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Sample records for anchorage independent functions

  1. Ectodomains of the LDL receptor-related proteins LRP1b and LRP4 have anchorage independent functions in vivo.

    Directory of Open Access Journals (Sweden)

    Martin F Dietrich

    Full Text Available BACKGROUND: The low-density lipoprotein (LDL receptor gene family is a highly conserved group of membrane receptors with diverse functions in developmental processes, lipoprotein trafficking, and cell signaling. The low-density lipoprotein (LDL receptor-related protein 1b (LRP1B was reported to be deleted in several types of human malignancies, including non-small cell lung cancer. Our group has previously reported that a distal extracellular truncation of murine Lrp1b that is predicted to secrete the entire intact extracellular domain (ECD is fully viable with no apparent phenotype. METHODS AND PRINCIPAL FINDINGS: Here, we have used a gene targeting approach to create two mouse lines carrying internally rearranged exons of Lrp1b that are predicted to truncate the protein closer to the N-terminus and to prevent normal trafficking through the secretary pathway. Both mutations result in early embryonic lethality, but, as expected from the restricted expression pattern of LRP1b in vivo, loss of Lrp1b does not cause cellular lethality as homozygous Lrp1b-deficient blastocysts can be propagated normally in culture. This is similar to findings for another LDL receptor family member, Lrp4. We provide in vitro evidence that Lrp4 undergoes regulated intramembraneous processing through metalloproteases and gamma-secretase cleavage. We further demonstrate negative regulation of the Wnt signaling pathway by the soluble extracellular domain. CONCLUSIONS AND SIGNIFICANCE: Our results underline a crucial role for Lrp1b in development. The expression in mice of truncated alleles of Lrp1b and Lrp4 with deletions of the transmembrane and intracellular domains leads to release of the extracellular domain into the extracellular space, which is sufficient to confer viability. In contrast, null mutations are embryonically (Lrp1b or perinatally (Lrp4 lethal. These findings suggest that the extracellular domains of both proteins may function as a scavenger for

  2. Stable Ectopic Expression of ST6GALNAC5 Induces Autocrine MET Activation and Anchorage-Independence in MDCK Cells

    OpenAIRE

    Chia Chu; Donald P Bottaro; Betenbaugh, Michael J.; Joseph Shiloach

    2016-01-01

    The epithelial-mesenchymal transition (EMT) is a complex cancer progression that can boost the metastatic potential of transformed cells by inducing migration, loss of cell adhesion, and promoting proliferation under anchorage-independent conditions. A DNA microarray analysis was performed comparing parental anchorage-dependent MDCK cells and anchorage-independent MDCK cells that were engineered to express human siat7e (ST6GALNAC5). The comparison identified several genes involved in the EMT ...

  3. Overexpressed EDIL3 predicts poor prognosis and promotes anchorage-independent tumor growth in human pancreatic cancer

    Science.gov (United States)

    Feng, Ming-Xuan; Wang, Ya-Hui; Yang, Xiao-Mei; He, Ping; Tian, Guang-Ang; Zhang, Xiao-Xin; Li, Qing; Cao, Xiao-Yan; Huo, Yan-Miao; Yang, Min-Wei; Fu, Xue-Liang; Li, Jiao; Liu, De-Jun; Dai, Miao; Wen, Shan-Yun; Gu, Jian-Ren; Hong, Jie; Hua, Rong; Zhang, Zhi-Gang; Sun, Yong-Wei

    2016-01-01

    Epidermal Growth Factor-like repeats and Discoidin I-Like Domains 3 (EDIL3), an extracellular matrix (ECM) protein associated with vascular morphogenesis and remodeling, is commonly upregulated in multiple types of human cancers and correlates with tumor progression. However, its expression pattern and underlying cellular functions in pancreatic ductal adenocarcinoma (PDAC) remain largely unexplored. In current study, we observed that expression of EDIL3 was significantly up-regulated in PDAC compared with normal controls in both cell lines and clinical specimens. In addition, elevated EDIL3 expression was positively correlated with patients’ TNM stage and T classification. Kaplan-Meier analysis indicated that high EDIL3 expression was significantly associated with shorter overall survival times in PDAC patients. Multivariate Cox regression analysis confirmed EDIL3 expression, age, lymph node metastasis and histological differentiation as independent prognostic factors in PDAC. Knockdown of EDIL3 showed no significant influence on cell viability, migration, invasion and starvation-induced apoptosis, but compromised anoikis resistance and anchorage independent tumor growth of PDAC cells. Meanwhile, treatment with recombinant EDIL3 protein markedly promoted anoikis resistance and anchorage independent tumor growth. Mechanistically, we demonstrated that altered protein expression of Bcl-2 family might contribute to the oncogenic activities of EDIL3. In conclusion, this study provides evidences that EDIL3 is a potential predictor and plays an important role in anchorage independent tumor growth of PDAC and EDIL3-related pathways might represent a novel therapeutic strategy for treatment of pancreatic cancer. PMID:26735172

  4. Stable Ectopic Expression of ST6GALNAC5 Induces Autocrine MET Activation and Anchorage-Independence in MDCK Cells.

    Directory of Open Access Journals (Sweden)

    Chia Chu

    Full Text Available The epithelial-mesenchymal transition (EMT is a complex cancer progression that can boost the metastatic potential of transformed cells by inducing migration, loss of cell adhesion, and promoting proliferation under anchorage-independent conditions. A DNA microarray analysis was performed comparing parental anchorage-dependent MDCK cells and anchorage-independent MDCK cells that were engineered to express human siat7e (ST6GALNAC5. The comparison identified several genes involved in the EMT process that were differentially expressed between the anchorage-dependent and the anchorage-independent MDCK cell lines. The hepatocyte growth factor gene (hgf was found to be over-expressed in the engineered MDCK-siat7e cells at both transcription and protein expression levels. Phosphorylation analysis of the MET receptor tyrosine kinase confirmed the activation of an autocrine loop of the HGF/ MET signaling pathway in the MDCK-siat7e cells. When MET activities were suppressed by using the small-molecular inhibitor drug PF-02341066 (Crizotinib, the anchorage-independent MDCK-siat7e cells reverted to the cellular morphology of the parental anchorage-dependent MDCK cells. These observations indicate that the MET receptor plays a central role in the growth properties of the MDCK cells and its phosphorylation status is likely dependent on sialylation. Further investigation of the downstream signaling targets in the MET network showed that the degree of MDCK cell adhesion correlated with secretion levels of a matrix metalloproteinase, MMP1, suggesting a role of metalloproteinases in the EMT process. These results demonstrate that in addition to its application in biotechnology processes, MDCK-siat7e may serve as a model cell for metastasis studies to decipher the sequence of events leading up to the activation of EMT.

  5. Stable Ectopic Expression of ST6GALNAC5 Induces Autocrine MET Activation and Anchorage-Independence in MDCK Cells.

    Science.gov (United States)

    Chu, Chia; Bottaro, Donald P; Betenbaugh, Michael J; Shiloach, Joseph

    2016-01-01

    The epithelial-mesenchymal transition (EMT) is a complex cancer progression that can boost the metastatic potential of transformed cells by inducing migration, loss of cell adhesion, and promoting proliferation under anchorage-independent conditions. A DNA microarray analysis was performed comparing parental anchorage-dependent MDCK cells and anchorage-independent MDCK cells that were engineered to express human siat7e (ST6GALNAC5). The comparison identified several genes involved in the EMT process that were differentially expressed between the anchorage-dependent and the anchorage-independent MDCK cell lines. The hepatocyte growth factor gene (hgf) was found to be over-expressed in the engineered MDCK-siat7e cells at both transcription and protein expression levels. Phosphorylation analysis of the MET receptor tyrosine kinase confirmed the activation of an autocrine loop of the HGF/ MET signaling pathway in the MDCK-siat7e cells. When MET activities were suppressed by using the small-molecular inhibitor drug PF-02341066 (Crizotinib), the anchorage-independent MDCK-siat7e cells reverted to the cellular morphology of the parental anchorage-dependent MDCK cells. These observations indicate that the MET receptor plays a central role in the growth properties of the MDCK cells and its phosphorylation status is likely dependent on sialylation. Further investigation of the downstream signaling targets in the MET network showed that the degree of MDCK cell adhesion correlated with secretion levels of a matrix metalloproteinase, MMP1, suggesting a role of metalloproteinases in the EMT process. These results demonstrate that in addition to its application in biotechnology processes, MDCK-siat7e may serve as a model cell for metastasis studies to decipher the sequence of events leading up to the activation of EMT. PMID:26848584

  6. Spheroid Culture of Head and Neck Cancer Cells Reveals an Important Role of EGFR Signalling in Anchorage Independent Survival.

    Science.gov (United States)

    Braunholz, Diana; Saki, Mohammad; Niehr, Franziska; Öztürk, Merve; Borràs Puértolas, Berta; Konschak, Robert; Budach, Volker; Tinhofer, Ingeborg

    2016-01-01

    In solid tumours millions of cells are shed into the blood circulation each day. Only a subset of these circulating tumour cells (CTCs) survive, many of them presumable because of their potential to form multi-cellular clusters also named spheroids. Tumour cells within these spheroids are protected from anoikis, which allows them to metastasize to distant organs or re-seed at the primary site. We used spheroid cultures of head and neck squamous cell carcinoma (HNSCC) cell lines as a model for such CTC clusters for determining the role of the epidermal growth factor receptor (EGFR) in cluster formation ability and cell survival after detachment from the extra-cellular matrix. The HNSCC cell lines FaDu, SCC-9 and UT-SCC-9 (UT-SCC-9P) as well as its cetuximab (CTX)-resistant sub-clone (UT-SCC-9R) were forced to grow in an anchorage-independent manner by coating culture dishes with the anti-adhesive polymer poly-2-hydroxyethylmethacrylate (poly-HEMA). The extent of apoptosis, clonogenic survival and EGFR signalling under such culture conditions was evaluated. The potential of spheroid formation in suspension culture was found to be positively correlated with the proliferation rate of HNSCC cell lines as well as their basal EGFR expression levels. CTX and gefitinib blocked, whereas the addition of EGFR ligands promoted anchorage-independent cell survival and spheroid formation. Increased spheroid formation and growth were associated with persistent activation of EGFR and its downstream signalling component (MAPK/ERK). Importantly, HNSCC cells derived from spheroid cultures retained their clonogenic potential in the absence of cell-matrix contact. Addition of CTX under these conditions strongly inhibited colony formation in CTX-sensitive cell lines but not their resistant subclones. Altogether, EGFR activation was identified as crucial factor for anchorage-independent survival of HNSCC cells. Targeting EGFR in CTC cluster formation might represent an attractive anti

  7. Aiolos Promotes Anchorage Independence by Silencing p66Shc Transcription in Cancer Cells

    OpenAIRE

    Li, Xichuan; Xu, Zhao; Du, Wei; Zhang, Zhenfa; Wei, Yiliang; Wang, Hao; Zhu, Zhiyan; Qin, Litao; Wang, Lin; Niu, Qing; Zhao, Xiulan; Girard, Luc; Gong, Yimei; Ma, Zhenyi; Sun, Baocun

    2014-01-01

    Anchorage of tissue cells to their physical environment is an obligate requirement for survival which is lost in mature hematopoietic and in transformed epithelial cells. Here we find that a lymphocyte lineage-restricted transcription factor, Aiolos, is frequently expressed in lung cancers and predicts markedly reduced patient survival. Aiolos decreases expression of a large set of adhesion-related genes, disrupting cell-cell and cell-matrix interactions. Aiolos also reconfigures chromatin st...

  8. Dexamethasone and zinc in combination inhibit the anchorage-independent growth of S-91 Cloudman murine melanoma

    International Nuclear Information System (INIS)

    Zinc inhibited the colony formation of Cloudman S-91 murine melanoma cells in a dose dependent manner with an ID50 of 3.4 μg/ml. Total inhibition of the melanoma colony-forming units occurred at a zinc concentration of 4.42 μg/ml. In the presence of dexamethasone the ID50 for zinc inhibition was reduced by 49% and total inhibition of anchorage-independent growth occurred at the achievable in vivo zinc concentration of 3.0 μg/ml. Dexamethasone and zinc in combination effected a greater than additive inhibition of the murine melanoma colony-forming units. Statistical evaluation of these results showed that zinc and dexamethasone interacted synergistically to inhibit the formation of murine melanoma colonies. 29 references, 1 figure, 1 table

  9. A role for collagen XXIII in cancer cell adhesion, anchorage-independence, and metastasis

    OpenAIRE

    Spivey, Kristin A.; Chung, Ivy; Banyard, Jacqueline; Adini, Irit; Feldman, Henry A.; Bruce R Zetter

    2011-01-01

    Collagen XXIII is a transmembrane collagen previously shown to be upregulated in metastatic prostate cancer that has been used as a tissue and fluid biomarker for non-small cell lung cancer and prostate cancer. To determine whether collagen XXIII facilitates cancer cell metastasis in vivo and to establish a function for collagen XXIII in cancer progression, collagen XXIII knockdown cells were examined for alterations in in vivo metastasis as well as in vitro cell adhesion. In experimental and...

  10. Cellular Dichotomy Between Anchorage-Independent Growth Responses to bFGF and TA Reflects Molecular Switch in Commitment to Carcinogenesis

    Energy Technology Data Exchange (ETDEWEB)

    Waters, Katrina M.; Tan, Ruimin; Opresko, Lee K.; Quesenberry, Ryan D.; Bandyopadhyay, Somnath; Chrisler, William B.; Weber, Thomas J.

    2009-11-01

    We have investigated gene expression patterns underlying reversible and irreversible anchorage-independent growth (AIG) phenotypes to identify more sensitive markers of cell transformation for studies directed at interrogating carcinogenesis responses. In JB6 mouse epidermal cells, basic fibroblast growth factor (bFGF) induces an unusually efficient and reversible AIG response, relative to 12-O-tetradecanoyl phorbol-13-acetate (TPA)-induced AIG which is irreversible. The reversible and irreversible AIG phenotypes are characterized by largely non-overlapping global gene expression profiles. However, a subset of differentially expressed genes were identified as common to reversible and irreversible AIG phenotypes, including genes regulated in a reciprocal fashion. Hepatic leukemia factor (HLF) and D-site albumin promoter-binding protein (DBP) were increased in both bFGF and TPA soft agar colonies and selected for functional validation. Ectopic expression of human HLF and DBP in JB6 cells resulted in a marked increase in TPA- and bFGF-regulated AIG responses. HLF and DBP expression were increased in soft agar colonies arising from JB6 cells exposed to gamma radiation and in a human basal cell carcinoma tumor tissue, relative to paired non-tumor tissue. Subsequent biological network analysis suggests that many of the differentially expressed genes that are common to bFGF- and TPA-dependent AIG are regulated by c-Myc, SP-1 and HNF-4 transcription factors. Collectively, we have identified a potential molecular switch that mediates the transition from reversible to irreversible AIG.

  11. Downregulation of Akt1 Inhibits Anchorage-Independent Cell Growth and Induces Apoptosis in Cancer Cells

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    Xuesong Liu

    2001-01-01

    Full Text Available The serine/threonine kinases, Akti/PKBα, Akt2/PKBβ, and Akt3/PKBγ, play a critical role in preventing cancer cells from undergoing apoptosis. However, the function of individual Akt isoforms in the tumorigenicity of cancer cells is still not well defined. In the current study, we used an AM antisense oligonucleotide (AS to specifically downregulate Akti protein in both cancer and normal cells. Our data indicate that AM AS treatment inhibits the ability of MiaPaCa-2, H460, HCT-15, and HT1080 cells to grow in soft agar. The treatment also induces apoptosis in these cancer cells as demonstrated by FRCS analysis and a caspase activity assay. Conversely, Akti AS treatment has little effect on the cell growth and survival of normal human cells including normal human fibroblast (NHF, fibroblast from muscle (FBM, and mammary gland epithelial 184135 cells. In addition, AM AS specifically sensitizes cancer cells to typical chemotherapeutic agents. Thus, Akti is indispensable for maintaining the tumorigenicity of cancer cells. Inhibition of AM may provide a powerful sensitization agent for chemotherapy specifically in cancer cells.

  12. The G Protein-Coupled Estrogen Receptor-1, GPER-1, Promotes Fibrillogenesis via a Shc-Dependent Pathway Resulting in Anchorage-Independent Growth

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    Magruder, Hilary T.; Quinn, Jeffrey A.; Schwartzbauer, Jean E.; Reichner, Jonathan; Huang, Allan

    2016-01-01

    The G protein-coupled estrogen receptor-1, GPER-1, coordinates fibronectin (FN) matrix assembly and release of heparan-bound epidermal growth factor (HB-EGF). This mechanism of action results in the recruitment of FN-engaged integrin α5β1 to fibrillar adhesions and the formation of integrin α5β1-Shc adaptor protein complexes. Here, we show that GPER-1 stimulation of murine 4 T1 or human SKBR3 breast cancer cells with 17β-estradiol (E2β) promotes the formation of focal adhesions and actin stress fibers and results in increased cellular adhesion and haptotaxis on FN, but not collagen. These actions are also induced by the xenoestrogen, bisphenol A, and the estrogen receptor (ER) antagonist, ICI 182, 780, but not the inactive stereoisomer, 17α-estradiol (E2α). In addition, we show that GPER-1 stimulation of breast cancer cells allows for FN-dependent, anchorage-independent growth and FN fibril formation in “hanging drop” assays, indicating that these GPER-1-mediated actions occur independently of adhesion to solid substrata. Stable expression of Shc mutant Y317F lacking its primary tyrosyl phosphorylation site disrupts E2β-induced focal adhesion and actin stress fiber formation and abolishes E2β-enhanced haptotaxis on FN and anchorage-dependent growth. Collectively, these data demonstrate that E2β action via GPER-1 enhances cellular adhesivity and FN matrix assembly and allows for anchorage-independent growth, cellular events that may allow for cellular survival, and tumor progression. PMID:25096985

  13. Elevated p21-Activated Kinase 2 Activity Results in Anchorage-Independent Growth and Resistance to Anticancer Drug–Induced Cell Death

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    Jerry W. Marlin

    2009-03-01

    Full Text Available p21-Activated kinase 2 (PAK-2 seems to be a regulatory switch between cell survival and cell death signaling. We have shown previously that activation of full-length PAK-2 by Rac or Cdc42 stimulates cell survival, whereas caspase activation of PAK-2 to the proapoptotic PAK-2p34 fragment is involved in the cell death response. In this study, we present a role of elevated activity of full-length PAK-2 in anchorage-independent growth and resistance to anticancer drug–induced apoptosis of cancer cells. Hs578T human breast cancer cells that have low levels of PAK-2 activity were more sensitive to anticancer drug–induced apoptosis and showed higher levels of caspase activation of PAK-2 than MDA-MB435 and MCF-7 human breast cancer cells that have high levels of PAK-2 activity. To examine the role of elevated PAK-2 activity in breast cancer, we have introduced a conditionally active PAK-2 into Hs578T human breast cells. Conditional activation of PAK-2 causes loss of contact inhibition and anchorage-independent growth of Hs578T cells. Furthermore, conditional activation of PAK-2 suppresses activation of caspase 3, caspase activation of PAK-2, and apoptosis of Hs578T cells in response to the anticancer drug cisplatin. Our data suggest a novel mechanism by which full-length PAK-2 activity controls the apoptotic response by regulating levels of activated caspase 3 and thereby its own cleavage to the proapoptotic PAK-2p34 fragment. As a result, elevated PAK-2 activity interrupts the apoptotic response and thereby causes anchorage-independent survival and growth and resistance to anticancer drug–induced apoptosis.

  14. Exposure to bisphenol A correlates with early-onset prostate cancer and promotes centrosome amplification and anchorage-independent growth in vitro.

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    Pheruza Tarapore

    Full Text Available Human exposure to bisphenol A (BPA is ubiquitous. Animal studies found that BPA contributes to development of prostate cancer, but human data are scarce. Our study examined the association between urinary BPA levels and Prostate cancer and assessed the effects of BPA on induction of centrosome abnormalities as an underlying mechanism promoting prostate carcinogenesis. The study, involving 60 urology patients, found higher levels of urinary BPA (creatinine-adjusted in Prostate cancer patients (5.74 µg/g [95% CI; 2.63, 12.51] than in non-Prostate cancer patients (1.43 µg/g [95% CI; 0.70, 2.88] (p = 0.012. The difference was even more significant in patients <65 years old. A trend toward a negative association between urinary BPA and serum PSA was observed in Prostate cancer patients but not in non-Prostate cancer patients. In vitro studies examined centrosomal abnormalities, microtubule nucleation, and anchorage-independent growth in four Prostate cancer cell lines (LNCaP, C4-2, 22Rv1, PC-3 and two immortalized normal prostate epithelial cell lines (NPrEC and RWPE-1. Exposure to low doses (0.01-100 nM of BPA increased the percentage of cells with centrosome amplification two- to eight-fold. Dose responses either peaked or reached the plateaus with 0.1 nM BPA exposure. This low dose also promoted microtubule nucleation and regrowth at centrosomes in RWPE-1 and enhanced anchorage-independent growth in C4-2. These findings suggest that urinary BPA level is an independent prognostic marker in Prostate cancer and that BPA exposure may lower serum PSA levels in Prostate cancer patients. Moreover, disruption of the centrosome duplication cycle by low-dose BPA may contribute to neoplastic transformation of the prostate.

  15. Exposure to Bisphenol A Correlates with Early-Onset Prostate Cancer and Promotes Centrosome Amplification and Anchorage-Independent Growth In Vitro

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    Tarapore, Pheruza; Burke, Barbara; Bracken, Bruce; Ho, Shuk-Mei

    2014-01-01

    Human exposure to bisphenol A (BPA) is ubiquitous. Animal studies found that BPA contributes to development of prostate cancer, but human data are scarce. Our study examined the association between urinary BPA levels and Prostate cancer and assessed the effects of BPA on induction of centrosome abnormalities as an underlying mechanism promoting prostate carcinogenesis. The study, involving 60 urology patients, found higher levels of urinary BPA (creatinine-adjusted) in Prostate cancer patients (5.74 µg/g [95% CI; 2.63, 12.51]) than in non-Prostate cancer patients (1.43 µg/g [95% CI; 0.70, 2.88]) (p = 0.012). The difference was even more significant in patients <65 years old. A trend toward a negative association between urinary BPA and serum PSA was observed in Prostate cancer patients but not in non-Prostate cancer patients. In vitro studies examined centrosomal abnormalities, microtubule nucleation, and anchorage-independent growth in four Prostate cancer cell lines (LNCaP, C4-2, 22Rv1, PC-3) and two immortalized normal prostate epithelial cell lines (NPrEC and RWPE-1). Exposure to low doses (0.01–100 nM) of BPA increased the percentage of cells with centrosome amplification two- to eight-fold. Dose responses either peaked or reached the plateaus with 0.1 nM BPA exposure. This low dose also promoted microtubule nucleation and regrowth at centrosomes in RWPE-1 and enhanced anchorage-independent growth in C4-2. These findings suggest that urinary BPA level is an independent prognostic marker in Prostate cancer and that BPA exposure may lower serum PSA levels in Prostate cancer patients. Moreover, disruption of the centrosome duplication cycle by low-dose BPA may contribute to neoplastic transformation of the prostate. PMID:24594937

  16. Temporary Anchorage Device: An Epitome of Anchorage in Orthodontic Treatment

    Science.gov (United States)

    Nayak, US Krishna; Hegde, Amitha M; Jacob, Mary

    2011-01-01

    One of the most important phases of oral health is the form and function of the oral mechanism. Recently, pediatric dentists are concerned with the obvious esthetic disabilities and the pathologic implications of the malposed teeth. Interceptive and functional orthodontic treatment is playing a major role in these discrepancies. Anchorage is an important consideration in orthodontics, particularly if force is applied entirely to the teeth. For many years, clinicians have searched for a form of anchorage that does not rely on patient cooperation. During the last few decades, a wealth of new information has accumulated to such an extent that the present authors thought it appropriate to let these advances make an impact by suggesting a revised definition and classification of anchorage. This paper also gives a brief insight on evolution of anchorage and its application in pediatric dentistry.

  17. MicroRNA let-7b targets important cell cycle molecules in malignant melanoma cells and interferes with anchorage-independent growth

    Institute of Scientific and Technical Information of China (English)

    Julia Schultz; Peter Lorenz; Gerd Gross; Saleh Ibrahim; Manfred Kunz

    2008-01-01

    A microRNA expression screen was performed analyzing 157 different microRNAs in laser-microdissected tissues from benign melanocytic nevi (n=10) and primary malignant melanomas (n=10),using quantitative real-time PCR.Differential expression was found for 72 microRNAs.Members of the let-7 family of microRNAs were significantly downregulated in primary melanomas as compared with benign nevi,suggestive for a possible role of these molecules as tumor suppressors in malignant melanoma.Interestingly,similar findings had been described for lung and colon cancer.Overexpression of let-7b in melanoma cells in vitro downregulated the expression of cyclins D1,D3,and A,and cyclin-dependent kinase (Cdk) 4,all of which had been described to play a role in melanoma development.The effect oflet-7b on protein expression was due to targeting of 3'-untranslated regions (3'UTRs) of individual mRNAs,as exemplified by reporter gene analyses for cyclin DI.In line with its downmodulating effects on cell cycle regulators,let-7b inhibited cell cycle progression and anchorage-independent growth of melanoma cells.Taken together,these findings not only point to new regulatory mechanisms of early melanoma development,but also may open avenues for future targeted therapies of this tumor.

  18. Induction of anchorage-independent growth in primary human cells exposed to protons or HZE ions separately or in dual exposures.

    Science.gov (United States)

    Sutherland, B M; Cuomo, N C; Bennett, P V

    2005-10-01

    Travelers on space missions will be exposed to a complex radiation environment that includes protons and heavy charged particles. Since protons are present at much higher levels than are heavy ions, the most likely scenario for cellular radiation exposure will be proton exposure followed by a hit by a heavy ion. Although the effects of individual ion species on human cells are being investigated extensively, little is known about the effects of exposure to both radiation types. One useful measure of mammalian cell damage is induction of the ability to grow in a semi-solid agar medium highly inhibitory to the growth of normal human cells, termed neoplastic transformation. Using primary human cells, we evaluated induction of soft-agar growth and survival of cells exposed to protons only or to heavy charged particles (600 MeV/nucleon silicon) only as well as of cells exposed to protons followed after a 4-day interval by silicon ions. Both ions alone efficiently transformed the human cells to anchorage-independent growth. Initial experiments indicate that the dose responses for neoplastic transformation of cells exposed to protons and then after 4 days to silicon ions appear similar to that of cells exposed to silicon ions alone.

  19. Induction of anchorage-independent growth in primary human cells exposed to protons or HZE ions separately or in dual exposures.

    Science.gov (United States)

    Sutherland, B M; Cuomo, N C; Bennett, P V

    2005-10-01

    Travelers on space missions will be exposed to a complex radiation environment that includes protons and heavy charged particles. Since protons are present at much higher levels than are heavy ions, the most likely scenario for cellular radiation exposure will be proton exposure followed by a hit by a heavy ion. Although the effects of individual ion species on human cells are being investigated extensively, little is known about the effects of exposure to both radiation types. One useful measure of mammalian cell damage is induction of the ability to grow in a semi-solid agar medium highly inhibitory to the growth of normal human cells, termed neoplastic transformation. Using primary human cells, we evaluated induction of soft-agar growth and survival of cells exposed to protons only or to heavy charged particles (600 MeV/nucleon silicon) only as well as of cells exposed to protons followed after a 4-day interval by silicon ions. Both ions alone efficiently transformed the human cells to anchorage-independent growth. Initial experiments indicate that the dose responses for neoplastic transformation of cells exposed to protons and then after 4 days to silicon ions appear similar to that of cells exposed to silicon ions alone. PMID:16187755

  20. Down-regulation of Survivin by Antisense Oligonucleotides Increases Apoptosis, Inhibits Cytokinesis and Anchorage-Independent Growth

    Directory of Open Access Journals (Sweden)

    Jun Chen

    2000-05-01

    Full Text Available Survivin, a member of the inhibitor of apoptosis protein (IAP family, is detected in most common human cancers but not in adjacent normal cells. Previous studies suggest that survivin associates with the mitotic spindle and directly inhibits caspase activity. To further investigate the function of survivin, we used a survivin antisense (AS oligonucleotide to downregulate survivin expression in normal and cancer cells. We found that inhibition of survivin expression increased apoptosis and polyploidy while decreasing colony formation in soft agar. Immunohistochemistry showed that cells without survivin can initiate the cleavage furrow and contractile ring, but cannot complete cytokinesis, thus resulting in multinucleated cells. These findings indicate that survivin plays important roles in a late stage of cytokinesis, as well as in apoptosis.

  1. Anchorage-independent growth of pocket protein-deficient murine fibroblasts requires bypass of G2 arrest and can be accomplished by expression of TBX2

    NARCIS (Netherlands)

    Vormer, Tinke L; Foijer, Floris; Wielders, Camiel L C; te Riele, Hein

    2008-01-01

    Mouse embryonic fibroblasts (MEFs) deficient for pocket proteins (i.e., pRB/p107-, pRB/p130-, or pRB/p107/p130-deficient MEFs) have lost proper G(1) control and are refractory to Ras(V12)-induced senescence. However, pocket protein-deficient MEFs expressing Ras(V12) were unable to exhibit anchorage-

  2. Clathrin-independent endocytosis: mechanisms and function

    DEFF Research Database (Denmark)

    Sandvig, Kirsten; Pust, Sascha; Skotland, Tore;

    2011-01-01

    having several functions of their own. This article aims at providing a brief update on the importance of clathrin-independent endocytic mechanisms, how the processes are regulated differentially, for instance on the poles of polarized cells, and the challenges in studying them.......It is now about 20 years since we first wrote reviews about clathrin-independent endocytosis. The challenge at the time was to convince the reader about its existence. Then the suggestion came up that caveolae might be responsible for the uptake. However, clearly this could not be the case since...... a large fraction of the clathrin-independent uptake is dynamin-independent. Today, two decades later, the field has developed considerably. New techniques have enabled a detailed analysis of several clathrin-independent endocytic mechanisms, and caveolae have been found to be mostly stable structures...

  3. Inhibition of Anchorage-Independent Proliferation and G0/G1 Cell-Cycle Regulation in Human Colorectal Carcinoma Cells by 4,7-Dimethoxy-5-Methyl-l,3-Benzodioxole Isolated from the Fruiting Body of Antrodia camphorate

    Directory of Open Access Journals (Sweden)

    Hsiu-Man Lien

    2011-01-01

    Full Text Available In this study, 4,7-dimethoxy-5-methyl-l,3-benzodioxole (SY-1 was isolated from three different sources of dried fruiting bodies of Antrodia camphorate (AC. AC is a medicinal mushroom that grows on the inner heartwood wall of Cinnamomum kanehirai Hay (Lauraceae, an endemic species that is used in Chinese medicine for its anti-tumor and immunomodulatory properties. In this study, we demonstrated that SY-1 profoundly decreased the proliferation of human colon cancer cells (COLO 205 through G0/G1 cell-cycle arrest (50–150 μM and induction of apoptosis (>150 μM. Cell-cycle arrest induced by SY-1 was associated with a significant increase in levels of p53, p21/Cip1 and p27/Kip1, and a decrease in cyclins D1, D3 and A. In contrast, SY-1 treatment did not induce significant changes in G0/G1 phase cell-cycle regulatory proteins in normal human colonic epithelial cells (FHC. The cells were cultured in soft agar to evaluate anchorage-independent colony formation, and we found that the number of transformed colonies was significantly reduced in the SY-1-treated COLO 205 cells. These findings demonstrate for the first time that SY-1 inhibits human colon cancer cell proliferation through inhibition of cell growth and anchorage-independent colony formation in soft agar. However, the detailed mechanisms of these processes remain unclear and will require further investigation.

  4. TERRAIN, Anchorage, Alaska

    Data.gov (United States)

    Federal Emergency Management Agency, Department of Homeland Security — The Anchorage AOI consists of seven areas. These areas were to be collected to the 'highest' accuracy requirement. Ground Control is collected throughout the AOI...

  5. J domain independent functions of J proteins.

    Science.gov (United States)

    Ajit Tamadaddi, Chetana; Sahi, Chandan

    2016-07-01

    Heat shock proteins of 40 kDa (Hsp40s), also called J proteins, are obligate partners of Hsp70s. Via their highly conserved and functionally critical J domain, J proteins interact and modulate the activity of their Hsp70 partners. Mutations in the critical residues in the J domain often result in the null phenotype for the J protein in question. However, as more J proteins have been characterized, it is becoming increasingly clear that a significant number of J proteins do not "completely" rely on their J domains to carry out their cellular functions, as previously thought. In some cases, regions outside the highly conserved J domain have become more important making the J domain dispensable for some, if not for all functions of a J protein. This has profound effects on the evolution of such J proteins. Here we present selected examples of J proteins that perform J domain independent functions and discuss this in the context of evolution of J proteins with dispensable J domains and J-like proteins in eukaryotes.

  6. Alarm in Anchorage.

    Science.gov (United States)

    Campbell, Larry

    1991-01-01

    A possible sex scandal involving a teacher and a teenage student rocked the community in Anchorage, Alaska. Privacy and labor laws and union contract forbade board members from discussing the retirement deal that had been negotiated with the accused teacher. Hindsight suggests board members should have shared what information they could with the…

  7. Executive Dysfunction is Independently Associated with Reduced Functional Independence in Heart Failure

    Science.gov (United States)

    Alosco, Michael L.; Spitznagel, Mary Beth; Raz, Naftali; Cohen, Ronald; Sweet, Lawrence H.; Colbert, Lisa H.; Josephson, Richard; van Dulmen, Manfred; Hughes, Joel; Rosneck, Jim; Gunstad, John

    2016-01-01

    Aims and Objectives To examine the independent association between executive function with instrumental activities of daily living and health behaviors in older adults with heart failure. Background Executive function is an important contributor to functional independence as it consists of cognitive processes needed for decision-making, planning, organizing, and behavioral monitoring. Impairment in this domain is common in heart failure patients and associated with reduced performance of instrumental activities of daily living in many medical and neurological populations. However, the contribution of executive functions to functional independence and healthy lifestyle choices in heart failure patients has not been fully examined. Design Cross-sectional analyses. Methods 175 heart failure patients completed a neuropsychological battery and echocardiogram. Participants also completed the Lawton-Brody instrumental activities of daily living scale and reported current cigarette use. Results Hierarchical regressions revealed reduced executive function was independently associated with worse instrumental activity of daily living performance with a specific association for decreased ability to manage medications. Partial correlations showed executive dysfunction was associated with current cigarette use. Conclusions Our findings suggest that executive dysfunction is associated with poorer functional independence and contributes to unhealthy behaviors in heart failure. Future studies should examine whether heart failure patients benefit from formal organization schemae (i.e., pill organizers) to maintain independence. Relevance to Clinical Practice Screening of executive function in heart failure patients may provide key insight into their ability to perform daily tasks, including management of treatment recommendations. PMID:23650879

  8. Evaluation of functional (in)dependency in institutionalized elderly

    OpenAIRE

    Martins, Matilde; Vilela, Ana; Silva, Norberto

    2014-01-01

    Evaluation of daily life activities is considered an appropriate and legitimate tool for evaluating the degree of functional (in)dependency in the elderly. To evaluate the degree of functional (in)dependence in the elderly living in an long-term institution in the North of Portugal. Descriptive cross-sectional study conducted in a long-term residence in the North of Portugal. The sample was constituted by the totality of institutionalized elderly during the month of ...

  9. Functional independence measure in patients with intermittent claudication

    OpenAIRE

    Caroline Shihara de Assis; Letícia de Carvalho Batista; Nelson Wolosker; Antonio Eduardo Zerati; Rita de Cassia Gengo e Silva

    2015-01-01

    AbstractOBJECTIVEOf this study were to evaluate the functional independence of patients with intermittent claudication and to verify its association with sociodemographic and clinical variables, walking ability and physical activity level.METHODThis was a descriptive, exploratory, cross-sectional study with a quantitative approach. Fifty participants (66.4 years; 68% male) were recruited from Claudication Unit of a tertiary hospital. Functional Independence Measure were used to evaluate funct...

  10. Contributory factors for the functional independence of oldest old

    Directory of Open Access Journals (Sweden)

    Dâmarys Kohlbeck de Melo Neu Ribeiro

    2015-02-01

    Full Text Available OBJECTIVE To investigate the socioeconomic and clinical factors that contribute to the functional independence of the oldest old of a community. METHOD Cross-sectional quantitative study whose sample consisted of 214 elderly people registered in Basic Health Units. Data were collected through structured interviews and application of the Functional Independence Measure. We used descriptive statistics, and for association of the variables we used the Student t-test, ANOVA and Tukey's test for multiple comparisons. RESULTS The significant variables that contributed to the functional independence were remaining economically active, practicing physical and leisure activities, having a social life, eating fruits, vegetables and meat. The orientation to conduct these practices reduces the demand for care and help needed in everyday activities. CONCLUSION Maintaining independence is primordial to delay disability and presents itself as an excellent field of work for nursing.

  11. 77 FR 22489 - Special Anchorage Regulations, Newport Bay Harbor, CA

    Science.gov (United States)

    2012-04-16

    ... Anchorage Regulations, Newport Bay Harbor, CA'' in the Federal Register (76 FR 78185). We received no... businesses, not-for-profit organizations that are independently owned and operated and are not dominant in... aft moorings for recreational and small craft of such size and alignment as permitted by the...

  12. Correlation between demographic characteristics, cognitive functioning and functional independence in stroke patients

    OpenAIRE

    Arsić Slađana; Konstantinović Ljubica; Eminović Fadilj; Pavlović Dragan

    2016-01-01

    Introduction. It has been assumed that there is causality of the achieved level of functional independence with the degree of preservation of cognitive function in stroke patients. Demographic characteristics may be important for monitoring the achieved level of functional independence. Objective. The aim of this study was to examine the relationship of demographic characteristics and functional independence in regard to the level of cognitive impairment in ...

  13. Social Functions Decline with Age, Independently of Executive Functions

    OpenAIRE

    Gallacher, Tom

    2012-01-01

    Objectives- To determine whether old people are worse than young people at measures of social functions (SFs), and if any such effects can be explained by differences in executivefunctions performance (EFs). Design- A correlational design was used to determine group differences and within group correlations. Methods- 48 young participants (mean age=21) and 48 old participants (mean age=70.8) were administered The Mind in the Eyes (Baron Cohen et al. 2001) and The Awareness of ...

  14. [Bone-based anchorage failure].

    Science.gov (United States)

    Mer, Grégoria; Brezulier, Damien; Sorel, Olivier

    2016-03-01

    The aim of this article is to list the circumstances likely to give rise to failure of orthodontic temporary bone-supported anchorage and, hence, to attempt to define criteria for correct miniscrew usage. Our study was based on a review of the literature and analyses of clinical cases. Our findings show that, with a sound knowledge of the indications for screw selection and positioning and of the insertion protocols combined with a clear understanding of orthodontic mechanics, bone-based anchorage can henceforth provide orthodontists with an essential tool to enable formerly unachievable dental movements and to stabilize unwanted movements, thus making treatment both more reliable and more effective. PMID:27083224

  15. Bayesian Estimation of Conditional Independence Graphs Improves Functional Connectivity Estimates.

    Directory of Open Access Journals (Sweden)

    Max Hinne

    2015-11-01

    Full Text Available Functional connectivity concerns the correlated activity between neuronal populations in spatially segregated regions of the brain, which may be studied using functional magnetic resonance imaging (fMRI. This coupled activity is conveniently expressed using covariance, but this measure fails to distinguish between direct and indirect effects. A popular alternative that addresses this issue is partial correlation, which regresses out the signal of potentially confounding variables, resulting in a measure that reveals only direct connections. Importantly, provided the data are normally distributed, if two variables are conditionally independent given all other variables, their respective partial correlation is zero. In this paper, we propose a probabilistic generative model that allows us to estimate functional connectivity in terms of both partial correlations and a graph representing conditional independencies. Simulation results show that this methodology is able to outperform the graphical LASSO, which is the de facto standard for estimating partial correlations. Furthermore, we apply the model to estimate functional connectivity for twenty subjects using resting-state fMRI data. Results show that our model provides a richer representation of functional connectivity as compared to considering partial correlations alone. Finally, we demonstrate how our approach can be extended in several ways, for instance to achieve data fusion by informing the conditional independence graph with data from probabilistic tractography. As our Bayesian formulation of functional connectivity provides access to the posterior distribution instead of only to point estimates, we are able to quantify the uncertainty associated with our results. This reveals that while we are able to infer a clear backbone of connectivity in our empirical results, the data are not accurately described by simply looking at the mode of the distribution over connectivity. The implication

  16. Distribution-Independent Evolvability of Linear Threshold Functions

    CERN Document Server

    Feldman, Vitaly

    2011-01-01

    Valiant's (2007) model of evolvability models the evolutionary process of acquiring useful functionality as a restricted form of learning from random examples. Linear threshold functions and their various subclasses, such as conjunctions and decision lists, play a fundamental role in learning theory and hence their evolvability has been the primary focus of research on Valiant's framework (2007). One of the main open problems regarding the model is whether conjunctions are evolvable distribution-independently (Feldman and Valiant, 2008). We show that the answer is negative. Our proof is based on a new combinatorial parameter of a concept class that lower-bounds the complexity of learning from correlations. We contrast the lower bound with a proof that linear threshold functions having a non-negligible margin on the data points are evolvable distribution-independently via a simple mutation algorithm. Our algorithm relies on a non-linear loss function being used to select the hypotheses instead of 0-1 loss in V...

  17. Colour-independent partition functions in coloured vertex models

    Energy Technology Data Exchange (ETDEWEB)

    Foda, O., E-mail: omar.foda@unimelb.edu.au [Dept. of Mathematics and Statistics, University of Melbourne, Parkville, VIC 3010 (Australia); Wheeler, M., E-mail: mwheeler@lpthe.jussieu.fr [Laboratoire de Physique Théorique et Hautes Energies, CNRS UMR 7589 (France); Université Pierre et Marie Curie – Paris 6, 4 place Jussieu, 75252 Paris cedex 05 (France)

    2013-06-11

    We study lattice configurations related to S{sub n}, the scalar product of an off-shell state and an on-shell state in rational A{sub n} integrable vertex models, n∈{1,2}. The lattice lines are colourless and oriented. The state variables are n conserved colours that flow along the line orientations, but do not necessarily cover every bond in the lattice. Choosing boundary conditions such that the positions where the colours flow into the lattice are fixed, and where they flow out are summed over, we show that the partition functions of these configurations, with these boundary conditions, are n-independent. Our results extend to trigonometric A{sub n} models, and to all n. This n-independence explains, in vertex-model terms, results from recent studies of S{sub 2} (Caetano and Vieira, 2012, [1], Wheeler, (arXiv:1204.2089), [2]). Namely, 1.S{sub 2}, which depends on two sets of Bethe roots, {b_1} and {b_2}, and cannot (as far as we know) be expressed in single determinant form, degenerates in the limit {b_1}→∞, and/or {b_2}→∞, into a product of determinants, 2. Each of the latter determinants is an A{sub 1} vertex-model partition function.

  18. Colour-independent partition functions in coloured vertex models

    International Nuclear Information System (INIS)

    We study lattice configurations related to Sn, the scalar product of an off-shell state and an on-shell state in rational An integrable vertex models, n∈{1,2}. The lattice lines are colourless and oriented. The state variables are n conserved colours that flow along the line orientations, but do not necessarily cover every bond in the lattice. Choosing boundary conditions such that the positions where the colours flow into the lattice are fixed, and where they flow out are summed over, we show that the partition functions of these configurations, with these boundary conditions, are n-independent. Our results extend to trigonometric An models, and to all n. This n-independence explains, in vertex-model terms, results from recent studies of S2 (Caetano and Vieira, 2012, [1], Wheeler, (arXiv:1204.2089), [2]). Namely, 1.S2, which depends on two sets of Bethe roots, {b1} and {b2}, and cannot (as far as we know) be expressed in single determinant form, degenerates in the limit {b1}→∞, and/or {b2}→∞, into a product of determinants, 2. Each of the latter determinants is an A1 vertex-model partition function

  19. New concept of physiologic anchorage control

    OpenAIRE

    Tian-Min Xu

    2015-01-01

    Molar anchorage loss in extraction case is believed due to the reaction of mechanical force applied to retract anterior teeth. While it may be close to truth in adult patients, it is certainly not true in adolescents. Studies on molar growth show upper molar move forward as mandible growing forward, probably through intercuspation force. Hence, for adolescents, molar anchorage loss shall consist of two parts. One is from retraction force - mechanical anchorage loss; another from biologic forc...

  20. Colour-independent partition functions in coloured vertex models

    CERN Document Server

    Foda, O

    2013-01-01

    We study lattice configurations related to S_n, the scalar product of an off-shell state and an on-shell state in rational A_n integrable vertex models, n = {1, 2}. The lattice lines are colourless and oriented. The state variables are n conserved colours that flow along the line orientations, but do not necessarily cover every bond in the lattice. Choosing boundary conditions such that the positions where the colours flow into the lattice are fixed, and where they flow out are summed over, we show that the partition functions of these configurations, with these boundary conditions, are n-independent. Our results extend to trigonometric A_n models, and to all n. This n-independence explains, in vertex-model terms, results from recent studies of S_2 [1, 2]. Namely, 1. S_2 which depends on two sets of Bethe roots, b_1 and b_2, and cannot (as far as we know) be expressed in single determinant form, degenerates in the limit b_1 -> infinity, and/or b_2 -> infinity, into a product of determinants, 2. Each of the la...

  1. Drosha regulates gene expression independently of RNA cleavage function

    DEFF Research Database (Denmark)

    Gromak, Natalia; Dienstbier, Martin; Macias, Sara;

    2013-01-01

    Drosha is the main RNase III-like enzyme involved in the process of microRNA (miRNA) biogenesis in the nucleus. Using whole-genome ChIP-on-chip analysis, we demonstrate that, in addition to miRNA sequences, Drosha specifically binds promoter-proximal regions of many human genes in a transcription......-terminal protein-interaction domain, which associates with the RNA-binding protein CBP80 and RNA Polymerase II. Consequently, we uncover a previously unsuspected RNA cleavage-independent function of Drosha in the regulation of human gene expression.......Drosha is the main RNase III-like enzyme involved in the process of microRNA (miRNA) biogenesis in the nucleus. Using whole-genome ChIP-on-chip analysis, we demonstrate that, in addition to miRNA sequences, Drosha specifically binds promoter-proximal regions of many human genes in a transcription......-dependent manner. This binding is not associated with miRNA production or RNA cleavage. Drosha knockdown in HeLa cells downregulated nascent gene transcription, resulting in a reduction of polyadenylated mRNA produced from these gene regions. Furthermore, we show that this function of Drosha is dependent on its N...

  2. Correlation between demographic characteristics, cognitive functioning and functional independence in stroke patients

    Directory of Open Access Journals (Sweden)

    Arsić Slađana

    2016-01-01

    Full Text Available Introduction. It has been assumed that there is causality of the achieved level of functional independence with the degree of preservation of cognitive function in stroke patients. Demographic characteristics may be important for monitoring the achieved level of functional independence. Objective. The aim of this study was to examine the relationship of demographic characteristics and functional independence in regard to the level of cognitive impairment in stroke patients. Methods. The study included 50 stroke patients after rehabilitation, as well as age- and gender-matched 50 subjects selected randomly, according to the demographic characteristics of the studied sample, who in their medical history had no neurological disorders. For the assessment of functional independence, the Functional Independence Measure (FIM test was used. The general cognition was estimated by the Mini-Mental State Examination (MMSE test. The statistical analyses included the Mann-Whitney test, for two independent samples, measures of canonical correlation, and χ2 test. Results. There was a statistically significant difference between the groups in relation to risk factors, hypertension and diabetes mellitus type II (p<0.001; There was a statistically significant difference within the groups in relation to the cognitive impairment in all the examined demographic characteristics (p<0.001; the differences within the groups in relation to the cognitive impairment are present on all subscales of the FIM test (p<0.05; the differences within the groups in relation to handedness, hemiparesis, show that mild cognitive impairment is more common among left hemiparesis, while a more severe one is more common among right-sided hemiparesis (p<0.05; More severe cognitive impairment is common among women, the elderly and in persons with lower education (p<0.05. Conclusion. By prevention of risk factors, and prevention of possible cognitive impairment, consequences of stroke can be

  3. Smooth muscle cells largely develop independently of functional hemogenic endothelium

    Directory of Open Access Journals (Sweden)

    Monika Stefanska

    2014-01-01

    Full Text Available Vascular smooth muscle cells represent a major component of the cardiovascular system. In vitro studies have shown that FLK1+ cells derived from embryonic stem (ES cells can differentiate into both endothelial and smooth muscle cells. These FLK1+ cells also contain a mesodermal precursor, the hemangioblast, able to produce endothelial, blood and smooth muscle cells. The generation of blood precursors from the hemangioblast was recently shown to occur through a transient cell population of specialised endothelium, a hemogenic endothelium. To date, the lineage relationship between this cell population and smooth muscle cell progenitors has not been investigated. In this study, we generated a reporter ES cell line in which expression of the fluorescent protein H2B-VENUS is driven by the α-smooth muscle actin (α-SMA regulatory sequences. We demonstrated that this reporter cell line efficiently trace smooth muscle development during ES cell differentiation. Although some smooth muscle cells are associated with broad endothelial development, we established that smooth muscle cells are mostly generated independently from a specialised functional hemogenic endothelium. This study provides new and important insights into hematopoietic and vascular development, which may help in driving further progress towards the development of bioengineered vascular grafts for regenerative medicine.

  4. Orthodontic Anchorage Implants: State-of-the-art

    DEFF Research Database (Denmark)

    Melsen, Birte

    2007-01-01

    The limits of an orthodontic treatment are often set by the lack of suitable anchorage. The mini-implant is used where conventional anchorage cannot be applied and not as a replacement for conventional anchorage. In patients with lack of teeth and reduced periodontium, skeletal anchorage allows...... will improve the reconstruction possibilities. The mini-implant has widened the orthodontic possibilities....

  5. Algebraic Independence of Certain Values of Exponential Function

    Institute of Scientific and Technical Information of China (English)

    Yao Chen ZHU

    2006-01-01

    The algebraic independence of eθ1,...,eθs is proved, where θ1,...,θs are certain gap series or power series of algebraic numbers, or certain transcendental continued fractions with algebraic elements.

  6. 76 FR 76295 - Anchorage Regulations; Wells, ME

    Science.gov (United States)

    2011-12-07

    ... rulemaking (NPRM) entitled ``Anchorage Regulations; Wells, Maine'' in the Federal Register (76 FR 52599). We..., Maine. All coordinates referenced use datum: NAD 83. Dated: November 21, 2011. Daniel A. Neptun,...

  7. A role for Mfb1p in region-specific anchorage of high-functioning mitochondria and lifespan in Saccharomyces cerevisiae

    OpenAIRE

    Pernice, Wolfgang M.; Vevea, Jason D.; Pon, Liza A.

    2016-01-01

    Previous studies indicate that replicative lifespan in daughter cells of Sacchraromyces cerevisiae depends on the preferential inheritance of young, high-functioning mitochondria. We report here that mitochondria are functionally segregated even within single mother cells in S. cerevisiae. A high-functioning population of mitochondria accumulates at the tip of the mother cell distal to the bud. We find that the mitochondrial F-box protein (Mfb1p) localizes to mitochondria in the mother tip an...

  8. Creators' Intentions Bias Judgments of Function Independently from Causal Inferences

    Science.gov (United States)

    Chaigneau, Sergio E.; Castillo, Ramon D.; Martinez, Luis

    2008-01-01

    Participants learned about novel artifacts that were created for function X, but later used for function Y. When asked to rate the extent to which X and Y were a given artifact's function, participants consistently rated X higher than Y. In Experiments 1 and 2, participants were also asked to rate artifacts' efficiency to perform X and Y. This…

  9. How Disabling Are Pediatric Burns? Functional Independence in Dutch Pediatric Patients with Burns

    Science.gov (United States)

    Disseldorp, Laurien M.; Niemeijer, Anuschka S.; Van Baar, Margriet E.; Reinders-Messelink, Heleen A.; Mouton, Leonora J.; Nieuwenhuis, Marianne K.

    2013-01-01

    Although the attention for functional outcomes after burn injury has grown over the past decades, little is known about functional independence in performing activities of daily living in children after burn injury. Therefore, in this prospective cohort study functional independence was measured by burn care professionals with the WeeFIM[R]…

  10. Evaluation of functional independence after discharge from the intensive care unit

    OpenAIRE

    Curzel, Juliane; Forgiarini Junior, Luiz Alberto; Rieder, Marcelo de Mello

    2013-01-01

    Objective 1) To evaluate the functional independence measures immediately after discharge from an intensive care unit and to compare these values with the FIMs 30 days after that period. 2) To evaluate the possible associated risk factors. Methods The present investigation was a prospective cohort study that included individuals who were discharged from the intensive care unit and underwent physiotherapy in the unit. Functional independence was evaluated using the functional independence meas...

  11. Correlation between functional independence and quality of life of patients with amyotrophic lateral sclerosis

    OpenAIRE

    Nathalia Priscilla Oliveira Silva; Lizianne Juline do Nascimento e Silva Martins; Thaiana Barbosa Ferreira; Fabrícia Azevedo da Costa Cavalcanti

    2014-01-01

    Functional independence and quality of life are impacted by amyotrophic lateral sclerosis (ALS), a degenerative and progressive disease. The aim of this study was to investigate the functional independence and quality of life of patients with ALS in the municipality of Natal, Rio Grande do Norte state, Brazil. This is a cross-sectional observational study conducted with 24 patients. The Amyotrophic Lateral Sclerosis Assessment Questionnaire (ALSAQ-40/BR) and the Functional Independence Measur...

  12. GAP-independent functions of DLC1 in metastasis.

    Science.gov (United States)

    Barras, David; Widmann, Christian

    2014-03-01

    Metastases are responsible for most cancer-related deaths. One of the hallmarks of metastatic cells is increased motility and migration through extracellular matrixes. These processes rely on specific small GTPases, in particular those of the Rho family. Deleted in liver cancer-1 (DLC1) is a tumor suppressor that bears a RhoGAP activity. This protein is lost in most cancers, allowing malignant cells to proliferate and disseminate in a Rho-dependent manner. However, DLC1 is also a scaffold protein involved in alternative pathways leading to tumor and metastasis suppressor activities. Recently, substantial information has been gathered on these mechanisms and this review is aiming at describing the potential and known alternative GAP-independent mechanisms allowing DLC1 to impair migration, invasion, and metastasis formation.

  13. On English Independent Structure Syntactic and Pragmatic Functions

    Institute of Scientific and Technical Information of China (English)

    刘玲玲

    2014-01-01

    Many western linguists began to pay much attention to small clause since 1980s. There is little specialized research on the structure of small clause,and the related explanations in the grammar book are about its surface features instead of its internal syntactic structure. Syntax as an independent science has not finished explaining all the linguistic phenomena,for the structure of small clause is a disputable topic which needs explanation and research. Last but not least,I would also like to thank with all my heart my parents for their love, understanding,support,and encouragement throughout my writing of the thesis and the complete two and a half years of graduate study. It was he who was always ready to solve my problems in studies and research. It was he who offered me suggestions and references for this thesis. And it was he who helped me in reviewing the whole thesis. My words are too weak to express my gratefulness to him.

  14. Independent contribution of individual white matter pathways to language function in pediatric epilepsy patients

    Directory of Open Access Journals (Sweden)

    Michael J. Paldino, M.D.

    2014-01-01

    Conclusions: Scalar metrics derived from the left uncinate, inferior fronto-occipital, and arcuate fasciculi were independently associated with language function. These results support the importance of these pathways in human language function in patients with MCDs.

  15. Is skeletal anchorage changing the limit of orthodontics?

    DEFF Research Database (Denmark)

    Melsen, Birte

    2007-01-01

    The limits for orthodontic treatment are often set by the lack of suitable anchorage. The mini-implant is used where conventional anchorage cannot be applied; not as a replacement for conventional anchorage. In patients with lack of teeth and reduced periodontium, skeletal anchorage allows...... and can be loaded immediately. The course will be addressed the following topics: Are the mini-implants replacing conventional anchorage? Why are orthodontic mini-implants necessary? The development of the skeletal anchorage systems The biological basis for the skeletal anchorage systems...... The characteristics of the different skeletal anchorage systems The insertion procedure The indications for the use of orthodontic mini-implants Treatment planning in relation to the use of mini-implants Case presentations...

  16. Friction does not increase anchorage loading.

    Science.gov (United States)

    Southard, Thomas E; Marshall, Steve D; Grosland, Nicole M

    2007-03-01

    Conventional wisdom suggests that orthodontists must apply added force to overcome friction during canine retraction (sliding mechanics), the result of which can be increased anchorage loading and anchorage loss. However, for a frictional force to be exerted mesially by the archwire against a canine during retraction, the archwire must be compressed between the canine and the anchor molar, and an equal but opposite force must be applied distally against the molar by the archwire. In other words, the frictional force that reduces the force of retraction on the canine must also reduce the protraction force on the molar. Emphasis on employing reduced-friction (eg, self-ligating) brackets during sliding mechanics to prevent added posterior anchorage loading is unwarranted and based more on bracket salesmanship than on orthodontic biomechanics. PMID:17346599

  17. Paradigm shifts in orthodontic treatment with mini-implant anchorage

    Directory of Open Access Journals (Sweden)

    Johnny Joung-Lin Liaw

    2015-01-01

    Full Text Available After the applications of mini-implant anchorage, the envelope of orthodontic treatment was expanded and some treatment modes were changed because of more predictable tooth movement with empowered anchorage. The author tried to share his experience of TADs applications for clarifying the paradigm shifts of orthodontic treatment assisted with the mini-implant anchorage.

  18. The influence of demographic, environmental and physical factors on functional independence post stroke

    Directory of Open Access Journals (Sweden)

    M.V. Mamabolo

    2008-02-01

    Full Text Available Purpose: The magnitude of disability observed in strokesurvivors is believed to be dependent in part, on the severity of neurological deficits incurred. A s important but less well understood, is thecontribution of demographic, physical and environmental factors. The objective of this study was to establish what demographic, environmentaland physical factors influence functional independence post stroke. Method: Convenience sampling was used in the selection of subjects from four stroke outpatient public health facilities in the Gauteng Province of South Africa. The data were collected using a structured questionnaire. The analytical tools used included descriptive statistics to measure percentages and cross tabulations to measure the level of associations between functional independence and some of the demographic factors. The Barthel Index was computed to establish the degree of functional independence. Finally the influence of factors on functional independence was investigated using bivariate logistic regressions.Results: The results showed that younger patients (18 - 34 yrs may have a higher likelihood of functional independence compared to older patients at the time of discharge from hospital (18 - 34 years: Odds Ratio = 1. Patients without helpers were more likely to be functionally independent than those with a helper (p = 0.03. Involvement in household activities (p = 0.01, participation in community activities (p = 0.02 and bowel and bladder continence (p = 0.003 and p = 0.04 improved the likelihood of functional independence.Conclusion and im plications: Factors that influence functional independence post stroke are: age, bowel and bladder continence, the presence of a caregiver, participation in household and community activities. It is also of value to encourage patients to participate in household and community activities post stroke as well as being less dependent on helpers in an effort to attain functional independence post

  19. Biological reactions to temporary anchorage devices

    NARCIS (Netherlands)

    G. Wu; J. Guo; H. Hu; V. Everts

    2015-01-01

    This chapter reviews the current knowledge of the biological reactions to temporary anchorage devices (TADs) and the factors that affect a successful application of TADs. In addition to clinical investigations, finite element analysis (FEA) is also extensively used to analyze the factors on the mech

  20. Measuring the democratic anchorage of governance networks

    DEFF Research Database (Denmark)

    Fotel, Trine; Sørensen, Eva; Torfing, Jacob

    model helps to assess the democratic performance of specific governance networks and to gain knowledge about the critical factors that determine their degree of democratic anchorage. Such knowledge is crucial for developing proactive strategies for enhancing the democratic performance of specific...

  1. Functional Outcomes and Consumer Satisfaction in the Independent Living Program for Older Individuals Who Are Blind

    Science.gov (United States)

    Moore, J. Elton; Steinman, Bernard A.; Giesen, J. Martin; Frank, John J.

    2006-01-01

    This study of a national sample of elders served by the Independent Living Program for Older Individuals Who Are Blind found that, overall, they were highly satisfied with the quality and timeliness of services and help in achieving independent living goals. A slight improvement was found in their perceptions of functional outcomes from 1999 to…

  2. The Functional Independence of Mands and Tacts: Has It Been Demonstrated Empirically?

    Science.gov (United States)

    Gamba, Jonas; Goyos, Celso; Petursdottir, Anna Ingeborg

    2015-01-01

    Recently, there has been a proliferation of research on the functional independence of two of Skinner's (1957) verbal operants, the mand, and the tact. This research has produced highly variable results. In this article, we provide a critical review of the literature on mand-tact independence, a literature that has implications for both theory and…

  3. Impulse-response function of splanchnic circulation with model-independent constraints: theory and experimental validation

    DEFF Research Database (Denmark)

    Munk, O L; Keiding, S; Bass, L

    2003-01-01

    from constraining the regression fits by parameters estimated independently. This is strong evidence for the physiological relevance of the impulse-response function, which includes asymptotically, and thereby justifies kinetically, a useful and simple power law. Similarity between our parameter...

  4. Moderate deviations and functional limits for random processes with stationary and independent increments

    Institute of Scientific and Technical Information of China (English)

    GAO; Fuqing

    2006-01-01

    We first give a functional moderate deviation principle for random processes with stationary and independent increments under the Ledoux's condition.Then we apply the result to laws of the iterated logarithm.

  5. Self-perceived health in functionally independent older people: associated factors

    OpenAIRE

    Machón, Mónica; Vergara, Itziar; Dorronsoro, Miren; Vrotsou, Kalliopi; Larrañaga, Isabel

    2016-01-01

    Background Self-perceived health (SPH) is a powerful indicator of the health status of elderly people. This issue has been widely studied in oldest populations considering altogether functionally independent and dependent individuals. The objective of this study was to describe SPH and to identify the main factors that have an impact on SPH in a sample of functionally independent community-dwelling older adults. Methods For this cross-sectional study, face-to-face interviews were carried out ...

  6. Visual contrast sensitivity in patients with impairment of functional independence after stroke

    OpenAIRE

    dos Santos Natanael; Andrade Suellen

    2012-01-01

    Abstract Background Stroke has been considered a serious public health problem in many countries, accounting for complex disorders involving perception, such as visual, cognitive and functional deficits. The impact of stroke on the visual perception of individuals with impairments in functional independence was investigated. Methods We measured changes in functional independence and visual function in 40 patients with stroke (M = 52.3, SD = 0.65) and 10 controls (M = 52.5, SD = 0.66). The pat...

  7. Possible situation for gauge independence of wave-function renormalization constants in gauge-field theories

    International Nuclear Information System (INIS)

    Possibility of gauge independence of wave-function renormalization constants is studied on the basis of gauge-field theories with gauge covariance. By use of the expression for the double-pole type propagator D tilde sub( f)(x), exploited by Zwanziger, it is asserted that D tilde sub( f)(0) can be consistently taken zero. As a consequence, all renormalization constants become gauge independent, contrary to the conventional understanding. (author)

  8. Calibrating Independence Goals and Partner Support: Couples Adjust to Functional Limitations after Tumor Surgery

    OpenAIRE

    Knoll, N; Wiedemann, AU; Schrader, M; Felber, J.; Burkert, S; Daig, I; Heckhausen, J

    2015-01-01

    © 2015 The International Association of Applied Psychology. When patients recover from disease-related functional limitations, support received from partners may not always match patients' changing independence goals. The lines of defense (LoD) model proposes a hierarchy of independence goals (LoDs), ranging from minimising discomfort by disengagement (lowest LoD) to protection of self-reliance (highest LoD). Prostate cancer patients' LoDs were examined as moderators of the association betwee...

  9. Functional independence and health-related functional status following spinal cord injury : a prospective study of the association with physical capacity

    NARCIS (Netherlands)

    Haisma, Janneke A.; Post, Marcel W.; van der Woude, Lucas H.; Stam, Henk J.; Bergen, Michael P.; Sluis, Tebbe A.; van den Berg-Emons, Hendrika J.; Bussmann, Johannes B.

    2008-01-01

    Objective: To determine changes in functional independence following spinal cord injury and to evaluate the association between functional independence and physical capacity. Design: Multi-centre prospective cohort study. Subjects: Patients with spinal cord injury admitted for initial rehabilitation

  10. Detection of independent functional networks during music listening using electroencephalogram and sLORETA-ICA.

    Science.gov (United States)

    Jäncke, Lutz; Alahmadi, Nsreen

    2016-04-13

    The measurement of brain activation during music listening is a topic that is attracting increased attention from many researchers. Because of their high spatial accuracy, functional MRI measurements are often used for measuring brain activation in the context of music listening. However, this technique faces the issues of contaminating scanner noise and an uncomfortable experimental environment. Electroencephalogram (EEG), however, is a neural registration technique that allows the measurement of neurophysiological activation in silent and more comfortable experimental environments. Thus, it is optimal for recording brain activations during pleasant music stimulation. Using a new mathematical approach to calculate intracortical independent components (sLORETA-IC) on the basis of scalp-recorded EEG, we identified specific intracortical independent components during listening of a musical piece and scales, which differ substantially from intracortical independent components calculated from the resting state EEG. Most intracortical independent components are located bilaterally in perisylvian brain areas known to be involved in auditory processing and specifically in music perception. Some intracortical independent components differ between the music and scale listening conditions. The most prominent difference is found in the anterior part of the perisylvian brain region, with stronger activations seen in the left-sided anterior perisylvian regions during music listening, most likely indicating semantic processing during music listening. A further finding is that the intracortical independent components obtained for the music and scale listening are most prominent in higher frequency bands (e.g. beta-2 and beta-3), whereas the resting state intracortical independent components are active in lower frequency bands (alpha-1 and theta). This new technique for calculating intracortical independent components is able to differentiate independent neural networks associated

  11. Assessing the Employment and Independence of People Who Are Deaf and Low Functioning.

    Science.gov (United States)

    Wheeler-Scruggs, Kathy

    2002-01-01

    Interviews with 23 former or current consumers of vocational rehabilitation services found not all were employed but the majority lived independently. Employees were happy with their jobs. However, workplace accommodations were virtually nonexistent. Suggestions are provided on areas in which people who are deaf and low functioning may need…

  12. Investigation of Cool and Hot Executive Function in ODD/CD Independently of ADHD

    Science.gov (United States)

    Hobson, Christopher W.; Scott, Stephen; Rubia, Katya

    2011-01-01

    Background: Children with oppositional defiant disorder/conduct disorder (ODD/CD) have shown deficits in "cool" abstract-cognitive, and "hot" reward-related executive function (EF) tasks. However, it is currently unclear to what extent ODD/CD is associated with neuropsychological deficits, independently of attention deficit hyperactivity disorder…

  13. IMPROVING FUNCTIONAL INDEPENDENCE OF PATIENTS WITH MULTIPLE SCLEROSIS BY PHYSICAL THERAPY AND OCCUPATIONAL THERAPY

    OpenAIRE

    Ana-Maria Ticărat

    2011-01-01

    Introduction. Patients with multiple sclerosis can have a normal life despite of their real or possible disability and of the progressive nature of it. Scope. Patients who follow physical therapy and occupational therapy will have an increased quality of life and a greater functional independence.Methods. The randomized study was made on 7 patients with multiple sclerosis, from Oradea Day Centre, 3 times/week, ages between 35 – 55 years, functional level between mild and sever. Assessment an...

  14. Anchorage of Main Reinforcement in Lightweight Aggregate Concrete Beams

    DEFF Research Database (Denmark)

    Larsen, Henning

    1999-01-01

    The paper deals with the anchorage of reinforcement bars at end supports in beam component made of lightweight aggregate concrete with open structure.......The paper deals with the anchorage of reinforcement bars at end supports in beam component made of lightweight aggregate concrete with open structure....

  15. 75 FR 76275 - Anchorage Regulations; Long Island Sound

    Science.gov (United States)

    2010-12-08

    ...In this rule the Coast Guard establishes seven anchorage grounds in Long Island Sound. These anchorages are located in Connecticut and New York State waters. This action is necessary to aid in facilitating the safe and secure anchoring and transiting of vessels, particularly deep draft vessels, transiting Long Island Sound or awaiting entry to a port or facility in New York and...

  16. Mechanical anchorage of FRP tendons – A literature review

    DEFF Research Database (Denmark)

    Schmidt, Jacob W.; Bennitz, Anders; Täljsten, Björn;

    2012-01-01

    anchorage systems for use with Aramid, Glass and Carbon FRP tendons have been proposed over the last two decades. Each system is usually tailored to a particular type of tendon. This paper presents a brief overview of bonded anchorage applications while the primary literature review discusses three methods...

  17. FRP tendon anchorage in post-tensioned concrete structures

    DEFF Research Database (Denmark)

    Schmidt, Jacob Wittrup; Täljsten, Björn; Bennitz, Anders;

    2008-01-01

    effective Young´s modulus and the high stress capacity in the linear elastic range of the material. The use of external tendons increases the requirements on the anchorage systems. This is in particular important when using un-bonded tendon systems, where the anchorage and deviators are the only force...

  18. Quadratic and rate-independent limits for a large-deviations functional

    Science.gov (United States)

    Bonaschi, Giovanni A.; Peletier, Mark A.

    2016-07-01

    We construct a stochastic model showing the relationship between noise, gradient flows and rate-independent systems. The model consists of a one-dimensional birth-death process on a lattice, with rates derived from Kramers' law as an approximation of a Brownian motion on a wiggly energy landscape. Taking various limits, we show how to obtain a whole family of generalized gradient flows, ranging from quadratic to rate-independent ones, connected via ` L log L' gradient flows. This is achieved via Mosco-convergence of the renormalized large-deviations rate functional of the stochastic process.

  19. Slimmer women's waist is associated with better erectile function in men independent of age.

    Science.gov (United States)

    Brody, Stuart; Weiss, Petr

    2013-10-01

    Previous research has indicated that men generally rate slimmer women as more sexually attractive, consistent with the increased morbidity risks associated with even mild abdominal adiposity. To assess the association of women's waist size with a more tangible measure of perceived sexual attractiveness (as well as reward value for both sexes), we examined the association of women's age and waist circumference with an index of men's erectile function (IIEF-5 scores), frequency of penile-vaginal intercourse (PVI), and sexual satisfaction in a representative sample of Czechs (699 men and 715 women) aged 35-65 years. Multivariate analyses indicated that better erectile function scores were independently associated with younger age of self and partner and women's slimmer waist. PVI frequency was independently associated with women's younger age and women's slimmer waist. Sexual satisfaction was independently associated with men's younger age and slimmer waist for both sexes. Better erectile function, greater PVI frequency, and greater sexual satisfaction were associated with women's slimmer waist, independently of both sexes' ages. Possible reasons for the waist effects were discussed, including women's abdominal body fat decreasing their own desire through neurohormonal mechanisms and decreasing their partner's desire through evolutionarily-related decreased sexual attractiveness. PMID:23264164

  20. IMPROVING FUNCTIONAL INDEPENDENCE OF PATIENTS WITH MULTIPLE SCLEROSIS BY PHYSICAL THERAPY AND OCCUPATIONAL THERAPY

    Directory of Open Access Journals (Sweden)

    Ana-Maria Ticărat

    2011-06-01

    Full Text Available Introduction. Patients with multiple sclerosis can have a normal life despite of their real or possible disability and of the progressive nature of it. Scope. Patients who follow physical therapy and occupational therapy will have an increased quality of life and a greater functional independence.Methods. The randomized study was made on 7 patients with multiple sclerosis, from Oradea Day Centre, 3 times/week, ages between 35 – 55 years, functional level between mild and sever. Assessment and rehabilitation methods: inspection, BARTHEL Index. Frenkel method, brething exercises, weights exercises, gait exercises, writind exercises and games were used in the rehabilitation process. Group therapies: sociotherapy, arttherapy, music therapy. Results analysis consisted of the comparison of baseline and final means.Results. By analizing baseline and final means for Barthel Index for each functon separately, it was shown a mild improvement of functional independence for almost assessed functions, with at least 1-1,5 points.Conclusions. Persons with multiple sclerosis who follow physical therapy and occupational therapy presents a better functional independence after the treatment.

  1. Functional independence in resting-state connectivity facilitates higher-order cognition.

    Science.gov (United States)

    James, G Andrew; Kearney-Ramos, Tonisha E; Young, Jonathan A; Kilts, Clinton D; Gess, Jennifer L; Fausett, Jennifer S

    2016-06-01

    Growing evidence suggests that intrinsic functional connectivity (i.e. highly structured patterns of communication between brain regions during wakeful rest) may encode cognitive ability. However, the generalizability of these findings is limited by between-study differences in statistical methodology and cognitive domains evaluated. To address this barrier, we evaluated resting-state neural representations of multiple cognitive domains within a relatively large normative adult sample. Forty-four participants (mean(sd) age=31(10) years; 18 male and 26 female) completed a resting-state functional MRI scan and neuropsychological assessments spanning motor, visuospatial, language, learning, memory, attention, working memory, and executive function performance. Robust linear regression related cognitive performance to resting-state connectivity among 200 a priori determined functional regions of interest (ROIs). Only higher-order cognitions (such as learning and executive function) demonstrated significant relationships between brain function and behavior. Additionally, all significant relationships were negative - characterized by moderately positive correlations among low performers and weak to moderately negative correlations among high performers. These findings suggest that functional independence among brain regions at rest facilitates cognitive performance. Our interpretation is consistent with graph theoretic analyses which represent the brain as independent functional nodes that undergo dynamic reorganization with task demand. Future work will build upon these findings by evaluating domain-specific variance in resting-state neural representations of cognitive impairment among patient populations. PMID:27105037

  2. Field dependence-independence (FDI) cognitive style: an analysis of attentional functioning.

    Science.gov (United States)

    Guisande, M Adelina; Páramo, M Fernanda; Tinajero, Carolina; Almeida, Leandro S

    2007-11-01

    Previous research has indicated that field-dependent children display poorer performance than field-independent children in almost all academic subjects and cognitive tasks. However, the processes underlying this poorer performance remain unclear. The present study aimed to assess whether children with different FDI cognitive styles show differences in performance of tasks measuring aspects of attentional functioning. Specifically, 149 children aged 8 - 11 years were classified according to FDI cognitive style (field-dependent, intermediate, or field-independent), and to storage capacity (Digits Forward Test), verbal working memory (Digits Backward Test), capacity to focus, shift, and maintain attention (Digit Symbol Test), and capacity for sustained attention (Visual Search and Attention Test). Field-independent children displayed better performance than intermediate and field-dependent children on all tests except the Digits Forward Test. Theoretical and practical implications of these results are discussed.

  3. [Zygomatic anchorage concept in full edentulism].

    Science.gov (United States)

    Malevez, C

    2012-09-01

    The zygoma anchorage concept for severe atrophy of the maxilla was developed by Brånemark in the 1980s. It concerned patients having undergone maxillectomy as well as patients with edentulous upper jaws and mild to severe maxillary atrophy. The concept was created and clinically developed in 1997, by placing two zygomatic implants and four standard ones to compensate sub-sinus bone resorption. It was applied later to patients presenting extremely severe maxillary atrophy, using the "zygoma quad" concept with the placement of four zygomatic implants. Zygoma anchorage is reliable in terms of osseo-integration and biomechanical support of screwed prostheses, according to frequently published data (overall survival rate ranging between 91 and 100%). The increasing practice of immediate loading makes it the treatment of choice for oral rehabilitation. It decreases surgical invasiveness and the delay between surgery and oral rehabilitation. It allows a quick social and professional reinsertion and increases the comfort and quality of life for patients with fully edentulous maxilla with mild, moderate, or severe atrophy. PMID:22920891

  4. 78 FR 3326 - Safety Zone Within the Lower Portion of Anchorage #9, Mantua Creek Anchorage; Paulsboro, NJ

    Science.gov (United States)

    2013-01-16

    ... 20590, between 9 a.m. and 5 p.m., Monday through Friday, except Federal holidays. FOR FURTHER... of Homeland Security FR Federal Register NPRM Notice of Proposed Rulemaking A. Regulatory History and... Anchorage 9 (Mantua Creek Anchorage) due to dredging operations. The Dredge Florida will be working...

  5. Microwave DENSITY—INDEPENDENT Permittivity Functions as Spring Oats Kernels' Moisture Calibrators: a New Approach

    Science.gov (United States)

    Das, Priyanka; Ahmad, Zeeshan; Singh, P. N.; Prasad, Ashutosh

    2011-11-01

    The present work makes use of experimental data for real part of microwave complex permittivity of spring oats (Avena sativa L.) at 2.45 GHz and 24 °C as a function of moisture content, as extracted from the literature. These permittivity data were individually converted to those for solid materials using seven independent mixture equations for effective permittivity of random media. Moisture dependent quadratic models for complex permittivity of spring oats (Avena sativa L.), as developed by the present group, were used to evaluate the dielectric loss factor of spring oats kernels. Using these data, seven density—independent permittivity functions were evaluated and plotted as a function of moisture content of the samples. Second and third order polynomial regression equations were used for curve fittings with these data and their performances are reported. Coefficients of determination (r2) approaching unity (˜ 0.95-0.9999) and very small Standard Deviation (SD) ˜0.001-8.87 show good acceptability for these models. The regularity in the nature of these variations revealed the usefulness of the density—independent permittivity functions as indicators/calibrators of moisture content of spring oats kernels. Keeping in view the fact that moisture content of grains and seeds is an important factor determining quality and affecting the storage, transportation, and milling of grains and seeds, the work has the potentiality of its practical applications.

  6. Independent effects of both right and left ventricular function on plasma brain natriuretic peptide

    DEFF Research Database (Denmark)

    Vogelsang, Thomas Wiis; Jensen, Ruben J; Monrad, Astrid L;

    2007-01-01

    BACKGROUND: Brain natriuretic peptide (BNP) is increased in heart failure; however, the relative contribution of the right and left ventricles is largely unknown. AIM: To investigate if right ventricular function has an independent influence on plasma BNP concentration. METHODS: Right (RVEF), left...... ventricular ejection fraction (LVEF), and left ventricular end-diastolic volume index (LVEDVI) were determined in 105 consecutive patients by first-pass radionuclide ventriculography (FP-RNV) and multiple ECG-gated equilibrium radionuclide ventriculography (ERNV), respectively. BNP was analyzed by immunoassay......, which is a strong prognostic marker in heart failure, independently depends on both left and right ventricular systolic function. This might, at least in part, explain why BNP holds stronger prognostic value than LVEF alone....

  7. The benefit of differential moment concept in managing posterior anchorage and avoiding bite deepening

    Directory of Open Access Journals (Sweden)

    Harryanto Wijaya

    2012-09-01

    Full Text Available Background: Anchorage is one of the major concerns in orthodontic space closure. Various methods have been proposed to enhance posterior anchorage in space closure such as headgear, Nance holding appliance, and micro implant as temporary anchorage devices. However, several issues such as patient's compliance, appliance effectiveness, and cost of the device become many clinicians concern. The differential moment concept in segmented arch is a technique that requires no patient compliance but can effectively manage posterior anchorage and avoid bite deepening by careful application of forces and moments. Purpose: The purpose of this case report is to show the use of differential moment concept in segmented arch technique to manage posterior anchorage and to avoid bite deepening. Case: A 21 years old female patient with protrusive teeth as her chief complaint was treated using fixed orthodontic appliance. Case management: The treatment included four first bicuspid extraction and space closure utilizing differential moment concept in segmented arch. Conclusion: It can be concluded that application of differential moment concept in segmented arch technique is a non invasive, compliance independent, effective, and cost efficient method to manage posterior anchorage and to avoid bite deepening.latar belakang: Penjangkaran merupakan salah satu aspek yang sering kali menjadi masalah dalam penutupan ruang pada perawatan ortodonti. Berbagai metode disarankan untuk memperkuat penjangkaran posterior dalam penutupan ruang seperti headgear, piranti penahan Nance, dan implan mikro sebagai alat penjangkar sementara. Namun demikian, beberapa hal seperti kerjasama pasien, efektivitas piranti, dan biaya dari alat-alat tersebut sering menjadi perhatian/pertimbangan bagi klinisi. Konsep momen diferensial pada segmented arch adalah suatu cara yang efektif untuk memperkuat penjangkaran dan menghindari pendalaman gigitan tanpa memerlukan kerjasama pasien. tujuan

  8. Selective Functionalization of Independently Addressed Microelectrodes by Electrochemical Activation and Deactivation of a Coupling Catalyst

    OpenAIRE

    Devaraj, Neal. K.; Dinolfo, Peter H.; Chidsey, Christopher E. D.; Collman, James P.

    2006-01-01

    We demonstrate selective functionalization of independently addressed microelectrodes by electrochemical activation and deactivation of a coupling catalyst. 1,2,3-Triazole formation between terminal acetylenes and organic azides is efficiently catalyzed by copper(I) complexes (a Sharpless “click” reaction) while the oxidized copper (II) complexes are inactive. By electrochemically activating or deactivating the catalyst by switching its redox state, we demonstrate control over triazole format...

  9. Knee muscle strength, balance and functional independence in persons with stroke

    OpenAIRE

    Fernandes, B.; Batista, F; Beckert, P.; Ivangelista, I; Ferreira, MJ; Prates, L; Sérgio, J

    2011-01-01

    Purpose: To investigate the relationship between knee muscle strength, balance and functional independence within the first month after stroke. Relevance: Cerebral vascular disease is one of the main causes of morbidity, disability and mortality in developed countries. Problems with movement control are frequent after stroke. Lower limb weakness and impaired balance are common problems that are related with the risk of falls and are likely to interfere with the ability to perform dai...

  10. Directors’ Independence, Internal Audit Function, Ownership Concentration and Earnings Quality in Malaysia

    OpenAIRE

    Ahmed Hussein Al-Rassas; Hasnah Kamardin

    2015-01-01

    Concentration of ownership in Malaysian public listed companies contributes to agency conflict betweenmajority and minority shareholders. An effective monitoring mechanism is critical to mitigate this conflict. Thestudy aims to examine the influence of board and audit committee independence, internal audit function andownership concentration on earnings quality proxied by discretionary accruals. The sample of the study 508companies listed on the Bursa Malaysia Main Market from 2009 to 2012. T...

  11. P15.16EVOLUTION OF THE FUNCTIONAL INDEPENDENCE IN GLIOBLASTOMA PATIENTS

    OpenAIRE

    Sacko, A; Hou, M.; Temgoua, M.; Ursu-Billot, R.; Marantidou, A.; Doridam, J.; Coman, I.; Belin, C; Levy-Piedbois, C.; Carpentier, A F

    2014-01-01

    INTRODUCTION: Functional independence in glioblastoma (GBM) patients is a key factor to maintain quality of life. Progression free survival (PFS) and overall survival (OS) have been largely described in the literature. However, the evolution over time of the performance status remains poorly known. OBJECTIVE: To study the time to deterioration of the Karnosky Perfomance Status (KPS) below 70% in an homogeneous population of GBM patients. METHODS: We analyzed all patients treated in our instit...

  12. The influence of demographic, environmental and physical factors on functional independence post stroke

    OpenAIRE

    M.V. Mamabolo; W. Mudzi; A.S. Stewart; N. P. Mbambo; S. Olorunju

    2008-01-01

    Purpose: The magnitude of disability observed in strokesurvivors is believed to be dependent in part, on the severity of neurological deficits incurred. A s important but less well understood, is thecontribution of demographic, physical and environmental factors. The objective of this study was to establish what demographic, environmentaland physical factors influence functional independence post stroke. Method: Convenience sampling was used in the selection of subjects from four stroke outpa...

  13. Functional independence in patients with ischemic stroke in the chronic period

    OpenAIRE

    Vasileva, Dance; Lubenova, Daniela

    2013-01-01

    Objective: To investigate the influence of the specialized physical therapy on functional independence and to compare the impact of usual physical therapy in patients with ischemic stroke in the chronic period. Аn indisputable fact is that exercises have a positive contribution to the human body. It is well known that daily physical activities can improve the performance of many systems and organs by increasing its resistance and vital body tone, and also, can significantly improve the abi...

  14. Structural and functional characterization of a novel type of ligand-independent RXR-USP receptor

    OpenAIRE

    Iwema, Thomas; Billas, Isabelle ML; Beck, Yannick; Bonneton, François; Nierengarten, Hélène; Chaumot, Arnaud; Richards, Geoff; Laudet, Vincent; Moras, Dino

    2007-01-01

    Retinoid X receptor (RXR) and Ultraspiracle (USP) play a central role as ubiquitous heterodimerization partners of many nuclear receptors. While it has long been accepted that a wide range of ligands can activate vertebrate/mollusc RXRs, the existence and necessity of specific endogenous ligands activating RXR-USP in vivo is still matter of intense debate. Here we report the existence of a novel type of RXR-USP with a ligand-independent functional conformation. Our studies involved Tribolium ...

  15. "Real world" functioning in schizophrenia patients and healthy adults: assessing validity of the Multidimensional Scale of Independent Functioning.

    Science.gov (United States)

    Miles, Ashley A; Heinrichs, R Walter; Ammari, Narmeen

    2011-03-30

    As treatment efforts to enhance cognitive abilities in schizophrenia increase, so too does the need for a critical appraisal of instruments that measure functionality and adjustment to community living. The Multidimensional Scale of Independent Functioning (MSIF; Jaeger et al., 2003) is a promising instrument that assesses functionality in relation to different life settings, performance levels, responsibilities and environmental supports. However, its applicability to the schizophrenia population has been questioned because relevant data are scarce. This study provides descriptive and validity-related information by reporting MSIF scores in healthy community-dwelling adults (n=71) and in schizophrenia outpatients (n=156). Results show that healthy adults performed within defined "normal" ranges in most MSIF domains in comparison to schizophrenia patients who showed moderate to severe impairments. Moreover, the MSIF distinguished between the two groups with accuracy rates as high as 98% and effect sizes (standardized mean group difference) above 2.0 in almost all domains. Accordingly, the MSIF is a potentially valuable measure of community independence that can inform treatment initiatives and may be adaptable to the evaluation of functionality changes over time. The unique structure and content of information obtained by the MSIF makes it a candidate for inclusion in studies aimed at developing a new generation of instruments for the assessment of real world functioning in schizophrenia.

  16. Comparison of two functional independence scales with a participation measure in post-stroke rehabilitation.

    Science.gov (United States)

    Desrosiers, Johanne; Rochette, Annie; Noreau, Luc; Bravo, Gina; Hébert, Réjean; Boutin, Catherine

    2003-01-01

    The objectives of the study were to compare the association and responsiveness of the functional autonomy measurement system (SMAF) and functional independence measure (FIM) as outcome measures addressing functional independence in stroke patients involved in an intensive rehabilitation program and to compare their relationships with a social participation measure after rehabilitation period. One hundred and thirty-two people who had a stroke were evaluated with the SMAF and FIM during the rehabilitation period (T1: admission; T2: discharge; n=132) and twice after discharge (T3=2 weeks; n=118; T4=6 months later; n=102). At T3 and T4, a participation measure, the assessment of life habits (LIFE-H), was added. The main findings are: (1) the total scores on the SMAF and FIM are strongly correlated together (r=0.93 to 0.95; presponsiveness of both functional independence scales is similar even though the SMAF total score is more responsive to change than the FIM total score (standardized response mean: 1.20 vs. 0.97; pscale; and finally (4) the social roles domain of the participation scale is less related to the SMAF and MIF than the daily activities domain; however, the SMAF score is more related to the social roles domain than the MIF. Our results support the need to use supplementary measures, such as participation measure, that cover not only physical function but also the other domains of participation, such as interpersonal relationships and leisure, that can be disrupted following a stroke. PMID:12888229

  17. HIC-5: A Mobile Molecular Scaffold Regulating the Anchorage Dependence of Cell Growth

    Directory of Open Access Journals (Sweden)

    Motoko Shibanuma

    2012-01-01

    Full Text Available HIC-5 is a multidomain LIM protein homologous to paxillin that serves as a molecular scaffold at focal adhesions and in the nucleus. It forms mobile molecular units with LIM-only proteins, PINCH, and CRP2 and translocates in and out of the nucleus via a nuclear export signal (NES. Of note, NES of HIC-5 is distinctive in its sensitivity to the cellular redox state. Recently, the mobile units of HIC-5 have been suggested to be involved in the regulation of the anchorage dependence of cell growth. On loss of adhesion, an increase in reactive oxygen species in the cells modifies NES and stops shuttling, which leads to cell-cycle control. More specifically, the system circumvents nuclear localization of cyclin D1 and transactivates p21Cip1 in detached cells, thereby avoiding anchorage-independent cell growth. Thus, the HIC-5-LIM only protein complex has emerged as a fail-safe system for regulating the anchorage dependence of cell growth.

  18. Anchorage Arrival Scheduling Under Off-Nominal Weather Conditions

    Science.gov (United States)

    Grabbe, Shon; Chan, William N.; Mukherjee, Avijit

    2012-01-01

    Weather can cause flight diversions, passenger delays, additional fuel consumption and schedule disruptions at any high volume airport. The impacts are particularly acute at the Ted Stevens Anchorage International Airport in Anchorage, Alaska due to its importance as a major international portal. To minimize the impacts due to weather, a multi-stage scheduling process is employed that is iteratively executed, as updated aircraft demand and/or airport capacity data become available. The strategic scheduling algorithm assigns speed adjustments for flights that originate outside of Anchorage Center to achieve the proper demand and capacity balance. Similarly, an internal departure-scheduling algorithm assigns ground holds for pre-departure flights that originate from within Anchorage Center. Tactical flight controls in the form of airborne holding are employed to reactively account for system uncertainties. Real-world scenarios that were derived from the January 16, 2012 Anchorage visibility observations and the January 12, 2012 Anchorage arrival schedule were used to test the initial implementation of the scheduling algorithm in fast-time simulation experiments. Although over 90% of the flights in the scenarios arrived at Anchorage without requiring any delay, pre-departure scheduling was the dominant form of control for Anchorage arrivals. Additionally, tactical scheduling was used extensively in conjunction with the pre-departure scheduling to reactively compensate for uncertainties in the arrival demand. For long-haul flights, the strategic scheduling algorithm performed best when the scheduling horizon was greater than 1,000 nmi. With these long scheduling horizons, it was possible to absorb between ten and 12 minutes of delay through speed control alone. Unfortunately, the use of tactical scheduling, which resulted in airborne holding, was found to increase as the strategic scheduling horizon increased because of the additional uncertainty in the arrival times

  19. Independent effects of early-life experience and trait aggression on cardiovascular function.

    Science.gov (United States)

    Rana, Samir; Pugh, Phyllis C; Katz, Erin; Stringfellow, Sara A; Lin, Chee Paul; Wyss, J Michael; Stauss, Harald M; White, C Roger; Clinton, Sarah M; Kerman, Ilan A

    2016-08-01

    Early-life experience (ELE) can significantly affect life-long health and disease, including cardiovascular function. Specific dimensions of emotionality also modify risk of disease, and aggressive traits along with social inhibition have been established as independent vulnerability factors for the progression of cardiovascular disease. Yet, the biological mechanisms mediating these associations remain poorly understood. The present study utilized the inherently stress-susceptible and socially inhibited Wistar-Kyoto rats to determine the potential influences of ELE and trait aggression (TA) on cardiovascular parameters throughout the lifespan. Pups were exposed to maternal separation (MS), consisting of daily 3-h separations of the entire litter from postnatal day (P)1 to P14. The rats were weaned at P21, and as adults were instrumented for chronic radiotelemetry recordings of blood pressure and heart rate (HR). Adult aggressive behavior was assessed using the resident-intruder test, which demonstrated that TA was independent of MS exposure. MS-exposed animals (irrespective of TA) had significantly lower resting HR accompanied by increases in HR variability. No effects of MS on resting blood pressure were detected. In contrast, TA correlated with increased resting mean, systolic, and diastolic arterial pressures but had no effect on HR. TA rats (relative to nonaggressive animals) also manifested increased wall-to-lumen ratio in the thoracic aorta, increased sensitivity to phenylephrine-induced vascular contractility, and increased norepinephrine content in the heart. Together these data suggest that ELE and TA are independent factors that impact baseline cardiovascular function.

  20. Independent effects of early-life experience and trait aggression on cardiovascular function.

    Science.gov (United States)

    Rana, Samir; Pugh, Phyllis C; Katz, Erin; Stringfellow, Sara A; Lin, Chee Paul; Wyss, J Michael; Stauss, Harald M; White, C Roger; Clinton, Sarah M; Kerman, Ilan A

    2016-08-01

    Early-life experience (ELE) can significantly affect life-long health and disease, including cardiovascular function. Specific dimensions of emotionality also modify risk of disease, and aggressive traits along with social inhibition have been established as independent vulnerability factors for the progression of cardiovascular disease. Yet, the biological mechanisms mediating these associations remain poorly understood. The present study utilized the inherently stress-susceptible and socially inhibited Wistar-Kyoto rats to determine the potential influences of ELE and trait aggression (TA) on cardiovascular parameters throughout the lifespan. Pups were exposed to maternal separation (MS), consisting of daily 3-h separations of the entire litter from postnatal day (P)1 to P14. The rats were weaned at P21, and as adults were instrumented for chronic radiotelemetry recordings of blood pressure and heart rate (HR). Adult aggressive behavior was assessed using the resident-intruder test, which demonstrated that TA was independent of MS exposure. MS-exposed animals (irrespective of TA) had significantly lower resting HR accompanied by increases in HR variability. No effects of MS on resting blood pressure were detected. In contrast, TA correlated with increased resting mean, systolic, and diastolic arterial pressures but had no effect on HR. TA rats (relative to nonaggressive animals) also manifested increased wall-to-lumen ratio in the thoracic aorta, increased sensitivity to phenylephrine-induced vascular contractility, and increased norepinephrine content in the heart. Together these data suggest that ELE and TA are independent factors that impact baseline cardiovascular function. PMID:27280432

  1. The independent contribution of executive functions to health related quality of life in older women

    Directory of Open Access Journals (Sweden)

    Marra Carlo A

    2010-04-01

    Full Text Available Abstract Background Cognition is a multidimensional construct and to our knowledge, no previous studies have examined the independent contribution of specific domains of cognition to health related quality of life. To determine whether executive functions are independently associated with health related quality of life assessed using Quality Adjusted Life Years (QALYs calculated from the EuroQol EQ-5D (EQ-5D in older women after adjusting for known covariates, including global cognition. Therefore, we conducted a secondary analysis of community-dwelling older women aged 65-75 years who participated in a 12-month randomized controlled trial of resistance training. We assessed global cognition using the Mini-Mental State Examination (MMSE and executive functions using the: 1 Stroop Test; 2 Trail Making Test (Part B and 3 Digits Verbal Span Backwards Test. We calculated QALYs from the EQ-5D administered at baseline, 6 months and 12 months. Results Our multivariate linear regression model demonstrated the specific executive processes of set shifting and working memory, as measured by Trail Making Test (Part B and Digits Verbal Span Backward Test (p Conclusions Our study highlights the specific executive processes of set shifting and working memory were independently associated with QALYs -- a measure of health related quality of life. Given that executive functions explain variability in QALYs, clinicians may need to consider assessing executive functions when measuring health related quality of life. Further, the EQ-5D may be used to track changes in health status over time and serve as a screening tool for clinicians. Trial Registration ClinicalTrials.gov Identifier: NCT00426881.

  2. Functional independence and health-related functional status following spinal cord injury: A prospective study of the association with physical capacity

    OpenAIRE

    Haisma, Janneke; Post, Marcel; van der Woude, Lucas; Stam, Henk; Bergen, Michael; Sluis, Tebbe; Berg-Emons, Rita; Bussmann, Hans

    2008-01-01

    textabstractObjective: To determine changes in functional independence following spinal cord injury and to evaluate the association between functional independence and physical capacity. Design: Multi-centre prospective cohort study. Subjects: Patients with spinal cord injury admitted for initial rehabilitation. Methods: The motor Functional Independence Measure (FIMmotor) was determined at the start of rehabilitation (n=176), 3 months later (n=124), at discharge (n=160) and one year after di...

  3. Evolution of selenophosphate synthetases: emergence and relocation of function through independent duplications and recurrent subfunctionalization.

    Science.gov (United States)

    Mariotti, Marco; Santesmasses, Didac; Capella-Gutierrez, Salvador; Mateo, Andrea; Arnan, Carme; Johnson, Rory; D'Aniello, Salvatore; Yim, Sun Hee; Gladyshev, Vadim N; Serras, Florenci; Corominas, Montserrat; Gabaldón, Toni; Guigó, Roderic

    2015-09-01

    Selenoproteins are proteins that incorporate selenocysteine (Sec), a nonstandard amino acid encoded by UGA, normally a stop codon. Sec synthesis requires the enzyme Selenophosphate synthetase (SPS or SelD), conserved in all prokaryotic and eukaryotic genomes encoding selenoproteins. Here, we study the evolutionary history of SPS genes, providing a map of selenoprotein function spanning the whole tree of life. SPS is itself a selenoprotein in many species, although functionally equivalent homologs that replace the Sec site with cysteine (Cys) are common. Many metazoans, however, possess SPS genes with substitutions other than Sec or Cys (collectively referred to as SPS1). Using complementation assays in fly mutants, we show that these genes share a common function, which appears to be distinct from the synthesis of selenophosphate carried out by the Sec- and Cys- SPS genes (termed SPS2), and unrelated to Sec synthesis. We show here that SPS1 genes originated through a number of independent gene duplications from an ancestral metazoan selenoprotein SPS2 gene that most likely already carried the SPS1 function. Thus, in SPS genes, parallel duplications and subsequent convergent subfunctionalization have resulted in the segregation to different loci of functions initially carried by a single gene. This evolutionary history constitutes a remarkable example of emergence and evolution of gene function, which we have been able to trace thanks to the singular features of SPS genes, wherein the amino acid at a single site determines unequivocally protein function and is intertwined to the evolutionary fate of the entire selenoproteome.

  4. Independence of hot and cold executive function deficits in high-functioning adults with autism spectrum disorder

    Directory of Open Access Journals (Sweden)

    David Laird Zimmerman

    2016-02-01

    Full Text Available Individuals with autistic spectrum disorder (ASD display diverse deficits in social, cognitive and behavioral functioning. To date, there has been mixed findings on the profile of executive function deficits for high-functioning adults (IQ >70 with ASD. A conceptual distinction is commonly made between cold and hot executive functions. Cold executive functions refer to mechanistic higher-order cognitive operations (e.g., working memory, whereas hot executive functions entail cognitive abilities supported by emotional awareness and social perception (e.g., social cognition. This study aimed to determine the independence of deficits in hot and cold executive functions for high-functioning adults with ASD. Forty-two adults with ASD (64% male, aged 18-66 years and 40 age and gender matched controls were administered The Awareness of Social Inference Test (TASIT; emotion recognition and social inference, Letter Number Sequencing (working memory and Hayling Sentence Completion Test (response initiation and suppression. Between-group analyses identified that the ASD group performed significantly worse than matched controls on all measures of cold and hot executive functions (d = .54-1.5. Hierarchical multiple regression analyses revealed that the ASD sample performed more poorly on emotion recognition and social inference tasks than matched controls after controlling for cold executive functions and employment status. The findings also indicated that the ability to recognise emotions and make social inferences was supported by working memory and response initiation and suppression processes. Overall, this study supports the distinction between hot and cold executive function impairments for adults with ASD. Moreover, it advances understanding of higher-order impairments underlying social interaction difficulties for this population which, in turn, may assist with diagnosis and inform intervention programs.

  5. Independence of Hot and Cold Executive Function Deficits in High-Functioning Adults with Autism Spectrum Disorder.

    Science.gov (United States)

    Zimmerman, David L; Ownsworth, Tamara; O'Donovan, Analise; Roberts, Jacqueline; Gullo, Matthew J

    2016-01-01

    Individuals with autistic spectrum disorder (ASD) display diverse deficits in social, cognitive and behavioral functioning. To date, there has been mixed findings on the profile of executive function deficits for high-functioning adults (IQ > 70) with ASD. A conceptual distinction is commonly made between "cold" and "hot" executive functions. Cold executive functions refer to mechanistic higher-order cognitive operations (e.g., working memory), whereas hot executive functions entail cognitive abilities supported by emotional awareness and social perception (e.g., social cognition). This study aimed to determine the independence of deficits in hot and cold executive functions for high-functioning adults with ASD. Forty-two adults with ASD (64% male, aged 18-66 years) and 40 age and gender matched controls were administered The Awareness of Social Inference Test (TASIT; emotion recognition and social inference), Letter Number Sequencing (working memory) and Hayling Sentence Completion Test (response initiation and suppression). Between-group analyses identified that the ASD group performed significantly worse than matched controls on all measures of cold and hot executive functions (d = 0.54 - 1.5). Hierarchical multiple regression analyses revealed that the ASD sample performed more poorly on emotion recognition and social inference tasks than matched controls after controlling for cold executive functions and employment status. The findings also indicated that the ability to recognize emotions and make social inferences was supported by working memory and response initiation and suppression processes. Overall, this study supports the distinction between hot and cold executive function impairments for adults with ASD. Moreover, it advances understanding of higher-order impairments underlying social interaction difficulties for this population which, in turn, may assist with diagnosis and inform intervention programs. PMID:26903836

  6. Model independent evolution of transverse momentum dependent distribution functions (TMDs) at NNLL

    Energy Technology Data Exchange (ETDEWEB)

    Echevarria, Miguel G.; Scimemi, Ignazio [Universidad Complutense de Madrid (UCM), Departamento de Fisica Teorica II, Madrid (Spain); Idilbi, Ahmad [European Centre for Theoretical Studies in Nuclear Physics and Related Areas, ECT, Villazzano, Trento (Italy); Schaefer, Andreas [Universitaet Regensburg, Instituet fuer Theoretische Physik, Regensburg (Germany)

    2013-12-15

    We discuss the evolution of the eight leading-twist transverse momentum dependent parton distribution functions, which turns out to be universal and spin independent. By using the highest order perturbatively calculable ingredients at our disposal, we perform the resummation of the large logarithms that appear in the evolution kernel of transverse momentum distributions up to next-to-next-to-leading logarithms (NNLL), thus obtaining an expression for the kernel with highly reduced model dependence. Our results can also be obtained using the standard CSS approach when a particular choice of the b {sup *} prescription is used. In this sense, and while restricted to the perturbative domain of applicability, we consider our results as a ''prediction'' of the correct value of b{sub max} which is very close to 1.5 GeV{sup -1}. We explore under which kinematical conditions the effects of the non-perturbative region are negligible, and hence the evolution of transverse momentum distributions can be applied in a model independent way. The application of the kernel is illustrated by considering the unpolarized transverse momentum dependent parton distribution function and the Sivers function. (orig.)

  7. Model independent evolution of transverse momentum dependent distribution functions (TMDs) at NNLL

    International Nuclear Information System (INIS)

    We discuss the evolution of the eight leading-twist transverse momentum dependent parton distribution functions, which turns out to be universal and spin independent. By using the highest order perturbatively calculable ingredients at our disposal, we perform the resummation of the large logarithms that appear in the evolution kernel of transverse momentum distributions up to next-to-next-to-leading logarithms (NNLL), thus obtaining an expression for the kernel with highly reduced model dependence. Our results can also be obtained using the standard CSS approach when a particular choice of the b * prescription is used. In this sense, and while restricted to the perturbative domain of applicability, we consider our results as a ''prediction'' of the correct value of bmax which is very close to 1.5 GeV-1. We explore under which kinematical conditions the effects of the non-perturbative region are negligible, and hence the evolution of transverse momentum distributions can be applied in a model independent way. The application of the kernel is illustrated by considering the unpolarized transverse momentum dependent parton distribution function and the Sivers function. (orig.)

  8. The stability and equivalence reliability of the functional independence measure for children (WeeFIM).

    Science.gov (United States)

    Ottenbacher, K J; Taylor, E T; Msall, M E; Braun, S; Lane, S J; Granger, C V; Lyons, N; Duffy, L C

    1996-10-01

    The reliability of the Functional Independence Measure for Children (WeeFIM) was examined in 37 non-disabled children and 30 children with disabilities, from 12 to 76 months of age. The WeeFIM is derived from the Functional Independence Measure (FIM) and includes 18 items involving six functional subscales. Stability was assessed by administering the WeeFIM instrument to each child's caregiver on two occasions separated by 7 to 14 days. Intraclass correlation co-efficients (ICCs) for individual items ranged from 0.90 to 0.99. The ICC for the six WeeFIM subscales ranged from 0.94 for social cognition to 0.99 for transfers and locomotion. The ICC value for total WeeFIM test-retest reliability was 0.98 for children with disabilities and 0.99 for children without disabilities. Equivalence reliability was examined by comparing ratings obtained when using personal assessment with ratings collected during a telephone interview. No statistically significant differences were found for individual items, subscale scores or total WeeFIM values. PMID:8870612

  9. Urban contaminants project: Fish and Hood Creeks, Anchorage, Alaska

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — Urbanization has decreased water quality and adversely impacted biological communities in the lakes and streams of Anchorage, Alaska (Hock, 1981; Brabets, 1987;...

  10. Minor tooth movements using microimplant anchorage: case reports.

    Science.gov (United States)

    Sohn, Dong-Seok; Lee, Jung-Kwang; An, Kyung-Mi

    2008-03-01

    For the treatment of extruded or tipped molars, various conventional techniques have been used. But those methods may lead to undesirable movement of the anchorage units and lengthen treatment time because of limited tooth-borne anchorage potential. Introduction of microimplants as orthodontic anchorage has expanded treatment possibilities because of their advantages. Some advantages are a less complex surgical procedure, decrease in cost, immediate loading, and their ability to be placed in any area of the alveolar bone. This article will illustrate clinical experiences in patients who were treated with the intrusion of overerupted molars, the up-righting of tilted molars, and other clinical applications for minor tooth movements. Anchorage control was achieved with the surgical insertion of titanium microimplants for immediate loading in the alveolar bone. When needed, minimal fixed appliances were used and orthodontic treatment was completed without any other complications.

  11. (Prorenin receptor triggers distinct angiotensin II-independent extracellular matrix remodeling and deterioration of cardiac function.

    Directory of Open Access Journals (Sweden)

    Anne-Mari Moilanen

    Full Text Available BACKGROUND: Activation of the renin-angiotensin-system (RAS plays a key pathophysiological role in heart failure in patients with hypertension and myocardial infarction. However, the function of (prorenin receptor ((PRR is not yet solved. We determined here the direct functional and structural effects of (PRR in the heart. METHODOLOGY/PRINCIPAL FINDINGS: (PRR was overexpressed by using adenovirus-mediated gene delivery in normal adult rat hearts up to 2 weeks. (PRR gene delivery into the anterior wall of the left ventricle decreased ejection fraction (P<0.01, fractional shortening (P<0.01, and intraventricular septum diastolic and systolic thickness, associated with approximately 2-fold increase in left ventricular (PRR protein levels at 2 weeks. To test whether the worsening of cardiac function and structure by (PRR gene overexpression was mediated by angiotensin II (Ang II, we infused an AT(1 receptor blocker losartan via osmotic minipumps. Remarkably, cardiac function deteriorated in losartan-treated (PRR overexpressing animals as well. Intramyocardial (PRR gene delivery also resulted in Ang II-independent activation of extracellular-signal-regulated kinase1/2 phosphorylation and myocardial fibrosis, and the expression of transforming growth factor-β1 and connective tissue growth factor genes. In contrast, activation of heat shock protein 27 phosphorylation and apoptotic cell death by (PRR gene delivery was Ang II-dependent. Finally, (PRR overexpression significantly increased direct protein-protein interaction between (PRR and promyelocytic zinc-finger protein. CONCLUSIONS/SIGNIFICANCE: These results indicate for the first time that (PRR triggers distinct Ang II-independent myocardial fibrosis and deterioration of cardiac function in normal adult heart and identify (PRR as a novel therapeutic target to optimize RAS blockade in failing hearts.

  12. 78 FR 44917 - Anchorage Regulations; Port of New York

    Science.gov (United States)

    2013-07-25

    ...The Coast Guard proposes to establish and modify anchorage grounds within the Port of New York. This action is necessary to facilitate safe navigation and provide safe and secure anchorages for vessels operating in the area. This proposed rule is intended to increase the safety of life and property of both the anchored vessels and those operating in the area as well as provide for the overall......

  13. 76 FR 20524 - Anchorage Regulations; Port of New York

    Science.gov (United States)

    2011-04-13

    ...The Coast Guard is revising Anchorage Ground No. 19 located east of the Weehawken-Edgewater Federal Channel on the Hudson River. The revision is necessary to facilitate safe navigation and provide safe and secure anchorages for vessels operating in the area. This action is intended to increase the safety of life and property of both the anchored vessels and those operating in the area as well......

  14. Transcription-independent function of Polycomb group protein PSC in cell cycle control.

    Science.gov (United States)

    Mohd-Sarip, Adone; Lagarou, Anna; Doyen, Cecile M; van der Knaap, Jan A; Aslan, Ülkü; Bezstarosti, Karel; Yassin, Yasmin; Brock, Hugh W; Demmers, Jeroen A A; Verrijzer, C Peter

    2012-05-11

    Polycomb group (PcG) proteins control development and cell proliferation through chromatin-mediated transcriptional repression. We describe a transcription-independent function for PcG protein Posterior sex combs (PSC) in regulating the destruction of cyclin B (CYC-B). A substantial portion of PSC was found outside canonical PcG complexes, instead associated with CYC-B and the anaphase-promoting complex (APC). Cell-based experiments and reconstituted reactions established that PSC and Lemming (LMG, also called APC11) associate and ubiquitylate CYC-B cooperatively, marking it for proteosomal degradation. Thus, PSC appears to mediate both developmental gene silencing and posttranslational control of mitosis. Direct regulation of cell cycle progression might be a crucial part of the PcG system's function in development and cancer.

  15. Phase estimation from noisy phase fringe patterns using linearly independent basis functions

    International Nuclear Information System (INIS)

    A novel technique is proposed for obtaining unwrapped phase estimation from a highly noisy exponential phase field. In this technique, the interference phase is represented as a linear combination of linearly independent and pre-defined basis functions along each row/column of the phase field at a time. Consequently, the problem of phase estimation is converted into the problem of the estimation of the weights of the basis functions. The extended Kalman filter formulation allows for the accurate estimation of these weights. The simulation results indicate that the formulation offers a strong noise robustness in the phase estimation. Experimental results obtained using digital holographic interferometry and digital speckle pattern interferometry set-ups are provided to demonstrate the practical applicability of the proposed method. (paper)

  16. Unpolarized structure functions and the parton distributions for nucleon in an independent quark model

    Indian Academy of Sciences (India)

    N Barik; R N Mishra

    2001-04-01

    Considering the nucleon as consisting entirely of its valence quarks confined independently in a scalar-vector harmonic potential; unpolarized structure functions 1(,2) and 2(x,2) are derived in the Bjorken limit under certain simplifying assumptions; from which valence quark distribution functions (,2) and (,2) are appropriately extracted satisfying the normalization constraints. QCD-evolution of these input distributions from a model scale of 2=0.07 GeV2 to a higher $Q^{2}$ scale of $Q^{2}_{0} = 15$ GeV2 yields (, $Q^{2}_{0}$) and (, $Q^{2}_{0}$) in good agreement with experimental data. The gluon and sea-quark distributions such as (,$Q^{2}_{0}$) and (, $Q^{2}_{0}$) are dynamically generated with a reasonable qualitative agreement with the available data; using the leading order renormalization group equations with appropriate valence-quark distributions as the input.

  17. Personality traits, education, physical exercise, and childhood neurological function as independent predictors of adult obesity.

    Directory of Open Access Journals (Sweden)

    Helen Cheng

    Full Text Available OBJECTIVE: To investigate whether personality traits, education, physical exercise, parental socio-economic conditions, and childhood neurological function are independently associated with obesity in 50 year old adults in a longitudinal birth cohort study. METHOD: The sample consisted of 5,921 participants born in Great Britain in 1958 and followed up at 7, 11, 33, 42, and 50 years with data on body mass index measured at 42 and 50 years. RESULTS: There was an increase of adult obesity from 14.2% at age 42 to 23.6% at 50 years. Cohort members who were reported by teachers on overall clumsiness as "certainly applied" at age 7 were more likely to become obese at age 50. In addition, educational qualifications, traits Conscientiousness and Extraversion, psychological distress, and physical exercise were all significantly associated with adult obesity. The associations remained to be significant after controlling for birth weight and gestation, maternal and paternal BMI, childhood BMI, childhood intelligence and behavioural adjustment, as well as diet. CONCLUSION: Neurological function in childhood, education, trait Conscientiousness, and exercise were all significantly and independently associated with adult obesity, each explained unique individual variability.

  18. The urea carboxylase and allophanate hydrolase activities of urea amidolyase are functionally independent.

    Science.gov (United States)

    Lin, Yi; Boese, Cody J; St Maurice, Martin

    2016-10-01

    Urea amidolyase (UAL) is a multifunctional biotin-dependent enzyme that contributes to both bacterial and fungal pathogenicity by catalyzing the ATP-dependent cleavage of urea into ammonia and CO2 . UAL is comprised of two enzymatic components: urea carboxylase (UC) and allophanate hydrolase (AH). These enzyme activities are encoded on separate but proximally related genes in prokaryotes while, in most fungi, they are encoded by a single gene that produces a fusion enzyme on a single polypeptide chain. It is unclear whether the UC and AH activities are connected through substrate channeling or other forms of direct communication. Here, we use multiple biochemical approaches to demonstrate that there is no substrate channeling or interdomain/intersubunit communication between UC and AH. Neither stable nor transient interactions can be detected between prokaryotic UC and AH and the catalytic efficiencies of UC and AH are independent of one another. Furthermore, an artificial fusion of UC and AH does not significantly alter the AH enzyme activity or catalytic efficiency. These results support the surprising functional independence of AH from UC in both the prokaryotic and fungal UAL enzymes and serve as an important reminder that the evolution of multifunctional enzymes through gene fusion events does not always correlate with enhanced catalytic function.

  19. Measurement of functional independence level and falls-risk in individuals with undiagnosed phenylketonuria.

    LENUS (Irish Health Repository)

    Mazur, Artur

    2009-01-01

    The aim of the study was to determine the level of functional independence in adult patients with previously undiagnosed or untreated phenylketonuria (PKU). The study was conducted among 400 intellectually impaired adult residents of Social Welfare Homes in South-Eastern Poland born prior to the introduction of neonatal PKU screening programs. PKU was screened by filter paper test using tandem mass spectrometry methods, and confirmed by gas chromatography-mass spectrometric analysis of PKU organic acids in urine. Degree of functional independence included the assessment of activities of daily living (Barthel Index) and measures of balance and gait (Tinetti scale). Eleven individuals with previously untreated PKU were identified whereby eight presented with moderate disability and three with mild disability. Six had a high risk of falls and five had a moderate risk of falls. This study indicates that there is considerable number of undiagnosed PKU patients within the Polish population who require assessment and management in order to reduce the impact of the neurological and neuropsychiatric problems associated with the condition. Appropriate therapy for those with undiagnosed PKU should, in particular, address the risk of falls.

  20. Reduced lung function is independently associated with increased risk of type 2 diabetes in Korean men

    Directory of Open Access Journals (Sweden)

    Kwon Chang-Hee

    2012-04-01

    Full Text Available Abstract Background Reduced lung function is associated with incident insulin resistance and diabetes. The aim of this study was to assess the relationship between lung function and incident type 2 diabetes in Korean men. Methods This study included 9,220 men (mean age: 41.4 years without type 2 diabetes at baseline who were followed for five years. Subjects were divided into four groups according to baseline forced vital capacity (FVC (% predicted and forced expiratory volume in one second (FEV1 (% predicted quartiles. The incidence of type 2 diabetes at follow-up was compared according to FVC and FEV1 quartiles. Results The overall incidence of type 2 diabetes was 2.2%. Reduced lung function was significantly associated with the incidence of type 2 diabetes after adjusting for age, BMI, education, smoking, exercise, alcohol, and HOMA-IR. Both FVC and FEV1 were negatively associated with type 2 diabetes (P 1 had a significantly higher odds ratio for type 2 diabetes compared with the highest quartile after adjusting for age and BMI (2.15 [95% CI 1.02-4.57] and 2.19 [95% CI 1.09-4.42]. Conclusions Reduced lung function is independently associated with the incidence of type 2 diabetes in Korean men.

  1. High CO2 levels impair alveolar epithelial function independently of pH.

    Directory of Open Access Journals (Sweden)

    Arturo Briva

    Full Text Available BACKGROUND: In patients with acute respiratory failure, gas exchange is impaired due to the accumulation of fluid in the lung airspaces. This life-threatening syndrome is treated with mechanical ventilation, which is adjusted to maintain gas exchange, but can be associated with the accumulation of carbon dioxide in the lung. Carbon dioxide (CO2 is a by-product of cellular energy utilization and its elimination is affected via alveolar epithelial cells. Signaling pathways sensitive to changes in CO2 levels were described in plants and neuronal mammalian cells. However, it has not been fully elucidated whether non-neuronal cells sense and respond to CO2. The Na,K-ATPase consumes approximately 40% of the cellular metabolism to maintain cell homeostasis. Our study examines the effects of increased pCO2 on the epithelial Na,K-ATPase a major contributor to alveolar fluid reabsorption which is a marker of alveolar epithelial function. PRINCIPAL FINDINGS: We found that short-term increases in pCO2 impaired alveolar fluid reabsorption in rats. Also, we provide evidence that non-excitable, alveolar epithelial cells sense and respond to high levels of CO2, independently of extracellular and intracellular pH, by inhibiting Na,K-ATPase function, via activation of PKCzeta which phosphorylates the Na,K-ATPase, causing it to endocytose from the plasma membrane into intracellular pools. CONCLUSIONS: Our data suggest that alveolar epithelial cells, through which CO2 is eliminated in mammals, are highly sensitive to hypercapnia. Elevated CO2 levels impair alveolar epithelial function, independently of pH, which is relevant in patients with lung diseases and altered alveolar gas exchange.

  2. Seed germination strategies: an evolutionary trajectory independent of vegetative functional traits

    Directory of Open Access Journals (Sweden)

    Gemma eHoyle

    2015-10-01

    Full Text Available 1.Seed germination strategies vary dramatically among species but relatively little is known about how germination traits correlate with other elements of plant strategy systems. Understanding drivers of germination strategy is critical to our understanding of the evolutionary biology of plant reproduction.2.We present a novel assessment of seed germination strategies focussing on Australian alpine species as a case study. We describe the distribution of germination strategies and ask whether these are correlated with, or form an independent axis to, other plant functional traits. Our approach to describing germination strategy mimicked realistic temperatures that seeds experience in situ following dispersal. Strategies were subsequently assigned using an objective clustering approach. We hypothesised that two main strategies would emerge, involving dormant or non-dormant seeds, and that while these strategies would be correlated with seed traits (e.g. mass or endospermy they would be largely independent of vegetative traits when analysed in a phylogenetically structured manner. 3.Across all species, three germination strategies emerged. The majority of species postponed germination until after a period of cold, winter-like temperatures indicating physiological and/or morphological dormancy mechanisms. Other species exhibited immediate germination at temperatures representative of those at dispersal. Interestingly, seeds of an additional 13 species 'staggered' germination over time. Germination strategies were generally conserved within families. Across a broad range of ecological traits only seed mass and endospermy showed any correlation with germination strategy when phylogenetic relatedness was accounted for; vegetative traits showed no significant correlations with germination strategy. The results indicate that germination traits correlate with other aspects of seed ecology but form an independent axis relative to vegetative traits.

  3. Heat stress modifies human baroreflex function independently of heat-induced hypovolemia.

    Science.gov (United States)

    Kamiya, Atsunori; Michikami, Daisaku; Hayano, Junichiro; Sunagawa, Kenji

    2003-06-01

    Since human thermoregulatory heat loss responses, cutaneous vasodilation and sweating, cause hypovolemia, they should resultantly stimulate human baroreflexes. However, it is possible that the thermoregulatory system directly interacts with the baroreflex system through central neural connections independently of the heat-induced hypovolemia. We hypothesized that heat stress modifies the baroreflex control of sympathetic nerve activity independently of heat-induced hypovolemia in humans. We made whole-body heating with tube-lined suits perfused with warm water (46-47 degrees C) on 10 healthy male subjects. The heating increased skin and tympanic temperatures by 10.0 and 0.4 degrees C, respectively. It increased resting total muscle sympathetic nerve activity (MSNA, microneurography) by 94 +/- 9% and decreased central venous pressure (CVP, dependent arm technique) by 2.6 +/- 0.9 mmHg. The heating increased arterial baroreflex gain by 193%, assessed as a response of MSNA to a decrease in diastolic arterial pressure during Valsalva's maneuver, but it did not change threshold arterial pressure for MSNA activation. Although the heating did not change the cardiopulmonary baroreflex gain assessed as a response of MSNA to a change in estimated central venous pressure (CVP) during a 10 degrees head-down and -up tilt test, it upwardly shifted the stimulus-response baroreflex relationship. These changes in baroreflex functions during heating were not restored by an intravenous infusion of warmed isotonic saline (37 degrees C, 15 ml/kg) that restored the heat-induced reduction of CVP. Our results support our hypothesis that heat stress modifies the baroreflex control of MSNA independently of heat-induced hypovolemia in humans. Our results also suggest that the hyperthermal modification of baroreflex results from central neural interaction between thermoregulatory and baroreflex systems. PMID:14529582

  4. A numerical approach to model independently reconstruct $f(R)$ functions through cosmographic data

    CERN Document Server

    Pizza, Liberato

    2014-01-01

    The challenging issue of determining the correct $f(R)$ among several possibilities is here revised by means of numerical reconstructions of the modified Friedmann equations around the redshift interval $z\\in[0,1]$. Frequently, a severe degeneracy between $f(R)$ approaches occurs, since different paradigms correctly explain present time dynamics. To set the initial conditions on the $f(R)$ functions, we involve the use of the so called cosmography of the Universe, i.e. the technique of fixing constraints on the observable Universe by comparing expanded observables with current data. This powerful approach is essentially model independent and correspondingly we got a model independent reconstruction of $f(z)$ classes within the interval $z\\in[0,1]$. To allow the Hubble rate to evolve around $z\\leq1$, we considered three relevant frameworks of effective cosmological dynamics, i.e. the $\\Lambda$CDM model, the CPL parametrization and a polynomial approach to dark energy. Finally cumbersome algebra permits to pass...

  5. Acetylation of pregnane X receptor protein determines selective function independent of ligand activation

    International Nuclear Information System (INIS)

    Research highlights: → Pregnane X receptor (PXR), a major regulatory protein, is modified by acetylation. → PXR undergoes dynamic deacetylation upon ligand-mediated activation. → SIRT1 partially mediates PXR deacetylation. → PXR deacetylation per se induces lipogenesis mimicking ligand-mediated activation. -- Abstract: Pregnane X receptor (PXR), like other members of its class of nuclear receptors, undergoes post-translational modification [PTM] (e.g., phosphorylation). However, it is unknown if acetylation (a major and common form of protein PTM) is observed on PXR and, if it is, whether it is of functional consequence. PXR has recently emerged as an important regulatory protein with multiple ligand-dependent functions. In the present work we show that PXR is indeed acetylated in vivo. SIRT1 (Sirtuin 1), a NAD-dependent class III histone deacetylase and a member of the sirtuin family of proteins, partially mediates deacetylation of PXR. Most importantly, the acetylation status of PXR regulates its selective function independent of ligand activation.

  6. Spectral correlation functions of the sum of two independent complex Wishart matrices with unequal covariances

    Science.gov (United States)

    Akemann, Gernot; Checinski, Tomasz; Kieburg, Mario

    2016-08-01

    We compute the spectral statistics of the sum H of two independent complex Wishart matrices, each of which is correlated with a different covariance matrix. Random matrix theory enjoys many applications including sums and products of random matrices. Typically ensembles with correlations among the matrix elements are much more difficult to solve. Using a combination of supersymmetry, superbosonisation and bi-orthogonal functions we are able to determine all spectral k-point density correlation functions of H for arbitrary matrix size N. In the half-degenerate case, when one of the covariance matrices is proportional to the identity, the recent results by Kumar for the joint eigenvalue distribution of H serve as our starting point. In this case the ensemble has a bi-orthogonal structure and we explicitly determine its kernel, providing its exact solution for finite N. The kernel follows from computing the expectation value of a single characteristic polynomial. In the general non-degenerate case the generating function for the k-point resolvent is determined from a supersymmetric evaluation of the expectation value of k ratios of characteristic polynomials. Numerical simulations illustrate our findings for the spectral density at finite N and we also give indications how to do the asymptotic large-N analysis.

  7. Some Convex Functions Based Measures of Independence and Their Application to Strange Attractor Reconstruction

    Directory of Open Access Journals (Sweden)

    Kazuyuki Aihara

    2011-04-01

    Full Text Available The classical information-theoretic measures such as the entropy and the mutual information (MI are widely applicable to many areas in science and engineering. Csiszar generalized the entropy and the MI by using the convex functions. Recently, we proposed the grid occupancy (GO and the quasientropy (QE as measures of independence. The QE explicitly includes a convex function in its definition, while the expectation of GO is a subclass of QE. In this paper, we study the effect of different convex functions on GO, QE, and Csiszar’s generalized mutual information (GMI. A quality factor (QF is proposed to quantify the sharpness of their minima. Using the QF, it is shown that these measures can have sharper minima than the classical MI. Besides, a recursive algorithm for computing GMI, which is a generalization of Fraser and Swinney’s algorithm for computing MI, is proposed. Moreover, we apply GO, QE, and GMI to chaotic time series analysis. It is shown that these measures are good criteria for determining the optimum delay in strange attractor reconstruction.

  8. Attenuation of natural killer cell functions by capsaicin through a direct and TRPV1-independent mechanism.

    Science.gov (United States)

    Kim, Hun Sik; Kwon, Hyung-Joon; Kim, Gye Eun; Cho, Mi-Hyang; Yoon, Seung-Yong; Davies, Alexander J; Oh, Seog Bae; Lee, Heuiran; Cho, Young Keol; Joo, Chul Hyun; Kwon, Seog Woon; Kim, Sun Chang; Kim, Yoo Kyum

    2014-07-01

    The assessment of the biological activity of capsaicin, the compound responsible for the spicy flavor of chili pepper, produced controversial results, showing either carcinogenicity or cancer prevention. The innate immune system plays a pivotal role in cancer pathology and prevention; yet, the effect of capsaicin on natural killer (NK) cells, which function in cancer surveillance, is unclear. This study found that capsaicin inhibited NK cell-mediated cytotoxicity and cytokine production (interferon-γ and tumor necrosis factor-α). Capsaicin impaired the cytotoxicity of NK cells, thereby inhibiting lysis of standard target cells and gastric cancer cells by modulating calcium mobilization in NK cells. Capsaicin also induced apoptosis in gastric cancer cells, but that effect required higher concentrations and longer exposure times than those required to trigger NK cell dysfunction. Furthermore, capsaicin inhibited the cytotoxicity of isolated NK cells and of an NK cell line, suggesting a direct effect on NK cells. Antagonists of transient receptor potential vanilloid subfamily member 1 (TRPV1), a cognate capsaicin receptor, or deficiency in TRPV1 expression failed to prevent the defects induced by capsaicin in NK cells expressing functional TRPV1. Thus, the mechanism of action of capsaicin on NK cells is largely independent of TRPV1. Taken together, capsaicin may have chemotherapeutic potential but may impair NK cell function, which plays a central role in tumor surveillance. PMID:24743513

  9. Analysis of the Functional Independence Measure Value of Cervical Spine Injury Patients with Conservative Management

    Directory of Open Access Journals (Sweden)

    M. Zafrullah Arifin

    2012-06-01

    Full Text Available Analysis of the Functional Independence Measure Value of Cervical Spine Injury Patients with Conservative Management. Cervical spine injury is one of the most common spinal cord injuries in trauma patients. From 100,000 spinal cord injury cases reported in the United States of America (2008, sixty seven percent involve cervical spine injury. American Spinal Cord Injury Association (ASIA impairment score is used as an initial assessment but not enough attention prognostic outcome of these patients was paid to. The objective of this study is to analyze the value of functional independence measure (FIM cervical spine injury patients with conservative management and its correlation with age, sex, type of trauma, onset of trauma, cervical abnormalities, type of cervical spine lesion and ASIA impairment score. A prospective cohort study was performed to all patients with cervical spine injury treated inNeurosurgery Department of Dr. Hasan Sadikin Hospital Bandung that fullfiled the inclusion criteria. The subjects were classified based on age, sex, single/multiple trauma, acute /chronic, cervical abnormalities, complete/incomplete lesion and ASIA impairment score. The FIM examination was performed in Outpatient clinic of Neurosurgery. T-test and chi-square test was done to analyze the data. There were 17 cervical spine injury patients treated in Neurosurgery Department of Dr. Hasan Sadikin Hospital during April 2009–April 2010. The average FIM value of cervical spine injury in those patients is 4+ 1.63 by cohort prospective study. There were no correlation between FIM value with age, sex, type of trauma, onset of trauma and cervical abnormalities. Significant correlations were found between FIM value with type of cervical spine lesion and ASIA impairment score in cervical spine patients. Type of cervical spine lesion and ASIA impairment score have significant correlation with FIM value of patients in 6 months after cervical injury.

  10. Evaluation of functional recovery by motor functional independence measure test of elderly after hip fracture in Serbia.

    Science.gov (United States)

    Radosavljevic, Natasa; Nikolic, Dejan; Lazovic, Milica; Radosavljevic, Zoran; Jeremic, Aleksandar

    2014-01-01

    Introdução: o objectivo do estudo é a avaliação do nível de independência funcional através da aplicação da escala de Medida da Independência Funcional (Functional Independence Measure [FIM]) em doentes com idade superior a 65 anos, após fractura da anca.Material e Métodos: Foram estudados 203 doentes após fractura da anca, aplicando a escala MIF em 3 momentos: admissão do doente no hospital (Período 1), no momento da alta (Período 2) e 3 meses após a alta (Período 3); os doentes foram englobados em 3 grupos etários: Grupo65-74 , Grupo75-84 e Grupo+85 e em dois grupos, consoante o Índice de Gravidade (IG): grupo 0-1,99 (IG1) e grupo ≥ 2 (IG2).Resultados: No grupo de doentes com idêntico IG, observou-se um aumento dos valores da MIF no Período 2 e 3 em ambos osgéneros e nas primeiras duas classes etárias, ao passo que em doentes acima dos 85 anos, com IG mais elevado, observámos uma variação não significativa dos valores da MIF entre o momento da alta hospitalar e 3 meses após a alta.Discussão: A melhoria mais significativa da MIF foi obtida em doentes do sexo feminino no primeiro e terceiro grupos etários e com IG mais elevado.Conclusão: O género não constitui um factor preditivo significativo da recuperação da independência funcional medida através da aplicação da escala MIF em doentes com fractura da anca, embora a MIF no momento da admissão seja um bom indicador de recuperação funcional em doentes do sexo feminino em certos grupos etários (primeiro e terceiro grupos etários).

  11. Quadratic independence of coordinate functions of certain homogeneous spaces and action of compact quantum groups

    Indian Academy of Sciences (India)

    Debashish Goswami

    2015-02-01

    Let be one of the classical compact, simple, centre-less, connected Lie groups of rank with a maximal torus , the Lie algebra $\\mathcal{G}$ and let $\\{E_{i},F_{i},H_{i},i=1,\\ldots,n\\}$ be tha standard set of generators corresponding to a basis of the root system. Consider the adjoint-orbit space $M=\\{\\text{Ad}_{g}(H_{1}), g\\in G\\}$, identified with the homogeneous space / where $L=\\{g\\in G : \\text{Ad}_{g}(H_{1})=H_{1}\\}$. We prove that the coordinate functions $f_{i}(g):=_{i}(\\text{Ad}_{g}(H_{1}))$, $i=1,\\ldots,n$, where $\\{_{1},\\ldots,_{n}\\}$ is basis of $\\mathcal{G}'$ are `quadratically independent' in the sense that they do not satisfy any nontrivial homogeneous quadratic relations among them. Using this, it is proved that there is no genuine compact quantum group which can act faithfully on $C(M)$ such that the action leaves invariant the linear span of the above coordinate functions. As a corollary, it is also shown that any compact quantum group having a faithful action on the noncommutative manifold obtained by Rieffel deformation of satisfying a similar `linearity' condition must be a Rieffel-Wang type deformation of some compact group.

  12. The independent and interacting effects of hedonic hunger and executive function on binge eating.

    Science.gov (United States)

    Manasse, Stephanie M; Espel, Hallie M; Forman, Evan M; Ruocco, Anthony C; Juarascio, Adrienne S; Butryn, Meghan L; Zhang, Fengqing; Lowe, Michael R

    2015-06-01

    Poor executive function (EF; pre-frontal cognitive control processes governing goal-directed behavior) and elevated hedonic hunger (i.e., preoccupation with palatable foods in the absence of physiological hunger) are theoretical risk and maintenance factors for binge eating (BE) distinct from general obesity. Recent theoretical models posit that dysregulated behavior such as BE may result from a combination of elevated appetitive drive (e.g., hedonic hunger) and decreased EF (e.g., inhibitory control and delayed discounting). The present study sought to test this model in distinguishing BE from general obesity by examining the independent and interactive associations of EF and hedonic hunger with BE group status (i.e., odds of categorization in BE group versus non-BE group). Treatment-seeking overweight and obese women with BE (n = 31) and without BE (OW group; n = 43) were assessed on measures of hedonic hunger and EF (inhibitory control and delay discounting). Elevated hedonic hunger increased the likelihood of categorization in the BE group, regardless of EF. When hedonic hunger was low, poor EF increased the likelihood of categorization in the BE group. Results indicate that the interplay of increased appetitive drives and decreased cognitive function may distinguish BE from overweight/obesity. Future longitudinal investigations of the combinatory effect of hedonic hunger and EF in increasing risk for developing BE are warranted, and may inform future treatment development to target these factors.

  13. A Kinase-Independent Function of CDK6 Links the Cell Cycle to Tumor Angiogenesis

    Science.gov (United States)

    Kollmann, Karoline; Heller, Gerwin; Schneckenleithner, Christine; Warsch, Wolfgang; Scheicher, Ruth; Ott, Rene G.; Schäfer, Markus; Fajmann, Sabine; Schlederer, Michaela; Schiefer, Ana-Iris; Reichart, Ursula; Mayerhofer, Matthias; Hoeller, Christoph; Zöchbauer-Müller, Sabine; Kerjaschki, Dontscho; Bock, Christoph; Kenner, Lukas; Hoefler, Gerald; Freissmuth, Michael; Green, Anthony R.; Moriggl, Richard; Busslinger, Meinrad; Malumbres, Marcos; Sexl, Veronika

    2013-01-01

    Summary In contrast to its close homolog CDK4, the cell cycle kinase CDK6 is expressed at high levels in lymphoid malignancies. In a model for p185BCR-ABL+ B-acute lymphoid leukemia, we show that CDK6 is part of a transcription complex that induces the expression of the tumor suppressor p16INK4a and the pro-angiogenic factor VEGF-A. This function is independent of CDK6’s kinase activity. High CDK6 expression thus suppresses proliferation by upregulating p16INK4a, providing an internal safeguard. However, in the absence of p16INK4a, CDK6 can exert its full tumor-promoting function by enhancing proliferation and stimulating angiogenesis. The finding that CDK6 connects cell-cycle progression to angiogenesis confirms CDK6’s central role in hematopoietic malignancies and could underlie the selection pressure to upregulate CDK6 and silence p16INK4a. PMID:23948297

  14. A coding-independent function of an alternative Ube3a transcript during neuronal development.

    Science.gov (United States)

    Valluy, Jeremy; Bicker, Silvia; Aksoy-Aksel, Ayla; Lackinger, Martin; Sumer, Simon; Fiore, Roberto; Wüst, Tatjana; Seffer, Dominik; Metge, Franziska; Dieterich, Christoph; Wöhr, Markus; Schwarting, Rainer; Schratt, Gerhard

    2015-05-01

    The E3 ubiquitin ligase Ube3a is an important regulator of activity-dependent synapse development and plasticity. Ube3a mutations cause Angelman syndrome and have been associated with autism spectrum disorders (ASD). However, the biological significance of alternative Ube3a transcripts generated in mammalian neurons remains unknown. We report here that Ube3a1 RNA, a transcript that encodes a truncated Ube3a protein lacking catalytic activity, prevents exuberant dendrite growth and promotes spine maturation in rat hippocampal neurons. Surprisingly, Ube3a1 RNA function was independent of its coding sequence but instead required a unique 3' untranslated region and an intact microRNA pathway. Ube3a1 RNA knockdown increased activity of the plasticity-regulating miR-134, suggesting that Ube3a1 RNA acts as a dendritic competing endogenous RNA. Accordingly, the dendrite-growth-promoting effect of Ube3a1 RNA knockdown in vivo is abolished in mice lacking miR-134. Taken together, our results define a noncoding function of an alternative Ube3a transcript in dendritic protein synthesis, with potential implications for Angelman syndrome and ASD. PMID:25867122

  15. Functional and molecular characterization of mouse Gata2-independent hematopoietic progenitors.

    Science.gov (United States)

    Kaimakis, Polynikis; de Pater, Emma; Eich, Christina; Solaimani Kartalaei, Parham; Kauts, Mari-Liis; Vink, Chris S; van der Linden, Reinier; Jaegle, Martine; Yokomizo, Tomomasa; Meijer, Dies; Dzierzak, Elaine

    2016-03-17

    The Gata2 transcription factor is a pivotal regulator of hematopoietic cell development and maintenance, highlighted by the fact that Gata2 haploinsufficiency has been identified as the cause of some familial cases of acute myelogenous leukemia/myelodysplastic syndrome and in MonoMac syndrome. Genetic deletion in mice has shown that Gata2 is pivotal to the embryonic generation of hematopoietic stem cells (HSCs) and hematopoietic progenitor cells (HPCs). It functions in the embryo during endothelial cell to hematopoietic cell transition to affect hematopoietic cluster, HPC, and HSC formation. Gata2 conditional deletion and overexpression studies show the importance of Gata2 levels in hematopoiesis, during all developmental stages. Although previous studies of cell populations phenotypically enriched in HPCs and HSCs show expression of Gata2, there has been no direct study of Gata2 expressing cells during normal hematopoiesis. In this study, we generate a Gata2Venus reporter mouse model with unperturbed Gata2 expression to examine the hematopoietic function and transcriptome of Gata2 expressing and nonexpressing cells. We show that all the HSCs are Gata2 expressing. However, not all HPCs in the aorta, vitelline and umbilical arteries, and fetal liver require or express Gata2. These Gata2-independent HPCs exhibit a different functional output and genetic program, including Ras and cyclic AMP response element-binding protein pathways and other Gata factors, compared with Gata2-dependent HPCs. Our results, indicating that Gata2 is of major importance in programming toward HSC fate but not in all cells with HPC fate, have implications for current reprogramming strategies. PMID:26834239

  16. Clinical applications of orthodontic microimplant anchorage in craniofacial patients.

    Science.gov (United States)

    Vachiramon, Amornpong; Urata, Mark; Kyung, Hee Moon; Yamashita, Dennis-Duke; Yen, Stephen L-K

    2009-03-01

    Microimplant anchors, also known as temporary anchorage devices, mini- and micro-screws, have been used to enhance orthodontic anchorage for difficult tooth movements. Here, the authors describe how microimplants can be used to help treat craniofacial patients by supporting distraction osteogenesis procedures, maxillary protraction procedures, cleft segment expansion and stabilization, and tooth movement into narrow alveolar cleft sites. While most craniofacial patients are treated without microimplants, it would be worthwhile to identify which cases could benefit from microimplant anchorage. As an adjunct to orthodontic treatment, the microimplant offers a potential method for solving troublesome orthodontic and surgical problems such as guiding distraction procedures with orthodontics when primary teeth are exfoliating, addressing residual maxillary cants after vertical distraction osteogenesis of a ramus, stabilizing an edentulous premaxilla, and moving teeth into atrophic alveolar ridges. These cases are presented to open a dialogue on their possible uses in craniofacial patients.

  17. On the association between adolescent autonomy and psychosocial functioning: examining decisional independence from a self-determination theory perspective.

    Science.gov (United States)

    Van Petegem, Stijn; Beyers, Wim; Vansteenkiste, Maarten; Soenens, Bart

    2012-01-01

    In the present study, we focus on the concept of adolescent autonomy and its relation with psychosocial functioning. Specifically, we aim to differentiate between 2 prevailing conceptualizations of autonomy, that is, (a) autonomy defined as independence versus dependence and (b) autonomy defined as self-endorsed versus controlled functioning. A 2nd goal is to examine the relative contribution of each autonomy operationalization in the prediction of adolescents' adjustment (i.e., well-being, problem behavior, and intimacy). Data were gathered in a sample of 707 Belgian adolescents. Using a newly developed questionnaire, we assessed both the degree of independent decision making per se and the self-endorsed versus controlled motives underlying both independent and dependent decision making. The degree of independent decision making could clearly be differentiated from the underlying motives for doing so. Moreover, independent decision making as such showed unique associations with more problem behavior. Further, as expected, self-endorsed motives for both independent and dependent decision making generally related to an adaptive pattern of psychosocial functioning, and controlled motives were associated with maladjustment. The discussion focuses on the difference between the 2 perspectives on autonomy and on the different meaning of the motives underlying independent, relative to dependent, decision making.

  18. P15.16EVOLUTION OF THE FUNCTIONAL INDEPENDENCE IN GLIOBLASTOMA PATIENTS

    Science.gov (United States)

    Sacko, A.; Hou, M.; Temgoua, M.; Ursu-Billot, R.; Marantidou, A.; Doridam, J.; Coman, I.; Belin, C.; Levy-Piedbois, C.; Carpentier, A.F.

    2014-01-01

    INTRODUCTION: Functional independence in glioblastoma (GBM) patients is a key factor to maintain quality of life. Progression free survival (PFS) and overall survival (OS) have been largely described in the literature. However, the evolution over time of the performance status remains poorly known. OBJECTIVE: To study the time to deterioration of the Karnosky Perfomance Status (KPS) below 70% in an homogeneous population of GBM patients. METHODS: We analyzed all patients treated in our institution between 2008 and 2012. Patients were included if they met the following criteria: age >18 years, histologically confirmed GBM, supratentorial location, post-surgical KPS ≥70%, initial treatment with concomitant radiotherapy (RT) and Temozolomide (no Bevacizumab). Age, tumor location, surgical procedure, steroid dose at RT onset, KPS at 1 month after RT, then every 2 months until death, were collected in all patients. RESULTS: Within the 63 patients studied, median age was 61 years and 75 % underwent surgical resection at time of diagnosis. Forty-eight patients received one or several lines of chemotherapy at recurrence, and 34 patients (54%) were treated with Bevacizumab at one point during their evolution. The median PFS was 8.9 months and the median OS was 17.4 months. Interestingly, the median survival with KPS ≥70 (OS-70%) was 12 months, thus exceeding the median PFS by more than 3 months. OS-70% exceeded PFS by 6 months in 27% of the patients. Deterioration of KPS <70% occurred before progression in only 39% of the patients. Only surgical resection and low dose steroids (<30 mg equivalent prednisone) at RT onset, but not age ( 60 years), initial KPS, tumor location (frontal vs others), or seizures, were statistically associated with increased OS-70% (14,8 vs 4,5 months, p = 0.017; 16,2 vs 7,2 months, p = 0.001, respectively). DISCUSSION: In our series, the first recurrence after the initial treatment with RT and Temozolomide was not associated with loss of

  19. Application of an excited state LDA exchange energy functional for the calculation of transition energy of atoms within time-independent density functional theory

    Energy Technology Data Exchange (ETDEWEB)

    Shamim, Md; Harbola, Manoj K, E-mail: sami@iitk.ac.i, E-mail: mkh@iitk.ac.i [Department of Physics, Indian Institute of Technology, Kanpur 208 016 (India)

    2010-11-14

    Transition energies of a new class of excited states (two-gap systems) of various atoms are calculated in time-independent density functional formalism by using a recently proposed local density approximation exchange energy functional for excited states. It is shown that the excitation energies calculated with this functional compare well with those calculated with exact exchange theories.

  20. Regularization independent analysis of the origin of two loop contributions to N=1 Super Yang-Mills beta function

    Energy Technology Data Exchange (ETDEWEB)

    Fargnoli, H.G.; Sampaio, Marcos; Nemes, M.C. [Federal University of Minas Gerais, ICEx, Physics Department, P.O. Box 702, Belo Horizonte, MG (Brazil); Hiller, B. [Coimbra University, Faculty of Science and Technology, Physics Department, Center of Computational Physics, Coimbra (Portugal); Baeta Scarpelli, A.P. [Setor Tecnico-Cientifico, Departamento de Policia Federal, Lapa, Sao Paulo (Brazil)

    2011-05-15

    We present both an ultraviolet and an infrared regularization independent analysis in a symmetry preserving framework for the N=1 Super Yang-Mills beta function to two loop order. We show explicitly that off-shell infrared divergences as well as the overall two loop ultraviolet divergence cancel out, whilst the beta function receives contributions of infrared modes. (orig.)

  1. 76 FR 21633 - Disestablishing Special Anchorage Area 2; Ashley River, Charleston, SC

    Science.gov (United States)

    2011-04-18

    ... the developer has no riparian rights to the land beneath Ashley River Anchorage 2, and that the... the proposal by The City Marina should have any impact on disestablishing Ashley River Anchorage 2. While relocation of the channel could impact the location of part of Ashley River Anchorage 1, it...

  2. Retraining of interjoint arm coordination after stroke using robot-assisted time-independent functional training

    Directory of Open Access Journals (Sweden)

    Elizabeth B. Brokaw, MS

    2011-05-01

    Full Text Available We have developed a haptic-based approach for retraining of interjoint coordination following stroke called time-independent functional training (TIFT and implemented this mode in the ARMin III robotic exoskeleton. The ARMin III robot was developed by Drs. Robert Riener and Tobias Nef at the Swiss Federal Institute of Technology Zurich (Eidgenossische Technische Hochschule Zurich, or ETH Zurich, in Zurich, Switzerland. In the TIFT mode, the robot maintains arm movements within the proper kinematic trajectory via haptic walls at each joint. These arm movements focus training of interjoint coordination with highly intuitive real-time feedback of perform-ance; arm movements advance within the trajectory only if their movement coordination is correct. In initial testing, 37 nondisabled subjects received a single session of learning of a complex pattern. Subjects were randomized to TIFT or visual demonstration or moved along with the robot as it moved though the pattern (time-dependent [TD] training. We examined visual demonstration to separate the effects of action observation on motor learning from the effects of the two haptic guidance methods. During these training trials, TIFT subjects reduced error and interaction forces between the robot and arm, while TD subject performance did not change. All groups showed significant learning of the trajectory during unassisted recall trials, but we observed no difference in learning between groups, possibly because this learning task is dominated by vision. Further testing in stroke populations is warranted.

  3. Determination of arterial input function in dynamic susceptibility contrast MRI using group independent component analysis technique

    Science.gov (United States)

    Chen, Sharon; Liu, Ho-Ling; Yang, Yihong; Hsu, Yuan-Yu; Chuang, Keh-Shih

    2006-12-01

    Quantification of cerebral blood flow (CBF) with dynamic susceptibility contrast (DSC) magnetic resonance imaging (MRI) requires the determination of the arterial input function (AIF). The segmentation of surrounding tissue by manual selection is error-prone due to the partial volume artifacts. Independent component analysis (ICA) has the advantage in automatically decomposing the signals into interpretable components. Recently group ICA technique has been applied to fMRI study and showed reduced variance caused by motion artifact and noise. In this work, we investigated the feasibility and efficacy of the use of group ICA technique to extract the AIF. Both simulated and in vivo data were analyzed in this study. The simulation data of eight phantoms were generated using randomized lesion locations and time activity curves. The clinical data were obtained from spin-echo EPI MR scans performed in seven normal subjects. Group ICA technique was applied to analyze data through concatenating across seven subjects. The AIFs were calculated from the weighted average of the signals in the region selected by ICA. Preliminary results of this study showed that group ICA technique could not extract accurate AIF information from regions around the vessel. The mismatched location of vessels within the group reduced the benefits of group study.

  4. Effects of antidiabetes drugs on functional independence measure on a subacute rehabilitation ward for stroke patients.

    Science.gov (United States)

    Kose, E; Toyoshima, M; Tachi, T; Teramachi, H; Kawakubo, T; Hayashi, H

    2015-07-01

    It has been reported that the improvement of activities of daily living (ADL) by rehabilitation affects glycemic control. However, there are no reports about antidiabetes drugs as factors affecting the outcomes of rehabilitation. Therefore, we investigated the effects of antidiabetes drugs on functional independence measure (FIM) [total (T), motor (M), and cognition (C) items] in stroke patients with diabetes who were discharged from the subacute rehabilitation ward. We chose the frequently used antidiabetes drugs [sulfonylurea (SU), dipeptidyl peptidase-IV inhibitors (DPP-IVIs), and α-glycosidase inhibitors (α-GIs)] as the basis for categorizing the patients. We compared the patients' background features and laboratory data among the three groups. As a result, when SU was used in stroke patients with diabetes, it is difficult to obtain significant FIM-M gain, FIM-C gain, FIM-M efficiency, and FIM-C efficiency compared with of-GIs. As a reason for this, we hypothesize the possibility of the involvement of insulin resistance. Therefore, we consider that insulin resistance should be determined early and that it is important to reduce insulin resistance comprehensively by involving experts.

  5. A robust independent component analysis (ICA) model for functional magnetic resonance imaging (fMRI) data

    Science.gov (United States)

    Ao, Jingqi; Mitra, Sunanda; Liu, Zheng; Nutter, Brian

    2011-03-01

    The coupling of carefully designed experiments with proper analysis of functional magnetic resonance imaging (fMRI) data provides us with a powerful as well as noninvasive tool to help us understand cognitive processes associated with specific brain regions and hence could be used to detect abnormalities induced by a diseased state. The hypothesisdriven General Linear Model (GLM) and the data-driven Independent Component Analysis (ICA) model are the two most commonly used models for fMRI data analysis. A hybrid ICA-GLM model combines the two models to take advantages of benefits from both models to achieve more accurate mapping of the stimulus-induced activated brain regions. We propose a modified hybrid ICA-GLM model with probabilistic ICA that includes a noise model. In this modified hybrid model, a probabilistic principle component analysis (PPCA)-based component number estimation is used in the ICA stage to extract the intrinsic number of original time courses. In addition, frequency matching is introduced into the time course selection stage, along with temporal correlation, F-test based model fitting estimation, and time course combination, to produce a more accurate design matrix for GLM. A standard fMRI dataset is used to compare the results of applying GLM and the proposed hybrid ICA-GLM in generating activation maps.

  6. Pyrimidine Pathway-Dependent and -Independent Functions of the Toxoplasma gondii Mitochondrial Dihydroorotate Dehydrogenase.

    Science.gov (United States)

    Hortua Triana, Miryam Andrea; Cajiao Herrera, Daniela; Zimmermann, Barbara H; Fox, Barbara A; Bzik, David J

    2016-10-01

    Dihydroorotate dehydrogenase (DHODH) mediates the fourth step of de novo pyrimidine biosynthesis and is a proven drug target for inducing immunosuppression in therapy of human disease as well as a rapidly emerging drug target for treatment of malaria. In Toxoplasma gondii, disruption of the first, fifth, or sixth step of de novo pyrimidine biosynthesis induced uracil auxotrophy. However, previous attempts to generate uracil auxotrophy by genetically deleting the mitochondrion-associated DHODH of T. gondii (TgDHODH) failed. To further address the essentiality of TgDHODH, mutant gene alleles deficient in TgDHODH activity were designed to ablate the enzyme activity. Replacement of the endogenous DHODH gene with catalytically deficient DHODH gene alleles induced uracil auxotrophy. Catalytically deficient TgDHODH localized to the mitochondria, and parasites retained mitochondrial membrane potential. These results show that TgDHODH is essential for the synthesis of pyrimidines and suggest that TgDHODH is required for a second essential function independent of its role in pyrimidine biosynthesis. PMID:27481247

  7. Service provision and functional independence in depressed stroke patients and the effect of social work intervention on these.

    OpenAIRE

    Towle, D; Lincoln, N B; Mayfield, L M

    1989-01-01

    OBJECTIVE To compare the provision of health and social services between a social work intervention and non-intervention group. DESIGN Depressed stroke patients were randomly assigned to either an intervention or non-intervention group. Over a 4 month period both groups were visited three times by an independent assessor who rated them on service provision and functional independence. SETTING Patients' homes. PATIENTS 44 depressed stroke patients. INTERVENTION Both groups were given an inform...

  8. A Multidimensional Rasch Analysis of the Functional Independence Measure Based on the National Institute on Disability, Independent Living, and Rehabilitation Research Traumatic Brain Injury Model Systems National Database.

    Science.gov (United States)

    Pretz, Christopher R; Kean, Jacob; Heinemann, Allen W; Kozlowski, Allan J; Bode, Rita K; Gebhardt, Eveline

    2016-07-15

    A number of studies have evaluated the psychometric properties of the Functional Independence Measure (FIM™) using Rasch analysis, although none has done so using the National Institute on Disability, Independent Living, and Rehabilitation Research Traumatic Brain Injury Model Systems National Database, a longitudinal database that captures demographic and outcome information on persons with moderate to severe traumatic brain injury across the United States. In the current study, we examine the psychometric properties of the FIM as represented by persons within this database and demonstrate that the FIM comprises three subscales representing cognitive, self-care, and mobility domains. These subscales were analyzed simultaneously using a multivariate Rasch model in combination with a time dependent concurrent calibration scheme with the goal of creating a raw score-to-logit transformation that can be used to improve the accuracy of parametric statistical analyses. The bowel and bladder function items were removed because of misfit with the motor and cognitive items. Some motor items exhibited step disorder, which was addressed by collapsing Categories 1-3 for Toileting, Stairs, Locomotion, Tub/Shower Transfers; Categories 1 and 2 for Toilet and Bed Transfers; and Categories 2 and 3 for Grooming. The strong correlations (r = 0.82-0.96) among the three subscales suggest they should be modeled together. Coefficient alpha of 0.98 indicates high internal consistency. Keyform maps are provided to enhance clinical interpretation and application of study results.

  9. A Multidimensional Rasch Analysis of the Functional Independence Measure Based on the National Institute on Disability, Independent Living, and Rehabilitation Research Traumatic Brain Injury Model Systems National Database.

    Science.gov (United States)

    Pretz, Christopher R; Kean, Jacob; Heinemann, Allen W; Kozlowski, Allan J; Bode, Rita K; Gebhardt, Eveline

    2016-07-15

    A number of studies have evaluated the psychometric properties of the Functional Independence Measure (FIM™) using Rasch analysis, although none has done so using the National Institute on Disability, Independent Living, and Rehabilitation Research Traumatic Brain Injury Model Systems National Database, a longitudinal database that captures demographic and outcome information on persons with moderate to severe traumatic brain injury across the United States. In the current study, we examine the psychometric properties of the FIM as represented by persons within this database and demonstrate that the FIM comprises three subscales representing cognitive, self-care, and mobility domains. These subscales were analyzed simultaneously using a multivariate Rasch model in combination with a time dependent concurrent calibration scheme with the goal of creating a raw score-to-logit transformation that can be used to improve the accuracy of parametric statistical analyses. The bowel and bladder function items were removed because of misfit with the motor and cognitive items. Some motor items exhibited step disorder, which was addressed by collapsing Categories 1-3 for Toileting, Stairs, Locomotion, Tub/Shower Transfers; Categories 1 and 2 for Toilet and Bed Transfers; and Categories 2 and 3 for Grooming. The strong correlations (r = 0.82-0.96) among the three subscales suggest they should be modeled together. Coefficient alpha of 0.98 indicates high internal consistency. Keyform maps are provided to enhance clinical interpretation and application of study results. PMID:26559881

  10. Structure-function analysis of Methanobacterium thermoautotrophicum RNA ligase – engineering a thermostable ATP independent enzyme

    Directory of Open Access Journals (Sweden)

    Zhelkovsky Alexander M

    2012-07-01

    Mutational analysis of the heat stable RNA ligase from Methanobacterium thermoautotrophicum resulted in the creation of an ATP independent ligase. The K97A mutant is defective in the first two steps of ligation but retains full activity in ligation of either RNA or ssDNA to a pre-adenylated linker. The ability of the ligase to function at 65°C should reduce the constraints of RNA secondary structure in RNA ligation experiments.

  11. Corticotomy-assisted molar protraction with the aid of temporary anchorage device.

    Science.gov (United States)

    Uribe, Flavio; Janakiraman, Nandakumar; Fattal, Amine N; Schincaglia, Gian Pietro; Nanda, Ravindra

    2013-11-01

    This case report describes the interdisciplinary management of a 58-year-old woman who was missing lower first molars and supraerupted maxillary first molars. The treatment plan included intrusion of the upper first molars and corticotomy-assisted mandibular second molar protraction with the aid of temporary anchorage devices. Miniscrews were effective in intrusion of the maxillary first molars and protraction of the lower second molars. Although good functional outcome was achieved in 41 months, the corticotomy-assisted procedure did not significantly reduce the treatment time. PMID:23834274

  12. Independence Centrality as a Moderator of the Effects of Spousal Support on Patient Well-Being and Physical Functioning

    Science.gov (United States)

    Martire, Lynn M.; Stephens, Mary Ann Parris; Schulz, Richard

    2014-01-01

    Objective In this study, we examined whether the benefits of spousal assistance for patient well-being and physical functioning depend on the fit between amount of assistance provided and the personal importance of completing activities independently. Methods Individuals with osteoarthritis of the hip or knee were assessed for independence centrality, depressive symptoms, self-efficacy for managing pain, and physical functioning (N = 159 to 230). Spouses reported the amount of support provided with daily tasks. Results As predicted, moderation analyses indicated that spousal support was associated with greater self-efficacy for managing pain in patients with low independence centrality, but was not associated with self-efficacy in patients with high independence centrality. Also consistent with our hypotheses, spousal support was associated with greater depressive symptoms and slower walk time in patients with high independence centrality, but there were no effects of spouse support on these outcomes for patients with low independence centrality. Conclusions Our findings demonstrate the implications of miscarried spousal support for patient well-being and physical functioning, and they suggest a means of tailoring couple-oriented interventions for chronic illness. PMID:21534676

  13. Validación de la Escala de Independencia Funcional Validation of the Functional Independence Scale

    Directory of Open Access Journals (Sweden)

    Pablo Martínez-Martín

    2009-02-01

    Full Text Available Objetivo: Evaluar la calidad psicométrica de un instrumento diseñado para medir la independencia funcional (Escala de Independencia Funcional, EIF en varios dominios de actividades de la vida diaria y ser aplicado por entrevistadores entrenados no expertos en el ámbito sanitario. El estudio se realizó en población mayor no institucionalizada residente en la Comunidad de Madrid. Métodos: Estudio transversal de validación. Se aplicaron la EIF, el test de Pfeiffer, la subescala de depresión de la Hospital Anxiety and Depression Scale, un indicador de comorbilidad, el Índice de Barthel y el EQ-5D, a población residente en medio comunitario (n=500 y a pacientes ambulatorios en medio hospitalario (n=100 de edad ≥65 años. Se analizaron los siguientes atributos psicométricos de la EIF: aceptabilidad, asunciones escalares, consistencia interna, validez de constructo y precisión. Resultados: La escala resultó totalmente computable en el 94,3% de los sujetos, con efecto techo (60,65% y sin efecto suelo (0,22% en el medio comunitario. En el medio hospitalario no se evidenció efecto suelo ni techo. La escala mostró asunciones escalares satisfactorias y elevada consistencia interna (correlaciones ítem-total: 0,57-0,91; alfa de Cronbach: 0,94, así como una estructura multidimensional (tres factores; 74,3% de la varianza. Los índices de validez convergente, interna y para grupos conocidos, al igual que la precisión (error estándar de la medida: 2,49; intervalo de confianza del 95%: 4,88 resultaron satisfactorios. Conclusiones: En suma, la EIF es una escala de uso sencillo con atributos métricos apropiados, y su aplicación por parte de personal no sanitario resulta útil para muestras amplias de individuos mayores no institucionalizados.Objective: To assess the psychometric quality of an instrument designed to measure functional independence (Functional Independence Scale [FIS] in several activities of daily living domains and to be

  14. Shear and anchorage behaviour of fire exposed hollow core slabs

    NARCIS (Netherlands)

    Fellinger, J.

    2005-01-01

    The fire resistance of hollow core slabs is currently assessed considering flexural failure only. However, fire tests show that shear or anchorage failure can also govern the load bearing behaviour. This paper is based on the dissertation by the author1 and discusses existing and new fire tests. It

  15. 75 FR 3641 - Television Broadcasting Services; Anchorage, AK

    Science.gov (United States)

    2010-01-22

    ... From the Federal Register Online via the Government Publishing Office FEDERAL COMMUNICATIONS COMMISSION 47 CFR Part 73 Television Broadcasting Services; Anchorage, AK AGENCY: Federal Communications... Congressional review Act, see 5 U.S.C. 801(a)(1)(A). List of Subjects in 47 CFR Part 73 Television,...

  16. 75 FR 22323 - Anchorage Regulations; Port of New York

    Science.gov (United States)

    2010-04-28

    ... SECURITY Coast Guard 33 CFR Part 110 RIN 1625-AA01 Anchorage Regulations; Port of New York AGENCY: Coast...-mail Mr. Jeff Yunker, Coast Guard Sector New York, Waterways Management Division; telephone 718-354..., 2008 issue of the Federal Register (73 FR 3316). Public Meeting We do not now plan to hold a...

  17. External post-tensioning of cfrp tendons using integrated sleeve-wedge anchorage

    DEFF Research Database (Denmark)

    Schmidt, Jacob Wittrup; Bennitz, Anders; Goltermann, Per;

    2012-01-01

    Strengthening of structures using external post-tension CFRP systems have proven to be anefficient method as such system increases the structural capacity and reduces cracks and deflection. Sufficient anchorage is of significant importance since the anchorage provides the connection between...... anchorage was obtained using the novel anchorage and that its behaviour is stable and predicatble when short term static load is applied. Desired strengthening was also observed in external post-tensioning on reinforced concrete T-beams. The requirements and definitions on a stable anchorage of CFRP tendons...

  18. Influence of kinesitherapy on motor recovery and functional independence in patients with ischemic stroke in the chronic period

    OpenAIRE

    Vasileva, Dance; Lubenova, Daniela; Mihova, Marija; Grigorova-Petrova, Kristin; Dimitrova, Antoaneta

    2015-01-01

    The study aims to trace the influence of specialized kinesitherapeutic methodology (SKTM) on motor recovery and functional independence in patients with ischemic stroke in the chronic period (ISChP). Material and Methods The study was conducted with 56 patients with ISChP (32 men and 24 women, mean age 63.2 ± 8.8 years old, weight 77.9 ± 10.1 kg, height 169.2 ± 6.4 centimeters and duration of illness up to 1 year). Assessment of motor recovery and the level of functional independence...

  19. Characterizing tissue microstructure using an ultrasound system-independent spatial autocorrelation function

    Science.gov (United States)

    Dong, Fang

    1999-09-01

    The research described in this dissertation is related to characterization of tissue microstructure using a system- independent spatial autocorrelation function (SAF). The function was determined using a reference phantom method, which employed a well-defined ``point- scatterer'' reference phantom to account for instrumental factors. The SAF's were estimated for several tissue-mimicking (TM) phantoms and fresh dog livers. Both phantom tests and in vitro dog liver measurements showed that the reference phantom method is relatively simple and fairly accurate, providing the bandwidth of the measurement system is sufficient for the size of the scatterer being involved in the scattering process. Implementation of this method in clinical scanner requires that distortions from patient's body wall be properly accounted for. The SAF's were estimated for two phantoms with body-wall-like distortions. The experimental results demonstrated that body wall distortions have little effect if echo data are acquired from a large scattering volume. One interesting application of the SAF is to form a ``scatterer size image''. The scatterer size image may help providing diagnostic tools for those diseases in which the tissue microstructure is different from the normal. Another method, the BSC method, utilizes information contained in the frequency dependence of the backscatter coefficient to estimate the scatterer size. The SAF technique produced accurate scatterer size images of homogeneous TM phantoms and the BSC method was capable of generating accurate size images for heterogeneous phantoms. In the scatterer size image of dog kidneys, the contrast-to-noise-ratio (CNR) between renal cortex and medulla was improved dramatically compared to the gray- scale image. The effect of nonlinear propagation was investigated by using a custom-designed phantom with overlaying TM fat layer. The results showed that the correlation length decreased when the transmitting power increased. The

  20. Human primary adipocytes exhibit immune cell function: adipocytes prime inflammation independent of macrophages.

    Directory of Open Access Journals (Sweden)

    Kees Meijer

    Full Text Available BACKGROUND: Obesity promotes inflammation in adipose tissue (AT and this is implicated in pathophysiological complications such as insulin resistance, type 2 diabetes and cardiovascular disease. Although based on the classical hypothesis, necrotic AT adipocytes (ATA in obese state activate AT macrophages (ATM that then lead to a sustained chronic inflammation in AT, the link between human adipocytes and the source of inflammation in AT has not been in-depth and systematically studied. So we decided as a new hypothesis to investigate human primary adipocytes alone to see whether they are able to prime inflammation in AT. METHODS AND RESULTS: Using mRNA expression, human preadipocytes and adipocytes express the cytokines/chemokines and their receptors, MHC II molecule genes and 14 acute phase reactants including C-reactive protein. Using multiplex ELISA revealed the expression of 50 cytokine/chemokine proteins by human adipocytes. Upon lipopolysaccharide stimulation, most of these adipocyte-associated cytokines/chemokines and immune cell modulating receptors were up-regulated and a few down-regulated such as (ICAM-1, VCAM-1, MCP-1, IP-10, IL-6, IL-8, TNF-α and TNF-β highly up-regulated and IL-2, IL-7, IL-10, IL-13 and VEGF down-regulated. In migration assay, human adipocyte-derived chemokines attracted significantly more CD4+ T cells than controls and the number of migrated CD4+ cells was doubled after treating the adipocytes with LPS. Neutralizing MCP-1 effect produced by adipocytes reduced CD4+ migration by approximately 30%. CONCLUSION: Human adipocytes express many cytokines/chemokines that are biologically functional. They are able to induce inflammation and activate CD4+ cells independent of macrophages. This suggests that the primary event in the sequence leading to chronic inflammation in AT is metabolic dysfunction in adipocytes, followed by production of immunological mediators by these adipocytes, which is then exacerbated by

  1. Changes in executive functions and self-efficacy are independently associated with improved usual gait speed in older women

    Directory of Open Access Journals (Sweden)

    Hsu Chun

    2010-05-01

    Full Text Available Abstract Background Improved usual gait speed predicts substantial reduction in mortality. A better understanding of the modifiable factors that are independently associated with improved gait speed would ensure that intervention strategies are developed based on a valid theoretical framework. Thus, we examined the independent association of change in executive functions and change in falls-related self-efficacy with improved gait speed among community-dwelling senior women. Methods A secondary analysis of the 135 senior women aged 65 to 75 years old who completed a 12-month randomized controlled trial of resistance training. Usual gait speed was assessed using a 4-meter walk. Three executive processes were assessed by standard neuropsychological tests: 1 set shifting; 2 working memory; and 3 selective attention and response inhibition. A linear regression model was constructed to determine the independent association of change in executive functions and falls-related self-efficacy with change in gait speed. Results Improved selective attention and conflict resolution, and falls-related self-efficacy, were independently associated with improved gait speed after accounting for age, global cognition, baseline gait speed, and change in quadriceps strength. The total variance explained was 24%. Conclusions Interventions that target executive functions and falls-related self-efficacy, in addition to physical functions, to improve gait speed may be more efficacious than those that do not. Trial Registration ClinicalTrials.gov Identifier: NCT00426881

  2. Automatic denoising of functional MRI data: combining independent component analysis and hierarchical fusion of classifiers

    NARCIS (Netherlands)

    Salimi-Khorshidi, G.; Douaud, G.; Beckmann, C.F.; Glasser, M.F.; Griffanti, L.; Smith, S.M.

    2014-01-01

    Many sources of fluctuation contribute to the fMRI signal, and this makes identifying the effects that are truly related to the underlying neuronal activity difficult. Independent component analysis (ICA) - one of the most widely used techniques for the exploratory analysis of fMRI data - has shown

  3. Automatic denoising of functional MRI data: Combining independent component analysis and hierarchical fusion of classifiers

    NARCIS (Netherlands)

    Salimi-Khorshidi, Gholamreza; Douaud, Gwenaelle; Beckmann, Christian F.; Glasser, Matthew F.; Griffanti, Ludovica; Smith, Stephen M.

    2014-01-01

    Many sources of fluctuation contribute to the fMRI signal, and this makes identifying the effects that are truly related to the underlying neuronal activity difficult. Independent component analysis (ICA) – one of the most widely used techniques for the exploratory analysis of fMRI data – has shown

  4. Is impaired kidney function an independent predictor of the risk of myocardial infarction in HIV-infected individuals?

    Science.gov (United States)

    Lang, Sylvie; Mary-Krause, Murielle; Partisani, Marialuisa; Gilquin, Jacques; Simon, Anne; Cotte, Laurent; Boccara, Franck; Costagliola, Dominique

    2014-08-24

    We examined whether impaired kidney function is an independent risk factor for myocardial infarction in HIV-infected individuals without pre-existing coronary artery disease. The odds ratio for impaired kidney function fell from 1.22 (95% confidence interval 0.90-1.66) to 0.99 (95% confidence interval 0.69-1.41) after adjustment for cardiovascular risk factors and HIV-related parameters, with hypertension, high-density lipoprotein cholesterol, smoking and the CD4 T-cell nadir as most influential confounders. In this setting, no association was found between impaired kidney function and the risk of myocardial infarction.

  5. Requirements of transcription factor Smad-dependent and -independent TGF-β signaling to control discrete T-cell functions

    OpenAIRE

    Gu, Ai-di; Wang, Yunqi; Lin, Lin; Zhang, Song S.; Wan, Yisong Y.

    2012-01-01

    TGF-β modulates immune response by suppressing non-regulatory T (Treg) function and promoting Treg function. The question of whether TGF-β achieves distinct effects on non-Treg and Treg cells through discrete signaling pathways remains outstanding. In this study, we investigated the requirements of Smad-dependent and -independent TGF-β signaling for T-cell function. Smad2 and Smad3 double deficiency in T cells led to lethal inflammatory disorder in mice. Non-Treg cells were spontaneously acti...

  6. Relationship between physical functioning and physical activity in the lifestyle interventions and independence for elders pilot

    Science.gov (United States)

    OBJECTIVES: To determine whether participation in usual moderate-intensity or more-vigorous physical activity (MVPA) is associated with physical function performance and to identify sociodemographic, psychosocial, and disease-related covariates that may also compromise physical function performance....

  7. Molar distalization with the assistance of Temporary Anchorage Devices.

    Science.gov (United States)

    Palencar, Adrian J

    2015-01-01

    This article describes efficient techniques for distalization of maxillary and mandibular molars with the assistance of Temporary Anchorage Devices (TADs). There are numerous occasions where the distalization of molars is required in lieu of the odontectomy of bicuspids. In the past, extra-oral force has been used, (i.e. Cervical or Combination Head Gear, or intra-oral force, i.e. Posterior Sagittal Appliance, Modified Greenfield Appliance, Williams DMJ 20001, CD Distalizer, Magill Sagittal, Pendulum Appliance, etc.). All the intra-oral appliances have a common denominator the orthodontic clinician has to deal with, the undesirable expression of the Third Law of Newton. The utilization of TADs allows us to circumvent this shortcoming, establishing an absolute anchorage, and thus completely negate the expression of the Third Law of Newton. PMID:25881377

  8. Covariant and background independent functional RG flow for the effective average action

    CERN Document Server

    Safari, Mahmoud

    2016-01-01

    We extend our prescription for the construction of a covariant and background-independent effective action for scalar quantum field theories to the case where momentum modes below a certain scale are suppressed by the presence of an infrared regulator. The key step is an appropriate choice of the infrared cutoff for which the Ward identity, capturing the information from single-field dependence of the ultraviolet action, continues to be exactly solvable, and therefore, in addition to covariance, manifest background independence of the effective action is guaranteed at any scale. A practical consequence is that in this framework one can adopt truncations dependent on the single total field. Furthermore we discuss the necessary and sufficient conditions for the preservation of symmetries along the renormalization group flow.

  9. Economical preconditions of functioning of independent boards of directors in view of neoclassical economical theory

    OpenAIRE

    Andrey, Rudkov

    2011-01-01

    The article examines analytically economical preconditions and motivation of economical agents to use independent boards in corporate government of a firm. Behavior of economical agents (shareholders and management of a firm) is analyzed in view of neoclassical economical theory. In survey author analyzes behavior of shareholders and makes conclusion that for shareholder the most efficient way to minimize transactional costs while being invested in stocks of a company is to delegate some func...

  10. The growth factor independence-1 transcription factor: New functions and new insights☆

    OpenAIRE

    Kazanjian, Avedis; Gross, Eleanore A.; Grimes, H. Leighton

    2006-01-01

    The growth factor independence-1 (Gfi1) transcription factor is required for proper development of neuroendocrine cells, sensory neurons, and blood. Patients with mutations in Gfi1 exhibit severe congenital neutropenia (SCN) or non-immune chronic idiopathic neutropenia of adults. Gfi1 was initially described as an oncoprotein that mediates tumor progression in a mouse model of leukemia; however, recent data suggest that Gfi1 may act as either an oncogene or an anti-proliferative tumor suppres...

  11. Virtual planning for vertical control using temporary anchorage devices

    OpenAIRE

    Mauricio Accorsi

    2015-01-01

    The new and innovative technologies are unprecedentedly improving the level of proficiency in orthodontics in the recent history of this area of expertise. The proliferation of advances, such as self-ligating systems, temporary anchorage devices, shape-memory wires, robotically wire bending, intraoral scanners, cone-beam computed tomography, bring the virtual planning, and confection of dental devices through CAD/CAM systems to the real world. In order to get efficiency and efficacy in orthod...

  12. Tensile tests of basalt FRP rebars using chemical anchorages

    OpenAIRE

    Neagoe, Catalin Andrei; Gil Espert, Lluís

    2013-01-01

    In the current report are analyzed the experimental results of initial tensile tests of Basalt FRP (BFRP) rebars provided by VSL. The investigation was conducted in the laboratory of CER LITEM. Objectives of the study: to determine the tensile strengths of 6 mm and 8 mm basalt FRP rebars, and to determine what type of test setup and anchorage system is best suited for future tensile tests of BFRP bars.

  13. Tensile tests of basalt FRP rebars using mechanical anchorages

    OpenAIRE

    Neagoe, Catalin Andrei; Gil Espert, Lluís

    2013-01-01

    In the current report are analyzed the experimental results of initial tensile tests of Basalt FRP (BFRP) rebars provided by VSL. The investigation was conducted on the 19th of February 2013, in the laboratory of CER LITEM. Objectives of the study: to determine the tensile strengths of 6 mm and 8 mm basalt FRP rebars, and to determine what type of test setup and anchorage system is best suited for future tensile tests of BFRP bars.

  14. Image guided placement of temporary anchorage devices for tooth movement

    International Nuclear Information System (INIS)

    The aim of this project is to develop an image guided protocol for placement of a temporary anchorage device without surgically reflecting a mucoperiosteal flap. Eighteen orthodontic cases were selected for skeletal anchorage from the department of orthodontics at Case University. CBCT images of the subjects were taken using the Hitachi CB MercuRay system set at 15 mA, 120 kVp. CBCT images evaluated the ideal location for TAD placement in three dimensions. Horizontal and vertical linear measurements were taken from fixed dental landmarks to clearly define the location for placement. Transverse slices were used to evaluate the thickness of the buccal plate. Using the transverse view, the angle of insertion was determined such that the maximum buccal plate surface area would contact the screw. TADs were placed in the optimum location, with the most appropriate angle of insertion using a closed approach and with minimal local anesthesia and without flap elevation. Results: All TADs were placed without anatomic encroachment and enabled fixed orthodontic anchorage. (orig.)

  15. Image guided placement of temporary anchorage devices for tooth movement

    Energy Technology Data Exchange (ETDEWEB)

    Bahl-Palomo, L.; Bissada, N. [Case Western Reserve Univ. School of Dental Medicine, Dept. of Periodontics, Cleveland, OH (United States); Palomo, J.M.; Hans, M.G. [Case Western Reserve Univ. School of Dental Medicine, Dept. of Orthodontics, Cleveland, OH (United States)

    2007-06-15

    The aim of this project is to develop an image guided protocol for placement of a temporary anchorage device without surgically reflecting a mucoperiosteal flap. Eighteen orthodontic cases were selected for skeletal anchorage from the department of orthodontics at Case University. CBCT images of the subjects were taken using the Hitachi CB MercuRay system set at 15 mA, 120 kVp. CBCT images evaluated the ideal location for TAD placement in three dimensions. Horizontal and vertical linear measurements were taken from fixed dental landmarks to clearly define the location for placement. Transverse slices were used to evaluate the thickness of the buccal plate. Using the transverse view, the angle of insertion was determined such that the maximum buccal plate surface area would contact the screw. TADs were placed in the optimum location, with the most appropriate angle of insertion using a closed approach and with minimal local anesthesia and without flap elevation. Results: All TADs were placed without anatomic encroachment and enabled fixed orthodontic anchorage. (orig.)

  16. Optimizing Anterior En Masse Retraction with Miniscrew Anchorage

    Directory of Open Access Journals (Sweden)

    Pavankumar Janardan Vibhute

    2011-01-01

    Full Text Available In severely protrusive patients, skeletal anchorage from miniscrew is often used to avoid anchorage loss with preferred miniscrew location near centre of resistance (Cres of posterior teeth. Biomechanical requirement for directing retraction force towards Cres of posterior teeth demands the insertion of miniscrew in loose mucosa, where risk of infection and failure increases. In addition, undesirable biomechanical side effects on anterior and posterior segments may be possible in all three planes, when continuous arch sliding mechanics are installed with miniscrew anchorage. This paper describes technique of molar-stabilizing power arm (MSPA for simultaneous intrusion and retraction of anteriors with miniscrew placement at attached gingiva between 1st molar and 2nd premolar. Advantages of this technique include (i the need of miniscrews placement in loose mucosa apically near the Cres of the posterior teeth is eliminated, (ii the risk of infection and miniscrew failure is lowered since the miniscrew is placed in attached gingiva rather than the loose mucosa, and (iii by adjusting vertical length or replacing MSPA, alteration of the retraction force vector is possible in all three planes; thus, need of removal and repositioning of the miniscrew (e.g., in correction of occlusal cant can be eliminated.

  17. Optimizing anterior en masse retraction with miniscrew anchorage.

    Science.gov (United States)

    Vibhute, Pavankumar Janardan

    2011-01-01

    In severely protrusive patients, skeletal anchorage from miniscrew is often used to avoid anchorage loss with preferred miniscrew location near centre of resistance (Cres) of posterior teeth. Biomechanical requirement for directing retraction force towards Cres of posterior teeth demands the insertion of miniscrew in loose mucosa, where risk of infection and failure increases. In addition, undesirable biomechanical side effects on anterior and posterior segments may be possible in all three planes, when continuous arch sliding mechanics are installed with miniscrew anchorage. This paper describes technique of molar-stabilizing power arm (MSPA) for simultaneous intrusion and retraction of anteriors with miniscrew placement at attached gingiva between 1st molar and 2nd premolar. Advantages of this technique include (i) the need of miniscrews placement in loose mucosa apically near the Cres of the posterior teeth is eliminated, (ii) the risk of infection and miniscrew failure is lowered since the miniscrew is placed in attached gingiva rather than the loose mucosa, and (iii) by adjusting vertical length or replacing MSPA, alteration of the retraction force vector is possible in all three planes; thus, need of removal and repositioning of the miniscrew (e.g., in correction of occlusal cant) can be eliminated. PMID:22567438

  18. CFRP Mechanical Anchorage for Externally Strengthened RC Beams under Flexure

    Science.gov (United States)

    Ali, Alnadher; Abdalla, Jamal; Hawileh, Rami; Galal, Khaled

    De-bonding of carbon fiber reinforced polymers (CFRP) sheets and plates from the concrete substrate is one of the major reasons behind premature failures of beams that are externally strengthened with such CFRP materials. To delay or prevent de-bonding and therefore enhancing the load carrying capacity of strengthened beams, several anchorage systems were developed and used. This paper investigates the use of CFRP mechanical anchorage of CFRP sheets and plates used to externally strengthen reinforced concrete beams under flexure. The pin-and-fan shape CFRP anchor, which is custom-made from typical rolled fiber sheets and bundles of loose fiber is used. Several reinforced concrete beams were casted and tested in standard four-point bending scheme to study the effectiveness of this anchorage system. The beams were externally strengthened in flexure with bonded CFRP sheets and plates and then fastened to the soffit of the beams' using various patterns of CFRP anchors. It is observed that the CFRP plates begins to separate from the beams as soon as de-bonding occurs in specimens without CFRP anchors, while in beams with CFRP anchors de-bonding was delayed leading to increase in the load carrying capacity over the un-anchored strengthened beams.

  19. Cognitive Performance and Functional Competence as Predictors of Community Independence in Schizophrenia

    OpenAIRE

    Heinrichs, R. Walter; Ammari, Narmeen; Miles, Ashley A.; McDermid Vaz, Stephanie

    2008-01-01

    Measures of functional competence have been introduced to supplement standard cognitive and neuropsychological evaluations in schizophrenia research and practice. Functional competence comprises skills and abilities that are more relevant to daily life and community adjustment. However, it is unclear whether relevance translates into significantly enhanced prediction of real-world outcomes. The aim of this study was to assess the specific contribution of functional competence in predicting a ...

  20. MRI Study on the Functional and Spatial Consistency of Resting State-Related Independent Components of the Brain Network

    Energy Technology Data Exchange (ETDEWEB)

    Jeong, Bum Seok [Korea Advanced Institute of Science and Technology, Daejeon (Korea, Republic of); Choi, Jee Wook [Daejeon St. Mary' s Hospital, The Catholic University of Korea College of Medicine, Daejeon (Korea, Republic of); Kim, Ji Woong [College of Medical Science, Konyang University, Daejeon(Korea, Republic of)

    2012-06-15

    Resting-state networks (RSNs), including the default mode network (DMN), have been considered as markers of brain status such as consciousness, developmental change, and treatment effects. The consistency of functional connectivity among RSNs has not been fully explored, especially among resting-state-related independent components (RSICs). This resting-state fMRI study addressed the consistency of functional connectivity among RSICs as well as their spatial consistency between 'at day 1' and 'after 4 weeks' in 13 healthy volunteers. We found that most RSICs, especially the DMN, are reproducible across time, whereas some RSICs were variable in either their spatial characteristics or their functional connectivity. Relatively low spatial consistency was found in the basal ganglia, a parietal region of left frontoparietal network, and the supplementary motor area. The functional connectivity between two independent components, the bilateral angular/supramarginal gyri/intraparietal lobule and bilateral middle temporal/occipital gyri, was decreased across time regardless of the correlation analysis method employed, (Pearson's or partial correlation). RSICs showing variable consistency are different between spatial characteristics and functional connectivity. To understand the brain as a dynamic network, we recommend further investigation of both changes in the activation of specific regions and the modulation of functional connectivity in the brain network.

  1. Peptidyl-prolyl cis/trans isomerase-independent functional NifH mutant of Azotobacter vinelandii.

    Science.gov (United States)

    Gavini, Nara; Tungtur, Sudheer; Pulakat, Lakshmi

    2006-08-01

    Peptidyl-prolyl cis/trans isomerases (PPIases) play a pivotal role in catalyzing the correct folding of many prokaryotic and eukaryotic proteins that are implicated in a variety of biological functions, ranging from cell cycle regulation to bacterial infection. The nif accessory protein NifM, which is essential for the biogenesis of a functional NifH component of nitrogenase, is a PPIase. To understand the nature of the molecular signature that defines the NifM dependence of NifH, we screened a library of nifH mutants in the nitrogen-fixing bacterium Azotobacter vinelandii for mutants that acquired NifM independence. Here, we report that NifH can acquire NifM independence when the conserved Pro258 located in the C-terminal region of NifH, which wraps around the other subunit in the NifH dimer, is replaced by serine. PMID:16885471

  2. Internal structure of mandible around mandibular molar using computed tomography. Anatomical consideration of molar anchorage in orthodontic treatment

    International Nuclear Information System (INIS)

    For establishment of fine occlusion, facial profile and oral function in orthodontic treatment, molar anchorage in teeth movement is important manner in extracted cases. The aims of this study were to investigate the relationship between facial morphology and internal structure of mandibular body in molar region by computer tomography, and to discuss about molar anchorage in orthodontic treatment. The data for this study were obtained from 35 modern male Japanese skulls (mean age; 27 year-old, ranged from 18 year-old to 47 year-old). Measurement variables were FMA, SN to mandibular plane angle, gonial angle, alveolar breadth, cortical bone thickness of buccal and lingual sides, and the distance between dental root and cortical bone. As a result, alveolar breadth and the distance between dental root and cortical bone were narrow in long facial type, on the contrary, these variables were wide in short facial type. The result suggested that these variables were considered important factors as molar anchorage in extracted cases. (author)

  3. IFNγ inhibits Th17 differentiation and function via Tbet-dependent and Tbet-independent mechanisms

    Science.gov (United States)

    Yeh, Wen-I; McWilliams, Ian L.; Harrington, Laurie E.

    2015-01-01

    The transcription factor Tbet is critical for the differentiation of Th1 CD4 T cells and is associated with the induction of multiple autoimmune diseases, including experimental autoimmune encephalomyelitis (EAE). Herein, we demonstrate that Tbet suppresses IL-17A and Th17 differentiation both in vitro and in vivo in a cell-intrinsic manner, and that in fact, Tbet is not necessary for EAE induction. Moreover, we find that IFNγ inhibits the production of IL-17A and IL-17F in a STAT1-dependent, Tbet-independent manner. These findings illustrate multiple mechanisms utilized by developing Th1 cells to silence the Th17 program. PMID:24369297

  4. Automatic detection of local arterial input functions through Independent Component Analysis on Dynamic Contrast enhanced Magnetic Resonance Imaging.

    Science.gov (United States)

    Narvaez, Mario; Ruiz-Espana, Silvia; Arana, Estanislao; Moratal, David

    2015-08-01

    Arterial Input Function (AIF) is obtained from perfusion studies as a basic parameter for the calculus of hemodynamic variables used as surrogate markers of the vascular status of tissues. However, at present, its identification is made manually leading to high subjectivity, low repeatability and considerable time consumption. We propose an alternative method to automatically identify local AIF in perfusion images using Independent Component Analysis. PMID:26737244

  5. Extracting Intrinsic Functional Networks with Feature-Based Group Independent Component Analysis

    Science.gov (United States)

    Calhoun, Vince D.; Allen, Elena

    2013-01-01

    There is increasing use of functional imaging data to understand the macro-connectome of the human brain. Of particular interest is the structure and function of intrinsic networks (regions exhibiting temporally coherent activity both at rest and while a task is being performed), which account for a significant portion of the variance in…

  6. Mini-Implants in the Anchorage Armamentarium: New Paradigms in the Orthodontics

    OpenAIRE

    Masaru Yamaguchi; Toshihiro Inami; Ko Ito; Kazutaka Kasai; Yasuhiro Tanimoto

    2012-01-01

    Paradigms have started to shift in the orthodontic world since the introduction of mini-implants in the anchorage armamentarium. Various forms of skeletal anchorage, including miniscrews and miniplates, have been reported in the literature. Recently, great emphasis has been placed on the miniscrew type of temporary anchorage device (TAD). These devices are small, are implanted with a relatively simple surgical procedure, and increase the potential for better orthodontic results. Therefore, mi...

  7. Studies on regge behaviour and spin-independent and spin-dependent structure functions

    CERN Document Server

    Jamil, Begum Umme

    2010-01-01

    Deep Inelastic Scattering (DIS) experiments have provided important information on the structure of hadrons and ultimately the structure of matter and on the nature of interactions between leptons and hadrons, since the discovery of partons. Various high energy deep inelastic interactions lead to different evolution equations from which we obtain various structure functions giving information about the partons i.e. quarks and gluons involved in different scattering processes. Actually structure function is a mathematical picture of the hadron structure in the high energy region. Understanding the behaviour of the structure functions of the nucleon at low-x, where x is the Bjorken variable, is interesting both theoretically and phenomenologically. Structure functions are important inputs in many high energy processes and also important for examination of perturbative quantum chromodynamics (PQCD), the underlying dynamics of quarks and gluons. In PQCD, for high-Q2, where Q2 is the four momentum transfer in a DI...

  8. The probability distribution function for the sum of squares of independent random variables

    Science.gov (United States)

    Fateev, Yury; Dmitriev, Dmitry; Tyapkin, Valery; Kremez, Nikolai; Shaidurov, Vladimir

    2016-08-01

    In the present paper, the probability distribution function is derived for the sum of squares of random variables for nonzero expectations. This distribution function enables one to develop an efficient one-step algorithm for phase ambiguity resolution when determining the spatial orientation from signals of satellite radio-navigation systems. Threshold values for rejecting false solutions and statistical properties of the algorithm are obtained.

  9. A layered searchable encryption scheme with functional components independent of encryption methods.

    Science.gov (United States)

    Luo, Guangchun; Peng, Ningduo; Qin, Ke; Chen, Aiguo

    2014-01-01

    Searchable encryption technique enables the users to securely store and search their documents over the remote semitrusted server, which is especially suitable for protecting sensitive data in the cloud. However, various settings (based on symmetric or asymmetric encryption) and functionalities (ranked keyword query, range query, phrase query, etc.) are often realized by different methods with different searchable structures that are generally not compatible with each other, which limits the scope of application and hinders the functional extensions. We prove that asymmetric searchable structure could be converted to symmetric structure, and functions could be modeled separately apart from the core searchable structure. Based on this observation, we propose a layered searchable encryption (LSE) scheme, which provides compatibility, flexibility, and security for various settings and functionalities. In this scheme, the outputs of the core searchable component based on either symmetric or asymmetric setting are converted to some uniform mappings, which are then transmitted to loosely coupled functional components to further filter the results. In such a way, all functional components could directly support both symmetric and asymmetric settings. Based on LSE, we propose two representative and novel constructions for ranked keyword query (previously only available in symmetric scheme) and range query (previously only available in asymmetric scheme).

  10. Independent Component Analysis of Functional Magnetic Resonance Imaging (fMRI Data: A simple Approach

    Directory of Open Access Journals (Sweden)

    Jawad A. Shah

    2013-05-01

    Full Text Available Independent Component Analysis (ICA separates spatial and temporal components of fMRI data which may consist of activation patterns, cardiac and respiratory tasks and other artifacts. In this study sources of (fMRI data are separated using ICA based on simple fixed point iteration method and steepest ascent method. Both are the simplest methods used in optimization. However in this study complete matrix W (un-mixing matrix is updated in each iteration instead of vector based updating of W. This makes the source separation process very fast. Simulated fMRI data is processed using the proposed method and the results are compared with other ICA approaches in terms of speed and accuracy.

  11. Independent component analysis of high-resolution imaging data identifies distinct functional domains

    DEFF Research Database (Denmark)

    Reidl, Juergen; Starke, Jens; Omer, David;

    2007-01-01

    . Here we demonstrate that principal component analysis (PCA) followed by spatial independent component analysis (sICA), can be exploited to reduce the dimensionality of data sets recorded in the olfactory bulb and the somatosensory cortex of mice as well as the visual cortex of monkeys, without loosing...... the stimulus specific responses. Different neuronal populations are separated based on their stimulus specific time courses of activation. Both, spatial and temporal response characteristics can be objectively obtained, simultaneously. In the olfactory bulb, groups of glomeruli with different response...... latencies can be identified. This is shown for recordings of olfactory receptor neuron input measured with a calcium sensitive axon tracer and for network dynamics measured with the voltage sensitive dye RH 1838. In the somatosensory cortex, barrels responding to the stimulation of single whiskers can...

  12. Independently evolved upper jaw protrusion mechanisms show convergent hydrodynamic function in teleost fishes.

    Science.gov (United States)

    Staab, Katie Lynn; Holzman, Roi; Hernandez, L Patricia; Wainwright, Peter C

    2012-05-01

    A protrusible upper jaw has independently evolved multiple times within teleosts and has been implicated in the success of two groups in particular: Acanthomorpha and Cypriniformes. We use digital particle image velocimetry (DPIV) to compare suction feeding flow dynamics in a representative of each of these clades: goldfish and bluegill. Using DPIV, we contrast the spatial pattern of flow, the temporal relationship between flow and head kinematics, and the contribution of jaw protrusion to the forces exerted on prey. As expected, the spatial patterns of flow were similar in the two species. However, goldfish were slower to reach maximal kinematic excursions, and were more flexible in the relative timing of jaw protrusion, other jaw movements and suction flows. Goldfish were also able to sustain flow speeds for a prolonged period of time as compared with bluegill, in part because goldfish generate lower peak flow speeds. In both species, jaw protrusion increased the force exerted on the prey. However, slower jaw protrusion in goldfish resulted in less augmentation of suction forces. This difference in force exerted on prey corresponds with differences in trophic niches and feeding behavior of the two species. The bluegill uses powerful suction to capture insect larvae whereas the goldfish uses winnowing to sort through detritus and sediment. The kinethmoid of goldfish may permit jaw protrusion that is independent of lower jaw movement, which could explain the ability of goldfish to decouple suction flows (due to buccal expansion) from upper jaw protrusion. Nevertheless, our results show that jaw protrusion allows both species to augment the force exerted on prey, suggesting that this is a fundamental benefit of jaw protrusion to suction feeders. PMID:22496281

  13. Model-Independent Evolution of Transverse Momentum Dependent Distribution Functions (TMDs) at NNLL

    CERN Document Server

    Echevarria, Miguel G; Schäfer, Andreas; Scimemi, Ignazio

    2012-01-01

    We present a new approach to treat the perturbative QCD evolution of TMDs by resummation. We obtain a universal evolution kernel for the eight quark distributions while large logarithms are resummed up to next-to-next-to leading logarithms (NNLL). Contrary to earlier works this resummation gives unambiguous predictions avoiding the need to introduce an ad-hoc cut-off parameters and thus highly reducing the model-dependence. Applications to single-spin asymmetries, as the Sivers function, and unpolarized transverse-momentum-dependent parton distribution functions are given. The results agree very well with phenomenology.

  14. Evaluation of Treatment in the Smart Home IRIS in terms of Functional Independence and Occupational Performance and Satisfaction

    Directory of Open Access Journals (Sweden)

    Julija Ocepek

    2013-01-01

    Full Text Available The development of assistive technologies, home modifications, and smart homes has rapidly advanced in the last two decades. Health professionals have recognised the benefits of these technologies in improving individual’s quality of life. The Smart Home IRIS was established in 2008 within the University Rehabilitation Institute in Ljubljana with the aim to enable persons with disabilities and elderly people to test various assistive technologies and technical solutions for their independent living. We investigated the effect of treatments in the Smart Home IRIS. A convenience sample of 59 persons with disabilities and elderly people (aged 24–81 years who were treated in the Smart Home IRIS from April to December 2011 participated. Standardised instruments—the Canadian Occupational Performance Measure (COPM and the Functional Independence Measure (FIM—were administered at the first assessment in the Smart Home IRIS and at a second assessment at the participant’s home after 6–12 months. All the outcomes statistically significantly improved from the first to the second assessment. The treatments in the Smart Home IRIS appeared to contribute to higher occupational performance and satisfaction with performance and higher functional independence of persons with disabilities and elderly people.

  15. Evaluation of treatment in the Smart Home IRIS in terms of functional independence and occupational performance and satisfaction.

    Science.gov (United States)

    Ocepek, Julija; Roberts, Anne E K; Vidmar, Gaj

    2013-01-01

    The development of assistive technologies, home modifications, and smart homes has rapidly advanced in the last two decades. Health professionals have recognised the benefits of these technologies in improving individual's quality of life. The Smart Home IRIS was established in 2008 within the University Rehabilitation Institute in Ljubljana with the aim to enable persons with disabilities and elderly people to test various assistive technologies and technical solutions for their independent living. We investigated the effect of treatments in the Smart Home IRIS. A convenience sample of 59 persons with disabilities and elderly people (aged 24-81 years) who were treated in the Smart Home IRIS from April to December 2011 participated. Standardised instruments--the Canadian Occupational Performance Measure (COPM) and the Functional Independence Measure (FIM)--were administered at the first assessment in the Smart Home IRIS and at a second assessment at the participant's home after 6-12 months. All the outcomes statistically significantly improved from the first to the second assessment. The treatments in the Smart Home IRIS appeared to contribute to higher occupational performance and satisfaction with performance and higher functional independence of persons with disabilities and elderly people.

  16. Identification of Distinct Breast Cancer Stem Cell Populations Based on Single-Cell Analyses of Functionally Enriched Stem and Progenitor Pools

    OpenAIRE

    Nina Akrap; Daniel Andersson; Eva Bom; Pernilla Gregersson; Anders Ståhlberg; Göran Landberg

    2016-01-01

    Summary The identification of breast cancer cell subpopulations featuring truly malignant stem cell qualities is a challenge due to the complexity of the disease and lack of general markers. By combining extensive single-cell gene expression profiling with three functional strategies for cancer stem cell enrichment including anchorage-independent culture, hypoxia, and analyses of low-proliferative, label-retaining cells derived from mammospheres, we identified distinct stem cell clusters in b...

  17. Components of Intraflagellar Transport Complex A Function Independently of the Cilium to Regulate Canonical Wnt Signaling in Drosophila.

    Science.gov (United States)

    Balmer, Sophie; Dussert, Aurore; Collu, Giovanna M; Benitez, Elvira; Iomini, Carlo; Mlodzik, Marek

    2015-09-28

    The development of multicellular organisms requires the precisely coordinated regulation of an evolutionarily conserved group of signaling pathways. Temporal and spatial control of these signaling cascades is achieved through networks of regulatory proteins, segregation of pathway components in specific subcellular compartments, or both. In vertebrates, dysregulation of primary cilia function has been strongly linked to developmental signaling defects, yet it remains unclear whether cilia sequester pathway components to regulate their activation or cilia-associated proteins directly modulate developmental signaling events. To elucidate this question, we conducted an RNAi-based screen in Drosophila non-ciliated cells to test for cilium-independent loss-of-function phenotypes of ciliary proteins in developmental signaling pathways. Our results show no effect on Hedgehog signaling. In contrast, our screen identified several cilia-associated proteins as functioning in canonical Wnt signaling. Further characterization of specific components of Intraflagellar Transport complex A uncovered a cilia-independent function in potentiating Wnt signals by promoting β-catenin/Armadillo activity. PMID:26364750

  18. Model-independent plotting of the cosmological scale factor as a function of lookback time

    International Nuclear Information System (INIS)

    In this work we describe a model-independent method of developing a plot of scale factor a(t) versus lookback time tL from the usual Hubble diagram of modulus data against redshift. This is the first plot of this type. We follow the model-independent methodology of Daly and Djorgovski used for their radio-galaxy data. Once the a(t)data plot is completed, any model can be applied and will display as described in the standard literature. We then compile an extensive data set to z = 1.8 by combining Type Ia supernovae (SNe Ia) data from SNLS3 of Conley et al., high-z SNe data of Riess et al., and radio-galaxy data of Daly and Djorgovski to validate the new plot. We first display these data on a standard Hubble diagram to confirm the best fit for ΛCDM cosmology, and thus validate the joined data set. The scale factor plot is then developed from the data and the ΛCDM model is again displayed from a least-squares fit. The fit parameters are in agreement with the Hubble diagram fit confirming the validity of the new plot. Of special interest is the transition time of the universe, which in the scale factor plot will appear as an inflection point in the data set. Noise is more visible in this presentation, which is particularly sensitive to inflection points of any model displayed in the plot, unlike on a modulus-z diagram, where there are no inflection points and the transition-z is not at all obvious by inspection. We obtain a lower limit of z ≥ 0.6. It is evident from this presentation that there is a dearth of SNe data in the range z = 1-2, exactly the range necessary to confirm a ΛCDM transition-z around z = 0.76. We then compare a 'toy model' wherein dark matter is represented as a perfect fluid with an equation of state p = –(1/3) ρ to demonstrate the plot sensitivity to model choice. Its density varies as 1/t 2 and it enters the Friedmann equations as Ωdark/t 2, replacing only the Ωdark/a 3 term. The toy model is a close match to ΛCDM, but

  19. Human regulatory T cell suppressive function is independent of apoptosis induction in activated effector T cells.

    Directory of Open Access Journals (Sweden)

    Yvonne Vercoulen

    Full Text Available BACKGROUND: CD4(+CD25(+FOXP3(+ Regulatory T cells (Treg play a central role in the immune balance to prevent autoimmune disease. One outstanding question is how Tregs suppress effector immune responses in human. Experiments in mice demonstrated that Treg restrict effector T cell (Teff responses by deprivation of the growth factor IL-2 through Treg consumption, resulting in apoptosis of Teff. PRINCIPAL FINDINGS: In this study we investigated the relevance of Teff apoptosis induction to human Treg function. To this end, we studied naturally occurring Treg (nTreg from peripheral blood of healthy donors, and, to investigate Treg function in inflammation in vivo, Treg from synovial fluid of Juvenile Idiopathic Arthritis (JIA patients (SF-Treg. Both nTreg and SF-Treg suppress Teff proliferation and cytokine production efficiently as predicted. However, in contrast with murine Treg, neither nTreg nor SF-Treg induce apoptosis in Teff. Furthermore, exogenously supplied IL-2 and IL-7 reverse suppression, but do not influence apoptosis of Teff. SIGNIFICANCE: Our functional data here support that Treg are excellent clinical targets to counteract autoimmune diseases. For optimal functional outcome in human clinical trials, future work should focus on the ability of Treg to suppress proliferation and cytokine production of Teff, rather than induction of Teff apoptosis.

  20. Successful aging as a continuum of functional independence: lessons from physical disability models of aging.

    NARCIS (Netherlands)

    Lowry, K.A.; Vallejo, A.N.; Studenski, S.A.

    2012-01-01

    Successful aging is a multidimensional construct that could be viewed as a continuum of achievement. Based on the disability model proposed by the WHO International Classification of Functioning, Disability and Health, successful aging includes not only the presence or absence of disease, but also a

  1. Beyond an Autism Diagnosis: Children's Functional Independence and Parents' Unmet Needs

    Science.gov (United States)

    Brown, Hilary K.; Ouellette-Kuntz, Helene; Hunter, Duncan; Kelley, Elizabeth; Cobigo, Virginie; Lam, Miu

    2011-01-01

    High demand has resulted in gaps in autism service provision. Our objective was to explore the association between children's functioning and parents' perceived unmet needs. We conducted a cross-sectional study of 97 families of school-aged children with an autism spectrum disorder. Log binomial regression was used to examine the relative risk for…

  2. Inflammasome-Mediated Inhibition of Listeria monocytogenes-Stimulated Immunity Is Independent of Myelomonocytic Function

    Science.gov (United States)

    Williams, Cassandra R.; Dustin, Michael L.; Sauer, John-Demian

    2013-01-01

    Activation of the Nlrc4 inflammasome results in the secretion of IL-1β and IL-18 through caspase-1 and induction of pyroptosis. L. monocytogenes engineered to activate Nlrc4 by expression of Legionella pneumophilia flagellin (L. monocytogenes L.p.FlaA) are less immunogenic for CD8+ T cell responses than wt L. monocytogenes. It is also known that IL-1β orchestrates recruitment of myelomonocytic cells (MMC), which have been shown to interfere with T cell-dendritic cells (DC) interactions in splenic white pulp (WP), limiting T cell priming and protective immunity. We have further analyzed the role of MMCs in the immunogenicity of L. monocytogenes L.p.FlaA. We confirmed that MMCs infiltrate the WP between 24–48 hours in response to wt L. monocytogenes infection and that depletion of MMCs enhances CD8+ T cell priming and protective memory. L. monocytogenes L.p.FlaA elicited accelerated recruitment of MMCs into the WP. While MMCs contribute to control of L. monocytogenes L.p.FlaA, MMC depletion did not increase immunogenicity of L.p.FlaA expressing strains. There was a significant decrease in L. monocytogenes L.p.FlaA in CD8α+ DCs independent of MMCs. These findings suggest that limiting inflammasome activation is important for bacterial accumulation in CD8α+ DCs, which are known to be critical for T cell response to L. monocytogenes. PMID:24349458

  3. Inflammasome-mediated inhibition of Listeria monocytogenes-stimulated immunity is independent of myelomonocytic function.

    Directory of Open Access Journals (Sweden)

    Cassandra R Williams

    Full Text Available Activation of the Nlrc4 inflammasome results in the secretion of IL-1β and IL-18 through caspase-1 and induction of pyroptosis. L. monocytogenes engineered to activate Nlrc4 by expression of Legionella pneumophilia flagellin (L. monocytogenes L.p.FlaA are less immunogenic for CD8(+ T cell responses than wt L. monocytogenes. It is also known that IL-1β orchestrates recruitment of myelomonocytic cells (MMC, which have been shown to interfere with T cell-dendritic cells (DC interactions in splenic white pulp (WP, limiting T cell priming and protective immunity. We have further analyzed the role of MMCs in the immunogenicity of L. monocytogenes L.p.FlaA. We confirmed that MMCs infiltrate the WP between 24-48 hours in response to wt L. monocytogenes infection and that depletion of MMCs enhances CD8(+ T cell priming and protective memory. L. monocytogenes L.p.FlaA elicited accelerated recruitment of MMCs into the WP. While MMCs contribute to control of L. monocytogenes L.p.FlaA, MMC depletion did not increase immunogenicity of L.p.FlaA expressing strains. There was a significant decrease in L. monocytogenes L.p.FlaA in CD8α(+ DCs independent of MMCs. These findings suggest that limiting inflammasome activation is important for bacterial accumulation in CD8α(+ DCs, which are known to be critical for T cell response to L. monocytogenes.

  4. Comparative photoelastic study of dental and skeletal anchorages in the canine retraction

    Directory of Open Access Journals (Sweden)

    Cristiane Aparecida de Assis Claro

    2014-01-01

    Full Text Available OBJECTIVE: To compare dental and skeletal anchorages in mandibular canine retraction by means of a stress distribution analysis. METHODS: A photoelastic model was produced from second molar to canine, without the first premolar, and mandibular canine retraction was simulated by a rubber band tied to two types of anchorage: dental anchorage, in the first molar attached to adjacent teeth, and skeletal anchorage with a hook simulating the mini-implant. The forces were applied 10 times and observed in a circular polariscope. The stresses located in the mandibular canine were recorded in 7 regions. The Mann-Whitney test was employed to compare the stress in each region and between both anchorage systems. The stresses in the mandibular canine periradicular regions were compared by the Kruskal-Wallis test. RESULTS: Stresses were similar in the cervical region and the middle third. In the apical third, the stresses associated with skeletal anchorage were higher than the stresses associated with dental anchorage. The results of the Kruskal-Wallis test showed that the highest stresses were identified in the cervical-distal, apical-distal, and apex regions with the use of dental anchorage, and in the apical-distal, apical-mesial, cervical-distal, and apex regions with the use of skeletal anchorage. CONCLUSIONS: The use of skeletal anchorage in canine retraction caused greater stress in the apical third than the use of dental anchorage, which indicates an intrusive component resulting from the direction of the force due to the position of the mini-implant and the bracket hook of the canine.

  5. Application of independent component analysis for speech-music separation using an efficient score function estimation

    Science.gov (United States)

    Pishravian, Arash; Aghabozorgi Sahaf, Masoud Reza

    2012-12-01

    In this paper speech-music separation using Blind Source Separation is discussed. The separating algorithm is based on the mutual information minimization where the natural gradient algorithm is used for minimization. In order to do that, score function estimation from observation signals (combination of speech and music) samples is needed. The accuracy and the speed of the mentioned estimation will affect on the quality of the separated signals and the processing time of the algorithm. The score function estimation in the presented algorithm is based on Gaussian mixture based kernel density estimation method. The experimental results of the presented algorithm on the speech-music separation and comparing to the separating algorithm which is based on the Minimum Mean Square Error estimator, indicate that it can cause better performance and less processing time

  6. Does Sensory Function Decline Independently or Concomitantly with Age? Data from the Baltimore Longitudinal Study of Aging

    Science.gov (United States)

    Gadkaree, Shekhar K.; Sun, Daniel Q.; Li, Carol; Lin, Frank R.; Ferrucci, Luigi; Simonsick, Eleanor M.

    2016-01-01

    Objectives. To investigate whether sensory function declines independently or in parallel with age within a single individual. Methods. Cross-sectional analysis of Baltimore Longitudinal Study of Aging (BLSA) participants who underwent vision (visual acuity threshold), proprioception (ankle joint proprioceptive threshold), vestibular function (cervical vestibular-evoked myogenic potential), hearing (pure-tone average audiometric threshold), and Health ABC physical performance battery testing. Results. A total of 276 participants (mean age 70 years, range 26–93) underwent all four sensory tests. The function of all four systems declined with age. After age adjustment, there were no significant associations between sensory systems. Among 70–79-year-olds, dual or triple sensory impairment was associated with poorer physical performance. Discussion. Our findings suggest that beyond the common mechanism of aging, other distinct (nonshared) etiologic mechanisms may contribute to decline in each sensory system. Multiple sensory impairments influence physical performance among individuals in middle old-age (age 70–79).

  7. The Effect of Insulin Signaling on Female Reproductive Function Independent of Adiposity and Hyperglycemia

    OpenAIRE

    Nandi, Anindita; Wang, Xiangyuan; Accili, Domenico; Wolgemuth, Debra J.

    2010-01-01

    Physiological states of insulin resistance such as obesity and diabetes have been linked to abnormalities in female reproductive function. However, it is difficult to distinguish the direct effects of impaired insulin signaling from those of adiposity or hyperglycemia because these conditions often coexist in human syndromes and animal models of insulin resistance. In this study, we used lean, normoglycemic mouse lines with differing degrees of hyperinsulinemia and insulin receptor (Insr) exp...

  8. CT Air Trapping Is Independently Associated with Lung Function Reduction over Time

    OpenAIRE

    Mets, O.M.; de Jong, P. A.; van Ginneken, B.; Kruitwagen, C.L.J.J.; Prokop, M; Oudkerk, M; Lammers, J. W.; Zanen, P.

    2013-01-01

    Purpose We aimed to study the association between lung function decline and quantitative computed tomography (CT) air trapping. Materials and Methods Current and former heavy smokers in a lung cancer screening trial underwent volumetric low-dose CT in inspiration and expiration. Spirometry was obtained at baseline and after 3 years. The expiratory to inspiratory ratio of mean lung density (E/I-ratioMLD) was used to quantify air trapping. CT emphysema was defined as voxels in inspiratory CT be...

  9. Magnetic measurement and analysis of independent and combined function steering magnets for Indus-2

    International Nuclear Information System (INIS)

    Indus-2 is a 2.5 GeV synchrotron light source under construction at CAT, Indore. Storage ring consists of a total 16 dipole, 72 quadrupole, 32 sextupole and 56 steering magnets. Out of these 56 steering magnets, 32 are combined function magnets, 16 horizontal and 8 vertical steering magnets. Results of magnetic measurements of steering magnets are discussed in this paper. (author)

  10. Brd4-Independent Transcriptional Repression Function of the Papillomavirus E2 Proteins▿

    OpenAIRE

    Schweiger, Michal-Ruth; Ottinger, Matthias; You, Jianxin; Howley, Peter M.

    2007-01-01

    The papillomavirus E2 protein is a critical viral regulatory protein with transcription, DNA replication, and genome maintenance functions. We have previously identified the cellular bromodomain protein Brd4 as a major E2-interacting protein and established that it participates in tethering bovine papillomavirus type 1 E2 and viral genomes to host cell mitotic chromosomes. We have also shown that Brd4 mediates E2-dependent transcriptional activation, which is strongly inhibited by the disrupt...

  11. Speech Discrimination Difficulties in High-Functioning Autism Spectrum Disorder Are Likely Independent of Auditory Hypersensitivity.

    Science.gov (United States)

    Dunlop, William A; Enticott, Peter G; Rajan, Ramesh

    2016-01-01

    Autism Spectrum Disorder (ASD), characterized by impaired communication skills and repetitive behaviors, can also result in differences in sensory perception. Individuals with ASD often perform normally in simple auditory tasks but poorly compared to typically developed (TD) individuals on complex auditory tasks like discriminating speech from complex background noise. A common trait of individuals with ASD is hypersensitivity to auditory stimulation. No studies to our knowledge consider whether hypersensitivity to sounds is related to differences in speech-in-noise discrimination. We provide novel evidence that individuals with high-functioning ASD show poor performance compared to TD individuals in a speech-in-noise discrimination task with an attentionally demanding background noise, but not in a purely energetic noise. Further, we demonstrate in our small sample that speech-hypersensitivity does not appear to predict performance in the speech-in-noise task. The findings support the argument that an attentional deficit, rather than a perceptual deficit, affects the ability of individuals with ASD to discriminate speech from background noise. Finally, we piloted a novel questionnaire that measures difficulty hearing in noisy environments, and sensitivity to non-verbal and verbal sounds. Psychometric analysis using 128 TD participants provided novel evidence for a difference in sensitivity to non-verbal and verbal sounds, and these findings were reinforced by participants with ASD who also completed the questionnaire. The study was limited by a small and high-functioning sample of participants with ASD. Future work could test larger sample sizes and include lower-functioning ASD participants. PMID:27555814

  12. Speech discrimination difficulties in High-Functioning Autism Spectrum Disorder are likely independent of auditory hypersensitivity.

    Directory of Open Access Journals (Sweden)

    William Andrew Dunlop

    2016-08-01

    Full Text Available Autism Spectrum Disorder (ASD, characterised by impaired communication skills and repetitive behaviours, can also result in differences in sensory perception. Individuals with ASD often perform normally in simple auditory tasks but poorly compared to typically developed (TD individuals on complex auditory tasks like discriminating speech from complex background noise. A common trait of individuals with ASD is hypersensitivity to auditory stimulation. No studies to our knowledge consider whether hypersensitivity to sounds is related to differences in speech-in-noise discrimination. We provide novel evidence that individuals with high-functioning ASD show poor performance compared to TD individuals in a speech-in-noise discrimination task with an attentionally demanding background noise, but not in a purely energetic noise. Further, we demonstrate in our small sample that speech-hypersensitivity does not appear to predict performance in the speech-in-noise task. The findings support the argument that an attentional deficit, rather than a perceptual deficit, affects the ability of individuals with ASD to discriminate speech from background noise. Finally, we piloted a novel questionnaire that measures difficulty hearing in noisy environments, and sensitivity to non-verbal and verbal sounds. Psychometric analysis using 128 TD participants provided novel evidence for a difference in sensitivity to non-verbal and verbal sounds, and these findings were reinforced by participants with ASD who also completed the questionnaire. The study was limited by a small and high-functioning sample of participants with ASD. Future work could test larger sample sizes and include lower-functioning ASD participants.

  13. Oxidative Stress in Hypertensive Patients Induces an Increased Contractility in Vein Grafts Independent of Endothelial Function

    Directory of Open Access Journals (Sweden)

    Claudio Joo Turoni

    2011-01-01

    Full Text Available Objective. To evaluate the impact of oxidative stress on vascular reactivity to vasoconstrictors and on nitric oxide (NO bioavailability in saphenous vein (SV graft with endothelial dysfunction from hypertensive patients (HT. Methods. Endothelial function, vascular reactivity, oxidative state, nitrites and NO release were studied in isolated SV rings from HT and normotensive patients (NT. Only rings with endothelial dysfunction were used. Results. HT rings presented a hyperreactivity to vasoconstrictors that was reverted by diphenylene iodonium (DPI. In NT, no effect of DPI was obtained, but Nω-nitro-L-arginine methyl ester (L-NAME increased the contractile response. NO was present in SV rings without endothelial function. Nitrites were higher in NT than in HT (1066.1 ± 86.3 pmol/mg; n=11 versus 487.8 ± 51.6; n=23; P<0.01 and inhibited by nNOS inhibitor. L-arginine reversed this effect. Antioxidant agents increased nitrites and NO contents only in HT. The anti-nNOS-stained area by immunohistochemistry was higher in NT than HT. HT showed an elevation of oxidative state. Conclusions. Extraendothelial NO counter-regulates contractility in SV. However, this action could be altered in hypertensive situations by an increased oxidative stress or a decreased ability of nNOS to produce NO. Further studies should be performed to evaluate the implication of these results in graft patency rates.

  14. FIT: Computer Program that Interactively Determines Polynomial Equations for Data which are a Function of Two Independent Variables

    Science.gov (United States)

    Arbuckle, P. D.; Sliwa, S. M.; Roy, M. L.; Tiffany, S. H.

    1985-01-01

    A computer program for interactively developing least-squares polynomial equations to fit user-supplied data is described. The program is characterized by the ability to compute the polynomial equations of a surface fit through data that are a function of two independent variables. The program utilizes the Langley Research Center graphics packages to display polynomial equation curves and data points, facilitating a qualitative evaluation of the effectiveness of the fit. An explanation of the fundamental principles and features of the program, as well as sample input and corresponding output, are included.

  15. Scheme-Independent Series Expansions at an Infrared Zero of the Beta Function in Asymptotically Free Gauge Theories

    CERN Document Server

    Ryttov, T A

    2016-01-01

    We consider an asymptotically free vectorial gauge theory, with gauge group $G$ and $N_f$ fermions in a representation $R$ of $G$, having an infrared (IR) zero in the beta function at $\\alpha_{IR}$. We present general formulas for scheme-independent series expansions of quantities, evaluated at $\\alpha_{IR}$, as powers of an $N_f$-dependent expansion parameter, $\\Delta_f$. First, we apply these to calculate the derivative $d\\beta/d\\alpha$ evaluated at $\\alpha_{IR}$, denoted $\\beta'_{IR}$, which is equal to the anomalous dimension of the ${\\rm Tr}(F_{\\mu\

  16. Willingness to Pay for Quality of Life Technologies to Enhance Independent Functioning Among Baby Boomers and the Elderly Adults

    OpenAIRE

    Schulz, Richard; Beach, Scott R.; Matthews, Judith T.; Courtney, Karen; Devito Dabbs, Annette; Person Mecca, Laurel; Sankey, Steadman Scott

    2013-01-01

    Purpose: We report the results of a study designed to assess whether and how much potential individual end users are willing to pay for Quality of Life Technologies (QoLTs) designed to enhance functioning and independence. Design and Methods: We carried out a web survey of a nationally representative sample of U.S. baby boomers (aged 45–64; N = 416) and older adults (aged 65 and greater, N = 114). Respondents were first instructed to assume that they needed help with kitchen activities/person...

  17. 49 CFR 571.225 - Standard No. 225; Child restraint anchorage systems.

    Science.gov (United States)

    2010-10-01

    ...) and (b) of S7 apply to the test procedures in S8. (a) Vehicle seats are adjusted to their full... systems. S1. Purpose and scope. This standard establishes requirements for child restraint anchorage... buses, and funeral coaches. S3. Definitions. Child restraint anchorage means any vehicle...

  18. 77 FR 8252 - Adequacy Status of the Anchorage, Alaska, Carbon Monoxide Maintenance Plan for Transportation...

    Science.gov (United States)

    2012-02-14

    ... existing violations, or delay timely attainment of the national ambient air quality standards. The minimum... AGENCY Adequacy Status of the Anchorage, Alaska, Carbon Monoxide Maintenance Plan for Transportation... budget (MVEB) in the Anchorage, Alaska, Carbon Monoxide (CO) Maintenance Plan, submitted by the State...

  19. Flexural Strengthening of RC Slabs with Prestressed CFRP Strips Using Different Anchorage Systems

    Directory of Open Access Journals (Sweden)

    José Sena-Cruz

    2015-10-01

    Full Text Available Externally Bonded Reinforcement (EBR technique has been widely used for flexural strengthening of concrete structures by using carbon fiber-reinforced polymers (CFRP. EBR technique offers several structural advantages when the CFRP material is prestressed. This paper presents an experimental and numerical study on reinforced (RC slabs strengthened in flexure with prestressed CFRP strips as a structural strengthening system. The strips are applied as an externally bonded reinforcement (EBR and anchored with either a mechanical or a gradient anchorage. The former foresees metallic anchorage plates fixed to the concrete substrate, while the latter is based on an accelerated epoxy resin curing followed by a segment-wise prestress force decrease at the strip ends. Both anchorage systems, in combination with different CFRP strip geometries, were subjected to static loading tests. It could be demonstrated that the composite strip’s performance is better exploited when prestressing is used, with slightly higher overall load carrying capacities for mechanical anchorages than for the gradient anchorage. The performed investigations by means of a cross-section analysis supported the experimental observation that in case a mechanical anchorage is used, progressive strip debonding changes the fully bonded configuration to an unbonded end-anchored system. The inclusion of defined debonding criteria for both the anchorage zones and free length between the anchorage regions allowed to precisely capture the ultimate loading forces.

  20. 78 FR 11097 - Artificial Island Anchorage No. 2 Partial Closure, Delaware River; Salem, NJ

    Science.gov (United States)

    2013-02-15

    ... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Artificial Island Anchorage No. 2 Partial Closure... for the safety of life on the navigable waters of the Artificial Island Anchorage. This closure is.... SUPPLEMENTARY INFORMATION: Table of Acronyms DHS Department of Homeland Security FR Federal Register NPRM...

  1. An Assessment of Hermite Function Based Approximations of Mutual Information Applied to Independent Component Analysis

    Directory of Open Access Journals (Sweden)

    Julian Sorensen

    2008-12-01

    Full Text Available At the heart of many ICA techniques is a nonparametric estimate of an information measure, usually via nonparametric density estimation, for example, kernel density estimation. While not as popular as kernel density estimators, orthogonal functions can be used for nonparametric density estimation (via a truncated series expansion whose coefficients are calculated from the observed data. While such estimators do not necessarily yield a valid density, which kernel density estimators do, they are faster to calculate than kernel density estimators, in particular for a modified version of Renyi's entropy of order 2. In this paper, we compare the performance of ICA using Hermite series based estimates of Shannon's and Renyi's mutual information, to that of Gaussian kernel based estimates. The comparisons also include ICA using the RADICAL estimate of Shannon's entropy and a FastICA estimate of neg-entropy.

  2. Respiratory-driven lung tumor motion is independent of tumor size, tumor location, and pulmonary function

    International Nuclear Information System (INIS)

    Purpose: To determine whether superior-inferior lung tumor motion is predictable by tumor size or location, or pulmonary function test results. Methods and Materials: Superior-inferior tumor motion was measured on orthogonal radiographs taken during simulation of 22 patients with inoperable lung cancer diagnosed by orthogonal radiographs. Results: The tumor size averaged 5.5±3.1 cm (range 1.5-12 cm). Seven of 11 central tumors demonstrated some motion compared with 5 of 11 peripheral tumors. Four of 5 upper lobe tumors moved compared with 8 of 17 tumors that were either middle or lower lobe lesions. The mean fourth rib motion was 7.3±3.2 mm (range 2-15). The mean FeV1 was 1.8±1.2 (range 0.55-5.33. The mean diffusing capacity of the lung for carbon monoxide was 14.0±6.5 (range 7.8-21.9). The mean total lung capacity was 6.5±1.2 (range 3.3-8.4). None of these parameters correlated with tumor motion. Although lateral tumor motion could not be consistently determined, 1 tumor moved 10 mm anterior-posteriorly. Conclusions: Lung tumors often move significantly during respiration. Tumor motion is not predictable by tumor size or location, or pulmonary function test results. Therefore, tumor motion must be measured in all patients. Measurement in three dimensions will likely be necessary to maximize the irradiated lung volumes or choose beam arrangements parallel to the major axis of motion

  3. Substrate-independent approach for the generation of functional protein resistant surfaces.

    Science.gov (United States)

    Rodriguez-Emmenegger, Cesar; Kylián, Ondrej; Houska, Milan; Brynda, Eduard; Artemenko, Anna; Kousal, Jaroslav; Alles, Aldo Bologna; Biederman, Hynek

    2011-04-11

    A new route for coating various substrates with antifouling polymer layers was developed. It consisted in deposition of an amino-rich adhesion layer by means of RF magnetron sputtering of Nylon 6,6 followed by the well-controlled, surface-initiated atom transfer radical polymerization of antifouling polymer brushes initiated by bromoisobutyrate covalently attached to amino groups present in the adhesion layer. Polymer brushes of hydroxy- and methoxy-capped oligoethyleneglycol methacrylate and carboxybetaine acrylamide were grafted from bromoisobutyrate initiator attached to a 15 nm thick amino-rich adhesion layer deposited on gold, silicon, polypropylene, and titanium-aluminum-vanadium alloy surfaces. Well-controlled polymerization kinetics made it possible to control the thickness of the brushes at a nanometer scale. Zero fouling from single protein solutions and a reduction of more than 90% in the fouling from blood plasma observed on the uncoated surfaces was achieved. The feasibility of functionalization with bioactive compounds was tested by covalent attachment of streptavidin onto poly(oligoethylene glycol methacrylate) brush and subsequent immobilization of model antibodies and oligonucleotides. The procedure is nondestructive and does not require any chemical preactivation or the presence of reactive groups on the substrate surface. Contrary to current antifouling modifications, the developed coating can be built on various classes of substrates and preserves its antifouling properties even in undiluted blood plasma. The new technique might be used for fabrication of biotechnological and biomedical devices with tailor-made functions that will not be impaired by fouling from ambient biological media. PMID:21381652

  4. Mini-Implants in the Anchorage Armamentarium: New Paradigms in the Orthodontics

    Directory of Open Access Journals (Sweden)

    Masaru Yamaguchi

    2012-01-01

    Full Text Available Paradigms have started to shift in the orthodontic world since the introduction of mini-implants in the anchorage armamentarium. Various forms of skeletal anchorage, including miniscrews and miniplates, have been reported in the literature. Recently, great emphasis has been placed on the miniscrew type of temporary anchorage device (TAD. These devices are small, are implanted with a relatively simple surgical procedure, and increase the potential for better orthodontic results. Therefore, miniscrews not only free orthodontists from anchorage-demanding cases, but they also enable clinicians to have good control over tooth movement in 3 dimensions. The miniplate type also produces significant improvements in treatment outcomes and has widened the spectrum of orthodontics. The purpose of this paper is to update clinicians on the current concepts and versatile uses and clinical applications of skeletal anchorage in orthodontics.

  5. Longer telomere length in COPD patients with α1-antitrypsin deficiency independent of lung function.

    Directory of Open Access Journals (Sweden)

    Aabida Saferali

    Full Text Available Oxidative stress is involved in the pathogenesis of airway obstruction in α1-antitrypsin deficient patients. This may result in a shortening of telomere length, resulting in cellular senescence. To test whether telomere length differs in α1-antitrypsin deficient patients compared with controls, we measured telomere length in DNA from peripheral blood cells of 217 α1-antitrypsin deficient patients and 217 control COPD patients. We also tested for differences in telomere length between DNA from blood and DNA from lung tissue in a subset of 51 controls. We found that telomere length in the blood was significantly longer in α1-antitrypsin deficient COPD patients compared with control COPD patients (p = 1×10(-29. Telomere length was not related to lung function in α1-antitrypsin deficient patients (p = 0.3122 or in COPD controls (p = 0.1430. Although mean telomere length was significantly shorter in the blood when compared with the lungs (p = 0.0078, telomere length was correlated between the two tissue types (p = 0.0122. Our results indicate that telomere length is better preserved in α1-antitrypsin deficient COPD patients than in non-deficient patients. In addition, measurement of telomere length in the blood may be a suitable surrogate for measurement in the lung.

  6. Longer telomere length in COPD patients with α1-antitrypsin deficiency independent of lung function.

    Science.gov (United States)

    Saferali, Aabida; Lee, Jee; Sin, Don D; Rouhani, Farshid N; Brantly, Mark L; Sandford, Andrew J

    2014-01-01

    Oxidative stress is involved in the pathogenesis of airway obstruction in α1-antitrypsin deficient patients. This may result in a shortening of telomere length, resulting in cellular senescence. To test whether telomere length differs in α1-antitrypsin deficient patients compared with controls, we measured telomere length in DNA from peripheral blood cells of 217 α1-antitrypsin deficient patients and 217 control COPD patients. We also tested for differences in telomere length between DNA from blood and DNA from lung tissue in a subset of 51 controls. We found that telomere length in the blood was significantly longer in α1-antitrypsin deficient COPD patients compared with control COPD patients (p = 1×10(-29)). Telomere length was not related to lung function in α1-antitrypsin deficient patients (p = 0.3122) or in COPD controls (p = 0.1430). Although mean telomere length was significantly shorter in the blood when compared with the lungs (p = 0.0078), telomere length was correlated between the two tissue types (p = 0.0122). Our results indicate that telomere length is better preserved in α1-antitrypsin deficient COPD patients than in non-deficient patients. In addition, measurement of telomere length in the blood may be a suitable surrogate for measurement in the lung.

  7. Treatment of a Class II deepbite with microimplant anchorage.

    Science.gov (United States)

    Park, Hyo-Sang; Kim, Ji-Yeun; Kwon, Tae-Geon

    2011-03-01

    The goal of this report was to illustrate new treatment mechanics for using microimplants for the treatment of a Class II Division 2 deepbite malocclusion. A 29-year-old woman with a deepbite was treated with the aid of microimplant anchorage. Microimplants placed between the maxillary second premolars and first molars were used as anchorage to apply a distal force to the anterior teeth to correct the Class II canine and molar relationships. A distal force was applied to long hooks that were crimped between the lateral incisors and the canines. By applying a backward force to the long hooks, the maxillary anterior teeth experienced palatal root movement with no change in the vertical and anteroposterior positions of the incisal edges. The distal extrusive movement of the maxillary second molars achieved by disengaging the second molars from the archwire during distal force application and an anterior bite-block bonded on the lingual surface of the maxillary central incisors produced the increase in vertical dimension. The distal force to the long extended hooks from the microimplants was possibly good mechanics for obtaining the palatal root movement and correcting the Class II canine and molar relationships. The anterior bite-block and disengagement of the maxillary second molars during distal force application were effective for increasing the vertical dimension.

  8. miR-422a is an independent prognostic factor and functions as a potential tumor suppressor in colorectal cancer

    Science.gov (United States)

    Zheng, Gui-Xi; Qu, Ai-Lin; Yang, Yong-Mei; Zhang, Xin; Zhang, Shou-Cai; Wang, Chuan-Xin

    2016-01-01

    AIM: To determine the expression of miR-422a in colorectal cancer (CRC) tissues and to further explore the prognostic value and function of miR-422a in CRC carcinogenesis. METHODS: miR-422a expression was analyzed in 102 CRC tissues and paired normal mucosa adjacent to carcinoma by quantitative real-time PCR. The relationship of miR-422a expression with clinicopathological parameters was also analyzed. Kaplan-Meier analysis and Cox multivariate analysis were performed to estimate the potential role of miR-422a. Cell proliferation, migration, and invasion were used for in vitro functional analysis of miR-422a. RESULTS: The levels of miR-422a were dramatically reduced in CRC tissues compared with normal mucosa (P < 0.05), and significantly correlated with local invasion (P = 0.004) and lymph node metastasis (P < 0.001). Kaplan-Meier survival and Cox regression multivariate analyses revealed that miR-422a expression (HR = 0.568, P = 0.015) and clinical TNM stage (HR = 2.942, P = 0.003) were independent prognostic factors for overall survival in CRC patients. Furthermore, in vitro experiments showed that overexpression of miR-422a inhibited the proliferation, migration, and invasion of SW480 and HT-29 cells. CONCLUSION: Down-regulation of miR-422a may serve as an independent prognosis factor in CRC. MiR-422a functions as a tumor suppressor and regulates progression of CRC. PMID:27350737

  9. 1964 Great Alaska Earthquake: a photographic tour of Anchorage, Alaska

    Science.gov (United States)

    Thoms, Evan E.; Haeussler, Peter J.; Anderson, Rebecca D.; McGimsey, Robert G.

    2014-01-01

    On March 27, 1964, at 5:36 p.m., a magnitude 9.2 earthquake, the largest recorded earthquake in U.S. history, struck southcentral Alaska (fig. 1). The Great Alaska Earthquake (also known as the Good Friday Earthquake) occurred at a pivotal time in the history of earth science, and helped lead to the acceptance of plate tectonic theory (Cox, 1973; Brocher and others, 2014). All large subduction zone earthquakes are understood through insights learned from the 1964 event, and observations and interpretations of the earthquake have influenced the design of infrastructure and seismic monitoring systems now in place. The earthquake caused extensive damage across the State, and triggered local tsunamis that devastated the Alaskan towns of Whittier, Valdez, and Seward. In Anchorage, the main cause of damage was ground shaking, which lasted approximately 4.5 minutes. Many buildings could not withstand this motion and were damaged or collapsed even though their foundations remained intact. More significantly, ground shaking triggered a number of landslides along coastal and drainage valley bluffs underlain by the Bootlegger Cove Formation, a composite of facies containing variably mixed gravel, sand, silt, and clay which were deposited over much of upper Cook Inlet during the Late Pleistocene (Ulery and others, 1983). Cyclic (or strain) softening of the more sensitive clay facies caused overlying blocks of soil to slide sideways along surfaces dipping by only a few degrees. This guide is the document version of an interactive web map that was created as part of the commemoration events for the 50th anniversary of the 1964 Great Alaska Earthquake. It is accessible at the U.S. Geological Survey (USGS) Alaska Science Center website: http://alaska.usgs.gov/announcements/news/1964Earthquake/. The website features a map display with suggested tour stops in Anchorage, historical photographs taken shortly after the earthquake, repeat photography of selected sites, scanned documents

  10. A γ-Secretase Independent Role for Presenilin in Calcium Homeostasis Impacts Mitochondrial Function and Morphology in Caenorhabditis elegans.

    Science.gov (United States)

    Sarasija, Shaarika; Norman, Kenneth R

    2015-12-01

    Mutations in the presenilin (PSEN) encoding genes (PSEN1 and PSEN2) occur in most early onset familial Alzheimer's Disease. Despite the identification of the involvement of PSEN in Alzheimer's Disease (AD) ∼20 years ago, the underlying role of PSEN in AD is not fully understood. To gain insight into the biological function of PSEN, we investigated the role of the PSEN homolog SEL-12 in Caenorhabditis elegans. Using genetic, cell biological, and pharmacological approaches, we demonstrate that mutations in sel-12 result in defects in calcium homeostasis, leading to mitochondrial dysfunction. Moreover, consistent with mammalian PSEN, we provide evidence that SEL-12 has a critical role in mediating endoplasmic reticulum (ER) calcium release. Furthermore, we found that in SEL-12-deficient animals, calcium transfer from the ER to the mitochondria leads to fragmentation of the mitochondria and mitochondrial dysfunction. Additionally, we show that the impact that SEL-12 has on mitochondrial function is independent of its role in Notch signaling, γ-secretase proteolytic activity, and amyloid plaques. Our results reveal a critical role for PSEN in mediating mitochondrial function by regulating calcium transfer from the ER to the mitochondria.

  11. Preliminary investigation of sequence-independent DNA binding proteins in rat skeletal muscle sarcoplasmic reticulum and their function

    Institute of Scientific and Technical Information of China (English)

    赵文; 姜志胜; 倪菊华; 陈光慧; 刘乃奎; 汤健; 贾弘褆; 唐朝枢

    2000-01-01

    To observe the binding of plasmid DNA to non-nuclear DNA binding proteins in sar-coplasmic reticulum (SR) and the effects of this binding on SR function, sarcoplasmic reticulum proteins in rat skeletal muscle were isolated by differential centrifuge and sucrose density-gradient centrifuge. The results showed that there are two sequence-independent DNA binding proteins in SR proteins, the molecular weights of which are 83 and 58 ku, respectively. Ca2+ uptake and release of SR were remarkably promoted by the binding of plasmid DNA to DNA binding proteins in SR, the mechanism is probably through increasing of Ca2+-ATPase activity in SR and changing of character of Ca2+ release channel ryanodine receptors induced by the binding. These results suggest that there exist DNA binding proteins in SR and its binding to DNA may affect Ca2+ transport of SR.

  12. Preliminary investigation of sequence-independent DNA binding proteins in rat skeletal muscle sarcoplasmic reticulum and their function

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    To observe the binding of plasmid DNA to non-nuclear DNA binding proteins in sarcoplasmic reticulum (SR) and the effects of this binding on SR function, sarcoplasmic reticulum proteins in rat skeletal muscle were isolated by differential centrifuge and sucrose density-gradient centrifuge. The results showed that there are two sequence-independent DNA binding proteins in SR proteins, the molecular weights of which are 83 and 58 ku, respectively. Ca2+ uptake and release of SR were remarkably promoted by the binding of plasmid DNA to DNA binding proteins in SR, the mechanism is probably through increasing of Ca2+-ATPase activity in SR and changing of character of Ca2+ release channel ryanodine receptors induced by the binding. These results suggest that there exist DNA binding proteins in SR and its binding to DNA may affect Ca2+ transport of SR.

  13. The Role of Executive Function in Independent Living Skills in Female Adolescents and Young Adults With Fragile X Syndrome.

    Science.gov (United States)

    Martin, Arianna; Quintin, Eve-Marie; Hall, Scott S; Reiss, Allan L

    2016-09-01

    Fragile X syndrome (FXS) is associated with executive function (EF) and independent living skills (ILS) deficits. We examined the role of childhood EF in ILS during adolescence/early adulthood in females with FXS and two comparison groups in the same age range (matched for IQ [IQ/Age group] and with another genetic condition, Turner syndrome [TS group]). EF and ILS were significantly higher for the FXS group than the IQ/Age group but did not differ from the TS group. For the FXS group, age and EF were significant predictors of ILS during adolescence/early adulthood, but there were no statistically significant longitudinal associations between EF and ILS. Our findings suggest that impairments in EF may have a significant effect on ILS in FXS. PMID:27611354

  14. Analysis on Bearing Capacity of Tunnel-Type Anchorage of a Long-Span Suspension Bridge

    Institute of Scientific and Technical Information of China (English)

    Zhu Yu; Wei Jun; Yang Manjuan; Li Hao; Liu Hongqing

    2005-01-01

    Due to complicated rock structure and environment, a prototype test for a tunnel-type anchorage is infeasible. Based on the rock mass parameters from tests, a three-dimensional (3D) elasto-plastic analysis was performed to simulate the influence of the construction procedure of Siduhe bridge with tunnel-type anchorage (TTA) in Hubei Province, China. The surrounding rock and concrete anchorage body were simulated by 8 nodes 3D brick elements. The geostatic state of the complex geometric structure was established with initial data. The in-situ concrete casting of the anchorage body and excavation of the rock mass were simulated by tetrahedral shell elements. The results show that the surrounding rock is in an elastic state under the designed cable force. The numerical overloading analysis indicates that the capacity of the surrounding anchorage is 7 times that of the designed cable force. The failure pattern shows that two anchorage bodies would be pulled out in the end. The maximum shear stress appears 10 m before the back anchorage face. The maximum range influenced by the TTA under ultimate loads is about 16 m.

  15. Meixner Class of Non-commutative Generalized Stochastic Processes with Freely Independent Values II. The Generating Function

    Science.gov (United States)

    Bożejko, Marek; Lytvynov, Eugene

    2011-03-01

    Let T be an underlying space with a non-atomic measure σ on it. In [ Comm. Math. Phys. 292, 99-129 (2009)] the Meixner class of non-commutative generalized stochastic processes with freely independent values, {ω=(ω(t))_{tin T}} , was characterized through the continuity of the corresponding orthogonal polynomials. In this paper, we derive a generating function for these orthogonal polynomials. The first question we have to answer is: What should serve as a generating function for a system of polynomials of infinitely many non-commuting variables? We construct a class of operator-valued functions {Z=(Z(t))_{tin T}} such that Z( t) commutes with ω( s) for any {s,tin T}. Then a generating function can be understood as {G(Z,ω)=sum_{n=0}^infty int_{T^n}P^{(n)}(ω(t_1),dots,ω(t_n))Z(t_1)dots Z(t_n)} {σ(dt_1) dots σ(dt_n)} , where {P^{(n)}(ω(t_1),dots,ω(t_n))} is (the kernel of the) n th orthogonal polynomial. We derive an explicit form of G( Z, ω), which has a resolvent form and resembles the generating function in the classical case, albeit it involves integrals of non-commuting operators. We finally discuss a related problem of the action of the annihilation operators {partial_t,t in T} . In contrast to the classical case, we prove that the operators ∂ t related to the free Gaussian and Poisson processes have a property of globality. This result is genuinely infinite-dimensional, since in one dimension one loses the notion of globality.

  16. Exterior beam-column joint study with non-conventional reinforcement detailing using mechanical anchorage under reversal loading

    Indian Academy of Sciences (India)

    S Rajagopal; S Prabavathy

    2014-10-01

    Reinforced concrete structures beam-column joints are the most critical regions in seismic prone areas. Proper reinforcement anchorage is essential to enhance the performance of the joints. An attempt has been made to appraise the performance of the anchorages and joints. The anchorages are detailed as per ACI-352 (mechanical anchorages), ACI-318 (conventional bent hooks) and IS-456 (conventional full anchorage). The joints are detailed without confinement in group-I and with additional X-cross bar in group-II. To assess the seismic performance, the specimens are assembled into two groups of three specimens each and were tested under reversal loading, The specimen with T-type mechanical anchorage (Headed bar) and T-type mechanical anchorage combination with X-cross bar exhibited significant improvement in seismic performance: load-displacement capacity, displacement ductility, stiffness degradation, controlled crack capacity in the joint shear panel and also reduced congestion of reinforcement in joint core.

  17. Possibility of Independence in ADL (Activities of Daily Living) for Patients with Cervical Spinal Cord Injuries : An Evaluation based on the Zancolli Classification of Residual Arm Functions

    OpenAIRE

    Yoshimura, Osamu; Takayanagi, Kiyomi; Kobayashi, Ryuji; Hosoda, Masataka; Minematsu, Akira; Sasaki, Hisato; Maejima, Hiroshi; Matsuda, Yuiti; Tanaka, Satiko; Matsuo, Akihisa; Kanemura, Naohiko

    1998-01-01

    For patients with cervical spinal cord injuries to become independent in their ADL (Activities of Daily Living), residual arm function is very important. Also, age, sex, physical strength, obesity, spasticity, pain, contracture and motivation are related. We investigated the possibility of independence in ADL for patients with cervical spinal cord injuries, carrying out our evaluation based on the Zancolli Classification of Residual Arm Functions. Zancolli classification C6BII is taken as the...

  18. Intrusion of overerupted molars by corticotomy and orthodontic skeletal anchorage.

    Science.gov (United States)

    Moon, Cheol-Hyun; Wee, Jin-Uk; Lee, Hyun-Sun

    2007-11-01

    This article describes the orthodontic treatment of a 26-year-old female patient with overerupted left maxillary molar teeth. Her chief complaint was that the maxillary left first and the second molar intruded into the space required for the mandibular left first and the second molars, preventing prosthodontic treatment. The authors performed a corticotomy and used orthodontic skeletal anchorage with a miniplate and orthodontic miniscrews with a head modified to provide a specially designed hook. With this approach, they were able to achieve a sufficient amount of molar intrusion without discomfort, root resorption, or extrusion of the adjacent teeth. The first molar was intruded 3.0 mm and second molar was intruded 3.5 mm during 2 months of treatment. These results have been maintained for 11 months. PMID:18004918

  19. Numerical Analysis of Quality Inspection of Anchorage System

    Institute of Scientific and Technical Information of China (English)

    ZHANGYongxing; XUMing

    2002-01-01

    Sonoprobe method has been applied in non-destructive inspection of anchorage project.The fundament is that dynamic transient excitation causes the elastic vibration of an anchor bar,and flaws can be estimated or deduced by determining transient response of the anchor bar.FEA numeric solution of hyperbolic equation indicates that deductions must comply with acoustic parameters such as velocity of sound,vibration range,wave shape etc when inspecting interior flaws in the grout of an anchor bar,Based on wavelet packet analysis,the energy eigenvector is a flaw vector,which could be regarded as the basis in the nondestructive inspection of anchors,As a non-linear dynamical system,artifical neural networks dealing with quality insection of gray system have been proved efficient.

  20. Finite element simulation and testing of ISW CFRP anchorage

    DEFF Research Database (Denmark)

    Schmidt, Jacob Wittrup; Goltermann, Per; Hertz, Kristian Dahl

    2013-01-01

    Several Carbon Fibre Reinforced Polymers (CFRP) systems have been used successfully for strengthening of structures during the last decades. However, the fracture often occurs in the concrete adherent or in the adhesive interface when used for steel strengthening. As a consequence the CFRP...... to the remaining structure. However, reaching the full capacity of the CFRP material is difficult since anchoring often courses premature failure modes such as crushing of the Fibre Reinforced Polymers (FRP), slip in the FRP and adjacent adherent, cutting of the fibres, bending of fibres and frontal overload...... is modelled in the 3D finite Element program ABAQUS, just as digital image correlation (DIC) testing was performed to verify the finite element simulation. Also a new optimized design was produced to ensure that the finite element simulation and anchorage behaviour correlated well. It is seen...

  1. Anchorage Failure of RC beams strengthened with FRP at the bottom face

    DEFF Research Database (Denmark)

    Täljsten, Björn; Arifovic, Fedja

    2009-01-01

    When RC beams are strengthened by FRP plates bonded to the bottom face of the beam they fail due to the lack of the mechanical anchorage of the plate. This paper shows that it is possible to relate the anchorage failure of tension face FRP-plated beams to the crack sliding capacity of the beams...... load of tension face plated beams. The assumption is that the FRP-plate must be extended at least one critical anchorage length beyond the theoretical occurrence of the critical diagonal crack....

  2. A novel bipartite nuclear localization signal guides BPM1 protein to nucleolus suggesting its Cullin3 independent function.

    Directory of Open Access Journals (Sweden)

    Dunja Leljak Levanić

    Full Text Available BPM1 belongs to the MATH-BTB family of proteins, which act as substrate-binding adaptors for the Cullin3-based E3 ubiquitin ligase. MATH-BTB proteins associate with Cullin3 via the BTB domain and with the substrate protein via the MATH domain. Few BPM1-interacting proteins with different functions are recognized, however, specific roles of BPM1, depending on its cellular localization have not been studied so far. Here, we found a novel bipartite nuclear localization signal at the C-terminus of the BPM1 protein, responsible for its nuclear and nucleolar localization and sufficient to drive the green fluorescent protein and cytoplasmic BPM4 protein into the nucleus. Co-localization analysis in live Nicotiana tabacum BY2 cells indicates a Cullin3 independent function since BPM1 localization is predominantly nucleolar and thus devoid of Cullin3. Treatment of BY2 cells with the proteasome inhibitor MG132 blocks BPM1 and Cullin3 degradation, suggesting turnover of both proteins through the ubiquitin-proteasome pathway. Possible roles of BPM1 in relation to its in vivo localization are discussed.

  3. Partial volume correction of the microPET blood input function using ensemble learning independent component analysis

    International Nuclear Information System (INIS)

    Medical images usually suffer from a partial volume effect (PVE), which may degrade the accuracy of any quantitative information extracted from the images. Our aim was to recreate accurate radioactivity concentration and time-activity curves (TACs) by microPET R4 quantification using ensemble learning independent component analysis (EL-ICA). We designed a digital cardiac phantom for this simulation and in order to evaluate the ability of EL-ICA to correct the PVE, the simulated images were convoluted using a Gaussian function (FWHM = 1-4 mm). The robustness of the proposed method towards noise was investigated by adding statistical noise (SNR = 2-16). During further evaluation, another set of cardiac phantoms were generated from the reconstructed images, and Poisson noise at different levels was added to the sinogram. In real experiments, four rat microPET images and a number of arterial blood samples were obtained; these were used to estimate the metabolic rate of FDG (MRFDG). Input functions estimated using the FastICA method were used for comparison. The results showed that EL-ICA could correct PVE in both the simulated and real cases. After correcting for the PVE, the errors for MRFDG, when estimated by the EL-ICA method, were smaller than those when TACs were directly derived from the PET images and when the FastICA approach was used.

  4. Willingness to Pay for Quality of Life Technologies to Enhance Independent Functioning Among Baby Boomers and the Elderly Adults

    Science.gov (United States)

    Schulz, Richard; Beach, Scott R.; Matthews, Judith T.; Courtney, Karen; Devito Dabbs, Annette; Person Mecca, Laurel; Sankey, Steadman Scott

    2014-01-01

    Purpose: We report the results of a study designed to assess whether and how much potential individual end users are willing to pay for Quality of Life Technologies (QoLTs) designed to enhance functioning and independence. Design and Methods: We carried out a web survey of a nationally representative sample of U.S. baby boomers (aged 45–64; N = 416) and older adults (aged 65 and greater, N = 114). Respondents were first instructed to assume that they needed help with kitchen activities/personal care and that technology was available to help with things like meal preparation/dressing, and then they were asked the most they would be willing to pay each month out of pocket for these technologies. Results: We modeled willingness to pay some (72% of respondents) versus none (28%), and the most people were willing to pay. Those willing to pay something were on average willing to pay a maximum of $40.30 and $45.00 per month for kitchen and personal care technology assistance, respectively. Respondents concerned about privacy or who were currently using assistive technology were less willing to pay. Respondents with higher incomes, who were Hispanic, or who perceived a higher likelihood of needing help in the future were more willing to pay. Implications: Consumers’ willingness to pay out of pocket for technologies to improve their well-being and independence is limited. In order to be widely adopted, QoLTs will have to be highly cost effective so that third party payers such as Medicare and private insurance companies are willing to pay for them. PMID:23528289

  5. Human Enterovirus Nonstructural Protein 2CATPase Functions as Both an RNA Helicase and ATP-Independent RNA Chaperone.

    Directory of Open Access Journals (Sweden)

    Hongjie Xia

    2015-07-01

    Full Text Available RNA helicases and chaperones are the two major classes of RNA remodeling proteins, which function to remodel RNA structures and/or RNA-protein interactions, and are required for all aspects of RNA metabolism. Although some virus-encoded RNA helicases/chaperones have been predicted or identified, their RNA remodeling activities in vitro and functions in the viral life cycle remain largely elusive. Enteroviruses are a large group of positive-stranded RNA viruses in the Picornaviridae family, which includes numerous important human pathogens. Herein, we report that the nonstructural protein 2CATPase of enterovirus 71 (EV71, which is the major causative pathogen of hand-foot-and-mouth disease and has been regarded as the most important neurotropic enterovirus after poliovirus eradication, functions not only as an RNA helicase that 3'-to-5' unwinds RNA helices in an adenosine triphosphate (ATP-dependent manner, but also as an RNA chaperone that destabilizes helices bidirectionally and facilitates strand annealing and complex RNA structure formation independently of ATP. We also determined that the helicase activity is based on the EV71 2CATPase middle domain, whereas the C-terminus is indispensable for its RNA chaperoning activity. By promoting RNA template recycling, 2CATPase facilitated EV71 RNA synthesis in vitro; when 2CATPase helicase activity was impaired, EV71 RNA replication and virion production were mostly abolished in cells, indicating that 2CATPase-mediated RNA remodeling plays a critical role in the enteroviral life cycle. Furthermore, the RNA helicase and chaperoning activities of 2CATPase are also conserved in coxsackie A virus 16 (CAV16, another important enterovirus. Altogether, our findings are the first to demonstrate the RNA helicase and chaperoning activities associated with enterovirus 2CATPase, and our study provides both in vitro and cellular evidence for their potential roles during viral RNA replication. These findings

  6. Urban contaminants project: Data for sediment, fish and eggs collected from Chester Creek, Anchorage, Alaska 1991

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — Urbanization has degraded the water quality and adversely impacted biological communities in the lakes and streams of Anchorage. Pollution from point and nonpoint...

  7. CFRP筋复合型锚具锚固性能研究%Research on Anchorage Performance of Wedge-bond Anchorage for CFRP Tendons

    Institute of Scientific and Technical Information of China (English)

    刘荣桂; 刘德鑫; 延永东

    2013-01-01

    In order to solve the anchorage problems for carbon fiber reinforced polymer (CFRP) tendons,a wedge-bond anchorage for CFRP tendons was developed.This anchorage consisted of bonding material,metal sleeve,clip and conical barrel.Influence factors of anchorage performance,such as length of clip,length of metal sleeve,clip clamping position and bonding material were illustrated.Results show that bonding material,length of metal sleeve,and clip clamping position will have significant influence on anchorage performance.The clip clamping position should be avoided at both ends of the metal sleeve,and should make compression zone metal sleeve length be less than that of the tension zone length.Meanwhile,the anchorage performance of bonding material Lica-B is better than that of bonding material Lica-A.The each component of wedge-bond anchorage has good cooperative work performance,and there is no relative slip between clip and metal sleeve.The wedge-bond anchorage with reasonable parameters can anchor CFRP tendons with diameter of 8 mm effectively.%为解决碳纤维增强复合材料(CFRP)筋的锚固问题,研制了由粘结材料、金属筒、夹片和锚环组成的CFRP筋复合型锚具,并进行了夹片长度、金属筒长度、夹片夹持位置以及粘结材料对CFRP筋复合型锚具的锚固性能影响试验.结果表明:粘结材料、金属筒长度和夹片夹持位置均对锚固性能有较大影响;夹片的夹持位置应避免在金属筒两端,且宜使金属筒受压区长度小于受拉区长度;Lica-B型粘结材料锚固性能优于Lica-A型粘结材料;复合型锚具各组件协同工作性能良好,夹片与金属筒间无滑移产生;参数合理的该类复合型锚具可有效锚固直径为8 mm的CFRP筋.

  8. SmD1 Modulates the miRNA Pathway Independently of Its Pre-mRNA Splicing Function.

    Directory of Open Access Journals (Sweden)

    Xiao-Peng Xiong

    2015-08-01

    Full Text Available microRNAs (miRNAs are a class of endogenous regulatory RNAs that play a key role in myriad biological processes. Upon transcription, primary miRNA transcripts are sequentially processed by Drosha and Dicer ribonucleases into ~22-24 nt miRNAs. Subsequently, miRNAs are incorporated into the RNA-induced silencing complexes (RISCs that contain Argonaute (AGO family proteins and guide RISC to target RNAs via complementary base pairing, leading to post-transcriptional gene silencing by a combination of translation inhibition and mRNA destabilization. Select pre-mRNA splicing factors have been implicated in small RNA-mediated gene silencing pathways in fission yeast, worms, flies and mammals, but the underlying molecular mechanisms are not well understood. Here, we show that SmD1, a core component of the Drosophila small nuclear ribonucleoprotein particle (snRNP implicated in splicing, is required for miRNA biogenesis and function. SmD1 interacts with both the microprocessor component Pasha and pri-miRNAs, and is indispensable for optimal miRNA biogenesis. Depletion of SmD1 impairs the assembly and function of the miRISC without significantly affecting the expression of major canonical miRNA pathway components. Moreover, SmD1 physically and functionally associates with components of the miRISC, including AGO1 and GW182. Notably, miRNA defects resulting from SmD1 silencing can be uncoupled from defects in pre-mRNA splicing, and the miRNA and splicing machineries are physically and functionally distinct entities. Finally, photoactivatable-ribonucleoside-enhanced crosslinking and immunoprecipitation (PAR-CLIP analysis identifies numerous SmD1-binding events across the transcriptome and reveals direct SmD1-miRNA interactions. Our study suggests that SmD1 plays a direct role in miRNA-mediated gene silencing independently of its pre-mRNA splicing activity and indicates that the dual roles of splicing factors in post-transcriptional gene regulation may be

  9. Mechanical properties and failure characteristics of fractured sandstone with grouting and anchorage

    Institute of Scientific and Technical Information of China (English)

    Zong Yijiang; Han Lijun; Qu Tao; Yang Shengqi

    2014-01-01

    Based on uniaxial compression experimental results on fractured sandstone with grouting and anchorage, we studied the strength and deformation properties, the failure model, crack formation and evolution laws of fractured sandstone under different conditions of anchorage. The experimental results show that the strength and elastic modulus of fractured sandstone with different fracture angles are sig-nificantly lower than those of intact sandstone. Compared with the fractured samples without anchorage, the peak strength, residual strength, peak and ultimate axial strain of fractured sandstone under different anchorage increase by 64.5-320.0%, 62.8-493.0%, and 31.6-181.4%, respectively. The number of bolts and degree of pre-stress has certain effects on the peak strength and failure model of fractured sandstone. The peak strength of fractured sandstone under different anchorage increases to some extent, and the failure model of fractured sandstone also transforms from tensile failure to tensile-shear mixed failure with the number of bolts. The pre-stress can restrain the formation and evolution process of tensile cracks, delay the failure process of fractured sandstone under anchorage and impel the transformation of failure model from brittle failure to plastic failure.

  10. Functional Diversity of Human Mitochondrial J-proteins Is Independent of Their Association with the Inner Membrane Presequence Translocase.

    Science.gov (United States)

    Sinha, Devanjan; Srivastava, Shubhi; D'Silva, Patrick

    2016-08-12

    Mitochondrial J-proteins play a critical role in governing Hsp70 activity and, hence, are essential for organellar protein translocation and folding. In contrast to yeast, which has a single J-protein Pam18, humans involve two J-proteins, DnaJC15 and DnaJC19, associated with contrasting cellular phenotype, to transport proteins into the mitochondria. Mutation in DnaJC19 results in dilated cardiomyopathy and ataxia syndrome, whereas expression of DnaJC15 regulates the response of cancer cells to chemotherapy. In the present study we have comparatively assessed the biochemical properties of the J-protein paralogs in relation to their association with the import channel. Both DnaJC15 and DnaJC19 formed two distinct subcomplexes with Magmas at the import channel. Knockdown analysis suggested an essential role for Magmas and DnaJC19 in organellar protein translocation and mitochondria biogenesis, whereas DnaJC15 had dispensable supportive function. The J-proteins were found to have equal affinity for Magmas and could stimulate mitochondrial Hsp70 ATPase activity by equivalent levels. Interestingly, we observed that DnaJC15 exhibits bifunctional properties. At the translocation channel, it involves conserved interactions and mechanism to translocate the precursors into mitochondria. In addition to protein transport, DnaJC15 also showed a dual role in yeast where its expression elicited enhanced sensitivity of cells to cisplatin that required the presence of a functional J-domain. The amount of DnaJC15 expressed in the cell was directly proportional to the sensitivity of cells. Our analysis indicates that the differential cellular phenotype displayed by human mitochondrial J-proteins is independent of their activity and association with Magmas at the translocation channel.

  11. EPO-independent functional EPO receptor in breast cancer enhances estrogen receptor activity and promotes cell proliferation

    Energy Technology Data Exchange (ETDEWEB)

    Reinbothe, Susann; Larsson, Anna-Maria; Vaapil, Marica; Wigerup, Caroline [Department of Laboratory Medicine, Translational Cancer Research, Medicon Village, Lund University, SE-223 81 Lund (Sweden); CREATE Health, Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv (Israel); Sun, Jianmin [Department of Laboratory Medicine, Translational Cancer Research, Medicon Village, Lund University, SE-223 81 Lund (Sweden); Jögi, Annika [Department of Laboratory Medicine, Translational Cancer Research, Medicon Village, Lund University, SE-223 81 Lund (Sweden); CREATE Health, Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv (Israel); Neumann, Drorit [Department of Cell and Developmental Biology, Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv (Israel); Rönnstrand, Lars [Department of Laboratory Medicine, Translational Cancer Research, Medicon Village, Lund University, SE-223 81 Lund (Sweden); Påhlman, Sven, E-mail: sven.pahlman@med.lu.se [Department of Laboratory Medicine, Translational Cancer Research, Medicon Village, Lund University, SE-223 81 Lund (Sweden); CREATE Health, Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv (Israel)

    2014-02-28

    Highlights: • New anti-human EPOR antibody confirms full-length EPOR expression in breast cancer cells. • Proliferation of breast cancer cells is not affected by rhEPO treatment in vitro. • EPOR knockdown impairs proliferation of ERa positive breast cancer cells. • EPOR knockdown reduces AKT phosphorylation and ERa activity. - Abstract: The main function of Erythropoietin (EPO) and its receptor (EPOR) is the stimulation of erythropoiesis. Recombinant human EPO (rhEPO) is therefore used to treat anemia in cancer patients. However, clinical trials have indicated that rhEPO treatment might promote tumor progression and has a negative effect on patient survival. In addition, EPOR expression has been detected in several cancer forms. Using a newly produced anti-EPOR antibody that reliably detects the full-length isoform of the EPOR we show that breast cancer tissue and cells express the EPOR protein. rhEPO stimulation of cultured EPOR expressing breast cancer cells did not result in increased proliferation, overt activation of EPOR (receptor phosphorylation) or a consistent activation of canonical EPOR signaling pathway mediators such as JAK2, STAT3, STAT5, or AKT. However, EPOR knockdown experiments suggested functional EPO receptors in estrogen receptor positive (ERα{sup +}) breast cancer cells, as reduced EPOR expression resulted in decreased proliferation. This effect on proliferation was not seen in ERα negative cells. EPOR knockdown decreased ERα activity further supports a mechanism by which EPOR affects proliferation via ERα-mediated mechanisms. We show that EPOR protein is expressed in breast cancer cells, where it appears to promote proliferation by an EPO-independent mechanism in ERα expressing breast cancer cells.

  12. Functional Diversity of Human Mitochondrial J-proteins Is Independent of Their Association with the Inner Membrane Presequence Translocase.

    Science.gov (United States)

    Sinha, Devanjan; Srivastava, Shubhi; D'Silva, Patrick

    2016-08-12

    Mitochondrial J-proteins play a critical role in governing Hsp70 activity and, hence, are essential for organellar protein translocation and folding. In contrast to yeast, which has a single J-protein Pam18, humans involve two J-proteins, DnaJC15 and DnaJC19, associated with contrasting cellular phenotype, to transport proteins into the mitochondria. Mutation in DnaJC19 results in dilated cardiomyopathy and ataxia syndrome, whereas expression of DnaJC15 regulates the response of cancer cells to chemotherapy. In the present study we have comparatively assessed the biochemical properties of the J-protein paralogs in relation to their association with the import channel. Both DnaJC15 and DnaJC19 formed two distinct subcomplexes with Magmas at the import channel. Knockdown analysis suggested an essential role for Magmas and DnaJC19 in organellar protein translocation and mitochondria biogenesis, whereas DnaJC15 had dispensable supportive function. The J-proteins were found to have equal affinity for Magmas and could stimulate mitochondrial Hsp70 ATPase activity by equivalent levels. Interestingly, we observed that DnaJC15 exhibits bifunctional properties. At the translocation channel, it involves conserved interactions and mechanism to translocate the precursors into mitochondria. In addition to protein transport, DnaJC15 also showed a dual role in yeast where its expression elicited enhanced sensitivity of cells to cisplatin that required the presence of a functional J-domain. The amount of DnaJC15 expressed in the cell was directly proportional to the sensitivity of cells. Our analysis indicates that the differential cellular phenotype displayed by human mitochondrial J-proteins is independent of their activity and association with Magmas at the translocation channel. PMID:27330077

  13. EPO-independent functional EPO receptor in breast cancer enhances estrogen receptor activity and promotes cell proliferation

    International Nuclear Information System (INIS)

    Highlights: • New anti-human EPOR antibody confirms full-length EPOR expression in breast cancer cells. • Proliferation of breast cancer cells is not affected by rhEPO treatment in vitro. • EPOR knockdown impairs proliferation of ERa positive breast cancer cells. • EPOR knockdown reduces AKT phosphorylation and ERa activity. - Abstract: The main function of Erythropoietin (EPO) and its receptor (EPOR) is the stimulation of erythropoiesis. Recombinant human EPO (rhEPO) is therefore used to treat anemia in cancer patients. However, clinical trials have indicated that rhEPO treatment might promote tumor progression and has a negative effect on patient survival. In addition, EPOR expression has been detected in several cancer forms. Using a newly produced anti-EPOR antibody that reliably detects the full-length isoform of the EPOR we show that breast cancer tissue and cells express the EPOR protein. rhEPO stimulation of cultured EPOR expressing breast cancer cells did not result in increased proliferation, overt activation of EPOR (receptor phosphorylation) or a consistent activation of canonical EPOR signaling pathway mediators such as JAK2, STAT3, STAT5, or AKT. However, EPOR knockdown experiments suggested functional EPO receptors in estrogen receptor positive (ERα+) breast cancer cells, as reduced EPOR expression resulted in decreased proliferation. This effect on proliferation was not seen in ERα negative cells. EPOR knockdown decreased ERα activity further supports a mechanism by which EPOR affects proliferation via ERα-mediated mechanisms. We show that EPOR protein is expressed in breast cancer cells, where it appears to promote proliferation by an EPO-independent mechanism in ERα expressing breast cancer cells

  14. Does a Wii-based exercise program enhance balance control of independently functioning older adults? A systematic review

    Science.gov (United States)

    Laufer, Yocheved; Dar, Gali; Kodesh, Einat

    2014-01-01

    Background Exercise programs that challenge an individual’s balance have been shown to reduce the risk of falls among older adults. Virtual reality computer-based technology that provides the user with opportunities to interact with virtual objects is used extensively for entertainment. There is a growing interest in the potential of virtual reality-based interventions for balance training in older adults. This work comprises a systematic review of the literature to determine the effects of intervention programs utilizing the Nintendo Wii console on balance control and functional performance in independently functioning older adults. Methods Studies were obtained by searching the following databases: PubMed, CINAHL, PEDro, EMBASE, SPORTdiscus, and Google Scholar, followed by a hand search of bibliographic references of the included studies. Included were randomized controlled trials written in English in which Nintendo Wii Fit was used to enhance standing balance performance in older adults and compared with an alternative exercise treatment, placebo, or no treatment. Results Seven relevant studies were retrieved. The four studies examining the effect of Wii-based exercise compared with no exercise reported positive effects on at least one outcome measure related to balance performance in older adults. Studies comparing Wii-based training with alternative exercise programs generally indicated that the balance improvements achieved by Wii-based training are comparable with those achieved by other exercise programs. Conclusion The review indicates that Wii-based exercise programs may serve as an alternative to more conventional forms of exercise aimed at improving balance control. However, due to the great variability between studies in terms of the intervention protocols and outcome measures, as well as methodological limitations, definitive recommendations as to optimal treatment protocols and the potential of such an intervention as a safe and effective home

  15. Bst1 is required for Candida albicans infecting host via facilitating cell wall anchorage of Glycosylphosphatidyl inositol anchored proteins

    Science.gov (United States)

    Liu, Wei; Zou, Zui; Huang, Xin; Shen, Hui; He, Li Juan; Chen, Si Min; Li, Li Ping; Yan, Lan; Zhang, Shi Qun; Zhang, Jun Dong; Xu, Zheng; Xu, Guo Tong; An, Mao Mao; Jiang, Yuan Ying

    2016-01-01

    Glycosylphosphatidyl inositol anchored proteins (GPI-APs) on fungal cell wall are essential for invasive infections. While the function of inositol deacylation of GPI-APs in mammalian cells has been previously characterized the impact of inositol deacylation in fungi and implications to host infection remains largely unexplored. Herein we describe our identification of BST1, an inositol deacylase of GPI-Aps in Candida albicans, was critical for GPI-APs cell wall attachment and host infection. BST1-deficient C. albicans (bst1Δ/Δ) was associated with severely impaired cell wall anchorage of GPI-APs and subsequen unmasked β-(1,3)-glucan. Consistent with the aberrant cell wall structures, bst1Δ/Δ strain did not display an invasive ability and could be recognized more efficiently by host immune systems. Moreover, BST1 null mutants or those expressing Bst1 variants did not display inositol deacylation activity and exhibited severely attenuated virulence and reduced organic colonization in a murine systemic candidiasis model. Thus, Bst1 can facilitate cell wall anchorage of GPI-APs in C. albicans by inositol deacylation, and is critical for host invasion and immune escape. PMID:27708385

  16. Transfer and anchorage bond behaviour in self-compacting concrete

    Directory of Open Access Journals (Sweden)

    Rigueira-Víctor, J. W.

    2006-12-01

    Full Text Available Self-compacting concretes (SCC provide solutions to the problems facing precast concrete construction, enhancing competitiveness, reducing turnaround times and improving final product quality. SCC is fast becoming a key product for the future development of the precast pre-stressed concrete industry.The present paper compares the bond performance of SCC and traditional concrete (TC. The bond performance results confirm the viability of SCC in precast pre-stressed concrete manufacture, despite a slightly higher loss of pre-stressing force and slightly greater anchorage lengths in SCC with a low water/cement ratio. No differences in transfer or anchorage length were detected,however, when high strength TC and SCC were compared. The ECADA test method proved to be well suited to detecting the differences between the concretes analyzed.El desarrollo de los hormigones autocompactantes (SCCofrece muchas posibilidades a las construcciones con hormigón prefabricado, aumentando su competitividad, reduciéndolos plazos de fabricación y ofreciendo mejoras en la calidad del producto final. El SCC se está convirtiendo en un producto clave para el futuro desarrollo de la industria de prefabricados de hormigón pretensado.En este estudio se compara el comportamiento adherente de los SCC con el de los hormigones tradicionales (TC actuales. Los resultados obtenidos confirman la viabilidad del uso de los SCC para la fabricación de elementos prefabricados con hormigón pretensado, en lo relativo a su comportamiento adherente, aunque con la necesidad de considerar unas pérdidas de pretensado ligeramente mayores. Asimismo,debe esperarse un ligero aumento de las longitudes de anclaje cuando se trabaje con SCC de baja relación agua/cemento. Sin embargo, no se han detectado diferencias de comportamiento entre ambos tipos de hormigón cuando la resistencia a compresión es alta en lo relativo a las longitudes de transmisión y anclaje. El método de ensayo ECADA

  17. Functional Independence Measure in Iran: A Confirmatory Factor Analysis and Evaluation of Ceiling and Floor Effects in Traumatic Brain Injury Patients

    OpenAIRE

    Rezaei; Dehnadi Moghadam; Khodadadi; Rahmatpour

    2015-01-01

    Background The functional independence measure (FIM) is one of the most important assessment instruments for motor and cognitive dependence in rehabilitation medicine; however, there is little data about its confirmatory factor analysis (CFA) and ceiling/floor effects from other countries and also in Iranian patients. Objectives The aim of this study was to evaluate a two-factor model (motor and cognitive independence as latent va...

  18. Independent component analysis using prior information for signal detection in a functional imaging system of the retina

    NARCIS (Netherlands)

    Barriga, E. Simon; Pattichis, Marios; Ts’o, Dan; Abramoff, Michael; Kardon, Randy; Kwon, Young; Soliz, Peter

    2011-01-01

    Independent component analysis (ICA) is a statistical technique that estimates a set of sources mixed by an unknown mixing matrix using only a set of observations. For this purpose, the only assumption is that the sources are statistically independent. In many applications, some information about th

  19. Virtual planning for vertical control using temporary anchorage devices

    Directory of Open Access Journals (Sweden)

    Mauricio Accorsi

    2015-01-01

    Full Text Available The new and innovative technologies are unprecedentedly improving the level of proficiency in orthodontics in the recent history of this area of expertise. The proliferation of advances, such as self-ligating systems, temporary anchorage devices, shape-memory wires, robotically wire bending, intraoral scanners, cone-beam computed tomography, bring the virtual planning, and confection of dental devices through CAD/CAM systems to the real world. In order to get efficiency and efficacy in orthodontics with these new technologies, we must understand the importance of systemically managed clinical information, medical, and dentistry history of the patients, including the images resources, which ensures the use of a communication that is assisted by the technology, with an interdisciplinary team so that the database is able to help and support the process of therapeutic decision-making. This paper presents the clinical case of a borderline patient for orthognathic surgery who had his final treatment planning supported by these new tools for three-dimensional diagnosis and virtual planning.

  20. Does a Wii-based exercise program enhance balance control of independently functioning older adults? A systematic review

    Directory of Open Access Journals (Sweden)

    Laufer Y

    2014-10-01

    Full Text Available Yocheved Laufer, Gali Dar, Einat Kodesh Physical Therapy Department, Faculty of Social Welfare and Health Sciences, University of Haifa, Haifa, Israel Background: Exercise programs that challenge an individual’s balance have been shown to reduce the risk of falls among older adults. Virtual reality computer-based technology that provides the user with opportunities to interact with virtual objects is used extensively for entertainment. There is a growing interest in the potential of virtual reality-based interventions for balance training in older adults. This work comprises a systematic review of the literature to determine the effects of intervention programs utilizing the Nintendo Wii console on balance control and functional performance in independently functioning older adults.Methods: Studies were obtained by searching the following databases: PubMed, CINAHL, PEDro, EMBASE, SPORTdiscus, and Google Scholar, followed by a hand search of bibliographic references of the included studies. Included were randomized controlled trials written in English in which Nintendo Wii Fit was used to enhance standing balance performance in older adults and compared with an alternative exercise treatment, placebo, or no treatment.Results: Seven relevant studies were retrieved. The four studies examining the effect of Wii-based exercise compared with no exercise reported positive effects on at least one outcome measure related to balance performance in older adults. Studies comparing Wii-based training with alternative exercise programs generally indicated that the balance improvements achieved by Wii-based training are comparable with those achieved by other exercise programs.Conclusion: The review indicates that Wii-based exercise programs may serve as an alternative to more conventional forms of exercise aimed at improving balance control. However, due to the great variability between studies in terms of the intervention protocols and outcome measures, as

  1. Guidelines for evaluation of anchorage adequacy for safety-related equipment typically used on WWER-type NPPs

    International Nuclear Information System (INIS)

    This report describes the criteria which should be met when the capacity evaluation of anchorage of safety related equipment is performed for the WWER type NPPs. It should be noted that these criteria were developed specifically for anchorage of WWER type equipment and components to the concrete or steel building structures and they cover different types of anchor bolts and other anchorage details which are typical just for the existing, constructed or reconstructed WWER type NPPs. The screening approach for verifying of equipment anchorage presented in this report is based on a combination of inspections, calculations, and engineering judgement

  2. Experiment on Anchorage Performance of Bond-type Anchorage for CFRP Tendon%CFRP筋粘结式锚具锚固性能试验

    Institute of Scientific and Technical Information of China (English)

    蒋田勇; 方志

    2011-01-01

    针对碳纤维增强塑料(CFRP)作为预应力筋或拉索时出现的锚固问题,开发了一种以活性粉末混凝土(RPC)作为粘结介质的粘结式锚具.建立了平均粘结强度及其对应的滑移量、临界锚固长度以及粘结滑移本构模型等计算公式.通过静载试验研究了筋材表面形状、锚固长度、根数、间距、套筒内壁倾角以及筋材预张拉等对锚固性能的影响.结果表明:CFRP筋的表面形状对以RPC作为粘结介质的粘结式锚具的锚固性能有着决定性影响;当表面压纹CFRP筋的抗拉强度不大于3 000 MPa,RPC抗压强度不小于130 MPa时,对于普通粘结试件,临界锚团长度为20倍筋材直径;对于预张拉粘结试件,当预张比为56%时,锚固系统具有最短的临界锚固长度为13倍筋材直径;多筋同时锚固时,其间距不宜小于1倍筋材直径.%Aimed at anchorage problems that carbon fiber reinforced polymer/plastics (CFRP)was used as prestress tendon and cable reactive powder concrete (RPC) was regarded as bond medium and based on it, a new bond-type anchorage was developed.The calculated formulas of average bond strength and its corresponding slip, critical anchorage length, and bond-slip constitutive model were proposed.Influences of surface shape, bond length, number and space of CFRP tendons, slope angle of inner wall of steel sleeve and pretensioning load on anchorage performance were studied by static test.Test results show that the surface shape of CFRP tendon is significant to the anchorage performance of the bond-type anchorage.When the tensile strength of the indented surface CFRP tendon is less than 3 000 MPa and compressive strength of RPC is more than 130 MPa, the critical anchorage length is 20 diameters of general CFRP tendon for common bond specimen, the shortest critical anchorage length in anchorage system is 13 diameters of pretensioning CFRP tendon with ratio of 56 % of pretensioning load.The appropriate rod space is

  3. Promoting physical activity for elders with compromised function: the Lifestyle Interventions and Independence for Elders (LIFE Study physical activity intervention

    Directory of Open Access Journals (Sweden)

    Rejeski WJ

    2013-09-01

    Full Text Available W Jack Rejeski,1 Robert Axtell,2 Roger Fielding,3 Jeffrey Katula,1 Abby C King,4 Todd M Manini,5 Anthony P Marsh,1 Marco Pahor,5 Alvito Rego,6 Catrine Tudor-Locke,7 Mark Newman,8 Michael P Walkup,9 Michael E Miller9  On behalf of the LIFE Study Investigator Group 1Department of Health and Exercise Science, Wake Forest University, Winston-Salem, NC, 2Exercise Science Department, Southern Connecticut State University, New Haven, CT, 3Nutrtion, Exercise Physiology, and Sarcopenia Laboratory, Jean Mayer US Department of Agriculture Human Nutrition Research Center on Aging at Tufts University, Boston, MA, 4Department of Health Research and Policy and Stanford Prevention Research Center, Department of Medicine, Stanford University School of Medicine, Palo Alto, CA, 5Department of Aging and Geriatric Research, College of Medicine, University of Florida, Gainesville, FL, 6Department of Internal Medicine, Northwestern School of Medicine, Chicago, IL, 7Pennington Biomedical Research Center, Baton Rouge, LA, 8Department of Epidemiology, University of Pittsburgh, Pittsburgh, PA, 9Department of Biostatistical Sciences, Division of Public Health Sciences, School of Medicine, Wake Forest University, Winston-Salem, NC, USA Abstract: The Lifestyle Interventions and Independence for Elders (LIFE Study is a Phase III randomized controlled clinical trial (Clinicaltrials.gov identifier: NCT01072500 that will provide definitive evidence regarding the effect of physical activity (PA on major mobility disability in older adults (70–89 years old who have compromised physical function. This paper describes the methods employed in the delivery of the LIFE Study PA intervention, providing insight into how we promoted adherence and monitored the fidelity of treatment. Data are presented on participants' motives and self-perceptions at the onset of the trial along with accelerometry data on patterns of PA during exercise training. Prior to the onset of training, 31.4% of

  4. Bolt Pull-Out Tests of Anchorage Body under Different Loading Rates

    Directory of Open Access Journals (Sweden)

    Tong-bin Zhao

    2015-01-01

    Full Text Available Based on the force analysis and mechanical transmission mechanism of grouting bolts, the self-developed test apparatus for interfacial mechanics is used to study the distribution rule of axial force and interfacial stress of bolts in anchorage body. At the same time, pull-out tests of anchorage body are simulated with the particle flow code software PFC2D, and stress distribution and failure patters are researched under different loading rates. The results show that the distribution of axial force and interfacial shear stress is nonuniform along the anchorage section: axial force decreases, shear force increases first and then decreases, and the maximum value of both of them is closed to the pull-out side; with the increase of loading rates, both of axial force and interfacial shear stress show a trend of increase in the upper anchorage section but changes are not obvious in the lower anchorage section, which causes serious stress concentration; failure strength of pull-out and loading rates show a linear correlation; according to loading rates’ impact on the anchoring effect, the loading rates’ scope can be divided into soft scope (v100 mm/s.

  5. The Influence of CFRP Anchorage on Achieving Sectional Flexural Capacity of Strengthened Concrete Beams

    Directory of Open Access Journals (Sweden)

    Hayder A. Rasheed

    2015-12-01

    Full Text Available This research program is intended to verify the influence of using distributed external U-wrap CFRP anchorage to shift the failure mode from overall debonding to sectional flexural failure for concrete beams externally bonded with CFRP sheets. Premature cover delamination and FRP debonding are predominant failure modes in FRP flexural strengthening that may be delayed or prevented by using FRP anchorage. The present experimental study aims to comparatively prove that proper anchorage of flexural strengthening is anticipated to yield a classical flexural failure by FRP rupture or concrete crushing. Once the cohesion of concrete and/or the adhesion with the FRP is exhausted, the U-wraps are engaged to provide anchorage to the flexural FRP through shear friction. Accordingly, three identical T beams and three identical rectangular beams were designed and constructed to examine the capacity improvement by preventing premature debonding failure. The first specimen in each series was tested as a control beam. The second specimen in each series was strengthened using five layers of flexural CFRP in order to admit a debonding failure. The third specimen in each series was strengthened with the same five layers of flexural CFRP plus additional transverse CFRP U-wraps. This study proved that it is possible to quantify the higher flexural capacity of CFRP strengthened beams using external anchorage.

  6. Experimental study and stress analysis of rock bolt anchorage performance

    Directory of Open Access Journals (Sweden)

    Yu Chen

    2014-10-01

    Full Text Available A new method was developed to apply pull-and-shear loads to the bolt specimen in order to evaluate the anchorage performance of the rebar bolt and the D-Bolt. In the tests, five displacing angles (0°, 20°, 40°, 60°, and 90°, two joint gaps (0 mm and 30 mm, and three kinds of host rock materials (weak concrete, strong concrete, and concrete-granite were considered, and stress–strain measurements were conducted. Results show that the ultimate loads of both the D-Bolt and the rebar bolt remained constant with any displacing angles. The ultimate displacement of the D-Bolt changed from 140 mm at the 0° displacing angle (pure pull to approximately 70 mm at a displacing angle greater than 40°. The displacement capacity of the D-Bolt is approximately 3.5 times that of the rebar bolt under pure pull and 50% higher than that of the rebar bolt under pure shear. The compressive stress exists at 50 mm from the bolt head, and the maximum bending moment value rises with the increasing displacing angle. The rebar bolt mobilises greater applied load than the D-Bolt when subjected to the maximum bending. The yielding length (at 0° of the D-Bolt is longer than that of the rebar bolt. The displacement capacity of the bolts increased with the joint gap. The bolt subjected to joint gap effect yields more quickly with greater bending moment and smaller applied load. The displacement capacities of the D-Bolt and the rebar bolt are greater in the weak host rock than that in the hard host rock. In pure shear condition, the ultimate load of the bolts slightly decreases in the hard rock. The yielding speed in the hard rock is higher than that in the weak rock.

  7. Experimental study and stress analysis of rock bolt anchorage performance

    Institute of Scientific and Technical Information of China (English)

    Yu Chen

    2014-01-01

    A new method was developed to apply pull-and-shear loads to the bolt specimen in order to evaluate the anchorage performance of the rebar bolt and the D-Bolt. In the tests, five displacing angles (0?, 20?, 40?, 60?, and 90?), two joint gaps (0 mm and 30 mm), and three kinds of host rock materials (weak concrete, strong concrete, and concrete-granite) were considered, and stressestrain measurements were con-ducted. Results show that the ultimate loads of both the D-Bolt and the rebar bolt remained constant with any displacing angles. The ultimate displacement of the D-Bolt changed from 140 mm at the 0? displacing angle (pure pull) to approximately 70 mm at a displacing angle greater than 40?. The displacement capacity of the D-Bolt is approximately 3.5 times that of the rebar bolt under pure pull and 50%higher than that of the rebar bolt under pure shear. The compressive stress exists at 50 mm from the bolt head, and the maximum bending moment value rises with the increasing displacing angle. The rebar bolt mobilises greater applied load than the D-Bolt when subjected to the maximum bending. The yielding length (at 0?) of the D-Bolt is longer than that of the rebar bolt. The displacement capacity of the bolts increased with the joint gap. The bolt subjected to joint gap effect yields more quickly with greater bending moment and smaller applied load. The displacement capacities of the D-Bolt and the rebar bolt are greater in the weak host rock than that in the hard host rock. In pure shear condition, the ultimate load of the bolts slightly decreases in the hard rock. The yielding speed in the hard rock is higher than that in the weak rock.

  8. Pursuing Perfection: Distress and Interpersonal Functioning among Adolescent Boys in Single-Sex and Co-Educational Independent Schools

    Science.gov (United States)

    Coren, Sidney A.; Luthar, Suniya S.

    2014-01-01

    This study extends past findings of heightened problems among affluent youth by examining adjustment patterns among boys in two academically elite, independent high schools: one for boys only and the other co-educational. Both samples manifested disproportionately high rates of internalizing and externalizing symptoms, but only the co-educational…

  9. Ground Water in the Anchorage Area, Alaska--Meeting the Challenges of Ground-Water Sustainability

    Science.gov (United States)

    Moran, Edward H.; Galloway, Devin L.

    2006-01-01

    Ground water is an important component of Anchorage's water supply. During the 1970s and early 80s when ground water extracted from aquifers near Ship Creek was the principal source of supply, area-wide declines in ground-water levels resulted in near record low streamflows in Ship Creek. Since the importation of Eklutna Lake water in the late 1980s, ground-water use has been reduced and ground water has contributed 14-30 percent of the annual supply. As Anchorage grows, given the current constraints on the Eklutna Lake water availability, the increasing demand for water could place an increasing reliance on local ground-water resources. The sustainability of Anchorage's ground-water resources challenges stakeholders to develop a comprehensive water-resources management strategy.

  10. Numerical Simulation and Experimental Validation of an Integrated Sleeve-Wedge Anchorage for CFRP Rods

    DEFF Research Database (Denmark)

    Schmidt, Jacob Wittrup; Smith, Scott T.; Täljsten, Björn;

    2011-01-01

    The tensioning of carbon fibre-reinforced polymer (CFRP) rods for prestressed concrete applications or post-tensioning repair and strengthening has met with mixed success. This is primarily due to limitations inherent in the use of traditional wedge anchors typically used for steel tendons....... Recently, an integrated sleeve-wedge anchorage has been successfully developed specifically for CFRP rods. This paper in turn presents a numerical simulation of the newly developed anchorage using ABAQUS. The three-dimensional finite element (FE) model, which considers material non-linearity, uses...... hexagonal elements for the barrel and CFRP rod and tetrahedral elements for the integrated sleeve-wedge. The simulated barrel surface strains are shown to compare well with optically measured strains, however, the numerical results are shown to be sensitive to the mechanical properties of the anchorage...

  11. Independent Directors

    DEFF Research Database (Denmark)

    Ringe, Wolf-Georg

    2013-01-01

    This paper re-evaluates the corporate governance concept of ‘board independence’ against the disappointing experiences during the 2007-08 financial crisis. Independent or outside directors had long been seen as an essential tool to improve the monitoring role of the board. Yet the crisis revealed...... independence. It would redefine independence to include those directors that are independent of the firm's controller, but, at the same time, it would require them to be more accountable to (minority) shareholders....

  12. DIRECT USAGE OF MINISCREW ANCHORAGE TO INTRUDE OVERERUPTED MAXILLARY POSTERIOR TEETH BEFORE PROSTHODONTIC PREPARATION:A CASE REPORT

    Directory of Open Access Journals (Sweden)

    Yasemin Bahar Acar

    2016-01-01

    Full Text Available Overeruption of maxillary molars due loss of opposing teeth creates occlusal and functional interferences. Before reconstruction can be initiated, intrusion of overerupted molars becomes essential. This report illustrates treatment of overerupted maxillary premolar and molar via direct use of miniscrew anchorage. A 24-year old female had lost first and second left mandibular molars due to pulpal necrotizing agents, resulting with a large alveolar bone defect and overerupted maxillary premolar and molar. She had a history of unsuccessful alveolar distraction of mandibular left premolars to increase the alveolar bone height prior to implant placement. Patient was satisfied with her smile and refused comprehensive orthodontic treatment. Maxillary premolar and molar were intruded segmentally for 4mm in 8 months, using a combination of a mini-implant and partialfixed edgewise appliances. Biological responses of teeth and surrounding bony structures to intrusion appeared normal and acceptable in radiographic and clinical examination.

  13. A Study of the system control model of caisson dewatering of the north anchorage of Taizhou Bridge

    Institute of Scientific and Technical Information of China (English)

    Zhao Yanrong; Zhou Zhifang; Ruan Jing

    2011-01-01

    A caisson foundation is applied to the north anchorage of Taizhou Yangtze River Highway Bridge of which the initial caisson sinking requires dewatering. Since the caisson foundation is quite close to nearby buildings, a system control model is established with source (sink) distribution and intensity being the object function, minimum requirements of settlement and deformation of surroundings caused by dewatering and dynamic water levels during different working procedures being constraints, and the design parameter of pumping wells being the variable, so as to lower the jeopardizing of surrounding buildings, which provides a new method for active control over settlement during dewatering. Such a method of dewatering based on system control model should be of significance for similar projects involving dewatering.

  14. Small molecule screening reveals a transcription-independent pro-survival function of androgen receptor in castration-resistant prostate cancer.

    Science.gov (United States)

    Narizhneva, Natalia V; Tararova, Natalia D; Ryabokon, Petro; Shyshynova, Inna; Prokvolit, Anatoly; Komarov, Pavel G; Purmal, Andrei A; Gudkov, Andrei V; Gurova, Katerina V

    2009-12-15

    In prostate cancer (PCa) patients, initial responsiveness to androgen deprivation therapy is frequently followed by relapse due to development of treatment-resistant androgen-independent PCa. This is typically associated with acquisition of mutations in AR that allow activity as a transcription factor in the absence of ligand, indicating that androgen-independent PCa remains dependent on AR function. Our strategy to effectively target AR in androgen-independent PCa involved using a cell-based readout to isolate small molecules that inhibit AR transactivation function through mechanisms other than modulation of ligand binding. A number of the identified inhibitors were toxic to AR-expressing PCa cells regardless of their androgen dependence. Among these, some only suppressed PCa cell growth (ARTIS), while others induced cell death (ARTIK). ARTIK, but not ARTIS, compounds caused disappearance of AR protein from treated cells. siRNA against AR behaved like ARTIK compounds, while a dominant negative AR mutant that prevents AR-mediated transactivation but does not eliminate the protein showed only a growth suppressive effect. These observations reveal a transcription-independent function of AR that is essential for PCa cell viability and, therefore, is an ideal target for anti-PCa treatment. Indeed, several of the identified AR inhibitors demonstrated in vivo efficacy in mouse models of PCa and are candidates for pharmacologic optimization.

  15. Using the Deutsch-Jozsa algorithm to determine parts of an array and apply a specified function to each independent part

    CERN Document Server

    Lipovaca, Samir

    2009-01-01

    Using the Deutsch-Jozsa algorithm, we will develop a method for solving a class of problems in which we need to determine parts of an array and then apply a specified function to each independent part. Since present quantum computers are not robust enough for code writing and execution, we will build a model of a vector quantum computer that implements the Deutsch-Jozsa algorithm from a machine language view using the APL2 programming language. The core of the method is an operator (DJBOX) which allows evaluation of an arbitrary function f by the Deutsch-Jozsa algorithm. Two key functions of the method are GET_PARTITION and CALC_WITH_PARTITIONS. The GET_PARTITION function determines parts of an array based on the function f. The CALC_WITH_PARTITIONS function determines parts of an array based on the function f and then applies another function to each independent part. We will imagine the method is implemented on the above vector quantum computer. We will show that the method can be successfully executed.

  16. 33 CFR 165.556 - Regulated Navigation Area; Chesapeake and Delaware Canal, Chesapeake City Anchorage Basin, MD.

    Science.gov (United States)

    2010-07-01

    ... areas, found in 33 CFR 165.13, apply to the regulated navigation area described in paragraph (a) of this...; Chesapeake and Delaware Canal, Chesapeake City Anchorage Basin, MD. 165.556 Section 165.556 Navigation and..., Chesapeake City Anchorage Basin, MD. (a) Location. The following area is a regulated navigation area:...

  17. Thick-Walled Cylinder Theory Applied on a Conical Wedge Anchorage

    DEFF Research Database (Denmark)

    Bennitz, Anders; Grip, Niklas; Schmidt, Jacob Wittrup

    2011-01-01

    for further development of the anchorage.In this paper, we derive and examine an analytical model for the internal stresses and strains within the anchorage for a prescribed presetting distance. This model is derived from the theory of thick walled cylinders under the assumptions regarding plane stress......-symmetric and 3D FE (Finite Element) models. Analytical and FE axi-symmetric models show good correspondence, though compared to the 3D FE model, they show a clear difference in the predicted radial stress distribution on the FRP. Thus, the derived analytical model can be a useful and faster alternative to FE...

  18. STUDY ON CHARACTERISTICS OF RESIDUAL STRENGTH OF RC BEAMS WITH DEFECTIVE ANCHORAGES DUE TO CORROSION OF REINFORCEMENTS

    Science.gov (United States)

    Murakami, Yuki; Dong, Wei; Oshita, Hideki; Suzuki, Shuichi; Tsutsumi, Tomoaki

    In this study, to evaluate flexural strength and shear strength with def ective anchorages due to corrosion of reinforcemen t, the bending test of the RC beams r eceived damage in the anchorage region due to corrosion was carried out. As a result, it is se ems that the residual shear strength of RC beams with defective anchorages depends on shear span ratio in addition to the anchorage performance. Furthermore, the authors propose an evaluation model for an shear strength of RC beams with defective anchorages on the basis of these experimental results and analy tical result. The value of residual shear strength calculated using this model corresponds to the test results in the past.

  19. Pursuing Perfection: Distress and Interpersonal Functioning Among Adolescent Boys in Single-Sex and Co-Educational Independent Schools

    OpenAIRE

    Coren, Sidney A.; Luthar, Suniya S.

    2014-01-01

    This study extends past findings of heightened problems among affluent youth by examining adjustment patterns among boys in two academically elite, independent high schools: one for boys only and the other coeducational. Both samples manifested disproportionately high rates of internalizing and externalizing symptoms, but only the co-educational boys showed elevations in substance use. Boys in both schools showed elevations in a new outcome domain examined: exhibitionistic narcissism. Multiva...

  20. Functional Independence Measure in Iran: A Confirmatory Factor Analysis and Evaluation of Ceiling and Floor Effects in Traumatic Brain Injury Patients

    Directory of Open Access Journals (Sweden)

    Rezaei

    2015-12-01

    Full Text Available Background The functional independence measure (FIM is one of the most important assessment instruments for motor and cognitive dependence in rehabilitation medicine; however, there is little data about its confirmatory factor analysis (CFA and ceiling/floor effects from other countries and also in Iranian patients. Objectives The aim of this study was to evaluate a two-factor model (motor and cognitive independence as latent variables and ceiling/floor effects for FIM in Iranian patients with traumatic brain injuries (TBI. Patients and Methods In this cross-sectional study, 185 subacute TBI patients were selected from emergency and neurosurgery departments of Poursina Hospital (the largest trauma hospital in northern Iran, Rasht using the consecutive sampling method and were assessed for functional independence. Results The results of this study showed that the floor effect was not observed; however, ceiling effects were observed for the FIM total score and its subscales. The confirmatory factor analysis showed that the chi-square/df ratio was 2.8 for the two-factor structure and the fit indices for this structural model including root mean square error of approximation (RMSEA = 0.099, normed fit index (NFI = 0.96, tucker lewis index (TLI = 0.97, comparative fit index (CFI = 0.97 were close to standard indices. Conclusions Although ceiling effects should be considered for rehabilitation targets, the two-factor model of FIM (motor and cognitive independence has an eligible fitness for Iranian patients with TBI.

  1. Adults' perceptions of children's independence and other sex-role characteristics as a function of child's gender label.

    Science.gov (United States)

    Bell, N J; Hibbs, K; Milholland, T

    1978-12-01

    Male and female college students were presented with a photograph labeled as a 5-yr.-old boy or girl and heard statements attributed to the child. They then rated the child on sex-role traits and responded to open-ended questions about the child. The primary findings involved sex of child by sex of adult interactions on ratings of independence and leadership: in both cases, same-sex children were rated higher than opposite-sex children. There was also some evidence that women having high contact with children rated the child more extremely on opposite-sex traits than did those with little contact. PMID:740494

  2. Bishop Independence

    OpenAIRE

    Harris Liam H.; Perkins Stephanie; Roach Paul A.; Jones Siˆan K.

    2013-01-01

    Bishop Independence concerns determining the maximum number of Bishops that can be placed on a board such that no Bishop can attack any other Bishop. This paper presents the solution to the Bishop Independence problem, determining the Bishop Independence number, for all sizes of boards on the following topologies: the cylinder, the M¨obius strip, the torus, the Klein bottle and the surface of a cube.

  3. A generalized relation for solid-state drug stability as a function of excipient dilution: temperature-independent behavior.

    Science.gov (United States)

    Waterman, Kenneth C; Gerst, Paul; Dai, Zhen

    2012-11-01

    A proposed generalized relationship for the impact of excipients on the solid-state chemical stability of drug products is presented and shown to be consistent across multiple degradation products with two example drugs. In this model, when the number of drug particles is comparable to the number of excipient particles, the impact of the excipient on the degradant formation rate is independent of drug concentration. In contrast, when the number of drug particles is in excess of the number of excipient particles, a power-law relation (linear correlation between the logarithm of the degradant formation rate and the logarithm of the reciprocal of the drug concentration) is proposed based on a "quasi-liquid" model where drug particles fill in interstices between excipients. As predicted by this model, the experimental power-law lines have slopes of about 2/3 independent of temperature (0.61 ± 0.13 for n = 30 counting multiple degradation products and a range of temperatures and relative humidities for two drug products). PMID:22847821

  4. SLI-1 Cbl inhibits the engulfment of apoptotic cells in C. elegans through a ligase-independent function.

    Directory of Open Access Journals (Sweden)

    Courtney Anderson

    Full Text Available The engulfment of apoptotic cells is required for normal metazoan development and tissue remodeling. In Caenorhabditis elegans, two parallel and partially redundant conserved pathways act in cell-corpse engulfment. One pathway, which includes the small GTPase CED-10 Rac and the cytoskeletal regulator ABI-1, acts to rearrange the cytoskeleton of the engulfing cell. The CED-10 Rac pathway is also required for proper migration of the distal tip cells (DTCs during the development of the C. elegans gonad. The second pathway includes the receptor tyrosine kinase CED-1 and might recruit membranes to extend the surface of the engulfing cell. Cbl, the mammalian homolog of the C. elegans E3 ubiquitin ligase and adaptor protein SLI-1, interacts with Rac and Abi2 and modulates the actin cytoskeleton, suggesting it might act in engulfment. Our genetic studies indicate that SLI-1 inhibits apoptotic cell engulfment and DTC migration independently of the CED-10 Rac and CED-1 pathways. We found that the RING finger domain of SLI-1 is not essential to rescue the effects of SLI-1 deletion on cell migration, suggesting that its role in this process is ubiquitin ligase-independent. We propose that SLI-1 opposes the engulfment of apoptotic cells via a previously unidentified pathway.

  5. Arabidopsis CALCINEURIN B-LIKE10 Functions Independently of the SOS Pathway during Reproductive Development in Saline Conditions.

    Science.gov (United States)

    Monihan, Shea M; Magness, Courtney A; Yadegari, Ramin; Smith, Steven E; Schumaker, Karen S

    2016-05-01

    The accumulation of sodium in soil (saline conditions) negatively affects plant growth and development. The Salt Overly Sensitive (SOS) pathway in Arabidopsis (Arabidopsis thaliana) functions to remove sodium from the cytosol during vegetative development preventing its accumulation to toxic levels. In this pathway, the SOS3 and CALCINEURIN B-LIKE10 (CBL10) calcium sensors interact with the SOS2 protein kinase to activate sodium/proton exchange at the plasma membrane (SOS1) or vacuolar membrane. To determine if the same pathway functions during reproductive development in response to salt, fertility was analyzed in wild type and the SOS pathway mutants grown in saline conditions. In response to salt, CBL10 functions early in reproductive development before fertilization, while SOS1 functions mostly after fertilization when seed development begins. Neither SOS2 nor SOS3 function in reproductive development in response to salt. Loss of CBL10 function resulted in reduced anther dehiscence, shortened stamen filaments, and aborted pollen development. In addition, cbl10 mutant pistils could not sustain the growth of wild-type pollen tubes. These results suggest that CBL10 is critical for reproductive development in the presence of salt and that it functions in different pathways during vegetative and reproductive development.

  6. Plant Functional Diversity Can Be Independent of Species Diversity: Observations Based on the Impact of 4-Yrs of Nitrogen and Phosphorus Additions in an Alpine Meadow

    OpenAIRE

    Wei Li; Ji-Min Cheng; Kai-Liang Yu; Howard E. Epstein; Liang Guo; Guang-Hua Jing; Jie Zhao; Guo-Zhen Du

    2015-01-01

    Past studies have widely documented the decrease in species diversity in response to addition of nutrients, however functional diversity is often independent from species diversity. In this study, we conducted a field experiment to examine the effect of nitrogen and phosphorus fertilization ((NH4)2 HPO4) at 0, 15, 30 and 60 g m-2 yr-1 (F0, F15, F30 and F60) after 4 years of continuous fertilization on functional diversity and species diversity, and its relationship with productivity in an alp...

  7. Characterization of ambient fine particles in the northwestern area and Anchorage, Alaska.

    Science.gov (United States)

    Kim, Eugene; Hopke, Philip K

    2008-10-01

    Ambient PM2.5 (particulate matter less than 2.5 microm in aerodynamic diameter) in the northwestern United States and Alaska is dominated by carbonaceous compounds associated with wood burning and transportation sources. PM2.5 source characterization studies analyzing recent PM2.5 speciation data have not been previously reported for these areas. In this study, ambient PM2.5 speciation samples collected at two monitoring sites located in the northwestern area, Olympic Peninsula, WA, and Portland, OR, and one monitoring site located in Anchorage, AK, were characterized through source apportionments. Gasoline vehicle, secondary sulfate, and wood smoke were the largest sources of PM2.5 collected at the Anchorage, Olympic, and Portland monitoring sites, respectively. Secondary sulfates showed an April peak at Anchorage and a November peak at Portland that are likely related to the increased photochemical reaction and long-range transport in Anchorage and meteorological stagnation in Portland. Secondary nitrate at the Olympic site showed a weak summer high peak that could be caused by seasonal tourism in the national park. Backward trajectories suggested that the elevated aged sea salt concentrations at the Portland monitoring site could be regional transport of sea salt that passed through other contaminated air sheds along the coast. Oil combustion emissions that might originate from ships and ferries were observed at the Olympic monitoring site. PMID:18939780

  8. 75 FR 23804 - Notice of Inventory Completion: Alaska State Office, Bureau of Land Management, Anchorage, AK...

    Science.gov (United States)

    2010-05-04

    ... National Park Service Notice of Inventory Completion: Alaska State Office, Bureau of Land Management... completion of an inventory of human remains and associated funerary objects in the control of the U.S. Department of the Interior, Bureau of Land Management, Alaska State Office, Anchorage, AK, and in...

  9. 77 FR 5743 - Special Anchorage Areas; Port of New York, NY

    Science.gov (United States)

    2012-02-06

    ..., 2008, issue of the Federal Register (73 FR 3316). Public Meeting We do not now plan to hold a public... northern and southern special anchorage areas in Sheepshead Bay, NY; and reducing the illegal mooring of... transit of chartered fishing boats to and from their NYC-managed piers to the west. Since moored...

  10. Biofilm formation on surface characterized micro-implants for skeletal anchorage in orthodontics

    NARCIS (Netherlands)

    Chin, Yeen; Sandham, John; de Vries, Jacob; van der Mei, Henderina; Busscher, Hendrik

    2007-01-01

    Micro-implants are increasingly popular in clinical orthodontics to effect skeletal anchorage. However, biofilm formation on their surfaces and subsequent infection of peri-implant tissues can result in either exfoliation or surgical removal of these devices. The present study aimed to assess biofil

  11. 33 CFR 166.200 - Shipping safety fairways and anchorage areas, Gulf of Mexico.

    Science.gov (United States)

    2010-07-01

    ... Navigation or danger markings must be installed as required by 33 CFR Subchapter C. (c) Special Conditions...: For Federal Register citations affecting § 166.200, see the List of CFR Sections Affected, which... anchorage areas, Gulf of Mexico. 166.200 Section 166.200 Navigation and Navigable Waters COAST...

  12. Oxidative Stress Modulates DNA Methylation during Melanocyte Anchorage Blockade Associated with Malignant Transformation

    Directory of Open Access Journals (Sweden)

    Ana C.E. Campos

    2007-12-01

    Full Text Available Both oxidative/nitrosative stress and alterations in DNA methylation are observed during carcinogenesis of different tumor types, but no clear correlation between these events has been demonstrated until now. Melanoma cell lines were previously established after submitting the nontumorigenic melanocyte lineage, melan-a, to cycles of anchorage blockade. In this work, increased intracellular oxidative species and nitric oxide levels, as well as alterations in the DNA methylation, were observed after melan-a detachment, which were also associated with a decrease in intracellular homocysteine (Hey, an element in the methionine (universal methyl donor cycle. This alteration was accompanied by increase in glutathione (GSH levels and methylated DNA content. Furthermore, a significant increase in dnmti and 3b expression was identified along melan-a anchorage blockade. LG-Nitro-L-arginine methyl esther (L-NAME, known as a nitric oxide synthase (NOS inhibitor, and N-acetyl-L-cysteine (NAC prevented the increase in global DNA methylation, as well as the increase in dnmti and 3b expression, observed during melan-a detachment. Interestingly, both L-NAME and NAC did not inhibit nitric oxide (NO production in these cells, but abrogated superoxide anion production during anchorage blockade. In conclusion, oxidative stress observed during melanocyte anchorage blockade seems to modulate DNA methylation levels and may directly contribute to the acquisition of an anoikis-resistant phenotype through an epigenetic mechanism.

  13. Extraction treatment of an adult patient with severe bimaxillary dentoalveolar protrusion using microscrew anchorage

    Institute of Scientific and Technical Information of China (English)

    OUYANG Li; ZHOU Yan-heng; FU Min-kui; DING Peng

    2007-01-01

    @@ Bimaxillary dentoalveolar protrusion is one of the most prevalent malocclusion in Asian population,1 Traditionally, orthodontic treatment often involves the extraction of four first premolars2 and demands the least amount of anchorage loss, therefore headgear used to be an unavoid- able fate of these patients.

  14. Genome-scale functional analysis of the human genes modulating p53 activity by regulating MDM2 expression in a p53-independent manner.

    Science.gov (United States)

    Kim, Dong Min; Choi, Seung-Hyun; Yeom, Young Il; Min, Sang-Hyun; Kim, Il-Chul

    2016-09-16

    MDM2, a critical negative regulator of p53, is often overexpressed in leukemia, but few p53 mutations are found, suggesting that p53-independent MDM2 expression occurs due to alterations in MDM2 upstream regulators. In this study, a high MDM2 transcription level was observed (41.17%) regardless of p53 expression in patient with acute myeloid leukemia (AML). Therefore, we performed genome-scale functional screening of the human genes modulating MDM2 expression in a p53-independent manner. We searched co-expression profiles of genes showing a positive or negative pattern with MDM2 expression in a DNA microarray database, selected1089 links, and composed a screening library of 368 genes. Using MDM2 P1 and P2 promoter-reporter systems, we screened clones regulating MDM2 transcriptions in a p53-independent manner by overexpression. Nine clones from the screening library showed enhanced MDM2 promoter activity and MDM2 expression in p53-deficient HCT116 cells. Among them, six clones, including NTRK2, GNA15, SFRS2, EIF5A, ELAVL1, and YWHAB mediated MAPK signaling for expressing MDM2. These results indicate that p53-independent upregulation of MDM2 by increasing selected clones may lead to oncogenesis in AML and that MDM2-modulating genes are novel potential targets for AML treatment. PMID:27524244

  15. Fundamental Evaluation of Adaptation and Human Capabilities in a Condition Using a System to Give a User an Artificial Oculomotor Function to Control Directions of Both Eyes Independently

    Directory of Open Access Journals (Sweden)

    Fumio Mizuno

    2011-10-01

    Full Text Available To investigate flexible adaptation of visual system, we developed a system to provide a user an artificial oculomotor function to control directions of both eyes. The system named “Virtual Chameleon” consists of two CCD cameras independently controlled and a head-mounted display. The user can control each tracking directions of two cameras with sensors set to both hands so that the user can get independent arbitrary view fields for both eyes. We performed fundamental experiments to evaluate capability to evaluate adaptation to use of Virtual Chameleon and effects on the user's capabilities. Eleven healthy volunteers with normal and corrected-to-normal vision participated in the experiments. The experiments were tests to find out each position of targets put in both side of a subject. In the experiments, a condition using Virtual Chameleon and a condition without it was adopted. We obtained accuracy rates and time intervals to find out target positions as experimental results. The experiments showed all of volunteers became able to actively control independent visual axes and correctly understood two different views by using Virtual Chameleon, even though two independent view fields yielded binocular rivalry to volunteers and binocular rivalry reduced human capabilities compared to cases without Virtual Chameleon.

  16. Genome-scale functional analysis of the human genes modulating p53 activity by regulating MDM2 expression in a p53-independent manner.

    Science.gov (United States)

    Kim, Dong Min; Choi, Seung-Hyun; Yeom, Young Il; Min, Sang-Hyun; Kim, Il-Chul

    2016-09-16

    MDM2, a critical negative regulator of p53, is often overexpressed in leukemia, but few p53 mutations are found, suggesting that p53-independent MDM2 expression occurs due to alterations in MDM2 upstream regulators. In this study, a high MDM2 transcription level was observed (41.17%) regardless of p53 expression in patient with acute myeloid leukemia (AML). Therefore, we performed genome-scale functional screening of the human genes modulating MDM2 expression in a p53-independent manner. We searched co-expression profiles of genes showing a positive or negative pattern with MDM2 expression in a DNA microarray database, selected1089 links, and composed a screening library of 368 genes. Using MDM2 P1 and P2 promoter-reporter systems, we screened clones regulating MDM2 transcriptions in a p53-independent manner by overexpression. Nine clones from the screening library showed enhanced MDM2 promoter activity and MDM2 expression in p53-deficient HCT116 cells. Among them, six clones, including NTRK2, GNA15, SFRS2, EIF5A, ELAVL1, and YWHAB mediated MAPK signaling for expressing MDM2. These results indicate that p53-independent upregulation of MDM2 by increasing selected clones may lead to oncogenesis in AML and that MDM2-modulating genes are novel potential targets for AML treatment.

  17. Independence of Internal Auditors.

    Science.gov (United States)

    Montondon, Lucille; Meixner, Wilda F.

    1993-01-01

    A survey of 288 college and university auditors investigated patterns in their appointment, reporting, and supervisory practices as indicators of independence and objectivity. Results indicate a weakness in the positioning of internal auditing within institutions, possibly compromising auditor independence. Because the auditing function is…

  18. Peptidyl-Prolyl cis/trans Isomerase-Independent Functional NifH Mutant of Azotobacter vinelandii†

    OpenAIRE

    Gavini, Nara; Tungtur, Sudheer; Pulakat, Lakshmi

    2006-01-01

    Peptidyl-prolyl cis/trans isomerases (PPIases) play a pivotal role in catalyzing the correct folding of many prokaryotic and eukaryotic proteins that are implicated in a variety of biological functions, ranging from cell cycle regulation to bacterial infection. The nif accessory protein NifM, which is essential for the biogenesis of a functional NifH component of nitrogenase, is a PPIase. To understand the nature of the molecular signature that defines the NifM dependence of NifH, we screened...

  19. Complement activation by PEG-functionalized multi-walled carbon nanotubes is independent of PEG molecular mass and surface density

    DEFF Research Database (Denmark)

    Andersen, Alina Joukainen; Windschiegl, Barbara; Ilbasmis-Tamer, Sibel;

    2013-01-01

    eliminated. Our observations address the difficulty in making carbon nanotubes more compatible with innate immunity through covalent PEG functionalization as well as double PEGylation strategies. From the Clinical EditorComplement-mediated toxicity is a major limiting factor in certain nanomedicine...

  20. The 2-Minute Step Test is Independently Associated with Cognitive Function in Older Adults with Heart Failure

    Science.gov (United States)

    Alosco, Michael L.; Spitznagel, Mary Beth; Raz, Naftali; Cohen, Ronald; Sweet, Lawrence H.; Colbert, Lisa H.; Josephson, Richard; Waechter, Donna; Hughes, Joel; Rosneck, Jim; Gunstad, John

    2016-01-01

    Background and Aims Cognitive impairment is common in persons with heart failure (HF), and measures like the 6-minute walk test (6MWT) are known to correspond to level of impairment. The 2-minute step test (2MST) has been suggested as a more practical alternative to the 6MWT, though no study has examined whether it is associated with cognitive impairment in persons with HF. This study examined whether the 2MST is associated with cognitive function in older adults with HF. Methods Older adults with HF (N = 145; 68.97±9.31 years) completed the 2MST and a neuropsychological test battery that assessed function in multiple cognitive domains. Results Consistent with past work, HF patients exhibited high rates of cognitive impairment. Hierarchical regression analyses adjusting for demographic and medical characteistics found that the 2MST accounted for unique variance in global cognitive function (ΔR2 = .09, p tests indicated that better 2MST performance was significantly correlated with better global, attention, executive, and language test performance. Conclusion The current results indicate that the 2MST is associated with cognitive function in older adults with HF. Further work is needed to clarify underlying mechanisms for this association and the value of implementing the 2MST during routine visits. PMID:22182711

  1. Independent and Combined Effects of Exercise and Vitamin D on Muscle Morphology, Function and Falls in the Elderly

    Directory of Open Access Journals (Sweden)

    Robin M. Daly

    2010-09-01

    Full Text Available Regular exercise, particularly progressive resistance training (PRT, is recognized as one of the most effective strategies to prevent age-related muscle loss (sarcopenia, but its effects on muscle function are mixed. However, emerging data indicates that high velocity PRT (fast concentric muscle contractions is more effective for improving functional outcomes than traditional PRT. In terms of falls prevention, high-challenging balance training programs appear to be most effective. There is also compelling evidence that supplemental vitamin D is an effective therapeutic option for falls prevention. The findings from a recent meta-analysis revealed that supplemental vitamin D at a dose of at least 700–1,000 IU/d or an achieved serum 25(OHD level of at least 60 nmol/L was associated with reduced falls risk among older individuals. Based on these findings, it is possible that the combination of exercise and vitamin D could have a synergistic effect on muscle morphology and function, particularly since both interventions have been shown to have beneficial effects on type II “fast twitch” muscle fibers and systemic inflammation, which have both been linked to losses in muscle mass and function. Unfortunately however, the findings from the limited number of factorial 2 × 2 design RCTs indicate that additional vitamin D does not enhance the effects of exercise on measures of muscle morphology, function or falls risk. However, none of these trials were adequately powered to detect a “synergistic” effect between the two treatment strategies, but it is likely that if an exercise-by-vitamin D interaction does exist, it may be limited to situations when vitamin D deficiency/insufficiency is corrected. Further targeted research in “high risk” groups is still needed to address this question, and evaluate whether there is a threshold level of serum 25(OHD to maximize the effects of exercise on muscle and falls risk.

  2. Extraction of fast neuronal changes from multichannel functional near-infrared spectroscopy signals using independent component analysis

    Science.gov (United States)

    Morren, Geert; Wolf, Martin; Lemmerling, Philippe; Wolf, Ursula; Choi, Jee H.; Gratton, Enrico; De Lathauwer, Lieven; Van Huffel, Sabine

    2002-06-01

    Fast changes in the range of milliseconds in the optical properties of cerebral tissue, which are associated with brain activity, can be detected using non-invasive near-infrared spectroscopy (NIRS). These changes in light scattering are due to an alteration in the refractive index at neuronal membranes. The aim of this study was to develop highly sensitive data analysis algorithms to detect this fast signal, which is small compared to other physiological signals. A frequency-domain tissue oximeter, whose laser diodes were modulated at 110MHz was used. The amplitude, mean intensity and phase of the modulated optical signal was measured at 96Hz sample rate. The probe consisting of 4 crossed source detector pairs was placed above the motor cortex, contralateral to the hand performing a tapping exercise consisting of alternating rest- and tapping periods of 20s each. The tapping frequency, which was set to 3.55Hz or 2.5 times the heart rate of the subject to avoid the influence of harmonics on the signal, could not be observed in any of the individual signals measured by the detectors. An adaptive filter was used to remove the arterial pulsatility from the optical signals. Independent Component Analysis allowed to separate signal components in which the tapping frequency was clearly visible.

  3. Exercise training and antioxidant supplementation independently improve cognitive function in adult male and female GFAP-APOE mice

    Directory of Open Access Journals (Sweden)

    Kiran Chaudhari

    2014-09-01

    Conclusion: Exercise was the most effective treatment at improving cognitive function in both genotypes and sex, while antioxidants seemed to be effective only in the APOE4. In young adult mice only non-spatial learning and memory were improved. The combination of the two treatments did not yield further improvement in cognition, and there was no antagonistic action of the antioxidant supplementation on the beneficial effects of exercise.

  4. The Elucidation of the Interactome of 16 Arabidopsis bZIP Factors Reveals Three Independent Functional Networks

    Science.gov (United States)

    Llorca, Carles Marco; Berendzen, Kenneth Wayne; Malik, Waqas Ahmed; Mahn, Stefan; Piepho, Hans-Peter; Zentgraf, Ulrike

    2015-01-01

    The function of the bZIP transcription factors is strictly dependent on their ability to dimerize. Heterodimerization has proven to be highly specific and is postulated to operate as a combinatorial mechanism allowing the generation of a large variety of dimers with unique qualities by specifically combining a small set of monomers; an assumption that has not yet been tested systematically. Here, the interaction pattern and the transactivation properties of 16 Arabidopsis thaliana bZIPs are examined in transiently transformed Arabidopsis protoplasts to deliver a perspective on the relationship between bZIP dimerization and function. An interaction matrix of bZIPs belonging to the C, G, H, and S1 bZIP groups was resolved by Bimolecular Fluorescent Complementation (BiFC) coupled to quantitative flow cytometric analysis, while an extensive GUS reporter gene assay was carried out to determine the effect of different bZIP pairs on the expression of four different known bZIP-targeted promoters. Statistical data treatment and complementary bioinformatic analysis were performed to substantiate the biological findings. According to these results, the 16 bZIPs interact in three isolated networks, within which their members dimerize non-specifically and exhibit a significant level of functional redundancy. A coherent explanation for these results is supported by in silico analysis of differences in the length, structure and composition of their leucine zippers and appears to explain their dimerization specificity and dynamics observed in vivo quite well. A model in which the bZIP networks act as functional units is proposed. PMID:26452049

  5. HDAC3 as a molecular chaperone for shuttling phosphorylated TR2 to PML: a novel deacetylase activity-independent function of HDAC3.

    Directory of Open Access Journals (Sweden)

    Pawan Gupta

    Full Text Available TR2 is an orphan nuclear receptor specifically expressed in early embryos (Wei and Hsu, 1994, and a transcription factor for transcriptional regulation of important genes in stem cells including the gate keeper Oct4 (Park et al. 2007. TR2 is known to function as an activator (Wei et al. 2000, or a repressor (Chinpaisal et al., 1998, Gupta et al. 2007. Due to the lack of specific ligands, mechanisms triggering its activator or repressor function have remained puzzling for decades. Recently, we found that all-trans retinoic acid (atRA triggers the activation of extracellular-signal-regulated kinase 2 (ERK2, which phosphorylates TR2 and stimulates its partitioning to promyelocytic leukemia (PML nuclear bodies, thereby converting the activator function of TR2 into repression (Gupta et al. 2008; Park et al. 2007. Recruitment of TR2 to PML is a crucial step in the conversion of TR2 from an activator to a repressor. However, it is unclear how phosphorylated TR2 is recruited to PML, an essential step in converting TR2 from an activator to a repressor. In the present study, we use both in vitro and in vivo systems to address the problem of recruiting TR2 to PML nuclear bodies. First, we identify histone deacetylase 3 (HDAC3 as an effector molecule. HDAC3 is known to interact with TR2 (Franco et al. 2001 and this interaction is enhanced by the atRA-stimulated phosphorylation of TR2 at Thr-210 (Gupta et al. 2008. Secondly, in this study, we also find that the carrier function of HDAC3 is independent of its deacetylase activity. Thirdly, we find another novel activity of atRA that stimulates nuclear enrichment of HDAC3 to form nuclear complex with PML, which is ERK2 independent. This is the first report identifying a deacetylase-independent function for HDAC3, which serves as a specific carrier molecule that targets a specifically phosphorylated protein to PML NBs. This is also the first study delineating how protein recruitment to PML nuclear bodies occurs

  6. Pulmonary Artery Stiffness Is Independently Associated with Right Ventricular Mass and Function: A Cardiac MR Imaging Study.

    Science.gov (United States)

    Dawes, Timothy J W; Gandhi, Ajay; de Marvao, Antonio; Buzaco, Rui; Tokarczuk, Paweł; Quinlan, Marina; Durighel, Giuliana; Diamond, Tamara; Monje Garcia, Laura; de Cesare, Alain; Cook, Stuart A; O'Regan, Declan P

    2016-08-01

    Purpose To determine the relationship between pulmonary artery (PA) stiffness and both right ventricular (RV) mass and function with cardiac magnetic resonance (MR) imaging. Materials and Methods The study was approved by the local research ethics committee, and all participants gave written informed consent. Cardiac MR imaging was performed at 1.5 T in 156 healthy volunteers (63% women; age range, 19-61 years; mean age, 36.1 years). High-temporal-resolution phase-contrast imaging was performed in the main and right PAs. Pulmonary pulse wave velocity (PWV) was determined by the interval between arterial systolic upslopes. RV function was assessed with feature tracking to derive peak systolic strain and strain rate, as well as peak early-diastolic strain rate. RV volumes, ejection fraction (RVEF), and mass were measured from the cine images. The association of pulmonary PWV with RV function and mass was quantified with univariate linear regression. Interstudy repeatability was assessed with intraclass correlation. Results The repeatability coefficient for pulmonary PWV was 0.96. Increases in pulmonary PWV and RVEF were associated with increases in age (r = 0.32, P Online supplemental material is available for this article. PMID:26909648

  7. The influence of age on health valuations: the older olds prefer functional independence while the younger olds prefer less morbidity

    Directory of Open Access Journals (Sweden)

    Hofman CS

    2015-07-01

    Full Text Available Cynthia S Hofman,1,2 Peter Makai,1 Han Boter,3 Bianca M Buurman,4 Anton J de Craen,5 Marcel GM Olde Rikkert,1 Rogier Donders,2 René JF Melis1 1Department of Geriatric Medicine, Radboud University Medical Center, Nijmegen, the Netherlands; 2Department for Health Evidence, Radboud University Medical Center, Nijmegen, the Netherlands; 3Department of Epidemiology, University of Groningen, University Medical Centre Groningen, Groningen, the Netherlands; 4Department of Internal Medicine, Section of Geriatric Medicine, Academic Medical Center, Amsterdam, the Netherlands; 5Department of Gerontology and Geriatrics, Leiden University Medical Centre, Leiden, the Netherlands Background: To assess the effectiveness of geriatric interventions, The Older Persons and Informal Caregivers Survey – Composite Endpoint (TOPICS-CEP has been developed based on health valuations of older persons and informal caregivers. This study explored the influence of the raters’ age on the preference weights of TOPICS-CEP’s components.Methods: A vignette study was conducted with 200 raters (mean age ± standard deviation: 72.5±11.8 years; 66.5% female. Profiles of older persons were used to obtain the preference weights for all TOPICS-CEP components: morbidity, functional limitations, emotional wellbeing, pain experience, cognitive functioning, social functioning, self-perceived health, and self-perceived quality of life. The raters assessed the general wellbeing of these vignettes on a 0–10 scale. Mixed linear regression analysis with interaction terms was used to explore the effects of raters’ age on the preference weights.Results: Interaction effects between age and the TOPICS-CEP components showed that older raters gave significantly (P<0.05 more weight to functional limitations and social functioning and less to morbidities and pain experience, compared to younger raters.Conclusion: Researchers examining effectiveness in elderly care need to consider the

  8. Diastolic dysfunction is an independent predictor of cardiovascular events in incident dialysis patients with preserved systolic function.

    Directory of Open Access Journals (Sweden)

    Jae Hyun Han

    Full Text Available Diastolic heart failure (HF, the prevalence of which is gradually increasing, is associated with cardiovascular (CV morbidity and mortality in the general population and, more specifically, in patients with end-stage renal disease (ESRD. However, the impact of diastolic dysfunction on CV outcomes has not been studied in incident dialysis patients with preserved systolic function.This prospective observational cohort study investigates the clinical consequence of diastolic dysfunction and the predictive power of diastolic echocardiographic parameters for CV events in 194 incident ESRD patients with normal or near normal systolic function, who started dialysis between July 2008 and August 2012.During a mean follow-up duration of 27.2 months, 57 patients (29.4% experienced CV events. Compared to the CV event-free group, patients with CV events had a significantly higher left ventricular (LV mass index, ratio of early mitral flow velocity (E to early mitral annulus velocity (E' (E/E', LA volume index (LAVI, deceleration time, and right ventricular systolic pressure, and a significantly lower LV ejection fraction and E'. In multivariate Cox proportional hazard analysis, E/E'>15 and LAVI>32 mL/m2 significantly predicted CV events (E/E'>15: hazard ratio [HR] = 5.40, 95% confidence interval [CI] = 2.73-10.70, P32 mL/m2: HR = 5.56, 95% CI = 2.28-13.59, P15 and LAVI>32 mL/m2 had the worst CV outcomes.An increase in E/E' or LAVI is a significant risk factor for CV events in incident dialysis patients with preserved LV systolic function.

  9. Blood oxygen depletion is independent of dive function in a deep diving vertebrate, the northern elephant seal.

    Directory of Open Access Journals (Sweden)

    Jessica U Meir

    Full Text Available Although energetics is fundamental to animal ecology, traditional methods of determining metabolic rate are neither direct nor instantaneous. Recently, continuous blood oxygen (O2 measurements were used to assess energy expenditure in diving elephant seals (Mirounga angustirostris, demonstrating that an exceptional hypoxemic tolerance and exquisite management of blood O2 stores underlie the extraordinary diving capability of this consummate diver. As the detailed relationship of energy expenditure and dive behavior remains unknown, we integrated behavior, ecology, and physiology to characterize the costs of different types of dives of elephant seals. Elephant seal dive profiles were analyzed and O2 utilization was classified according to dive type (overall function of dive: transit, foraging, food processing/rest. This is the first account linking behavior at this level with in vivo blood O2 measurements in an animal freely diving at sea, allowing us to assess patterns of O2 utilization and energy expenditure between various behaviors and activities in an animal in the wild. In routine dives of elephant seals, the blood O2 store was significantly depleted to a similar range irrespective of dive function, suggesting that all dive types have equal costs in terms of blood O2 depletion. Here, we present the first physiological evidence that all dive types have similarly high blood O2 demands, supporting an energy balance strategy achieved by devoting one major task to a given dive, thereby separating dive functions into distinct dive types. This strategy may optimize O2 store utilization and recovery, consequently maximizing time underwater and allowing these animals to take full advantage of their underwater resources. This approach may be important to optimizing energy expenditure throughout a dive bout or at-sea foraging trip and is well suited to the lifestyle of an elephant seal, which spends > 90% of its time at sea submerged making diving its

  10. Coronary Flow Reserve Predicts Cardiopulmonary Fitness in Patients with Coronary Artery Disease Independently of Systolic and Diastolic Function

    DEFF Research Database (Denmark)

    Snoer, Martin; Olsen, Rasmus Huan; Monk-Hansen, Tea;

    2014-01-01

    Aims Despite revascularization and optimal medical treatment, patients with coronary artery disease (CAD) have reduced exercise capacity. In the absence of coronary artery stenosis, coronary flow reserve (CFR) is a measure of coronary microvascular function, and a marker of future poor outcome...... early (E) and late (A) inflow velocities, and tissue Doppler diastolic (e′) and systolic (s′) velocities. Peak coronary flow velocity (CFV) was measured in the LAD using pulse-wave Doppler. CFR was calculated as the ratio between peak CFV at rest and during vasodilator stress. Median CFR was 2.22 (1...

  11. BMP-2 functions independently of SHH signaling and triggers cell condensation and apoptosis in regenerating axolotl limbs

    Directory of Open Access Journals (Sweden)

    Finnson Kenneth

    2010-02-01

    Full Text Available Abstract Background Axolotls have the unique ability, among vertebrates, to perfectly regenerate complex body parts, such as limbs, after amputation. In addition, axolotls pattern developing and regenerating autopods from the anterior to posterior axis instead of posterior to anterior like all tetrapods studied to date. Sonic hedgehog is important in establishing this anterior-posterior axis of limbs in all tetrapods including axolotls. Interestingly, its expression is conserved (to the posterior side of limb buds and blastemas in axolotl limbs as in other tetrapods. It has been suggested that BMP-2 may be the secondary mediator of sonic hedgehog, although there is mounting evidence to the contrary in mice. Since BMP-2 expression is on the anterior portion of developing and regenerating limbs prior to digit patterning, opposite to the expression of sonic hedgehog, we examined whether BMP-2 expression was dependent on sonic hedgehog signaling and whether it affects patterning of the autopod during regeneration. Results The expression of BMP-2 and SOX-9 in developing and regenerating axolotl limbs corresponded to the first digits forming in the anterior portion of the autopods. The inhibition of sonic hedgehog signaling with cyclopamine caused hypomorphic limbs (during development and regeneration but did not affect the expression of BMP-2 and SOX-9. Overexpression of BMP-2 in regenerating limbs caused a loss of digits. Overexpression of Noggin (BMP inhibitor in regenerating limbs also resulted in a loss of digits. Histological analysis indicated that the loss due to BMP-2 overexpression was the result of increased cell condensation and apoptosis while the loss caused by Noggin was due to a decrease in cell division. Conclusion The expression of BMP-2 and its target SOX-9 was independent of sonic hedgehog signaling in developing and regenerating limbs. Their expression correlated with chondrogenesis and the appearance of skeletal elements has

  12. Using the Deutsch-Jozsa algorithm to determine parts of an array and apply a specified function to each independent part

    OpenAIRE

    Lipovaca, Samir

    2009-01-01

    Using the Deutsch-Jozsa algorithm, we will develop a method for solving a class of problems in which we need to determine parts of an array and then apply a specified function to each independent part. Since present quantum computers are not robust enough for code writing and execution, we will build a model of a vector quantum computer that implements the Deutsch-Jozsa algorithm from a machine language view using the APL2 programming language. The core of the method is an operator (DJBOX) wh...

  13. Kidney transplantation improves arterial function measured by pulse wave analysis and endothelium-independent dilatation in uraemic patients despite deterioration of glucose metabolism

    DEFF Research Database (Denmark)

    Hornum, Mads; Clausen, Peter; Idorn, Thomas;

    2011-01-01

    BACKGROUND: The aim of this study is to investigate the effect of kidney transplantation on arterial function in relation to changes in glucose metabolism. METHODS: Included were 40 kidney recipients (Tx group, age 38 ± 13 years) and 40 patients without known diabetes remaining on the waiting list...... (endothelium dependent) and after nitroglycerin administration (endothelium independent). The insulin resistance was estimated by the insulin sensitivity index (ISI). RESULTS: AIX was reduced from 27% (17-33) to 14% (7-25) (P = 0.01) after 1 year in the Tx group and remained stable in uraemic controls (P = 0...

  14. Anchorage of machined and TPS-coated dental implants of various lengths: An in vivo study in the dog maxilla

    OpenAIRE

    Carrel, Jean-Pierre; Szmukler-Moncler, Serge; Bernard, Jean-Pierre; Belser, Urs Christophe; Vazquez, Lydia

    2013-01-01

    The use of short implants is nowadays frequent in daily practice. The objective of this experimental study was to test the correlation between extremely different implant surfaces and the anchorage of short implants

  15. Application of a New Anchorage towards the Flexural Strengthening of RC Rectangular Beams with External Steel Tendons

    Directory of Open Access Journals (Sweden)

    Sungnam Hong

    2016-04-01

    Full Text Available To strengthen concrete beams, a new anchorage was proposed, and its performance was evaluated in this study. Seven concrete beams were manufactured and flexurally loaded with displacement control up to the failure point. As important test variables, the anchorage type (new/conventional and the prestress levels in the steel rebar (0, 50, and 100 kN were selected. To investigate the strengthening effects based on these test variables, the deflection, strain, and failure mode were recorded, and then the load, ductility index, and energy ratio were analyzed. Test results showed that the newly proposed end anchorage had better strengthening effects and a greater inelastic energy than the conventional end anchorages.

  16. Anchorage onto deciduous teeth: effectiveness of early rapid maxillary expansion in increasing dental arch dimension and improving anterior crowding

    OpenAIRE

    Mutinelli, Sabrina; Manfredi, Mario; Guiducci, Antonio; Denotti, Gloria; Cozzani, Mauro

    2015-01-01

    Background Anchorage onto permanent dentition is a common procedure in rapid maxillary expansion. However, replacing first permanent molars with the second deciduous molars seems to be an option to reduce some negative side effects during orthodontic treatment. The purpose of this study was to evaluate the dental effect of rapid maxillary expansion with anchorage exclusively onto deciduous teeth performed in the first period of transition. Methods Twenty patients with a lateral cross-bite tre...

  17. Post-exercise contractility, diastolic function, and pressure: Operator-independent sensor-based intelligent monitoring for heart failure telemedicine

    Directory of Open Access Journals (Sweden)

    Giannoni Massimo

    2009-05-01

    Full Text Available Abstract Background New sensors for intelligent remote monitoring of the heart should be developed. Recently, a cutaneous force-frequency relation recording system has been validated based on heart sound amplitude and timing variations at increasing heart rates. Aim To assess sensor-based post-exercise contractility, diastolic function and pressure in normal and diseased hearts as a model of a wireless telemedicine system. Methods We enrolled 150 patients and 22 controls referred for exercise-stress echocardiography, age 55 ± 18 years. The sensor was attached in the precordial region by an ECG electrode. Stress and recovery contractility were derived by first heart sound amplitude vibration changes; diastolic times were acquired continuously. Systemic pressure changes were quantitatively documented by second heart sound recording. Results Interpretable sensor recordings were obtained in all patients (feasibility = 100%. Post-exercise contractility overshoot (defined as increase > 10% of recovery contractility vs exercise value was more frequent in patients than controls (27% vs 8%, p 1 in 20 patients and in none of the controls (p 1 in only 3 patients (p Conclusion Post-exercise contractility, diastolic time and pressure changes can be continuously measured by a cutaneous sensor. Heart disease affects not only exercise systolic performance, but also post-exercise recovery, diastolic time intervals and blood pressure changes – in our study, all of these were monitored by a non-invasive wearable sensor.

  18. Conditional and Unconditional Statistical Independence

    OpenAIRE

    Phillips, Peter C. B.

    1987-01-01

    Conditional independence almost everywhere in the space of the conditioning variates does not imply unconditional independence, although it may well imply unconditional independence of certain functions of the variables. An example that is important in linear regression theory is discussed in detail. This involves orthogonal projections on random linear manifolds, which are conditionally independent but not unconditionally independent under normality. Necessary and sufficient conditions are o...

  19. Choosing Independence

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    Milo Djukanovic, Prime Minister of Montenegro, won a key referendum May 21 when voters in his tiny, mountainous nation endorsed a plan to split from Serbia and become an independent state. This marked a final step in the breakup of the former Yugoslavia formed by six republics.

  20. A genetic algorithm for dynamic parameters reverse deduction of integrated anchorage system

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    In the analysis of the system of anchoring bar and wall rock in small strain and longitudinal vibration dynamic response, the influence of the cement grouting as well as the rock layer on the anchor bar can be evaluated as the two kinds of parameters: the dynamic stiffness and the damp, which are the vital reference of the anchorage quality. Based on the analytic solution to the dynamic equation of the integrated anchor bar, the new approach which combines genetic algorithm and the toolbox of Matlab is applied to solve the problem of multi-parameters reverse deduction for integrated anchorage system in dynamic testing. Using the traits of the self-organizing, self-adapting and the fast convergence speed of the genetic algorithm, the optimum of all possible solutions to dynamic parameters is obtained by calculating the project instances. Examples show that the method presented in this paper is effective and reliable.

  1. Impedance-Based Cable Force Monitoring in Tendon-Anchorage Using Portable PZT-Interface Technique

    Directory of Open Access Journals (Sweden)

    Thanh-Canh Huynh

    2014-01-01

    Full Text Available In this paper, a portable PZT interface for tension force monitoring in the cable-anchorage subsystem is developed. Firstly, the theoretical background of the impedance-based method is presented. A few damage evaluation approaches are outlined to quantify the variation of impedance signatures. Secondly, a portable PZT interface is designed to monitor impedance signatures from the cable-anchorage subsystem. One degree-of-freedom analytical model of the PZT interface is established to explain how to represent the loss of cable force from the change in the electromechanical impedance of the PZT interface as well as reducing the sensitive frequency band by implementing the interface device. Finally, the applicability of the proposed PZT-interface technique is experimentally evaluated for cable force-loss monitoring in a lab-scaled test structure.

  2. Inclusion of CFRP-Epoxy Composite for End Anchorage in NSM-Epoxy Strengthened Beams

    Directory of Open Access Journals (Sweden)

    Md. Akter Hosen

    2015-01-01

    Full Text Available Nowadays, the use of near surface mounted (NSM technique strengthening reinforced concrete (RC structural members is going very popular. The failure modes of NSM strengthened reinforced concrete (RC beams have been shown to be largely due to premature failure such as concrete cover separation. In this study, CFRP U-wrap end anchorage with CFRP fabrics was used to eliminate the concrete cover separation failure. A total of eight RC rectangular beam specimens of 125 mm width, 250 mm depth, and 2300 mm length were tested. One specimen was kept unstrengthened as a reference; three specimens were strengthened with NSM steel bars and the remaining four specimens were strengthened with NSM steel bars together with the U-wrap end anchorage. The experimental results showed that wrapped strengthened beams had higher flexural strength and superior ductility performance. The results also show that these beams had less deflection, strain, crack width, and spacing.

  3. The Arabidopsis downy mildew resistance gene, RPP13-Nd, functions independently of NDR1 and EDS1 and does not require the accumulation of salicylic acid.

    Science.gov (United States)

    Bittner-Eddy, P D; Beynon, J L

    2001-03-01

    RPP13-Nd-mediated resistance prevents parasitism by five isolates of Peronospora parasitica (At) in a transgenic Arabidopsis. Columbia background. We tested the effect of a number of known disease resistance mutations on the RPP13-Nd function and found that resistance remained unaltered in plants carrying mutations in either EDS1 or NDR1 and in double ndr1-1/eds1-2 mutant lines. Furthermore, we found that pbs2, pad4-1, npr1-1, and rps5-1, which compromise resistance to a number of P. parasitica (At) isolates, had no affect on RPP13-Nd function. In addition, RPP13-Nd-mediated resistance remained unchanged in a background of salicylic acid depletion (nahG). We conclude that RPP13-Nd is the first Arabidopsis R gene product reported to act via a novel signaling pathway that is independent of salicylic acid-mediated responses and is completely independent of NDR1 and EDS1.

  4. PP2A(Cdc55)'s role in reductional chromosome segregation during achiasmate meiosis in budding yeast is independent of its FEAR function.

    Science.gov (United States)

    Kerr, Gary W; Wong, Jin Huei; Arumugam, Prakash

    2016-01-01

    PP2A(Cdc55) is a highly conserved serine-threonine protein phosphatase that is involved in diverse cellular processes. In budding yeast, meiotic cells lacking PP2A(Cdc55) activity undergo a premature exit from meiosis I which results in a failure to form bipolar spindles and divide nuclei. This defect is largely due to its role in negatively regulating the Cdc Fourteen Early Anaphase Release (FEAR) pathway. PP2A(Cdc55) prevents nucleolar release of the Cdk (Cyclin-dependent kinase)-antagonising phosphatase Cdc14 by counteracting phosphorylation of the nucleolar protein Net1 by Cdk. CDC55 was identified in a genetic screen for monopolins performed by isolating suppressors of spo11Δ spo12Δ lethality suggesting that Cdc55 might have a role in meiotic chromosome segregation. We investigated this possibility by isolating cdc55 alleles that suppress spo11Δ spo12Δ lethality and show that this suppression is independent of PP2A(Cdc55)'s FEAR function. Although the suppressor mutations in cdc55 affect reductional chromosome segregation in the absence of recombination, they have no effect on chromosome segregation during wild type meiosis. We suggest that Cdc55 is required for reductional chromosome segregation during achiasmate meiosis and this is independent of its FEAR function. PMID:27455870

  5. PP2ACdc55’s role in reductional chromosome segregation during achiasmate meiosis in budding yeast is independent of its FEAR function

    Science.gov (United States)

    Kerr, Gary W.; Wong, Jin Huei; Arumugam, Prakash

    2016-01-01

    PP2ACdc55 is a highly conserved serine-threonine protein phosphatase that is involved in diverse cellular processes. In budding yeast, meiotic cells lacking PP2ACdc55 activity undergo a premature exit from meiosis I which results in a failure to form bipolar spindles and divide nuclei. This defect is largely due to its role in negatively regulating the Cdc Fourteen Early Anaphase Release (FEAR) pathway. PP2ACdc55 prevents nucleolar release of the Cdk (Cyclin-dependent kinase)-antagonising phosphatase Cdc14 by counteracting phosphorylation of the nucleolar protein Net1 by Cdk. CDC55 was identified in a genetic screen for monopolins performed by isolating suppressors of spo11Δ spo12Δ lethality suggesting that Cdc55 might have a role in meiotic chromosome segregation. We investigated this possibility by isolating cdc55 alleles that suppress spo11Δ spo12Δ lethality and show that this suppression is independent of PP2ACdc55’s FEAR function. Although the suppressor mutations in cdc55 affect reductional chromosome segregation in the absence of recombination, they have no effect on chromosome segregation during wild type meiosis. We suggest that Cdc55 is required for reductional chromosome segregation during achiasmate meiosis and this is independent of its FEAR function. PMID:27455870

  6. Higher Cognitive Function in Elderly Individuals with Previous Cataract Surgery: Cross-Sectional Association Independent of Visual Acuity in the HEIJO-KYO Cohort.

    Science.gov (United States)

    Miyata, Kimie; Obayashi, Kenji; Saeki, Keigo; Tone, Nobuhiro; Tanaka, Kunihiko; Nishi, Tomo; Morikawa, Masayuki; Kurumatani, Norio; Ogata, Nahoko

    2016-06-01

    Cataract surgery improves visual acuity and drastically increases the capacity for light reception to the retina. Although previous studies suggested that both light exposure and visual acuity were associated with cognitive function, the relationships between cataract surgery, visual acuity, and cognitive function have not been evaluated in large populations. In this cross-sectional study, we measured cognitive function using the Mini-Mental State Examination and best-corrected visual acuity in pseudophakic (previous cataract surgery) and phakic (no previous cataract surgery) elderly individuals. Of 945 participants (mean age 71.7 years), 166 (17.6%) had pseudophakia and 317 (33.5%) had impaired cognitive function (score ≤26). The pseudophakic group showed significantly better visual acuity than the phakic group (p = 0.003) and lower age-adjusted odds ratio (ORs) for cognitive impairment (OR 0.66; p = 0.038). Consistently, in multivariate logistic regression models, after adjusting for confounding factors, including visual acuity and socioeconomic status, ORs for cognitive impairment were significantly lower in the pseudophakic group than in the phakic group (OR 0.64; 95% confidence interval 0.43-0.96; p = 0.031). This association remained significant in sensitivity analysis, excluding participants with low cognitive score ≤23 (n = 36). In conclusion, in a general elderly population, prevalence of cognitive impairment was significantly lower in pseudophakic individuals independently of visual acuity. The association was also independent of several major causes of cognitive impairment such as aging, gender, obesity, socioeconomic status, hypertension, diabetes, sleep disturbances, depressive symptoms, and physical inactivity.

  7. Impedance monitoring at tendon-anchorage via mountable PZT interface and temperature-effect compensation

    Science.gov (United States)

    Huynh, Thanh-Canh; Nguyen, Tuan-Cuong; Choi, Sang-Hoon; Kim, Jeong-Tae

    2016-04-01

    In this study, the pre-stress force in pre-stressed concrete (PSC) girders is monitored via mountable PZT interface under varying temperature. Firstly, an impedance-based technique using mountable PZT interface is proposed for pre-stress-loss monitoring in tendon-anchorage systems. A cross correlation-based temperature-effect compensation algorithm using an effective frequency shift (EFS) of impedance signatures is visited. Secondly, lab-scale experiments are performed on a PSC girder instrumented with a mountable PZT interface at tendon-anchorage. A series of temperature variation and pre-stress-loss events are simulated for the lab-scale PSC girder. Thirdly, the feasibility of the mountable PZT interface for pre-stress-loss monitoring in tendon-anchorage is experimentally verified under constant temperature conditions. Finally, the PZT interface device is examined for pre-stress-loss monitoring under temperature changes to validate its applicability. The temperature effect on impedance signatures is compensated by minimizing cross-correlation deviation between impedance patterns of the mountable PZT interface.

  8. Independent preferences

    DEFF Research Database (Denmark)

    Vind, Karl

    1991-01-01

    A simple mathematical result characterizing a subset of a product set is proved and used to obtain additive representations of preferences. The additivity consequences of independence assumptions are obtained for preferences which are not total or transitive. This means that most of the economic ...... theory based on additive preferences - expected utility, discounted utility - has been generalized to preferences which are not total or transitive. Other economic applications of the theorem are given......A simple mathematical result characterizing a subset of a product set is proved and used to obtain additive representations of preferences. The additivity consequences of independence assumptions are obtained for preferences which are not total or transitive. This means that most of the economic...

  9. Are Independent Fiscal Institutions Really Independent?

    Directory of Open Access Journals (Sweden)

    Slawomir Franek

    2015-08-01

    Full Text Available In the last decade the number of independent fiscal institutions (known also as fiscal councils has tripled. They play an important oversight role over fiscal policy-making in democratic societies, especially as they seek to restore public finance stability in the wake of the recent financial crisis. Although common functions of such institutions include a role in analysis of fiscal policy, forecasting, monitoring compliance with fiscal rules or costing of spending proposals, their roles, resources and structures vary considerably across countries. The aim of the article is to determine the degree of independence of such institutions based on the analysis of the independence index of independent fiscal institutions. The analysis of this index values may be useful to determine the relations between the degree of independence of fiscal councils and fiscal performance of particular countries. The data used to calculate the index values will be derived from European Commission and IMF, which collect sets of information about characteristics of activity of fiscal councils.

  10. The LKB1 tumor suppressor differentially affects anchorage independent growth of HPV positive cervical cancer cell lines

    International Nuclear Information System (INIS)

    Infection with high-risk human papillomaviruses is causally linked to cervical carcinogenesis. However, most lesions caused by high-risk HPV infections do not progress to cancer. Host cell mutations contribute to malignant progression but the molecular nature of such mutations is unknown. Based on a previous study that reported an association between liver kinase B1 (LKB1) tumor suppressor loss and poor outcome in cervical cancer, we sought to determine the molecular basis for this observation. LKB1-negative cervical and lung cancer cells were reconstituted with wild type or kinase defective LKB1 mutants and we examined the importance of LKB1 catalytic activity in known LKB1-regulated processes including inhibition of cell proliferation and elevated resistance to energy stress. Our studies revealed marked differences in the biological activities of two kinase defective LKB1 mutants in the various cell lines. Thus, our results suggest that LKB1 may be a cell-type specific tumor suppressor. - Highlights: • LKB1 is a tumor suppressor that is linked to Peutz-Jeghers syndrome. • Peutz-Jeghers syndrome patients have a high incidence of cervical cancer. • Cervical cancer is caused by HPV infections. • This study investigates LKB1 tumor suppressor activity in cervical cancer

  11. The LKB1 tumor suppressor differentially affects anchorage independent growth of HPV positive cervical cancer cell lines

    Energy Technology Data Exchange (ETDEWEB)

    Mack, Hildegard I.D.; Munger, Karl, E-mail: kmunger@rics.bwh.harvard.edu

    2013-11-15

    Infection with high-risk human papillomaviruses is causally linked to cervical carcinogenesis. However, most lesions caused by high-risk HPV infections do not progress to cancer. Host cell mutations contribute to malignant progression but the molecular nature of such mutations is unknown. Based on a previous study that reported an association between liver kinase B1 (LKB1) tumor suppressor loss and poor outcome in cervical cancer, we sought to determine the molecular basis for this observation. LKB1-negative cervical and lung cancer cells were reconstituted with wild type or kinase defective LKB1 mutants and we examined the importance of LKB1 catalytic activity in known LKB1-regulated processes including inhibition of cell proliferation and elevated resistance to energy stress. Our studies revealed marked differences in the biological activities of two kinase defective LKB1 mutants in the various cell lines. Thus, our results suggest that LKB1 may be a cell-type specific tumor suppressor. - Highlights: • LKB1 is a tumor suppressor that is linked to Peutz-Jeghers syndrome. • Peutz-Jeghers syndrome patients have a high incidence of cervical cancer. • Cervical cancer is caused by HPV infections. • This study investigates LKB1 tumor suppressor activity in cervical cancer.

  12. Comparative evaluation of sagittal anchorage loss in lingual and labial appliances during space closure: A pilot study

    Directory of Open Access Journals (Sweden)

    Shivanand Venkatesh

    2015-01-01

    Full Text Available Aim: The purpose of this investigation was to assess and compare the anchorage loss between labial and lingual appliance systems during space closure. Materials and Methods: Twenty subjects were part of the study among which 10 subjects (mean age 21 ± 3.6 years were treated using lingual appliance system (0.018" slot-STb™ and 10 subjects (mean age 19 ± 6.1 years were treated using labial preadjusted edgewise appliance system (0.018" slot-MBT™ . First premolar extractions were performed to enable retraction of anterior teeth. Lateral cephalometric radiographs were taken at two intervals, before starting space closure and after space closure that were connoted as T0 and T1 and were analyzed using the method described by Pancherz to measure anchorage loss. Intraclass correlation coefficient (ICC was used to evaluate intraexaminer reliability of the measurements. Student′s t-test was performed to verify any statistical significant correlation between the labial and lingual appliance systems. Statistical differences were determined at the 95% confidence level (P < 0.05. Results: The results showed that all ICC for lingual and labial group were ≥0.90 showing good repeatability of the measurements. Mean anchorage loss of 1.238 ± 0.17 mm in lingual appliance system and an anchorage loss of 2.06 ± 0.39 mm occurred with the labial appliance system. On the comparison between the two appliance systems, lingual appliance demonstrated a significantly lesser anchorage loss than did the labial appliance. Interpretation and Conclusion: This prospective study concludes with the fact that lingual appliance provided better anchorage control than labial appliance during space closure. Use of lingual appliance could be considered in critical anchorage cases when compared with labial appliance.

  13. Very low levels of microalbuminuria are associated with increased risk of coronary heart disease and death independently of renal function, hypertension, and diabetes

    DEFF Research Database (Denmark)

    Klausen, Klaus; Borch-Johnsen, Knut; Feldt-Rasmussen, Bo;

    2004-01-01

    level of urinary albumin excretion is unknown. It is also unknown whether impaired renal function confounds the association. METHODS AND RESULTS: In the Third Copenhagen City Heart Study in 1992 to 1994, 2762 men and women 30 to 70 years of age underwent a detailed cardiovascular investigation program...... excretion above the upper quartile, ie, 4.8 microg/min, was associated with increased risk of coronary heart disease (RR, 2.0; 95% CI, 1.4 to 3.0; P<0.001) and death (RR, 1.9; 95% CI, 1.5 to 2.4; P<0.001) independently of age, sex, renal creatinine clearance, diabetes mellitus, hypertension, and plasma...

  14. Plasma soluble urokinase-type plasminogen activator receptor level is independently associated with coronary microvascular function in patients with non-obstructive coronary artery disease

    DEFF Research Database (Denmark)

    Mekonnen, Girum; Corban, Michel T; Hung, Olivia Y;

    2015-01-01

    BACKGROUND: Soluble urokinase-type plasminogen activator receptor (suPAR) is a novel biomarker released from leukocytes and endothelial cells that has been associated with atherosclerotic cardiovascular disease. We hypothesized that plasma suPAR level is an independent predictor of coronary...... microvascular function. METHODS: Coronary blood flow velocity and plasma suPAR levels were evaluated in patients with non-obstructive coronary artery disease. Coronary flow reserve (CFR) was calculated as the ratio of hyperemic to basal average peak blood flow velocity and coronary microvascular dysfunction was...... defined as CFR ≤ 2.0 in the setting of a fractional flow reserve value of ≥0.75. Plasma suPAR levels were measured using ELISA technique. The association between suPAR and CFR was investigated using univariate and multivariate regression analyses. RESULTS: In 66 patients, 47% were men, 26% had diabetes...

  15. 'Independence' Panorama

    Science.gov (United States)

    2005-01-01

    [figure removed for brevity, see original site] Click on the image for 'Independence' Panorama (QTVR) This is the Spirit 'Independence' panorama, acquired on martian days, or sols, 536 to 543 (July 6 to 13, 2005), from a position in the 'Columbia Hills' near the summit of 'Husband Hill.' The summit of 'Husband Hill' is the peak near the right side of this panorama and is about 100 meters (328 feet) away from the rover and about 30 meters (98 feet) higher in elevation. The rocky outcrops downhill and on the left side of this mosaic include 'Larry's Lookout' and 'Cumberland Ridge,' which Spirit explored in April, May, and June of 2005. The panorama spans 360 degrees and consists of 108 individual images, each acquired with five filters of the rover's panoramic camera. The approximate true color of the mosaic was generated using the camera's 750-, 530-, and 480-nanometer filters. During the 8 martian days, or sols, that it took to acquire this image, the lighting varied considerably, partly because of imaging at different times of sol, and partly because of small sol-to-sol variations in the dustiness of the atmosphere. These slight changes produced some image seams and rock shadows. These seams have been eliminated from the sky portion of the mosaic to better simulate the vista a person standing on Mars would see. However, it is often not possible or practical to smooth out such seams for regions of rock, soil, rover tracks or solar panels. Such is the nature of acquiring and assembling large panoramas from the rovers.

  16. EFFECT OF END ANCHORAGE LENGTH AND STIRRUP RATIO ON BOND AND SHEAR CAPACITY OF CONCRETE BEAMS WITH NONMETALLIC REINFORCEMENT

    Directory of Open Access Journals (Sweden)

    RENDY THAMRIN

    2016-06-01

    Full Text Available This paper presents a study on the effect of end anchorage length and stirrup ratio on bond and shear capacity of concrete beams reinforced with Carbon Fiber Reinforced Polymer (CFRP bars. This study was carried out using test data on nine simply supported reinforced concrete beams with stirrups. The beams were subjected to two point monotonic loads and the test variables were the length of end anchorage and the stirrup ratio. Theoretical equations for calculating bond strength and shear capacity obtained from literature were applied and then compared with experimental values. Beams with inadequate end anchorage length showed premature bond failure even when there was sufficient stirrup ratio. Beams with adequate end anchorage length failed in shear or flexure modes depending on the stirrup ratio. A numerical model for bond stress and slip in response to pullout forces was also used to determine analytically the bond stress distributions along end anchorage. The behavior of tensile force acting on the stirrups was also examined. Finally, a simple model for predicting tension force acting on the stirrups was proposed.

  17. Murine Herc6 Plays a Critical Role in Protein ISGylation In Vivo and Has an ISGylation-Independent Function in Seminal Vesicles.

    Science.gov (United States)

    Arimoto, Kei-ichiro; Hishiki, Takayuki; Kiyonari, Hiroshi; Abe, Takaya; Cheng, Chuyi; Yan, Ming; Fan, Jun-Bao; Futakuchi, Mitsuru; Tsuda, Hiroyuki; Murakami, Yoshiki; Suzuki, Hideyuki; Zhang, Dong-Er; Shimotohno, Kunitada

    2015-05-01

    ISG15 conjugation (ISGylation) to proteins is a multistep process involving interferon (IFN)-inducible UBE1L (E1), UbcH8 (E2), and ISG15 E3 ligases (E3s). Studies performed over the past several years have shown that ISGylation plays a pivotal role in the host antiviral response against certain viruses. Recent in vitro studies revealed that human Herc5 and mouse Herc6 are major ISG15 E3 ligases, respectively. However, the global function of Herc5/6 proteins in vivo still remains unclear. Here, we report generation and initial characterization of Herc6 knockout mice. Substantial reductions of ISGylation were observed in Herc6-deficient cells after polyinosinic-polycytidylic acid double-stranded RNA injection of mice or IFN treatment of cells. On the other hand, Herc6-deficient cells and wild-type (WT) cells had similar responses to IFN stimulation, Sendai virus (Z strain) infection, and vesicular stomatitis virus infection. These results indicate that Herc6 does not play a critical role in antiviral defense of these viral infections in mice. Interestingly, male Herc6-deficient mice showed seminal vesicle hypertrophy. No such problem was detected in WT and ISG15 activating enzyme Ube1L-deficient mice. These results suggest that in addition to promoting protein ISGylation, Herc6 has a novel and protein ISGylation-independent function in the male reproductive system.

  18. Bi-functional prodrugs of 5-aminolevulinic acid and butyric acid increase erythropoiesis in anemic mice in an erythropoietin-independent manner.

    Science.gov (United States)

    Rephaeli, Ada; Tarasenko, Nataly; Fibach, Eitan; Rozic, Gabriela; Lubin, Ido; Lipovetsky, Julia; Furman, Svetlana; Malik, Zvi; Nudelman, Abraham

    2016-08-25

    Anemia is a major cause of morbidity and mortality worldwide resulting from a wide variety of pathological conditions. In severe cases it is treated by blood transfusions or injection of erythroid stimulating agents, e.g., erythropoietin (Epo), which can be associated with serious adverse effects. Therefore, there is a need to develop new treatment modalities. We recently reported that treatment of erythroleukemic cells with the novel the bi-functional prodrugs of 5-aminolevulinic acid (ALA) and butyric acid (BA), AN233 and AN908, enhanced hemoglobin (Hb) synthesis to a substantially higher level than did ALA and BA individually or their mixture. Herein, we describe that these prodrugs when given orally to mice induced histone deacetylase inhibition in the kidneys, bone marrow and spleen, thus, indicating good penetrability to the tissues. In mice where anemia was chemically induced, treatment with the prodrugs increased the Hb, the number of red blood cells (RBCs) and the percentage of reticulocytes to normal levels. The prodrugs had no adverse effects even after repeated treatment at 100-200mg/kg for 50days. The lack of increased levels of Epo in the blood of mice that were treated with the prodrugs suggests that AN233 and AN908 affected the Hb and RBC levels in an Epo-independent manner. Taken together with our previous studies, we propose that the prodrugs increase globin expression by BA inhibition of histone deacetylase and elevation heme synthesis by ALA. These results support an Epo-independent approach for treating anemia with these prodrugs. PMID:27283485

  19. Importin α3/Qip1 is involved in multiplication of mutant influenza virus with alanine mutation at amino acid 9 independently of nuclear transport function.

    Directory of Open Access Journals (Sweden)

    Yutaka Sasaki

    Full Text Available The nucleoprotein (NP of influenza A virus is transported into the nucleus via the classical importin α/β pathway, and proceeds via nuclear localization signals (NLSs recognized by importin α molecules. Although NP binds to importin α isoforms Rch1, Qip1 and NPI-1, the role of each individual isoform during the nuclear transport of NP and replication of the influenza virus remains unknown. In this study, we examined the contribution of importin α isoforms for nuclear localization of NP and viral growth using a panel of NP mutants containing serial alanine replacements within an unconventional NLS of NP. Alanine mutation at amino acid 8 (R8A caused a significant reduction in the nuclear localization and binding to the three importin isoforms. The R8A NP mutant virus did not generate by reverse-genetics approach. This indicates that position 8 is the main site that mediates nuclear localization via interactions with Rch1, Qip1 and NPI-1, and subsequent viral production. This was confirmed by the finding that the conservation of amino acid 8 in human- and avian-origin influenza virus NP was necessary for virus propagation. By contrast, another mutant, S9A NP, which localized in the nucleus, caused a reduction in viral growth and vRNA transcription, suggesting that the unconventional NLS within NP may be associated with nuclear transport, vRNA transcription and viral replication through independent pathways. Interestingly, the N-terminal 110-amino acid region, which contained the unconventional NLS with S9A mutation, mainly bound to Qip1. Furthermore, activities of vRNA transcription and replication of S9A NP mutants were decreased by silencing Qip1 in without changing nuclear localization, indicating that Qip1 involves in multiplication of S9A mutant virus independently of nuclear transport function. Collectively, our results demonstrate the unconventional NLS within NP might have the additional ability to regulate the viral replication that

  20. Verification of the local structural response of building structures in the anchorage areas of heavy components

    International Nuclear Information System (INIS)

    In conventional dynamic structural analyses for determining dynamic system response for various locations at which components are installed inside the structures it is common practice (in order to simplify analytical effort) to assume that the anchorage (anchor plate, anchor bolts or throughbolts, concrete and reinforcement in the area of bound) has rigid body characteristics and that the building structure itself does not display any local response of its own. The influence of the stiffness of the anchor plate as well anchor bolts and its stress level on the dynamic response is also neglected. For a large number of anchoring systems, especially for all those components and systems having only a small mass, this assumption is certainly appropriate. At some locations, particularly at points where heavy components are anchored or when loading input has been increased, this can lead to local loading of the anchor system as well as of the building structure well into the nonlinear range. Often, verification of capability to accommodate these loads is not possible without changing the wall thicknesses or increasing the percentage of reinforcement. Since the presence of linear or nonlinear effects can be expected to result in energy dissipation (increase in damping capacity and also a change in the stiffness of the coupled system) it must be assumed that the dynamic response between the theoretical coupling point A and the real connection point B of the component on the anchor plate can be considerably altered. Some changes of the dynamic response in the connection point B have to be expected generally even in cases of linear-elastic loading of the anchorage. Using typical anchoring systems as an example, the influence of consideration of nonlinear effects in the anchorage area of a typical anchor plate on the dynamic response as well as the conservatism of conventional analytical approaches are investigated

  1. Detection of bond failure in the anchorage zone of reinforced concrete beams via acoustic emission monitoring

    Science.gov (United States)

    Abouhussien, Ahmed A.; Hassan, Assem A. A.

    2016-07-01

    In this study, acoustic emission (AE) monitoring was utilised to identify the onset of bond failure in reinforced concrete beams. Beam anchorage specimens were designed and tested to fail in bond in the anchorage zone. The specimens included four 250 × 250 × 1500 mm beams with four variable bonded lengths (100, 200, 300, and 400 mm). Meanwhile, an additional 250 × 250 × 2440 mm beam, with 200 mm bonded length, was tested to investigate the influence of sensor location on the identification of bond damage. All beams were tested under four-point loading setup and continuously monitored using three distributed AE sensors. These attached sensors were exploited to record AE signals resulting from both cracking and bond deterioration until failure. The variations in the number of AE hits and cumulative signal strength (CSS) versus test time were evaluated to achieve early detection of crack growth and bar slippage. In addition, AE intensity analysis was performed on signal strength of collected AE signals to develop two additional parameters: historic index (H (t)) and severity (S r). The analysis of these AE parameters enabled an early detection of both first cracks (at almost the mid-span of the beam) and bar slip in either of the anchorage zones at the beams’ end before their visual observation, regardless of sensor location. The results also demonstrated a clear correlation between the damage level in terms of crack development/measured free end bar slip and AE parameters (number of hits, CSS, H(t), and S r).

  2. Impact of a new gasoline benzene regulation on ambient air pollutants in Anchorage, Alaska

    Science.gov (United States)

    Yano, Yuriko; Morris, Stephen S.; Salerno, Christopher; Schlapia, Anne M.; Stichick, Mathew

    2016-05-01

    The purpose of this study was to quantify the impact of a new U.S. Environmental Protection Agency (EPA) standard that limits the amount of benzene allowed in gasoline on ambient benzene concentrations. This new standard, together with two companion regulations that limit cold-temperature automotive emissions and the permeability of portable fuel containers, was expected to lower the levels of ambient benzene and other volatile organic compounds (VOCs) nationwide. In this study the impact of the gasoline benzene standard was evaluated in Anchorage, Alaska in a two-phase ambient air monitoring study conducted before and after the new gasoline standard was implemented. Gasoline sold by Anchorage retailers was also evaluated in each phase to determine the content of benzene and other gasoline components. The average benzene content in Anchorage gasoline was reduced by 70%, from 5.05% (w/w) to 1.53% (w/w) following the implementation of the standard. The annual mean ambient benzene concentration fell by 51%, from 0.99 ppbv in Phase 1 to 0.49 ppbv in Phase 2. Analysis suggests the change in gasoline benzene content alone reduced benzene emissions by 46%. The changes in toluene, ethylbenzene, and xylene content in gasoline between Phase 1 and 2 were relatively small and the differences in the mean ambient concentrations of these compounds between phases were modest. Our results suggest that cold winter communities in high latitude and mountainous regions may benefit more from the gasoline benzene standard because of high benzene emissions resulting from vehicle cold start and a tendency to develop atmospheric stagnation conditions in the winter.

  3. Concentrations of fecal coliform bacteria in creeks, Anchorage, Alaska, August and September 1998

    Science.gov (United States)

    Dorava, Joseph M.; Love, Andra

    1999-01-01

    Water samples were collected from five creeks in undeveloped, semi-developed, and developed areas of Anchorage, Alaska, during August and September 1998 to determine concentrations of fecal coliform bacteria. In undeveloped areas of Ship, Chester, and Campbell Creeks, and the semi-developed area of Rabbit Creek, concentrations of fecal coliform bacteria ranged from less than 1 to 16 colonies per 100 milliliters of water. In the semi-developed area of Little Rabbit Creek, concentrations ranged from 30 to 860 colonies per 100 milliliters of water. In developed areas of the creeks, concentrations of fecal coliform bacteria ranged from 6 to 80 colonies per 100 milliliters of water.

  4. Dental Extrusion with Orthodontic Miniscrew Anchorage: A Case Report Describing a Modified Method

    Directory of Open Access Journals (Sweden)

    Ricardo Fidos Horliana

    2015-01-01

    Full Text Available In recent years, the skeletal anchorage through miniscrews has expanded the treatment options in orthodontics (Yamaguchi et al., 2012. We hereby present a modified method for tooth extrusion for cases where crown-lengthening surgery is contraindicated for aesthetic reasons. This modified method uses three orthodontic appliances: a mini-implant, an orthodontic wire, and a bracket. The aim of this case report was to increase the length of the clinical crown of a fractured tooth (tooth 23 by means of an orthodontic extrusion with the modified method of Roth and Diedrich.

  5. Wind loads analysis at the anchorages of the Talavera de la Reina cable stayed bridge

    Directory of Open Access Journals (Sweden)

    L. Rosa

    2014-06-01

    Full Text Available This paper describes wind tunnel tests performed on wind tunnel models of the Talavera de la Reina cable stayed bridge. The work describes the aeroelastic model construction and it is focused on the evaluation and analysis of the mean and peak wind loads at the tower foundation and the cable anchorages since these data can be very useful by the bridge manufacturer as a support for the bridge design. The work is part of a complete wind tunnel study carried out to analyze the aeroelastic stability of the bridge.

  6. RNA Polymerase V Functions in Arabidopsis Interphase Heterochromatin Organization Independently of the 24-nt siRNA-Directed DNA Methylation Pathway

    Institute of Scientific and Technical Information of China (English)

    Olga Pontes; Pedro Costa-Nunes; Paul Vithayathil; Craig S.Pikaard

    2009-01-01

    In Arabidopsis,pericentromeric repeats,retroelements,and silenced rRNA genes are assembled into heterochromatin within nuclear structures known as chromocenters.The mechanisms governing higher-order heterochromatin organization are poorly understood but 24-nt small interfering RNAs (siRNAs) are known to play key roles in heterochromatin formation.Nuclear RNA polymerase IV (Pol IV),RNA-DEPENDENT RNA POLYMERASE 2 (RDR2),and DICER-LIKE 3 (DCL3) are required for biogenesis of 24-nt siRNAs that associate with ARGONAUTE 4 (AGO4).Nuclear RNA polymerase V (Pol V) collaborates with DRD1 (DEFICIENT IN RNA-DEPENDENT DNA METHYLATION 1) to generate transcripts at heterochromatic loci that are hypothesized to bind to siRNA-AGO4 complexes and subsequently recruit the de-novo DNA methylation and/or histone modifying machinery.Here,we report that decondensation of the major pericentromeric repeats and depletion of the heterochromatic mark histone H3 lysine 9 dimethylation at chromocenters occurs specifically in pol V and drd1 mutants.Disruption of pericentromeric repeats condensation is coincident with transcriptional reactivation of specific classes of pericentromeric 180-bp repeats.We further demonstrate that Pol V functions independently of Pol IV,RDR2,and DCL3-mediated siRNA production to affect interphase heterochromatin organization,possibly by involving RNAs that recruit structural or chromatin-modifying proteins.

  7. Constitutively-active androgen receptor variants function independently of the HSP90 chaperone but do not confer resistance to HSP90 inhibitors.

    Science.gov (United States)

    Gillis, Joanna L; Selth, Luke A; Centenera, Margaret M; Townley, Scott L; Sun, Shihua; Plymate, Stephen R; Tilley, Wayne D; Butler, Lisa M

    2013-05-01

    The development of lethal, castration resistant prostate cancer is associated with adaptive changes to the androgen receptor (AR), including the emergence of mutant receptors and truncated, constitutively active AR variants. AR relies on the molecular chaperone HSP90 for its function in both normal and malignant prostate cells, but the requirement for HSP90 in environments with aberrant AR expression is largely unknown. Here, we investigated the efficacy of three HSP90 inhibitors, 17-AAG, HSP990 and AUY922, against clinically-relevant AR missense mutants and truncated variants. HSP90 inhibition effectively suppressed the signaling of wild-type AR and all AR missense mutants tested. By contrast, two truncated AR variants, AR-V7 and ARv567es, exhibited marked resistance to HSP90 inhibitors. Supporting this observation, nuclear localization of the truncated AR variants was not affected by HSP90 inhibition and AR variant:HSP90 complexes could not be detected in prostate cancer cells. Interestingly, HSP90 inhibition resulted in accumulation of AR-V7 and ARv567es in both cell lines and human tumor explants. Despite the apparent independence of AR variants from HSP90 and their treatment-associated induction, the growth of cell lines with endogenous or enforced expression of AR-V7 or ARv567es remained highly sensitive to AUY922. This study demonstrates that functional AR variant signaling does not confer resistance to HSP90 inhibition, yields insight into the interaction between AR and HSP90 and provides further impetus for the clinical application of HSP90 inhibitors in advanced prostate cancer.

  8. Self-assembled peptide-based hydrogels as scaffolds for anchorage-dependent cells.

    Science.gov (United States)

    Zhou, Mi; Smith, Andrew M; Das, Apurba K; Hodson, Nigel W; Collins, Richard F; Ulijn, Rein V; Gough, Julie E

    2009-05-01

    We report here the design of a biomimetic nanofibrous hydrogel as a 3D-scaffold for anchorage-dependent cells. The peptide-based bioactive hydrogel is formed through molecular self-assembly and the building blocks are a mixture of two aromatic short peptide derivatives: Fmoc-FF (Fluorenylmethoxycarbonyl-diphenylalanine) and Fmoc-RGD (arginine-glycine-aspartate) as the simplest self-assembling moieties reported so far for the construction of small-molecule-based bioactive hydrogels. This hydrogel provides a highly hydrated, stiff and nanofibrous hydrogel network that uniquely presents bioactive ligands at the fibre surface; therefore it mimics certain essential features of the extracellular matrix. The RGD sequence as part of the Fmoc-RGD building block plays a dual role of a structural component and a biological ligand. Spectroscopic and imaging analysis using CD, FTIR, fluorescence, TEM and AFM confirmed that FF and RGD peptide sequences self-assemble into beta-sheets interlocked by pi-pi stacking of the Fmoc groups. This generates the cylindrical nanofibres interwoven within the hydrogel with the presence of RGDs in tunable densities on the fibre surfaces. This rapid gelling material was observed to promote adhesion of encapsulated dermal fibroblasts through specific RGD-integrin binding, with subsequent cell spreading and proliferation; therefore it may offer an economical model scaffold to 3D-culture other anchorage-dependent cells for in-vitro tissue regeneration. PMID:19201459

  9. Behaviour of Plate Anchorage in Plate-Reinforced Composite Coupling Beams

    Directory of Open Access Journals (Sweden)

    W. Y. Lam

    2013-01-01

    Full Text Available As a new alternative design, plate-reinforced composite (PRC coupling beam achieves enhanced strength and ductility by embedding a vertical steel plate into a conventionally reinforced concrete (RC coupling beam. Based on a nonlinear finite element model developed in the authors’ previous study, a parametric study presented in this paper has been carried out to investigate the influence of several key parameters on the overall performance of PRC coupling beams. The effects of steel plate geometry, span-to-depth ratio of beams, and steel reinforcement ratios at beam spans and in wall regions are quantified. It is found that the anchorage length of the steel plate is primarily controlled by the span-to-depth ratio of the beam. Based on the numerical results, a design curve is proposed for determining the anchorage length of the steel plate. The load-carrying capacity of short PRC coupling beams with high steel ratio is found to be controlled by the steel ratio of wall piers. The maximum shear stress of PRC coupling beams should be limited to 15 MPa.

  10. Behaviour of plate anchorage in plate-reinforced composite coupling beams.

    Science.gov (United States)

    Lam, W Y; Li, Lingzhi; Su, R K L; Pam, H J

    2013-01-01

    As a new alternative design, plate-reinforced composite (PRC) coupling beam achieves enhanced strength and ductility by embedding a vertical steel plate into a conventionally reinforced concrete (RC) coupling beam. Based on a nonlinear finite element model developed in the authors' previous study, a parametric study presented in this paper has been carried out to investigate the influence of several key parameters on the overall performance of PRC coupling beams. The effects of steel plate geometry, span-to-depth ratio of beams, and steel reinforcement ratios at beam spans and in wall regions are quantified. It is found that the anchorage length of the steel plate is primarily controlled by the span-to-depth ratio of the beam. Based on the numerical results, a design curve is proposed for determining the anchorage length of the steel plate. The load-carrying capacity of short PRC coupling beams with high steel ratio is found to be controlled by the steel ratio of wall piers. The maximum shear stress of PRC coupling beams should be limited to 15 MPa. PMID:24288465

  11. Consolidated results of the AECB staff's detailed review of Ontario Hydro Nuclear's integrated independent performance assessment and safety system functional inspection findings

    International Nuclear Information System (INIS)

    In January 1997, Ontario Hydro announced that a team of nuclear industry experts from the United States, called the Nuclear Performance Advisory Group (NPAG), had been employed to help manage its nuclear program and to implement needed improvements in Ontario Hydro Nuclear (OHN) operations. In the spring of 1997, NPAG initiated a series of detailed reviews of OHN's operations, at its Pickering, Bruce and Darlington nuclear generating stations and in OHN's Head Office groups. These reviews, called 'Independent Integrated Performance Assessments' (UPA) and 'Safety System Functional Inspections' (SSFI), were carried out in April and May of this year with the objective of developing 'an integrated, accurate, and comprehensive understanding of the performance of OHN'.They were conducted in response to a request from the President of Ontario Hydro for a 'brutally honest' assessment of Ontario Hydro Nuclear. NPAG has since been integrated into Ontario Hydro's line organization. Ontario Hydro's Board of Directors received the results of the IIPAs and SSFIs and an Ontario Hydro report titled Basis for Continued Operation on August 12, 1997. AECB staff has been reviewing the reports containing these results since receiving them at that time. The conclusions of these studies are extremely critical of the management of Ontario Hydro Nuclear. They identify a large number of shortcomings in the operation and maintenance of the nuclear generating stations. Ontario Hydro states in the IIPA and SSFI reports that the reports are, by design, negative in slant and emphasize the weaknesses in performance rather than the strengths. The reports conclude that the stations can continue to operate safety while the near and long term improvements are implemented. This report contains the consolidated results of the AECB staffs detailed review of the IIPA and SSFI findings. (author)

  12. A Comparison between Two Instruments for Assessing Dependency in Daily Activities: Agreement of the Northwick Park Dependency Score with the Functional Independence Measure

    Directory of Open Access Journals (Sweden)

    Siv Svensson

    2012-01-01

    Full Text Available Background. There is a need for tools to assess dependency among persons with severe impairments. Objectives. The aim was to compare the Functional Independence Measure (FIM and the Northwick Park Dependency Score (NPDS, in a sample from in-patient rehabilitation. Material and Methods. Data from 115 persons (20 to 65 years of age with neurological impairments was gathered. Analyses were made of sensitivity, specificity, positive predictive value, and negative predictive value. Agreement of the scales was assessed with kappa and concordance with Goodman-Kruskal’s gamma. Scale structures were explored using the Rank-Transformable Pattern of Agreement (RTPA. Content validation was performed. Results. The sensitivity of the NPDS as compared to FIM varied between 0.53 (feeding and 1.0 (mobility and specificity between 0.64 (mobility and 1.0 (bladder. The positive predictive value varied from 0.62 (mobility to 1.0 (bladder, and the negative predictive value varied from 0.48 (bowel to 1.0 (mobility. Agreement between the scales was moderate to good (four items and excellent (three items. Concordance was good, with a gamma of −.856, an asymptotic error (ase of .025, and P<.000. The parallel reliability between the FIM and the NPDS showed a tendency for NPDS to be more sensitive (having more categories when dependency is high. Conclusion. FIM and NPDS complement each other. NPDS can be used as a measure for severely injured patients who are sensitive when there is a high need of nursing time.

  13. 33 CFR 165.110 - Safety and Security Zone; Liquefied Natural Gas Carrier Transits and Anchorage Operations, Boston...

    Science.gov (United States)

    2010-07-01

    ... United States within the Captain of the Port (COTP) Boston zone, as defined in 33 CFR 3.05-10, two miles... listed in 33 CFR 150.490 and falling within the waters of the Boston COTP Zone, as defined in 33 CFR 3.05...; Liquefied Natural Gas Carrier Transits and Anchorage Operations, Boston, Massachusetts. 165.110 Section...

  14. The "Real Self" and Inauthenticity: The Importance of Self-Concept Anchorage for Emotional Experiences in the Workplace

    Science.gov (United States)

    Sloan, Melissa M.

    2007-01-01

    I examine the utility of self-concept anchorage (as described by Turner 1976) in the analysis of inauthenticity in the workplace. As controlling internally felt emotion may distance the worker from her true feelings or true self, the management of emotion in the workplace can produce feelings of inauthenticity in the worker. This relationship has…

  15. Granger Independent Martingale Processes

    OpenAIRE

    Cherubini, Umberto; Gobbi, Fabio; Mulinacci, Sabrina; Romagnoli, Silvia

    2016-01-01

    We introduce a new class of processes for the evaluation of multivariate equity derivatives. The proposed setting is well suited for the application of the standard copula function theory to processes, rather than variables, and easily enables to enforce the martingale pricing requirement. The martingale condition is imposed in a general multidimensional Markov setting to which we only add the restriction of no-Granger-causality of the increments (Granger-independent increments). We call this...

  16. Independence Logic and Abstract Independence Relations

    OpenAIRE

    Paolini, Gianluca

    2014-01-01

    We continue the work on the relations between independence logic and the model-theoretic analysis of independence, generalizing the results of [15] and [16] to the framework of abstract independence relations for an arbitrary AEC. We give a model-theoretic interpretation of the independence atom and characterize under which conditions we can prove a completeness result with respect to the deductive system that axiomatizes independence in team semantics and statistics.

  17. Monitoring and Management System Design of the Anchorage at the Upstream of the Three Gorges Dam%三峡船闸上游锚地监控管理系统设计

    Institute of Scientific and Technical Information of China (English)

    邹金松

    2015-01-01

    The monitoring and management system construction of the anchorage at the upstream of the Three Gorges Dam Lock can raise the efficiencies of shipping lockage capacity and give full play to the benefits of waterway transportation.The current status of the safety supervision in the Three Gorges Reservoir Region is analyzed for the framework designing of the target system.Furthermore, we can design the monitoring and management system framework of the anchorage at the upstream of the Three Gorges Dam Lock and the main function modules according to the analysis of system re-quirements.This system includes four functional sub-systems,namely,monitoring data collection sub-system,navigational environment information release sub-system,anchorage status dynamic mo-nitoring sub-system,and anchorage business management sub-system.The designed system can assist in supervising the waterways,ship locks,docks and ferries in the Three Gorges Reservoir Region.%三峡船闸上游锚地监控管理系统的建设可以帮助提高船舶过闸效率,充分发挥三峡工程的通航效益。对三峡坝区的安全监管现状进行分析,设计了三峡船闸上游锚地监控管理系统的架构,根据系统主体需求对主要功能模块进行设计。该系统主要包括监控数据采集、通航环境信息发布、锚地状态动态监控和锚地业务管理等子系统。该系统可实现对三峡库区航道、船闸、码头和渡口等重点水域的监控。

  18. Orthodontic retreatment using anchorage with miniplate to camouflage a Class III skeletal pattern.

    Science.gov (United States)

    Farret, Marcel Marchiori

    2016-06-01

    This manuscript describes the treatment of a 27-year-old patient who was previously treated with two maxillary first premolar extractions. The patient had skeletal Class III malocclusion, Class III canine relationship, anterior crossbite, and a concave profile. As the patient refused orthognathic surgery, a miniplate was used on the right side of the lower arch as an anchorage unit after the extraction of mandibular first premolars, aiding the retraction of anterior teeth. At the end of treatment, anterior crossbite was corrected, in which first molars and canines were in a Class I relationship, and an excellent intercuspation was reached. Furthermore, patient's profile remarkably improved as a result of mandibular incisor retraction. A 30-month follow-up showed good stability of the results obtained. This case was presented to the Brazilian Board of Orthodontics and Dentofacial Orthopedics (BBO) as one of the requirements to become diplomate by the BBO. PMID:27409659

  19. Denmark: criminal law as an anchorage point for pro-active anti-terrorism legislation

    DEFF Research Database (Denmark)

    Vestergaard, Jørn

    2012-01-01

    The article focuses on the compatibility of criminal law anti-terrorism legislation in Danish law with basic principles regarding the rule of law and due process. The so-called anti-terror-packages of 2002 and 2006 involve a set of rather uncertain and wide-reaching provisions fundamentally...... instruments binding on Denmark by virtue of EU law or international obligations. And the statutes have been drafted in a somewhat loose manner without sufficiently thorough legislative preparations. The provisions in the Penal Code constitute an anchorage point for all other anti-terrorism legislation. Thus......, the challenges regarding rule of law and due process also relate to secondary legislation in fields like law enforcement, criminal procedure, intelligence, public law, treatment of foreigners, etc....

  20. Anchorage Test of A New-style Water-fast Anchorage Agent and Engineering Application%新型耐水锚固剂锚固试验研究及工程应用

    Institute of Scientific and Technical Information of China (English)

    王正胜

    2015-01-01

    Selectinghigh-strength modified polyester which could trigger solidification in water environment, water-fast anchorage agent was developed successfully.Research showed that action system of PM-Ⅰ and solidification agent-Ⅰ made solidification effect be the best and anchorage strength the biggest under normal temperature environment, and that pulling resistance could reach above 240kN after 24 hours under anhydrous condition, it could reach above 200kN under water condition.In simulated borehole with seamless steel tube with 28mm inner diameter and 800mm length, anchorage strength of 2 self-made 2330 anchorage agents reached 273kN.This new-style water-fast anchorage agent was successfully applied in floor-heave main air-return roadway of 2nd mining region in Jiangjiahe Col-liery, which improved construction efficiency, shortened construction time and reduced the influence of water on anchorage force of floor anchored cable.%选取本身强度高,水域环境内能稳定引发固化的改性聚酯为主体,研制成功耐水锚固剂。研究表明: PM-Ⅰ和固化剂-Ⅰ反应体系,常温固化效果最好、锚固强度最高;无水条件下24h后抗拔力达到240kN以上,有水条件下24h后抗拔力达到200kN以上;内径28mm、长800mm无缝钢管模拟钻孔中,自制2330锚固剂2支,锚固锚索24h后锚固强度可达273kN。新型耐水锚固剂于蒋家河煤矿二号采区回风大巷底鼓段试验成功,且提高施工效率、缩短工期,减少了水对底板锚索锚固力降低的影响。

  1. Correlação entre instrumentos de qualidade de vida relacionada à saúde e independência funcional em idosos com insuficiência cardíaca Correlation between instruments for measuring health-related quality of life and functional independence in elderly with heart failure

    Directory of Open Access Journals (Sweden)

    Fátima Ayres de Araújo Scattolin

    2007-11-01

    Full Text Available Este estudo teve como objetivo identificar a correlação entre as medidas de qualidade de vida relacionada à saúde e independência funcional em idosos com insuficiência cardíaca. Fizeram parte do estudo 146 idosos com insuficiência cardíaca em tratamento ambulatorial, que responderam ao Minnesota Living With Heart Failure Questionnaire (LHFQ e à Medida de Independência Funcional (MIF. Foi utilizado o coeficiente de correlação de Spearman para verificar a correlação entre as medidas de qualidade de vida relacionada à saúde e MIF, e entre estas e a Classificação Funcional da New York Heart Association (CF-NYHA. Foram constatadas correlações significantes de moderada magnitude entre as dimensões física e emocional do LHFQ e as subescalas total e motora da MIF. Os resultados evidenciaram que a qualidade de vida relacionada à saúde e independência funcional são conceitos correlacionados e influenciados pela CF-NYHA. Os resultados sugerem que ações voltadas à promoção da independência funcional podem otimizar a qualidade de vida relacionada à saúde de idosos com insuficiência cardíaca, especialmente na dimensão física. São necessários novos estudos com ampliação da amostra, inclusão de pacientes de faixa etária mais elevada e com maior gravidade da doença, para verificar a reprodutibilidade destes achados.The main purpose of this study was to identify the correlation between health-related quality of life and the Functional Independence Measure (FIM in elderly patients with heart failure. 146 elderly outpatients completed the Minnesota Living with Heart Failure Questionnaire (LHFQ and the FIM. Spearman correlation coefficient was used to identify correlations between health-related quality of life and FIM, and between them and New York Heart Association (NYHA class. Statistically significant moderate correlations were found between physical and emotional LHFQ and total FIM and motor subscale. The results

  2. Independence in appearance

    DEFF Research Database (Denmark)

    Warming-Rasmussen, Bent; Quick, Reiner; Liempd, Dennis van

    2011-01-01

    article presents research contributions to the question whether the auditor is to continue to provide both audit and non-audit services (NAS) to an audit client. Research results show that this double function for the same audit client is a problem for stakeholders' confidence in auditor independence....... The green Paper proposes that a solution could be to prohibit NAS to audit clients. Research results indicate alternative possibilitiers: e.g. banning the most harmful non-audit services and / or limiting the relative share of fees from NAS...

  3. Decoding the Encoding of Functional Brain Networks: an fMRI Classification Comparison of Non-negative Matrix Factorization (NMF), Independent Component Analysis (ICA), and Sparse Coding Algorithms

    OpenAIRE

    Xie, Jianwen; Douglas, Pamela K.; Wu, Ying Nian; Brody, Arthur L.; Anderson, Ariana E.

    2016-01-01

    Brain networks in fMRI are typically identified using spatial independent component analysis (ICA), yet mathematical constraints such as sparse coding and positivity both provide alternate biologically-plausible frameworks for generating brain networks. Non-negative Matrix Factorization (NMF) would suppress negative BOLD signal by enforcing positivity. Spatial sparse coding algorithms ($L1$ Regularized Learning and K-SVD) would impose local specialization and a discouragement of multitasking,...

  4. Change in body fat mass is independently associated with executive functions in older women: a secondary analysis of a 12-month randomized controlled trial.

    Directory of Open Access Journals (Sweden)

    Elizabeth Dao

    Full Text Available OBJECTIVES: To investigate the independent contribution of change in sub-total body fat and lean mass to cognitive performance, specifically the executive processes of selective attention and conflict resolution, in community-dwelling older women. METHODS: This secondary analysis included 114 women aged 65 to 75 years old. Participants were randomly allocated to once-weekly resistance training, twice-weekly resistance training, or twice-weekly balance and tone training. The primary outcome measure was the executive processes of selective attention and conflict resolution as assessed by the Stroop Test. Sub-total body fat and lean mass were measured by dual-energy x-ray absorptiometry (DXA to determine the independent association of change in both sub-total body fat and sub-total body lean mass with Stroop Test performance at trial completion. RESULTS: A multiple linear regression model showed reductions in sub-total body fat mass to be independently associated with better performance on the Stroop Test at trial completion after accounting for baseline Stroop performance, age, baseline global cognitive state, baseline number of comorbidities, baseline depression, and experimental group. The total variance explained was 39.5%; change in sub-total body fat mass explained 3.9% of the variance. Change in sub-total body lean mass was not independently associated with Stroop Test performance (P>0.05. CONCLUSION: Our findings suggest that reductions in sub-total body fat mass - not sub-total lean mass - is associated with better performance of selective attention and conflict resolution.

  5. Bio-cultural anchorage of the prickly pear cactus in Tlalnepantla (Morelos, Mexico

    Directory of Open Access Journals (Sweden)

    Torres-Salcido, Gerardo

    2016-06-01

    Full Text Available The prickly pear cactus is a source of food with strong bio-cultural anchorage in Mexico. This is due to at least three factors: 1 the nature and heritage of cacti; 2 cultural heritage; and 3 the socio-cultural relationships with historical and symbolic roots that have facilitated knowledge of how to cultivate it and how to use it. The aim of this article is to put factors of territorial anchorage and its historical transformation in context by examining the case of the municipality of Tlalnepantla in the state of Morelos, Mexico. This community has experienced accelerated change due to the exchange of traditional crops for the prickly pear cactus and the integration of farming, commercialization and agro-transformation. Our hypothesis is that the market, internal conflicts and a lack of socio-institutional coordination have put social organization into crisis, favoring the territorial spread of the prickly pear cactus and making the Local Agro-Food Systems (LAFS of Tlalnepantla less competitive. The conclusions highlight important economic and social advances whose roots lie in the strengthening and anchorage of the territory-product. However, circumstances both internal and external to the community persist, such as intra-community conflicts, the international market and cultural paradigm shifts that affect the producers and put consolidation of the LAFS at risk.El nopal es un alimento con un fuerte anclaje bio-cultural en México, propiciado por al menos tres factores: 1 la naturaleza y el patrimonio de cactáceas; 2 el patrimonio cultural; y, 3 las relaciones socio-culturales que han permitido un “saber hacer” y un “saber utilizar” con raíces históricas y simbólicas. El objetivo es situar los factores de anclaje territorial y su transformación histórica tomando como caso el municipio de Tlalnepantla, en el estado de Morelos, México. Esta comunidad ha experimentado un acelerado cambio por la reconversión de los cultivos

  6. RalGPS2 Is Essential for Survival and Cell Cycle Progression of Lung Cancer Cells Independently of Its Established Substrates Ral GTPases.

    Directory of Open Access Journals (Sweden)

    Adriana O Santos

    Full Text Available The human genome contains six genes coding for proteins validated in vitro as specific activators of the small GTPases "Ras-related protein Ral-A" and "Ras-related protein Ral-B", generically named Ral-guanine nucleotide exchange factors (RalGEF. Ral proteins are important contributors to Ras oncogenic signaling, and RAS oncogenes are important in human Non-Small Cell Lung Carcinoma (NSCLC. Therefore in this work, RalGEF contribution to oncogenic and non-oncogenic features of human NSCLC cell lines, as anchorage-dependent and independent growth, cell survival, and proliferation, was investigated. Among all human RalGEF, silencing of RGL1 and RALGPS1 had no detectable effect. However, silencing of either RGL2, RGL3, RALGDS or, to a larger extent, RALGPS2 inhibited cell population growth in anchorage dependent and independent conditions (up to 90 and 80%, respectively. RALGPS2 silencing also caused an increase in the number of apoptotic cells, up to 45% of the cell population in transformed bronchial BZR cells. In H1299 and A549, two NSCLC cell lines, RALGPS2 silencing caused an arrest of cells in the G0/G1-phase of cell cycle. Furthermore, it was associated with the modulation of important cell cycle regulators: the E3 Ubiquitin Protein Ligase S-phase kinase-associated protein 2 (Skp2 was strongly down-regulated (both at mRNA and protein levels, and its targets, the cell cycle inhibitors p27 and p21, were up-regulated. These molecular effects were not mimicked by silencing RALA, RALB, or both. However, RALB silencing caused a modest inhibition of cell cycle progression, which in H1299 cells was associated with Cyclin D1 regulation. In conclusion, RALGPS2 is implicated in the control of cell cycle progression and survival in the in vitro growth of NSCLC cell lines. This function is largely independent of Ral GTPases and associated with modulation of Skp2, p27 and p21 cell cycle regulators.

  7. Consecutive condylectomy and molar intrusion using temporary anchorage devices as an alternative for correcting facial asymmetry with condylar hyperplasia.

    Science.gov (United States)

    Choi, Yoon Jeong; Lee, Sang-Hwy; Baek, Man-Suk; Kim, Jae-Young; Park, Young-Chel

    2015-04-01

    This case report demonstrates the successful treatment of facial asymmetry with condylar hyperplasia with limited surgical and orthodontic treatment. A high condylectomy was performed to shorten the elongated condyle and to remove its active growth site. The maxillary molars on the affected side were then orthodontically intruded using temporary anchorage devices to improve the occlusal cant and posterior open bite of the unaffected side. This combined surgical-orthodontic treatment provided a satisfactory outcome without additional orthognathic surgery. PMID:25836342

  8. Orthodontic anchoring techniques and its influence on pain, discomfort, and jaw function--a randomized controlled trial.

    Science.gov (United States)

    Feldmann, Ingalill; List, Thomas; Bondemark, Lars

    2012-02-01

    The aim of this trial was to evaluate and compare perceived pain, discomfort, and jaw function impairment between orthodontic treatments combined with skeletal anchorage and treatment using conventional anchorage with headgear or transpalatal bar. A total of 120 adolescent patients in order to start orthodontic treatment were consecutively recruited and randomized into three groups with different anchorage. Group A underwent installation of a skeletal anchorage (Onplant or Orthosystem implant), group B received headgear, and group C a transpalatal bar. Questionnaires were used to assess pain intensity, discomfort, analgesic consumption, and jaw function impairment from baseline to the end of treatment. Pain scores overall peaked on day 2 and were almost back to baseline on day 7. The site with the highest pain scores during treatment was incisors in contact but with no differences between groups. Pain intensity from molars was significantly less in the skeletal anchorage group A compared to the transpalatal bar group C the first 4 days in treatment and with no sign differences compared to headgear. The results confirm that there were very few significant differences between patients' perceptions of skeletal and conventional anchorage systems during orthodontic treatment. Consequently, these new appliances were well accepted by the patients in a long time perspective and can thus be recommended.

  9. Combining the independent pixel and point-spread function approaches to simulate the actinic radiation field in moderately inhomogeneous 3D cloudy media

    International Nuclear Information System (INIS)

    A fast method is presented for gaining 3D actinic flux density fields, Fact, in clouds employing the Independent Pixel Approximation (IPA) with a parameterized horizontal photon transport to imitate radiative smoothing effects. For 3D clouds the IPA is an efficient method to simulate radiative transfer, but it suffers from the neglect of horizontal photon fluxes leading to significant errors (up to locally 30% in the present study). Consequently, the resulting actinic flux density fields exhibit an unrealistically rough and rugged structure. In this study, the radiative smoothing is approximated by applying a physically based smoothing algorithm to the calculated IPA actinic flux field.

  10. Long-term adherence to exercise: the relationship with functional fitness and personal motivation among community-dwelling independent-living older women

    Directory of Open Access Journals (Sweden)

    Philipe de Souto BarretoI

    2011-03-01

    Full Text Available OBJECTIVE: The aim of this study were to examine the relationships between functional fitness, self-esteem, aesthetic body care and long-term adherence to dance, and to identify other characteristics related to dance adherence in the elderly. METHODS: Seventeen women, aged 59-86 years-old, who practised a type of dance labeled "bodily expression". RESULTS: Functional fitness was correlated to dance adherence and was the only factor related to this variable in a linear regression analysis. Self-esteem and aesthetic body care seemed to play an indirect role on adherence. Functional fitness, specially upper-body flexibility and lower-body muscular function, and interest in dance (qualitative data were related to long-term adherence to dance. CONCLUSIONS: Functional fitness and interest in exercising were the main aspects related to participants' long-term adherence to dance.

  11. Liquid marbles for high-throughput biological screening of anchorage-dependent cells.

    Science.gov (United States)

    Oliveira, Nuno M; Correia, Clara R; Reis, Rui L; Mano, João F

    2015-01-28

    Stable liquid marbles (LM) are produced by coating liquid droplets with a hydrophobic powder. The used hydrophobic powder is produced by fluorosi-lanization of diatomaceous earth, used before to produce superhydrophobic structures. Here, the use of LM is proposed for high-throughput drug screening on anchorage-dependent cells. To provide the required cell adhesion sites inside the liquid environment of LM, surface-modified poly(l-lactic acid) microparticles are used. A simple method that takes advantage from LM appealing features is presented, such as the ability to inject liquid on LM without disrupting (self-healing ability), and to monitor color changes inside of LM. After promoting cell adhesion, a cytotoxic screening test is performed as a proof of concept. Fe(3+) is used as a model cytotoxic agent and is injected on LM. After incubation, AlamarBlue reagent is injected and used to assess the presence of viable cells, by monitoring color change from blue to red. Color intensity is measured by image processing and the analysis of pictures takes using an ordinary digital camera. The proposed method is fully validated in counterpoint to an MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carbo​xymethoxyphenyl)-2-(4-sulfophenyl)-2H-te​trazolium) colorimetric assay, a well-known method used for the cytotoxicity assessment.

  12. Oral and dental restoration of wide alveolar cleft using distraction osteogenesis and temporary anchorage devices.

    Science.gov (United States)

    Rachmiel, Adi; Emodi, Omri; Gutmacher, Zvi; Blumenfeld, Israel; Aizenbud, Dror

    2013-12-01

    Closure of large alveolar clefts and restoration by a fixed bridge supported by implants is a challenge in cleft alveolus treatment. A major aesthetic concern with distraction osteogenesis is obtaining a predictable position of the implant in relation to the newly generated bony alveolar ridge. We describe the treatment of a large cleft alveolus and palate reconstruction by distraction osteogenesis utilizing temporary anchorage devices (TADs) followed by a fixed implant-supported bridge. The method consists of segmental bone transport by distraction osteogenesis using a bone-borne distractor to minimize the alveolar cleft, followed by closure of the residual small defect by bone grafting three months later. During the active transport distraction, TADs were used exerting multidirectional forces to control the distraction vector forward and laterally for better interarch relation. A vertical alveolar distraction of the newly reconstructed bone of 15 mm facilitated optimal implant placement. The endosseous implants were osteointegrated and supported a fixed dental prosthesis. In conclusion, the large cleft alveolus defect was repaired in three dimensions by distraction osteogenesis assisted by TADs, and the soft tissues expanded simultaneously. Endosseous implants were introduced in the newly reconstructed bone for a fixed dental prosthesis enabling, rehabilitation of aesthetics, eating and speaking.

  13. The perception of pain following interdental microimplant treatment for skeletal anchorage: a retrospective study.

    Science.gov (United States)

    Chen, Chun-Ming; Chang, Chao-San; Tseng, Yu-Chuan; Hsu, Kun-Rong; Lee, Kun-Tsung; Lee, Huey-Er

    2011-01-01

    During orthodontic therapy, patients frequently complain about pain and discomfort, especially during insertion of fixed appliances. Skeletal anchorage using an interdental microimplant is a new concept in orthodontic treatment. The purpose of this study was to investigate differences and changes in the level of pain among patients in relation to orthodontic microimplant treatments. Forty microimplants were applied to the maxilla as skeletal anchors in the orthodontic treatment. The visual analog scale (VAS) was used to evaluate the patients' perception of pain during this new modality treatment. The premolar extraction VAS core was used as a baseline for the complete orthodontic procedure. The mean VAS score was 35.8 mm at 24 h after premolar extraction. The mean VAS score for insertion and removal of the microimplant 24 h after the operation was 12.3 and 7.8 mm, respectively. Three months after removal of the skeletal anchors, the VAS score had decreased to 3.2 mm and was the same as with the traditional orthodontic treatment. By using the repeated-measure general linear model (GLM), we found that the score 1 day after microimplant placement was significantly less than that 1 day after first premolar extraction or that 1 day after fixed appliance insertion. This result indicates that interdental microimplant did not generate any greater pain than other orthodontic procedures. Therefore, patients were willing to adopt the new orthodontic treatment.

  14. Research on the video detection device in the invisible part of stay cable anchorage system

    Science.gov (United States)

    Cai, Lin; Deng, Nianchun; Xiao, Zexin

    2012-11-01

    The cables in anchorage zone of cable-stayed bridge are hidden within the embedded pipe, which leads to the difficulty for detecting the damage of the cables with visual inspection. We have built a detection device based on high-resolution video capture, realized the distance observing of invisible segment of stay cable and damage detection of outer surface of cable in the small volume. The system mainly consists of optical stents and precision mechanical support device, optical imaging system, lighting source, drived motor control and IP camera video capture system. The principal innovations of the device are ⑴A set of telescope objectives with three different focal lengths are designed and used in different distances of the monitors by means of converter. ⑵Lens system is far separated with lighting system, so that the imaging optical path could effectively avoid the harsh environment which would be in the invisible part of cables. The practice shows that the device not only can collect the clear surveillance video images of outer surface of cable effectively, but also has a broad application prospect in security warning of prestressed structures.

  15. Structure-function analysis of nucleolin and ErbB receptors interactions.

    Directory of Open Access Journals (Sweden)

    Keren Farin

    Full Text Available BACKGROUND: The ErbB receptor tyrosine kinases and nucleolin are major contributors to malignant transformation. Recently we have found that cell-surface ErbB receptors interact with nucleolin via their cytoplasmic tail. Overexpression of ErbB1 and nucleolin leads to receptor phosphorylation, dimerization and anchorage independent growth. METHODOLOGY/PRINCIPAL FINDINGS: In the present study we explored the regions of nucleolin and ErbB responsible for their interaction. Using mutational analyses, we addressed the structure-function relationship of the interaction between ErbB1 and nucleolin. We identified the ErbB1 nuclear localization domain as nucleolin interacting region. This region is important for nucleolin-associated receptor activation. Notably, though the tyrosine kinase domain is important for nucleolin-associated receptor activation, it is not involved in nucleolin/ErbB interactions. In addition, we demonstrated that the 212 c-terminal portion of nucleolin is imperative for the interaction with ErbB1 and ErbB4. This region of nucleolin is sufficient to induce ErbB1 dimerization, phosphorylation and growth in soft agar. CONCLUSIONS/SIGNIFICANCE: The oncogenic potential of ErbB depends on receptor levels and activation. Nucleolin affects ErbB dimerization and activation leading to enhanced cell growth. The C-terminal region of nucleolin and the ErbB1 NLS-domain mediate this interaction. Moreover, when the C-terminal 212 amino acids region of nucleolin is expressed with ErbB1, it can enhance anchorage independent cell growth. Taken together these results offer new insight into the role of ErbB1 and nucleolin interaction in malignant cells.

  16. Independence and Product Systems

    OpenAIRE

    Skeide, Michael

    2003-01-01

    Starting from elementary considerations about independence and Markov processes in classical probability we arrive at the new concept of conditional monotone independence (or operator-valued monotone independence). With the help of product systems of Hilbert modules we show that monotone conditional independence arises naturally in dilation theory.

  17. Left ventricular dyssynchrony assessed by gated SPECT phase analysis is an independent predictor of death in patients with advanced coronary artery disease and reduced left ventricular function not undergoing cardiac resynchronization therapy

    Energy Technology Data Exchange (ETDEWEB)

    Uebleis, Christopher; Hellweger, Stefan; Lehner, Sebastian; Haug, Alexander; Bartenstein, Peter; Cumming, Paul; Hacker, Marcus [Ludwig-Maximilians University, Department of Nuclear Medicine, Munich (Germany); Laubender, Ruediger Paul [Ludwig-Maximilians University, Institute of Medical Informatics, Biometry, and Epidemiology (IBE), Munich (Germany); Becker, Alexander [Ludwig-Maximilians University, Medical Department I, Munich (Germany); Sohn, Hae-Young [Ludwig-Maximilians University, Medical Department Innenstadt, Munich (Germany); Van Kriekinge, Serge D.; Slomka, Piotr J. [Cedars-Sinai Medical Center, Los Angeles, CA (United States); UCLA, David Geffen School of Medicine, Los Angeles, CA (United States)

    2012-10-15

    Left ventricular (LV) mechanical dyssynchrony (LVMD) was assessed by gated single-photon emission CT myocardial perfusion imaging (MPI) as an independent predictor of death from any cause in patients with known coronary artery disease (CAD) and reduced LV function. Between 2001 and 2010, 135 patients (64 {+-} 11 years of age, 84 % men) with known CAD, reduced LV ejection fraction (LVEF, 38 {+-} 15 %) and without an implanted cardiac resynchronization therapy device underwent gated MPI at rest. LV functional evaluation, which included phase analysis, was conducted to identify patients with LVMD. Kaplan-Meier survival curves were calculated for death of any cause during a mean follow-up of 2.0 {+-} 1.7 years. Uni- and multivariate Cox proportional hazards regression models were calculated to identify independent predictors of death from any cause. Of the 135 patients, 30 (22 %) died during follow-up (18 cardiac deaths and 12 deaths from other causes). Kaplan-Meier curves showed a significantly shorter survival time in the patients with severely reduced LVEF (<30 %, n = 45) or with LVMD (n = 81, log-rank test P <0.005). Cox models identified LVMD, LVEF <30 % and a total perfusion deficit at rest of {>=}20 % as independent predictors of death from any cause. While patients with LVEF <30 % in conjunction with LVMD had similar survival times irrespective of whether they had early revascularization or medical therapy, those patients with LVEF {>=}30% and LVMD who underwent revascularization had significantly longer survival. In patients with known CAD and reduced LV function, dyssynchrony of the LV is an independent predictor of death from any cause. (orig.)

  18. Left ventricular dyssynchrony assessed by gated SPECT phase analysis is an independent predictor of death in patients with advanced coronary artery disease and reduced left ventricular function not undergoing cardiac resynchronization therapy

    International Nuclear Information System (INIS)

    Left ventricular (LV) mechanical dyssynchrony (LVMD) was assessed by gated single-photon emission CT myocardial perfusion imaging (MPI) as an independent predictor of death from any cause in patients with known coronary artery disease (CAD) and reduced LV function. Between 2001 and 2010, 135 patients (64 ± 11 years of age, 84 % men) with known CAD, reduced LV ejection fraction (LVEF, 38 ± 15 %) and without an implanted cardiac resynchronization therapy device underwent gated MPI at rest. LV functional evaluation, which included phase analysis, was conducted to identify patients with LVMD. Kaplan-Meier survival curves were calculated for death of any cause during a mean follow-up of 2.0 ± 1.7 years. Uni- and multivariate Cox proportional hazards regression models were calculated to identify independent predictors of death from any cause. Of the 135 patients, 30 (22 %) died during follow-up (18 cardiac deaths and 12 deaths from other causes). Kaplan-Meier curves showed a significantly shorter survival time in the patients with severely reduced LVEF (<30 %, n = 45) or with LVMD (n = 81, log-rank test P <0.005). Cox models identified LVMD, LVEF <30 % and a total perfusion deficit at rest of ≥20 % as independent predictors of death from any cause. While patients with LVEF <30 % in conjunction with LVMD had similar survival times irrespective of whether they had early revascularization or medical therapy, those patients with LVEF ≥30% and LVMD who underwent revascularization had significantly longer survival. In patients with known CAD and reduced LV function, dyssynchrony of the LV is an independent predictor of death from any cause. (orig.)

  19. 关于支抗和支抗力科学内涵的辨析%Scientific connotation analysis on anchorage and resistance

    Institute of Scientific and Technical Information of China (English)

    陈勃

    2011-01-01

    目的 明确口腔正畸学标志性术语一支抗和支抗力的科学内涵.方法 查阅历史相关文献并剖析其对支抗和支抗力定义的不科学性.结果 现有文献资料未能对支抗和支抗力作出科学的定义.结论 支抗和支抗力是口腔正畸学的标志性术语,明确其科学内涵有重要的理论价值和实践意义.它可以帮助提高临床正畸医师进行有效支抗控制的主动意识,并为各种外加的支抗加强装置提供科学的理论依据,可以说没有支抗和支抗力指导的有效支抗控制就不可能有理想的正畸治疗效果.%Objective To clear the symbolic term in Orthodontics - anchorage and scientific connotation of resistance.Methods Searched history documents and dissected its unscientific definition to anchorage and resistance.Results Current document literature failed to give the scientific definition towards anchorage and resistance.Conclusion Anchorage and resistance are symbolic terms in orthodontics.Clarifying its scientific connotation has important theoretical value and practical significance.It can help orthodontist enhance active consciousness on controlling anchorage effectively.Besides,it can provide scientific theoretical basis for different kinds of additional anchorage strengthening devices.It can be said without the guidance of effective resistance anchorage control.We can say there won't be ideal orthodontic treatment.

  20. Research Progress of the Anchorage Control in Orthodontic Treatment%正畸治疗中支抗控制的研究进展

    Institute of Scientific and Technical Information of China (English)

    刘春利; 肖丹娜

    2015-01-01

    正畸治疗中支抗控制至关重要,合理的支抗设计关系到治疗的成败。支抗控制可从支抗加强和支抗节省两方面考虑,并在治疗过程中联合应用。正畸治疗效果需以多维效果评价,为取得优质的治疗效果支抗需根据患者的垂直骨面型、牙颌畸形的类型,在矢状向、垂直向、水平向进行控制。该文就目前常用的加强支抗的方法进行简要概括,同时对新兴加强支抗的设计进行简要总结,并对支抗节省的理念、操作和矫治系统的选择进行综述。%The anchorage control is very important in orthodontic treatment,and good design matters to the success or failure of the treatment.Anchorage control includes two aspects of anchorage strengthening and anchorage saving,which both should be taken into consideration,and joint applied in the process of treat-ment.Effect of orthodontic treatment needs multidimensional performance evaluation,to achieve high quality of treatment,sagittal,vertical,and horizontal control should be done according to patients'vertical bone sur-face type and the type of teeth micromaxillary deformity.Here is to make a review of the common methods to strengthen the anchorage,as well as the emerging of strengthening anchorage design,and the idea of the anchorage saving,and the choices of operational and correctional systems.

  1. Powerful Independent Directors

    OpenAIRE

    Kathy Fogel; Liping Ma; Randall Morck

    2014-01-01

    Shareholder valuations are economically and statistically positively correlated with independent directors’ power, gauged by social network power centrality. Powerful independent directors’ sudden deaths reduce shareholder value significantly; other independent directors’ deaths do not. More powerful independent directors Granger cause higher valuations; the converse is not true. Further tests associate more powerful independent directors with less value-destroying M&A, less free cash flow re...

  2. Central Bank independence

    OpenAIRE

    Vasile DEDU; Tiberiu STOICA

    2012-01-01

    In this paper we present the key aspects regarding central bank’s independence. Most economists consider that the factor which positively influences the efficiency of monetary policy measures is the high independence of the central bank. We determined that the National Bank of Romania (NBR) has a high degree of independence. NBR has both goal and instrument independence. We also consider that the hike of NBR’s independence played an important role in the significant disin...

  3. Introduction to algebraic independence theory

    CERN Document Server

    Philippon, Patrice

    2001-01-01

    In the last five years there has been very significant progress in the development of transcendence theory. A new approach to the arithmetic properties of values of modular forms and theta-functions was found. The solution of the Mahler-Manin problem on values of modular function j(tau) and algebraic independence of numbers pi and e^(pi) are most impressive results of this breakthrough. The book presents these and other results on algebraic independence of numbers and further, a detailed exposition of methods created in last the 25 years, during which commutative algebra and algebraic geometry exerted strong catalytic influence on the development of the subject.

  4. ACE inhibition with spirapril improves diastolic function at rest independent of vasodilation during treatment with spirapril in mild to moderate hypertension

    DEFF Research Database (Denmark)

    Petersen, J R; Drabaek, H; Gleerup, Christian Peter-Ole G.;

    1996-01-01

    The effects of the ACE inhibitor spirapril and of hydrochlorothiazide on left ventricular diastolic function were studied. Thirteen patients with mild to moderate essential hypertension completed this randomized, double-blinded, placebo-controlled, crossover study. After a three-week run-in period...... indicate that spirapril lowers A/E ratio within four weeks in patients with mild to moderate essential hypertension. It thereby seems able to improve left ventricular diastolic function. The effect is not dependent upon changes in hemodynamic variables, blood pressure, left ventricular mass, or end...

  5. Elevation of IL-6 in the allergic asthmatic airway is independent of inflammation but associates with loss of central airway function

    Directory of Open Access Journals (Sweden)

    Bunn Janice Y

    2010-03-01

    Full Text Available Abstract Background Asthma is a chronic inflammatory disease of the airway that is characterized by a Th2-type of immune response with increasing evidence for involvement of Th17 cells. The role of IL-6 in promoting effector T cell subsets suggest that IL-6 may play a functional role in asthma. Classically IL-6 has been viewed as an inflammatory marker, along with TNFα and IL-1β, rather than as regulatory cytokine. Objective To investigate the potential relationship between IL-6 and other proinflammatory cytokines, Th2/Th17 cytokines and lung function in allergic asthma, and thus evaluate the potential role of IL-6 in this disease. Methods Cytokine levels in induced sputum and lung function were measured in 16 healthy control and 18 mild-moderate allergic asthmatic subjects. Results The levels of the proinflammatory biomarkers TNFα and IL-1β were not different between the control and asthmatic group. In contrast, IL-6 levels were specifically elevated in asthmatic subjects compared with healthy controls (p S = 0.53, p Conclusions In mild-moderate asthma, IL-6 dissociates from other proinflammatory biomarkers, but correlates with IL-13 levels. Furthermore, IL-6 may contribute to impaired lung function in allergic asthma.

  6. Frame independent cosmological perturbations

    Energy Technology Data Exchange (ETDEWEB)

    Prokopec, Tomislav; Weenink, Jan, E-mail: t.prokopec@uu.nl, E-mail: j.g.weenink@uu.nl [Institute for Theoretical Physics and Spinoza Institute, Utrecht University, Leuvenlaan 4, 3585 CE Utrecht (Netherlands)

    2013-09-01

    We compute the third order gauge invariant action for scalar-graviton interactions in the Jordan frame. We demonstrate that the gauge invariant action for scalar and tensor perturbations on one physical hypersurface only differs from that on another physical hypersurface via terms proportional to the equation of motion and boundary terms, such that the evolution of non-Gaussianity may be called unique. Moreover, we demonstrate that the gauge invariant curvature perturbation and graviton on uniform field hypersurfaces in the Jordan frame are equal to their counterparts in the Einstein frame. These frame independent perturbations are therefore particularly useful in relating results in different frames at the perturbative level. On the other hand, the field perturbation and graviton on uniform curvature hypersurfaces in the Jordan and Einstein frame are non-linearly related, as are their corresponding actions and n-point functions.

  7. Mini-placa como ancoragem ortodôntica: relato de caso Mini screw as orthodontic anchorage: case report

    Directory of Open Access Journals (Sweden)

    André Luiz Zétola

    2005-08-01

    ígida pode ser utilizada com bastante propriedade no tratamento ortodôntico qunado for requerida uma ancoragem máxima.It is needless to say that anchorage control is necessary to a successful orthodontic treatment. Without it, it would be impossible to obtain an ideal occlusion through orthodontic mechanics in the majority of cases. The utilization of rigid devices, originated from face traumatology and implant dentistry principles, to obtain rigid anchorage in orthodontics revolutionized the way of treating cases with an unsatisfactory prognosis. Specially in cases where a lot of anchorage is needed and it is difficult or impossible to get it. The objective of this article was to revise the literature related to the use of mini-screw implant systems, to report a case where it was utilized and to discuss some aspects related to the usage of rigid anchorage orthodontics. The rigid anchorage was used in a 39 year old female patient, who had the upper left molars with a relevant extrusion due to the absence of the lower left molars. Fixed appliances were placed on the upper arch with a TPA connecting the second bicuspids. The TPA was used to anchor the intrusion of the upper left molars. A quad-helix appliance was used to correct the upper right third molar crossbite. Since the orthodontic intrusion with only dental anchorage did not have a satisfactory result, an "L" shaped titanium plate was placed on the maxilla, at the apical region of the upper first and second left molars, with the purpose of obtaining a rigid orthodontic anchorage to intrude them. Elastics were used during five months with intrusive force,the obtained intrusion was 6 millimeters. After the removal of the appliance, upper and lower dental bleaching was made and final prosthesis were placed over the implants. Through the literature reviewed and the case reported, it can be concluded that rigid anchorage can be indicated in orthodontic treatments where there is lack of anchorage or the need of an absolute

  8. Short-axis epicardial volume change is a measure of cardiac left ventricular short-axis function, which is independent of myocardial wall thickness.

    Science.gov (United States)

    Ugander, Martin; Carlsson, Marcus; Arheden, Håkan

    2010-02-01

    Fractional shortening (FS) by echocardiography is considered to represent the short-axis contribution to the stroke volume (SV), also called short-axis function. However, FS is mathematically coupled to the amount of myocardium, since it rearranges during atrioventricular plane displacement (AVPD). The SV is the sum of the volumes generated by 1) reduction in outer volume of the heart, and 2) inner AVPD. The long-axis contribution to the SV is generated by AVPD, and thus the short-axis contribution is the remaining outer volume change of the heart, which should be unrelated to myocardial wall thickness. We hypothesized that both endocardial and midwall shortening indexed to SV are dependent on myocardial wall thickness, whereas epicardial volume change (EVC) indexed to SV is not. Twelve healthy volunteers (normals), 12 athletes, and 12 patients with dilated cardiomyopathy (ejection fraction EVC was defined as SV minus long-axis function. Endocardial and midwall shortening were measured in a midventricular short-axis slice. Endocardial shortening/SV and midwall shortening/SV both varied in relation to end-diastolic myocardial wall thickness (R(2) = 0.16, P = 0.008 and R(2) = 0.14, P = 0.012, respectively), whereas EVC/SV did not (R(2) = 0.00, P = 0.37). FS is dependent on myocardial wall thickness, whereas EVC is not and therefore represents true short-axis function. This is not surprising considering that FS is mainly caused by rearrangement of myocardium secondary to long-axis function. FS is therefore not synonymous with short-axis function.

  9. Identification and functional characterization of NifA variants that are independent of GlnB activation in the photosynthetic bacterium Rhodospirillum rubrum.

    Science.gov (United States)

    Zou, Xiaoxiao; Zhu, Yu; Pohlmann, Edward L; Li, Jilun; Zhang, Yaoping; Roberts, Gary P

    2008-09-01

    The activity of NifA, the transcriptional activator of the nitrogen fixation (nif) gene, is tightly regulated in response to ammonium and oxygen. However, the mechanisms for the regulation of NifA activity are quite different among various nitrogen-fixing bacteria. Unlike the well-studied NifL-NifA regulatory systems in Klebsiella pneumoniae and Azotobacter vinelandii, in Rhodospirillum rubrum NifA is activated by a direct protein-protein interaction with the uridylylated form of GlnB, which in turn causes a conformational change in NifA. We report the identification of several substitutions in the N-terminal GAF domain of R. rubrum NifA that allow NifA to be activated in the absence of GlnB. Presumably these substitutions cause conformational changes in NifA necessary for activation, without interaction with GlnB. We also found that wild-type NifA can be activated in a GlnB-independent manner under certain growth conditions, suggesting that some other effector(s) can also activate NifA. An attempt to use Tn5 mutagenesis to obtain mutants that altered the pool of these presumptive effector(s) failed, though much rarer spontaneous mutations in nifA were detected. This suggests that the necessary alteration of the pool of effector(s) for NifA activation cannot be obtained by knockout mutations. PMID:18757802

  10. TALE-light imaging reveals maternally guided, H3K9me2/3-independent emergence of functional heterochromatin in Drosophila embryos.

    Science.gov (United States)

    Yuan, Kai; O'Farrell, Patrick H

    2016-03-01

    Metazoans start embryogenesis with a relatively naïve genome. The transcriptionally inert, late-replicating heterochromatic regions, including the constitutive heterochromatin on repetitive sequences near centromeres and telomeres, need to be re-established during development. To explore the events initiating heterochromatin formation and examine their temporal control, sequence specificity, and immediate regulatory consequence, we established a live imaging approach that enabled visualization of steps in heterochromatin emergence on specific satellite sequences during the mid-blastula transition (MBT) in Drosophila. Unexpectedly, only a subset of satellite sequences, including the 359-base-pair (bp) repeat sequence, recruited HP1a at the MBT. The recruitment of HP1a to the 359-bp repeat was dependent on HP1a's chromoshadow domain but not its chromodomain and was guided by maternally provided signals. HP1a recruitment to the 359-bp repeat was required for its programmed shift to later replication, and ectopic recruitment of HP1a was sufficient to delay replication timing of a different repeat. Our results reveal that emergence of constitutive heterochromatin follows a stereotyped developmental program in which different repetitive sequences use distinct interactions and independent pathways to arrive at a heterochromatic state. This differential emergence of heterochromatin on various repetitive sequences changes their replication order and remodels the DNA replication schedule during embryonic development. PMID:26915820

  11. Analysis on Optimum Anchorage Angle of Anchoring Bars (Ropes)%锚杆(索)最佳锚固角的取值分析

    Institute of Scientific and Technical Information of China (English)

    蒋明杰

    2013-01-01

      In the protection design of highway slope engineering , the anchorage design of anchoring bars (ropes) are more commonly protection method, while the anchorage angle in the design of anchoring bars (ropes) is a key parameter, which related to the stability and economy in whole anchoring slope engi-neering.Through the influences of anchorage angle on anchored slope engineering safety and economy , the comprehensive analysis is carried out,and the expression formula of optimum anchorage angle is deter-mined, finally through the engineering examples ,it verifies the practicality of optimum anchorage angle .%  在公路边坡工程的防护设计中,锚杆(索)的锚固设计是较常用的防护形式,而在锚杆(索)的设计中锚固角是很关键的一个参数,关系到整个锚固边坡工程的稳定性和经济性。该文通过综合分析锚杆(索)的锚固角对锚固边坡工程的安全性和经济性两方面的影响,确定了锚杆(索)最佳锚固角的表达式,并通过工程实例验证了最佳锚固角的实用性。

  12. Nitrate source indicators in ground water of the Scimitar Subdivision, Peters Creek area, Anchorage, Alaska

    Science.gov (United States)

    Wang, Bronwen; Strelakos, Pat M.; Jokela, Brett

    2000-01-01

    A combination of aqueous chemistry, isotopic measurement, and in situ tracers were used to study the possible nitrate sources, the factors contributing to the spatial distribution of nitrate, and possible septic system influence in the ground water in the Scimitar Subdivision, Municipality of Anchorage, Alaska. Two water types were distinguished on the basis of the major ion chemistry: (1) a calcium sodium carbonate water, which was associated with isotopically heavier boron and with chlorofluorocarbons (CFC's) that were in the range expected from equilibration with the atmosphere (group A water) and (2) a calcium magnesium carbonate water, which was associated with elevated nitrate, chloride, and magnesium concentrations, generally isotopically lighter boron, and CFC's concentrations that were generally in excess of that expected from equilibration with the atmosphere (group B water). Water from wells in group B had nitrate concentrations that were greater than 3 milligrams per liter, whereas those in group A had nitrate concentrations of 0.2 milligram per liter or less. Nitrate does not appear to be undergoing extensive transformation in the ground-water system and behaves as a conservative ion. The major ion chemistry trends and the presence of CFC's in excess of an atmospheric source for group B wells are consistent with waste-water influences. The spatial distribution of the nitrate among wells is likely due to the magnitude of this influence on any given well. Using an expanded data set composed of 16 wells sampled only for nitrate concentration, a significant difference in the static water level relative to bedrock was found. Well water samples with less than 1 milligram per liter nitrate had static water levels within the bedrock, whereas those samples with greater than 1 milligram per liter nitrate had static water levels near or above the top of the bedrock. This observation would be consistent with a conceptual model of a low-nitrate fractured bedrock

  13. Evaluation of the variable anchorage straightwire technique using Ricketts' growth prediction.

    Science.gov (United States)

    Parikakis, Konstantinos A; Moberg, Svante; Hellsing, Eva

    2009-02-01

    The purpose of this retrospective study was to evaluate the treatment effects of the variable anchorage straightwire technique (VAST) in Angle Class II patients using Ricketts' growth prediction analysis. The subjects belonged to two groups: a control, consisting of 30 untreated Class II Swedish individuals (20 girls, 10 boys) with a mean age of 11.2 years, and the other 29 Swedish patients (14 girls, 15 boys), mean age 12.6 years, post-normal and with an increased overbite (OB), treated with the VAST. Two lateral cephalograms were available for every individual. Growth prediction according to Ricketts' visual treatment objective (VTO) was used to estimate the expected growth increments for a 2-year period. It was first used in the control group to determine its validity and then applied to the treated group to evaluate the net effects of treatment. Cephalometric evaluation based on Ricketts' analysis and additional dentoalveolar variables were carried out. Statistical analysis was undertaken using a paired Student's t- and Wilcoxon signed ranks tests. The method of predicting growth according to the VTO was, in general, valid in the untreated subjects, apart from the inclination of the lower incisors, where the proclination had been underestimated. In the treated group, the net effects of treatment were significant for the dentoalveolar variables: reduction of overjet (OJ) and OB, proclination and relative intrusion of the lower incisors, extrusion of the molars, and increase in lower face height. The growth prediction method according to VTO was found to be valid in a sample of Swedish post-normal children concerning skeletal and dentoalveolar variables. The VAST treatment net effects in these growing patients were achieved mainly by dentoalveolar changes. PMID:18832190

  14. CALCULATION ASSESSMENT OF ANCHORAGE IN CONCRETE FOR FIBERGLASS REINFORCEMENT MANUFACTURED IN THE REPUBLIC OF BELARUS

    Directory of Open Access Journals (Sweden)

    E. Ya. Sadin

    2016-01-01

    Full Text Available The paper analyzes advantages and disadvantages of composite reinforcement while reinforcing concrete structures. Results of experimental investigations on adhesion strength of fiberglass reinforcement manufactured in the Republic of Belarus with concrete have been given in the paper. The paper contains an analysis pertaining to the influence of concrete contact diameter and length of fiberglass reinforcing bars on strength and deformability of adhesion with concrete. Failure behavior of prototypes and maximum loading that does cause breakage in adhesion of fiberglass reinforcement with the concrete have been controlled in order to study the influence of diameter and length of fiberglass reinforcing bar contact with the concrete on strength and deformability of concrete adhesion for composite reinforcement produced by various manufacturers. Displacement of non-loaded (free and loaded ends of the reinforcing bars with regard to concrete exposed face on both specimen ends has been also controlled. The experiments have shown that destruction of the prototypes occurred due to initiation of one of the following three reasons: reinforcement sliding in reference to concrete, cleaving of a protective concrete layer or breakage of composite reinforcement. Start-up moments for reinforcing bar slip in relation to the concrete have been determined with the help of two methods. The paper provides proposals for calculation assessment of anchorage in the concrete for fiberglass reinforcement manufactured by the following enterprises and companies “Stroykompozit” (Gomel, Republic of Belarus, Research and Production Company (RPC “Biznes-Kontinent” (Brest, Republic of Belarus and Private Eenterprise (PE “Minplast” (Minsk, Republic of Belarus.

  15. Recorded earthquake responses from the integrated seismic monitoring network of the Atwood Building, Anchorage, Alaska

    Science.gov (United States)

    Celebi, M.

    2006-01-01

    An integrated seismic monitoring system with a total of 53 channels of accelerometers is now operating in and at the nearby free-field site of the 20-story steel-framed Atwood Building in highly seismic Anchorage, Alaska. The building has a single-story basement and a reinforced concrete foundation without piles. The monitoring system comprises a 32-channel structural array and a 21-channel site array. Accelerometers are deployed on 10 levels of the building to assess translational, torsional, and rocking motions, interstory drift (displacement) between selected pairs of adjacent floors, and average drift between floors. The site array, located approximately a city block from the building, comprises seven triaxial accelerometers, one at the surface and six in boreholes ranging in depths from 15 to 200 feet (???5-60 meters). The arrays have already recorded low-amplitude shaking responses of the building and the site caused by numerous earthquakes at distances ranging from tens to a couple of hundred kilometers. Data from an earthquake that occurred 186 km away traces the propagation of waves from the deepest borehole to the roof of the building in approximately 0.5 seconds. Fundamental structural frequencies [0.58 Hz (NS) and 0.47 Hz (EW)], low damping percentages (2-4%), mode coupling, and beating effects are identified. The fundamental site frequency at approximately 1.5 Hz is close to the second modal frequencies (1.83 Hz NS and 1.43 EW) of the building, which may cause resonance of the building. Additional earthquakes prove repeatability of these characteristics; however, stronger shaking may alter these conclusions. ?? 2006, Earthquake Engineering Research Institute.

  16. Impulse control and underlying functions of the left DLPFC mediate age-related and age-independent individual differences in strategic social behavior.

    Science.gov (United States)

    Steinbeis, Nikolaus; Bernhardt, Boris C; Singer, Tania

    2012-03-01

    Human social exchange is often characterized by conflicts of interest requiring strategic behavior for their resolution. To investigate the development of the cognitive and neural mechanisms underlying strategic behavior, we studied children's decisions while they played two types of economic exchange games with differing demands of strategic behavior. We show an increase of strategic behavior with age, which could not be explained by age-related changes in social preferences but instead by developmental differences in impulsivity and associated brain functions of the left dorsolateral prefrontal cortex (DLPFC). Furthermore, observed differences in cortical thickness of lDLPFC were predictive of differences in impulsivity and strategic behavior irrespective of age. We conclude that egoistic behavior in younger children is not caused by a lack of understanding right or wrong, but by the inability to implement behavioral control when tempted to act selfishly; a function relying on brain regions maturing only late in ontogeny.

  17. ACE inhibition with spirapril improves diastolic function at rest independent of vasodilation during treatment with spirapril in mild to moderate hypertension

    DEFF Research Database (Denmark)

    Petersen, J R; Drabaek, H; Fornitz, Gitte Gleerup;

    1996-01-01

    .44-1.25), and the drug normalized the A/E-ratio VTI in those patients with elevated values. The hemodynamic variables, left ventricular mass, and end-systolic wall stress were unchanged during all three treatments. There were no significant changes in mean blood pressure during the treatment periods. These results...... indicate that spirapril lowers A/E ratio within four weeks in patients with mild to moderate essential hypertension. It thereby seems able to improve left ventricular diastolic function. The effect is not dependent upon changes in hemodynamic variables, blood pressure, left ventricular mass, or end......The effects of the ACE inhibitor spirapril and of hydrochlorothiazide on left ventricular diastolic function were studied. Thirteen patients with mild to moderate essential hypertension completed this randomized, double-blinded, placebo-controlled, crossover study. After a three-week run-in period...

  18. Identification of an MRAP-Independent Melanocortin-2 Receptor: Functional Expression of the Cartilaginous Fish, Callorhinchus milii, Melanocortin-2 Receptor in CHO Cells

    OpenAIRE

    Reinick, Christina L.; Liang, Liang; Angleson, Joseph K.; Dores, Robert M.

    2012-01-01

    Phylogenetic analyses indicate that the genome of the cartilaginous fish, Callorhynchus milii (elephant shark), encodes a melanocortin-2 receptor (MC2R) ortholog. Expression of the elephant shark mc2r cDNA in Chinese hamster ovary (CHO) cells revealed that trafficking to the plasma membrane and functional activation of the receptor do not require coexpression with an exogenous melanocortin receptor-2 accessory protein (mrap) cDNA. Ligand selectivity studies indicated that elephant shark MC2R-...

  19. Muscular weakness represents the main limiting factor of walk, functional independence and quality of life of myelopathy patients associated to HTLV-1

    Directory of Open Access Journals (Sweden)

    Renata Costa Caiafa

    2016-04-01

    Full Text Available ABSTRACT HTLV-1-associated myelopathy is a progressive disabling disease associated with gait abnormalities. Objective To identify and quantify the main muscles affected by weakness and spasticity, their impact on gait, functional capacity and on quality of life of HTLV-1-associated myelopathy patients. Method We evaluated lower limbs muscular strength according to the Medical Research Council scale, spasticity according to the modified Ashworth scale, daily activities according to the Barthel Index and quality of life according to the Short-Form Health Survey-36 of 26 HTLV-1-associated myelopathy patients. Results The muscles most affected by weakness included the dorsal flexors and knee flexors. Spasticity predominated in the hip adductor muscles and in plantar flexors. Assistance for locomotion, minimal dependence in daily activities, limitations in functional capacity and physical aspects were the most common findings. Conclusion The impairment of gait, functional dependence and quality of life were predominantly a consequence of intense muscle weakness in HTLV-1-associated myelopathy patients.

  20. Cell-free synthesis of functional human epidermal growth factor receptor: Investigation of ligand-independent dimerization in Sf21 microsomal membranes using non-canonical amino acids

    Science.gov (United States)

    Quast, Robert B.; Ballion, Biljana; Stech, Marlitt; Sonnabend, Andrei; Varga, Balázs R.; Wüstenhagen, Doreen A.; Kele, Péter; Schiller, Stefan M.; Kubick, Stefan

    2016-01-01

    Cell-free protein synthesis systems represent versatile tools for the synthesis and modification of human membrane proteins. In particular, eukaryotic cell-free systems provide a promising platform for their structural and functional characterization. Here, we present the cell-free synthesis of functional human epidermal growth factor receptor and its vIII deletion mutant in a microsome-containing system derived from cultured Sf21 cells. We provide evidence for embedment of cell-free synthesized receptors into microsomal membranes and asparagine-linked glycosylation. Using the cricket paralysis virus internal ribosome entry site and a repetitive synthesis approach enrichment of receptors inside the microsomal fractions was facilitated thereby providing analytical amounts of functional protein. Receptor tyrosine kinase activation was demonstrated by monitoring receptor phosphorylation. Furthermore, an orthogonal cell-free translation system that provides the site-directed incorporation of p-azido-L-phenylalanine is characterized and applied to investigate receptor dimerization in the absence of a ligand by photo-affinity cross-linking. Finally, incorporated azides are used to generate stable covalently linked receptor dimers by strain-promoted cycloaddition using a novel linker system. PMID:27670253

  1. The two tryptophans of β2-microglobulin have distinct roles in function and folding and might represent two independent responses to evolutionary pressure

    Directory of Open Access Journals (Sweden)

    Monti Maria

    2011-06-01

    Full Text Available Abstract Background We have recently discovered that the two tryptophans of human β2-microglobulin have distinctive roles within the structure and function of the protein. Deeply buried in the core, Trp95 is essential for folding stability, whereas Trp60, which is solvent-exposed, plays a crucial role in promoting the binding of β2-microglobulin to the heavy chain of the class I major histocompatibility complex (MHCI. We have previously shown that the thermodynamic disadvantage of having Trp60 exposed on the surface is counter-balanced by the perfect fit between it and a cavity within the MHCI heavy chain that contributes significantly to the functional stabilization of the MHCI. Therefore, based on the peculiar differences of the two tryptophans, we have analysed the evolution of β2-microglobulin with respect to these residues. Results Having defined the β2-microglobulin protein family, we performed multiple sequence alignments and analysed the residue conservation in homologous proteins to generate a phylogenetic tree. Our results indicate that Trp60 is highly conserved, whereas some species have a Leu in position 95; the replacement of Trp95 with Leu destabilizes β2-microglobulin by 1 kcal/mol and accelerates the kinetics of unfolding. Both thermodynamic and kinetic data fit with the crystallographic structure of the Trp95Leu variant, which shows how the hydrophobic cavity of the wild-type protein is completely occupied by Trp95, but is only half filled by Leu95. Conclusions We have established that the functional Trp60 has been present within the sequence of β2-microglobulin since the evolutionary appearance of proteins responsible for acquired immunity, whereas the structural Trp95 was selected and stabilized, most likely, for its capacity to fully occupy an internal cavity of the protein thereby creating a better stabilization of its folded state.

  2. pRB-Dependent, J Domain-Independent Function of Simian Virus 40 Large T Antigen in Override of p53 Growth Suppression

    OpenAIRE

    Gjoerup, Ole; Chao, Herta; DeCaprio, James A.; Roberts, Thomas M.

    2000-01-01

    Simian virus 40 (SV40) large T antigen (LT) can immortalize and transform many cell types. These activities are attributed in large part to the binding and functional inactivation by LT of two major tumor suppressors: p53 and the retinoblastoma protein, pRB. Most effects of LT on pRB have been shown to additionally require an intact J domain, which mediates binding to Hsc70. We show here that the J domain is not required for p53 override in full-length LT. Although LT binds p53, it was shown ...

  3. Combining oxygen plasma treatment with anchorage of cationized gelatin for enhancing cell affinity of poly(lactide-co-glycolide).

    Science.gov (United States)

    Shen, Hong; Hu, Xixue; Yang, Fei; Bei, Jianzhong; Wang, Shenguo

    2007-10-01

    Surface characteristics greatly influence attachment and growth of cells on biomaterials. Although polylactone-type biodegradable polymers have been widely used as scaffold materials for tissue engineering, lack of cell recognition sites, poor hydrophilicity and low surface energy lead to a bad cell affinity of the polymers, which limit the usage of polymers as scaffolds in tissue engineering. In the present study, surface of poly (L-lactide-co-glycolide) (PLGA) was modified by a method of combining oxygen plasma treatment with anchorage of cationized gelatin. Modification effect of the method was compared with other methods of oxygen plasma treatment, cationized gelatin or gelatin coating and combining oxygen plasma treatment with anchorage of gelatin. The change of surface property was compared by contact angles, surface energy, X-ray photoelectron spectra (XPS), attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR) and scanning electron microscopy (SEM) measurement. The optimum oxygen pretreatment time determined by surface energy was 10 min when the power was 50 W and the oxygen pressure was 20 Pa. Analysis of the stability of gelatin and cationized gelatin anchored on PLGA by XPS, ATR-FTIR, contact angles and surface energy measurement indicated the cationized gelatin was more stable than gelatin. The result using mouse NIH 3T3 fibroblasts as model cells to evaluate cell affinity in vitro showed the cationized gelatin-anchored PLGA (OCG-PLGA) was more favorable for cell attachment and growth than oxygen plasma treated PLGA (O-PLGA) and gelatin-anchored PLGA (OG-PLGA). Moreover cell affinity of OCG-PLGA could match that of collagen-anchored PLGA (AC-PLGA). So the surface modification method combining oxygen plasma treatment with anchorage of cationized gelatin provides a universally effective way to enhance cell affinity of polylactone-type biodegradable polymers. PMID:17618682

  4. Intrusão dentária utilizando mini-implantes Orthodontic intrusion with mini-implant anchorage

    Directory of Open Access Journals (Sweden)

    Telma Martins de Araújo

    2008-10-01

    Full Text Available INTRODUÇÃO: dentre os diversos tipos de movimentos dentários induzidos ortodonticamente, o de intrusão é, sem dúvida, um dos mais difíceis de serem conseguidos. A mecânica intrusiva convencional, apesar de viável, é complexa, no que diz respeito ao controle de seus efeitos colaterais. Isso, em grande parte, refere-se à dificuldade em se obter uma ancoragem satisfatória. Neste contexto, os mini-implantes, por oferecerem efetiva ancoragem esquelética, têm se mostrado de extrema valia para os ortodontistas, tornando a intrusão, tanto de dentes anteriores quanto posteriores, um procedimento cada vez mais simples, do ponto de vista mecânico. OBJETIVO: pretende-se, então, neste artigo, descrever e demonstrar, clinicamente, as diversas possibilidades de utilização dos mini-implantes como recurso de ancoragem para o movimento de intrusão.INTRODUCTION: Among all different varieties of orthodontically induced tooth movement, intrusion is, without doubt, one of the most difficult movements to be reached. Conventional intrusive biomechanics, although possible, may lead to undesirable side-effects. These events, in most cases, are related to the difficulty in obtaining an efficient anchorage unit. Then, mini-implants, because of their high success rates of effective skeletal anchorage, are valuable devices to orthodontists, transforming the intrusion movement, both at the anterior and the posterior regions, a more and more uncomplicated biomechanical procedure. AIM: to describe and show, clinically, different ways of using mini-implants as an anchorage system to intrusion movement.

  5. Gestational Diabetes Mellitus Worsens the Profile of Cardiometabolic Risk Markers and Decrease Indexes of Beta-Cell Function Independently of Insulin Resistance in Nondiabetic Women with a Parental History of Type 2 Diabetes

    Directory of Open Access Journals (Sweden)

    Alina Sokup

    2014-01-01

    Full Text Available Background. Women with a history of both parental type 2 diabetes (pt2DM and previous gestational diabetes (pGDM represent a group at high risk of cardiovascular events. We hypothesized that pGDM changes cardiometabolic risk markers levels as well as theirs associations with glucose indices in nondiabetic pt2DM women. Methods. Anthropometric parameters, glucose regulation (OGTT, insulin resistance (HOMA-IR, beta-cell function, lipid levels, parameters of endothelial dysfunction, and inflammation were evaluated in 55 women with pt2DM, 40 with both pt2DM and pGDM 2–24 months postpartum, and 35 controls. Results. Prediabetes was diagnosed more frequently in women with both pt2DM and pGDM in comparison with women with only pt2DM (10 versus 8, P=0.04. The pGDM group had higher LDL-cholesterol, sICAM-1, tPa Ag, fibrinogen, and lower beta-cell function after adjustment for HOMA-IR, in comparison with pt2DM group. In pt2DM group postchallenge glucose correlated independently with hsCRP and in pGDM group fasting glucose with HOMA-IR. Conclusions. pGDM exerts a combined effect on cardiometabolic risk markers in women with pt2DM. In these women higher LDL-cholesterol, fibrinogen, sICAM-1, tPa Ag levels and decreased beta cell function are associated with pGDM independently of HOMA-IR index value. Fasting glucose is an important cardiometabolic risk marker and is independently associated with HOMA-IR.

  6. Independent and Combined Association of Muscle Strength and Cardiorespiratory Fitness in Youth With Insulin Resistance and β-Cell Function in Young Adulthood

    DEFF Research Database (Denmark)

    Grøntved, Anders; Ried-Larsen, Mathias; Ekelund, Ulf;

    2013-01-01

    ergometer test. Insulin resistance (homeostasis model assessment of insulin resistance [HOMA-IR]) and β-cell function (homeostasis model assessment of β-cell function [HOMA-B]) were estimated from fasting serum insulin and glucose that were obtained in youth and at follow-up in young adulthood.......RESULTSFor each 1-SD difference in isometric muscle strength (0.16 N/kg) in youth, fasting insulin, HOMA-IR, and HOMA-B in young adulthood changed with -11.3% (95% CI, -17.0 to -5.2), -12.2% (-18.2 to -5.7), and -8.9% (-14.4 to -3.0), respectively, in young adulthood after adjustment for CRF and personal...... lifestyle and demographic factors. Results for CRF were very similar in magnitude, and the magnitude of associations for both exposures was unchanged with additional adjustment for general or abdominal adiposity in youth. Combined associations of muscle strength and CRF with fasting insulin, HOMA...

  7. On the k-independence required by linear probing and minwise independence

    DEFF Research Database (Denmark)

    Pǎtraşcu, Mihai; Thorup, Mikkel

    2016-01-01

    We show that linear probing requires 5-independent hash functions for expected constant-time performance, matching an upper bound of Pagh et al. [2009]. More precisely, we construct a random 4-independent hash function yielding expected logarithmic search time for certain keys. For (1 + ε......)-approximate minwise independence, we show that Ω(lg1 ε)-independent hash functions are required, matching an upper bound of Indyk [2001]. We also show that the very fast 2-independent multiply-shift scheme of Dietzfelbinger [1996] fails badly in both applications....

  8. Dependence and Independence

    OpenAIRE

    Grädel, E.; Väänänen, J.

    2010-01-01

    We introduce an atomic formula intuitively saying that given variables are independent from given other variables if a third set of variables is kept constant. We contrast this with dependence logic. We show that our independence atom gives rise to a natural logic capable of formalizing basic intuitions about independence and dependence.

  9. 微种植体初期稳定性的影响因素%Factors affecting primary stability of mini-implant anchorage

    Institute of Scientific and Technical Information of China (English)

    鲁颖娟; 常少海

    2013-01-01

    Mini implant anchorage for the orthodontic treatment is an effective method, the primary stability is critical in the achievement of reliable anchorage. Therefore, it is the purpose of this review to summarize the cur-rent literature on the primary stability of mini-implant anchorage, in order to improve overall implant success.%  微种植体支抗技术是正畸治疗中一种有效和理想的方法,其初期稳定性是获得可靠支抗的必要条件。本文就影响微种植体支抗初期稳定性的患者因素、微种植体因素和操作因素等研究进展作一综述,以提高微种植体支抗的成功率。

  10. 微型种植体骨性支抗在临床的应用研究%The application and research of micro-implant bone anchorage in clinic

    Institute of Scientific and Technical Information of China (English)

    苏丽英; 刘国实; 王松梅; 张宏; 卢晓梅; 陈竟男; 夏国栋; 李光磊

    2011-01-01

    -drilling miniscrew implants as skeletal anchorage with a small contact area, stability, strength and strong advantage; planted immediate loading, shorter course of treatment; orthodontic doctors can operate independently, patients are also easy to accept.

  11. Central Bank independence

    Directory of Open Access Journals (Sweden)

    Vasile DEDU

    2012-08-01

    Full Text Available In this paper we present the key aspects regarding central bank’s independence. Most economists consider that the factor which positively influences the efficiency of monetary policy measures is the high independence of the central bank. We determined that the National Bank of Romania (NBR has a high degree of independence. NBR has both goal and instrument independence. We also consider that the hike of NBR’s independence played an important role in the significant disinflation process, as headline inflation dropped inside the targeted band of 3% ± 1 percentage point recently.

  12. 种植体支抗在正畸临床上的应用%Implant Anchorage in Orthodontic Clinical Application

    Institute of Scientific and Technical Information of China (English)

    刘丽梅

    2015-01-01

    目的:探讨种植体支抗在正畸临床治疗。方法对20例种植体支抗的正畸治疗应用方法及临床应用体会。结果20例种植体支抗,微镙钉种植16例,钛板种植体3例,修复种植体1例。种植体均保持了稳定,种植体周围软组织健康,脱落率10%。结论牙齿的缺失造成支抗的不足,某些类型的牙齿移动难以或不能完成。将钛合金牙种植体作为支抗完成正畸治疗,正畸治疗结束后,将种植体行永久性修复。%Objective To investigate the implant anchorage in orthodontic clinical treatment. Methods 20 cases of implant anchorage orthodontic treatment application methods and clinical application experience. Results 20 cases of implant anchorage, micro screws planting 16 cases, 3 cases of titanium implants , implant restoration one case . Implants have maintained a stable implant soft tissue health, loss rate of 10%. Conclusion The lack of teeth caused by lack of anchorage, and certain types of tooth movement difficult or impossible to complete. The titanium dental implants as orthodontic anchorage is completed, after the end of orthodontic treatment, the implant permanently repair the line, traditional dental implant anchorage to be more widely used in orthodontic patients with a combination therapy of implant restoration.

  13. The visual representations of motion and of gravity are functionally independent: Evidence of a differential effect of smooth pursuit eye movements.

    Science.gov (United States)

    De Sá Teixeira, Nuno Alexandre

    2016-09-01

    The memory for the final position of a moving object which suddenly disappears has been found to be displaced forward, in the direction of motion, and downwards, in the direction of gravity. These phenomena were coined, respectively, Representational Momentum and Representational Gravity. Although both these and similar effects have been systematically linked with the functioning of internal representations of physical variables (e.g. momentum and gravity), serious doubts have been raised for a cognitively based interpretation, favouring instead a major role of oculomotor and perceptual factors which, more often than not, were left uncontrolled and even ignored. The present work aims to determine the degree to which Representational Momentum and Representational Gravity are epiphenomenal to smooth pursuit eye movements. Observers were required to indicate the offset locations of targets moving along systematically varied directions after a variable imposed retention interval. Each participant completed the task twice, varying the eye movements' instructions: gaze was either constrained or left free to track the targets. A Fourier decomposition analysis of the localization responses was used to disentangle both phenomena. The results show unambiguously that constraining eye movements significantly eliminates the harmonic components which index Representational Momentum, but have no effect on Representational Gravity or its time course. The found outcomes offer promising prospects for the study of the visual representation of gravity and its neurological substrates. PMID:27106480

  14. Characterization of an Arabidopsis-Phytophthora pathosystem: resistance requires a functional PAD2 gene and is independent of salicylic acid, ethylene and jasmonic acid signalling.

    Science.gov (United States)

    Roetschi, A; Si-Ammour, A; Belbahri, L; Mauch, F; Mauch-Mani, B

    2001-11-01

    Arabidopsis accessions were screened with isolates of Phytophthora porri originally isolated from other crucifer species. The described Arabidopsis-Phytophthora pathosystem shows the characteristics of a facultative biotrophic interaction similar to that seen in agronomically important diseases caused by Phytophthora species. In susceptible accessions, extensive colonization of the host tissue occurred and sexual and asexual spores were formed. In incompatible combinations, the plants reacted with a hypersensitive response (HR) and the formation of papillae at the sites of attempted penetration. Defence pathway mutants such as jar1 (jasmonic acid-insensitive), etr1 (ethylene receptor mutant) and ein2 (ethylene-insensitive) remained resistant towards P. porri. However, pad2, a mutant with reduced production of the phytoalexin camalexin, was hyper-susceptible. The accumulation of salicylic acid (SA) and PR1 protein was strongly reduced in pad2. Surprisingly, this lack of SA accumulation does not appear to be the cause of the hyper-susceptibility because interference with SA signalling in nahG plants or sid2 or npr1 mutants had only a minor effect on resistance. In addition, the functional SA analogue benzothiadiazol (BTH) did not induce resistance in susceptible plants including pad2. Similarly, the complete blockage of camalexin biosynthesis in pad3 did not cause susceptibility. Resistance of Arabidopsis against P. porri appears to depend on unknown defence mechanisms that are under the control of PAD2.

  15. Mini-implant Anchorage in Orthodontic Treatment Nursing Intervention%微型种植支抗在口腔正畸治疗中的护理干预

    Institute of Scientific and Technical Information of China (English)

    龚晓琳

    2014-01-01

    Objective To summarize the micro-implant anchorage methods and ef ects of nursing interventions in orthodontic treatment. Methods Our hospital in January 2012-2013 February malocclusion treated 45 patients were given orthodontic mini-implant anchorage surgery before and after surgery have implemented measures to improve nursing intervention period was 14 days. Patient satisfaction through 9 Surgical cosmetic surgery orthodontic clinical data analysis. Results After the intervention ef ective in 34 cases, ef ective in 11 cases, the ef ective rate was 100.0%, with no serious complications and at achment loss occurs. After 14 days in patients with plaque index increased significantly, while the probing depth decreased with preoperative contrast significantly dif erent ( <0.05). Conclusion The mini implant anchorage in orthodontic treatment applied to ef ectively improve the appearance of the mouth and chewing function, but also the need to strengthen nursing intervention.%目的:总结微型种植支抗在口腔正畸治疗中的护理干预方法与效果。方法选择我院正畸科2012年1月~2013年2月收治的错颌畸形患者45例,都给予口腔正畸微型种植支抗手术治疗,在手术前后都实行了完善的护理措施,干预周期为14d。通过对患者进行临床资料分析。结果干预后显效34例,有效11例,有效率为100.0%,无严重并发症与附着丧失发生。术后14d患者的菌斑指数明显下降,而探诊深度明显下降,与术前对比差异明显(<0.05)。结论微型种植支抗在口腔正畸治疗中的应用有效改善了口腔美观及咀嚼功能,但是也需要加强护理干预。

  16. On Clinical Stability Factors Affecting Micro Implant Anchorage%影响微种植钉支抗的临床稳定性因素研究

    Institute of Scientific and Technical Information of China (English)

    包晨刚

    2014-01-01

    Objective To investigate the stability of micro implant as reinforcing anchorage method in the clinical application,at the same time to analyze the related factors of the success,to provide the reference for the clinical work in the future. Methods 53 patients were implanted 171 micro screws, observation period of 1 years,the influence of age,gender,the mandibular plane angle,periodontitis,periodontal tissue swelling history on stability of micro implant were analyzed. Results The total success rate of 53 patients with wrong micro implant was 90. 57%(48/53),the oral hygiene status,patient’ s periodontal tissue swelling,periodontitis,mandibular plane angle history are independent factors of micro implant survival rate,the effect of the age,gender and micro implant design factors on micro screw success rate had no significant difference,it was not statistically significant (P > 0. 05). Conclusion Patients with micro implant as reinforcing anchorage means should pay attention to keeping the mouth clean, physicians should design planting scheme according to the mandibular plane angle, avoid the periodontal tissue swelling occurs in order to improve the success rate of micro implant.%目的:探讨微型种植钉作为增强支抗手段在临床应用中的稳定性,同时分析影响其成功率的相关因素,为今后的临床工作提供参考.方法53例错颌患者共植入微型种植钉171枚,观察期1 a,并分析年龄、性别、下颌平面角、牙周组织肿胀、牙周炎史等因素对微型种植钉稳定性的影响.结果本组53例错颌患者的微型种植钉总成功率为90.57%(48/53),其中患者的口腔卫生状况、牙周组织肿胀、牙周炎史、下颌平面角等是微型种植钉成活率的独立影响因素,而且患者的年龄、性别以及微型种植钉的设计因素对微型种植钉成功率的影响无显著性差异,不具有统计学意义(P>0.05).结论采用微型种植钉作为增强支抗手段的患者要注意

  17. Efeitos da natação sobre a independência funcional de pacientes com lesão medular Efectos de la natación sobre la independencia funcional de pacientes con lesión medular Effects of swimming on the functional independence of patients with spinal cord injury

    Directory of Open Access Journals (Sweden)

    Maurício Corte Real da Silva

    2005-08-01

    puntaje total. CONCLUSIÓN: La actividad de natación fue efectiva en la mejora de la condición física, trayendo beneficios motores sobre las habilidades funcionales de los participantes del grupo experimental.FUNDAMENTS AND OBJECTIVE: Sports play an important role in the physical rehabilitation of patients with spinal cord injury by broadening the therapeutic alternatives in addition to providing functional independence. The objective of this study is to determining the effects of swimming on the functional capacity of patients with spinal cord injury. METHODS: 16 patients with spinal cord injury were divided into two groups: experimental and control. The assessment of both groups was performed using the Functional Independence Measure (FIM scale before and after the trial, which consisted of swimming sessions performed twice a week during a period of four months. RESULTS: Both groups demonstrated noticeable gains related to body care, transference, overall and motor scores, even though the experimental group presented greater gains in transference, overall motor score and overall score. CONCLUSION: The swimming activity was effective in improving their physical condition, bringing motor benefits on the functional capacity of participants from the experimental group.

  18. Board independence and innovation

    OpenAIRE

    Coelho, Mariana Campos Carvalho

    2015-01-01

    Legislation introduced in the U.S. in 2002/2003 significantly changed board composition of public firms by imposing a 50% independent directors’ ratio. Research on the effect of independent directors is not consensual, implying that this exogenous shock is a unique opportunity to study their importance. This study answers the question of whether or not independent directors can effectively mitigate agency conflicts between shareholders and the management, having a positive impact on the choic...

  19. What is Stochastic Independence?

    OpenAIRE

    Franz, Uwe

    2002-01-01

    The notion of a tensor product with projections or with inclusions is defined. It is shown that the definition of stochastic independence relies on such a structure and that independence can be defined in an arbitrary category with a tensor product with inclusions or projections. In this context, the classifications of quantum stochastic independence by Muraki, Ben Ghorbal, and Sch\\"urmann become classifications of the tensor products with inclusions for the categories of algebraic probabilit...

  20. Functional independence in patients with chronic kidney disease being treated with haemodialysis¹ Independencia funcional en pacientes con enfermedad renal crónica en tratamiento de hemodiálisis Independência funcional em pacientes com doença renal crônica em tratamento hemodialítico

    Directory of Open Access Journals (Sweden)

    Graziella Allana Serra Alves de Oliveira Oller

    2012-12-01

    Full Text Available PURPOSE: This study has described and analysed the functional independence of the patients served in the haemodialysis services of a countryside town in the State of São Paulo, Brazil, using the Functional Independence Measure (FIM. METHOD: The population considered was that of 214 patients being treated with haemodialysis, assessed in 2011, by means of a social, demographic and clinical report, a Mini-Mental State Examination (MMSE and also the FIM. RESULTS: The mean age of the population under study was 58.01 years, while the mean FIM point score was 118.38 points, showing a level of complete or modified independence within this population. Even though the level of dependence found has been low, this can be highlighted, within the locomotion domain, in the activity of going up and down stairs (10.28%. Age, complications arising from haemodialysis, and comorbidities show a negative correlation with FIM. CONCLUSION: Awareness of the level of functional independence of the patients being subjected to treatment with haemodialysis is essential in order to back up intervention for the improvement of nursing assistance provided to this population.OBJETIVO: este estudio describió y analizó la independencia funcional de los pacientes atendidos en los servicios de hemodiálisis de una ciudad del interior paulista, según la Medida de Independencia Funcional (MIF. MÉTODO: la población fue de 214 pacientes en tratamiento de hemodiálisis, evaluados en 2011, por medio de un cuestionario sociodemográfico y clínico, Mini examen del Estado Mental y MIF. RESULTADOS: la edad Media de la población del estudio fue de 58,01 años y la media de la MIF fue de 118,38 puntos, evidenciando un nivel de independencia completa o modificada de esa población. Mismo habiendo sido bajo el nivel de dependencia encontrado, se destacó en el dominio locomoción, en la actividad ascender y bajar escaleras (10,28%. La edad, complicaciones relacionadas a la hemodiálisis y

  1. Gravity Independent Compressor Project

    Data.gov (United States)

    National Aeronautics and Space Administration — We propose to develop and demonstrate a small, gravity independent, vapor compression refrigeration system using a linear motor compressor which effectively...

  2. Nível de independência funcional e déficit cognitivo em idosos com doença de Alzheimer Nivel de independencia funcional y déficit cognitivo en ancianos con enfermedade de Alzheimer Functional independence level and cognitive deficit in elderly individuals with Alzheimer's disease

    Directory of Open Access Journals (Sweden)

    Luana Flávia da Silva Talmelli

    2010-12-01

    Full Text Available Este estudo investigou a influência do nível de independência funcional dos idosos com doença de Alzheimer segundo escores da avaliação cognitiva. A população foi composta por 67 idosos atendidos no Ambulatório de Neurologia Comportamental do Hospital das Clínicas/Ribeirão Preto, avaliados em 2008, por meio de um questionário para dados sociodemográficos, Medida de Independência Funcional (MIF e Mini-Exame do Estado Mental (MEEM. Observou-se que o déficit cognitivo influenciou o desempenho na realização das AVDs. A média da MIF para idosos sem déficit cognitivo foi 107,7 e para os com déficit, 63,2 (pEste estudio investigó la influencia del nivel de independencia funcional de los ancianos con Enfermedad de Alzheimer según puntajes de evaluación cognitiva. La población se compuso de 67 ancianos atendidos en el Ambulatorio de Neurología Comportamental del Hospital das Clínicas/Ribeirão Preto/SP/Brasil, evaluados en 2008 a través de un cuestionario para datos sociodemográficos, Medida de Independencia Funcional (MIF y Mini Examen de Estado Mental (MEEM. Se observó que el déficit cognitivo influyó en el desempeño en la realización de las AVDs. La media de la MIF para ancianos sin déficit cognitivo fue de 107,7% y para aquellos con déficit, 63,2% (pThis study investigated the influence of the functional independence level of elderly individuals with Alzheimer's disease, according to cognitive assessment scores. Participants were 67 elderly individuals who received care in the Behavioral Neurology Outpatient Clinic of Hospital das Clinicas in Ribeirão Preto. Participants were evaluated in 2008 through a questionnaire for socio-demographic data, Functional Independence Measure (FIM and the mini-mental state examination (MMSE. The cognitive deficit influenced the performance in carrying out activities of daily living. The average FIM for elderly people without cognitive deficit was 107.7 and for individuals with

  3. Auditors and Independence

    OpenAIRE

    World Bank

    2006-01-01

    Independence is a concept of fundamental importance in business relations and corporate governance. It is above all crucial in auditing, which provides a guarantee of the integrity of the numbers on which an efficient allocation of capital within the capital market system depends. The job of auditors is to express an independent opinion on whether financial statements are reliable and cred...

  4. Fostering Musical Independence

    Science.gov (United States)

    Shieh, Eric; Allsup, Randall Everett

    2016-01-01

    Musical independence has always been an essential aim of musical instruction. But this objective can refer to everything from high levels of musical expertise to more student choice in the classroom. While most conceptualizations of musical independence emphasize the demonstration of knowledge and skills within particular music traditions, this…

  5. Brefeldin A Reduces Anchorage-Independent Survival, Cancer Stem Cell Potential and Migration of MDA-MB-231 Human Breast Cancer Cells

    Directory of Open Access Journals (Sweden)

    Chao-Neng Tseng

    2014-10-01

    Full Text Available Cancer stem cells (CSCs are a subset of cancer cells in tumors or established cancer cell lines that can initiate and sustain the growth of tumors in vivo. Cancer stem cells can be enriched in serum-free, suspended cultures that allow the formation of tumorspheres over several days to weeks. Brefeldin A (BFA is a mycotoxin that induces endoplasmic reticulum (ER stress in eukaryotic cells. We found that BFA, at sub-microgram per milliliter concentrations, preferentially induced cell death in MDA-MB-231 suspension cultures (EC50: 0.016 µg/mL compared to adhesion cultures. BFA also effectively inhibited clonogenic activity and the migration and matrix metalloproteinases-9 (MMP-9 activity of MDA-MB-231 cells. Western blotting analysis indicated that the effects of BFA may be mediated by the down-regulation of breast CSC marker CD44 and anti-apoptotic proteins Bcl-2 and Mcl-1, as well as the reversal of epithelial-mesenchymal transition. Furthermore, BFA also displayed selective cytotoxicity toward suspended MDA-MB-468 cells, and suppressed tumorsphere formation in T47D and MDA-MB-453 cells, suggesting that BFA may be effective against breast cancer cells of various phenotypes.

  6. Transformation of primary human embryonic kidney cells to anchorage independence by a combination of BK virus DNA and the Harvey-ras oncogene

    International Nuclear Information System (INIS)

    Primary human embryonic kidney (HEK) cells were transformed by a focus assay with BK virus (BKV) DNA molecularly cloned at its unique EcoRI site. Both viral DNA sequences and viral tumor antigens were present and expressed in all the foci that the authors examined. However, cells isolated from foci were incapable of growth in soft agar. They then examined the transformation of HEK cells after their transfection with a combination of BKV DNA and either the normal or the activated form of the human Ha-ras oncogene (EJ c-Ha-ras-1). Only the cells transfected with a combination of BKV DNA and the activated form of Ha-ras DNAs were present in the transformed colonies. BKV tumor antigens and the Ha-ras p21 protein were also expressed

  7. Remains of the ancient ports and anchorage points at Miyani and Visawada, on the west coast of India: A study based on underwater investigations

    Digital Repository Service at National Institute of Oceanography (India)

    Gaur, A.S.; Sundaresh; Tripati, S.

    as an excellent natural harbour. The important antiquities include varieties of stone anchors, which are very similar to those reported from Dwarka and Bet Dwarka. The identification of ancient anchorage points between water depth of 5 and 15 m is another...

  8. Anchorage of TiO2-blasted, HA-coated, and machined implants: an experimental study with rabbits

    DEFF Research Database (Denmark)

    Gotfredsen, K; Wennerberg, A; Johansson, C;

    1995-01-01

    The purpose of this study was to evaluate the histometrical and biomechanical anchorage of TiO2-blasted implants and TiO2-blasted implants coated with hydroxyapatite. The control implants were machined. Twenty-six rabbits had a total of 156 implants placed in the proximal part of the tibia. Each...... rabbit had a machined, a TiO2-blasted, and a TiO2-blasted, HA-coated implant placed in each tibia. After a healing period of 3 and 12 weeks, respectively, the implants placed in the right tibia were used for removal torque test, and the implants placed in the left tibia were used for histomorphometrical...

  9. [Low-cost simple anchorage systems in the removable hybrid prosthesis. Locator Root Attachment and Würzburg post].

    Science.gov (United States)

    Teubner, Eckart; Galindo, Martha L; Arnold, Dario; Marinello, Carlo P

    2009-01-01

    For a simple and provisional retention of a removable prosthesis, less expensive direct retainers are an option compared to indirect cast gold copings with attachment. The Dalbo-Rotex-retainer and the Ticap-system are clinically established. The Locator Root attachment and the Würzburger Stift were recently introduced. The Locator Root attachment uses a massive profiled cylindrical post as a radicular anchorage. The Würzburger Stift has a small endodontic part which is retained by four spreadable lamellae in a convergent cavity. They further differ in the design of the male and female part. Depending on the clinical situation these characteristics can offer benefits and disadvantages. Both systems are documented and their indication, advantages and restrictions are discussed with clinical relevance. PMID:20112639

  10. [Low-cost simple anchorage systems in the removable hybrid prosthesis. Locator Root Attachment and Würzburg post].

    Science.gov (United States)

    Teubner, Eckart; Galindo, Martha L; Arnold, Dario; Marinello, Carlo P

    2009-01-01

    For a simple and provisional retention of a removable prosthesis, less expensive direct retainers are an option compared to indirect cast gold copings with attachment. The Dalbo-Rotex-retainer and the Ticap-system are clinically established. The Locator Root attachment and the Würzburger Stift were recently introduced. The Locator Root attachment uses a massive profiled cylindrical post as a radicular anchorage. The Würzburger Stift has a small endodontic part which is retained by four spreadable lamellae in a convergent cavity. They further differ in the design of the male and female part. Depending on the clinical situation these characteristics can offer benefits and disadvantages. Both systems are documented and their indication, advantages and restrictions are discussed with clinical relevance.

  11. Spatial imagery relies on a sensory independent, though sensory sensitive, functional organization within the parietal cortex: a fMRI study of angle discrimination in sighted and congenitally blind individuals.

    Science.gov (United States)

    Bonino, Daniela; Ricciardi, Emiliano; Bernardi, Giulio; Sani, Lorenzo; Gentili, Claudio; Vecchi, Tomaso; Pietrini, Pietro

    2015-02-01

    Although vision offers distinctive information to space representation, individuals who lack vision since birth often show perceptual and representational skills comparable to those found in sighted individuals. However, congenitally blind individuals may result in impaired spatial analysis, when engaging in 'visual' spatial features (e.g., perspective or angle representation) or complex spatial mental abilities. In the present study, we measured behavioral and brain responses using functional magnetic resonance imaging in sighted and congenitally blind individuals during spatial imagery based on a modified version of the mental clock task (e.g., angle discrimination) and a simple recognition control condition, as conveyed across distinct sensory modalities: visual (sighted individuals only), tactile and auditory. Blind individuals were significantly less accurate during the auditory task, but comparable-to-sighted during the tactile task. As expected, both groups showed common neural activations in intraparietal and superior parietal regions across visual and non-visual spatial perception and imagery conditions, indicating the more abstract, sensory independent functional organization of these cortical areas, a property that we named supramodality. At the same time, however, comparisons in brain responses and functional connectivity patterns across experimental conditions demonstrated also a functional lateralization, in a way that correlated with the distinct behavioral performance in blind and sighted individuals. Specifically, blind individuals relied more on right parietal regions, mainly in the tactile and less in the auditory spatial processing. In sighted, spatial representation across modalities relied more on left parietal regions. In conclusions, intraparietal and superior parietal regions subserve supramodal spatial representations in sighted and congenitally blind individuals. Differences in their recruitment across non-visual spatial processing in

  12. Characterization of the aspects of osteoprogenitor cell interactions with physical tetracalcium phosphate anchorage on titanium implant surfaces

    International Nuclear Information System (INIS)

    Well-designed implants are used not only to modify the geometry of the implant but also to change the chemical properties of its surfaces. The present study aims to assess the biofunctional effects of tetracalcium phosphate (TTCP) particles as a physical anchor on the implant surface derived through sandblasting. The characteristics of the surface, cell viability, and alkaline phosphatase (ALP) activity toward osteoprogenitor cells (D1) were obtained. D1 cells were cultured on a plain surface that underwent sandblasting and acid etching (SLA) (control SLA group) and on different SLA surfaces with different anchoring TTCP rates (new test groups, M and H). The mean anchoring rates were 57% (M) and 74% (H), and the anchored thickness was estimated to range from 12.6 μm to 18.3 μm. Compared with the control SLA surface on Ti substrate, the new test groups with different TTCP anchoring rates (M and H) failed to improve cell proliferation significantly but had a well-differentiated D1 cell phenotype that enhanced ALP expression in the early stage of cell cultures, specifically, at day 7. Results suggest that the SLA surface with anchored TTCP can accelerate progenitor bone cell mineralization. This study shows the potential clinical application of the constructed geometry in TTCP anchorage on Ti for dental implant surface modification. - Highlights: • TTCP (tetracalcium phosphate) as a physical anchorage on implant is characterized. • Theoretical values of anchored thickness and capping areas were estimated. • TTCP anchored by sandblasting can accelerate progenitor bone cell mineralization. • TTCP anchored on SLA (sandblasting and acid etching) surface is a promising method

  13. Characterization of the aspects of osteoprogenitor cell interactions with physical tetracalcium phosphate anchorage on titanium implant surfaces

    Energy Technology Data Exchange (ETDEWEB)

    Ko, Chia-Ling [Advanced Medical Devices and Composites Laboratory, Department of Fiber and Composite Materials, Feng Chia University, Taichung 40724, Taiwan (China); Dental Medical Devices and Materials Research Center, College of Dental Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan (China); Chang, Ya-Yuan; Liou, Cian-Hua [Alliance Global Technology Co., Ltd., Kaohsiung Medical Device Special Zone in Southern Taiwan Science Park, Kaohsiung 82151, Taiwan (China); Chen, Wen-Cheng, E-mail: wencchen@fcu.edu.tw [Advanced Medical Devices and Composites Laboratory, Department of Fiber and Composite Materials, Feng Chia University, Taichung 40724, Taiwan (China)

    2015-04-01

    Well-designed implants are used not only to modify the geometry of the implant but also to change the chemical properties of its surfaces. The present study aims to assess the biofunctional effects of tetracalcium phosphate (TTCP) particles as a physical anchor on the implant surface derived through sandblasting. The characteristics of the surface, cell viability, and alkaline phosphatase (ALP) activity toward osteoprogenitor cells (D1) were obtained. D1 cells were cultured on a plain surface that underwent sandblasting and acid etching (SLA) (control SLA group) and on different SLA surfaces with different anchoring TTCP rates (new test groups, M and H). The mean anchoring rates were 57% (M) and 74% (H), and the anchored thickness was estimated to range from 12.6 μm to 18.3 μm. Compared with the control SLA surface on Ti substrate, the new test groups with different TTCP anchoring rates (M and H) failed to improve cell proliferation significantly but had a well-differentiated D1 cell phenotype that enhanced ALP expression in the early stage of cell cultures, specifically, at day 7. Results suggest that the SLA surface with anchored TTCP can accelerate progenitor bone cell mineralization. This study shows the potential clinical application of the constructed geometry in TTCP anchorage on Ti for dental implant surface modification. - Highlights: • TTCP (tetracalcium phosphate) as a physical anchorage on implant is characterized. • Theoretical values of anchored thickness and capping areas were estimated. • TTCP anchored by sandblasting can accelerate progenitor bone cell mineralization. • TTCP anchored on SLA (sandblasting and acid etching) surface is a promising method.

  14. On Background Independence

    OpenAIRE

    Anderson, Edward

    2013-01-01

    This paper concerns what Background Independence itself is (as opposed to some particular physical theory that is background independent). The notions presented mostly arose from a layer-by-layer analysis of the facets of the Problem of Time in Quantum Gravity. Part of this coincides with two relational postulates which are thus identified as classical precursors of two of the facets of the Problem of Time. These are furthemore tied to the forms of each of the GR Hamiltonian and momentum cons...

  15. Securing financial independence in the legal basis of a central bank

    NARCIS (Netherlands)

    F. Amtenbrink (Fabian)

    2005-01-01

    textabstractTogether with institutional, functional and organizational independence, financial independence constitutes one of the cornerstones of central bank independence, the economic ratio of which has been demonstrated extensively in the literature. In broad terms financial independence may be

  16. Mechanical behavior experiment of wedge-bond anchorage for CFRP-tendons%CFRP筋复合型锚具的受力性能试验

    Institute of Scientific and Technical Information of China (English)

    王军; 李明君; 蔡东升; 刘德鑫

    2012-01-01

    In order to investigate the influence of main design parameters on anchorage performance and internal stress distribution of wedge-bond anchorage for CFRP tendons, five groups of parameter tests on wedge-bond anchorage were designed. The ultimate load, slip deformation of anchor ring and stress of metal sleeve stations were determined. The influence of design parameters on anchorage performance, the tress distribution and variation of metal sleeve were contrastively investigated through static loading testing. The results show that the anchorage performance with metal sleeve thickness of 2 mm, a wedge length of 65 mm, bond length of 500 mm is optimal. The anchor between wedge and metal sleeve is good without relative displacement. The tress distribution and variation of metal sleeve can reflect the internal stress distribution of anchorage, and the perk of stress curve indicates bonding failure between colloid and CFRP tendon near the observation point.%为研究CFRP筋复合型锚具的主要设计参数对锚固性能的影响及锚具内部受力情况,设计了5个复合型锚具的参数静载试验,测量了锚具的极限荷载、锚环-CFRP筋间滑移变形和金属筒测点应变,通过静载试验对比分析了各设计参数对锚固效果的影响,以及金属筒表面应力的分布情况与变化规律.研究结果表明:当金属筒壁厚为2 mm,夹片夹持长度为65 mm,粘结长度为500 mm时,锚具的锚固性能较好;夹片-金属筒间锚固良好,无滑移;金属筒应力分布与变化可反映锚具内部受力情况,测点应力曲线峰值预示该位置附近的胶体与CFRP筋间的粘结破坏.

  17. Independent technical review, handbook

    International Nuclear Information System (INIS)

    Purpose Provide an independent engineering review of the major projects being funded by the Department of Energy, Office of Environmental Restoration and Waste Management. The independent engineering review will address questions of whether the engineering practice is sufficiently developed to a point where a major project can be executed without significant technical problems. The independent review will focus on questions related to: (1) Adequacy of development of the technical base of understanding; (2) Status of development and availability of technology among the various alternatives; (3) Status and availability of the industrial infrastructure to support project design, equipment fabrication, facility construction, and process and program/project operation; (4) Adequacy of the design effort to provide a sound foundation to support execution of project; (5) Ability of the organization to fully integrate the system, and direct, manage, and control the execution of a complex major project

  18. Independent technical review, handbook

    Energy Technology Data Exchange (ETDEWEB)

    1994-02-01

    Purpose Provide an independent engineering review of the major projects being funded by the Department of Energy, Office of Environmental Restoration and Waste Management. The independent engineering review will address questions of whether the engineering practice is sufficiently developed to a point where a major project can be executed without significant technical problems. The independent review will focus on questions related to: (1) Adequacy of development of the technical base of understanding; (2) Status of development and availability of technology among the various alternatives; (3) Status and availability of the industrial infrastructure to support project design, equipment fabrication, facility construction, and process and program/project operation; (4) Adequacy of the design effort to provide a sound foundation to support execution of project; (5) Ability of the organization to fully integrate the system, and direct, manage, and control the execution of a complex major project.

  19. The Critical Independence Number and an Independence Decomposition

    CERN Document Server

    Larson, Craig Eric

    2009-01-01

    An independent set $I_c$ is a \\textit{critical independent set} if $|I_c| - |N(I_c)| \\geq |J| - |N(J)|$, for any independent set $J$. The \\textit{critical independence number} of a graph is the cardinality of a maximum critical independent set. This number is a lower bound for the independence number and can be computed in polynomial-time. Any graph can be decomposed into two subgraphs where the independence number of one subgraph equals its critical independence number, where the critical independence number of the other subgraph is zero, and where the sum of the independence numbers of the subgraphs is the independence number of the graph. A proof of a conjecture of Graffiti.pc yields a new characterization of K\\"{o}nig-Egervary graphs: these are exactly the graphs whose independence and critical independence numbers are equal.

  20. A maternal high fat diet programmes endothelial function and cardiovascular status in adult male offspring independent of body weight, which is reversed by maternal conjugated linoleic acid (CLA supplementation.

    Directory of Open Access Journals (Sweden)

    Clint Gray

    Full Text Available Maternal high fat intake during pregnancy and lactation can result in obesity and adverse cardio-metabolic status in offspring independent of postnatal diet. While it is clear that maternal high fat intake can cause hypertension in adult offspring, there is little evidence regarding the role of dietary interventions in terms of reversing these adverse effects. Conjugated linoleic acid (CLA is an omega 6 fatty acid with beneficial effects in obesity and metabolic status. However, the impact of CLA supplementation in the context of pregnancy disorders and high fat diet-induced developmental programming of offspring cardio-metabolic dysfunction has not been investigated. We have utilised a model of maternal overnutrition to examine the effects of CLA supplementation on programmed endothelial dysfunction during adulthood. Female Sprague-Dawley rats were fed either a purified control diet (CON or purified control diet supplemented with 1% CLA (of total fat, a purified high fat (HF diet (45%kcal from fat and a purified HF diet supplemented with 1% CLA (of total fat (HFCLA. All dams were fed ad libitum throughout pregnancy and lactation. Offspring were fed a standard chow diet from weaning (day 21 until the end of the study (day 150. Systolic blood pressure (SBP was measured at day 85 and 130 by tail cuff plethysmography. At day 150, offspring mesenteric vessels were mounted on a pressure myograph and vascular responses to agonist-induced constriction and endothelium-dependent vasodilators were investigated. SBP was increased at day 85 and 130 in HF and HFCLA adult male offspring compared to CON and CLA groups with no effect of CLA supplementation. An overall effect of a maternal HF diet was observed in adult male vessels with a reduced vasoconstrictor response to phenylephrine and blunted vasodilatory response to acetylcholine (ACh. Furthermore, HF and HFCLA offspring displayed a reduction in nitric oxide pathway function and an increased compensatory

  1. O envelhecimento ativo sob o olhar de idosos funcionalmente independentes El envejecimiento activo bajo la visión de ancianos funcionalmente independientes Active aging from the perspective of aged individuals who are functionally independent

    Directory of Open Access Journals (Sweden)

    Olívia Galvão Lucena Ferreira

    2010-12-01

    -structured interviews were performed with 100 functionally independent aged individuals from João Pessoa, Paraíba, Brazil. The data was organized and analyzed using Alceste software. Results showed that the aged individuals' statements about active aging are permeated with positive contents. However, when aging is not associated with the word active, it is still represented as losses and disabilities. Despite the existence of losses during the process, active aging should be encouraged among the elderly, as it means living a quality, plentiful life. Maintaining the elderly functionally independent is the first step to achieving active aging and thus improving their quality of life.

  2. Independent predictors of reliability between full time employee-dependent acquisition of functional outcomes compared to non-full time employee-dependent methodologies: a prospective single institutional study

    Science.gov (United States)

    Elsamadicy, Aladine A.; Cheng, Joseph; Bagley, Carlos

    2016-01-01

    Background The prospective acquisition of reliable patient-reported outcomes (PROs) measures demonstrating the effectiveness of spine surgery, or lack thereof, remains a challenge. The aims of this study are to compare the reliability of functional outcomes metrics obtained using full time employee (FTE) vs. non-FTE-dependent methodologies and to determine the independent predictors of response reliability using non FTE-dependent methodologies. Methods One hundred and nineteen adult patients (male: 65, female: 54) undergoing one- and two-level lumbar fusions at Duke University Medical Center were enrolled in this prospective study. Enrollment criteria included available demographic, clinical and baseline functional outcomes data. All patients were administered two similar sets of baseline questionnaires—(I) phone interviews (FTE-dependent) and (II) hardcopy in clinic (patient self-survey, non-FTE-dependent). All patients had at least a two-week washout period between phone interviews and in-clinic self-surveys to minimize effect of recall. Questionnaires included Oswestry disability index (ODI) and Visual Analog Back and Leg Pain Scale (VAS-BP/LP). Reliability was assessed by the degree to which patient responses to baseline questionnaires differed between both time points. Results About 26.89% had a history an anxiety disorder and 28.57% reported a history of depression. At least 97.47% of patients had a High School Diploma or GED, with 49.57% attaining a 4-year college degree or post-graduate degree. 29.94% reported full-time employment and 14.28% were on disability. There was a very high correlation between baseline PRO’s data captured between FTE-dependent compared to non-FTE-dependent methodologies (r=0.89). In a multivariate logistic regression model, the absence of anxiety and depression, higher levels of education (college or greater) and full-time employment, were independently associated with high response reliability using non

  3. Distro’: Independent Creativity for Independent Industr

    Directory of Open Access Journals (Sweden)

    Wiwik Sri Wulandari

    2014-11-01

    Full Text Available To shortened this introduction, ‘Distro’ is one of cultural phenomenon in theyoung generation nowadays. The word of ‘Distro’ is the shortened of DistributionOutlet. The phenomenon of ‘Distro’ has been some kind of new trends inproducing and distributing creative design products of goods amongst theyoungsters independently, in an independence industry that open for challengingand competitiveness for everyone. This field research has been done in the city ofYogyakarta, reknown as the second city in creative design products after the cityof Bandung. Yogyakarta is welknown as the students’ city as well as the capital cityof culture of Indonesia. As a students’ city it is normal that Yogyakarta is growingin numbers of young people who pursued to study here and enriched the cultureof the city to become more multicultural and the varieties of pluralism as well.This sociocultural phenomenon not only brought some dynamic changing tosociety, economy and cultural life of the city, but also social problems that needsto be overcome. My first research question then is about how the existence of‘Distro’ in Yogyakarta can be a positive answer for social problems that may arisesfrom the hegemony of globalization markets domestically? My second questionis how the creative product designs are being made and distributed creatively inindependent industry? Lastly, my third question is dealling with the genres ofthe design products and how it can be a new trend in art expression? ‘Distro’ is aproduct of culture and it is also creating cultural change in some aspects of the lifeof the youngsters who are ‘Distro’ enthusiasts. ‘Distro’ phenomenon basically is anoffensive to the hegemony of internationally branded product design which turnsto become more over-dominated to the domestic markets and industry and thus,‘Distro’ has the spirit of survival whilts at the same time producing opportunity ofenterpreneurship

  4. Model-independent differences

    DEFF Research Database (Denmark)

    Könemann, Patrick

    2009-01-01

    is fundamentally different. This paper reports on our ongoing work on model-independent diffs, i.e. a diff that does not directly refer to the models it was created from. Based on that, we present an idea of how the diff could be generalized, e.g. many atomic diffs are merged to a new, generalized diff. One use...

  5. Independent safety organization

    International Nuclear Information System (INIS)

    Brookhaven National Laboratory has conducted a study on the need and feasibility of an independent organization to investigate significant safety events for the Office for Analysis and Evaluation of Operational Data, USNRC. The study consists of three parts: the need for an independent organization to investigate significant safety events, alternative organizations to conduct investigations, and legislative requirements. The determination of need was investigated by reviewing current NRC investigation practices, comparing aviation and nuclear industry practices, and interviewing a spectrum of representatives from the nuclear industry, the regulatory agency, and the public sector. The advantages and disadvantages of alternative independent organizations were studied, namely, an Office of Nuclear Safety headed by a director reporting to the Executive Director for Operations (EDO) of NRC; an Office of Nuclear Safety headed by a director reporting to the NRC Commissioners; a multi-member NTSB-type Nuclear Safety Board independent of the NRC. The costs associated with operating a Nuclear Safety Board were also included in the study. The legislative requirements, both new authority and changes to the existing NRC legislative authority, were studied. 134 references

  6. Model-Independent Diffs

    DEFF Research Database (Denmark)

    Könemann, Patrick

    just contain a list of strings, one for each line, whereas the structure of models is defined by their meta models. There are tools available which are able to compute the diff between two models, e.g. RSA or EMF Compare. However, their diff is not model-independent, i.e. it refers to the models...

  7. Independence, Disengagement, and Discipline

    Science.gov (United States)

    Rubin, Ron

    2012-01-01

    School disengagement is linked to a lack of opportunities for students to fulfill their needs for independence and self-determination. Young people have little say about what, when, where, and how they will learn, the criteria used to assess their success, and the content of school and classroom rules. Traditional behavior management discourages…

  8. Bayesian Independent Component Analysis

    DEFF Research Database (Denmark)

    Winther, Ole; Petersen, Kaare Brandt

    2007-01-01

    In this paper we present an empirical Bayesian framework for independent component analysis. The framework provides estimates of the sources, the mixing matrix and the noise parameters, and is flexible with respect to choice of source prior and the number of sources and sensors. Inside the engine...

  9. Order Independence and Rationalizability

    NARCIS (Netherlands)

    Apt, K.R.

    2005-01-01

    Two natural strategy elimination procedures have been studied for strategic games. The first one involves the notion of (strict, weak, etc) dominance and the second the notion of rationalizability. In the case of dominance the criterion of order independence allowed us to clarify which notions and u

  10. Reduction of 99mTc-sestamibi and 99mTc-tetrofosmin uptake in MRP-expressing breast cancer cells under hypoxic conditions is independent of MRP function

    International Nuclear Information System (INIS)

    Hypoxia reduces the uptake of technetium-99m sestamibi (MIBI) in human cancer cell lines. In the current investigation, we attempted to identify the relationship between hypoxia-induced alteration of 99mTc-MIBI accumulation and expression of multi-drug resistance-associated protein (MRP) in the MCF7/WT breast cancer cell line and its subclonal cell line, MCF7/VP, which expresses high levels of MRP1. A second cationic compound, 99mTc-tetrofosmin (TF), was also examined. Cellular uptake of 99mTc-MIBI and 99mTc-TF was significantly higher in parental MCF7/WT cells than in MCF7/VP cells. Hypoxic conditions generated with a mixture of 95% N2 and 5% CO2 reduced cellular uptake of the two tracers in both parental MCF7/WT cells and MRP1-expressing MCF7/VP cells. Cell binding assay with iodine-125-labelled anti-MRP1 antibody demonstrated its specific binding to MCF7/VP cells. Hypoxia did not affect the amount of antibody bound to MCF7/VP cells. These results indicate that hypoxia-induced reduction of tracer uptake in tumour cells is a phenomenon independent of MRP function. (orig.)

  11. The Critical Independence Number and an Independence Decomposition

    OpenAIRE

    Larson, Craig Eric

    2009-01-01

    An independent set $I_c$ is a \\textit{critical independent set} if $|I_c| - |N(I_c)| \\geq |J| - |N(J)|$, for any independent set $J$. The \\textit{critical independence number} of a graph is the cardinality of a maximum critical independent set. This number is a lower bound for the independence number and can be computed in polynomial-time. Any graph can be decomposed into two subgraphs where the independence number of one subgraph equals its critical independence number, where the critical in...

  12. (Independência funcional na dependente relação de homens tetraplégicos com seus (insubstituíveis pais/cuidadores (Independencia funcional en la relación dependiente de hombres cuadripléjicos con sus (insustituibles padres/cuidadores Functional (independence in the dependent relationship of quadriplegic men with their (unreplaceable parents/caregivers

    Directory of Open Access Journals (Sweden)

    Wiliam César Alves Machado

    2010-03-01

    del discapacitado; tecnología de apoyo: soporte tecnológico para optimizar la calidad de los cuidados; miedos; futuro incierto y pérdida de los padres: los umbrales y las fragilidades humanas; y ganancias funcionales: respuestas objetivas de las funciones corporales. Se concluyó en que el apoyo familiar y, en particular, la presencia de los padres, son fundamentales para enfrentar las limitaciones y reaccionar en la búsqueda de equilibrios en la deficiencia, incapacidad, desventajas y salud de tales pacientes, preparándolos para alcanzar en forma gradual mejoras funcionales e independencia para realizar actividades diarias y ocuparse del autocuidado.The objective of this study was to identify elements of the international classification of functioning, disability and health, applicable to quadriplegic men's home care, to reduce the dependence on their parents' help for activities of daily living and self-care. Data were collected from June 2004 to March 2005. Semi-structured interviews were performed with eight adults with high spinal cord lesions who were being cared for at home . Content analysis was performed based on the categories of meaning extrapolated and the following themes were discovered : family support: safety for the corporal functions of the disabled; supporting technology: inventiveness to promote quality care; fears, uncertain future and parents' loss: thresholds and human fragilities; and functional gains: objective response of body functions. In conclusion, family support, and especially the presence of parents, is fundamental to facing limitations and to reacting in the search for balance with the deficiency, disability, disadvantage and health of that population, preparing them to achieve gradual functional gain and independence for daily activities and self-care.

  13. 新型锚固技术加固悬空岩体试验研究%Experiment Study on the New Anchorage Technology Reinforcing Suspended-rock

    Institute of Scientific and Technical Information of China (English)

    毛筱霏; 刘玉茜; 孙莹

    2011-01-01

    砂固预应力锚杆是一种新型的锚固技术,其锚固力是由砂体与锚杆孔壁之间的摩擦力和机械咬合力提供的,介绍了砂固预应力锚杆的构造形式及施工工艺,揭示了其工作机理,并对锚杆抗拔力进行了现场试验及理论研究,分析了锚杆抗拔力与位移的关系,以及砂体初始密度对抗拔力的影响.结果表明,此种锚杆具有优良的力学性能,完全可以满足小型岩体的加固要求,在工程支护领域中可以推广应用.%The sand-filled prestressed anchor is a new anchorage technology, and its anchorage force is actually the friction resistance and machine occlude force between sand and drill hole. The authors introduce the constitution and construction methods, and research the working mechanism of the sand-filling prestressed anchor. The anchor force test and theory of the anchor are studied. The relationship between anchorage force and displacement and the affection of original density of sand on anchorage force are analyzed. The test results indicate that the sand-filled prestressed anchor has excellent mechanics performance, and it can be satisfied the reinforced requests of minitype rock. The new technology can be widely used in supporting project.

  14. Factors influencing the anchorage strength of welded geogrid in soil%焊接土工格栅土中锚固强度的影响因素

    Institute of Scientific and Technical Information of China (English)

    徐超; 陈洪帅; 朱洪; 朱英俊; 石志龙

    2012-01-01

    A series of pull-out tests of 3 different selected types of welded geogrid buried in coarse grained soils are carried out in the present study. The anchorage strength and its influencing factors are analyzed based on the test results and according to the interaction mechanism of geogrid with soil. The results indicate that for geogrids with the same mesh size, the stronger the peel strength of a geogrid, the higher the anchorage strength in soils. The peel strength of welded geogrid is the controlling index, but not the only influencing factor to the geogrid anchorage strength in soil. The mesh size of georid and the property of soil are also of importance to the development of anchorage strength in soils.%选择3种不同规格的焊接土工格栅,完成了格栅在粗颗粒土中的一系列拉拔试验.根据试验结果和土工格栅与土的相互作用机理,分析了各种格栅在土中的锚固强度及其影响因素.结果表明:在网格尺寸相同的情况下,节点剥离强度越大,则土中锚固强度越高;焊接土工格栅的节点剥离强度是其土中锚固强度的控制指标,但非唯一影响因素,网格尺寸和填土性质也对格栅土中锚固阻力的发挥具有重要影响.

  15. Changes in strain patterns after implantation of a short stem with metaphyseal anchorage compared to a standard stem: an experimental study in synthetic bone

    Directory of Open Access Journals (Sweden)

    Jens Gronewold

    2014-03-01

    Full Text Available Short stem hip arthroplasties with predominantly metaphyseal fixation, such as the METHA® stem (Aesculap, Tuttlingen, Germany, are recommended because they are presumed to allow a more physiologic load transfer and thus a reduction of stress-shielding. However, the hypothesized metaphyseal anchorage associated with the aforementioned benefits still needs to be verified. Therefore, the METHA short stem and the Bicontact® standard stem (Aesculap, Tuttlingen, Germany were tested biomechanically in synthetic femora while strain gauges monitored their corresponding strain patterns. For the METHA stem, the strains in all tested locations including the region of the calcar (87% of the non-implanted femur were similar to conditions of synthetic bone without implanted stem. The Bicontact stem showed approximately the level of strain of the non-implanted femur on the lateral and medial aspect in the proximal diaphysis of the femur. On the anterior and posterior aspect of the proximal metaphysis the strains reached averages of 78% and 87% of the non-implanted femur, respectively. This study revealed primary metaphyseal anchorage of the METHA short stem, as opposed to a metaphyseal-diaphyseal anchorage of the Bicontact stem.

  16. 微种植体支抗在口腔正畸中的应用%Application of mini - implant anchorage in orthodontic treatment

    Institute of Scientific and Technical Information of China (English)

    侯亚男

    2011-01-01

    微种植体作为增强正畸支抗的措施获得了良好的临床效果.微种植体的植入手术简单,可提供绝对支抗,患者舒适感高.本文从微种植体的历史研究背景、作用原理、形态设计影响,植入方式和部位,临床应用,并发症及应用前景等方面进行详尽的综述.%With the rapid development of implant anchorage, the mini - implant has obtained good clinical results as one of the measures to enhance orthodontic anchorage, which has attracted wide attention. The procedure of implantation is simple, and after that the mini - implant can provide absolute anchorage and won't induce uncomfortableness for the patients. In this paper, we reviewed details of background, theory, effects of the design, style and position of the implantation, the clinical applications, the complications and the prospect.

  17. TO BE INDEPENDENT

    Institute of Scientific and Technical Information of China (English)

    石波

    2000-01-01

    @@ Ⅰ. Introduction At present, in the college, English extensive reading class, most students are not used to being independent. They always ask the teacher to explain the passages sentence by sentence and they need a lot of time to use the dictionary. Yet, we should take the responsibility for the students by making clear the difference between intensive and extensive reading. The traditional teaching approaches pays more attention to the teacher-centered way;the teacher always plays a monodrama, and the teacher dominates the class. The students are lack initiative. Some students do not know where they could start from, and the others are short of fast-reading skills, always fixing their eyes on one word or one sentence. Under the new situation and new thinking, students should learn to be more independent.

  18. Quantum independent increment processes

    CERN Document Server

    Franz, Uwe

    2005-01-01

    This volume is the first of two volumes containing the revised and completed notes lectures given at the school "Quantum Independent Increment Processes: Structure and Applications to Physics". This school was held at the Alfried-Krupp-Wissenschaftskolleg in Greifswald during the period March 9 – 22, 2003, and supported by the Volkswagen Foundation. The school gave an introduction to current research on quantum independent increment processes aimed at graduate students and non-specialists working in classical and quantum probability, operator algebras, and mathematical physics. The present first volume contains the following lectures: "Lévy Processes in Euclidean Spaces and Groups" by David Applebaum, "Locally Compact Quantum Groups" by Johan Kustermans, "Quantum Stochastic Analysis" by J. Martin Lindsay, and "Dilations, Cocycles and Product Systems" by B.V. Rajarama Bhat.

  19. Quantum independent increment processes

    CERN Document Server

    Franz, Uwe

    2006-01-01

    This is the second of two volumes containing the revised and completed notes of lectures given at the school "Quantum Independent Increment Processes: Structure and Applications to Physics". This school was held at the Alfried-Krupp-Wissenschaftskolleg in Greifswald in March, 2003, and supported by the Volkswagen Foundation. The school gave an introduction to current research on quantum independent increment processes aimed at graduate students and non-specialists working in classical and quantum probability, operator algebras, and mathematical physics. The present second volume contains the following lectures: "Random Walks on Finite Quantum Groups" by Uwe Franz and Rolf Gohm, "Quantum Markov Processes and Applications in Physics" by Burkhard Kümmerer, Classical and Free Infinite Divisibility and Lévy Processes" by Ole E. Barndorff-Nielsen, Steen Thorbjornsen, and "Lévy Processes on Quantum Groups and Dual Groups" by Uwe Franz.

  20. Do Independent Expert Directors Matter?

    OpenAIRE

    Masulis, Ronald; Ruzzier, Christian; Xiao, Sheng; ZHAO, SHAN

    2012-01-01

    The generally weak correlation between board independence and firm performance is a major empirical puzzle. One possible explanation is that director independence alone is not enough. To explore this possibility, we examine the full employment histories of independent directors at S&P 1500 companies. We define an independent expert director (IED) as an independent director who has worked in the same 2-digit SIC industry as the company where he/she serves as an independent director. We show th...

  1. Conditional Independence in Uncertainty Theories

    OpenAIRE

    Shenoy, Prakash P.

    2013-01-01

    This paper introduces the notions of independence and conditional independence in valuation-based systems (VBS). VBS is an axiomatic framework capable of representing many different uncertainty calculi. We define independence and conditional independence in terms of factorization of the joint valuation. The definitions of independence and conditional independence in VBS generalize the corresponding definitions in probability theory. Our definitions apply not only to probability theory, but al...

  2. Board independence and competence

    OpenAIRE

    Wagner, Alexander F

    2011-01-01

    This paper analyzes board independence and competence as distinct, but inextricably linked aspects of board effectiveness. Competent directors add shareholder value because they have better information about the quality of projects. While a CEO cares about shareholder value, he also wants his board to behave loyally to him by agreeing to projects that give him private benefits. Because many aspects of the CEO-board relationship are not contractible, the paper studies a model of relational con...

  3. International exploration by independents

    International Nuclear Information System (INIS)

    Recent industry trends indicate that the smaller U.S. independents are looking at foreign exploration opportunities as one of the alternatives for growth in the new age of exploration. The problems of communications and logistics caused by different cultures and by geographic distances must be carefully evaluated. A mid-term to long-term strategy tailored to the goals and the financial capabilities of the company should be prepared and followed by a careful planning of the operations. This paper addresses some aspects of foreign exploration that should be considered before an independent venture into the foreign field. It also provides some guidelines for conducting successful overseas operations. When properly assessed, foreign exploration is well within the reach of smaller U.S. independents and presents no greater risk than domestic exploration; the rewards, however, can be much larger. Furthermore, the Oil and Gas Journal surveys of the 300 largest U.S. petroleum companies show that companies with a consistent foreign exploration policy have fared better financially during difficult times

  4. Independent sets in hypergraphs

    CERN Document Server

    Balogh, József; Samotij, Wojciech

    2012-01-01

    Many important theorems in combinatorics, such as Szemer\\'edi's theorem on arithmetic progressions and the Erd\\H{o}s-Stone Theorem in extremal graph theory, can be phrased as statements about independent sets in uniform hypergraphs. In recent years, an important trend in the area has been to extend such classical results to the so-called sparse random setting. This line of research culminated recently in the breakthroughs of Conlon and Gowers and of Schacht, who developed general tools for solving problems of this type. In this paper, we provide a third, completely different approach to proving extremal and structural results in sparse random sets. We give a structural characterization of the independent sets in a large class of uniform hypergraphs by showing that every independent set is almost contained in one of a small number of relatively sparse sets. We then derive many interesting results as fairly straightforward consequences of this abstract theorem. In particular, we prove the well-known conjecture ...

  5. International exploration by independent

    International Nuclear Information System (INIS)

    Recent industry trends indicate that the smaller U.S. independents are looking at foreign exploration opportunities as one of the alternatives for growth in the new age of exploration. Foreign finding costs per barrel usually are accepted to be substantially lower than domestic costs because of the large reserve potential of international plays. To get involved in overseas exploration, however, requires the explorationist to adapt to different cultural, financial, legal, operational, and political conditions. Generally, foreign exploration proceeds at a slower pace than domestic exploration because concessions are granted by a country's government, or are explored in partnership with a national oil company. First, the explorationist must prepare a mid- to long-term strategy, tailored to the goals and the financial capabilities of the company; next, is an ongoing evaluation of quality prospects in various sedimentary basins, and careful planning and conduct of the operations. To successfully explore overseas also requires the presence of a minimum number of explorationists and engineers thoroughly familiar with the various exploratory and operational aspects of foreign work. Ideally, these team members will have had a considerable amount of on-site experience in various countries and climates. Independents best suited for foreign expansion are those who have been financially successful in domestic exploration. When properly approached, foreign exploration is well within the reach of smaller U.S. independents, and presents essentially no greater risk than domestic exploration; however, the reward can be much larger and can catapult the company into the 'big leagues.'

  6. International exploration by independents

    International Nuclear Information System (INIS)

    Recent industry trends indicate that the smaller US independents are looking at foreign exploration opportunities as one of the alternatives for growth in the new age of exploration. It is usually accepted that foreign finding costs per barrel are substantially lower than domestic because of the large reserve potential of international plays. To get involved overseas requires, however, an adaptation to different cultural, financial, legal, operational, and political conditions. Generally foreign exploration proceeds at a slower pace than domestic because concessions are granted by the government, or are explored in partnership with the national oil company. First, a mid- to long-term strategy, tailored to the goals and the financial capabilities of the company, must be prepared; it must be followed by an ongoing evaluation of quality prospects in various sedimentary basins, and a careful planning and conduct of the operations. To successfully explore overseas also requires the presence on the team of a minimum number of explorationists and engineers thoroughly familiar with the various exploratory and operational aspects of foreign work, having had a considerable amount of onsite experience in various geographical and climatic environments. Independents that are best suited for foreign expansion are those that have been financially successful domestically, and have a good discovery track record. When properly approached foreign exploration is well within the reach of smaller US independents and presents essentially no greater risk than domestic exploration; the reward, however, can be much larger and can catapult the company into the big leagues

  7. The lower bound on independence number

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    Let G be a graph with degree sequence (dv). If the maximum degree of any subgraph induced by a neighborhood of G is at most m, then the independence number of G is at least ∑vfm+1(dv), where fm+1(x) is a function greater than (log(x/(m+1))-1)/(x) for x>0. For a weighted graph G=(V,E,w), we prove that its weighted independence number (the maximum sum of the weights of an independent set in G) is at least ∑v(wv)/(1+dv), where wv is the weight of v.

  8. On-site tests to estimate aquifer permeability of drainage area of south caisson anchorage construction of Taizhou Bridge

    Institute of Scientific and Technical Information of China (English)

    Feng Zhaoxiang; Zhao Yanrong

    2011-01-01

    During the construction of the south caisson anchorage of Taizhou Bridge, the drainage area is located in the lower reaches of the Yangtze River and the permeability of stratums there is considerable. In order to maintain progress and guarantee safety during the sinking of the caisson, water should be drained in the initial period. Subsequently, detailed information about the aquifer permeability is required to make sure that the drainage will proceed successfully,which consequently necessitates the on-site estimation of the aquifer permeability in the drainage area. Therefore, the traditional pumping test and slug test are implemented respectively on site. The comparison of computational results of these two tests indicates that they are consistent overall. Notwithstanding, as slug test can be conducted with portable facilities in a short time and the manipulation is easy and few people need to be involved, the advantages of slug test is conspicuous compared with the traditional pumping test. It could be speculated that slug test will gain a prevalent application in the measurement of aquifer permeability in the future.

  9. 口腔正畸治疗中微型种植体支抗的使用价值%Value of micro- implant anchorage in orthodontic treatment

    Institute of Scientific and Technical Information of China (English)

    陈震春

    2011-01-01

    Objective To observe the success rate and effect of micro - implant anchorage in orthodontic treatment. Methods Totally 76 cases of needing orthodontic treatment patients, divided into experimental and control groups, experimental group using the orthodontic mini - implant anchorage method, while the control group using conventional methods of traditional orthodontics, to observe and compare their success rate and treatment. Results The test group and control group, no significant difference between general information, test group successfully planted micro - implant anchorage in 36 cases, the success rate was 97. 4% , growing successful anchorage stability is better, while the control group, 33 cases of successful a success rate of 84. 2% , lower than the experimental group, and the difference was statistically significant (P < 0. 05 ) , the experimental group molar shift less than the control group, the gap between the incisors protruding more than the control group, and the difference was statistically significant (P <0. 05). Conclusions Micro -implant anchorage than the traditional use of conventional methods with better efficacy, a higher success rate, is an ideal orthodontic treatment.%目的 观察微型种植体支抗在口腔正畸治疗中的成功率和效果.方法 选取76例需要正畸的患者,分为试验组和对照组,试验组采用微型种植体支抗的正畸方法,而对照组则采用常规的传统正畸方法,观察和对比其成功率和治疗效果.结果 试验组种植微型种植体支抗成功36例,成功率为97.4%,种植成功的支抗稳定性比较好,而对照组的成功33例,成功率为84.2%,比试验组低,且差异有统计学意义(P<0.05),试验组的磨牙移位比对照组少,上中切牙凸差距均比对照组多,差异有统计学意义(P<0.05).结论 微型种植体支抗的使用比传统的常规方法有着更好的疗效,成功率更高,是一种比较理想的正畸治疗方法.

  10. Oct4-induced oligodendrocyte progenitor cells enhance functional recovery in spinal cord injury model.

    Science.gov (United States)

    Kim, Jeong Beom; Lee, Hyunah; Araúzo-Bravo, Marcos J; Hwang, Kyujin; Nam, Donggyu; Park, Myung Rae; Zaehres, Holm; Park, Kook In; Lee, Seok-Jin

    2015-12-01

    The generation of patient-specific oligodendrocyte progenitor cells (OPCs) holds great potential as an expandable cell source for cell replacement therapy as well as drug screening in spinal cord injury or demyelinating diseases. Here, we demonstrate that induced OPCs (iOPCs) can be directly derived from adult mouse fibroblasts by Oct4-mediated direct reprogramming, using anchorage-independent growth to ensure high purity. Homogeneous iOPCs exhibit typical small-bipolar morphology, maintain their self-renewal capacity and OPC marker expression for more than 31 passages, share high similarity in the global gene expression profile to wild-type OPCs, and give rise to mature oligodendrocytes and astrocytes in vitro and in vivo. Notably, transplanted iOPCs contribute to functional recovery in a spinal cord injury (SCI) model without tumor formation. This study provides a simple strategy to generate functional self-renewing iOPCs and yields insights for the in-depth study of demyelination and regenerative medicine. PMID:26497893

  11. Functional role of eukaryotic translation initiation factor 4 gamma 1 (EIF4G1) in NSCLC

    Science.gov (United States)

    Cao, Yueyu; Wei, Mengdan; Li, Bing; Liu, Yali; Lu, Ying; Tang, Zhipeng; Lu, Tianbao; Yin, Yujiao; Qin, Zhiqiang; Xu, Zengguang

    2016-01-01

    Eukaryotic translation initiation factor 4 gamma 1(EIF4G1) is related to tumorigenesis and tumor progression. However, its role and the underlying mechanisms in the regulation of tumor development in non–small cell lung cancers (NSCLC) remain largely unknown. Here we report that the levels of EIF4G1 expression are much higher in NSCLC cell lines and tumor tissues than those in the normal lung cells and adjacent normal tissues from the same patients. Using shRNA to knock down EIF4G1 expression stably, we found EIF4G1 required for NSCLC cell proliferation, anchorage-independent growth, migration and invasion. Furthermore, silencing of EIF4G1 induces NSCLC cell apoptosis and causes G0/G1 cell cycle arrest. To identify the partner protein network of EIF4G1 in NSCLC cells, we found that Ubiquitin-specific protease 10 (USP10) can directly interacts with EIF4G1, while acting as a negative regulator for EIF4G1-mediated functions. Together, our results indicate that EIF4G1 functions as an oncoprotein during NSCLC development, which may represent a novel and promising therapeutic target in lung cancer. PMID:27003362

  12. The Membrane Protein of Severe Acute Respiratory Syndrome Coronavirus Functions as a Novel Cytosolic Pathogen-Associated Molecular Pattern To Promote Beta Interferon Induction via a Toll-Like-Receptor-Related TRAF3-Independent Mechanism

    Directory of Open Access Journals (Sweden)

    Yi Wang

    2016-02-01

    Full Text Available Most of the intracellular pattern recognition receptors (PRRs reside in either the endolysosome or the cytoplasm to sense pathogen-derived RNAs, DNAs, or synthetic analogs of double-stranded RNA (dsRNA, such as poly(I:C. However, it remains elusive whether or not a pathogen-derived protein can function as a cytosolic pathogen-associated molecular pattern (PAMP. In this study, we demonstrate that delivering the membrane gene of severe acute respiratory syndrome coronavirus (SARS-CoV into HEK293T, HEK293ET, and immobilized murine bone marrow-derived macrophage (J2-Mφ cells significantly upregulates beta interferon (IFN-β production. Both NF-κB and TBK1-IRF3 signaling cascades are activated by M gene products. M protein rather than M mRNA is responsible for M-mediated IFN-β induction that is preferentially associated with the activation of the Toll-like receptor (TLR adaptor proteins MyD88, TIRAP, and TICAM2 but not the RIG-I signaling cascade. Blocking the secretion of M protein by brefeldin A (BFA failed to reverse the M-mediated IFN-β induction. The antagonist of both TLR2 and TLR4 did not impede M-mediated IFN-β induction, indicating that the driving force for the activation of IFN-β production was generated from inside the cells. Inhibition of TRAF3 expression by specific small interfering RNA (siRNA did not prevent M-mediated IFN-β induction. SARS-CoV pseudovirus could induce IFN-β production in an M rather than M(V68A dependent manner, since the valine-to-alanine alteration at residue 68 in M protein markedly inhibited IFN-β production. Overall, our study indicates for the first time that a pathogen-derived protein is able to function as a cytosolic PAMP to stimulate type I interferon production by activating a noncanonical TLR signaling cascade in a TRAF3-independent manner.

  13. 脑卒中患者功能独立性与家庭照料者负担感的关系:以社会支持为中介变量和调节变量%The Relationship Between Stroke Patient's Functional Independence and Family Caregiver's Burden:Social Support As a Mediator and Moderator

    Institute of Scientific and Technical Information of China (English)

    王玉龙; 申继亮

    2012-01-01

    Many researchers believe that there is a significant relationship between patient's functional independence and family caregivers' burden. But in different family caregiving situations, the effect of a patient's functional independence on the family caregiver's burden is different. Researchers have found that social support is a very important variable to relieve stress, and social support can be separated into two categories according to different sources which are social support from patients and social support from non-patients. The present study aimed at investigating the relationship between functional independence and family caregiver's burden by adding 2 "third variables", namely, mediator and moderator. After summarizing the relevant reports, social support from the patient was considered as the mediator and social support from the non-patient as the moderator. 203 family caregivers of stroke patients were selected to fill 3 scales. The 3 scales used in this study were functional independence measure, social support questionnaire and caregiver strain index. Approximately 82.3% family caregivers of stroke patients felt stress apparently. There was a significantly negative correlation between the stroke patient's functional independence and the family caregiver's burden. Social support from stroke patients mediated the relationship between stroke patient's functional independence and family caregivers' burden, while social support from non-patient moderated the effect of stroke patient's functional independence on family caregiver's burden. Specifically, stroke patient's functional independence makes him (or her) act in concert with the caregiver, which would reduce family caregiver burden. This means that stroke patient's functional independence affects family caregiver's burden through increasing family caregiver's social support from patient. But social support from non-patient has a different mode of action and it

  14. CFRP预应力筋粘结式锚固系统的抗疲劳性能%Fatigue Behavior of Bond-type Anchorage with CFRP Tendon

    Institute of Scientific and Technical Information of China (English)

    方志; 龚畅; 杨剑; 孙志刚

    2012-01-01

    CFRP预应力筋锚固系统的系统研究成果尤其是疲劳性能研究仍较少,采用疲劳试验机对以高性能活性粉末混凝土RPC作为新型粘结介质的CFRP预应力筋粘结式锚具的疲劳性能进行试验研究,CFRP预应力筋锚固系统疲劳试验采取对组装件交替施加静荷载和疲劳荷载,即用静载试验来检验组装件在经历一定次数重复荷载后的静力性能变化.试验结果表明该类锚具具有良好的抗疲劳性能,随着循环加载次数的增加,组装件之间的相对位置将趋于更加稳定的状态;循环加载过程中CFRP筋抗拉刚度略有降低,疲劳136万次与疲劳前组装件CFRP筋的抗拉刚度比值为93.7%.循环荷载作用下对粘结式锚具组装件有损伤,但当所施加的荷载未超过其极限破断力的40%时,CFRP筋与RPC之间的相对位置将保持比较稳定的状态,此时存在一定损伤的粘结式锚具组装件仍具有较好的承载能力.%The research on the anchorage system for CFRP tendons, especially about the fatigue behavior, is weak. A series of fatigue tests were conducted for the developing a bond-type anchorage system for CFRP tendon characterized with RPC as bond medium by fatigue machine under static load and fatigue load alternatively. Then the static behavior of the anchorage system, which experienced certain cycling loading, was inspected by static load test. The test result shows that (1) the new type of anchorage system has good fatigue behavior, with the increasing of cycling load times, the relative position of the parts of anchorage system at more stable status; (2) the tensile stiffness of CFRP tendons be slightly lower in loading process, after 136 million fatigue tests, it is 93.7% of that before fatigue test; (3) harm of anchorage system will occur under cyclic loading, however the relative slip is smaller between CFRP tendon and RPC under less than 40% of ultimate load, and the higher bearing capacity of harmed

  15. The importance of board independence

    NARCIS (Netherlands)

    Zijl, N.J.M.

    2012-01-01

    Although the attributed importance of board independence is high, a clear definition of independence does not exist. Furthermore, the aim and consequences of independence are the subject of discussion and empirical evidence about the impact of independence is weak and disputable. Despite this lack o

  16. Role functioning before start of adjuvant treatment was an independent prognostic factor for survival and time to failure. A report from the Nordic adjuvant interferon trial for patients with high-risk melanoma

    DEFF Research Database (Denmark)

    Brandberg, Yvonne; Johansson, Hemming; Aamdal, Steinar;

    2013-01-01

    To investigate the role of health-related quality of life (HRQoL) at randomization as independent prognostic factors for survival and time to failure, and to explore associations between HRQoL and treatment effects....

  17. Neural maintenance roles for the matrix receptor dystroglycan and the nuclear anchorage complex in Caenorhabditis elegans

    NARCIS (Netherlands)

    Johnson, R.P.; Kramer, J.M.

    2012-01-01

    Recent studies in Caenorhabditis elegans have revealed specific neural maintenance mechanisms that protect soma and neurites against mispositioning due to displacement stresses, such as muscle contraction. We report that C. elegans dystroglycan (DG) DGN-1 functions to maintain the position of lumbar

  18. Joint cumulants for natural independence

    OpenAIRE

    Hasebe, Takahiro; Saigo, Hayato

    2011-01-01

    Many kinds of independence have been defined in non-commutative probability theory. Natural independence is an important class of independence; this class consists of five independences (tensor, free, Boolean, monotone and anti-monotone ones). In the present paper, a unified treatment of joint cumulants is introduced for natural independence. The way we define joint cumulants enables us not only to find the monotone joint cumulants but also to give a new characterization of joint cumulants fo...

  19. 采用独立分量分析观察经皮穴位电针刺激对脑功能的影响%Effects of Transcutaneous Electrical Acupoint Stimulation on Brain Function Explored with Independent Component Analysis

    Institute of Scientific and Technical Information of China (English)

    魏鹏绪; 卢虎英; 徐基民

    2007-01-01

    Objective To explore fMRI data with independent component analysis (ICA) in order to investigate effects of transcutaneous electrical acupoint stimulation (TEAS) on brain function. Methods The experiment was performed on a whole-body 1.5 T GE Signa Excite MRI scanner with which the brain oxygenation level dependent (BOLD)/EPI images were acquired from a female traumatic brain injury patient. A block designed protocol was used. Both durations of rest and TEAS were 30 seconds. The data processing was performed with GIFT, Statistical Parametric Mapping 5 (SPM5) and MRIcro. Results from ICA and SPM were compared. Results Extended Infomax algorithm provided by GIFT found thirteen independent components (ICs), each of which contained a spatial map and a corresponding time course. The spatial maps associated with task-related ICs resembled the activation maps from SPM5 but were not totally identical. In addition, the time courses of these ICs differed from the shape of canonical HRF model used by SPM. Conclusion ICA is a good choice to investigate data and obtain prior knowledge before using model-based methods such as SPM.%目的 使用独立分量分析方法探索督脉穴位经皮电刺激对脑功能的影响.方法 使用1.5T GE Signa Excite核磁成像仪对一位女性脑外伤患者进行BOLD成像.采用组块设计,静息期与刺激期交替,组块长度均为30 s.数据处理采用GIFT、SPM5和MRIcro软件进行,并将独立分量分析与SPM软件处理的结果进行比较.结果 采用GIFT中的扩展Infomax算法进行独立分量分析,显示有13个独立成分,每一独立成分包含一空间图和相应的时间变化曲线.任务相关性独立成分的空间激活图与SPM5的分析结果类似,但并不完全相同.此外,这些任务相关性独立成分的时间曲线与SPM所用的经典血流动力相应函数模型的形状并不一致.结论 在使用模型依赖的数据分析方法如SPM之前,可以使用独立分量分析探索fMRI数据并获得先验知识.

  20. Construction of Remuneration Mechanism with Stimulation Function for Independent Director%构建具有激励作用的独立董事薪酬机制研究

    Institute of Scientific and Technical Information of China (English)

    张晓明; 李辽宁; 李辉

    2011-01-01

    This article firstly analyzes the practice risks of independent director from four aspects which are closely related to the remuneration mechanism for independent director. These four aspects are law risk, reputation risk, market risk and economical risk. Secondly, this article analyzes the current situation and problems of the remuneration for independent director based on the statistics of remuneration for independent director from listed companies' annual reports for 2009. These listed companies are respectively located in Zhejiang province, Shaanxi province and Qinghai province. Finally we reconstruct the remuneration mechanism for independent director with three parts including fixedly payed remuneration, delayedly payed remuneration and stock notion.%从法律法规风险、声誉风险、市场风险和经济风险四个方面分析了与构建独立董事薪酬机制紧密相关的独立董事执业风险。对浙江、陕西、青海三省2009年上市公司独立董事薪酬的相关数据做了统计,分析了我国独立董事薪酬的现状以及存在的问题。重构了由固定薪酬、延期支付薪酬、股票期权构成的具有激励作用的独立董事薪酬机制。

  1. How Independent are Independent Directors? The Case of Italy

    OpenAIRE

    Paolo Santella; Giulia Paone; Carlo Drago

    2005-01-01

    In this article, we provide an interpretation for the voluntary independence requirements contained in the Italian Corporate Governance Code (Preda Code) checking them against a proxy for international best practice, the independence criteria provided in the EC Recommendation on non-executive and supervisory directors of 2005. We then check to what extent company disclosure for 2003 allows the verification of the independence of directors qualified as independent by the Italian 40 blue chips....

  2. A Component-Based Study of the Effect of Diameter on Bond and Anchorage Characteristics of Blind-Bolted Connections.

    Directory of Open Access Journals (Sweden)

    Muhammad Nasir Amin

    Full Text Available Structural hollow sections are gaining worldwide importance due to their structural and architectural advantages over open steel sections. The only obstacle to their use is their connection with other structural members. To overcome the obstacle of tightening the bolt from one side has given birth to the concept of blind bolts. Blind bolts, being the practical solution to the connection hindrance for the use of hollow and concrete filled hollow sections play a vital role. Flowdrill, the Huck High Strength Blind Bolt and the Lindapter Hollobolt are the well-known commercially available blind bolts. Although the development of blind bolts has largely resolved this issue, the use of structural hollow sections remains limited to shear resistance. Therefore, a new modified version of the blind bolt, known as the "Extended Hollo-Bolt" (EHB due to its enhanced capacity for bonding with concrete, can overcome the issue of low moment resistance capacity associated with blind-bolted connections. The load transfer mechanism of this recently developed blind bolt remains unclear, however. This study uses a parametric approach to characterising the EHB, using diameter as the variable parameter. Stiffness and load-carrying capacity were evaluated at two different bolt sizes. To investigate the load transfer mechanism, a component-based study of the bond and anchorage characteristics was performed by breaking down the EHB into its components. The results of the study provide insight into the load transfer mechanism of the blind bolt in question. The proposed component-based model was validated by a spring model, through which the stiffness of the EHB was compared to that of its components combined. The combined stiffness of the components was found to be roughly equivalent to that of the EHB as a whole, validating the use of this component-based approach.

  3. 优化复合锚固结构与单一锚固结构抗爆性能对比试验研究%Comparative test study of blast-resistance performance of optimal composite anchorage structure and single anchorage structure

    Institute of Scientific and Technical Information of China (English)

    赵林; 曾宪明; 李世民; 林大路; 陆卫国

    2011-01-01

    The optimal composite anchorage structure is constituted by soil nail and groups of fixed-length empty holes that regular setting in the end of soil nail. Empty holes caused a weakened zone in the surrounding rock. The weakened zone is between the reinforced support structure and the surround rock. It changes the original two-medium system into three-medium system. Under the explosion condition, weakened zone is firstly deformed, cracked, crushed or densified; and at the same time, a great deal of blast energy is absorbed. Therefore, the crisis of the reinforced support structure is transferred into the weakened zone. The optimal composite anchorage structure has excellent blast-resistance. The test results of the compression test of optimal composite anchorage structure and single anchorage structure show that the particle acceleration of single anchorage structure is 2.22 times higher than that of the optimal composite anchorage structure. The dynamic strain of the former is 5.30-4.50 times higher than that of the latter. The blast-resistance of the optimal composite anchorage structure is 5.10 times higher than that of the single anchorage structure. Under the limit damage condition, the former is 4.13-3.40 times higher than that of the latter.%优化复合锚固结构是由土钉和在土钉端部系统而规律地设置的一定长度的经特殊处理的空孔段群组成.空孔群在围岩介质中造成一个弱化区,弱化区介于支护结构与围岩介质之间,将原有的二介质系统(支护结构和围岩),改变为三介质系统(支护结构、弱化区和围岩).爆炸条件下,优先由弱化区产生变形、破碎、压实或压密,同时大量吸收爆炸能,使支护结构的危机得以转移至弱化区而本身不受损.优化复合锚固结构对比单一锚固结构具有优异的抗动静载性能.因此进行对比试验,研究优化复合锚固结构及单一锚固结构的抗爆性能.试验结果表明,在支护结构产生临

  4. Interpersonal leveling, independence, and self-enhancement

    DEFF Research Database (Denmark)

    Thomsen, Lotte; Sidanius, Jim; Fiske, Alan Page

    2007-01-01

    We argue that the relational model that people use for organizing specific social interactions in any culture determines whether people self-enhance. Self-enhancement is not a functional consequence of the (independent or interdependent) cultural model of self. Across three studies, Danes self-en...

  5. Product of n independent uniform random variables

    OpenAIRE

    Dettmann, Carl P.; Georgiou, Orestis

    2009-01-01

    Abstract We give an alternative proof of a useful formula for calculating the probability density function of the product of n uniform, independently and identically distributed random variables. Ishihara (2002, in Japanese) proves the result by induction; here we use Fourier analysis and contour integral methods which provide a more intuitive explanation of how the convolution theorem acts in this case. correspondance: Corresponding author. ...

  6. SHEAR LAG-DEBONDING MODEL FOR ANCHORAGE SECTION OF PRESTRESSED ANCHOR CABLE%预应力锚索锚固段的剪滞-脱黏模型

    Institute of Scientific and Technical Information of China (English)

    尤春安; 战玉宝; 刘秋媛; 孙琳琳; 王开彬

    2013-01-01

    Based on the calculating pattern of pulling prestressed anchorage body out from the base,the anchorage section can be divided into elastic region,plastic slide region and debonding region after analyzing the deformation and the damage process of the interface layer between anchorage body and grouting material,and the interfacial layer model for anchorage body and rock and soil mass is established.The theoretical solution of interfacial stress distribution was deduced by adopting the flow rule which associates with Coulomb condition.At the same time,a kind of new interface element model with properties of dilatancy and slippage is constructed; and interfacial stress distribution is calculated by two ways of numerical simulation and theoretical solution to verify the correctness of each other,so as to provide a new theoretical basis to calculate,analyze and design anchorage bodies.The results indicate that the main mechanical region is plastic slide region.So the plastic slippage state is the main state which the anchorage body usually in,so our researching and designing should be based on this state.In order to improve the anchoring force,we should improve maximum shear stress and extend the range of slip regime before the slip regime entering the debonding state.%基于预应力锚索锚固体从岩土体中拔出计算模式,通过锚固体与灌浆材料的界面界面层变形-破坏过程分析,将锚固段分为弹性区、塑性滑移区和脱黏区,建立锚固体-岩土体界面力学模型,采用与Coulomb条件关连的流动法则,导出界面应力分布的理论解;同时,构造具有剪胀和滑移特性的新型有限元界面单元,采用数值模拟的方法与界面应力分布的理论解进行相互验证,为锚固体受力的计算、分析与锚固体的设计提供理论依据.分析表明,锚固体的弹性区和脱黏区的受力较小,主要受力部分是塑性滑移区.因此,锚固体荷载的主要工况是塑性滑移状态,在研究

  7. From independence to expansion and back again

    DEFF Research Database (Denmark)

    Christiani, Tobias Lybecker; Pagh, Rasmus; Thorup, Mikkel

    2015-01-01

    We consider the following fundamental problems: • Constructing k-independent hash functions with a spacetime tradeoff close to Siegel’s lower bound. • Constructing representations of unbalanced expander graphs having small size and allowing fast computation of the neighbor function. It is not hard...... representation). While the previously most efficient construction (Thorup, FOCS 2013) needed time quasipolynomial in Siegel’s lower bound, our time bound is just a logarithmic factor from the lower bound....

  8. Direct proofs of order independence

    OpenAIRE

    Apt, K. R.

    2011-01-01

    We establish a generic result concerning order independence of a dominance relation on finite games. It allows us to draw conclusions about order independence of various dominance relations in a direct and simple way.

  9. PPARγ-Independent Mechanism

    Directory of Open Access Journals (Sweden)

    Christopher M. Hogan

    2011-01-01

    Full Text Available Acute and chronic lung inflammation is associated with numerous important disease pathologies including asthma, chronic obstructive pulmonary disease and silicosis. Lung fibroblasts are a novel and important target of anti-inflammatory therapy, as they orchestrate, respond to, and amplify inflammatory cascades and are the key cell in the pathogenesis of lung fibrosis. Peroxisome proliferator-activated receptor gamma (PPARγ ligands are small molecules that induce anti-inflammatory responses in a variety of tissues. Here, we report for the first time that PPARγ ligands have potent anti-inflammatory effects on human lung fibroblasts. 2-cyano-3, 12-dioxoolean-1, 9-dien-28-oic acid (CDDO and 15-deoxy-Δ12,14-prostaglandin J2 (15d-PGJ2 inhibit production of the inflammatory mediators interleukin-6 (IL-6, monocyte chemoattractant protein-1 (MCP-1, COX-2, and prostaglandin (PGE2 in primary human lung fibroblasts stimulated with either IL-1β or silica. The anti-inflammatory properties of these molecules are not blocked by the PPARγ antagonist GW9662 and thus are largely PPARγ independent. However, they are dependent on the presence of an electrophilic carbon. CDDO and 15d-PGJ2, but not rosiglitazone, inhibited NF-κB activity. These results demonstrate that CDDO and 15d-PGJ2 are potent attenuators of proinflammatory responses in lung fibroblasts and suggest that these molecules should be explored as the basis for novel, targeted anti-inflammatory therapies in the lung and other organs.

  10. Gauge independence of transition amplitudes in quantum electrodynamics

    International Nuclear Information System (INIS)

    Gauge independence of transition amplitudes in quantum electrodynamics is proved in the framework of covariant quantum electrodynamics exploited by Yokoyama. A systematic law of gauge transformation is given for general Green functions in QED. Gauge independence of the wave-function renormalization constant Z2 is also discussed to some extent. (author)

  11. Board independence and CEO turnover

    OpenAIRE

    Laux, Volker

    2005-01-01

    It is widely believed that the ideal board in corporations is composed almost entirely of independent (outside) directors. In contrast, this paper shows that some lack of board independence can be in the interest of shareholders. This follows because a lack of board independence serves as a substitute for commitment. Boards that are dependent on the incumbent CEO adopt a less aggressive CEO replacement rule than independent boards. While this behavior is inefficient ex post, it has positive e...

  12. Studying the Independent School Library

    Science.gov (United States)

    Cahoy, Ellysa Stern; Williamson, Susan G.

    2008-01-01

    In 2005, the American Association of School Librarians' Independent Schools Section conducted a national survey of independent school libraries. This article analyzes the results of the survey, reporting specialized data and information regarding independent school library budgets, collections, services, facilities, and staffing. Additionally, the…

  13. Media independence and dividend policy

    DEFF Research Database (Denmark)

    Farooq, Omar; Dandoune, Salma

    2012-01-01

    independence and dividend policies in emerging markets. Using a dataset from twenty three emerging markets, we show a significantly negative relationship between dividend policies (payout ratio and decision to pay dividend) and media independence. We argue that independent media reduces information asymmetries...

  14. Clinical application of mini-implant anchorage in orthodontics%钛金属微种植体钉作为支抗的临床应用

    Institute of Scientific and Technical Information of China (English)

    陈亚刚; 王兴; 吴文文

    2011-01-01

    BACKGROUND: Mini-implant anchorage is widely used in orthodontic fie Id. because it is small, easy to implant, use and remove,reliable in therapeutic effect and low cost.OBJECTIVE: To study the clinical application and effect of mini-implant anchorage in orthodontics.METHODS: The cep halo metric analysis was performed in 38 patients to compare overbite, coverage, molar relationship and thedegree of anterior adductor before and after treatment.RESULTSAHD CONCLUSION: The total number of mini-implants was 106, and allthe mini-implant anchorages weresuccessfully implanted except 3 cases required secondary operation with 5 loosen implants and one patient with loss of 2implants required re-implantation. Mini-implant anchorages make the treatment process s imple and acceptable in clinicalapplication.%背景:微种植体支抗因其体积小,植入、使用和去除简单,疗效可靠且成本低廉在正畸领域得到了广泛的应用.目的:观察分析钛金属微种植体支抗在口腔正畸中的应用效果.方法:对38例微种植体患者的投影测量分析,比较治疗前后的覆牙合、覆盖、磨牙关系和前牙内收程度等.结果与结论:微螺钉种植体植入总数106枚,除3例患者因5枚种植体松动再次手术植入,1例患者因2枚种植体脱落拒绝再次植入而改用传统支抗外,其他病例均获得了满意的治疗效果,建立了正常的前牙覆牙合、覆盖关系,患者面型得到了明显的改善.微种植体矫治过程相对简单,患者易于接受.

  15. Thermal Pollution Mathematical Model. Volume 6: Verification of Three-Dimensional Free-Surface Model at Anclote Anchorage. [environment impact of thermal discharges from power plants

    Science.gov (United States)

    Lee, S. S.; Sengupta, S.; Tuann, S. Y.; Lee, C. R.

    1980-01-01

    The free-surface model presented is for tidal estuaries and coastal regions where ambient tidal forces play an important role in the dispersal of heated water. The model is time dependent, three dimensional, and can handle irregular bottom topography. The vertical stretching coordinate is adopted for better treatment of kinematic condition at the water surface. The results include surface elevation, velocity, and temperature. The model was verified at the Anclote Anchorage site of Florida Power Company. Two data bases at four tidal stages for winter and summer conditions were used to verify the model. Differences between measured and predicted temperatures are on an average of less than 1 C.

  16. Effects of the earthquake of March 27, 1964, on the Eklutna Hydroelectric Project, Anchorage, Alaska, with a section on television examination of earthquake damage to underground communication and electrical systems in Anchorage: Chapter A in The Alaska earthquake, March 27, 1964: effects on transportation, communications, and utilities

    Science.gov (United States)

    Logan, Malcolm H.; with a section on Television Examination of Earthquake Damage to Underground Communication and Electrical Systems in Anchorage by Burton, Lynn R.

    1967-01-01

    The March 27, 1964, Alaska earthquake and its associated aftershocks caused damage requiring several million dollars worth of repair to the Eklwtna Hydroelectric Project, 34 miles northeast of Anchorage. Electric service from the Eklutna powerplant was interrupted during the early phase of the March 27 earthquake, built was restored (intermittently) until May 9,1964, when the plant was closed for inspection and repair. Water for Eklutna project is transported from Eklutna Lake to the powerplant at tidewater on Knik Arm of Cook Inlet by an underwater intake connected to a 4.46-mile tunnel penstock. The primary damage caused by the earthquake was 1at the intake structure in Eklutna Lake. No damage to the power tunnel was observed. The piles-supported powerplant and appurtenant structures, Anchorage and Palmer substations, and the transmission lines suffered minor dammage. Most damage occurred to facilities constructed on un-consolidated sediments and overburden which densified and subsided during the earthquake. Structures built on bedrock experienced little or no damage. Underground communication and electrical systems in Anchorage were examined with a small-diameter television camera to locate damaged areas requiring repair. Most of the damage was concentrated at or near valley slopes. Those parts of the systems within the major slide areas of the city were destroyed.

  17. Calpain Activity Is Generally Elevated during Transformation but Has Oncogene-Specific Biological Functions

    Directory of Open Access Journals (Sweden)

    N.O. Carragher

    2004-01-01

    Full Text Available Several oncogene and tumor-suppressor gene products are known substrates for the calpain family of cysteine proteases, and calpain is required for transformation by v-src and tumor invasion. Thus, we have now addressed whether calpain is generally associated with transformation and how calpain contributes to oncogene function. Our results demonstrate that calpain activity is enhanced upon transformation induced by the v-Src, v-Jun, v-Myc, k-Ras, and v-Fos oncoproteins. Furthermore, elevated calpain activity commonly promotes focal adhesion remodelling, disruption of actin cytoskeleton, morphological transformation, and cell migration, although proteolysis of target substrates (such as focal adhesion kinase, talin, and spectrin is differently specified by individual oncoproteins. Interestingly, v-Fos differs from other common oncoproteins in not requiring calpain activity for actin/adhesion remodelling or migration of v-Fos transformed cells. However, anchorage-independent growth of all transformed cells is sensitive to calpain inhibition. In addition, elevated calpain activity contributes to oncogene-induced apoptosis associated with transformation by v-Myc. Taken together, these studies demonstrate that calpain activity is necessary for full cellular transformation induced by common oncoproteins, but has distinct roles in oncogenic events induced by individual transforming proteins. Thus, targeting calpain activity may represent a useful general strategy for interfering with activated protooncogenes in cancer cells.

  18. Tumor suppressor function of Syk in human MCF10A in vitro and normal mouse mammary epithelium in vivo.

    Directory of Open Access Journals (Sweden)

    You Me Sung

    Full Text Available The normal function of Syk in epithelium of the developing or adult breast is not known, however, Syk suppresses tumor growth, invasion, and metastasis in breast cancer cells. Here, we demonstrate that in the mouse mammary gland, loss of one Syk allele profoundly increases proliferation and ductal branching and invasion of epithelial cells through the mammary fat pad during puberty. Mammary carcinomas develop by one year. Syk also suppresses proliferation and invasion in vitro. siRNA or shRNA knockdown of Syk in MCF10A breast epithelial cells dramatically increased proliferation, anchorage independent growth, cellular motility, and invasion, with formation of functional, extracellular matrix-degrading invadopodia. Morphological and gene microarray analysis following Syk knockdown revealed a loss of luminal and differentiated epithelial features with epithelial to mesenchymal transition and a gain in invadopodial cell surface markers CD44, CD49F, and MMP14. These results support the role of Syk in limiting proliferation and invasion of epithelial cells during normal morphogenesis, and emphasize the critical role of Syk as a tumor suppressor for breast cancer. The question of breast cancer risk following systemic anti-Syk therapy is raised since only partial loss of Syk was sufficient to induce mammary carcinomas.

  19. 独立成份分析在径向基函数网中的应用研究%STUDY ON APPLICATION OF INDEPENDENT COMPONENT ANALYSIS TO RADIAL BASIS FUNCTION NETWORK

    Institute of Scientific and Technical Information of China (English)

    郭穗勋; 黄榕波

    2004-01-01

    提出应用独立成份分析(Independent Component Analysis,ICA)降低径向基函数网(RBFN)输入维数的方法,并讨论ICA降维方法对RBFN行为的影响.实验表明基于ICA的降维方法大大提高了RBFN的收敛速度,改善RBFN的行为.

  20. K-independent percolation on trees

    CERN Document Server

    Mathieu, Pierre

    2011-01-01

    Consider the class of k-independent bond, respectively site, percolations with parameter p on an infinite tree T. We derive bounds on p in terms of k and the branching number br(T) of T for both a.s. percolation and a.s. nonpercolation. The bounds are tight for the whole class, coincide for bond and site percolation and are continuous functions of br(T). This extends previous results by Lyons for the independent (k=0) case and by Bollobas & Balister for the 1-independent bond percolation case. Central to our argumentation are moment method and capacity estimates \\`a la Lyons supplemented by explicit percolation models inspired by the work of Bollobas & Balister. An indispensable tool is the minimality and explicit construction of Shearer's measure on the k-fuzz of Z.