population viability and genetic diversity. We examined genetic status of pumas in coastal mountains within the Peninsular Ranges south of Los Angeles, in San Diego, Riverside, and Orange counties. The Santa Ana Mountains are bounded by urbanization to the west, north, and east, and are separated from the eastern Peninsular Ranges to the southeast by a ten lane interstate highway (I-15. We analyzed DNA samples from 97 pumas sampled between 2001 and 2012. Genotypic data for forty-six microsatellite loci revealed that pumas sampled in the Santa Ana Mountains (n = 42 displayed lower genetic diversity than pumas from nearly every other region in California tested (n = 257, including those living in the Peninsular Ranges immediately to the east across I-15 (n = 55. Santa Ana Mountains pumas had high average pairwise relatedness, high individual internal relatedness, a low estimated effective population size, and strong evidence of a bottleneck and isolation from other populations in California. These and ecological findings provide clear evidence that Santa Ana Mountains pumas have been experiencing genetic impacts related to barriers to gene flow, and are a warning signal to wildlife managers and land use planners that mitigation efforts will be needed to stem further genetic and demographic decay in the Santa Ana Mountains puma population.

  4. Fractured genetic connectivity threatens a southern california puma (Puma concolor) population.

    Science.gov (United States)

    Ernest, Holly B; Vickers, T Winston; Morrison, Scott A; Buchalski, Michael R; Boyce, Walter M

    2014-01-01

    Pumas (Puma concolor; also known as mountain lions and cougars) in southern California live among a burgeoning human population of roughly 20 million people. Yet little is known of the consequences of attendant habitat loss and fragmentation, and human-caused puma mortality to puma population viability and genetic diversity. We examined genetic status of pumas in coastal mountains within the Peninsular Ranges south of Los Angeles, in San Diego, Riverside, and Orange counties. The Santa Ana Mountains are bounded by urbanization to the west, north, and east, and are separated from the eastern Peninsular Ranges to the southeast by a ten lane interstate highway (I-15). We analyzed DNA samples from 97 pumas sampled between 2001 and 2012. Genotypic data for forty-six microsatellite loci revealed that pumas sampled in the Santa Ana Mountains (n = 42) displayed lower genetic diversity than pumas from nearly every other region in California tested (n = 257), including those living in the Peninsular Ranges immediately to the east across I-15 (n = 55). Santa Ana Mountains pumas had high average pairwise relatedness, high individual internal relatedness, a low estimated effective population size, and strong evidence of a bottleneck and isolation from other populations in California. These and ecological findings provide clear evidence that Santa Ana Mountains pumas have been experiencing genetic impacts related to barriers to gene flow, and are a warning signal to wildlife managers and land use planners that mitigation efforts will be needed to stem further genetic and demographic decay in the Santa Ana Mountains puma population.

  5. Survival and Mortality of Pumas (Puma concolor) in a Fragmented, Urbanizing Landscape.

    Science.gov (United States)

    Vickers, T Winston; Sanchez, Jessica N; Johnson, Christine K; Morrison, Scott A; Botta, Randy; Smith, Trish; Cohen, Brian S; Huber, Patrick R; Ernest, Holly B; Boyce, Walter M

    2015-01-01

    Wide-ranging large carnivores pose myriad challenges for conservation, especially in highly fragmented landscapes. Over a 13-year period, we combined monitoring of radio collared pumas (Puma concolor) with complementary multi-generational genetic analyses to inform puma conservation in southern California, USA. Our goals were to generate survivorship estimates, determine causes of mortality, identify barriers to movement, and determine the genetic and demographic challenges to puma persistence among >20,000,000 people and extensive urban, suburban, and exurban development. Despite protection from hunting, annual survival for radio collared pumas was surprisingly low (55.8%), and humans caused the majority of puma deaths. The most common sources of mortality were vehicle collisions (28% of deaths), and mortalities resulting from depredation permits issued after pumas killed domestic animals (17% of deaths). Other human-caused mortalities included illegal shootings, public safety removals, and human-caused wildfire. An interstate highway (I-15) bisecting this study area, and associated development, have created a nearly impermeable barrier to puma movements, resulting in severe genetic restriction and demographic isolation of the small puma population (n ~ 17-27 adults) in the Santa Ana Mountains west of I-15. Highways that bisect habitat or divide remaining "conserved" habitat, and associated ongoing development, threaten to further subdivide this already fragmented puma population and increase threats to survival. This study highlights the importance of combining demographic and genetic analyses, and illustrates that in the absence of effective measures to reduce mortality and enhance safe movement across highways, translocation of pumas, such as was done with the endangered Florida panther (P. c. coryi), may ultimately be necessary to prevent further genetic decline and ensure persistence of the Santa Ana Mountains population.

  6. La encrucijada del proceso andino de integración

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    Bernardo Vela O.

    2007-11-01

    Full Text Available El trabajo se divide en dos partes. La primera trata la relación entre integración e instituciones, y la segunda la relación entre integración y mundialización. En este sentido, la hipótesis que se prueba en el trabajo se propone como una paradoja: cuando más necesario se hace fortalecer el proceso de integración entre los países andinos, más precaria se torna la existencia de la

  7. The Role of Scent Marking in Mate Selection by Female Pumas (Puma concolor.

    Directory of Open Access Journals (Sweden)

    Maximilian L Allen

    Full Text Available Mate selection influences individual fitness, is often based on complex cues and behaviours, and can be difficult to study in solitary species including carnivores. We used motion-triggered cameras at 29 community scrapes (i.e. scent marking locations used by multiple individuals and home range data from 39 GPS-collared pumas (Puma concolor to assess the relevance of communication behaviours for mate selection by female pumas in California. Female pumas visited community scrapes irregularly and visitation bouts appeared to be correlated with oestrus. Female pumas on average selected from 1.7 collared males, and selection was based on multiple cues that varied among the different time periods measured (i.e. the female's visitation bout and in 90 days previous to the consorting event. Female mate selection over the course of a visitation bout was based on frequency of the male visitation, mass, and age. In the 90 days previous to consorting, the number of scrapes a male created was the most important contributor to selection, which was likely related to his residency status. We also found that at least 14% of females mated with multiple males, thus possibly confusing paternity. Our findings provide a mechanistic understanding of how female pumas use scent and auditory communication at community scrapes to select dominant resident males to mate with.

  8. Cycle and duration of the seminiferous epithelium in puma (Puma concolor).

    Science.gov (United States)

    Leite, Flaviana Lima Guião; de Paula, Tarcízio Antônio Rego; da Matta, Sérgio Luis Pinto; Fonseca, Cláudio Cesar; das Neves, Marco Túlio David; de Barros, João Bosco Gonçalves

    2006-02-01

    Puma or sussuarana (Puma concolor) is the second largest feline in the American continent and has an ample latitudinal distribution in very diverse habitats. In relation to its conservation status, the puma is considered an extinction-threatened species. The study of the testis morphology and the spermatogenic process in a species is fundamental for establishing the physiologic patterns that will make possible the selection of the protocols for assisted reproduction. A number of peculiarities associated with the reproductive biology of specific species such as the duration of spermatogenic process can be used to determine the frequency of sperm collection. Nine adult male pumas maintained in captivity were used to determine the relative frequency of stages in the seminiferous epithelium cycle. Three of them received intra-testicular injections of 0.1ml tritiated thymidine to determine the duration of the seminiferous epithelium cycle, and were subjected to biopsy 7 days later. The cycle of the seminiferous epithelium in puma was didactically described into eight stages by the tubular morphology method. The total duration of one seminiferous epithelium cycle in puma was calculated to be 9.89 days, and approximately 44.5 days are required for development of spermatozoon from spermatogonia. The duration of spermiogenesis, prophase and other events of meiosis were 14.08, 15.20 and 1.79 days, respectively. The relative frequency of the pre-meiotic, meiotic and post-meiotic phases were 3.98, 1.79 and 4.12 days, respectively.

  9. The Role of Scent Marking in Mate Selection by Female Pumas (Puma concolor).

    Science.gov (United States)

    Allen, Maximilian L; Wittmer, Heiko U; Houghtaling, Paul; Smith, Justine; Elbroch, L Mark; Wilmers, Christopher C

    2015-01-01

    Mate selection influences individual fitness, is often based on complex cues and behaviours, and can be difficult to study in solitary species including carnivores. We used motion-triggered cameras at 29 community scrapes (i.e. scent marking locations used by multiple individuals) and home range data from 39 GPS-collared pumas (Puma concolor) to assess the relevance of communication behaviours for mate selection by female pumas in California. Female pumas visited community scrapes irregularly and visitation bouts appeared to be correlated with oestrus. Female pumas on average selected from 1.7 collared males, and selection was based on multiple cues that varied among the different time periods measured (i.e. the female's visitation bout and in 90 days previous to the consorting event). Female mate selection over the course of a visitation bout was based on frequency of the male visitation, mass, and age. In the 90 days previous to consorting, the number of scrapes a male created was the most important contributor to selection, which was likely related to his residency status. We also found that at least 14% of females mated with multiple males, thus possibly confusing paternity. Our findings provide a mechanistic understanding of how female pumas use scent and auditory communication at community scrapes to select dominant resident males to mate with.

  10. Malignant paraganglioma in a cougar (Puma concolor).

    Science.gov (United States)

    Duhamelle, Alexis; Langlois, Isabelle; Pey, Pascaline; Tremblay, Josée; Ruel, Hélène; Parent, Joane; Lussier, Joanie; Doré, Monique

    2014-12-01

    A 7½-yr-old male cougar (Puma concolor) was presented with a 2-wk history of progressive hindlimb abnormalities. An abdominal mass was palpated on physical examination. Computed tomography of the abdomen showed a mass surrounding the left ureter. A postmortem diagnosis of paraganglioma was established.

  11. Puma predation on radiocollared and uncollared bighorn sheep

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    Johnson Christine K

    2009-11-01

    Full Text Available Abstract Background We used Global Positioning System (GPS data from radiocollared pumas (Puma concolor to identify kill sites of pumas preying upon an endangered population of bighorn sheep (Ovis canadensis in southern California. Our aims were to test whether or not pumas selected radiocollared versus uncollared bighorn sheep, and to identify patterns of movement before, during, and after kills. Findings Three pumas killed 23 bighorn sheep over the course of the study, but they did not preferentially prey on marked (radiocollared versus unmarked bighorn sheep. Predation occurred primarily during crepuscular and nighttime hours, and 22 kill sites were identified by the occurrence of 2 or more consecutive puma GPS locations (a cluster within 200 m of each other at 1900, 0000, and 0600 h. Conclusion We tested the "conspicuous individual hypothesis" and found that there was no difference in puma predation upon radiocollared and uncollared bighorn sheep. Pumas tended to move long distances before and after kills, but their movement patterns immediately post-kill were much more restricted. Researchers can exploit this behaviour to identify puma kill sites and investigate prey selection by designing studies that detect puma locations that are spatially clustered between dusk and dawn.

  12. c-Jun transactivates Puma gene expression to promote osteoarthritis.

    Science.gov (United States)

    Lu, Huading; Hou, Gang; Zhang, Yongkai; Dai, Yuhu; Zhao, Huiqing

    2014-05-01

    Osteoarthritis (OA) is a chronic degenerative joint disorder in which genetic, hormonal, mechanical and ageing factors affect its progression. Current studies are focusing on chondrocytes as a key mediator of OA at a cellular level. however, the mechanism underlying chondrocyte apoptosis remains unclear. PUMA is a pro-apoptotic member of the BH3-only subgroup of the Bcl-2 family and is involved in a large number of physiological and pathological processes. In the present study, we examined whether PUMA has a role in IL-1β-induced apoptosis and whether the c-Jun N-terminal kinase (JNK)/c-Jun pathway mediates the induction of PUMA, thus contributing to chondrocyte apoptosis. The results demonstrated an increase in PUMA protein and mRNA levels in cultured mouse chondrocytes following 4 h of IL-1β treatment. Furthermore, this upregulation of PUMA was critical for chondrocyte apoptosis as knockdown of PUMA using PUMA-specific siRNA significantly reduced apoptosis in cultured cells. Upon pharmacological inhibition of the JNK/c-Jun pathway with CE11004 or SP600125, the expression of PUMA was notably suppressed with a concomitant decrease in apoptosis observed in IL-1β-treated chondrocytes. Also, immunohistochemical studies revealed that the PUMA and c-Jun proteins were upregulated in chondrocytes from the articular cartilage of OA patients. Together, these data suggest a role for PUMA and the JNK/c-Jun pathway in the regulation of chondrocyte apoptosis during OA.

  13. Role of PUMA in methamphetamine-induced neuronal apoptosis.

    Science.gov (United States)

    Chen, Chuanxiang; Qincao, Litao; Xu, Jingtao; Du, Sihao; Huang, Enping; Liu, Chao; Lin, Zhoumeng; Xie, Wei-Bing; Wang, Huijun

    2016-01-05

    Exposure to methamphetamine (METH), a widely used illicit drug, has been shown to cause neuron apoptosis. p53 upregulated modulator of apoptosis (PUMA) is a key mediator in neuronal apoptosis. This study aimed to examine the effects of PUMA in METH-induced neuronal apoptosis. We determined PUMA protein expression in PC12 cells and SH-SY5Y cells after METH exposure using western blot. We also observed the effect of METH on neuronal apoptosis after silencing PUMA expression with siRNA using TUNEL staining and flow cytometry. Additionally, to investigate possible mechanisms of METH-induced PUMA-mediated neuronal apoptosis, we measured the protein expression of apoptotic markers, including cleaved caspase-3, cleaved PARP, Bax, B-cell leukemia/lymphoma-2 (Bcl-2) and cytochrome c (cyto c), after METH treatment with or without PUMA knockdown. Results showed that METH exposure induced cell apoptosis, increased PUMA protein levels, activated caspase-3 and PARP, elevated Bax and reduced Bcl-2 expression, as well as increased the release of cyto c from mitochondria to the cytoplasm in both PC12 and SH-SY5Y cells. All these effects were attenuated or reversed after silencing PUMA. A schematic depicting the role of PUMA in METH-induced mitochondrial apoptotic pathway was proposed. Our results suggest that PUMA plays an important role in METH-triggered apoptosis and it may be a potential target for ameliorating neuronal injury and apoptosis caused by METH.

  14. História, arte e política: o muralismo do boliviano Miguel Alandia Pantoja History, art and politics: the muralism of the Bolivian Miguel Alandia Pantoja

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    Everaldo de Oliveira Andrade

    2006-01-01

    Full Text Available Este artigo pretende avaliar em que medida arte e política se expressaram na criação e na ação do artista e militante político boliviano Miguel Alandia Pantoja, ativo participante de momentos centrais da História do seu país como a guerra do Chaco, a revolução de 1952 e a Comuna de La Paz de 1971. As influências do indigenismo andino e do muralismo mexicano parecem ter-se cruzado com as opções políticas de Alandia e com seu engajamento na proposta dos artistas ligados ao Manifesto por Uma Arte Revolucionária e Independente lançado por André Breton, Diego Rivera e Leon Trotski em 1936, permitindo o surgimento de uma obra original, polêmica e marcante para a História latino-americana.This article intends to evaluate how art and politics are expressed both in the creation and in the action of the Bolivian militant politician Miguel Alandia Pantoja, who was an active participant in decisive moments of his country's history, as the Chaco War, the 1952 Revolution and the Commune of La Paz in 1971. The Andean indigenismo and the Mexican muralist influences seemed to have crossed with Alandia political options and with his enrollment at the proposal made by artists related to the Manifesto for a Revolutionary and Independent Art launched by Andre Breton, Diego Rivera and Leon Trotski in 1936, allowing the uprising of an original and controversial artistic creation for Latin American history.

  15. Livestock Predation by Puma ( Puma concolor) in the Highlands of a Southeastern Brazilian Atlantic Forest

    Science.gov (United States)

    Palmeira, Francesca Belem Lopes; Trinca, Cristiano Trapé; Haddad, Claudio Maluf

    2015-10-01

    We evaluated local opinion about reducing livestock losses to puma ( Puma concolor) and the potential for conflict among livestock breeders inside a protected area in the highlands of a southeastern Brazilian Atlantic forest. We also quantified the number and type of livestock losses, and determined if predation by puma was correlated with property profile and landscape characteristics. We conducted semistructured interviews with 42 livestock breeders sampled in 36 rural properties. When asked how to reduce predation, 33 % of livestock breeders refused to answer, 26 % suggested improving livestock husbandry practices, 19 % stated that there was no appropriate action, 17 % favored removing the "problem" individual, and 5 % suggested killing the puma. Opinion on how to solve predation was independent of herd size and history of losses, and was correlated with respondent age class. Older respondents tended to suggest removing or killing pumas. Attitudes toward predation represented high potential for conflict among livestock breeders who demonstrated high discordance among responses. Horses were the most common prey (51 %), followed by cattle (28 %), sheep (17 %), and goats (4 %); totaling 47 animals attacked between 2004 and 2007. Annual predation was approximately 12 ± 5 animals, equivalent to 0.4 % of the total livestock. Property elevation and distance from the urban center were the main predictors of predation probability. This survey used a novel approach that has not been addressed directly in other studies on livestock predation and demonstrated that the high potential for conflict among livestock breeders should be considered before implementing management actions.

  16. Food habits of pumas in northwestern Sonora, Mexico

    Science.gov (United States)

    Rosas-Rosas, O. C.; Valdez, R.; Bender, L.C.; Daniel, D.

    2003-01-01

    It is questionable whether food-habits studies of pumas conducted in the southwestern United States can be extrapolated to northwestern Mexico, because of differences in management, distribution, and abundance of wildlife. We determined food habits of pumas (Puma concolor) in the Sonoran Desert of northwestern Sonora, Mexico. Based on studies in the western United States, we hypothesized that desert mule deer (Odocoileus hemionus) were the major food source of pumas in Sonoran Desert habitats of Mexico. The study area supports populations of desert mule deer, white-tailed deer (Odocoileus virginianus), lagomorphs (Lepus spp. and Sylvilagus audubonii), collared peccary (Pecari tajacu), and the largest population (???300 individuals) of desert bighorn sheep (Ovis canadensis) in Sonora. Based on pugmark characteristics, we recorded 3 different adult resident pumas in approximately 90 km2. We analyzed 60 puma fecal samples collected September 1996-November 1998. Primary prey items based on frequency of occurrence and estimated biomass consumed were desert bighorn sheep (40% and 45%, respectively), lagomorphs (33%, 19%), deer (17%, 17%), and collared peccary (15%, 11%). The high percentage of desert bighorn sheep in puma diets may be due to high abundance relative to mule deer, which declined in number during our study. No differences were found in puma diets between seasons (??22=2.4526, P=0.2934). Fluctuations in mule deer populations in northwestern Sonora may influence prey selection by pumas.

  17. Crizotinib induces PUMA-dependent apoptosis in colon cancer cells.

    Science.gov (United States)

    Zheng, Xingnan; He, Kan; Zhang, Lin; Yu, Jian

    2013-05-01

    Oncogenic alterations in MET or anaplastic lymphoma kinase (ALK) have been identified in a variety of human cancers. Crizotinib (PF02341066) is a dual MET and ALK inhibitor and approved for the treatment of a subset of non-small cell lung carcinoma and in clinical development for other malignancies. Crizotinib can induce apoptosis in cancer cells, whereas the underlying mechanisms are not well understood. In this study, we found that crizotinib induces apoptosis in colon cancer cells through the BH3-only protein PUMA. In cells with wild-type p53, crizotinib induces rapid induction of PUMA and Bim accompanied by p53 stabilization and DNA damage response. The induction of PUMA and Bim is mediated largely by p53, and deficiency in PUMA or p53, but not Bim, blocks crizotinib-induced apoptosis. Interestingly, MET knockdown led to selective induction of PUMA, but not Bim or p53. Crizotinib also induced PUMA-dependent apoptosis in p53-deficient colon cancer cells and synergized with gefitinib or sorafenib to induce marked apoptosis via PUMA in colon cancer cells. Furthermore, PUMA deficiency suppressed apoptosis and therapeutic responses to crizotinib in xenograft models. These results establish a critical role of PUMA in mediating apoptotic responses of colon cancer cells to crizotinib and suggest that mechanisms of oncogenic addiction to MET/ALK-mediated survival may be cell type-specific. These findings have important implications for future clinical development of crizotinib.

  18. La escolaridad como hito en las biografías de los hijos de bolivianos en Buenos Aires

    Directory of Open Access Journals (Sweden)

    Natalia Gavazzo

    Full Text Available Este trabajo se propone un dialogo entre tres trabajos de investigación realizados desde perspectivas socio antropológicas aplicadas al análisis de las experiencias de los hijos de inmigrantes bolivianos en distintos puntos de la Ciudad de Buenos Aires y la Provincia de Buenos Aires. A partir del estudio de los procesos migratorios desde Bolivia y de sus cambios a lo largo de las generaciones, pretendemos postular que la escolaridad constituye parte fundamental de la experiencia de los niños y jóvenes migrantes y muy especialmente de los descendientes de inmigrantes bolivianos. Puesto que la misma afecta su formación intelectual, pero sobre todo, sus identificaciones nacionales, étnicas, de género, de clase y particularmente de edad, nos enfocaremos en comprender el rol de la escolaridad en esa etapa vital considerada como juventud. Teniendo en cuenta algunas de las representaciones mas habituales en los casos examinados, expondremos que el paso por la escuela primaria y secundaria implica un hito en las biografías de los hijos de los bolivianos y los niños y jóvenes bolivianos que se han criado en Buenos Aires y analizaremos el impacto en sus formas de identificación y sus relaciones.

  19. El impacto del TLC frente al derecho comunitario andino

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    Marcel Tangarife Torres

    2006-11-01

    Full Text Available En esta conferencia analiza el impacto del Tratado de Libre Comercio que se celebrará entre la República de Colombia y los Estados Unidos de Norteamérica frente al Derecho Comunitario Andino, con gran importancia en los temas relacionados con la propiedad intelectual. Adicional a lo anterior se suma un aspecto trascendental y es la supervivencia de la Comunidad Andina de Naciones y en este sentido se cuestiona la coexistencia de las normas comunitarias en relación con las previstas en el Tratado de Libre Comercio. De esta manera, se examina el marco constitucional del Tratado de Libre Comercio y del Acuerdo de Cartagena, para así determinar las características que tienen esta clase de tratados y con ello establecer el nivel jerárquico que le corresponden dentro del ordenamiento jurídico Colombiano. Finalmente se observa la prevalencía de las normas del Acuerdo de Cartagena y el Tratado de Libre Comercio o viceversa, para con ello encontrar soluciones frente a los posibles conflictos de normas que se pueden presentar por la coexistencia de dichas normatividades.

  20. El impacto del TLC frente al derecho comunitario andino

    Directory of Open Access Journals (Sweden)

    Marcel Tangarife Torres

    2006-01-01

    Full Text Available En esta conferencia analiza el impacto del Tratado de Libre Comercio que se celebrará entre la República de Colombia y los Estados Unidos de Norteamérica frente al Derecho Comunitario Andino, con gran importancia en los temas relacionados con la propiedad intelectual. Adicional a lo anterior se suma un aspecto trascendental y es la supervivencia de la Comunidad Andina de Naciones y en este sentido se cuestiona la coexistencia de las normas comunitarias en relación con las previstas en el Tratado de Libre Comercio. De esta manera, se examina el marco constitucional del Tratado de Libre Comercio y del Acuerdo de Cartagena, para así determinar las características que tienen esta clase de tratados y con ello establecer el nivel jerárquico que le corresponden dentro del ordenamiento jurídico Colombiano. Finalmente se observa la prevalencía de las normas del Acuerdo de Cartagena y el Tratado de Libre Comercio o viceversa, para con ello encontrar soluciones frente a los posibles conflictos de normas que se pueden presentar por la coexistencia de dichas normatividades.

  1. Molecular evidence for a recent demographic expansion in the puma (Puma concolor (Mammalia, Felidae

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    Eunice M. Matte

    2013-01-01

    Full Text Available The puma is an iconic predator that ranges throughout the Americas, occupying diverse habitats. Previous phylogeographic analyses have revealed that it exhibits moderate levels of genetic structure across its range, with few of the classically recognized subspecies being supported as distinct demographic units. Moreover, most of the species' molecular diversity was found to be in South America. To further investigate the phylogeographic structure and demographic history of pumas we analyzed mtDNA sequences from 186 individuals sampled throughout their range, with emphasis on South America. Our objectives were to refine the phylogeographic assessment within South America and to investigate the demographic history of pumas using a coalescent approach. Our results extend previous phylogeographic findings, reassessing the delimitation of historical population units in South America and demonstrating that this species experienced a considerable demographic expansion in the Holocene, ca. 8,000 years ago. Our analyses indicate that this expansion occurred in South America, prior to the hypothesized re-colonization of North America, which was therefore inferred to be even more recent. The estimated demographic history supports the interpretation that pumas suffered a severe demographic decline in the Late Pleistocene throughout their distribution, followed by population expansion and re-colonization of the range, initiating from South America.

  2. Models of regional habitat quality and connectivity for pumas (Puma concolor in the southwestern United States.

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    Brett G Dickson

    Full Text Available The impact of landscape changes on the quality and connectivity of habitats for multiple wildlife species is of global conservation concern. In the southwestern United States, pumas (Puma concolor are a well distributed and wide-ranging large carnivore that are sensitive to loss of habitat and to the disruption of pathways that connect their populations. We used an expert-based approach to define and derive variables hypothesized to influence the quality, location, and permeability of habitat for pumas within an area encompassing the entire states of Arizona and New Mexico. Survey results indicated that the presence of woodland and forest cover types, rugged terrain, and canyon bottom and ridgeline topography were expected to be important predictors of both high quality habitat and heightened permeability. As road density, distance to water, or human population density increased, the quality and permeability of habitats were predicted to decline. Using these results, we identified 67 high quality patches across the study area, and applied concepts from electronic circuit theory to estimate regional patterns of connectivity among these patches. Maps of current flow among individual pairs of patches highlighted possible pinch points along two major interstate highways. Current flow summed across all pairs of patches highlighted areas important for keeping the entire network connected, regardless of patch size. Cumulative current flow was highest in Arizona north of the Colorado River and around Grand Canyon National Park, and in the Sky Islands region owing to the many small habitat patches present. Our outputs present a first approximation of habitat quality and connectivity for dispersing pumas in the southwestern United States. Map results can be used to help target finer-scaled analyses in support of planning efforts concerned with the maintenance of puma metapopulation structure, as well as the protection of landscape features that facilitate

  3. Models of regional habitat quality and connectivity for pumas (Puma concolor) in the southwestern United States.

    Science.gov (United States)

    Dickson, Brett G; Roemer, Gary W; McRae, Brad H; Rundall, Jill M

    2013-01-01

    The impact of landscape changes on the quality and connectivity of habitats for multiple wildlife species is of global conservation concern. In the southwestern United States, pumas (Puma concolor) are a well distributed and wide-ranging large carnivore that are sensitive to loss of habitat and to the disruption of pathways that connect their populations. We used an expert-based approach to define and derive variables hypothesized to influence the quality, location, and permeability of habitat for pumas within an area encompassing the entire states of Arizona and New Mexico. Survey results indicated that the presence of woodland and forest cover types, rugged terrain, and canyon bottom and ridgeline topography were expected to be important predictors of both high quality habitat and heightened permeability. As road density, distance to water, or human population density increased, the quality and permeability of habitats were predicted to decline. Using these results, we identified 67 high quality patches across the study area, and applied concepts from electronic circuit theory to estimate regional patterns of connectivity among these patches. Maps of current flow among individual pairs of patches highlighted possible pinch points along two major interstate highways. Current flow summed across all pairs of patches highlighted areas important for keeping the entire network connected, regardless of patch size. Cumulative current flow was highest in Arizona north of the Colorado River and around Grand Canyon National Park, and in the Sky Islands region owing to the many small habitat patches present. Our outputs present a first approximation of habitat quality and connectivity for dispersing pumas in the southwestern United States. Map results can be used to help target finer-scaled analyses in support of planning efforts concerned with the maintenance of puma metapopulation structure, as well as the protection of landscape features that facilitate the dispersal

  4. Esophageal stricture in a cougar (Puma concolor).

    Science.gov (United States)

    Desmarchelier, Marion; Lair, Stéphane; Defarges, Alice; Lécuyer, Manon; Langlois, Isabelle

    2009-06-01

    A 7-mo-old female cougar (Puma concolor) was presented with a 2-wk history of anorexia and a 1-wk history of regurgitation. Barium contrast esophagogram and gastroesophagoscopy revealed the presence of a segmental intraluminal esophageal stricture in the middle third of the esophagus. The stricture was potentially secondary to a previous anesthetic episode. Three endoscopic balloon dilations allowed increasing the luminal diameter to a size that enabled the cougar to eat food softened with water without any signs of discomfort or regurgitation. Two months after being discharged, the cougar was doing well, had gained weight and was eating horsemeat softened with water.

  5. El proceso constituyente boliviano como escenario de disputas por la economía

    Directory of Open Access Journals (Sweden)

    Milena González Piñeros

    2011-04-01

    Full Text Available Este artículo analiza el reciente proceso constituyente boliviano como un escenario de disputas sociales y políticas por el tipo de economía por plasmar en la Nueva Constitución. En la primera parte se hace referencia al entramado monocultural, colonial y excluyente que ha sostenido una forma de entender la economía, la cual ha estado respaldada por la Constitución en favor de un bloque específico de poder. En la segunda parte, el escenario constituyente se analiza como oportunidad histórica para debatir el sentido de la economía. Como resultado de esa disputa, surge el modelo económico nacional productivo dentro del texto constitucional.

  6. Intralesional vincristine use for treatment of squamous cell carcinoma in a puma (Puma concolor).

    Science.gov (United States)

    Sandoval, Blanca Juarez; Amat, Azlan Che'; Sabri, Jasni; Ramli, Mat Naim

    2013-12-01

    A 14-yr-old male puma (Puma concolor) was presented to the veterinary staff of the National Zoo in Malaysia for an auricular mass. Squamous cell carcinoma was diagnosed by histologic examination of a biopsy. Systemic administration of chemotherapy using vincristine (0.5 mg/m2 i.v. q. 7 days for six treatments) and prednisolone (2 mg/kg i.m. q. 72 hr x 7 days) caused side effects of vomiting, weight loss, and alopecia and did not improve the size or appearance of the tumor. Intralesional vincristine injections (0.2 mg q. 7 days for two treatments) and prednisolone (2 mg/kg i.m. q. 72 hr x 15 days) were administered, resulting in complete tumor regression after 14 days of treatment.

  7. PUMA mediates ER stress-induced apoptosis in portal hypertensive gastropathy.

    Science.gov (United States)

    Tan, S; Wei, X; Song, M; Tao, J; Yang, Y; Khatoon, S; Liu, H; Jiang, J; Wu, B

    2014-03-13

    Mucosal apoptosis has been demonstrated to be an essential pathological feature in portal hypertensive gastropathy (PHG). p53-upregulated modulator of apoptosis (PUMA) was identified as a BH3-only Bcl-2 family protein that has an essential role in apoptosis induced by a variety of stimuli, including endoplasmic reticulum (ER) stress. However, whether PUMA is involved in mucosal apoptosis in PHG remains unclear, and whether PUMA induces PHG by mediating ER stress remains unknown. The aim of the study is to investigate whether PUMA is involved in PHG by mediating ER stress apoptotic signaling. To identify whether PUMA is involved in PHG by mediating ER stress, gastric mucosal injury and apoptosis were studied in both PHG patients and PHG animal models using PUMA knockout (PUMA-KO) and PUMA wild-type (PUMA-WT) mice. The induction of PUMA expression and ER stress signaling were investigated, and the mechanisms of PUMA-mediated apoptosis were analyzed. GES-1 and SGC7901 cell lines were used to further identify whether PUMA-mediated apoptosis was induced by ER stress in vitro. Epithelial apoptosis and PUMA were markedly induced in the gastric mucosa of PHG patients and mouse PHG models. ER stress had a potent role in the induction of PUMA and apoptosis in PHG models, and the apoptosis was obviously attenuated in PUMA-KO mice. Although the targeted deletion of PUMA did not affect ER stress, mitochondrial apoptotic signaling was downregulated in mice. Meanwhile, PUMA knockdown significantly ameliorated ER stress-induced mitochondria-dependent apoptosis in vitro. These results indicate that PUMA mediates ER stress-induced mucosal epithelial apoptosis through the mitochondrial apoptotic pathway in PHG, and that PUMA is a potentially therapeutic target for PHG.

  8. Remesas a los países andinos: Tendencias, costos e impacto económico

    OpenAIRE

    Andrés Solimano

    2003-01-01

    Da a conocer las tendencias globales y regionales de las remesas en los países andinos, las características de los mercados internacionales, costos del envío de remesas y las políticas para reducir costos de envío de remesas.

  9. A new panel of SNP markers for the individual identification of North American pumas

    Science.gov (United States)

    Fitak, Robert R.; Naidu, Ashwin; Thompson, Ron W.; Culver, Melanie

    2016-01-01

    Pumas Puma concolor are one of the most studied terrestrial carnivores because of their widespread distribution, substantial ecological impacts, and conflicts with humans. Over the past decade, managing pumas has involved extensive efforts including the use of genetic methods. Microsatellites have been the most commonly used genetic markers; however, technical artifacts and little overlap of frequently used loci render large-scale comparison of puma genetic data across studies challenging. Therefore, a panel of genetic markers that can produce consistent genotypes across studies without the need for extensive calibrations is essential for range-wide genetic management of puma populations. Here, we describe the development of PumaPlex, a high-throughput assay to genotype 25 single nucleotide polymorphisms in pumas. We validated PumaPlex in 748 North American pumas Puma concolor couguar, and demonstrated its ability to generate reproducible genotypes and accurately identify individuals. Furthermore, in a test using fecal deoxyribonucleic acid (DNA) samples, we found that PumaPlex produced significantly more genotypes with fewer errors than 12 microsatellite loci, 8 of which are commonly used. Our results demonstrate that PumaPlex is a valuable tool for the genetic monitoring and management of North American puma populations. Given the analytical simplicity, reproducibility, and high-throughput capability of single nucleotide polymorphisms, PumaPlex provides a standard panel of markers that promotes the comparison of genotypes across studies and independent of the genotyping technology used.

  10. PUMA: An Operating System for Massively Parallel Systems

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    Stephen R. Wheat

    1994-01-01

    Full Text Available This article presents an overview of PUMA (Performance-oriented, User-managed Messaging Architecture, a message-passing kernel for massively parallel systems. Message passing in PUMA is based on portals – an opening in the address space of an application process. Once an application process has established a portal, other processes can write values into the portal using a simple send operation. Because messages are written directly into the address space of the receiving process, there is no need to buffer messages in the PUMA kernel and later copy them into the applications address space. PUMA consists of two components: the quintessential kernel (Q-Kernel and the process control thread (PCT. Although the PCT provides management decisions, the Q-Kernel controls access and implements the policies specified by the PCT.

  11. PUMA-PCCS separate effect tests and RELAP5 code evaluation in PUMA

    Science.gov (United States)

    Choi, Sung Won

    One of the key areas in the design of advanced nuclear reactors is to develop a reliable Passive Containment Cooling System (PCCS). The purpose of the current work is to better understand the condensation phenomena in PCCS for the downward co-current flow of a steam/air mixture through condenser tube bundles during the three PCCS operational modes, namely the bypass mode, the cyclic venting mode and the long-term cooling mode. A series of unique separate-effect PCCS test data were obtained for condensation heat transfer in the PCCS heat exchangers of the PUMA (Purdue University Multidimensional Integral Test Assembly) facility under a task sponsored by the U.S. Nuclear Regulatory Commission. Test conditions includes bypass mode, cyclic venting mode and long term mode, covering a wide range of Loss of Coolant Accident(LOCA) conditions with a parameters of pressure, mass flow rate, noncondensable(NC) gases, and PCCS pool water level. The parametric effect studies and a further validation of the PUMA-PCCS separate effect test data were performed. The evaluation of a best estimate system code (RELAP5/MOD3.3) was performed by using unique PUMA-PCCS separate effects data and PUMA-Main Steam Line Break (MSLB) integral test (1998). Through a sensitivity studies of nodalization method and physical models on the MSLB test simulations, deficiencies in RELAP5/MOD3.3 code were found as follows: (1) over prediction of heat removal rate by condensation models, (2) overestimation of SP heat transfer through the horizontal venting line and thermal stratification distortion, (3) underestimation of NC gas effects in PCCS by the distortion of cyclic venting phenomena and (4) overestimation of the DW and SP wall condensation. The improvement for the code calculation predictions could be obtained by removing the RELAP5/MOD3.3 code deficient factors in the PUMA MSLB integral test simulation. The unique PCCS NC gas venting visualizations were obtained according to various PCCS inlet NC

  12. Innovative optronics for the new PUMA tank

    Science.gov (United States)

    Fritze, J.; Münzberg, M.; Schlemmer, H.

    2010-04-01

    The new PUMA tank is equipped with a fully stabilized 360° periscope. The thermal imager in the periscope is identical to the imager in the gunner sight. All optronic images of the cameras can be fed on every electronic display within the tank. The thermal imagers operate with a long wave 384x288 MCT starring focal plane array. The high quantum efficiency of MCT provides low NETD values at short integration times. The thermal imager has an image resolution of 768x576 pixels by means of a micro scanner. The MCT detector operates at high temperatures above 75K with high stability in noise and correctibility and offers high reliability (MTTF) values for the complete camera in a very compact design. The paper discusses the principle and functionality of the optronic combination of direct view optical channel, thermal imager and visible camera and discusses in detail the performances of the subcomponents with respect to demands for new tank applications.

  13. PUMA Pædagogisk udredningsmateriale til AKT - og specialundervisning

    DEFF Research Database (Denmark)

    Kristensen, René

    2011-01-01

    PUMA er et nyudviklet pædagogisk udredningsmateriale bestående af en bog samt en cd med 35 opgaver, modeller og skemaer til udskrivning på skolelicensvilkår. Materialet arbejder ud fra anerkendte teorier som systemisk konstruktionisme, selvpsykologi, samt kognitive teorier om hukommelse, analyse og...... lignende. Der kan downloades en flyer, samt supplerende materiale fra www.rkris.dk/PUMA...

  14. Idelalisib induces PUMA-dependent apoptosis in colon cancer cells.

    Science.gov (United States)

    Yang, Shida; Zhu, Zhiyong; Zhang, Xiaobing; Zhang, Ning; Yao, Zhicheng

    2017-01-24

    Idelalisib, a PI3K inhibitor, specifically targeting p110δ, has been approved for the treatment of chronic lymphocytic leukemia/small lymphocytic lymphoma and follicular lymphoma. However, the mechanisms of action of idelalisib in colon cancer cells are not well understood. We investigated how idelalisib suppresses colon cancer cells growth and potentiates effects of other chemotherapeutic drugs. In this study, we found that idelalisib treatment induces PUMA in colon cancer cells irrespective of p53 status through the p65 pathway following AKT inhibition and glycogen synthase kinase 3β (GSK3β) activation. PUMA is necessary for idelalisib-induced apoptosis in colon cancer cells. Idelalisib also synergized with 5-FU or regorafenib to induce marked apoptosis via PUMA in colon cancer cells. Furthermore, PUMA deficiency suppressed apoptosis and antitumor effect of idelalisib in xenograft model. These results demonstrate a critical role of PUMA in mediating the anticancer effects of idelalisib in colon cancer cells and suggest that PUMA induction can be used as an indicator of idelalisib sensitivity, and also have important implications for it clinical applications.

  15. Caspase-9 mediates Puma activation in UCN-01-induced apoptosis.

    Science.gov (United States)

    Nie, C; Luo, Y; Zhao, X; Luo, N; Tong, A; Liu, X; Yuan, Z; Wang, C; Wei, Y

    2014-10-30

    The protein kinase inhibitor 7-hydroxystaurosporine (UCN-01) is one of the most potent and frequently used proapoptotic stimuli. The BH3-only molecule of Bcl-2 family proteins has been reported to contribute to UCN-01-induced apoptosis. Here we have found that UCN-01 triggers Puma-induced mitochondrial apoptosis pathway. Our data confirmed that Akt-FoxO3a pathway mediated Puma activation. Importantly, we elucidate the detailed mechanisms of Puma-induced apoptosis. Our data have also demonstrated that caspase-9 is a decisive molecule of Puma induction after UCN-01 treatment. Caspase-9 mediates apoptosis through two kinds of feedback loops. On the one hand, caspase-9 enhances Puma activation by cleaving Bcl-2 and Bcl-xL independent of caspase-3. On the other hand, caspase-9 directly activated caspase-3 in the presence of caspase-3. Caspase-3 could cleave XIAP in an another positive feedback loop to further sensitize cancer cells to UCN-01-induced apoptosis. Therefore, caspase-9 mediates Puma activation to determine the threshold for overcoming chemoresistance in cancer cells.

  16. HER2 phosphorylates and destabilizes pro-apoptotic PUMA, leading to antagonized apoptosis in cancer cells.

    Science.gov (United States)

    Carpenter, Richard L; Han, Woody; Paw, Ivy; Lo, Hui-Wen

    2013-01-01

    HER2 is overexpressed in 15-20% of breast cancers. HER2 overexpression is known to reduce apoptosis but the underlying mechanisms for this association remain unclear. To elucidate the mechanisms for HER2-mediated survival, we investigated the relationship between HER2 and p53 upregulated modulator of apoptosis (PUMA), a potent apoptosis inducer. Our results showed that HER2 interacts with PUMA, which was independent of HER2 activation. In addition, we observed that HER2 interacted with PUMA in both mitochondrial and non-mitochondrial compartments. We next examined whether HER2 phosphorylates PUMA. Notably, PUMA tyrosine phosphorylation has never been reported. Using an intracellular assay, we found PUMA to be phosphorylated in breast cancer cells with activated HER2. Via cell-free HER2 kinase assay, we observed that PUMA was directly phosphorylated by HER2. Activation of HER2 decreased PUMA protein half-life. To identify which of the three tyrosines within PUMA are targeted by HER2, we generated three PUMA non-phosphorylation mutants each with a single Tyr→Phe substitution. Results indicated that each PUMA single mutant had lost some, but not all phosphorylation by HER2 indicating that HER2 targets all three tyrosines. Consequently, we created an additional PUMA mutant with all three tyrosines mutated (TM-PUMA) that could not be phosphorylated by HER2. Importantly, TM-PUMA was found to have a longer half-life than PUMA. An inverse association was observed between HER2 and PUMA in 93 invasive breast carcinoma samples. We further found that TM-PUMA suppressed growth of breast cancer cells to a greater degree than PUMA. Also, TM-PUMA had a stronger propensity to induce apoptosis than PUMA. Together, our results demonstrate, for the first time, that PUMA can be tyrosine phosphorylated and that HER2-mediated phosphorylation destabilizes PUMA protein. The HER2-PUMA interplay represents a novel mechanism by which PUMA is regulated and a new molecular basis for HER2

  17. Por quê confiamos nas instituições? O caso boliviano

    Directory of Open Access Journals (Sweden)

    Vivian Schwarz-Blum

    2006-11-01

    Full Text Available A Bolívia passou por um grande processo de reforma institucional desde seu retorno à democracia, em 1982, reconhecido internacionalmente como um dos mais profundos da América Latina. Apesar de todos os esforços do Estado boliviano para aperfeiçoar e modernizar suas instituições, e apesar das reformas bem sucedidas em setores fundamentais do governo, a Bolívia tem um dos níveis mais baixos de confiança nas instituições dos países latino-americanos e os dados mostram indícios de que a confiança nas instituições políticas tende a diminuir com o tempo. Ao mesmo tempo, a Bolívia é o país mais pobre da América do Sul; tem os graus mais altos de corrupção na administração pública e, nos últimos anos, um ambiente político cada vez mais instável. Há indícios de que os efeitos de uma polarização regional de interesses, as percepções negativas da situação econômica e da corrupção e níveis baixos de conhecimento político da população boliviana sejam preditores confiáveis dos baixos níveis de confiança institucional que consistentemente encobrem a melhoria do desempenho institucional dos governos bolivianos.Bolivia has undergone a deep process of institutional reform since its return to democracy in 1982 that is recognized internationally as one of the deepest in the Latin American region. Despite all the efforts of the Bolivian state to improve and modernize its institutions, Bolivia has one of the lowest levels of institutional trust among Latin American countries and data show evidence that trust in political institutions tends to decrease over time. At the same time, Bolivia is the poorest country in South America; it has high levels of corruption in the public administration and in the last years an increasingly unstable political environment. Evidence indicates that the effects of a regional polarization of interests, the negative perceptions of the economic situation and of corruption and low levels of

  18. Nuisance ecology: do scavenging condors exact foraging costs on pumas in Patagonia?

    Directory of Open Access Journals (Sweden)

    L Mark Elbroch

    Full Text Available Predation risk describes the energetic cost an animal suffers when making a trade off between maximizing energy intake and minimizing threats to its survival. We tested whether Andean condors (Vultur gryphus influenced the foraging behaviors of a top predator in Patagonia, the puma (Puma concolor, in ways comparable to direct risks of predation for prey to address three questions: 1 Do condors exact a foraging cost on pumas?; 2 If so, do pumas exhibit behaviors indicative of these risks?; and 3 Do pumas display predictable behaviors associated with prey species foraging in risky environments? Using GPS location data, we located 433 kill sites of 9 pumas and quantified their kill rates. Based upon time pumas spent at a carcass, we quantified handling time. Pumas abandoned >10% of edible meat at 133 of 266 large carcasses after a single night, and did so most often in open grasslands where their carcasses were easily detected by condors. Our data suggested that condors exacted foraging costs on pumas by significantly decreasing puma handling times at carcasses, and that pumas increased their kill rates by 50% relative to those reported for North America to compensate for these losses. Finally, we determined that the relative risks of detection and associated harassment by condors, rather than prey densities, explained puma "giving up times" (GUTs across structurally variable risk classes in the study area, and that, like many prey species, pumas disproportionately hunted in high-risk, high-resource reward areas.

  19. El "Tío" está sordo: Los mineros bolivianos y el Patrimonio Cultural Inmaterial

    Directory of Open Access Journals (Sweden)

    Gerardo Fernández Juárez

    2013-09-01

    Full Text Available Los mineros quechuas de Potosí rinden culto en el interior de mina a un personaje al que denominan “Tío”, el diablo. El artículo reflexiona sobre las contradicciones aparentes en que incurre la UNESCO en el reconocimiento de los bienes de interés cultural intangible o inmaterial aplicadas, en este caso, a la figura del “Tío” y su contexto minero. Las perspectivas “emic” y “etic” chocan con especial violencia intelectual al valorar la significación cultural del “Tio” en las minas potosinas como bien cultural intangible, en un contexto social difícilmente compatible con la Declaración Universal de los Derechos Humanos, tal y como reclama la UNESCO. ¿La cultura de la pobreza en que viven los mineros bolivianos agudiza la responsabilidad ética del “tío” o son los responsables del turismo en la zona los que usan al tío para “invisibilizar” a los propios mineros? El artículo pretende responder a estas cuestiones y acercarnos a la vez a las vivencias y expresiones que sobre el “tío” realizan los propios mineros, y analizar las profundas contradicciones que la UNESCO presenta en sus criterios de definición ,” puesta en valor” y reconocimiento de los bienes del Patrimonio Cultural inmaterial.

  20. El peso de lo institucional: auge y caída del modelo boliviano

    Directory of Open Access Journals (Sweden)

    Simón PACHANO

    2009-11-01

    Full Text Available RESUMEN: Este artículo debate la relación entre el tipo de régimen y las orientaciones de la política. Analiza las características institucionales que han llevado a considerar al caso boliviano como una excepción dentro del presidencialismo predominante en América Latina. A la modalidad de elección presidencial, que ha sido considerada elemento básico en ese aspecto, añade dos componentes del diseño institucional que tienen efectos sobre los cálculos estratégicos de los actores y consecuentemente sobre la construcción de consensos. Finalmente, busca detectar la incidencia de esas mismas disposiciones institucionales en el agotamiento del modelo consensual y de coaliciones que estuvo vigente por casi dos décadas.ABSTRACT: This article examines the relationship between regime type and political orientation. It analyzes the institutional features that make Bolivia an exception within the context of presidentialism in Latin America. In addition to the system for the election of the president, which has been considered the cornerstone of the political system, there are tow other features of institutional design which have effects on the strategic calculations of the political actors, and consequently, also for consensus building. Finally, the article addresses how these same features have impacted on the decline of the consensus and coalition based political model that has prevailed for almost two decades.

  1. Estructuras agrarias comparadas: la Puna argentina y el sur boliviano a comienzos del siglo XX

    Directory of Open Access Journals (Sweden)

    Ana A. Teruel

    2005-01-01

    Full Text Available This article expects to be another contribution to the knowledge about the social-economic problems from the Argentine Puna, especifically to its agricultural structure, paying special attention to changes and permanences, concerning to property and land, key to economical and social power and fundamental axis of production relationship of this society. It focuses on the problematical fact of indian lands, making a comparison between the North of Argentina and the South of Bolivia, considering both political state systems about comunity lands and its effects in the agrarian structures in the begining of the XXth century.Este artículo tiene por propósito realizar un aporte más al conocimiento de la problemática socio-económica de la Puna argentina, específicamente a su estructura agraria, atendiendo los cambios y permanencias relativos a la propiedad y tenencia de la tierra, clave del poder económico y social y eje fundamental en las relaciones de producción de esta sociedad agraria. Se trata la cuestión en el marco de la problemática de las tierras indígenas, comparando la situación del Norte argentino con la del Sur boliviano, analizando las políticas de ambos Estados en torno a la tierra de las comunidades y sus efectos en las estructuras agrarias de comienzos del siglo XX.

  2. Evolution of puma lentivirus in bobcats (Lynx rufus) and mountain lions (Puma concolor) in North America.

    Science.gov (United States)

    Lee, Justin S; Bevins, Sarah N; Serieys, Laurel E K; Vickers, Winston; Logan, Ken A; Aldredge, Mat; Boydston, Erin E; Lyren, Lisa M; McBride, Roy; Roelke-Parker, Melody; Pecon-Slattery, Jill; Troyer, Jennifer L; Riley, Seth P; Boyce, Walter M; Crooks, Kevin R; VandeWoude, Sue

    2014-07-01

    Mountain lions (Puma concolor) throughout North and South America are infected with puma lentivirus clade B (PLVB). A second, highly divergent lentiviral clade, PLVA, infects mountain lions in southern California and Florida. Bobcats (Lynx rufus) in these two geographic regions are also infected with PLVA, and to date, this is the only strain of lentivirus identified in bobcats. We sequenced full-length PLV genomes in order to characterize the molecular evolution of PLV in bobcats and mountain lions. Low sequence homology (88% average pairwise identity) and frequent recombination (1 recombination breakpoint per 3 isolates analyzed) were observed in both clades. Viral proteins have markedly different patterns of evolution; sequence homology and negative selection were highest in Gag and Pol and lowest in Vif and Env. A total of 1.7% of sites across the PLV genome evolve under positive selection, indicating that host-imposed selection pressure is an important force shaping PLV evolution. PLVA strains are highly spatially structured, reflecting the population dynamics of their primary host, the bobcat. In contrast, the phylogeography of PLVB reflects the highly mobile mountain lion, with diverse PLVB isolates cocirculating in some areas and genetically related viruses being present in populations separated by thousands of kilometers. We conclude that PLVA and PLVB are two different viral species with distinct feline hosts and evolutionary histories. Importance: An understanding of viral evolution in natural host populations is a fundamental goal of virology, molecular biology, and disease ecology. Here we provide a detailed analysis of puma lentivirus (PLV) evolution in two natural carnivore hosts, the bobcat and mountain lion. Our results illustrate that PLV evolution is a dynamic process that results from high rates of viral mutation/recombination and host-imposed selection pressure.

  3. Multimodal interaction with BCL-2 family proteins underlies the proapoptotic activity of PUMA BH3.

    Science.gov (United States)

    Edwards, Amanda L; Gavathiotis, Evripidis; LaBelle, James L; Braun, Craig R; Opoku-Nsiah, Kwadwo A; Bird, Gregory H; Walensky, Loren D

    2013-07-25

    PUMA is a proapoptotic BCL-2 family member that drives the apoptotic response to a diversity of cellular insults. Deciphering the spectrum of PUMA interactions that confer its context-dependent proapoptotic properties remains a high priority goal. Here, we report the synthesis of PUMA SAHBs, structurally stabilized PUMA BH3 helices that, in addition to broadly targeting antiapoptotic proteins, directly bind to proapoptotic BAX. NMR, photocrosslinking, and biochemical analyses revealed that PUMA SAHBs engage an α1/α6 trigger site on BAX to initiate its functional activation. We further demonstrated that a cell-permeable PUMA SAHB analog induces apoptosis in neuroblastoma cells and, like expressed PUMA protein, engages BCL-2, MCL-1, and BAX. Thus, we find that PUMA BH3 is a dual antiapoptotic inhibitor and proapoptotic direct activator, and its mimetics may serve as effective pharmacologic triggers of apoptosis in resistant human cancers.

  4. An Environmental Watch System for the Andean countries: El Observatorio Andino

    CERN Document Server

    Muñoz, Ángel G; Velasquez, Ramon; Monterrey, Luis; Leon, Gloria; Ruiz, Franklyn; Recalde, Cristina; Cadena, Jaime; Mejia, Raul; Paredes, Marcos; Bazo, Juan; Reyes, Carmen; Carrasco, Gualberto; Castellon, Yaruska; Villarroel, Claudia; Quintana, Juan; Urdaneta, Avel

    2010-01-01

    An experimental Environmental Watch System, the so-called Observatorio Andino-OA (Observatorio Andino), has been implemented in Venezuela, Colombia, Ecuador, Peru, Bolivia, and Chile over the past two years. The OA is a collaborative and regional network that aims to monitor several environmental variables and develop accurate forecasts based on different scientific tools. Its overall goal is to improve risk assessments, set up Early Warning Systems, support decision-making processes, and provide easily- and intuitively-understandable spatial maps to end-users. The initiative works under the scientific and logistic coordination of the Centro de Modelado Cient\\'ifico (CMC) at Zulia University, Venezuela, and the Centro Internacional para la Investigaci\\'on del Fen\\'omeno 'El Ni\\~no' (CIIFEN), and is operated at a local level by the National Weather Services (NWSs) of the aforementioned six Andean nations. The OA provides several freely-available model outputs including meteorological and hydrological forecasts...

  5. PUMA Development through a Multi physics Approach

    Energy Technology Data Exchange (ETDEWEB)

    Cheon, Jinsik; Kim, Junehyung; Lee, Byoungoon; Lee, Chanbock [Korea Atomic Energy Research Institute, Daejeon (Korea, Republic of)

    2013-05-15

    Meanwhile advances of numerical methods make it possible for the multi physics problem to be solved in a fully coupled way. In addition to a multidimensional, multi physical approach, a nuclear fuel performance analysis code, which is 1D code, should be improved by accommodating the state-of-the-art in the numerical analysis to support current fuel design and performance analysis. In particular, the coupling between the mechanical equilibrium equation and a set of numerically stiff kinetics equations for fission gas release is of great importance for a multi physics simulation of nuclear fuel. Instead, coupling between temperature and fuel constituent was found to be made with a relative ease by employing an ordinary differential equations solver. As an effort for a new SFR metal fuel performance analysis code, called PUMA (Performance of Uranium Metal fuel rod Analysis code), the deformation of U-Zr fuel for SFR in connection with a fission gas release model is analyzed. A finite element analyses for purely mechanical problems are performed using a backward differentiation formula, and are subjected to scrupulous verification with Abaqus. Then mechanical equilibrium equation and the equations for fission gas release are coupled with the same differential-algebraic equations (DAE) solver.

  6. PUMA-mediated mitochondrial apoptotic disruption by hypoxic postconditioning.

    Science.gov (United States)

    Li, YuZhen; Guo, Qi; Liu, XiuHua; Wang, Chen; Song, DanDan

    2015-08-01

    Postconditioning can reduce ischemia-reperfusion (I/R)-induced cardiomyocyte apoptosis by targeting mitochondria. p53 upregulated modulator of apoptosis (PUMA) is involved in lethal I/R injury. Here, we hypothesized that postconditioning might inhibit mitochondrial pathway-mediated cardiomyocyte apoptosis by controlling PUMA expression. The cultured neonatal rat cardiomyocytes underwent 3 h of hypoxia and 3 h of reoxygenation. Postconditioning consisted of three cycles of 5 min reoxygenation and 5 min hypoxia after prolonged hypoxia. Hypoxic postconditioning reduced the levels of PUMA mRNA and protein. Concomitantly, the loss of mitochondrial membrane potential, cytochrome c release and caspase-3 activation were decreased significantly by postconditioning. Overexpression of PUMA increased greatly not only the number of apoptotic cardiomyocytes, but also the collapse of mitochondrial membrane potential, cytochrome c release and caspase-3 activation under postconditioning condition. The data suggest that reduction of PUMA expression mediates the endogenous cardioprotective mechanisms of postconditioning by disrupting mitochondrial apoptotic pathway.

  7. El derecho comunitario andino ¿obstáculo en la integración?

    Directory of Open Access Journals (Sweden)

    Silvana Insignares Cera

    2007-01-01

    Full Text Available El derecho comunitario andino se convierte en un obstáculo para la integración en la medida en que no se aplique adecuadamente el concepto de supranacionalidad y preeminencia frente a las normas internas del respectivo país miembro. De la misma manera, se trata de enfocar el asunto desde la esfera constitucional de los países miembros, poniendo especial énfasis en el caso colombiano

  8. [Identification of individual jaguars (Panthera onca) and pumas (Puma concolor) based on footprint morphometry (Carnivora: Felidae)].

    Science.gov (United States)

    Isasi-Catalá, Emiliana; Barreto, Guillermo R

    2008-12-01

    Estimating feline abundance becomes particularly difficult, sometimes impossible, due to their elusive behavior and extensive space requirements. Available techniques are expensive and/or poorly efficient, therefore alternative methods are needed. The objective of this study was to assess the possibility of identifying individual jaguars and pumas based on morphometric analyses of their tracks. The footprints of five jaguars and four pumas were drawn and the foot (hind or fore foot, left or right foot) and the substrate were recorded. We took 16 measures from each footprint including lengths, widths, areas and angles. Variables were analyzed by using Principal Component Analysis (PCA) and substituted by the first Principal Component (PC) (> 70% variance). We assessed the effect of the substrate and type of foot by means of paired t-student tests, and found differences between fore and hind feet and footprints from the same individual when made on soil or sand. No differences were found between right or left feet. The footprints changed over time as revealed by Multiple ANOVA. Different individuals could be identifyied based on discriminant analyses with more than 70% confidence. We conclude that this method is feasible and can be useful when studying endangered felines.

  9. Evolución tectonomagmática de los Andes bolivianos

    Directory of Open Access Journals (Sweden)

    Néstor Jiménez

    2009-10-01

    Full Text Available Los Andes bolivianos ocuparonuna posición de retroarco durante gran parte del Fanerozoico. En su evoluciónse reconoce una primera etapa, restringida al Paleozoico inferior, en la quehubo una gran transferencia de material sedimentario en la corteza superior, yuna segunda en la que predominó el reciclaje de la masa cortical. A lo largodel Paleozoico inferior, una cuenca marina epicratónica se formó entre elcratón de Amazonia, el macizo Arequipa-Antofalla, y el macizo Pampeanofuncionando inicialmente como cuenca de retroarco y luego como cuenca deantepaís hasta colmatarse en el Paleozoico superior. En este lapso ocurrierontres etapas de deformación: La fase oclóyica (límite Ordovícico-Silúrico decarácter restringido, la fase eohercínica (límite Devónico-Carbonífero, y lafase hercínica (Carbonífero Superior también de influencia areal restringida.En el Mesozoico, se registraron aún breves incursiones marinas antes que en elEoceno comience a edificarse una protocordillera. El solevantamiento general detoda la región centroandina, se inició en el Oligoceno Superior afectando alAltiplano y la Cordillera Oriental actuales. Este solevantamiento ocurrió entres etapas limitadas por la formación de superficies de erosión datadas en 18y 10 Ma. La ladera oeste de la Cordillera Oriental, denominada faja de Huarina,tuvo un rol muy importante en la evolución de la región centroandina. Además decobijar a gran parte del magmatismo de retroarco, en esta faja ocurrió la mayorsubsidencia de la cuenca paleozoica. En esta faja ocurrieron preferentementedurante el Mesozoico, procesos de rifting y de adelgazamiento litosférico. Enel Paleógeno, la faja de Huarinas fue la primera en ser solevantada aislando lacuenca altiplánica del interior del continente, y durante el OligocenoSuperior, se constituyó en el cinturón retrocabalgante del orógeno. Ladeformación en el Altiplano y la Cordillera Oriental concluyó hace 10 Ma cuandose form

  10. Músculos mandibulares de Puma concolor (Mammalia, Carnivora, Felidae

    Directory of Open Access Journals (Sweden)

    Romina Paola Llanos

    2016-09-01

    Full Text Available En este trabajo se describen los músculos mandibulares del puma (Puma concolor, un mamífero carnívoro de amplia distribución en América. Se disecaron y fotografiaron los músculos izquierdos y derechos de la cabeza de tres individuos, dos machos adultos y una hembra juvenil. Este estudio incrementa nuestro conocimiento de los tejidos blandos mandibulares y por lo tanto, aporta información anatómica valiosa de la escasamente conocida y documentada musculatura de este félido, el más grande de la subfamilia Felinae.

  11. Investigation of Adaptive Controllers for Puma Trajectory Tracking

    Science.gov (United States)

    1991-06-01

    Tarokh’s and Seraji’s Control Algorithms - Trajectory ] 4-14 vii 0 Imagen Laser Printer (im132) Owner dsims 00 Host wa7 //printer im132 Date Wed Apr...INVESTIGATION OF ADAPTIVE CONTROLLERS FOR PUMA TRAJECTORY TRACKING THESIS Daniel J. Sims Captain, USAF AFIT/GE/ENG/91J-05 Approved for public release...Adaptive Controllers for PUMA Trajectory Tracking. 6. AUTHOR(S) Daniel J. Sims, Capt, USAF 7. PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES) 8

  12. Comportamientos matrimoniales en migrantes bolivianos residentes en San Salvador de Jujuy : un análisis exploratorio

    Directory of Open Access Journals (Sweden)

    Quintana, Sonia B.

    2005-01-01

    Full Text Available Desde la Biodemografía, los datos relativos a las uniones conyugales son importantes, en razón de su influencia en la estructura genética de las poblaciones. En el estudio de las migraciones de origen boliviano hacia Argentina -desde diferentes disciplinas- se han caracterizado a aquéllas como altamente endogámicas. De allí que nuestro objetivo fue explorar -desde una perspectiva antropológica- las prácticas matrimoniales de bolivianos residentes en la ciudad de San Salvador de Jujuy; para lo cual recurrimos a entrevistas en profundidad realizadas en el marco del proyecto "Los procesos de construcción/reconstrucción de identidades sociales y culturales en espacios fronterizos". Encontramos 46% de uniones exógamas y 54% endógamas (24% constituidas en Argentina y 30 % en Bolivia; situación que no se correspondería con lo reportado para otras ciudades argentinas. Dinámica migratoria en espacios de frontera -como lo es Jujuy-, podría contribuir a la comprensión de lo hasta aquí observado.

  13. Epizootiology and management of feline leukemia virus in the Florida puma.

    Science.gov (United States)

    Cunningham, Mark W; Brown, Meredith A; Shindle, David B; Terrell, Scott P; Hayes, Kathleen A; Ferree, Bambi C; McBride, R T; Blankenship, Emmett L; Jansen, Deborah; Citino, Scott B; Roelke, Melody E; Kiltie, Richard A; Troyer, Jennifer L; O'Brien, Stephen J

    2008-07-01

    Feline leukemia virus (FeLV) was not detected in Florida pumas (Puma concolor coryi) in almost 20 yr of surveillance; however, the finding of two FeLV antigen-positive pumas during the 2002-2003 capture season led to an investigation of FeLV in the population. Between January 1990 and April 2007, the proportion of pumas testing FeLV antibody positive increased, with antibody-positive pumas concentrated in the northern portion of puma range. Five of 131 (4%) pumas sampled between July 2000 and April 2007 were viremic, with all cases clustered in Okaloacoochee Slough (OKS). Clinical signs and clinical pathology at capture were absent or included lymphadenopathy, moderate-to-severe anemia, and lymphopenia. All viremic pumas died; causes of death were septicemia (n=2), intraspecific aggression (n=2), and anemia/dehydration (n=1). Outcome after FeLV exposure in pumas was similar to that in domestic cats, with evidence of regressive, latent, and persistent infections. Management of the epizootic included vaccination, and as of April 2007, 52 free-ranging pumas had received one or more inoculations. Vaccinations were concentrated in OKS and in a band between OKS and the remainder of the puma population. There have been no new cases since July 2004; however, the potential for reintroduction of the virus remains.

  14. Clinical and pathological correlations of marrow PUMA and P53 expressions in myelodysplastic syndromes.

    Science.gov (United States)

    Bektas, Ozlen; Uner, Aysegul; Buyukasik, Yahya; Uz, Burak; Bozkurt, Sureyya; Eliacik, Eylem; Işik, Ayse; Haznedaroglu, Ibrahim Celalettin; Goker, Hakan; Demiroglu, Haluk; Aksu, Salih; Ozcebe, Osman Ilhami; Sayinalp, Nilgun

    2015-05-01

    p53 is a key regulator of apoptosis. p53 upregulated modulator of apoptosis (PUMA) is a critical mediator of p53-dependent and independent apoptosis. The objective of this study was to evaluate the relationship of p53 and PUMA to the prognosis of MDS. Bone marrow biopsies of MDS patients at the time of diagnosis (n = 76) and at the time of transformation (n = 19) were included in the study group. The expression of p53 and PUMA was evaluated using immunohistochemistry. When compared to the control group, both p53 (p PUMA (p = 0.012) expression levels were significantly higher in MDS group. In MDS group, there was a moderate positive correlation between p53 and PUMA expressions. PUMA expression was not correlated with event free and overall survival. However, overall survival was significantly lower in cases with p53 expression in more than 50% of the cells. There was an increase in PUMA expression in cases that showed transformation as compared to the initial diagnostic bone marrows but was not statistically significant. The correlation that existed between p53 and PUMA was lost in transformed cases. Our results showed that PUMA and p53 expressions are increased in MDS marrows compared to normal marrows. PUMA expression increases further during transformation while the expression of p53 is not significantly altered which suggests that PUMA alterations might be a late event during the evolution of MDS.

  15. In vitro binding properties of tumor suppressor p53 with PUMA and NOXA.

    Science.gov (United States)

    Park, So Young; Jeong, Mi Suk; Jang, Se Bok

    2012-04-06

    The p53-upregulated modulator of apoptosis (Puma) and Noxa, are direct targets in p53-mediated apoptosis localized to the mitochondria. Tumor suppressor p53 induces apoptosis by transcriptional induction of Puma and Noxa, which encode proapoptotic BH3-only member Bcl-1 family proteins. However, at a molecular level, the mechanism of action of Puma and Noxa proteins remain poorly defined. In addition, there have been no reports on whether or not p53 directly interacts with Puma and Noxa, in vitro. Here, we provide evidence indicating that the DNA binding domain (DBD) of p53 directly interacted with the BH3 domains of human PUMA and NOXA. Our studies revealed that PUMA has a weak affinity for p53, but NOXA has significant affinity for p53. In this study, we developed a molecular docking model using homology modeling based on the structures of truncated p53, PUMA and NOXA. In addition, we investigated whether or not six mutants of p53 (K101A, T102A, L111A, D186A, G199A and S227A) were able to bind to PUMA and NOXA. Four structure-based mutations (T102A, L111A, D186A and G199A) disrupted the p53-PUMA/NOXA interaction. Our study suggested that these four mutations lowered the stability of the p53 DBD domain and induced aggregation of structurally destabilized p53, and thus disrupted the p53-PUMA/NOXA interaction.

  16. [Food habits of Puma concolor (Carnivora: Felidae) in the Parque Nacional Natural Puracé, Colombia].

    Science.gov (United States)

    Hernández-Guzmán, Andrés; Payán, Esteban; Monroy-Vilchis, Octavio

    2011-09-01

    Neotropical puma (Puma concolor) diet is scarcely known, in particular that of mountain dwelling individuals from Northern South America. This is the first study on pumas from the paramo and the first puma diet analysis for Colombia. The puma diet was studied from 2007 to 2009 in the Puracé National Park in the South Colombian Andes. Paramos are unique neotropical high altitude ecosystems which store and regulate water, and are currently threatened by agricultural expansion and climate change. Seven latrines were monitored for three years and scat collected, washed and dried. Items in scat such as hair, bones, claws and others were separated. Hairs were inspected by microscopy and compared to voucher hair museum specimens. Bone fragments, claws and teeth were also compared to museum collections and identified wherever possible. Additionally, six cameras were set along game trails to document puma and potential prey presence in the area. Food items from five species were identified in 60 puma scats; Northern Pudu (Pudu mephistophiles) was the most important prey in their diet. A total of 354 camera trap-nights photographed a male and female puma, Northern pudu and Spectacled bear (Tremarctos ornatus). The main conclusion suggests a strong dependence of puma on the threatened and mysterious Northern Pudu in paramo habitats. This behavior might reflect restricted prey availability in the high Andes mountains of Colombia, and highlights the plasticity in the puma diet. Conservation actions in the paramo should thus, focus on focal wild species, and in particularly those that show a relationship, such as the one evidenced here with the dependence of puma on Northern Pudu. These findings contribute to increase the little known ecology of Andean puma populations and the species as a whole in Colombia. Baseline data on puma prey populations in different ecosystems throughout their range, is critical to understand the regional requirements for survival, and design

  17. [Knockdown of Puma protects cord blood CD34(+) cells against γ- irradiation].

    Science.gov (United States)

    Zhao, Lei; Zhang, Hong-Yan; Pang, Ya-Kun; Gu, Hai-Hui; Xu, Jing; Yuan, Wei-Ping; Cheng, Tao

    2014-04-01

    Puma (P53 upregulated modulator of apoptosis) is a BCL-2 homology 3 (BH3)-only BCL-1 family member and a critical mediator of P53-dependent and -independent apoptosis. Puma plays an essential role in the apoptosis of hematopoietic stem cells exposed to irradiation without an increased risk of malignancies. This study was purposed to develop an effective lentiviral vector to target Puma in human hematopoietic cells and to investigate the effect of Puma gene knockdown on the biological function of human cord blood CD34(+) cells. SF-LV-shPuma-EGFP and control vectors were constructed, and packaged with the pSPAX2/pMD2.G packaging plasmids via 293T cells to produce pseudo-type lentiviruses. SF-LV-shPuma-EGFP or control lentiviruses were harvested within 72 hours after transfection and then were used to transduce human cord blood CD34(+) cells. GFP(+) transduced cells were sorted by flow cytometry (FCM) for subsequent studies. Semi-quantitative real time RT PCR, Western blot, FCM with Annexin V-PE/7-AAD double staining, Ki67 staining, colony forming cell assay (CFC), CCK-8 assay and BrdU incorporation were performed to determine the expression of Puma and its effect on the cord blood CD34(+) cells. The results showed that Puma was significantly knocked down in cord blood CD34(+) cells and the low expression of Puma conferred a radio-protective effect on the cord blood CD34(+) cells. This effect was achieved through reduced apoptosis and sustained quiescence after irradiation due to Puma knockdown. It is concluded that knockdown of puma gene in CD34(+) hematopoietic stem cells of human cord blood possesses the radioprotective effect, maintains the cells in silence targeting Puma in human hematopoietic cells may have a similar effect with that on mouse hematopoietic cells as previously shown, and our lentiviral targeting system for Puma provides a valuable tool for future translational studies with human cells.

  18. Insectos de los remanentes de bosques secos andinos del norte de Ecuador

    OpenAIRE

    A. Troya; F. Bersosa; Espinoza, L

    2016-01-01

    Las investigaciones biológicas en los bosques secos de Ecuador se centran en aspectos botánicos y ecológicos, mientras que el conocimiento de la entomofauna es casi nulo. Utilizando trampas Malaise , de caída, y golpeteo, se realizaron muestreos en cuatro localidades de bosque seco Andino con el objetivo de identificar la composición de la fauna de insectos terrestres a nivel de familia. Se registraron 14 órdenes y 117 familias, entre las cuales las hormigas (Formicidae) representaron el may...

  19. El poblamiento en el siglo XVI. Contrastes entre el Caribe y el interior andino

    OpenAIRE

    Marta Herrera Ángel

    2007-01-01

    Hablar de poblamiento en el Caribe o en el interior andino y, en general, en América en el siglo XVI, desde la perspectiva de la población que lo habitaba, resulta en buena medida un contrasentido, ya que lo que tuvo lugar en la mayor parte de este continente a lo largo del siglo XVI y buena parte del XVII , como consecuencia de la invasión europea. fue un intenso y continuado proceso de despoblamiento. Ese proceso, en todo caso, no significó la desaparición de la población nativa que, por lo...

  20. Acerca de la transferencia lingüística en el castellano andino

    Directory of Open Access Journals (Sweden)

    Jorge Esquivel Villafana

    2011-07-01

    Full Text Available La coexistencia de códigos lingüísticos de comunicación dentro de unamisma área territorial deviene en un tipo de bilingüismo donde el manejode la segunda lengua testimonia las pautas estructurales que imponela lengua materna. Los términos empleados para caracterizar estefenómeno se han sucedido con mucha o poca suerte, con mayor o menoradecuación empírica. El presente artículo se propone presentar unarevisión de ellos, con especial referencia al concepto de transferencia.Palabras claves: Sintaxis, castellano andino.

  1. Vulnerabilidades urbanas en los países andinos. Introducción general

    OpenAIRE

    D’Ercole, Robert; Hardy, Sébastien; Metzger, Pascale; Robert, Jérémy

    2014-01-01

    1. Vulnerabilidades urbanas Este número temático del Bulletin de l’Institut Français d’Études Andines pretende ser una contribución al conocimiento de la vulnerabilidad urbana de tres países andinos: Bolivia, el Ecuador y el Perú. La vulnerabilidad urbana se entiende como la dificultad de las ciudades para anticipar y enfrentar fenómenos físicos o antrópicos (sismos, inundaciones, deslizamientos de tierra, incendios, explosiones o contaminaciones, etc.) capaces de causar daños, pero también p...

  2. No innovar el orden andino según el licenciado Polo

    OpenAIRE

    Sosa Miatello, Sara

    2014-01-01

    Se analiza la obra del licenciado Juan Polo de Ondegardo, con énfasis en la llamada Relación de 1571 cuya propuesta busca no innovar,en lo posible, el orden andino. Así,sugiere Polo mantener costumbres y leyes preexistentes a la llegada de los españoles, pues solo de ese modo podrían conciliarse los intereses tanto de gobernantes como de gobernados. Por ejemplo, esto es claro en la concepción que tiene dicho personaje del sistema tributario y del régimen de caciques que -mantenidos en esencia...

  3. Diet of pumas (Puma concolor) in Sonora, Mexico, as determined by GPS kill sites and molecular identified scat, with comments on jaguar (Panthera onca) diet

    Science.gov (United States)

    Cassaigne, Ivonne; Medellin, Rodrigo A.; Thompson, Ron W.; Culver, Melanie; Ochoa, Alexander; Vargas, Karla; Childs, Jack L.; Sanderson, Jim; List, Rurik; Torres-Gomez, Armando

    2016-01-01

    We documented puma (Puma concolor) and jaguar (Panthera onca) prey consumption in northeastern Sonora, Mexico, by investigating global positioning system cluster sites (n = 220), and conducting molecular analyses of scat (n = 116) collected between 2011 and 2013. We used camera trap data (n = 8,976 camera days) to estimate relative abundances of pumas and jaguars. Deer (Odocoileus virginianus) was the most frequent prey for puma found at kill sites (67%) and identified from scat (74%), although based on relative numbers of prey consumed, deer represented 45% and lagomorphs 20% of the proportion of all individuals eaten. A variety of small prey (weighing pumas preyed on calves at a higher frequency than previously reported in the same area. In our study area, jaguars preyed on calves at approximately the same rate as pumas (jaguars 3.7 calves per year, pumas 4.9 calves per year). Calculated predation rates were limited only to collared animals within our study area and therefore should not be considered applicable to all pumas and jaguars in Sonora.

  4. Desarrollo y evaluación de un cuestionario semi-cuantitativo de frecuencias alimenticias para adolescentes bolivianos

    Directory of Open Access Journals (Sweden)

    Full Text Available Introducción: Puesto que estudios previos sugieren que los patrones dietéticos y el estilo de vida durante la adolescencia tienen un impacto en la salud adulta, es pertinente monitorear y documentar los patrones alimenticios de los adolescentes. Objetivo: Desarrollar y evaluar la confiabilidad y la validez de un cuestionario semi-cuantitativo de frecuencias alimenticias para adolescentes Bolivianos. Metodología: Una muestra de conveniencia de 82 adolescentes de un colegio secundario de la ciudad de La Paz participaron en el estudio. Un cuestionario semi-cuantitativo de frecuencias alimenticias fue aplicado dos veces con un mes de intervalo (Q1 y Q2. Paralelamente, se obtuvieron tres días de registro dietético (3R efectuados por 38 de los estudiantes, para utilizarlos como patrón referencial. Resultados: La mayoría (87% de las preguntas sobre consumo individual de cada ítem de alimentos presentaron niveles entre módicos a sustanciales de concordancia,11 ítems presentaron un nivel regular mientras que sólo 2 ítems presentaron un nivel ligero de concordancia utilizando la estadística kappa. Las cantidades que se obtuvieron de las dos aplicaciones del cuestionario dieron resultados similares (P > 0,05. Las estimaciones de nutrientes obtenidas de Q1, Q2 y 3R no fueron estadísticamente diferentes (P > 0,05. Conclusión: El cuestionario semi-cuantitativo de frecuencias alimenticias es aparentemente confiable y válido para medir el consumo absoluto de alimentos por adolescentes Bolivianos. Se deberá tener cuidado en el futuro para prevenir sobre-estimaciones por este cuestionario particular.

  5. HER2 Phosphorylates and Destabilizes Pro-Apoptotic PUMA, Leading to Antagonized Apoptosis in Cancer Cells

    OpenAIRE

    Carpenter, Richard L.; Woody Han; Ivy Paw; Hui-Wen Lo

    2013-01-01

    HER2 is overexpressed in 15-20% of breast cancers. HER2 overexpression is known to reduce apoptosis but the underlying mechanisms for this association remain unclear. To elucidate the mechanisms for HER2-mediated survival, we investigated the relationship between HER2 and p53 upregulated modulator of apoptosis (PUMA), a potent apoptosis inducer. Our results showed that HER2 interacts with PUMA, which was independent of HER2 activation. In addition, we observed that HER2 interacted with PUMA i...

  6. PUMA mediates ER stress-induced apoptosis in portal hypertensive gastropathy

    OpenAIRE

    Tan, S; X Wei; Song, M.; Tao, J.; Yang, Y.; Khatoon, S.; Liu, H; Jiang, J.; Wu, B.

    2014-01-01

    Mucosal apoptosis has been demonstrated to be an essential pathological feature in portal hypertensive gastropathy (PHG). p53-upregulated modulator of apoptosis (PUMA) was identified as a BH3-only Bcl-2 family protein that has an essential role in apoptosis induced by a variety of stimuli, including endoplasmic reticulum (ER) stress. However, whether PUMA is involved in mucosal apoptosis in PHG remains unclear, and whether PUMA induces PHG by mediating ER stress remains unknown. The aim of th...

  7. Gli arakuaa iya non ci sono più? Modi di “fare conoscenza” in comunità guaraní del Sud-Est boliviano

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    Silvia Lelli

    2013-07-01

    Full Text Available La situazione della scolarizzazione di quello che attualmente, nel Sud-Est boliviano, si autodefinisce “popolo guaraní” si articola nel rapporto tra la storia della colonizzazione, la costituzione dello Stato boliviano e la storia del movimento indigenista, nelle complesse implicazioni politiche che portano alla configurazione attuale (cfr. Rockwell 1999. Nel percorso tracciato attraverso alcune delle principali problematiche di etnografia della scuola incontrate sul campo emerge lo stretto rapporto tra i contesti locali di “scuola”, “comunità”, “società guaraní” e i macro-contesti “Stato” e “organizzazioni internazionali” implicati nella costruzione del processo educativo (cfr. Geertz 1999.

  8. Jaguar interactions with pumas and prey at the northern edge of jaguars’ range

    Directory of Open Access Journals (Sweden)

    Carmina E. Gutiérrez-González

    2017-01-01

    Full Text Available We present the first study that evaluates jaguar-puma interactions in the arid lands of northern Mexico, where jaguars have their northernmost breeding population and both predators are persecuted for livestock depredation. We tested whether jaguars are the dominant species in this unique ecosystem, where: (1 pumas outnumber jaguars, (2 pumas are better adapted to arid environments, and (3 jaguars and pumas are of similar size. We analyzed four years of data with two approaches; a two species conditional occupancy model and an activity patterns analysis. We used camera location and prey presence as covariates for jaguar and puma detection and presence probabilities. We also explored overlap in activities of predators and prey. Where both species were detected, peccary presence was positively correlated with both jaguar and puma presence, whereas in areas where jaguars were detected but pumas were not, deer presence explained the probability of jaguar presence. We found that both predators were more likely to co-occur together than to be found independently, and so we rejected the hypothesis that jaguars were the dominant species in our study area. Predators were mainly nocturnal and their activity patterns overlapped by 60%. Jaguar, as compared with puma, overlapped more with deer and calves; puma overlapped with calves more than with other prey, suggesting a preference. We believe exploring predator relationships at different scales may help elucidate mechanisms that regulate their coexistence.

  9. Jaguar interactions with pumas and prey at the northern edge of jaguars’ range

    Science.gov (United States)

    2017-01-01

    We present the first study that evaluates jaguar-puma interactions in the arid lands of northern Mexico, where jaguars have their northernmost breeding population and both predators are persecuted for livestock depredation. We tested whether jaguars are the dominant species in this unique ecosystem, where: (1) pumas outnumber jaguars, (2) pumas are better adapted to arid environments, and (3) jaguars and pumas are of similar size. We analyzed four years of data with two approaches; a two species conditional occupancy model and an activity patterns analysis. We used camera location and prey presence as covariates for jaguar and puma detection and presence probabilities. We also explored overlap in activities of predators and prey. Where both species were detected, peccary presence was positively correlated with both jaguar and puma presence, whereas in areas where jaguars were detected but pumas were not, deer presence explained the probability of jaguar presence. We found that both predators were more likely to co-occur together than to be found independently, and so we rejected the hypothesis that jaguars were the dominant species in our study area. Predators were mainly nocturnal and their activity patterns overlapped by 60%. Jaguar, as compared with puma, overlapped more with deer and calves; puma overlapped with calves more than with other prey, suggesting a preference. We believe exploring predator relationships at different scales may help elucidate mechanisms that regulate their coexistence. PMID:28133569

  10. Aβ induces PUMA activation: a new mechanism for Aβ-mediated neuronal apoptosis.

    Science.gov (United States)

    Feng, Jie; Meng, Chengbo; Xing, Da

    2015-02-01

    p53 upregulated modulator of apoptosis (PUMA) is a promising tumor therapy target because it elicits apoptosis and profound sensitivity to radiation and chemotherapy. However, inhibition of PUMA may be beneficial for curbing excessive apoptosis associated with neurodegenerative disorders. Alzheimer's disease (AD) is a representative neurodegenerative disease in which amyloid-β (Aβ) deposition causes neurotoxicity. The regulation of PUMA during Aβ-induced neuronal apoptosis remains poorly understood. Here, we reported that PUMA expression was significantly increased in the hippocampus of transgenic mice models of AD and hippocampal neurons in response to Aβ. PUMA knockdown protected the neurons against Aβ-induced apoptosis. Furthermore, besides p53, PUMA transactivation was also regulated by forkhead box O3a through p53-independent manner following Aβ treatment. Notably, PUMA contributed to neuronal apoptosis through competitive binding of apoptosis repressor with caspase recruitment domain to activate caspase-8 that cleaved Bid into tBid to accelerate Bax mitochondrial translocation, revealing a novel pathway of Bax activation by PUMA to mediate Aβ-induced neuronal apoptosis. Together, we demonstrated that PUMA activation involved in Aβ-induced apoptosis, representing a drug target to antagonize AD progression.

  11. PUMA Cooperates with p21 to Regulate Mammary Epithelial Morphogenesis and Epithelial-To-Mesenchymal Transition.

    Science.gov (United States)

    Zhang, Yanhong; Yan, Wensheng; Jung, Yong Sam; Chen, Xinbin

    2013-01-01

    Lumen formation is essential for mammary morphogenesis and requires proliferative suppression and apoptotic clearance of the inner cells within developing acini. Previously, we showed that knockdown of p53 or p73 leads to aberrant mammary acinus formation accompanied with decreased expression of p53 family targets PUMA and p21, suggesting that PUMA, an inducer of apoptosis, and p21, an inducer of cell cycle arrest, directly regulate mammary morphogenesis. To address this, we generated multiple MCF10A cell lines in which PUMA, p21, or both were stably knocked down. We found that morphogenesis of MCF10A cells was altered modestly by knockdown of either PUMA or p21 alone but markedly by knockdown of both PUMA and p21. Moreover, we found that knockdown of PUMA and p21 leads to loss of E-cadherin expression along with increased expression of epithelial-to-mesenchymal transition (EMT) markers. Interestingly, we found that knockdown of ΔNp73, which antagonizes the ability of wide-type p53 and TA isoform of p73 to regulate PUMA and p21, mitigates the abnormal morphogenesis and EMT induced by knockdown of PUMA or p21. Together, our data suggest that PUMA cooperates with p21 to regulate normal acinus formation and EMT.

  12. PUMA Cooperates with p21 to Regulate Mammary Epithelial Morphogenesis and Epithelial-To-Mesenchymal Transition.

    Directory of Open Access Journals (Sweden)

    Yanhong Zhang

    Full Text Available Lumen formation is essential for mammary morphogenesis and requires proliferative suppression and apoptotic clearance of the inner cells within developing acini. Previously, we showed that knockdown of p53 or p73 leads to aberrant mammary acinus formation accompanied with decreased expression of p53 family targets PUMA and p21, suggesting that PUMA, an inducer of apoptosis, and p21, an inducer of cell cycle arrest, directly regulate mammary morphogenesis. To address this, we generated multiple MCF10A cell lines in which PUMA, p21, or both were stably knocked down. We found that morphogenesis of MCF10A cells was altered modestly by knockdown of either PUMA or p21 alone but markedly by knockdown of both PUMA and p21. Moreover, we found that knockdown of PUMA and p21 leads to loss of E-cadherin expression along with increased expression of epithelial-to-mesenchymal transition (EMT markers. Interestingly, we found that knockdown of ΔNp73, which antagonizes the ability of wide-type p53 and TA isoform of p73 to regulate PUMA and p21, mitigates the abnormal morphogenesis and EMT induced by knockdown of PUMA or p21. Together, our data suggest that PUMA cooperates with p21 to regulate normal acinus formation and EMT.

  13. Capillaria hepatica in Puma concolor: first report in Brazil.

    Science.gov (United States)

    Quadros, Rosiléia M; Pilati, Célso; Marques, Sandra M T; Mazzolli, Marcelo; Benedet, Rodrigo C

    2009-09-01

    Capillaria hepatica was detected by histopathologic diagnosis in two cougars that were shot in April 2008 in Painel, Santa Catarina, Brazil. Macroscopic analysis of their livers revealed the presence of diffuse granulomas, and the histopathologic analysis indicated the presence of C. hepatica eggs, surrounded by mononuclear inflammatory cell infiltrate, small foci of necrosis, and mild-to-moderate fibrosis. This is the first report of C. hepatica in cougars (Puma concolor) in Brazil.

  14. IMPLANTACIÓN DE PRÓTESIS INTRAESCLERAL COMO MANEJO DE GLAUCOMA EN PUMA (PUMA CONCOLOR LINNAEUS, 1758

    Directory of Open Access Journals (Sweden)

    H. Díaz-Parra

    2008-01-01

    Full Text Available Un macho adulto de puma o león americano (Puma concolor, de la Fundación Zoológico santacruz (Colombia, evidenció al examen oftalmológico del ojo derecho buftalmo seve-ro, congestión episcleral, epífora, atrofia retiniana, luxación del cristalino y pérdida de la visión como signos relevantes. debido a los hallazgos del examen clínico y la cronicidad del caso se realizó evisceración e implantación de prótesis intraescleral como parte del tratamiento del glaucoma crónico del animal. después de la cirugía el puma presentó una buena recuperación y adaptación a la prótesis, lo que permitió dar un adecuado manejo de la enfermedad, proporcionando bienestar al animal.

  15. Insectos de los remanentes de bosques secos andinos del norte de Ecuador

    Directory of Open Access Journals (Sweden)

    A. Troya

    2016-01-01

    Full Text Available Las investigaciones biológicas en los bosques secos de Ecuador se centran en aspectos botánicos y ecológicos, mientras que el conocimiento de la entomofauna es casi nulo. Utilizando trampas Malaise , de caída, y golpeteo, se realizaron muestreos en cuatro localidades de bosque seco Andino con el objetivo de identificar la composición de la fauna de insectos terrestres a nivel de familia. Se registraron 14 órdenes y 117 familias, entre las cuales las hormigas (Formicidae representaron el mayor porcentaje de la abundancia total. Se hace referencia a un posible registro de la hormiga Pheidole megacephala , una de las especies invasoras más importantes de mundo. Aunque en forma preliminar, este trabajo constituye el primer aporte al conocimiento de la entomofauna en el ecosistema probablemente más amenazado de este territorio.

  16. COMUNEROS ANDINOS EN LA ADMINISTRACIÓN CHILENA (ARICA, 1880-1929

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    ALBERTO DÍAZ ARAYA

    2011-05-01

    Full Text Available Resumen Se evalúa el impacto generado en los sectores rurales andinos de Arica por la implementación de la estructura y burocracia administrativa chilena post Guerra del Pacífico. A través de registros documentales se hace explícita la participación de comuneros indígenas en la trama estatal chilena en un período de conflicto político y de identidades nacionales. Palabras claves: Comunidad andina – administración chilena – Arica y Tacna. Abstract Assesses the impact generated in the rural Andes Arica for implementing the administrative structure and bureaucracy in Chile after the Pacific War. Through documentary record makes explicit the participation of indigenous community members in the plot Chilean state over a period of political and national identities. Keywords : Andean Community - Chilean government - Arica and Tacna.

  17. El jabalí europeo (Sus scrofa: Un invasor biológico como presa reciente del puma (Puma concolor en el sur de Chile The European wild boar (Sus scrofa: A biological invader as a recent prey of the American puma (Puma concolor in southern Chile

    Directory of Open Access Journals (Sweden)

    OSCAR SKEWES

    2012-06-01

    Full Text Available Se estudió la dieta del puma (Puma concoloren los anos 1988 y 2004 en los faldeos de los volcanes Mocho y Choshuenco, pluviselva valdiviana, sur de Chile, a través de la identificación de ítemes-presas en sus heces y la búsqueda en terreno de carcasas de jabalí europeo (Sus scrofa.Se registra por primera vez al jabalí europeo entre los ítemes-presas del puma en Chile. El puma depredó predominantemente sobre juveniles y los porcentajes de consumo variaron entre un 17-37 % dependiendo del método empleado para analizar el contenido de presas presentes en sus heces.The diet of the American puma (Puma concolorwas studied in 1988 and 2004 in the foothills of the volcanoes Mocho and Choshuenco, Valdivian rainforest, southern Chile, through the identification of prey-items in their feces and field surveys of European wild boar (Sus scrofacarcasses. We reported for the first time the invader European wild boar as a puma's prey in Chile. The puma preys mainly on juveniles and its percentage of consumed prey ranges between 17 and 37 % according to the method employed to assess the analyses of their feces prey contents.

  18. Subcellular localization of PUMA regulates its pro-apoptotic activity in Burkitt's lymphoma B cells.

    Science.gov (United States)

    Ambroise, Gorbatchev; Portier, Alain; Roders, Nathalie; Arnoult, Damien; Vazquez, Aimé

    2015-11-10

    The BH3-only protein PUMA (p53-upregulated modulator of apoptosis) is a major regulator of apoptosis. It belongs to the Bcl-2 family of proteins responsible for maintaining mitochondrial outer membrane integrity by controlling the intrinsic (mitochondrial) apoptotic pathway. We describe here a new pathway regulating PUMA activation through the control of its subcellular distribution. Surprisingly, neither PUMA upregulation in normal activated human B lymphocytes nor high levels of PUMA in Burkitt's lymphoma (BL) were associated with cell death. We show that PUMA is localized to the cytosol in these cells. By contrast, various apoptosis-triggering signals were found to promote the translocation of PUMA to the mitochondria in these cells, leading to their death by apoptosis. This apoptosis was associated with the binding of mitochondrial PUMA to anti-apoptotic members of the Bcl-2 family, such as Bcl-2 and Mcl-1. This translocation was caspase-independent but was prevented by inhibiting or knocking down the expression of the MAPK kinase p38. Our data suggest that the accumulation of PUMA in the cytosol may be important for the participation of this protein in apoptosis without the need for prior transcription. This regulatory pathway may be an important feature of differentiation and tumorigenic processes.

  19. Transnacionalismo andino

    DEFF Research Database (Denmark)

    Pærregaard, Karsten

    2008-01-01

    Formålet med kapitlet er at analyse den transnationale relation mellem to migrantkollektiver i USA og deres oprindelseslandsbyer i Perus højland. Analytisk koncentrerer kapitlet sig om, hvad som motiverer migranterne til at investere tid og penge i deres hjemlandsbyer, og hvordan dette engagement...

  20. Akt Requires Glucose Metabolism to Suppress Puma Expression and Prevent Apoptosis of Leukemic T Cells*

    Science.gov (United States)

    Coloff, Jonathan L.; Mason, Emily F.; Altman, Brian J.; Gerriets, Valerie A.; Liu, Tingyu; Nichols, Amanda N.; Zhao, Yuxing; Wofford, Jessica A.; Jacobs, Sarah R.; Ilkayeva, Olga; Garrison, Sean P.; Zambetti, Gerard P.; Rathmell, Jeffrey C.

    2011-01-01

    The PI3K/Akt pathway is activated in stimulated cells and in many cancers to promote glucose metabolism and prevent cell death. Although inhibition of Akt-mediated cell survival may provide a means to eliminate cancer cells, this survival pathway remains incompletely understood. In particular, unlike anti-apoptotic Bcl-2 family proteins that prevent apoptosis independent of glucose, Akt requires glucose metabolism to inhibit cell death. This glucose dependence may occur in part through metabolic regulation of pro-apoptotic Bcl-2 family proteins. Here, we show that activated Akt relies on glycolysis to inhibit induction of Puma, which was uniquely sensitive to metabolic status among pro-apoptotic Bcl-2 family members and was rapidly up-regulated in glucose-deficient conditions. Importantly, preventing Puma expression was critical for Akt-mediated cell survival, as Puma deficiency protected cells from glucose deprivation and Akt could not readily block Puma-mediated apoptosis. In contrast, the pro-apoptotic Bcl-2 family protein Bim was induced normally even when constitutively active Akt was expressed, yet Akt could provide protection from Bim cytotoxicity. Up-regulation of Puma appeared mediated by decreased availability of mitochondrial metabolites rather than glycolysis itself, as alternative mitochondrial fuels could suppress Puma induction and apoptosis upon glucose deprivation. Metabolic regulation of Puma was mediated through combined p53-dependent transcriptional induction and control of Puma protein stability, with Puma degraded in nutrient-replete conditions and long lived in nutrient deficiency. Together, these data identify a key role for Bcl-2 family proteins in Akt-mediated cell survival that may be critical in normal immunity and in cancer through Akt-dependent stimulation of glycolysis to suppress Puma expression. PMID:21159778

  1. IRES-mediated translation of the pro-apoptotic Bcl2 family member PUMA.

    Science.gov (United States)

    Shaltouki, Atossa; Harford, Terri J; Komar, Anton A; Weyman, Crystal M

    2013-01-01

    The proapoptotic Bcl-2 family member PUMA is a critical regulator of apoptosis. We have previously shown that PUMA plays a pivotal role in the apoptosis associated with skeletal myoblast differentiation and that a MyoD-dependent mechanism is responsible for the increased expression of PUMA in these cells. Herein, we report that the increased expression of PUMA under these conditions involves regulation at the level of translation. Specifically, we have found that the increase in PUMA protein levels occurs under conditions of decreased total protein synthesis, eIF2-alpha phosphorylation and hypophosphorylation of eIF4E-BP, suggesting that PUMA translation is proceeding via an alternative initiation mechanism. Polyribosome analysis of PUMA mRNA further corroborated this suggestion. A combination of in vitro and ex vivo (cellular) approaches has provided evidence suggesting that PUMA mRNA 5'UTR harbors an Internal Ribosome Entry Site (IRES) element. Using mono- and bi-cistronic reporter constructs, we have delineated an mRNA fragment that allows for cap-independent translation in vitro and ex vivo (in skeletal myoblasts) in response to culture in differentiation media (DM), or in response to treatment with the DNA-damaging agent, etoposide. This mRNA fragment also supports translation in HeLa and 293T cells. Thus, our data has revealed a novel IRES-mediated regulation of PUMA expression in several cell types and in response to several stimuli. These findings contribute to our understanding and potential manipulation of any developmental or therapeutic scenario involving PUMA.

  2. PHOTORECEPTOR DEGENERATION IN A MOUNTAIN LION CUB (PUMA CONCOLOR).

    Science.gov (United States)

    DiSalvo, Andrew R; Reilly, Christopher M; Wiggans, K Tomo; Woods, Leslie W; Wack, Ray F; Clifford, Deana L

    2016-12-01

    An orphaned 4-mo-old female mountain lion cub ( Puma concolor ) was captured along the coastline in Montaña de Oro State Park in Los Osos, California, USA. Following suspicion that the cub was visually impaired, ophthalmic examination revealed diffuse bilateral retinal atrophy. Due to a poor prognosis, humane euthanasia was elected. Necropsy and histopathological findings were consistent with photoreceptor degeneration. Based on the cub's signalment, history, and histopathology, a genetic or nutritional etiology was suspected, with the former etiology more strongly supported. To the authors' knowledge, this is the first report of photoreceptor degeneration in a wild felid and should be considered in cases of blindness.

  3. Interfacing models of wildlife habitat and human development to predict the future distribution of puma habitat

    Science.gov (United States)

    Burdett, Christopher L.; Crooks, Kevin R.; Theobald, David M.; Wilson, Kenneth R.; Boydston, Erin E.; Lyren, Lisa A.; Fisher, Robert N.; Vickers, T. Winston; Morrison, Scott A.; Boyce, Walter M.

    2010-01-01

    The impact of human land uses on ecological systems typically differ relative to how extensively natural conditions are modified. Exurban development is intermediate-intensity residential development that often occurs in natural landscapes. Most species-habitat models do not evaluate the effects of such intermediate levels of human development and even fewer predict how future development patterns might affect the amount and configuration of habitat. We addressed these deficiencies by interfacing a habitat model with a spatially-explicit housing-density model to study the effect of human land uses on the habitat of pumas (Puma concolor) in southern California. We studied the response of pumas to natural and anthropogenic features within their home ranges and how mortality risk varied across a gradient of human development. We also used our housing-density model to estimate past and future housing densities and model the distribution of puma habitat in 1970, 2000, and 2030. The natural landscape for pumas in our study area consisted of riparian areas, oak woodlands, and open, conifer forests embedded in a chaparral matrix. Pumas rarely incorporated suburban or urban development into their home ranges, which is consistent with the hypothesis that the behavioral decisions of individuals can be collectively manifested as population-limiting factors at broader spatial scales. Pumas incorporated rural and exurban development into their home ranges, apparently perceiving these areas as modified, rather than non-habitat. Overall, pumas used exurban areas less than expected and showed a neutral response to rural areas. However, individual pumas that selected for or showed a neutral response to exurban areas had a higher risk of mortality than pumas that selected against exurban habitat. Exurban areas are likely hotspots for puma-human conflict in southern California. Approximately 10% of our study area will transform from exurban, rural, or undeveloped areas to suburban or

  4. Cholos, incas y fusionistas: El nuevo Perú y la globalización de lo andino

    OpenAIRE

    Annelou Ypeij

    2013-01-01

    Abstract:   Cholos, incas and fusionistas: The New Peru and the Globalization of 'Lo Andino'This Exploration deals with the cultural changes in Peru from the year 2000. Peru has become a society with more economic, political, social and global opportunities. Within this context, significant cultural changes can be noted. This essay explores cultural expressions such as the weaving associations of the women of Chinchero, the Peruvian cuisine and the rock band Uchpa. All have in common that the...

  5. Cholos, incas y fusionistas: El nuevo Perú y la globalización de lo andino

    Directory of Open Access Journals (Sweden)

    Annelou Ypeij

    2013-04-01

    Full Text Available Abstract:   Cholos, incas and fusionistas: The New Peru and the Globalization of 'Lo Andino'This Exploration deals with the cultural changes in Peru from the year 2000. Peru has become a society with more economic, political, social and global opportunities. Within this context, significant cultural changes can be noted. This essay explores cultural expressions such as the weaving associations of the women of Chinchero, the Peruvian cuisine and the rock band Uchpa. All have in common that they reinterpret 'lo andino', or what it is to be Andean, and that they integrate globalized elements. Based on this, a new identity becomes apparent which surpasses the known categories of ethnicity and class.Resumen:Esta exploración trata sobre los cambios culturales en Perú a partir del año 2000. Perú se ha convertido en una sociedad con más oportunidades económicas, políticas, sociales y globales. En este contexto se pueden notar cambios culturales significantes. El ensayo explora expresiones culturales como las asociaciones de tejidos de las mujeres de Chinchero, la gastronomía peruana y la banda de rock Uchpa. Todas tienen en común que reinterpretan lo andino y que integran elementos globalizados. Basándose en ello, surge una nueva identidad que sobrepasa las conocidas categorías de etnicidad y de clase.

  6. PUMA Internet Task Logging Using the IDAC-1

    Directory of Open Access Journals (Sweden)

    K. N. Tarchanidis

    2014-08-01

    Full Text Available This project uses an IDAC-1 board to sample the joint angle position of the PUMA 76 1 robot and log the results on a computer. The robot is at the task location and the logging computer is located in a different one. The task the robot is performing is based on a Pseudo Stereo Vision System (PSVS. Internet is the transport media. The protocol used in this project is UDP/IP. The actual angle is taken straight from the PUMA controller. High-resolution potentiometers are connected on each robot joint and are buffered and sampled as potential difference on an A/D converter integrated on the IDAC-1. The logging computer through the Internet acting as client asks for the angle set, the IDAC-1 responds as server with the 10-bit resolution sampling of the joint position. The whole task is logged in a file on the logging computer. This application can give the ability to the Internet user to monitor and log the robot tasks anywhere in the Word Wide Web (www.

  7. PUMA promotes Bax translocation in FOXO3a-dependent pathway during STS-induced apoptosis

    Science.gov (United States)

    Zhang, Yingjie; Chen, Qun

    2009-08-01

    PUMA (p53 up-regulated modulator of apoptosis, also called Bbc3) was first identified as a BH3-only Bcl-2 family protein that is transcriptionally up-regulated by p53 and activated upon p53-dependent apoptotic stimuli, such as treatment with DNA-damaging drugs or UV irradiation. Recently studies have been shown that Puma is also up-regulated in response to certain p53-independent apoptotic stimuli, such as growth factor deprivation or treatment with glucocorticoids or STS (staurosporine). However, the molecular mechanisms of PUMA up-regulation and how PUMA functions in response to p53-independent apoptotic stimuli remain poorly understood. In this study, based on real-time single cell analysis, flow cytometry and western blotting technique, we investigated the function of PUMA in living human lung adenocarcinoma cells (ASTC-a-1) after STS treatment. Our results show that FOXO3a was activated by STS stimulation and then translocated from cytosol to nucleus. The expression of PUMA was up-regulated via a FOXO3a-dependent manner after STS treatment, while p53 had little function in this process. Moreover, cell apoptosis and Bax translocation induced by STS were not blocked by Pifithrin-α (p53 inhibitor), which suggested that p53 was not involved in this signaling pathway. Taken together, these results indicate that PUMA promoted Bax translocation in a FOXO3a-dependment pathway during STS-induced apoptosis, while p53 was dispensable in this process.

  8. Slug promotes survival during metastasis through suppression of Puma-mediated apoptosis.

    Science.gov (United States)

    Kim, Seaho; Yao, Jiahong; Suyama, Kimita; Qian, Xia; Qian, Bin-Zhi; Bandyopadhyay, Sanmay; Loudig, Olivier; De Leon-Rodriguez, Carlos; Zhou, Zhen Ni; Segall, Jeffrey; Macian, Fernando; Norton, Larry; Hazan, Rachel B

    2014-07-15

    Tumor cells must overcome apoptosis to survive throughout metastatic dissemination and distal organ colonization. Here, we show in the Polyoma Middle T mammary tumor model that N-cadherin (Cdh2) expression causes Slug (Snai2) upregulation, which in turn promotes carcinoma cell survival. Slug was dramatically upregulated in metastases relative to primary tumors. Consistent with a role in metastasis, Slug knockdown in carcinoma cells suppressed lung colonization by decreasing cell survival at metastatic sites, but had no effect on tumor cell invasion or extravasation. In support of this idea, Slug inhibition by shRNA sensitized tumor cells to apoptosis by DNA damage, resulting in caspase-3 and PARP cleavage. The prosurvival effect of Slug was found to be caused by direct repression of the proapoptotic gene, Puma (Bbc3), by Slug. Consistent with a pivotal role for a Slug-Puma axis in metastasis, inhibition of Puma by RNA interference in Slug-knockdown cells rescued lung colonization, whereas Puma overexpression in control tumor cells suppressed lung metastasis. The survival function of the Slug-Puma axis was confirmed in human breast cancer cells, where Slug knockdown increased Puma expression and inhibited lung colonization. This study demonstrates a pivotal role for Slug in carcinoma cell survival, implying that disruption of the Slug-Puma axis may impinge on the survival of metastatic cells.

  9. Mosaics of Exotic Forest Plantations and Native Forests as Habitat of Pumas

    Science.gov (United States)

    Mazzolli, Marcelo

    2010-08-01

    There is a general lack of information on the impact of forest plantations and the presence of urban settlements on populations of resource-demanding species such as large felids. To partially address this problem, a project study was conducted to find out whether mosaics of forest plantations and native vegetation can function as an adequate habitat for pumas ( Puma concolor) in southern Brazil. The study was conducted within a 1255-km2 area, managed for planted stands of Pinus spp. and Eucalyptus spp. Individual identification of pumas was carried out using a combination of track-matching analysis (discriminant analysis) and camera-trapping. Both techniques recorded closely similar numbers of individual pumas, either total (9-10 individuals) or resident (5-6 individuals). A new approach, developed during this study, was used to individualize pumas by their markings around the muzzle. The estimated density varied from 6.2 to 6.9 individuals/100 km2, ranking among the highest across the entire puma range and indicating a potential total population of up to 87 individuals in the study site. In spite of the availability of extensive areas without human disturbance, a radio-tracked female used a core home range that included forest plantations, an urbanized village, and a two-lane paved road with regular vehicular traffic. The high density of pumas and the species’ intensive use of modified landscapes are interpreted here as deriving from conditions rarely found near human settlements: mutual tolerance by pumas and humans and an adequate habitat (regardless of plantations) largely due to the inhibition of invasions and hunting and maintenance of sizable extents of native forest patches. More widely, it suggests the potential of careful management in forestry operations to provide habitat conditions for resource-demanding species such as the puma. Furthermore, it highlights the importance of curbing invasions and hunting, in this case provided by the presence of

  10. Phosphorylation of Puma modulates its apoptotic function by regulating protein stability

    OpenAIRE

    M. Fricker; O'Prey, J; Tolkovsky, A. M.; Ryan, K.M.

    2010-01-01

    Puma is a potent BH3-only protein that antagonises anti-apoptotic Bcl-2 proteins, promotes Bax/Bak activation and has an essential role in multiple apoptotic models. Puma expression is normally kept very low, but can be induced by several transcription factors including p53, p73, E2F1 and FOXO3a, whereby it can induce an apoptotic response. As Puma can to bind and inactivate all anti-apoptotic members of the Bcl-2 family, its activity must be tightly controlled. We report here, for the first ...

  11. COMPOSICIÓN, ESTRUCTURA Y PATRÓN ESPACIAL DE UN BOSQUE TUCUMANO-BOLIVIANO EN EL DEPARTAMENTO DE TARIJA (BOLIVIA

    Directory of Open Access Journals (Sweden)

    F. Santiago Zenteno-Ruiz

    2010-01-01

    Full Text Available El bosque tucumano-boliviano, en su sector correspondiente a Bolivia, ha comenzado a ser conocido con mayor detalle en los últimos años. Sin embargo, los estudios realizados han tenido un carácter más cualitativo o se han realizado en superficies pequeñas (0,1 ha; además, la biogeografía de sus zonas más bajas no está del todo clara. Por otro lado, algunos aspectos ecológicos, como los referidos a patrones espaciales de los árboles, no han sido nunca abordados. Nosotros realizamos el estudio de una hectárea de bosque tucumano-boliviano a 700-950 m, y mapeamos todos los árboles y lianas con DAP ¿10 cm a fin de caracterizar cuantitativamente un área representativa de este bosque. El propósito fue conocer su composición, abundancia y estructura. Realizamos el primer estudio de patrones espaciales para este tipo de bosque en Bolivia. Registramos 591 individuos agrupados en 31 familias, 47 géneros y 57 especies. Seis especies fueron las más abundantes: Urera caracasana, Trichilia clausenii, Piper tucumanum, Diatenopteryx sorbifolia, Chrysophyllum gonocarpum y Cupania vernalis. El bosque estudiado puede considerarse parte del bosque tucumano-boliviano más que del Arco Pleistocénico. El área basal total fue de 29.4 m2/ha. La presencia de lianas fue relativamente baja, lo que indica que se trata de un bosque relativamente poco perturbado. Se trata de un lugar diverso. La riqueza específica resultó comparable a la de los bosques secos de Madidi (La Paz, Bolivia, y superior a la del tucumano-boliviano en el lado argentino, y fue estimada en 74 especies de árboles. La diversidad (3,5 índice Shannon-Wiener es tan alta como la de muchos bosques más tropicales, e incluso algunos amazónicos. Encontramos un patrón espacial aleatorio en los individuos de la comunidad, y la mayoría de las especies presentó agregación a alguna escala espacial.

  12. Las encuestas: Herramientas necesarias de los estudios socioterritoriales. : Un relato metodológico a partir de encuestas de migraciones desarrolladas en el Sur Boliviano.

    OpenAIRE

    Martin, Ceydric

    2015-01-01

    International audience; Este capítulo tiene como doble objetivo mostrar el interés de las encuestas por cuestionario y cómo producir datos de buena calidad. A partir del ejemplo de encuestas de migraciones, desarrolladas en el sur boliviano, se propone una metodología que permitirá a los estudiantes visualizar todas las etapas de una encuesta: de los prepa¬rativos a los análisis de datos, pasando por el muestreo, el cuestio¬nario (enfoque espacial), la codificación y la carga. Se concluye con...

  13. Estados sin nación. El discurso nacionalista y el evolucionismo andino, 1890-1930

    Directory of Open Access Journals (Sweden)

    Paul Juan Montoya Vásquez

    2008-06-01

    Full Text Available The objective of this essay is to analyze the relationship between the nationalist pronouncements in Ecuador, Peru and Bolivia, and the debate center on European evolutionism at the end of the 19th and 20th centuries. The essay, introductory in nature, presents some aspects of the recent debate on the genealogic discourse of nationalism as well as some important bibliographic references concerning these issues.//El artículo propone un análisis de las relaciones entre el discurso nacionalista en los países centro andinos (Ecuador, Perú, Bolivia y el debate en torno a las tesis del evolucionismo europeo, a fines del siglo XIX e inicios del XX, centrado en el problema de la “integración de las mayorías” a la vida o dinámica nacional. Por ser de carácter introductorio se avoca a presentar algunos elementos del debate reciente sobre el discurso genealógico del nacionalismo así como parte importante de la bibliografía dedicada al tema en los referidos medios.

  14. Mammalian energetics. Instantaneous energetics of puma kills reveal advantage of felid sneak attacks.

    Science.gov (United States)

    Williams, Terrie M; Wolfe, Lisa; Davis, Tracy; Kendall, Traci; Richter, Beau; Wang, Yiwei; Bryce, Caleb; Elkaim, Gabriel Hugh; Wilmers, Christopher C

    2014-10-03

    Pumas (Puma concolor) live in diverse, often rugged, complex habitats. The energy they expend for hunting must account for this complexity but is difficult to measure for this and other large, cryptic carnivores. We developed and deployed a physiological SMART (species movement, acceleration, and radio tracking) collar that used accelerometry to continuously monitor energetics, movements, and behavior of free-ranging pumas. This felid species displayed marked individuality in predatory activities, ranging from low-cost sit-and-wait behaviors to constant movements with energetic costs averaging 2.3 times those predicted for running mammals. Pumas reduce these costs by remaining cryptic and precisely matching maximum pouncing force (overall dynamic body acceleration = 5.3 to 16.1g) to prey size. Such instantaneous energetics help to explain why most felids stalk and pounce, and their analysis represents a powerful approach for accurately forecasting resource demands required for survival by large, mobile predators.

  15. Correlation of Puma airloads: Evaluation of CFD prediction methods

    Science.gov (United States)

    Strawn, Roger C.; Desopper, Andre; Miller, Judith; Jones, Alan

    1989-01-01

    A cooperative program was undertaken by research organizations in England, France, Australia and the U.S. to study the capabilities of computational fluid dynamics codes (CFD) to predict the aerodynamic loading on helicopter rotor blades. The program goal is to compare predictions with experimental data for flight tests of a research Puma helicopter with rectangular and swept tip blades. Two topics are studied. First, computed results from three CFD codes are compared for flight test cases where all three codes use the same partial inflow-angle boundary conditions. Second, one of the CFD codes (FPR) is iteratively coupled with the CAMRAD/JA helicopter performance code. These results are compared with experimental data and with an uncoupled CAMRAD/JA solution. The influence of flow field unsteadiness is found to play an important role in the blade aerodynamics. Alternate boundary conditions are suggested in order to properly model this unsteadiness in the CFD codes.

  16. Correlation of Puma airfoils - Evaluation of CFD prediction methods

    Science.gov (United States)

    Strawn, Roger C.; Desopper, Andre; Miller, Judith; Jones, Alan

    1989-01-01

    A cooperative program was undertaken by research organizations in England, France, Australia and the U.S. to study the capabilities of computational fluid dynamics codes (CFD) to predict the aerodynamic loading on helicopter rotor blades. The program goal is to compare predictions with experimental data for flight tests of a research Puma helicopter with rectangular and swept tip blades. Two topics are studied. First, computed results from three CFD codes are compared for flight test cases where all three codes use the same partial inflow-angle boundary conditions. Second, one of the CFD codes (FPR) is iteratively coupled with the CAMRAD/JA heilcopter performance code. These results are compared with experimental data and with an uncoupled CAMRAD/JA solution. The influence of flow field unsteadiness is found to play an important role in the blade aerodynamics. Alternate boundary conditions are suggested in order to properly model this unsteadiness in the CFD codes.

  17. 波鞋之变异Puma Go Court Laser

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    运动品牌商PUMA近年推出的鞋越来越不像波鞋,淡化了运动的功能性,取而代之的则是更多的潮流设计元素出现在新鞋中,所以,称PUMA此类鞋为“时装波鞋”比“运动波鞋”会更加贴切。处于运动界潮流最尖端的PUMA本季推出的全新“时装鞋”代表作Puma Go Court Laser上市,

  18. Medium-grade astrocytoma in a cougar (Puma concolor).

    Science.gov (United States)

    Kondo, Hirotaka; Leone, Angelique M; Erlacher-Reid, Claire; Gary, Joy; Kiupel, Matti; Farina, Lisa L; Abbott, Jeffrey R

    2012-12-01

    A 17-year-old, male castrated cougar (Puma concolor) was presented minimally responsive and severely depressed, with bilateral mydriasis and absent pupillary light response. On gross examination of the brain, there was a tan-to-gray, invasive mass with a central cavitation on the ventral aspect in the left cerebral hemisphere, rostral to the caudate nucleus. On histopathologic examination, the mass was composed of sheets of medium-sized, round-to-polygonal cells that were multifocally separated by islands of neuropil. Approximately 80% of the neoplastic cells showed strong cytoplasmic labeling for glial fibrillary acidic protein. These findings were consistent with a medium-grade astrocytoma. To the authors' knowledge, neoplastic disease of the central nervous system has not been previously reported in cougars.

  19. Evaluation of the BH3-only protein Puma as a direct Bak activator.

    Science.gov (United States)

    Dai, Haiming; Pang, Yuan-Ping; Ramirez-Alvarado, Marina; Kaufmann, Scott H

    2014-01-03

    Interactions among Bcl-2 family proteins play critical roles in cellular life and death decisions. Previous studies have established the BH3-only proteins Bim, tBid, and Noxa as "direct activators" that are able to directly initiate the oligomerization and activation of Bak and/or Bax. Earlier studies of Puma have yielded equivocal results, with some concluding that it also acts as a direct activator and other studies suggesting that it acts solely as a sensitizer BH3-only protein. In the present study we examined the interaction of Puma BH3 domain or full-length protein with Bak by surface plasmon resonance, assessed Bak oligomerization status by cross-linking followed by immunoblotting, evaluated the ability of the Puma BH3 domain to induce Bak-mediated permeabilization of liposomes and mitochondria, and determined the effect of wild type and mutant Puma on cell viability in a variety of cellular contexts. Results of this analysis demonstrate high affinity (KD = 26 ± 5 nM) binding of the Puma BH3 domain to purified Bak ex vivo, leading to Bak homo-oligomerization and membrane permeabilization. Mutations in Puma that inhibit (L141E/M144E/L148E) or enhance (M144I/A145G) Puma BH3 binding to Bak also produce corresponding alterations in Bak oligomerization, Bak-mediated membrane permeabilization and, in a cellular context, Bak-mediated killing. Collectively, these results provide strong evidence that Puma, like Bim, Noxa, and tBid, is able to act as a direct Bak activator.

  20. PUMA is invovled in ischemia/reperfusion-induced apoptosis of mouse cerebral astrocytes.

    Science.gov (United States)

    Chen, H; Tian, M; Jin, L; Jia, H; Jin, Y

    2015-01-22

    PUMA (p53-upregulated modulator of apoptosis), a BH3-only member of the Bcl-2 protein family, is required for p53-dependent and p53-independent forms of apoptosis. PUMA has been invovled in the onset and progress of several diseases, including cancer, acquired immunodeficiency syndrome, and ischemic brain disease. Although many studies have shown that ischemia and reperfusion (I/R) can induce the apoptosis of astrocytes, the role of PUMA in I/R-mediated apoptosis of cerebral astrocyte apoptosis remains unclear. To mimic in vivo I/R conditions, primary mouse cerebral astrocytes were incubated in a combinational cultural condition of oxygen, glucose, and serum deprivation (OSGD) for 1 h followed by reperfusion (OSGD/R). Cell death determination assays and cell viability assays indicated that OSGD and OSGD/R induce the apoptosis of primary cerebral astrocytes. The expression of PUMA was significantly elevated in primary cerebral astrocytes during OSGD/R. Moreover, targeted down-regulation of PUMA by siRNA transfection significantly decreased the OSGD/R-induced apoptosis of primary cerebral astrocytes. We also found that OSGD and OSGD/R triggered the release of cytochrome c in astrocytes, indicating the dependence on a mitochondrial apoptotic pathway. Reactive oxygen species (ROS) was extremely generated during OSGD and OSGD/R, and the elimination of ROS by treated with N-acetyl-L-cysteine (NAC) remarkably inhibited the expression of PUMA and the apoptosis of primary cerebral astrocytes. The activation of Caspase 3 and Caspase 9 was extremely elevated in primary cerebral astrocytes during OSGD. In addition, we found that knockdown of PUMA led to the depressed expression of Bax, cleaved caspase-9 and caspase-3 during OSGD/R. These results indicate that PUMA is invovled in the apoptosis of cerebral astrocytes upon I/R injury.

  1. Overview and Motivation for the PUMA 2004 and 2005 Campaigns

    Science.gov (United States)

    Toohey, D. W.; Ross, M. N.; Avallone, L. M.; Baccus, S.; Baumgardner, D. G.; Davis, S. M.; Herman, R. L.; Kalnajs, L. E.; Kok, G. L.; Thompson, T. L.; Troy, R. F.

    2005-12-01

    The influence of rocket engine emissions on stratospheric ozone concentrations is less significant than influences due to other industrial emissions. Nevertheless, since rocket engines provide an easily detected and well characterized perturbation to atmospheric composition and particle population, in situ plume measurements allow a unique experimental approach to the understanding of important atmospheric processes including particle microphysics, oxidation chemistry and chemical kinetics, and sub-kilometer scale transport and mixing. As part of the Plume Ultrafast Measurements Acquisition (PUMA) effort to characterize mixing and constituent partitioning in rocket plumes, in situ measurements of H2O vapor, ice water content, CO2, O3, and 0.1 to 100 μm particles were obtained from the NASA WB-57 aircraft during intercepts of the plumes of an Atlas IIAS rocket launched on 19 May 2004 and the Space Shuttle launched on 26 July 2005, both from Cape Canaveral, FL. Data were collected during multiple plume encounters between 14 and 18 km altitude beginning within 10 minutes after launch of each vehicle. This presentation discusses the rationale behind the selection of the suite of instruments, the strategy employed for sampling, and some important first results. Known H2O, CO2, and alumina particle emissions of the rockets' propulsion are used to (1) test our understanding of the role that H2O may play in the activation of alumina particle surfaces, (2) examine potential changes to ice vapor pressure in response to HNO3 production on the ice surfaces, (3) establish consistency of the H2O measurements at various relative humidities in the plume, and (4) characterize the dilution rate of the plume. Additional details and early scientific results will be presented in companion posters by individual PUMA investigators at this session.

  2. Scale dependent behavioral responses to human development by a large predator, the puma.

    Science.gov (United States)

    Wilmers, Christopher C; Wang, Yiwei; Nickel, Barry; Houghtaling, Paul; Shakeri, Yasaman; Allen, Maximilian L; Kermish-Wells, Joe; Yovovich, Veronica; Williams, Terrie

    2013-01-01

    The spatial scale at which organisms respond to human activity can affect both ecological function and conservation planning. Yet little is known regarding the spatial scale at which distinct behaviors related to reproduction and survival are impacted by human interference. Here we provide a novel approach to estimating the spatial scale at which a top predator, the puma (Puma concolor), responds to human development when it is moving, feeding, communicating, and denning. We find that reproductive behaviors (communication and denning) require at least a 4× larger buffer from human development than non-reproductive behaviors (movement and feeding). In addition, pumas give a wider berth to types of human development that provide a more consistent source of human interference (neighborhoods) than they do to those in which human presence is more intermittent (arterial roads with speeds >35 mph). Neighborhoods were a deterrent to pumas regardless of behavior, while arterial roads only deterred pumas when they were communicating and denning. Female pumas were less deterred by human development than males, but they showed larger variation in their responses overall. Our behaviorally explicit approach to modeling animal response to human activity can be used as a novel tool to assess habitat quality, identify wildlife corridors, and mitigate human-wildlife conflict.

  3. PUMA promotes apoptosis of hematopoietic progenitors driving leukemic progression in a mouse model of myelodysplasia.

    Science.gov (United States)

    Guirguis, A A; Slape, C I; Failla, L M; Saw, J; Tremblay, C S; Powell, D R; Rossello, F; Wei, A; Strasser, A; Curtis, D J

    2016-06-01

    Myelodysplastic syndrome (MDS) is characterized by ineffective hematopoiesis with resultant cytopenias. Increased apoptosis and aberrantly functioning progenitors are thought to contribute to this phenotype. As is the case for other malignancies, overcoming apoptosis is believed to be important in progression toward acute myeloid leukemia (AML). Using the NUP98-HOXD13 (NHD13) transgenic mouse model of MDS, we previously reported that overexpression of the anti-apoptotic protein BCL2, blocked apoptosis and improved cytopenias, paradoxically, delaying leukemic progression. To further understand this surprising result, we examined the role of p53 and its pro-apoptotic effectors, PUMA and NOXA in NHD13 mice. The absence of p53 or PUMA but not NOXA reduced apoptosis and expanded the numbers of MDS-repopulating cells. Despite a similar effect on apoptosis and cell numbers, the absence of p53 and PUMA had diametrically opposed effects on progression to AML: absence of p53 accelerated leukemic progression, while absence of PUMA significantly delayed progression. This may be explained in part by differences in cellular responses to DNA damage. The absence of p53 led to higher levels of γ-H2AX (indicative of persistent DNA lesions) while PUMA-deficient NHD13 progenitors resolved DNA lesions in a manner comparable to wild-type cells. These results suggest that targeting PUMA may improve the cytopenias of MDS without a detrimental effect on leukemic progression thus warranting further investigation.

  4. Uncoupling of PUMA Expression and Apoptosis Contributes to Functional Heterogeneity in Renal Cell Carcinoma - Prognostic and Translational Implications.

    Science.gov (United States)

    Zhou, Xiaoguang; Li, Jielin; Marx, Christina; Tolstov, Yanis; Rauch, Geraldine; Herpel, Esther; Macher-Goeppinger, Stephan; Roth, Wilfried; Grüllich, Carsten; Pahernik, Sascha; Hohenfellner, Markus; Duensing, Stefan

    2015-12-01

    Renal cell carcinoma (RCC) is characterized by a profound disruption of proapoptotic signaling networks leading to chemo- and radioresistance. A key mediator of DNA damage-induced apoptosis is the BH3-only protein PUMA. Given its central role in proapoptotic signaling, we analyzed a series of more than 600 precision-annotated primary RCC specimens for PUMA protein expression. We found a reduced expression of PUMA in 22.6% of RCCs analyzed. Unexpectedly, however, PUMA deficiency was not associated with more aggressive tumor characteristic as expected. Instead, a reduced PUMA expression was associated with a lower TNM stage, lower histopathologic grade, and more favorable cancer-specific patient survival. A direct correlation in a separate patient cohort revealed a profound disconnection between PUMA expression and apoptosis as exemplified by the fact that the tumor with the highest level of apoptotic cells was PUMA deficient. In a series of in vitro studies, we corroborated these results and discovered the highest propensity to undergo apoptosis in an RCC cell line with virtually undetectable PUMA expression. At the same time, PUMA expression was not necessarily associated with stronger apoptosis induction, which underscores the striking functional heterogeneity of PUMA expression and apoptosis in RCC. Collectively, our findings suggest that PUMA-independent mechanisms of cell death exist and may play an important role in suppressing malignant progression. They underscore the functional heterogeneity of RCCs and suggest that PUMA expression alone may not be a suitable predictive biomarker. A better understanding of alternative proapoptotic pathways, however, may help to design novel therapeutic strategies for patients with advanced RCC.

  5. La conquista de la mirada. O la construcción del plano andino como discurso representativo de la cosmovisión indígena andina

    OpenAIRE

    Cruder, Gabriela

    1999-01-01

    Este artículo explora la noción de 'plano andino'. Citando a la investigadora colombiana Gladis Daza, se define al 'plano andino' como el "uso de planos secuencias y planos generales que representan la cosmovisión del campesino indigena que interviene en la realización, quien no permite que se le corte el cuerpo con primeros planos y menos los pies porque prefiere mantener todo el tiempo los pies en la tierra. Esto se constituye un ejemplo de cómo las representaciones culturales se imponen so...

  6. Transcription factor Sox4 is required for PUMA-mediated apoptosis induced by histone deacetylase inhibitor, TSA.

    Science.gov (United States)

    Jang, Sang-Min; Kang, Eun-Jin; Kim, Jung-Woong; Kim, Chul-Hong; An, Joo-Hee; Choi, Kyung-Hee

    2013-08-23

    PUMA is a crucial regulator of apoptotic cell death mediated by p53-dependent and p53-independent mechanisms. In many cancer cells, PUMA expression is induced in response to DNA-damaging reagent in a p53-dependent manner. However, few studies have investigated transcription factors that lead to the induction of PUMA expression via p53-independent apoptotic signaling. In this study, we found that the transcription factor Sox4 increased PUMA expression in response to trichostatin A (TSA), a histone deacetylase inhibitor in the p53-null human lung cancer cell line H1299. Ectopic expression of Sox4 led to the induction of PUMA expression at the mRNA and protein levels, and TSA-mediated up-regulation of PUMA transcription was repressed by the knockdown of Sox4. Using luciferase assays and chromatin immunoprecipitation, we also determined that Sox4 recruits p300 on the PUMA promoter region and increases PUMA gene expression in response to TSA treatment. Taken together, these results suggest that Sox4 is required for p53-independent apoptotic cell death mediated by PUMA induction via TSA treatment.

  7. Using Species Distribution Models to Predict Potential Landscape Restoration Effects on Puma Conservation.

    Science.gov (United States)

    Angelieri, Cintia Camila Silva; Adams-Hosking, Christine; Ferraz, Katia Maria Paschoaletto Micchi de Barros; de Souza, Marcelo Pereira; McAlpine, Clive Alexander

    2016-01-01

    A mosaic of intact native and human-modified vegetation use can provide important habitat for top predators such as the puma (Puma concolor), avoiding negative effects on other species and ecological processes due to cascade trophic interactions. This study investigates the effects of restoration scenarios on the puma's habitat suitability in the most developed Brazilian region (São Paulo State). Species Distribution Models incorporating restoration scenarios were developed using the species' occurrence information to (1) map habitat suitability of pumas in São Paulo State, Southeast, Brazil; (2) test the relative contribution of environmental variables ecologically relevant to the species habitat suitability and (3) project the predicted habitat suitability to future native vegetation restoration scenarios. The Maximum Entropy algorithm was used (Test AUC of 0.84 ± 0.0228) based on seven environmental non-correlated variables and non-autocorrelated presence-only records (n = 342). The percentage of native vegetation (positive influence), elevation (positive influence) and density of roads (negative influence) were considered the most important environmental variables to the model. Model projections to restoration scenarios reflected the high positive relationship between pumas and native vegetation. These projections identified new high suitability areas for pumas (probability of presence >0.5) in highly deforested regions. High suitability areas were increased from 5.3% to 8.5% of the total State extension when the landscapes were restored for ≥ the minimum native vegetation cover rule (20%) established by the Brazilian Forest Code in private lands. This study highlights the importance of a landscape planning approach to improve the conservation outlook for pumas and other species, including not only the establishment and management of protected areas, but also the habitat restoration on private lands. Importantly, the results may inform environmental

  8. Acompanhamento do crescimento dental em Puma concolor mantido em cativeiro Accompaniment of the dental growth in Puma concolor kept in captivity

    Directory of Open Access Journals (Sweden)

    João L. Rossi Junior

    2007-05-01

    Full Text Available Objetivou-se neste trabalho fazer levantamento sobre a troca de dentição decídua por permanente, notadamente dos dentes caninos e a prevalência de maloclusão em pumas (Puma concolor manejados nas instituições visitadas no Estado de São Paulo. Para os estudos utilizou-se amostra constituída de 36 pumas, provenientes de 18 instituições mantenedoras de tais espécies em cativeiro no Estado de São Paulo, sendo que três animais com idade de oito meses, irmãos de ninhada, apresentaram retenção dos dentes caninos decíduos e foram acompanha dos por 2 anos e 8 meses. Todos os animais foram examinados, observando se a oclusão estava de acordo com o normal para a espécie. Os dentes foram identificados um a um, examinados diretamente por meio de explorador odontológico. Os animais que apresentaram retenção dos dentes caninos decíduos não foram tratados, pois a maloclusões aparentemente não comprometia a preensão ou mastigação de alimentos, embora apresentassem acúmulo de alimentos ou indutos moles na região dos dentes com espaço interproximal mais reduzido.The development of dentistry is delayed in the preventive internal medicine for wild animals. Some international papers about wild animal dentistry have already been published regarding some species in wild life or kept in captivity in different geographical regions, but not specifically about the great neotropical felines Panthera onca and Puma concolor. The aim of this study was to survey the prevalence of malocclusion in neotropical felines maintained in the state of São Paulo. For the study a sample of 42 jaguars (Panthera onca and 36 pumas (Puma concolor was used, totalizing 78 animals, proceeding from 18 institutions where such species were kept in captivity. All animals were examined if the occlusion was in accordance with the normal for the species. The teeth were identified one by one, examined directly by means of a dental explorer. Malocclusion was present in 47

  9. La autonomía del derecho comunitario andino y su relación con el derecho

    Directory of Open Access Journals (Sweden)

    Carolina Lourdes Rodríguez Aguilera

    2016-08-01

    Full Text Available La propuesta de indagar acerca de la autonomía del derecho comunitario andino, a partir del sistema de solución de controversias de la Comunidad Andina es un tema de mucho interés, que refleja una realidad compleja en el escenario comercial internacional, como son las interacciones que se están dando entre iniciativas regionales y multilaterales en materia comercial, que son objeto de profundos debates. Este trabajo se centrará en el estudio de casos, en un área específica como las salvaguardias. Resumo: A proposta para saber sobre a autonomia do direito comunitário andino, a partir do sistema de solução de controvérsias Comunidade Andina é um assunto de grande interesse, o que reflete uma realidade complexa na arena do comércio internacional, tais interações são dando iniciativas regionais e multilaterais sobre questões comerciais, que são objeto de intenso debate. Este trabalho vai se concentrar em estudos de caso em uma área específica, tais como salvaguardas.

  10. Evolución tectonomagmática de los Andes bolivianos Tectonomagmaticevolution of the Bolivian Andes

    Directory of Open Access Journals (Sweden)

    Néstor Jiménez

    2009-10-01

    Full Text Available Los Andes bolivianos ocuparonuna posición de retroarco durante gran parte del Fanerozoico. En su evoluciónse reconoce una primera etapa, restringida al Paleozoico inferior, en la quehubo una gran transferencia de material sedimentario en la corteza superior, yuna segunda en la que predominó el reciclaje de la masa cortical. A lo largodel Paleozoico inferior, una cuenca marina epicratónica se formó entre elcratón de Amazonia, el macizo Arequipa-Antofalla, y el macizo Pampeanofuncionando inicialmente como cuenca de retroarco y luego como cuenca deantepaís hasta colmatarse en el Paleozoico superior. En este lapso ocurrierontres etapas de deformación: La fase oclóyica (límite Ordovícico-Silúrico decarácter restringido, la fase eohercínica (límite Devónico-Carbonífero, y lafase hercínica (Carbonífero Superior también de influencia areal restringida.En el Mesozoico, se registraron aún breves incursiones marinas antes que en elEoceno comience a edificarse una protocordillera. El solevantamiento general detoda la región centroandina, se inició en el Oligoceno Superior afectando alAltiplano y la Cordillera Oriental actuales. Este solevantamiento ocurrió entres etapas limitadas por la formación de superficies de erosión datadas en 18y 10 Ma. La ladera oeste de la Cordillera Oriental, denominada faja de Huarina,tuvo un rol muy importante en la evolución de la región centroandina. Además decobijar a gran parte del magmatismo de retroarco, en esta faja ocurrió la mayorsubsidencia de la cuenca paleozoica. En esta faja ocurrieron preferentementedurante el Mesozoico, procesos de rifting y de adelgazamiento litosférico. Enel Paleógeno, la faja de Huarinas fue la primera en ser solevantada aislando lacuenca altiplánica del interior del continente, y durante el OligocenoSuperior, se constituyó en el cinturón retrocabalgante del orógeno. Ladeformación en el Altiplano y la Cordillera Oriental concluyó hace 10 Ma cuandose form

  11. Counterpart experimental study of ISP-42 PANDA tests on PUMA facility

    Energy Technology Data Exchange (ETDEWEB)

    Yang, Jun, E-mail: toyangjun@gmail.com [School of Nuclear Engineering, Purdue University, 400 Central Drive, West Lafayette, IN 47907-1290 (United States); Choi, Sung-Won; Lim, Jaehyok; Lee, Doo-Yong; Rassame, Somboon; Hibiki, Takashi; Ishii, Mamoru [School of Nuclear Engineering, Purdue University, 400 Central Drive, West Lafayette, IN 47907-1290 (United States)

    2013-05-15

    Highlights: ► Counterpart tests were performed on two large-scale BWR integral facilities. ► Similarity of post-LOCA system behaviors observed between two tests. ► Passive core and containment cooling systems work as design in both tests. -- Abstract: A counterpart test to the Passive Nachwärmeabfuhr und Druckabbau Test Anlage (Passive Decay Heat Removal and Depressurization Test Facility, PANDA) International Standard Problem (ISP)-42 test was conducted at the Purdue University Multi-Dimensional Integral Test Assembly (PUMA) facility. Aimed to support code validation on a range of light water reactor (LWR) containment issues, the ISP-42 test consists of six sequential phases (Phases A–F) with separately defined initial and boundary conditions, addressing different stages of anticipated accident scenario and system responses. The counterpart test was performed from Phases A to D, which are within the scope of the normal integral tests performed on the PUMA facility. A scaling methodology was developed by using the PANDA facility as prototype and PUMA facility as test model, and an engineering scaling has been applied to the PUMA facility. The counterpart test results indicated that functions of passive safety systems, such as passive containment cooling system (PCCS) start-up, gravity-driven cooling system (GDCS) discharge, PCCS normal operation and overload function were confirmed in both the PANDA and PUMA facilities with qualitative similarities.

  12. Explaining reported puma-related behaviors and behavioral intentions among northern Arizona residents

    Science.gov (United States)

    Mattson, David J.; Ruther, Elizabeth J.

    2012-01-01

    Management of pumas in the American West is typified by conflict among stakeholders plausibly rooted in life experiences and worldviews. We used a mail questionnaire to assess demographics, nature-views, puma-related life experiences and behaviors, and support for puma-related policies among residents of northern Arizona. Data from the questionnaire (n = 693 respondents) were used to model behaviors and support for policies. Compared to models based on nature-views and life experiences, those based on demographics had virtually no support from the data. The Utilitarian/Dominionistic nature-view had the strongest effect of any variable in six of seven models, and was associated with firearms and opposition to policies that would limit killing pumas. The Humanistic/Moralistic nature-view was positively associated with non-lethal behaviors and policies in five models. Gender had the strongest effect of any demographic variable. Compared to demographics alone, our results suggest that worldviews provide a more meaningful explanation of reported human behaviors and behavioral intentions regarding pumas.

  13. Noxa induces apoptosis in oncogene-expressing cells through catch-and-release mechanism operating between Puma and Mcl-1.

    Science.gov (United States)

    Nakajima, Wataru; Tanaka, Nobuyuki

    2011-10-07

    Tumor suppressor p53 induces apoptosis by transcriptional induction of Noxa and Puma, which encode the proapoptotic BH3-only member of the Bcl-2 family proteins. In the p53-mediated tumor surveillance system, p53 induces apoptosis or replicative senescence in oncogene-expressing cells, resulting in elimination of such cells. In this context, we previously found that Noxa and Puma synergistically induce apoptosis. Here, we found the adenovirus oncogene E1A to induce p53-dependently expression of Puma, but not Noxa. The induced Puma associates with antiapoptotic Bcl-2 protein Mcl-1, accompanied by accumulated Mcl-1 protein on mitochondria. Moreover, E1A also reduces expression of the antiapoptotic Bcl-2 protein Bcl-X(L). In contrast, the DNA-damaging agent adriamycin induces Noxa expression in E1A-expressing cells. Interestingly, Mcl-1 knockdown itself induced apoptosis in E1A-expressing MEFs. Furthermore, Noxa displaced Puma's association with Mcl-1, accompanied by Mcl-1 degradation and apoptosis induction by activating mitochondrial apoptotic executers Bax and Bak. These results suggest that p53-induced apoptosis in oncogene-expressing cells is regulated by differential induction and sequential activation of Noxa and Puma. Accumulated Puma by oncogene enhances susceptibility to apoptosis through "catch" in mitochondria by Mcl-1. Subsequently, in response to DNA-damage, Noxa efficiently induces apoptosis by "release" of Puma from Mcl-1.

  14. The BH3-only protein Puma plays an essential role in p53-mediated apoptosis of chronic lymphocytic leukemia cells.

    Science.gov (United States)

    Zhu, Hai-Jia; Liu, Ling; Fan, Lei; Zhang, Li-Na; Fang, Cheng; Zou, Zhi-Jian; Li, Jian-Yong; Xu, Wei

    2013-12-01

    The purpose of this study was to explore the characteristics and functions of BH3-only proteins Puma, Noxa and Bim in the prognosis, therapy and drug resistance of chronic lymphocytic leukemia (CLL). Puma, Noxa and Bim mRNAs were evaluated by real-time quantitative reverse transcriptase-polymerase chain reaction, and correlations between their expression levels and CLL prognostic markers were analyzed. Primary CLL samples were treated in vitro with fludarabine to investigate the role of Puma, Noxa and Bim in the response to chemotherapeutic drugs which act through activation of the p53 pathway. We found that a low expression level of Puma was associated with some markers of poor prognosis. However, the level of Noxa or Bim was not different in patients with CLL with variant clinical features and prognostic factors. Puma expression was up-regulated after fludarabine treatment in primary CLL cells, but there was no significant difference for Noxa and Bim. Up-regulation of Puma occurred only in CLL cells with functional p53. CLL cells with p53 abnormalities were deficient in the activation of Puma by chemotherapeutics. These results suggest that a lack of Puma induction may contribute to the development of resistance to anticancer agents in CLL.

  15. Pazopanib, a novel multi-kinase inhibitor, shows potent antitumor activity in colon cancer through PUMA-mediated apoptosis.

    Science.gov (United States)

    Zhang, Lingling; Wang, Huanan; Li, Wei; Zhong, Juchang; Yu, Rongcheng; Huang, Xinfeng; Wang, Honghui; Tan, Zhikai; Wang, Jiangang; Zhang, Yingjie

    2017-01-10

    Colon cancer is still the third most common cancer which has a high mortality but low five-year survival rate. Novel tyrosine kinase inhibitors (TKI) such as pazopanib become effective antineoplastic agents that show promising clinical activity in a variety of carcinoma, including colon cancer. However, the precise underlying mechanism against tumor is unclear. Here, we demonstrated that pazopanib promoted colon cancer cell apoptosis through inducing PUMA expression. Pazopanib induced p53-independent PUMA activation by inhibiting PI3K/Akt signaling pathway, thereby activating Foxo3a, which subsequently bound to the promoter of PUMA to activate its transcription. After induction, PUMA activated Bax and triggered the intrinsic mitochondrial apoptosis pathway. Furthermore, administration of pazopanib highly suppressed tumor growth in a xenograft model. PUMA deletion in cells and tumors led to resistance of pazopanib, indicating PUMA-mediated pro-apoptotic and anti-tumor effects in vitro and in vivo. Combing pazopanib with some conventional or novel drugs, produced heightened and synergistic antitumor effects that were associated with potentiated PUMA induction via different pathways. Taken together, these results establish a critical role of PUMA in mediating the anticancer effects of pazopanib in colon cancer cells and provide the rationale for clinical evaluation.

  16. Un abordaje comparativo en torno a la incidencia de la tuberculosis en inmigrantes bolivianos de Buenos Aires y São Paulo

    Directory of Open Access Journals (Sweden)

    Alejandro Goldberg

    Full Text Available Este trabajo expone de manera exploratoria y desde una perspectiva sociosanitaria regional, en clave comparativa, algunas dimensiones de análisis en torno a la incidencia de la tuberculosis en inmigrantes bolivianos de las ciudades de Buenos Aires y São Paulo. En ambos casos, se trata de un padecimiento ligado a sus modos de vida y de trabajo precarios en talleres textiles clandestinos. Sumado a lo anterior, la falta de documentación en regla, las dificultades de acceso a la atención sanitaria pública y el sufrimiento social experimentado, potencian la situación de vulnerabilidad social de estas personas; aumentando los riesgos de infectarse y desarrollar la enfermedad.

  17. Expression of Bim, Noxa, and Puma in non-small cell lung cancer

    Directory of Open Access Journals (Sweden)

    Sakakibara-Konishi Jun

    2012-07-01

    Full Text Available Abstract Background The BH3-only members of the Bcl-2 protein family have been proposed to play a key role in the control of apoptosis and in the initiation of the apoptotic pathways. In this study, we evaluated the expression of Bim, Noxa, and Puma in non-small cell lung cancer (NSCLC. Methods A total of 135 surgically resected NSCLCs were immunohistochemically assessed for Bim, Noxa, and Puma expression. The immunoscores were determined, and then its correlation with either the clinicopathological variables or the survival outcomes were analyzed. Results Immunohistochemical reactivity for Bim, Noxa, and Puma was detected in the cytoplasm of the tumor cells. Bim expression was associated with several clinicopathological factors, including sex (p  Conclusions The current analyses demonstrated that Bim expression in the NSCLCs was associated with both squamous cell carcinoma histology and tumor proliferation.

  18. Organic chemistry of cometary dust as derived from PUMA 1 data

    Science.gov (United States)

    Kissel, J.; Krueger, F. R.

    1989-01-01

    Onboard the Halley Fly-By spacecrafts Vega 1, Vega 2, and Giotto were the dust impact mass spectrometers PUMA 1, PUMA 2, and PIA respectively. PUMA 1 was the most sensitive instrument among them. From its data the occurrence of masslines greater than 60 Daltons could be shown to be statistically significant. An analysis of these masslines lead to a scenario, which could explain the masslines as fragment ions from larger molecules which characterize the chemical nature of cometary organic matter as: (1) highly unsaturated hydrocarbons; (2) some of them containing oxygen; (3) less containing nitrogen; and (4) a few containing oxygen and nitrogen as heteroatoms. From the properties of the spectrometer, also some physical parameters of the dust particles could be inferred, such as their density and structure.

  19. BMW Sauber F1 Team 车队专用——BMW X PUMA

    Institute of Scientific and Technical Information of China (English)

    Brian

    2007-01-01

    Puma x F1.第一时间会想起Puma与法拉利车队合作的法拉利系列.还有,Puma更与法拉利签下长期合作协议。可是.最近却杀出个程咬金-BMW Sauber F1 Team车队,说的是Puma与宝马车队联合设计的新系.当中最注目的有这双Kart Cat Ⅱ F1赛车鞋.鞋形延用Puma Cat一贯的流线形设计,除了用上宝马车队专用的宝蓝色外.中底更用上Puma CeⅡ蜂巢缓震系统,算是向法拉利车队示威吧!

  20. PUMAS: The On-line journal of Math and Science Examples for Pre-College Education

    Science.gov (United States)

    Trainer, Melissa G.; Kahn, Ralph A.

    2015-11-01

    PUMAS - “Practical Uses of Math And Science” - is an on-line collection of brief examples showing how math and science topics taught in K-12 classes can be used in interesting settings, including every day life. The examples are written primarily by scientists, engineers, and other content experts having practical experience with the material. They are aimed mainly at classroom teachers to enrich their presentation of math and science topics. The goal of PUMAS is to capture, for the benefit of pre-college education, the flavor of the vast experience that working scientists have with interesting and practical uses of math and science. There are currently over 80 examples in the PUMAS collection, and they are organized by curriculum topics and tagged with relevant grade levels and curriculum topic benchmarks. The published examples cover a wide range of subject matter: from demonstrating why summer is hot, to describing the fluid dynamics of a lava lamp, to calculating the best age to collect Social Security Benefits. The examples are available to all interested parties via the PUMAS web site: http://pumas.nasa.gov/.We invite the community to participate in the PUMAS collection. We seek scientists and scientific thinkers to provide innovative examples of practical uses for teachers to use to enrich the classroom experience, and content experts to participate in peer-review. We also seek teachers to review examples for originality, accuracy of content, clarity of presentation, and grade-level appropriateness. Finally, we encourage teachers to mine this rich repository for real-world examples to demonstrate the value of math in science in everyday life.

  1. Tracking the source of mercury in coastal populations of California Cougars (puma concolor)

    Science.gov (United States)

    Weiss-Penzias, P. S.; Wilmers, C.; Yovovich, V.; Houghtaling, P.; Torregrosa, A.

    2015-12-01

    As part of a project on the cycling of mercury (Hg) from the ocean to fog and deposition to land in coastal California, the whiskers of pumas from coastal and inland populations in California were analyzed for total Hg (HgT). Previous studies have shown that fog water in coastal California contains enhanced concentrations of monomethyl Hg (MMHg) compared to rain water. The likely source of fog MMHg is from evasion and demethylation of dimethyl Hg (DMHg) from coastal ocean upwelling. The California coast receives seasonal inputs of fog drip, and we hypothesized that if fog water deposition of MMHg was making an impact, the observable effects might be seen in high trophic level predators of the terrestrial ecosystem. Puma whiskers from 88 individuals from the Santa Cruz Mountains, a sub-range of the California Coast Range, were obtained and compared with puma whiskers from 12 individuals from the foothills of the Sierra Nevada Mountains. Mean total Hg in puma whiskers from the coastal population is 1.0 ± 1.5 ug Hg / g whisker (ppm), whereas mean HgT from the inland puma population is 0.13 ± 0.09 ppm. The difference between these means is significant to the 95% confidence level. For the coastal puma population, the whiskers from 10 individuals had HgT concentrations > 2.0 ppm and 3 individuals had HgT > 4 ppm, which exceeds the U.S. EPA reference dose for humans (1 ppm) approaches a level of concern found for other large mammals such as polar bears (5 ppm). The study is ongoing and HgT concentrations will be determined in the fur and flesh of deer from the same locations as the puma whiskers, since deer comprise ~95% of the puma diet. Samples of plants that are likely fed upon by deer that span the coastal-inland transect will also be analyzed for HgT. Estimates of fog frequency spatial patterns, derived from weather satellite observations and topographic modeling, will be compared with the HgT content of plant and animal tissue in coastal California to quantify

  2. PUMa - modelling the groundwater flow in Baltic Sedimentary Basin

    Science.gov (United States)

    Kalvane, G.; Marnica, A.; Bethers, U.

    2012-04-01

    In 2009-2012 at University of Latvia and Latvia University of Agriculture project "Establishment of interdisciplinary scientist group and modelling system for groundwater research" is implemented financed by the European Social Fund. The aim of the project is to develop groundwater research in Latvia by establishing interdisciplinary research group and modelling system covering groundwater flow in the Baltic Sedimentary Basin. Researchers from fields like geology, chemistry, mathematical modelling, physics and environmental engineering are involved in the project. The modelling system is used as a platform for addressing scientific problems such as: (1) large-scale groundwater flow in Baltic Sedimentary Basin and impact of human activities on it; (2) the evolution of groundwater flow since the last glaciation and subglacial groundwater recharge; (3) the effects of climate changes on shallow groundwater and interaction of hydrographical network and groundwater; (4) new programming approaches for groundwater modelling. Within the frame of the project most accessible geological information such as description of geological wells, geological maps and results of seismic profiling in Latvia as well as Estonia and Lithuania are collected and integrated into modelling system. For example data form more then 40 thousands wells are directly used to automatically generate the geological structure of the model. Additionally a groundwater sampling campaign is undertaken. Contents of CFC, stabile isotopes of O and H and radiocarbon are the most significant parameters of groundwater that are established in unprecedented scale for Latvia. The most important modelling results will be published in web as a data set. Project number: 2009/0212/1DP/1.1.1.2.0/09/APIA/VIAA/060. Project web-site: www.puma.lu.lv

  3. A Comparative Analysis of Genetic Diversity and Structure in Jaguars (Panthera onca, Pumas (Puma concolor, and Ocelots (Leopardus pardalis in Fragmented Landscapes of a Critical Mesoamerican Linkage Zone.

    Directory of Open Access Journals (Sweden)

    Claudia Wultsch

    Full Text Available With increasing anthropogenic impact and landscape change, terrestrial carnivore populations are becoming more fragmented. Thus, it is crucial to genetically monitor wild carnivores and quantify changes in genetic diversity and gene flow in response to these threats. This study combined the use of scat detector dogs and molecular scatology to conduct the first genetic study on wild populations of multiple Neotropical felids coexisting across a fragmented landscape in Belize, Central America. We analyzed data from 14 polymorphic microsatellite loci in 1053 scat samples collected from wild jaguars (Panthera onca, pumas (Puma concolor, and ocelots (Leopardus pardalis. We assessed levels of genetic diversity, defined potential genetic clusters, and examined gene flow for the three target species on a countrywide scale using a combination of individual- and population-based analyses. Wild felids in Belize showed moderate levels of genetic variation, with jaguars having the lowest diversity estimates (HE = 0.57 ± 0.02; AR = 3.36 ± 0.09, followed by pumas (HE = 0.57 ± 0.08; AR = 4.20 ± 0.16, and ocelots (HE = 0.63 ± 0.03; AR = 4.16 ± 0.08. We observed low to moderate levels of genetic differentiation for all three target species, with jaguars showing the lowest degree of genetic subdivision across the country, followed by ocelots and pumas. Although levels of genetic diversity and gene flow were still fairly high, we detected evidence of fine-scale genetic subdivision, indicating that levels of genetic connectivity for wild felids in Belize are likely to decrease if habitat loss and fragmentation continue at the current rate. Our study demonstrates the value of understanding fine-scale patterns of gene flow in multiple co-occurring felid species of conservation concern, which is vital for wildlife movement corridor planning and prioritizing future conservation and management efforts within human-impacted landscapes.

  4. A Comparative Analysis of Genetic Diversity and Structure in Jaguars (Panthera onca), Pumas (Puma concolor), and Ocelots (Leopardus pardalis) in Fragmented Landscapes of a Critical Mesoamerican Linkage Zone.

    Science.gov (United States)

    Wultsch, Claudia; Waits, Lisette P; Kelly, Marcella J

    2016-01-01

    With increasing anthropogenic impact and landscape change, terrestrial carnivore populations are becoming more fragmented. Thus, it is crucial to genetically monitor wild carnivores and quantify changes in genetic diversity and gene flow in response to these threats. This study combined the use of scat detector dogs and molecular scatology to conduct the first genetic study on wild populations of multiple Neotropical felids coexisting across a fragmented landscape in Belize, Central America. We analyzed data from 14 polymorphic microsatellite loci in 1053 scat samples collected from wild jaguars (Panthera onca), pumas (Puma concolor), and ocelots (Leopardus pardalis). We assessed levels of genetic diversity, defined potential genetic clusters, and examined gene flow for the three target species on a countrywide scale using a combination of individual- and population-based analyses. Wild felids in Belize showed moderate levels of genetic variation, with jaguars having the lowest diversity estimates (HE = 0.57 ± 0.02; AR = 3.36 ± 0.09), followed by pumas (HE = 0.57 ± 0.08; AR = 4.20 ± 0.16), and ocelots (HE = 0.63 ± 0.03; AR = 4.16 ± 0.08). We observed low to moderate levels of genetic differentiation for all three target species, with jaguars showing the lowest degree of genetic subdivision across the country, followed by ocelots and pumas. Although levels of genetic diversity and gene flow were still fairly high, we detected evidence of fine-scale genetic subdivision, indicating that levels of genetic connectivity for wild felids in Belize are likely to decrease if habitat loss and fragmentation continue at the current rate. Our study demonstrates the value of understanding fine-scale patterns of gene flow in multiple co-occurring felid species of conservation concern, which is vital for wildlife movement corridor planning and prioritizing future conservation and management efforts within human-impacted landscapes.

  5. The challenge of monitoring elusive large carnivores: An accurate and cost-effective tool to identify and sex pumas (Puma concolor) from footprints

    Science.gov (United States)

    2017-01-01

    Acquiring reliable data on large felid populations is crucial for effective conservation and management. However, large felids, typically solitary, elusive and nocturnal, are difficult to survey. Tagging and following individuals with VHF or GPS technology is the standard approach, but costs are high and these methodologies can compromise animal welfare. Such limitations can restrict the use of these techniques at population or landscape levels. In this paper we describe a robust technique to identify and sex individual pumas from footprints. We used a standardized image collection protocol to collect a reference database of 535 footprints from 35 captive pumas over 10 facilities; 19 females (300 footprints) and 16 males (235 footprints), ranging in age from 1–20 yrs. Images were processed in JMP data visualization software, generating one hundred and twenty three measurements from each footprint. Data were analyzed using a customized model based on a pairwise trail comparison using robust cross-validated discriminant analysis with a Ward’s clustering method. Classification accuracy was consistently > 90% for individuals, and for the correct classification of footprints within trails, and > 99% for sex classification. The technique has the potential to greatly augment the methods available for studying puma and other elusive felids, and is amenable to both citizen-science and opportunistic/local community data collection efforts, particularly as the data collection protocol is inexpensive and intuitive. PMID:28273159

  6. Noninvasive individual and species identification of jaguars (Panthera onca), pumas (Puma concolor) and ocelots (Leopardus pardalis) in Belize, Central America using cross-species microsatellites and faecal DNA.

    Science.gov (United States)

    Wultsch, Claudia; Waits, Lisette P; Kelly, Marcella J

    2014-11-01

    There is a great need to develop efficient, noninvasive genetic sampling methods to study wild populations of multiple, co-occurring, threatened felids. This is especially important for molecular scatology studies occurring in challenging tropical environments where DNA degrades quickly and the quality of faecal samples varies greatly. We optimized 14 polymorphic microsatellite loci for jaguars (Panthera onca), pumas (Puma concolor) and ocelots (Leopardus pardalis) and assessed their utility for cross-species amplification. Additionally, we tested their reliability for species and individual identification using DNA from faeces of wild felids detected by a scat detector dog across Belize in Central America. All microsatellite loci were successfully amplified in the three target species, were polymorphic with average expected heterozygosities of HE = 0.60 ± 0.18 (SD) for jaguars, HE = 0.65 ± 0.21 (SD) for pumas and HE = 0.70 ± 0.13 (SD) for ocelots and had an overall PCR amplification success of 61%. We used this nuclear DNA primer set to successfully identify species and individuals from 49% of 1053 field-collected scat samples. This set of optimized microsatellite multiplexes represents a powerful tool for future efforts to conduct noninvasive studies on multiple, wild Neotropical felids.

  7. Pro-apoptotic Gene PUMA and Tumor Therapy%促凋亡基因puma与肿瘤治疗

    Institute of Scientific and Technical Information of China (English)

    王明立

    2012-01-01

    p53上调凋亡调控因子(puma)是Bcl-2蛋白家族的促凋亡成员之一,是p53的下游靶基因,发挥作用却与p53的状态无关,可通过p53依赖和非依赖途径诱导细胞凋亡.puma在多种肿瘤组织中低表达,与肿瘤的发生密切相关.puma与放疗、化疗有协同作用,上调puma可以增强肿瘤细胞对放化疗的敏感性,而puma缺失则可降低放化疗对正常细胞的损伤.随着研究的深入,puma有望成为肿瘤治疗的新靶点.%p53 upregulated modulator of apoptosis( PUMA )is a pro-apoptotic protein member of the Bcl-2 family. PUMA is the down-stream target gene of p53, playing the role regardless of the state of p53. PUMA induces apoptosis via both p53-dependent and p53-independent mechanisms. PUMA has low expression in a variety of tumor tissues and is closely related with tumor occurrence. PUMA has a syner-gistic effect with radiotherapy and chemotherapy. Upregulation of PUMA expression could enhance the sensitivity of tumor cells to radiotherapy and chemotherapy, while the deletion of PUMA could reduce the damage of radiotherapy and chemotherapy to normal cells. PUMA is expected to be a new target of cancer therapy along with the further studies.

  8. Identification of a coupled flapping/inflow model for the PUMA helicopter from flight test data

    Science.gov (United States)

    Du Val, Ronald; Bruhis, Ofer; Green, John

    1989-01-01

    A model validation procedure is applied to a coupled flapping/inflow model of a PUMA helicopter blade. The structure of the baseline model is first established. Model structure and flight test data are checked for consistency. Parameters of the model are then identified from the flight test data.

  9. Acute lead toxicosis via ingestion of spent ammunition in a free-ranging cougar (Puma concolor).

    Science.gov (United States)

    Burco, Julia; Myers, Anne Mary; Schuler, Krysten; Gillin, Colin

    2012-01-01

    Lead toxicity has long been documented and acknowledged as a significant health issue of water birds and avian scavengers. However, few instances of toxic effects to higher mammalian carnivores have been documented. Here we present an acute case of lead toxicity in a free-ranging cougar (Puma concolor) in Oregon.

  10. High consumption of primates by pumas and ocelots in a remnant of the Brazilian Atlantic Forest

    Directory of Open Access Journals (Sweden)

    JL Santos

    Full Text Available We studied the diet of the ocelot and puma during the years 2007 and 2008 at the Feliciano Miguel Abdala Reserve, in Minas Gerais, south-eastern Brazil. We collected 49 faecal samples (scats from cats, and identified the species of cat from 23 of them by the analysis of the microstructure patterns of hairs found in their faeces: 17 scats of the puma (Puma concolor and six of the ocelot (Leopardus pardalis. In the puma scats, we identified three species of primates (Brachyteles hypoxanthus, Alouatta guariba and Sapajus nigritus, the remains of which were found in eight of 17 collected (47.1%, representing 26.7% of items consumed. For the ocelot, we detected capuchin monkey (S. nigritus remains in three of the six scats (50%, accounting for 18.7% of items consumed by ocelot. We were unable to identify the cat species in the remaining 26 faecal samples, but we were able to analyse the food items present. Primates were found in five of these 26 faeces (19.2% and represented 10.2% of the items found. Although the sample size is limited, our results indicate a relatively high consumption of primates by felines. We believe that this high predation may be the result of the high local density of primates as well as the greater exposure to the risks of predation in fragmented landscapes, which tends to increase the incidence of the primates using the ground.

  11. Harnessing the p53-PUMA Axis to Overcome DNA Damage Resistance in Renal Cell Carcinoma

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    Xiaoguang Zhou

    2014-12-01

    Full Text Available Resistance to DNA damage–induced apoptosis is a hallmark of cancer and a major cause of treatment failure and lethal disease outcome. A tumor entity that is largely resistant to DNA-damaging therapies including chemo- or radiotherapy is renal cell carcinoma (RCC. This study was designed to explore the underlying molecular mechanisms of DNA damage resistance in RCC to develop strategies to resensitize tumor cells to DNA damage–induced apoptosis. Here, we show that apoptosis-resistant RCC cells have a disconnect between activation of p53 and upregulation of the downstream proapoptotic protein p53 upregulated modulator of apoptosis (PUMA. We demonstrate that this disconnect is not caused by gene-specific repression through CCCTC-binding factor (CTCF but instead by aberrant chromatin compaction. Treatment with an HDAC inhibitor was found to effectively reactivate PUMA expression on the mRNA and protein level and to revert resistance to DNA damage–induced cell death. Ectopic expression of PUMA was found to resensitize a panel of RCC cell lines to four different DNA-damaging agents tested. Remarkably, all RCC cell lines analyzed were wild-type for p53, and a knockdown was likewise able to sensitize RCC cells to acute genotoxic stress. Taken together, our results indicate that DNA damage resistance in RCC is reversible, involves the p53-PUMA axis, and is potentially targetable to improve the oncological outcomes of RCC patients.

  12. Three pathogens in sympatric populations of pumas, bobcats, and domestic cats: implications for infections disease transmission

    Science.gov (United States)

    Bevins, Sarah N.; Carver, Scott; Boydston, Erin E.; Lyren, Lisa M.; Alldredge, Mat; Logan, Kenneth A.; Riley, Seth P.D.; Fisher, Robert N.; Vickers, T. Winston; Boyce, Walter; Salman, Mo; Lappin, Michael R.; Crooks, Kevin R.; VandeWoude, Sue

    2012-01-01

    Anthropogenic landscape change can lead to increased opportunities for pathogen transmission between domestic and non-domestic animals. Pumas, bobcats, and domestic cats are sympatric in many areas of North America and share many of the same pathogens, some of which are zoonotic. We analyzed bobcat, puma, and feral domestic cat samples collected from targeted geographic areas. We examined exposure to three pathogens that are taxonomically diverse (bacterial, protozoal, viral), that incorporate multiple transmission strategies (vector-borne, environmental exposure/ingestion, and direct contact), and that vary in species-specificity. Bartonella spp., Feline Immunodeficiency Virus (FIV), and Toxoplasma gondii IgG were detected in all three species with mean respective prevalence as follows: puma 16%, 41% and 75%; bobcat 31%, 22% and 43%; domestic cat 45%, 10% and 1%. Bartonella spp. were highly prevalent among domestic cats in Southern California compared to other cohort groups. Feline Immunodeficiency Virus exposure was primarily associated with species and age, and was not influenced by geographic location. Pumas were more likely to be infected with FIV than bobcats, with domestic cats having the lowest infection rate. Toxoplasma gondii seroprevalence was high in both pumas and bobcats across all sites; in contrast, few domestic cats were seropositive, despite the fact that feral, free ranging domestic cats were targeted in this study. Interestingly, a directly transmitted species-specific disease (FIV) was not associated with geographic location, while exposure to indirectly transmitted diseases - vectorborne for Bartonella spp. and ingestion of oocysts via infected prey or environmental exposure for T. gondii - varied significantly by site. Pathogens transmitted by direct contact may be more dependent upon individual behaviors and intra-specific encounters. Future studies will integrate host density, as well as landscape features, to better understand the

  13. Three pathogens in sympatric populations of pumas, bobcats, and domestic cats: Implications for infectious disease transmission

    Science.gov (United States)

    Bevins, S.N.; Carver, S.; Boydston, E.E.; Lyren, L.M.; Alldredge, M.; Logan, K.A.; Riley, S.P.D.; Fisher, R.N.; Vickers, T.W.; Boyce, W.; Salman, M.; Lappin, M.R.; Crooks, K.R.; VandeWoude, S.

    2012-01-01

    Anthropogenic landscape change can lead to increased opportunities for pathogen transmission between domestic and non-domestic animals. Pumas, bobcats, and domestic cats are sympatric in many areas of North America and share many of the same pathogens, some of which are zoonotic. We analyzed bobcat, puma, and feral domestic cat samples collected from targeted geographic areas. We examined exposure to three pathogens that are taxonomically diverse (bacterial, protozoal, viral), that incorporate multiple transmission strategies (vector-borne, environmental exposure/ingestion, and direct contact), and that vary in species-specificity. Bartonella spp., Feline Immunodeficiency Virus (FIV), and Toxoplasma gondii IgG were detected in all three species with mean respective prevalence as follows: puma 16%, 41% and 75%; bobcat 31%, 22% and 43%; domestic cat 45%, 10% and 1%. Bartonella spp. were highly prevalent among domestic cats in Southern California compared to other cohort groups. Feline Immunodeficiency Virus exposure was primarily associated with species and age, and was not influenced by geographic location. Pumas were more likely to be infected with FIV than bobcats, with domestic cats having the lowest infection rate. Toxoplasma gondii seroprevalence was high in both pumas and bobcats across all sites; in contrast, few domestic cats were seropositive, despite the fact that feral, free ranging domestic cats were targeted in this study. Interestingly, a directly transmitted species-specific disease (FIV) was not associated with geographic location, while exposure to indirectly transmitted diseases - vector-borne for Bartonella spp. and ingestion of oocysts via infected prey or environmental exposure for T. gondii - varied significantly by site. Pathogens transmitted by direct contact may be more dependent upon individual behaviors and intra-specific encounters. Future studies will integrate host density, as well as landscape features, to better understand the

  14. Three pathogens in sympatric populations of pumas, bobcats, and domestic cats: implications for infectious disease transmission.

    Directory of Open Access Journals (Sweden)

    Sarah N Bevins

    Full Text Available Anthropogenic landscape change can lead to increased opportunities for pathogen transmission between domestic and non-domestic animals. Pumas, bobcats, and domestic cats are sympatric in many areas of North America and share many of the same pathogens, some of which are zoonotic. We analyzed bobcat, puma, and feral domestic cat samples collected from targeted geographic areas. We examined exposure to three pathogens that are taxonomically diverse (bacterial, protozoal, viral, that incorporate multiple transmission strategies (vector-borne, environmental exposure/ingestion, and direct contact, and that vary in species-specificity. Bartonella spp., Feline Immunodeficiency Virus (FIV, and Toxoplasma gondii IgG were detected in all three species with mean respective prevalence as follows: puma 16%, 41% and 75%; bobcat 31%, 22% and 43%; domestic cat 45%, 10% and 1%. Bartonella spp. were highly prevalent among domestic cats in Southern California compared to other cohort groups. Feline Immunodeficiency Virus exposure was primarily associated with species and age, and was not influenced by geographic location. Pumas were more likely to be infected with FIV than bobcats, with domestic cats having the lowest infection rate. Toxoplasma gondii seroprevalence was high in both pumas and bobcats across all sites; in contrast, few domestic cats were seropositive, despite the fact that feral, free ranging domestic cats were targeted in this study. Interestingly, a directly transmitted species-specific disease (FIV was not associated with geographic location, while exposure to indirectly transmitted diseases--vector-borne for Bartonella spp. and ingestion of oocysts via infected prey or environmental exposure for T. gondii--varied significantly by site. Pathogens transmitted by direct contact may be more dependent upon individual behaviors and intra-specific encounters. Future studies will integrate host density, as well as landscape features, to better

  15. Interdependence of Bad and Puma during ionizing-radiation-induced apoptosis.

    Science.gov (United States)

    Toruno, Cristhian; Carbonneau, Seth; Stewart, Rodney A; Jette, Cicely

    2014-01-01

    Ionizing radiation (IR)-induced DNA double-strand breaks trigger an extensive cellular signaling response that involves the coordination of hundreds of proteins to regulate DNA repair, cell cycle arrest and apoptotic pathways. The cellular outcome often depends on the level of DNA damage as well as the particular cell type. Proliferating zebrafish embryonic neurons are highly sensitive to IR-induced apoptosis, and both p53 and its transcriptional target puma are essential mediators of the response. The BH3-only protein Puma has previously been reported to activate mitochondrial apoptosis through direct interaction with the pro-apoptotic Bcl-2 family proteins Bax and Bak, thus constituting the role of an "activator" BH3-only protein. This distinguishes it from BH3-only proteins like Bad that are thought to indirectly promote apoptosis through binding to anti-apoptotic Bcl-2 family members, thereby preventing the sequestration of activator BH3-only proteins and allowing them to directly interact with and activate Bax and Bak. We have shown previously that overexpression of the BH3-only protein Bad in zebrafish embryos supports normal embryonic development but greatly sensitizes developing neurons to IR-induced apoptosis. While Bad has previously been shown to play only a minor role in promoting IR-induced apoptosis of T cells in mice, we demonstrate that Bad is essential for robust IR-induced apoptosis in zebrafish embryonic neural tissue. Moreover, we found that both p53 and Puma are required for Bad-mediated radiosensitization in vivo. Our findings show the existence of a hierarchical interdependence between Bad and Puma whereby Bad functions as an essential sensitizer and Puma as an essential activator of IR-induced mitochondrial apoptosis specifically in embryonic neural tissue.

  16. Interdependence of Bad and Puma during ionizing-radiation-induced apoptosis.

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    Cristhian Toruno

    Full Text Available Ionizing radiation (IR-induced DNA double-strand breaks trigger an extensive cellular signaling response that involves the coordination of hundreds of proteins to regulate DNA repair, cell cycle arrest and apoptotic pathways. The cellular outcome often depends on the level of DNA damage as well as the particular cell type. Proliferating zebrafish embryonic neurons are highly sensitive to IR-induced apoptosis, and both p53 and its transcriptional target puma are essential mediators of the response. The BH3-only protein Puma has previously been reported to activate mitochondrial apoptosis through direct interaction with the pro-apoptotic Bcl-2 family proteins Bax and Bak, thus constituting the role of an "activator" BH3-only protein. This distinguishes it from BH3-only proteins like Bad that are thought to indirectly promote apoptosis through binding to anti-apoptotic Bcl-2 family members, thereby preventing the sequestration of activator BH3-only proteins and allowing them to directly interact with and activate Bax and Bak. We have shown previously that overexpression of the BH3-only protein Bad in zebrafish embryos supports normal embryonic development but greatly sensitizes developing neurons to IR-induced apoptosis. While Bad has previously been shown to play only a minor role in promoting IR-induced apoptosis of T cells in mice, we demonstrate that Bad is essential for robust IR-induced apoptosis in zebrafish embryonic neural tissue. Moreover, we found that both p53 and Puma are required for Bad-mediated radiosensitization in vivo. Our findings show the existence of a hierarchical interdependence between Bad and Puma whereby Bad functions as an essential sensitizer and Puma as an essential activator of IR-induced mitochondrial apoptosis specifically in embryonic neural tissue.

  17. Globalización, nuevas dinámicas urbanas y estrategias de integración de migrantes andinos indocumentados en Suiza

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    Angela Stienen

    2003-01-01

    integración de migrantes indocumentados. En este estudio de caso sobre migrantes indocumentados de países andinos, se discuten los diferentes patrones de su integración social y económica en Suiza, relacionándolos con la aparición de territorios urbanos contestatarios durante los años 80 y el auge de una nueva clase media urbana durante los 90.

  18. Cisplatin induced apoptosis of ovarian cancer A2780s cells by activation of ERK/p53/PUMA signals.

    Science.gov (United States)

    Song, Hao; Wei, Mei; Liu, Wenfen; Shen, Shulin; Li, Jiaqun; Wang, Liming

    2017-03-13

    Cisplatin (CDDP) is one of the most effective anticancer agents widely used in the treatment of solid tumors, including ovarian cancer. It is generally considered as a cytotoxic drug which kills cancer cells by causing DNA damage, and subsequently inducing apoptosis in cancer cells. However, the underlying mechanisms leading to cell apoptosis remain obscure. In this study, the signaling pathways involved in CDDP -induced apoptosis were examined using CDDP-sensitive ovarian cancer A2780s cells. A2780s cells were treated with CDDP (1.5-3 μg/ml) for 6 h, 12 h and 24 h. Using siRNA targeting P53 and PUMA, and a selective MEK inhibitor, PD98059 to examine the relation between ERK1/2 activation, p53 and PUMA expression after exposure to CDDP, and the effect on CDDP-induced apoptosis. The results shown that treatment of A2780s cells with CDDP (3 μg/ml) for 6-24 h induced apoptosis, resulting in the activation of extracellular signal-regulated kinase 1/2 (ERK1/2) and accumulation of p53 and PUMA (p53 upregulated modulator of apoptosis) protein. Knockdown of P53 or PUMA by siRNA transfection blocked CDDP-induced apoptosis. Inhibition of ERK1/2 using PD98059, a selective MEK inhibitor, blocked the apoptotic cell death but prevented CDDP-induced accumulation of p53 and PUMA. Knockdown of P53 by siRNA transfection also blocked CDDP-induced accumulation of PUMA. We therefore concluded that CDDP activated ERK1/2 and induced-p53-dependent PUMA upregulation, resulting in triggering apoptosis in A2780s cells. Our study clearly demonstrates that the ERK1/2/p53/PUMA axis is related to CDDP-induced cell death in A2780s cells.

  19. Role of BH3-only molecules Bim and Puma in β-cell death in Pdx1 deficiency.

    Science.gov (United States)

    Ren, Decheng; Sun, Juan; Wang, Changzheng; Ye, Honggang; Mao, Liqun; Cheng, Emily H; Bell, Graeme I; Polonsky, Kenneth S

    2014-08-01

    Mutations in pancreatic duodenal homeobox-1 (PDX1) are associated with diabetes in humans. Pdx1-haploinsufficient mice develop diabetes due to an increase in β-cell death leading to reduced β-cell mass. For definition of the molecular link between Pdx1 deficiency and β-cell death, Pdx1-haploinsufficient mice in which the genes for the BH3-only molecules Bim and Puma had been ablated were studied on a high-fat diet. Compared with Pdx1(+/-) mice, animals haploinsufficient for both Pdx1 and Bim or Puma genes showed improved glucose tolerance, enhanced β-cell mass, and reduction in the number of TUNEL-positive cells in islets. These results suggest that Bim and Puma ablation improves β-cell survival in Pdx1(+/-) mice. For exploration of the mechanisms responsible for these findings, Pdx1 gene expression was knocked down in mouse MIN6 insulinoma cells resulting in apoptotic cell death that was found to be associated with increased expression of BH3-only molecules Bim and Puma. If the upregulation of Bim and Puma that occurs during Pdx1 suppression was prevented, apoptotic β-cell death was reduced in vitro. These results suggest that Bim and Puma play an important role in β-cell apoptosis in Pdx1-deficient diabetes.

  20. EZH2-mediated Puma gene repression regulates non-small cell lung cancer cell proliferation and cisplatin-induced apoptosis.

    Science.gov (United States)

    Liu, Haidan; Li, Wei; Yu, Xinfang; Gao, Feng; Duan, Zhi; Ma, Xiaolong; Tan, Shiming; Yuan, Yunchang; Liu, Lijun; Wang, Jian; Zhou, Xinmin; Yang, Yifeng

    2016-08-30

    Polycomb group (PcG) proteins are highly conserved epigenetic effectors that maintain the silenced state of genes. EZH2 is the catalytic core and one of the most important components of the polycomb repressive complex 2 (PRC2). In non-small cell lung cancer (NSCLC) cells and primary lung tumors, we found that PRC2 components, including EZH2, are overexpressed. High levels of EZH2 protein were associated with worse overall survival rate in NSCLC patients. RNA interference mediated attenuation of EZH2 expression blunted the malignant phenotype in this setting, exerting inhibitory effects on cell proliferation, anchorage-independent growth, and tumor development in a xenograft mouse model. Unexpectedly, we discovered that, in the suppression of EZH2, p53 upregulated modulator of apoptosis (PUMA) expression was concomitantly induced. This is achieved through EZH2 directly binds to the Puma promoter thus epigenetic repression of PUMA expression. Furthermore, cisplatin-induced apoptosis of EZH2-knocking down NSCLC cells was elevated as a consequence of increased PUMA expression. Our work reveals a novel epigenetic regulatory mechanism controlling PUMA expression and suggests that EZH2 offers a candidate molecular target for NSCLC therapy and EZH2-regulated PUMA induction would synergistically increase the sensitivity to platinum agents in non-small cell lung cancers.

  1. La necesidad de comprometer la existencia. Nación y descolonización en el cine boliviano de la época neoliberal

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    Diego Mattos Vazualdo

    2011-01-01

    Full Text Available The aim of this essay is twofold: to analyze post-dictatorial Bolivian filmic discourse on the thematic of the nation vis-à-vis processes of decolonization, and to observe the effect that this relation had in the formation of subjectivities during the so called  “neoliberal” era . Toward this end, I examine three films that reflect on the colonial condition of the nation through cinematographic language: Mi socio (Paolo Agazzi, 1982, La nación clandestina (Jorge Sanjinés, 1989, and American Visa (Juan Carlos Valdivia, 2005. El objetivo principal del presente ensayo es analizar la reflexión que se da en el discurso fílmico boliviano post-dictatorial sobre la temática de la nación en relación a procesos de descolonización; al mismo tiempo, observar el efecto que dicha relación tuvo en el proceso de formación de subjetividades durante la nueva época denominada “neoliberal”. Para ello, acudo a la lectura de tres películas que de distintas maneras reflexionan la condición colonial de la nación a través del lenguaje cinematográfico: Mi socio (Paolo Agazzi, 1982, La nación clandestina (Jorge Sanjinés, 1989, y American Visa (Juan Carlos Valdivia, 2005.

  2. La reconfiguración del Estado boliviano hacia el plurinacionalismo: economía política de autonomía indígena

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    Nam Kwon Mun

    2014-01-01

    Full Text Available El salto de la sociedad multicultural al Estado plurinacional significa establecer una nueva estructura de poder con base étnica, reconociendo a los grupos étnicos como las naciones y después implementando su estatalización que supone formar parte de las estructuras del Estado. Este trabajo se propone evaluar el alcance y el límite del plurinacionalismo boliviano en la época del gobierno izquierdista, mediante el análisis de la economía política de la Autonomía Indígena Originario Campesina (AIOC, que es la forma más explícita y clara de hacer efectiva la noción de Estado plurinacional. Al fin, la realidad de la AIOC demuestra que el Estado plurinacional encarnado en descentralización ha probado ser sólo retórica vacía de los intentos reformistas.

  3. PUMA gene transfection can enhance the sensitivity of epirubicin-induced apoptosis of MCF-7 breast cancer cells.

    Science.gov (United States)

    Sun, C-G; Zhuang, J; Teng, W-J; Wang, Z; Du, S-S

    2015-05-29

    We explored whether p53 upregulated modulator of apoptosis (PUMA) gene transfection could enhance the sensitivity of epirubicin-induced apoptosis of MCF-7 breast cancer cells. The liposome-mediated recombinant eukaryotic expression vector PU-MA-pCDNA3 and empty vector plasmid were stably transfected into MCF-7 cells. Epirubicin (0.01-100 μM) was applied to MCF-7, MCF-7/PUMA, and MCF-7/pCDNA3 cells for 72 h. The MTT assay was used to calculate the cell survival rate in each group, and the 50% inhibitory concentration (IC50) was calculated. The IC50 values of epirubicin in MCF-7, MCF-7/PUMA, and MCF-7/pCDNA3 cells were 13 ± 1.4, 1.8 ± 0.2, and 10.7 ± 1.3 μM, respectively. The sensitivity of MCF-7/PUMA cells to epirubicin increased 7.2-fold. Epirubicin induced apoptosis in MCF-7 cells dose-dependently, but MCF-7/PUMA cell-induced apoptosis was more significant compared to controls. Low concentrations of epirubicin (0.1 μM) caused low levels of apoptosis of MCF-7/pCDNA3 (1.15 ± 0.26%) and MCF-7 cells (0.9 ± 0.24%), but significantly induced apoptosis of MCF-7/PUMA cells (6.44 ± 1.46%). High epirubicin concentration (1 μM) induced apoptosis in each group, but the epirubicin MCF-7/PUMA apoptosis rate (35.47 ± 9.36%) was significantly higher than that of MCF-7 (12.6 ± 3.73%) and MCF-7/ pCDNA3 (15.2 ± 5.17%) cells (P PUMA protein expression in MCF-7/PUMA cells was significantly higher than that in MCF-7 and MCF-7/pCDNA3 cells by Western blot analysis. There-fore, stable transfection of PUMA can significantly enhance epirubicin-induced apoptosis sensitivity of MCF-7 breast cancer cells.

  4. Análisis de la industria cultural en el pacto de integración sub-regional andino

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    Nilo Fernández

    2004-01-01

    Full Text Available El objetivo general de la presente investigacion fue realizar un analisis de la industria cultural en el pacto de la integracion Sub-regional Andino, destancado el papel fundamental de esta industria como entidad de comunicacion que se construye y se modela a travez de signos culturales que se encuentran en la cotidianidad, en el discurso social y surgidas de realidades entrelazadas, siendo los medios de comunicacion los que hacen percibirlo de otra manera. Las bases teoricas describen como algunos elementos tento del contexto multidimensional de la region andina como elemento socio-juridicos y culturales influyen los aportes de autores tales como: Lorena Rincon, Lucrecia Morales, German Rey, Bernardo Klisberg, Marcelino Bisbal y Nestor Garcia Canclini, entre otros. El tipo de investigacion que se selecciono fue la descriptiva documental; para la cual se utilizo como instrumento de analisis una guia de observacion que permitio ubicar las principales categoria, sub-categorias y las unidades de analisis, de ocho (8 documentos (convenios, acuerdos y declaraciones que constituyeron en una muestra intencional de un total de 16 documentos seleccionados como poblacion inicial que conforman las bases de los pactos que ha suscrito la comunidad Andina de Naciones. Con referencia al analisis e interpretacion de los resultados, se constituyem en hallazgos los planteamientos ubicados en cada uno de los documentos estudiados confirmandose el papel central que juega la industria cultural para la integracion Sub-regional Andina, a pesar de no ser explico en algunos de los documentos.

  5. Implementation of a new PC based controller for a PUMA robot

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    This paper describes the replacement of a controller for a programmable universal machine for assembly (PUMA) 512 robot with a newly designed PC based (open architecture) controller employing a real-time direct control of six joints. The original structure of the PUMA robot is retained. The hardware of the new controller includes such in-house designed parts as pulse width modulation (PWM) amplifiers, digital and analog controllers, I/O cards, signal conditioner cards, and 16-bit A/D and D/A boards.An Intel Pentium Ⅳ industrial computer is used as the central controller. The control software is implemented using VC++ programming language. The trajectory tracking performance of all six joints is tested at varying velocities. Experimental results show that it is feasible to implement the suggested open architecture platform for PUMA 500 series robots through the software routines running on a PC. By assembling controller from off-the-shell hardware and software components, the benefits of reduced and improved robustness have been realized.

  6. Primeira e segunda geração de jovens imigrantes argentinos, bolivianos e peruanos em São Paulo: um estudo psicossocial da identidade e aculturação

    OpenAIRE

    Huayhua, Gladys Llajaruna

    2007-01-01

    Esta pesquisa analisou o estudo psicossocial da identidade e o processo de aculturação dos jovens imigrantes argentinos, bolivianos e peruanos de primeira e segunda geração que moram em São Paulo. Foram considerados os jovens que tinham entre 17 a 30 anos de idade, e que morem neste país há pelo menos dois anos. O estudo baseou-se na teoria de estratégias de aculturação e a psicologia intercultural de Berry, da mesma forma à teoria de grupo e o preconceito de Tajfel, reflexões de Marti...

  7. Anatomical study of the forearm and hand nerves of the domestic cat ( Felis catus), puma ( Puma concolor) and jaguar ( Panthera onca).

    Science.gov (United States)

    Sánchez, H L; Silva, L B; Rafasquino, M E; Mateo, A G; Zuccolilli, G O; Portiansky, E L; Alonso, C R

    2013-04-01

    The innervation of the forearm and hand regions of cats has not been well described despite its importance for any surgery or any neurological disorder. It is probably the main area where disorders of peripheral nerves in this species are observed. In felines, the forelimbs facilitate the jump and represent the most important way for capturing prey. The main muscles and nerves involved in this activity are located in the region of the forearm and hand. The aim of the present study was to provide a detailed description of the innervation of the forearm and hand regions of the jaguar and puma, in comparison with that of the domestic cat, contributing thus with the anatomical knowledge of the area for applying it to surgery and pathology. The forearms of three pumas and two jaguars (all of them fixed in formalin) and of six domestic cats (fresh) were dissected. The nerves path and their forearm distribution patterns of all three species were described. The analysed results indicate that the observed variations between species are minimal; thus, the anatomy described for domestic cats can be widely applied to American wild felids.

  8. Do attacks by jaguars Panthera onca and pumas Puma concolor (Carnivora: Felidae) on livestock correlate with species richness and relative abundance of wild prey?

    Science.gov (United States)

    Burgas, Albert; Amit, Ronit; Lopez, Bernat C

    2014-12-01

    Abstract: Attacks by big cats on livestock are one of the major causes of human-felid conflicts and, therefore, an important factor in the conservation of these species. It has been argued that a reduction in natural prey abundance promotes attacks on domestic species, but few studies have tested this statement, and some have delivered contradictory results. We investigated whether the occurrence of attacks to livestock by jaguar and puma relates to the abundance and richness of their natural prey. In the rainy season 2009, we tracked potential prey species counting signs of presence along linear transects in 14 non-attacked cattle farms (control) and in 14 attacked cattle farms in NW Costa Rica. There was a negative relationship between the occurrence of attacks and both species richness (p = 0.0014) and abundance (p = 0.0012) of natural prey. Our results support the establishment of actions to promote support and recovery of natural prey, in order to diminish attacks on livestock, while maintaining jaguar and puma populations.

  9. Lo que a nadie asusta pero a todos preocupa. Etnografía de los miedos cotidianos en una comunidad del altiplano sur boliviano

    Directory of Open Access Journals (Sweden)

    Gil García, Francisco M.

    2014-12-01

    Full Text Available From the idea that fear is installed in our lives, I analyze the double talk of fears denied and concerns recognized from which the inhabitants of a community in the Bolivian high-plateau explain their daily lives, paying particular attention to the reasons given by adults in the education of children when being in certain environments or forbid them to perform certain actions. So, I will present a spatial distinction among urban settings: the own spaces, secure and recognized —but potentially threatened—, and the territory of the others, which the concurrency of past and present terrible otherness transforms in a frightening geography where things happen, fear scenarios that need to be conspirators and reordered.Partiendo de la idea de que el miedo existe instalado en nuestras vidas, se analiza el doble discurso de miedos negados y preocupaciones reconocidas desde el que los habitantes de una comunidad del altiplano boliviano explican su vida cotidiana, prestando especial atención a las razones esgrimidas por los adultos en la educación de los niños a la hora de prohibirles frecuentar determinados ambientes o realizar en ellos ciertas acciones. Para ello partiré de una distinción espacial dentro del entramado urbano, diferenciando así entre espacios propios, seguros y reconocidos —pero potencialmente amenazados— que, debido a la concurrencia de determinadas alteridades terribles presentes y pretéritas, son sentidos como un territorio de los otros, una geografía atemorizadora donde suceden cosas, escenarios del miedo que necesitan ser conjurados y reordenados.

  10. Expression of PUMA in Follicular Granulosa Cells Regulated by FoxO1 Activation During Oxidative Stress.

    Science.gov (United States)

    Liu, Ze-Qun; Shen, Ming; Wu, Wang-Jun; Li, Bo-Jiang; Weng, Qian-Nan; Li, Mei; Liu, Hong-Lin

    2015-06-01

    Many studies have demonstrated that oxidative stress-induced apoptosis is a main cause of follicular atresia. Reactive oxygen species (ROS)-induced granulosa cell (GC) apoptosis is regulated by a variety of signaling pathways involving numerous genes and transcription factors. In this study, we found expression of the p53-upregulated modulator of apoptosis (PUMA), a BH3-only Bcl-2 subfamily protein, in ovarian GCs during oxidative stress. By overexpression and knockdown of Forkhead box O1 (FoxO1), we found that FoxO1 regulates PUMA at the protein level. Moreover, as c-Jun N-terminal kinase (JNK) has been shown to activate FoxO1 by promoting its nuclear import, we used a JNK inhibitor to reduce FoxO1 activation and detected decreased PUMA messenger RNA expression and protein levels during oxidative stress. In addition, in vivo oxidative stress-induced upregulation of PUMA was found following injection of 3 nitropropionic acid in mice. In conclusion, oxidative stress increases PUMA expression regulated by FoxO1 in follicular GCs.

  11. El Contrato de Agencia Comercial y los Cambios Introducidos en la Negociación del TLC Entre EE.UU. y los Países Andinos

    Directory of Open Access Journals (Sweden)

    Adriana López

    2011-07-01

    Full Text Available Tomando en consideración que dentro del tratado de libre comercio entre los Países Andinos y los Estados Unidos, se negociaron algunos cambios importantes en la regulación sobre el contrato de agencia comercial, ésta investigación tiene como objetivo principal, mostrar cuales fueron los puntos negociados por los Estados Unidos y por Colombia para este contrato, y exponer cuáles serán los cambios que el Congreso colombiano tendrá que introducir al Código de Comercio, en materia de agencia comercial.

  12. El Contrato de Agencia Comercial y los Cambios Introducidos en la Negociación del TLC Entre EE.UU. y los Países Andinos

    Directory of Open Access Journals (Sweden)

    Adriana López

    2011-06-01

    Full Text Available Tomando en consideración que dentro del tratado de libre comercio entre los Países Andinos y los Estados Unidos, se negociaron algunos cambios importantes en la regulación sobre el contrato de agencia comercial, ésta investigación tiene como objetivo principal, mostrar cuales fueron los puntos negociados por los Estados Unidos y por Colombia para este contrato, y exponer cuáles serán los cambios que el Congreso colombiano tendrá que introducir al Código de Comercio, en materia de agencia comercial.

  13. Las Orogénesis del Paleozoico Inferior en el margen proto-andino de América del Sur, Sierras Pampeanas, Argentina

    OpenAIRE

    Rapela, Carlos W.; Casquet, César; Baldo, Edgardo G.; Juan A Dahlquist; Pankhurst, R. J.; Galindo Francisco, Mª del Carmen; Saavedra, Julio

    2001-01-01

    El margen proto-andino de Gondwana ha sido el escenario de al menos dos orogénesis desde el desmembramiento del supercontinente Rodinia al final del Neoprotrozoico, hasta el reagrupamiento de las masas continentales en Pangea al final del Carbonifero. Ambas orogénesis van precedidas de un periodo de apertura oceánica y sedimentación en márgenes pasivos y culminan en subducción oceánica con desarrollo de arcos-magmáticos de tipo cordillerano y colision de tipo continente-cont...

  14. Oxygen isotope composition of North American bobcat (Lynx rufus) and puma (Puma concolor) bone phosphate: implications for provenance and climate reconstruction.

    Science.gov (United States)

    Pietsch, Stephanie J; Tütken, Thomas

    2016-01-01

    Feline carnivores are threatened by illegal wildlife trade. Tracing the provenance of unknown felid tissues via stable isotope analysis could provide important information in wildlife crime investigations. The oxygen isotope composition of mammalian skeletal phosphate (δ(18)Op) is widely applied to trace the origin of animal remains and to reconstruct migratory patterns in palaeontological, archaeological, ecological and wildlife forensic applications. Teeth and bones of terrestrial mammals form at constant body temperature in isotope equilibrium with body water, which is predominantly controlled by ingested meteoric water (δ(18)Ow) that varies systematically with latitude, altitude and climate. Here we analysed δ(18)Op of 106 North American puma and bobcat bones of known geographic origin to establish the first δ(18)Op-δ(18)Ow regression for feline carnivores: δ(18)Op = 0.40(±0.04) * δ(18)Ow + 20.10(±0.40) (R(2) = 0.46, n = 106). This was compared with those from their respective prey species (deer and rabbit), a canid carnivore (fox) and other placental mammals. Effects of species, sex and relative humidity on the feline δ(18)Op-δ(18)Ow correlation were analysed and additional intra-individual tissue comparisons (hair δ(18)Oh vs. bone δ(18)Op) were performed for some bobcat individuals. Bobcats and pumas exhibited only a moderate δ(18)Op-δ(18)Ow correlation, which differed from canid carnivores and other placental mammals. However, feline δ(18)Op values revealed a moderate relation with δ(18)Ow, which lacks for the δ(18)Oh of hair from the same bobcat individuals. This indicates a difference in oxygen isotope routing from body water to bioapatite and hair. Most herbivores and omnivores track δ(18)Ow in their bioapatite δ(18)Op values much better, whereas δ(18)Op and especially δ(18)Oh values of feline carnivores are less precise proxies for meteoric water δ(18)Ow values and thus for provenance determination in wildlife

  15. Impact of Burnout and Psychosocial Work Characteristics on Future Long-Term Sickness Absence. Prospective Results of the Danish PUMA Study Among Human Service Workers

    NARCIS (Netherlands)

    Borritz, Marianne; Christensen, Karl Bang; Bultmann, Ute; Rugulies, Reiner; Lund, Thomas; Andersen, Ingelise; Villadsen, Ebbe; Diderichsen, Finn; Kristensen, Tage S.

    2010-01-01

    Objectives: The objective of this study was to examine if burnout and psychosocial factors predicted long-term sickness absence (>2 weeks) at work unit level. Methods: Data were collected prospectively at 82-work units in human services (PUMA cohort, PUMA: Danish acronym for Burnout, Motivation and

  16. The proapoptotic BH3-only proteins Bim and Puma are downstream of endoplasmic reticulum and mitochondrial oxidative stress in pancreatic islets in response to glucotoxicity.

    Science.gov (United States)

    Wali, J A; Rondas, D; McKenzie, M D; Zhao, Y; Elkerbout, L; Fynch, S; Gurzov, E N; Akira, S; Mathieu, C; Kay, T W H; Overbergh, L; Strasser, A; Thomas, H E

    2014-03-13

    Apoptosis of pancreatic beta cells is a feature of type 2 diabetes and its prevention may have therapeutic benefit. High glucose concentrations induce apoptosis of islet cells, and this requires the proapoptotic Bcl-2 homology domain 3 (BH3)-only proteins Bim and Puma. We studied the stress pathways induced by glucotoxicity in beta cells that result in apoptosis. High concentrations of glucose or ribose increased expression of the transcription factor CHOP (C/EBP homologous protein) but not endoplasmic reticulum (ER) chaperones, indicating activation of proapoptotic ER stress signaling. Inhibition of ER stress prevented ribose-induced upregulation of Chop and Puma mRNA, and partially protected islets from glucotoxicity. Loss of Bim or Puma partially protected islets from the canonical ER stressor thapsigargin. The antioxidant N-acetyl-cysteine also partially protected islets from glucotoxicity. Islets deficient in both Bim and Puma, but not Bim or Puma alone, were significantly protected from killing induced by the mitochondrial reactive oxygen species donor rotenone. Our data demonstrate that high concentrations of glucose induce ER and oxidative stress, which causes cell death mediated by Bim and Puma. We observed significantly higher Bim and Puma mRNA in islets of human donors with type 2 diabetes. This indicates that inhibition of Bim and Puma, or their inducers, may prevent beta-cell destruction in type 2 diabetes.

  17. El páramo andino: características territoriales y estado ambiental. Aportes interdisciplinarios para su conocimiento

    Directory of Open Access Journals (Sweden)

    Serrano Giné, David

    2015-06-01

    Full Text Available The most important characteristics of the Andean páramo are presented, an ecosystem of social and territorial interest, little known among Spaniard geographers. The synthesis developed constitutes a state of the art, updated and contrasted of the main appearances that define and characterise this space. The adopted approach has a systemic character and focuses in environmental questions, for being those ones who better embraces the páramo’s current complexity. After summarizing the geographical setting of the Andean páramo, we review the subjects that better describe its social profit, which includes hydrological uses, land cover and land use activities carried by locals. The main purpose of this article is to let know to the Iberian geographers a subject of great actuality among Latin American geographers.Se presentan las características generales del páramo andino, un ecosistema de gran interés social y territorial poco conocido entre los geógrafos españoles. La síntesis desarrollada constituye un estado de la cuestión actualizado y contrastado sobre los principales aspectos que definen y caracterizan este espacio. La perspectiva adoptada tiene un marcado carácter sistémico y focaliza en cuestiones de orden ambiental, por ser este aspecto el que mejor engloba la complejidad actual del páramo. Así, después de glosar el encuadre geográfico del páramo andino, se repasa de forma sumaria los aspectos que mejor describen el beneficio social de este ámbito, a saber los aprovechamientos hídricos, la ocupación del suelo y el uso que realizan las sociedades implicadas. El propósito principal de este artículo es dar a conocer a los geógrafos ibéricos un ámbito de trabajo de gran actualidad entre los geógrafos latinoamericanos. [fr] Cet article expose les caractéristiques générales du páramo andin : un écosystème de grand intérêt social et territorial peu connu des géographes espagnols. Cet article constitue un

  18. Características de la avifauna en un gradiente altitudinal de un bosque nublado andino en La Paz, Bolivia

    Directory of Open Access Journals (Sweden)

    Omar Martínez

    2013-04-01

    Full Text Available El presente trabajo analiza la diversidad y composición de la avifauna en tres localidades ubicadas dentro del Parque Nacional y Área Natural de Manejo Integrado de Cotapata, La Paz, Bolivia. Las tres localidades se distribuyeron en gradiente altitudinal y tres tipos de bosques húmedos andinos (ceja de monte a 3170 m, bosque nublado a 2620 m y bosque nublado secundario a 1850 m de la zona Yungas. Se realizó un esfuerzo total de 3286 horas-red niebla, 324 horas de caminatas y 216 horas de conteos por puntos en los tres lugares de estudio. Un total de 220 especies fueron registradas, la mayoría capturadas en redes (100 spp., 45%. Veinticinco familias fueron representadas en las capturas por redes y 42 mediante los censos. Tyrannidae y Thraupidae fueron las más representativas mediante ambos métodos. Las curvas acumulativas de especies fueron similares en todos los sitios. La riqueza de especies para la ceja de monte, bosque nublado y bosque secundario a partir de las capturas y censos fueron de 44, 40, 44 especies y 69, 57, 86 especies, respectivamente. Un total de 16 especies de rango restringido fueron registradas (p.e. Odontophorus balliviani, Andigena cucullata. La diversidad para la ceja de monte fue H´= 1,41; para el bosque nublado (H´= 0,98 y el bosque nublado secundario (H´= 0,96. La diversidad beta fue 0,74 basada en datos de capturas con redes y 0,79 basada en datos de censos en las tres alturas.

  19. GRUPOS FUNCIONALES DE PLANTAS EN BORDES DE AVANCE CON POTENCIAL PARA LA RESTAURACIÓN DE UN BOSQUE ALTO ANDINO

    Directory of Open Access Journals (Sweden)

    Bonilla Maria Argenis

    2011-04-01

    Full Text Available El estudio de grupos funcionales de plantas es una herramienta útil en la identificación de características ecológicas de importancia en la regeneración de una comunidad. El objetivo de este estudio fue la identificación de grupos funcionales de plantas en bordes de avance de un bosque alto andino y la evaluación de su importancia en el curso de la sucesión secundaria en pastizales abandonados. Con base en levantamientos de vegetación de 10 x 10m y en la revisión de atributos vitales de las especies registradas, se realizó una clasificación multivariante de las especies en grupos emergentes de plantas. Los atributos más importantes para la clasificación de los grupos emergentes fueron el método de dispersión, la formación de un banco de semillas y la presencia de propagación vegetativa y asociada a esta la ramificación basal de tallo. Se definieron tres grupos para las especies de estrato herbáceo y tres para las del estrato arbustivo-arbóreo, los cuales reúnen especies con estrategias morfo-fisiológicas similares. Dentro de los grupos definidos, las especies herbáceas dispersadas por diversos medios abióticos y las especies arbustivas con ramificación basal dispersadas por viento y por aves parecen ser claves en la colonización del terreno dominado por H. lanatus y en la facilitación del establecimiento de especies de bosque secundario.

  20. Validación y aplicación de la prueba ELISA para medir cortisol fecal en jaguar (Panthera onca) y puma (Puma concolor) durante un programa de enriquecimiento ambiental en el Zoológico Jaime Duque

    OpenAIRE

    Catalina Rodríguez Álvarez; Leonardo Arias Bernal

    2005-01-01

    El enriquecimiento ambiental busca aumentar el bienestar de los animales cautivos mediante la provisión de estímulos que motiven la realización de comportamientos típicos de la especie. A las poblaciones de jaguares (Panthera onca) y pumas (Puma concolor) presentes en el Zoológico Jaime Duque se les aplicó un programa de enriquecimiento ambiental y con el fin de probar si su bienestar aumentaba, se realizaron mediciones de cortisol en materia fecal mediante la prueba de ELISA, para lo cual hu...

  1. Targeting miR-155 suppresses proliferation and induces apoptosis of HL-60 cells by targeting Slug/PUMA signal.

    Science.gov (United States)

    Liang, Hui; Dong, Ziyan; Liu, Jiang-Feng; Chuang, Wei; Gao, Li-Zhen; Ren, Yu-Guo

    2016-10-27

    Recent studies have shown that high miR-155 expression was associated with poor prognosis in patients with acute myelogeneous leukemia (AML). Furthermore, targeting miR-155 results in monocytic differentiation and apoptosis. However, the exact role and mechanisms of miR-155 in human AML remains speculative. HL-60 cells were treated with anti-miR-155 for 72 h. Cell growth and apoptosis in vitro were detected by MTT, BrdU proliferation, colony formation and flow cytometry assay. The effect of anti-miR-155 on growth of HL-60 cells was also evaluated in a leukemia mouse model. Slug cDNA and PUMA siRNA trannsfection was used to assess the signal pathway. Different protein expression was detected by western blot assay and quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) assay. The results shown that targeting miR-155 resulted in a 24-fold decrease of miR-155 expression compared to negative control in the HL-60 cells. Targeting miR-155 significantly downregulated Slug and upregulated PUMA expression, and decreased HL-60 cell growth by 70% , impaired colony formation by approximately 60%, and increased HL-60 cell apoptosis by 45%. Targeting PUMA reversed miR-155 sliencing-induced proliferation and apoptosis of HL-60 cells. Restoration of Slug decreased PUMA expression. In murine engraftment models of HL-60 cells, we showed that targeting miR-155 was able to reduce tumor growth. This was accompanied with decreased Slug expression and increased PUMA expression in these tumors. Collectively, our findings strongly suggest targeting miR-155 exhibited in vivo and in vitro antileukemic activities in AML through a novel mechanism resulting in inhibition of Slug expression and increase of PUMA expression.

  2. PUMA and NF-kB Are Cell Signaling Predictors of Reovirus Oncolysis of Breast Cancer

    Science.gov (United States)

    Shi, Zhong-Qiao; Thirukkumaran, Ponnampalam; Luider, Joanne; Kopciuk, Karen; Spurrell, Jason; Elzinga, Kate; Morris, Don

    2017-01-01

    Background and purpose Reovirus is a ubiquitous RNA virus that exploits aberrant signaling pathways for its replication. The oncolytic potential of reovirus against numerous cancers under pre-clinical/clinical conditions has been documented by us and others. Despite its proven clinical activity, the underlying mechanisms of reovirus oncolysis is still not well elucidated. If reovirus therapy is to be optimized for cancer, including breast cancer patients, it is imperative to understand the mechanisms of reovirus oncolysis, especially in treatment of resistant tumour. Experimental approach and results In the present study global gene expression profiling was utilized as a preliminary roadmap to tease-out pivotal molecules involved in reovirus induced apoptosis in breast cancer. Reovirus treated HTB133 and MCF7 breast cancer cells revealed transcriptional alteration of a defined subset of apoptotic genes and members of the nuclear factor-kappa B (NF-kB) family and p53 upregulated modulator of apoptosis (PUMA) were prominent. Since NF-kB can paradoxically suppress or promote apoptosis in cancer, the significance of NF-kB in reovirus oncolysis of breast cancer was investigated. Real time PCR analysis indicated a 2.9–4.3 fold increase in NF-kB p65 message levels following reovirus infection of MCF7 and HTB133, respectively. Nuclear translocation of NF-kB p65 protein was also dramatically augmented post reovirus treatment and correlated with enhanced DNA binding. Pharmacologic inhibition of NF-kB lead to oncolytic protection and significant down regulation of PUMA message levels. PUMA down regulation using siRNA suppressed reovirus oncolysis via significantly repressed apoptosis in p53 mutant HTB133 cells. Conclusions This study demonstrates for the first time that a prominent pathway of reovirus oncolysis of breast cancer is mediated through NF-kB and that PUMA upregulation is dependent on NF-kB activation. These findings represent potential therapeutic indicators of

  3. 同域栖息的美洲虎和美洲狮存在竞争吗?%Is there competition between sympatric jaguar Panthera onca and puma Puma concolor ?

    Institute of Scientific and Technical Information of China (English)

    Aaron M. HAINES

    2006-01-01

    美洲虎(Panthera onca)和美洲狮(Puma concolor)是中美洲和南美洲大部区域共存的大型猫科动物,其共存理论得到研究验证.本文作者回顾了有关文献,认为同域分布的美洲虎和美洲狮存在生境和猎物分化,提出热带美洲狮具有较小体型以利用较小的猎物并减少与美洲虎竞争的假设.研究支持美洲虎和美洲狮的空间相互回避概念,但两者猎物之间分化的研究结果不相吻合.尽管如此,文献认为新热带界美洲狮体型较小只是反映了在进化过程中偏于利用较小猎物的事实,而与美洲虎的竞争无关.人类的影响会引起美洲虎和美洲狮的共存.在新热带界环境中,美洲虎由于依赖特定猎物而较大程度地受到人类引起的猎物种群下降之影响,但美洲狮由于可以偏向利用小型猎物或猎物多样化而得到补偿,从而能更好地适应人类的影响[动物学报52(6):1142-1147,2006].%The jaguar Panthera onca and puma Puma concolor are large felids that coexist throughout Central and most of South America. Studies have tested theories of coexistence of sympatric jaguar and puma. By reviewing the scientific literature, I assessed whether habitat or prey are partitioned between sympatric jaguar and puma, and hypothesized that puma in the tropics have a smaller body size to select smaller prey items to minimize competition with jaguar. Studies support the concept of mutual spatial avoidance between jaguar and puma, whereas results of prey partitioning between jaguar and puma are conflicting. Despite these relationships, the scientific literature suggests that smaller puma in the Neotropics may simply reflect the fact that they have evolved to concentrate on smaller prey species regardless of competition with jaguar.However, anthropogenic impacts can cause changes between jaguar and puma coexistence. The jaguar may suffer from declining prey populations caused by humans because of predator

  4. Nodulação em cultivares de feijão dos conjuntos gênicos andino e meso-americano

    Directory of Open Access Journals (Sweden)

    Franco Marília Caixeta

    2002-01-01

    Full Text Available O objetivo deste trabalho foi determinar a capacidade de nodulação e a especificidade de feijão (Phaseolus vulgaris L. dos conjuntos gênicos andino e meso-americano submetidos a inóculo de Rhizobium. O experimento foi estabelecido em parcelas subdivididas em blocos ao acaso, com quatorze cultivares de feijão e três estirpes de Rhizobium (R. etli KIM 5, R. etli CIAT 632 e R. tropici CIAT 899. Somente as cultivares andinas WAF 15, WAF 7, Mineiro Precoce, WAF 6 e Antioquia 8 apresentaram especificidade na nodulação. Em relação à massa seca dos nódulos, houve diferenças significativas dos tratamentos de inoculação nas cultivares andinas WAF 15, WAF 7, WAF 6 e Diacol Andino, e na cultivar meso-americana Ouro Negro. Nenhuma das cultivares restringiu a nodulação, embora tenham sido verificadas diferenças de até 53 e 103 vezes no número e massa de nódulos por planta, respectivamente. Considerando todas as cultivares e estirpes de rizóbio, WAF 15 foi a cultivar com melhor desempenho em número e massa nodular. WAF 6 foi a cultivar de pior desempenho, chegando quase ao nível de restrição da nodulação com a estirpe R. etli CIAT 632.

  5. El campesino andino y la globalización a fines de siglo (una mirada sobre el caso ecuatoriano

    Directory of Open Access Journals (Sweden)

    Luciano Martínez

    2004-10-01

    Full Text Available The Andean peasant and globalization at the end of the century  (a look at the Ecuadorian caseThis article explores the current situation of the  small landed peasantry in the Ecuadorian Andes,  confronted by important changes in the agrarian  system, in social relations and in links with the  global market. The effects of this situation are: an  increase in small farms (which is a progressive  loss of land resources, a crisis within the supportive relationships in the community, and a rise in  international migration, which is a new process of  integration as cheap labour in the global market.  The politics of adjustment and the dolarization of the economy have made peasant production less  competitive, thus tending to cause proletarianization of labour in the national as well as the international market. From this context, the effects of  international migration trends on the social fabric  of a peasant organization from the southern Ecuadorian Andes are analyzed.Resumen:Este artículo aborda la situación actual del campesinado minifundista andino del Ecuador, quien  enfrenta importantes cambios en la estructura  agraria, en las relaciones sociales y en la vinculación con el mercado global. Esta situación se  manifiesta a través de: el incremento del minifundio (que implica la pérdida progresiva del recurso  de la tierra, la crisis de las relaciones solidarias  en las comunidades, y un aumento de la migración internacional – un nuevo proceso de integración como mano de obra barata en el mercado mundial. Con las políticas de ajuste y la dolarización de la economía, la producción campesina se  ha convertido poco competitiva, lo que a su vez  tiende a proletarizar la mano de obra tanto en el  mercado nacional como en el internacional. Desde  este contexto, se analizan los efectos de la migración internacional en el tejido social de una organización campesina en el sur de la sierra ecuatoriana.

  6. Ectoparasites of free-ranging pumas and jaguars in the Paraguayan Chaco.

    Science.gov (United States)

    Durden, Lance A; Cunningham, Mark W; McBride, Rocky; Ferree, Bambi

    2006-04-15

    Ectoparasites were collected from seven puma (Puma concolor) and seven jaguar (Panthera onca) live-captures (each representing six different animals) in the Paraguayan Chaco from 2002 to 2004. The same five species of ectoparasites were recovered from both host species: the flea, Pulex simulans (total on both hosts combined=30 male, 49 female), and the ticks, Amblyomma cajennense (1 male, 4 female, 46 nymphs, 241 larvae), Amblyomma parvum (42 male, 25 female), Amblyomma tigrinum (1 male, 7 female, 34 larvae) and Amblyomma triste (4 male). There were no statistical differences between the prevalences (percent of hosts infested) for any of these ectoparasite species between the two host species, and only P. simulans showed a significantly higher mean intensity (mean no. of each ectoparasite species per infested host) on one of the host species (jaguar). Clearly, these two large carnivores share similar ectoparasite faunas in the Paraguayan Chaco. However, both carnivores occupy large geographical distributions in the New World and this study, combined with the few previous studies, suggests that their ectoparasite faunas differ slightly in different parts of their respective ranges.

  7. Biosynthesized Silver Nanoparticles Used in Preservative Solutions for Chrysanthemum cv. Puma

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    Luis M. Carrillo-López

    2016-01-01

    Full Text Available The use of pulse solutions containing antimicrobials has been reported, but more research is necessary. To increase vase life and to study their effect on opening inflorescences, silver nanoparticles were used in vase solutions for cv. Puma Chrysanthemum stems. The nanoparticles were synthesized biologically using Chenopodium ambrosioides L. applied at concentrations of 0.01, 0.05, 0.1, 0.5, 1, and 5 mM and compared with a control. Treatments were replicated five times. The stems were cut to 50 cm and observed until the end of their vase life. Low concentrations of silver nanoparticles promoted inflorescence opening and leaf yellowing, while the control leaves remained green, but there was a lower degree of inflorescence opening. High concentrations of silver nanoparticles (0.5, 1, and 5 mM caused senescence due to low water uptake through the stems. Statistical differences in inflorescence opening and diameter, bacterial growth (CFU mL−1 in vase solutions, fresh weight, water uptake, and vase life were found among treatments. Longer vase life and less weight loss were observed in the stems exposed to low concentrations of silver nanoparticles. Low concentrations of silver nanoparticles promoted inflorescence opening and increased vase life of Chrysanthemum cv. Puma.

  8. A new atypical genotype mouse virulent strain of Toxoplasma gondii isolated from the heart of a wild caught puma (Felis concolor) from Durango, Mexico.

    Science.gov (United States)

    Dubey, J P; Alvarado-Esquivel, C; Herrera-Valenzuela, V H; Ortiz-Diaz, J J; Oliveira, S; Verma, S K; Choudhary, S; Kwok, O C H; Su, C

    2013-11-08

    Nothing is known of the genetic diversity of Toxoplasma gondii circulating in wildlife in Mexico. In the present study, a mouse virulent T. gondii strain was isolated from the heart of a wild puma (Felis concolor). The puma was found roaming in outskirt of Durango City, Mexico and tranquilized for moving to a zoo. The puma died during translocation and a necropsy examination was performed. The puma had an antibody titer for T. gondii of 200 by the modified agglutination test. Its heart and brain tissue were bioassayed into 2 outbred Swiss Webster (SW) and 1 gamma interferon gene knockout (KO) mouse. The KO mouse and the 2 SW mice that became infected after inoculation with homogenate of puma heart died of acute toxoplasmosis 12, 19 and 20 days p.i. respectively and tachyzoites were found in lungs of all 3 mice. None of the 4 SW and 1 KO mouse inoculated with digest of the puma brain became infected with T. gondii. Tachyzoites from the lungs of mice were propagated in cell cultures. Tachyzoites from cell culture were inoculated into 5 SW; the mice died or had to be killed 14 days p.i. and a cat fed tissues of these mice shed T. gondii oocysts. Results of mortality and infectivity of tachyzoites and oocysts in SW mice indicated that the puma T. gondii strain (designated TgPumaMe1) was virulent for outbred mice. DNA isolated from culture-derived tachyzoites was characterized using 11 PCR-RFLP markers (SAG1, 5'- and 3'-SAG2, alt.SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1 and Apico) revealed a new genotype (ToxoDB PCR-RFLP #222). Isolation of atypical genotype T. gondii from wild puma indicates that mouse virulent strains are circulating in wildlife in Mexico.

  9. 促凋亡基因puma的研究进展%Research Progress of Proapoptic Gene puma

    Institute of Scientific and Technical Information of China (English)

    宋宇

    2011-01-01

    p53 up-regulated modulator of apoptosis( puma )acts as an essential mediator of apoptosis via p53 -dependent and p53 -independent mechanisms, which may play a prominent role in the formation , development,treatment of tumors and autoimmune diseases. It can be potentially used as a new target for anticancer and apoptosis-associated diseases therapy. Here is to review its structural characteristics, function, pro-apoptotic role, chemical drugs influence on the expression of puma in several cell lines, other transcription pathways regulation and post-transcription regulation of puma.%p53上调凋亡调控因子(puma)可通过p53依赖途径和p53非依赖途径诱导多种肿瘤细胞的凋亡,可能在肿瘤及自身免疫性疾病的发生、发展和治疗中发挥重要的作用,并有望成为人类肿瘤和凋亡相关疾病治疗的新靶点.现就其结构和功能、促凋亡作用、化学药物对多种细胞株中puma表达水平的影响、其他转录途径调节以及转录后调节puma予以综述.

  10. Over-expression of PUMA correlates with the apoptosis of spinal cord cells in rat neuropathic intermittent claudication model.

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    Bin Ma

    Full Text Available BACKGROUND: Neuropathic intermittent claudication (NIC is a typical clinical symptom of lumbar spinal stenosis and the apoptosis of neurons caused by cauda equina compression (CEC has been proposed as an important reason. Whereas, the factors and the mechanism involved in the process of apoptosis induced by CEC remain unclear. METHODOLOGY AND RESULTS: In our modified rat model of NIC, a trapezoid-shaped silicon rubber was inserted into the epidural space under the L5 and L6 vertebral plate. Obvious apoptosis was observed in spinal cord cells after compression by TUNEL assay. Simultaneously, qRT-PCR and immunohistochemistry showed that the expression levels of PUMA (p53 up-regulated modulator of apoptosis and p53 were upregulated significantly in spinal cord under compression, while the expression of p53 inhibitor MDM2 and SirT2 decreased in the same region. Furthermore, CEC also resulted in the upregulation of Bcl-2 pro-apoptotic genes expression and caspase-3 activation. With the protection of Methylprednisolone, the upregulation of PUMA and p53 expression as well as the decrease of MDM2 and SirT2 in spinal cord were partially rescued in western bolt analysis. CONCLUSIONS: These results suggest that over-expression of PUMA correlates with CEC caused apoptosis of spinal cord cells, which is characterized by the increase of p53, Bax and Bad expression. PUMA upregulation might be crucial to induce apoptosis of spinal cord cells through p53-dependent pathway in CEC.

  11. Anthropometry of height, weight, arm, wrist, abdominal circumference and body mass index, for Bolivian adolescents 12 to 18 years: Bolivian adolescent percentile values from the MESA study Referencias antropométricas de los adolescentes bolivianos de 12 a 18 años: estatura, peso, circunferencia del brazo, muñeca y abdominal, índice de masa corporal: Percentiles de adolescentes bolivianos (PAB del estudio MESA

    Directory of Open Access Journals (Sweden)

    A. Baya Botti

    2009-06-01

    Full Text Available Anthropometry is important as clinical tool for individual follow-up as well as for planning and health policymaking at population level. Recent references of Bolivian Adolescents are not available. The aim of this cross sectional study was to provide age and sex specific centile values and charts of Body Mass Index, height, weight, arm, wrist and abdominal circumference from Bolivian Adolescents. Data from the MEtabolic Syndrome in Adolescents (MESA study was used. Thirty-two Bolivian clusters from urban and rural areas were selected randomly considering population proportions, 3445 school going adolescents, 12 to 18 y, 45% males; 55% females underwent anthropometric evaluation by trained personnel using standardized protocols for all interviews and examinations. Weight, height, wrist, arm and abdominal circumference data were collected. Body Mass Index was calculated. Smoothed age- and gender specific 3rd, 5th, 10th, 25th, 50th, 75th, 85th, 90th, 95th and 97th Bolivian adolescent percentiles(BAP and Charts(BAC where derived using LMS regression. Percentile-based reference data for the antropometrics of for Bolivian Adolescents are presented for the first time.La antropometría es una herramienta clínica importante para el seguimiento individual de los pacientes así como para la planificación de políticas públicas. En Bolivia no existen referencias antropométricas nacionales para adolescentes. El objetivo de este estudio transversal fue de desarrollar percentiles y diagramas de crecimiento para peso, talla, índice de masa corporal, presión arterial sistólica y diastólica, circunferencia de muñeca, brazo y abdominal de adolescentes bolivianos. Los datos antropométricos en el estudio MESA (Síndrome metabólico en adolescentes bolivianos fueron obtenidos a partir de 32 unidades muestrales, considerando proporcionalidad muestral con reposición. Fueron evaluados 3445 adolescentes de 12 a 18, 45% hombres; 55% mujeres, de colegios de

  12. Estrogen receptor β upregulates FOXO3a and causes induction of apoptosis through PUMA in prostate cancer.

    Science.gov (United States)

    Dey, P; Ström, A; Gustafsson, J-Å

    2014-08-14

    Estrogen receptor β (ERβ) is emerging as a critical factor in understanding prostate cancer biology. Although reduced in prostate cancer above Gleason grade 3, ERβ is a potential drug target at the initial stage of the disease. In human prostate cancer cells, we found that ERβ causes apoptosis by increasing the expression of pro-apoptotic factor p53-upregulated modulator of apoptosis (PUMA), independent of p53, but dependent on the forkhead transcription factor class-O family member, FOXO3a. FOXO3a has previously been shown to induce PUMA after growth factor withdrawal and inhibition of the Akt pathway. Surprisingly, the phosphorylation of FOXO3a remained unchanged, while the mRNA and total protein levels of FOXO3a were increased in response to ERβ expression or treatment of PC3, 22Rv1 and LNCaP cells with the ERβ-specific ligands 3β-Adiol (5α-androstane-3β,17β-diol), DPN (diarylpropionitrile) or 8β-VE2 (8-vinylestra-1,3,5 (10)-triene-3,17β-diol). Knockdown of FOXO3a or ERβ expression abolished the increase of PUMA in response to 3β-Adiol in LNCaP and PC3 cells, suggesting that FOXO3a mediates the apoptotic effect of 3β-Adiol-activated ERβ. Moreover, the ventral prostate of ERβ-/- mice had decreased expression of FOXO3a and PUMA compared with the ERβ+/+ mice, indicating a relationship between ERβ and FOXO3a expression. The regulation of FOXO3a by ERβ in normal basal epithelial cells indicates a function of ERβ in cell differentiation and maintenance of cells in a quiescent state. In addition, the expression of ERβ, FOXO3a and PUMA is comparable and higher in benign prostatic hyperplasia than in prostate cancer Gleason grade 4 or higher, where there is substantial loss of ERβ, FOXO3a and PUMA. We conclude that ERβ induces apoptosis of prostate cancer cells by increasing transcription of FOXO3a, leading to an increase of PUMA and subsequent triggering of apoptosis via the intrinsic pathway involving caspase-9. Furthermore, we conclude that

  13. Nacionalización de los hidrocarburos bolivianos: reflexiones sobre su impacto en lo doméstico y en los vínculos con Argentina y Brasil

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    Natalia Ceppi

    2014-01-01

    Full Text Available En mayo de 2006, Evo Morales anunció una medida de trascendencia histórica: la tercera nacionalización de los hidrocarburos bolivianos. Se elabora una serie de reflexiones sobre las principales connotaciones del Decreto Supremo 28 701 “Héroes del Chaco” para la sociedad boliviana, del cual se consideran sus aspectos más relevantes en materia económica y social. Asimismo, se analizan los cambios en los contratos de exportación de gas natural que alimentan las necesidades de energía de Argen - tina y Brasil en este siglo. Se revaloriza en el debate académico el lugar de la energía como un asunto prioritario en las agendas estatales y en el escenario internacional.

  14. LA TRANSFORMACIÓN DEL ESTADO EN AMÉRICA LATINA: UNA PERSPECTIVA ECONÓMICA DESDE LOS PAÍSES ANDINOS

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    Edgard Moncayo Jiménez

    2006-01-01

    Full Text Available Desde varias perspectivas analíticas, la soberanía del Estado-nación está siendo desbordada en tres direcciones: hacia arriba, por la globalización y la supranacionalización; horizontalmente, por el desmonte del estado de bienestar, la privatización y la regulación; y hacia abajo, por la descentralización y la devolución de autonomías a las polis regionales y locales. En este trabajo se afirma que, antes que un simple vaciamiento (hollowing out del Estado-nación, esta entidad está experimentando una profunda transformación y reescalamiento en los tres planos anotados. Para el efecto, se analiza el caso específico de los cinco países andinos, y se pone énfasis en los aspectos económicos.

  15. Densidad y preferencia de hábitat del perezoso de dos dedos Choloepus hoffmanni en un bosque andino (Bojacá, Cundinamarca, Colombia)

    OpenAIRE

    Polanco Rocío; Cadena Alberto; Álvarez Silvia Juliana

    2004-01-01

    Se estimó la densidad de perezosos de dos dedos Choloepus hoffmanni en un bosque andino fragmentado, a partir del conteo de restos fecales en parcelas fijas. Se caracterizó la vegetación del área y los árboles usados por los perezosos, con el fin de evaluar la preferencia de hábitat a dos escalas diferentes, así como la relación de las características del hábitat sobre la densidad de perezosos y la frecuencia de uso de los árboles. La densidad de perezosos en el área de estudio fue de 1,5 ind...

  16. Anfibios andinos del Perú fuera de Áreas Naturales Protegidas: amenazas y estado de conservación

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    César Aguilar

    2011-05-01

    Full Text Available Presentamos una lista actualizada de los anfibios presentes en Perú y para las especies andinas (con distribución altitudinal sobre los 1000 m de altitud se informa sobre su categoría según la UICN, los endémicos de Perú, sus amenazas y su presencia dentro del SINANPE. Hasta el año 2010 se conocen 538 especies de anfibios; 110 especies fueron registradas entre el año 2003 y el 2010; aproximadamente una especie descrita por mes. De las 110 especies descritas desde el 2003, 77 fueron andinas y 64% de éstas se encuentran fuera del SINANPE. En total son reconocidas 235 especies andinas, el 80% son endémicas de Perú y 58% de éstas se encuentran fuera del SINANPE. Noventaiun especies andinas están en categorías de amenaza y en la categoría de Casi Amenazado, y 53% de éstas se encuentran fuera del SINANPE. Los géneros Telmatobius y Atelopus, así como las familias Centrolenidae y Strabomantidae, presentan los porcentajes más altos de especies andinas con categorías de amenaza y Casi Amenazado. Para 83 (36% especies de anfibios andinos presentes en Perú, la principal amenaza es la pérdida del hábitat. Otras amenazas que se presentan en menores porcentajes son la quitridiomicosis, degradación del hábitat y sobrexplotación. Se recomienda el fomento de áreas de conservación privadas para las especies de anfibios andinos presentes en Perú con categorías de amenaza, endémicas y que no están dentro del SINANPE.

  17. LA EVIDENCIALIDAD EN EL CASTELLANO ANDINO NARIÑENSE EVIDENTIALITY IN THE CASTILIAN SPANISH OF THE ANDEAN REGION OF NARIÑO

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    Ómar Andrés Portilla Melo

    2010-12-01

    Full Text Available Resumen En este texto se hace una descripción de un fenómeno de transferencia semánticopragmática a nivel modal del quechua al castellano andino de la región nariñense. El trabajo está organizado de la siguiente manera: en primer lugar, se introduce el concepto de evidencialidad; después, se hace una breve descripción histórica del quechua y una introducción a su tipología, y, finalmente, se explica la manera en que el contacto lingüístico quechua-español le dio al castellano andino nariñense los aspectos que lo caracterizan. La metodología usada fue la entrevista etnográfica. Como resultado se obtuvo que la influencia del quechua en el castellano del departamento de Nariño no se dio solamente a nivel léxico o sintáctico, sino que alcanzó niveles más profundos.Abstract The article describes a phenomenon of semantic-pragmatic transfer at the modal level from Quechua into the Castilian Spanish of the Andean region of Nariño. The article is organized as follows: first it introduces the concept of evidentiality, next it provides a brief historical description of Quechua and an introduction to its typology, and, finally, it explains how the linguistic contact between Quechua and Spanish gave the Castilian Spanish of the Andean region of Nariño its characteristic aspects. The methodology used was the ethnographic interview. The results obtained show that the influence of Quechua on the Castilian Spanish of the department of Nariño occurred not only at the lexical or syntactic levels, but also at deeper levels.

  18. Análisis geográfico y conservación del zorro andino Lycalopex culpaeus (Mammalia, Canidae en Colombia

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    Elkin A. Noguera-Urbano

    2016-01-01

    Full Text Available RESUMEN El zorro andino Lycalopex culpaeus (Molina, 1782 ha sido incluido en la categoría de amenaza Vulnerable en Colombia, sin embargo, no se han realizado estudios que validen la inclusión de la especie en dicha categoría a nivel nacional. En el presente trabajo se delimitó el área de distribución potencial (DP de la especie en Colombia y se discute su categoría de amenaza. Para ello se usó modelamiento de nicho ecológico en el algoritmo de Máxima Entropía (Maxent. Con procesamiento en Sistemas de Información Geográfico resaltamos las áreas donde la especie se encuentra protegida. Los resultados muestran que el zorro andino se podría encontrar desde los departamentos de Nariño hasta el Huila (8,877 km2, y el 19.6% (1,742 km2 del área de distribución potencial de la especie se encuentra dentro del Sistema Nacional de Áreas Protegidas de Colombia. A pesar, que la especie es considerada Vulnerable en Colombia, se encontró que no cumple con los criterios para su asignación en dicha categoría debido a la ausencia de evidencias tangibles que sustenten una reducción poblacional, disminución del área de ocupación o la calidad del hábitat en la década previa a la asignación de la categoría de amenaza. Teniendo en cuenta los registros verificables e información sobre el área de distribución de la especie en Colombia, se propone una reasignación de la categoría de amenaza nacional a Casi Amenazada (NT.

  19. The Red de Monitoreo de BosquesAndinos: A communication platform for science and policy in the Andean countries

    Science.gov (United States)

    Baez, S.; Cuesta, F. X.; Malizia, A.; Carilla, J.; Bustamante, M.; Yepes, A.

    2013-05-01

    A workshop held in October 2012 in Lima, Peru, brought together more than 40 scientists and policy makers working in Andean forest ecosystems, one of the richer and most threatened ecosystems of the world. Among the various results of the workshop, there is the formation of the network "Red de Bosques Andinos". The goals of the network include to stimulate scientific research in Andean forest ecosystems by promoting collaboration among scientists, and to serve as a platform to facilitate applied research and communication between scientists and policy makers. Current members of the network include scientists of Argentina, Colombia, Ecuador, Germany, Peru, USA, and representatives of Ministries of Environment and the National Climate Change Adaptation Programs of Colombia, Bolivia, Ecuador, and Peru. The network has started to work in two critical documents for the region. The first one is an extended protocol to monitor diversity and carbon in Andean forests. This protocol, partly based on unpublished efforts, has been developed by the Instituto de Ecología Regional, Universidad de Tucuman, Argentina, and has been revised and improved by experts working in the Andes. The document describe methods to document ecological changes that take place over mid- and long periods of time. It focuses on monitoring changes on the diversity and growth of trees, shrubs and lianas, cover of herbaceous species, and carbon content in forests. This extended protocol will be a useful tool for students and researchers interested in conducting long-term ecological research. Moreover, the use of this tool will produce standardized data needed to understand ecological processes that take place at large spatial scales. The document will be freely available at www.condesan.org. The second document consists of an analysis of the dynamics of trees and carbon in the Andean region. The members of the network have contributed with data of more than 70 permanent forest plots located from

  20. An Efficient Inverse Kinematic Algorithm for a PUMA560-Structured Robot Manipulator

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    Huashan Liu

    2013-05-01

    Full Text Available This paper presents an efficient inverse kinematics (IK approach which features fast computing performance for a PUMA560‐structured robot manipulator. By properties of the orthogonal matrix and block matrix, the complex IK matrix equations are transformed into eight pure algebraic equations that contain the six unknown joint angle variables, which makes the solving compact without computing the reverses of the 4×4 homogeneous transformation matrices. Moreover, the appropriate combination of related equations ensures that the solutions are free of extraneous roots in the solving process, and the wrist singularity problem of the robot is also addressed. Finally, a case study is given to show the effectiveness of the proposed algorithm.

  1. Esophageal hiatal hernia in three exotic felines--Lynx lynx, Puma concolore, Panthera leo.

    Science.gov (United States)

    Hettlich, Bianca F; Hobson, H Phil; Ducoté, Julie; Fossum, Theresa W; Johnson, James H

    2010-03-01

    Hiatal hernia was diagnosed in three exotic felines-lynx (Lynx lynx), cougar (Puma concolore), and lion (Panthera leo). All cats had a history of anorexia. Thoracic and abdominal radiographs showed evidence of a soft tissue mass within the caudal mediastinum suggestive of a hiatal hernia in all animals. A barium esophagram was performed in one case. All animals underwent thoracic or abdominal surgery for hernia reduction. Surgical procedures included: intercostal thoracotomy with herniorrhaphy and esophagopexy (lynx and cougar), and incisional gastropexy (lion). Concurrent surgical procedures performed were gastrotomy for gastric foreign body removal and jejunostomy tube placement. Clinical signs related to the hiatal hernia disappeared after surgery and recurrence of signs was not reported for the time of follow-up.

  2. PUMA PUblication MAnagement: un network di Archivi Istituzionali CNR, ad accesso aperto, nella prospettiva di una infrastruttura per la Ricerca in Europa

    OpenAIRE

    Biagioni, Stefania

    2008-01-01

    The Publication Management System (PUMA) is one of the major software component made available by the CNR Portal Of Libraries And Repositories (POLAR) that provides CNR librarians and researchers with services for managing libraries and accessing information. PUMA also constitutes the first step towards creating the Italian network of CNR Institutional repositories, looking at the DRIVER vision, i.e., building an infrastructure that allows European Research Institutions to share content and f...

  3. Inhibition of AKT/FoxO3a signaling induced PUMA expression in response to p53-independent cytotoxic effects of H1: A derivative of tetrandrine.

    Science.gov (United States)

    Zhang, Yin-Xu; Liu, Xiao-Mei; Wang, Jing; Li, Jun; Liu, Ying; Zhang, Hua; Yu, Xue-Wen; Wei, Ning

    2015-01-01

    PUMA (p53 unregulated modulator of apoptosis), a BH3-only Bcl-2 family member, can be induced by p53-dependent and p53-independent manners. It plays an important role as regulator of cellular apoptosis. Herein, we evaluate the effects of H1 (a derivative of tetrandrine) on induction of PUMA and underlie its potential mechanism in p53-independent cytotoxic response. Anti-proliferative activity and evidently cytotoxic activity of H1 were observed in wild-type and p53 null cells. Further studies demonstrated that H1 resulted in an increase of cleaved PARP, decease of survivin and elevation of p-H2AX. What is more, H1 significantly induced PUMA expression in a concentration- and time-dependent manner and caused an increase of Bax/Bcl-2 ratio in p53 null cells. Of note, knockdown of PUMA attenuated cytotoxic activity of H1. Further studies demonstrated that inhibition of AKT/FoxO3a signaling contributed to H1-mediated PUMA induction. Targeted suppression of AKT/FoxO3a signaling by siRNA could overcome H1-mediated PUMA induction. In addition, H1 significantly suppressed NF-κB activity and caused an increase of early apoptotic and late apoptotic cells, and elevated caspase-3 activity. Taken together, we found that inhibition of AKT/FoxO3a signaling may contribute to H1-mediated PUMA induction, suggesting that inhibition of AKT/FoxO3a signaling result in PUMA expression in response to p53-independent cytotoxic effects of H1.

  4. Maximal killing of lymphoma cells by DNA damage-inducing therapy requires not only the p53 targets Puma and Noxa, but also Bim.

    Science.gov (United States)

    Happo, Lina; Cragg, Mark S; Phipson, Belinda; Haga, Jon M; Jansen, Elisa S; Herold, Marco J; Dewson, Grant; Michalak, Ewa M; Vandenberg, Cassandra J; Smyth, Gordon K; Strasser, Andreas; Cory, Suzanne; Scott, Clare L

    2010-12-09

    DNA-damaging chemotherapy is the backbone of cancer treatment, although it is not clear how such treatments kill tumor cells. In nontransformed lymphoid cells, the combined loss of 2 proapoptotic p53 target genes, Puma and Noxa, induces as much resistance to DNA damage as loss of p53 itself. In Eμ-Myc lymphomas, however, lack of both Puma and Noxa resulted in no greater drug resistance than lack of Puma alone. A third B-cell lymphoma-2 homology domain (BH)3-only gene, Bim, although not a direct p53 target, was up-regulated in Eμ-Myc lymphomas incurring DNA damage, and knockdown of Bim levels markedly increased the drug resistance of Eμ-Myc/Puma(-/-)Noxa(-/-) lymphomas both in vitro and in vivo. Remarkably, c-MYC-driven lymphoma cell lines from Noxa(-/-)Puma(-/-)Bim(-/-) mice were as resistant as those lacking p53. Thus, the combinatorial action of Puma, Noxa, and Bim is critical for optimal apoptotic responses of lymphoma cells to 2 commonly used DNA-damaging chemotherapeutic agents, identifying Bim as an additional biomarker for treatment outcome in the clinic.

  5. The Evaluation and Comparison of Transcriptionally Targeted Noxa and Puma Killer Genes to Initiate Apoptosis Under Cancer-Specific Promoter CXCR1 in Hepatocarcinoma Gene Therapy

    Directory of Open Access Journals (Sweden)

    Khoshtinat Nikkhoi

    2016-09-01

    Full Text Available Background Cancerous cells proliferate as fast as possible without a proper surveillance system. This rapid cell division leads to enormous mutation rates, which help a tumor establish. Objectives This study evaluated the potential of inducing apoptosis using Noxa and Puma in a hepatocarcinoma cell line. Methods The current study generated two recombinant lentiviruses, pLEX-GCN and pLEX-GCP, bearing Noxa and Puma, respectively. Transduction of both genes to hepatocarcinoma (HepG2 was verified using fluorescent microscopic analysis, western blotting, and quantitative real-time polymerase chain reaction (PCR. To evaluate the potential of Noxa and Puma to initiate apoptosis, a caspase-9 real-time, MTT assay, and a 4’, 6-diamidino-2-phenylindole (DAPI reagent were performed to stain apoptotic cells. Results The data verified successful transduction to HepG2 and HEK293T. Higher relative expression of Noxa and Puma rather than the untransduced cell line showed these genes are expressed more in HepG2 in comparison to HEK293T. The results of the real-time PCR, MTT assay, and DAPI reagent illustrated that higher cells initiated apoptosis following Puma transduction rather than Noxa. Conclusions In this approach, the suicide gene was transferred to transformed cells and ignited apoptosis to exterminate them. Puma is a more potent killer gene and has higher capabilities to start intrinsic apoptosis pathway.

  6. Puma and p21 represent cooperating checkpoints limiting self-renewal and chromosomal instability of somatic stem cells in response to telomere dysfunction.

    Science.gov (United States)

    Sperka, Tobias; Song, Zhangfa; Morita, Yohei; Nalapareddy, Kodandaramireddy; Guachalla, Luis Miguel; Lechel, André; Begus-Nahrmann, Yvonne; Burkhalter, Martin D; Mach, Monika; Schlaudraff, Falk; Liss, Birgit; Ju, Zhenyu; Speicher, Michael R; Rudolph, K Lenhard

    2011-12-04

    The tumour suppressor p53 activates Puma-dependent apoptosis and p21-dependent cell-cycle arrest in response to DNA damage. Deletion of p21 improved stem-cell function and organ maintenance in progeroid mice with dysfunctional telomeres, but the function of Puma has not been investigated in this context. Here we show that deletion of Puma improves stem- and progenitor-cell function, organ maintenance and lifespan of telomere-dysfunctional mice. Puma deletion impairs the clearance of stem and progenitor cells that have accumulated DNA damage as a consequence of critically short telomeres. However, further accumulation of DNA damage in these rescued progenitor cells leads to increasing activation of p21. RNA interference experiments show that upregulation of p21 limits proliferation and evolution of chromosomal imbalances of Puma-deficient stem and progenitor cells with dysfunctional telomeres. These results provide experimental evidence that p53-dependent apoptosis and cell-cycle arrest act in cooperating checkpoints limiting tissue maintenance and evolution of chromosomal instability at stem- and progenitor-cell levels in response to telomere dysfunction. Selective inhibition of Puma-dependent apoptosis can result in temporary improvements in maintenance of telomere-dysfunctional organs.

  7. Phylogenetically Distant Viruses Use the Same BH3-Only Protein Puma to Trigger Bax/Bak-Dependent Apoptosis of Infected Mouse and Human Cells.

    Science.gov (United States)

    Papaianni, Emanuela; El Maadidi, Souhayla; Schejtman, Andrea; Neumann, Simon; Maurer, Ulrich; Marino-Merlo, Francesca; Mastino, Antonio; Borner, Christoph

    2015-01-01

    Viruses can trigger apoptosis of infected host cells if not counteracted by cellular or viral anti-apoptotic proteins. These protective proteins either inhibit the activation of caspases or they act as Bcl-2 homologs to prevent Bax/Bak-mediated outer mitochondrial membrane permeabilization (MOMP). The exact mechanism by which viruses trigger MOMP has however remained enigmatic. Here we use two distinct types of viruses, a double stranded DNA virus, herpes simplex virus-1 (HSV-1) and a positive sense, single stranded RNA virus, Semliki Forest virus (SFV) to show that the BH3-only protein Puma is the major mediator of virus-induced Bax/Bak activation and MOMP induction. Indeed, when Puma was genetically deleted or downregulated by shRNA, mouse embryonic fibroblasts and IL-3-dependent monocytes as well as human colon carcinoma cells were as resistant to virus-induced apoptosis as their Bax/Bak double deficient counterparts (Bax/Bak-/-). Puma protein expression started to augment after 2 h postinfection with both viruses. Puma mRNA levels increased as well, but this occurred after apoptosis initiation (MOMP) because it was blocked in cells lacking Bax/Bak or overexpressing Bcl-xL. Moreover, none of the classical Puma transcription factors such as p53, p73 or p65 NFκB were involved in HSV-1-induced apoptosis. Our data suggest that viruses use a Puma protein-dependent mechanism to trigger MOMP and apoptosis in host cells.

  8. Validación y aplicación de la prueba ELISA para medir cortisol fecal en jaguar (Panthera onca y puma (Puma concolor durante un programa de enriquecimiento ambiental en el Zoológico Jaime Duque

    Directory of Open Access Journals (Sweden)

    Catalina Rodríguez Álvarez

    2005-12-01

    Full Text Available El enriquecimiento ambiental busca aumentar el bienestar de los animales cautivos mediante la provisión de estímulos que motiven la realización de comportamientos típicos de la especie. A las poblaciones de jaguares (Panthera onca y pumas (Puma concolor presentes en el Zoológico Jaime Duque se les aplicó un programa de enriquecimiento ambiental y con el fin de probar si su bienestar aumentaba, se realizaron mediciones de cortisol en materia fecal mediante la prueba de ELISA, para lo cual hubo necesidad de validar la técnica para cada especie. La prueba utilizada resultó ser válida para ambas especies; sin embargo, los niveles de cortisol se ven influenciados por múltiples variables y no se hizo evidente la reducción de los niveles del mismo con el enriquecimiento ambiental.

  9. Genetic variability of Herpailurus yagouaroundi, Puma concolor and Panthera onca (Mammalia, Felidae studied using Felis catus microsatellites

    Directory of Open Access Journals (Sweden)

    Vanessa Roma Moreno

    2006-01-01

    Full Text Available We used four microsatellite loci (Fca08, Fca45, Fca77 and Fca96 from the domestic cat, Felis catus, to investigate genetic variability in specimens of Herpailurus yagouaroundi (jaguarundi, otter cat, eyra, Puma concolor (cougar, mountain lion, puma and Panthera onca (jaguar held in various Brazilian zoos. Samples of DNA from the cats were PCR amplified and then sequenced before being analyzed using the CERVUS program. Our results show a mean polymorphic information content (PIC of 0.83 for H. yagouaroundi, 0.66 for P. concolor and 0.69 for P. onca and a mean of 10.3 alleles for the Fca08 locus, 5.3 for Fca 45, 9 for Fca 77 and 14 for Fca 96. These results indicate a relatively high level of genetic diversity for the specimens studied.

  10. A new metastrongyloidean species (Nematoda) parasitizing pulmonary arteries of Puma (Herpailurus) yagouaroundi (É. Geoffroy, 1803) (Carnivora: Felidae) from Brazil.

    Science.gov (United States)

    Vieira, Fabiano M; Muniz-Pereira, Luís C; de Souza Lima, Sueli; Neto, Antonio H A Moraes; Guimarães, Erick V; Luque, José L

    2013-04-01

    Angiostrongylus felineus n. sp. (Nematoda, Metastrongyloidea), parasitic in Puma (Herpailurus) yagouaroundi (É. Geoffroy, 1803) (Carnivora, Felidae) from the municipality of Juiz de Fora, Minas Gerais state, Brazil, is described and illustrated herein. Angiostrongylus felineus n. sp. differs from all congeneric species by having the anterior extremity with accentuated cuticular expansion and by smaller size of spicules. This study describes for the first time a species of Angiostrongylus in a wild Felidae in Brazil.

  11. A critical role of PUMA in maintenance of genomic integrity of murine spermatogonial stem cell precursors after genotoxic stress

    Institute of Scientific and Technical Information of China (English)

    Anne Forand; J Bernardino-Sgherri

    2009-01-01

    Neonatal gonocytes are precursors of spermatogoniai stem cells. Preserving their integrity by elimination of dam-aged germ cells may be crucial to avoid the transmission of genetic alterations to progeny. Using T-irradiation, we investigated by immunohistochemistry, flow cytometry and real-time PCR components of the death machinery in neonatal gonocytes. Their death was correlated with caspase 3 activation but not with AIF translocation into the nu-cleus. The in vivo contribution of both the extrinsic and the intrinsic pathways was then investigated. We focused on the roles of TRAIL/Death Receptor 5 (DR5) and PUMA. Our results were validated using knockout mice. Whereas DR5 expression was upregulated at the cell surface after radiation, caspase 8 was not activated. However, we detected caspase 9 cleavage associated with cytochrome c release. In mice deficient for PUMA, radiation-induced gonocyte apoptosis was reduced, whereas invalidation of TRAIL had no effect. Overall, our results show that genotoxic stress-induced apoptosis of gonocytes is caspase-dependent and involves almost exclusively the intrinsic pathway. Further-more, PUMA plays a critical role in the maintenance of genomic integrity of spermatogonial stem cell precursors.

  12. Relación del gradiente interior-borde de fragmentos de bosque andino sobre la comunidad de murciélagos en Encino (Santander, Colombia

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    Otálora Ardila Aída

    2005-07-01

    Full Text Available En este trabajo se describe la configuración espacial del paisaje del municipio de Encino y la presencia de efecto de borde sobre la comunidad de murciélagos en fragmentos de bosque subandino inmersos en una matriz agrícola. Se analizó información cartográfica existente del municipio en el software ArcView 3.2 para determinar propiedades del paisaje como área total, número y densidad de parches y se estimaron índices que describen el grado de fragmentación para bosques andinos y subandinos y algunos atributos de los fragmentos como área y forma. Hay 53 fragmentos de bosque subandino y 111 de andino (164 fragmentos totales que cubren 152,5 km2 del
    área total y tienen una densidad de 0,39 parches/ha. Los índices calculados revelan que los bosques andinos tienen un menor proceso de fragmentación que los subandinos representado en fragmentos más grandes y más cercanos. El paisaje ha sido muy transformado pues el 90,4% de los fragmentos son <50 ha y el área transformada alcanza un 53,3% del área del municipio. Se sugiere que el bosque subandino está críticamente amenazado y que deben proponerse planes de conservación para los bosques fragmentados de Encino pues albergan parte de la biodiversidad original y pueden funcionar como refugios de diversidad. En cuanto a la comunidad de murciélagos asociada a este mosaico, se realizó un muestreo con 16 redes de niebla durante 106 noches, cubriendo un gradiente matriz-interior de bosque en cuatro fragmentos de bosque subandino (10-50 ha, para describir los cambios en la composición, diversidad de especies y de gremios en la comunidad de murciélagos asociados a este gradiente. Se capturaron 709 individuos
    de 24 especies pertenecientes a las familias Phyllostomidae y Vespertilionidae. La riqueza específica, la diversidad de especies y la diversidad de gremios alcanzaron los mayores valores en el borde y en la matriz. Se sugiere un efecto de borde indirecto en donde la riqueza

  13. International BMI-for-age references underestimate thinness and overestimate overweigth and obesity in Bolivian adolescents Las referencias internacionales de IMC por edad subestiman delgadez y sobreestiman sobrepeso y obesidad en adolescentes bolivianos

    Directory of Open Access Journals (Sweden)

    A. Baya Botti

    2010-06-01

    Full Text Available Background: Since no growth standards for adolescents exist and a single reference applicable everywhere is still in debate, it is recognized that the best reference should be derived from the growth pattern of the healthy population that will use it. In 2007 a study developed references for body mass index for 12th to 18th y Bolivian school adolescent (BAP. Objectives: To compare nutritional status outcomes applying BMI references from the BAP, the Center for Disease Control and Prevention CDC 2000, the International Task Force (IOTF, and the 2007 WHO, to determine appropriateness of use in Bolivian adolescents. Subjects/methods: References were applied in 3306 adolescents, 45.0% male, 55% female, 12th to 18th y selected from a nationally representative sample. Results: Main findings reveal that the CDC and the 2007 WHO underestimate underweight (p Introducción: Puesto que no existen estándares de crecimiento para adolescentes y la aplicación de una sola referencia a nivel mundial es aún debatible, se reconoce que la mejor referencia es aquella derivada del patrón de crecimiento de la población saludable en la que será posteriormente utilizada. Por ello en 2007 se efectuó un estudio para desarrollar la Referencia Boliviana de IMC poredad (BAP para adolescentes entre 12 a 18 años. Objetivos: Comparar la clasificación de adolescentes por categorías nutricionales, aplicando las referencias de IMC boliviano (BAP, la referencia CDC 2000, los puntosde corte de IOTF y de la OMS, 2007. Métodos y población: Las referencias se aplicaron a los datos medidos de peso y talla de 3.306 adolescentes, 45% varones y 55% mujeres seleccionados de una muestrarepresentativa de la población boliviana. Resultados: Se observa que CDC y OMS 2007 subestiman la prevalencia de delgadez (P < 0,001 mientras que las tres referencias internacionales sobreestiman el sobrepeso(p < 0,001 con variaciones por edad y género. Conclusión: Se recomienda al personal de

  14. Combined loss of PUMA and p21 accelerates c-MYC-driven lymphoma development considerably less than loss of one allele of p53.

    Science.gov (United States)

    Valente, L J; Grabow, S; Vandenberg, C J; Strasser, A; Janic, A

    2016-07-21

    The tumor suppressor p53 is mutated in ~50% of human cancers. P53 is activated by a range of stimuli and regulates several cellular processes, including apoptotic cell death, cell cycle arrest, senescence and DNA repair. P53 induces apoptosis via transcriptional induction of the BH3-only proteins PUMA (p53-upregulated modulator of apoptosis) and NOXA, and cell cycle arrest via p21. Induction of these processes was proposed to be critical for p53-mediated tumor suppression. It is therefore surprising that mice lacking PUMA, NOXA and p21, as well as mice bearing mutations in p53 that impair the transcriptional activation of these genes, are not tumor prone, unlike mice lacking p53 function, which spontaneously develop tumors with 100% incidence. These p53 target genes and the processes they regulate may, however, impact differently on tumor development depending on the oncogenic drivers. For example, loss of PUMA enhances c-MYC-driven lymphoma development in mice, but, interestingly, the acceleration was less impressive compared with that caused by the loss of even a single p53 allele. Different studies have reported that loss of p21 can accelerate, delay or have no impact on tumorigenesis. In an attempt to resolve this controversy, we examined whether loss of p21-mediated cell cycle arrest cooperates with PUMA deficiency in accelerating lymphoma development in Eμ-Myc mice (overexpressing c-MYC in B-lymphoid cells). We found that Eμ-Myc mice lacking both p21 and PUMA (Eμ-Myc;Puma(-/-);p21(-/-)) developed lymphoma at a rate comparable to Eμ-Myc;Puma(-/-) animals, notably with considerably longer latency than Eμ-Myc;p53(+/-)mice. Loss of p21 had no impact on the numbers, cycling or survival of pre-leukemic Eμ-Myc B-lymphoid cells, even when PUMA was lost concomitantly. These results demonstrate that even in the context of deregulated c-MYC expression, p53 must suppress tumor development by activating processes apart from, or in addition to, PUMA

  15. Birth timing for mountain lions (Puma concolor); testing the prey availability hypothesis.

    Science.gov (United States)

    Jansen, Brian D; Jenks, Jonathan A

    2012-01-01

    We investigated potential advantages in birth timing for mountain lion (Puma concolor) cubs. We examined cub body mass, survival, and age of natal dispersal in relation to specific timing of birth. We also investigated the role of maternal age relative to timing of births. We captured mountain lion cubs while in the natal den to determine birth date, which allowed for precise estimates of the population birth pulse and age of natal dispersal. A birth pulse occurred during June-August. Body mass of cubs was related to litter size and timing of birth; heaviest cubs occurred in litters of 2, and those born after 1 July. Cubs born within pulse months exhibited similar survival to those born out of the pulse. We found that cubs born April-June dispersed at younger ages than those born after 1 July. There was less variation in birth timing for 1(st) litters of females than older females. We hypothesize that cubs born after the peak in births of neonate prey are advantaged by the abundance of vulnerable prey and those cubs and mothers realize an evolutionary advantage.

  16. A mono-dimensional nuclear fuel performance analysis code, PUMA, development from a coupled approach

    Energy Technology Data Exchange (ETDEWEB)

    Cheon, J. S.; Lee, B. O.; Lee, C. B. [Korea Atomic Energy Research Institute, 989-111 Daedeok-daero, Yuseong, Daejeon, 305-353 (Korea, Republic of); Yacout, A. M. [Argonne National Laboratory, 9700 South Cass Avenue, Argonne, IL 60439 (United States)

    2013-07-01

    Multidimensional-multi-physical phenomena in nuclear fuels are treated as a set of mono-dimensional-coupled problems which encompass heat, displacement, fuel constituent redistribution, and fission gas release. Rather than uncoupling these coupled equations as in conventional fuel performance analysis codes, efforts are put into to obtain fully coupled solutions by relying on the recent advances of numerical analysis. Through this approach, a new SFR metal fuel performance analysis code, called PUMA (Performance of Uranium Metal fuel rod Analysis code) is under development. Although coupling between temperature and fuel constituent was made easily, the coupling between the mechanical equilibrium equation and a set of stiff kinetics equations for fission gas release is accomplished by introducing one-level Newton scheme through backward differentiation formula. Displacement equations from 1D finite element formulation of the mechanical equilibrium equation are solved simultaneously with stress equation, creep equation, swelling equation, and FGR equations. Calculations was made successfully such that the swelling and the hydrostatic pressure are interrelated each other. (authors)

  17. Feline infectious peritonitis in a mountain lion (Puma concolor), California, USA.

    Science.gov (United States)

    Stephenson, Nicole; Swift, Pamela; Moeller, Robert B; Worth, S Joy; Foley, Janet

    2013-04-01

    Feline infectious peritonitis (FIP) is a fatal immune-mediated vasculitis of felids caused by a mutant form of a common feline enteric virus, feline enteric coronavirus. The virus can attack many organ systems and causes a broad range of signs, commonly including weight loss and fever. Regardless of presentation, FIP is ultimately fatal and often presents a diagnostic challenge. In May 2010, a malnourished young adult male mountain lion (Puma concolor) from Kern County, California, USA was euthanized because of concern for public safety, and a postmortem examination was performed. Gross necropsy and histopathologic examination revealed necrotizing, multifocal myocarditis; necrotizing, neutrophilic, and histiocytic myositis and vasculitis of the tunica muscularis layer of the small and large intestines; and embolic, multifocal, interstitial pneumonia. Feline coronavirus antigen was detected in both the heart and intestinal tissue by immunohistochemistry. A PCR for coronavirus performed on kidney tissue was positive, confirming a diagnosis of FIP. Although coronavirus infection has been documented in mountain lions by serology, this is the first confirmed report of FIP.

  18. Therapeutic Response to Non-genotoxic Activation of p53 by Nutlin3a Is Driven by PUMA-Mediated Apoptosis in Lymphoma Cells

    Directory of Open Access Journals (Sweden)

    Liz J. Valente

    2016-03-01

    Full Text Available Nutlin3a is a small-molecule antagonist of MDM2 that promotes non-genotoxic activation of p53 through p53 protein stabilization and transactivation of p53 target genes. Nutlin3a is the forerunner of a class of cancer therapeutics that have reached clinical trials. Using transgenic and gene-targeted mouse models lacking the critical p53 target genes, p21, Puma, and Noxa, we found that only loss of PUMA conferred profound protection against Nutlin3a-induced killing in both non-transformed lymphoid cells and Eμ-Myc lymphomas in vitro and in vivo. CRISPR/Cas9-mediated targeting of the PUMA gene rendered human hematopoietic cancer cell lines markedly resistant to Nutlin3a-induced cell death. These results demonstrate that PUMA-mediated apoptosis, but not p21-mediated cell-cycle arrest or senescence, is a critical determinant of the therapeutic response to non-genotoxic p53 activation by Nutlin3a. Importantly, in human cancer, PUMA expression may predict patient responses to treatment with MDM2 antagonists.

  19. Therapeutic Response to Non-genotoxic Activation of p53 by Nutlin3a Is Driven by PUMA-Mediated Apoptosis in Lymphoma Cells.

    Science.gov (United States)

    Valente, Liz J; Aubrey, Brandon J; Herold, Marco J; Kelly, Gemma L; Happo, Lina; Scott, Clare L; Newbold, Andrea; Johnstone, Ricky W; Huang, David C S; Vassilev, Lyubomir T; Strasser, Andreas

    2016-03-01

    Nutlin3a is a small-molecule antagonist of MDM2 that promotes non-genotoxic activation of p53 through p53 protein stabilization and transactivation of p53 target genes. Nutlin3a is the forerunner of a class of cancer therapeutics that have reached clinical trials. Using transgenic and gene-targeted mouse models lacking the critical p53 target genes, p21, Puma, and Noxa, we found that only loss of PUMA conferred profound protection against Nutlin3a-induced killing in both non-transformed lymphoid cells and Eμ-Myc lymphomas in vitro and in vivo. CRISPR/Cas9-mediated targeting of the PUMA gene rendered human hematopoietic cancer cell lines markedly resistant to Nutlin3a-induced cell death. These results demonstrate that PUMA-mediated apoptosis, but not p21-mediated cell-cycle arrest or senescence, is a critical determinant of the therapeutic response to non-genotoxic p53 activation by Nutlin3a. Importantly, in human cancer, PUMA expression may predict patient responses to treatment with MDM2 antagonists.

  20. El frente de corrimiento andino al nivel de los cerros Penitentes y Visera (alta Cordillera de Mendoza: aspectos cronológicos y cartográficos

    Directory of Open Access Journals (Sweden)

    Jean-Claude Vicente

    2009-10-01

    Full Text Available El hallazgo de una iportante fauna de invertebrados marinos del Tithoniano superior a espaldas del cerro Penitentes en la secuencia carbonática basal de la primera escama del frente de corrimiento andino, confirma la lógica tectónica de un despegue de esta unidad a nivel del Yeso Principal y lleva a invalidar ciertas correlaciones litoestratigráficas y bosquejos paleogeográficos asignados a la Formación La Manga. Por otro lado, el análisis de algunos perfiles claves en torno al cerro Penitentes entre la quebrada Vargas y el cerro Visera permite presentar una reinterpretación tectónica del sector. Esta se sustenta en el reconocimiento y la diferenciación de las unidades terciarias básicas representadas por los Conglomerados Penitentes (Mioceno inferior y los Aglomerados Santa María (Mioceno superior en una relación muy similar a la descrita anteriormente al norte de Puente del Inca. El estudio permite seguir las interferencias, en un contexto polifásico, entre las reactivaciones del corrimiento Penitentes de piel fina y un cabalgamiento frontal de piel gruesa que repite la secuencia terciaria. La discordancia entre las secuencias terciarias y el emplazamiento de una klippe de Malm sobre los Conglomerados Penitentes del sinclinal del cerro Visera, conduce a otorgar cierta importancia a la fase del final del Mioceno inferior.

  1. Nuevas evidencias de la presencia del Oso Andino (Tremarctos ornatus en las Yungas de Puno, el registro más austral de Perú

    Directory of Open Access Journals (Sweden)

    Gisella Márquez

    2011-05-01

    Full Text Available El oso de anteojos Tremarctos ornatus es el único representante de la familia Ursidae en Suramérica. La población más grande y de distribución continua del oso andino en el Perú, se localiza en la ladera oriental de la cordillera oriental, incluyendo al departamento de Puno, donde casi todos los registros de esta especie provienen solamente de encuestas. Se describe aquí dos registros indirectos de la presencia del oso en el departamento de Puno, obtenidos en agosto del año 2009. El primer registro corresponde a una feca, con semillas perteneciente a la familia Lauraceae, encontrada en Yanacocha; y el segundo registro corresponde a unas marcas de garras del oso hallado en Challohuma. Este último representa el registro más austral con- firmado para la distribución del oso de anteojos en el Perú. Nuestros registros evidencian una continuidad en la distribución de esta especie en la vertiente oriental de la cordillera andina, desde el sur del Perú hasta el noroeste de Bolivia. Estos registros se hallan también muy próximos al Parque Nacional Bahuaja Sonene (PNBS, uno de los corredores de conservación más importantes en el mundo, y muestran la importancia de establecer estrategias de conservación tanto dentro como fuera de la zona de amortiguamiento del PNBS.

  2. La transformación del Estado en América Latina: una perspectiva económica desde los países andinos

    Directory of Open Access Journals (Sweden)

    Edgard Moncayo Jiménez

    2006-01-01

    Full Text Available A juicio de muchos analistas, el Estadonación —piedra angular de la organización política del mundo desde la suscripción del Tratado de Wesfalia, en 1968— está experimentando una profunda crisis. En esta visión, el poder efectivo de dicha entidad política se está desbordando en tres direcciones: hacia arriba, por la globalización y la supranacionalización; horizontalmente, por el desmonte del Estado de bienestar, la privatización y la desregulación; y hacia abajo, por la descentralización y la devolución de autonomías a las polis regionales y locales. La acción conjunta de estas tendencias estaría determinando el vaciamiento (hollowing out del Estado-nación, en la medida en que muchas de sus competencias tradicionales se están transfiriendo hacia arriba, a las instituciones internacionales y supranacionales; hacia los lados, al mercado, las empresas privadas y las transnacionales; y hacia abajo, a las entidades de orden subnacional. En este artículo analizaremos el impacto de tales tendencias en los países andinos

  3. El zorro Andino, P seudalopes P seudalopes P culpaeus , un nuevo huésped para Corynosoma obtuscens (Acanthocephala en el Perú*

    Directory of Open Access Journals (Sweden)

    Manuel Tantaleán

    2013-04-01

    Full Text Available Se describe la presencia de Corynosoma obtuscens Lincicome, 1943 (Acanthocephala, Polymor- Corynosoma obtuscens L i n c i c o m e , 1 9 4 3 ( A c a n t h o c e p h a l a , P o l y m o r - Corynosoma obtuscens phidae en el intestino delgado del zorro andino Pseudalopex culpaeus (Molina, 1782 procedente Pseudalopex culpaeus ( M o l i n a , 1 7 8 2 p r o c e d e n t e Pseudalopex culpaeus de San Juan de Marcona, Departamento de Ica, Perú. Esta es la segunda vez que se registra la infección natural de un mamífero terrestre por C. obtuscens en el Perú, por lo que se considera C. obtuscens en el Perú, por lo que se considera C. obtuscens a P. culpaeus como huésped defi nitivo accidental P. culpaeus como huésped defi nitivo accidental P. culpaeus ; además se comenta la posibilidad de que éste acantocefalo infecte al hombre.

  4. Su majestad quiere saber. Información oficial y reformismo borbónico: el mundo andino bajo la mirada de la ilustración

    Directory of Open Access Journals (Sweden)

    Juan Marchena Fernández

    2005-11-01

    Full Text Available Estudio dedicado a analizar el proceso de recolección de información burocrática que la Corona española requirió de sus posesiones americanas, durante las últimas décadas del siglo XVIII. La monarquía española demandó información sobre una variedad de aspectos que incluían producción, población, rentas, impuestos, doctrinas, costumbres, etc. El estudio escudriña, de manera específica, las vicisitudes que marcaron el cumplimiento de esta disposición real en el espacio andino. Se analizan las mediaciones sociales, políticas y culturales locales que estuvieron presentes en el proceso de recolección de la información. Por un lado, se subraya la distancia existente entre las premisas reformistas ilustradas que animaban los intereses de la monarquía española, y por otro, las maneras cómo reaccionaron la burocracia colonial y las sociedades andinas locales.

  5. La participación política de bolivianos y paraguayos residentes de villas en la ciudad de Buenos Aires: una aproximación desde la lucha por la vivienda

    Directory of Open Access Journals (Sweden)

    Carla Gallinati

    Full Text Available Resumen Este artículo se dedica en analizar la participación política de los migrantes bolivianos y paraguayos que, al lado de la población nacional, desarrollan un proceso de reclamo por la urbanización y por la vivienda digna en las villas de la Ciudad de Buenos Aires. Presentaré parte de la cartografía de discusiones de un grupo específico de residentes de villas que, independiente del origen nacional, compartían problemáticas comunes y reivindicaron ser reconocidos como vecinos de la ciudad y militantes en lucha por la vivienda. La propuesta es poner en foco dos aspectos poco observados por los estudios del campo migratorio: a la centralidad de la escala urbana para el estudio del fenómeno migratorio y b el reclamo como práctica política fundamental en la vida cotidiana (urbana de los migrantes. Es objetivo ofrecer una reflexión sobre las complejas articulaciones entre política, clase y nacionalidad que se abren a partir de un proceso local de puja por el suelo urbano y por el derecho a la ciudad.

  6. Impact of burnout and psychosocial work characteristics on future long-term sickness absence. Prospective results of the Danish PUMA-study among human service workers

    DEFF Research Database (Denmark)

    Borritz, Marianne; Christensen, KB; Rugulies, R

    2010-01-01

    Objectives: The objective of this study was to examine if burnout and psychosocial factors predicted long-term sickness absence (>2 weeks) at work unit level. Methods: Data were collected prospectively at 82-work units in human services (PUMA cohort, PUMA: Danish acronym for Burnout, Motivation a...... work environment, and equally important, the organizations should be attentive to employees with symptoms of burnout...... and Job satisfaction) followed up during the proceeding 18 months regarding onset of long-term sickness absence. Questionnaire data regarding burnout and psychosocial factors were aggregated at work unit level. We used Poisson regression models with psychosocial factors and burnout as predictors of long...

  7. Repression of p53-target gene Bbc3/PUMA by MYSM1 is essential for the survival of hematopoietic multipotent progenitors and contributes to stem cell maintenance.

    Science.gov (United States)

    Belle, J I; Petrov, J C; Langlais, D; Robert, F; Cencic, R; Shen, S; Pelletier, J; Gros, P; Nijnik, A

    2016-05-01

    p53 is a central mediator of cellular stress responses, and its precise regulation is essential for the normal progression of hematopoiesis. MYSM1 is an epigenetic regulator essential for the maintenance of hematopoietic stem cell (HSC) function, hematopoietic progenitor survival, and lymphocyte development. We recently demonstrated that all developmental and hematopoietic phenotypes of Mysm1 deficiency are p53-mediated and rescued in the Mysm1(-/-)p53(-/-) mouse model. However, the mechanisms triggering p53 activation in Mysm1(-/-) HSPCs, and the pathways downstream of p53 driving different aspects of the Mysm1(-/-) phenotype remain unknown. Here we show the transcriptional activation of p53 stress responses in Mysm1(-/-) HSPCs. Mechanistically, we find that the MYSM1 protein associates with p53 and colocalizes to promoters of classical p53-target genes Bbc3/PUMA (p53 upregulated modulator of apoptosis) and Cdkn1a/p21. Furthermore, it antagonizes their p53-driven expression by modulating local histone modifications (H3K27ac and H3K4me3) and p53 recruitment. Using double-knockout mouse models, we establish that PUMA, but not p21, is an important mediator of p53-driven Mysm1(-/-) hematopoietic dysfunction. Specifically, Mysm1(-/-)Puma(-/-) mice show full rescue of multipotent progenitor (MPP) viability, partial rescue of HSC quiescence and function, but persistent lymphopenia. Through transcriptome analysis of Mysm1(-/-)Puma(-/-) MPPs, we demonstrate strong upregulation of other p53-induced mediators of apoptosis and cell-cycle arrest. The full viability of Mysm1(-/-)Puma(-/-) MPPs, despite strong upregulation of many other pro-apoptotic mediators, establishes PUMA as the essential non-redundant effector of p53-induced MPP apoptosis. Furthermore, we identify potential mediators of p53-dependent but PUMA-independent Mysm1(-/-)hematopoietic deficiency phenotypes. Overall, our study provides novel insight into the cell-type-specific roles of p53 and its downstream

  8. Eletrocardiografia em onças-pardas (Puma concolor anestesiadas com sevoflurano ou isoflurano

    Directory of Open Access Journals (Sweden)

    A.R. Oliveira

    Full Text Available RESUMO O objetivo deste trabalho foi descrever os achados eletrocardiográficos de 11 onças- pardas (Puma concolor. Os animais foram sedados com 0,15mg/kg de detomidina associado a 5mg/kg de cetamina e mantidos anestesiados com sevoflurano (GSEVO, n=6 ou isoflurano (GISO, n=5. A frequência cardíaca foi de 95 ± 13bpm. As alterações observadas nos animais no GSEVO foram: atrial standtill com condução ventricular, episódios isolados de contração ventricular prematura, bloqueio atrioventricular de primeiro grau, diminuição da amplitude do complexo QRS, onda S profunda e aumento da amplitude da onda T. No grupo GISO, observou-se bloqueio de ramo direito do feixe de His, bloqueio atrioventricular de primeiro grau e aumento da amplitude da onda T. Arritmias não puderam ser associadas ao uso dos anestésicos inalatórios devido à não sensibilização do miocárdio às catecolaminas. Achados como o BAV de primeiro grau pode ter ocorrido devido ao uso de agonistas α-2 adrenérgicos. Este estudo aumentou o conhecimento sobre as alterações eletrocardiográficas em onças-pardas anestesiadas, entretanto mais estudos são necessários para correlacionar estes achados ao uso de agentes anestésicos.

  9. Diet of margay, Leopardus wiedii, and jaguarundi, Puma yagouaroundi, (Carnivora: Felidae in Atlantic Rainforest, Brazil

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    Rita de Cassia Bianchi

    2011-02-01

    Full Text Available This study identifies the food habits of the margay, Leopardus wiedii (Schinz, 1821, and the jaguarundi, Puma yagouaroundi (É. Geoffroy Saint-Hilare, 1803, in the Vale do Rio Doce Natural Reserve and in the Sooretama Biological Reserve, Espírito Santo, Brazil. We determined the diet of both species by the analysis of scats. Fecal samples were collected from April 1995 to September 2000 and identified based on the presence of hairs that were ingested during self-grooming. Scats were oven-dried and washed on a sieve, and the screened material was identified using a reference collection. Of the 59 fecal samples examined, 30 were confirmed to be from the margay and nine of them from the jaguarundi. Mammals were the most consumed items in the diet of the margay, occurring in 77% of the fecal samples, followed by birds (53% and reptiles (20%. Among the mammals consumed, marsupials (Didelphimorphia were the most common item (66%. In the diet of the jaguarundi, birds were the most consumed items and occurred in 55% of the fecal samples; mammals and reptiles occurred in 41% and in 17% of the fecal samples, respectively. From this work we conclude that the margay and jaguarundi fed mainly upon small vertebrates in the Vale do Rio Doce Natural Reserve and in the Sooretama Biological Reserve. Although sample sizes are therefore insufficient for quantitative comparisons, margays prey more frequently upon arboricolous mammals than jaguarundis, which in turn prey more frequently upon birds and reptiles than margays. This seems to reflect a larger pattern throughout their geographic range

  10. El pensamiento integral en el desempeño escolar en estudiantes de Educación Básica Secundaria del Liceo Andino de la Santísima Trinidad de Finlandia Quindío

    OpenAIRE

    Lozano Loaiza, Luis Rodrigo

    2002-01-01

    Tesis (Maestría en Educación y Desarrollo Humano). Universidad de Manizales. Facultad de Ciencias Sociales y Humanas. CINDE, 2002 Este trabajo investigativo surge como una respuesta a la necesidad de conocer cómo la educación en básica secundaria, en particular en los estudiantes del Liceo Andino de la Santísima Trinidad, favorece el pensamiento integral y en él, la interdependencia y complementariedad de los tres estados del pensamiento tales como el lógico, el crítico y el cr...

  11. Inclusión-exclusión. Discusión y ejemplos del ámbito andino-patagónico

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    Conrado Santiago Bondel

    2014-12-01

    Full Text Available RESUMEN Este aporte se estructuró a partir del lema “Geografía: el desafío de construir territorios de inclusión”, lema convocante en las jornadas geográficas desarrolladas en Tandil en abril de 2014. Se propone un planteo de discusión a partir de, por un lado aceptar la complejidad intrínseca de la temática y su potencialidad para ser abordada desde innumerables enfoques y, por el otro, de valorar el peso relativo que sugieren las lógicas territoriales prevalentes en espacios-tiempos definidos. Es así que se toma el caso andino-patagónico actual como caso de análisis, destacando que las investigaciones que se vienen llevando a cabo en la región no han tenido esta temática por objetivo principal, pero aun así se sugieren situaciones ilustrativas y tal vez de futuras proyecciones propositivas. ABSTRACT The present contribution was organized after the motto that summons this meeting, “Geography: the challenge to construct territories of inclusion”. The proposal is to arrive to the discussion in order to accept the intrinsic complexity of the thematic and its potentially many ways to deal with the matter. Also to be able to appreciate the relative weight that territorial logics prevails as is defined in space-time suggestions. This is why the actual Andean-patagónico situation is taken as a matter of study, noting that this particular matter in present investigations is not been taken into account as a major goal. Even so illustrative situations are suggested as well as future proposals projections.

  12. Evaluación ambiental del proceso de urbanización de las cuencas del piedemonte andino de Santiago de Chile

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    Hugo Romero

    2005-12-01

    Full Text Available Las cuencas son complejos sistemas ambientales en que se desarrollan procesos geomorfológicos, hidrológicos, climáticos y ecológicos, que son impactados por la urbanización. En este trabajo se estudian seis cuencas del piedemonte andino de la ciudad de Santiago que se encuentran en diferentes fases del proceso de urbanización, variando entre Macul-San Ramón, en donde casi el 40% de su superficie ha sido incorporada a la ciudad, y Pirque, que mantiene aún sus características agrícolas y rurales. Empleando Sistemas de Información Geográfica, se analiza el impacto de la urbanización sobre indicadores ambientales, tales como productividad vegetal, biomasa, humedad en el suelo y temperaturas superficiales de los territorios ocupados por las cuencas. Las Áreas Totales de Impermeabilización, los Coeficientes de Escorrentía y las Áreas Sensitivas Ambientales, permiten estimar el estado de las cuencas y su contribución a la salud ambiental de la ciudad de SantiagoWatersheds are complex environmental systems, where geomorphologic, hydrologic, climatic and ecological processes take place, and which are severely impacted by urbanisation. In this paper, six Santiago Andean piedmont watersheds have been researched, according with the degree of urbanisation that they reach, which varies between Macul-San Ramón, where about 40% of their land covers has been already urbanised, until Pirque, where agricultural and rural features are still dominants. Using geographical information systems, the impact of urbanization on several ecological indicators, such as vegetative productivity, biomass, soil moisture and surface temperature are analysed. Total Imperviousness Areas, Runoff Coefficients and Environmentally Sensitive Areas allow the estimation of the state of the watersheds ant their contribution to the environmental health of Santiago city

  13. Géneros y formatos para la televisión digital. Análisis en los países andinos

    Directory of Open Access Journals (Sweden)

    Abel Suing

    2016-05-01

    Full Text Available El propósito de la investigación es analizar los formatos de televisión vigentes en la transición hacia la televisión digital terrestre en los países andinos e identificar alternativas para la televisión local. La investigación se justifica en razón del apagón analógico entre el 2018 y 2020. La metodología es cualitativa y cuantitativa; los instrumentos son análisis de contenidos y entrevistas semiestructuradas. Los resultados permiten concluir que los contenidos de producción nacional están vinculados con la actualidad. Los elementos que influyen en la creación de contenidos están relacionados con factores de identidad, recursos económicos, talento humano y narrativas. La hibridación de formatos que promuevan la cultura es una opción para las estaciones locales. The purpose of the research is to analyze the current television formats in the transition to digital terrestrial television in the Andean countries, and to identify alternatives for the local television. The research is justified in view of the analog blackout that will occur between 2018 and 2020. The methodology is both qualitative and quantitative; the instruments are content analyses and semi-structured interviews. The results allow concluding that the local production contents are linked to current events. The elements that influence the creation of contents are related to factors of identity, economic resources, human talent and narratives. The hybridization of formats promoting culture is an option for the local stations.

  14. La televisión social en los países andinos. Análisis de los medios sociales en informativos y magazines

    Directory of Open Access Journals (Sweden)

    Abel Suing

    2015-12-01

    Full Text Available Los países andinos transitan hacia la televisión digital al mismo tiempo en que se consolidan los medios sociales que otorgan a la audiencia la capacidad de interactuar en tiempo real, ello deriva en interrogantes como ¿existe integración de los medios sociales en la televisión?, ¿la audiencia participa en la definición de la televisión digital? y ¿estamos frente a la televisión social? La investigación pretende responder a las preguntas señaladas mediante el estudio de los programas de noticias y magazines de las estaciones de televisión líderes del área andina, en razón de que sus contenidos se emiten en tiempo real cuando se generaría el mayor interés de la audiencia. La metodología empleada es cuantitativa y cualitativa a partir del análisis de cuentas en Facebook y Twitter de las estaciones. Las hipótesis son que la televisión social gana aceptación en los países del área andina y que los canales de televisión no explotan los medios sociales. Se concluye que las estaciones privadas muestran más actividad que las públicas, que existe diferencia entre los informativos y el entretenimiento en seguidores y actividad en la red y que estamos en el inicio de la televisión social pero demanda más esfuerzos para integrar pantallas y medios sociales.

  15. Identificación de grupos funcionales de plantas en bordes de avance con potencial para la restauración de un Bosque Alto Andino

    Directory of Open Access Journals (Sweden)

    Bonilla María Argenis

    2006-06-01

    Full Text Available El estudio de grupos funcionales de plantas es una herramienta útil en la identificación de características de importancia en la restauración de una comunidad. El objetivo de este estudio es la definición de grupos funcionales de plantas en bordes de avance de un bosque alto andino y la evaluación de su importancia en el curso de la sucesión secundaria en pastizales dominados por Holcus lanatus. Con base en levantamientos de vegetación de
    10 x 10 m y en la revisión de atributos vitales de las especies registradas, se realizó una clasificación multivariante de las especies en grupos emergentes de plantas. Los atributos más importantes para la clasificación de los grupos emergentes fueron el método de dispersión y la ramificación del tronco en las especies leñosas. Se definieron cuatro grupos para las especies de estrato herbáceo y tres para las del estrato arbustivo-arbóreo, los
    cuales reúnen especies con estrategias de respuesta ante las condiciones que se presentan en los bordes estudiados.
    Dentro de los grupos definidos, las especies herbáceas dispersadas por diversos medios abióticos y las
    especies arbustivas dispersadas por viento y por aves parecen ser claves en la colonización del terreno dominado
    por H. lanatus y en la facilitación del establecimiento de especies de bosque secundario.

  16. The Effects of Gamma Irradiation on the Growth and Propagation of In-Vitro Chrysanthemum Shoot Explants (cv. Yellow Puma

    Directory of Open Access Journals (Sweden)

    I. Dwimahyani

    2010-08-01

    Full Text Available The study on the effect of gamma irradiation on in-vitro shoot growth of chrysanthemum cv. Yellow Puma has been carried out. The aim of the study was to observe genetic variability of shoot growth caused by gamma irradiation. Shoot explants with four leaves were irradiated by gamma with dose of 10, 15 and 20 Gy with 3 replications at each of dose. The irradiated shoot explants were then transferred into fresh MS solid medium and placed in a growth room. Observation was performed on number of leaves and branches on M1V0 generation, while plantlets height and number of branches were observed a M1V1 generation. Number of survival plantlets and multiplication rate on three subsequent subcultures were observed as well. Results showed that gamma rays with dose of 20 Gy inhibited growth of leaves as much as 50% compared to control (shoots without irradiation, and branches 73.7% in three weeks. Observation on multiplication rate at M1V1 generation showed that gamma irradiation with dose of 10 Gy promoted multiplication rate as much as 10% higher than control. It can be concluded that in vitro mutagenesis using gamma iradiation with dose of 10 to 15 Gy can be used for inducing genetic variability of chrysanthemum cv. Yellow Puma.

  17. p53 efficiently suppresses tumor development in the complete absence of its cell-cycle inhibitory and proapoptotic effectors p21, Puma, and Noxa.

    Science.gov (United States)

    Valente, Liz J; Gray, Daniel H D; Michalak, Ewa M; Pinon-Hofbauer, Josefina; Egle, Alex; Scott, Clare L; Janic, Ana; Strasser, Andreas

    2013-05-30

    Activation of apoptosis through transcriptional induction of Puma and Noxa has long been considered to constitute the critical (if not sole) process by which p53 suppresses tumor development, although G1/S boundary cell-cycle arrest via induction of the CDK inhibitor p21 has also been thought to contribute. Recent analyses of mice bearing mutations that impair p53-mediated induction of select target genes have indicated that activation of apoptosis and G1/S cell-cycle arrest may, in fact, be dispensable for p53-mediated tumor suppression. However, the expression of Puma, Noxa, and p21 was not abrogated in these mutants, only reduced; therefore, the possibility that the reduced levels of these critical effectors of p53-mediated apoptosis and G1/S-cell-cycle arrest sufficed to prevent tumorigenesis could not be excluded. To resolve this important issue, we have generated mice deficient for p21, Puma, and Noxa (p21-/-puma-/-noxa-/- mice). Cells from these mice were deficient in their ability to undergo p53-mediated apoptosis, G1/S cell-cycle arrest, and senescence. Nonetheless, these animals remained tumor free until at least 500 days, in contrast to p53-deficient mice, which had all succumbed to lymphoma or sarcoma by 250 days. Interestingly, DNA lesions induced by γ-irradiation persisted longer in p53-deficient cells compared to wild-type or p21-/-puma-/-noxa-/- cells, and the former failed to transcriptionally activate several p53 target genes implicated in DNA repair. These results demonstrate beyond a doubt that the induction of apoptosis, cell-cycle arrest, and possibly senescence is dispensable for p53-mediated suppression of spontaneous tumor development and indicate that coordination of genomic stability and possibly other processes, such as metabolic adaptation, may instead be critical.

  18. Densidad y preferencia de hábitat del perezoso de dos dedos Choloepus hoffmanni en un bosque andino (Bojacá, Cundinamarca, Colombia

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    Polanco Rocío

    2004-12-01

    Full Text Available Se estimó la densidad de perezosos de dos dedos Choloepus hoffmanni en un bosque andino fragmentado, a partir del conteo de restos fecales en parcelas fijas. Se caracterizó la vegetación del área y los árboles usados por los perezosos, con el fin de evaluar la preferencia de hábitat a dos escalas diferentes, así como la relación de las características del hábitat sobre la densidad de perezosos y la frecuencia de uso de los árboles. La densidad de perezosos en el área de estudio fue de 1,5 individuos/ha y varió entre tres fragmentos de bosque evaluados, diferentes en composición y estructura. Las densidades estimadas en las tres áreas de bosque resultaron
    en amplios intervalos de confianza, lo que es común a técnicas de conteo de heces. Aunque ninguna de las variables evaluadas de la vegetación explicó las diferencias de densidad entre bosques, el mayor desarrollo de la vegetación arbórea de una de las áreas de bosque dominado por Quercus humboldtii, y la evasión de la especie Miconia tonduzzi por los perezosos explicaría las diferencias en la densidad de perezosos, así como la evasión de una de las áreas de bosque. C. hoffmanni utiliza una gran variedad de especies arbóreas, pero con mayor frecuencia las especies Q. humboldtii y Brunellia tonduzii. Los árboles donde se realizaron avistamientos difieren de los árboles cercanos a restos fecales de Choloepus hoffmanni en cuanto a altura, DAP y porcentaje de la copa expuesta al sol. Ninguna de las variables evaluadas fue relevante para el uso
    de algunas especies de árboles con mayor frecuencia.

  19. DEVOCIONES DE INMIGRANTES: INDÍGENAS ANDINOS Y PLURIETNICIDAD URBANA EN LA CONFORMACIÓN DE COFRADÍAS COLONIALES (SANTIAGO DE CHILE, SIGLO XVII

    Directory of Open Access Journals (Sweden)

    Jaime Valenzuela Márquez

    2010-06-01

    Full Text Available En los orígenes de Chile colonial nos encontramos con numerosos indígenas andinos que sirvieron a los españoles, se asentaron y se integraron en los espacios laborales y sociales de este “nuevo mundo” periférico; formaron redes y parentescos locales, tuvieron descendencia, varios se transformaron en artesanos y algunos dictaron testamentos o dejaron huellas en documentos eclesiásticos y notariales que nos permiten observar su participación en las cofradías de la capital chilena. Estas corporaciones, por su parte, si bien remiten a una finalidad eminentemente religiosa, también actúan como un canal privilegiado para la inserción y movilidad de población diversa. Bajo esta perspectiva, nuestro artículo analiza las principales cofradías en las cuales participaron estos inmigrantes, vinculando prácticas religiosas, redes sociales y espacios laborales. Las cofradías estudiadas se revelan, entonces, como instancias representativas de la diversidad de orígenes geográficos y étnicos de sus integrantes, así como de la pluralidad de referentes identitarios que circulaban e interactuaban en un escenario urbano colonial como Santiago de Chile.In the origins of colonial Chile it is possible to find numerous Andean Indians who worked for Spaniards, settled and were integrated in labor and social spaces of this peripheral “new world”. They established local nets and relationships, and had descendants. Many became craftmen and some left wills or traces on ecclesiastic and notarial documents that allow us to observe their participation in brotherhoods created in Chile’s capital. These corporations had a religious aim, but worked as privileged channels for the insertion and mobility of diverse people. Under this premise, this article analyzes the main brotherhoods in which these immigrants participated, linking religious practices with their social nets and working spaces. The brotherhoods studied reveal themselves as instances that

  20. The multianalyser system of the three axes neutron spectrometer PUMA: Pilot experiments with the innovative multiplex technique

    Science.gov (United States)

    Sobolev, Oleg; Hoffmann, Ron; Gibhardt, Holger; Jünke, Norbert; Knorr, Andreas; Meyer, Volker; Eckold, Götz

    2015-02-01

    A new type of multiplex technique for three axes neutron spectrometers has been realized and successfully commissioned at the PUMA spectrometer at FRM II. Consisting of eleven analyser-detector channels which can be configured individually, this technique is especially suitable for kinetic experiments where a single excitation spectrum is recorded as a function of time without the need to move the spectrometer. On a time-scale of seconds an entire spectrum can be recorded thus allowing users to monitor changes during fast kinetic processes in single shot experiments without the need for stroboscopic techniques. Moreover, the multianalyser system provides an efficient and rapid tool for mapping excitations in (Q,ω)-space. The results of pilot experiments demonstrate the performance of this new technique and a user-friendly software is presented which assists users during their experiments.

  1. The essential role of p53-up-regulated modulator of apoptosis (Puma) and its regulation by FoxO3a transcription factor in β-amyloid-induced neuron death.

    Science.gov (United States)

    Akhter, Rumana; Sanphui, Priyankar; Biswas, Subhas Chandra

    2014-04-11

    Neurodegeneration underlies the pathology of Alzheimer disease (AD). The molecules responsible for such neurodegeneration in AD brain are mostly unknown. Recent findings indicate that the BH3-only proteins of the Bcl-2 family play an essential role in various cell death paradigms, including neurodegeneration. Here we report that Puma (p53-up-regulated modulator of apoptosis), an important member of the BH3-only protein family, is up-regulated in neurons upon toxic β-amyloid 1-42 (Aβ(1-42)) exposure both in vitro and in vivo. Down-regulation of Puma by specific siRNA provides significant protection against neuron death induced by Aβ(1-42). We further demonstrate that the activation of p53 and inhibition of PI3K/Akt pathways induce Puma. The transcription factor FoxO3a, which is activated when PI3K/Akt signaling is inhibited, directly binds with the Puma gene and induces its expression upon exposure of neurons to oligomeric Aβ(1-42). Moreover, Puma cooperates with another BH3-only protein, Bim, which is already implicated in AD. Our results thus suggest that Puma is activated by both p53 and PI3K/Akt/FoxO3a pathways and cooperates with Bim to induce neuron death in response to Aβ(1-42).

  2. Prevalence of human papillomavirus infection in rural villages of the Bolivian Amazon Prevalência da infecção pelo papilomavirus humano nas aldeias rurais do Amazonas Boliviano

    Directory of Open Access Journals (Sweden)

    Jorge Cervantes

    2003-06-01

    Full Text Available Cervical cancer constitutes a major health problem in developing countries like Bolivia. The roles of certain genotypes of human papillomaviruses (HPVs in the pathogenesis of cervical cancer is well established. The prevalence of HPV infection among sexually active women varies greatly. Information regarding HPV infection in Bolivia is very much scarce, specially in regions like the Amazonian lowland. We studied 135 healthy women living in four rural localities of the Bolivian Amazon. Presence of HPV in DNA extracted from cervical swabs was analyzed using a reverse line hybridization assay. The estimated overall HPV infection prevalence among the studied rural localities was 5.9% (ranging from 0-16.6%. These values were unexpectedly low considering Bolivia has a high incidence of cervical cancer. The fact that Amazonian people seem to be less exposed to HPV, makes it likely that some other risk factors including host lifestyle behaviors and genetic background may be involved in the development of cervical cancer in this population.O câncer cervical constitui problema de saúde da maior importância nos países em desenvolvimento tais como a Bolívia. O papel de certos genótipos de papilomavirus humano (HPVs na patologia do câncer cervical está bem estabelecido. A prevalência da infecção pelo HPV nas mulheres sexualmente ativas varia amplamente. Contudo, as informações existentes acerca da infecção pelo HPV na Bolivia são muito escassas, especialmente nas zonas rurais, nomeadamente na região amazônica do país. Foram estudadas 135 mulheres saudáveis, residentes em quatro localidades do Amazonas Boliviano. Foi detectada a presença de HPV no DNA extraído de esfregaços cervicais, utilizando o método de hibridização em linha reversa. A prevalência da infecção por HVP, encontrada nas localidades rurais, foi de 5,9% (variação entre 0-16,6%. Estes valores foram inesperadamente baixos, uma vez que a Bolívia é um país com alta

  3. Parámetros genético poblacionales en seis especies de Felidae neotropicales ( Leopardus tigrina, L. wiedii, L. pardalis, Herpailurus jagouroundi, Puma concolor y Pantera onca

    Directory of Open Access Journals (Sweden)

    Ruiz-García M.

    2001-06-01

    Full Text Available Se analizaron 196 muestras pertenecientes a 68 Leopardus pardalis (Ocelote; Colombia, Perú, a12 L. wiedi (Margay; Colombia y Bolivia, a 24 L. tigrinus (Tigrillo; Colombia, a 16 Herpailurus jagouroundi (yagouroundi; Colombia, Venezuela, Brasil a 50 Puma concolor (Puma; Colombia, Perú, Bolivia y a 24 Panthera onca (Jaguar; Colombia con 6 marcadores microsatélites diferentes (FCA08, FCA43, FCA45, FCA90, FCA96 y FCA126. Los resultados y conclusiones más obvias fueron las siguientes: (1 Para la mayoría de esas especies no se dio equilibrio Hardy- Weinberg cuando se analizaron individuos de localidades diferentes por exceso de homocigotos. Probablemente, el efecto Wahlund es responsable de ese hecho.

  4. Adaptaciones morfológicas de las hojas en una zona de bosque alto andino en la Reserva Natural Ranchería en Paipa, Boyacá, Colombia

    OpenAIRE

    2005-01-01

    La hoja es uno de los órganos más importantes de las plantas y tanto su morfología como su fisiología dependen de los factores ambientales de la zona donde se encuentren y de los organismos con los cuales tengan que competir para sobrevivir. En este trabajo se hizo una descripción de la morfología de las hojas de las plantas dicotiledóneas, colectadas en una zona de
    bosque alto andino en la “Reserva Municipal Natural  ranchería”, ubicada entre los 2.400- 3.400 m.s.n.m, en Paipa, Bo...

  5. Análisis genético molecular del Oso Andino ( Tremarctos ornatus en el norte de los andes (Venezuela, Colombia, Ecuador: Una visión global

    Directory of Open Access Journals (Sweden)

    Ruiz-García M.

    2001-06-01

    Full Text Available Se analizaron 82 muestras de oso andino (Tremarctos ornatus procedentes de 3 países latinoamericanos, Venezuela, Colombia y Ecuador, mediante 5 microsatélites hipervariables (G1A, G1D,
    G10B, G10C y G10X. Un exhaustivo análisis genético poblacional permitió determinar los siguientes aspectos fundamentales: (1 No existencia de equilibrio Hardy-Weinberg ni en el conjunto global de muestras ni en el conjunto particular de muestras para cada uno de esos países, al utilizar los tests exactos con cadenas de Markov y con el método de Fisher.

  6. Environmental quality for jaguar, puma and ocelot in Guaratuba Bay, State of Paraná, using the programs Capture and Presence

    Directory of Open Access Journals (Sweden)

    Matthias Ludwig Alfons Hammer

    2008-06-01

    Full Text Available Two month-long expeditions to Guaratuba Bay in the southern Atlantic Forest of Brazil were conducted in 2006. Parameters calculated from vestiges and camera-trap sampling were mammalian richness and proportion of area occupied (PAO by jaguar, puma, and opportunistically, by ocelot. Eight quadrats 4km2 in size were sampled, over an area of 130km2, where fourteen species of mammals were recorded (CI(Nˆ =14 to 14, CAPTURE. PAO was 25% for puma, and 100% for both jaguar and ocelot. It is argued that jaguar was expected to have capture probabilities similar to those of puma, as they both leave signs on open trails when present. The resulting PAO for jaguar is thus likely to be an artifact derived from low area substantiated by the reduced presence of important prey species in many of the sampling quadrats. The results do not diminish the importance of the study site for jaguar conservation; instead, these observations objectively identify the need to restore prey populations in the area.

  7. Lo Andino como marca planetaria

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    Fabio Aldemar Gómez Sierra

    2010-10-01

    Full Text Available La cadena montañosa más grande de la tierra atraviesa Suramérica; en ella, la vida parece haber encontrado la cuna ideal para expresar exuberancia, abundancia y diversidad. Aquí, las especies nativas y extranjeras parecen competir por mayor expresión y seducción. En los vegetales por ejemplo, lo cromático y aromático de los caducifolios traídos al altiplano Cundiboyacense, emulan con las características de los tomates, las granadillas o los mortiños. Asímismo, muchas familias de humanos y animales, continúan buscando este territorio para hospedarse y quedarse.

  8. Development and testing of an optimized method for DNA-based identification of jaguar (Panthera onca) and puma (Puma concolor) faecal samples for use in ecological and genetic studies.

    Science.gov (United States)

    Haag, Taiana; Santos, Anelisie S; De Angelo, Carlos; Srbek-Araujo, Ana Carolina; Sana, Dênis A; Morato, Ronaldo G; Salzano, Francisco M; Eizirik, Eduardo

    2009-07-01

    The elusive nature and endangered status of most carnivore species imply that efficient approaches for their non-invasive sampling are required to allow for genetic and ecological studies. Faecal samples are a major potential source of information, and reliable approaches are needed to foster their application in this field, particularly in areas where few studies have been conducted. A major obstacle to the reliable use of faecal samples is their uncertain species-level identification in the field, an issue that can be addressed with DNA-based assays. In this study we describe a sequence-based approach that efficiently distinguishes jaguar versus puma scats, and that presents several desirable properties: (1) considerably high amplification and sequencing rates; (2) multiple diagnostic sites reliably differentiating the two focal species; (3) high information content that allows for future application in other carnivores; (4) no evidence of amplification of prey DNA; and (5) no evidence of amplification of a nuclear mitochondrial DNA insertion known to occur in the jaguar. We demonstrate the reliability and usefulness of this approach by evaluating 55 field-collected samples from four locations in the highly fragmented Atlantic Forest biome of Brazil and Argentina, and document the presence of one or both of these endangered felids in each of these areas.

  9. p53's mitochondrial translocation and MOMP action is independent of Puma and Bax and severely disrupts mitochondrial membrane integrity

    Institute of Scientific and Technical Information of China (English)

    Sonja Wolff; Susan Erster; Gustavo Palacios; Ute M Moll

    2008-01-01

    p53's apoptotic program consists of transcription-dependent and transcription-independent pathways. In the latter, physical interactions between mitochondrial p53 and anti-and pro-apoptotic members of the Bcl2 family of mitochondrial permeability regulators are central. Using isogenic cell systems with defined deficiencies, we characterize in detail how mitochondrial p53 contributes to mitochondrial permeabilization, to what extent its action depends on other key Bcl2 family members and define its release activity. We show that mitochondrial p53 is highly efficient in inducing the release of soluble and insoluble apoptogenic factors by severely disrupting outer and inner mitochondrial membrane integrity. This action is associated with wild-type p53-induced oligomerization of Bax, Bak and VDAC and the formation of a stress-induced endogenous complex between p53 and cyclophilin D, normally located at the inner membrane. Tumor-derived p53 mutants are deficient in activating the Bax/Bak lipid pore. These actions are independent of Puma and Bax. Importantly, the latter distinguishes the mitochondrial from the cytosolic p53 death pathway.

  10. The R/V EL PUMA and JUSTO SIERRA impact on the development of oceanography in Mexico

    Science.gov (United States)

    Gracia, A.

    2007-05-01

    The acquisition of the two research vessels (R/V EL PUMA AND R/V JUSTO SIERRA) of the Universidad Nacional Autónoma de México represented a milestone for the development of oceanography and capacity building in Mexico. These boats were designed to conduct multi and interdisciplinary research in the Economic Exclusive Zone of Mexico in the main areas of oceanography (Physics, Geology, Chemistry and Biology). Its use, by different institutions, resulted in a substantial advancement of the knowledge of Marine ecosystems of Mexico. About 460 oceanographic campaigns, with more than 8700 participants, have been conducted since the boats arrived. These covered a wide array of topics of the marine ecosystem from the inner shelf to deep sea. Extensive research was done on current patterns, primary productivity and pollution monitoring of the Mexican Pacific, Sea of Cortez, Gulf of Mexico and Caribbean sea. Marine biodiversity studies were also carried which discovered more than 180 new species in Mexican seas. Ecological characterization and paleo-oceanographic research from continental shelf to deep sea also registered a substantial advance. The vessels are now renewed with hi-tech equipment for sea bottom, water column and navigation that increased their research capacity, representing again a new milestone in the history of oceanography in Mexico. This improved capacity is very promising and opens new and sound opportunities for carrying modern oceanographic in order to improve knowledge of the Mexican Economic Exclusive Zone.

  11. Activity and population characteristics of Andean Condors in southern Chile Actividad y características poblacionales de los Cóndores Andinos en el sur de Chile

    Directory of Open Access Journals (Sweden)

    RONALD J. SARNO

    2000-03-01

    Full Text Available Data were collected on general activity patterns and population characteristics of free-ranging Andean Condors Vultur gryphus in Torres del Paine National Park, Chile from July 1992 to June 1994 during 3,680 h of observation. Seasonal differences were evident in relative abundance and activity patterns. The mean number of condors sighted/ observation significantly higher in fall-winter than in spring-summer. There was a significant decrease from spring-summer to fall-winter in the percentage of observations during which we sighted solitary condors and a significant increase in the number of groups of condors. In addition, mean monthly maximum group size was significantly larger in fall-winter than spring-summer. The sex ratio (males:females of juveniles was significantly skewed in favor of females, and that of adults was significantly skewed in favor of males. The significantly different adult:juvenile ratio of condors visiting the park could have resulted from differences in distribution and habitat use and/or low breeding rates. Differential juvenile mortality and dispersal could also have produced skewed age and sex ratios, but more research is needed. Condor activity seemed related to wind speed. The greatest proportion of condors was observed flying in calm and low winds and less frequently in moderate to very strong winds. Temperature seemed to have an important effect on aerial activity because the greatest proportion of condors was sighted flying and soaring on warm days. Temperature and wind speed were weakly correlated.Se recolectaron datos sobre los patrones generales de actividad y caractersticas de la población del Cóndor Andino Vultur gryphus. El estudio se realizó en el Parque Nacional Torres del Paine, Chile, entre julio 1992 y junio de 1994, totalizando 3680 horas de observación. Durante el estudio fueron evidentes diferencias estacionales en los patrones de actividad y abundancia relativa de estas aves. El numero promedio

  12. Genetic Relationships of Common Bean (Phaseolus vulgaris L. Race Chile with Wild Andean and Mesoamerican Germplasm Relaciones Genéticas entre el Germoplasma de Poroto (Phaseolus vulgaris L. Raza Chile y Silvestres Andinos y Mesoamericanos

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    Viviana Becerra V

    2011-03-01

    Full Text Available The Chilean common bean (Phaseolus vulgaris L. belongs to the cultivated race Chile and its origin is presumably Andean. The objective of this study was to identify the origin of a group of Chilean accessions based on their genetic relationship with wild material from the Mesoamerican and Andean common bean gene pool. To achieve this objective, universal primers of chloroplast DNA (cpDNA and mitochondrial DNA (mtDNA were used to detect polymorphism using Polymerase Chain Reaction - Restriction Fragment Length Polymorphism (PCR-RFLP. Thirty-two genotypes were analyzed, including wild material from Mexico, Ecuador, Peru, Bolivia, and Argentina, as well as Chilean cultivated genotypes belonging to endemic Chilean accession types (Tórtola, Coscorrón, and Cuyano and naturalized commercial lines (Frutilla, Bayo, Manteca, and Blanco grande. Results showed a low level of polymorphism for cpDNA (23% and mtDNA (24% in wild and cultivated Chilean common bean accessions. Some universal primers and restriction enzyme combinations were more efficient than others in detecting polymorphism. The Chilean materials were closely related to wild accessions collected in Argentina, Bolivia, and Peru indicating their Andean origin. The wild accessions from Ecuador were located in a intermediate position between the Mesoamerican and Andean accessions.El poroto chileno (Phaseolus vulgaris L. pertenece a la raza Chile, cuyo origen es desconocido y presumiblemente andino. El objetivo del trabajo fue identificar el origen de un grupo de genotipos chilenos basado en sus relaciones genéticas con material silvestre perteneciente a los acervos genéticos mesoamericano y andino. Para lograr este objetivo se usaron partidores universales de ADNcp y ADNmt con la metodología de la Reacción en Cadena de la Polimerasa-Fragmentos de Restricción Polimórficos. (PCR-RFLP. Se analizó un total de 32 genotipos de P. vulgaris, los cuales incluyeron materiales silvestres de M

  13. A social-democracia do MAS boliviano

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    Guilherme Simões Reis

    2013-12-01

    Full Text Available O partido MAS, que governa a Bolívia e é liderado pelo presidente Evo Morales, geralmente é classificado como populista ou como revolucionário. Este artigo contesta ambos os diagnósticos, e sustenta que o MAS é um partido social-democrata. Tanto em sua gênese, como em seu comportamento na oposição, como em suas políticas no governo, o MAS apresenta todas as características necessárias para ser classificado como um representante da social-democracia. Para contestar os diagnósticos predominantes na literatura, o argumento desenvolve-se aplicando três distintas abordagens sobre a social-democracia. Uma comparação histórico-ideológica com os partidos tradicionalmente apontados como social-democratas mostra que o MAS assemelha-se a eles tanto em sua origem fortemente sindical como no tipo de mudança que introduziu na política do país. Uma análise institucional mostra que não procedem as acusações de que é antissistema e contrário à democracia, características associadas tanto aos "populistas", de acordo com "teóricos das duas esquerdas latino-americanas", como aos partidos adeptos da "revolução violenta". Por fim, uma análise das políticas adotadas pelo MAS no governo indica que estão alinhadas com aquelas consideradas como social-democráticas no contexto de integração dos mercados globais. Argumenta-se no texto que o MAS não é em geral classificado como social-democrata, em parte devido a uma visão equivocada sobre suas práticas, e em parte por uma tendência dos estudiosos a chamarem a atenção para o que lhe é específico, e não para o que ele tem em comum com outros partidos de esquerda, como é feito com quaisquer partidos ao catalogá-los em famílias.

  14. Los conflictos ambientales del gas boliviano

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    Gavaldá Palacín, Marc

    2005-01-01

    Full Text Available Los ajustes neoliberales introducidos en la legislación bolivana han facilitado la apropiación de los recursos hidrocarburíferos por parte de las transnacionales petroleras. Estas compañías invaden territorios indígenas y áreas protegidas. En su accionar generan graves conflictos ambientales con las comunidades locales. La costrucción de los gasoductos Bolivia-Brasil, Cuiabá y Gasyrg así como la oposición de la población a exportar gas a Estados Unidos nos reflejan cómo la población se organiza para resistir al expolio de las compañías petroleras. Las movilizaciones populares de ¿La Guerra del gas¿, en octubre 2003 no han terminado, porque el gas sigue en manos extranjeras.

  15. p53 Efficiently Suppresses Tumor Development in the Complete Absence of Its Cell-Cycle Inhibitory and Proapoptotic Effectors p21, Puma, and Noxa

    Directory of Open Access Journals (Sweden)

    Liz J. Valente

    2013-05-01

    Full Text Available Activation of apoptosis through transcriptional induction of Puma and Noxa has long been considered to constitute the critical (if not sole process by which p53 suppresses tumor development, although G1/S boundary cell-cycle arrest via induction of the CDK inhibitor p21 has also been thought to contribute. Recent analyses of mice bearing mutations that impair p53-mediated induction of select target genes have indicated that activation of apoptosis and G1/S cell-cycle arrest may, in fact, be dispensable for p53-mediated tumor suppression. However, the expression of Puma, Noxa, and p21 was not abrogated in these mutants, only reduced; therefore, the possibility that the reduced levels of these critical effectors of p53-mediated apoptosis and G1/S-cell-cycle arrest sufficed to prevent tumorigenesis could not be excluded. To resolve this important issue, we have generated mice deficient for p21, Puma, and Noxa (p21−/−puma−/−noxa−/− mice. Cells from these mice were deficient in their ability to undergo p53-mediated apoptosis, G1/S cell-cycle arrest, and senescence. Nonetheless, these animals remained tumor free until at least 500 days, in contrast to p53-deficient mice, which had all succumbed to lymphoma or sarcoma by 250 days. Interestingly, DNA lesions induced by γ-irradiation persisted longer in p53-deficient cells compared to wild-type or p21−/−puma−/−noxa−/− cells, and the former failed to transcriptionally activate several p53 target genes implicated in DNA repair. These results demonstrate beyond a doubt that the induction of apoptosis, cell-cycle arrest, and possibly senescence is dispensable for p53-mediated suppression of spontaneous tumor development and indicate that coordination of genomic stability and possibly other processes, such as metabolic adaptation, may instead be critical.

  16. Plasma membrane lipid alterations associated with cold acclimation of winter rye seedlings (Secale cereale L. cv Puma)

    Energy Technology Data Exchange (ETDEWEB)

    Lynch, D.V.; Steponkus, P.L. (Cornell Univ., Ithaca, NY (USA))

    1987-01-01

    Highly enriched plasma membrane fractions were isolated from leaves of nonacclimated (NA) and acclimated (ACC) rye (Secale cereale L. cv Puma) seedlings. Collectively, free sterols, steryl glucosides, and acylated steryl glucosides constituted > 50 mole % of the total lipid in both NA and ACC plasma membrane fractions. Glucocerebrosides containing hydroxy fatty acids constituted the major glycolipid class of the plasma membrane, accounting for 16 mole % of the total lipid. Phospholipids, primarily phosphatidylcholine and phosphatidylethanolamine with lesser amounts of phosphatidylglycerol, phosphatidic acid, phosphatidylserine, and phosphatidylinositol, comprised only 32 mole% of the total lipid in NA samples. Following cold acclimation, free sterols increased from 33 to 44 mole %, while steryl glucosides and acylated steryl glucosides decreased from 15 to 6 mole % and 4 to 1 mole %, respectively. Sterol analyses of these lipid classes demonstrated that free {beta}-sitosterol increased from 21 to 32 mole % (accounting for the increase in free sterols as a class) at the expense of sterol derivatives containing {beta}-sitosterol. Glucocerebrosides decreased from 16 to 7 mole % of the total lipid following cold acclimation. In addition, the relative proportions of associated hydroxy fatty acids, including 22:0 (h), 24:0 (h), 22:1 (h), and 24:1 (h) were altered. The phospholipid content of the plasma membrane fraction increased to 42 mole % of the total lipid following cold acclimation. Although the relative proportions of the individual phospholipids did not change appreciably after cold acclimation, there were substantial differences in the molecular species. Di-unsaturated molecular species of phosphatidylcholine and phosphatidylethanolamine increased following acclimation. These results demonstrate that cold acclimation results in substantial changes in the lipid composition of the plasma membrane.

  17. Pride diaries: sex, brain size and sociality in the African lion (Panthera leo) and cougar (Puma concolor).

    Science.gov (United States)

    Arsznov, Bradley M; Sakai, Sharleen T

    2012-01-01

    The purpose of this study was to examine if differences in social life histories correspond to intraspecific variation in total or regional brain volumes in the African lion (Panthera leo) and cougar (Puma concolor). African lions live in gregarious prides usually consisting of related adult females, their dependent offspring, and a coalition of immigrant males. Upon reaching maturity, male lions enter a nomadic and often, solitary phase in their lives, whereas females are mainly philopatric and highly social throughout their lives. In contrast, the social life history does not differ between male and female cougars; both are solitary. Three-dimensional virtual endocasts were created using computed tomography from the skulls of 14 adult African lions (8 male, 6 female) and 14 cougars (7 male, 7 female). Endocranial volume and basal skull length were highly correlated in African lions (r = 0.59, p < 0.05) and in cougars (r = 0.67, p < 0.01). Analyses of total endocranial volume relative to skull length revealed no sex differences in either African lions or cougars. However, relative anterior cerebrum volume comprised primarily of frontal cortex and surface area was significantly greater in female African lions than males, while relative posterior cerebrum volume and surface area was greater in males than females. These differences were specific to the neocortex and were not found in the solitary cougar, suggesting that social life history is linked to sex-specific neocortical patterns in these species. We further hypothesize that increased frontal cortical volume in female lions is related to the need for greater inhibitory control in the presence of a dominant male aggressor.

  18. Cylicospirura species (Nematoda: Spirocercidae) and stomach nodules in cougars (Puma concolor) and bobcats (Lynx rufus) in Oregon.

    Science.gov (United States)

    Ferguson, Jayde A; Woodberry, Karen; Gillin, Colin M; Jackson, DeWaine H; Sanders, Justin L; Madigan, Whitney; Bildfell, Robert J; Kent, Michael L

    2011-01-01

    The stomachs and proximal duodena of 160 cougars (Puma concolor) and 17 bobcats (Lynx rufus), obtained throughout Oregon during 7 yr, were examined for Cylicospirura spp. and associated lesions. Prevalence in cougars was 73%, with a range in intensity of 1-562 worms. The mean diameter of nodules was 1.2 cm (SD=0.5), and many extended through the submucosa to the muscularis. About 83% of cougars had nodules; most nodules contained worms, but 14% of the smaller nodules (<0.2 cm) contained porcupine (Erethizon dorsatum) quills. A mean of 12.4 worms/nodule (SD=34.1) was observed, with a maximum of 340 worms/nodule. Prevalence in bobcats was 53%, with an intensity of 1-25 worms. About 65% of bobcats had nodules, which were slightly smaller than those in cougars but appeared to involve similar layers of gastrointestinal tissue. One to 25 Cylicospirura sp. were found in all but two small nodules in bobcats. Cougars killed for livestock damage or safety concerns had a significantly higher median worm intensity than did those that died of other causes. Also, the median worm intensity of older cougars was higher than that of younger lions. There were more males than females killed for livestock damage or safety concerns. The cylicospirurid from cougars was Cylicospirura subaequalis, and that of bobcats was Cylicospirura felineus. These two similar species were separated morphologically by differences in tooth and sex organ morphology. They were also differentiated by DNA sequence analysis of the mitochondrial cytochrome c oxidase subunit 1 gene (cox1). Worm sequences from cougars differed from those from bobcats by 11%, whereas essentially no difference was found among worms from the same host. Phylogenetic analysis showed that within the order Spirurida, both cylicospirurids were most closely related to Spirocerca lupi, based on this gene sequence.

  19. ADUBAÇÃO ORGÂNICA EM CONDIÇÕES DE IRRIGAÇÃO SUPLEMENTAR E SEU EFEITO NA PRODUTIVIDADE DA QUINUA (Chenopodium quinoa Willd) NO PLANALTO DA BOLÍVIA

    OpenAIRE

    Roberto Miranda Casas

    2012-01-01

    A quinua (Chenopodium quinoa Willd) é um grão andino, com elevado valor proteico (14%), sendo um dos principais cultivos que geram rendas para os agricultores do Planalto Boliviano. A cultura desenvolve-se em condições de baixa temperatura, precipitação pluvial e fertilidade e, embora tenha um bom desenvolvimento em condições com limitações ambientais e de solo, os rendimentos usualmente observados no Planalto Boliviano são baixos. A presente pesquisa teve como objetivo avaliar a produtividad...

  20. Effects of PUMA/ASPP1 Gene on Proliferation of Gastric Cancer SGC-7901 Cells%PUMA/ASPP1双抑癌基因对胃癌 SGC-7901细胞增殖的影响

    Institute of Scientific and Technical Information of China (English)

    李军; 龚辉; 朱水山

    2015-01-01

    目的:探讨 p53正向细胞凋亡调控因子(p53 up-regulated modulator of apoptosis protein,PUMA)/p53促凋亡蛋白(apoptosis stimulating protein of p53,ASPP)1融合基因及单个基因对胃癌 SGC-7901细胞的杀伤作用。方法通过脂质体(lipofectamine)2000TM 将 PUMA/ASPP1融合基因及 PUMA、ASPP1单个基因分别转染胃癌SGC-7901细胞系[实验设5组,分别为胃癌细胞无转染空白对照(SGC-7901)组,空载质粒转染细胞对照(SGC-7901-pcDNA3.1)组以及重组 ASPP1质粒转染细胞实验(SGC-7901-ASPP1)组、重组 PUMA 质粒转染细胞实验(SGC-7901-PUMA)组、重组 PUMA/ASPP1质粒转染细胞实验(SGC-7901-PUMA/ASPP1)组],再次经 G418筛选,获得稳定表达融合基因 SGC-7901-PUMA/ASPP1细胞株。通过 MTT 法及使用流式细胞仪测定各组胃癌SGC-7901系细胞的存活数(光吸收度即 A 值)及细胞周期。结果SGC-7901组与 SGC-7901-pcDNA3.1组 A 值比较差异无统计学意义(P >0.05);SGC-7901-ASPP1组、SGC-7901-PUMA 组和 SGC-7901-PUMA/ASPP1组 A值明显低于 SGC-7901组与 SGC-7901-pcDNA3.1组(均 P <0.01);SGC-7901-ASPP1组、SGC-7901-PUMA 组和SGC-7901-PUMA/ASPP13组中,SGC-7901-PUMA/ASPP1细胞组 A 值最低(P <0.01)。与 SGC-7901组、SGC-7901-pcDNA3.1组比较,SGC-7901-ASPP1、SGC-7901-PUMA、SGC-7901-PUMA/ASPP1组的 G1期明显增多,S 期明显减少,尤以 SGC-7901-PUMA/ASPP1组 S 期减少为甚(均 P <0.01);SGC-7901-pcDNA3.1组与 SGC-7901组比较 G1期、S 期差异无统计学意义(P >0.05)。结论双抑癌基因可有效抑制胃癌 SGC-7901系细胞增殖,双抑癌基因较单个抑癌基因具有更强的抗肿瘤作用。%ABSTRACT:Objective To investigate the lethal effects of p53 up-regulated modulator of apopto-sis(PUMA),apoptosis stimulating protein of p53(ASPP1 )and PUMA-ASPP1 fusion gene on hu-man gastric cancer SGC-7901 cells

  1. Roscovitine-induced apoptosis in neutrophils and neutrophil progenitors is regulated by the Bcl-2-family members Bim, Puma, Noxa and Mcl-1.

    Directory of Open Access Journals (Sweden)

    Sanjivan Gautam

    Full Text Available Neutrophil granulocyte (neutrophil apoptosis plays a key role in determining inflammation in infectious and non-infectious settings. Recent work has shown that inhibitors of cyclin-dependent kinases (cdk such as roscovitine can potently induce neutrophil apoptosis and reduce inflammation. Using a conditional Hoxb8-expression system we tested the participation of Bcl-2-family proteins to roscovitine-induced apoptosis in mouse neutrophils and in neutrophil progenitor cells. Bcl-2 strongly protected against roscovitine-induced apoptosis in neutrophils. The isolated loss of either Bim or noxa provided significant, partial protection while protection through combined loss of Bim and noxa or Bim and Puma was only slightly greater than this individual loss. The only substantial change in protein levels observed was the loss of Mcl-1, which was not transcriptional and was inhibited by proteasome blockade. In progenitor cells there was no protection by the loss of Bim alone but substantial protection by the loss of both Bim and Puma; surprisingly, strongest protection was seen by the isolated loss of noxa. The pattern of protein expression and Mcl-1-regulation in progenitor cells was very similar to the one observed in differentiated neutrophils. In addition, roscovitine strongly inhibited proliferation in progenitor cells, associated with an accumulation of cells in G2/M-phase.

  2. Contesting the Equivalency of Continuous Sedation until Death and Physician-assisted Suicide/Euthanasia: A Commentary on LiPuma.

    Science.gov (United States)

    Raho, Joseph A; Miccinesi, Guido

    2015-10-01

    Patients who are imminently dying sometimes experience symptoms refractory to traditional palliative interventions, and in rare cases, continuous sedation is offered. Samuel H. LiPuma, in a recent article in this Journal, argues that continuous sedation until death is equivalent to physician-assisted suicide/euthanasia based on a higher brain neocortical definition of death. We contest his position that continuous sedation involves killing and offer four objections to the equivalency thesis. First, sedation practices are proportional in a way that physician-assisted suicide/euthanasia is not. Second, continuous sedation may not entirely abolish consciousness. Third, LiPuma's particular version of higher brain neocortical death relies on an implausibly weak construal of irreversibility--a position that is especially problematic in the case of continuous sedation. Finally, we explain why continuous sedation until death is not functionally equivalent to neocortical death and, hence, physician-assisted suicide/euthanasia. Concluding remarks review the differences between these two end-of-life practices.

  3. Sevoflurane Post-conditioning Protects Primary Rat Cortical Neurons Against Oxygen-Glucose Deprivation/Resuscitation: Roles of Extracellular Signal-Regulated Kinase 1/2 and Bid, Bim, Puma.

    Science.gov (United States)

    Zhang, Limin; Zhao, Xiaochun; Jiang, Xiaojing

    2015-08-01

    Temporal post-conditioning to induce neuroprotection against brain ischemia-reperfusion injury insult is considered to be an effective intervention, but the exact mechanisms of sevoflurane post-conditioning are poorly understood. Extracellular signal-related kinases 1/2 (Erk1/2) play a pivotal role in the cell growth and proliferation. The essential axis of activator Bid, Bim, Puma (BH3s) and BAX, BAK in activating the mitochondrial death program might offer common ground for cell death signal. We hypothesized that, sevoflurane post-conditioning might inhibit the expression of Bid, Bim and Puma and is activated by phosphor-Erk1/2 to reduce neuronal death. To test this hypothesis, we exposed primary cultured cortical neurons to oxygen-glucose deprivation for 1 h and resuscitation for 24 h (OGD/R). The assays of MTT, propidium iodide uptake, JC-1 fluorescence and western blot demonstrated that OGD/R exposure reduced cell viability, increased cell death, decreased mitochondrial membrane potential and the expressions of Bid, Bim, and Puma. Inhibition of Erk1/2 phosphorylation could partially attenuate 2 % of sevoflurane post-conditioning mediated increase in neuronal viability and mitochondrial membrane potential, and also a decrease in cell death and expression of Bid, Bim and Puma after OGD/R treatment. The results demonstrated that, the protection of sevoflurane post-conditioning markedly reducing death of cortical neurons exposed to OGD/R could be correlated with down-regulation of Bid, Bim and Puma expression mediated by phosphorylation/activation of Erk1/2.

  4. Registros de ocurrencia del oso andino (Tremarctos ornatus Cuvier, 1825 en sus límites de distribución nororiental y austral Occurrence of the Andean bear (Tremarctos ornatus Cuvier, 1825 in its north eastern and southern limit of distribution.

    Directory of Open Access Journals (Sweden)

    J. Fernando Del Moral Sachetti

    2011-06-01

    Full Text Available El conocimiento de la distribución del oso andino (Tremarctos ornatus, es fundamental para evaluar el estado de conservación de la especie y el desarrollo de medidas de conservación tales como la declaración de nuevas áreas protegidas o la implementación de estrategias de manejo. El presente estudio tuvo por objetivo ejecutar relevamientos de campo entre los años 2007 y 2008, en los límites del rango distributivo andino nororiental y austral de la especie, mediante el método de rastreo en crestas de serranías afín de conseguir datos de primera mano sobre la presencia/ausencia del oso andino en estas áreas. Seguidamente, se presentan 101 registros actuales en total obtenidos del taxón, entre la región centro-occidental de Venezuela y el extremo noroccidental de Argentina. Finalmente, se discuten las implicancias biogeográficas de los datos reportados.The knowledge of the Andean bear's (Tremarctos ornatus distribution is fundamental for evaluating the status of the species and the development of conservation measures such as the declaration of new conservation areas or the implementation of the management measures. This study aimed perform some field assessments between the years 2007 and 2008, in the Andean north eastern and southern limits of the species' range, using the method of tracking mountain ridges to obtain first hand data on the presence / absence of the Andean bear in these areas. We have obtained 101 current records of the species, including the central-western of Venezuela and the northwest tip of Argentina. Finally, we discuss the biogeographic implications of the reported data.

  5. PUMA gene inhibits the growth of PC-3 Pancreatic carcinoma cells%PUMA基因转染对胰腺癌细胞生长的影响

    Institute of Scientific and Technical Information of China (English)

    张克君; 李德春; 朱新国; 朱东明; 赵志泓

    2006-01-01

    目的 探讨PUMA基因转染抑制胰腺肿瘤生长的体内外效果.方法 利用脂质体转染法将表达PUMA的质粒转染导入胰腺癌细胞株PC-3中,G418筛选出阳性克隆,Western和RT-PCR法检测PUMA转染后PC-3 PUMA的表达,流式细胞仪检测转染后细胞凋亡率;分别将转染PUMA的PC-3细胞(实验组)和未转染的PC-3细胞移植到裸鼠体内,比较裸鼠移植肿瘤的大小和重量以及PUMA表达.结果 PUMA表达质粒转染的PC-3细胞(PC-3/PUMA)稳定表达PUMA,其细胞凋亡率为(5.50 ± 0.90)%,明显高于未转染组的(1.073 ± 0.248)%和空载体转染组的(1.08 ± 0.35)%(P <0.05);裸鼠接种4周后PC-3/PUMA细胞成瘤率为70%,PC-3细胞和空载体PC-3细胞成瘤率为100%(P > 0.05),PC-3/PUMA细胞形成的肿瘤体积比PC-3细胞和空载体PC-3细胞明显减小(P <0.05),并且形成的肿瘤组织中PUMA高表达.结论 胰腺癌细胞中缺失PUMA基因表达,PUMA转染胰腺癌细胞后表达PUMA,并能促进细胞凋亡.

  6. Cadenas productivas promisorias para la seguridad alimentaria e inclusión económica en familias pobres rurales : caso del proyecto Desarrollo sostenible de granos andinos y papas nativas en comunidades rurales altoandinas de la región Ayacucho implementado por CARE Perú 2009-2011

    OpenAIRE

    Nishikawa Menacho, Julio

    2016-01-01

    En la perspectiva de aportar en el desarrollo de políticas de inclusión social y económica, basada en evidencias, se ha desarrollado el presente trabajo de investigación cualitativa tomando como base la experiencia desarrollada por CARE PERU a través del proyecto “Desarrollo Sostenible de Granos Andinos y Papas Nativas en Comunidades Rurales Altoandinas” en el Departamento de Ayacucho. El objetivo central de la investigación fue identificar los principales factores de éxito que han contri...

  7. Indígenas andinos en Chile Colonial: Inmigración, inserción espacial, integración económica y movilidad social (Santiago, Siglos XVI-XVII

    Directory of Open Access Journals (Sweden)

    Valenzuela Márquez, Jaime

    2010-12-01

    Full Text Available Beginning with the 16th century conquering expeditions, many Andean natives who served the Spaniards arrived in Chile, where they settled in and integrated in the work and social areas of this outlying «New World»; they formed local networks and relationships, had offspring; a number of them became artisans and some drafted wills or left their mark in ecclesiastical or notarized documents that provide us with an idea of their mobility and local integration. This work offers a background and reveals the principle hubs of a process resembling a medullar structure, in order to understand how the early Chilean colonial society was made up. At the same time, it provides insights into the phenomena of movement, uprooting and settlement of the indigenous population in the southern Peruvian viceroyalty.

    Desde las expediciones de conquista del siglo XVI llegaron a Chile numerosos indígenas andinos que sirvieron a los españoles, se asentaron y se integraron en los espacios laborales y sociales de este «nuevo mundo» periférico; formaron redes y parentescos locales, tuvieron descendencia, varios se transformaron en artesanos y algunos dictaron testamentos o dejaron huellas en documentos eclesiásticos y notariales que nos permiten observar su movilidad e inserción local. Este trabajo aporta antecedentes y desvela los principales ejes de un proceso que aparece como medular para entender la formación de la sociedad colonial chilena temprana y, a la vez, para dar cuenta de fenómenos de circulación, desarraigo y asentamiento de población indígena en el virreinato peruano meridional.

  8. Both p53-PUMA/NOXA-Bax-mitochondrion and p53-p21cip1 pathways are involved in the CDglyTK-mediated tumor cell suppression

    Energy Technology Data Exchange (ETDEWEB)

    Yu, Zhendong, E-mail: zdyu@hotmail.com [Department of Clinical laboratory, Peking University Shenzhen Hospital, Guangdong (China); Wang, Hao [Department of pathology, The Chinese University of Hong Kong, Hong Kong (China); Zhang, Libin; Tang, Aifa; Zhai, Qinna; Wen, Jianxiang; Yao, Li [Department of Clinical laboratory, Peking University Shenzhen Hospital, Guangdong (China); Li, Pengfei, E-mail: lipengfei@cuhk.edu.hk [Department of pathology, The Chinese University of Hong Kong, Hong Kong (China)

    2009-09-04

    CDglyTK fusion suicide gene has been well characterized to effectively kill tumor cells. However, the exact mechanism and downstream target genes are not fully understood. In our study, we found that CDglyTK/prodrug treatment works more efficiently in p53 wild-type (HONE1) cells than in p53 mutant (CNE1) cells. We then used adenovirus-mediated gene delivery system to either knockdown or overexpress p53 and its target genes in these cells. Consistent results showed that both p53-PUMA/NOXA/Bcl2-Bax and p53-p21 pathways contribute to the CDglyTK induced tumor cell suppression. Our work for the first time addressed the role of p53 related genes in the CDglyTK/prodrug system.

  9. Exacerbations and health care resource utilization in patients with airflow limitation diseases attending a primary care setting: the PUMA study

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    Montes de Oca M

    2016-12-01

    . Asthma patients with exacerbations had twice the number of visits versus those without an exacerbation. The number of visits was higher (2.8 times in asthma–COPD overlap, asthma (1.9 times, or COPD (1.4 times patients versus those without these respiratory diseases; the number of visits due to exacerbation was also higher (4.9 times in asthma–COPD overlap, asthma (3.5 times, and COPD (3.8 times patients. Conclusion: COPD, asthma, and asthma–COPD overlap increase the prevalence of medical visits and, therefore, health care resource utilization. Attempts to reduce health care resource use in these patients require interventions aimed at preventing exacerbations. Keywords: COPD, asthma, asthma–COPD overlap, exacerbation, health care resource utilization, primary care, PUMA

  10. Chemical immobilization of captive Cougars Puma concolor (Linnaeus, 1771 (Carnivora: Felidae using a combination of tiletamine-zolazepam, ketamine and xylazine

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    Jesús Lescano

    2014-12-01

    Full Text Available Handling of large felids is highly risky, hence immobilization is required for the safety of personnel.  Data on the effects of anesthetic drugs used for immobilizing Cougars Puma concolor are scarce. This study describes the anesthetic and cardiorespiratory effects of a combination of tiletamine-zolazepam (2mg/kg, ketamine (1.6mg/kg and xylazine (0.4mg/kg in pumas. Five captive adult and clinically healthy Cougars were included in this study.  Animals were immobilized by remote injection using blow pipe and darts.  The durations of induction, immobilization and recovery periods and their qualities were recorded.  Heart rate, respiratory rate, body temperature and blood pressure were recorded at five minute intervals for 25 minutes.  Then all animals received 0.125mg/kg of yohimbine, intramuscularly administered.  Central tendency and dispersal statistics were calculated for each parameter.  The duration of the induction period was 10.4±6.4 minutes and the duration of the recovery period was 83.3±35.1 minutes. Induction, immobilization and recovery periods were smooth and adequate anesthetic depth was achieved.  The mean heart rate was 122±10 beats/minute, mean respiratory rate was 10±1 breaths/minute, mean body temperature was 39.1±0.2 0C and mean blood pressure was 139±12 mmHg.  No statistically significant difference (p>0.05 was observed in vital parameters over the duration of the assessment.  The tested anesthetic combination effectively immobilized the cougars included in this study and provided safety for the personnel involved.  Though vital signs were not significantly affected, a degree of hypoventilation was observed and respiratory support is recommended when using this anesthetic combination in cougars. 

  11. Sevoflurane post-conditioning protects primary rat cortical neurons against oxygen-glucose deprivation/resuscitation via down-regulation in mitochondrial apoptosis axis of Bid, Bim, Puma-Bax and Bak mediated by Erk1/2.

    Science.gov (United States)

    Zhang, Li-Min; Zhao, Xiao-Chun; Sun, Wen-Bo; Li, Rui; Jiang, Xiao-Jing

    2015-10-15

    Temporal post-conditioning helps provide neuroprotection against brain injury secondary to ischemia-reperfusion and is considered an effective intervention, but the exact mechanism of sevoflurane post-conditioning is unclear. The essential axis involves activator Bid, Bim, Puma (BH3s), Bax, and Bak; activates the mitochondrial death program; and might be involved in a cell death signal. Extracellular signal-related kinases 1/2 (Erk1/2) play a pivotal role in cell growth and proliferation. We hypothesized that sevoflurane post-conditioning might inhibit Bid, Bim, Puma, Bax, and Bak expression and is activated by phosphor-Erk1/2 to decrease neuronal death. To test this hypothesis, we exposed primary cortical neuron cultures to oxygen-glucose deprivation for 1h, along with resuscitation for 24h (OGD/R). MTT assays, propidium iodide uptake (PI), JC-1 fluorescence, and Western blot indicated the following: decreased cell viability (PPuma, Bax, and Bak expression with OGD/R exposure. Inhibition of Erk1/2 phosphorylation could attenuate sevoflurane post-conditioning that mediated an increase in neuronal viability and mitochondrial membrane potential, as well as a decrease in cell death and Bid, Bim, Puma, Bax, and Bak expression after OGD/R treatment. The results demonstrated that sevoflurane post-conditioning caused a marked decrease in cortical neuronal death secondary to OGD/R exposure through the downregulation of the mitochondrial apoptosis axis involving Bid, Bim, Puma, Bax, and Bak that was mediated by the phosphorylation/activation of Erk1/2.

  12. Estratigrafía y paleoambientes de la Formación Los Llanos (La Rioja: una secuencia condensada Miocena en el antepaís fragmentado andino central

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    M. Ezpeleta

    2006-06-01

    Full Text Available En la región central de las Sierras Pampeanas, un conjunto de afloramientos saltuarios conocidos como Formación Los Llanos, se disponen irregularmente a través de las sierras Brava, Los Llanos y Ulapes-Las Minas, formando parte de la cubierta cenozoica. Esta sucesión sedimentaria está dominantemente compuesta por niveles de calcretes y depósitos fluvio-eólicos, y su espesor raramente supera los ~40 m. Desde su definición, existen escasos trabajos de detalle sobre su estratigrafía desde una óptica moderna. El mapeo y análisis detallado de facies permitieron dividir a la unidad en miembros inferior y superior, y caracterizar sus paleoambientes sedimentarios. Para el conjunto se definieron ocho facies: 1 conglomerados clastos soportados, 2 brechas y microbrechas, 3 areniscas con megaestratificación cruzada, 4 conglomerados arenosos calcáreos fuertemente cementados, 5 areniscas y arcilitas con estructura columnar, 6 areniscas conglomerádicas con cemento silícico, 7 limo-arcilitas pardo-rojizas, y 8 brechas fangosas. Estas facies fueron agrupadas en las asociaciones de facies (I fluvio-eólica y (II aluviales con paleosuelos, que parcialmente coinciden con los miembros reconocidos. El conjunto permite deducir un paleoambiente de abanicos aluviales medios a distales periódicamente afectados por formación de paleosuelos. Estos últimos reflejan sectores distales con escasa influencia de relieve indicativos de episodios de condensación. Debido a los escasos registros paleontológicos con procedencia documentada, y hasta tanto se tengan edades geocronológicas precisas, nuestras interpretaciones paleoclimáticas permitirían realizar una correlación con los eventos climáticos del Mioceno Medio- Plioceno ampliamente difundidos en el antepaís andino, que habrían afectado a los depósitos sinorogénicos desarrollados en una zona de domamiento periférico.

  13. Ecological niche models and patterns of richness and endemism of the southern Andean genus Eurymetopum (Coleoptera, Cleridae Modelos de nicho ecológico y patrones de riqueza y endemismo del género andino austral Eurymetopum (Coleoptera, Cleridae

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    Tania Escalante

    2009-01-01

    Full Text Available Eurymetopum is an Andean clerid genus with 22 species. We modeled the ecological niches of 19 species with Maxent and used them as potential distributional maps to identify patterns of richness and endemicity. All modeled species maps were overlapped in a single map in order to determine richness. We performed an optimality analysis with NDM/VNDM in a grid of 1º latitude-longitude in order to identify endemism. We found a highly rich area, located between 32º and 41º south latitude, where the richest pixels have 16 species. One area of endemism was identified, located in the Maule and Valdivian Forest biogeographic provinces, which extends also to the Santiago province of the Central Chilean subregion, and contains four endemic species (E. parallelum, E. prasinum, E. proteus, and E. viride, as well as 16 non-endemic species. The sympatry of these phylogenetically unrelated species might indicate ancient vicariance processes, followed by episodes of dispersal. Based on our results, we suggest a close relationship between these provinces, with the Maule representing a complex area.Eurymetopum es un género de cléridos andinos con 22 especies. Modelamos los nichos ecológicos de 19 especies con Maxent y los utilizamos como mapas de distribución potencial para identificar patrones de riqueza y endemismo. Todos los mapas de las especies se superpusieron en un mapa único para determinar la riqueza. Realizamos un análisis de optimalidad con NDM/VNDM en una cuadrícula de 1º de latitud-longitud para identificar el endemismo. Hallamos un área de mayor riqueza, localizada entre los 32º y 41º de latitud sur, donde los pixeles más ricos poseen 16 especies. Se identificó un área de endemismo en las provincias biogeográficas del Maule y el Bosque Valdiviano, la cual se extiende también a la provincia de Santiago de la subregión Chilena Central, y que contiene cuatro especies endémicas (E. parallelum, E. prasinum, E. proteus y E. viride, as

  14. Reflexiones etnográficas sobre la «ciudadanía transnacional». Prácticas políticas de andinos en el sur de Europa

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    Suárez Navaz, Liliana

    2010-08-01

    Full Text Available This article explores from an ethnographic perspective the political practices of migrants in a transnational public sphere, based in a study case of Andean Ecuadorians in Spain. It emphasizes the connections between the impact of immigrants settling in Madrid and their efforts to maintain a socioeconomic and political presence in their community of origin, looking at the way new practices of transnational citizenship are established in current neoliberal age. It proposes the category of transnational social fields in the bourdieuan sense to scrutinize how territory, identity and residence are redefined to build up a new cartography of inclusion and exclusion from citizenship rights and duties across national frontiers. It offers some empirical findings to illustrate the way new structures of sociocultural stratification are built both in the origin and destiny countries through these new transnational political practices. These insights are contextualized within some key theoretical debates around the new proposals of transnational citizenship.

    Este trabajo explora desde una perspectiva etnográfica las prácticas políticas de migrantes en una esfera pública transnacional, en base a un estudio de caso de ecuatorianos andinos en España. Enfatiza las interconexiones que se producen entre el impacto del asentamiento de los inmigrantes en Madrid y sus esfuerzos por mantener una presencia socioeconómica y política activa en su comunidad de origen, analizando el modo en el que las nuevas prácticas de ciudadanía transnacional se configuran en la actual era neoliberal. Se propone la categoría de campo migratorio transnacional en el sentido bourdieuano para profundizar cómo el territorio, la identidad y la residencia se redefinen en una nueva cartografía de inclusión y exclusión de los derechos y deberes de ciudadanía a través de las fronteras. Se ofrecen algunos resultados empíricos que ilustran las nuevas estructuras de

  15. Estructura comunitaria de diatomeas presentes en los sedimentos superficiales de ocho lagos andinos de Chile central Diatoms community structure in superficial sediments of eight Andean lakes of central Chile

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    INGRID E ALVIAL

    2008-03-01

    Full Text Available En este trabajo se analizó la estructura comunitaria de diatomeas depositadas en los sedimentos superficiales de ocho lagos andinos chilenos ubicados entre los 32°49' y 38°48' S. Se encontró un total de 99 taxa de diatomeas, distribuidos en 48 géneros. Del total de taxa, 74 fueron identificados a nivel de especie siendo todos ellos cosmopolitas a excepción de Eunotia andinofrequens, Gomphonema punae, Pinnularia araucanensis y Pinnularia acidicola, entre otras, que están descritos solo para el Hemisferio Sur. Por otro lado, las muestras analizadas presentaron distinta composición florística de diatomeas. De esta manera, en los ensambles diatomológicos depositados en los sedimentos de las lagunas de altura Ocho, Huifa, Ensueño y Negra ubicadas sobre los 2.860 m del nivel del mar, abundaron especies bentónicas, típicas de aguas oligotróficas y acidas como Achnanthidium exiguum, Achnanthidium minutissimum, Encyonema minutum, Pinnularia acidicola y Planothidium lanceolatum. En los ensambles diatomológicos de los lagos Galletué, Icalma y Laja, ubicados bajo los 1.360 m del nivel del mar, abundaron diatomeas planctónicas, características de aguas alcalinas y mesotróficas como Asterionella formosa, Aulacoseira distans, Aulacoseira granulata, Cyclotella stelligera y Rhopalodia gibbaIn this research the taxonomic structure of diatoms in sediments of high mountain lakes was studied. These lakes are located in Chile between 32°49' and 38°48' S in the Andean Cordillera. A total of 99 diatom taxa distributed in 48 genera were identified and all this taxa are cosmopolitan excepting a Eunotia andinofrequens, Gomphonema punae, Pinnularia araucanensis and Pinnularia acidicola, which are know only for the Southern Hemisphere. The assemblages of diatoms were different in the studied lakes. So the high mountain lakes Ocho, Huifa, Ensueño and Negra, dominated benthic diatoms which are typical of oligotrophic and acid waters as Achnanthidium

  16. Recombinant adenovirus containing PUMA gene regulated by human telomerase reverse transcriptase promoter transfected into ovarian cancer COC1 cells%hTERT启动子调控的PUMA腺病毒转染卵巢癌COC1细胞的研究

    Institute of Scientific and Technical Information of China (English)

    李雨聪; 王冬

    2014-01-01

    Objective To over-express the P53 upregulated modulator of apoptosis(PUMA) in ovarian cancer COC1 cells for playing the role to restrain the cell growth .Methods Adenovirus vector (Ad)-human telomerase reverse transcriptase promoter (hTERT)-PUMA was transfected into ovarian cancer COC1 cells .Then the growth of COC1 cells was studied by CCK-8 and the apoptosis kit and the level of PUMA was analyzed .Results After transfection of Ad-hTERT-PUMA ,PUMA was over-expressed in COC1 cells ,which restrained the cells growth and promoted the cell apoptosis .Conclusion Ad-hTERT-PUMA is successfully transfected into ovarian cancer COC1 cells and PUMA is over-expressed to play the role for restraining the cells growth .%目的:探讨卵巢癌COC1细胞中大量表达p53正向凋亡调控因子(PUMA)在抑制COC1细胞中的作用。方法采用人端粒酶逆转录酶(hTERT)启动子调控的PUMA基因腺病毒载体(Ad-hTERT-PUMA)转染卵巢癌COC1细胞,通过细胞活性检测试剂盒和凋亡试剂盒研究COC1细胞的生长情况,同时分析表达PUMA的水平。结果转染Ad-hTERT-PUMA后,PU-MA大量表达,抑制COC1细胞生长,促进细胞凋亡。结论Ad-hTERT-PUMA成功转染卵巢癌COC1细胞并大量表达,起到了抑制卵巢癌细胞增长的作用。

  17. "A carne mais barata do mercado é a carne negra: uma reflexão sobre o "design" das camisas da Puma na Copa do Mundo de Futebol/2010 "The cheapest flesh in the market is the black one": body, soccer and advertising Puma uniforms in the 2010 soccer world cup

    Directory of Open Access Journals (Sweden)

    Victor Andrade de Melo

    2011-06-01

    Full Text Available A Puma, uma empresa de material esportivo, desde meados da primeira década do século XXI tem constantemente utilizado jogadores negros em suas campanhas publicitárias, parte de uma estratégia de busca de aproximação com o continente africano. À beira da falência no início dos anos 90, a empresa conseguiu se reposicionar no mercado unindo o esporte à moda, investindo em produtos de "design" arrojado. Uma de suas iniciativas foi buscar inspiração no "caráter exótico" das nações africanas para produzir peças que possam ser utilizadas em situações cotidianas, não só para a prática de esportes. Como o corpo do negro africano tem sido representado em suas estratégias publicitárias? Esse estudo teve por objetivo analisar um ponto específico da publicidade da Puma por ocasião da Copa do Mundo de Futebol de 2010, realizada na África do Sul: o "design" das camisas fornecidas a três seleções africanas - Camarões, Costa do Marfim e Gana. Para alcance do objetivo, entabulamos uma comparação com os modelos das camisas fornecidas pela empresa a outros selecionados (Suíça, Uruguai e Itália, prospectando o quanto suas ações publicitárias reforçam ou não certos estereótipos comumente observados quando se utilizam negros em campanhas de publicidade.Puma, a company of sport gears, has been inserting black soccer players in its advertising campaigns as a strategy to get closer to the African continent. Near to bankruptcy at the 90' beginning, the company has succeeded in bringing to the market products with more daring design, linking fashion and sports. One of its latest initiatives was to seek inspiration in the exotic profile of African people to produce pieces that can be used in all occasions not only for sports. How African's bodies have been exposed in its adverting campaigns? The objective of this study was to analyze an specific issue related to some strategies adopted in Puma advertising campaigns launched during

  18. Anti-tumour activity of a novel coumarin-chalcone hybrid is mediated through intrinsic apoptotic pathway by inducing PUMA and altering Bax/Bcl-2 ratio.

    Science.gov (United States)

    Singh, Neetu; Sarkar, Jayanta; Sashidhara, Koneni V; Ali, Shakir; Sinha, Sudhir

    2014-06-01

    Coumarins and chalcones are secondary plant metabolites which have shown an array of pharmacological properties including anti-tumour activity. We have previously reported on the synthesis and anti-proliferative activity of a series of novel coumarin-chalcone hybrids. Now we report on the in vivo efficacy as well as mechanism of action of the most potent molecule of the series, S009-131. Oral administration of this molecule resulted in regression of tumours induced by HeLa cell xenografts in nod SCID mice. The molecule inhibited proliferation of cervical cancer cells (HeLa and C33A) by inducing apoptosis and arresting cell cycle at G2/M phase. Apoptosis was induced through induction of caspase-dependent intrinsic pathway and alterations in the cellular levels of Bcl-2 family proteins. The mitochondrial transmembrane potential got highly depleted in S009-131 treated cells due to an increase in Bax/Bcl-2 ratio and intracellular ROS. The molecule induced release of cytochrome c into the cytosol and activation of initiator caspase-9 and executioner caspases-3/7. Tumour suppressor protein p53 and its transcriptional target PUMA were up regulated, suggesting their role in mediating the cell death. These results suggest that S009-131 is a potent candidate for the chemotherapy of cervical carcinoma.

  19. Jaguarundi (Puma yagouaroundi) (Geoffroy, 1803) (Carnivora, Felidae) food habits in a mosaic of Atlantic Rainforest and eucalypt plantations of southeastern Brazil.

    Science.gov (United States)

    Tófoli, C F; Rohe, F; Setz, E Z F

    2009-08-01

    Food habits of jaguarundi (Puma yagouaroundi) (Geoffroy, 1803) (Carnivora, Felidae) were studied between November 2000 and November 2001, in a 24.9 km(2) area of secondary Atlantic Rainforest and eucalypt plantation, in the Serra de Paranapiacaba, São Paulo State, Brazil. Analyses of 26 fecal and regurgitate samples, obtained over a stretch of 570.1 km, showed the consumption of 19 prey items and 74 prey occurrences. Small mammals were the most frequent food item (42.5%), followed by birds (21%), reptiles (14%) and medium-sized mammals (3%). The percent occurrence (PO) suggests that the diet consisted mainly of small rodents (30%) and birds (21%). We recorded for the first time the predation of Viperidae snakes by P. yagouaroundi. Although having a large list of items and range of dietary niche breadths (Bsta = 0.76), our data show that jaguarundi prey mainly on small vertebrates (mammals, birds or reptiles), and even in tall tropical forests or eucalypt plantations, it preys mostly on animals that come to, or live on, the ground.

  20. Evaluation of cardiopulmonary parameters and recovery from anesthesia in cougars (Puma concolor anesthetized with detomidine/ketamine and isoflurane or sevoflurane

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    Verônica B. Albuquerque

    2016-01-01

    Full Text Available Abstract: The aim of this study was to assess the cardiopulmonary effects, the onset time after the administration of a detomidine/ketamine combination, and the recovery from anesthesia of cougars (Puma concolor anesthetized with detomidine/ketamine and isoflurane or sevoflurane for abdominal ultrasound imaging. Fourteen animals were randomly allocated into two experimental groups: GISO (n=7 and GSEVO (n=7. Chemical restraint was performed using 0.15mg/kg detomidine combined with 5mg/kg ketamine intramuscularly; anesthesia induction was achieved using 2mg/kg propofol intravenously and maintenance with isoflurane (GISO or sevoflurane (GSEVO. The following parameters were assessed: heart rate, respiratory rate, systolic and diastolic arterial blood pressure, mean arterial blood pressure, oxyhemoglobin saturation, rectal temperature, central venous pressure, and end-tidal carbon dioxide. The time to sternal recumbency (TSR and time to standing position (TSP were also determined. There was not statistically significant difference for the cardiopulmonary variables or TSP whereas TSR was significantly shorter in GSEVO. The time to onset of anesthesia was 11.1±1.2 minutes and 11.3±1.8 minutes for GISO and GSEVO, respectively. The anesthesia of cougars with detomidine/ketamine and isoflurane or sevoflurane was conducted with safety, cardiopulmonary stability, and increased time to sternal recumbency in the GISO group.

  1. Survivin启动子调控的PUMA基因表达载体对乳腺癌MCF-7细胞凋亡的影响%Construction of a PUMA gene-targeted expression vector regulated by Survivin promoter and research of its effect on cell apoptosis in MCF-7 cells

    Institute of Scientific and Technical Information of China (English)

    陈绍坤; 刘晓华; 黄丽; 余红; 税青林

    2013-01-01

    目的:探讨构建带有肿瘤特异性Survivin启动子的PUMA基因表达载体pUC57-Survivin-PUMA对乳腺癌MCF-7细胞的肿瘤特异性促凋亡作用.方法:化学合成PUMA基因片段克隆至pUC57质粒,构建重组质粒pUC57-PUMA;克隆Survivin启动子序列,取代pUC57-PUMA质粒原有的CMV启动子,构建重组质粒pUC57-Survivin-PUMA,测序鉴定.实验设置pUC57-PUMA组、pUC57-Survivin-PUMA组、阴性对照组(pUC57group)和空白对照组(blank group).将以上各组分别转染人乳腺癌MCF-7细胞及人正常乳腺Hs 578Bst细胞.转染后48h,western blot技术检测各组细胞中PUMA蛋白表达情况,流式细胞仪检测细胞的凋亡率.结果:重组质粒经测序鉴定证实符合设计要求,构建成功;MCF-7细胞中pUC57-PUMA组和pUC57-Survivin-PUMA组的PUMA蛋白表达均较对照组显著增高(P<0.05),而人正常乳腺Hs 578Bst细胞中只有pUC57-PUMA组的PUMA蛋白表达较其他3组显著增高(P<0.05).MCF-7细胞中pUC57-PUMA组和pUC57-Survivin-PUMA组的细胞凋亡率分别为29.02%和29.31%,均较对照组显著增高(P<0.05),而人正常乳腺Hs 578Bst细胞中仅pUC57-PUMA组的细胞凋亡率较其他3组显著增高(P<0.05).结论:Survivin启动子调控的PUMA表达载体能显著增高乳腺癌MCF-7细胞中PUMA的表达,进而促进乳腺癌MCF-7细胞凋亡,而对正常乳腺Hs 578Bst细胞的凋亡不产生影响.%Objective:To construct PUMA gene-targeted expression vector driven by tumor specific Survivin promoter (pUC57-Survivin-PUMA) and investigate its cell apoptosis effects in MCF-7 breast cancer cells.Methods:The PUMA gene sequence by chemical synthesis was cloned in the plasmid pUC57 to construct the recombinant plasmid pUC57-PUMA,the CMV promoter in which then were replaced respectively by the Survivin promoter,and the plasmid pUC57-Survivin-PUMA was constructed finally.Four experimental groups were set up,they were pUC57-PUMA group,pUC57-Survivin-PUMA group,pUC57 control group

  2. Distribución espacio-temporal de larvas de Chironomidae (Diptera en un arroyo andino (Uspallata, Mendoza, Argentina Spatial and temporal distribution of larvae of Chironomidae (Diptera in an Andean stream (Uspallata, Mendoza, Argentina

    Directory of Open Access Journals (Sweden)

    Erica E. Scheibler

    2008-12-01

    Full Text Available Se realizó un estudio sobre la composición larval de la familia Chironomidae en un arroyo andino en la localidad de Uspallata (Mendoza, Argentina. Se realizaron muestreos estacionales durante un ciclo anual completo (2001/2002 en tres sitios, siguiendo un gradiente altitudinal. Se midieron variables físico-químicas en campo y laboratorio. La relación entre presencia y abundancia de quironómidos y parámetros ambientales se exploró mediante un análisis de Correspondencia Canónica. Se identificaron siete géneros pertenecientes a cuatro subfamilias: Chironominae, Orthocladiinae, Podonominae y Tanypodinae. Orthocladiinae fue la subfamilia que tuvo mayor riqueza genérica (Cricotopus van der Wulp, Onconeura Andersen & Saether y Parametriocnemus Goetghebuer, densidad y frecuencia de aparición a nivel estacional y espacial. Podonominae (Podonomus Philippi y Podonomopsis Brundin se halló únicamente en SA mientras que Chironominae (Polypedilum Kieffer estuvo presente en UD. Tanypodinae (Pentaneura Philippi se encontró en los tres sitios de muestreo aunque con una densidad menor. Se detectó incremento de la conductividad y temperatura del agua, y disminución del tamaño del sustrato desde SA a UD, lo que coincide con la desaparición de Podonomus y Podonomopsis y la aparición de Polypedilum hacia UD. De este estudio se concluye que el sistema presenta baja riqueza genérica comparada con otros sistemas montañosos de Argentina.A study was conducted on the larval composition of the family Chironomidae in an Andean stream in Uspallata locality (Mendoza, Argentina. Seasonal samplings were performed over a full annual cycle (2001/2002 at three sites, along an altitudinal gradient. Physical and chemical variables were measured in both the field and the laboratory. Relationship between presence and abundance of chironomids and environmental parameters was explored using a Canonical Correspondence analysis. Seven genera were identified

  3. Pintuco se internacionaliza en el mercado andino.

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    Carlos Enrique Ramírez R.

    2004-01-01

    Full Text Available La historia de la internacionalización de Pintuco está presentada en este trabajo. Es importante resaltar la valiosa información sobre el mercado mundial de pinturas lo cual despierta el interés del lector al mostrar el tamaño del mercado y la importancia económica del mismo. De igual forma, la introducción es muy expedita al señalar cuál es la tendencia de dicho mercado, pues con esto se puede hacer una idea de hacia dónde deberá ir Pintuco y cuáles serán sus principales retos a superar. Luego de dar un vistazo general de lo que son las pinturas y su impacto económico, el artículo continúa con la historia del Grupo Inversiones Mundial (GIM, ente económico que ha triunfado gracias a la tenacidad de sus directivos desde 1921. La estrategia utilizada por la empresa colombiana es explicada a través de los ejemplos de cómo empezó y se desarrolló en Venezuela y Ecuador, principalmente. Y es en esto, en lo que precisamente radica lo más importante del trabajo pues sirve como motivación y como ejemplo a seguir. Las empresas colombianas como Pintuco han entendido que una de las formas de amoldarse a las nuevas tendencias del mundo empresarial es la de competir con calidad, pero no sólo en su propio mercado, sino también en el mercado de origen de su competencia. Hoy en día las fronteras son cada vez más invisibles y eso, más que una amenaza, debería ser un atractivo.

  4. Desarrollo andino: sustentable y con identidad

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    Bernardo Guerrero Jiménez

    2013-07-01

    Full Text Available El concepto del desarrollo ocupa buena parte de la agenda de los Estados y los gobiernos en América Latina. Sin embargo, esta idea esconde algunos supuestos que en este artículo discutimos. El desarrollo y su vinculación a la identidad cultural, al territorio, contraviene la idea hegemónica que anima a este concepto nacido en Occidente. Analizamos el itinerario en América Latina y lo ubicamos en el Norte Grande de Chile, enfatizando las ideas centrales desde los años sesenta adelante del siglo XX. Finalizamos argumentando a favor del llamado posdesarrollo.

  5. The pan-Bcl-2 blocker obatoclax promotes the expression of Puma, Noxa, and Bim mRNA and induces apoptosis in neoplastic mast cells.

    Science.gov (United States)

    Peter, Barbara; Cerny-Reiterer, Sabine; Hadzijusufovic, Emir; Schuch, Karina; Stefanzl, Gabriele; Eisenwort, Gregor; Gleixner, Karoline V; Hoermann, Gregor; Mayerhofer, Matthias; Kundi, Michael; Baumgartner, Sigrid; Sperr, Wolfgang R; Pickl, Winfried F; Willmann, Michael; Valent, Peter

    2014-01-01

    Advanced SM is an incurable neoplasm with short survival time. So far, no effective therapy is available for these patients. We and others have shown recently that neoplastic MC in ASM and MCL express antiapoptotic Mcl-1, Bcl-2, and Bcl-xL. In this study, we examined the effects of the pan-Bcl-2 family blocker obatoclax (GX015-070) on primary neoplastic MC, the human MC leukemia cell line HMC-1, and the canine mastocytoma cell line C2. Obatoclax was found to inhibit proliferation in primary human neoplastic MC (IC₅₀: 0.057 μM), in HMC-1.2 cells expressing KIT D816V (IC₅₀: 0.72 μM), and in HMC-1.1 cells lacking KIT D816V (IC₅₀: 0.09 μM), as well as in C2 cells (IC₅₀: 0.74 μM). The growth-inhibitory effects of obatoclax in HMC-1 cells were accompanied by an increase in expression of Puma, Noxa, and Bim mRNA, as well as by apoptosis, as evidenced by microscopy, TUNEL assay, and caspase cleavage. Viral-mediated overexpression of Mcl-1, Bcl-xL, or Bcl-2 in HMC-1 cells was found to introduce partial resistance against apoptosis-inducing effects of obatoclax. We were also able to show that obatoclax synergizes with several other antineoplastic drugs, including dasatinib, midostaurin, and bortezomib, in producing apoptosis and/or growth arrest in neoplastic MC. Together, obatoclax exerts major growth-inhibitory effects on neoplastic MC and potentiates the antineoplastic activity of other targeted drugs. Whether these drug effects can be translated to application in patients with advanced SM remains to be determined.

  6. COPD Underdiagnosis and Misdiagnosis in a High-Risk Primary Care Population in Four Latin American Countries. A Key to Enhance Disease Diagnosis: The PUMA Study

    Science.gov (United States)

    Casas Herrera, Alejandro; Montes de Oca, Maria; López Varela, Maria Victorina; Aguirre, Carlos; Schiavi, Eduardo; Jardim, José R.

    2016-01-01

    Background Acknowledgement of COPD underdiagnosis and misdiagnosis in primary care can contribute to improved disease diagnosis. PUMA is an international primary care study in Argentina, Colombia, Venezuela and Uruguay. Objectives To assess COPD underdiagnosis and misdiagnosis in primary care and identify factors associated with COPD underdiagnosis in this setting. Methods COPD was defined as post-bronchodilator (post-BD) forced expiratory volume in 1 second/forced vital capacity (FEV1/FVC) <0.70 and the lower limit of normal (LLN). Prior diagnosis was self-reported physician diagnosis of emphysema, chronic bronchitis, or COPD. Those patients with spirometric COPD were considered to have correct prior diagnosis, while those without spirometric criteria had misdiagnosis. Individuals with spirometric criteria without previous diagnosis were considered as underdiagnosed. Results 1,743 patients were interviewed, 1,540 completed spirometry, 309 (post-BD FEV1/FVC <0.70) and 226 (LLN) had COPD. Underdiagnosis using post-BD FEV1/FVC <0.70 was 77% and 73% by LLN. Overall, 102 patients had a prior COPD diagnosis, 71/102 patients (69.6%) had a prior correct diagnosis and 31/102 (30.4%) had a misdiagnosis defined by post-BD FEV1/FVC ≥0.70. Underdiagnosis was associated with higher body mass index (≥30 kg/m2), milder airway obstruction (GOLD I–II), black skin color, absence of dyspnea, wheezing, no history of exacerbations or hospitalizations in the past-year. Those not visiting a doctor in the last year or only visiting a GP had more risk of underdiagnosis. COPD underdiagnosis (65.8%) and misdiagnosis (26.4%) were less prevalent in those with previous spirometry. Conclusions COPD underdiagnosis is a major problem in primary care. Availability of spirometry should be a priority in this setting. PMID:27073880

  7. Huéspedes definitivos de Spirometra mansonoides (Cestoda, Diphyllobothriidae en el Perú

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    Manuel Tantaleán

    2013-05-01

    Full Text Available Se realizó un estudio parasitológico en el zoológico Parque de Las Leyendas, Lima, Perú en el año de 1993. Se recolectaron 49 muestras de heces de carnívoros pertenecientes a cinco familias: Canidae, Ursidae, Procyonidae, Mustelidae y Felidae, éstas fueron procesadas usando métodos rutinarios para la búsqueda de huevos de helmintos. En las heces de tres especies de la familia Felidae, Puma concolor (puma andino y puma de la selva, Panthera onca (otorongo y Leopardus pardalis (tigrillo se identificaron huevos de Spirometra mansonoides.

  8. Concurrent acetylation of FoxO1/3a and p53 due to sirtuins inhibition elicit Bim/PUMA mediated mitochondrial dysfunction and apoptosis in berberine-treated HepG2 cells.

    Science.gov (United States)

    Shukla, Shatrunajay; Sharma, Ankita; Pandey, Vivek Kumar; Raisuddin, Sheikh; Kakkar, Poonam

    2016-01-15

    Post-translational modifications i.e. phosphorylation and acetylation are pivotal requirements for proper functioning of eukaryotic proteins. The current study aimed to decode the impact of acetylation/deacetylation of non-histone targets i.e. FoxO1/3a and p53 of sirtuins (NAD(+) dependent enzymes with lysine deacetylase activity) in berberine treated human hepatoma cells. Berberine (100 μM) inhibited sirtuins significantly (Pberberine potentiated sirtuins inhibition and increased the expression of FoxO1/3a and phosphorylation of p53 tumor suppressor protein. As sirtuins deacetylate non-histone targets including FoxO1/3a and p53, berberine increased the acetylation load of FoxO1/3a and p53 proteins. Acetylated FoxO and p53 proteins transcriptionally activate BH3-only proteins Bim and PUMA (3.89 and 3.87 fold respectively, Pberberine and nicotinamide was curtailed up to 28.3% (Pberberine (25 μM), reversed sirtuins expression comparable to control and significantly restored the cell viability (Pberberine mediated sirtuins inhibition resulting into FoxO1/3a and p53 acetylation followed by BH3-only protein Bim/PUMA activation may in part be responsible for mitochondria-mediated apoptosis.

  9. Impacto del pastoreo sobre picoplancton autotrófico en dos lagos andinos (Patagonia, Argentina con diferentes relaciones luz:nutrientes Grazing impact on autotrophic picoplankton in two south Andean lakes (Patagonia, Argentina with different light:nutrient ratios

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    ESTEBAN G. BALSEIRO

    2004-03-01

    Full Text Available Los lagos andino-patagónicos son ambientes con una alta relación luz:nutrientes. En estos lagos se ha descrito una red trófica particular constituida por grandes ciliados mixotróficos que comparten y compiten por recursos alimentarios con nanoflagelados y cladóceros. A través de experimentos de pastoreo se compararon las tasas de limpieza sobre picoplancton autotrófico de nanoflagelados; el ciliado Ophrydium naumanni y cladóceros de los lagos Moreno Oeste y Rivadavia. Estos lagos presentan diferencias significativas en cuanto a su relación luz:nutrientes y tienen también una composición de crustáceos del zooplancton diferente. En el lago Moreno Oeste, el metalimnion está incluido dentro de la zona eufótica, determinando un estrato iluminado en el que se desarrolla un máximo de clorofila en profundidad. En el lago Rivadavia, en cambio, los estratos iluminados están restringidos al epilimnion y no se observó ningún máximo de clorofila en profundidad. En el lago Moreno se observó que a 30 m de profundidad se producía un incremento en la proporción de la bacterivoría ejercida por el ciliado O. naumanni y el cladócero Ceriodaphnia dubia debido fundamentalmente a la distribución vertical de ambas especies. Por el contrario, las tasas de limpieza del conjunto de nanoflagelados, dominado por la especie mixótrofa Chrysochromulina parva, no presentaron cambios a lo largo de la columna de agua y fueron un orden de magnitud mayores que aquellas de O. naumanni y C. dubia. En el lago Rivadavia, las tasas de pastoreo de los nanoflagelados fueron menores y comparables con las obtenidas para O. naumanni y Daphnia cf. commutata. Las diferencias observadas en las tasas de limpieza de nanoflagelados probablemente reflejan un incremento de la fagotrofía donde la energía lumínica es alta en relación con el fósforo. De esta manera, en el lago Moreno Oeste, donde la luz no es limitante, el aumento observado en la bacterivoría de los

  10. Sensitivity of winter phytoplankton communities from Andean lakes to artificial ultraviolet-B radiation Sensibilidad de comunidades fitoplanctónicas invernales de lagos andinos a la radiación ultravioleta-B artificial

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    E. WALTER HELBLING

    2001-06-01

    Andinos con el objetivo de determinar la sensibilidad de comunidades fitoplanctónicas invernales a la radiación ultravioleta-B (UV-B, 280-320 nm. Los lagos estudiados, Moreno, El Trébol, Nahuel Huapi, Gutiérrez y Morenito, están ubicados la región Patagónica (41° S, 71° W, 800 m de altitud y tienen coeficientes de atenuación variables entre 0,36 m-1 (Lago Moreno y 2,8 m-1 (Lago Morenito. Las muestras se inocularon con carbono marcado (NaH14CO3 y se incubaron en una cámara iluminada (UV-B = 0,35 W m-2, UV-A [320-400 nm] = 1,1 W m-2, y PAR [400-700 nm] = 10,8 W m-2 a 10° C. El fitoplancton se expuso a PAR (radiación fotosintéticamente activa + radiación UV (tubos de cuarzo, y a PAR + UV-A (tubos de cuarzo recubiertos con Mylar-D. La duración total de los experimentos fue de 4 h y se retiraron dos muestras de cada tratamiento cada 1 h. En los Lagos Moreno, El Trébol, Nahuel Huapi y Gutiérrez la inhibición fotosintética aumentó en forma linear con la dosis de UV-B, mientras que en el Lago Morenito esta relación fue débil. Después de recibir una dosis de 1,25 kJ m-2 (UV-B, el fitoplancton del Lago Morenito tuvo la mayor inhibición fotosintética acumulada (44 %, mientras que en los Lagos Moreno, Trébol, Nahuel Huapi y Gutiérrez la inhibición fue de 22, 11, 5 y 1 %, respectivamente. Sin embargo, al final de la incubación, y luego de recibir una dosis de 5 kJ m-2, el fitoplancton más inhibido fue aquel del Lago Moreno (70 %, y el más resistente aquel del Lago Gutiérrez (27 %. La cinética de inhibición fue distinta para cada lago, registrándose una mayor velocidad en los lagos más transparentes y con mayor proporción de células grandes. Los resultados sugieren que, ante un aumento de UV-B (e.g., producto de una disminución de ozono estratosférico, el microplancton de lagos más claros será el más afectado mientras que el pico- y nanoplancton de lagos menos transparentes será el más resistente

  11. AS PERDAS TERRITORIAIS DO ESTADO BOLIVIANO (1825-1935

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    Fernando Siliano Reyes

    2009-12-01

    Full Text Available The present study approaches the constitution of the Bolivian State and its territorial losses since its foundation in 1825 until the end of the Chaco War in 1935. Not only did such losses diminish the size of this Bolivian State’s territory but also restrained the country’s circulation perspectives. The country has tried to minimize its losses through international agreements on territorial use with exactly those neighbors which have taken part of its territory.To understand this process, the present study describes Bolivia’s populational and territorial formation, beginning with pre-Colombian peoples, mainly the Quechuas and the Aymaras, followed by the Spanish occupation, taking into account the fact that Bolivia has its own plentiful historicity, and is specifically inserted in the order of the world capital. Last but not least, this paper discusses the successive wars fought against three neighbor countries – Chile, Brazil and Paraguay – which resulted in significant territorial loss to the Bolivian State.

  12. CONDEPA Y UCS: El declive del neopopulismo boliviano

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    Salvador Romero Ballivián

    2003-01-01

    Full Text Available CONDEPA y UCS nacieron con las transformaciones de los años 1980 en Bolivia: transición a la democracia, crisis económica, aplicación del liberalismo económico, valoración del multiculturalismo. Integraron la tercera generación partidaria del siglo XX, caracterizada por su respeto a la democracia, la personalización de su liderazgo, su respuesta a la marginalidad social y a la pérdida de confianza en el nuevo proyecto estatal. CONDEPA y UCS alcanzaron su cenit en la presidencial de 1997. Sin embargo, cinco años después CONDEPA ocupó el último lugar y la UCS se convirtió meramente en un actor secundario. El artículo sigue el abrupto final de estos partidos, analizando los rasgos compartidos y las dificultades propias de cada organización: la personalización del liderazgo, el costo de la participación en la administración pública, la mala gestión de la herencia política y económica dejada por los fundadores

  13. Músicas, movimientos, colores en la fiesta andina: ejemplos bolivianos

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    Rosalía Martínez

    2014-01-01

    Full Text Available En la fiesta andina, la música suele tanto oírse como verse. Esta dimensión multisensorial de la situación musical no es únicamente el resultado de una yuxtaposición de elementos sonoros y visuales. El análisis de las articulaciones que los campesinos indígenas de la zona de sucre (Bolivia construyen entre sonidos, movimientos y colores revela la presencia de organizaciones singulares de la experiencia sensible que se caracterizan tanto por su espesor sensorial como por la manera en la cual se encuentran conectadas con otros campos del conocimiento. Estas formas de intersección culturalmente elaboradas implican el cuerpo mismo de los músicos, generando nuevas configuraciones perceptivas.

  14. Evidencias fotográfica, biológica y genética de la presencia actual de jaguaroundi (Puma yagouaroundi en Michoacán, México Photographic, biological and genetic evidences of the presence of jaguaroundi (Puma yagouaroundi at the moment in Michoacán, Mexico

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    Tiberio C. Monterrubio-Rico

    2012-09-01

    Full Text Available El jaguaroundi, a pesar de su amplia distribución neotropical, es uno de los felinos menos estudiados del continente y se carece de estudios genéticos sobre la especie. Para el estado de Michoacán ha existido la sospecha de su presencia y no obstante que sólo se tenía un registro del año 1970, los mapas de distribución de la especie en México incluyen al estado. Combinando métodos de campo (trampas cámara, recolección de campo, transectos y genotipificación molecular, obtuvimos evidencia fotográfica, biológica y genética que confirma la presencia actual de jaguaroundi (Puma yagouaroundi en 3 regiones del estado de Michoacán, México. Se obtuvieron 11 registros fotográficos en 7 localidades con bosque tropical y 7 de estos registros, revelaron que la especie está activa principalmente por la tarde, que existen 2 fases de pelaje, predominando la fase clara y que se reproduce en el estado. Con base en las distancias e independencia entre registros de los municipios de Arteaga y Lázaro Cárdenas, se plantea la hipótesis de que la distribución continúa a lo largo de la sierra Madre del Sur y la costa del Pacífico de Michoacán, aunque se desconoce si hay conectividad hacia la depresión del Balsas. Se obtuvieron 2 secuencias de 1089 y 1096 pb del gen de citocromo b que actualmente son las más largas que se han obtenido para la especie en México y el norte del continente. Las secuencias indican que hay 2 haplotipos distintos. La presencia de la especie en 3 regiones y los 2 haplotipos permiten suponer que en Michoacán puede contar con importante diversidad genética, aunque hace falta ampliar el tamaño de muestra para confirmarlo. Las secuencias obtenidas permitirán la comparación con individuos de otras regiones del país para conocer mejor la variabilidad genética en la especie y auxiliarán en la identificación de poblaciones para conservación.The jaguaroundi is one of the least studied felids on the American

  15. NOXA、PUMA等基因在乳腺癌中的表达及其意义%The Expression Characteristics of MDM2, PCNA, NOXA and PUMA in Breast Cancer

    Institute of Scientific and Technical Information of China (English)

    王尚文; 佟钧; 诸晨; 平妮娜; 喻箴; 崔明; 何越峰

    2016-01-01

    目的 比较癌组织与正常组织中MDM2、PCNA、NOXA、PUMA表达的差异,了解乳腺癌与表达的关系.方法 通过SYBR Green实时荧光定量PCR方法检测乳腺癌组织和正常乳腺组织中MDM2、PCNA、NOXA、PUMA 4个基因的含量.结果 (1) NOXA、和PUMA在乳腺癌组织中表达升高(P<0.05);(2) PCNA和NOXA的表达存在正相关关系(r=0.82,P<0.05));PCNA和MDM2的表达存在正相关关系(r=0.80,P<0.05);PUMA和MDM2的表达存在正相关关系(r=0.82,P<0.05).结论 NOXA、PUMA在乳腺癌组织中处于高表达状态,4个基因表达与乳腺癌有关.

  16. El frente de corrimiento andino al nivel de los cerros Penitentes y Visera (alta Cordillera de Mendoza: aspectos cronológicos y cartográficos The Andean thrust front in the Cerros Penitentes and Visera (High Cordillera of Mendoza: chronological and cartographic aspects

    Directory of Open Access Journals (Sweden)

    Jean-Claude Vicente

    2009-10-01

    Full Text Available El hallazgo de una iportante fauna de invertebrados marinos del Tithoniano superior a espaldas del cerro Penitentes en la secuencia carbonática basal de la primera escama del frente de corrimiento andino, confirma la lógica tectónica de un despegue de esta unidad a nivel del Yeso Principal y lleva a invalidar ciertas correlaciones litoestratigráficas y bosquejos paleogeográficos asignados a la Formación La Manga. Por otro lado, el análisis de algunos perfiles claves en torno al cerro Penitentes entre la quebrada Vargas y el cerro Visera permite presentar una reinterpretación tectónica del sector. Esta se sustenta en el reconocimiento y la diferenciación de las unidades terciarias básicas representadas por los Conglomerados Penitentes (Mioceno inferior y los Aglomerados Santa María (Mioceno superior en una relación muy similar a la descrita anteriormente al norte de Puente del Inca. El estudio permite seguir las interferencias, en un contexto polifásico, entre las reactivaciones del corrimiento Penitentes de piel fina y un cabalgamiento frontal de piel gruesa que repite la secuencia terciaria. La discordancia entre las secuencias terciarias y el emplazamiento de una klippe de Malm sobre los Conglomerados Penitentes del sinclinal del cerro Visera, conduce a otorgar cierta importancia a la fase del final del Mioceno inferior.The finding of an important marine invertebrate fauna of Upper Tithonian age at the shoulders of the Penitentes hill, in the basal carbonatic sequence of the first Andean wedge confirms the tectonic logic of a detachment of this unit at the level of the Yeso Principal. This allows invalidating certain lithostratigraphic correlations and paleogeographic sketches ascribed to the La Manga Formation. On the other hand, the analysis of some key sections around the Penitentes hill area between the Quebrada de Vargas and the Cerro Visera, allows presenting a tectonic reinterpretation of the sector. This is based on the

  17. Evaluación de la eficiencia fotosintética del fitoplancton en un lago amazónico (Lago Boa y en un lago andino (Lago Guatavita Evaluation of the phytoplanktonic photosynthetic efficiency in an Amazon Lake (Lake Boa and in an Andean Lake (Lake Guatavita

    Directory of Open Access Journals (Sweden)

    Gabriel A. Pinilla

    2006-01-01

    Full Text Available La relación entre la fluorescencia variable y la fluorescencia máxima (Fv/Fm de las comunidades de algas se ha utilizado comúnmente como una medida de la eficiencia fotosintética del fitoplancton. Dicha eficiencia puede estar afectada por la localización de los ambientes acuáticos en distintas provincias limnológicas. En este trabajo se quiso establecer la diferencia en la relación Fv/Fm entre el lago Boa (150 msnm, Amazonía colombiana y el lago Guatavita (3000 msnm, cordillera oriental de los Andes colombianos. Los promedios de las eficiencias medidas fueron en general bajos (0,212 a 0,367 y 0,089 a 0,32 en los lagos Boa y Guatavita, respectivamente, lo que señala estrés fisiológico para las algas. La eficiencia fue mayor en aguas intermedias y presentó cambios fuertes entre épocas de muestreo y entre ecosistemas. En aguas superficiales se presentó fotoinhibición, la cual fue más fuerte en Guatavita. La eficiencia fotosintética fue menor en el lago andino debido posiblemente a diferencias climáticas, de altura sobre el nivel del mar y de estratificación. Durante los ensayos de laboratorio se observó que la eficiencia disminuyó con el tiempo, lo cual pudo deberse a una aclimatación de las algas a la oscuridad. Los datos de laboratorio confirmaron que el fitoplancton epilimnético del lago Guatavita estuvo fotoinhibido en la época de estratificación.Commonly relation between variable fluorescence and maximum fluorescence (Fv/Fm of the algae community has been utilized like a measure of the phytoplanktonic photosynthetic efficiency. Such efficiency can be affected by the water bodies' location in different limnological provinces. The goal of this work was to establish the differences in the Fv/Fm rate between Lake Boa (150 masl Colombian Amazon and Lake Guatavita (3000 masl, East Range in the Colombian Andes. In general, averages of the photosynthetic efficiencies were low (0.212 to 0.367 and 0.089 to 0.32 in lakes Boa and

  18. Fetal and postnatal pulmonary circulation in the Alto Andino.

    Science.gov (United States)

    Llanos, A J; Ebensperger, G; Herrera, E A; Reyes, R V; Pulgar, V M; Serón-Ferré, M; Díaz, M; Parer, J T; Giussani, D A; Moraga, F A; Riquelme, R A

    2011-03-01

    Lowland mammals at high altitude constrict the pulmonary vessels, augmenting vascular resistance and developing pulmonary arterial hypertension. In contrast, highland mammals, like the llama, do not present pulmonary arterial hypertension. Using wire myography, we studied the sensitivity to norepinephrine (NE) and NO of small pulmonary arteries of fetal llamas and sheep at high altitudes. The sensitivity of the contractile responses to NE was decreased whereas the relaxation sensitivity to NO was augmented in the llama fetus compared to the sheep fetus. Altogether these data show that the fetal llama has a lower sensitivity to a vasoconstrictor (NE) and a higher sensitivity to a vasodilator (NO), than the fetal sheep, consistent with a lower pulmonary arterial pressure found in the neonatal llama in the Andean altiplano. Additionally, we investigated carbon monoxide (CO) in the pulmonary circulation in lowland and highland newborn sheep and llamas. Pulmonary arterial pressure was augmented in neonatal sheep but not in llamas. These sheep had reduced soluble guanylate cyclase and heme oxygenase expression and CO production than at lowland. In contrast, neonatal llamas increased markedly pulmonary CO production and HO expression at high altitude. Thus, enhanced pulmonary CO protects against pulmonary hypertension in the highland neonate. Further, we compared pulmonary vascular responses to acute hypoxia in the adult llama versus the adult sheep. The rise in pulmonary arterial pressure was more marked in the sheep than in the llama. The llama pulmonary dilator strategy may provide insights into new treatments for pulmonary arterial hypertension of the neonate and adult.

  19. Los pasos del folklore colombiano. El folklore andino

    Directory of Open Access Journals (Sweden)

    Manuel Zapata Olivella

    1961-03-01

    Full Text Available El Bambuco y la Güabina representan el folklore de los Andes colombianos. Tres elementos muy definidos les caracterizan: la acentuada influencia española, la melodía y la copla inspirada. La raíz española la denuncian la guitarra y el tiple, entre los instrumentos; la danza, que no solo les asemeja a muchos bailes peninsulares, sino que los emparenta con la cueca chilena; el punto guanacasteco costarricense; el joropo venezolano; el huapango de México y en general, con el folklore mestizo de toda Hispanoamérica y por último, el acento hispano de la copla picaresca.

  20. Representaciones del Mundo Andino en un relato oral sobre fantasmas.

    Directory of Open Access Journals (Sweden)

    Florencia Raquel Angulo Villán

    2010-11-01

    Full Text Available Este trabajo centra el análisis en las narraciones de tradición oral que tematizan las historias de fantasmas. Si bien existen “constelaciones de imágenes” más o menos constantes compartidas por el ser humano y su entorno material y social, éstas se enriquecen en tanto pueden ser leídas desde los códigos de la cultura que las produce. En este caso, representada por la zona andina. La metodología utilizada pondrá en relación las categorías de imaginario desde una perspectiva antropológica y los aportes de la filosofía andina, sustentada en los principios de reciprocidad y complementariedad. La lectura analítica tiene como objetivo poner de manifiesto cómo los discursos orales que circulan en una comunidad en particular se constituyen como estrategias de consolidación de la “memoria” de un pueblo.

  1. Relaciones comerciales Pacto Andino-España

    Directory of Open Access Journals (Sweden)

    Adelaida Checa Sánchez

    1993-01-01

    Full Text Available La integración de varios países en todos los aspectos, pero principalmente en el económico, es un fenómeno mundial propio de la segunda mitad del siglo xx, y que cada vez se extiende con más fuerza, tanto entre los países desarrollados, como entre los del tercer mundo. La CE. es el prototipo de todas las integraciones existentes hasta el presente, y la más completa, aunque se encuentra todavía en un período de transición. Hacia ella vuelven sus ojos la mayor parte de los grupos de países iberoamericanos que se integran en el aspecto económico, pero con deseos de llegar a una unión más fuerte, que les permita un mayor y rápido desarrollo.

  2. Isolation and genetic characterisation of Toxoplasma gondii from a red-handed howler monkey (Alouatta belzebul), a jaguarundi (Puma yagouaroundi), and a black-eared opossum (Didelphis aurita) from Brazil.

    Science.gov (United States)

    Pena, H F J; Marvulo, M F V; Horta, M C; Silva, M A; Silva, J C R; Siqueira, D B; Lima, P-A C P; Vitaliano, S N; Gennari, S M

    2011-02-10

    Toxoplasma gondii isolates are highly diverse in domestic animals from Brazil. However, little is known about the genetics of this parasite from wild mammals in the same region. Reveal genetic similarity or difference of T. gondii among different animal populations is necessary for us to understand transmission of this parasite. Here we reported isolation and genetic characterisation of three T. gondii isolates from wild animals in Brazil. The parasite was isolated by bioassay in mice from tissues of a young male red handed howler monkey (Alouatta belzebul), an adult male jaguarundi (Puma yagouaroundi), and an adult female black-eared opossum (Didelphis aurita). The monkey and the jaguarundi had inhabited the Zoo of Parque Estadual Dois Irmãos, Pernambuco State, Northeastern Brazil, for 1 year and 8 years, respectively. The wild black-eared opossum was captured in São Paulo State, Southeastern Brazil, and euthanised for this study because it was seropositive for T. gondii (titre 1:100 by the modified agglutination test, MAT). Ten PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism) markers, SAG1, SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1 and Apico, were used to genotype the isolates. T. gondii was isolated from the brain and heart homogenate of the monkey, the muscle homogenate of the jaguarundi, and the heart homogenate of the black-eared opossum. This was the first isolation of T. gondii from a neotropical felid from Brazil. The isolate from the monkey (TgRhHmBr1) was not virulent in mice, whereas the isolates from the jaguarundi (TgJagBr1) and the black-eared opossum (TgOpBr1) were virulent in mice. The genotype of the isolate from the monkey has been identified in isolates from a goat and ten chickens in the same region of Brazil, suggesting that it may be a common lineage circulating in this region. The genotypes of the isolates from the jaguarundi and the black-eared opossum have not been previously reported. Although there

  3. Geología de la región del Río Bonete en el antepaís andino (27º30'LS: extremo norte del Terreno de Precordillera Geology of the region of Rio Bonete: Northern edge of the Precordillera terrane

    Directory of Open Access Journals (Sweden)

    Federico Martina

    2009-04-01

    Full Text Available En la región del antepaís andino en la provincia de La Rioja (28º31'S - 68º30'W aflora una faja de deformación dúctil con orientación NW, parcialmente expuesta en el río Bonete, conocida con el nombre de faja de deformación de Jagüé. Trabajos previos sugieren que constituiría el límite norte del terreno de Precordillera. Está fundamentalmente compuesta por ortoanfibolitas intensamente foliadas que gradan a gabros, granitos milonitizados y ultra-milonitizados de edad grenviliana y lentes de mármol bandeado y de grano fino, cálcicos y dolomíticos. El conjunto fue tradicionalmente incluido dentro de la Formación Río Bonete, separándose a los mármoles como Miembro Caliza Las Damas. La Formación Río Bonete incluía, además, una sucesión de areniscas y pelitas con arreglo rítmico y afectado por muy bajo grado metamórfico de edad ordovícica superior. De acuerdo a un nuevo mapeo, se trataría de dos unidades distintas separadas por discordancia, considerando conveniente utilizar el término Complejo Metamórfico Río Bonete para la unidad con deformación dúctil y Formación Chuscho para las metasedimentitas interdigitadas con vulcanitas básicas incluidas en la faja ofiolítica famatiniana. Por su parte, y para evitar confusiones, se propone reemplazar el término Miembro Calizas Las Damas por el de Mármol Las Damas que refleja mejor su composición. Considerando que la Formación Chuscho, a pesar de su muy bajo grado de metamorfismo, no ha sido involucrada en la deformación dúctil y que los mármoles por su bandeado composicional se correlacionarían con unidades cámbricas de la plataforma del terreno de Precordillera, la edad de la faja quedaría acotada al Paleozoico inferior, consistente con la orogenia oclóyica. Análisis estructurales permiten interpretar una cinemática sinistral a normal para esta cizalla dúctil que, desde el punto de vista regional, se vincularía con el emplazamiento del terreno de

  4. PUMA: The Positional Update and Matching Algorithm

    Science.gov (United States)

    Line, J. L. B.; Webster, R. L.; Pindor, B.; Mitchell, D. A.; Trott, C. M.

    2017-01-01

    We present new software to cross-match low-frequency radio catalogues: the Positional Update and Matching Algorithm. The Positional Update and Matching Algorithm combines a positional Bayesian probabilistic approach with spectral matching criteria, allowing for confusing sources in the matching process. We go on to create a radio sky model using Positional Update and Matching Algorithm based on the Murchison Widefield Array Commissioning Survey, and are able to automatically cross-match 98.5% of sources. Using the characteristics of this sky model, we create simple simulated mock catalogues on which to test the Positional Update and Matching Algorithm, and find that Positional Update and Matching Algorithm can reliably find the correct spectral indices of sources, along with being able to recover ionospheric offsets. Finally, we use this sky model to calibrate and remove foreground sources from simulated interferometric data, generated using OSKAR (the Oxford University visibility generator). We demonstrate that there is a substantial improvement in foreground source removal when using higher frequency and higher resolution source positions, even when correcting positions by an average of 0.3 arcmin given a synthesised beam-width of 2.3 arcmin.

  5. I AM 60——PUMA

    Institute of Scientific and Technical Information of China (English)

    Vincnet

    2009-01-01

    PUMA的60大寿.有三个不同的意义。简而言之.PUMA这攻特别将这个60周年企划划分成40至50、60至70、80至90年代三个以不同年代背景为设计概念,继而转化成3个其不同年代的特殊系列。总体上.整个系列以不同色调贯穿3个不同年代背景,彤成强烈的对比,Unlimited Hi与Easv Rider球鞋是本系列的重点。而wndbreaker同样不可错过。

  6. Las lluvias en los Andes tropicales y el ENSO (El Niño Southern Oscillation)

    OpenAIRE

    Ronchail, Josyane

    2013-01-01

    Pronto cumplirán veinte años los estudios pioneros de Thompson et al. (1984) y de Francou & Pizarro (1985) que hacían mención del vínculo entre El Niño y la sequía en el altiplano peruano-boliviano. Alentada por ti, Olivier, devine investigadora del Instituto Francés de Estudios Andinos y se me encargó profundizar este tema. Los hidrólogos del Instituto de Investigación para el Desarrollo (IRD) me acogieron en La Paz en donde pasé dos años descubriendo un clima tropical y trabajando sobre… ¡l...

  7. Rethinking the recent past: Policies of the difference and interculturality

    Directory of Open Access Journals (Sweden)

    Fernando Garcés V.

    2008-12-01

    Full Text Available Desde la década del noventa del siglo pasado se hicieron explícitas unas políticas de la diferencia en los espacios estatales bolivianos (Postero, 2005;Paz, 2005a, 2006.1 Cuando hablamos de políticas de la diferencia nos referimos a la implementación de mecanismos de reconocimiento y tolerancia de la diversidad, cada vez más frecuentes al interior de los Estados del ámbito andino. Estos mecanismos de reconocimiento y tolerancia de la diversidad pueden esconder sutiles mecanismos de dominación bajo un discurso y unas prácticas incorporadoras y asimiladoras de lo diverso en el aparato estatal y en los circuitos de acumulación del capital.2 Se trata de unas políticas que pueden ser encontradas no sólo en declaraciones

  8. Probing HER2-PUMA and EGFR-PUMA Crosstalks in Aggressive Breast Cancer

    Science.gov (United States)

    2014-09-01

    have high resistance to p53 inducible therapies such as DNA damaging agents, UV, and gamma irradiation among others [19]. However, a number of...Following washes, protein A agarose pellets were dephosphorylated with recombi nant human PTP1B followed by incubation with recombinant human HER2 as

  9. El "Tío" está sordo: Los mineros bolivianos y el Patrimonio Cultural Inmaterial

    OpenAIRE

    Gerardo Fernández Juárez

    2013-01-01

    Los mineros quechuas de Potosí rinden culto en el interior de mina a un personaje al que denominan “Tío”, el diablo. El artículo reflexiona sobre las contradicciones aparentes en que incurre la UNESCO en el reconocimiento de los bienes de interés cultural intangible o inmaterial aplicadas, en este caso, a la figura del “Tío” y su contexto minero. Las perspectivas “emic” y “etic” chocan con especial violencia intelectual al valorar la significación cultural del “Tio” en las minas potos...

  10. Moviéndose por Argentina: Sobre la presencia de bolivianos en Ushuaia

    Directory of Open Access Journals (Sweden)

    Ana Inés MALLIMACI BARRAL

    2012-01-01

    presentan algunos resultados sobre el tipo de trayectorias migratorias experimentadas por las/os bolivianas/os y los mecanismos que operaron detrás de sus modalidades de asentamiento. Se demostrará que la presencia de bolivianas/os en Ushuaia responde tanto a lógicas locales, de los Estados y los mercados regionales, como a mecanismos propios de la migración boliviana en Argentina.

  11. Grupos marginados en el desarrollo político argentino-boliviano 1880-1920

    OpenAIRE

    2016-01-01

    Comparar el desarrollo político de Argentina y Bolivia no presenta mayores dificultades, por el contrario, con excepción del Brasil, quizá sean las realidades más opuestas entre todos los países hispanoamericanos, lo cual da un margen de comparación y contraste muy grandes. Sin embargo, el presente trabajo no tiene por objeto únicamente comparar ambas realidades sino más bien enfocar las perspectivas teórico metodológicas que se emplearon para estudiarlas. Se pretende establecer, sobre todo, ...

  12. A formação do movimento Katarista. classe e cultura nos Andes bolivianos

    OpenAIRE

    Mauricio Hiroaki Hashizume

    2010-01-01

    O protagonismo social de camponeses e indígenas na Bolívia é comumente associado à particular composição étnico-cultural da população do país vizinho. O exame do katarismo - nome herdado do índio insurgente Tupac Katari, que liderou rebelião contra os colonizadores espanhóis no final do século XVIII -, especialmente em sua fase inicial (1969 a 1985), permite uma compreensão mais ampla e complexa do processo de formação, mudança e consolidação da classe trabalhadora boliviana. Antes disso, o t...

  13. Educação ambiental e a conservação da onça parda: potenciais das unidades de conservação do nordeste paulista. Environmental education and puma conservation: potentials of the protected areas of northeastern São Paulo state (Brazil.

    Directory of Open Access Journals (Sweden)

    Mayla Willk VALENTI

    2014-06-01

    Full Text Available No nordeste do Estado de São Paulo as unidades de conservação abrigam importante parcela da biodiversidade brasileira. Espécies predadoras de topo de cadeia, como a onça parda (Puma concolor, ainda estão presentes nessa região e dependem dessas unidades para sobreviverem. Nesse contexto, a educação ambiental pode contribuir com a conservação das espécies, embora existam desafios a serem superados. O objetivo deste trabalho foi identificar potencialidades e limites da educação ambiental para a conservação da biodiversidade nas unidades de conservação do nordeste paulista, tendo como tema a conservação de predadores, em especial a onça parda. A área de estudo foi definida com base na população dessa espécie na região. Realizamos levantamento das unidades de conservação na área de estudo e consulta para identificar aquelas com programas de educação ambiental ativos. Visitamos sete áreas protegidas, sendo que em cada uma realizamos entrevista estruturada com pessoas envolvidas em ações de educação ambiental e/ou gestores das unidades, totalizando sete entrevistas. Os dados indicaram que essas unidades estão em contextos relevantes para a realização de ações educativas voltadas à conservação de predadores. Contudo, as/os entrevistadas/os relataram dificuldades em se aproximar da população do entorno fora do ambiente escolar, especialmente o público adulto. Apesar disso, identificamos uma preocupação em envolver a comunidade do entorno, percebida nos próprios objetivos das atividades realizadas pelas unidades de conservação. A partir dos resultados obtidos, consideramos que, embora o tema dos predadores não seja especificamente trabalhado, existe um potencial grande para atuação nesse sentido. Ainda, destacamos que as unidades de conservação são espaços privilegiados para a integração dos conhecimentos científicos e educação ambiental. In northeastearn São Paulo state (Brazil, the

  14. Comparación de métodos analíticos para la determinación de materia orgánica en suelos de la región Andino-Patagónica: efectos de la vegetación y el tipo de suelo Comparison of analytical methods for determining soil organic matter in Patagonian Andean Region: effects of vegetation and soil types

    Directory of Open Access Journals (Sweden)

    Ludmila La Manna

    2007-12-01

    Full Text Available La determinación de la materia orgánica (MO resulta fundamental para el conocimiento de la productividad agrícola y forestal de los suelos. En este estudio se evaluó la relación entre los contenidos de materia orgánica (MO determinados por pérdida por ignición (MO PI y combustión húmeda de Walkley-Black (MO CH en suelos de la Región Andino Patagónica. La relación se evaluó para suelos volcánicos con y sin aluminosilicatos amorfos y con distintos tipos de vegetación: plantaciones de Pinus ponderosa, bosques de Austrocedrus chilensis, arbustales, estepa arbustiva y estepa herbácea. Se seleccionaron 100 sitios de muestreo, donde se tomaron muestras compuestas del horizonte A para la determinación de MO CH y MO PI. Los datos fueron analizados mediante análisis de varianza y regresiones simples. MO CH fue siempre inferior a MO PI. Esto es esperable dado que MO PI incluye la MO total, mientras que MO CH discrimina las formas de carbono fuertemente condensadas. Si bien no se detectaron diferencias en la relación entre los métodos analíticos para suelos con y sin aluminosilicatos amorfos, sí existió una fuerte relación entre la presencia de estos y los contenidos absolutos de MO. La relación entre los métodos analíticos varió según el tipo de vegetación. Los suelos que sustentan vegetación de estepa herbácea y plantación de pino ponderosa presentaron las mayores diferencias entre los dos métodos analíticos. MO CH fue, en promedio, un 37% inferior a MO PI para estos tipos de vegetación, siendo significativamente superior a lo hallado en arbustales (26%. Los suelos con bosque denso de Austrocedrus chilensis y estepa arbustiva presentaron valores intermedios (30 y 35%, respectivamente. Las plantaciones de pino ponderosa (primera rotación, edad promedio 21 años fueron realizadas en áreas de estepa herbácea. Las similitudes encontradas entre ambos suelos podrían estar asociadas a características de la MO propias del

  15. PUMA基因转染胰腺癌AsPC-1细胞增强对5-FU致凋亡的敏感性%PUMA gene transfection increases sensitivity of pancreatic cancer cell line AsPC-1 to 5-FU-induced apoptosis

    Institute of Scientific and Technical Information of China (English)

    张克君; 李德春; 朱东明

    2008-01-01

    目的:探讨PUMA基因转染是否增强胰腺癌AsPC-1细胞对5-FU致凋亡的敏感性.方法: 采用脂质体转染法将PUMA-pCEP4和空载体pCEP4质粒转染入胰腺癌AsPC-1细胞中,G418筛选阳性细胞.将系列浓度(0.01~100 μmol/L)的5-FU 分别作用于AsPC-1、AsPC-1/PUMA和AsPC-1/pCEP4细胞72 h,MTT法测定各组细胞的存活率并计算IC50, FCM、断裂DNA琼脂糖凝胶电泳和TUNEL法检测细胞凋亡情况,Western blotting检测各组细胞PUMA蛋白表达的变化.结果: AsPC-1、AsPC-1/PUMA和AsPC-1/pCEP4细胞的5-FU IC50分别为(12±1.9)、(1.6±0 4)和(10.4±1.6)μmol/L,AsPC-1/PUMA细胞对5-FU作用的敏感性增加了7.5倍.5-FU以剂量依赖方式诱导AsPC-1细胞凋亡,但对AsPC-1/PUMA细胞所诱导的凋亡比AsPC-1和AsPC-1/pCEP4 更明显.低浓度5-FU(0.1 μmol/L)轻微引起AsPC-1/pCEP4[(1.14±0.28)%]和AsPC-1细胞凋亡[(0.9±0.23)%],但能诱导AsPC-1/PUMA细胞明显凋亡[(6.47±1.42)%];高浓度5-FU (1μmol/L)诱导各组细胞凋亡,但AsPC-1/PUMA细胞凋亡率[(34.54±9.36)%]明显高于AsPC-1[(12.8±3.74)%]和AsPC-1/pCEP4细胞[(15.8±5.15)%],差异均有统计学意义(P<0.01);FCM、断裂DNA琼脂糖凝胶电泳和TUNEL方法检测显示相同的结果.PUMA蛋白在AsPC-1/PUMA细胞中的表达明显高于AsPC-1和AsPC-1/pCEP4细胞.结论: PUMA基因转染明显地增强了胰腺癌AsPC-1细胞对5-FU致凋亡作用的敏感性.

  16. Pumas and Prepositions: Training Nature Guides in the Yucatan Peninsula.

    Science.gov (United States)

    Brown, Cynthia

    2000-01-01

    In the Yucatan Peninsula (Mexico), the 10-week Nature Guide Training Program integrates English immersion with natural history and environmental interpretation to train rural adults as nature guides and conservation leaders. Most graduates have found work as ecotourism guides or in conservation-related activities, and many have provided English…

  17. PUMA CELL VOLTRA跑步新“蜂巢”

    Institute of Scientific and Technical Information of China (English)

    SEXXXXXY

    2009-01-01

    PUMA的CELL蜂巢减震技术一直与时俱进地与PUMA跑鞋系列不断进步。从最原始的CELL,到双层的DUOCELL,再到07年的SPIDERCELL,以及去年用在CELL MEIO上的反转DUOCELL。如今,PUMA借新款跑鞋CELL VOLTRA推出了全新的蜂巢减震技术——10CELL。

  18. Buen vivir, Sumak Kawsay: Aporte contrahegemónico del proceso andino

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    Francisco Hidalgo Flor

    2011-01-01

    Full Text Available Este ensayo se propone mirar las evoluciones sociales y políticas de la región andina, inscritas en las demandas por procesos constituyentes y la elaboración de nuevas constituciones en Ecuador y Bolivia, uno de cuyos aportes profundos está en que ambas recuperan el concepto, de los pueblos indígenas quechuas, del Sumak Kawsay, y a partir de ello, la formulación del Buen Vivir, como horizonte de transformación, representando un aporte concreto en la lucha contrahegemómica, en la primera década del siglo XXI

  19. Constitución, Tratado de Libre Comercio Andino – Estados Unidos, Comunidad Andina

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    Marcel Tangarife Torres

    2007-12-01

    Full Text Available El presente escrito pretende hacer algunas reflexiones acerca de las relaciones jurídicas existentes entre la Constitución Política, el Tratado de Libre Comercio con Estados Unidos (TLC, y el ordenamiento jurídico de la comunidad andina. En esta oportunidad se recogerán algunos planteamientos hechos por el autor en publicaciones anteriores, con las actualizaciones, ampliaciones y precisiones que surgen de un proceso tan dinámico como el de la internacionalización de las relaciones económicas y comerciales. el autor pide excusas por citar algunos trabajos suyos, pues existe un gran vacío de doctrina especializada sobre la materia, vacío que ojalá sea llenado con prontitud.

  20. Apuntes sobre las relaciones entre el pensamiento, la lengua y la cultura, en el caso andino

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    Marie-France Souffez

    1984-12-01

    Full Text Available Este artículo reproduce algunas ideas generales sobre los problemas del sistema educativo peruano frente a la cultura andina. Avanza unas propuestas de investigación y desarrolla una posición teórica que subraya, al lado de los diferentes estudios sobre el hombre en su generalidad y permanencia,ia importancia de explorar las variaciones del espíritu humano en las diferentes culturas y épocas, lo que podría ser útil en. el Perú, país pluricultural dominado por una sola, cultura. Asimismo, propone indagar, dentro de la especificidad andina, sobre las formas de pensar y de expresar la inteligencia valoradas en esta cultura, iniciando la aproximación por un análisis de los significados de las expresiones lingüisticas relativas a estas formas.

  1. Constitución, Tratado de Libre Comercio Andino – Estados Unidos, Comunidad Andina

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    Marcel Tangarife Torres

    2007-12-01

    Full Text Available El presente escrito pretende hacer algunas reflexiones acerca de las relaciones jurídicas existentes entre la Constitución Política, el Tratado de Libre Comercio con Estados Unidos (TLC

  2. Somatotipo en jóvenes deportistas amateurs del mundo andino

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    Bejarano, Ignacio

    2005-01-01

    Full Text Available La técnica del somatotipo, de uso fundamental en antropología y medicina deportiva, describe conjuntamente la forma y la composición corporal de un sujeto. Así, sus tres cifras consecutivas reflejan los niveles de adiposidad, robusticidad musculo-esquelética y linearidad respectivamente. Se analiza aquí el somatotipo de participantes en los VI Juegos de la Juventud Transandina (San Salvador de Jujuy, Marzo 2003. Los deportistas de ambos sexos y edades entre los 8 y 20 años (n = 276, procedieron de Argentina, Bolivia, Chile y Perú donde existen regiones ubicadas a diferentes niveles de altitud geográfica. Las modalidades de competición fueron Básquet (n = 66, Volley (n = 82 y Natación (n = 128. Utilizando el método de Heath-Carter se estimaron las componentes de endomorfía, mesomorfia y ectomorfía en cada disciplina. Los valores medios fueron respectivamente Básquet: 4.50- 3.29- 3.20, Voley: 4.27-3.56- 3.09 y Natación: 3.40- 4.17- 2.99 para varones y 3.87- 3.90- 2.56 para mujeres. Para conocer el grado de homogeneidad se calcularon los índices de dispersión del somatotipo (SDI, observándose la mayor variabilidad en natación, seguido de Básquet y Voley. Los resultados se contrastaron con los reportados por otros autores tanto en países latinoamericanos como en otros continentes. Para ello se utilizaron estadísticos apropiados, como las distancias altitudinales medias (SAD. Frente a las series de élite comparadas y para las tres disciplinas, los jóvenes analizados presentan una endomorfia significativamente mayor, siendo las diferencias menores para los dos componentes restantes.

  3. Glosario del curanderismo andino en el departamento de Piura. Perú

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    Mario Polia

    1988-12-01

    Full Text Available Con el presente glosario queremos brindar a la atención de los estudiosos de eurimderismo una recolección e interpretación de términos rituales y de uso común entre las comunidades andinas y los curanderos de la sierra del departamento de Piura.

  4. El simbolismo del piojo en el mundo andino. Boceto filológico

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    Marie-France Souffez

    1985-12-01

    Full Text Available El valor que se da al piojo varía  según los lugares y las culturas del mundo. En las zonas urbanas peruanas es  sinónimo de  suciedad y  pobreza. En las zonas rurales tradicionales andinas, nos parece estar asociado a la pobreza y a la riqueza, a la muerte y a la vida. Las modalidades-y los matices de expresión de estas asociaciones forman el objeto de nuestro estudio. El presente artículo desarrolla un análisis parcial del vocabulario quechua y aimara relacionado con este parásito.

  5. Efectos negativos de las plantas exóticas maderables sobre el bosque nativo andino

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    Fidel Antonio Gutiérrez

    2011-07-01

    Full Text Available Los abusos que el hombre ha cometido con la naturaleza han provocado que muchas áreas, que debían ser santuarios vegetales, padezcan el deterioro del bosque nativo, con el consiguiente daño ambiental, hídrico, de flora y fauna. No escapa a ello la Escuela Nacional de Carabineros, pues su cerro tutelar fue sometido a una plantación de árboles exóticos maderables, con la consabida tala del bosque nativo. Los daños en dicho cerro han provocado la implementación de una reforestación técnica, que permita devolver la dinámica propia a la vegetación nativa, lo cual debe dar como resultado un Plan Estratégico de Manejo Ambiental, para futuras aplicaciones.

  6. Elecciones, partidos políticos y democracia en los paises andinos

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    Gabriel MURILLO CASTAÑO

    2009-11-01

    Full Text Available RESUMEN: El proceso democrático en América Latina debe salvar dos escollos para cimentarse definitivamente. El primer obstáculo tiene que ver con la debilidad del sistema de partidos atacado por vicios y escisiones lo cual ha impedido la representación de la sociedad civil en la toma de decisiones gubernamentales. El segundo tropiezo que ha soportado el naciente proceso democrático de la última década es el atinente a la falta de acciones gubernativas de largo plazo que logren postular coherentemente derroteros claros y precisos para el pleno desarrollo de cada país. No obstante, a pesar de los inconvenientes y las amenazas que ensombrecen el panorama democrático de la subregión andina, es razonable pensar que la democracia se consolidará paulatinamente intentando conciliar el sistema de partidos con una sana gobernabilidad para que ambos elementos no giren como ruedas sueltas del engranaje de la participación ciudadana.ABSTRACT: The democratic process in Latin America must successfully confront to problems in order to achieve a definitive consolidation. The first obstacle stems from the weakness of party systems undermined by vices and divisions that have impeded the representation of civil society in the making of governmental decisions. The second problem facing the novel democratic process of the past decade is the lack of long-term government actions that achieve clear and precise gools for the full development of each country. Nevertheless, despite the difficulties and dangers that darken the horizons of the democratic process in the Andean subregion, it is reasonable to think that real democracy will slowly consolidate, making an effort to conciliate the party system with a healthy governability in such a way that neither element will spin off like a loose wheel in the face of citizen participation.

  7. Mirando a la Pachamama: globalización y territorio en el Tarapacá andino

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    Sergio Gonzu00E1lez Miranda

    2004-01-01

    Full Text Available Este artículo aborda la distancia cultural entre las poblaciones indígenas y la sociedad occidental desde la Colonia hasta nuestros días a través del concepto tiempo y espacio (devenir y territorio, empleando el concepto agua como un elemento vital y ritual. Se analizan las oportunidades y los riesgos que les plantea la globalización a las comunidades andinas, ofreciendo una propuesta, basada en la teoría de Sergio Boisier, que podría resolver el dilema entre globalización y desarrollo endógeno en la sociedad indígena. Se señala la importancia de la nueva geografía cultural, como disciplina, para entender la importancia del territorio en sociedades y culturas no occidentales como la andina.

  8. Inmigrantes andinos en Madrid: Sus danzas y sus músicas tradicionales

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    Lourdes Morales

    2007-01-01

    Full Text Available In our urban context we get in touch with people that have been forced to leave their countries under different circumstances in order to live in Spain. This article analyses their wish to maintain a group identity and how this identity is materialized by some individuals through traditional dance and music. The study is intended as a framework for subsequent educational investigations that relate that cultural reality with the situation in secondary schools where children of Latin American immigrants study.Nuestro entorno ciudadano inmediato nos pone en contacto diario con personas que por diferentes razones se han visto obligadas a dejar sus países de origen para instalarse en España. Este artículo analiza el deseo que surge en ellas de mantener una identidad de grupo, y cómo ésta se concreta en ciertos individuos mediante manifestaciones de danza y música popular tradicional. El estudio pretende servir de marco para ulteriores investigaciones educativas que vinculen esa realidad cultural con la situación de institutos de enseñanza secundaria en los que estudian hijos de inmigrantes latinoamericanos.

  9. La frontera boliviano-brasileña en construcción (principios del siglo XX. Los conflictos territoriales, fiscales y legales. La Columna Prestes en el territorio boliviano.

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    Eugenia Bridikhina

    2015-01-01

    Full Text Available En este artículo pretendemos contribuir al estudio de los temas de las fronteras a través de zoom out sobre un tramo extenso de la frontera Bolivia - Brasil entre las poblaciones de San Matías y Puerto Suárez en las primeras décadas del siglo XX. En el artículo se tocarán los temas sobre los procesos de redefinición regional de las zonas fronterizas, el desempeño de las autoridades centrales, regionales y locales, la violencia, relacionada con el robo del ganado, provocada por los individuos y bandas organizadas que afectaba a los pobladores, y, sobre todo, a los grupos indígenas como los más vulnerables. Asimismo, se reconstruye la desconocida historia del célebre movimiento militar brasileño encabezado por Luis Carlos Prestes o la columna Prestes que se encontraba exilado en el territorio de Bolivia.

  10. Las relaciones de pareja entre migrantes bolivianos/as en Madrid: ¿Cambios, resistencias o continuidades?

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    Marcela Tapia Ladino

    2011-09-01

    Full Text Available La migración internacional es un escenario propicio para estudiar los cambios y las permanencias de la relaciones de género patriarcal desde la perspectiva de quienes migran. El artículo que se presenta quiere avanzar hacia la comprensión del impacto del cruce de fronteras en las relaciones entre hombres y mujeres desde la consideración relacional de género. Se advierte que los sistemas de desigualdad social en origen y en destino se enlazan con el género para describir tránsitos hacia relaciones más democráticas o de mayor subordinación en la sociedad receptora. Los elementos que explican dichas trayectorias se relacionan con factores de nivel micro social como el tipo de relación de pareja predominante antes de migrar, el capital social de las personas, y de nivel macro como las condiciones laborales y jurídicas que encuentran lo/as migrantes en el contexto de recepción. En general se aprecia que la migración es, al mismo tiempo, una oportunidad para el cambio a pequeña escala y una forma de dar continuidad a las prácticas tradicionales de género entre migrantes. Las diferencias en cada caso se relacionan con el impacto acumulado de la migración que vincula factores de nivel micro y estructural

  11. Economía y política del modelo boliviano 2006-2014: evaluación preliminar

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    Guillermo Alexander Arévalo Luna

    2016-01-01

    Full Text Available Bolivia sufrió un profundo cambio socioeconómico y político en el periodo 2006-2014, pasó de un modelo de crecimiento liberal a uno de economía mixta, donde el Gobierno juega un papel muy relevante en la economía. El Estado tiene un control completo de los recursos económicos y principales ingresos de la industria. El excedente generado por los commodities contribuyó a la política de distribución de ingreso y además fue utilizado para la lucha contra la pobreza a través de una mejor educación y salud. El modelo macroeconómico fue exitoso: la economía boliviana creció a una tasa promedio de 4.6 % anual durante el periodo 2006–2014 y registró un superávit fiscal, un balance comercial positivo y un aumento en el volumen de reservas internacionales. Además, el país goza de estabilidad macroeconómica y baja inflación.

  12. Una perspectiva antropológica sobre la tecnología andina y explicación de la racionalidad del hombre andino: nota sobre runakunapka wsayninkupag rurasquankunaqa: la tecnología en el mundo andino

    OpenAIRE

    Shimada, Izumi

    2014-01-01

    Este es el primero de dos volúmenes de una antología sobre la tecnología andina, ambiciosa y ampliamente concebida, que abarca un largo período (prehistoria a la actualidad) , disciplinas (antropología, etnohistoria, geografía y matemáticas) y regiones geográficas de los Andes Centrales (predominantemente el Perú).

  13. EL TURISMO ALTERNATIVO COMO FACTOR DE CAMBIOS EN LA MOVILIDAD ESPACIAL EN LOS PAÍSES ANDINOS.

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    María Skoczek

    2000-12-01

    Full Text Available En los años 90 del siglo XX hemos podido observar la expansión espacial de áreas turísticas en casi todos lospaises del mundo. Una de las causas principales de este fenómeno la constituye el interés creciente por diferentes formas del turismo alternativo, cuyos partidarios buscan lugares nuevos, tranquilos, pero a la vez atractivos debido a su. “otredad” en relación con las áreas de su residencia cotidiana.

  14. Los Tratados de Libre Comercio impulsados por EUA como una respuesta al fracaso de DOHA. El caso Andino

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    Rubén Flores

    2009-12-01

    Full Text Available La vigencia de la división del trabajo imperante entre el norte y el sur, bajo el actual escenario de globalización económica, esta siendo modificada, por la a nueva tendencia en la que cualquier producto, (sea materia prima, manufacturado o servicio puede ser producido en muchos países y regiones gracias en primer lugar al descenso en los costos de transporte, de telecomunicaciones, laborales, ambientales, que configuran unas ventajas de localización; pero además, a que luego del fracaso de la OMC desde la Ronda del Milenio hasta la de Doha y el fallido intento de la construcción de la Zona de Libre Comercio de las Américas (ALCA, la estrategia comercial de los EUA se ha centrado en impulsar acuerdos bilaterales de nueva generación TLC cuyo objetivo es lograr avanzar en una marco normativo que le garantice trato nacional y no discriminatorio al capital productivo extranjero (inversión extranjera, la estandarización de las normas técnicas, fitosanitarias, la defensa de los derechos de propiedad, entre otras.____________________ABSTRACT:The work division that exists between North and South, in this stage of economic globalization, it’s being modified by the new world tendency in which any product (raw materials, manufactured products or services can be produced in many countries and regions, thanks to: in first place, the lowering of transportation , telecommunications, working and environmental costs, that create localization advantages; in second place, the breakdown of the WTO since the Millennium Round to Doha Round and the failure FTTA has made that the USA commercial strategy focus on Bilateral Agreements of the new generation such as the FTA, which proposes to advance in legal issues that guaranties not to discriminate the foreign investment, the standardization of technique and sanitary and phytosanitary measures and the intellectual property rights defence, among others.

  15. Tecnologías convergentes en los países andinos: El caso de la nanotecnología

    OpenAIRE

    Aguirre Bastos, Carlos; Mercado, Alejandro F.; Aguirre A., José Luis; Portugal E., Rigliana; Paredes C., Carlos F.; Aguilar, Tirza J.; Forero P., Clemente; Gómez Baquero, Fernando; Banda, Hugo; Jaramillo, Rocío; Vega Centeno, Máximo; Morales, Rosa; Piselli, Roberto; Gutarra E., Abel; Vessuri, Hebe

    2007-01-01

    Las tecnologías convergentes se refieren a la combinación sinérgica (en dúos, tríos o cuartetos) de varias tecnologías transformativas (a) nanociencia y nanotecnología; (b) biotecnología y biomedicina, incluyendo la ingeniería genética; (c) tecnología de la información, incluyendo comunicación y computación avanzada y (d) ciencias cognitivas, incluyendo la neurociencia cognitiva. EI rápido desarrollo de estas tecnologías y su impacto hace que las políticas públicas y los sistemas de gobernabi...

  16. LA NUEVA ECONOMÍA MUNDIAL Y LOS CAMBIOS ESTRUCTURALES DE AME'RICA LATINA Y EL GRUPO ANDINO

    OpenAIRE

    Herrera García, Beatriz; Facultad de Ciencias Contables, Universidad Nacional Mayor de San Marcos

    2014-01-01

    El proceso de "mundialización', de la economía Internacional, desde la posguerra hasta principios de los años 70, se estuvo regulando mayormente desde las propias economías nacionales con una conducción bastante autónoma de sus respectivos estados. Ya avanzada la larga fase depresiva de la economía mundial que comenzará en los albores de la década de los años 70, parece más claro que la producción transnacionalizada de bienes y servicios es la que dicta las reglas sometiendo a los estados a s...

  17. Uso nocturno de perchas en dos especies de Anolis (Squamata: Polychrotidae en un bosque Andino de Colombia

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    Claudia Molina Zuluaga

    2007-01-01

    Full Text Available En este estudio registramos el uso de sitios para dormir por Anolis "anoriensis" y Anolis mariarum en un bosque montano al norte de la Cordillera Central de Colombia, con el objetivo de examinar la ocurrencia de segregación espacial entre ellas. Además, estimamos la disponibilidad de perchas en ambos sitios. Anolis mariarum durmió con mayor frecuencia en arbustos (51% que en otros tipos de perchas (arboles, helechos, herbáceas, y lo hizo en proporción similar a su disponibilidad. Anolis "anoriensis" utilizó de manera diferencial los tipos de percha en los dos sitios muestreados, dependiendo de la presencia/ausencia de la otra especie. Esta variación en la selección de los arbustos por A. "anoriensis" sugiere la existencia de competencia entre ambas especies cuando ocurren sintópicamente.In this study we recorded the use of sleeping site by Anolis "anoriensis" and Anolis mariarum in two study sites located in the montane forest of the extreme northern of Cordillera Central, Colombia. We also tested the occurrence of spatial segregation between the two species. Furthermore, we estimated perch availability in both study sites. Anolis mariarum slept more frequently on shrubs (51% than other kinds of perches (ferns, herbs, trees, but did so in proportion to their availability. Anolis "anoriensis" used different kinds of perches in each site, depending on the presence/absence of A. mariarum. Such variation in response to shrubs as sleeping perches by A. "anoriensis" suggests the existence of competition between the species when they are in syntopy.

  18. Terapeutas tradicionales andinos en un contexto de cambio: el caso de Churcampa en el Perú

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    Julio Portocarrero

    Full Text Available Objetivos. Describir la diversidad de terapeutas tradicionales, sus vínculos con el sistema de salud oficial y los cambios en sus recursos terapéuticos, en dos comunidades rurales de los andes en Churcampa, región de Huancavelica, Perú. Materiales y métodos. Estudio cualitativo en el que se combinan tres técnicas etnográficas: entrevistas en profundidad, observaciones y acompañamientos a terapeutas tradicionales y personal de salud. Resultados. Se identificaron 58 terapeutas, clasificados como curanderos, parteros y hueseros. La mayoría de terapeutas entrevistados aprendió su labor siendo joven y muy pocos tuvieron un maestro o mentor. La gran mayoría señaló tener reparos para vincularse con los establecimientos de salud más allá de las referencias. No había un trabajo articulado entre personal de salud y terapeutas tradicionales. Sin embargo, cuando sucedía, el personal de salud prefería a aquellos terapeutas que tuvieran la disposición para adecuarse a las necesidades de los servicios de salud. Se encontró que buena parte de los terapeutas estaban cambiando sus prácticas debido a la influencia de las iglesias evangélicas. Ningún terapeuta contaba con discípulos para transmitir sus conocimientos y prácticas. Conclusiones. Existe una gran diversidad de terapeutas tradicionales. Esta diversidad viene disminuyendo, entre otros factores, por el avance de las iglesias evangélicas. Los terapeutas tradicionales refieren a algunos pacientes al establecimiento de salud; mientras que el personal de salud establece relaciones jerárquicas de trabajo con los terapeutas. Se requiere trabajar estas actitudes del personal de salud pues la base de un enfoque intercultural es el trabajo articulado y horizontal entre ambos sistemas

  19. Micromamiferos andinos holocenicos del sitio arqueologico Inca Cueva 5, Ju Juy, Argentina: tafonomia, zoogeografia y reconstruccion paleoambiental

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    Ortiz, P. E.

    2002-08-01

    Full Text Available A micromammal archaeological sequence (2120 f 120 to 780 f 100 years BP from Inca Cueva 5 site (23" 05' S - 65" 27' W; 3700 m.a.s.l., Jujuy Province, Argentina was studied. The following mammalian taxa were recorded: Order Didelphimorphia, Family Didelphidae, Thylamys cf. T. pallidior; Order Rodentia, Family Muridae, Akodon cf. A. albiventer, Andinomys edax, Auliscomys sublimis, Calomys lepidus, Neotomys ebriosus, Phyllotis sp.; Family Caviidae, Cavia cf. C. tschudii, Galea musteloides; Family Abrocomidae, Abrocoma cinerea; Family Chinchillidae, Lagidium viscacia; Family Octodontidae, Octodontomys gliroides. The taphonomy indicates that the sequence is an owl pellet accumulation inside the cave. The general landscape corresponds to the oriental border of the Puna steppe, with scattered shrub and low brush vegetation. Near the site there are small tree groups of Polylepis australis as well as small streams covered with grass and ciperaceous vegetation known as «vegas». Al1 the recorded taxa at Inca Cueva 5 exist today in Puna and Prepuna environments from Jujuy and Salta Provinces and southem Bolivia, excepting Cavia cf. C. tschudii. The modem records of this species in northem Argentina and southern Bolivia come from localities with high montane bunchgrass prairies and forests of the humid Yungas phytogeographical region. The presence of this species in the sequence suggests wetter climatic conditions on this region when the archaeological site was originated. Later drier conditions, caused probably by anthropic influence in the last 500 years, produced the retraction of Cavia tschudii to the moist eastern side of the Andes and its disappeareance of the Puna areas.El sitio arqueológico Inca Cueva 5 se encuentra en la provincia de Jujuy, Argentina, en el borde oriental de la región de la Puna (23" 05' S - 65" 27' W; 3700 m s n m y está fechado entre 2120 f 120 y 780 f 100 años radiocarbónicos AP. El estudio de una pequeña muestra de micromamíferos permitió reconocer la presencia de los siguientes taxa: Orden Didelphimorphia, Familia Didelphidae, Thylamys cf. T. pallidior; Orden Rodentia, Familia Muridae, Akodon cf. A. albiventer, Andinomys edax, Auliscomys sublimis, Calomys lepidus, Neotomys ebriosus, Phyllotis sp.; Familia Caviidae, Cavia cf. C. tschudii, Galea musteloides; Familia Abrocomidae, Abrocoma cinerea; Familia Chinchillidae, Lagidium viscacia; Familia Octodontidae, Octodontomys gliroides. La tafonomía indica que el agregado tuvo su origen en la depositación de egagrópilas de lechuza (Aves, Strigiformes en el contexto de la cueva. El ambiente general actual en la zona corresponde a la estepa puneña, con arbustos y matorrales bajos y dispersos. En el fondo del valle hay bosquecillos de Polylepis australis, un curso de agua y vegas pequeñas y circunscritas. Todas las especies registradas en Inca Cueva 5 habitan actualmente en ambientes de Puna y Prepuna de Salta, Jujuy y sur de Bolivia, a excepción de Cavia cf. C. tschudii. Los registros actuales de esta especie en el noroeste de Argentina y sur de Bolivia corresponden a localidades ubicadas en pastizales de altura y bosques subtropicales de Yungas. La presencia de este taxón en la secuencia sugiere condiciones de mayor humedad que las actuales para la región durante el lapso considerado. Condiciones posteriores de mayor aridez, probablemente asociadas al impacto antrópico de los últimos 500 años, habrían redundado en la desaparición de Cavia tschudii de áreas puneñas y en su retracción a los cordones orientales húmedos de los Andes.

  20. Reproducción identitaria y social en las redes translocales de migrantes de dos pueblos andinos

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    Jacques Malengreau

    2008-12-01

    Full Text Available Se analizan de manera comparativa —según su destino y según su origen— las estrategias adoptadas por los migrantes de dos comunidades andinas de distintas regiones del Perú en su manejo de recursos sociales, culturales y materiales de su entorno extendido en el cuadro de su experiencia translocal. Estas estrategias están fundadas en una forma paralela. Por una parte, sobre una extensión de los lazos domésticos del pueblo originario, sobre todo a través de una continuidad transgeneracional basada en la descendenciacolateral tío(a – sobrino (a entre el pueblo originario y el lugar de inmigración, lo que implica un debilitamiento de las identidades colectivas supradomésticas originarias. Y, por otra parte, sobre la construcción de lazos vecindarios nuevos interétnicos, lo cual reinventa un modelo de solidaridad social en el lugar de inmigración. Estas estrategias —que combinan la jerarquización, el clientelismo, así como la reciprocidad y la redistribución— implican una reinterpretaciónde las normas vehiculadas por el modelo empresarial «liberal»,productivista, competitivo y consumista acondicionado a partir de un mundo extranjero que se manifiesta en una forma transnacional y transregional. En consecuencia, se pueden ver de modo comparativo las estrategias seguidas por los migrantes de ambos pueblos originarios como un desarrollo, tanto de rasgos comunes, como de particularidades que son ligadas, no solamente a las experiencias sociohistóricas y a las herencias culturales propias de cada uno de los pueblos, sino también al tipo de destino escogido por ellos; porejemplo, Lima metropolitana, los centros urbanos regionales y los valles tropicales del oriente. -- This article contrasts strategies adopted by migrants from two Andeancommunities located in different regions in Peru, in their useof social, cultural, and material networks, taking into consideration the place they settle in and where they come from, their translocal experience. These strategies are embedded in a parallel form; on the one hand there is an extension of the domestic bonds from the original village in the place they finally settle down in, mostly givenby a transgenerational continuation, based on collateral descendance (uncle/aunt – nephew/niece, which weakens the original supradomestic collective identities; and, on the other hand, the newly interethnic neighborhood bonds which transform the social solidarity model in the place they settle down in. These strategies that combine hierarchy, clientelism, reciprocity and redistribution, also imply a new interpretation of norms regulated by the «liberal», productive, competitive and consumer model, conditioned by a foreign world which is manifested in a transnational and transregional form.Hence, these strategies can be seen as a development of common traits, particularities related not only to social-historic and cultural heritage, but also to a type of destination chosen by these migrants, for example metropolitan Lima, the urban and regional centers and the Oriental tropical valleys.

  1. Políticas de salud basadas en el concepto de interculturalidad: Los centros de salud intercultural en el altiplano boliviano

    Directory of Open Access Journals (Sweden)

    Susana Ramírez Hita

    2009-07-01

    Full Text Available Actualmente en Bolivia, uno de los mayores problemas en el ámbito de la salud es la incorporación del concepto de cultura a las políticas sanitarias, hecho que hizo que se crearan centros de salud denominados "interculturales", así como el Modelo de Salud Familiar Comunitario e Intercultural. Este artículo, a través de una investigación realizada en el año 2007 en la zona altiplánica de Bolivia, pretende demostrar como el concepto de cultura está sirviendo de base para implementar políticas que no están ayudando a cambiar los deficientes índices epidemiológicos del país.One of the most important problems in the health field nowadays in Bolivia is the incorporation of the culture concept to public health politics, which had led to the creation of health centers called "intercultural", as well as the Communitary and Intercultural Model of Family Health. This paper, based on a research project undertaken in 2007 in the Bolivian altiplano, seeks to demonstrate how the culture concept is being used as a basis to implement politics that are not contributing to improve the deficient epidemiological indexes of the country.

  2. La “defensa social”. Pugnas y competencias en torno a la mano de obra en el Oriente Boliviano, 1890-1910.

    OpenAIRE

    Ana María Lema Garrett

    2010-01-01

    A fines del siglo XIX, la explotación de los gomales ubicados en la región amazónica boliviana, al norte del país, suscitó profundos cambios en otros departamentos y, entre otras cuestiones, en el mercado laboral. Los departamentos de Beni y de Santa Cruz se constituyeron entonces en reservorios de mano de obra y el flujo migratorio hacia las regiones gomeras del norte fue creciendo proporcionalmente a las necesidades de los empresarios gomeros. Sin embargo, las regiones de origen, y espec...

  3. Producción de β-glucosidasas por cultivos de bacterias termófilas indígenas del altiplano boliviano

    Directory of Open Access Journals (Sweden)

    Esther Casablanca Alarcón

    2011-08-01

    Full Text Available β-glucoside production by thermophilic bacteria indigenous the bolivian altiplano culture Resumen Las β-glucosidasas son enzimas que poseen actividad hidrolítica y transferasa o transglucosidasa. Tienen diversas aplicaciones; en la biosíntesis de oligosacáridos, producción de etanol utilizando residuos agrícolas y en la industria de vinos. La aplicación industrial, sin embargo, requiere estabilidad a temperaturas elevadas, por lo que los microorganismos termófilos tienen gran interés. El propósito de esta investigación es el de optimizar el medio de cultivo anaerobio de bacterias termófilas, para aumentar la producción de β-glucosidasas. Esta enzima es producida por tres aislados bacterianos: FT3, 2B y P5 los cuales fueron aislados de la región andina de Bolivia. El aislado bacteriano FT3 mostró una actividad β-glucosidasa de 0,35 [UI/mL]. Se tomaron como variables dentro de la optimización del medio de cultivo las fuentes de nitrógeno y de carbono, y el pH. Así también se probaron dos sistemas de cultivo: células libres y encapsuladas. Empleando extracto de levadura como fuente de nitrógeno se obtuvo una actividad de 0,52 [UI/mL]. En la optimización del pH del medio de cultivo se obtuvo una actividad de 0,81 [UI/mL] a pH 5. Como fuente de carbono se eligieron los hidrolizados de paja de trigo y paja de quinoa llegándose a obtener actividades de 1,27 y 1,34 [UI/mL] respectivamente. Se estableció que la localización celular de la enzima β-glucosidasa es extracelular y presenta estabilidad hasta una temperatura de 80 °C y un pH de 7.Palabras clave: optimización, células inmobilizadas, bacterias extremófilas.  Abstract The β-glucosidases possess hydrolytic and transferase activity or transglucosidase. They have various applications; such as biosynthesis of oligosaccharides, production of ethanol using agricultural residues and wine industry. However for industrial application, stability to high temperatures is needed. Therefore a great interesting in the thermophile microorganism study exist. The purpose of this research is to optimize the culture medium of thermophilic anaerobic bacteria to increase the production of β-glucosidase. This enzyme is produced by three isolate bacterial FT3, 2B and P5 which were isolated from the Andean region of Bolivia. FT3 isolate showed β-glucosidase activity of 0.35 [IU/mL]. In regards to the optimization of culture medium variables such as nitrogen source, carbon source and pH were taken into account and also the combination with free and encapsulated bacterial cells. Yeast extract was the selected source of nitrogen obtaining an activity of 0.52 [IU/ mL]. The optimal pH was 5 obtaining an activity of 0.81 [IU/mL]. The selected carbon source was the hydrolyzed wheat straw and quinoa straw obtaining activities of 1.27 and 1.34 [IU/mL], respectively. The cellular localization of β-glucosidase enzyme is extracellular and provides stability to temperature of 80 °C and stability at pH 7.Key words: Optimization, immobilized cells, extremophile bacteria.

  4. 12. Evaluación del seguimiento y de la promoción del crecimiento realizados en los Centros de Salud bolivianos

    OpenAIRE

    Méjean, Caroline; Maire, Bernard; Sejas, Edgar; Suremain, Charles-Édouard de

    2015-01-01

    1. Introducción El Proyecto, centrado en la problemática general del crecimiento y desarrollo del niño pequeño, ha implementado una estrategia para sensibilizar y promover la participación de las madres en el cuidado de salud de los niños menores de cinco años. En Bolivia, la fase operativa del Proyecto consistió en la elaboración e introducción de un nuevo carnet de salud, el Manual de Salud Infantil (msi), concebido con el fin de mejorar las actividades de promoción del crecimiento (véase C...

  5. ¿Carga o bendición? La respuesta de organizaciones no gubernamentales y gobiernos locales bolivianos a la migración internacional

    NARCIS (Netherlands)

    Nijenhuis, G.; Bogaardt, I. van den; Weenink, G.

    2012-01-01

    La migración internacional forma parte de la vida cotidiana de muchos hogares en el Sur. A muchas personas les atraen las oportunidades de trabajo en otros países - tanto en el Sur como en el Norte - con el fin de ganarse la vida. Muchos de estos migrantes mantienen estrechos vínculos con sus famili

  6. Efectos de espacio sobre costos de provisión local. Un modelo de ecualización por bloques para los municipios bolivianos

    Directory of Open Access Journals (Sweden)

    Barrios Suvelza, Franz Xavier

    2005-12-01

    Full Text Available Bolivia experimentó una importante transformación territorial después de que se aprobó la Ley de Participación Popular en 1994. Los efectos benéficos de tal reforma estuvieron acompañados de una serie de efectos contraproducentes, entre ellos los que se derivan del modelo de transferencias centrales hacia los municipios. Este artículo propone un nuevo modelo de transferencias que complementa el actual ¿sistema de dotación fiscal a municipios según el principio per cápita¿ con uno de ¿ecualización fiscal intermunicipal por bloques¿.

  7. Impacts of the Bolivian regulatory changes for the future of natural gas in Brazil; Impacto na relacao Brasil-Bolivia com a nacionalizacao dos hidrocarbonetos bolivianos de 2006

    Energy Technology Data Exchange (ETDEWEB)

    Duarte, Berrnardo Pestana Mello C.; Saraiva, Thiago Carvalho; Bone, Rosemarie Broker [Universidade Federal do Rio de Janeiro (UFRJ), RJ (Brazil)

    2008-07-01

    This paper aims to clarify and understand the reasons for the current economic policy and trade between Bolivia and Brazil, the latter represented by PETROBRAS, as the year 2006. Focuses on the regulatory change in Bolivia that culminated in a period of re-nationalization of its mineral wealth as opposed to international interests, including Brazilians, and agreements entered into during the opening of the market in the country. Thus, set in the Latin American actual situation, we undertake to understand the process and its consequences for Brazil, which, even with the third largest gas reserves in the region, in the short term, it follows as an importer of this feature. To have a concrete base with foundations and analyze what happens today, we must understand the political history of Bolivia and their internal changes. So we divided the work into two parts: first, we analyze the neo liberal period, and the agreements signed with Brazil in the hydrocarbon sector, for mutual benefits. In the second part, we understand the political rise of Evo Morales and the nationalization process of national wealth and the consequent crisis that developed with the international oil companies, especially with PETROBRAS, to the final outcome on the price, production and export of gas.

  8. The Bolivian natural gas crisis and the future of natural gas industry in Brazil; Crise do gas boliviano e o futuro da industria de gas natural no Brasil

    Energy Technology Data Exchange (ETDEWEB)

    Almeida, Edmar de

    2007-07-01

    The recent Bolivian natural crisis exhibits the worst scenery for the Brazilian natural gas industry. The government and PETROBRAS strategies for guaranteeing the gas supply undoubtedly failed. Independent of the discussion on the existence of other possible strategies, today the distributors and the consumers does not know how the PETROBRAS and government will guarantee the supply for the years to come. An also the agents do not know which will be the gas price in the future, as both the Bolivia but also the Natural Gas Industry in Brazil alternatives will be highly expensive.

  9. Mallas y flujos : acción colectiva, cambio social, quinua y desarrollo regional indígena en los Andes Bolivianos

    NARCIS (Netherlands)

    Laguna, P.

    2011-01-01

    This thesis studies collective action and social change in indigenous rural organisations (IRO) in the Bolivian Andes. I focus on the effects and importance that these organisations have in the historical process of regional development as social spaces that encapsulate different projects of social,

  10. Evidencias sedimentarias y geoquímicas de la pequena edad de hielo en el lago Milluni Grande del Altiplano boliviano

    Directory of Open Access Journals (Sweden)

    Matías Miguel Salvarredy-Aranguren

    2009-12-01

    Full Text Available El lago de Milluni Grande (LMG está ubicado en el flanco occidental de los Andes Orientales en el valle de Milluni que posee una clásica forma en U dado que se encuentra en una región glacial. El lago es el más grande de este valle, y dado que se halla situado al cierre de cuenca, resulta estratégico para albergar un registro sedimentario de los últimos 450 años. El registro sedimentario fue estudiado a partir de un testigo del lago, del cual se describieron y analizaron detalladamente parámetros físicos y químicos, sedimentología, mineralogía e indicadores geoquímicos los que permitieron evidenciar una importante variación del clima (VC. Adicionalmente, una datación con 210Pb en los niveles mas superficiales del testigo fue usada para establecer una tasa de sedimentación del lago y estimar una cronología para los niveles sin datación. La cronología definida señala que la variación del clima detectada sucede durante la Pequeña Edad de Hielo (LIA. Además, una correlación detallada fue establecida entre los niveles arenosos del registro sedimentario del LMG y las morrenas glaciarias del Cerro Charquini. Finalmente, algunas evidencias históricas bien documentadas sobre el desarrollo de Pequeña Edad de Hielo en Bolivia son mencionadas.

  11. PERSPECTIVES, NEGOTIATIONS AND DILEMMAS IN THE TRANSACTIONS BETWEEN YACIMIENTOS PETROLÍFEROS FISCALES BOLIVIANOS (YPFB AND YACIMIENTOS PETROLÍFEROS FISCALES (YPF, 1937-1945

    Directory of Open Access Journals (Sweden)

    María Cecilia Zuleta

    2013-01-01

    Full Text Available These research notes present a study about the linkages established between two state oil companies in South America, YPFB and YPF, in a few decades marked by economic, energy and political problems in Bolivia and Argentina. Founded in the image and likeness of YPF (Argentina entity created in 1922, state oil company vertically integrated, the YPFB (Bolivian entity created in 1936 developed from its origins several strategies to link in the technical, production, transportation and distribution of fuel, with the Argentinian YPF. The article provides specific information elements about connections, dealings, agreements and negotiations, dynamics, instruments and priorities, explaining its context and reasons. Moreover, this text explores the plot of negotiations between state oil companies, in order to clarify an earlier history of the most recent experiences of international interaction of state companies in the frame of energetical integration processes. It is proposed that the interaction between the two YPFs was ephemeral, because their agenda was overtaken by the favourable opportunities offered by cooperation within the framework of hemispheric programs, by agencies of the U.S. government.

  12. La “defensa social”. Pugnas y competencias en torno a la mano de obra en el Oriente Boliviano, 1890-1910.

    Directory of Open Access Journals (Sweden)

    Ana María Lema Garrett

    2010-01-01

    la región amazónica boliviana, al norte del país, suscitó profundos cambios en otros departamentos y, entre otras cuestiones, en el mercado laboral. Los departamentos de Beni y de Santa Cruz se constituyeron entonces en reservorios de mano de obra y el flujo migratorio hacia las regiones gomeras del norte fue creciendo proporcionalmente a las necesidades de los empresarios gomeros. Sin embargo, las regiones de origen, y específicamente Santa Cruz, reaccionaron en contra de este “vaciamiento”, desarrollando estrategias para controlar las migraciones o, eventualmente, frenarlas. Este proceso fue acompañado por el despliegue de varias normas novedosas en el ámbito laboral, producto de un debate parlamentario en torno al tema de las migraciones.

  13. PERSPECTIVES, NEGOTIATIONS AND DILEMMAS IN THE TRANSACTIONS BETWEEN YACIMIENTOS PETROLÍFEROS FISCALES BOLIVIANOS (YPFB) AND YACIMIENTOS PETROLÍFEROS FISCALES (YPF), 1937-1945

    OpenAIRE

    María Cecilia Zuleta

    2013-01-01

    These research notes present a study about the linkages established between two state oil companies in South America, YPFB and YPF, in a few decades marked by economic, energy and political problems in Bolivia and Argentina. Founded in the image and likeness of YPF (Argentina entity created in 1922), state oil company vertically integrated, the YPFB (Bolivian entity created in 1936) developed from its origins several strategies to link in the technical, production, transportation and distribu...

  14. PUMA-PUblication MAnagement: sistemi per la gestione delle pubblicazioni degli enti di ricerca

    OpenAIRE

    Biagioni, Stefania

    2005-01-01

    Lo scopo di questa presentazione ? quello di illustrare i) come ? stato realizzato un ciclo gestionale completo dell'informazione: dall'inserimento dei metadati e dei documenti digitali alla restituzione degli stessi all'utente finale; ii) come sono stati resi accessibili via web, a livelli diversi e secondo viste diverse l'informazione prodotta; iii) come siano stati creati servizi differenti utilizzando gli stessi oggetti. Vengono illustrati i sistemi utilizzati presso il CNR di Pisa per la...

  15. 2000 Census Public Use Microdata Area (PUMA) 5% for Hidalgo County, New Mexico, 2006se TIGER

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — The 2006 Second Edition TIGER/Line files are an extract of selected geographic and cartographic information from the Census TIGER database. The geographic coverage...

  16. 2000 Census Public Use Microdata Area (PUMA) 1% for Hidalgo County, New Mexico, 2006se TIGER

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — The 2006 Second Edition TIGER/Line files are an extract of selected geographic and cartographic information from the Census TIGER database. The geographic coverage...

  17. 2000 Census Public Use Microdata Area (PUMA) 1% for Grant County, New Mexico, 2006se TIGER

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — The 2006 Second Edition TIGER/Line files are an extract of selected geographic and cartographic information from the Census TIGER database. The geographic coverage...

  18. Three Axis Force Override Rate Control of a PUMA 560 Manipulator

    Science.gov (United States)

    1993-03-01

    and Intelligence, p. 242, McGraw-Hill, New York, 1987. 8. Whitney, D.E., "Historical Perspective and State of the Art in Robot Force Control ," Proceedings... Force Control ," Proceedings of the IEEE Conference on Robotics and Automation, (1987), pp.904-909. 12. Syvertsen, J. M., Force Override Rate...the IEEE Conference on Robotics and Automation, (1989), p. 102. 110 11. Eppinger, S.D., and Seering, W.P., "Understanding Bandwidth Limitations in Robot

  19. 2000 Census Public Use Microdata Area (PUMA) 1% for Union County, New Mexico, 2006se TIGER

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — The 2006 Second Edition TIGER/Line files are an extract of selected geographic and cartographic information from the Census TIGER database. The geographic coverage...

  20. 2000 Census Public Use Microdata Area (PUMA) 5% for Union County, New Mexico, 2006se TIGER

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — The 2006 Second Edition TIGER/Line files are an extract of selected geographic and cartographic information from the Census TIGER database. The geographic coverage...

  1. 2000 Census Public Use Microdata Area (PUMA) 1% for Harding County, New Mexico, 2006se TIGER

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — The 2006 Second Edition TIGER/Line files are an extract of selected geographic and cartographic information from the Census TIGER database. The geographic coverage...

  2. 2000 Census Public Use Microdata Area (PUMA) 5% for Socorro County, New Mexico, 2006se TIGER

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — The 2006 Second Edition TIGER/Line files are an extract of selected geographic and cartographic information from the Census TIGER database. The geographic coverage...

  3. 2000 Census Public Use Microdata Area (PUMA) 5% for Sierra County, New Mexico, 2006se TIGER

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — The 2006 Second Edition TIGER/Line files are an extract of selected geographic and cartographic information from the Census TIGER database. The geographic coverage...

  4. 2000 Census Public Use Microdata Area (PUMA) 5% for Lincoln County, New Mexico, 2006se TIGER

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — The 2006 Second Edition TIGER/Line files are an extract of selected geographic and cartographic information from the Census TIGER database. The geographic coverage...

  5. 2000 Census Public Use Microdata Area (PUMA) 5% for Quay County, New Mexico, 2006se TIGER

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — The 2006 Second Edition TIGER/Line files are an extract of selected geographic and cartographic information from the Census TIGER database. The geographic coverage...

  6. 2000 Census Public Use Microdata Area (PUMA) 5% for Harding County, New Mexico, 2006se TIGER

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — The 2006 Second Edition TIGER/Line files are an extract of selected geographic and cartographic information from the Census TIGER database. The geographic coverage...

  7. 2000 Census Public Use Microdata Area (PUMA) 5% for Torrance County, New Mexico, 2006se TIGER

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — The 2006 Second Edition TIGER/Line files are an extract of selected geographic and cartographic information from the Census TIGER database. The geographic coverage...

  8. 2000 Census Public Use Microdata Area (PUMA) 1% for Taos County, New Mexico, 2006se TIGER

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — The 2006 Second Edition TIGER/Line files are an extract of selected geographic and cartographic information from the Census TIGER database. The geographic coverage...

  9. 2000 Census Public Use Microdata Area (PUMA) 1% for Lincoln County, New Mexico, 2006se TIGER

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — The 2006 Second Edition TIGER/Line files are an extract of selected geographic and cartographic information from the Census TIGER database. The geographic coverage...

  10. 2000 Census Public Use Microdata Area (PUMA) 5% for Curry County, New Mexico, 2006se TIGER

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — The 2006 Second Edition TIGER/Line files are an extract of selected geographic and cartographic information from the Census TIGER database. The geographic coverage...

  11. 2000 Census Public Use Microdata Area (PUMA) 1% for Sierra County, New Mexico, 2006se TIGER

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — The 2006 Second Edition TIGER/Line files are an extract of selected geographic and cartographic information from the Census TIGER database. The geographic coverage...

  12. 2000 Census Public Use Microdata Area (PUMA) 1% for Torrance County, New Mexico, 2006se TIGER

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — The 2006 Second Edition TIGER/Line files are an extract of selected geographic and cartographic information from the Census TIGER database. The geographic coverage...

  13. 2000 Census Public Use Microdata Area (PUMA) 5% for Roosevelt County, New Mexico, 2006se TIGER

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — The 2006 Second Edition TIGER/Line files are an extract of selected geographic and cartographic information from the Census TIGER database. The geographic coverage...

  14. 2000 Census Public Use Microdata Area (PUMA) 1% for Mora County, New Mexico, 2006se TIGER

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — The 2006 Second Edition TIGER/Line files are an extract of selected geographic and cartographic information from the Census TIGER database. The geographic coverage...

  15. 2000 Census Public Use Microdata Area (PUMA) 5% for Guadalupe County, New Mexico, 2006se TIGER

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — The 2006 Second Edition TIGER/Line files are an extract of selected geographic and cartographic information from the Census TIGER database. The geographic coverage...

  16. 2000 Census Public Use Microdata Area (PUMA) 5% for Lea County, New Mexico, 2006se TIGER

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — The 2006 Second Edition TIGER/Line files are an extract of selected geographic and cartographic information from the Census TIGER database. The geographic coverage...

  17. 2000 Census Public Use Microdata Area (PUMA) 1% for Chaves County, New Mexico, 2006se TIGER

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — The 2006 Second Edition TIGER/Line files are an extract of selected geographic and cartographic information from the Census TIGER database. The geographic coverage...

  18. 2000 Census Public Use Microdata Area (PUMA) 1% for Quay County, New Mexico, 2006se TIGER

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — The 2006 Second Edition TIGER/Line files are an extract of selected geographic and cartographic information from the Census TIGER database. The geographic coverage...

  19. 2000 Census Public Use Microdata Area (PUMA) 5% for Grant County, New Mexico, 2006se TIGER

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — The 2006 Second Edition TIGER/Line files are an extract of selected geographic and cartographic information from the Census TIGER database. The geographic coverage...

  20. 2000 Census Public Use Microdata Area (PUMA) 1% for Otero County, New Mexico, 2006se TIGER

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — The 2006 Second Edition TIGER/Line files are an extract of selected geographic and cartographic information from the Census TIGER database. The geographic coverage...

  1. 2000 Census Public Use Microdata Area (PUMA) 5% for Cibola County, New Mexico, 2006se TIGER

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — The 2006 Second Edition TIGER/Line files are an extract of selected geographic and cartographic information from the Census TIGER database. The geographic coverage...

  2. 2000 Census Public Use Microdata Area (PUMA) 5% for Sandoval County, New Mexico, 2006se TIGER

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — The 2006 Second Edition TIGER/Line files are an extract of selected geographic and cartographic information from the Census TIGER database. The geographic coverage...

  3. 2000 Census Public Use Microdata Area (PUMA) 1% for Colfax County, New Mexico, 2006se TIGER

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — The 2006 Second Edition TIGER/Line files are an extract of selected geographic and cartographic information from the Census TIGER database. The geographic coverage...

  4. 2000 Census Public Use Microdata Area (PUMA) 1% for Catron County, New Mexico, 2006se TIGER

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — The 2006 Second Edition TIGER/Line files are an extract of selected geographic and cartographic information from the Census TIGER database. The geographic coverage...

  5. 2000 Census Public Use Microdata Area (PUMA) 5% for Bernalillo County, New Mexico, 2006se TIGER

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — The 2006 Second Edition TIGER/Line files are an extract of selected geographic and cartographic information from the Census TIGER database. The geographic coverage...

  6. 2000 Census Public Use Microdata Area (PUMA) 1% for Curry County, New Mexico, 2006se TIGER

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — The 2006 Second Edition TIGER/Line files are an extract of selected geographic and cartographic information from the Census TIGER database. The geographic coverage...

  7. 2000 Census Public Use Microdata Area (PUMA) 5% for Catron County, New Mexico, 2006se TIGER

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — The 2006 Second Edition TIGER/Line files are an extract of selected geographic and cartographic information from the Census TIGER database. The geographic coverage...

  8. 2000 Census Public Use Microdata Area (PUMA) 1% for Valencia County, New Mexico, 2006se TIGER

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — The 2006 Second Edition TIGER/Line files are an extract of selected geographic and cartographic information from the Census TIGER database. The geographic coverage...

  9. Zoonotic vector-borne bacterial pathogens in California mountain lions (Puma concolor), 1987-2010.

    Science.gov (United States)

    Girard, Yvette A; Swift, Pamela; Chomel, Bruno B; Kasten, Rickie W; Fleer, Katryna; Foley, Janet E; Torres, Steven G; Johnson, Christine K

    2012-11-01

    Sera collected from 442 mountain lions in 48 California counties between the years of 1987 and 2010 were tested using immunofluorescence assays and agglutination tests for the presence of antibodies reactive to Yersinia pestis, Francisella tularensis, Bartonella henselae, Borrelia burgdorferi, and Anaplasma phagocytophilum antigens. Data were analyzed for spatial and temporal trends in seropositivity. Seroprevalences for B. burgdorferi (19.9%) and B. henselae (37.1%) were relatively high, with the highest exposure in the Central Coast region for B. henselae. B. henselae DNA amplified in mountain lion samples was genetically similar to human-derived Houston-1 and domestic cat-derived U4 B. henselae strains at the gltA and ftsZ loci. The statewide seroprevalences of Y. pestis (1.4%), F. tularensis (1.4%), and A. phagocytophilum (5.9%), were comparatively low. Sera from Y. pestis- and F. tularensis-seropositive mountain lions were primarily collected in the Eastern and Western Sierra Nevada, and samples reactive to Y. pestis antigen were collected exclusively from adult females. Adult age (≥ 2 years) was a risk factor for B. burgdorferi exposure. Over 70% of tested animals were killed on depredation permits, and therefore were active near areas with livestock and human residential communities. Surveillance of mountain lions for these bacterial vector-borne and zoonotic agents may be informative to public health authorities, and the data are useful for detecting enzootic and peridomestic pathogen transmission patterns, particularly in combination with molecular characterization of the infecting organisms.

  10. 2000 Census Public Use Microdata Area (PUMA) 1% for Guadalupe County, New Mexico, 2006se TIGER

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — The 2006 Second Edition TIGER/Line files are an extract of selected geographic and cartographic information from the Census TIGER database. The geographic coverage...

  11. 2000 Census Public Use Microdata Area (PUMA) 1% for Bernalillo County, New Mexico, 2006se TIGER

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — The 2006 Second Edition TIGER/Line files are an extract of selected geographic and cartographic information from the Census TIGER database. The geographic coverage...

  12. 2000 Census Public Use Microdata Area (PUMA) 1% for Roosevelt County, New Mexico, 2006se TIGER

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — The 2006 Second Edition TIGER/Line files are an extract of selected geographic and cartographic information from the Census TIGER database. The geographic coverage...

  13. 2000 Census Public Use Microdata Area (PUMA) 1% for Lea County, New Mexico, 2006se TIGER

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — The 2006 Second Edition TIGER/Line files are an extract of selected geographic and cartographic information from the Census TIGER database. The geographic coverage...

  14. 2000 Census Public Use Microdata Area (PUMA) 1% for Socorro County, New Mexico, 2006se TIGER

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — The 2006 Second Edition TIGER/Line files are an extract of selected geographic and cartographic information from the Census TIGER database. The geographic coverage...

  15. 2000 Census Public Use Microdata Area (PUMA) 5% for Taos County, New Mexico, 2006se TIGER

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — The 2006 Second Edition TIGER/Line files are an extract of selected geographic and cartographic information from the Census TIGER database. The geographic coverage...

  16. 2000 Census Public Use Microdata Area (PUMA) 1% for Sandoval County, New Mexico, 2006se TIGER

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — The 2006 Second Edition TIGER/Line files are an extract of selected geographic and cartographic information from the Census TIGER database. The geographic coverage...

  17. 2000 Census Public Use Microdata Area (PUMA) 5% for Mora County, New Mexico, 2006se TIGER

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — The 2006 Second Edition TIGER/Line files are an extract of selected geographic and cartographic information from the Census TIGER database. The geographic coverage...

  18. 2000 Census Public Use Microdata Area (PUMA) 5% for Colfax County, New Mexico, 2006se TIGER

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — The 2006 Second Edition TIGER/Line files are an extract of selected geographic and cartographic information from the Census TIGER database. The geographic coverage...

  19. 2000 Census Public Use Microdata Area (PUMA) 5% for Eddy County, New Mexico, 2006se TIGER

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — The 2006 Second Edition TIGER/Line files are an extract of selected geographic and cartographic information from the Census TIGER database. The geographic coverage...

  20. Multiplex-detectie van Phytophthora: "padlock-based Universal Multiplex detection Array" (pUMA)

    NARCIS (Netherlands)

    Gaszczyk, K.; Mendes, O.; Verstappen, E.C.P.; Bonants, P.J.M.; Schoen, C.D.

    2010-01-01

    Plant Research International heeft een diagnostische methode ontwikkeld die toe te passen is 'in planta', en ook de meest recent beschreven (quarantaine-) soorten omvat. De methode omvat de ontwikkeling van een generieke Phytophthora-methode gevolgd door een Phytophthora-identificatie.

  1. Evaluating Landscape Connectivity for Puma concolor and Panthera onca Among Atlantic Forest Protected Areas

    Science.gov (United States)

    Castilho, Camila S.; Hackbart, Vivian C. S.; Pivello, Vânia R.; dos Santos, Rozely F.

    2015-06-01

    Strictly Protected Areas and riparian forests in Brazil are rarely large enough or connected enough to maintain viable populations of carnivores and animal movement over time, but these characteristics are fundamental for species conservation as they prevent the extinction of isolated animal populations. Therefore, the need to maintain connectivity for these species in human-dominated Atlantic landscapes is critical. In this study, we evaluated the landscape connectivity for large carnivores (cougar and jaguar) among the Strictly Protected Areas in the Atlantic Forest, evaluated the efficiency of the Mosaics of Protected Areas linked to land uses in promoting landscape connectivity, identified the critical habitat connections, and predicted the landscape connectivity status under the implementation of legislation for protecting riparian forests. The method was based on expert opinion translated into land use and land cover maps. The results show that the Protected Areas are still connected by a narrow band of landscape that is permeable to both species and that the Mosaics of Protected Areas increase the amount of protected area but fail to increase the connectivity between the forested mountain ranges (Serra do Mar and Serra da Mantiqueira). Riparian forests greatly increase connectivity, more than tripling the cougars' priority areas. We note that the selection of Brazilian protected areas still fails to create connectivity among the legally protected forest remnants. We recommend the immediate protection of the priority areas identified that would increase the structural landscape connectivity for these large carnivores, especially paths in the SE/NW direction between the two mountain ranges.

  2. 2000 Census Public Use Microdata Area (PUMA) 5% for Chaves County, New Mexico, 2006se TIGER

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — The 2006 Second Edition TIGER/Line files are an extract of selected geographic and cartographic information from the Census TIGER database. The geographic coverage...

  3. 2000 Census Public Use Microdata Area (PUMA) 1% for Eddy County, New Mexico, 2006se TIGER

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — The 2006 Second Edition TIGER/Line files are an extract of selected geographic and cartographic information from the Census TIGER database. The geographic coverage...

  4. 2000 Census Public Use Microdata Area (PUMA) 5% for Valencia County, New Mexico, 2006se TIGER

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — The 2006 Second Edition TIGER/Line files are an extract of selected geographic and cartographic information from the Census TIGER database. The geographic coverage...

  5. 2000 Census Public Use Microdata Area (PUMA) 1% for Cibola County, New Mexico, 2006se TIGER

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — The 2006 Second Edition TIGER/Line files are an extract of selected geographic and cartographic information from the Census TIGER database. The geographic coverage...

  6. 2000 Census Public Use Microdata Area (PUMA) 5% for Luna County, New Mexico, 2006se TIGER

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — The 2006 Second Edition TIGER/Line files are an extract of selected geographic and cartographic information from the Census TIGER database. The geographic coverage...

  7. 2000 Census Public Use Microdata Area (PUMA) 1% for Luna County, New Mexico, 2006se TIGER

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — The 2006 Second Edition TIGER/Line files are an extract of selected geographic and cartographic information from the Census TIGER database. The geographic coverage...

  8. 2000 Census Public Use Microdata Area (PUMA) 5% for Otero County, New Mexico, 2006se TIGER

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — The 2006 Second Edition TIGER/Line files are an extract of selected geographic and cartographic information from the Census TIGER database. The geographic coverage...

  9. Opinión de universitarios Bolivianos sobre el uso de alcohol en el contexto universitario Opinião de universitários Bolivianos sobre o uso de álcool no contexto universitário Bolivian university students' view of alcohol use in the university context

    Directory of Open Access Journals (Sweden)

    Nelly Balda-Cabello

    2011-06-01

    Full Text Available Se trata de una investigación cualitativa que enfocó el mundo universitario sobre los valores de vida utilizados por los jóvenes para lidiar con los peligros del consumo de alcohol. Fueron realizados grupos de discusión con 46 jóvenes universitarios. Los resultados evidenciaron que la sociedad boliviana es tolerante y permisiva en el consumo de alcohol de universitarios hombres. Los valores y el amor propio, el respeto por sí mismo, la responsabilidad y la capacidad de reflexión constituyeron un marco de actuación regulador, sin embargo no es generalizado.Esta é uma investigação qualitativa que enfocou o mundo universitário sobre os valores de vida, utilizados pelos jovens, para lidar com os perigos do consumo de álcool. Foram realizados grupos de discussão com 46 jovens universitários. Os resultados evidenciaram que a sociedade boliviana é tolerante e permissiva para o consumo de álcool de universitários homens. Os valores e o amor próprio, o respeito por si mesmo, a responsabilidade e a capacidade de reflexão constituíram marco de atuação regulador, porém, não é generalizado.Qualitative research inquiring into the university world about the values of young people's life to successfully offset the danger of alcohol consumption. Discussion groups were implemented in workshops with 46 university students. The results show that that Bolivian society is very tolerant and permissive towards alcohol consumption by male university students. Values like pride, self-respect, responsibility and capacity for reflection constitute a framework of regulatory action, but it is not generalized.

  10. “Los embrujos secretos del trópico”. Frontera amazónica y producción del espacio estatal en la obra cinematográfica de Jorge Ruiz

    Directory of Open Access Journals (Sweden)

    Roberto Pareja

    2014-11-01

    Full Text Available Este artículo estudia la obra cinematográfica de Jorge Ruiz utilizando la teoría socio-espacial de Henri Lefebvre. Argumenta que sus documentales, películas etnográficas y noticieros para el cine constituyen una totalidad discursiva que presenta estéticamente la producción del espacio estatal boliviano durante el siglo XX. A partir de una retórica audiovisual que juega con nociones de verticalidad, horizontalidad y centralidad, la obra de Ruiz posiciona el espacio andino como el eje de la modernización estatal y sitúa  a la cuenca amazónica en los márgenes del estado. Concluye que hacer visible la región amazónica en el discurso de la nación tuvo como efecto el desplazamiento de los antagonismos de clase en favor del conflicto entre centro y regiones.This article studies the cinematography of Jorge Ruiz making use of the socio-spatial theory of Henri Lefebvre. It argues that his documentaries, ethnographic films and newsreels for the cinema constitute a discursive totality that presents aesthetically the production of the Bolivian state space during the twentieth century. From an audiovisual rhetoric that plays with notions of verticality, horizontality and centrality, Ruiz’s work locates Andean space as the focal point of state modernization and situates the Amazon basin at the margins of the state. It concludes that in making the Amazon region visible in the nation’s discourse this had the effect of displacing class antagonisms in favor of the conflict between center and regions.

  11. Levaduras fermentadoras aisladas de Cyttaria hariotii (Fungi en bosques Andino-Patagónicos (Argentina Fermenting yeasts isolated from Cyttaria hariotii (Fungi in the Andean Patagonic Forest

    Directory of Open Access Journals (Sweden)

    José Ulloa

    2009-12-01

    Full Text Available Las levaduras han estado asociadas al hombre desde épocas muy tempranas. Entre estas se destacan las fermentadoras debido a su importancia en la industria de los alimentos. Las implicancias biotecnológicas de estas levaduras han sido extensamente estudiadas, sin embargo la distribución en la naturaleza y la ecología de estas no se encuentra igualmente documentada. El presente estudio se realizó en el Parque Nacional Nahuel Huapi (Noroeste Patagónico, Argentina sobre Cyttaria hariotii, hongo ascomicético parásito de árboles del género Nothofagus. Mediante el uso de un medio selectivo con etanol 8 % se obtuvieron 72 aislamientos. Los mismos fueron ordenados en cinco grupos en base a pruebas morfológicas y fisiológicas, tres de los cuales fueron asignados a los géneros Saccharomyces, Pichia y Kloeckera. El grupo Saccharomyces presentó el mayor número de aislamientos y se subdividió en tres subgrupos, dos de ellos presentan alta afinidad con S. bayanus y/ó S. uvarum. La totalidad de los aislamientos fueron psicrotolerantes y la temperatura máxima de crecimiento osciló entre 35 y 37 _C. El presente trabajo contribuye al conocimiento de la biodiversidad de levaduras de la Patagonia y representa el primer aislamiento masivo de levaduras sacaromicéticas en ambientes naturales de la región.Yeasts have been associated with mankind from early ages. Among these, fermentative yeasts played the most relevant role, due to its importance in the food industry. The biotechnological implications of these yeasts have been extensively studied. However there is a lack of information about its distribution in nature and ecology. In this study samples of Cyttaria hariotii, ascomycete fungus parasite of trees of the genus Nothofagus, were collected in Nahuel Huapi National Park (Northwestern Patagonia, Argentina. 72 isolates were obtained using a selective culture medium with 8% ethanol. Identification of the isolates was based on morphological and physiological tests, and then sorted into five groups. Three groups were assigned to the genera Saccharomyces, Pichia and Kloeckera respectively. The Saccharomyces group was the prevailing one, and was sorted into three subgroups. Two of them were related to S. bayanus and /or S. uvarum. All the isolates were psychrotolerant and the maximum growth temperature ranged between 35 and 37 _C. This work contributes to the knowledge of the biodiversity of yeasts in the region and represents the first massive isolation of Saccharomyces yeasts in natural environments of Patagonia.

  12. Evolución tectónica del Frente Andino en Neuquén Tectonicevolution of the Andean front in Neuquén

    Directory of Open Access Journals (Sweden)

    Gonzalo Zamora Valcarce

    2009-10-01

    Full Text Available La región preandina deNeuquén está conformada por una faja plegada fósil conocida como faja plegada ycorrida del Agrio. La excelente calidad de sus afloramientos, los datos desubsuelo, junto con recientes edades radimétricas y nuevos datos de trazas defisión en apatita, han permitido reconstruir con bastante precisión la historiaevolutiva de esta región. La estructuración de la región habría comenzadodurante el Cretácico Superior, documentado por la presencia de diquesbasálticos de rumbo E-O de ~100 Ma que se encuentran cortando la estructura dela zona interna. La deformación fue consecuencia de una leve horizontalizaciónde la placa subducida que habría provocada la migración del arco volcánicohacia el antepaís. Éste se habría instaurado en la región de Collipilli, endonde, recientemente se han obtenido edades de ~ 70 Ma para las FormacionesCollipilli y Cayanta. Estas unidades, se encuentran discordantes sobre lasecuencia mesozoica, marcando así el límite superior para el primer evento dedeformación. Evidencias de esta deformación en la zona externa de la fajaplegada se pueden observar en la discordancia del Grupo Neuquén sobre el GrupoBajada del Agrio (Rayoso. A su vez, los nuevos datos de trazas de fisión enapatita en el dorso de los Chihuidos, indican una serie de eventos deenfriamiento/levantamiento, el primero de ellos a los 70-50 Ma. El segundo enel Mioceno que produjo una reactivación de la deformación, evidenciada por losdepósitos sinorogénicos del Conglomerado Tralalhué y las Formaciones PuestoBurgos y Rincón Bayo. Los nuevos datos de trazas de fisión en apatita, permiteninterpretar otros dos eventos adicionales de levantamiento/enfriamiento entrelos 11 y los 6 Ma en el dorso de los Chihuidos.The Neuquén pre-Andean region is composed of a fossilfold belt known as the Agrio fold and thrust belt. The exceptional outcrops andthe subsurface data together with recent radiometric ages and new fission-trackages in apatite have allowed establishing the tectonic evolution of this area. Thedeformation began during the Late Cretaceous, documented by 100 Ma E-W trendingbasaltic dikes, cutting the cerro Mocho structure in the inner part of theAgrio fold and thrust belt. This event was a consequence of a gentle shallowingof the subducting plate that generated the eastward migration of the volcanicarc. This migration is documented in the Collipilli region, where recent radiometricages yielded ~ 70 Ma for the Collipilli and Cayanta Formations. They overlayunconformable the Mesozoic sequence, bounding the upper limit of the firstdeformational event. The unconformity between the Neuquén and Bajada del AgrioGroups in the outer zone was generated in this first event. The newfission-track data in the Los Chihuidos High indicate at least threecooling/uplift events, starting around 70-50 Ma. During the Miocene a newdeformational event occurred, as shown by the synorogenic deposits of Tralalhuéconglomerate, Puesto Burgos and Rincón Bayo Formations. The apatite fission-track data show other two cooling/uplift events between 11 and 6 Ma in the LosChihuidos High.

  13. Fragilidad del bosque de Fitzroya cupressoides (Mol. I M. Johnst. en un macizo andino patagónico chileno

    Directory of Open Access Journals (Sweden)

    Quintanilla Pérez, V.

    2005-12-01

    Full Text Available The study shows the difficult recuperation of the Fitzroya cupressoides (Mol l. M. Johnst. forest in a mountain volcanic chain located in the northern part of the patagonic region of Chili. The intense industrial exploitation of "alerce" initiated 80 years ago, the extended and long-term fire forest between 1950 to 1960, along with active mass removal processes in the andean mountain chain, generated huge impacts on the pluvial and alerce forests in that region, making difficult the recovery of this conifer wich is the most longlived in South America. Nevertheless, some case of regeneration of this species was observed, as it was declared in 1976 as natural monument in Chili.

    [fr] Nous avons étudié les difficultés de récupération d'une forêt de Fitzroya cupressoides (Mol. I. M. Johnst., dans une chaîne de montagnes volcaniques située au nord de la région patagonique chilienne. La forte exploitation de cette espèce a commencé il y a 80 ans, elle a été suivie d'incendies de long durée entre 1950 et 1960 et de très actifs processus de solifluxion dans les Andes. Ces phénomènes ont provoqué d'importants impacts sur les forêts humides et aussi sur les formations de ce conifère -la plante la plus longèvive de l'Amérique du Sud- dont la récupération actuelle se révèle très difficile. Cependant, malgré sa croissance très lente, nous avons observé quelques cas de régénération, en partie grâce à sa declaration au Chili comme monument naturel en 1976. [es] Se presenta un estudio referido a las dificultades de recuperación de un bosque de Fitzroya cupressoides (Mol I. M. Johnst. en una cadena montañosa volcánica localizada en el norte de la región patagónica de Chile. La intensa explotación industrial del alerce iniciada hace 80 años, los extensos y numerosos fuegos forestales de larga duración entre 1950 y 1960, junto con activos procesos de remoción en masa de la cordillera andina, provocaron importantes impactos en los bosques pluviales y en los de alerce de la región, dificultando la regeneración actual de esta conifera, que es la más longeva de Sudamérica. Sin embargo, a pesar de su crecimiento muy lento, se observan algunos casos de recuperación de esta especie, en parte debido a que fue declarada monumento natural en Chile durante el año 1976.

  14. Percepción de la participación de parteras y sanadores tradicionales andinos ecuatorianos en un programa de formación en salud

    Directory of Open Access Journals (Sweden)

    Verónica Inuca Tocagón

    2015-01-01

    Full Text Available El enlace entre la visión sanitaria occidental y la medicina indígena cimienta la identidad colectiva en la formación en salud. Analizar la percepción de la participación en un programa de formación en salud, desde la visión de parteras y sanadores tradicionales de la zona norte andina ecuatoriana. Diseño cualitativo de tipo interpretativo/ explicativo, se realizaron 25 entrevistas a profundidad y tres grupos focales a 20 parteras y 5 sanadores tradicionales, autodefinidos indígenas; el muestreo fue intencionado y razonado, de tipo opinático. Las subcategorías fueron definición de la participación en un programa de formación en salud, rol social y formación en salud y aplicación de la formación. El contraste de la narrativa se analizó a partir del sexo y tipología del informante. La participación en un programa de formación en salud fue descrita como la oportunidad para compartir, recuperar, conservar y fortalecer la medicina tradicional; opinar en cuestiones de salud-enfermedad en escenarios públicos como asambleas, y en escenarios privados como el acto de curar a la gente. La construcción de la participación en un programa de formación en salud, desde los actores de medicina tradicional enriquece el proceso.

  15. Cofradías de Arica y Tarapacá en los siglos XVIII y XIX. Indígenas andinos, sistema de cargos religiosos y festividades

    Directory of Open Access Journals (Sweden)

    Díaz A., Alberto

    2014-04-01

    Full Text Available Andean peoples expressed popular piety in several ways. Among them, the brotherhoods stand out as an institution inherited from colonial rule which was of great importance in the ritual congregation of the indigenous population, the construction of popular faith and the emergence of a ritual that incorporated elements of Roman Catholic liturgy, catechesis and local practices (ceremonies, dances, masks, costumes, musical instruments, melodies, etc.. This type of mutual aid religious institution, governed by a system of rotating positions, was comprised mainly of lay people who, on the basis of spirituality, the protection of a patron saint or Marian devotion, sought to promote charity and care for their members (brothers.Las poblaciones andinas expresaron de diversas formas la religiosidad popular. Entre ellas, las cofradías destacan por ser una institución heredada del régimen colonial que gravitó la congregación ritual de los indígenas, la construcción de una fe popular y el surgimiento de una ritualidad que integró los elementos de la liturgia catequética romana, así como prácticas locales (ceremonias, danzas, máscaras, vestimentas, instrumentos musicales, melodías, etc.. Este tipo de institución religiosa de socorro mutuo, regida por un sistema de cargos rotativos, estaba integrada preferentemente por laicos quienes sobre la base de la espiritualidad, la protección de un santo patrón o la advocación mariana, buscaban la promoción benéfica y asistencial para los cofrades (hermanos.

  16. Cargas subcorticales en el antepaís andino y la planicie pampeana: evidencias estratigráficas, topográficas y geofísicas

    Directory of Open Access Journals (Sweden)

    F.M. Dávila

    2005-12-01

    Full Text Available En la región sur de los Andes Centrales, la generación de espacios de acomodación en el antepaís ha sido explicada mediante la influencia de cargas supracorticales (apilamiento de escamas tectónicas y de cargas litostáticas. Estudios en la región de Sierras Pampeanas sugieren un patrón de subsidencia de longitud de onda regional (>500 km, difícilmente explicada por estas cargas generalmente asociadas a subsidencias de longitud de onda corta (<300 km. Se propone que los rasgos estratigráficoestructurales, topográficos y geofísicos en la región de Sierras Pampeanas y en la cuenca Chaco-paranaense pueden ser explicados mediante deflexiones inducidas por mecanismos subcorticales ocurridos en el manto astenosférico y/o la corteza inferior, como flujos astenosféricos termo-inducidos y/o eclogitización, respectivamente. Las cargas supracorticales se habrían superpuesto al fenómeno de subsidencia regional, solapando su influencia localmente.

  17. Cofradías de Arica y Tarapacá en los siglos XVIII y XIX. Indígenas andinos, sistema de cargos religiosos y festividades

    OpenAIRE

    Díaz A., Alberto; Martínez S., Paula; Ponce, Carolina

    2014-01-01

    Andean peoples expressed popular piety in several ways. Among them, the brotherhoods stand out as an institution inherited from colonial rule which was of great importance in the ritual congregation of the indigenous population, the construction of popular faith and the emergence of a ritual that incorporated elements of Roman Catholic liturgy, catechesis and local practices (ceremonies, dances, masks, costumes, musical instruments, melodies, etc.). This type of mutual aid religious instituti...

  18. Estudio de la bioacústica del zorzal andino (Turdus chiguanco con la aplicación del software Audacity, v. 2.0

    Directory of Open Access Journals (Sweden)

    Aldo Hugo Miguel Orellana

    2014-12-01

    Full Text Available Objetivos: Determinar las características bioacústicas del Turdus chiguanco; entre estas, la amplitud, la frecuencia y la duración en las distintas voces. Métodos: Investigación descriptiva en la cual se recolectaron datos bioacústicos durante los años 2011-2012, luego fueron procesados con el software Audacity versión 2.0. Resultados: Se identificaron cuatro tipos de voces: Voz de huida, voz estacionaria, voz de contacto y voz de canto. En la voz de huida, se determinaron valores promedio: amplitud máxima 52,82 dB; amplitud media 0,05 dB; amplitud mínima -13,91 dB; frecuencia máxima 6,8 kHz; frecuencia media 0,63 kHz; frecuencia mínima 0,27 kHz; la duración 1,05 segundos. En la voz estacionaria: amplitud máxima 31,22 dB; amplitud media -0,19 dB; amplitud mínima -16,45 dB; frecuencia máxima 4,31 kHz; frecuencia media 1,63 kHz; frecuencia mínima 0,52 kHz; duración de 0,29 segundos. En la voz de contacto: amplitud máxima 55,22 dB; amplitud media 0,8 dB; amplitud mínima -16,33 dB; frecuencia máxima 2,87 kHz; frecuencia media 0,41 kHz; frecuencia mínima 0,2 kHz; duración 1,28 segundos. Y en la voz de canto: amplitud máxima 50,22 dB; amplitud media 0,08 dB; amplitud mínima -7,38 dB; frecuencia máxima 3,85 kHz; frecuencia media 0,71 kHz; frecuencia mínima 0,33 kHz; duración 1,34 segundos. Conclusiones: El Turdus chiguanco emite cuatro tipos de voces: de huida, estacionaria, de contacto y de canto. Los valores de las variables bioacústicas: amplitud, frecuencia y duración, varían entre los cuatro tipos de voces.

  19. La huella ecológica y los países andinos, una reflexión sobre la sustentabilidad y la biocapacidad

    Directory of Open Access Journals (Sweden)

    Deyanira Gómez

    2013-09-01

    Full Text Available La escasez de los recursos ha llevado a la reflexión respecto a la medición del desarrollo, que ya no puede ser medido únicamente en términos de crecimiento económico como lo hace el Producto Interno Bruto (PIB, sino que es necesario tomar en cuenta los recursos o capital natural con los que cuenta una determinada nación para que desde una perspectiva de sustentabilidad, pueda asegurar la calidad de vida de las generaciones futuras, esta herramienta se la conoce como la Huella Ecológica.

  20. Ante los ojos del mundo: música, minería y conflicto social en el norte andino de Cajamarca, Perú

    Directory of Open Access Journals (Sweden)

    Marino Martínez

    2015-12-01

    Full Text Available Desde tiempos prehispánicos Cajamarca ha sido una importante zona agropecuaria. Pero en las décadas recientes se ha convertido en una zona de agudas convulsiones sociales. Durante el gobierno anticonstitucional de Alberto Fujimori (1990-2002, el estado permitió el ingreso de trasnacionales mineras para la extracción de oro a gran escala. Estas han dañado considerablemente el eco-sistema, la salud y el bienestar de la población local, creando descontento social y protestas. En ese contexto, el estado y la minería han impulsado una satanización de Cajamarca como un espacio cultural atrasado, reacio a las inversiones y al desarrollo. El presente artículo explora el rol que la música viene jugando en la representación de las protestas contra la minería y las actividades musicales y etnomusicológicas se complementan para contrarrestar una imagen demonizada por quienes defienden la inversión minera.

  1. Tectónica cuaternaria en el retroarco Andino a la latitud del volcán Tromen (37°s

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    Guido Gianni

    2014-12-01

    Full Text Available Estudios recientes focalizados en las vecindades del volcán Tromen, en el norte de la provincia de Neuquén, indican la presencia de estructuras afectando rocas de edad cuaternaria. En particular, un análisis detallado del flanco occidental del volcán Tromen muestra amplias zonas de deformación neotectónica, de las cuales sólo habían sido reconocidos sus sectores frontales. Se detallan geometría y mecanismos asociados a las principales estructuras cuaternarias a través de los dos valles principales que afectan este flanco volcánico. La coexistencia de estructuras neotectónicas contraccionales y distensivas es descripta como un rasgo distintivo. Así, el campo de esfuerzos asociado a este conjunto de estructuras es discutido: Mientras algunos estudios previos puntualizan que la deformación sería de carácter contraccional, otros apuntan a un régimen predominantemente distensivo. Finalmente, se plantea una alternativa que reconcilia ambos modelos en base a los nuevos datos.

  2. La transformación del Estado en América Latina: Una perspectiva económica desde los países andinos

    Directory of Open Access Journals (Sweden)

    Edgard Moncayo Jiménez

    2006-11-01

    Full Text Available A juicio de muchos analistas, el Estado-nación piedra angular de la organización política del mundo desde la suscripción del Tratado de Wesfalia, en 1968, está experimentando una profunda crisis. En esta visión, el poder efectivo de dicha entidad política se está desbordando en tres direcciones: hacia arriba, por la globalización y la supranacionalización; horizontalmente, por el desmonte del Estado de bienestar, la privatización y la desregulación; y hacia abajo, por la descentralización y la devolución de autonomías a las polis regionales y locales. La acción conjunta de estas tendencias estaría determinando el vaciamiento (

  3. HONGOS APHYLLOPHORALES (BASIDIOMYCOTA LIGNÍCOLAS DEL BOSQUE ALTO ANDINO “SANTA CLARA”. UNA GUÍA ILUSTRADA PARA EL ESTUDIO DE MACROHONGOS

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    César Augusto Pinzón Osorio

    2014-12-01

    Esta investigación surge de la necesidad de registrar los macrohongos Aphyllophorales de estos ecosistemas, conocidos por estar altamente impactados por la agricultura (Van der Hammen, 2003. Es fundamental el diseño de un material didáctico que ofrezca un panorama integral sobre la biología de los Aphyllophorales, teniendo en cuenta que las guías demacrohongos para el país, no se orientan directamente a su enseñanza

  4. Análisis filogenético de Paepalanthus (Eriocaulaceae Andinos seleccionados con énfasis en las especies presentes en Colombia

    Directory of Open Access Journals (Sweden)

    González Favio

    2006-06-01

    Full Text Available El género Paepalanthus Kunth, el más grande de la familia Eriocaulaceae, comprende aproximadamente 485 especies
    distribuidas en el neotrópico con unos pocos representantes en las regiones tropicales de África. En América, se encuentra desde México hasta Bolivia y Brasil, con una distribución altitudinal disyunta entre tierras bajas como los llanos de Colombia y Venezuela, los tepuyes de Venezuela y Guyana y la región amazónica de Colombia, Venezuela y Brasil, y regiones de alta montaña como los páramos de Costa Rica, Colombia, Venezuela, Ecuador y Perú. Estudios recientes con base en especies brasileñas y caracteres tanto morfológicos como anatómicos, sugieren que Paepalanthus es un grupo polifilético. El presente estudio se realizó con el propósito de integrar en un análisis filogenético tanto especies de zonas altas como de zonas bajas de Paepalanthus con el fin de evaluar la monofilia del grupo mediante un muestreo más riguroso, y de explorar las posibles relaciones biogeográficas de las especies de páramo. Los resultados confirman que Paepalanthus es un grupo polifilético, con P. saxicola en la
    base de las eriocaulaceae muestreadas, seguida por un grado conformado en su orden por especies de Philodice, Leiothrix y Blastocaulon. Enseguida, se encuentra el clado mayor formado por un subclado donde están (P. subtilis (P. barkleyi-P. lamarckii-P. tortilis. El segundo subclado presenta a P. fasciculatus en la base, seguida de dos subclados menores, uno de ellos conformado por especies de Paepalanthus, Tonina y Lachnocaulon, y el otro por especies de los géneros Actinocephalus, Leiothrix, Syngonanthus, Eriocaulon y la mayoría de las especies de Paepalanthus propias de los páramos. De esta forma, el subgénero Psilandra representado por la especie P. saxicola se propone como el más basal y el subgénero Platycaulon, que resulta monofilético con el muestreo disponible, es el grupo más anidado dentro de la filogenia de Paepalanthus. La serie leptocephali s.l. del subgénero Paepalocephalus resulta polifilética. El análisis filogenético sugiere que un grupo de especies presentes en los páramos de Colombia, Venezuela y Perú, pudo haber compartido una ancestría en común con especies de Norteamérica; y que otro grupo compartió ancestría con especies de zonas bajas de Colombia, Venezuela, Guyana y Brasil.

  5. Experiencia de investigación acción participativa en cultivos andinos con autoinsumos agroecológicos : Comunidad rural de Coctaca, Humahuaca, Jujuy

    OpenAIRE

    Gailán, D.; Tactaca, E.; Tactaca, P.; Álvarez, Susana Edith; Geronazzo, A.; Alvarracin, Adriana

    2015-01-01

    El objetivo de la experiencia fue evaluar un biol a base se lombricompuesto sobre parámetros de crecimiento y rendimiento de maíz y papa andina, con productores del paraje de Coctaca. En maíz se utilizó Te de lombricompuesto (BFL) y en papa (BFL), y BFL combinado con una cepa local deTrichoderma spp. La evaluación se realizó al momento de la cosecha en la misma parcela. En maíz de midió altura de planta, número de mazorcas/planta y peso de mazorcas; en papa: número de tubérculos/planta, peso ...

  6. EFECTOS DE LA ILUMINACIÓN RELATIVA Y LA FERTILIZACIÓN SOBRE EL CRECIMIENTO DE PLÁNTULAS DE ROBLE ANDINO EN VIVERO

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    YIRA LUCIA SEPÚLVEDA

    2014-01-01

    Full Text Available Quercus humboldtii es una especie vegetal nativa de mucha importancia en Colombia por su uso en repoblamiento forestal y restauración de tierras altoandinas degradadas. La especie se encuentra fuertemente amenazada y es necesario establecer programas de propagación de la misma. Sin embargo, poco se conoce sobre sus exigencias nutricionales y lumínicas. El objetivo de este estudio fue determinar los efectos simples y combinados de la iluminación relativa (IR y la fertilización sobre el crecimiento de plántulas de Q. humboldtii en vivero. Para esto se establecieron en combinación tres condiciones contrastantes de iluminación relativa (alta, media y baja IR y nueve tratamientos de fertilización: completa (TC, con un elemento faltante (-N, -P, -K, -Ca, -Mg, -S, -B y un testigo sin fertilización (T0. La condición de IR media presentó el mejor desarrollo de las plántulas. Todos los tratamientos con deficiencias nutricionales produjeron disminuciones en el desarrollo de las plántulas con respecto a TC, excepto en el tratamiento -B. El N fue el elemento más limitante, con rendimientos similares a los del tratamiento testigo. El orden de limitación que produjeron los tratamientos aplicados siguió la secuencia: T0,-N > -Ca, -K, -P > -Mg, -S > TC,-B. No se detectó interacción significativa IR x Fertilización sobre el desarrollo de las plántulas.

  7. Caracterización de microorganismos involucrados en la descomposición de material vegetal consumido por Oso Andino Tremarctos ornatus (Ursidae

    Directory of Open Access Journals (Sweden)

    Pinzón María Alejandra

    2005-12-01

    Full Text Available Se establecieron bases para un método de datación de hojas consumidas por Tremarctos ornatus al establecer presencia o ausencia de microorganismos en restos vegetales de Tillandsia spp., recogidos en campo y que corresponden a diferentes etapas de descomposición. Las muestras (2 cm a la base de la hoja consumida por el animal, fueron posteriormente procesadas en el laboratorio y se realizó la caracterización y determinación de los microorganismos presentes. Los morfotipos bacterianos predominantes fueron los cocobacilos y bacilos Gram (-, los primeros se encontraron en todas las edades de descomposición y los segundos solo en las muestras
    menores a seis meses. Cocobacilos Gram (+ solo aparecieron en hojas entre uno y tres meses y los bacilos Gram (+ exclusivamente en hojas de menos de un mes. Cocos y diplococos se presentaron únicamente en la muestra de hoja de cinco y seis meses en descomposición. Se encontraron cuatro morfotipos de hongos, de los cuales se identificaron Mucor spp. y Thricoderma spp. Los otros dos morfotipos no fueron identificados. Thricoderma spp. se encuentra asociado a por lo menos un hongo más.

  8. Conflicto colonial andino y mediación teológica en la crónica de Guamán Poma

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    Fernando Amaya Farías

    2012-06-01

    Full Text Available La Nueva corónica y buen gobierno de Felipe Guamán Poma de Ayala representa un documento de protesta muy singular en palabras e imágenes, como defensa de los indígenas maltratados y explotados por el sistema colonial. El cronista como indígena cristiano critica a los funcionarios políticos y religiosos de la colonia, posición que no implica necesariamente la ruptura con el sistema colonial. Para Guamán Poma, lo que amenaza la vida de las culturas indígenas no es la predicación de la salvación ni la forma ideal de vida cristiana, sino las concreciones históricas con las que el cristianismo es transmitido en la Colonia. La mediación teológica que plantea el cronista la formula desde la pregunta por la ruptura y continuidad, por la compatibilidad e incompatibilidad que se presenta entre las religiones andinas y el cristianismo.La nueva corónica y buen gobierno by Felipe Guamán Poma de Ayala represents a document of protest outstanding in words and images for the defense of the oppressed natives. The writer’s own experience as native and Christian is the startingpoint for his citizism of the colonial system. In his opinion the negative influence on the native culture wasn’t caused by the Christian promise of salvation nor by the proclaimed ideal way of Christian living, but by the way Christianity was transformed through the colonial context. The author´s main interest is to raise the question of the interruption and continuance and the compatibility and incompatibility between the native religions and Christianity.

  9. Community-Based Tourism and Peasant Differentiation: Considerations from an Andean case Turismo rural comunitario y diferenciación campesina: Consideraciones a partir de un caso andino

    Directory of Open Access Journals (Sweden)

    Jordi Gascón

    2011-01-01

    Full Text Available In the last years, Community-Based Tourism has created expectations in many latin american rural communities as an strategy to increase their income and to diversify the sources of this income. But it is an activity that is not free of risks. From a specific case (Amantaní Island, Titikaka Lake, Peru, the article studies one of these risks: the impact of tourism in the socio-economical communitarian cohesion.En los últimos años, el turismo rural comunitario se ha presentado como un instrumento adecuado para aumentar la renta de la población campesina y diversificar sus fuentes de ingresos. Pero se trata de una actividad económica que no está exenta de riesgos. A partir de un caso específico (la isla peruana de Amantaní, en el Lago Titicaca el artículo estudia uno de ellos: el impacto del turismo en la cohesión socioeconómica comunitaria.

  10. Caracterización de la biodiversidad acuática en la cuenca andino-amazónica de Madre de Dios - Perú

    OpenAIRE

    2016-01-01

    El conocimiento de los cuerpos de agua de la región Neotrópical se revela como una necesidad a nivel global que la sociedad debe afrontar. La biodiversidad acuática, especialmente las comunidades de peces de la Amazonía, la principal cuenca del Neotrópico, constituyen el principal reservorio de diversidad de agua continental del mundo, señalada como una región de alta prioridad para la conservación. No obstante, aun presenta muchas lagunas de conocimiento con el agravante de estar registrando...

  11. «Sangres» para propiciar la vida: metáforas y creencias sobre la concepción «humana» en el altiplano andino

    Directory of Open Access Journals (Sweden)

    Vicente Martín, Patricia

    2016-06-01

    Full Text Available This article reflects on the participation of telluric entities in regulating and propitiating fertility in general and «human» fertility in particular in the Andes region. The first part of this study deals with the participation of telluric forces in the breeding of livestock and the propitiation of plant life, as well as resources in general. In view of the visibility of these propitiatory rites, the genesis of new individuals is couched in a language in which references to «blood» and excessive heat abound. This study of the notions relating to conception and human fertility in the Andes involves the systematization of references found in ethnographic literature, and presentation of my own research in rural areas of southern Potosi (Bolivia. I then go on to consider the bodily rhetoric of life-promoting pregnant bodies and their specificities with a view to probing more deeply into the concept of fertility of the people of the Andean plateau.Este artículo tiene como objetivo la reflexión sobre la participación de las entidades tutelares en la regulación y propiciación de la fertilidad en general y de la «humana» en particular en los Andes. La primera parte del estudio aborda la participación de las fuerzas telúricas en la multiplicación del ganado y la propiciación de la vida vegetal, así como de las riquezas en general. Frente a la visibilidad de estos ritos propiciatorios, la génesis de los nuevos individuos se enmascara en un lenguaje en el cual abundan las referencias a las «sangres» y al exceso de calor. El estudio de las ideas acerca de la concepción y la fertilidad humana en los Andes pasa por una sistematización de las referencias halladas en la literatura etnográfica, y por la exposición de mis propias investigaciones en áreas rurales de Potosí. Tras ello se abordan las retóricas corporales de los cuerpos gestantes que propician la vida y sus especificidades con el fin de ahondar en la compresión de los conceptos de fertilidad de las gentes del altiplano.

  12. PRODUCCIÓN DE HOJARASCA FINA EN BOSQUES ALTO ANDINOS DE ANTIOQUIA, COLOMBIA FINE LITTER PRODUCTION IN HIGH ANDEAN FORESTS FROM ANTIOQUIA, COLOMBIA

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    Claudia Marcela Zapata Duque

    2007-06-01

    Full Text Available En bosques montanos naturales de Quercus humboldtii y reforestados (Pinus patula y Cupressus lusitanica de la región de Piedras Blancas, Antioquia (Colombia, fue evaluada la producción de hojarasca por un periodo de 2 años. Se utilizaron trampas de hojarasca con el fin de recoger el material desprendido del dosel para su posterior separación en fracciones y pesado respectivo. El promedio de caída de hojarasca anual para Q. humboldtii, P. patula y C. lusitanica fue de 7877,20; 8362,47 y 3725,97 kg ha-1año-1 respectivamente; siendo la fracción foliar la que mayor participación tuvo en la producción total. Mediante análisis de regresión lineal múltiple se ajustaron modelos de producción de hojarasca según fracciones por cobertura en función de diferentes variables hidrológicas, tales como la intensidad y la cantidad de lluvia del período simultáneo a la recolección de la hojarasca o inmediatamente anterior.Litter production was measured over two years in a montane oak forest (Quercus humboldtii and in pine (Pinus patula and cypress (Cupressus lusitanica plantations in Piedras Blancas, Antioquia ( Colombia . Litter traps were used in order to collect litterfall to be subsequently separated into fractions and weighed. Annual mean litterfall for Q. humboldtii, P. patula and C. lusitanica was of 7877,20; 8362,47 and 3725,97 kg ha-1year-1 respectively; being the leaf fraction of highest participation in total production. Multiple linear regression models were used to fit litter production for each fraction and forest cover as a function of different hydrological variables such as intensity and quantity of precipitation, both during the period when the leaf litter was collected and immediately preceding one.

  13. Narrativas del paisaje andino colombiano: visión ecológica en la música carranguera de Jorge Velosa

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    Felipe Cárdenas

    2009-05-01

    Full Text Available Este es el resultado de un estudio que indagó en las visiones políticas plasmadas en el discurso ambiental que expresa la música Carranguera en la obra de Jorge Velosa. El material de investigación parte del análisis de las trece canciones que conforman el compendio titulado En Cantos Verdes (1998. El trabajo se planteó como una investigación de tipo estructural, que apeló a nociones de la hermenéutica, la semiótica y al análisis del discurso. La realidad de la vida campesina se estructura en un rico universo cultural que la música y el canto refleja a modo de meta-discurso de las sociedades campesinas andinas de Colombia.

  14. Model of automatic fuel management for the Atucha II nuclear central with the PUMA IV code; Modelo de gestion automatica de combustible para la Central Nuclear Atucha II con el codigo PUMA IV

    Energy Technology Data Exchange (ETDEWEB)

    Marconi G, J.F.; Tarazaga, A.E.; Romero, L.D. [CNEA, Av. Gral. Paz 1499 San Martin-Bs. As. (Argentina)]. e-mail: marconi@cnea.gov.ar

    2007-07-01

    The Atucha II central is a heavy water power station and natural uranium. For this reason and due to the first floor reactivity excess that have this type of reactors, it is necessary to carry out a continuous fuel management and with the central in power (for the case of Atucha II every 0.7 days approximately). To maintain in operation these centrals and to achieve a good fuels economy, different types of negotiate of fuels that include areas and roads where the fuels displace inside the core are proved; it is necessary to prove the great majority of these managements in long periods in order to corroborate the behavior of the power station and the burnt of extraction of the fuel elements. To carry out this work it is of great help that a program implements the approaches to continue in each replacement, using the roads and areas of each administration type to prove, and this way to obtain as results the one regulations execution in the time and the average burnt of extraction of the fuel elements, being fundamental this last data for the operator company of the power station. To carry out the previous work it is necessary that a physicist with experience in fuel management proves each one of the possible managements, even those that quickly can be discarded if its don't fulfill with the regulatory standards or its possess an average extraction burnt too much low. For this it is of fundamental help that with an automatic model the different administrations are proven and lastly the physicist analyzes the more important cases. The pattern in question not only allows to program different types of roads and areas of fuel management, but rather it also foresees the possibility to disable some of the approaches. (Author)

  15. The new 1.7 ltr. Zetec-SE engine for Ford`s sports coupe Puma; Der neue 1,7-l-Zetec-SE-Motor fuer das Sportcoupe Puma

    Energy Technology Data Exchange (ETDEWEB)

    Heuser, G. [Ford Werke AG, Koeln (Germany); Oppel, R. [Ford Werke AG, Koeln (Germany); Eden, M.; Farenden, G.; Warren, G.A. [Ford Werke AG, Koeln (Germany); Menne, R.J. [Ford Werke AG, Koeln (Germany)

    1997-04-01

    For the 1996 model year, as well as a much revised Fiesta, Ford has introduced an entire generation of newly-developed engines. These were four-valve engines with swept volumes of 1.25 and 1.4 litres. On the basis of these engines, in close collaboration with Yamaha Ford has developed a high-performance engine with a swept volume of 1.7 litres. This engine will be launched in the course of this year in the sports coupe based on the Fiesta. In this engine, Ford is using a continuously variable advance/retard adjustment of the inlet camshaft for the first time. The essential features of this new engine are presented in this article, focusing on the most important differences and innovations compared with the 1.25 ltr. and 1.4 ltr. engines. (orig.) [Deutsch] Mit dem Modelljahr 1996 fuehrte Ford zusammen mit dem stark ueberarbeiteten Fiesta eine komplett neu entwickelte Motorengeneration ein. Hierbei handelt es sich um Motoren in Vierventiltechnik mit Hubraeumen von 1,25 l und 1,4 l. Basierend auf diesen Motoren hat Ford in enger Zusammenarbeit mit Yamaha einen Hochleistungsmotor mit einem Hubraum von 1,7 l entwickelt. Er kommt im Verlauf dieses Jahres im auf dem Fiesta basierenden Sportcoupe zum Einsatz. Bei diesem Motor setzt Ford erstmals eine kontinuierlich einstellbare Einlassnockenwellen-Verstellung ein. In diesem Beitrag wird der neue Motor in seinen Grundzuegen vorgestellt. Den Schwerpunkt stellt jedoch die Darstellung der wichtigsten Unterschiede und Neuerungen im Vergleich zum 1,25-l- und zum 1,4-l-Motor dar. (orig.)

  16. Gabriela Ramos, Muerte y conversión en los Andes. Lima y Cuzco, 1532-1670, Lima, Instituto de Estudios Peruanos (IEP), Instituto Francés de Estudios Andinos (IFEA), Cooperación Regional para los Países Andinos, 2010, 362 p.

    OpenAIRE

    Argouse, Aude

    2011-01-01

    El libro que presentamos se inscribe en el ámbito de los estudios importantes, aparecidos desde los años noventa, sobre los cambios introducidos en las poblaciones autóctonas del virreinato del Perú con la llegada del cristianismo. « Más allá de la resistencia », tal como se titulaba un libro de contribuciones publicado en los años 2 000, los estudios sobre los habitantes de los Andes se dedican a buscar en qué medida y cómo los indígenas participaron también a su « transformación religiosa »...

  17. ¿Cómo hacer chicha en Córdoba? Reflexiones en torno a los aspectos políticos y materiales de la producción de chicha por inmigrantes bolivianos

    Directory of Open Access Journals (Sweden)

    Francisco Pazzarelli

    2009-04-01

    Full Text Available ResumenLas bebidas y las comidas pueden ser consideradas como instancias con una cotidianeidad particular,y con una poética y una gastro-política específicas, que se involucran en la reproducción cotidiana yen los sentidos de pertenencia de un grupo. En esas instancias convergen distintos sujetos ymaterialidades que se articulan en un proceso en el que ellos mismos son definidos. Desde estaperspectiva, nos interesa reflexionar en torno de los procesos de producción de chicha (bebidafermentada a base de maíz por parte de mujeres inmigrantes bolivianas y su articulación con otroscontextos en la ciudad de Córdoba (Argentina. Consideramos que existe una tensión entre la necesidadde contar con esta bebida para ciertas celebraciones y la dificultad que existe para producirla, y quedicha tensión se inscribe en las situaciones de marginalidad que experimentan la mayoría de losinmigrantes. Para aproximarnos a la gastro-política que se despliega en estos procesos de producción,nos concentraremos en las trayectorias de las mujeres chicheras, así como de las materias primas yobjetos necesarios para su elaboración. AbstractBeverages and food might be considered as instances with a special daily routine, and with a poeticand a special gastro-politic, which implicates in the everyday reproduction and in the sense ofmembership in a group. In these instances different subjects and materialities convergence, whicharticulates in a process in which themselves are defined. From this perspective, it interests us toreflect around the «chicha» (a fermented beverage based on maize production processes from the viewof the Bolivian women immigrants and it articulation with other contexts in the city of Córdoba(Argentina. We consider that it exists a tension between the necessity to count with this beverage forcertain celebrations and the difficulty to produce it, and the tension which are inscribed in the marginalsituations that experience the majority of the immigrants. To approximate the gastro-politics thatunfolds in these production processes, we concentrate on the trajectories of the «chichera» women, asthe source materials and other necessities for its elaboration.

  18. Inserción de horticultores bolivianos en Río Cuarto (Argentina Procesos de inmigración, trabajo y conformación de economías étnicas

    Directory of Open Access Journals (Sweden)

    Roberto Rodolfo Benencia

    2016-12-01

    En este artículo trataremos de apreciar si estos procesos migratorio-laborales en los que se produce una concentración de inmigrantes de una misma nacionalidad en algunas áreas económico-laborales de la Argentina, como por ejemplo en la horticultura de Río Cuarto (provincia de Córdoba, y en la que tanto los patrones como los empleados pertenecen a la misma nacionalidad migrante, pueden ser considerados economías o negocios étnicos, como han sido catalogados en otros países por la literatura especializada.

  19. Del altiplano sur boliviano hasta el mercado global: coordinación y estructuras de gobernancia en la cadena de valor de la quinua orgánica y del comercio justo

    NARCIS (Netherlands)

    Laguna, P.; Cáceres, Z.; Carimetrand, A.

    2006-01-01

    This article aims to describe and to analyze the recent development of real organic quinoa global value chain, linked to consumer demand in specific quality food markets. Taking into account small producers¿ organizations and local and multinational private companies, the authors consider the evolut

  20. La frontière boliviano-paraguayenne : des contentieux historiques aux dynamiques d’intégration énergétiques (The Bolivia-Paraguay Border: From Old Enemies to the Integrated Energy Powerhouses

    Directory of Open Access Journals (Sweden)

    Laurent Lacroix

    2011-01-01

    Full Text Available En avril 2009, la Bolivie et le Paraguay signent un traité de démarcation frontalière qui met fin à un contentieux frontalier né de la guerre du Chaco (1932-1935. Pour les deux seuls pays enclavés du continent américain, l'enjeu consiste désormais à réhabiliter une région frontalière qui a longtemps constitué un glacis. Malgré les projets d'intégration économique et de coopération bilatérale, la frontière reste un thème sensible, à l'origine de quelques tensions diplomatiques et militaires. Les deux pays devront outrepasser ces contretemps liés à la mémoire collective encore marquée par le conflit armé qui les a opposés. Sans quoi, le Chaco pourrait bien demeurer un angle mort dans les mégaprojets définis dans le cadre de l'intégration de la région sud-américaine (IIRSA et rester un espace idéal pour les contrebandiers et les trafiquants.(In April 2009, Bolivia and Paraguay signed a treaty establishing their border, ending a dispute born of the war of Chao (1932-1935. For the two land-locked countries of the American continent the challenge was to establish a border region which had long been a buffer zone. Despite economic integration and bilateral cooperation, the border remains a sensitive issue and has been the source of diplomatic and military tension. The two countries must surpass these difficulties stemming from the collective memory of the armed conflict between them. Otherwise Chao will remain the stumbling block to the megaprojects of South American regional integration (IIRSA and borders will remain an ideal place for smugglers and drug traffickers.

  1. El comportamiento de las tasas de interés en el sistema bancario boliviano y el margen del Banco Central de Bolivia para políticas de tasas de interés

    OpenAIRE

    Marco Antonio Laguna Vargas

    1999-01-01

    Se estudian las posibilidades del BCB para influir en el comportamiento de las tasas de interés del sistema bancario, en una estructura de mercado del sistema financiero no competitiva. Adicionalmente, se emplea un modelo de extracción de señales para analizar la respuesta de los bancos a la variabilidad de la política de operaciones de mercado abierto. El comportamiento de las tasas de interés que predicen estos modelos puede explicar los resultados de trabajos anteriores que señalan una res...

  2. Análisis de las transformaciones de la estructura agraria hortícola platense en los últimos 20 años : El rol de los horticultores bolivianos

    OpenAIRE

    García, Matías

    2012-01-01

    La presente tesis en su conjunto provee un análisis de la producción, la tecnología, la tierra, los agentes de la producción, la comercialización, la fuerza de trabajo que, en interacción, brindan elementos no sólo para interpretar las transformaciones en la estructura agraria hortícola platense en los últimos 20 años, sino que también logran desarrollar o poner en cuestión el nuevo modelo de producción y abastecimiento a nivel agregado. Estas transformaciones fueron descriptas y analizada...

  3. Análisis de las transformaciones de la estructura agraria hortícola platense en los últimos 20 años : El rol de los horticultores bolivianos

    OpenAIRE

    García, Matías

    2012-01-01

    La presente tesis en su conjunto provee un análisis de la producción, la tecnología, la tierra, los agentes de la producción, la comercialización, la fuerza de trabajo que, en interacción, brindan elementos no sólo para interpretar las transformaciones en la estructura agraria hortícola platense en los últimos 20 años, sino que también logran desarrollar o poner en cuestión el nuevo modelo de producción y abastecimiento a nivel agregado. Estas transformaciones fueron descriptas y an...

  4. ¿Cómo hacer chicha en Córdoba? Reflexiones en torno a los aspectos políticos y materiales de la producción de chicha por inmigrantes bolivianos

    Directory of Open Access Journals (Sweden)

    Gisela Vargas Ibarra

    2008-09-01

    Full Text Available Beverages and food might be considered as instances with a special daily routine, and with a poeticand a special gastro-politic, which implicates in the everyday reproduction and in the sense ofmembership in a group. In these instances different subjects and materialities convergence, whicharticulates in a process in which themselves are defined. From this perspective, it interests us toreflect around the «chicha» (a fermented beverage based on maize production processes from the viewof the Bolivian women immigrants and it articulation with other contexts in the city of Córdoba(Argentina. We consider that it exists a tension between the necessity to count with this beverage forcertain celebrations and the difficulty to produce it, and the tension which are inscribed in the marginalsituations that experience the majority of the immigrants. To approximate the gastro-politics thatunfolds in these production processes, we concentrate on the trajectories of the «chichera» women, asthe source materials and other necessities for its elaboration

  5. Construcción de la noción de estado en función de la voz de otro: el discurso del vicepresidente boliviano Álvaro García Linera

    Directory of Open Access Journals (Sweden)

    Fernández, María del Rosario

    2011-01-01

    Full Text Available [es] En este artículo, analizamos la conferencia pronunciada por el Vicepresidente de Bolivia, Álvaro García Linera, en la Facultad de Derecho de la Universidad de Buenos Aires, el 8 de abril de 2010. El trabajo se encuadra en una investigación acerca del discurso político y la construcción de la noción de estado a partir de la promulgación de la nueva Construcción del Estado (2007. Primero, situaremos, en el contexto de la escena englobadora del discurso académico, a la conferencia como escena genérica e indicaremos sus diversas escenografías. Ello, considerando que el género discursivo resulta el primer gran otro en función del cual se construyen los discursos. Nuestra hipótesis interpretativa sostiene que el discurso acerca del estado se vincularía con la contraargumentación (implícita o explícita del otro y ello se plasmaría en diversas estrategias lingüísticas: las negaciones polémicas y metalingüísticas; el uso de conectores contraargumentativos o antiorientados; la presencia de ciertas formas deícticas. Estos dos últimos aspectos se irán señalando en los diversos análisis. Nuestro aporte radica, creemos, en proveer una herramienta interpretativa a partir del análisis del discurso que favorezca una lectura interdisciplinaria en las áreas de la Ciencia Política y la Comunicación. [en] In this paper, we analyze the speech delivered by Vice-President of Bolivia, Alvaro Garcia Linera, at the Law Faculty at the University of Buenos Aires on April 8, 2010. The work is part of a research about the political discourse and the construction of the notion of state since the enactment of the new State Building (2007. First, the conference will be placed, in the context of the scene encompassing the academic discourse, as a generic scene and the different scenery will be indicated. This, considering that the discourse genre results in the first major other in terms of which discourses are constructed. Our interpretative hypothesis argues that the discourse about the state would be associated with the counter-argument (implicit or explicit of the other and this would be reflected in different linguistic strategies: the controversial and metalinguistic negations; the use of counter-argumentative connectors; the presence of certain deictic forms. The latter two will be pointed out in the different analysis. Our contribution lies, we believe, in providing an interpretative tool coming from discourse analysis that would promote an interdisciplinary reading in the areas of Political Science and Communication

  6. Exacerbations and health care resource utilization in patients with airflow limitation diseases attending a primary care setting: the PUMA study

    Science.gov (United States)

    Montes de Oca, Maria; Aguirre, Carlos; Lopez Varela, Maria Victorina; Laucho-Contreras, Maria E; Casas, Alejandro; Surmont, Filip

    2016-01-01

    Background COPD, asthma, and asthma–COPD overlap increase health care resource consumption, predominantly because of hospitalization for exacerbations and also increased visits to general practitioners (GPs) or specialists. Little information is available regarding this in the primary care setting. Objectives To describe the prevalence and number of GP and specialist visits for any cause or due to exacerbations in patients with COPD, asthma, and asthma–COPD overlap. Methods COPD was defined as post-bronchodilator forced expiratory volume in 1 second/forced vital capacity (FEV1/FVC) ratio <0.70; asthma was defined as prior medical diagnosis, wheezing in the last 12 months, or wheezing plus reversibility (post-bronchodilator FEV1 or FVC increase ≥200 mL and ≥12%); asthma–COPD overlap was defined as post-bronchodilator FEV1/FVC <0.70 plus prior asthma diagnosis. Health care utilization was evaluated as GP and/or specialist visits in the previous year. Results Among the 1,743 individuals who completed the questionnaire, 1,540 performed acceptable spirometry. COPD patients had a higher prevalence of any medical visits to any physician versus those without COPD (37.2% vs 21.8%, respectively) and exacerbations doubled the number of visits. The prevalence of any medical visits to any physician was also higher in asthma patients versus those without asthma (wheezing: 47.2% vs 22.7%; medical diagnosis: 54.6% vs 21.6%; wheezing plus reversibility: 46.2% vs 23.8%, respectively). Asthma patients with exacerbations had twice the number of visits versus those without an exacerbation. The number of visits was higher (2.8 times) in asthma–COPD overlap, asthma (1.9 times), or COPD (1.4 times) patients versus those without these respiratory diseases; the number of visits due to exacerbation was also higher (4.9 times) in asthma–COPD overlap, asthma (3.5 times), and COPD (3.8 times) patients. Conclusion COPD, asthma, and asthma–COPD overlap increase the prevalence of medical visits and, therefore, health care resource utilization. Attempts to reduce health care resource use in these patients require interventions aimed at preventing exacerbations. PMID:27994446

  7. 2000 Census Public Use Microdata Area (PUMA) 5% for McKinley County, New Mexico, 2006se TIGER

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — The 2006 Second Edition TIGER/Line files are an extract of selected geographic and cartographic information from the Census TIGER database. The geographic coverage...

  8. 2000 Census Public Use Microdata Area (PUMA) 5% for De Baca County, New Mexico, 2006se TIGER

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — The 2006 Second Edition TIGER/Line files are an extract of selected geographic and cartographic information from the Census TIGER database. The geographic coverage...

  9. 2000 Census Public Use Microdata Area (PUMA) 1% for San Juan County, New Mexico, 2006se TIGER

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — The 2006 Second Edition TIGER/Line files are an extract of selected geographic and cartographic information from the Census TIGER database. The geographic coverage...

  10. 2000 Census Public Use Microdata Area (PUMA) 5% for San Juan County, New Mexico, 2006se TIGER

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — The 2006 Second Edition TIGER/Line files are an extract of selected geographic and cartographic information from the Census TIGER database. The geographic coverage...

  11. 2000 Census Public Use Microdata Area (PUMA) 5% for Los Alamos County, New Mexico, 2006se TIGER

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — The 2006 Second Edition TIGER/Line files are an extract of selected geographic and cartographic information from the Census TIGER database. The geographic coverage...

  12. 2000 Census Public Use Microdata Area (PUMA) 5% for Rio Arriba County, New Mexico, 2006se TIGER

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — The 2006 Second Edition TIGER/Line files are an extract of selected geographic and cartographic information from the Census TIGER database. The geographic coverage...

  13. 2000 Census Public Use Microdata Area (PUMA) 5% for San Miguel County, New Mexico, 2006se TIGER

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — The 2006 Second Edition TIGER/Line files are an extract of selected geographic and cartographic information from the Census TIGER database. The geographic coverage...

  14. 2000 Census Public Use Microdata Area (PUMA) 5% for Dona Ana County, New Mexico, 2006se TIGER

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — The 2006 Second Edition TIGER/Line files are an extract of selected geographic and cartographic information from the Census TIGER database. The geographic coverage...

  15. 2000 Census Public Use Microdata Area (PUMA) 1% for McKinley County, New Mexico, 2006se TIGER

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — The 2006 Second Edition TIGER/Line files are an extract of selected geographic and cartographic information from the Census TIGER database. The geographic coverage...

  16. 2000 Census Public Use Microdata Area (PUMA) 1% for Dona Ana County, New Mexico, 2006se TIGER

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — The 2006 Second Edition TIGER/Line files are an extract of selected geographic and cartographic information from the Census TIGER database. The geographic coverage...

  17. 2000 Census Public Use Microdata Area (PUMA) 1% for Santa Fe County, New Mexico, 2006se TIGER

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — The 2006 Second Edition TIGER/Line files are an extract of selected geographic and cartographic information from the Census TIGER database. The geographic coverage...

  18. 2000 Census Public Use Microdata Area (PUMA) 1% for San Miguel County, New Mexico, 2006se TIGER

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — The 2006 Second Edition TIGER/Line files are an extract of selected geographic and cartographic information from the Census TIGER database. The geographic coverage...

  19. 2000 Census Public Use Microdata Area (PUMA) 5% for Santa Fe County, New Mexico, 2006se TIGER

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — The 2006 Second Edition TIGER/Line files are an extract of selected geographic and cartographic information from the Census TIGER database. The geographic coverage...

  20. Psychosocial work characteristics as predictors for burnout: findings from 3-year follow up of the PUMA Study

    DEFF Research Database (Denmark)

    Borritz, Marianne; Bültmann, Ute; Rugulies, Reiner

    2005-01-01

    OBJECTIVE: The objective of this study was to investigate the impact of psychosocial work characteristics on burnout. METHODS: A total of 1772 participants in different human service sector organizations were eligible for the cross-sectional analyses (baseline) and 952 for the prospective analyse...

  1. 2000 Census Public Use Microdata Area (PUMA) 1% for Los Alamos County, New Mexico, 2006se TIGER

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — The 2006 Second Edition TIGER/Line files are an extract of selected geographic and cartographic information from the Census TIGER database. The geographic coverage...

  2. 2000 Census Public Use Microdata Area (PUMA) 1% for Rio Arriba County, New Mexico, 2006se TIGER

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — The 2006 Second Edition TIGER/Line files are an extract of selected geographic and cartographic information from the Census TIGER database. The geographic coverage...

  3. 2000 Census Public Use Microdata Area (PUMA) 1% for De Baca County, New Mexico, 2006se TIGER

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — The 2006 Second Edition TIGER/Line files are an extract of selected geographic and cartographic information from the Census TIGER database. The geographic coverage...

  4. Conexiones revolucionarias: repercusiones de la expropiación petrolera mexicana en Bolivia, 1938

    Directory of Open Access Journals (Sweden)

    María Cecilia Zuleta

    2014-11-01

    Full Text Available Este trabajo trata un tema relevante en la historiografía latinoamericana como es la expropiación petrolera mexicana durante el gobierno de Lázaro Cárdenas y aporta información original así como un enfoque innovador que desborda las historiografías estrictamente nacionales, intentando contribuir tanto a la historia de Bolivia como a la mexicana. La expropiación mexicana de 1938 alcanzó una dimensión trasnacional más allá de sus repercusiones diplomáticas: una experiencia percibida bajo muy diversos prismas en cada país latinoamericano. ¿Cómo fue vista la expropiación mexicana en los Andes bolivianos? Se argumenta que en la Paz, y en Sucre, diversos sectores de la sociedad leyeron esa expropiación tras los filtros del conflicto con la Standard Oil of Bolivia y del debate ideológico y político en torno a la recién creada YPFB, y cargaron de otro significado este episodio nacionalista al adaptarlo al contexto boliviano, dialogando simultáneamente con el sustrato previo de recepción de la revolución mexicana en la región, así como con el especial contexto político, económico y diplomático andino. Razones de orden nacional e internacional explican las reacciones ante el episodio petrolero mexicano en Bolivia, tanto como la percepción de estas por parte de la diplomacia mexicana.This article deals with a relevant subject in Latin American historiography, that being the Mexican oil expropriation during the Lázaro Cárdenas government and provides original information as well as an innovative approach that goes beyond strictly national historiographies, with the intention of contributing both to Bolivian and Mexican history. The Mexican expropriation of 1938 reached a transnational dimension beyond its diplomatic implications; an experience perceived through quite different prisms in each Latin American country. How was the Mexican expropriation viewed in the Bolivian Andes? We have argued that in La Paz and Sucre, various

  5. Remoción en masa y colapso catastrófico de diques naturales generados en el frente orogénico andino (36º-38ºs: los casos Navarrete y Río Barrancas

    Directory of Open Access Journals (Sweden)

    Ivanna M. Penna

    2008-06-01

    Full Text Available En los Andes nor-neuquinos, existe una gran concentración de depósitos de avalanchas de roca que generan endicamientos naturales. El colapso temprano de estos diques generados por deslizamientos de ladera es el proceso evolutivo más probable. Paleocostas, profundas y agudas brechas de colapso, y depósitos de outburst flood permiten reconstruir las características de las lagunas asociadas a los respectivos diques. En esta región, el último evento glaciario ha creado pendientes abruptas y fracturación interna de los materiales, los que determinaron un aumento de la susceptibilidad al colapso de las pendientes por factores externos como sismicidad o precitaciones. La distribución de las avalanchas aparenta estar asociada a la intersección entre fallas con actividad cuaternaria y los valles glaciarios. Este trabajo mostrará actividad neotectónica en el valle del arroyo Pichi- Neuquén, donde se encuentra el retrocorrimiento Aguas Calientes, que presenta estratos de crecimiento cuaternarios en el labio colgante de la falla. Adicionalmente, se observan probables sismitas en la desembocadura del arroyo Colorado. Este trabajo tiene como objetivo comparar el origen, similitud de inductores (probable actividad sísmica y ambiente geomorfológico de las avalanchas Río Barrancas y Navarrete, con énfasis en esta última. El primer dique natural mencionado ha colapsado catastróficamente (debido a un aumento del influjo de agua a la laguna luego de 2,2 Ka de existencia. En el segundo caso, la edad del depósito de avalancha y sus causas de colapso son aún inciertas. La existencia de un flujo de detritos en la margen este de la laguna Navarrete y la similar cota entre la paleocosta y el tope del dique, permiten proponer distintas hipótesis acerca de las causas de colapso del dique.

  6. Manuel Alcántara Saez, Flavia Freidenberg (eds.), Partidos políticos de América Latina. Países andinos, Salamanca, Ediciones Universidad, 2001, 680 pp.

    OpenAIRE

    Langue, Frédérique

    2005-01-01

    Centrado en la organización y el funcionamiento interno de los partidos y no tanto en el análisis "ambiental" o electoral que prevaleció en la historiografía especializada, esta recopilación hace hincapié en los partidos como sistemas y adelanta una propuesta de estudio al respecto. Al raíz de esta elección metodológica está la "revalorización del papel de los partidos en América Latina", propuesta que, desde luego, se tendría que relativizar según el país y la cronología considerada. "Mi......

  7. EVALUACIÓN DE DOS TRATAMIENTOS FOTOPERIÓDICOS EN CRISANTEMO (Dendranthema grandiflorum (Ramat. Kitam., BAJO CONDICIONES DEL INTERTRÓPICO ANDINO ALTO EVALUATION OF TWO PHOTOPERIOD TREATMENTS IN CHRYSANTHEMUM (Dendranthema grandiflorum ((Ramat. Kitam., UNDER CONDITIONS OF HIGH ANDEAN INTERTROPIC

    Directory of Open Access Journals (Sweden)

    Fáber de Jesús Chica Toro

    2005-12-01

    Full Text Available En la floricultura colombiana, cerca del 14 % de los costos de producción del crisantemo o pompón (Dendranthema grandiflorum (Ramat. Kitam., está representado por la luz complementaria requerida para controlar su floración. Con el fin de encontrar un protocolo que hiciera uso de un menor suministro de luz, en este trabajo se compararon los efectos de dos esquemas de complemento lumínico nocturno sobre algunas características precosecha y poscosecha en dos variedades comerciales tipo spider de crisantemo: Super White y Super Yellow. El esquema testigo de suplemento lumínico nocturno consistió en aportar la adición durante 28 días para la variedad Super Yellow, y 26 para la variedad Super White. La propuesta alternativa de adición lumínica nocturna consistió en interrumpir el estímulo durante una noche completa cada dos días. Tal disminución del estímulo lumínico, en aproximadamente un 30 %, no afectó el crecimiento ni las características poscosecha de las variedades evaluadas.In Colombian floriculture, nearly 14% of the production costs of chrysanthemum (Dendranthema grandiflorum (Ramat. Kitam. consists of the additional lighting required to control its flowering. In order to find a protocol that consumes a lower amount of electricity, this study compared the effects of two supplementary noctural lighting schemes on several preharvest and postharvest characteristics in two commercial varieties of chrysanthemum: Super White and Super Yellow. The control nocturnal lighting scheme consisted of providing the supplemental lighting during 28 days for the Super Yellow variety and during 26 days for the Super White variety. The alternative nocturnal lighting scheme consisted of interrupting the stimulus during one complete night every two days. This approximately 30% reduction in light stimulation did not affect either growth or postharvest characteristics of the varieties evaluated.

  8. Metamorfismo de muy bajo grado asociado a un sistema geotermal en rocas volcánicas básicas del Jurásico Medio, Formación Lonco Trapial, Paso Berwin, Chubut extra-andino

    Directory of Open Access Journals (Sweden)

    P. Montecinos

    2004-12-01

    Full Text Available Las rocas volcánicas básicas de la Formación Lonco Trapial, poseen una mineralogía primaria compuesta principalmente por olivino, plagioclasa cálcica y piroxeno, presentando además gran cantidad de amígdalas. La mineralogía metamórfica de muy bajo grado en estas rocas está caracterizada por ceolitas, minerales arcillosos y micáceos. La asociación mineral más característica está compuesta por celadonita, montmorillonita, heulandita, analcima, gonnardita y, además, por levyna y minerales de la serie analcima-wairakita en forma subordinada. Esta asociación pertenece a la facies ceolita y tuvo un origen asociado a un sistema geotermal. La analogía establecida con las zonas de ceolitas conocidas para sistemas geotermales equivalentes y el uso de la grilla petrogénetica para paragénesis ceolíticas, en el sistema An-Ab-SiO2-H2O, permiten establecer un rango de temperatura entre 80°-180°C. En este contexto habría ocurrido un único evento metamórfico hidrotermal en presencia de una fase fluida, muy pobre en CO2, cuya composición química habría cambiado desde un carácter neutro con baja a(SiO2 y a(K2O moderada (permitiendo la precipitación de esmectitas y celadonita a un carácter neutro-alcalino con a(SiO2 que posibilita la estabilidad de la heulandita.

  9. Ecomicrobiology and microbial assimilative capacity of the oligotrophic Andean Lake Laja, Chile Ecomicrobiología y capacidad asimilativa microbiana del lago oligotrófico andino Laja, Chile

    Directory of Open Access Journals (Sweden)

    BERNHARD KARRASCH

    2011-09-01

    Full Text Available A strong socio-economic development pressure in South Chile will more and more cause an impact to the present lakes and rivers. Nevertheless, our knowledge concerning the ecological structure and the microbial self-purification capabilities of these lacustrine water bodies is scant but essential for a future sustainable development of land and water use. We studied Lake Laja, a lake already heavily impacted by water diversions for hydropower generation and irrigation. Typical for the Andean region Lake Laja is an oligotrophic water body, limited by nitrogen nutrients. Only very low chlorophyll a and particulate organic carbon (POC concentrations and a small abundance and biomass of bacteria (mainly ultramicrobacteria and heterotrophic flagellates were encountered. Weak trophic interrelations were derived from a high bacteria-to-heterotrophic flagellate ratio. For the ten investigated extracellular enzymes (alkaline phosphatase, leucine, arginine-, glycine and tyrosine-aminopeptidase, α-, β-D-glucosidase, α-, β-D-galactosidase, N-acetyl-β-D-glucosaminidase, with the exception of α-D-glucosidase, a broad range of organic matter degradation activities was proven. Probably, due to the N-limitation, organic nitrogen hydrolysing extracellular enzymes reached activities on average of 45 % compared to other studies in oligotrophic waters. The possible effect of N-limitation on extracellular enzyme activities was more pronounced by cell specific extracellular enzymatic activity rates, which exceeded those of other oligotrophic water bodies on average by factor 2. The overall activities of all microbial extracellular enzymes studied proved to be dominated by the dissolved free external enzymes (up to 98 % over the ectoenzymes which are associated with particulate organic matter (bacterial cell walls, particles, and aggregates. It is concluded that future socio-economic changes, dealing with watershed human intervention (accelerating inorganic and organic loads as well as global change (temperature and precipitation changes could lead to significant changes in the ecology of Lake Laja.La gran presión generada por el desarrollo socioeconómico del sur de Chile, está causando cada vez más un mayor impacto en los lagos y ríos allí presentes. Sin embargo, el conocimiento de la estructura ecológica y la capacidad microbiana de autodepuración de los cuerpos de agua lacustres es escasa, pero esencial para un futuro uso sostenible de los recursos agua y suelo. Se estudió el lago Laja, por ser un cuerpo de agua muy afectado por la extracción de agua para la generación de energía hidroeléctrica y el riego. Como es característico de la región andina, el lago Laja es oligotrófico limitado por nutrientes nitrogenados. Se encontraron bajas concentraciones de clorofila a y carbono orgánico particulado (POC, así como bajas abundancias y biomasa de bacterias (principalmente ultramicrobacteria y de flagelados heterotróficos. Interrelaciones tróficas débiles se determinaron de la relación bacterias y flagelados heterotróficos. Para las diez enzimas extracelulares estudiadas (fosfatasa, leucina-, arginina-, glicina- y tirosina-aminopeptidasa, a-, b-D-glucosidasa, a-, b-D-galactosidasa, N-acetyl-b-D-glucosaminidasa, con la excepción de α-β-glucosidase, se analizó un amplio rango de actividad de degradación sobre la materia orgánica. Probablemente, debido a la limitación de nitrógeno, el N-orgánico hidrolizado por enzimas extracelulares alcanzó una actividad promedio de 45 %, comparada con otros estudios en aguas oligotróficas. El posible efecto de la limitación de N en la actividad enzimática extracelular, fue más pronunciado en las tasas de la actividad enzimática extracelular específicas, las cuales excedieron en un factor promedio de dos a las de otros cuerpos de aguas oligotróficos. En general, la actividad de todas las enzimas extracelulares microbianas estudiadas resultaron estar dominadas por enzimas externas disueltas libres (hasta el 98 %, más que por ectoenzimas asociadas a partículas de materia orgánica (paredes celulares de bacterias, partículas y agregados. De esta manera es posible concluir que los futuros cambios socioeconómicos, reflejados en el incremento de cargas orgánicas e inorgánicas, así como el efecto del cambio global (cambios en temperaturas y precipitaciones, podrían provocar cambios significativos en la ecología del lago Laja.

  10. Patrones de distribución espacial de ensambles de macroinvertebrados bentónicos de un sistema fluvial Andino Patagónico Spatial distribution patterns of benthic macroinvertebrates assemblages in an Andean Patagonian fluvial system

    Directory of Open Access Journals (Sweden)

    CAROLINA MOYA

    2009-01-01

    Full Text Available En enero de 2006 se estudiaron los patrones espaciales de distribución de comunidades de macroinvertebrados bentónicos de la cuenca hidrográfica del río Baker (45°50' O y 47°55' S y los principales factores controladores, intentando cubrir la mayor variedad de ecosistemas lóticos. Para llevar a cabo el estudio se seleccionaron 27 estaciones de muestreo ubicadas en las diferentes subcuencas del río. En cada estación se realizó una caracterización fisicoquímica del agua (conductividad, oxígeno disuelto, pH, temperatura y turbidez, y se documentaron las características del tramo de río (e.g. ancho del cauce y tipo de sedimento e información cartográfica utilizando un sistema de información geográfica (SIG. Se identificaron un total de 51 taxa que correspondieron en su mayoría a larvas de insectos (80 %. Los grupos con mayor riqueza fueron los órdenes Ephemeroptera (15 taxa, Plecoptera (8 taxa y Trichoptera (8 taxa. Los análisis de clasificación y ordenación realizados con los datos de abundancia, permitieron reconocer siete grupos de estaciones diferentes (A-F que fueron estadísticamente significativos (P In January of 2006 we studied the distributional patterns of benthic macroinvertebrate communities of the Baker river basin (45°50' O and 47°55' S and their main controlling factors trying to cover the greater variety of the lotic ecosystems. To carry out the study, 27 sampling stations were located in the different sub basins of the river. In each station, physical-chemical parameters of the column of water were quantified (conductivity, dissolved oxygen, pH, temperature and turbidity, and documented characteristics of the segment (e.g. wide of the channel and sediment type and cartographic information using a geographic information system (GIS and complemented with cartographic information using GIS. Identified a total of 51 taxa, are mostly insect larvae (80 %. The groups most richness were orders Ephemeroptera (15 taxa, Plecoptera (8 taxa and Trichoptera (8 taxa. The classification and ordination analyses carried out with the data of abundance, allowed to recognize seven groups stations (A-F statistically significant (P < 0.05. The múltiple regression analysis, showed that the environmental variable that accounts for the observed biological patterns, was coverage cobble, explaining 45.1 % the variance of the biological data (r² = 0.44 P < 0.05.

  11. El espacio de la identificación cultural. Notas sobre el nacimiento político del orden de la diversidad cultural en los países andinos (Ecuador y Perú

    Directory of Open Access Journals (Sweden)

    Silvia Rodríguez Maeso

    2006-09-01

    Full Text Available El artículo ofrece una reflexión teórica en torno a la génesis de un orden de representación de lo social, la diversidad cultural, en la región andina y al papel de las ciencias sociales en la misma. La centralidad de la configuración de identidades indígenas y de sus demandas políticas en América Latina, concretamente en el caso ecuatoriano, se presentan como un caso típico de lo culturalmente diverso. En el texto se argumenta que una mejor comprensión de estos procesos identitarios, así como de su ausencia, pasa por preguntarse por la configuración socio-histórica de distintas ideas de igualdad vinculadas a dos conceptos de cultura: la cultura como proceso civilizatorio y la cultura como diferencia. La argumentación teórica es ilustrada con el trabajo empírico realizado en espacios urbanos periféricos de las ciudades de Lima y Quito.

  12. El espacio de la identificación cultural. Notas sobre el nacimiento político del orden de la diversidad cultural en los países andinos (Ecuador y Perú

    Directory of Open Access Journals (Sweden)

    Silvia Rodríguez Maeso

    2006-01-01

    Full Text Available El artículo ofrece una reflexión teórica en torno a la génesis de un orden de representación de lo social, la diversidad cultural, en la región andina y al papel de las ciencias sociales en la misma. La centralidad de la configuración de identidades indígenas y de sus demandas políticas en América Latina, concretamente en el caso ecuatoriano, se presentan como un caso típico de lo culturalmente diverso. En el texto se argumenta que una mejor comprensión de estos procesos identitarios, así como de su ausencia, pasa por preguntarse por la configuración socio-histórica de distintas ideas de igualdad vinculadas a dos conceptos de cultura: la cultura como proceso civilizatorio y la cultura como diferencia. La argumentación teórica es ilustrada con el trabajo empírico realizado en espacios urbanos periféricos de las ciudades de Lima y Quito.

  13. Aproximación metagenómica para la identificación de enzimas lipolíticas en suelo de busque alto andino del parque nacional natural los nevados

    OpenAIRE

    Montaña Lara, Jose Salvador

    2015-01-01

    Las Enzimas lipolíticas son carboxil-ester-hidrolasas que rompen los enlaces éster de acilglicéridos mediante la adición de una molécula de agua, dando lugar a ácidos grasos libres y glicerol. Las lipasas se han convertido en uno de los principales biocatalizadores o bioaceleradores con potencial uso bioindustrial y su uso comercial es un negocio multimillonario que comprende una amplia variedad de aplicaciones, incluyendo la producción de biodiesel, especialidades farmacéuticas, agroquímicos...

  14. Diversidad de coleópteros en un bosque alto andino del municipio de Santa Rosa de Viterbo (Boyacá (Diversity of coleoptera in a high forest Andean in the municipality of Santa Rosa de Viterbo (Boyacá

    Directory of Open Access Journals (Sweden)

    Humberto Bohórquez Salazar

    2016-09-01

    Full Text Available Este artículo presenta los resultados de un estudio desarrollado en el municipio de Santa Rosa de Viterbo, ubicado a 2750 msnm, en un relicto de bosque húmedo montano bajo (bh-M. Dicho estudio tuvo como finalidad identificar la diversidad y riqueza de entomofauna referida al orden Coleóptera, en los predios de la Escuela de Policía Rafael Reyes (ESREY. Es importante señalar que este es el primer estudio de este tipo realizado en la zona. Se reportan un total de 270 individuos capturados pertenecientes a 17 familias, 25 subfamilias, 30 tribus y 44 géneros; siendo Coccinellidae la familia más representativa (19,3% y Dryophthoridae la familia menos representativa (0,4%. Vale la pena mencionar que, según lo reportado por Martínez (2005, no se encuentran registros de ningún género de Carabidae en el municipio, por lo que se puede destacar que los resultados publicados en este artículo son los primeros registros para Santa Rosa de Viterbo. Así mismo, la revisión de Medina, Lopera-Toro, Vitolo & Gill, (2001 sobre escarabajos coprófagos de Colombia indica que los géneros encontrados en este estudio no se registran a una altitud mayor a 2600 msnm por lo que se puede reportar un registro altitudinal para Canthon y Dichotomius Finalmente, en el artículo se presentan los resultados de un proceso de educación desarrollado con niños de grado quinto de primaria de una institución educativa del municipio y con los semilleros de investigación de la ESREY, proceso que fue diseñado como estrategia para promover la apropiación social de conocimiento y divulgación de los resultados del trabajo. (Abstract. This article presents the results of a study conducted in the municipality of Santa Rosa de Viterbo, located at 2750 meters above sea level, in a relict humid montane forest under (bh-M. This study aimed to identify the diversity and richness of Coleoptera order entomofauna referred to in the grounds of the School of Police Rafael Reyes (ESREY. It is important to note that this is the first study of its kind in the area. A total of 270 individuals captured belonging to 17 families, 25 subfamilies, 30 tribes and 44 genera are reported; Coccinellidae being the most representative (19.3% and less representative Dryophthoridae the family (0.4% family. It is important to note that, as reported by Martinez (2005, no records of any kind of Carabidae in the municipality are, so it can be noted that the results published in this article are the first records for Santa Rosa de Viterbo. Also the review of Medina, Lopera-Toro, Vitolo & Gill (2001 on dung beetles of Colombia indicate that genera found in this study are not recorded at a higher altitude to 2,600 meters so you can report altitudinal record for Canthon and Dichotomius Finally, the article presents the results of an education process developed with children in fifth grade of an educational institution of the municipality and the hotbeds of research ESREY, a process that was designed as a strategy to promote social appropriation of present knowledge and dissemination of the results of work.

  15. III.2. Ritual español, práctica indígena: la occidentalización del mundo andino a través del espectáculo de las instituciones coloniales1

    OpenAIRE

    Caillavet, Chantal

    2016-01-01

    1. Factores de aculturación En la América Latina colonizada, a partir del momento en que se produjo el contacto entre españoles y amerindios, la aculturación de los autóctonos se ha atribuido por lo general al predominio de dos factores esenciales: por un lado, la imposición consciente y deliberada por parte de los colonizadores españoles, de ideologías y normas de comportamiento; por otro, el contacto cotidiano con objetos importados de Europa, que influye fuertemente en la cultura material....

  16. Propiciando el encuentro: La estructuración de los paisajes de cacería en el contexto andino Propitiating the encounter: The structuration of hunting landscapes in the andean context

    Directory of Open Access Journals (Sweden)

    Enrique Moreno

    2012-11-01

    Full Text Available La cacería es una práctica social relevante para la reproducción social. Esta actividad sustentada en la apropiación de animales silvestres se basa en la articulación de una diversidad de aspectos, uno de los cuales es el paisaje. Los encuentros entre cazadores y presas se dan en un espacio significativo, donde las relaciones son vividas y experimentadas cotidianamente. Este morar en el paisaje donde se materializan los encuentros genera huellas que podemos identificar y registrar mediante metodologías propias de la arqueología, lo que nos permite de esta manera generar interpretaciones acerca de las prácticas cinegéticas a lo largo de la historia. En este sentido, presentaremos aquí los resultados obtenidos de prospecciones intensivas llevadas a cabo en la quebrada de Antofalla, departamento Antofagasta de la Sierra, provincia de Catamarca, tendientes a evaluar tanto las características del paisaje del área (fuentes de agua, laderas, altitudes sobre el nivel del mar, vegetación, etc. como las modificaciones realizadas por las poblaciones humanas. De esta manera, estructuras tales como trincheras, refugios o escondites generan, en conjunto con los rasgos topográficos del entorno, un paisaje que anticipa el encuentro entre cazador y presa, basado en los conocimientos y las relaciones entabladas entre los diferentes factores que participan de esta práctica.Hunting is a relevant social practice for social reproduction. This activity, based on the appropriation of wild animals, articulates a variety of elements, one of which is the landscape. Encounters between hunters and their prey take place in a significant space, where relations are lived and experienced daily. Dwelling in the landscape where the encounters take place generates traces that can be identified and recorded through archaeological methodologies, enabling the generation of interpretations about historical hunting practices. As such, results obtained from intensive surveys in the Quebrada de Antofalla, Departamento Antofagasta de la Sierra, Provincia de Catamarca are presented. The surveys aimed to assess both the characteristics of the landscape of the area (water sources, slopes, elevations above sea level, vegetation, etc. and changes made by human populations. In this way, structures such as trenches, shelters or hides, together with environmental features, generate a landscape which anticipates the encounter between hunter and prey through the knowledge and relations established among the various elements of the practice.

  17. School Children's Knowledge and Perceptions of Jaguars, Pumas, and Smaller Cats around a Mosaic of Protected Areas in the Western Brazilian Pantanal

    Science.gov (United States)

    Porfirio, Grasiela; Sarmento, Pedro; Fonseca, Carlos

    2014-01-01

    Surveys to assess environmental knowledge are elementary tools to ensure successful environmental education. Felines are considered key components of the environment, acting as flagships for conservation. Nevertheless, they are threatened by loss of habitat, prey reductions, and poaching. In the mosaic of protected areas in the Brazilian Pantanal,…

  18. Adidas ja Nike - nina vastu nina / Mikk Salu

    Index Scriptorium Estoniae

    Salu, Mikk, 1975-

    2005-01-01

    Spordikaupade tootja Adidas ostab Reeboki 3 miljardi euro eest. Adidase ja Reeboki ühinemisse suhtub skeptiliselt Puma, selle tegevjuht Jochen Zeitz lükkab tagasi kuuldused Nike'i ja Puma võimaliku ühinemise kohta

  19. The Copenhagen Burnout Inventory: A new tool for the assessment of burnout

    DEFF Research Database (Denmark)

    Kristensen, Tage S.; Borritz, Marianne; Villadsen, Ebbe

    2005-01-01

    Burnout; CBI; Copenhagen Burnout Inventory; exhaustion;fatigue; human service work; psychosocial work environment; PUMA study; questionnaire validity......Burnout; CBI; Copenhagen Burnout Inventory; exhaustion;fatigue; human service work; psychosocial work environment; PUMA study; questionnaire validity...

  20. Hvad velja nemendur ad syna okkur? (Islandsk)

    DEFF Research Database (Denmark)

    Kristensen, René

    2014-01-01

    Pædagogisk udredning med PUMA, et redskab udviklet af forfatteren til brug i specialundervisning og inklusion......Pædagogisk udredning med PUMA, et redskab udviklet af forfatteren til brug i specialundervisning og inklusion...

  1. Active orogeny of the south-central Andes studied with GPS geodesy Orogenia activa de los Andes centro-australes estudiada mediante geodesia de GPS

    Directory of Open Access Journals (Sweden)

    E. Kendrick

    2006-12-01

    cinturón montañoso andino. Se encuentra que esta segunda componente, por ejemplo en el desplazamiento actual relativo al cratón del antearco y los altos Andes, puede ser modelada muy bien como una rotación horaria constante de la microplaca andina alrededor de un polo de Euler localizado en el sur de Argentina. Cerca de Malargüe, esta microplaca (o bloque se mueve subparalelamente al rumbo del orógeno, transportando material hacia la inflexión de los Andes Centrales. Más al norte, en el sector austral de los Andes Centrales, el movimiento de este bloque es casi perpendicular al rumbo de la cadena montañosa. Se sugiere que las tasas de deformación permanente en el retroarco varían entre un máximo de ~ 6-7 mm/año en el Subandino Boliviano y menos de ~ 3 mm/año en la Precordillera Argentina y en la faja plegada de Malargüe. Es probable que la deformación activa más importante esté ocurriendo en una delgada faja (~ 50 km de ancho asociada con el límite del retroarco (usualmente definido como el frente orogénico. En esta etapa, es imposible distinguir si estructuras específicas del retroarco está acumulando deformación.

  2. Comparação entre duas concentrações de glicerol para a criopreservação de sêmen de suçuarana (Puma concolor Comparison between two glycerol concentrations to cryopreservation of semen of mountain lions (Puma concolor

    Directory of Open Access Journals (Sweden)

    Thyara de Deco-Souza

    2013-04-01

    Full Text Available O desenvolvimento de biotécnicas de reprodução é uma importante ferramenta para a conservação de animais silvestres ameaçados de extinção. Procedimentos de reprodução assistida em suçuarana, no entanto, são escassos na literatura, em especial aqueles relacionados à criopreservação de sêmen. Neste sentido, o presente trabalho objetivou avaliar a congelabilidade do sêmen de suçuaranas adultas mantidas em cativeiro, por meio da comparação entre duas concentrações de glicerol no meio de congelamento. Foram usados cinco machos adultos de suçuarana, mantidos no Centro de Reabilitação de Animais Silvestres do Mato Grosso do Sul (CRAS/MS. As amostras foram coletadas por eletroejaculação e avaliadas quanto ao seu aspecto físico, volume, vigor, motilidade, concentração e índice espermático. De cada ejaculado duas alíquotas foram diluídas em meio Tris-citrato-gema de ovo, em concentrações finais de 5 e 7,5% de glicerol, resfriadas a uma taxa de -0,55ºC/min e congeladas a uma taxa de -5,8ºC/min. Depois de descongeladas, as amostras foram reavaliadas e submetidas aos testes de termorresistência e hiposmótico. O protocolo de criopreservação e descongelamento de sêmen proposto se mostrou eficiente em ambas as concentrações de glicerol testadas, não havendo diferença (p>0,05 entre estas.The development of biotechnologies of reproduction is an important tool for the conservation of wild animals threatened with extinction. Assisted reproduction procedures in mountain lions, however, are scarce, especially those related to sperm cryopreservation. In this context, this study aimed to evaluate the freezing capacity of semen from adult mountain lions in captivity through the comparison of two concentrations of glycerol in the freezing media. Five adult male mountain lions were used, held at the Rehabilitation Center for Wild Animals of Mato Grosso do Sul (CRAS/MS. Samples were collected by electroejaculation and evaluated for physical appearance, volume, sperm progressive status, sperm motility, sperm concentration and sperm motility index. Each ejaculate was spliced into two aliquots and diluted in Tris-citrate-half egg yolk, at final concentrations of 5 and 7.5% glycerol, cooled at a rate of -0.55ºC/min and frozen at a rate of -5.8ºC/min. Once thawed, the samples were re-evaluated and tested for thermoresistance and hypoosmotic swelling. The suggested protocol for cryopreservation and thawing of semen is efficient in both glycerol concentrations tested, with no difference (p>0.05 between them.

  3. Un método para el análisis de frecuencia regional de lluvias máximas diarias: aplicación en los Andes bolivianos A method for regional frequency analysis of maximum daily rainfall: application in the Bolivian Andes

    Directory of Open Access Journals (Sweden)

    José Antonio Luna Vera

    2013-04-01

    Full Text Available Se presenta un análisis de frecuencia regional con series de lluvia diaria máxima anual para una zona con escasa información. La compleja orografía de montañas y el altiplano de una región en la cordillera de Los Andes, Bolivia, produce diferentes patrones de lluvia diaria. La combinación de los Momentos-L y el análisis de conglomerados resultan adecuados para identificarlas regiones homogéneas de las series máximas anuales. El trabajo desarrollado define 4 regiones homogéneas. La región 1 comprende las estaciones ubicadas en el altiplano y la zona Sur Este. La región 2 abarca el altiplano central y la cuenca del Río La Paz, compuesto por cuencas interandinas. La 3 delimita claramente las estaciones de la zona tropical amazónica; y la 4 está compuesta por estaciones ubicadas en las montañas del Norte. Se probaron diversas distribuciones para el análisis regional de frecuencias aplicando la técnica de estaciones-año; los mejores resultados se obtuvieron con las funciones Gumbel y Doble Gumbel. Finalmente se expresan las ecuaciones regionales y se comparan con algunas series puntuales de cada región, con el objeto de verificar la aplicabilidad de la metodología propuesta para fines de diseño hidrológico.A regional frequency analysis of daily annual maximum rainfall series for an area with poor information is presented. The complex topography mountains and the highlands region in the Cordillera de Los Andes, Bolivia, produce different patterns of daily rainfall. The combination of L-Moments and cluster analysis are adequate to identify the homogeneous regions of the annual maximum series. The work defines 4 homogeneous regions. Region 1 includes the stations located in the highlands and south-east. Region 2 covers the central highlands and La Paz River Basin, consisting of inter-Andean basins. Region 3 clearly defines the Amazonian basin stations and 4 is composed of stations located in the northern mountains. Different distributions were tested for regional frequency analysis using the Index Flood method, the best results were obtained with the Double Gumbel and Gumbel functions. Finally, regional equations are compared with some specified series of each region, in order to verify the applicability of the proposed methodology for hydrological design purposes.

  4. Radiation levels of radon gas and its progeny in labor activities of mine interior and their effects in the Bolivian workers to 4000 m; Niveles de radiacion de gas radon y su progenie en actividades laborales de interior mina y sus efectos en los trabajadores bolivianos a 4000 m

    Energy Technology Data Exchange (ETDEWEB)

    Mamani M, R.; Claros J, J. T.; Vasquez A, R.; Chirveches, H. [Universidad Autonoma Tomas Frias, Villa Imperial de Potosi (Bolivia, Plurinational State of); Weyer, J.; Arnold, D., E-mail: raulm2k13@hotmail.com [Universidad Tecnica de Freiberg, Academia de Minas, Akademiestrabe 6, 09599 Freiberg (Georgia)

    2014-08-15

    The objectives of this study are guided to determine the radiation charges to those that the mining workers are exposed, in order to assume the appropriate protection measures. The first phase of this study begun in March of 2009, with the support of the Freiberg University (Germany), has been preceded of different scientific events and preparatory measurements in the Universidad Autonoma Tomas Frias (UATF) and Cerro Rico de Potosi. Experimentally the presence of radon gas has been quantified by means of measurements in 7 mines of Cerro Rico de Potosi, the COMSUR mines (today SINCHY WAYRA) and the state mining company COMIBOL (Oruro-Huanuni), using the measurement equipment Alpha Zaeller-2 (AZ-2). The Radon-222 that emerges of the interior of the fractured rocks and/or loose rocks, with more intensity if they are fresh, is inhaled by the mining worker. Considering the breathing parameters (1, 2 m{sup 3}/h), recommended by the ICRP, was observed that the miners breathing of the region is more bigger, due to the high effort that they develop in the work. Therefore, the calculation results of the concentration of the radiation activity of Radon, allow assuming that this is the causing of the damage to the lungs in the human body. Outdoor or in superficial tunnels, the impact is low or imperceptible. But when the process is verified to more depths in an underground mine, or not properly ventilated, the concentration as well as the exposure dose to the radiation of radon gas is increased to amazing values. Assuming that the maximum permissible limit is of 20 mSv, the study has established some values that exceeded this limit in the Bolivian mining, not controlled in this field until now. The component of the medical study, through exams: hematology and spirometric, notes that, by the complex inhalation of: air + powders + Radon gas and others in his working environment, contribute strongly to the generation of lethal illnesses in the mining worker, as the Poliglobulia, Silicosis and the Lung Cancer. (Author)

  5. Con la secularización «se abrió el campo; el que quería venía». La formación de un grupo dirigente en el ámbito local boliviano, 1938/39-1948

    Directory of Open Access Journals (Sweden)

    García Jordán, Pilar

    2007-08-01

    Full Text Available The object of this article is to study the most significant changes that took place in Guarayos (NW of Santa Cruz Department, Bolivia, as a consequence of the secularization of the Franciscan Missions approved in 1938/39 and the implantation of the «Delegación Nacional de Guarayos», a state institution that was in charge of completing the incorporation to the «nation» of the Guarayos population. We will focus particularly on the «administradores», new «managers» of both territories and goods that through the connivance of their superiors and the incapacity of the State to set presence in the region, held complete power between 1939 and 1948 and were the main responsible actors of the economic decay and depopulation of the region.

    Objetivo del artículo es estudiar los cambios más significativos producidos en la región de Guarayos (Noroeste del departamento de Santa Cruz, Bolivia como consecuencia de la secularización de las misiones franciscanas en la zona, aprobada en 1938/39, y la implantación de la Delegación Nacional de Guarayos encargada de completar el proceso de incorporación de la población guaraya a la «nacionalidad». En particular, se analiza el papel desempeñado por los «administradores », nuevos «gestores» de los territorios y bienes que entre 1939 y 1948 detentaron todo el poder en cada uno de los poblados y fueron los responsables principales de la decadencia económica y despoblación de la región con la connivencia de sus superiores jerárquicos y la incapacidad del Estado para hacerse presente en la convulsa política boliviana del período marcada por continuados golpes militares.

  6. Evidencias sedimentarias y geoquímicas de la pequena edad de hielo en el lago Milluni Grande del Altiplano boliviano Sedimentary and geochemical evidences of the little Ice Age in the Milluni Grande Lake of the Bolivia high-platean

    Directory of Open Access Journals (Sweden)

    Matías Miguel Salvarredy-Aranguren

    2009-12-01

    Full Text Available El lago de Milluni Grande (LMG está ubicado en el flanco occidental de los Andes Orientales en el valle de Milluni que posee una clásica forma en U dado que se encuentra en una región glacial. El lago es el más grande de este valle, y dado que se halla situado al cierre de cuenca, resulta estratégico para albergar un registro sedimentario de los últimos 450 años. El registro sedimentario fue estudiado a partir de un testigo del lago, del cual se describieron y analizaron detalladamente parámetros físicos y químicos, sedimentología, mineralogía e indicadores geoquímicos los que permitieron evidenciar una importante variación del clima (VC. Adicionalmente, una datación con 210Pb en los niveles mas superficiales del testigo fue usada para establecer una tasa de sedimentación del lago y estimar una cronología para los niveles sin datación. La cronología definida señala que la variación del clima detectada sucede durante la Pequeña Edad de Hielo (LIA. Además, una correlación detallada fue establecida entre los niveles arenosos del registro sedimentario del LMG y las morrenas glaciarias del Cerro Charquini. Finalmente, algunas evidencias históricas bien documentadas sobre el desarrollo de Pequeña Edad de Hielo en Bolivia son mencionadas.Milluni Grande Lake (MGL is located Milluni Valley, west-side of Eastern Andes, Bolivia. The Milluni area presents a classic U shaped valley, product of the last glacial regression. MGL is the biggest lake of the valley, and it is at the end of Milluni Valley, a strategic position that allow a complete sedimentary record of last 450 years. The sediment record was studied with a lake core. A full description and analysis of physical and chemical parameters, sedimentology, mineralogy and geochemical proxys allow to conclude that sediment record shows an important climate change (CC. Complementary, a 210Pb datation over the superficial core levels was used to establish a sedimention rate and estimate a chronology for no dated levels. The chronology point out that the detected climate change clearly occurs during the Last Ice Age (LIA. Moreover, a detailed correlation has been established between sandy levels in the sedimentary record of MGL and Charquini mountain glacier moraines. Finally, some well documented facts about the development of Last Ice Age in this area of Bolivia are mentioned.

  7. Alexela välisomanik plaanib lisaraha kaasamist. Eesti on suure raha jaoks ebahuvitav / Hannes Sarv

    Index Scriptorium Estoniae

    Sarv, Hannes

    2011-01-01

    Alexela Logisticsi omanikeringi kuuluv Puma Energy plaanib minna börsile. Puma Energy äripartneri Heiti Hääle sõnul Puma oma tegevust Eestis laiendama ei tõtta, sest siin pole kuhugi investeerida. Raivo Vare kommentaare

  8. Promoción de la conservación de la biodiversidad mediante el aumento de la demanda interna de ajíes nativos en Bolivia

    Directory of Open Access Journals (Sweden)

    M. Olivera

    2013-01-01

    Full Text Available Bolivia es uno de los centros de diversidad de ajíes nativos (Capsicum spp.. El comercio informal de ajíes peruanos ha contribuido a una baja demanda y, por ende, a una reducción del cultivo (o conservación in situ de ajíes bolivianos. Este estudio analiza el consumo de ajíes mediante una encuesta a 359 hogares. Los resultados sugieren que los hogares están acostumbrados al sabor de los ajíes peruanos, y que entre las ventajas de los ajíes bolivianos está el color. Mezclas estandarizadas de ajíes con sabor y color deseados podrían introducirse satisfactoriamente al mercado, lo cual favorecería las ventas de ajíes bolivianos.

  9. Circuitos de la economía urbana en la ciudad de Buenos Aires: el comercio étnico de la colectividad boliviana en el barrio de Liniers

    Directory of Open Access Journals (Sweden)

    Mauro Escobar Basavilbaso

    2012-12-01

    Full Text Available Las divisiones territoriales del trabajo coexistentes en las ciudades pueden ser estudiadas como un circuito superior y un circuito inferior de la economía. El objetivo de este trabajo es comprender la convivencia de dichos circuitos en el barrio de Liniers (Ciudad Autónoma de Buenos Aires para analizar los rasgos que toma el circuito inferior y en particular, el comercio étnico boliviano. Las características del medio construido de Liniers y su situación de encrucijada y nudo del transporte de pasajeros lo convierten en un distrito comercial privilegiado con un enorme y fluido mercado. Sobresale allí el circuito inferior y en particular el comercio étnico boliviano. Para tal fin hemos utilizado estrategias cualitativas, tales como recorridos de visu, entrevistas a comerciantes argentinos y bolivianos.

  10. Espacios rurales y sociedades campesinas de una región andina

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    Cortes, Geneviève

    2015-01-01

    Lejos de ser homogéneo, el espacio rural boliviano comprende una gran diversidad de paisajes agrarios y de economías agrícolas. En este conjunto, las sociedades campesinas quechuas de la región de Cochabamba ocupan un lugar específico, tanto por su origen sociohistórico como por su economía rural. Las transformaciones económicas, políticas y sociales que han afectado al espacio rural boliviano a partir de los años cincuenta, fueron especialmente intensas en los valles de Cochabamba. Son ejemp...

  11. Identidades de diáspora a través de la danza folclórica: Un estudio ciberantropológico

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    Eveline Sigl

    2011-12-01

    Full Text Available El presente artículo explora el discurso sobre las danzas folclóricas bolivianas desde la perspectiva de diáspora tal como se presenta en la Web 2.0 y a través de ella. Metodológicamente se trata de un estudio ciberantropológico que investiga las implicaciones que tiene la danza folclórica no solo para los migrantes bolivianos y sus descendientes sino también para bailarines no bolivianos, y se enfoca especialmente en cuestiones de identidad y etnicidad.

  12. Concursos

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    Boletín Cultural y Bibliográfico Banco de la República

    1995-09-01

    Full Text Available Se presenta la información y las bases para los siguientes concursos; Concurso Enka de literatura infantil Premio Andino; Becas para Jóvenes Talentos en Música y Artes Plásticas.

  13. Aspectos do uso territorial por onça parda (Puma concolor, através de monitoramento via satélite, na região do Parque Estadual da Serra do Brigadeiro, MG

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    T.A.R. Paula

    2015-02-01

    Full Text Available Objetivou-se com o presente estudo monitorar o comportamento do uso territorial de um macho de onça parda, capturado no Parque Estadual da Serra do Brigadeiro (PESB, por meio da telemetria, usando colar de GPS via Satélite Globalstar. O monitoramento de doze meses resultou na transmissão via satélite de 328 localizações, as quais sugerem que a área utilizada por esse felino seja de aproximadamente 610km2, sendo esta a maior área já descrita para a espécie. Foi possível observar que apenas 20% das localizações ocorreram dentro da área do PESB. O comportamento territorial observado neste trabalho mostra a vulnerabilidade do animal a ações antrópicas, como a caça, atropelamentos e o contato com patógenos entre animais silvestres e domésticos. Observou-se também que duas rodovias configuram barreiras artificiais para o deslocamento do animal, possivelmente impedindo o fluxo gênico entre o PESB e dois outros importantes parques. Com os dados levantados é possível a sugestão de ações de manejo, como a criação de uma conectividade efetiva entre o PESB, o Parque Estadual do Rio Doce e o Parque Nacional do Caparaó, o que poderia contribuir para o intercâmbio genético entre as populações e, dessa forma, favorecer a conservação não somente da onça parda.

  14. Comparação entre duas concentrações de glicerol para a criopreservação de sêmen de suçuarana (Puma concolor

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    Thyara de Deco-Souza

    2013-04-01

    Full Text Available O desenvolvimento de biotécnicas de reprodução é uma importante ferramenta para a conservação de animais silvestres ameaçados de extinção. Procedimentos de reprodução assistida em suçuarana, no entanto, são escassos na literatura, em especial aqueles relacionados à criopreservação de sêmen. Neste sentido, o presente trabalho objetivou avaliar a congelabilidade do sêmen de suçuaranas adultas mantidas em cativeiro, por meio da comparação entre duas concentrações de glicerol no meio de congelamento. Foram usados cinco machos adultos de suçuarana, mantidos no Centro de Reabilitação de Animais Silvestres do Mato Grosso do Sul (CRAS/MS. As amostras foram coletadas por eletroejaculação e avaliadas quanto ao seu aspecto físico, volume, vigor, motilidade, concentração e índice espermático. De cada ejaculado duas alíquotas foram diluídas em meio Tris-citrato-gema de ovo, em concentrações finais de 5 e 7,5% de glicerol, resfriadas a uma taxa de -0,55ºC/min e congeladas a uma taxa de -5,8ºC/min. Depois de descongeladas, as amostras foram reavaliadas e submetidas aos testes de termorresistência e hiposmótico. O protocolo de criopreservação e descongelamento de sêmen proposto se mostrou eficiente em ambas as concentrações de glicerol testadas, não havendo diferença (p>0,05 entre estas.

  15. Modelo para la variación del volumen orogénico andino y acortamientos en el sector 20°-46°S Model for Andean orogenic volume variation and shortening in the 20°-46°S sector

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    F.A. Pose

    2005-12-01

    Full Text Available El volumen orogénico levantado en los Andes es el resultado del delicado equilibrio entre el levantamiento y la erosión. Los diferentes grados de acortamiento sufridos por el borde occidental del continente americano, desde el codo de Arica hasta Tierra del Fuego, han definido contrastantes rasgos morfotectónicos, desde extensas altiplanicies endorreicas hasta reducidas y simples divisorias. El volumen orogénico a través de los Andes Centrales Australes y Patagónicos Septentrionales (20°-46°S disminuye linealmente de norte a sur. Esta linealidad experimenta un drástico cambio de pendiente, a los 38°S, en la zona de transición entre los Andes Centrales Australes y los Patagónicos Septentrionales. La zona norte (20°-38°S presenta mayor velocidad de variación que la sur (38°-46°S. Este quiebre coincide con un cambio relativo en el gradiente de edades de fondo oceánico, sugiriendo un control en el acortamiento, al menos parcial, por parte de la flotabilidad de la placa oceánica subducida. Esto se contrapone con las hipótesis que postulan que el factor climático sería el principal condicionante de estos contrastes morfológicos. Se presentan además dos ecuaciones empíricas para la obtención de volúmenes orogénicos a una latitud dada, fuera del ámbito de dos anomalías centradas en 27° y 33°S respectivamente. A estas latitudes, otros factores adicionales al acortamiento orogénico están definiendo el relieve observado. Adicionalmente se discute una ley de variación del acortamiento en función de la latitud, calculado a partir de datos gravimétricos, fuera de los entornos de estas anomalías alrededor de las cuales se observan fluctuaciones equivalentes al caso anterior.The orogenic volume of the Andes is the result of the equilibrium between the tectonic uplift and erosion. The different rates of shortening along the western margin of the South American continent have defined contrasting morphostructural settings. The orogenic volume along the southern Central Andes and northern Patagonian Andes (20°-46°S decreases linearly, from north to south. At 38°S, we have separated two segments with different rates of variation in the Andean volume. The northern sector (20°-38°S shows a steeper linear law than the southern sector. Even though the Andean volume varies together with the decrease from north to south of the subducted oceanic slab age, latitude 38°S coincides with a sudden change related to this late parameter. This observation has led us to propose a linkage between subducted parameters and variation of Andean volume, which contrasts with other hypotheses that assume that climate, is the principal cause for the variations in the observed orogenic volume. We also present two empiric equations to calculate the orogenic volume at determined latitude, beyond the area of influence of two anomalies centered around 27°S and 33°S respectively. Those sectors are considered to be influenced by other factors related to isostatic rebounds, superposed to shortening. Additionally, we discuss a law for the latitudinal variation of the orogenic shortening away from the location of the mentioned anomalies.

  16. Cargas subcorticales en el antepaís andino y la planicie pampeana: evidencias estratigráficas, topográficas y geofísicas Subcrustal loads in the Andean foreland and pampean plain: stratigraphic, topographic and geophysical evidence

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    F.M. Dávila

    2005-12-01

    Full Text Available En la región sur de los Andes Centrales, la generación de espacios de acomodación en el antepaís ha sido explicada mediante la influencia de cargas supracorticales (apilamiento de escamas tectónicas y de cargas litostáticas. Estudios en la región de Sierras Pampeanas sugieren un patrón de subsidencia de longitud de onda regional (>500 km, difícilmente explicada por estas cargas generalmente asociadas a subsidencias de longitud de onda corta (In the Central Andes, supracrustal tectonic loading has been extensively invoked to explain retroarc flexure and foreland basin subsidence, due to stacking of tectonic slivers and amplification by sedimentary loads. However, we present surface and subsurface stratigraphic data from the Sierras Pampeanas broken foreland that suggest a long-wavelength subsidence pattern (>500 km, which is difficult to explain with upper crustal loads that are generally associated with short wavelength (< 300 km subsidence. It is proposed that stratigraphic, structural, topographic and geophysical features in the Sierras Pampeanas region and Chaco-Paranense basin can be explained by flections driven by processes occurring in the asthenosphere and/or lower crust, like thermal-driven corner mantle flow and/or eclogitization, respectively. Short-wavelength flexure (<300 km driven by various factors enhances regional subsidence features.

  17. Remoción en masa y colapso catastrófico de diques naturales generados en el frente orogénico andino (36º-38ºs: los casos Navarrete y Río Barrancas Mass wasting and catastrophic collapse of natural dams in the Andean orogenic front (36º-38ºS: The Navarrete and Río Barrancas case studies

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    Ivanna M. Penna

    2008-06-01

    Full Text Available En los Andes nor-neuquinos, existe una gran concentración de depósitos de avalanchas de roca que generan endicamientos naturales. El colapso temprano de estos diques generados por deslizamientos de ladera es el proceso evolutivo más probable. Paleocostas, profundas y agudas brechas de colapso, y depósitos de outburst flood permiten reconstruir las características de las lagunas asociadas a los respectivos diques. En esta región, el último evento glaciario ha creado pendientes abruptas y fracturación interna de los materiales, los que determinaron un aumento de la susceptibilidad al colapso de las pendientes por factores externos como sismicidad o precitaciones. La distribución de las avalanchas aparenta estar asociada a la intersección entre fallas con actividad cuaternaria y los valles glaciarios. Este trabajo mostrará actividad neotectónica en el valle del arroyo Pichi- Neuquén, donde se encuentra el retrocorrimiento Aguas Calientes, que presenta estratos de crecimiento cuaternarios en el labio colgante de la falla. Adicionalmente, se observan probables sismitas en la desembocadura del arroyo Colorado. Este trabajo tiene como objetivo comparar el origen, similitud de inductores (probable actividad sísmica y ambiente geomorfológico de las avalanchas Río Barrancas y Navarrete, con énfasis en esta última. El primer dique natural mencionado ha colapsado catastróficamente (debido a un aumento del influjo de agua a la laguna luego de 2,2 Ka de existencia. En el segundo caso, la edad del depósito de avalancha y sus causas de colapso son aún inciertas. La existencia de un flujo de detritos en la margen este de la laguna Navarrete y la similar cota entre la paleocosta y el tope del dique, permiten proponer distintas hipótesis acerca de las causas de colapso del dique.A high concentration of rock avalanches, most of them resulting in natural dams, is possible to recognize in the Andes of the northern Neuquén. In landslide dams the most probable evolutionary process corresponds to their early collapses. Paleoshorelines, deep and narrow breaches, and outburst flood deposits permit to rebuild the characteristics of the related lakes. The last glaciation in this region, have created steep slopes and physical rock weathering. These facts determined an increase in the susceptibility to slope failure triggered by seismicity and precipitation. The distribution of rock avalanches seems to be related to the interaction between young deformation and the glacial valleys. The Aguas Calientes backthrust in the Pichi-Neuquén valley is associated with Pliocene to Quaternary growth-strata, and probable seismic triggered liquefaction of sediments in the Colorado creek, and has evidence of neotectonic activity in the entire study area. The aim of this work is to compare the Río Barrancas and Navarrete rock avalanches, with special emphasis in the last one, and establish similitude in their triggering factor (probable seismic activity and geomorphic environment. The Río Barrancas dam has collapsed (due to an increase in the water influx into the lake after 2,200 years of existence. For the Navarrete case, the age of slope failure and causes of the collapse of the natural dam are still unknown. The presence of a debris flow in the eastern margin of the lake, and the similar altitude between the paleoshoreline and the top of the dam, would permit to propose two different scenarios.

  18. Effect of the time of use in the physical conditions of the an Andean soil in the department of Nariño, Colombia Efecto del tiempo de uso en las condiciones físicas del suelo andino en el departamento de Nariño, Colombia

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    Volverás Belisario

    2007-03-01

    Full Text Available Using a rainfall simulator (CIAT, changes in the soil loss and some physical properties associated to water movement by effect of the type of use of the soil through the time of use in the following treatments were evaluated: Non intervened soil with 25% slope (control, a pasture for 25 years and 25% slope and cropping wheat-corn (w-c of 25, 45 and 70 years of use, each one of these last, with 12% and 25% slope in a Inceptisol of the area of reconversión of the wheat crop of Nariño, Colombia (longitude 75º 14´ west, latitude 0.5º 10´ north, 2710 masl, 795 HR, 12ºC. The dynamics of the soil loss and the infiltration processes and movement of the water on the soil surface for simulated rain of 110 mm h-1 showed that the rate of soil loss, was minimum in the control and the pasture and was increased drastically until the 25 years of agricultural use (rotation w-c, time after which diminishes gradually when increasing the time of use of the soil. Of the applied total water sheet, the control and the pasture infiltrate 98,3% and 94% respectively; after 25, 45 and 70 years of agricultural use (rw-c, only 56%, 48% and 35% of infiltration respectively was detected. The change of agricultural use (rw-c generated increase of the apparent soil density and decrease of the clay percentage and organic matter when increasing the intensity and the time of use of soil. Key words: Soil loss, Infiltration, Andean hillsides, wheat, NariñoMediante un minisimulador de lluvia se evaluaron los cambios en pérdida de suelo y en algunas propiedades físicas asociadas con el movimiento del agua, en un Inceptisol (Dystric haplustepts de la zona de reconversión del cultivo de trigo del departamento de Nariño, Colombia (75º 14´ oeste, 0.5º 10´ norte, 2.710 msnm, 79% HR, 12ºC. Los tratamientos fueron: Suelo no intervenido a 25% de pendiente (testigo, uso en pastura por 25 años, 25% de pendiente y uso agrícola rotación trigo-maíz (rt-m de 25, 45 y 70 años de uso, cada uno de estos últimos a 12% y 25% de pendiente. La dinámica de la pérdida de suelo y de los procesos de infiltración y escorrentía mostró que la tasa de pérdida de suelo fue mínima en el testigo y la pastura y se incrementó drásticamente hasta los 25 años de uso agrícola, tiempo después del cual disminuyó paulatinamente con el aumento del tiempo de uso del suelo. De la lámina total aplicada el testigo y la pastura infiltraron el 98.3% y 94% respectivamente; después de 25, 45 y 70 años de uso agrícola se infiltró solo el 56%, 48% y 35% respectivamente. El cambio a uso agrícola (rt-m aumentó la densidad aparente y disminuyó el porcentaje de arcilla y de materia orgánica en la medida que aumentó la intensidad y el tiempo de uso del suelo. Palabras claves: Pérdida de suelo, Infiltración, laderas andinas, trigo, Nariño.

  19. Changes of sub-fossil chironomid assemblages associated with volcanic sediment deposition in an Andean lake (38°S, Chile Cambios en las asociaciones sub-fósiles de quironómidos, producto de la depositación de sedimentos volcánicos en un lago andino (38°S, Chile

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    ALBERTO ARANEDA

    2007-06-01

    Full Text Available Chironomid assemblages and sedimentological parameters (grain size, organic content, mineralogy of a short sediment core from Lake Galletué (38°41' S, 71°17' W were analysed. The sedimentary record includes one volcanic ash (tephra layer, which has a completely different composition than the host sediment in terms of organic content, grain size, and mineralogy. According to the geochronology (210Pb and 137Cs, this ash layer corresponds to the eruption of Llaima Volcano in 1956-1957. The tephra deposition had an impact on chironomid assemblages producing, among other changes, an increase in Parakiefferiella and a decrease in Ablabesmyia, although no noticeable change was detected in the diversity index. When compared with other studies, our results also show an impact in terms of the presence of chironomid head capsules within the tephra. The presence of these head capsules could result from the effects of percolation, since the coarse grain size of the tephra particles provides large interstitial spaces. The recovery in the abundances of some taxa after the tephra input, suggests the lake is probably restoring the conditions prevailing before the tephra fallSe analizan las asociaciones de restos sub-fósiles de quironómidos y los parámetros sedimentológicos de un núcleo sedimentario del lago Galletué (38°41' S, 71°17' O. Es evidente en este núcleo la presencia de un estrato de sedimentos volcánicos (tefra de una composición distinta en el contenido deµmateria orgánica, en el tamaño de partícula y en la mineralogía, respecto de los sedimentos del lago. De acuerdo a la geocronología isotópica (210Pb y 137Cs, los sedimentos de esta tefra podrían corresponder a la erupción del volcán Llaima de 1956-1957. Por otra parte la depositación de esta tefra también provocó un cambio en las asociaciones de quironómidos, siendo evidente el aumento de Parakiefferiella y la disminución de Ablabesmyia dentro del estrato de tefra. Si bien nuestros resultados, al igual que estudios previos, registran un cambio en las asociaciones de quironómidos debido a la entrada de sedimentos volcánicos, un aspecto diferente es la presencia de cápsulas cefálicas dentro de la tefra. Una posible explicación a tal diferencia podría ser la percolación de los restos de quironómidos por los espacios intersticiales de la tefra. La recuperación en la abundancia de algunos taxa luego de la depositación de la tefra, sugiere que el lago restablecería las condiciones existentes previo a la caída de la tefra

  20. Metamorfismo de muy bajo grado asociado a un sistema geotermal en rocas volcánicas básicas del Jurásico Medio, Formación Lonco Trapial, Paso Berwin, Chubut extra-andino Very low grade metamorphism associated with a geothermal system in basic volcanic rocks of the Middle Jurassic, Lonco Trapial Formation, Paso Berwin, Extra-Andean Chubut

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    P. Montecinos

    2004-12-01

    Full Text Available Las rocas volcánicas básicas de la Formación Lonco Trapial, poseen una mineralogía primaria compuesta principalmente por olivino, plagioclasa cálcica y piroxeno, presentando además gran cantidad de amígdalas. La mineralogía metamórfica de muy bajo grado en estas rocas está caracterizada por ceolitas, minerales arcillosos y micáceos. La asociación mineral más característica está compuesta por celadonita, montmorillonita, heulandita, analcima, gonnardita y, además, por levyna y minerales de la serie analcima-wairakita en forma subordinada. Esta asociación pertenece a la facies ceolita y tuvo un origen asociado a un sistema geotermal. La analogía establecida con las zonas de ceolitas conocidas para sistemas geotermales equivalentes y el uso de la grilla petrogénetica para paragénesis ceolíticas, en el sistema An-Ab-SiO2-H2O, permiten establecer un rango de temperatura entre 80°-180°C. En este contexto habría ocurrido un único evento metamórfico hidrotermal en presencia de una fase fluida, muy pobre en CO2, cuya composición química habría cambiado desde un carácter neutro con baja a(SiO2 y a(K2O moderada (permitiendo la precipitación de esmectitas y celadonita a un carácter neutro-alcalino con a(SiO2 que posibilita la estabilidad de la heulandita.The basic volcanic rocks of the Lonco Trapial Formation, have a primary mineralogy mainly composed of olivine, calcic plagioclase and pyroxene, and present a large quantity of amygdales. Their metamorphic mineralogy is characterized by zeolites, clay and micaceous minerals. The main mineral association includes celadonite, montmorillonite, heulandite, analcime, gonnardite and subordinate levyne and minerals of the wairakite-analcime series. This association belongs to the zeolite facies and its origin was associated with a geothermal system. The analogy with known zeolite zones in equivalent geothermal systems and the use of the petrogenetic grid for zeolite parageneses, in the system An-Ab-SiO2-H2O, permits to establish a range of temperature between 80°-180°C. A single metamorphic hidrothermal event would have taken place in presence of a CO2 very poor fluid phase, whose chemical composition possibly changed from a neutral character with low a(SiO2 and moderate a(K2O (allowing precipitation of smectites and celadonite to a neutral-alkaline character with high a(SiO2 that allows heulandite to be stable.