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Sample records for ananas comosus al

  1. Pineapple [Ananas comosus (L.) Merr].

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    Gangopadhyay, Gaurab; Mukherjee, Kalyan K

    2015-01-01

    The efficacy of Agrobacterium-mediated pineapple transformation technique has been improved (mean percentage of transgenic micro-shoots regenerated from initial callus explants up to 20.6%) using a novel encapsulation-based, antibiotic selection procedure. The detailed protocol using a standard plant transformation vector (pCAMBIA1304) as reported in an 'elite' Indian variety (Queen) of pineapple [Ananas comosus (L.) Merr] can be applied to other varieties of pineapple for introgression of target genes.

  2. do abacaxi (Ananas comosus L. Merril

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    Francisco Luiz Gumes Lopes

    2007-01-01

    Full Text Available The membrane separation process is used to recover, concentrate and/or even purify macromolecules. The reduction of the permeate flux rate during the operation time is one of the most important limiting factors in that process. It is associated to some phenomena such as the concentration polarization, the adsorption and the fouling. The Ananas comosus L. Merril is a source of bromelain enzyme (EC 3.4.22.5 with high commercial value and it is not produced in Brazil. In this work, the aim was to study the parameters that cause the reduction of the permeate flow rate in the bromelain enzyme recovering process through tangential microfiltrations of Ananas comosus juice using plane membranes and hollow fiber. Results showed that, at neutral pH, the effects of protein adsorption decreased and that the increase in pressure contributed to the fouling and enzyme denaturing

  3. (ananas comosus l. en costa rica

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    Steven Brenes-Prendas

    2007-01-01

    Full Text Available Reconocimiento taxonómico de arvenses y descripción de su manejo, en cuatro fincas productoras de piña (Ananas comosus L. en Costa Rica. El estudio se realizó en el mes de marzo del 2006, en cuatro fincas productoras de piña ubicadas en tres provincias de Costa Rica. Se realizaron levantamientos de arvenses presentes en cada finca; se describen también las prácticas de manejo que se usan para el control de estas arvenses. Se encontraron 58 especies de arvenses distribuidas en 19 familias botánicas. Se analizó el uso de herbicidas y ciclos de aplicación utilizados para el control de malezas y desecación de residuos de cosecha. Se consideró urgente el desarrollo de alternativas para el control de arvenses y el manejo de los residuos de cosecha en piña.

  4. Floral visitors of Ananas comosus in Ghana: A preliminary assessment

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    Peter Kwapong

    2010-11-01

    Full Text Available Ananas comosus var comosus (L. Merr. is the third most important tropical fruit in the world production and the leading foreign exchange earner among fresh fruits exported from Ghana. A survey was conducted in pineapple farms in the Central region of Ghana to identify floral visitors and their activities on the flowers. Nectar concentration and energetics and effect of floral visitors on fruit production were determined. Fourteen species of butterflies and one ant species were the main insect floral visitors as well as four species of sunbirds. The mean nectar concentration was 23.3% (± 0.39, SE and pollination limitation did not significantly affect fruit yield (weight: p = 0.285; length: p = 0.056; width: p= 0.268. The study showed that butterflies, ants and sunbirds are the main floral visitors on A. comosus. However their visits did not results in pollination and fruit production was not affected in any way by floral visitation. Still, it was found that A. comosus provides an important nectar resource for its foragers. Even if pollination is not crucial in pineapple cultivation, it is still essential in pineapple breeding programs to promote genetic diversity and conservation.

  5. Phenylpropanoid Glycosides from the Leaves of Ananas comosus.

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    Chen, Wen-Hao; Huang, Xiao-Juan; Shu, Huo-Ming; Hui, Yang; Guo, Fei-Yan; Song, Xiao-Ping; Ji, Ming-Hui; Chen, Guang-Ying

    2015-12-01

    Two new phenylpropanoid glycosides, named β-D-(1-O-acetyl-3,6-O-diferuloyl) fructofuranosyl β-D-6'-O-acetylglucopyranoside (1) and β-D-(1-O-acetyl-3,6-O-diferuloyl) fructofuranosyl α-D-glucopyranoside (2), along with two known analogues (3-4) and four glycerides (5-8), were isolated from the EtOAc extract of the leaves of Ananas comosus. Their structures were elucidated on the basis of 1D- and 2D-NMR analyses, as well as HR-ESI-MS experiments. Compounds 1-4 showed significant antibacterial activities against Staphylococcus aureus and Escherichia coli.

  6. In Vitro Effect of Ananas Comosus Extract on Human Sperm

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    Mélany Uribe-Clavijo

    2012-06-01

    Full Text Available Spermicides has been used for birth control, but its chemical components are harmful for the vaginal epithelium, thus other studies have been done, given the possibility to use different extracts from plants as spermicidal agents that avoid vaginal tissue damages. The aim of this study was to evaluate the Ananas comosus extract as spermicidal. Semen samples were incubated with pineapple extract, and motility and viability was evaluated after contact. In addition the cytotoxic effect was evaluated using MTS assay on HeLa cells. Motility (p<0.001 and viability (p<0.001 decreased after treatment with 50% dilution. However, extract had a cytotoxic effect on epithelial cell line. In conclusion, despite the cytotoxic effect in the same concentrations that spermicidal activity was observed, further studies determining the fractions of the extract have spermicidal properties are necessary.

  7. Developing Single Nucleotide Polymorphism (SNP) markers for the identification of pineapple (Ananas comosus) germplasm

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    Pineapple (Ananas comosus [L.] Merr.) is the third most important tropical fruit in the world after banana and mango and a major agricultural commodity in Hawaii. As a crop with vegetative propagation, genetic redundancy is a major challenge for efficient genebank management and in breeding. Using E...

  8. In vivo antitumoral activity of stem pineapple (Ananas comosus) bromelain.

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    Báez, Roxana; Lopes, Miriam T; Salas, Carlos E; Hernández, Martha

    2007-10-01

    Stem bromelain (EC 3.4.22.32) is a major cysteine proteinase, isolated from pineapple ( Ananas comosus) stem. Its main medicinal use is recognized as digestive, in vaccine formulation, antitumoral and skin debrider for the treatment of burns. To verify the identity of the principle in stem fractions responsible for the antitumoral effect, we isolated bromelain to probe its pharmacological effects. The isolated bromelain was obtained from stems of adult pineapple plants by buffered aqueous extraction and cationic chromatography. The homogeneity of bromelain was confirmed by reverse phase HPLC, SDS-PAGE and N-terminal sequencing. The in vivo antitumoral/antileukemic activity was evaluated using the following panel of tumor lines: P-388 leukemia, sarcoma (S-37), Ehrlich ascitic tumor (EAT), Lewis lung carcinoma (LLC), MB-F10 melanoma and ADC-755 mammary adenocarcinoma. Intraperitoneal administration of bromelain (1, 12.5, 25 mg/kg), began 24 h after tumor cell inoculation in experiments in which 5-fluorouracil (5-FU, 20 mg/kg) was used as positive control. The antitumoral activity was assessed by the survival increase (% survival index) following various treatments. With the exception of MB-F10 melanoma, all other tumor-bearing animals had a significantly increased survival index after bromelain treatment. The largest increase ( approximately 318 %) was attained in mice bearing EAT ascites and receiving 12.5 mg/kg of bromelain. This antitumoral effect was superior to that of 5-FU, whose survival index was approximately 263 %, relative to the untreated control. Bromelain significantly reduced the number of lung metastasis induced by LLC transplantation, as observed with 5-FU. The antitumoral activity of bromelain against S-37 and EAT, which are tumor models sensitive to immune system mediators, and the unchanged tumor progression in the metastatic model suggests that the antimetastatic action results from a mechanism independent of the primary antitumoral effect.

  9. Viability in the production of a drug extracted from Ananas comosus by a flat membrane system

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    Francisco Luiz Gumes Lopes

    2012-06-01

    Full Text Available The aim of this work was to study the production of e bromelain from the Ananas comosus L. Merril, by determining the process conditions using flat membranes. The production system modeling generated a hyperbolical curve and the optimization by response surfaces showed an influence of the transmembrane pressure higher than the pH influence. The cost of the production of bromelain from A. comosus was estimated 9 to 13 times lower than Sigma's retail sales price and 6.5 to 8.5 times lower than when this enzyme was obtained through a liquid-liquid extraction, which showed the economical feasibility of the process.

  10. Evaluation of Antioxidant Properties of Phenolics Extracted from Ananas comosus L.

    OpenAIRE

    Adhikarimayum HARIPYAREE; Kshetrimayum GUNESHWOR; Maibam DAMAYANTI

    2010-01-01

    Phenolics were extracted from the fruit tissues of Ananas comosus L. var. queen, cv. �Meitei Keehom�, a variety of pineapple grown in Manipur, India, after skin peeling, purified and their antioxidant properties were analyzed. The antioxidant properties were assessed based on the ability of fruit phenolics in absolute methanol to scavenge DPPH, superoxide anion radicals and hydroxyl radicals and compared to antioxidant compounds like ascorbic acid and pyragallol. Pineapple fruit phenolics sca...

  11. Transcriptome Sequence Analysis of an Ornamental Plant, Ananas comosus var. bracteatus, Revealed the Potential Unigenes Involved in Terpenoid and Phenylpropanoid Biosynthesis

    OpenAIRE

    Jun Ma; Kanakala, S.; Yehua He; Junli Zhang; Xiaolan Zhong

    2015-01-01

    Background Ananas comosus var. bracteatus (Red Pineapple) is an important ornamental plant for its colorful leaves and decorative red fruits. Because of its complex genome, it is difficult to understand the molecular mechanisms involved in the growth and development. Thus high-throughput transcriptome sequencing of Ananas comosus var. bracteatus is necessary to generate large quantities of transcript sequences for the purpose of gene discovery and functional genomic studies. Results The Anana...

  12. Proliferation Potential of 18-Month-Old Callus of Ananas comosus L. cv. Moris

    OpenAIRE

    Silva,A.E.; Kadir, M.A.; Aziz, M A; S. Kadzimin

    2006-01-01

    Differential effect of plant growth regulators and additives in proliferation of 18-month-old calli of Ananas comosus L. cv. Moris were assessed in vitro. The proliferation of callus relied on the growth regulators and additives. Of the different auxins supplemented in the Murashige and Skoog (MS) media, 32.22 μM α-naphthaleneacetic acid (NAA) gave the highest mean fresh weight of callus (46.817 g). Medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) was inferior to NAA, while b-n...

  13. Use of honey associated with Ananas comosus (Bromelin) in the treatment of acute irritative cough

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    Peixoto, Décio Medeiros; Rizzo, José Angelo; Schor, Deborah; Silva, Almerinda Rêgo; de Oliveira, Dinaldo Cavalcanti; Solé, Dirceu; Sarinho, Emanuel

    2016-01-01

    Abstract Objective: To evaluate the immediate improvement rate of irritative cough in patients treated with the combination of Ananas comosus extract and honey (Bromelin®) compared with the use of honey alone (placebo group). Methods: Pragmatic, double-blind, randomized, parallel-group study with children aged between 2 and 15 years, with irritative cough for at least 24hours. The double-blind assessment of cough was through the number of observed coughing episodes and intensity score for a period of 10minutes of observation. The decrease of one point in the mean total score was considered as a therapeutic effect. Results: There was a reduction in coughing episodes in both groups, as well as in the cough score after 30minutes of drug or honey administration. The change in clinical score above two points, which could indicate marked improvement, occurred in five patients in the bromelin group and only in one in the placebo group, but without significant difference. There were no adverse events. Conclusions: The immediate improvement rate of irritative cough was similar in patients treated with combination of Ananas comosus extract and honey (Bromelin®) compared with the use of honey alone (placebo group). It is possible that honey has a therapeutic effect on mucus and cough characteristics (Clinical Trials: NCT01356693). PMID:27181342

  14. Developing single nucleotide polymorphism markers for the identification of pineapple (Ananas comosus) germplasm.

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    Zhou, Lin; Matsumoto, Tracie; Tan, Hua-Wei; Meinhardt, Lyndel W; Mischke, Sue; Wang, Boyi; Zhang, Dapeng

    2015-01-01

    Pineapple (Ananas comosus [L.] Merr.) is the third most important tropical fruit in the world after banana and mango. As a crop with vegetative propagation, genetic redundancy is a major challenge for efficient genebank management and in breeding. Using expressed sequence tag and nucleotide sequences from public databases, we developed 213 single nucleotide polymorphism (SNP) markers and validated 96 SNPs by genotyping the United States Department of Agriculture - Agricultural Research Service pineapple germplasm collection, maintained in Hilo, Hawaii. The validation resulted in designation of a set of 57 polymorphic SNP markers that revealed a high rate of duplicates in this pineapple collection. Twenty-four groups of duplicates were detected, encompassing 130 of the total 170 A cosmos accessions. The results show that somatic mutation has been the main source of intra-cultivar variations in pineapple. Multivariate clustering and a model-based population stratification suggest that the modern pineapple cultivars are comprised of progenies that are derived from different wild Ananas botanical varieties. Parentage analysis further revealed that both A. comosus var. bracteatus and A. comosus var. ananassoides are likely progenitors of pineapple cultivars. However, the traditional classification of cultivated pineapple into horticultural groups (e.g. 'Cayenne', 'Spanish', 'Queen') was not well supported by the present study. These SNP markers provide robust and universally comparable DNA fingerprints; thus, they can serve as an efficient genotyping tool to assist pineapple germplasm management, propagation of planting material, and pineapple cultivar protection. The high rate of genetic redundancy detected in this pineapple collection suggests the potential impact of applying this technology on other clonally propagated perennial crops.

  15. Physiological Characterization and Comparative Transcriptome Analysis of White and Green Leaves of Ananas comosus var. bracteatus.

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    Li, Xia; Kanakala, Surapathrudu; He, Yehua; Zhong, Xiaolan; Yu, Sanmiao; Li, Ruixue; Sun, Lingxia; Ma, Jun

    2017-01-01

    Leaf coloration is one of the most important and attractive characteristics of Ananas comosus var. bracteatus. The chimeric character is not stable during the in vitro tissue culturing. Many regenerated plants lost economic values for the loss of the chimeric character of leaves. In order to reveal the molecular mechanisms involved in the albino phenotype of the leaf cells, the physiological and transcriptional differences between complete white (CWh) and green (CGr) leaf cells of A. comosus var. bracteatus were analyzed. A total of 1,431 differentially expressed unigenes (DEGs) in CGr and CWh leaves were identified using RNA-seq. A comparison to the COG, GO and KEGG annotations revealed DEGs involved in chlorophyll biosynthesis, chloroplast development and photosynthesis. Furthermore, the measurement of main precursors of chlorophyll in the CWh leaves confirmed that the rate-limiting step in chlorophyll biosynthesis, and thus the cause of the albino phenotype of the white cells, was the conversion of pyrrole porphobilinogen (PBG) to uroporphyrinogen III (Uro III). The enzyme activity of porphobilinogen deaminase (PBGD) and uroporporphyrinogn III synthase (UROS), which catalyze the transition of PBG to Uro III, was significantly decreased in the CWh leaves. Our data showed the transcriptional differences between the CWh and CGr plants and characterized key steps in chlorophyll biosynthesis of the CWh leaves. These results contribute to our understanding of the mechanisms and regulation of pigment biosynthesis in the CWh leaf cells of A. comosus var. bracteatus.

  16. Cloning, localization and expression analysis of vacuolar sugar transporters in the CAM plant Ananas comosus (pineapple).

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    Antony, Edna; Taybi, Tahar; Courbot, Mikaël; Mugford, Sam T; Smith, J Andrew C; Borland, Anne M

    2008-01-01

    In photosynthetic tissues of the CAM plant pineapple (Ananas comosus), storage of soluble sugars in the central vacuole during the daytime and their remobilization at night is required to provide carbon skeletons for nocturnal CO(2) fixation. However, soluble sugars produced photosynthetically must also be exported to support growth processes in heterotrophic tissues. To begin to address how vacuolar sugar storage and assimilate partitioning are regulated in A. comosus, degenerate PCR and cDNA library screening were used to clone three candidate sugar transporters from the leaves of this species. Subcellular localization of the three transporters was investigated via expression of YFP-fusion proteins in tobacco epidermal cells and their co-localization with subcellular markers by confocal microscopy. Using this strategy, a putative hexose transporter (AcMST1) and a putative inositol transporter (AcINT1) were identified that both localized to the tonoplast, whereas a putative sucrose transporter (AcSUT1) was found to localize to prevacuolar compartments. A cDNA (AcMST2) with high similarity to a recently characterized tonoplast hexose transporter in Arabidopsis was also identified from an A. comosus fruit EST database. Analyses of transcript abundance indicated that AcMST1 was more highly expressed in fruits compared to leaves of A. comosus, whilst transcripts of AcINT1, AcSUT1, and AcMST2 were more abundant in leaves. Transcript abundance of AcINT1, the putative inositol transporter, showed day-night changes comparable to those of other CAM-related transcripts described in Mesembryanthemum crystallinum. The results are discussed in terms of the role of vacuolar sugar transporters in regulating carbon flow during the diel cycle in CAM plants.

  17. Proliferation Potential of 18-Month-Old Callus of Ananas comosus L. cv. Moris

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    A.E. De Silva

    2006-01-01

    Full Text Available Differential effect of plant growth regulators and additives in proliferation of 18-month-old calli of Ananas comosus L. cv. Moris were assessed in vitro. The proliferation of callus relied on the growth regulators and additives. Of the different auxins supplemented in the Murashige and Skoog (MS media, 32.22 μM α-naphthaleneacetic acid (NAA gave the highest mean fresh weight of callus (46.817 g. Medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D was inferior to NAA, while b-naphthoxy acetic acid (BNOA and p-chlorophenoxy acetic acid (4-CPA were not effective in proliferating 18-months old callus. Addition of casein hydrolysate and coconut water to NAA supplemented medium showed better proliferation and production of callus. However, in terms of callus production, NAA at 32.22 μM was economically better.

  18. Proliferation potential of 18-month-old callus of Ananas comosus L. cv. Moris.

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    De Silva, A E; Kadir, M A; Aziz, M A; Kadzimin, S

    2006-02-17

    Differential effect of plant growth regulators and additives in proliferation of 18-month-old calli of Ananas comosus L. cv. Moris were assessed in vitro. The proliferation of callus relied on the growth regulators and additives. Of the different auxins supplemented in the Murashige and Skoog (MS) media, 32.22 microM alpha-naphthaleneacetic acid (NAA) gave the highest mean fresh weight of callus (46.817 g). Medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) was inferior to NAA, while b-naphthoxy acetic acid (BNOA) and p-chlorophenoxy acetic acid (4-CPA) were not effective in proliferating 18-months old callus. Addition of casein hydrolysate and coconut water to NAA supplemented medium showed better proliferation and production of callus. However, in terms of callus production, NAA at 32.22 microM was economically better.

  19. Desenvolvimento de Cerveja Artesanal com Acerola (Malpighia emarginata DC e Abacaxi (Ananas comosus L. Merril

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    L. I. F. Pinto

    2015-12-01

    Full Text Available A cerveja é a bebida alcoólica mais consumida no mundo. Estando bem relacionado com festividades e comemorações, porém essa bebida contém uma variedade de compostos bioativos, vitaminas e minerais, capazes de promover benefícios quando a sua ingestão é feita de maneira moderada. O mercado está a cada dia buscando produtos diferenciados e até exclusivos, surge assim o campo das cervejas artesanais. O objetivo deste trabalho foi desenvolver uma cerveja artesanal com acerola e abacaxi, trazendo uma nova alternativa ao nicho de mercado, além da associação ao aumento de suas características funcionais. O experimento foi conduzido no Laboratório de Cereais, Universidade Federal do Ceará – UFC, onde a cerveja foi produzida em escala laboratorial passando pelos processos de moagem do malte, mosturação, cozimento, decantação, filtração, fermentação, envase, maturação e primming, sendo adicionada a polpa de fruta em percentagem definidas por DIC, pasteurização e armazenamento. As amostras foram submetidas à análise físico-química, microbiológica e sensorial. Os resultados foram submetidos ao teste de normalidade, ao teste de média tukey e a ANOVA pelo software STATISTIC 10. A utilização das polpas de abacaxi e acerola como adjuntos no processamento da cerveja mostrou-se uma alternativa viável demostrada pelos resultados satisfatórios na avaliação sensorial, além de características físico-químicas de uma cerveja artesanal ácida/frutada. Development of craftbeer with Acerola (Malpighia emarginata DC and pineapple (Ananas comosus L. MerrilAbstract: The beer is the most consumed alcoholic beverage in the world. It is well associated with holidays and celebrations, but this beverage contains a variety of bioactive compounds, vitamins and minerals that promote benefits when its intake is made moderate manner. The market is every day looking for different and even unique products, thus arises the field of craft

  20. Characteristics of the carcass of goats of different genotypes fed pineapple (Ananas comosus L. stubble hay

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    Rosângela Maria Brito Lima

    2015-02-01

    Full Text Available The effect of the substitution of Tifton hay (0, 33, 66, 100 g/100 g for pineapple (Ananas comosus L. stubble hay was evaluated in the characteristics of the carcasses of goats of an unknown breed (UB in feedlot. Thirty-two bucks with an average initial live weight of 17.5±1.3 kg, at approximately 150 days of age, were housed in individual stalls provided with feeding and drinking troughs. The animals were distributed in a completely randomized design with four treatments and eight replications. There was a quadratic effect on the weights of hot carcass and cold carcass, empty body, and loin eye area. A linear increase was observed for losses by carcass cooling. The weights of commercial cuts and the weights of total muscle, total bone, intramuscular fat, and total fat decreased linearly. All morphometric measurements were influenced by the inclusion of pineapple stubble hay in the .diets. Substitution of Tifton hay for pineapple stubble hay at the level of 33 g/100 g improves the carcasses of UB goats qualitatively and quantitatively

  1. Functional characterization of recombinant bromelain of Ananas comosus expressed in a prokaryotic system.

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    George, Susan; Bhasker, Salini; Madhav, Harish; Nair, Archana; Chinnamma, Mohankumar

    2014-02-01

    Bromelain (BRM) is a defense protein present in the fruit and stem of pineapple (Ananas comosus) and it is grouped as a cysteine protease enzyme with diversified medicinal uses. Based on its therapeutic applications, bromelain has got sufficient attention in pharmaceutical industries. In the present study, the full coding gene of bromelain in pineapple stem (1,093 bp) was amplified by RT-PCR. The PCR product was cloned, sequenced, and characterized. The sequence analysis of the gene revealed the single nucleotide polymorphism and its phylogenetic relatedness. The peptide sequence deduced from the gene showed the amino acid variations, physicochemical properties and secondary and tertiary structural features of the protein. The full BRM gene was transformed to prokaryotic vector pET32b and expressed in Escherichia coli BL21 DE3pLysS host cells successfully. The identity of the recombinant bromelain (rBRM) protein was confirmed by Western blot analysis using anti-BRM-rabbit IgG antibody. The activity of recombinant bromelain compared with purified native bromelain was determined by protease assay. The inhibitory effect of rBRM compared with native BRM in the growth of Gram-positive and Gram-negative strains of Streptococcus agalactiae and Escherichia coli O111 was evident from the antibacterial sensitivity test. To the best of our knowledge, this is the first report showing the bactericidal property of rBRM expressed in a prokaryotic system.

  2. Biosorption of Basic Green 4 from aqueous solution by Ananas comosus (pineapple) leaf powder.

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    Chowdhury, Shamik; Chakraborty, Sagnik; Saha, Papita

    2011-06-01

    Biosorption characteristics of Ananas comosus (pineapple) leaf powder was investigated for decolorization of Basic Green 4 (BG 4), a cationic dye from its aqueous solutions employing a batch experimental set-up. Parameters that influence the sorption process such as pH, biosorbent dosage, contact time, initial dye concentration and temperature were systematically studied. The optimum conditions for removal of BG 4 were found to be pH 9.0, contact time=150 min, biosorbent dosage=5.0 g L(-1), initial dye concentration=50 mg L(-1). The temperature had a strong influence on the biosorption process. Further, the biosorbent was characterized by Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM) and Brunauer, Emmett, Teller (BET) surface area and pore size analysis. Experimental biosorption data were modeled by Langmuir, Freundlich and Dubinin-Radushkevich (D-R) isotherms. The biosorption process followed the Langmuir isotherm model with high coefficients of correlation (R(2)>0.99) at different temperatures. The pseudo second order kinetic model fitted well in correlation to the experimental results. Activation energy of the biosorption process (E(a)) was found to be 45.79 kJ mol(-1) by using the Arrhenius equation, indicating chemisorption nature of BG 4 sorption onto pineapple leaf powder. Thermodynamic parameters suggest that the biosorption process is spontaneous and exothermic in nature. Overall, the present findings suggest that this environmentally friendly, efficient and low-cost biosorbent may be useful for the removal of BG 4 from aqueous media.

  3. Resonance assignments and secondary structure of a phytocystatin from Ananas comosus.

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    Irene, Deli; Chen, Bo-Jiun; Lo, Si-Hung; Liu, Ting-Hang; Tzen, Jason T-C; Chyan, Chia-Lin

    2012-04-01

    A cDNA encoding a cysteine protease inhibitor, cystatin was cloned from pineapple (Ananas comosus L.) stem. This clone was constructed into an expression vector and expressed in E. coli and purified to homogeneous. The recombinant pineapple cystatins (AcCYS) showed effectively inhibitory activity toward cysteine proteases including papain, bromelain, and cathepsin B. In order to unravel its inhibitory action from structural point of view, multidimensional heteronuclear NMR techniques were used to characterize the structure of AcCYS. The full (1)H, (15)N, and (13)C resonance assignments of AcCYS were determined. The secondary structure of AcCYS was identified by using the assigned chemical shift of (1)Hα, (13)Cα, (13)Cβ, and (13)CO through the consensus chemical shift index (CSI). The results of CSI analysis suggest 5 β-strands (residues 45-47, 84-91, 94-104, 106-117, and 123-130) and one α-helix (residues 55-73).

  4. EFFICIENT USE OF TEMPORARY IMMERSION BIOREACTOR (TIB ON PINEAPPLE (Ananas comosus L. MULTIPLICATION AND ROOTING ABILITY

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    Biruk Ayenew

    2013-02-01

    Full Text Available Pineapple (Ananas comosus L. is one of the most potential fruit crop growing in Ethiopia due to suitable agro-ecology and economic importance. However, it is difficult to meet the demand for planting materials using the conventional propagation techniques due to production inefficiency and disease transmission. The experiment is laid in Completely Randomized Design with three treatments of vessels used along its media type, Temporary Immersion Bioreactor, TIB (RITA®, Vitropic, France , Glass jam jar and plastic jars , replicated six times on MS medium. A highly significant difference (p<0.001 was observed between culturing vessels with the same media supplement. From this study, it was found that pineapple explants cultured on TIB having full strength MS media supplemented with 2 mgl-1BA and 30gl–1 sucrose was found to be better which gave an average multiplication of 13.17 shoots per explant within six weeks of culture. Similarly plantlets cultured on TIB with half strength MS media supplemented with 3mgl-1 IBA and 40 g l-1 sucrose developed on average 16.33 roots having 6.27 cm length with well developed hairy root in four weeks of culture period that performed better in acclimatization facility and open field too.

  5. Agrobacterium-mediated genetic transformation of pineapple (Ananas comosus L., Merr.).

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    Mhatre, Minal

    2013-01-01

    Pineapple (Ananas comosus L., Merr.) is a commercially important crop, grown in the tropical and subtropical regions. However, the crop is faced with postharvest damage and poor varietal and nutritional improvement. Being a vegetatively propagated crop, conventional breeding programs take longer time for genetic improvement, which may not necessarily successfully develop an improved cultivar. Hence, the genetic modification of pineapple is an alternative handy approach to improve pineapple. We have established an Agrobacterium-mediated transformation system using leaf bases from in vitro-grown pineapple plants. Being a monocot, acetosyringone is added to the culture medium for overnight growth of Agrobacterium and transformation to transfer a gene of interest MSI99 soybean ferritin. Leaf bases isolated from in vitro shoot cultures are treated with Agrobacterium suspension at two dilutions, 10× and 20×, for 30 min. Explants are subsequently blot dried and cultured on gelrite solidified hormone-free Pin1 medium for 2 days (cocultivation). Periodic transfer is first done to the regeneration medium (Pin1) containing cefotaxime for the suppression of Agrobacterium growth. The transformants are selected by culturing on Pin1 medium containing cefotaxime and kanamycin. Multiple shoots, regenerated in leaf bases, are further multiplied and individually rooted in the liquid RM medium amended with antibiotics to recover plants. Putative transformants are analyzed for transgene integration and expression using standard molecular biological methods of PCR, RT-PCR, and genomic Southern.

  6. Purification, characterization, and antifungal activity of chitinases from pineapple (Ananas comosus) leaf.

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    Taira, Toki; Toma, Noriko; Ishihara, Masanobu

    2005-01-01

    Three chitinases, designated pineapple leaf chitinase (PL Chi)-A, -B, and -C were purified from the leaves of pineapple (Ananas comosus) using chitin affinity column chromatography followed by several column chromatographies. PL Chi-A is a class III chitinase having a molecular mass of 25 kDa and an isoelectric point of 4.4. PL Chi-B and -C are class I chitinases having molecular masses of 33 kDa and 39 kDa and isoelectric points of 7.9 and 4.6 respectively. PL Chi-C is a glycoprotein and the others are simple proteins. The optimum pHs of PL Chi-A, -B, and -C toward glycolchitin are pH 3, 4, and 9 respectively. The chitin-binding ability of PL Chi-C is higher than that of PL Chi-B, and PL Chi-A has lower chitin-binding ability than the others. At low ionic strength, PL Chi-B exhibits strong antifungal activity toward Trichoderma viride but the others do not. At high ionic strength, PL Chi-B and -C exhibit strong and weak antifungal activity respectively. PL Chi-A does not have antifungal activity.

  7. Cultivar identification and genetic relationship of pineapple (Ananas comosus) cultivars using SSR markers.

    Science.gov (United States)

    Lin, Y S; Kuan, C S; Weng, I S; Tsai, C C

    2015-11-25

    The genetic relationships among 27 pineapple [Ananas comosus (L.) Merr.] cultivars and lines were examined using 16 simple sequence repeat (SSR) markers. The number of alleles per locus of the SSR markers ranged from 2 to 6 (average 3.19), for a total of 51 alleles. Similarity coefficients were calculated on the basis of 51 amplified bands. A dendrogram was created according to the 16 SSR markers by the unweighted pair-group method. The banding patterns obtained from the SSR primers allowed most of the cultivars and lines to be distinguished, with the exception of vegetative clones. According to the dendrogram, the 27 pineapple cultivars and lines were clustered into three main clusters and four individual clusters. As expected, the dendrogram showed that derived cultivars and lines are closely related to their parental cultivars; the genetic relationships between pineapple cultivars agree with the genealogy of their breeding history. In addition, the analysis showed that there is no obvious correlation between SSR markers and morphological characters. In conclusion, SSR analysis is an efficient method for pineapple cultivar identification and can offer valuable informative characters to identify pineapple cultivars in Taiwan.

  8. 16S ribosomal DNA characterization of nitrogen-fixing bacteria isolated from banana (Musa spp.) and pineapple (Ananas comosus (L.) Merril).

    Science.gov (United States)

    Magalhães Cruz, L; de Souza, E M; Weber, O B; Baldani, J I; Döbereiner, J; Pedrosa, F de O

    2001-05-01

    Nitrogen-fixing bacteria isolated from banana (Musa spp.) and pineapple (Ananas comosus (L.) Merril) were characterized by amplified 16S ribosomal DNA restriction analysis and 16S rRNA sequence analysis. Herbaspirillum seropedicae, Herbaspirillum rubrisubalbicans, Burkholderia brasilensis, and Burkholderia tropicalis were identified. Eight other types were placed in close proximity to these genera and other alpha and beta Proteobacteria.

  9. Utilization of the biorreactor of imersion by bubbles at the micropropagation of Ananas comosus L. Merril

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    Gessiel Newton Scheidt

    2009-11-01

    Full Text Available The research for new techniques of in vitro cultivation is being object of many studies around the world, in order to optimize and decrease production costs of seedlings with agronomical interest. The main goal of this work was to compare different systems of in vitro cultivations using Ananas comosus L. Merril. So, the in vitro growth of the plantlets was promoted in two different bioreactors: Bioreactor of Immersion by Bubbles (B.I.B.® and the Reactor of Temporary Immersion (R.I.T.A.® with immersion cycle every 2 hours for 15 minutes and the traditional system in flasks with 200 mL. All cultivation systems used the MS liquid nutritive solution, supplemented with BAP (1 mgL-1, ANA (0.25 mgL-1, sucrose (30 gL-1 and Tween 20® (0.5 µL. The pH was adjusted to 5.8 and sterilized at 120°C for 15 minutes. The cultures were kept into a growth room during 30 days, with controlled temperature of 25±2°C, under white cold light (46.8 µmol.m-2.s-1, with photoperiod of 16 hours. The experimental design used was randomized, with three treatments, three repetitions and ten plants each stage. Among the evaluated systems, the BIB® presented the best results for the tested variables, mainly the total number of shoots, being 2.3 e 3.1 times superior when compared with the system R.I.T.A.® and the traditional consecutively. So the system of immersion by bubbles turns into an effective equipment to produce seedlings of pineapple in large scale.A busca por novas técnicas de cultivo in vitro vem sendo amplamente estudadas, visando otimizar e baixar o custo de produção das mudas que tenham interesse agronômico. O objetivo deste trabalho foi comparar diferentes sistemas de cultivo in vitro de Ananas comosus L. Merril. Para tanto, o crescimento in vitro de plântulas foi promovido em sistemas de biorreatores (B.I.B.® e R.I.T.A.® com ciclo de imersão a cada 2 horas por 15 minutos e o sistema tradicional em frascos de 200 mL. Em todos os sistemas de

  10. Evaluation of Antioxidant Properties of Phenolics Extracted from Ananas comosus L.

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    Adhikarimayum HARIPYAREE

    2010-06-01

    Full Text Available Phenolics were extracted from the fruit tissues of Ananas comosus L. var. queen, cv. �Meitei Keehom�, a variety of pineapple grown in Manipur, India, after skin peeling, purified and their antioxidant properties were analyzed. The antioxidant properties were assessed based on the ability of fruit phenolics in absolute methanol to scavenge DPPH, superoxide anion radicals and hydroxyl radicals and compared to antioxidant compounds like ascorbic acid and pyragallol. Pineapple fruit phenolics scavenged DPPH, superioxide anion radicals and hydroxyl radicals in a dose dependent way. In DPPH assay, the IC50 values of pineapple phenolics, ascorbic acid and pyragallol were 12.2?g/ml, 17.82?g/ml and 15.92?g/ml respectively. In superoxide anion and hydroxyl radical scavenging activities, the IC50 values of pineapple phenolics were 11.42?g/ml and 55.292?g/ml, for ascorbic acid 49.62?g/ml, 48.52?g/ml and that of pyragallol was 15.672?g/ml and 60.62?g/ml. The IC50 value was lowest in pineapple phenolics than ascorbic acid and pyragallol in DPPH and superoxide anion assays. But it is higher than ascorbic acid and lower than pyragallol in hydroxyl radical assay. The lower the IC50 values, the higher the antioxidant activities. The phenolics extracted from this variety of pineapple exhibit excellent free radical scavenging activity. The result shows that pineapple and its active constituents may be used in further antioxidative therapy.

  11. Hypolipidemic mechanisms of Ananas comosus L. leaves in mice: different from fibrates but similar to statins.

    Science.gov (United States)

    Xie, Weidong; Wang, Wei; Su, Hui; Xing, Dongming; Cai, Guoping; Du, Lijun

    2007-03-01

    In this study, we investigated hypolipidemic mechanisms of the ethanolic extract of Ananas comosus L. leaves (AC) in mice and then determined its activities in related enzymes. The results showed that AC (0.40 g/kg) significantly inhibited the increase in serum triglycerides by 40% in fructose-fed mice. In mice induced by alloxan and high-fat diets, serum total cholesterol remained at a high level (180 - 220 mg/dl) within 7 days of removing high-fat diets but reached normal level (120 - 140 mg/dl) after AC (0.40 g/kg per day) treatment. Also, AC (0.40 and 0.80 g/kg) significantly inhibited serum lipids from the increase in Triton WR-1339-induced hyperlipidemic mice. AC (0.01 - 100 microg/ml) selectively activated lipoprotein lipase (LPL) activity by 200% - 400% and significantly inhibited 3-hydroxyl-methyl glutaryl coenzyme A (HMGCoA) reductase activity by 20% - 49% in vitro. Furthermore, 2 months of fenofibrate (0.20 g/kg) administration particularly increased mice liver weights (0.0760 +/- 0.0110 g/g) while AC (0.40 g/kg) had no effect (0.0403 +/- 0.0047). Taken together, these results suggest that AC will be a new potential natural product for the treatment of hyperlipidemia that exerts its actions through mechanisms of inhibiting HMGCoA reductase and activating LPL activities. Its action mechanisms differentiate from those with fibrates but may be partly similar to those with statins. It is hopeful that AC may serve as the adjuvant for fibrates.

  12. Aplicación de las series temporales para predecir las propiedades de calidad de la piña (Ananas Comosus., variedad Cayena Lisa, durante el proceso de maduración a temperatura ambiente con respecto al tiempo real

    Directory of Open Access Journals (Sweden)

    Lazara Rangel Montes de Oca

    2013-01-01

    Full Text Available En el mundo actual el uso de las técnicas de análisis estadístico representa una novedosa alternativa en los procesos de poscose- cha, permitiendo ahorrar tiempo y gastos innecesarios a través del conocimiento del comportamiento de las propiedades que definen la calidad de los productos agrícolas durante su almacenamiento. La investigación a partir de trabajos realizados tiene como objetivo aplicar las series temporales para predecir las propiedades de calidad de la piña (Ananas Comosus., variedad Cayena Lisa, durante el proceso de maduración a temperatura ambiente con respecto al tiempo real. Para ello se realizó un análisis de los resultados obtenidos en trabajos que conciernen a esta temática los cuales poseen cierto reconocimiento, relacionando estos valores reales determinados con técnicas tradicionales con los modelos de predicción obtenidos (para masa, firmeza, SSC y pH utilizando el software especializado Statgraphics, versión 5.1. Como resultado principal se obtuvo que las series temporales que constituyen una herramienta capaz de predecir las propiedades de calidad de la piña en tiempo real durante el proceso de maduración a temperatura ambiente, mostrando que los mejores modelos de predicción para las propiedades de masa, el pH y la firmeza fueron los de Tendencia lineal. En el caso de la propiedad SSC, el modelo que mejor se ajustó a su comportamiento fue el de Suavizado exponencial lineal de Brown. Estos modelos de pronósticos constituyen un aporte significativo en su rama ya que permiten predecir valores de estas propiedades en la fruta de la piña a corto y mediano plazo con una confianza del 95%.

  13. Pharmacokinetic study of p-coumaric acid in mouse after oral administration of extract of Ananas comosus L. leaves.

    Science.gov (United States)

    Meng, Zhen; Wang, Wei; Xing, Dong-Ming; Lei, Fan; Lan, Jia-Qi; Du, Li-Jun

    2006-09-01

    Quantification of p-coumaric acid in mouse plasma following oral administration of Ananas comosus L. leaves was achieved by reversed-phase high-performance liquid chromatography using a mobile phase of water-acetonitrile (82:18, v/v) and UV detection at 310 nm. The method was linear (determination coefficient, r2 = 0.9997) within the tested range (0.04-1.28 microg/mL). Intra- and inter-day precision coefficients of variation and accuracy bias were acceptable (maximal CV value was 4.06% for intra-day and 4.19% for inter-day) over the entire range. The recoveries were 90.63, 97.98 and 100.01% for concentrations of 0.04, 0.32 and 1.28 microg/mL, respectively. This is a very rapid, sensitive and economical way to determine p-coumaric acid concentration in mouse plasma after oral administration of A. comosus leaves. The concentration-time curve was fitted to the one-compartment model. This is the first time that p-coumaric acid extracted from A. comosus leaves was detected by HPLC-UV method and its pharmacokinetic characteristic was comprehensively studied.

  14. Early histological, hormonal, and molecular changes during pineapple (Ananas comosus (L.) Merrill) artificial flowering induction.

    Science.gov (United States)

    Espinosa, Maita Eulalia Ávila; Moreira, Rafael Oliveira; Lima, André Almeida; Ságio, Solange Aparecida; Barreto, Horllys Gomes; Luiz, Sara Lazara Pérez; Abreu, Carlos Eduardo Aragón; Yanes-Paz, Ermis; Ruíz, Yanelis Capdesuñer; González-Olmedo, Justo Lorenzo; Chalfun-Júnior, Antonio

    2017-02-01

    Natural flowering can cause serious scheduling problems in the pineapple (Ananas comosus) industry and increase harvest costs. Pineapple flowering is thought to be triggered by increased ethylene levels and artificial forcing of pineapple flowering is a common practice to promote flowering synchronisation. However, little is known about the early hormonal and molecular changes of pineapple flowering induction and development. Here, we aimed to analyse the molecular, hormonal, and histological changes during artificial pineapple flowering by Ethrel(®)48 treatment. Histological analyses of the shoot apical meristem, leaf gibberellic acid (GA3), and ethylene quantification were carried out during the first 72h after Ethrel(®)48 treatment. Expression profiles from ethylene biosynthesis (AcACS2 and AcACO1), gibberellin metabolism (AcGA2-ox1 and AcDELLA1), and flower development (FT-like gene (AcFT), LFY-like gene (AcLFY), and a PISTILLATA-like gene (AcPI)) genes were analysed during the first 24h after Ethrel(®)48 treatment. Differentiation processes of the shoot apical meristem into flower buds were already present in the first 72h after Ethrel(®)48 treatment. Ethrel(®)48 lead to a reduction in GA3 levels, probably triggered by elevated ethylene levels and the positive regulation AcGA2-ox1. AcLFY activation upon Ethrel(®)48 may also have contributed to the reduction of GA3 levels and, along with the up-regulation of AcPI, are probably associated with the flower induction activation. AcFT and AcDELLA1 do not seem to be regulated by GA3 and ethylene. Decreased GA3 and increased ethylene levels suggest an accumulation of AcDELLA1, which may display an important role in pineapple flowering induction. Thus, this study shows that molecular, hormonal, and histological changes are present right after Ethrel(®)48 treatment, providing new insights into how pineapple flowering occurs under natural conditions.

  15. Leaf malate and succinate accumulation are out of phase throughout the development of the CAM plant Ananas comosus.

    Science.gov (United States)

    Rainha, N; Medeiros, V P; Ferreira, C; Raposo, A; Leite, J P; Cruz, C; Pacheco, C A; Ponte, D; Silva, A B

    2016-03-01

    In plants with Crassulacean Acid Metabolism (CAM), organic acids, mainly malate are crucial intermediates for carbon fixation. In this research we studied the circadian oscillations of three organic anions (malate, citrate, and succinate) in Ananas comosus, assessing the effect of season and plant development stage. Seasonal and plant development dependencies were observed. The circadian oscillations of malate and citrate were typical of CAM pathways reported in the literature. Citrate content was quite stable (25-30 μmol g(-1) FW) along the day, with a seasonal effect. Succinate was shown to have both diurnal and seasonal oscillations and also a correlation with malate, since it accumulated during the afternoon when malate content was normally at a minimum, suggesting a possible mechanistic effect between both anions in CAM and/or respiratory metabolisms.

  16. A novel aspartic acid protease gene from pineapple fruit (Ananas comosus): cloning, characterization and relation to postharvest chilling stress resistance.

    Science.gov (United States)

    Raimbault, Astrid-Kim; Zuily-Fodil, Yasmine; Soler, Alain; Cruz de Carvalho, Maria H

    2013-11-15

    A full-length cDNA encoding a putative aspartic acid protease (AcAP1) was isolated for the first time from the flesh of pineapple (Ananas comosus) fruit. The deduced sequence of AcAP1 showed all the common features of a typical plant aspartic protease phytepsin precursor. Analysis of AcAP1 gene expression under postharvest chilling treatment in two pineapple varieties differing in their resistance to blackheart development revealed opposite trends. The resistant variety showed an up-regulation of AcAP1 precursor gene expression whereas the susceptible showed a down-regulation in response to postharvest chilling treatment. The same trend was observed regarding specific AP enzyme activity in both varieties. Taken together our results support the involvement of AcAP1 in postharvest chilling stress resistance in pineapple fruits.

  17. Leaf margin phenotype-specific restriction-site-associated DNA-derived markers for pineapple (Ananas comosus L.).

    Science.gov (United States)

    Urasaki, Naoya; Goeku, Satoko; Kaneshima, Risa; Takamine, Tomonori; Tarora, Kazuhiko; Takeuchi, Makoto; Moromizato, Chie; Yonamine, Kaname; Hosaka, Fumiko; Terakami, Shingo; Matsumura, Hideo; Yamamoto, Toshiya; Shoda, Moriyuki

    2015-06-01

    To explore genome-wide DNA polymorphisms and identify DNA markers for leaf margin phenotypes, a restriction-site-associated DNA sequencing analysis was employed to analyze three bulked DNAs of F1 progeny from a cross between a 'piping-leaf-type' cultivar, 'Yugafu', and a 'spiny-tip-leaf-type' variety, 'Yonekura'. The parents were both Ananas comosus var. comosus. From the analysis, piping-leaf and spiny-tip-leaf gene-specific restriction-site-associated DNA sequencing tags were obtained and designated as PLSTs and STLSTs, respectively. The five PLSTs and two STSLTs were successfully converted to cleaved amplified polymorphic sequence (CAPS) or simple sequence repeat (SSR) markers using the sequence differences between alleles. Based on the genotyping of the F1 with two SSR and three CAPS markers, the five PLST markers were mapped in the vicinity of the P locus, with the closest marker, PLST1_SSR, being located 1.5 cM from the P locus. The two CAPS markers from STLST1 and STLST3 perfectly assessed the 'spiny-leaf type' as homozygotes of the recessive s allele of the S gene. The recombination value between the S locus and STLST loci was 2.4, and STLSTs were located 2.2 cM from the S locus. SSR and CAPS markers are applicable to marker-assisted selection of leaf margin phenotypes in pineapple breeding.

  18. Determinación de las propiedades de calidad de la piña (Ananas Comosus variedad Cayena Lisa almacenada a temperatura ambiente

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    Yelene García Tain

    2011-01-01

    Full Text Available La calidad es un atributo complejo de necesario conocimiento para la cosecha y poscosecha de las frutas en sus diferentes formas de consumo. El presente trabajo tiene como objetivo determinar las propiedades de calidad de la piña (Ananas Comosus, variedad Cayena Lisa, almacenadas a temperatura ambiente. Durante el análisis, 50 frutas fueron almacenadas durante un período de 9 días, evaluando 5 frutas diariamente, fueron determinados: pérdidas de peso, IC*, firmeza, SSC, pH, firmeza al tacto, aroma y apariencia general, relacionando cada propiedad antes mencionada con respecto al tiempo de almacenamiento a través de la herramienta de cálculo Excel, Microsoft Office y un panel de expertos monitoreo las propiedades organolépticas de las frutas. Como principales resultados fueron establecidos los estados de maduración representados en una carta de color, además el período óptimo para el consumo se propone a partir del 5to al 7mo días de almacenadas, mientras a partir del 8vo día está apta para ser procesada industrialmente. Todas las propiedades estudiadas demostraron alta dependencia con respecto al tiempo de almacenamiento.

  19. Poly(N-Isopropylacrylamide)-co-Acrylamide Hydrogels for the Controlled Release of Bromelain from Agroindustrial Residues of Ananas comosus.

    Science.gov (United States)

    Croisfelt, Fernanda; Martins, Bianca C; Rescolino, Robson; Coelho, Diego F; Zanchetta, Beatriz; Mazzola, Priscila G; Goulart, Luis Ricardo; Pessoa, Adalberto; Tambourgi, Elias B; Silveira, Edgar

    2015-12-01

    This works reports the purification of bromelain extracted from Ananas comosus industrial residues by ethanol purification, its partial characterization from the crude extract as well as the ethanol purified enzyme, and its application onto poly(N-isopropylacrylamide)-co-acrylamide hydrogels. Bromelain was recovered within the 30-70 % ethanol fraction, which achieved a purification factor of 3.12-fold, and yielded more than 90 % of its initial activity. The resulting purified bromelain contained more than 360 U · mg(-1), with a maximum working temperature of 60 °C and pH of 8.0. Poly(N-isopropylacrylamide)-co-acrylamide hydrogels presented a swelling rate of 125 %, which was capable of loading 56 % of bromelain from the solution, and was able to release up to 91 % of the retained bromelain. Ethanol precipitation is suitable for bromelain recovery and application onto poly(N-isopropylacrylamide)-co-acrylamide hydrogels based on its processing time and the applied ethanol prices.

  20. Effect of ethanolic extracts of Ananas comosus L. leaves on insulin sensitivity in rats and HepG2.

    Science.gov (United States)

    Xie, Weidong; Wang, Wei; Su, Hui; Xing, Dongming; Pan, Yang; Du, Lijun

    2006-08-01

    Ethanolic extracts of Ananas comosus L. leaves (AC) enriched with phenols have hypoglycemic activity in diabetic rats. Here, we investigated the effect of AC on insulin sensitivity in rats and HepG2. In high-fat diet-fed and low-dose streptozotozin-treated diabetic Wistar rats subjected to challenge with exogenous human insulin, AC treatment at an oral dose of 0.40 g/kg could significantly improve sensitivity to exogenous insulin. After a sub-acute treatment, AC also could inhibit the development of insulin resistance in high-fat diet-fed and low-dose streptozotozin-treated diabetic rats following the test of loss of tolbutamide-induced blood glucose lowering action. For intravenous insulin/glucose infusion test, high-fat diet-fed and low-dose alloxan-treated Wistar rats were associated with insulin resistance, which was improved after AC or fenofibrate treatment. AC application inhibited the development of insulin resistance in HepG2 cells. The above animal models were well developed to simulate type 2 diabetes. Taken together, our results suggest that AC may improve insulin sensitivity in type 2 diabetes and could be developed into a new potential natural product for handling of insulin resistance in diabetic patients.

  1. FORMULATION DEVELOPMENT OF TOPICAL CREAM LOADED WITH ANANAS COMOSUS EXTRACT: IN VIVO EVALUATION FOR CHANGES IN SKIN BARRIER FUNCTION USING BIOPHYSICAL TECHNIQUES.

    Science.gov (United States)

    Arshad, Atif I; Khan, Shoaib H M; Akhtar, Naveed

    2016-01-01

    The prime objective of current investigation was to develop a topical skin care cream (w/o) loaded with Ananas comosus extract versus placebo control, and evaluated non-invasively for changes in skin barrier function i.e., epidermal hydration levels and transepidermal water loss (TEWL), on healthy human volunteers. Active cream carrying 2% extract of Ananas comosus in the internal phase of w/o emulsion was prepared while placebo contained no extract. Stability assessment of both creams was performed at various storage conditions 8, 25, 40 degrees C, 40 degrees C + 75% RH (relative humidity) and 50 degrees C. Effects on epidermal hydration and TEWL were observed by applying active cream at one side and placebo on the other side of face by 11 healthy human volunteers during 12 weeks period using Corneometer MPA5 and Tewameter MPA5. Results indicated that both creams (active and placebo) remained stable at all storage conditions. All samples manifested non-Newtonian, shear thinning behavior with increasing shear rate, whereas statistical interpretation indicated that effects of active cream were superior than placebo, as it significantly (p = 0.05) improves the epidermal hydration levels up to 56.74% and reduces TEWL up to -73.19% at the end of study period compared to baseline value. The surface evaluation of living skin (SELS) parameters SEr, SEsc, SEsm, SEw were also assessed and indicated a significant (p = 0.05) reduction. Conclusively, creams loaded with Ananas comosus extract exhibit better physicochemical stability and represent a propitious improvement in skin barrier function, used as a functional moisturizing and anti-aging ingredient in topical skincare products.

  2. Meta-análisis de los subproductos de piña (Ananas comosus) para la alimentación animal.

    OpenAIRE

    Michael López-Herrera; Rodolfo WingChing-Jones; Augusto Rojas-Baurillón

    2014-01-01

    Meta-análisis de los subproductos de piña (Ananas comosus) para la alimentación animal. El objetivo del presente trabajo fue determinar el uso potencial de los sub-productos del cultivo de la piña para su empleo en la dieta de animales rumiantes. Se realizó una recopilación y análisis estadístico de la información obtenida de diferentes investigaciones realizadas hasta el año 2013 a nivel mundial, para la caracterización nutricional de la piña: planta entera, rastrojo (hojas, hijos y t...

  3. Characterization of active phenolic components in the ethanolic extract of Ananas comosus L. leaves using high-performance liquid chromatography with diode array detection and tandem mass spectrometry.

    Science.gov (United States)

    Ma, Chao; Xiao, Sheng-yuan; Li, Zhen-guo; Wang, Wei; Du, Li-jun

    2007-09-21

    HPLC-DAD-MS was utilized to investigate the phytochemical constituents in ethanolic extract of Ananas comosus L. leaves (EEACL) responsible for antidiabetic, antihyperlipidemic and antioxidative effects. Eight phenylpropane diglycerides, together with two hydroxycinnamic acids, three hydroxycinnamoyl quinic acids, four phenylpropane monoglycerides, three flavones and six phenylpropanoid glycosides were detected, and their proposed structures were elucidated based on HPLC retention time, UV and MS profiles. Meanwhile, a new HPLC-DAD-MS method was established for the identification and characterization of phenylpropane diglycerides in natural plants.

  4. Genomic study of the absorption mechanism of p-coumaric acid and caffeic acid of extract of Ananas comosus L. leaves.

    Science.gov (United States)

    Dang, Yun-jie; Zhu, Chun-yan

    2015-03-01

    Cardiac disease has emerged as the leading cause of death worldwide, and food rich in phenolic acids has drawn much attention as sources of active substances of hypolipidemic drug. Ananas comosus L. (pineapple) is one of the most popular tropical and subtropical fruits. Isolated from pineapple leaves, EAL(Extract of Ananas Comosus L. Leaves) is rich in phenolic acids, such as p-coumaric acid, caffeic acid, and other phenolics, highly relevant to the putative cardiovascular-protective effects, which suggests its potential to be a new plant medicine for treatment of cardiac disease, but little is known about absorption, distribution, metabolism, and excretion of EAL in animals or human beings. In this study, we employed cDNA microarray, Caco-2 cell lines, and rat intestinal model to explore the absorption behavior of p-coumaric acid and caffeic acid in EAL. The permeation of 2 substances was concentration and time dependent. Results also indicated that monocarboxylic acid transporter was involved in the transepithelial transport of p-coumaric acid and caffeic acid.

  5. Respiratory properties and malate metabolism in Percoll-purified mitochondria isolated from pineapple, Ananas comosus (L.) Merr. cv. smooth cayenne.

    Science.gov (United States)

    Hong, Hoang Thi Kim; Nose, Akihiro; Agarie, Sakae

    2004-10-01

    An investigation was made of the respiratory properties and the role of the mitochondria isolated from one phosphoenolpyruvate carboxykinase (PCK)-CAM plant Ananas comosus (pineapple) in malate metabolism during CAM phase III. Pineapple mitochondria showed very high malate dehydrogenase (MDH), and low malic enzyme (ME) and glutamate-oxaloacetate transaminase (GOT) activities. The mitochondria readily oxidized succinate and NADH with high rates and coupling, while they only oxidized NADPH in the presence of Ca(2+). Pineapple mitochondria oxidized malate with low rates under most assay conditions, despite increasing malate concentrations, optimizing pH, providing cofactors such as coenzyme A, thiamine pyrophosphate, and NAD(+), and supplying individually external glutamate or GOT. However, providing glutamate and GOT simultaneously strongly increased the rates of malate oxidation. The OAA easily permeated the mitochondrial membranes to import into or export out of pineapple mitochondria during malate oxidation, but the mitochondria did not consume external Asp or alpha-KG. These results suggest that OAA played a significant role in the mitochondrial malate metabolism of pineapple, in which malate was mainly oxidized by active mMDH to produce OAA which could be exported outside the mitochondria via a malate-OAA shuttle. Cytosolic GOT then consumed OAA by transamination in the presence of glutamate, leading to a large increase in respiration rates. The malate-OAA shuttle might operate as a supporting system for decarboxylation in phase III of PCK-CAM pineapple. This shuttle system may be important in pineapple to provide a source of energy and substrate OAA for cytosolic PCK activity during the day when cytosolic OAA and ATP was limited for the overall decarboxylation process.

  6. Characterization of the third SERK gene in pineapple (Ananas comosus) and analysis of its expression and autophosphorylation activity in vitro.

    Science.gov (United States)

    Ma, Jun; He, Yehua; Hu, Zhongyi; Xu, Wentian; Xia, Jingxian; Guo, Cuihong; Lin, Shunquan; Chen, Chengjie; Wu, Chenghou; Zhang, Junli

    2014-09-01

    Two somatic embryogenesis receptor-like kinase genes (identified as AcSERK1 and AcSERK2) have previously been characterized from pineapple (Ananas comosus). In this work, we describe the characterization of a third gene (AcSERK3) in this family. AcSERK3 had all the characteristic domains and shared extensive sequence homology with other plant SERKs. AcSERK3 expression was studied by in situ hybridization and quantitative real-time PCR to analyze its function. Intense in situ hybridization signals were observed only in single competent cells and competent cell clusters; no hybridization signal was detected in the subsequent stages of somatic embryogenesis. AcSERK3 was highly expressed in embryogenic callus compared to other organs, e.g., 20-80 fold more than in anther but similar to that of non-embryogenic callus, which was 20-50 fold that of anther. AcSERK3 expression in root was 80 fold higher than in anther and the highest amongst all organs tested. These results indicate that AcSERK3 plays an important role in callus proliferation and root development. His-tagged AcSERK3 protein was successfully expressed and the luminescence of His6-AcSERK3 protein was only ∼5% of that of inactivated AcSERK3 protein and reaction buffer without protein, and 11.3% of that of an extract of host Escherichia coli pET-30a. This finding confirmed that the AcSERK3 fusion protein had autophosphorylation activity.

  7. Molecular characterization and pathogenicity of Erwinia spp. associated with pineapple [Ananas comosus (L. Merr.] and papaya (Carica papaya L.

    Directory of Open Access Journals (Sweden)

    Ramachandran Kogeethavani

    2015-12-01

    Full Text Available The Erwinia species are well-known pathogens of economic importance in Malaysia causing serious damage to high-value fruit crops that include pineapple [Ananas comosus (L. Merr.] and papaya (Carica papaya L..The 16S rRNA sequence using eubacteria fD1 and rP2 primers, identified two bacteria species; Dickeya zeae from pineapple heart rot, and Erwinia mallotivora from papaya dieback. Phylogenetic analysis based on the neighbor-joining method indicated that all the bacterial isolates clustered in their own taxa and formed monophyletic clades. From the pathogenicity test, all isolates of D. zeae and E. mallotivora showed pathogenic reactions on their respective host plants. Genetic variability of these isolates was assessed using repetitive sequence-based PCR (rep-PCR fingerprinting. The results indicated interspecies, and intraspecies variation in both species’ isolates. There were more polymorphic bands shown by rep-PCR fingerprints than enterobacterial repetitive intergenic consensus (ERIC and BOX- PCRs, however both species’ isolates produced distinguishable banding patterns. Unweighted pair-group method with arithmetic averages (UPGMA cluster analysis indicated that all Dickeya and Erwinia isolates from the same species were grouped in the same main cluster. Similarity among the isolates ranged from 77 to 99%. Sequencing of 16S rRNA using eubacteria fD1 and rP2 primers, and rep-PCR fingerprinting revealed diversity among Dickeya and Erwinia isolates. But this method appears to be reliable for discriminating isolates from pineapple heart rot and papaya dieback.

  8. META-ANÁLISIS DE LOS SUBPRODUCTOS DE PIÑA (Ananas comosus PARA LA ALIMENTACIÓN ANIMAL

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    Michael L\\u00F3pez-Herrera

    2014-01-01

    Full Text Available Meta-análisis de los subproductos de piña (Ananas comosus para la alimentación animal. El objetivo del presente trabajo fue determinar el uso potencial de los sub-productos del cultivo de la piña para su empleo en la dieta de animales rumiantes. Se realizó una recopilación y análisis estadístico de la información obtenida de diferentes investigaciones realizadas hasta el año 2013 a nivel mundial, para la caracterización nutricional de la piña: planta entera, rastrojo (hojas, hijos y tallo, corona, corazón, cáscara y pulpa de la fruta, tallo y raíces, para optimizar su uso en la alimentación de animales rumiantes. Se comparó el efecto que tiene el manejo que se proporciona a los subproductos, siendo las variables de esta comparación, el proceso de ensilaje, el secado o el material fresco sin ningún tipo de aditivo. La composición nutricional de los materiales varió de acuerdo a la parte de la planta. Las raíces presentaron el mayor contenido de materia seca, fibra detergente neutro, fibra detergente ácido, lignina y cenizas. Mientras que las coronas y los rastrojos mostraron mayores contenidos de proteína cruda y energía, pero menor contenido de materia seca. Hubo una diferencia entre el proceso de deshidratado y el material fresco o ensilado, no así entre los materiales con alto contenido de humedad. Los subproductos obtenidos de los sistemas de producción de piña, tuvieron contenidos de energía y nutrimentos que permitirían su utilización como parte de la ración en la alimentación de rumiantes sin perjudicar su desempeño productivo.

  9. COMPARISON OF EDTA AND EDDS ENHANCTED PHYTOEXTRACTION OF Cr AND Pb FROM CONTAMINATED SOIL BY ANANAS COMOSUS (L. MERR.

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    Pantawat Sampanpanish

    2014-01-01

    Full Text Available The effects of chelating agents on Chromium (Cr and lead (Pb absorption were studied by planting pineapple, Ananas comosus (L. Merr.in contaminated soil. All plant samples were grown in a nursery for 30 days and then separated into seven sets: Set (1 had nothing added (Blank; (2 had Pb added as Pb(II Nitrate (Pb(NO32 at 500 mg kg-1 soil; (3 added Pb(II nitrate and EDTA, a chelating agent; (4 contained both Pb(II nitrate and EDDS, a second chelating agent; (5 only added Cr as potassium dichromate (K2Cr2O7, at 400 mg kg-1 soil; (6 was treated with Cr (potassium dichromate plus EDTA; and (7 contained both Cr (potassium dichromate and EDDS. The chelating agent concentrations were 2 millimoles per kilogram soil. Soil and plant contamination levels were measured by analyzing the Cr, Cr(VI and total Pb after growing for 30, 60, 90 and 120 days. The analysis divided the plant samples into two parts: Aboveground and underground. Plant growth was also analyzed by dry weight, root length and expression of toxicity through withered leaves and yellow leaf symptoms. The results of this study indicate that after 60 days, the EDTA agent had the highest Pb absorption efficiency, with the plant sample absorbing 288.14 mg Pb per kg soil in the aboveground part and 796.66 mg kg-1 soil in the underground part. The EDTA agent had high Cr absorption efficiency, with the plant sample absorbing Cr at 545.72 mg kg-1 soil in the aboveground part and 2267.99 mg kg-1 soil in the underground part after 90 days. The EDTA and EDDS agents did not affect pineapple growth and expression of toxicity symptoms were statistically significant (p≤0.05 compared with the control sets.

  10. Morpho-physiological changes in pineapple plantlets [Ananas comosus (L. merr.] during acclimatization Mudanças morfofisiológicas em plântulas de abacaxi [Ananas comosus (L. Merr.] durante a aclimatização

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    Ariel Villalobo

    2012-12-01

    Full Text Available Pineapple producing countries lack good quality propagation material to expand cultivars into new areas. Previously, research protocols may increase the offer of high quality plantlets and speed the introduction of new pineapple cultivars. The present work is to evaluate the morpho-physiological changes in plantlets of pineapple [Ananas comosus (L. Merr. 'MD-2'] during the acclimatization phase. Plantlets were acclimatized under 80% relative humidity, 25.5 °C temperature and photosynthetic photon flux of 400-500 µmol m-2 s-1 as average for 45 d under natural photoperiods. All measurements (plant length, number of leaves and roots, fresh weight, width and length of leaf 'D', net photosynthesis and total transpiration rate were carried out at the end of in vitro rooting phase coincident with 0 d of acclimatization and at 15, 30 and 45 d thereafter. Photosynthetic activity of in vitro plantlets did not increase during the first 30 d of the acclimatization phase. After 30 d, photosynthetic activity ranged from 5.72 to 9.36 µmol CO2 m-2 s-1 while total transpiration ranged from 6.0 to 1.42 mmol H2O m-2 s-1. During the first 30 days there were no significant differences in number of leaves, length or width of the longest ('D' leaf (cm or plant length (cm. However, after 45 days plant fresh weight (g, length and width of the 'D' leaf (cm and root number all increased significantly, while transpiration (mmol H2O m-2 s-1 declined. There were small but significant decreases in chlorophyll a and b (µg g-1 mf.. Increased photosynthetic activity after 30 d shows that the increase in light intensity and the reduction of relative humidity during acclimatization did not constitute inhibitory factors.Os países produtores do abacaxi precisam de mudas de boa qualidade para a introdução de novas culturas nas áreas de cultivo. Os protocolos de pesquisa podem aumentar a oferta de mudas de alta qualidade e acelerar a introdução de novas culturas do abacaxi

  11. Chemical and antioxidant properties of snake tomato (Trichosanthes cucumerina) juice and Pineapple (Ananas comosus) juice blends and their changes during storage.

    Science.gov (United States)

    Bamidele, Oluwaseun P; Fasogbon, Mofoluwaso B

    2017-04-01

    Juice blends made from the mixture of snake tomato (Trichosanthes cucumerina) and Pineapple (Ananas comosus) fruits were analyzed for pH, antioxidant properties, total titratable acidity, vitamin C, lycopene and total phenolic contents after different blend ratios were made. The addition of snake tomato juice increased the vitamin C, total carotene, lycopene and antioxidant properties of the juice blends. The radical scavenging properties of juice blends containing a higher ratio of snake tomato were higher and samples stored at room temperature (29°C) showed an increase in antioxidant properties compared to samples stored at 4°C. In conclusion, snake tomato juice up to 50% may be added to Pineapple juice to make a healthy juice blend.

  12. Tissue Culture and Rapid Propagation of Edible Ananas comosus%食用菠萝的组织培养和快速繁殖

    Institute of Scientific and Technical Information of China (English)

    陈健妙; 甘铨; 刘联

    2001-01-01

    @@ 1 植物名称菠萝(Ananas comosus). 2 材料类别吸芽. 3 培养条件以MS为基本培养基.(1)诱导芽分化培养基:1/2MS+NAA 1 mg@L-1(单位下同)+0.2%活性炭;(2)增殖培养基:MS+NAA0.2+6-BA 2;(3)生根培养基:1/2MS+IBA 2.5+0.5%活性炭.以上培养基均用0.5%卡拉胶,3%蔗糖,培养基pH值5.8,培养温度为(25±2)℃,光照度2 000 lx,光照时间10 h@d-1.

  13. The effects of Ananas comosus L. leaves on diabetic-dyslipidemic rats induced by alloxan and a high-fat/high-cholesterol diet.

    Science.gov (United States)

    Xie, Weidong; Xing, Dongming; Sun, Hong; Wang, Wei; Ding, Yi; Du, Lijun

    2005-01-01

    The aim of this study is to demonstrate the effects of Ananas comosus L. leaves on diabetic-dyslipidemic rats. Hypoglycemic and hypolipidemic activities of the ethanolic extract of Ananas comosus L. leaves (EEACL) were evaluated in normal and alloxan-induced diabetic rats by oral glucose tolerance test and an olive oil load test. Anti-diabetic, anti-hyperlipidemic and anti-oxidative activities of EEACL were also investigated in diabetic-dyslipidemic rats induced by alloxan and a high-fat/high-cholesterol diet. EEACL at the dose of 0.40 g/kg significantly inhibited the increase in blood glucose in diabetic rats in oral glucose tolerance test, but did not cause any hypoglycerimic activity in normal rats. It also significantly inhibited the increase in postprandial triglycerides (TG) levels in both normal and diabetic rats in olive oil load test. After 15 days of treatment of diabetic dyslipidemic rats, EEACL significantly decreased blood glucose (-51.0%, P < 0.01), TG (-50.1%, P < 0.01), TC (-23.3%, P < 0.01), LDL-c (-47.9%, P < 0.01) and glycated albumin (-25.4%, P < 0.01) levels, significantly increased serum high-density lipoprotein cholesterol levels (66.2%, P < 0.01) and prevented lower body weight of diabetes (11.8%, P < 0.05), significantly lowered lipid peroxidation productions of blood (-27.8%, P < 0.01), brain (-31.6%, P < 0.05), liver (-44.5%, P < 0.01) and kidneys (-72.2%, P < 0.05) compared with those in untreated diabetic dyslipidemic rats. These data suggest that EEACL has anti-diabetic, anti-dyslipidemic and anti-oxidative activities, which may be developed into a new plant medicine for treatment of diabetes and its complications.

  14. Cardioprotective potential of hydro-alcoholic fruit extract ofAnanas comosus against isoproterenol induced myocardial infraction in Wistar Albino rats

    Institute of Scientific and Technical Information of China (English)

    Priya Saxena; Dharamveer Panjwani

    2014-01-01

    Objectives:To evaluate the cardioprotective effects of hydro alcoholic extract ofAnanas comosus (A. comosus)(HEAC), onIsoproterenol(ISO) induced myocardial infarction inAlbinoWistar rats. Methods:Myocardial infarction was induced byIsoproterenol(85 mg/kg,s.c.) for two consecutive days at an interval of24 h.Rats were pretreated withHEAC(200-400 mg/kg/day, oral) for a period of30 days andIsoproterenol(ISO) was injected on31st and32nd day and after24 h blood was collected through retro-orbital plexus for the estimation of biochemical parameters and histopathological studies were also performed.Results:In the present study,ISO administration significantly elevated the cholesterol, low density lipoprotein, very low density lipoprotein, triglycerides, alanine aminotransferase and aspartate aminotransferase levels while it decreases high density lipoprotein and total protein in plasma and administration ofHEAC decreases the level of cholesterol, low density lipoprotein, very low density lipoprotein, triglycerides, alanine aminotransferase and aspartate aminotransferase levels while it increases high density lipoprotein and total protein levels.Pretreatment with theHEAC protected the cardiotoxicity induced by Isoproterenol.The histopathological findings of theISO-induced myocardium showed infracted zone with inflammatory cells, lipid droplets, myocardial necrosis and vacuolization of myofibrils which were reduced by the pretreatment ofHEAC.Conclusion:It can be concluded thatHEAC possess cardioprotective activity againstIsoproterenol induced myocardial infarction in rats.

  15. Observaciones sobre características, distribución y daños de sinfilidos (Symphyla y otros organismos del suelo en cultivos de piña, Ananas comosus, del Valle

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    Zuluaga C. José Iván

    1988-12-01

    Full Text Available Como en los últimos años los cultivos de piña, Ananas comosus, del Valle, han sido atacados por sinfílifos, clasificados por el Dr. Evert Lindquist como Scutigerella inmaculata, se estudiaron aspectos biológicos, distribución, daños y relaciones con algunos microorganismos. Mediante encuestas e inspecciones al suelo y plantas se verificó su presencia en Palmira, Pradera, Buga, Buga la grande, Sevilla, Dagua y Darién, principales zonas productoras de piña en este departamento. Ensayos en laboratorio demostraron Que S. inmaculata es de hábitos alimenticios saprófagos y/o fitófagos y de esta forma puede producir daños a las raíces de la pila, causando el síntoma de "escoba", enrojecimiento al follaje y debilidad en la planta; también se demostró que 3 sinfílidos/plante producen los síntomas del ataque. El sinfílido se presenta desde semillero, siendo los ataques tempranos los más graves, causando pérdidas en raíces del 66% aproximadamente.Pineapples culture Ananas comosus of Valle, they have been attacked by symphylids last vean (Symphyla, classified by Dr. Evert Lindquist like Scutigerella inmaculata. Biologie aspects, distribution, harms and relationships with some microorganisms some studied in this work. This has a wide distribution on Valle and through surveys and inspections over soil and plants their presance was verified in Palmira, Pradera, Buga; Bugalagrande, Sevilla, Dagua y Darién, the main producer pineapple zones in this department. Laboratory essays showed that this symphilids has saphrophytes and plant eater food habits and by this way could produce harm the pineapples roots causing symptom "broom", foliage getting red color and weakness in plant; also is shown that three symphylids per plant produce tipical symptom of attacking. It is made evident from seed bed being early attacks were more grave causing' root losses about 66% (percent aproximatilly.

  16. DIVERGÊNCIA GENÉTICA ENTRE DOZE GENÓTIPOS DE ABACAXIZEIRO (Ananas comosus L, Merril. ESTIMADA POR ANÁLISE DE MARCADORES RAPD GENETIC DIVERGENCE AMONG TWELVE GENOTYPES OF PINEAPLE (Ananas comosus L, Merril. ESTIMATED BY RAPD MARKERS

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    CÍCERO DONIZETE PEREIRA

    2001-08-01

    Full Text Available Por meio de estudos moleculares, este trabalho determinou a distância genética entre 12 genótipos de A. comosus por marcadores RAPD (Random Amplified Polymorphic DNA, utilizando 11 "primers" decâmeros da OPERON Technologies Inc. Dos 12 genótipos , 1 foi proveniente da Jamaica, 2 do Estado do Acre (Quinari e RBR-1, 2 do Estado do Maranhão (Turiaçu e São Domingos, 3 do Estado do Piauí (Cefas, Floriano-1 e Floriano-2, 2 do Estado da Bahia (Monte Alegre-1 e Monte Alegre-2 e 2 de Minas Gerais (Pérola e Smouth Cayenne. Pela análise de "cluster", utilizando o método de UPGMA, foi constatada uma grande divergência entre os genótipos de A. comosus estudados com a separação destes em dois grupos a uma distância genética de 31,1%.Through molecular studies, the genetic distance among 12 genotypes of A. comosus for RAPD markers (Random Amplified Polymorphic DNA was determined using 11 primers (decamers of the OPERON Technologies Inc. From the 12 genotypes, 1 came from Jamaica, 2 from the State of Acre (Quinari and RBR-1, 2 from the State of Maranhão (Turiaçu and São Domigos, 3 the State of Piauí (Cefas, Floriano-1 and Floriano-2, 2 from the State of Bahia (Monte Alegre-1 and Monte Alegre-2 and 2 from Minas Gerais (Pérola and Smooth Cayenne. By the analysis of cluster, using the method of UPGMA, a great divergence was verified among the genotypes of A. comosus studied or tima separation in two groups of a genetic distance of 31,1%.

  17. Análisis comparativo de la cinética de deshidratación Osmótica y por Flujo de Aire Caliente de la Piña (Ananas Comosus, variedad Cayena lisa

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    Annia García Pereira

    2013-01-01

    Full Text Available Los procesos tecnológicos de deshidratación han sido algunos de los métodos más utilizados para la conservación de frutas y vegetales. El presente trabajo tiene como objetivo analizar comparativamente la cinética de la deshidratación Osmótica (DO y por Flujo de Aire Caliente (DAC de la Piña (Ananas Comosus, variedad Cayena lisa para la Agroindustria. Durante el análisis se determinaron las propiedades físico-mecánicas de las frutas frescas y de los cubos deshidratadas (masa, firmeza, talla, pH, y contenido de sólidos solubles (SSC, posteriormente las frutas fueron troceadas en cubos de 1 x 5 ±0.02 cm para ser sometidas a tratamientos de Deshidratación Osmótica (DO (60 °C durante 10 h y por Deshidratación por Flujo de Aire Caliente (DAC (60°C durante 5 h. Se monitoreó además la variación de la masa, la firmeza y el porcentaje de pérdida de peso durante ambos procesos. Para el procesamiento estadístico de los datos se utiliza el software especializado Statgraphics Plus en su versión 5.1. Como resultado se obtuvo que la variabilidad alcanzada a partir de la diferencia entre los valores iniciales y finales de la masa, firmeza y el aumento del porcentaje de pérdida de peso fue inferior para la fruta sometida al proceso tecnológico de DO con valores de 49,4; 86 y 64,17%, que para la obtenida por DAC con valores 80; 88,3; y 80%, respectivamente, resultando también el método que mayor variabilidad aportó entre las propiedades estudiadas.

  18. Valor nutritivo do resíduo da indústria processadora de abacaxi (Ananas comosus L. em dietas para ovinos. 1. Consumo, digestibilidade aparente e balanços energético e nitrogenado Nutritive value of pineapple by-product (Ananas comosus L. in diets for sheep. 1. Intake, apparent digestibility, energetic and nitrogenous balance

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    M.C.P. Rogério

    2007-06-01

    Full Text Available O presente estudo foi conduzido para avaliar a influência da inclusão do resíduo do processamento de abacaxi (Ananas comosus L. nas dietas sobre os consumos (por unidade de tamanho metabólico - UTM e coeficientes de digestibilidade da matéria seca (MS, matéria orgânica (MO, proteína bruta (PB, extrato etéreo (EE, fibra detergente neutro (FDN, fibra detergente ácido (FDA, hemiceluloses (HCEL, celulose (CEL e energia de dietas experimentais isofibrosas e isoprotéicas. Avaliaram-se também os balanços nitrogenados e de energia dos animais. Vinte ovinos machos, inteiros receberam rações com quatro níveis de inclusão do resíduo (0; 11%; 16%; 27% em base de matéria seca, em delineamento inteiramente ao acaso, com cinco repetições por tratamento. Maiores consumos de matéria seca foram evidenciados quando as rações continham 11% do resíduo. Maiores consumos de proteína bruta, fibra detergente neutro, fibra detergente ácido e celulose (g/UTM e energia metabolizável (kcal/UTM foram apresentados por animais alimentados com dietas contendo 11% de resíduo em relação ao das dietas com 27% de inclusão (PThe influence of increasing levels of pineapple by-product (Ananas comosus L. on intake (unit metabolic size - UMS and apparent digestibility (% of dry matter (DM, organic matter (OM, crude protein (CP, ether extract (EE, neutral detergent fiber (NDF, acid detergent fiber (ADF, hemicelluloses (HCEL and cellulose (CEL and energy of isofibrous and isoproteic experimental diets of sheep were evaluated. Energetic and nitrogenous balance were also measured. Twenty rams were randomly allotted to four treatments in a completely randomized design with five replications. The treatments were formulated to contain zero; 11, 16 and 27% of pineapple by-product on DM basis. Higher dry matter intake was observed on 11% pineapple by-product diet. CP, NDF, ADF, CEL intake (g/UMS and metabolizable energy intake (kcal/UMS of 11% pineapple by

  19. Comparative study of kinetics of adsorption of methylene blue from aqueous solutions using cinnamon plant (Cinnamonum zeylanicum leaf powder and pineapple (Ananas comosus peel powder

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    Satish Dnyandeo Patil

    2012-07-01

    Full Text Available Batch adsorption of methylene blue (MB onto Cinnamon plant (Cinnamonum Zeylanicum leaf powder (CPLP and Pineapple (Ananas Comosus peel powder (PPP was investigated. Different parameters such as initial sorbate concentration, adsorbent dosage, pH, contact time, agitation speed, temperature and particle size. All isotherm models were found to be best fitting with high values of regression coefficient i.e. for Langmuir (R2 = 0.989 to 0.994 for CPLP and 0.993 to 0.995 for PPP, for Freundlich (R2 = 0.996 to 0.998 for CPLP and 0.995 for PPP and for Temkin (R2 = 0.983 to 0.995 for CPLP and 0.984 to 0.989 for PPP. The monolayer (maximum adsorption capacities (qm were found to be 250 and 333.333 mg/g for CPLP and PPP respectively. Lagergen pseudo -second order model best fits the kinetics of adsorption (R2 = 0.999 for CPLP and 1 for PPP. The amount of dye adsorbed at equilibrium qe(the obtained from Lagergen pseudo -second order model were found to be nearly same with the experimental data. Intra particle diffusion plot showed boundary layer effect and larger intercepts indicates greater contribution of surface sorption in rate determining step. Adsorption was found to be directly proportional to pH and temperature but inversely proportional to particle size. Thermodynamic analysis (∆G, ∆H and ∆S values showed adsorption was favourable, spontaneous, endothermic physisorption and increased disorder and randomness at the solid- solution interface of MB with the adsorbents. The forward rate constant was much higher than reverse rate constant suggesting dominance of rate of adsorption. PPP was found to be better adsorbent than CPLP.

  20. A comparative study on diurnal changes in metabolite levels in the leaves of three crassulacean acid metabolism (CAM) species, Ananas comosus, Kalanchoë daigremontiana and K. pinnata.

    Science.gov (United States)

    Chen, Li-Song; Lin, Qin; Nose, Akihiro

    2002-02-01

    A comparative study on diurnal changes in metabolite levels associated with crassulacean acid metabolism (CAM) in the leaves of three CAM species, Ananas comosus (pineapple), a hexose-utilizing species, and Kalanchoë daigremontiana and K. pinnata, two starch-utilizing species, were made. All three CAM species showed a typical feature of CAM with nocturnal malate increase. In the two Kalanchoë species, isocitrate levels were higher than citrate levels; the reverse was the case in pineapple. In the two Kalanchoë species, a small nocturnal citrate increase was found and K. daigremontiana showed a small nocturnal isocitrate increase. Glucose 6-phosphate (G-6-P), fructose 6-phosphate (F-6-P) and glucose 1-phosphate (G-1-P) levels in the three CAM species rose rapidly during the first part of the dark period and decreased during the latter part of the dark period. The levels of the metabolites also decreased during the first 3 h of the light period, then, remained little changed through the rest of the light period. Absolute levels of G-6-P, F-6-P and G-1-P were higher in pineapple than in the two Kalanchoë species. Fructose 1,6-bisphosphate (F-1,6-P(2)) levels in the three CAM species increased during the dark period, then dramatically decreased during the first 3 h of the light period and remained unchanged through the rest of the light period. The extent of nocturnal F-1,6-P(2) increase was far greater in the two Kalanchoë species than in pineapple. Absolute levels of F-1,6-P(2) were higher in the two Kalanchoë species than in pineapple, especially during dark period. Diurnal changes in oxaloacetate (OAA), pyruvate (Pyr) and phosphoenolpyruvate (PEP) levels in the three CAM species were similar.

  1. Application of an aqueous two-phase micellar system to extract bromelain from pineapple (Ananas comosus) peel waste and analysis of bromelain stability in cosmetic formulations.

    Science.gov (United States)

    Spir, Lívia Genovez; Ataide, Janaína Artem; De Lencastre Novaes, Letícia Celia; Moriel, Patrícia; Mazzola, Priscila Gava; De Borba Gurpilhares, Daniela; Silveira, Edgar; Pessoa, Adalberto; Tambourgi, Elias Basile

    2015-01-01

    Bromelain is a set of proteolytic enzymes found in pineapple (Ananas comosus) tissues such as stem, fruit and leaves. Because of its proteolytic activity, bromelain has potential applications in the cosmetic, pharmaceutical, and food industries. The present study focused on the recovery of bromelain from pineapple peel by liquid-liquid extraction in aqueous two-phase micellar systems (ATPMS), using Triton X-114 (TX-114) and McIlvaine buffer, in the absence and presence of electrolytes CaCl2 and KI; the cloud points of the generated extraction systems were studied by plotting binodal curves. Based on the cloud points, three temperatures were selected for extraction: 30, 33, and 36°C for systems in the absence of salts; 40, 43, and 46°C in the presence of KI; 24, 27, and 30°C in the presence of CaCl2 . Total protein and enzymatic activities were analyzed to monitor bromelain. Employing the ATPMS chosen for extraction (0.5 M KI with 3% TX-114, at pH 6.0, at 40°C), the bromelain extract stability was assessed after incorporation into three cosmetic bases: an anhydrous gel, a cream, and a cream-gel formulation. The cream-gel formulation presented as the most appropriate base to convey bromelain, and its optimal storage conditions were found to be 4.0 ± 0.5°C. The selected ATPMS enabled the extraction of a biomolecule with high added value from waste lined-up in a cosmetic formulation, allowing for exploration of further cosmetic potential.

  2. Somatic embryogenesis from leaf base callus of Ananas comosus%菠萝叶基愈伤组织诱导体细胞胚

    Institute of Scientific and Technical Information of China (English)

    何业华; 韩景忠; 刘和平; 罗吉; 吴会桃; 王瑞霞; 高爱平; 赵春香; 余小玲; 叶自行; 王泽槐

    2007-01-01

    以菠萝[Ananas comosus(L.)Merr.]吸芽上的叶基部组织为材料,研究了2,4-D、NAA、BA、TDZ在菠萝愈伤组织诱导体细胞胚过程中的影响.结果表明,2,4-D在体细胞胚诱导过程中起着极为重要的作用,其适宜浓度为5mg/L,适当浓度(1 mg/L)的BA在降低畸形胚比例和增加体细胞胚发生率方面有重要作用,但BA也会促进愈伤组织分化不定芽;低浓度的TDZ与BA效果相似,在不同生长调节物质处理组合中,MS+0.01 mg/LTDZ+5 mg/L 2,4-D培养基的体细胞胚发生率最高(97%).低浓度NAA(2~5 mg/L)利于非胚性愈伤组织的增殖,而高浓度的NAA虽能促进体细胞胚发生,但这种体细胞胚很难继续增殖,在萌发时只生根不发芽.成熟体细胞胚在MS+0.2%活性炭上30 d时的转株率为22.7%.

  3. The physiology of ex vitro pineapple (Ananas comosus L. Merr. var MD-2) as CAM or C3 is regulated by the environmental conditions.

    Science.gov (United States)

    Aragón, C; Carvalho, L; González, J; Escalona, M; Amancio, S

    2012-04-01

    Many plant species grown under in vitro controlled conditions can be used as models for the study of physiological processes. Adult pineapple can display CAM physiology while in vitro it functions as a C3 plant. Ex vitro Ananas comosus has plastic morphology and physiology, both easy to modify from C3 to CAM by changing the environmental conditions. The yield of survival for a rentable propagation protocol of pineapple is closely related with the C3/CAM shift and the associated physiological characteristics. In the present work, ex vitro pineapple plants were divided in two sets and subjected to C3 and CAM-inducing environmental conditions, determined by light intensity and relative humidity, respectively, 40 μmol m(-2) s(-1)/85% and 260 μmol m(-2) s(-1)/50%. The results demonstrated that the stress imposed by the environmental conditions switched pineapple plants from C3 to CAM behavior. Comparing to CAM induced, C3-induced pineapple plants showed substandard growth parameters and morphological leaf characteristics but a better rooting process and a higher ABA production, a phenotype closer to adult plants, which are expected to produce fruits in a normal production cycle. We conclude that the upholding of these characteristics is conditioned by low light intensity plus high relative humidity, especially during the first 8 weeks of ex vitro growth. It is expected that the better understanding of pineapple acclimatization will contribute to the design of a protocol to apply as a rentable tool in the pineapple agronomic industry.

  4. On-line gas chromatography combustion/pyrolysis isotope ratio mass spectrometry (HRGC-C/P-IRMS) of pineapple (Ananas comosus L. Merr.) volatiles.

    Science.gov (United States)

    Preston, Christina; Richling, Elke; Elss, Sandra; Appel, Markus; Heckel, Frank; Hartlieb, Ariane; Schreier, Peter

    2003-12-31

    By use of extracts prepared by liquid-liquid separation of the volatiles from self-prepared juices of pineapple fruits (Ananas comosus) (n = 14) as well as commercial pineapple recovery aromas/water phases (n = 3), on-line capillary gas chromatography-isotope ratio mass spectrometry was employed in the combustion (C) and the pyrolysis (P) modes (HRGC-C/P-IRMS) to determine the delta(13)C(VPDB) and delta(2)H(VSMOW) values of selected pineapple flavor constituents. In addition to methyl 2-methylbutanoate 1, ethyl 2-methylbutanoate 2, methyl hexanoate 3, ethyl hexanoate 4, and 2,5-dimethyl-4-methoxy-3[2H]-furanone 5, each originating from the fruit, the delta(13)C(VPDB) and delta(2)H(VSMOW) data of commercial synthetic 1-5 and "natural" (biotechnologically derived) 1-4 were determined. With delta(13)C(VPDB) data of pineapple volatiles 1-4 varying from -12.8 to -24.4 per thousand, the range expected for CAM metabolism was observed. Compound 5 showed higher depletion from -20.9 to -28.6 per thousand. A similar situation was given for the delta(2)H(VSMOW) values of 3-5 from pineapple ranging from -118 to -191 per thousand, whereas 1 and 2 showed higher depleted values from -184 to -263 per thousand. In nearly all cases, analytical differentiation of 1-5 from pineapple and natural as well as synthetic origin was possible. In general, natural and synthetic 1-5 exhibited delta(13)C(VPDB) data ranging from -11.8 to -32.2 per thousand and -22.7 to -35.9 per thousand, respectively. Their delta(2)H(VSMOW) data were in the range from -242 to -323 per thousand and -49 to -163 per thousand, respectively.

  5. Evaluación de Sistemas de Desinfección y Medios para la Micropropagación de la Piña Ananas Comosus L. Merry.

    Directory of Open Access Journals (Sweden)

    Velásquez Yépes Luis Fernando

    1994-09-01

    Full Text Available RESUMEN Se evaluaron diferentes sistemas de desinfección de las yemas de la corona de piña Ananas comosus L. Merry, al igual que algunos medios para la micropropagación, Se ensayaron siete tratamientos de desinfección. Se encontró que el método más satisfactorio (30% de contaminación y 23% de viabilidad de las yemas consistió en el siguiente proceso antes de la siembra: 1. Lavar con agua y detergente las coronas previamente deshojadas, 2. sumergir dichas coronas en una solución de hipoclorito de sodio (NaOCI al 1 % por 5 minutos, 3. enjuagar con agua destilada estéril, 4. extraer las yemas bajo una cámara de flujo laminar, 5. sumergir las yemas en la solución de NaOCL al 1% por 20 minutos 6. enjuagar con agua destilada estéril, 7. desinfección con NaOCl al 0.7%, durante 20 minutos, seguida por tres enjuagues con agua destilada estéril. En la siembra, se ulilizó el medio MS producido por Sigma (1 988, suplementado con 1.0 mg L4 de riamina, piridoxina, ácido nicotínico, sacarosa al 3 % 80 mg L4 de sulfato de ademna. Se incubaron en un cuano a 28°C, 60-65 % de humedad relativa y de 1000 a 3000 lux. En la fase de diferenciación e inducción de yemas axilares se probaron ocho tratamientos, cada uno de 50 subunidades experimentales. Se encontró que los reguladores de crecimiento (ANA y BA favorecieron el desarrollo y la inducción de brotes (26% en dosis de 0,4 mg L4 de ANA y 1,5-2,5 mg L4 de BA. Con el fin de evaluar la consistencia del medio obre el enraizamiento, se tomaron al azar 60 brotes bien desarrollados (3-4 cms y cinco meses de edad con los componentes antes mencionados, excepto Sulfato de adenina. A los dos meses se evaluó el número promedio de raíces y la longitud de éstas. Se encontró que el medio gelificado no propició un mayor enraizamiento.

  6. The Use of Alternate Ligno-cellulosic Raw Materials Banana (Musa sapientum Ankara (Calotropis procera and Pineapple (Ananas comosus in Handmade Paper & their Blending with Waste Paper.

    Directory of Open Access Journals (Sweden)

    Atul Kumar

    2013-09-01

    Full Text Available The studies were made to established suitability of lingo-cellulosic raw materials namely leaf fibreBanana (Musa Sapientum, bast fibre Ankara (Calotropis Procera, & leaf fibre Pineapple (Ananas Comosus for making pulps for handmade paper industry. This should help in providing a cost effective, good quality cellulosic raw material as an alternate to cost prohibitive traditionally used cotton hosiery waste traditionally used for manufacturing good quality handmade paper & it’s products. This will help in improving the cost economics & competitiveness of the Indian paper industry in the global market besides addressing the problems of environment & global warming. The aim of the research was to study the extraction, morphology, chemical composition and pulping of these fibres and relate these properties to the composite properties obtained with these fibres as reinforcement with short fibres viz. waste paper. For the pulp production to be feasible it is essential to use suitable pulping methods, which maximize the yield of pulp and introduce as low damage as possible to the fibres. The different pulping methods were applied to these fibre to get optimized strength properties papers. The Studies thus carried out provide useful information about the nature of these raw materials, suitable pulping & bleaching process to produce an eco-friendly handmade paper and converted products. The research work provides a good quality cost effective lingo-cellulosic raw material for handmade paper industries with a possibility of replacement of the expensive and traditionally used cotton hosiery waste.The paper thus produced using environmental friendly pulping and bleaching process is characterized for its strength properties like tensile, tear, bursting, folding endurance and other parameters. The effluents generated from pulping and bleaching of above ligno-cellulosic waste materials were characterized for various pollution parameters like Residual Alkali

  7. Adubação foliar com macro e micronutrientes no crescimento de mudas micropropagadas do abacaxizeiro cv. Gold [Ananas comosus (L. Merrill] em diferentes recipientes Foliar fertilization with macro and micronutrients in the growth of plantlets micropropagated of pineapple cv. Gold [Ananas comosus (L. Merrill] in different containers

    Directory of Open Access Journals (Sweden)

    Izaias dos Santos Bregonci

    2008-06-01

    Full Text Available Objetivou-se com este trabalho avaliar o efeito da adubação foliar com macro e micronutrientes no crescimento das mudas micropropagadas do abacaxizeiro cv. Gold [Ananas comosus (L. Merrill], em diferentes recipientes. O experimento foi em esquema fatorial 8x3, adubação foliar em 8 níveis e recipientes em 3 níveis, através de um delineamento inteiramente casualizado com 5 repetições. As mudas foram padronizadas com altura média de 7,12 cm. As adubações foliares foram feitas com uréia, cloreto de potássio, ácido bórico, um formulado comercial com macro e micronutrientes e testemunha (pulverização com água e os recipientes: bandeja de isopor com 200 células; tubete pequeno de 115 cm³; e tubete grande com 300 cm³. O substrato utilizado foi o plantmax hortaliças®. Avaliaram-se as características área foliar, altura de planta e massa seca da parte aérea e da raiz, aos 140 dias do transplantio. Os adubos foliares proporcionaram maior crescimento em área foliar, altura e massa seca da parte aérea às mudas do abacaxizeiro, embora com resultados diferentes. Os adubos foliares não aumentaram a massa seca do sistema radicular. A bandeja de isopor apresentou as menores médias, com todos os adubos foliares para área foliar, altura e massa seca da parte aérea das mudas do abacaxizeiro. O tubete pequeno e o tubete grande apresentaram resultados semelhantes com a maioria dos adubos foliares utilizados.The objective of this work was to evaluate the effect of the foliar fertilization with macro and micronutrients on the growth of the plantlets micropropagated of pineapple cv. Gold [Ananas comosus (L. Merrill] in different containers. The experiment was mounted in factorial arrangement 8x3, with foliar fertilization in 8 levels and containers in 3 levels, through a completely randomized design with five repetitions. The plantlets were standardized with average height of 7,12 cm. The foliar fertilization was used in the urea

  8. Cultivo inicial in vitro de gemas axilares de Ananas comosus (L. Merr., em meio líquido/sólido, na presença/ausência de luz In nitial in vitro culture of axillary buds of Ananas comosus (L. Merr. in liquid/semi-solid culture medium, in the presence/absence of light

    Directory of Open Access Journals (Sweden)

    Sarah Brandão Santa Cruz Barboza

    2009-01-01

    Full Text Available A capacidade regenerativa de gemas axilares de abacaxizeiro cv. Pérola (Ananas comosus (L. Merr. foi avaliada em meio de cultivo líquido e gelificado, na presença e ausência de luz, com o objetivo de produzir material para subsequentes fases da micropropagação. Os explantes foram estabelecidos, por 60 dias, em meio MS acrescido de 0,63 μM de ANA e 2,5 μM de BAP. O delineamento experimental foi inteiramente casualizado, constituído com quatro tratamentos (meio líquido com e sem ponte de papel de filtro e meio gelificado com ágar a 6,0 g L-1 e com Phytagel® a 3,0 g L-1, com quatro repetições e cinco explantes por repetição. Diferentes volumes de meio líquido (2,5 mL; 5,0 mL e 10,0 mL também foram avaliados, usando-se quatro repetições por volume de meio de cultura. Maior número de brotos foi obtido na presença de luz, em meio líquido sem ponte de papel de filtro, onde 80% dos explantes se diferenciaram em brotos. Na ausência de luz, 68,8% a 87,5% dos explantes não regeneraram brotos. Explantes cultivados em volumes de 5,0 mL ou 2,5 mL de meio MS líquido, acrescido de 0,63 μM de ANA e 2,5 μM de BAP, na presença de luz, constitui um protocolo adequado para cultivo inicial de gemas axilares e regeneração de brotos de abacaxizeiro.The regenerative capacity of buds from lateral sprouts of the pineapple cv. Pérola (Ananas comosus (L. Merr. was evaluated in liquid and semi-solid medium, under light and dark conditions, with the objective of producing materials for subsequent phase of micropropagation. The explants were established for 60 days in basal MS medium, supplemented with 0.63 μM NAA and 2.5 μM BAP. The statistical design was completely randomized with four treatments (liquid medium with and without filter paper bridge, and semi-solid medium gellified with agar and phytagel®, four repetitions and five explants per experimental unit. Different volumes of liquid medium (2.5 mL, 5.0 mL, and 10.0 mL were also

  9. In vitro and in vivo evaluation of the activity of pineapple (Ananas comosus) on Haemonchus contortus in Santa Inês sheep.

    Science.gov (United States)

    Domingues, Luciana Ferreira; Giglioti, Rodrigo; Feitosa, Karina Alves; Fantatto, Rafaela Regina; Rabelo, Márcio Dias; de Sena Oliveira, Márcia Cristina; Bechara, Gervásio Henrique; de Oliveira, Gilson Pereira; Barioni Junior, Waldomiro; de Souza Chagas, Ana Carolina

    2013-10-18

    The development of resistance to anthelmintics has prompted research into alternative methods of controlling intestinal nematodes in ruminants. This study aimed to assess the activity of Ananas comosus on Haemonchus contortus in Santa Inês sheep. The aqueous extract of pineapple skin (AEPS), bromelain from pineapple stems (B4882) and residue from pineapple processing was evaluated in in vitro and in vivo tests. The enzymatic activity of substances was analyzed by the azocasein method. The egg hatch test (EHT) and larval development test (LDT) were performed using the Embrapa2010 isolate of H. contortus. In the in vivo test, 36 sheep artificially infected with H. contortus were divided into six groups: G1: 2 g/kg BW of the aqueous extract administered for three days; G2: 2 g/kg BW of the industrial pineapple residue for 60 days; G3: 180 mg/animal of bromelain in a single dose; G4: negative control I; G5: positive control (levamisole phosphate); and G6: negative control II. The eggs per gram (EPG) in the feces were counted till 28 days after treatment. LC₅₀ and LC₉₀ were obtained by the probit procedure, while the in vivo test results were analyzed by GLM. The aqueous extract in the in vitro and in vivo test, the bromelain and industrial residue presented 0.102, 0.157, 1.864 and 0.048 enzyme units/mL, respectively. In the egg hatch test, the LC₅₀ and LC₉₀ were respectively 31 and 81 mg/mL for the aqueous extract and 0.50 and 2 mg/mL for bromelain. In the larval development test, the LC₅₀ and LC₉₀ were respectively 1.7 and 7.3 mg/mL for the aqueous extract and 0.019 and 0.086 mg/mL for bromelain. In the in vivo test, the general efficacies of the treatments in relation to the negative control were 22.6%, 42.2%, 3.65% and 89% for the aqueous extract, industrial pineapple residue, bromelain and positive control respectively. The transformed EPG values were 3.19 ± 0.59, 3.32 ± 0.25, 2.85 ± 0.66, 3.44 ± 0.50, 2.28 ± 0.93 and 2.75 ± 0.94 for

  10. CYPs Induction of Polyphenol Extract from New Medicinal Resource Ananas Comosus Leaf on Mice%新药用资源菠萝叶对小鼠相关药物代谢酶的诱导作用

    Institute of Scientific and Technical Information of China (English)

    姜敬非; 邢东明; 杜力军; 王玉刚; 胡珺; 冯天师; 卢希; 袁梽漪; 詹宏磊; 李慧玉; 雷帆

    2012-01-01

    目的:观察菠萝叶对小鼠肝脏药物代谢酶CYP1A2、2E1和3A11的诱导表达及其活性作用,为药物相互作用提供实验依据.方法:采用整体动物实验方法,灌胃给予不同剂量的菠萝叶酚,取肝脏做mRNA和蛋白的表达.同时制备粗酶液,以相应的特异性底物探针观察相关酶活的影响.结果:小鼠连续灌胃给药3天,对CYP 2E1的mRNA表达有一定的诱导作用,但对蛋白表达及其活性没有明显影响;对CYP 1A2活性有一定的诱导作用,但对mRNA及蛋白无明显影响;对CYP 3A11的诱导作用不明显.结论:菠萝叶酚对CYP 1A2和2E1部分指标有一定的影响,表明其作用具有一定的复杂性,尚需进一步深入研究.%This study was aimed to determine the induction of polyphenol extract from Ananas comosus leaf, which is a new medicinal resource, on CYP1A2, 2E1 and 3A11 in order to present data for possible interaction between Ananas comosus leaf and other drugs. After oral administration of the extract for 3 days, the liver was removed for the detection of CYP1A2, 2E1 and 3A11. Expressions of mRNA (qPCR assay) and protein (Western blot assay) were carried out. Phenacetin (CYP 1A2), chlorzoxazone (CYP 2E1) and erythromycin (CYP 3A11) were employed for the enzyme activity of CYP 1A2, CYP 2E1 and CYP 3A11, respectively. It was shown that the extract promoted the mRNA expression of CYP 2E1, enhanced the activity of CYP 1A2. These complex effects need to be studied further.

  11. Efficacité de la technique d'induction florale d'Ananas comosus (L. Merr. au moyen de charbon actif enrichi à l'éthylène (TIFBio

    Directory of Open Access Journals (Sweden)

    Lebeau F.

    2009-01-01

    Full Text Available Efficiency of the Ananas comosus (L. Merr. flower induction treatment based on ethylene enriched activated carbon (TIFBio. Pineapple is an important export crop for tropical countries. The flower induction treatments are essential to the pineapple production for economical and social reasons. For conventional agriculture, many chemicals are available but for organic farming ethylene is the only allowed product. A new flower induction method suited to small organic growers has been developed by the Pesticides Initiative Programme of the Coleacp funded by the European Development Fund. The trials conducted proved that the method reaches more than 80% efficiency at 10 weeks for the different application methods evaluated. The wet application trials show a doses response effect as well as effect of the application replication at 2 days interval. The flowering rate culminates at 100% after 8 weeks for the best results, obtained with the wet treatment at 250 mg per plant applied two times at 2 days interval. The different dry treatments tested gave all 85 – 90% flowering rates at 10 weeks, suggesting the presence of an undetermined limitation factor in the conditions prevailing for the trial. The time of application during the day shows no significant effect. It is concluded that the growers can use the TIFBio technique for production control. It is recommended to evaluate the most suited application technique according to their particular case as environmental effect can affect the efficiency.

  12. Effects of Different Pretreatments on Micorganism of Fresh-cut Ananas comosus%预处理方法对鲜切菠萝微生物的影响

    Institute of Scientific and Technical Information of China (English)

    邵远志; 李雪萍; 贾文君; 李雯

    2011-01-01

    为保持和改善鲜切菠萝的贮藏品质,以‘巴厘’菠萝为试材,研究不同预处理方法(对照,有机酸,漂烫和有机酸+漂烫)对鲜切菠萝微生物的影响.结果表明:各处理的大肠菌群发生较少且总数极低;在对霉菌的影响上,漂烫处理的抑制效果最明显,至贮藏末期,其霉菌总数仅为CK的1/10,其次是综合处理;鲜切菠萝的酵母菌含量随着贮藏时间的延长不断增多,对照处理上升最快,其次是有机酸处理,而漂烫处理和综合处理的增加缓慢,至贮藏末期(11天),分别比对照低1.7× 106 cfu/g和1.5×106 cfu/g,处理间差异极显著(P<0.01);鲜切菠萝的菌落总数不断增加,对照处理上升最快,其次是有机酸处理,漂烫处理的增加最慢.说明霉菌、酵母菌是鲜切菠萝的主要微生物,漂烫处理能够较显著地抑制鲜切菠萝霉菌、酵母菌和菌落总数的上升,防止果实腐烂变质,而对照和单独酸处理在鲜切菠萝品质保持方面的效果较差.%In order to maintain and improve the storage quality of fresh-cut Ananas comosus, Ananas comosus 'Bali' was used to investigate the effects of different pretreatments (control, organic acid, blanching and organic acid combined with blanching) on micorganism during storage. The results showed that there were fewer and lower number of coliform bacteria in storage of fresh-cut pineapple; for the impact of mold, the effect of blanching was the most obvious, the number of fungus was only 1/.10 of control when the day 5, followed by the integrated treatment. With the extension of storage time, the yeast content of fresh-cut pineapple increased, the yeast increasing of control treatment was the fastest, followed by organic acid treatment, while, the yeast increasing of blanching and integrated treatments were most slowly, at the end of storage (11 d), the yeast contents of blanching and integrated treatments were lower 1.7xl06cfu/g and 1.5xl06cfu/g than the

  13. 菠萝果实发育过程中糖积累与其代谢酶的关系%Sugar Accumulation and Changes in Activities of Sucrose-Metabolizing Enzymes of Pineapple (Ananas comosus(L) Merr.)

    Institute of Scientific and Technical Information of China (English)

    张秀梅; 杜丽清; 孙光明; 陈佳瑛; 弓德强; 李伟才; 谢江辉

    2008-01-01

    以菠萝[Ananas comosus(L.)Merr.]"巴厘"和"台农11号"两个品种为试验材料,测定不同发育时期果实中蔗糖、葡萄糖和果糖含量以及蔗糖代谢相关酶--酸性转化酶(AI)、中性转化酶(NI)、蔗糖合成酶(SS)和蔗糖磷酸合成酶(SPS)的活性,并对果实中糖积累与酶活性的关系进行分析.结果表明:2个品种果实中糖积累动态十分相似,即果实发育前期,糖积累缓慢;进入成熟期,蔗糖迅速积累,而己糖"巴厘"果实中前期变化较少,后期稍有积累,"台农11号"果实中前期稍有积累,后期基本不变.2个品种果实中蔗糖含量与SS和SPS活性相关系数分别达到正的显著或极显著水平,NI活性与2个品种果实中蔗糖含量呈极显著负相关,蔗糖含量与AJ的活性相关性品种间差异明显.

  14. Histocytology Observation on the Somatic Embryogenesis in Ananas comosus Callus%菠萝愈伤组织中体细胞胚起源过程的组织细胞学观察

    Institute of Scientific and Technical Information of China (English)

    何业华; 方少秋; 马均; 胡中沂; 卢敏; 彭兵

    2010-01-01

    以菠萝(Ananas comosus)'神湾'品种为材料,采用石蜡切片法对其愈伤组织中球形胚形成过程进行了组织细胞学观察.结果表明,非胚性愈伤组织转入到MS+4.0 mg·L-12,4-D+0.5 mg.L-16-BA培养基培养后,愈伤组织中先出现一些胚性细胞;然后单个胚性细胞分裂形成一个由多细胞组成的原胚.原胚细胞分生能力强,与周围的母体组织之间形态差异显著,界限明显,其下部形成一个类似胚柄的结构.随着原胚不断生长,其形状由长椭圆形发展成倒卵形,最后逐渐形成表面较光滑、外观近球形的球形胚,并在'胚柄'基部与母体组织连接处形成离层.与此同时,球形胚内出现细胞分化.菠萝体细胞胚发生具有内、外两种起源方式,另外愈伤组织中体细胞胚发生普遍存在着不均匀、不同步和次生胚等现象.

  15. 乙烯利处理对菠萝果实生长发育和品质的影响%Effects of Ethephon on Fruit Development of Pineapple[Ananas comosus(L.)Merr.

    Institute of Scientific and Technical Information of China (English)

    庞观胜; 袁晓丽; 杜丽清

    2013-01-01

    [Objective] The aim was to study the effects of applying ethephon in early fruit development on fruit growth and development and quality of pineapple.[Method] Every pineapple plant was sprayed 40 μg/L ethephon on the leaf,and then determined its single fruit weight,fruit's transverse diameter and vertical diameter,soluble solids content,Vc content,soluble sugar content,and organic acids content.[Result]40 μg/L ethephon liquid applying after flowering on the leaf had a promoting effect on fruit' s transverse diameter and vertical diameter,single fruit weight,soluble sugar content,and soluble solids content in a degree,while had opposite effect on titratable acidity content.[Conclusion]The study provides a reference for the scientific application of ethephon.%[目的]研究果实发育前期喷施低浓度乙烯利对菠萝[Ananas comosus(L.)Merr.]果实生长发育和品质的影响.[方法]每株叶面喷施40 μg/L乙烯利溶液,测量菠萝果实的单果重、横径、纵径、可溶性固形物含量、VC含量、可溶性糖含量、有机酸含量.[结果]在花后喷施40μg/L乙烯利溶液,对果实的纵径、横径、单果重、可溶性糖含量、可溶性固形物含量都有不同程度的促进作用,对可滴定酸含量有不同程度的降低作用.[结论]该研究可为科学合理地使用乙烯提供指导.

  16. Establishment of Direct Somatic Embryogenesis System Through Transverse Thin Cell Layer Culture in Pineapple (Ananas comosus L. Merrill)%从菠萝薄细胞层切片直接诱导体细胞胚

    Institute of Scientific and Technical Information of China (English)

    梁雪莲; 陈晓玲; Kheng Cheah; Diane M Sether; Qi Li; John Hu

    2009-01-01

    以菠萝(Ananas comosus L.Merrill)杂交种MD1无菌苗为材料,通过培养薄细胞层切片(Thin cell layer,TCL)的方法,研究直接再生胚的固体培养体系.结果证明,预培养的设计2(在预培养基PT上黑暗预培养无菌苗1周后,从预培养苗茎顶端切取TCL,在MS+2,4-D 2 mg·L~(-1)或1 mg·L~(-1)胚诱导培养基EI2上黑暗培养1周,然后转到光照条件下继续诱导胚1周,再在成熟胚培养基EM上培养2周,最后转入幼苗培养基PL上培养)是直接诱导获得体胚的最佳途径;诱导的体胚最初均来自于薄细胞层上微管束附近的细胞;picloram有利于芽的诱导,2,4-D有利于直接再生胚,但如果球形胚形成后一直培养在较高浓度的2,4-D培养基中,将阻碍球形胚和心形胚进一步发育为成熟胚,反而促进芽的生成.%The direct embryogenesis in vitro using transverse thin cell layer (TCL) culture system of hybrid pineapple (MDI) seedling was established. By comparison of 3 protocols, the effect of pretreatment on inducing direct embryo, the functional difference between 2, 4-D and picloram for inducing morphogenesis and the original cell of directly induced embryo in solid culture system were studied. The results revealed that protocol 2 in which involved pretreatment is the best and all the direct somatic embryos originated from cells neat the vascular bundles on TCL. Relatively, 2, 4-D was good for direct embryo inducing, while plcloram was good for shoot inducing. But, if the globe and heart embryos were continuously exposed to higher 2, 4-D,development of them into mature embryos was inhibited and shoot development of them was promoted.

  17. Agrobacterium-mediated transformation of Ananas comosus with CYP1A1%根癌农杆菌介导CYP1A1转化菠萝的研究

    Institute of Scientific and Technical Information of China (English)

    何业华; 吴会桃; 罗吉; 方少秋; 马均; 卢敏; 彭兵; 伍成厚

    2010-01-01

    将菠萝(Ananas comosus)愈伤组织经含有植物表达重组质粒(pUHA1-CYP1A1)的根癌农杆菌(LBA4404菌株)侵染后,在MS+3.0 mg/L BA+2.0 mg/L NAA+100 μmol/L AS+8 g/L agar上共培养3 d,转入选择培养基(MS+3.0 mg/L BA+2.0 mg/L NAA+20 mg/L Km+400 mg/L Carb+8 g/L agar)上培养约10 d后开始分化出不定芽;28 d后将绿色的抗Km不定芽转入MS+2.0 mg/L NAA+30 mg/L Km+300 mg/L Carb+8 g/L agar上再进行连续2轮选择,随后将绿芽转入MS+1.0 mg/L IBA+30 mg/L Km+8 g/L agar上生根,共获得95株Km抗性植株,转化率0.12%~2.69%.对其中部分的抗Km植株进行PCR检测,PCR阳性植株率达64.29%.经Southern杂交进一步证实,CYP1A1已整合到菠萝基因中.以琼脂为培养基凝固剂、共培养基中添加AS、增加选择次数和逐渐增加新一轮选择培养基中的Km质量浓度等是根癌农杆菌介导菠萝愈伤组织获得转基因植株的重要条件.

  18. 菠萝非胚性愈伤组织的类型及其器官发生%Classification and organogenesis of non-embryogenic callus from Ananas comosus

    Institute of Scientific and Technical Information of China (English)

    何业华; 罗吉; 胡中沂; 王瑞霞; 高爱平; 方少秋; 赵春香; 杨向晖; 叶自行; 王泽槐

    2008-01-01

    以菠萝[Ananas comosus(L.)Merr.]非胚性愈伤组织为材料,利用离体培养及组织细胞学等方法研究了其在增殖、分化过程中的特性和再生规律.在增殖培养基上经过3次以上继代培养后,非胚性愈伤组织形态上逐渐表现出一定差异.根据其形态结构、分化能力和组织细胞学特征,可把非胚性愈伤组织分为节瘤状组织、薄壁组织团、致密深层愈伤组织和表面疏松愈伤组织4种类型;在增殖培养基MS+3.0 mg/L BA+2.0 mg/L NAA上,一种类型的非胚性愈伤组织通常只会生长出同类型的非胚性愈伤组织,但通过改变培养基配方等方法可使不同类型之间发生转换.同时,增殖继代过程中的器官发生现象很常见,通常是在薄壁愈伤组织表面细胞分化成具有分生能力的细胞,然后进行不定芽的分化.4类非胚性愈伤组织都可以通过拟原球茎、多芽体以及不定芽的形式进行器官发生;也可以经胚性诱导由体细胞胚发生途径再生成植株,其中拟原球茎产生体细胞胚的能力最强,多芽体其次.生长素及细胞分裂素对非胚性愈伤组织的形态结构、分化途径以及增殖能力有很大的影响.

  19. Chemical constituents from leaves of Ananas comosus and their biological activities%菠萝叶的化学成分及生物活性研究

    Institute of Scientific and Technical Information of China (English)

    黄筱娟; 陈文豪; 纪明慧; 郭飞燕; 舒火明; 郑彩娟

    2015-01-01

    目的 研究菠萝Ananas comosus叶的化学成分及生物活性.方法 采用硅胶柱色谱、Sephadex LH-20柱色谱、重结晶等方法进行分离纯化,根据理化性质和波谱数据鉴定化合物结构,并对各化合物进行抗菌活性、卤虫致死活性测定.结果 从菠萝叶95%乙醇提取物中分离纯化得到8个化合物,分别鉴定为tricin-4'’-O-[10"-O-(8"-hydroxyl) feruloyl-(9"'-O-p-coumaroyl) glyceryl] ether (1)、2,4-二氯苯甲酸(2)、小麦黄素(3)、金圣草黄素(4)、1-O-对羟基肉桂酰单甘油酯(5)、1-O-阿魏酰单甘油酯(6)、1-O-阿魏酸-3-O-对羟基肉桂酰甘油酯(7)、1,3-二氧阿魏酰甘油酯(8).化合物1对金黄色葡萄球菌和大肠杆菌表现出与阳性对照环丙沙星相当的抑制活性,最小抑菌浓度(MIC)均达到0.156 μg/mL.卤虫致死活性结果显示,化合物1和4的ICso值分别为21.4、25.0 μtg/mL.结论 除化合物5外,其余化合物均首次从该植物中分离得到,其中化合物1为新化合物,命名为菠萝叶酯A,且有良好的抑菌活性和卤虫致死活性.

  20. Cloning and expression analysis of MADS-box gene AcMADS2 from pineapple (Ananas comosus)%菠萝MADS-box基因AcMADS2的克隆与表达分析

    Institute of Scientific and Technical Information of China (English)

    杨祥燕; 蔡元保; 张治礼; 孙光明; 孙伟生; 吴青松; 王运儒

    2016-01-01

    A new cDNA sequence encoding MADS-box protein, designated as AcMADS2 (GenBank accession number: KC257409), fromAnanas comosus was cloned by RT-PCR and RACE techniques. The full length cDNA ofAcMADS2 has 945 bp nucleotides with an ORF of 687 bp encoding a precursor protein of 228 amino acid residues. Sequence analysis and evolution analysis indicated that the deduced protein contained conserva-tive MADS-box domain and semi-conservative K domain, and shared highly homology to AGL12clade of MADS-box proteins from other plants. Bioinformatics analysis results demonstrated that AcMADS2 was an unstable and hydrophilic protein without transmembrane region and signal peptide; α-helix, extended strand and random coil were the backbone of its secondary structure; the protein core structure with MADS-box do-main in predictable protein 3D model; and might be located in the nucleus as a transcription factor. Real-time quantitative PCR analysis showed that the expression of AcMADS2 gene was much stronger in roots than in lfo-rets, stems and leaves at lfowering stage, and the adversity stresses such as low temperature and drought could trigger a signiifcant induction ofAcMADS2 gene in roots at seedling stage. All these results suggested that the AcMADS2protein may be involved in the regulation of root development, and play an important role in re-sponses to adversity stresses.%采用RT-PCR和RACE方法从菠萝克隆获得一个MADS-box基因,命名为AcMADS2, GenBank登录号为KC257409。Ac-MADS2基因cDNA全长945 bp, ORF为687 bp,编码一个含有228个氨基酸的蛋白。序列分析和系统进化分析表明, Ac-MADS2具有保守的MADS-box结构域和半保守的K区,与其他植物中的AGL12亚家族MADS-box蛋白具有很高的同源性。生物信息学分析结果表明, AcMADS2是不稳定的亲水蛋白,不具有跨膜结构和信号肽。α-螺旋、折叠延伸链及无规则卷曲构成了二级结构的主要蛋白框架。在三级结构中MADS-box结构

  1. Antibioticoterapia em microplantas de abacaxizeiro (Ananas comosus

    Directory of Open Access Journals (Sweden)

    Gabriela Ferraz Leone

    2016-01-01

    Full Text Available RESUMO: Com o intuito de avaliar os efeitos da antibioticoterapia no crescimento de plantas micropropagadas de abacaxizeiros, bem como na comunidade bacteriana endofítica da espécie, realizaram-se análises moleculares. Verificou-se a presença de endófitos no interior das microplantas, mesmo após 30 dias de cultivo em meio de cultura MS, suplementado com diferentes antibióticos, e analisou-se o crescimento da parte aérea e radicular das microplantas. Decorrido o período de 30 dias de cultivo, constatou-se ainda a presença de bactérias endofíticas, no entanto, as microplantas apresentaram acentuada redução no crescimento da parte aérea e radicular, evidenciando assim a influência da microbiota endofítica no crescimento das microplantas in vitro e a importância da manutenção do equilíbrio endofítos/microplanta.

  2. Diurnal Changes in the Contents of Adenine Nucleotides and Inorganic Pyrophosphate in the Chlorenchyma and the Water Storage Parenchyma of Ananas comosus Leaves%菠萝叶片绿色组织与贮水组织的腺苷酸及焦磷酸水平的昼夜变化

    Institute of Scientific and Technical Information of China (English)

    陈立松; 齐一萍; 等

    2001-01-01

    研究了景天酸代谢(CAM)植物菠萝(Ananas comosus)叶片绿色组织与贮水组织的苹果酸、腺苷酸及焦磷酸含量的昼夜变化。夜间苹果酸的积累仅发生在绿色组织中,而且,其含量也远高于贮水组织。绿色组织中能荷和无机磷含量夜间增高,白天下降。绿色组织中焦磷酸含量夜间增加,在白天的头几个小时迅速下降到低的水平,然后保持稳定。与绿色组织相比,贮水组织中ATP、ADP、无机磷和焦磷酸的含量低得多,且不表现昼夜变化,在贮水组织中没有测到AMP。

  3. Evaluación económica de la aplicación de los procesos tecnológicos de deshidratación Osmótica y por Flujo de Aire Caliente de la piña (Ananas Comosus variedad Cayena lisa

    Directory of Open Access Journals (Sweden)

    Annia García Pereira

    2013-01-01

    Full Text Available La aplicación de métodos de conservación se reconoce como una variante efectiva para incrementar el aprovechamiento y mejor utilización de las producciones agrícolas. El presente trabajo tiene como objetivo evaluar a través de indicadores económicos la aplicación de la deshidratación Osmótica y por Flujo de Aire Caliente de la Piña (Ananas Comosus, variedad Cayena lisa. Durante el análisis se utiliza la metodología propuesta en la NC 34-38:2001 con algunas modificaciones. Para el procesamiento de los datos se utiliza la herramienta Excel de Microsoft office 7. Como resultado se obtuvo que los gastos totales del proceso ascienden a un monto de 49.07 pesos para la DO y 15.03 pesos para la DAC para procesar 550 g en cada proceso.

  4. Fruitone CPA para retardar la maduración en piña Ananas comosus (L. Merr., cv. Cayena Lisa, cosechada en primavera Fruitone CPA for delay the fruit maturity of pineapple Ananas comosus (L Merr. cv. Smooth Cayenne harvested in spring time

    Directory of Open Access Journals (Sweden)

    ANDRÉS REBOLLEDO-MARTÍNEZ

    2002-08-01

    Full Text Available El objetivo fue determinar el efecto de dosis y número de aplicaciones de Fruitone CPA (ácido 2-3 clorofenoxi-propiónico en el retraso de la maduración, rendimiento y calidad del fruto de piña en el periodo de primavera. El experimento se llevó al cabo en el Campo Experimental Papaloapan, del INIFAP, en Veracruz, México. Se estableció con el cv. Cayena Lisa de México, en un diseño de bloques al azar con cuatro repeticiones; los tratamientos fueron nueve, incluido el testigo sin aplicación, 300, 600, 900 y 1200 mL por hectárea en una aplicación y 600, 900, 1500 y 2100 mL por hectárea divididas en dos aplicaciones iguales, espaciadas ocho días, de Fruitone CPA; los tratamientos se establecieron a los 135 días después de la inducción floral, a 65 días antes de la cosecha del testigo. Los resultados muestran un alto y significativo efecto del Fruitone en el peso de la fruta, aunque no hubo diferencias entre el número de aplicaciones; el contenido de ácido cítrico y los sólidos solubles totales se incrementaron con la aplicación del Fruitone. No se presentaron frutos agrietados ni con Mancha Café interna en ningún tratamiento. El retraso de la cosecha con respecto al testigo varió desde los seis hasta los 21 días, en función de la dosis. Se concluye que el mejor tratamiento fue el de 900 mL de Fruitone CPA por hectárea, en una sola aplicación, el cual incrementó el peso del fruto en un 14% y retrasó la cosecha 19 días.The objective of this research was to evaluate the effect of rates and applications of Fruitone CPA (acid 2-3 clorofenoxi -propionic over maturity delay, yield and quality of the pineapple fruit in spring time. The experiment was carried out in the Campo Experimental Papaloapan (INIFAP, in Veracruz State, Mexico. It was established with the cv. Smooth Cayenne, in randomized blocks, design with four replicates; totalizing nine treatments including, the control: 300, 600, 900 and 1200 mL of Fruitone CPA

  5. Determinação de proteína total e escore de aminoácidos de bromelinas extraídas dos resíduos do abacaxizeiro (Ananas comosus, (L. Merril Determination of total protein and aminoacid composition of bromelain extracted from pineapple plant residues (Ananas comosus, (L. Merril

    Directory of Open Access Journals (Sweden)

    Lenice O. FREIMAN

    1999-05-01

    Full Text Available O abacaxi por longo tempo tem sido a fruta não cítrica mais popular nos países tropicais e sub-tropicais, principalmente pelo seu atrativo sabor e aroma, além do refrescante balanço açúcar-ácido que possui, o que certamente, determinou sua importância sócio-econômica. Além dessas características, é fonte da enzima proteolítica bromelina, um produto de alto valor comercial, que não é fabricado no Brasil. O presente trabalho objetivou a determinação do escore de aminoácidos de bromelinas extraídas das várias partes do abacaxizeiro (casca, folha, caule, coroa e polpa, com duas precipitações subseqüentes, utilizando-se como agente precipitante, o etanol p.a. Análises mostraram maior teor de proteínas na bromelina extraída da coroa. Nas duas precipitações a composição de aminoácidos das enzimas obtidas, apresentaram resultados inferiores aos da comercial.For a long time pineapple has been most popular noncitric fruit in tropical and subtropical countries, principally for its attractive flavor and aroma, besides refreshing sugar-acid balance, what certainly determined its social-economic importance. In addition to these characters, it is a source of the proteolytic bromelain enzyme , that is a high valued commercial product not made in Brazil. The present research has aimed in determination the aminoacids composition of extracted bromelain from some parts of the pineapple plant ( peel, leaf, stem, garland and pulp with two subseqüents precipitation’s using ethanol p.a. as precipitating agent. Analysis showed higher protein content in extracted bromelain from the garland. In the two precipitation’s, the aminoacids composition from the obtained enzymes presented lower results than the one from the commercial enzyme.

  6. Cloning of Flowering-related Gene AcMADS1 and Characterization of Expression in Tissues of Pineapple (Ananas comosus)%菠萝花发育相关基因AcMADS1的克隆与组织表达特性分析

    Institute of Scientific and Technical Information of China (English)

    蔡元保; 杨祥燕; 孙光明; 黄强; 刘业强; 李绍鹏; 张治礼

    2014-01-01

    MADS-box转录因子在植物的发育过程特别是控制花器官的诱导与发育中起关键作用.利用同源克隆结合RACE技术,从菠萝(Ananas comosus)花中分离出1个新的菠萝MADS-box基因,命名为AcMADS1(GenBank登录号为KC257408).AcMADS1基因的编码区为726 bp,编码241个氨基酸,蛋白质分子量为27.50 kDa,等电点为9.26.序列比对和系统进化树分析表明,AcMADS1具有保守的MADS-box及半保守的K区,属于AGL6亚家族MADS-box蛋白.生物信息学分析表明,AcMADS1是亲水碱性蛋白,二级结构主要以α-螺旋、无规则卷曲和折叠延伸链为蛋白质骨架,三级结构中蛋白核心结构符合转录因子与DNA结合的常见功能域MADS-box,而且作为转录因子定位于细胞核中.组织特异性表达分析表明,AcMADS1基因在菠萝果肉以及花器官的雌蕊、花瓣和萼片中均有表达,但在雄蕊以及营养器官的根、茎和叶中几乎不表达;且在花器官早期发育过程中大量表达,后期呈下降趋势.因此推测这个基因可能在菠萝花器官发育和开花诱导过程中起重要作用.

  7. 菠萝叶片绿色组织与贮水组织液泡膜ATPase和焦磷酸酶特性%Characteristics of Tonoplast Adenosinetriphosphatase and Inorganic Py-rophosphatase in the Chlorenchyma and the Water Storage Parenchyma of Ananas comosus Leaves

    Institute of Scientific and Technical Information of China (English)

    陈立松; NOSEAkihiro; 等

    2002-01-01

    研究了景天酸代谢(CAM)植物菠萝叶片绿色组织与贮水组织液泡膜AT Pase和焦磷酸酶(PPase)特性.绿色组织有较高的ATPase和PPase活力.贮水组织(绿色组织 )ATPase和PPase活力的最佳温度分别为43和49℃(37和46℃).当温度小于最佳温度时,A TPase和PPase活力随着温度的上升而上升;而当温度大于最佳温度时,ATPase和PPase活力 随着温度的上升而下降.在(10~46℃)10~37℃的温度范围,绿色组织(PPase)ATPase活 力随着温度的上升而上升的速度大于贮水组织中(PPase)ATPase活力上升的速度,绿色组 织有(相对)较高的ATPase和PPase活力;但在(49~61℃)43~55℃的温度范围,贮水组织 中(PPase)ATPase活力随着温度的上升而下降的速度小于绿色组织中(PPase)ATPase活力 下降的速度,贮水组织有相对较高的ATPase和PPase活力.此外,贮水组织中这两种酶活力 的热稳定性强于绿色组织中这两种酶活力的热稳定性.对这两种酶活力的最佳pH和底物依赖性也进行了研究.%The characteristics of tonoplast adenosinetri hosphatase(ATPase) and inorganic pyrophosphatase(Ppase) in the chlorenchyma and the water storage parenchyma(WSP) of crassulacean acid metabolism(CAM) plant Ananas comosus(pineapple) leaves were investigated. The activities of tonoplas t ATPase and Ppase in the chlorenchyma were higher than those in the WSP. The op timum temperatures of tonoplast ATPase and Ppase activities in the WSP were 43℃ and 49℃, respectively and in the chlorenchyma, 37℃ and 46℃, respectively. Th e activities of tonoplast ATPase increased with temperature rise when the tempe ratures were less than their optimum temperatures and decreased when the tempera tures were higher than their optimum temperatures. The increase in tonoplast(Ppa se) ATPase activity in the chlorenchyma was faster than that in the WSP in the t emperature ranges of(10-46℃)10-37℃, and the chlorenchyma obviously had(relativ ely

  8. Utilización de la biomasa residual del cultivo de la piña (Ananas Comosus) para la desproteinización enzimática de desechos de la actividad camaronera

    OpenAIRE

    2013-01-01

    Se realizó un estudio para comprobar la capacidad desproteinizante de extractos proteicos obtenidos a partir de desechos de rastrojo de piña a diferentes concentraciones sobre los sub-productos de la actividad camaronera. El extracto proteolítico de los desechos de rastrojo de piña se obtuvo a partir de desechos de tallos utilizando un buffer de acido sulfúrico. Este extracto se caracterizó al determinar el contenido de proteína, de azucares totales y su actividad enzimática, contra un patrón...

  9. Aplicación y comparación de pretratamientos totalmente libres de cloro en residuos de piña (Ananas comosus y zapote mamey (Pouteria sapota para la obtención de carboximetilcelulosa

    Directory of Open Access Journals (Sweden)

    José Manuel Juárez Barrientos

    2011-01-01

    Full Text Available Derivado del aprovechamiento de la piña y el zapote mamey en la región de Tuxtepec, Oaxaca, México, se generan grandes cantidades de residuos, siendo estos en su mayoría materiales lignocelulósicos compuestos principalmente por celulosa, hemicelulosa y lignina a través de los cuales es posible obtener derivados como la carboximetilcelulosa (CMC aislando previamente la fracción celulósica de los demás componentes. Estos residuos fueron caracterizados previamente en base a un análisis químico proximal, contenido de celulosa, hemicelulosa y lignina para después ser sometidos a algún pretratamiento. Se probaron tres pretratamientos: (A Térmico-Oxidativo, (B Térmico-Alcalino y (C Térmico-Alcalino-Oxidativo, obteniendo con el pretratamiento C residuos con contenidos de celulosa superiores al 90 %. Posteriormente se obtuvo CMC de los residuos derivados de los tres diferentes pretratamientos por el método de eterificación directa, determinando el efecto de los pretratamientos sobre el grado de sustitución, pureza, solubilidad y comportamiento reológico al flujo de las CMCs, logrando obtener CMC únicamente a partir de los residuos de piña y zapote mamey derivados del pretratamiento C, con propiedades similares a una CMC comercial de grado alimenticio.

  10. Aplicación y comparación de pretratamientos totalmente libres de cloro en residuos de piña (Ananas comosus y zapote mamey (Pouteria sapota para la obtención de carboximetilcelulosa

    Directory of Open Access Journals (Sweden)

    José Manuel Juárez Barrientos

    2011-06-01

    Full Text Available Derivado del aprovechamiento de la piña y el zapote mamey en la región de Tuxtepec, Oaxaca, México, se generan grandes cantidades de residuos, siendo estos en su mayoría materiales lignocelulósicos compuestos principalmente por celulosa, hemicelulosa y lignina a través de los cuales es posible obtener derivados como la carboximetilcelulosa (CMC aislando previamente la fracción celulósica de los demás componentes. Estos residuos fueron caracterizados previamente en base a un análisis químico proximal, contenido de celulosa, hemicelulosa y lignina para después ser sometidos a algún pretratamiento. Se probaron tres pretratamientos: (A Térmico-Oxidativo, (B Térmico-Alcalino y (C Térmico-Alcalino-Oxidativo, obteniendo con el pretratamiento C residuos con contenidos de celulosa superiores al 90 %. Posteriormente se obtuvo CMC de los residuos derivados de los tres diferentes pretratamientos por el método de eterificación directa, determinando el efecto de los pretratamientos sobre el grado de sustitución, pureza, solubilidad y comportamiento reológico al flujo de las CMCs, logrando obtener CMC únicamente a partir de los residuos de piña y zapote mamey derivados del pretratamiento C, con propiedades similares a una CMC comercial de grado alimenticio.

  11. Utilización de la biomasa residual del cultivo de la piña (Ananas Comosus para la desproteinización enzimática de desechos de la actividad camaronera

    Directory of Open Access Journals (Sweden)

    Javier Rodrigo Alpízar Cordero

    2013-03-01

    Full Text Available Se realizó un estudio para comprobar la capacidad desproteinizante de extractos proteicos obtenidos a partir de desechos de rastrojo de piña a diferentes concentraciones sobre los sub-productos de la actividad camaronera. El extracto proteolítico de los desechos de rastrojo de piña se obtuvo a partir de desechos de tallos utilizando un buffer de acido sulfúrico. Este extracto se caracterizó al determinar el contenido de proteína, de azucares totales y su actividad enzimática, contra un patrón de bromelina comercial. Los resultados obtenidos indicaron que el uso del extracto crudo de rastrojo de piña en forma directa logró desproteinizar el 97% de los desechos de camarón, el cual resultó más efectivo que cualquier dilución de esta misma, en un tiempo de 24 horas. Los resultados sugieren que los desechos de la comercialización de la piña pueden ser considerados como potenciales bioagentes para la desproteinizacion de desechos de camarón. Con esto se genera una alternativa biotecnológica para sustituir los procesos químicos tradicionales que utilizan hidróxido de sodio. Esta novedosa metodología promoverá nuevas aplicaciones para los desechos del cultivo de la piña y reducirá los costos y la contaminación del proceso de producción de quitina y quitosano.

  12. Meta analysis of pineapple plant (Ananas comosus) as rumniant feed.

    OpenAIRE

    López-Herrera, Michael; WingChing-Jones, Rodolfo; Rojas-Baurillón, Augusto

    2014-01-01

    The objective of this study was to determine the potential use of pineapple by-products in the diet of ruminants. A compilation of data and subsequent statistical analysis was performed on globally published research up until 2013. The results were used to create a nutritional characterization of the pineapple organ by organ, including whole plant, stover (leaves, stem and ratoons), fruit crown, heart, skin and fruit pulp, stem and roots. This characterization aims to optimize its use in rumi...

  13. 78 FR 26540 - Importation of Jackfruit, Pineapple, and Starfruit From Malaysia Into the Continental United States

    Science.gov (United States)

    2013-05-07

    ... (Ananas comosus (L.) Merr.), or starfruit (Averrhoa carambola L.) from Malaysia. The national plant...), Pineapple (Ananas comosus), and Starfruit (Averrhoa carambola) with Stems, from Malaysia into the... (Artocarpus heterophyllus Lam.), pineapple (Ananas comosus (L.) Merr.), and starfruit (Averrhoa carambola...

  14. LC-MS/MS Identification of a Bromelain Peptide Biomarker from Ananas comosus Merr

    Directory of Open Access Journals (Sweden)

    Eric R. Secor

    2012-01-01

    Full Text Available Bromelain (Br is a cysteine peptidase (GenBank AEH26024.1 from pineapple, with over 40 years of clinical use. The constituents mediating its anti-inflammatory activity are not thoroughly characterized and no peptide biomarker exists. Our objective is to characterize Br raw material and identify peptides in the plasma of Br treated mice. After SDS-PAGE in-gel digestion, Br (VN#3507; Middletown, CT, USA peptides were analyzed via LC/MS/MS using 95% protein probability, 95% peptide probability, and a minimum peptide number = 5. Br spiked mouse plasma (1 ug/ul and plasma from i.p. treated mice (12 mg/kg were assessed using SRM. In Br raw material, we identified seven proteins: four proteases, one jacalin-like lectin, and two protease inhibitors. In Br spiked mouse plasma, six proteins (ananain, bromelain inhibitor, cysteine proteinase AN11, FB1035 precursor, FBSB precursor, and jacalin-like lectin were identified. Using LC/MS/MS, we identified the unique peptide, DYGAVNEVK, derived from FB1035, in the plasma of i.p. Br treated mice. The spectral count of this peptide peaked at 6 hrs and was undetectable by 24 hrs. In this study, a novel Br peptide was identified in the plasma of treated mice for the first time. This Br peptide could serve as a biomarker to standardize the therapeutic dose and maximize clinical utility.

  15. Bromelain, the enzyme complex of pineapple (Ananas comosus) and its clinical application. An update.

    Science.gov (United States)

    Taussig, S J; Batkin, S

    1988-01-01

    After a short description of the uses of pineapple as folk medicine by the natives of the tropics, the more important new pharmaceutical applications of bromelain, reported between 1975 and 1978, are presented. Although the exact chemical structure of all active components of bromelain is not fully determined, this substance has shown distinct pharmacological promise. Its properties include: (1) interference with growth of malignant cells; (2) inhibition of platelet aggregation; (3) fibrinolytic activity; (4) anti-inflammatory action; (5) skin debridement properties. These biological functions of bromelain, a non-toxic compound, have therapeutic values in modulating: (a) tumor growth; (b) blood coagulation; (c) inflammatory changes; (d) debridement of third degree burns; (e) enhancement of absorption of drugs. The mechanism of action of bromelain affecting these varied biological effects relates in part to its modulation of the arachidonate cascade.

  16. Management of Rotylenchulus reniformis in pineapple, Ananas comosus, by intercycle cover crops.

    Science.gov (United States)

    Wang, K-H; Sipes, B S; Schmitt, D P

    2002-06-01

    The effects of intercycle cover crops on Rotylenchulus reniformis population densities in pineapple were evaluated in one greenhouse and two field experiments. In the greenhouse, Crotalaria juncea, Brassica napus, and Tagetes erecta were planted for 3 months and then incorporated. These treatments were compared to weedy fallow with or without 1,3-dichloropropene (1,3-D) in three soils (Makawao fallow, Wahiawa fallow, and Wahiawa pineapple) naturally infested with R. reniformis. All cover crop incorporation suppressed R. reniformis numbers in cowpea more than did the weedy treatment in the Makawao (P < 0.05) but not in the Wahiawa soils. Crotalaria juncea treatment increased bacterivorous nematodes and nematode-trapping fungal population densities more than the other treatments in Makawao fallow and Wahiawa pineapple-planted soils. The field trials included the same plants as well as Sinapis alba. Treatments with Crotalaria juncea and 1,3-D maintained lower R. reniformis population densities on pineapple longer than other cover crops or weedy fallow treatments. Crotalaria juncea could have suppressed R. reniformis because it is a poor host and because it enhances nematode-trapping fungi when incorporated into soil. Treatment with 1,3-D reduced microbial activities but produced the greatest pineapple yield.

  17. Effectiveness of radiation processing for elimination of Salmonella Typhimurium from minimally processed pineapple (Ananas comosus Merr.).

    Science.gov (United States)

    Shashidhar, Ravindranath; Dhokane, Varsha S; Hajare, Sachin N; Sharma, Arun; Bandekar, Jayant R

    2007-04-01

    The microbiological quality of market samples of minimally processed (MP) pineapple was examined. The effectiveness of radiation treatment in eliminating Salmonella Typhimurium from laboratory inoculated ready-to-eat pineapple slices was also studied. Microbiological quality of minimally processed pineapple samples from Mumbai market was poor; 8.8% of the samples were positive for Salmonella. D(10) (the radiation dose required to reduce bacterial population by 90%) value for S. Typhimurium inoculated in pineapple was 0.242 kGy. Inoculated pack studies in minimally processed pineapple showed that the treatment with a 2-kGy dose of gamma radiation could eliminate 5 log CFU/g of S. Typhimurium. The pathogen was not detected from radiation-processed samples up to 12 d during storage at 4 and 10 degrees C. The processing of market samples with 1 and 2 kGy was effective in improving the microbiological quality of these products.

  18. Evaluation of antibacterial activities of silver nanoparticles green-synthesized using pineapple leaf (Ananas comosus).

    Science.gov (United States)

    Emeka, Elemike Elias; Ojiefoh, Oseghale Charles; Aleruchi, Chuku; Hassan, Labulo Ayomide; Christiana, Owoseni Mojisola; Rebecca, Mfon; Dare, Enock Olugbenga; Temitope, Adesuji Elijah

    2014-02-01

    Pineapple leaf was used in this study for the synthesis of silver nanoparticles based on the search for sustainable synthetic means. Indeed, this offered an economical and sustainable synthetic route relative to expensive and toxic chemical methods. The leaf extract was used and the corresponding nanoparticles obtained were subjected to UV-vis analysis at different times. The UV-vis was used to monitor the silver nanoparticle formation through sampling at time intervals. The formation of silver nanoparticles was apparently displayed within 2 min with evidence of surface plasmon bands (SPB) between 440 and 460 nm. The crystals was equally characterized using FTIR, X-ray diffraction methods and TEM. The different results obtained suggested the appearance of silver nanoparticles (SNPs) as determined by the process parameters with a particle size of 12.4 nm. The sample was further screened against Staphylococcus aureus, Streptococcus pneumoniae, Proteus mirabilis and Escherichia coli using Gentamicin as control. From the results, there is evidence of inhibition towards bacteria growth. It can now be inferred from the studies that biosynthesis of nanoparticles could be a gateway to our numerous health issues.

  19. In vitro activity of pineapple extracts (Ananas comosus, Bromeliaceae) on Rhipicephalus (Boophilus) microplus (Acari: Ixodidae).

    Science.gov (United States)

    Domingues, Luciana Ferreira; Giglioti, Rodrigo; Feitosa, Karina Alves; Fantatto, Rafaela Regina; Rabelo, Márcio Dias; Oliveira, Márcia Cristina de Sena; Oliveira, Gilson Pereira de; Bechara, Gervasio Henrique; Chagas, Ana Carolina de Souza

    2013-07-01

    Measures to control the cattle tick, Rhipicephalus (Boophilus) microplus, based only on chemical products are becoming unsustainable, mainly because of the development of resistance. The objective of this study was to test the effect of the aqueous extract of pineapple skin (AEPS) and bromelain extracted from the stem (Sigma-Aldrich®, B4882) on engorged females and larvae of R. (B.) microplus in vitro. These substances were diluted in water and evaluated at eight concentrations. Engorged females were collected and distributed in groups of 10, with three repetitions for each treatment. After immersion in the solutions, the females were placed in an incubator for observation of survival, oviposition and larval hatching. The larval packet method was used, also with three repetitions with about 100 larvae each. The packets were incubated and the readings were performed after 24 h. The estimated reproduction and efficacy of the solutions were calculated. The LC(50) and LC(90) were estimated using the Probit procedure of the SAS program. The eight concentrations were compared within each treatment by the Tukey test. For the experiment with engorged females, the most effective concentrations were 125, 250 and 500 mg/mL: 33%, 48% and 59% for the AEPS and 27%, 51% and 55% for the bromelain. The LC(50) and LC(90) values were, respectively, 276 and 8691 mg/mL for AEPS and 373 and 5172 mg/mL for bromelain. None of the dilutions tested was effective against the larvae of R. (B.) microplus. This is the first report of the action of pineapple extracts or their constituents on cattle ticks. The results demonstrate that further studies regarding composition of tick cuticle, with evaluation of other solvents and formulations, should be conducted seeking to enhance the effect of pineapple extracts and compounds against this ectoparasite.

  20. Isolation and characterization of a FLOWERING LOCUS T homolog from pineapple (Ananas comosus (L.) Merr).

    Science.gov (United States)

    Lv, LingLing; Duan, Jun; Xie, JiangHui; Wei, ChangBin; Liu, YuGe; Liu, ShengHui; Sun, GuangMing

    2012-09-01

    FLOWERING LOCUS T (FT)-like genes are crucial regulators of flowering in angiosperms. A homolog of FT, designated as AcFT (GenBank ID: HQ343233), was isolated from pineapple cultivar Comte de Paris by reverse transcriptase polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE). The cDNA sequence of AcFT is 915 bp in length and contains an ORF of 534 bp, which encodes a protein of 177 aa. Molecular weight was 19.9 kDa and isoelectric point was 6.96. The deduced protein sequence of AcFT was 84% and 82% identical to homologs encoded by CgFT in Cymbidium goeringii and OgFT in Oncidium Gower Ramsey respectively. Quantitative real-time PCR (qRT-PCR) analyses showed that the expression of AcFT was high in flesh and none in leaves. qRT-PCR analyses in different stages indicated that the expression of AcFT reached the highest level on 40 d after flower inducing, when the multiple fruit and floral organs were forming. The 35S::AcFT transgenic Arabidopsis plants flowered earlier and had more inflorescences or branches than wild type plants.

  1. Kinetics studies with fruit bromelain (Ananas comosus) in the presence of cysteine and divalent ions.

    Science.gov (United States)

    Kaur, Tajwinder; Kaur, Amandeep; Grewal, Ravneet K

    2015-09-01

    The kinetics of cysteine and divalent ion modulation viz. Ca(2+), Cu(2+), Hg(2+) of fruit bromelain (EC 3.4.22.33) have been investigated in the present study. Kinetic studies revealed that at pH 4.5, cysteine induced V-type activation of bromelain catalyzed gelatin hydrolysis. At pH 3.5, Ca(2+) inhibited the enzyme noncompetitively, whereas, both K-and V-type activations of bromelain were observed in the presence of 0.5 mM Ca(2+) at pH 4.5 and 7.5. Bromelain was inhibited competitively at 0.6 mM Cu(2+) ions at pH 3.5, which changed to an uncompetitive inhibition at pH 4.5 and 7.5. An un-competitive inhibition of bromelain catalyzed gelatin hydrolysis was observed in the presence of 0.6 mM Hg(2+) at pH 3.5 and 4.5. These findings suggest that divalent ions modulation of fruit bromelain is pH dependent.

  2. Study on evaluation of silage from pineapple (Ananas comosus) fruit residue as livestock feed.

    Science.gov (United States)

    Gowda, Nisarani Kollurappa Shivakumar; Vallesha, Naglapura Chandrashekara; Awachat, Vaibhav Bhagvan; Anandan, Samireddypalli; Pal, Din Taran; Prasad, Cadaba Srinivasa

    2015-03-01

    Pineapple is a commercially important fruit crop grown in Asian and African countries. Pineapple fruit residue (PFR) accounts for more than 65% of the processed fruits, and its disposal is a major problem due to its high moisture and sugar content predisposing it to fungal growth and spoilage. Silage technique was adopted to address this problem, and the PFR silage was evaluated for its feeding value. It was observed that on 15th day, the pH of PFR silage was 4.2-4.3 and lactic acid content was 6-8% (DM basis). Combination of 4 parts leafy crown and 1 part peels/pomace was found very ideal to achieve moisture content of 65-70% and produced a good quality silage with minimum fungal count (<3-4 colony forming units) on 15th day of ensiling. Nutritive value in terms of energy and minerals was superior to maize green fodder. Feeding trial in two groups of sheep with 10 numbers in each group fed total mixed ration (TMR) comprising 62% PFR/maize silage and 48% concentrate mixture (DM basis) for 75-day period did not show any adverse effects on nutrient utilization (DM, CP, NDF, ADF), serum biochemical (total protein, creatinine, urea nitrogen, SGOT, SGPT), and mineral profile (Ca, P, Mg, Cu, Zn, Mn) and supported a daily growth rate of 140 g. The overall performance was similar to those sheep fed TMR with maize green fodder silage. Feeding PFR silage replacing hybrid napier green fodder in two groups of cows with eight in each group showed an improvement in average daily milk yield by 3.0 lit per cow and fat content by 0.6 U fed PFR silage-based TMR as compared to cows fed hybrid napier green fodder-based TMR. In both studies (sheep or cows), there was no evidence of metabolic or health-related disorders indicating that PFR silage was effectively utilized. Pineapple fruit residue that was hitherto wasted was successfully converted to silage and was found to be a valuable alternative to conventional green fodder. Ensiling of PFR not only improved the economics of feeding but also helped in overcoming the disposal problem.

  3. Droplet vitrification technique for cryopreservation of different pineapple (Ananas comosus L. Merrill) accessions

    Science.gov (United States)

    Germplasm conservation of pineapple is crucial to secure the genetic variability of the genus for breeding programs and supporting new research. Long-term conservation is done through cryopreservation, by storing cells or tissues at ultra-low temperature in liquid nitrogen (LN; -196°C) or in the LN ...

  4. Cytokinins and auxin communicate nitrogen availability as long-distance signal molecules in pineapple (Ananas comosus).

    Science.gov (United States)

    Tamaki, Vívian; Mercier, Helenice

    2007-11-01

    This work aimed at identifying a possible role of phytohormones in long-distance (root-shoot) signaling under nitrogen deficiency. Three-months old pineapple plants were transferred from Murashige and Skoog (MS) medium to nitrogen-free MS (-N). During the first 24h on -N, 20 plants were harvested every 4h. After 30 days in -N, the remaining plants were transferred back to regular MS (+N) and 20 plants harvested every 4h for the first 24h. Following the harvests, endogenous levels of nitrate (NO(3)(-)), indole-3-acetic acid (IAA), isopentenyladenine (iP), isopentenyladenine riboside (iPR), zeatin (Z) and zeatin riboside (ZR) were analyzed in roots and leaves. In N-starved plants, the NO(3)(-) level dropped by 20% in roots between the first (4h) and the second harvest (8h). In leaves a reduction of 20% was found 4h later. Accumulation of IAA peaked in leaves at 16h. In roots, the accumulation of IAA only started at 16h while the leaf content was already in decline, which suggests that the hormone might have traveled from the leaves to the roots, communicating N-shortage. The contents of the four cytokinins were generally low in both, shoot and roots, and remained almost unchanged during the 24h of analysis. After N re-supply, roots showed a NO(3)(-) peak at 8h whereas the foliar concentration increased 4h later. Hormone levels in roots climaxed at 8h, this coinciding with the highest NO(3)(-) concentration. In leaf tissue, a dramatic accumulation was only observed for Z and ZR, and the peak was seen 4h later than in roots, suggesting that Z-type cytokinins might have traveled from the roots to the leaves. These findings provide evidence that there is a signaling pathway for N availability in pineapple plants, communicated upwards through cytokinins (N-supplemented plants) and downwards through auxin (N-starved plants).

  5. Structural characterization of a glucuronoarabinoxylan from pineapple (Ananas comosus (L.) Merrill) gum exudate.

    Science.gov (United States)

    Simas-Tosin, Fernanda F; de Souza, Lauro M; Wagner, Ricardo; Pereira, Graciele C Z; Barraza, Ruth R; Wendel, Cinthia F; Sassaki, Guilherme L; Iacomini, Marcello; Gorin, Philip A J

    2013-04-15

    Native polysaccharide from pineapple gum (PANP) was obtained following alkaline extraction of gum and fractionation with cetylpyridinium chloride. It was characterized as a glucuronoarabinoxylan using NMR, methylation data, controlled Smith degradation, carboxy-reduction, and ESI-MS of oligosaccharides produced on mild acid hydrolysis of PANP. HSPEC-MALLS-RI of carboxy-reduced fraction showed homogeneous profile (Mw 1.943×10(5) g/mol). PANP was composed of Ara, Xyl, Gal, and GlcpA (40:23:7:30 molar ratio). Its main chain presented (1→4)-linked β-xylan, highly substituted at O-2 and O-3 by side chains of 3-O- and 3,5-di-O-linked α-Araf, 2-O- and 4-O-linked α-GlcpA, and nonreducing end-units of α-Araf, β-Arap, β-Galp, and α-GlcpA. ESI-MS of a mixture of oligosaccharides formed on the mild acid hydrolysis of PANP was consistent with repetitive structures of α-GlcpA O-3 linked at β-Xylp units, whereas in others glucuronoarabinoxylan-type gum exudates, α-GlcpA units had been previously found to be linked at O-2.

  6. Tissue distribution, synthesis stage, and ethylene induction of pineapple (Ananas comosus) chitinases.

    Science.gov (United States)

    Taira, Toki; Toma, Noriko; Ichi, Marika; Takeuchi, Makoto; Ishihara, Masanobu

    2005-04-01

    We examined the tissue distribution, synthesis stage, and ethylene induction of three types of pineapple chitinase using chitinase activity gel and immunoblot analysis. Type A (acidic class III) exists in all tissues, while type B (weakly basic class I, which has strong antifungal activity) and type C (acidic class I) are localized mainly in the leaf and stem. In a pericarp, type A exists at all stages during fruit development, while type B and type C exist only at the early stage. Synthesis of type A is induced by ethylene, while that of types B and C is not affected by it. These results suggest that the physiological roles of these three types of chitinase in pineapple are different.

  7. AISOLASI DAN UJI DAYA ANTIMIKROBA EKSTRAK KULIT NANAS (Ananas comosus L. Merr

    Directory of Open Access Journals (Sweden)

    M H Setiawan

    2017-03-01

    Full Text Available Kandungan senyawa flavonoid dalam limbah kulit nanas memiliki prospek positif sebagai antimikroba. Penelitian ini bertujuan untuk mengetahui efektivitas antimikroba ekstrak kulit nanas basah (KNB dan ekstrak kulit nanas kering (KNK dan mengetahui jenis senyawa flavonoid yang berperan sebagai antimikroba terhadap S. aureus dan E. coli. Flavonoid diisolasi menggunakan metode Domestic Microwave Maceration Extraction (DMME dan partisi. Aktivitas antimikroba diuji menggunakan metode difusi sumuran. Berdasarkan hasil penelitian, ekstrak etil asetat KNB maupun ekstrak etil asetat KNK berpotensi sebagai antimikroba, namun ekstrak etil asetat KNB lebih efektif menghambat bakteri S. aureus dan E. coli dengan diameter daerah hambat (DDH berturut-turut 13 mm dan 15 mm dibanding ekstrak etil asetat KNK dengan nilai DDH berturut-turut 12 dan 14,25 mm. Senyawa flavonoid dalam kulit nanas yang berperan sebagai antimikroba diduga merupakan golongan flavanon (KNB dan dihidroflavonol (KNK.Flavonoid compound in pineapple peel waste have positive prospects for antimicrobial. This study aims at understanding effectiveness of wet pineapple peel extract and dried pineapple peel extract antimicrobial and to know the type of flavonoid compound which act as antimicrobial against S.aureus and E.coli. Flavonoid was isolated using Domestic Microwave Maceration Extraction (DMME and partitions. Antimicrobial activity was tested using pitting diffusion method. Based on the research result, wet pineapple peel extract and dried pineapple peel extract are potential to be antimicrobial. However wet pineapple peel extract is more effective to inhibit bacteria S.aureus and E.coli with Inhibitory Regional Diameter respectively 13 mm and 15 mm compared to dried pineapple peel extract with Inhibitory Regional Diameter respectively 12 and 14,25 mm. Flavonoid compound in pineapple peel that acts as an antimicrobial is thought to be flavanones (wet pineapple peel and dihydroflavonol (dried pineapple peel.

  8. Polyphenol oxidase and peroxidase expression in four pineapple varieties (Ananas comosus L.) after a chilling injury.

    Science.gov (United States)

    Raimbault, Astrid-Kim; Marie-Alphonsine, Paul-Alex; Horry, Jean-Pierre; Francois-Haugrin, Madlyn; Romuald, Karell; Soler, Alain

    2011-01-12

    Pineapple internal browning (IB) is a chilling injury that produces enzymatic browning associated with flesh translucency. Pineapple biodiversity allowed the investigation of how polyphenol oxidase (PPO) and peroxidase (POD) activities with their different isoforms are involved in the IB mechanism. Fruits of four varieties that expressed IB symptoms differently, Smooth Cayenne (SCay) and the hybrids MD2, Flhoran 41 (Flh 41), and Flhoran 53 (Flh 53), were stressed by cold. The susceptible varieties showed classical brown spots but different patterns of IB, whereas MD2 and controls showed no IB. Enzymatic activities were measured on fruit protein extracts and PPO and POD isoforms separated on mini-gels (PhastSystem). Only PPO activity was significantly enhanced in the presence of IB. Up to six PPO isoforms were identified in the susceptible varieties. PPO was barely detectable in the nonsusceptible variety MD2 and in controls. The number of PPO isoforms and the total PPO activity after chilling are varietal characteristics.

  9. Surmounting self-incompatibility in pineapple (Ananas comosus L. ) with pollen irradiation

    Energy Technology Data Exchange (ETDEWEB)

    Subramanian, N.; Iyer, C.P.A.; Singh, R. (Indian Inst. of Horticultural Research, Bangalore)

    Irradiation was investigated as a tool to overcome self-incompatibility in pineapple. Pollen grains were irradiated with 15, 20, 25 kr gamma radiation from cobalt-60 and were used for pollination on the stigma of the same inflorescence. Observations on seed-set were made when the fruits were ripe. It was observed that 20 kr gamma radiation was effective in overcoming self-incompatibility.

  10. ENERGY SOURCES AFFECT IN VITRO PROPAGATION AND SUBSEQUENT ACCLIMATIZATION OF ANANAS COMOSUS, VAR. SMOOTH CAYENNE PLANTS

    Directory of Open Access Journals (Sweden)

    Ayelign Mengesha

    2013-06-01

    Full Text Available Plant tissue culture is an inevitable technique to overcome healthy and limited planting materials problems using suitable energy sources. Different carbohydrates have diverse effect on in vitro growing plantlets in terms of growth performance, acclimatization and cost used for micro-propagation. Hence, this paper reports the effects of sucrose, fructose, glucose, table sugar and starch on pineapple in vitro mass propagation and acclimatization as well as the analysis of energy source required cost per a medium. A complete randomized design was used to compare analytic grade sucrose with other four energy sources at 2 and 3 % (w/v. The results revealed that the energy sources with varied concentration strongly influenced the in vitro growth and subsequent acclimatization of pineapple plantlets. Analytic grade sucrose and table sugar at 3 % performed well for in vitro survival rate (100%, shoot amplification (15.3-16.5 shoots, rooting ability (2.5cm long and 12 roots and acclimatization (95.4-97%. However, fructose and glucose required high importation cost (229.1% and 121.9% over analytic grade sucrose, respectively, and have low growth and acclimatization performance next to starch and energy free medium. Thus, table sugar has found to be a suitable alternative energy source for pineapple mass propagation, which saved about 95-97% cost from that of laboratory grade sucrose.

  11. Cloning and expression analysis of a PISTILLATA homologous gene from pineapple (Ananas comosus L. Merr).

    Science.gov (United States)

    Lv, Ling-Ling; Duan, Jun; Xie, Jiang-Hui; Liu, Yu-Ge; Wei, Chang-Bin; Liu, Sheng-Hui; Zhang, Jian-Xia; Sun, Guang-Ming

    2012-01-01

    PISTILLATA (PI)-like genes are crucial regulators of flowering in angiosperms. A homologue of PI, designated as AcPI (Genbank accession number HQ717796), was isolated from pineapple cultivar Comte de Paris by reverse transcriptase polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE). The cDNA sequence of AcPI is 907 bp in length and contains an open reading frame of 594 bp, which encodes a protein of 197 amino acids. The molecular weight was 2.29 kDa and the isoelectric point was 9.28. The alignment showed that AcPI had a high identity with CsPIC2 (78.6%), AoPI (77.4%), OrcPI (75.7%) and HPI2 (72.4%). Quantitative real-time polymerase chain reaction (qRT-PCR) analyses in different tissues showed that the expression pattern of AcPI was different from the B-class genes in eudicots. AcPI was expressed in all the tissues investigated. The expression level was very low in fruit stems, bracts, leaves and sepals, high in petals and carpels, and moderate in apical meristems, flesh and stamens. The qRT-PCR analyses in different stages indicated that the expression of AcPI reached the highest level at 40 days after flower inducement, when the multiple fruit and floral organs were forming. It proved the important role of AcPI in floral organs and fruit development. The 35S::AcPI transgenic Arabidopsis plants flowered earlier and had more inflorescences or branches than wild type plants.

  12. Insight into biosorption equilibrium, kinetics and thermodynamics of crystal violet onto Ananas comosus (pineapple) leaf powder

    Science.gov (United States)

    Chakraborty, Sagnik; Chowdhury, Shamik; Saha, Papita Das

    2012-06-01

    Biosorption performance of pineapple leaf powder (PLP) for removal of crystal violet (CV) from its aqueous solutions was investigated. To this end, the influence of operational parameters such as pH, biosorbent dose, initial dye concentration and temperature were studied employing a batch experimental setup. The biosorption process followed the Langmuir isotherm model with high correlation coefficients ( R 2 > 0.99) at different temperatures. The maximum monolayer biosorption capacity was found to be 78.22 mg g-1 at 293 K. The kinetic data conformed to the pseudo-second-order kinetic model. The activation energy of the system was calculated as 58.96 kJ mol- 1 , indicating chemisorption nature of the ongoing biosorption process. A thermodynamic study showed spontaneous and exothermic nature of the biosorption process. Owing to its low cost and high dye uptake capacity, PLP has potential for application as biosorbent for removal of CV from aqueous solutions.

  13. Relationships between some Thai cultivars of pineapple (Ananas comosus revealed by RAPD analysis

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    Siam Popluechai

    2007-11-01

    Full Text Available RAPD analysis of nine Thai pineapple cultivars, including 'Phulae', 'Sawee', 'Tradsithong', 'Phuket', 'Pattavia', 'Intrachitdang', 'Intrachitkhow', 'Petburi No.1', and 'Nanglae', showed that, of 40 arbitrary 10- mer primers, 17 primers gave 206 DNA fragments ranging from 510 to 4,700 bp. One hundred and forty-five (70.4% of the amplified fragments were polymorphic. RAPD analysis using NTSYS-pc Version 2.01e also showed that the similarity coefficients among the cultivars were 0.643-0. 963. The dendrogram indicated that the cultivars were clustered into 3 groups, consistent with the morphological data. The first group, consisting of 'Phuket', 'Phulae', 'Tradsithong', 'Sawee', and 'Petburi No.1', had morphological characteristics of the Queen group, while those of the second ('Intrachitdang' and 'Intrachitkow' and the third ('Nanglae' and 'Pattavia' groups could be determined morphologically to be members of the Spanish and Cayenne groups, respectively. 'Intrachitdang' and 'Intrachitkow' have similarity coefficient of 0.963, while that of 'Phulae' and 'Phuket' is 0.950. These pairs of cultivars are probably the same cultivars. The morphological differences between them are probably caused by mutations, differences in environment and agricultural practices, or combinations of these factors.

  14. LC-MS/MS Identification of a Bromelain Peptide Biomarker from Ananas comosus Merr.

    Science.gov (United States)

    Secor, Eric R; Szczepanek, Steven M; Singh, Anurag; Guernsey, Linda; Natarajan, Prabitha; Rezaul, Karim; Han, David K; Thrall, Roger S; Silbart, Lawrence K

    2012-01-01

    Bromelain (Br) is a cysteine peptidase (GenBank AEH26024.1) from pineapple, with over 40 years of clinical use. The constituents mediating its anti-inflammatory activity are not thoroughly characterized and no peptide biomarker exists. Our objective is to characterize Br raw material and identify peptides in the plasma of Br treated mice. After SDS-PAGE in-gel digestion, Br (VN#3507; Middletown, CT, USA) peptides were analyzed via LC/MS/MS using 95% protein probability, 95% peptide probability, and a minimum peptide number = 5. Br spiked mouse plasma (1 ug/ul) and plasma from i.p. treated mice (12 mg/kg) were assessed using SRM. In Br raw material, we identified seven proteins: four proteases, one jacalin-like lectin, and two protease inhibitors. In Br spiked mouse plasma, six proteins (ananain, bromelain inhibitor, cysteine proteinase AN11, FB1035 precursor, FBSB precursor, and jacalin-like lectin) were identified. Using LC/MS/MS, we identified the unique peptide, DYGAVNEVK, derived from FB1035, in the plasma of i.p. Br treated mice. The spectral count of this peptide peaked at 6 hrs and was undetectable by 24 hrs. In this study, a novel Br peptide was identified in the plasma of treated mice for the first time. This Br peptide could serve as a biomarker to standardize the therapeutic dose and maximize clinical utility.

  15. Solution structure of a phytocystatin from Ananas comosus and its molecular interaction with papain.

    Science.gov (United States)

    Irene, Deli; Chung, Tse-Yu; Chen, Bo-Jiun; Liu, Ting-Hang; Li, Feng-Yin; Tzen, Jason T C; Wang, Cheng-I; Chyan, Chia-Lin

    2012-01-01

    The structure of a recombinant pineapple cystatin (AcCYS) was determined by NMR with the RMSD of backbone and heavy atoms of twenty lowest energy structures of 0.56 and 1.11 Å, respectively. It reveals an unstructured N-terminal extension and a compact inhibitory domain comprising a four-stranded antiparallel β-sheet wrapped around a central α-helix. The three structural motifs (G(45), Q(89)XVXG, and W(120)) putatively responsible for the interaction with papain-like proteases are located in one side of AcCYS. Significant chemical shift perturbations in two loop regions, residues 45 to 48 (GIYD) and residues 89 to 91 (QVV), of AcCYS strongly suggest their involvement in the binding to papain, consistent with studies on other members of the cystatin family. However, the highly conserved W120 appears not to be involved in the binding with papain as no chemical shift perturbation was observed. Chemical shift index analysis further indicates that the length of the α-helix is shortened upon association with papain. Collectively, our data suggest that AcCYS undergoes local secondary structural rearrangements when papain is brought into close contact. A molecular model of AcCYS/papain complex is proposed to illustrate the interaction between AcCYS and papain, indicating a complete blockade of the catalytic triad by AcCYS.

  16. Odor-active constituents in fresh pineapple (Ananas comosus [L.] Merr.) by quantitative and sensory evaluation.

    Science.gov (United States)

    Tokitomo, Yukiko; Steinhaus, Martin; Büttner, Andrea; Schieberle, Peter

    2005-07-01

    By application of aroma extract dilution analysis (AEDA) to an aroma distillate prepared from fresh pineapple using solvent-assisted flavor evaporation (SAFE), 29 odor-active compounds were detected in the flavor dilution (FD) factor range of 2 to 4,096. Quantitative measurements performed by stable isotope dilution assays (SIDA) and a calculation of odor activity values (OAVs) of 12 selected odorants revealed the following compounds as key odorants in fresh pineapple flavor: 4-hydroxy-2,5-dimethyl-3(2H)-furanone (HDF; sweet, pineapple-like, caramel-like), ethyl 2-methylpropanoate (fruity), ethyl 2-methylbutanoate (fruity) followed by methyl 2-methylbutanoate (fruity, apple-like) and 1-(E,Z)-3,5-undecatriene (fresh, pineapple-like). A mixture of these 12 odorants in concentrations equal to those in the fresh pineapple resulted in an odor profile similar to that of the fresh juice. Furthermore, the results of omission tests using the model mixture showed that HDF and ethyl 2-methylbutanoate are character impact odorants in fresh pineapple.

  17. Enzymatic browning and biochemical alterations in black spots of pineapple [Ananas comosus (L.) Merr.].

    Science.gov (United States)

    Avallone, Sylvie; Guiraud, Joseph-Pierre; Brillouet, Jean-Marc; Teisson, Claude

    2003-08-01

    Penicillium funiculosum Thom. was consistently isolated from pineapple-infected fruitlet (black spots). Polyphenol oxidase, peroxidase, and laccase activities were determined in extracts from contiguous and infected fruitlets. Healthy fruitlets showed a rather high level of polyphenol oxidase (optimum pH 7.0), and this activity was tremendously increased (X 10) in contiguous infected fruitlets. Furthermore, infected fruitlets also exhibited laccase activity (optimum pH 4.0), while peroxidase was rather constant in both fruitlets. Browning reactions were attributed to qualitative and quantitative modifications of the enzymatic equipment (polyphenol oxidase and laccase) (p < 0.0001). In infected fruiltets, sucrose and L-malic acid were present at significantly lower amounts than in healthy ones, likely owing to fungal metabolism (p < 0.0001), whereas cell wall material was three times higher, which could be viewed as a defense mechanism to limit expansion of the mycelium.

  18. La agricultura de las Antillas: un aporte substancial al mundo

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    Mois\\u00E9s Blanco

    2002-01-01

    Full Text Available A partir del 12 de octubre de 1492, el mundo ya no fue el mismo. El almirante Cristóbal Colón tocaba tierras de un nuevo continente, al que posteriormente llamarían América. Cuando descubrió Las Antillas, encontró que estas islas estaban habitadas por diversas tribus donde la agricultura era el epicentro de sus vidas y de sus costumbres. De ella se han heredado muchas, las cuales se encuentran vigentes en el uso cotidiano. El presente trabajo hace descripción de algunas de estas vigencias y se citan ejemplos de su variada influencia como los nombres de: maní (Arachis hypogea L., maíz (Zea mays L., guanábana (Annona muricata L., pitahaya (Hylocerus undatus B & G y tabaco (Nicotiana tabacum L., en usos particulares en el caso del mamey (Mammea americana L., icaco (Chysobalanus icaco L. y yuca (Manihot esculenta C., con propósito alimenticio el quequisque (Xanthosoma sagittifolium Sh, batata (Ipomoea batatas L. y topee tambo (Callathea allonia y de origen autóctono como la piña (Ananas comosus L., nancite (Byrsonima crassifolia H. B. K., guayaba (Psidium guajava L., coco (Cocos nucí- fera L., caimito (Chysophylum cainito L., guayacán (Guayacum sanctum L. y la caoba (Swietenia microphyla L..

  19. Study on the Technology of Compound Beverage Made from Ananas and Pitaya%菠萝火龙果复合饮料制作工艺的研究

    Institute of Scientific and Technical Information of China (English)

    王俊伟; 吕美

    2015-01-01

    A study was conducted on the production technology of a compound beverage made from Ananas(Ananas comosus (Linn.) Merr) and pitaya (Hylocereus undulates Britt) as the raw materials. The effects of stabilizers on the stability and sensory indexes of compound beverage were investigated. The optimum formula of the drink was Ananas∶pitaya=3∶2, juice mixture 40%(v/v), sugar 10%, citric acid 0.15%, with 0.2% (CMC-Na∶xanthan gum=1∶1) as a complex stabilizer. The beverage is featured by light yellow color, uniform structure, aromas of Ananas and pitaya, and pleasant sweetness and sourness.%以新鲜的云南香水菠萝(Ananas comosus(Linn.) Merr)和海南火龙果(Hylocereus undulates Britt)为原料,通过正交试验确定了菠萝火龙果复合饮料的制作工艺参数,并初步分析了稳定剂对复合果汁稳定性以及感官指标的影响。通过实验得出,菠萝火龙果复合饮料的最佳工艺配方为:菠萝汁∶火龙果汁比例为3∶2、复合果汁含量40%(v/v)、白砂糖10%、柠檬酸0.15%、稳定剂(CMC-Na∶黄原胶=1∶1)添加量0.2%。该饮料呈金黄色,质地均匀,具有菠萝和火龙果香气,酸甜适口。

  20. Tarımsal atıklardan elde edilen sürdürülebilir tekstil lifleri: Ananas yaprağı lifleri

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    Ece Kalaycı

    2016-08-01

    Full Text Available Ananas yaprağı lifleri, adından da anlaşılacağı gibi ananas bitkisinin yapraklarından elde edilen doğal bir lif türüdür. Ananas bitkisi genellikle meyvesi için yetiştirildiğinden, her yıl tonlarca ananas yaprağı tarımsal atık olarak ortaya çıkmaktadır. Yapılarındaki selüloz oranı oldukça yüksek olan bu lifler, üstün mekanik özellikleri ve suyu seven yapısı ile iyi bir tekstil malzemesi olarak kabul edilmektedir. Ayrıca, kolay ulaşılabilir, biyobozunur ve geri dönüştürülebilir olması sayesinde özellikle biyo-kompozitlerin üretiminde tercih edilen bir malzeme haline gelmiştir. Hem tarımsal atıkların değerlendirilmesi hem de çevre dostu özellikleri ile sürdürülebilir üretimin hedeflendiği şu günlerde, ananas yaprağı lifi önemli bir hammadde kaynağıdır. Bu derleme çalışmasında, ananas yaprağı liflerinin yapısı, özellikleri, elde ediliş yöntemleri ve kullanım alanları gibi başlıklar detaylı olarak incelenmiştir.

  1. Discovery of precursor and mature microRNAs and their putative gene targets using high-throughput sequencing in pineapple (Ananas comosus var. comosus).

    Science.gov (United States)

    Yusuf, Noor Hydayaty Md; Ong, Wen Dee; Redwan, Raimi Mohamed; Latip, Mariam Abd; Kumar, S Vijay

    2015-10-15

    MicroRNAs (miRNAs) are a class of small, endogenous non-coding RNAs that negatively regulate gene expression, resulting in the silencing of target mRNA transcripts through mRNA cleavage or translational inhibition. MiRNAs play significant roles in various biological and physiological processes in plants. However, the miRNA-mediated gene regulatory network in pineapple, the model tropical non-climacteric fruit, remains largely unexplored. Here, we report a complete list of pineapple mature miRNAs obtained from high-throughput small RNA sequencing and precursor miRNAs (pre-miRNAs) obtained from ESTs. Two small RNA libraries were constructed from pineapple fruits and leaves, respectively, using Illumina's Solexa technology. Sequence similarity analysis using miRBase revealed 579,179 reads homologous to 153 miRNAs from 41 miRNA families. In addition, a pineapple fruit transcriptome library consisting of approximately 30,000 EST contigs constructed using Solexa sequencing was used for the discovery of pre-miRNAs. In all, four pre-miRNAs were identified (MIR156, MIR399, MIR444 and MIR2673). Furthermore, the same pineapple transcriptome was used to dissect the function of the miRNAs in pineapple by predicting their putative targets in conjunction with their regulatory networks. In total, 23 metabolic pathways were found to be regulated by miRNAs in pineapple. The use of high-throughput sequencing in pineapples to unveil the presence of miRNAs and their regulatory pathways provides insight into the repertoire of miRNA regulation used exclusively in this non-climacteric model plant.

  2. The effect of pH on bromelain partition from Ananas comosus by PEG4000/phosphate ATPS

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    Juliana Ferrari Ferreira

    2011-02-01

    Full Text Available In this work, the thermodynamic equilibrium and applying of PEG4000/Phosphate ATPS on the purification of bromelain extracted from pineapple was studied. A rigorous study of the equilibrium curves and tie-line length from PEG4000/phosphate ATPS were done for the pH 6-11 at 25ºC. Results showed that there was augment in the PEG and salt contents with the high pH value from PEG4000/Phosphate ATPS and two-phase formation needed only increasing the PEG content. Two tie-line length at pH 11 from PEG4000/Phosphate ATPS were optimal condition for bromelain purification, one on composition of 14% PEG and 13% salt and other at 12.6% PEG and 12.2% salt, while a 25-62 folds of enzyme was found. SDS-PAGE electrophoreses had one band only, which showed that bromelain was purified. Optimum conditions of bromelain use were found at pH 7 and between 30-40ºC.

  3. Expanded bed adsorption of bromelain (E.C. 3.4.22.33 from Ananas comosus crude extract

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    E. Silveira

    2009-03-01

    Full Text Available This work focuses on the adsorption of Bromelain in expanded bed conditions, such as the adsorption kinetics parameters. The adsorption kinetics parameters showed that after 40 minutes equilibrium was achieved and maximum adsorption capacity was 6.11 U per resin mL. However, the maximum adsorption capacity was only determined by measuring the adsorption isotherm. Only by the Langmuir model the maximum adsorption capacity, Qm, and dissociation constant, kd, values could be estimated as 9.18 U/mL and 0.591, respectively, at 25 ºC and 0.1 mol/L phosphate buffer pH 7.5. A column made of glass with an inner diameter of 1 cm was used for the expanded bed adsorption (EBA. The residence time was reduced 10 fold by increasing the expansion degree 2.5 times; nonetheless, the plate number (N value was reduced only 2 fold. After adsorption, the bromelain was eluted in packed bed mode, with a downward flow. The purification factor was about 13 fold and the total protein was reduced 4 fold. EBA showed to be feasible for purification of bromelain.

  4. Effects of Nitrogen Supplementation on Yeast (Candida utilis Biomass Production by Using Pineapple (Ananas comosus Waste Extracted Medium

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    Rosma, A.

    2007-01-01

    Full Text Available Pineapple waste medium was used to cultivate yeast, Candida utilis. It served as the sole carbon and energy source for the yeast growth. However, pineapple waste media contain very little nitrogen (0.003-0.015% w/v. Various nitrogen sources were incorporate and their effects on biomass, yield and productivity were studied. Significant (p<0.05 increment on biomass production was observed when nitrogen supplement (commercial yeast extract, peptone, ammonium dihydrogen phosphate, ammonium sulphate and potassium nitrate was added into fermentation medium. Commercial yeast extract, Maxarome® which increased 55.2% of biomass production at 0.09% (w/v nitrogen content, is the most suitable among the selected organic source. On the other hand, ammonium dihydrogen phosphate at 0.09% (w/v nitrogen content is comparable inorganic source which enhanced 53.7% of production. Total nitrogen content of each treatment at 0.05% (w/v showed that nitrogen supplied was not fully utilized as substrate limitation in the fermentation medium.

  5. Ensete ventricosum (WELW. CHEESMAN: A CHEAP AND ALTERNATIVE GELLING AGENT FOR PINEAPPLE (Ananas comosus VAR. SMOOTH CAYENNE IN VITRO PROPAGATION

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    Biruk Ayenew

    2012-10-01

    Full Text Available Different mechanisms are tried so far to reduce the production cost of plant tissue culture through increasing multiplication rate and/or substituting expensive components for wider usage of the technology. Subsequently, replacing the most common gelling agent, agar, took an attention since higher proportion of media cost comes from it. Up to now different alternatives were tried though they have drawbacks due to their inherent chemical characteristics. Therefore the search for new alternatives like Enset ventricosum is important. Enset flour, ‘Bulla’, has been tried in this study for the first time to substitute agar and showed no significant difference for shoots number, root number, shoot height, leaf number and an associated fresh weight of the plantlets besides good gelling ability than Agar. Subsequently, ‘Bulla’ at 80g L-1 gelled well and gave 11.8 shoots with 0.95g and 13.33 roots having 1.37 cm length. This rate can also save up to 76 % cost of gelling though significant difference was found for root length of 3.23 cm with Agar, 8 g L-1. This indicates further study on biochemical and/ or hormonal activity and across crop genotypes to use ‘Bulla’ as a cheap alternative commercial gelling agent.

  6. Influence of weight and type of planting material on fruit quality and its heterogeneity in pineapple [Ananas comosus (L.) Merrill].

    Science.gov (United States)

    Fassinou Hotegni, V Nicodème; Lommen, Willemien J M; Agbossou, Euloge K; Struik, Paul C

    2014-01-01

    Cultural practices can affect the quality of pineapple fruits and its variation. The objectives of this study were to investigate (a) effects of weight class and type of planting material on fruit quality, heterogeneity in quality and proportion and yield of fruits meeting European export standards, and (b) the improvement in quality, proportion and yield of fruits meeting export standards when flowering was induced at optimum time. Experiments were conducted in Benin with cvs Sugarloaf (a Perola type) and Smooth Cayenne. In cv. Sugarloaf, experimental factors were weight class of planting material (light, mixed, heavy) and time of flowering induction (farmers', optimum) (Experiment 1). In cv. Smooth Cayenne an additional experimental factor was the type of planting material (hapas, ground suckers, a mixture of the two) (Experiment 2). Fruits from heavy planting material had higher infructescence and fruit weights, longer infructescences, shorter crowns, and smaller crown: infructescence length than fruits from light planting material. The type of planting material in Experiment 2 did not significantly affect fruit quality except crown length: fruits from hapas had shorter crowns than those from ground suckers. Crops from heavy planting material had a higher proportion and yield of fruits meeting export standards than those from other weight classes in Experiment 1 only; also the type of planting material in Experiment 2 did not affect these variates. Heterogeneity in fruit quality was usually not reduced by selecting only light or heavy planting material instead of mixing weights; incidentally the coefficient of variation was significantly reduced in fruits from heavy slips only. Heterogeneity was also not reduced by not mixing hapas and ground suckers. Flowering induction at optimum time increased the proportion and yield of fruits meeting export standards in fruits from light and mixed slip weights and in those from the mixture of heavy hapas plus ground suckers.

  7. Influence of modified atmosphere packaging on volatile compounds and physicochemical and antioxidant attributes of fresh-cut pineapple (Ananas comosus).

    Science.gov (United States)

    Montero-Calderón, Marta; Rojas-Graü, María Alejandra; Aguiló-Aguayo, Ingrid; Soliva-Fortuny, Robert; Martín-Belloso, Olga

    2010-04-28

    The effects of modified atmosphere packaging on volatile compound content and physicochemical and antioxidant attributes of Gold cultivar fresh-cut pineapples were assessed throughout storage at 5 degrees C. Fresh-cut pineapple pieces were packed under LO (low oxygen, 12% O(2), 1% CO(2)), AIR (20.9% O(2)) and HO (high oxygen, 38% O(2)) headspace atmospheres. Methyl butanoate, methyl 2-methylbutanoate, and methyl hexanoate were the most abundant volatiles regardless of the packaging atmosphere and days of storage; whereas most odor active volatiles were methyl and ethyl 2-methylbutanoate, 2,5-dimethyl-4-methoxy-3(2H)-furanone and ethyl hexanoate. Physicochemical attributes of pineapple did not significantly vary, whereas vitamin C content and total antioxidant capacity were lower for fresh-cut pineapple in HO (488 +/- 38 mg/100 mg(fw) and 54.4 +/- 5.7%, respectively) than for LO and AIR packages. Storage life of fresh-cut pineapple was limited to 14 days by volatile compounds losses and fermentation processes.

  8. Influence of weight and type of planting material on fruit quality and its heterogeneity in pineapple [Ananas comosus (L.) Merrill

    NARCIS (Netherlands)

    Fassinou Hotegni, V.N.; Lommen, W.J.M.; Agbossou, E.K.; Struik, P.C.

    2015-01-01

    Cultural practices can affect the quality of pineapple fruits and its variation. The objectives of this study were to investigate (a) effects of weight class and type of planting material on fruit quality, heterogeneity in quality and proportion and yield of fruits meeting European export standards,

  9. Molecular cloning and characterization of four genes encoding ethylene receptors associated with pineapple (Ananas comosus L. flowering

    Directory of Open Access Journals (Sweden)

    Yunhe eLi

    2016-05-01

    Full Text Available Exogenous ethylene, or ethephon, has been widely used to induce pineapple flowering, but the molecular mechanism behind ethephon induction is still unclear. In this study, we cloned four genes encoding ethylene receptors (designated AcERS1a, AcERS1b, AcETR2a and AcETR2b. The 5′ flanking sequences of these four genes were also cloned by self-formed adaptor PCR and SiteFinding-PCR, and a group of putative cis-acting elements was identified. Phylogenetic tree analysis indicated that AcERS1a, AcERS1b, AcETR2a and AcETR2b belonged to the plant ERS1s and ETR2/EIN4-like groups. Quantitative real-time PCR showed that AcETR2a and AcETR2b (subfamily 2 were more sensitive to ethylene treatment compared with AcERS1a and AcERS1b (subfamily 1. The relative expression of AcERS1b, AcETR2a and AcETR2b was significantly increased during the earlier period of pineapple inflorescence formation, especially at 1-9 days after ethylene treatment (DAET, whereas AcERS1a expression changed less than these three genes. In situ hybridization results showed that bract primordia (BP and flower primordia (FP appeared at 9 and 21 DAET, respectively, and flowers were formed at 37 DAET. AcERS1a, AcERS1b, AcETR2a and AcETR2b were mainly expressed in the shoot apex at 1-4 DAET; thereafter, with the appearance of BP and FP, higher expression of these genes was found in these new structures. Finally, at 37 DAET, the expression of these genes was mainly focused in the flower but was also low in other structures. These findings indicate that these four ethylene receptor genes, especially AcERS1b, AcETR2a and AcETR2b, play important roles during pineapple flowering induced by exogenous ethephon.

  10. Ananas comosus L. Leaf Phenols and p-Coumaric Acid Regulate Liver Fat Metabolism by Upregulating CPT-1 Expression

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    Weidong Xie

    2014-01-01

    Full Text Available In this study, we aimed to investigate the effect and action mechanisms of pineapple leaf phenols (PLPs on liver fat metabolism in high-fat diet-fed mice. Results show that PLP significantly reduced abdominal fat and liver lipid accumulation in high-fat diet-fed mice. The effects of PLP were comparable with those of FB. Furthermore, at the protein level, PLP upregulated the expression of carnitine palmitoyltransferase 1 (CPT-1, whereas FB had no effects on CPT-1 compared with the HFD controls. Regarding mRNA expression, PLP mainly promoted the expression of CPT-1, PGC1a, UCP-1, and AMPK in the mitochondria, whereas FB mostly enhanced the expression of Ech1, Acox1, Acaa1, and Ehhadh in peroxisomes. PLP seemed to enhance fat metabolism in the mitochondria, whereas FB mainly exerted the effect in peroxisomes. In addition, p-coumaric acid (CA, one of the main components from PLP, significantly inhibited fat accumulation in oleic acid-induced HepG2 cells. CA also significantly upregulated CPT-1 mRNA and protein expressions in HepG2 cells. We, firstly, found that PLP enhanced liver fat metabolism by upregulating CPT-1 expression in the mitochondria and might be promising in treatment of fatty liver diseases as alternative natural products. CA may be one of the active components of PLP.

  11. Pineapple (Ananas comosus L. Merr.) wine production in Angola: Characterisation of volatile aroma compounds and yeast native flora.

    Science.gov (United States)

    Dellacassa, Eduardo; Trenchs, Oriol; Fariña, Laura; Debernardis, Florencia; Perez, Gabriel; Boido, Eduardo; Carrau, Francisco

    2017-01-16

    A pineapple vinification process was conducted through inoculated and spontaneous fermentation to develop a process suitable for a quality beverage during two successive vintages in Huambo, Angola. Wines obtained with the conventional Saccharomyces cerevisiae strain, were analysed by gas chromatography, and a total of 61 volatile constituents were detected in the volatile fraction and 18 as glycosidically bound aroma compounds. Concentration levels of carbonyl and sulphur compounds were in agreement with the limited information reported about pineapple fruits of other regions. We report, for the first time in pineapple wines, the presence of significant concentrations of lactones, ketones, terpenes, norisoprenoids and a variety of volatile phenols. Eight native yeast strains were isolated from spontaneous batches. Further single-strain fermentations allowed us to characterise their suitability for commercial fermentation. Three native strains (Hanseniaspora opuntiae, H. uvarum and Meyerozyma guilliermondii) were selected with sensory potential to ferment pineapple fruits with increased flavour diversity. Results obtained here contribute to a better understanding of quality fermentation alternatives of this tropical fruit in subtropical regions.

  12. Silencing of the ACC synthase gene ACACS2 causes delayed flowering in pineapple [Ananas comosus (L.) Merr.].

    Science.gov (United States)

    Trusov, Yuri; Botella, José Ramón

    2006-01-01

    Flowering is a crucial developmental stage in the plant life cycle. A number of different factors, from environmental to chemical, can trigger flowering. In pineapple, and other bromeliads, it has been proposed that flowering is triggered by a small burst of ethylene production in the meristem in response to environmental cues. A 1-amino-cyclopropane-1-carboxylate synthase (ACC synthase) gene has been cloned from pineapple (ACACS2), which is induced in the meristem under the same environmental conditions that induce flowering. Two transgenic pineapple lines have been produced containing co-suppression constructs designed to down-regulate the expression of the ACACS2 gene. Northern analysis revealed that the ACACS2 gene was silenced in a number of transgenic plants in both lines. Southern hybridization revealed clear differences in the methylation status of silenced versus non-silenced plants by the inability of a methylation-sensitive enzyme to digest within the ACACS2 DNA extracted from silenced plants, indicating that methylation is the cause of the observed co-suppression of the ACACS2 gene. Flowering characteristics of the transgenic plants were studied under field conditions in South East Queensland, Australia. Flowering dynamics studies revealed significant differences in flowering behaviour, with transgenic plants exhibiting silencing showing a marked delay in flowering when compared with non-silenced transgenic plants and control non-transformed plants. It is argued that the ACACS2 gene is one of the key contributors towards triggering 'natural flowering' in mature pineapples under commercial field conditions.

  13. Molecular Cloning and Characterization of Four Genes Encoding Ethylene Receptors Associated with Pineapple (Ananas comosus L.) Flowering.

    Science.gov (United States)

    Li, Yun-He; Wu, Qing-Song; Huang, Xia; Liu, Sheng-Hui; Zhang, Hong-Na; Zhang, Zhi; Sun, Guang-Ming

    2016-01-01

    Exogenous ethylene, or ethephon, has been widely used to induce pineapple flowering, but the molecular mechanism behind ethephon induction is still unclear. In this study, we cloned four genes encoding ethylene receptors (designated AcERS1a, AcERS1b, AcETR2a, and AcETR2b). The 5' flanking sequences of these four genes were also cloned by self-formed adaptor PCR and SiteFinding-PCR, and a group of putative cis-acting elements was identified. Phylogenetic tree analysis indicated that AcERS1a, AcERS1b, AcETR2a, and AcETR2b belonged to the plant ERS1s and ETR2/EIN4-like groups. Quantitative real-time PCR showed that AcETR2a and AcETR2b (subfamily 2) were more sensitive to ethylene treatment compared with AcERS1a and AcERS1b (subfamily 1). The relative expression of AcERS1b, AcETR2a, and AcETR2b was significantly increased during the earlier period of pineapple inflorescence formation, especially at 1-9 days after ethylene treatment (DAET), whereas AcERS1a expression changed less than these three genes. In situ hybridization results showed that bract primordia (BP) and flower primordia (FP) appeared at 9 and 21 DAET, respectively, and flowers were formed at 37 DAET. AcERS1a, AcERS1b, AcETR2a, and AcETR2b were mainly expressed in the shoot apex at 1-4 DAET; thereafter, with the appearance of BP and FP, higher expression of these genes was found in these new structures. Finally, at 37 DAET, the expression of these genes was mainly focused in the flower but was also low in other structures. These findings indicate that these four ethylene receptor genes, especially AcERS1b, AcETR2a, and AcETR2b, play important roles during pineapple flowering induced by exogenous ethephon.

  14. 菠萝叶酚类成分研究%Studies on phenolic constituents from leaves of pineapple (Ananas comosus)

    Institute of Scientific and Technical Information of China (English)

    王伟; 丁怡; 邢东明; 王金萍; 杜力军

    2006-01-01

    目的:研究菠萝叶中的化学成分.方法:采用硅胶、Sephadex LH-20及ODS柱色谱分离,用波谱学方法确定结构.结果:从乙醇提取物的醋酸乙酯萃取部分分得7个化合物,其结构鉴定为菠萝酰酯(1),1-O-咖啡酰单甘油酯(2),1-O-对羟基肉桂酰单甘油酯(3),咖啡酸(4),对羟基肉桂酸(5),β-谷甾醇(6),β-谷甾醇-3-O-β-D-葡萄糖苷(7).结论:化合物1为新化合物,其余化合物均为首次从该植物中分离得到.

  15. Ananas comosus L. Leaf Phenols and p-Coumaric Acid Regulate Liver Fat Metabolism by Upregulating CPT-1 Expression.

    Science.gov (United States)

    Xie, Weidong; Zhang, Shaobo; Lei, Fan; Ouyang, Xiaoxi; Du, Lijun

    2014-01-01

    In this study, we aimed to investigate the effect and action mechanisms of pineapple leaf phenols (PLPs) on liver fat metabolism in high-fat diet-fed mice. Results show that PLP significantly reduced abdominal fat and liver lipid accumulation in high-fat diet-fed mice. The effects of PLP were comparable with those of FB. Furthermore, at the protein level, PLP upregulated the expression of carnitine palmitoyltransferase 1 (CPT-1), whereas FB had no effects on CPT-1 compared with the HFD controls. Regarding mRNA expression, PLP mainly promoted the expression of CPT-1, PGC1a, UCP-1, and AMPK in the mitochondria, whereas FB mostly enhanced the expression of Ech1, Acox1, Acaa1, and Ehhadh in peroxisomes. PLP seemed to enhance fat metabolism in the mitochondria, whereas FB mainly exerted the effect in peroxisomes. In addition, p-coumaric acid (CA), one of the main components from PLP, significantly inhibited fat accumulation in oleic acid-induced HepG2 cells. CA also significantly upregulated CPT-1 mRNA and protein expressions in HepG2 cells. We, firstly, found that PLP enhanced liver fat metabolism by upregulating CPT-1 expression in the mitochondria and might be promising in treatment of fatty liver diseases as alternative natural products. CA may be one of the active components of PLP.

  16. Pineapple (Ananas comosus cv. pérola ex vitro growth and mycorrhizal colonization affected by in vitro sucrose concentration

    Directory of Open Access Journals (Sweden)

    Alceu Kunze

    2014-09-01

    Full Text Available The aim of this study was to evaluate the effect of sucrose concentration in the culture medium on growth and on the establishment of mycorrhizas during the acclimatization of pineapple cv. Pérola. The plantlets were micropropagated in MS culture medium with 0, 10, 20 and 30 g L-1 of sucrose and then they were acclimatized during 12 weeks under greenhouse conditions, in a sandy soil - compost mixture, uninoculated or inoculated with a Rhizophagus clarus isolate. Plantlets from the culture medium with 20 g and 30 g of sucrose L-1 showed higher shoot and root biomass than those from sugar-free medium. Mycorrhizal colonization was lower in plantlets micropropagated in sucrose-free medium, but the intensity of arbuscules did not differ among treatments. In the 12-week period of acclimatization, mycorrhizal colonization had no effect on plant biomass.

  17. Biology and trapping of stable flies (Diptera: Muscidae) developing in pineapple residues (Ananas comosus) in Costa Rica.

    Science.gov (United States)

    Solórzano, José-Arturo; Gilles, Jeremie; Bravo, Oscar; Vargas, Cristina; Gomez-Bonilla, Yannery; Bingham, Georgina V; Taylor, David B

    2015-01-01

    Pineapple production in Costa Rica increased nearly 300-fold during the last 30 yr, and >40,000 hectares of land are currently dedicated to this crop. At the end of the pineapple cropping cycle, plants are chopped and residues incorporated into the soil in preparation for replanting. Associated with increased pineapple production has been a large increase in stable fly, Stomoxys calcitrans (L.), populations. Stable flies are attracted to, and oviposit in, the decomposing, chopped pineapple residues. In conjunction with chemical control of developing larvae, adult trapping is an important control strategy. In this study, four blue-black fabric traps, Nzi, Vavoua, Model H, and Ngu, were compared with a white sticky trap currently used for stable fly control in Costa Rica. Overall, the white sticky trap caught the highest number of stable flies, followed by the Nzi, Vavoua, Model H, and Ngu. Collections on the white sticky trap increased 16 d after residues were chopped; coinciding with the expected emergence of flies developing in the pineapple residues. During this same time period, collections in the blue-black fabric traps decreased. Sex ratio decreased from >7:1 (females:males) 3-7 d after chopping to 1:1 at 24-28 d. White sticky, Nzi and Vavoua traps collected similar numbers of colonizing flies 3-7 d after residues were chopped. However, white sticky traps collected more flies once emergence from the pineapple residues began. Although white sticky traps collected more flies than fabric traps, they remain labor intensive and environmentally unsound because of their disposable and nonbiodegradable nature.

  18. Effects of Nitrogen Supplementation on Yeast (Candida utilis) Biomass Production by Using Pineapple (Ananas comosus) Waste Extracted Medium

    OpenAIRE

    Rosma, A.; Cheong, M. W.

    2007-01-01

    Pineapple waste medium was used to cultivate yeast, Candida utilis. It served as the sole carbon and energy source for the yeast growth. However, pineapple waste media contain very little nitrogen (0.003-0.015% w/v). Various nitrogen sources were incorporate and their effects on biomass, yield and productivity were studied. Significant (p

  19. [Transmembrane transport behavior of in vitro HepG2 cells of ananas and its effect on lipids and glucose distribution].

    Science.gov (United States)

    Pang, Yu-Nong; Chai, Yu-Shuang; Jiang, Jing-Fei; Wang, Xin-Pei; Yu, Xuan; Lei, Fan; Xing, Dong-Ming; Du, Li-Jun

    2014-08-01

    Pineapple (Ananas comosus) leaves contain mainly phenolic components with antioxidant and hypolipidemic effects. One of the principle components is p-coumaric acid. In this study, the transport behavior of p-coumaric acid, was observed after the administration of pineapple leaf phenols in vitro. Simultaneously, the effect of the phenols on glucose, total cholesterol and triglycerides transportation and metabolism in HepG2 cells was also observed. The results showed that the phenols had good transport characteristics. 5 min after the administration, p-coumaric acid of the phenols could be detected, and the content of p-coumaric acid reached the peak concentration after 60 min of the administration. p-coumaric acid of phenols have time-and dose-dependent manner. While promoting glucose transporter (GLUT4) and low density lipoprotein receptor (LDLR) expression, the phenols decreased intracellular lipid content. This reduction of intracellular lipid content was highly correlated with the promotion of lipoprotein lipase (LPL) and hepatic triglyceride lipase (HTGL) expression, while the reduction of intracellular glucose levels was correlated with glycogen synthesis in the cells.

  20. Occurrence of Bioactive compounds in Ananus comosus (L.):A quality Standardization by HPTLC

    Institute of Scientific and Technical Information of China (English)

    M.Kalaiselvi; D.Gomathi; C.Uma

    2012-01-01

    Objective: Ananus comosus (L.) is belonging to the family Bromeliaceae used to cure various diseases. The present study is focus to evaluate the HPTLC fingerprinting analysis and phytochemical analysis of Ananus comosus peel. Methods: HPTLC fingerprinting profiles was done by using Hamilton syringe and CAMAG LINOMAT 5 instrument. Results: Among all solvents, most of the secondary metabolites such as alkaloid, flavanoid, phenol, tannins, steroids etc were present in ethanolic extract of Ananus comosus peel and the yield of plant extract in different solvents were analyzed, from which ethanolic extract gave 2.40g/100g. The quantitative assay was also analyzed in flavanoid, alkaloid, phenols, carotenoid and lycopene. HPTLC fingerprinting profile confirms the presence of phenols, flavanoid and alkaloid. Conclusion:From the above results ethanolic extract of Ananus comosus peel can be used as therapeutic agents to treat various disorders caused by free radical and chemical substances due to presence of its secondary metabolites.

  1. Relationships in Ananas and other related genera using chloroplast DNA restriction site variation.

    Science.gov (United States)

    Duval, M F; Buso, G S C; Ferreira, F R; Noyer, J L; Coppens d'Eeckenbrugge, G; Hamon, P; Ferreira, M E

    2003-12-01

    Chloroplast DNA (cpDNA) diversity was examined using PCR-RFLP to study phylogenetic relationships in Ananas and related genera. One hundred fifteen accessions representing the seven Ananas species and seven other Bromelioideae including the neighboring monospecific genus Pseudananas, two Pitcairnioideae, and one Tillandsioideae were included in the study. Eight primers designed from cpDNA were used for generating fragments. Restriction by 18 endonucleases generated 255 variable fragments. Dissimilarities were calculated from the resulting matrix using the Sokal and Michener index and the neighbor-joining method was used to reconstruct the diversity tree. Phylogenetic reconstruction was attempted using Wagner parsimony. Phenetic and cladistic analyses gave consistent results. They confirm the basal position of Bromelia in the Bromelioideae. Ananas and Pseudananas form a monophyletic group, with three strongly supported sub-groups, two of which are geographically consistent. The majority of Ananas parguazensis accessions constitute a northern group restricted to the Rio Negro and Orinoco basins in Brazil. The tetraploid Pseudananas sagenarius joins the diploid Ananas fritzmuelleri to constitute a southern group. The third and largest group, which includes all remaining species plus some accessions of A. parguazensis and intermediate phenotypes, is the most widespread and its distribution overlaps those of the northern and southern groups. Ananas ananassoides is dominant in this sub-group and highly variable. Its close relationship to all cultivated species supports the hypothesis that this species is the wild ancestor of the domesticated pineapple. The data indicate that gene flow is common within this group and scarcer with both the first and second groups. Comparison of cpDNA data with published genomic DNA data point to the hybrid origin of Ananas bracteatus and support the autopolyploidy of Pseudananas. The Ananas-Pseudananas group structure and distribution are

  2. AVALIAÇÃO ECONÔMICA DA PRODUÇÃO DE ABACAXI (Ananas comosus L.) CULTIVAR PEROLA NA REGIÃO NORTE FLUMINENSE

    OpenAIRE

    Niraldo José Ponciano; Carlos Otávio Ribeiro Constantino; Paulo Marcelo de Souza; Edenio Detmann

    2006-01-01

    This work aimed to evaluate the economic viability of the pineapple production in the Northern region of Rio de Janeiro State. One concludes that the majority of producers in this region presented an internal return average rate of 33.08%, indicating that pineapple production shows satisfactory economic viability. On the other hand, several pineapple producers of this region are deficient in both production management and administration control. In this sense, it was observed low productivity...

  3. Purificação de bromelina dos resíduos de abacaxi (Ananas comosus L. Merril por sistemas bifásicos aquosos PEG/Sal

    Directory of Open Access Journals (Sweden)

    Diego de Freitas Coelho

    2011-01-01

    Full Text Available Due to the dimension of worldwide production of pineapple and to high amount of generated waste from its cultivation and processing, several research have been developed to recovery the enzymes presents on pineapple, such as bromelain. Bromelain is the general name given to the group of endopeptidases found in pineapple as well as all proteases derived from members of the family Bromeliaceae, which belongs to the pineapple. Because of that play important physiological roles, these enzymes offers a wide range of proven therapeutic applications. This work aims the purification of bromelain from pineapple waste through PEG/Salt aqueous two-phase systems (ATPS using (NH42SO4, CaCl2 and potassium phosphate. The results showed that in CaCl2 experiments have no ATPS formation in the range of its constituents. However, the PEG/phosphate systems and PEG/(NH42SO4 systems showed excellent results, recovering with bromelain purification factor of 7.4 and 10.7 times, respectively, and yields above 80%.

  4. Effect of cassava starch coating on quality and shelf life of fresh-cut pineapple (Ananas comosus L. Merril cv "Pérola").

    Science.gov (United States)

    Bierhals, Vânia S; Chiumarelli, Marcela; Hubinger, Miriam D

    2011-01-01

    This research studied the influence of treatment with ascorbic acid, citric acid, and calcium lactate dipping and cassava starch edible coatings on quality parameters and shelf life of fresh-cut pineapple in slices during 12 d at 5 °C. After previous tests, the treatments selected for this study were samples dipped into antibrowning solution with 0.5% of ascorbic acid and 1% of citric acid, with and without 2% of calcium lactate and coated with 2% of cassava starch suspensions. Changes in weight loss, juice leakage, mechanical properties (stress at failure), color parameters (L* and H*), ascorbic acid content, sensory acceptance, and microbial growth of fruits were evaluated. Samples only treated with antibrowning agents were used as control. Edible coatings with and without calcium lactate were efficient in reducing weight loss, juice leakage, and maintaining firmness during storage. However, these samples showed more browning and the ascorbic acid content was reduced. All treatments presented good sensory acceptance (scores above 6). The determining factor of shelf life of pineapple slices was the microbial spoilage. A shelf life of 8 d was obtained for pineapple slices only treated with antibrowning agents. On the other hand, coated samples showed a reduced shelf life of 7 d and higher yeast and mold growth. Thus, although cassava starch coatings were efficient in reducing respiration rate, weight loss, and juice leakage and maintained mechanical properties, these treatments were not able to increase the shelf life of minimally processed pineapple. Practical Application: Pineapple fruit is highly appreciated for its aroma, flavor, and juiciness, but its immediate consumption is difficult. Therefore, pineapple is a potential fruit for minimal processing. However, shelf life of fresh-cut pineapple is very limited by changes in color, texture, appearance, off-flavors, and microbial growth. The use of edible coatings as gas and water vapor barrier and antibrowning agents can extend the storage time and maintain the quality of fresh-cut produce. Cassava starch and alginate coatings are alternative to preserve minimally processed pineapples without changing the quality parameters of fresh fruit. Thus, this study is useful for consumers and fresh-cut industry interested in knowing factors affecting shelf life and quality of fresh-cut pineapple.

  5. Meta-análisis de los subproductos de piña (Ananas comosus para la alimentación animal.

    Directory of Open Access Journals (Sweden)

    Michael López-Herrera

    2014-07-01

    Full Text Available El objetivo del presente trabajo fue determinar el uso potencial de los sub-productos del cultivo de la piña para su empleo en la dieta de animales rumiantes. Se realizó una recopilación y análisis estadístico de la información obtenida de diferentes investigaciones realizadas hasta el año 2013 a nivel mundial, para la caracterización nutricional de la piña: planta entera, rastrojo (hojas, hijos y tallo, corona, corazón, cáscara y pulpa de la fruta, tallo y raíces, para optimizar su uso en la alimentación de animales rumiantes. Se comparó el efecto que tiene el manejo que se proporciona a los subproductos, siendo las variables de esta comparación, el proceso de ensilaje, el secado o el material fresco sin ningún tipo de aditivo. La composición nutricional de los materiales varió de acuerdo a la parte de la planta. Las raíces presentaron el mayor contenido de materia seca, fibra detergente neutro, fibra detergente ácido, lignina y cenizas. Mientras que las coronas y los rastrojos mostraron mayores contenidos de proteína cruda y energía, pero menor contenido de materia seca. Hubo una diferencia entre el proceso de deshidratado y el material fresco o ensilado, no así entre los materiales con alto contenido de humedad. Los subproductos obtenidos de los sistemas de producción de piña, tuvieron contenidos de energía y nutrimentos que permitirían su utilización como parte de la ración en la alimentación de rumiantes sin perjudicar su desempeño productivo.

  6. 菠萝基因组SSR分子标记的开发%Development of molecular markers from genomic-SSR of Pineapple (Ananas comosus)

    Institute of Scientific and Technical Information of China (English)

    童和林; 冯素萍; 陈友; 陈业渊; 孙光明; 武耀廷

    2010-01-01

    实验采用SAM法,以"台农17号"菠萝作为开发基因组SSR分子标记的植物材料,并对开发获得的SSR标记在16个菠萝品种中进行了验证.利用两对锚定引物和12对接头引物筛选菠萝基因组SAM文库,从中开发得到86条含有SSR位点的序列,对这86条序列用MISA软件搜索后得到94个SSR位点.其中单核苷酸重复3个,二核苷酸重复91个.二核苷酸重复仅有2种重复单元,一种是GM/CT,占二核苷酸重复总数的57.14%(52/91),另一种是AC/GT,占二核苷酸重复总数的42.86%(39,91).研究未发现三核苷酸及以上的重复单元.36对SSR引物对16个材料的扩增结果显示,24对引物能够扩增出清晰、重复性好且符合预期大小的条带,其中13对引物具有多态性,可成为下一步构建菠萝指纹图谱、遗传多样性分析及基因定位等研究中有用的SSR标记.

  7. Evaluation of genetic variability in micropropagated propagules of ornamental pineapple [Ananas comosus var. bracteatus (Lindley) Coppens and Leal] using RAPD markers.

    Science.gov (United States)

    Santos, M D M; Buso, G C S; Torres, A C

    2008-10-21

    The objective of the present study was to evaluate the genetic variability in micropropagated plantlets of ornamental pineapple, after the fourth period of subculture. The basal culture medium consisted of MS salts, vitamins, 3% sucrose, liquid formulation, supplemented with 6-benzylaminopurine (BAP) at concentrations of 0.125, 0.25, 0.5, 1.0, and 2.0 mg/L. The addition of BAP influenced the occurrence of genetic variation revealed using random amplified polymorphic DNA (RAPD) markers. Of a total of 520 primers tested, 44 were selected and amplified; 402 monomorphic bands (97.2%) and 18 polymorphic bands (2.8%) resulted among regenerated plantlets. The polymorphic fragments were produced by 12 primers (OPA-01, OPA-20, OPB-01, OPB-19, OPC-19, OPF-13, OPL-17, OPM-13, OPP-16, OPT-07, OPV-19, and OPX-03). Among the primers that identified polymorphism, OPA-01, OPA-20, OPB-19, OPC-19, OPL-17, OPP-16, and OPX-3 each showed, one polymorphic band and OPF-13 amplified a maximum of three bands. In this study, the RAPD technique was effective in showing the occurrence of somaclonal variations that occur during the micropropagation process of ornamental pineapple cultivation in BAP-supplemented medium, and it is possible to detect the presence of genetic variation in early stages of plant development.

  8. Empirical model based on Weibull distribution describing the destruction kinetics of natural microbiota in pineapple (Ananas comosus L.) puree during high-pressure processing.

    Science.gov (United States)

    Chakraborty, Snehasis; Rao, Pavuluri Srinivasa; Mishra, Hari Niwas

    2015-10-15

    High pressure inactivation of natural microbiota viz. aerobic mesophiles (AM), psychrotrophs (PC), yeasts and molds (YM), total coliforms (TC) and lactic acid bacteria (LAB) in pineapple puree was studied within the experimental domain of 0.1-600 MPa and 30-50 °C with a treatment time up to 20 min. A complete destruction of yeasts and molds was obtained at 500 MPa/50 °C/15 min; whereas no counts were detected for TC and LAB at 300 MPa/30 °C/15 min. A maximum of two log cycle reductions was obtained for YM during pulse pressurization at the severe process intensity of 600 MPa/50 °C/20 min. The Weibull model clearly described the non-linearity of the survival curves during the isobaric period. The tailing effect, as confirmed by the shape parameter (β) of the survival curve, was obtained in case of YM (β1) was observed for the other microbial groups. Analogous to thermal death kinetics, the activation energy (Ea, kJ·mol(-1)) and the activation volume (Va, mL·mol(-1)) values were computed further to describe the temperature and pressure dependencies of the scale parameter (δ, min), respectively. A higher δ value was obtained for each microbe at a lower temperature and it decreased with an increase in pressure. A secondary kinetic model was developed describing the inactivation rate (k, min(-1)) as a function of pressure (P, MPa) and temperature (T, K) including the dependencies of Ea and Va on P and T, respectively.

  9. Identification of volatiles from pineapple (Ananas comosus L.) pulp by comprehensive two-dimensional gas chromatography and gas chromatography/mass spectrometry.

    Science.gov (United States)

    Pedroso, Marcio P; Ferreira, Ernesto C; Hantao, Leandro W; Bogusz, Stanislau; Augusto, Fabio

    2011-07-01

    Combining qualitative data from the chromatographic structure of 2-D gas chromatography with flame ionization detection (GC×GC-FID) and that from gas chromatography-mass spectrometry (GC/MS) should result in a more accurate assignment of the peak identities than the simple analysis by GC/MS, where coelution of analytes is unavoidable in highly complex samples (rendering spectra unsuitable for qualitative purposes) or for compounds in very low concentrations. Using data from GC×GC-FID combined with GC/MS can reveal coelutions that were not detected by mass spectra deconvolution software. In addition, some compounds can be identified according to the structure of the GC×GC-FID chromatogram. In this article, the volatile fractions of fresh and dehydrated pineapple pulp were evaluated. The extraction of the volatiles was performed by dynamic headspace extraction coupled to solid-phase microextraction (DHS-SPME), a technique appropriate for slurries or solid matrices. Extracted analytes were then analyzed by GC×GC-FID and GC/MS. The results obtained using both techniques were combined to improve compound identifications.

  10. Influence of Soda Pulping Variables on Properties of Pineapple (Ananas comosus Merr. Leaf Pulp and Paper Studied by Face-Centered Composite Experimental Design

    Directory of Open Access Journals (Sweden)

    Jantharat Wutisatwongkul

    2016-01-01

    Full Text Available Face-centered composite design (FCC was used to study the effect of pulping variables: soda concentration (4-5 wt%, temperature (90–130°C, and pulping time (20–60 min on the properties of pineapple leaf pulp and paper employing soda pulping. Studied pulp responses were screened yield and lignin content (kappa number. Paper properties, which include tensile index, burst index, and tear index, were also investigated. Effects of the pulping variables on the properties were statistically analyzed using Minitab 16. The optimum conditions to obtain the maximum tensile index were soda concentration of 4 wt%, pulping temperature of 105°C, and pulping time of 20 min. The predicted optimum conditions provided tensile index, burst index, tear index, screened yield, and kappa number of 44.13 kN·m/kg, 1.76 kPa·m2, 1.68 N·m2/kg, 21.29 wt%, and 28.12, respectively, and were experimentally confirmed.

  11. Two phosphoenolpyruvate carboxykinases coexist in the Crassulacean Acid Metabolism plant Ananas comosus. Isolation and characterization of the smaller 65 kDa form.

    Science.gov (United States)

    Martín, Mariana; Rius, Sebastián Pablo; Podestá, Florencio Esteban

    2011-06-01

    Two phosphoenolpyruvate carboxykinase (PEPCK, EC 4.1.1.49) isoforms of 74 and 65 kDa were found to coexist in vivo in pineapple leaves, a constitutive Crassulacean Acid Metabolism plant. The 65 kDa form was not the result of proteolytic cleavage of the larger form since extraction methods reported to prevent PEPCK proteolysis in other plant tissues failed to yield a single immunoreactive PEPCK polypeptide in leaf extracts. In this work, the smaller form of 65 kDa was purified to homogeneity and physically and kinetically characterized and showed parameters compatible with a fully active enzyme. The specific activity was nearly twice higher for decarboxylation of oxaloacetate when compared to carboxylation of phosphoenolpyruvate. Kinetic parameters fell within the range of those estimated for other plant PEPCKs. Its activity was affected by several metabolites, as shown by inhibition by 3-phosphoglycerate, citrate, malate, fructose-1,6-bisphosphate, l-asparagine and activation of the decarboxylating activity by succinate. A break in the Arrhenius plot at about 30°C indicates that PEPCK structure is responsive to changes in temperature. The results indicate that pineapple leaves contain two PEPCK forms. The biochemical characterization of the smaller isoform performed in this work suggests that it could participate in both carbon and nitrogen metabolism in vivo by acting as a decarboxylase.

  12. Advance on research of blackheart in pineapple (Ananas comosus(L.)Merr.)%菠萝黑心病研究进展

    Institute of Scientific and Technical Information of China (English)

    张惠云; 吴青松; 孙伟生; 侍文博; 窦美安

    2009-01-01

    综述了菠萝黑心病的研究现状.菠萝黑心病的主要特征是菠萝果实果内褐变,酚类化合物、酶类、活性氧是褐变的发生必须具备的3个因素目前认为该病是受多种因素影响的一种复杂生理变化过程的病害.根据当前菠萝生产和保鲜情况,提出控制菠萝黑心病的途径.

  13. Studies into the phenolic patterns of different tissues of pineapple (Ananas comosus [L.] Merr.) infructescence by HPLC-DAD-ESI-MS (n) and GC-MS analysis.

    Science.gov (United States)

    Steingass, Christof B; Glock, Mona P; Schweiggert, Ralf M; Carle, Reinhold

    2015-08-01

    In a comprehensive study, more than 60 phenolic compounds were detected in methanolic extracts from different tissues of pineapple infructescence by high-performance liquid chromatography with diode array detection and electrospray ionisation multiple-stage mass spectrometry (HPLC-DAD-ESI-MS (n) ) as well as by gas chromatography-mass spectrometry (GC-MS). The analytical workflow combining both methods revealed numerous compounds assigned for the first time as pineapple constituents by their mass fragmentations. Pineapple crown tissue was characterised by depsides of p-coumaric and ferulic acid. In contrast, major phenolic compounds in pineapple pulp extracts were assigned to diverse S-p-coumaryl, S-coniferyl and S-sinapyl derivatives of glutathione, N-L-γ-glutamyl-L-cysteine and L-cysteine, which were also identified in the peel. The latter was additionally characterised by elevated concentrations of p-coumaric, ferulic and caffeic acid depsides and glycerides, respectively. Two peel-specific cyanidin hexosides were found. Elevated concentrations of isomeric N,N'-diferuloylspermidines may be a useful tool for the detection of fraudulent peel usage for pineapple juice production. Mass fragmentation pathways of characteristic pineapple constituents are proposed, and their putative biological functions are discussed.

  14. Effect of vitamin C in pineapple rind (Ananas comosus L. Merr on thyroxine hormone and anti stress on broilers in tropical region

    Directory of Open Access Journals (Sweden)

    Syahruddin E

    2013-03-01

    Full Text Available This study was aimed to determine the right level of vitamin C in extracted pineapple rind to prevent heat stress effects so it does not interfere with the performance of broiler chickens. This study was done in a series of field experiments. Experiments in the field/cage was biological test of the effect of vitamin C from pineapple rind on production responses (percentage of body weight gain and carcass and physiological responses (thyroxine hormone levels in broiler chicken aged 3 weeks as many as 360 of Strain Arbor acress. The basic design used was CRD 3 x 4 factorial models and 3 replications with 10 chickens for each box, as factor 1: Room temperature (21 : 27 and 33ºC, and factor II: level of vitamin C in the pineapple rind (0:500:1000 and 1500 ppm. The data obtained were statistically analyzed using SAS program package, and if it showed any significant effect then followed by Duncans test/DMRT. Variables measured were body weight gain, carcass percentage and levels of thyroxine hormone of broiler. Results showed that addition of pineapple rind containing 500 ppm vitamin C in the drinking water reduced heat stress in chicken that were kept at temperature of 27ºC, while at 33ºC needed 1000 ppm vitamin C. Both treatments increase level of thyroxine hormone, produce weight gain equal to control, more over, there was no effect on the percentage of carcasses.

  15. 无眼菠萝高效再生体系的研究%Study on Effective Regeneration System of Ananas comosus sp.

    Institute of Scientific and Technical Information of China (English)

    杨冬业; 张丽珍; 李伯林

    2011-01-01

    从工厂化生产角度出发,力求探索通过组织培养方式快速繁殖无眼菠萝种苗.以无眼菠萝茎为外植体建立无菌苗再生体系,结果表明:不定芽诱导培养基为:MS+6-BA2.0mg·L-1+NAA0.1mg·L-1;生根培养基为1/2MS+NAA0.1mg·L-1+IBA0.1mg·L-1;以泥炭:椰糠:珍珠岩:黄泥=4:1:1:2为基质,移栽成活率可达90%以上.

  16. Effects of ‘flowering chemicals’ application on the development of inflorescence and fruit of pineapple. [Ananas comosus (L. Merr. c.v. Phuket

    Directory of Open Access Journals (Sweden)

    Butrat, P.

    2005-05-01

    Full Text Available This comparative study of the effects of three different flowering chemicals, CaC2, ethephon, and NAA, on the development of inflorescence and fruit of pineapples c.v. Phuket was conducted at Amphoe Talang of Phuket Province, between December 2000 and September 2001. An experiment was arranged as a factorial design in RCB with 2 factors in 3 replications. In the first factor, there were CaC2 1 g/plant, ethephon 100 mg/l combenation with urea 1.5% 60 ml/plant, NAA 0.5 mg (1 ANAA tablet/plant, and the second factor was application period (one time and two times into the shoot tips of nine-month-old ground suckers. The results showed that the application of ethephon one time or two times led to faster floral development than in plants that received NAA or CaC2. In fact, the floral development with ethephon application was 5-7 days faster than that in NAA application, and 8-10 days faster than that in CaC2 application. Apical meristem differentiation took place 3 days after the application of ethephon, followed by complete development of the first floret and stamen within 42-43 days, anthesis within 45-46 days, and pistil within 48-49 days. Maturation of the egg was 5.7-6.3 days later than that of the pollen grains and 2.3-2.6 days after anthesis. In the treatment of NAA application once or twice, the development of the egg cell took a longer period.

  17. 金菠萝种苗组织培养技术%Tissue Culture Technology of Ananas comosus(L.)Merrill Seedling

    Institute of Scientific and Technical Information of China (English)

    符运柳; 徐立; 李志英

    2016-01-01

    根据对凤梨科植物离体保存和种苗繁育的生产实践经验,介绍了菠萝优良品种金菠萝组培苗一、二级苗的种苗生产技术,以促进该菠萝优良品种的推广种植.

  18. Analysis of Aroma Component of pineapple[ Ananas comosus (L.) Merr. ] with Gas Chromatography/Mass Spectrometry%菠萝香气成分的GC/MS分析

    Institute of Scientific and Technical Information of China (English)

    何应对; 魏长宾; 李绍鹏; 李瑞梅; 孙光明

    2007-01-01

    采用固相微萃取(SPME)方法提取成熟菠萝的芳香物质,经气相色谱-质谱联机(GC/MS)分析,结合计算机检索技术对分离化合物进行鉴定,应用色谱峰面积归一法测定各成分的相对含量.检测结果表明:共鉴定出29种芳香物质成分,约占总峰面积的91.53%,其中分离出烃类(8种,27.6%)、酯类(8种,27.6%)、酮类(3种,10.3%)、醇类(3种,10.3%)、醛类(2种,6.8%),还有酚、呋喃、酸、杂环、胺类各一种各占3.4%.为进一步评价菠萝品质和利用芳香物质提供基础数据.

  19. Taxonomic structure of the yeasts and lactic acid bacteria microbiota of pineapple (Ananas comosus L. Merr.) and use of autochthonous starters for minimally processing.

    Science.gov (United States)

    Di Cagno, Raffaella; Cardinali, Gainluigi; Minervini, Giovanna; Antonielli, Livio; Rizzello, Carlo Giuseppe; Ricciuti, Patrizia; Gobbetti, Marco

    2010-05-01

    Pichia guilliermondii was the only identified yeast in pineapple fruits. Lactobacillus plantarum and Lactobacillus rossiae were the main identified species of lactic acid bacteria. Typing of lactic acid bacteria differentiated isolates depending on the layers. L. plantarum 1OR12 and L. rossiae 2MR10 were selected within the lactic acid bacteria isolates based on the kinetics of growth and acidification. Five technological options, including minimal processing, were considered for pineapple: heating at 72 degrees C for 15 s (HP); spontaneous fermentation without (FP) or followed by heating (FHP), and fermentation by selected autochthonous L. plantarum 1OR12 and L. rossiae 2MR10 without (SP) or preceded by heating (HSP). After 30 days of storage at 4 degrees C, HSP and SP had a number of lactic acid bacteria 1000 to 1,000,000 times higher than the other processed pineapples. The number of yeasts was the lowest in HSP and SP. The Community Level Catabolic Profiles of processed pineapples indirectly confirmed the capacity of autochthonous starters to dominate during fermentation. HSP and SP also showed the highest antioxidant activity and firmness, the better preservation of the natural colours and were preferred for odour and overall acceptability.

  20. Sulfur-containing constituents and one 1H-pyrrole-2-carboxylic acid derivative from pineapple [Ananas comosus (L.) Merr.] fruit.

    Science.gov (United States)

    Zheng, Zong-Ping; Ma, Jinyu; Cheng, Ka-Wing; Chao, Jianfei; Zhu, Qin; Chang, Raymond Chuen-Chung; Zhao, Ming; Lin, Zhi-Xiu; Wang, Mingfu

    2010-12-01

    Two sulfur-containing compounds, (S)-2-amino-5-((R)-1-carboxy-2-((E)-3-(4-hydroxy-3-methoxyphenyl)allylthio)ethyl-amino)-5-oxopentanoic acid (1) and (S)-2-amino-5-((R)-1-(carboxymethylamino)-3-((E)-3-(4-hydroxyphenyl)allylthio)-1-oxopropan-2-ylamino)-5-oxopentanoic acid (2), and one 1H-pyrrole-2-carboxylic acid derivative, 6-(3-(1H-pyrrole-2-carbonyloxy)-2-hydroxypropoxy)-3,4,5-trihydroxy-tetrahydro-2H-pyran-2-carboxylic acid (3), together with eighteen known phenolic compounds, were isolated from the fruits of pineapple. Their structures were elucidated by a combination of spectroscopic analyses. Some of these compounds showed inhibitory activities against tyrosinase. The half maximal inhibitory concentration values of compounds 1, 4, 5, 6, 7 are lower than 1 mM. These compounds may contribute to the well-known anti-browning effect of pineapple juice and be potential skin whitening agents in cosmetic applications.

  1. 分子标记在菠萝研究中的应用%Application of Molecular Markers on Pineapple (Ananas comosus (L.) Merr.)

    Institute of Scientific and Technical Information of China (English)

    马帅鹏; 庞振才; 李静; 何时雨; 郑永清; 魏守兴

    2014-01-01

    介绍了分子标记技术在菠萝上的应用,主要包括种质鉴别、遗传多样性、亲缘关系、连锁图谱构建以及与优良农艺性状相关的分子标记的获得等方面的情况,并对存在的问题和应用前景进行了讨论。%This paper summarizes the application of molecular markers in the research of pineapple,mainly including identification, genetic diversity, genetic map construction, achievement of molecular markers that were closely linked to the target genes. At last, we discuss the problems and prospects of the application.

  2. The effect of different hormones and incubation periods on in vitro proliferation of pineapple (Ananas comosus L.) Merr cv. smooth cayenne) shoot-tip culture.

    Science.gov (United States)

    Hamad, Abdelhamid M; Taha, Rosna Mat

    2008-02-01

    Seven different hormone treatments, namely 6-benzylaminopurine (BAP) at 2, 3 mg L(-1) was applied singly and in combination with Indole Acetic Acid (IAA) at 0.18, 0.8 and 1.8 mg L(-l), BAP at 3.3 mg L(-l) in combination with IAA at 1.8 and 3.3 mg L(-l) and triple combination of BAP at 2.3, IAA at 1.8 and Gibberellic acid (GA3) at 1.0 mg L(-1) were tested, over four different incubation periods of 30, 45, 60 and 75 days, for their effect in the proliferation and growth of Smooth cayenne pineapple shoot-tip culture. Combined application of BAP at 3.3 and IAA at 1.8 mg L(-1) induced the highest proliferation of 19 shoots/explant and the highest total of 121 and 125 shoots over 4 cycles of multiplication. Raising the IAA to 3.3 mg L(-1) resulted in the lowest proliferation and stunted shoots. Incorporation of GA3 improved the shoot length but caused drastic reduction in proliferation. The other treatments showed an intermediate effect.

  3. 苹果菠萝的组织培养与快速繁殖%Tissue Culture and Rapid Propagation of Ananas comosus ‘Apple’

    Institute of Scientific and Technical Information of China (English)

    昝丽梅; 张家云; 吴维军

    2014-01-01

    以苹果菠萝的顶芽和吸芽为外植体,接种于MS+6-BA3 mg·L-1+蔗糖30g·L-1的培养基上,30 d后腋芽萌发;再将新芽切割,接种于MS+6-BA2 mg· L-1 +NAA0.05 mg· L-1+蔗糖30g·L-1的培养基上,30 ~40 d后形成丛生芽,每30~40 d继代1次,可增殖3~5倍;把丛生芽分割成单株并接种于MS+6-BA 1 mg·L-1 +AD3 mg·L-1+蔗糖30 g·L-1培养基上,进行壮苗培养,25 ~30 d后再转接种于1/2MS+NAA 0.5 mg· L-1+香蕉汁30g· L-1+蔗糖30g· L-1培养基上,30 d后可形成完整的根系;小植株移栽成活率可达95%.

  4. Identification of viral and non-viral reverse transcribing elements in pineapple (Ananas comosus), including members of two new badnavirus species.

    Science.gov (United States)

    Gambley, C F; Geering, A D W; Steele, V; Thomas, J E

    2008-01-01

    A previously published partial sequence of pineapple bacilliform virus was shown to be from a retrotransposon (family Metaviridae) and not from a badnavirus as previously thought. Two newly discovered sequence groups isolated from pineapple were associated with bacilliform virions and were transmitted by mealybugs. Phylogenetic analyses indicated that they were members of new badnavirus species. A third caulimovirid sequence was also amplified from pineapple, but available evidence suggests that this DNA is not encapsidated, but more likely derived from an endogenous virus.

  5. Avaliação da influência da temperatura e do tratamento enzimático no comportamento reológico do suco de abacaxi pérola (Ananas Comosus L. merr. Evaluation of influence from temperature and enzymatic treatment in the rheological behavior of pineapple (Ananas Comosus L. merr. juice

    Directory of Open Access Journals (Sweden)

    Adriano Cesar Calandrini Braga

    2013-03-01

    Full Text Available O objetivo deste trabalho foi determinar o comportamento reológico do suco de abacaxi- pérola natural e tratado com enzimas pectinolíticas. As condições de tratamento enzimático foram otimizadas através de um planejamento experimental do tipo fatorial completo 2k, com três repetições do ponto central. Na avaliação do comportamento reológico foram utilizadas duas amostras submetidas a peneiramento (N e D, analisadas em quatro diferentes temperaturas (10; 25; 50 e 65 °C. As análises reológicas foram realizadas utilizando um viscosímetro de cilindros concêntricos Brookfield e os dados experimentais foram ajustados ao modelo de Mizrahi-Berk. A relação entre temperatura e viscosidade aparente foi descrita por uma equação tipo Arrhenius. A otimização da atividade enzimática indicou, através da análise de variância e da metodologia de superfície de resposta, que as variáveis temperatura e tempo de tratamento exerceram efeito estatisticamente significativo (pThe objective this study was determinate the rheological behavior of pineapple juice natural and treated with pectinolytic enzymes. Tests for optimization of enzyme activity used in treatment were realized using a complete factorial planning 2k, with three repetitions of center point and two independent variables (temperature and time of treatment. In evaluation of rheological behavior of pineapple juice, were utilized two samples submitted to sieving (N and D, analyzed in four different temperatures (10, 25, 50 e 65 °C. The rheological analyses were realized using a Brookfield concentric cylinders viscometer and the relationship in the temperature and apparent viscosity was described by an equation type Arrhenius. The experimental data were adjusted by the Mizrahi-Berk model. The optimization tests indicated by ANOVA and response surface methodology that the variables temperature and time of treatment have statistically significant effect (p<0,05 on concentration of pectin present in the samples. The enzymatic treatment was carried with an enzyme concentration of 150 mg.L-1, temperature at 50 °C and treatment time of 80 minutes. The model used was adequate to describe the rheological behavior of the juices according to the parameters R², χ² e Bf. The reduced values of the behavior index (0.61-0.83 for natural juice and 0.57-0.72 for despectinized juice indicated the pseudoplastic behavior (n<1. The temperature influenced the yield stress and apparent viscosity of the juices analyzed, where the minors values were observed in samples treated at 65 °C, 2.4 mPa.s and 1.4 mPa.s, respectively, for the natural and despectinized samples. The equation of Arrhenius described satisfactorily the effect of temperature in the apparent viscosity. The activation energy values (Eat was of 4.54 Kcal.g.mol-1 and 4.89 Kcal.g.mol-1, respectively, for the natural and despectinized samples of pineapple juice, increasing with enzymatic treatment. The enzymatic treatment decreased the values of rheological behavior parameters for samples of pineapple juice, and the apparent viscosity for all temperatures used, where the largest percentage reduction was observed at 65 °C (41.66%.

  6. Condições de pH e temperatura para máxima atividade da bromelina do abacaxi (Ananas Comosus L. Merril = Temperature and pH conditions for maximum activity of bromelain extracted from pineapple (Ananas Comosus L. Merril

    Directory of Open Access Journals (Sweden)

    Moacyr Jorge Elias

    2011-04-01

    Full Text Available A bromelina, enzima presente no abacaxi, hidrolisa ligações peptídicas das proteínas; tem aplicação em diversas áreas envolvendo alimentos e medicina. Visando o aproveitamento da bromelina recuperada, a partir dos resíduos da industrialização do fruto(variedade pérola, foram pesquisadas condições de pH e temperatura para maior atividade, empregando caseína como substrato. O extrato foi obtido pela trituração da casca e do talo interno do fruto. A atividade foi expressa em mmol tirosina L-1 min.-1 pela absorbância a280 nm dos aminoácidos aromáticos gerados na hidrólise da caseína. Os ensaios foram efetuados em duplicata para duas relações enzima/substrato: 1/25 e 1/125 (em massa. Foi estabelecido um planejamento experimental em estrela tendo como ponto central pH em7,0 e temperatura de 35°C. Os resultados foram tratados empregando o aplicativo computacional MINITAB 15 da Minitab Inc. que forneceu as equações do modelo e as superfícies de resposta. As equações foram tratadas matematicamente (cálculo diferencial, fornecendo gráficos da melhor atividade em função da temperatura. Observou-se perda daatividade enzimática com a elevação da temperatura para relação 1/25, atribuída à maior presença de enzima livre em comparação com a relação 1/125.Bromelain, the enzyme found in pineapple, hydrolyzes proteins’ peptide bonds, has several applications in the food industry and in medicine. Temperature and pH conditions for highest activity with casein as substrate were investigated from the bromelain recovered from industrialized pineapple (‘pérola’ variety fruit residues. Extract was obtained by grinding fruit rind and its internal stem. The activity was expressed in mmol tyrosine L-1 min.-1 by absorbance at 280 nm of aromatic amino acids produced in casein hydrolysis. Assays were undertaken in duplicate for two enzyme/substrate with ratios: 1/25 and 1/125 (mass. Eight-pointed experimental design was undertaken with central value for pH at 7.0 and for temperature at 35°C. Experimental results were processed by MINITAB 15 (Minitab Inc software which provided model’s equations and surface responses. Equations were mathematically processed by differential calculus producing graphs which exhibit the best activities according to temperatures. Loss of enzyme activity was reported with temperature increase at 1/25 ratio, due to a higher free enzyme, when compared to 1/125 ratio.

  7. Características da atividade da peroxidase de abacaxis (Ananas comosus (L. Merrill da cultivar IAC Gomo-de-mel e do clone IAC-1 Biochemical characteristics peroxidase from pineapple (Ananas comosus (L. Merrill: cultivar IAC Gomo-de-mel and clone IAC-1

    Directory of Open Access Journals (Sweden)

    Carlos Alexandre Koguishi de Brito

    2005-06-01

    Full Text Available No presente trabalho foram estudadas as características bioquímicas das peroxidases de novos abacaxis, cultivar IAC Gomo-demel e clone IAC-1. As peroxidases dos sucos destes abacaxis apresentaram atividade ótima entre 45ºC e 50°C e entre 50°C e 55°C, respectivamente. Estas peroxidases apresentaram atividade ótima em pH 4,5 e mostraram-se estáveis na faixa de pH 4,0 a 9,0, retendo, após 24 horas de incubação a 50ºC, mais de 80% da atividade inicial. Foi observada regeneração parcial da atividade da peroxidase, após tratamento a 75ºC por 10 minutos. As peroxidases dos sucos dos abacaxis estudados foram inativadas, após tratamento a 90ºC por 2 minutos.In the present work, the biochemical characteristics of peroxidase enzymes from new pineapples, cultivar IAC Gomo-de-mel and clone IAC-1, were studied. The peroxidases obtained from the juice of this pineapples presented optimum activities from 45 ºC to 50 ºC and 50 ºC to 55 ºC, respectively. These peroxidases showed maximum activity at pH 4.5 and showed stability at pH 4.0 to 9.0, retaining after 24 hours of incubation at 50 ºC more than 80% of initial activity. Partial regeneration of peroxidase activity was observed after treatment at 75 ºC for 10 minutes. The peroxidases from pineapple juices studied were inactivated after treatment at 90 ºC for 2 minutes.

  8. Evaluation of the effects of gamma radiation on the quality of pineapple (Ananas comosus (L.) Meer) cv. Smooth Cayenne minimally processed, storaged on differents temperatures and packages;Avaliacao dos efeitos da radiacao gama na qualidade de abacaxi (Ananas comosus (L.) Meer) cv. Smooth Cayenne minimamente processado, armazenado em diferentes temperaturas e embalagens

    Energy Technology Data Exchange (ETDEWEB)

    Leite, Daniela Terenzi Stuchi

    2006-07-01

    The present work aimed to verify the effects of gamma radiation (doses until 2 kGy), types of packages and temperatures of storage (5, 8 e 12 deg C) on the physicochemical characteristics, on the microbiological contamination and on the sensorial characteristics of pineapple 'Smooth Cayenne' minimally processed. The fruits were selected, washed; peeled and cutted transverse and the slices cutted were cutted on two or for pieces. The pieces were immersed in chlorinated water (100 mg/L) for 3 minutes, flowing and package, irradiated and stored. According with the results obtained in thi present work it was concluded that bigger the temperature of storage more quickly were the browning of the fruits. The loss of fresh weight of pineapple was bigger in the packages of polystyrene comparing with PET package when both are covered with PVC film and smaller in the PET packages covered with the same material. Doses of gamma radiation until 2 kGy did not change the physico-chemical and sensorial characteristics of pineapple 'Smooth Cayenne' minimally processed. The microbiological growth on the pieces of the processed fruit was smaller on the biggest dose. All the samples were is in good conditions for the human consume. (author)

  9. EFFECTS OF SUBSTRATUM AND CONTAINERS IN ACCLIMATION OF PINEAPPLE SEEDLINGS [Ananas comosus (L. Merril] CV. PÉROLA EFEITOS DE SUBSTRATOS E RECIPIENTES NA ACLIMATAÇÃO DE PLÂNTULAS DE ABACAXIZEIRO [Ananas comosus (L. Merril] CV. PÉROLA

    Directory of Open Access Journals (Sweden)

    Sarah Brandão Santa Cruz Barboza

    2007-09-01

    Full Text Available

    After the removal of the in vitro culture medium, the survival and growth of micropropagated seedlings are among the main difficulties found in several crops. The objective of this study was to evaluate the behavior of pineapple cv. Pérola seedlings produced in vitro and acclimated in different substrates and containers. The experimental design was completely randomized split plot, with four replicates. The plots (twenty plants consisted of three substrates (sand/plant fiber/humus, Plantmax and sand and the subplots consisted of three containers (a tubet 5cm in diameter by 13cm high, a tubet 5cm in diameter by 24.5cm high and a 10cm x 8cm plastic bag. The combinations of sand/plant fiber/humus with 5x13cm tubet or with a plastic bag, and of Plantmax with 5x13cm tubet provide better growth in ex vitro conditions.

    KEY-WORDS: Culture in vitro; micropropagation; fruitculture.

    A sobrevivência e o crescimento de plântulas micropropagadas, após a remoção do meio de cultivo in vitro, estão entre as principais dificuldades encontradas em várias culturas. O objetivo deste trabalho foi avaliar o comportamento de plântulas de abacaxi cv. Pérola, produzidas in vitro e submetidas a aclimatação em diferentes substratos e recipientes. O delineamento experimental foi inteiramente ao acaso em parcelas subdivididas, com quatro repetições. As parcelas (com vinte plântulas foram constituídas de três substratos (areia/xaxim/húmus, Plantmax e areia e as subparcelas, de três recipientes (tubete com 5cm de diâmetro x 13cm de altura, tubete com 5cm de diâmetro x 24,5cm de altura e saco plástico, com 10cm x 8cm. As combinações areia/xaxim/húmus com tubete pequeno ou com saco plástico e Plantmax com tubete de 5cm diâmetro x 13cm de altura proporcionaram melhores respostas ao crescimento em condições ex vitro.

    PALAVRAS-CHAVE: Cultivo in vitro; micropropagação; fruticultura; parcelas subdivididas.

  10. PRESENCIA DEL ZORRO DE CUATRO OJOS (Philander opossum EN EL CULTIVO DE PIÑA (Ananas comusus

    Directory of Open Access Journals (Sweden)

    Javier Monge-Meza

    2010-01-01

    Full Text Available El objetivo de este trabajo fue determinar la presencia del zorro de cuatro ojos (Philander opossum en áreas de producción de piña orgánica (Ananas comusus, en Sa nta Ce cilia de La Cruz, Guanacaste, Costa Ri ca. Las capturas se realizaron con trampas grandes de golpe, en muestreos quincenales desde febrero del 2008 hasta febrero del 2009. Se logró la captura de ocho individuos, desde marzo hasta agosto, lo cual indica que la especie podría tener actividad reproductiva desde finales de la época seca y en la lluviosa. Al menos uno de los individuos consumió piña, según análisis de su contenido estomacal. Si n embargo, el nivel de daño observado en el campo no permite considerar a esta especie como una plaga de la piña, sino una especie oportunista que aprovecha frutos maduros dejados en el campo, después de la cosecha. La colecta de individuos en varios meses del año, indican que esta especie utiliza las plantaciones de piña orgánica como parte de su hábitat, lo cual contradice la idea generalizada que los monocultivos no proveen condiciones mínimas para que las especie s de vida silvestre lo visiten o lo habiten.

  11. Material science examination on pineapple fruit puree and raw material

    OpenAIRE

    Uan-On, Tanat

    2009-01-01

    Dem Ananas (Ananas comosus (L.) Merr) kommt unter den kommerziellen, tropischen Früchten eine große Bedeutung zu. Normalerweise wird Ananaspüree als Sterilkonserve in Dosen oder durch als Gefrierkonserviert. Ein Frischeprodukt in Pureeform ist nicht bekannt. Allerdings treten Probleme während der Lagerung oder dem Mischen auf. Die mangelnde Stabilität führt zu einer Trennung in Fasern und Serum. Das Ziel dieser Arbeit war es, die Stabilität des Ananaspürees durch Verwendung verschiedener Hydr...

  12. Physicochemical, Proximate and Sensory Properties of Pineapple (Ananas sp.) Syrup Developed from Its Organic Side-Stream

    NARCIS (Netherlands)

    Tortoe, C.; Johnson, P.N.T.; Slaghek, T.; Miedema, M.; Timmermans, T.

    2013-01-01

    A major economical industrial challenge from pineapple (Ananas sp.) processing contributing to environmental pollu- tion is the organic side-streams of pineapple. The physicochemical, proximate and sensory properties of organic side- stream pineapple syrup (OSPS) developed from Smooth cayenne, Sugar

  13. Physicochemical, Proximate and Sensory Properties of Pineapple (Ananas sp.) Syrup Developed from Its Organic Side-Stream

    OpenAIRE

    Tortoe, C.; Johnson, P.N.T.; Slaghek, T.; Miedema, M.; Timmermans, T.

    2013-01-01

    A major economical industrial challenge from pineapple (Ananas sp.) processing contributing to environmental pollu- tion is the organic side-streams of pineapple. The physicochemical, proximate and sensory properties of organic side- stream pineapple syrup (OSPS) developed from Smooth cayenne, Sugar loaf and MD2 pineapple varieties were evalu- ated. Organic side-stream pineapple syrup developed from MD2 recorded the highest moisture content with a corre- sponding water activity. The colour ch...

  14. Aplicación y comparación de pretratamientos totalmente libres de cloro en residuos de piña (Ananas comosus) y zapote mamey (Pouteria sapota) para la obtención de carboximetilcelulosa

    OpenAIRE

    José Manuel Juárez Barrientos; Emmanuel de Jesús Ramírez Rivera; Enrique Ramírez Figueroa; Lorena Guadalupe Ramón Canul; Jesús Rodríguez Miranda

    2011-01-01

    Derivado del aprovechamiento de la piña y el zapote mamey en la región de Tuxtepec, Oaxaca, México, se generan grandes cantidades de residuos, siendo estos en su mayoría materiales lignocelulósicos compuestos principalmente por celulosa, hemicelulosa y lignina a través de los cuales es posible obtener derivados como la carboximetilcelulosa (CMC) aislando previamente la fracción celulósica de los demás componentes. Estos residuos fueron caracterizados previamente en base a un análisis químico ...

  15. Fermentados alcoólico e acético de polpa e casca de abacaxi (Ananas Comosus (L.) Merril): cinética das fermentações e caracterização dos produtos

    OpenAIRE

    Leticia Mendonca Alvarenga

    2014-01-01

    O Brasil é um grande produtor de frutas, apesar dos elevados índices de perdas. Produtos obtidos por fermentações alcoólica e acética de resíduos e excedentes da produção de frutas são alternativas para minimizar as perdas provenientes da pós-colheita e da indústria. A qualidade de um produto fermentado está relacionada com a sua composição química, a tecnologia empregada no processamento e a matéria-prima utilizada na sua elaboração. Os objetivos deste trabalho foram a avaliação físico-quími...

  16. Obtención de Preparados Enzimáticos a Partir de Tallos de Piña (Ananas Comosus) con Potencialidades de uso en la Biotecnología y la Medicina.

    OpenAIRE

    Martha Hernández; Carol Carvajal; Margarita Márquez; Roxana Báez; Humberto Morris; Ramón Santos; María de los Ángeles Chávez

    2005-01-01

    El aislamiento y purificación de productos naturales a partir de plantas es una vertiente muy importante de la biotecnología moderna. La bromelina (EC 3.4.22.32) es una proteasa aislada de plantas de piña que se ha empleado tradicionalmente en la industria alimenticia. La enzima también se ha usado en la industria farmacéutica. En la actualidad existe un renovado interés por ella, desde que fue descrita su actividad antitumoral y antimetastizante frente a algunos tumores. En este estudio, se ...

  17. “extracción, purificación parcial y secado de la enzima bromelina obtenida a partir del corazón de la piña (ananas comosus)”.

    OpenAIRE

    Quinde Fuentes, Carmina Stefania; Sánchez Lluguín, Nancy Viviana

    2013-01-01

    El presente artículo tuvo como objetivo la purificación parcial de la enzima bromelina mediante el sistema bifásico acuoso (SBA), evaluando el efecto del pH y las concentraciones de los componentes PEG 8000 y sales fosfato del sistema. La bromelina fue extraída del corazón de la piña de la variedad Milagreña mediante solución buffer a 4°c con EDTA, cisteína y fosfato disódico anhídrido. El mejor resultado de purificación obtenido en el sistema bifásico acuoso fue el que consistió de 16% PEG, ...

  18. 卡因菠萝N、P、K养分累积规律的研究%Study on the Accumulation Pattern of N,P,K of Ananas comosus Plant

    Institute of Scientific and Technical Information of China (English)

    陈菁; 孙光明; 臧小平; 冼皑敏

    2010-01-01

    以卡因菠萝为试验材料,研究了其11个生长发育时段各部位各器官的生物量和N、P、K养分含量,以揭示N、P、K养分的累积规律.结果表明:①从植后至植后352 d,植株N、P、K累积速率基本呈上升趋势,植后352d至397d,N累积速率有所下降,P继续上升,K保持不变.植后397 d至564 d(谢花期),植株N、P、K累积速率逐步下降,此后至655 d(果实发育后期),N、P、K累积速率迅速上升,并达到最大值,此后至收获期逐步下降;②在菠萝各个器官中,叶片N、P、K累积速率在花芽分化前与整株N、P、K累积规律相同,此后其累积速率开始下降,在果实发育后期已降为负值.茎N、P、K累积速率在植后352d至564d(谢花期)累积较快,根N、P、K在植后201 d至277 d间和352 d至397 d间累积速率较快,果柄N、P、K在果实发育前中期、果实N、P、K在果实发育后期,芽N、P、K在果实发育后期累积速率最快;③生长前期,植株N、P、K的累积量较少,快速生长期则累积较多,果实生长发育期还有一个N、P、K累积高峰;④收获期,每公顷卡因菠萝植株累积的N、P、K分别为282.4、30.4、573.2 kg.

  19. 红掌和金边凤梨茎基腐病菌鉴定%Identification of stem rot and root rot pathogens isolated from Anthurium andraeanum and Ananas comosus var. variegata

    Institute of Scientific and Technical Information of China (English)

    黄江华; 宾淑英; 向梅梅; 周晓云; 王丽平

    2008-01-01

    多年生常绿草本花卉红掌Anthurium andraea-hum[1]和风梨具有很高的观赏和经济价值,是近年来流行的室内盆栽植物[2]。红掌的主要致病菌有寄生疫霉Phytophthora parasitica、棘腐霉Pythium pringsheim、茄病镰刀菌Fusarium solani、尖孢镰刀菌Fusarium oxysporum、

  20. 菠萝皮渣多糖的提取与体外抗氧化活性研究%Extraction and in vitro Antioxidant Activities of Polysaccharides from Ananas comosus (L.) Merr. Peel

    Institute of Scientific and Technical Information of China (English)

    罗建平; 徐学玲; 潘利华; 崔源

    2009-01-01

    研究菠萝皮渣多糖的提取工艺参数,并对提取的多糖进行体外抗氧化活性的评价.单因素试验和三因素二次通用旋转组合设计试验表明,菠萝皮渣多糖的最佳提取工艺为料液比1:25(m/V)、提取时间5h、提取温度70℃.提取的多糖具有一定的抗氧化活性,对O-2·和ABTS自由基的清除率表现为显著的量效关系,清除O2-·和ABTS自由基的半抑制率(IC50)分别为1.76mg/ml和2.49mg/ml.

  1. Genetic Diversity Analysis of Pineapple(Ananas comosus (L.)Merr) Germplasm with SCoT Molecular Markers%菠萝种质目标起始密码子(SCoT)遗传多样性分析

    Institute of Scientific and Technical Information of China (English)

    王健胜; 贺军虎; 陈华蕊; 陈业渊; 乔飞

    2015-01-01

    利用SCoT分子标记技术对来自9个国家或地区的46份菠萝种质进行了遗传多样性分析,并对SCoT标记在菠萝研究中的效率做了探讨.结果表明,SCoT标记在菠萝种质中具有丰富的多态性,引物多态性条带百分比在75% ~ 100%之间,平均为94.61%;引物的有效等位基因数(Ne)、Nei's基因多样性指数(H)、Shannon's信息指数(,)和多态性信息含量(PIC)平均值分别为1.45、0.27、0.41和0.28,表明SCoT标记具有较高的多态性检测效率.基于SCoT标记计算获得的遗传相似系数对菠萝种质做聚类分析,46份菠萝种质可被划分为5个类群,其中,第Ⅰ类群所包含的菠萝种质数量最多,占菠萝种质总数的84.78%.主成分分析获得了与聚类分析不尽一致的结论,但两者反映的种质亲缘关系基本一致.本研究结果将为我国菠萝种质的鉴定、保存和科学利用提供一定的理论依据.

  2. Antibacterial activities and stability of the ethanolic extract from the leaves of Ananas comosus%菠萝叶乙醇提取物的抑菌活性及其稳定性研究

    Institute of Scientific and Technical Information of China (English)

    黄筱娟; 纪明慧; 舒火明; 郭飞燕; 董力玮; 翟富荣

    2014-01-01

    采用96孔板法和倾注平板法研究菠萝叶总提物及其不同溶剂萃取物的抑菌活性,同时考察温度、光照和pH对乙酸乙酯萃取物抑菌活性稳定性的影响.结果表明,乙酸乙酯萃取物的抑菌效果最佳,其对金黄色葡萄球菌、蜡状芽孢杆菌、枯草杆菌、藤黄八叠球菌的最小抑菌浓度(MIC)分别为3.12、3.12、6.25、6.25mg/mL,对黑曲霉和青霉的最小抑菌浓度(MIC)分别为5、10mg/mL.在设定的温度范围、光照条件和不同酸度条件下,乙酸乙酯萃取物的抑菌活性较稳定.因此,菠萝叶乙酸乙酯萃取物的抑菌活性具有一定的光热稳定性和酸稳定性.

  3. The effects of concentrate added to pineapple (Ananas comosus Linn. Mer.) waste silage in differing ratios to form complete diets, on digestion, excretion of urinary purine derivatives and blood metabolites in growing, male, Thai swamp buffaloes.

    Science.gov (United States)

    Jetana, T; Suthikrai, W; Usawang, S; Vongpipatana, C; Sophon, S; Liang, J B

    2009-04-01

    Four, male, growing Thai swamp buffaloes (197 +/- 5.3 kg and all 1 year old) were used to evaluate the effects of concentrate added to pineapple waste silage in differing ratios, to form a complete diet, studying in vivo digestion, the rate of passage, microbial protein synthesis and blood metabolites. Animals were fed ad libitum with 4 diets, using four combinations of pineapple waste silage (P) and concentrate (C), in the proportions (on a dry matter basis) of 0.8:0.2 (P80:C20), 0.6:0.4 (P60:C40), 0.4:0.6 (P40:C60) and 0.2:0.8 (P20:C80). The results showed that the intakes of dry matter (DM), organic matter (OM), nitrogen (N), the N-balance, urinary purine derivatives (PD) excretion, the ratios of allantoin to creatinine (CR), PD to CR, the plasma urea-N (PUN) and insulin increased in the animals, but the intake of neutral detergent fiber (NDF), the coefficient of whole tract, apparent digestibility of NDF, the transit time (TT) and the mean retention time (TMRT) decreased, when the proportion of concentrate in the diet increased. This study indicated that the proportion of P40:C60 in the diet produced the best efficiency of urinary PD excretion (mmol) per digestible OM intake (kg DOMI).

  4. 47份菠萝种质遗传多样性的RAPD分析%Genetic Diversity Analysis of Forty Seven Pineapple [Ananas comosus (L.) Merr] Germplasm with RAPD Marker

    Institute of Scientific and Technical Information of China (English)

    王健胜; 贺军虎; 陈华蕊; 陈业渊

    2015-01-01

    利用RAPD分子标记对来自9个不同国家或区域47份菠萝种质的遗传多样性进行分析,结果表明,RAPD标记在菠萝种质中具有较好的多态性,引物多态性条带百分比平均达到82.26%,引物的有效等位基因数(Ne)、Nei's基因多样性指数(H)、Shannon's信息指数(I)和多态性信息含量(PIC)表现都较好,其平均值分别为1.46、0.26、0.39和0.24.基于RAPD分子检测数据对菠萝种质进行聚类分析,在GS=0.66水平上,47份菠萝种质被划分为4大类群.菠萝种质的主成分分析获得了与聚类分析基本一致的结论.

  5. Ripening-dependent metabolic changes in the volatiles of pineapple (Ananas comosus (L.) Merr.) fruit: II. Multivariate statistical profiling of pineapple aroma compounds based on comprehensive two-dimensional gas chromatography-mass spectrometry.

    Science.gov (United States)

    Steingass, Christof Björn; Jutzi, Manfred; Müller, Jenny; Carle, Reinhold; Schmarr, Hans-Georg

    2015-03-01

    Ripening-dependent changes of pineapple volatiles were studied in a nontargeted profiling analysis. Volatiles were isolated via headspace solid phase microextraction and analyzed by comprehensive 2D gas chromatography and mass spectrometry (HS-SPME-GC×GC-qMS). Profile patterns presented in the contour plots were evaluated applying image processing techniques and subsequent multivariate statistical data analysis. Statistical methods comprised unsupervised hierarchical cluster analysis (HCA) and principal component analysis (PCA) to classify the samples. Supervised partial least squares discriminant analysis (PLS-DA) and partial least squares (PLS) regression were applied to discriminate different ripening stages and describe the development of volatiles during postharvest storage, respectively. Hereby, substantial chemical markers allowing for class separation were revealed. The workflow permitted the rapid distinction between premature green-ripe pineapples and postharvest-ripened sea-freighted fruits. Volatile profiles of fully ripe air-freighted pineapples were similar to those of green-ripe fruits postharvest ripened for 6 days after simulated sea freight export, after PCA with only two principal components. However, PCA considering also the third principal component allowed differentiation between air-freighted fruits and the four progressing postharvest maturity stages of sea-freighted pineapples.

  6. Influence of harvest maturity and fruit logistics on pineapple (Ananas comosus [L.] Merr.) volatiles assessed by headspace solid phase microextraction and gas chromatography-mass spectrometry (HS-SPME-GC/MS).

    Science.gov (United States)

    Steingass, Christof B; Grauwet, Tara; Carle, Reinhold

    2014-05-01

    Profiling of volatiles from pineapple fruits was performed at four ripening stages using headspace solid-phase microextraction and gas chromatography-mass spectrometry (HS-SPME-GC/MS). In total, 142 volatiles were detected, of which 132 were identified. Multivariate data analysis was carried out to assess the effect of post-harvest storage on volatiles composition of green-ripe sea-freighted pineapple in comparison to air-freighted fruits harvested at full maturity. The latter fruits were characterised by volatiles described as potent odorants in pineapples, such as δ-octalactone, γ-lactones, 1-(E,Z)-3,5-undecatriene and 1,3,5,8-undecatetraene, as well as various methyl esters. In contrast, post-harvest storage of green-ripe sea-freighted fruits resulted in an increased formation of ethyl esters, acetates, acetoxy esters and alcohols, thus allowing the authentication of sea- and air-freighted pineapples, respectively. Particularly, compounds presumably derived from methyl-branched amino acid catabolism were identified in the fruits at later post-harvest stages. In addition, physicochemical traits were determined to characterise the fruit maturity stages.

  7. Cloning and analysis of AcSERK1 promoter from Ananas comosus L.‘Shenwan’%菠萝AcSERK1启动子的克隆及其初步活性分析

    Institute of Scientific and Technical Information of China (English)

    林文秋; 陈程杰; 何业华; 栾爱萍; 张俊丽; 冯筠挺; 张雅芬; 许文天

    2015-01-01

    [目的]探讨AcSERK1启动子的活性,为进一步研究AcSERK1转录调控的分子机制提供依据.[方法]利用hiTAIL-PCR法从菠萝基因组DNA中分离AcSERK1启动子序列,并利用Plant Care和PLACE分析该序列的顺式作用元件.将启动子与GUS融合,农杆菌真空渗透法浸染烟草叶片后,光照处理、暗处理和0.5 mg·L-1 2,4-D处理36 h后利用组织化学染色法检测GUS活性.[结果]克隆了AcSERK1上游2097bp启动子序列,命名为PAcsERK1.预测结果显示,该序列除含有TATA-box、CAAT-box等真核生物启动子核心元件外,还含有生长素、赤霉素和茉莉酸甲酯等激素响应元件,高温和低温胁迫响应元件以及光响应元件.瞬时表达结果表明,在光和2,4-D的诱导下PA.一1具有启动基因表达的功能.[结论]分离获得了AcSERK1启动子序列且该启动子在光和2,4-D诱导下能驱动GUS的表达.

  8. Metabolite Levels in the Chlorenchyma and the Water Storage Parenchyma of Ananas comosus Leaves%菠萝叶片绿色组织与贮水组织中代谢物水平的昼夜变化

    Institute of Scientific and Technical Information of China (English)

    陈产松; 齐一萍; 等

    2001-01-01

    研究了景天酸代谢(CAM)植物菠萝叶片绿色组织与贮水组织(WSP)的苹果酸、柠檬酸、异柠檬酸、淀粉、果糖、葡萄糖、蔗糖、葡糖-1-磷酸(G-1-P)、葡糖-6-磷酸(G-6-P)、果糖-6-磷酸(F-6-P)、草酰乙酸(OAA)及磷酸烯醇式丙酮酸(PEP)水平的昼夜变化。夜间苹果酸的积累仅发生在绿色组织中,表明只有绿色组织才能进行CAM。可溶性已糖(葡萄糖和果糖)是绿色组织中夜间苹果酸累积的主要碳源。绿色组织G-1-P、G-6-P和F-6-P水平在夜间的初期上升,后期下降,昼间的头3 h仍下降,3 h后变化不明显。绿色组织中OAA和PEP水平也发生昼夜变化。在贮水组织中没有测到淀粉、蔗糖、OAA和PEP。除葡萄糖和果糖外,WSP中其它代谢物的含量都远低于绿色组织,而且WSP中所有代谢物都无明显的昼夜变化。

  9. 菠萝叶中防晒成分的超声提取及其稳定性研究%Study on ultrasonic-assisted extraction and stability of the sunscreen constituents from leave sof Ananas comosus

    Institute of Scientific and Technical Information of China (English)

    郭飞燕; 纪明慧; 陈光英; 董力玮; 陈兰

    2016-01-01

    采用超声辅助提取法从菠萝叶中提取防晒成分,以单因素实验考察防晒成分的最佳提取工艺条件,并且研究了提取液中防晒成分的稳定性.结果表明:以丙酮为溶剂,料液比0.1200∶25(g·mL-1),提取温度50℃、提取时间90min、超声功率144W的条件下菠萝叶中防晒成分提取效果较佳,温度和光照对提取液的防晒活性没有明显的影响,菠萝叶中含有较强的紫外线吸收成分,是具有广谱防晒作用的植物.

  10. Avaliação da influência da temperatura e do tratamento enzimático no comportamento reológico do suco de abacaxi pérola (Ananas Comosus L. merr.

    Directory of Open Access Journals (Sweden)

    Adriano Cesar Calandrini Braga

    2013-03-01

    Full Text Available O objetivo deste trabalho foi determinar o comportamento reológico do suco de abacaxi- pérola natural e tratado com enzimas pectinolíticas. As condições de tratamento enzimático foram otimizadas através de um planejamento experimental do tipo fatorial completo 2k, com três repetições do ponto central. Na avaliação do comportamento reológico foram utilizadas duas amostras submetidas a peneiramento (N e D, analisadas em quatro diferentes temperaturas (10; 25; 50 e 65 °C. As análises reológicas foram realizadas utilizando um viscosímetro de cilindros concêntricos Brookfield e os dados experimentais foram ajustados ao modelo de Mizrahi-Berk. A relação entre temperatura e viscosidade aparente foi descrita por uma equação tipo Arrhenius. A otimização da atividade enzimática indicou, através da análise de variância e da metodologia de superfície de resposta, que as variáveis temperatura e tempo de tratamento exerceram efeito estatisticamente significativo (p<0,05 sobre a concentração de pectina presente na amostra. O modelo utilizado mostrou-se adequado para descrever o comportamento reológico dos sucos de acordo com os parâmetros R², χ² e Bf. Os baixos valores obtidos para o índice de comportamento indicaram um comportamento pseudoplástico (n<1. A temperatura exerceu influência sobre a tensão de cisalhamento e a viscosidade aparente dos sucos analisados, sendo os menores valores observados nas amostras analisadas a 65 °C. A equação tipo Arrhenius descreveu de modo satisfatório o efeito da temperatura sobre a viscosidade aparente. Os valores da energia de ativação (Eat foram de 4,54 Kcal.g.mol-1 e 4,89 Kcal.g.mol-1, respectivamente, para as amostras do suco de abacaxi natural e despectinizado, aumentando com o tratamento enzimático. A atividade enzimática proporcionou uma redução nos valores dos parâmetros de comportamento reológico das amostras, bem como na viscosidade aparente, em todas as temperaturas utilizadas, sendo o maior percentual de redução observado a 65 °C (41,66%.

  11. 橡胶籽提取物对新菠萝灰粉蚧的忌避作用%Pests and Diseases of Ananas comosus(L.)Merr in China and Their Control

    Institute of Scientific and Technical Information of China (English)

    张妮; 陈泽坦; 王发明

    2010-01-01

    就不同浓度的橡胶籽提取物对新菠萝灰粉蚧的选择性和非选择性忌避作用进行试验测定.选择性忌避试验结果表明:橡胶籽提取物300倍液(0.667ml/L)比其他浓度的忌避效果好,达到84.3%.非选择性忌避试验结果表明:以功夫3000倍液(0.008ml/L)作对照时,对新菠萝灰粉蚧的忌避率在900倍液(0.222ml/L)效果较好.

  12. Ripening-dependent metabolic changes in the volatiles of pineapple (Ananas comosus (L.) Merr.) fruit: I. Characterization of pineapple aroma compounds by comprehensive two-dimensional gas chromatography-mass spectrometry.

    Science.gov (United States)

    Steingass, Christof Björn; Carle, Reinhold; Schmarr, Hans-Georg

    2015-03-01

    Qualitative ripening-dependent changes of pineapple volatiles were studied via headspace solid-phase microextraction and analyzed by comprehensive two-dimensional gas chromatography quadrupole mass spectrometry (HS-SPME-GC×GC-qMS). Early green-ripe stage, post-harvest ripened, and green-ripe fruits at the end of their commercial shelf-life were compared to air-freighted pineapples harvested at full maturity. In total, more than 290 volatiles could be identified by mass spectrometry and their linear retention indices. The majority of compounds comprise esters (methyl and ethyl esters of saturated and unsaturated fatty acids, acetates), terpenes, alcohols, aldehydes, 2-ketones, free fatty acids, and miscellaneous γ- and δ-lactones. The structured separation space obtained by GC×GC allowed revealing various homologous series of compound classes as well as clustering of sesquiterpenes. Post-harvest ripening increased the diversity of the volatile profile compared to both early green-ripe maturity stages and on-plant ripened fruits.

  13. 菠萝冠芽变异及鸡冠状冠芽遗传稳定性初步研究%Variation and the Heredity Stability of Cockscomb Buds in Pineapple[Ananas comosus (L) Merr

    Institute of Scientific and Technical Information of China (English)

    吴青松; 孙伟生; 李运合; 孙光明

    2013-01-01

    The variation and the heredity stability of cockscomb buds for 3 pineapple cultivars, Smooth Cayenne, Comte de Paris, and Zhenzhu, were investigated The results showed that there were crown variants existed in Comte de Paris and Zhenzhu, but not in Smooth Cayenne. The crown variation percentage for Zhenzhu was significantly higher than that for Smooth Cayenne and Comte de Paris, mainly due to the significantly higher multiple crowns percentage. However, the cockscomb crown percentage was not different among the 3 pineapple cultivars in this study. Three cockscomb crown variants found from Comte de Paris and Zhenzhu were collected to propagate for investigation on crown style in offspring. All plants propagated from cockscomb crown resulted in a single crown on mature stage, indicating that cockscomb crown was influenced by environment and not heredity stability to offspring. However, the reappearance of multiple crown and cockscomb crown in the plant raised from the sucker buds of Zhenzhu cockscomb crown variants further demonstrated the Zhenzhu sucker planting material was easier to produce crown variation.%考察了无刺卡因、巴厘和珍珠3个菠萝品种吸芽苗冠芽的变异情况,结果显示:无刺卡因冠芽全为单冠芽,巴厘和珍珠则存在冠芽变异,其中珍珠发生冠芽变异显著高于无刺卡因和巴厘,主要是因为珍珠的复冠率显著高于无刺卡因和巴厘,但3个品种鸡冠状冠芽发生率的差异未达到显著.利用从巴厘和珍珠中发现的3株鸡冠状冠芽及其母株再次繁育成苗,所有鸡冠状冠芽苗成熟时均未再现鸡冠状冠芽表型,表明鸡冠状冠芽不能稳定遗传,受环境影响.然而珍珠鸡冠状冠芽母株的吸芽苗却又再现复冠和鸡冠状冠芽.再次表明品种珍珠的吸芽易于产生冠芽变异.

  14. Temperature and pH conditions for maximum activity of bromelain extracted from pineapple (Ananas Comosus L. Merril - doi: 10.4025/actascitechnol.v33i2.7928

    Directory of Open Access Journals (Sweden)

    Moacyr Jorge Elias

    2011-04-01

    Full Text Available Bromelain, the enzyme found in pineapple, hydrolyzes proteins’ peptide bonds, has several applications in the food industry and in medicine. Temperature and pH conditions for highest activity with casein as substrate were investigated from the bromelain recovered from industrialized pineapple (‘pérola’ variety fruit residues. Extract was obtained by grinding fruit rind and its internal stem. The activity was expressed in mmol tyrosine L-1 min. -1 by absorbance at 280 nm of aromatic amino acids produced in casein hydrolysis. Assays were undertaken in duplicate for two enzyme/substrate with ratios: 1/25 and 1/125 (mass. Eight-pointed experimental design was undertaken with central value for pH at 7.0 and for temperature at 35°C. Experimental results were processed by MINITAB 15 (Minitab Inc software which provided model’s equations and surface responses. Equations were mathematically processed by differential calculus producing graphs which exhibit the best activities according to temperatures. Loss of enzyme activity was reported with temperature increase at 1/25 ratio, due to a higher free enzyme, when compared to 1/125 ratio.

  15. Study on the accumulation of dry matter and NPK in Pineapple(Ananas comosus cv.Comte de Paris)plantlet%巴厘菠萝干物质和NPK养分累积规律研究

    Institute of Scientific and Technical Information of China (English)

    陈菁; 孙光明; 臧小平; 陆新华; 刘胜辉

    2010-01-01

    在8月中旬种植大小基本一致的巴厘菠萝小苗,然后在其生长发育的11个时间段测定各器官生物量和NPK养分含量.结果表明:植株干物质和NPK养分累积可分为4个阶段.第一阶段为缓慢累积阶段;第二阶段为快速累积阶段,此阶段干物质和NPK养分累积量分别占收获时累积量的39.6%、50.8%、45.8%、54.6%:第三阶段为累积最快阶段,此阶段干物质和NPK养分累积量分别占收获时累积量的19.5%、20.2%、17.8%、12_3%;第四阶段为缓慢累积阶段.此阶段干物质、P素还有一个显著累积,但N、K累积量已很少.2、叶片、茎、根、果柄干物质和NPK养分累积量在见红期以前是随着菠萝果实生长而逐步增加,在果实发育中后期逐步下降,而果实和芽十物质和NPK养分累积量从果实发育至收获是逐步增加的.

  16. Obtención de Preparados Enzimáticos a Partir de Tallos de Piña (Ananas Comosus con Potencialidades de uso en la Biotecnología y la Medicina.

    Directory of Open Access Journals (Sweden)

    Martha Hernández

    2005-01-01

    Full Text Available El aislamiento y purificación de productos naturales a partir de plantas es una vertiente muy importante de la biotecnología moderna. La bromelina (EC 3.4.22.32 es una proteasa aislada de plantas de piña que se ha empleado tradicionalmente en la industria alimenticia. La enzima también se ha usado en la industria farmacéutica. En la actualidad existe un renovado interés por ella, desde que fue descrita su actividad antitumoral y antimetastizante frente a algunos tumores. En este estudio, se obtuvo extracto crudo de bromelina a través de un procedimiento de extracción novedoso. Los rendimientos de la tecnología fueron de 20.8 g de producto/kg de tallos, con una actividad específica de 1.36 U/mg. Tiene pH óptimo 7 para la hidrólisis de hemoglobina durante 20 minutos. El producto aislado es muy estable en un rango de pH de 3-9 y temperaturas de hasta 50°C durante cuatro horas. El extracto crudo se utilizó en la industria biotecnológica para obtener un hidrolizado proteico de Clorella vulgaris, con un rendimiento (g de hidrolizado/100 g de biomasa de 30%. La preparación enzimática fue purificada. Se determinó la secuencia amino terminal de la mayor fracción obtenida. Se realizó un estudio toxicológico y farmacológico del producto parcialmente purificado por cromatografía de intercambio iónico en carboximetil celulosa-52 (CMC-52. La dosis letal media a dosis única fue 216.35 mg/kg y a dosis repetida 105.91 mg/kg. La proteasa, comparada con el citostático 5-Fluoracilo, mostró una marcada actividad antitumoral frente a los tumores trasplantables de ratón: leucemia P-388, carcinoma pulmonar de Lewis, tumor ascítico de Ehrlich, sarcoma-37 y adenocarcinoma mamario-755. Frente a este último se demostró por primera vez la actividad antitumoral de la bromelina.

  17. A New ELISA Using the ANANAS Technology Showing High Sensitivity to diagnose the Bovine Rhinotracheitis from Individual Sera to Pooled Milk.

    Science.gov (United States)

    Casarin, Elisabetta; Lucchese, Laura; Grazioli, Santina; Facchin, Sonia; Realdon, Nicola; Brocchi, Emiliana; Morpurgo, Margherita; Nardelli, Stefano

    2016-01-01

    Diagnostic tests for veterinary surveillance programs should be efficient, easy to use and, possibly, economical. In this context, classic Enzyme linked ImmunoSorbent Assay (ELISA) remains the most common analytical platform employed for serological analyses. The analysis of pooled samples instead of individual ones is a common procedure that permits to certify, with one single test, entire herds as "disease-free". However, diagnostic tests for pooled samples need to be particularly sensitive, especially when the levels of disease markers are low, as in the case of anti-BoHV1 antibodies in milk as markers of Infectious Bovine Rhinotracheitis (IBR) disease. The avidin-nucleic-acid-nanoassembly (ANANAS) is a novel kind of signal amplification platform for immunodiagnostics based on colloidal poly-avidin nanoparticles that, using model analytes, was shown to strongly increase ELISA test performance as compared to monomeric avidin. Here, for the first time, we applied the ANANAS reagent integration in a real diagnostic context. The monoclonal 1G10 anti-bovine IgG1 antibody was biotinylated and integrated with the ANANAS reagents for indirect IBR diagnosis from pooled milk mimicking tank samples from herds with IBR prevalence between 1 to 8%. The sensitivity and specificity of the ANANAS integrated method was compared to that of a classic test based on the same 1G10 antibody directly linked to horseradish peroxidase, and a commercial IDEXX kit recently introduced in the market. ANANAS integration increased by 5-fold the sensitivity of the 1G10 mAb-based conventional ELISA without loosing specificity. When compared to the commercial kit, the 1G10-ANANAS integrated method was capable to detect the presence of anti-BHV1 antibodies from bulk milk of gE antibody positive animals with 2-fold higher sensitivity and similar specificity. The results demonstrate the potentials of this new amplification technology, which permits improving current classic ELISA sensitivity limits

  18. A New ELISA Using the ANANAS Technology Showing High Sensitivity to diagnose the Bovine Rhinotracheitis from Individual Sera to Pooled Milk.

    Directory of Open Access Journals (Sweden)

    Elisabetta Casarin

    Full Text Available Diagnostic tests for veterinary surveillance programs should be efficient, easy to use and, possibly, economical. In this context, classic Enzyme linked ImmunoSorbent Assay (ELISA remains the most common analytical platform employed for serological analyses. The analysis of pooled samples instead of individual ones is a common procedure that permits to certify, with one single test, entire herds as "disease-free". However, diagnostic tests for pooled samples need to be particularly sensitive, especially when the levels of disease markers are low, as in the case of anti-BoHV1 antibodies in milk as markers of Infectious Bovine Rhinotracheitis (IBR disease. The avidin-nucleic-acid-nanoassembly (ANANAS is a novel kind of signal amplification platform for immunodiagnostics based on colloidal poly-avidin nanoparticles that, using model analytes, was shown to strongly increase ELISA test performance as compared to monomeric avidin. Here, for the first time, we applied the ANANAS reagent integration in a real diagnostic context. The monoclonal 1G10 anti-bovine IgG1 antibody was biotinylated and integrated with the ANANAS reagents for indirect IBR diagnosis from pooled milk mimicking tank samples from herds with IBR prevalence between 1 to 8%. The sensitivity and specificity of the ANANAS integrated method was compared to that of a classic test based on the same 1G10 antibody directly linked to horseradish peroxidase, and a commercial IDEXX kit recently introduced in the market. ANANAS integration increased by 5-fold the sensitivity of the 1G10 mAb-based conventional ELISA without loosing specificity. When compared to the commercial kit, the 1G10-ANANAS integrated method was capable to detect the presence of anti-BHV1 antibodies from bulk milk of gE antibody positive animals with 2-fold higher sensitivity and similar specificity. The results demonstrate the potentials of this new amplification technology, which permits improving current classic ELISA

  19. Ameliorative effect of Ananus comosus peel on 7, 12 dimethylbenz(α)anthracene induced mammary carcinogenesis with reference to oxidative stresss

    Institute of Scientific and Technical Information of China (English)

    M Kalaiselvi; D Gomathi; G Ravikumar; K Devaki; C Uma

    2013-01-01

    Objective: To Ananus comosus (A. comosus), has demonstrated a wide variety of biological activities which make it a good plant source for the treatment of many oxidative stress mediated diseases. The present study was aimed to evaluate therapeutic potential by assaying the activities of oxidative stress parameters in 7, 12 dimethylbenz(α)anthracene induced breast cancer rats. Methods: Animals were divided into five groups of six animals. Group I served as control, group II induced mammary carcinogenesis by 7, 12 dimethylbenz(α)anthracene, group III and IV are treatment groups (plant extract 250 mg/kg body weight and standard drug 10 mg/kg body weight) and group V served as animals treated with plant extract alone. All the animals were sacrificed after 30 days treatment and breast tissues are used for the analysis of protein content, enzymic and non-enzymic antioxidants using standard protocols. Results: The oral administrations of ethanolic peel extract of A. comosus (250 mg/kg body weight) to breast cancer bearing rats for 30 days demonstrated a significant (P<0.05) increased in body weight, tissue weight, protein content, enzymatic and non enzymatic levels. The altered activities of lipid peroxidation and tumor weight in breast tissue of control and experimental cancer bearing rats were significantly (P<0.05) were reverted to near normal levels by the administration of ethanolic extract suggesting that the extract have quenching capacity against free radicals thereby exhibiting anticancer potential of A. comosus in breast cancer bearing rats. Conclusions: Thus, modulatory effects of A. comosus on attenuating the lipid peroxidation and upregulation of oxidative stress key enzymes like enzymatic and non-enzymatic antioxidants, protein content afford a pledge for widespread use for the treatment of breast cancer in the future.

  20. A taste of pineapple evolution through genome sequencing.

    Science.gov (United States)

    Xu, Qing; Liu, Zhong-Jian

    2015-12-01

    The genome sequence assembly of the highly heterozygous Ananas comosus and its varieties is an impressive technical achievement. The sequence opens the door to a greater understanding of pineapple morphology and evolution.

  1. 7 CFR 319.56-47 - Certain fruits from Thailand.

    Science.gov (United States)

    2010-01-01

    ... indica), mangosteen (Garcinia mangoestana L.), pineapple (Ananas comosus), and rambutan (Nephelium...) Growing conditions. Litchi, longan, mango, mangosteen, pineapple, and rambutan must be grown in a... Thailand. (b) Treatment. Litchi, longan, mango, mangosteen, pineapple, and rambutan must be treated...

  2. 7 CFR 319.56-13 - Fruits and vegetables allowed importation subject to specified conditions.

    Science.gov (United States)

    2010-01-01

    ...)(2)(vi). Argentina Pineapple Ananas comosus Fruit (b)(2)(vi). Australia (Tasmania only) Pineapple... certification and reexamination at the port of departure prior to exportation. (vi) Only the Tahiti...

  3. CLONIG AND CHARACTERIZATION OF A NEW ICE NUCLEATION ACTIVEGENE FROM ICE NUCLEATION ACTIVE BACTERIUM ERWINIA ANANAS 110%冰核细菌(Erwinia ananas 110)冰核基因克隆和序列分析

    Institute of Scientific and Technical Information of China (English)

    唐朝荣; 孙福在; 赵廷昌; 李瑞峰

    2002-01-01

    从作者自行分离的冰核细菌(Erwinia ananas110)中克隆到我国第1个细菌冰核基因,并完成其序列测定和分析.所克隆基因编码区全长3921bp,编码1306aa,氨基酸序列明显分为3个区即N-端(161aa)、C-端(41aa)的单一序列区和中部的高度重复序列R区(1104aa),以16氨基酸为重复单元的R区占整个编码序列的84.5%.序列分析表明我们所克隆的基因为一个新冰核基因,将其命名为iceA,该基因已在GenBank上登录,登录号为:AF387802.

  4. Efeitos de herbicidas na atividade fotossintética e no crescimento de abacaxi (Ananas comossus Effects of herbicides on the photosynthetic activity of pineapple (Ananas comossus

    Directory of Open Access Journals (Sweden)

    M.G. Catunda

    2005-03-01

    Full Text Available O manejo de plantas daninhas na cultura do abacaxi é uma prática indispensável, sendo o controle químico cada vez mais utilizado, uma vez que permite o controle eficiente e não danifica as raízes da cultura. Todavia, há poucos trabalhos sobre interferência de herbicidas no crescimento da cultura; portanto, visando avaliar os efeitos de amicarbazone e diuron + paraquat, estes foram aplicados diretamente sobre as plantas, sendo os efeitos mensurados através da taxa de fluorescência da clorofila a e dos teores de clorofila a, clorofila b e carotenóides, além da avaliação de altura e biomassa seca de parte aérea (Wpa. O herbicida amicarbazone promoveu declínio nos teores de pigmentos fotossintéticos, não se verificando a mesma interferência para as variáveis altura e Wpa; no entanto, o herbicida diuron + paraquat foi letal para a cultura, haja vista a redução nos teores de clorofilas a e b e carotenóides, além da impossibilidade da medição da altura e Wpa (aos 120 DAT em função da morte das plantas aos 30 DAT, o que demonstra certa seletividade do amicarbazone à cultura do abacaxi.Weed management in pineapple crop is an indispensable practice, with chemical control being increasingly used since it allows efficient control without injury to the roots. Since little literature is available on herbicide interference in crop growth, the aim of this work was to evaluate the effects of the herbicides amicarbazone and diuron + paraquat applied directly on the plants. The herbicide effects were measured by the fluorescence rate of chlorophyll a and chlorophyll a, chlorophyll b and carotenoid contents, and by evaluating the height and dry biomass of the aerial part (Wpa. The application of amicarbazone reduced the photosynthetic pigment contents although the same interference in height and Wpa variables was not observed; however, diuron + paraquat herbicide caused the death of the pineapple plants, indicating a reduction in

  5. Produção de painéis de cimento Portland reforçados com fibra de curauá (Ananas erectifolius)

    OpenAIRE

    Bilcati, Géssica Katalyne

    2015-01-01

    O presente trabalho trata do estudo referente ao desempenho da fibra vegetal amazônica curauá (Ananas erectifolius) aplicada no processo de produção dos painéis de cimento portland, analisando a compatibilidade, os tratamentos realizados nas fibras de curauá, visando à melhoria das propriedades físicas e mecânicas dos painéis. A metodologia deste trabalho foi desenvolvida a partir da produção dos painéis cimento portland reforçados com fibras de curauá, variando os teores e os tipos de tratam...

  6. Extração e caracterização de uma enzima proteolítica do curauá (Ananas Erectifolius

    Directory of Open Access Journals (Sweden)

    Juliana Ferrari Ferreira

    2010-01-01

    Full Text Available Ananas erectifolius (curauá is a fibrous vegetable that can be found in North and Central West regions of Brazil. It is a bromeliaceae family plant which physico-chemical features provides great potential in the automobilistic industry as a source of fibers. As commonly described in other bromeliaceae species, it contains significant levels of bromelain, of high commercial value and wide range of aplications in food, pharmaceutical and cosmetics industry. In this paper, experimental tests were performed to evaluate the extraction of the proteolytic enzymes of the Ananas erectifolius under different pH and temperature conditions to determine in which ones the enzymatic activity would be the maximum for later purification of the bromelain. The two commercially available curauá varieties (white and purple were used in the experiments and the results showed the same optimal pH of 8.5 for both varieties and different temperatures of 30°C for the white one, and 10°C, 20°C and 35°C for the purple one.

  7. Prokaryotic expression of iceA gene from ice nucleation active bacteria Erwinia ananas 110 and analysis of ice nucleation activity%冰核细菌Erwinia ananas 110冰核基因iceA的原核表达及冰核活性分析

    Institute of Scientific and Technical Information of China (English)

    姚润贤; 袁哲明

    2013-01-01

    To obtain recombinant strain with high ice nucleation activity,iceA gene were amplified by PCR from ice nucleation active bacteria Erwinia ananas 110 and cloned into vector pMD19-T which was transformed into E.coli DH5α.The recombinant clones were screened by single and double digestion before sequenced.From the positive recombinant strain,iceA gene was subcloned into prokaryotic expression vector pET-23a(+),resulting in recombinant plasmid pET-23a(+)-ice which was transformed into E.coli BL21(DE3)pLysS and induced by IPTG.SDS-PAGE indicated that ice nucleation active protein was expressed as inclusion bodies with molecular weight of about 180 000.Ice nucleation activity test showed there was no difference in ice nucleation activity under-5,4,-3,and-2 ℃ between recombinant E.coli BL21(DE3)pLysS and wild ice nucleation active bacteria Erwinia ananas 110.%为获得具有高冰核活性的基因工程菌,从冰核细菌Erwinia ananas 110扩增冰核基因iceA,将其克隆到pMD 19-T载体上,转化大肠杆菌DH5α,单、双酶切鉴定并测序;阳性克隆目的片段亚克隆到表达载体pET-23a(+)上,转化大肠杆菌DH5αt,单、双酶切鉴定重组质粒;阳性重组质粒转化大肠杆菌BL21(DE3)pLysS,并经IPTG诱导表达.SDS-PAGE电泳检测表明,冰核基因iceA能够并以包涵体形式表达,相对分子质量约为180 000.冰核活性测定结果表明,重组菌BL21 (DE3)pLysS/pET-ice的冰核活性与野生冰核细菌Erwinia ananas 110在-5、-4、-3、-2℃下无明显差别.

  8. WILD PINEAPPLE (ANANAS BRACTEATUS (LINDL.), VAR. ALBUS) HARVESTED IN FOREST PATCHES IN RURAL AREA OF VIÇOSA, MINAS GERIAS, BRAZIL: EXCELLENT SOURCE OF MINERALS AND GOOD SOURCE OF PROTEINS AND VITAMIN C

    OpenAIRE

    GALDINO XAVIER DE PAULA FILHO; TIBÉRIO FONTENELLE BARREIRA; GILBERTO BERNARDO DE FREITAS; HÉRCIA STAMPINI DUARTE MARTINO; HELENA MARIA PINHEIRO SANT’ANA

    2016-01-01

    ABSTRACT In the rural region of the municipality of Viçosa, Minas Gerais, forest patches are encountered and they present a great diversity of wild and edible fruit, where wild pineapple (Ananas bracteatus (Lindl.), var. albus) is one of those of greatest occurrence. Given that, little is known about the nutritional characteristics of this fruit, the present study aimed to investigate the physical and physic-chemical characteristics, chemical composition (titratable acidity, total soluble so...

  9. Analysis of the Nutritional Components of Stichopus variegates Thelenota ananas and Stichopus Chloronotus Boandt%花刺参、梅花参和绿刺参营养成分分析

    Institute of Scientific and Technical Information of China (English)

    王远红; 于明明; 王冬燕; 郭丽萍; 王聪; 吕志华

    2010-01-01

    @@ 花刺参(Stichopus variegates)又称梅花小参、梅花参(Thelenota ananas)和绿刺参(Stichopus Chloronotus Boandt)又称四方刺参或方柱参,属于无脊椎动物棘皮动物门(Echinodermata)、海参纲(Holothuroidea)、楯手目(Aspidochirotida)、刺参科(Stichopodidae).

  10. Concentrações de ANA e BAP na micropropagação de abacaxizeiro L. Merrill (Ananas comosus e no cultivo hidropônico das plântulas obtidas in vitro The effect of ANA and BAP concentrations on the micropropagation and hydroponic cultures of pineapple

    Directory of Open Access Journals (Sweden)

    Cristiane Elizabeth Costa de Macêdo

    2003-12-01

    Full Text Available O efeito de diferentes concentrações de ANA e BAP na micropropagação do abacaxizeiro, bem como no cultivo em hidroponia das plântulas obtidas in vitro, foram estudados em brotos de abacaxizeiro da variedade Pérola, inoculados em meio de cultura básico MS, suplementado com os fitorreguladores BAP e ANA em diferentes concentrações. Caracteres morfológicos quantitativos relacionados ao crescimento dos brotos e das plântulas de abacaxizeiro foram avaliados respectivamente durante os cultivos in vitro e em hidroponia e mostraram que, o tratamento T1 (BAP = 1,0 mg L-1e ANA = 0,5 mg L-1 proporcionou a maior taxa média de regeneração de brotos e conseqüentemente uma maior produção de matéria fresca. Entretanto, a altura dos brotos e a formação de suas raízes foram maiores nos tratamentos T2 (BAP = 0,5 mg L-1 e ANA = 0,25 mg L-1 e T3 (BAP = 0,25 mg L-1 e ANA = 0,12 mg L-1 . Após sessenta dias de cultivo em hidroponia, todas as plântulas oriundas do tratamento T1 apresentaram um bom desenvolvimento, expresso pela maioria dos caracteres morfológicos avaliados. O sistema de micropropagação utilizado neste trabalho possibilitou a obtenção de brotos de abacaxizeiro Pérola, em quantidade suficiente e ao mesmo tempo de fácil individualização, seguida da regeneração de plântulas que foram cultivadas em hidroponia.The effect of different ANA and BAP concentrations on in vitro and hydroponic cultures were studied. Pineapple shoots derived from Pérola explants variety were inoculated in MS media containing BAP and ANA in different concentrations. Growth parameters of shoots and plantlets were measured for in vitro and hydroponic cultures. Showed a highest multiplication rates of shoots and consequently highest fresh matter production were obtained with BAP and ANA at the concentrations of 1,0 and 0,5 mg L-1 respectively. However, the shoot length as well as the root number formed were higher in the T2 (0,5 de BAP + 0,25 de ANA and T3 (0,25 de BAP + 0,12 de ANA treatments. The results showed that after sixty days of hydroponic culture and in the presence of T1 treatment, all plantlets had good developing that was observed in the majority of morphological growth parameters evaluated. The results showed that it is possible to obtain high quantities of shoots and then plantlets which could be cultivated in hydroponic culture using the pineapple micropropagation procedures methods.

  11. Changes of Endogenous Hormone Levels during the Inflorescence Differentiation in Pineapple (Smooth Cayenne. cv)%乙烯利诱导菠萝[Ananas comosus(L.)Merril]花芽分化过程与内源激素的关系

    Institute of Scientific and Technical Information of China (English)

    刘胜辉; 臧小平; 张秀梅; 孙光明

    2010-01-01

    以14月龄的无刺卡因品种为试材,研究乙烯利诱导菠萝花芽分化过程中茎尖及D叶中内源激素含量的动态变化.结果表明:乙烯利处理促进了菠萝植株内源C2H4和ABA的生成,同时降低了GA3、IAA及ZT水平,促成了菠萝花芽分化,但是乙烯利处理并不影响进入小花分化期后C2H4的释放;花芽开始分化后,C2H4和ABA水平下降,GA3、IAA、ZT水平升高,说明高水平的C2H4和ABA及较低水平的GA3、IAA、ZT有利于菠萝的成花,而较高的GA3、IAA、ZT及低水平的C2H4、ABA有利于菠萝小花的分化和发育.

  12. Authentication of pineapple (Ananas comosus [L.] Merr.) fruit maturity stages by quantitative analysis of γ- and δ-lactones using headspace solid-phase microextraction and chirospecific gas chromatography-selected ion monitoring mass spectrometry (HS-SPME-GC-SIM-MS).

    Science.gov (United States)

    Steingass, Christof B; Langen, Johannes; Carle, Reinhold; Schmarr, Hans-Georg

    2015-02-01

    Headspace solid phase microextraction and chirospecific gas chromatography-mass spectrometry in selected ion monitoring mode (HS-SPME-GC-SIM-MS) allowed quantitative determination of δ-lactones (δ-C8, δ-C10) and γ-lactones (γ-C6, γ-C8, γ-C10). A stable isotope dilution assay (SIDA) with d7-γ-decalactone as internal standard was used for quantitative analysis of pineapple lactones that was performed at three progressing post-harvest stages of fully ripe air-freighted and green-ripe sea-freighted fruits, covering the relevant shelf-life of the fruits. Fresh pineapples harvested at full maturity were characterised by γ-C6 of high enantiomeric purity remaining stable during the whole post-harvest period. In contrast, the enantiomeric purity of γ-C6 significantly decreased during post-harvest storage of sea-freighted pineapples. The biogenetical background and the potential of chirospecific analysis of lactones for authentication and quality evaluation of fresh pineapple fruits are discussed.

  13. Dicty_cDB: SHE773 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available te sequence. 52 0.034 1 CO731046 |CO731046.1 JBW004G03.b_019.abi Pineapple green mature fruit cDNA library A...nanas comosus cDNA clone JBW004G03 similar to unknown protein, mRNA sequence. 50 0.13 1 CO730778 |CO730778.1 JBW001D09.b_073.abi Pine...apple green mature fruit cDNA library Ananas comosus cDN

  14. AcEST: DK946990 [AcEST

    Lifescience Database Archive (English)

    Full Text Available tr|Q8H290|Q8H290_ANACO Tetrameric ubiquitin OS=Ananas comosus PE... 117 2e-25 tr|Q6UA12|Q6UA12_GOSBA Fibe...EDGRTLADYNIQKESTLHLVLRLRGG 228 >tr|Q6UA12|Q6UA12_GOSBA Fiber polyubiquitin OS=Gos

  15. AcEST: DK943822 [AcEST

    Lifescience Database Archive (English)

    Full Text Available tr|Q8H290|Q8H290_ANACO Tetrameric ubiquitin OS=Ananas comosus PE... 110 3e-23 tr|Q6UA12|Q6UA12_GOSBA Fiber ...TLADYNIQKESTLHLVLRLRGG 228 >tr|Q6UA12|Q6UA12_GOSBA Fiber polyubiquitin OS=Gossypium barbadense PE=2 SV=1 Len

  16. Dicty_cDB: SHB712 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 52 0.030 1 CO731046 |CO731046.1 JBW004G03.b_019.abi Pineapple green mature fruit ...78 |CO730778.1 JBW001D09.b_073.abi Pineapple green mature fruit cDNA library Ananas comosus cDNA clone JBW00

  17. 21 CFR 146.185 - Pineapple juice.

    Science.gov (United States)

    2010-04-01

    ..., ripe pineapple (Ananas comosus L. Merrill). The juice may have been concentrated and later... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Pineapple juice. 146.185 Section 146.185 Food and....185 Pineapple juice. (a) Identity. (1) Pineapple juice is the juice, intended for direct...

  18. Dicty_cDB: SHF821 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available cloneUI-D-GC1-aau-c-07-0-UI 3', mRNA sequence. 30 0.007 3 DT338982 |DT338982.1 JBW092C02.b_012.abi Pineapp...le week 5-10 nematode-infected gall cDNA library Ananas comosus cDNA clone JBW092C0

  19. Dicty_cDB: Contig-U16285-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 1_1( AF410881 |pid:none) Frankliniella occidentalis cystein... 178 4e-43 AF147207_1( AF147207 |pid:none) Art...8533 |pid:none) Ananas comosus mRNA for FB22 precursor... 178 5e-43 AF410883_1( AF410883 |pid:none) Franklin

  20. Dicty_cDB: SHL166 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available apple week 5-10 nematode-infected gall cDNA library Ananas comosus cDNA clone JBW092C02 similar to undisco...vered sequence, mRNA sequence. 30 0.004 3 AC141063 |AC141063.1 Homo sapiens chromos

  1. Dicty_cDB: SHB246 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available JBW092C02 similar to undiscovered sequence, mRNA sequence. 30 0.004 3 AC141063 |AC141063.1 Homo sapiens chr...8982.1 JBW092C02.b_012.abi Pineapple week 5-10 nematode-infected gall cDNA library Ananas comosus cDNA clone

  2. Droplet-vitrification and morphohistological studies of cryopreserved shoot tips of cultivated and wild pineapple genotypes

    Science.gov (United States)

    Germplasm conservation of pineapple [Ananas comosus (L.) Mer.] is crucial to preserve the genus’ genetic diversity to secure material for genetic improvement and to support innovative and new research. Long-term conservation is accomplished through cryopreservation that is done by storing cells or t...

  3. Using agronomic tools to improve pineapple quality and its uniformity in Benin

    NARCIS (Netherlands)

    Fassinou Hotegni, V.N.

    2014-01-01

    Keywords: Ananas comosus; Benin; cultural practices; fruit quality; hapas; heterogeneity; planting material; slips; suckers; supply chain; variation in quality; variation within crop; vigour. Poor average quality and uniformity in quality have become major issues in agri-food chains

  4. The pineapple genome and the evolution of CAM photosynthesis

    Science.gov (United States)

    Pineapple (Ananas comosus (L.) Merr.) is the most economically valuable crop possessing crassulacean acid metabolism (CAM), a photosynthetic carbon assimilation pathway with high water-use efficiency, and the second most important tropical fruit. We sequenced the genomes of pineapple varieties F153 ...

  5. Morfologia e viabilidade de grãos de pólen de acessos silvestres de abacaxi Morphology and viability of pollen grains from wild varieties of pineapple

    Directory of Open Access Journals (Sweden)

    Taliane Leila Soares

    2011-10-01

    Full Text Available O objetivo do trabalho foi avaliar a viabilidade de grãos de pólen de acessos silvestres de abacaxi mediante a percentagem de germinação in vitro, crescimento do tubo polínico em duas temperaturas de incubação e uso de carmim acético. Além disso, o trabalho busca reconhecer os padrões da morfologia polínica de algumas variedades de abacaxi. Foram utilizadas amostras de pólen coletadas de seis acessos de abacaxi: Silvestre-25, I-26/803 (Ananas macrodontes, Igor (A. comosus var. erectifolius, Ananás Santo Amaro, FRF-22 e FRF-32 (A. comosus var. bracteatus. Os grãos de pólen foram inoculados em meio de cultura contendo 10% de sacarose, 0,01% H3BO3, 0,01% KNO3, 0,03% Ca(NO32.4H2O, solidificado com 1% de ágar e pH ajustado para 6,5. Foram testadas as temperaturas de incubação de 25°C e 30°C. A viabilidade do pólen foi avaliada pela coloração com carmim acético a 2%. Todas as variedades de A. comosus apresentaram grãos de pólen de tamanho médio, simetria radial, âmbito ovalado a esferoidal, 2-porados, subprolatos, exina eutectada, reticulada, heterobrocada, lumens variando de arredondados a poligonais, sem granulações, com exceção dos dois acessos de A. macrodontes que apresentaram tamanho grande. Melhores taxas de germinação foram obtidas para A. macrodontes em comparação com o erectifolius e bracteatus. A maioria dos acessos apresentou viabilidade com carmim acético acima de 76%.The aim of this research was to evaluate the pollen grains viability from accessions of wild pineapple using in vitro pollen germination and the growth of the polinic tube in two different temperatures and acetic carmine. In addition, the study aimed to recognize the pattern of pollen morphology of some pineapple varieties. Samples from six pineapple's accessions: Silvestre-25, I-26/803 (Ananas macrodontes, Igor (A. comosus var. erectifolius, Ananás Santo Amaro, FRF-22 and FRF-32 (A. comosus var. bracteatus were used. The pollen grains

  6. AL Amyloidosis

    Directory of Open Access Journals (Sweden)

    Desport Estelle

    2012-08-01

    Full Text Available Abstract Definition of the disease AL amyloidosis results from extra-cellular deposition of fibril-forming monoclonal immunoglobulin (Ig light chains (LC (most commonly of lambda isotype usually secreted by a small plasma cell clone. Most patients have evidence of isolated monoclonal gammopathy or smoldering myeloma, and the occurrence of AL amyloidosis in patients with symptomatic multiple myeloma or other B-cell lymphoproliferative disorders is unusual. The key event in the development of AL amyloidosis is the change in the secondary or tertiary structure of an abnormal monoclonal LC, which results in instable conformation. This conformational change is responsible for abnormal folding of the LC, rich in β leaves, which assemble into monomers that stack together to form amyloid fibrils. Epidemiology AL amyloidosis is the most common type of systemic amyloidois in developed countries with an estimated incidence of 9 cases/million inhabitant/year. The average age of diagnosed patients is 65 years and less than 10% of patients are under 50. Clinical description The clinical presentation is protean, because of the wide number of tissues or organs that may be affected. The most common presenting symptoms are asthenia and dyspnoea, which are poorly specific and may account for delayed diagnosis. Renal manifestations are the most frequent, affecting two thirds of patients at presentation. They are characterized by heavy proteinuria, with nephrotic syndrome and impaired renal function in half of the patients. Heart involvement, which is present at diagnosis in more than 50% of patients, leading to restrictive cardiopathy, is the most serious complication and engages prognosis. Diagnostic methods The diagnosis relies on pathological examination of an involved site showing Congo red-positive amyloid deposits, with typical apple-green birefringence under polarized light, that stain positive with an anti-LC antibody by immunohistochemistry and

  7. WILD PINEAPPLE (ANANAS BRACTEATUS (LINDL., VAR. ALBUS HARVESTED IN FOREST PATCHES IN RURAL AREA OF VIÇOSA, MINAS GERIAS, BRAZIL: EXCELLENT SOURCE OF MINERALS AND GOOD SOURCE OF PROTEINS AND VITAMIN C

    Directory of Open Access Journals (Sweden)

    GALDINO XAVIER DE PAULA FILHO

    2016-01-01

    Full Text Available ABSTRACT In the rural region of the municipality of Viçosa, Minas Gerais, forest patches are encountered and they present a great diversity of wild and edible fruit, where wild pineapple (Ananas bracteatus (Lindl., var. albus is one of those of greatest occurrence. Given that, little is known about the nutritional characteristics of this fruit, the present study aimed to investigate the physical and physic-chemical characteristics, chemical composition (titratable acidity, total soluble solids, pH, moisture content, ash, protein, lipids and dietary fiber, carotenoids (a-carotene, ß-carotene, ß-cryptoxanthin and lycopene, vitamin C (AA and DHA by HPLC-DAD, vitamin E (a-, ß-, ?-, d-, tocopherols and tocotrienols by HPLC-fluorescence, and minerals (P, K, Ca, Mg, Cu, Fe, Zn, Mn, Na, Cr, Se and Mo by ICP-AES. Fruits showed a moisture content average of 78.5 g 100g-1, 16.3 ° Brix of soluble solids, titratable acidity equal to 1.71 g 100g-1 of citric acid, 1.66 g 100g-1 of dietary fiber, 1.41 g 100 g-1 of lipids and caloric density of 83.15 kcal 100g-1. We considered it a source of carbohydrates (12.82 g 100g-1, proteins (4.79 g 100g-1 and Zn (0.98 mg 100g-1; a good source of vitamin C (18.70 mg 100g-1; and an excellent source of Cu (0.48 mg 100g-1, Fe (2.05 mg 100g-1, Mn (8.87 mg 100g-1 and Mo (0.15 mg 100g-1.

  8. Dicty_cDB: SHJ605 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available .1 JBW092C02.b_012.abi Pineapple week 5-10 nematode-infected gall cDNA library Ananas comosus cDNA clone JBW...GC1 Alexandrium tamarense cDNA clone UI-D-GC1-aau-c-07-0-UI 3', mRNA sequence. 30 0.004 3 DT338982 |DT338982

  9. Dicty_cDB: SHJ827 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 2.1 JBW092C02.b_012.abi Pineapple week 5-10 nematode-infected gall cDNA library Ananas comosus cDNA clone JB...-GC1 Alexandrium tamarense cDNA clone UI-D-GC1-aau-c-07-0-UI 3', mRNA sequence. 30 0.003 3 DT338982 |DT33898

  10. Dicty_cDB: SHF788 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 1 Alexandrium tamarense cDNA cloneUI-D-GC1-aau-c-07-0-UI 3', mRNA sequence. 30 0.007 3 DT338982 |DT338982.1 JBW092C02.b_012.abi Pinea...pple week 5-10 nematode-infected gall cDNA library Ananas comosus cDNA clone JBW092

  11. Spider-fed bromeliads: seasonal and interspecific variation in plant performance

    OpenAIRE

    GONCALVES, Ana Zangirolame; MERCIER, Helenice; MAZZAFERA, Paulo; Romero,Gustavo Quevedo

    2011-01-01

    Background and Aims Several animals that live on bromeliads can contribute to plant nutrition through nitrogen provisioning (digestive mutualism). The bromeliad-living spider Psecas chapoda (Salticidae) inhabits and breeds on Bromelia balansae in regions of South America, but in specific regions can also appear on Ananas comosus (pineapple) plantations and Aechmea distichantha. Methods Using isotopic and physiological methods in greenhouse experiments, the role of labelled ((15)N) spider faec...

  12. Physico-Chemical Properties, Antioxidant Activity and Mineral Contents of Pineapple Genotypes Grown in China

    OpenAIRE

    Xin-Hua Lu; De-Quan Sun; Qing-Song Wu; Sheng-Hui Liu; Guang-Ming Sun

    2014-01-01

    The fruit physico-chemical properties, antioxidant activity and mineral contents of 26 pineapple [Ananas comosus (L.) Merr.] genotypes grown in China were measured. The results showed great quantitative differences in the composition of these pineapple genotypes. Sucrose was the dominant sugar in all 26 genotypes, while citric acid was the principal organic acid. Potassium, calcium and magnesium were the major mineral constituents. The ascorbic acid (AsA) content ranged from 5.08 to 33.57 mg/...

  13. 凤梨科植物的引种收集及栽培技巧

    Institute of Scientific and Technical Information of China (English)

    张淑红

    2007-01-01

    @@ 说到凤梨科植物,人们首先想到的是著名的热带水果--菠萝(Ananas comosus),菠萝是凤梨科植物里的一个种.凤梨科是单子叶植物的一个大科,全世界共有45个属2500多种.

  14. Viability of mycelial growth of Coprinus Comatus in culture medium based on organic residues

    OpenAIRE

    de Almeida, Lais Benes; Sales-Campos,Ceci; Melo de Carvalho, Cristiane Suely; de Almeida Minhoni, Marli Teixeira; Nogueira de Andrade, Meire Cristina

    2012-01-01

    The objective of this work was to evaluate the mycelial growth of the Coprinus comatus strain CCO 01/01 in culture based on organic residues of Saccharum officinarum (sugarcane bagasse), Citrus sinensis (orange bagasse), Ananas comosus (pineapple residues) and Musa sp. (banana leaf), supplemented with wheat bran in the proportions of 0, 10 and 20%, kept at 27 degrees C. The mycelial growth of C. comatus was evaluated daily by measurement of the diameter of the colony during seven days of incu...

  15. Using agronomic tools to improve pineapple quality and its uniformity in Benin

    OpenAIRE

    Fassinou Hotegni, V.N.

    2014-01-01

    Keywords: Ananas comosus; Benin; cultural practices; fruit quality; hapas; heterogeneity; planting material; slips; suckers; supply chain; variation in quality; variation within crop; vigour. Poor average quality and uniformity in quality have become major issues in agri-food chains. This is also the case in the pineapple sector in Benin where less than 2% of the fresh pineapple is exported to international markets. The average quality of pineapple delivered to other markets, local and region...

  16. Abu al-Layth al-Libi

    Science.gov (United States)

    2015-02-01

    He has described at length the various methods to which the prisoners were subjected, including sleep depravation, nudity and the use of electrodes...suspects of the Riyadh bombing acknowledge their involvement on television , he was under the impression that they too had been subjugated to this... sleeping , al-Libi, al-Zawi and `Abd al-Karim managed to escape by jumping from the six-meter-tall wall of al-Ruways.38 Their breakout led to a

  17. Controle fitopatológico do Fusarium subglutinans pelo óleo essencial do capim-citronela (Cymbopogon nardus L. e do composto citronelal Fungitoxic activity of essential oil of citronella grass (Cymbopogon nardus L. and compound citronellal

    Directory of Open Access Journals (Sweden)

    P.T.L. Seixas

    2011-01-01

    Full Text Available Este trabalho teve como objetivo avaliar o efeito fungitóxico do óleo essencial de capim-citronela e do seu constituinte majoritário citronelal sobre a inibição micelial do fitopatógeno Fusarium subglutinans, agente causal da fusariose da cultura do abacaxi (Ananas comosus. Para avaliar o efeito do óleo essencial no crescimento micelial do fungo, foram utilizadas seis alíquotas (0, 5, 10, 15, 20 e 25 ìL do óleo e do citronelal que foram distribuídas na superfície do meio BDA (batata-dextrose-ágar antes da repicagem do fungo. O crescimento micelial foi medido após 48 h de instalação do experimento e em cinco épocas de avaliação (2, 4, 6, 8 e 10 dias após repicagem. Os resultados indicaram que o óleo essencial do capim-citronela demonstrou maior efeito inibitório do crescimento micelial do fungo F. subglutinans do que o composto citronelal. Em todas as alíquotas utilizadas o óleo essencial proporcionou menor taxa de crescimento micelial do que o citronelal.This study aimed to evaluate the fungitoxic effect of the essential oil of citronella grass and its major constituent citronellal on the inhibition of mycelial pathogen Fusarium subglutinans, the causal agent of Fusarium culture of pineapple (Ananas comosus. To evaluate the effect of essential oil in the mycelial growth of the fungus were used six rates (0, 5, 10, 15, 20 and 25 mL of oil that were distributed on the surface of PDA medium (potato dextrose agar before subculturing of the fungus. Mycelial growth was measured after 48 h of the experiment and five times of assessment (2, 4, 6, 8 and 10 days after subculturing. The results indicated that the essential oil of citronella grass showed higher inhibitory effect of mycelial growth of the fungus F. subglutinans than compound citronellal. In all rates used of the essential oil gave lower growth rate than the mycelial citronellal.

  18. New species of Rhinoleucophenga, a potential predator of pineapple mealybugs Nova espécie de Rhinoleucophenga, potencial predadora da cochonilha-do-abacaxizeiro

    Directory of Open Access Journals (Sweden)

    Mark Paul Culik

    2009-04-01

    Full Text Available The objective of this work was to describe a new species of Rhinoleucophenga (Diptera: Drosophilidae. Rhinoleucophenga capixabensis sp. nov. is described based on specimens collected from pineapple (Ananas comosus var. comosus infested with Dysmicoccus brevipes (Hemiptera: Pseudococcidae in the state of Espírito Santo, Brazil. Distinguishing characters of R. capixabensis sp. nov. include hyaline wings, a strong seta on the second antennal segment, body length of about 2-3 mm, uniformly dark brown scutellum, one pair of strong prescutellar setae, ventral epandrial lobes with about 17-18 teeth, and female cerci with approximately 20 long setae.O objetivo deste trabalho foi descrever uma nova espécie de Rhinoleucophenga (Diptera: Drosophilidae. Rhinoleucophenga capixabensis sp. nov. foi descrita com base em espécimes coletados em associação com Dysmicoccus brevipes (Hemiptera: Pseudococcidae em Ananas comosus var. comosus, no Estado do Espírito Santo, Brasil. As principais características de R. capixabensis sp. nov. incluem asas hialinas, uma seta forte no segundo segmento da antena, comprimento do corpo de aproximadamente 2-3 mm, escutelo uniformemente castanho-escuro, um par robusto de setas prescutelares, lobos epadriais ventrais com aproximadamente 17-18 dentes e cercos da fêmea com aproximadamente 20 setas longas.

  19. Callus cell, shoot and stem proliferation data from pineapple crown and banana inflorescence in vitro: Biochemical and antioxidant properties

    Directory of Open Access Journals (Sweden)

    ABM Sharif Hossain

    2017-02-01

    Full Text Available The data article contains the experimental data and images on the callus cell, shoot and stem proliferation from pineapple crown slice and banana inflorescence in vitro. Investigated data are related to the research article “Effects of benzylaminopurine and naphthalene acetic acid on proliferation and shoot growth of pineapple (Ananas comosus L. Merr in vitro” Alsaif et al. (2011 [1] and “Plantlet Production through Development of Competent Multiple Meristem Cultures from Male Inflorescence of Banana, Musa acuminta cv. ׳Pisang Mas׳(AA” Wirakarnain et al. (2008 [2]. In the experimental data 1, physiological, (shoot weight, number length and stem proliferation biochemical (total sugar and chlorophyll and nutritional ((K+ and NO3− data using BAP, MS medium and NAA growth regulators in pineapple have been explored. In the experimental data 2, physiological, (callus weight, shoot number and length biochemical (total sugar, chlorophyll, total phenol, DPPH and nutritional (K+ and NO3− data employing BAP +IAA, MS medium and NAA growth regulators in banana have been exhibited. Overall quantitative measurement was observed by Spectrophotometer. In the experimental data, BAP was shown the best effective hormone for the both pineapple and banana explants regeneration.

  20. /Cu-Al System

    Science.gov (United States)

    Kish, Orel; Froumin, Natalya; Aizenshtein, Michael; Frage, Nachum

    2014-05-01

    Wettability and interfacial interaction of the Ta2O5/Cu-Al system were studied. Pure Cu does not wet the Ta2O5 substrate, and improved spreading is achieved when relatively a high fraction of the active element (~40 at.% Al) was added. The Al2O3 and AlTaO4 phases were observed at the Ta2O5/Cu-Al interface. A thermodynamic evaluation allowed us to suggest that the lack of wetting bellow 40 at.% Al is due to the presence of a native oxide, which covers the drop. The conditions of the native oxide decomposition and the formation of the volatile Al2O suboxide strongly depend on the vacuum level during sessile drop experiments and the composition of the Cu-Al alloy. In our case, Al contents greater than 40% provides thermodynamic conditions for the formation of Al2O (as a result of Al reaction with Al2O3) and the drop spreading. It was suggested that the final contact angle in the Ta2O5/Cu-Al system (50°) is determined by Ta adsorption on the newly formed alumina interlayer.

  1. Aussprache als motorische Fertigkeit

    DEFF Research Database (Denmark)

    Bonner, Maria

    2013-01-01

    Authentische Aussprache gilt im Fremdsprachenunterricht als schwer erreichbares Ziel, fremdsprachlicher Akzent wird als nahezu unvermeidbar gesehen. Weder die Hypothese einer kritischen Periode für den Erwerb einer authentischen Aussprache noch das Konzept der Fossilierung im Sprachlernprozess...

  2. Amyotrophic Lateral Sclerosis (ALS)

    Science.gov (United States)

    ... ALS Neurons' broken machinery piles up in ALS Esclerosis Lateral Amiotrófica Dormant viral genes may awaken to ... Dementia Information Page Multifocal Motor Neuropathy Information Page Multiple Sclerosis Information Page Muscular Dystrophy Information Page Myasthenia ...

  3. Volver al desarrollo

    Directory of Open Access Journals (Sweden)

    Jaime Ornelas Delgado

    2012-01-01

    Full Text Available El desarrollo, categoría empleada para expresar el crecimiento económico, surge durante la "guerra fría" y si bien fue propuesta por teóricos metropolitanos, se asumió en Latinoamérica como parte de los instrumentos disponibles para lograr el crecimiento y alternativa al socialismo. El desarrollo mantuvo su vigencia entre 1945 y 1975, cuando al advenimiento del neoliberalismo fue paulatinamente retirado de la agenda de las preocupaciones nacionales e internacionales. Al iniciarse el siglo xxi, el fracaso del mercado autorregulado trajo de nueva cuenta al debate los problemas del desarrollo, lo que obliga a revisarlo críticamente, tanto como al concepto mismo para mostrar su carácter colonial, si se quiere construir caminos ajenos al neoliberal y superar los problemas que han hecho de Latinoamérica una de las regiones más desiguales del mundo.

  4. Alles is al uitgevonden

    NARCIS (Netherlands)

    J.G.M. van Marrewijk (Charles)

    1996-01-01

    textabstractDe voordelen van vrijhandel zijn zeer beperkt, al-thans als we de empirische schattingen van vooraanstaande economen en internationale organisaties mogen geloven. Zo worden de voordelen van liberalisatie voor de Europese Unie geraamd op ongeveer 0,5% van het bnp 1. De door het IMF gescha

  5. Gezondheid als marketinginstrument

    NARCIS (Netherlands)

    Vijver, van de L.P.L.; Berg, van den I.

    2008-01-01

    Is biologisch voedsel beter dan gangbaar? Hoe hard zijn de bewijzen daarvoor? En hoe sterk moet het bewijs zijn voordat je het als claim op een product mag zetten? In dit Biokennis bericht een overzicht van de wetenschappelijke conclusies en de mogelijkheden om biologisch te promoten als beter, neer

  6. Initial Symptoms of ALS

    Science.gov (United States)

    ... Research In Your Community Advocate Get Involved Donate Symptoms and Diagnosis En español Symptoms The initial symptoms of ALS can be quite ... the eyes and bladder are generally not affected. Diagnosis ALS is a difficult disease to diagnose. There ...

  7. Volver al desarrollo

    OpenAIRE

    Jaime Ornelas Delgado

    2012-01-01

    El desarrollo, categoría empleada para expresar el crecimiento económico, surge durante la "guerra fría" y si bien fue propuesta por teóricos metropolitanos, se asumió en Latinoamérica como parte de los instrumentos disponibles para lograr el crecimiento y alternativa al socialismo. El desarrollo mantuvo su vigencia entre 1945 y 1975, cuando al advenimiento del neoliberalismo fue paulatinamente retirado de la agenda de las preocupaciones nacionales e internacionales. Al iniciarse el siglo xxi...

  8. Genomic analyses of the CAM plant pineapple.

    Science.gov (United States)

    Zhang, Jisen; Liu, Juan; Ming, Ray

    2014-07-01

    The innovation of crassulacean acid metabolism (CAM) photosynthesis in arid and/or low CO2 conditions is a remarkable case of adaptation in flowering plants. As the most important crop that utilizes CAM photosynthesis, the genetic and genomic resources of pineapple have been developed over many years. Genetic diversity studies using various types of DNA markers led to the reclassification of the two genera Ananas and Pseudananas and nine species into one genus Ananas and two species, A. comosus and A. macrodontes with five botanical varieties in A. comosus. Five genetic maps have been constructed using F1 or F2 populations, and high-density genetic maps generated by genotype sequencing are essential resources for sequencing and assembling the pineapple genome and for marker-assisted selection. There are abundant expression sequence tag resources but limited genomic sequences in pineapple. Genes involved in the CAM pathway has been analysed in several CAM plants but only a few of them are from pineapple. A reference genome of pineapple is being generated and will accelerate genetic and genomic research in this major CAM crop. This reference genome of pineapple provides the foundation for studying the origin and regulatory mechanism of CAM photosynthesis, and the opportunity to evaluate the classification of Ananas species and botanical cultivars.

  9. Tussentijds Toetsen als Leerstrategie

    NARCIS (Netherlands)

    Dirkx, Kim; Kester, Liesbeth; Kirschner, Paul A.

    2013-01-01

    Dirkx, K. J. H., Kester, L., & Kirschner, P. A. (2013, April). Tussentijds toetsen als Leerstrategie [Testing as Learning Strategy]. Presentation at the Miniconference on Assessment from the Mastersprogram Educational Sciences, Heerlen, The Netherlands.

  10. Analyzing Al Qaedaism

    Institute of Scientific and Technical Information of China (English)

    Yuan Hua

    2008-01-01

    Three major events are responsible for the rampancy today of Al Qaedaism, the doctrine of Al Qaeda, an Islam extremist organization. They are: the Anti-Soviet Afghanistan War for the birth of the group; the September 11 terrorist attacks for the spread of its extremist demagogy and the war on terror for its branches networking into alliances worldwide.Al Qaedaism aims at driving away Western forces on the Moslem lands, overthrowing local Pro-West regimes, destroying Israel and setting up a Caliphate empire. Its rampancy has resulted from a combination of diverse factors at work under specific historical conditions rather an inevitable outcome of the growth of the Islam religion.As an ideological weapon for launching Jihad to oust Western influence and topple local regimes, Al Qaedaism reflects an extremist portrayal of modern Islam society, politics, economy and culture.

  11. Lou Gehrig's Disease (ALS)

    Science.gov (United States)

    ... to speak and write. Hawking, who has a wife and three children, once said, "The prospect of ... and arm movement. With patience, the families of patients with ALS can learn to communicate effectively with ...

  12. Sociale media als leermiddel

    NARCIS (Netherlands)

    Rubens, Wilfred

    2012-01-01

    Rubens, W. (2012, 5 juni). Sociale media als leermiddel. Presentatie over het gebruik van sociale media binnen het onderwijs, verzorgd tijdens een ouderraadbijeenkomst van de Katholieke Scholengemeenschap Etten-Leur, Etten-Leur, Nederland.

  13. Amyotrophic lateral sclerosis (ALS)

    Science.gov (United States)

    Lou Gehrig disease; ALS; Upper and lower motor neuron disease; Motor neuron disease ... 98. Shaw PJ. Amyotrophic lateral sclerosis and other motor neuron diseases. In: Goldman L, Schafer AI, eds. Goldman's Cecil ...

  14. Advanced Light Source (ALS)

    Data.gov (United States)

    Federal Laboratory Consortium — The Advanced Light Source (ALS), a world leader in soft x-ray science, generates light in the wavelengths needed for examining the atomic and electronic structure of...

  15. Trauer als Biografiegenerator

    OpenAIRE

    Winkel, Heidemarie

    2008-01-01

    Der Tod eines Alter Ego erweist sich für Trauernde als gravierende Kontingenzerfahrung. Sie vermag das individuelle Selbstverständnis in umfassender Weise zu erschüttern, wobei sich Trauer als Schmerz von unvergleichlicher Intensität und Tiefe konstituiert. Die emotionale Erfahrung wird hierbei – nach Maßgabe psychologischer und therapeutischer Vorstellungen – zum Ausgangspunkt und zum Maßstab individueller Selbstvergewisserung. Diese Auffassung von Trauer korrespondiert mit einer allgemeinen...

  16. Der Preis als Kaufbarriere?

    DEFF Research Database (Denmark)

    Mueller Loose, Simone

    2012-01-01

    Die Preispolitik hat eine herausragende Stellung im Marketing. Der Preis eines Produktes ist für Verbraucher ein direkter Kostenfaktor und für Unternehmen die Stellgröße im Mar-ketingmix, die den Umsatz am stärksten beeinflusst (vgl. Nieschlag et al. 2002). Wenn die Akzeptanz und der regelmäßige...... Verzehr gesunder Lebensmittel erhöht werden soll, ist es sowohl für die Verbraucherpolitik als auch für Lebensmittelproduzenten wichtig, den Ein-fluss des Preises auf die Kaufentscheidung zu verstehen. Aus Verbrauchersicht ist der Preis neben dem Geschmack die wichtigste Produkteigen-schaft beim Kauf von...... Lebensmitteln (vgl. Brunsø/Grunert 1998) und stellt eine Kaufbarrie-re für Lebensmittel mit gesundheitsfördernden Eigenschaften dar. Studien in den USA (vgl. Cassady et al. 2007), Frankreich (vgl. Drewnowski et al. 2004; Andrieu et al. 2005) und Deutschland (vgl. Karg et al. 2008) kommen übereinstimmend zu dem...

  17. Leakage current behaviors of Al/ZrO2/Al and Al/YSZ/Al devices

    Science.gov (United States)

    Yeh, Tsung-Her; Lin, Ruei-De; Cherng, Bo-Ruei; Cherng, Jyh-Shiarn

    2015-01-01

    The leakage current behaviors of Al/ZrO2/Al and Al/yttria stabilized zirconia (YSZ)/Al devices are investigated for resistive random access memory (RRAM) applications. A silicon oxide layer (450 nm) is first formed on a Si wafer by thermal oxidation. Onto it an Al bottom electrode (270 nm), a ZrO2 or YSZ nano-film (75 nm), and an Al top electrode (270 nm) are sequentially deposited by sputtering. These RRAM devices exhibit ohmic behaviors in the low-field region, while Schottky and Poole-Frenkel emissions take over in the high-field regions. Both the Schottky and trap barrier levels are decreased when monoclinic ZrO2 is replaced by cubic YSZ in the metal/oxide/metal structure. This is attributed not only to the higher symmetry crystal structure and lower binding energy of YSZ, but also to the formation of more oxygen vacancies and their re-distribution associated with yttria doping.

  18. Cuidando al adulto y al anciano

    Directory of Open Access Journals (Sweden)

    Alba Lucero López Díaz

    2003-01-01

    Full Text Available El propósito del artículo es describir, desde el ámbito universitario, la experiencia en la aplicación de la Teoría del Déficit de Autocuidado (TDAC con la población adulta y anciana. Se explican los procesos y resultados desde la preparación docente, la conformación de la asignatura, hasta el desarrollo de la experiencia práctica en el ámbito hospitalario y de la vida cotidiana. La experiencia muestra la adecuación de la TDAC para abordar el cuidado de esta población y la necesidad de desarrollar otros estudios al respecto.

  19. Fortschritt als Fortschrittsdiskurs

    Directory of Open Access Journals (Sweden)

    Rainer Schmid-Zartner

    2014-12-01

    Full Text Available Zur Erzielung gesellschaftlichen Fortschritts in demokratisch verfassten Gesellschaften soll die Möglichkeit am Fortschrittsdiskurs teilnehmen zu können für alle Mitglieder der Gesellschaft als selbstverständliche Prämisse gelten. Fortschritt wird also als Bewusstseinsbildung dem Fortschritt selbst gegenüber und als Entscheidungs- und Handlungsfähigkeit auf individueller und gesellschaftlicher Ebene definiert. Dies setzt entsprechende Maßnahmen zur Befähigung dazu voraus, die alle Mitglieder der Gesellschaft erfassen. Dabei ist der Bildungsbereich angesprochen, insbesondere der Schulunterricht. Neben dem in der aktuellen blidungspolitischen Debatte forcierten Fokus auf den Aspekt der Kompetenzorientierung, der hier nicht infrage gestellt wird, ist als notwendige Ergänzung ein reflexionsorientierter Schulunterricht in allen Bildungsfächern einzufordern und umzusetzen. Im Weiteren beschreibt der Text den Weg von der Brauchbarkeit zur Bedeutsamkeit von schulischem Wissen durch Relevanzreflexionsprovokationen im Schulunterricht, um Sinn und Bedeutung der vermittelten Bildungsinhalte zu verhandeln. Am Beispiel von Mathematik als Bildungsfach wird der Inhalt von reflexionsorientertem Unterricht verdeutlicht. Darüber hinaus beschreibt der Text eine Reihe von konkreten Fallbeispielen aus der aktuell versuchsweise durchgeführten Unterrichtspraxis in Mathematik.

  20. Modification of residual stress in Al-[AlBO]w/Al compound plate

    Institute of Scientific and Technical Information of China (English)

    姜传海; 吴建生; 王德尊

    2001-01-01

    The application of compound parts of Al-[AlBO]w/Al not only reduces the cost of the parts but also improves its properties. However, there is a large thermal residual stress between Al and [AlBO]w/Al, and it is harmful for practical application. From the theoretical analyses and experimental results, it was found that by the compressive pre-plastic deformation perpendicular to the interface between Al and [AlBO]w/Al, the interlayer residual stress of compound parts can be reduced, while the mechanical properties of compound parts can be improved.

  1. Pornographie als Metapher

    Directory of Open Access Journals (Sweden)

    Caroline Schubarth

    2010-03-01

    Full Text Available In diesem Artikel werden unterschiedliche metaphorische Verwendungen des Pornografiebegriffs und deren Implikationen untersucht. Während die Existenz von Pornografie der feministischen Anti-Porno-Bewegung als Erklärung für die anhaltende Diskriminierung von Frauen in westlichen Gesellschaften dient, nutzen rechtskonservative Kräfte den Pornografievorwurf als Rechtfertigung für die Zensur von als deviant empfundenen Identitäten und sexuellen Praktiken.This article examines different metaphorical uses of the term pornography and the resulting implications. While the existence of pornography for the feminist anti-porno movement serves as factor in the continuing discrimination of women in western societies, conservative powers on the right use the allegation of pornography as a justification for censorship of those identities and sexual practices deemed deviant.

  2. Altern als Widerstand

    Directory of Open Access Journals (Sweden)

    Roberta Maierhofer

    2005-07-01

    Full Text Available Gullettes kulturwissenschaftliche Untersuchung Aged by Culture ist wie bereits ihre zwei vorangehenden Werke, die sich mit Altern beschäftigen – Safe at Last in the Middle Years: The Invention of the Midlife Progress Novel (1988 und Declining to Decline. Cultural Combat and the Politics of the Middle (1997 –, von großem persönlichen Engagement und durch ein politisches Anliegen motiviert. Sowohl die Dringlichkeit als auch der Widerstand, den Gullette, die sich als „age critic“ definiert, als moralische und politische Notwendigkeit postuliert, werden in der Zweiteilung der Abhandlung angesprochen: „Cultural Urgencies“ und „Theorizing Age Resistantly“. Während Gullette den Begriff „aged by culture“ bereits in Declining to Decline einführt, stellt sie ihn nun in den Mittelpunkt ihrer Untersuchung. Das Buch ist einerseits einer gesellschaftspolitischen Analyse der USA gewidmet, andererseits wird eine Theorie des Widerstands gegenüber Altersdiskriminierung entwickelt.

  3. Carta al Editor

    Directory of Open Access Journals (Sweden)

    José Andrés Burgos Zuleta

    2010-06-01

    Full Text Available La medicina al basarse generalmente en la clínica, correlacionaexámenes complementarios y organiza un diagnóstico y tratamiento, es una disciplina donde es imprescindible saber recolectar, analizar, interpretar y concretar una respuesta adecuada, es así que la información que se recibe es esencial para el desempeño médico.La certeza es una realidad que se da en algunas situaciones y enotras las probabilidades establecen conclusiones. Lo normal muchas veces se define sobre la base de criterios estadísticos, basados en riesgos poblacionales que no necesariamente se aplican al caso boliviano.

  4. Ongelijkheid als beleidsvraagstuk

    NARCIS (Netherlands)

    Wilterdink, N.

    2014-01-01

    Op het hoogtepunt van de hype rond het ongelijkheidsonder­zoek van Piketty presenteerde de WRR zijn verkenningHoe ongelijk is Nederland? Voor de onafhankelijke denktank is stelling nemen in een politiek vraagstuk als dat van ongelijkheid best ingewikkeld.

  5. MEMORE AL AMIKO

    Institute of Scientific and Technical Information of China (English)

    Osmo; BULLER

    1999-01-01

    Skuis min la novajo, ke mia amiko kajkolego Dai Song’en forlais nin por iam. Lakruela fatalo foriris lin, kiam li ankoraǚ estismeze de sia laboro, plena de planoj kaj tiel ju-ha. Kun muta senpovo ni devas subii al lasenkompata trudo de la sorto, kvankam la men-so ribelas kaj rifuzas kredi ke ipovas esti vero.

  6. Abraham Kuyper als theoloog

    Directory of Open Access Journals (Sweden)

    W. H. Velema

    1989-06-01

    Full Text Available Men zou in Nederland de herdenking van Kuypers honderdvijftigste geboortedag hebben kunnen aangrijpen als een gelegenheid om diens theologie en diens levenswerk in kerk en samenleving te evalueren. Niemand kan ontkennen dat er in genoemde publicaties iets van een evaluatie is te vinden.

  7. Kulturmorphologie als Zivilisationskritik

    DEFF Research Database (Denmark)

    Peters, Rikke Alberg

    2010-01-01

    Der Untergang des Abendlandes wird oft als dasjenige Werk betrachtet, das am tiefsten die national gefärbte Gegenüberstellung von Kultur und Zivilisation in der Zwischenkriegszeit geprägt und propagiert hat. Wie auch bei anderen Autoren der Konservativen Revolution, vor allem bei Thomas Mann und...

  8. Comentarios al Salto Social

    Directory of Open Access Journals (Sweden)

    Facultad de Ciencias Económicas

    1995-06-01

    Full Text Available 1. El Plan considera que la política social es un componente central de la estrategia macroeconómica. A diferencia de la administración Gaviria, lo social ha dejado de ser un asunto marginal, es consustancial al desarrollo y, por tanto, se le confiere una dimensión cualitativamente diferente.

  9. Calculation of interaction of AlCl, AlCl2 and AlCl3 on Al4C3 (001) Al4CO4 (001) and Al2CO (001) planes

    Institute of Scientific and Technical Information of China (English)

    段少飞; 陈秀敏; 杨斌; 郁青春; 徐宝强; 刘大春

    2015-01-01

    To make sure the intermediate products of the carbothermic reduction of Al2O3 process, such as Al4CO4, Al2CO and Al4C3, and the interaction of AlCl, AlCl2, AlCl3 with Al4CO4, Al2CO and Al4C3, respectively, thermodynamic analyses were used to study the chloride reaction production of them under the vacuum situation. The stable structures and electronic properties of AlCl, AlCl2 and AlCl3 adsorbed on Al4CO4, Al2CO and Al4C3 were calculated by first-principles calculations by the CASTEP module in the Materials Studio program. The results show that the AlCl3 and AlCl2 molecules have decomposed on the plane of Al4C3 (001), while there are no obvious decomposition of AlCl3 and AlCl2 on Al4CO4 (001) and Al2CO (001) planes. The adsorption of AlCl on the Al4CO4 (001) and Al2CO (001) planes is stronger than that on the Al4C3(001) plane. The interaction strength of AlCl3, as well as AlCl2, with Al4CO4, Al2CO and Al4C3 is in the sequence of Al4CO4Al2CO>Al4C3.

  10. Genetic diversity of Cuban pineapple germplasm assessed by AFLP Markers

    Directory of Open Access Journals (Sweden)

    Ermis Yanes Paz

    2012-01-01

    Full Text Available The Cuban pineapple germplasm collection represents the genetic diversity of pineapple cultivated in that country and includes other important genotypes obtained from the germplasm collections in Brazil and Martinique. The collection has previously been characterized with morphological descriptors but a molecular characterization has been lacking. With this aim, 56 six genotypes of A. comosus and one of Bromelia pinguin were analyzed with a total of 191 AFLP markers. A dendrogram that represents the genetic relationships between these samples based on the AFLP results showed a low level of diversity in the Cuban pineapple collection. All Ananas comosus accessions, being the majority obtained from farmers in different regions in Cuba, are grouped at distances lower than 0.20. Molecular characterization was in line with morphological characterization. These results are useful for breeding and conservation purposes.

  11. Bromelain enzyme from pineapple: in vitro activity study under different micropropagation conditions.

    Science.gov (United States)

    Vilanova Neta, Jaci Lima; da Silva Lédo, Ana; Lima, Aloisio André Bonfim; Santana, José Carlos Curvelo; Leite, Nadjma Souza; Ruzene, Denise Santos; Silva, Daniel Pereira; de Souza, Roberto Rodrigues

    2012-09-01

    The aim of this work was to evaluate the activity of bromelain in pineapple plants (Ananas comosus var. Comosus), Pérola cultivar, produced in vitro in different culture conditions. This enzyme, besides its pharmacological effects, is also employed in food industries, such as breweries and meat processing. In this work, the enzymatic activity was evaluated in the tissues of leaves and stems of plants grown in culture medium without plant growth regulator. The most significant levels of bromelain were observed in leaf tissue after 4 months of culture in vitro in medium with a filter paper bridge, followed by medium gelled by the agar. The results of this study, regarding the different structures of the pineapple (leaves and stems) in vitro showed that the activity of bromelain varied depending on the culture conditions, the time and structure of which was quantified, ensuring a viable strategy in the production of seedlings with high levels of bromelain in subsequent phases of micropropagation.

  12. De GPS al mapa

    Directory of Open Access Journals (Sweden)

    Esteban Dörries

    2016-03-01

    Full Text Available Las coordenadas Lambert obtenidas a partir de mediciones con equipos GPS de mano, llamados a veces navegadores, en ciertos casos confunden al usuario, por diferir claramente de su posición real al ser graficadas en un mapa del Instituto Geográfico Nacional (IGN: Esto puede resolverse con suficiente exactitud mediante una transformación de Molodensky, seguida de la correspondiente proyección cartográfica. Sin embargo, los tres parámetros necesarios para la transformación, supuestamente válidos para Costa Rica, se encuentran en muchas variantes y producen obviamente resultados diferentes. En este trabajo se analizan los fundamentos del problema y sus posibles soluciones, culminando con un estudio comparativo de ocho casos, que permite seleccionar los valores más adecuados para los parámetros.

  13. Derecho al desarrollo y globalidad

    OpenAIRE

    Álvarez Vita, Juan

    2014-01-01

    La presente disertación, que he intitulado Derecho al Desarrollo y Globalidad, consta de cuatropuntos principales:I. El derecho al desarrollo en el contexto de los derechos humanosII. La noción del derecho al desarrolloIII. El derecho al desarrollo en el marco de las llamadas generaciones de derechos humanosIV. El derecho al desarrollo y la aldea globalPalabras clave: derechos humanos, Derecho Natural, Derecho Positivo, sociedad global.

  14. Augmented Reality als Bildungsenhancement?

    OpenAIRE

    Damberger, Thomas

    2015-01-01

    Die Realität, die sich mithilfe von Datenbrillen und Smartphone Applikationen in die virtuelle Welt hinein ausdehnt, erfährt eine Form des Enhancements. Ein solches Enhancement kann unter bestimmten Voraussetzungen als Bildungsenhancement verstanden werden. Der Text befasst sich mit Formen der erweiterten Realität, ferner mit dem, um was es wesentlich bei der Bildung geht und zuletzt mit den Bedingungen, die erforderlich sind, um diesem Wesentlichen mit Hilfe von augmented reality besser zum ...

  15. Carta al editor

    Directory of Open Access Journals (Sweden)

    Harry Pachajoa

    2012-02-01

    Full Text Available Recently, Valencia et al., published a very interesting article called «Lipid profile in a group of patients with Turner’s syndrome at Clínica Universitaria Bolivariana in the city of Medellín between 2000 and 2009". An article which evaluated lipid levels in patients withTurner syndrome in a Colombian clinic, the study associated results of lipid profile with karyotype and other risk factors for coronary heart disease.

  16. Religion als soziale Deutungspraxis

    Directory of Open Access Journals (Sweden)

    Ulrike Lingen-Ali

    2016-12-01

    Full Text Available ENGLISH: During the last decades, the topos religion transformed into a powerful category of difference, which is also relevant for educationsystems and settings. Religion as a tool to differentiate between subjects is linked with power and dominance and, thus,with ideas about the constructed identities of “the other”. With this respects, religion functions as a code to define in- andexclusions and mark “the other”, e.g. “the Muslim” in predominantly Christian societies in Europe. Critical theories on racismenable to recognize, describe and define these practices and their effects. They can provide a useful tool to question and challengeintercultural and interreligious educational approaches which potentially reproduce and, thus, strengthen cultural/religiousdifferentiations. DEUTSCH: Die Differenz- und Heterogenitätskategorie Religion hat sich in den letzten Jahren im europäischen und auch deutschsprachigenDiskursraum zu einer medial, wissenschaftlich und politisch wirkmächtigen Kategorie entwickelt. Dabei zeigt sich Religion sowohlals soziale Unterscheidungspraxis (auch in pädagogischen Arrangements, als auch als Mittel zur Identifikation und Charakterisierungbestimmter Gruppen und Individuen als religiöse Subjekte. Religion stellt in diesem Zusammenhang einen Zugehörigkeitscodedar, mit dem Personen als natio-ethno-kulturell Andere markiert werden, wie die Markierung von MuslimInnen durchAngehörige der mehrheitlich christlichen Dominanzgesellschaft zeigt. Rassismuskritische Ansätze ermöglichen es zumeist eherimplizit an Rassekonstruktionen anschließende Unterscheidungen zu erkennen und zu beschreiben, den Bedingungen ihres Wirksamwerdensnachzugehen sowie ihre Konsequenzen zu bestimmen. Eine rassismuskritische und migrationspädagogisch informierteReligionspädagogik ermöglicht AkteurInnen, weniger auf gewaltvolle Unterscheidungspraxen angewiesen zu sein undalternative Veränderungs- und Handlungsmöglichkeiten zu

  17. Antioxidant effects of pineapple vinegar in reversing of paracetamol-induced liver damage in mice

    OpenAIRE

    Mohamad, Nurul Elyani; Yeap, Swee Keong; Lim, Kian Lam; Yusof, Hamidah Mohd; Beh, Boon Kee; Tan, Sheau Wei; Ho, Wan Yong; Sharifuddin, Shaiful Adzni; Jamaluddin, Anisah; Long, Kamariah; Nik Abd Rahman, Nik Mohd Afizan; Alitheen, Noorjahan Banu

    2015-01-01

    Background Pineapple (Ananas comosus) was demonstrated to be hepatoprotective. This study aims to investigate the reversing effects of pineapple vinegar on paracetamol-induced liver damage in murine model. Methods Pineapple juice was fermented via anaerobic and aerobic fermentation to produce pineapple vinegar. Male BALB/c mice (n = 70) were separated into 7 treatment groups (n = 10). Pineapple vinegar (0.08 and 2 mL/kg BW) and synthetic vinegar were used to treat paracetamol-induced liver da...

  18. Surface plasmon resonance optical sensor and antibacterial activities of biosynthesized silver nanoparticles

    Science.gov (United States)

    Bindhu, M. R.; Umadevi, M.

    2014-03-01

    Silver nanoparticles were prepared using aqueous fruit extract of Ananas comosus as reducing agent. These silver nanoparticles showed surface plasmon peak at 439 nm. They were monodispersed and spherical in shape with an average particle size of 10 nm. The crystallinity of these nanoparticles was evident from clear lattice fringes in the HRTEM images and bright circular spots in the SAED pattern. The antibacterial activities of prepared nanoparticles were found to be size-dependent, the smaller nanoparticles showing more bactericidal effect. Aqueous Zn2+ and Cu4+ selectivity and sensitivity study of this green synthesized nanoparticle was performed by optical sensor based surface plasmon resonance (SPR) at room temperature.

  19. ENZYMATIC KINETIC STUDY HYDROLASE FROM CITRUS

    Directory of Open Access Journals (Sweden)

    Israel Hernández

    2015-09-01

    Full Text Available In this paper the degrading activity of enzymes derived from orange peels (Citrus x sinensis, grapefruit (Citrus paradise and pineapple (Ananas comosus on the organic matter in wastewater is evaluated. This activity is measured indirectly by quantifying the biochemical oxygen demand (COD before and after degradation process based on a period of time using the HACH DR / 2010, and then the kinetic study was performed by the differential method and integral with the experimental data, obtaining a reaction order of 1 to pectinase (orange, and order 2 for bromelain (pineapple.

  20. [Occupational allergies to bromelain].

    Science.gov (United States)

    van Kampen, V; Merget, R; Brüning, T

    2007-03-01

    The protease bromelain originating from the pineapple fruit (Ananas comosus) finds frequent use in industry. Exposure to enzyme dusts has long been known to cause occupational allergies. The present paper reviews the results of the evaluation of literature data concerning occupational airway sensitization due to bromelain. Cases of specific airway sensitization caused by bromelain could be shown clearly by the presented studies. Since the symptoms, results of skin prick tests, detection of specific IgE antibodies and results of specific bronchoprovocation tests are consistent, an immunological mechanism can be assumed.

  1. Anatomical Structure of Stem and Adventitious Root Formation of Feijoa Cuttings%费约果茎解剖结构与插条不定根的形成

    Institute of Scientific and Technical Information of China (English)

    张猛; 王丹; 汤浩茹; 任少雄; 刘仁道

    2010-01-01

    @@ 费约果(Feijoa sellowiana)是桃金娘科多年生亚热带常绿灌木,原产于巴西东南部和乌拉圭,因其果实香味类似凤梨(Ananas comosus),又名凤梨番石榴(Thorp et al.,2002).费约果在新西兰、美国、法国、西班牙、俄罗斯、澳大利亚、日本等国有栽培,我国江苏、上海、四川、浙江等南方地区也有一定规模的种植.费约果作为一种集观赏、食用与药用为一体的新兴果树,在食品、药品、化妆品等领域具有广阔的应用前景(Nakashima,2001;Hardy et al.,1970;Kolesnik et al.,1991).在四川省外国专家局引智项目资助下,2004年,西南科技大学王丹教授从新西兰引进少量费约果苗木后,对其在四川地区的适应性以及综合性状进行了初步研究,认为费约果能够适应四川地区气候,并能保持其优良的食用与观赏特性,是一种极具发展潜力的食用兼观赏的树种(王丹等,2007).

  2. Teatro al aire libre

    Directory of Open Access Journals (Sweden)

    Philippe Canac

    2016-11-01

    Full Text Available Siendo cada vez más frecuentes y numerosas las peregrinaciones a la basílica de Notre Dame, de Laus (Altos Alpes, su capacidad resultaba hace tiempo insuficiente para acoger a sus peregrinos. Este fue el motivo de crear, en un terreno situado ante el atrio de la basílica, un conjunto, que permitiese celebrar misas al aire libre, ceremonias litúrgicas o representaciones de teatro religioso y que dispusiese de todas las posibilidades para conseguir la libertad del juego escénico.

  3. Rheocasting Al matrix composites

    Energy Technology Data Exchange (ETDEWEB)

    Girot, F.A.; Albingre, L.; Quenisset, J.M.; Naslain, R.

    1987-11-01

    A development status account is given for the rheocasting method of Al-alloy matrix/SiC-whisker composites, which involves the incorporation and homogeneous distribution of 8-15 vol pct of whiskers through the stirring of the semisolid matrix melt while retaining sufficient fluidity for casting. Both 1-, 3-, and 6-mm fibers of Nicalon SiC and and SiC whisker reinforcements have been experimentally investigated, with attention to the characterization of the resulting microstructures and the effects of fiber-matrix interactions. A thin silica layer is found at the whisker surface. 7 references.

  4. Frequently Asked Questions about ALS and the ALS Registry

    Science.gov (United States)

    ... Muscular Dystrophy Association (www.mda.org) The Les Turner ALS Foundation (www.lesturnerals.org) Back to Top ... als. Back to Top Why was incidence (new cases of ALS) not provided in the report? Date of diagnosis is needed to calculate incidence. ...

  5. Derivados vinculados al seguro.

    Directory of Open Access Journals (Sweden)

    De Paz Cobo, Sonia

    2003-01-01

    Full Text Available El presente trabajo tiene como objetivo la presentación de los derivados financieros como instrumentos de cobertura de los riesgos del seguro, así como de los principales proyectos que se desarrollan a través de los mismos, como por ejemplo los derivados sobre el clima. Hay que tener presente las pérdidas multimillonarias ocasionadas en casos como el de la banca Baring’s, o el de Orange County, por citar algunas, que si bien ciertos autores afirman que están en relación directa con el uso de estos productos, no podemos olvidar que los instrumentos derivados se desarrollaron con el objetivo principal de proteger al usuario de la posible volatilidad del activo subyacente. Ya en la industria aseguradora los derivados vinculados a tal actividad son instrumentos donde el pago se condiciona a la ocurrencia de su suceso de siniestro, o serie de sucesos múltiples que disparen las pérdidas. Tras el repaso efectuado y la descripción que este tipo de instrumentos puede tener en la gestión del riesgo por parte de la industria aseguradora, concluiremos con la exposición de las relaciones con el reaseguro tradicional así como una breve referencia al futuro de este tipo de instrumentos

  6. Gliricidia sepium al establecimiento

    Directory of Open Access Journals (Sweden)

    J. L. Valle

    2004-01-01

    Full Text Available Se asoció Cenchrus ciliaris (Cc y Gliricidia sepium (Gs para evaluar la producción de biomasa y la composición química de los forrajes en la fase de establecimiento, en el estado de Morelos, México, en condiciones de trópico seco. Se empleó un análisis de varianza con diseño en bloques al azar, en donde T1 fue pasto solo, T2 Cc más Gs con 5,000 plantas ha-1 y el T3 Cs más Gs con 14,285 plantas ha-1. Se midió la producción de forraje individual y asociado con materia seca (t MS/Ha, la altura (A en cm, la proteína cruda (PC%, las fracciones de fibra (FDN% y FDA% y la digestibilidad in vitro de la materia seca (DIVMS%. El T3 tuvo una mejor producción de biomasa (P0.05. La asociación de 14,285 plantas ha-1 de G. sepium con pasto Cenchrus ciliaris mejoró la producción de biomasa y la disponibilidad total de nutrientes por superficie cultivada al establecimiento

  7. AL-SIYAQ FI TARJAMAH KITAB AL-LUGHAH AL-'ARABIYAH ILA AL-LUGHAH AL-INDUNISIYAH

    Directory of Open Access Journals (Sweden)

    Devita Zuliati

    2016-06-01

    Full Text Available This study aimed at analyzing the translation of 53 poems in Tata Bahasa Arab book written by Chatibul Umam, et al. in view of the context theory. This study was based on a contextual translation theory that the understanding the right and proper context of Arabic texts translated into Indonesian plays an important role in determining the quality of a translation that is easily understood, contextual and functional. The qualitative data were read using the linguistic approach and critical translation. This study concluded that the translation work of Chatibul Umam, et al. tended to be literal not based on the complete understanding of the context, internal context (linguistic context and socio-cultural context of the texts. The role of the external context of the texts is less utilized in the translation process of poetry (nazham, so that the translation of the book is neither communicative nor functional.

  8. Modeling neuronal vulnerability in ALS.

    Science.gov (United States)

    Roselli, Francesco; Caroni, Pico

    2014-08-20

    Using computational models of motor neuron ion fluxes, firing properties, and energy requirements, Le Masson et al. (2014) reveal how local imbalances in energy homeostasis may self-amplify and contribute to neurodegeneration in ALS.

  9. AL-USRCRN Station Information

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Documentation of United States Alabama Regional Climate Reference Network (AL-USRCRN) installations in 2006. Installations documented are for AL-USRCRN pilot project...

  10. COMENTARIO AL CASO PINOCHET

    Directory of Open Access Journals (Sweden)

    Alberto Contreras Clunes

    2002-01-01

    Full Text Available El fallo en el caso Pinochet tiene distintas formas de ser analizado. De partida, desde un punto de vista político, también constitucional, del derecho penal. Sin embargo, nos abocaremos al estudio desde el punto de vista procesal, en especial a lo referido al Debido Proceso. Para ello se analizará los presupuestos, la solución dada por la Excma. Corte Suprema y las consecuencias del mismo. Además, se criticará la solución dada, desde la óptica de las garantías procesales del imputado, contenidas en el nuevo proceso penal y lo dispuesto en la Constitución y la Convención Americana de Derechos Humanos. Finalmente, se argumentará en favor de una interpretación finalista y armónica de la Constitución y los tratados internacionales de derechos humanos.The verdict of the Pinochet Case has different ways of being analyzed: from the political, the constitutional, and the penal law points of view. However, we will approach this study from the legal point of view, especially to that referred to as the Proper Process. To do this, the budgets, the solution given by the Most Excellent Supreme Court, and its consequences will be analyzed. Furthermore, the given solution will be criticized from the point of view of the legal guarantees of the accused contained in the new penal process and what is contained in the Constitution and in the American Convention of Human Rights. In conclusion, we will argue in favor of a final and harmonious interpretation of the Constitution and the international agreement of human rights.

  11. Carta al Editor

    Directory of Open Access Journals (Sweden)

    Darío Maldonado Romero

    1991-03-01

    Full Text Available

    Bogotá, 01 de Agosto de 1990
    Señores
    Miembros del Consejo Editorial
    Academia Nacional de Medicina
    E.S.D.

    Señores doctores:

    Con toda consideración y respeto me permito agregar algunas anotaciones al escrito del Académico Alberto Cárdenas Escobar, aparecido en el No. 22 de Medicina, para destacar algunos aspectos de la personalidad del doctor Alberto Schweitzer galardonado con el Premio Nobel de la Paz.

    A los 30 años de edad tomó una decisión dramática: Estudiar Medicina para trabajaren el África Ecuatorial. Cuando terminó sus investigaciones sobre enfermedades tropicales y sus prácticas hospitaLarias, partió para el Gabon al pequeño pueblo de Lambarené. La tarea era desconsoladora; La atención médica había que prestarla al aire libre; iba con su esposa, como enfermera y amanuense, y no se encontraba ni un intérprete, ni un ayudante. Cuando le llegaron la medicina y el equipaje hubo que acomodarlos en un viejo gallinero abandonado; el calor era insoportable y le venían enfermos de varias millas a la redonda, a pie o en Canoas.

    Las enfermedades más difundidas eran Malaria, Disentería, estreptococias y la más mortífera, la Tripanosomiasis Gambiense, para la que empezaban a llegar medicinas nuevas eficaces y las medidas preventivas como el suministro de agua potable y enmallado de ventanas impedían muchas contaminaciones.

    Pero la que causaba mayores dificultades y trabajo era la Mycobacteriasis Anestesiante, incurable, de difícil diagnóstico, afectando a varios miembros de la misma familia, de aspecto horripilante, con complicaciones neuríticas muy dolorosas, frecuente ceguera, e invalideces y procesos erisepelatososacrotéricos, que exigían acompañante para el cambio y lavado de vendas y suministro de alimentos.

    A menos de un año de

  12. Konsep Hati Menurut al-Hakim al-Tirmidzi

    Directory of Open Access Journals (Sweden)

    Ryandi Ryandi

    2014-03-01

    Full Text Available Dalam khazanah sufi, hati atau qalb adalah salah satu term sentral yang dibahas secara mendalam. Karena bagi sufi, hati adalah entitas metafisik (latifah rabbaniyyah ruhaniyyah yang dengannya manusia dapat mencapai ma’arifatullah dan mengetahui rahasia-rahasia-Nya. Salah satu sufi klasik yang mengkaji hati secara mendalam adalah Abu ‘Abdullah ibn ‘Ali ibn al-Hasan ibn Basyar al-Hakim al-Tirmidzi (w 320 H. Ia mengkonsepsikan hati sebagai entitas metafisik universal yang terkandung di dalamnya tingkatan-tingkatan batin (maqamat al-qalb, yaitu: sadr, qalb, fu’ad, dan lubb. Pemetaan ini dimunculkan sesuai dengan fungsi linguistiknya dan penggunaannya dalam al-Qur’an dan Hadis Nabi SAW. Tiap-tiap tingkatan batin tersebut berkaitan dengan tingkatan pengetahuan, keilmuan, spiritual, dan jiwa manusia. Kaitan tersebut menunjukan bahwa qalb merupakan eksistensi ruhani manusia (al-kainunah al-ruhiyyah, yang berfungsi sebagai instrumen penyempurna bagi manusia (al-jihaz al-mutakamil li al-insan yang meliputi seluruh kekuatan dan potensi manusia: ruhani, ‘aqliyah, dan kehendak, di mana manusia dengannya dapat merasa, berpikir, mengetahui, bahkan dapat mencapai ma’rifatullah dan dekat dengan-Nya.

  13. Editorial: Cartas al Editor

    Directory of Open Access Journals (Sweden)

    Guillermo Llanos

    2006-06-01

    Full Text Available En todas las revistas biomédicas del mundo existe una sección habitual denominada como el título de este editorial y que en Colombia Médica la llamamos correspondencia. Este espacio tiene como objetivo fundamental que los lectores escriban a los autores (investigadores comentando los artículos que se han publicado en números anteriores de la revista. Las cartas son, entonces, breves comentarios de desacuerdo o acuerdo (conceptual, metodológico, interpretativo sobre falencias ubicadas por un lector alerta al tema debatido en cualquiera de las secciones como en los artículos originales, editoriales, revisiones, presentación de casos, etc. publicados previamente en la revista. Las cartas deben permitir el intercambio de opiniones y formas de pensar y analizar los problemas planteados entre los autores y los lectores. En ocasiones una serie de cartas motivadas por un artículo original puede ser tan o más interesante como el artículo que generó la correspondencia. Las cartas son también un formato adecuado para comunicar un hecho novedoso de una observación clínica juiciosa no descrita antes. Un ejemplo de ello es la carta de un gineco-obstetra australiano cuando informó a Lancet los primeros efectos adversos informados de la talidomida. Colombia Médica, a pesar de mantener vigente esta sección, no ha sido muy afortunada en establecer una buena cultura del envío de cartas al editor. Pero últimamente han llegado juiciosas observaciones a las cuales se les ha dado prioridad en su publicación. Toda carta que comenta los hallazgos de un artículo es enviada a los autores para su respuesta, porque ello genera el debate académico buscado, y luego se publican, tanto la carta como su respuesta. El Estilo Vancouver recomienda que la carta y su respuesta deban ser breve, no pasar de dos o tres párrafos y contener sólo cuatro o cinco referencias. En lo posible (a menos que sea indispensable no presentar ni cuadros ni gráficos. El t

  14. Malamih al-Ashwat al-'Arabiyyah wa Makharijaha

    Directory of Open Access Journals (Sweden)

    Amrah Muhammad Kasim

    2015-12-01

    Full Text Available This writing discusses on al-ashwat al-‘arabiyyah that is known with “phonetic” term. It talks and interprets makharij al-huruf or place for producing sounds and also processing production of sounds by parts of mouth in Arabic language. The problems of sounds and its systems in Arabic language have been conducted by the scholars of al-ashwat al-‘arabiyyah attractively, neatly, and detail and gotten ahead the phonetic western scholars. Part of their analysis results formulate the study about al-ashwat al-sakinah (al-Shamit is known with vocal and consonant sounds in research questions shawait – shawamit. Ibnu Jinni has formulated “harakat”(wowel in Arabic language and dimension of letter  mad and liyn on alif  letter - waw - ya’ (ا و ي, from these letters create  harakat fathah, dammah, and kasrah. Moreover, in this writing also exist the description of hija’i letters and its makhraj that are correct from parts of mouth and the unity of coordinative sounds that produce sounds in morphemes arrangement, in one word that produces meaning. The result of phonological study has divided al-ashwat to be kinds of sound like the pronounced sounds, produced sounds from mouth, and heard sound.

  15. Porosity of porous Al alloys

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    Two porosity models of porous Al alloys with different pore types (ball and polygon shape) were established. The experimental results coincide well with theoretical computations. The porosity of Al alloys (Prc) consists of three parts, porosity caused by preform particles (Prp), additional porosity (Pra), and porosity caused by solidification shrinkage (Prs). Prp is the main part of Prc while Pra is the key for fabricating porous Al alloys successfully in spite of its little contribution to Prc.

  16. Al versus Si competition in FeSiAl alloys

    Energy Technology Data Exchange (ETDEWEB)

    Legarra, E. [Dpto. Electricidad y Electronica, Facultad de Ciencia y Tecnologia, Universidad del Pais Vasco (UPV/EHU), CP 644, 48080 Bilbao (Spain)], E-mail: estibaliz.legarra@ehu.es; Apinaniz, E. [Dpto. Fisica Aplicada I, Escuela de Ingenieria Tecnica Superior, Universidad del Pais Vasco, Alameda de Urquijo s/n 48013 Bilbao (Spain); Plazaola, F. [Dpto. Electricidad y Electronica, Facultad de Ciencia y Tecnologia, Universidad del Pais Vasco (UPV/EHU), CP 644, 48080 Bilbao (Spain); Jimenez, J.A. [Centro Nacional de Investigaciones Metalurgicas (CENIM), Avda. Gregorio del amo 8, 28040 Madrid (Spain); Pierna, A.R. [Chemical Engineering and Environmental Department, UPV/EHU, Box 1379, 20008 San Sebastian (Spain)

    2008-10-15

    In FeSiAl alloys, when Si substitutes for Al, important changes take place in the magnetism as well as in the structural properties. Alloys in the two composition series Fe{sub 75}Al{sub 25-x}Si{sub x} (x=0, 7.5, 12.5, 17.5, 25) and Fe{sub 70}Al{sub 30-x}Si{sub x} (x=0, 9, 15, 21, 30) were prepared by induction melting; afterwards they were crushed and then annealed in order to recover the DO{sub 3} stable phase. The deformed FeAl samples show larger lattice parameters than the ordered ones; however, this difference ({delta}a) decreases when Si substitutes for Al until it becomes zero (i.e. until the ordered samples and the deformed ones have the same lattice parameters). This trend is the same for both sample series and does not depend on the Fe content of the alloy. However, the magnetization has a different behaviour depending on the Fe content. For deformed Fe{sub 75}Al{sub 25-x}Si{sub x} alloys the saturation magnetization decreases with increasing Si content while for Fe{sub 70}Al{sub 30-x}Si{sub x} deformed alloys the saturation magnetization has a plateau in which the saturation magnetization values do not vary.

  17. Thermal Modeling of Al-Al and Al-Steel Friction Stir Spot Welding

    Science.gov (United States)

    Jedrasiak, P.; Shercliff, H. R.; Reilly, A.; McShane, G. J.; Chen, Y. C.; Wang, L.; Robson, J.; Prangnell, P.

    2016-09-01

    This paper presents a finite element thermal model for similar and dissimilar alloy friction stir spot welding (FSSW). The model is calibrated and validated using instrumented lap joints in Al-Al and Al-Fe automotive sheet alloys. The model successfully predicts the thermal histories for a range of process conditions. The resulting temperature histories are used to predict the growth of intermetallic phases at the interface in Al-Fe welds. Temperature predictions were used to study the evolution of hardness of a precipitation-hardened aluminum alloy during post-weld aging after FSSW.

  18. Philosophieren als Unterrichtsprinzip im Mathematikunterricht

    Science.gov (United States)

    Meerwaldt, Diana

    Philosophieren und Mathematik scheinen zunächst gegensätzliche Bereiche zu sein, die sich kaum vereinbaren lassen. Dies trifft für eine Auffassung zu, die Philosophieren als "Gerede" disqualifiziert und Mathematik als eine reine "Formelwissenschaft" begreift. Beide Auffassungen werden den Gegenständen nicht gerecht.

  19. El miedo al rey

    Directory of Open Access Journals (Sweden)

    François FORONDA

    2007-01-01

    Full Text Available La peur du roi. Sources et premières réflexions à propos d’une émotion aristocratique dans la Castille du XIVe siècleFace à un roi qui à présent pousse les effets de son ire jusqu’au meurtre pour faire le vide autour de lui et imposer sa différence souveraine, ses parents et les riches hommes, soit les principaux visés par cette politique de l’arbitraire qui enfreint les règles du jeu autant qu’elle contribue à les redéfinir, disent la peur (metus qu’ils ressentent, qu’ils utilisent aussi afin de donner à leur stratégie de pouvoir un fondement légitime. Et l’expression de cette émotion politique, qui tend à se muer en un stéréotype d’un discours aristocratique d’opposition, surtout présente dans les chroniques, mises ici en relation ici avec d’autres sources, notamment des correspondances, marque profondément l’histoire des relations souvent conflictuelles au sein de cette compagnie en définitive très étroite pendant un siècle, de la fin du XIIIe siècle à la fin du XIVe siècle. Cet article se propose d’ébaucher une première réflexion sur le sujet et d’en présenter les principales sources.El miedo al rey. Fuentes y primeras reflexiones acerca de una emoción aristocrática en la Castilla del siglo XIVFrente a un rey que ahora lleva hasta la muerte los efectos de su ira para hacer el vacío entorno a su persona e imponer su soberana, sus parientes y ricos hombres, o sea los principales afectados por esta política de la arbitrariedad que rompe las reglas del juego a la vez que contribuye a redefinirlas, dicen el miedo (metus que sienten, que utilizan también para darle a su estrategia de poder un fundamento legítimo. Y la expresión de esta emoción política, que tiende a convertirse en un estereotipo de un discurso aristocrático de oposición, sobre todo presente en la crónicas, aquí puestas en relación con otras fuentes, en particular cartas, marca profundamente la historia de

  20. Cell wall alterations in the leaves of fusariosis-resistant and susceptible pineapple cultivars.

    Science.gov (United States)

    de Farias Viégas Aquije, Glória Maria; Zorzal, Poliana Belisário; Buss, David Shaun; Ventura, José Aires; Fernandes, Patricia Machado Bueno; Fernandes, Antonio Alberto Ribeiro

    2010-10-01

    Fusariosis, caused by the fungus Fusarium subglutinans f. sp. ananas (Syn. F. guttiforme), is one of the main phytosanitary threats to pineapple (Ananas comosus var. comosus). Identification of plant cell responses to pathogens is important in understanding the plant-pathogen relationship and establishing strategies to improve and select resistant cultivars. Studies of the structural properties and phenolic content of cell walls in resistant (Vitoria) and susceptible (Perola) pineapple cultivars, related to resistance to the fungus, were performed. The non-chlorophyll base of physiologically mature leaves was inoculated with a conidia suspension. Analyses were performed post-inoculation by light, atomic force, scanning and transmission electron microscopy, and measurement of cell wall-bound phenolic compounds. Non-inoculated leaves were used as controls to define the constitutive tissue characteristics. Analyses indicated that morphological differences, such as cell wall thickness, cicatrization process and lignification, were related to resistance to the pathogen. Atomic force microscopy indicated a considerable difference in the mechanical properties of the resistant and susceptible cultivars, with more structural integrity, associated with higher levels of cell wall-bound phenolics, found in the resistant cultivar. p-Coumaric and ferulic acids were shown to be the major phenolics bound to the cell walls and were found in higher amounts in the resistant cultivar. Leaves of the resistant cultivar had reduced fungal penetration and a faster and more effective cicatrization response compared to the susceptible cultivar.

  1. Quantum dots formed in InSb/AlAs and AlSb/AlAs heterostructures

    Science.gov (United States)

    Abramkin, D. S.; Rumynin, K. M.; Bakarov, A. K.; Kolotovkina, D. A.; Gutakovskii, A. K.; Shamirzaev, T. S.

    2016-06-01

    The crystal structure of new self-assembled InSb/AlAs and AlSb/AlAs quantum dots grown by molecularbeam epitaxy has been investigated by transmission electron microscopy. The theoretical calculations of the energy spectrum of the quantum dots have been supplemented by the experimental data on the steady-state and time-resolved photoluminescence spectroscopy. Deposition of 1.5 ML of InSb or AlSb on the AlAs surface carried out in the regime of atomic-layer epitaxy leads to the formation of pseudomorphically strained quantum dots composed of InAlSbAs and AlSbAs alloys, respectively. The quantum dots can have the type-I and type-II energy spectra depending on the composition of the alloy. The ground hole state in the quantum dot belongs to the heavy-hole band and the localization energy of holes is much higher than that of electrons. The ground electron state in the type-I quantum dots belongs to the indirect X XY valley of the conduction band of the alloy. The ground electron state in the type-II quantum dots belongs to the indirect X valley of the conduction band of the AlAs matrix.

  2. Concentration by membrane separation processes of a medicinal product obtained from pineapple pulp

    Directory of Open Access Journals (Sweden)

    Francisco Luiz Gumes Lopes

    2009-04-01

    Full Text Available The concentration of pineapple juice is needed to retain the bromelain activity and to standardize the composition and proteolytic activity. Thus, this work aimed to obtain a pure bromelain extract from the Ananas comosus L. Merril juice by membrane separation process. A 2² experimental planning was used to study the influence of pH and transmembrane pressure on the activity recovery by micro-filtration using a plain membrane. In second step, this enzyme was purified by the ultra-filtration using a 10 kDa millipore kit. The best operation condition to bromelain concentration using the plain membrane was at pH 7.5 and transmembrane pressure of 0.05 bar, while 85% of bromelain activity was recovered. Ultra-filtration retained 100% of proteolytic activity and concentrated in 10 fold the bromelain extract. SDS-PAGE electrophoresis showed that the ultra-filtrated had high purity and the bromelain from A. comosus pulp had a molecular weight of 24.5 kDa.A concentração do suco de abacaxi é necessária para manter a atividade da bromelina e padronizar a composição e atividade proteolítica. Assim, este trabalho objetivou a obter um extrato de bromelina pura do suco do Ananas comosus L. Merril por processos de separação por membranas. Um planejamento experimental do tipo 2² foi feito para estudar a influência do pH a da pressão transmembranar sobre a recuperação da atividade por micro-filtração usando uma membrana plana. Em uma segunda etapa, purificou-se a enzima alvo por ultra-filtração usando um "kit millipore" de 10 kDa. A melhor condição para a concentração da bromelina foi a pH 7,5 e pressão transmembranar de 0,05 bar, onde 85% da atividade da bromelina foi recuperado. A ultra-filtração manteve 100% da atividade proteolítica e concentrou em 10 vezes o extrato de bromelina. A eletroforese via SDS-PAGE mostrou que o ultra-filtrado teve alta pureza e a bromelina da polpa do Ananas comosus tem um peso molecular de 24.5 kDa.

  3. EKSKURSO AL CANGYAN-MONTO

    Institute of Scientific and Technical Information of China (English)

    Verkita; de; Li; Shijun

    1999-01-01

    La Pekina Esperanto-Asocio preskaǚ sta-rigis tradicion organizi ekskurson printempe kajaǚtune. En la pasinta jaro, ni ekskursis al In-terna Mongolio kaj habis agrablajn renkontiojnkun geesperantistoj en Huhehot kaj Baotou. i-

  4. Al clamor de les vuvuzeles

    Directory of Open Access Journals (Sweden)

    Javier Olivera Betrán

    2010-09-01

    Full Text Available El Mundial de Futbol de Sud-àfrica ha situat l’Àfrica al mapa mundial i ha mostrat al món el triomf de l’estil de futbol d’Espanya basat en la col·lectivitat, l’esperit d’equip, la humilitat, la cohesió interna i el joc bonic. L’organització africana, seriosa i eficaç, ha sabut mobilitzar i il·lusionar la població del país que ha participat en el mundial des de l’orgull de ser africà, amb l’esperit alegre, festiu i també reivindicatiu de la gent de l’Àfrica. Aquest esdeveniment suposa el llançament de la marca “Sud-àfrica” al món amb un avís clar d’optar a l’organització d’uns primers Jocs Olímpics a Àfrica (potser els de 2020. S’obre una finestra d’esperança al segle xxi per al continent perdut de la mà de l’esport espectacle tan universal, massiu, emocional i mercantil que també es juga a la canxa de la política i de l’economia.

  5. CARACTERIZAÇÃO AGRONÔMICA E DIVERGÊNCIA GENÉTICA ENTRE ACESSOS DE ABACAXIZEIRO NAS CONDIÇÕES DO ESTADO DE MATO GROSSO

    Directory of Open Access Journals (Sweden)

    ALINE VIDOR MELÃO

    2015-12-01

    Full Text Available RESUMO Para iniciar um programa de melhoramento genético, a existência da variabilidade genética é essencial. Assim, a caracterização dos genótipos é o primeiro passo para adefinição da estratégia de melhoramento a ser adotada. Neste sentido, este trabalho teve como objetivo estudar a divergência genética, com base em caracteres morfoagronômicos, entre 19 acessos de abacaxizeiro (vAnanas comosusar. comosus pertencentes à coleção ativa de trabalho da UNEMAT de Tangará da Serra (MT. Foram utilizados 52 descritores, sendo 31 descritores qualitativos e 21 quantitativos. Para aanálise dos dados, utilizou-se do coeficiente de coincidência simples, da distância euclidiana média padronizada e da análise conjunta por meio da distância de Gower. Pelos descritores avaliados, verificou-se amplavariabilidade entre os acessos de A. comosus var. comosus. A análise conjunta foi mais eficiente na representação da variabilidade genética entre os acessos avaliados. Sugerem-se os cruzamentos dos acessos 4; 5; 6; 7; 8; 13 e 19 com os acessos 3; 9; 10 e 11.

  6. Al Zubarah - eine vergessene Legende

    DEFF Research Database (Denmark)

    Kinzel, Moritz

    2014-01-01

    Die im 18. Jahnhundert an der Nordwestküste Katars entstandene Handelsstadt Al Zubarah bildet den Forschungsschwerpunkt des Qatar Islamic Archaeology and Heritage Projects (QIAH), das die Universität Kopenhagen in Zusammenarbeit mit der Katarischen Museumsbehörde (QMA) seit 2009 durchführt. Neben...... der archäologischen Erforschung der Stadtanlage und ihrer Elemente, stehen die Erhaltung und Präsentation des Fundortes und seiner Bauten von Anfang an ebenfalls im Fokus der Arbeiten. Auf Grundlage der Projektarbeit wurde 2011 der Antrag auf Aufnahme von Al Zubarah in die Weltkulturerbe eingereicht....... Die Stadtanlage ist ein einzigartiges Beispiel für eine planmäßig angelegte Stadt mit allen typischen Elementen einer orientalischen Stadt: Souq, Moschee, Hofhausquartiere und turmbewehrter Stadtmauer. Die Blütezeit von Al Zubarah währte nur gut 50 Jahre. 1811 wurde sie bereits wieder zerstört. Einige...

  7. Conjuntivitis alérgica

    Directory of Open Access Journals (Sweden)

    Alejandro Tello Hernández

    2008-07-01

    Full Text Available La conjuntivitis alérgica es una de las causas más comunesde consulta externa en oftalmología y tiene una muy altarelación con las reacciones alérgicas sistémicas. Lafisiopatología de las reacciones de hipersensibilidad noshace entender los cuadros clínicos característicos de estapatología y sus diferentes grados de severidad. Eldiagnostico diferencial es amplio, sin embargo hay clavesdiagnosticas que nos orientan rápidamente al diagnosticocorrecto. El tratamiento se enfoca en las medidasfarmacológicas y no farmacológicas, utilizándolas enconjunto para disminuir la recurrencia del cuadro y prevenirsecuelas visuales.

  8. Superconductivity in Al/Al2O3 interface

    Science.gov (United States)

    Palnichenko, A. V.; Vyaselev, O. M.; Mazilkin, A. A.; Khasanov, S. S.

    2016-06-01

    Metastable superconductivity at Tc ≈ 65 K has been observed in Al foil subjected to special oxidation process, according to the ac magnetic susceptibility and electrical resistance measurements. Comparison of the ac susceptibility and the dc magnetization measurements infers that the superconductivity arises within the interfacial granular layer formed during the oxidation process between metallic aluminum and its oxide.

  9. FTD and ALS: genetic ties that bind.

    Science.gov (United States)

    Orr, Harry T

    2011-10-20

    Curiously, amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD), seemingly disparate neurodegenerative disorders, can be inherited together. Two groups (DeJesus-Hernandez et al. and Renton et al.) show that the long sought after ALS/FTD mutation on chromosomal region 9p is a hexanucleotide expansion in C90RF72. These studies, plus a study on X-linked ALS/FTD, provide molecular starting points for identifying pathways that link ALS and FTD pathogenesis.

  10. Drogodependencia y derecho al honor

    OpenAIRE

    Moliner Navarro, Rosa María

    2014-01-01

    Resulta frecuente hoy la divulgación de la drogodependencia de algunos personajes públicos en los medios que se dedican a la información social. Acudiendo a la jurisprudencia más reciente en la materia, el artículo analiza el habitual conflicto entre el derecho al honor y el derecho a la libertad de información; identifica los criterios actualmente vigentes en la protección del derecho al honor y señala el límite que la libertad de información nunca puede traspasar en la revela...

  11. Al-Qaeda Since 911

    OpenAIRE

    Brahimi, Alia

    2012-01-01

    Seconds after smiling at her local MP and shaking his hand, Roshonara Choudhry plunged a knife into his stomach. The attack on Stephen Timms in May 2010 was not the most high profile assault inspired by al- Qaeda, nor was it fatal. It did, however, offer a commentary on the changing nature of the terrorist threat since 9/11, al-Qaeda’s major impact on international relations as well as its major failure, and the intimate, perhaps inevitable, connection between the two.

  12. La neuroplasticidad aplicada al deporte

    OpenAIRE

    Fernández, Hernán

    2015-01-01

    Me decidí a realizar la siguiente ponencia, para analizar y reconocer la importancia que implica el trabajo cognitivo en el entrenamiento deportivo. El abordaje que efectuaré, está enfocado en el desarrollo del entrenamiento cognitivo, su fundamento y el desarrollo de la neuroplasticidad. Analizaré el concepto de neuroplasticidad y neurogénesis como base al sustento teórico de la neurociencia aplicada al deporte. También remarcaré diversas metodologías prácticas, con su fundamento teóri...

  13. ¿COLOMBIA DEBE APOSTARLE AL SOFTWARE LIBRE, AL DE FUENTE ABIERTA O AL PROPIETARIO?

    Directory of Open Access Journals (Sweden)

    Luis Alberto Girón Murillo

    2014-06-01

    Full Text Available Es complejo para gestores y administradores conocer las implicaciones que la adopción de modelos de desarrollo de software tendría para una región. Este artículo ofrece una evaluación de las implicaciones dinámicas que para el desarrollo de software de una región tendría el apostar de forma excluyente al software libre, al software de fuente abierta y al software propietario. Se desarrolló un modelo de simulación en Vensim de la dinámica de sistemas en donde se evaluaron 3 escenarios: uno en donde se adopta de forma excluyente el software libre, uno en donde se adopta el software propietario y uno en donde se adopta el software de fuente abierta. Se ofrecen descripciones y explicaciones de los resultados que sugieren las potencialidades de cada uno de los modelos de desarrollo evaluados.

  14. Un controesempio al Modus Ponens

    Directory of Open Access Journals (Sweden)

    Vann McGee

    2011-11-01

    Full Text Available La traduzione dell’articolo è stata resa possibile grazie al consenso dell’autore e del Journal of Philosophy. Riferimenti originali: Vann McGee (1985: “A Counterexample to Modus Ponens”, Journal of Philosophy, Vol. 82, No. 9 (Sep. 1985, pp. 462-47. [N.d.T.

  15. Der Nutzer als besserer Fachreferent?

    Directory of Open Access Journals (Sweden)

    Robert Scheuble

    2014-12-01

    Full Text Available Am 9. Juli 2014 veranstaltete der VDB-Regionalverband Südwest in Kooperation mit der Deutschen Nationalbibliothek in Frankfurt am Main eine Fortbildungsveranstaltung zum Thema „Der Nutzer als besserer Fachreferent?”, die sich mit Formen von und Erfahrungen mit nutzergesteuerter Erwerbung (PDA in wissenschaftlichen Bibliotheken beschäftigte.

  16. Kringloop als basis van bedrijfsvoering

    NARCIS (Netherlands)

    Linde, van der A.

    2007-01-01

    Gerard Keurentjes bekijkt zijn bedrijf als kringloop waarin grond en koeien de spil zijn. Het gebruik van microbiotica in plaats van antibiotica past daarin perfect. Een microbioticum bestaat uit een mengsel van acht kruiden opgelost in alcohol. Onderzoek toont aan dat het de productie van witte blo

  17. Al Zubarah - eine vergessene Legende

    DEFF Research Database (Denmark)

    Kinzel, Moritz

    2014-01-01

    Jahre später auf einem Sechstel der ursprünglichen Siedlungsfläche wiedererrichtet und dann im ausgehenden 19. Jahrhundert / beginnenden 20. Jahrhundert endgültig aufgeben. Die Stadt lebt als Legende im Bewusstsein der katarischen Bevölkerung, doch der Name wird heute nicht mit der Stadtanlage sondern...

  18. MOOCs; vanwaar al die ophef?

    NARCIS (Netherlands)

    Henderikx, Maartje; Kreijns, Karel

    2016-01-01

    MOOCs; vanwaar die ophef? Het nog maar zeer recente fenomeen MOOCs (massive open online courses) heeft zich in korte tijd al een onwrikbare positie weten in te nemen in het open online onderwijs. De hype rondom deze nieuwe manier van onderwijs geven bereikte zijn hoogtepunt in 2012, dat prompt werd

  19. First principles calculation of ac conductance for Al-BDT-Al and Al-Cn-Al systems

    Directory of Open Access Journals (Sweden)

    Jia-Ning Zhuang

    2011-12-01

    Full Text Available We perform first-principles calculation to investigate the dynamic conductance of atomic wires of the benzenedithiol (BDT as well as carbon chains with different length in contact with two Al(100 electrodes (Al-Cn-Al. Our calculation is based on the combination of the non-equilibrium Green's function and the density functional theory. For ac conductance, there are two theories that ensures the current conservation: (1. the global formula which is a phenomenological theory that partitions the total displacement current into each leads so that the current is conserved.(2. the local formula which is a microscopic theory that includes Coulomb interaction explicitly so that the current is conserved automatically. In this work, we use the local formula to calculate the dynamic conductance, especially the emittance. We give a detailed comparison and analysis for the results obtained from two theories. Our numerical results show that the global formula overestimates the emittance by two orders of magnitude. We also obtain an inequality showing that the emittance from global formula is greater than that from local formula for real atomic structures. For Al-Cn-Al structures, the oscillatory behavior as the number of carbon chain N varies from even to odd remains unchanged when local formula is used. However, the prediction of local formula gives rise to opposite response when N is odd (inductive-like as compared with that of global formula. Therefore, one should use the local formula for an accurate description of ac transport in nanoscale structures. In addition, the ‘size effect’ of the ac emittance is analyzed and can be understood by the kinetic inductance. Since numerical calculation using the global formula can be performed in orbital space while the local formula can only be used in real space, our numerical results indicate that the calculation using the local formula is extremely computational demanding.

  20. Personalidad y tolerancia al dolor

    Directory of Open Access Journals (Sweden)

    JESÚS M.ª CARRILLO

    2005-01-01

    Full Text Available Se examina la relación de algunas variables de personalidad -EPQ-A (Eysenck y Eysenck, 1975, NEO-PI (McCrae y Costa, 1988 y STA -personalidad esquizotípica- y STB -personalidad límite-, de Claridge y Broks (1984, con la tolerancia al dolor experimental inducido mediante agua fría (cold pressor test, según el procedimiento de Staats, Heckmat y Staats (1998 en una muestra filtrada mediante un cuestionario sobre dolor crónico, dolores de cabeza, de espalda, artritis, síndrome de Raynaud, y personas bajo medicación. La tolerancia al dolor experimental se evaluó mediante el Umbral del dolor (tiempo en segundos desde la introducción de la mano en el agua hasta el primer informe de dolor, Mantenimiento del dolor (duración en segundos de la inmersión de la mano desde la aparición del umbral hasta su retirada y Tolerancia al dolor (duración total en segundos de la inmersión de la mano desde su introducción hasta su retirada. Un análisis correlacional, factorial y de regresión por pasos mostraron un efecto cruzado entre medidas de ansiedad (tolerancia negativa al dolor y psicoticismo, siendo psicoticismo junto con control emocional predictores de tolerancia positiva al dolor. En cuanto a psicoticismo, parece que son sus elementos de naturaleza paranoide y de "dureza" los que afectan a una percepción atenuada del dolor.

  1. Large rectification magnetoresistance in nonmagnetic Al/Ge/Al heterojunctions.

    Science.gov (United States)

    Zhang, Kun; Li, Huan-Huan; Grünberg, Peter; Li, Qiang; Ye, Sheng-Tao; Tian, Yu-Feng; Yan, Shi-Shen; Lin, Zhao-Jun; Kang, Shi-Shou; Chen, Yan-Xue; Liu, Guo-Lei; Mei, Liang-Mo

    2015-09-21

    Magnetoresistance and rectification are two fundamental physical properties of heterojunctions and respectively have wide applications in spintronics devices. Being different from the well known various magnetoresistance effects, here we report a brand new large magnetoresistance that can be regarded as rectification magnetoresistance: the application of a pure small sinusoidal alternating-current to the nonmagnetic Al/Ge Schottky heterojunctions can generate a significant direct-current voltage, and this rectification voltage strongly varies with the external magnetic field. We find that the rectification magnetoresistance in Al/Ge Schottky heterojunctions is as large as 250% at room temperature, which is greatly enhanced as compared with the conventional magnetoresistance of 70%. The findings of rectification magnetoresistance open the way to the new nonmagnetic Ge-based spintronics devices of large rectification magnetoresistance at ambient temperature under the alternating-current due to the simultaneous implementation of the rectification and magnetoresistance in the same devices.

  2. Effect of interface geometry on electron tunnelling in Al/Al2O3/Al junctions

    Science.gov (United States)

    Koberidze, M.; Feshchenko, A. V.; Puska, M. J.; Nieminen, R. M.; Pekola, J. P.

    2016-04-01

    We investigate how different interface geometries of an Al/Al2O3 junction, a common component of modern tunnel devices, affect electron transport through the tunnel barrier. We study six distinct Al/Al2O3 interfaces which differ in stacking sequences of the metal and the oxide surface atoms and the oxide termination. To construct model potential barrier profiles for each examined geometry, we rely on first-principles density-functional theory (DFT) calculations for the barrier heights and the shapes of the interface regions as well as on experimental data for the barrier widths. We show that even tiny variations in the atomic arrangement at the interface cause significant changes in the tunnel barrier parameters and, consequently, in electron transport properties. Especially, we find that variations in the crucial barrier heights and widths can be as large as 2 eV and 5 Å, respectively. Finally, to gain information about the average properties of the measured junction, we fit the conductance calculated within the Wentzel-Kramers-Brillouin approximation to the experimental data and interpret the fit parameters with the help of the DFT results.

  3. ATSAR THARIQAH AL-QIRA'AH AL-MUTAKARRIRAH FI MAHARAH AL-KITABAH

    Directory of Open Access Journals (Sweden)

    Rihlah

    2016-06-01

    Full Text Available This study aimed to reveal the students' writing skill, namely dictation (Imlâ’ and to analyze the use of the repeated reading technique in learning Qirâ'ah Jahriyyah, as well as to determine the effect of the use of al-Qirâ'ah al-Mutakarrirah technique on the students' skills in writing dictation. This method was used to improve students’ reading fluency at the beginners’ level, which was popularized by Samuel in 1979. This research used the experimental method through pretest and posttest control group design. The samples of the research were 44 students consisted of 22 students the experimental class and 22 students of the control class. The analysis of the data applied t-test. The results showed that the use of was significant on the students' writing skill in dictation. It was based on the tcount, 2.64, and ttable, 2.02, at the significance level of 5%. The al-Qirâ'ah al-Mutakarrirah technique was thus effectively used to develop the beginner-leveled students’ writing skill, especially dictation.

  4. Al-Ḥaqīqah al-Muwāfaqah li al-Sharī‘ah: al-Taṣāluḥ bayn al-Taṣawuf wa al-Sharī‘ah bi Nusantara fi al-Qarn al-Sādis ‘Ashr al-Mīlādī

    OpenAIRE

    Iin Suryaningsih

    2013-01-01

    This article discusses the reinterpretation of waḥdat al-wujūd doctrine among Muslims of the archipelago, using the sharī‘ah approach in Sufism as suggested by Al-Būrhanpūrī (d.1620) in his work al-Haqīqah al-Muwāfaqah li al-Sharī‘ah. This work is one of the commentaries (sharḥ) from al-Tuḥfah al-Mursalah ila rūḥ al-Nabī SAW, that invited much debate among Muslims of the archipelago. Al-Būrhanpūrī’s detailed interpretation of waḥdat al-wujūd doctrine proved that the essence of the concep...

  5. Al-Anon and recovery.

    Science.gov (United States)

    Cermak, T L

    1989-01-01

    The history of Al-Anon and its current demographics are reviewed. In order to understand 12-step recovery and psychotherapy for family members of alcoholics, the concept of codependence is defined with a set of diagnostic criteria consistent with the DSM-III-R definition of personality traits and disorders. At the core of codependence are denial and an unrealistic relationship to willpower. The therapeutic implications of considering codependence as a personality disorder are explored, as are the characteristics that make codependence unique among personality disorders: the central role of denial and the existence of a self-help organization to facilitate recovery. The dynamics of working the 12 steps on codependent characteristics are outlined. A synergistic relationship between psychotherapy and the 12 steps is described. Special attention is given the emergence of Al-Anon adult children of alcoholic meetings, and the future of codependence is discussed.

  6. Relativity and Al^+ Optical Clocks

    Science.gov (United States)

    Chou, Chin-Wen; Hume, David B.; Wineland, David J.; Rosenband, Till

    2010-03-01

    We have constructed an optical clock based on quantum logic spectroscopy of an Al+ ion that has a fractional frequency inaccuracy of 8.6x10-18. The frequency of the ^1S0^3P0 clock transition is compared to that of a previously constructed Al^+ optical clock with a statistical measurement uncertainty of 7.0x10-18. The two clocks exhibit a relative stability of 2.8x10-15&-1/2circ;, and a fractional frequency difference of -1.8x10-17, consistent with the accuracy limit of the older clock. By comparing the frequencies of the clocks, we have observed relativistic effects, such as time dilation due to velocities less than 10 m/s and the gravitational red shift from a 0.33 m height change of one of the clocks.

  7. Wear behaviour of Al 261

    Directory of Open Access Journals (Sweden)

    N. Mathan Kumar

    2016-03-01

    Full Text Available Al 2618 matrix material was mixed with the Silicon Nitride (Si3N4, Aluminium Nitride (AlN and Zirconium Boride (ZrB2 reinforced particles. AMC was synthesized successfully by the stir casting method with the various X-wt.% of reinforcements (X = 0,2,4,6,8. Tribological behaviour was studied in this composite with various temperature conditions. The working conditions were Temperature (°C, Load (N, Velocity (m/s and Sliding Distances (m. Before wear testing the mechanical behaviour has been analysed. EDAX was confirmed by the matrix material composition. The Al 2618 alloy and the reinforcement mixers were confirmed by the X-ray Diffraction analysis. Wear rate (mm3/m, Wear resistance (m/mm3, Specific Wear rate (m/Nm and Co-efficient of friction (μ were analysed with various conditions. The worn surfaces were analysed before and after wear testing by Scanning Electron Microscope (SEM. Influence of process parameters and Percentage of contribution were analysed by Taguchi and Analysis of Variance (ANOVA methods. Genetic Algorithm (GA was adopted for optimizing the best and mean of the wear rate and to identify the exact influence of input parameters.

  8. Vormundschaftsrecht als Grundlage politischer Herrschaft

    Directory of Open Access Journals (Sweden)

    Arne Duncker

    2006-07-01

    Full Text Available In ihrer beachtlichen und sehr gewissenhaft dokumentierten Arbeit über vormundschaftliche Regentschaften im 16. und 17. Jahrhundert beschreibt Pauline Puppel unter ausführlichem Bezug auf zeitgenössische Literatur und Archivalien sowohl die allgemeine Rechtslage im damaligen Reich als auch die Anwendungsfälle in Hessen, wo zwischen 1500 und 1700 insgesamt viermal eine Landgräfin als Vormünderin des Fürsten zur Regentin ihres Landes wurde. Dies steht exemplarisch für eine bereits in der Frühen Neuzeit mögliche und nicht einmal ganz seltene Form politischer Frauenherrschaft, die bisher oft nicht hinreichend gewürdigt wurde. Puppels Untersuchung ist in zwei Hauptteile gegliedert. Der erste Teil (Das juristische Regelwerk“, S. 34-143 behandelt die zeitgenössischen Rahmenbedingungen der durch Frauen ausgeübten Vormundschaft und Regentschaft. Der zweite Abschnitt („Die Landgräfinnen von Hessen als Regentinnen“, S. 144-307 befasst sich mit der Ausfüllung dieser Regeln durch konkrete Fallbeispiele in Gestalt von Leben und Regentschaft der regierenden Landgräfinnen von Hessen.

  9. Al Margen de las Cooperativas

    Directory of Open Access Journals (Sweden)

    Gutiérrez Estrada Julio

    1941-04-01

    Full Text Available El sistema Cooperativista, propiamente hablando, no viene a constituir una doctrina económica, es más bien una práctica, un modo de proceder en provecho de determinada agrupación de individuos que se asocian para defenderse de la usura del comercio y del intermediarismo parasitario. "El movimiento cooperativo es tan viejo como la revolución industrial, y ha crecido paralelamente a ella, podría decirse como una protesta en contra del sistema capitalista industrial. Generalmente se considera que tres principios fundamentales determinan su carácter distintivo. 1-Se esfuerza por dar "servido al costo" a sus miembros antes que beneficiarse del negocio con el público en general. El servicio de un grupo organizado se considera como un medio para obtener el máximum de eficacia con mínimo de desperdicio. 2-Insiste en que los beneficios pecuniarios se distribuyan al miembro como productor, después de deducir solamente el interés del capital al tipo que compite, y los salarios y jornales de los trabajadores. 3-Mantiene el control democrático del negocio por todos los participantes en él".

  10. Namadhij Min al-Fikr al-Mali fi al-Islam: Waraqah Ma’lumat Asasiyyah

    Directory of Open Access Journals (Sweden)

    Mustafa Dasuki Kasabah

    2009-03-01

    Full Text Available The renaissance of Islam in various countries has become new phenomenon in the global economy. At the same time it also become the challenge for the Western capitalism and Eastern socialism. This resurrection together with revised ideology and economic system is considered as the strategic solution. Frankly, Islam is the religion which has brought back the core of economy to its position proportionally. In order to get concrete and clear idea on Islamic economy, here the writer presents five books written by Muslim scholars, classic as well as contemporary. The first is Kitab al-Amwal written by Abu Ubaid bin Salam, according to Khalil Harras this book is considered as the best and the most comprehensive book of Islamic economy. The second is Kitab Al-Ahkam al-Sultaniyyah written by Imam Mawardi. The third is Al-Fikr al-Mali written by Ibnu Khaldun, which has high scientific authority from other books, it is systematic as well as contextual. The fourth is al-Iradat al-‘Ammah li al-Daulah al-Islamiyyah fi Sadr al-Islam wa Tatbiqatuha al-Mu’asirah written by Monzer Kahf. The fifth is Al-Nafaqat al-‘Ammah fi Al-Islam: Dirasah Muqaranah written by Yusuf Ibrahim Yusuf. The first three authors are classics and the rest are contemporary.

  11. 77 FR 73732 - In the Matter of Amendment of the Designation of al-Qa'ida in Iraq, aka Jam'at al Tawhid wa'al...

    Science.gov (United States)

    2012-12-11

    ... Designation of al-Qa'ida in Iraq, aka Jam'at al Tawhid wa'al-Jihad, aka The Monotheism and Jihad Group, aka The al-Zarqawi Network, aka al-Tawhid, aka Tanzim Qa`idat al-Jihad fi Bilad al-Rafidayn, aka The Organization of al-Jihad's Base of Operations in Iraq, aka al-Qaida of Jihad in Iraq, aka al-Qaida in Iraq,...

  12. Armaflex in Burj al Arab; Armaflex im Burj al Arab

    Energy Technology Data Exchange (ETDEWEB)

    Steiner, B.

    2003-07-01

    This article describes the Burj al Arab in Dubai, the unique seven-star high-rise hotel. The construction of the hotel, that is built on 250 piles driven into the sand of an artificial island in the Persian Gulf, is described. In particular, various special materials used in the building are described, as are the luxurious fittings and furnishings of the hotel. The cooling and air-conditioning installations that are necessary as a result of the desert climate of Dubai are described and the use of 'Armaflex' elastomer insulating material for the insulation of refrigeration machines and cold-water piping is discussed.

  13. Microstructure of interaction interface between Al-Si, Zn-Al alloys and Al2O3p/6061Al composite

    Institute of Scientific and Technical Information of China (English)

    许志武; 闫久春; 吕世雄; 杨士勤

    2004-01-01

    Interaction behaviors between Al-Si, Zn-AI alloys and Al2O3p/6061AI composite at different heating temperatures were investigated. It is found that Al2O3p/6061Al composite can be wetted well by AlSi-1, AlSi-4 and Zn-Al alloys and an interaction layer forms between the alloy and composite during interaction. Little Al-Si alloys remain on the surface when they fully wet the composite and Si element in Al-Si alloy diffuses into composite entirely and assembles in the composite near the interface of Al-Si alloy/composite to form a Si-rich zone. The microstructure in interaction layer with Si penetration is still dense. Much more residual Zn-Al alloy exists on the surface of composite when it wets the composite, and porosities appear at the interface of Zn-Al alloy/composite. The penetration of elements Zn, Cu of Zn-Al alloy into composite leads to the generation of shrinkage cavities in the interaction layer and makes the microstructure of Al2 O3p/6061A1 composite loose.

  14. Investigations of Al-Dalang and Al-Hawashat meteorites

    Science.gov (United States)

    Gismelseed, A. M.; Abdallah, S. B.; Al-Rawas, A. D.; Al-Mabsali, F. N.; Widatallah, H. M.; Elzain, M. E.; Yousif, A. A.; Ericsson, T.; Annersten, H.

    2016-12-01

    Mössbauer spectroscopy, X-ray diffraction (XRD) measurements, and electron microprobe analysis (EMPA) have been performed on two meteorites named Al-Dalang and Al-Hawashat after identifying their falling sites in the Western region of Sudan. These two meteorites are ordinary chondrites with similar mineralogy. XRD and EMPA show that the two specimens consist of primary olivine, ortho-pyroxene and later crystallising clino-pyroxene as reaction rims against plagioclase. Fe-metal phases are dominated by kamacite (≈6 wt.% Ni) and minor amounts of tetrataenite (≈52 wt.% Ni). Troilite (FeS) and alabandite (MnS) are optically observed as sulphide phases. The Mössbauer measurements at 295 and 78 K are in agreement with the above characterizations, showing at least two paramagnetic doublets which are assigned to olivine and pyroxene and magnetic sextets assigned to kamacite (hyperfine field ≈33.5 T) and troilite FeS (hyperfine field ≈31 T).

  15. Electrical conductivity of (Na3AlF6-40%K3AlF6)-AlF3-Al2O3 melts

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    The effects of contents of AlF3 and Al2O3,and temperature on electrical conductivity of (Na3AlF6-40%K3AlF6)-AlF3-Al2O3 were studied by continuously varying cell constant (CVCC) technique.The results show that the conductivities of melts increase with the increase of temperature,but by different extents.Every increasing 10 ℃ results in an increase of 1.85×10-2,1.86×10-2,1.89×10-2 and 2.20×10-2 S/cm in conductivity for the (Na3AlF6-40%K3AlF6)-AlF3 melts containing 0%,20%,24%,and 30% AlF3,respectively.An increase of every 10 ℃ in temperature results an increase about 1.89×10-2,1.94×10-2,1.95×10-2,1.99×10-2 and 2.10×10-2 S/cm for (Na3AlF6-40%K3AlF6)-AlF3-Al2O3 melts containing 0%,1%,2%,3% and 4% A12O3,respectively.The activation energy of conductance was calculated based on Arrhenius equation.Every increasing 1% of AlF3 results in a decrease of 0.019 and 0.020 S/cm in conductivity for (Na3AlF6-40%K3AlF6)-AIF3 melts at 900 and 1 000 ℃,respectively.Every increase of 1% Al2O3 results in a decrease of 0.07 S/cm in conductivity for (Na3AlF6-40%K3AlF6)-AlF3-Al2O3 melts.The activation energy of conductance increases with the increase in content of AlF3 and Al2O3.

  16. United modification of Al-24Si alloy by Al-P and Al-Ti-C master alloys

    Institute of Scientific and Technical Information of China (English)

    韩延峰; 刘相法; 王海梅; 王振卿; 边秀房; 张均艳

    2003-01-01

    The modification effect of a new type of Al-P master alloy on Al-24Si alloys was investigated. It is foundthat excellent modification effect can be obtained by the addition of this new type of A1-P master alloy into Al-24Simelt and the average primary Si grain size is decreased below 47 μm from original 225 μm. It is also found that theTiC particles in the melt coming from Al8Ti2C can improve the modification effect of the Al-P master alloy. Whenthe content of TiC particles in the Al-24Si melt is 0.03 %, the improvement reaches the maximum and keeps steadywith increasing content of TiC particles. Modification effect occurs at 50 min after the addition of the Al-P master al-loy and TiC particles, and keeps stable with prolonging holding time.

  17. Kurzwortbildungen als Merkmal der Deutschenrechtssprache

    Directory of Open Access Journals (Sweden)

    Ljubica Kordić

    2013-12-01

    Full Text Available In deutscher Rechtssprache kommen häufig die Nominalkomposita vor, die auf eineim Vergleich mit der Gemeinsprache ungewöhnliche Weise gebildet werden. Diese Wortbildungsform wird in deutschen Grammatiken unterschiedlich bestimmt: als Reduktion, Kurzwortbildung, Kontraktion bzw. Klammerform. Dieser Wortbildungstypkann mit dem Kompositum Mietblock illustriert werden, das durch Verkürzung aus Miet(wohnungsblock entstanden ist. Im Kroatischen, wo Komposition als Wortbildungstyp relativ schwach vertreten ist, ist die Bildung solcher Komposita theoretisch nur angedeutet. Da die Komposita dieses Bildungstyps in deutschen Rechtstexten stark vertreten sind, ist das Ziel dieses Beitrags, auf ihre Gebräuchlichkeit als ein für deutsche Rechtstexte charakteristisches Merkmal hinzuweisen. In der Einleitung befasstsich die Autorin mit der Begriffsbestimmung, der theoretischen Determinierung undder Abgrenzung dieser Kompositionsart von anderen Sonderfällen. Den Hauptteil der Arbeit bildet die Untersuchung der Kurzwortbildungen (Klammerformen, Reduktionen, Verschmelzungen, die dem geltenden deutschen Strafgesetzbuch von 2009 entnommensind, und ihrer kroatischen Entsprechungen. Der Beitrag hat die Aufgabe, auf Grund der gesammelten Belege die Häufigkeit und die Bildungsmuster der Komposita dieses Typs in deutscher Rechtssprache zu bestimmen und durch semantische Transformationsmuster ihren Informationswert in Bezug auf die Konstituenten festzustellen, aus denen sie gebildet wurden. Es wird auch untersucht, welche Bildungstypen ihrekroatischen Äquivalente aufweisen und ob es irgendwelche Unterschiede in ihrem informativen Wert gibt. Die Autorin bedient sich der Methode der Datensammlung, der qualitativen semantischen Analyse der Belege nach den Transformationsmodellen von Fleischer und Barz und der Synthese der Resultate. Die Resultate dieser Untersuchung sollten einen Beitrag zur Erforschung der der Rechtslinguistik im allgemeinen leisten, inbesondere im

  18. Reply to Pachai et al.

    Science.gov (United States)

    Harrison, William J; Bex, Peter J

    2016-05-01

    Peripheral vision is fundamentally limited by the spacing between objects. When asked to report a target's identity, observers make erroneous reports that sometimes match the identity of a nearby distractor and sometimes match a combination of target and distractor features. The classification of these errors has previously been used to support competing 'substitution' [1] or 'averaging' [2] models of the phenomenon known as 'visual crowding'. We recently proposed a single model in which both classes of error occur because observers make their reports by sampling from a biologically-plausible population of weighted responses within a region of space around the target [3]. It is critical to note that there is no probabilistic substitution or averaging process in our model; instead, we argue that neither substitution nor averaging occur, but that these are misclassifications of the distribution of reports that emerge when a population response distribution is sampled. This is a fundamentally different way of thinking about crowding, and on this basis we claim to have provided a mechanism unifying categorically distinct perceptual errors. Our goal was not to model all crowding phenomena, such as the release from crowding when target and flanks differ in color or depth [4]. Pachai et al.[5] have suggested that our model is not unifying because it inaccurately predicts perceptual performance for a particular stimulus. Although we agree that our model does not predict their data, this specific demonstration overlooks the critical aspect of the model: perceptual reports are drawn from a weighted population code. We show that Pachai et al.'s [5] own data actually provide evidence for the population code we have described [3], and we suggest a biologically-plausible analysis of their stimuli that provides a computational basis for their 'grouping' account of crowding.

  19. A melancolia em Al Berto

    OpenAIRE

    Camila Emanuele Martins de Souza

    2015-01-01

    Este trabalho objetiva estudar, sob a perspectiva da melancolia, os livros Salsugem (1984) e Horto de Incêndio (1997), de Al Berto. O autor português produz escritos em que a temática da melancolia atravessa a palavra, o que justifica o debruçar-se sobre as construções textuais da poética albertiana, a fim de traçar-lhe novas possibilidades de leitura por esse ângulo. Para isso, com o intuito de compreender o discurso melancólico segue-se o embasamento teórico: de Klibansky, Panosfsky e Saxl ...

  20. Alí Caro, alarife

    OpenAIRE

    2009-01-01

    Quizá el más sobresaliente sea el de Alí Caro, un alarife que a finales del siglo xv proyectó y dirigió la construcción del castillo de Coca, la obra más insigne de la arquitectura militar mudéjar del siglo xv castellano. Efectivamente, encontramos que hacia 1486 fue reclamado por don Alonso FonsecaAvellaneda, señor de las villas de Coca y Alaejos, para levantar un impresionante castillo -que, a la vez, cumpliría las funciones de palacio nobiliar- en Coca, con la ayuda de sus hermanos Yuc;...

  1. La heredad junto al llanto

    Directory of Open Access Journals (Sweden)

    Javier Arias Ramírez

    1962-11-01

    Full Text Available En "La heredad junto al llanto", Carlos Palacio La verde canta todo lo elemental y simple con sobriedad y elegancia que dan mérito a su poesía. Por eso consideramos un acierto de sus editores el divulgar la obra de este joven poeta, que como dice en su introducción René Uribe Ferrer, no es conocido porque ha rehuido casi sistemáticamente la publicidad, lo cual me asegura que en este proceder hay una grande honestidad intelectual, una verdadera personalidad poética.

  2. Personalidad y tolerancia al dolor

    OpenAIRE

    JESÚS M.ª CARRILLO; SUSANA COLLADO; NIEVES ROJO

    2005-01-01

    Se examina la relación de algunas variables de personalidad -EPQ-A (Eysenck y Eysenck, 1975), NEO-PI (McCrae y Costa, 1988) y STA -personalidad esquizotípica- y STB -personalidad límite-, de Claridge y Broks (1984), con la tolerancia al dolor experimental inducido mediante agua fría (cold pressor test), según el procedimiento de Staats, Heckmat y Staats (1998) en una muestra filtrada mediante un cuestionario sobre dolor crónico, dolores de cabeza, de espalda, artritis, síndrome de Raynaud, y ...

  3. Gettysburg: Monumento al mundo libre

    Directory of Open Access Journals (Sweden)

    Neutra, Richard J.

    1961-12-01

    Full Text Available En el verano del año 1958 fui invitado, por las autoridades de la Universidad de Arizona, en Tucson, a la colocación de la primera piedra de su futuro taller, construido para favorecer el desarrollo técnico del Estado. Varios alcaldes y ministros presenciaron la ceremonia, que fue muy interesante, y uno de los periodistas presentes, John Riddick, del "Tucson Daily Citizen", invitó cordialmente, a mi esposa y a mí, a atravesar, al día siguiente, la frontera mejicana, que cruzaba un vasto desierto.

  4. Al-Ḥaqīqah al-Muwāfaqah li al-Sharī‘ah: al-Taṣāluḥ bayn al-Taṣawuf wa al-Sharī‘ah bi Nusantara fi al-Qarn al-Sādis ‘Ashr al-Mīlādī

    Directory of Open Access Journals (Sweden)

    Iin Suryaningsih

    2013-04-01

    Full Text Available This article discusses the reinterpretation of waḥdat al-wujūd doctrine among Muslims of the archipelago, using the sharī‘ah approach in Sufism as suggested by Al-Būrhanpūrī (d.1620 in his work al-Haqīqah al-Muwāfaqah li al-Sharī‘ah. This work is one of the commentaries (sharḥ from al-Tuḥfah al-Mursalah ila rūḥ al-Nabī SAW, that invited much debate among Muslims of the archipelago. Al-Būrhanpūrī’s detailed interpretation of waḥdat al-wujūd doctrine proved that the essence of the concept did not contradict the basic principles of Islam as contained in the Quran and Hadith. Al-Būrhanpūrī  interpreted the concept through explanations that adjusted to the audience’s competence, utilizing oral language carefully in revealing spiritual experience not easily explained, as well as employing the bases of sharī‘ah. Al-Haqīqah al-Muwāfaqah has provided the information about the explanations of interpretation tradition among the archipelago sufis.Copyright (c 2014 by SDI. All right reserved.DOI: 10.15408/sdi.v20i1.350 

  5. Betaalde arbeid als verdringer van vrijwilligerswerk? Overbelasting als mogelijke verklaring

    Directory of Open Access Journals (Sweden)

    Angela Kok-Van Meer

    2014-06-01

    Full Text Available Do paid work activities displace volunteering? A potential explanation from role overload theoryRecent changes in Dutch society might lead to a shortage of volunteers. On the one hand, the demand for volunteers is increasing: in the social welfare domain because of governmental austerity policies and in other domains such as sports because of the higher supervision intensity of activities. On the other hand, the supply of volunteers is likely to decrease as more and more women and older workers are active in the labor market, which reduces the time that is available for volunteering. Role overload theory would suggest that the increasing labor participation rate of certain categories of workers goes along with a decline of participation in voluntary work. The secondary data analysis shows that various role loads (work, care for children living at home are associated with differences in participation in voluntary work. However, while a higher number of contractual work hours and hours spent on work-related training are negatively associated with volunteer work, care for children living at home is positively associated with volunteering. These results provide only limited support for role overload theory.Betaalde arbeid als verdringer van vrijwilligerswerk? Overbelasting als mogelijke verklaringDe afgelopen jaren hebben zich enkele maatschappelijke veranderingen voorgedaan, die wellicht tot schaarste op de Nederlandse vrijwilligersmarkt zouden kunnen leiden. Enerzijds neemt de vraag naar vrijwilligers toe doordat subsidieregelingen versoberen (bijvoorbeeld in zorg en welzijn en de begeleidingsintensiteit in bepaalde organisaties stijgt (bijvoorbeeld in sport. Anderzijds neemt het aanbod van vrijwilligers af doordat vrouwen en ouderen meer en langer op de arbeidsmarkt actief zijn, waardoor minder tijd beschikbaar is voor vrijwilligerswerk. In dit artikel wordt aan de hand van de role overload theorie nagegaan of het al dan niet deelnemen aan

  6. Ti_3AlC_2-Al_2O_3-TiAl_3 composite fabricated by reactive melt infiltration

    Institute of Scientific and Technical Information of China (English)

    HE Shan-shan; YIN Xiao-wei; ZHANG Li-tong; LI Xiang-ming; CHENG Lai-fei

    2009-01-01

    Porous preforms were fabricated by cold-pressing process using powder mixture of TiC, TiO_2 and dextrin. After pyrolysis and sintering, Al melt was infiltrated into the porous preforms, leading to the formation of Ti_3AlC_2-Al_2O_3-TiAl_3 composite. Effects of cold-pressing pressure of preforms on microstructures and mechanical properties of the composites were studied. Synthesis mechanism and toughening mechanism of composite were also analyzed. The results shows that TiO_2 is reduced into Ti_2O_3 by carbon, the decomposition product of dextrin, which causes the spontaneous infiltration of Al melt into TiC/Ti_2O_3 preform. Then, Ti_3AlC_2-Al_2O_3-TiAl_3 composite is in-situ formed from the simultaneous reaction of Al melt with TiC and Ti_2O_3. With the increase of cold-pressing pressure from 10 MPa to 40 MPa, the pore size distribution of the preforms becomes increasingly uniform after pre-sintering, which results in the reduction of defects, and the decrease of property discrepancy of composites. Nano-laminated Ti_3AlC_2 grains and Al_2O_3 particles make the fracture toughness of TiAl_3 increase remarkably by various toughening mechanisms including stress-induced microcrack, crack deflection and crack bridging.

  7. Ce对Al-Si-Cu合金中α(Al)-Al2Cu共晶形貌的影响%Effect of Ce on morphology ofα(Al)-Al2Cu eutectic in Al-Si-Cu alloy

    Institute of Scientific and Technical Information of China (English)

    Maja VONINA; Joef MEDVED; Tonica BONINA; Franc ZUPANI

    2014-01-01

    The effect of Ce addition on the morphology of the α(Al)-Al2Cu eutectic in Al-Si-Cu alloy was investigated using thermal analysis, light microscopy, scanning electron microscopy, focused ion beam and energy dispersive analysis. The results show that the eutectic α(Al)-Al2Cu forms within small space between dendrites, silicon and AlSiFeMn plates. Eutectic Al2Cu is not lamellar but degenerated. However, Al2Cu in Ce-modified alloys is more compact. Ce partially dissolves in Al2Cu, which is a viable reason for the formation of coarser Al2Cu. The addition of Ce also increases the microhardness of theα(Al)-Al2Cu eutectic by almost 10%compared with the basic Al-Si-Cu alloy.%采用热分析、光学显微镜技术、扫描电镜技术、聚焦离子束和能量色散谱分析方法研究Ce对Al-Si-Cu合金中α(Al)-Al2Cu共晶形貌的影响。结果表明,在枝晶、硅和AlSiFeMn之间较小空间内形成了α(Al)-Al2Cu共晶。Al2Cu为非层状的不规则共晶组织。Al2Cu在经Ce改性的合金中更加致密。部分Ce溶解于Al2Cu中,这是粗晶Al2Cu形成的原因。与基体Al-Si-Cu合金相比,Ce的加入能使α(Al)-Al2Cu共晶合金的显微硬度提高约10%。

  8. con bajo peso al nacer

    Directory of Open Access Journals (Sweden)

    Adriana Mora Antó

    2005-01-01

    Full Text Available Esta investigación dio cuenta de la relación entre el estilo de funcionamiento familiar, los patrones de crianza y las edades de desarrollo evolutivo en niños, nacidos con bajo peso. El estudio descriptivo correlacional se realizó con 41 niños y sus madres, aplicándose cuestionarios sobre funcionamiento familiar, prácticas de crianza y desarrollo infantil. Los resultados señalaron la existencia de un funcionamiento familiar caracterizado por una cohesión amalgamada y una adaptabilidad caótica, una disciplina complaciente, falta de control y de límites claros en la díada madre-hijo. Se trataba de familias monoparentales, donde la temprana edad de concepción, el madresolterismo y el apoyo de la familia extensa eran constantes. Las edades evolutivas registradas indicaron un desarrollo inferior a la edad cronológica, en la mayor parte de los casos; sin embargo, éstas tendieron a ser superiores al compararlas con la edades reales de los infantes. No se encontró una correlación estadísticamente significativa entre la edad de desarrollo y los diferentes factores del funcionamiento familiar para algunos de los rangos de edad considerados; sin embargo, no se lo descartó por completo, especialmente en lo referente al optimismo familiar

  9. Everyday Life with ALS: A Practical Guide

    Science.gov (United States)

    ... the wheelchair plus its user, and more. Van shoppers can buy from a mobility dealer or directly ... Life with ALS: A Practical Guide 65 Connect Online with the ALS Community! MDA’s free online community — ...

  10. Formaline of alternatieven als ontsmettingsmiddel voor broedeieren

    NARCIS (Netherlands)

    Meijerhof, R.; Dijk, D.

    1997-01-01

    Formaline wordt algemeen gezien als een goed middel om broedeieren te ontsmetten. Toch geeft ontsmetting met middelen als ozon en waterstofperoxyde vaak een iets beter broedresultaat. Een eventuele verklaring hiervoor is momenteel nog niet te geven.

  11. Cenomanian-Turonian biostratigraphy of the Jardas Al Abid area, Al Jabal Al Akhdar, northeast Libya

    Science.gov (United States)

    El Qot, Gamal M.; Abdulsamad, Esam O.

    2016-09-01

    The Upper Cenomanian-Turonian succession exposed at Jardas al'Abid area consists mainly of carbonates with siliciclastic intercalations. This succession is subdivided lithostratigraphically into: Qasr al'Abid (Late Cenomanian) and Al Baniyah (Late Cenomanian-Coniacian) formations. This sequence is relatively rich in macrofossil assemblages especially bivalves, gastropods, and echinoids with rare ammonites. Based on the first occurrence (FO) and last occurrence (LO) of some index species of these macrofossil groups, an integrated biostratigraphic framework has been constructed. The studied Cenomanian-Turonian sequence is subdivided biostratigraphically into three ammonite biozones; Pseudaspidoceras pseudonodosoides Total Range Zone, Choffaticeras segne Total Range Zone, and Coilopoceras requienianum Total Range Zone. Based on the rest of macrofossil assemblages other than the ammonites, eight biozones were recognized; Mecaster batnensis Total Range Zone, Ceratostreon flabellatum-Neithea dutrugei Acme Zone, Costagyra olisiponensis Acme Zone, Pycnodonte (Phygraea) vesicularis vesiculosa Acme Zone, Mytiloides labiatus Total Range Zone = Mecaster turonensis Acme Zone, Rachiosoma rectilineatum-Curvostrea rouvillei-Tylostoma (T.) globosum Assemblage Zone, Radiolites sp.-Apricardia? matheroni Total Range Zone, and Nerinea requieniana Total Range Zone. Most of the proposed biozones are recorded for the first time from Libya. The integration among these biozones as well as local and inter-regional correlation of the biozones have been discussed. The stage boundaries of the studied stratigraphic intervals are discussed, where the Cenomanian/Turonian boundary is delineated at the last occurrence (LO) of Pseudaspidoceras pseudonodosoides (Choffat), while the Turonian/Coniacian boundary is delineated arbitrary being agree with the LO of the Turonian fauna.

  12. Grainreifnementlimit of commercialpureAl inoculatedbyAl-Ti-C(B)masteralloys

    Institute of Scientific and Technical Information of China (English)

    En-zhao Wang; Xiang-fa Liu

    2015-01-01

    The grain reifnement limits of commercial pure Al inoculated by Al-5Ti-1B, Al-5Ti-0.25C and Al-5Ti-0.3C-0.2B master al oys were studied, and the inlfuence of melting temperature on the grain reifning performance of these three master al oys was investigated using a high scope video microscope (HSVM), a ifeld-emission scanning electron microscope (FESEM), an electron probe micro-analyzer (EPMA) and X–ray diffraction (XRD) method. Results show that there is a grain reifnement limit of commercial pure Al reifned by these three master al oys; with the same addition level of 1.5% under the present experimental conditions, the grain reifnement limits (smal est average grain size) of commercial pure Al reifned by Al-5Ti-1B, Al-5Ti-0.25C and Al-5Ti-0.3C-0.2B master al oys are 50 µm, 80 µm and 80 µm, respectively. In addition, with an increase in the melting temperature of the pure Al, the grain reifning performance of Al-5Ti-1B and Al-5Ti-0.25C master al oys decreases, but the grain reifning performance of Al-5Ti-0.3C-0.2B changes little.

  13. Framework Al zoning in zeolite ECR-1.

    Science.gov (United States)

    Shin, Jiho; Ahn, Nak Ho; Cho, Sung June; Ren, Limin; Xiao, Feng-Shou; Hong, Suk Bong

    2014-02-25

    Rietveld analyses of the synchrotron X-ray diffraction data for various cation forms of zeolite ECR-1 have demonstrated framework Al zoning, which parallels the alternation of Al-rich maz and Al-poor mor layers. This can be further supported by notable differences in the average bond valence of its 10 crystallographically distinct tetrahedral sites.

  14. Electroplating Zn-Al Alloy Technology

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    The method of controlling separating anode and separating power source was used to perform orthogonal optimization for the parameters in electroplating Zn-Al alloy.The electroplating Zn-Al alloy technology was decided, in which the content of Al is about 12%-15%.

  15. Efeito do cloreto de cálcio e do tratamento hidrotérmico na atividade enzimática e no teor de fenólicos do abacaxi Effect of calcium chloride and hydrothermical treatment on enzymatic activity and phenol content of pineapple

    Directory of Open Access Journals (Sweden)

    NEIDE BOTREL GONÇALVES

    2000-10-01

    Full Text Available O abacaxi (Ananas comosus Mill. está sujeito a danos causados pelo frio durante o armazenamento refrigerado. A aplicação, pós-colheita, de Ca pode contribuir para reduzir vários tipos de desordens fisiológicas. Neste trabalho, verificou-se a influência da aplicação, pós-colheita, de CaCl2 a 2%, associada ao tratamento hidrotérmico (38ºC e 40ºC por 10 e 20 minutos de imersão, na composição química (fenólicos e enzimas, e na suscetibilidade ao escurecimento interno do abacaxi (Ananas comosus Mill. cultivar Smooth Cayenne. Os frutos foram armazenados a 9ºC e umidade relativa de 90% por um período de 15 dias. As avaliações foram efetuadas sete dias após a retirada dos frutos das condições de refrigeração. O tratamento dos frutos com CaCl2 reduziu o índice de escurecimento interno, conferindo menor atividade das enzimas polifenoloxidase, peroxidase e fenilalanina amônio liase e reduzindo o teor de compostos fenólicos na polpa quando associado ao tratamento hidrotérmico, independentemente do tempo de imersão.Pineapple (Ananas comosus Mill. is exposed to injuries caused by low temperature during the refrigerated storage. The post-harvest application of Ca may contribute to reduce a number of physiological disorders. In this work, the influence of the postharvest application of 2% CaCl2 associated with hydrothermical treatment (38ºC and 40ºC for 10 and 20 minutes, on the chemical composition (enzymes, phenols, and susceptibility to internal browning of pineapple `Smooth Cayenne' fruits was studied. The fruits were stored at 9ºC and 90% relative humidity for 15 days. The evaluations were performed seven days after removal of the fruits from the refrigerated conditions. The immersion of the fruits into a 2% CaCl2 solution reduced the index of internal browning and diminished the activities of the enzymes polyphenoloxidase, peroxidase and phenilalanine ammonium lyase, as well as the content of phenolic compounds when

  16. Transport Properties of the Tunnel Junctions Al1-xCox/{Al1-xCox-oxide}/Al

    Institute of Scientific and Technical Information of China (English)

    X.F.Han; J. Murai; H.Kubota; Y.Ando; Q.Y.Wang; N.Tezuka; T.Miyazaki

    2000-01-01

    The oxides of Al1-xCox (x=0,0.25,0.5,0.75, and 1.0) alloys were chosen as barrier materials in this work. The tunnel junction consists of the bottom electrode Al1-xCox and the top electrode Al with an insulating layer { Al1-xCox-oxide} which was formed by natural oxidation in a baking-box at 333K. The oxidation time for forming an Al1-xCox-oxide layer on the surface of the bottom Al1-xCox layers were optimized.The resistance of Al1-xCox/{ Al1-xCox-oxide}/Al tunnel junctions varied between 101 and 106 Ω measured at 1 my and 4.2 K. The effective barrier height and width of insulating layers Al1-xCox-oxide ( x=0.25, 0.5, and 0.75 )varied between 0.6 and 2.7 eV and between 1.3 and 2.1 nm. It is shown that the thin oxide layer of Al1-xCox alloys can be chosen as barrier materials.

  17. Oxidation protection of NiCoCrAlY coatings on γ-TiAl

    Institute of Scientific and Technical Information of China (English)

    WANG Qi-min; GUO Ming-hu; KE Pei-ling; GONG Jun; SUN Chao; WEN Li-shi

    2005-01-01

    The effect of NiCoCrAlY overlay coatings on the oxidation resistance of γ-TiAl was studied at 900 ℃ in static air. To hinder the interdiffusion of the elements, the Al/Al2O3 layer was added between the coating and the alloy. The results show that the TiAl alloy exhibits poor oxidation resistance. NiCoCrAlY coating can not effectively protect the γ-TiAl substrate from high temperature oxidation because of the serious interdiffusion between the coating and the substrates. With Al/Al2O3 diffusion barrier, the NiCoCrAlY coating exhibits excellent oxidation protection on γ-TiAl alloy.

  18. Trato verbal paterno al adolescente

    Directory of Open Access Journals (Sweden)

    Rodríguez Juan Carlos

    1995-06-01

    Full Text Available

    En alguna ocasión se ha escuchado una palabra, que causa un sentimiento y a la vez un recuerdo, que lleva a evocar la adolescencia o la infancia, se recuerda quien la pronunciaba y en que ocasión la decía. Este es el poder que tiene una palabra y más aún si es dicha por el padre, puesto que este es la figura significativa que se lleva en la memoria. De aquí nace el interés de realizar un estudio, en donde se describe y analice la percepción y el sentimiento del adolescente, quien en esta etapa es vulnerable al cambio, ya que está buscando su propia identidad; que con el trato verbal paterno la encontrara sin ninguna dificultad o por el contrario nunca la encontrará.

     

  19. Entscheidung als Häresie

    Directory of Open Access Journals (Sweden)

    Jaanus Sooväli

    2014-06-01

    Full Text Available Man könnte sagen, dass Aristoteles so etwas wie eine traditionelle und dominierende Auffassung der Entscheidung in der westlichen Philosophie gründet. Das Ziel dieses Aufsatzes ist es, diese Auffassung von Aristoteles zu entwerfen, einige ihrer wichtigsten Probleme anzusprechen, und sie damit zu kontrastieren, was ich Jacques Derridas häretische „Konzeptualisierung“ der Entscheidung nennen würde. Es wird gezeigt, dass diese häretische Thematisierung von Derrida nicht nur und einfach mit dem traditionellen aristotelischen Begriff bricht, sondern vielmehr auch zu Recht einige seiner wichtigsten Züge auf solcher Weise schärft, dass von diesem radikalisierten Standpunkt die Behandlung von Aristoteles als die Annullierung der Entscheidung zum Vorschein kommt. Es wird zudem impliziert, wie Derridas häretische Auffassung die Erneuerung der Ethik und Politik mit sich bringt.

  20. Instandhaltungsmanagement als Gestaltungsfeld Ganzheitlicher Produktionssysteme

    Science.gov (United States)

    Dombrowski, Uwe; Schulze, Sven; Otano, Isabel Crespo

    Sich kontinuierlich verändernde Rahmenbedingungen, wie beispielsweise eine steigende Variantenvielfalt, verkürzte Produktlebenszyklen sowie Kundenforderungen nach höherer Qualität, kürzeren Lieferzeiten und geringeren Kosten, fordern von produzierenden Unternehmen eine stetige Anpassung der Prozesse, der Organisation und der Strukturen. Seit den 90er Jahren versuchen immer mehr deutsche Unternehmen diesen veränderten Anforderungen mit der Einführung eines Ganzheitlichen Produktionssystems (GPS) zu begegnen. Ganzheitliche Produktionssysteme sind dabei in ihren Grundlagen an das Toyota Produktionssystem angelehnt, vereinigen aber auch weitere Methoden zu einem unternehmensspezifischen Regelwerk. Im Rahmen des langfristigen Trends zu unternehmensindividuellen Produktionssystemen wird sowohl in der Industrie als auch in der Forschung intensiv über das Toyota Produktionssystem, Lean Production, Lean Management und Ganzheitliche Produktionssysteme diskutiert, werden Konzepte zu Implementierung und Betrieb erstellt und die Wirtschaftlichkeit untersucht.

  1. Friction Stir Welding of Al 5052 with Al 6061 Alloys

    Directory of Open Access Journals (Sweden)

    N. T. Kumbhar

    2012-01-01

    Full Text Available Friction stir welding (FSW, a solid-state joining technique, is being extensively used in similar as well as dissimilar joining of Al, Mg, Cu, Ti, and their alloys. In the present study, friction stir welding of two aluminium alloys—AA6061 and AA5052—was carried out at various combinations of tool rotation speeds and tool traverse speeds. The transverse cross-section of the weld was used for optical as well as electron microscopy observations. The microstructural studies were used to get an indication of the extent of material mixing both at the macro- and microscales. It was observed that, at the interface region, both materials exhibited similar texture despite the nonrigorous mixing of the materials in the nugget. The extent of interdiffusion of alloying elements at the interface was studied using electron probe microanalysis. The tensile testing evaluation of these specimens showed good mechanical properties. The interdiffusion of alloying elements and development of similar orientations in the nugget could have contributed to the better tensile properties of the friction-stir-welded AA5052-AA6061 specimens.

  2. Al-Fe-Si、Al-Mn-Si与Al-Fe-Mn-Si体系热力学描述的更新%Update of Al-Fe-Si, Al-Mn-Si and Al-Fe-Mn-Si thermodynamic descriptions

    Institute of Scientific and Technical Information of China (English)

    陈海林; 陈清; 杜勇; Johan BRATBERG; Anders ENGSTR(O)M

    2014-01-01

    采用Calphad方法对Al-Fe-Mn-Si四元系及其子体系进行热力学评估.首先,通过考虑文献中最新的实验研究结果以及对部分三元化合物应用新的热力学模型,修正Al-Fe-Si三系的热力学描述,显著地改善了整个成分范围内、尤其是富Al角的液相面投影图.随后,对三元化合物α-AlMnSi和β-AlMnSi采用新的模型,精修Al-Mn-Si体系富Al角的热力学描述.然后,通过模拟α-AlMnSi相在Al-Fe-Mn-Si体系中的固溶度,优化Al-Fe-Mn-Si四元系富Al角的热力学描述.在优化时,对α-AlMnSi作特殊考虑并加入限制条件,以确保其不会在Al-Fe-Si三元系中变得稳定.最后,将所获得的热力学描述加入TCAL数据库,通过一系列的相平衡计算与凝固模拟、以及与商业铝合金的实验数据的比较,对所获得的热力学描述进行全面的验证.更新后的TCAL数据库能够可靠地预测Al-Fe-Si基与Al-Fe-Mn-Si基合金中的相形成.

  3. Preparation and Characterization of Highly Flexible Al2O3/Al/Al2O3 Hybrid Composite

    Directory of Open Access Journals (Sweden)

    Zhijiang Wang

    2015-01-01

    Full Text Available The natural brittleness of oxide ceramics heavily inhibits their more extensive applications. In present research, a highly flexible Al2O3/Al/Al2O3 hybrid composite was fabricated by employing plasma electrolysis oxidation to in situ grow alumina layers on Al foil, in which an outside layer of nanostructured polycrystalline oxide ceramic was composed of nanosized grains with the size of around 17 nm. Due to shear band formation, nanosized circle bubbles prolonging the crack path, grain rotation, and deformation, the fabricated Al2O3/Al/Al2O3 hybrid composite contains no observable cracks even after being bent on a cylindrical bar with a curvature of 1.5 mm. The composite exhibits alumina stiffness at the elastic stage and aluminum ductility during plastic deformation, which provides high flexibility with the well-integrated properties of the components. In a synergistic interaction, the alumina on the outside exhibited a strain of 0.33% at room temperature, which was higher than optimum value of 0.25% presented by reported most flexible oxide ceramics. With the unique characteristics and properties, the Al2O3/Al/Al2O3 composite demonstrates a great potential for various engineering applications.

  4. Cryptanalysis of Lin et al.'s Efficient Block-Cipher-Based Hash Function

    NARCIS (Netherlands)

    Liu, Bozhong; Gong, Zheng; Chen, Xiaohong; Qiu, Weidong; Zheng, Dong

    2010-01-01

    Hash functions are widely used in authentication. In this paper, the security of Lin et al.'s efficient block-cipher-based hash function is reviewed. By using Joux's multicollisions and Kelsey et al.'s expandable message techniques, we find the scheme is vulnerable to collision, preimage and second

  5. Magnetism of Al-substituted magnetite reduced from Al-hematite

    Science.gov (United States)

    Jiang, Zhaoxia; Liu, Qingsong; Zhao, Xiang; Roberts, Andrew P.; Heslop, David; Barrón, Vidal; Torrent, José

    2016-06-01

    Aluminum-substituted magnetite (Al-magnetite) reduced from Al-substituted hematite or goethite (Al-hematite or Al-goethite) is an environmentally important constituent of magnetically enhanced soils. In order to characterize the magnetic properties of Al-magnetite, two series of Al-magnetite samples were synthesized through reduction of Al-hematite by a mixed gas (80% CO2 and 20% CO) at 395°C for 72 h in a quartz tube furnace. Al-magnetite samples inherited the morphology of their parent Al-hematite samples, but only those transformed from Al-hematite synthesized at low temperature possessed surficial micropores, which originated from the release of structural water during heating. Surface micropores could thus serve as a practical fingerprint of fire or other high-temperature mineralogical alteration processes in natural environments, e.g., shear friction in seismic zones. In addition, Al substitution greatly affects the magnetic properties of Al-magnetite. For example, coercivity (Bc) increases with increasing Al content and then decreases slightly, while the saturation magnetization (Ms), Curie temperature (Tc), and Verwey transition temperature (Tv) all decrease with increasing Al content due to crystal defect formation and dilution of magnetic ions caused by Al incorporation. Moreover, different trends in the correlation between Tc and Bc can be used to discriminate titanomagnetite from Al-magnetite, which is likely to be important in environmental and paleomagnetic studies, particularly in soil.

  6. MARTENSITIC TRANSFORMATION AND THERMAL STABILITY IN Cu-Al-Co AND Cu-Al-Zr ALLOYS

    Institute of Scientific and Technical Information of China (English)

    Y. Q. Ma; C.B. Jiang; H.B. Xu

    2003-01-01

    Cu-Al-Co and Cu-Al-Zr alloys were explored with Co or Zr additions in Cu-Al alloys for high temperature shape memory alloys. Samples were quenched after homogenized at 850℃ for 48h. It was found that both Cu-Al-Co and Cu-Al-Zr show AlCu3 martensitic phase at room temperature and exhibit martensitic transformation temperatures higher than 200℃, showing the potentials for developing as high temperature shape memory alloys. Thermal cycles were performed by DSC instrument on both Cu-Al-Co and Cu-Al-Zr alloys. The results show that Cu-Al-Co loses its martensitic transformation after five thermal cycles, and Cu-Al-Zr exhibits no martensitic transformation in the second thermal cycle.

  7. STUDY ON THE THERMODYNAMICS OF CuO/Al IN SITU Al2O3 REINFORCED Al MATRIX COMPOSITES

    Institute of Scientific and Technical Information of China (English)

    Y.Ma; Y.Hao; S.Z.Kou; X.N.Lu

    2004-01-01

    Based on the experiment and the thermodynamics analysis for CuO/Al system, it is founded that the compounding reaction of CuO/Al under low temperature is available. The general equation of adiabatic temperature of the system is set up, and the influence of resultant concentration and temperature on adiabatic temperature is theoretically analyzed. The values of heat effect of CuO/Al system under different temperature are also caculated.

  8. Entrevista al narrador Tommaso Pincio

    Directory of Open Access Journals (Sweden)

    Franco Zangrilli

    2016-07-01

    Full Text Available El autor Tommaso Pincio, seudónimo di Marco Colapietro (Roma, 1963, es sin lugar a dudas uno de los narradores de mayor proyección en el panorama literario de los últimos tiempos. Iniciada su carrera en 1999 con M., ha publicado títulos como Lo spazio sfinito (2000 y Un amore dell'altro mondo (2002, en el que narra la vida de Kurt Cobain, líder del grupo Nirvana, a través de la visión de un amigo imaginario. A estas dos novelas siguieron La ragazza che non era lei (2005, Gli alieni (2006 o Cinacittà. Memorie del mio delitto efferato (2008, ambientada en una Roma colonizada por los chinos y abandonada por los mismos romanos a causa de problemas medioambientales. Títulos como L'hotel a zero stelle (2011, un ensayo autobiográfico en el que plantea diálogos ficticios con algunos de los grandes nombres de la literatura o la más reciente Panorama (2015, le han hecho valerse un hueco provilegiado entre los nuevos narradores surgidos en estas primeras décadas de siglo. Justamente la publicación de esta última novela, Panorama, ha servido de excusa al profesor Franco Zangrilli, gran conocedor de su obra, a plantearle algunas preguntas sobre temas clave de su producción.

  9. Al-Si/Al2O3 in situ composite prepared by displacement reaction of CuO/Al system

    Directory of Open Access Journals (Sweden)

    Zhang Jing

    2010-02-01

    Full Text Available Al2O3 particle-reinforced ZL109 composite was prepared by in situ reaction between CuO and Al. The microstructure was observed by means of OM, SEM and TEM. The Al2O3 particles in sub-micron sizes distribute uniformly in the matrix, and the Cu displaced from the in situ reaction forms net-like alloy phases with other alloy elements. The hardness and the tensile strength of the composites at room temperature have a slight increase as compared to that of the matrix. However, the tensile strength at 350 ℃ has reached 90.23 MPa, or 16.92 MPa higher than that of the matrix. The mechanism of the reaction in the CuO/Al system was studied by using of differential scanning calorimetry(DSC and thermodynamic calculation. The reaction between CuO and Al involves two steps. First, CuO reacts with Al to form Cu2O and Al2O3 at the melting temperature of the matrix alloy, and second, Cu2O reacts with Al to form Cu and Al2O3 at a higher temperature. At ZL109 casting temperature of 750–780 ℃, the second step can also take place because of the effect of exothermic reaction of the first step.

  10. Derecho civil : aproximaciones al derecho de personas

    OpenAIRE

    2005-01-01

    Las universidades de hoy, siguiendo las últimas tendencias de docencia universitaria que proponen minimizar la participación del profesor en la tarea del aprendizaje, han trasladado al estudiante al esfuerzo de apropiarse de la información necesaria para el conocimiento de las ciencias; Sin embargo, los libros y documentos que se entregan al estudiante para que se empape de los conceptos fundamentales siguen siendo textos fríos y desapacibles cuya utilidad estaba ligada a las explicaciones pr...

  11. Fabrication of AlN-TiC/Al composites by gas injection processing

    Institute of Scientific and Technical Information of China (English)

    YU Huashun; CHEN Hongmei; MA Rendian; MIN Guanghui

    2006-01-01

    The fabrication of AlN-TiC/Al composites by carbon-and nitrogen-containing gas injection into Al-Mg-Ti melts was studied. It was shown that AlN and TiC particles could be formed by the in situ reaction of mixture gas (N2+C2H2+NH3) with Al-Mg-Ti melts. The condition for the formation of AlN was that the treatment temperature must be higher than 1373 K, and the amounts of AlN and TiC increased with the increase of the treatment temperature and the gas injection time.It was considered that AlN was formed by the direct reaction of Al with nitrogen-containing gas at the interface of the gas bubble and the melt. However, the mechanism of TiC formation is a combination mechanism of solution-precipitation and solid-liquid reaction.

  12. Casting fabrication of in situ Al3Ti-Al composites and their wear behaviors

    Institute of Scientific and Technical Information of China (English)

    Chen Tijun; Li Jian; Hao Yuan

    2009-01-01

    The Al3li intermetallic reinforced pure Al, Al-13Si and Al-17Cu matrix composites were prepared by casting method. Their microstructures and dry sliding wear behaviors at room temperature and 100℃ were particularly investigated. The results indicated that the Al3Ti phases in these composites were all in flaky form. But the aspect ratio of the Al3Ti platelets decreased with the increase of Ti content in the pure AI, Al-Cu and Al-Si matrix composites, in order of effectiveness. The effect of Si on the Al3Ti morphology seemed to be greater than that of Cu. The distributions of the Al3Ti platelets were different in the different matrix composites, leading to different grain refining effects. Except for the sub-wear regime of adhesive wear, the plastic deformation induced wear was a dominant wear mechanism for all of the composites at room temperature and 100℃. Increasing the testing temperature, decreasing the Al3Ti content or the hardness of materials could enhance these two wear mechanisms, and thus increase the wear rate. The Al-Cu matrix composite had the best wear resistance, while the pure Al matrix composite showed the worst for the same Ti content. These differences or changes were attributed to the differences in materials' hardness or the strengthening effects of the Al3Ti platelets.

  13. First-principles study of the Al(001)-Al3Nb(001) interfacial properties

    Science.gov (United States)

    Ding, Yanhong; Xu, Rui

    2017-03-01

    The adhesion, interfacial energy and bonding on fcc-Al(001)/D022-Al3Nb(001) interface were investigated using density functional calculations. Considering different terminations of Al3Nb(001) (Al+Nb-terminated and Al-terminated) and stacking sites (top-, bridge- and center-sites), six Al(001)/Al3Nb(001) models were calculated. For the models with same stacking site, Al+Nb-terminated model has larger work of adhesion (Wad) than the Al-terminated one. For the models with same termination, the work of adhesion increases, and the interface energy decreases as the order of center-sited, bridge-sited and top-sited. Al+Nb-terminated-center-sited and Al-terminated-center-sited models are more stable among six models. The interfacial bonding was discussed with analysis of valence electron density distribution and partial density of states (PDOS). The bonding is mainly contributed from Al-Nb covalent bonds and Al-Al metallic interactions.

  14. Electrodeposition of Al from a 1-butylpyrrolidine-AlCl3 ionic liquid

    Directory of Open Access Journals (Sweden)

    Giridhar Pulletikurthi

    2015-12-01

    Full Text Available The addition of 1-butylpyrrolidine to AlCl3 results in the formation of an electrolyte that is suited to Al deposition. The feasibility of electrodepositing Al from the synthesized electrolyte was investigated. Several compositions of AlCl3 and 1-butylpyrrolidine were prepared for this purpose. These mixtures show a different phase behavior at various compositions of AlCl3 and 1-butylpyrrolidine. IR, Raman and NMR spectroscopy were employed to characterize the synthesized liquids. Among the prepared compositions, 1:1.2 mol ratio of 1-butylpyrrolidine:AlCl3 and the upper phase of 1:1.3 mol ratio of 1-butylpyrrolidine:AlCl3 were found to be suitable for Al electrodeposition at room temperature (RT. Uniform and thick (~µm thick layers of Al were obtained on copper at RT. Al deposition occured from the cationic species of AlCl3−xLy+ (where x≤2, y=1–2, and L=1-butylpyrrolidine in this electrolyte. This behavior is contrary to the well investigated classic AlCl3 based ionic liquids, where the deposition of Al occurs mainly from anionic Al2Cl7− ions.

  15. Groente- en fruitafvallen als veevoer : inventarisatie

    NARCIS (Netherlands)

    Vries, de A.

    1982-01-01

    Een onderzoek wat betreft hoeveelheden, bewaring, samenstelling, verteerbaarheid en eventueel aanwezige ongewenste stoffen van de bij- en afvalprodukten van groenten en fruit als veevoer voor herkauwers

  16. Effect of Excess Al in TiO2-Al Powder Mixture and Susceptor Material on Microwave Synthesis of TiAl/Al2O3 Composite

    Directory of Open Access Journals (Sweden)

    P. Radmehr

    2016-03-01

    Full Text Available In this research, TiAl/Al2O3 composite was synthesized from mechanically activated TiO2-Al powder mixtures using microwave heating.The initial powder mixtures were mechanically activated and pressed into cylindrical tablets and then heated in a microwave oven. The effect of different amounts of excess Al and microwave susceptor material (SiC or graphite on the ignition time and the resultant reaction products were evaluated. X-ray diffraction (XRD and scanning electron microscopy (SEM analysis were used for characterization of the synthesized samples. XRD patterns revealed that when there was no excess Al in the initial powder mixture, the main resulting intermetallic phase would be Ti3Al with negligible amounts of TiAl, while with 10 wt% excess Al, TiAl phase could be formed in the composite product.The results also showed that microwave synthesis took place faster and more reproducible when samples were packed in the graphite powder than when placed between two SiC blocks.

  17. Genetics of Familial and Sporadic ALS

    Science.gov (United States)

    2016-03-21

    Amyotrophic Lateral Sclerosis (ALS); Familial Amyotrophic Lateral Sclerosis; Amyotrophic Lateral Sclerosis With Frontotemporal Dementia; Lou Gehrig's Disease; Motor Neuron Disease; Primary Lateral Sclerosis

  18. Interfacial Phenomena in Al/Al, Al/Cu, and Cu/Cu Joints Soldered Using an Al-Zn Alloy with Ag or Cu Additions

    Science.gov (United States)

    Pstruś, Janusz; Gancarz, Tomasz

    2014-05-01

    The studies of soldered joints were carried out in systems: Al/solder/Al, Al/solder/Cu, Cu/solder/Cu, where the solder was (Al-Zn)EUT, (Al-Zn)EUT with 0.5, 1.0, and 1.5 at.% of Ag and (Al-Zn)EUT with 0.5, 1.0, and 1.5 at.% of Cu addition. Brazing was performed at 500 °C for 3 min. The EDS analysis indicated that the composition of the layers starting from the Cu pad was CuZn, Cu5Zn8, and CuZn4, respectively. Wetting tests were performed at 500 °C for 3, 8, 15, and 30 min, respectively. Thickness of the layers and their kinetics of growth were measured based on the SEM micrographs. The formation of interlayers was not observed from the side of Al pads. On the contrary, dissolution of the Al substrate and migration of Al-rich particles into the bulk of the solder were observed.

  19. Atomic structure and oxygen deficiency of the ultrathin aluminium oxide barrier in Al/AlOx/Al Josephson junctions.

    Science.gov (United States)

    Zeng, Lunjie; Tran, Dung Trung; Tai, Cheuk-Wai; Svensson, Gunnar; Olsson, Eva

    2016-07-12

    Al/AlOx/Al Josephson junctions are the building blocks of a wide range of superconducting quantum devices that are key elements for quantum computers, extremely sensitive magnetometers and radiation detectors. The properties of the junctions and the superconducting quantum devices are determined by the atomic structure of the tunnel barrier. The nanoscale dimension and disordered nature of the barrier oxide have been challenges for the direct experimental investigation of the atomic structure of the tunnel barrier. Here we show that the miniaturized dimension of the barrier and the interfacial interaction between crystalline Al and amorphous AlOx give rise to oxygen deficiency at the metal/oxide interfaces. In the interior of the barrier, the oxide resembles the atomic structure of bulk aluminium oxide. Atomic defects such as oxygen vacancies at the interfaces can be the origin of the two-level systems and contribute to decoherence and noise in superconducting quantum circuits.

  20. Atomic structure and oxygen deficiency of the ultrathin aluminium oxide barrier in Al/AlOx/Al Josephson junctions

    Science.gov (United States)

    Zeng, Lunjie; Tran, Dung Trung; Tai, Cheuk-Wai; Svensson, Gunnar; Olsson, Eva

    2016-07-01

    Al/AlOx/Al Josephson junctions are the building blocks of a wide range of superconducting quantum devices that are key elements for quantum computers, extremely sensitive magnetometers and radiation detectors. The properties of the junctions and the superconducting quantum devices are determined by the atomic structure of the tunnel barrier. The nanoscale dimension and disordered nature of the barrier oxide have been challenges for the direct experimental investigation of the atomic structure of the tunnel barrier. Here we show that the miniaturized dimension of the barrier and the interfacial interaction between crystalline Al and amorphous AlOx give rise to oxygen deficiency at the metal/oxide interfaces. In the interior of the barrier, the oxide resembles the atomic structure of bulk aluminium oxide. Atomic defects such as oxygen vacancies at the interfaces can be the origin of the two-level systems and contribute to decoherence and noise in superconducting quantum circuits.

  1. Respuesta inmune adaptativa al rotavirus

    Directory of Open Access Journals (Sweden)

    Manuel A. Franco

    2004-03-01

    Full Text Available

    El rotavirus (RV es un importante patógeno humano y el estudio de la respuesta inmune específica de éste en humanos, es un buen modelo para caracterizar la respuesta inmune de la mucosa intestinal (1. Nuestros estudios han tenido como objetivo la caracterización fenotípica y funcional de las células T y B específicas de RV con énfasis importante en la expresión de marcadores de migración intestinal. Estas células son estudiadas en muestras de sangre periférica de niños y adultos recientemente infectados con RV. Los análisis se hicieron por citometría de flujo y ELISPOT. Estudio de células T: los adultos sanos presentan bajas frecuencias circulantes tanto de LT CD4+, como de LT CD8+ específicos de RV que secretan INF_ .

    Significativamente mayores porcentajes de éstas células se detectaron en adultos sintomáticos infectados por RV. Sin embargo, las frecuencias de LT específicos de RV en todos los grupos de estudio fueron muy bajas comparadas con las observadas para otros patógenos.

    Sorprendentemente, en niños sintomáticos infectados por RV se detectaron bajas frecuencias de LT CD8+ circulantes específicos de RV secretando INF-g y muy pocos o ningún LT CD4+ secretando INF-g o LT CD4+ y CD8+ al secretar IL-13 o IL4 (2,3. Esto sugiere que el RV es un inductor relativamente pobre de células T de memoria secretando estas citoquinas. Los LT circulantes CD4+ , INF-g.+ específicos de RV presentes en adultos

  2. Influence of AlN thickness on AlGaN epilayer grown by MOCVD

    Science.gov (United States)

    Jayasakthi, M.; Juillaguet, S.; Peyre, H.; Konczewicz, L.; Baskar, K.; Contreras, S.

    2016-10-01

    AlGaN/AlN layers were grown by metalorganic chemical vapor deposition (MOCVD) on sapphire substrates. The AlN buffer thickness was varied from 400 nm to 800 nm. The AlGaN layer thickness was 1000 nm. The crystalline quality, thickness and composition of AlGaN were determined using high resolution X-ray diffraction (HRXRD). The threading dislocation density (TDD) was found to decrease with increase of AlN layer thickness. Reciprocal space mapping (RSM) was used to estimate the strain and relaxation between AlGaN and AlN. The optical properties of AlGaN layers were investigated by temperature dependent photoluminescence (PL). PL intensities of AlGaN layers increases with increasing the AlN thickness. The surface morphology of AlGaN was studied by atomic force microscopy (AFM). Root mean square (RMS) roughness values were found to be decreased while increase of AlN thickness.

  3. The cysteine proteinases of the pineapple plant.

    Science.gov (United States)

    Rowan, A D; Buttle, D J; Barrett, A J

    1990-03-15

    The pineapple plant (Ananas comosus) was shown to contain at least four distinct cysteine proteinases, which were purified by a procedure involving active-site-directed affinity chromatography. The major proteinase present in extracts of plant stem was stem bromelain, whilst fruit bromelain was the major proteinase in the fruit. Two additional cysteine proteinases were detected only in the stem: these were ananain and a previously undescribed enzyme that we have called comosain. Stem bromelain, fruit bromelain and ananain were shown to be immunologically distinct. Enzymic characterization revealed differences in both substrate-specificities and inhibition profiles. A study of the cysteine proteinase derived from the related bromeliad Bromelia pinguin (pinguinain) indicated that in many respects it was similar to fruit bromelain, although it was found to be immunologically distinct.

  4. The influence of pH, polyethylene glycol and polyacrylic acid on the stability of stem bromelain

    Directory of Open Access Journals (Sweden)

    Letícia Celia de Lencastre Novaes

    2014-04-01

    Full Text Available Enzyme stability is critical in biotechnology, pharmaceutical and cosmetic industries. Investigations on this subject have drawn attention because of its practical application. Bromelain is a thiol-endopeptidase, obtained from pineapple (Ananas comosus, known for its clinical and therapeutic applications, particularly to selective burn debridement and improvement of antibiotic action and anti-inflammatory activities. To date, the use of bromelain in pharmacological or industrial applications is limited, due to commercial availability, costs, and sensitivity to pH and temperature. Therefore, a better understanding of enzyme stability would be of great interest. The aim of this study was to evaluate bromelain activity and stability in several pH (2.0 to 8.0 and in polyethylene glycol and polyacrylic acid solutions. We observed that bromelain was able to maintain its stability at pH 5.0 for the temperatures studied. PEG solutions increased bromelain stability, but PAA solutions had the opposite effect.

  5. Polymer-based alternative method to extract bromelain from pineapple peel waste.

    Science.gov (United States)

    Novaes, Letícia Celia de Lencastre; Ebinuma, Valéria de Carvalho Santos; Mazzola, Priscila Gava; Pessoa, Adalberto

    2013-01-01

    Bromelain is a mixture of proteolytic enzymes present in all tissues of the pineapple (Ananas comosus Merr.), and it is known for its clinical therapeutic applications, food processing, and as a dietary supplement. The use of pineapple waste for bromelain extraction is interesting from both an environmental and a commercial point of view, because the protease has relevant clinical potential. We aimed to study the optimization of bromelain extraction from pineapple waste, using the aqueous two-phase system formed by polyethylene glycol (PEG) and poly(acrylic acid). In this work, bromelain partitioned preferentially to the top/PEG-rich phase and, in the best condition, achieved a yield of 335.27% with a purification factor of 25.78. The statistical analysis showed that all variables analyzed were significant to the process.

  6. Pineapple fruit bromelain recovery using recyclable functionalized ordered mesoporous silica synthesized from sugarcane leaf ash

    Directory of Open Access Journals (Sweden)

    A. Arumugam

    2013-09-01

    Full Text Available Bromelain, a protease enzyme found in Ananas comosus (Pineapple, was recovered from the fruit juice by adsorption using recyclable functionalized Santa Barbara Acid-15 (SBA-15 synthesized from sugarcane leaf ash. In this work, highly ordered mesoporous silica was synthesized from sugarcane leaf ash by a template-assisted method. It was successfully used as an adsorbent for the recovery of bromelain from pineapple fruit pulp. Amine-functionalized mesoporous silica exhibited a recovery efficiency of 97.89% and a 6.2-fold purification. It was also established that the adsorbent could be easily regenerated by adjusting the pH. In this study, the adsorbent was reused for three cycles without noticeable loss in recovery efficiency. Thus, adsroption using functionalized SBA-15 appears to be a promising alternate separation technique for the recovery of fruit bromelain.

  7. ESTUDIO CINÉTICO ENZIMÁTICO DE LA HIDROLASA A PARTIR DE CÍTRICOS

    Directory of Open Access Journals (Sweden)

    Israel Hernández

    2015-01-01

    Full Text Available En el presente trabajo se evalúa la actividad degradadora de las enzimas obtenidas a partir de las cáscaras de naranja (citrus x sinensis, toronja (citrus paradise y piña (ananas comosus sobre la materia orgánica presente en agua residual. Esta actividad se mide de manera indirecta cuantificando la demanda bioquímica de oxígeno (DQO antes y después del proceso de degradación en función de un periodo de tiempo determinado utilizando el equipo HACH DR/2010, y posteriormente se realizó el estudio cinéticopor el método diferencial e integral con los datos experimentales, obteniendo un orden de reacción de 1 para la pectinasa (naranja, y orden 2 para la bromelina (piña.

  8. Removal of Acid Green 25 from Aqueous Solution by Adsorption

    Directory of Open Access Journals (Sweden)

    R. Parimalam

    2012-01-01

    Full Text Available The adsorptive removal of Acid Green 25 by Ananas Comosus (L Activated carbon was investigated in this study. The effects of initial dye concentration, contact time, pH and temperature were studied for the adsorption of Acid Green 25 in batch mode. At 100 mg/L of initial dye concentration the adsorbent removes 182.6 mg/g of dye from solution; it further increases on increasing the temperature. The calculated values of ∆G° indicate that the adsorption process is spontaneous, negative ∆H° indicate that the adsorption process is exothermic and the positive value of ∆S° indicates the increase in randomness. The rate of dye adsorption follows pseudo second order model with an r2 value of 0. 999. Standard adsorption isotherms were used to fit the experimental equilibrium data. The Langmuir, Freundlich, and Tempkin models are appropriate to explain the adsorption phenomenon with good fit.

  9. TEORES DE POLIFENÓIS DE CAULE E FOLHA DE QUATRO CULTIVARES DE ABACAXIZEIRO

    Directory of Open Access Journals (Sweden)

    SANTOS MÔNICA ALESSANDRA TEIXEIRA DOS

    2001-01-01

    Full Text Available Teores de polifenóis foram determinados nos resíduos agrícolas - caule e folha -do abacaxizeiro (Ananas comosus (L. Merrill das cultivares Pérola, Perolera, Smooth Cayenne e Primavera, cultivadas em Ijací-MG, com o objetivo de subsidiar a possível utilização destes resíduos para consumo humano e/ou animal. De acordo com os resultados obtidos, nenhuma das cultivares estudadas apresentou teores de polifenóis acima de 1%, nível considerado alto e prejudicial à digestibilidade de proteínas. A folha apresentou teores de polifenóis superiores aos do caule em todas as cultivares analisadas. Não houve diferenças significativas entre as cultivares, mas a Smooth Cayenne e a Perolera apresentaram os maiores e menores teores de polifenóis, respectivamente.

  10. Effect of pH, temperature and alcohols on the stability of glycosylated and deglycosylated stem bromelain

    Indian Academy of Sciences (India)

    Rizwan Hasan Khan; Sheeba Rasheedi; Soghra Khatun Haq

    2003-12-01

    The biological significance of the carbohydrate moiety of a glycoprotein has been a matter of much speculation. In the present work, we have chosen stem bromelain from Ananas comosus as a model to investigate the role of glycosylation of proteins. Stem bromelain is a thiol protease which contains a single hetero-oligosaccharide unit per molecule. Here, the deglycosylated form of the enzyme was obtained by periodate oxidation. The differences in the glycosylated and deglycosylated forms of the glycoprotein have been studied at various temperatures and pH values, using probes such as loss of enzyme activity and by the changes in fluorescence and circular dichroism spectra. Deglycosylated bromelain showed decreased enzyme activity and perturbed fluorescence and circular dichroism spectra. In addition to this, a comparative study of their activities in different organic solvents showed a marked decrease in case of deglycosylated form of the enzyme. It is thus concluded that glycosylation contributes towards the functional stability of glycoenzymes.

  11. Concentrations of plant regulators in the blanching in vitro of pineapple of the fieldConcentrações de reguladores vegetais no estiolamento in vitro de ananás do campo

    Directory of Open Access Journals (Sweden)

    Antônio Clarete de Oliveira

    2011-07-01

    Full Text Available The ornamental pineaple Ananas comosus (L. Merr. var. ananassoides (Baker Coppens & F. Leal, also denominated of pineapple of the field or it crowns green it is a plant still little explored economically in spite of the ornamental potential as " cut flower ". Studies about the propagation of the pineapple of the field are scarce, in this sense, the micropropagation of this can represent a progress for the commercial production of the species. The objective of the work was to evaluate the different combinations of 6-BAP and GA3 in the multiplication and development of the explants of Ananas comosus (L. Merr. var. ananassoides (Baker Coppens & F. Leal. The randomized experimental desing was used, with a factorial scheme 4x4 consisting of four concentrations of 6-BAP (0; 0,25; 0,5 and 1,0 mg.L-1 and four concentrations of GA3 (0; 0,5; 1,0 and 2,0 mg.L-1 combined. According to the results, the interaction 1,0 mg.L-1 of 6-BAP and 0,5 mg.L-1 of GA3 provided larger sprouts number. The addition of 6-BAP propitiated a reduction of the bud length and of the roots number of explants. The addition of GA3 caused a larger length of the aerial part and it didn't influence in the rooting of the explants.O abacaxizeiro ornamental Ananas comosus (L. Merr. var. ananassoides (Baker Coppens & F. Leal, também denominado de ananás do campo ou coroa verde, é uma planta ainda pouco explorada economicamente, apesar do potencial ornamental como “flor de corte”. Estudos sobre a propagação do ananás do campo são escassos, neste sentido, a micropropagação desta, pode representar um avanço para a produção comercial da espécie. O objetivo do trabalho foi avaliar as diferentes combinações de 6-BAP e GA3 na multiplicação e desenvolvimento dos explantes de Ananas comosus (L. Merr. var. ananassoides (Baker Coppens & F. Leal. Para a condução do experimento foram utilizados brotos de abacaxizeiro ornamental pré-estabelecidos in vitro. O delineamento utilizado

  12. Kus on al-Zarqawi? / Aadu Hiietamm

    Index Scriptorium Estoniae

    Hiietamm, Aadu, 1954-

    2005-01-01

    Suurbritannia nädalalehe Sunday Times andmetel on Iraagi tagaotsituim mässaja, terrorivõrgustiku al Qaeda liitlane Abu-Musab al-Zarqawi pärast USA raketirünnakus haavatasaamist riigist põgenenud

  13. Al-Farabi dan Filsafat Kenabian

    Directory of Open Access Journals (Sweden)

    Qosim Nursheha Dzulhadi

    2014-03-01

    Full Text Available Artikel ini mengulas pemikiran al-Farabi tentang konsep kenabian dalam Islam. Masalah berawal dari adanya pemikiran yang menolak konsep kenabian pada umumnya dan kenabian Nabi Muhammad SAW khususnya. Sebagaimana yang dilontarkan oleh Muhammad Ahmad ibn al-Ruwandi. Ia mengatakan bahwa nabi sebenarnya tidak diperlukan manusia karena Tuhan telah mengaruniakan akal kepada manusia tanpa terkecuali. Dengan akal ini, manusia dapat mengetahui Tuhan beserta segala nikmat Nya dan dapat pula mengetahui perbuatan baik dan buruk, menerima suruhan dan larangan-Nya. Dengan demikian, nabi dengan segala fungsinya tidak diperlukan lagi. Bahkan, kitab suci pun tidak berguna untuk dibaca. Lebih berguna membaca buku filsafat Epicurus, Plato, Aristoteles, dan buku astronomi, logika, serta obat-obatan. Hal ini tentunya bertolak belakang dengan yang diajarkan dalam Islam. Beriman kepada nabi nabi merupakan hal inti dalam ajaran agama ini. Atas dasar itu al-Farabi bereaksi keras. Baginya, pandangan Ibn al-Ruwandi di atas tidak dapat dibenarkan, khususnya dari sisi akidah Islam. Sebagai al-Mu‘allim al-Tsani (Guru Kedua, karena prestasinya dalam menjelaskan dan mengulas-ulang filsafat Aristoteles, al-Farabi mengkritik secara sistematik pandangan menyimpang al-Ruwandi di atas.

  14. Diana Al-Hadid: Identity and Heritage

    Science.gov (United States)

    Jungerberg, Tom; Smith, Anna; Borsh, Colleen

    2012-01-01

    Diana Al-Hadid's sculptures reflect the many locations, cultures, histories, and mythologies that have shaped her as an artist. In large-scale works which have the appearance of architectural ruins, Al-Hadid employs imagery drawn from many diverse interests including science and technology, history, and literature. She also incorporates images and…

  15. The role of inclusions in ALS pathogenesis

    NARCIS (Netherlands)

    V. van Dis (Vera)

    2012-01-01

    textabstractAmyotrophic lateral sclerosis (ALS) is a devastating neurodegenerative disease that primarily afflicts motor neurons, leading to paralysis and death within 3 to 5 years after diagnosis. Genetic studies have uncovered multiple genetic defects causing familial forms of ALS and mutations oc

  16. Ceramic foams for Al-recycling

    Energy Technology Data Exchange (ETDEWEB)

    Luyten, J.; Vandermeulen, W.; Schutter, F. de [VITO, Materials Technology, Mol (Belgium); Simensen, C. [Sintef, Materials Technology, Blindern, Oslo (Norway); Ryckeboer, M. [Remi Claeys Aluminium, Lichtervelde (Belgium)

    2006-08-15

    The recycling of Al scrap requires an efficient purification step. In this investigation a filter technique is used to remove intermetallic compounds containing Fe, Mn and Si out of the molten Al. The paper describes the synthesis of the filters, the construction of the filter installation, and the results of the different filter tests. (Abstract Copyright [2006], Wiley Periodicals, Inc.)

  17. Konsepsi Ibnu Jarir Al-Tabari Tentang Al-Qur’an, Tafsir Dan Ta’wil

    Directory of Open Access Journals (Sweden)

    A.M Ismatulloh

    2012-12-01

    Full Text Available The objective of this research is to describe the concept of Ibnu Jarir al-Tabari about al-Qur’an, tafsir and ta’wil. Ibn Jarir al-Tabari is an outstanding expert in the classic Islamic tradition. His knowledge in hadits, fiqh, lughah, history, and tafseer is unquestionable. This study uses an analytical descriptive design. The findings show that: Firstly, in defining al-Qur’an, al-Tabari focused on the al-Qur’an as an Arabic language text, not in other language. Secondly, al-Qur’an is written in sab’ah ahruf. It means that there are 7 different kinds of language with the same meaning. Thirdly, al-Qur’an comes from seven heaven’s door. Fourthly, there is no different word between tafseer and ta’wil. They have same meaning. These concepts are different to other concepts proposed by other ulama.

  18. Kinetic Monte Carlo of transport processes in Al/AlOx/Au-layers: Impact of defects

    Directory of Open Access Journals (Sweden)

    Benedikt Weiler

    2016-09-01

    Full Text Available Ultrathin films of alumina were investigated by a compact kMC-model. Experimental jV-curves from Al/AlOx/Au-junctions with plasma- and thermal-grown AlOx were fitted by simulated ones. We found dominant defects at 2.3-2.5 eV below CBM for AlOx with an effective mass mox∗=0.35 m0 and a barrier EB,Al/AlOx≈2.8 eV in agreement with literature. The parameterization is extended to varying defect levels, defect densities, injection barriers, effective masses and the thickness of AlOx. Thus, dominant charge transport processes and implications on the relevance of defects are derived and AlOx parameters are specified which are detrimental for the operation of devices.

  19. Data supporting Al-Abed et al., Environ. Sci.: Nano, 2016,

    Data.gov (United States)

    U.S. Environmental Protection Agency — Data files representing each of the Figures and Tables published in Al-Abed et al., Environ. Sci.: Nano, 2016, 3, 593. The data file names identify the Figure or...

  20. Effect of Al coating conditions on laser weldability of Al coated steel sheet

    Institute of Scientific and Technical Information of China (English)

    Jung-Han LEE; Jong-Do KIM; Jin-Seok OH; Seo-Jeong PARK

    2009-01-01

    Al coated steel sheets with excellent heat resistance, thermal reflection, and corrosion resistance are widely used in various applications. The laser weldability of the Al coated steel sheet for full penetration welding was reported. The phenomenon caused by intermixed aluminum and behavior of aluminum in the weld were investigated. Al coated steel sheets that have various thickness and coating mass were prepared for laser welding. The effects of parameters such as welding conditions and Al coating conditions were investigated. Al content mixed in the weld after laser welding was evaluated, and then a correlation between the mixed Al and mechanical properties was investigated. The results show that the Al-rich zones which have Fe-Al intermetallic compounds are found in the weld. The intermetallic compounds cause the decreased strength of the weld.

  1. Wallada Bint al Mustakfi entre dos culturas

    OpenAIRE

    shreen Mahmoud Al Refai

    2013-01-01

    La princesa cordobesa Wallada Bint al Mustakfi bil-lah, hija del califa al Mustakfi Muhammad Ibn Abdel Rahman Ibn Obaidil-lah Ibn Al Nasir lidin Al-lah era una poetisa estimable, a quien los grandes poetas no podían superar. Era una mujer única, guapa y decente. Mantenía una relación sentimental con el  poeta y visir Ibn Zaydún. Dicha pasión, narrada por los poemas escritos por ambos amantes, se terminó por la separación debida a los celos, también de ambos;  al poeta le gustó la voz de una e...

  2. Thermoluminescence properties of AlN ceramics

    DEFF Research Database (Denmark)

    Trinkler, L.; Christensen, P.; Agersnap Larsen, N.

    1998-01-01

    The paper describes thermoluminescence (TL) properties of AlN:Y2O3 ceramics irradiated with ionising radiation. A high TL sensitivity of AlN:Y2O3 ceramics to radiation encouraged a study of the AlN ceramics for application as a dosimetric material. The paper presents experimental data on: glow...... curve, emission spectrum, dose response, energy dependence, influence of heating rate and fading rate. The measured TL characteristics were compared with those of well-known, widely used TLDs, i.e. LiF:Mg,Ti, LiF:Mg,Cu,P and Al2O3:C. It is concluded that AlN:Y2O3 ceramics showing a radiation sensitivity...

  3. The neuropathology of FTD associated With ALS.

    Science.gov (United States)

    Mackenzie, Ian R A

    2007-01-01

    There is increasing recognition of a clinical overlap between frontotemporal dementia (FTD) and amyotrophic lateral sclerosis (ALS). Recent advances in our understanding of the neuropathologic, biochemical, and genetic basis of these conditions provides evidence for a common underlying pathogenesis. The neuropathology in most cases of FTD with ALS is a subtype of frontotemporal lobar degeneration, characterized by neuronal inclusions that are immunoreactive for ubiquitin but not tau (frontotemporal lobar degeneration with ubiquitinated inclusions). These cases show significant pathologic overlap with clinically pure FTD and those with classic ALS. Moreover, the ubiquitinated pathologic protein in all these conditions has recently been identified as TDP-43. A number of families have been reported with autosomal dominant FTD-ALS linked to chromosome 9p and these also have TDP-43-positive frontotemporal lobar degeneration with ubiquitinated inclusions pathology. Together, these findings suggest that FTD-ALS is part of a clinicopathologic spectrum of disease, now identified as TDP-43 proteinopathies.

  4. NiAl alloys for structural uses

    Science.gov (United States)

    Koss, D. A.

    1991-01-01

    Alloys based on the intermetallic compound NiAl are of technological interest as high temperature structural alloys. These alloys possess a relatively low density, high melting temperature, good thermal conductivity, and (usually) good oxidation resistance. However, NiAl and NiAl-base alloys suffer from poor fracture resistance at low temperatures as well as inadequate creep strength at elevated temperatures. This research program explored macroalloying additions to NiAl-base alloys in order to identify possible alloying and processing routes which promote both low temperature fracture toughness and high temperature strength. Initial results from the study examined the additions of Fe, Co, and Hf on the microstructure, deformation, and fracture resistance of NiAl-based alloys. Of significance were the observations that the presence of the gamma-prime phase, based on Ni3Al, could enhance the fracture resistance if the gamma-prime were present as a continuous grain boundary film or 'necklace'; and the Ni-35Al-20Fe alloy was ductile in ribbon form despite a microstructure consisting solely of the B2 beta phase based on NiAl. The ductility inherent in the Ni-35Al-20Fe alloy was explored further in subsequent studies. Those results confirm the presence of ductility in the Ni-35Al-20Fe alloy after rapid cooling from 750 - 1000 C. However exposure at 550 C caused embrittlement; this was associated with an age-hardening reaction caused by the formation of Fe-rich precipitates. In contrast, to the Ni-35Al-20Fe alloy, exploratory research indicated that compositions in the range of Ni-35Al-12Fe retain the ordered B2 structure of NiAl, are ductile, and do not age-harden or embrittle after thermal exposure. Thus, our recent efforts have focused on the behavior of the Ni-35Al-12Fe alloy. A second parallel effort initiated in this program was to use an alternate processing technique, mechanical alloying, to improve the properties of NiAl-alloys. Mechanical alloying in the

  5. Stabilization of itinerant (band) magnetism in FeAl by Ga substitution for Al

    Energy Technology Data Exchange (ETDEWEB)

    Papaconstantopoulos, D. A.; Mazin, I. I.; Hathaway, K. B.

    2001-06-01

    Band structure calculations of FeGa{sub 1{minus}x}Al{sub x} have been performed, to further investigate the stability of ferromagnetism in FeAl. The Stoner parameter increases by about 20% at the FeGa end. This is also confirmed by our spin-polarized calculations. We conclude that Ga substitution for Al is likely to stabilize the elusive (or illusive) ferromagnetic state in FeAl. {copyright} 2001 American Institute of Physics.

  6. XAFS Study of the Al K-Edge in NaAlH4

    NARCIS (Netherlands)

    Balde, C.P.; Mijovilovich, A.E.; Koningsberger, D.C.; van der Eerden, A.M.J.; Smith, A.D.; de Jong, K.P.; Bitter, J.H.

    2007-01-01

    Al K-edge (1561.1 eV) XANES and EXAFS spectra of unoxidized NaAlH4, a hydrogen storage material, have been recorded in He atmosphere in a special in situ low-energy cell. The XANES spectrum of NaAlH4 is reported and compared to that of oxidized NaAlH4, thus illustrating the power of XANES spectrosco

  7. Memes als moderne Bildungsmedien: Humor als Medium pädagogischer Interaktion zur Wissenskonstruktion im Physikunterricht

    OpenAIRE

    Prescher, Thomas; Thees, Michael

    2015-01-01

    Memes stellen ein modernes Bildmedium dar. Im Beitrag wird die Frage verfolgt, ob Memes mit ihrem inhärenten Humor innerhalb des Unterrichts auch als Bildungsmedium dienen und didaktisch genutzt werden können. Dazu wird Humor als generalisiertes Kommunikationsmedium pädagogischer Interaktion beschrieben, um Memes als moderne Bildmedien - in ihrer Wirkung als humorvolle Form der affektiven und kognitiven Inkongruenzstiftung - zur Entwicklung von Fachkonzepten im Physikunterricht fassen zu könn...

  8. Corrosion Studies of 2195 Al-Li Alloy and 2219 Al Alloy with Differing Surface Treatments

    Science.gov (United States)

    Danford, M. D.; Mendrek, M. J.

    1998-01-01

    Corrosion studies of 2195 Al-Li and 2219 Al alloys have been conducted using the scanning reference electrode technique (SRET) and the polarization resistance (PR) technique. The SRET was used to study corrosion mechanisms, while corrosion rate measurements were studied with the PR technique. Plates of Al203 blasted, soda blasted and conversion coated 2219 Al were coated with Deft primer and the corrosion rates studied with the EIS technique. Results from all of these studies are presented.

  9. High temperature creep behaviour of Al-rich Ti-Al alloys

    Science.gov (United States)

    Sturm, D.; Heilmaier, M.; Saage, H.; Aguilar, J.; Schmitz, G. J.; Drevermann, A.; Palm, M.; Stein, F.; Engberding, N.; Kelm, K.; Irsen, S.

    2010-07-01

    Compared to Ti-rich γ-TiAl-based alloys Al-rich Ti-Al alloys offer an additional reduction of in density and a better oxidation resistance which are both due to the increased Al content. Polycrystalline material was manufactured by centrifugal casting. Microstructural characterization was carried out employing light-optical, scanning and transmission electron microscopy and XRD analyses. The high temperature creep of two binary alloys, namely Al60Ti40 and Al62Ti38 was comparatively assessed with compression tests at constant true stress in a temperature range between 1173 and 1323 K in air. The alloys were tested in the cast condition (containing various amounts of the metastable phases Al5Ti3 and h-Al2Ti) and after annealing at 1223 K for 200 h which produced (thermodynamically stable) lamellar γ-TiAl + r-Al2Ti microstructures. In general, already the as-cast alloys exhibit a reasonable creep resistance at 1173 K. Compared with Al60Ti40, both, the as-cast and the annealed Al62Ti38 alloy exhibit better creep resistance up to 1323 K which can be rationalized by the reduced lamella spacing. The assessment of creep tests conducted at identical stress levels and varying temperatures yielded apparent activation energies for creep of Q = 430 kJ/mol for the annealed Al60Ti40 alloy and of Q = 383 kJ/mol for the annealed Al62Ti38 material. The latter coincides well with that of Al diffusion in γ-TiAl, whereas the former can be rationalized by the instability of the microstructure containing metastable phases.

  10. Dakwah Kultural Bayt al-Qur’an al-Akbar Ukiran Kayu Khas Melayu Palembang

    Directory of Open Access Journals (Sweden)

    Reza Pahlevi

    2016-07-01

    Full Text Available Penelitian ini berusaha mengungkap berbagai fenomena yang menggambarkan serangkaian kegiatan dakwah kultural Bayt al-Qur’an al-Akbar Ukiran Khas Melayu Palembang. Sehingga dapat diperoleh sejumlah informasi mengenai berbagai upaya penerapan program yang dimaksud. Penelitian ini dapat disimpulkan, pertama kontribusi Bayt al-Qur’an al-Akbar Ukiran Khas Melayu Palembang terhadap perkembangan metode dakwah kultural adalah dakwah melalui seni. Kedua, Bayt al-Qur’an al-Akbar Ukiran Khas Melayu Palembang sebagai tempat Wisata Religi yang sangat signifikan.   This research tries to reveal the various phenomenon that describe a series of cultural propaganda activities Bayt al-Qur’an al-Akbar Ukiran Khas Melayu Palembang. In order to obtain some informations about the efforts of the implementation of the program in question. It can be concluded, the first contribution of Bayt al-Qur’an al-Akbar Ukiran Khas Melayu Palembang on the development of methods of cultural propaganda is propaganda through art. Second Bayt al-Qur’an al-Akbar Ukiran Khas Melayu Palembang as a very significant Religious Tourism.

  11. Das Américas para o Mundo: origem, domesticação e dispersão do abacaxizeiro From the Americas to the World: origin, domestication and dispersion of pineapple

    Directory of Open Access Journals (Sweden)

    Maraisa Crestani

    2010-06-01

    Full Text Available O abacaxi (Ananas comosus (L. Merril, fruto símbolo de regiões tropicais e subtropicais, originário das Américas, foi difundido para todo o mundo, principalmente pelos navegantes europeus, em razão de seu aroma e sabor característicos e exuberante aparência. Batizado como o "Rei das Frutas Coloniais", o abacaxi encontra-se entre as 11 frutas mais produzidas no mundo, sendo cultivada e consumida pelos cinco continentes, e o Brasil destaca-se como maior produtor. Assim, o objetivo desta revisão bibliográfica é abordar a história da cultura do abacaxi, contemplando sua botânica, sua origem e dispersão pelo mundo, seu cenário atual, também aspectos relacionados à conservação de germoplasma e ao melhoramento genético.The pineapple(Ananas comosus (L. Merril, fruit-symbol of tropical and subtropical regions, originated in the Americas, was widespread throughout the world mainly by european navigators because of its aroma and flavor, and lush appearance. Named as "King of Colonial Fruits", pineapple is one of the eleven most produced fruit in the world, growing consumed in every continent, with Brazil as the largest producer. Then, the objective of this literature review is to approach the pineapple history, contemplating its botany, origin and dispersion in the world and its current scenario and issues related to germplasm conservation and breeding.

  12. Japanese version of the ALS-FTD-Questionnaire (ALS-FTD-Q-J).

    Science.gov (United States)

    Watanabe, Yasuhiro; Beeldman, Emma; Raaphorst, Joost; Izumi, Yuishin; Yoshino, Hiide; Masuda, Michihito; Atsuta, Naoki; Ito, Satoru; Adachi, Tadashi; Adachi, Yoshiki; Yokota, Osamu; Oda, Masaya; Hanashima, Ritsuko; Ogino, Mieko; Ichikawa, Hiroo; Hasegawa, Kazuko; Kimura, Hideki; Shimizu, Toshio; Aiba, Ikuko; Yabe, Hayato; Kanba, Makoto; Kusumi, Kimiyoshi; Aoki, Tetsuya; Hiroe, Yu; Watanabe, Hirohisa; Nishiyama, Kazutoshi; Nomoto, Masahiro; Sobue, Gen; Nakashima, Kenji

    2016-08-15

    Amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD) share common clinical, genetic and neuropathological features. Some ALS patients have behavioral/personality changes, which could result in significant obstacles in the care provided by family members and caregivers. An easy screening tool would contribute greatly to the evaluation of these symptoms. We translated the ALS-FTD-Questionnaire, developed in the Netherlands, into Japanese (ALS-FTD-Q-J) and examined the clinimetric properties (internal consistency, construct and clinical validity). Patients with ALS and/or behavioral variant FTD (bvFTD) were evaluated alongside healthy controls in this multicenter study. All ALS patients, regardless of bvFTD status, were further evaluated by the frontal behavioral inventory (FBI) and for frontal/executive function, cognition, anxiety/depression, and motor functions. Data from 146 subjects were analyzed: ALS (92), ALS-bvFTD (6), bvFTD (16), and healthy controls (32). The internal consistency of the ALS-FTD-Q-J was good (Cronbach α=0.92). The ALS-FTD-Q-J showed construct validity as it exhibited a high correlation with the FBI (r=0.79). However, correlations were moderate with anxiety/depression and low with cognitive scales, in contrast to the original report, i.e. a moderate correlation with cognition and a low correlation with anxiety/depression. The ALS-FTD-Q-J discriminated ALS patients from (ALS-)bvFTD patients and controls. Thus, the ALS-FTD-Q-J is useful for evaluating Japanese ALS/FTD patients.

  13. WAHDAT AL WUJUD DALAM PEMIKIRAN IBNU ARABI

    Directory of Open Access Journals (Sweden)

    Abd Halim Rofi'ie

    2013-09-01

    Full Text Available Ibnu Arabi, the Grand Master of Islam, is a great thinker whose controversial thought, wahdat al-wujud, has significantly influenced the mystiques of Islam. The meaning of wahdat al-wujud is basically two folds, i.e. that Being is the only One which exists and that the universe is merely a manifestation of the One. The One Being here refers to Allah SWT. The One covers up all the existing phenomena and functions as a resource which emanates the entire universe. In this context, He is referred to as al-Haqiqat al-Muhammadiyah. He might also be viewed as both the Universal Soul for His being the source of cosmos that governs the universe and the Universal Body for His reflecting any actions on every single micro existence that forms the universe. Furthermore, when His existence is deemed as a jauhar (jewel that face-up any occurrences, He is typically known as al-haba’. The concept of wahdat al-wujud can actually be simplified into the principle of laa maujuda illa wujud al-wahid (no existence except the Existing One, the Being. The One might reveal His own existence into different martabat or levels: Martabat Ahadiyah, Martabat Wahidiyah, and Martabat Tajalli Syuhudi. Those standing points trigger Ibnu Arabi to believe that any kinds of realities or existences are nothing but a true reflection of the One.

  14. Thermal diffusion in Ni/Al multilayer

    Energy Technology Data Exchange (ETDEWEB)

    Swain, M.; Bhattacharya, D.; Singh, S.; Basu, S. [Solid State Physics Division, Bhabha Atomic Research Center, Mumbai 400085 (India); Gupta, M. [UGC-DAE-Consortium for Scientific Research, University Campus, Khandwa Road, Indore 452017 (India)

    2013-02-05

    Two Ni/Al multilayers deposited by ion beam sputtering of nominal design [Ni(200A)/Al(100A)] Multiplication-Sign 5 and [Ni(50A)/Al(227A)] Multiplication-Sign 5 on Si substrates were annealed at 200 Degree-Sign C. As-deposited and annealed samples were characterized by x-ray diffraction (XRD) and x-ray reflectometry (XRR). The effort was to study the path of alloying in the above two multilayers of same elements but of opposite stoichiometric ratio. We find distinct differences in alloying of these samples.

  15. Juan Goytisolo en al aule de ELE

    OpenAIRE

    Zubia Fernandez, Daniel

    2004-01-01

    Al integrar en el aula de ELE artículos periodísticos sobre un determinado escritor como una práctica de expresión oral y escrita, se aproxima al alumno principiante de ELE al universo literario del autor escogido con el propósito de entrelazar la enseñanza de la lengua y la de la(s) literatura(s) en lengua española. Con este enfoque se estimula la capacidad lectora del alumno en la lengua meta ya que los textos periodísticos constituyen modelos ejemplares para este propósito d...

  16. Characterization of different-Al-content AlGaN/GaN heterostructures on sapphire

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    Al x Ga 1-x N/GaN high-electron-mobility transistor (HEMT) structures with Al composition ranging from x = 0.13 to 0.36 are grown on sapphire substrates by low-pressure metalorganic chemical vapor deposition (LP-MOCVD). The effects of Al content on crystal quality, surface morphology, optical and electrical characteristics of the AlGaN/GaN heterostructures have been analyzed. Although high Al-content (36%) heterostructure exhibits a distinguished photoluminescence peak related to recombination between the two-dimensional electron gas and photoexcited holes (2DEG-h), its crystal quality and rough surface morphology are poor. 2DEG mobility increases with the Al content up to 26% and then it apparently decreases for high Al-content (36%) AlGaN/GaN heterostructure. The increase of sheet carrier density with the increase of Al content has been observed. A high mobility at room temperature of 2105 cm 2 /V s with a sheet carrier density of n s = 1.10 × 10 13 cm -2 , for a 26% Al-content AlGaN/GaN heterostructure has been obtained, which is approaching state-of-the-art for HEMT grown on SiC. Sheet resistance as low as 274 Ω/□ has also been achieved.

  17. Tracing Al Shabaab’s Decision to Cooperate with Al Qaeda in Somalia (2008

    Directory of Open Access Journals (Sweden)

    Adlini Ilma Ghaisany Sjah

    2014-02-01

    Full Text Available 'Al Shabaab, a Somali insurgent with predominantly nationalistic causes (alongside transnational [global Jihad] and Islamic goals made the choice to fight on behalf of Al Qaeda in 2008. The decision to do so contrasted with Al Shabaab’s previous behaviour of actively denying cooperation and distancing itself from Al Qaeda. This study aims to uncover factors that contributed to Al Shabaab’s decision through the use of process-tracing. The results show that Al Shabaab’s declaration of cooperation with Al Qaeda in August 2008 was brought about by a series of events that traced back to four factors: public reactions to the implementation of a Salafi ideology, the shift to a radical leadership, Al Shabaab’s inability to maintain stability in areas under its control and its increasing attacks by Al Shabaab on the Somali public. Al Shabaab pursued cooperation with Al Qaeda after realizing that their nationalistic preference was no longer achievable in the near future.' ' '

  18. InAlN/AlN/GaN heterostructures for high electron mobility transistors

    Science.gov (United States)

    Usov, S. O.; Sakharov, A. V.; Tsatsulnikov, A. F.; Lundin, V. W.; Zavarin, E. E.; Nikolaev, A. E.; Yagovkina, M. A.; Zemlyakov, V. E.; Egorkin, V. I.; Ustinov, V. M.

    2016-08-01

    The results of development of InAlN/AlN/GaN heterostructures, grown on sapphire substrates by metal-organic chemical vapour deposition, and high electron mobility transistors (HEMTs) based on them are presented. The dependencies of the InAlN/AlN/GaN heterostructure properties on epitaxial growth conditions were investigated. The optimal indium content and InAlN barrier layer thicknesses of the heterostructures for HEMT s were determined. The possibility to improve the characteristics of HEMTs by in-situ passivation by Si3N4 thin protective layer deposited in the same epitaxial process was demonstrated. The InAlN/AlN/GaN heterostructure grown on sapphire substrate with diameter of 100 mm were obtained with sufficiently uniform distribution of sheet resistance. The HEMTs with saturation current of 1600 mA/mm and transconductance of 230 mS/mm are demonstrated.

  19. Preparation and Mechanical Properties of Al2O3/Al Laminated Ceramic Matrix Composites

    Institute of Scientific and Technical Information of China (English)

    HUANG Kangming; LI Weixin; XIE Binhuan; RAO Pinggen; PENG Cheng; CHEN Dabo; WU Jianqing

    2011-01-01

    Three series of Al2O3/Al laminated ceramic matrix composites,named SPA,SPV and HP,were fabricated by different methods.SPA and SPV were prepared using Al2O3 slices and Al slurry via screen printing and subsequent heat treatment in air or vacuum.HP samples were made by hot pressing the layered stack of Al foils and Al2O3 slices.SEM and XRD were applied to analyze the microstructure and the interlayer crystal phase.The bending strength,fracture toughness and fracture work of the samples made by the three methods were measured and compared.The results show that the composites have much better toughness and higher fracture work than the Al2O3 slice.Among the samples made by the three methods,the samples made by hot pressing have the optimum mechanical performance.The displacement-load curves and fracture mechanism were analyzed.

  20. The Y-Ag-Al system

    Energy Technology Data Exchange (ETDEWEB)

    Gumenyuk, T.M.; Kuz' ma, Yu.B.; Stel' makhovych, B.M. [L' vov State Univ. (Ukraine). Dept. of Anal. Chem.

    2000-03-14

    The interaction between the components in the Y-Ag-Al system at 870 K has been studied using X-ray analysis. The phase diagram in the region up to 50 at.% of yttrium has been constructed. The limits of the solid solution regions of the binary compounds and the homogeneity ranges of the ternary ones have been determined. The crystal structures of the ternary aluminides Y{sub 3}Ag{sub 2}Al{sub 7} (Ca{sub 3}Cu{sub 2}Al--type structure, space group R anti 3m, a=0.55422(2), c=2.6259(1) nm) and YAg{sub 0.6}Al{sub 1.4} (KHg{sub 2}-type structure, space group Imma, a=0.45399(2), b=0.71551(3), c=0.78712(4) nm) have been studied for the first time. (orig.)

  1. Calcium platinum aluminium, CaPtAl

    Directory of Open Access Journals (Sweden)

    Charles Fon Abi

    2011-10-01

    Full Text Available A preliminary X-ray study of CaPtAl has been reported previously by Hulliger [J. Alloys Compd (1993, 196, 225–228] based on X-ray powder diffraction data without structure refinement. With the present single-crystal X-ray study, we confirm the assignment of the TiNiSi type for CaPtAl, in a fully ordered inverse structure. All three atoms of the asymmetric unit have .m. site symmetry. The structure features a ∞3[AlPt] open framework with a fourfold coordination of Pt by Al atoms and vice versa. The Ca atoms are located in the large channels of the structure.

  2. 78 FR 4967 - Alabama Disaster #AL-00046

    Science.gov (United States)

    2013-01-23

    ... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS ADMINISTRATION Alabama Disaster AL-00046 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY... adversely affected by the disaster: Primary Counties: Mobile; Pike. Contiguous Counties: Alabama:...

  3. Smoking Tied to Shorter Survival with ALS

    Science.gov (United States)

    ... page: https://medlineplus.gov/news/fullstory_161094.html Smoking Tied to Shorter Survival With ALS Tobacco use ... 22, 2016 WEDNESDAY, Sept. 21, 2016 (HealthDay News) -- Smoking may speed progression of Lou Gehrig's disease and ...

  4. Thermal Conductivity of Al-Salt Composites

    Science.gov (United States)

    Li, Peng; Zhang, Mei; Wang, Lijun; Seetharaman, Seshadri

    2015-11-01

    With a view to examine the possibility of estimating the content of entrapped metallic aluminium in the salt cake from aluminium remelting, the thermal diffusivity of reference composites of KCl-NaCl-Al was measured as a function of aluminium metal content at room temperature. The thermal conductivity of the reference composites was found to increase with the metallic Al content. The lumped parameter model approach was carried out to discuss the influence of different geometry arrangements of each phase, viz. air, salts and metallic aluminium on the thermal conductivity. Application of the present results to industrial samples indicates that factors such as the interfacial condition of metallic Al particles have to be considered in order to estimate the amount of entrapped Al in the salt cake.

  5. Are RENiAl hydrides metallic?

    Energy Technology Data Exchange (ETDEWEB)

    Eichinger, Karl; Havela, Ladislav; Prokleska, Jan; Stelmakhovych, Olha; Danis, Stanislav [Faculty of Mathematics and Physics, Charles Univ., Prague (Czech Republic); Santava, Eva [Inst. of Physics ASCR, Prague (Czech Republic); Miliyanchuk, Khrystyna [Dept. of Inorganic Chemistry, Ivan Franko National Univ. of Lviv (Ukraine)

    2009-09-15

    Due to hydrogenation, the crystal structure of GdNiAl distorts from hexagonal to orthorhombic. The magnetic ordering temperature decreases dramatically and the magnetic entropy of this phase transition is in good agreement with theory. LuNiAl does not change its structure type, but it was found that the electronic contribution to specific heat decreases dramatically. None of the two phases loses its metallic character. (orig.)

  6. L’impegno al tempo della globalizzazione

    DEFF Research Database (Denmark)

    Cecchini, Leonardo

    al dibattito sui rapporti (di reciproca esclusione? complementarità?) tra postmoderno e impegno e sul carattere di quest’ultimo oggi (politico? sociale? etico? ecologico? altro?). Prendendo le mosse dalle discussioni suscitate dalla presa di posizione teorica dei Wu Ming, nel mio intervento vorrei...... Romanistica», 8/2011, pgg. 129-143. Wu Ming, New Italian Epic. Letteratura, sguardo oblique, ritorno al futuro, Torino, Einaudi, 2009....

  7. Del latín al rumano

    Directory of Open Access Journals (Sweden)

    Montes Giraldo José Joaquín

    2003-06-01

    Full Text Available

    Marius Sala, Del latín al rumano. Versión española de Valeria Neagu, París, Unión Latina - Bucarest, Univers enciclopedic, 2002. El texto se dirige al lector corriente, no especialista, y por eso el aparato técnico-bibliográfico no se presenta en el curso de la exposición.

  8. Hypermetabolism as a Risk Factor for ALS

    Science.gov (United States)

    2012-07-01

    hypermetabolism related to loss of TDP-43, an essential RNA binding protein implicated in ALS and Frontotemporal dementia ( FTD ), may contribute to disease...with Paget’s disease of bone and FTD (IBMPFD), it will also be interesting to test whether hypermetabolism may also be a risk factor in these...mouse strain confers leanness and protects from diet-induced obesity. Nat Genet 40(11):1354-1359, 2008. 3. Stone S, et al: TBC1D1 is a candidate

  9. Line shape diagnostics of Galactic 26Al

    CERN Document Server

    Kretschmer, K; Hartmann, D H

    2003-01-01

    The shape of the gamma-ray line from radioactive 26Al, at 1808.7 keV energy in the frame of the decaying isotope, is determined by its kinematics when it decays, typically 10^6 y after its ejection into the interstellar medium from its nucleosynthesis source. Three measurements of the line width exist: HEAO-C's 1982 value of (0+3) keV FWHM, the GRIS 1996 value of (5.4 +/- 1.3) keV FWHM, and the recent RHESSI value of (2.0 +/- 0.8) keV FWHM, suggesting either "cold", "hot", or "warm" 26Al in the ISM. We model the line width as expected from Galactic rotation, expanding supernova ejecta, and/or Wolf-Rayet winds, and predict a value below 1 keV (FWHM) with plausible assumptions about 26Al initial velocities and expansion history. Even though the recent RHESSI measurement reduces the need to explain a broad line corresponding to 540 km/s mean 26Al velocity through extreme assumptions about grain transport of 26Al or huge interstellar cavities, our results suggest that standard 26Al ejection models produce a line ...

  10. In Situ Fabrication of AlN Coating by Reactive Plasma Spraying of Al/AlN Powder

    Directory of Open Access Journals (Sweden)

    Mohammed Shahien

    2011-10-01

    Full Text Available Reactive plasma spraying is a promising technology for the in situ formation of aluminum nitride (AlN coatings. Recently, it became possible to fabricate cubic-AlN-(c-AlN based coatings through reactive plasma spraying of Al powder in an ambient atmosphere. However, it was difficult to fabricate a coating with high AlN content and suitable thickness due to the coalescence of the Al particles. In this study, the influence of using AlN additive (h-AlN to increase the AlN content of the coating and improve the reaction process was investigated. The simple mixing of Al and AlN powders was not suitable for fabricating AlN coatings through reactive plasma spraying. However, it was possible to prepare a homogenously mixed, agglomerated and dispersed Al/AlN mixture (which enabled in-flight interaction between the powder and the surrounding plasma by wet-mixing in a planetary mill. Increasing the AlN content in the mixture prevented coalescence and increased the nitride content gradually. Using 30 to 40 wt% AlN was sufficient to fabricate a thick (more than 200 µm AlN coating with high hardness (approximately 1000 Hv. The AlN additive prevented the coalescence of Al metal and enhanced post-deposition nitriding through N2 plasma irradiation by allowing the nitriding species in the plasma to impinge on a larger Al surface area. Using AlN as a feedstock additive was found to be a suitable method for fabricating AlN coatings by reactive plasma spraying. Moreover, the fabricated coatings consist of hexagonal (h-AlN, c-AlN (rock-salt and zinc-blend phases and certain oxides: aluminum oxynitride (Al5O6N, cubic sphalerite Al23O27N5 (ALON and Al2O3. The zinc-blend c-AlN and ALON phases were attributed to the transformation of the h-AlN feedstock during the reactive plasma spraying. Thus, the zinc-blend c-Al

  11. Corrosion behavior of solid solution (Ti,Al)N as a function of Al concentration

    Energy Technology Data Exchange (ETDEWEB)

    Larijani, M.M.; Seyedi, H. [Nuclear Science and Technology Research Institute, P. O. Box 31485-498, Karaj (Iran, Islamic Republic of); Manouchehrian, M. [Department of Physics, Science Faculty, South Tehran Branch, Islamic Azad University, Tehran (Iran, Islamic Republic of); Yari, M. [Engineering Department Materials, Science and Research Branch, Islamic Azad University, Tehran (Iran, Islamic Republic of)

    2012-08-15

    In this work, a series of (Ti, Al) N coatings with different Al contents were deposited on 304 stainless steel substrates by Hollow Cathode Discharge (HCD) method. The coatings were grown on 304 stainless steel substrates at 400 C. The coatings were characterized using energy dispersive X-ray spectroscopy (EDX), X-ray diffraction (XRD), atomic force microscopy (AFM), and microhardness test. The XRD confirmed the transition from TiN phase to (Ti, Al) N phase and then to AlN phase with increasing Al concentration in the solid solution. It was found that with increasing Al concentration the hardness of the coatings initially increased up to a maximum value of about 30 GPa at around 32 at.% of Al and then the coating hardness decreased rapidly with further increase of Al content (Al > 32 at.%). The potentiodynamic polarization analysis was carried out in 3.5 wt.% NaCl solutions to study the corrosion resistance of the coatings. From the corrosion test it can be inferred that the amount of Al atoms in the coatings plays an important role for reducing the corrosion. (Copyright copyright 2012 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim)

  12. Thermodynamics and surface properties of liquid Al-Ga and Al-Ge alloys

    Energy Technology Data Exchange (ETDEWEB)

    Anusionwu, B.C. [Abdus Salam Int. Centre for Theoretical Physics, Trieste (Italy); Federal University of Technology, Department of Physics, Owerri (Nigeria); Adebayo, G.A. [Abdus Salam Int. Centre for Theoretical Physics, Trieste (Italy); University of Agriculture, Department of Physics, Abeokuta (Nigeria); Madu, C.A. [Federal University of Technology, Department of Physics, Owerri (Nigeria)

    2009-11-15

    The surface properties of Al-Ga and Al-Ge liquid alloys have been theoretically investigated at a temperature of 1100 K and 1220 K respectively. For the Al-Ga system, the quasi chemical model for regular alloy and a model for phase segregating alloy systems were applied, while for the Al-Ge system the quasi chemical model for regular and compound forming binary alloys were applied. In the case of Al-Ga, the models for the regular alloys and that for the phase segregating alloys produced the same value of order energy and same values of thermodynamic and surface properties, while for the Al-Ge system, the model for the regular alloy reproduced better the thermodynamic properties of the alloy. The model for the compound forming systems showed a qualitative trend with the measured values of the thermodynamic properties of the Al-Ge alloy and suggests the presence of a weak complex of the form Al{sub 2}Ge{sub 3}. The surface concentrations for the alloys show that Ga manifests some level of surface segregation in Al-Ga liquid alloy while the surface concentration of Ge in Al-Ge liquid alloy showed a near Roultian behavior below 0.8 atomic fraction of Ge. (orig.)

  13. Thermodynamics and surface properties of liquid Al-Ga and Al-Ge alloys

    Science.gov (United States)

    Anusionwu, B. C.; Adebayo, G. A.; Madu, C. A.

    2009-11-01

    The surface properties of Al-Ga and Al-Ge liquid alloys have been theoretically investigated at a temperature of 1100 K and 1220 K respectively. For the Al-Ga system, the quasi chemical model for regular alloy and a model for phase segregating alloy systems were applied, while for the Al-Ge system the quasi chemical model for regular and compound forming binary alloys were applied. In the case of Al-Ga, the models for the regular alloys and that for the phase segregating alloys produced the same value of order energy and same values of thermodynamic and surface properties, while for the Al-Ge system, the model for the regular alloy reproduced better the thermodynamic properties of the alloy. The model for the compound forming systems showed a qualitative trend with the measured values of the thermodynamic properties of the Al-Ge alloy and suggests the presence of a weak complex of the form Al2Ge3. The surface concentrations for the alloys show that Ga manifests some level of surface segregation in Al-Ga liquid alloy while the surface concentration of Ge in Al-Ge liquid alloy showed a near Roultian behavior below 0.8 atomic fraction of Ge.

  14. Heterogeneous nucleation of solid Al from the melt by Al 3 Ti : Molecular dynamics simulations

    KAUST Repository

    Wang, Junsheng

    2010-10-27

    It has been known experimentally for some time that Al3 Ti is a powerful nucleant for the solidification of aluminum from the melt; however, a full microscopic understanding is still lacking. To develop this understanding, we have performed molecular dynamics simulations of the nucleation and early stages of growth using published embedded atom method potentials for Al-Ti, but modified by us to stabilize the D 022 structure. We discover that Al3 Ti can indeed be very effective in promoting the growth of solid Al but the manner in which growth takes place depends sensitively on the surface on which the Al nucleates. In particular, complete growth of solid Al from the liquid on the (001) and (110) surfaces of Al3 Ti occurs at a lower temperature than on the (112) surface. This anisotropy agrees with observations in previous experiments. We explain this observation in terms of interfacial energies. On the preferential (111) surface of Al the solid-liquid interfacial energy is highest while the solid-vacuum energy is lowest. Our simulations also show that the extent of ordering taking place in liquid Al close to the Al 3 Ti substrate above the melting point correlates well with the effectiveness of the substrate as a nucleant below the melting temperature: this could provide a computationally efficient scheme to identify good nucleants. © 2010 The American Physical Society.

  15. Al4C3 Hydration Thermochemical Analysis for Burned Carbon-containing Refractories with Al

    Institute of Scientific and Technical Information of China (English)

    YANG Ding'ao; YU Zhiming; FAN Liuwu

    2003-01-01

    In this paper, X-ray diffractogram analysis and SEM observation of Al4 C3 formed at high temperature from carbon-containing refractories with Al have been carried out.Aluminum added to carbon-containing refractories reacts with C(s)to form Al4 C3(s) gradually during heating from 600 ℃ to 1200℃.It is considered that the interlocked structure of Al4 C3 plate crystals promotes the outstanding increase of hot modulus of rupture of carbon-containing refractories with Al. The HMOR of carbon-containing refractories added with Al additive from 0 to 5wt% increases by 2.8 times being from 6.5MPa to 18.2MPa.After a thermochemical calculation for hydration reaction processes of Al4 C3 and H2O(g), the equilibrium partial pressure chart of H2O(g)in H2O-Al4C3-Al(OH)3 system vs various temperatures has been attained . The H2O (g) partial pressure in the air needed for the Al4 C3 hydration reaction is no more than 10~18 atm at the temperature below 120℃.It is considered that the burned carbon-containing refractories with Al is extremely easy to hydrate and the cracking of burned carbon-containing refractories is generated because that the hydration expansion is 2.11 times during transforming from Al4 C3 to Al(OH)3.The fundamental measure against hydration of the refractories is to insulate the refractories from H2O(g)by various means such as pitch impregnation or other sealing materials.

  16. Prinsip Pendidikan Karakter dalam Islam: Studi Komparasi Pemikiran Al-Ghazali dan Burhanuddin Al-Zarnuji

    Directory of Open Access Journals (Sweden)

    Agus Setiawan

    2014-06-01

    Full Text Available The impelemtantion of character education that is done by the Indonesian government recently has been provoked by al-Ghazali and Burhanuddin al-Zarnuji long ago. It was proven by some written thoughts of al-Ghazali and Burhanuddin al-Zarnuji. They were very famous scientists in their era and widely welknown as educational experts who produced some phenomenal thoughts many people use today. Al-Ghazali and Burhanuddin al-Zarnuji lived in the era of Bani Abbasiyah monarchy, but Both of them lived in different era. Al-Ghazali was born first, then Burhanuddin al-Zarnuji. They had different mazhab, al-Ghazali went to the traditional principle of mazhab Syafi’I and Burhanuddin al-Zarnuji went to the modern principle of mazhab Hanafi. The differences, of course, would create a different thought. Still, they might have a similar principle of Islamic character education. The thoughts of character education of al-Ghazali have been poured in the “Ayyuha Walad” focusing to the students’ character building characterized by religiously obedient, skillfull of general and religious knowledge, socially helpful, affectionate, generous, good citizen, and coloring the society. Some applicable thoughts of character education of Ayyuha al-Walad of al-Gazhali to the contemporary education are: the balance of the happiness purposes of the recent life and the life after death, qana’ah and tawakal, affectionate, caring other, patience, honesty, philanthrophy, social works, generousity, democratic, peace makers, and patriotic. Those characters are also directed in the islamic character education nowdays. So that, the thoughts of al-Ghazali positively and responsively are absorbed by the Indonesian government to build the national character widely implementing in the national curriculum based on the local wisdom and the Islamic values. In the end, the students are able to live happily based on the Islamic rules by implementing the good behavior. Similar to the al

  17. The association between chromaticity, phenolics, carotenoids, and in vitro antioxidant activity of frozen fruit pulp in Brazil: an application of chemometrics.

    Science.gov (United States)

    Zielinski, Acácio Antonio Ferreira; Ávila, Suelen; Ito, Vivian; Nogueira, Alessandro; Wosiacki, Gilvan; Haminiuk, Charles Windson Isidoro

    2014-04-01

    A total of 19 Brazilian frozen pulps from the following fruits: açai (Euterpe oleracea), blackberry (Rubus sp.), cajá (Spondias mombin), cashew (Anacardium occidentale), cocoa (Theobroma cacao), coconut (Cocos nucifera), grape (Vitis sp.), graviola (Annona muricata), guava (Psidium guajava), papaya (Carica papaya), peach (Prunus persica), pineapple (Ananas comosus), pineapple and mint (A. comosus and Mentha spicata), red fruits (Rubus sp. and Fragaria sp.), seriguela (Spondias purpurea), strawberry (Fragaria sp.), tamarind (Tamarindus indica), umbu (Spondias tuberosa), and yellow passion fruit (Passiflora edulis) were analyzed in terms of chromaticity, phenolic compounds, carotenoids, and in vitro antioxidant activity using ferric reducing antioxidant power (FRAP) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) assays. Data were processed using principal component analysis (PCA) and hierarchical cluster analysis (HCA). Antioxidant capacity was measured by DPPH and FRAP assays, which showed significant (P < 0.01) correlation with total phenolic compounds (r = 0.88 and 0.70, respectively), total flavonoids (r = 0.63 and 0.81, respectively), and total monomeric anthocyanins (r = 0.59 and 0.73, respectively). PCA explained 74.82% of total variance of data, and the separation into 3 groups in a scatter plot was verified. Three clusters also suggested by HCA, corroborated with PCA, in which cluster 3 was formed by strawberry, red fruits, blackberry, açaí, and grape pulps. This cluster showed the highest contents of total phenolic compounds, total flavonoids, and antioxidant activity.

  18. Diversity of Fusarium species and mycotoxins contaminating pineapple.

    Science.gov (United States)

    Stępień, Łukasz; Koczyk, Grzegorz; Waśkiewicz, Agnieszka

    2013-08-01

    Pineapple (Ananas comosus var. comosus) is an important perennial crop in tropical and subtropical areas. It may be infected by various Fusarium species, contaminating the plant material with mycotoxins. The aim of this study was to evaluate Fusarium species variability among the genotypes isolated from pineapple fruits displaying fungal infection symptoms and to evaluate their mycotoxigenic abilities. Forty-four isolates of ten Fusarium species were obtained from pineapple fruit samples: F. ananatum, F. concentricum, F. fujikuroi, F. guttiforme, F. incarnatum, F. oxysporum, F. polyphialidicum, F. proliferatum, F. temperatum and F. verticillioides. Fumonisins B1-B3, beauvericin (BEA) and moniliformin (MON) contents were quantified by high-performance liquid chromatography (HPLC) in pineapple fruit tissue. Fumonisins are likely the most dangerous metabolites present in fruit samples (the maximum FB1 content was 250 μg g(-1) in pineapple skin and 20 μg ml(-1) in juice fraction). In both fractions, BEA and MON were of minor significance. FUM1 and FUM8 genes were identified in F. fujikuroi, F. proliferatum, F. temperatum and F. verticillioides. Cyclic peptide synthase gene (esyn1 homologue) from the BEA biosynthetic pathway was identified in 40 isolates of eight species. Based on the gene-specific polymerase chain reaction (PCR) assays, none of the isolates tested were found to be able to produce trichothecenes or zearalenone.

  19. Microscopic Properties of Long-Period Ordering in Al-Rich TiAl Alloys

    Science.gov (United States)

    Hata, S.; Nakano, T.; Kuwano, N.; Itakura, M.; Matsumura, S.; Umakoshi, Y.

    2008-07-01

    The ordering mechanism of long-period superstructures (LPSs) in Al-rich TiAl alloys has been investigated by high-resolution transmission electron microscopy (HRTEM). The LPSs are classified in terms of arrangements of base clusters with different shapes and compositions formed in Ti-rich (002) layers of L10-TiAl matrix: square Ti4Al, fat rhombus Ti3Al, and lean rhombus Ti2Al type clusters. The HRTEM observations revealed that antiphase boundaries of long-range-ordered LPS domains and short-range-ordered microdomains are constructed by various space-filling arrangements of the base clusters. Such a microscopic property characterized by the base clusters and their arrangements is markedly analogous to that of the {left< {{text{1,1/2,0}}} rightrangle } * special-point ordering alloys such as Ni-Mo.

  20. Mechanochemical Synthesis and Rapid Consolidation of Nanocrystalline 3NiAl-Al2O3 Composites

    Directory of Open Access Journals (Sweden)

    In-Jin Shon

    2011-01-01

    Full Text Available Nanopowders of 3NiAl and Al2O3 were synthesized from 3NiO and 5Al powders by high-energy ball milling. Nanocrystalline Al2O3 reinforced composite was consolidated by high-frequency induction-heated sintering within 3 minutes from mechanochemically synthesized powders of Al2O3 and 3NiAl. The advantage of this process is that it allows very quick densification to near theoretical density and inhibition grain growth. Nanocrystalline materials have received much attention as advanced engineering materials with improved physical and mechanical properties. The relative density of the composite was 97%. The average Vickers hardness and fracture toughness values obtained were 804 kg/mm2 and 7.5 MPa⋅m1/2, respectively.

  1. Effects of Al(III) and nano-Al13 species on malate dehydrogenase activity.

    Science.gov (United States)

    Yang, Xiaodi; Cai, Ling; Peng, Yu; Li, Huihui; Chen, Rong Fu; Shen, Ren Fang

    2011-01-01

    The effects of different aluminum species on malate dehydrogenase (MDH) activity were investigated by monitoring amperometric i-t curves for the oxidation of NADH at low overpotential using a functionalized multi-wall nanotube (MWNT) modified glass carbon electrode (GCE). The results showed that Al(III) and Al(13) can activate the enzymatic activity of MDH, and the activation reaches maximum levels as the Al(III) and Al(13) concentration increase. Our study also found that the effects of Al(III) and Al(13) on the activity of MDH depended on the pH value and aluminum speciation. Electrochemical and circular dichroism spectra methods were applied to study the effects of nano-sized aluminum compounds on biomolecules.

  2. Comparison of Al electromigration in conventional Al alloy and W-plug contacts to silicon

    Science.gov (United States)

    Oates, A. S.; Martin, E. P.; Alugbin, D.; Nkansah, F.

    1993-06-01

    We examine the impact of the presence of a W plug on electromigration-induced voiding of the Al alloy layer of contacts to silicon. By comparing conventional Al alloy and W-plug contacts we show that the presence of the plug does not significantly improve the intrinsic susceptibility of contacts to Al voiding despite the elimination of the Al step coverage problem. Both the conventional Al alloy and W-plug contacts degrade by drift of the Al layer with a velocity that is not influenced by the contact structure. The sheet resistance of the underlayer material of multilayer conductors plays an important role in influencing failure times since it determines the rate of resistance increase due to electromigration.

  3. Kinetic Monte Carlo of transport processes in Al/AlOx/Au-layers: Impact of defects

    Science.gov (United States)

    Weiler, Benedikt; Haeberle, Tobias; Gagliardi, Alessio; Lugli, Paolo

    2016-09-01

    Ultrathin films of alumina were investigated by a compact kMC-model. Experimental jV-curves from Al/AlOx/Au-junctions with plasma- and thermal-grown AlOx were fitted by simulated ones. We found dominant defects at 2.3-2.5 eV below CBM for AlOx with an effective mass mox ∗= 0.35 m0 and a barrier EB ,A l /A l O x≈2.8 eV in agreement with literature. The parameterization is extended to varying defect levels, defect densities, injection barriers, effective masses and the thickness of AlOx. Thus, dominant charge transport processes and implications on the relevance of defects are derived and AlOx parameters are specified which are detrimental for the operation of devices.

  4. Effects of Al(III and Nano-Al13 Species on Malate Dehydrogenase Activity

    Directory of Open Access Journals (Sweden)

    Rong Fu Chen

    2011-05-01

    Full Text Available The effects of different aluminum species on malate dehydrogenase (MDH activity were investigated by monitoring amperometric i-t curves for the oxidation of NADH at low overpotential using a functionalized multi-wall nanotube (MWNT modified glass carbon electrode (GCE. The results showed that Al(III and Al13 can activate the enzymatic activity of MDH, and the activation reaches maximum levels as the Al(III and Al13 concentration increase. Our study also found that the effects of Al(III and Al13 on the activity of MDH depended on the pH value and aluminum speciation. Electrochemical and circular dichroism spectra methods were applied to study the effects of nano-sized aluminum compounds on biomolecules.

  5. Superconductivity in Al/Al{sub 2}O{sub 3} interface

    Energy Technology Data Exchange (ETDEWEB)

    Palnichenko, A.V., E-mail: paln@issp.ac.ru; Vyaselev, O.M.; Mazilkin, A.A.; Khasanov, S.S.

    2016-06-15

    Highlights: • Al/Al{sub 2}O{sub 3} interface was formed by surface oxidation of Al foil. • Magnetic susceptibility and electrical resistance of the interface was studied. • Superconductivity at ∼ 65 K of the Al/Al{sub 2}O{sub 3} interface was observed. • The superconducting interface is instable under normal conditions. - Abstract: Metastable superconductivity at T{sub c} ≈ 65 K has been observed in Al foil subjected to special oxidation process, according to the ac magnetic susceptibility and electrical resistance measurements. Comparison of the ac susceptibility and the dc magnetization measurements infers that the superconductivity arises within the interfacial granular layer formed during the oxidation process between metallic aluminum and its oxide.

  6. Protective Al2O3 scale formation on NbAl3-base alloys

    Science.gov (United States)

    Doychak, J.; Hebsur, M. G.

    1991-01-01

    The oxidation of NbAl3 with additions of Cr and Y was studied to determine the mechanisms of the beneficial effects of these elements upon oxidation. Cr additions to the binary NbAl3 alloy of up to 6.8 at. percent reduced the scale growth rates and promoted alpha-Al2O3 formation over much longer times relative to binary NbAl3. A major effect of Cr is to form a layer of AlNbCr at the metal/scale interface, which is inherently more oxidation-resistant than the matrix alloy in the long term. Yttrium additions to a Cr-containing alloy improved the scale growth rate and adherence and changed the scale microstructure to mimic that of a typical protective Al2O3 scale.

  7. {sup 27}Al NMR studies of NpPd{sub 5}Al{sub 2}

    Energy Technology Data Exchange (ETDEWEB)

    Chudo, H., E-mail: chudo.hiroyuki@jaea.go.j [Advanced Science Research Center, Japan Atomic Energy Agency, Tokai, Ibaraki 319-1195 (Japan); Sakai, H.; Tokunaga, Y.; Kambe, S. [Advanced Science Research Center, Japan Atomic Energy Agency, Tokai, Ibaraki 319-1195 (Japan); Aoki, D.; Homma, Y.; Shiokawa, Y. [Institute for Materials Research, Tohoku University, Oarai, Ibaraki 311-1313 (Japan); Haga, Y.; Ikeda, S.; Matsuda, T.D. [Advanced Science Research Center, Japan Atomic Energy Agency, Tokai, Ibaraki 319-1195 (Japan); Onuki, Y. [Advanced Science Research Center, Japan Atomic Energy Agency, Tokai, Ibaraki 319-1195 (Japan); Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043 (Japan); Yasuoka, H. [Advanced Science Research Center, Japan Atomic Energy Agency, Tokai, Ibaraki 319-1195 (Japan)

    2009-10-15

    We present {sup 27}Al NMR studies for a single crystal of the Np-based superconductor NpPd{sub 5}Al{sub 2}(T{sub c}=4.9K). We have observed a five-line {sup 27}Al NMR spectrum with a center line and four satellite lines separated by first-order nuclear quadrupole splittings. The Knight shift clearly drops below T{sub c}. The temperature dependence of the {sup 27}Al nuclear spin-lattice relaxation rate shows no coherence peak below T{sub c}, indicating that NpPd{sub 5}Al{sub 2} is an unconventional superconductor with an anisotropic gap. The analysis of the present NMR data provides evidence for strong-coupling d-wave superconductivity in NpPd{sub 5}Al{sub 2}.

  8. Preparation of TiAl3-Al composite coating by cold spraying

    Institute of Scientific and Technical Information of China (English)

    SHEN Li; KONG Ling-yan; XIONG Tian-ying; DU Hao; LI Tie-fan

    2009-01-01

    TiAl3-Al coating was deposited on orthorhombic Ti2AlNb alloy substrate by cold spraying with the mixture of pure Al and Ti as the feedstock powder at a fixed molar ratio of 3-1 when the spraying distance, gas temperature and gas pressure for the process were 10 mm, 250 ℃ and 1.8 MPa, respectively. The as-sprayed coating was then subjected to heat treatment at 630 ℃ in argon atmosphere for 5 h at a heating rate of 3 ℃/min and an argon gas flow rate of 40 mL/min. The obtained TiAl3-Al composite coating is about 212 μm with a density of 3.16 g/cm3 and a porosity of 14.69% in general. The microhardness and bonding strength for the composite coating are HV525 and 27.12 MPa.

  9. TEM characterization of oxidized AlGaAs/AlAs nonlinear optical waveguides

    Energy Technology Data Exchange (ETDEWEB)

    Guillotel, E; Langlois, C; Ghiglieno, F; Leo, G; Ricolleau, C, E-mail: cyril.langlois@univ-paris-diderot.f [Laboratoire Materiaux et Phenomenes Quantiques, CNRS-UMR 7162 Universite Paris Diderot - Paris 7 Case courrier 7021, 75205 Paris Cedex 13 (France)

    2010-09-29

    The internal interfaces of multilayer Al{sub x}Ga{sub 1-x}As/AlAs nonlinear optical waveguides are investigated by high-angle annular-dark-field and energy-filtered scanning transmission electron microscopy, before and after partial wet oxidation of AlAs layers. Via a simple phenomenological model, the corresponding roughness parameters allow prediction of the scattering-induced waveguide optical losses, which are in reasonable agreement with the experimental value of 0.5 cm{sup -1}. We also find that Al{sub x}Ga{sub 1-x}As layers adjacent to oxidized AlAs tend to be oxidized through the interfaces, even for low Al fraction, with typical oxidation depths of 9 nm for x = 0.7 and 2 nm for x = 0.

  10. 60Co gamma radiation effect on AlGaN/AlN/GaN HEMT devices

    Institute of Scientific and Technical Information of China (English)

    WANG Yan-Ping; LUO Yin-Hong; WANG Wei; ZHANG Ke-Ying; GUO Hong-Xia; GUO Xiao-Qiang; WANG Yuan-Ming

    2013-01-01

    The testing techniques and experimental methods of the 60Co gamma irradiation effect on AlGaN/AlN/GaN high electron mobility transistors (HEMTs) are established.The degradation of the electrical properties of the device under the actual radiation environment are analyzed theoretically,and studies of the total dose effects of gamma radiation on AlGaN/AlN/GaN HEMTs at three different radiation bias conditions are carried out.The degradation patterns of the main parameters of the AlGaN/AlN/GaN HEMTs at different doses are then investigated,and the device parameters that were sensitive to the gamma radiation induced damage and the total dose level induced device damage are obtained.

  11. Determination of Al Composition in Strained AlGaN Layers

    Institute of Scientific and Technical Information of China (English)

    ZHOU Sheng-Qiang; WU Ming-Fang; YAO Shu-De

    2005-01-01

    @@ Alx Ga1-x N/GaN heterostructures are grown on c-sapphire with the Al composition x from 0.2 to 0.4 and thicknesses from 20nm to 30nm. The lattice parameters a and c are determined from 2θ/ω scan. The AlGaN layers are found to be under tensile strain by using x-ray diffraction. Vegard's law induces a large deviation in Al composition determination by only considering the linear relationship between one lattice parameter (a or c) and Al composition. The accurate determination of Al composition is only possible with consideration of both the lattice parameters a and c, by assuming the tetragonal distortion in the AlGaN layer. Additionally, the results obtained from x-ray diffraction are verified by Rutherford backscattering.

  12. Cryptanalysis of Xinyu et al.'s NTRU-Lattice Based Key Exchange Protocol

    OpenAIRE

    Valluri, Maheswara Rao

    2016-01-01

    Xinyu et al. proposed a public key exchange protocol, which is based on the NTRU-lattice based cryptography. In this paper, we show how Xinyu et al.'s NTRU-KE: A lattice based key exchange protocol can be broken, under the assumption that a man-in-the middle attack is used for extracting private keys of users who participate in the key exchange protocol.

  13. Modified Mechanism of Eutectic Silicon in Al2O3/Al-Si Alloy

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    Al2O3/Al-Si alloy composite was manufactured by squeeze casting. The morphology of the eutectic silicon in the composite was observed by scanning electronic microscope (SEM), and the modified mechanism of eutectic silicon in the composite was approached. The alumina fiber in the composite can trigger twin during the growth of Al-Si eutectic and lead to the modification of eutectic silicon near the fiber.

  14. Two-Phase (TiAl+TiCrAl) Coating Alloys for Titanium Aluminides

    Science.gov (United States)

    Brady, Michael P. (Inventor); Smialek, James L. (Inventor); Brindley, William J. (Inventor)

    1998-01-01

    A coating for protecting titanium aluminide alloys, including the TiAl gamma + Ti3Al (alpha(sub 2)) class, from oxidative attack and interstitial embrittlement at temperatures up to at least 1000 C. is disclosed. This protective coating consists essentially of titanium, aluminum. and chromium in the following approximate atomic ratio: Ti(41.5-34.5)Al(49-53)Cr(9.5-12.5)

  15. Another Security Improvement over the Lin et al.'s E-voting Scheme

    Science.gov (United States)

    Asaar, Maryam Rajabzadeh; Mohajeri, Javad; Salmasizadeh, Mahmoud

    In 2003, Lin et al. have proposed an electronic voting scheme which can be utilized in large-scale elections, and claimed it detects double voting. But in this paper, by presenting an attack, we show that voters can successfully vote more than once without being detected. Hence, we propose a new modified scheme based on the Lin et al.'s scheme with the same efficiency to solve this weakness and analyze its security.

  16. Compressive properties of a reaction milled NiAl-AlN composite

    OpenAIRE

    Whittenberger, J. Daniel; Arzt, Eduard; Luton, Michael J.

    1990-01-01

    Cryomilling (high intensity mechanical ball milling in a liquid nitrogen bath) of the B2 crystal structure nickel aluminide leads to a NiAl composite containing about 10 vol. % of AlN particles. This is the result of a reaction milling process, where nitrogen incorporated into the matrix during cryomilling reacts with Al during subsequent thermomechanical processing to form the composite. Compressive testing at 1300 K of such materials densified by 1505 K extrusion or isostatic pressing at 13...

  17. Generation and evolution of nanoscale AlP and Al{sub 13}Fe{sub 4} particles in Al-Fe-P system

    Energy Technology Data Exchange (ETDEWEB)

    Qiao, Huan; Gao, Tong; Zhu, Xiangzhen; Wu, Yuying; Qian, Zhao; Liu, Xiangfa, E-mail: xfliu@sdu.edu.cn

    2015-02-15

    Highlights: • Diffusion and gradual solid reactions between Al and Fe{sub x}P phases in Al-Fe-P alloy were investigated. • Nanoscale AlP clusters are in-situ generated and evolve during the whole process. • This novel Al-Fe-P alloy has an excellent low-temperature refining performance on hypereutectic Al-Si alloy. - Abstract: In this paper, the gradual solid reactions between Al and Fe{sub x}P phases in Al-Fe-P alloy were investigated. The results show that the whole reaction process undergoes four main stages: the diffusion of Al atom, the generation of (Al, Fe, P) intermediate compound, the precipitation of nano AlP and Al{sub 13}Fe{sub 4} clusters and their growth to submicron particles. The microstructure of Fe-P particles evolves from the “egg-type”, the “sponge-type” to the “sesame-cake” structure. AlP and Al{sub 13}Fe{sub 4} nano phases have in-situ generated and evolved during the whole process. The gradual reaction mechanism has been discussed. Furthermore, a novel Al-Fe-P alloy which contains (Al, Fe, P) intermediate compounds and nano AlP particles has been synthesized and its low-temperature refining performance on A390 alloy has also been investigated.

  18. Microstructure and thermal stability of mechanically alloyed Al3Ti/Al alloy

    Institute of Scientific and Technical Information of China (English)

    林建国; 魏浩岩; 黄正

    2001-01-01

    The microstructure stability of Al3Ti/Al alloy prepared by mechanical alloying (MA) was investigated in the simulating environment in which they may be used. The results show that the MA alloy possesses fine microstructure (the grain size is about 0.5  μm). After cycling loaded followed by heat exposure at 350  ℃ for 24  h, no microstructure coarsening of the alloy occurred, which means that the Al3Ti/Al alloy behaves good microstructure stability at high temperature. The compression yield strength of the alloy reaches up to 247  MPa at 350  ℃.

  19. Hydrolyzed Al(III) clusters: speciation stability of nano-Al13.

    Science.gov (United States)

    Wang, Dongsheng; Wang, Shuifeng; Huang, Chihpin; Chow, Christopher W K

    2011-01-01

    Pure nano-Al13 and aggregates at various concentrations were prepared to examine the particle size effect of coagulation with inorganic polymer flocculant. The property and stability of various species formed were characterized using Infrared, 27Al-NMR, photo correlation spectroscopy (PCS), and Ferron assay. Results showed that concentration and temperature exhibited different roles on the stability of Al13. The quantity of Alb species analyzed by ferron assay in the initial aging period corresponded well with that of Al13, which has been confirmed in a dimension range of 1-2 nm by PCS. Al13 solutions at high concentrations (0.5-2.11 mol/L) were observed to undergo further aggregation with aging. The aggregates with a wide particle size distribution would contribute to the disappeared/decreased Al13 basis on the 27Al-NMR spectrum, whereas a part of Al13 would still remain as Alb. At low concentrations, Al13 solution was quite stable at normal temperature, but lost its stability quickly when heating to 90 degrees C.

  20. Synthesis of core-shell AlOOH hollow nanospheres by reacting Al nanoparticles with water

    Science.gov (United States)

    Lozhkomoev, A. S.; Glazkova, E. A.; Bakina, O. V.; Lerner, M. I.; Gotman, I.; Gutmanas, E. Y.; Kazantsev, S. O.; Psakhie, S. G.

    2016-05-01

    A novel route for the synthesis of boehmite nanospheres with a hollow core and the shell composed of highly crumpled AlOOH nanosheets by oxidizing Al nanopowder in pure water under mild processing conditions is described. The stepwise events of Al transformation into boehmite are followed by monitoring the pH in the reaction medium. A mechanism of formation of hollow AlOOH nanospheres with a well-defined shape and crystallinity is proposed which includes the hydration of the Al oxide passivation layer, local corrosion of metallic Al accompanied by hydrogen evolution, the rupture of the protective layer, the dissolution of Al from the particle interior and the deposition of AlOOH nanosheets on the outer surface. In contrast to previously reported methods of boehmite nanoparticle synthesis, the proposed method is simple, and environmentally friendly and allows the generation of hydrogen gas as a by-product. Due to their high surface area and high, slit-shaped nanoporosity, the synthesized AlOOH nanostructures hold promise for the development of more effective catalysts, adsorbents, vaccines and drug carriers.

  1. Hydrolyzed Al(Ⅲ) clusters: Speciation stability of nano-Al13

    Institute of Scientific and Technical Information of China (English)

    Dongsheng Wang; Shuifeng Wang; Chihpin Huang; Christopher W K. Chow

    2011-01-01

    Pure nano-Al13 and aggregates at various concentrations were prepared to examine the particle size effect of coagulation with inorganic polymer flocculant.The property and stability of various species formed were characterized using Infrared, 27Al-NMR,photo correlation spectroscopy (PCS), and Ferron assay.Results showed that concentration and temperature exhibited different roles on the stability of Al13.The quantity of Alb species analyzed by ferron assay in the initial aging period corresponded well with that of Al13, which has been confirmed in a dimension range of 1-2 nm by PCS.Al13 solutions at high concentrations (0.5-2.11 mol/L) were observed to undergo further aggregation with aging.The aggregates with a wide particle size distribution would contribute to the disappeared/decreased Al13 basis on the 27Al-NMR spectrum, whereas a part of Al13 would still remain as Alb.At low concentrations,Al13 solution was quite stable at normal temperature, but lost its stability quickly when heating to 90℃.

  2. CASTABILITY OF Al-Li-Mg AND Al-Li-Cu-Mg ALLOYS

    OpenAIRE

    1987-01-01

    The objective of the present work is to study the casting characteristics of various Al-Li alloys, which include fluidity and strengths of the alloys and their interaction with cast molds. Materials investigated are Al-Li-Mg and Al-Li-Cu-Mg alloys with Li content of 2.5 wt%. The results show that sand molds with resin binders are good for Al-Li casting. Ceramic coatings can further reduce the metal-mold interactions. However, the permeability is also reduced by coating. The fluidity of Li-bea...

  3. Growth of CdTe: Al films; Crecimiento de peliculas de CdTe: Al

    Energy Technology Data Exchange (ETDEWEB)

    Gonzalez A, M.; Zapata T, M. [CICATA-IPN, 89600 Altamira, Tamaulipas (Mexico); Melendez L, M. [CINVESTAV-IPN, A.P. 14-740, 07000 Mexico D.F. (Mexico); Pena, J.L. [CINVESTAV-IPN, A.P. 73 Cordemex, 97310 Merida, Yucatan (Mexico)

    2006-07-01

    CdTe: AI films were grown by the close space vapor transport technique combined with free evaporation (CSVT-FE). The Aluminum (Al) evaporation was made by two kinds of sources: one made of graphite and the other of tantalum. The films were deposited on glass substrates. The Al source temperature was varied maintaining the CdTe source temperature fixed as well as the substrate temperature. The films were characterized by x-ray energy dispersive analysis (EDAX), x-ray diffraction and optical transmission. The results showed for the films grown with the graphite source for Al evaporation, the Al did not incorporate in the CdTe matrix, at least to the level of EDAX sensitivity; they maintained the same crystal structure and band gap. For the samples grown with the tantalum source, we were able to incorporate the Al. The x-ray diffraction patterns show that the films have a crystal structure that depends on Al concentration. They were cubic up to 2.16 at. % Al concentration; for 19.65 at. % we found a mixed phase; for Al concentration higher than 21 at. % the films were amorphous. For samples with cubic structure it was found that the lattice parameter decreases and the band gap increases with Al concentration. (Author)

  4. Study on Mg/Al Weld Seam Based on Zn–Mg–Al Ternary Alloy

    Directory of Open Access Journals (Sweden)

    Liming Liu

    2014-02-01

    Full Text Available Based on the idea of alloying welding seams, a series of Zn–xAl filler metals was calculated and designed for joining Mg/Al dissimilar metals by gas tungsten arc (GTA welding. An infrared thermography system was used to measure the temperature of the welding pool during the welding process to investigate the solidification process. It was found that the mechanical properties of the welded joints were improved with the increasing of the Al content in the Zn–xAl filler metals, and when Zn–30Al was used as the filler metal, the ultimate tensile strength could reach a maximum of 120 MPa. The reason for the average tensile strength of the joint increasing was that the weak zone of the joint using Zn–30Al filler metal was generated primarily by α-Al instead of MgZn2. When Zn–40Al was used as the filler metal, a new transition zone, about 20 μm-wide, appeared in the edge of the fusion zone near the Mg base metal. Due to the transition zones consisting of MgZn2- and Al-based solid solution, the mechanical property of the joints was deteriorated.

  5. Natural incidence of fusariose in pineapple fruits ‘Gold’

    Directory of Open Access Journals (Sweden)

    Luciana Cordeiro do Nascimento

    2009-12-01

    Full Text Available Pineapple (Ananas comosus L. Merr is a species of the family Bromeliaceae, consumed throughout the world and considered of great importance in areas of fruit production in Brazil. Some problems postharvest has contributed to disable the marketing these fruits so that there is a significant loss of quality of final product and, consequently, contributing to the reduction of postharvest life of fruit. The objective of this study was to evaluate the natural occurrence and to determine the incidence of Fusariose (Fusarium subglutinans f.sp. ananas in fruit of pineapple ‘Gold’ level of maturity harvested commercial. The fruits were harvested in the commercial area in the municipality of Mamanguape, state of Paraiba, Brazil, transported to the Laboratory of phytopathology of the Centro de Ciências Agrárias da Universidade Federal da Paraíba - CCA/UFPB, stored a temperature (25 ± 4 °C, UR 72% ±8 for 10 days without treatment postharvest. At the end of the storage period, there was the incidence of F. subglutinans in 29% of the fruits.

  6. The pineapple genome and the evolution of CAM photosynthesis.

    Science.gov (United States)

    Ming, Ray; VanBuren, Robert; Wai, Ching Man; Tang, Haibao; Schatz, Michael C; Bowers, John E; Lyons, Eric; Wang, Ming-Li; Chen, Jung; Biggers, Eric; Zhang, Jisen; Huang, Lixian; Zhang, Lingmao; Miao, Wenjing; Zhang, Jian; Ye, Zhangyao; Miao, Chenyong; Lin, Zhicong; Wang, Hao; Zhou, Hongye; Yim, Won C; Priest, Henry D; Zheng, Chunfang; Woodhouse, Margaret; Edger, Patrick P; Guyot, Romain; Guo, Hao-Bo; Guo, Hong; Zheng, Guangyong; Singh, Ratnesh; Sharma, Anupma; Min, Xiangjia; Zheng, Yun; Lee, Hayan; Gurtowski, James; Sedlazeck, Fritz J; Harkess, Alex; McKain, Michael R; Liao, Zhenyang; Fang, Jingping; Liu, Juan; Zhang, Xiaodan; Zhang, Qing; Hu, Weichang; Qin, Yuan; Wang, Kai; Chen, Li-Yu; Shirley, Neil; Lin, Yann-Rong; Liu, Li-Yu; Hernandez, Alvaro G; Wright, Chris L; Bulone, Vincent; Tuskan, Gerald A; Heath, Katy; Zee, Francis; Moore, Paul H; Sunkar, Ramanjulu; Leebens-Mack, James H; Mockler, Todd; Bennetzen, Jeffrey L; Freeling, Michael; Sankoff, David; Paterson, Andrew H; Zhu, Xinguang; Yang, Xiaohan; Smith, J Andrew C; Cushman, John C; Paull, Robert E; Yu, Qingyi

    2015-12-01

    Pineapple (Ananas comosus (L.) Merr.) is the most economically valuable crop possessing crassulacean acid metabolism (CAM), a photosynthetic carbon assimilation pathway with high water-use efficiency, and the second most important tropical fruit. We sequenced the genomes of pineapple varieties F153 and MD2 and a wild pineapple relative, Ananas bracteatus accession CB5. The pineapple genome has one fewer ancient whole-genome duplication event than sequenced grass genomes and a conserved karyotype with seven chromosomes from before the ρ duplication event. The pineapple lineage has transitioned from C3 photosynthesis to CAM, with CAM-related genes exhibiting a diel expression pattern in photosynthetic tissues. CAM pathway genes were enriched with cis-regulatory elements associated with the regulation of circadian clock genes, providing the first cis-regulatory link between CAM and circadian clock regulation. Pineapple CAM photosynthesis evolved by the reconfiguration of pathways in C3 plants, through the regulatory neofunctionalization of preexisting genes and not through the acquisition of neofunctionalized genes via whole-genome or tandem gene duplication.

  7. Direct laser cooling Al+ ions optical clocks

    CERN Document Server

    Zhang, J; Luo, J; Lu, Z H

    2016-01-01

    Al$^+$ ions optical clock is a very promising optical frequency standard candidate due to its extremely small blackbody radiation shift. It has been successfully demonstrated with indirect cooled, quantum-logic-based spectroscopy technique. Its accuracy is limited by second-order Doppler shift, and its stability is limited by the number of ions that can be probed in quantum logic processing. We propose a direct laser cooling scheme of Al$^+$ ions optical clocks where both the stability and accuracy of the clocks are greatly improved. In the proposed scheme, two Al$^+$ ions traps are utilized. The first trap is used to trap a large number of Al$^+$ ions to improve the stability of the clock laser, while the second trap is used to trap a single Al$^+$ ions to provide the ultimate accuracy. Both traps are cooled with a continuous wave 167 nm laser. The expected clock laser stability can reach $9.0\\times10^{-17}/\\sqrt{\\tau}$. For the second trap, in addition to 167 nm laser Doppler cooling, a second stage pulsed ...

  8. MOSQUE ARCHITECTURE AS A SUSTAINABLE BUILDING IN URBAN (Case Study: Al Markas Al Islamic Mosque Makassar

    Directory of Open Access Journals (Sweden)

    Imriyanti Imriyanti

    2014-02-01

    Full Text Available Abstract Mosque Architecture is a part of Islamic architecture. Mosque should be fused with elements of nature because its function as a place to pray to God who created the universe. Al Markas Al Islamic Makassar mosque is used as a center of Islamic aspirations of the people and the government to accommodate all the activities of moslems in Makassar. Through this function, the provision of Al Markas Al Islamic mosque began from the desire of Islamic societies in Makassar, which wants the Islamic center to be equipped with facilities and infrastructure as well as architectural, monumental buildings and structures that can be last for hundreds of years (continuous, blend with the environment, and also can be used by the public. Having regard to the function of a mosque especially at the Al Markas Al Islamic Makassar mosque, then the problem that arise is how the Al Markas Al Islamic mosque can be survived/sustainable  in order  to function  as a place  of worship  and  as a center  of Islam.  This research  is a descriptive qualitative research that is trying to generate data in the form of systematic and accurate picture of the object of study. The sustaiprophetlity  of Al Markas AL Islamic mosque can be known through the concept of Islam  in accordance with the view of Islam as well as the sustaiprophetlity of the buildings that seen in the spatial pattern of the mosque, appearance, natural lighting, natural ventilation, and user behavior of the mosque. Keywords: mosque architecture, sustaiprophetlity, view of Islam     Abstrak Arsitektur masjid merupakan bagian dari arsitektur Islam. Bangunan masjid sebaiknya dapat menyatu dengan unsur alam karena masjid difungsikan sebagai wadah dalam bersujud/sembahyang  kepada Allah SWT yang menciptakan alam semesta ini. Masjid Al Markas Al Islamic Makassar merupakan kompleks kegiatan Islam yang digunakan sebagai pusat aspirasi masyarakat  dan pemerintahan  yang dapat menampung

  9. ‘Uluww al-Isnad dalam Periwayatan Hadis Abu Muhammad al-Husain bin Mas‘ud al-Baghawi

    Directory of Open Access Journals (Sweden)

    Romlah Abubakar Askar

    2016-05-01

    Full Text Available Abu Muhammad al-Husayn Ibnu Mas’ud al-Baghawi (450-516H was one of the leading scholars in Tafsir (Quran exegesis, hadith (prophetic tradition, and fiqh (Islamic legal jurisprudence in his era. He was categorized as muta’akhirūn (scholars who came later on and was instrumental in the development of these three disciplines. In the field of exegesis, he was among the scholars who used al-Ma’tsūr, (commentary stylist, relying on the verses of the Qur’an, hadith, and the statements from the followers of the tabi’īn (the followers of the followers. This can be found in his recorded work Ma’ālim al Tanzīl. In the field of hadith, he preserved the narration method and presenting a new approach by merging some patterns of hadith narration from several books of hadith in his work known as ṭarīqah al-Jam’u (combination [amalgamation] method. This pattern will not occur unless there is the existence of other transmission lines as a second chain of narration used to produce ‘uluww al-Isnād (the noble sanad or nuzūl al-Isnād (descent sanad. It is recorded in his works: al-Jam’u baina al-Ṣaḥīḥain, Sharḥ al-Sunnah and Maṣābiḥ al-Sunnah. In the field of Jurisprudence, he was amongst those mujtahidin from the Shafiee school of thought, recorded in his Majmū’ ‘al-Fatāwā, al-Kifāyah fī al-Furū’ and Kitāb al-Tahdzīb. This paper aims to examine one of al-Baghawi’s skills in sanad collection which is the uluww al-isnād and nuzūl al-isnād, recorded from his two works Ma’ālim al-Tanzīl and Syarḥ al-Sunnah. The quantitative, qualitative and comparative approach has been used in this study to give an authentic description of the terms ‘uluww al-isnād, nuzūl al-isnād from al-Baghawi. Studies have found there are many examples of uluww al-Isnād and nuzūl al-isnāds contained in the patterns of al-Baghawi’s chain of narration towards the Ṣaḥīḥayn of al-Bukhari and Muslim as the mustakhrij

  10. Autologous Stem Cell Transplant for AL Amyloidosis

    Directory of Open Access Journals (Sweden)

    Vivek Roy

    2012-01-01

    Full Text Available AL amyloidosis is caused by clonal plasma cells that produce immunoglobulin light chains which misfold and get deposited as amyloid fibrils. Therapy directed against the plasma cell clone leads to clinical benefit. Melphalan and corticosteroids have been the mainstay of treatment for a number of years and the recent availability of other effective agents (IMiDs and proteasome inhibitors has increased treatment options. Autologous stem cell transplant (ASCT has been used in the treatment of AL amyloidosis for many years. It is associated with high rates of hematologic response and improvement in organ function. However, transplant carries considerable risks. Careful patient selection is important to minimize transplant related morbidity and mortality and ensure optimal patient outcomes. As newer more affective therapies become available the role and timing of ASCT in the overall treatment strategy of AL amyloidosis will need to be continually reassessed.

  11. Analyse de Viaje al pasado: los aztecas

    OpenAIRE

    Denis Lucchinacci

    2005-01-01

    1. Présentation du logiciel Viaje al pasado: los aztecas (el perfecto simple y el imperfecto en la narración y la descripción) a été conçu par Annie Desnoyers, linguiste à l'université de Montréal et Matilde Asencio, enseignante au Cégep du Vieux Montréal (Collège d'enseignement général et professionnel []), pour un public d'étudiants d'Espagnol Langue Étrangère (ELE). Disponible en deux versions - en ligne et sous forme de cédérom -, il s'agit d'un exerciseur destiné à la pratique du passé e...

  12. Analyse de Viaje al pasado: los aztecas

    Directory of Open Access Journals (Sweden)

    Denis Lucchinacci

    2005-11-01

    Full Text Available 1. Présentation du logiciel Viaje al pasado: los aztecas (el perfecto simple y el imperfecto en la narración y la descripción a été conçu par Annie Desnoyers, linguiste à l'université de Montréal et Matilde Asencio, enseignante au Cégep du Vieux Montréal (Collège d'enseignement général et professionnel [], pour un public d'étudiants d'Espagnol Langue Étrangère (ELE. Disponible en deux versions - en ligne et sous forme de cédérom -, il s'agit d'un exerciseur destiné à la pratique du passé e...

  13. Thermal Properties of Al-50%Si Alloys

    Institute of Scientific and Technical Information of China (English)

    Akio Nishimoto; Katsuya Akamatsu; Kazuyoshi Nakao; Kazuo Ichii

    2004-01-01

    In order to prepare a hypereutectic Al-Si alloy with low coefficients of thermal expansion (CTE), Al-50was produced by powder metallurgy (P/M) and ingot metallurgy (I/M). P/M specimen was prepared by mechanical alloying(MA) and pulsed electric-current sintering (PECS). The microstructures of specimens were characterized by optical microscopy and scanning electron microscopy (SEM) equipped with energy dispersive X-ray spectroscopy (EDS). Vickers microhardness and CTE measurements were performed. The grains in the P/M specimen were refined with increasing MA time. Primary Si and eutectic Si in the I/M specimen were remarkably refined by adding minute amounts of Sr. The CTE of P/M and I/M specimens were estimated as 7.8×10-6 and 10.7×10-6, respectively. These values were as same as a CTE of Al2O3 ceramics.

  14. [Al-Biruni--a universal scientist].

    Science.gov (United States)

    Kujundzić, E; Masić, I

    1999-01-01

    Al-Biruni's was of Persian descent. He was born in Horesmiya and had studied mathematics, history and medicine. Acquiring knowledge from these sciences, he wrote an outstanding work on chronology of several nations and devoted it to Ziyarit ruler Kabus. He made a chronological overview of calendars from many nations, including Persians, Greeks, Egyptians, Jews, Melkitian and Nestorian Christians, Sabeyaans as well as the old Arabs. Data presented in the work, according to the later authors, were taken from very reliable sources. He was contemporary of Ibn-Sina, and thanks to their friendship, they have discussed very much miscellaneous topics. He belonged to the group of scholars, taken by Gaznevian Soultan Mahmud to a long journey to India. Afterwards Al-Biruni wrote and published detailed work "Description of India"--a work on cultural history of India. Due to excellent abilities of Al-Biruni as a philosopher and scholar, there are still significant and reliable notes about buddhistic philosophy, structure of castes and Brahmans' life style. In this Al-Biruni's masterpiece, there are many comparative analysis of Suffism and certain Indian philosophical methods. Al-Biruni's most important work is "Pharmacopoeia"--"Kitab al-saydala", which brilliantly describes all medicaments. This work has been published in many languages. He also wrote few works on astronomy and astrology. In those works he has explained some astrological events through scientific approach in a such peculiar way that nobody has ever explained before. He was also interested in sciences like geology, mineralology, geography, mathematics, psychology and many others.

  15. Kritik Terhadap Pemikiran Tasawuf Al-Raniri

    Directory of Open Access Journals (Sweden)

    Syaifan Nur

    2013-12-01

    Full Text Available Abstract : Al-Raniri is often positioned by some scholars as “superior” figure, which is contrasted to Hamzah Fansuri and Syamsuddin Sumaterani, and that is why they predicate him as one of pioneer of Islamic reformer in Malay- Indonesian archipelago. Anti-wujūdiyyah Sufism campaign by al-Raniri quite successful to some extent to amputate the rate of development of philosophical Sufism was brought by Fansuri and Sumaterani in the one hand, and strike off the nature of moral Sufism in the other, redeemed by bloodshed. This article discussed the “superiority” of al-Raniri’s thought of Sufism, viewed from a broader spectrum, either in relation to problems of faith or theology and politics surrounding the circumstance of his time.Keywords : Philosophical tasawuf, neo-sufism, panteism, tajalli, God’s attributeAbstrak : Al-Raniri oleh banyak kalangan seringkali diposisikan sebagai tokoh “superior” yang diperlawankan dengan Hamzah Fansuri dan Syamsuddin Sumaterani, dan itu sebabnya para ahli memberinya predikat sebagai seorang pelopor pembaru (mujaddid Islam di Nusantara. Anti tasawuf wujūdiyyah yang dikampanyekan oleh al-Raniri cukup berhasil mengamputasi laju perkembangan tasawuf falsafi yang dibawa oleh Fansuri dan Sumaterani di satu sisi, dan melancarkan laju corak tasawuf akhlaki di sisi lain, yang ditebus dengan cara pertumpahan darah. Tulisan ini mendiskusikan “superioritas” pemikiran tasawuf al-Raniri tersebut, dilihat dari spektrum yang lebih luas, baik menyangkut persoalan keyakinan atau teologi maupun politik yang mengitari situasi zamannya.Kata kunci : Tasawuf falsafi , neo-sufisme, panteisme, tajalli, sifat Tuhan

  16. Fabrication of Al-Al3Ti/Ti3Al Functionally Graded Materials under a Centrifugal Force

    Directory of Open Access Journals (Sweden)

    Yoshimi Watanabe

    2010-09-01

    Full Text Available Fabrication of Al-Al3Ti functionally graded materials (FGMs under the centrifugal force has recently attracted some attention. The controlled compositional gradient of the fabricated FGMs, the low cost of the process, and the good mold filling, are the main advantages of the centrifugal method (CM. Using the conventional CM techniques such as the centrifugal solid-particle method and centrifugal in-situ method, FGMs rings with gradually distributed properties could be achieved. As a more practical choice, the centrifugal mixed-powder method (CMPM was recently proposed to obtain FGMs containing nano-particles selectively dispersed in the outer surface of the fabricated parts. However, if a control of the particles morphology, compound formulas or sizes, is desired, another CM technique is favored. As a development of CMPM, our novel reaction centrifugal mixed-powder method (RCMPM has been presented. Using RCMPM, Al‑Al3Ti/Ti3Al FGMs with good surface properties and temperature controlled compositional gradient could be achieved. In this short review, this novel method will be discussed in detail and the effect of RCMPM processing temperature on the reinforcement particles morphology, size and distribution through the fabricated samples, will be reviewed.

  17. Total energy, equation of state and bulk modulus of AlP, AlAs and AlSb semiconductors

    Indian Academy of Sciences (India)

    A R Jivani; H J Trivedi; P J Gajjar; A R Jani

    2005-01-01

    Recently proposed model potential which combines both linear and quadratic types of interactions is employed for the investigation of some properties like the total energy, equation of state and bulk modulus of AlP, AlAs and AlSb semiconductor compounds using higher-order perturbation theory. The model potential parameter is determined using zero pressure condition. The ratio of the covalent bonding term E cov to the second-order term 2 is 6.77% to 11.85% which shows that contribution from higher order terms are important for zinc-blende-type crystals. The calculated numerical results of the total energy, energy band gap at Jones-zone face and bulk modulus of these compounds are in good agreement with the experimental data and found much better than other such theoretical findings. We have also studied pressure–volume relations of these compounds. The present study is carried out using six different screening functions along with latest screening function proposed by Sarkar et al. It is found from the present study that effect of exchange and correlation is clearly distinguishable.

  18. Semantische Merkmale als Mittel der Textkonstitution

    Directory of Open Access Journals (Sweden)

    Stojan Bračič

    2008-12-01

    Full Text Available Im folgenden Beitrag sollen semantische Relationen unter die Lupe genommen werden, die im text dazu beitragen, dass einzelne Textkomponenten miteinander verknüpft werden. Dabei geht es hauptsächlich um Relationen, die nicht satzthematisch sind - diese sind nämlich als textuelle Koreferenzketten ausreichend untersucht worden (vgl. u.a. Bračič/Fix/Greule 2007 - sondern um jene Relationen, die als Prädikationen einzelner textgrammatischer Einheiten (Sätze vorkommen und ebenfalls gewisse geordnete Strukturierungen aufweisen.

  19. Aspectos biopsicosociales asociados al embarazo adolescente

    OpenAIRE

    2012-01-01

    Introducción: El embarazo en la adolescencia, es un evento inesperado, sorpresivo. Este artículo establece la importancia de identificar los aspectos biopsicosociales asociados al embarazo adolescente en las embarazadas asistentes al programa de Control Prenatal de una Empresa Social del Estado de nivel 1 en Montería en 2009, para comprender las actitudes y los comportamientos asociados a esta etapa tan temprana. Materiales y Métodos: Es un estudio descriptivo, cuantitativo, de corte transver...

  20. The wobbler mouse, an ALS animal model

    DEFF Research Database (Denmark)

    Moser, Jakob Maximilian; Bigini, Paolo; Schmitt-John, Thomas

    2013-01-01

    This review article is focused on the research progress made utilizing the wobbler mouse as animal model for human motor neuron diseases, especially the amyotrophic lateral sclerosis (ALS). The wobbler mouse develops progressive degeneration of upper and lower motor neurons and shows striking...... the disease mechanism and testing various therapeutic approaches and discuss the relevance of these advances for human ALS. The identification of the causative mutation linking the wobbler mutation to a vesicle transport factor and the research focussed on the cellular basis and the therapeutic treatment...

  1. Simulations of the premelting of Al(110)

    DEFF Research Database (Denmark)

    Stoltze, Per

    1990-01-01

    The premelting of Al(110) is studied by molecular dynamics simulations using the potential for Al derived from effective medium theory. We find a progressive disordering of the surface region as the bulk melting point is approached. This disorder is mediated by the formation of adatom–vacancy pairs....... Based on the calculated radial distribution function and the analytical expression for the energy, it is demonstrated that the thermodynamics of disorder and melting is reflected in the single particle properties by a decrease in the number of nearest neighbors. The Debye temperature for the surface...

  2. Del Derecho laboral al Derecho del trabajo

    OpenAIRE

    Jaramillo Jassir, Ivan Daniel

    2011-01-01

    El Derecho Laboral hijo de la revolución industrial, calificado como el acontecimiento jurídico del siglo XX, reduce su ámbito de estudio al trabajo subordinado. Los cambios del sistema de producción superan la centralidad del contrato de trabajo subordinado a tiempo indeterminado, situación que demanda ampliación del ámbito de aplicación subjetivo y objetivo de la materia, dando paso al Derecho del Trabajo que se ocupa del trabajo en todas sus modalidades como fuente de ingreso para satisfac...

  3. Nuestra labor de mirar al otro

    OpenAIRE

    Campos Saborío, Natalia

    2016-01-01

    El estudio etnográfico que dio origen al libro que hoy presentamos, se tituló la "Etnografía del cambio educativo en el ámbito de la investigación social". Una de sus misiones fue buscar al otro, entendiéndose el otro, de aquí en adelante como director, directora, docentes, niños y niñas y padres de familia de la escuela #1, como la llamamos en la investigación antes citada.

  4. Magnetic Properties of NdAl2

    DEFF Research Database (Denmark)

    Bak, P.

    1974-01-01

    The magnetic properties of NdAl2 are calculated using a Hamiltonian including crystal-field and isotropic exchange interaction terms. A two-dimensional mean-field theory is evaluated to calculate single-crystal magnetization curves. It is shown that the magnetic properties can be understood using...... the crystal-field parameters derived from the magnetic exciton spectrum measured by Houmann et al. by means of inelastic neutron scattering. The combined lambda -Schottky anomaly in the heat capacity is explained. No additional parameters are introduced....

  5. Vibrations on Al surfaces covered by sodium

    Science.gov (United States)

    Rusina, G. G.; Eremeev, S. V.; Borisova, S. D.; Sklyadneva, I. Yu.; Chulkov, E. V.

    2006-09-01

    In this paper we present the results of a comparative study of vibrational and structural properties of the ordered (√{3}×√{3})R30∘ and c(2 × 2) phases formed by Na adatoms at room temperature on the Al(1 1 1) and Al(1 0 0) surfaces, respectively. The surface relaxation, surface phonon dispersion, and polarization of vibrational modes are calculated using the embedded-atom method. Our calculated structural parameters are in agreement with experimental and ab initio results. The obtained vibrational frequencies compare fairly well with available experimental data.

  6. Metamagnetism in Ce(Ga,Al)2

    Indian Academy of Sciences (India)

    K G Suresh; S Radha; A K Nigam

    2002-05-01

    Effect of Al substitution on the magnetic properties of Ce(Ga1-Al)2 ( = 0, 0.1 and 0.5) system has been studied. The magnetic state of CeGa2 is found to be FM with a C of 8 K, whereas the compounds with =0.1 and 0.5 are AFM and possess N of about 9 K. These two compounds undergo metamagnetic transition and the critical fields are about 1.2 T and 0.5 T, respectively at 2 K. These variations are explained on the basis of helical spin structure in these compounds.

  7. Instability of TiC and TiAl3 compounds in Al-10Mg and Al-5Cu alloys by addition of Al-Ti-C master alloy

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    The performance of Al-Ti-C master alloy in refining Al-10Mg and A1-5Cu alloys was studied by using electron probe micro-analyzer (EPMA) and X-ray diffractometer (XRD) analysis.The results indicate that there are obvious fading phenomena in both Al-10Mg and Al-5Cu alloys with the addition of Al-5Ti-0.4C refiner which contains TiC and TiAl3 compounds.Mg element has no influence on the stability of TiC and TiAl3, while TiC particles in Al-10Mg alloy react with Al to form Al4C3 particles, resulting in the refinement fading.However, TiC particles are relatively stable in Al-5Cu alloy, while TiAl3 phase reacts with Al2Cu to produce a new phase Ti(Al, Cu)2, which is responsible for the refinement fading in Al-5Cu alloy.These indicate that the refinement fading will not occur only when both the TiC particles and TiAl3 compound of Al-Ti-C refiner are stable in Al alloys.

  8. Directional Solidification and Mechanical Properties of NiAl-NiAlTa Alloys

    Science.gov (United States)

    Johnson, D. R.; Chen, X. F.; Oliver, B. F.; Noebe, R. D.; Whittenberger, J. D.

    1995-01-01

    Directional solidification of eutectic alloys is a promising technique for producing in-situ composite materials exhibiting a balance of properties. Consequently, the microstructure, creep strength and fracture toughness of directionally solidified NiAl-NiAlTa alloys were investigated. Directional solidification was performed by containerless processing techniques to minimize alloy contamination. The eutectic composition was found to be NiAl-15.5 at% Ta and well-aligned microstructures were produced at this composition. A near-eutectic alloy of NiAl-14.5Ta was also investigated. Directional solidification of the near-eutectic composition resulted in microstructures consisting of NiAl dendrites surrounded by aligned eutectic regions. The off-eutectic alloy exhibited promising compressive creep strengths compared to other NiAl-based intermetallics, while preliminary testing indicated that the eutectic alloy was competitive with Ni-base single crystal superalloys. The room temperature toughness of these two-phase alloys was similar to that of polycrystalline NiAl even with the presence of the brittle Laves phase NiAlTa.

  9. Electronic states and spin-forbidden cooling transitions of AlH and AlF.

    Science.gov (United States)

    Wells, Nathan; Lane, Ian C

    2011-11-14

    The feasibility of laser cooling AlH and AlF is investigated using ab initio quantum chemistry. All the electronic states corresponding to the ground and lowest two excited states of the Al atom are calculated using multi-reference configuration interaction (MRCI) and the large AV6Z basis set for AlH. The smaller AVQZ basis set is used to calculate the valence electronic states of AlF. Theoretical Franck-Condon factors are determined for the A(1)Π→ X(1)Σ(+) transitions in both radicals and found to agree with the highly diagonal factors found experimentally, suggesting computational chemistry is an effective method for screening suitable laser cooling candidates. AlH does not appear to have a transition quite as diagonal as that in SrF (which has been laser cooled) but the A(1)Π→ X(1)Σ(+) transition transition of AlF is a strong candidate for cooling with just a single laser, though the cooling frequency is deep in the UV. Furthermore, the a(3)Π→ X(1)Σ(+) transitions are also strongly diagonal and in AlF is a practical method for obtaining very low final temperatures around 3 μK.

  10. Electronic circuits having NiAl and Ni.sub.3 Al substrates

    Energy Technology Data Exchange (ETDEWEB)

    Deevi, Seetharama C. (Midlothian, VA); Sikka, Vinod K. (Oak Ridge, TN)

    1999-01-01

    An electronic circuit component having improved mechanical properties and thermal conductivity comprises NiAl and/or Ni.sub.3 Al, upon which an alumina layer is formed prior to applying the conductive elements. Additional layers of copper-aluminum alloy or copper further improve mechanical strength and thermal conductivity.

  11. Electronic circuits having NiAl and Ni{sub 3}Al substrates

    Energy Technology Data Exchange (ETDEWEB)

    Deevi, S.C.; Sikka, V.K.

    1999-10-12

    An electronic circuit component having improved mechanical properties and thermal conductivity comprises NiAl and/or Ni{sub 3}Al, upon which an alumina layer is formed prior to applying the conductive elements. Additional layers of copper-aluminum alloy or copper further improve mechanical strength and thermal conductivity.

  12. Muslih b. `Abd al-Rahman al-Maraqî : Muslikh of Mranggen

    NARCIS (Netherlands)

    Bruinessen, M.M. van

    2007-01-01

    MUSLIH b. `ABD AL-RAHMAN AL-MARAQÎ (KH. Muslikh of Mranggen) [Indonesia; 1917-1981] [Dictionnaire biographique des savants et grandes figures du monde musulman périphérique, du XIXe siècle à nos jours, Fasc. no 2. Paris: CNRS-EHESS, 1998, p. 23-24] One of the most influential Javanese teachers of t

  13. Fabrication and Characterization of AL-AL4C3 Nanocomposite by Mechanical Alloying

    Science.gov (United States)

    Mohammad Sharifi, E.; Enayati, M. H.; Karimzadeh, F.

    Aluminum carbide (Al4C3) seems to be an ideal reinforcement for producing aluminum matrix composites. Al4C3 has high hardness and shear strength as well as a high melting point. The dispersion of Al4C3 particles in the matrix produces a pinning effect that reduces aluminum grain growth, which improves the mechanical properties. In this study, aluminum powders were mixed with 4.5 wt.% graphite and mechanically alloyed using a high-energy ball mill in order to produce Al-Al4C3 nanocomposite. The structural evaluation of powder particles after different milling times was studied by X-ray diffractometry, scanning electron microscopy and microhardness measurements. The aluminum crystallite size estimated with broadening of XRD peaks by Williamson-Hall formula. XRD results suggested that the grain size of aluminum decreased to nanometer range (30 nm) during ball milling. No Al4C3 formed during the mechanical alloying process. Milled powders were then annealed at 300-600 °C for 1 h under argon atmosphere. Annealing at temperatures higher than 300 °C led to formation of Al4C3 phase which increased as annealing temperature increased. Aluminum grain size remained in nanometer range after annealing process. The microhardness of powder particles increased after annealing. Both effects are due to the formation of nanosized Al4C3 particles.

  14. Sintering Behavior of CNT Reinforced Al6061 and Al2124 Nanocomposites

    Directory of Open Access Journals (Sweden)

    Nouari Saheb

    2014-01-01

    Full Text Available Ball milling and spark plasma sintering were successfully used to produce carbon nanotube reinforced Al6061 and Al2124 nanocomposites which have potential applications in the fields of aerospace, automotive, electronics, and high precision instrumentation. Al2124 and Al6061 nanocomposite powders containing 0.5 to 2 wt.% CNTs prepared through sonication and wet ball milling were spark plasma sintered at 400, 450, and 500°C for 20 minutes under a pressure of 35 MPa. CNTs were better dispersed, and less agglomerated and had good adhesion to the matrix in composites containing 1 wt.% CNTs. The increase of CNT content to 2 wt.% led to the formation of CNT clusters which resulted in less uniform and homogenous composite powders. Almost full densification of Al6061 reinforced with CNTs was achieved at 500°C. Also, CNTs reinforced Al2124 nanocomposites reached very high densities at 500°C. Composites reinforced with 1 wt.% CNTs displayed better densification compared to composites containing 2 wt.% CNTs. The increase of CNTs content from 0.5 to 1 wt.% increased the hardness of the Al6061 and Al2124 alloys to maximum values. Further increase of CNTs content to 2 wt.% decreased the hardness to values lower than that of the monolithic alloys.

  15. Thermodynamic Assessments of Al-In-Sb and Al-In-As Systems

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    The experimental thermodynamic and phase diagram data of the Al-In-Sb and Al-In-As ternary systems were critically assessed.A set of self-consistent thermodynamic model parameters were obtained to describe the phase equilibria and the thermodynamic properties of two systems.In most cases,the calculated values agree very well with the experimental data.

  16. Synthesis of TiAl-Ti2AlN composites by in-situ crystallization

    Institute of Scientific and Technical Information of China (English)

    ZHU Jian-feng; GAO Ji-qiang; WANG Fen

    2006-01-01

    The amorphization process during mechanical alloying (MA) was investigated for the Ti-50%/Al (mole fraction) powder mixtures with no special protection conditions. During the milling process, with the milling time prolonging, the metallic powder Ti and Al were finely mixed, gradually, aluminum completely dissolved into titanium to form an Ti(Al) hcp supersaturated solid solution, and finally, transformed to the amorphous phase after milled for about 39 h. As a result of heat treatment in hot press sintering processing for the mechanically alloyed amorphous powders in vacuum, a submicrostructure intermetallics of TiAl/Ti2AlN composite can be produced by in-situ crystallization. Furthermore, the structure evolution, phase formation and transformation during the heat treatment were also investigated by X-ray diffractometry and differential thermal analysis. The results show that the reaction involves many transitional stages, including formation of TiAl3 and transformation into TiAl and Ti3Al. The examination show that the composite materials fabricated by this in-situ crystallization from amorphization have good mechanical properties due to fine grain size and uniform microstructure.

  17. MgAl2O4–-Al2O3 solid solution interaction: mathematical framework and phase separation of -Al2O3 at high temperature

    Indian Academy of Sciences (India)

    Soumen Pal; A K Bandyopadhyay; S Mukherjee; B N Samaddar; P G Pal

    2011-07-01

    Although existence of MgAl2O4–-Al2O3 solid solution has been reported in the past, the detailed interactions have not been explored completely. For the first time, we report here a mathematical framework for the detailed solid solution interactions of -Al2O3 in MgAl2O4 (spinel). To investigate the solid solubility of -Al2O3 in MgAl2O4, Mg–Al spinel (MgO–Al2O3; = 1, 1.5, 3, 4.5 and an arbitrary high value 30) precursors have been heat treated at 1000°C. Presence of only non-stoichiometric MgAl2O4 phase up to = 4.5 at 1000°C indicates that alumina (as -Al2O3) present beyond stoichiometry gets completely accommodated in MgAl2O4 in the form of solid solution. → alumina phase transformation and its subsequent separation from MgAl2O4 has been observed in the Mg–Al spinel powders ( > 1) when the 1000°C heat treated materials are calcined at 1200°C. In the mathematical framework, unit cell of MgAl2O4 (Mg8Al16O32) has been considered for the solid solution interactions (substitution of Mg2+ ions by Al3+ ions) with -Al2O3. It is suggested that combination of unit cells of MgAl2O4 takes part in the interactions when > 5 (MgO–Al2O3).

  18. FABRICATION OF Al/Al2O3 FGM ROTATING DISC

    Directory of Open Access Journals (Sweden)

    A. B. Sanuddin

    2012-06-01

    Full Text Available This study presents a method of fabricating a disc made of Al/Al2O3 functionally graded materials (FGM, using a powder metallurgy manufacturing process. The aim is to develop a processing method for a rotating disc made of FGM, by stacking the slurry, layer by layer in a radial direction. A three-layer functionally graded material of Al/Al2O3 is fabricated with compositions of 10, 20, 30 vol.% Al2O3. The ceramic composition increases from the discs inner (centre to the outer. The combination of these materials can offer the ability to withstand high temperature conditions whilst maintaining strength in extreme environments.

  19. The anodic and cathodic dissolution of Al and Al-Cu-Mg alloy

    Energy Technology Data Exchange (ETDEWEB)

    Mokaddem, M.; Volovitch, P. [Laboratoire de physicochimie des surfaces, UMR7045, Ecole Nationale Superieure de Chimie de Paris, ParisTech, 11, rue Pierre et Marie Curie, Paris (France); Rechou, F.; Oltra, R. [Institut CARNOT de Bourgogne, Electrochimie Interfaciale-Corrosion, UMR5209, Universite de Bourgogne, Dijon (France); Ogle, K., E-mail: kevin-ogle@enscp.f [Laboratoire de physicochimie des surfaces, UMR7045, Ecole Nationale Superieure de Chimie de Paris, ParisTech, 11, rue Pierre et Marie Curie, Paris (France)

    2010-04-15

    Atomic emission spectroelectrochemistry (AESEC) was used to monitor the release of Al from 99.99% aluminum (1199 alloy) and Al, Mg, and Cu from 2024 Al alloy in 30 g/l NaCl electrolyte as a function of pH. The cathodic dissolution of Al was demonstrated and attributed to an increase in the pH at the interface due to the water reduction reaction. The dissolution of Mg was also observed but was a more complex function of current probably depending on the interfacial pH and the Al dissolution rate. The detachment of copper-rich particles was observed as very rapid spectroscopic emission transients (peak width < 10 ms).

  20. Hydrogenation properties of Mg-Al alloys

    DEFF Research Database (Denmark)

    Andreasen, Anders

    2008-01-01

    to disproportionation with the formation of magnesium hydride and metallic aluminum as the final product. Experimental evidence renders this process reversible. It is observed that the enthalpy of hydride formation of magnesium is lowered upon alloying with Al due to a slightly endothermic disproportionation reaction...