WorldWideScience

Sample records for andean crop mirabilis

  1. 77 FR 31039 - Andean Trade Preference Act: Impact on the U.S. Economy and on Andean Drug Crop Eradication

    Science.gov (United States)

    2012-05-24

    .... 332-352, Andean Trade Preference Act: Impact on the U.S. Economy and on Andean Drug Crop Eradication... INTERNATIONAL TRADE COMMISSION [Investigation No. 332-352] Andean Trade Preference Act: Impact on the U.S. Economy and on Andean Drug Crop Eradication AGENCY: United States International Trade...

  2. 75 FR 24967 - Andean Trade Preference Act: Impact on the U.S. Economy and on Andean Drug Crop Eradication

    Science.gov (United States)

    2010-05-06

    ... Preference Act: Impact on the U.S. Economy and on Andean Drug Crop Eradication. DATES: June 24, 2010... INTERNATIONAL TRADE COMMISSION [Investigation No. 332-352] Andean Trade Preference Act: Impact on the U.S. Economy and on Andean Drug Crop Eradication AGENCY: United States International Trade...

  3. 77 FR 28620 - Andean Trade Preference Act: Impact on the U.S. Economy and on Andean Drug Crop Eradication

    Science.gov (United States)

    2012-05-15

    ... INTERNATIONAL TRADE COMMISSION [Investigation No. 332-352] Andean Trade Preference Act: Impact on the U.S. Economy and on Andean Drug Crop Eradication AGENCY: United States International Trade... to report biennially to the Congress by September 30 of each reporting year on the economic impact of...

  4. Lost crops of the Incas: Origins of domestication of the Andean pulse crop tarwi, Lupinus mutabilis.

    Science.gov (United States)

    Atchison, Guy W; Nevado, Bruno; Eastwood, Ruth J; Contreras-Ortiz, Natalia; Reynel, Carlos; Madriñán, Santiago; Filatov, Dmitry A; Hughes, Colin E

    2016-09-01

    The Andean highlands are a hotspot of domestication, yet our understanding of the origins of early Andean agriculture remains fragmentary. Key questions of where, when, how many times, and from what progenitors many Andean crops were domesticated remain unanswered. The Andean lupine crop tarwi (Lupinus mutabilis) is a regionally important pulse crop with exceptionally high seed protein and oil content and is the focus of modern breeding efforts, but its origins remain obscure. A large genome-wide DNA polymorphism data set was generated using nextRADseq to infer relationships among more than 200 accessions of Andean Lupinus species, including 24 accessions of L. mutabilis and close relatives. Phylogenetic and demographic analyses were used to identify the likely progenitor of tarwi and elucidate the area and timing of domestication in combination with archaeological evidence. We infer that tarwi was domesticated once in northern Peru, most likely in the Cajamarca region within, or adjacent to the extant distribution of L. piurensis, which is the most likely wild progenitor. Demographic analyses suggest that tarwi split from L. piurensis around 2600 BP and suffered a classical domestication bottleneck. The earliest unequivocal archaeological evidence of domesticated tarwi seeds is from the Mantaro Valley, central Peru ca. 1800 BP. A single origin of tarwi from L. piurensis in northern Peru provides a robust working hypothesis for the domestication of this regionally important crop and is one of the first clear-cut examples of a crop originating in the highlands of northern Peru. © 2016 Botanical Society of America.

  5. Biodegradability and mechanical properties of starch films from Andean crops.

    Science.gov (United States)

    Torres, F G; Troncoso, O P; Torres, C; Díaz, D A; Amaya, E

    2011-05-01

    Different Andean crops were used to obtain starches not previously reported in literature as raw material for the production of biodegradable polymers. The twelve starches obtained were used to prepare biodegradable films by casting. Water and glycerol were used as plasticizers. The mechanical properties of the starch based films were assessed by means of tensile tests. Compost tests and FTIR tests were carried out to assess biodegradability of films. The results show that the mechanical properties (UTS, Young's modulus and elongation at break) of starch based films strongly depend on the starch source used for their production. We found that all the starch films prepared biodegrade following a three stage process and that the weight loss rate of all the starch based films tested was higher than the weight loss rate of the cellulose film used as control. Copyright © 2011 Elsevier B.V. All rights reserved.

  6. Genetic diversity and germplasm conservation of three minor Andean tuber crop species

    Directory of Open Access Journals (Sweden)

    Malice M.

    2009-01-01

    Full Text Available In traditional Andean agrosystems, three minor tuber crop species are of regional or local importance: oca (Oxalis tuberosa Molina, ulluco (Ullucus tuberosus Caldas and mashua (Tropaeolum tuberosum Ruiz and Pav.. Genetic diversity within these species is very large and could result from the high ecological and cultural variability that characterizes the Andean area. Nowadays, many anthropic or ecological factors cause the loss of diversity and contribute to genetic erosion. The development of conservation strategies for genetic resources of Andean tubers, in situ as well as ex situ, includes a better knowledge of diversity in addition to the study of Andean farming strategies linked to this genetic diversity.

  7. [Elaboration and evaluation of infant food based on Andean crops].

    Science.gov (United States)

    Repo-Carrasco, R; Hoyos, N L

    1993-06-01

    The Andes mountain range of South America is one of the most important centres for crop domestication, potato, corn, and lesser known grains such as quinua, cañihua, kiwicha and tarwi are indigenous of these highlands. These Andean grains have adapted perfectly to the climatic and geographical conditions present, whereas other grains have not been able to survive. In addition to their hardiness, they also have a high nutritional value. Bearing in mind on one hand, the high nutritional value of these indegenous products, and on the other hand the high rate of child malnutrition prevalent in the population, it was considered important to look for new variations in their processing which would facilitate their consumption by the poor working classes, especially the children. Accordingly three different flour mixtures were developed based on these Andean grains, the mixtures were then subjected to bromatological and biological analysis. The three new flour mixtures were: Quinua-Cañihua-Broad Bean (Q-C-B), Quinua-Kiwicha-Bean (Q-K-B) and Kiwicha-Rice (K-R). The protein content of these mixtures varied between 11.35-15.46 g/100g, the mixture K-R having the lowest protein level and the Q-C-B having the highest. The Q-K-B mixture had the highest chemical score, PER and NPU value. This PER value of 2.59 was higher than the value of casein which was 2.50. In addition this mixture had a chemical score of 0.94 and a NPU value of 59.38. The Q-C-B mixture had a chemical score of 0.88 and its PER, NPU and Digestibility values were 2.36, 47.24 and 79.2 respectively.(ABSTRACT TRUNCATED AT 250 WORDS)

  8. The diet of the black widow spider Latrodectus mirabilis (Theridiidae in two cereal crops of central Argentina

    Directory of Open Access Journals (Sweden)

    Gabriel Pompozzi

    2013-12-01

    Full Text Available The spider Latrodectus mirabilis (Holmberg, 1876 is commonly found in cereals crops of central Argentina. We studied its diet composition at the field and capture rate on leaf-cutting ants based on laboratory experiments. This study comprises the first approach that documents the diet of L. mirabilis in wheat and oat fields of central Argentina. We identified 1,004 prey items collected from its webs during the last phenological stages of both cereal crops. The prey composition was variable but the spiders prey mainly on ants (Formicidae, Hymenoptera, who represented more than 86% of the total. Meanwhile, in the capture rate experiences we registered a high proportion of ants captured by spiders at the beginning of experiences, capturing the half of the ants from total in the first four hours. Summarizing, we reported a polyphagous diet of this spider species in wheat and oat fields. Ants were the most important prey item of this spider, as found in other Latrodectus spiders around the world.

  9. Forecasting Andean rainfall and crop yield from the influence of El Nino on Pleiades visibility

    Science.gov (United States)

    Orlove; Chiang; Cane

    2000-01-06

    Farmers in drought-prone regions of Andean South America have historically made observations of changes in the apparent brightness of stars in the Pleiades around the time of the southern winter solstice in order to forecast interannual variations in summer rainfall and in autumn harvests. They moderate the effect of reduced rainfall by adjusting the planting dates of potatoes, their most important crop. Here we use data on cloud cover and water vapour from satellite imagery, agronomic data from the Andean altiplano and an index of El Nino variability to analyse this forecasting method. We find that poor visibility of the Pleiades in June-caused by an increase in subvisual high cirrus clouds-is indicative of an El Nino year, which is usually linked to reduced rainfall during the growing season several months later. Our results suggest that this centuries-old method of seasonal rainfall forecasting may be based on a simple indicator of El Nino variability.

  10. Study of Maca (Lepidium meyenii Walp., Andean crop with therapeutic properties

    Directory of Open Access Journals (Sweden)

    Gabriel Sifuentes-Penagos

    2015-06-01

    Full Text Available The maca is a native product of the Central Andes of Peru, it is resistant to hail, to frost and to prolonged droughts. It is cultivated from the Inca period in altitudes between 3800 - 4500 meters above sea level. This herbaceous plant, has not only a high nutritional value, but it is also valued for its medicinal role. Among the chemical components of this andean crop that have been related with therapeutic actions the increasing fertility and the energy levels, the antioxidant actions, the improving sexual desire and the growth rate. There are glucosinolates, sterols, fatty acids (macaene and their corresponding amides (macamides, alkaloids (lepidilines A and B, macaridine and polyphenols. This article presents a compilation of the researches that has been performed on the therapeutic properties of maca and its compounds responsible of them.

  11. Ecological and socio-cultural factors influencing in situ conservation of crop diversity by traditional Andean households in Peru

    Directory of Open Access Journals (Sweden)

    Velásquez-Milla Dora

    2011-12-01

    Full Text Available Abstract Background The Peruvian Andean region is a main center of plant domestication of the world. There, several tuber species were domesticated and the area lodges one of the most important reservoirs of their varieties and wild relatives. It is also the setting of traditional cultures using and conserving them. However, crop genetic erosion has been reported in the region since several decades ago; therefore, understanding factors influencing both loss and maintenance of crop variation is relevant to design conservation policies. Previous researches have examined factors influencing agrobiodiversity conservation in the region but additional case studies are recognized to be still necessary for a deeper understanding of causes of genetic erosion and for policy design to prevent and remedy it. Our study focused on analyzing (1 variation in richness of traditional varieties of tubers cultivated among households, (2 changes in varieties richness occurred in four consecutive agricultural cycles, and (3 ecological, social, and cultural factors influencing loss and conservation of varieties. Methods Richness of farmer varieties of tuber species cultivated by 28 peasant households was monitored in communities of Cajamarca and Huánuco, Peru during four consecutive agricultural cycles (from 2001 to 2005. In-depth interviews were conducted with 12 of the households with higher reputation as conservationists, in order to document farmers' perception of tubers qualities in ecological, social, economic, technological and culinary aspects and how these influence their decisions of conservation priorities. Traditional varieties were identified according to their local names, which were then confronted among farmers and with scientific catalogues in order to identify synonyms. Based on the information documented, indexes of ecological and socio-cultural factors affecting agricultural practices were designed, and their linear correlations and multivariate

  12. Ecological and socio-cultural factors influencing in situ conservation of crop diversity by traditional Andean households in Peru.

    Science.gov (United States)

    Velásquez-Milla, Dora; Casas, Alejandro; Torres-Guevara, Juan; Cruz-Soriano, Aldo

    2011-12-06

    The Peruvian Andean region is a main center of plant domestication of the world. There, several tuber species were domesticated and the area lodges one of the most important reservoirs of their varieties and wild relatives. It is also the setting of traditional cultures using and conserving them. However, crop genetic erosion has been reported in the region since several decades ago; therefore, understanding factors influencing both loss and maintenance of crop variation is relevant to design conservation policies. Previous researches have examined factors influencing agrobiodiversity conservation in the region but additional case studies are recognized to be still necessary for a deeper understanding of causes of genetic erosion and for policy design to prevent and remedy it. Our study focused on analyzing (1) variation in richness of traditional varieties of tubers cultivated among households, (2) changes in varieties richness occurred in four consecutive agricultural cycles, and (3) ecological, social, and cultural factors influencing loss and conservation of varieties. Richness of farmer varieties of tuber species cultivated by 28 peasant households was monitored in communities of Cajamarca and Huánuco, Peru during four consecutive agricultural cycles (from 2001 to 2005). In-depth interviews were conducted with 12 of the households with higher reputation as conservationists, in order to document farmers' perception of tubers qualities in ecological, social, economic, technological and culinary aspects and how these influence their decisions of conservation priorities. Traditional varieties were identified according to their local names, which were then confronted among farmers and with scientific catalogues in order to identify synonyms. Based on the information documented, indexes of ecological and socio-cultural factors affecting agricultural practices were designed, and their linear correlations and multivariate relations with varieties richness managed per

  13. Ocatin. A novel tuber storage protein from the andean tuber crop oca with antibacterial and antifungal activities.

    Science.gov (United States)

    Flores, Teresita; Alape-Girón, Alberto; Flores-Díaz, Marietta; Flores, Hector E

    2002-04-01

    The most abundant soluble tuber protein from the Andean crop oca (Oxalis tuberosa Mol.), named ocatin, has been purified and characterized. Ocatin accounts for 40% to 60% of the total soluble oca tuber proteins, has an apparent molecular mass of 18 kD and an isoelectric point of 4.8. This protein appears to be found only in tubers and is accumulated only within the cells of the pith and peridermis layers (peel) of the tuber as it develops. Ocatin inhibits the growth of several phytopathogenic bacteria (Agrobacterium tumefaciens, Agrobacterium radiobacter, Serratia marcescens, and Pseudomonas aureofaciens) and fungi (Phytophthora cinnamomi, Fusarium oxysporum, Rhizoctonia solani, and Nectria hematococcus). Ocatin displays substantial amino acid sequence similarity with a widely distributed group of intracellular pathogenesis-related proteins with a hitherto unknown biological function. Our results showed that ocatin serves as a storage protein, has antimicrobial properties, and belongs to the Betv 1/PR-10/MLP protein family. Our findings suggest that an ancient scaffolding protein was recruited in the oca tuber to serve a storage function and that proteins from the Betv 1/PR-10/MLP family might play a role in natural resistance to pathogens.

  14. Ocatin. A Novel Tuber Storage Protein from the Andean Tuber Crop Oca with Antibacterial and Antifungal Activities1

    Science.gov (United States)

    Flores, Teresita; Alape-Girón, Alberto; Flores-Díaz, Marietta; Flores, Hector E.

    2002-01-01

    The most abundant soluble tuber protein from the Andean crop oca (Oxalis tuberosa Mol.), named ocatin, has been purified and characterized. Ocatin accounts for 40% to 60% of the total soluble oca tuber proteins, has an apparent molecular mass of 18 kD and an isoelectric point of 4.8. This protein appears to be found only in tubers and is accumulated only within the cells of the pith and peridermis layers (peel) of the tuber as it develops. Ocatin inhibits the growth of several phytopathogenic bacteria (Agrobacterium tumefaciens, Agrobacterium radiobacter, Serratia marcescens, and Pseudomonas aureofaciens) and fungi (Phytophthora cinnamomi, Fusarium oxysporum, Rhizoctonia solani, and Nectria hematococcus). Ocatin displays substantial amino acid sequence similarity with a widely distributed group of intracellular pathogenesis-related proteins with a hitherto unknown biological function. Our results showed that ocatin serves as a storage protein, has antimicrobial properties, and belongs to the Betv 1/PR-10/MLP protein family. Our findings suggest that an ancient scaffolding protein was recruited in the oca tuber to serve a storage function and that proteins from the Betv 1/PR-10/MLP family might play a role in natural resistance to pathogens. PMID:11950978

  15. Betalains in red and yellow varieties of the Andean tuber crop ulluco (Ullucus tuberosus).

    Science.gov (United States)

    Svenson, Johan; Smallfield, Bruce M; Joyce, Nigel I; Sansom, Catherine E; Perry, Nigel B

    2008-09-10

    The betalain pigments in ulluco (Ullucus tuberosus), a tuberous crop native to the Andes, have been investigated for the first time using LC-DAD-ESI-MS-MS(2) analyses. Five red, yellow, and red-spotted accessions introduced into New Zealand as a new food crop plus two red tetraploid lines were investigated. Thirty-two different betalains were identified. Both the yellow and red tubers were rich in yellow betaxanthins, and the most prominent among the 20 identified were histidine-betaxanthin, arginine-betaxanthin and glutamine-betaxanthin. Arginine-betaxanthin has been reported to occur naturally only once before and was found in yellow ulluco but not in the red tubers. Twelve betacyanins were found in red tubers, with roughly 50% of this content being betanin/isobetanin. Betacyanin levels were up to 70 microg/g fresh weight in red tubers, but were below quantifiable levels in yellow tubers. Betaxanthin levels were up to 50 microg/g fresh weight in yellow tubers. Interference by betacyanins in measuring levels of betaxanthins by visible spectrophotometry is discussed. Low concentrations of betalains were detected in leaves, whereas stems contained total levels similar to the tubers, with dopamine-betaxanthin and betanin being the major pigments. This is the first report describing both the betacyanin and betaxanthin patterns in a plant from the Basellaceae family.

  16. Can quinoa, a salt-tolerant Andean crop species, be used for phytoremediation of chromium-polluted soil?

    Science.gov (United States)

    Ruiz, Karina B.; Cicatelli, Angela; Guarino, Francesco; Jacobsen, Sven-Erik; Biondi, Stefania; Castiglione, Stefano

    2017-04-01

    Quinoa (Chenopodium quinoa Willd), an ancient Andean halophytic seed crop, exhibits exceptional resistance to salinity, drought, and cold. Consistent with the notion that such a resilient plant is likely to tolerate toxic levels of heavy metals as well and could, therefore, be employed for the clean-up of polluted soil (via phytoextraction or phytostabilization), the species' ability to take up, translocate, and tolerate chromium (CrIII) was investigated in a greenhouse pot experiment. A cultivar adapted to European conditions (cv. Titicaca) was grown on soil spiked with 500 mg kg-1 DW of Cr(NO3)3•9H2O, combined (or not) with 150 mM NaCl, or on soil grown with 150 mM NaCl alone. Plants were grown up to maturity (four months after sowing), and then plant biomass and concentrations of Na, Cr, and other elements (e.g., Fe and P) were evaluated in the plant organs. Soil Cr content (total and available fractions) was analysed at the start of the experiment, one week after the last addition of Cr and/or NaCl, and at the end of the trial. No visible toxic effects were observed under the different culture conditions. Results revealed that Cr was mainly accumulated in roots, while Na+ was translocated to the aerial parts. In order to compare plant stress responses under the different treatments (Cr, NaCl, Cr+NaCl), expression levels of several stress-related genes, together with those of a potential Cr transporter, were determined by quantitative real-time RT-PCR.

  17. Oxidative stress and antioxidant responses to increasing concentrations of trivalent chromium in the Andean crop species Chenopodium quinoa Willd.

    Science.gov (United States)

    Scoccianti, Valeria; Bucchini, Anahi E; Iacobucci, Marta; Ruiz, Karina B; Biondi, Stefania

    2016-11-01

    Quinoa (Chenopodium quinoa Willd), an ancient Andean seed crop, exhibits exceptional nutritional properties and resistance to abiotic stress. The species' tolerance to heavy metals has, however, not yet been investigated nor its ability to take up and translocate chromium (Cr). This study aimed to investigate the metabolic adjustments occurring upon exposure of quinoa to several concentrations (0.01-5mM) of CrCl3. Young hydroponically grown plants were used to evaluate Cr uptake, growth, oxidative stress, and other biochemical parameters three and/or seven days after treatment. Leaves accumulated the lowest amounts of Cr, while roots and stems accumulated the most at low and at high metal concentrations, respectively. Fresh weight and photosynthetic pigments were reduced only by the higher Cr(III) doses. Substantially increased lipid peroxidation, hydrogen peroxide, and proline levels were observed only with 5mM Cr(III). Except for a significant decrease at day 7 with 5mM Cr(III), total polyphenols and flavonoids maintained control levels in Cr(III)-treated plants, whereas antioxidant activity increased in a dose-dependent manner. Maximum polyamine accumulation was observed in 1mM CrCl3-treated plants. Even though α- and γ-tocopherols also showed enhanced levels only with the 1mM concentration, tyrosine aminotransferase (TAT, EC 2.6.1.5) activity increased under Cr(III) treatment in a dose- and time-dependent manner. Taken together, results suggest that polyamines, tocopherols, and TAT activity could contribute to tolerance to 1mM Cr(III), but not to the highest concentration that, instead, generated oxidative stress. Copyright © 2016 Elsevier Inc. All rights reserved.

  18. A new nitrogen index for assessment of nitrogen management practices of Andean Mountain cropping systems of Ecuador

    Science.gov (United States)

    Corn (Zea mays L.) is the most important crop for food security in several regions of Ecuador. Small farmers are using nitrogen (N) fertilizer without technical advice based on soil, crop and climatological data. The scientific literature lacks studies where tools are validated that can be used to q...

  19. [Proteus mirabilis septic arthritis].

    Science.gov (United States)

    Sbiti, Mohammed; Bouhamidi, Bahia; Louzi, Lhoussaine

    2017-01-01

    Acute septic arthritis is rare. It is associated with poor prognosis in terms of mortality and morbidity. We report the case of a 61-year old patient with spontaneous Proteus mirabilis septic arthritis. He suffered from complicated diabetes associated with positive blood cultures and synovial fluid cultures. Patient's evolution was favorable thanks to early diagnosis and initiation of adequate antibiotic therapy. Proteus mirabilis septic arthritis is rare. On that basis we conducted a literature review of cases of Proteus mirabilis pyogenic arthritis to highlight the risk factors, pathogenesis, treatment and evolution of these diseases. Diagnosis is commonly based on microbiological analysis, early articular puncture biopsy is performed before the initiation of antibiotic treatment, direct examination, culture and antibiogram which are useful as guidance for antibiotic therapy. Septic arthritis is a diagnostic and therapeutic emergency; early management of this disease allows total healing without after-effects.

  20. Andean waterways

    DEFF Research Database (Denmark)

    Rasmussen, Mattias Borg

    Andean Waterways explores the politics of natural resource use in the Peruvian Andes in the context of climate change and neoliberal expansion. It does so through careful ethnographic analysis of the constitution of waterways, illustrating how water becomes entangled in a variety of political......, social, and cultural concerns. Set in the highland town of Recuay in Ancash, the book traces the ways in which water affects political and ecological relations as glaciers recede. By looking at the shared waterways of four villages located in the foothills of Cordillera Blanca, it addresses pertinent...

  1. Andean waterways

    DEFF Research Database (Denmark)

    Rasmussen, Mattias Borg

    , social, and cultural concerns. Set in the highland town of Recuay in Ancash, the book traces the ways in which water affects political and ecological relations as glaciers recede. By looking at the shared waterways of four villages located in the foothills of Cordillera Blanca, it addresses pertinent......Andean Waterways explores the politics of natural resource use in the Peruvian Andes in the context of climate change and neoliberal expansion. It does so through careful ethnographic analysis of the constitution of waterways, illustrating how water becomes entangled in a variety of political...... questions concerning water governance and rural lives....

  2. Determination of the Morphology of the Starch Granules and the Optimum Internal Cooking Temperature of Four Andean Crops: Oca (Oxalis tuberosa Molina, Olluco (Ullucus tuberosus Loz, Isaño (Tropaeolum tuberosum Ruiz

    Directory of Open Access Journals (Sweden)

    Bellido-Valencia Omar

    2017-12-01

    Full Text Available Andean grains (i.e. quinoa, amaranth have been increasingly studied in recent times, mainly due to the increase in international consumption. However, Andean tubers other than potatoes have not been so widespread and are mainly studied for their starch, previously extracted. This work studied the morphology of native starch in four of these crops (oca, olluco, isaño and aracacha, during cooking and the evolution of their internal temperature in relation to sensory acceptability. Using scanning electron microscopy, it was determined that the size of crude starch granules was between 9 μm to 38.2 μm for oca, 4.48 to 24.9 μm for olluco, 4.45 to 22.9 μm for isaño, and 5.36 to 23.8 μm for arracacha. Sensorially, it was determined that the optimum cooking temperature for arracacha was 89.1°C, 90.9°C for oca, 91°C for isaño, and 91.4 °C for olluco. All samples had optimal cooking times shorter than potato, with the isaño having the best heat transfer.

  3. Arthrite septique à Proteus mirabilis

    Science.gov (United States)

    Sbiti, Mohammed; Bouhamidi, Bahia; Louzi, Lhoussaine

    2017-01-01

    L'arthrite septique aigue est une pathologie peu fréquente mais grevée d'un pronostic lourd en termes de mortalité et morbidité. Nous rapportons un cas d'arthrite septique à Proteus mirabilis survenue de façon spontanée chez un patient de 61 ansprésentant un diabète compliqué,associée à des hémoculturespositives et des cultures positives du liquide articulaire. L'évolution était favorable grâce au diagnostic précoce et à l'institution d'une antibiothérapie adéquate. L'arthrite septique à Proteus Mirabilis est rare, ce qui nous a incité à revoir dans la littérature des séries d'arthrites à pyogènes incluant Proteus mirabilis portant sur les facteurs de risque, la pathogénie, le traitement et l'évolution de ces pathologies. Le diagnostic est avant tout microbiologique, la ponction articulaire précoce est réalisée avant toute antibiothérapie, l'examen direct, la culture et l'antibiogramme qui va guider le choix d'une antibiothérapie. L'arthrite septique est une urgence diagnostique et thérapeutique, la prise en charge précoce de cette pathologie permet une guérison sans séquelles. PMID:28674590

  4. Pathogenesis of Proteus mirabilis Infection

    Science.gov (United States)

    Armbruster, Chelsie E.; Mobley, Harry L. T.; Pearson, Melanie M.

    2017-01-01

    Proteus mirabilis, a Gram-negative rod-shaped bacterium most noted for its swarming motility and urease activity, frequently causes catheter-associated urinary tract infections (CAUTI) that are often polymicrobial. These infections may be accompanied by urolithiasis, development of bladder or kidney stones due to alkalinization of urine from urease-catalyzed urea hydrolysis. Adherence of the bacterium to epithelial and catheter surfaces is mediated by 17 different fimbriae, most notably MR/P fimbriae. Repressors of motility are often encoded by these fimbrial operons. Motility is mediated by flagella encoded on a single contiguous 54 kb chromosomal sequence. On agar plates, P. mirabilis undergoes a morphological conversion to a filamentous swarmer cell expressing hundreds of flagella. When swarms from different strains meet, a line of demarcation, a “Dienes line”, develops due to the killing action of each strain’s type VI secretion system. During infection, histological damage is caused by cytotoxins including hemolysin and a variety of proteases, some autotransported. The pathogenesis of infection, including assessment of individual genes or global screens for virulence or fitness factors has been assessed in murine models of ascending UTI or CAUTI using both single-species and polymicrobial models. Global gene expression studies carried out in culture and in the murine model have revealed the unique metabolism of this bacterium. Vaccines, using MR/P fimbria and its adhesin, MrpH, have been shown to be efficacious in the murine model. A comprehensive review of factors associated with urinary tract infection is presented, encompassing both historical perspectives and current advances. PMID:29424333

  5. Andean settlers rush for Amazonia.

    Science.gov (United States)

    Serra-vega, J

    1990-01-01

    Governments of Andean countries (Peru, Bolivia, Colombia, Ecuador, and Venezuela) have encouraged migration to the Amazon Basin, which has contributed to its destruction. Population pressure, landlessness, and poverty are the inducements to migrate. Efforts to populate the Amazon forest were begun as early as 1964 in Peru without international notice. By 1980, logging was allowed in Peru, and Brazil considered colonization of the Amazon essential to national sovereignty. By 1986, outside of Lima, Peru, a development project originally funded by the World Bank, the InterAmerican Development Bank, and the US, resulted in conflicts between settlers and Indians, in loggers indiscriminately cutting, and in farmers using slash and burn techniques to clear forests. Elsewhere the Peruvian Amazon, in San Ignacio, the population was growing by 5.5%/year. The jungle road that had been started but never completed, Carretera Marginal, destroyed 5 million hectares of primary forest, and much of the 600,000 hectares of arable land gained by the road suffered from inappropriate farming practices which caused massive erosion and laterization of the soils. Food crop production declined, and production of coca for cocaine increased. Coca crops are controlled by the Shining Path guerrillas, who are trying to overthrow the Peruvian government. Devastation of Ecuador around Lago Agrio continues. In Colombia, east of Bogota, forests have disappeared and hills have eroded and silted up rivers and dams. The Andean piedmont in Bolivia has also been devastated by loggers and by slash and burn farming. Southeastern Bolivian forests have been cleared for soya bean cultivation on poor soils. Social and economic crises propel people into the remaining forests. The solution is to ease foreign debt, transfer appropriate technology at affordable prices, refuse to finance destructive development, and help to educate and train scientific researchers. Family planning services are also urgently needed

  6. DNA repair in Proteus mirabilis. Pt. 6

    International Nuclear Information System (INIS)

    Hofmeister, J.; Koehler, H.; Filippov, V.D.

    1979-01-01

    After UV-exposure R46 adds functions to P. mirabilis comparable to those deficient in umuC and uvm mutants of E. coli P. mirabilis possesses functions homologous to those controlled in E. coli by the recA + and lexA + genes. The significance of plasmid-mediated rescue and mutagenic functions for bacteria which lack the misrepair branch of mutagenesis is discussed. (orig./AJ) 891 AJ/orig.- 892 AMO [de

  7. Spinal osteomyelitis caused by Proteus mirabilis in a child

    NARCIS (Netherlands)

    deWeerd, W; Kimpen, JLL; Miedema, CJ

    Osteomyelitis due to Proteus mirabilis is rare. Spinal osteomyelitis caused by this organism has only been described in adults. This is the first paediatric case of P. mirabilis vertebral osteomyelitis.

  8. DNA repair in proteus mirabilis. 5

    International Nuclear Information System (INIS)

    Hofemeister, J.; Boehme, H.; Fleischhacker, M.; Adler, B.; Eitner, G.; Steinborn, G.

    1979-01-01

    Inducible cell lysis in UV-irradiated cultures of Proteus mirabilis PG VI rec + derivatives is due to the induction of a defective prophage dP VI. This lytic response is found to account for the extraordinary high UV sensitivity of P. mirabilis rec + strains. Lysis of cells after UV or mitomycin C treatment is accompanied by the release of various defective phage particles including head-tail, tail and polysheath structures. Survival of P. mirabilis is shown to be strongly affected by the plating procedure. It is supposed that plating of UV-irradiated rec + cells after a distinct time of growth in liquid medium some how enhances the efficiency of repair activities rather than to prevent inducible cell lysis in P. mirabilis from occurring. The resident defective prophage interferes with the multiplication of infectious temperate phages (π1) as indicated by the efficiency of plating and plaque size. The extent of host-controlled reactivation of UV-irradiated phages, however, was not influenced by the lysogenic state of the host bacteria. The derepression of the resident prophage is thought to correlate with the appearence after UV exposure of a distinct and relatively stable low molecular weight DNA which is assumed to originate from enzymatic fragmentation rather than postmortal nicking of chromosomal DNA in P. mirabilis. Mutants are described which lost the inducible cell lysis response and, in case of PG 1300- also lost the immunity for bacteriolytic activities formed by PG VI strains after lysogenic induction. Whilst this derivative of P. mirabilis might either be cured for the defective prophage or rendered noninducible by mutation this substrain lost also both the extrasensitivity against UV and the chromosomal DNA fragmentation. The extent of the UV-induced DNA degradation response seems not to be markedly affected by these DNA fragmentations. (author)

  9. Proteus mirabilis and Urinary Tract Infections

    Science.gov (United States)

    Schaffer, Jessica N.; Pearson, Melanie M.

    2015-01-01

    Proteus mirabilis is a Gram-negative bacterium which is well-known for its ability to robustly swarm across surfaces in a striking bulls’-eye pattern. Clinically, this organism is most frequently a pathogen of the urinary tract, particularly in patients undergoing long-term catheterization. This review covers P. mirabilis with a focus on urinary tract infections (UTI), including disease models, vaccine development efforts, and clinical perspectives. Flagella-mediated motility, both swimming and swarming, is a central facet of this organism. The regulation of this complex process and its contribution to virulence is discussed, along with the type VI-secretion system-dependent intra-strain competition which occurs during swarming. P. mirabilis uses a diverse set of virulence factors to access and colonize the host urinary tract, including urease and stone formation, fimbriae and other adhesins, iron and zinc acquisition, proteases and toxins, biofilm formation, and regulation of pathogenesis. While significant advances in this field have been made, challenges remain to combatting complicated UTI and deciphering P. mirabilis pathogenesis. PMID:26542036

  10. Characterization of Proteus mirabilis isolates from broilers

    Science.gov (United States)

    Background: Proteus mirabilis is ubiquitous in the environment and is regarded as a part of the normal flora in human gastrointestinal tract. However, this bacterium is also an opportunistic human pathogen that causes urinary tract infections. Recently, this microorganism has been isolated from m...

  11. Proteus mirabilis and Urinary Tract Infections.

    Science.gov (United States)

    Schaffer, Jessica N; Pearson, Melanie M

    2015-10-01

    Proteus mirabilis is a Gram-negative bacterium and is well known for its ability to robustly swarm across surfaces in a striking bulls'-eye pattern. Clinically, this organism is most frequently a pathogen of the urinary tract, particularly in patients undergoing long-term catheterization. This review covers P. mirabilis with a focus on urinary tract infections (UTI), including disease models, vaccine development efforts, and clinical perspectives. Flagella-mediated motility, both swimming and swarming, is a central facet of this organism. The regulation of this complex process and its contribution to virulence is discussed, along with the type VI-secretion system-dependent intra-strain competition, which occurs during swarming. P. mirabilis uses a diverse set of virulence factors to access and colonize the host urinary tract, including urease and stone formation, fimbriae and other adhesins, iron and zinc acquisition, proteases and toxins, biofilm formation, and regulation of pathogenesis. While significant advances in this field have been made, challenges remain to combatting complicated UTI and deciphering P. mirabilis pathogenesis.

  12. Antibiotics profiling of Proteus mirabilis and Pseudomonas ...

    African Journals Online (AJOL)

    Constant tracking of the antibiotic susceptibilities of these organisms at different region within each country is of great epidemiological value to formulate well informed and scientific based preventive measures to curtail the spread of drug resistant pathogens through the food chain. We screened 19 Proteus mirabilis and 35 ...

  13. Andean region study

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1999-10-01

    New opportunities for climate change mitigation arising from a higher energy integration among Andean Pact nations were analysed within the framework of the UNEP/GEF Project. Apart from the search for regional mitigation actions, the study was mainly aimed at detecting methodological problems which arise when passing from a strictly national view to the co-ordination of regional actions to deal with climate change. In accordance with the available resources and data, and in view of the mainly methodological nature of the project, it was decided to analyse the opportunities to delve into the energy integration of the Region as regards electricity and natural gas industries and their eventual impact on the emission of greenhouse gases. Although possibilities of setting up electricity and natural gas markets are real, their impacts on GHG emission from the energy system would not prove substantially higher than those which the nations could achieve through the use of their own energy resources, in view that the Andean systems are competitive rather than complementary. More in-depth studies and detail information will be required - unavailable for the present study - to be able to properly evaluate all benefits associated with higher energy integration. Nevertheless, the supply of natural gas to Ecuador seems to be the alternative with the highest impact on GHG emission. If we were to analyse the supply and final consumption of energy jointly, we would most certainly detect additional mitigation options resulting from higher co-operation and co-ordination in the energy field. (EHS)

  14. Proteus mirabilis and its role in dacryocystitis.

    Science.gov (United States)

    Borgman, Christopher J

    2014-09-01

    Dacryocystitis is a common inflammation of the lacrimal sac, usually from an underlying bacterial infection. Most cases can be attributed to common ocular flora such as Staphylococcus aureus or Streptococcus pneumoniae; however, uncommon bacterial causes such as Proteus mirabilis can still occur. P. mirabilis is a gram-negative bacillus that is found abundantly in people who have undergone long-term catheterization and is uncommonly found in or around the eyes except in people who have undergone long-term catheterization. Proteus species can cause conjunctivitis, canaliculitis, and dacryocystitis and have the ability to manifest into preseptal cellulitis; therefore, timely recognition and treatment are important to prevent potential further complications. An 84-year-old white man had several recurrences of acute dacryocystitis that had marginal improvement with empiric antibiotic treatment. Resultant culture of the ocular discharge revealed an uncommon bacterium, P. mirabilis. Successful dosing of oral antibiotics resolved the infection but ultimately the patient required an external dacryocystorhinostomy (DCR) procedure to maintain patency of the nasolacrimal lacrimal system and prevent recurrence. At 3 months after external DCR, the patient was symptom free with no recurrences. Dacryocystitis has a distinctive clinical presentation and is usually easily treated when appropriate oral antibiotics are directed at the underlying pathogen. In nonresponsive cases, culturing of the ocular discharge should be performed to identify the underlying pathogen. Cases of dacryocystitis caused by Proteus species are usually responsive to several standard antibiotics used orally in eye care; however, culturing and susceptibility testing can streamline the diagnostic and management sequence considerably in unclear or unresponsive cases. Typically, patients with dacryocystitis return to normal after appropriate treatment, but chronic recurrences and epiphora are potential sequelae

  15. Collective motion in Proteus mirabilis swarms

    Science.gov (United States)

    Haoran, Xu

    Proteus mirabilisis a Gram-negative, rod-shaped bacterium. It is widely distributed in soil and water, and it is well known for exhibiting swarming motility on nutrient agar surfaces. In our study, we focused on the collective motility of P. mirabilis and uncovered a range of interesting phenomena. Here we will present our efforts to understand these phenomena through experiments and simulation. Mailing address: Room 306 Science Centre North Block, The Chinese University of Hong Kong, Shatin, N.T. Hong Kong SAR. Phone: +852-3943-6354. Fax: +852-2603-5204. E-mail:xhrphx@gmail.com.

  16. Hemoglobin affinity in Andean rodents

    Directory of Open Access Journals (Sweden)

    HRVOJ OSTOJIC

    2002-01-01

    Full Text Available Blood hemoglobin oxygen affinity (P50 was measured in three Andean species and in the laboratory rat (control, all raised near sea level. Chinchilla lanigera (Molina, 1792 has an altitudinal habitat range from low Andean slopes up to 3000 m., while Chinchilla brevicaudata (Waterhouse, 1848 has an altitudinal range from 3000 to 5000 m. The laboratory type guinea pig, wild type guinea pig (Cavia porcellus, (Waterhouse, 1748, and laboratory rat (Rattus norvegicus were also raised at sea level. The Andean species had high hemoglobin oxygen affinities (low P50 compared with the rat. Chinchilla brevicaudata had a higher affinity than Chinchilla lanigera. The wild type guinea pig had a higher affinity than the laboratory type. As has been shown in other species, this is another example of an inverse correlation between the altitude level and the P50 values. This is the first hemoglobin oxygen affinity study in Chinchilla brevicaudata.

  17. Mathematical Modeling the Geometric Regularity in Proteus Mirabilis Colonies

    Science.gov (United States)

    Zhang, Bin; Jiang, Yi; Minsu Kim Collaboration

    Proteus Mirabilis colony exhibits striking spatiotemporal regularity, with concentric ring patterns with alternative high and low bacteria density in space, and periodicity for repetition process of growth and swarm in time. We present a simple mathematical model to explain the spatiotemporal regularity of P. Mirabilis colonies. We study a one-dimensional system. Using a reaction-diffusion model with thresholds in cell density and nutrient concentration, we recreated periodic growth and spread patterns, suggesting that the nutrient constraint and cell density regulation might be sufficient to explain the spatiotemporal periodicity in P. Mirabilis colonies. We further verify this result using a cell based model.

  18. 1932: ''annus mirabilis'' for nuclear physics

    International Nuclear Information System (INIS)

    Hughes, J.

    1998-01-01

    1932 was an extraordinary year for nuclear physics: J. Chadwick discovered the neutron, C.D.Anderson identified the positron and the first artificial disintegration was realised with a particle accelerator by J.Cockcroft and E.Walton. These 3 discoveries transformed nuclear physics by providing basis on which any new research could be led. The neutron allowed the discovery of artificial radioactivity by Joliot-Curie in 1934 and later the discovery of nuclear fission by O. Hahn, F. Strassman and L. Meitner. The positron brought new concepts about cosmic radiation and drew the way to the discovery of new particles. Artificial disintegration paved the way to the ever-bigger machines. It was the beginning of the era of breaking nuclei. 1932 deserves its title of ''annus mirabilis'' of physics. This article presents the quick evolution of ideas, concepts in nuclear physics in the thirties. (A.C.)

  19. DNA repair in proteus mirabilis. Pt. 4

    International Nuclear Information System (INIS)

    Hofemeister, J.

    1977-01-01

    Post-irradiation DNA degradation in P. mirabilis rec + strains after UV irradiation is found to be more extensive in starvation buffer than in growth medium. In growth medium restriction of protein synthesis, but not DNA synthesis, largely prevents the expression of 'breakdown limitation'. By the addition of chloramphenicol during post-irradiation incubation in growth medium the expression of breakdown limitation was followed and found to occur 20 to 40 min after UV irradiation. Pre-irradiation by a low dose of UV leads after a corresponding time of post-irradiation incubation to breakdown limitation even in starvation buffer after a second UV exposure. Post-irradiation DNA degradation is presumed to be initiated at the sites of DNA lesions which arise at replication points damaged by UV. While pre-starvation restricts the efficiency of postirradiation DNA degradation by the reduction of the number of replication points active at the time of irradiation, caffeine as well as 2.4-dinitrophenol inhibit DNA degradation even in rec - cells probably by the interference with nicking or exonucleoltytic events initiated at those sites in the absence of breakdown limitation. Breakdown limitation is postulated to be due to inducible derepression of REC-functions which lead to the protection and, probably, repair of DNA lesions arising at the replication points following UV exposure. (orig.) [de

  20. [Etiological and molecular characteristics of diarrhea caused Proteus mirabilis].

    Science.gov (United States)

    Shi, Xiaolu; Hu, Qinghua; Lin, Yiman; Qiu, Yaqun; Li, Yinghui; Jiang, Min; Chen, Qiongcheng

    2014-06-01

    To analyze the etiological characteristics, virulence genes and plasmids that carrying diarrhea-causing Proteus mirabilis and to assess their relationship with drug resistance and pathogenicity. Proteus mirabilis coming from six different sources (food poisoning, external environment and healthy people) were analyzed biochemically, on related susceptibility and pulsed-field gel electrophoresis (PFGE). Virulence genes were detected by PCR. Plasmids were extracted and sequenced after gel electrophoresis purification. The biochemical characteristics of Proteus mirabilis from different sources seemed basically the same, and each of them showed having common virulence genes, as ureC, rsmA, hpmA and zapA. However, the PFGE patterns and susceptibility of these strains were different, so as the plasmids that they carried. Plasmid that presented in the sequenced strain showed that the 2 683 bp length plasmid encodes qnrD gene was associated with the quinolone resistance. Etiological characteristics and molecular characteristics of Proteus mirabilis gathered from different sources, were analyzed. Results indicated that traditional biochemical analysis and common virulence gene identification might be able to distinguish the strains with different sources. However, PFGE and plasmids analysis could distinguish the sources of strains and to identify those plasmids that commonly carried by the drug-resistant strains. These findings also provided theoretical basis for further study on the nature of resistance and pathogenicity in Proteus mirabilis.

  1. Merging mythology and morphology: the multifaceted lifestyle of Proteus mirabilis.

    Science.gov (United States)

    Armbruster, Chelsie E; Mobley, Harry L T

    2012-11-01

    Proteus mirabilis, named for the Greek god who changed shape to avoid capture, has fascinated microbiologists for more than a century with its unique swarming differentiation, Dienes line formation and potent urease activity. Transcriptome profiling during both host infection and swarming motility, coupled with the availability of the complete genome sequence for P. mirabilis, has revealed the occurrence of interbacterial competition and killing through a type VI secretion system, and the reciprocal regulation of adhesion and motility, as well as the intimate connections between metabolism, swarming and virulence. This Review addresses some of the unique and recently described aspects of P. mirabilis biology and pathogenesis, and emphasizes the potential role of this bacterium in single-species and polymicrobial urinary tract infections.

  2. Merging mythology and morphology: the multifaceted lifestyle of Proteus mirabilis

    Science.gov (United States)

    Armbruster, Chelsie E.; Mobley, Harry L. T.

    2013-01-01

    Proteus mirabilis, named for the Greek god who changed shape to avoid capture, has fascinated microbiologists for more than a century with its unique swarming differentiation, Dienes line formation and potent urease activity. Transcriptome profiling during both host infection and swarming motility, coupled with the availability of the complete genome sequence for P. mirabilis, has revealed the occurrence of interbacterial competition and killing through a type VI secretion system, and the reciprocal regulation of adhesion and motility, as well as the intimate connections between metabolism, swarming and virulence. This Review addresses some of the unique and recently described aspects of P. mirabilis biology and pathogenesis, and emphasizes the potential role of this bacterium in single- species and polymicrobial urinary tract infections. PMID:23042564

  3. Comparative Screening of Digestion Tract Toxic Genes in Proteus mirabilis.

    Science.gov (United States)

    Shi, Xiaolu; Lin, Yiman; Qiu, Yaqun; Li, Yinghui; Jiang, Min; Chen, Qiongcheng; Jiang, Yixiang; Yuan, Jianhui; Cao, Hong; Hu, Qinghua; Huang, Shenghe

    2016-01-01

    Proteus mirabilis is a common urinary tract pathogen, and may induce various inflammation symptoms. Its notorious ability to resist multiple antibiotics and to form urinary tract stones makes its treatment a long and painful process, which is further challenged by the frequent horizontal gene transferring events in P. mirabilis genomes. Three strains of P. mirabilis C02011/C04010/C04013 were isolated from a local outbreak of a food poisoning event in Shenzhen, China. Our hypothesis is that new genes may have been acquired horizontally to exert the digestion tract infection and toxicity. The functional characterization of these three genomes shows that each of them independently acquired dozens of virulent genes horizontally from the other microbial genomes. The representative strain C02011 induces the symptoms of both vomit and diarrhea, and has recently acquired a complete type IV secretion system and digestion tract toxic genes from the other bacteria.

  4. Exploring the potential of an Andean fruit

    NARCIS (Netherlands)

    Olivares Tenorio, Mary Luz

    2017-01-01

    Cape gooseberry is a fruit cultivated in Andean countries. Currently it is available some international markets, besides the domestic Andean market. Colombia is the major producer and export country at the moment. The value chain of cape gooseberry faces several barriers of technological and

  5. Beet western yellows virus infects the carnivorous plant Nepenthes mirabilis.

    Science.gov (United States)

    Miguel, Sissi; Biteau, Flore; Mignard, Benoit; Marais, Armelle; Candresse, Thierry; Theil, Sébastien; Bourgaud, Frédéric; Hehn, Alain

    2016-08-01

    Although poleroviruses are known to infect a broad range of higher plants, carnivorous plants have not yet been reported as hosts. Here, we describe the first polerovirus naturally infecting the pitcher plant Nepenthes mirabilis. The virus was identified through bioinformatic analysis of NGS transcriptome data. The complete viral genome sequence was assembled from overlapping PCR fragments and shown to share 91.1 % nucleotide sequence identity with the US isolate of beet western yellows virus (BWYV). Further analysis of other N. mirabilis plants revealed the presence of additional BWYV isolates differing by several insertion/deletion mutations in ORF5.

  6. The Dienes phenomenon: competition and territoriality in Swarming Proteus mirabilis

    NARCIS (Netherlands)

    Budding, A. E.; Ingham, C. J.; Bitter, W.; Vandenbroucke-Grauls, C. M.; Schneeberger, P. M.

    2009-01-01

    When two different strains of swarming Proteus mirabilis encounter one another on an agar plate, swarming ceases and a visible line of demarcation forms. This boundary region is known as the Dienes line and is associated with the formation of rounded cells. While the Dienes line appears to be the

  7. Urinary Tract Infection Caused by a Capnophilic Proteus mirabilis Strain

    NARCIS (Netherlands)

    Trapman, M.; Ingen, J. van; Keijman, J.; Swanink, C.M.A.

    2015-01-01

    From a urine sample from a patient with a urinary tract infection, a carbon dioxide-dependent Proteus mirabilis strain was isolated. It is important to perform urine cultures in 5% carbon dioxide and an anaerobic atmosphere if bacteria prominent in Gram stains do not grow on routine media in ambient

  8. Proteus mirabilis interkingdom swarming signals attract blow flies

    Science.gov (United States)

    Flies transport specific bacteria with their larvae which provides a wider range of nutrients for those bacteria. Our hypothesis was that this symbiotic interaction may depend on interkingdom signaling. We obtained Proteus mirabilis from the salivary glands of the blow fly Lucilia sericat. This s...

  9. Fimbriae have distinguishable roles in Proteus mirabilis biofilm formation.

    Science.gov (United States)

    Scavone, Paola; Iribarnegaray, Victoria; Caetano, Ana Laura; Schlapp, Geraldine; Härtel, Steffen; Zunino, Pablo

    2016-07-01

    Proteus mirabilis is one of the most common etiological agents of complicated urinary tract infections, especially those associated with catheterization. This is related to the ability of P. mirabilis to form biofilms on different surfaces. This pathogen encodes 17 putative fimbrial operons, the highest number found in any sequenced bacterial species so far. The present study analyzed the role of four P. mirabilis fimbriae (MR/P, UCA, ATF and PMF) in biofilm formation using isogenic mutants. Experimental approaches included migration over catheter, swimming and swarming motility, the semiquantitative assay based on adhesion and crystal violet staining, and biofilm development by immunofluorescence and confocal microscopy. Different assays were performed using LB or artificial urine. Results indicated that the different fimbriae contribute to the formation of a stable and functional biofilm. Fimbriae revealed particular associated roles. First, all the mutants showed a significantly reduced ability to migrate across urinary catheter sections but neither swimming nor swarming motility were affected. However, some mutants formed smaller biofilms compared with the wild type (MRP and ATF) while others formed significantly larger biofilms (UCA and PMF) showing different bioarchitecture features. It can be concluded that P. mirabilis fimbriae have distinguishable roles in the generation of biofilms, particularly in association with catheters. © FEMS 2016. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  10. Andean rural children's views of the environment: A qualitative study

    Science.gov (United States)

    Maurial, Mahia

    Andean rural children's drawings and narratives about their crops and the immediate biological environment are rich tools to understand local views of the environment. Children's drawings and narratives were collected and linked to interviews as well as participant observation gathered from parents, leaders and teachers. The research sites are the community of Willca and the school of Mayu. Fieldwork was completed in 1998. In the conceptual framework I distinguish between two dissimilar knowledges, school knowledge and local knowledge. These knowledges produce two dissimilar views of the environment. I further analyze relationships of knowledge and power and argue that school knowledge overpowers local knowledge. Concomitantly, I studied set of ideas associated with two knowledges aforementioned: superacion (surpass) and regeneration (Apffel-Marglin 1995). Although these ideas coexist in peoples' minds they are not linked or effectively connected. In order to link local knowledge and school knowledge together, I propose the integration of environmental studies and art education to enhance a local sense of place (Blandy et. al 1993) in Andean and other schools. This will contribute to grassroots educational policy.

  11. Flagellum Density Regulates Proteus mirabilis Swarmer Cell Motility in Viscous Environments

    OpenAIRE

    Tuson, Hannah H.; Copeland, Matthew F.; Carey, Sonia; Sacotte, Ryan; Weibel, Douglas B.

    2013-01-01

    Proteus mirabilis is an opportunistic pathogen that is frequently associated with urinary tract infections. In the lab, P. mirabilis cells become long and multinucleate and increase their number of flagella as they colonize agar surfaces during swarming. Swarming has been implicated in pathogenesis; however, it is unclear how energetically costly changes in P. mirabilis cell morphology translate into an advantage for adapting to environmental changes. We investigated two morphological changes...

  12. Detection of bla KPC-2 in Proteus mirabilis in Brazil

    Directory of Open Access Journals (Sweden)

    Adriane Borges Cabral

    2015-02-01

    Full Text Available INTRODUCTION : Infections caused by Klebsiella pneumoniae carbapenemase (KPC-producing isolates pose a major worldwide public health problem today. METHODS : A carbapenem-resistant Proteus mirabilis clinical isolate was investigated for plasmid profiles and the occurrence of β-lactamase genes. RESULTS : The isolate exhibited resistance to ertapenem and imipenem and was susceptible to meropenem, polymyxin, and tigecycline. Five plasmids were identified in this isolate. DNA sequencing analysis revealed the presence of bla KPC-2 and bla TEM-1 genes. An additional PCR using plasmid DNA confirmed that bla KPC-2 was present in one of these plasmids. Conclusions: We report the detection of bla KPC-2 in P. mirabilis in Brazil for the first time. This finding highlights the continuous transfer of bla KPC between bacterial genera, which presents a serious challenge to the prevention of infection by multidrug-resistant bacteria.

  13. Neonatal Meningoventriculitis Due to Proteus Mirabilis – A Case Report

    Science.gov (United States)

    Juyal, Deepak; Rathaur, Vyas Kumar; Sharma, Neelam

    2013-01-01

    A five day old full term born baby was admitted to our Neonatal Intensive Care Unit with seizures, opisthotonous posture and was icteric upto thigh. Baby had a three day history of poor feeding, lethargy and abnormal body movements. Mother was a 29 years old primigravida and had a normal vaginal delivery at home. Sepsis profile of the patient was requested, lumbar puncture and ventricular tap was performed. Patient was put on third generation cephalosporins, aminoglycosides and phenobarbitone. Culture and sensitivity report of blood, Cerebro spinal fluid and ventricular fluid showed Proteus mirabilis. Computerized Tomography scan showed a large parenchymal lesion in the right frontal lobe and diffuse ependymal enhancement along both the lateral ventricles suggestive of meningoventriculitis. We hereby present a fatal case of neonatal meningoventriculitis due to Proteus mirabilis. PMID:23543669

  14. New aspects of RpoE in uropathogenic Proteus mirabilis.

    Science.gov (United States)

    Liu, Ming-Che; Kuo, Kuan-Ting; Chien, Hsiung-Fei; Tsai, Yi-Lin; Liaw, Shwu-Jen

    2015-03-01

    Proteus mirabilis is a common human pathogen causing recurrent or persistent urinary tract infections (UTIs). The underlying mechanisms for P. mirabilis to establish UTIs are not fully elucidated. In this study, we showed that loss of the sigma factor E (RpoE), mediating extracytoplasmic stress responses, decreased fimbria expression, survival in macrophages, cell invasion, and colonization in mice but increased the interleukin-8 (IL-8) expression of urothelial cells and swarming motility. This is the first study to demonstrate that RpoE modulated expression of MR/P fimbriae by regulating mrpI, a gene encoding a recombinase controlling the orientation of MR/P fimbria promoter. By real-time reverse transcription-PCR, we found that the IL-8 mRNA amount of urothelial cells was induced significantly by lipopolysaccharides extracted from rpoE mutant but not from the wild type. These RpoE-associated virulence factors should be coordinately expressed to enhance the fitness of P. mirabilis in the host, including the avoidance of immune attacks. Accordingly, rpoE mutant-infected mice displayed more immune cell infiltration in bladders and kidneys during early stages of infection, and the rpoE mutant had a dramatically impaired ability of colonization. Moreover, it is noteworthy that urea (the major component in urine) and polymyxin B (a cationic antimicrobial peptide) can induce expression of rpoE by the reporter assay, suggesting that RpoE might be activated in the urinary tract. Altogether, our results indicate that RpoE is important in sensing environmental cues of the urinary tract and subsequently triggering the expression of virulence factors, which are associated with the fitness of P. mirabilis, to build up a UTI. Copyright © 2015, American Society for Microbiology. All Rights Reserved.

  15. Wound healing activity of Ullucus tuberosus, an Andean tuber crop

    OpenAIRE

    Nathalie Heil; Karent Bravo; Andrés Montoya; Sara Robledo; Edison Osorio

    2017-01-01

    Objective: This study was designed to investigate the wound healing activity of aqueous extracts of Ullucus tuberosus (U. tuberosus) using in vitro models. Methods: Lyophilized pulp and acetone extracts of U. tuberosus were produced using ultrasound extraction. The capacity for collagenase activation was evaluated using fluorescence detection of the enzymatic activity. Then, the influence of U. tuberosus extracts on cell proliferation, cell migration and synthesis of the extracellular matr...

  16. Bioactive Potential of Andean Fruits, Seeds, and Tubers.

    Science.gov (United States)

    Campos, David; Chirinos, Rosana; Gálvez Ranilla, Lena; Pedreschi, Romina

    2018-01-01

    The Andes is considered the longest continental mountain range in the world. It covers 7000km long and about 200-700km wide and an average height of about 4000m. Very unique plant species are endemic of this area including fruits (e.g., lucuma, cherimoya, sweet pepino, sauco), roots and tubers (potatoes, sweet potatoes, yacón, chicuru, mashua, olluco, etc.), and seeds (quinoa, amaranth, tarwi, etc.). These crops have been used for centuries by the native population and relatively recently have gained the world attention due to the wide range of nutrients and/or phytochemicals they possess. In this chapter, main Andean fruits, seeds, and roots and tubers have been selected and detailed nutritional and functional information is provided. In addition, traditional and current uses are provided and their bioactive potential is reported based on published scientific literature. © 2018 Elsevier Inc. All rights reserved.

  17. Genetic diversity of Phytophthora infestans in the Northern Andean region.

    Science.gov (United States)

    Cárdenas, Martha; Grajales, Alejandro; Sierra, Roberto; Rojas, Alejandro; González-Almario, Adriana; Vargas, Angela; Marín, Mauricio; Fermín, Gustavo; Lagos, Luz E; Grünwald, Niklaus J; Bernal, Adriana; Salazar, Camilo; Restrepo, Silvia

    2011-02-09

    Phytophthora infestans (Mont.) de Bary, the causal agent of potato late blight, is responsible for tremendous crop losses worldwide. Countries in the northern part of the Andes dedicate a large proportion of the highlands to the production of potato, and more recently, solanaceous fruits such as cape gooseberry (Physalis peruviana) and tree tomato (Solanum betaceum), all of which are hosts of this oomycete. In the Andean region, P. infestans populations have been well characterized in Ecuador and Peru, but are poorly understood in Colombia and Venezuela. To understand the P. infestans population structure in the Northern part of the Andes, four nuclear regions (ITS, Ras, β-tubulin and Avr3a) and one mitochondrial (Cox1) region were analyzed in isolates of P. infestans sampled from different hosts in Colombia and Venezuela. Low genetic diversity was found within this sample of P. infestans isolates from crops within several regions of Colombia and Venezuela, revealing the presence of clonal populations of the pathogen in this region. We detected low frequency heterozygotes, and their distribution patterns might be a consequence of a high migration rate among populations with poor effective gene flow. Consistent genetic differentiation exists among isolates from different regions. The results here suggest that in the Northern Andean region P. infestans is a clonal population with some within-clone variation. P. infestans populations in Venezuela reflect historic isolation that is being reinforced by a recent self-sufficiency of potato seeds. In summary, the P. infestans population is mainly shaped by migration and probably by the appearance of variants of key effectors such as Avr3a.

  18. Genetic diversity of Phytophthora infestans in the Northern Andean region

    Directory of Open Access Journals (Sweden)

    Grünwald Niklaus J

    2011-02-01

    Full Text Available Abstract Background Phytophthora infestans (Mont. de Bary, the causal agent of potato late blight, is responsible for tremendous crop losses worldwide. Countries in the northern part of the Andes dedicate a large proportion of the highlands to the production of potato, and more recently, solanaceous fruits such as cape gooseberry (Physalis peruviana and tree tomato (Solanum betaceum, all of which are hosts of this oomycete. In the Andean region, P. infestans populations have been well characterized in Ecuador and Peru, but are poorly understood in Colombia and Venezuela. To understand the P. infestans population structure in the Northern part of the Andes, four nuclear regions (ITS, Ras, β-tubulin and Avr3a and one mitochondrial (Cox1 region were analyzed in isolates of P. infestans sampled from different hosts in Colombia and Venezuela. Results Low genetic diversity was found within this sample of P. infestans isolates from crops within several regions of Colombia and Venezuela, revealing the presence of clonal populations of the pathogen in this region. We detected low frequency heterozygotes, and their distribution patterns might be a consequence of a high migration rate among populations with poor effective gene flow. Consistent genetic differentiation exists among isolates from different regions. Conclusions The results here suggest that in the Northern Andean region P. infestans is a clonal population with some within-clone variation. P. infestans populations in Venezuela reflect historic isolation that is being reinforced by a recent self-sufficiency of potato seeds. In summary, the P. infestans population is mainly shaped by migration and probably by the appearance of variants of key effectors such as Avr3a.

  19. Two new Cystoderma species from high Andean Ecuador

    DEFF Research Database (Denmark)

    Saar, I.; Læssøe, Thomas

    2006-01-01

    ABSTRACT: Two new agaric species, Cystoderma andinum and C. papallactae are described from high Andean Ecuador.......ABSTRACT: Two new agaric species, Cystoderma andinum and C. papallactae are described from high Andean Ecuador....

  20. Comparison of antibiotic resistance patterns in collections of Escherichia coli and Proteus mirabilis uropathogenic strains.

    Science.gov (United States)

    Adamus-Bialek, Wioletta; Zajac, Elzbieta; Parniewski, Pawel; Kaca, Wieslaw

    2013-04-01

    Escherichia coli and Proteus mirabilis are important urinary tract pathogens. The constant increase in the antibiotic resistance of clinical bacterial strains has become an important clinical problem. The aim of this study was to compare the antibiotic resistance of 141 clinical (Sweden and Poland) and 42 laboratory (Czech Republic) P. mirabilis strains and 129 clinical (Poland) uropathogenic E. coli strains. The proportion of unique versus diverse patterns in Swedish clinical and laboratory P. mirabilis strain collections was comparable. Notably, a similar proportion of unique versus diverse patterns was observed in Polish clinical P. mirabilis and E. coli strain collections. Mathematical models of the antibiotic resistance of E. coli and P. mirabilis strains based on Kohonen networks and association analysis are presented. In contrast to the three clinical strain collections, which revealed complex associations with the antibiotics tested, laboratory P. mirabilis strains provided simple antibiotic association diagrams. The monitoring of antibiotic resistance patterns of clinical E. coli and P. mirabilis strains plays an important role in the treatment procedures for urinary tract infections and is important in the context of the spreading drug resistance in uropathogenic strain populations. The adaptability and flexibility of the genomes of E. coli and P. mirabilis strains are discussed.

  1. In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilis.

    Science.gov (United States)

    Savory, Nasa; Lednor, Danielle; Tsukakoshi, Kaori; Abe, Koichi; Yoshida, Wataru; Ferri, Stefano; Jones, Brian V; Ikebukuro, Kazunori

    2013-10-01

    Proteus mirabilis is a prominent cause of catheter-associated urinary tract infections (CAUTIs) among patients undergoing long-term bladder catheterization. There are currently no effective means of preventing P. mirabilis infections, and strategies for prophylaxis and rapid early diagnosis are urgently required. Aptamers offer significant potential for development of countermeasures against P. mirabilis CAUTI and are an ideal class of molecules for the development of diagnostics and therapeutics. Here we demonstrate the application of Cell-SELEX to identify DNA aptamers that show high affinity for P. mirabilis. While the aptamers identified displayed high affinity for P. mirabilis cells in dot blotting assays, they also bound to other uropathogenic bacteria. To improve aptamer specificity for P. mirabilis, an in silico maturation (ISM) approach was employed. Two cycles of ISM allowed the identification of an aptamer showing 36% higher specificity, evaluated as a ratio of binding signal for P. mirabilis to that for Escherichia coli (also a cause of CAUTI and the most common urinary tract pathogen). Aptamers that specifically recognize P. mirabilis would have diagnostic and therapeutic values and constitute useful tools for studying membrane-associated proteins in this organism. Copyright © 2013 Wiley Periodicals, Inc.

  2. Forms of memory in Andean queros

    Directory of Open Access Journals (Sweden)

    Alfredo Cordiviola

    2017-12-01

    Full Text Available In colonial times, queros were used to drink chicha and to formalize pacts in ritual ceremonies by the indigenous Andean elites. They had been manufactured for centuries, long before the Incas dominated the region, and continued to be made after Spanish domination and compulsory evangelization had been established. During the Inca empire, the surface of the queros was covered just with incisions and geometric forms; in the viceroyalty of Peru they were covered instead with figurative images. In this work we analyze the evolution of these forms and the different ways in which these ceremonial vessels were used to evoke the Andean pasts.

  3. Observation of multicellular spinning behavior of Proteus mirabilis by atomic force microscopy and multifunctional microscopy.

    Science.gov (United States)

    Liu, Yanxia; Deng, Yuanxin; Luo, Shuxiu; Deng, Yu; Guo, Linming; Xu, Weiwei; Liu, Lei; Liu, Junkang

    2014-01-01

    This study aimed to observe the multicellular spinning behavior of Proteus mirabilis by atomic force microscopy (AFM) and multifunctional microscopy in order to understand the mechanism underlying this spinning movement and its biological significance. Multifunctional microscopy with charge-coupled device (CCD) and real-time AFM showed changes in cell structure and shape of P. mirabilis during multicellular spinning movement. Specifically, the morphological characteristics of P. mirabilis, multicellular spinning dynamics, and unique movement were observed. Our findings indicate that the multicellular spinning behavior of P. mirabilis may be used to collect nutrients, perform colonization, and squeeze out competitors. The movement characteristics of P. mirabilis are vital to the organism's biological adaptability to the surrounding environment. Copyright © 2013 Elsevier Ltd. All rights reserved.

  4. Administrative Law in the Andean Community of Nations

    Directory of Open Access Journals (Sweden)

    Jorge Enrique Santos Rodríguez

    2013-12-01

    Full Text Available One of the contemporary tendencies of Administrative Law is the recognition of its existence beyond the borders of a State. Under such premise, this paper aims to demonstrate that in the Andean Community of Nations sufficient elements to consider the existence of an Andean administrative Law. In the Andean statutes and rules, it is possible to identify an administrative function, as well as an administrative organization inside the Andean Integration System; and a system of Andean administrative rules and an administrative justice system.

  5. Morphological Diversity of the Colony Produced by Bacteria Proteus mirabilis

    Science.gov (United States)

    Nakahara, Akio; Shimada, Yuji; Wakita, Jun-ichi; Matsushita, Mitsugu; Matsuyama, Tohey

    1996-08-01

    Morphological changes of colonies have been investigatedfor a bacterial strain of Proteus mirabilis, which is a famous speciesfor producing concentric-ring-like colonies. It was found that colony patterns can be classified into three types,i.e., cyclic spreading, diffusion-limited growth (DLA-like)and three-dimensional growth (inside the agar medium) patterns. Cyclic spreading patterns can further be classifiedinto three subgroups, i.e., concentric-ring, homogeneous and spatiotemporal patterns. These subgroups were classified by examining the development of colony structure after colonies spread all over petri-dishes. Comparison of the results with thoseof another bacterial species Bacillus subtilis is also discussed.

  6. Volcanism and associated hazards: the Andean perspective

    Science.gov (United States)

    Tilling, R. I.

    2009-12-01

    Andean volcanism occurs within the Andean Volcanic Arc (AVA), which is the product of subduction of the Nazca Plate and Antarctica Plates beneath the South America Plate. The AVA is Earth's longest but discontinuous continental-margin volcanic arc, which consists of four distinct segments: Northern Volcanic Zone, Central Volcanic Zone, Southern Volcanic Zone, and Austral Volcanic Zone. These segments are separated by volcanically inactive gaps that are inferred to indicate regions where the dips of the subducting plates are too shallow to favor the magma generation needed to sustain volcanism. The Andes host more volcanoes that have been active during the Holocene (past 10 000 years) than any other volcanic region in the world, as well as giant caldera systems that have produced 6 of the 47 largest explosive eruptions (so-called "super eruptions") recognized worldwide that have occurred from the Ordovician to the Pleistocene. The Andean region's most powerful historical explosive eruption occurred in 1600 at Huaynaputina Volcano (Peru). The impacts of this event, whose eruptive volume exceeded 11 km3, were widespread, with distal ashfall reported at distances >1000 km away. Despite the huge size of the Huaynaputina eruption, human fatalities from hazardous processes (pyroclastic flows, ashfalls, volcanogenic earthquakes, and lahars) were comparatively small owing to the low population density at the time. In contrast, lahars generated by a much smaller eruption (Colombia) killed about 25 000 people - the worst volcanic disaster in the Andean region as well as the second worst in the world in the 20th century. The Ruiz tragedy has been attributed largely to ineffective communications of hazards information and indecisiveness by government officials, rather than any major deficiencies in scientific data. Ruiz's disastrous outcome, however, together with responses to subsequent hazardous eruptions in Chile, Colombia, Ecuador, and Peru has spurred significant

  7. Pharmacological and biological activities of Mirabilis jalapa L.

    Directory of Open Access Journals (Sweden)

    Rozina Rozina

    2016-05-01

    Full Text Available AbstractPlants have been used for health care and medical purposes for several thousands of years. The number of higher plant species on earth is about 250000. It is estimated that 35000 to 70000 species have, at one time or another, been used in some cultures for medicinal purposes. One of these is Mirabilis jalapa L. which is traditionally used as Purgative and emetic, for treatment of many gastrointestinal disorders, including dysentery, diarrhea, muscle pain and abdominal colic. Besides this it also exhibits certain useful activities like Antiviral activity, Antimicrobial activity, Antimalarial activity, Anthelmintic activity, Antioxidant activity, Aytotoxic activity Anti-tubercular drugs induced hepatotoxicity, Antinociceptive activity, Antifungal activity, Anti-corrosion activity, Antispasmodic activity, Anti-inflammatory activity and many others. Pharmacologically active compounds include active alcoholic extract, ether compound-3,3’-Methylenebis (4- hydroxycoumarin N-D-alpha-Phenylyglycinelaminaribiitol-3-(4-(dimethylamino cinnamoyl 4hydroxycoumarin. The purpose of my review is to find out the areas of scope and to give the detail of the work done on Mirabilis Jalapa L. for future research work.

  8. Proteus mirabilis viability after lithotripsy of struvite calculi

    Science.gov (United States)

    Prabakharan, Sabitha; Teichman, Joel M. H.; Spore, Scott S.; Sabanegh, Edmund; Glickman, Randolph D.; McLean, Robert J. C.

    2000-05-01

    Urinary calculi composed of struvite harbor urease-producing bacteria within the stone. The photothermal mechanism of holmium:YAG lithotripsy is uniquely different than other lithotripsy devices. We postulated that bacterial viability of struvite calculi would be less for calculi fragmented with holmium:YAG irradiation compared to other lithotripsy devices. Human calculi of known struvite composition (greater than 90% magnesium ammonium phosphate hexahydrate) were incubated with Proteus mirabilis. Calculi were fragmented with no lithotripsy (controls), or shock wave, intracorporeal ultrasonic, electrohydraulic, pneumatic, holmium:YAG or pulsed dye laser lithotripsy. After lithotripsy, stone fragments were sonicated and specimens were serially plated for 48 hours at 38 C. Bacterial counts and the rate of bacterial sterilization were compared. Median bacterial counts (colony forming units per ml) were 8 X 106 in controls and 3 X 106 in shock wave, 3 X 107 in ultrasonic, 4 X 105 in electrohydraulic, 8 X 106 in pneumatic, 5 X 104 in holmium:YAG and 1 X 106 in pulsed dye laser lithotripsy, p less than 0.001. The rate of bacterial sterilization was 50% for holmium:YAG lithotripsy treated stones versus 0% for each of the other cohorts, p less than 0.01. P. mirabilis viability is less after holmium:YAG irradiation compared to other lithotripsy devices.

  9. Loss of FliL alters Proteus mirabilis surface sensing and temperature-dependent swarming.

    Science.gov (United States)

    Lee, Yi-Ying; Belas, Robert

    2015-01-01

    Proteus mirabilis is a dimorphic motile bacterium well known for its flagellum-dependent swarming motility over surfaces. In liquid, P. mirabilis cells are 1.5- to 2.0-μm swimmer cells with 4 to 6 flagella. When P. mirabilis encounters a solid surface, where flagellar rotation is limited, swimmer cells differentiate into elongated (10- to 80-μm), highly flagellated swarmer cells. In order for P. mirabilis to swarm, it first needs to detect a surface. The ubiquitous but functionally enigmatic flagellar basal body protein FliL is involved in P. mirabilis surface sensing. Previous studies have suggested that FliL is essential for swarming through its involvement in viscosity-dependent monitoring of flagellar rotation. In this study, we constructed and characterized ΔfliL mutants of P. mirabilis and Escherichia coli. Unexpectedly and unlike other fliL mutants, both P. mirabilis and E. coli ΔfliL cells swarm (Swr(+)). Further analysis revealed that P. mirabilis ΔfliL cells also exhibit an alteration in their ability to sense a surface: e.g., ΔfliL P. mirabilis cells swarm precociously over surfaces with low viscosity that normally impede wild-type swarming. Precocious swarming is due to an increase in the number of elongated swarmer cells in the population. Loss of fliL also results in an inhibition of swarming at <30°C. E. coli ΔfliL cells also exhibit temperature-sensitive swarming. These results suggest an involvement of FliL in the energetics and function of the flagellar motor. Copyright © 2015, American Society for Microbiology. All Rights Reserved.

  10. The fabrication of andean particularism

    Directory of Open Access Journals (Sweden)

    1989-01-01

    original culture through resistance, it focuses on the fabrication of tradition within the disciplinary strategies of the colonial order and on the local re-employment of those productions in political mobilizations. The discussion of this major issue within the field of Andean history is undertaken against the horizon of a case of resistance focusing on the Inca from the Audiencia of Quito in the seventeenth century. Such an incident affords a privileged field for the investigation of the connections between colonial authority and origins, since it stages the recall, simulation and reinstatement of the past.

  11. The Andean Swallow (Orochelidon andecola) in Argentina

    OpenAIRE

    Mazar Barnett, Juan; Pugnali, Germán D.; Pearman Morrison, Mark; Bodrati, Alejandro; Moschione, Flavio; Clark, Ricardo; Roesler, Carlos Ignacio; Monteleone, Diego; Casañas, Hernán; Burgos Gallardo, Freddy; Segovia, José; Pagano, Luis; Povedano, Hernán; Areta, Juan Ignacio

    2016-01-01

    During ornithological studies in the provinces of Jujuy, Salta, and San Juan, we recorded the Andean Swallow Orochelidon andecola at 40 localities. These are the first records in Argentina, and also represent the southernmost for the species. Some of these localities are up to 1500 m lower than the previously known elevational limit (now 800 masl), and up to 1100 km southwards. This is a relatively poorly known swallow, and we present novel natural history data. We found evidence of breeding ...

  12. Subcutaneous infection by Ochroconis mirabilis in an immunocompetent patient

    Directory of Open Access Journals (Sweden)

    Dongmei Shi

    2016-03-01

    Full Text Available Recently, the taxonomy of Ochroconis (Ascomycota, Pezizomycotina, Venturiales, Sympoventuriaceae has been revised with the recognition of an additional genus, Verruconis. Ochroconis comprises mesophilic saprobes that occasionally infect vertebrates which mostly are cold-blooded, while Verruconis contains thermophilic species which is a neurotrope in humans and birds. On the basis of molecular data it is noted that only a single Ochroconis species regularly infects immunocompetent human hosts. Here we report a subcutaneous infection due to Ochroconis mirabilis in a 50-year-old immunocompetent female patient. In vitro antifungal susceptibility tests revealed that terbinafine was the most effective drug. The patient was successfully cured with oral administration of terbinafine 250 mg daily in combination with 3 times of topical ALA-photodynamic therapy for 9 months.

  13. Pathological and therapeutic significance of cellular invasion by Proteus mirabilis in an enterocystoplasty infection stone model

    NARCIS (Netherlands)

    R.B. Mathoera (Rejiv); D.J. Kok (Dirk); C.M. Verduin (Cees); R.J.M. Nijman (Rien)

    2002-01-01

    textabstractProteus mirabilis infection often leads to stone formation. We evaluated how bacterium-mucin adhesion, invasion, and intracellular crystal formation are related to antibiotic sensitivity and may cause frequent stone formation in enterocystoplasties. Five intestinal

  14. Title: High-level cefotaxime-resistant Proteus mirabilis strain isolated ...

    African Journals Online (AJOL)

    oaca

    High-level cefotaxime-resistant Proteus mirabilis strain isolated from a Tunisian .... UV- visible) at 37°C. Specific activity is calculated on depending of. Ross and ..... Performance standards for antimicrobial susceptibility testing;. Seventeenth ...

  15. Molecular Analysis, Biochemical Characterization, Antimicrobial Activity, and Immunological Analysis of Proteus mirabilis Isolated from Broilers.

    Science.gov (United States)

    Yeh, Hung-Yueh; Line, John E; Hinton, Arthur

    2018-03-01

    Proteus mirabilis, a Gram-negative bacterium, is ubiquitous in the environment and is considered as the normal microflora in the human gastrointestinal tract. However, this bacterium is an opportunistic pathogen in humans, often causing urinary tract infections. Moreover, Proteus has been frequently isolated from food animals, including poultry. Whether this bacterium contributes to the foodborne illness in humans is unclear. In this report, P. mirabilis isolates recovered from broilers during housing in the units were characterized, their antimicrobial activity was assayed, and broiler immune response to the soluble proteins was determined. Cecal contents and fecal droppings were treated according to the standard protocol for isolation. Speciation based on biochemical reactions and the antimicrobial activity of the isolates were carried out using commercial kits. Immunoblot was assayed to determine immune status of broilers against P. mirabilis. A total of 10 isolates of P. mirabilis were selected for further characterization. These isolates could grow in pH 6.0 and 1% NaCl conditions. They were resistant to sodium lactate, troleandomycin, rifamycin SV, vancomycin, but sensitive to nalidixic acid, cefotaxime and novobiocin. Moreover, the CTX, ACC, CMY-1, BIC, NDM, VEB, qnrB and qnrD genes were detected by PCR amplification in all isolates. Sera from broilers harboring this bacterium reacted to the P. mirabilis soluble proteins, but not from litter- and age-matched P. mirabilis negative and SPF chickens, indicating that this bacterium infected chickens that could have humoral immune response against P. mirabilis. This study provides a rationale for further monitoring P. mirabilis during poultry production to determine whether this bacterium poses potential threats to public health. Published 2018. This article is a U.S. Government work and is in the public domain in the USA.

  16. Analysis of pathogenic factors of Proteus mirabilis isolated from urinary tract infection

    OpenAIRE

    室谷,勝久

    1991-01-01

    Proteus mirabilis has several pathogenic factors such as adherent ability to urinary tract epitherial cells, urease, motility and resistance to urine. The pathogenic activities of clinically isolated P. mirabilis were analyzed. Higher pathogenic strains (No. 25 and No. 30) which had morphologically different pili but had a higher density of pili showed strong adherent activity to bladder epithelial cells of mouse and rat. These strains also showed a clear chemotaxis to urinary tract tissue ex...

  17. Involvement of polyphosphate kinase in virulence and stress tolerance of uropathogenic Proteus mirabilis.

    Science.gov (United States)

    Peng, Liang; Jiang, Qiao; Pan, Jia-Yun; Deng, Cong; Yu, Jing-Yi; Wu, Xiao-Man; Huang, Sheng-He; Deng, Xiao-Yan

    2016-04-01

    Proteus mirabilis (P. mirabilis), a gram-negative enteric bacterium, frequently causes urinary tract infections. Many virulence factors of uropathogenic P. mirabilis have been identified, including urease, flagella, hemolysin and fimbriae. However, the functions of polyphosphate kinase (PPK), which are related to the pathogenicity of many bacteria, remain entirely unknown in P. mirabilis. In this study, a ppk gene encoding the PPK insertional mutant in P. mirabilis strain HI4320 was constructed, and its biological functions were examined. The results of survival studies demonstrated that the ppk mutant was deficient in resistance to oxidative, hyperosmotic and heat stress. The swarming and biofilm formation abilities of P. mirabilis were also attenuated after the ppk interruption. In vitro and in vivo experiments suggested that ppk was required for P. mirabilis to invade the bladder. The negative phenotypes of the ppk mutant could be restored by ppk gene complementation. Furthermore, two-dimensional gel electrophoresis and liquid chromatography-mass spectrometry were used to analyze the proteomes of the wild-type strain and the ppk mutant. Compared with the wild-type strain, seven proteins including TonB-dependent receptor, universal stress protein G, major mannose-resistant/Proteus-like fimbrial protein (MR/P fimbriae), heat shock protein, flagellar capping protein, putative membrane protein and multidrug efflux protein were down-regulated, and four proteins including exported peptidase, repressor protein for FtsI, FKBP-type peptidyl-prolyl cis-trans isomerase and phosphotransferase were up-regulated in the ppk mutant. As a whole, these results indicate that PPK is an important regulator and plays a crucial role in stress tolerance and virulence in uropathogenic P. mirabilis.

  18. Antimicrobial activity Study of triclosan-loaded WBPU on Proteus mirabilis in vitro.

    Science.gov (United States)

    Tian, Ye; Jian, Zhongyu; Wang, Jianzhong; He, Wei; Liu, Qinyu; Wang, Kunjie; Li, Hong; Tan, Hong

    2017-04-01

    To evaluate the antimicrobial activity study of triclosan-loaded waterborne polyurethanes (WBPU) on Proteus mirabilis in vitro. Inhibition zone assays on petri plates with triclosan-loaded WBPU samples were used to test its antimicrobial activity on Proteus mirabilis. Models of the catheterized bladder supplied with artificial urine infected with Proteus mirabilis were employed to confirm the antimicrobial activity of triclosan-loaded WBPU. Bacteria colony counting, pH of the residual urine at each time point and catheter blockage time were recorded. Confocal laser scanning microscopy, scanning electron microscopy and encrustation deposits dry weighing were used for evaluating the biofilm formation. Inhibition zones formed in the triclosan-loaded WBPU groups in a dose-response manner (the radius for samples with 1, 0.1 and 0.01 mg triclosan were 9.93 ± 1.08, 6.07 ± 0.54 and 2.47 ± 0.25 mm, P Proteus mirabilis biofilm formation (33.9 ± 13.9 mg vs. 1.4 ± 1.5 mg, P = 0.016). Triclosan-loaded WBPU significantly inhibited Proteus mirabilis' growth and biofilm formation, indicating the promising antibacterial effects on Proteus mirabilis in vitro. Further efforts are under way that involves coating the material onto the urinary catheters and in vivo studies.

  19. Greek rheumatoid arthritis patients have elevated levels of antibodies against antigens from Proteus mirabilis.

    Science.gov (United States)

    Christopoulos, Georgios; Christopoulou, V; Routsias, J G; Babionitakis, A; Antoniadis, C; Vaiopoulos, G

    2017-03-01

    Patients with rheumatoid arthritis (RA) from different ethnic groups present elevated levels of antibodies against Proteus mirabilis. This finding implicates P. mirabilis in the development of RA. The aim of this study was to investigate the importance of P. mirabilis in the etiopathogenesis of RA in Greek RA patients. In this study, 63 patients with RA and 38 healthy controls were included. Class-specific antibodies IgM, IgG, and IgA against three human cross-reactive and non-cross-reactive synthetic peptides from P. mirabilis-hemolysin (HpmB), urease C (UreC), and urease F (UreF)-were performed in all subjects, using the ELISA method. RA patients had elevated levels of IgM, IgG, and IgA antibodies against HpmB and UreC Proteus peptide which are significantly different compared to healthy controls: p = 0.005, p Proteus peptide-which are non-cross-reactive with human tissue antigens-were observed and their significant difference compared to healthy controls (p = 0.007, p mirabilis antigenic epitopes, such as in North European populations, albeit Greek RA patients presenting the cross-reaction antigen in a low percentage. These results indicate that P. mirabilis through the molecular mimicry mechanism leads to inflammation and damage of the joints in RA.

  20. Marking behavior of Andean bears in an Ecuadorian cloud forest

    NARCIS (Netherlands)

    Filipczyková, Eva; Heitkonig, Ignas; Castellanos, Armando; Hantson, Wouter; Steyaert, Sam M.J.G.

    2017-01-01

    Very little is known about marking behavior of the endangered Andean bear (Tremarctos ornatus). Here, we present a first detailed description of Andean bear marking behavior obtained using camera traps. From November 2012 to April 2013, we inspected 16 bear trails in the Napo province of eastern

  1. Physico-chemical and sensory properties of marmalades made from mixtures of fruits and under-exploited Andean tubers.

    Science.gov (United States)

    Lotufo Haddad, Agustina M; Margalef, María Isabel; Armada, Margarita; Goldner, María Cristina

    2017-09-01

    This work studies the added value to the Andean tuber crops through the production of jams. The objective were: (1) to study the sensory and instrumental characteristics of dietetic marmalades made with fruits and Andean tubers; (2) to research consumer's acceptability and emotional responses; (3) to assess the relationship between sensory and instrumental variables and (4) to determine sensory, instrumental and emotional variables that influence the acceptability. Pearson's correlations showed that spreadability was the variable better predicted by sensory and instrumental ones. The analysis of variance showed that sourness increased with the increase of strawberry and the decrease of apple contents (P analysis of sensory/acceptability/emotion data, 'typical', 'autochthonous', 'urban' and 'present' resulted in positive emotions which favored the consumer liking. Formulating marmalades with mixtures of fruits and Andean tubers will allow giving value-added to these crops. The elaboration of products using innovative raw materials will be an incentive for farmers to cultivate them. © 2017 Society of Chemical Industry. © 2017 Society of Chemical Industry.

  2. Regional outbreak of CTX-M-2 β-lactamase-producing Proteus mirabilis in Japan.

    Science.gov (United States)

    Nakano, Ryuichi; Nakano, Akiyo; Abe, Michiko; Inoue, Matsuhisa; Okamoto, Ryoichi

    2012-12-01

    Proteus mirabilis is a common cause of urinary tract infection. Wild-type P. mirabilis strains are usually susceptible to penicillins and cephalosporins, but occurrences of P. mirabilis producing extended-spectrum β-lactamases (ESBLs) have been recently reported. Here, we surveyed the prevalence of cefotaxime resistance among P. mirabilis strains at seven different hospitals in Kanagawa Prefecture, Japan, and investigated their molecular epidemiology to explain the mechanism of their spread. The prevalence of cefotaxime resistance among P. mirabilis increased annually, from 10.1 % in 1998 to 23.1 % in 2003, and increased drastically in 2004, exceeding 40 %. We collected 105 consecutive and non-duplicate cefotaxime-resistant P. mirabilis isolates (MIC 16 to >256 µg ml(-1)) from these hospitals from June 2004 to May 2005 and characterized their profile. PCR and sequence analysis revealed that all resistant strains produced exclusively CTX-M-2 β-lactamase. PFGE analysis identified 47 banding patterns with 83 % or greater similarity. These results indicated that a regional outbreak of P. mirabilis producing CTX-M-2 β-lactamase has occurred in Japan and suggest that the epidemic spread occurred within and across hospitals and communities by extended clonal strains. Plasmid analysis revealed that 44.8 % of plasmids harboured by bla(CTX-M-2) isolates had common profiles, encoding ISEcp1, IS26 and Int1, and belonged to incompatibility group T. Spread of the resistant isolates in Japan resulted from dissemination of narrow-host-range plasmids of the IncT group encoding bla(CTX-M-2). These findings indicate the rapidly developing problem of treating the species to prevent dissemination of ESBL producers.

  3. A novel functional class 2 integron in clinical Proteus mirabilis isolates.

    Science.gov (United States)

    Wei, Quhao; Hu, Qingfeng; Li, Shanshan; Lu, Huoyang; Chen, Guoqiang; Shen, Beiqiong; Zhang, Ping; Zhou, Yonglie

    2014-04-01

    To describe a novel functional class 2 integron that was found in clinical Proteus mirabilis isolates. Class 1 and 2 integrons were screened by PCR in 153 clinical Proteus isolates. The variable regions of class 1 and 2 integrons were determined by restriction analysis and sequencing. The mutations of internal stop codons in class 2 integrons and their common promoters were also determined by sequencing. Enterobacterial repetitive intergenic consensus (ERIC)-PCR was used to analyse the phylogenetic relations of class 2 integron-positive P. mirabilis isolates. Class 1 integrons were detected in 96 (63%) of 153 Proteus isolates: eight different gene cassette arrays were detected, including dfrA32-ereA1-aadA2, which was detected for the first time in P. mirabilis. Class 2 integrons were detected in 101 (66%) of 153 Proteus isolates: four different gene cassette arrays were detected, including dfrA1-catB2-sat2-aadA1, which was detected for the first time in a class 2 integron. A novel functional class 2 integron was detected in 38 P. mirabilis isolates with a common promoter (-35 TTTAAT|16 bp|-10 TAAAGT). The variable region of this functional class 2 integron contained dfrA14 and three novel open reading frames with unknown functions. Very similar ERIC-PCR fingerprinting patterns were detected in these 38 P. mirabilis isolates and were different from other class 2 integron-positive isolates. A novel functional class 2 integron was found for the first time in P. mirabilis. These functional class 2 integron-harbouring P. mirabilis isolates were likely to be clonally spread in our hospital.

  4. Unity and Difference in Andean Songs

    Directory of Open Access Journals (Sweden)

    Charles Maurice Pigott

    2013-03-01

    Full Text Available This essay explores the concepts of “unity” and “difference” in Andean songs. The verses pertain to the Masha ritual enacted annually in Mangas, central Peru, and combine Quechua (the indigenous language with Spanish. Through detailed exegesis of the texts, this essay argues that, far from being irreconcilable, “unity” and “difference” are best understood as mutually informing since the recognition of difference opens up the parameters of potential exchange. This optic is informed by a worldview that emphasizes “relation” over “entities.”

  5. Detection of KPC-2 in a Clinical Isolate of Proteus mirabilis and First Reported Description of Carbapenemase Resistance Caused by a KPC Beta-Lactamase in P. mirabilis

    Science.gov (United States)

    An isolate of Proteus mirabilis recovered from bacterial cultures was shown to be resistant to imipenem, meropenem, and ertapenem by disk diffusion susceptibility testing. Amplification of whole cell and/or plasmid DNA recovered from the isolate using primers specific for the blaKPC carbapenemase g...

  6. Theory of periodic swarming of bacteria: Application to Proteus mirabilis

    Science.gov (United States)

    Czirók, A.; Matsushita, M.; Vicsek, T.

    2001-03-01

    The periodic swarming of bacteria is one of the simplest examples for pattern formation produced by the self-organized collective behavior of a large number of organisms. In the spectacular colonies of Proteus mirabilis (the most common species exhibiting this type of growth), a series of concentric rings are developed as the bacteria multiply and swarm following a scenario that periodically repeats itself. We have developed a theoretical description for this process in order to obtain a deeper insight into some of the typical processes governing the phenomena in systems of many interacting living units. Our approach is based on simple assumptions directly related to the latest experimental observations on colony formation under various conditions. The corresponding one-dimensional model consists of two coupled differential equations investigated here both by numerical integrations and by analyzing the various expressions obtained from these equations using a few natural assumptions about the parameters of the model. We determine the phase diagram corresponding to systems exhibiting periodic swarming, and discuss in detail how the various stages of the colony development can be interpreted in our framework. We point out that all of our theoretical results are in excellent agreement with the complete set of available observations. Thus the present study represents one of the few examples where self-organized biological pattern formation is understood within a relatively simple theoretical approach, leading to results and predictions fully compatible with experiments.

  7. Keanekaragaman Mirabilis jalapa L. Berdasarkan Pola Pita Isozim Peroksidase

    Directory of Open Access Journals (Sweden)

    AHMAD DWI SETYAWAN

    2007-05-01

    Full Text Available Mirabilis jalapa L. is a gardening plant which growth easily planted because their beautiful color and flower’s ornaments. The aims of the research were to find out the variation of isozyme band of peroxidase (PER and their close relationship of M. jalapa cultivated based on their different flower’s color. The method used in this study was polyacrilamide gel electrophoresis. The collecting data were analyzed using calculation of association coefficient (Gower’s coefficient with cluster analysis used for similarities of their arithmetic. The results showed that in M. jalapa were found 21 variation of isozyme bands of peroxidase (PER from 8 Rf of 0.04; 0.08; 0.20; 0.28; 0.36; 0.48; 0,60; and 0.64. M. jalapa with white and red nodes flower’s (BIN and white and red line flower’s (GAR have the nearest genetic relationship with similarity index of 0.76. M. jalapa with white and red nodes flower’s (BIN and white and red line flower’s (GAR joined with red flower’s (MER on 0.73 of similarity index then joined with white flower’s (PUT on 0.64 of similarity index and yellow flower’s (KUN on0.49 of similarity index. At least, M. jalapa with orange flower’s (ORA joined with M. jalapa cultivars on 0.41 of similarity index.

  8. Environmental scanning electron microscopy analysis of Proteus mirabilis biofilms grown on chitin and stainless steel.

    Science.gov (United States)

    Fernández-Delgado, Milagro; Duque, Zoilabet; Rojas, Héctor; Suárez, Paula; Contreras, Monica; García-Amado, María A; Alciaturi, Carlos

    Proteus mirabilis is a human pathogen able to form biofilms on the surface of urinary catheters. Little is known about P. mirabilis biofilms on natural or industrial surfaces and the potential consequences for these settings. The main aim of this work was to assess and compare the adhesion and biofilm formation of P. mirabilis strains from different origins on chitin and stainless steel surfaces within 4 to 96 h. Using environmental scanning electron microscopy, the biofilms of a clinical strain grown on chitin at 4 h showed greater adhesion, aggregation, thickness, and extracellular matrix production than those grown on stainless steel, whereas biofilms of an environmental strain had less aggregation on both surfaces. Biofilms of both P. mirabilis strains developed different structures on chitin, such as pillars, mushrooms, channels, and crystalline-like precipitates between 24 and 96 h, in contrast with flat-layer biofilms produced on stainless steel. Significant differences ( p  biofilm formation. This represents the first study of P. mirabilis showing adhesion, biofilm formation, and development of different structures on surfaces found outside the human host.

  9. Proteus mirabilis biofilm - qualitative and quantitative colorimetric methods-based evaluation.

    Science.gov (United States)

    Kwiecinska-Piróg, Joanna; Bogiel, Tomasz; Skowron, Krzysztof; Wieckowska, Ewa; Gospodarek, Eugenia

    2014-01-01

    Proteus mirabilis strains ability to form biofilm is a current topic of a number of research worldwide. In this study the biofilm formation of P. mirabilis strains derived from urine of the catheterized and non-catheterized patients has been investigated. A total number of 39 P. mirabilis strains isolated from the urine samples of the patients of dr Antoni Jurasz University Hospital No. 1 in Bydgoszcz clinics between 2011 and 2012 was used. Biofilm formation was evaluated using two independent quantitative and qualitative methods with TTC (2,3,5-triphenyl-tetrazolium chloride) and CV (crystal violet) application. The obtained results confirmed biofilm formation by all the examined strains, except quantitative method with TTC, in which 7.7% of the strains did not have this ability. It was shown that P. mirabilis rods have the ability to form biofilm on the surfaces of both biomaterials applied, polystyrene and polyvinyl chloride (Nelaton catheters). The differences in ability to form biofilm observed between P. mirabilis strains derived from the urine of the catheterized and non-catheterized patients were not statistically significant.

  10. Allicin from garlic inhibits the biofilm formation and urease activity of Proteus mirabilis in vitro.

    Science.gov (United States)

    Ranjbar-Omid, Mahsa; Arzanlou, Mohsen; Amani, Mojtaba; Shokri Al-Hashem, Seyyedeh Khadijeh; Amir Mozafari, Nour; Peeri Doghaheh, Hadi

    2015-05-01

    Several virulence factors contribute to the pathogenesis of Proteus mirabilis. This study determined the inhibitory effects of allicin on urease, hemolysin and biofilm of P. mirabilis ATCC 12453 and its antimicrobial activity against 20 clinical isolates of P. mirabilis. Allicin did not inhibit hemolysin, whereas it did inhibit relative urease activity in both pre-lysed (half-maximum inhibitory concentration, IC50 = 4.15 μg) and intact cells (IC50 = 21 μg) in a concentration-dependent manner. Allicin at sub-minimum inhibitory concentrations (2-32 μg mL(-1)) showed no significant effects on the growth of the bacteria (P > 0.05), but it reduced biofilm development in a concentration-dependent manner (P mirabilis isolates were determined to be 128 and 512 μg mL(-1), respectively. The results suggest that allicin could have clinical applications in controlling P. mirabilis infections. © FEMS 2015. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  11. Proteus mirabilis biofilm - Qualitative and quantitative colorimetric methods-based evaluation

    Directory of Open Access Journals (Sweden)

    Joanna Kwiecinska-Piróg

    2014-12-01

    Full Text Available Proteus mirabilis strains ability to form biofilm is a current topic of a number of research worldwide. In this study the biofilm formation of P. mirabilis strains derived from urine of the catheterized and non-catheterized patients has been investigated. A total number of 39 P. mirabilis strains isolated from the urine samples of the patients of dr Antoni Jurasz University Hospital No. 1 in Bydgoszcz clinics between 2011 and 2012 was used. Biofilm formation was evaluated using two independent quantitative and qualitative methods with TTC (2,3,5-triphenyl-tetrazolium chloride and CV (crystal violet application. The obtained results confirmed biofilm formation by all the examined strains, except quantitative method with TTC, in which 7.7% of the strains did not have this ability. It was shown that P. mirabilis rods have the ability to form biofilm on the surfaces of both biomaterials applied, polystyrene and polyvinyl chloride (Nelaton catheters. The differences in ability to form biofilm observed between P. mirabilis strains derived from the urine of the catheterized and non-catheterized patients were not statistically significant.

  12. Flagellum density regulates Proteus mirabilis swarmer cell motility in viscous environments.

    Science.gov (United States)

    Tuson, Hannah H; Copeland, Matthew F; Carey, Sonia; Sacotte, Ryan; Weibel, Douglas B

    2013-01-01

    Proteus mirabilis is an opportunistic pathogen that is frequently associated with urinary tract infections. In the lab, P. mirabilis cells become long and multinucleate and increase their number of flagella as they colonize agar surfaces during swarming. Swarming has been implicated in pathogenesis; however, it is unclear how energetically costly changes in P. mirabilis cell morphology translate into an advantage for adapting to environmental changes. We investigated two morphological changes that occur during swarming--increases in cell length and flagellum density--and discovered that an increase in the surface density of flagella enabled cells to translate rapidly through fluids of increasing viscosity; in contrast, cell length had a small effect on motility. We found that swarm cells had a surface density of flagella that was ∼5 times larger than that of vegetative cells and were motile in fluids with a viscosity that inhibits vegetative cell motility. To test the relationship between flagellum density and velocity, we overexpressed FlhD(4)C(2), the master regulator of the flagellar operon, in vegetative cells of P. mirabilis and found that increased flagellum density produced an increase in cell velocity. Our results establish a relationship between P. mirabilis flagellum density and cell motility in viscous environments that may be relevant to its adaptation during the infection of mammalian urinary tracts and movement in contact with indwelling catheters.

  13. From Catheter to Kidney Stone: The Uropathogenic Lifestyle of Proteus mirabilis.

    Science.gov (United States)

    Norsworthy, Allison N; Pearson, Melanie M

    2017-04-01

    Proteus mirabilis is a model organism for urease-producing uropathogens. These diverse bacteria cause infection stones in the urinary tract and form crystalline biofilms on indwelling urinary catheters, frequently leading to polymicrobial infection. Recent work has elucidated how P. mirabilis causes all of these disease states. Particularly exciting is the discovery that this bacterium forms large clusters in the bladder lumen that are sites for stone formation. These clusters, and other steps of infection, require two virulence factors in particular: urease and MR/P fimbriae. Highlighting the importance of MR/P fimbriae is the cotranscribed regulator, MrpJ, which globally controls virulence. Overall, P. mirabilis exhibits an extraordinary lifestyle, and further probing will answer exciting basic microbiological and clinically relevant questions. Copyright © 2016 Elsevier Ltd. All rights reserved.

  14. Necrotizing suppurative nephritis in a Japanese black feedlot steer due to Proteus mirabilis infection.

    Science.gov (United States)

    Abe, Tadatsugu; Iizuka, Ayako; Kojima, Hirokazu; Kimura, Kumiko; Shibahara, Tomoyuki; Haritani, Makoto

    2017-04-05

    A Japanese black feedlot steer suddenly died after exhibiting astasia and cramping of the extremities. Necropsy of the animal revealed that the right kidney was enlarged and pale with severe nephrolithiasis. The urinary bladder displayed mucosal hemorrhage. Upon bacteriological investigation, Proteus mirabilis was isolated from the liver, spleen, right kidney, lungs and urine. Histopathological examination revealed necrotizing suppurative nephritis with the presence of numerous gram-negative bacilli and fibrinous suppurative cystitis with no bacilli. Immunohistochemical analysis revealed that the bacteria and cytoplasm of the macrophages stained positively with P. mirabilis antiserum. Electron microscopy revealed the presence of numerous bacteria in the renal tubules. To our knowledge, this is the first report describing the histopathological aspects of nephritis caused by P. mirabilis in cattle.

  15. Effects of ceftazidime and ciprofloxacin on biofilm formation in Proteus mirabilis rods.

    Science.gov (United States)

    Kwiecińska-Piróg, Joanna; Bogiel, Tomasz; Gospodarek, Eugenia

    2013-10-01

    Proteus mirabilis rods are one of the most commonly isolated species of the Proteus genus from human infections, mainly those from the urinary tract and wounds. They are often related to biofilm structure formation. The bacterial cells of the biofilm are less susceptible to routinely used antimicrobials, making the treatment more difficult. The aim of this study was to evaluate quantitatively the influence of ceftazidime and ciprofloxacin on biofilm formation on the polyvinyl chloride surface by 42 P. mirabilis strains isolated from urine, purulence, wound swab and bedsore samples. It has been shown that ceftazidime and ciprofloxacin at concentrations equal to 1/4, 1/2 and 1 times their MIC values for particular Proteus spp. strains decrease their ability to form biofilms. Moreover, ciprofloxacin at concentrations equal to 1/4, 1/2 and 1 times their MIC values for particular P. mirabilis strains reduces biofilm formation more efficiently than ceftazidime at the corresponding concentration values.

  16. Molecular detection of HpmA and HlyA hemolysin of uropathogenic Proteus mirabilis.

    Science.gov (United States)

    Cestari, Silvia Emanoele; Ludovico, Marilucia Santos; Martins, Fernando Henrique; da Rocha, Sérgio Paulo Dejato; Elias, Waldir Pereira; Pelayo, Jacinta Sanchez

    2013-12-01

    Urinary tract infection (UTI) is one of the bacterial infections frequently documented in humans. Proteus mirabilis is associated with UTI mainly in individuals with urinary tract abnormality or related with vesicular catheterism and it can be difficult to treat because of the formation of stones in the bladder and kidneys. These stones are formed due to the presence of urease synthesized by the bacteria. Another important factor is that P. mirabilis produces hemolysin HpmA, used by the bacteria to damage the kidney tissues. Proteus spp. samples can also express HlyA hemolysin, similar to that found in Escherichia coli. A total of 211 uropathogenic P. mirabilis isolates were analyzed to detect the presence of the hpmA and hpmB genes by the techniques of polymerase chain reaction (PCR) and dot blot and hlyA by PCR. The hpmA and hpmB genes were expressed by the RT-PCR technique and two P. mirabilis isolates were sequenced for the hpmA and hpmB genes. The presence of the hpmA and hpmB genes was confirmed by PCR in 205 (97.15 %) of the 211 isolates. The dot blot confirmed the presence of the hpmA and hpmB genes in the isolates that did not amplify in the PCR. None of the isolates studied presented the hlyA gene. The hpmA and hpmB genes that were sequenced presented 98 % identity with the same genes of the HI4320 P. mirabilis sample. This study showed that the PCR technique has good sensitivity for detecting the hpmA and hpmB genes of P. mirabilis.

  17. Characterization of 17 chaperone-usher fimbriae encoded by Proteus mirabilis reveals strong conservation

    Science.gov (United States)

    Kuan, Lisa; Schaffer, Jessica N.; Zouzias, Christos D.

    2014-01-01

    Proteus mirabilis is a Gram-negative enteric bacterium that causes complicated urinary tract infections, particularly in patients with indwelling catheters. Sequencing of clinical isolate P. mirabilis HI4320 revealed the presence of 17 predicted chaperone-usher fimbrial operons. We classified these fimbriae into three groups by their genetic relationship to other chaperone-usher fimbriae. Sixteen of these fimbriae are encoded by all seven currently sequenced P. mirabilis genomes. The predicted protein sequence of the major structural subunit for 14 of these fimbriae was highly conserved (≥95 % identity), whereas three other structural subunits (Fim3A, UcaA and Fim6A) were variable. Further examination of 58 clinical isolates showed that 14 of the 17 predicted major structural subunit genes of the fimbriae were present in most strains (>85 %). Transcription of the predicted major structural subunit genes for all 17 fimbriae was measured under different culture conditions designed to mimic conditions in the urinary tract. The majority of the fimbrial genes were induced during stationary phase, static culture or colony growth when compared to exponential-phase aerated culture. Major structural subunit proteins for six of these fimbriae were detected using MS of proteins sheared from the surface of broth-cultured P. mirabilis, demonstrating that this organism may produce multiple fimbriae within a single culture. The high degree of conservation of P. mirabilis fimbriae stands in contrast to uropathogenic Escherichia coli and Salmonella enterica, which exhibit greater variability in their fimbrial repertoires. These findings suggest there may be evolutionary pressure for P. mirabilis to maintain a large fimbrial arsenal. PMID:24809384

  18. Role of DAF-21protein in Caenorhabditis elegans immunity against Proteus mirabilis infection.

    Science.gov (United States)

    JebaMercy, Gnanasekaran; Durai, Sellegounder; Prithika, Udayakumar; Marudhupandiyan, Shanmugam; Dasauni, Pushpanjali; Kundu, Suman; Balamurugan, Krishnaswamy

    2016-08-11

    Caenorhabditis elegans is emerging as one of the handy model for proteome related studies due to its simplest system biology. The present study, deals with changes in protein expression in C. elegans infected with Proteus mirabilis. Proteins were separated using two-dimensional differential gel electrophoresis (2D-DIGE) and identified using MALDI-TOF. Twelve distinctly regulated proteins identified in the infected worms, included heat shock proteins involved stress pathway (HSP-1 and HSP-6), proteins involved in immune response pathway (DAF-21), enzymes involved in normal cellular process (Eukaryotic translation Elongation Factor, actin family member, S-adenosyl homocysteine hydrolase ortholog, glutamate dehydrogenase and Vacuolar H ATPase family member) and few least characterized proteins (H28O16.1 and H08J11.2). The regulation of selected players at the transcriptional level during Proteus mirabilis infection was analyzed using qPCR. Physiological experiments revealed the ability of P. mirabilis to kill daf-21 mutant C. elegans significantly compared with the wild type. This is the first report studying proteome changes in C. elegans and exploring the involvement of MAP Kinase pathway during P. mirabilis infection. This is the first report studying proteome changes in C. elegans during P. mirabilis infection. The present study explores the role and contribution of MAP Kinase pathway and its regulator protein DAF-21 involvement in the immunity against opportunistic pathogen P. mirabilis infection. Manipulation of this DAF-21 protein in host, may pave the way for new drug development or disease control strategy during opportunistic pathogen infections. Copyright © 2016 Elsevier B.V. All rights reserved.

  19. Comparison of phenotypic and virulence genes characteristics in human and chicken isolates of Proteus mirabilis.

    Science.gov (United States)

    Barbour, Elie K; Hajj, Zahi G; Hamadeh, Shadi; Shaib, Houssam A; Farran, Mohamad T; Araj, George; Faroon, Obaid; Barbour, Kamil E; Jirjis, Faris; Azhar, Esam; Kumosani, Taha; Harakeh, Steve

    2012-10-01

    The objective of this work is to compare the phenotypic and virulence genes characteristics in human and chicken isolates of Proteus mirabilis. The bacterial examination of 50 livers of individual broilers, marketed by four major outlets, revealed a high recovery of P. mirabilis (66%), and a low recovery frequency of Salmonella spp. (4%), Serratia odorifera (2%), Citrobacter brakii (2%), and Providencia stuartii (2%). The phenotypic biochemical characterization of the recovered 33 chicken isolates of P. mirabilis were compared to 30 human isolates (23 urinary and six respiratory isolates). The comparison revealed significant differences in the presence of gelatinase enzyme (100% presence in chicken isolates versus 91.3 and 83.3% presence in human urinary and respiratory isolates, respectively, P,0.05). The H(2)S production occurred in 100% of chicken isolates versus 95.6 and 66.7% presence in human urinary and respiratory isolates, respectively, P,0.05). The other 17 biochemical characteristics did not differ significantly among the three groups of isolates (P.0.05). Two virulence genes, the mrpA and FliL, were having a typical 100% presence in randomly selected isolates of P. mirabilis recovered from chicken livers (N510) versus isolates recovered from urinary (N55) and respiratory specimens of humans (N55) (P.0.05). The average percentage similarity of mrpA gene nucleotide sequence of poultry isolates to human urinary and respiratory isolates was 93.2 and 97.5-%, respectively. The high similarity in phenotypic characteristics, associated with typical frequency of presence of two virulence genes, and high similarity in sequences of mrpA gene among poultry versus human P. mirabilis isolates justifies future investigations targeting the evaluation of adaptable pathogenicity of avian Proteus mirabilis isolates to mammalian hosts.

  20. The new Andean Regional Office of Astronomy for Development (ROAD)

    Science.gov (United States)

    Char, Farid; Forero-Romero, Jaime

    2015-08-01

    The Andean Regional Office of Astronomy for Development (ROAD) is a new effort in South America to serve several goals in astronomical development. Six countries (Bolivia, Colombia, Chile, Ecuador, Perú and Venezuela) will work together, representing a common language block in the Andean region and focusing on develop strategies to strengthen the professional research, education and popularization of astronomy. Our current Working Structure comprises a ROAD Coordinator and Coordinators per Task Force, as well as Organizing Committees, Collaborators and Volunteers.The participating institutions of this new ROAD have been involved in many projects involving each of the current OAD’s Task Forces: research, schools and children and public, exploring educational activities/material to be shared among the Andean countries, standardizing the knowledge and creating inspirational experiences. We expect to generate many efforts in order to bring a more homogeneous activity in each Andean country, taking into account the special role of Chile in global astronomy, due to its great conditions for astronomy and the involvement of many professional observatories, universities and astronomy institutions.Our current (and upcoming) most relevant activities includes: Andean Schools on Astronomy, Andean Graduate Program and Massive Open Online Courses (TF1); Virtual Training Sessions and Teaching material for the visually impaired students; Annual TF2 meeting to gather all the collaborators (TF2); Development for planetariums and Communicating Astronomy with the Public (TF3). The Andean region, in the other hand, will also be involved in at least two important events: the CAP Meeting in May 2016 and the XV LARIM in October 2016 (both in Colombia); and Chile will bid to host the XXXI IAU GA in 2021, with the aim of show the great advances in astronomical development from the Andean region and South America.

  1. Recognizing rural territorial heritage: characterization of Andean tuber production systems in Boyacá

    Directory of Open Access Journals (Sweden)

    Clavijo Ponce Neidy Lorena

    2011-08-01

    Full Text Available

    In the municipalities Ventaquemada and Turmequé (Boyacá- Colombia, we identified 20 small agricultures by their production systems including the following Andean tubers: Ullucus tuberosus Caldas (ulluco, Oxalis tuberosa Molina (oca, and Tropaeolum tuberosum R. & P. (Mashua, which were important in their family meals and culture, however, their use has declined and the area has not seen research and development processes that provide alternatives for handling, conservation, use and marketing, and now are at the risk of disappearing. This research conducted participatory assessment processes for the characterization of production systems and initiated reassessment processes of territorial heritage, identifying common sub-farm agrobiodiversity and projects for these traditional Andean tuber crops in order to enhance the special and knowhow knowledge surrounding the production.

  2. The assessment of Proteus mirabilis susceptibility to ceftazidime and ciprofloxacin and the impact of these antibiotics at subinhibitory concentrations on Proteus mirabilis biofilms.

    Science.gov (United States)

    Kwiecińska-Piróg, Joanna; Skowron, Krzysztof; Zniszczol, Katarzyna; Gospodarek, Eugenia

    2013-01-01

    Rods of the Proteus genus are commonly isolated from patients, especially from the urinary tracts of the catheterised patients. The infections associated with biomaterials are crucial therapeutic obstacles, due to the bactericidal resistance of the biofilm. The aim of this study was to assess the susceptibility of P. mirabilis planktonic forms to ciprofloxacin and ceftazidime, the ability to form biofilm, and the impact of chosen sub-MIC concentrations of these antibiotics on biofilm at different stages of its formation. The research included 50 P. mirabilis strains isolated from wounds and the urinary tracts from patients of the University Hospital No. 1 in Bydgoszcz. The assessment of susceptibility to ciprofloxacin and ceftazidime was conducted using micromethods. The impact of sub-MIC concentrations of the chosen antibiotics on the biofilm was measured using the TTC method. The resistance to ciprofloxacin was confirmed for 20 strains (40.0%) while to ceftazidime for 32 (64.0%) of the tested P. mirabilis strains. All of the tested strains formed biofilm: 24.0% weakly, 26.0% moderately, and 50.0% strongly. It was determined that ciprofloxacin and ceftazidime caused eradication of the biofilm. Moreover, the connection between origin of the strains, biofilm maturity level, and resistance to antibiotics was proved.

  3. The Assessment of Proteus mirabilis Susceptibility to Ceftazidime and Ciprofloxacin and the Impact of These Antibiotics at Subinhibitory Concentrations on Proteus mirabilis Biofilms

    Science.gov (United States)

    Kwiecińska-Piróg, Joanna; Zniszczol, Katarzyna; Gospodarek, Eugenia

    2013-01-01

    Rods of the Proteus genus are commonly isolated from patients, especially from the urinary tracts of the catheterised patients. The infections associated with biomaterials are crucial therapeutic obstacles, due to the bactericidal resistance of the biofilm. The aim of this study was to assess the susceptibility of P. mirabilis planktonic forms to ciprofloxacin and ceftazidime, the ability to form biofilm, and the impact of chosen sub-MIC concentrations of these antibiotics on biofilm at different stages of its formation. The research included 50 P. mirabilis strains isolated from wounds and the urinary tracts from patients of the University Hospital No. 1 in Bydgoszcz. The assessment of susceptibility to ciprofloxacin and ceftazidime was conducted using micromethods. The impact of sub-MIC concentrations of the chosen antibiotics on the biofilm was measured using the TTC method. The resistance to ciprofloxacin was confirmed for 20 strains (40.0%) while to ceftazidime for 32 (64.0%) of the tested P. mirabilis strains. All of the tested strains formed biofilm: 24.0% weakly, 26.0% moderately, and 50.0% strongly. It was determined that ciprofloxacin and ceftazidime caused eradication of the biofilm. Moreover, the connection between origin of the strains, biofilm maturity level, and resistance to antibiotics was proved. PMID:24151628

  4. Protective immunity induced by the vaccination of recombinant Proteus mirabilis OmpA expressed in Pichia pastoris.

    Science.gov (United States)

    Zhang, Yongbing; Yang, Shifa; Dai, Xiumei; Liu, Liping; Jiang, Xiaodong; Shao, Mingxu; Chi, Shanshan; Wang, Chuanwen; Yu, Cuilian; Wei, Kai; Zhu, Ruiliang

    2015-01-01

    Proteus mirabilis (P. mirabilis) is a zoonotic pathogen that has recently presented a rising infection rate in the poultry industry. To develop an effective vaccine to protect chickens against P. mirabilis infection, OmpA, one of the major outer membrane proteins of P. mirabilis, was expressed in Pichia pastoris. The concentration of the expressed recombinant OmpA protein reached 8.0μg/mL after induction for 96h with 1.0% methanol in the culture. In addition, OmpA protein was confirmed by SDS-PAGE and Western blot analysis using the antibody against Escherichia coli-expressed OmpA protein. Taishan Pinus massoniana pollen polysaccharide, a known plant-derived adjuvant, was mixed into the recombinant OmpA protein to prepare the OmpA subunit vaccine. We then subcutaneously inoculated this vaccine into chickens to examine the immunoprotective effects. ELISA analysis indicated that an excellent antibody response against OmpA was elicited in the vaccinated chickens. Moreover, a high protection rate of 80.0% was observed in the vaccinated group, which was subsequently challenged with P. mirabilis. The results suggest that the eukaryotic P. mirabilis OmpA was an ideal candidate protein for developing an effective subunit vaccine against P. mirabilis infection. Copyright © 2014 Elsevier Inc. All rights reserved.

  5. Biofilm Formation and Immunomodulatory Activity of Proteus mirabilis Clinically Isolated Strains

    Directory of Open Access Journals (Sweden)

    Alessandra Fusco

    2017-02-01

    Full Text Available Urinary tract infections (UTIs and catheter-associated UTIs (CAUTIs are the principal hospital-acquired infections. Proteus mirabilis is characterized by several virulence factors able to promote adhesion and biofilm formation and ameliorate the colonization of urinary tract and the formation of crystalline biofilms on the abiotic surface of the urinary catheters. Since, to date, the role of P. mirabilis in the etiopathogenesis of different types of urinary tract infections is not well established, in this study we sought to characterize two different clinically isolated strains of P. mirabilis (PM1 and PM2 with distinctive phenotypes and analyzed various virulence factors possibly implicated in the ability to induce UTIs and CAUTIs. In particular, we analyzed motility, biofilm formation both on abiotic and biotic surfaces of PM1 and PM2 and paralleled these parameters with the ability to induce an inflammatory response in an epithelial cell model. Results showed that PM1 displayed major motility and a capacity to form biofilm and was associated with an anti-inflammatory response of host cells. Conversely, PM2 exhibited lack motility and a had slower organization in biofilm but promoted an increase of proinflammatory cytokine expression in infected epithelial cells. Our study provides data useful to start uncovering the pathologic basis of P. mirabilis-associated urinary infections. The evidence of different virulence factors expressed by PM1 and PM2 highlights the possibility to use precise and personalized therapies targeting specific virulence pathways.

  6. The functional localization of cytochromes b in the respiratory chain of anaerobically grown Proteus mirabilis

    NARCIS (Netherlands)

    Van Wielink, J E; Reijnders, W N; Van Spanning, R J; Oltmann, L F; Stouthamer, A.H.

    1986-01-01

    The functional localization of the cytochromes b found in anaerobically grown Proteus mirabilis was investigated. From light absorption spectra, scanned during uninhibited and HQNO-inhibited electron transport to various electron acceptors, it was concluded that all cytochromes b function between

  7. Anaerobic choline metabolism in microcompartments promotes growth and swarming of Proteus mirabilis.

    Science.gov (United States)

    Jameson, Eleanor; Fu, Tiantian; Brown, Ian R; Paszkiewicz, Konrad; Purdy, Kevin J; Frank, Stefanie; Chen, Yin

    2016-09-01

    Gammaproteobacteria are important gut microbes but only persist at low levels in the healthy gut. The ecology of Gammaproteobacteria in the gut environment is poorly understood. Here, we demonstrate that choline is an important growth substrate for representatives of Gammaproteobacteria. Using Proteus mirabilis as a model, we investigate the role of choline metabolism and demonstrate that the cutC gene, encoding a choline-trimethylamine lyase, is essential for choline degradation to trimethylamine by targeted mutagenesis of cutC and subsequent complementation experiments. Proteus mirabilis can rapidly utilize choline to enhance growth rate and cell yield in broth culture. Importantly, choline also enhances swarming-associated colony expansion of P. mirabilis under anaerobic conditions on a solid surface. Comparative transcriptomics demonstrated that choline not only induces choline-trimethylamine lyase but also genes encoding shell proteins for the formation of bacterial microcompartments. Subsequent analyses by transmission electron microscopy confirmed the presence of such novel microcompartments in cells cultivated in liquid broth and hyper-flagellated swarmer cells from solid medium. Together, our study reveals choline metabolism as an adaptation strategy for P. mirabilis and contributes to better understand the ecology of this bacterium in health and disease. © 2015 The Authors. Environmental Microbiology published by Society for Applied Microbiology and John Wiley & Sons Ltd.

  8. Genome sequence of a Proteus mirabilis strain isolated from the salivary glands of larval Lucilia sericata

    Science.gov (United States)

    We announced a draft genome sequence of a Proteus mirabilis strain derived from Lucilia sericata salivary glands. This strain is demonstrated to attract and induce oviposition by L. sericata, a common blow fly important to medicine, agriculture, and forensics. The genome will help to dissect inter...

  9. Biofilm Formation and Immunomodulatory Activity of Proteus mirabilis Clinically Isolated Strains.

    Science.gov (United States)

    Fusco, Alessandra; Coretti, Lorena; Savio, Vittoria; Buommino, Elisabetta; Lembo, Francesca; Donnarumma, Giovanna

    2017-02-15

    Urinary tract infections (UTIs) and catheter-associated UTIs (CAUTIs) are the principal hospital-acquired infections. Proteus mirabilis is characterized by several virulence factors able to promote adhesion and biofilm formation and ameliorate the colonization of urinary tract and the formation of crystalline biofilms on the abiotic surface of the urinary catheters. Since, to date, the role of P. mirabilis in the etiopathogenesis of different types of urinary tract infections is not well established, in this study we sought to characterize two different clinically isolated strains of P. mirabilis (PM1 and PM2) with distinctive phenotypes and analyzed various virulence factors possibly implicated in the ability to induce UTIs and CAUTIs. In particular, we analyzed motility, biofilm formation both on abiotic and biotic surfaces of PM1 and PM2 and paralleled these parameters with the ability to induce an inflammatory response in an epithelial cell model. Results showed that PM1 displayed major motility and a capacity to form biofilm and was associated with an anti-inflammatory response of host cells. Conversely, PM2 exhibited lack motility and a had slower organization in biofilm but promoted an increase of proinflammatory cytokine expression in infected epithelial cells. Our study provides data useful to start uncovering the pathologic basis of P. mirabilis -associated urinary infections. The evidence of different virulence factors expressed by PM1 and PM2 highlights the possibility to use precise and personalized therapies targeting specific virulence pathways.

  10. Thanatosis as an adaptive male mating strategy in the nuptial gift-giving spider Pisaura mirabilis

    DEFF Research Database (Denmark)

    Hansen, Line S.; Gonzalez, Sofía F.; Toft, Søren

    2008-01-01

    Males and females often experience different optima in mating rate, which may cause evolution of female resistance to matings and male counter adaptations to increase mating rate. Males of the spider Pisaura mirabilis display a spectacular mating behavior involving a nuptial gift and thanatosis...

  11. Tracking spatial variation in river load from Andean highlands to inter-Andean valleys

    Science.gov (United States)

    Tenorio, Gustavo E.; Vanacker, Veerle; Campforts, Benjamin; Álvarez, Lenín; Zhiminaicela, Santiago; Vercruysse, Kim; Molina, Armando; Govers, Gerard

    2018-05-01

    Mountains play an important role in the denudation of continents and transfer erosion and weathering products to lowlands and oceans. The rates at which erosion and weathering processes take place in mountain regions have a substantial impact on the morphology and biogeochemistry of downstream reaches and lowlands. The controlling factors of physical erosion and chemical weathering and the coupling between the two processes are not yet fully understood. In this study, we report physical erosion and chemical weathering rates for five Andean catchments located in the southern Ecuadorian Andes and investigate their mutual interaction. During a 4-year monitoring period, we sampled river water at biweekly intervals, and we analyzed water samples for major ions and suspended solids. We derived the total annual dissolved, suspended sediment, and ionic loads from the flow frequency curves and adjusted rating curves and used the dissolved and suspended sediment yields as proxies for chemical weathering and erosion rates. In the 4-year period of monitoring, chemical weathering exceeds physical erosion in the high Andean catchments. Whereas physical erosion rates do not exceed 30 t km-2 y-1 in the relict glaciated morphology, chemical weathering rates range between 22 and 59 t km-2 y-1. The variation in chemical weathering is primarily controlled by intrinsic differences in bedrock lithology. Land use has no discernible impact on the weathering rate but leads to a small increase in base cation concentrations because of fertilizer leaching in surface water. When extending our analysis with published data on dissolved and suspended sediment yields from the northern and central Andes, we observe that the river load composition strongly changes in the downstream direction, indicating large heterogeneity of weathering processes and rates within large Andean basins.

  12. Optimization of a pulsed-field gel electrophoresis for molecular typing of Proteus mirabilis

    Directory of Open Access Journals (Sweden)

    Alper Karagöz

    2013-09-01

    Full Text Available Objective: For the detection of outbreaks caused byProteus mirabilis, strains clonal relations are determinedmethods as “pulsed-field gel electrophoresis (PFGE”.The aim of this study was optimization of a pulsed-fieldgel electrophoresis for molecular typing of P. mirabilis.Methods: In this study, PFGE’ protocol is optimized foruse in molecular typing of P. mirabilis. Phylogenetic analyzesof strains were evaluated with Bionumerics softwaresystem (version 6.01; Applied Maths, Sint-Martens-Latem, Belgium.Results: This protocol compared with Gram-negativebacteria PFGE protocols, NotI enzyme is suitable for thisbacterium. Electrophoresis conditions should be revealedas; - block 1: initial pulse duration 1 sec, ending pulseduration 30 sec, striking angle 120°, the current 6 V/cm2,temperature 14°C, time 8 hours; - block 2: initial pulseduration 30 sec, ending pulse duration 70 sec, strikingangle 120°, the current 6 V/cm2, temperature 14°C, time16 hours; - TBE, pH=8.4.Conclusion: P. mirabilis strains were typed by PFGE andBionumerics analysis program were determined clonal relationships.The procedure was simple, reproducible andsuitable for these bacteria. Also it was evaluated, becauseof reducing time, the solution volumes and enzymes canbe economically. Outbreaks of nosocomial infections dueto bacteria studied assessment and the potential to provideuseful information about the degree of prevalence.This optimized protocol is allowed different centers’ PFGEresults to compare with other laboratories results. J ClinExp Invest 2013; 4 (3: 306-312Key words: Proteus mirabilis, molecular typing, pulsedfieldgel electrophoresis.

  13. Emergence of Salmonella genomic island 1 (SGI1) among Proteus mirabilis clinical isolates in Dijon, France.

    Science.gov (United States)

    Siebor, Eliane; Neuwirth, Catherine

    2013-08-01

    Salmonella genomic island 1 (SGI1) is often encountered in antibiotic-resistant Salmonella enterica and exceptionally in Proteus mirabilis. We investigated the prevalence of SGI1-producing clinical isolates of P. mirabilis in our hospital (Dijon, France). A total of 57 strains of P. mirabilis resistant to amoxicillin and/or gentamicin and/or trimethoprim/sulfamethoxazole isolated from August 2011 to February 2012 as well as 9 extended-spectrum β-lactamase (ESBL)-producing P. mirabilis from our collection were tested for the presence of SGI1 by PCR. The complete SGI1 structure from positive isolates [backbone and multidrug resistance (MDR) region] was sequenced. SGI1 was detected in 7 isolates; 5 out of the 57 isolates collected during the study period (9%) and 2 out of the 9 ESBL-producing strains of our collection. The structures of the seven SGI1s were distinct. Three different backbones were identified: one identical to the SGI1 backbone from the epidemic Salmonella Typhimurium DT104, one with variations already described in SGI1-K from Salmonella Kentucky (deletion and insertion of IS1359 in the region spanning from S005 to S009) and one with a variation never detected before (deletion from S005 to S009). Six different MDR regions were identified: four simple variants containing resistance genes already described and two variants harbouring a very complex structure including regions derived from several transposons and IS26 elements with aphA1a never reported to date in SGI1. SGI1 variants are widely distributed among P. mirabilis clinical strains and might spread to other commensal Enterobacteriaceae. This would become a serious public health problem.

  14. Dissemination of blaOXA-58 in Proteus mirabilis isolates from Germany.

    Science.gov (United States)

    Lange, Felix; Pfennigwerth, Niels; Gerigk, Sonja; Gohlke, Frank; Oberdorfer, Klaus; Purr, Ingvill; Wohanka, Nikolaus; Roggenkamp, Andreas; Gatermann, Sören G; Kaase, Martin

    2017-05-01

    Characterization of Proteus mirabilis isolates harbouring bla OXA-58 with emphasis on the genetic environment of this resistance determinant. Strains of P. mirabilis ( n  =   37) isolated from different patients were tested for the presence of bla OXA-58 . The genetic context of bla OXA-58 was determined by WGS of two strains and Sanger sequencing. Clonality of the strains was assessed by PFGE. Susceptibility testing was performed by microdilution according to EUCAST. Four strains isolated in different geographical regions of Germany were positive for bla OXA-58 , and WGS showed that this resistance gene was harboured on a plasmid. Sanger sequencing confirmed the presence of two nearly identical plasmids, 6219 and 6208 bp in size, in all four strains. Upstream of bla OXA-58 an IS Aba 3-like transposase gene was located. The P. mirabilis strains were not clonally related according to PFGE. MICs of meropenem for three of the strains were only just above the EUCAST breakpoint and the Carba NP test was positive for only two of the strains. To our knowledge, this is the first description of bla OXA-58 in the species P. mirabilis . The resistance gene is harboured by almost identical plasmids in strains not clonally related and from different geographical regions. Apart from an IS Aba 3-like transposase gene upstream of bla OXA-58 the genetic context is different from bla OXA-58 harboured on plasmids in the genus Acinetobacter . With MICs of meropenem well below the EUCAST breakpoint or only just above it and equivocal or false negative results from the Carba NP test, bla OXA-58 can be easily overlooked in P. mirabilis . © The Author 2017. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  15. The Pathogenic Potential of Proteus mirabilis Is Enhanced by Other Uropathogens during Polymicrobial Urinary Tract Infection.

    Science.gov (United States)

    Armbruster, Chelsie E; Smith, Sara N; Johnson, Alexandra O; DeOrnellas, Valerie; Eaton, Kathryn A; Yep, Alejandra; Mody, Lona; Wu, Weisheng; Mobley, Harry L T

    2017-02-01

    Urinary catheter use is prevalent in health care settings, and polymicrobial colonization by urease-positive organisms, such as Proteus mirabilis and Providencia stuartii, commonly occurs with long-term catheterization. We previously demonstrated that coinfection with P. mirabilis and P. stuartii increased overall urease activity in vitro and disease severity in a model of urinary tract infection (UTI). In this study, we expanded these findings to a murine model of catheter-associated UTI (CAUTI), delineated the contribution of enhanced urease activity to coinfection pathogenesis, and screened for enhanced urease activity with other common CAUTI pathogens. In the UTI model, mice coinfected with the two species exhibited higher urine pH values, urolithiasis, bacteremia, and more pronounced tissue damage and inflammation compared to the findings for mice infected with a single species, despite having a similar bacterial burden within the urinary tract. The presence of P. stuartii, regardless of urease production by this organism, was sufficient to enhance P. mirabilis urease activity and increase disease severity, and enhanced urease activity was the predominant factor driving tissue damage and the dissemination of both organisms to the bloodstream during coinfection. These findings were largely recapitulated in the CAUTI model. Other uropathogens also enhanced P. mirabilis urease activity in vitro, including recent clinical isolates of Escherichia coli, Enterococcus faecalis, Klebsiella pneumoniae, and Pseudomonas aeruginosa We therefore conclude that the underlying mechanism of enhanced urease activity may represent a widespread target for limiting the detrimental consequences of polymicrobial catheter colonization, particularly by P. mirabilis and other urease-positive bacteria. Copyright © 2017 American Society for Microbiology.

  16. The Andean Geotrail (1): A scientific adventure

    Science.gov (United States)

    Sassier, C.; Galland, O.; Raufaste, C.; Mair, K.

    2009-12-01

    The role of Geosciences in our society is of primary importance. Its implications for humanity relate to major challenges such as climate change, managing energy resources, natural hazard mitigation, and water scarcity. Despite these issues being familiar to specialists, this is in general not the case for the public. In a world, where the impact of human activity is beginning to be seen on the environment, knowledge of the Earth and its history is paramount to make informed decisions that will influence our future. The necessity to educate the global population and raise awareness of Geosciences has led UNESCO to designate 2009 the International Year of the Planet Earth. In this context and with the label of the UNESCO, we organized and performed a popular science adventure that was followed in real time by both school children and many adults around the world. The Andean Geotrail consisted of a cycling expedition through a spectacular geological environment, the Andean Cordillera. During the nine month expedition, we cycled 8000 km and walked 400 km from Ushuaia in the Southern tip of Argentina to Nazca in Peru to encounter a rich variety of geological environments: active volcanoes, earthquakes, mineral and hydrocarbon deposits, and fantastic geological scenery. All this makes the Andes a great pedagogical natural laboratory. During the expedition, we visited spectacular geological localities that illustrate key Earth Science phenomena (such as mines and hydrocarbon deposits, erupting volcanoes and seismogenically active areas, and national parks) and discovered their implications for the local people. Along the way, we interviewed local geologists and scientists who helped us understand the geology of their areas. We gathered our own observations with those of the local specialists and published essays, articles and photographs on our website and blog (www.georouteandine.fr/English, http://georouteandine.blogspot.com). Seventeen schools in France and Norway

  17. Andean tectonics: Implications for Satellite Geodesy

    Science.gov (United States)

    Allenby, R. J.

    1984-01-01

    Current knowledge and theories of large scale Andean tectonics as they relate to site planning for the NASA Crustal Dynamics Program's proposed high precision geodetic measurements of relative motions between the Nazca and South American plates are summarized. The Nazca Plate and its eastern margin, the Peru-Chile Trench, is considered a prototype plate marked by rapid motion, strong seismicity and well defined boundaries. Tectonic activity across the Andes results from the Nazca Plate subducting under the South American plate in a series of discrete platelets with different widths and dip angles. This in turn, is reflected in the tectonic complexity of the Andes which are a multitutde of orogenic belts superimposed on each other since the Precambrian. Sites for Crustal Dynamics Program measurements are being located to investigate both interplate and extraplate motions. Observing operations have already been initiated at Arequipa, Peru and Easter Island, Santiago and Cerro Tololo, Chile. Sites under consideration include Iquique, Chile; Oruro and Santa Cruz, Bolivia; Cuzco, Lima, Huancayo and Bayovar, Peru; and Quito and the Galapagos Islands, Ecuador. Based on scientific considerations, Santa Cruz, Huancayo (or Lima), Quito and the Galapagos Islands should be replaced by Isla San Felix, Chile; Brazilia or Petrolina, Brazil; and Guayaquil, Ecuador. If resources permit, additional important sites would be Buenaventura and Villavicencio or Puerto La Concordia, Colombia; and Mendoza and Cordoba, Argentina.

  18. Environment, vulnerability, and gender in Andean ethnomedicine.

    Science.gov (United States)

    Larme, A C

    1998-10-01

    In Cuyo Cuyo, in the southern Peruvian highlands, ethnomedicine is rife with images of human vulnerability to a hostile and unpredictable environment. This is represented in the ethnomedical system by a focus on wayras, air- or wind-borne illnesses that enter through vulnerable body openings such as the head, orifices, lower back, and feet. Women are viewed to be more vulnerable, or débil, than men to illness because they have an extra orifice, the vagina, they lose copious amounts of blood, which is thought to be irreplaceable, during childbirth. and because they suffer more negative emotions, which are thought to attract wayras and other illnesses to the body. The relationship of ethnomedical beliefs to the Andean physical and political economic environment is explored within the context of social and economic change. Negative beliefs about women's bodies have negative effects on women's roles and position vis-à-vis men in present day Cuyo Cuyo. Ethnomedical beliefs reflect and reinforce gender inequalities in present day Peru and are part of a cultural ideology that in general devalues women. This case study demonstrates that power is a key dimension in the cultural construction of medical knowledge. whether in non-Western or Western societies.

  19. Alternative crops

    International Nuclear Information System (INIS)

    Andreasen, L.M.; Boon, A.D.

    1992-01-01

    Surplus cereal production in the EEC and decreasing product prices, mainly for cereals, has prompted considerable interest for new earnings in arable farming. The objective was to examine whether suggested new crops (fibre, oil, medicinal and alternative grains crops) could be considered as real alternatives. Whether a specific crop can compete economically with cereals and whether there is a market demand for the crop is analyzed. The described possibilities will result in ca. 50,000 hectares of new crops. It is expected that they would not immediately provide increased earnings, but in the long run expected price developments are more positive than for cereals. The area for new crops will not solve the current surplus cereal problem as the area used for new crops is only 3% of that used for cereals. Preconditions for many new crops is further research activities and development work as well as the establishment of processing units and organizational initiatives. Presumably, it is stated, there will then be a basis for a profitable production of new crops for some farmers. (AB) (47 refs.)

  20. On the Nature of Cross-Linguistic Transfer: A Case Study of Andean Spanish

    Science.gov (United States)

    Muntendam, Antje G.

    2013-01-01

    This paper presents the results of a study on cross-linguistic transfer in Andean Spanish word order. In Andean Spanish the object appears in preverbal position more frequently than in non-Andean Spanish, which has been attributed to an influence from Quechua (a Subject-Object-Verb language). The high frequency of preverbal objects could be…

  1. Novel Insights into the Proteus mirabilis Crystalline Biofilm Using Real-Time Imaging.

    Directory of Open Access Journals (Sweden)

    Sandra A Wilks

    Full Text Available The long-term use of indwelling catheters results in a high risk from urinary tract infections (UTI and blockage. Blockages often occur from crystalline deposits, formed as the pH rises due to the action of urease-producing bacteria; the most commonly found species being Proteus mirabilis. These crystalline biofilms have been found to develop on all catheter materials with P. mirabilis attaching to all surfaces and forming encrustations. Previous studies have mainly relied on electron microscopy to describe this process but there remains a lack of understanding into the stages of biofilm formation. Using an advanced light microscopy technique, episcopic differential interference contrast (EDIC microscopy combined with epifluorescence (EF, we describe a non-destructive, non-contact, real-time imaging method used to track all stages of biofilm development from initial single cell attachment to complex crystalline biofilm formation. Using a simple six-well plate system, attachment of P. mirabilis (in artificial urine to sections of silicone and hydrogel latex catheters was tracked over time (up to 24 days. Using EDIC and EF we show how initial attachment occurred in less than 1 h following exposure to P. mirabilis. This was rapidly followed by an accumulation of an additional material (indicated to be carbohydrate based using lectin staining and the presence of highly elongated, motile cells. After 24 h exposure, a layer developed above this conditioning film and within 4 days the entire surface (of both catheter materials was covered with diffuse crystalline deposits with defined crystals embedded. Using three-dimensional image reconstruction software, cells of P. mirabilis were seen covering the crystal surfaces. EDIC microscopy could resolve these four components of the complex crystalline biofilm and the close relationship between P. mirabilis and the crystals. This real-time imaging technique permits study of this complex biofilm development

  2. Novel Insights into the Proteus mirabilis Crystalline Biofilm Using Real-Time Imaging.

    Science.gov (United States)

    Wilks, Sandra A; Fader, Mandy J; Keevil, C William

    2015-01-01

    The long-term use of indwelling catheters results in a high risk from urinary tract infections (UTI) and blockage. Blockages often occur from crystalline deposits, formed as the pH rises due to the action of urease-producing bacteria; the most commonly found species being Proteus mirabilis. These crystalline biofilms have been found to develop on all catheter materials with P. mirabilis attaching to all surfaces and forming encrustations. Previous studies have mainly relied on electron microscopy to describe this process but there remains a lack of understanding into the stages of biofilm formation. Using an advanced light microscopy technique, episcopic differential interference contrast (EDIC) microscopy combined with epifluorescence (EF), we describe a non-destructive, non-contact, real-time imaging method used to track all stages of biofilm development from initial single cell attachment to complex crystalline biofilm formation. Using a simple six-well plate system, attachment of P. mirabilis (in artificial urine) to sections of silicone and hydrogel latex catheters was tracked over time (up to 24 days). Using EDIC and EF we show how initial attachment occurred in less than 1 h following exposure to P. mirabilis. This was rapidly followed by an accumulation of an additional material (indicated to be carbohydrate based using lectin staining) and the presence of highly elongated, motile cells. After 24 h exposure, a layer developed above this conditioning film and within 4 days the entire surface (of both catheter materials) was covered with diffuse crystalline deposits with defined crystals embedded. Using three-dimensional image reconstruction software, cells of P. mirabilis were seen covering the crystal surfaces. EDIC microscopy could resolve these four components of the complex crystalline biofilm and the close relationship between P. mirabilis and the crystals. This real-time imaging technique permits study of this complex biofilm development with no risk

  3. qnrA6 genetic environment and quinolone resistance conferred on Proteus mirabilis.

    Science.gov (United States)

    Jayol, Aurélie; Janvier, Frédéric; Guillard, Thomas; Chau, Françoise; Mérens, Audrey; Robert, Jérôme; Fantin, Bruno; Berçot, Béatrice; Cambau, Emmanuelle

    2016-04-01

    To determine the genetic location and environment of the qnrA6 gene in Proteus mirabilis PS16 where it was first described and to characterize the quinolone resistance qnrA6 confers. Transformation experiments and Southern blotting were performed for plasmid and genomic DNA of P. mirabilis PS16 to determine the qnrA6 location. Combinatorial PCRs with primers in qnrA6 and genes usually surrounding qnrA genes were used to determine the genetic environment. The qnrA6 coding region, including or not the promoter region, was cloned into vectors pTOPO and pBR322 and the MICs of six quinolones were measured for transformants of Escherichia coli TOP10 and P. mirabilis ATCC 29906 Rif(R). qnrA6 was shown to be chromosomally encoded in P. mirabilis PS16 and its genetic environment was 81%-87% similar to that of qnrA2 in the Shewanella algae chromosome. The 5138 bp region up- and downstream of qnrA6 contained an IS10 sequence surrounded by two ISCR1. This resulted in qnrA6 being displaced 1.9 kb from its native promoter but supplied a promoter present in ISCR1. qnrA6 cloned into pTOPO and pBR322 conferred a 4-32-fold increase in fluoroquinolone MICs when expressed in E. coli but only 2-3-fold in P. mirabilis. When including the promoter region, a further increase in resistance was observed in both species, reaching MIC values above clinical breakpoints for only P. mirabilis. qnrA6 is the first chromosomally located qnrA gene described in Enterobacteriaceae. The quinolone resistance conferred by qnrA6 depends on the proximity of an efficient promoter and the host strain where it is expressed. © The Author 2016. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

  4. Cloning of a recA-like gene of Proteus mirabilis

    International Nuclear Information System (INIS)

    Eitner, G.; Solonin, A.S.; Tanyashin, V.I.

    1981-01-01

    A gene of Proteus mirabilis that can substitute for functions of the recA gene of Escherichia coli has been cloned into the plasmid pBR322, using shotgun experiments. The recA-like gene (recAsub(P.m.)) has been localized by restriction mapping within a 1.5-Md PstI fragment that is a part of two cloned Hind III fragments of the chromosome of P. mirabilis. The restriction map of the recAsub(P.m.) gene differs from that of the recA gene of E. coli. Funtionally, the recombinant plasmids containing the recAsub(P.m.) gene restore a nearly wild-type level of UV-resistance to several point and deletion mutants in the recA gene of E. coli. (Auth.)

  5. Proteus mirabilis RMS 203 as a new representative of the O13 Proteus serogroup.

    Science.gov (United States)

    Palusiak, Agata; Siwińska, Małgorzata; Zabłotni, Agnieszka

    2015-01-01

    The unique feature of some Proteus O-polysaccharides is occurrence of an amide of galacturonic acid with N(ε)-[(S/R)-1-Carboxyethyl]-L-lysine, GalA6(2S,8S/R-AlaLys). The results of the serological studies presented here, with reference to known O-antigens structures suggest that GalA6(2S,8S/R-AlaLys) or 2S,8R-AlaLys contribute to cross-reactions of O13 Proteus antisera, and Proteeae LPSs. It was also revealed that the Proteus mirabilis RMS 203 strain can be classified into the O13 serogroup, represented so far by two strains: Proteus mirabilis 26/57 and Proteus vulgaris 8344. The O13 LPS is a serologically important antigen with a fragment common to LPSs of different species in the Proteeae tribe.

  6. Mimicking a New 2-Phenylethanol Production Pathway from Proteus mirabilis JN458 in Escherichia coli.

    Science.gov (United States)

    Liu, Jinbin; Jiang, Jing; Bai, Yajun; Fan, Tai-Ping; Zhao, Ye; Zheng, Xiaohui; Cai, Yujie

    2018-04-04

    Bacteria rarely produce natural 2-phenylethanol. We verified a new pathway from Proteus mirabilis JN458 to produce 2-phenylethanol using Escherichia coli to coexpress l-amino acid deaminase, α-keto acid decarboxylase, and alcohol dehydrogenase from P. mirabilis. Based on this pathway, a glucose dehydrogenase coenzyme regeneration system was constructed. The optimal conditions of biotransformation by the recombinant strain E-pAEAKaG were at 40 °C and pH 7.0. Finally, the recombinant strain E-pAEAKaG produced 3.21 ± 0.10 g/L 2-phenylethanol in M9 medium containing 10 g/L l-phenylalanine after a 16 h transformation. Furthermore, when the concentration of l-phenylalanine was 4 g/L (24 mM), the production of 2-phenylethanol reached 2.88 ± 0.18 g/L and displayed a higher conversion rate of 97.38 mol %.

  7. Fourier Transform Infrared Spectroscopy as a Tool in Analysis of Proteus mirabilis Endotoxins.

    Science.gov (United States)

    Żarnowiec, Paulina; Czerwonka, Grzegorz; Kaca, Wiesław

    2017-01-01

    Fourier transform infrared spectroscopy (FT-IR) was used to scan whole bacterial cells as well as lipopolysaccharides (LPSs, endotoxins) isolated from them. Proteus mirabilis cells, with chemically defined LPSs, served as a model for the ATR FT-IR method. The paper focuses on three steps of infrared spectroscopy: (1) sample preparation, (2) IR scanning, and (3) multivariate analysis of IR data (principal component analysis, PCA).

  8. An Unusual Cause of Infective Endocarditis: Proteus mirabilis Bacteremia from an Infected Pressure Ulcer

    Directory of Open Access Journals (Sweden)

    Chun-Hao Liu

    2015-12-01

    Full Text Available Proteus species is a common cause of urinary tract and wound infections in humans. We herein present the case of a 71-year-old male who had fever, a new-onset heart murmur, bacteremia, and a vegetation over his native aortic valve in echocardiography. This rare case demonstrated that infective endocarditis could be caused by Proteus mirabilis from an infected pressure ulcer.

  9. The RNA Chaperone Hfq Is Involved in Stress Tolerance and Virulence in Uropathogenic Proteus mirabilis

    Science.gov (United States)

    Wang, Min-Cheng; Liaw, Shwu-Jen

    2014-01-01

    Hfq is a bacterial RNA chaperone involved in the riboregulation of diverse genes via small noncoding RNAs. Here, we show that Hfq is critical for the uropathogenic Proteus mirabilis to effectively colonize the bladder and kidneys in a murine urinary tract infection (UTI) model and to establish burned wound infection of the rats. In this regard, we found the hfq mutant induced higher IL-8 and MIF levels of uroepithelial cells and displayed reduced intra-macrophage survival. The loss of hfq affected bacterial abilities to handle H2O2 and osmotic pressures and to grow at 50°C. Relative to wild-type, the hfq mutant had reduced motility, fewer flagella and less hemolysin expression and was less prone to form biofilm and to adhere to and invade uroepithelial cells. The MR/P fimbrial operon was almost switched to the off phase in the hfq mutant. In addition, we found the hfq mutant exhibited an altered outer membrane profile and had higher RpoE expression, which indicates the hfq mutant may encounter increased envelope stress. With the notion of envelope disturbance in the hfq mutant, we found increased membrane permeability and antibiotic susceptibilities in the hfq mutant. Finally, we showed that Hfq positively regulated the RpoS level and tolerance to H2O2 in the stationary phase seemed largely mediated through the Hfq-dependent RpoS expression. Together, our data indicate that Hfq plays a critical role in P. mirabilis to establish UTIs by modulating stress responses, surface structures and virulence factors. This study suggests Hfq may serve as a scaffold molecule for development of novel anti-P. mirabilis drugs and P. mirabilis hfq mutant is a vaccine candidate for preventing UTIs. PMID:24454905

  10. Proteus mirabilis inhibits cancer growth and pulmonary metastasis in a mouse breast cancer model.

    Science.gov (United States)

    Zhang, Hong; Diao, Hongxiu; Jia, Lixin; Yuan, Yujing; Thamm, Douglas H; Wang, Huanan; Jin, Yipeng; Pei, Shimin; Zhou, Bin; Yu, Fang; Zhao, Linna; Cheng, Nan; Du, Hongchao; Huang, Ying; Zhang, Di; Lin, Degui

    2017-01-01

    A variety of bacteria have been used as agents and vectors for antineoplastic therapy. A series of mechanisms, including native bacterial toxicity, sensitization of the immune system and competition for nutrients, may contribute to antitumor effects. However, the antitumor effects of Proteus species have been minimally studied, and it is not clear if bacteria can alter tumor hypoxia as a component of their antineoplastic effect. In the present study, Proteus mirabilis bacteria were evaluated for the ability to proliferate and accumulate in murine tumors after intravenous injection. To further investigate the efficacy and safety of bacterial injection, mice bearing 4T1 tumors were treated with an intravenous dose of 5×107 CFU Proteus mirabilis bacteria via the tail vein weekly for three treatments. Histopathology, immunohistochemistry (IHC) and western analysis were then performed on excised tumors. The results suggested Proteus mirabilis localized preferentially to tumor tissues and remarkably suppressed the growth of primary breast cancer and pulmonary metastasis in murine 4T1 models. Results showed that the expression of NKp46 and CD11c was significantly increased after bacteria treatment. Furthermore, tumor expression of carbonic anhydrase IX (CA IX) and hypoxia inducible factor-1a (HIF-1a), surrogates for hypoxia, was significantly lower in the treated group than the control group mice as assessed by IHC and western analysis. These findings demonstrated that Proteus mirabilis may a promising bacterial strain for used against primary tumor growth and pulmonary metastasis, and the immune system and reduction of tumor hypoxia may contribute to the antineoplastic and antimetastatic effects observed.

  11. Case report 496: Intraosseous gas in Proteus mirabilis osteomyelitis complicating bone infarcts in sickle cell disease

    International Nuclear Information System (INIS)

    Marx, A.C.; Hartshorne, M.F.; Stull, M.A.; Truwit, C.L.

    1988-01-01

    Plain film radiographs showed dramatic changes of this rare complication of sickle cell disease in an adult patient, eventually resulting in bilateral amputations of the lower extremities. Cross table radiographs of the knees showed the air-fluid levels without soft tissue swelling or periosteal reaction. The balance of the studies performed confirmed and refined the initial impression that infarction and infection had occurred within the medullary spaces of four long bones. The offending organism was Proteus mirabilis. (orig.)

  12. IMP-27, a Unique Metallo-β-Lactamase Identified in Geographically Distinct Isolates of Proteus mirabilis.

    Science.gov (United States)

    Dixon, Nyssa; Fowler, Randal C; Yoshizumi, A; Horiyama, Tsukasa; Ishii, Y; Harrison, Lucas; Geyer, Chelsie N; Moland, Ellen Smith; Thomson, Kenneth; Hanson, Nancy D

    2016-10-01

    A novel metallo-β-lactamase gene, blaIMP-27, was identified in unrelated Proteus mirabilis isolates from two geographically distinct locations in the United States. Both isolates harbor blaIMP-27 as part of the first gene cassette in a class 2 integron. Antimicrobial susceptibility testing indicated susceptibility to aztreonam, piperacillin-tazobactam, and ceftazidime but resistance to ertapenem. However, hydrolysis assays indicated that ceftazidime was a substrate for IMP-27. Copyright © 2016 Dixon et al.

  13. Case report 496: Intraosseous gas in Proteus mirabilis osteomyelitis complicating bone infarcts in sickle cell disease

    Energy Technology Data Exchange (ETDEWEB)

    Marx, A.C.; Hartshorne, M.F.; Stull, M.A.; Truwit, C.L.

    1988-10-01

    Plain film radiographs showed dramatic changes of this rare complication of sickle cell disease in an adult patient, eventually resulting in bilateral amputations of the lower extremities. Cross table radiographs of the knees showed the air-fluid levels without soft tissue swelling or periosteal reaction. The balance of the studies performed confirmed and refined the initial impression that infarction and infection had occurred within the medullary spaces of four long bones. The offending organism was Proteus mirabilis.

  14. Chromosomal Amplification of the blaOXA-58 Carbapenemase Gene in a Proteus mirabilis Clinical Isolate.

    Science.gov (United States)

    Girlich, Delphine; Bonnin, Rémy A; Bogaerts, Pierre; De Laveleye, Morgane; Huang, Daniel T; Dortet, Laurent; Glaser, Philippe; Glupczynski, Youri; Naas, Thierry

    2017-02-01

    Horizontal gene transfer may occur between distantly related bacteria, thus leading to genetic plasticity and in some cases to acquisition of novel resistance traits. Proteus mirabilis is an enterobacterial species responsible for human infections that may express various acquired β-lactam resistance genes, including different classes of carbapenemase genes. Here we report a Proteus mirabilis clinical isolate (strain 1091) displaying resistance to penicillin, including temocillin, together with reduced susceptibility to carbapenems and susceptibility to expanded-spectrum cephalosporins. Using biochemical tests, significant carbapenem hydrolysis was detected in P. mirabilis 1091. Since PCR failed to detect acquired carbapenemase genes commonly found in Enterobacteriaceae, we used a whole-genome sequencing approach that revealed the presence of bla OXA-58 class D carbapenemase gene, so far identified only in Acinetobacter species. This gene was located on a 3.1-kb element coharboring a bla AmpC -like gene. Remarkably, these two genes were bracketed by putative XerC-XerD binding sites and inserted at a XerC-XerD site located between the terminase-like small- and large-subunit genes of a bacteriophage. Increased expression of the two bla genes resulted from a 6-time tandem amplification of the element as revealed by Southern blotting. This is the first isolation of a clinical P. mirabilis strain producing OXA-58, a class D carbapenemase, and the first description of a XerC-XerD-dependent insertion of antibiotic resistance genes within a bacteriophage. This study revealed a new role for the XerC-XerD recombinase in bacteriophage biology. Copyright © 2017 American Society for Microbiology.

  15. Proteus mirabilis inhibits cancer growth and pulmonary metastasis in a mouse breast cancer model.

    Directory of Open Access Journals (Sweden)

    Hong Zhang

    Full Text Available A variety of bacteria have been used as agents and vectors for antineoplastic therapy. A series of mechanisms, including native bacterial toxicity, sensitization of the immune system and competition for nutrients, may contribute to antitumor effects. However, the antitumor effects of Proteus species have been minimally studied, and it is not clear if bacteria can alter tumor hypoxia as a component of their antineoplastic effect. In the present study, Proteus mirabilis bacteria were evaluated for the ability to proliferate and accumulate in murine tumors after intravenous injection. To further investigate the efficacy and safety of bacterial injection, mice bearing 4T1 tumors were treated with an intravenous dose of 5×107 CFU Proteus mirabilis bacteria via the tail vein weekly for three treatments. Histopathology, immunohistochemistry (IHC and western analysis were then performed on excised tumors. The results suggested Proteus mirabilis localized preferentially to tumor tissues and remarkably suppressed the growth of primary breast cancer and pulmonary metastasis in murine 4T1 models. Results showed that the expression of NKp46 and CD11c was significantly increased after bacteria treatment. Furthermore, tumor expression of carbonic anhydrase IX (CA IX and hypoxia inducible factor-1a (HIF-1a, surrogates for hypoxia, was significantly lower in the treated group than the control group mice as assessed by IHC and western analysis. These findings demonstrated that Proteus mirabilis may a promising bacterial strain for used against primary tumor growth and pulmonary metastasis, and the immune system and reduction of tumor hypoxia may contribute to the antineoplastic and antimetastatic effects observed.

  16. Smooth and rough Proteus mirabilis lipopolysaccharides studied by total internal reflection ellipsometry

    International Nuclear Information System (INIS)

    Gleńska-Olender, J.; Dworecki, K.; Sęk, S.; Kwinkowski, M.; Kaca, W.

    2013-01-01

    Total internal reflection ellipsometry (TIRE), a label-free optical detection technique for studying interactions between biomolecules, was used to examine the adsorption of various forms of lipopolysaccharides (LPSs) isolated from Proteus mirabilis S1959, R110, and R45 strains on a gold surface. The thickness of the adsorbed layers was determined by TIRE, with the average values for S1959, R110, and R45 LPS layers being 78 ± 5, 39 ± 3, and 12 ± 2 nm, respectively. The thickness of LPS layers corresponds to the presence and length of O-specific parts in P. mirabilis LPS molecules. Atomic force microscopy was used as a complementary technique for visualizing lipopolysaccharides on the surface. Force measurements seem to confirm the data obtained from TIRE experiments. - Highlights: • Proteus mirabilis lipopolysaccharides were adsorbed on the gold surface. • Thickness of adsorbed layers was determined by total internal reflection ellipsometry. • Atomic force microscopy was used to visualize lipopolysaccharide build-up on gold surface. • Time is important in the evolution of biomolecular film thickness created on gold surface

  17. Smooth and rough Proteus mirabilis lipopolysaccharides studied by total internal reflection ellipsometry

    Energy Technology Data Exchange (ETDEWEB)

    Gleńska-Olender, J., E-mail: joannaglenska@wp.pl [Institute of Biology, Jan Kochanowski University, 25-406 Kielce (Poland); Świętokrzyski Biobank, Regional Science and Technology Center, 26-060 Chęciny (Poland); Dworecki, K. [Institute of Physics, Jan Kochanowski University, 25-406 Kielce (Poland); Sęk, S. [Department of Chemistry, University of Warsaw, 02-093 Warsaw (Poland); Kwinkowski, M.; Kaca, W. [Institute of Biology, Jan Kochanowski University, 25-406 Kielce (Poland)

    2013-12-02

    Total internal reflection ellipsometry (TIRE), a label-free optical detection technique for studying interactions between biomolecules, was used to examine the adsorption of various forms of lipopolysaccharides (LPSs) isolated from Proteus mirabilis S1959, R110, and R45 strains on a gold surface. The thickness of the adsorbed layers was determined by TIRE, with the average values for S1959, R110, and R45 LPS layers being 78 ± 5, 39 ± 3, and 12 ± 2 nm, respectively. The thickness of LPS layers corresponds to the presence and length of O-specific parts in P. mirabilis LPS molecules. Atomic force microscopy was used as a complementary technique for visualizing lipopolysaccharides on the surface. Force measurements seem to confirm the data obtained from TIRE experiments. - Highlights: • Proteus mirabilis lipopolysaccharides were adsorbed on the gold surface. • Thickness of adsorbed layers was determined by total internal reflection ellipsometry. • Atomic force microscopy was used to visualize lipopolysaccharide build-up on gold surface. • Time is important in the evolution of biomolecular film thickness created on gold surface.

  18. Putrescine importer PlaP contributes to swarming motility and urothelial cell invasion in Proteus mirabilis.

    Science.gov (United States)

    Kurihara, Shin; Sakai, Yumi; Suzuki, Hideyuki; Muth, Aaron; Phanstiel, Otto; Rather, Philip N

    2013-05-31

    Previously, we reported that the speA gene, encoding arginine decarboxylase, is required for swarming in the urinary tract pathogen Proteus mirabilis. In addition, this previous study suggested that putrescine may act as a cell-to-cell signaling molecule (Sturgill, G., and Rather, P. N. (2004) Mol. Microbiol. 51, 437-446). In this new study, PlaP, a putative putrescine importer, was characterized in P. mirabilis. In a wild-type background, a plaP null mutation resulted in a modest swarming defect and slightly decreased levels of intracellular putrescine. In a P. mirabilis speA mutant with greatly reduced levels of intracellular putrescine, plaP was required for the putrescine-dependent rescue of swarming motility. When a speA/plaP double mutant was grown in the presence of extracellular putrescine, the intracellular levels of putrescine were greatly reduced compared with the speA mutant alone, indicating that PlaP functioned as the primary putrescine importer. In urothelial cell invasion assays, a speA mutant exhibited a 50% reduction in invasion when compared with wild type, and this defect could be restored by putrescine in a PlaP-dependent manner. The putrescine analog Triamide-44 partially inhibited the uptake of putrescine by PlaP and decreased both putrescine stimulated swarming and urothelial cell invasion in a speA mutant.

  19. Development of a Phage Cocktail to Control Proteus mirabilis Catheter-associated Urinary Tract Infections

    Science.gov (United States)

    Melo, Luís D. R.; Veiga, Patrícia; Cerca, Nuno; Kropinski, Andrew M.; Almeida, Carina; Azeredo, Joana; Sillankorva, Sanna

    2016-01-01

    Proteus mirabilis is an enterobacterium that causes catheter-associated urinary tract infections (CAUTIs) due to its ability to colonize and form crystalline biofilms on the catheters surface. CAUTIs are very difficult to treat, since biofilm structures are highly tolerant to antibiotics. Phages have been used widely to control a diversity of bacterial species, however, a limited number of phages for P. mirabilis have been isolated and studied. Here we report the isolation of two novel virulent phages, the podovirus vB_PmiP_5460 and the myovirus vB_PmiM_5461, which are able to target, respectively, 16 of the 26 and all the Proteus strains tested in this study. Both phages have been characterized thoroughly and sequencing data revealed no traces of genes associated with lysogeny. To further evaluate the phages’ ability to prevent catheter’s colonization by Proteus, the phages adherence to silicone surfaces was assessed. Further tests in phage-coated catheters using a dynamic biofilm model simulating CAUTIs, have shown a significant reduction of P. mirabilis biofilm formation up to 168 h of catheterization. These results highlight the potential usefulness of the two isolated phages for the prevention of surface colonization by this bacterium. PMID:27446059

  20. Behaviour of UV-sensitive mutants of Proteus mirabilis to repair incision breaks

    International Nuclear Information System (INIS)

    Stoerl, K.; Mund, C.

    1977-01-01

    In U.V.-sensitive mutants of P. mirabilis with the phenotype HCR, REC and EXR single-strand breaks appeared immediately after UV-irradiation. The behaviour of REC- and EXR-mutants was similar to the wildtype. The number of incision breaks observed by sedimentation analysis in these strains was very low. They could be joined during the excision repair process. From the ability of REC- and EXR-strains to rejoin most of the induced single-strand breaks it can be concluded that these strains have approximately the same capacity for excision repair as the wildtype. HCR-mutants of P. mirabilis produced single-strand breaks after UV-irradiation in contrast to HCR-mutants of E. coli. Therefore we suggest that HCR-mutants of P. mirabilis are not completely inhibited in the incision step. The single-strand breaks introduced in the DNA at the beginning of the repair process were not rejoined during further incubation. Experiments with toluenized cells led to the same results. The newly synthesized daughter DNA-strands of UV-irradiated HCR-mutants were of low molecular weight in comparison with those from unirradiated control cells during the repair period. This result is in agreement with the incapability of HCR-mutants to remove the pyrimidine dimers from the parental template strand. (author)

  1. Characteristics of bacteremia caused by extended-spectrum beta-lactamase-producing Proteus mirabilis.

    Science.gov (United States)

    Kurihara, Yoko; Hitomi, Shigemi; Oishi, Tsuyoshi; Kondo, Tsukasa; Ebihara, Tsugio; Funayama, Yasunori; Kawakami, Yasushi

    2013-10-01

    Although Proteus mirabilis is a common human pathogen, bacteremia caused by the organism, especially strains producing extended-spectrum beta-lactamase (ESBL), has rarely been investigated. We examined 64 cases of P. mirabilis bacteremia identified in the Minami Ibaraki Area, Japan, between 2001 and 2010 and compared the characteristics of cases with ESBL-producing and ESBL-non-producing strains (13 and 51 cases, respectively). All ESBL-producing strains with the gene encoding the CTX-M-2-group were genetically nonidentical. Isolation of ESBL-producing strains was significantly associated with onset in a hospital (p = 0.030), receiving hemodialysis (p = 0.0050), and previous antibiotic use within 1 month (p = 0.036; especially penicillin and/or cephalosporin (p = 0.010) and fluoroquinolone (p = 0.0069)). Isolation was also associated with inappropriate antibiotic therapy on the 1st and 4th days (p = 0.011 and 0.032, respectively) but not with mortality on the 30th day. These findings indicate that, for P. mirabilis bacteremia, isolation of ESBL-producing strains causes delay of initiating appropriate antimicrobial therapy but may not be associated with mortality.

  2. Crust-mantle contribution to Andean magmatism

    International Nuclear Information System (INIS)

    Ruiz, J; Hildreth, W; Chesley, J

    2001-01-01

    There has long been great interest in quantifying the contributions of the continental crust to continental arc magmas, such as those of the Andes using osmium isotopes (Alves et al., 1999; Borg et al., 2000; Brandon et al., 1996; McInnes et al., 1999). In general, Andean volcanic rocks of all compositions show relatively low Sr-isotope ratios and positive to mildly negative epsilon Nd values. Nonetheless, in the Southern Volcanic Zone of central Chile, basalt-andesite-dacite volcanoes along the Quaternary volcanic front were shown (by Hildreth and Moorbath, 1988) to have latitudinally systematic chemical variations, as well as a monotonic increase in 87Sr/Sr86 from ca. 0.7035 to 0.7055 and a decrease in epsilon Nd values from ca. +3 to -1. The isotopic variations correlate with basement elevation of the volcanic edifices and with Bouguer gravity anomalies, both of which are thought to reflect along-arc variations in thickness and average age of the underlying crust. Volcanoes with the most evolved isotopic signatures were fed through the thickest crust. Correlation of chemical and isotopic variations with crustal thickness was interpreted to be caused by Melting (of deep-crustal host rocks), Assimilation, Storage, and Homogenization (MASH) of mantle-derived magmas in long-lived lower-crustal reservoirs beneath each center prior to eruption. We have now determined Os-isotope ratios for a sample suite from these volcanoes (33-36 S lat.), representing a range of crustal thickness from ca. 60-35 km. The samples range in MgO from ca. 8-4% and in SiO2 from 51-57%. The most evolved eruptive products occur above the thickest crust and have 87Sr/86Sr ratios of 0.7054 and epsilon Nd values of -1.5. The 187Os/188Os ratios correlate with the other isotopic systems and with crustal thickness. Volcanoes on the thinnest crust have 187Os/188Os ratios of 0.18-0.21. Those on the thickest crust have 187Os/188Os ratios as high as 0.64. All the Os values are much too radiogenic to

  3. Postmodern Anthropology: Reflections from Andean Ethnohistory

    Directory of Open Access Journals (Sweden)

    Villarías-Robles, Juan J. R.

    2008-06-01

    Full Text Available The postmodern perspective, which began its influence on studies of Prehispanic Peru in the 1980s, has resulted —as chief positive effect— in reflection and debate concerning the written sources for apprehending such cultural otherness, the so-called “Chronicles of the West Indies”: a perspective accompanied by new editions of these texts. The author of the present article expresses his own reflection on such change in theory and method. He argues that, with regard to self-reflectivity on its epistemological foundations, the new perspective is not entirely original in the long history of Andean ethnohistory; in effect, this approach is almost as old as the field itself. What is indeed original is the cognitive relativism that surfaced in some extreme forms of the discussion. It was an unfortunate development, however: when not denying, as a matter of principle, the very possibility of understanding that cultural otherness, arguments masked actual interpretations or explanations of its features that were protected, ipso facto, from a rigorous process of validation.

    La perspectiva posmoderna, que empezó a ser influyente en los estudios del Perú prehispánico en la década de 1980, ha tenido como principal efecto positivo la reflexión y el debate sobre las fuentes originales de conocimiento de esa alteridad cultural, las llamadas genéricamente “Crónicas de Indias”: una perspectiva acompañada de nuevas ediciones de tales textos. El autor del presente artículo hace aquí su propia reflexión sobre este cambio teórico y metodológico. Plantea que, en lo que tiene de discusión sobre sus bases epistemológicas, no es del todo original en la larga historia de la etnohistoria peruanista. Es, de hecho, casi tan antiguo como ella. Lo que sí ha sido original es el relativismo cognitivo que ha acompañado a algunas expresiones extremas de la discusión. Pero fue ésta una novedad desafortunada: cuando no negaba por principio la

  4. Interrill and rill erodibility in the northern andean highlands

    NARCIS (Netherlands)

    Romero, C.; Stroosnijder, L.; Baigorria, G.A.

    2007-01-01

    There is a lack of quantitative information describing the physical processes causing soil erosion in the Andean Highlands, especially those related to interrill and rill erodibility factors. To assess how susceptible are soils to erosion in this region, field measurements of interrill (Ki) and rill

  5. Andean shrublands of Moquegua, South Peru: Prepuna plant communities

    NARCIS (Netherlands)

    Montesinos, D.B.; Cleef, A.M.; Sykora, K.V.

    2012-01-01

    A syntaxonomic overview of shrubland vegetation in the southern Andean regions of Peru is presented. For each plant community, information is given on physiognomy, floristic diversity, ecology and geographical distribution. The shrub vegetation on the slopes of the upper Tambo river valley includes

  6. The Andean Common Market: An Experiment in Regional Cooperation.

    Science.gov (United States)

    Carlson, Reynold E.

    The Grupo Andino (GRAN) was formed in 1969 as an effort at economic integration by six Latin American countries (Bolivia, Chile, Columbia, Ecuador, Peru, and Venezuela). It was an outgrowth of its predecessor, the Latin American Free Trade Association (LAFTA), which had been formed in 1960 with eleven member countries. The Andean Group (GRAN) from…

  7. New environmentally-friendly antimicrobials and biocides from Andean and Mexican biodiversity.

    Science.gov (United States)

    Cespedes, Carlos L; Alarcon, Julio; Aqueveque, Pedro M; Lobo, Tatiana; Becerra, Julio; Balbontin, Cristian; Avila, Jose G; Kubo, Isao; Seigler, David S

    2015-10-01

    Persistent application of pesticides often leads to accumulation in the environment and to the development of resistance in various organisms. These chemicals frequently degrade slowly and have the potential to bio-accumulate across the food chain and in top predators. Cancer and neuronal damage at genomic and proteomic levels have been linked to exposure to pesticides in humans. These negative effects encourage search for new sources of biopesticides that are more "environmentally-friendly" to the environment and human health. Many plant or fungal compounds have significant biological activity associated with the presence of secondary metabolites. Plant biotechnology and new molecular methods offer ways to understand regulation and to improve production of secondary metabolites of interest. Naturally occurring crop protection chemicals offer new approaches for pest management by providing new sources of biologically active natural products with biodegradability, low mammalian toxicity and environmentally-friendly qualities. Latin America is one of the world's most biodiverse regions and provide a previously unsuspected reservoir of new and potentially useful molecules. Phytochemicals from a number of families of plants and fungi from the southern Andes and from Mexico have now been evaluated. Andean basidiomycetes are also a great source of scientifically new compounds that are interesting and potentially useful. Use of biopesticides is an important component of integrated pest management (IPM) and can improve the risks and benefits of production of many crops all over the world. Copyright © 2015 Elsevier Inc. All rights reserved.

  8. Examination of Quaternary Ammonium Compound Resistance in Proteus mirabilis Isolated from Cooked Meat Products in China

    Directory of Open Access Journals (Sweden)

    Xiaobing Jiang

    2017-12-01

    Full Text Available The aim of this study was to examine the presence of genes responsible for resistance to quaternary ammonium compounds (QACs and the association of qac genes with class 1 integrons in Proteus mirabilis isolated from cooked meat products. A total of 52 P. mirabilis isolates (29.2% were detected from 178 samples, and their minimum inhibitory concentrations (MICs of benzalkonium chloride (BC ranged from 4 to >32 μg/mL. The isolates with BC MICs of 24 μg/mL were observed most frequently. PCR assays indicated that mdfA, ydgE/ydgF, qacE, qacEΔ1, emrE, sugE(c, and sugE(p were commonly present (32.7%–100% in these isolates, but qacH was less prevalent (3.8%. Five groups of resistance gene cassettes were identified in 10 intI1-positive isolates. An unusual gene cassette array dfrA32-ereA-aadA2 was found in one foodborne isolate of P. mirabilis. Two isolates harbored qacH- and sul3- associated non-classic integrons: aadA2-cmlA1-aadA1-qacH-IS440-sul3 and a new arrangement dfrA32-ereA1-aadA2-cmlA1-aadA1-qacH-IS440-sul3, which is first reported in P. mirabilis. Non-classic class 1 integrons were located on conjugative plasmids of 100 kb in two tested isolates. Our data showed that the QAC resistance genes were commonly present among P. mirabilis isolates from cooked meats and qacH was associated with non-classic class 1 integrons. The creation of transconjugants demonstrated that qacH-associated non-classic class 1 integrons were located on conjugative plasmids and therefore could facilitate the co-dissemination of disinfectant and antimicrobial resistance genes among bacteria, an increasing area of concern.

  9. Canaryseed Crop

    Directory of Open Access Journals (Sweden)

    Maximiliano Cogliatti

    2012-03-01

    Full Text Available Canaryseed (Phalaris canariensis L. is a graminaceous crop species with production practices and cycle similar to those of other winter cereal crops such as spring wheat (Triticum aestivum L. and oat (Avena sativa L.. Currently its grains are used almost exclusively as feed for birds, alone or mixed with other grains like millet, sunflower seed, and flaxseed. Canaryseed is a genuine cereal with a unique composition that suggests its potential for food use. P. canariensis is cultivated in many areas of temperate climates. Currently, its production is concentrated in the southwestern provinces of Canada (Alberta, Saskatchewan and Manitoba and on a smaller scale in Argentina, Thailand and Australia. Globally it is considered to be a minor crop with regional relevance, with a production about of 250000 tonnes per year, which restricts private investment and public research on its genetic and technological improvement. For this reason, the type of crop management that is applied to this species largely depends on innovations made in other similar crops. This work provides an updated summary of the available information on the species: its requirements, distribution, genetic resources, cultivation practices, potential uses, marketing and other topics of interest to researchers and producers.

  10. Transcriptome of Proteus mirabilis in the Murine Urinary Tract: Virulence and Nitrogen Assimilation Gene Expression▿†

    Science.gov (United States)

    Pearson, Melanie M.; Yep, Alejandra; Smith, Sara N.; Mobley, Harry L. T.

    2011-01-01

    The enteric bacterium Proteus mirabilis is a common cause of complicated urinary tract infections. In this study, microarrays were used to analyze P. mirabilis gene expression in vivo from experimentally infected mice. Urine was collected at 1, 3, and 7 days postinfection, and RNA was isolated from bacteria in the urine for transcriptional analysis. Across nine microarrays, 471 genes were upregulated and 82 were downregulated in vivo compared to in vitro broth culture. Genes upregulated in vivo encoded mannose-resistant Proteus-like (MR/P) fimbriae, urease, iron uptake systems, amino acid and peptide transporters, pyruvate metabolism enzymes, and a portion of the tricarboxylic acid (TCA) cycle enzymes. Flagella were downregulated. Ammonia assimilation gene glnA (glutamine synthetase) was repressed in vivo, while gdhA (glutamate dehydrogenase) was upregulated in vivo. Contrary to our expectations, ammonia availability due to urease activity in P. mirabilis did not drive this gene expression. A gdhA mutant was growth deficient in minimal medium with citrate as the sole carbon source, and loss of gdhA resulted in a significant fitness defect in the mouse model of urinary tract infection. Unlike Escherichia coli, which represses gdhA and upregulates glnA in vivo and cannot utilize citrate, the data suggest that P. mirabilis uses glutamate dehydrogenase to monitor carbon-nitrogen balance, and this ability contributes to the pathogenic potential of P. mirabilis in the urinary tract. PMID:21505083

  11. Antimicrobial susceptibilities of Proteus mirabilis: a longitudinal nationwide study from the Taiwan surveillance of antimicrobial resistance (TSAR) program.

    Science.gov (United States)

    Wang, Jann-Tay; Chen, Pei-Chen; Chang, Shan-Chwen; Shiau, Yih-Ru; Wang, Hui-Ying; Lai, Jui-Fen; Huang, I-Wen; Tan, Mei-Chen; Lauderdale, Tsai-Ling Yang

    2014-09-05

    Longitudinal nationwide data on antimicrobial susceptibility in Proteus mirabilis from different sources are rare. The effects of the revised Clinical and Laboratory Standards Institute (CLSI) β-lactam breakpoints on susceptibility rates and on detecting extended-spectrum β-lactamase (ESBL) and AmpC β-lactamase-producers in this species are also seldom evaluated. The present study analyzed data from the Taiwan Surveillance of Antimicrobial Resistance program to address these issues. Isolates were collected biennially between 2002 and 2012 from 25 to 28 hospitals in Taiwan. Minimum inhibitory concentrations (MIC) were determined by reference broth microdilution method. All isolates with aztreonam, ceftazidime, or cefotaxime MIC ≥ 2 mg/L were checked for the presence of ESBL by CLSI confirmatory test and subjected to ESBL and AmpC β-lactamases gene detection by PCR. Univariate and multivariate analyses were performed. Between 2002 and 2012, a total of 1157 P. mirabilis were studied. Susceptibility to cefotaxime, ceftazidime, and ciprofloxacin decreased significantly during the past decade, from 92.6% to 81.7%, 100% to 95.2%, and 80.1% to 53.8%, respectively (P mirabilis from Taiwan in the past decade. The prevalence of ESBL remained stable but AmpC β-lactamase-producing P. mirabilis increased significantly. Cefotaxime was a better surrogate than ceftazidime for predicting the presence of these β-lactamases. Continuous surveillance on antimicrobial resistance and associated resistance mechanisms in P. mirabilis is warranted.

  12. First Isolation of carbon dioxide-dependent Proteus mirabilis from an uncomplicated cystitis patient with Sjögren's syndrome.

    Science.gov (United States)

    Oana, Kozue; Yamaguchi, Michiko; Nagata, Mika; Washino, Kei-Ichi; Akahane, Takayuki; Takamatsu, Yu-Uki; Tsutsui, Chie; Matsumoto, Takehisa; Kawakami, Yoshiyuki

    2013-01-01

    An uncomplicated cystitis caused by CO2-dependent Proteus mirabilis was observed in a 64-year-old Japanese female patient with Sjögren's syndrome in the Aomori Kyoritsu Hospital, Aomori, Japan. The initial P. mirabilis isolate came from a midstream urine specimen containing large numbers of Gram-negative, rod-shaped organisms that failed to grow on both Drigalski agar and sheep blood agar incubated in ambient air. The organism did grow when the urine was cultured overnight on blood agar under anaerobic conditions. Hence, we believed that the organism was an anaerobe. Further investigation revealed that the isolate grew on sheep blood agar along with swarming when the atmospheric CO2 concentrations were increased to 5%. Initially, we failed to characterize or identify the P. mirabilis isolate or determine its antimicrobial susceptibilities using the MicroScan WalkAway-40 System because the isolate did not grow in the system. However, the isolate was subsequently identified as P. mirabilis based on its morphological, cultural, and biochemical properties by using the commercially available kit systems, Quick ID-GN and ID-Test EB-20. This identification of the isolate was confirmed by sequencing the 16S rRNA gene of the organism. To our knowledge, this is the first clinical isolation of capnophilic P. mirabilis.

  13. Genetic Analysis of Flooding Tolerance in an Andean Diversity Panel of Dry Bean (Phaseolus vulgaris L.

    Directory of Open Access Journals (Sweden)

    Ali Soltani

    2018-06-01

    Full Text Available Climate change models predict temporal and spatial shifts in precipitation resulting in more frequent incidents of flooding, particularly in regions with poor soil drainage. In these flooding conditions, crop losses are inevitable due to exposure of plants to hypoxia and the spread of root rot diseases. Improving the tolerance of bean cultivars to flooding is crucial to minimize crop losses. In this experiment, we evaluated the phenotypic responses of 277 genotypes from the Andean Diversity Panel to flooding at germination and seedling stages. A randomized complete block design, with a split plot arrangement, was employed for phenotyping germination rate, total weight, shoot weight, root weight, hypocotyl length, SPAD index, adventitious root rate, and survival score. A subset of genotypes (n = 20 were further evaluated under field conditions to assess correlations between field and greenhouse data and to identify the most tolerant genotypes. A genome-wide association study (GWAS was performed using ~203 K SNP markers to understand the genetic architecture of flooding tolerance in this panel. Survival scores between field and greenhouse data were significantly correlated (r = 0.55, P = 0.01. Subsequently, a subset of the most tolerant and susceptible genotypes were evaluated under pathogenic Pythium spp. pressure. This experiment revealed a potential link between flooding tolerance and Pythium spp. resistance. Several tolerant genotypes were identified that could be used as donor parents in breeding pipelines, especially ADP-429 and ADP-604. Based on the population structure analysis, a subpopulation consisting of 20 genotypes from the Middle American gene pool was detected that also possessed the highest root weight, hypocotyl length, and adventitious root development under flooding conditions. Genomic regions associated with flooding tolerance were identified including a region on Pv08/3.2 Mb, which is associated with germination rate and

  14. Phenotypic and molecular characterization of antimicrobial resistance in Proteus mirabilis isolates from dogs.

    Science.gov (United States)

    Harada, Kazuki; Niina, Ayaka; Shimizu, Takae; Mukai, Yujiro; Kuwajima, Ken; Miyamoto, Tadashi; Kataoka, Yasushi

    2014-11-01

    Large-scale monitoring of resistance to 14 antimicrobial agents was performed using 103 Proteus mirabilis strains isolated from dogs in Japan. Resistant strains were analysed to identify their resistance mechanisms. Rates of resistance to chloramphenicol, streptomycin, enrofloxacin, trimethoprim/sulfamethoxazole, kanamycin, ampicillin, ciprofloxacin, cephalothin, gentamicin, cefoxitin and cefotaxime were 20.4, 15.5, 12.6, 10.7, 9.7, 8.7, 5.8, 2.9, 2.9, 1.9 and 1.9%, respectively. No resistance to ceftazidime, aztreonam or imipenem was found. Class 1 and 2 integrases were detected in 2.9 and 11.7% of isolates, respectively. Class 1 integrons contained aadB or aadB-catB-like-blaOXA10-aadA1, whereas those of class 2 contained sat-aadA1, dhfr1-sat-aadA1 or none of the anticipated resistance genes. Of five distinct plasmid-mediated quinolone-resistance (PMQR) genes, only qnrD gene was detected in 1.9% of isolates. Quinolone-resistance determining regions (QRDRs) of gyrA and parC from 13 enrofloxacin-intermediate and -resistant isolates were sequenced. Seven strains had double mutations and three had single mutations. Three of nine ampicillin-resistant isolates harboured AmpC-type β-lactamases (i.e. blaCMY-2, blaCMY-4 and blaDHA-1). These results suggest that canine Proteus mirabilis deserves continued surveillance as an important reservoir of antimicrobial resistance determinants. This is the first report, to our knowledge, describing integrons, PMQRs and QRDR mutations in Proteus mirabilis isolates from companion animals. © 2014 The Authors.

  15. Proteus vulgaris and Proteus mirabilis Decrease Candida albicans Biofilm Formation by Suppressing Morphological Transition to Its Hyphal Form.

    Science.gov (United States)

    Lee, Kyoung Ho; Park, Su Jung; Choi, Sun Ju; Park, Joo Young

    2017-11-01

    Candida albicans (C. albicans) and Proteus species are causative agents in a variety of opportunistic nosocomial infections, and their ability to form biofilms is known to be a virulence factor. In this study, the influence of co-cultivation with Proteus vulgaris (P. vulgaris) and Proteus mirabilis (P. mirabilis) on C. albicans biofilm formation and its underlying mechanisms were examined. XTT reduction assays were adopted to measure biofilm formation, and viable colony counts were performed to quantify yeast growth. Real-time reverse transcriptase polymerase chain reaction was used to evaluate the expression of yeast-specific genes (rhd1 and rbe1), filament formation inhibiting genes (tup1 and nrg1), and hyphae-related genes (als3, ece1, hwp1, and sap5). Candida biofilm formation was markedly inhibited by treatment with either living or heat-killed P. vulgaris and P. mirabilis. Proteus-cultured supernatant also inhibited Candida biofilm formation. Likewise, treatment with live P. vulgaris or P. mirabilis or with Proteus-cultured supernatant decreased expression of hyphae-related C. albicans genes, while the expression of yeast-specific genes and the filament formation inhibiting genes of C. albicans were increased. Heat-killed P. vulgaris and P. mirabilis treatment, however, did not affect the expression of C. albicans morphology-related genes. These results suggest that secretory products from P. vulgaris and P. mirabilis regulate the expression of genes related to morphologic changes in C. albicans such that transition from the yeast form to the hyphal form can be inhibited. © Copyright: Yonsei University College of Medicine 2017

  16. Cranberry derivatives enhance biofilm formation and transiently impair swarming motility of the uropathogen Proteus mirabilis HI4320.

    Science.gov (United States)

    O'May, Che; Amzallag, Olivier; Bechir, Karim; Tufenkji, Nathalie

    2016-06-01

    Proteus mirabilis is a major cause of catheter-associated urinary tract infection (CAUTI), emphasizing that novel strategies for targeting this bacterium are needed. Potential targets are P. mirabilis surface-associated swarming motility and the propensity of these bacteria to form biofilms that may lead to catheter blockage. We previously showed that the addition of cranberry powder (CP) to lysogeny broth (LB) medium resulted in impaired P. mirabilis swarming motility over short time periods (up to 16 h). Herein, we significantly expanded on those findings by exploring (i) the effects of cranberry derivatives on biofilm formation of P. mirabilis, (ii) whether swarming inhibition occurred transiently or over longer periods more relevant to real infections (∼3 days), (iii) whether swarming was also blocked by commercially available cranberry juices, (iv) whether CP or cranberry juices exhibited effects under natural urine conditions, and (v) the effects of cranberry on medium pH, which is an indirect indicator of urease activity. At short time scales (24 h), CP and commercially available pure cranberry juice impaired swarming motility and repelled actively swarming bacteria in LB medium. Over longer time periods more representative of infections (∼3 days), the capacity of the cranberry material to impair swarming diminished and bacteria would start to migrate across the surface, albeit by exhibiting a different motility phenotype to the regular "bull's-eye" swarming phenotype of P. mirabilis. This bacterium did not swarm on urine agar or LB agar supplemented with urea, suggesting that any potential application of anti-swarming compounds may be better suited to settings external to the urine environment. Anti-swarming effects were confounded by the ability of cranberry products to enhance biofilm formation in both LB and urine conditions. These findings provide key insights into the long-term strategy of targeting P. mirabilis CAUTIs.

  17. In vitro anti-inflammatory components isolated from the carnivorous plant Nepenthes mirabilis (Lour.) Rafarin.

    Science.gov (United States)

    Thao, Nguyen Phuong; Luyen, Bui Thi Thuy; Koo, Jung Eun; Kim, Sohyun; Koh, Young Sang; Thanh, Nguyen Van; Cuong, Nguyen Xuan; Kiem, Phan Van; Minh, Chau Van; Kim, Young Ho

    2016-01-01

    Nepenthes mirabilis (Lour.) Rafarin (Nepenthaceae) is a carnivorous plant used as a folk medicine in the treatment of jaundice, hepatitis, gastric ulcers, ureteral stones, diarrhea, diabetes, and high blood pressure. Neither the phytochemical content nor biological activities of N. mirabilis have been reported. The anti-inflammatory activity from the N. mirabilis methanolic extract led to the isolation of compounds (1-26). Chromatographic methods were used to isolate compounds from the methanol extract of N. mirabilis branches and leaves. The anti-inflammatory activity of these isolated compounds was investigated in lipopolysaccharide (LPS)-stimulated bone marrow-derived dendritic cells (BMDCs) using ELISA. Primary BMDCs were used to examine the production of pro-inflammatory cytokines (IL-12 p40, IL-6, and TNF-α, at concentrations of 0.1, 0.2, and 1.0 μM) as compared with a positive control, SB203580 (1.0 μM). MTT assays showed that isolated compounds (1-26) did not exhibit significant cytotoxicity at concentrations up to 20.0 μM. Compound 9 showed potent inhibition of IL-12 p40, IL-6, and TNF-α production (IC50 = 0.17 ± 0.02, 0.46 ± 0.01, and 8.28 ± 0.21 μM, respectively). Compound 4 showed potent inhibition of IL-12 p40 and IL-6 production (IC50 = 1.17 ± 0.01 and 2.15 ± 0.04 μM). In addition, IL-12 p40 inhibition by naphthalene derivatives (1-7, 9, and 10), phenolic compounds (11-15), lupeone (18), and flavonoids (22, 25, and 26) was more potent than with the positive control. The isolated compounds exhibited little and/or no inhibitory effects on TNF-α production in LPS-stimulated BMDCs. Taken together, these data suggest that the isolated components have significant inhibitory effects on pro-inflammatory cytokine production and warrant further study concerning their potential medicinal use.

  18. Overexpression of an Outer Membrane Protein Associated with Decreased Susceptibility to Carbapenems in Proteus mirabilis

    Science.gov (United States)

    Tsai, Yi-Lin; Wang, Min-Cheng; Hsueh, Po-Ren; Liu, Ming-Che; Hu, Rouh-Mei; Wu, Yue-Jin; Liaw, Shwu-Jen

    2015-01-01

    Proteus mirabilis isolates commonly have decreased susceptibility to imipenem. Previously, we found P. mirabilis hfq mutant was more resistant to imipenem and an outer membrane protein (OMP) could be involved. Therefore, we investigated the role of this OMP in carbapenem susceptibility. By SDS-PAGE we found this OMP (named ImpR) was increased in hfq mutant and LC-MS/MS revealed it to be the homologue of Salmonella YbfM, which is a porin for chitobiose and subject to MicM (a small RNA) regulation. We demonstrated that ImpR overexpression resulted in increased carbapenem MICs in the laboratory strain and clinical isolates. Chitobiose induced expression of chb (a chitobiose utilization operon). Real-time RT-PCR and SDS-PAGE were performed to elucidate the relationship of hfq, impR, chb and MicM in P. mirabilis. We found MicM RNA was decreased in hfq mutant and chbBC-intergenic region (chbBC-IGR) overexpression strain (chbIGRov), while impR mRNA was increased in hfq mutant, micM mutant and chbIGRov strain. In addition, mutation of hfq or micM and overexpression of chbBC-IGR increased ImpR protein level. Accordingly, chitobiose made wild-type have higher levels of ImpR protein and are more resistant to carbapenems. Hfq- and MicM-complemented strains restored wild-type MICs. Mutation of both impR and hfq eliminated the increase in carbapenem MICs observed in hfq mutant and ImpR-complementation of hfq/impR double mutant resulted in MICs as hfq mutant, indicating that the ImpR-dependent decreased carbapenem susceptibility of hfq mutant. These indicate MicM was antisense to impR mRNA and was negatively-regulated by chbBC-IGR. Together, overexpression of ImpR contributed to the decreased carbapenem susceptibility in P. mirabilis. PMID:25756370

  19. Assessment of Cadmium and Chromium Stress on Growth, Physiology and Metal Uptake Using Mirabilis Jalapa

    OpenAIRE

    S. A. Shahanaz Begum; Tharakeswar Yadamari; Kalyan Yakkala; Sreevani Parvathareddy; Ramakrishna Naidu Gurijala

    2015-01-01

    Phytoextraction potential of Mirabilis jalapa, with tuberous root having high ecological adoptability was studied in the present work . Different levels of cadmium and chromium stress on growth, physiology and metal uptake were studied using pot experiments. The experiment comprised of 5 dosages of cadmium and chromium with different test concentrations (TC) viz, TC1(0), TC2(25), TC3(50), TC4(75) and TC5(100) ppm, for the period of 45 days. Growth, physiological parameters and metal accumulat...

  20. Elimination of Proteus mirabilis /sup 51/Cr endotoxin from the liver in rats

    Energy Technology Data Exchange (ETDEWEB)

    Lipinska-Piotrowska, I [Akademia Medyczna, Lodz (Poland)

    1977-01-01

    Using isotope methods, elimination of the endotoxin of Proteus mirabilis labelled with chromium (CrEPm) from the liver of rats was studied. The following studies were carried out: intravital exploration of the liver with a scintillation probe, measurements of radioactivity of organs and excreted urine and stools, scintigraphy of the liver, binding of CrEPm by subcellular fractions of hepatocytes, and the influence of selected drugs (polymyxin and hydrocortisone) on elimination of CrEPm from the liver and organelles of hepatocytes.

  1. Excision repair in MUT-mutants of Proteus mirabilis after UV-irradiation

    International Nuclear Information System (INIS)

    Stoerl, K.; Mund, C.

    1977-01-01

    The behaviour of MUT-mutants of P.mirabilis to perform certain steps of excision repair after U.V.-irradiation is described. MUT-mutants introduce single-strand breaks in the DNA immediately after U.V.-irradiation, but their ability to excise pyrimidine dimers from the DNA is very diminished. Moreover, they are not able to accomplish the excision repair by rejoining of the single-strand breaks. The connection between the incomplete excision repair and the mutator phenotype of these mutants is discussed. (author)

  2. Regulation of the Min Cell Division Inhibition Complex by the Rcs Phosphorelay in Proteus mirabilis.

    Science.gov (United States)

    Howery, Kristen E; Clemmer, Katy M; Şimşek, Emrah; Kim, Minsu; Rather, Philip N

    2015-08-01

    A key regulator of swarming in Proteus mirabilis is the Rcs phosphorelay, which represses flhDC, encoding the master flagellar regulator FlhD4C2. Mutants in rcsB, the response regulator in the Rcs phosphorelay, hyperswarm on solid agar and differentiate into swarmer cells in liquid, demonstrating that this system also influences the expression of genes central to differentiation. To gain a further understanding of RcsB-regulated genes involved in swarmer cell differentiation, transcriptome sequencing (RNA-Seq) was used to examine the RcsB regulon. Among the 133 genes identified, minC and minD, encoding cell division inhibitors, were identified as RcsB-activated genes. A third gene, minE, was shown to be part of an operon with minCD. To examine minCDE regulation, the min promoter was identified by 5' rapid amplification of cDNA ends (5'-RACE), and both transcriptional lacZ fusions and quantitative real-time reverse transcriptase (qRT) PCR were used to confirm that the minCDE operon was RcsB activated. Purified RcsB was capable of directly binding the minC promoter region. To determine the role of RcsB-mediated activation of minCDE in swarmer cell differentiation, a polar minC mutation was constructed. This mutant formed minicells during growth in liquid, produced shortened swarmer cells during differentiation, and exhibited decreased swarming motility. This work describes the regulation and role of the MinCDE cell division system in P. mirabilis swarming and swarmer cell elongation. Prior to this study, the mechanisms that inhibit cell division and allow swarmer cell elongation were unknown. In addition, this work outlines for the first time the RcsB regulon in P. mirabilis. Taken together, the data presented in this study begin to address how P. mirabilis elongates upon contact with a solid surface. Copyright © 2015, American Society for Microbiology. All Rights Reserved.

  3. Computational study concerning the effect of some pesticides on the Proteus Mirabilis catalase activity

    Science.gov (United States)

    Isvoran, Adriana

    2016-03-01

    Assessment of the effects of the herbicides nicosulfuron and chlorsulfuron and the fungicides difenoconazole and drazoxlone upon catalase produced by soil microorganism Proteus mirabilis is performed using the molecular docking technique. The interactions of pesticides with the enzymes are predicted using SwissDock and PatchDock docking tools. There are correlations for predicted binding energy values for enzyme-pesticide complexes obtained using the two docking tools, all the considered pesticides revealing favorable binding to the enzyme, but only the herbicides bind to the catalytic site. These results suggest the inhibitory potential of chlorsulfuron and nicosulfuron on the catalase activity in soil.

  4. Molecular characteristics of Salmonella genomic island 1 in Proteus mirabilis isolates from poultry farms in China.

    Science.gov (United States)

    Lei, Chang-Wei; Zhang, An-Yun; Liu, Bi-Hui; Wang, Hong-Ning; Guan, Zhong-Bin; Xu, Chang-Wen; Xia, Qing-Qing; Cheng, Han; Zhang, Dong-Dong

    2014-12-01

    Six out of the 64 studied Proteus mirabilis isolates from 11 poultry farms in China contained Salmonella genomic island 1 (SGI1). PCR mapping showed that the complete nucleotide sequences of SGI1s ranged from 33.2 to 42.5 kb. Three novel variants, SGI1-W, SGI1-X, and SGI1-Y, have been characterized. Resistance genes lnuF, dfrA25, and qnrB2 were identified in SGI1 for the first time. Copyright © 2014, American Society for Microbiology. All Rights Reserved.

  5. Elimination of Proteus mirabilis 51Cr endotoxin from the liver in rats

    International Nuclear Information System (INIS)

    Lipinska-Piotrowska, I.

    1977-01-01

    Using isotope methods, elimination of the endotoxin of Proteus mirabilis labelled with chromium (CrEPm) from the liver of rats was studied. The following studies were carried out: intravital exploration of the liver with a scintillation probe, measurements of radioactivity of organs and excreted urine and stools, scintigraphy of the liver, binding of CrEPm by subcellular fractions of hepatocytes, and the influence of selected drugs (polymyxin and hydrocortisone) on elimination of CrEPm from the liver and organelles of hepatocytes. (author)

  6. TEM-187, a new extended-spectrum β-lactamase with weak activity in a Proteus mirabilis clinical strain.

    Science.gov (United States)

    Corvec, Stéphane; Beyrouthy, Racha; Crémet, Lise; Aubin, Guillaume Ghislain; Robin, Frédéric; Bonnet, Richard; Reynaud, Alain

    2013-05-01

    A Proteus mirabilis clinical strain (7001324) was isolated from urine sample of a patient hospitalized in a long-term-care facility. PCR and cloning experiments performed with this strain identified a novel TEM-type β-lactamase (TEM-187) differing by four amino acid substitutions (Leu21Phe, Arg164His, Ala184Val, and Thr265Met) from TEM-1. This characterization provides further evidence for the diversity of extended-spectrum β-lactamases (ESBL) produced by P. mirabilis and for their potential spread to other Enterobacteriaceae due to a lack of sensitive detection methods used in daily practice.

  7. Social inequality and child malnutrition in four Andean countries

    OpenAIRE

    Carlos Larrea; Wilma Freire

    2002-01-01

    Objective. To analyze the effects of socioeconomic, regional, and ethnic conditions on chronic malnutrition in four Andean countries of South America: Bolivia, Colombia, Ecuador, and Peru. Methods. The study was based on Demographic and Health Surveys (DHS) for Colombia (1995), Peru (1996), and Bolivia (1997), and on a Living Standard Measurement Survey for Ecuador (1998). We developed an index of household socioeconomic status using categorical principal components analysis. We broke down th...

  8. Predicting Polylepis distribution: vulnerable and increasingly important Andean woodlands

    Directory of Open Access Journals (Sweden)

    Brian R. Zutta

    2012-11-01

    Full Text Available Polylepis woodlands are a vital resource for preserving biodiversity and hydrological functions, which will be altered by climate change and challenge the sustainability of local human communities. However, these highaltitude Andean ecosystems are becoming increasingly vulnerable due to anthropogenic pressure including fragmentation, deforestation and the increase in livestock. Predicting the distribution of native woodlands has become increasingly important to counteract the negative effects of climate change through reforestation and conservation. The objective of this study was to develop and analyze the distribution models of two species that form extensive woodlands along the Andes, namely Polylepis sericea and P. weberbaueri. This study utilized the program Maxent, climate and remotely sensed environmental layers at 1 km resolution. The predicted distribution model for P. sericea indicated that the species could be located in a variety of habitats along the Andean Cordillera, while P. weberbaueri was restricted to the high elevations of southern Peru and Bolivia. For both species, elevation and temperature metrics were the most significant factors for predicted distribution. Further model refinement of Polylepis and other Andean species using increasingly available satellite data demonstrate the potential to help define areas of diversity and improve conservation strategies for the Andes.

  9. Andean cutaneous leishmaniasis (Andean-CL, uta) in Peru and Ecuador: the causative Leishmania parasites and clinico-epidemiological features.

    Science.gov (United States)

    Hashiguchi, Yoshihisa; Gomez, Eduardo A L; Cáceres, Abraham G; Velez, Lenin N; Villegas, Nancy V; Hashiguchi, Kazue; Mimori, Tatsuyuki; Uezato, Hiroshi; Kato, Hirotomo

    2018-01-01

    This study provides comprehensive information on the past and current status of the Andean cutaneous leishmaniasis (Andean-CL, uta) in Peru and Ecuador, mainly focusing on the causative Leishmania parasites and clinico-epidemiological features. Available information and data including our unpublished works were analyzed thoroughly. Endemic regions of the Andean-CL (uta) in Peru run from the north Piura/Cajamarca to the south Ayacucho at a wide range of the Pacific watersheds of the Andes through several departments, while in Ecuador those exist at limited and spotted areas in the country's mid-southwestern two provinces, Azuay and Chimborazo. The principal species of the genus Leishmania are completely different at subgenus level, L. (Viannia) peruviana in Peru, and L. (Leishmania) mexicana and L. (L.) major-like (infrequent occurrence) in Ecuador. The Peruvian uta is now prevalent in different age and sex groups, being not clearly defined as found in the past. The precise reasons are not known and should be elucidated further, though probable factors, such as emergence of other Leishmania parasites, non-immune peoples' migration into the areas, etc., were discussed briefly in the text. The Andean-CL cases in Ecuador are more rural than before, probably because of a rapid development of the Leishmania-positive communities and towns, and the change of life-styles of the inhabitants, including newly constructed houses and roads in the endemic areas. Such information is helpful for future management of the disease, not only for Leishmania-endemic areas in the Andes but also for other endemic areas. Copyright © 2017 Elsevier B.V. All rights reserved.

  10. Proteus mirabilis alleviates zinc toxicity by preventing oxidative stress in maize (Zea mays) plants.

    Science.gov (United States)

    Islam, Faisal; Yasmeen, Tahira; Riaz, Muhammad; Arif, Muhammad Saleem; Ali, Shafaqat; Raza, Syed Hammad

    2014-12-01

    Plant-associated bacteria can have beneficial effects on the growth and health of their host. However, the role of plant growth promoting bacteria (PGPR), under metal stress, has not been widely investigated. The present study investigated the possible mandatory role of plant growth promoting rhizobacteria in protecting plants from zinc (Zn) toxicity. The exposure of maize plants to 50µM zinc inhibited biomass production, decreased chlorophyll, total soluble protein and strongly increased accumulation of Zn in both root and shoot. Similarly, Zn enhanced hydrogen peroxide, electrolyte leakage and lipid peroxidation as indicated by malondaldehyde accumulation. Pre-soaking with novel Zn tolerant bacterial strain Proteus mirabilis (ZK1) isolated zinc (Zn) contaminated soil, alleviated the negative effect of Zn on growth and led to a decrease in oxidative injuries caused by Zn. Furthermore, strain ZK1 significantly enhanced the activities of catalase, guaiacol peroxidase, superoxide dismutase and ascorbic acid but lowered the Proline accumulation in Zn stressed plants. The results suggested that the inoculation of Zea mays plants with P. mirabilis during an earlier growth period could be related to its plant growth promoting activities and avoidance of cumulative damage upon exposure to Zn, thus reducing the negative consequences of oxidative stress caused by heavy metal toxicity. Copyright © 2014 Elsevier Inc. All rights reserved.

  11. Secondary metabolites of Mirabilis jalapa structurally inhibit Lactate Dehydrogenase A in silico: a potential cancer treatment

    Science.gov (United States)

    Kusumawati, R.; Nasrullah, A. H.; Pesik, R. N.; Muthmainah; Indarto, D.

    2018-03-01

    Altered energy metabolism from phosphorylated oxidation to aerobic glycolysis is one of the cancer hallmarks. Lactate dehydrogenase A (LDHA) is a major enzyme that catalyses pyruvate to lactate in such condition. The aim of this study was to explore LDHA inhibitors derived from Indonesian herbal plants. In this study, LDHA and oxamate molecular structures were obtained from protein data bank. As a standard ligand inhibitor, oxamate was molecularly re-validated using Autodock Vina 1.1.2 software and showed binding energy -4.26 ± 0.006 kcal/mol and interacted with LDHA at Gln99, Arg105, Asn137, Arg168, His192, and Thr247 residues. Molecular docking was used to visualize interaction between Indonesian phytochemicals and LDHA. Indonesian phytochemicals with the lowest binding energy and similar residues with standard ligand was Miraxanthin-III (-8.53 ± 0.006 kcal/mol), Vulgaxanthin-I (-8.46 ± 0.006 kcal/mol), Miraxanthin-II (-7.9 ± 0.2 kcal/mol) and Miraxanthin-V (-7.96 ± kcal/mol). Lower energy binding to LDHA and binding site at these residues was predicted to inhibit LDHA activity better than standard ligand. All phytochemicals were found in Mirabilis jalapa plant. Secondary metabolites in Mirabilis jalapa have LDHA inhibitor property in silico. Further in vitro study should be performed to confirm this result.

  12. Biomineralization of struvite crystals by Proteus mirabilis from artificial urine and their mesoscopic structure

    Energy Technology Data Exchange (ETDEWEB)

    Prywer, J. [Institute of Physics, Technical University of Lodz, ul. Wolczanska 219, 93-005 Lodz (Poland); Torzewska, A. [Department of Immunobiology of Bacteria, Institute of Microbiology, Biotechnology and Immunology, University of Lodz, ul. Banacha 12/16, 90-237 Lodz (Poland)

    2010-12-15

    In order to investigate the mineralization of struvite we performed the experiment of struvite growth process from artificial urine. The crystallization process was induced by Proteus mirabilis, as these bacteria are mainly isolated from infectious stones. The crystallization process occurred at conditions mimicking the real urinary tract infection. Our results show that struvite exhibits polar properties. This feature of struvite crystals is potentially very important in the case of binding additives which may either enhance or inhibit crystallization process. It seems also that the differences in the polarity of opposite faces of c-axis play important role in directing the struvite mesoscopic arrangement. We also described recent developments concerning curcumin - pigment extracted from the roots of turmeric commonly known as a spice added to various food preparations. Curcumin exhibited the effect against Proteus mirabilis inhibiting the activity of urease and consequently decreasing the efficiency of struvite growth. Therefore, curcumin belongs to phytoterapheutic components, which may be the alternative with relation to the antibiotic therapy. The paper concludes with a future outlook and goals in this field of research. (copyright 2010 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim) (orig.)

  13. The Rcs regulon in Proteus mirabilis: implications for motility, biofilm formation, and virulence.

    Science.gov (United States)

    Howery, Kristen E; Clemmer, Katy M; Rather, Philip N

    2016-11-01

    The overall role of the Rcs phosphorelay in Proteus mirabilis is largely unknown. Previous work had demonstrated that the Rcs phosphorelay represses the flhDC operon and activates the minCDE cell division inhibition system. To identify additional cellular functions regulated by the Rcs phosphorelay, an analysis of RNA-seq data was undertaken. In this report, the results of the RNA-sequencing are discussed with an emphasis on the predicted roles of the Rcs phosphorelay in swarmer cell differentiation, motility, biofilm formation, and virulence. RcsB is shown to activate genes important for differentiation and fimbriae formation, while repressing the expression of genes important for motility and virulence. Additionally, to follow up on the RNA-Seq data, we demonstrate that an rcsB mutant is deficient in its ability to form biofilm and exhibits enhanced virulence in a Galleria mellonella waxworm model. Overall, these results indicate the Rcs regulon in P. mirabilis extends beyond flagellar genes to include those involved in biofilm formation and virulence. Furthermore, the information presented in this study may provide clues to additional roles of the Rcs phosphorelay in other members of the Enterobacteriaceae.

  14. The role of Proteus mirabilis cell wall features in biofilm formation.

    Science.gov (United States)

    Czerwonka, Grzegorz; Guzy, Anna; Kałuża, Klaudia; Grosicka, Michalina; Dańczuk, Magdalena; Lechowicz, Łukasz; Gmiter, Dawid; Kowalczyk, Paweł; Kaca, Wiesław

    2016-11-01

    Biofilms formed by Proteus mirabilis strains are a serious medical problem, especially in the case of urinary tract infections. Early stages of biofilm formation, such as reversible and irreversible adhesion, are essential for bacteria to form biofilm and avoid eradication by antibiotic therapy. Adhesion to solid surfaces is a complex process where numerous factors play a role, where hydrophobic and electrostatic interactions with solid surface seem to be substantial. Cell surface hydrophobicity and electrokinetic potential of bacterial cells depend on their surface composition and structure, where lipopolysaccharide, in Gram-negative bacteria, is prevailing. Our studies focused on clinical and laboratory P. mirabilis strains, where laboratory strains have determined LPS structures. Adherence and biofilm formation tests revealed significant differences between strains adhered in early stages of biofilm formation. Amounts of formed biofilm were expressed by the absorption of crystal violet. Higher biofilm amounts were formed by the strains with more negative values of zeta potential. In contrast, high cell surface hydrophobicity correlated with low biofilm amount.

  15. The Ciprofloxacin Impact on Biofilm Formation by Proteus Mirabilis and P. Vulgaris Strains

    Science.gov (United States)

    Kwiecinska-Pirog, Joanna; Skowron, Krzysztof; Bartczak, Wojciech; Gospodarek-Komkowska, Eugenia

    2016-01-01

    Background Proteus spp. bacilli belong to opportunistic human pathogens, which are primarily responsible for urinary tract and wound infections. An important virulence factor is their ability to form biofilms that greatly reduce the effectiveness of antibiotics in the site of infection. Objectives The aim of this study was to determine the value of the minimum concentration of ciprofloxacin that eradicates a biofilm of Proteus spp. strains. Materials and Methods A biofilm formation of 20 strains of P. mirabilis and 20 strains of P. vulgaris were evaluated by a spectrophotometric method using 0.1% 2, 3, 5-Triphenyl-tetrazolium chloride solution (TTC, AVANTORTM). On the basis of the results of the absorbance of the formazan, a degree of reduction of biofilm and minimum biofilm eradication (MBE) values of MBE50 and MBE90 were determined. Results All tested strains formed a biofilm. A value of 1.0 μg/mL ciprofloxacin is MBE50 for the strains of both tested species. An MBE90 value of ciprofloxacin for isolates of P. vulgaris was 2 μg/mL and for P. mirabilis was 512 μg/mL. Conclusions Minimum biofilm eradication values of ciprofloxacin obtained in the study are close to the values of the minimal inhibition concentration (MIC). PMID:27303616

  16. Andean contributions to the biogeochemistry of the amazon river system

    Directory of Open Access Journals (Sweden)

    1995-01-01

    Atlántico. Un nuevo programa colaborativo de investigación se inició en 1994 con el propósito de caracterizar de una manera más completa la biogeoquímica de los ríos andinos. Contributions from Andean rivers may play a significant role in determining the basin-wide biogeochemistry integrated into the mainstem Amazon River of Brazil. Concentration data for organic C, NO3-, and PO43- in Andean rivers are highly variable and reveal no clear spatial or altitudinal patterns. Concentrations measured in Andean rivers are similar to those reported in the mainstem Amazon river and its major tributaries. Explanations of processes which alter Andean-derived particulates and solutes as they exit the Cordillera are only speculative at this time, but their net effect is to diminish Andean signals through decomposition and dilution by lowland inputs. The 13C of particulate and dissolved organic matter in the mainstem Amazon provides evidence that some fraction of Andean derived material persists within the river system, ultimately to be discharged to the Atlantic Ocean. In 1994 a new collaborative research program was launched to further characterize the biogeochemistry of Andean rivers.

  17. Native flagellin does not protect mice against an experimental Proteus mirabilis ascending urinary tract infection and neutralizes the protective effect of MrpA fimbrial protein.

    Science.gov (United States)

    Scavone, Paola; Umpiérrez, Ana; Rial, Analía; Chabalgoity, José A; Zunino, Pablo

    2014-06-01

    Proteus mirabilis expresses several virulence factors including MR/P fimbriae and flagella. Bacterial flagellin has frequently shown interesting adjuvant and protective properties in vaccine formulations. However, native P. mirabilis flagellin has not been analyzed so far. Native P. mirabilis flagellin was evaluated as a protective antigen and as an adjuvant in co-immunizations with MrpA (structural subunit of MR/P fimbriae) using an ascending UTI model in the mouse. Four groups of mice were intranasally treated with either MrpA, native flagellin, both proteins and PBS. Urine and blood samples were collected before and after immunization for specific antibodies determination. Cytokine production was assessed in immunized mice splenocytes cultures. Mice were challenged with P. mirabilis, and bacteria quantified in kidneys and bladders. MrpA immunization induced serum and urine specific anti-MrpA antibodies while MrpA coadministered with native flagellin did not. None of the animals developed significant anti-flagellin antibodies. Only MrpA-immunized mice showed a significant decrease of P. mirabilis in bladders and kidneys. Instead, infection levels in MrpA-flagellin or flagellin-treated mice showed no significant differences with the control group. IL-10 was significantly induced in splenocytes of mice that received native flagellin or MrpA-flagellin. Native P. mirabilis flagellin did not protect mice against an ascending UTI. Moreover, it showed an immunomodulatory effect, neutralizing the protective role of MrpA. P. mirabilis flagellin exhibits particular immunological properties compared to other bacterial flagellins.

  18. Increased Incidence of Urolithiasis and Bacteremia During Proteus mirabilis and Providencia stuartii Coinfection Due to Synergistic Induction of Urease Activity

    Science.gov (United States)

    Armbruster, Chelsie E.; Smith, Sara N.; Yep, Alejandra; Mobley, Harry L. T.

    2014-01-01

    Background. Catheter-associated urinary tract infections (CaUTIs) are the most common hospital-acquired infections worldwide and are frequently polymicrobial. The urease-positive species Proteus mirabilis and Providencia stuartii are two of the leading causes of CaUTIs and commonly co-colonize catheters. These species can also cause urolithiasis and bacteremia. However, the impact of coinfection on these complications has never been addressed experimentally. Methods. A mouse model of ascending UTI was utilized to determine the impact of coinfection on colonization, urolithiasis, and bacteremia. Mice were infected with P. mirabilis or a urease mutant, P. stuartii, or a combination of these organisms. In vitro experiments were conducted to assess growth dynamics and impact of co-culture on urease activity. Results. Coinfection resulted in a bacterial load similar to monospecies infection but with increased incidence of urolithiasis and bacteremia. These complications were urease-dependent as they were not observed during coinfection with a P. mirabilis urease mutant. Furthermore, total urease activity was increased during co-culture. Conclusions. We conclude that P. mirabilis and P. stuartii coinfection promotes urolithiasis and bacteremia in a urease-dependent manner, at least in part through synergistic induction of urease activity. These data provide a possible explanation for the high incidence of bacteremia resulting from polymicrobial CaUTI. PMID:24280366

  19. Why do males of the spider Pisaura mirabilis wrap their nuptial gifts in silk: Female preference or male control?

    DEFF Research Database (Denmark)

    Andersen, Tina; Bollerup, Kristine; Toft, Søren

    2008-01-01

    Males of the spider Pisaura mirabilis present a nuptial prey gift to the female during courtship as a mating effort. The gift is usually round and wrapped in white silk. It was suggested that the wrapped gift functions as a sensory trap by mimicking the female's eggsac implying that males exploit...

  20. No preference for novel mating partners in the polyandrous nuptial-feeding spider Pisaura mirabilis (Araneae: Pisauridae)

    DEFF Research Database (Denmark)

    Tuni, Cristina; Bilde, T.

    2010-01-01

    for novel partners is also expected to maximize male lifetime reproductive success by allowing males to increase the number of mates. We investigated male and female preference for novel or former mating partners in the spider Pisaura mirabilis by offering females novel males (polyandry) or the same male...

  1. Inhibitory effects of secondary metabolites from the red alga Delisea pulchra on swarming motility of Proteus mirabilis

    DEFF Research Database (Denmark)

    Gram, Lone; de Nys, R.; Maximilien, R.

    1996-01-01

    Abnormal, uncoordinated swarming motility of the opportunistic human pathogen Proteus mirabilis was seen when a crude extract of the Australian red alga Delisea pulchra was added to the medium, This occurred at concentrations at which growth rate, swimming motility, cell elongation, polynucleation...

  2. Analisis Vegetasi dalam Komunitas Nepenthes mirabilis di Hutan Kampus Institut Agama Islam Negeri Sultan Thaha Saifuddin Jambi

    Directory of Open Access Journals (Sweden)

    Try Susanti

    2016-01-01

    Full Text Available Abstrak. Nepenthes merupakan tumbuhan unik yaitu mempunyai kemampuan untuk mencerna serangga yang terperangkap dalam organ berbentuk kantong pada ujung daun untuk memenuhi kebutuhan proteinnya. Nephentes dapat dijadikan sebagai tanaman hias yang memiliki nilai komersial tinggi dan juga sebagai obat-obatan. Sumatera merupakan wilayah terbesar kedua dari penyebaran Nepenthes setelah Kalimantan. Habitat alami dari jenis Nepenthes di Sumatera setiap tahunnya semakin terancam, baik oleh pembalakan liar, kebakaran hutan maupun konversi lahan hutan. Penelitian ini bertujuan untuk menganalisis jenis vegetasi dalam komunitas Nepenthes mirabilis di hutan kampus Institut Agama Islam Negeri Sulthan Thaha Saifuddin Jambi. Penelitian ini merupakan penelitian kualitatif. Metode yang digunakan adalah metode transek. Berdasarkan hasil pengamatan tumbuhan yang ditemukan di sekitar komunitas Nepenthes mirabilis pada plot pengamatan adalah sebanyak 17 famili, yang terdiri dari 26 jenis dan 3547 individu. Jenis tumbuhan yang paling banyak ditemukan di sekitar komunitas Nepenthes mirabilis adalah dari famili Poaceae yaitu Axonopus compressus sebanyak 1336 individu, pada famili Gleicheniaceae yaitu Dicranopteris linearis sebanyak 470 individu dan pada famili Leguminoceae yaitu Mimosa pudica sebanyak 419 individu. Sedangkan jenis tumbuhan yang paling sedikit ditemukan pada famili Polygalaceae dengan jenis Polygala paniculata terdapat 6 individu.   Kata Kunci: Vegetasi, Komunitas, Nepenthes mirabilis.

  3. Increased incidence of urolithiasis and bacteremia during Proteus mirabilis and Providencia stuartii coinfection due to synergistic induction of urease activity.

    Science.gov (United States)

    Armbruster, Chelsie E; Smith, Sara N; Yep, Alejandra; Mobley, Harry L T

    2014-05-15

    Catheter-associated urinary tract infections (CaUTIs) are the most common hospital-acquired infections worldwide and are frequently polymicrobial. The urease-positive species Proteus mirabilis and Providencia stuartii are two of the leading causes of CaUTIs and commonly co-colonize catheters. These species can also cause urolithiasis and bacteremia. However, the impact of coinfection on these complications has never been addressed experimentally. A mouse model of ascending UTI was utilized to determine the impact of coinfection on colonization, urolithiasis, and bacteremia. Mice were infected with P. mirabilis or a urease mutant, P. stuartii, or a combination of these organisms. In vitro experiments were conducted to assess growth dynamics and impact of co-culture on urease activity. Coinfection resulted in a bacterial load similar to monospecies infection but with increased incidence of urolithiasis and bacteremia. These complications were urease-dependent as they were not observed during coinfection with a P. mirabilis urease mutant. Furthermore, total urease activity was increased during co-culture. We conclude that P. mirabilis and P. stuartii coinfection promotes urolithiasis and bacteremia in a urease-dependent manner, at least in part through synergistic induction of urease activity. These data provide a possible explanation for the high incidence of bacteremia resulting from polymicrobial CaUTI.

  4. Proteus mirabilis uroepithelial cell adhesin (UCA) fimbria plays a role in the colonization of the urinary tract.

    Science.gov (United States)

    Pellegrino, Rafael; Scavone, Paola; Umpiérrez, Ana; Maskell, Duncan J; Zunino, Pablo

    2013-03-01

    Urinary tract infections (UTIs) are among the most common bacterial infections in humans. Proteus mirabilis is an opportunistic pathogen, capable of causing severe UTIs, with serious kidney damage that may even lead to death. Several virulence factors are involved in the pathogenicity of this bacterium. Among these, adherence to the uroepithelium mediated by fimbriae appears to be a significant bacterial attribute related to urovirulence. Proteus mirabilis expresses several types of fimbriae that could be involved in the pathogenesis of UTI, including uroepithelial cell adhesin (UCA). In this report, we used an uropathogenic P. mirabilis wild-type strain and an isogenic ucaA mutant unable to express UCA to study the pathogenic role of this fimbria in UTI. Ability of the mutant to adhere to desquamated uroepithelial cells and to infect mice using different experimental UTI models was significantly impaired. These results allow us to conclude that P. mirabilis UCA plays an important role in the colonization of the urinary tract. © 2013 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.

  5. Modulation of pulmonary defense mechanisms by acute exposures to nitrogen dioxide. [Staphylococcus aureus; Proteus mirabilis; Pasteurella pneumotropica

    Energy Technology Data Exchange (ETDEWEB)

    Jakab, G.J.

    1987-02-01

    The effect of acute exposures to NO/sub 2/ on the antibacterial defenses of the murine lung was assessed following inhalation challenges with Staphylococcus aureus, Proteus mirabilis, and Pasteurella pneumotropica. With S. aureus pulmonary antibacterial defenses were suppressed at NO/sub 2/ levels of 4.0 ppm and greater. Exposure to 10.0 ppm enhanced the intrapulmonary killing of P. mirabilis which correlated with an increase in the phagocytic cell populations lavaged from the lungs; at 20.0 ppm bactericidal activity against P. mirabilis was impaired. Pulmonary antibacterial defenses against P. pneumotropica were impaired at 10.0 ppm which correlated with a decrease in the retrieved phagocytic lung cell population. Reversing the order of treatment (ie., NO/sub 2/ exposure prior to bacterial challenge) raised the threshold concentration for NO/sub 2/-induced impairment of intrapulmonary bacterial killing. With S. aureus the effect was not observed at 5.0 ppm but at 10.0 ppm and with P. mirabilis not at 20.0 ppm but at 30.0 ppm intrapulmonary killing was enhanced. Exposures up to 20.0 ppm of NO/sub 2/ did not effect the physical translocation mechanisms of the lung as quantitated by declines in pulmonary radiotracer activity following aerogenic challenge with /sup 32/P-labeled staphylococci.

  6. Inhibition of crystallization caused by Proteus mirabilis during the development of infectious urolithiasis by various phenolic substances.

    Science.gov (United States)

    Torzewska, Agnieszka; Rozalski, Antoni

    2014-01-01

    Infectious urolithiasis is a consequence of persistent urinary tract infections caused by urease producing bacteria e.g. Proteus mirabilis. These stones are composed of struvite and carbonate apatite. Their rapid growth and high recurrence indicate that so far appropriate methods of treatment have not been found. In the present study, the inhibitory effect of phenolic compounds was investigated in vitro against formation of struvite/apatite crystals. The impact of these substances with different chemical structures on crystallization caused by clinical isolates of P. mirabilis was tested spectrophotometrically using a microdilution method. Among the 11 tested compounds resveratrol, epigallocatechin gallate, peralgonidin, vanillic and coffee acids at the concentrations 250-1000 μg/ml inhibited P. mirabilis urease activity and crystallization. However, only vanillic acid had such an effect on all tested strains of P. mirabilis. Therefore, using an in vitro model, bacterial growth, crystallization, urease activity and pH were examined for 24h in synthetic urine with vanillic acid. Effect of vanillic acid was compared with that of other known struvite/apatite crystallization inhibitors (acetohydroxamic acid, pyrophosphate) and it was shown that vanillic acid strongly inhibited bacterial growth and the formation of crystals. It can be assumed that this compound, after further studies, can be used in the treatment or prophylaxis of infectious urolithiasis. Copyright © 2013 Elsevier GmbH. All rights reserved.

  7. Post-irradiation replication and repair in UV-irradiated cells of Proteus mirabilis depends on protein synthesis and a functioning rec+ gene

    International Nuclear Information System (INIS)

    Hofemeister, J.

    1977-01-01

    The amount of and the molecular weight of newly synthesized DNA (piDNA) as well as its repair after UV irradiation in excision-proficient strains of P.mirabilis and E.coli K12 have been compared. A fraction of post-replication repair (PRR) in P.mirabilis is found to be dependent on de novo protein synthesis after UV irradiation. Pre-irradiation by UV and pre-treatment with nalidixic acid increase the efficiency of post-irradiation replication and PRR even in the presence of chloramphenicol. An inducible repair function in P.mirabilis is supposed to stimulate post-irradiation replication and repair. (author)

  8. Post-irradiation replication and repair in uv-irradiated cells of Proteus mirabilis depends on protein synthesis and a functioning rec/sup +/ gene

    Energy Technology Data Exchange (ETDEWEB)

    Hofemeister, J [Akademie der Wissenschaften der DDR, Gatersleben. Zentralinstitut fuer Genetik und Kulturpflanzenforschung

    1977-02-28

    The amount of and the molecular weight of newly synthesized DNA (piDNA) as well as its repair after uv irradiation in excision-proficient strains of P.mirabilis and E.coli K12 have been compared. A fraction of post-replication repair (PRR) in P.mirabilis is found to be dependent on de novo protein synthesis after uv irradiation. Pre-irradiation by uv and pre-treatment with nalidixic acid increase the efficiency of post-irradiation replication and PRR even in the presence of chloramphenicol. An inducible repair function in P.mirabilis is supposed to stimulate post-irradiation replication and repair.

  9. Mini-Mu insertions in the tetracycline resistance determinant from Proteus mirabilis

    Directory of Open Access Journals (Sweden)

    Magalhães V.D.

    1997-01-01

    Full Text Available The inducible tetracycline resistance determinant isolated from Proteus mirabilis cloned into the plasmid pACYC177 was mutagenized by insertion of a mini-Mu-lac phage in order to define the regions in the cloned sequences encoding the structural and regulatory proteins. Three different types of mutants were obtained: one lost the resistance phenotype and became Lac+; another expressed the resistance at lower levels and constitutively; the third was still dependent on induction but showed a lower minimal inhibitory concentration. The mutant phenotypes and the locations of the insertions indicate that the determinant is composed of a repressor gene and a structural gene which are not transcribed divergently as are other known tetracycline determinants isolated from Gram-negative bacteria

  10. Mathemimetics II. Demonstratio Mirabilis of FLT by infinitely ascending cubical crystal growth

    Science.gov (United States)

    Trell, Erik

    2012-09-01

    Emulating Nature by observation and ground-up application of its patterns, structures and processes is a classical scientific practice which under the designation of Biomimetics has now been brought to the Nanotechnology scale where even highly complex systems can be realized by continuous or cyclically reiterated assembly of the respective self-similar eigen-elements, modules and algorithms right from their infinitesimal origin. This is actually quite akin to the genuine mathematical art and can find valuable renewed use as here exemplified by the tentatively original Demonstratio Mirabilis of FLT (Fermat's Last Theorem, or, in that case, Triumph) by infinitely ascending sheet-wise cubical crystal growth leading to the binomial `magic triangle' of his close fellow Blaise Pascal.

  11. Andean cutaneous leishmaniasis (Andean-CL, uta) in Peru and Ecuador: the vector Lutzomyia sand flies and reservoir mammals.

    Science.gov (United States)

    Hashiguchi, Yoshihisa; Gomez L, Eduardo A; Cáceres, Abraham G; Velez, Lenin N; Villegas, Nancy V; Hashiguchi, Kazue; Mimori, Tatsuyuki; Uezato, Hiroshi; Kato, Hirotomo

    2018-02-01

    The vector Lutzomyia sand flies and reservoir host mammals of the Leishmania parasites, causing the Andean cutaneous leishmaniasis (Andean-CL, uta) in Peru and Ecuador were thoroughly reviewed, performing a survey of literatures including our unpublished data. The Peruvian L. (V.) peruviana, a principal Leishmania species causing Andean-CL in Peru, possessed three Lutzomyia species, Lu. peruensis, Lu. verrucarum and Lu. ayacuchensis as vectors, while the Ecuadorian L. (L.) mexicana parasite possessed only one species Lu. ayacuchensis as the vector. Among these, the Ecuadorian showed a markedly higher rate of natural Leishmania infections. However, the monthly and diurnal biting activities were mostly similar among these vector species was in both countries, and the higher rates of infection (transmission) reported, corresponded to sand fly's higher monthly-activity season (rainy season). The Lu. tejadai sand fly participated as a vector of a hybrid parasite of L. (V.) braziliensis/L. (V.) peruviana in the Peruvian Andes. Dogs were considered to be principal reservoir hosts of the L. (V.) peruviana and L. (L.) mexicana parasites in both countries, followed by other sylvatic mammals such as Phyllotis andium, Didelphis albiventris and Akodon sp. in Peru, and Rattus rattus in Ecuador, but information on the reservoir hosts/mammals was extremely poor in both countries. Thus, the Peruvian disease form demonstrated more complicated transmission dynamics than the Ecuadorian. A brief review was also given to the control of vector and reservoirs in the Andes areas. Such information is crucial for future development of the control strategies of the disease. Copyright © 2017 Elsevier B.V. All rights reserved.

  12. Activity of Proteus mirabilis FliL is viscosity dependent and requires extragenic DNA.

    Science.gov (United States)

    Lee, Yi-Ying; Patellis, Julius; Belas, Robert

    2013-02-01

    Proteus mirabilis is a urinary tract pathogen and well known for its ability to move over agar surfaces by flagellum-dependent swarming motility. When P. mirabilis encounters a highly viscous environment, e.g., an agar surface, it differentiates from short rods with few flagella to elongated, highly flagellated cells that lack septa and contain multiple nucleoids. The bacteria detect a surface by monitoring the rotation of their flagellar motors. This process involves an enigmatic flagellar protein called FliL, the first gene in an operon (fliLMNOPQR) that encodes proteins of the flagellar rotor switch complex and flagellar export apparatus. We used a fliL knockout mutant to gain further insight into the function of FliL. Loss of FliL results in cells that cannot swarm (Swr(-)) but do swim (Swm(+)) and produces cells that look like wild-type swarmer cells, termed "pseudoswarmer cells," that are elongated, contain multiple nucleoids, and lack septa. Unlike swarmer cells, pseudoswarmer cells are not hyperflagellated due to reduced expression of flaA (the gene encoding flagellin), despite an increased transcription of both flhD and fliA, two positive regulators of flagellar gene expression. We found that defects in fliL prevent viscosity-dependent sensing of a surface and viscosity-dependent induction of flaA transcription. Studies with fliL cells unexpectedly revealed that the fliL promoter, fliL coding region, and a portion of fliM DNA are needed to complement the Swr(-) phenotype. The data support a dual role for FliL as a critical link in sensing a surface and in the maintenance of flagellar rod integrity.

  13. Climate change forces new ecological states in tropical Andean lakes.

    Directory of Open Access Journals (Sweden)

    Neal Michelutti

    Full Text Available Air temperatures in the tropical Andes have risen at an accelerated rate relative to the global average over recent decades. However, the effects of climate change on Andean lakes, which are vital to sustaining regional biodiversity and serve as an important water resource to local populations, remain largely unknown. Here, we show that recent climate changes have forced alpine lakes of the equatorial Andes towards new ecological and physical states, in close synchrony to the rapid shrinkage of glaciers regionally. Using dated sediment cores from three lakes in the southern Sierra of Ecuador, we record abrupt increases in the planktonic thalassiosiroid diatom Discostella stelligera from trace abundances to dominance within the phytoplankton. This unprecedented shift occurs against the backdrop of rising temperatures, changing atmospheric pressure fields, and declining wind speeds. Ecological restructuring in these lakes is linked to warming and/or enhanced water column stratification. In contrast to seasonally ice-covered Arctic and temperate alpine counterparts, aquatic production has not increased universally with warming, and has even declined in some lakes, possibly because enhanced thermal stability impedes the re-circulation of hypolimnetic nutrients to surface waters. Our results demonstrate that these lakes have already passed important ecological thresholds, with potentially far-reaching consequences for Andean water resources.

  14. Genome-wide transposon mutagenesis of Proteus mirabilis: Essential genes, fitness factors for catheter-associated urinary tract infection, and the impact of polymicrobial infection on fitness requirements

    Science.gov (United States)

    Smith, Sara N.; Zhao, Lili; Wu, Weisheng

    2017-01-01

    The Gram-negative bacterium Proteus mirabilis is a leading cause of catheter-associated urinary tract infections (CAUTIs), which are often polymicrobial. Numerous prior studies have uncovered virulence factors for P. mirabilis pathogenicity in a murine model of ascending UTI, but little is known concerning pathogenesis during CAUTI or polymicrobial infection. In this study, we utilized five pools of 10,000 transposon mutants each and transposon insertion-site sequencing (Tn-Seq) to identify the full arsenal of P. mirabilis HI4320 fitness factors for single-species versus polymicrobial CAUTI with Providencia stuartii BE2467. 436 genes in the input pools lacked transposon insertions and were therefore concluded to be essential for P. mirabilis growth in rich medium. 629 genes were identified as P. mirabilis fitness factors during single-species CAUTI. Tn-Seq from coinfection with P. stuartii revealed 217/629 (35%) of the same genes as identified by single-species Tn-Seq, and 1353 additional factors that specifically contribute to colonization during coinfection. Mutants were constructed in eight genes of interest to validate the initial screen: 7/8 (88%) mutants exhibited the expected phenotypes for single-species CAUTI, and 3/3 (100%) validated the expected phenotypes for polymicrobial CAUTI. This approach provided validation of numerous previously described P. mirabilis fitness determinants from an ascending model of UTI, the discovery of novel fitness determinants specifically for CAUTI, and a stringent assessment of how polymicrobial infection influences fitness requirements. For instance, we describe a requirement for branched-chain amino acid biosynthesis by P. mirabilis during coinfection due to high-affinity import of leucine by P. stuartii. Further investigation of genes and pathways that provide a competitive advantage during both single-species and polymicrobial CAUTI will likely provide robust targets for therapeutic intervention to reduce P. mirabilis

  15. Survey of multidrug resistance integrative mobilizable elements SGI1 and PGI1 in Proteus mirabilis in humans and dogs in France, 2010-13.

    Science.gov (United States)

    Schultz, Eliette; Haenni, Marisa; Mereghetti, Laurent; Siebor, Eliane; Neuwirth, Catherine; Madec, Jean-Yves; Cloeckaert, Axel; Doublet, Benoît

    2015-09-01

    To characterize MDR genomic islands related to Salmonella genomic island 1 (SGI1) and Proteus genomic island 1 (PGI1) in Proteus mirabilis from human and animal sources in France in light of the previously reported cases. A total of 52 and 46 P. mirabilis clinical strains from human and animal sources, respectively, were studied for the period 2010-13. MDR was assessed by antimicrobial susceptibility testing, PCR detection of SGI1 and PGI1 and PCR mapping of the MDR regions. The diversity of the SGI1/PGI1-positive P. mirabilis strains was assessed by PFGE. Twelve P. mirabilis strains (5 humans and 7 dogs) were found to harbour an MDR island related to SGI1 or PGI1. Among them, several SGI1 variants were identified in diverse P. mirabilis genetic backgrounds. The variant SGI1-V, which harbours the ESBL bla VEB-6 gene, was found in closely genetically related human and dog P. mirabilis strains. The recently described PGI1 element was also identified in human and dog strains. Finally, one strain harboured a novel SGI genomic island closely related to SGI1 and SGI2 without an insertion of the MDR region. This study reports for the first time, to our knowledge, SGI1-positive and PGI1-positive P. mirabilis strains from dogs in France. The genetic diversity of the strains suggests several independent horizontal acquisitions of these MDR elements. The potential transmission of SGI1/PGI1-positive P. mirabilis strains between animals and humans is of public health concern, notably with regard to the spread of ESBL and carbapenemase genes, i.e. bla VEB-6 and bla NDM-1. © The Author 2015. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

  16. The impact of production of extended-spectrum β-lactamases on the 28-day mortality rate of patients with Proteus mirabilis bacteremia in Korea.

    Science.gov (United States)

    Ahn, Jin Young; Ann, Hea Won; Jeon, Yongduk; Ahn, Mi Young; Oh, Dong Hyun; Kim, Yong Chan; Kim, Eun Jin; Song, Je Eun; Jung, In Young; Kim, Moo Hyun; Jeong, Wooyoung; Ku, Nam Su; Jeong, Su Jin; Choi, Jun Yong; Yong, Dongeun; Song, Young Goo; Kim, June Myung

    2017-05-03

    The incidence of Proteus mirabilis antimicrobial resistance, especially that mediated by extended-spectrum β-lactamases (ESBLs), has increased. We investigated the impact of ESBL production on the mortality of patients with P. mirabilis bacteremia in Korea. Patients diagnosed with P. mirabilis bacteremia between November 2005 and December 2013 at a 2000-bed tertiary care center in South Korea were included in this study. Phenotypic and molecular analyses were performed to assess ESBL expression. Characteristics and treatment outcomes were investigated among ESBL-producing and non-ESBL-producing P. mirabilis bacteremia groups. A multivariate analysis of 28-day mortality rates was performed to evaluate the independent impact of ESBLs. Among 62 P. mirabilis isolates from 62 patients, 14 expressed ESBLs (CTX-M, 2; TEM, 5; both, 6; other, 1), and the 28-day mortality rate of the 62 patients was 17.74%. No clinical factor was significantly associated with ESBL production. The 28-day mortality rate in the ESBL-producing group was significantly higher than that in the non-ESBL-producing group (50% vs. 8.3%, p = 0.001). A multivariate analysis showed that ESBL production (odds ratio [OR], 11.53, 95% confidence interval [CI], 2.11-63.05, p = 0.005) was independently associated with the 28-day mortality rate in patients with P. mirabilis bacteremia. ESBL production is significantly associated with mortality in patients with bacteremia caused by P. mirabilis. Rapid detection of ESBL expression and prompt appropriate antimicrobial therapy are required to reduce mortality caused by P. mirabilis bacteremia.

  17. Genome-wide transposon mutagenesis of Proteus mirabilis: Essential genes, fitness factors for catheter-associated urinary tract infection, and the impact of polymicrobial infection on fitness requirements.

    Science.gov (United States)

    Armbruster, Chelsie E; Forsyth-DeOrnellas, Valerie; Johnson, Alexandra O; Smith, Sara N; Zhao, Lili; Wu, Weisheng; Mobley, Harry L T

    2017-06-01

    The Gram-negative bacterium Proteus mirabilis is a leading cause of catheter-associated urinary tract infections (CAUTIs), which are often polymicrobial. Numerous prior studies have uncovered virulence factors for P. mirabilis pathogenicity in a murine model of ascending UTI, but little is known concerning pathogenesis during CAUTI or polymicrobial infection. In this study, we utilized five pools of 10,000 transposon mutants each and transposon insertion-site sequencing (Tn-Seq) to identify the full arsenal of P. mirabilis HI4320 fitness factors for single-species versus polymicrobial CAUTI with Providencia stuartii BE2467. 436 genes in the input pools lacked transposon insertions and were therefore concluded to be essential for P. mirabilis growth in rich medium. 629 genes were identified as P. mirabilis fitness factors during single-species CAUTI. Tn-Seq from coinfection with P. stuartii revealed 217/629 (35%) of the same genes as identified by single-species Tn-Seq, and 1353 additional factors that specifically contribute to colonization during coinfection. Mutants were constructed in eight genes of interest to validate the initial screen: 7/8 (88%) mutants exhibited the expected phenotypes for single-species CAUTI, and 3/3 (100%) validated the expected phenotypes for polymicrobial CAUTI. This approach provided validation of numerous previously described P. mirabilis fitness determinants from an ascending model of UTI, the discovery of novel fitness determinants specifically for CAUTI, and a stringent assessment of how polymicrobial infection influences fitness requirements. For instance, we describe a requirement for branched-chain amino acid biosynthesis by P. mirabilis during coinfection due to high-affinity import of leucine by P. stuartii. Further investigation of genes and pathways that provide a competitive advantage during both single-species and polymicrobial CAUTI will likely provide robust targets for therapeutic intervention to reduce P. mirabilis

  18. Cefepime shows good efficacy and no antibiotic resistance in pneumonia caused by Serratia marcescens and Proteus mirabilis - an observational study.

    Science.gov (United States)

    Yayan, Josef; Ghebremedhin, Beniam; Rasche, Kurt

    2016-03-23

    Many antibiotics have no effect on Gram-positive and Gram-negative microbes, which necessitates the prescription of broad-spectrum antimicrobial agents that can lead to increased risk of antibiotic resistance. These pathogens constitute a further threat because they are also resistant to numerous beta-lactam antibiotics, as well as other antibiotic groups. This study retrospectively investigates antimicrobial resistance in hospitalized patients suffering from pneumonia triggered by Gram-negative Serratia marcescens or Proteus mirabilis. The demographic and clinical data analyzed in this study were obtained from the clinical databank of the HELIOS Clinic, Witten/Herdecke University, Wuppertal, Germany, for inpatients presenting with pneumonia triggered by S. marcescens or P. mirabilis from 2004 to 2014. An antibiogram was conducted for the antibiotics utilized as part of the management of patients with pneumonia triggered by these two pathogens. Pneumonia was caused by Gram-negative bacteria in 115 patients during the study period from January 1, 2004, to August 12, 2014. Of these, 43 (37.4 %) hospitalized patients [26 males (60.5 %, 95 % CI 45.9 %-75.1 %) and 17 females (39.5 %, 95 % CI 24.9 %-54.1 %)] with mean age of 66.2 ± 13.4 years had pneumonia triggered by S. marcescens, while 20 (17.4 %) patients [14 males (70 %, 95 % CI 49.9 %-90.1 %) and 6 females (30 %, 95 % CI 9.9 %-50.1 %)] with a mean age of 64.6 ± 12.8 years had pneumonia caused by P. mirabilis. S. marcescens showed an increased antibiotic resistance to ampicillin (100 %), ampicillin-sulbactam (100 %), and cefuroxime (100 %). P. mirabilis had a high resistance to tetracycline (100 %) and ampicillin (55 %). S. marcescens (P < 0.0001) and P. mirabilis (P = 0.0003) demonstrated no resistance to cefepime in these patients with pneumonia. S. marcescens and P. mirabilis were resistant to several commonly used antimicrobial agents, but showed no resistance to

  19. Neogene stratigraphy and Andean geodynamics of southern Ecuador

    Science.gov (United States)

    Hungerbühler, Dominik; Steinmann, Michael; Winkler, Wilfried; Seward, Diane; Egüez, Arturo; Peterson, Dawn E.; Helg, Urs; Hammer, Cliff

    2002-01-01

    The present paper reviews Tertiary volcanic and sedimentary formations in the Inter-Andean region of southern Ecuador (between 2°S and 4°20'S) in order to develop a geodynamic model of the region. The formations occur in the southern shallow prolongation of the Inter-Andean Valley between the Cordillera Real to the east, and the Cordillera Occidental and Amotape-Tahuín Provinces to the west. One hundred fifty zircon fission-track analyses has established a detailed chronostratigraphy for the sedimentary and volcanic formations and several small intrusions. The Paleogene to early Miocene formations are dominated by intermediate and acidic volcanic and pyroclastic rocks. In addition, relics of Eocene continental sedimentary series have been identified. The Neogene sedimentary series lie unconformably on deformed and eroded metamorphic, sedimentary and volcanic formations. They were deposited in two stages, which are separated by a major unconformity dated at ≈10-9 Ma. (1) During the middle and early late Miocene (≈15-10 Ma) marginal marine deltaic, lagoonal, lacustrine and fluvial environments prevailed, which we group under the heading "Pacific Coastal sequences". They presumably covered a greater surface area in southern Ecuador than their present occurrence in small topographic depressions. We suggest that they were deposited in the shallow marine Cuenca and Loja Embayments. Deposition in a marginal marine environment is also supported by the occurrence of brackish water ostracods and other fauna. (2) Above the regional (angular) unconformity, the coastal facies are overlain by late Miocene (≈9-5 Ma) continental alluvial fan and fluvial facies which are in turn covered by mainly airborne volcanic material. They represent the "Intermontane sequences" of the basins of Cuenca, Girón-Santa Isabel, Nabón, Loja and Malacatos-Vilcabamba. Sedimentologic and stratigraphic results are used to discuss the tectonic setting of Neogene sedimentation in the forearc

  20. The evolution of witchcraft and the meaning of healing in colonial Andean society.

    Science.gov (United States)

    Silverblatt, I

    1983-12-01

    This paper explores the ways in which traditional beliefs of Andean peoples regarding health and sickness were transformed by the process of Spanish colonization. It also examines how the colonial context devolved new meanings and powers on native curers. The analysis of these transformations in Andean systems of meanings and role structures relating to healing depends on an examination of the European witchcraze of the 16th-17th centuries. The Spanish conquest of the Inca empire in the mid-1500's coincided with the European witch hunts; it is argued that the latter formed the cultural lens through which the Spanish evaluated native religion--the matrix through which Andean concepts of disease and health were expressed--as well as native curers. Andean religion was condemned as heresy and curers were condemned as witches. Traditional Andean cosmology was antithetical to 16th century European beliefs in the struggle between god and the devil, between loyal Christians and the Satan's followers. Consequently, European concepts of disease and health based on the power of witches, Satan's adherents, to cause harm and cure were alien to pre-Columbian Andean thought. Ironically European concepts of Satan and the supposed powers of witches began to graft themselves onto the world view of Andean peoples. The ensuing dialectic of ideas as well as the creation of new healers/witches forged during the imposition of colonial rule form the crux of this analysis.

  1. Occurrence of Proteus mirabilis associated with two species of venezuelan oysters Ocorrência de Proteus mirabilis associado a duas espécies de ostras venezuelanas

    Directory of Open Access Journals (Sweden)

    Milagro Fernández-Delgado

    2007-12-01

    Full Text Available The fecal contamination of raw seafood by indicators and opportunistic pathogenic microorganisms represents a public health concern. The objective of this study was to investigate the presence of enteric bacteria colonizing oysters collected from a Venezuelan touristic area. Oyster samples were collected at the northwestern coast of Venezuela and local salinity, pH, temperature, and dissolved oxygen of seawater were recorded. Total and fecal coliforms were measured for the assessment of the microbiological quality of water and oysters, using the Multiple Tube Fermentation technique. Analyses were made using cultures and 16S rRNA gene sequencing. Diverse enrichment and selective culture methods were used to isolate enteric bacteria. We obtained pure cultures of Gram-negative straight rods with fimbriae from Isognomon alatus and Crassostrea rhizophorae. Our results show that P. mirabilis was predominant under our culture conditions. We confirmed the identity of the cultures by biochemical tests, 16S rRNA gene sequencing, and data analysis. Other enterobacteria such as Escherichia coli, Morganella morganii and Klebsiella pneumoniae were also isolated from seawater and oysters. The presence of pathogenic bacteria in oysters could have serious epidemiological implications and a potential human health risk associated with consumption of raw seafood.A contaminação fecal de frutos do mar crus por microrganismos oportunistas patogênicos representa problema de saúde pública. O objetivo deste estudo é investigar a presença de bactérias entéricas que colonizam ostras coletadas em área turística da Venezuela. Amostras de ostras foram coletadas na costa noroeste da Venezuela e foram registrados a salinidade local, pH, temperatura e o oxigênio dissolvido na água do mar. O total de coliformes fecais foi medido para a avaliação da qualidade microbiológica da água e das ostras, usando a técnica de fermentação em tubos múltiplos. An

  2. Epidemiology of extended-spectrum β-lactamase, AmpC, and carbapenemase production in Proteus mirabilis.

    Science.gov (United States)

    Datta, Priya; Gupta, Varsha; Arora, Shilpa; Garg, Shivani; Chander, Jagdish

    2014-01-01

    Proteus mirabilis strains that produce extended-spectrum β-lactamase (ESBL), AmpC β-lactamase, and carbapenemase pose potential threats to patient care because most clinical diagnostic laboratories may not attempt to detect these three major groups of enzymes. Therefore, the objective of this study was to ascertain if P. mirabilis isolates collected from our heathcare facility possess various mechanisms of resistance to β-lactams (i.e., ESBL, AmpC, and carbapenemases) and to additionally arrive at conclusions regarding concurrent testing for these three mechanism of drug resistance in order to reduce cost and time in routine diagnostic testing. Between January 2011 and June 2011, 60 consecutive non-repeated strains of P. mirabilis were evaluated for production of ESBLs, AmpC β-lactamases, and carbapenemases. Of these, 36 isolates were found to be ESBL producers, and 7 (12%) were positive for production of AmpC β-lactamases and ESBLs. Therefore, 19.4% of ESBL-producing Proteus strains coproduced AmpC enzymes. The modified Hodge test confirmed carbapenemase production in only 1 isolate (1.7%), which was also ESBL- and AmpC-positive. The clinical impact of additional AmpC expression in ESBL-producing P. mirabilis results in a newly acquired resistance to β-lactamase inhibitors. In addition, to save time and costs, we recommend the use of cefepime/cefepime-clavulanate or boronic acid for the ESBL detection but in only those strains that were positive for ESBL by screening and negative by confirmatory tests.

  3. Evaluation of environmental scanning electron microscopy for analysis of Proteus mirabilis crystalline biofilms in situ on urinary catheters.

    Science.gov (United States)

    Holling, Nina; Dedi, Cinzia; Jones, Caroline E; Hawthorne, Joseph A; Hanlon, Geoffrey W; Salvage, Jonathan P; Patel, Bhavik A; Barnes, Lara M; Jones, Brian V

    2014-06-01

    Proteus mirabilis is a common cause of catheter-associated urinary tract infections and frequently leads to blockage of catheters due to crystalline biofilm formation. Scanning electron microscopy (SEM) has proven to be a valuable tool in the study of these unusual biofilms, but entails laborious sample preparation that can introduce artefacts, undermining the investigation of biofilm development. In contrast, environmental scanning electron microscopy (ESEM) permits imaging of unprocessed, fully hydrated samples, which may provide much insight into the development of P. mirabilis biofilms. Here, we evaluate the utility of ESEM for the study of P. mirabilis crystalline biofilms in situ, on urinary catheters. In doing so, we compare this to commonly used conventional SEM approaches for sample preparation and imaging. Overall, ESEM provided excellent resolution of biofilms formed on urinary catheters and revealed structures not observed in standard SEM imaging or previously described in other studies of these biofilms. In addition, we show that energy-dispersive X-ray spectroscopy (EDS) may be employed in conjunction with ESEM to provide information regarding the elemental composition of crystalline structures and demonstrate the potential for ESEM in combination with EDS to constitute a useful tool in exploring the mechanisms underpinning crystalline biofilm formation. © 2014 The Authors. FEMS Microbiology Letters published by John Wiley & Sons Ltd on behalf of Federation of European Microbiological Societies.

  4. Regional Spread of CTX-M-2-Producing Proteus mirabilis with the Identical Genetic Structure in Japan.

    Science.gov (United States)

    Kato, Karin; Matsumura, Yasufumi; Yamamoto, Masaki; Nagao, Miki; Takakura, Shunji; Ichiyama, Satoshi

    2017-07-01

    In this study, we analyzed the molecular epidemiology of extended-spectrum β-lactamase (ESBL)-producing Proteus mirabilis isolates collected from the central region of Japan. Between 2005 and 2012, 820 clinical P. mirabilis isolates were obtained from ten acute care hospitals in Japan. We characterized ESBL confirmatory test-positive isolates by sequencing the ESBL genes and their flanking regions, detecting plasmid replicons, and performing pulsed-field gel electrophoresis (PFGE). Ninety-six isolates (12%) were positive according to the ESBL confirmatory test; all these isolates possessed bla CTX-M-2 with the same flanking structure of upstream ΔISEcp1 and a downstream region identical to downstream bla KLUA-1 . IncT was the prevalent, and only, replicon found in 63 isolates. PFGE analysis detected eight clusters with more than one isolate, among which three included 56 isolates and six included isolates from multiple hospitals. CTX-M-2-producing P. mirabilis with an identical genetic structure flanking bla CTX-M-2 is dominant in this Japanese region, and there is evidence for the clonal spread of isolates.

  5. Distribution and antimicrobial susceptibility profile of extended-spectrum β-lactamase-producing Proteus mirabilis strains recently isolated in Japan.

    Science.gov (United States)

    Kanayama, Akiko; Kobayashi, Intetsu; Shibuya, Kazutoshi

    2015-02-01

    Here we report on the prevalence of extended-spectrum β-lactamase (ESBL)-producing Proteus mirabilis from a nationwide antimicrobial resistance survey in different geographical regions of Japan. A total of 799 P. mirabilis isolates recovered between July 2009 and June 2010 from 314 healthcare facilities were characterised according to ESBL production, source, location and antimicrobial susceptibility pattern. ESBL production was found in 364 (45.6%) of the isolates, among which 354 (97.3%) produced CTX-M-2 group β-lactamases. Of the 349 ESBL-producing isolates in which the inpatient or outpatient status of the source was known, 324 (92.8%) were from inpatients and 25 (7.2%) were from outpatients (Pmirabilis compared with 159/405 (39.3%), 119/209 (56.9%), 42/77 (54.5%) and 20/49 (40.8%), respectively, for isolates from urine, sputum, decubitus ulcer and wound specimens. Among the ESBL-producers, non-susceptibility to ciprofloxacin was found in 74.2% of the ESBL-producing isolates compared with 17.7% of the ESBL-non-producing isolates. These results show that approximately one-half of the P. mirabilis isolates from clinical specimens in Japan are ESBL-producers and that the potential for concomitant fluoroquinolone resistance must also be considered. Copyright © 2014 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved.

  6. A new minute Andean Pristimantis (Anura: Strabomantidae from Venezuela

    Directory of Open Access Journals (Sweden)

    César L. Barrio-Amorós

    2012-12-01

    Full Text Available A new species of Pristimantis is described from the Venezuelan Andes. The new species is the smallest in its genus known in Venezuela and belongs to the Pristimantis unistrigatus Group. It differs from the rest of Venezuelan Andean congeners in body size (mean male SVL < 21.3 mm, female SVL < 26.3 mm, expanded discs on fingers and toes, absence of dorsolateral folds, and a distinctivecall consisting in 2–5 cricket-like short notes. The new species inhabits the southwestern part of the Cordillera de Mérida in Venezuela and the Venezuelan side of the Cordillera Oriental deColombia, and could be present on the Colombian portion of the cordillera as well.

  7. Chlorella mirabilis as a Potential Species for Biomass Production in Low-Temperature Environment.

    Science.gov (United States)

    Shukla, S P; Kvíderová, J; Tříska, J; Elster, J

    2013-01-01

    Successful adaptation/acclimatization to low temperatures in micro-algae is usually connected with production of specific biotechnologically important compounds. In this study, we evaluated the growth characteristics in a micro-scale mass cultivation of the Antarctic soil green alga Chlorella mirabilis under different nitrogen and carbon sources followed by analyses of fatty acid contents. The micro-scale mass cultivation was performed in stable (in-door) and variable (out-door) conditions during winter and/or early spring in the Czech Republic. In the in-door cultivation, the treatments for nitrogen and carbon sources determination included pure Z medium (control, Z), Z medium + 5% glycerol (ZG), Z medium + 5% glycerol + 50 μM KNO3 (ZGN), Z medium + 5% glycerol + 200 μM NH4Cl (ZGA), Z medium + 5% glycerol + 1 mM Na2CO3 (ZNC), Z medium + 5% glycerol + 1 mM Na2CO3 + 200 μM NH4Cl (ZGCA) and Z medium + 5% glycerol + 1 mM Na2CO3 + 50 μM KNO3 (ZGCN) and were performed at 15°C with an irradiance of 75 μmol m(-2) s(-1). During the out-door experiments, the night-day temperature ranged from -6.6 to 17.5°C (daily average 3.1 ± 5.3°C) and irradiance ranged from 0 to 2,300 μmol m(-2) s(-1) (daily average 1,500 ± 1,090 μmol m(-2) s(-1)). Only the Z, ZG, ZGN, and ZGC treatments were used in the out-door cultivation. In the in-door mass cultivation, all nitrogen and carbon sources additions increased the growth rate with the exception of ZGA. When individual sources were considered, only the effect of 5% glycerol addition was significant. On the other hand, the growth rate decreased in the ZG and ZGN treatments in the out-door experiment, probably due to carbon limitation. Fatty acid composition showed increased production of linoleic acid in the glycerol treatments. The studied strain of C. mirabilis is proposed to be a promising source of linoleic acid in low

  8. Chlorella mirabilis as a potential species for biomass production in low temperature environment

    Directory of Open Access Journals (Sweden)

    Satya Prakash Shukla

    2013-04-01

    Full Text Available Successful adaptation/acclimatization to low temperatures in micro-algae is usually connected with production of specific biotechnologically important compounds. In this study, we evaluated the growth characteristics in a micro-scale mass cultivation of the Antarctic soil green alga Chlorella mirabilis under different nitrogen and carbon sources followed by analyses of fatty acid contents. The micro-scale mass cultivation was performed in stable (in-door and variable (out-door conditions during winter and/or early spring in the Czech Republic. In the in-door cultivation, the treatments for nitrogen and carbon sources determination included pure Z medium (control, Z, Z medium + 5% glycerol (ZG, Z medium + 5% glycerol + 50 µM KNO3 (ZGN, Z medium + 5% glycerol + 200µM NH4Cl (ZGA, Z medium + 5% glycerol + 1 mM Na2CO3 (ZNC, Z medium + 5% glycerol + 1 mM Na2CO3 + 200µM NH4Cl (ZGCA and Z medium + 5% glycerol + 1 mM Na2CO3 + 50 µM KNO3 (ZGCN and were performed at 15°C with an irradiance of 75 µmol m-2 s-1. During the out-door experiments, the night-day temperature ranged from -6.6°C to 17.5°C (daily average 3.1±5.3 °C and irradiance ranged from 0 to 2300 µmol m-2 s-1 (daily average 1500±1090 µmol m-2 s-1. Only the Z, ZG, ZGN and ZGC treatments were used in the out-door cultivation. In the in-door mass cultivation, all nitrogen and carbon sources additions increased the growth rate with the exception of ZGA. When individual sources were considered, only the effect of 5% glycerol addition was significant. On the other hand, the growth rate decreased in the ZG and ZGN treatments in the out-door experiment, probably due to carbon limitation. Fatty acid composition showed increased production of linoleic acid in the glycerol treatments. The studied strain of C. mirabilis is proposed to be a promising source of linoleic acid in low temperature mass cultivation biotechnology. This strain is a perspective model organism for biotechnology in low

  9. From Dearth to El Dorado: Andean Nature, Plate Tectonics, and the Ontologies of Ecuadorian Resource Wealth

    Directory of Open Access Journals (Sweden)

    David Kneas

    2018-03-01

    Full Text Available Since the early 1990s, the Ecuadorian government has pledged to convert the nation into a “mining country” of global standing. Contemporary claims of mineral wealth, however, stand in stark contrast to previous assessments. Indeed, through much of the 20th century, geologists described Ecuador as a country of mineral dearth. Exploring the process through which Ecuador seemingly transitioned from a nation of resource scarcity to one of mineral plenty, I demonstrate how assessments of Ecuador’s resource potential relate to ideas of Andean nature. Promoters of resource abundance have emphasized Andean uniformity and equivalence—the notion that Ecuador’s mineral wealth is inevitable by virtue of the resource richness of its Andean neighbors. Geologists who have questioned Ecuador’s mineral content, on the other hand, have emphasized Andean heterogeneity. In the recent promotion of Ecuador’s resource potential, notions of Andean uniformity have been bolstered by models of subsoil copper that emerged in the in 1970s in the context of plate-tectonic theory. In highlighting the linkage between ideas of Andean nature and appraisals of Ecuadorian resource potential since the late 19th century, I outline the dialectics between nature and natural resources that underpin processes of resource becoming.

  10. Bee pollen as non-wood forest product in the eastern Andean highlands of Colombia

    Directory of Open Access Journals (Sweden)

    Fermín José Chamorro García

    2013-07-01

    Full Text Available The Andean forests of the Eastern Andean high-lands of Colombia have a high conservation priority given the vulnerable condition of species such as Quercus humboldtii (Fagaceae that inhabit these ecosystems. Beekeeping is regarded as an alternative activity that could play a role in the conservation of Andean forests, but little is known about how the floras of these ecosystems contribute to honey and bee pollen production. We analyzed the contribution of Andean forests to bee pollen production, given the productive potential and commercial importance of this product. Pollen analyses were performed on 25 samples from apiaries near Andean forests located in the states of Cundinamarca, Boyacá and Santander. We found that Q. humboldtii is an important source of pollen with high potential for monofloral bee pollen production. In addition, bees collect pollen from other Andean forests species such as Weinmannia tomentosa, Viburnum spp. and Morella spp. Utilization of bee pollen could lead to incentives to carry out forest conservation practices through beekeeping management.

  11. Phytoremediation of petroleum contaminated soils by Mirabilis Jalapa L. in a greenhouse plot experiment.

    Science.gov (United States)

    Peng, Shengwei; Zhou, Qixing; Cai, Zhang; Zhang, Zhineng

    2009-09-15

    Phytoremediation of soils contaminated by organic chemicals is a challenging problem in environmental science and engineering. On the basis of identifying remediation plants from ornamentals, the remediation capability of Mirabilis Jalapa L. to treat petroleum contaminated soil from the Shengli Oil Field in Dongying City, Shandong Province, China was further investigated using a field plot experiment carried out in a greenhouse. The results showed that the average efficiency of removing total petroleum hydrocarbons (TPHs) by M. jalapa over the 127-day culture period was high, up to 41.61-63.20%, when the removal rate by natural attenuation was only 19.75-37.92%. The maximum reduction occurred in the saturated hydrocarbon fraction compared with other components of petroleum contaminants. According to the qualitative and quantitative parameters including plant height, fresh weight, dry weight, root length, root weight and visual stress symptoms, it was indicated that M. jalapa had a peculiar tolerance to petroleum contamination and could effectively promote the degradation of TPHs when the concentration of petroleum hydrocarbons in soil was equal to and lower than 10,000 mg/kg. The population of living microorganisms in the planted soil could be also adaptive to contaminated soil. On the whole, M. jalapa is a widely spread species that can be effectively applied to phytoremediation of contaminated soil.

  12. Occurrence of Proteus mirabilis associated with two species of venezuelan oysters.

    Science.gov (United States)

    Fernández-Delgado, Milagro; Contreras, Monica; García-Amado, María Alexandra; Gueneau, Pulchérie; Suárez, Paula

    2007-01-01

    The fecal contamination of raw seafood by indicators and opportunistic pathogenic microorganisms represents a public health concern. The objective of this study was to investigate the presence of enteric bacteria colonizing oysters collected from a Venezuelan touristic area. Oyster samples were collected at the northwestern coast of Venezuela and local salinity, pH, temperature, and dissolved oxygen of seawater were recorded. Total and fecal coliforms were measured for the assessment of the microbiological quality of water and oysters, using the Multiple Tube Fermentation technique. Analyses were made using cultures and 16S rRNA gene sequencing. Diverse enrichment and selective culture methods were used to isolate enteric bacteria. We obtained pure cultures of Gram-negative straight rods with fimbriae from Isognomon alatus and Crassostrea rhizophorae. Our results show that P. mirabilis was predominant under our culture conditions. We confirmed the identity of the cultures by biochemical tests, 16S rRNA gene sequencing, and data analysis. Other enterobacteria such as Escherichia coli, Morganella morganii and Klebsiella pneumoniae were also isolated from seawater and oysters. The presence of pathogenic bacteria in oysters could have serious epidemiological implications and a potential human health risk associated with consumption of raw seafood.

  13. Complicated catheter-associated urinary tract infections due to Escherichia coli and Proteus mirabilis.

    Science.gov (United States)

    Jacobsen, S M; Stickler, D J; Mobley, H L T; Shirtliff, M E

    2008-01-01

    Catheter-associated urinary tract infections (CAUTIs) represent the most common type of nosocomial infection and are a major health concern due to the complications and frequent recurrence. These infections are often caused by Escherichia coli and Proteus mirabilis. Gram-negative bacterial species that cause CAUTIs express a number of virulence factors associated with adhesion, motility, biofilm formation, immunoavoidance, and nutrient acquisition as well as factors that cause damage to the host. These infections can be reduced by limiting catheter usage and ensuring that health care professionals correctly use closed-system Foley catheters. A number of novel approaches such as condom and suprapubic catheters, intermittent catheterization, new surfaces, catheters with antimicrobial agents, and probiotics have thus far met with limited success. While the diagnosis of symptomatic versus asymptomatic CAUTIs may be a contentious issue, it is generally agreed that once a catheterized patient is believed to have a symptomatic urinary tract infection, the catheter is removed if possible due to the high rate of relapse. Research focusing on the pathogenesis of CAUTIs will lead to a better understanding of the disease process and will subsequently lead to the development of new diagnosis, prevention, and treatment options.

  14. A novel biosorbent for dye removal: Extracellular polymeric substance (EPS) of Proteus mirabilis TJ-1

    Energy Technology Data Exchange (ETDEWEB)

    Zhang Zhiqiang [State Key Laboratory of Pollution Control and Resource Reuse, Key Laboratory of Yangtze River Water Environment of Ministry of Education, College of Environmental Science and Engineering, Tongji University, Shanghai 200092 (China); Laboratoire de Sciences Analytiques (UMR CNRS 5180), Universite Claude Bernard Lyon 1, Universite de Lyon, 69622 Villeurbanne Cedex (France); Xia Siqing [State Key Laboratory of Pollution Control and Resource Reuse, Key Laboratory of Yangtze River Water Environment of Ministry of Education, College of Environmental Science and Engineering, Tongji University, Shanghai 200092 (China)], E-mail: siqingxia@mail.tongji.edu.cn; Wang Xuejiang; Yang Aming; Xu Bin; Chen Ling; Zhu Zhiliang; Zhao Jianfu [State Key Laboratory of Pollution Control and Resource Reuse, Key Laboratory of Yangtze River Water Environment of Ministry of Education, College of Environmental Science and Engineering, Tongji University, Shanghai 200092 (China); Jaffrezic-Renault, Nicole; Leonard, Didier [Laboratoire de Sciences Analytiques (UMR CNRS 5180), Universite Claude Bernard Lyon 1, Universite de Lyon, 69622 Villeurbanne Cedex (France)

    2009-04-15

    This paper deals with the extracellular polymeric substance (EPS) of Proteus mirabilis TJ-1 used as a novel biosorbent to remove dye from aqueous solution in batch systems. As a widely used and hazardous dye, basic blue 54 (BB54) was chosen as the model dye to examine the adsorption performance of the EPS. The effects of pH, initial dye concentration, contact time and temperature on the sorption of BB54 to the EPS were examined. At various initial dye concentrations (50-400 mg/L), the batch sorption equilibrium can be obtained in only 5 min. Kinetic studies suggested that the sorption followed the internal transport mechanism. According to the Langmuir model, the maximum BB54 uptake of 2.005 g/g was obtained. Chemical analysis of the EPS indicated the presence of protein (30.9%, w/w) and acid polysaccharide (63.1%, w/w). Scanning electron microscopy (SEM) images showed that the EPS with a crystal-linear structure was whole enwrapped by adsorbed dye molecules. FTIR spectrum result revealed the presence of adsorbing groups such as carboxyl, hydroxyl and amino groups in the EPS. High-molecular weight of the EPS with more binding-sites and stronger van der Waals forces together with its specific construct leads to the excellent performance of dye adsorption. The EPS shows potential board application as a biosorbent for both environmental protection and dye recovery.

  15. A novel biosorbent for dye removal: Extracellular polymeric substance (EPS) of Proteus mirabilis TJ-1

    International Nuclear Information System (INIS)

    Zhang Zhiqiang; Xia Siqing; Wang Xuejiang; Yang Aming; Xu Bin; Chen Ling; Zhu Zhiliang; Zhao Jianfu; Jaffrezic-Renault, Nicole; Leonard, Didier

    2009-01-01

    This paper deals with the extracellular polymeric substance (EPS) of Proteus mirabilis TJ-1 used as a novel biosorbent to remove dye from aqueous solution in batch systems. As a widely used and hazardous dye, basic blue 54 (BB54) was chosen as the model dye to examine the adsorption performance of the EPS. The effects of pH, initial dye concentration, contact time and temperature on the sorption of BB54 to the EPS were examined. At various initial dye concentrations (50-400 mg/L), the batch sorption equilibrium can be obtained in only 5 min. Kinetic studies suggested that the sorption followed the internal transport mechanism. According to the Langmuir model, the maximum BB54 uptake of 2.005 g/g was obtained. Chemical analysis of the EPS indicated the presence of protein (30.9%, w/w) and acid polysaccharide (63.1%, w/w). Scanning electron microscopy (SEM) images showed that the EPS with a crystal-linear structure was whole enwrapped by adsorbed dye molecules. FTIR spectrum result revealed the presence of adsorbing groups such as carboxyl, hydroxyl and amino groups in the EPS. High-molecular weight of the EPS with more binding-sites and stronger van der Waals forces together with its specific construct leads to the excellent performance of dye adsorption. The EPS shows potential board application as a biosorbent for both environmental protection and dye recovery

  16. Isolation and Purification of Complex II from Proteus Mirabilis Strain ATCC 29245

    Science.gov (United States)

    Shabbiri, Khadija; Ahmad, Waqar; Syed, Quratulain; Adnan, Ahmad

    2010-01-01

    A respiratory complex was isolated from plasma membrane of pathogenic Proteus mirabilis strain ATCC 29245. It was identified as complex II consisting of succinate:quinone oxidoreductase (EC 1.3.5.1) containing single heme b. The complex II was purified by ion-exchange chromatography and gel filtration. The molecular weight of purified complex was 116.5 kDa and it was composed of three subunits with molecular weights of 19 kDa, 29 kDa and 68.5 kDa. The complex II contained 9.5 nmoles of cytochrome b per mg protein. Heme staining indicated that the 19 kDa subunit was cytochrome b. Its reduced form showed absorptions peaks at 557.0, 524.8 and 424.4 nm. The α-band was shifted from 557.0 nm to 556.8 nm in pyridine ferrohemochrome spectrum. The succinate: quinone oxidoreductase activity was found to be high in this microorganism. PMID:24031557

  17. Production of a High Efficiency Microbial Flocculant by Proteus mirabilis TJ-1 Using Compound Organic Wastewater

    Science.gov (United States)

    Zhang, Zhiqiang; Xia, Siqing; Zhang, Jiao

    2010-11-01

    The production of a high efficiency microbial flocculant (MBF) by Proteus mirabilis TJ-1 using compound organic wastewater was investigated. To cut down the cost of the MBF production, several nutritive organic wastewaters were selected to replace glucose and peptone as the carbon source and the nitrogen source in the optimized medium of strain TJ-1, respectively. The compound wastewater of the milk candy and the soybean milk was found to be good carbon source and nitrogen source for this strain to produce MBF. The cost-effective culture medium consists of (per liter): 800 mL wastewater of milk candy, 200 mL wastewater of soybean milk, 0.3 g MgSO4ṡ7 H2O, 5 g K2HPO4, 2 g and KH2PO4, pH 7.0. The economic cost for the MBF production can be cut down over a half by using the developed culture medium. Furthermore, the utilization of the two wastewaters in the preparation of culture medium of strain TJ-1 can not only save their big treatment cost, but also realize their resource reuse.

  18. Dynamical Properties of Transient Spatio-Temporal Patterns in Bacterial Colony of Proteus mirabilis

    Science.gov (United States)

    Watanabe, Kazuhiko; Wakita, Jun-ichi; Itoh, Hiroto; Shimada, Hirotoshi; Kurosu, Sayuri; Ikeda, Takemasa; Yamazaki, Yoshihiro; Matsuyama, Tohey; Matsushita, Mitsugu

    2002-02-01

    Spatio-temporal patterns emerged inside a colony of bacterial species Proteus mirabilis on the surface of nutrient-rich semisolid agar medium have been investigated. We observed various patterns composed of the following basic types: propagating stripe, propagating stripe with fixed dislocation, expanding and shrinking target, and rotating spiral. The remarkable point is that the pattern changes immediately when we alter the position for observation, but it returns to the original if we restore the observing position within a few minutes. We further investigated mesoscopic and microscopic properties of the spatio-temporal patterns. It turned out that whenever the spatio-temporal patterns are observed in a colony, the areas are composed of two superimposed monolayers of elongated bacterial cells. In each area they are aligned almost parallel with each other like a two-dimensional nematic liquid crystal, and move collectively and independently of another layer. It has been found that the observed spatio-temporal patterns are explained as the moiré effect.

  19. Immune enhancement of Taishan Robinia pseudoacacia polysaccharide on recombinant Proteus mirabilis OmpA in chickens.

    Science.gov (United States)

    Zhang, Yongbing; Yang, Shifa; Zhao, Xue; Yang, Ya; Li, Bing; Zhu, Fujie; Zhu, Ruiliang

    2014-09-01

    This study was conducted to evaluate the effects of Taishan Robinia pseudoacacia polysaccharide (TRPPS) on immune responses of chickens immunized with Proteus mirabilis outer membrane protein A (OmpA) recombinant protein vaccine. OmpA was expressed in Pichia pastoris and mixed with TRPPS. 360 chickens were randomly divided into six groups. Groups I to IV were treated with OmpA which contained TRPPS of three different dosages, Freund's adjuvant, respectively. Groups V and VI were treated with pure OmpA and physiological saline, respectively. The data showed that the antibody titers against OmpA, the concentration of IL-2, CD4 +, and CD8 +, T lymphocyte proliferation rate in Group II were significantly higher (P < 0.05) than those in the other groups, little difference in SIgA content was observed among groups I to VI. These results indicated that TRPPS strengthened humoral and cellular immune responses against recombinant OmpA vaccine. Moreover, 200 mg/mL TRPPS showed significance (P < 0.05) compared with Freund's adjuvant. Therefore, TRPPS can be developed into an adjuvant for recombinant subunit vaccine. Copyright © 2014 Elsevier B.V. All rights reserved.

  20. Climate change and potato cropping in the Peruvian Altiplano

    Science.gov (United States)

    Sanabria, J.; Lhomme, J. P.

    2013-05-01

    The potential impacts of climate change on potatoes cropping in the Peruvian highlands (Altiplano) is assessed using climate projections for 2071-2100, obtained from the HadRM3P regional atmospheric model of the Hadley Centre. The atmospheric model is run under two different special report on emission scenarios: high CO2 concentration (A2) and moderate CO2 concentration (B2) for four locations situated in the surroundings of Lake Titicaca. The two main varieties of potato cultivated in the area are studied: the Andean potato ( Solanum tuberosum) and the bitter potato ( Solanum juzepczukii). A simple process-oriented model is used to quantify the climatic impacts on crops cycles and yields by combining the effects of temperature on phenology, of radiation and CO2 on maximum yield and of water balance on yield deficit. In future climates, air temperature systematically increases, precipitation tends to increase at the beginning of the rainy season and slightly decreases during the rest of the season. The direct effects of these climatic changes are earlier planting dates, less planting failures and shorter crop cycles in all the four locations and for both scenarios. Consequently, the harvesting dates occur systematically earlier: roughly in January for the Andean potato instead of March in the current situation and in February for the bitter potato instead of April. Overall, yield deficits will be higher under climate change than in the current climate. There will be a strong negative impact on yields for S. tuberosum (stronger under A2 scenario than under B2); the impact on S. juzepczukii yields, however, appears to be relatively mixed and not so negative.

  1. Identification and characterization of REC66, a Ty1-copia-like retrotransposon in the genome of red flower of Mirabilis jalapa L.

    Directory of Open Access Journals (Sweden)

    Shunri Jiang

    2017-01-01

    Full Text Available Mirabilis jalapa Lis the most commonly grown ornamental species of Mirabilis and is available in a range of brilliant colors. However, genetic research on Mirabilis jalapa Lis limited. Using fluorescent differential display (FDD screening, we report the identification of a novel Ty1-copia-like retrotransposon in the genome of the red flower of Mirabilis jalapa L, and we named it REC66based on its sequence homology to the GAG protein from Ty1-copiaretrotransposon. Using degenerate primers based on the DNA sequence of REC66, a total of fourteen different variants in reverse transcriptase (RT sequence were recovered from the genomic DNA. These RT sequences show a high degree of heterogeneity characterized mainly by deletion mutation; they can be divided into three subfamilies, of which the majority encode defective RT. This is the first report of a Ty1-copiaretrotransposon in Mirabilis jalapa L. The finding could be helpful for the development of new molecular markers for genetic studies, particularly on the origin and evolutionary relationships of M. jalapa L, and the study of Ty1-copiaretrotransposons and plant genome evolution in the genus Mirabilisor family Nyctaginaceae.

  2. Intranasal immunization with fusion protein MrpH·FimH and MPL adjuvant confers protection against urinary tract infections caused by uropathogenic Escherichia coli and Proteus mirabilis.

    Science.gov (United States)

    Habibi, Mehri; Asadi Karam, Mohammad Reza; Shokrgozar, Mohammad Ali; Oloomi, Mana; Jafari, Anis; Bouzari, Saeid

    2015-04-01

    Urinary tract infections (UTIs) caused by Uropathogenic Escherichia coli (UPEC) and Proteus mirabilis are among the most common infections in the world. Currently there are no vaccines available to confer protection against UTI in humans. In this study, the immune responses and protection of FimH of UPEC with MrpH antigen of P. mirabilis in different vaccine formulations with and without MPL adjuvant were assessed. Mice intranasally immunized with the novel fusion protein MrpH·FimH induced a significant increase in IgG and IgA in serum, nasal wash, vaginal wash, and urine samples. Mice immunized with fusion MrpH·FimH also showed a significant boost in cellular immunity. Addition of MPL as the adjuvant enhanced FimH and MrpH specific humoral and cellular responses in both systemic and mucosal samples. Vaccination with MrpH·FimH alone or in combination with MPL showed the highest efficiency in clearing bladder and kidney infections in mice challenged with UPEC and P. mirabilis. These findings may indicate that the protection observed correlates with the systemic, mucosal and cellular immune responses induced by vaccination with these preparations. Our data suggest MrpH·FimH fusion protein with or without MPL as adjuvant could be potential vaccine candidates for elimination of UPEC and P. mirabilis. These data altogether are promising and these formulations are good candidates for elimination of UPEC and P. mirabilis. Copyright © 2014 Elsevier Ltd. All rights reserved.

  3. Role of Proteus mirabilis MR/P fimbriae and flagella in adhesion, cytotoxicity and genotoxicity induction in T24 and Vero cells.

    Science.gov (United States)

    Scavone, Paola; Villar, Silvia; Umpiérrez, Ana; Zunino, Pablo

    2015-06-01

    Proteus mirabilis is frequently associated with complicated urinary tract infections (UTI). It is proposed that several virulence factors are associated with P. mirabilis uropathogenicity. The aim of this work was to elucidate genotoxic and cytotoxic effects mediated by MR/P fimbriae and flagella in eukaryotic cells in vitro. Two cell lines (kidney- and bladder-derived) were infected with a clinical wild-type P. mirabilis strain and an MR/P and a flagellar mutant. We evaluated adhesion, genotoxicity and cytotoxicity by microscopy, comet assay and triple staining technique, respectively. Mutant strains displayed lower adhesion rates than the P. mirabilis wild-type strain and were significantly less effective to induce genotoxic and cytotoxic effects compared to the wild type. We report for the first time that P. mirabilis MR/P fimbriae and flagella mediate genotoxic and cytotoxic effects on eukaryotic cells, at least in in vitro conditions. These results could contribute to design new strategies for the control of UTI. © FEMS 2015. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  4. Cultivation of minor tuber crops in Peru and Bolivia

    Directory of Open Access Journals (Sweden)

    Leena Pietilä

    1988-03-01

    Full Text Available A collection mission of three Andean tuber crops, oca (Oxalis tuberosa, Oxalidaceae, ulluco (Ullucus tuberosus, Basellaceae and añu (Tropaeolum tuberosum, Tropaeolaceae, was carried out in southern Peru, Bolivia and northern Argentina. This article deals with the observations made during this mission. 55 ulluco fields were visited. In general, the fields are small, 240m2 on average, and they are mostly situated on mountain slopes. The fields are fertilized with animal dung; chemical fertilizers are quite rare. In the fields, people work with hoes ang ploughs as they did hundreds of years ago. Mechanization of agriculture would prevent full utilization of the mountainous area of the Andes. Ulluco is usually interplanted with other crops, usually, many forms of ulluco in one field. Because of crop rotation description of the fields is partly valid for the cultivation of other crops, too. Due to drastic climatic variation, cultivation of mixed varieties maybe the best way to guarantee some yield. When results of the investigations are wished to benefit developing countries, knowledge of social, agricultural and environmental factors is of great value.

  5. Origins of The Paleolandslide of Tarapaca (north Chile, Andean Belt)

    Science.gov (United States)

    Darrozes, J.; Pinto, L.; Ingles, J.; Soula, J.-C.; Maire, E.; Courjault-Radé, P.; Hérail, G.

    Landslides are an important and potentially rate-limiting process in the topographic evolution of active orogens like the Andean Belt. Various processes are responsi- ble for triggering landslides, including hillslope baselevel lowering, seismic events (Keefer, 1999); climate changes (Coriminas and Moya, 1999), anthropic effects (Sah and Mazari, 1998) and each of these triggers may be spatially heterogeneous in strength and effectiveness. Present work seeks to identify and constrain the domi- nant mechanism of a Tarapaca PaleoLandslide in order to determine the influence of the overall tectonic uplift of the Andean belt, and the seismicity of the area. The zone of interest is located near Iquique, along the Atacama Desert in the Tarapaca (N. Chile) domain (1955S, 6935W). The climate of the region became dry at 15 Ma (Gregory-Wodzicki, 2000) and remain today one of driest in the world. One of the most significant characteristic of the Atacama Desert landslides is to be located on the western limb of N-S trending flexures. At Tarapaca, the studied landslide is located on the front limb of a fault propagation anticline, the Moquella flexure, of Cenozoic age. The slope in this part increases weakly to reach a value close to 10 degrees. The main scarp of Tarapaca landslide has a length of ≈ 7 km and an elevation close to 200 m. This corresponds to the thickness of an ignimbritic formation which constitutes the load of the landslide. As in most of the Atacama desert, the lateral boundaries of the landslide are two antecedent paleorivers (Suca &Lataguella) which created free edges to the landslide and thus greatly facilitating landsliding. A smaller secondary landslide formed in the foot zone because of the local increase in the slope, which was responsible for the instability of the overlapping mass. The analysis of the safety factor and morphologic features shows that weathering, uplift, water pressure and load are not enough important for creating the slide. It is

  6. Rainfed intensive crop systems

    DEFF Research Database (Denmark)

    Olesen, Jørgen E

    2014-01-01

    This chapter focuses on the importance of intensive cropping systems in contributing to the world supply of food and feed. The impact of climate change on intensive crop production systems is also discussed.......This chapter focuses on the importance of intensive cropping systems in contributing to the world supply of food and feed. The impact of climate change on intensive crop production systems is also discussed....

  7. Zinc uptake contributes to motility and provides a competitive advantage to Proteus mirabilis during experimental urinary tract infection.

    Science.gov (United States)

    Nielubowicz, Greta R; Smith, Sara N; Mobley, Harry L T

    2010-06-01

    Proteus mirabilis, a Gram-negative bacterium, represents a common cause of complicated urinary tract infections in catheterized patients or those with functional or anatomical abnormalities of the urinary tract. ZnuB, the membrane component of the high-affinity zinc (Zn(2+)) transport system ZnuACB, was previously shown to be recognized by sera from infected mice. Since this system has been shown to contribute to virulence in other pathogens, its role in Proteus mirabilis was investigated by constructing a strain with an insertionally interrupted copy of znuC. The znuC::Kan mutant was more sensitive to zinc limitation than the wild type, was outcompeted by the wild type in minimal medium, displayed reduced swimming and swarming motility, and produced less flaA transcript and flagellin protein. The production of flagellin and swarming motility were restored by complementation with znuCB in trans. Swarming motility was also restored by the addition of Zn(2+) to the agar prior to inoculation; the addition of Fe(2+) to the agar also partially restored the swarming motility of the znuC::Kan strain, but the addition of Co(2+), Cu(2+), or Ni(2+) did not. ZnuC contributes to but is not required for virulence in the urinary tract; the znuC::Kan strain was outcompeted by the wild type during a cochallenge experiment but was able to colonize mice to levels similar to the wild-type level during independent challenge. Since we demonstrated a role for ZnuC in zinc transport, we hypothesize that there is limited zinc present in the urinary tract and P. mirabilis must scavenge this ion to colonize and persist in the host.

  8. Emergence of multidrug-resistant Proteus mirabilis in a long-term care facility in Croatia.

    Science.gov (United States)

    Bedenić, Branka; Firis, Nataša; Elveđi-Gašparović, Vesna; Krilanović, Marija; Matanović, Krešimir; Štimac, Iva; Luxner, Josefa; Vraneš, Jasmina; Meštrović, Tomislav; Zarfel, Gernot; Grisold, Andrea

    2016-06-01

    An increased frequency of Proteus mirabilis isolates resistant to expanded-spectrum cephalosporins was observed recently in a long-term care facility in Zagreb (Godan). The aim of this study was the molecular characterization of resistance mechanisms to new cephalosporins in P. mirabilis isolates from this nursing home. Thirty-eight isolates collected from 2013-2015 showing reduced susceptibility to ceftazidime were investigated. Antibiotic susceptibilities were determined by broth microdilution method. Inhibitor-based tests were performed to detect extended-spectrum (ESBLs) and AmpC β-lactamases. AmpC β-lactamases were characterized by polymerase chain reaction (PCR) followed by sequencing of bla ampC genes. Quinolone resistance determinants (qnr genes) were characterized by PCR. Genotyping of the isolates was performed by repetitive element sequence (rep)-PCR and pulsed-field gel electrophoresis (PFGE). Presence of an AmpC β-lactamase was confirmed in all isolates by combined-disk test with phenylboronic acid. All isolates were resistant to amoxicillin alone and combined with clavulanate, cefotaxime, ceftriaxone, cefoxitin, and ciprofloxacin; but susceptible to cefepime, imipenem, and meropenem. PCR followed by sequencing using primers targeting bla ampc genes revealed CMY-16 β-lactamase in all but one strain. Bla cmy-16 was carried by a non-conjugative plasmid which did not belong to any known plasmid-based replicon typing (PBRT) group. Rep-PCR identified one large clone consisting of 15 isolates, three pairs or related isolates, one triplet, and four singletons. PFGE confirmed the clonality of the isolates. This is the first report of multidrug resistant P. mirabilis in a nursing home in Croatia. Cephalosporin resistance was due to plasmid-mediated AmpC β-lactamase CMY-16.

  9. The Self-Identity Protein IdsD Is Communicated between Cells in Swarming Proteus mirabilis Colonies.

    Science.gov (United States)

    Saak, Christina C; Gibbs, Karine A

    2016-12-15

    Proteus mirabilis is a social bacterium that is capable of self (kin) versus nonself recognition. Swarming colonies of this bacterium expand outward on surfaces to centimeter-scale distances due to the collective motility of individual cells. Colonies of genetically distinct populations remain separate, while those of identical populations merge. Ids proteins are essential for this recognition behavior. Two of these proteins, IdsD and IdsE, encode identity information for each strain. These two proteins bind in vitro in an allele-restrictive manner. IdsD-IdsE binding is correlated with the merging of populations, whereas a lack of binding is correlated with the separation of populations. Key questions remained about the in vivo interactions of IdsD and IdsE, specifically, whether IdsD and IdsE bind within single cells or whether IdsD-IdsE interactions occur across neighboring cells and, if so, which of the two proteins is exchanged. Here we demonstrate that IdsD must originate from another cell to communicate identity and that this nonresident IdsD interacts with IdsE resident in the recipient cell. Furthermore, we show that unbound IdsD in recipient cells does not cause cell death and instead appears to contribute to a restriction in the expansion radius of the swarming colony. We conclude that P. mirabilis communicates IdsD between neighboring cells for nonlethal kin recognition, which suggests that the Ids proteins constitute a type of cell-cell communication. We demonstrate that self (kin) versus nonself recognition in P. mirabilis entails the cell-cell communication of an identity-encoding protein that is exported from one cell and received by another. We further show that this intercellular exchange affects swarm colony expansion in a nonlethal manner, which adds social communication to the list of potential swarm-related regulatory factors. Copyright © 2016, American Society for Microbiology. All Rights Reserved.

  10. Changing Hydrology in Glacier-fed High Altitude Andean Peatbogs

    Science.gov (United States)

    Slayback, D. A.; Yager, K.; Baraer, M.; Mohr, K. I.; Argollo, J.; Wigmore, O.; Meneses, R. I.; Mark, B. G.

    2012-12-01

    Montane peatbogs in the glacierized Andean highlands of Peru and Bolivia provide critical forage for camelids (llama and alpaca) in regionally extensive pastoral agriculture systems. During the long dry season, these wetlands often provide the only available green forage. A key question for the future of these peatbog systems, and the livelihoods they support, is the impact of climate change and glacier recession on their hydrology, and thus forage production. We have already documented substantial regional glacier recession, of, on average, approximately 30% of surface area over the past two decades. As glaciers begin to retreat under climate change, there is initially a period of increased meltwater outflow, culminating in a period of "peak water", and followed by a continual decline in outflows. Based on previous work, we know that some glaciers in the region have already passed peak water conditions, and are now declining. To better understand the impacts of these processes on peatbog hydrology and productivity, we have begun collecting a variety of surface data at several study sites in both Bolivia and Peru. These include precipitation, stream flow, water levels, water chemistry and isotope analyses, and peatbog biodiversity and biomass. These measurements will be used in conjunction with a regional model driven by satellite data to predict likely future impacts. We will present the results from these initial surface measurements, and an overview of satellite datasets to be used in the regional model.

  11. Polyhydroxyalkanoate biosynthesis by oxalotrophic bacteria from high Andean soil

    Directory of Open Access Journals (Sweden)

    Roger David Castillo-Arteaga

    2018-02-01

    Full Text Available Oxalate is a highly oxidized organic acid anion used as a carbon and energy source by oxalotrophic bacteria. Oxalogenic plants convert atmospheric CO2 into oxalic acid and oxalic salts. Oxalate-salt formation acts as a carbon sink in terrestrial ecosystems via the oxalate-carbonate pathway (OCP. Oxalotrophic bacteria might be implicated in other carbon-storage processes, including the synthesis of polyhydroxyalkanoates (PHAs. More recently, a variety of bacteria from the Andean region of Colombia in Nariño have been reported for their PHA-producing abilities. These species can degrade oxalate and participate in the oxalate-carbonate pathway. The aim of this study was to isolate and characterize oxalotrophic bacteria with the capacity to accumulate PHA biopolymers. Plants of the genus Oxalis were collected and bacteria were isolated from the soil adhering to the roots. The isolated bacterial strains were characterized using biochemical and molecular biological methods. The consumption of oxalate in culture was quantified, and PHA production was monitored in batch fermentation. The polymeric composition was characterized using gas chromatography. Finally, a biosynthetic pathway based on our findings and on those from published sources is proposed. Strains of Bacillus spp. and Serratia sp. were found to metabolize calcium oxalate and synthesize PHA.

  12. The epidemiology and control of leishmaniasis in Andean countries

    Directory of Open Access Journals (Sweden)

    Davies Clive Richard

    2000-01-01

    Full Text Available This paper reviews the current knowledge of leishmaniasis epidemiology in Venezuela, Colombia, Ecuador, Peru, and Bolivia. In all 5 countries leishmaniasis is endemic in both the Andean highlands and the Amazon basin. The sandfly vectors belong to subgenera Helcocyrtomyia, Nyssomiya, Lutzomyia, and Psychodopygus, and the Verrucarum group. Most human infections are caused by Leishmania in the Viannia subgenus. Human Leishmania infections cause cutaneous lesions, with a minority of L. (Viannia infections leading to mucocutaneous leishmaniasis. Visceral leishmaniasis and diffuse cutaneous leishmaniasis are both rare. In each country a significant proportion of Leishmania transmission is in or around houses, often close to coffee or cacao plantations. Reservoir hosts for domestic transmission cycles are uncertain. The paper first addresses the burden of disease caused by leishmaniasis, focusing on both incidence rates and on the variability in symptoms. Such information should provide a rational basis for prioritizing control resources, and for selecting therapy regimes. Secondly, we describe the variation in transmission ecology, outlining those variables which might affect the prevention strategies. Finally, we look at the current control strategies and review the recent studies on control.

  13. From Subordinate Marker to Discourse Marker: que in Andean Spanish

    Directory of Open Access Journals (Sweden)

    Anna María Escobar

    2005-06-01

    Full Text Available This paper proposes an analysis of a redundant use of que ('that' found in Andean Spanish as an expression which has undergone a grammaticalization process. Evidence suggests that the function of que as subordinate marker is much more generalized in this variety than in other dialects of Spanish. que is found to be used as a marker introducing both nominal and adjectival clauses, suggesting that adjectival subordinates behave as nominal subordinates in this variety of Spanish. An intrusive que appears in restricted syntactic and semantic contexts with clauses that have nominal and adjectival functions, and even appears replacing adverbial expressions in some adverbial subordinates (temporal, spatial, and manner. Furthermore, it is found to be sensitive to the degree of the argument’s thematic/semantic function in the subordinate clause. In particular, it seems to occur more often with low-agency arguments in adjectival and nominal contexts, and, in nominal subordinates, tends to appear with a restricted set of epistemic and evidential main verbs (e.g. creer 'to believe', saber 'to know', decir 'to say'. The analysis suggests that que has developed a new function in this variety of Spanish, namely, one of indicating that the information contained in the subordinate clause does not constitute background information (as would be expected in non-contact varieties of Spanish but instead contains information relevant to the discourse.

  14. Phoretic mites identified on andean hummingbirds (Trochilidae of Caldas, Colombia

    Directory of Open Access Journals (Sweden)

    Natalia López-Orozco

    Full Text Available Within the bird-plant-mite system, the relationship between hummingbirds, flowers, and mites remains poorly understood. In this study, we evaluated the degree of association between nasal mites and eight species of Andean hummingbirds in Colombia (Amazilia saucerrottei,A. tzacatl, Chalybura buffonii,Chlorostilbon mellisugus, Florisuga mellivora, Glaucis hirsutus, Phaethornis guy and P. striigularis. Over a five-month period (trapping effort 360 hours/month, a total of 178 birds were captured, from which 81 mite specimens were collected and identified as belonging to three genera (Proctolaelaps, Rhinoseius andTropicoseius spanning eleven species. This is the first report of its kind from Colombia on the identification of the mite speciesP. rabulatus, R. luteyni, R. rafinskii, T. berryi, T. colwelli, T. erro and T. uniformisand the first record of P. guy as phoretic host forProctolaelaps rabulatus. Morphological characteristics (length of the dorsal plate, width of the dorsal plate and setae z5 length alone failed to distinguish between mite species. The ecologic impact of this relationship on flowers with respect to nectar and pollen availability and the effect of mites on pollination by hummingbirds needs to be determined.

  15. Phoretic mites identified on Andean hummingbirds (Trochilidae) of Caldas, Colombia.

    Science.gov (United States)

    López-Orozco, Natalia; Cañón-Franco, William Alberto

    2013-01-01

    Within the bird-plant-mite system, the relationship between hummingbirds, flowers, and mites remains poorly understood. In this study, we evaluated the degree of association between nasal mites and eight species of Andean hummingbirds in Colombia (Amazilia saucerrottei, A. tzacatl, Chalybura buffonii, Chlorostilbon mellisugus, Florisuga mellivora, Glaucis hirsutus, Phaethornis guy and P. striigularis). Over a five-month period (trapping effort 360 hours/month), a total of 178 birds were captured, from which 81 mite specimens were collected and identified as belonging to three genera (Proctolaelaps, Rhinoseius and Tropicoseius) spanning eleven species. This is the first report of its kind from Colombia on the identification of the mite species P. rabulatus, R. luteyni, R. rafinskii, T. berryi, T. colwelli, T. erro and T. uniformis and the first record of P. guy as phoretic host for Proctolaelaps rabulatus. Morphological characteristics (length of the dorsal plate, width of the dorsal plate and setae z5 length) alone failed to distinguish between mite species. The ecologic impact of this relationship on flowers with respect to nectar and pollen availability and the effect of mites on pollination by hummingbirds needs to be determined.

  16. The epidemiology and control of leishmaniasis in Andean countries

    Directory of Open Access Journals (Sweden)

    Clive Richard Davies

    Full Text Available This paper reviews the current knowledge of leishmaniasis epidemiology in Venezuela, Colombia, Ecuador, Peru, and Bolivia. In all 5 countries leishmaniasis is endemic in both the Andean highlands and the Amazon basin. The sandfly vectors belong to subgenera Helcocyrtomyia, Nyssomiya, Lutzomyia, and Psychodopygus, and the Verrucarum group. Most human infections are caused by Leishmania in the Viannia subgenus. Human Leishmania infections cause cutaneous lesions, with a minority of L. (Viannia infections leading to mucocutaneous leishmaniasis. Visceral leishmaniasis and diffuse cutaneous leishmaniasis are both rare. In each country a significant proportion of Leishmania transmission is in or around houses, often close to coffee or cacao plantations. Reservoir hosts for domestic transmission cycles are uncertain. The paper first addresses the burden of disease caused by leishmaniasis, focusing on both incidence rates and on the variability in symptoms. Such information should provide a rational basis for prioritizing control resources, and for selecting therapy regimes. Secondly, we describe the variation in transmission ecology, outlining those variables which might affect the prevention strategies. Finally, we look at the current control strategies and review the recent studies on control.

  17. Growth responses and cadmium accumulation of Mirabilis jalapa L. under interaction between cadmium and phosphorus

    International Nuclear Information System (INIS)

    Yu Zhiguo; Zhou Qixing

    2009-01-01

    Based on the identification of Mirabilis jalapa L. as a new Cd-hyperaccumulating ornamental, the growth response of the plant to interaction between cadmium (Cd) and phosphorus (P), its effect on the Cd accumulation in the species and relevant mechanisms were further investigated by the pot-culture experiment with chemical analyses and the X-ray absorption near edge structure spectra (XANES). It showed that the leaf, shoot and root biomass (as dry matter) increased with an increase in P supplies from 20 to 100 mg kg -1 at various tested Cd levels except 10 mg kg -1 . The Cd accumulation in the leaves and shoots significantly decreased with increasing P concentrations from 20 to 500 mg kg -1 at the Cd concentrations from 10 to 50 mg kg -1 , except the Cd level at 100 mg kg -1 . It was also found that the translocation factor of Cd in M. jalapa L. reached the maximum at different tested Cd levels when the concentration of added P was 100 mg kg -1 , but the bioaccumulation factor of Cd decreased with increasing P. This changing law may be responsible for the mechanism of P immobilizing Cd. The investigation using the P-K edge XANES showed the spectra of adsorbed phosphate in the shoots exhibited a stronger white-line peak than that in the leaves and roots, and the oscillation near 2165 eV was more intense. Besides, the P-K edge XANES spectra for M. jalapa L. indicated P may exist as Cd-phosphate. Thus, it can be inferred that the addition of P at appropriate contents may be a useful approach to enhance the plant growth and to immobilize Cd in the Cd-contaminated soil. Furthermore, P and Cd may form a deposit in plants to tolerate Cd toxicity for reducing the degree of the structural damage of the plant.

  18. Anaerobic Respiration Using a Complete Oxidative TCA Cycle Drives Multicellular Swarming in Proteus mirabilis

    Science.gov (United States)

    Alteri, Christopher J.; Himpsl, Stephanie D.; Engstrom, Michael D.; Mobley, Harry L. T.

    2012-01-01

    ABSTRACT Proteus mirabilis rapidly migrates across surfaces using a periodic developmental process of differentiation alternating between short swimmer cells and elongated hyperflagellated swarmer cells. To undergo this vigorous flagellum-mediated motility, bacteria must generate a substantial proton gradient across their cytoplasmic membranes by using available energy pathways. We sought to identify the link between energy pathways and swarming differentiation by examining the behavior of defined central metabolism mutants. Mutations in the tricarboxylic acid (TCA) cycle (fumC and sdhB mutants) caused altered patterns of swarming periodicity, suggesting an aerobic pathway. Surprisingly, the wild-type strain swarmed on agar containing sodium azide, which poisons aerobic respiration; the fumC TCA cycle mutant, however, was unable to swarm on azide. To identify other contributing energy pathways, we screened transposon mutants for loss of swarming on sodium azide and found insertions in the following genes that involved fumarate metabolism or respiration: hybB, encoding hydrogenase; fumC, encoding fumarase; argH, encoding argininosuccinate lyase (generates fumarate); and a quinone hydroxylase gene. These findings validated the screen and suggested involvement of anaerobic electron transport chain components. Abnormal swarming periodicity of fumC and sdhB mutants was associated with the excretion of reduced acidic fermentation end products. Bacteria lacking SdhB were rescued to wild-type pH and periodicity by providing fumarate, independent of carbon source but dependent on oxygen, while fumC mutants were rescued by glycerol, independent of fumarate only under anaerobic conditions. These findings link multicellular swarming patterns with fumarate metabolism and membrane electron transport using a previously unappreciated configuration of both aerobic and anaerobic respiratory chain components. PMID:23111869

  19. Genetic diversity of the Andean tuber-bearing species, oca (Oxalis tuberosa Mol.), investigated by inter-simple sequence repeats.

    Science.gov (United States)

    Pissard, A; Ghislain, M; Bertin, P

    2006-01-01

    The Andean tuber-bearing species, Oxalis tuberosa Mol., is a vegetatively propagated crop cultivated in the uplands of the Andes. Its genetic diversity was investigated in the present study using the inter-simple sequence repeat (ISSR) technique. Thirty-two accessions originating from South America (Argentina, Bolivia, Chile, and Peru) and maintained in vitro were chosen to represent the ecogeographic diversity of its cultivation area. Twenty-two primers were tested and 9 were selected according to fingerprinting quality and reproducibility. Genetic diversity analysis was performed with 90 markers. Jaccard's genetic distance between accessions ranged from 0 to 0.49 with an average of 0.28 +/- 0.08 (mean +/- SD). Dendrogram (UPGMA (unweighted pair-group method with arithmetic averaging)) and factorial correspondence analysis (FCA) showed that the genetic structure was influenced by the collection site. The two most distant clusters contained all of the Peruvian accessions, one from Bolivia, none from Argentina or Chile. Analysis by country revealed that Peru presented the greatest genetic distances from the other countries and possessed the highest intra-country genetic distance (0.30 +/- 0.08). This suggests that the Peruvian oca accessions form a distinct genetic group. The relatively low level of genetic diversity in the oca species may be related to its predominating reproduction strategy, i.e., vegetative propagation. The extent and structure of the genetic diversity of the species detailed here should help the establishment of conservation strategies.

  20. Distinct Residues Contribute to Motility Repression and Autoregulation in the Proteus mirabilis Fimbria-Associated Transcriptional Regulator AtfJ.

    Science.gov (United States)

    Bode, Nadine J; Chan, Kun-Wei; Kong, Xiang-Peng; Pearson, Melanie M

    2016-08-01

    Proteus mirabilis contributes to a significant number of catheter-associated urinary tract infections, where coordinated regulation of adherence and motility is critical for ascending disease progression. Previously, the mannose-resistant Proteus-like (MR/P) fimbria-associated transcriptional regulator MrpJ has been shown to both repress motility and directly induce the transcription of its own operon; in addition, it affects the expression of a wide range of cellular processes. Interestingly, 14 additional mrpJ paralogs are included in the P. mirabilis genome. Looking at a selection of MrpJ paralogs, we discovered that these proteins, which consistently repress motility, also have nonidentical functions that include cross-regulation of fimbrial operons. A subset of paralogs, including AtfJ (encoded by the ambient temperature fimbrial operon), Fim8J, and MrpJ, are capable of autoinduction. We identified an element of the atf promoter extending from 487 to 655 nucleotides upstream of the transcriptional start site that is responsive to AtfJ, and we found that AtfJ directly binds this fragment. Mutational analysis of AtfJ revealed that its two identified functions, autoregulation and motility repression, are not invariably linked. Residues within the DNA-binding helix-turn-helix domain are required for motility repression but not necessarily autoregulation. Likewise, the C-terminal domain is dispensable for motility repression but is essential for autoregulation. Supported by a three-dimensional (3D) structural model, we hypothesize that the C-terminal domain confers unique regulatory capacities on the AtfJ family of regulators. Balancing adherence with motility is essential for uropathogens to successfully establish a foothold in their host. Proteus mirabilis uses a fimbria-associated transcriptional regulator to switch between these antagonistic processes by increasing fimbrial adherence while simultaneously downregulating flagella. The discovery of multiple

  1. St. Catherine of Siena (1347-1380 AD): one of the earliest historic cases of altered gustatory perception in anorexia mirabilis.

    Science.gov (United States)

    Galassi, Francesco M; Bender, Nicole; Habicht, Michael E; Armocida, Emanuele; Toscano, Fabrizio; Menassa, David A; Cerri, Matteo

    2018-05-01

    St. Catherine of Siena suffered from an extreme form of holy fasting, a condition classified as anorexia mirabilis (also known as inedia prodigiosa). Historical and medical scholarships alike have drawn a comparison between this primaeval type of anorexia with a relatively common form of eating disorder among young women in the modern world, anorexia nervosa. St. Catherine's condition was characterised by a disgust for sweet taste, a condition also described in anorexia nervosa, and characterised by specific neurophysiological changes in the brain. St. Catherine's case may be considered one of the oldest veritable descriptions of altered gustation (dysgeusia). Moreover, a more compelling neurophysiological similarity between anorexia mirabilis and anorexia nervosa may be proposed.

  2. Use of spatial capture–recapture to estimate density of Andean bears in northern Ecuador

    Science.gov (United States)

    Molina, Santiago; Fuller, Angela K.; Morin, Dana J.; Royle, J. Andrew

    2017-01-01

    The Andean bear (Tremarctos ornatus) is the only extant species of bear in South America and is considered threatened across its range and endangered in Ecuador. Habitat loss and fragmentation is considered a critical threat to the species, and there is a lack of knowledge regarding its distribution and abundance. The species is thought to occur at low densities, making field studies designed to estimate abundance or density challenging. We conducted a pilot camera-trap study to estimate Andean bear density in a recently identified population of Andean bears northwest of Quito, Ecuador, during 2012. We compared 12 candidate spatial capture–recapture models including covariates on encounter probability and density and estimated a density of 7.45 bears/100 km2 within the region. In addition, we estimated that approximately 40 bears used a recently named Andean bear corridor established by the Secretary of Environment, and we produced a density map for this area. Use of a rub-post with vanilla scent attractant allowed us to capture numerous photographs for each event, improving our ability to identify individual bears by unique facial markings. This study provides the first empirically derived density estimate for Andean bears in Ecuador and should provide direction for future landscape-scale studies interested in conservation initiatives requiring spatially explicit estimates of density.

  3. Seed production differences of the Andean oak Quercus Humboldtii Bonpl. in two Andean forests of the Colombian Eastern Cordillera

    International Nuclear Information System (INIS)

    Gonzalez Melo, Andres; Parrado Rosselli Angela

    2010-01-01

    Knowledge of mechanisms of tree species reproduction under natural situations including fruit and seed production patterns is very important for forest management strategies. Considering the influence of abiotic factors such as soil characteristics, humidity and rainfall on fruiting phenology, we studied fruit production patterns of the Andean oak (Quercus humboldtii: Fagaceae) in two forest sites of the Colombian Eastern Cordillera (Cachalu and Patios Altos), under contrasting environmental conditions. At both sites, we monitored monthly fruit production of 15 trees in Cachalu and 11 in Patios Altos using fruit/seed traps placed under the tree crowns. In each site soil cores were extracted below the litter layer 20 cm depth, and soil characteristics and nutrients were analyzed. In general, trees in Cachalu produced more fruits than in Patios Altos, as well as mean fruit mass (wet and dry weight) was significantly higher in Cachalu. At both sites, oak fruiting peaked from April to May, when the highest rainfall occurs. We found positive correlations between fruit production and rainfall one month prior. High phosphorus (P) and potassium (K) were the main variables for explaining the high production. In contrast, high aluminum (Al) contents explained the low production found in Patios Altos. We discuss the importance of including fruit production for oak management strategies, such as restoration and reforestation programs.

  4. Sediment budget in the Ucayali River basin, an Andean tributary of the Amazon River

    Directory of Open Access Journals (Sweden)

    W. Santini

    2015-03-01

    Full Text Available Formation of mountain ranges results from complex coupling between lithospheric deformation, mechanisms linked to subduction and surface processes: weathering, erosion, and climate. Today, erosion of the eastern Andean cordillera and sub-Andean foothills supplies over 99% of the sediment load passing through the Amazon Basin. Denudation rates in the upper Ucayali basin are rapid, favoured by a marked seasonality in this region and extreme precipitation cells above sedimentary strata, uplifted during Neogene times by a still active sub-Andean tectonic thrust. Around 40% of those sediments are trapped in the Ucayali retro-foreland basin system. Recent advances in remote sensing for Amazonian large rivers now allow us to complete the ground hydrological data. In this work, we propose a first estimation of the erosion and sedimentation budget of the Ucayali River catchment, based on spatial and conventional HYBAM Observatory network.

  5. Paired Magmatic-Metallogenic Belts in Myanmar - an Andean Analogue?

    Science.gov (United States)

    Gardiner, Nicholas; Robb, Laurence; Searle, Michael; Morley, Christopher

    2015-04-01

    contrasting minerals endowment. The Mogok-Mandalay-Mergui (MMM) Belt hosts crustal-melt S-type granites with significant tin-tungsten mineralization, and contains the historically major tungsten deposit of Mawchi. The Wuntho-Popa Arc comprises I-type granites and granodiorites with porphyry-type copper-gold and epithermal gold mineralization, and includes the world-class Monywa copper mine. Recent U-Pb radiometric age dating has shown the potential for the two belts to be both active from the Late Cretaceous to Eocene. The spatial juxtaposition of these two sub-parallel belts, the implication of contemporary magmatism, and their distinct but consistent metallogenic endowment bears strong similarities to the metallogenic belts of the South American Cordillera. Here we investigate whether they together represent the magmatic and metallogenic expression of an Andean-type setting in Myanmar during the subduction of Neo-Tethys. In this analogue the Wuntho-Popa Arc represents a proximal I-type magmatic belt sited immediately above the eastwards-verging Neo-Tethys subduction zone. Exhibiting porphyry-type copper-gold and epithermal gold mineralization, this would therefore be the Myanmar equivalent of the Andean coastal copper belts. Conversely, the parallel MMM Belt, comprised of more distal crustal-melt S-type tin granites, would have an analogue in the Bolivian tin belt.

  6. Efektivitas SNEDDS Ekstrak Kulit Manggis Terhadap Bakteri P. mirabilis dan S. epidermidis yang Terdapat pada Ulkus Diabetik

    Directory of Open Access Journals (Sweden)

    Rafika Sari

    2016-12-01

    Full Text Available Mangosteen rind has the ability to conduct antibacterial activity. Under the development relating to drug delivery system, SNEDDS were known to boost the penetration of active compound. The aim of this research was to compare the effectiveness of the antibacterial activity at both SNEDDS preparation and the ethanol extract of mangosteen rind as the antibacteria against the bacteria that cause diabetic ulcers with the most prevalence whether it was Gram positive or Gram negative bacteria like Proteus mirabilis and Staphylococcus epidermidis. The research started by maceration process with the crude extract as the result continued with the phytochemical screening which subsequently prepared as SNEDDS preparations. The SNEDDS preparation prepared earlier then analyzed to see the content of the active compound using spectrophotometer UV-Vis and the measuring of antibacteria activity with bacteria growth inhibitory parameter at ethanol extract SNEDDS preparations of mangosteen rind then compared to ciprofloxacin and were analyzed the data afterwards using ANOVA. The results shows that SNEDDS preparation of mangosteen extract have an activity against both typed of bacteria that cause diabetic ulcers shows by significant differences between both group which is P. mirabilis bacteria group and S. epidermidis bacteria group in ANOVA test with significant number of 0,000 and shows significant differences between both group.

  7. Proteus Mirabilis Bacteria Biosensor Development Based on Modified Gold Electrode with 4-Carboxyphenyl Diazonium Salts for Heavy Metals Toxicity Detection

    Directory of Open Access Journals (Sweden)

    Yosra BRAHAM

    2014-05-01

    Full Text Available In this work we describe a new biosensor for heavy metals detection, based on the immobilization of bacteria, Proteus mirabilis on gold electrode modified with aryl electrografting film. To enhance the stability of the biosystem, additional materials were used such as functionalized Fe3O4 nanoparticles (NPs, cationic (PAH, anionic (PSS polyelectrolytes, Bovine Serum Albumin (BSA and glutaraldehyde as a cross-linking agent. Before the immobilization step, the activity of Proteus mirabilis bacteria in the presence of heavy metals ions was attempted using the ion ammonium selective electrodes (ISEs. The modication of the gold electrodes with the electrochemical reduction of 4- carboxyphenyl diazonium salts to form stable layers for sensing applications was characterized by cyclic voltammetry and chronoamperometry measurements. The adhesion of the bacteria cell on gold electrode was evaluated using contact angle measurements. The immobilized bacteria-metal interaction was evaluated using the electrochemical impedance spectroscopy (EIS measurements. A notable effect of metal on the bacteria activity is observed in the concentration range from 10-3 to 1 µM and from 1µM to 1nM for Co2+, Cd2+, Cu2+ and Hg2+, respectively.

  8. Arginine promotes Proteus mirabilis motility and fitness by contributing to conservation of the proton gradient and proton motive force.

    Science.gov (United States)

    Armbruster, Chelsie E; Hodges, Steven A; Smith, Sara N; Alteri, Christopher J; Mobley, Harry L T

    2014-10-01

    Swarming contributes to Proteus mirabilis pathogenicity by facilitating access to the catheterized urinary tract. We previously demonstrated that 0.1-20 mmol/L arginine promotes swarming on normally nonpermissive media and that putrescine biosynthesis is required for arginine-induced swarming. We also previously determined that arginine-induced swarming is pH dependent, indicating that the external proton concentration is critical for arginine-dependent effects on swarming. In this study, we utilized survival at pH 5 and motility as surrogates for measuring changes in the proton gradient (ΔpH) and proton motive force (μH(+) ) in response to arginine. We determined that arginine primarily contributes to ΔpH (and therefore μH(+) ) through the action of arginine decarboxylase (speA), independent of the role of this enzyme in putrescine biosynthesis. In addition to being required for motility, speA also contributed to fitness during infection. In conclusion, consumption of intracellular protons via arginine decarboxylase is one mechanism used by P. mirabilis to conserve ΔpH and μH(+) for motility. © 2014 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.

  9. Dissemination of a Multidrug-Resistant VIM-1- and CMY-99-Producing Proteus mirabilis Clone in Bulgaria.

    Science.gov (United States)

    Markovska, Rumyana; Schneider, Ines; Keuleyan, Emma; Ivanova, Dobrinka; Lesseva, Magdalena; Stoeva, Temenuga; Sredkova, Mariya; Bauernfeind, Adolf; Mitov, Ivan

    2017-04-01

    The aim of this study was to analyze the beta-lactamases and the molecular epidemiology of 19 clinically significant isolates of Proteus mirabilis with decreased susceptibility to imipenem, which have been collected from seven hospitals, located in different Bulgarian towns (Sofia, Varna, and Pleven). The isolates were obtained from blood, urine, tracheal and wound specimens. One additional isolate from hospital environment was included. Susceptibility testing, conjugation experiments, and plasmid replicon typing were carried out. Beta-lactamases were characterized by isoelectric focusing, PCR, and sequencing. Clonal relatedness was investigated by RAPD and PFGE. Integron mapping was performed by PCR and sequencing. All isolates showed a multidrug-resistance profile, but remained susceptible to piperacillin/tazobactam, cefepime, meropenem, and fosfomycin. They produced identical beta-lactamases, namely: TEM-1, VIM-1, and CMY-99. PCR mapping revealed that the bla VIM-1 gene was part of a class 1 integron that additionally included the aac(6')-I, dhfrA1, and ant(3″)-Ia genes. In addition, 17 of the isolates carried the armA gene. Conjugation experiments and plasmid replicon typing were unsuccessful. The isolates were clonally related according to RAPD and PFGE typing. This study reveals the nationwide distribution of a multidrug-resistant P. mirabilis clone producing VIM-1 and CMY-99 along with the presence of different aminoglycoside resistance mechanisms.

  10. Katalysis: helping Andean farmers adapt to climate change

    NARCIS (Netherlands)

    Sherwood, S.G.; Bentley, J.

    2009-01-01

    Recent studies of global climate change paint a bleak picture for the Andes. Researchers have proposed expert-led solutions, such as improved climatic modelling and forecasting, and the breeding of drought-tolerant crop varieties. In this article, the authors argue that farmers need to shape the

  11. [Structural recovering in Andean successional forests from Porce (Antioquia, Colombia)].

    Science.gov (United States)

    Yepes, Adriana P; del Valle, Jorge I; Jaramillo, Sandra L; Orrego, Sergio A

    2010-03-01

    Places subjected to natural or human disturbance can recover forest through an ecological process called secondary succession. Tropical succession is affected by factors such as disturbances, distance from original forest, surface configuration and local climate. These factors determine the composition of species and the time trend of the succession itself. We studied succession in soils used for cattle ranching over various decades in the Porce Region of Colombia (Andean Colombian forests). A set of twenty five permanent plots was measured, including nine plots (20 x 50 m) in primary forests and sixteen (20 x 25 m) in secondary forests. All trees with diameter > or =1.0 cm were measured. We analyzed stem density, basal area, above-ground biomass and species richness, in a successional process of ca. 43 years, and in primary forests. The secondary forests' age was estimated in previous studies, using radiocarbon dating, aerial photographs and a high-resolution satellite image analysis (7 to >43 years). In total, 1,143 and 1,766 stems were measured in primary and secondary forests, respectively. Basal area (5.7 to 85.4 m2 ha(-1)), above-ground biomass (19.1 to 1,011.5 t ha(-1)) and species richness (4 to 69) directly increased with site age, while steam density decreased (3,180 to 590). Diametric distributions were "J-inverted" for primary forests and even-aged size-class structures for secondary forests. Three species of palms were abundant and exclusive in old secondary forests and primary forests: Oenocarpus mapora, Euterpe precatoria and Oenocarpus bataua. These palms happened in cohorts after forest disturbances. Secondary forest structure was 40% in more than 43 years of forest succession and indicate that many factors are interacting and affecting the forests succession in the area (e.g. agriculture, cattle ranching, mining, etc.).

  12. Social inequality and child malnutrition in four Andean countries.

    Science.gov (United States)

    Larrea, Carlos; Freire, Wilma

    2002-01-01

    To analyze the effects of socioeconomic, regional, and ethnic conditions on chronic malnutrition in four Andean countries of South America: Bolivia, Colombia, Ecuador, and Peru. The study was based on Demographic and Health Surveys (DHS) for Colombia (1995), Peru (1996), and Bolivia (1997), and on a Living Standard Measurement Survey for Ecuador (1998). We developed an index of household socioeconomic status using categorical principal components analysis. We broke down the prevalence of stunting by socioeconomic status (SES), ethnicity, place of residence (large cities, small cities, towns, and countryside), and region (highland region versus other areas of the country). We applied smoothed regression curves and linear functions to analyze SES effects on stunting, with specific models for Bolivia, Ecuador, and Peru. Bolivia, Ecuador, and Peru have similar characteristics, with high stunting prevalences overall; higher stunting prevalences in their highland areas, particularly among indigenous populations; and strong socioeconomic disparities. Colombia, in contrast, has a lower stunting prevalence and smaller regional disparities. The socioeconomic gradient of stunting is strong in all four countries, with prevalence rates in the poorest deciles at least three times as high as those in the top decile. The sharp contrast between the conditions found in Bolivia, Ecuador, and Peru and those in Colombia may be the result of specific ethnic factors affecting indigenous groups; a particular diet profile in the highland areas, with low protein and micronutrient intake; and differences in the long-term economic and social development paths that the countries have taken. Along with the strong socioeconomic gradient in all the countries, the weight of ethnic and regional factors suggests the need to reduce inequality as well as to comprehensively improve education and housing, better target health and nutrition programs, and implement participatory programs integrated into

  13. Social inequality and child malnutrition in four Andean countries

    Directory of Open Access Journals (Sweden)

    Carlos Larrea

    2002-06-01

    Full Text Available Objective. To analyze the effects of socioeconomic, regional, and ethnic conditions on chronic malnutrition in four Andean countries of South America: Bolivia, Colombia, Ecuador, and Peru. Methods. The study was based on Demographic and Health Surveys (DHS for Colombia (1995, Peru (1996, and Bolivia (1997, and on a Living Standard Measurement Survey for Ecuador (1998. We developed an index of household socioeconomic status using categorical principal components analysis. We broke down the prevalence of stunting by socioeconomic status (SES, ethnicity, place of residence (large cities, small cities, towns, and countryside, and region (highland region versus other areas of the country. We applied smoothed regression curves and linear functions to analyze SES effects on stunting, with specific models for Bolivia, Ecuador, and Peru. Results. Bolivia, Ecuador, and Peru have similar characteristics, with high stunting prevalences overall; higher stunting prevalences in their highland areas, particularly among indigenous populations; and strong socioeconomic disparities. Colombia, in contrast, has a lower stunting prevalence and smaller regional disparities. The socioeconomic gradient of stunting is strong in all four countries, with prevalence rates in the poorest deciles at least three times as high as those in the top decile. Discussion. The sharp contrast between the conditions found in Bolivia, Ecuador, and Peru and those in Colombia may be the result of specific ethnic factors affecting indigenous groups; a particular diet profile in the highland areas, with low protein and micronutrient intake; and differences in the long-term economic and social development paths that the countries have taken. Along with the strong socioeconomic gradient in all the countries, the weight of ethnic and regional factors suggests the need to reduce inequality as well as to comprehensively improve education and housing, better target health and nutrition programs

  14. Diverging responses of tropical Andean biomes under future climate conditions.

    Directory of Open Access Journals (Sweden)

    Carolina Tovar

    Full Text Available Observations and projections for mountain regions show a strong tendency towards upslope displacement of their biomes under future climate conditions. Because of their climatic and topographic heterogeneity, a more complex response is expected for biodiversity hotspots such as tropical mountain regions. This study analyzes potential changes in the distribution of biomes in the Tropical Andes and identifies target areas for conservation. Biome distribution models were developed using logistic regressions. These models were then coupled to an ensemble of 8 global climate models to project future distribution of the Andean biomes and their uncertainties. We analysed projected changes in extent and elevational range and identified regions most prone to change. Our results show a heterogeneous response to climate change. Although the wetter biomes exhibit an upslope displacement of both the upper and the lower boundaries as expected, most dry biomes tend to show downslope expansion. Despite important losses being projected for several biomes, projections suggest that between 74.8% and 83.1% of the current total Tropical Andes will remain stable, depending on the emission scenario and time horizon. Between 3.3% and 7.6% of the study area is projected to change, mostly towards an increase in vertical structure. For the remaining area (13.1%-17.4%, there is no agreement between model projections. These results challenge the common believe that climate change will lead to an upslope displacement of biome boundaries in mountain regions. Instead, our models project diverging responses, including downslope expansion and large areas projected to remain stable. Lastly, a significant part of the area expected to change is already affected by land use changes, which has important implications for management. This, and the inclusion of a comprehensive uncertainty analysis, will help to inform conservation strategies in the Tropical Andes, and to guide similar

  15. Impact of cash cropping and perennial crops on food crop ...

    African Journals Online (AJOL)

    significant effects on food crop production and productivity. ... 2 Department of Economics and Resource management, Norwegian University of Life Sciences, Norway ... food markets work well, the problem of imperfect markets does not allow ..... prices at the time of purchase with the remaining balance due at the end of the.

  16. Morphological, thermal and physicochemical characteristics of small granules starch from Mirabilis jalapa L

    Energy Technology Data Exchange (ETDEWEB)

    Pumacahua-Ramos, Augusto [Department of Food Engineering, Universidad Peruana Unión, Juliaca (Peru); Paulista State University, IBILCE/UNESP, São Jose do Rio Preto, SP (Brazil); State University of Ponta Grossa–UEPG, Av. Carlos Cavalcanti, 4748, ZIP 84030-900 Ponta Grossa, PR (Brazil); Demiate, Ivo Mottin [State University of Ponta Grossa–UEPG, Av. Carlos Cavalcanti, 4748, ZIP 84030-900 Ponta Grossa, PR (Brazil); Schnitzler, Egon, E-mail: egons@uepg.br [State University of Ponta Grossa–UEPG, Av. Carlos Cavalcanti, 4748, ZIP 84030-900 Ponta Grossa, PR (Brazil); Bedin, Ana Cláudia [State University of Ponta Grossa–UEPG, Av. Carlos Cavalcanti, 4748, ZIP 84030-900 Ponta Grossa, PR (Brazil); Telis-Romero, Javier; Lopes-Filho, José Francisco [Paulista State University, IBILCE/UNESP, São Jose do Rio Preto, SP (Brazil)

    2015-02-20

    Highlights: • New source of small granules starch was studied. • DSC analysis allowed to determine the gelatinisation of starch from M. jalapa seeds. • Structural characteristics of granules were analysed by XRD and MEV techniques. • The rheological and thermal behaviour were analysed by RVA and TG–DTA. - Abstract: Some physicochemical and thermal properties of starch from Mirabilis jalapa L, seeds were evaluated. The starch was extracted after hulling and grinding the seeds and the flour obtained was suspended in 0.1% (m/v) NaOH solution for 12 h at 30 °C; it was then centrifuged, re-suspended, washed with deionised water and dried in an oven with circulating air at 40 °C for 12 h. The micro-images of starch granules were performed by using scanning electron (SEM) and non-contact atomic force microscopy (NC-AFM) techniques; X-ray diffraction and mid-infrared spectroscopy were both used to evaluate the relative crystallinity of the starch granules. Thermal analyses TG/DTG and DSC, were applied for the analysis of thermal behaviour of this starch and the cooking behaviour of its aqueous solution was studied by using a viscometer RVA. Thermogravimetry showed that once dehydrated, the starch was stable up to 292 °C after which two steps of decomposition occurred, which were attributed to decomposition and oxidation of organic matter, respectively. The gelatinisation temperature and enthalpy, as assessed by DSC analysis, were 82.1 °C and 5.67 J g{sup −1}, respectively. RVA analysis showed pasting temperature of 76.4 °C, with a low viscosity peak at 95 °C, low breakdown, and high tendency to retrograde during cooling. Microscopic results reveal that the starch granules had a spherical shape and 67.4% of them presented diameters smaller than 890 nm. The X-ray diffractogram showed a typical A-type pattern and a relative crystallinity of 34% with a FTIR of 1047/1022 cm{sup −1} and a ratio of 1.38.

  17. Preventive and therapeutic administration of an indigenous Lactobacillus sp. strain against Proteus mirabilis ascending urinary tract infection in a mouse model.

    Science.gov (United States)

    Fraga, Martín; Scavone, Paola; Zunino, Pablo

    2005-07-01

    Probiotics are increasingly being considered as non-pharmaceutical and safe potential alternatives for the treatment and prevention of a variety of pathologies including urinary tract infections. These are the most common infections in medical practice and are frequently treated with antibiotics, which have generated an intense selective pressure over bacterial populations. Proteus mirabilis is a common cause of urinary tract infections in catheterised patients and people with abnormalities of the urinary tract. In this work we isolated, identified and characterised an indigenous Lactobacillus murinus strain (LbO2) from the vaginal tract of a female mouse. In vitro characterisation of LbO2 included acid and bile salts tolerance, growth in urine, adherence to uroepithelial cells and in vitro antimicrobial activity. The selected strain showed interesting properties, suitable for its use as a probiotic. The ability of LbO2 to prevent and even treat ascending P. mirabilis urinary tract infection was assessed using an experimental model in the mouse. Kidney and bladder P. mirabilis counts were significantly lower in mice preventively treated with the probiotic than in non-treated mice. When LbO2 was used for therapeutic treatment, bladder counts of treated mice were significantly lower although no significant differences were detected in P. mirabilis kidney colonisation of treated and non-treated animals. These results are encouraging and prompt further research related to probiotic strains and the basis of their effects for their use in human and animal health.

  18. Detection of human antibodies binding with smooth and rough LPSs from Proteus mirabilis O3 strains S1959, R110, R45.

    Science.gov (United States)

    Gleńska-Olender, J; Durlik, K; Konieczna, I; Kowalska, P; Gawęda, J; Kaca, W

    2017-11-01

    Bacteria of the genus Proteus of the family Enterobacteriaceae are facultative human pathogens responsible mainly for urinary tract and wound infections, bacteremia and the development of rheumatoid arthritis (RA). We have analyzed and compared by ELISA the titer of antibodies in plasmas of healthy individuals and in sera of rheumatoid arthritis patients recognizing a potential host cross-reactive epitope (lysine-galacturonic acid epitopes) present in Proteus lipopolysaccharide (LPS). In our experiments LPSs isolated from two mutants of smooth Proteus mirabilis 1959 (O3), i.e. strains R110 and R45, were used. R110 (Ra type mutant) is lacking the O-specific polysaccharide, but possesses a complete core oligosaccharide, while R45 (Re type) has a reduced core oligosaccharide and contains two 3-deoxy-D-manno-oct-2-ulosonic acid residues and one of 4-amino-4-deoxy-L-arabinopyranose residues. Titer of P. mirabilis S1959 LPS-specific-antibodies increased with the age of blood donors. RA and blood donors' sera contained antibodies against S and Ra and Re type of P. mirabilis O3 LPSs. Antibodies recognizing lysine-galacturonic acid epitopes of O3 LPS were detected by ELISA in some plasmas of healthy individuals and sera of rheumatoid arthritis patients. RA patients antibodies reacting with P. mirabilis S1959 S and R LPSs may indicate a potential role of anti-LPS antibodies in molecular mimicry in RA diseases.

  19. Determination of a novel integron-located variant (blaOXA -320 ) of Class D β-lactamase in Proteus mirabilis.

    Science.gov (United States)

    Cicek, Aysegul Copur; Duzgun, Azer Ozad; Saral, Aysegul; Sandalli, Cemal

    2014-10-01

    Proteus mirabilis (P. mirabilis) is one of Gram-negative pathogens encountered in clinical specimens. A clinical isolate (TRP41) of P. mirabilis was isolated from a Turkish patient in Turkey. The isolate was identified using the API 32GN system and 16S rRNA gene sequencing and it was found resistant to ampicillin/sulbactam, piperacillin, tetracycline, and trimethoprim/sulfamethoxazole. This isolate was harboring a Class 1 integron gene cassette and its DNA sequence analysis revealed a novel blaOXA variant exhibiting one amino acid substitution (Asn266Ile) from blaOXA-1 . This new variant of OXA was located on Class 1 integron together with aadA1 gene encoding aminoglycoside-modifying enzymes. According to sequence records, the new variant was named as blaOXA-320 . Cassette array and size of integron were found as blaOXA-320 -aadA1 and 2086 bp, respectively. The blaOXA-320 gene is not transferable according to conjugation experiment. In this study, we report the first identification of blaOXA-320 -aadA1 gene cassette, a novel variant of Class D β-lactamase, in P. mirabilis from Turkey. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  20. Efeito da aplicação de maravilha (Mirabilis jalapa L., primavera (Bougainvillea spectabilis L. e isolados de Trichoderma na produção de alface Effect of four o'clock flowers (Mirabilis jalapa L., bougainvillea (Bougainvillea spectabilis L. and Trichoderma spp. isolates in production of lettuce

    Directory of Open Access Journals (Sweden)

    JA Azevedo Filho

    2011-01-01

    Full Text Available Objetivou-se com esse trabalho avaliar o efeito de extratos foliares de primavera (Bougainvillea spetabilis L. e maravilha (Mirabilis jalapa L. e de Trichoderma spp., isoladamente ou em combinação, no manejo de viroses e na produção de alface. Os ensaios foram conduzidos em área de produção de hortaliças folhosas no município de Pinhalzinho/SP. O delineamento experimental foi de blocos casualizados, com dez tratamentos comparados com controle químico convencional. Foram observadas reduções de 18% e 32% na população de bactérias da rizosfera nas plantas de alface tratadas com o isolado IB18/22 e extrato de primavera + isolados de Trichoderma spp., respectivamente. Verificou-se que o extrato de maravilha estimulou o aumento do número de UFC/g da população de fungos. Entretanto, não houve diferença significativa na massa fresca da parte aérea e, apesar da pressão de inóculo e da alta densidade populacional de insetos vetores de vírus no local, não foi constatada a ocorrência de viroses, com exceção do espessamento de nervuras, que ocorreu em menor número em plantas tratadas, comparadas as do controle. Economicamente, houve 27,8% de lucratividade e margem bruta de 38,5%, indicando a viabilidade do uso de ferramentas de base ecológica no cultivo de alface. Além disso, os produtos usados são de fácil aquisição, seguros em termos de aplicação, meio ambiente e para o consumidor.The aim of the work was to evaluate the effect of leaf extracts of bougainvillea (Bougainvillea spetabilis L. and of four o'clock flowers (Mirabilis jalapa L. plants and Trichoderma spp., alone or in combination, on viruses management and lettuce production. The tests were conducted in a producing area in Pinhalzinho-SP. The experimental design was randomized blocks with ten treatments in comparison with conventional chemical control. Reductions of 18% and 32% in the bacterial population of the lettuce rhizosphere treated with IB18/22 isolate

  1. Gender in crop agriculture

    OpenAIRE

    Food and Agriculture Organization; The World Bank; IFAD

    2008-01-01

    Metadata only record This is a module in the "Gender in Agriculture Sourcebook" published by the World Bank, UN Food and Agriculture Organization, and International Fund for Agricultural Development. This module examines the role of gender in crop agriculture as an essential component of development and poverty reduction. Gender is an integral aspect of crop agriculture because women's roles in crop production and household subsistence, as well as their knowledge of complex production syst...

  2. The spread of bla OXA-48 and bla OXA-244 carbapenemase genes among Klebsiella pneumoniae, Proteus mirabilis and Enterobacter spp. isolated in Moscow, Russia.

    Science.gov (United States)

    Fursova, Nadezhda K; Astashkin, Eugeny I; Knyazeva, Anastasia I; Kartsev, Nikolay N; Leonova, Ekaterina S; Ershova, Olga N; Alexandrova, Irina A; Kurdyumova, Natalia V; Sazikina, Svetlana Yu; Volozhantsev, Nikolay V; Svetoch, Edward A; Dyatlov, Ivan A

    2015-11-02

    The spread of carbapenemase-producing Enterobacteriaceae (CPE) is a great problem of healthcare worldwide. Study of the spread for bla OXA-48-like genes coding epidemically significant carbapenemases among hospital pathogens is important for the regional and global epidemiology of antimicrobial resistance. Antibacterial resistant isolates of Klebsiella pneumoniae (n = 95) from 54 patients, P. mirabilis (n = 32) from 20 patients, Enterobacter aerogenes (n = 6) from four patients, and Enterobacter cloacae (n = 4) from four patients were collected from January, 2013 to October, 2014 in neurosurgical intensive care unit (ICU) of the Burdenko Neurosurgery Institute, Moscow. Characteristics of the isolates were done using susceptibility tests, PCR detection of the resistance genes, genotyping, conjugation, DNA sequencing, and bioinformatic analysis. Major strains under study were multi drug resistant (MDR), resistant to three or more functional classes of drugs simultaneously-98.9 % K. pneumoniae, 100 % P. mirabilis, one E. aerogenes isolate, and one E. cloacae isolate. Molecular-genetic mechanism of MDR in K. pneumoniae and P. mirabilis isolates were based on carrying of epidemic extended-spectrum beta-lactamase bla CTX-M-15 gene (87.2 and 90.6 % accordingly), carbapenemase bla OXA-48-like gene (55.3 and 23.3 % accordingly), and class 1 (54.8 and 31.3 % accordingly) and class 2 (90.6 % P. mirabilis) integrons. The bla OXA-48-like-positive K. pneumoniae were collected during whole two-year surveillance period, while P. mirabilis and Enterobacter spp. carrying bla OXA-48-like genes were detected only after four and 18 months after the research start, respectively. The bla OXA-48-like gene acquisition was shown for P. mirabilis isolates collected from five patients and for E. cloacae isolate collected from one patient during their stay in the ICU, presumably from bla OXA-48-like-positive K. pneumoniae. The source of the bla OXA-244 gene acquired by E

  3. Assessment of satellite rainfall products over the Andean plateau

    Science.gov (United States)

    Satgé, Frédéric; Bonnet, Marie-Paule; Gosset, Marielle; Molina, Jorge; Hernan Yuque Lima, Wilson; Pillco Zolá, Ramiro; Timouk, Franck; Garnier, Jérémie

    2016-01-01

    Nine satellite rainfall estimations (SREs) were evaluated for the first time over the South American Andean plateau watershed by comparison with rain gauge data acquired between 2005 and 2007. The comparisons were carried out at the annual, monthly and daily time steps. All SREs reproduce the salient pattern of the annual rain field, with a marked north-south gradient and a lighter east-west gradient. However, the intensity of the gradient differs among SREs: it is well marked in the Tropical Rainfall Measuring Mission (TRMM) Multisatellite Precipitation Analysis 3B42 (TMPA-3B42), Precipitation Estimation from remotely Sensed Information using Artificial Neural Networks (PERSIANN) and Global Satellite Mapping of Precipitation (GSMaP) products, and it is smoothed out in the Climate prediction center MORPHing (CMORPH) products. Another interesting difference among products is the contrast in rainfall amounts between the water surfaces (Lake Titicaca) and the surrounding land. Some products (TMPA-3B42, PERSIANN and GSMaP) show a contradictory rainfall deficit over Lake Titicaca, which may be due to the emissivity contrast between the lake and the surrounding lands and warm rain cloud processes. An analysis differentiating coastal Lake Titicaca from inland pixels confirmed this trend. The raw or Real Time (RT) products have strong biases over the study region. These biases are strongly positive for PERSIANN (above 90%), moderately positive for TMPA-3B42 (28%), strongly negative for CMORPH (- 42%) and moderately negative for GSMaP (- 18%). The biases are associated with a deformation of the rain rate frequency distribution: GSMaP underestimates the proportion of rainfall events for all rain rates; CMORPH overestimates the proportion of rain rates below 2 mm day- 1; and the other products tend to overestimate the proportion of moderate to high rain rates. These biases are greatly reduced by the gauge adjustment in the TMPA-3B42, PERSIANN and CMORPH products, whereas a

  4. Characterization of Two Microbial Isolates from Andean Lakes in Bolivia

    Science.gov (United States)

    Demergasso, C.; Blamey, J.; Escudero, L.; Chong, G.; Casamayor, E. O.; Cabrol, N. A.; Grin, E. A.; Hock, A.; Kiss, A.; Borics, G.

    2004-01-01

    We are currently investigating the biological population present in the highest and least explored perennial lakes on earth in the Bolivian and Chilean Andes, including several volcanic crater lakes of more than 6000 m elevation, in combination of microbiological and molecular biological methods. Our samples were collected in saline lakes of the Laguna Blanca Laguna Verde area in the Bolivian Altiplano and in the Licancabur volcano crater (27 deg. 47 min S/67 deg. 47 min. W) in the ongoing project studying high altitude lakes. The main goal of the project is to look for analogies with Martian paleolakes. These Bolivian lakes can be described as Andean lakes following the classification of Chong. We have attempted to isolate pure cultures and phylogenetically characterize prokaryotes that grew under laboratory conditions. Sediment samples taken from the Licancabur crater lake (LC), Laguna Verde (LV), and Laguna Blanca (LB) were analyzed and cultured using enriched liquid media under both aerobic and anaerobic conditions. All cultures were incubated at room temperature (15 to 20 C) and under light exposure. For the reported isolates, 36 hours incubation were necessary for reaching optimal optical densities to consider them viable cultures. Ten serial dilutions starting from 1% inoculum were required to obtain a suitable enriched cell culture to transfer into solid media. Cultures on solid medium were necessary to verify the formation of colonies in order to isolate pure cultures. Different solid media were prepared using several combinations of both trace minerals and carbohydrates sources in order to fit their nutrient requirements. The microorganisms formed individual colonies on solid media enriched with tryptone, yeast extract and sodium chloride. Cells morphology was studied by optical and electronic microscopy. Rodshape morphologies were observed in most cases. Total bacterial genomic DNA was isolated from 50 ml late-exponential phase culture by using the CTAB

  5. Phytoregionalisation of the Andean páramo

    Directory of Open Access Journals (Sweden)

    Gwendolyn Peyre

    2018-06-01

    Full Text Available Background The páramo is a high-elevation biogeographical province in the northern Andes, known for its great biodiversity and ecosystem services. Because there have been very few biogeographic studies encompassing the entire province to date, this study aimed at conducting a phytogeographical regionalisation of the páramo. Specifically, (1 clustering analyses were conducted to identify the main phytogeographical units in the three altitudinal belts: sub-páramo, mid-páramo and super-páramo, and examine their diagnostic flora, (2 an ordination complemented the geo-climatic characterization of the obtained units and (3 a hierarchical classification transformation was obtained to evaluate the relationships between units. Methods The study area included the entire Andean páramo range in northern Peru, Ecuador, Colombia and Venezuela. The analyses were based on 1,647 phytosociological plots from the VegPáramo database. The K-means non-hierarchical clustering technique was used to obtain clusters identifiable as phytogeographical units, and the Ochiai fidelity index was calculated to identify their diagnostic species. A principal component analysis was conducted to obtain the geo-climatic characterization of each unit. Finally, the relationships between clusters were traced using a hierarchical plot-based classification. Results Fifteen clusters were obtained, 13 natural and two artificial, of which two represented the sub-páramo, nine the mid-páramo and four the super-páramo. Even though data representativeness was a potential limitation to segregate certain sub-páramo and super-páramo units, the overall bioregionalisation was robust and represented important latitudinal, altitudinal and climatic gradients. Discussion This study is the first to bioregionalise the páramo province based on a substantial widely distributed biological dataset, and therefore provides important novel scientific insight on its biogeography. The obtained

  6. Impact of potato cultivation and cattle farming on physicochemical parameters and enzymatic activities of Neotropical high Andean Páramo ecosystem soils.

    Science.gov (United States)

    Avellaneda-Torres, Lizeth Manuela; León Sicard, Tomás Enrique; Torres Rojas, Esperanza

    2018-08-01

    The Andean Páramos are high mountain ecosystems whose soils are essential for the management of South American water resources, but research on anthropic impacts to these soils is currently minimal and insufficient. The objective of this study was to evaluate the impacts of potato (Solanum tuberosum) cultivation and livestock on the physicochemical parameters and enzymatic activities that determine the soil quality of the Neotropical high Andean Páramo ecosystem in the Nevados National Natural Park (Nevados NNP) in Colombia. It was hypothesised that sites with potato crops and livestock farming would exhibit significant changes in soil physicochemical parameters and enzymatic activities compared with Páramo sites that have been conserved without agriculture. Samples were collected from soils under potato cultivation, livestock and Páramo (subject to the lowest degree of human intervention possible), on three farms in the El Bosque District at three different altitudes (Buenos Aires, El Edén and La Secreta) during two seasons (dry and rainy). The results showed that none of the physical parameters under study presented statistically significant differences due to the type of use (livestock, potato crop or Páramo), season of sampling (dry or rainy season) or altitude (different farms). The chemical parameters that statistically significantly differed due to land use were organic carbon, cation exchange capacity, calcium, potassium, and ammonium and those that showed statistically significant differences associated with the sampling timing were organic carbon, nitrogen, cation exchange capacity, total carbon, C/N and nitrate. Additionally, there were differences in organic carbon due to the altitude of the farms. With respect to enzymatic activities, those of β-glucosidase, phosphodiesterase and urease significantly decreased in soils under potato cultivation and livestock relative to those of Páramo, but those of acid phosphatase and protease increased

  7. Phylogeny and historical biogeography of trans-Andean cichlid fishes (Teleostei: Cichlidae)

    Czech Academy of Sciences Publication Activity Database

    Musilová, Zuzana; Říčan, O.; Říčanová, Š.; Janšta, P.; Gahura, O.; Novák, J.

    2015-01-01

    Roč. 65, č. 3 (2015), s. 333-350 ISSN 1864-5755 R&D Projects: GA MŠk LC06073 Institutional support: RVO:67985904 Keywords : Andean uplift * Andinoacara * Mesoheros Subject RIV: EG - Zoology Impact factor: 0.722, year: 2015

  8. Successional position of dry Andean dwarf forest species as a basis for restoration trials

    NARCIS (Netherlands)

    Groenendijk, J.P.; Duivenvoorden, J.F.; Cleef, A.M.; Rietman, N.

    2005-01-01

    The successional affinity of nine woody species was inferred from the structure, diversity and disturbance history of the vegetation where these occurred. This was done in order to obtain a basis for a restoration experiment, currently in execution, in the dry Andean dwarf forest zone on the edge of

  9. Dispersal, isolation and diversification with continued gene flow in an Andean tropical dry forest.

    Science.gov (United States)

    Toby Pennington, R; Lavin, Matt

    2017-07-01

    The Andes are the world's longest mountain chain, and the tropical Andes are the world's richest biodiversity hot spot. The origin of the tropical Andean cordillera is relatively recent because the elevation of the mountains was relatively low (400-2500 m palaeoelevations) only 10 MYA with final uplift being rapid. These final phases of the Andean orogeny are thought to have had a fundamental role in shaping processes of biotic diversification and biogeography, with these effects reaching far from the mountains themselves by changing the course of rivers and deposition of mineral-rich Andean sediments across the massive Amazon basin. In a recent issue of Molecular Ecology, Oswald, Overcast, Mauck, Andersen, and Smith (2017) investigate the biogeography and diversification of bird species in the Andes of Peru and Ecuador. Their study is novel in its focus on tropical dry forests (Figure 1) rather than more mesic biomes such as rain forests, cloud forests and paramos, which tend to be the focus of science and conservation in the Andean hot spot. It is also able to draw powerful conclusions via the first deployment of genomic approaches to a biogeographic question in the threatened dry forests of the New World. © 2017 John Wiley & Sons Ltd.

  10. Regional water resources management in the Andean region with numerical models and satellite remote sensing

    NARCIS (Netherlands)

    Menenti, M.; Mulders, C.W.B.

    1999-01-01

    This report describes the development and adaptation of distributed numerical simulation models of hydrological processes in complex watersheds typical of the Andean region. These distributed models take advantage of the synoptic capabilities of sensors on-board satellites and GIS procedures have

  11. 78 FR 15031 - Agency Information Collection Activities: Andean Trade Preferences Act

    Science.gov (United States)

    2013-03-08

    ... Management and Budget (OMB) approval. All comments will become a matter of public record. In this document..., Andean Trade Promotion and Drug Eradication Act (ATPDEA) Certificate of Origin. This form can be used... 17. Type of Review: Extension (without change). Affected Public: Businesses. ATPA Certificate of...

  12. Trade in Andean Condor Vulture gryphus feathers and body parts in ...

    African Journals Online (AJOL)

    body parts in the city of Cusco and the Sacred Valley,. Cusco region, Peru. Robert S. R. ... The sale of Andean Condor feathers and body parts is undertaken openly in the tourist markets of Cusco and the Sacred .... and shops. Prices in local currency – Nuevo Sol and US Dollar equivalent given in parentheses). Feather.

  13. The Rise and Fall of Andean Empires: El Nino History Lessons.

    Science.gov (United States)

    Wright, Kenneth R.

    2000-01-01

    Provides information on El Nino and the methods for investigating ancient climate record. Traces the rise and fall of the Andean empires focusing on the climatic forces that each empire (Tiwanaku, Wari, Moche, and Inca) endured. States that modern societies should learn from the experiences of these ancient civilizations. (CMK)

  14. Contrasting patterns of Andean diversification among three diverse clades of Neotropical clearwing butterflies.

    Science.gov (United States)

    Chazot, Nicolas; De-Silva, Donna Lisa; Willmott, Keith R; Freitas, André V L; Lamas, Gerardo; Mallet, James; Giraldo, Carlos E; Uribe, Sandra; Elias, Marianne

    2018-04-01

    The Neotropical region is the most biodiverse on Earth, in a large part due to the highly diverse tropical Andean biota. The Andes are a potentially important driver of diversification within the mountains and for neighboring regions. We compared the role of the Andes in diversification among three subtribes of Ithomiini butterflies endemic to the Neotropics, Dircennina, Oleriina, and Godyridina. The diversification patterns of Godyridina have been studied previously. Here, we generate the first time-calibrated phylogeny for the largest ithomiine subtribe, Dircennina, and we reanalyze a published phylogeny of Oleriina to test different biogeographic scenarios involving the Andes within an identical framework. We found common diversification patterns across the three subtribes, as well as major differences. In Dircennina and Oleriina, our results reveal a congruent pattern of diversification related to the Andes with an Andean origin, which contrasts with the Amazonian origin and multiple Andean colonizations of Godyridina. In each of the three subtribes, a clade diversified in the Northern Andes at a faster rate. Diversification within Amazonia occurred in Oleriina and Godyridina, while virtually no speciation occurred in Dircennina in this region. Dircennina was therefore characterized by higher diversification rates within the Andes compared to non-Andean regions, while in Oleriina and Godyridina, we found no difference between these regions. Our results and discussion highlight the importance of comparative approaches in biogeographic studies.

  15. Studies on Colombian Cryptogams VI. High Andean species of Radula (Hepaticae)

    NARCIS (Netherlands)

    Jans, Els

    1979-01-01

    This study deals with the genus Radula (Hepaticae) from the high Andean forests and paramos of Colombia, above 2500 m, and is based on the determination of Colombian collections gathered by H. Bischler and by A. M. Cleef and collaborators. A key to the 8 species known from the area is given and

  16. Genome-wide association study of anthracnose resistance in Andean beans

    Science.gov (United States)

    Anthracnose is a seed-borne disease of common bean (Phaseolus vulgaris L.) caused by the fungus Colletotrichum lindemuthianum, and the pathogen is cosmopolitan in distribution. The objectives of this study were to identify new sources of anthracnose resistance in a diverse panel of 230 Andean beans ...

  17. Marcos Zapata's "Last Supper": a feast of European religion and Andean culture.

    Science.gov (United States)

    Zendt, Christina

    2010-01-01

    In Marcos Zapata's 1753 painting of the Last Supper in Cuzco, Peru, Christian symbolism is filtered through Andean cultural tradition. Zapata was a late member of the Cuzco School of Painting, a group comprised of few European immigrants and handfuls of mestizo and Indian artists. The painters in Cuzco learned mostly from prints of European paintings, and their style tends to blend local culture into the traditional painting of their conquistadors. Imagery was the most successful tool used by the Spaniards in their quest to Christianize the Andean population. By teaching locals to paint Christian subjects, they were able to infuse Christianity into Andean traditions. Zapata's rendering of the Last Supper utilizes this cultural blending while staying true to the Christian symbolism within the subject. Instead of the traditional lamb, Zapata's Last Supper features a platter of cuy, or guinea pig, an Andean delicacy stocked with protein as well as cultural significance. Cuy was traditionally a sacrificial animal at Inca agricultural festivals and in this way it offers poignant parallel to the lamb, as a traditional Christian sacrificial animal.

  18. Forests of the tropical eastern Andean flank during the middle Pleistocene

    NARCIS (Netherlands)

    Cárdenas, M.L.; Gosling, W.D.; Pennington, R.T.; Poole, I.; Sherlock, S.C.; Mothes, P.

    2014-01-01

    Inter-bedded volcanic and organic sediments from Erazo (Ecuador) indicate the presence of four different forest assemblages on the eastern Andean flank during the middle Pleistocene. Radiometric dates (40Ar-39Ar) obtained from the volcanic ash indicate that deposition occurred between 620,000 and

  19. Numerical simulation of cropping

    DEFF Research Database (Denmark)

    Tvergaard, Viggo; Hutchinson, John W.

    2014-01-01

    Cropping is a cutting process whereby opposing aligned blades create a shearing failure by exerting opposing forces normal to the surfaces of a metal sheet or plate. Building on recent efforts to quantify cropping, this paper formulates a plane strain elastic-plastic model of a plate subject to s...

  20. Applied Crop Protection 2017

    DEFF Research Database (Denmark)

    Jørgensen, Lise Nistrup; Nielsen, Bent Jørgen; Mathiassen, Solvejg Kopp

    Linket til højre henviser til rapporten i trykt format til download. This publication contains results from crop protection trials which were carried out at the Department of Agroecology within the area of gricultural crops. Most of the results come from field trials, but results from greenhouse...

  1. Applied Crop Protection 2017

    DEFF Research Database (Denmark)

    Jørgensen, Lise Nistrup; Nielsen, Bent Jørgen; Mathiassen, Solvejg Kopp

    Linket til højre henviser til rapporten i trykt format til download. This publication contains results from crop protection trials which were carried out at the Department of Agroecology within the area of gricultural crops. Most of the results come from field trials, but results from greenhouse ...

  2. Applied crop protection 2016

    DEFF Research Database (Denmark)

    Jørgensen, Lise Nistrup; Nielsen, Bent Jørgen; Jensen, Peter Kryger

    This publication contains results from crop protection trials which were carried out at the Department of Agroecology within the area of agricultural crops. Most of the results come from field trials, but results from greenhouse and semi-field trials are also included. The report contains results...

  3. Identifying signatures of natural selection in Tibetan and Andean populations using dense genome scan data.

    Directory of Open Access Journals (Sweden)

    Abigail Bigham

    2010-09-01

    Full Text Available High-altitude hypoxia (reduced inspired oxygen tension due to decreased barometric pressure exerts severe physiological stress on the human body. Two high-altitude regions where humans have lived for millennia are the Andean Altiplano and the Tibetan Plateau. Populations living in these regions exhibit unique circulatory, respiratory, and hematological adaptations to life at high altitude. Although these responses have been well characterized physiologically, their underlying genetic basis remains unknown. We performed a genome scan to identify genes showing evidence of adaptation to hypoxia. We looked across each chromosome to identify genomic regions with previously unknown function with respect to altitude phenotypes. In addition, groups of genes functioning in oxygen metabolism and sensing were examined to test the hypothesis that particular pathways have been involved in genetic adaptation to altitude. Applying four population genetic statistics commonly used for detecting signatures of natural selection, we identified selection-nominated candidate genes and gene regions in these two populations (Andeans and Tibetans separately. The Tibetan and Andean patterns of genetic adaptation are largely distinct from one another, with both populations showing evidence of positive natural selection in different genes or gene regions. Interestingly, one gene previously known to be important in cellular oxygen sensing, EGLN1 (also known as PHD2, shows evidence of positive selection in both Tibetans and Andeans. However, the pattern of variation for this gene differs between the two populations. Our results indicate that several key HIF-regulatory and targeted genes are responsible for adaptation to high altitude in Andeans and Tibetans, and several different chromosomal regions are implicated in the putative response to selection. These data suggest a genetic role in high-altitude adaption and provide a basis for future genotype/phenotype association

  4. Andean Condor (Vultur gryphus) in Ecuador: Geographic Distribution, Population Size and Extinction Risk.

    Science.gov (United States)

    Naveda-Rodríguez, Adrián; Vargas, Félix Hernán; Kohn, Sebastián; Zapata-Ríos, Galo

    2016-01-01

    The Andean Condor (Vultur gryphus) in Ecuador is classified as Critically Endangered. Before 2015, standardized and systematic estimates of geographic distribution, population size and structure were not available for this species, hampering the assessment of its current status and hindering the design and implementation of effective conservation actions. In this study, we performed the first quantitative assessment of geographic distribution, population size and population viability of Andean Condor in Ecuador. We used a methodological approach that included an ecological niche model to study geographic distribution, a simultaneous survey of 70 roosting sites to estimate population size and a population viability analysis (PVA) for the next 100 years. Geographic distribution in the form of extent of occurrence was 49 725 km2. During a two-day census, 93 Andean Condors were recorded and a population of 94 to 102 individuals was estimated. In this population, adult-to-immature ratio was 1:0.5. In the modeled PVA scenarios, the probability of extinction, mean time to extinction and minimum population size varied from zero to 100%, 63 years and 193 individuals, respectively. Habitat loss is the greatest threat to the conservation of Andean Condor populations in Ecuador. Population size reduction in scenarios that included habitat loss began within the first 15 years of this threat. Population reinforcement had no effects on the recovery of Andean Condor populations given the current status of the species in Ecuador. The population size estimate presented in this study is the lower than those reported previously in other countries where the species occur. The inferences derived from the population viability analysis have implications for Condor management in Ecuador. This study highlights the need to redirect efforts from captive breeding and population reinforcement to habitat conservation.

  5. Design, synthesis, molecular docking, anti-Proteus mirabilis and urease inhibition of new fluoroquinolone carboxylic acid derivatives.

    Science.gov (United States)

    Abdullah, Mohammed A A; Abuo-Rahma, Gamal El-Din A A; Abdelhafez, El-Shimaa M N; Hassan, Heba A; Abd El-Baky, Rehab M

    2017-02-01

    New hydroxamic acid, hydrazide and amide derivatives of ciprofloxacin in addition to their analogues of levofloxacin were prepared and identified by different spectroscopic techniques. Some of the prepared compounds revealed good activity against the urease splitting bacteria, Proteus mirabilis. The urease inhibitory activity was investigated using indophenol method. Most of the tested compounds showed better activity than the reference acetohydroxamic acid (AHA). The ciprofloxacin hydrazide derivative 3a and levofloxacin hydroxamic acid 7 experienced the highest activity (IC 50 =1.22μM and 2.20μM, respectively). Molecular docking study revealed high spontaneous binding ability of the tested compounds to the active site of urease. Copyright © 2016 Elsevier Inc. All rights reserved.

  6. Proteus genomic island 1 (PGI1), a new resistance genomic island from two Proteus mirabilis French clinical isolates.

    Science.gov (United States)

    Siebor, Eliane; Neuwirth, Catherine

    2014-12-01

    To analyse the genetic environment of the antibiotic resistance genes in two clinical Proteus mirabilis isolates resistant to multiple antibiotics. PCR, gene walking and whole-genome sequencing were used to determine the sequence of the resistance regions, the surrounding genetic structure and the flanking chromosomal regions. A genomic island of 81.1 kb named Proteus genomic island 1 (PGI1) located at the 3'-end of trmE (formerly known as thdF) was characterized. The large MDR region of PGI1 (55.4 kb) included a class 1 integron (aadB and aadA2) and regions deriving from several transposons: Tn2 (blaTEM-135), Tn21, Tn6020-like transposon (aphA1b), a hybrid Tn502/Tn5053 transposon, Tn501, a hybrid Tn1696/Tn1721 transposon [tetA(A)] carrying a class 1 integron (aadA1) and Tn5393 (strA and strB). Several ISs were also present (IS4321, IS1R and IS26). The PGI1 backbone (25.7 kb) was identical to that identified in Salmonella Heidelberg SL476 and shared some identity with the Salmonella genomic island 1 (SGI1) backbone. An IS26-mediated recombination event caused the division of the MDR region into two parts separated by a large chromosomal DNA fragment of 197 kb, the right end of PGI1 and this chromosomal sequence being in inverse orientation. PGI1 is a new resistance genomic island from P. mirabilis belonging to the same island family as SGI1. The role of PGI1 in the spread of antimicrobial resistance genes among Enterobacteriaceae of medical importance needs to be evaluated. © The Author 2014. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

  7. Secondary metabolites produced by marine streptomyces as antibiofilm and quorum-sensing inhibitor of uropathogen Proteus mirabilis.

    Science.gov (United States)

    Younis, Khansa Mohammed; Usup, Gires; Ahmad, Asmat

    2016-03-01

    Quorum-sensing regulates bacterial biofilm formation and virulence factors, thereby making it an interesting target for attenuating pathogens. In this study, we investigated anti-biofilm and anti-quorum-sensing compounds from secondary metabolites of halophiles marine streptomyces against urinary catheter biofilm forming Proteus mirabilis without effect on growth viability. A total of 40 actinomycetes were isolated from samples collected from different places in Iraq including marine sediments and soil samples. Fifteen isolates identified as streptomyces and their supernatant screened as anti-quorum-sensing by inhibiting quorum-sensing regulated prodigiosin biosynthesis of Serratia marcescens strain Smj-11 as a reporter strain. Isolate Sediment Lake Iraq (sdLi) showed potential anti-quorum-sensing activity. Out of 35 clinical isolates obtained from Urinary catheter used by patient at the Universiti Kebangsaan Malaysia Medical Center, 22 isolates were characterized and identified as Proteus mirabilis. Isolate Urinary Catheter B4 (UCB4) showed the highest biofilm formation with highest resistance to used antibiotic and was chosen for further studies. Ethyl acetate secondary metabolites extract was produced from sdLi isolate. First, we determined the Minimum Inhibitory Concentration (MIC) of sdLi crude extract against UCB4 isolate, and all further experiments used concentrations below the MIC. Tests of subinhibitory concentrations of sdLi crude extract showed good inhibition against UCB4 isolate biofilm formation on urinary catheter and cover glass using Scanning electron microscopy and light microscopy respectively. The influence of sub-MIC of sdLi crude extract was also found to attenuate the quorum sensing (QS)-dependent factors such as hemolysin activity, urease activity, pH value, and motility of UCB4 isolate. Evidence is presented that these nontoxic secondary metabolites may act as antagonists of bacterial quorum sensing by competing with quorum-sensing signals

  8. Repair of single-strand breaks induced in the DNA of Proteus mirabilis by excision repair after UV-irradiation

    International Nuclear Information System (INIS)

    Stoerl, K.; Mund, C.

    1977-01-01

    Single-strand breaks have been produced in the DNA of P. mirabilis after UV-irradiation in dependence on the incident UV-doses. It has been found that there exists a discrepancy between the single-strand breaks estimated from sedimentation in alkaline sucrose gradients and the expected single-strand breaks approximated from measurements of dimer excision. The low number in incision breaks observed by sedimentation experiments is an indication that the cells are able to repair the excision-induced breaks as fast as they are formed. Toluenized cells have been used for investigation of the incision step independently of subsequent repair processes. In presence of NMN the appearance of more single-strand breaks in the DNA has been observed. Furthermore, the number of incision breaks in toluenized cells increased in presence of exogenous ATP. The completion of the excision repair process has been investigated by observing the rejoining of incision breaks. After irradiation with UV-doses higher than approximately 240 erg/mm 2 the number of single-strand breaks remaining unrepaired in the DNA increased. Studies of the influence of nutrition conditions on the repair process have shown approximately the same capacity for repair of single-strand breaks in growth medium as well as in buffer. Progress in the excision repair was also followed by investigation of the DNA synthesized at the template-DNA containing the pyrimidine dimers. In comparison with E. coli, P. mirabilis showed a somewhat lower efficiency for the repair of single-strand breaks during the excision repair. (author)

  9. [Influence of Mirabilis jalapa Linn. Growth on the Microbial Community and Petroleum Hydrocarbon Degradation in Petroleum Contaminated Saline-alkali Soil].

    Science.gov (United States)

    Jiao, Hai-hua; Cui, Bing-jian; Wu, Shang-hua; Bai, Zhi-hui; Huang, Zhan-bin

    2015-09-01

    In order to explore the effect of Mirabilis jalapa Linn. growth on the structure characteristics of the microbial community and the degradation of petroleum hydrocarbon (TPH) in the petroleum-contaminated saline-alkali soil, Microbial biomass and species in the rhizosphere soils of Mirabilis jalapa Linn. in the contaminated saline soil were studied with the technology of phospholipid fatty acids (PLFAs) analysis. The results showed that comparing to CK soils without Mirabilis jalapa Linn., the ratio of PLFAs species varied were 71. 4%, 69. 2% and 33. 3% in the spring, summer and autumn season, respectively. In addition, there was distinct difference of the biomasses of the microbial community between the CK and rhizosphere soils and among the difference seasons of growth of Mirabilis jalapa Linn.. Compare to CK soil, the degradation rates of total petroleum hydrocarbon (TPH) was increased by 47. 6%, 28. 3%, and 18. 9% in spring, summer, and autumn rhizosphere soils, respectively. Correlation analysis was used to determine the correlation between TPH degradation and the soil microbial community. 77. 8% of the total soil microbial PLFAs species showed positive correlation to the TPH degradation (the correlation coefficient r > 0), among which, 55. 6% of PLFAs species showed high positive correlation(the correlation coefficient was r≥0. 8). In addition, the relative content of SAT and MONO had high correlation with TPH degradation in the CK sample soils, the corelation coefficient were 0. 92 and 0. 60 respectively; However, the percent of positive correlation was 42. 1% in the rhizosphere soils with 21. 1% of them had high positive correlation. The relative content of TBSAT, MONO and CYCLO had moderate or low correlation in rhizosphere soils, and the correlation coefficient were 0. 56, 0. 50, and 0. 07 respectively. Our study showed that the growth of mirabilis Mirabilis jalapa Linn. had a higher influence on the species and biomass of microbial community in the

  10. Phenotypic, Molecular, and Pathological Characterization of Colletotrichum acutatum Associated with Andean Lupine and Tamarillo in the Ecuadorian Andes

    NARCIS (Netherlands)

    Falconi, C.; Visser, R.G.F.; Heusden, van A.W.

    2013-01-01

    Anthracnose is a serious problem of both Andean lupine and tamarillo in Ecuador. Morphological features, internal transcribed spacer (ITS) sequences, and host specificity were used to characterize Colletotrichum isolates from lupine and tamarillo. Based on phenotypic and molecular characterization,

  11. African Crop Science Journal

    African Journals Online (AJOL)

    AFRICAN JOURNALS ONLINE (AJOL) · Journals · Advanced Search · USING AJOL ... The African Crop Science Journal, a quarterly publication, publishes original ... interactions, information science, environmental science and soil science.

  12. African Crop Science Journal

    African Journals Online (AJOL)

    African Crop Science Journal. Journal Home · ABOUT THIS JOURNAL · Advanced Search · Current Issue · Archives · Journal Home > Vol 1, No 2 (1993) >. Log in or Register to get access to full text downloads.

  13. African Crop Science Journal

    African Journals Online (AJOL)

    African Crop Science Journal. Journal Home · ABOUT THIS JOURNAL · Advanced Search · Current Issue · Archives · Journal Home > Vol 22 (2014) >. Log in or Register to get access to full text downloads.

  14. Radioactivity in food crops

    International Nuclear Information System (INIS)

    Drury, J.S.; Baldauf, M.F.; Daniel, E.W.; Fore, C.S.; Uziel, M.S.

    1983-05-01

    Published levels of radioactivity in food crops from 21 countries and 4 island chains of Oceania are listed. The tabulation includes more than 3000 examples of 100 different crops. Data are arranged alphabetically by food crop and geographical origin. The sampling date, nuclide measured, mean radioactivity, range of radioactivities, sample basis, number of samples analyzed, and bibliographic citation are given for each entry, when available. Analyses were reported most frequently for 137 Cs, 40 K, 90 Sr, 226 Ra, 228 Ra, plutonium, uranium, total alpha, and total beta, but a few authors also reported data for 241 Am, 7 Be, 60 Co, 55 Fe, 3 H, 131 I, 54 Mn, 95 Nb, 210 Pb, 210 Po, 106 Ru, 125 Sb, 228 Th, 232 Th, and 95 Zr. Based on the reported data it appears that radioactivity from alpha emitters in food crops is usually low, on the order of 0.1 Bq.g -1 (wet weight) or less. Reported values of beta radiation in a given crop generally appear to be several orders of magnitude greater than those of alpha emitters. The most striking aspect of the data is the great range of radioactivity reported for a given nuclide in similar food crops with different geographical origins

  15. Radioactivity in food crops

    Energy Technology Data Exchange (ETDEWEB)

    Drury, J.S.; Baldauf, M.F.; Daniel, E.W.; Fore, C.S.; Uziel, M.S.

    1983-05-01

    Published levels of radioactivity in food crops from 21 countries and 4 island chains of Oceania are listed. The tabulation includes more than 3000 examples of 100 different crops. Data are arranged alphabetically by food crop and geographical origin. The sampling date, nuclide measured, mean radioactivity, range of radioactivities, sample basis, number of samples analyzed, and bibliographic citation are given for each entry, when available. Analyses were reported most frequently for /sup 137/Cs, /sup 40/K, /sup 90/Sr, /sup 226/Ra, /sup 228/Ra, plutonium, uranium, total alpha, and total beta, but a few authors also reported data for /sup 241/Am, /sup 7/Be, /sup 60/Co, /sup 55/Fe, /sup 3/H, /sup 131/I, /sup 54/Mn, /sup 95/Nb, /sup 210/Pb, /sup 210/Po, /sup 106/Ru, /sup 125/Sb, /sup 228/Th, /sup 232/Th, and /sup 95/Zr. Based on the reported data it appears that radioactivity from alpha emitters in food crops is usually low, on the order of 0.1 Bq.g/sup -1/ (wet weight) or less. Reported values of beta radiation in a given crop generally appear to be several orders of magnitude greater than those of alpha emitters. The most striking aspect of the data is the great range of radioactivity reported for a given nuclide in similar food crops with different geographical origins.

  16. Addressing crop interactions within cropping systems in LCA

    DEFF Research Database (Denmark)

    Goglio, Pietro; Brankatschk, Gerhard; Knudsen, Marie Trydeman

    2018-01-01

    objectives of this discussion article are as follows: (i) to discuss the characteristics of cropping systems which might affect the LCA methodology, (ii) to discuss the advantages and the disadvantages of the current available methods for the life-cycle assessment of cropping systems, and (iii) to offer...... management and emissions, and (3) functional unit issues. The LCA approaches presented are as follows: cropping system, allocation approaches, crop-by-crop approach, and combined approaches. The various approaches are described together with their advantages and disadvantages, applicability...... considers cropping system issues if they are related to multiproduct and nutrient cycling, while the crop-by-crop approach is highly affected by assumptions and considers cropping system issues only if they are related to the analyzed crop. Conclusions Each LCA approach presents advantages and disadvantages...

  17. Grand challenges for crop science

    Science.gov (United States)

    Crop science is a highly integrative science using the disciplines of conventional plant breeding, transgenic crop improvement, plant physiology, and cropping system sciences to develop improved varieties of agronomic, turf, and forage crops to produce feed, food, fuel, and fiber for our world's gro...

  18. Biotechnology: herbicide-resistant crops

    Science.gov (United States)

    Transgenic, herbicide-resistant (HR) crops are planted on about 80% of the land covered by transgenic crops. More than 90% of HR crios are glyphosate-resistant (GR) crops, the others being resistant to glufosinate. The wide-scale adoption of HR crops, largely for economic reasons, has been the mos...

  19. Active avoidance from a crude oil soluble fraction by an Andean paramo copepod.

    Science.gov (United States)

    Araújo, Cristiano V M; Moreira-Santos, Matilde; Sousa, José P; Ochoa-Herrera, Valeria; Encalada, Andrea C; Ribeiro, Rui

    2014-09-01

    Several oil spills due to ruptures in the pipeline oil systems have occurred at the Andean paramo. A sample of this crude oil was mixed with water from a nearby Andean lagoon and the toxicity of the soluble fraction was assessed through lethal and avoidance assays with a locally occurring copepod (Boeckella occidentalis intermedia). The integration of mortality and avoidance aimed at predicting the immediate decline of copepod populations facing an oil leakage. The 24-h median lethal PAH concentration was 42.7 (26.4-91.6) µg L(-1). In the 12-h avoidance assay, 30% avoidance was recorded at the highest PAH concentration (19.4 µg L(-1)). The mortality at this PAH concentration would be of 25% and, thus, the population immediate decline would be of 55%. The inclusion of non-forced exposure testing with the quantification of the avoidance response in environmental risk assessments is, therefore, supported due to underestimation of the lethal assays.

  20. Diversity and ecological ranges of plant species from dry inter-Andean valleys

    DEFF Research Database (Denmark)

    Quintana, Catalina

    found on steep slopes and in ravines. These areas of original dry valley vegetation preserve many wild relatives of cultivated plants on the one hand and old lineages of other wild plant groups. Dry inter-Andean valleys (DIAVs) in Ecuador therefore makeup a biodiversity hot spot for both plants......Dry valleys in the American Andes and other mountains have provided excellent agricultural lands since millennia. Besides agriculture, wood extraction and the establishment of urban areas have diminished the native vegetation of these valleys. Consequently the original vegetation is now mostly...... and animals, but unfortunately only very few botanical studies have been carried out in these areas. This thesis intends to shed light on the vegetation of the Dry Ecuadorean Inter-Andean Valleys in four chapters, each with a different focus. 1) A review paper that summarizes all scientific knowledge...

  1. Energy from field crops

    Energy Technology Data Exchange (ETDEWEB)

    Zubr, J.

    1990-04-15

    At the Research Station of Royal Veterinary and Agricultural University, Copenhagen, Denmark, investigation concerning cultivation and exploitation of field crops for production of fuels was carried out during the period 1986-1989. High yielding crops, such as sugar beet - BETA VULGARIS, jerusalem artichoke - HELIANTHUS TUBEROSUS, rhubarb - RHEUM RHAPONTICUM, and comfrey - SYMPHYTUM ASPERUM, were grown experimentally in the field. Different cultivation methods for the crops were used and evaluated. Simultaneously with the field experiment, laboratory investigation was carried out to determine the energy potential of different products and by-products from the crops processes, such as alcoholic and methanogenic fermantation. Production expenses for the crops were determined, and cost of the fuels was estimated. The experimental results show that beet is a superior crop for the climatic conditions of Northern Europe. In the season 1986, yields exceeded 20 t TS/ha in the form of roots and tops, where achieved. A combined exploitation of beet roots and tops via alcoholic and methanogenic fermantation gave a gross energy corresponding to 80 hl OE/ha/yr. Using methanogenic fermentation exclusively, from ensiled beet roots and tops, gross energy yield corresponding to 85 hl IE/ha/yr, was achieved. The cost of energy in the form of alcohol from beet roots was estimated to be 5.17 DKK/1 OE (0.64 ECU/l OE). The cost of energy in the form of methane from ensiled beet tops, was estimated to be 2.68 DKK/l OE (0.33 ECU/l OE). At the present time, methane produced on the basis of ensiled beet roots and tops appears to be competitive with fossil fuels. Irrespective of the cost, however, the possibility of producing clean energy from field crops remains of interest for the future. (author) 27 refs.

  2. Metabolic rates, enzyme activities and chemical compositions of some deep-sea pelagic worms, particularly Nectonemertes mirabilis (Nemertea; Hoplonemertinea) and Poeobius meseres (Annelida; Polychaeta)

    Science.gov (United States)

    Thuesen, Erik V.; Childress, James J.

    1993-05-01

    Investigations of metabolic rate, enzyme activity and chemical composition were undertaken on two abundant deep-sea pelagic worms: Nectonemertes mirabilis (Nemertea; Hoplonemertinea) and Poeobius meseres (Annelida; Polychaeta). Six other species of worms ( Pelagonemertes brinkmanni (Nemertea) and the following polychaetes: Pelagobia species A, Tomopteris nisseni, Tomopteris pacifica, Tomopteris species A, and Traviopsis lobifera) were captured in smaller numbers and used for comparison in the physiological and biochemical measurements. Polychaete worms had the highest oxygen consumption rates and, along with N. mirabilis, displayed significant size effects on metabolic rate. Poeobius meseres had the lowest rates of oxygen consumption and displayed no significant relationship of oxygen consumption rate to wet weight. No significant effect of size on the activities of citrate synthase, lactate dehydrogenase or pyruvate kinase was observed in P. meseres or N. mirabilis. Lipid content was higher than protein content for all the worms in this study. Carbohydrate was of little significance in these worms and was usually metabolic rates. It appears that polychaete worms as a group have higher metabolic rates than bathypelagic shrimps, copepods and fishes, and may be the animals with the highest metabolic rates in the bathypelagic regions of the world's oceans.

  3. DNA barcoding, ecology and geography of the cryptic species of Aneura pinguis and their relationships with Aneura maxima and Aneura mirabilis (Metzgeriales, Marchantiophyta).

    Science.gov (United States)

    Bączkiewicz, Alina; Szczecińska, Monika; Sawicki, Jakub; Stebel, Adam; Buczkowska, Katarzyna

    2017-01-01

    Aneura pinguis is a thalloid liverwort species with broad geographical distribution. It is composed of cryptic species, however, the number of cryptic species within A. pinguis is not known. Five cpDNA regions (matK, rbcL, rpoC1, trnH-psbA and trnL-trnF) and the entire nuclear ITS region were studied in 130 samples of A. pinguis from different geographical regions. The relationships between the cryptic species of A. pinguis, A. maxima and A. mirabilis were analyzed. All of the examined samples were clustered into 10 clades corresponding to 10 cryptic species of A. pinguis (marked A to J). Aneura mirabilis and A. maxima were nested among different cryptic species of A. pinguis, which indicates that A. pinguis is a paraphyletic taxon. Subgroups were found in cryptic species A, B, C and E. As single barcodes, all tested DNA regions had 100% discriminant power and fulfilled DNA barcode criteria for species identification; however, the only combination detected in all subgroups was trnL-trnF with trnH-psbA or ITS2. The distances between cryptic species were 11- to 35-fold higher than intraspecific distances. In all analyzed DNA regions, the distances between most pairs of cryptic A. pinguis species were higher than between A. maxima and A. mirabilis. All cryptic species of A. pinguis clearly differed in their habitat preferences, which suggests that habitat adaptation could be the main driving force behind cryptic speciation within this taxon.

  4. Comparative in vitro studies on disodium EDTA effect with and without Proteus mirabilis on the crystallization of carbonate apatite and struvite

    Science.gov (United States)

    Prywer, Jolanta; Olszynski, Marcin; Torzewska, Agnieszka; Mielniczek-Brzóska, Ewa

    2014-06-01

    Effect of disodium EDTA (salt of ethylenediamine tetraacetic acid) on the crystallization of struvite and carbonate apatite was studied. To evaluate such an effect we performed an experiment of struvite and carbonate apatite growth from artificial urine. The crystallization process was induced by Proteus mirabilis to mimic the real urinary tract infection, which usually leads to urinary stone formation. The results demonstrate that disodium EDTA exhibits the effect against P. mirabilis retarding the activity of urease - an enzyme produced by these microorganisms. The spectrophotometric results demonstrate that, with and without P. mirabilis, the addition of disodium EDTA increases the induction time and decreases the growth efficiency compared to the baseline (without disodium EDTA). These results are discussed from the standpoint of speciation of complexes formed in the solution of artificial urine in the presence of disodium EDTA. The size of struvite crystals was found to decrease in the presence of disodium EDTA. However, struvite crystals are larger in the presence of bacteria while the crystal morphology and habit remain unchanged.

  5. Anti-biofilm activity of biogenic selenium nanoparticles and selenium dioxide against clinical isolates of Staphylococcus aureus, Pseudomonas aeruginosa, and Proteus mirabilis.

    Science.gov (United States)

    Shakibaie, Mojtaba; Forootanfar, Hamid; Golkari, Yaser; Mohammadi-Khorsand, Tayebe; Shakibaie, Mohammad Reza

    2015-01-01

    The aim of the present study was to investigate the anti-biofilm activity of biologically synthesized selenium nanoparticles (Se NPs) against the biofilm produced by clinically isolated bacterial strains compared to that of selenium dioxide. Thirty strains of Staphylococcus aureus, Pseudomonas aeruginosa, and Proteus mirabilis were isolated from various specimens of the patients hospitalized in different hospitals (Kerman, Iran). Quantification of the biofilm using microtiter plate assay method introduced 30% of S. aureus, 13% of P. aeruginosa and 17% of P. mirabilis isolates as severely adherent strains. Transmission electron micrograph (TEM) of the purified Se NPs (produced by Bacillus sp. MSh-1) showed individual and spherical nano-structure in the size range of 80-220nm. Obtained results of the biofilm formation revealed that selenium nanoparticles inhibited the biofilm of S. aureus, P. aeruginosa, and P. mirabilis by 42%, 34.3%, and 53.4%, respectively, compared to that of the non-treated samples. Effect of temperature and pH on the biofilm formation in the presence of Se NPs and SeO2 was also evaluated. Copyright © 2014 Elsevier GmbH. All rights reserved.

  6. Proliferation of hydroelectric dams in the Andean Amazon and implications for Andes-Amazon connectivity.

    Science.gov (United States)

    Finer, Matt; Jenkins, Clinton N

    2012-01-01

    Due to rising energy demands and abundant untapped potential, hydropower projects are rapidly increasing in the Neotropics. This is especially true in the wet and rugged Andean Amazon, where regional governments are prioritizing new hydroelectric dams as the centerpiece of long-term energy plans. However, the current planning for hydropower lacks adequate regional and basin-scale assessment of potential ecological impacts. This lack of strategic planning is particularly problematic given the intimate link between the Andes and Amazonian flood plain, together one of the most species rich zones on Earth. We examined the potential ecological impacts, in terms of river connectivity and forest loss, of the planned proliferation of hydroelectric dams across all Andean tributaries of the Amazon River. Considering data on the full portfolios of existing and planned dams, along with data on roads and transmission line systems, we developed a new conceptual framework to estimate the relative impacts of all planned dams. There are plans for 151 new dams greater than 2 MW over the next 20 years, more than a 300% increase. These dams would include five of the six major Andean tributaries of the Amazon. Our ecological impact analysis classified 47% of the potential new dams as high impact and just 19% as low impact. Sixty percent of the dams would cause the first major break in connectivity between protected Andean headwaters and the lowland Amazon. More than 80% would drive deforestation due to new roads, transmission lines, or inundation. We conclude with a discussion of three major policy implications of these findings. 1) There is a critical need for further strategic regional and basin scale evaluation of dams. 2) There is an urgent need for a strategic plan to maintain Andes-Amazon connectivity. 3) Reconsideration of hydropower as a low-impact energy source in the Neotropics.

  7. MORPHOLOGICAL CONSTRAINTS AND NECTAR ROBBING IN THREE ANDEAN BUMBLE BEE SPECIES (HYMENOPTERA, APIDAE, BOMBINI

    Directory of Open Access Journals (Sweden)

    RIVEROS ANDRE J.

    2006-06-01

    Full Text Available We report differences in foraging behavior of three Andean bumblebee species onflowers of Digitalis purpurea (Scrophulariaceae. Bombus atratus was a potentialpollinator while B. hortulanus and B. rubicundus collected nectar by robbing throughholes. We attribute behavioral differences to physical constraints. B. atratus has alonger glossa and a larger body size and is able to reach the nectaries, whereas B.hortulanus and B. rubicundus have shorter glossae and smaller bodies and probablymust rob nectar through holes at the base of flowers.

  8. Maternal Knowledge and Use of Galactagogues in Andean Communities of Cusco, Peru

    Directory of Open Access Journals (Sweden)

    Madalena Monteban

    2017-09-01

    Full Text Available A commonly reported reason for early breastfeeding cessation is inadequate milk production. In response, women across the globe turn to galactagogues – substances used to increase the milk supply. Andean women have traditional knowledge about the medicinal and nutritional properties of plants and animals that are considered good to eat during breastfeeding. This research explores the maintenance and use of galactagogues, and specifically the use of the Andean flicker bird, within the wider framework of breastfeeding and nutrition policies in Peru. To elicit maternal knowledge and use of galactagogues, semi-structured and free-listing interviews were conducted with 33 mothers. Data analysis calculated the frequency and percentage of women reporting each type of galactagogue. In addition, thematic codes and relevant text passages were used in an iterative analytic process to document emerging themes. Identified galactagogues included five plants and six animals. Several galactagogues included protein-rich foods such as lamb meat and the Andean flicker bird. The use of protein-rich galactagogues as solid food is reinforced by public health messages. However, galactagogues in the research communities are usually consumed as soups or drinks, which are less rich in proteins than solid meals. The potential role of liquid galactagogues in the maintenance of appropriate hydration levels during breastfeeding in an environment where safe drinking water is scarce is a new contribution to the existing literature. The results are relevant to the design of maternal and child health policies that comply with intercultural health premises that value and respect the knowledge and practices of Andean Peoples.

  9. Coca: The History and Medical Significance of an Ancient Andean Tradition

    Directory of Open Access Journals (Sweden)

    Amy Sue Biondich

    2016-01-01

    Full Text Available Coca leaf products are an integral part of the lives of the Andean peoples from both a cultural and traditional medicine perspective. Coca is also the whole plant from which cocaine is derived. Coca products are thought to be a panacea for health troubles in regions of South America. This review will examine the toxicology of whole coca and will also look at medicinal applications of this plant, past, present, and future.

  10. Proliferation of hydroelectric dams in the Andean Amazon and implications for Andes-Amazon connectivity.

    Directory of Open Access Journals (Sweden)

    Matt Finer

    Full Text Available Due to rising energy demands and abundant untapped potential, hydropower projects are rapidly increasing in the Neotropics. This is especially true in the wet and rugged Andean Amazon, where regional governments are prioritizing new hydroelectric dams as the centerpiece of long-term energy plans. However, the current planning for hydropower lacks adequate regional and basin-scale assessment of potential ecological impacts. This lack of strategic planning is particularly problematic given the intimate link between the Andes and Amazonian flood plain, together one of the most species rich zones on Earth. We examined the potential ecological impacts, in terms of river connectivity and forest loss, of the planned proliferation of hydroelectric dams across all Andean tributaries of the Amazon River. Considering data on the full portfolios of existing and planned dams, along with data on roads and transmission line systems, we developed a new conceptual framework to estimate the relative impacts of all planned dams. There are plans for 151 new dams greater than 2 MW over the next 20 years, more than a 300% increase. These dams would include five of the six major Andean tributaries of the Amazon. Our ecological impact analysis classified 47% of the potential new dams as high impact and just 19% as low impact. Sixty percent of the dams would cause the first major break in connectivity between protected Andean headwaters and the lowland Amazon. More than 80% would drive deforestation due to new roads, transmission lines, or inundation. We conclude with a discussion of three major policy implications of these findings. 1 There is a critical need for further strategic regional and basin scale evaluation of dams. 2 There is an urgent need for a strategic plan to maintain Andes-Amazon connectivity. 3 Reconsideration of hydropower as a low-impact energy source in the Neotropics.

  11. Morphological and molecular diversity in a collection of the Andean tree tomato (Solanum betaceum Cav.)

    OpenAIRE

    Acosta-Quezada, P.G.; Martinez-Laborde, J.B.; Vilanova Navarro, Santiago; Prohens Tomás, Jaime

    2011-01-01

    Instituto Nacional de Investigaciones Agrarias y Agroalimentarias (RF2008-00008-00-00) Acosta-Quezada, P.; Martinez-Laborde, J.; Vilanova Navarro, S.; Prohens Tomás, J. (2011). Morphological and molecular diversity in a collection of the Andean tree tomato (Solanum betaceum Cav.). Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca : Horticulture. 68(1):500-501. http://hdl.handle.net/10251/63083 Senia 500 501 68 1

  12. Natural Selection on Genes Related to Cardiovascular Health in High-Altitude Adapted Andeans.

    Science.gov (United States)

    Crawford, Jacob E; Amaru, Ricardo; Song, Jihyun; Julian, Colleen G; Racimo, Fernando; Cheng, Jade Yu; Guo, Xiuqing; Yao, Jie; Ambale-Venkatesh, Bharath; Lima, João A; Rotter, Jerome I; Stehlik, Josef; Moore, Lorna G; Prchal, Josef T; Nielsen, Rasmus

    2017-11-02

    The increase in red blood cell mass (polycythemia) due to the reduced oxygen availability (hypoxia) of residence at high altitude or other conditions is generally thought to be beneficial in terms of increasing tissue oxygen supply. However, the extreme polycythemia and accompanying increased mortality due to heart failure in chronic mountain sickness most likely reduces fitness. Tibetan highlanders have adapted to high altitude, possibly in part via the selection of genetic variants associated with reduced polycythemic response to hypoxia. In contrast, high-altitude-adapted Quechua- and Aymara-speaking inhabitants of the Andean Altiplano are not protected from high-altitude polycythemia in the same way, yet they exhibit other adaptive features for which the genetic underpinnings remain obscure. Here, we used whole-genome sequencing to scan high-altitude Andeans for signals of selection. The genes showing the strongest evidence of selection-including BRINP3, NOS2, and TBX5-are associated with cardiovascular development and function but are not in the response-to-hypoxia pathway. Using association mapping, we demonstrated that the haplotypes under selection are associated with phenotypic variations related to cardiovascular health. We hypothesize that selection in response to hypoxia in Andeans could have vascular effects and could serve to mitigate the deleterious effects of polycythemia rather than reduce polycythemia itself. Copyright © 2017. Published by Elsevier Inc.

  13. Organochlorine compounds in soils and sediments of the mountain Andean Lakes

    International Nuclear Information System (INIS)

    Borghini, Francesca; Grimalt, Joan O.; Sanchez-Hernandez, Juan C.; Barra, Ricardo; Garcia, Carlos J. Torres; Focardi, Silvano

    2005-01-01

    Semi-volatile organochlorine compounds (OC) were analyzed in remote Andean soils and lake sediments. The sampling sites covered a wide latitudinal gradient from 18 deg. S to 46 deg. S along Chile and an altitudinal gradient (10-4500 m). The concentrations were in the order of background levels, involving absence of major pollution sources in the high mountain areas. Significant correlations were found between log-transformed concentrations of hexachlorobenzene, α- and γ-hexachlorocyclohexane in soils and total organic content (TOC). In addition, TOC-normalized concentrations of the most volatile OC showed a significant linear dependence with air temperature. This good agreement points to temperature as a significant factor for the retention of long range transported OC in remote ecosystems such as the Andean mountains, although other variables should not be totally excluded. The highest concentrations of OCs were achieved in the sites located at highest altitude and lowest temperature of the dataset. - Distribution of persistent organochlorine compounds in the Andean Mountain lakes is influenced by temperature

  14. Agronomic performance and stability of andean common bean lines with white grains in Brazil

    Directory of Open Access Journals (Sweden)

    Helton Santos Pereira

    2014-06-01

    Full Text Available This work evaluated the effect of genotype by environment interaction in Andean common bean lines with white grains, in Central Southern Brazil, to identify lines with high agronomic performance, stability and adaptability, aiming to meet domestic demand and to increase the Brazilian participation in the foreign market of common bean. Nineteen trials with twelve Andean lines were conducted in 2007, 2008 and 2009, in Central Southern Brazil. Grain yield and other agronomic traits were evaluated. Data were subjected to analysis of variance and of adaptability/stability using Annicchiarico and modified AMMI methods. Significant differences were found between lines for all traits evaluated. Genotype by environment interaction was important for lines with Andean origin and white seed. The utilization of weighted mean of absolute scores and yield with the AMMI results enabled the identification of the most stable and adapted lines. Lines Poroto Alubia, CNFB 16211, Ouro Branco and WAF 160 were stable and adapted, using both methods. CNFB 16211 line presented high agronomic performance, stability and adaptability and therefore this line may be a new cultivar. USWA 70 and WAF 75 lines presented grain size similar to that required by the foreign market and superior to the Brazilian cultivars, besides favorable agronomic traits, and thus these lines may be indicated as new cultivars.

  15. Pathogenicity evaluation of native isolates of entomopathogenic fungí against andean weevil, Premnotrypes vorax (Hustache

    Directory of Open Access Journals (Sweden)

    Giselle Rivera

    2001-07-01

    Full Text Available The main goal of this study was pathogenicity evaluation of Beauveria bassiana and Metarhizium anisopliae native isolates, obtained from natural habitats and stocked potato, against potato andean weevil, Premnotrypes vorax (Hustache (COLEOPTERA: Curculionidae, and important potato insect pest in Colombia and other Andean countries. Patogenicity was determined by laboratory bioassays, using either reared insects or field captured insects. Insect rearing data are presented. Pathogenicity evaluation was express as mortality against time, estimating LT50 and LT90 for all the fungal isolates, and mortality against spore concentration, estimating CL50 for two selected isolates. In all cases, total mortality percentils were above 45%. Differences between reared and field captured insects were evident. According to obtained data one of the fungal isolates: B. bassiana 9770, obtained from R vorax larva (TL50: 4.7 days - 9.8 days; TL90: 14.1 days - 20.8 days; CL50:7.03 x 104spores/ml appears as a promisory fungal isolate for further studies. Out of this study, differences in the andean weevil, P. vorax, adult mortality, with regard to entomopathogenic fungal isolate and insect origin were manifest.

  16. The TF1 Radio Astronomy Working Group in the Andean ROAD: goals and challenges for 2025

    Science.gov (United States)

    Chaparro Molano, G.

    2017-07-01

    Since the creation of the Andean Regional Office of Astronomy for Development (OAD) of the International Astronomical Union, one of the main goals has been to foster a scientific culture of radio astronomy in countries of the central and northern Andes (Bolivia, Colombia, Ecuador, Perú, and Venezuela). For this reason, Andean ROAD Task Force 1 (Research and Education in Universities) created the Radio Astronomy Working Group to set a path along which collaborative endeavors can grow and yield scientific results. The first official meeting of the Working Group took place in Bogotá, Colombia during the 2nd Astronomá en los Andes Workshop (2015) where scientists actively developing projects in radio astronomy set goals for the near future, such as improving mobility for researchers and students, developing collaborations in related areas such as engineering and data science, and building transnational collaborations aiming at developing VLBI across the countries of the Andean ROAD and beyond. In this poster, I present current projects and associated research groups (ROAS - Perú, SiAMo - Colombia, Alfa-Orion UTP - Colombia, RAIG - Chile) and discuss goalposts and current challenges in the development of transnational radioastronomical projects. As a case study, I present the development and early astronomical results of the privately funded UECCI 4m Radio Telescope for 21 cm line observations in Bogotá, Colombia.

  17. Preliminary phylogenetic analysis of the Andean clade and the placement of new Colombian blueberries (Ericaceae, Vaccinieae

    Directory of Open Access Journals (Sweden)

    Paola Pedraza-Penalosa

    2015-04-01

    Full Text Available The blueberry tribe Vaccinieae (Ericaceae is particularly diverse in South America and underwent extensive radiation in Colombia where many endemics occur. Recent fieldwork in Colombia has resulted in valuable additions to the phylogeny and as well in the discovery of morphologically noteworthy new species that need to be phylogenetically placed before being named. This is particularly important, as the monophyly of many of the studied genera have not been confirmed. In order to advance our understanding of the relationships within neotropical Vaccinieae and advice the taxonomy of the new blueberry relatives, here we present the most comprehensive phylogenetic analysis for the Andean clade. Anthopterus, Demosthenesia, and Pellegrinia are among the putative Andean genera recovered as monophyletic, while other eight Andean genera were not. The analyses also showed that genera that have been traditionally widely defined are non-monophyletic and could be further split into more discrete groups. Four newly discovered Colombian Vaccinieae are placed in the monophyletic Satyria s.s. and the Psammisia I clade. Although these new species are endemic to the Colombian Western Cordillera and Chocó biogeographic region and three are not known outside of Las Orquídeas National Park, they do not form sister pairs.

  18. Estimating Aboveground Biomass and Carbon Stocks in Periurban Andean Secondary Forests Using Very High Resolution Imagery

    Directory of Open Access Journals (Sweden)

    Nicola Clerici

    2016-07-01

    Full Text Available Periurban forests are key to offsetting anthropogenic carbon emissions, but they are under constant threat from urbanization. In particular, secondary Neotropical forest types in Andean periurban areas have a high potential to store carbon, but are currently poorly characterized. To address this lack of information, we developed a method to estimate periurban aboveground biomass (AGB—a proxy for multiple ecosystem services—of secondary Andean forests near Bogotá, Colombia, based on very high resolution (VHR GeoEye-1, Pleiades-1A imagery and field-measured plot data. Specifically, we tested a series of different pre-processing workflows to derive six vegetation indices that were regressed against in situ estimates of AGB. Overall, the coupling of linear models and the Ratio Vegetation Index produced the most satisfactory results. Atmospheric and topographic correction proved to be key in improving model fit, especially in high aerosol and rugged terrain such as the Andes. Methods and findings provide baseline AGB and carbon stock information for little studied periurban Andean secondary forests. The methodological approach can also be used for integrating limited forest monitoring plot AGB data with very high resolution imagery for cost-effective modelling of ecosystem service provision from forests, monitoring reforestation and forest cover change, and for carbon offset assessments.

  19. Adding seismic broadband analysis to characterize Andean backarc seismicity in Argentina

    Science.gov (United States)

    Alvarado, P.; Giuliano, A.; Beck, S.; Zandt, G.

    2007-05-01

    Characterization of the highly seismically active Andean backarc is crucial for assessment of earthquake hazards in western Argentina. Moderate-to-large crustal earthquakes have caused several deaths, damage and drastic economic consequences in Argentinean history. We have studied the Andean backarc crust between 30°S and 36°S using seismic broadband data available from a previous ("the CHARGE") IRIS-PASSCAL experiment. We collected more than 12 terabytes of continuous seismic data from 22 broadband instruments deployed across Chile and Argentina during 1.5 years. Using free software we modeled full regional broadband waveforms and obtained seismic moment tensor inversions of crustal earthquakes testing for the best focal depth for each event. We also mapped differences in the Andean backarc crustal structure and found a clear correlation with different types of crustal seismicity (i.e. focal depths, focal mechanisms, magnitudes and frequencies of occurrence) and previously mapped terrane boundaries. We now plan to use the same methodology to study other regions in Argentina using near-real time broadband data available from the national seismic (INPRES) network and global seismic networks operating in the region. We will re-design the national seismic network to optimize short-period and broadband seismic station coverage for different network purposes. This work is an international effort that involves researchers and students from universities and national government agencies with the goal of providing more information about earthquake hazards in western Argentina.

  20. Production and use of the pastures of the Colombia high Andean areas

    International Nuclear Information System (INIS)

    Lotero Cadavid, J.

    1993-01-01

    A relationship of the most common pastures is made in the Andean high area between the 2000 and 3000 m.s.n.m. and of their native vegetable associations, as well as of the invaders plants and overgrowths. The gramineae germoplasm and leguminous is indicated that has been proven lower those conditions. Data of yield average of dry matter are presented for native and introduced grasses of cold climate in Colombia. Equally it is indicated the daily earnings by animal, the load capacity and the animal production with different fertilization systems and some parameters of productivity are shown of gramineous and leguminous introduced in the high areas of Colombia. The nutritious value of gramineous and leguminous of cold climate and its chemical composition are made. A description is made of the ecological areas of the Andean high area and the pastures types that prevail in them. The factors are described that they impact in the degree of deterioration of the pastures like the environment, the same grass, the handling, the livestock, the type of exploitation, the holding of the earth and the administration. The agricultural production systems are mentioned that are associate the Andean pastures, as well as the main obstacles to increase the production of the systems and pastures and their possible solutions

  1. Construction and evaluation of the immune protection of a recombinant divalent protein composed of the MrpA from MR/P fimbriae and flagellin of Proteus mirabilis strain against urinary tract infection.

    Science.gov (United States)

    Habibi, Mehri; Asadi Karam, Mohammad Reza; Bouzari, Saeid

    2018-04-01

    Urinary tract infections (UTI) caused by Proteus mirabilis are prevalent among the catheterized patients. There is no effective vaccine to reduce the frequency of UTIs caused by P. mirabilis. In the present study, the immune responses and effectiveness of different combinations of MrpA and flagellin (FliC) of P. mirabilis were assessed intranasally in the mice model. The addition of FliC as adjuvant to MrpA in fusion form significantly raised the mucosal IgA and cellular (IFN-γ and IL-17) responses and maintained the serum IgG responses for 180 days after the first vaccination. Furthermore, MrpA in fusion form with FliC significantly increased the systemic, mucosal and IFN-γ responses of the FliC alone. In a bladder challenge assay with P. mirabilis, the fusion MrpA.FliC and the mixture of MrpA and FliC significantly decreased the colony count of the bacteria in the bladder and kidneys of mice in comparison to the control mice. It suggests a complex of the systemic, mucosal and cellular responses are needed for protection of the bladder and kidneys against P. mirabilis UTI. In our knowledge, the adjuvant property of the recombinant P. mirabilis flagellin was evaluated for the first time in a vaccine combination administered by an intranasal route. Our results suggest the recombinant flagellin of P. mirabilis could be used as an intranasal adjuvant in combination with other potential antigens against UTIs. Copyright © 2018 Elsevier Ltd. All rights reserved.

  2. Plant biotechnology: transgenic crops.

    Science.gov (United States)

    Shewry, Peter R; Jones, Huw D; Halford, Nigel G

    2008-01-01

    Transgenesis is an important adjunct to classical plant breeding, in that it allows the targeted manipulation of specific characters using genes from a range of sources. The current status of crop transformation is reviewed, including methods of gene transfer, the selection of transformed plants and control of transgene expression. The application of genetic modification technology to specific traits is then discussed, including input traits relating to crop production (herbicide tolerance and resistance to insects, pathogens and abiotic stresses) and output traits relating to the composition and quality of the harvested organs. The latter include improving the nutritional quality for consumers as well as the improvement of functional properties for food processing.

  3. Insights into evolution in Andean Polystichum (Dryopteridaceae) from expanded understanding of the cytosolic phosphoglucose isomerase gene.

    Science.gov (United States)

    Lyons, Brendan M; McHenry, Monique A; Barrington, David S

    2017-07-01

    Cytosolic phosphoglucose isomerase (pgiC) is an enzyme essential to glycolysis found universally in eukaryotes, but broad understanding of variation in the gene coding for pgiC is lacking for ferns. We used a substantially expanded representation of the gene for Andean species of the fern genus Polystichum to characterize pgiC in ferns relative to angiosperms, insects, and an amoebozoan; assess the impact of selection versus neutral evolutionary processes on pgiC; and explore evolutionary relationships of selected Andean species. The dataset of complete sequences comprised nine accessions representing seven species and one hybrid from the Andes and Serra do Mar. The aligned sequences of the full data set comprised 3376 base pairs (70% of the entire gene) including 17 exons and 15 introns from two central areas of the gene. The exons are highly conserved relative to angiosperms and retain substantial homology to insect pgiC, but intron length and structure are unique to the ferns. Average intron size is similar to angiosperms; intron number and location in insects are unlike those of the plants we considered. The introns included an array of indels and, in intron 7, an extensive microsatellite array with potential utility in analyzing population-level histories. Bayesian and maximum-parsimony analysis of 129 variable nucleotides in the Andean polystichums revealed that 59 (1.7% of the 3376 total) were phylogenetically informative; most of these united sister accessions. The phylogenetic trees for the Andean polystichums were incongruent with previously published cpDNA trees for the same taxa, likely the result of rapid evolutionary change in the introns and contrasting stability in the exons. The exons code a total of seven amino-acid substitutions. Comparison of non-synonymous to synonymous substitutions did not suggest that the pgiC gene is under selection in the Andes. Variation in pgiC including two additional accessions represented by incomplete sequences

  4. Delineating priority habitat areas for the conservation of Andean bears in northern Ecuador

    Science.gov (United States)

    Peralvo, M.F.; Cuesta, F.; Van Manen, F.

    2005-01-01

    We sought to identify priority areas for the conservation of Andean bear (Tremarctos ornatus) habitat in the northern portion of the eastern Andean cordillera in Ecuador. The study area included pa??ramo and montane forest habitats within the Antisana and Cayambe-Coca ecological reserves, and unprotected areas north of these reserves with elevations ranging from 1,800 to 4,300 m. We collected data on bear occurrence along 53 transects during 2000-01 in the Oyacachi River basin, an area of indigenous communities within the Cayambe-Coca Ecological Reserve. We used those data and a set of 7 environmental variables to predict suitability of Andean bear habitat using Mahalanobis distance, a multivariate measure of dissimilarity. The Mahalanobis distance values were classified into 5 classes of habitat suitability and generalized to a resolution of 1,650-m ?? 1,650-m grid cells. Clusters of grid cells with high suitability values were delineated from the generalized model and denned as important habitat areas (IHAs) for conservation. The IHAs were ranked using a weighted index that included factors of elevation range, influence from disturbed areas, and current conservation status. We identified 12 IHAs, which were mainly associated with pa??ramo and cloud forest habitats; 2 of these areas have high conservation priorities because they are outside existing reserves and close to areas of human pressure. The distribution of the IHAs highlighted the role of human land use as the main source of fragmentation of Andean bear habitat in this region, emphasizing the importance of preserving habitat connectivity to allow the seasonal movements among habitat types that we documented for this species. Furthermore, the existence of areas with high habitat suitability close to areas of intense human use indicates the importance of bear-human conflict management as a critical Andean bear conservation strategy. We suggest that a promising conservation opportunity for this species is

  5. Captivity Shapes the Gut Microbiota of Andean Bears: Insights into Health Surveillance

    Directory of Open Access Journals (Sweden)

    Andrea Borbón-García

    2017-07-01

    Full Text Available The Andean bear is an endemic species of the tropical Andes who has an almost exclusively plant-based diet. Since herbivorous mammals do not carry enzymes for fiber degradation, the establishment of symbiosis with cellulolytic microorganisms in their gastrointestinal (GI tract is necessary to help them fulfill their nutritional needs. Furthermore, as described for other mammals, a stable, diverse, and balanced gut microbial composition is an indicator of a healthy status of the host; under disturbances this balance can be lost, leading to potential diseases of the host. The goal of this study was to describe the gut microbiota of wild and captive Andean bears and determine how habitat status influences the composition and diversity of the gut symbiotic community. Fecal samples from wild (n = 28 and captive (n = 8 Andean bears were collected in “Reserva Pantano de Martos” and “Fundación Bioandina”, Colombia. Composition and diversity analyses were performed using amplicons from the V4 region of the 16S rDNA gene sequenced using the Ion PGM platform. PICRUSt algorithm was applied to predict the gene content of the gut microbiome of wild and captive Andean bears. A total of 5,411 and 838 OTUs were identified for wild and captive bears, respectively. Captive bears contained a lower number of bacterial phyla (n = 7 compared to wild individuals (n = 9. Proteobacteria (59.03% and Firmicutes (14.03% were the phyla that contributed the most to differences between wild and captive bears (overall dissimilarity = 87.72%. At family level, Enterobacteriaceae drove the main differences between the two groups (13.7%. PICRUSt metagenomics predictions suggested a similar pattern of relative abundance of gene families associated with the metabolism of carbohydrates across samples in wild individuals, despite the taxonomic differences of their gut microbiota. Captivity alters the availability and diversity of food resources, which likely reduces microbiota

  6. Transitions between Andean and Amazonian centers of endemism in the radiation of some arboreal rodents

    Science.gov (United States)

    2013-01-01

    Background The tropical Andes and Amazon are among the richest regions of endemism for mammals, and each has given rise to extensive in situ radiations. Various animal lineages have radiated ex situ after colonizing one of these regions from the other: Amazonian clades of dendrobatid frogs and passerine birds may have Andean ancestry, and transitions from the Amazon to Andes may be even more common. To examine biogeographic transitions between these regions, we investigated the evolutionary history of three clades of rodents in the family Echimyidae: bamboo rats (Dactylomys-Olallamys-Kannabateomys), spiny tree-rats (Mesomys-Lonchothrix), and brush-tailed rats (Isothrix). Each clade is distributed in both the Andes and Amazonia, and is more diverse in the lowlands. We used two mitochondrial (cyt-b and 12S) and three nuclear (GHR, vWF, and RAG1) markers to reconstruct their phylogenetic relationships. Tree topologies and ancestral geographic ranges were then used to determine whether Andean forms were basal to or derived from lowland radiations. Results Four biogeographic transitions are identified among the generic radiations. The bamboo rat clade unambiguously originated in the Amazon ca. 9 Ma, followed by either one early transition to the Andes (Olallamys) and a later move to the Amazon (Dactylomys), or two later shifts to the Andes (one in each genus). The Andean species of both Dactylomys and Isothrix are sister to their lowland species, raising the possibility that highland forms colonized the Amazon Basin. However, uncertainty in their reconstructed ancestral ranges obscures the origin of these transitions. The lone Andean species of Mesomys is confidently nested within the lowland radiation, thereby indicating an Amazon-to-Andes transition ca. 2 Ma. Conclusions Differences in the timing of these biogeographic transitions do not appear to explain the different polarities of these trees. Instead, even within the radiation of a single family, both Andean and

  7. Cover crops support ecological intensification of arable cropping systems

    Science.gov (United States)

    Wittwer, Raphaël A.; Dorn, Brigitte; Jossi, Werner; van der Heijden, Marcel G. A.

    2017-02-01

    A major challenge for agriculture is to enhance productivity with minimum impact on the environment. Several studies indicate that cover crops could replace anthropogenic inputs and enhance crop productivity. However, so far, it is unclear if cover crop effects vary between different cropping systems, and direct comparisons among major arable production systems are rare. Here we compared the short-term effects of various cover crops on crop yield, nitrogen uptake, and weed infestation in four arable production systems (conventional cropping with intensive tillage and no-tillage; organic cropping with intensive tillage and reduced tillage). We hypothesized that cover cropping effects increase with decreasing management intensity. Our study demonstrated that cover crop effects on crop yield were highest in the organic system with reduced tillage (+24%), intermediate in the organic system with tillage (+13%) and in the conventional system with no tillage (+8%) and lowest in the conventional system with tillage (+2%). Our results indicate that cover crops are essential to maintaining a certain yield level when soil tillage intensity is reduced (e.g. under conservation agriculture), or when production is converted to organic agriculture. Thus, the inclusion of cover crops provides additional opportunities to increase the yield of lower intensity production systems and contribute to ecological intensification.

  8. Soil organic matter dynamics at the paramo and puna highlands in the Andean mountains

    Science.gov (United States)

    Ángeles Muñoz, M.; Faz, Ángel; Mermut, Ahmet R.; Zornoza, Raúl

    2014-05-01

    Mountains and uplands represent the most diverse and fragile ecosystems in the world, cover about 20% of the terrestrial surface and are distributed across all continents and major ecoregions. The Andean Plateau is the main mountain range of the American continent and one of the largest in the world with more than 7,500 km. The soil organic matter is a corner stone in the fertility management of the Andean agriculture as well as in the erosion control. However, its role is still much unknown in these ecosystems. Moreover, the influence of current global climatic change on soil organic C reservoirs and dynamics is still not clearly understood. The aim of this work was to review the soil C dynamics and the implication of the soil organic matter in the fertility management, erosion control, conservation of biodiversity and global climate change to improve the knowledge on the mountain Andean highlands. Climate, landscape, soil C pools, biomass and management were studied. In general, the Andean climate is affected by three main factors: ocean currents, winds and orography characterized by an abrupt topography. The entire Andean belt is segmented into the Northern, Central and Southern Andes. Northern Andes are called paramo and are characterized by humid climate while Central and Southern Andes dryer zones are called puna. Most of the region is tectonically and volcanically active. Sedimentary rocks predominated in the paramo while sedimentary, igneous and metamorphic ones prevailed in the puna. The most common soils were Andosols, Regosols, Umbrisols and Histosols. The cold and wet climate and the low atmospheric pressure favored organic matter accumulation in the soil. The accumulation of organic matter is further enhanced by the formation of organomineral complexes strongly resistant to the microbial breakdown mainly in the paramo. High organic C contents were observed in the paramo (10%) oppositely to the low contents found in the dryer puna (1%). The C/N ratio

  9. Identifying the origin of underground water in the Andean Cordillera

    International Nuclear Information System (INIS)

    Rodriguez, C.O.

    1979-01-01

    Two problems associated with groundwater flow were fully identified by the application of geochemical techniques. The first was related to the Palacio tunnel, where infiltration of 400 litres/min caused caving-in and other problems which held up construction for several months. To anticipate further problems and to plan the requisite engineering work, a study was carried out to locate the origin of the groundwater responsible for these difficulties. Water samples were taken from the four possible sources: a pool, a series of large fissures, superficial fluvioglacial deposits and the Guadalupe sandstone which crops out one kilometre from the cave-in site. After correlation of the results of chemical analyses with geological and hydrological information about the area, the conclusion was reached that the groundwater in question flows from the Guadalupe sandstone through fractures and secondary openings. The second problem studied was that of the immense Quebradablanca landslide caused by infiltration of water into and percolation through alluvial deposits. A drainage system had already been designed to intercept and collect direct infiltration water above the alluvium, thereby preventing the latter from becoming saturated. The present study was carried out to determine whether there was groundwater flow from the rocks adjacent to the alluvium. The results of the geochemical analysis indicate that most of the water does in fact come from the adjacent hard rock rather than from direct infiltration of rain-water above the alluvium. This shows that the drainage system designed is insufficient for preventing saturation of the rock and that a more extensive and costly system would be needed to control the groundwater flow. (author)

  10. Development of an operational substrate for ZapA, a metalloprotease secreted by the bacterium Proteus mirabilis

    Directory of Open Access Journals (Sweden)

    B.L. Fernandes

    2000-07-01

    Full Text Available The protease ZapA, secreted by Proteus mirabilis, has been considered to be a virulence factor of this opportunistic bacterium. The control of its expression requires the use of an appropriate methodology, which until now has not been developed. The present study focused on the replacement of azocasein with fluorogenic substrates, and on the definition of enzyme specificity. Eight fluorogenic substrates were tested, and the peptide Abz-Ala-Phe-Arg-Ser-Ala-Ala-Gln-EDDnp was found to be the most convenient for use as an operational substrate for ZapA. A single peptide bond (Arg-Ser was cleaved with a Km of 4.6 µM, a k cat of 1.73 s-1, and a catalytic efficiency of 376 (mM s-1. Another good substrate for ZapA was peptide 6 (Abz-Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg-Gln-EDDnp which was cleaved at a single bond (Phe-Ser with a Km of 13.6 µM, a k cat of 3.96 s-1 and a catalytic efficiency of 291 (mM s-1. The properties of the amino acids flanking the scissile bonds were also evaluated, and no clear requirement for the amino acid residue at P1 was found, although the enzyme seems to have a preference for a hydrophobic residue at P2.

  11. Characterisation of a thiamine diphosphate-dependent alpha-keto acid decarboxylase from Proteus mirabilis JN458.

    Science.gov (United States)

    Wang, Biying; Bai, Yajun; Fan, Taiping; Zheng, Xiaohui; Cai, Yujie

    2017-10-01

    Alpha-keto acid decarboxylases can convert keto acids to their corresponding aldehydes, which are often volatile aroma compounds. The gene encoding α-keto acid decarboxylase in Proteus mirabilis JN458 was cloned, and the enzyme overexpressed in Escherichia coli BL21 (DE3), purified in high yield, and characterised. The molecular weight is 62.291kDa by MALDI-TOF MS, and optimum activity at pH 6.0 and 40-50°C. The enzyme is a typical decarboxylase, dependent on thiamine diphosphate and Mg 2+ as cofactors. For the decarboxylation reaction, the enzyme displayed a broad substrate range. Kinetic parameters were determined using 4-methyl-2-oxopentanoic acid, phenyl pyruvate and 3-methyl-2-oxopentanoic acid as substrates. K m and k cat values for phenyl pyruvate were 0.62mM and 77.38s -1 , respectively, and the k cat /K m value was 124.81mM -1 s -1 . The enzyme properties suggest it may act effectively under cheese ripening conditions. Copyright © 2017. Published by Elsevier Ltd.

  12. Cooccurrence of Multiple AmpC β-Lactamases in Escherichia coli, Klebsiella pneumoniae, and Proteus mirabilis in Tunisia.

    Science.gov (United States)

    Chérif, Thouraya; Saidani, Mabrouka; Decré, Dominique; Boutiba-Ben Boubaker, Ilhem; Arlet, Guillaume

    2016-01-01

    Over a period of 40 months, plasmid-mediated AmpC β-lactamases were detected in Tunis, Tunisia, in 78 isolates (0.59%) of Escherichia coli, Klebsiella pneumoniae, and Proteus mirabilis. In 67 isolates, only one ampC gene was detected, i.e., blaCMY-2-type (n = 33), blaACC (n = 23), blaDHA (n = 6) or blaEBC (n = 5). Multiple ampC genes were detected in 11 isolates, with the following distribution: blaMOX-2, blaFOX-3, and blaCMY-4/16 (n = 6), blaFOX-3 and blaMOX-2 (n = 3), and blaCMY-4 and blaMOX-2 (n = 2). A great variety of plasmids carrying these genes was found, independently of the species and the bla gene. If the genetic context of blaCMY-2-type is variable, that of blaMOX-2, reported in part previously, is unique and that of blaFOX-3 is unique and new. Copyright © 2015, American Society for Microbiology. All Rights Reserved.

  13. Sustainable Agriculture: Cover Cropping

    Science.gov (United States)

    Webster, Megan

    2018-01-01

    Sustainable agriculture practices are increasingly being used by farmers to maintain soil quality, increase biodiversity, and promote production of food that is environmentally safe. There are several types of sustainable agriculture practices such as organic farming, crop rotation, and aquaculture. This lesson plan focuses on the sustainable…

  14. Transpiration and crop yields

    NARCIS (Netherlands)

    Wit, de C.T.

    1958-01-01

    Theoretical and practical aspects of the transpiration of crops in the field are discussed and he concludes that the relationship between transpiration and total dry matter production is much less affected by growing conditions than has been supposed. In semi-arid and arid regions, this relationship

  15. Biotechnology Towards Energy Crops.

    Science.gov (United States)

    Margaritopoulou, Theoni; Roka, Loukia; Alexopoulou, Efi; Christou, Myrsini; Rigas, Stamatis; Haralampidis, Kosmas; Milioni, Dimitra

    2016-03-01

    New crops are gradually establishing along with cultivation systems to reduce reliance on depleting fossil fuel reserves and sustain better adaptation to climate change. These biological assets could be efficiently exploited as bioenergy feedstocks. Bioenergy crops are versatile renewable sources with the potential to alternatively contribute on a daily basis towards the coverage of modern society's energy demands. Biotechnology may facilitate the breeding of elite energy crop genotypes, better suited for bio-processing and subsequent use that will improve efficiency, further reduce costs, and enhance the environmental benefits of biofuels. Innovative molecular techniques may improve a broad range of important features including biomass yield, product quality and resistance to biotic factors like pests or microbial diseases or environmental cues such as drought, salinity, freezing injury or heat shock. The current review intends to assess the capacity of biotechnological applications to develop a beneficial bioenergy pipeline extending from feedstock development to sustainable biofuel production and provide examples of the current state of the art on future energy crops.

  16. Future-proof crops

    NARCIS (Netherlands)

    Kissoudis, Christos; Wiel, van de Clemens; Visser, R.G.F.; Linden, van der Gerard

    2016-01-01

    Breeding for stress-resilient crops strongly depends on technological and biological advancements that have provided a wealth of information on genetic variants and their contribution to stress tolerance. In the context of the upcoming challenges for agriculture due to climate change, such as

  17. Mycorrhiza and crop production

    Energy Technology Data Exchange (ETDEWEB)

    Hayman, D S

    1980-10-09

    This article describes recent research with vesicular-arbuscular mycorrhiza, a symbiotic fungus-root association. The suggestion that the symbiotic association may be harnessed to achieve more economical use of phosphate fertilizers is discussed and the results from various test crops are given.

  18. Building crop models within different crop modelling frameworks

    NARCIS (Netherlands)

    Adam, M.Y.O.; Corbeels, M.; Leffelaar, P.A.; Keulen, van H.; Wery, J.; Ewert, F.

    2012-01-01

    Modular frameworks for crop modelling have evolved through simultaneous progress in crop science and software development but differences among these frameworks exist which are not well understood, resulting in potential misuse for crop modelling. In this paper we review differences and similarities

  19. Crop responses to climatic variation

    DEFF Research Database (Denmark)

    Porter, John R.; Semenov, Mikhail A.

    2005-01-01

    The yield and quality of food crops is central to the well being of humans and is directly affected by climate and weather. Initial studies of climate change on crops focussed on effects of increased carbon dioxide (CO2) level and/or global mean temperature and/or rainfall and nutrition on crop...... production. However, crops can respond nonlinearly to changes in their growing conditions, exhibit threshold responses and are subject to combinations of stress factors that affect their growth, development and yield. Thus, climate variability and changes in the frequency of extreme events are important...... for yield, its stability and quality. In this context, threshold temperatures for crop processes are found not to differ greatly for different crops and are important to define for the major food crops, to assist climate modellers predict the occurrence of crop critical temperatures and their temporal...

  20. Salt resistant crop plants

    KAUST Repository

    Roy, Stuart J.

    2014-04-01

    Soil salinity is a major constraint to agriculture. To improve salinity tolerance of crops, various traits can be incorporated, including ion exclusion, osmotic tolerance and tissue tolerance. We review the roles of a range of genes involved in salt tolerance traits. Different tissues and cells are adapted for specific and often diverse function, so it is important to express the genes in specific cell-types and to pyramid a range of traits. Modern biotechnology (marker- assisted selection or genetic engineering) needs to be increasingly used to introduce the correct combination of genes into elite crop cultivars. Importantly, the effects of introduced genes need to be evaluated in the field to determine their effect on salinity tolerance and yield improvement.

  1. Deforestation and benthic indicators: how much vegetation cover is needed to sustain healthy Andean streams?

    Science.gov (United States)

    Iñiguez-Armijos, Carlos; Leiva, Adrián; Frede, Hans-Georg; Hampel, Henrietta; Breuer, Lutz

    2014-01-01

    Deforestation in the tropical Andes is affecting ecological conditions of streams, and determination of how much forest should be retained is a pressing task for conservation, restoration and management strategies. We calculated and analyzed eight benthic metrics (structural, compositional and water quality indices) and a physical-chemical composite index with gradients of vegetation cover to assess the effects of deforestation on macroinvertebrate communities and water quality of 23 streams in southern Ecuadorian Andes. Using a geographical information system (GIS), we quantified vegetation cover at three spatial scales: the entire catchment, the riparian buffer of 30 m width extending the entire stream length, and the local scale defined for a stream reach of 100 m in length and similar buffer width. Macroinvertebrate and water quality metrics had the strongest relationships with vegetation cover at catchment and riparian scales, while vegetation cover did not show any association with the macroinvertebrate metrics at local scale. At catchment scale, the water quality metrics indicate that ecological condition of Andean streams is good when vegetation cover is over 70%. Further, macroinvertebrate community assemblages were more diverse and related in catchments largely covered by native vegetation (>70%). Our results suggest that retaining an important quantity of native vegetation cover within the catchments and a linkage between headwater and riparian forests help to maintain and improve stream biodiversity and water quality in Andean streams affected by deforestation. This research proposes that a strong regulation focused to the management of riparian buffers can be successful when decision making is addressed to conservation/restoration of Andean catchments.

  2. Radiation and crop improvement

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1960-09-15

    The present state of the research was reviewed and its results analyzed at an international scientific Symposium on the Effects of Ionizing Radiations on Seeds and their Significance for Crop Improvement held at Karlsruhe, Federal Republic of Germany, in 1960. The experts began a detailed examination of certain special aspects of the radiobiology of seeds. Some of the topics discussed related to the processes initiated in seeds as a result of irradiation. The influence of environmental factors, such as temperature, humidity and the presence or absence of oxygen, was also evaluated. Variations in the sensitivity to radiation were taken into consideration and ways of modifying the sensitivity were examined. Two sessions were devoted to a study of radiation- and chemically-induced chromosome breakage and reunion. The nature and mechanism of chromosome breakage and reunion area subject of basic importance in all radiobiological studies and naturally constituted one of the main topics of discussion at the Karlsruhe symposium. The symposium discussed the relevance of these basic scientific questions to crop improvement. Whether irradiation itself, without producing any hereditary changes, can stimulate crop yields is a matter of considerable interest. It has been found that in some cases the effect is stimulating, while in others it is inhibitive. A number of experiments were described and an attempt was made to deduce certain principles from the results obtained

  3. Macrophyte Communities of Andean Rivers: Composition and Relation with Environmental Factors

    OpenAIRE

    Alida Marcela Gómez Rodríguez; Luz Teresa Valderrama Valderrama; Carlos A. Rivera-Rondón

    2017-01-01

    Small streams of tropical Andes have been poorly studied. Therefore, there is little information about the structure, dynamics and function of their macrophyte communities. In this research, aquatic plant communities of 18 Andean streams of La Vieja (Quindío) and Otún (Risaralda) river basins were studied; those are some of the basins most affected by anthropic activities in the country. Streams were selected according to their association with the main land’s uses of the region in both basin...

  4. The rigid Andean sliver hypothesis challenged : impact on interseismic coupling on the Chilean subduction zone

    Science.gov (United States)

    Metois, M.

    2017-12-01

    Convergence partitioning between subduction zones and crustal active structures has been widely evidenced. For instance, the convergence between the Indian and Sunda plates is accommodated both by the Sumatra subduction zone and the Great Sumatran strike-slip fault, that defines a narrow sliver. In Cascadia, small-scale rotating rigid blocks bounded by active faults have been proposed (e.g. McCaffrey et al. 2007). Recent advances in geodetic measurements along the South-American margin especially in Ecuador, Peru and Chile and the need for precise determination of the coupling amount on the megathrust interface in particular for seismic hazard assessment, led several authors to propose the existence of large-scale Andean slivers rotating clockwise and counter-clockwise South and North of the Arica bend, respectively (e.g. Chlieh et al. 2011, Nocquet et al. 2014, Métois et al. 2013). In Chile, one single large Andean sliver bounded to the west by the subduction thrust and to the east by the subandean fold-an-thrust belt active front is used to mimic the velocities observed in the middle to far field that are misfitted by elastic coupling models on the megathrust interface alone (Métois et al. 2016). This rigid sliver is supposed to rotate clockwise around a Euler pole located in the South Atlantic ocean, consistently with long-term observed rotations detected by paleomagnetism (e.g. Arriagada et al. 2008). However, recent GPS data acquired in the Taltal area ( 26°S, Klein et al. submitted) show higher than expected middle-field eastward velocities and question the first-order assumption of a rigid Andean sliver. Mis-modeling the fore-arc deformation has a direct impact on the inverted coupling amount and distribution, and could therefore bias significantly the megathrust rupture scenarios. Correctly estimating the current-day deformation of the Andes is therefore required to properly assess for coupling on the plate interface and is challenging since crustal

  5. Physics in the Andean Countries: A Perspective from Condensed Matter, Novel Materials and Nanotechnology

    Science.gov (United States)

    Prieto, P.

    2009-05-01

    We will discuss the current state of R&D in the fields of condensed matter, novel materials, and nanotechnology in the Andean nations. We will initially consider Latin America and the Caribbean (LAC) to then visualize individual developments, as well as those for the region as a whole in these fields of knowledge in each of the nations constituting the Andean Region (Bolivia, Ecuador, Chile, Venezuela, Peru, and Colombia). Based on Science & Technology watch exercises in the countries involved, along with the Iberian American and Inter-American Science & Technology Network of Indicators (Red de indicadores de Ciencia y Tecnolog'ia (RICYT) iberoamericana e interamericana)1, we will reveal statistical data that will shed light on the development in the fields mentioned. As will be noted, total R&D investment in Latin American and Caribbean countries remained constant since 1997. In spite of having reached a general increase in publications without international collaboration in LAC nations, the countries with greatest research productivity in Latin America (Argentina, Mexico, Brazil, and Chile) have strengthened their international collaboration with the United States, France, Germany, and Italy through close links associated with the formation processes of their researchers. Academic and research integration is evaluated through joint authorship of scientific articles, evidencing close collaboration in fields of research. This principle has been used in the creation of cooperation networks among participating nations. As far as networks of research on condensed matter, novel materials, and nanotechnology, the Andean nations have not consolidated a regional network allowing permanent and effective cooperation in research and technological development; as would be expected, given their idiomatic and cultural similarities, their historical background, and geographical proximity, which have been integrating factors in other research areas or socio-economic aspects. This

  6. [Decriminalizing traditional Andean medicine: an interview with Walter Álvarez Quispe].

    Science.gov (United States)

    Quispe, Walter Álvarez; Loza, Carmen Beatriz

    2014-01-01

    Walter Álvarez Quispe, a Kallawaya healer and biomedical practitioner specializing in general surgery and gynecology, presents the struggle of traditional and alternative healers to get their Andean medical systems depenalized between 1960 and 1990. Bolivia was the first country in Latin America and the Caribbean to decriminalize traditional medicine before the proposals of the International Conference on Primary Health Care (Alma-Ata, 1978). The data provided by the interviewee show that the successes achieved, mainly by the Kallawayas, stem from their own independent initiative. These victories are not the result of official policies of interculturality in healthcare, although the successes achieved tend to be ascribed to them.

  7. Inhibition of Urease Enzyme Production and some Other Virulence Factors Expression in Proteus mirabilis by N-Acetyl Cysteine and Dipropyl Disulphide.

    Science.gov (United States)

    Abdel-Baky, Rehab Mahmoud; Ali, Mohamed Abdullah; Abuo-Rahma, Gamal El-Din Ali A; AbdelAziz, Neveen

    2017-01-01

    Proteus mirabilis is one of the important pathogens that colonize the urinary tract and catheters resulting in various complications, such as blockage of the catheters and the formation of infective stones. In this study we evaluated the effect of N-acetyl cysteine (NAC) and dipropyl disulphide on some virulence factors expressed by a Proteus mirabilis strain isolated from a catheterized patient. Antibacterial activity of both compounds was determined by broth microdilution method. Their effect on different types of motility was determined by LB medium with variable agar content and sub-MIC of each drug. Their effect on adherence and mature biofilms was tested by tissue culture plate assay. Inhibitory effect on urease production was determined and supported by molecular docking studies. The minimum inhibitory concentration (MIC) of NAC and dipropyl disulphide was 25 mM and 100 mM, respectively. Both compounds decreased the swarming ability and biofilm formation of the tested isolate in a dose-dependent manner. NAC had higher urease inhibitory activity (IC50 249 ±0.05 mM) than that shown by dipropyl disulphide (IC 50 10±0.2 mM). Results were supported by molecular docking studies which showed that NAC and dipropyl disulphide interacted with urease enzyme with binding free energy of -4.8 and -8.528 kcal/mol, respectively. Docking studies showed that both compounds interacted with Ni ion and several amino acids (His-138, Gly-279, Cysteine-321, Met-366 and His-322) which are essential for the enzyme activity. NAC and dipropyl disulphide could be used in the control of P. mirabilis urinary tract infections.

  8. Studying the Polypropylenimine-G2 (PPI-G2 Dendrimer Performance in Removal of Escherichia coli, Proteus mirabilis, Bacillus subtilis and Staphylococcus aureus from Aqueous Solution

    Directory of Open Access Journals (Sweden)

    Hasan Izanloo

    2015-09-01

    Full Text Available Abstract Background: Dendrimers are a subset of branched structures that have certain structural order. The aim of this study was to investigate the performance of Polypropylenimine-G2 (PPI-G2 dendrimers in removal of Escherichia coli, Proteus mirabilis, Bacillus subtilis and Staphylococcus aureus from aqueous solution. Materials and Methods: In this experimental study, initially dilution of 103 CFU/ml was prepared from each strain of bacteria. Then, different concentrations of dendrimers (0.5, 5, 50 and 500 µg/ml was added to water. In order to determine the efficiency of dendrimers in removal of bacteria, samples were taken at different times (0, 10, 20, 30, 40, 50 and 60 min and were cultured on nutrient agar medium. Samples were incubated for 24 hours at 37 ° C and then the number of colonies was counted. Results: By the increasment of dendrimer concentration and contact time, the number of bacteria in aqueous solution decreased. In times of 40, 50 and 60 minutes, and the concentrations of 50 and 500 µg/ml, all kinds of bacteria in aqueous solution were removed. 0.5 µg/ml of dendrimer concentration had not effect in reducing the number of Escherichia coli and Proteus mirabilis. The effect of dendrimer on gram-negative bacteria was weaker than gram-positive bacteria. Conclusion: Results of this study indicated that PPI-G2 dendrimer is able to remove Escherichia coli, Proteus mirabilis, Staphylococcus aureus and Bacillus subtilis in aqueous solution. However, using dendrimers can be considered as a new approach for drinking water disinfection but it requires further wide range studies.

  9. Molecular screening of antibiotic-resistant determinants among multidrug-resistant clinical isolates of Proteus mirabilis from SouthWest Nigeria.

    Science.gov (United States)

    Alabi, Olumuyiwa Samuel; Mendonça, Nuno; Adeleke, Olufemi Ezekiel; da Silva, Gabriela Jorge

    2017-06-01

    Globally, and particularly in developing countries, the menace of anti-microbial resistance is an accelerating problem. In Nigeria, increase in bacterial resistance has been phenotypically established but due to high cost, few molecular studies have been reported. This study screened for presence of transferable resistance genes and mobile genetic elements (MGEs) such as integron among multi-drug resistant (MDR) P. mirabilis . A total of 108 P. mirabilis strains collected from five tertiary hospitals in SouthWest Nigeria were subjected to antibiotic susceptibility study using disc-diffusion method. Transferable resistance genes and MGEs were amplified using Polymerase chain reaction (PCR) analysis and amplicons sequenced. Varied resistance was observed against all the antibiotics tested. About 56% of the isolates were MDR including those from 0-12 years old children. PCR analysis revealed the presence of aac(6')-Ib (33.3%), plasmid mediated quinolone resistance (PMQR) genes [qnrA (36.7%), acc(6')-Ib-cr (5%)], TEM (48.3%), CTX-M (6.7%) and integrons class 1 (58.3%) and class 2 (26.7%). Sequencing analysis revealed bla TEM-1 , bla CTX-M-15 associated with IS Ecp1 and eight different arrays of gene cassettes: aadA1, aadA1-qacH, aadB-aadA2, aadA5, dfrA7, dfrA15, dfrA17, dfrA17-aadA5 . Transferable resistance genes in association with MGEs are present in Nigerian P. mirabilis thus their potential in disseminating resistance.

  10. Persistence of antibiotic-resistant and -sensitive Proteus mirabilis strains in the digestive tract of the housefly (Musca domestica) and green bottle flies (Calliphoridae).

    Science.gov (United States)

    Wei, Ting; Miyanaga, Kazuhiko; Tanji, Yasunori

    2014-10-01

    Synanthropic flies have been implicated in the rapid dissemination of antibiotic-resistant bacteria and resistance determinants in the biosphere. These flies stably harbor a considerable number of bacteria that exhibit resistance to various antibiotics, but the mechanisms underlying this phenomenon remain unclear. In this study, we investigated the persistence of antibiotic-resistant bacteria in the digestive tract of houseflies and green bottle flies, using Proteus mirabilis as a model microorganism. One resistant strain carried the blaTEM and aphA1 genes, and another carried a plasmid containing qnrD gene. Quantitative PCR and 454 pyrosequencing were used to monitor the relative abundance of the Proteus strains, as well as potential changes in the overall structure of the whole bacterial community incurred by the artificial induction of Proteus cultures. Both antibiotic-resistant and -sensitive P. mirabilis strains persisted in the fly digestive tract for at least 3 days, and there was no significant difference in the relative abundance of resistant and sensitive strains despite the lower growth rate of resistant strains when cultured in vitro. Therefore, conditions in the fly digestive tract may allow resistant strains to survive the competition with sensitive strains in the absence of antibiotic selective pressure. The composition of the fly-associated bacterial community changed over time, but the contribution of the artificially introduced P. mirabilis strains to these changes was not clear. In order to explain these changes, it will be necessary to obtain more information about bacterial interspecies antagonism in the fly digestive tract.

  11. Identification and characterization of new members of the SXT/R391 family of integrative and conjugative elements (ICEs) in Proteus mirabilis.

    Science.gov (United States)

    Bie, Luyao; Wu, Hao; Wang, Xin-Hua; Wang, Mingyu; Xu, Hai

    2017-08-01

    Integrative and conjugative elements (ICEs) are self-transmissible chromosomal mobile elements that play significant roles in the dissemination of antimicrobial resistance genes. Identification of the structures and functions of ICEs, particularly those in pathogens, improves understanding of the dissemination of antimicrobial resistance. This study identified new members of the sulfamethoxazole-trimethoprim (SXT)/R391 family of ICEs that could confer multi-drug resistance in the opportunistic pathogen Proteus mirabilis, characterized their genetic structures, and explored their evolutionary connection with other members of this family of ICEs. Three new members of the SXT/R391 family of ICEs were detected in six of 77 P. mirabilis strains isolated in China: ICEPmiChn2 (one strain), ICEPmiChn3 (one strain) and ICEPmiChn4 (three strains). All three new ICEs harbour antimicrobial resistance genes from diverse origins, suggesting their capability in acquiring foreign genes and serving as important carriers for antimicrobial resistance genes. Structural analysis showed that ICEPmiChn3 is a particularly interesting and unique ICE that has lost core genes involved in conjugation, and could not transfer to other cells via conjugation. This finding confirmed the key roles of these missing genes in conjugation. Further phylogenetic analysis suggested that ICEs in geographically close strains are also connected evolutionarily, and ICEPmiChn3 lost its conjugation cassette from a former mobile ICE. The identification and characterization of the three new members of the SXT/R391 family of ICEs in this work leads to suggestions of core ICE genes essential for conjugation, and extends understanding on the structures of ICEs, evolutionary relationships between ICEs, and the antimicrobial resistance mechanisms of P. mirabilis. Copyright © 2017 Elsevier B.V. and International Society of Chemotherapy. All rights reserved.

  12. Transurethral instillation with fusion protein MrpH.FimH induces protective innate immune responses against uropathogenic Escherichia coli and Proteus mirabilis.

    Science.gov (United States)

    Habibi, Mehri; Asadi Karam, Mohammad Reza; Bouzari, Saeid

    2016-06-01

    Urinary tract infections (UTIs) are among the most common infections in human. Innate immunity recognizes pathogen-associated molecular patterns (PAMPs) by Toll-like receptors (TLRs) to activate responses against pathogens. Recently, we demonstrated that MrpH.FimH fusion protein consisting of MrpH from Proteus mirabilis and FimH from Uropathogenic Escherichia coli (UPEC) results in the higher immunogenicity and protection, as compared with FimH and MrpH alone. In this study, we evaluated the innate immunity and adjuvant properties induced by fusion MrpH.FimH through in vitro and in vivo methods. FimH and MrpH.FimH were able to induce significantly higher IL-8 and IL-6 responses than untreated or MrpH alone in cell lines tested. The neutrophil count was significantly higher in the fusion group than other groups. After 6 h, IL-8 and IL-6 production reached a peak, with a significant decline at 24 h post-instillation in both bladder and kidney tissues. Mice instilled with the fusion and challenged with UPEC or P. mirabilis showed a significant decrease in the number of bacteria in bladder and kidney compared to control mice. The results of these studies demonstrate that the use of recombinant fusion protein encoding TLR-4 ligand represents an effective vaccination strategy that does not require the use of a commercial adjuvant. Furthermore, MrpH.FimH was presented as a promising vaccine candidate against UTIs caused by UPEC and P. mirabilis. © 2016 APMIS. Published by John Wiley & Sons Ltd.

  13. Carbapenems and piperacillin/tazobactam for the treatment of bacteremia caused by extended-spectrum β-lactamase-producing Proteus mirabilis.

    Science.gov (United States)

    Tsai, Hsih-Yeh; Chen, Yen-Hsu; Tang, Hung-Jen; Huang, Chi-Chang; Liao, Chun-Hsing; Chu, Fang-Yeh; Chuang, Yin-Ching; Sheng, Wang-Huei; Ko, Wen-Chien; Hsueh, Po-Ren

    2014-11-01

    This study was intended to delineate the role of carbapenems and piperacillin/tazobactam in treating bacteremia caused by extended-spectrum β-lactamase (ESBL)-producing Proteus mirabilis. We performed a multicenter and retrospective study of the patients with ESBL-producing P. mirabilis bacteremia. The outcomes of the patients treated by piperacillin/tazobactam or a carbapenem for at least 48 hours and the MICs of the prescribed drugs for these isolates were analyzed. Forty-seven patients with available clinical data were included. The overall 30-day mortality rate was 29.8%. All available isolates (n = 44) were susceptible to ertapenem, meropenem, and doripenem, and 95.6% were susceptible to piperacillin/tazobactam; however, only 11.4% of the isolates were susceptible to imipenem. Among the 3 patients infected with isolates exhibiting non-susceptibility to imipenem (MIC ≥2 mg/L) who were treated with imipenem, none died within 28 days. The 30-day (14.3% versus 23.1%, P = 0.65) or in-hospital (19.1% versus 30.8%, P = 0.68) mortality rate of 21 patients treated by a carbapenem was lower than that of 13 treated by piperacillin/tazobactam. However, among those treated by piperacillin/tazobactam, the mortality rate of those infected by the isolates with lower piperacillin/tazobactam MICs (≤0.5/4 mg/L) was lower than that of the isolates with MICs of ≥1/4 mg/L (0%, 0/7 versus 60%, 3/5; P = 0.045). ESBL-producing P. mirabilis bacteremia is associated with significant mortality, and carbapenem therapy could be regarded as the drugs of choice. The role of piperacillin/tazobactam, especially for the infections due to the isolates with an MIC ≤0.5/4 mg/L, warrants more clinical studies. Copyright © 2014 Elsevier Inc. All rights reserved.

  14. Bloodstream infections caused by multi-drug resistant Proteus mirabilis: Epidemiology, risk factors and impact of multi-drug resistance.

    Science.gov (United States)

    Korytny, Alexander; Riesenberg, Klaris; Saidel-Odes, Lisa; Schlaeffer, Fransisc; Borer, Abraham

    2016-01-01

    The prevalence of antimicrobial co-resistance among ESBL-producing Enterobactereaceae is extremely high in Israel. Multidrug-resistant Proteus mirabilis strains (MDR-PM), resistant to almost all antibiotic classes have been described. The aim was to determine the risk factors for bloodstream infections caused by MDR-PM and clinical outcomes. A retrospective case-control study. Adult patients with PM bacteremia during 7 years were identified retrospectively and their files reviewed for demographics, underlying diseases, Charlson Comorbidity Index, treatment and outcome. One hundred and eighty patients with PM-bloodstream infection (BSI) were included; 90 cases with MDR-PM and 90 controls with sensitive PM (S-PM). Compared to controls, cases more frequently were from nursing homes, had recurrent hospital admissions in the past year and received antibiotic therapy in the previous 3 months, were bedridden and suffered from peripheral vascular disease and peptic ulcer disease (p < 0.001). Two-thirds of the MDR-PM isolates were ESBL-producers vs 4.4% of S-PM isolates (p < 0.001, OR = 47.6, 95% CI = 15.9-142.6). In-hospital crude mortality rate of patients with MDR-PM BSI was 37.7% vs 23.3% in those with S-PM BSI (p = 0.0359, OR = 2, 95% CI = 1.4-3.81). PM bacteremia in elderly and functionally-dependent patients is likely to be caused by nearly pan-resistant PM strains in the institution; 51.8% of the patients received inappropriate empiric antibiotic treatment. The crude mortality rate of patients with MDR-PM BSI was significantly higher than that of patients with S-PM BSI.

  15. BIOGAS PRODUCTION FROM CATCH CROPS

    DEFF Research Database (Denmark)

    Molinuevo-Salces, Beatriz; Larsen, Søren U.; Ahring, Birgitte Kiær

    2014-01-01

    -substrate in manure-based biogas plants and the profit obtained from the sale of biogas barely compensates for the harvest costs. A new agricultural strategy to harvest catch crops together with the residual straw of the main crop was investigated to increase the biomass and thereby the methane yield per hectare......Catch crop cultivation combined with its use for biogas production would increase renewable energy production in the form of methane, without interfering with the production of food and fodder crops. The low biomass yield of catch crops is the main limiting factor for using these crops as co...... biomass. Leaving the straw on the field until harvest of the catch crop in the autumn could benefit biogas production due to the organic matter degradation of the straw taking place on the field during the autumn months. This new agricultural strategy may be a good alternative to achieve economically...

  16. Crop yield response to climate change varies with cropping intensity.

    Science.gov (United States)

    Challinor, Andrew J; Parkes, Ben; Ramirez-Villegas, Julian

    2015-04-01

    Projections of the response of crop yield to climate change at different spatial scales are known to vary. However, understanding of the causes of systematic differences across scale is limited. Here, we hypothesize that heterogeneous cropping intensity is one source of scale dependency. Analysis of observed global data and regional crop modelling demonstrate that areas of high vs. low cropping intensity can have systematically different yields, in both observations and simulations. Analysis of global crop data suggests that heterogeneity in cropping intensity is a likely source of scale dependency for a number of crops across the globe. Further crop modelling and a meta-analysis of projected tropical maize yields are used to assess the implications for climate change assessments. The results show that scale dependency is a potential source of systematic bias. We conclude that spatially comprehensive assessments of climate impacts based on yield alone, without accounting for cropping intensity, are prone to systematic overestimation of climate impacts. The findings therefore suggest a need for greater attention to crop suitability and land use change when assessing the impacts of climate change. © 2015 John Wiley & Sons Ltd.

  17. The Crop Journal Calls for Papers

    Institute of Scientific and Technical Information of China (English)

    2015-01-01

    We would like to invite you to submit your latest research accomplishments to The Crop Journal,an international,peer‐reviewed research publication covering all aspects of crop sciences including crop genetics,breeding,agronomy,crop physiology,germplasm resources,grain chemistry,grain storage and processing,crop management practices,crop biotechnology,and biomathematics on a bimonthly basis.

  18. The Crop Journal Call for Papers

    Institute of Scientific and Technical Information of China (English)

    2016-01-01

    We would like to invite you to submit your latest research accomplishments to The Crop Journal,an international,peer‐reviewed research publication covering all aspects of crop sciences including crop genetics,breeding,agronomy,crop physiology,germplasm resources,grain chemistry,grain storage and processing,crop management practices,crop biotechnology,and biomathematics on a bimonthly basis.

  19. The Crop Journal Call for Papers

    Institute of Scientific and Technical Information of China (English)

    2016-01-01

    We would like to invite you to submit your latest research accomplishments to The Crop Journal,an international,peer-reviewed research publication covering all aspects of crop sciences including crop genetics,breeding,agronomy,crop physiology,germplasm resources,grain chemistry,grain storage and processing,crop management practices,crop biotechnology,and biomathematics on a bimonthly basis.

  20. The Crop Journal Calls for Papers

    Institute of Scientific and Technical Information of China (English)

    2016-01-01

    We would like to invite you to submit your latest research accomplishments to The Crop Journal,an international,peer‐reviewed research publication covering all aspects of crop sciences including crop genetics,breeding,agronomy,crop physiology,germplasm resources,grain chemistry,grain storage and processing,crop management practices,crop biotechnology,and biomathematics on a bimonthly basis.

  1. The Crop Journal Calls for Papers

    Institute of Scientific and Technical Information of China (English)

    2015-01-01

    We would like to invite you to submit your latest research accomplishments to The Crop Journal,an international,peer-reviewed research publication covering all aspects of crop sciences including crop genetics,breeding,agronomy,crop physiology,germplasm resources,grain chemistry,grain storage and processing,crop management practices,crop biotechnology,and biomathematics on a bimonthly basis.

  2. GEOGLAM Crop Monitor Assessment Tool: Developing Monthly Crop Condition Assessments

    Science.gov (United States)

    McGaughey, K.; Becker Reshef, I.; Barker, B.; Humber, M. L.; Nordling, J.; Justice, C. O.; Deshayes, M.

    2014-12-01

    The Group on Earth Observations (GEO) developed the Global Agricultural Monitoring initiative (GEOGLAM) to improve existing agricultural information through a network of international partnerships, data sharing, and operational research. This presentation will discuss the Crop Monitor component of GEOGLAM, which provides the Agricultural Market Information System (AMIS) with an international, multi-source, and transparent consensus assessment of crop growing conditions, status, and agro-climatic conditions likely to impact global production. This activity covers the four primary crop types (wheat, maize, rice, and soybean) within the main agricultural producing regions of the AMIS countries. These assessments have been produced operationally since September 2013 and are published in the AMIS Market Monitor Bulletin. The Crop Monitor reports provide cartographic and textual summaries of crop conditions as of the 28th of each month, according to crop type. This presentation will focus on the building of international networks, data collection, and data dissemination.

  3. [Key to chironomid pupal exuviae (Diptera: Chironomidae) of tropical high Andean streams].

    Science.gov (United States)

    Pratt, Narcís; González-Trujillo, Juan David; Ospina-Torres, Rodulfo

    2014-12-01

    The Chironomidae is a cosmopolitan family of Nematoceran flies with more than 20,000 species described. However the diversity of genera and species of the family in the Andean region beyond the 2,000 m.a.s.l are scarcely known. We conducted faunal surveys and biomonitoring research in different streams of Colombia, Ecuador and Peru from May 2005 to October 2011. Based on specimens collections, a taxonomic key was developed to identify pupae and pupal exuviae of 46 genera of midges (Diptera, Chironomidae) collected from streams at high altitude areas on the Andean tropical mountains. We included illustrations and brief taxonomic descriptions for all genera, of which several ones have not yet been formally described; in this latter case we used the nomenclature of Roback & Coffman (1983). For two genera, Cricotopus and Genus 1, keys to the most com- mon morphospecies were provided. Results showed that in this area the chironomid assemblages are dominated by the members of the subfamily Orthocladiinae (22 genera) followed by the Chironominae (13). Six genera of Tanypodinae were identified, while only three and two genera were present from subfamilies Podonominae and Diamesinae. This key may be very useful for both studies about drift in streams, and for biomonitoring purposes.

  4. A multinational Andean genetic and health program: growth and development in an hypoxic environment.

    Science.gov (United States)

    Mueller, W H; Schull, V N; Schull, W J; Soto, P; Rothhammer, F

    1978-07-01

    In 1972 a multidisciplinary study sought to assess the health status of the indigenous peoples of the Department of Arica in northern Chile, the Aymara, and to relate disease, morphological, physiological and biochemical variation, to the wide changes in altitude of the region. Presented here are the morphological changes which accompany age, altitude and ethnicity amoung 1047 children and adults, permanent residents of the coast, sierra and altiplano. At comparable ages, high-altitude residents were shorter, lighter and leaner but with more expansive and rounder chests than sea-level controls. None of these effects was systematically related to ethnicity (Spanish-Aymara surname), although when stature was held constant, children with greater Aymara ancestry had largest chest circumferences and longer bones. These results suggest that (1) altitude confers allometric growth changes (expensive growth of the chest and diminished growth of the structures less related to oxygen transport); and (2) size changes associated with altitude are acquired during development while shape changes may be under genetic control. Altitude appears to account for less of the variation in growth in this relatively homogeneous Chilean sample than has been reported for other Andean samples, suggesting other concomitants confounding the effects of hypoxia in Andean South America.

  5. The Andean Paepalanthus pilosus complex (Eriocaulaceae): a revision with three new taxa

    Science.gov (United States)

    Hensold, Nancy

    2016-01-01

    Abstract A herbarium-based revision is provided for Paepalanthus pilosus and allies, five commonly confused species of cushion plants native to Andean paramo. These are placed in the recircumscribed Paepalanthus subsect. Cryptanthella Suess. The group includes Paepalanthus pilosus, Paepalanthus dendroides, and Paepalanthus lodiculoides. An additional two species and one variety are newly described: Paepalanthus caryonauta, Paepalanthus huancabambensis, and Paepalanthus pilosus var. leoniae. The latter two are Peruvian endemics, while Paepalanthus caryonauta is known from four countries, and has long been confused with other species. An additional, possibly undescribed taxon is noted from the Serrania de Perijá, Colombia. Five new synonyms and three lectotypes are proposed, and the common misapplication of some names is noted. Within the Paepalanthus pilosus complex, species differences were found in timing of peduncle elongation, sex ratio, and leaf, perianth, diaspore and nectary morphology. Ecological differences are suggested by specimen data and a review of ecological literature. Descriptions, photographs and maps are provided for all species, as is a key to the groups of eriocaulaceous cushion plants from Andean South America. PMID:27489483

  6. Composition and diversity of High Andean in the Fauna Production Reserve Chimborazo, Ecuador

    Directory of Open Access Journals (Sweden)

    Jorge Caranqui

    2016-03-01

    Full Text Available The present study inquire the floristic diversity of 9 sampling in four plots of 1 m² of high andean in several locations in the “Reserva de Producción de Fauna Chimborazo”. For the development of this study, we used an adaptation of the method of plots “Gloria”. With coverage (% in each of the plots, Further the diversity indices and similarity with respective analysis were obtained. The data obtained reflect a diversity that can range from medium to low, believe that this is due to anthropogenic activities that have taken place in these ecosistems. With the presence mostly Calamagrostis intermedia, it could establish that the type of vegetation is herbaceous in high andean is higher percentage; is the species that is almost always present in most types of vegetation of the RPF Chimborazo and high dominance that influences the results of low floristic diversity indices was found in the analysis. As a result the most abundant family Asteraceae is well Poaceae.

  7. Two new trans-Andean species of Imparfinis Eigenmann & Norris, 1900 (Siluriformes: Heptapteridae from Colombia

    Directory of Open Access Journals (Sweden)

    Armando Ortega-Lara

    Full Text Available Two new species of Imparfinis are described from the trans-Andean region of Colombia. Imparfinis timana is diagnosed by having longer anal fin base (12.4-15.5% in SL, in combination with long adipose fin (24.6-31.3% in SL, 5-6 gill rakers on the first ceratobranchial, 42-43 vertebrae and additional measurements. Imparfinis usmai is distinguished by the combination of first ray of dorsal fin longest, but not projected as a long filament, long adipose fin (21.1-27.0% in SL, maxillary barbel exceeding pelvic-fin base, 39-40 vertebrae, upper caudal-fin lobe pointed and longer than lower lobe, lower lobe rounded, 7-8 gill rakers on the first ceratobranchial, as well as additional measurements. Imparfinis timana is only known from río Guarapas, a small tributary of the upper course of the río Magdalena. Imparfinis usmai is broadly distributed in the upper basin of ríos Cauca and Magdalena, and in the lower Patía river basin. The restricted distribution of I. nemacheir to trans-Andean drainages (Atrato, Magdalena, and Lago de Maracaibo is also discussed.

  8. Research Priorities for the Conservation and Sustainable Governance of Andean Forest Landscapes

    Directory of Open Access Journals (Sweden)

    Sarah-Lan Mathez-Stiefel

    2017-08-01

    Full Text Available The long-term survival of Andean forest landscapes (AFL and of their capacity to contribute to sustainable development in a context of global change requires integrated adaptation and mitigation responses informed by a thorough understanding of the dynamic and complex interactions between their ecological and social components. This article proposes a research agenda that can help guide AFL research efforts for the next 15 years. The agenda was developed between July 2015 and June 2016 through a series of workshops in Ecuador, Peru, and Switzerland and involved 48 researchers and development experts working on AFL from different disciplinary perspectives. Based on our review of current research and identification of pressing challenges for the conservation and sustainable governance of AFL, we propose a conceptual framework that draws on sustainability sciences and social–ecological systems research, and we identify a set of high-priority research goals and objectives organized into 3 broad categories: systems knowledge, target knowledge, and transformation knowledge. This paper is intended to be a reference for a broad array of actors engaged in policy, research, and implementation in the Andean region. We hope it will trigger collaborative research initiatives for the continued conservation and sustainable governance of AFL.

  9. in crop plants

    Directory of Open Access Journals (Sweden)

    Jan Antoni Rafalski

    2017-05-01

    Full Text Available Most important crop productivity traits, such as yield under normal and environmental stress conditions, are determined by a large number of genes, each with a small phenotypic effect. Genetic improvement of these traits through breeding or genetic engineering has been frustrating researchers in academia and industry. The reasons for this include the complexity of the traits, the difficulty of precise phenotyping and the lack of validated candidate genes. Different approaches to the discovery of the genetic architecture of such traits, such as Genetic Association Mapping and Genomic Selection and their engineering, are expected to yield benefits for farmers and consumers.

  10. Foreland sedimentary record of Andean mountain building during advancing and retreating subduction

    Science.gov (United States)

    Horton, Brian K.

    2016-04-01

    As in many ocean-continent (Andean-type) convergent margins, the South American foreland has long-lived (>50-100 Myr) sedimentary records spanning not only protracted crustal shortening, but also periods of neutral to extensional stress conditions. A regional synthesis of Andean basin histories is complemented by new results from the Mesozoic Neuquén basin system and succeeding Cenozoic foreland system of west-central Argentina (34-36°S) showing (1) a Late Cretaceous shift from backarc extension to retroarc contraction and (2) an anomalous mid-Cenozoic (~40-20 Ma) phase of sustained nondeposition. New detrital zircon U-Pb geochronological results from Jurassic through Neogene clastic deposits constrain exhumation of the evolving Andean magmatic arc, retroarc thrust belt, foreland basement uplifts, and distal eastern craton. Abrupt changes in sediment provenance and distal-to-proximal depositional conditions can be reconciled with a complex Mesozoic-Cenozoic history of extension, post-extensional thermal subsidence, punctuated tectonic inversion involving thick- and thin-skinned shortening, alternating phases of erosion and rapid accumulation, and overlapping igneous activity. U-Pb age distributions define the depositional ages of several Cenozoic stratigraphic units and reveal a major late middle Eocene-earliest Miocene (~40-20 Ma) hiatus in the Malargüe foreland basin. This boundary marks an abrupt shift in depositional conditions and sediment sources, from Paleocene-middle Eocene distal fluviolacustrine deposition of sediments from far western volcanic sources (Andean magmatic arc) and subordinate eastern cratonic basement (Permian-Triassic Choiyoi igneous complex) to Miocene-Quaternary proximal fluvial and alluvial-fan deposition of sediments recycled from emerging western sources (Malargüe fold-thrust belt) of Mesozoic basin fill originally derived from basement and magmatic arc sources. Neogene eastward advance of the fold-thrust belt involved thick

  11. Quaternary Tectonic and Climatic Processes shaping the Central Andean hyperarid forearc (southern Peru)

    Science.gov (United States)

    Audin, Laurence; Benavente, Carlos; Zerathe, Swann; Saillard, Marianne; Hall, Sarah R.; Farber, Daniel L.

    2015-04-01

    Understanding the forearc structure and processes related to Quaternary evolution and uplift of the Western Andean Cordillera remains an outstanding scientific issue. Models of Andean Plateau evolution based on Tertiary volcanic stratigraphy since 5Ma suggest that the deformation was focused along the eastern margin of the plateau and that minimal uplift occurred along the Pacific margin. On the contrary, new tectonic data and Quaternary surface 10Be dating highlight the presence of recently active deformation, incision and alluvial processes within the upper Andean forearc together with a regional uplift of the coastal zone. Additionally, the high obliquity observed in the northern Arica Bend region makes it an ideal target to discuss whether partitioning of the oblique convergence is accommodated by the neotectonic features that dissect the Quaternary forearc. Our goals are both to decipher the Quaternary tectonic and climatic processes shaping the hyperarid forearc along strike and across strike. Finally, we aim to quantify the respective influence of these factors in the overall uplift of the Western Andes. Indeed, sequences of pediment surfaces, landslide products, paleolake deposits and marine terraces found along the oblique Peruvian margin are a unique set of datable markers that can be used to quantify the rates of Quaternary processes. In this study, we focus on the southern Peru hyperarid Atacama area where regional surfaces and tectonic markers (scarps, folds, temporary streams and paleolake levels offsets…) are well preserved for the Quaternary timescale. Numerous landsliding events align on the major fault segments and reflect Plio-Pleistocene climatic and tectonic activity together with filled and strath terraces. As the present day sea-level is one of the highest levels recorded for Quaternary time span, any emerged marine terrace is preserved by tectonic coastal uplift. In particular, the geomorphic and chronologic correlation between marine and

  12. Opening of the Gulf of Guayaquil: quantifying the motion from the trench to the Andean Cordillera

    Science.gov (United States)

    Santana, E.; Dumont, J. F.; Vilema, W.; Pedoja, K.

    2003-04-01

    The Gulf of Guayaquil is a complex pull-apart opened at the south tip of the North Andean Block. The Guayaquil-Caracas Megashear (i.e. Dolores-Guayaquil Megashear) which bounds the North Andean Block to the east crosses the Western Andean Cordillera along the Pallatanga fault, and joins southwestward the Ecuadorian trench. According to paleogeographic data the Gulf of Guayaquil began to open about 9 Ma ago, which is evidenced now by about 100 km offset of the Early Neogene Progresso and Loja basins. A lesser post orogenic offset of about 60 km is observed along the Pallatanga fault, coherent with the offset of the piedmont of the Western Andean Cordillera. The discrepancy suggests a partition of the deformation at the south tip of the North Andean Block, part of the motion being accommodated by other small faults north of the Pallatanga fault zone and resulting in a pull apart motion and subsidence of the Daule-Babahoyo basin. The main curve of the Tallara Arc described by the trend of the trench from north Peru to north Ecuador shows a significant anomaly in front of the Gulf of Guayaquil. A 60 to 70 km minimum right hand deviation of the curve is observed, displayed in three segments delimited by faults. Neotectonic studies in the Santa Clara and Puná Islands located in the Gulf of Guayaquil show a regional E-W shortening during the Pleistocene, characterised by right lateral motion along the Zambapala Cordillera in Puná. Offset of river drainage and morphostructures in South Puná gives evidence of 3 km dextral offset which post-date marine terraces with an estimated age of M.I.S. 9 or 11, giving mean offset rates of 9 mm/y or 6.8 mm/y respectively. The conclusion underlines the following points: (1) These data suggest that the offset rate inside the Gulf of Guayaquil during the Late Quaternary is lower than the mean rate observed since 9 Ma. The apparent slow down may be due to the partition of the deformation of the Gulf of Guayaquil towards the Guayas

  13. The Likelihood of Confusion in the United State Ninth Circuit and the doctrine of Confusable Marks in the Andean Tribunal

    Directory of Open Access Journals (Sweden)

    Francisco José Cabrera Perdomo

    2016-06-01

    Full Text Available The article analysis the most important cases within the jurisdiction of California regarding the trademark infringement and its prerogative of the likelihood of confusion. Finally, it compares the conclusion with the confusable marks theory within the Andean community’s recent cases solving the issue.

  14. Solar UVR-induced DNA damage and inhibition of photosynthesis in phytoplankton from Andean lakes of Argentina

    NARCIS (Netherlands)

    Villafane, VE; Buma, AGJ; Boelen, P; Helbling, EW

    2004-01-01

    During January 1999, studies were carried out in temperate lakes of the Andean region of Argentina (41degreesS, 71degreesW) to determine the short-term effects of solar ultraviolet radiation (UVR, 280-400 nm) upon natural phytoplankton assemblages. Organisms from one 'clear' (Lake Moreno) and two

  15. Phenolic compound contents and antioxidant activity in plants with nutritional and/or medicinal properties form the Peruvian Andean region

    NARCIS (Netherlands)

    Chirinos, R.; Pedreschi Plasencia, R.P.; Rogez, H.

    2013-01-01

    Total phenolic compounds (TPC) and antioxidant activities using different assays (DPPH, ABTS and ORAC) in fruits, grains, leaves, seeds, roots and tubers from 27 different Peruvian Andean plants used in folk medicine or/and as food by the native population were evaluated in order to use these as

  16. Extreme High Prevalence of a Defective Mannose-Binding Lectin (MBL2) Genotype in Native South American West Andean Populations

    DEFF Research Database (Denmark)

    Sandoval, José Raul; Madsen, Hans O; De Stefano, Gianfranco

    2014-01-01

    communities of the Lake Titicaca), but lower frequencies of 0.22 in Junin (Central Andean highland) and Ucayali (Central Amazonian forest), as well as 0.27 and 0.24 in the Congoma and Cayapa/Chachis populations in the Amazonian forest in Ecuador were also observed. Our results suggest that the high prevalence...

  17. Response of the Andean diversity panel to root rot in a root rot nursery in Puerto Rico

    Science.gov (United States)

    The Andean Diversity Panel (ADP) was evaluated under low-fertility and root rot conditions in two trials conducted in 2013 and 2015 in Isabela, Puerto Rico. About 246 ADP lines were evaluated in the root rot nursery with root rot and stem diseases caused predominantly by Fusarium solani, which cause...

  18. Vegetation composition and altitudinal distribution of Andean rain forests in El Angel and Guandera reserves, northern Ecuador

    NARCIS (Netherlands)

    Moscol Olivera, M.C.; Cleef, A.M.

    2009-01-01

    Patterns of vascular plant species composition and structure of the remaining rain forest of the Andean Cordillera in northern Ecuador were studied in two reserves: Guandera and El Angel. Thirty three plots located between 3300 and 3700 in were examined along two altitudinal transects crossing the

  19. Carbon storage and long-term rate of accumulation in high-altitude Andean peatlands of Bolivia

    Science.gov (United States)

    J.A. Hribljan; D.J. Cooper; J. Sueltenfuss; E.C. Wolf; K.A. Heckman; Erik Lilleskov; R.A. Chimner

    2015-01-01

    The high-altitude (4,500+ m) Andean mountain range of north-western Bolivia contains many peatlands. Despite heavy grazing pressure and potential damage from climate change, little is known about these peatlands. Our objective was to quantify carbon pools, basal ages and long-term peat accumulation rates in peatlands in two areas of the arid puna ecoregion of Bolivia:...

  20. Studies on Colombian cryptogams. IIA. Hepaticae – Oil body structure and ecological distribution of selected species of tropical Andean Jungermanniales

    NARCIS (Netherlands)

    Gradstein, S.R.; Cleef, A.M.; Fulford, M.H.

    1977-01-01

    This paper is the second (Florschütz & Florschütz-de Waard 1974) in the series of reports on cryptogams of Colombia, especially the high Andean bryophytes and lichens, in the framework of recent phytosociological and ecological studies in the area by A. M. Cleef and T. van der Hammen (Amsterdam) and

  1. Vegetation composition and altitudinal distribution of Andean rain forests in El Angel and Guandera reserves, northern Ecuador

    NARCIS (Netherlands)

    Moscol Olivera, M.C.; Cleef, A.M.

    2009-01-01

    Patterns of vascular plant species composition and structure of the remaining rain forest of the Andean Cordillera in northern Ecuador were studied in two reserves: Guandera and El Angel. Thirty three plots located between 3300 and 3700 m were examined along two altitudinal transects crossing the

  2. Landscape-scale drivers of glacial ecosystem change in the montane forests of the eastern Andean flank, Ecuador

    NARCIS (Netherlands)

    Loughlin, N.J.D.; Gosling, W.D.; Coe, A.L.; Gulliver, P.; Mothes, P.; Montoya, E.

    2018-01-01

    Understanding the impact of landscape-scale disturbance events during the last glacial period is vital in accurately reconstructing the ecosystem dynamics of montane environments. Here, a sedimentary succession from the tropical montane cloud forest of the eastern Andean flank of Ecuador provides

  3. Contextual Analysis of the Decision To Adopt a Regional Satellite System: The Case of the Andean CONDOR.

    Science.gov (United States)

    Ferreira, Leonardo; Mody, Bella

    Acknowledging the importance of communications in economic development, this paper discusses the rationale behind the decision of the Andean Pact nations of Bolivia, Colombia, Ecuador, Peru, and Venezuela to develop a regional satellite communication system to be known as CONDOR. The application of contextual theorizing to the decision-making…

  4. Space Data for Crop Management

    Science.gov (United States)

    1990-01-01

    CROPIX, Inc., formed in 1984 by Frank Lamb, president of the Eastern Oregon Farming Company, monitors primarily potato crops in a 20,000 square mile area of northern Oregon and central Washington. Potatoes are a high value specialty crop that can be more profitable to the farmer if he has advance knowledge of market conditions, knows when to harvest, and when to take it to market. By processing and collecting data collected by the NASA-developed Landsat Earth Resources survey satellites, Lamb is able to provide accurate information on crop acreage and conditions on a more timely basis than the routine estimates by the USDA. CROPIX uses Landsat data to make acreage estimates of crops, and to calculate a field-by-field vegetative index number. CROPIX then distributes to its customers a booklet containing color-coded maps, an inventory of crops, plus data and graphs on crop conditions and other valuable information.

  5. Introduction of Alley Cropping

    Directory of Open Access Journals (Sweden)

    Sugeng Parmadi

    2004-01-01

    Full Text Available One of the efforts to preserve the sources of vegetarian, soil, and water is to rehabilitate the land and soil conservation. The aim of this rehabilitation is increasing and maintaining the produtivity of the land, so it can be preserved and used optimally. Therefore, it is necessary to a  develop a variety of good soil conservation, such as vegetative method and civil engineering. To find an appropriate technology, so it is necessary to develop some alternatives of soil conservation technique that are mainly implemented at dry land with its slope of more than 15% in the upstream area of discharge. One of the most suitable soil conservation technique today is Alley Cropping. Based on the research (trial and error in some areas, Alley Cropping could really provide a positive result in terms of erotion controlling and running off and maintain the land productivity. In addition, the technique is more easly operated and spends a cheaper cost than making a bench terrace.

  6. The effect of leaf biopesticide Mirabilis jalapa and fungi Metarhizium anisopliae to immune response and mortality of Spodoptera exigua instar IV

    Science.gov (United States)

    Suryani, A. Irma; Anggraeni, Tjandra

    2014-03-01

    Spodoptera exigua is one of insect causing damage in agriculture sector. This insect can be controlled by a natural biopesticide by combining two agents of biological control, biopesticides Mirabilis jalapa and entomopathogenic fungi Metarhizium anisopliae, considered to be virulent toward a wide range of insects. The objective of research was to determine the effect of biopesticides M. jalapa and fungi M. anisopliae against immune system and mortality of S. exigua. This research used a complete randomized block design with five concentrations Mirabilis jalapa and optimum dose of M. anisopliae. A high dose of M. jalapa (0.8% w/v) is the most effective one to decrease total haemocytes especially granulocyt and plasmatocyt (cellular immune) and decrease the concentration of lectin (humoral immune) from S. exigua (p < 0.05). The combination of M. jalapa (0, 8% w/v) and lethal dose of M. anisopliae 2.59 × 107 spore/ml were significant to increase mortality of S. exigua within 48 hours (p < 0.05).

  7. Initiation of Swarming Motility by Proteus mirabilis Occurs in Response to Specific Cues Present in Urine and Requires Excess l-Glutamine

    Science.gov (United States)

    Armbruster, Chelsie E.; Hodges, Steven A.

    2013-01-01

    Proteus mirabilis, a leading cause of catheter-associated urinary tract infection (CaUTI), differentiates into swarm cells that migrate across catheter surfaces and medium solidified with 1.5% agar. While many genes and nutrient requirements involved in the swarming process have been identified, few studies have addressed the signals that promote initiation of swarming following initial contact with a surface. In this study, we show that P. mirabilis CaUTI isolates initiate swarming in response to specific nutrients and environmental cues. Thirty-three compounds, including amino acids, polyamines, fatty acids, and tricarboxylic acid (TCA) cycle intermediates, were tested for the ability to promote swarming when added to normally nonpermissive media. l-Arginine, l-glutamine, dl-histidine, malate, and dl-ornithine promoted swarming on several types of media without enhancing swimming motility or growth rate. Testing of isogenic mutants revealed that swarming in response to the cues required putrescine biosynthesis and pathways involved in amino acid metabolism. Furthermore, excess glutamine was found to be a strict requirement for swarming on normal swarm agar in addition to being a swarming cue under normally nonpermissive conditions. We thus conclude that initiation of swarming occurs in response to specific cues and that manipulating concentrations of key nutrient cues can signal whether or not a particular environment is permissive for swarming. PMID:23316040

  8. Integration of the blaNDM-1 carbapenemase gene into Proteus genomic island 1 (PGI1-PmPEL) in a Proteus mirabilis clinical isolate.

    Science.gov (United States)

    Girlich, Delphine; Dortet, Laurent; Poirel, Laurent; Nordmann, Patrice

    2015-01-01

    To decipher the mechanisms and their associated genetic determinants responsible for β-lactam resistance in a Proteus mirabilis clinical isolate. The entire genetic structure surrounding the β-lactam resistance genes was characterized by PCR, gene walking and DNA sequencing. Genes encoding the carbapenemase NDM-1 and the ESBL VEB-6 were located in a 38.5 kb MDR structure, which itself was inserted into a new variant of the Proteus genomic island 1 (PGI1). This new PGI1-PmPEL variant of 64.4 kb was chromosomally located, as an external circular form in the P. mirabilis isolate, suggesting potential mobility. This is the first known description of the bla(NDM-1) gene in a genomic island structure, which might further enhance the spread of the bla(NDM-1) carbapenemase gene among enteric pathogens. © The Author 2014. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

  9. SALT TOLERANCE OF CROP PLANTS

    OpenAIRE

    Hamdia, M. A; Shaddad, M. A. K.

    2010-01-01

    Several environmental factors adversely affect plant growth and development and final yield performance of a crop. Drought, salinity, nutrient imbalances (including mineral toxicities and deficiencies) and extremes of temperature are among the major environmental constraints to crop productivity worldwide. Development of crop plants with stress tolerance, however, requires, among others, knowledge of the physiological mechanisms and genetic controls of the contributing traits at different pla...

  10. PROPOSING A DIGITAL INFORMATION SYSTEM FOR THE MANAGEMENT AND CONSERVATION OF THE QHAPAQ ÑAN – ANDEAN ROAD SYSTEM

    Directory of Open Access Journals (Sweden)

    G. N. Duperré

    2017-08-01

    Full Text Available The ancient network of roads in the Andean region is one of the most important works of infrastructure in South America. The extensive territory where the main exchanges between their communities were locally performed is previous to the expansion of the Inca Empire. In the year 2014, the region was included on the World Heritage List by the United Nations Educational, Scientific and Cultural Organization. This communication network is the Qhapaq Ñan, Andean Road System. The Incas planned their route in the diverse landscape of the Andes to promote social and economic ties among the Andean communities. The routes originated in the central square of Cusco, creating cross-connections in a wide geographical area. The Tawantinsuyu depended on this sole route to link very distant production and worship centers. The Qhapaq Ñan was the result of a political project. Even nowadays, it continues to articulate the development of cultural traditions in the Andean region. The present contribution analyzes its transcendental importance as a Cultural Heritage and the singularity of its nomination by the UNESCO, as for the first time six countries are sharing common objectives towards guaranteeing its protection. Furthermore, this research explores the sense of timing in Latin American countries and the implicit challenges in the implementation of the new information technologies for the dissemination of information on Main Andean Road and for its conservation. Although many of the countries have already incorporated the necessary digital tools in this matter, we conclude that there is a need to implement an Integrated Digital Model for the coordinated management in the countries that form the region.

  11. Proposing a Digital Information System for the Management and Conservation of the Qhapaq ÑAN - Andean Road System

    Science.gov (United States)

    Duperré, G. N.

    2017-08-01

    The ancient network of roads in the Andean region is one of the most important works of infrastructure in South America. The extensive territory where the main exchanges between their communities were locally performed is previous to the expansion of the Inca Empire. In the year 2014, the region was included on the World Heritage List by the United Nations Educational, Scientific and Cultural Organization. This communication network is the Qhapaq Ñan, Andean Road System. The Incas planned their route in the diverse landscape of the Andes to promote social and economic ties among the Andean communities. The routes originated in the central square of Cusco, creating cross-connections in a wide geographical area. The Tawantinsuyu depended on this sole route to link very distant production and worship centers. The Qhapaq Ñan was the result of a political project. Even nowadays, it continues to articulate the development of cultural traditions in the Andean region. The present contribution analyzes its transcendental importance as a Cultural Heritage and the singularity of its nomination by the UNESCO, as for the first time six countries are sharing common objectives towards guaranteeing its protection. Furthermore, this research explores the sense of timing in Latin American countries and the implicit challenges in the implementation of the new information technologies for the dissemination of information on Main Andean Road and for its conservation. Although many of the countries have already incorporated the necessary digital tools in this matter, we conclude that there is a need to implement an Integrated Digital Model for the coordinated management in the countries that form the region.

  12. The niche and phylogeography of a passerine reveal the history of biological diversification between the Andean and the Atlantic forests.

    Science.gov (United States)

    Trujillo-Arias, Natalia; Dantas, Gisele P M; Arbeláez-Cortés, Enrique; Naoki, Kazuya; Gómez, Maria I; Santos, Fabricio R; Miyaki, Cristina Y; Aleixo, Alexandre; Tubaro, Pablo L; Cabanne, Gustavo S

    2017-07-01

    The Atlantic Forest is separated from the Andean tropical forest by dry and open vegetation biomes (Chaco and Cerrado). Despite this isolation, both rainforests share closely related lineages, which suggest a past connection. This connection could have been important for forest taxa evolution. In this study, we used the Saffron-billed Sparrow (Arremon flavirostris) as a model to evaluate whether the Andean and the Atlantic forests act as a refugia system, as well as to test for a history of biogeographic connection between them. In addition, we evaluated the molecular systematic of intraspecific lineages of the studied species. We modeled the current and past distribution of A. flavirostris, performed phylogeographic analyses based on mitochondrial and nuclear genes, and used Approximate Bayesian Computation (ABC) analyses to test for biogeographic scenarios. The major phylogeographic disjunction within A. flavirostris was found between the Andean and the Atlantic forests, with a divergence that occurred during the Mid-Pleistocene. Our paleodistribution models indicated a connection between these forest domains in different periods and through both the Chaco and Cerrado. Additionally, the phylogeographic and ABC analyses supported that the Cerrado was the main route of connection between these rainforests, but without giving decisive evidence against a Chaco connection. Our study with A. flavirostris suggest that the biodiversity of the Andean and of the Atlantic forests could have been impacted (and perhaps enriched?) by cycles of connections through the Cerrado and Chaco. This recurrent cycle of connection between the Andean and the Atlantic Forest could have been important for the evolution of Neotropical forest taxa. In addition, we discussed taxonomic implications of the results and proposed to split the studied taxon into two full species. Copyright © 2017 Elsevier Inc. All rights reserved.

  13. Osmotic and thermal effects on in situ ATPase activity in permeabilized gill epithelial cells of the fish Gillichthys mirabilis

    Science.gov (United States)

    KÜLtz; Somero

    1995-01-01

    Long-jawed mudsuckers (Gillichthys mirabilis) were acclimated to sea water (SW) at 7 °C, SW at 26 °C or dilute sea water (DSW) at 26 °C for 5 months. Gill cells were isolated and the proportion of mitochondria-rich (MR) cells was determined. The number of cells harvested amounted to 4.7x10(7)±0.6x10(7) to 10.6x10(7)±1.1x10(7) and the yield was between 7.1x10(8)±0.6x10(8) and 10.7x10(8)±1.4x10(8) cells g-1 gill epithelial mass. Cell viability was 96.8±0.4 to 97.8±0.6 %. The number, size and volume of MR cells decreased significantly during DSW acclimation, but did not change during thermal acclimation. The protein content was not influenced by osmotic or thermal acclimation and ranged between 20.0±1.6 and 22.1±1.5 pg cell-1. Using a new method, which is based on the formation of plasma membrane channels by alamethicin, we were able to permeabilize gill cells. For the first time, the Na+/K+-ATPase and H+-ATPase activities of fish gills were determined in intact cells in situ. The activity of both ATPases was dependent on alamethicin concentration (optimum 100 µg mg-1 protein) and on preincubation time (optimum 10 min). The in situ activity of both ATPases was influenced by osmotic, but not thermal, acclimation. A positive linear correlation was found between in situ Na+/K+-ATPase activity and total MR cell volume. However, we show, for the first time, that a negative linear correlation exists between H+-ATPase activity and total MR cell volume, suggesting a localization of H+-ATPase in pavement cells. In permeabilized cells, the activity of both ATPases was 2.6­3.9 times higher than that of crude homogenates and 1.6­2.1 times higher than that of permeabilized homogenate vesicles. We hypothesize that in crude homogenates three-quarters of Na+/K+-ATPase and two-thirds of H+-ATPase activity are not detectable both because of a mixture of inside-out and right-side-out vesicles and because of the disruption of membrane and enzyme integrity.

  14. Evaluation of Aqua crop Model to Predict Crop Water Productivity

    International Nuclear Information System (INIS)

    Mohd Noor Hidayat Adenan; Faiz Ahmad; Shyful Azizi Abdul Rahman; Abdul Rahim Harun; Khairuddin Abdul Rahim

    2015-01-01

    Water and nutrient are critical inputs for crop production, especially in meeting challenges from increasing fertilizer cost and irregular water availability associated with climate change. The Land and Water Division of Food and Agriculture Organization of the United Nations (FAO) has developed Aqua Crop, an integrated application software to simulate the interactions between plant, water and soil. Field management and irrigation management are the factors that need to be considered since it affects the interactions. Four critical components are needed in the Aqua Crop model, viz. climate, crop, field management and soil conditions. In our case study, climate data from rice field in Utan Aji, Kangar, Perlis was applied to run a simulation by using AquaCrop model. The rice crop was also assessed against deficit irrigation schedules and we found that use of water at optimum level increased rice yield. Results derived from the use of the model corresponded conventional assessment. This model can be adopted to help farmers in Malaysia in planning crop and field management to increase the crop productivity, especially in areas where the water is limited. (author)

  15. Evaluation of the effect of MPL and delivery route on immunogenicity and protectivity of different formulations of FimH and MrpH from uropathogenic Escherichia coli and Proteus mirabilis in a UTI mouse model.

    Science.gov (United States)

    Habibi, Mehri; Asadi Karam, Mohammad Reza; Bouzari, Saeid

    2015-09-01

    Urinary tract infections (UTIs) caused by Escherichia coli and Proteus mirabilis are an important cause of morbidity and with the high rate of relapse and spread of multi-drug resistant pathogens, pose a significant public health challenge worldwide. Lack of an efficacious commercial vaccine targeting both uropathogens makes development of a combined vaccine highly desirable. In this study the immunogenicity and protective efficacy of different formulations of FimH of UPEC, MrpH of P. mirabilis and their fusion protein (MrpH.FimH) subcutaneously administered with and without Monophosphoryl lipid A (MPL) adjuvant were evaluated. Our data showed that the subcutaneously administered proteins induced both serum and mucosal IgG, which MPL significantly improved developing a mixed Th1 and Th2 immune response. However, the preparations induced a higher systemic and mucosal IgG and IL-2 levels by this route compared to the intranasal. Immunization of mice with MrpH.FimH fusion with MPL or a mixture of FimH, MrpH and MPL conferred the highest protection of the bladder and kidneys when challenged with UPEC and P. mirabilis in a UTI mouse model. Therefore considering these results MrpH.FimH fusion with MPL administered subcutaneously or intranasally could be a promising vaccine candidate for elimination of UTIs caused by UPEC and P. mirabilis. Copyright © 2015 Elsevier B.V. All rights reserved.

  16. Isolation and Characterization of a Lytic Bacteriophage (vB_PmiS-TH) and Its Application in Combination with Ampicillin against Planktonic and Biofilm Forms of Proteus mirabilis Isolated from Urinary Tract Infection.

    Science.gov (United States)

    Yazdi, Mahsa; Bouzari, Majid; Ghaemi, Ezzat Allah

    2018-01-01

    Proteus mirabilis is one of the most common causes of urinary tract infection (UTI), particularly in patients undergoing long-term catheterization. Phage vB_PmiS-TH was isolated from wastewater with high lytic activity against P. mirabilis (TH) isolated from UTI. The phage had rapid adsorption, a large burst size (∼260 PFU per infected cell), and high stability at a wide range of temperatures and pH values. As analyzed by transmission electron microscopy, phage vB_PmiS-TH had an icosahedral head of ∼87 × 62 nm with a noncontractile tail about 137 nm in length and 11 nm in width. It belongs to the family Siphoviridae. Combination of the phage vB_PmiS-TH with ampicillin had a higher removal activity against planktonic cells of P. mirabilis (TH) than the phage or the antibiotic alone. Combination of the phage at a multiplicity of infection of 100 with a high dose of ampicillin (246 µg/mL) showed the highest biofilm removal activity after 24 h. This study demonstrates that using a combination of phage and antibiotic could be significantly more effective against planktonic and biofilm forms of P. mirabilis (TH). © 2018 S. Karger AG, Basel.

  17. Otojenik Kaynaklı Proteus Mirabilis İnfeksiyonu Sonucu Gelişen Serebellar Apse ile İlişkili Travmatik Olmayan Pnömosefalus Olgusu

    OpenAIRE

    CİHANGİROĞLU, Mustafa; ÇELİK, İlhami; AKDEMİR, İsmail; ARTAŞ, Hakan; AKBULUT, Ayhan

    2008-01-01

    In this case, it was presented a rare cerebellar abscess case due to Proteus mirabilis has an intraparenchymal gas formation at the early stage of cerebritis where gas formation disappeared at the late cerebritis phase of the abscess formation.©2008, Fırat Üniversitesi, Tıp Fakültesi

  18. Integración regional andina en salud Health in Andean regional integration

    Directory of Open Access Journals (Sweden)

    Carlos A. Agudelo

    2007-01-01

    Full Text Available A pesar su historia compartida, los países de la Región Andina presentan una diversidad social y política que genera realidades sanitarias heterogéneas y procesos de integración complejos. Se han dado por décadas procesos generales, como la Asociación Latinoamericana de Libre Comercio y la Asociación Latinoamericana de Integración, o de alcance regional como la Comunidad Andina de Naciones, la Comunidad del Caribe y el Mercado Común Centroamericano. En el campo específico de la salud se cuenta con un instrumento en la Región Andina, el Convenio Hipólito Unánue, creado en 1971. Estos procesos de integración se han concentrado en los aspectos económicos, con base en acuerdos de preferencias arancelarias, los cuales han generado, en el largo plazo, un intercambio comercial apreciable. En el campo de la salud se ha avanzado menos, en términos de procesos que ponen en común experiencias nacionales, conocimientos y capacidades. El análisis de las experiencias de integración en salud muestra que esta depende de las fortalezas de cada país y, en gran parte, de los procesos políticos nacionales.Despite their shared history, the Andean countries are socially and politically diverse, with heterogeneous health realities and complex integration processes. General developments such as the Latin American Free Trade Association and Latin American Integration Association have existed for decades, along with others of a regional scope, like the Andean Community of Nations, Caribbean Community, and Central American Common Market. The health field has a specific instrument in the Andean Region called the Hipólito Unánue Agreement, created in 1971. Integration processes have concentrated on economic aspects, based on preferential customs agreements that have led to an important long-term increase in trade. Less progress has been made in the field of health in terms of sharing national experiences, knowledge, and capabilities. Analysis of

  19. Biosolarization in garlic crop

    Science.gov (United States)

    Fabeiro, Concepcion; Andres, Manuela; Wic, Consuelo

    2014-05-01

    One of the most important limitations of garlic cultivation is the presence of various soil pathogens. Fusarium proliferatum and Sclerotinium cepivorum and nematode Ditilenchus dipsaci cause such problems that prevent the repetition of the crop in the same field for at least 5 -8 years or soil disinfection is necessary. Chemical disinfection treatments have an uncertain future, in the European Union are reviewing their use, due to the effect on the non-pathogenic soil fauna. This situation causes a itinerant cultivation to avoid the limitations imposed by soil diseases, thereby increasing production costs. The Santa Monica Cooperative (Albacete, Spain) requested advice on possible alternative techniques, solarization and biosolarization. For which a trial was conducted to evaluate the effectiveness on the riverside area of the municipality. This place has recently authorized irrigation, which would allow the repeated cultivation of garlic if the incidence of soil diseases and the consequent soil fatigue could be avoided. Additionally, this work will serve to promote the cultivation of organic garlic. Last, but not least, the biosolarization technique allows to use waste from wineries, oil mills and mushroom crops. (Bello et al. 2003). The essay should serve as demonstrative proof for farmers' cooperative members. The specific objective for this first year is to assess, the effect on the global soil biota, on the final garlic production and quality and the effect of biosolarization to control soil pathogens. The trial is set on a cooperative's plot previously cultivated with corn. 5 treatments were set, defined by different amounts of organic matter applied, 7.5, 5, 2.5 kg m -2, a solarized with no organic matter, and a control without any treatment. The plot has inground sprinkler for full coverage with four sprinkler lines demarcating the five bands of differential treatment, randomly arranged. Organic matter was incorporated the August 14, 2013, then thoroughly

  20. Crop Protection in Medieval Agriculture

    NARCIS (Netherlands)

    Zadoks, J.C.

    2013-01-01

    Mediterranean and West European pre-modern agriculture (agriculture before 1600) was by necessity ‘organic agriculture’. Crop protection is part and parcel of this agriculture, with weed control in the forefront. Crop protection is embedded in the medieval agronomy text books but specialised

  1. Potential photosynthesis of crop surfaces.

    NARCIS (Netherlands)

    Wit, de C.T.

    1959-01-01

    A formula for calculating the potential photosynthesis of a closed crop surface is proposed, assuming that the leaves of the crop are not arranged in any definite direction. In the Netherlands, values for potential photosynthesis vary from 290 kg. CH2O/ha./day in June to 50 kg./ha./day in December.

  2. Cassava as an energy crop

    DEFF Research Database (Denmark)

    Kristensen, Søren Bech Pilgaard; Birch-Thomsen, Torben; Rasmussen, Kjeld

    2014-01-01

    of the Attieké cassava variety. Little competition with food crops is likely, as cassava most likely would replace cotton as primary cash crop, following the decline of cotton production since 2005 and hence food security concerns appear not to be an issue. Stated price levels to motivate an expansion of cassava...

  3. Climate Impacts of Cover Crops

    Science.gov (United States)

    Lombardozzi, D.; Wieder, W. R.; Bonan, G. B.; Morris, C. K.; Grandy, S.

    2016-12-01

    Cover crops are planted in agricultural rotation with the intention of protecting soil rather than harvest. Cover crops have numerous environmental benefits that include preventing soil erosion, increasing soil fertility, and providing weed and pest control- among others. In addition to localized environmental benefits, cover crops can have important regional or global biogeochemical impacts by increasing soil organic carbon, changing emissions of greenhouse trace gases like nitrous oxide and methane, and reducing hydrologic nitrogen losses. Cover crops may additionally affect climate by changing biogeophysical processes, like albedo and latent heat flux, though these potential changes have not yet been evaluated. Here we use the coupled Community Atmosphere Model (CAM5) - Community Land Model (CLM4.5) to test how planting cover crops in the United States may change biogeophysical fluxes and climate. We present seasonal changes in albedo, heat fluxes, evaporative partitioning, radiation, and the resulting changes in temperature. Preliminary analyses show that during seasons when cover crops are planted, latent heat flux increases and albedo decreases, changing the evaporative fraction and surface temperatures. Understanding both the biogeophysical changes caused by planting cover crops in this study and the biogeochemical changes found in other studies will give a clearer picture of the overall impacts of cover crops on climate and atmospheric chemistry, informing how this land use strategy will impact climate in the future.

  4. Chemical mutagenesis for crop improvement

    International Nuclear Information System (INIS)

    1986-01-01

    Focusses on methodological aspects for the efficient induction of mutations in crop plants by chemomutagens. Mutagen treatment of barley seeds with ethylmethane sulfonate (EMS) is documented in detail to exemplify procedural phases. Reference is made to safe handling and the prevention of biohazards. Induced biological and genetic effects at various plant generations are documented and the use of mutants for crop improvement is discussed

  5. Genetic Engineering and Crop Production.

    Science.gov (United States)

    Jones, Helen C.; Frost, S.

    1991-01-01

    With a spotlight upon current agricultural difficulties and environmental dilemmas, this paper considers both the extant and potential applications of genetic engineering with respect to crop production. The nonagricultural factors most likely to sway the impact of this emergent technology upon future crop production are illustrated. (JJK)

  6. Archives: African Crop Science Journal

    African Journals Online (AJOL)

    Items 1 - 50 of 99 ... Archives: African Crop Science Journal. Journal Home > Archives: African Crop Science Journal. Log in or Register to get access to full text downloads. Username, Password, Remember me, or Register · Journal Home · ABOUT THIS JOURNAL · Advanced Search · Current Issue · Archives. 1 - 50 of 99 ...

  7. Archives: African Crop Science Journal

    African Journals Online (AJOL)

    Items 51 - 99 of 99 ... Archives: African Crop Science Journal. Journal Home > Archives: African Crop Science Journal. Log in or Register to get access to full text downloads. Username, Password, Remember me, or Register · Journal Home · ABOUT THIS JOURNAL · Advanced Search · Current Issue · Archives. 51 - 99 of 99 ...

  8. ANDEAN COSMOLOGY AND RELIGION: A HISTORICAL DYNAMICS OF ENCOUNTERS, MISSED CONNECTIONS AND REUNIONS

    Directory of Open Access Journals (Sweden)

    Avelar Araujo Santos Junior

    2009-06-01

    Full Text Available Religiosity of contemporary Andean indigenous peoples is constituted by a complex interaction between theology and the original items from the Christianized and ideologies historically produced phenomena such as taxation, interpenetration, removal, syncretism, resistance and reinvention. Embedded in this context was developed as a way of enhancing identity particular worldview is quite representative of their feelings and attitudes about the world, mainly in what it says respect to their territory and their community experiences and patterns of reciprocity in the socialization of traditions. Thus, our proposal in this article is to analyze some of the different elements of the symbolic representation, mythological and ritualistic of these communities, characterized by persistent conflict between a hegemonic dominance of the creative and creative autonomy.

  9. Size class structure, growth rates, and orientation of the central Andean cushion Azorella compacta

    Directory of Open Access Journals (Sweden)

    Catherine Kleier

    2015-03-01

    Full Text Available Azorella compacta (llareta; Apiaceae forms dense, woody, cushions and characterizes the high elevation rocky slopes of the central Andean Altiplano. Field studies of an elevational gradient of A. compacta within Lauca National Park in northern Chile found a reverse J-shape distribution of size classes of individuals with abundant small plants at all elevations. A new elevational limit for A. compacta was established at 5,250 m. A series of cushions marked 14 years earlier showed either slight shrinkage or small degrees of growth up to 2.2 cm yr−1. Despite their irregularity in growth, cushions of A. compacta show a strong orientation, centered on a north-facing aspect and angle of about 20° from horizontal. This exposure to maximize solar irradiance closely matches previous observations of a population favoring north-facing slopes at a similar angle. Populations of A. compacta appear to be stable, or even expanding, with young plants abundant.

  10. "It Is Not Natural Anymore": Nutrition, Urbanization, and Indigenous Identity on Bolivia's Andean Plateau.

    Science.gov (United States)

    Lipus, Adam C; Leon, Juan S; Calle, Susana C; Andes, Karen L

    2018-03-01

    The objective of this article was to characterize how urbanization and indigenous identity shape nutrition attitudes and practices in El Alto, a rapidly urbanizing and predominantly indigenous (Aymara) community on Bolivia's Andean plateau. We took a qualitative ethnographic approach, interviewing health care providers ( n = 11) and conducting focus groups with mothers of young children ( n = 4 focus groups with 25 mothers total [age = 18-43 years, 60% Aymara]). Participants generally described their urban environment as being problematic for nutrition, a place where unhealthy "junk foods" and "chemicals" have supplanted healthy, "natural," "indigenous" foods from the countryside. Placing nutrition in El Alto within a broader context of cultural identity and a struggle to harmonize different lifestyles and worldviews, we propose how an intercultural framework for nutrition can harmonize Western scientific perspectives with rural and indigenous food culture.

  11. Attitudes towards Human Papilloma Virus Vaccination in the Latin American Andean Region.

    Science.gov (United States)

    Nwanodi, Oroma

    2017-09-08

    This commentary explores the distribution of human papilloma virus (HPV) and HPV-related diseases, and factors affecting attitudes towards HPV, HPV-related diseases, and HPV vaccination in the Latin American Andean region. Lack of knowledge of HPV, known negative attitudes or incorrect assumptions about HPV, HPV-related diseases, and HPV vaccination provide a basis upon which to develop targeted HPV awareness and preventive health media campaigns. For maximal effect, media campaigns should use the internet, radio, and television to address health care providers, parents, and students. Additional programming can be developed for clinics to use in-house with their clients. Ministries of Education, Finance, and Health all have roles to play to increase national HPV, HPV-related diseases, and HPV vaccination awareness.

  12. The Sabethines of Northern Andean Coffee-Growing Regions of Colombia.

    Science.gov (United States)

    Suaza-Vasco, Juan; López-Rubio, Andrés; Galeano, Juan; Uribe, Sandra; Vélez, Iván; Porter, Charles

    2015-06-01

    Sampling for sabethine mosquitoes occurred intermittently from September 2007 to April 2013 in 17 municipalities, located in 5 departments (divisions) in the northern Andean coffee-growing regions of Colombia. Of the 9 genera within the Sabethini tribe known to occur in the Neotropical region, 6 were encountered including 15 species: Jonhbelkinia ulopus, Limatus durhamii, Sabethes ignotus, Sa. luxodens, Sa. undosus, Shannoniana fluviatilis, Trichoprosopon compressum, Tr. digitatum, Tr. evansae, Tr. pallidiventer s.l., Tr. pallidiventer s.s., Wyeomyia arthrostigma, Wy. oblita, Wy. ulocoma, and Wy. undulata. The species Sa. luxodens and Wy. undulata constitute new records for Colombia. These records broaden the knowledge of this important group that includes some important species related to the arbovirus transmission. Records are from the northern Colombian Andes, a region noted for coffee cultivation and ecotourism.

  13. Attitudes towards Human Papilloma Virus Vaccination in the Latin American Andean Region

    Directory of Open Access Journals (Sweden)

    Oroma Nwanodi

    2017-09-01

    Full Text Available This commentary explores the distribution of human papilloma virus (HPV and HPV-related diseases, and factors affecting attitudes towards HPV, HPV-related diseases, and HPV vaccination in the Latin American Andean region. Lack of knowledge of HPV, known negative attitudes or incorrect assumptions about HPV, HPV-related diseases, and HPV vaccination provide a basis upon which to develop targeted HPV awareness and preventive health media campaigns. For maximal effect, media campaigns should use the internet, radio, and television to address health care providers, parents, and students. Additional programming can be developed for clinics to use in-house with their clients. Ministries of Education, Finance, and Health all have roles to play to increase national HPV, HPV-related diseases, and HPV vaccination awareness.

  14. History aspects and ecology of the biodiversity nor Andean and Amazonic

    International Nuclear Information System (INIS)

    Van der Hammen, Thomas

    2000-01-01

    The mega-biodiversity of the neotropics is type result of the geological and environmental history. A considerable biodiversity, higher than at present, existed in the Miocene in the low-elevation tropics. The progressive upheaval of the Andes created new life zones that were populated by adaptive evolution and immigration from the austral-Antarctic and laurasiatic-holartic regions. The cooling of the earth during the Neogene and the glacial- interglacial cycles of the quaternary, and the consonant changes of temperature and rainfall, in combination with the topography, had a profound effect on vegetation, flora and fauna, the distribution of species and endemism, both in the low tropical area and in the Andean zones. Presently there is a positive relation of species-density with temperature (altitude) and with rainfall, and partly with relative humidity

  15. Scope of fossil energy resources in Mexico and the Andean Group

    International Nuclear Information System (INIS)

    Figueroa Vega, F. de la; Boesl, B.

    1997-01-01

    The article focuses on fossil energy resources in the Andean Group and Mexico, and argues for a dynamic approach to calculating reserves to production ratios. Individual countries are surveyed in terms of estimated reserves of fossil energy, resources, current production rates and future prospects; energy policy options for individual countries are analysed. As a primary objective of energy policy is to ensure security of supply, it is important that that calculations of reserves to production ratios accurately, estimate the desired level of investment in exploration. The calculations need to take into account a variety of parameters, including different energy sources, availability and risk; geographic considerations including proximity, transport, storage capacity and commercial aspects; the competitive and environmental implications of developing indigenous resources; and the use of instruments to establish contingency plans for emergencies

  16. Diversity patterns, environmental drivers and changes in vegetation composition in dry inter-Andean valleys

    DEFF Research Database (Denmark)

    Quintana, Catalina; Girardello, Marco; Barfod, Anders

    2016-01-01

    Aims We studied diversity, patterns of endemism and turnover of vegetation composition in dry inter-Andean valleys (DIAVs) where little is known about the influence of the abiotic drivers controlling plant species composition and occurrences, and the life forms that contribute most to α- and β...... species with low abundance (e.g. 30 individuals ha−1), including four endemic species. Most of the endemic species were locally rare, and most of them were restricted to southern valleys...... results further highlight the influence of disturbance, water availability and low temperature on plant species composition and occurrence. We also found significant, contrasting patterns in responses to environmental drivers, when analyzing our data separately by life form. Our results show...

  17. Collaborative networks and patent production in Andean Community of Nations universities (UCANS, 2005-2015

    Directory of Open Access Journals (Sweden)

    Carlos Enrique Agüero Aguilar

    2017-06-01

    Full Text Available The competitiveness and technological development of a region are measured by the degree of innovation supporting them. The quantity and quality of patents generated and applied in production dynamics serve as an element for evaluation. In this sense, universities play a role as generators and transmitters of knowledge. So it is important to identify the level of their collaboration and the trends in terms of technology application in order to establish future policies for development in this sector. This article identifies the degree of collaboration, types of patents, actors (primary and secondary and dynamics of patents produced at the Andean Community of Nations universities during the period 2005-2015 and present in the European Patent Office database. In conclusion, there is a great disparity between CAN universities regarding patent production, so it is necessary to strengthen the collaborative level among universities in this community. Nevertheless, an increase is seen in the production of patents.

  18. Mercury Contamination in an Indicator Fish Species from Andean Amazonian Rivers Affected by Petroleum Extraction.

    Science.gov (United States)

    Webb, Jena; Coomes, Oliver T; Mainville, Nicolas; Mergler, Donna

    2015-09-01

    Elevated mercury (Hg) concentrations in fish from Amazonia have been associated with gold-mining, hydroelectric dams and deforestation but few studies consider the role of petroleum extraction. Hg levels were determined in fish samples collected in three river basins in Ecuador and Peru with contrasting petroleum exploitation and land-use characteristics. The non-migratory, piscivorous species, Hoplias malabaricus, was used as a bioindicator. The rate of Hg increase with body weight for this species was significantly higher on the Corrientes River, near the site of a recent oil spill, than on the other two rivers. In the absence of substantial deforestation and other anthropogenic sources in the Corrientes River basin, this finding suggests that oil contamination in Andean Amazonia may have a significant impact on Hg levels in fish.

  19. The effects of governance modes on the energy matrix of Andean countries

    International Nuclear Information System (INIS)

    Fontaine, Guillaume

    2011-01-01

    This article addresses the consequences of different modes of energy governance on the energy matrix. Energy governance is understood as a regulation system of the energy related interplays between the State, the society and the economy. The energy matrix is a useful instrument for comparative policy analysis, since it informs us about production and consumption trends, by sources and sectors. Our central argument is that energy governance follows two different patterns, one hierarchical and the other cooperative, that are not necessarily determined by the initial factors allocation, and produce different effects on the energy matrix. Hierarchical governance is based on centralized decision-making and State-centered development, while co-governance is based on decentralized decision-making and market-oriented development. To develop this argument, we compare the energy matrix from the five Andean countries (Venezuela, Colombia, Ecuador, Peru and Bolivia).

  20. The effects of governance modes on the energy matrix of Andean countries

    Energy Technology Data Exchange (ETDEWEB)

    Fontaine, Guillaume, E-mail: gfontaine@flacso.org.e [Latin American Faculty for Social Sciences (FLACSO), Research Laboratory on Governance, Quito (Ecuador)

    2011-05-15

    This article addresses the consequences of different modes of energy governance on the energy matrix. Energy governance is understood as a regulation system of the energy related interplays between the State, the society and the economy. The energy matrix is a useful instrument for comparative policy analysis, since it informs us about production and consumption trends, by sources and sectors. Our central argument is that energy governance follows two different patterns, one hierarchical and the other cooperative, that are not necessarily determined by the initial factors allocation, and produce different effects on the energy matrix. Hierarchical governance is based on centralized decision-making and State-centered development, while co-governance is based on decentralized decision-making and market-oriented development. To develop this argument, we compare the energy matrix from the five Andean countries (Venezuela, Colombia, Ecuador, Peru and Bolivia).

  1. International comparison of resistance thermometers between NMIs from Spain, Mexico and Andean countries

    Science.gov (United States)

    del Campo, D.; Ruiz, V. C.; Méndez-Lango, E.; Córdova, L.; von Borries, E.; Sánchez, C. A.; Arévalo, A.; Aguilera, B.; Guillén, E.; Cabrera, C.; Quintana, L.

    2013-09-01

    An international comparison on semi-standard Platinum Resistance Thermometers (PRTs) among the National Metrology Institutes (NMIs) of Spain, Mexico and the Andean Countries (Bolivia, Colombia, Ecuador, Peru and Venezuela) began in 2004 and was successfully completed in 2005. Two PRTs were circulated (hand carried) and compared from -40 °C up to 250 °C. The Centro Español de Metrología (Spanish NMI), CEM, was the pilot laboratory and the Centro Nacional de Metrología (Mexican NMI), CENAM, was the co-pilot laboratory. This paper shows the details of the comparison and the final results as they were presented in the approved final report of the comparison in September of 2005.

  2. Genome-Wide Association Study of Anthracnose Resistance in Andean Beans (Phaseolus vulgaris.

    Directory of Open Access Journals (Sweden)

    Grady H Zuiderveen

    Full Text Available Anthracnose is a seed-borne disease of common bean (Phaseolus vulgaris L. caused by the fungus Colletotrichum lindemuthianum, and the pathogen is cosmopolitan in distribution. The objectives of this study were to identify new sources of anthracnose resistance in a diverse panel of 230 Andean beans comprised of multiple seed types and market classes from the Americas, Africa, and Europe, and explore the genetic basis of this resistance using genome-wide association mapping analysis (GWAS. Twenty-eight of the 230 lines tested were resistant to six out of the eight races screened, but only one cultivar Uyole98 was resistant to all eight races (7, 39, 55, 65, 73, 109, 2047, and 3481 included in the study. Outputs from the GWAS indicated major quantitative trait loci (QTL for resistance on chromosomes, Pv01, Pv02, and Pv04 and two minor QTL on Pv10 and Pv11. Candidate genes associated with the significant SNPs were detected on all five chromosomes. An independent QTL study was conducted to confirm the physical location of the Co-1 locus identified on Pv01 in an F4:6 recombinant inbred line (RIL population. Resistance was determined to be conditioned by the single dominant gene Co-1 that mapped between 50.16 and 50.30 Mb on Pv01, and an InDel marker (NDSU_IND_1_50.2219 tightly linked to the gene was developed. The information reported will provide breeders with new and diverse sources of resistance and genomic regions to target in the development of anthracnose resistance in Andean beans.

  3. Seismic imaging of the upper mantle beneath the northern Central Andean Plateau: Implications for surface topography

    Science.gov (United States)

    Ward, K. M.; Zandt, G.; Beck, S. L.; Wagner, L. S.

    2015-12-01

    Extending over 1,800 km along the active South American Cordilleran margin, the Central Andean Plateau (CAP) as defined by the 3 km elevation contour is second only to the Tibetan Plateau in geographic extent. The uplift history of the 4 km high Plateau remains uncertain with paleoelevation studies along the CAP suggesting a complex, non-uniform uplift history. As part of the Central Andean Uplift and the Geodynamics of High Topography (CAUGHT) project, we use surface waves measured from ambient noise and two-plane wave tomography to image the S-wave velocity structure of the crust and upper mantle to investigate the upper mantle component of plateau uplift. We observe three main features in our S-wave velocity model including (1), a high velocity slab (2), a low velocity anomaly above the slab where the slab changes dip from near horizontal to a normal dip, and (3), a high-velocity feature in the mantle above the slab that extends along the length of the Altiplano from the base of the Moho to a depth of ~120 km with the highest velocities observed under Lake Titicaca. A strong spatial correlation exists between the lateral extent of this high-velocity feature beneath the Altiplano and the lower elevations of the Altiplano basin suggesting a potential relationship. Non-uniqueness in our seismic models preclude uniquely constraining this feature as an uppermost mantle feature bellow the Moho or as a connected eastward dipping feature extending up to 300 km in the mantle as seen in deeper mantle tomography studies. Determining if the high velocity feature represents a small lithospheric root or a delaminating lithospheric root extending ~300 km into the mantle requires more integration of observations, but either interpretation shows a strong geodynamic connection with the uppermost mantle and the current topography of the northern CAP.

  4. Inorganic mercury (Hg2+ uptake by different plankton fractions of Andean Patagonian lakes (Argentina

    Directory of Open Access Journals (Sweden)

    Diéguez M.C.

    2014-07-01

    Full Text Available The species composition and the size structure of natural planktonic food webs may provide essential information to understand the fate of mercury and, in particular, the bioaccumulation pattern of Hg2+ in the water column of lake ecosystems. Heterotrophic and autotrophic picoplankton and phytoplankton are the most important entry points for Hg in aquatic ecosystems since they concentrate Hg2+ and MeHg from ambient water, making them available to planktonic consumers at higher trophic levels of lake food webs. In this investigation we studied the uptake of 197Hg2+ in natural plankton assemblages from four Andean lakes (Nahuel Huapi National Park, Patagonia, Argentina, comprised in the size fractions 0.2-2.7 μm (picoplankton, 0.2-20 μm (pico and nanoplankton and 20-50 μm (microplankton through experiments using Hg2+ labeled with 197Hg2+. The experimental results showed that the uptake of Hg2+ was highest in the smallest plankton fractions (0.2-2.7 μm and 0.2-20 μm compared to the larger fraction comprising microplankton (20-50 um. This pattern was consistent in all lakes, reinforcing the idea that among pelagic organisms, heterotrophic and autotrophic bacteria with the contribution of nanoflagellates and dinoflagellates constitute the main entry point of Hg2+ to the pelagic food web. Moreover, a significant direct relationship was found between the Hg2+ uptake and surface index of the planktonic fractions (SIf. Thus, the smaller planktonic fractions which bore the higher SI were the major contributors to the Hg2+ passing from the abiotic to the biotic pelagic compartments of these Andean lakes.

  5. TREATMENT SUCCESS IN THREE ANDEAN BEARS (TREMARCTOS ORNATUS) WITH ALOPECIA SYNDROME USING OCLACITINIB MALEATE (APOQUEL®).

    Science.gov (United States)

    Drake, Gabby J; Nuttall, Tim; López, Javier; Magnone, William; Leclerc, Antoine; Potier, Romain; Lécu, Alexis; Guézénec, Maëlle; Kolter, Lydia; Nicolau, Amélie; Lemberger, Karin; Pin, Didier; Cosgrove, Sallie B

    2017-09-01

    Andean bear (Tremarctos ornatus) alopecia syndrome (ABAS) commonly affects captive bears, particularly sexually mature females. ABAS is characterized by bilaterally symmetrical predominantly flank alopecia with or without profound pruritus and secondary bacterial and Malassezia infections. There is no effective treatment and severely affected bears have been euthanized. This paper describes the successful management of ABAS in three female Andean bears. Skin biopsies and cytology revealed a mixed dermal inflammatory infiltrate, alopecia, hyperkeratosis, and Malassezia dermatitis. Allergen specific serology was positive for environmental allergens in one case. Hematology, serum biochemistry, and thyroid and adrenal function were normal in all cases. There was no consistent response to novel diet trials, antifungals, antihistamines, allergen specific immunotherapy, or topical antimicrobials. There was a partial response to ciclosporin (Atopica® cat, Novartis Animal Health; 5 mg/kg po, sid) in one case and oral glucocorticoids in all cases (dexamethasone sodium phosphate, [Colvasone 0.2%, Norbrook], 0.15 mg/kg po, sid or prednisolone [Deltacortene, Bruno Farmaceutici, and Megasolone 20, Coophavet], 0.3-1.2 mg/kg po, sid), but treatment was withdrawn following adverse effects. Treatment with oclacitinib maleate (Apoquel®, Zoetis; 0.46-0.5 mg/kg po, bid) resulted in rapid and complete resolution of the pruritus with subsequent improvement in demeanor and fur regrowth. After 5 mo, the bears were almost fully furred and off all other medication. Treatment was tapered to the lowest dose that prevented relapse of the pruritus (0.23-0.4 mg/kg po, sid). No adverse effects have been noted. ABAS is usually an intractable condition, and, to our knowledge, oclacitinib is the first treatment shown to result in sustained clinical improvement. Further studies on the etiology of ABAS, and on efficacy and long-term safety of oclacitinib are needed.

  6. Phytosynthesis and photocatalytic activity of magnetite (Fe_3O_4) nanoparticles using the Andean blackberry leaf

    International Nuclear Information System (INIS)

    Kumar, Brajesh; Smita, Kumari; Cumbal, Luis; Debut, Alexis; Galeas, Salome; Guerrero, Victor H.

    2016-01-01

    In the present study, a simple, low cost, and ecofriendly synthesis of magnetite nanoparticles (Fe_3O_4 NPs) has been developed using Andean blackberry leaf extract. UV–vis spectroscopy technique were used to study the initial formation of Fe_3O_4 NPs. Morphology, crystallinity and surface properties of nanoparticles were studied using transmission electron microscopy (TEM), Dynamic light scattering (DLS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and Thermal gravimetric (TG) techniques. TEM and DLS characterization indicated the formation of spherical Fe_3O_4 NPs of average size 54.5 ± 24.6 nm. XRD and FTIR studies confirmed the existence of the cubic spinel phase of Fe_3O_4 NPs and Fe−O peak at 570 cm"−"1, whereas TG analysis indicated that the nanoparticles contain 94% metal and 6% capping ligand. It has been observed that, as-synthesized Fe_3O_4 NPs exhibited photocatalytic activity for degradation of organic dyes such as methylene blue (k = 0.0105475 min"−"1), congo red (k = 0.0043240 min"−"1), and methyl orange (k = 0.0028930 min"−"1), efficiently. The antioxidant activity of Fe_3O_4 NPs against 1, 1-diphenyl-2-picrylhydrazyl were also evaluated. - Highlights: • We report extracellular phytosynthesis of Fe_3O_4 nanoparticles using the Andean blackberry leaf. • The synthesized Fe_3O_4 nanoparticles are spherical and average size is 54.5 ± 24.6 nm. • It showed enhanced photocatalytic activity and weak antioxidant efficacy. • Environmentally benign, non-toxic and cost-effective method is suggested.

  7. Acceleration and novelty: community restoration speeds recovery and transforms species composition in Andean cloud forest.

    Science.gov (United States)

    Wilson, Sarah Jane; Rhemtulla, Jeanine M

    2016-01-01

    Community-based tropical forest restoration projects, often promoted as a win-win solution for local communities and the environment, have increased dramatically in number in the past decade. Many such projects are underway in Andean cloud forests, which, given their extremely high biodiversity and history of extensive clearing, are understudied. This study investigates the efficacy of community-based tree-planting projects to accelerate cloud forest recovery, as compared to unassisted natural regeneration. This study takes place in northwest Andean Ecuador, where the majority of the original, highly diverse cloud forests have been cleared, in five communities that initiated tree-planting projects to restore forests in 2003. In 2011, we identified tree species along transects in planted forests (n = 5), naturally regenerating forests (n = 5), and primary forests (n = 5). We also surveyed 120 households about their restoration methods, tree preferences, and forest uses. We found that tree diversity was higher in planted than in unplanted secondary forest, but both were less diverse than primary forests. Ordination analysis showed that all three forests had distinct species compositions, although planted forests shared more species with primary forests than did unplanted forests. Planted forests also contained more animal-dispersed species in both the planted canopy and in the unplanted, regenerating understory than unplanted forests, and contained the highest proportion of species with use value for local people. While restoring forest increased biodiversity and accelerated forest recovery, restored forests may also represent novel ecosystems that are distinct from the region's previous ecosystems and, given their usefulness to people, are likely to be more common in the future.

  8. Genome-Wide Association Study of Anthracnose Resistance in Andean Beans (Phaseolus vulgaris).

    Science.gov (United States)

    Zuiderveen, Grady H; Padder, Bilal A; Kamfwa, Kelvin; Song, Qijian; Kelly, James D

    2016-01-01

    Anthracnose is a seed-borne disease of common bean (Phaseolus vulgaris L.) caused by the fungus Colletotrichum lindemuthianum, and the pathogen is cosmopolitan in distribution. The objectives of this study were to identify new sources of anthracnose resistance in a diverse panel of 230 Andean beans comprised of multiple seed types and market classes from the Americas, Africa, and Europe, and explore the genetic basis of this resistance using genome-wide association mapping analysis (GWAS). Twenty-eight of the 230 lines tested were resistant to six out of the eight races screened, but only one cultivar Uyole98 was resistant to all eight races (7, 39, 55, 65, 73, 109, 2047, and 3481) included in the study. Outputs from the GWAS indicated major quantitative trait loci (QTL) for resistance on chromosomes, Pv01, Pv02, and Pv04 and two minor QTL on Pv10 and Pv11. Candidate genes associated with the significant SNPs were detected on all five chromosomes. An independent QTL study was conducted to confirm the physical location of the Co-1 locus identified on Pv01 in an F4:6 recombinant inbred line (RIL) population. Resistance was determined to be conditioned by the single dominant gene Co-1 that mapped between 50.16 and 50.30 Mb on Pv01, and an InDel marker (NDSU_IND_1_50.2219) tightly linked to the gene was developed. The information reported will provide breeders with new and diverse sources of resistance and genomic regions to target in the development of anthracnose resistance in Andean beans.

  9. Neurocognitive screening of lead-exposed andean adolescents and young adults.

    Science.gov (United States)

    Counter, S Allen; Buchanan, Leo H; Ortega, Fernando

    2009-01-01

    This study was designed to assess the utility of two psychometric tests with putative minimal cultural bias for use in field screening of lead (Pb)-exposed Ecuadorian Andean workers. Specifically, the study evaluated the effectiveness in Pb-exposed adolescents and young adults of a nonverbal reasoning test standardized for younger children, and compared the findings with performance on a test of auditory memory. The Raven Coloured Progressive Matrices (RCPM) was used as a test of nonverbal intelligence, and the Digit Span subtest of the Wechsler IV intelligence scale was used to assess auditory memory/attention. The participants were 35 chronically Pb-exposed Pb-glazing workers, aged 12-21 yr. Blood lead (PbB) levels for the study group ranged from 3 to 86 microg/dl, with 65.7% of the group at and above 10 microg/dl. Zinc protoporphyrin heme ratios (ZPP/heme) ranged from 38 to 380 micromol/mol, with 57.1% of the participants showing abnormal ZPP/heme (>69 micromol/mol). ZPP/heme was significantly correlated with PbB levels, suggesting chronic Pb exposure. Performance on the RCPM was less than average on the U.S., British, and Puerto Rican norms, but average on the Peruvian norms. Significant inverse associations between PbB/ZPP concentrations and RCPM standard scores using the U.S., Puerto Rican, and Peruvian norms were observed, indicating decreasing RCPM test performance with increasing PbB and ZPP levels. RCPM scores were significantly correlated with performance on the Digit Span test for auditory memory. Mean Digit Span scale score was less than average, suggesting auditory memory/attention deficits. In conclusion, both the RCPM and Digit Span tests were found to be effective instruments for field screening of visual-spatial reasoning and auditory memory abilities, respectively, in Pb-exposed Andean adolescents and young adults.

  10. Diversification rates, host plant shifts and an updated molecular phylogeny of Andean Eois moths (Lepidoptera: Geometridae.

    Directory of Open Access Journals (Sweden)

    Patrick Strutzenberger

    Full Text Available Eois is one of the best-investigated genera of tropical moths. Its close association with Piper plants has inspired numerous studies on life histories, phylogeny and evolutionary biology. This study provides an updated view on phylogeny, host plant use and temporal patterns of speciation in Eois. Using sequence data (2776 bp from one mitochondrial (COI and one nuclear gene (Ef1-alpha for 221 Eois species, we confirm and reinforce previous findings regarding temporal patterns of diversification. Deep diversification within Andean Eois took place in the Miocene followed by a sustained high rate of diversification until the Pleistocene when a pronounced slowdown of speciation is evident. In South America, Eois diversification is very likely to be primarily driven by the Andean uplift which occurred concurrently with the entire evolutionary history of Eois. A massively expanded dataset enabled an in-depth look into the phylogenetic signal contained in host plant usage. This revealed several independent shifts from Piper to other host plant genera and families. Seven shifts to Peperomia, the sister genus of Piper were detected, indicating that the shift to Peperomia was an easy one compared to the singular shifts to the Chloranthaceae, Siparunaceae and the Piperacean genus Manekia. The potential for close co-evolution of Eois with Piper host plants is therefore bound to be limited to smaller subsets within Neotropical Eois instead of a frequently proposed genus-wide co-evolutionary scenario. In regards to Eois systematics we confirm the monophyly of Neotropical Eois in relation to their Old World counterparts. A tentative biogeographical hypothesis is presented suggesting that Eois originated in tropical Asia and subsequently colonized the Neotropics and Africa. Within Neotropical Eois we were able to identify the existence of six clades not recognized in previous studies and confirm and reinforce the monophyly of all 9 previously delimited

  11. The role of collisional tectonics in the metallogeny of the Central Andean tin belt [rapid communication

    Science.gov (United States)

    Mlynarczyk, Michael S. J.; Williams-Jones, Anthony E.

    2005-12-01

    The Inner Arc of the Central Andes, broadly corresponding to the Eastern Cordillera, is the location of a rich Tertiary and Triassic Sn-W-(Ag-base metal) metallogenic province, commonly referred to as the Bolivian tin belt. We propose that the Tertiary metallogeny, which generated most of the tin ores, was a direct consequence of discrete "collisions" between the South American plate and the Nazca slab and sub-slab mantle, during the ongoing Andean orogeny. Evidence supporting this proposal include: (1) the coincidence of the tin province and the Inner Arc in a marked "hump" in the Andean orogen, which may represent tectonic indentation; (2) the symmetry of the tin province with respect to the Bolivian orocline, the axis of which corresponds to the direction of highest compression; (3) the relative symmetry of the magmatism and tin mineralization with respect to this axis; (4) the concurrent timing of mineralization and compressional pulses; (5) the similar host rock geochemistry and ore lead isotope data, testifying to a common crustal reservoir; and (6) the striking similarity of the igneous suites, associated with the ore deposits to those from "typical" collisional orogens. A number of studies have called upon a persistent tin anomaly to explain the metallogeny of the region. We propose, instead, that the latter is better explained by periodic compressional interaction between the Farallon/Nazca oceanic plate and the South American continent. This led to the generation of peraluminous magmas, which during fractional crystallization exsolved the fluids responsible for the voluminous Sn-W mineralization.

  12. Uptake of Hg2+ by picocyanobacteria in natural water from four Andean lakes

    Directory of Open Access Journals (Sweden)

    Diéguez M.C.

    2014-07-01

    Full Text Available In lake food webs, planktonic bacteria and algae represent the greatest bioconcentration step for Hg2+ and monomethyl-Hg (MeHg. As they are the most abundant organisms in planktonic trophic webs and also the main food resource for herbivorous plankton, they can mobilize large amounts of Hg to higher trophic levels. In Andean Patagonian lakes (Argentina, dissolved organic matter (DOM concentration and character, coupled with photo-reactions, play a central role in the complexation of Hg2+ in the water column and can even regulate the uptake of Hg2+ by planktonic algae. In this investigation we evaluated the DOM character of natural waters (NW from four Andean lakes and studied its influence on the uptake of 197Hg2+ in a strain of the picocyanobacteria Synechococcus by using Hg2+ labeled with 197Hg2+. The uptake of radiolabeled Hg2+ by Synechococcus showed different magnitude in NW of lakes Moreno, El Trébol, Morenito and Escondido. Increasing lake DOM concentration reduced the bioavailability of Hg2+ as indicated by the lower uptakes rates found in NW with higher complexity and concentration of the DOM pool. Uptakes of Hg2+ by this picocyanobacteria contrasted among NW from pelagic (surface and bottom and littoral compartments of Lake Escondido which suggest that the entry of this metal may be highly variable even in the same environment. The study of the uptake of radiolabeled Hg2+ in a set of dilutions of NW from Lake Escondido demonstrated that the bioavailability of Hg2+ decrease with increasing DOM concentration.

  13. Effects of Flat Slab Subduction on Andean Thrust Kinematics and Foreland Basin Evolution in Western Argentina

    Science.gov (United States)

    Horton, B. K.; Fuentes, F.; McKenzie, N. R.; Constenius, K. N.; Alvarado, P. M.

    2014-12-01

    Debate persists over the effects of flat-slab subduction on the kinematics of overriding plate deformation and the evolution of retroarc sedimentary basins. In western Argentina, major spatial and temporal variations in the geometry of the subducting Nazca slab since ~15 Ma provide opportunities to evaluate the late Cenozoic response of the Andean fold-thrust belt and foreland basin to subhorizontal subduction. Preliminary results from several structural and sedimentary transects spanning the frontal thrust belt and foreland basin system between 31°S and 35°S reveal Oligocene-middle Miocene hinterland exhumation during normal-slab subduction followed thereafter by progressive slab shallowing with initial rapid cratonward propagation of ramp-flat thrust structures (prior to basement-involved foreland uplifts) and accompanying wholesale exhumation and recycling of the early Andean foreland basin (rather than regional dynamic subsidence). Detrital zircon U-Pb geochronologic data prove instrumental for revealing shifts in thrust-belt exhumation, defining depositional ages within the foreland basin, and constraining the timing of activity along frontal thrust structures. In both the San Juan (31-32°S) and Malargüe (34-35°S) segments of the fold-thrust belt, geochronological results for volcaniclastic sandstones and syndeformational growth strata are consistent with a major eastward advance in shortening at 12-9 Ma. This episode of rapid thrust propagation precedes the reported timing of Sierras Pampeanas basement-involved foreland uplifts and encompasses modern regions of both normal- and flat-slab subduction, suggesting that processes other than slab dip (such as inherited crustal architecture, critical wedge dynamics, and arc magmatism) are additional regulators of thrust-belt kinematics and foreland basin evolution.

  14. Thoracic skeletal morphology and high-altitude hypoxia in Andean prehistory.

    Science.gov (United States)

    Weinstein, Karen J

    2007-09-01

    Living humans from the highland Andes exhibit antero-posteriorly and medio-laterally enlarged chests in response to high-altitude hypoxia. This study hypothesizes that morphological responses to high-altitude hypoxia should also be evident in pre-Contact Andean groups. Thoracic skeletal morphology in four groups of human skeletons (N = 347) are compared: two groups from coastal regions (Ancón, Peru, n = 79 and Arica, Chile, n = 123) and two groups from high altitudes (San Pedro de Atacama, Chile, n = 102 and Machu Picchu and Cuzco, Peru, n = 43). Osteometric variables that represent proportions of chest width and depth include sternal and clavicular lengths and breadths and rib length, curvature, and area. Each variable was measured relative to body size, transformed into logarithmic indices, and compared across sex-specific groups using ANOVA and Tukey multiple comparison tests. Atacama highlanders have the largest sternal and clavicular proportions and ribs with the greatest area and least amount of curvature, features that suggest an antero-posteriorly deep and mediolaterally wide thoracic skeleton. Ancón lowlanders exhibit proportions indicating narrower and shallower chests. Machu Picchu and Cuzco males cluster with the other highland group in rib curvature and area at the superior levels of the thorax, whereas chest proportions in Machu Picchu and Cuzco females resemble those of lowlanders. The variation in Machu Picchu and Cuzco males and females is interpreted as the result of population migrations. The presence of morphological traits indicative of enlarged chests in some highland individuals suggests that high-altitude hypoxia was an environmental stressor shaping the biology of highland Andean groups during the pre-Contact period. (c) 2007 Wiley-Liss, Inc.

  15. Energy, water vapor and carbon fluxes in Andean agroecosystems: conceptualization and methodological standardization

    Directory of Open Access Journals (Sweden)

    Angela María Castaño Marín

    2017-01-01

    Full Text Available This paper presents the conceptualization, methodological adjustment and experimental application of the micrometeorological technique eddy covariance - EC, to measure energy, water vapor and CO2 fluxes in two coffee agroecosystems: the first under full sunlight, and the second under shade, both with equatorial Andean hillslope conditions. With a footprint and fetch calculation, the required distance from the edge of the field in the prevailing wind direction to the EC tower is three times higher under shade than full sun. The shaded agroecosystem reached maximum average carbon fixation rates of 21.26 ± 2.469 μmolCO2.m-2s-1 ( = 0.05 (61% higher than under 100% sunlight which gives a high carbon sink capacity to the association of coffee plants with shading Pigeon peas (Cajanus cajan L. The average evapotranspiration rate was 2.33 ± 0.0102 mm.d-1 ( = 0.05 and 2.08 ± 0.00732 mm.d-1 under shade and 100% sunlight, respectively. The proportion of net radiation that reached the soil was 2% under shade and 4% under 100% sunlight. Likewise, the soil energy loss during the night was lower under shade, indicating less day-night temperature range in the latter agroecosystem. The methodological adjustment and the results of this first work using EC in Colombian coffee plantations, contribute to the development of reliable research regarding gas and energy exchanges between the atmosphere and ecosystems in conditions of the equatorial Andean hillslope.

  16. Accumulation rate in a tropical Andean glacier as a proxy for northern Amazon precipitation

    Science.gov (United States)

    da Rocha Ribeiro, Rafael; Simões, Jefferson Cardia; Ramirez, Edson; Taupin, Jean-Denis; Assayag, Elias; Dani, Norberto

    2018-04-01

    Andean tropical glaciers have shown a clear shrinkage throughout the last few decades. However, it is unclear how this general retreat is associated with variations in rainfall patterns in the Amazon basin. To investigate this question, we compared the annual net accumulation variations in the Bolivian Cordillera Real (Andes), which is derived from an ice core from the Nevado Illimani (16° 37' S, 67° 46' W), covering the period 1960-1999 using the Amazonian Rainfall Index, Northern Atlantic Index (TNA), Multivariate ENSO Index (MEI), and Pacific Decadal Oscillation (PDO). The accumulation rate at the Nevado Illimani ice core decreased by almost 25% after 1980, from 1.02 w.eq. a-1 (water equivalent per year) in the 1961-1981 period to 0.76 w.eq. a-1 in the 1981-1999 period. The Northern Amazonian Rainfall (NAR) index best reflects changes in accumulation rates in the Bolivian ice core. Our proposal is based on two observations: (1) This area shows reduced rainfall associated with a more frequent and intense El Niño (during the positive phase of the MEI). The opposite (more rain) is true during La Niña phases. (2) Comparisons of the ice core record and NAR, PDO, and MEI indexes showed similar trends for the early 1980s, represented by a decrease in the accumulation rates and its standard deviations, probably indicating the same causality. The general changes observed by early 1980s coincided with the beginning of a PDO warm phase. This was followed by an increase in the Amazonian and tropical Andean precipitation from 1999, coinciding with a new PDO phase. However, this increase did not result in an expansion of the Zongo Glacier area.

  17. Phlebotomine sandflies (Diptera: Psychodidae) in a sub-Andean forest from the Norte de Santander, Colombia.

    Science.gov (United States)

    Hoyos-López, Richard; Bolaños, Rafael; Contreras-Gutierrez, MarIa; Carrero-Sarmiento, Diego

    2016-03-01

    The recognition of communities of arthropods with medical importance in natural systems constitutes an important step in the prediction of possible epidemic events and/or emergence of infectious diseases in the human population. This is due to anthropogenic impact in natural areas and landscape modification, which changes the dynamics of pathogenic agents, reservoirs, and vector insects. In this study, an inventory was compiled of species of the genus Lutzomyia present in sub-Andean forest from the confluence of the Pamplonita River basin. CDC-light and Shannon traps were used for collecting adult phlebotomine sandflies during the month of October 2013 in a sub-Andean forest from river basin Pamplonita. All specimens were identified using morphological keys. The epidemiological relevance of each species was reported using a literature review about natural infection or vector incrimination with Leishmania species or other pathogens microorganism. A total of 2755 specimens belonging to eight species of the genus Lutzomyia were collected. Out of the eight species, seven belonged to the group verrucarum (Lutzomyia sp--townsendi series, L. ovallesi, L. spinicrassa, L. serrana, L. townsendi, L. nuneztovari and L. pia), while one belonged to the subgenus Helcocyrtomyia (L. hartmanni). A new registry of L. townsendi was observed for the Norte de Santander department. The appreciable diversity of the verrucarum group observed in this area suggest further investigation on the biogeography and evolution of this group, and epidemiological risk for human populations around this area, as there are reports of Leishmania natural infection and favourable conditions for domestication of phlebotomines in rural towns.

  18. A comparative study of nemertean complete mitochondrial genomes, including two new ones for Nectonemertes cf. mirabilis and Zygeupolia rubens, may elucidate the fundamental pattern for the phylum Nemertea

    Directory of Open Access Journals (Sweden)

    Chen Hai-Xia

    2012-04-01

    Full Text Available Abstract Background The mitochondrial genome is important for studying genome evolution as well as reconstructing the phylogeny of organisms. Complete mitochondrial genome sequences have been reported for more than 2200 metazoans, mainly vertebrates and arthropods. To date, from a total of about 1275 described nemertean species, only three complete and two partial mitochondrial DNA sequences from nemerteans have been published. Here, we report the entire mitochondrial genomes for two more nemertean species: Nectonemertes cf. mirabilis and Zygeupolia rubens. Results The sizes of the entire mitochondrial genomes are 15365 bp for N. cf. mirabilis and 15513 bp for Z. rubens. Each circular genome contains 37 genes and an AT-rich non-coding region, and overall nucleotide composition is AT-rich. In both species, there is significant strand asymmetry in the distribution of nucleotides, with the coding strand being richer in T than A and in G than C. The AT-rich non-coding regions of the two genomes have some repeat sequences and stem-loop structures, both of which may be associated with the initiation of replication or transcription. The 22 tRNAs show variable substitution patterns in nemerteans, with higher sequence conservation in genes located on the H strand. Gene arrangement of N. cf. mirabilis is identical to that of Paranemertes cf. peregrina, both of which are Hoplonemertea, while that of Z. rubens is the same as in Lineus viridis, both of which are Heteronemertea. Comparison of the gene arrangements and phylogenomic analysis based on concatenated nucleotide sequences of the 12 mitochondrial protein-coding genes revealed that species with closer relationships share more identical gene blocks. Conclusion The two new mitochondrial genomes share many features, including gene contents, with other known nemertean mitochondrial genomes. The tRNA families display a composite substitution pathway. Gene order comparison to the proposed ground pattern of

  19. Surname-inferred Andean ancestry is associated with child stature and limb lengths at high altitude in Peru, but not at sea level.

    Science.gov (United States)

    Pomeroy, Emma; Wells, Jonathan C K; Stanojevic, Sanja; Miranda, J Jaime; Moore, Lorna G; Cole, Tim J; Stock, Jay T

    2015-01-01

    Native Andean ancestry gives partial protection from reduced birthweight at high altitude in the Andes compared with European ancestry. Whether Andean ancestry is also associated with body proportions and greater postnatal body size at altitude is unknown. Therefore, we tested whether a greater proportion of Andean ancestry is associated with stature and body proportions among Peruvian children at high and low altitude. Height, head circumference, head-trunk height, upper and lower limb lengths, and tibia, ulna, hand and foot lengths, were measured in 133 highland and 169 lowland children aged 6 months to 8.5 years. For highland and lowland groups separately, age-sex-adjusted anthropometry z scores were regressed on the number of indigenous parental surnames as a proxy for Andean ancestry, adjusting for potential confounders (maternal age and education, parity, altitude [highlands only]). Among highland children, greater Andean ancestry was negatively associated with stature and tibia, ulna, and lower limb lengths, independent of negative associations with greater altitude for these measurements. Relationships were strongest for tibia length: each additional Andean surname or 1,000 m increase at altitude among highland children was associated with 0.18 and 0.65 z score decreases in tibia length, respectively. Anthropometry was not significantly associated with ancestry among lowland children. Greater Andean ancestry is associated with shorter stature and limb measurements at high but not low altitude. Gene-environment interactions between high altitude and Andean ancestry may exacerbate the trade-off between chest dimensions and stature that was proposed previously, though we could not test this directly. © 2015 Wiley Periodicals, Inc.

  20. Tropical Andean ecosystems and the need to keep warming limits below a +1.5°C threshold

    Science.gov (United States)

    Ruiz-Carrascal, D.; Herzog, S. K.; Guitierrez Lagoueyte, M. E.; Gonzalez-Duque, D.; Cuevas-Moreno, J.; del Valle, J. I.; Andreu-Hayles, L.; Herrera, D. A.; Martínez, R.

    2017-12-01

    Long-term climate change and rapid land-use change are synergistically threatening the integrity and functioning of tropical Andean ecosystems. The main goal of our research was to integrate climate change projections, biodiversity data and anthropogenically driven ecosystem disruption assessments to quantify the vulnerability of Andean ecosystems and species to global change at a local scale. We merged discernible trends in local quality-controlled weather station data with reanalysis data, as well as with historical and prospective simulation outputs of five well-known GCMs to assess a long-term context for the analysis of climate change exposure (temperature severity intervals). Individual, medium-term, multi-member GCM simulations included: altitude-corrected 2046-2065 (IPCC-AR4) climate change scenarios for the A1B emission scenario; and spatially-downscaled 2040-2069 (IPCC-AR5) projections for the RCP4.5. Previous studies reported mean annual temperature anomaly intervals that resulted in exceedingly high thresholds: the lowest severity interval ( +2.71°C). The least severe interval extended up to the threshold widely recognized as `dangerous' climate change, thereby leading to an underestimation of the true vulnerability of Andean species. Our analyses suggest that temperature anomalies for the full extent of the tropical Andes will likely range from low ( +2.61°C), exceeding the threshold of 'natural' climate variability (+1.78°C). Our results suggest that most species that were used as proxies of ecosystem vulnerabilities will likely experience overall low-to-medium-to-high temperature increases. Since many of them have potentially high sensitivity to such long-term changes, Andean species will likely experience greatly increases in vulnerability. The already-disrupted Andean ecosystems will suffer a further climatic stress, which will worsen the well-known detrimental synergies between climate and land-use changes. There is an imperative need to

  1. On the Relationship of Dynamic Forearc Processes in Southern Peru to the Development and Preservation of Andean Topography

    Science.gov (United States)

    Hall, S. R.; Farber, D. L.; Audin, L.; Saillard, M.; Finkel, R. C.

    2008-12-01

    After more than 40 years of study, the timing and nature of Andean uplift remains an area of great scientific debate. The forearc of the Andean margin is of particular neotectonic interest, as previous models of Andean orogenesis attributed little-no Neogene deformation to the western margin of Altiplano. However, using the combination of remote sensing with high-resolution data, in situ cosmogenic isotope concentrations and thermochronology, in recent years the community has made important advances in addressing the rates, timings, styles, and locations of active deformation within the forearc of the Andean margin. To first order, we find that - both in terms of tectonics and climate - since 10Ma, the Andean forearc has been quite a dynamic region. Neotectonic studies in this region have been facilitated by the high degree of geomorphic surface preservation that the hyperarid (for at least the last 3My) coastal Atacama Desert has provided. Specifically, in southern Peru (14°-18°S), vast pediment surfaces have been abandoned through incision along the major river drainages that carve the deep canyons into the Precordillera and Western Cordillera. While the exact timing of the periods of more intense incision plausibly correspond with climate events, the total amount of incision integrated over many climate cycles is a useful indicator of tectonic activity. In this region, we find a number of geomorphic and structural features that provide strong evidence for distributed crustal deformation along range-sub-parallel contractile and strike-slip structures. Specifically, we see 1) ancient surfaces reflecting erosion rates as low as chronologies, and 6) Pleistocene mass-wasting events accommodating the redistribution of ~109-1010 m3 of material per event. Furthermore, the observation that Pleistocene incision rates are comparable with Late Miocene and Pliocene rates, suggests to us, that the rates and style of surface uplift within the forearc of southern Peru has

  2. Biogas production from energy crops and crop residues

    Energy Technology Data Exchange (ETDEWEB)

    Lehtomaeki, A.

    2006-07-01

    The feasibility of utilising energy crops and crop residues in methane production through anaerobic digestion in boreal conditions was evaluated in this thesis. Potential boreal energy crops and crop residues were screened for their suitability for methane production, and the effects of harvest time and storage on the methane potential of crops was evaluated. Codigestion of energy crops and crop residues with cow manure, as well as digestion of energy crops alone in batch leach bed reactors with and without a second stage upflow anaerobic sludge blanket reactor (UASB) or methanogenic filter (MF) were evaluated. The methane potentials of crops, as determined in laboratory methane potential assays, varied from 0.17 to 0.49 m3 CH{sub 4} kg-1 VS{sub added} (volatile solids added) and from 25 to 260 m3 CH4 t-1 ww (tons of wet weight). Jerusalem artichoke, timothy-clover and reed canary grass gave the highest methane potentials of 2 900-5 400 m3 CH{sub 4} ha-1, corresponding to a gross energy potential of 28-53 MWh ha-1 and 40 000-60 000 km ha-1 in passenger car transport. The methane potentials per ww increased with most crops as the crops matured. Ensiling without additives resulted in minor losses (0-13%) in the methane potential of sugar beet tops but more substantial losses (17-39%) in the methane potential of grass, while ensiling with additives was shown to have potential in improving the methane potentials of these substrates by up to 19-22%. In semi-continuously fed laboratory continuously stirred tank reactors (CSTRs) co-digestion of manure and crops was shown feasible with feedstock VS containing up to 40% of crops. The highest specific methane yields of 0.268, 0.229 and 0.213 m3 CH{sub 4} kg-1 VS{sub added} in co-digestion of cow manure with grass, sugar beet tops and straw, respectively, were obtained with 30% of crop in the feedstock, corresponding to 85-105% of the methane potential in the substrates as determined by batch assays. Including 30% of crop in

  3. Stable Food Crops Turning Into Commercial Crops: Case studies of ...

    African Journals Online (AJOL)

    RahelYilma

    case study analyses for the cereal crops of teff3, wheat and rice. Specifically, the ... behavior of households during the process of commercial transformation of subsistence ..... roducer → rural assembler, and producer → consumer. As with teff ...

  4. Recycling crop residues for use in recirculating hydroponic crop production

    Science.gov (United States)

    Mackowiak, C. L.; Garland, J. L.; Sager, J. C.

    1996-01-01

    As part of bioregenerative life support feasibility testing by NASA, crop residues are being used to resupply elemental nutrients to recirculating hydroponic crop production systems. Methods for recovering nutrients from crop residues have evolved from water soaking (leaching) to rapid aerobic bioreactor processing. Leaching residues recovered the majority of elements but it also recovered significant amounts of soluble organics. The high organic content of leachates was detrimental to plant growth. Aerobic bioreactor processing reduced the organic content ten-fold, which reduced or eliminated phytotoxic effects. Wheat and potato production studies were successful using effluents from reactors having with 8- to 1-day retention times. Aerobic bioreactor effluents supplied at least half of the crops elemental mass needs in these studies. Descriptions of leachate and effluent mineral content, biomass productivity, microbial activity, and nutrient budgets for potato and wheat are presented.

  5. Presence and significance of Bacillus thuringiensis Cry proteins associated with the Andean weevil Premnotrypes vorax (Coleoptera: Curculionidae

    Directory of Open Access Journals (Sweden)

    SilvioAlejandro López-Pazos

    2009-12-01

    Full Text Available The Andean weevil Premnotrypes vorax represents an important cause of damage to Colombian potato crops. Due to the impact of this plague on the economy of the country, we searched for new alternatives for its biological control, based on the entomopathogenic bacteria Bacillus thuringiensis. A total of 300 B. thuringiensis strains obtained from potato plantations infested with P. vorax were analyzed through crystal morphology, SDS-PAGE, PCR and bioassays. We used site- directed mutagenesis to modify the Cry3Aa protein. Most of the B. thuringiensis isolates had a bipyramidal crystal morphology. SDS-PAGE analyses had seven strains groups with σ-endotoxins from 35 to 135 kDa. The genes cry 2 and cry 1 were significantly more frequent in the P. vorax habitat (PCR analyses. Three mutant toxins, 1 (D354E, 2 (R345A, ∆Y350, ∆Y351, and 3 (Q482A, S484A, R485A, were analyzed to assess their activity against P. vorax larvae. Toxicity was low, or absent, against P. vorax for isolates, wild type cry 3Aa and cry 3Aa mutants. The genetic characterization of the collection provides opportunities for the selection of strains to be tested in bioassays against other insect pests of agricultural importance, and for designing Cry proteins with improved insecticidal toxicity. Rev. Biol. Trop. 57 (4: 1235-1243. Epub 2009 December 01.El gorgojo andino Premnotrypes vorax es una causa importante de daño en los cultivos colombianos de este tubérculo. Debido al impacto que esta plaga tiene sobre la economía del país, nos interesamos en buscar alternativas nuevas para el control biológico de P. vorax, basadas en la bacteria entomopatógena Bacillus thuringiensis. Se recolectaron un total de 300 cepas de B. thuringiensis a partir de plantaciones de papa infestadas con P. vorax, las cuales fueron analizadas por medio de la morfología del cristal, SDS-PAGE, PCR y ensayos biológicos. La mayoría de los aislamientos de B. thuringiensis presentaron cristales

  6. Preliminary checklist of the vascular flora of the Sub-Andean forest at Cuchilla El Fara (Santander-Colombia)

    International Nuclear Information System (INIS)

    Medina, Ruth; Reina, Miriam; Herrera, Edna; Avila, Fabio Andres; Chaparro, Omar; Cortes B, Rocio

    2010-01-01

    A preliminary checklist of the vascular flora of the sub-Andean forest of the Cuchilla El Fara is present ed. El Fara is located at the Guantiva - La Rusia - Iguaque biological corridor, in the municipalities of Charala, Gambita, and Suaita (Santander-Colombia). Information on habit, local altitudinal range, and collection number is recorded for each species. A total of 409 species of vascular plants included in 226 genera, and 105 families were recorded. The families with the highest number of genera were Rubiaceae (18), Asteraceae (10), Melastomataceae (10), Orchidaceae (10), Euphorbiaceae (8), Arecaceae (7) and Fabaceae (7). At specific level, the best represented families were Rubiaceae (33), Melastomataceae (28), Lauraceae (21), Asteraceae (17), Araceae (17), Orchidaceae (17) and Gesneriaceae (15). The affinities of the flora with other Neotropical sub-Andean forests are discussed. Finally, species of all IUCN threat categories are highlighted so that the information presented here can make a contribution to restoration and conservation programs.

  7. Landscape genetics, historical isolation and cross-Andean gene flow in the wax palm, Ceroxylon echinulatum (Arecaceae)

    DEFF Research Database (Denmark)

    Trénel, P.; Hansen, Michael Møller; Nordmand, S.

    2008-01-01

    -Huancabamba zone (AHZ) of southern Ecuador/northern Peru, as indicated by observations on fruit morphology. Second, we assessed the timeframe of cross-Andean divergence, and third, we investigated the impact of contemporary and historical landscape features on observed spatio-genetic patterns. Individual-based...... Bayesian clustering (BC) identified a northeastern, southeastern, southwestern, and northwestern cluster, with areas of genetic discontinuity coinciding with the Andes and the Giron-Paute deflection. F-statistics derived from BC suggested an east-to-west dispersal history. Population-based analyses...... impact of climatic friction. Coalescent analysis revealed that cross-Andean divergence took place during the Quaternary. Significant historical isolation (RST > FST) was found in the southwestern population. The current study illustrates a joint effect of founder dynamics, divergence by distance...

  8. Improvement of the Antioxidant Properties and Postharvest Life of Three Exotic Andean Fruits by UV-C Treatment

    Directory of Open Access Journals (Sweden)

    María J. Andrade-Cuvi

    2017-01-01

    Full Text Available Three Andean fruits naranjilla (Solanum quitoense Lam., uvilla (Physalis peruviana L., and mortiño (Vaccinium floribundum Kunth were subjected to prestorage UV-C treatments (0, 8, or 12.5 kJ m−2 and evaluated weekly to select the most suitable dose for fruit quality maintenance during storage (21 days at 6°C. The highest dose retains quality through lower deterioration index for all three fruits and was selected to further analyze the effects on physicochemical and antioxidant properties during storage. UV-C exposure delayed softening in naranjilla and increased soluble solid content in uvilla. UV-C also improved the maintenance of antioxidant capacity (AC in mortiño and uvilla. Overall, results indicate that short prestorage UV-C exposure may be an effective nonchemical approach to supplement low temperature storage, maintain quality, and extend the postharvest life of Andean naranjilla, uvilla, and mortiño fruit.

  9. African Crop Science Journal: Submissions

    African Journals Online (AJOL)

    Particular attention should be paid to the study factors/treatments and their structure, design, ... The African Crop Science Journal uses the Harvard citation style. Only published articles (journals and proceedings) or books may be cited.

  10. Plant senescence and crop productivity

    DEFF Research Database (Denmark)

    Gregersen, Per L.; Culetic, Andrea; Boschian, Luca

    2013-01-01

    Senescence is a developmental process which in annual crop plants overlaps with the reproductive phase. Senescence might reduce crop yield when it is induced prematurely under adverse environmental conditions. This review covers the role of senescence for the productivity of crop plants....... With the aim to enhance productivity, a number of functional stay-green cultivars have been selected by conventional breeding, in particular of sorghum and maize. In many cases, a positive correlation between leaf area duration and yield has been observed, although in a number of other cases, stay...... plants, the expression of the IPT gene under control of senescence-associated promoters has been the most successful. The promoters employed for senescence-regulated expression contain cis-elements for binding of WRKY transcription factors and factors controlled by abscisic acid. In most crops...

  11. Comparative study of emission of pollutant gases in vehicle M1, using fuel of the Andean Community

    OpenAIRE

    Jaime Fernando Antamba Guasgua; Guillermo Gorky Reyes Campaña; Miguel Estuardo Granja Paredes

    2016-01-01

    The environmental pollution is a problematics that concerns all countries about the world as result of this pollution there take place the phenomena of climate change, greenhouse effect, acid rain, and diseases in people. To delimit the issues, there were selected the countries that integrate the Andean Community, the project goal is compare by means of static and dynamic tests the values of emission of pollutant gases, with the fuel that is distributed in each of the selected countries. The ...

  12. Stable isotope variations (δ18O and δD) in modern waters across the Andean Plateau

    Science.gov (United States)

    Bershaw, John; Saylor, Joel E.; Garzione, Carmala N.; Leier, Andrew; Sundell, Kurt E.

    2016-12-01

    Environmental parameters that influence the isotopic composition of meteoric water (δ18O and δD) are well characterized up the windward side of mountains, where orographic precipitation results in a predictable relationship between the isotopic composition of precipitation and elevation. The topographic and climatic evolution of the Andean Plateau and surrounding regions has been studied extensively by exploiting this relationship through the use of paleowater proxies. However, interpretation on the plateau itself is challenged by a poor understanding of processes that fractionate isotopes during vapor transport and rainout, and by the relative contribution of unique moisture sources. Here, we present an extensive dataset of modern surface water samples for the northern Andean Plateau and surrounding regions to elucidate patterns and causes of isotope fractionation in this continental environment. These data show a progressive increase in δ18O of stream water west of the Eastern Cordillera (∼1‰/70 km), almost identical to the rate observed across the Tibetan Plateau, attributed to a larger fraction of recycled water in precipitation and/or increased evaporative enrichment downwind. This may lead to underestimates of paleoelevation, particularly for sites deep into the rainshadow of the Eastern Cordilleran crest. That said, elevation is a primary control on the isotopic composition of surface waters across the entire Andean Plateau and its flanks when considering the most negative δ18O values, highlighting the need for sufficiently large datasets to distinguish minimally evaporated samples. There is a general increase in δ18O on the plateau from north to south, concomitant with an increase in aridity and decrease in convective moistening (amount effect). Lastly, stable isotope and seasonal precipitation patterns suggest easterlies provide the vast majority of moisture that falls as precipitation across the Andean Plateau and Western Cordillera, from Peru to

  13. Can Andean medicine coexist with biomedical healthcare? A comparison of two rural communities in Peru and Bolivia.

    Science.gov (United States)

    Mathez-Stiefel, Sarah-Lan; Vandebroek, Ina; Rist, Stephan

    2012-07-24

    It is commonly assumed that indigenous medical systems remain strong in developing countries because biomedicine is physically inaccessible or financially not affordable. This paper compares the health-seeking behavior of households from rural Andean communities at a Peruvian and a Bolivian study site. The main research question was whether the increased presence of biomedicine led to a displacement of Andean indigenous medical practices or to coexistence of the two healing traditions. Open-ended interviews and free listing exercises were conducted between June 2006 and December 2008 with 18 households at each study site. Qualitative identification of households' therapeutic strategies and use of remedies was carried out by means of content analysis of interview transcriptions and inductive interference. Furthermore, a quantitative assessment of the incidence of culture-bound illnesses in local ethnobiological inventories was performed. Our findings indicate that the health-seeking behavior of the Andean households in this study is independent of the degree of availability of biomedical facilities in terms of quality of services provided, physical accessibility, and financial affordability, except for specific practices such as childbirth. Preference for natural remedies over pharmaceuticals coexists with biomedical healthcare that is both accessible and affordable. Furthermore, our results show that greater access to biomedicine does not lead to less prevalence of Andean indigenous medical knowledge, as represented by the levels of knowledge about culture-bound illnesses. The take-home lesson for health policy-makers from this study is that the main obstacle to use of biomedicine in resource-poor rural areas might not be infrastructural or economic alone. Rather, it may lie in lack of sufficient recognition by biomedical practitioners of the value and importance of indigenous medical systems. We propose that the implementation of health care in indigenous

  14. Can Andean medicine coexist with biomedical healthcare? A comparison of two rural communities in Peru and Bolivia

    Directory of Open Access Journals (Sweden)

    Mathez-Stiefel Sarah-Lan

    2012-07-01

    Full Text Available Abstract Background It is commonly assumed that indigenous medical systems remain strong in developing countries because biomedicine is physically inaccessible or financially not affordable. This paper compares the health-seeking behavior of households from rural Andean communities at a Peruvian and a Bolivian study site. The main research question was whether the increased presence of biomedicine led to a displacement of Andean indigenous medical practices or to coexistence of the two healing traditions. Methodology Open-ended interviews and free listing exercises were conducted between June 2006 and December 2008 with 18 households at each study site. Qualitative identification of households’ therapeutic strategies and use of remedies was carried out by means of content analysis of interview transcriptions and inductive interference. Furthermore, a quantitative assessment of the incidence of culture-bound illnesses in local ethnobiological inventories was performed. Results Our findings indicate that the health-seeking behavior of the Andean households in this study is independent of the degree of availability of biomedical facilities in terms of quality of services provided, physical accessibility, and financial affordability, except for specific practices such as childbirth. Preference for natural remedies over pharmaceuticals coexists with biomedical healthcare that is both accessible and affordable. Furthermore, our results show that greater access to biomedicine does not lead to less prevalence of Andean indigenous medical knowledge, as represented by the levels of knowledge about culture-bound illnesses. Conclusions The take-home lesson for health policy-makers from this study is that the main obstacle to use of biomedicine in resource-poor rural areas might not be infrastructural or economic alone. Rather, it may lie in lack of sufficient recognition by biomedical practitioners of the value and importance of indigenous medical systems

  15. Bayesian inferences suggest that Amazon Yunga Natives diverged from Andeans less than 5000 ybp: implications for South American prehistory.

    Science.gov (United States)

    Scliar, Marilia O; Gouveia, Mateus H; Benazzo, Andrea; Ghirotto, Silvia; Fagundes, Nelson J R; Leal, Thiago P; Magalhães, Wagner C S; Pereira, Latife; Rodrigues, Maira R; Soares-Souza, Giordano B; Cabrera, Lilia; Berg, Douglas E; Gilman, Robert H; Bertorelle, Giorgio; Tarazona-Santos, Eduardo

    2014-09-30

    Archaeology reports millenary cultural contacts between Peruvian Coast-Andes and the Amazon Yunga, a rainforest transitional region between Andes and Lower Amazonia. To clarify the relationships between cultural and biological evolution of these populations, in particular between Amazon Yungas and Andeans, we used DNA-sequence data, a model-based Bayesian approach and several statistical validations to infer a set of demographic parameters. We found that the genetic diversity of the Shimaa (an Amazon Yunga population) is a subset of that of Quechuas from Central-Andes. Using the Isolation-with-Migration population genetics model, we inferred that the Shimaa ancestors were a small subgroup that split less than 5300 years ago (after the development of complex societies) from an ancestral Andean population. After the split, the most plausible scenario compatible with our results is that the ancestors of Shimaas moved toward the Peruvian Amazon Yunga and incorporated the culture and language of some of their neighbors, but not a substantial amount of their genes. We validated our results using Approximate Bayesian Computations, posterior predictive tests and the analysis of pseudo-observed datasets. We presented a case study in which model-based Bayesian approaches, combined with necessary statistical validations, shed light into the prehistoric demographic relationship between Andeans and a population from the Amazon Yunga. Our results offer a testable model for the peopling of this large transitional environmental region between the Andes and the Lower Amazonia. However, studies on larger samples and involving more populations of these regions are necessary to confirm if the predominant Andean biological origin of the Shimaas is the rule, and not the exception.

  16. [Neurological paleopathology in the pre-Columbian cultures of the coast and the Andean plateau (I). Artificial cranial deformation].

    Science.gov (United States)

    Carod Artal, F J; Vázquez Cabrera, C B

    The aim of this work was to study the cranial trepanations and deformations carried out by the ancient Paraca, Huari, Tiahuanaco and Inca cultures. To do so, we conducted a field study involving visits to archaeological remains and anthropological museums on the Andean plateau and the Peruvian coast. Cranial deformation was more common in the Andean regions and was performed by putting little pieces of wood or compressive bandages on newborn infants' heads in order to modify the growth axis of the cranial cavity. Cranial deformations were performed for aesthetic and magic religious reasons, but were also used as a means of ethnic or social identification, as a symbol of nobility or to distinguish the ruling classes. The immediate consequence of such deformation was the modification of the normal process by which the cranial sutures close. There is a significant correlation between the presence of posterior and lateral wormian bones, according to the degree of artificial deformation. The persistence of metopic suture and exostosis of the outer ear canal have been found in 5% of the skulls belonging to pre Columbine mummies. Other paleopathological findings include cranial fractures (7%), porotic hyperostosis (25% of children's skulls), spina bifida occulta, signs of spinal disk arthrosis and Pott's disease. Artificial cranial deformation was a very widespread practice in the Andean regions in pre Columbine times.

  17. Taxonomic review of the species of Helina R.-D. (Diptera: Muscidae) from Andean-Patagonian forests.

    Science.gov (United States)

    Patitucci, Luciano Damián; Mulieri, Pablo Ricardo; Mariluis, Juan Carlos

    2016-08-12

    Helina Robineau-Desvoidy, 1830 is the second genus of Muscidae in terms of richness. This genus includes several species collected at high altitudes and high latitudes, and is poorly studied in the Neotropical region. Only 12 species of Helina have been recorded in the southern limit of South America in the Andean-Patagonian forests. In the present work, we studied all the species known from the Andean-Patagonian forests, with the exception of H. viola Malloch, 1934, present three new species, H. araucana sp. nov., H. dorada sp. nov., and H. ouina sp. nov., and provide the first description of the females of H. australis Carvalho & Pont, 1993 and H. rufoapicata Malloch, 1934. We also propose four new synonymies: H. nigrimana basilaris (Carvalho & Pont, 1993) and H. nigrimana grisea (Malloch, 1934) as new junior synonyms of H. nigrimana (Macquart, 1851); and H. fulvocalyptrata Malloch, 1934 and H. simplex Malloch, 1934 as new junior synonyms of H. chilensis Malloch, 1934. Finally, we provide a generic diagnosis and a new key for the Helina species of the Andean-Patagonian forests, as well as notes on the biology and distribution maps of each specimen, and discuss a preliminary contruction of groups of species.

  18. Crop diversity for yield increase.

    Directory of Open Access Journals (Sweden)

    Chengyun Li

    2009-11-01

    Full Text Available Traditional farming practices suggest that cultivation of a mixture of crop species in the same field through temporal and spatial management may be advantageous in boosting yields and preventing disease, but evidence from large-scale field testing is limited. Increasing crop diversity through intercropping addresses the problem of increasing land utilization and crop productivity. In collaboration with farmers and extension personnel, we tested intercropping of tobacco, maize, sugarcane, potato, wheat and broad bean--either by relay cropping or by mixing crop species based on differences in their heights, and practiced these patterns on 15,302 hectares in ten counties in Yunnan Province, China. The results of observation plots within these areas showed that some combinations increased crop yields for the same season between 33.2 and 84.7% and reached a land equivalent ratio (LER of between 1.31 and 1.84. This approach can be easily applied in developing countries, which is crucial in face of dwindling arable land and increasing food demand.

  19. Insights into Andean metallogenesis from the perspective of Re-Os analyses of sulfides

    International Nuclear Information System (INIS)

    Mathur, R.; Ruiz, J.R; Munizaga, F.M

    2001-01-01

    The source of metals and duration of mineralization for ore deposits are basic issues used to describe the metallogenesis of an area. In order to address these fundamental issues in the Andean Cordillera, we chose to analyze Re-Os isotopes in sulfides from twelve porphyry copper ore deposits that are spatially and temporally separated. Re-Os concentrates in sulfide minerals, and can thus provide genetic information on the source of metals and the timing of mineralization. The advantage of using Re-Os isotopes is that genetic and geochronologic information is obtained directly from the sulfide ore mineral, rather than by inferring chemical relationships from possibly unrelated alteration silicates. In this study we use the source of Os in low concentration sulfides as a proxy for the source of base metals. Initial ratios greater than the chondritic mantle (∼0.13) serve as indication of a crustal source for Os and, by inference, other ore forming metals. Molybdenite contains orders of magnitude more Re than the common sulfides and effectively no initial radiogenic 187 Os (Luck and Allegre, 1982). Therefore, it can be used as a high-precision geochronometer (McCandless, 1994; Stein et al. 1997). Re-Os data from low concentration sulfides and chronologic data from high concentration molybdenites highlight important processes within and among these Andean deposits.The low concentration sulfides contain between 5-30 ppt Os and 1- 30 ppb Re. There are two samples of hydrothermal and magmatic magnetite from Escondida that contain slightly more Re and Os than the other sulfides reported. The molybdenites have 187 Os concentrations that range from 5-2000 ppb, and Re concentrations that range from 60-3600 ppm. Mathur et al. (2000) and Mathur (2000) highlighted a strong relationship between the copper tonnage in Andean ore deposits and the Os initial ratio of sulfide minerals. The larger deposits have sulfide minerals with lower initial Os ratios than the smaller, less

  20. [Characterization of High Andean forest edges and implications for their ecological restoration (Colombia)].

    Science.gov (United States)

    Montenegro, Alba Lucía; Vargas Ríos, Orlando

    2008-09-01

    The growth of a forest patch through colonization of the adjacent matrix is mostly determined by the particular characteristics of the edge zone. Knowing how these characteristics are related to a specific edge type and how they influence the regeneration process, is important for High Andean forest edges restoration. This study aimed to characterize three types of High Andean forest edge in Cogua Forest Reserve (Colombia): 1) edge of Chusquea scandens, 2) "paramizado", and 3) old edge, characterized for being in a later successional state. Two forest patches were chosen for each edge type and 13 criteria were analyzed; these were of topographic order, micro-environmental order, vegetation structure and species composition. In each patch the vegetation was evaluated by means of two 60 m transects perpendicular to the edge and along the matrix-edge-interior of the forest gradient. All woody plant species were identified and counted to determine their abundance. Environmental variables (air temperature, relative humidity, wind speed, and light radiation) were measured in one of the transects. Three of the 13 criteria were of little importance in shaping the type of edge habitat (slope, patch shape and area). The others were closely related with the micro-environmental conditions and in turn with the vegetation structure and composition; this relationship confers particular characteristics to each edge type. The microclimate and floristic edge limits coincided; edges extend between 10 and 20 m into the forest depending on the edge type. The paramizado edge has the smallest environmental self-regulation capacity and is more exposed to fluctuations of the studied variables, because of its greatest exposition to the wind action and loss of the tallest trees (between 10 and 15 m) which regulate the understorey microclimate. This low environmental buffer capacity prevents the establishing of mature forest species (for example, Schefflera sp. and Oreopanax bogotensis

  1. Climatic and lacustrine morphometric controls of diatom paleoproductivity in a tropical Andean lake

    Science.gov (United States)

    Bao, R.; Hernández, A.; Sáez, A.; Giralt, S.; Prego, R.; Pueyo, J. J.; Moreno, A.; Valero-Garcés, B. L.

    2015-12-01

    The coupling of lake dynamics with catchment biogeochemistry is considered the key element controlling primary production in mountain lakes at time scales of a few decades to millennia, yet little is known on the impacts of the morphometry of lakes throughout their ontogeny. As Lake Chungará (Central Andean Altiplano, northern Chile) experienced long-term lake-level fluctuations that strongly modified its area:volume ratio, it is an ideal system for exploring the relative roles that long-term climatic shifts and lake morphometry play on biosiliceous lacustrine productivity. In this paper, we review previous data on the percent contents of total organic carbon, total inorganic carbon, total nitrogen, total biogenic silica, isotopic composition of organic matter, carbonates, and diatom frustules, as well as data on the abundance of the chlorophycean Botryococcus braunii in this lake for the period 12,400-1300 cal yr BP. We also include new data on organic carbon and biogenic silica mass accumulation rates and the diatom assemblage composition of an offshore core dated using 14C and U/Th. Biosiliceous productivity in Lake Chungará was influenced by shifts in allochthonous nutrient inputs related to variability in precipitation. Humid phases dated at approx. 12,400 to 10,000 and 9600 to 7400 cal yr BP coincide with periods of elevated productivity, whereas decreases in productivity were recorded during arid phases dated at approx. 10,000 to 9600 and 7400 to 3550 cal yr BP (Andean mid-Holocene Aridity Period). However, morphometry-related in-lake controls led to a lack of a linear response of productivity to precipitation variability. During the late Glacial to early Holocene, lowstands facilitated complete water column mixing, prompting episodic massive blooms of a large centric diatom, Cyclostephanos cf. andinus. Thus, moderate productivity could be maintained, regardless of aridity, by this phenomenon of morphometric eutrophy during the early history of the lake

  2. Genetics and mapping of a new anthracnose resistance locus in Andean common bean Paloma.

    Science.gov (United States)

    de Lima Castro, Sandra Aparecida; Gonçalves-Vidigal, Maria Celeste; Gilio, Thiago Alexandre Santana; Lacanallo, Giselly Figueiredo; Valentini, Giseli; da Silva Ramos Martins, Vanusa; Song, Qijian; Galván, Marta Zulema; Hurtado-Gonzales, Oscar P; Pastor-Corrales, Marcial Antonio

    2017-04-18

    The Andean cultivar Paloma is resistant to Mesoamerican and Andean races of Colletotrichum lindemuthianum, the fungal pathogen that causes the destructive anthracnose disease in common bean. Remarkably, Paloma is resistant to Mesoamerican races 2047 and 3481, which are among the most virulent races of the anthracnose pathogen. Most genes conferring anthracnose resistance in common bean are overcome by these races. The genetic mapping and the relationship between the resistant Co-Pa gene of Paloma and previously characterized anthracnose resistance genes can be a great contribution for breeding programs. The inheritance of resistance studies for Paloma was performed in F 2 population from the cross Paloma (resistant) × Cornell 49-242 (susceptible) inoculated with race 2047, and in F 2 and F 2:3 generations from the cross Paloma (resistant) × PI 207262 (susceptible) inoculated with race 3481. The results of these studies demonstrated that a single dominant gene confers the resistance in Paloma. Allelism tests performed with multiple races of C. lindemuthianum showed that the resistance gene in Paloma, provisionally named Co-Pa, is independent from the anthracnose resistance genes Co-1, Co-2, Co-3, Co-4, Co-5, Co-6, Co-12, Co-13, Co-14, Co-15 and Co-16. Bulk segregant analysis using the SNP chip BARCBean6K_3 positioned the approximate location of Co-Pa in the lower arm of chromosome Pv01. Further mapping analysis located the Co-Pa gene at a 390 kb region of Pv01 flanked by SNP markers SS82 and SS83 at a distance of 1.3 and 2.1 cM, respectively. The results presented here showed that Paloma cultivar has a new dominant gene conferring resistance to anthracnose, which is independent from those genes previously described. The linkage between the Co-Pa gene and the SS82 and SS83 SNP markers will be extremely important for marker-assisted introgression of the gene into elite cultivars in order to enhance resistance.

  3. Complex land cover change, water and sediment yield in a degraded Andean environment

    Science.gov (United States)

    Molina, Armando; Vanacker, Veerle; Balthazar, Vincent; Mora, Diego; Govers, Gerard

    2012-11-01

    SummaryRapid land use/-cover change has increasingly transformed the hydrological functioning of tropical Andean ecosystems. The hydrological response to forest cover change strongly depends on the initial state of the ecosystem. Relatively little is known about human-disturbed ecosystems where forest plantations have been established on highly degraded land. In this paper, we analyze the impact of forest change on water and sediment fluxes for a highly degraded Andean catchment. Different pathways of land cover change (1963-2007) are observed in the Jadan catchment, with deforestation taking place in remote uplands and recovery and reforestation in the middle and lower parts where agricultural and bare lands are prevalent. Time series analyses of streamflow and rainfall data (1979/1982-2005/2007) show significant shifts in the distribution of rainfall and flow data. Changes in discharge are not resulting from changes in precipitation, as the direction of change is opposite. The removal of native forest for rangeland or croplands (by -20 km2) is likely to have contributed to the increase in total annual water yield, through an increase in annual baseflow by 25 mm. The observed changes in peakflow are important as the 1st percentile highest flow rates were 54% lower, while the 1st percentile rainfall amounts increased by 52%. The observed decrease in peakflow cannot be explained by clearcut of native forest, but is likely to be related to reforestation of degraded lands as well as spontaneous recovery of vegetation on remaining grazing lands. Over the same time period, a major decrease in specific sediment yields and suspended sediment loads was observed. Although deforestation in the upper parts led to increased landslide activity, this change is not reflected in an increased sediment yield. Small upland rivers are often nearly completely blocked by landslide material, thereby reducing their potential to transport sediment. In contrast, the reduction in estimated

  4. How can we improve Mediterranean cropping systems?

    DEFF Research Database (Denmark)

    Benlhabib, O.; Yazar, A.; Qadir, M.

    2014-01-01

    In the Mediterranean region, crop productivity and food security are closely linked to the adaptation of cropping systems to multiple abiotic stresses. Limited and unpredictable rainfall and low soil fertility have reduced agricultural productivity and environmental sustainability. For this reason...... the tested interventions, incorporation of crop residues coupled with supplementary irrigation showed a significantly positive effect on crop productivity, yield stability and environmental sustainability....

  5. Alternatives to crop residues for soil amendment

    OpenAIRE

    Powell, J.M.; Unger, P.W.

    1997-01-01

    Metadata only record In semiarid agroecosystems, crop residues can provide important benefits of soil and water conservation, nutrient cycling, and improved subsequent crop yields. However, there are frequently multiple competing uses for residues, including animal forage, fuel, and construction material. This chapter discusses the various uses of crop residues and examines alternative soil amendments when crop residues cannot be left on the soil.

  6. Crop rotation modelling - A European model intercomparison

    DEFF Research Database (Denmark)

    Kollas, Chris; Kersebaum, Kurt C; Nendel, Claas

    2015-01-01

    Diversification of crop rotations is considered an option to increase the resilience of European crop production under climate change. So far, however, many crop simulation studies have focused on predicting single crops in separate one-year simulations. Here, we compared the capability of fiftee...

  7. 77 FR 22467 - Common Crop Insurance Regulations; Fresh Market Tomato (Dollar Plan) Crop Provisions

    Science.gov (United States)

    2012-04-16

    ...-0006] RIN 0563-AC32 Common Crop Insurance Regulations; Fresh Market Tomato (Dollar Plan) Crop... Insurance Corporation (FCIC) finalizes the Common Crop Insurance Regulations, Fresh Market Tomato (Dollar... Common Crop Insurance Regulations (7 CFR part 457), Fresh Market Tomato (Dollar Plan) Crop Provisions...

  8. 76 FR 71276 - Common Crop Insurance Regulations; Pecan Revenue Crop Insurance Provisions

    Science.gov (United States)

    2011-11-17

    ...-0008] RIN 0563-AC35 Common Crop Insurance Regulations; Pecan Revenue Crop Insurance Provisions AGENCY... Corporation (FCIC) proposes to amend the Common Crop Insurance Regulations, Pecan Revenue Crop Insurance... Regulations (7 CFR part 457) by revising Sec. 457.167 Pecan Revenue Crop Insurance Provisions, to be effective...

  9. 75 FR 15603 - Common Crop Insurance Regulations; Florida Avocado Crop Insurance Provisions

    Science.gov (United States)

    2010-03-30

    ... to: (1) Theft; or (2) Inability to market the avocados for any reason other than actual physical... Crop Insurance Regulations; Florida Avocado Crop Insurance Provisions AGENCY: Federal Crop Insurance... Common Crop Insurance Regulations; Florida Avocado Crop Insurance Provisions to convert the Florida...

  10. Beans (Phaseolus ssp.) as a Model for Understanding Crop Evolution

    Science.gov (United States)

    Bitocchi, Elena; Rau, Domenico; Bellucci, Elisa; Rodriguez, Monica; Murgia, Maria L.; Gioia, Tania; Santo, Debora; Nanni, Laura; Attene, Giovanna; Papa, Roberto

    2017-01-01

    Here, we aim to provide a comprehensive and up-to-date overview of the most significant outcomes in the literature regarding the origin of Phaseolus genus, the geographical distribution of the wild species, the domestication process, and the wide spread out of the centers of origin. Phaseolus can be considered as a unique model for the study of crop evolution, and in particular, for an understanding of the convergent phenotypic evolution that occurred under domestication. The almost unique situation that characterizes the Phaseolus genus is that five of its ∼70 species have been domesticated (i.e., Phaseolus vulgaris, P. coccineus, P. dumosus, P. acutifolius, and P. lunatus), and in addition, for P. vulgaris and P. lunatus, the wild forms are distributed in both Mesoamerica and South America, where at least two independent and isolated episodes of domestication occurred. Thus, at least seven independent domestication events occurred, which provides the possibility to unravel the genetic basis of the domestication process not only among species of the same genus, but also between gene pools within the same species. Along with this, other interesting features makes Phaseolus crops very useful in the study of evolution, including: (i) their recent divergence, and the high level of collinearity and synteny among their genomes; (ii) their different breeding systems and life history traits, from annual and autogamous, to perennial and allogamous; and (iii) their adaptation to different environments, not only in their centers of origin, but also out of the Americas, following their introduction and wide spread through different countries. In particular for P. vulgaris this resulted in the breaking of the spatial isolation of the Mesoamerican and Andean gene pools, which allowed spontaneous hybridization, thus increasing of the possibility of novel genotypes and phenotypes. This knowledge that is associated to the genetic resources that have been conserved ex situ and in

  11. Starch Biosynthesis in Crop Plants

    Directory of Open Access Journals (Sweden)

    Ian J. Tetlow

    2018-05-01

    Full Text Available Starch is a water-insoluble polyglucan synthesized inside the plastids of plant tissues to provide a store of carbohydrate. Starch harvested from plant storage organs has probably represented the major source of calories for the human diet since before the dawn of civilization. Following the advent of agriculture and the building of complex societies, humans have maintained their dependence on high-yielding domesticated starch-forming crops such as cereals to meet food demands, livestock production, and many non-food applications. The top three crops in terms of acreage are cereals, grown primarily for the harvestable storage starch in the endosperm, although many starchy tuberous crops also provide an important source of calories for various communities around the world. Despite conservation in the core structure of the starch granule, starches from different botanical sources show a high degree of variability, which is exploited in many food and non-food applications. Understanding the factors underpinning starch production and its final structure are of critical importance in guiding future crop improvement endeavours. This special issue contains reviews on these topics and is intended to be a useful resource for researchers involved in improvement of starch-storing crops.

  12. Macrophyte Communities of Andean Rivers: Composition and Relation with Environmental Factors

    Directory of Open Access Journals (Sweden)

    Alida Marcela Gómez Rodríguez

    2017-01-01

    Full Text Available Small streams of tropical Andes have been poorly studied. Therefore, there is little information about the structure, dynamics and function of their macrophyte communities. In this research, aquatic plant communities of 18 Andean streams of La Vieja (Quindío and Otún (Risaralda river basins were studied; those are some of the basins most affected by anthropic activities in the country. Streams were selected according to their association with the main land’s uses of the region in both basins. The aim of the study was to evaluate the effect of land use on the structure of macrophyte communities. Streams running exclusively through each land use were selected. Sampling was done in two different climatic seasons of year 2006. Vegetation found (54 species belonging to 25 families was dominated by species with high capability of adaptation to changing and disturbed environments. Richness and abundance of macrophytes were lower than those reported in other tropical aquatic systems. Variables associated with land use, such as temperature, conductivity and type of substrate of the streams mainly explained the structure of the macrophyte communities: streams running on meat-cattle areas -with higher temperatures, conductivity and dominance of sandy-slimy substrates- had higher macrophyte species richness and abundance than streams of protected-forest areas, with higher coverage by riparian vegetation, lower temperatures and conductivity and rocky substrates.

  13. Diversification of the rainfrog Pristimantis ornatissimus in the lowlands and Andean foothills of Ecuador.

    Directory of Open Access Journals (Sweden)

    Juan M Guayasamin

    Full Text Available Geographic barriers and elevational gradients have long been recognized as important in species diversification. Here, we illustrate an example where both mechanisms have shaped the genetic structure of the Neotropical rainfrog, Pristimantis ornatissimus, which has also resulted in speciation. This species was thought to be a single evolutionary lineage distributed throughout the Ecuadorian Chocó and the adjacent foothills of the Andes. Based on recent sampling of P. ornatissimus sensu lato, we provide molecular and morphological evidence that support the validity of a new species, which we name Pristimantis ecuadorensis sp. nov. The sister species are elevational replacements of each other; the distribution of Pristimantis ornatissimus sensu stricto is limited to the Ecuadorian Chocó ecoregion (< 1100 m, whereas the new species has only been found at Andean localities between 1450-1480 m. Given the results of the Multiple Matrix Regression with Randomization analysis, the genetic difference between P. ecuadorensis and P. ornatissimus is not explained by geographic distance nor environment, although environmental variables at a finer scale need to be tested. Therefore this speciation event might be the byproduct of stochastic historic extinction of connected populations or biogeographic events caused by barriers to dispersal such as rivers. Within P. ornatissimus sensu stricto, morphological patterns and genetic structure seem to be related to geographic isolation (e.g., rivers. Finally, we provide an updated phylogeny for the genus, including the new species, as well as other Ecuadorian Pristimantis.

  14. Diversification of the rainfrog Pristimantis ornatissimus in the lowlands and Andean foothills of Ecuador

    Science.gov (United States)

    Hutter, Carl R.; Tapia, Elicio E.; Culebras, Jaime; Peñafiel, Nicolás; Pyron, R. Alexander; Morochz, Carlos; Funk, W. Chris; Arteaga, Alejandro

    2017-01-01

    Geographic barriers and elevational gradients have long been recognized as important in species diversification. Here, we illustrate an example where both mechanisms have shaped the genetic structure of the Neotropical rainfrog, Pristimantis ornatissimus, which has also resulted in speciation. This species was thought to be a single evolutionary lineage distributed throughout the Ecuadorian Chocó and the adjacent foothills of the Andes. Based on recent sampling of P. ornatissimus sensu lato, we provide molecular and morphological evidence that support the validity of a new species, which we name Pristimantis ecuadorensis sp. nov. The sister species are elevational replacements of each other; the distribution of Pristimantis ornatissimus sensu stricto is limited to the Ecuadorian Chocó ecoregion (< 1100 m), whereas the new species has only been found at Andean localities between 1450–1480 m. Given the results of the Multiple Matrix Regression with Randomization analysis, the genetic difference between P. ecuadorensis and P. ornatissimus is not explained by geographic distance nor environment, although environmental variables at a finer scale need to be tested. Therefore this speciation event might be the byproduct of stochastic historic extinction of connected populations or biogeographic events caused by barriers to dispersal such as rivers. Within P. ornatissimus sensu stricto, morphological patterns and genetic structure seem to be related to geographic isolation (e.g., rivers). Finally, we provide an updated phylogeny for the genus, including the new species, as well as other Ecuadorian Pristimantis. PMID:28329011

  15. Effects of light intensity and fertilization on the growth of Andean Oak Seedlings at Nursery

    International Nuclear Information System (INIS)

    Sepulveda, Yira Lucia; Diez, Maria Claudia; Moreno, Flavio Humberto; Leon, Juan Diego; Walter Osorio, Nelson

    2014-01-01

    Quercus humboldtii is a native plant species of great importance in Colombia for use in reforestation and restoration of degraded Andean highlands. The species is highly threatened and it is necessary to establish programs of propagation and planting. However, little is known about their nutritional and light requirements. The aim of this study was to determine the effects of single and combined relative illumination (IR) and fertilization on the growth of seedlings of Q. humboldtii at nursery. For this purpose three contrasting ir regimes (high, medium, and low ir) and nine fertilization treatments were established: complete (TC), a missing nutrient (-N,-P,-K,-Ca,-Mg, -S,-B) and a control without fertilization (TO). The best development of seedlings was showed in the medium ir condition. All treatments with a lacking nutrient showed decreases in seedling development regarding TC, except in the -B treatment. Nitrogen was the most limiting nutrient yielding biomass similar to that of TO. The impact of nutrient limitation on seedling performance was in the following order:-N>-Ca,-K,-P>-Mg,-S>-B. no significant interaction IR x fertilization was detected on seedling development.

  16. Investigation Into the Humaneness of Slaughter Methods for Guinea Pigs (Cavia porcelus) in the Andean Region.

    Science.gov (United States)

    Limon, Georgina; Gonzales-Gustavson, Eloy A; Gibson, Troy J

    2016-01-01

    Guinea pigs (Cavia porcelus) are an important source of nonhuman animal protein in the Andean region of South America. Specific guidelines regarding the welfare of guinea pigs before and during slaughter have yet to be developed. This study critically assessed the humaneness of 4 different stunning/slaughter methods for guinea pigs: cervical neck dislocation (n = 60), electrical head-only stunning (n = 83), carbon dioxide (CO2) stunning (n = 21), and penetrating captive bolt (n = 10). Following cervical neck dislocation, 97% of guinea pigs had at least 1 behavioral or cranial/spinal response. Six percent of guinea pigs were classified as mis-stunned after electrical stunning, and 1% were classified as mis-stunned after captive bolt. Increased respiratory effort was observed during CO2 stunning. Apart from this finding, there were no other obvious behavioral responses that could be associated with suffering. Of the methods assessed, captive bolt was deemed the most humane, effective, and practical method of stunning guinea pigs. Cervical neck dislocation should not be recommended as a slaughter method for guinea pigs.

  17. Repeated evolution of vertebrate pollination syndromes in a recently diverged Andean plant clade.

    Science.gov (United States)

    Lagomarsino, Laura P; Forrestel, Elisabeth J; Muchhala, Nathan; Davis, Charles C

    2017-08-01

    Although specialized interactions, including those involving plants and their pollinators, are often invoked to explain high species diversity, they are rarely explored at macroevolutionary scales. We investigate the dynamic evolution of hummingbird and bat pollination syndromes in the centropogonid clade (Lobelioideae: Campanulaceae), an Andean-centered group of ∼550 angiosperm species. We demonstrate that flowers hypothesized to be adapted to different pollinators based on flower color fall into distinct regions of morphospace, and this is validated by morphology of species with known pollinators. This supports the existence of pollination syndromes in the centropogonids, an idea corroborated by ecological studies. We further demonstrate that hummingbird pollination is ancestral, and that bat pollination has evolved ∼13 times independently, with ∼11 reversals. This convergence is associated with correlated evolution of floral traits within selective regimes corresponding to pollination syndrome. Collectively, our results suggest that floral morphological diversity is extremely labile, likely resulting from selection imposed by pollinators. Finally, even though this clade's rapid diversification is partially attributed to their association with vertebrate pollinators, we detect no difference in diversification rates between hummingbird- and bat-pollinated lineages. Our study demonstrates the utility of pollination syndromes as a proxy for ecological relationships in macroevolutionary studies of certain species-rich clades. © 2017 The Author(s). Evolution © 2017 The Society for the Study of Evolution.

  18. Vascular Endothelial Growth Factor-A Is Associated with Chronic Mountain Sickness in the Andean Population

    Science.gov (United States)

    Espinoza, Jose R.; Alvarez, Giancarlo; León-Velarde, Fabiola; Ju Preciado, Hugo F.; Macarlupu, Jose-Luis; Rivera-Ch, Maria; Rodriguez, Jorge; Favier, Judith; Gimenez-Roqueplo, Anne-Paule

    2014-01-01

    Abstract Espinoza, Jose R., Giancarlo Alvarez, Fabiola León-Velarde, Hugo F. Ju Preciado, Jose-Luis Macarlupu, Maria Rivera-Ch, Jorge Rodriguez, Judith Favier, Anne-Paule Gimenez-Roqueplo, and Jean-Paul Richalet. Vascular endothelial growth factor-A is associated with chronic mountain sickness in Andean population. High Alt Med Biol. 15:146–154, 2014.—A study of chronic mountain sickness (CMS) with a candidate gene—vascular endothelial growth factor A (VEGFA)—was carried out in a Peruvian population living at high altitude in Cerro de Pasco (4380 m). The study was performed by genotyping of 11 tag SNPs encompassing 2.2 kb of region of VEGFA gene in patients with a diagnosis of CMS (n=131; 49.1±12.7 years old) and unrelated healthy controls (n=84; 47.2±13.4 years old). The VEGFA tag SNP rs3025033 was found associated with CMS (p0.36, p<0.01), suggesting selection is operating on the VEGF gene. Our results suggest that VEGFA is associated with CMS in long-term residents at high altitude in the Peruvian Andes. PMID:24971768

  19. Amazonian Amphibian Diversity Is Primarily Derived from Late Miocene Andean Lineages

    Science.gov (United States)

    Santos, Juan C; Coloma, Luis A; Summers, Kyle; Caldwell, Janalee P; Ree, Richard; Cannatella, David C

    2009-01-01

    The Neotropics contains half of remaining rainforests and Earth's largest reservoir of amphibian biodiversity. However, determinants of Neotropical biodiversity (i.e., vicariance, dispersals, extinctions, and radiations) earlier than the Quaternary are largely unstudied. Using a novel method of ancestral area reconstruction and relaxed Bayesian clock analyses, we reconstructed the biogeography of the poison frog clade (Dendrobatidae). We rejected an Amazonian center-of-origin in favor of a complex connectivity model expanding over the Neotropics. We inferred 14 dispersals into and 18 out of Amazonia to adjacent regions; the Andes were the major source of dispersals into Amazonia. We found three episodes of lineage dispersal with two interleaved periods of vicariant events between South and Central America. During the late Miocene, Amazonian, and Central American-Chocoan lineages significantly increased their diversity compared to the Andean and Guianan-Venezuelan-Brazilian Shield counterparts. Significant percentage of dendrobatid diversity in Amazonia and Chocó resulted from repeated immigrations, with radiations at Venezuelan Highlands, and Guiana Shield have undergone extended in situ diversification at near constant rate since the Oligocene. The effects of Miocene paleogeographic events on Neotropical diversification dynamics provided the framework under which Quaternary patterns of endemism evolved. PMID:19278298

  20. Large-scale patterns of turnover and Basal area change in Andean forests.

    Directory of Open Access Journals (Sweden)

    Selene Báez

    Full Text Available General patterns of forest dynamics and productivity in the Andes Mountains are poorly characterized. Here we present the first large-scale study of Andean forest dynamics using a set of 63 permanent forest plots assembled over the past two decades. In the North-Central Andes tree turnover (mortality and recruitment and tree growth declined with increasing elevation and decreasing temperature. In addition, basal area increased in Lower Montane Moist Forests but did not change in Higher Montane Humid Forests. However, at higher elevations the lack of net basal area change and excess of mortality over recruitment suggests negative environmental impacts. In North-Western Argentina, forest dynamics appear to be influenced by land use history in addition to environmental variation. Taken together, our results indicate that combinations of abiotic and biotic factors that vary across elevation gradients are important determinants of tree turnover and productivity in the Andes. More extensive and longer-term monitoring and analyses of forest dynamics in permanent plots will be necessary to understand how demographic processes and woody biomass are responding to changing environmental conditions along elevation gradients through this century.

  1. Insects associated with exposed decomposing bodies in the Colombian Andean Coffee Region

    Directory of Open Access Journals (Sweden)

    Diana Grisales

    2010-01-01

    Full Text Available In Colombia, mainly classic forensic medicine methods were used to clarify crimes until 2004. However, other disciplines, including forensic entomology, started to be considered only after the New Accusatory System introduction in Bogotá and the Coffee Region in 2005. In order to provide tools for obtaining evidentiary material elements in judicial trials, it is presented here the succession of insects throughout the decomposition process of an exposed carcass of Sus scrofa Linnaeus 1758 (Suidae and the Occurrence Matrix of colonizing species. This process was evaluated under ambient conditions in the Andean rural area of the city of Pereira, in the Mundo Nuevo district, located in a pre-montane Wet Forest area, from October to November 2006. A sampling period of 27 days and 3198 individuals were collected. We found these colonizing species in the following stages of decomposition: Lucilia eximia (Wiedemann, 1819 fresh; Hemilucilia semidiaphana (Rondani, 1850, Oxelytrum discicolle (Brullé, 1840, and Cochliomyia macellaria (Fabricius 1775 bloated; Chrysomya albiceps (Wiedemann 1819, Compsomyiops verena (Walker, 1849, Ophyra aenescens (Wiedemann, 1830 and Musca domestica Linnaeus, 1758 active; Fannia sp. advanced and Stearibia nigriceps (Meigen, 1826 remains. This study provides support tools to define the Post Mortem Interval that may be used by experts from government institutions and laboratories officially accredited.

  2. Vicuña conservation and poverty alleviation? Andean communities and international fibre markets

    Directory of Open Access Journals (Sweden)

    Gabriela Lichtenstein

    2009-09-01

    Full Text Available Vicuna (Vicugna vicugna fiber is produced by extremely low-income communities that inhabit the harsh environment of the Andes in Argentina, Chile, Peru and Bolivia. At the other end of the social scale, affluent consumers are willing to pay high prices for vicuna-made accessories and clothes. Vicuna management projects follow the logic of community-based wildlife management. The rationale for seeking to conserve vicuñas through sustainable use is that commercial utilization of the fiber (obtained from live-shorn animals will generate sufficient economic benefits to outweigh the costs of conservation, and contribute to community development and poverty alleviation. However, although conservation efforts have been extremely successful with vicuñas having recovered from the brink of extinction, the socio-economic achievements have thus far proved modest. This paper explores multiple-objective projects that address vicuña conservation and poverty alleviation in Andean countries. In doing so it analyses the tensions that exist between these objectives, as well as the factors that limit a more equitable distribution of benefits among stakeholders. Examples are drawn from vicuna management under common-property in Peru and Bolivia, and vicuna captive management under private property in Argentina. These case studies enable us to illustrate the complex relationship between local communities and the global market, and the importance of community enterprises and supportive government policy in managing a common pool resource.

  3. A phytoremediation approach using Calamagrostis ligulata and Juncus imbricatus in Andean wetlands of Peru.

    Science.gov (United States)

    Miguel, Bobadilla; Edell, Aliaga; Edson, Yupanqui; Edwin, Palomino

    2013-01-01

    Emergent plant species growing in Andean natural wetlands have shown efficient phytoremediation capabilities in wetlands polluted by acid mine drainage. However, the types and amounts of heavy metals accumulated by native plant species are not well understood. In this study, we focused on determining heavy metal concentrations and bioaccumulation factors in Calamagrostis ligulata and Juncus imbricatus. Two acid wetlands located above 3,500 m a.s.l. in Ancash, Peru were assessed. Physico-chemical parameters and heavy metals concentrations in control and experimental plant samples were measured in dry and rainy seasons. Results indicated that C. ligulata and J. imbricatus aerial parts accumulated higher amounts of Fe, Zn, As and Al. Also, bioaccumulation factors revealed notable increases in As, Pb and Al, but less so in Cd, Fe and Zn. On the other hand, physico-chemical parameters of water quality (pH, temperature, dissolved oxygen, sulphides) between inflow and outflow of wetlands indicated significant differences in the presence of metals in comparison with their maximum permissible limits. Both emergent plant species showed an accumulation of heavy metals and thus the ability to recovery of water quality in wetland outflows.

  4. [Floristic composition and distribution of the Andean subtropical riparian forests of Lules River, Tucuman, Argentina].

    Science.gov (United States)

    Sirombra, Martín G; Mesa, Leticia M

    2010-03-01

    We studied the floristic composition and distribution of the riparian forest of two hydrographical systems in a subtropical Andean region. Using uni and multivariate techniques, we tested the hypotheses that a differentiable riparian forest exists, composed by native vegetation typical of the Yungas phytogeographical province, and that the distribution of vegetation varied significantly with geomorphologic characteristics. Parallel transects along the water courses were used to collect presence-absence data of vegetation in eleven sites. Detrended Correspondence Analysis defined a group of common riparian species for the studied area (Solanum riparium, Phenax laevigatus, Tipuana tipu, Cestrum parqui, Carica quercifolia, Acacia macracantha, Celtis iguanaea, Juglans australis, Pisoniella arborescens, Baccharis salicifolia, Cinnamomum porphyrium and Eugenia uniflora) and identified two reference sites. The distribution of the riparian vegetation varied significantly with the geomorphic characteristics along the studied sites. Riparian habitats were composed by native and exotic species. A distinct riparian flora, different in structure and function from adjacent terrestrial vegetation, could not be identified. Riparian species were similar to the adjacent terrestrial strata. These species would not be limited by the proximity to the river. Anthropogenic impacts were important factors regulating the introduction and increase of exotic vegetation. The lack of regulation of some activities in the zone could cause serious problems in the integrity of this ecosystem.

  5. Morphology, nectar characteristics and avian pollinators in five Andean Puya species (Bromeliaceae)

    Science.gov (United States)

    Hornung-Leoni, C. T.; González-Gómez, P. L.; Troncoso, A. J.

    2013-08-01

    Five Andean Puya species (Puya alpestris, Puya chilensis, Puya coerulea, Puya raimondii and Puya venusta) were studied to determine the relationship between their avian visitors, and plant morphology and nectar characteristics. Our results showed a significant relationship between nectar concentration, presence of sterile apex and avian pollinators's species. In contrast, nectar composition was not related to the frequency of avian visits. We found that Puya species were mainly visited by specialist nectarivorous birds such as hummingbirds (i.e., P. coerulea and P. venusta), lacked a sterile apex and produced high nectar concentration in low volumes. In contrast, species mainly visited by generalist passerines (i.e., P. chilensis and P. alpestris) were characterized by the presence of a sterile apex and production of highly diluted nectar in large volumes. In a mono-specific group we found that P. raimondii produces highly concentrated nectar in large volumes, and its flowers were visited by hummingbirds and passerine birds. We found no effect of nectar composition on bird's visits. Our study highlights the interplay between morphological traits, nectar characteristics and the ecological framework to explain specialized and generalized birds pollination systems.

  6. Quality characterization of Andean blackberry fruits (Rubus glaucus Benth. in different maturity stages in Antioquia, Colombia

    Directory of Open Access Journals (Sweden)

    Catarina Pedro Carvalho

    2015-04-01

    Full Text Available Product quality and safety are two essential characteristics for the fruit market, making it necessary to normalize and standardize processes in order to improve their commercialization. In this study, the quality of two Andean blackberry cultivars grown in two regions of Antioquia (Envigado and Guarne, Colombia, from different maturity stages as defined by the Norma Tecnica Colombiana 7146 (NTC Spanish was characterized. The parameters that were found suitable for the fruit quality characterization were: weight, total solid soluble content (TSS, titratable acidity (TA, maturity index (MI, color index (CI and firmness (F. The equatorial diameter (ED maintained its importance relative to the standard and the market, along with the juice yield (JY. The quotient a*/b* presented the best correlation with the visual color scale as defined in the standard. The TSS ranges defined in NTC 4106 were not verified in this studied for fruits grown in the agro-climatic conditions of Antioquia. Linear regression models are a useful tool for making quick and easy comparisons and estimations of the quality parameters.

  7. Mineral content in soil and pasture in bovine dairy herds of the Andean region of Ecuador

    Directory of Open Access Journals (Sweden)

    Luís Rodrigo Balarezo Urresta

    2017-10-01

    Full Text Available The objective of this research was to characterize the mineral status of the soil and pasture in of the Andean Ecuadorian region, during the rainy and dry periods, three dairy farms were used as study cases investigated him three dairy farms of the El Carchi province. They determined the chemical indicators of the soil and the pasture, the descriptive statisticians were calculated themselves and it was used a multifactorial ANOVA to determine the main factors affecting them on them, comparing means with Bonferroni and Duncan test. The soil classified as acid lightly, 100 % of the samples presented elevated levels of organic matter, NH4+, Mg, Cu, Zn, Fe and Mn. The farm had a significant effect on the pH, Ca, Mg, K, Cu, Fe, Mg and P, and the climatic period on the organic matter, NH4+, S, Cu and P. Pasture presented deficiencies of Mg, Zn and Na, the other minerals were above the critical limits. The farm affected the Ca, P, Mg, Na and Mn, and the climatic period the levels of Ca, K, Cu y Zn. In conclusion, 100 % soil samples presented high OM, slight acidity, low levels of Ca and high concentrations of NH4+, S, Mg, Cu, Zn and Mn. In pastures, there were diagnosed deficiencies of P, Cu and Zn, and their concentrations differed among farms and the two climatic periods of the year.

  8. Revisiting the Andean butterfly Eryphanis zolvizora group (Lepidoptera, Nymphalidae: one or several species?

    Directory of Open Access Journals (Sweden)

    Patrick Blandin

    2014-01-01

    Full Text Available Eryphanis zolvizora (Hewitson, 1877 is a rare Andean endemic butterfly, described from Bolivia, which has been historically classified either as a unique species, or as part of a group of three allopatric species from Bolivia, Ecuador and Colombia. In this paper, the group is revised using more than 200 specimens housed in 34 European, and North and South American public and private collections. For the first time, the presence of the group in Western Ecuador and Venezuela is confirmed, and important data on Peruvian populations are provided. In some populations, individual variations of genitalia are observed. Nevertheless, male genitalia allow the distinction of four geographical groups. Considering also habitus characters, eight taxa are distinguished and considered to be subspecies, of which five are new: Eryphanis zolvizora inca ssp. nov., Eryphanis zolvizora chachapoya ssp. nov., Eryphanis zolvizora casagrande ssp. nov.., Eryphanis zolvizora reyi ssp. nov., and Eryphanis zolvizora isabelae ssp. nov.  In the present state of knowledge, these taxa are allopatric, except for a possible geographic overlap in central Peru, where data are insufficient to prove sympatry. The “several subspecies vs. several species” dilemma is discussed, considering its impact for conservation action and policies.

  9. Response Analysis of eight native species of high Andean forest with two methods of propagation

    International Nuclear Information System (INIS)

    Castaneda Sandra Liliana; Garzon Alvaro Ernesto; Cantillo Miguel Angel; Torres Monica Patricia; Silva Luis Jairo

    2007-01-01

    The objective of this research was to generate information on the native tree species represent an alternative in the ecological restoration of the Colombian high Andean forests, according to its dynamo-genetic characteristics. We have chosen and spread the species: Baccharis latifolia (R and P), Bocconia frutescens L., Cordia cylindrostachya (R and P), Diplostephium rosmarinifolium (Benth), Drymis granadensis L f., Eupatorium angustifolium (Kunth), Palicourea vaginata Benth, and Palicourea linearifolia Wernham. The species include a morphological description of flowers, fruits and seeds, and ISTA tests. The spread experiments were made in the nurseries of the Universidad Distrital and La Florida park. For the sexual spread, we have used as treatments four gibberellins concentrations and three shadow conditions, while the vegetative spread consisted of two diameters and ive indol butiric acid (IBA) concentrations. Results have shown that pre-germination treatments are needed for Bocconia frutescens y Palicourea vaginata, in order to increase the probability and germination rate. On the other hand, shadow conditions are needed for Baccharis latifolia, Diplostephium rosmarinifolium, Drymis granadensis, Eupatorium angustifolium and Palicourea vaginata, as their seeds exhibit photoblastic characteristics. Due to the Cordia cylindrostachya and Palicourea linearifolia seed attack by insects (Lepidoptera and Hymenoptera) their sexual spread is highly limited. Finally, regarding the species response to the IBA and diameter combinations, each species responded in a different manner. Additionally, regarding the vegetative spread, the species Bocconia frutescens, Cordia cylindrostachya, Palicourea vaginata, Diplostephium rosmarinifolium and Drymis granadensis were very difficult to spread

  10. The Corregidores of the Colca Valley, Peru: Imperial Administration in an Andean Region

    Directory of Open Access Journals (Sweden)

    Cook, Noble David

    2003-12-01

    Full Text Available The corregidor de los indios was introduced into the Viceroyalty of Peru by Governor García de Castro in 1565. The institution was designed to limit the power of the encomendero elite and to improve administration and justice in the Andean countryside. Here we examine the impact of the reforms at the local level, the corregimiento of Los Collaguas in the Colca Valley, located between Cuzco and Arequipa. Althought the Crown was largely successful in weakening the encomienda, possibility of graft corrupted all but a handful of corregidores. The residencia did check some of those abuses.

    El gobernador García de Castro fue quien introdujo (1565 el corregidor de los indios en el virreinato del Perú. El corregimiento fue establecido para limitar el poder de los encomenderos y mejorar la administración y la justicia en los sitios rurales, y al mismo tiempo incrementar la colección del tributo. En este trabajo examinamos el impacto de las reformas en el corregimiento de los Collaguas situado en el hermoso valle del río Colca entre Arequipa y Cuzco. Aunque la corona fue más exitosa en debilitar la encomienda, la posibilidad de soborno corrompió a la mayoría de los corregidores.

  11. Plant functional groups of potential restoration use in advancing edges of high Andean forests

    International Nuclear Information System (INIS)

    Castellanos Castro, Carolina; Bonilla, Maria Argenis

    2011-01-01

    The study of plant functional groups constitutes a useful tool in the identification of ecological characteristics relevant in community regeneration. The aim of this study was to identify plant's functional groups in high Andean forest advance edges and to evaluate their role during secondary succession in abandoned pasture lands. Based on 10 x 10 m vegetation relevees for the shrubby-arboreal stratum and 1 x 1 m plots for the herbaceous stratum and the revision of vital attributes for each of the species found, this study uses a multivariate approach to construct a trait-based emergent group's classification. The most important attributes in the definition of the groups were the dispersion mechanism and the presence of basal trunk ramification in woody species; in addition differences in the presence of vegetative propagation, specific leaf area index and the ratio height/diameter at breast height were found between groups of the shrubby-arboreal stratum. Four distinct groups were defined in the herbaceous layer and five in the shrubby-arboreal layer, each group contains species with similar colonization strategies. Among the defined groups, the herbaceous species dispersed by various abiotic factors, the shrubby species with basal ramification and dispersed by wind and the species dispersed by birds constitute key strategies in forest recovery in adjacent abandoned pasture lands dominated by Holcus lanatus, and facilitate the establishment of secondary forest species.

  12. Two new Liolaemus lizards from the Andean highlands of Southern Chile (Squamata, Iguania, Liolaemidae)

    Science.gov (United States)

    Troncoso-Palacios, Jaime; Diaz, Hugo A.; Puas, German I.; Riveros-Riffo, Edvin; Elorza, Alvaro A.

    2016-01-01

    Abstract Liolaemus is a diverse genus of lizards, subdivided into two subgenera: Liolaemus (sensu stricto) and Eulaemus, distributed mainly in Chile and Argentina. The Liolaemus elongatus-kriegi complex is the most diverse group within Liolaemus (sensu stricto), especially the species closely related to Liolaemus elongatus, which form a clade currently comprising nine species. Several Chilean species of this group have been recently described, mainly from volcanoes and poorly explored mountains. Here molecular and morphological evidence are provided for a new species of the Liolaemus elongatus clade, which is characterized by its small size and lack of dorsal pattern, unusual features for the species of this group of lizards. Additionally, the lack of precloacal pores in males of Liolaemus (sensu stricto) is a trait found in few species, which do not constitute a monophyletic group. A second new southern Chilean species is also described, without precloacal pores and supported by molecular phylogenetics to be related to Liolaemus villaricensis. Both new species were found in the same locality, near a lake located in a pre-Andean zone with Araucaria and Nothofagus forest. The two species are dedicated to prominent Lonkos (tribal chiefs) of the Mapuche and Pehuenche people: Janequeo and Leftraru. Additionally, the phylogenetic results suggest that Liolaemus lonquimayensis is a synonym of Liolaemus elongatus. PMID:27920609

  13. Faba bean in cropping systems

    DEFF Research Database (Denmark)

    Steen Jensen, Erik; Peoples, Mark B.; Hauggaard-Nielsen, Henrik

    2010-01-01

    The grain legume (pulse) faba bean (Vicia faba L.) is grown world-wide as a protein source for food and feed. At the same time faba bean offers ecosystem services such as renewable inputs of nitrogen (N) into crops and soil via biological N2 fixation, and a diversification of cropping systems. Even...... though the global average grain yield has almost doubled during the past 50 years the total area sown to faba beans has declined by 56% over the same period. The season-to-season fluctuations in grain yield of faba bean and the progressive replacement of traditional farming systems, which utilized...... legumes to provide N to maintain soil N fertility, with industrialized, largely cereal-based systems that are heavily reliant upon fossil fuels (=N fertilizers, heavy mechanization) are some of the explanations for this decline in importance. Past studies of faba bean in cropping systems have tended...

  14. Automated phenotyping of permanent crops

    Science.gov (United States)

    McPeek, K. Thomas; Steddom, Karl; Zamudio, Joseph; Pant, Paras; Mullenbach, Tyler

    2017-05-01

    AGERpoint is defining a new technology space for the growers' industry by introducing novel applications for sensor technology and data analysis to growers of permanent crops. Serving data to a state-of-the-art analytics engine from a cutting edge sensor platform, a new paradigm in precision agriculture is being developed that allows growers to understand the unique needs of each tree, bush or vine in their operation. Autonomous aerial and terrestrial vehicles equipped with multiple varieties of remote sensing technologies give AGERpoint the ability to measure key morphological and spectral features of permanent crops. This work demonstrates how such phenotypic measurements combined with machine learning algorithms can be used to determine the variety of crops (e.g., almond and pecan trees). This phenotypic and varietal information represents the first step in enabling growers with the ability to tailor their management practices to individual plants and maximize their economic productivity.

  15. Helping to increase tree crops

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1970-07-01

    Tree crops such as coffee, coconuts, palm oil, citrus fruits and cocoa are of major importance to the economies of countries in Africa, Asia and Latin America, and may be a prime source of foreign exchange earnings. The search for ways to improve efficiently the yields of crops like these - now being aided by the Division of Atomic Energy in Food and Agriculture operated jointly with the Food and Agriculture Organization - thus has a clearly defined practical goal. D. Nethsinghe deals here with some of the work. (author)

  16. Helping to increase tree crops

    International Nuclear Information System (INIS)

    1970-01-01

    Tree crops such as coffee, coconuts, palm oil, citrus fruits and cocoa are of major importance to the economies of countries in Africa, Asia and Latin America, and may be a prime source of foreign exchange earnings. The search for ways to improve efficiently the yields of crops like these - now being aided by the Division of Atomic Energy in Food and Agriculture operated jointly with the Food and Agriculture Organization - thus has a clearly defined practical goal. D. Nethsinghe deals here with some of the work. (author)

  17. Ammonia volatilization from crop residues and frozen green manure crops

    NARCIS (Netherlands)

    Ruijter, de F.J.; Huijsmans, J.F.M.; Rutgers, B.

    2010-01-01

    Agricultural systems can lose substantial amounts of nitrogen (N). To protect the environment, the European Union (EU) has adopted several directives that set goals to limit N losses. National Emission Ceilings (NEC) are prescribed in the NEC directive for nitrogen oxides and ammonia. Crop residues

  18. Compositions comprising lignosulfonates for crop protection and crop improvement

    NARCIS (Netherlands)

    Stevens, L.H.; Kok, C.J.; Krieken, van der W.M.

    2009-01-01

    International patent application number: WO2004067699http://www.wipo.int/patentscope/search/en/WO2004067699 (EN)The invention relates to a composition for protecting an agricultural crop against external threats, such as weeds, pathogens, abiotic and biotic stresses and/or for improving the quality

  19. Progress update: crop development of biofortified staple food crops ...

    African Journals Online (AJOL)

    Over the past 15 years, biofortification, the process of breeding nutrients into food crops, has gained ample recognition as a cost-effective, complementary, feasible means of delivering micronutrients to populations that may have limited access to diverse diets, supplements, or commercially fortified foods. In 2008, a panel of ...

  20. Validation of crop weather models for crop assessment arid yield ...

    African Journals Online (AJOL)

    IRSIS and CRPSM models were used in this study to see how closely they could predict grain yields for selected stations in Tanzania. Input for the models comprised of weather, crop and soil data collected from five selected stations. Simulation results show that IRSIS model tends to over predict grain yields of maize, ...