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Sample records for analysis tie-hua ng

  1. Analysis of Coexisting GPON and NG-PON1 (10G-PON Systems

    Directory of Open Access Journals (Sweden)

    M. D. Mraković

    2011-06-01

    Full Text Available In this paper, the simulation model of coexisting GPON and NG-PON1 (10G-PON systems is presented, which has been developed for the analysis of feasibility and implementation issues of this coexistence. The aim was to analyze the impact of the most important parameters of the components that are needed for new network elements, on the performance of these coexistent networks. On the basis of the results obtained, the optimal parameters of the new system components were defined.

  2. NG09 And CTBT On-Site Inspection Noble Gas Sampling and Analysis Requirements

    Science.gov (United States)

    Carrigan, Charles R.; Tanaka, Junichi

    2010-05-01

    A provision of the Comprehensive Test Ban Treaty (CTBT) allows on-site inspections (OSIs) of suspect nuclear sites to determine if the occurrence of a detected event is nuclear in origin. For an underground nuclear explosion (UNE), the potential success of an OSI depends significantly on the containment scenario of the alleged event as well as the application of air and soil-gas radionuclide sampling techniques in a manner that takes into account both the suspect site geology and the gas transport physics. UNE scenarios may be broadly divided into categories involving the level of containment. The simplest to detect is a UNE that vents a significant portion of its radionuclide inventory and is readily detectable at distance by the International Monitoring System (IMS). The most well contained subsurface events will only be detectable during an OSI. In such cases, 37 Ar and radioactive xenon cavity gases may reach the surface through either "micro-seepage" or the barometric pumping process and only the careful siting of sampling locations, timing of sampling and application of the most site-appropriate atmospheric and soil-gas capturing methods will result in a confirmatory signal. The OSI noble gas field tests NG09 was recently held in Stupava, Slovakia to consider, in addition to other field sampling and analysis techniques, drilling and subsurface noble gas extraction methods that might be applied during an OSI. One of the experiments focused on challenges to soil-gas sampling near the soil-atmosphere interface. During withdrawal of soil gas from shallow, subsurface sample points, atmospheric dilution of the sample and the potential for introduction of unwanted atmospheric gases were considered. Tests were designed to evaluate surface infiltration and the ability of inflatable well-packers to seal out atmospheric gases during sample acquisition. We discuss these tests along with some model-based predictions regarding infiltration under different near

  3. Induction Effect and Genetic Analysis of NG to Thallus of Porphyra

    Institute of Scientific and Technical Information of China (English)

    Xu Pu; Shen Songdong; Fei Xiugeng; Zhang Xuecheng; Zhu Jianyi

    2002-01-01

    The young thalli of Porphyra yezoensis and P. haitanensis are induced by N-Methy-N'-Nitro-N-Nitrosoguanidine (NG), a strong mutagen to induce the mutant of chromatophore. It is showed that no mutants have been investigated in all control groups,while in the induced groups, it is easy to find mutant cells or mutant cell masses in the thallus.The pigmentation mutants may be the result of NG inducement. The mutant rate increases obviously with the mutagen concentration or by prolonging the induction time. Within the scope of experiment, the inducement effects of Porphyra yezoensis are raised from 11.2% to 28.7%, and those of P. haitanensis from 10.1% to 20.2%. According to our experiments, the amount of mutant cells in the same area does not increase with the induction intensity The amount of mutant cells in every group of inducement of both species of Porphyra changes very little with the fixed fields check method. The lowest dose (25μg/mL) of this experiment has a high mutant rate. The mutant rate shows the percentage of mutant cells in all the survived cells, while the amount of mutant cells reflects the effect of inducement. Thus the optimum induction concentration of NG is 25μg/mL, and the optimum induction time is 30minutes.

  4. Analysis of axonal regeneration in the central and peripheral nervous systems of the NG2-deficient mouse

    Directory of Open Access Journals (Sweden)

    Lieberman Alexander R

    2007-09-01

    Full Text Available Abstract Background The chondroitin sulphate proteoglycan NG2 blocks neurite outgrowth in vitro and has been proposed as a major inhibitor of axonal regeneration in the CNS. Although a substantial body of evidence underpins this hypothesis, it is challenged by recent findings including strong expression of NG2 in regenerating peripheral nerve. Results We studied axonal regeneration in the PNS and CNS of genetically engineered mice that do not express NG2, and in sex and age matched wild-type controls. In the CNS, we used anterograde tracing with BDA to study corticospinal tract (CST axons after spinal cord injury and transganglionic labelling with CT-HRP to trace ascending sensory dorsal column (DC axons after DC lesions and a conditioning lesion of the sciatic nerve. Injury to these fibre tracts resulted in no difference between knockout and wild-type mice in the ability of CST axons or DC axons to enter or cross the lesion site. Similarly, after dorsal root injury (with conditioning lesion, most regenerating dorsal root axons failed to grow across the dorsal root entry zone in both transgenic and wild-type mice. Following sciatic nerve injuries, functional recovery was assessed by analysis of the toe-spreading reflex and cutaneous sensitivity to Von Frey hairs. Anatomical correlates of regeneration were assessed by: retrograde labelling of regenerating dorsal root ganglion (DRG cells with DiAsp; immunostaining with PGP 9.5 to visualise sensory reinnervation of plantar hindpaws; electron microscopic analysis of regenerating axons in tibial and digital nerves; and by silver-cholinesterase histochemical study of motor end plate reinnervation. We also examined functional and anatomical correlates of regeneration after injury of the facial nerve by assessing the time taken for whisker movements and corneal reflexes to recover and by retrograde labelling of regenerated axons with Fluorogold and DiAsp. None of the anatomical or functional analyses

  5. Intramuscular depot formulations of leuprolide acetate suppress testosterone levels below a 20 ng/dL threshold: a retrospective analysis of two Phase III studies

    Directory of Open Access Journals (Sweden)

    Spitz A

    2016-08-01

    Full Text Available Aaron Spitz,1 Marc Gittelman,2 Lawrence I Karsh,3 Sanja Dragnic,4 Ahmed M Soliman,5 Aditya Lele,6 Damian Gruca,7 Michael Norton4 1Orange County Urology Associates, Laguna Beach, CA, 221st Century Oncology/UroMedix-Aventura Division, Aventura, FL, 3The Urology Center of Colorado, Denver, CO, 4US Medical Affairs, 5Health Economics and Outcomes Research, 6Data and Statistical Sciences, AbbVie Inc., North Chicago, IL, USA; 7Global Medical Affairs, AbbVie Deutschland, Ludwigshafen, Germany Introduction: Androgen deprivation therapy (ADT with gonadotropin-releasing hormone (GnRH analogs is a standard treatment for advanced prostate cancer. GnRH analog therapy can reduce testosterone to “castrate” levels, historically defined as <50 ng/dL. With the advent of newer assays, a lower threshold of <20 ng/dL has recently been proposed. We report the results of a retrospective analysis of two Phase III trials of 4- and 6-month depot microsphere formulations of leuprolide acetate (LA, a GnRH agonist that has previously demonstrated efficacy in testosterone suppression to <50 ng/dL in patients on ADT. This analysis investigates the ability of these LA formulations to suppress to ≤20 ng/dL levels.Methods: In two of five AbbVie/Abbott clinical trials of microsphere formulations of LA for ADT, analytic technology permitting testosterone detection as low as 3 ng/dL was used and thus was selected for this analysis. Both trials were open-label, fixed-dose studies in prostate cancer patients, naïve to ADT. Patients received either 30 mg (4-month formulation; n=49 or 45 mg (6-month formulation; n=151 depot injections of LA microspheres. Treatment duration was up to 32 weeks for the 4-month formulation and 48 weeks for the 6-month formulation. The proportion of patients achieving the 20 ng/dL threshold was determined every 4 weeks.Results: Pooled analysis showed that 152 of 193 (79% of patients achieved serum testosterone levels of ≤20 ng/dL at 4 weeks, and

  6. Hagkis ng Halakhak: Ang Nagpapatuloy na Kabuluhan ng Kritika ni Hernandez sa Pulitika sa Magkabilang Mukha ng Isang Bagol

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    Rosario Torres-Yu

    2012-12-01

    Full Text Available Itinanong ng kritikong si Epifanio San Juan, Jr., sa okasyon ng paggunita sa sentenaryo ng pagsilang ni Amado V. Hernandez, kung bakit wala pang panunuring historikal, liban sa isinulat ni Agoncillo at Lumbera, sa mga kuwento, dula, at sanaysay ng Pambansang Alagad sa Panitikan. Isang tugon ang panunuring ito sa isang dula ni Hernandez. Susuriin ang kahalagahang panlipunan at pangkasaysayan ng dula niya:1 sa lente mismo ng dalumat ni Hernandez sa relasyon ng panitikan at ng lipunan, 2 sa konteksto ng tagisang ideolohikal ng dula niya at ng panahong nasulat ang dula, at3 sa pangkasalukuyang sipat sa mga ito bilang diskurso na may diin sa usapin ng kapangyarihan at “katotohanan.” Malapitang susuriin, lalo na ang may kaugnayan sapangatlong pagbasa, ang dulang Magkabilang Mukha ng Isang Bagol (1961, dahil sa palagay ng nagsusuri na sa dulang ito higit na makikita na nagpapatuloy angkabuluhan ni Hernandez sa kasalukuyan, at dito lumulutang ang lakas at kapangyarihan ng kanyang kritika sa sistemang pampulitika.Writer and critic Epifanio San Juan Jr. observed, on the occasion of the centenary of the birth of Amado V. Hernandez, that except for the works of Agoncillo and Lumbera, no other historical analysis, particularly of the essays and plays, of the National Artist Hernandez has been written. This paper is an attempt to fill the gap. It evaluates the social and historical significance of one of his plays, Magkabilang Mukha ng Isang Bagol (1961, through the lens of Hernandez’s view of the relations of literature and society and in the context of the ideological position of his play and the ideological struggle atthe time the play was written. The play is treated, too, as discourse and centers on the discussion of power and “truth.” It is this writer’s position that it is in this play that Hernandez’s continuing relevance to the present ideological debaterests. It is also in this play that the strength of his critique is clearly

  7. TestNG beginner's guide

    CERN Document Server

    Menon, Varun

    2013-01-01

    This book is written in a friendly, beginner's guide style with plenty of step-by-step instructions with appropriate examples.This book is great for developers and testers who are new to TestNg and want to learn how to use TestNG for writing their application as well as functional tests. This book assumes that you have experience in Java and OOPs concepts and have worked with certain IDE.

  8. Analysis of slow-onset neurite formation in NG108-15 cells: implications for a unified model of neurite elongation.

    Science.gov (United States)

    Smalheiser, N R

    1989-01-01

    When undifferentiated NG108-15 cells are plated onto polylysine coated Petri dishes in serum-free medium, they form neurites within 1-4 h if plated in the presence of laminin or 5'-deoxy-5'-methylthioadenosine (rapid-onset neurites). In the absence of such agents, serum-deprived NG108-15 cells extend axon-like neurites onto polylysine over several days; here we characterize the dynamic behavior of this slow-onset outgrowth pattern in detail. Individual cells plated on laminin expressed a gradual multipolar-to-unipolar transition due to rapid-onset neurites becoming remodelled into the appearance of slow-onset neurites. This phenomenon reflected the selective stabilization of certain rapid-onset neurites, along with the restriction of motility to their distal tips. Based upon the properties and interactions of both rapid- and slow-onset neurites in NG108-15 cells, a unified model for neurite formation is presented. PMID:2917412

  9. Serum 25-Hydroxyvitamin D Concentrations ≥40 ng/ml Are Associated with >65% Lower Cancer Risk: Pooled Analysis of Randomized Trial and Prospective Cohort Study.

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    Sharon L McDonnell

    Full Text Available Higher serum 25-hydroxyvitamin D [25(OHD] concentrations have been associated with a lower risk of multiple cancer types across a range of 25(OHD concentrations.To investigate whether the previously reported inverse association between 25(OHD and cancer risk could be replicated, and if a 25(OHD response region could be identified among women aged 55 years and older across a broad range of 25(OHD concentrations.Data from two cohorts representing different median 25(OHD concentrations were pooled to afford a broader range of 25(OHD concentrations than either cohort alone: the Lappe cohort (N = 1,169, a randomized clinical trial cohort (median 25(OHD = 30 ng/ml and the GrassrootsHealth cohort (N = 1,135, a prospective cohort (median 25(OHD = 48 ng/ml. Cancer incidence over a multi-year period (median: 3.9 years was compared according to 25(OHD concentration. Kaplan-Meier plots were developed and the association between 25(OHD and cancer risk was examined with multivariate Cox regression using multiple 25(OHD measurements and spline functions. The study included all invasive cancers excluding skin cancer.Age-adjusted cancer incidence across the combined cohort (N = 2,304 was 840 cases per 100,000 person-years (1,020 per 100,000 person-years in the Lappe cohort and 722 per 100,000 person-years in the GrassrootsHealth cohort. Incidence was lower at higher concentrations of 25(OHD. Women with 25(OHD concentrations ≥40 ng/ml had a 67% lower risk of cancer than women with concentrations <20 ng/ml (HR = 0.33, 95% CI = 0.12-0.90.25(OHD concentrations ≥40 ng/ml were associated with substantial reduction in risk of all invasive cancers combined.

  10. Serum 25-Hydroxyvitamin D Concentrations ≥40 ng/ml Are Associated with >65% Lower Cancer Risk: Pooled Analysis of Randomized Trial and Prospective Cohort Study

    Science.gov (United States)

    McDonnell, Sharon L.; Baggerly, Carole; French, Christine B.; Baggerly, Leo L.; Garland, Cedric F.; Gorham, Edward D.; Lappe, Joan M.; Heaney, Robert P.

    2016-01-01

    Background Higher serum 25-hydroxyvitamin D [25(OH)D] concentrations have been associated with a lower risk of multiple cancer types across a range of 25(OH)D concentrations. Objectives To investigate whether the previously reported inverse association between 25(OH)D and cancer risk could be replicated, and if a 25(OH)D response region could be identified among women aged 55 years and older across a broad range of 25(OH)D concentrations. Methods Data from two cohorts representing different median 25(OH)D concentrations were pooled to afford a broader range of 25(OH)D concentrations than either cohort alone: the Lappe cohort (N = 1,169), a randomized clinical trial cohort (median 25(OH)D = 30 ng/ml) and the GrassrootsHealth cohort (N = 1,135), a prospective cohort (median 25(OH)D = 48 ng/ml). Cancer incidence over a multi-year period (median: 3.9 years) was compared according to 25(OH)D concentration. Kaplan-Meier plots were developed and the association between 25(OH)D and cancer risk was examined with multivariate Cox regression using multiple 25(OH)D measurements and spline functions. The study included all invasive cancers excluding skin cancer. Results Age-adjusted cancer incidence across the combined cohort (N = 2,304) was 840 cases per 100,000 person-years (1,020 per 100,000 person-years in the Lappe cohort and 722 per 100,000 person-years in the GrassrootsHealth cohort). Incidence was lower at higher concentrations of 25(OH)D. Women with 25(OH)D concentrations ≥40 ng/ml had a 67% lower risk of cancer than women with concentrations <20 ng/ml (HR = 0.33, 95% CI = 0.12–0.90). Conclusions 25(OH)D concentrations ≥40 ng/ml were associated with substantial reduction in risk of all invasive cancers combined. PMID:27049526

  11. Experimental and theoretical studies of the infrared spectra and bonding properties of NgBeCO₃ and a comparison with NgBeO (Ng = He, Ne, Ar, Kr, Xe).

    Science.gov (United States)

    Zhang, Qingnan; Chen, Mohua; Zhou, Mingfei; Andrada, Diego M; Frenking, Gernot

    2015-03-19

    The novel neon complex NeBeCO3 has been prepared in a low-temperature neon matrix via codeposition of laser-evaporated beryllium atoms with O2 + CO/Ne. Doping by the heavier noble gas atoms argon, krypton and xenon yielded the associated adducts NgBeCO3 (Ng = Ar, Kr, Xe). The noble gas complexes have been identified via infrared spectroscopy. Quantum chemical calculations of NgBeCO3 and NgBeO (Ng = He, Ne, Ar, Kr, Xe) using ab initio methods and density functional theory show that the Ng-BeCO3 bonds are slightly longer and weaker than the Ng-BeO bonds. The energy decomposition analysis of the Ng-Be bonds suggests that the attractive interactions come mainly from the Ng → BeCO3 and Ng → BeO σ donation.

  12. Analysis on ATC Problems of Boeing 737NG Airplane%波音737NG飞机ATC FAIL故障浅析

    Institute of Scientific and Technical Information of China (English)

    罗康

    2015-01-01

    The occurrence frequency of ATC problems of Boeing 737NG Airplane is high in the running, as it cooperated with TCAS, it has great obstacles of the airplane’s normal operation and flight delay. This paper will analyze the fail of ACT.%波音737NG飞机在运行中经常会出现ATC FAIL故障,由于该系统牵涉到飞机的防撞系统,对飞机的正常运行产生很大的影响,引起航班延误,本文对该故障现象进行浅析。

  13. Exploring the Nature of Silicon-Noble Gas Bonds in H3SiNgNSi and HSiNgNSi Compounds (Ng = Xe, Rn

    Directory of Open Access Journals (Sweden)

    Sudip Pan

    2015-03-01

    Full Text Available Ab initio and density functional theory-based computations are performed to investigate the structure and stability of H3SiNgNSi and HSiNgNSi compounds (Ng = Xe, Rn. They are thermochemically unstable with respect to the dissociation channel producing Ng and H3SiNSi or HSiNSi. However, they are kinetically stable with respect to this dissociation channel having activation free energy barriers of 19.3 and 23.3 kcal/mol for H3SiXeNSi and H3SiRnNSi, respectively, and 9.2 and 12.8 kcal/mol for HSiXeNSi and HSiRnNSi, respectively. The rest of the possible dissociation channels are endergonic in nature at room temperature for Rn analogues. However, one three-body dissociation channel for H3SiXeNSi and one two-body and one three-body dissociation channels for HSiXeNSi are slightly exergonic in nature at room temperature. They become endergonic at slightly lower temperature. The nature of bonding between Ng and Si/N is analyzed by natural bond order, electron density and energy decomposition analyses. Natural population analysis indicates that they could be best represented as (H3SiNg+(NSi− and (HSiNg+(NSi−. Energy decomposition analysis further reveals that the contribution from the orbital term (ΔEorb is dominant (ca. 67%–75% towards the total attraction energy associated with the Si-Ng bond, whereas the electrostatic term (ΔEelstat contributes the maximum (ca. 66%–68% for the same in the Ng–N bond, implying the covalent nature of the former bond and the ionic nature of the latter.

  14. The NG2 Protein Is Not Required for Glutamatergic Neuron-NG2 Cell Synaptic Signaling.

    Science.gov (United States)

    Passlick, Stefan; Trotter, Jacqueline; Seifert, Gerald; Steinhäuser, Christian; Jabs, Ronald

    2016-01-01

    NG2 glial cells (as from now NG2 cells) are unique in receiving synaptic input from neurons. However, the components regulating formation and maintenance of these neuron-glia synapses remain elusive. The transmembrane protein NG2 has been considered a potential mediator of synapse formation and alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR) clustering, because it contains 2 extracellular Laminin G/Neurexin/Sex Hormone-Binding Globulin domains, which in neurons are crucial for formation of transsynaptic neuroligin-neurexin complexes. NG2 is connected via Glutamate Receptor-Interacting Protein with GluA2/3-containing AMPARs, thereby possibly mediating receptor clustering in glial postsynaptic density. To elucidate the role of NG2 in neuron-glia communication, we investigated glutamatergic synaptic transmission in juvenile and aged hippocampal NG2 cells of heterozygous and homozygous NG2 knockout mice. Neuron-NG2 cell synapses readily formed in the absence of NG2. Short-term plasticity, synaptic connectivity, postsynaptic AMPAR current kinetics, and density were not affected by NG2 deletion. During development, an NG2-independent acceleration of AMPAR current kinetics and decreased synaptic connectivity were observed. Our results indicate that the lack of NG2 does not interfere with genesis and basic properties of neuron-glia synapses. In addition, we demonstrate frequent expression of neuroligins 1-3 in juvenile and aged NG2 cells, suggesting a role of these molecules in synapse formation between NG2 glia and neurons.

  15. Ang proseso ng transkripsiyon ng mga inskripsiyon sa mga Bato ng Ticao

    Directory of Open Access Journals (Sweden)

    Ramon Guillermo

    2012-06-01

    Full Text Available The so-called ‘Ticao Stones’ that were announced to the public last 2011 were the first stone inscriptions in the baybayin script to have been discovered in the Philippines. This article is a report of the transcription of the inscription which was collectively produced by the interdisciplinary University of the Philippines (U.P. Ticao, Masbate Anthropological Project Team. This report includes a systematic analysis of the inscriptions and some suggestions towards a possible reading of the inscriptions. Some issues relevant to the problem of the authenticity of the stones also raised.

  16. Neuron-NG2 Cell Synapses: Novel Functions for Regulating NG2 Cell Proliferation and Differentiation

    Directory of Open Access Journals (Sweden)

    Qian-Kun Yang

    2013-01-01

    Full Text Available NG2 cells are a population of CNS cells that are distinct from neurons, mature oligodendrocytes, astrocytes, and microglia. These cells can be identified by their NG2 proteoglycan expression. NG2 cells have a highly branched morphology, with abundant processes radiating from the cell body, and express a complex set of voltage-gated channels, AMPA/kainate, and GABA receptors. Neurons notably form classical and nonclassical synapses with NG2 cells, which have varied characteristics and functions. Neuron-NG2 cell synapses could fine-tune NG2 cell activities, including the NG2 cell cycle, differentiation, migration, and myelination, and may be a novel potential therapeutic target for NG2 cell-related diseases, such as hypoxia-ischemia injury and periventricular leukomalacia. Furthermore, neuron-NG2 cell synapses may be correlated with the plasticity of CNS in adulthood with the synaptic contacts passing onto their progenies during proliferation, and synaptic contacts decrease rapidly upon NG2 cell differentiation. In this review, we highlight the characteristics of classical and nonclassical neuron-NG2 cell synapses, the potential functions, and the fate of synaptic contacts during proliferation and differentiation, with the emphasis on the regulation of the NG2 cell cycle by neuron-NG2 cell synapses and their potential underlying mechanisms.

  17. SAS - en flygande beslutsgång

    OpenAIRE

    Haglund, Erik; Hjerpe, Anders

    2004-01-01

    A1146 Problem: De frågor som ställs är: Vilken slags beslutsgång och vilka beslutstyper det studerade företaget SAS har valt? Den klassiska rationella beslutsmodellen har fått utstå en viss kritik, men kan företag tillämpa modellen ändå? Vilka fördelar respektive nackdelar finns det om ett företag väljer att använda sig av en rationell beslutsgång? Syfte: Syftet med denna uppsats är att beskriva och analysera SAS koncernens beslutsgång. Det som avses med SAS:s beslutsgång är den förändringspr...

  18. Bitcoin-NG: A Scalable Blockchain Protocol

    OpenAIRE

    Eyal, Ittay; Gencer, Adem Efe; Sirer, Emin Gun; van Renesse, Robbert

    2015-01-01

    Cryptocurrencies, based on and led by Bitcoin, have shown promise as infrastructure for pseudonymous online payments, cheap remittance, trustless digital asset exchange, and smart contracts. However, Bitcoin-derived blockchain protocols have inherent scalability limits that trade-off between throughput and latency and withhold the realization of this potential. This paper presents Bitcoin-NG, a new blockchain protocol designed to scale. Based on Bitcoin's blockchain protocol, Bitcoin-NG is By...

  19. PVT-NG sensor final report.

    Energy Technology Data Exchange (ETDEWEB)

    Mitchell, Dean James; Brusseau, Charles A.

    2012-01-01

    This document is a final report for the polyvinyl toluene (PVT) neutron-gamma (PVT-NG) project, which was sponsored by the Domestic Nuclear Detection Office (DNDO). The PVT-NG sensor uses PVT detectors for both gamma and neutron detection. The sensor exhibits excellent spectral resolution and gain stabilization, which are features that are beneficial for detection of both gamma-ray and neutron sources. In fact, the ability to perform isotope identification based on spectra that were measured by the PVT-NG sensor was demonstrated. As described in a previous report, the neutron sensitivity of the first version of the prototype was about 25% less than the DNDO requirement of 2.5 cps/ng for bare Cf-252. This document describes design modifications that were expected to improve the neutron sensitivity by about 50% relative to the PVT-NG prototype. However, the project was terminated before execution of the design modifications after portal vendors demonstrated other technologies that enable neutron detection without the use of He-3. Nevertheless, the PVT-NG sensor development demonstrated several performance goals that may be useful in future portal designs.

  20. The NG2 Protein Is Not Required for Glutamatergic Neuron-NG2 Cell Synaptic Signaling.

    Science.gov (United States)

    Passlick, Stefan; Trotter, Jacqueline; Seifert, Gerald; Steinhäuser, Christian; Jabs, Ronald

    2016-01-01

    NG2 glial cells (as from now NG2 cells) are unique in receiving synaptic input from neurons. However, the components regulating formation and maintenance of these neuron-glia synapses remain elusive. The transmembrane protein NG2 has been considered a potential mediator of synapse formation and alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR) clustering, because it contains 2 extracellular Laminin G/Neurexin/Sex Hormone-Binding Globulin domains, which in neurons are crucial for formation of transsynaptic neuroligin-neurexin complexes. NG2 is connected via Glutamate Receptor-Interacting Protein with GluA2/3-containing AMPARs, thereby possibly mediating receptor clustering in glial postsynaptic density. To elucidate the role of NG2 in neuron-glia communication, we investigated glutamatergic synaptic transmission in juvenile and aged hippocampal NG2 cells of heterozygous and homozygous NG2 knockout mice. Neuron-NG2 cell synapses readily formed in the absence of NG2. Short-term plasticity, synaptic connectivity, postsynaptic AMPAR current kinetics, and density were not affected by NG2 deletion. During development, an NG2-independent acceleration of AMPAR current kinetics and decreased synaptic connectivity were observed. Our results indicate that the lack of NG2 does not interfere with genesis and basic properties of neuron-glia synapses. In addition, we demonstrate frequent expression of neuroligins 1-3 in juvenile and aged NG2 cells, suggesting a role of these molecules in synapse formation between NG2 glia and neurons. PMID:25100858

  1. Ang Kasaysayan at Panitikan sa Pagbuo ng Bayan: Rebyu ng Nobelang The Feet of Juan Bacnang ni F. Sionil Jose

    OpenAIRE

    Moreal Nagarit Camba

    2013-01-01

    Mababasa sa The Feet of Juan Bacnang (2011), ang pinakabagong nobela ni F. (Francisco) Sionil Jose o Frankie sa kanyang mga kaibigan, ang mahigpit na ugnayan ng manunulat at ng kanyang bayan. Siya ang nagsisilbing zeitgeist ng maraming panahon; ang espiritu at boses ng nakaraan at kasalukuyan, isang manunulat na kumakatha ng mga akdang sumasalamin sa mayamang karanasan at kasaysayan ng kultura at lahing pinag-usbungan. Kung saan ang kanyang nobelang Juan Bacnang, katulad ng iba pa niyang naun...

  2. Test Report for NG Sensors GTX-1000.

    Energy Technology Data Exchange (ETDEWEB)

    Manginell, Ronald P.

    2015-02-01

    This report describes initial testing of the NG Sensor GTX-1000 natural gas monitoring system. This testing showed that the retention time, peak area stability and heating value repeatability of the GTX-1000 were promising for natural gas measurements in the field or at the well head. The repeatability can be less than 0.25% for LHV and HHV for the Airgas standard tested in this report, which is very promising for a first generation prototype. Ultimately this system should be capable of 0.1% repeatability in heating value at significant size and power reductions compared with competing systems.

  3. Ang Kasaysayan at Panitikan sa Pagbuo ng Bayan: Rebyu ng Nobelang The Feet of Juan Bacnang ni F. Sionil Jose

    Directory of Open Access Journals (Sweden)

    Moreal Nagarit Camba

    2013-06-01

    Full Text Available Mababasa sa The Feet of Juan Bacnang (2011, ang pinakabagong nobela ni F. (Francisco Sionil Jose o Frankie sa kanyang mga kaibigan, ang mahigpit na ugnayan ng manunulat at ng kanyang bayan. Siya ang nagsisilbing zeitgeist ng maraming panahon; ang espiritu at boses ng nakaraan at kasalukuyan, isang manunulat na kumakatha ng mga akdang sumasalamin sa mayamang karanasan at kasaysayan ng kultura at lahing pinag-usbungan. Kung saan ang kanyang nobelang Juan Bacnang, katulad ng iba pa niyang naunang mga nobela, bagamat likhang-isip, ay repleksyon ng buhay, pasakit, at aspirasyon ng manunulat at lipunang lumikha rito; mga tunguhing hindi nalalayo sa layunin ng mga ninuno nitong anyong panitikan, ang mga epiko at kuwentong bayan. Ibig sabihin, ang akda bilang Weltanschauung na ayon kay Terry Eagleton ay “partikular na paraan ng pagtingin sa mundo; na may kaugnayan sa gahum na nagtatakda kung paano tingnan ang mundo na maituturing na ‘pag-iisip ng lipunan’ o ideyolohiya ng panahon”1 (sinipi ni Geok-Lin Lim A Modern National Epic 74. Dagdag pa rito, ang kanyang (mga nobela, partikular ang Juan Bacnang, ay nagsisilbing “siwang sa mga kurtinang-papel” sa kasaysayan;2 mga tala ng karanasan at pang-araw-araw na buhay, na madalas hindi naisusulat ng mga tradisyunal na historyador. Ito (ang mga tala, ayon kay Pierre Macherey, ang mga puwang o pinipi na boses na naisantabi ng maraming akdang pangkasaysayan (sinipi ni Eagleton Marxism and Literary Criticism 34-35.

  4. The Role of NG2 Proteoglycan in Glioma

    Directory of Open Access Journals (Sweden)

    Sridevi Yadavilli

    2016-02-01

    Full Text Available Neuron glia antigen-2 ((NG2, also known as chondroitin sulphate proteoglycan 4, or melanoma-associated chondroitin sulfate proteoglycan is a type-1 membrane protein expressed by many central nervous system (CNS cells during development and differentiation and plays a critical role in proliferation and angiogenesis. ‘NG2’ often references either the protein itself or the highly proliferative and undifferentiated glial cells expressing high levels of NG2 protein. NG2 glia represent the fourth major type of neuroglia in the mammalian nervous system and are classified as oligodendrocyte progenitor cells by virtue of their committed oligodendrocyte generation in developing and adult brain. Here, we discuss NG2 glial cells as well as NG2 protein and its expression and role with regards to CNS neoplasms as well as its potential as a therapeutic target for treating childhood CNS cancers.

  5. Complexes of the noble gases with H3O+: a theoretical investigation on Ng(H3O+) (Ng = He-Xe).

    Science.gov (United States)

    Borocci, Stefano; Cecchi, Patrizio; Giordani, Maria; Grandinetti, Felice

    2015-01-01

    The geometries, harmonic vibrational frequencies, and binding energies (Bes) of the Ng(H(3)O(+)) complexes (Ng = He-Xe) were investigated at the coupled cluster level of theory, and their bonding situation was assayed by various methods of bonding analysis. The effects of Ng on H(3)O(+) progressively increase from He to Xe, and only He can be regarded as an essentially "innocent" ligand. The binding energies also increase in the same periodic order, and are by far dominated by the "noncovalent" ion-induced dipole interaction arising from the H(3)O(+)-induced polarization of Ng. For Ne, Ar, Kr, and Xe, this term has a larger contribution from the p orbital lying on the bond axis, and two smaller contributions from the p orbitals perpendicular to the bond axis. For the heaviest Ar(H(3)O(+)), Kr(H(3)O(+)), and Xe(H(3)O(+)), BE also has a "covalent" component, which is ascribed to the relatively-appreciable charge transfer from Ng to H(3)O(+). PMID:26307697

  6. NG2 expression predicts the metastasis formation in soft-tissue sarcoma patients.

    Science.gov (United States)

    Benassi, Maria Serena; Pazzaglia, Laura; Chiechi, Antonella; Alberghini, Marco; Conti, Amalia; Cattaruzza, Sabrina; Wassermann, Bruna; Picci, Piero; Perris, Roberto

    2009-01-01

    Enhanced expression levels of NG2 proteoglycan in presurgical original lesions of soft-tissue sarcoma (STS) patients defines with 55% probability the immediate (i.e., within 12 months postsurgery) risk in these individuals to develop postsurgical secondary lesions, independently of any other clinical trait. It, therefore, provides a molecular factor that alone prospects a particularly unfavorable clinical outcome in such patients. Evaluation of the timing of metastasis formation in patients with high and low levels of NG2 in their primitive lesions further stratified the patients in subsets with diverse lag phases in the occurrence of metastatic disease. In our cohort of high-grade STS cases, transcription of NG2 also showed a 81-fold amplification in metastatic lesions, when compared to primitive ones, and this gene overexpression was accompanied by an abundant but nonuniform in situ expression of its product. In a similar manner as seen in primitive lesions, patients with higher levels of metastatic NG2 encountered a significantly more dismal clinical course. Multivariate analysis asserted that in these individuals upregulation of NG2 represented an absolute independent prognostic parameter. Therefore, minimally invasive assessment of the transcription levels of the NG2 gene represents a parameter capable of predicting the arising of metastatic disease within a definite postsurgery time interval, and affords in adjunct in the definition of life expectance in STS patients.

  7. Etendatakse 10-aastase kirjamehe näitemäng

    Index Scriptorium Estoniae

    2003-01-01

    Tartu katoliku kooli 3. klassi poisi Mario Raitari näitemäng "Kristoph Silvester von Tenderi lugu", mis on esimene lugu sarjast "Mario Raitari kroonika". Näidendit etendab Linnupuu Lastepereteatri trupp

  8. 智能脊柱理疗设备摆动机构设计与分析%Design and analysis of swingi ng mechanism of the intelligent spine physiotherapy equipment

    Institute of Scientific and Technical Information of China (English)

    张凯; 何非; 李东波

    2015-01-01

    In order to design the swinging mechanism that meets the functional requirements of the intelligent spine physiotherapy equipment , it compares the structural characteristics of several typical swinging mechanism , designs the mechanism for ensuring the realization of the function .Based on SolidWorks it establishes the whole 3D model,designs that the maximum swing speed is 100 times/min, the maximum load of the intelligent spine physiotherapy equipment is 100 kg.A dynamics simulation is performed with ADAMS to simulate the actual mo-tion of the motor , provides reasonable reference for motor selection .A dynamic analysis of the key shaft is per-formed with the finite element software ABAQUS for the optimization design of the shaft .The result shows that the swinging mechanism meets the functional requirements .%为设计满足智能脊柱理疗设备功能要求的摆动机构,对比了多种典型的摆动机构特点,设计了主体机构,同时为确保功能实现设计了辅助机构,并在SolidWorks 中构建了整体三维模型.设计最大摆动速度为100次/min,智能脊柱理疗设备最大承重为100kg. 通过ADAMS动力学仿真模拟其真实环境下的运动情况,对电机合理选型;利用ABAQUS对摆动机构关键零件支撑轴进行显式动态分析,优化设计支撑轴. 仿真与分析结果表明,摆动机构满足功能要求.

  9. Mga Kuwento-Kuwento ng Baliw: Lihis na Pagkilos sa Mga Maikling-Kuwentong Tagalog

    OpenAIRE

    Diana Ziganay; Ma. Trinidad Crisanto

    1990-01-01

    Ang sulating ito ay nalikha upang magbigay ng isang pagsusuri at pagtatalakay sa pagbuo ng konsepto ng abnormalidad o kakaibang kilos o pananaw buhat sa mga piling uri ng panitikang Pilipinong naisulat sa wikang Ingles noong ika-1900 hanggang 1945. Pag-uukulan ng pansin dito ang panahon at kalagayang binanggit, ang mga suliraning binanggit, ang maga suliranin ng pagbuo ng panitikang Pilipino sa Ingles, at ang daloy ng kaisipan ng mga manunulat na may kaugnayan sa pag-unawa ng konsepto ng abno...

  10. Mga Kuwento-Kuwento ng Baliw: Lihis na Pagkilos sa Mga Maikling-Kuwentong Tagalog

    Directory of Open Access Journals (Sweden)

    Diana Ziganay

    1990-12-01

    Full Text Available Ang sulating ito ay nalikha upang magbigay ng isang pagsusuri at pagtatalakay sa pagbuo ng konsepto ng abnormalidad o kakaibang kilos o pananaw buhat sa mga piling uri ng panitikang Pilipinong naisulat sa wikang Ingles noong ika-1900 hanggang 1945. Pag-uukulan ng pansin dito ang panahon at kalagayang binanggit, ang mga suliraning binanggit, ang maga suliranin ng pagbuo ng panitikang Pilipino sa Ingles, at ang daloy ng kaisipan ng mga manunulat na may kaugnayan sa pag-unawa ng konsepto ng abnormalidad na sumasalamin sa ilang mga piling sulatin, tuwiran man o di tuwiran.

  11. Analysis and Countermeasures of Drinking Water Contamination Incidents in Shanghai in 2009-2010 MA O Jie, WA NG%2009-2010年上海市生活饮用水突发污染事件分析

    Institute of Scientific and Technical Information of China (English)

    毛洁; 王懿霖; 张怡琼; 应亮

    2011-01-01

    目的 分析上海市2009-2010年生活饮用水突发污染事件发生情况,为提高生活饮用水卫生监督水平提供对策.方法 利用上海市生活饮用水卫生监督预警控制平台收集2009-2010年上海市生活饮用水突发污染事件,并对事件进行统计分析.结果 2009-2010年上海市卫生监督机构共参与调查分布于7个区县的13起生活饮用水突发污染事件,以污染原因分,包括水源水受污染(5起)、自来水管网与其他用途管网相接(4起)、二次供水设施污染(3起)、城市建设对供水管网损坏(1起).以污染环节分,包括水源污染(5起)、管网污染(5起)、二次供水系统污染(3起).结论 上海市饮用水突发污染事件发生原因是多方面的,建议利用上海市生活饮用水卫生监督预警控制平台加强预警;排除污染隐患,从源头预防污染事件;加强监管,规范各类供水单位行为;加强执法人员培训,提升污染事件处置能力.%Objective To analyze drinking water contamination incidents in Shanghai in 2009-2010, and to provide countermeasures for drinking water health inspection proficiency improvement Methods Shanghai drinking water health inspection and warning control platform was used to collect drinking water contamination incidents in Shanghai in 2009-2010,and make analysis was performed based on the platform. Results There were 13 drinking water contamination cases in 7 districts in 2009-2010. According to contamination reasons, the cases can be divided into resource contamination (5 cases),connection of water supply network with other network (4 cases), secondary water supply facilities contamination(3 cases), damage to water supply network by city construction (1 case). According to contamination links, the cases can be divided into resource contamination(5 cases), network contamination(5 cases) and secondary water supply system contamination(3 cases). Conclusion Multiple reasons caused drinking water

  12. Tsapter 17 (Les Bambous de Paris ng Baybayin, l’Alphabet Syllabique des Tagals ni Jean-Paul G. Potet

    Directory of Open Access Journals (Sweden)

    Ramon Guillermo

    2013-12-01

    Full Text Available A comprehensive book on the baybayin writing system was recently published by the French linguist Jean-Paul Potet. One of the most interesting chapters is a transcription and analysis of baybayin inscriptions written on bamboos which are currently in the safekeeping of the Musée du Quai Branly, Paris with inventory numbers 71-1932-11-13 to -20. What follows is a review of Potet’s book and a translation of the chapter on the bamboos of Paris. Lumabas kamakailan lamang ang isang kumprehensibong aklat hinggil sa sistema ng pagsusulat na baybayin ng Pranses na linggwista na si Jean-Paul Potet. Ang isa sa mga pinakainteresanteng tsapter nito ay naglalaman ng isang transkripsiyon at pagsusuri ng mga inskripsiyong baybayin na nakaukit sa mga kawayan na kasalukuyang nasa pangangalaga ng Musée du Quai Branly, Paris kung saan ang bago nilang mga numerong imbentaryo ay 71- 1932-11-13 hanggang -20. Ang sumusunod ay isang rebyu ng aklat ni Potet at pagsasalin ng tsapter hinggil sa mga kawayan ng Paris.

  13. Ang Demokratikong Sistema at ang mga Modelo ng Pamumuno sa Pilipinas

    Directory of Open Access Journals (Sweden)

    F. P. A. Demeterio

    2010-06-01

    Full Text Available Maraming paraan ang pagsusuri sa problema ng pulitika sa Pilipinas.Para sa mga moralista at ideyalista, halimbawa, maaari itong tingnangamit ang perspektibo ng sistema ng pagpapahalaga (values at moralidad; para sa mga materyalista naman, maaari din itong himayin gamit ang perspektibo ng pagka-di-pantay-pantay ng kayamanan at kapangyarihan(unequal wealth and power ng ating mga mamamayan; o hindi kaya para sa mga eksperto sa sistema at istraktura, maaari din itong dalumatin gamit ang perspektibo ng ating marupok na burukrasya. Susubukan ng papel na ito na pag-aralan ang paksa sa pamamagitan ng pagsusuri sa konsepto ng pamumuno na hinihiling ng ating demokratikong sistema at sa kaparehong konseptong umiiiral naman sa kamalayan ng ating mga kababayan. Gagamitin ng pagsusuring ito, bilang teoretikal na balangkas, ang isang uri ng antropolohikalna teorya at pamamaraan na kilala bilang “kognitibong antropolohiya”(cognitive anthropology.

  14. Klaaspärlimäng peletab klassikahirmu / Katrin Nielsen

    Index Scriptorium Estoniae

    Nielsen, Katrin

    2003-01-01

    Eri stiile-ajastuid ühendavast Peeter Vähi kujandatud muusikafestivalist "Klaaspärlimäng" 22. juulist-24. augustini. Kontsertidest Pärnu kontserdimajas: gamelaniansambli Gamelan Son of Lion kontserdist 22. juulil, Vardo Rumesseni kontserdist ja CD esitlusest 28. juulil, tütarlastekoori "Ellerhein" kontserdist 30. juulil

  15. Quasicrystals -- The impact of N.G. de Bruijn

    CERN Document Server

    Au-Yang, Helen

    2013-01-01

    In this paper we put the work of Professor N.G. de Bruijn on quasicrystals in historical context. After briefly discussing what went before, we shall review de Bruijn's work together with recent related theoretical and experimental developments. We conclude with a discussion of Yang-Baxter integrable models on Penrose tilings, for which essential use of de Bruijn's work has been made.

  16. Elu kui näitemäng / Helju Koger

    Index Scriptorium Estoniae

    Koger, Helju, 1943-

    2007-01-01

    VI kihelkonnapäevadest Juurus. Juuru Mihkli kirikus esines ansambel Resonabilis. Konverentsil räägiti Järlepa mõisast, Anu Allikvee pidas ettekande "August von Kotzebue elu nagu näitemäng" jm. Näitemängu "Pärmi Jaagu unenägu" nägi kohalike asjaarmastajate esituses

  17. Mga Lente sa Likod ng Lente: Isang Panimulang Pag-aaral ng Ilang Litratong Kuha ni Xander Angeles

    Directory of Open Access Journals (Sweden)

    Moreal Nagarit Camba

    2011-12-01

    Full Text Available Ang pangalang Xander Angeles ay kilala bilang isa sa mga in-demand napangalan sa mundo ng fashion at advertisement sa Pilipinas. Kakabit ngpangalang ito ang samut-saring lokal at internasyunal na parangal; idagdag pa rito ang isang advertising company, isang modelling agency, at isang fashion and photography school.

  18. Photonic integrated transmitter and receiver for NG-PON2

    Science.gov (United States)

    Tavares, Ana; Lopes, Ana; Rodrigues, Cláudio; Mãocheia, Paulo; Mendes, Tiago; Brandão, Simão.; Rodrigues, Francisco; Ferreira, Ricardo; Teixeira, António

    2014-08-01

    In this paper the authors present a monolithic Photonic Integrated Circuit which includes a transmitter and a receiver for NG-PON2. With this layout it is possible to build an OLT and, by redesigning some filters, also an ONU. This technology allows reducing the losses in the transmitter and in the receiver, increasing power budget, and also reducing the OEO conversions, which has been a major problem that operators want to surpass.

  19. Ang Demokratikong Sistema at ang mga Modelo ng Pamumuno sa Pilipinas

    OpenAIRE

    F. P. A. Demeterio

    2010-01-01

    Maraming paraan ang pagsusuri sa problema ng pulitika sa Pilipinas.Para sa mga moralista at ideyalista, halimbawa, maaari itong tingnangamit ang perspektibo ng sistema ng pagpapahalaga (values) at moralidad; para sa mga materyalista naman, maaari din itong himayin gamit ang perspektibo ng pagka-di-pantay-pantay ng kayamanan at kapangyarihan(unequal wealth and power) ng ating mga mamamayan; o hindi kaya para sa mga eksperto sa sistema at istraktura, maaari din itong dalumatin gamit ang perspek...

  20. Meta Analisis sa Pagsusuri ng Maiikling Kwento sa mga Tesis at Disertasyon

    OpenAIRE

    Regina I. Cuizon

    2014-01-01

    Anumang mga pagbabago na makikita sa mundo ay bunga ng pananaliksik. Ang meta-analisis ay isa sa mga bunga ng pananaliksik na ginagamit sa kasalukuyan bilang teknik upang malaman iba pang mga nagsulputang informasyon. Ito’y pag-aaral sa mga pag-aaral. Isang kritikal at sistematikong pagsusuri sa istruktura ng mga pag-aaral. Maging gabay sa mga gradwadong paaralan sa pagpili ng paksang pagaaralan. Pangunahing layunin na matiyak ang mga pamamaraan sa pagsusuri ng maiikling kwento ng...

  1. Nogo receptor 3, a paralog of Nogo-66 receptor 1 (NgR1),may function as a NgR1 co-receptor for Nogo-66

    Institute of Scientific and Technical Information of China (English)

    Lei Zhang; Xia Kuang; Jian Zhang

    2011-01-01

    Nogo-A is a major myelin associated inhibitor that blocks regeneration of injured axons in the central nervous system (CNS).Nogo-66 (a 66-residue domain of Nogo-A) expressed on the surface of oligodendrocytes has been shown to directly interact with Nogo-66 receptor 1 (NgR1).A number of additional components of NgR1 receptor complex essential for its signaling have been uncovered.However,detailed composition of the complex and its signaling mechanisms remain to be fully elucidated.In this study,we show that Nogo receptor 3 (NgR3),a paralog of NgR1,is a binding protein for NgR1.The interaction is highly specific because other members of the reticulin family,to which Nogo-A belongs,do not bind to NgR3.Neither does NgR3 show any binding activity with Nogo receptor 2 (NgR2),another NgR1 paralog.Majority of NgR3 domains are required for its binding to NgR1.Moreover,a truncated NgR3 with the membrane anchoring domain deleted can function as a decoy receptor to reverse neurite outgrowth inhibition caused by Nogo-66 in culture.These in vitro results,together with previously reported overlapping expression profile between NgR1 and NgR3,suggest that NgR3 may be associated with NgR1 in vivo and that their binding interface may be targeted for treating neuronal injuries.

  2. La frase nominal Ngäbére

    Directory of Open Access Journals (Sweden)

    Murillo Miranda José Manuel

    2009-12-01

    Full Text Available Este trabajo es el avance de una amplia investigación cuyo propósito es elaborar una gramática descriptiva de la lengua chibcha ngäbére. El artículo presenta las características particulares más relevantes de la frase nominal de dicha variante dialectal (hablada en Costa Rica, también en Panamá, y establece la relación con algunas de las características generales de otras lenguas chibchas ístmicas. El primer apartado del escrito es una introducción a la lengua ngäbére y a sus hablantes; elsegundo presenta la morfología de los sustantivos, pronombres y otras clases de palabras que conforman la frase nominal; el tercer apartado trata de la sintaxis de la frase nominal y esboza, de manera sucinta, las relaciones gramaticales; el cuarto apartado es una conclusión valorativa de este trabajo.

  3. Use of Roud Wood in Kaingáng Houses

    Directory of Open Access Journals (Sweden)

    Ricardo Dias Silva

    2002-01-01

    Full Text Available This work has the purpose of describing and analyzing the evolutionary habitation process of the Kaingáng. Its main focus is the typological reading of the shelters as well as the registration of the wooden building system used in the construction of the houses in the “Äpucaraninha” Reserve, located in a district of Londrina. The research proceeds with the elaboration of the genealogy of the kaingáng’s habitation through the consultation to the published historical reports and their chronological organization. The data collection within the group was accomplished through informal interviews, photographic survey and in loco observations. Aspects related to the use of the round wood as well as the other elements that are part of the construction, were verified at this stage. The raw material used in every building system is obtained in the Reserve itself, being the wood used not only in the structure but also in the sealing. The connections are made through fittings and fastenings with liana. In spite of the mixture of values and meanings that the Kaingáng community experiences through the contact with other cultures, traditional aspects related to the building of the “traditional house” are preserved, with no domain of other techniques by most of the group. The registration intends to contribute towards the identification and understanding of important issues related to the spaces built by the man.

  4. Anionic chemistry of noble gases: formation of Mg-NG (NG = Xe, Kr, Ar) compounds under pressure.

    Science.gov (United States)

    Miao, Mao-Sheng; Wang, Xiao-Li; Brgoch, Jakoah; Spera, Frank; Jackson, Matthew G; Kresse, Georg; Lin, Hai-Qing

    2015-11-11

    While often considered to be chemically inert, the reactivity of noble gas elements at elevated pressures is an important aspect of fundamental chemistry. The discovery of Xe oxidation transformed the doctrinal boundary of chemistry by showing that a complete electron shell is not inert to reaction. However, the reductive propensity, i.e., gaining electrons and forming anions, has not been proposed or examined for noble gas elements. In this work, we demonstrate, using first-principles electronic structure calculations coupled to an efficient structure prediction method, that Xe, Kr, and Ar can form thermodynamically stable compounds with Mg at high pressure (≥125, ≥250, and ≥250 GPa, respectively). The resulting compounds are metallic and the noble gas atoms are negatively charged, suggesting that chemical species with a completely filled shell can gain electrons, filling their outermost shell(s). Moreover, this work indicates that Mg2NG (NG = Xe, Kr, Ar) are high-pressure electrides with some of the electrons localized at interstitial sites enclosed by the surrounding atoms. Previous predictions showed that such electrides only form in Mg and its compounds at very high pressures (>500 GPa). These calculations also demonstrate strong chemical interactions between the Xe 5d orbitals and the quantized interstitial quasiatom (ISQ) orbitals, including the strong chemical bonding and electron transfer, revealing the chemical nature of the ISQ. PMID:26488848

  5. Anionic chemistry of noble gases: formation of Mg-NG (NG = Xe, Kr, Ar) compounds under pressure.

    Science.gov (United States)

    Miao, Mao-Sheng; Wang, Xiao-Li; Brgoch, Jakoah; Spera, Frank; Jackson, Matthew G; Kresse, Georg; Lin, Hai-Qing

    2015-11-11

    While often considered to be chemically inert, the reactivity of noble gas elements at elevated pressures is an important aspect of fundamental chemistry. The discovery of Xe oxidation transformed the doctrinal boundary of chemistry by showing that a complete electron shell is not inert to reaction. However, the reductive propensity, i.e., gaining electrons and forming anions, has not been proposed or examined for noble gas elements. In this work, we demonstrate, using first-principles electronic structure calculations coupled to an efficient structure prediction method, that Xe, Kr, and Ar can form thermodynamically stable compounds with Mg at high pressure (≥125, ≥250, and ≥250 GPa, respectively). The resulting compounds are metallic and the noble gas atoms are negatively charged, suggesting that chemical species with a completely filled shell can gain electrons, filling their outermost shell(s). Moreover, this work indicates that Mg2NG (NG = Xe, Kr, Ar) are high-pressure electrides with some of the electrons localized at interstitial sites enclosed by the surrounding atoms. Previous predictions showed that such electrides only form in Mg and its compounds at very high pressures (>500 GPa). These calculations also demonstrate strong chemical interactions between the Xe 5d orbitals and the quantized interstitial quasiatom (ISQ) orbitals, including the strong chemical bonding and electron transfer, revealing the chemical nature of the ISQ.

  6. Recce NG: from Recce sensor to image intelligence (IMINT)

    Science.gov (United States)

    Larroque, Serge

    2001-12-01

    Recce NG (Reconnaissance New Generation) is presented as a complete and optimized Tactical Reconnaissance System. Based on a new generation Pod integrating high resolution Dual Band sensors, the system has been designed with the operational lessons learnt from the last Peace Keeping Operations in Bosnia and Kosovo. The technical solutions retained as component modules of a full IMINT acquisition system, take benefit of the state of art in the following key technologies: Advanced Mission Planning System for long range stand-off Manned Recce, Aircraft and/or Pod tasking, operating sophisticated back-up software tools, high resolution 3D geo data and improved/combat proven MMI to reduce planning delays, Mature Dual Band sensors technology to achieve the Day and Night Recce Mission, including advanced automatic operational functions, as azimuth and roll tracking capabilities, low risk in Pod integration and in carrier avionics, controls and displays upgrades, to save time in operational turn over and maintenance, High rate Imagery Down Link, for Real Time or Near Real Time transmission, fully compatible with STANAG 7085 requirements, Advanced IMINT Exploitation Ground Segment, combat proven, NATO interoperable (STANAG 7023), integrating high value software tools for accurate location, improved radiometric image processing and open link to the C4ISR systems. The choice of an industrial Prime contractor mastering across the full system, all the prior listed key products and technologies, is mandatory to a successful delivery in terms of low Cost, Risk and Time Schedule.

  7. Isang Reaksyon sa “Ang Demokratikong Sistema at ang mga Modelo ng Pamumuno sa Pilipinas”

    Directory of Open Access Journals (Sweden)

    Paolo A. Bolanos

    2010-06-01

    Full Text Available Kinukundisyon ng nalalapit na eleksyon sa Mayo ang ating kolektibongpag-iisip bilang mamamayan ng Pilipinas. Bahagyang hinuhubog nitoang ating kilos at salita na syang kumakatawan sa ating pag-asa at mgapangarap para sa ating sarili at para sa ating bansa. Dahil nakatuon ang atensyon natin sa nalalapit na eleksyon at dahil hindi maiwasang pag-usapan ang mga pambansang isyu na kadalasa’y mga pambansang suliranin (tulad ng kurapsyon at kahirapan, nagkakaroon tayo ng natatangi at mas malawak na pag-mumuni-muni sa ating sosyoholikal at politikal na sitwasyon, na tila namang nambubuyo sa ating pakiramdam ng kabiguan. Talaga naman na ang panahon ng eleksyon ay parehong panahon ng pag-asa at kabiguan—kadalasa’y ang pangalawa ang nananatiling realidad ng mga Pilipino!

  8. Salman Rushdie: "Troonide mäng" on rämps

    Index Scriptorium Estoniae

    2011-01-01

    Oma esimest telesarja "Järgmised inimesed" kirjutav Salman Rushdie ei hinda USA telekanali HBO seriaali "Troonide mäng" (George R. R. Martini "Jää ja tule laulu" sarja esimese raamatu "Troonide mäng" teleekraniseering)

  9. Isang Reaksyon sa “Ang Demokratikong Sistema at ang mga Modelo ng Pamumuno sa Pilipinas”

    OpenAIRE

    Paolo A. Bolanos

    2010-01-01

    Kinukundisyon ng nalalapit na eleksyon sa Mayo ang ating kolektibongpag-iisip bilang mamamayan ng Pilipinas. Bahagyang hinuhubog nitoang ating kilos at salita na syang kumakatawan sa ating pag-asa at mgapangarap para sa ating sarili at para sa ating bansa. Dahil nakatuon ang atensyon natin sa nalalapit na eleksyon at dahil hindi maiwasang pag-usapan ang mga pambansang isyu na kadalasa’y mga pambansang suliranin (tulad ng kurapsyon at kahirapan), nagkakaroon tayo ng natatangi at mas malawak na...

  10. Meta Analisis sa Pagsusuri ng Maiikling Kwento sa mga Tesis at Disertasyon

    Directory of Open Access Journals (Sweden)

    Regina I. Cuizon

    2014-10-01

    Full Text Available Anumang mga pagbabago na makikita sa mundo ay bunga ng pananaliksik. Ang meta-analisis ay isa sa mga bunga ng pananaliksik na ginagamit sa kasalukuyan bilang teknik upang malaman iba pang mga nagsulputang informasyon. Ito’y pag-aaral sa mga pag-aaral. Isang kritikal at sistematikong pagsusuri sa istruktura ng mga pag-aaral. Maging gabay sa mga gradwadong paaralan sa pagpili ng paksang pagaaralan. Pangunahing layunin na matiyak ang mga pamamaraan sa pagsusuri ng maiikling kwento ng mga tesis at disertasyong nagawa mula sa mga piling SUCs. Desinyong kwalitatibo - kontent analisis sa pagsusuri ng: kaanyuan ayon sa suliranin, metodolohiya, paglalahad at interpretasyon ng mga datos, natuklasan, konklusyon, at rekomendasyon; kahinatnan ayon sa pagkatulad, pagkakaiba at kabuluhan; Emerging tema. Napag-alaman na ang karaniwang pinag-aralan ay 30% kahalagahang pangkatauhan, 20% larawangdiwa ng mga kababaihan at 10% gramatikang aspeto. Sa metodolohiyang ginamit, 80% desinyong kwalitatibo at 20% kwantitatibo-kwalitatibo. Sa paglikom ng datos 60% diretsahang pagsusuri, 30% talatanungan at 10% tseklis. Sa pag-analisa 90% kontent analisis at 10% gramatikal analisis. Batay sa natuklasan, ang pagsusuri ng maiikling kwento sa mga tesis at disertasyon mula sa iba’t ibang paaralan gamit ang meta analisis ay isang epektibo, mabisa, objektibong paraan at kagamitan na magagamit sa makatarungang paghatol; pamumuna sa kabuluhan at kagandahan; paghaham- bing sa mga kritikal na isyu; at pormulasyon ng panibagong pamantayan at batas.

  11. 76 FR 12627 - Airworthiness Directives; Diamond Aircraft Industries GmbH Models DA 42, DA 42 NG, and DA 42 M-NG...

    Science.gov (United States)

    2011-03-08

    ... the MCAI, on November 23, 2010, we issued AD 2010-25-01, Amendment 39-16534 (75 FR 75868, December 7... Order 12866; 2. Is not a ``significant rule'' under the DOT Regulatory Policies and Procedures (44 FR... Industries GmbH Models DA 42, DA 42 NG, and DA 42 M-NG Airplanes AGENCY: Federal Aviation Administration...

  12. NG2细胞与中枢神经系统疾病%Roles of NG2 glial cells in diseases of the central nervous system

    Institute of Scientific and Technical Information of China (English)

    许建平; 赵杰; 李韶

    2011-01-01

    NG2 cells are a novel distinct class of central nervous system(CNS)glial cells,characterized by the expression of the chondroitin sulfate proteoglycan NG2.They have been detected in a variety of human CNS diseases.As morphological,physiological and biomolecular studies of NG2 cells have been conducted,their roles have been gradually revealed.Research on cellular and molecular mechanisms in the pathophysiological state was built on the preliminary findings of their physiological functions; and in turn,this helps to clarify their physiological roles and leads to the identification of novel therapeutic targets.This review summarizes recent findings regarding the potential roles of NG2 cells in traumatic brain injury,multiple sclerosis,glioma,epilepsy,Alzheimer's disease and electroconvulsive therapy for depression.%NG2细胞是新发现的一类广泛存在于成熟和发育期中枢神经系统的胶质细胞群体.这些细胞表面表达NG2硫酸软骨素蛋白多糖,因而常被称作NG2细胞.随着NG2细胞形态学研究的深入,NG2胶质细胞的功能也越来越受到关注.NG2细胞在人类多种中枢神经系统疾病中扮演重要角色.本文结合最新的研究报道,就其在一些常见的中枢神经系统疾病中的作用进行概括综述.

  13. Study on Interaction of ADN and (NC+NG) by Gasometric Method%量气法研究ADN与(NC+NG)的相互作用

    Institute of Scientific and Technical Information of China (English)

    何少蓉; 张林军; 衡淑云; 刘子如

    2008-01-01

    用 NBK型"拉瓦"量气测试系统研究了较高温度下二硝酰胺铵(ADN)与双基吸收药(NC+NG)体系的相互作用,从动力学角度阐述了二者的相互作用.结果表明: ADN与(NC+NG)混合物加热分解的最终放气量在标准状态下为626.8 mL·g-1,与ADN和(NC+NG)最大放气量之和相比并没有增加,但分解速度显著提高,活化能显著降低至82.58 kJ·mol-1,ADN与(NC+NG)之间存在强烈的相互作用.

  14. Basin-scale characterization of river hydromorphology by map derived information: A case study on the Red River (Sôngng), Vietnam

    Science.gov (United States)

    Schmitt, R. J.; Bizzi, S.; Castelletti, A.

    2012-12-01

    The understanding of river hydromorphological processes has been recognized in the last decades as a priority of modern catchment management, since fluvial geomorphic processes shape physical habitat, affect river infrastructures and influence freshwater ecological processes. Characterization of river hydromorphological features is commonly location specific and highly demanding in terms of field-works, resource and expertise required. Therefore, its routine application at regional or national scales, although an urgent need of catchment management, is infeasible at present. Recently available high-resolution data, such as DEM or LIDAR, opens up novel potential for basin-wide analysis of fluvial processes at limited effort and cost. Specifically, in this study we assess the feasibility of characterizing river hydromorphology from specific map derived geomorphic controls namely: channel gradient, bankfull flow, specific stream power, and degree of channel confinement. The river network, extracted from a digital elevation model and validated with available network shape-files and optical satellite imagery, available flow gauging stations and GIS processing allow producing continuous values of geomorphic drivers defined over given length segments at catchment or regional scales. This generic framework was applied to the Red River (Sôngng) basin, the second largest basin (87,800 km2) in Vietnam. Besides its economic importance, the river since few years is experiencing severe river bed incisions due to the building of new dams in the upstream part of the catchment and sand mining in the surrounding of the capital city Hanoi. In this context, characterized by an high developing rate, current efforts to increase water productivity by infrastructure and management measures require a thorough understanding of fluvial system and, in particular, of the basin-wide river hydromorphology. The framework proposed has allowed producing high-dimensional samples of spatially

  15. Determination of fragrances at ng/L levels using CLSA and GC/MS detection.

    Science.gov (United States)

    Mitjans, D; Ventura, F

    2005-01-01

    Polycyclic and nitro musks and two fragrances (Acetyl cedrene and Amberonne) have been determined and quantified in influent and effluent waste water, river water and tap water samples from different European countries by closed loop stripping analysis (CLSA) as a method of preconcentration and GC/MS operating in the SIM mode. Limits of detection; precision expressed as repeatability and reproducibility relative standard deviations of the method; matrix effects and the estimation of the uncertainty have been evaluated. All samples contained different musks at ng/l levels being the polycyclic musks Galaxolide and Tonalide and both fragrances, Amberonne and Acetyl cedrene the most abundant. Removal of these main compounds is achieved partially in all waste water treatment plants studied. These results suggest the importance of studying and controlling the presence of these ubiquitous environmental compounds in water systems. PMID:16459786

  16. NG2 targets tumorigenic Rb inactivation in Pit1-lineage pituitary cells.

    Science.gov (United States)

    Tateno, Toru; Nakano-Tateno, Tae; Ezzat, Shereen; Asa, Sylvia L

    2016-05-01

    The proteoglycan neuron-glial antigen 2 (NG2) is expressed by oligodendrocyte progenitors, pericytes, and some cancerous cells where it is implicated in tumor development. We examined mice with NG2-driven pRb inactivation. Unexpectedly, NG2-Cre:pRb(flox/flox) mice developed pituitary tumors with high penetrance. Adenohypophysial neoplasms developed initially as multifocal lesions; by 1 year, large tumors showed brain invasion. Immunohistochemistry identified these as Pit1-lineage neoplasms, with variable immunoreactivity for growth hormone, prolactin, thyrotropin, and α-subunit of glycoprotein hormones. Other than modest hyperprolactinemia, circulating hormone levels were not elevated. To determine the role of NG2 in the pituitary, we investigated NG2 expression. Immunoreactivity was identified in anterior and posterior lobes but not in the intermediate lobe of the mouse pituitary; in the adenohypophysis, folliculostellate cells had the strongest NG2 immunoreactivity but showed no proliferation in response to Rb inactivation. Pit1-positive adenohypophysial cells were positive for NG2, but corticotroph and gonadotroph cells were negative. RT-PCR revealed NG2 expression in normal human pituitary and human pituitary tumors; immunohistochemistry localized NG2 in nontumorous human adenohypophysis with strongest positivity in folliculostellate cells, and in tumors of all types except corticotrophs. Functional studies in GH4 mammosomatotrophs showed that NG2 increases prolactin (PRL), reduces growth hormone (GH) expression, and enhances cell adhesion without influencing proliferation. In conclusion, NG2-driven pRb inactivation results in pituitary tumors that mimic endocrinologically inactive Pit1-lineage human pituitary tumors. This model identifies a role for NG2 in pituitary cell-type-specific functions and unmasks a protective role from Rb inactivation in folliculostellate cells; it can be used for further research, including preclinical testing of novel therapies

  17. Differential phosphorylation of NG2 proteoglycan by ERK and PKCα helps balance cell proliferation and migration

    Science.gov (United States)

    Makagiansar, Irwan T.; Williams, Scott; Mustelin, Tomas; Stallcup, William B.

    2007-01-01

    Two distinct Thr phosphorylation events within the cytoplasmic domain of the NG2 proteoglycan help regulate the cellular balance between proliferation and motility. Protein kinase Cα mediates the phosphorylation of NG2 at Thr2256, resulting in enhanced cell motility. Extracellular signal–regulated kinase phosphorylates NG2 at Thr2314, stimulating cell proliferation. The effects of NG2 phosphorylation on proliferation and motility are dependent on β1-integrin activation. Differential cell surface localization of the two distinctly phosphorylated forms of NG2 may be the mechanism by which the NG2–β1-integrin interaction promotes proliferation in one case and motility in the other. NG2 phosphorylated at Thr2314 colocalizes with β1-integrin on microprotrusions from the apical cell surface. In contrast, NG2 phosphorylated at Thr2256 colocalizes with β1-integrin on lamellipodia at the leading edges of cells. Thus, phosphorylation and the resulting site of NG2–integrin localization may determine the specific downstream effects of integrin signaling. PMID:17591920

  18. Differential phosphorylation of NG2 proteoglycan by ERK and PKCalpha helps balance cell proliferation and migration.

    Science.gov (United States)

    Makagiansar, Irwan T; Williams, Scott; Mustelin, Tomas; Stallcup, William B

    2007-07-01

    Two distinct Thr phosphorylation events within the cytoplasmic domain of the NG2 proteoglycan help regulate the cellular balance between proliferation and motility. Protein kinase Calpha mediates the phosphorylation of NG2 at Thr2256, resulting in enhanced cell motility. Extracellular signal-regulated kinase phosphorylates NG2 at Thr2314, stimulating cell proliferation. The effects of NG2 phosphorylation on proliferation and motility are dependent on beta1-integrin activation. Differential cell surface localization of the two distinctly phosphorylated forms of NG2 may be the mechanism by which the NG2-beta1-integrin interaction promotes proliferation in one case and motility in the other. NG2 phosphorylated at Thr2314 colocalizes with beta1-integrin on microprotrusions from the apical cell surface. In contrast, NG2 phosphorylated at Thr2256 colocalizes with beta1-integrin on lamellipodia at the leading edges of cells. Thus, phosphorylation and the resulting site of NG2-integrin localization may determine the specific downstream effects of integrin signaling.

  19. Role of NG2 Expressing Cells in Addiction: A New approach for an Old Problem

    Directory of Open Access Journals (Sweden)

    Sucharita eSomkuwar

    2014-12-01

    Full Text Available Neuron-glial antigen 2 (NG2 is a proteoglycan expressed predominantly in oligodendrocyte progenitor cells (OPCs. NG2-expressing OPCs (NG2-OPCs are self-renewing cells that are widely distributed in the grey and white matter areas of the central nervous system. NG2-OPCs can mature into premyelinating oligodendrocytes and myelinating oligodendroglia which serve as the primary source of myelin in the brain. This review characterizes NG2-OPCs in brain structure and function, conceptualizes the role of NG2-OPCs in brain regions associated with negative reinforcement and relapse to drug seeking and discusses how NG2-OPCs are regulated by neuromodulators linked to motivational withdrawal. We hope to provide the readers with an overview of the role of NG2-OPCs in brain structure and function in the context of negative affect state in substance abuse disorders and to integrate our current understanding of the physiological significance of the NG2-OPCs in the adult brain.

  20. Polityka językowa Rumunii a dialekt csángó

    OpenAIRE

    Kozakiewicz, Joanna

    2012-01-01

    Abstract (Language policy of Romania and the dialect csángó). Csángós are an ethnic group of Hungarian origin, settled in Moldavian part of Romania at the end of 13th century. Over the centuries, they have lived in isolation which enabled preservation of their archaic dialect and culture. Today the bilingual Csángó speakers are on the verge of language shift to Romanian. The paper, apart from introducing the features of the endangered Csángó dialect, presents the relation between the Romanian...

  1. "Troonide mäng" toob fantaasia usutavalt teleekraanile / Mari Rebane

    Index Scriptorium Estoniae

    Rebane, Mari

    2011-01-01

    USA telekanali HBO telesarjast "Troonide mäng", George R. R. Martini "Jää ja tule laulu" sarja esimese raamatu "Troonide mäng" teleekraniseeringust. Sarja esimene osa esilinastus USA-s 17. aprillil. Osades Sean Bean jt.

  2. 76 FR 55293 - Special Conditions: Diamond Aircraft Industries, Model DA-40NG; Electronic Engine Control (EEC...

    Science.gov (United States)

    2011-09-07

    ... TRANSPORTATION Federal Aviation Administration 14 CFR Part 23 Special Conditions: Diamond Aircraft Industries, Model DA-40NG; Electronic Engine Control (EEC) System AGENCY: Federal Aviation Administration (FAA), DOT... Diamond Aircraft Industries (DAI), model DA-40NG airplane. This airplane will have a novel or...

  3. 76 FR 19903 - Special Conditions: Diamond Aircraft Industry Model DA-40NG; Diesel Cycle Engine

    Science.gov (United States)

    2011-04-11

    ... Conditions: Diamond Aircraft Industry Model DA-40NG; Diesel Cycle Engine AGENCY: Federal Aviation... conditions are issued for the Diamond Aircraft Industry (DAI) GmbH model DA-40NG the Austro Engine GmbH model E4 aircraft diesel engine (ADE) using turbine (jet) fuel. This airplane will have a novel or...

  4. Kuuldemäng "Palju õnne argipäevaks!" edukümnes

    Index Scriptorium Estoniae

    2006-01-01

    Raadioteatri kuuldemäng "Palju õnne argipäevaks" jõudis Euroopa meediafestivalil Prix Europa kümne parema hulka. Näidendi lavastas raadio jaoks Andres Noormets. 1999. a. jõudis festivalil Prix Europa äramärkimiseni Ervin Õunapuu kuuldemäng "Õhtueine Emmauses"

  5. Reduced inflammation accompanies diminished myelin damage and repair in the NG2 null mouse spinal cord

    Directory of Open Access Journals (Sweden)

    Kucharova Karolina

    2011-11-01

    Full Text Available Abstract Background Multiple sclerosis (MS is a demyelinating disease in which blood-derived immune cells and activated microglia damage myelin in the central nervous system. While oligodendrocyte progenitor cells (OPCs are essential for generating oligodendrocytes for myelin repair, other cell types also participate in the damage and repair processes. The NG2 proteoglycan is expressed by OPCs, pericytes, and macrophages/microglia. In this report we investigate the effects of NG2 on these cell types during spinal cord demyelination/remyelination. Methods Demyelinated lesions were created by microinjecting 1% lysolecithin into the lumbar spinal cord. Following demyelination, NG2 expression patterns in wild type mice were studied via immunostaining. Immunolabeling was also used in wild type and NG2 null mice to compare the extent of myelin damage, the kinetics of myelin repair, and the respective responses of OPCs, pericytes, and macrophages/microglia. Cell proliferation was quantified by studies of BrdU incorporation, and cytokine expression levels were evaluated using qRT-PCR. Results The initial volume of spinal cord demyelination in wild type mice is twice as large as in NG2 null mice. However, over the ensuing 5 weeks there is a 6-fold improvement in myelination in wild type mice, versus only a 2-fold improvement in NG2 null mice. NG2 ablation also results in reduced numbers of each of the three affected cell types. BrdU incorporation studies reveal that reduced cell proliferation is an important factor underlying NG2-dependent decreases in each of the three key cell populations. In addition, NG2 ablation reduces macrophage/microglial cell migration and shifts cytokine expression from a pro-inflammatory to anti-inflammatory phenotype. Conclusions Loss of NG2 expression leads to decreased proliferation of OPCs, pericytes, and macrophages/microglia, reducing the abundance of all three cell types in demyelinated spinal cord lesions. As a result

  6. Hur uppnås framgång på lång sikt med varumärkesutvidgningar? : En fallstudie av Hennes & Mauritz

    OpenAIRE

    Medanhodzic, Nejra; Karamehmedovic, Nadina

    2013-01-01

    Sammanfattning – ”Hur uppnås framgång på lång sikt med varumärkesutvidgningar?” Frågeställning: - Hur kan Hennes & Mauritz's varumärkesutvidgning studeras och beskrivas för att ett företag inom klädbranschen ska kunna ta del av denna information och lyckas utvidga framgångsrikt på lång sikt?- Hur ställer sig konsumenter mot utökningar av ett varumärke? Syfte:         Syftet med denna uppsats är att studera varumärkesutvidgning ur ett långsiktigt perspektiv genom att analysera företaget He...

  7. Axonal degeneration stimulates the formation of NG2+ cells and oligodendrocytes in the mouse

    DEFF Research Database (Denmark)

    Nielsen, Helle Hvilsted; Ladeby, Rune; Drøjdahl, Nina;

    2006-01-01

    Proliferation of the adult NG2-expressing oligodendrocyte precursor cells has traditionally been viewed as a remyelination response ensuing from destruction of myelin and oligodendrocytes, and not to the axonal pathology that is also a characteristic of demyelinating disease. To better understand...... the response of the NG2+ cells to the different components of demyelinating pathology, we investigated the response of adult NG2+ cells to axonal degeneration in the absence of primary myelin or oligodendrocyte pathology. Axonal degeneration was induced in the hippocampal dentate gyrus of adult mice...... by transection of the entorhino-dentate perforant path projection. The acutely induced degeneration of axons and terminals resulted in a prompt response of NG2+ cells, consisting of morphological transformation, cellular proliferation, and upregulation of NG2 expression days 2-3 after surgery. This was followed...

  8. Ang Social Network sa Facebook ng mga Taga-Batangas at ng mga Taga-Laguna: Isang Paghahambing

    Directory of Open Access Journals (Sweden)

    Jaderick P. Pabico

    2013-12-01

    Full Text Available Online social networking (OSN has become of great influence to Filipinos, where Facebook, Twitter, LinkedIn, Google+, and Instagram are among the popular ones. Their popularity, coupled with their intuitive and interactive use, allow one's personal information such as gender, age, address, relationship status, and list of friends to become publicly available. The accessibility of information from these sites allow, with the aid of computers, for the study of a wide population's characteristics even in a provincial scale. Aside from being neighbouring locales, the respective residents of Laguna and Batangas both derive their livelihoods from two lakes, Laguna de Bay and Taal Lake. Both residents experience similar problems, such as that, among many others, of fish kill. The goal of this research is to find out similarities in their respective online populations, particularly that of Facebook's. With the use of computational dynamic social network analysis (CDSNA, we found out that the two communities are similar, among others, as follows: both populations are dominated by single young female; Homophily was observed when choosing a friend in terms of age (i.e., friendships were created more often between people whose ages do not differ by at most five years; and Heterophily was observed when choosing friends in terms of gender (i.e., more friendships were created between a male and a female than between both people of the same gender. This paper also presents the differences in the structure of the two social networks, such as degrees of separation and preferential attachment.

  9. Kakaibang Halimuyak: Iba't Ibang Imahe ng Pangangapital sa Balintawak

    Directory of Open Access Journals (Sweden)

    Mariah Amour C. Miranda

    2015-06-01

    Full Text Available Ginagalugad ng sanaysay na ito ang isang pangunahing lugar na nadaraanan mula Norte patungong Maynila—ang Balintawak. Nagsisilbing manibela ng pagsusuri ang kontemporaneong pananaw sa lunan bilang pugad ng mga anyong may magkakasalungat na kahulugan. Binibigyang-pansin ng sanaysay ang iba't ibang imahe ng pagbebenta na matatagpuan sa Balintawak: ang billboard ng kumpanyang Bench na siyang pinakamalaki, ang mismong palengke, at ang mga lakong paninda sa tabing-kalsada. Sa pamamagitan nito, maipapakita ng sanaysay kung paano madadalumat ang Balintawak bilang isang panlipunang texto na sumasalamin sa mga magkakaibang pangangapital sa naturang lunan. This essay explores the space of Balintawak—a focal point of urban travel from northern Luzon to Manila. Using the contemporary view on space as a site of structures with contradictory meanings, the study seeks to understand how various images of market activities in Balintawak create meaningful representations. These images include the gigantic billboard of Bench, the central market itself, and flea markets in the area. The essay shows how Balintawak as a social text serves as a trading center of different forms of capital.

  10. Measurement of serum zinc improves prostate cancer detection efficiency in patients with PSA levels between 4 ng/mL and 10 ng/mL

    Institute of Scientific and Technical Information of China (English)

    Xiao-Meng Li; Ling Zhang; Jiang Li; Yang Li; Hong-Liang Wang; Guo-Yi Ji; Masaaki Kuwahara; Xue-Jian Zhao

    2005-01-01

    Aim: To investigate whether the measurement of serum zinc may improve the detection of prostate cancer (Pca) in men who had total prostate-specific antigen (PSA) levels higher than 4.1 ng/mL. Methods: A mass screening for Pca of 3940 men over S0 years old was undertaken using total serum PSA. Of the 190 men (4.8 %) with elevated PS A, 143(3.6 %) underwent a transrectal ultrasonography (TRUS)-guided biopsy of the prostate, and 42 men (1% of total and 29.3 % of men undergoing biopsy) were found to have cancer. The areas under the receiver operating characteristic curves (ROC-AUC) were used to compare the diagnostic power of cancer detection by means of serum zinc, and free PSA/total PSA ratio (f/t). Results: The men with levels of serum zinc that ranged from 40 ng/mL-60 ng/mL, had an age-adjusted odds ratios(OR) of 5.0. A cutoff value of 100 μg/mL forserum zinc concentration provided a sensitivity of 90.5 % and a specificity of 32.7 % in elevated PSA range, and a sensitivity of 93.3 % and specificity of 27.1% in gray zone, respectively. In the gray zone ranges of 4.1 ng/mL-10.0 ng/mL, the ROC-AUC for zinc was 73.0 %higher than 62.7 % of f/t PSA ratio and 56.7 % of total PSA. Conclusion: Pca displays a lower serum zinc concentration. The measurement of zinc levels improves Pca detection in the gray zone compared with the f/t PSA ratio and total PSA.

  11. Generation of reactive astrocytes from NG2 cells is regulated by sonic hedgehog.

    Science.gov (United States)

    Honsa, Pavel; Valny, Martin; Kriska, Jan; Matuskova, Hana; Harantova, Lenka; Kirdajova, Denisa; Valihrach, Lukas; Androvic, Peter; Kubista, Mikael; Anderova, Miroslava

    2016-09-01

    NG2 cells, a fourth glial cell type in the adult mammalian central nervous system, produce oligodendrocytes in the healthy nervous tissue, and display wide differentiation potential under pathological conditions, where they could give rise to reactive astrocytes. The factors that control the differentiation of NG2 cells after focal cerebral ischemia (FCI) are largely unknown. Here, we used transgenic Cspg4-cre/Esr1/ROSA26Sortm14(CAG-tdTomato) mice, in which tamoxifen administration triggers the expression of red fluorescent protein (tomato) specifically in NG2 cells and cells derived therefrom. Differentiation potential (in vitro and in vivo) of tomato-positive NG2 cells from control or postischemic brains was determined using the immunohistochemistry, single cell RT-qPCR and patch-clamp method. The ischemic injury was induced by middle cerebral artery occlusion, a model of FCI. Using genetic fate-mapping method, we identified sonic hedgehog (Shh) as an important factor that influences differentiation of NG2 cells into astrocytes in vitro. We also manipulated Shh signaling in the adult mouse brain after FCI. Shh signaling activation significantly increased the number of astrocytes derived from NG2 cells in the glial scar around the ischemic lesion, while Shh signaling inhibition caused the opposite effect. Since Shh signaling modifications did not change the proliferation rate of NG2 cells, we can conclude that Shh has a direct influence on the differentiation of NG2 cells and therefore, on the formation and composition of a glial scar, which consequently affects the degree of the brain damage. GLIA 2016;64:1518-1531. PMID:27340757

  12. Chiral pharmacokinetics and inversion of NG-nitro-arginine in the rat

    Institute of Scientific and Technical Information of China (English)

    Yan-feiXIN; RuiTONG; YangFANG; Xiang-junZHOU; Yong-xiangWANG

    2004-01-01

    AIM: To explore pharmacokinetics of NG-nitro-D-arginine (D-NNA) and NG-nitro-L-arginine (L-NNA) in conscious rats.METHODS: The plasma concentration of D-NNA and L-NNA were determined by chiral ligand exchange method with capillary electrochromatography (CEC). Pharmacokinetic parameters were estimated using non-compartment model and were fitted using a computer program DAS. Chiral inversion rate of D-NNA to L-

  13. TIẾP CẬN NHÂN HỌC TRONG NGHIÊN CỨU VỀ NGUỒN GỐC CƯ DÂN HUẾ VÀ VÙNG PHỤ CẬNNHỮNG MẢNG MÀU LÀM NÊN SẮC THÁI VĂN HÓA VÙNG ĐẤT NÚI NGỰ SÔNG HƯƠNG

    OpenAIRE

    Nguyen Xuan, Hong; Nguyễn Xuân, Hồng

    2010-01-01

    VĂN HÓA - LỊCH SỬ HUẾ QUA GÓC NHÌN LÀNG XÃ PHỤ CẬN VÀ QUAN HỆ VỚI BÊN NGOÀI Session 2: Văn hoá - lịch sử Huế và phụ cận フエの文化と歴史:周辺集落と外部との関係からの視点より Session 2: Culture - history of Hue and its surroundings フエとその周辺の文化・歴史

  14. Life Cycle GHG of NG-Based Fuel and Electric Vehicle in China

    Directory of Open Access Journals (Sweden)

    Qian Zhang

    2013-05-01

    Full Text Available This paper compares the greenhouse gas (GHG emissions of natural gas (NG- based fuels to the GHG emissions of electric vehicles (EVs powered with NG-to-electricity in China. A life-cycle model is used to account for full fuel cycle and use-phase emissions, as well as vehicle cycle and battery manufacturing. The reduction of life-cycle GHG emissions of EVs charged by electricity generated from NG, without utilizing carbon dioxide capture and storage (CCS technology can be 36%–47% when compared to gasoline vehicles. The large range change in emissions reduction potential is driven by the different generation technologies that could in the future be used to generate electricity in China. When CCS is employed in power plants, the GHG emission reductions increase to about 71%–73% compared to gasoline vehicles. It is found that compressed NG (CNG and liquefied NG (LNG fuels can save about 10% of carbon as compared to gasoline vehicles. However, gas-to-liquid (GTL fuel made through the Fischer-Tropsch method will likely lead to a life-cycle GHG emissions increase, potentially 3%–15% higher than gasoline, but roughly equal to petroleum-based diesel. When CCS is utilized, the GTL fueled vehicles emit roughly equal GHG emissions to petroleum-based diesel fuel high-efficient hybrid electric vehicle from the life-cycle perspective.

  15. The novel hydroxylamine derivative NG-094 suppresses polyglutamine protein toxicity in Caenorhabditis elegans.

    Science.gov (United States)

    Haldimann, Pierre; Muriset, Maude; Vígh, László; Goloubinoff, Pierre

    2011-05-27

    Aggregation-prone polyglutamine (polyQ) expansion proteins cause several neurodegenerative disorders, including Huntington disease. The pharmacological activation of cellular stress responses could be a new strategy to combat protein conformational diseases. Hydroxylamine derivatives act as co-inducers of heat-shock proteins (HSPs) and can enhance HSP expression in diseased cells, without significant adverse effects. Here, we used Caenorhabditis elegans expressing polyQ expansions with 35 glutamines fused to the yellow fluorescent protein (Q35-YFP) in body wall muscle cells as a model system to investigate the effects of treatment with a novel hydroxylamine derivative, NG-094, on the progression of polyQ diseases. NG-094 significantly ameliorated polyQ-mediated animal paralysis, reduced the number of Q35-YFP aggregates and delayed polyQ-dependent acceleration of aging. Micromolar concentrations of NG-094 in animal tissues with only marginal effects on the nematode fitness sufficed to confer protection against polyQ proteotoxicity, even when the drug was administered after disease onset. NG-094 did not reduce insulin/insulin-like growth factor 1-like signaling, but conferred cytoprotection by a mechanism involving the heat-shock transcription factor HSF-1 that potentiated the expression of stress-inducible HSPs. NG-094 is thus a promising candidate for tests on mammalian models of polyQ and other protein conformational diseases. PMID:21471208

  16. SNOW AVALANCHE ACTIVITY IN PARÂNG SKI AREA REVEALED BY TREE-RINGS

    Directory of Open Access Journals (Sweden)

    F. MESEȘAN

    2014-11-01

    Full Text Available Snow Avalanche Activity in Parâng Ski Area Revealed by Tree-Rings. Snow avalanches hold favorable conditions to manifest in Parâng Mountains but only one event is historically known, without destructive impact upon infrastructure or fatalities and this region wasn’t yet the object of avalanche research. The existing ski infrastructure of Parâng resort located in the west of Parâng Mountains is proposed to be extended in the steep slopes of subalpine area. Field evidence pinpoints that these steep slopes were affected by snow avalanches in the past. In this study we analyzed 11 stem discs and 31 increment cores extracted from 22 spruces (Picea abies (L. Karst impacted by avalanches, in order to obtain more information about past avalanches activity. Using the dendrogeomorphological approach we found 13 avalanche events that occurred along Scărița avalanche path, since 1935 until 2012, nine of them produced in the last 20 years. The tree-rings data inferred an intense snow avalanche activity along this avalanche path. This study not only calls for more research in the study area but also proves that snow avalanches could constitute an important restrictive factor for the tourism infrastructure and related activities in the area. It must be taken into consideration by the future extension of tourism infrastructure. Keywords: snow avalanche, Parâng Mountains, dendrogeomorphology, ski area.

  17. Sammenhæng mellem luftvejssygdomme i den tidlige barndom og astma hos unge. International forskning, udvalgt og kommenteret

    DEFF Research Database (Denmark)

    Pedersen, P.A.

    1994-01-01

    Referat af udenlandsk arbejde vedr. sammenhæng mellem luftvejssygdomme i tidlig barndom og senere astma. Udgivelsesdato: 1994......Referat af udenlandsk arbejde vedr. sammenhæng mellem luftvejssygdomme i tidlig barndom og senere astma. Udgivelsesdato: 1994...

  18. Is extended biopsy protocol justified in all patients with PSA ≥ 20 ng/mL?

    Institute of Scientific and Technical Information of China (English)

    Xiaojun Deng; Jian Chu; Bo Yang; Feng Liu; Weifeng Wang; Jidong Hao; Jiansheng Wan; Hui Liu

    2014-01-01

    Objective: The aim of this study was to investigate whether it was necessary to increase the number of cores at initial prostate biopsy with patients of prostate-specific antigen (PSA) ≥ 20 ng/mL and to explore an appropriate individual-ized transrectal ultrasonograhpy (TRUS)-guided prostate biopsy for the detection of prostate cancer in men suspicious of prostate cancer.Methods:A total of 115 patients with PSA ≥ 20 ng/mL and suspicious of prostate cancer were prospectively randomized to perform TRUS-guided biopsy. Patients were randomized to a “6 + X” cores or a “10 + X” cores protocol. The primary end point was cancer detection rate. Secondary end points were cancer characteristics, rate of complications and the level of pain experienced by patients during TRUS-guided prostate biopsy.Results:Preoperative variables were similar in both groups. The overal prostate cancer detection rate was 73.9%. The “10 + X” cores strategy increased cancer detection rate only 9.7% in patients with PSA ≥ 20 ng/mL but < 50 ng/mL, while there was no diference between the two strategies for cancer detection in patients with PSA ≥ 50.1 ng/mL. The number of extended biopsy cores and pain score of extended biopsy in prostate cancer patients increased significantly (P < 0.001).Conclusion:Our findings suggest that there is no significant advantage in using extended biopsy protocol in al patients with PSA ≥ 20 ng/mL.

  19. Hans Küng se voorstel vir ’n eietydse etiek

    Directory of Open Access Journals (Sweden)

    S. A. Strauss

    1998-06-01

    Full Text Available This contribution undertakes a critical assessment of the well-known Hans Küng's proposal for contemporary ethics in his book Global responsibility. In search of a new world ethic (1990. This article commences by providing some background on this publication as well as on the theology of Küng in general. The content of the book is then paraphrased in summarised form. The last part of the article analyses four major convictions of Kung, against which the Reformed doctrine is brought forward. These convictions deal with the ethical aspects of normativity, humanity, postmodernity and ecumenicity.

  20. The curious case of NG2 cells: transient trend or game changer?

    Directory of Open Access Journals (Sweden)

    Vittorio Gallo

    2011-03-01

    Full Text Available It has been 10 years since the seminal work of Dwight Bergles and collaborators demonstrated that NG2 (nerve/glial antigen 2-expressing oligodendrocyte progenitor cells (NG2 cells receive functional glutamatergic synapses from neurons (Bergles et al., 2000, contradicting the old dogma that only neurons possess the complex and specialized molecular machinery necessary to receive synapses. While this surprising discovery may have been initially shunned as a novelty item of undefined functional significance, the study of neuron-to-NG2 cell neurotransmission has since become a very active and exciting field of research. Many laboratories have now confirmed and extended the initial discovery, showing for example that NG2 cells can also receive inhibitory GABAergic synapses (Lin and Bergles, 2004 or that neuron-to-NG2 cell synaptic transmission is a rather ubiquitous phenomenon that has been observed in all brain areas explored so far, including white matter tracts (Kukley et al., 2007; Ziskin et al., 2007; Etxeberria et al., 2010. Thus, while still being in its infancy, this field of research has already brought many surprising and interesting discoveries, and has become part of a continuously growing effort in neuroscience to re-evaluate the long underestimated role of glial cells in brain function (Barres, 2008. However, this area of research is now reaching an important milestone and its long-term significance will be defined by its ability to uncover the still elusive function of NG2 cells and their synapses in the brain, rather than by its sensational but transient successes at upsetting the old order established by neuronal physiology. To participate in the effort to facilitate such a transition, here we propose a critical review of the latest findings in the field of NG2 cell physiology – discussing how they inform us on the possible function(s of NG2 cells in the brain – and we present some personal views on new directions the field could

  1. "Klaaspärlimäng" esitleb "kiiksuga" klassikat / Karin Klaus

    Index Scriptorium Estoniae

    Klaus, Karin

    2007-01-01

    Muusikafestivalist "Klaaspärlimäng" (korraldaja Peeter Vähi). Erkki-Sven Tüüri heliplaadi "Oxymoron" esitlusest festivali avakontserdil "Aga kõrv ei puhka..." 6. juulil Pärnu kontserdimajas. Kontsertide kava 6. juulist 9. augustini

  2. Jenna Ng (ed., Understanding Machinima. Essays on Filmmaking in Virtual Worlds

    Directory of Open Access Journals (Sweden)

    Vincenzo Idone Cassone

    2015-09-01

    Full Text Available Understanding Machinima. Essays on Filmmaking in Virtual Worlds (Bloomsbury 2013, edited by Jenna Ng is an intriguing incursion into the world of machinima from a Media Studies point-of-view, trying to address the topic beyond game’s environment, in connection with the evolution of the contemporary mediascape.

  3. 76 FR 72087 - Special Conditions: Diamond Aircraft Industries, Model DA-40NG; Electronic Engine Control (EEC...

    Science.gov (United States)

    2011-11-22

    .... A47CE to include the new model DA- 40NG with the Austro Engine GmbH model E4 Aircraft Diesel Engine (ADE... the effects of the aircraft supplied power and data failures on the engine control system, and the... Engine GmbH model E4 aircraft diesel engine. 1. Electronic Engine Control a. For electronic...

  4. Lavale jõuab näitemäng armastuse orjast

    Index Scriptorium Estoniae

    2007-01-01

    Komöödiateatril tuleb lavale Jaan Toominga lavastatud "Venuse armumäng" austria kirjaniku Leopold von Sacher-Masochi romaani "Venus karusnahas" järgi. Osades Veikko Täär, Britta Vahur ja kulturist Imre Vähi

  5. Puuduv lugu, ainult kangelane : Zidane ja kunsti mäng / Zoe Stillpass

    Index Scriptorium Estoniae

    Stillpass, Zoe

    2010-01-01

    Režissööride Philippe Parreno ja Douglas Gordoni film prantsuse jalgpallurist Zinedine Zidanest. Film on 23. aprillil 2005 peetud mäng Real Madridi ja Villareali vahel Madridis, mida filmiti 17 kaameraga, mis kõik olid fokusseeritud Zidane'ile. Helitaust pärineb šoti alternatiivroki bändilt Mogwai

  6. Genetic polymorphism and forensic parameters of nine short tandem repeat loci in Ngöbé and Emberá Amerindians of Panama.

    Science.gov (United States)

    Castro, Edgardo A; Trejos, Diomedes E; Berovides-Alvarez, Vicente; Arias, Tomás D; Ramos, Carlos W

    2007-10-01

    Nine STR loci (CSF1PO, TPOX, TH01, F13A01, FESFPS, VWA, D16S539, D7S820, and D13S317) were analyzed in unrelated Ngöbé and Emberá Amerindians of Panama. The chi-square test demonstrated statistically significant differences (P shared their alleles with the highest frequencies in seven loci. However, there were also noticeable differences at the TPOX locus, which showed its highest frequencies at alleles 11 (0.48) and 6 (0.54) for the Ngöé and Emberá, respectively. Interestingly, these alleles are present in one population and are absent in the other, suggesting that they could be distinctive for each population. These results demonstrate that, despite the fact that each population belongs to a different linguistic stock [Chibchan (Ngöbé) and Chocoan (Emberá)], both retain strong similarities in their allele-frequency distributions. Three loci (TPOX, VWA, and F13A01) in the Ngöbé and two loci (TH01 and TPOX) in the Emberá departed from Hardy-Weinberg equilibrium. The analysis of the STR markers demonstrates that, despite their low levels of genetic polymorphisms, most of them could be informative for forensic purposes, showing a combined power of discrimination of 0.9999 for both Amerindian populations. However, powers of exclusion in the Ngöbé were very low, particularly at the TH01 (0.04) and FESFPS (0.08) loci. The combined powers of exclusion were 0.9338 and 0.9890 for the Ngöbé and the Emberá, respectively. Furthermore, the combined typical paternity index in the Ngöbé was considerably low (2.58), and in the Emberá it was 40.44, which is also very low. The low genetic polymorphism levels suggest that theuse of additional loci supplementing the battery of the nine loci is recommended for paternity and forensic tests in both populations, particularly for the Ngöbé.

  7. Multi-analyte approach for the determination of ng L(-1) levels of steroid hormones in unidentified aqueous samples.

    Science.gov (United States)

    Noppe, H; Verheyden, K; Gillis, W; Courtheyn, D; Vanthemsche, P; De Brabander, H F

    2007-03-14

    Since the 1970s, many analytical methods for the detection of illegal growth promoters, such as thyreostats, anabolics, beta-agonists and corticosteroids have been developed for a wide range of matrices of animal origin, including meat, fat, organ tissue, urine and faeces. The aim of this study was to develop an analytical method for the determination of ng L(-1) levels of estrogens, gestagens, androgens (EGAs) and corticosteroids in aqueous preparations (i.e. drinking water, drinking water supplements), commercially available on the 'black' market. For this, extraction was performed with Bakerbond C18 speedisk, a technique commonly used in environmental analysis. After fractionation, four fractions were collected using a methanol:water gradient program. Gas chromatography coupled to electron impact multiple mass spectrometry (GC-EI-MS2) screening for the EGAs was carried out on the derivatized extracts. For the detection of corticosteroids, gas chromatography coupled to negative chemical ionization mass spectrometry (GC-NCI-MS) was used after oxidation of the extracts. Confirmation was done by liquid chromatography coupled to electrospray ionization multiple mass spectrometry (LC-ESI-MS2). The combined use of GC and LC coupled to MS enabled the identification and quantification of anabolics and corticosteroids at the low ng L(-1) level. This study demonstrated the occurrence of both androgens and corticosteroids in different commercial aqueous samples.

  8. Multi-analyte approach for the determination of ng L-1 levels of steroid hormones in unidentified aqueous samples

    International Nuclear Information System (INIS)

    Since the 1970s, many analytical methods for the detection of illegal growth promoters, such as thyreostats, anabolics, β-agonists and corticosteroids have been developed for a wide range of matrices of animal origin, including meat, fat, organ tissue, urine and faeces. The aim of this study was to develop an analytical method for the determination of ng L-1 levels of estrogens, gestagens, androgens (EGAs) and corticosteroids in aqueous preparations (i.e. drinking water, drinking water supplements), commercially available on the 'black' market. For this, extraction was performed with Bakerbond C18 speedisk, a technique commonly used in environmental analysis. After fractionation, four fractions were collected using a methanol:water gradient program. Gas chromatography coupled to electron impact multiple mass spectrometry (GC-EI-MS2) screening for the EGAs was carried out on the derivatized extracts. For the detection of corticosteroids, gas chromatography coupled to negative chemical ionization mass spectrometry (GC-NCI-MS) was used after oxidation of the extracts. Confirmation was done by liquid chromatography coupled to electrospray ionization multiple mass spectrometry (LC-ESI-MS2). The combined use of GC and LC coupled to MS enabled the identification and quantification of anabolics and corticosteroids at the low ng L-1 level. This study demonstrated the occurrence of both androgens and corticosteroids in different commercial aqueous samples

  9. Research of stress corrosion cracking of T225NG titanium alloy in loop water of high temperature and high pressure

    Institute of Scientific and Technical Information of China (English)

    Xu Jijin; Yan Keng; Chen Ligong; Jiang Chengyu

    2006-01-01

    Double cantilever beam (DCB) specimens were used to research the stress corrosion cracking of T225NG titanium alloy in loop water of high temperature and high pressure. DCB specimens were forced pre-stress, put into high pressure autoclave, and the stress corrosion and crack expansion of specimens were observed and measured in 500 h, 1 000 h and 2 000h respectively. The results show that small expansion occurred along the direction of pre-cracking. According to calculation,the speed of cracking expansion is lower than 10 -9 m/s in 500 h and the value of KIscc/KI is higher than 0. 75, which proves that T225NG has an excellent corrosion resistance in loop water. The main reason is that there is an oxide film on the surface of specimens. According to the analysis of energy dispersive spectroscopy (EDS) and X-ray diffraction (XRD), the oxide film consists of TiO2. Therefore, the oxide film at the crack tip impedes the hydrogen separating out from the cathode to penetrate into titanium alloy and resists hydrogen embrittlement.

  10. Multi-analyte approach for the determination of ng L(-1) levels of steroid hormones in unidentified aqueous samples.

    Science.gov (United States)

    Noppe, H; Verheyden, K; Gillis, W; Courtheyn, D; Vanthemsche, P; De Brabander, H F

    2007-03-14

    Since the 1970s, many analytical methods for the detection of illegal growth promoters, such as thyreostats, anabolics, beta-agonists and corticosteroids have been developed for a wide range of matrices of animal origin, including meat, fat, organ tissue, urine and faeces. The aim of this study was to develop an analytical method for the determination of ng L(-1) levels of estrogens, gestagens, androgens (EGAs) and corticosteroids in aqueous preparations (i.e. drinking water, drinking water supplements), commercially available on the 'black' market. For this, extraction was performed with Bakerbond C18 speedisk, a technique commonly used in environmental analysis. After fractionation, four fractions were collected using a methanol:water gradient program. Gas chromatography coupled to electron impact multiple mass spectrometry (GC-EI-MS2) screening for the EGAs was carried out on the derivatized extracts. For the detection of corticosteroids, gas chromatography coupled to negative chemical ionization mass spectrometry (GC-NCI-MS) was used after oxidation of the extracts. Confirmation was done by liquid chromatography coupled to electrospray ionization multiple mass spectrometry (LC-ESI-MS2). The combined use of GC and LC coupled to MS enabled the identification and quantification of anabolics and corticosteroids at the low ng L(-1) level. This study demonstrated the occurrence of both androgens and corticosteroids in different commercial aqueous samples. PMID:17386692

  11. Tiềm năng lớn của vệ tinh siêu nhỏ đáp ứng nhu cầuquản lý hàng hải của Việt Nam

    OpenAIRE

    Vu Trong, Thu; Pham Quang, Hung; Nguyen Hoang, Giang; Nguyen, Hugo

    2011-01-01

    Trong 10 năm gần đây, loại vệ tinh siêu nhỏ có kích thước 10x10x10 cm và trọng lượng 1 kg với tên gọi CubeSat từ công cụ thực hành của sinh viên đại học đang dần trở thành nền tảng cho những vệ tinh có khả năngng dụng trong thực tiễn. Ngày nay, người sử dụng có thể tự thiết kế hoặc mua sẵn linh kiện điện tử và cơ khí lắp thành vệ tinh cỡ 1, 2, 3... kg với các tính năng mong muốn. Ưu điểm của loại vệ tinh siêu nhỏ này là thời gian chế tạo ngắn, chi phí thấp và càng phát huy năng lực khi sử d...

  12. Návrh zatahovacího podvozku pro letoun NG4

    OpenAIRE

    BARTONĚK, Jaroslav

    2011-01-01

    Diplomová práce popisuje návrh způsobu zatahování podvozku pro ultralehký letoun NG-4. Jejími hlavními částmi jsou popis všeobecného principu zatahování, návrh kinematiky, výpočet zatížení a pevnostní kontrola, volba potřebných materiálů a způsob ovládání podvozku pilotem. Okrajovou náplní práce je členění letadlových podvozků a jejich historický vývoj. This diploma thesis describes the design of retractable landing gear for the NG-4 ultralight aircraft. Its main part consists of general p...

  13. NEW ASPECTS OF THE ALPINE VEGETATION OF PARÂNG MOUNTAINS (SOUTH CARPATHIANS

    Directory of Open Access Journals (Sweden)

    SIMON TIBOR

    2012-12-01

    Full Text Available 4 plant communities unknown in the the European syntaxonomy are described from the alpine and subalpine belts of Parâng Mountains, based on vegetation studies of the authors during 1955–1960. These are: Arabis alpina-Saxifraga aizoides, Arabis alpina-Delphinium elatum, Dianthus tenuifolius-Festuca dalmatica and the Primula minima-Dryas octopetala communities. These communities could be described later as new associations in the possession of more relevés from different localities. The East and South Carpathian Doronico carpatici-Festucetum pictae association is distinguished under this new name from the Festucetum pictae Krajina described from the Tatra Mountains. 7 further associations are found as new to the Parâng Mountains.

  14. The Available Options for Wavelength Group Selection and Transceiver Design for Next Generation PON Stage 2 (NG-PON2)

    Science.gov (United States)

    Niazi, Shahab Ahmad; Zhang, Xiaoguang; Xi, Lixia; Chen, Yong Li; Idress, Muhammad

    2013-09-01

    The full service access network (FSAN) task group meeting was held in April 2012, where they agreed to adopt time and wavelength division multiplexed passive optical network (TWDM-PON) as a primary solution to NG-PON2. Major requirements includes at least 40 Gbit/s aggregate rate in downstream, 1:64 split ratio, 40 km differential reach, and at least 1 Gb/s access rate per optical network unit (ONU). In this paper, a performance analysis will be carried out for downstream 4-wavelengths group selection from S-band, L-band, and C-band. A comparison for optimal transmitter design is also investigated among probable options of chirp managed laser diode, Lithium Niobate (LiNbo3) Mach Zehnder modulator, besides examining tunable transceivers in contrary to remotely fed colorless ONUs.

  15. LA FRASE NOMINAL NGÄBÉRE The Nominal Phrase Ngäbére

    Directory of Open Access Journals (Sweden)

    José Manuel Murillo Miranda

    2009-12-01

    Full Text Available Este trabajo es el avance de una amplia investigación cuyo propósito es elaborar una gramática descriptiva de la lengua chibcha ngäbére. El artículo presenta las características particulares más relevantes de la frase nominal de dicha variante dialectal (hablada en Costa Rica, también en Panamá, y establece la relación con algunas de las características generales de otras lenguas chibchas ístmicas. El primer apartado del escrito es una introducción a la lengua ngäbére y a sus hablantes; el segundo presenta la morfología de los sustantivos, pronombres y otras clases de palabras que conforman la frase nominal; el tercer apartado trata de la sintaxis de la frase nominal y esboza, de manera sucinta, las relaciones gramaticales; el cuarto apartado es una conclusión valorativa de este trabajo.This work is a preview of a broader study whose goal is to create a descriptive grammar of the Chibchan language, Ngäbere. The article describes the most relevant features of the dialect's nominal phrase (spoken in Costa Rica and Panama and establishes the relationship with some of the general features of other Isthmian Chibchan languages. The first part of the report is an introduction to Ngäbere and it speakers; the second, describes the morphology of the nouns, pronouns, and other words that make up the nominal phrase; the third, is about the syntax of the nominal phrase and it outlines in a succinct manner, grammatical relationships; the last part is a critical conclusion of this work.

  16. Phenotype overlap in glial cell populations: astroglia, oligodendroglia and NG-2(+ cells

    Directory of Open Access Journals (Sweden)

    Robert eFern

    2015-05-01

    Full Text Available The extent to which NG-2(+ cells form a distinct population separate from astrocytes is central to understanding whether this important cell class is wholly an oligodendrocyte precursor cell (OPC or has additional functions akin to those classically ascribed to astrocytes. Early immuno-staining studies indicate that NG-2(+ cells do not express the astrocyte marker GFAP, but orthogonal reconstructions of double-labelled confocal image stacks here reveal a significant degree of co-expression in individual cells within post-natal day 10 (P10 rat optic nerve (RON and rat cortex. Extensive scanning of various antibody/fixation/embedding approaches identified a protocol for selective post-embedded immuno-gold labelling. This first ultrastructural characterization of identified NG-2(+ cells revealed populations of both OPCs and astrocytes in P10 RON. NG-2(+ astrocytes had classic features including the presence of glial filaments but low levels of glial filament expression were also found in OPCs and myelinating oligodendrocytes. P0 RONs contained few OPCs but positively identified astrocytes were observed to ensheath pre-myelinated axons in a fashion previously described as a definitive marker of the oligodendrocyte lineage. Astrocyte ensheathment was also apparent in P10 RONs, was absent from developing nodes of Ranvier and was never associated with compact myelin. Astrocyte processes were also shown to encapsulate some oligodendrocyte somata. The data indicate that common criteria for delineating astrocytes and oligodendroglia are insufficiently robust and that astrocyte features ascribed to OPCs are likely to arise from misidentification.

  17. Localisation of NG2 immunoreactive neuroglia cells in the rat locus coeruleus and their plasticity in response to stress

    Directory of Open Access Journals (Sweden)

    Mohsen eSeifi

    2014-05-01

    Full Text Available The locus coeruleus (LC nucleus modulates adaptive behavioural responses to stress and dysregulation of LC neuronal activity is implicated in stress-induced mental illnesses. The LC is composed primarily of noradrenergic neurons together with various glial populations. A neuroglia cell-type largely unexplored within the LC is the NG2 cell. NG2 cells serve primarily as oligodendrocyte precursor cells throughout the brain. However, some NG2 cells are in synaptic contact with neurons suggesting a role in information processing. The aim of this study was to neurochemically and anatomically characterise NG2 cells within the rat LC. Furthermore, since NG2 cells have been shown to proliferate in response to traumatic brain injury, we investigated whether such NG2 cells plasticity also occurs in response to emotive insults such as stress. Immunohistochemistry and confocal microscopy revealed that NG2 cells were enriched within the pontine region occupied by the LC. Close inspection revealed that a sub-population of NG2 cells were located within unique indentations of LC noradrenergic somata and were immunoreactive for the neuronal marker NeuN whilst NG2 cell processes formed close appositions with clusters immunoreactive for the inhibitory synaptic marker proteins gephyrin and the GABA-A receptor alpha3-subunit, on noradrenergic dendrites. In addition, LC NG2 cell processes were decorated with vesicular glutamate transporter 2 immunoreactive puncta. Finally, ten days of repeated restraint stress significantly increased the density of NG2 cells within the LC. The study demonstrates that NG2 IR cells are integral components of the LC cellular network and they exhibit plasticity as a result of emotive challenges.

  18. The NG2 Proteoglycan Protects Oligodendrocyte Precursor Cells against Oxidative Stress via Interaction with OMI/HtrA2.

    Directory of Open Access Journals (Sweden)

    Frank Maus

    Full Text Available The NG2 proteoglycan is characteristically expressed by oligodendrocyte progenitor cells (OPC and also by aggressive brain tumours highly resistant to chemo- and radiation therapy. Oligodendrocyte-lineage cells are particularly sensitive to stress resulting in cell death in white matter after hypoxic or ischemic insults of premature infants and destruction of OPC in some types of Multiple Sclerosis lesions. Here we show that the NG2 proteoglycan binds OMI/HtrA2, a mitochondrial serine protease which is released from damaged mitochondria into the cytosol in response to stress. In the cytosol, OMI/HtrA2 initiates apoptosis by proteolytic degradation of anti-apoptotic factors. OPC in which NG2 has been downregulated by siRNA, or OPC from the NG2-knockout mouse show an increased sensitivity to oxidative stress evidenced by increased cell death. The proapoptotic protease activity of OMI/HtrA2 in the cytosol can be reduced by the interaction with NG2. Human glioma expressing high levels of NG2 are less sensitive to oxidative stress than those with lower NG2 expression and reducing NG2 expression by siRNA increases cell death in response to oxidative stress. Binding of NG2 to OMI/HtrA2 may thus help protect cells against oxidative stress-induced cell death. This interaction is likely to contribute to the high chemo- and radioresistance of glioma.

  19. Characterization of cells recovered from the xenotransplanted NG97 human-derived glioma cell line subcultured in a long-term in vitro

    Directory of Open Access Journals (Sweden)

    Heinrich Juliana K

    2008-10-01

    Full Text Available Abstract Background In order to elucidate tumoral progression and drug resistance, cultured cell lines are valuable tools applied on tumor related assays provided they are well established and characterized. Our laboratory settled the NG97 cell line derived from a human astrocytoma grade III, which started to develop and express important phenotypical characteristics of an astrocytoma grade IV after injection in the flank of nude mice. Astrocytomas are extremely aggressive malignancies of the Central Nervous System (CNS and account for 46% of all primary malignant brain tumors. Progression to worse prognosis occurs in 85% of the cases possibly due to changes in cell tumor microenvironment and through biological pathways that are still unclear. Methods This work focused on characterizing the NG97 cell line specifically after being recovered from the xenotransplant, who maintained their undifferentiated characteristics along the following 60th passages in vitro. These cells were subcultivated to evaluate the possible contribution of these undifferentiated characteristics to the malignant progression phenotype. These characteristics were the expression of molecules involved in the processes of migration, dedifferentiation and chromosomal instability. Results Results showed that NG97(ht had an decrease in doubling time through sub cultivation, which was characterized by a converse modulation between the expression of glial fibrillary acidic protein (GFAP and vimentin. In addition, β1 integrins were present in intermediate levels while α5 integrins had a high expression profile as well as fibronectin and laminin. Cytogenetic analysis of NG97(ht revealed several chromosomal abnormalities, 89% of the cells showed to be hyperdiploid and the modal number was assigned to be 63. Several acrocentric chromosomes were visualized and at least 30 figures were attributed to be murine. These findings suggest a possible fusion between the original NG97 cells

  20. Straightforward strategy for quantifying rotundone in wine at ngL(-1) level using solid-phase extraction and gas chromatography-quadrupole mass spectrometry. Occurrence in different varieties of spicy wines.

    Science.gov (United States)

    Culleré, Laura; Ontañón, Ignacio; Escudero, Ana; Ferreira, Vicente

    2016-09-01

    This paper presents a straightforward methodology to quantify rotundone in wine at ngL(-1) level. This compound, responsible for the black pepper aromatic note, may have sensorial relevance in some wines due to its low odor threshold, estimated at only 16ngL(-1) in red wines. The proposed strategy is based on solid phase extraction and analysis by GC-MS. The detection limit value was 0.6ngL(-1), which is more than one order of magnitude below its odor threshold in wine. Matrix effects have not been found and a synthetic wine calibration was proposed. The precision of the method was evaluated in reproducibility terms, obtaining a very acceptable value (RSD 4%). The optimized and validated strategy was applied to quantify this molecule in thirty wines belonging to different varieties as Graciano, Maturana tinta, Schioppettino, Shiraz, Duras and Gamay. Two of these wines exhibited levels higher than 100ngL(-1).

  1. Straightforward strategy for quantifying rotundone in wine at ngL(-1) level using solid-phase extraction and gas chromatography-quadrupole mass spectrometry. Occurrence in different varieties of spicy wines.

    Science.gov (United States)

    Culleré, Laura; Ontañón, Ignacio; Escudero, Ana; Ferreira, Vicente

    2016-09-01

    This paper presents a straightforward methodology to quantify rotundone in wine at ngL(-1) level. This compound, responsible for the black pepper aromatic note, may have sensorial relevance in some wines due to its low odor threshold, estimated at only 16ngL(-1) in red wines. The proposed strategy is based on solid phase extraction and analysis by GC-MS. The detection limit value was 0.6ngL(-1), which is more than one order of magnitude below its odor threshold in wine. Matrix effects have not been found and a synthetic wine calibration was proposed. The precision of the method was evaluated in reproducibility terms, obtaining a very acceptable value (RSD 4%). The optimized and validated strategy was applied to quantify this molecule in thirty wines belonging to different varieties as Graciano, Maturana tinta, Schioppettino, Shiraz, Duras and Gamay. Two of these wines exhibited levels higher than 100ngL(-1). PMID:27041325

  2. Effects of NG-nitro-L-arginine on focal cerebral ischemic injury in rats

    Institute of Scientific and Technical Information of China (English)

    Jianxin Zhang; Huixin Zhang; Lanfang Li; Yonghui Li

    2006-01-01

    BACKGROUND: Previous researches have proved that aminoguanidin can cure cerebral ischemic injury remarkably as a selective induced nitricoxide synthase (iNOS) inhibitor. However, whether nonselective NOS inhibitor could protect cerebral ischemic injury or not is unclear.OBJECTIVE: To investigate the effects of NG-nitro-L-arginine (L-NA), a nonselective nitricoxide synthase (NOS) inhibitor, on cerebral ischemic injury of rats and the possible mechanism.DESIGN: Randomized controlled study.SETTING: Pharmacological Department of Medical Academy of Science of Hebei Province.MATERIALS: A total of 56 male healthy SD rats, of grade II, weighting 250-290 g, were provided by the Experimental Animal Center of Hebei Province (certification: 04036).METHODS: The experiment was completed in the Pharmacological Department of Medical Academy of Science of Hebei Province from March 2005 to January 2006. ① Grouping: Rats were randomly divided into 3 groups: sham operation group (n=8), model group (n=24) and L-NA group (n=24). ② Modeling:Middle cerebral artery (MCA) was established on rats in model group and L-NA group with intraluminal line occlusion methods, and rats in sham operation group were separated their external carotid arteries without occlusion of internal carotid artery. ③ Intervention study: Rats in model group and L-NA group were injected intraperitoneally with 10 mL/kg and 20 mg/kg L-NA at 2, 6 and 12 hours respectively after ischemia twice a day for 3 consecutive days. ④ Rats were sacrificed on the third day for measuring volume of cerebral infarction with image analysis and swelling degrees and activities of mitochondria with electron microscope. Effect of L-NA on ultrastructural changes of neurons in cortex was observed after ischemia.MAIN OUTCOME MEASURES: ① Volume of cerebral infarction; ② Swelling degrees, contents of nitric oxide (NO) and malondialdehyde (MDA) and activities of adenosine triphosphatase (ATPase), superoxide dismutase (SOD) and

  3. APOLLO_NG – a probabilistic interpretation of the APOLLO legacy for AVHRR heritage channels

    Directory of Open Access Journals (Sweden)

    L. Klüser

    2015-04-01

    Full Text Available The cloud processing scheme APOLLO (Avhrr Processing scheme Over cLouds, Land and Ocean has been in use for cloud detection and cloud property retrieval since the late 1980s. The physics of the APOLLO scheme still build the backbone of a range of cloud detection algorithms for AVHRR (Advanced Very High Resolution Radiometer heritage instruments. The APOLLO_NG (APOLLO_NextGeneration cloud processing scheme is a probabilistic interpretation of the original APOLLO method. While building upon the physical principles having served well in the original APOLLO a couple of additional variables have been introduced in APOLLO_NG. Cloud detection is not performed as a binary yes/no decision based on these physical principals but is expressed as cloud probability for each satellite pixel. Consequently the outcome of the algorithm can be tuned from clear confident to cloud confident depending on the purpose. The probabilistic approach allows to retrieving not only the cloud properties (optical depth, effective radius, cloud top temperature and cloud water path but also their uncertainties. APOLLO_NG is designed as a standalone cloud retrieval method robust enough for operational near-realtime use and for the application with large amounts of historical satellite data. Thus the radiative transfer solution is approximated by the same two stream approach which also had been used for the original APOLLO. This allows the algorithm to be robust enough for being applied to a wide range of sensors without the necessity of sensor-specific tuning. Moreover it allows for online calculation of the radiative transfer (i.e. within the retrieval algorithm giving rise to a detailed probabilistic treatment of cloud variables. This study presents the algorithm for cloud detection and cloud property retrieval together with the physical principles from the APOLLO legacy it is based on. Furthermore a couple of example results from on NOAA-18 are presented.

  4. APOLLO_NG - a probabilistic interpretation of the APOLLO legacy for AVHRR heritage channels

    Science.gov (United States)

    Klüser, L.; Killius, N.; Gesell, G.

    2015-10-01

    The cloud processing scheme APOLLO (AVHRR Processing scheme Over cLouds, Land and Ocean) has been in use for cloud detection and cloud property retrieval since the late 1980s. The physics of the APOLLO scheme still build the backbone of a range of cloud detection algorithms for AVHRR (Advanced Very High Resolution Radiometer) heritage instruments. The APOLLO_NG (APOLLO_NextGeneration) cloud processing scheme is a probabilistic interpretation of the original APOLLO method. It builds upon the physical principles that have served well in the original APOLLO scheme. Nevertheless, a couple of additional variables have been introduced in APOLLO_NG. Cloud detection is no longer performed as a binary yes/no decision based on these physical principles. It is rather expressed as cloud probability for each satellite pixel. Consequently, the outcome of the algorithm can be tuned from being sure to reliably identify clear pixels to conditions of reliably identifying definitely cloudy pixels, depending on the purpose. The probabilistic approach allows retrieving not only the cloud properties (optical depth, effective radius, cloud top temperature and cloud water path) but also their uncertainties. APOLLO_NG is designed as a standalone cloud retrieval method robust enough for operational near-realtime use and for application to large amounts of historical satellite data. The radiative transfer solution is approximated by the same two-stream approach which also had been used for the original APOLLO. This allows the algorithm to be applied to a wide range of sensors without the necessity of sensor-specific tuning. Moreover it allows for online calculation of the radiative transfer (i.e., within the retrieval algorithm) giving rise to a detailed probabilistic treatment of cloud variables. This study presents the algorithm for cloud detection and cloud property retrieval together with the physical principles from the APOLLO legacy it is based on. Furthermore a couple of example

  5. Implementation of Order-wire Functionality in IRITEL NG-SDH devices

    Directory of Open Access Journals (Sweden)

    D. Katanić

    2011-06-01

    Full Text Available This paper describes the implementation of order-wire functionality in NG-SDH devices ODS2G5 and OTS622 IRITEL. The paper also describes the operation of order-wire in networks with arbitrary topologies, as well as the configuration of order-wire functionality by using network management software SUNCE. The presented solution for order-wire functionality is compared with VoIP solutions. The paper shows that the presented solution provides fast reaction to network failures, while keeping the implementation simple.

  6. Aurora candidates from the chronicle of Qíng dynasty in several degrees of relevance

    Science.gov (United States)

    Kawamura, Akito D.; Hayakawa, Hisashi; Tamazawa, Harufumi; Miyahara, Hiroko; Isobe, Hiroaki

    2016-08-01

    We present the result of a survey of sunspots and auroras in Qíngshǐgǎo (清史稿), a draft chronicle of Qíng dynasty, for the period of 1559-1912 CE. This is a sequel to a series of works surveying historical sunspot and aurora records, and providing online data to the scientific community regarding the attained results. In total of this Qíngshǐgǎo survey, we found 111 records of night-sky luminous events with such keywords as vapor (氣, qì), cloud (雲, yún), and light (光, guāng), which may indicate auroras as well as some other phenomena. Similarly, a keyword survey for sunspots was conducted, but no sunspot record was found. In comparison with the aurora records in the western world, we found that 14 of the 111 records have a corresponding record of simultaneous observation in the western world, and hence are very likely to be aurora. In order to investigate the likeliness of the remainder of the record being aurora, we calculated the lunar age and the phase of a solar cycle for each record. After these calculations, a notable fraction of these records clustered near the full moon were to be found statistically doubtful in considerations with atmospheric optics; meanwhile, a few records of observations near the new moon could be more likely interpreted as being auroras, including three records during the Maunder minimum.

  7. Radical prostatectomy outcome when performed with PSA above 20 ng/ml.

    LENUS (Irish Health Repository)

    Connolly, S S

    2012-02-01

    Many centres currently do not offer radical prostatectomy (RP) to men with high-risk localised prostate cancer due to concerns regarding poor outcome, despite evidence to the contrary. We identified 18 men undergoing RP with serum PSA >20 ng\\/ml (high-risk by National Comprehensive Cancer Network definition) and minimum follow-up of 12 years (mean 13.5). Mean preoperative PSA was 37.0 ng\\/ml (Range 21.1-94.0). Prostatectomy pathology reported extracapsular disease in 16 (88.9%), positive surgical margins in 15 (83%) and positive pelvic lymph nodes in 5 (27.8%). Overall and cancer-specific survival at 5 and 10-years was 83.3%, 88.2%, 72% and 76.5% respectively. With complete follow-up 11 (61.1%) are alive, and 5 (27.8%) avoided any adjuvant therapy. Complete continence (defined as no involuntary urine leakage and no use of pads) was achieved in 60%, with partial continence in the remainder. We conclude that surgery for this aggressive variant of localised prostate cancer can result in satisfactory outcome.

  8. NC/NG与ADN的相互作用%Interaction of NG/NC with ADN

    Institute of Scientific and Technical Information of China (English)

    张腊莹; 衡淑云; 刘子如; 张皋; 赵凤起; 谭惠民

    2009-01-01

    用高压差示扫描量热法(PDSC),热重-微商热重法(TG-DTG),研究了双基粘合剂(NC/NG体系)与二硝酰胺铵(ADN)之间的相互作用.结果表明,(NC/NG)/ADN体系中NG的分解峰由NC/NG中的207.0 ℃提前至159.8 ℃,在高压下该分解峰温提前至153.6 ℃.NC/ADN的分解峰温比NG/ADN提前了4.8 ℃,而且大部分的ADN没有被NG加速而提前分解,表明NC对ADN的作用比NG更强烈.此外,还用真空安定性试验(VST)评价了NC/NG与ADN的相容性,混合体系的净增放气量大于11 mL,属于严重不相容,证明在90 ℃下NC/NG与ADN之间也存在强烈的相互作用.

  9. Aurora Candidates from the Chronicle of Q\\={i}ng Dynasty in Several Degrees of Relevance

    CERN Document Server

    Kawamura, Akito D; Tamazawa, Harufumi; Miyahara, Hiroko; Isobe, Hiroaki

    2016-01-01

    We present the result of the survey of sunspots and auroras in ${\\it Q\\bar{\\imath}ngsh\\check{\\imath}ga\\check{o}}$, the draft chronicle of ${\\it Q\\bar{\\imath}ng}$ dynasty, for the period of 1559-1912 CE, as a sequel of the series of works surveying historical sunspot and aurora records, and providing online data to the scientific community regarding the attained results. In total of this ${\\it Q\\bar{\\imath}ngsh\\check{\\imath}ga\\check{o}}$ survey, we found 111 records of night-sky luminous events with the keywords such as vapor (${\\it q\\grave{\\imath}}$), cloud (${\\it y\\acute{u}n}$), and light (${\\it gu\\bar{a}ng}$), which may indicate auroras as well as some other phenomena. Similarly keyword survey for sunspots were done, but no sunspot record was found. In comparison with the aurora records in the western world, we found 14 of the 111 records have a corresponding record of simultaneous observation in the western world and hence are very likely to be aurora. In order to investigate the likeliness of the rest of ...

  10. The 6-hydroxydopamine-induced nigrostriatal neurodegeneration produces microglia-like NG2 glial cells in the rat substantia nigra.

    Science.gov (United States)

    Kitamura, Yoshihisa; Inden, Masatoshi; Minamino, Hideaki; Abe, Mari; Takata, Kazuyuki; Taniguchi, Takashi

    2010-11-01

    Neuron/glial 2 (NG2)-expressing cells are often referred to as oligodendrocyte precursor cells. NG2-expressing cells have also been identified as multipotent progenitor cells. However, microglia-like NG2 glial cells have not been fully examined in neurodegenerative disorders such as Parkinson's disease (PD). In the present study, we chose two rat models of PD, i.e., intranigral or intrastriatal injection of 6-hydroxydopamine (6-OHDA), since the cell bodies of dopamine (DA) neurons, which form a nigrostriatal pathway, are in the substantia nigra pars compacta (SNpc) while their nerve terminals are in the striatum. In the nigral 6-OHDA-injected model, activated NG2-positive cells were detected in the SNpc but not in the striatum. In contrast, in the striatal 6-OHDA-injected model, these cells were detected in both the SNpc and the striatum. In both models, activated NG2-positive cells were located close to surviving tyrosine hydroxylase (TH)-positive neurons in the SNpc. In addition, activated NG2-positive cells in the SNpc coexpressed ionized calcium-binding adaptor molecule 1 (Iba1), a microglia/macrophage marker. Interestingly, these double-positive glial cells coexpressed glial cell line-derived neurotrophic factor (GDNF). These results suggest that microglia-like NG2 glial cells may help protect DA neurons and may lead to new therapeutic targets in PD.

  11. NG2 Proteoglycan Promotes Endothelial Cell Motility and Angiogenesis via Engagement of Galectin-3 and α3β1 Integrin

    Science.gov (United States)

    Fukushi, Jun-ichi; Makagiansar, Irwan T.; Stallcup, William B.

    2004-01-01

    The NG2 proteoglycan is expressed by microvascular pericytes in newly formed blood vessels. We have used in vitro and in vivo models to investigate the role of NG2 in cross-talk between pericytes and endothelial cells (EC). Binding of soluble NG2 to the EC surface induces cell motility and multicellular network formation in vitro and stimulates corneal angiogenesis in vivo. Biochemical data demonstrate the involvement of both galectin-3 and α3β1 integrin in the EC response to NG2 and show that NG2, galectin-3, and α3β1 form a complex on the cell surface. Transmembrane signaling via α3β1 is responsible for EC motility and morphogenesis in this system. Galectin-3–dependent oligomerization may potentiate NG2-mediated activation of α3β1. In conjunction with recent studies demonstrating the early involvement of pericytes in angiogenesis, these data suggest that pericyte-derived NG2 is an important factor in promoting EC migration and morphogenesis during the early stages of neovascularization. PMID:15181153

  12. 硫酸软骨素蛋白多糖4细胞在成年大鼠中枢神经系统的定位分布及其异质性%Localization and heterogeneity of NG2 cells in central nervous system of adult rat*

    Institute of Scientific and Technical Information of China (English)

    唐文强; 孙善全; 朱淑娟; 龙志敏

    2011-01-01

    Objective: To observe the localization and heterogeneity of NG2 cells in the brain and spinal cord of adult rats.Methods: The localization and heterogeneity of NG2 cells in the cerebral cortex and spinal cord of adult rats was investigated by using immunohistochemical and immunofluorescence methods. The soma areas, the number of process and the length of process in NG2 cells were measured by image analysis program Image Pro Plus 6.0 and statistically analyzed by SPSS17.0.Results : NG2-positive cells were extensively distributed throughout the CNS of adult rats. The size of NG2 cells in the spinal cord was bigger than that in the cerebral cortex; The process length of NG2 cells in the spinal cord was shorter than that in the cerebral conex; The number of processes in NG2 cells in the cerebral cortex was bigger than that in the spinal cord.Conclusion: The results indicate that there are some morphological differences between NG2 cells in the spinal cord and NG2 cells in the cerebral cortex. It is suggested that NG2 cells would be heterogeneous in different regions of CNS.%目的:研究硫酸软骨素蛋白多糖4细胞(NG2细胞)在成年大鼠大脑与脊髓的定位分布及其异质性.方法:应用免疫组织化学、免疫荧光方法观察大脑与脊髓NG2阳性细胞分布及其形态特征.采用Image Pro Plus6.0图像分析软件对NG2阳性细胞胞体面积、突起个数、突起长度进行测量.应用SPSS17.0软件对结果进行统计学分析.结果:NG2细胞在成年大鼠中枢神经系统内广泛分布;在脑内NG2细胞突起数目较多,胞体较小;而在脊髓内NG2细胞突起较少,但其胞体却较大.结论:在脑和脊髓内NG2细胞的形态存在异质性,提示其在成年大鼠中枢神经系统两个部位具有不同的功能.

  13. Freed by trust, to believe together: Pursuing global ecumenism with Küng and Tracy

    Directory of Open Access Journals (Sweden)

    Jakub Urbaniak

    2014-04-01

    Full Text Available Focus and scope Publication ethics Journal metrics Frequent asked questions Supporting agencies Contact us Reading tools Print this article Indexing metadata Review policy Email this article Email the author Post a Comment Translate content Powered by Translate Related items Show all ✔ Open access ✔ Global visibility ✔ Rapid publication ✔ Quality peer review ✔ Author retains copyright ✔ Personalised service ✔ Submit online Home About Author Reviewer Reader Support Archives Search Home > Vol 70, No 1 (2014 > Urbaniak Original Research Bookmark and Share Freed by trust, to believe together: Pursuing global ecumenism with Küng and Tracy Jakub Urbaniak HTS Teologiese Studies / Theological Studies; Vol 70, No 1 (2014, 9 pages. doi: 10.4102/hts.v70i1.2047 Submitted: 16 August 2013 Published: 11 April 2014 Abstract In the past decades it has emerged more clearly than before that Christian religion, which has so often contributed to human oppression, has rich theological resources that can be used to restore and perfect human freedom. These resources have been reflected upon not only by liberation theologians, but also within the ecumenically oriented theology of religions which targets what Hans Küng calls global responsibility based on global ethics. World religions have an essential role to play in rendering that global humanity more humane and free. The only way to accomplish this task leads through ongoing dialogue, directed both ad intra and ad extra, in the pursuit of a ‘global ecumenism’ which the present suggests and the future demands. For those liberating and unitive resources inherent in religious theory and praxis to be activated, fundamental trust in the reality of the world and of one’s own self appears indispensable. By deepening the theological insights of Hans Küng and David Tracy, the article seeks to explore the mutual correlation between such fundamental trust in reality and religious faith in God, interpreted

  14. Defining the appropriate radiation dose for pretreatment PSA no. <=no. 10 ng/mL prostate cancer

    International Nuclear Information System (INIS)

    Purpose: To investigate whether a dose response exists for biochemical no evidence of disease (bNED) control in prostate cancer patients with pretreatment prostate-specific antigen (PSA) no. =no. 7600 cGy. Median dose in these three groups was 7067 cGy, 7278 cGy, and 7734 cGy, respectively. Univariate analysis was performed to determine differences in bNED control (American Society for Therapeutic Radiology and Oncology [ASTRO] Consensus Guidelines definition of failure) by dose group for the entire cohort, for 310 good prognosis patients (T1-T2A, Gleason score 2-6, absence of PNI), and for 178 poor prognosis patients (T2B-T3 or Gleason score 7-10 or presence of PNI) . Multivariate analysis (MVA) was performed to determine if dose was an independent predictor of bNED control. Median follow-up was 36 months. Results: A dose response was not demonstrated for the entire group of patients with pretreatment PSA no. =no. 7600 cGy were associated with 5-year bNED control rates of 73%, 86%, and 89%, respectively (p = 0.12). MVA demonstrated prognosis group (p = 0.038) to be the only independent predictor of bNED control. Good prognosis patients had a 5-year bNED of 85% and no dose response was seen. The subgroup of poor prognosis patients demonstrated a 5-year bNED control rate of 81% and a dose response was seen for those receiving no. >=no. 7600 cGy, compared to the two lower dose groups (94% vs. 75% vs. 70%; p = 0.0062). MVA for the poor prognosis subset demonstrated dose (p = 0.01) to be the only independent predictor for improved bNED control. Conclusions: The poor prognosis subset of PSA no. <=no. 10 ng/mL prostate cancer patients benefit from dose escalation. A dose response is not demonstrated for prostate cancer patients with pretreatment PSA no. <=no. 10 ng/mL and other favorable features

  15. Prediction of neutral noble gas insertion compounds with heavier pnictides: FNgY (Ng = Kr and Xe; Y = As, Sb and Bi).

    Science.gov (United States)

    Ghosh, Ayan; Manna, Debashree; Ghanty, Tapan K

    2016-04-28

    A novel class of interesting insertion compounds obtained through the insertion of a noble gas atom into the heavier pnictides have been explored by various ab initio quantum chemical techniques. Recently, the first neutral noble gas insertion compounds, FXeY (Y = P, N), were theoretically predicted to be stable; the triplet state was found to be the most stable state, with a high triplet-singlet energy gap, by our group. In this study, we investigated another noble gas inserted compound, FNgY (Ng = Kr and Xe; Y = As, Sb and Bi), with a triplet ground state. Density functional theory (DFT), second order Møller-Plesset perturbation theory (MP2), coupled-cluster theory (CCSD(T)) and multi-reference configuration interaction (MRCI) based techniques have been utilized to investigate the structures, stabilities, harmonic vibrational frequencies, charge distributions and topological properties of these compounds. These predicted species, FNgY (Ng = Kr and Xe; Y = As, Sb and Bi) are found to be energetically stable with respect to all the probable 2-body and 3-body dissociation pathways, except for the 2-body channel leading to the global minimum products (FY + Ng). Nevertheless, the finite barrier height corresponding to the saddle points of the compounds connected to their respective global minima products indicates that these compounds are kinetically stable. The structural parameters, energetics, and charge distribution results as well as atoms-in-molecules (AIM) analysis suggest that these predicted molecules can be best represented as F(-)[(3)NgY](+). Thus, all the aforementioned computed results clearly indicate that it may be possible to experimentally prepare the most stable triplet state of FNgY molecules under cryogenic conditions through a matrix isolation technique.

  16. Focused groundwater discharge of phosphorus to a eutrophic seepage lake (Lake Væng, Denmark)

    DEFF Research Database (Denmark)

    Kidmose, Jacob Baarstrøm; Nilsson, Bertel; Engesgaard, Peter Knudegaard;

    2013-01-01

    A study on Lake Væng in Denmark demonstrates a high potential for loading of phosphorous via groundwater to seepage lakes. Groundwater discharges are displayed as an important source of phosphorous to a lake due to: (1) high concentrations in the aquifer just below the lake, and (2) the main flow...... paths through the aquifer–lakebed interface either being overland flow through a seepage face, or focused in zones with very high discharge rates. In-lake springs have measured discharge of up to 7.45 m3 per m2 of lakebed per day. These findings were based on seepage meter measurements at 18 locations......, stable isotope (δ18O) analyses, temperature profiles and mapping of ice cover distribution. Groundwater–lake interaction was modelled with a 2D conceptual flow model (MODFLOW) with hydrogeology interpreted from catchment multi electrode profiling, on-lake ground-penetrating radar, well logging and...

  17. 40 Gb/s Lane Rate NG-PON using Electrical/Optical Duobinary, PAM-4 and Low Complex Equalizations

    DEFF Research Database (Denmark)

    Wei, J. L.; Grobe, Klaus; Wagner, Christoph;

    2016-01-01

    We present the first numerical investigation and comparison of 40-Gb/s lane rate electrical Duobinary, optical Duobinary and PAM-4 for NG-PONs incorporating low complex linear and nonlinear post-equalizations.......We present the first numerical investigation and comparison of 40-Gb/s lane rate electrical Duobinary, optical Duobinary and PAM-4 for NG-PONs incorporating low complex linear and nonlinear post-equalizations....

  18. Our Prostate Biopsy Results in The Patients with Prostate Specific Antigen Levels Below 4 ng/ml

    Directory of Open Access Journals (Sweden)

    Huseyin Aydemir

    2014-03-01

    Full Text Available Aim: The aim of this study is to evaluate the re¬sults of prostate biopsy of patients who had the prostate-specific antigen (PSA levels below 4 ng/ml. Material and Method: The medical records of 524 pa¬tients who underwent transrectal prostate biopsy be¬tween January 2010 and February 2013 in our clinic, due to suspi¬cion of prostate cancer were evaluated and histopathologic results of 43 patients whose PSA levels under 4 ng/ml were retrospectively revieved. Results: The mean age of patients was 64.63 ±7:42 and the mean level of PSA was 2.89 ±0.88ng /ml. A digital rectal examination (DRE had suspicious findings in 41(95.34% patients. Prostate adenocarcinoma was determined in 13 of (30.23% patients according to the biopsy result. The mean PSA value of these patients was 2.89 ±0.88 ng/ml and the mean gleason score of these patients was 6.41 ±0.87. The mean prostate volume of these patients was 41.46 ±11.95 cm3. Discussion: In our study, prostate cancer was identified in 30.3% of patients whose PSA levels were below 4 ng/ml. DRE, is still important for the evaluation of the prostate. According to our results, significant number of cancers can be detected in PSA below 4 ng/ml levels.

  19. Semiotics of Power and Dictatorship in Ngũgĩ wa Thiong’o’s Later Novels

    Directory of Open Access Journals (Sweden)

    Yémalo, C. AMOUSSOU

    2016-10-01

    Full Text Available This paper explores the different uses of symbols to express power and interpersonal relationship in Ngũgĩ’s bulkiest novel Wizard of the Crow (2006, with a few illustrations from Matigari (1987. It draws on the semiotic approach and identifies about a hundred discourse strings in which signs are used to express tenor between characters. In the main, supernatural, faunal and floral symbols are found to be main vehicles of power, though many other such avenues as ‘vocations’, ‘social and gender-related symbols’ and ‘biblical characterisation and numerology’, etc are unexplored here for space constraints. Before the analysis, it is deemed necessary to overview the theoretical background to the study in which relevant concepts have been clarified. It has been concluded that there is no other work in which power is so much expressed through the explored devices as in Wizard of the Crow.Keywords: tenor, symbol, index, proverb, modality metaphor, modaliser/ weakener, modulator/strengthener

  20. Snow avalanche history in Parâng Mountains (Southern Carpathians revealed by dendrogeomorphic analyses

    Directory of Open Access Journals (Sweden)

    Flaviu MESEȘAN

    2014-11-01

    Full Text Available Parâng Mountains belong to Southern Carpathians, having the maximum altitude in Parângul Mare Peak (2519 m a.s.l.. The geology is dominated by crystalline schists, due to whom the relief is characterized by steep slopes. Between 1400 – 1700 m the average annual temperature is around 2-3ᴼC and the total precipitation about 1000-1200 mm/year. Coniferous belt extends roughly at the mentioned altitudes, being dominated by Norway spruce (Picea abies forests. Above 1800 m the average annual temperature drops below 2ᴼC and the average rainfalls reach 1200 mm/year, allowing the development of alpine shrubs and alpine grassland (Oancea et al., 1987. Crests and steep slopes in these subalpine areas are prone to snow accumulation and avalanche release. Despite the favorable conditions for avalanche trigger in this area, only one avalanche event is historically recorded, with severe impact on forest areas, but no destructive impact on infrastructure or fatalities.

  1. Poly β-hydroxybutyrate production by Bacillus subtilis NG220 using sugar industry waste water.

    Science.gov (United States)

    Singh, Gulab; Kumari, Anish; Mittal, Arpana; Yadav, Anita; Aggarwal, Neeraj K

    2013-01-01

    The production of poly β-hydroxybutyrate (PHB) by Bacillus subtilis NG220 was observed utilizing the sugar industry waste water supplemented with various carbon and nitrogen sources. At a growth rate of 0.14 g h(-1) L(-1), using sugar industry waste water was supplemented with maltose (1% w/v) and ammonium sulphate (1% w/v); the isolate produced 5.297 g/L of poly β-hydroxybutyrate accumulating 51.8% (w/w) of biomass. The chemical nature of the polymer was confirmed with nuclear magnetic resonance, Fourier transform infrared, and GC-MS spectroscopy whereas thermal properties were monitored with differential scanning calorimetry. In biodegradability study, when PHB film of the polymer (made by traditional solvent casting technique) was subjected to degradation in various natural habitats like soil, compost, and industrial sludge, it was completely degraded after 30 days in the compost having 25% (w/w) moisture. So, the present study gives insight into dual benefits of conversion of a waste material into value added product, PHB, and waste management.

  2. Pagdalumat Sa Mga Sinaunang Di- Materyal Na Kultura Ng Mga B’laan Sa Brgy. Pisan, Kabacan, Cotabato Mula Sa Kanilang Kwentong Bayan

    Directory of Open Access Journals (Sweden)

    Dr. Nelia O. Du

    2014-12-01

    Full Text Available Nilayon ng pag-aaral na lumikom ng kwentong bayan ng mga B’laan sa Brgy. Pisan, Kabacan, North Cotabato at dalumatin ang kanilang di-materyal na kulturang masasalamin dito.Sa pagbibigay katuparan sa layunin, nasagot ang mga sumusunod na katanungan: (1 Ano ang mga kwentong bayan ng mga B’laan sa Pisan, Kabacan, Cotabato? (2 Anong mga di-materyal na kultura ang maaaring pagkakakilanlan ng mga B’laan ang masasalamin sa kanilang mga kwentong bayan? Paano ipinakita ang mga ito sa kanilang mga kwentong bayan? Saklaw ng pag-aaral na ito ang mga kwentong bayan na inuri ayon sa alamat, mito, salaysayin at pabula na kasasalaminan ng di-materyal na kultura ng mga B’laan na nakatira sa Brgy. Pisan, Kabacan, Cotabato. Kwantitatibo at Kwalitatibong disenyo ng pananaliksik ang ginamit sa pag-aaral at sinuri ang mga datos sa pamamagitan ng deskriptibong pamaraan partikular ang kontent analisis. Pamaraang indihenus o pangkatutubo ang pamaraang ginamit. Natuklasan na (1 May iba’t ibang kwentong bayan sa genre na alamat na etiolohikal, mito, salaysayin, at pabula ang mga Blaan; (2 Iba’t ibang di-materyal na kultura ang masasalamin sa mga kwentong bayan ng B’laan. Ito ang nabuong konklusyon: (1 Ang mga kwentong bayan ng B’laan ay kasasalaminan ng kanilang di-materyal na kultura.

  3. Nitrofurazone quantification in milk at the European Union minimum required performance limit of 1 ng g(-1): circumventing the semicarbazide problem.

    Science.gov (United States)

    Pearson, Ross A; Evans, Cameron; Bendall, Justin G

    2016-08-01

    Nitrofurazone is an antibiotic with carcinogenic properties. Efforts by regulatory authorities to control nitrofurazone from agricultural foods are an important public health measure that have, to some extent, been undermined by widespread use amongst laboratories of the unreliable marker metabolite semicarbazide. This work confirms what has long been suspected, namely that powdered dairy products that are initially free of semicarbazide develop semicarbazide under storage conditions such as occur normally across commercial supply chains. The low ng g(-)(1) levels of semicarbazide formed in this way are insufficient to present any food safety hazard. That such development of a marker metabolite is demonstrated to occur by innocent means effectively invalidates the use of semicarbazide as a marker metabolite for powdered dairy products, and exacerbates the regulatory need for a more suitable analytical methodology. In milk, unlike meat, nitrofurazone is known to remain stable and thus available for analysis in the intact form, rather than necessitating any use of a metabolite or fragment. However, no previous methodology that was capable of achieving the stringent European minimum required performance limit of 1 ng g(-)(1) when using intact nitrofurazone had been described for milk. This work describes a specific methodology using LC-MS/MS for milk and milk powder; it achieves detection of intact nitrofurazone (as well as furazolidone, furaltadone and nitrofurantoin) to levels well below 1 ng g(-)(1). Laboratories will no longer need to use semicarbazide as an unreliable marker metabolite for the analysis of nitrofurazone in dairy products, paving the way for regulatory authorities to better control nitrofurazone abuse with greater confidence. PMID:27455411

  4. Mutation of NgBR, a subunit of cis-prenyltransferase, causes a congenial disorder of glycosylation

    Science.gov (United States)

    Park, Eon Joo; Grabińska, Kariona A.; Guan, Ziqiang; Stránecký, Viktor; Hartmannová, Hana; Hodaňová, Kateřina; Barešová, Veronika; Sovová, Jana; Jozsef, Levente; Ondrušková, Nina; Hansíková, Hana; Honzík, Tomáš; Zeman, Jiří; Hůlková, Helena; Wen, Rong; Kmoch, Stanislav; Sessa, William C.

    2014-01-01

    Summary Dolichol is an obligate carrier of glycans for N-linked protein glycosylation, O-mannosylation, and GPI anchor biosynthesis. Cis-prenyltransferase (cis-PTase) is the first enzyme committed to the synthesis of dolichol. However, the proteins responsible for mammalian cis-PTase activity have not been delineated. Here we show that Nogo-B receptor (NgBR) is a subunit required for dolichol synthesis in yeast, mice and man. Moreover, we describe a family with a congenital disorder of glycosylation caused by a loss of function mutation in the conserved C terminus of NgBR-R290H and show that fibroblasts isolated from patients exhibit reduced dolichol profiles and enhanced accumulation of free cholesterol identically to fibroblasts from mice lacking NgBR. Mutation of NgBR-R290H in man and orthologs in yeast proves the importance of this evolutionarily conserved residue for mammalian cis-PTase activity and function. Thus, these data provides a genetic basis for the essential role of NgBR in dolichol synthesis and protein glycosylation. PMID:25066056

  5. Matrix effect on vibrational frequencies: experiments and simulations for HCl and HNgCl (Ng = Kr and Xe).

    Science.gov (United States)

    Kalinowski, Jaroslaw; Gerber, R Benny; Räsänen, Markku; Lignell, Antti; Khriachtchev, Leonid

    2014-03-01

    We study the environmental effect on molecules embedded in noble-gas (Ng) matrices. The experimental data on HXeCl and HKrCl in Ng matrices is enriched. As a result, the H-Xe stretching bands of HXeCl are now known in four Ng matrices (Ne, Ar, Kr, and Xe), and HKrCl is now known in Ar and Kr matrices. The order of the H-Xe stretching frequencies of HXeCl in different matrices is ν(Ne) Kr) Kr) Kr stretching frequencies of HKrCl is consistently ν(Kr) < ν(Ar). These matrix effects are analyzed theoretically by using a number of quantum chemical methods. The calculations on these molecules (HCl, HXeCl, and HKrCl) embedded in single Ng(') layer cages lead to very satisfactory results with respect to the relative matrix shifts in the case of the MP4(SDQ) method whereas the B3LYP-D and MP2 methods fail to fully reproduce these experimental results. The obtained order of frequencies is discussed in terms of the size available for the Ng hydrides in the cages, probably leading to different stresses on the embedded molecule. Taking into account vibrational anharmonicity produces a good agreement of the MP4(SDQ) frequencies of HCl and HXeCl with the experimental values in different matrices. This work also highlights a number of open questions in the field.

  6. The crossover from classical to quantum behavior in Duffing oscillator based on quantum state diffusion

    Indian Academy of Sciences (India)

    Y Ota; I Ohba

    2002-08-01

    The classical Duffing oscillator is a dissipative chaotic system, and so there is not a definite Hamiltonian. We quantize the Duffing oscillator on the basis of quantum state diffusion (QSD) which is a formulation for open quantum systems and a useful tool for analyzing nonlinear problems and classical limits. We can define a ‘Lyapunov exponent’, which corresponds to the classical one in the proper limit, and investigate the behavior of the system by varying the Planck constant effectively. We show that there exists a critical stage, where the behavior of the system crosses over from classical to quantum one.

  7. 游离NgR促进新生大鼠背根神经节突起体外生长%Role of soluble NgR in promotion of DRG neurite sprouting in vitro in rats

    Institute of Scientific and Technical Information of China (English)

    马永刚; 刘世清; 范里; 陶海鹰; 卫爱林

    2009-01-01

    目的 观察生物合成的游离NgR对新生大鼠原代背根神经节(DRG)神经元突起生长的影响.方法 以ViraPowerTM慢病毒将NgR(310)ecto基因转染人培养的骨髓基质细胞,收集培养上清获得游离NgR.分离新生大鼠DRG神经元并分为两组,对照组直接将神经元种植到经含中枢神经系统(CNS)髓鞘蛋白包被的培养皿中培养;实验组先将骨髓基质细胞培养上清加入含CNS髓鞘蛋白的培养皿中,孵育4 h后再植入DRG神经元.两组细胞均在培养24 h后固定,进行βⅢ-tubulin免疫组织化学染色,图像观察神经元及其突起生长情况.结果 基因修饰的骨髓基质细胞,转染后48 h间接免疫荧光的方法检测可见胞浆内荧光表达.种植24 h后对照组DRG细胞很少有突起长出;而实验组DRG细胞中60%的神经元长出突起,且突起较长.结论 游离NgR能够竞争性结合脊髓损伤后局部分泌的轴突生长抑制因子,从而保护生理性NgR,可能是促轴突再生和脊髓功能恢复的一种新策略.%Objective To observe the effect of soluble NgR on rat DRG neurite sprouting in vitro. Methods Gene encoding NgR(310)ecto was transduced into bone marrow stromal cells (BM-SCs). Soluble NgR was obtained from the supernatant of BMSCs culture. DRG neurons of neonate rat were isolated and allocated into two groups, ie, control group (DRG neurons were cultured in slides coa-ted with myelin protein of spinal cord) and experiment group (the slides were pre-incubated with the su-pernatant of BMSCs for four hours). BMSCs were immobilized after 24 hours of culture. Immunohis-tostaining with βⅢ-tubulin and fluorescence microscope were employed to analyze the neurite growth.Results Positive staining of intracytoplasm was found with indirect fluorescence microscope in the BM-SCs 48 hours following transduction. Little neurite was found sprout to DRG neurons in the control group 24 hours after transduction. In contrast, longer neurite was observed

  8. Pagdalumat Sa Mga Sinaunang Di- Materyal Na Kultura Ng Mga B’laan Sa Brgy. Pisan, Kabacan, Cotabato Mula Sa Kanilang Kwentong Bayan

    OpenAIRE

    Dr. Nelia O. Du

    2014-01-01

    Nilayon ng pag-aaral na lumikom ng kwentong bayan ng mga B’laan sa Brgy. Pisan, Kabacan, North Cotabato at dalumatin ang kanilang di-materyal na kulturang masasalamin dito.Sa pagbibigay katuparan sa layunin, nasagot ang mga sumusunod na katanungan: (1) Ano ang mga kwentong bayan ng mga B’laan sa Pisan, Kabacan, Cotabato? (2) Anong mga di-materyal na kultura ang maaaring pagkakakilanlan ng mga B’laan ang masasalamin sa kanilang mga kwentong bayan? Paano ipinakita ang mga ito sa ...

  9. Potential of the multispectral synergism for observing ozone pollution combining measurements of IASI-NG and UVNS onboard EPS-SG

    Science.gov (United States)

    Costantino, Lorenzo; Cuesta, Juan; Emili, Emanuele; Foret, Gilles; Dufour, Gaëlle; Eremenko, Maxim; Chailleux, Yohann; Beekmann, Matthias; Flaud, Jean-Marie

    2016-04-01

    Current and future satellite observations offer a great potential for monitoring air quality on daily and global basis. However, measurements from currently in orbit sensors offer a limited capacity to probe surface concentrations of gaseous pollutants such as tropospheric ozone. Using single-band approaches based on IASI spaceborne thermal infrared measurements, only ozone down to the lower troposphere (3-4 km of altitude at lowest) may be observed (Eremenko et al., 2008). A recent multispectral method combining IASI and GOME-2 (both onboard MetOp satellites) spectra, respectively from the IR and UV, has shown enhanced sensitivity for probing ozone at the lowermost troposphere, but with maximum sensitivity around 2 km at lowest (Cuesta et al., 2013). Future spatial missions will be launched in the upcoming years, such as EPS-SG, carrying new generation sensors like IASI-NG and UVNS that will enhance the capacity to observe ozone pollution, and particularly when combining them through a multispectral synergism. This work presents an analysis of the potential of the multispectral synergism of IASI-NG and UVNS future spaceborne measurements for observing ozone pollution, performed in the framework of SURVEYOZON project (funded by the French Space Agency, CNES). For this, we develop a simulator of synthetic multispectral retrievals or pseudo-observations (referred as OSSE, Observing System Simulation Experiment) derived from IASI-NG+UVNS that will be compared to those from IASI+GOME2. In the first step of the OSSE, we create a pseudo-reality with simulations from the chemical-transport model MOCAGE (provided by CERFACS laboratory), where real O3 data from IASI and surface network stations have been assimilated for a realistic representation of ozone variability at the surface and the free troposphere. We focus on the high pollution event occurred in Europe on 10 July 2010. We use the coupled algorithms KOPRA+VLIDORT to simulate the spectra emitted, scattered and

  10. Kui naine tahab olla kangelane : kestvus-perfomance "Äng - 161 km palja rindkerega" / Fideelia-Signe Roots

    Index Scriptorium Estoniae

    Roots, Fideelia-Signe, 1976-

    2014-01-01

    Fideelia-Signe Roots oma aktsioonist tegevuskunstiürituse "Äng" raames, mille käigus kunstnik kõndis jalgsi alasti ülakehaga 161 kilomeetrit Tartust Karepale eesmärgiga testida naise kehakuvandi piire. Kangelase ja superkangelase mõistest

  11. The Chances of Subsequent Cancer Detection in Patients with a PSA > 20 ng/ml and an Initial Negative Biopsy

    Directory of Open Access Journals (Sweden)

    Nadeem Shaida

    2009-01-01

    Full Text Available Transrectal ultrasound (TRUS–guided prostate biopsy is known to carry a significant false-negative rate, leading some patients to have multiple biopsies. We investigated cancer detection rates in patients with a PSA >20 ng/ml and a negative initial biopsy. We reviewed our database of 2396 TRUS-guided biopsies done between 1997 and 2002 in order to give a follow-up of at least 6 years. PSA, PSA density (PSAD, PSA velocity (PSAV, prostate volume, and DRE findings were analysed in relation to cancer status. Of the patients, 388 (16% had a PSA >20 ng/ml, including 99 (26% with benign biopsies. Of those, 67 were rebiopsied, including 19 (28% with cancer on the first rebiopsy and four (6% on further biopsies. PSAD, DRE, and volume significantly differed between rebiopsied patients with and without cancer (p 20 ng/ml and have an initial negative biopsy have a high chance of malignancy being detected on a second biopsy. However, if a second biopsy is also negative, then the chances of subsequent biopsies showing signs of cancer are very low if the DRE is normal and particularly if the PSAD is >0.35 ng/ml/cm3.

  12. Research on General Corrosion Property of 304L and 304NG Stainless Steels in Simulated PWR Primary Water

    Institute of Scientific and Technical Information of China (English)

    PENG; De-quan; HU; Shi-lin; ZHANG; Ping-zhu; WANG; Hui

    2012-01-01

    <正>The general corrosion behaviors of 304L and 304NG grade stainless steels in simulated pressurized water reactor (PWR) primary loop were studied using still autoclave, respectively, the corrosion test lasted for 1 680 hours. The corrosion oxide films were analyzed macroscopically and microscopically. The results are shown in Figs. 1, 2.

  13. Pentacyclic ingamine-type alkaloids, a new antiplasmodial pharmacophore from the marine sponge petrosid Ng5 Sp5

    Science.gov (United States)

    Two new pentacyclic ingamine- type alkaloids, namely 22(S)-hydroxyingamine A (2) and dihydroingenamine D (3), together with the known ingamine A (1) have been isolated from marine sponge Petrosid Ng5 Sp5 (Family: Petrosiidae) obtained from the open repository of National Cancer Institute, USA. The s...

  14. Draft Genome Sequence of Neisseria gonorrhoeae Strain NG_869 with Penicillin, Tetracycline and Ciprofloxacin Resistance Determinants Isolated from Malaysia.

    Science.gov (United States)

    Ang, Geik Yong; Yu, Choo Yee; Yong, Delicia Ann; Cheong, Yuet Meng; Yin, Wai-Fong; Chan, Kok-Gan

    2016-06-01

    Gonorrhea is a sexually transmitted infection caused by Neisseria gonorrhoeae and the increasing reports of multidrug-resistant gonococcal isolates are a global public health care concern. Herein, we report the genome sequence of N. gonorrhoeae strain NG_869 isolated from Malaysia which may provide insights into the drug resistance determinants in gonococcal bacteria. PMID:27570316

  15. Health Information in Vietnamese (Tiếng Việt): MedlinePlus

    Science.gov (United States)

    ... Tiếng Việt (Vietnamese) Bilingual PDF Health Information Translations Circumcision Male Circumcision Cắt Bao Quy Đầu cho Em Bé Trai - ... Control and Prevention Drug Abuse Drugs, Alcohol and HIV/AIDS: A Consumer Guide - English Rượu & Ma Túy ...

  16. Multi-analyte approach for the determination of ng L{sup -1} levels of steroid hormones in unidentified aqueous samples

    Energy Technology Data Exchange (ETDEWEB)

    Noppe, H. [Ghent University, Faculty of Veterinary Medicine, Research group of Veterinary Public Health and Zoonoses, Laboratory of Chemical Analysis, Salisburylaan 133, B-9820 Merelbeke (Belgium)]. E-mail: Herlinde.Noppe@UGent.be; Verheyden, K. [Ghent University, Faculty of Veterinary Medicine, Research group of Veterinary Public Health and Zoonoses, Laboratory of Chemical Analysis, Salisburylaan 133, B-9820 Merelbeke (Belgium); Gillis, W. [Federal Agency for the Safety of the Food Chain, WTC III, Simon Bolivarlaan 30, B-1000 Brussels (Belgium); Courtheyn, D. [Federal Feed and Food Laboratory, Braemkasteelstraat 59, B-9050 Ghent (Belgium); Vanthemsche, P. [Federal Agency for the Safety of the Food Chain, WTC III, Simon Bolivarlaan 30, B-1000 Brussels (Belgium); De Brabander, H.F. [Ghent University, Faculty of Veterinary Medicine, Research group of Veterinary Public Health and Zoonoses, Laboratory of Chemical Analysis, Salisburylaan 133, B-9820 Merelbeke (Belgium)

    2007-03-14

    Since the 1970s, many analytical methods for the detection of illegal growth promoters, such as thyreostats, anabolics, {beta}-agonists and corticosteroids have been developed for a wide range of matrices of animal origin, including meat, fat, organ tissue, urine and faeces. The aim of this study was to develop an analytical method for the determination of ng L{sup -1} levels of estrogens, gestagens, androgens (EGAs) and corticosteroids in aqueous preparations (i.e. drinking water, drinking water supplements), commercially available on the 'black' market. For this, extraction was performed with Bakerbond C{sub 18} speedisk, a technique commonly used in environmental analysis. After fractionation, four fractions were collected using a methanol:water gradient program. Gas chromatography coupled to electron impact multiple mass spectrometry (GC-EI-MS{sup 2}) screening for the EGAs was carried out on the derivatized extracts. For the detection of corticosteroids, gas chromatography coupled to negative chemical ionization mass spectrometry (GC-NCI-MS) was used after oxidation of the extracts. Confirmation was done by liquid chromatography coupled to electrospray ionization multiple mass spectrometry (LC-ESI-MS{sup 2}). The combined use of GC and LC coupled to MS enabled the identification and quantification of anabolics and corticosteroids at the low ng L{sup -1} level. This study demonstrated the occurrence of both androgens and corticosteroids in different commercial aqueous samples.

  17. Nogo-A及NgR在墨西哥钝口螈脑组织中的表达及意义%Expression of Nogo-A and NgR and its significance in the Ambystoma mexicanum brain tissue

    Institute of Scientific and Technical Information of China (English)

    钟玉华; 李敏; 刘佳; 彭福华

    2014-01-01

    目的 探讨勿动蛋白A(Nogo-A)及勿动蛋白受体(NgR)在正常墨西哥钝口螈及其中脑胆碱能神经元损伤后脑组织中的表达.方法 以正常墨西哥钝口螈脑组织和中脑胆碱能神经元损伤后第3、7、14、21天脑组织作为研究对象,采用免疫荧光技术观察正常墨西哥钝口螈脑组织中Nogo-A及NgR的表达;分别用荧光定量PCR和蛋白质印迹法(WB法)检测正常墨西哥钝口螈和损伤组脑组织中Nogo-A及NgR的mRNA含量和蛋白含量的变化.结果 正常墨西哥钝口螈脑组织中存在Nogo-A及NgR的表达,在墨西哥钝口螈中脑胆碱能神经元损伤后(注入AF64A)第3天,中脑乙酰胆碱转移酶(ChAT)阳性细胞数明显减少,与正常组比较差异有统计学意义(P<0.01),损伤后第7、14、21天均有ChAT、5-溴脱氧尿嘧啶核苷(BrdU)双阳性细胞,且随时间的延长而增加.中脑胆碱能神经元损伤后第3、7、14、21天脑组织中,Nogo-A的mRNA和蛋白水平的表达量与正常组比较差异均无统计学意义;而NgR的mRNA和蛋白水平的表达量均下降,与正常组比较差异有统计学意义(P<0.05).结论 中枢神经系统损伤后的再生可能与NgR表达量的下降有关.

  18. Charonosaurus jiayinensis n.g., n.sp.,a lambeosaurine dinosaur from the Late Maastrichtian of northeastern ChinaCharonosaurus jiayinensis n.g., n.sp., un dinosaure lambéosauriné du Maastrichtien supérieur du Nord-Est de la Chine.

    Science.gov (United States)

    Godefroit, Pascal; Zan, Shuqin; Jin, Liyong

    2000-06-01

    Large bonebeds have been excavated in the Late Maastrichian Yuliangze Formation near Jiayin (Heilongjang Province, northeastern China). The greatest part of the discovered fossils belong to a new lambeosaurine dinosaur, Charonosaurus jiayinensis n.g., n.sp. Phylogenetic analysis, based on 33 cranial, dental and postcranial characters, indicates that this new taxon may be closer to Parasaurolophus than any other known Lambeosaurinae. The Late Maastrichtian dinosaur faunas currently known in northeastern Asia are dominated by Lambeosaurinae, although these dinosaurs are not represented any more in synchronous North American and European localities.

  19. Potential of the future thermal infrared space-borne sensor IASI-NG to monitor lower tropospheric ozone

    Directory of Open Access Journals (Sweden)

    P. Sellitto

    2012-09-01

    Full Text Available The lower tropospheric (LT ozone concentration is a key factor for air quality (AQ. Observing efficiently LT ozone from space is crucial to monitor and better understand pollution phenomena occurring from inter-continental to local scales, and that have a proven noxious effect on the human health and the biosphere. The Infrared Atmospheric Sounder Interferometer (IASI flies on MetOp-A spacecraft and is planned to be launched in the next future as part of the other MetOp modules, i.e. MetOp-B and C. IASI has demonstrated to have the capability to single out the LT ozone signal only at favourable conditions, i.e. in presence of high thermal contrast scenarios. New generation satellite instruments are being designed to address several pressing geophysical issues, including a better observation capability of LT ozone. IASI-NG (New Generation, now having reached the accomplishment of design phase-A for launch in the 2020 timeframe as part of the EPS-SG (EUMETSAT Polar System-Second Generation, formerly post-EPS mission, may render feasible a better observation of AQ in terms of LT ozone. To evaluate the added-value brought by IASI-NG in this context, we developed a pseudo-observation simulator, including a direct simulator of thermal infrared spectra and a full inversion scheme to retrieve ozone concentration profiles. We produced one month (August 2009 of tropospheric ozone pseudo-observations based on both IASI and IASI-NG instrumental configurations. We compared the pseudo-observations and we found a clear improvement of LT ozone (up to 6 km altitude pseudo-observations quality for IASI-NG. The estimated total error is expected to be more than 35% smaller at 5 km, and 20% smaller for the LT ozone column. The total error on the LT ozone column is, on average, lower than 10% for IASI-NG. IASI-NG is expected to have a significantly better vertical sensitivity (monthly average degrees of freedom surface-6 km of 0.70 and to be sensitive at lower

  20. Potential of the future thermal infrared space-borne sensor IASI-NG to monitor lower tropospheric ozone

    Science.gov (United States)

    Sellitto, P.; Dufour, G.; Eremenko, M.; Cuesta, J.; Dauphin, P.; Forêt, G.; Gaubert, B.; Beekmann, M.; Peuch, V.-H.; Flaud, J.-M.

    2012-09-01

    The lower tropospheric (LT) ozone concentration is a key factor for air quality (AQ). Observing efficiently LT ozone from space is crucial to monitor and better understand pollution phenomena occurring from inter-continental to local scales, and that have a proven noxious effect on the human health and the biosphere. The Infrared Atmospheric Sounder Interferometer (IASI) flies on MetOp-A spacecraft and is planned to be launched in the next future as part of the other MetOp modules, i.e. MetOp-B and C. IASI has demonstrated to have the capability to single out the LT ozone signal only at favourable conditions, i.e. in presence of high thermal contrast scenarios. New generation satellite instruments are being designed to address several pressing geophysical issues, including a better observation capability of LT ozone. IASI-NG (New Generation), now having reached the accomplishment of design phase-A for launch in the 2020 timeframe as part of the EPS-SG (EUMETSAT Polar System-Second Generation, formerly post-EPS) mission, may render feasible a better observation of AQ in terms of LT ozone. To evaluate the added-value brought by IASI-NG in this context, we developed a pseudo-observation simulator, including a direct simulator of thermal infrared spectra and a full inversion scheme to retrieve ozone concentration profiles. We produced one month (August 2009) of tropospheric ozone pseudo-observations based on both IASI and IASI-NG instrumental configurations. We compared the pseudo-observations and we found a clear improvement of LT ozone (up to 6 km altitude) pseudo-observations quality for IASI-NG. The estimated total error is expected to be more than 35% smaller at 5 km, and 20% smaller for the LT ozone column. The total error on the LT ozone column is, on average, lower than 10% for IASI-NG. IASI-NG is expected to have a significantly better vertical sensitivity (monthly average degrees of freedom surface-6 km of 0.70) and to be sensitive at lower altitudes (more

  1. Løssluppen kohærens i Fredmans säng nr. 41 "Joachim uti Babylon"

    DEFF Research Database (Denmark)

    Heltoft, Lars

    2015-01-01

    Denne artikel er ment som et bud på at løse visse problemer med forståelsen af FS (Fredmans Sång) 41 som en del af de bibelske travestier i værket. Den er samtidig et bidrag til udviklingen af tekstanalytisk praksis på grundlag af tæt sproglig analyse. Den er et eksempel på hvorledes tekstforståe......Denne artikel er ment som et bud på at løse visse problemer med forståelsen af FS (Fredmans Sång) 41 som en del af de bibelske travestier i værket. Den er samtidig et bidrag til udviklingen af tekstanalytisk praksis på grundlag af tæt sproglig analyse. Den er et eksempel på hvorledes...

  2. Along the Road: The Ngäbe-Buglé Struggle to Protect Environmental Resources in Panama

    Directory of Open Access Journals (Sweden)

    Rogelio Cansari

    2013-09-01

    Full Text Available Indigenous people in Panama do not enjoy full autonomy within their comarcas (traditional land reserves: they only control surface resources, while the state retains control of underground resources. This article analyses direct action by the Ngäbe-Buglé, who successfully defeated the latest attempt by the government to exploit underground resources within their comarcas. It describes government strategies for retaining control over Indigenous people’s land and analyses how the Ngäbe-Buglé counteracted these strategies with support from burgeoning civil society movements. We argue that this is due to an unprecedented alliance between Indigenous people and other social movements in Panama, as well as to the fact that Indigenous people have succeeded in federating all major civil society organizations around their discourses and actions.

  3. Microglia/monocytes with NG2 expression have no phagocytic function in the cortex after LPS focal injection into the rat brain.

    Science.gov (United States)

    Zhu, Lie; Xiang, Ping; Guo, Kun; Wang, Anni; Lu, Jia; Tay, Samuel Sam Wah; Jiang, Hua; He, Bei Ping

    2012-09-01

    While OX42(+) microglia/macrophages have been considered as a scavenger in the brain, NG2(+) cells are generally considered as oligodendrocyte progenitor cells or function-unknown glial cells. Recent evidence showed that under some pathological conditions, certain cells have become positive for both anti-NG2 and anti-OX42 antibodies. Our results suggested that some OX42(+) microglia or macrophages were induced to express NG2 proteins 3 and 5 days later after focal injection of lipopolysaccharide into the brain cortex of Sprague-Dawley rats. In consideration of the induction of NG2 expression may associate with gaining or losing functions of microglia/macrophages, we further showed that, while OX42(+) or ED1(+) microglia/macrophages presented active phagocytic function, NG2(+) /OX42(+) cells failed to engulf latex beads. The induced expression of NG2 protein may possibly indicate the functional diversity of activated microglia/macrophages in the brain.

  4. Voltage-gated sodium channel expression and action potential generation in differentiated NG108-15 cells

    OpenAIRE

    Liu Jinxu; Tu Huiyin; Zhang Dongze; Zheng Hong; Li Yu-Long

    2012-01-01

    Abstract Background The generation of action potential is required for stimulus-evoked neurotransmitter release in most neurons. Although various voltage-gated ion channels are involved in action potential production, the initiation of the action potential is mainly mediated by voltage-gated Na+ channels. In the present study, differentiation-induced changes of mRNA and protein expression of Na+ channels, Na+ currents, and cell membrane excitability were investigated in NG108-15 cells. Result...

  5. Performance of the Abbott RealTime CT/NG for Detection of Chlamydia trachomatis and Neisseria gonorrhoeae▿

    OpenAIRE

    Gaydos, C A; Cartwright, C P; Colaninno, P.; Welsch, J.; Holden, J; Ho, S. Y.; Webb, E. M.; Anderson, C.; Bertuzis, R.; Zhang, L.; Miller, T; Leckie, G.; Abravaya, K.; Robinson, J.

    2010-01-01

    A multicenter clinical study was conducted to evaluate the performance characteristics of the Abbott RealTime CT/NG assay, a multiplex real-time PCR assay, for simultaneous detection of Chlamydia trachomatis and Neisseria gonorrhoeae. The specimens were collected from a total of 3,832 male and female subjects at 16 geographically diverse sites. Specimens included male and female urine samples, male urethral swabs, female endocervical swabs, and self-collected and clinician-collected vaginal s...

  6. Functional expression of human D3 dopamine receptors in differentiated neuroblastoma x glioma NG108-15 cells.

    OpenAIRE

    Seabrook, G. R.; Kemp, J.A.; Freedman, S B; Patel, S.; Sinclair, H. A.; McAllister, G.

    1994-01-01

    This study describes the depression of calcium currents caused by activation of human D3 dopamine receptors which have been stably expressed in the neuroblastoma x glioma NG108-15 cell line. Transfected cells, which had been differentiated with prostaglandin E1 and isobutylmethylxanthine, exclusively expressed D3 receptor mRNA, which was demonstrated by reverse transcription polymerase chain reaction techniques. Transfected cells had high affinity binding sites for iodosulpiride, with a Kd of...

  7. Mesenchymal Tumors Can Derive from Ng2/Cspg4-Expressing Pericytes with β-Catenin Modulating the Neoplastic Phenotype

    Directory of Open Access Journals (Sweden)

    Shingo Sato

    2016-07-01

    Full Text Available The cell of origin for most mesenchymal tumors is unclear. One cell type that contributes to this lineages is the pericyte, a cell expressing Ng2/Cspg4. Using lineage tracing, we demonstrated that bone and soft tissue sarcomas driven by the deletion of the Trp53 tumor suppressor, or desmoid tumors driven by a mutation in Apc, can derive from cells expressing Ng2/Cspg4. Deletion of the Trp53 tumor suppressor gene in these cells resulted in the bone and soft tissue sarcomas that closely resemble human sarcomas, while stabilizing β-catenin in this same cell type caused desmoid tumors. Comparing expression between Ng2/Cspg4-expressing pericytes lacking Trp53 and sarcomas that arose from deletion of Trp53 showed inhibition of β-catenin signaling in the sarcomas. Activation of β-catenin inhibited the formation and growth of sarcomas. Thus, pericytes can be a cell of origin for mesenchymal tumors, and β-catenin dysregulation plays an important role in the neoplastic phenotype.

  8. Typing of Neisseria gonorrhoeae Opa and NG-MAST Gene of 12 Pairs of Sexual Contact Gonorrhea Patients in China

    Institute of Scientific and Technical Information of China (English)

    Hongxiang CHEN; Jiawen LI; Zhihong WU; Rongyi CHEN; Li XU; Ying YU; Jun SHUAI; Juan LI; Ming TAN; Yating TU

    2008-01-01

    To identify the genomic species of Neisseria gonorrhoeae, evaluate the difference between two molecular epidemiological methods and examine the relationship between sex partners and genotypes of bacteria, 24 strains of Neisseria gonorrhoeae isolated from the outpatients with gonorrhea were identified by using the Opa genotyping and NG-MAST genotyping and the relationship between genotypes and phenotypes was studied. Twenty-four strains of Neisseria gonorrhoeae fell into 10 ST genotypes by NG-MAST genotyping, whereas these strains were classified into 12 OT Opa genotypes by Opa genotyping. A new epidemic strain of ST genotype (217-86% homologisation 178) in China was identified. It is concluded that genotypes of each pair of strains from a pair of patient/sex partner besides 45/46 are the same, indicating that contagious infection take place between patient and the sex partner. Opa genotyping was more effective than NG-MAST genotyping in identifying the genomic species of Neisseria gonorrhoeae. ST genotype could be further classified into different Opa-types.

  9. Developing a follow-up strategy for patients with PSA ranging from 4 to 10 ng/ml via a new model to reduce unnecessary prostate biopsies.

    Directory of Open Access Journals (Sweden)

    Ruizhe Zhao

    Full Text Available OBJECTIVE: The aim of this study was to develop a follow-up strategy based on the new model to reduce unnecessary prostate biopsies in patients with prostate specific antigen (PSA ranging from 4 to 10 ng/ml. METHODS: A total of 436 patients with PSA ranging from 4 to 10 ng/ml who had undergone transrectal ultrasound (TRUS-guided prostate biopsy were evaluated during the first stage. Age, PSA, free PSA (fPSA, digital rectal examination (DRE findings, ultrasonic hypoechoic mass, ultrasonic microcalcifications, prostate volume (PV and PSA density (PSAD were considered as predictive factors. A multiple logistic regression analysis involving a backward elimination selection procedure was applied to select independent predictors. After a comprehensive analysis of all results, we developed a new model to assess the risk of prostate cancer and an effective follow-up strategy. RESULTS: Age, PSA, PV, fPSA, rate of abnormal DRE findings and rate of hypoechoic masses detected by TRUS were included in our model. A significantly greater area under the receiver-operating characteristic curve was obtained in our model when compared with using PSA alone (0.782 vs. 0.566. Patients were grouped according to the value of prostate cancer risk (PCaR. In the second stage of our study, patients with PCaR>0.52 were recommended to undergo biopsies immediately while the rest of the patients continued close follow-up observation. Compared with the first stage, the detection rate of PCa in the second stage was significantly increased (33.0% vs 21.1%, p = 0.012. There was no significant difference between the two stages in distribution of the Gleason score (p = 0.808. CONCLUSIONS: We developed a follow-up strategy based on the new model, which reduced unnecessary prostate biopsies without delaying patients' diagnoses and treatments.

  10. Contato, epidemias e corpo como agentes de transformação: um estudo sobre a AIDS entre os Índios Xokléng de Santa Catarina, Brasil Contact, epidemics, and the body as agents of change: a study of AIDS among the Xokléng indians in the State of Santa Catarina, Brazil

    Directory of Open Access Journals (Sweden)

    Flávio Braune Wiik

    2001-03-01

    Full Text Available A partir da análise dos episódios de AIDS que acometeram os Índios Xokléng em 1988, o presente artigo visa referir fenômenos de doença a rupturas e transformações socioculturais ocorridas nesse universo com base na história de contato com a sociedade abrangente. A interpretação e a análise da relação estabelecida são feitas à luz de teorias antropológicas acerca da centralidade que corpo, corporalidade e processos corporais degenerativos ocupam nas sociedades indígenas brasileiras. Propõe-se que corpo, sociedade e agentes macroconjunturais são articulados pela práxis; por isso, devem ser relacionados nos estudos socio-antropológicos concernentes aos fenômenos de doença. O artigo traz a descrição sucinta das epidemias advindas com o contato, tentando vinculá-las aos contextos históricos específicos que o marcaram. Categorias da etnomedicina, cosmologia e corporalidade Xokléng são associadas à organização social e aos casos de AIDS. Estes são apresentados tendo, como enfoque principal, a ligação estabelecida entre seu advento e as transformações no universo Xokléng em função da construção da Barragem Norte junto a sua Terra.Based on an analysis of AIDS cases among the Xokléng Indians in 1988, this article relates the illness phenomenon to socio-cultural disruptions and transformations in this indigenous group's universe, focusing on the history of their contact with Brazilian national society. The analysis and interpretation of this relationship are based on anthropological theories about the centrality of the body, corporeality, and degenerative bodily processes in Brazilian indigenous societies, according to which the body, society, and macro-situational elements are articulated by social praxis, and should thus be related in socio-anthropological studies of health-illness phenomena. The article briefly describes the history of epidemics emerging from contact and attempts to relate them to specific

  11. Meditation In The Emotional Intelligence Improvement Among Russian-Speakıng Migrants In Germany

    OpenAIRE

    Sergey Afanasyev

    2014-01-01

    The analysis of meditation as a factor of an individual’s psychological and emotional well-being as well as the comparison of main emotional intelligence quantitative measurement methods and the justification of the method used in the study were performed. Main features of the migrants’ social-psychological adaptation were considered. The key role of interpersonal emotional intelligence competency in migrants’ social-psychological adaptation process was identified. The interrelation between m...

  12. Pagsusuri sa mga Balyung Nakapaloob sa mga Salawikain ng mga Tiruray Sa South Upi, Maguindanao, Philippines

    Directory of Open Access Journals (Sweden)

    Maria Luz D. Calibayan

    2015-12-01

    Full Text Available This study sought to collect, record, translate, and analyze the Tiruray proverbs. Specifically the study aimed to find out the following: (1 What are the existing proverbs of Tiruray in South Upi, Maguindanao? (2What are the values found as expressed in the proverbs of Tiruraythat have been successfully preserved from their ancestors? The scope of this study was confined to the collected proverbs of Tiruray in South Upi, Maguindanao. The gatheredproverbs were transcribed from Tiruray to Filipino languageand the analysis of values from the translated proverbs was in accordance with the ideas of Andres (1985 and Timbreza (2003. The research design adopted in the study is descriptive content analysis because the main objective of the study was to analyse the values found in Tiruray proverbs. Findings revealed that the Tiruray have rich in oral literary piecessuch as proverbs which are transmitted by word of mouth from generation to generation. The Tiruray proverbs contain differenthuman values that teach or remind people how to live godly lives. Further, Tiruray proverbs conveyed message on how they value peace and harmony in the community for they are peace-loving people. The study of Tiruray proverbs could increase the body of knowledge about the cultural traits of Tiruray who are unique people and have rich cultural heritage.

  13. Assessment of erosion hazard with the USLE and GIS: A case study of the Upper Ewaso Ng'iro North basin of Kenya

    Science.gov (United States)

    Mati, Bancy M.; Morgan, Royston PC; Gichuki, Francis N.; Quinton, John N.; Brewer, Tim R.; Liniger, Hans P.

    The Universal Soil Loss Equation (USLE) was used to predict soil erosion hazard in the Upper Ewaso Ng'iro North basin of Kenya using data from erosion plots and reconnaissance surveys. The R-factor (rainfall erosivity) was determined by extrapolation from the rainfall amount-erosivity relationship, using data from autographic records. The K-factor (soil erodibility) was determined using data obtained by laboratory analysis of soil samples collected from 83 test sites in the basin. The LS-factors (slope length and steepness) were determined from a digital elevation model (DEM) of the basin, while the C-factor (crop and management) was determined from vegetation cover data, obtained from SPOT imagery and field surveys. The P-factor (conservation practice) was estimated from maps of soil conservation, obtained from the Ministry of Agriculture. A raster-based Geographic Information System (GIS) was used to interactively calculate soil loss and map erosion hazard. The results obtained were comparable to measured soil loss values from erosion plots. About 36 percent of the Upper Ewaso Ng'iro basin was predicted to have high risk of erosion, most of this land being in the overgrazed rangelands. Land use and management were the major factors associated with soil erosion, and — to a lesser extent — the topography. Due to the reconnaissance scale of this study there were limitations in determining reliable LS, C and P factors for the USLE. In addition, there is a need for research to determine appropriate P-factors for local soil conservation practices, such as trash lines, fanya-juu terraces and stone lines.

  14. Tungo sa Hardin ng Tao: “Si Bonifacio ang pinakauna, hindi lamang sa Pilipinas, kundi sa buong Indonesia, oo, sa buong Asia na nanggaling sa, at edukado bilang, proletaryado, na nag-organisa ng mga proletaryo.” ni Tan Malaka

    Directory of Open Access Journals (Sweden)

    Ramon Guillermo

    2013-06-01

    Full Text Available Salin ito ng sanaysay na “Ke Taman Manusia” (sinulat taong 1943 nakathang-isip ng Indones na lider komunista na si Tan Malaka o Ibrahimgelar Datuk Tan Malaka (1897-1949 hinggil sa isang “Taman Manusia”o “Hardin ng Tao” na itatayo ng darating na Republika ng Indonesia sahinaharap. Dahil sa futuristiko nitong perspektiba, maihahalintuladang obrang ito ni Tan Malaka sa sanaysay ni Jose Rizal na “Filipinasdentro de cien años” (Ang Pilipinas sa loob ng isang dantaon (1891.Galing ito sa napakalaking akdang pilosopikal ni Tan Malaka napinamagatang Madilog: Materialisme, Dialektika, Logika (1948(Larawan 1 at tila tumatayo bilang pansarang mga repleksyon nito(Magnis-Suseno, 2005, pp. 205-233. Ang akdang ito ay unanginilathala noong 1951 ng Terbitan Widjaya sa Jakarta.

  15. Situation analysis of quality of abortion care in the main maternity hospital in Hai Phòng, Viet Nam

    DEFF Research Database (Denmark)

    Nguyen, My H Ng; Gammeltoft, Tine; Rasch, Vibeke

    2007-01-01

    cultural values influenced quality of care. A quantitative and qualitative approach was employed: a three-part structured survey with 748 women before and after they had an abortion, 20 in-depth interviews with women just after abortion, seven informal interviews with health care staff and 100 participant...... reproductive tract infection and preventing post-abortion infection. To further improve abortion care in hospitals such as Phu-San, training programmes are needed that integrate counselling and clinical skills and address the cultural factors that hinder health staff and women from interacting in an equitable...

  16. Analysis Of The Performance Evaluation Scale For Strıng Instruments In Terms Of Validity And Reliability

    OpenAIRE

    ÇİFTCİ, Ersan; KURTULDU, M KAYHAN

    2010-01-01

    In this study, the performance evaluation scale developed for the string instrument classes was analysed in terms of validity and reliability. The scale, constructed with the help of the evaluation degrees designed by the researchers; was used in evaluating students in final examinations in two different departments by 6 instructors in total. Statistical measurements related to the resulting data were made and reliabilitiy of the scale was tested. In the statistical process, firstly alpha fac...

  17. Tracing Eurosceptic Party Networks via Hyperlink Network Analysis and #FAIL!ng: Can Web Crawlers Keep up with Web Design?

    DEFF Research Database (Denmark)

    Bossetta Jr, Michael Joseph; Dutceac Segesten, Anamaria

    ) Joint Sessions. While attempting to visually map the online hyperlink network of the Sweden Democrats, a Swedish far-right Eurosceptic party, we found the Issuecrawler’s inability to read JavaScript a critical limitation to our study. Knowing that other scholars from a range of disciplines are currently...... programming languages? Or, is its inability to read JavaScript (a limitation shared by all current web crawling software) a reason to fundamentally question its utility as a digital method?...

  18. Sammenhæng mellem fysisk aktivitet i barndom hhv. voksenliv samt holdning til fysisk aktivitet i pædagogisk arbejde

    DEFF Research Database (Denmark)

    Lybæk-Hansen, Esper

    Der er en tæt sammenhæng mellem den pædagogstuderendes fysiske aktivitet i barndommen og fysiske aktivitet i det voksne liv. Den sociale vane (habitus) spiller i denne sammenhæng en afgørende rolle for praksis i det voksne liv! Pædagogstuderende har generelt et positivt forhold til fysisk aktivit...

  19. Novel cost-effective quality control approach for the Cepheid Xpert CT/NG assay for the detection of Chlamydia Trachomatis and Neisseria Gonorrhoeae.

    Science.gov (United States)

    Chan, Maurice; Jiang, Boran; Ng, Siew Yong Lily; Tan, Thean Yen

    2016-06-01

    The Xpert CT/NG is a rapid assay for detection of Neisseria gonorrhoeae and Chlamydia trachomatis. QC materials must be formulated to emulate human specimens, and are prohibitively expensive. A creative, cost-effective QC approach is proposed. The acceptable sample types for the Xpert CT/NG assay were extended to include eye swabs. PMID:27091503

  20. Photo-dynamics of the lyophilized photo-activated adenylate cyclase NgPAC2 from the amoeboflagellate Naegleria gruberi NEG-M strain

    Energy Technology Data Exchange (ETDEWEB)

    Penzkofer, A., E-mail: alfons.penzkofer@physik.uni-regensburg.de [Fakultät für Physik, Universität Regensburg, Universitätsstrasse 31, D-93053 Regensburg (Germany); Tanwar, M.; Veetil, S.K.; Kateriya, S. [Department of Biochemistry, University of Delhi South Campus, Benito Juarez Road, New Delhi 110021 (India); Stierl, M.; Hegemann, P. [Institut für Biologie/Experimentelle Biophysik, Humboldt Universität zu Berlin, Invalidenstrasse 42, D-10115 Berlin (Germany)

    2013-09-23

    Highlights: • Lyophilizing of NgPAC2 from Naegleria gruberi caused loss of BLUF domain activity. • Photo-induced tyrosine to flavin electron transfer in lyophilized NgPAC2. • Photo-induced Tyr–Tyr cross-linking to o,o′-dityrosine in lyophilized NgPAC2. • Photo-induced partial flavin cofactor reduction in lyophilized NgPAC2. • Two NgPAC2 conformations with fast and slow photo-induced electron transfer. - Abstract: The absorption and emission spectroscopic behavior of lyophilized photo-activated adenylate cyclase NgPAC2 from the amoeboflagellate Naegleria gruberi NEG-M strain consisting of a BLUF domain (BLUF = Blue Light sensor Using Flavin) and a cyclase homology domain was studied in the dark, during blue-light exposure and after blue-light exposure at a temperature of 4 °C. The BLUF domain photo-cycle dynamics observed for snap-frozen NgPAC2 was lost by lyophilization (no signaling state formation with flavin absorption red-shift). Instead, blue-light photo-excitation of lyophilized NgPAC2 caused sterically restricted Tyr–Tyr cross-linking (o,o′-ditysosine formation) and partial flavin cofactor reduction.

  1. TESTİNG TWIN DEFICITS HYPHESIS BY BOUND TESTING APPROACH: TURKEY CASE

    Directory of Open Access Journals (Sweden)

    Utku ALTUNÖZ

    2014-06-01

    Full Text Available Twin deficit identity is used to refer to a nation’s current account deficits and a simultaneous fiscal deficit. The term became widely used in the 1980s until the 1990s.Chronic external deficit problems have been encountered since the adoption of the outward-oriented growth model for Turkish economy. This study aim the validity of twin deficits hypothesis (the relationship between the budget deficit and the current account deficit by using the data period 1998:1-2012:3 in Turkey. The Public deficit indicators represented by budget deficit / GNP, reel exchange rate index and government domestic borrowing stock. In addition, current balance deficit is an another indicator for model. For this purpose, bound test approach developed by Pesaran et al.(2001 was applied by using data of determined period. According the result of econometric analysis, there is no long-run relationship between current balance deficit and budget deficit but there is a strong positive relationship between current balance deficit and budget deficit in the short-run.

  2. Revolving fraction collector for preparative capillary gas chromatography in the 100-μg to 1-ng range.

    Science.gov (United States)

    Wassgren, A B; Bergström, G

    1984-11-01

    A gas chromatography fraction collector for use with capillary columns, consisting of an all-glass system with a revolving collector housing six capillary tubes, 100 × 1 mm (internal diameter), has been constructed and tested. The collector is cooled with solid CO2 and has a recovering efficiency of 50-75% in the 100-μg to 1-ng range for compounds of different polarities and volatilities, exemplified by mono- and sesquiter-penes and long-chain esters. The technique has been used for fractionating blends of semiochemicals prior to microchemical reactions, spectroscopic analyses, and behavioral experiments. PMID:24318389

  3. Background identification and suppression for the measurement of (n,g) reactions with the DANCE array at LANSCE

    CERN Document Server

    Reifarth, R; Alpizar-Vicente, A; Browne, J C; Esch, E -I; Greife, U; Haight, R C; Hatarik, R; Kronenberg, A; O'Donnell, J M; Rundberg, R S; Ullmann, J L; Vieira, D J; Wilhelmy, J B; Wouters, J M

    2013-01-01

    In the commissioning phase of the DANCE project (Detector for Advanced Neutron Capture Experiments) measurements have been performed with special emphasis on the identification and suppression of possible backgrounds for the planned (n,g) experiments. This report describes several background sources, observed in the experiment or anticipated from simulations, which will need to be suppressed in this and in similar detectors that are planned at other facilities. First successes are documented in the suppression of background from scattered neutrons captured in the detector as well as from the internal radiation. Experimental results and simulations using the GEANT code are compared.

  4. Structure of Naegleria Tet-like dioxygenase (NgTet1) in complexes with a reaction intermediate 5-hydroxymethylcytosine DNA

    OpenAIRE

    Hashimoto, Hideharu; Pais, June E.; Dai, Nan; Corrêa, Ivan R; Zhang, Xing; Zheng, Yu; Cheng, Xiaodong

    2015-01-01

    The family of ten-eleven translocation (Tet) dioxygenases is widely distributed across the eukaryotic tree of life, from mammals to the amoeboflagellate Naegleria gruberi. Like mammalian Tet proteins, the Naegleria Tet-like protein, NgTet1, acts on 5-methylcytosine (5mC) and generates 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC) in three consecutive, Fe(II)- and α-ketoglutarate-dependent oxidation reactions. The two intermediates, 5hmC and 5fC, could be...

  5. Integratıon Wıthin the EU Accountıng Professıon

    OpenAIRE

    Sevki Celik

    2015-01-01

    Integratıon Wıthin the EU Accountıng Professıon Not to accept globalization in relation to the IFRS ‘good practice being in common practice from the beginning only by multi-national companies, means to stay outside of the advancements uccuring in the world. Our country has become more transparent capital markets, accounting standards for accounting, tax odaklılıktan get rid of IFRS must be appropriate. Accountant is the largest contributor to the increasing competitive environment, to emer...

  6. TEACHER TRAINERS VIEWS OF SERVİCE LEARNİNG COURSE

    Directory of Open Access Journals (Sweden)

    Serdar ARCAGÖK

    2013-06-01

    theoretical and 2 application class hour in a week (M.Ü., 2009. One of the main objectives of education is developing individuals according to social requirements and placing developed individuals to the society (Musolf, 2001: 286. Since teaching occupation is a profession which requires social responsibility, it is thought that CSA course will have reasonable attributions to the students. This study aims to detect the pre-service elementary teachers’ and trainers’ views about CSA course. According to this aim, following questions are tried to be answered; In this study, Community Service Applications in Primary Education Undergraduate Program was evaluated according to the views of pre-service teachers and instructors. İt was used mixed methods in this study. At the first stage of the study, one of the qualitative study methods, “Phenomenology Design” pattern was used to define instructors’ views about CSA in Primary Education Department. At the second stage, to define the pre-service teachers’ views about CSA, a scale was applied to them. The population of the study is composed of the instructors who work in 2010-2011 academic year spring semester in Çanakkale Onsekiz Mart University and the pre-service elemantary teachers who are studying at Çanakkale Onsekiz Mart University during the 2010-2011 academic year. In this study mixed methods was used. Both quantitative and qualitative data were used together; therefore tringulation method was used as data collection method. In the first level of the study, since it was aimed that instructors evaluate CSA by their own expressions, a semi-constructed and nondirective interviewing technique was used. While analyzing data “descriptive analysis” and “content analysis” approaches were used. At the end of the analysis of the data collected at first stage, it was detected that instructors encounter with some problems during CSA course and they consider CSA course important for students’ personal and social

  7. Chronic stress regulates NG2⁺ cell maturation and myelination in the prefrontal cortex through induction of death receptor 6.

    Science.gov (United States)

    Yang, Youjun; Zhang, Yini; Luo, Fei; Li, Baoming

    2016-03-01

    Chronic stress significantly affects neuron morphometry and function in the prefrontal cortex, a brain region controlling cognition and emotion. However, whether and how chronic stress regulates the maturation of NG2-expressing oligodendrocyte precursor cell (NG2(+) cell) and the importance of these changes remained unknown. Here, we report that exposing adult mice to chronic stress results in NG2(+) cell atrophy and myelination arrested in the medial prefrontal cortex (mPFC), and impaired mPFC-dependent functions. These alterations, are phenocopied by overexpression of death receptor 6 (DR6) in NG2(+) cell. Conversely, selectively silencing of DR6 in the NG2(+) cell can partly rescue NG2(+) cell atrophy and cognitive deficiency caused by chronic stress. We further demonstrate that myelination appears necessary for mPFC-dependent cognitive processes, as lysolecithin (LPC)-induced demyelination specifically in the mPFC is sufficient to cause these behavioral and cognitive impairments. Our results indicate that chronic stress impairs cognitive functions, at least in part, through modulation of NG2(+) cell maturation and myelination, and suggest that myelination is require for normal cognitive functions. PMID:26772637

  8. Theoretical prediction of new noble-gas molecules FNgBNR (Ng = Ar, Kr, and Xe; R = H, CH3, CCH, CHCH2, F, and OH).

    Science.gov (United States)

    Chen, Jien-Lian; Yang, Chang-Yu; Lin, Hsiao-Jing; Hu, Wei-Ping

    2013-06-28

    We have computationally predicted a new class of stable noble-gas molecules FNgBNR (Ng = Ar, Kr, Xe; R = H, CH3, CCH, CHCH2, F, and OH). The FNgBNR were found to have compact structures with F-Ng bond lengths of 1.9-2.2 Å and Ng-B bond lengths of ~1.8 Å. The endoergic three-body dissociation energies of FNgBNH to F + Ng + BNH were calculated to be 12.8, 31.7, and 63.9 kcal mol(-1), for Ng = Ar, Kr, and Xe, respectively at the CCSD(T)/CBS level. The energy barriers of the exoergic two-body dissociation to Ng + FBNH were calculated to be 16.1, 24.0, and 33.2 kcal mol(-1) for Ng = Ar, Kr, and Xe, respectively. Our results showed that the dissociation energetics is relatively insensitive to the identities of the terminal R groups. The current study suggested that a wide variety of noble-gas containing molecules with different types of R groups can be thermally stable at low temperature, and the number of potentially stable noble-gas containing molecules would thus increase very significantly. It is expected some of the FNgBNR molecules could be identified in future experiments under cryogenic conditions in noble-gas matrices or in the gas phase.

  9. ng meediaga

    Index Scriptorium Estoniae

    2006-01-01

    Ajakirjanduse ja ettevõtete vahelistest suhetest maine kujundamises. Art. allikaks on Peter Vanderwickeni 2005. aasta jaanuaris Harvard Business Review's ilmunud lugu "Why the news is not the truth". Kommenteerivad Priit Pullerits ja Neeme Raud

  10. NG Kunstikonteiner alustab kirjandusega

    Index Scriptorium Estoniae

    2008-01-01

    Kunstnik Sandra Jõgeva korraldab Tallinnas kultuuritehases Polymer alternatiivgaleriis Non Grata Kunstikonteiner kirjanduslike kohtumiste seeria "Üks hullem kui teine". Kirjandusüritused ei ole mõeldud ainult oma ala insider'itele. 17. juulil esinevad Armin Kõomägi teemal "Kirjandus ja äri", Andrus Elbing "Vangla sinus eneses" jt. Vt. ka Looming, 2008, nr. 8, lk. 1271

  11. Prevalence and Risk Factors of Prostate Cancer in Chinese Men with PSA 4–10 ng/mL Who Underwent TRUS-Guided Prostate Biopsy: The Utilization of PAMD Score

    Science.gov (United States)

    Fang, Dong; Ren, Da; Zhao, Chenglin; Li, Xuesong; Yu, Wei; Wang, Rui; Wang, Huihui; Xi, Chenguang; He, Qun; Wang, Xiaoying; Xin, Zhongcheng; Zhou, Liqun

    2015-01-01

    Purpose. To elucidate the characteristics and risk factors for positive biopsy outcomes in Chinese patients with prostate specific antigen (PSA) 4–10 ng/mL and develop a risk-stratification score model. Methods. The data of 345 patients who underwent transrectal ultrasound-guided prostate biopsy between 2011 and 2013 was retrospectively analyzed. Digital rectal examination (DRE), prostate volume (PV), magnetic resonance imaging (MRI), and smoking status were also collected. Positive biopsy outcomes were defined as prostate cancer (PCa) and high grade PCa (HGPCa, Gleason Score ≥ 7). Results. The median PSA was 7.15 (IQR 5.91–8.45) ng/mL. Overall 138 patients (40.0%) were shown to have PCa, including 100 patients (29.0%) with HGPCa. Smaller PV, elder age, MRI results, and positive DRE were proved to be predictive factors for positive biopsy outcomes in both univariate and multivariate analysis. We developed a “PAMD” score which combined the four factors to categorize patients into three risk groups, and the model performed good predictive sensitivity and specificity. Conclusion. The prevalence of prostate cancer in Chinese patients with PSA 4–10 ng/mL was 40%, including 29% patients with high grade disease. DRE, age, MRI, and PV were predictive factors for positive biopsy outcomes, and the PAMD score model could be utilized for risk-stratification and decision-making. PMID:26557679

  12. Prevalence and Risk Factors of Prostate Cancer in Chinese Men with PSA 4–10 ng/mL Who Underwent TRUS-Guided Prostate Biopsy: The Utilization of PAMD Score

    Directory of Open Access Journals (Sweden)

    Dong Fang

    2015-01-01

    Full Text Available Purpose. To elucidate the characteristics and risk factors for positive biopsy outcomes in Chinese patients with prostate specific antigen (PSA 4–10 ng/mL and develop a risk-stratification score model. Methods. The data of 345 patients who underwent transrectal ultrasound-guided prostate biopsy between 2011 and 2013 was retrospectively analyzed. Digital rectal examination (DRE, prostate volume (PV, magnetic resonance imaging (MRI, and smoking status were also collected. Positive biopsy outcomes were defined as prostate cancer (PCa and high grade PCa (HGPCa, Gleason Score≥7. Results. The median PSA was 7.15 (IQR 5.91–8.45 ng/mL. Overall 138 patients (40.0% were shown to have PCa, including 100 patients (29.0% with HGPCa. Smaller PV, elder age, MRI results, and positive DRE were proved to be predictive factors for positive biopsy outcomes in both univariate and multivariate analysis. We developed a “PAMD” score which combined the four factors to categorize patients into three risk groups, and the model performed good predictive sensitivity and specificity. Conclusion. The prevalence of prostate cancer in Chinese patients with PSA 4–10 ng/mL was 40%, including 29% patients with high grade disease. DRE, age, MRI, and PV were predictive factors for positive biopsy outcomes, and the PAMD score model could be utilized for risk-stratification and decision-making.

  13. Can a Gleason 6 or Less Microfocus of Prostate Cancer in One Biopsy and Prostate-Specific Antigen Level <10 ng/mL Be Defined as the Archetype of Low-Risk Prostate Disease?

    Directory of Open Access Journals (Sweden)

    Gianluigi Taverna

    2012-01-01

    Full Text Available Prostate cancer (PC remains a cause of death worldwide. Here we investigate whether a single microfocus of PC at the biopsy (graded as Gleason 6 or less, ≤5% occupancy and the PSA <10 ng/mL can define the archetype of low-risk prostate disease. 4500 consecutive patients were enrolled. Among them, 134 patients with a single micro-focus of PC were followed up, and the parameters influencing the biochemical relapse (BR were analysed. Out of 134 patients, 94 had clinically significant disease, specifically in 74.26% of the patients with PSA <10 ng/mL. Positive surgical margins and the extracapsular invasion were found in 29.1% and 51.4% patients, respectively. BR was observed in 29.6% of the patients. Cox regression evidenced a correlation between the BR and Gleason grade at the retropubic radical prostatectomy (RRP, capsular invasion, and the presence of positive surgical margins. Multivariate regression analysis showed a statistically significant correlation between the presence of surgical margins at the RRP and BR. Considering a single micro-focus of PC at the biopsy and PSA serum level <10 ng/mL, clinically significant disease was found in 74.26% patients and only positive surgical margins are useful for predicting the BR.

  14. Nonlinear resonance in Duffing oscillator with fixed and integrative time-delayed feedbacks

    Indian Academy of Sciences (India)

    V Ravichandran; V Chinnathambi; S Rajasekar

    2012-03-01

    We study the nonlinear resonance, one of the fundamental phenomena in nonlinear oscillators, in a damped and periodically-driven Duffing oscillator with two types of time-delayed feedbacks, namely, fixed and integrative. Particularly, we analyse the effect of the time-delay parameter and the strength of the time-delayed feedback. Applying the perturbation theory we obtain a nonlinear equation for the amplitude of the periodic response of the system. For a range of values of and , the response amplitude is found to be higher than that of the system in the absence of delayed feedback. The response amplitude is periodic on the parameter with period 2 / where is the angular frequency of the external periodic force. We show the occurrence of multiple branches of the response amplitude curve with and without hysteresis.

  15. Effects of Dăoyĭn Qìgōng in postpolio syndrome patients with cold intolerance

    Directory of Open Access Journals (Sweden)

    Paulo Eduardo Ramos

    2012-09-01

    Full Text Available Postpolio syndrome (PPS is characterized by progressive muscle weakness due to former infection with poliomyelitis and can be associated with other symptoms such as cold intolerance (CI. Dăoyĭn Qìgōng (DQ is a technique in Traditional Chinese Medicine that impacts the circulation of energy and blood. OBJECTIVE: It was to verify the effects of DQ in PPS patients complaining of cold intolerance. METHODS: Ten PPS patients were assessed using the visual analogue scale (VAS adapted for CI before and after intervention with DQ; patients practiced it in a sitting position for 40 minutes, 3 times per week over 3 consecutive months. Patients were reassessed three months after ceasing DQ. RESULTS: There was a statistically significant difference in local and systemic VAS-Cold both at the end of DQ training and three months past the end of this. CONCLUSION: The DQ technique ameliorated CI complaints in patients with PPS.

  16. Návrh kompozitového směrového kormidla pro letoun L 410 NG

    OpenAIRE

    Sokol, Filip

    2015-01-01

    Tato diplomová práce se zabývá návrhem kompozitní konstrukce směrového kormidla letounu L410 NG vycházející ze současné duralově-plátnové konstrukce. Podle předpisu FAR 23 a jeho oběžníků je navržen postup certifikace kompozitní primární konstrukce letounu. Je navržena konstrukce nového směrového kormidla a z vybraných materiálů proveden návrh skladby kompozitních materiálů. Programem MSC Patran je z této konstrukce vytvořen MKP model a pomocí softwarů Compost a Nastran provedena pevnostní ko...

  17. NG2 cells response to axonal alteration in the spinal cord white matter in mice with genetic disruption of neurofilament light subunit expression

    OpenAIRE

    Xiao Zhi; Tang Ya; Wu Ya; Bao Zhen; He Bei

    2008-01-01

    Abstract Background Chondroitin sulphate proteoglycan (NG2) expressing cells, morphologically characterized by multi-branched processes and small cell bodies, are the 4th commonest cell population of non-neuronal cell type in the central nervous system (CNS). They can interact with nodes of Ranvier, receive synaptic input, generate action potential and respond to some pathological stimuli, but the function of the cells is still unclear. We assumed the NG2 cells may play an active role in neur...

  18. Theoretical study of C−H⋯H−B dihydrogen bonded complexes between inert molecules FNgCCH (Ng = Ar and Kr) and borane-amines

    International Nuclear Information System (INIS)

    Graphical abstract: C−H⋯H−B dihydrogen bonding in inert molecules have been explored by studying FNgCCH (Ng = Ar, Kr) complexes with BH3–NH3, BH3–NH2Me and BH3–NHMe2 at MP2/6-311++G(d,p) and MP2/aug-cc-pVDZ level of theories. Further, strength of hydrogen bonding and dihydrogen bonding in inert complexes have been compared under identical condition and it has been found that strength of dihydrogen bonded complexes are ∼15% weaker as compared to hydrogen bonded complexes. Highlights: ► C–H⋯H–B dihydrogen bonding ability of rare gas inserted FNgCCH molecules. ► Comparison of strength of hydrogen bond and dihydrogen bond for inert complexes. ► Dihydrogen bonding is 15% weaker as compare to hydrogen bonding. - Abstract: C−H⋯H−B dihydrogen bonding in inert molecules have been explored by studying FNgCCH (Ng = Ar, Kr) complexes with BH3–NH3, BH3–NH2Me and BH3–NHMe2 at MP2/6-311++G(d,p) and MP2/aug-cc-pVDZ level of theories. The H⋯H contact distances are found to be less than 2.4 Å while binding energies are in the range of 15–18 kJ mol−1. Furthermore, changes in C–H and B–H stretching frequencies authenticate to the formation of C−H⋯H−B dihydrogen bonding in these complexes. Moreover, properties of C−H⋯H−B dihydrogen bonding have also been supplemented by molecular electrostatic potential derived charge, natural population, natural bond order and atoms in molecules analysis. Strength of hydrogen bonding and dihydrogen bonding in inert complexes have been compared under identical condition and it has been found that strength of dihydrogen bonded complexes are ∼15% weaker as compared to hydrogen bonded complexes.

  19. Ultra pressure liquid chromatography-negative electrospray ionization mass spectrometry determination of twelve halobenzoquinones at ng/L levels in drinking water.

    Science.gov (United States)

    Huang, Rongfu; Wang, Wei; Qian, Yichao; Boyd, Jessica M; Zhao, Yuli; Li, Xing-Fang

    2013-05-01

    We report here the characterization of twelve halobenzoquinones (HBQs) using electrospray ionization (ESI) high resolution quadrupole time-of-flight mass spectrometry. The high resolution negative ESI spectra of the twelve HBQs formed two parent ions, [M + H(+) + 2e(-)], and the radical M(-•). The intensities of these two parent ions are dependent on their chemical structures and on instrumental parameters such as the source temperature and flow rate. The characteristic ions of the HBQs were used to develop an ultra pressure liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method. At the UPLC flow rate (400 μL/min) and under the optimized ESI conditions, eleven HBQs showed the stable and abundant transitions [M + H(+) + 2e(-)] → X(-) (X(-) representing Cl(-), Br(-), or I(-)), while dibromo-dimethyl-benzoquinone (DBDMBQ) showed only the transition of M(-•) → Br(-). The UPLC efficiently separates all HBQs including some HBQ isomers, while the MS/MS offers exquisite limits of detection (LODs) at subng/mL levels for all HBQs except DBDMBQ. Combined with solid phase extraction (SPE), the method LOD is down to ng/L. The results from analysis of authentic samples demonstrated that the SPE-UPLC-MS/MS method is reliable, fast, and sensitive for the identification and quantification of the twelve HBQs in drinking water.

  20. 嗜热解烃菌NG80-2的鉴定及其特性%Identification and Characters of Thermophilic Desmolysing Bacteria NG80-2

    Institute of Scientific and Technical Information of China (English)

    唐赟; 冯露; 刘沐之; 马挺; 梁凤来; 刘如林

    2006-01-01

    利用细胞形态、生理生化特征、不同碳源发酵产酸试验及16S rDNA的进化树分析的方法,对分离获得的嗜热解烃细菌NG80-2进行了鉴定,确定为Geobacillus thermodenitrificans,其1 6S rDNA与Geobacillus thermodenitrificans BGSC 94Al的相似性最高,达到99.80%.该菌最适生长温度为65 ℃,最适pH为7.2,能在以原油为唯一碳源的无机盐培养基中生长,并且能选择性降解C15~C36直链烷烃,其中对正-十六烷(C16)的降解率高达58.9%.优良的烃降解性能对微生物开采重质原油和治理石油污染具有潜在的应用价值.

  1. Neuroimaging of the bilingual brain: evidence and research methodology = Neuroimagem do cérebro bilíngüe: evidências e metodologia de pesquisa

    Directory of Open Access Journals (Sweden)

    Ansaldo, Ana Inés

    2008-01-01

    Full Text Available Atualmente mais da metade da população mundial é bilíngüe, sendo o estudo do processamento lingüístico e de seus correlatos cerebrais em indivíduos bilíngües um tópico relevante e inovador na recente área da neuropsicolingüística. Este artigo tem dois objetivos: 1 discutir alguns aspectos metodológicos relacionados ao desenvolvimento de investigações com técnicas de neuroimagem sobre o processamento da linguagem no cérebro bilíngüe e 2 revisar evidências de neuroimagem sobre o processamento lingüístico considerando fatores individuais que têm caracterizado a literatura acerca do bilingüismo, por seu importante papel na representação cerebral bilíngüe da linguagem. Muitos estudos têm mostrado dados sobre a relação entre idade de aquisição, nível de proficiência, idade cronológica, uso de linguagem e forma de exposição às línguas na organização da linguagem no cérebro bilíngüe. A grande variabilidade de métodos de investigação e um controle metodológico nem sempre criterioso tem tornado difícil a generalização de conclusões sobre uma circuitaria cerebral da linguagem em bilíngües. A presente discussão deve contribuir para o debate dos fundamentos teóricos e metodológicos da organização neurofuncional da linguagem no cérebro bilíngüe

  2. Combined Mapping of Multiple clUsteriNg ALgorithms (COMMUNAL): A Robust Method for Selection of Cluster Number, K.

    Science.gov (United States)

    Sweeney, Timothy E; Chen, Albert C; Gevaert, Olivier

    2015-11-19

    In order to discover new subsets (clusters) of a data set, researchers often use algorithms that perform unsupervised clustering, namely, the algorithmic separation of a dataset into some number of distinct clusters. Deciding whether a particular separation (or number of clusters, K) is correct is a sort of 'dark art', with multiple techniques available for assessing the validity of unsupervised clustering algorithms. Here, we present a new technique for unsupervised clustering that uses multiple clustering algorithms, multiple validity metrics, and progressively bigger subsets of the data to produce an intuitive 3D map of cluster stability that can help determine the optimal number of clusters in a data set, a technique we call COmbined Mapping of Multiple clUsteriNg ALgorithms (COMMUNAL). COMMUNAL locally optimizes algorithms and validity measures for the data being used. We show its application to simulated data with a known K, and then apply this technique to several well-known cancer gene expression datasets, showing that COMMUNAL provides new insights into clustering behavior and stability in all tested cases. COMMUNAL is shown to be a useful tool for determining K in complex biological datasets, and is freely available as a package for R.

  3. Myocilin is involved in NgR1/Lingo-1-mediated oligodendrocyte differentiation and myelination of the optic nerve.

    Science.gov (United States)

    Kwon, Heung Sun; Nakaya, Naoki; Abu-Asab, Mones; Kim, Hong Sug; Tomarev, Stanislav I

    2014-04-16

    Myocilin is a secreted glycoprotein that belongs to a family of olfactomedin domain-containing proteins. Although myocilin is detected in several ocular and nonocular tissues, the only reported human pathology related to mutations in the MYOCILIN gene is primary open-angle glaucoma. Functions of myocilin are poorly understood. Here we demonstrate that myocilin is a mediator of oligodendrocyte differentiation and is involved in the myelination of the optic nerve in mice. Myocilin is expressed and secreted by optic nerve astrocytes. Differentiation of optic nerve oligodendrocytes is delayed in Myocilin-null mice. Optic nerves of Myocilin-null mice contain reduced levels of several myelin-associated proteins including myelin basic protein, myelin proteolipid protein, and 2'3'-cyclic nucleotide 3'-phosphodiesterase compared with those of wild-type littermates. This leads to reduced myelin sheath thickness of optic nerve axons in Myocilin-null mice compared with wild-type littermates, and this difference is more pronounced at early postnatal stages compared with adult mice. Myocilin also affects differentiation of oligodendrocyte precursors in vitro. Its addition to primary cultures of differentiating oligodendrocyte precursors increases levels of tested markers of oligodendrocyte differentiation and stimulates elongation of oligodendrocyte processes. Myocilin stimulation of oligodendrocyte differentiation occurs through the NgR1/Lingo-1 receptor complex. Myocilin physically interacts with Lingo-1 and may be considered as a Lingo-1 ligand. Myocilin-induced elongation of oligodendrocyte processes may be mediated by activation of FYN and suppression of RhoA GTPase. PMID:24741044

  4. NASA SIMPL and AVIRIS-NG Coordinated Campaign in Support of ICESat-2; July-August, 2015, Thule, Greenland

    Science.gov (United States)

    Brunt, K. M.; Neumann, T.; Harding, D. J.; Green, R. O.; Markus, T.

    2015-12-01

    Ice, Cloud, and land Elevation Satellite-2 (ICESat-2) is a NASA mission scheduled to launch in 2017 and will carry the Advanced Topographic Laser Altimeter System (ATLAS), which is a photon-counting laser altimeter and represents a new approach to space-borne determination of surface elevation. In support of ICESat-2, NASA has deployed a series of airborne campaigns required for pre-launch satellite algorithm development. The Slope Imaging Multi-polarization Photon-Counting Lidar (SIMPL) and Airborne Visible-Infrared Imaging Spectrometer - Next Generation (AVIRIS-NG) were deployed to Thule, Greenland (July-August, 2015) with 2 science goals: 1) to assess how the lidar surface return, for both sea ice and the ice sheet, is effected in melting conditions; and 2) to investigate how variations in snow grain size effect the lidar surface return over the ice sheet. Each instrument was flown on a separate King Air B-200 in a coordinated airborne mission. This presentation will provide an overview for the campaign and the resultant data products.

  5. The critical role of partially exposed N-terminal valine residue in stabilizing GH10 xylanase from Bacillus sp.NG-27 under poly-extreme conditions.

    Directory of Open Access Journals (Sweden)

    Amit Bhardwaj

    Full Text Available BACKGROUND: Understanding the mechanisms that govern protein stability under poly-extreme conditions continues to be a major challenge. Xylanase (BSX from Bacillus sp. NG-27, which has a TIM-barrel structure, shows optimum activity at high temperature and alkaline pH, and is resistant to denaturation by SDS and degradation by proteinase K. A comparative circular dichroism analysis was performed on native BSX and a recombinant BSX (R-BSX with just one additional methionine resulting from the start codon. The results of this analysis revealed the role of the partially exposed N-terminus in the unfolding of BSX in response to an increase in temperature. METHODOLOGY: We investigated the poly-extremophilicity of BSX to deduce the structural features responsible for its stability under one set of conditions, in order to gain information about its stability in other extreme conditions. To systematically address the role of the partially exposed N-terminus in BSX stability, a series of mutants was generated in which the first hydrophobic residue, valine (Val1, was either deleted or substituted with various amino acids. Each mutant was subsequently analyzed for its thermal, SDS and proteinase K stability in comparison to native BSX. CONCLUSIONS: A single conversion of Val1 to glycine (Gly changed R-BSX from being thermo- and alkali- stable and proteinase K and SDS resistant, to being thermolabile and proteinase K-, alkali- and SDS- sensitive. This result provided insight into the structure-function relationships of BSX under poly-extreme conditions. Molecular, biochemical and structural data revealed that the poly-extremophilicity of BSX is governed by a partially exposed N-terminus through hydrophobic interactions. Such hitherto unidentified N-terminal hydrophobic interactions may play a similar role in other proteins, especially those with TIM-barrel structures. The results of the present study are therefore of major significance for protein folding

  6. The Escape of Sisyphus or What “Post NG-PON2” Should Do Apart from Neverending Capacity Upgrades

    Directory of Open Access Journals (Sweden)

    Martin Maier

    2014-03-01

    Full Text Available The primary design goal of (revolutionary NG-PON1&2 was the provisioning of an ever increasing capacity to cope with video-dominated traffic and handle the explosion of mobile data traffic by means of offloading. Recently, however, questions on the future of “post NG-PON2” have surfaced whether to shift its research focus to business and operation related aspects and move access technology into a substantially different direction than continued capacity upgrades. In fact, recent studies indicate that ultimately the major factor limiting the performance of 4G mobile networks is latency rather than capacity of the backhaul. In this paper, we review recently proposed low-latency techniques for NG-PONs that require architectural modifications at the remote node or distribution fiber level and highlight advanced network coding and real-time polling based low-latency techniques that can be implemented in software, enable NG-PONs to carry higher traffic loads and thereby extend their lifetime, and maintain the passive nature of existent optical distribution networks. Furthermore, we elaborate on emerging trends and open challenges for future post NG-PON2 research. To better understand their true potential, we put them into a wider non-technical and historical perspective leading up to a sustainable Third Industrial Revolution (TIR economy and its underlying Energy Internet.

  7. Photo-dynamics of the lyophilized photo-activated adenylate cyclase NgPAC2 from the amoeboflagellate Naegleria gruberi NEG-M strain

    Science.gov (United States)

    Penzkofer, A.; Tanwar, M.; Veetil, S. K.; Kateriya, S.; Stierl, M.; Hegemann, P.

    2013-09-01

    The absorption and emission spectroscopic behavior of lyophilized photo-activated adenylate cyclase NgPAC2 from the amoeboflagellate Naegleria gruberi NEG-M strain consisting of a BLUF domain (BLUF = Blue Light sensor Using Flavin) and a cyclase homology domain was studied in the dark, during blue-light exposure and after blue-light exposure at a temperature of 4 °C. The BLUF domain photo-cycle dynamics observed for snap-frozen NgPAC2 was lost by lyophilization (no signaling state formation with flavin absorption red-shift). Instead, blue-light photo-excitation of lyophilized NgPAC2 caused sterically restricted Tyr-Tyr cross-linking (o,o‧-ditysosine formation) and partial flavin cofactor reduction.

  8. Nearly 1000 Protein Identifications from 50 ng of Xenopus laevis Zygote Homogenate Using Online Sample Preparation on a Strong Cation Exchange Monolith Based Microreactor Coupled with Capillary Zone Electrophoresis.

    Science.gov (United States)

    Zhang, Zhenbin; Sun, Liangliang; Zhu, Guijie; Cox, Olivia F; Huber, Paul W; Dovichi, Norman J

    2016-01-01

    A sulfonate-silica hybrid strong cation exchange monolith microreactor was synthesized and coupled to a linear polyacrylamide coated capillary for online sample preparation and capillary zone electrophoresis-tandem mass spectrometry (CZE-MS/MS) bottom-up proteomic analysis. The protein sample was loaded onto the microreactor in an acidic buffer. After online reduction, alkylation, and digestion with trypsin, the digests were eluted with 200 mM ammonium bicarbonate at pH 8.2 for CZE-MS/MS analysis using 1 M acetic acid as the background electrolyte. This combination of basic elution and acidic background electrolytes results in both sample stacking and formation of a dynamic pH junction. 369 protein groups and 1274 peptides were identified from 50 ng of Xenopus laevis zygote homogenate, which is comparable with an offline sample preparation method, but the time required for sample preparation was decreased from over 24 h to less than 40 min. Dramatically improved performance was produced by coupling the reactor to a longer separation capillary (∼100 cm) and a Q Exactive HF mass spectrometer. 975 protein groups and 3749 peptides were identified from 50 ng of Xenopus protein using the online sample preparation method. PMID:26670623

  9. Rapid measurement of perchlorate in polar ice cores down to sub-ng L(-1) levels without pre-concentration.

    Science.gov (United States)

    Peterson, Kari; Cole-Dai, Jihong; Brandis, Derek; Cox, Thomas; Splett, Scott

    2015-10-01

    An ion chromatography-electrospray ionization-tandem mass spectrometry (IC-ESI-MS/MS) method has been developed for rapid and accurate measurement of perchlorate in polar snow and ice core samples in which perchlorate concentrations are expected to be as low as 0.1 ng L(-1). Separation of perchlorate from major inorganic species in snow is achieved with an ion chromatography system interfaced to an AB SCIEX triple quadrupole mass spectrometer operating in multiple reaction monitoring mode. Under optimized conditions, the limit of detection and lower limit of quantification without pre-concentration have been determined to be 0.1 and 0.3 ng L(-1), respectively, with a linear dynamic range of 0.3-10.0 ng L(-1) in routine measurement. These represent improvements over previously reported methods using similar analytical techniques. The improved method allows fast, accurate, and reproducible perchlorate quantification down to the sub-ng L(-1) level and will facilitate perchlorate measurement in the study of natural perchlorate production with polar ice cores in which perchlorate concentrations are anticipated to vary in the low and sub-ng L(-1) range. Initial measurements of perchlorate in ice core samples from central Greenland show that typical perchlorate concentrations in snow dated prior to the Industrial Revolution are about 0.8 ng L(-1), while perchlorate concentrations are significantly higher in recent (post-1980) snow, suggesting that anthropogenic sources are a significant contributor to perchlorate in the current environment. PMID:26297465

  10. Determination of eight nitrosamines in water at the ng L(-1) levels by liquid chromatography coupled to atmospheric pressure chemical ionization tandem mass spectrometry.

    Science.gov (United States)

    Ripollés, Cristina; Pitarch, Elena; Sancho, Juan V; López, Francisco J; Hernández, Félix

    2011-09-19

    In this work, we have developed a sensitive method for detection and quantification of eight N-nitrosamines, N-nitrosodimethylamine (NDMA), N-nitrosomorpholine (NMor), N-nitrosomethylethylamine (NMEA), N-nitrosopirrolidine (NPyr), N-nitrosodiethylamine (NDEA), N-nitrosopiperidine (NPip), N-nitroso-n-dipropylamine (NDPA) and N-nitrosodi-n-butylamine (NDBA) in drinking water. The method is based on liquid chromatography coupled to tandem mass spectrometry, using atmospheric pressure chemical ionization (APCI) in positive mode with a triple quadrupole analyzer (QqQ). The simultaneous acquisition of two MS/MS transitions in selected reaction monitoring mode (SRM) for each compound, together with the evaluation of their relative intensity, allowed the simultaneous quantification and reliable identification in water at ppt levels. Empirical formula of the product ions selected was confirmed by UHPLC-(Q)TOF MS accurate mass measurements from reference standards. Prior to LC-MS/MS QqQ analysis, a preconcentration step by off-line SPE using coconut charcoal EPA 521 cartridges (by passing 500 mL of water sample) was necessary to improve the sensitivity and to meet regulation requirements. For accurate quantification, two isotope labelled nitrosamines (NDMA-d(6) and NDPA-d(14)) were added as surrogate internal standards to the samples. The optimized method was validated at two concentration levels (10 and 100 ng L(-1)) in drinking water samples, obtaining satisfactory recoveries (between 90 and 120%) and precision (RSDwater samples: chlorinated from drinking water and wastewater treatment plants (DWTP and WWTP, respectively), wastewaters subjected to ozonation and tap waters.

  11. Impacts of agricultural intensification through upscaling of suitable rainwater harvesting technologies in the upper Ewaso Ng'iro North basin, Kenya

    Directory of Open Access Journals (Sweden)

    J. K. Mutiga

    2011-03-01

    Full Text Available Changes in land cover and land use can lead to significant impacts to hydrology by affecting the amount of runoff, soil moisture and groundwater recharge over a range of temporal and spatial scales. However, hydrologic effects of these changes are still an unknown at watershed scale. Moreover, predicting the effects of land cover/use and climate change on hydrological cycle has remained a major challenge. This is because of the complexity and uncertainty of future climate changes making it difficult to predict the consequences. It is against this backdrop that, for sustainable water resources management, assessment of the impacts of land cover/use change on hydrological regime at all scales becomes critical. During this study, we applied the SWAT model to assess the impacts of area hydrology between baseline and alternative scenario (upscaling of rainwater harvesting technologies. Specifically, our overall objective was to quantitatively evaluate the effects of land use changes on watershed hydrology in the upper Ewaso Ng'iro North basin in Kenya. This was achieved by estimating hydrological responses under historical land use scenarios obtained from the multi-temporal satellite imageries of 1987, 1995 and 2003. The model performance was found to be relatively good (Nash and Sutcliffe efficient of 70%. Stream flow analysis was carried out for different parts of the basin to understand its hydrological responses, especially, the behavior of base flow. The results show a decrease in base flow during 1987–2003 period with decreasing forest, bush and grass covers, which can be attributed to poor natural vegetation emanating mainly from overgrazing and deforestation for agricultural activities. In conclusion, the study clearly shows that, assessment of hydrologic effects of land use changes is critical for a sustainable water resources planning and management of the basin.

  12. Experimental Demonstration of 6-Mode Division Multiplexed NG-PON2: Cost Effective 40 Gbit/s/Spatial-Mode Access Based on 3D Laser Inscribed Photonic Lanterns

    DEFF Research Database (Denmark)

    Asif, Rameez; Hu, Hao; Mitchell, Paul;

    We report the first space-division-multiplexed based symmetric NG-PON2 network by effi- ciently transmitting 40 Gbit/s/spatial-mode. Error free transmission (BER of 10−9 ) is obtained for all the downstream and upstream data tributaries over 1-km 6-spatial-mode FMF without using MIMO DSP......We report the first space-division-multiplexed based symmetric NG-PON2 network by effi- ciently transmitting 40 Gbit/s/spatial-mode. Error free transmission (BER of 10−9 ) is obtained for all the downstream and upstream data tributaries over 1-km 6-spatial-mode FMF without using MIMO DSP...

  13. Förbättrad produktionsplanering med SAP APO : En studie på Siemens Industrial Turbomachinery AB i Finspång

    OpenAIRE

    Walldén, Joel; Elofsson Tottmar, Kristian

    2010-01-01

    Siemens Industrial Turbomachinery AB, nedan utskrivet som Siemens, är en svensk turbintillverkare placerad i Finspång som tillhör den tyska koncernen Siemens AG. Turbintillverkningen i Finspång började 1911 under namnet STAL men har sedan dess bytt ägarstruktur flertalet gånger. STAL-Laval, ABB STAL och Alstom Power är ett urval av de namn verksamheten har haft innan den blev en del av Siemens. Ett problem som Siemens står inför vid produktionsplanering av gasturbiner är de mycket långa ledti...

  14. Preparation and Study of Charge Transfer Complexes of N.N.N.N-Tetra-Methyl-Para-Phenylene-Diamine (TMPD with NG, Tetryl, RDX and HMX

    Directory of Open Access Journals (Sweden)

    K. Bahadur

    1983-04-01

    Full Text Available Charge transfer complexes of NG tetryl, RDX and HMX as electron acceptors with tetra-methyl-para-phenylene-diamine (TMPD as electron donor were prepared and studied. When the solution of nitro-explosives in an inert solvent were added to a solution of TMPD, an intense violet colour was produced. This colour is due to the formation of TMPD cation known as Wurster radical formed from TMPD through the loss of an electron. It has been shown that one mole of each of NG, tetryl, RDX and HMX is needed to form oneTMPD cation. These complexes have also been studied by ultra-violet and infrared spectroscopy.

  15. The Escape of Sisyphus or What “Post NG-PON2” Should Do Apart from Neverending Capacity Upgrades

    OpenAIRE

    Martin Maier

    2014-01-01

    The primary design goal of (r)evolutionary NG-PON1&2 was the provisioning of an ever increasing capacity to cope with video-dominated traffic and handle the explosion of mobile data traffic by means of offloading. Recently, however, questions on the future of “post NG-PON2” have surfaced whether to shift its research focus to business and operation related aspects and move access technology into a substantially different direction than continued capacity upgrades. In fact, recent studies ...

  16. Effects of cerebrolysin on the proliferation of NG2 proteoglycan-pesitive neural progenitor cells%脑蛋白水解物注射液对NG2蛋白聚糖阳性神经祖细胞增殖的影响

    Institute of Scientific and Technical Information of China (English)

    于秀军; 楯林義孝; 郭力

    2009-01-01

    目的 探讨脑蛋白水解物注射液(Cerebrolysin,CL)对NG2蛋白聚糖阳性神经祖细胞(NG2细胞)增殖的作用.方法 根据文献从成年大鼠海马原代及传代培养NG2细胞,以免疫荧光染色法鉴定细胞性质,以乳酸脱氢酶(LDH)分析法测定细胞活性,以原位缺口末端标记技术(即TUNEL法)观察细胞凋亡,5-溴脱氧尿嘧啶核苷(BrdU)掺入法鉴定新生细胞.结果 CL处置明显增加NG2细胞数,并显著减少TUNEL阳性细胞数.但对BrdU阳性细胞数无明显影响.结论 CL能抑制NG2细胞凋亡,从而促进NG2细胞的增殖.

  17. Comparison of the effects of NG-nitro-L-arginine and indomethacin on the hypercapnic cerebral blood flow increase in rats

    DEFF Research Database (Denmark)

    Wang, Qian; Pelligrino, D A; Paulson, O B;

    1994-01-01

    The effects of NG-nitro-L-arginine (NOLAG), an inhibitor of nitric oxide synthase (NOS), and of indomethacin, an inhibitor of cyclooxygenase, on the rise in cerebral blood flow (CBF) accompanying increasing levels of hypercapnia (paCO2 = 40-135 mmHg) were studied in anesthetized rats. CBF...

  18. Effect of nitric oxide blockade by NG-nitro-L-arginine on cerebral blood flow response to changes in carbon dioxide tension

    DEFF Research Database (Denmark)

    Wang, Qian; Paulson, O B; Lassen, N A

    1992-01-01

    The importance of nitric oxide (NO) for CBF variations associated with arterial carbon dioxide changes was investigated in halothane-anesthetized rats by using an inhibitor of nitric oxide synthase, NG-nitro-L-arginine (NOLAG). CBF was measured by intracarotid injection of 133Xe. In normocapnia...

  19. NG-PON2 Architecture Enabled by Heterogeneous Space Division Multiplexing with Distributed Light Source: A Proof-of-Concept Evaluation

    DEFF Research Database (Denmark)

    Asif, Rameez; Imran, Muhammad; Ye, Feihong;

    2015-01-01

    We have numerically evaluated the next generation passive optical network stage-2 (NG-PON2) architecture based on heterogeneous space division multiplexing (SDM) and wavelength-division multiplexing (WDM). Error free transmission (BER of 10−9 ) is obtained for all the downstream (DS) and upstream...

  20. On the Least-Squares Fitting of Slater-Type Orbitals with Gaussians: Reproduction of the STO-NG Fits Using Microsoft Excel and Maple

    Science.gov (United States)

    Pye, Cory C.; Mercer, Colin J.

    2012-01-01

    The symbolic algebra program Maple and the spreadsheet Microsoft Excel were used in an attempt to reproduce the Gaussian fits to a Slater-type orbital, required to construct the popular STO-NG basis sets. The successes and pitfalls encountered in such an approach are chronicled. (Contains 1 table and 3 figures.)

  1. Evaluation of the Prostate Imaging Reporting and Data System for Magnetic Resonance Imaging Diagnosis of Prostate Cancer in Patients with Prostate-specific Antigen <20 ng/ml

    Directory of Open Access Journals (Sweden)

    Xuan Wang

    2016-01-01

    Conclusions: The PI-RADS score correlates with the PCa detection rate in patients with PSA <20 ng/ml. The summed score of T2WI + DWI has the highest accuracy in detection of PCa. However, the sensitivity should be further improved.

  2. Muutumismäng 2010. 1.-5. osa / tekst Kerttu Soans ; kommenteerinud Tõnis Elling, Maiu Herzmann, Reet Martinson, Marek Sildoja, Pille Mitt, Marko Vilipson

    Index Scriptorium Estoniae

    Soans, Kerttu, 1961-

    2010-01-01

    Ajakirja Üks Muutumismäng 2010 jälgib nende käekäiku, kes soovivad langetada kaalu osaledes spordiklubi Artic Sport XL-treeningprogrammis. Tutvustatakse ka inimest, kes on eeskujuks teistele treenijatele ja kaalulangetajatele

  3. Mild hypothermia combined with a scaffold of NgR-silenced neural stem cells/Schwann cells to treat spinal cord injur y

    Institute of Scientific and Technical Information of China (English)

    Dong Wang; Jinhua Liang; Jianjun Zhang; Shuhong Liu; Wenwen Sun

    2014-01-01

    Because the inhibition of Nogo proteins can promote neurite growth and nerve cell differenti-ation, a cell-scaffold complex seeded with Nogo receptor (NgR)-silenced neural stem cells and Schwann cells may be able to improve the microenvironment for spinal cord injury repair. Previ-ous studies have found that mild hypothermia helps to attenuate secondary damage in the spinal cord and exerts a neuroprotective effect. Here, we constructed a cell-scaffold complex consisting of a poly(D,L-lactide-co-glycolic acid) (PLGA) scaffold seeded with NgR-silenced neural stem cells and Schwann cells, and determined the effects of mild hypothermia combined with the cell-scaffold complexes on the spinal cord hemi-transection injury in the T9 segment in rats. Compared with the PLGA group and the NgR-silencing cells+PLGA group, hindlimb motor function and nerve electrophysiological function were clearly improved, pathological changes in the injured spinal cord were attenuated, and the number of surviving cells and nerve ifbers were increased in the group treated with the NgR-silenced cell scaffold+mild hypothermia at 34°C for 6 hours. Furthermore, fewer pathological changes to the injured spinal cord and more surviv-ing cells and nerve ifbers were found after mild hypothermia therapy than in injuries not treated with mild hypothermia. These experimental results indicate that mild hypothermia combined with NgR gene-silenced cells in a PLGA scaffold may be an effective therapy for treating spinal cord injury.

  4. NG2 cells response to axonal alteration in the spinal cord white matter in mice with genetic disruption of neurofilament light subunit expression

    Directory of Open Access Journals (Sweden)

    Xiao Zhi

    2008-10-01

    Full Text Available Abstract Background Chondroitin sulphate proteoglycan (NG2 expressing cells, morphologically characterized by multi-branched processes and small cell bodies, are the 4th commonest cell population of non-neuronal cell type in the central nervous system (CNS. They can interact with nodes of Ranvier, receive synaptic input, generate action potential and respond to some pathological stimuli, but the function of the cells is still unclear. We assumed the NG2 cells may play an active role in neuropathogenesis and aimed to determine if NG2 cells could sense and response to the alterations in the axonal contents caused by disruption of neurofilament light subunit (NFL expression. Results In the early neuropathological development stage, our study showed that the diameter of axons of upper motor neurons of NFL-/- mice decreased significantly while the thickness of their myelin sheath increased remarkably. Although there was an obvious morphological distortion in axons with occasionally partial demyelination, no obvious changes in expression of myelin proteins was detected. Parallel to these changes in the axons and their myelination, the processes of NG2 cells were disconnected from the nodes of Ranvier and extended further, suggesting that these cells in the spinal cord white matter could sense the alteration in axonal contents caused by disruption of NFL expression before astrocytic and microglial activation. Conclusion The structural configuration determined by the NFL gene may be important for maintenance of normal morphology of myelinated axons. The NG2 cells might serve as an early sensor for the delivery of information from impaired neurons to the local environment.

  5. NG2细胞在大鼠脊髓压迫性损伤急性期内源性增殖及形态变化规律%Endogenous proliferation and morphological characteristics of NG2-cells in compressed spinal cord injury of rats

    Institute of Scientific and Technical Information of China (English)

    漆伟; 孙善全; 冉建华; 黄思琴; 伍修宇; 侯良绢; 卓飞; 陈臻

    2013-01-01

    目的 研究NG2细胞在大鼠脊髓损伤白质内源性增殖及形态特征.方法 成年SD雄性大鼠42只,随机平均分为模型组和假手术组.按课题组自行设计的方法制作脊髓压迫模型,假手术组仅暴露脊髓.分别于术后1、3、7d运用免疫组化检测脊髓内NG2细胞的表达.采用Image Pro Plus6.0软件对NG2阳性细胞计数并测量其胞体面积和突起长度.结果 伤后1d,NG2+细胞增多(30.17±11.08)/视野,至3d达到高峰(90.75±9.40)/视野,7d后下降(78.38±8.91)/视野,但仍多于假手术组(19.92±6.68)/视野(P<0.05).在假手术组,NG2+细胞平均胞体面积为(205.67±10.80)μm2、平均突起长度为(22.92±1.24)μm,伤后1d,NG2+细胞胞体变小(128.25±32.06)μm2、突起变短(10.98±4.25) μm,3d后胞体变大(225.26±16.64) μm2、突起增长(18.63±2.26) μm(P< 0.05),至7d变化不明显(P>0.05).在脊髓压迫损伤后,可见许多胞体较小呈圆形、突起少或无的NG2+细胞集落.结论 在脊髓压迫损伤一周内,NG2细胞增殖活跃,胞体渐大,突起变长,但仍短于正常.%Objective To investigate endogenous proliferation and morphological characteristics of NG2-cells in the white matter of compressed spinal cord injury (ACSCI) of rats. Methods 42 adult SD rats were randomly divided into 2 groups: model group (n=21), which underwent spinal cord compression by a self-made device, and sham group (n=21), which underwent only laminectomy. Expression of NG2-positive cells was detected by IHC after 1, 3 and 7 days. The average number, soma area, process length of NG2+-cells were measured by Image Pro Plus6.0. Results Compared with control group (19.92 ±6.68 per field), the number of NG2+-cells increased at 1d (30.17 ±11.08 per field), peaked at 3d (90.75 ±9.40 per field), and decreased at 7d (78.38±8.91 per field) (P0.05). There were many clusters of NG2+ cells that had smaller and round soma, fewer or no processes after CSCI. Conclusion NG2+-cells could

  6. 脑蛋白水解物注射液对NG2蛋白聚糖阳性神经祖细胞分化的影响%Effects of Cerebrolysin on the differentiation of the NG2 proteoglycan-positive neural progenitor cells

    Institute of Scientific and Technical Information of China (English)

    于秀军; 楯林羲孝; 郭力

    2010-01-01

    目的 探讨脑蛋白水解物注射液(Cerebrolysin,CL)对NG2蛋白聚糖阳性神经祖细胞(NG2细胞)分化的作用.方法 根据文献从成年大鼠海马原代培养NG2细胞,7天后添加10μl/ml(0.4mg/ml)CL继续培养7天,以免疫荧光双重染色法鉴定细胞性质.结果 10μl/ml CL处置明显增加NG2细胞中Map2a/b、Synapsin I、VGAD和GABA表达水平.结论 CL促进NG2细胞向神经元(特别是GABA能抑制性中间神经元)分化.

  7. AMIGO3 is an NgR1/p75 co-receptor signalling axon growth inhibition in the acute phase of adult central nervous system injury.

    Directory of Open Access Journals (Sweden)

    Zubair Ahmed

    Full Text Available Axon regeneration in the injured adult CNS is reportedly inhibited by myelin-derived inhibitory molecules, after binding to a receptor complex comprised of the Nogo-66 receptor (NgR1 and two transmembrane co-receptors p75/TROY and LINGO-1. However, the post-injury expression pattern for LINGO-1 is inconsistent with its proposed function. We demonstrated that AMIGO3 levels were significantly higher acutely than those of LINGO-1 in dorsal column lesions and reduced in models of dorsal root ganglion neuron (DRGN axon regeneration. Similarly, AMIGO3 levels were raised in the retina immediately after optic nerve crush, whilst levels were suppressed in regenerating optic nerves, induced by intravitreal peripheral nerve implantation. AMIGO3 interacted functionally with NgR1-p75/TROY in non-neuronal cells and in brain lysates, mediating RhoA activation in response to CNS myelin. Knockdown of AMIGO3 in myelin-inhibited adult primary DRG and retinal cultures promoted disinhibited neurite growth when cells were stimulated with appropriate neurotrophic factors. These findings demonstrate that AMIGO3 substitutes for LINGO-1 in the NgR1-p75/TROY inhibitory signalling complex and suggests that the NgR1-p75/TROY-AMIGO3 receptor complex mediates myelin-induced inhibition of axon growth acutely in the CNS. Thus, antagonizing AMIGO3 rather than LINGO-1 immediately after CNS injury is likely to be a more effective therapeutic strategy for promoting CNS axon regeneration when combined with neurotrophic factor administration.

  8. AMIGO3 is an NgR1/p75 co-receptor signalling axon growth inhibition in the acute phase of adult central nervous system injury.

    Science.gov (United States)

    Ahmed, Zubair; Douglas, Michael R; John, Gabrielle; Berry, Martin; Logan, Ann

    2013-01-01

    Axon regeneration in the injured adult CNS is reportedly inhibited by myelin-derived inhibitory molecules, after binding to a receptor complex comprised of the Nogo-66 receptor (NgR1) and two transmembrane co-receptors p75/TROY and LINGO-1. However, the post-injury expression pattern for LINGO-1 is inconsistent with its proposed function. We demonstrated that AMIGO3 levels were significantly higher acutely than those of LINGO-1 in dorsal column lesions and reduced in models of dorsal root ganglion neuron (DRGN) axon regeneration. Similarly, AMIGO3 levels were raised in the retina immediately after optic nerve crush, whilst levels were suppressed in regenerating optic nerves, induced by intravitreal peripheral nerve implantation. AMIGO3 interacted functionally with NgR1-p75/TROY in non-neuronal cells and in brain lysates, mediating RhoA activation in response to CNS myelin. Knockdown of AMIGO3 in myelin-inhibited adult primary DRG and retinal cultures promoted disinhibited neurite growth when cells were stimulated with appropriate neurotrophic factors. These findings demonstrate that AMIGO3 substitutes for LINGO-1 in the NgR1-p75/TROY inhibitory signalling complex and suggests that the NgR1-p75/TROY-AMIGO3 receptor complex mediates myelin-induced inhibition of axon growth acutely in the CNS. Thus, antagonizing AMIGO3 rather than LINGO-1 immediately after CNS injury is likely to be a more effective therapeutic strategy for promoting CNS axon regeneration when combined with neurotrophic factor administration. PMID:23613963

  9. Survey of traditional beliefs in the Hungarian Csángó and Székely ethnomedicine in Transylvania, Romania

    OpenAIRE

    Nóra Papp; Kata Birkás-Frendl; Tímea Bencsik; Szilvia Stranczinger; Dóra Czégényi

    2014-01-01

    Transylvania, part of Romania, has a long-standing culture of Hungarian ethnomedicinal practices. The aim of this study was to review the unexplored ethnopharmacological use of plants, animals and other materials, focusing mainly on the beliefs surrounding them; and compare them with traditional uses from other countries and with scientific literature. An ethnobotanical inventory was conducted among Csángó and Székely Hungarians in three areas of the country between 2007 and 2012. Questionnai...

  10. IND UCTION OF SPAW NING IN THE TR OPICAL WALKING CATFISH (C la rias b atr ac hus BY CONTROLLI NG WATER L EVEL AND T EMPERAT URE

    Directory of Open Access Journals (Sweden)

    M. ZAIRIN JR.

    2001-01-01

    Full Text Available This experiment was carried out t o confirm the rol e of water level and w ater temperature i n indu cing t he s pawning o f tr opical walking catfi sh . Ma ture mal es an d females reared un de r 23 - 2 5 °C, were paired and in duced to spa wn by cont rolling w ater level and w ater temperature. Decr easi ng water level and returni ng it to its original level r esulted i n a low spawni ng r ate (l es s t han 6. 7 % at 23 °C . Decreasing water level with simultaneous increase in water temperature, followed by returning the respective levels to their originals gave high spa wning rates ( 41.7 — 50 %; whereas the s ame treatment but without a ny water temperature decrea sed when t he wate r le vel was return ed t o t he initial level, gave a lo w spawning rate (16 .7 %. In creasin g water level only, fail ed to ind uce spa wning . A high spawning rate was obtained also when changes in water level were ca rried out under high t emperature of 28 °C. No fish spawned in the ab sence of the environmental stimulation. From the results, i t is confirmed that water le vel and t emperature play important r oles i n i nducin g spaw ning of tropical walki ng catfish. Changes i n water t emperature probably increas e the sensitivity of fish to th e change in water level. Prolo nged exposure to high water tem perature c ould also improve the sensitivity of fish.

  11. Endothelium-dependent relaxations in sheep pulmonary arteries and veins: resistance to block by NG-nitro-L-arginine in pulmonary hypertension.

    OpenAIRE

    Kemp, B K; Smolich, J J; Ritchie, B C; Cocks, T. M.

    1995-01-01

    1. The effect of the nitric oxide synthase inhibitor, NG-nitro-L-arginine (L-NOARG), on endothelium-dependent relaxation to a receptor-independent agent, ionomycin, was examined in isolated pulmonary arteries and veins from control, short-term and chronic pulmonary hypertensive sheep. All vessel segments were contracted to optimal levels of active force with endothelin-1 to record endothelium-dependent relaxation. 2. Pulmonary hypertension was induced by continuous pulmonary artery air emboli...

  12. Multi-analyte approach for the determination of ng.l-1 amounts of steroid hormones in unidentified aqueous samples

    OpenAIRE

    Noppe, H.; VERHEYDEN, K; Gillis, W.; Courtheyn, D.; Vanthemsche, P.; De Brabander, H.F.

    2006-01-01

    Since the 70s, many analytical methods for the detection of illegal growth promotors, such as thyreostats, anabolics, ß-agonists and corticosteroids have been developed for a wide range of matrices of animal origin, including meat, fat, organ tissue, urine and faeces. The aim of this study was to develop an analytical method for the determination of ng.l-1 levels of estrogens, gestagens, androgens (EGAs) and corticosteroids in aqueous preparations (i.e. drinking water, drinking water suppleme...

  13. An NF-κB--EphrinA5-Dependent Communication between NG2(+) Interstitial Cells and Myoblasts Promotes Muscle Growth in Neonates.

    Science.gov (United States)

    Gu, Jin-Mo; Wang, David J; Peterson, Jennifer M; Shintaku, Jonathan; Liyanarachchi, Sandya; Coppola, Vincenzo; Frakes, Ashley E; Kaspar, Brian K; Cornelison, Dawn D; Guttridge, Denis C

    2016-01-25

    Skeletal muscle growth immediately following birth is critical for proper body posture and locomotion. However, compared with embryogenesis and adulthood, the processes regulating the maturation of neonatal muscles is considerably less clear. Studies in the 1960s predicted that neonatal muscle growth results from nuclear accretion of myoblasts preferentially at the tips of myofibers. Remarkably, little information has been added since then to resolve how myoblasts migrate to the ends of fibers. Here, we provide insight into this process by revealing a unique NF-κB-dependent communication between NG2(+) interstitial cells and myoblasts. NF-κB in NG2(+) cells promotes myoblast migration to the tips of myofibers through cell-cell contact. This occurs through expression of ephrinA5 from NG2(+) cells, which we further deduce is an NF-κB target gene. Together, these results suggest that NF-κB plays an important role in the development of newborn muscles to ensure proper myoblast migration for fiber growth.

  14. NG2-expressing glial precursor cells are a new potential oligodendroglioma cell initiating population in N-ethyl-N-nitrosourea-induced gliomagenesis.

    Science.gov (United States)

    Briançon-Marjollet, Anne; Balenci, Laurent; Fernandez, Manuel; Estève, François; Honnorat, Jérôme; Farion, Régine; Beaumont, Marine; Barbier, Emmanuel; Rémy, Chantal; Baudier, Jacques

    2010-10-01

    Gliomas are the most common primary brain tumor affecting human adults and remain a therapeutic challenge because cells of origin are still unknown. Here, we investigated the cellular origin of low-grade gliomas in a rat model based on transplacental exposure to N-ethyl-N-nitrosourea (ENU). Longitudinal magnetic resonance imaging coupled to immunohistological and immunocytochemical analyses were used to further characterize low-grade rat gliomas at different stages of evolution. We showed that early low-grade gliomas have characteristics of oligodendroglioma-like tumors and exclusively contain NG2-expressing slow dividing precursor cells, which express early markers of oligodendroglial lineage. These tumor-derived precursors failed to fully differentiate into oligodendrocytes and exhibited multipotential abilities in vitro. Moreover, a few glioma NG2+ cells are resistant to radiotherapy and may be responsible for tumor recurrence, frequently observed in humans. Overall, these findings suggest that transformed multipotent NG2 glial precursor cell may be a potential cell of origin in the genesis of rat ENU-induced oligodendroglioma-like tumors. This work may open up new perspectives for understanding biology of human gliomas.

  15. Strategic environmental evaluation of natural gas - SEA/NG: a management tool for development of a environmental conservation policy; Avaliacao ambiental estrategica para o gas natural - AAE/GN: instrumento de gestao do desenvolvimento para uma politica de conservacao ambiental

    Energy Technology Data Exchange (ETDEWEB)

    Comar, Vito; Turdera, Mirko Valenzuela; Silva, Paulo Souza; Benedetti Filho, Edemar; Santiago, Etenaldo Felipe; Costa, Fabio Edir dos Santos [Universidade Estadual do Mato Grosso do Sul (UEMS), Campo Grande, MS (Brazil). Centro de Analise e Monitoramento Ambiental do Gas Natural (GASLAB); Moretti, Edvaldo; Oliveira, Jorge Eremites de; Sciamarelli, Alan; Freitas Filho, Jose Daniel de; Vieira, Maria Helena Pereira [Universidade Estadual do Mato Grosso do Sul (UEMS), Campo Grande, MS (Brazil); Soares, Afranio Jose Soriano [UNESP, Rio Claro, SP (Brazil); Oliveira, Henrique de; Comunello, Eder [EMBRAPA, Dourados, MS (Brazil). Centro de Pesquisas Agropecuaria do Oeste (CPAO); Freire, Luiz G.M. [PETROBRAS, Rio de Janeiro, RJ (Brazil)

    2004-07-01

    The methodology for a Strategic Environmental Evaluation of Natural Gas - SEA/NG incorporates within the analysis of the project the socio-cultural, economic, energetic and environmental implications of the transformations, due to the adoption of natural gas, in the area of influence of gas pipelines and gas-powered thermo-electric plants. It was based on the case study of a gas pipeline planned to reach the city of Dourados from Campo Grande, capital of the Brazilian State of Mato Grosso do Sul, which implied the construction of a thermoelectric plant in Dourados. The SEA/NG: 1. Defines the best alternative track for gas pipelines; 2. Proposes location alternatives for gas-powered thermo-electric plants; 3. Evaluates the cumulative and synergic effects of human activities in the socio-environmental aspects; 4. Studies the dynamics of regional development; 4. Supports urban planning decisions in cities housing gas-powered thermo-electric plants; 5. Proposes a System of Analysis and Environmental Monitoring for Natural Gas. Qualitative-quantitative matrixes were used to evaluate the cumulative and synergic effects of the adoption of natural gas within the regional energy matrix, determine the best alternative track for the gas pipeline proposed from Campo Grande to Dourados, and to identify the best location for a gas-powered thermo-electric plant. The matrixes integrated the areas of geology, hydrology, ecology and socio-economics issues both in the phases of construction and operation of the pipeline. Diagrams and interactive conceptual models were also used to synthesize the principal components involved in the choice of the best gas pipeline alternative track. This project received financial grants from PETROBRAS, MSGAS, TBG and FINEP. (author)

  16. F actors A ff ecti ng the Adoption of Internet Banking Amongst IIUM’ students : A Structural Equation Modeling Approach (SEM

    Directory of Open Access Journals (Sweden)

    NABIL HUSSEIN AL - FAHIM

    2012-12-01

    Full Text Available This article examines the factors that determine the internet banking adoptio n amongst International Islamic University Malaysia (IIUM and its causal effects using a theoretical model based on the Technology A cceptance Model (TAM. The research model consists of four exogenous latent constructs, namely, awareness , perceived usefulness, trust and perceived risk and endogenous latent construct namely Internet banking adoption . Data relating to constructs were collected from 200 university’s students in (IIUM and subjected to structural Equation Modeling (SEM analysis. Confirmatory Factor Analysis (CFA was performed to examine the reliability, construct validity, convergent validity and goodness of fit of structural models and measurement models. The hypothesized structural model f i ts the data well . The results show that the significant factor that leads to the adoption of internet banking is perceived usefulness but aw arene ss, trust and risk have negative significant towards the use of internet banking.

  17. Creati ng Customer - Based Brand Equity and Measuring Brand Perception of Tourists who Travel for Thermal Tourism: Balcova Survey

    Directory of Open Access Journals (Sweden)

    Melike Gül

    2015-09-01

    Full Text Available Creating customer-based brand equity is one of the most effective tools for achieving competitive advantage in term of thermal tourism businesses that operating under fierce competition in the last years. The aim of the study is to measure customer-based brand equity perception of customers who accommodate in thermal tourism facilities and identify relationship between brand equity dimensions and size with the other dimensions of each. The survey was conducted on 216 domestic and 184 foreign tourists staying at Balcova Thermal Resort which is located in the Balcova Thermal Destination. Exploratory Factor Analysis and Path Analysis was applied the data. Findings show that there are significant and positive relationship between the size of each customer-based brand equity dimensions. In additions, in order to create ustomer-based brand equity for thermal resorts, some recommendations developed on micro, macro and academic level

  18. Is there a role for {sup 11}C-choline PET/CT in the early detection of metastatic disease in surgically treated prostate cancer patients with a mild PSA increase <1.5 ng/ml?

    Energy Technology Data Exchange (ETDEWEB)

    Castellucci, Paolo [University of Bologna, Service of Nuclear Medicine, Department of Haematology-Oncology and Laboratory Medicine, Azienda Ospedaliero-Universitaria di Bologna, Policlinico Sant' Orsola-Malpighi, Bologna (Italy); Azienda Ospedaliero-Unversitaria di Bologna Policlinico Sant' Orsola-Malpighi, UO di Medicina Nucleare, PAD. 30, Bologna (Italy); Fuccio, Chiara; Santi, Ivan; Nanni, Cristina; Allegri, Vincenzo; Montini, Gian Carlo; Ambrosini, Valentina; Boschi, Stefano; Fanti, Stefano [University of Bologna, Service of Nuclear Medicine, Department of Haematology-Oncology and Laboratory Medicine, Azienda Ospedaliero-Universitaria di Bologna, Policlinico Sant' Orsola-Malpighi, Bologna (Italy); Rubello, Domenico; Marzola, Maria Cristina [Sanata Maria della Misericordia Hospital, Department of Nuclear Medicine, Medical Physics, Radiology, Service of Nuclear Medicine, PET/CT Centre, Rovigo (Italy); Schiavina, Riccardo; Martorana, Giuseppe [University of Bologna, Service of Urology, Department of Specialist Surgery and Anaesthesiology, Azienda Ospedaliero-Universitaria di Bologna Policlinico Sant' Orsola-Malpighi, Bologna (Italy)

    2011-01-15

    -guided biopsy in 7 patients with local recurrence, (b) surgery and lymphadenectomy in 3 patients, (c) other targeted imaging procedures (MR or bone scan) in 5 patients and (d) clinical FU lasting a minimum of 12 months and including also a contrast-enhanced CT (CECT), an MR, a bone scan and a repeated {sup 11}C-choline PET/CT in 14 patients. Age, time to biochemical relapse (TTR), initial T staging, Gleason score and trigger PSA were not statistically significant in predicting a positive {sup 11}C-choline PET/CT scan both at univariate and multivariate analysis. Instead, PSA kinetics (PSAdt and PSAvel), N status and anti-androgenic therapy at the time of PET scan were statistically significant predictive factors at univariate analysis. Of note, only PSAdt and initial N status were found to be significant and independent predictive factors at multivariate analysis. The mean PSAdt in PET-positive patients was 4.34 months (SD 2.82) while in PET-negative patients it was 13.30 months (SD 9.75) (p = 0.0001). The optimal threshold for PSAdt established by receiver-operating characteristic (ROC) analysis was 7.25 months (AUC 0.85; 95% confidence interval 0.77-0.91) providing 93% sensitivity, 74% specificity, 60% positive predictive value and 96% negative predictive value. In our study, {sup 11}C-choline PET/CT was able to detect recurrent disease in 28% of the patients with mild biochemical relapse characterized by very low trigger PSA levels (PSA <1.5 ng/ml). Very interestingly {sup 11}C-choline PET/CT detected distant unexpected metastases in 21% of the patients. At multivariate statistical analysis only PSAdt and node status were shown to be significant and independent predictive factors for positive {sup 11}C-choline PET/CT. Therefore, {sup 11}C-choline could be suggested to be performed early during initial biochemical relapse in patients presenting with fast PSA kinetics. The early detection of the site of recurrence could lead to a prompt instauration of the most appropriate

  19. Postnatal development characteristics of NG2 cells in spinal cords of rats%大鼠脊髓硫酸软骨素蛋白多糖4细胞的生后发育特征

    Institute of Scientific and Technical Information of China (English)

    伍修宇; 唐文强; 孙善全; 漆伟; 张波; 钟小燕; 汪克建

    2012-01-01

    Objective To observe the postnatal development characteristics of NG2 cells in spinal cord of rats. Methods Immunohistochemistry (IHC) was applied to examine the morphologic characteristics of NG2 cells. Western blot was used to observe the expression of NG2 in spinal cord during the different postnatal developmental stages. Results NG2 cells persistently existed during different postnatal developmental stages in spinal cord. There were a great number of NG2 cells with few processes and round or oval cell bodies at Pld. At P7d, a few NG2 cells were detected with short processes in spinal cord. But at P21d,few NG2 cells were found in spinal cord. At P60 d, NG2 cells with irregular cell bodies and 1-3 short processes were found. Compared with the cerebral cortex in the corresponding stages, NG2 cells in spinal cord showed bigger soma, shorter and thin processes. Western blot showed that the expression of NG2 at Pl ,7 ,21 and 60 d was (0. 75 ±0. 11) , (0. 40 ±0. 05) , (0.16 ±0.06) and (0.37 ±0. 06) respectively. NG2 expression was highest at Pld,declined at P7d and reduced significantly to the lowest at P21d,and then increased at P60d. Conclusions The NG2 cells persistently exist in spinal cord and are different in cell number and morphology at the different postnatal development stages. From P7d to P21d may be the major myelination period. NG2 cells in spinal cord may be at an earlier stage in oligodendrocyte lineage.%目的 研究大鼠脊髓中硫酸软骨素蛋白多糖4(NG2)细胞生后发育特征.方法 采用免疫组织化学及Western blot,观察大鼠生后不同发育阶段的脊髓中NG2细胞形态变化.结果 大鼠生后各发育阶段的脊髓内均有NG2阳性细胞表达;NG2细胞的胞体逐渐增大,其形态由圆形或卵圆形变为不规则形,突起数目和长度随之增加,与同时期大脑皮质的NG2细胞形态比较,脊髓内的NG2细胞胞体要大,突起短而少.P1d脊髓内NG2表达值最高,为0.75±0.11,P7d

  20. Preparation of Polymer Drug-loading Micelles of Saponin Rb1 Active Metabolite NG701 and in Vitro Cytotoxicity Evaluation%皂苷Rbl活性代谢产物NG701聚合物载药胶束的制备及其体外细胞毒性评价

    Institute of Scientific and Technical Information of China (English)

    朱宇轩; 贾乙; 张建祥; 李晓辉

    2011-01-01

    OBJECTIVE: To prepare polymer drug-loading micelles of Rb1 active metabolite (NG701), and to evaluate cytotoxicity of it in vitro.METHODS: Amphiphilic copolymer PEG-PBLA was synthesized and then characterized by IR spectrum and HNMR.Dialysis process was used to prepare NG701/PEG-PBLA polymer drug-loading micelles.The morphology and particle size of micelles were observed.The content of NG701 was determined by HPLC, and the drug-loading rate and encapsulation coefficency were calculated.Hela cells were employed as the model to evaluate the cytotoxicity of NG701-loading PEG-PBLA micelles by MTT assay.It was compared with that of NG701 raw material.RESULTS: IR spectrum and HNMR confirmed the synthesis of PEG-PBLA.Prepared micelles were in round shape with size smaller than 200 nm.The drug-loading rate reached 32.7% at the most and encapsulation coefficency was 93.2% at the most.Compared with active concentration of NG701 raw material 100 μg·mL-1, the active concentration of polymer was 50 μg·mL-1 (P<0.05).CONCLUSION: Polymer drug-loading micelles with PEG-PBLA as carrier can improve cytotoxicity of NG 701 effectively.%目的:制备二醇类皂苷Rb1活性代谢产物(NG701)的聚合物载药胶束,并对其进行体外细胞毒性评价.方法:合成两亲性嵌段共聚物即聚乙二醇.聚天冬氨酸苄酯(PEG-PBLA)并借助红外光谱和核磁共振氢谱进行表征;采用透析法制备NG701/PEG.PBLA聚合物载药胶束,观察其形态和粒径分布,建立高效液相色谱法测定其含量并计算载药量和包封率.以Hela细胞为模型,采用MTT法评价聚合物载药胶束的细胞毒性作用,并与NG701原料药比较.结果:红外光谱和核磁共振氢谱证实形成了PEG-PBLA,所制备的聚合物载药胶束呈球形,粒径小于200 nm,载药量最高达32.7%,包封率最高为93.2%.与原料药发挥细胞毒性作用的有效浓度100 μg·mL比较,聚合物载药胶束为50lag·mL(P<0.05).结论:以PEG-PBLA为载体制备的

  1. Thermodynamics of Modifying Effect of Rare Earth Oxide on Inclusions in Hardfaci ng Metal of Medium-High Carbon Steel

    Institute of Scientific and Technical Information of China (English)

    杨庆祥; 赵研辉; 李艳丽; 姚枚

    2002-01-01

    The modifying effect of rare earth (RE) oxide on inclusions in hardfacing metals of medium-high carbon steel was investigated by means of thermodynamics. The t hermodynamic analysis for inclusion formation shows that RE oxide can be redu ced to RE element by carbon, then the RE element can react with oxygen and sulfu r to form the RE oxide, RE sulfide and RE oxide-sulfide in hardfacing molten po ol. The deoxidization and the desulphurization can be carried out and the liquid metal can be purified. In addition, RE oxide can also react with sulfur to form RE oxide-sulfide directly. Therefore, the harmful effect of sulfur can be decr eased.

  2. A GOOD ANTHROPOMETRIC INDEX FOR PREDICTI NG THE RISK OF TYPE 2 DIABETES: WAIST TO HIP RATIO

    Directory of Open Access Journals (Sweden)

    Ramadevi

    2015-07-01

    Full Text Available An increasing trend of type2 Diabetes mellitus is seen with age . Visceral or central obesity is a very common risk factor for developing type 2 diabetes Mellitus. Hence any index which reliably measures obesity can be us ed as a tool to predict the risk of Diabetes. We have attempted to correlate waist to hip ratio (WHR as an anthropometric index to predict the risk of diabetes. When WHR of men is more than 0.9 and women is more than 0.8 the risk increases. MATERIALS & ME THODS: 300 diabetic patients ( 150 males , 150 females and 300 controls (150 males , 150 females were selected and their WHR were measured. The study was done in Gandhi medical college over a period of 2 years. Individuals were grouped according to gender a nd HWR. Data analysis was done by excel sheet and SPSS software. RESULTS: There was significant association between WHR and DM in both males and females. DISCUSSION: Obesity especially visceral leads to insulin resistance and finally DM. This is because adipocytes produce biologic products which modulate insulin action. CONCLUSIONS: Our study proves that HWR screening is a simple and cost effective anthropometric pred ictor of risk for diabetes. This can be used for mass screening as it is quick and needs less expertise.

  3. No differences in metabolic outcomes between nadir GH 0.4 and 1.0 ng/mL during OGTT in surgically cured acromegalic patients (observational study).

    Science.gov (United States)

    Ku, Cheol Ryong; Choe, Eun Yeong; Hong, Jae Won; Kim, Eui Hyun; Park, Se Hee; Kim, Sun Ho; Lee, Eun Jig

    2016-06-01

    Metabolic impairment is the common cause for mortality in acromegalic patients. In this study, long-term improvements of metabolic parameters were evaluated according to 2 different remission criteria.This was an observational cohort study before and up to 1 year after transsphenoidal adenomectomy (TSA). Participants were 187 patients with acromegaly. At 6 months after TSA, remitted patients with age- and sex-matched normalized IGF-1 were divided into 2 groups: remission 1 (R1), nadir growth hormone (GH) below 0.4 ng/mL; and remission 2 (R2), nadir GH between 0.4 and 1.0 ng/mL in oral glucose tolerance test (OGTT). Metabolic parameters during serial OGTTs were evaluated for 12 months. Remission was achieved in 157 (R1-136; R2-21) patients. Immediate postoperative metabolic parameters including body weight, body mass index, glucose, insulin, and free fatty acid in OGTT were all significantly improved in R1 and R2. HOMA-%β and HOMA-IR scores also improved in both R1 and R2. These improvements persisted for duration (12 months) of this study. However, no difference was present in metabolic parameters between R1 and R2. Although the patients with preoperative adrenal insufficiency presented significantly increased HOMA scores before TSA, there was no difference between classifications of deficient pituitary axes and changes of metabolic parameters after TSA. Remitted patients exhibited rapid restoration of metabolic parameters immediate postoperative period. Long-term improvements in metabolic parameters were not different between the 2 different nadir GH cut-offs, 0.4 and 1.0 ng/mL. PMID:27310957

  4. PSA levels of 4.0 - 10 ng/ml and negative digital rectal examination: antibiotic therapy versus immediate prostate biopsy

    Directory of Open Access Journals (Sweden)

    Avraham Shtricker

    2009-10-01

    Full Text Available Purpose: The management of mildly elevated (4.0-10.0 ng/ml prostate specific antigen (PSA is uncertain. Immediate prostate biopsy, antibiotic treatment, or short term monitoring PSA level for 1-3 months is still in controversy. Material and Methods: We conducted a retrospective chart review of patients in a large community practice (2003 - 2007 who had PSA levels between 4.0-10 ng/mL without any further evidence of infection. Data was gathered regarding patient's age, whether standard antibiotic therapy (10-14 days of ofloxacin or ciprofloxacin had been administered before the second PSA measurement, results of a second PSA test performed at 1- to 2-month intervals, whether a prostate biopsy was performed and its result. Results: One-hundred and thirty-five men met the study inclusion criteria with 65 (48.1% having received antibiotics (group 1; the PSA levels decreased in 39 (60% of which, sixteen underwent a biopsy which demonstrated prostate cancer in 4 (25%. Twenty-six (40% patients of group 1 exhibited no decrease in PSA levels; seventeen of them underwent a biopsy that demonstrated cancer in 2 (12%. The other 70 (51.9% patients were not treated with antibiotics (group 2; the PSA levels decreased in 42 (60% of which, thirteen underwent a biopsy which demonstrated prostate cancer in 4 (31%. In the other 28 (40% patients of group 2 there was no demonstrated decrease in PSA, nineteen of these subjects underwent a biopsy that demonstrated cancer in 8 (42%. Conclusions: There appears to be no advantage for administration of antibacterial therapy with initial PSA levels between 4-10 ng/mL without overt evidence of inflammation.

  5. Development of the Next-Generation Caustic-Side Solvent Extraction (NG-CSSX) Process for Cesium Removal from High-Level Tank Waste

    International Nuclear Information System (INIS)

    This paper describes the chemical performance of the Next-Generation Caustic-Side Solvent Extraction (NG-CSSX) process in its current state of development for removal of cesium from the alkaline high-level tank wastes at the Savannah River Site (SRS) in the US Department of Energy (USDOE) complex. Overall, motivation for seeking a major enhancement in performance for the currently deployed CSSX process stems from needs for accelerating the cleanup schedule and reducing the cost of salt-waste disposition. The primary target of the NG-CSSX development campaign in the past year has been to formulate a solvent system and to design a corresponding flowsheet that boosts the performance of the SRS Modular CSSX Unit (MCU) from a current minimum decontamination factor of 12 to 40,000. The chemical approach entails use of a more soluble calixarene-crown ether, called MaxCalix, allowing the attainment of much higher cesium distribution ratios (DCs) on extraction. Concurrently decreasing the Cs-7SB modifier concentration is anticipated to promote better hydraulics. A new stripping chemistry has been devised using a vitrification-friendly aqueous boric acid strip solution and a guanidine suppressor in the solvent, resulting in sharply decreased DCs on stripping. Results are reported herein on solvent phase behavior and batch Cs distribution for waste simulants and real waste together with a preliminary flowsheet applicable for implementation in the MCU. The new solvent will enable MCU to process a much wider range of salt feeds and thereby extend its service lifetime beyond its design life of three years. Other potential benefits of NG-CSSX include increased throughput of the SRS Salt Waste Processing Facility (SWPF), currently under construction, and an alternative modular near-tank application at Hanford.

  6. Value of contrast-enhanced sonographic micro flow imaging for prostate cancer detection with t-PSA level of 4-10 ng/mL

    Energy Technology Data Exchange (ETDEWEB)

    Guo, Yi-Fen, E-mail: guoyifen@gmail.com [Department of Ultrasound, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, 1630 Dongfang Rd., Shanghai 200127 (China); Li, Feng-Hua, E-mail: li_fenghua3211@163.com [Department of Ultrasound, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, 1630 Dongfang Rd., Shanghai 200127 (China); Xie, Shao-Wei, E-mail: shaoweixie@yahoo.cn [Department of Ultrasound, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, 1630 Dongfang Rd., Shanghai 200127 (China); Xia, Jian-Guo, E-mail: doctorxiajg@yahoo.com.cn [Department of Ultrasound, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, 1630 Dongfang Rd., Shanghai 200127 (China); Fang, Hua, E-mail: huafang12@126.com [Department of Ultrasound, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, 1630 Dongfang Rd., Shanghai 200127 (China); Li, Hong-Li, E-mail: lihliwl@163.com [Department of Ultrasound, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, 1630 Dongfang Rd., Shanghai 200127 (China)

    2012-11-15

    Objectives: To compare the efficiency of contrast-enhanced ultrasonographic micro flow imaging (MFI) with conventional transrectal ultrasound (TRUS) in detecting prostate cancer with serum total prostate-specific antigen (t-PSA) of 4.0-10.0 ng/mL. To evaluate the value of contrast-enhanced ultrasonographic MFI in detecting prostate cancer with t-PSA in diagnostic gray zone. Methods: 47 patients with t-PSA 4.0-10.0 ng/mL underwent gray scale, power Doppler TRUS and MFI examinations before ultrasound guided biopsies. Biopsies were performed at twelve sites in the base, the mid-gland and the apex of the prostate in each patient, when there was no abnormal ultrasound finding. When an abnormality was present at MFI, the biopsy specimen from the corresponding site was directed toward the abnormal finding. With histological results of prostate biopsy as reference standards, we assessed the cancer detection of these three methods. Results: 564 specimens were collected in this study, in which 101 were prostate cancer confirmed histologically. 152 of 564 specimens were demonstrated abnormal on MFI images, in which 71 were malignant and 81 were benign confirmed histologically. The sensitivity, specificity, accuracy, positive predictive value (PPV) and negative predictive value (NPV) for MFI in detecting prostate caner were 70.3%, 82.5%, 80.3%, 46.7% and 92.7%, respectively. The sensitivity and NPV for MFI were significantly better than gray scale (38.6%, 86.9%) and power Doppler (32.7%, 86.0%) (P < 0.001) TRUS. Conclusions: Contrast-enhanced ultrasonographic MFI could significantly improve the detection rate of prostate cancer with t-PSA in diagnostic gray zone (4-10 ng/mL) than conventional ultrasound.

  7. Dissociation of castor oil-induced diarrhoea and intestinal mucosal injury in rat: effect of NG-nitro-L-arginine methyl ester.

    OpenAIRE

    Capasso, F; Mascolo, N; Izzo, A A; Gaginella, T S

    1994-01-01

    1. Castor oil (2 ml orally) produced diarrhoea in rats 1-7 h after challenge, which was associated with gross damage to the duodenal and jejunal mucosa. 2. The injury was accompanied by release of acid phosphatase into the gut lumen, indicating cellular injury. 3. Intraperitoneal injection of the nitric oxide (NO) synthase inhibitor NG-nitro-L-arginine methyl ester (L-NAME, 2.5-50 mg kg-1 twice), prevented the diarrhoea. The dose of L-NAME (50 mg kg-1) completely blocked the diarrhoea but inc...

  8. Depression of high-threshold calcium currents by activation of human D2 (short) dopamine receptors expressed in differentiated NG108-15 cells.

    OpenAIRE

    Seabrook, G. R.; McAllister, G.; Knowles, M. R.; Myers, J.; Sinclair, H; Patel, S.; Freedman, S B; Kemp, J.A.

    1994-01-01

    1. This study examined the regulation of calcium currents in differentiated NG108-15 cells that had been stably transfected with cDNA encoding the short isoform of the human D2 dopamine receptor. Whole cell calcium currents were recorded by nystatin-perforated patch clamp recording. 2. Transient low-threshold calcium currents elicited by depolarizations from -100 mV to -20 mV were reversibly depressed by NiCl2 (84 +/- 8% at 30 microM; n = 3) and by omega-agatoxin IVA (15 +/- 5%; 100 nM, n = 7...

  9. Crianças bilíngües Alemão-Português: erros na escrita e características do ambiente familiar Bilingual German-Portuguese children: handwriting mistakes and characteristics of the familiar environment

    Directory of Open Access Journals (Sweden)

    Simone Raquel Sbrissa Moura

    2008-01-01

    Full Text Available OBJETIVO: Relacionar erros na escrita em português, características do ambiente familiar, idade e sexo de crianças bilíngües Alemão-Português. MÉTODOS: Vinte crianças bilíngües alemão/português realizaram ditado balanceado, e seus pais responderam a um questionário. Os tipos de erros na escrita em Português foram classificados e quantificados, e posteriormente relacionados às respostas dos pais no questionário aplicado. RESULTADOS: Houve menos erros relacionados à troca, substituição aleatória, inversão, transposição, omissão e adição de grafemas, e mais erros relacionados às irregularidades da língua portuguesa. A maioria dos pais possuía material escrito em casa; quando mais de uma pessoa lia em casa e quanto maior a escolaridade paterna, maior o número de crianças com menos erros na escrita; quanto menor a escolaridade materna, maior o número de estudantes com mais erros na escrita. Quanto mais novas as crianças, mais erros de todos os tipos. Os meninos apresentaram mais erros na escrita do que as meninas. CONCLUSÕES: Mesmo sendo os participantes deste estudo todos bilíngües, a maioria dos achados foi compatível com os encontrados em outras pesquisas com crianças monolíngües do Português Brasileiro.PURPOSE: To relate mistakes in Portuguese writing, characteristics of the familiar environment, age and gender of bilingual German-Portuguese children. METHODS: Twenty bilingual children were submitted to balanced dictation, and their parents answered a questionnaire. The analysis classified and quantified the types of errors in Portuguese writing, which was later related to their parents' answers on the questionnaire. RESULTS: There were fewer errors related to change, random substitution, inversion, transposition, omission and adding of graphemes, and more errors associated with Portuguese irregularities. Most parents had written material at home; when more than one person used to read at home and the

  10. AN INVESTİGATİON OF THE EFFECTİVENESS OF MATHEMATİCS TEACHİNG İN A LEARNİNG ENVİRONMENT DESİGNED ACCORDİNG TO THE THEORY OF MULTİPLE INTELLİGENCES

    Directory of Open Access Journals (Sweden)

    Ramazan GÜRBÜZ

    2013-06-01

    Full Text Available The aim of this study is to evaluate qualitatively the themes emerging from the application of activities designed according to The Theory of Multiple Intelligences. The activities were selected from the learning domains of 7th grade mathematics curriculum. This case study was conducted with two mathematics teachers from different schools and their 54 students and it lasted total 51 hours. Interview and observation methods were used in order to collect data as well as student journals. After a content analysis of the interviews, codes and themes were derived with regard to the purpose of the study. Observations and student journals were presented by directly quoting the parts related with the themes derived from the interviews. As a result of this study, the activities carried out in environments designed according to The Theory of Multiple Intelligences were found to be effective in the context of the theme of "facilitating understanding and providing permanent learning". Since this kind of practices have the role of catalyzing in the the displacement process of learning environments based on alternative learning theories by traditional learning environments, the frequent implementation of similar practices in different subject fields and at different levels should be implemented for the widespread use of student centered approaches.

  11. Assay-specific artificial neural networks for five different PSA assays and populations with PSA 2-10 ng/ml in 4,480 men.

    Science.gov (United States)

    Stephan, Carsten; Xu, Chuanliang; Cammann, Henning; Graefen, Markus; Haese, Alexander; Huland, Hartwig; Semjonow, Axel; Diamandis, Eleftherios P; Remzi, Mesut; Djavan, Bob; Wildhagen, Mark F; Blijenberg, Bert G; Finne, Patrik; Stenman, Ulf-Hakan; Jung, Klaus; Meyer, Hellmuth-Alexander

    2007-03-01

    Use of percent free PSA (%fPSA) and artificial neural networks (ANNs) can eliminate unnecessary prostate biopsies. In a total of 4,480 patients from five centers with PSA concentrations in the range of 2-10 ng/ml an IMMULITE PSA-based ANN (iANN) was compared with other PSA assay-adapted ANNs (nANNs) to investigate the impact of different PSA assays. ANN data were generated with PSA, fPSA (assays from Abbott, Beckman, DPC, Roche or Wallac), age, prostate volume, and DRE status. In 15 different ROC analyses, the area under the curve (AUC) in the PSA ranges 2-4, 2-10, and 4-10 ng/ml for the nANN was always significantly larger than the AUC for %fPSA or PSA. The nANN and logistic regression models mostly also performed better than the iANN. Therefore, for each patient population, PSA assay-specific ANNs should be used to optimize the ANN outcome in order to reduce the number of unnecessary biopsies. PMID:17333205

  12. Remaining oil distribution in Ng33 bottom water reservoir of Lin 2-6 fault-block in Huimin depression and potential tapping in horizontal well

    Institute of Scientific and Technical Information of China (English)

    HAN Zuo-zhen; YANG Ren-chao; FAN Ai-ping; CHEN Qing-chun; SHAO Yun-tang

    2009-01-01

    Oil reservoirs with secondary bottom water in Ng33 members (in Guantao formation, Paleogene system) of Lin2-6 fault block in Huimin depression (Bohai Bay Basin) have entered the late stage of ultra-high water-containing-exploitation. Oil exploita-tion from vertical wells is becoming more and more inefficient. The reservoir type, with water displacing oil and the remaining oil distribution are specifically studied in order to improve the efficiency of the recovery ratio. An integrated scheme for adjusting horizontal wells has been designed and the key technique of the scheme optimized. The study shows that: 1) the positive rhythm of fluvial depositional features is the internal cause of the flooding of oil reservoirs while water injection, injection-production patterns and accumulative petroleum production are the external causes; 2) oil-water driving patterns have transferred from edge water ad-vancing to bottom-water-coning; distribution of the remaining oil mainly concentrates in the upper rhythm and top of the middle rhythm in Ng33 members; 3) a great deal of remaining oil is enriched in high positions of faults, in axes of tiny structures, in stagna-tion areas among water-injection wells and oil-wells and in tectonic saddle areas with sparse wells. Compared with vertical wells, horizontal wells have advantages such as high recovery, high off-take potential, high critical output, large controlling areas and long time of bottom-water breakthrough.

  13. Effects of androgen on phosphacan and NG2 proteoglycan expression and neuronal axial regeneration in traumatic brain injury rat model%雄激素对创伤性脑损伤模型大鼠磷酸蛋白聚糖和NG2蛋白聚糖表达与神经元轴突再生影响的研究

    Institute of Scientific and Technical Information of China (English)

    陈玉红; 仝海波; 段虎斌

    2014-01-01

    目的:了解雄激素对创伤性脑损伤(TBI)大鼠脑组织磷酸蛋白聚糖(PC)及NG2蛋白聚糖表达影响及其与TBI后神经修复的关系,探讨其神经保护及再生的作用机制。方法制作大鼠TBI模型,随机分为正常组、TBI模型组、雄激素干预组(于模型制成后即刻注射丙酸睾丸酮注射液25 mg/kg)。造模后1、3、5、7 d取脑组织制作石蜡切片和电镜切片,用免疫组织化学法观察PC和NG2在损伤区表达的动态变化,应用透射电镜对比观察损伤区及其周围的神经元超微结构变化、细胞损伤、凋亡、神经元轴突再生及胶质细胞增生情况。结果①TBI模型组造模后1 d可见脑细胞肿胀、凋亡,3、7 d细胞水肿,可见凋亡细胞、胶质细胞于损伤外围增生;雄激素干预组1、3、5和7 d时细胞水肿,可见凋亡细胞、损伤外围胶质细胞增生情况明显降低,TBI模型组细胞表面无突起或仅有较小突起;雄激素干预组的神经元轴突较TBI模型组增长明显。②造模后脑组织PC和NG2的表达:正常组脑组织均存在少量PC和NG2的表达;TBI模型组在造模后1 d表达开始呈阳性反应,造模后3 d明显增多,7 d达高峰,但仍高于正常组;雄激素干预组,PC和NG2的表达时间程度及分布与TBI模型组相似,雄激素干预组造模后1、3、5、7 d后损伤区及周围表达水平明显低于TBI模型组,差异均有统计学意义(P<0.01)。结论雄激素可通过调节胶质细胞形态结构和功能抑制PC和NG2表达促进神经元轴突再生,从而发挥神经保护。%Objective To study the effects of androgen on phosphacan (PC) and NG2 proteoglycan (NG2) ex-pression and neuronal regeneration in traumatic brain injury (TBI) rat models, thus unraveling the potential mecha-nisms underlying the protective effects of androgen against TBI. Methods Following construction of TBI models, the rats were randomly

  14. THROUGH THE INTERVIEW TEXTS THE ANALYSIS OF THE 8TH GRADE STUDENTS' SKİLLS OF ASKİNG QUESTIONS ACCORDING TO THE REVISED BLOOM'S TAXONOMY

    OpenAIRE

    Yilmaz, Engin; KERAY, Betül

    2013-01-01

    This research has been made in order to determine how effective the education of asking questions is on students and in which stage the students achieve the acqusition “Forming questions about the text” which is stated in 8th grade students' Turkish Teaching Program. For this research, a school located in city centre in Aksaray was chosen, and one of the two classes- both of which have been taught by the same teacher- is selected conrol group and the the other is selected experiment group. Th...

  15. Experimental and theoretical analysis of effects of N2, O2 and Ar in excess air on combustion and NOx emissions of a turbocharged NG engine

    International Nuclear Information System (INIS)

    Highlights: • We defined 7 effects of excess air and quantitative indicators on NOx emissions. • Contributions of N2, O2 and Ar on the thermal effect are separately 85%, 14% and 1%. • Contributions of N2 and Ar on the dilution effect are 98.8% and 1.2%, respectively. • The high level of N2 in air is the main reason why NOx emissions decrease with λ. - Abstract: This paper presents the effects of N2, O2 and Ar in excess air on combustion and NOx emissions of a natural gas spark-ignition engine. Based on Arrhenius Law and Zeldovich Mechanism, seven different kinds of effects were defined as well as their quantitative indicators. Excess air and N2 were separately added into the intake charge with fuel remaining unchanged in a 6-cylinder turbocharged natural gas engine. The results show that the essential reason why NOx emissions vary with increasing excess air ratio (λ) is that the oxidation effect of O2 varies with the in-cylinder temperature, which is controlled by the thermal effect of excess air. The contributions of N2, O2 and Ar on the thermal effect are 82.6–86.1%, 13.3–16.7% and 0.6–0.7%, respectively. The chemical effect of O2 is fully offset by the dilution effect of N2 and Ar. The contributions of N2 and Ar on the dilution effect are 98.31% and 1.69%, respectively. The increase of NOx emissions caused by O2 is found to be increased first and then decreased with increasing λ. From the view of the composition of air, the high level of N2 is the main reason why NOx emissions decrease significantly with increasing λ

  16. Andrew Ng 谈 Deep Learning

    Institute of Scientific and Technical Information of China (English)

    卢鸫翔

    2014-01-01

    Andrew Ng是Deep Learning领域最富盛名的科学家之一,他也是斯坦福人工智能实验室主任,在线教育平台Coursera的创始人之一。2014年5月,他加入百度,负责“百度大脑”计划,并担任百度首席科学家。目前,他接受了《程序员》的专访。

  17. {delta}-Opioid receptor-stimulated Akt signaling in neuroblastoma x glioma (NG108-15) hybrid cells involves receptor tyrosine kinase-mediated PI3K activation

    Energy Technology Data Exchange (ETDEWEB)

    Heiss, Anika; Ammer, Hermann [Institute of Pharmacology, Toxicology and Pharmacy Ludwig-Maximilians-University of Munich Koeniginstrasse 16 80539 Muenchen Federal Republic of Germany (Germany); Eisinger, Daniela A., E-mail: eisinger@pharmtox.vetmed.uni-muenchen.de [Institute of Pharmacology, Toxicology and Pharmacy Ludwig-Maximilians-University of Munich Koeniginstrasse 16 80539 Muenchen Federal Republic of Germany (Germany)

    2009-07-15

    {delta}-Opioid receptor (DOR) agonists possess cytoprotective properties, an effect associated with activation of the 'pro-survival' kinase Akt. Here we delineate the signal transduction pathway by which opioids induce Akt activation in neuroblastoma x glioma (NG108-15) hybrid cells. Exposure of the cells to both [D-Pen{sup 2,5}]enkephalin and etorphine resulted in a time- and dose-dependent increase in Akt activity, as measured by means of an activation-specific antibody recognizing phosphoserine-473. DOR-mediated Akt signaling is blocked by the opioid antagonist naloxone and involves inhibitory G{sub i/o} proteins, because pre-treatment with pertussis toxin, but not over-expression of the G{sub q/11} scavengers EBP50 and GRK2-K220R, prevented this effect. Further studies with Wortmannin and LY294002 revealed that phophoinositol-3-kinase (PI3K) plays a central role in opioid-induced Akt activation. Opioids stimulate Akt activity through transactivation of receptor tyrosine kinases (RTK), because pre-treatment of the cells with inhibitors for neurotrophin receptor tyrosine kinases (AG879) and the insulin-like growth factor receptor IGF-1 (AG1024), but not over-expression of the G{beta}{gamma} scavenger phosducin, abolished this effect. Activated Akt translocates to the nuclear membrane, where it promotes GSK3 phosphorylation and prevents caspase-3 cleavage, two key events mediating inhibition of cell apoptosis and enhancement of cell survival. Taken together, these results demonstrate that in NG108-15 hybrid cells DOR agonists possess cytoprotective properties mediated by activation of the RTK/PI3K/Akt signaling pathway.

  18. Mechanised flow system for on-line microwave digestion of food samples with off-line catalytic spectrophotometric determination of cobalt at ng l-1 levels.

    Science.gov (United States)

    Pereira-Filho, E R; Arruda, M A

    1999-12-01

    A mechanised system for on-line slurry food sample digestion was developed and an off-line cobalt determination was performed. The stabilised slurry sample was introduced into an air carrier stream until reaching the digestion coils located inside a household microwave oven. Software written in Visual Basic 3.0 was developed to permit the transport of the slurry samples and the programming of the microwave oven and also the control of the mineralization valve. The proposed system was optimized for determination of cobalt in certified samples such as mussels, bovine liver and fish and also uncertified fish samples. The digestion parameters were established as 3 mol l-1 HNO3 for mussels, 3 mol l-1 HNO3 plus 0.16% v/v H2O2 for bovine liver and 12 mol l-1 HNO3 for fish employing maximum power for 5 min of microwave actuation. In the subsequent spectrophotometric method for the catalytic determination of cobalt, the Tiron and hydrogen peroxide concentrations were 1.8 x 10(-3) and 3.0 x 10(-4) mol l-1, respectively, and the sample residence time was 300 s as determined by an optimisation process. The proposed method features a linear range from 10 to 200 ng l-1 Co (r > 0.996) with detection and quantification limits of 1.7 and 5.5 ng l-1 Co, respectively. The precision, expressed as RSD, was 2.4% (n = 12) for repeatability and 5.2% (n = 10) for reproducibility and the accuracy of the proposed method was assessed by using certified samples and an alternative technique (ETAAS). PMID:10746313

  19. Evaluation of the Bio-Rad Dx CT/NG/MG® assay for simultaneous detection of Chlamydia trachomatis, Neisseria gonorrhoeae and Mycoplasma genitalium in urine.

    Science.gov (United States)

    Ursi, D; Crucitti, T; Smet, H; Ieven, M

    2016-07-01

    We evaluated the performance of the Bio-Rad real-time Dx CT/NG/MG® assay for detection of C. trachomatis, N. gonorrhoeae and M. genitalium on a collection of 441 urine samples from sexually transmitted infections, or travellers consultations and from anonymous sperm donors that were previously analysed with the Abbott RealTime CT/NG assay. Samples positive for C. trachomatis or N. gonorrhoeae with the Abbott assay had all previously been confirmed with an in-house real-time PCR assay. Samples positive for M. genitalium with the Bio-Rad assay were subsequently analysed by an in-house real-time PCR. On a total of 441 urines, 104 samples were positive for C. trachomatis, 12 were positive for N. gonorrhoeae and seven were positive for M. genitalium. After retesting of discrepant results, the test results were completely concordant, resulting in a calculated sensitivity and specificity of the Bio-Rad assay of 98.1 % and 100 % for C. trachomatis and of 91.7 % and 100 % for N. gonorrhoeae. Results for M. genitalium with the Bio-Rad assay were also concordant with the results of an in house PCR. We also evaluated the performance of automated nucleic acid extractions of urine samples with the NucliSENS easyMAG (bioMérieux) compared to the manual DNA extraction prescribed by the insert of the kit. The easyMAG extraction gave lower Ct values, relieved inhibition and had a lower hands-on time. PMID:27116008

  20. Exploração de fatores de risco para câncer de mama em mulheres de etnia Kaingáng, Terra Indígena Faxinal, Paraná, Brasil, 2008 Exploring breast cancer risk factors in Kaingáng women in the Faxinal Indigenous Territory, Paraná State, Brazil, 2008

    Directory of Open Access Journals (Sweden)

    Edimara Patrícia da Silva

    2009-07-01

    Full Text Available O objetivo do presente estudo foi analisar a saúde das mulheres Kaingáng da Terra Indígena Faxinal, Paraná, Brasil, quanto aos fatores de risco para o câncer de mama. O estudo, de caráter descritivo-exploratório, foi realizado com 95,4% da população feminina com vinte anos ou mais. O aleitamento materno por mais de 1 ano foi relatado por 92,3% das mulheres; 41,4% tinham mais de cinco filhos; a idade média da menarca foi 13,4 anos; 61,5% nunca tinham ouvido falar do câncer de mama; o auto-exame da mama era realizado por apenas 2,9% das mulheres; o médico não realizava o exame clínico da mama, segundo 81,7% delas, e 99% nunca fizeram mamografia. Não foi relatado caso de câncer de mama ou extramamário. Destacaram-se a presença dos fatores de proteção para a carcinogênese e a ausência de alguns fatores de risco, como o uso de terapia de reposição hormonal por mais de cinco anos e etilismo, na maioria das mulheres Kaingáng.This study analyzes the health of Kaingáng women in the Faxinal indigenous territory in Paraná State, Brazil, with regard to risk factors for breast cancer. A descriptive and exploratory investigation was undertaken that included 95.4% of females 20 years or over. Data showed that 92.3% of Kaingáng women reported breastfeeding for more than one year; 41.4% had more than 5 children; mean age at menarche was 13.4 years; 61.5% had never heard of breast cancer; breast self-examination was only performed by 2.9%; 81.7% reported no clinical breast examination by a physician; 99% had never had a mammogram. No cases of breast or non-breast cancer were reported. The study highlights protective factors against carcinogenesis and the absence of key risk factors like hormone replacement therapy for more than five years and alcohol consumption in most Kaingáng women.

  1. Ang Paghiraya sa Nasyon: Ang mga Pagdiriwang ng Anibersaryo ng Komonwelt ng Pilipinas (1936-1941

    Directory of Open Access Journals (Sweden)

    Michael Charleston B. Chua

    2007-12-01

    Full Text Available The paper will revisit the forms and themes of commemoration of the anniversary of the Philippine Commonwealth every November 15 from 1936 to 1941. Similar to the present-day State of the Nation Address, the celebrations reflected the mood and sentiments of the government and served as the articulation of how the ruling elite democracy imagined the nation that would be established under the guidance of the United States of America. (1 The plans for national defense, (2 the loyalty of America, (3 the achievements of the Commonwealth government, (4 optimism for the future, (5 Pres. Manuel Quezon, the Great Leader, and (6 the excellence and capabilities of the Filipino, became the common themes of the celebrations reinforced yearly to rally the citizens to imagine the nation with the government in a ritualistic gathering.

  2. Prostate Health Index (Phi and Prostate Cancer Antigen 3 (PCA3 significantly improve prostate cancer detection at initial biopsy in a total PSA range of 2-10 ng/ml.

    Directory of Open Access Journals (Sweden)

    Matteo Ferro

    Full Text Available Many efforts to reduce prostate specific antigen (PSA overdiagnosis and overtreatment have been made. To this aim, Prostate Health Index (Phi and Prostate Cancer Antigen 3 (PCA3 have been proposed as new more specific biomarkers. We evaluated the ability of phi and PCA3 to identify prostate cancer (PCa at initial prostate biopsy in men with total PSA range of 2-10 ng/ml. The performance of phi and PCA3 were evaluated in 300 patients undergoing first prostate biopsy. ROC curve analyses tested the accuracy (AUC of phi and PCA3 in predicting PCa. Decision curve analyses (DCA were used to compare the clinical benefit of the two biomarkers. We found that the AUC value of phi (0.77 was comparable to those of %p2PSA (0.76 and PCA3 (0.73 with no significant differences in pairwise comparison (%p2PSA vs phi p = 0.673, %p2PSA vs. PCA3 p = 0.417 and phi vs. PCA3 p = 0.247. These three biomarkers significantly outperformed fPSA (AUC = 0.60, % fPSA (AUC = 0.62 and p2PSA (AUC = 0.63. At DCA, phi and PCA3 exhibited a very close net benefit profile until the threshold probability of 25%, then phi index showed higher net benefit than PCA3. Multivariable analysis showed that the addition of phi and PCA3 to the base multivariable model (age, PSA, %fPSA, DRE, prostate volume increased predictive accuracy, whereas no model improved single biomarker performance. Finally we showed that subjects with active surveillance (AS compatible cancer had significantly lower phi and PCA3 values (p<0.001 and p = 0.01, respectively. In conclusion, both phi and PCA3 comparably increase the accuracy in predicting the presence of PCa in total PSA range 2-10 ng/ml at initial biopsy, outperforming currently used %fPSA.

  3. Molecular detection and confirmation of Neisseria gonorrhoeae in urogenital and extragenital specimens using the Abbott CT/NG RealTime assay and an in-house assay targeting the porA pseudogene.

    LENUS (Irish Health Repository)

    Walsh, A

    2011-04-01

    Culture for detection of Neisseria gonorrhoeae (NG) is being replaced by molecular assays, but difficulties are observed with false positive and negatives results, especially for extragenital samples. This study evaluates the Abbott CT\\/NG Real-Time assay and a real-time porA pseudogene assay. Samples (n = 600) from a mixed prevalence Irish population include 164 male urines with corresponding urethral swabs, 58 endocervical swabs, 173 male pharyngeal swabs, 205 male rectal swabs, 36 NG clinical isolates and 26 commensal Neisseria species isolates. There was a 100% concordance between the Abbott CT\\/NG Real-Time and the porA assay. The positivity rate was 1.2%, 1.7%, 8.1% and 5.8% for FVU\\/urethral swabs, endocervical, pharyngeal and rectal swabs, respectively. These results were compared to culture and discrepancies were found with nine pharyngeal and three rectal swabs. Seven of the 12 discrepant positive samples were sequenced and were confirmed "true positives". The sensitivity and specificity of the molecular assays was 100%. The sensitivity of the culture-based testing was 100% for urogenital samples but 36% and 75% for pharyngeal and rectal swabs, respectively. The combined Abbott CT\\/NG and porA assays provide a valuable alternative to culture and also generate a significant increase in the diagnosis of pharyngeal and rectal NG infection.

  4. ACE Cycle in Programming Education by Using Visualization [Programlama Eğitiminde Görselleştirme ile ACE Döngüsü

    Directory of Open Access Journals (Sweden)

    İbrahim Çetin

    2014-12-01

    Full Text Available Students experience difficulties in learning computer programming. Researchers have conducted several studies with different perspectives to help students learn programming. The ACE cycle constructed in the context of mathematics education was adapted to programming education and called PACE cycle. The aim of the study was to test effectiveness of the PACE cycle by using both quantitative and qualitative measures. The sample of the study included 62 mechanical engineering students. The students were randomly assigned to control and experimental groups. Experimental group was instructed by using PACE cycle whereas control group was instructed by using ‘traditional instruction’. Both quantitative and qualitative data were gathered before and after the treatment. It was found that there is no significant difference between achievement and attitude scores of experimental and control group students. Nevertheless, it is hard to announce PACE cycle as ineffective depending only on this result. Qualitative data showed that students, in the control group, did not percieve their instruction as traditional one. This might show us that the definition of ‘traditional instruction’ can change depending on the context. It might be the case that students’ perception and experience related to their instruction affected the results of the study. [Öğrenciler bilgisayar programlamayı öğrenirken çeşitli zorluklarla karşılaşmaktadır. Araştırmacılar öğrencilere bu zorlukları aşmada yardımcı olmak için çalışmalar yapmışlardır. Bu çalışmada matematik eğitimi bağlamında geliştirilmiş olan ACE döngüsü isimli öğretim ortamı programlama eğitimine PACE döngüsü olarak uyarlanmıştır. Çalışmanın amacı PACE döngüsünün etkililiğini nicel ve nitel boyutlarıyla araştırmaktır. Çalışmanın örneklemini 62 makine mühendisliği öğrencisi oluşturmaktadır. Öğrenciler deney ve kontrol gruplarına rastgele

  5. Inhibition of Voltage-gated Sodium Channel in NG108-15 by the Venoms from Three Kinds of Spider%三种蜘蛛粗毒对NG108-15细胞电压门控钠通道的抑制作用

    Institute of Scientific and Technical Information of China (English)

    肖玉成; 彭宽; 陈湘定; 梁宋平

    2001-01-01

    Using whole-cell patch-clamp technology, the effects of the venoms from three kinds of spider (Selenocosmia huwena, Selenocosmia hainana and Macrothele guangxia sp) on voltage-activated TTX-sensitive(TTX-S) Na+ current and delay-rectified K+ current of undifferentiated NG108-15 were observed. The results showed that they had no evident effect of outword delay-rectified K+ current,while they could inhibit TTX-S fast Na+ current.The inhibitions were dose-dependent.The EC50 values of them were approximate 3.4 mg/L(Selenocosmia huwena); 1.8 mg/L(Selenocosmia hainana);11.0 mg/L(Macrothele guangxia sp),respectively.%利用小鼠神经细胞瘤×大鼠神经胶质细胞的杂交细胞NG108-15,通过全细胞记录(whole-cell recording)模式的膜片钳技术,检验了虎纹捕鸟蛛(Selenocosmia huwena)、海南捕鸟蛛(Selenocosmia hainana)和广西大疣蛛(Macrothele guangxia sp)的粗毒对NG108-15细胞膜上电压门控TTX敏感型钠电流和延迟整流钾电流的作用.结果表明,三种蜘蛛粗毒对外向延迟整流钾电流没有明显作用,但对TTX敏感型的快钠电流表现出较强的抑制效应.抑制效应呈量效关系.三种粗毒抑制钠电流的EC50相应约为:3.4 mg/L;1.8 mg/L和11.0 mg/L.

  6. Simultaneous recording of the action potential and its whole-cell associated ion current on NG108-15 cells cultured over a MWCNT electrode

    Science.gov (United States)

    Morales-Reyes, I.; Seseña-Rubfiaro, A.; Acosta-García, M. C.; Batina, N.; Godínez-Fernández, R.

    2016-08-01

    It is well known that, in excitable cells, the dynamics of the ion currents (I i) is extremely important to determine both the magnitude and time course of an action potential (A p). To observe these two processes simultaneously, we cultured NG108-15 cells over a multi-walled carbon nanotubes electrode (MWCNTe) surface and arranged a two independent Patch Clamp system configuration (Bi-Patch Clamp). The first system was used in the voltage or current clamp mode, using a glass micropipette as an electrode. The second system was modified to connect the MWCNTe to virtual ground. While the A p was recorded through the micropipette electrode, the MWCNTe was used to measure the underlying whole-cell current. This configuration allowed us to record both the membrane voltage (V m) and the current changes simultaneously. Images acquired by atomic force microscopy (AFM) and scanning electron microscopy (SEM) indicate that cultured cells developed a complex network of neurites, which served to establish the necessary close contact and strong adhesion to the MWCNTe surface. These features were a key factor to obtain the recording of the whole-cell I i with a high signal to noise ratio (SNR). The experimental results were satisfactorily reproduced by a theoretical model developed to simulate the proposed system. Besides the contribution to a better understanding of the fundamental mechanisms involved in cell communication, the developed method could be useful in cell physiology studies, pharmacology and diseases diagnosis.

  7. The transmission of symmetric 40 Gb/s TWDM-based NG-PON2 utilizing delay interferometer (DI) for RSOA bandwidth enhancement

    Science.gov (United States)

    Bindhaiq, Salem; Zulkifli, Nadiatulhuda; Supa'at, AbuSahmah M.

    2016-07-01

    Time and wavelength-division multiplexed passive optical network (TWDM-PON) has been finally selected as the pragmatic solution for the next-generation passive optical network stage 2 (NG-PON2). In this paper, we propose a symmetric 40 Gb/s TWDM-PON system with low cost reflective semiconductor optical amplifier (RSOA) for both downstream and upstream directions. A single bi-pass delay interferometer (DI), deployed in the optical line terminal (OLT), is used to enhance the poor performance of the RSOA with respect to the low bandwidth induced by laser chirp. With the help of the 40 GHz free spectrum range (FSR) DI, we show a successful transmission of the proposed work through simulation study where an aggregate capacity of 40 Gb/s is transported over 40 km transmission distance with 32 splits. The TWDM-PON system at BER of 10-6 has shown a minimum receiver sensitivity of -22.78 dBm and -22.71 dBm for both downstream and upstream, respectively with maximum power penalty of 2 dB for downstream channel and 2.39 dB for upstream channel.

  8. A layered magnetic iron/iron oxide nanoscavenger for the analytical enrichment of ng-L(-1) concentration levels of heavy metals from water.

    Science.gov (United States)

    Karatapanis, Andreas E; Petrakis, Dimitrios E; Stalikas, Constantine D

    2012-05-13

    Magnetically driven separation techniques have received considerable attention in recent decade because of their great potential application. In this study, we investigate the application of an unmodified layered magnetic Fe/Fe(2)O(3) nanoscavenger for the analytical enrichment and determination of sub-parts per billion concentrations of Cd(II), Pb(II), Ni(II), Cr(VI) and As(V) from water samples. The synthesized nanoscavenger was characterized by BET, TGA, XRD and IR and the parameters influencing the extraction and recovery of the preconcentration process were assessed by atomic absorption spectrometry. The possible mechanism of the enrichment of heavy metals on Fe/Fe(2)O(3) was proposed, which involved the dominant adsorption and reduction. The nanoscale size offers large surface area and high reactivity of sorption and reduction reactions. The obtained limits of detection for the metals studied were in the range of 20-125 ng L(-1) and the applicability of the nanomaterial was verified using a real sample matrix. The method is environmentally friendly as only 15 mg of nanoscavenger are used, no organic solvent is required for the extraction and the experiment is performed without the need for filtration or preparation of packed preconcentration columns.

  9. Marketingový plán pro letoun L410 NG společnosti Aircraft Industries, a.s.

    OpenAIRE

    Juráková, Hana

    2014-01-01

    Tato diplomová práce se zabývá sestavením marketingového plánu pro letoun L 410 NG, vyráběný firmou Aircraft Industries a.s. První část práce zahrnuje obecné teoretické poznatky, zaměřující se na proces sestavení plánu pomocí rozboru vnějšího okolí SLEPTE, Porterova modelu pěti konkurenčních sil, analýzy SWOT a marketingového mixu 5P. Ve druhé analytické části je tato základní teorie aplikována na uvedenou firmu. V závěrečné části práce jsou na základě zjištěných fakt a výsledků analýz navrhn...

  10. Protection of panaxnotoginsengsaponins against NG108-15 cells in senile dementia model induced by amyoid beta-peptide 25- 35%三七总皂甙对淀粉样β25~35肽诱导的NG108-15细胞老年性痴呆模型的保护作用

    Institute of Scientific and Technical Information of China (English)

    卢忠朋; 王乃平; 钟振国

    2004-01-01

    背景:阿尔茨海默病(Alzheimer disease,AD)的病理病因虽然还没有完全清楚,但目前认为β-淀粉样肽(amyloid β-peptide,Aβ)是引起AD的胆碱能神经功能紊乱的主要成分.因此,Aβ的产生及其对神经细胞的影响,以及寻找该病的发病机制、治疗方法及其有效的治疗药物成了医药界的研究热点.目的:观察三七总皂甙(PNS)对NG108-15细胞老年性痴呆模型的影响,为寻找该病的发病机制提供佐证,为研究和开发中药的新成分提供理论依据.设计:体外平行对照实验.单位:广西中医学院新药开发中心,广西中医学院生物化学教研室.对象:实验在广西中医学院新药开发中心完成.NG108-15细胞:中国科学院上海细胞研究所提供.PNS:云南玉溪维和制药厂生产.Aβ25~35片段:德国Sigma公司产品,批号:042K49501.MTT(四甲基噻唑蓝),德国Sigma公司产品,批号:020609.cAMP(环一磷酸腺苷)日本ヤマサ酱油株式会社产品,批号:00207.胚胎牛血清(FBS):由美国Hyclone公司提供,批号:0405.DMEM培养基:美国GIBCO公司产品,批号:0311.干预:NG108-15细胞培养法:含50 mL/L胚牛血清、10 g/L HAT、10 g/L青霉素和链霉素(1:1)的DMEM培养液,置100 mL/L CO2、37℃用的培养箱内培养殖,每隔一天换一次液,4 d传代一次.主要观察指标:利用MTT法、细胞计数法和细胞形态学检测法观察PNS对NG108-15细胞存活率和细胞突起的改变.结果:PNS能增加Aβ25~35片段神经毒性诱导的NG108-15细胞存活率[增殖状态:(311±21)%,分化状态:(113±31)%,P<0.05,F=5.567]、细胞突起率[增殖状态:(25.66±3.65)%,分化状态:(67.97±2.47)%,P<0.05,F=6.471]和细胞突起平均长度[增殖状态:(25.66±3.09)μm,分化状态:(25.66±3.65)μm,P<0.001,F=12.641].结论:PNS具有减轻细胞对Aβ的神经毒性反应和促进细胞突起生长的作用,表明PNS能对老年性痴呆的病理发展有拮抗作用.%BACKGROUND: Although the pathology and

  11. Activity-based protein profiling of secreted cellulolytic enzyme activity dynamics in Trichoderma reesei QM6a, NG14, and RUT-C30

    Energy Technology Data Exchange (ETDEWEB)

    Anderson, Lindsey N.; Culley, David E.; Hofstad, Beth A.; Chauvigne-Hines, Lacie M.; Zink, Erika M.; Purvine, Samuel O.; Smith, Richard D.; Callister, Stephen J.; Magnuson, Jon M.; Wright, Aaron T.

    2013-12-01

    Development of alternative, non-petroleum based sources of bioenergy that can be applied in the short-term find great promise in the use of highly abundant and renewable lignocellulosic plant biomass.1 This material obtained from different feedstocks, such as forest litter or agricultural residues, can yield liquid fuels and other chemical products through biorefinery processes.2 Biofuels are obtained from lignocellulosic materials by chemical pretreatment of the biomass, followed by enzymatic decomposition of cellulosic and hemicellulosic compounds into soluble sugars that are converted to desired chemical products via microbial metabolism and fermentation.3, 4 To release soluble sugars from polymeric cellulose multiple enzymes are required, including endoglucanase, exoglucanase, and β-glucosidase.5, 6 However, the enzymatic hydrolysis of cellulose into soluble sugars remains a significant limiting factor to the efficient and economically viable utilization of lignocellulosic biomass for transport fuels.7, 8 The primary industrial source of cellulose and hemicellulases is the mesophilic soft-rot fungus Trichoderma reesei,9 having widespread applications in food, feed, textile, pulp, and paper industries.10 The genome encodes 200 glycoside hydrolases, including 10 cellulolytic and 16 hemicellulolytic enzymes.11 The hypercellulolytic catabolite derepressed strain RUT-C30 was obtained through a three-step UV and chemical mutagenesis of the original T. reesei strain QM6a,12, 13 in which strains M7 and NG14 were intermediate, having higher cellulolytic activity than the parent strain but less activity and higher catabolite repression than RUT-C30.14 Numerous methods have been employed to optimize the secreted enzyme cocktail of T. reesei including cultivation conditions, operational parameters, and mutagenesis.3 However, creating an optimal and economical enzyme mixture for production-scale biofuels synthesis may take thousands of experiments to identify.

  12. Effects of Nitric Oxide Production Inhibitor Named, NG-Nitro-L-Arginine Methyl Ester (L-NAME, on Rat Mesenchymal Stem Cells Differentiation

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    E Arfaei

    2010-04-01

    Full Text Available Introduction & Objectives: Recently, the findings of some studies have shown that, nitric oxide (NO probably has an important role in differentiation of mesenchymal stem cells to osteoblasts. The aim of the present investigation was to study the effects of nitric oxide production inhibitor named, NG-nitro-L-arginine methyl ester (L-NAME, on rat mesenchymal stem cells differentiation to osteoblasts in vitro. Materials & Methods: This was an experimental study conducted at Hamedan University of Medical Sciences in 2009, in which rat bone marrow stem cells were isolated in an aseptic condition and cultured in vitro. After third passage, the cells were cultured in osteogenic differentiation medium. To study the effects of L-NAME on osteogenic differentiation, the L-NAME was added to the culture medium at a concentration of 125, 250, and 500 μM in some culture plates. During the culture procedure, the media were replaced with fresh ones, with a three days interval. After 28 days of culturing the mineralized matrix was stained using Alizarian red staining method. The gathered data were analyzed by SPSS software version 12 using one way ANOVA. Results: The findings of this study showed that in the presence of L-NAME, differentiation of bone marrow mesenchymal stem cells to osteoblasts was disordered and matrix mineralization significantly decreased in a dose dependent manner. Conclusion: This study revealed that, inhibition of nitric oxide production using L-NAME can prevent the differentiation of rat bone marrow mesenchymal stem cells to osteoblast. The results imply that NO is an important constituent in differentiation of mesenchymal stem cell to osteoblasts.

  13. The Nitric Oxide Synthase Inhibitor NG-Nitro-L-Arginine Methyl Ester Diminishes the Immunomodulatory Effects of Parental Arginine in Rats with Subacute Peritonitis.

    Science.gov (United States)

    Lo, Hui-Chen; Hung, Ching-Yi; Huang, Fu-Huan; Su, Tzu-Cheng; Lee, Chien-Hsing

    2016-01-01

    The combined treatment of parenteral arginine and the nitric oxide synthase inhibitor NG-nitro-L-arginine methyl ester (L-NAME) have been shown to improve liver function and systemic inflammation in subacute peritonitic rats. Here, we investigated the effects of single and combined parenteral arginine and L-NAME treatments on leukocyte and splenocyte immunity. Male Wistar rats were subjected to cecal punctures and were intravenously given total parenteral nutrition solutions with or without arginine and/or L-NAME supplementations for 7 days. Non-surgical and sham-operated rats with no cecal puncture were given a chow diet and parenteral nutrition, respectively. Parenteral feeding elevated the white blood cell numbers and subacute peritonitis augmented the parenteral nutrition-induced alterations in the loss of body weight gain, splenomegaly, and splenocyte decreases. Parenteral arginine significantly increased the B-leukocyte level, decreased the natural killer T (NKT)-leukocyte and splenocyte levels, alleviated the loss in body weight gain and total and cytotoxic T-splenocyte levels, and attenuated the increases in plasma nitrate/nitrite and interferon-gamma production by T-splenocytes. L-NAME infusion significantly decreased NKT-leukocyte level, tumor-necrosis factor (TNF)-alpha production by T-splenocytes and macrophages, and interferon-gamma production by T-leukocytes, monocytes, and T-splenocytes, as well as increased interleukin-6 production by T-leukocytes and monocytes and nitrate/nitrite production by T-leukocytes. Combined treatment significantly decreased plasma nitrate/nitrite, the NKT-leukocyte level, and TNF-alpha production by T-splenocytes. Parenteral arginine may attenuate immune impairment and L-NAME infusion may augment leukocyte proinflammatory response, eliminate splenocyte proinflammatory and T-helper 1 responses, and diminish arginine-induced immunomodulation in combined treatment in subacute peritonitic rats.

  14. Anthraquinones and nitrogen secondary metabolites from the coral-derived symbiotic fungi NG-15 -3 of South China Sea%南海珊瑚共附生真菌 NG -15-3中蒽醌和含氮类次生代谢产物研究

    Institute of Scientific and Technical Information of China (English)

    李秀婷; 刘炳新; 胡谷平; 汪玉梅; 张翠仙

    2016-01-01

    In order to study the secondary metabolites from the coral-derived symbiotic fungi NG-15 -3 of South China Sea,the compounds were isolated by silica gel column chromatography and HPLC.Their structures were identified on the basis of spectral data and physical constant comparison.Four anthraqui-nones and six nitrogen compounds were isolated as preechinulin (1),emodin (2),1-O -methyl emodin (3),physcion (4),carviolin (5),cyclo-(Phe-Gly)(6),cyclo-(Gly-Pro)(7),3H-imidazole-4-carboxylic acid (8),2-methyl-3H-imidazole-4-carboxylic acid (9)and 1H-pyrrole-2-carboxylic acid (10).All the secondary metabolites were firstly acquired from the coral-derived symbiotic fungi NG-15 -3 of South China Sea and compound 1 was a precursors of the monodehydro -2,5-diketopiperazines, which showed varied biological activity.%为了研究南海珊瑚共附生真菌 NG -15-3次生代谢产物,采用硅胶柱层析和 HPLC 等分离手段从乙酸乙酯提取部位分离得到单体化合物,结合现代波谱技术、物理常数对照对单体化合物的结构进行结构鉴定。从其分离得到4个蒽醌类:emodin (2)、1-O -methyl emodin (3)、physcion (4)、carviolin (5)和6个含氮类次生代谢产物:preechinulin (1)、cyclo-(Phe-Gly)(6)、cyclo-(Gly-Pro)(7)、3H-imidazole-4-carboxylic acid (8)、2-methyl-3H-imidazole-4-carboxylic acid (9)和1H-pyrrole-2-carboxylic acid (10)。所有化合物均为首次从该真菌中分离得到,且化合物1为单去氢2,5-二酮哌嗪类化合物的前体物质,具有丰富的生理活性。

  15. Contribuição da densidade do PSA para predizer o câncer da próstata em pacientes com valores de PSA entre 2,6 e 10,0 ng/ml

    Directory of Open Access Journals (Sweden)

    Hugo Alexandre Sócrates de Castro

    2011-08-01

    Full Text Available OBJETIVO: Estudar o perfil dos pacientes submetidos a biópsia prostática, determinando possíveis padrões que, associados aos níveis de PSA entre 2,6 e 10,0 ng/ml, possam levar a uma diminuição de biópsias desnecessárias. MATERIAIS E MÉTODOS: De 2007 a 2009, foi realizado um estudo transversal com 1.282 indivíduos submetidos a biópsia prostática e que apresentavam níveis de PSA entre 2,6 e 10,0 ng/ml. RESULTADOS: A prevalência de câncer foi de 28,6%. Pacientes com câncer eram, em média, mais idosos, com valores de PSA e densidade de PSA mais altos e menor volume da próstata. Na análise da densidade de PSA, os pacientes com câncer tiveram média de 0,31 ng/ml/cc, enquanto nos pacientes com resultado negativo a média foi de 0,10 ng/ml/cc. Utilizando como critério de positividade para câncer o ponto de corte de densidade de PSA de 0,15 ng/ml/cc, obtivemos especificidade de 74% e sensibilidade de 70%. Para aumentar a sensibilidade é preciso reduzir o ponto de corte. Com o valor 0,09 ng/ml/cc, obtivemos sensibilidade de 84% (IC 95%: 80-87% e especificidade de 75% (IC 95%: 72-78%. CONCLUSÃO: O uso sistemático da densidade de PSA na indicação de prosseguimento da investigação do paciente com biópsia poderia reduzir a quantidade de procedimentos desnecessários.

  16. Perfil nutricional e fatores associados à ocorrência de desnutrição entre crianças indígenas Kaingáng da Terra Indígena de Mangueirinha, Paraná, Brasil Nutritional profile and factors associated with malnutrition in Kaingáng children on the Mangueirinha Indigenous Reserve, Paraná State, Brazil

    Directory of Open Access Journals (Sweden)

    Adriana Masiero Kühl

    2009-02-01

    Full Text Available Este estudo objetivou avaliar o estado nutricional de crianças indígenas e verificar os fatores associados à ocorrência de agravos nutricionais. Foram avaliadas 141 crianças Kaingáng de 0 a 60 meses de idade da Terra Indígena de Mangueirinha, Paraná, Brasil, por meio das medidas de peso e altura ou comprimento. Realizaram-se entrevistas com a aplicação de um questionário sobre condições materno-infantis e sócioeconômicas. Segundo os parâmetros da Organização Mundial da Saúde (2006, 24,8% das crianças apresentavam déficit de altura para idade (AI, 9,2% baixo peso para idade (PI, 2,1% baixo peso para altura (PA e 2,1% baixo peso segundo o índice de massa corporal para idade (IMCI. De acordo com o NCHS (1977, 19,9% das crianças apresentaram baixa AI, 9,2% baixo PI e 1,4% baixo PA. O sobrepeso chegou a 6,4%, segundo o IMCI. O baixo peso ao nascer e os materiais utilizados na construção das paredes das casas estiveram estatisticamente associados com déficits antropométricos. Este estudo mostra que as crianças Kaingáng estão inseridas em um contexto marcado por precárias condições de vida, que estão associadas com indicadores desfavoráveis do estado nutricional.The goal of this study was to evaluate the nutritional status of indigenous children and to investigate factors associated with nutritional deficits. Weight and height measurements were obtained for 141 Kaingáng children from 0 to 5 years of age living on the Mangueirinha Indigenous Reserve in Paraná State, Brazil. Data on maternal and infant conditions and socioeconomic characteristics were also gathered through face-to-face interviews. Based on World Health Organization criteria (2006, 24.8% of the children presented low height-for-age (HA, 9.2% low weight-for-age (WA, 2.1% low weight-for-height (WH, and 2.1% low weight according to body mass index for age (BMIA. Based on NCHS criteria (1977, 19.9% of the children presented low HA, 9.2% low WA, and 1

  17. Organização e qualidade da assistência à saúde dos índios Kaingáng do Rio Grande do Sul, Brasil Organization and quality of health care for Kaingáng Indians in Rio Grande do Sul, Brazil

    Directory of Open Access Journals (Sweden)

    Yara Hahr Marques Hökerberg

    2001-03-01

    Full Text Available Este estudo pretende avaliar o atendimento à saúde prestado aos índios Kaingáng do Rio Grande do Sul. Os óbitos evitáveis por assistência básica de saúde dos indígenas, ocorridos entre 1985 e 1995, foram comparados aos óbitos do Estado do Rio Grande do Sul. Os dados secundários relativos aos serviços de saúde foram complementados por entrevistas realizadas em campo com lideranças indígenas e com funcionários das instituições envolvidas na assistência à saúde do índio. Foi utilizado o Sistema de Informações Geográficas, visando relacionar a distribuição dos óbitos ao grau de acesso aos serviços de saúde. As áreas indígenas Kaingáng estão ligadas por estradas pavimentadas a municípios que contam com, pelo menos, um centro de saúde, ou ainda, hospitais de pequeno porte. O segundo nível de atenção situa-se em Palmeiras das Missões e Frederico Westphallen e o terceiro nível, em Passo Fundo. O que diferencia estas áreas indígenas do restante do Estado do Rio Grande do Sul é a quantidade de óbitos evitáveis pela assistência básica de saúde, tais como as causas mal definidas, a desnutrição, a tuberculose e o câncer cérvico-uterino.This study assesses the health care provided to Kaingáng Indians in Rio Grande do Sul, Brazil. Deaths preventable by primary health care among the Indians and occurring from 1985 to 1995 were compared to the same rates for the State of Rio Grande do Sul as a whole. Secondary data on health care services were supplemented by field interviews with indigenous leaders and with employees from participating institutions. A Geographic Information System (GIS was used to correlate distribution of deaths and access to health services. The Kaingáng settlements are connected by paved roads to counties with at least a public health clinic or even a small hospital in some cases. Secondary referrals are treated in Palmeiras das Missões and Frederico Westphallen and tertiary care is

  18. Identification and Antagonistic Activity of Endophytic Bacterial Strain NG14 Isolated from the Fruits of Paracel Islands Noni (Morinda citrifolia L.)%一株西沙群岛野生诺尼内生细菌NG14的分类鉴定及拮抗活性

    Institute of Scientific and Technical Information of China (English)

    刘洋; 李金霞; 姚粟; 张明娟; 陈建国; 程池

    2014-01-01

    植物的内生菌与植物生长健康及其功效性成分产生具有一定的相关性.以分离自我国西沙群岛野生诺尼成熟果实的内生细菌菌株NG14为对象,利用形态学观察,生理生化特征鉴定及系统发育学分析,对其菌种的分类地位进行研究,将其鉴定为多黏类芽孢杆菌(Paenibacillus polymyxa);并通过选择多种植物及人体病原菌作为生防对象进行拮抗试验,发现了该菌株具有广谱且良好的拮抗病原菌活性.

  19. Lead determination at ng/mL level by flame atomic absorption spectrometry using a tantalum coated slotted quartz tube atom trap.

    Science.gov (United States)

    Demirtaş, İlknur; Bakırdere, Sezgin; Ataman, O Yavuz

    2015-06-01

    Flame atomic absorption spectrometry (FAAS) still keeps its importance despite the relatively low sensitivity; because it is a simple and economical technique for determination of metals. In recent years, atom traps have been developed to increase the sensitivity of FAAS. Although the detection limit of FAAS is only at the level of µg/mL, with the use of atom traps it can reach to ng/mL. Slotted quartz tube (SQT) is one of the atom traps used to improve sensitivity. In atom trapping mode of SQT, analyte is trapped on-line in SQT for few minutes using ordinary sample aspiration, followed by the introduction of a small volume of organic solvent to effect the revolatilization and atomization of analyte species resulting in a transient signal. This system is economical, commercially available and easy to use. In this study, a sensitive analytical method was developed for the determination of lead with the help of SQT atom trapping flame atomization (SQT-AT-FAAS). 574 Fold sensitivity enhancement was obtained at a sample suction rate of 3.9 mL/min for 5.0 min trapping period with respect to FAAS. Organic solvent was selected as 40 µL of methyl isobutyl ketone (MIBK). To obtain a further sensitivity enhancement inner surface of SQT was coated with several transition metals. The best sensitivity enhancement, 1650 fold enhancement, was obtained by the Ta-coated SQT-AT-FAAS. In addition, chemical nature of Pb species trapped on quartz and Ta surface, and the chemical nature of Ta on quartz surface were investigated by X-ray photoelectron spectroscopy (XPS) and Raman Spectroscopy. Raman spectrometric results indicate that tantalum is coated on SQT surface in the form of Ta2O5. XPS studies revealed that the oxidation state of Pb in species trapped on both bare and Ta coated SQT surfaces is +2. For the accuracy check, the analyses of standard reference material were performed by use of SCP SCIENCE EnviroMAT Low (EU-L-2) and results for Pb were to be in good agreement with

  20. Dynamic contrast enhanced MRI detects early response to adoptive NK cellular immunotherapy targeting the NG2 proteoglycan in a rat model of glioblastoma.

    Directory of Open Access Journals (Sweden)

    Cecilie Brekke Rygh

    Full Text Available There are currently no established radiological parameters that predict response to immunotherapy. We hypothesised that multiparametric, longitudinal magnetic resonance imaging (MRI of physiological parameters and pharmacokinetic models might detect early biological responses to immunotherapy for glioblastoma targeting NG2/CSPG4 with mAb9.2.27 combined with natural killer (NK cells. Contrast enhanced conventional T1-weighted MRI at 7±1 and 17±2 days post-treatment failed to detect differences in tumour size between the treatment groups, whereas, follow-up scans at 3 months demonstrated diminished signal intensity and tumour volume in the surviving NK+mAb9.2.27 treated animals. Notably, interstitial volume fraction (ve, was significantly increased in the NK+mAb9.2.27 combination therapy group compared mAb9.2.27 and NK cell monotherapy groups (p = 0.002 and p = 0.017 respectively in cohort 1 animals treated with 1 million NK cells. ve was reproducibly increased in the combination NK+mAb9.2.27 compared to NK cell monotherapy in cohort 2 treated with increased dose of 2 million NK cells (p<0.0001, indicating greater cell death induced by NK+mAb9.2.27 treatment. The interstitial volume fraction in the NK monotherapy group was significantly reduced compared to mAb9.2.27 monotherapy (p<0.0001 and untreated controls (p = 0.014 in the cohort 2 animals. NK cells in monotherapy were unable to kill the U87MG cells that highly expressed class I human leucocyte antigens, and diminished stress ligands for activating receptors. A significant association between apparent diffusion coefficient (ADC of water and ve in combination NK+mAb9.2.27 and NK monotherapy treated tumours was evident, where increased ADC corresponded to reduced ve in both cases. Collectively, these data support histological measures at end-stage demonstrating diminished tumour cell proliferation and pronounced apoptosis in the NK+mAb9.2.27 treated tumours compared to the other

  1. Effect of a nitric oxide synthase inhibitor NG-nitro-L-arginine methyl ester on invasion of human colorectal cancer cell line SL-174T

    Institute of Scientific and Technical Information of China (English)

    Li-Bo Yu; Xin-Shu Dong; Wen-Zhou Sun; Dong-Lu Zhao; Yue Yang

    2005-01-01

    AIM: To investigate the effect and mechanism of action of the nitric oxide synthase (NOS) inhibitor NG-nitro-L-arginine methyl ester (L-NAME) on invasion and metastasis of human colorectal cancer cell line SL-174T.METHODS: Human colorectal cancer cell line SL-174T was cultured and treated separately with four different dosages of L-NAME for 72 h. Nitric oxide (NO) production was measured with Griess reagent. The effect of L-NAME on invasion and migration of SL-174T cells were evaluated by using Transwell chambers attached with polycarbonate filters and reconstituted basement membrane (Matrigel).RT-PCR was performed to determine the mRNA levels of matrix metalloproteinase-2 (MMP-2) and tissue inhibitor metalloproteinase-2 (TIMP-2).RESULTS: L-NAME could significantly inhibit NO production of SL-174T in a dose-dependent manner. After being treated for 72 h with 0.2, 0.4, 0.8, and 1.0 mmol/L LNAME, respectively, the ability of the L-NAME treated SL174T cells to invade the reconstituted basement membrane decreased significantly (t = 8.056, P<0.05;t= 14.467, P<0.01;t= 27.785, P<0.01;and t= 29.405,P<0.01, respectively) and the inhibition rates were 10.29%,19.62%, 34.08%, and 42.23%, respectively. Moreover,L-NAME could inhibit migration of SL-174T cells, and the inhibition rates were 20.76%, 24.95%, 39.43%, and 46.85% for L-NAME at 0.2, 0.4, 0.8, and 1.0 mmol/L,respectively (t = 15.116, P<0.01). In addition, after treatment with L-NAME, expression of MMP-2 mRNA was significantly decreased (t = 71.238, P<0.01) and that of TIMP-2 mRNA was markedly increased (t = -13.020,P<O.01).CONCLUSION: L-NAME exerts anti-invasive and antimetastatic effects on SL-174T cell line via downregulating MMP-2 mRNA expression and upregulating TIMP-2 mRNA expression.

  2. pg-ng级Os同位素热表面电离质谱高精度分析测试技术%High Precise Isotopic Measurements of pg-ng Os by Negative Ion Thermal Ionization Mass Spectrometry

    Institute of Scientific and Technical Information of China (English)

    李超; 杨雪; 赵鸿; 周利敏; 杜安道; 李欣尉; 屈文俊

    2015-01-01

    随着Os同位素在地质学领域定年与示踪的广泛应用,对分析测试精度和检出限提出了更高的要求。本文利用热表面电离质谱负离子模式( HTIMS)对OsO3-进行测定,通过试剂纯化、器皿清洗等前处理步骤的优化保证了超低的化学流程空白和获得灵敏而稳定的仪器测量信号,建立了高精度pg-ng级Os同位素分析测试方法。采用逐级剥谱法扣除氧同位素干扰,采用内标迭代法按照指数规律对仪器的质量歧视效应进行校正,全流程Os空白为0.41 pg,1 pg的190 Os计数约85000 cps,质量分馏系数在0.1%以内。pg级Os采用离子计数器动态跳峰扫描模式进行测量,187 Os/188 Os值测量精度小于在0.2%以内;ng级Os采用法拉第杯静态接收模式采集信号,187 Os/188 Os值测量精度在0.005%以内。经国际上Os同位素标准溶液H2、JMC以及国家一级Re-Os同位素标准物质JCBY的验证,分析精度达到了国际同类实验室先进水平。本方法具有低空白、高灵敏度、高精度的特点,可为地质学研究提供高精度Os同位素数据。%Higher analytical precision and lower detection limit are required in geological dating and tracing,due to the wide application of Os. In this study,high-precision determination of pg-ng Os by Hegative Ion Thermal Ionization Mass Spectrometry( HTIMS ) was established by reagent purification,vessel cleaning and chemical preparation to ensure the ultra-low blank and obtain sensitive and stable signals. Isobaric oxygen interferences are corrected by stepwise spectrum stripping,and mass discrimination effect is corrected by internal standard iteration. The total procedural blank of Os is 0. 41 pg,the signal of 1 pg 190 Os is about 85000 cps,and the mass fractionation factor is less than 0 . 1%. Pg level of Os is measured by ion counter dynamic jumping scan mode and the measurement precision of 187 Os/ 188 Os is less than 0 . 2%. ng level of Os

  3. Effect of electric acupuncture on the expression of NgR in the cerebral cortex,the medulla oblongata,and the spinal cord of hypertensive rats after cerebral infarction

    Institute of Scientific and Technical Information of China (English)

    谭峰

    2014-01-01

    Objective To observe the effect of electric acupuncture(EA)on the Nogo receptors(NgR)protein expression in the cerebral cortex,the medulla oblongata,and the spinal cord of cerebral ischemia-reperfusion(I/R)stroke-prone renovascular hypertensive rats(RHRSP)with middle cerebral artery occlusion(MCAO)at different time points,and to investigate its possible mecha-

  4. Worldwide susceptibility rates of Neisseria gonorrhoeae isolates to cefixime and cefpodoxime: a systematic review and meta-analysis.

    Directory of Open Access Journals (Sweden)

    Rui-xing Yu

    Full Text Available BACKGROUND: Neisseria gonorrhoeae (NG infection is a serious public health problem. The third-generation extended-spectrum cephalosporins (ESCs have been used as the first-line treatment for NG infection for almost three decades. However, in recent years, treatment failures with the oral third-generation ESCs have been reported worldwide. This study aimed to estimate worldwide susceptibility rates of NG to cefixime and cefpodoxime by analyzing data from all relevant published studies. METHODOLOGY/PRINCIPAL FINDINGS: Two researchers independently searched five databases to identify studies on susceptibilities of NG to cefixime and cefpodoxime published between January 1, 1984 and October 15, 2012. A fixed-effect model was used to perform group analysis, and a χ2 test was employed to make subgroup comparison. Publication bias was assessed with the Begg rank correlation test. The pooled susceptibility rate of NG isolates to cefixime was 99.8% (95% CI: 99.7%-99.8%. The cefixime susceptibility rate of NG isolates from men was significantly lower than that from patients without information of gender or from men and women; the susceptibility rate of NG isolates from Asia was significantly lower than that from other continents; and the susceptibility rate of NG isolates collected before or during 2003 was significantly higher than that after 2003. The pooled susceptibility rate of NG isolates to cefpodoxime was 92.8% (95% CI: 89.0%-95.3%, which was lower than that to cefixime (92.8% vs. 99.8%, χ2 = 951.809, P<0.01. CONCLUSIONS: The susceptibility rate of NG isolates to cefixime varied with the gender of patients and geographical location from which NG isolates were collected, and declined with time. The reported lower susceptibility rate of NG isolates to cefixime and associated treatment failures, as well as the emergence of NG strains with cephalosporin resistance call for the more effective control of NG infection and the development of new

  5. Is it necessary to perform control diagnostic 131I whole body scan after remnant ablation in differentiated thyroid carcinoma patients who have stimulated Tg levels under 2 ng/ml?

    International Nuclear Information System (INIS)

    Full text of publication follows. Introduction: the aim of this retrospective study is to evaluate whether diagnostic I131 whole body scan (DWBS) performed 6-12 months after thyroid remnant ablation is necessary or not in differentiated thyroid carcinoma (DTC) patients with thyroglobulin (Tg) levels under 2 ng/ml and negative anti-Tg antibodies. Materials and methods: the study included 812 DTC patients undergoing the first control DWBS with Tg levels under 2 ng/ml and negative anti-Tg antibodies in the hypothyroid state, 6 months after post-surgical radio-ablation of residual thyroid tissue. Second DWB was performed 18 months after ablative I131 treatment (AIT) to 572 patients who had negative first control DWBS. These 572 patients had a stimulated Tg level under 2 ng/ml at the 18. month control. Results: the first control DWBS, 6 months after thyroid ablation, was negative in 789 (97.2%) patients and was positive for minimal residual uptake in the thyroid bed in 23 (2.8%) patients. 9 of the 23 patients with residual thyroid bed uptake, received a second dose of radioiodine therapy for complementary ablation of residual thyroid. 14 patients were not retreated. Second DWBS performed 18 months after AIT, was negative in all patients. Conclusion: our data suggest that in patients with stimulated Tg level under 2 ng/ml, DWBS performed 6 months after AIT, is informative only in minority of patients for residual uptake in the thyroid bed which is usually clinically not relevant. Therefore, we suggest that the DWBS may be avoided in patients with stimulated Tg level under 2 ng/ml. (authors)

  6. Analysis of vesicle fluid following the sting of the lionfish Pterois volitans.

    Science.gov (United States)

    Auerbach, P S; McKinney, H E; Rees, R S; Heggers, J P

    1987-01-01

    Fluid aspirated from blisters following a lionfish (Pterois volitans) sting was analyzed utilizing combined capillary column gas chromatography and negative ion chemical ionization mass spectrometry. Analysis for prostaglandin F2 alpha demonstrated 16.91 ng/ml, for prostaglandin E2 0.143 ng/ml, for 6-keto-prostaglandin F1 alpha less than 0.1 ng/ml (nondetectable) and for thromboxane B2 1.65 ng/ml. Platelet aggregation studies showed that blister fluid caused aggregation of isolated platelets only, which was inhibited by heat treatment or by the presence of normal donor plasma. PMID:3438924

  7. 蛋白磷酸酯酶-1和-2A抑制剂对NG108-15细胞tau蛋白磷酸化的影响%EFFECT OF PROTEIN PHOSPHATASE-1 AND 2A INHIBITOR ON TAU PROTEIN PHOSPHORYLATION OF NG108-14 CELL

    Institute of Scientific and Technical Information of China (English)

    朱粹青; 曹小定

    2002-01-01

    Alzheimer痴呆(AD)的主要脑病理变化之一为由超磷酸化的tau蛋白组成的神经原纤维缠结(Neurofibrillary tangle,NFT).AD的Tau蛋白异常磷酸化与蛋白磷酸酯酶(PP)-2A和-1缺陷有关.本文首先用免疫印迹法显示NG含两大组分的tau蛋白.MTT方法观察到PP-2A和PP-1抑制剂冈田酸(Okadaic acid,OA)处理NG108-14细胞能导致细胞代谢明显下降,同时免疫印迹法显示OA能导致的NG细胞Tau蛋白磷酸化.该研究为建立AD样蛋白磷酸酯酶缺陷引起的tau蛋白磷酸化的细胞模型奠定了基础.

  8. 骨髓间充质干细胞移植影响慢性脑缺血大鼠认知功能及海马区Nogo-A 、NgR 的表达%Transplantation of bone marrow mesenchymal stem cells improves cognitive function of rats with chronic cerebral ischemia and decreases Nogo-A and NgR protein expression in the hippocampus

    Institute of Scientific and Technical Information of China (English)

    王燕琳; 闫禹; 宋波; 张惠莉; 龚光明; 陈思; 方慧; 许予明

    2012-01-01

    BACKGROUND: Transplantation of bone marrow mesenchymal stem cells can decrease Nogo-A and NgR expression in rats with spinal cord injury and cerebral infarction. OBJECTIVE: To investigate the effects of transplantation of bone marrow mesenchymal stem cells on cognitive function of rats with chronic cerebral ischemia and Nogo-A and NgR protein expression in the hippocampus. METHODS: Thi rty Sprague-Dawley rats were randomly divided into sham-surgery, model and bone marrow mesenchymal stem cells groups. Rats in the latter two groups were established into model of chronic cerebral ischemia by permanent ligation of bilateral common carotid artery. In the bone marrow mesenchymal stem cells group, at 7 days after chronic cerebral ischemia induction, bone marrow mesenchymal stem cells (1×107) isolated by ficoll density gradient centrifugation were transfused into rats via the tailvein. RESULTS AND CONCLUSION: Compared with sham-surgery group, the average escape latency was significantly shortened (P < 0.01), the number that rats passed through the platform was significantly increased (P < 0.05), and Nogo-A and NgR expression in the hippocampal ti ssue was significantly decreased (P < 0.05) in the bone marrow mesenchymal stem cells group. These findings suggest that transplantation of bone marrow mesenchymal stem cells protects spatial learning and memory abilities of rats with chronic cerebral ischemia. This effect is possibly related to decreased Nogo-A and NgR protein expression.%背景:骨髓间充质干细胞移植可降低脊髓损伤及脑梗死大鼠的Nogo-A 及NgR 的表达.目的:观察骨髓间充质干细胞移植对慢性脑缺血大鼠认知功能及海马区Nogo-A 及NgR 蛋白的表达.方法:将30 只SD大鼠随机等分为假手术组、模型组及骨髓间充质干细胞组,后2 组采用双侧颈总动脉永久结扎法建立大鼠慢性脑缺血模型,骨髓间充质干细胞组在建模7 d后尾静脉注射移植用ficoll密度梯度离心法分

  9. ng i jazzgenren på gymnasienivå : En intervjustudie av fyra sångpedagogers syn på undervisning och arbetssätt inom genreområdet jazz

    OpenAIRE

    Waite, Nellie

    2013-01-01

    Syftet med studien är att få ökad insikt i hur sånglärare med utgångspunkt i kursplanens mål om genrebreddning hanterar genreområdet jazzsång i sångundervisningen på gymnasieskolans estetiska program. Genom att intervjua fyra sångpedagoger verksamma på gymnasiets estetiska program har jag tagit reda på vad som enligt dem kan betecknas som ett genreenligt sound eller genrespecifika egenskaper speciellt viktiga för jazzsång, hur dessa kunskaper kan förmedlas till eleverna samt hur sångpedagoger...

  10. Representasi Gaya Hidup Wanita Metropolis Dalam Novel (Analisis Wacana Teun A. Van Dijk Mengenai Representasi Gaya Hidup Wanita Metropolis Dalam Novel Indiana Chronicles “Blues” Karya Clara Ng)

    OpenAIRE

    Rahmatika, Khairia

    2010-01-01

    Penelitian ini berjudul “Representasi Gaya Hidup Wanita Dalam Novel” (Analisis Wacana Teun A. van Dijk Mengenai Representasi Gaya Hidup Wanita Metropolis Dalam Novel Indiana Cronicle Blues Karya Clara Ng). Gaya hidup adalah pola hidup seseorang di dunia yang merupakan ciri sebuah dunia modern, atau yang biasa juga disebut modernitas. Kebutuhan akan status dan terpaan budaya asing ini mengakibatkan merebaknya gaya hidup metropolis yang cenderung permisif dan mengedepankan kemewahan daripada ke...

  11. Three-step laser excitation of the 6p3/2ns, nd, ng autoionizing Rydberg levels via the 6p5f 1/2[5/2]2 level of lead

    Science.gov (United States)

    Ahad, A.; Nadeem, A.; Bhatti, S. A.; Baig, M. A.

    2005-03-01

    Odd parity autoionizing Rydberg levels of atomic lead in the energy region above the 6p1/2 ionization threshold have been investigated using three-step laser excitation in conjunction with an atomic beam apparatus. The 6p3/2ns (J = 1, 2), 6p3/2nd (J = 1, 2, 3) and 6p3/2ng (J = 2, 3) levels have been observed from the 6p5f 1/2[5/2]2 intermediate level. Energy values and FWHM of forty levels belonging to the 6p3/2ns, 6p3/2nd and 6p3/2ng configurations are presented. Six levels based on the 6p3/2ng (5, 13 ≤n ≤15) configurations and three levels attached to the 6p3/28d configuration are reported for the first time. The present study of the low-lying autoionizing levels attached to the 6p3/25g (J = 2, 3) configuration completes the series adjacent to the 6p1/2 limit.

  12. Iron chelation therapy with deferasirox in patients with aplastic anemia: a subgroup analysis of 116 patients from the EPIC trial

    DEFF Research Database (Denmark)

    Lee, Jong Wook; Yoon, Sung-Soo; Shen, Zhi Xiang;

    2010-01-01

    The prospective 1-year Evaluation of Patients' Iron Chelation with Exjade (EPIC) study enrolled a large cohort of 116 patients with aplastic anemia; the present analyses evaluated the efficacy and safety of deferasirox in this patient population. After 1 year, median serum ferritin decreased...... significantly from 3254 ng/mL at baseline to 1854 ng/mL (P chelation-naive (3229-1520 ng/mL; P chelated (3263-2585 ng/mL; P = .21, last-observation-carried-forward analysis) patients and were reflective of dose...

  13. Criticality assessment of terrorism related events at different time scales TENSOR clusTEriNg terroriSm actiOn pRediction

    OpenAIRE

    Sormani, R

    2016-01-01

    Law Enforcement Agencies (LEAs) are nowadays taking advantage of a wide range of information and intelligence sources (e.g., human intelligence (HUMINT), open source intelligence (OSINT), image analysis (IMINT)) to anticipate potential terroristic actions. Urban environments are nowadays associated with a wide range of vulnerabilities, which create fertile ground for terrorists planning actions against assets and/or citizens. These vulnerabilities stem from the characteristics of the urban en...

  14. Aerodynamická analýza a návrh úprav podvozkové gondoly letounu L 410 NG

    OpenAIRE

    Pukl, Marek

    2013-01-01

    Tato diplomová práce se zabývá analýzou proudění kolem podvozkové gondoly letounu L 410 a její následnou aerodynamickou optimalizací. V první části je v rámci kalibrační úlohy zpracována známá geometrie, jejíž parametry byly ověřeny tunelovým měřením, následující části pak zahrnují návrh vlastní optimální geometrie, tvorbu výpočetní sítě a její výpočet a konečné zhodnocení výsledků. This diploma thesis deals with the flow analysis around the landing gear nacelle of L 410 and with its follo...

  15. Evaluation of PCA3 and multiparametric MRI’s: collective benefits before deciding initial prostate biopsy for patients with PSA level between 3-10ng/mL

    Directory of Open Access Journals (Sweden)

    Sezgin Okcelik

    2016-06-01

    Full Text Available ABSTRACT Objective To analyze the contribution of multiparametric MRI and PCA3 assay, pre- decision of initial biopsy in PSA level between 3-10 ng/mL patients with normal digital rectal examination(DRE. Materials and Methods PSA level 3-10 ng/mL ,patients, with normal DRE results and no previous prostate biopsy history, were included in this study. Each patient underwent multiparametric MRI one week before biopsy. Urine sample taking for PCA3 examination preceded the biopsy. Systematic and targeted biopsies were conducted. Patients with high PSA levels were seperated into two groups as: high PCA3 scored and low PCA3 scored. Then each group was divided into two sub-groups as: MRI lesion positive and negative. Tumor incidence, positive predictive values(PPV and negative predictive values(NPV were calculated. Results 53 patients were included between February 2013 and March 2014.Mean age 61.22 ± 1.06. Mean PSA value 5.13 ± 0.19 ng / mL. Mean PCA3 score 98.01 ± 23.13 and mean prostate size was 48.96 ± 2.67 grams. Fourty nine patients had both PCA3 score and multiparametric MRI. The PCA3’s PPV value was 58.33%. If multiparametric MRI lesions are added to high PCA3 scores , the PPV appears to elevate to 91.66%. NPV of PCA3 was 96%. NPV was 95% when there was no lesion in the multiparametric MRI with low PCA3 scores. Sensitivity was 91.66% , specificity was 95% respectively. Conclusion Adding multimetric MRI can also support biopsy decision for patients with high PCA3 value. When PCA3 value is low, patients can be survailled without any need to take a MRI.

  16. Extended use of Prostate Health Index and percentage of [-2]pro-prostate-specific antigen in Chinese men with prostate specific antigen 10–20 ng/mL and normal digital rectal examination

    Science.gov (United States)

    Chiu, Peter Ka-Fung; Teoh, Jeremy Yuen-Chun; Lee, Wai-Man; Yee, Chi-Hang; Chan, Eddie Shu-Yin; Hou, See-Ming

    2016-01-01

    Purpose We investigated the extended use of Prostate Health Index (PHI) and percentage of [-2]pro-prostate-specific antigen (%p2PSA) in Chinese men with prostate-specific antigen (PSA) 10–20 ng/mL and normal digital rectal examination (DRE). Materials and Methods All consecutive Chinese men with PSA 10–20 ng/mL and normal DRE who agreed for transrectal ultrasound (TRUS)-guided 10-core prostate biopsy were recruited. Blood samples were taken immediately before TRUS-guided prostate biopsy. The performances of total PSA (tPSA), %free-to-total PSA (%fPSA), %p2PSA, and PHI were compared using logistic regression, receiver operating characteristic, and decision curve analyses (DCA). Results From 2008 to 2015, 312 consecutive Chinese men were included. Among them, 53 out of 312 (17.0%) men were diagnosed to have prostate cancer on biopsy. The proportions of men with positive biopsies were 6.7% in PHI55 (chi-square test, pbiopsy was 0.64. Adding %p2PSA and PHI to the base model improved the AUC to 0.79 (pbiopsy rates of Gleason 7 or above prostate cancers were 2.2% for PHI55 (chi-square test, pbiopsies could be avoided. Conclusions Both PHI and %p2PSA performed well in predicting prostate cancer and high grade prostate cancer. The use of PHI and %p2PSA should be extended to Chinese men with PSA 10–20 ng/mL and normal DRE. PMID:27617315

  17. Contato, epidemias e corpo como agentes de transformação: um estudo sobre a AIDS entre os Índios Xokléng de Santa Catarina, Brasil

    Directory of Open Access Journals (Sweden)

    Flávio Braune Wiik

    2001-03-01

    Full Text Available A partir da análise dos episódios de AIDS que acometeram os Índios Xokléng em 1988, o presente artigo visa referir fenômenos de doença a rupturas e transformações socioculturais ocorridas nesse universo com base na história de contato com a sociedade abrangente. A interpretação e a análise da relação estabelecida são feitas à luz de teorias antropológicas acerca da centralidade que corpo, corporalidade e processos corporais degenerativos ocupam nas sociedades indígenas brasileiras. Propõe-se que corpo, sociedade e agentes macroconjunturais são articulados pela práxis; por isso, devem ser relacionados nos estudos socio-antropológicos concernentes aos fenômenos de doença. O artigo traz a descrição sucinta das epidemias advindas com o contato, tentando vinculá-las aos contextos históricos específicos que o marcaram. Categorias da etnomedicina, cosmologia e corporalidade Xokléng são associadas à organização social e aos casos de AIDS. Estes são apresentados tendo, como enfoque principal, a ligação estabelecida entre seu advento e as transformações no universo Xokléng em função da construção da Barragem Norte junto a sua Terra.

  18. Contato, epidemias e corpo como agentes de transformação: um estudo sobre a AIDS entre os Índios Xokléng de Santa Catarina, Brasil

    Directory of Open Access Journals (Sweden)

    Wiik Flávio Braune

    2001-01-01

    Full Text Available A partir da análise dos episódios de AIDS que acometeram os Índios Xokléng em 1988, o presente artigo visa referir fenômenos de doença a rupturas e transformações socioculturais ocorridas nesse universo com base na história de contato com a sociedade abrangente. A interpretação e a análise da relação estabelecida são feitas à luz de teorias antropológicas acerca da centralidade que corpo, corporalidade e processos corporais degenerativos ocupam nas sociedades indígenas brasileiras. Propõe-se que corpo, sociedade e agentes macroconjunturais são articulados pela práxis; por isso, devem ser relacionados nos estudos socio-antropológicos concernentes aos fenômenos de doença. O artigo traz a descrição sucinta das epidemias advindas com o contato, tentando vinculá-las aos contextos históricos específicos que o marcaram. Categorias da etnomedicina, cosmologia e corporalidade Xokléng são associadas à organização social e aos casos de AIDS. Estes são apresentados tendo, como enfoque principal, a ligação estabelecida entre seu advento e as transformações no universo Xokléng em função da construção da Barragem Norte junto a sua Terra.

  19. Comparison of the Abbott m2000 RealTime CT Assay and the Cepheid GeneXpert CT/NG Assay to the Roche Amplicor CT Assay for Detection of Chlamydia trachomatis in Ocular Samples from Tanzania

    OpenAIRE

    Dize, Laura; West, Sheila; Williams, James A; Van Der Pol, Barbara; Quinn, Thomas C.; Gaydos, Charlotte A.

    2013-01-01

    The GeneXpert CT/NG assay (GeneXpert) and the Abbott m2000 RealTime CT (m2000) assay were compared to Amplicor for detecting ocular Chlamydia trachomatis. Discordant specimens were tested by the Aptima CT assay. The m2000 assay sensitivity was 100% (95% confidence interval [CI], 90% to 100%), and specificity was 98.46% (95% CI, 95.2% to 99.2%); GeneXpert sensitivity was 100% (95% CI, 90% to 100%), and specificity was 100% (95% CI, 98.1% to 100%). The m2000 and GeneXpert assays appear to perfo...

  20. Effect of Kınesıotapıng and Knee Brace on Functıonal Performance in Recreatıonal Athletes

    Science.gov (United States)

    Ulusoy, Burak; İldiz, Bülent; Tunay, Volga Bayrakçı

    2014-01-01

    Objectives: Kinesiotaping is a popular taping method that is used for both therapeutic and performance enchancement purposes. Knee braces are widely used for prevention in sport injuries but their performance effectiveness is still controversial. The aim of this study was to determine whether kinesiotape or brace was more effective on functional performance. Methods: A total twenty male recreational football players (Mean±Standart Deviation (SD) age: 22.5±0.68 years, height: 175.15±3.37 cm, body weight: 74.52±12.41 kg), voluntarily participated in this study. Participants were tested with kinesiotape, with brace and without kinesiotape and brace. Tests were applied one day after patellar kinesiotaping (correction technique). Balance property measured with Modified Y balance Test (dynamic test), agility measured by T test, muscle strength and anaerobic power assessed by vertical jump and triple hop tests. Wilcoxon signed rank test was employed for determining the statistical significance of tests with kinesiotape, with brace and without kinesiotape and brace. Results: In analysis; There were statistically significant differences found in Triple hop test with kinesiotaping and without kinesiotaping and brace, in T test with bracing and kinesiotaping, in vertical jump with kinesiotaping and without kinesiotaping and brace (pkinesiotaping in all tests) No statistically significant difference was found in modified Y balance test all groups (p> 0.05). Conclusion: Consequently, kinesiotaping had positive effects on agility and muscle strength but had no effects on balance in football players. On the other hand, brace had no effects on functional performance tests.

  1. Effect of affordable technology on physical activity levels and mobility outcomes in rehabilitation: a protocol for the Activity and MObility UsiNg Technology (AMOUNT) rehabilitation trial

    Science.gov (United States)

    Hassett, Leanne; van den Berg, Maayken; Lindley, Richard I; Crotty, Maria; McCluskey, Annie; van der Ploeg, Hidde P; Smith, Stuart T; Schurr, Karl; Killington, Maggie; Bongers, Bert; Howard, Kirsten; Heritier, Stephane; Togher, Leanne; Hackett, Maree; Treacy, Daniel; Dorsch, Simone; Wong, Siobhan; Scrivener, Katharine; Chagpar, Sakina; Weber, Heather; Pearson, Ross; Sherrington, Catherine

    2016-01-01

    Introduction People with mobility limitations can benefit from rehabilitation programmes that provide a high dose of exercise. However, since providing a high dose of exercise is logistically challenging and resource-intensive, people in rehabilitation spend most of the day inactive. This trial aims to evaluate the effect of the addition of affordable technology to usual care on physical activity and mobility in people with mobility limitations admitted to inpatient aged and neurological rehabilitation units compared to usual care alone. Methods and analysis A pragmatic, assessor blinded, parallel-group randomised trial recruiting 300 consenting rehabilitation patients with reduced mobility will be conducted. Participants will be individually randomised to intervention or control groups. The intervention group will receive technology-based exercise to target mobility and physical activity problems for 6 months. The technology will include the use of video and computer games/exercises and tablet applications as well as activity monitors. The control group will not receive any additional intervention and both groups will receive usual inpatient and outpatient rehabilitation care over the 6-month study period. The coprimary outcomes will be objectively assessed physical activity (proportion of the day spent upright) and mobility (Short Physical Performance Battery) at 6 months after randomisation. Secondary outcomes will include: self-reported and objectively assessed physical activity, mobility, cognition, activity performance and participation, utility-based quality of life, balance confidence, technology self-efficacy, falls and service utilisation. Linear models will assess the effect of group allocation for each continuously scored outcome measure with baseline scores entered as a covariate. Fall rates between groups will be compared using negative binomial regression. Primary analyses will be preplanned, conducted while masked to group allocation and use an

  2. The Songbird Neurogenomics (SoNG Initiative: Community-based tools and strategies for study of brain gene function and evolution

    Directory of Open Access Journals (Sweden)

    Lewin Harris A

    2008-03-01

    Full Text Available Abstract Background Songbirds hold great promise for biomedical, environmental and evolutionary research. A complete draft sequence of the zebra finch genome is imminent, yet a need remains for application of genomic resources within a research community traditionally focused on ethology and neurobiological methods. In response, we developed a core set of genomic tools and a novel collaborative strategy to probe gene expression in diverse songbird species and natural contexts. Results We end-sequenced cDNAs from zebra finch brain and incorporated additional sequences from community sources into a database of 86,784 high quality reads. These assembled into 31,658 non-redundant contigs and singletons, which we annotated via BLAST search of chicken and human databases. The results are publicly available in the ESTIMA:Songbird database. We produced a spotted cDNA microarray with 20,160 addresses representing 17,214 non-redundant products of an estimated 11,500–15,000 genes, validating it by analysis of immediate-early gene (zenk gene activation following song exposure and by demonstrating effective cross hybridization to genomic DNAs of other songbird species in the Passerida Parvorder. Our assembly was also used in the design of the "Lund-zfa" Affymetrix array representing ~22,000 non-redundant sequences. When the two arrays were hybridized to cDNAs from the same set of male and female zebra finch brain samples, both arrays detected a common set of regulated transcripts with a Pearson correlation coefficient of 0.895. To stimulate use of these resources by the songbird research community and to maintain consistent technical standards, we devised a "Community Collaboration" mechanism whereby individual birdsong researchers develop experiments and provide tissues, but a single individual in the community is responsible for all RNA extractions, labelling and microarray hybridizations. Conclusion Immediately, these results set the foundation for a

  3. 河南省高校研究生精神信仰状况%The Present Situation of Spiritual Belief amo ng Postgraduates in Henan

    Institute of Scientific and Technical Information of China (English)

    张尚书; 宋春花; 孙利谦; 王鹏

    2014-01-01

    ,genders,professions,SPSS17.0 software was adopted.And repeated ANOVA(analysis of variance) and multivariate ANOVA statistical methods were used for analysis .Results Social belief ranked the first ,then pragmatistic belief,and the supernatural belief was the last;The rank(from high to low)of the nine dimensions on spiritual belief was racialism,life worship,nationalism,familism,political belief,family worship,religion belief,money worship and supernatural belief [F(8,1015) =717.28,P<0.001].Boys had higher scores than girls in family worship and money worship ,however,girls had higher scores in life wor-ship,racialism and nationalism .Grades 1 students had higher than grades 2 students in social belief and political belief ,however,grades 1 students had the lowest scores in money worship .Grades 2 students had the lowest scores in racialism .④Arts students had higher scores than medical students in political belief .Conclusion In the first level classification ,social belief rankes the first ,then pragmatistic belief and the supernatural belief.In the second level classification,racialism,life worship,and nationalism ranke top;religion belief,money worship,and supernatural belief ranke after the three ones (P<0.001).The above results are almost consistent with previous studies . There are significant differences in spiritual belief among different sex ,grades and majors of postgraduates .

  4. Rahvapärane viiulimäng 20.sajandi esimesel poolel Tori ja Vändra viiuldajate näitel

    Directory of Open Access Journals (Sweden)

    Krista Sildoja

    2011-12-01

    Tori fiddlers. Imitating bagpipe is definitely one of the earliest of way ofplaying in terms of age in our old fiddle music. The other way of playing in duo performancesis simple polyphony based on functional harmony.Fiddlers made the melodies of Estonian type of waltzes and polkas more interestingand richer using polyphonic fiddle playing technique and embellishments of melodynotes. They used embellishments (mordents and upbeats when they “felt like it”.Such means make a piece of music airy and general impression of the playing technique rather masterly.The structures of the analysed fiddle pieces are simpler and also of a rather surprising (and random construction as compared to composed classical music. This is what makes the music under survey so exciting. Each piece offers something new and unexpected – prevalently the predictable and expected systematic thinking does not exist. In case of Estonian type of waltzes as well as polkas the most important idea is that the piece of music had a continuous metrum to support dancers in their dance. As a result of form analysis it appeared that different playthroughs of Estonian type of waltzes are freer in terms of their construction, or more improvised, polkas on the other hand are more stable. The dominant factor in the development of playthroughs proved to be the number of members in the performance. If it is a solo performance, the variations in different levels of form are more frequent than in case of a duo performance. It is also understandable as the co-play of at least two people requires previous agreement in approach to form.The playing style in traditional music, as a form of music learnt by ear, is quite individual in each player. At the same time it can be observed that the playing styles of two players living close to each other generally do not differ significantly. The same can be seen in the analysis of the fiddle players’ playing styles in the present research. The playing styles of fiddle

  5. Quantifying the value of investing in distributed natural gas and renewable electricity systems as complements: Applications of discounted cash flow and real options analysis with stochastic inputs

    Energy Technology Data Exchange (ETDEWEB)

    Pless, Jacquelyn; Arent, Douglas J.; Logan, Jeffrey; Cochran, Jaquelin; Zinaman, Owen

    2016-10-01

    One energy policy objective in the United States is to promote the adoption of technologies that provide consumers with stable, secure, and clean energy. Recent work provides anecdotal evidence of natural gas (NG) and renewable electricity (RE) synergies in the power sector, however few studies quantify the value of investing in NG and RE systems together as complements. This paper uses discounted cash flow analysis and real options analysis to value hybrid NG-RE systems in distributed applications, focusing on residential and commercial projects assumed to be located in the states of New York and Texas. Technology performance and operational risk profiles are modeled at the hourly level to capture variable RE output and NG prices are modeled stochastically as geometric Ornstein-Uhlenbeck (OU) stochastic processes to capture NG price uncertainty. The findings consistently suggest that NG-RE hybrid distributed systems are more favorable investments in the applications studied relative to their single-technology alternatives when incentives for renewables are available. In some cases, NG-only systems are the favorable investments. Understanding the value of investing in NG-RE hybrid systems provides insights into one avenue towards reducing greenhouse gas emissions, given the important role of NG and RE in the power sector.

  6. Ideais e realidade: uma aula reservada para crianças autistas bilíngües = Ideals and reality: a self-contained class for bilingual autistic children

    Directory of Open Access Journals (Sweden)

    Ebsworth, Miriam Eisenstein

    2009-01-01

    Full Text Available Este estudo qualitativo abordou as experiências de crianças bilíngües, selecionadas com desordem do espectro autista em uma sala de aula de Educação Infantil Especial Bilíngüe. Os dados incluíram observação ao vivo, gravações em vídeo e entrevistas com os funcionários e pais. Apesar da dedicação dos professores, dos funcionários e da família, os desafios incluíram limitações lingüísticas de clínicos gerais, uso inconsistente de espanhol e/ou inglês, falta de compreensão dos pais sobre a natureza do autismo e demanda irreal de funcionários. O ambiente da barulhenta sala de aula, às vezes refletia aprendizagem, que freqüentemente significava transtorno. A preparação profissional de professores também era inadequada

  7. ng ja maagia / Doris Kareva

    Index Scriptorium Estoniae

    Kareva, Doris, 1958-

    2001-01-01

    20. nov. 2001 arutati Tartu Kirjanike Maja kriitikaseminaril Mehis Heinsaare "Härra Pauli kroonikaid" (2001). Ka Valdo Valperi näitusest Illegaardi galeriis "Eesti kirjandus enne ja pärast Mehis Heinsaart"

  8. In/compatible Imagi(ni)ng

    DEFF Research Database (Denmark)

    Kristensen, Thomas Bjørnsten

    2012-01-01

    The article thematizes a certain logic of reproduction that follows from a point in art history where the construction of images became a process of assembling or coding discrete signals through systematic methods. A logic which is not concerned with imitation but with creating an interchangeable...

  9. HEALTHY AND SAFETY SWİMMİNG

    OpenAIRE

    Süleyman Ceylan

    2005-01-01

    Swimming is a sport which has own rules, styles, and fields, however, is one of the most performed avocation as amateur and a joke especially at summer months. Although one of the most beneficial sports, swimming can cause a number of several health problems such as infectious diseases, allergic events, or traumas, if it is not done at adequate conditions and eligible style. In this paper, the factors such as preparing to swimming, health and safety features of swimming areas, important healt...

  10. Om sammenhæng i rehabiliteringsprocessen

    DEFF Research Database (Denmark)

    de Vries, Alex

    2003-01-01

    and discussed in relation to rehabilitation. This results in a construction and presentation of a patient participation matrix. Findings: The ICF is a potential theoretical framework for rehabilitation, which also can encompass the major characteristics of the concept of democratic health education....... These are patient participation and action competence. The patient participation matrix could be a useful tool to emphasize patient participation during the rehabilitation process. Conclusion: The shape of the rehabilitation process will be more visible by using the ICF as a theoretical framework and the patient...

  11. Sammenhæng i ungdomsuddannelserne

    DEFF Research Database (Denmark)

    Laursen, Erik; Rasmussen, Palle

    2009-01-01

    For efterhånden flere år siden formulerede regeringen en målsætning om, at 95 pct. af alle unge skal gennemføre en ungdomsuddannelse. I dag er der ikke meget der tyder på, at vi nærmer os målet. Tværtimod. I de sidste 15 år har andelen der gennemfører en ungdomsuddannelse ligget omkring 80 pct., ...

  12. Renessanslik vetemäng / Tiina Tammet

    Index Scriptorium Estoniae

    Tammet, Tiina, 1971-

    2009-01-01

    Rooma lähedal Tivoli linnakeses asuvast Itaalia 16. sajandi renessansiaegse palee ja aiakunsti pärlist Villa d'Este, mis on UNESCO maailmapärandi nimekirjas. Pargi ja palee arhitektid Pirro Ligorio ja Alberto Galvani, purskkaevude autoriks skulptor Gianlorenzo Bernini

  13. ng õpetajate palganumbritega / Olav Aarna

    Index Scriptorium Estoniae

    Aarna, Olav, 1942-

    2005-01-01

    Ilmunud ka: Vooremaa 5. nov., lk. 2, Harjumaa 8. nov., lk. 2, Hiiu Leht 8. nov., lk. 2, Virumaa Teataja 8. nov., lk. 7, Lääne Elu 8. nov., lk. 2, Võrumaa Teataja 8. nov. lk. 2, Põhjarannik Severnoje Poberezhje 11. nov. lk. 2, Valgamaalane 12. nov. lk. 2, Pärnu Postimees 16. nov. lk. 15, Sakala 29. nov. lk. 2. Riigikogu kultuurikomisjoni esimees süüdistab haridusminister Mailis Repsi segaduse tekitamises õpetajate palgapoliitikas

  14. PCR Reverse Hybridization Membrane Applications in Screening of CT,UU,NG Three Kinds of STD%PCR反向膜杂交法在CT、UU、NG三种性病筛查中的应用价值

    Institute of Scientific and Technical Information of China (English)

    王涛; 王鹏; 董丽

    2014-01-01

    Objective:To investigate the value of PCR reverse hybridization membrane application in screening of CT,UU,NG three kinds of STD. Method:1486 suspected patients with genitourinary tract infections were detected by PCR reverse hybridization membrane and fluorescence quantitative PCR(FQ-PCR),and test results were compared with culture results. Result:FQ-PCR detection of CT,UU,NG was higher than culture(P<0.01). PCR reverse hybridization membrane,culture and FQ-PCR positive rate difference was not statistically significant. PCR reverse hybridization membrane,FQ-PCR detection sensitivity difference was not statistically significant,but the film PCR reverse hybridization assay specificity was higher than FQ-PCR(P<0.01). Conclusion:PCR reverse hybridization in screening of CT,UU,NG,is accurate,rapid,and specific is higher than FQ-PCR,worthy of promotion apply.%目的:探讨PCR反向膜杂交法在CT、UU、NG三种性病筛查中的应用价值。方法:对1486例疑为泌尿生殖道感染患者分别采用PCR反向膜杂交法及荧光定量PCR(FQ-PCR)检测,并将检测结果与培养结果比较。结果:FQ-PCR检测CT、UU、NG阳性率高于培养法,比较差异有统计学意义(P<0.01);PCR反向膜杂交法与培养法及FQ-PCR检测阳性率比较差异均无统计学意义;PCR反向膜杂交法、FQ-PCR检测敏感性比较差异无统计学意义,但PCR反向膜杂交法检测特异性高于FQ-PCR,比较差异有统计学意义(P<0.01)。结论:PCR反向膜杂交法筛查CT、UU、NG准确,快速,且筛查特异性高于FQ-PCR,值得临床推广适用。

  15. Neisseria gonorrhoeae antimicrobial susceptibility in Barcelona: penA, ponA, mtrR, and porB mutations and NG-MAST sequence types associated with decreased susceptibility to cephalosporins.

    Science.gov (United States)

    Serra-Pladevall, J; Barberá, M J; Rodriguez, S; Bartolomé-Comas, R; Roig, G; Juvé, R; Andreu, A

    2016-09-01

    The aims of this study were to determine the antimicrobial susceptibility of Neisseria gonorrhoeae (NG) in our area, to analyze the molecular mechanisms involved in cephalosporins resistance, and to undertake molecular typing of our NG strains. Antimicrobial susceptibility was determined using the Etest. The genes penA, mtrR, penB, and ponA were studied. Molecular typing was performed by N. gonorrhoeae multiantigen sequence typing. Of 329 strains analyzed in 2013, none showed high-level cephalosporin resistance, but 8.2 % had resistance to cefixime [minimum inhibitory concentration (MIC) > 0.125 μg/mL] and 0.6 % to ceftriaxone (MIC > 0.125 μg/mL). Azithromycin resistance was documented in 4.3 % and ciprofloxacin resistance in 49.2 %. Among 48 strains with an MIC ≥ 0.125 μg/mL to cefixime, 58.3 % showed the penA mosaic pattern XXXIV, 98 % a Leu → Pro substitution at position 421 of the ponA gene, 100 % amino acid changes at positions 101 and 102 of the PorB1b porin, and 87.5 % of strains an adenine deletion in the promoter region of the MtrC-D-E efflux pump. A significant difference between strains with and without decreased cephalosporin susceptibility (MIC ≥ 0.125 μg/mL) was observed for these four genes. Of the 48 strains with an MIC ≥ 0.125 μg/mL to cefixime, 43.8 % belonged to the genogroup G1407 and 27.1 % belonged to the genogroup G2400. A significant association of G1407 with decreased susceptibility (MIC ≥ 0.125 μg/mL) and G2992 with susceptibility was found, and also between G1407 and mosaic pattern XXXIV and between G2400 and A501T substitution in penA. The NG resistance rate in our area is higher than the median of Europe. We have detected the emergence of G2400, which may be a source of antimicrobial resistance. PMID:27255221

  16. NG含量对改性单基发射药燃烧性能的影响%Effect of NG Content on the Burning Performance of Modified Single-base Gun Propellant

    Institute of Scientific and Technical Information of China (English)

    李强; 闫光虎; 张玉成; 严文荣; 张江波; 赵晓梅; 刘强; 杜江媛

    2012-01-01

    The modified single-base gun propellant samples were prepared based on 5/7 single-base gun propellantwith high N content by dipping NG, then desensitizer NA polyester. In the sample preparing process the content ofNA was kept invariable and the mass content of NG was 5% ,10% and 15% , respectively. Three samples, |RN-a,RN-b and RN-c, were prepared. The effect of NG content on the burning performance of modified single-base gunpropellant was studied based on the closed bomb test and interior ballistic test with 30mm gun. The results showthat the burning progressivity of all the three samples is better than that of original 5/7 single-base gun propellant.Under equivalent maximum pressure, muzzle velocity of the RN-a, RN-b and RN-c propellant charges is increasedby 36. 9, 91. 7 and 57. 4m/s respectively compared to the original 5/7 single-base gun propellant. The performanceof modified single-base gun propellant can be optimized by adjusting the content of NG, so that the muzzle velocitycan be greatly increased.%以高氮量5/7单基发射药为基体药粒,通过先浸渍吸收NG、后吸收聚酯钝感剂NA的两步工艺制备出改性单基药,在制备过程中,NA含量恒定,浸渍NG的质量分数为5%、10%和15%,制备出3种NG含量的改性单基药样品RN-a、RN-b和RN-c.采用密闭爆发器试验和30 mm弹道炮内弹道试验,研究了NG含量对改性单基药燃烧性能的影响.结果表明,所制备的3种改性单基药样品的燃烧渐增性均优于基体药;在保持最大膛压不增加的情况下,弹丸初速较5/7单基药装药分别提高36.9、91.7和57.4m/s.在制备工艺过程中,通过调节NG的含量,能够改善改性单基药的燃烧性能,实现大幅度提高炮口初速的目的.

  17. Organização e qualidade da assistência à saúde dos índios Kaingáng do Rio Grande do Sul, Brasil

    Directory of Open Access Journals (Sweden)

    Yara Hahr Marques Hökerberg

    2001-03-01

    Full Text Available Este estudo pretende avaliar o atendimento à saúde prestado aos índios Kaingáng do Rio Grande do Sul. Os óbitos evitáveis por assistência básica de saúde dos indígenas, ocorridos entre 1985 e 1995, foram comparados aos óbitos do Estado do Rio Grande do Sul. Os dados secundários relativos aos serviços de saúde foram complementados por entrevistas realizadas em campo com lideranças indígenas e com funcionários das instituições envolvidas na assistência à saúde do índio. Foi utilizado o Sistema de Informações Geográficas, visando relacionar a distribuição dos óbitos ao grau de acesso aos serviços de saúde. As áreas indígenas Kaingáng estão ligadas por estradas pavimentadas a municípios que contam com, pelo menos, um centro de saúde, ou ainda, hospitais de pequeno porte. O segundo nível de atenção situa-se em Palmeiras das Missões e Frederico Westphallen e o terceiro nível, em Passo Fundo. O que diferencia estas áreas indígenas do restante do Estado do Rio Grande do Sul é a quantidade de óbitos evitáveis pela assistência básica de saúde, tais como as causas mal definidas, a desnutrição, a tuberculose e o câncer cérvico-uterino.

  18. The fluidity of Chinese Americans'ethnic and sexual identity in Fae Myenne Ng's Bone%伍慧明《骨》中美籍华人种族及性别身份流变

    Institute of Scientific and Technical Information of China (English)

    周燕萍

    2014-01-01

    Identity has been a focus in post-colonial studies .Chinese American writer Fae Myenne Ng's debut Bone excavates Chinese Americans'ethnic and sexual identification under feminist , anti-colonial and anti-racist move-ments.Using Stuart Hall's conception of fluid identity as the theoretical foundation ,and Bone as text,this thesis in-tends to explore Fae Myenne Ng'effort in deconstructing ,constructing and reconstructing Chinese Americans ethnic and sexual identities based on different social ,historical and political backgrounds ,which well illustrates post-colo-nial identity is not in a static state ,but in a dynamic state with constant changing transformations .%身份问题一直以来是后殖民研究中的焦点。美籍华裔作家伍慧明的首部作品《骨》因揭示了民权运动、女权运动背景下的美籍华裔寻找身份的苦痛历程而备受关注。本文以斯图亚特·霍尔的身份流变论为理论,通过对《骨》的文本分析,探寻不同社会、历史及政治背景下美籍华人民族及性别身份的解构、建构与重建,例证后殖民身份不是静态的,而是处于不断流变的过程当中。

  19. Organização e qualidade da assistência à saúde dos índios Kaingáng do Rio Grande do Sul, Brasil

    Directory of Open Access Journals (Sweden)

    Hökerberg Yara Hahr Marques

    2001-01-01

    Full Text Available Este estudo pretende avaliar o atendimento à saúde prestado aos índios Kaingáng do Rio Grande do Sul. Os óbitos evitáveis por assistência básica de saúde dos indígenas, ocorridos entre 1985 e 1995, foram comparados aos óbitos do Estado do Rio Grande do Sul. Os dados secundários relativos aos serviços de saúde foram complementados por entrevistas realizadas em campo com lideranças indígenas e com funcionários das instituições envolvidas na assistência à saúde do índio. Foi utilizado o Sistema de Informações Geográficas, visando relacionar a distribuição dos óbitos ao grau de acesso aos serviços de saúde. As áreas indígenas Kaingáng estão ligadas por estradas pavimentadas a municípios que contam com, pelo menos, um centro de saúde, ou ainda, hospitais de pequeno porte. O segundo nível de atenção situa-se em Palmeiras das Missões e Frederico Westphallen e o terceiro nível, em Passo Fundo. O que diferencia estas áreas indígenas do restante do Estado do Rio Grande do Sul é a quantidade de óbitos evitáveis pela assistência básica de saúde, tais como as causas mal definidas, a desnutrição, a tuberculose e o câncer cérvico-uterino.

  20. Th? Imp?ct of F?n?nc??l R?port?ng of Sm?ll ?nd M?d?um S?z?d Ent?rpr?s?s ?n Econom?c D?v?lopm?nt of B?lk?n Countr??s

    OpenAIRE

    Artor Nuhiu; Arber Hoti; Lulzim Krasniqi

    2012-01-01

    Th? ?mport?nc? of ?nt?rn?l ?nd ?xt?rn?l ?ccount?ng ?s ? sourc? of ?nform?t?on for own?rs ?nd m?n?g?rs of sm?ll ?nt?rpr?s?s ?nd th??r d?ff?r?nt st?k?hold?rs ?s st??d?ly grow?ng. It ?s of cruc??l ?mport?nc? th?t th? ?ccount?ng syst?ms ?ppl??d by sm?ll ?nt?rpr?s?s m??t th??r ?ctu?l n??ds, prov?d?ng n?c?ss?ry ?nform?t?on y?t ?vo?d?ng unjust?f??d ?dm?n?str?t?v? burd?n. It ?s r?cogn?s?d th?t ?ppropr??t? ?ccount?ng ?nform?t?on ?s ?mport?nt for ? succ?ssful m?n?g?m?nt of ? bus?n?ss wh?th?r ?t ?s l?rg...

  1. Performance Analysis of Dual-Hop Relaying System over Mixture Gamma Fadi ng Channel s%两跳中继系统在混合Gamma衰落信道下的性能分析

    Institute of Scientific and Technical Information of China (English)

    程卫军

    2014-01-01

    The end-to-end performance of a dual-hop amplify-and-forward relaying system is investigated over composite multipath-lognormal fading channels by using Mixture Gamma (MG )channel model .Novel exact closed-form expressions for the cu-mulative distribution function and the moment-generation function of the end-to-end signal-to-noise ratio are derived .Moreover ,the average symbol error rate ,outage probability and the diversity order are found based on the above new expressions ,respectively . These expressions are more simple and accuracy than the ones obtained by using Generalized-K(KG)channel model .Finally ,numer-ical and simulation results are shown to verify the accuracy of the analytical results .%基于混合Gamma信道模型研究了两跳中继系统在联合多径阴影衰落环境下的性能。推导了两跳中继系统端到端信噪比的累积分布函数和矩生函数的精确闭式解,并基于这些闭式解分析了两跳中继系统的误码率、中断率和分集增益等性能。与广义K衰落模型下的性能进行了比较,运用混合Gamma衰落模型可获得简单精确的结果。最后数值和仿真分析验证了理论研究的正确性。

  2. (R-(+-α-Lipoic acid protected NG108-15 cells against H2O2-induced cell death through PI3K-Akt/GSK-3β pathway and suppression of NF-κβ-cytokines

    Directory of Open Access Journals (Sweden)

    Kamarudin MNA

    2014-10-01

    Full Text Available Muhamad Noor Alfarizal Kamarudin, Nur Afiqah Mohd Raflee, Sharifah Salwa Syed Hussein, Jia Ye Lo, Hadi Supriady, Habsah Abdul KadirInstitute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, MalaysiaAbstract: Alpha-lipoic acid, a potent antioxidant with multifarious pharmacological benefits has been reported to be neuroprotective in several neuronal models and used to treat neurological disorders such as Alzheimer’s disease. Nonetheless, conclusive mechanisms of alpha-lipoic acid for its protective effects particularly in NG108-15 cells have never been investigated. In this study, the intricate neuroprotective molecular mechanisms by (R-(+-alpha-lipoic acid (R-LA against H2O2-induced cell death in an in vitro model of neurodegeneration were elucidated. Pretreatment with R-LA (2 hours significantly increased NG108-15 cell viability as compared to H2O2-treated cells and mitigated the induction of apoptosis as evidenced by Hoechst 33342/propidium iodide staining. R-LA (12.5–50 µM aggrandized the reduced glutathione over glutathione disulfide ratio followed by a reduction in the intracellular reactive oxygen species level and an increase in mitochondrial membrane potential following H2O2 exposure. Moreover, pretreatment with R-LA stimulated the activation of PI3K-Akt through mTORC1 and mTORC2 components (mTOR, rictor and raptor and production of antiinflammatory cytokine, IL-10 which led to the inactivation of glycogen synthase kinase-3β (GSK-3β and reduction of both Bax/Bcl2 and Bax/Bcl-xL ratios, accompanied by inhibition of the cleaved caspase-3. Additionally, this observation was preceded by the suppression of NF-κβ p65 translocation and production of proinflammatory cytokines (IL-6 and TNF-α. The current findings accentuate new mechanistic insight of R-LA against apoptogenic and brain inflammatory factors in a neuronal model. These results further advocate the therapeutic potential of R-LA for

  3. Ciclo biológico de Paraibatrema inesperata n.g., n.sp. (Trematoda, Paramphistomidae, a partir de metacercárias desenvolvidas em Biomphalaria tenagophila (D'Orbigny, 1835 (Mollusca, Planorbidae

    Directory of Open Access Journals (Sweden)

    Marlene Tiduko Ueta

    1981-03-01

    Full Text Available Em exemplares de B. tenagophila, capturados em ambiente natural, foram encontradas paranfistomocercárias de precoce encistamento. Procedeu-se à infecção, per os, de animais de biotério; em camundongos e ratos, verificou-se o desenvolviemnto de trematóide paranfistomídeo desconhecido e que, por suas características, não se enquadra nos g~eneros conhecidos de Paramphistomidae de mamíferos. Para o parasito em apreço é proposta a denominação Paraibatema inesperata n.g, n.sp.. A partir de miracídios, oriundos de ovos eliminados pelos roedores em experiência, procurou-se infectar B. tenagophila e B. glabrata, criadas no moluscário. Verificou-se ser ativa a penetração dos miracídios no tecido do molusco, sendo freqüente a formação de tumorações nas antenas. A evolução experimental no hospedeiro intermediário apresenta-se lenta; ao fim de 40 dias de pós-infecção, não se observou a formação de esporocistos, rédias e cercárias.The new genus and new species of paramphistomid trematode, Paraibatrema inesperata n.g, n.sp., are proposed. The parasite was originally found as a quickly encysting paramphistome cercaria from specimens of the planorbid snail Biomphalaria tenagophila caught in natural habitats. Experimental infection resulted from intubation of metacercariae into the stomach of white mice and rats, which passed eggs in the feces 20 days later. Specimens of B. tenagophila and B. glabrata have been exposed to miracidia from eggs passed by mice. Penetration of the molluscan tegument was observed, but it seems that the larval development of the parasite is slow, inasmuch as no rediae and cercariae have been obtained as yet.

  4. Impact of Comorbidity, Race, and Marital Status in Men Referred for Prostate Biopsy with PSA >20 ng/mL: A Pilot Study in High-Risk Patients.

    Science.gov (United States)

    Klaassen, Zachary; Muller, Roberto; Li, Qiang; Tatem, Alexander J; King, Sherita A; Freedland, Stephen J; Madi, Rabii; Terris, Martha K; Moses, Kelvin A

    2014-01-01

    Objective. To assess the impact of comorbidity, race, and marital status on overall survival (OS) among men presenting for prostate biopsy with PSA >20 ng/mL. Methods. Data were reviewed from 2000 to 2012 and 78 patients were included in the cohort. We analyzed predictors of OS using a Cox proportional hazards model and the association between Charlson Comorbidity Index (CCI) score and PCa diagnosis or high-grade cancer using logistic regression and multinomial regression models, respectively. Results. The median age of patients was 62.5 (IQR 57-73) years. Median CCI was 3 (IQR 2-4), 69% of patients were African American men, 56% of patients were married, and 85% of patients had a positive biopsy. CCI (HR 1.52, 95% CI 1.19, 1.94), PSA (HR 1.62, 95% CI 1.09, 2.42), and Gleason sum (HR 2.04, 95% CI 1.17, 3.56) were associated with OS. CCI was associated with Gleason sum 7 (OR 4.06, 95% CI 1.04, 15.89) and Gleason sum 8-10 (OR 4.52, 95% CI 1.16, 17.54) PCa. Conclusions. CCI is an independent predictor of high-grade disease and worse OS among men with PCa. Race and marital status were not significantly associated with survival in this cohort. Patient comorbidity is an important component of determining the optimal approach to management of prostate cancer.

  5. Frammistöðukvíði í tengslum við söng og tónlistarflutning : helstu ráð og meðferðarform

    OpenAIRE

    Elísabet Ólafsdóttir 1977

    2016-01-01

    Frammistöðukvíða þekkja líklega flestir sem hafa talað, sungið eða flutt tónlist fyrir framan áheyrendur. Tilgangur þessarar ritgerðar er að taka saman rannsóknir um helstu ráð og meðferðarform við frammistöðukvíða og meta aðferðafræði þeirra og niðurstöður á gagnrýninn hátt í þeirri von að sú samantekt megi nýtast í tónlistar- og söngkennslu á Íslandi. Við leit í rafrænum gagnagrunnum fundust alls 13 ritrýndar rannsóknargreinar varðandi frammistöðukvíða sem tengdust tónlist og/eða söng með b...

  6. The Politics of Frontier Mining: Local Chieftains, Chinese Miners, and Upland Society in the Nông Văn Vân Uprising in the Sino-Vietnamese Border Area, 1833–1835

    Directory of Open Access Journals (Sweden)

    Vũ Đường Luân

    2014-06-01

    Full Text Available This article, part of a longer study of the history of mining in Vietnam, argues that the Nông Văn Vân uprising (1833–1835 in the northern uplands of Vietnam brings into relief the importance of mining in the Vietnamese economy of the nineteenth century. It also highlights the consequences of Emperor Minh Mệnh’s dual agenda of extracting more tax revenues from mining operations and expanding the reach of the state by replacing hereditary tribal chieftains with imperial bureaucrats. While the uprising was quelled, the imperial agenda could not be fully realized in the face of local opposition and declining revenue from mining. The uprising reflected the multiethnic nature of border society, composed as it was of Vietnamese, local minority populations, and a significant number of Chinese mine owners, workers, and providers of goods and services to the mining towns. In ordinary times, the border was regularly flouted as kinship relations, trading networks, and ethnic affinity transcended allegiance to either the Qing or the Nguyễn in this borderland. Although the uprising was formally contained within the Vietnamese territory, rebels were able to seek refuge and recruit new adherents in China. And while the Nguyễn court was eventually able to subdue the rebels, its centralizing policies and attempts at extracting more revenues from mining were ultimately unsuccessful in the face of reemerging local and transborder forces.

  7. Impact of Comorbidity, Race, and Marital Status in Men Referred for Prostate Biopsy with PSA >20 ng/mL: A Pilot Study in High-Risk Patients.

    Science.gov (United States)

    Klaassen, Zachary; Muller, Roberto; Li, Qiang; Tatem, Alexander J; King, Sherita A; Freedland, Stephen J; Madi, Rabii; Terris, Martha K; Moses, Kelvin A

    2014-01-01

    Objective. To assess the impact of comorbidity, race, and marital status on overall survival (OS) among men presenting for prostate biopsy with PSA >20 ng/mL. Methods. Data were reviewed from 2000 to 2012 and 78 patients were included in the cohort. We analyzed predictors of OS using a Cox proportional hazards model and the association between Charlson Comorbidity Index (CCI) score and PCa diagnosis or high-grade cancer using logistic regression and multinomial regression models, respectively. Results. The median age of patients was 62.5 (IQR 57-73) years. Median CCI was 3 (IQR 2-4), 69% of patients were African American men, 56% of patients were married, and 85% of patients had a positive biopsy. CCI (HR 1.52, 95% CI 1.19, 1.94), PSA (HR 1.62, 95% CI 1.09, 2.42), and Gleason sum (HR 2.04, 95% CI 1.17, 3.56) were associated with OS. CCI was associated with Gleason sum 7 (OR 4.06, 95% CI 1.04, 15.89) and Gleason sum 8-10 (OR 4.52, 95% CI 1.16, 17.54) PCa. Conclusions. CCI is an independent predictor of high-grade disease and worse OS among men with PCa. Race and marital status were not significantly associated with survival in this cohort. Patient comorbidity is an important component of determining the optimal approach to management of prostate cancer. PMID:27355056

  8. On the Lighting Failure Caused by EMDP Overheating of Boeing 737NG Airplane%波音737NG飞机EMDP过热灯亮故障浅析

    Institute of Scientific and Technical Information of China (English)

    魏小刚; 张爱学

    2014-01-01

    波音737NG飞机在运行中,液压系统故障出现频率较高,对飞机的正常运行产生了很大的障碍,其中EMDP(电子马达驱动泵)过热问题也造成了不少的不正常航班事件,对航班的运营产生了很大的障碍,本文主要对EMDP过热灯亮故障进行浅析。%The occurrence frequency of hydranlic problems of Boeing 737NG Airplane is high in the running. It has great obstacles of the airplane's normal operation. The overheating of EMDP (Electronic Motor Drive Pump) causes many abnormal flight events and makes great obstacles for the operation and production of the flight. This paper analyzes the lighting failure caused by EMDP overheating.

  9. Pre-combustion CO2 capture: Analysis of integrated reforming combined cycle

    OpenAIRE

    Nord, Lars Olof

    2010-01-01

    This thesis presents processes for reducing CO2 emissions from natural gas (NG) power plants, which could help attenuate the rise in atmospheric temperature. Objectives for the thesis work were process design and integration of NG pre-combustion CO2 capture plants, and evalution, through process simulation, of the concepts. An important aspect of the evaluation was investigation of plant flexibility, specifically off-design analysis. Contributions comprised detailed heat recovery steam genera...

  10. Pre-combustion CO2 capture: Analysis of integrated reforming combined cycle

    OpenAIRE

    Nord, Lars Olof

    2010-01-01

    This thesis presents processes for reducing CO2 emissions from natural gas (NG) power plants, which could help attenuate the rise in atmospheric temperature. Objectives for the thesis work were process design and integration of NG pre-combustion CO2 capture plants, and evalution, through process simulation, of the concepts. An important aspect of the evaluation was investigation of plant flexibility, specifically off-design analysis. Contributions comprised detailed heat recovery steam genera...

  11. Analysis

    CERN Document Server

    Maurin, Krzysztof

    1980-01-01

    The extraordinarily rapid advances made in mathematics since World War II have resulted in analysis becoming an enormous organism spread­ ing in all directions. Gone for good surely are the days of the great French "courses of analysis" which embodied the whole of the "ana­ lytical" knowledge of the times in three volumes-as the classical work of Camille Jordan. Perhaps that is why present-day textbooks of anal­ ysis are disproportionately modest relative to the present state of the art. More: they have "retreated" to the state before Jordan and Goursat. In recent years the scene has been changing rapidly: Jean Dieudon­ ne is offering us his monumentel Elements d'Analyse (10 volumes) written in the spirit of the great French Course d'Analyse. To the best of my knowledge, the present book is the only one of its size: starting from scratch-from rational numbers, to be precise-it goes on to the theory of distributions, direct integrals, analysis on com­ plex manifolds, Kahler manifolds, the theory of sheave...

  12. Analysis

    Science.gov (United States)

    Abdelazeem, Maha; El-Sawy, El-Sawy K.; Gobashy, Mohamed M.

    2013-06-01

    Ar Rika fault zone constitutes one of the two major parts of the NW-SE Najd fault system (NFS), which is one of the most prominent structural features located in the east of the center of the Arabian Shield, Saudi Arabia. By using Enhancement Thematic Mapper data (ETM+) and Principle Component Analysis (PCA), surface geological characteristics, distribution of rock types, and the different trends of linear features and faults are determined in the study area. First and second order magnetic gradients of the geomagnetic field at the North East of Wadi Ar Rika have been calculated in the frequency domain to map both surface and subsurface lineaments and faults. Lineaments as deduced from previous studies, suggest an extension of the NFS beneath the cover rocks in the study area. In the present study, integration of magnetic gradients and remote sensing analysis that resulted in different valuable derivative maps confirm the subsurface extension of some of the surface features. The 3D Euler deconvolution, the total gradient, and the tilt angle maps have been utilized to determine accurately the distribution of shear zones, the tectonic implications, and the internal structures of the terranes in the Ar Rika quadrangle in three dimensions.

  13. Pagsasatinig ng Pagkabata: Ang Varayti ng Wika sa mga Premyadong Kuwentong Pambata

    Directory of Open Access Journals (Sweden)

    Wennielyn F. Fajilan

    2014-12-01

    Full Text Available Writing for children is a way of cultivating their minds. As a special kind of communication, children’s literature entails the use of a language that caters to the needs and skills of the child reader. The study aims to analyse the variety of language used for children in the narratives of eight prize winning children’s stories—awarded the Palanca Award and Philippine Board of Books for Young People (PBBY prize from 1995-2009—with themes on motherhood or fatherhood. The study found that concepts of motherhood such as that of the traditional homemaker, the OFW, the single mother, and lesbian mothers; concepts of fatherhood like the OFW father, environmentalist and revolutionary parents characterize the selected stories. Through an examination of the dialogues, actions, and descriptions written for the child reader, the paper illustrates how narratives of the self, orality, and intelligence characterize the child protagonist. Hence, an examination of the contemporary children’s stories’ language variety is also an investigation of the prevailing representation of children in Philippine language, literature, and society.

  14. Ang Dumaracol ng mga Kalamianen Tagbanua ng Hilagang Palawan: Isang Panimulang Paglalahad

    Directory of Open Access Journals (Sweden)

    Eulalio R. Guieb III

    2014-12-01

    Full Text Available This study is the first documentation of the long narrative song dumaracol of the Kalamianen Tagbanua of Northern Palawan on the islands bordering the towns of Taytay, El Nido, and Linapacan. The essay is the first in a multi-stage research that collects, records, documents, transcribes, translates into Filipino, and examines the said genre. In the song, Dumaracol is the name of the hero of the Kalamianen Tagbanua. The song narrates the life, love, and struggles of Dumaracol in establishing and maintaining their community against the slave-raiding Moro. The essay discusses the different versions of the song, but highlights that by Blas Juan, one of the contemporary leaders of the Kalamianen Tagbanua of Pical Island in Linapacan. The essay also presents the processes of the research documentation of the song. It identifies and brings to the fore various issues in the preliminary understanding of dumaracol.

  15. Dance Dance Revolution: Usapin ng Laro at Sayaw sa Panahon ng Globalisasyon

    Directory of Open Access Journals (Sweden)

    Jema M. Pamintuan

    2001-06-01

    Full Text Available The Dance Dance Revolution (DDR Machine is becoming a favorite game among the youth and even the not-so-young. While much has been written on the mechanics and popularity of the game, this essay aims to probe into the DDR phenomenon as a parallel tool and response to the changes brought about by technology and the age of globalization. The individual's relationship with the machine, with his audience, and with the larger society will be documented and juxtaposed with the activities of a global village. The cultural implications of the DDR will also be discussed, especially how the game itself revolutionized our traditional concept of game and dance.

  16. Re-imahinasyon ng Ina sa Panitikan ng Kababaihan: Isang Imbestigasyon sa Ideolohiyang Maternal sa Panitikan

    Directory of Open Access Journals (Sweden)

    Rosario Torres-Yu

    2000-06-01

    Full Text Available The narratives generated by my earlier study of 20 women writers from three generations were a rich source of data and insights on many issues on gender and writing, such as motherhood and its representation in literature. It is their ideas on motherhood, which led me to ask how some of them represent motherhood in their works and what these representations say about their positions on the dominant maternal ideology in Philippine culture.Another impetus came from Delia Aguilar's study on Filipino housewives and the politics of gender. This study reveals that " . . . in the private space of the family as the major mechanism for the reproduction of social relations, women are at once validated and oppressed, a process that is powerfully mediated by the maternal ideology of which women are the foremost proponents." This maternal ideology shapes Filipino women's idea of motherhood that sees devotion to children, self-sacrifice, and care giving as the essence of motherhood, allowing them to embrace oppression and subordination. In this context, the interrogation of maternal ideology in Philippine literature is important to the overall feminist movement. Women writers who have freed themselves from the mystifying notions of motherhood may have been inspired to represent women in their works differently.This paper clearly validates such assumption. Writers like Liwayway Arceo, Genoveva Edroza-Matute, Fanny Garcia, Lualhati Bautista, Lilia Quindoza-Santiago, and Luna Sicat-Cleto, as this reading of some of their works reveals, challenge the dominant maternal ideology by re-imagining motherhood. Without wholly rejecting the traditional notion of motherhood (with the exception of Luna Sicat-Cleto who questions the so called "essence" of motherhood, these writers interrogate the idealization of motherhood, question some of the fundamental assumptions of the dominant maternal ideology and represent women as mothers who are nurturing and caring just like the typical image of a Filipino mother, but who are not happy with confinement in the private domestic space. This radical representation of motherhood is, therefore, a welcome development in the present development of feminist discourse in the Philippines.

  17. The Case Method: "I’m not sure ..." Faci ng Barriers to Implementi ng the Method

    Directory of Open Access Journals (Sweden)

    Roberta Dias Campos

    2014-03-01

    extension courses for teachers. It presents the dilemma experienced by Cosmo, a young professor recently hired by the iesg (Institute of Higher Education in Management, who wishes to adopt the case method as a teaching practice within an institutional setting and with distinct and heterogeneous didactic experiences. It also presents the views of different stakeholders in the institution, discussing the challenge to accommodate its teaching strategy with its professionals’ individual and cultural daily practice. The case intends to foster reflection on the barriers and actions required for institutional case method adoption, seen from three of the organization’s levels: the faculty, the student body and the institutional structure as a whole.

  18. Pagsasatinig ng Pagkabata: Ang Varayti ng Wika sa mga Premyadong Kuwentong Pambata

    OpenAIRE

    Wennielyn F. Fajilan

    2014-01-01

    Writing for children is a way of cultivating their minds. As a special kind of communication, children’s literature entails the use of a language that caters to the needs and skills of the child reader. The study aims to analyse the variety of language used for children in the narratives of eight prize winning children’s stories—awarded the Palanca Award and Philippine Board of Books for Young People (PBBY) prize from 1995-2009—with themes on motherhood or fatherhood. The study found that con...

  19. Ang Dalumat ng Panahon at Espasyo sa Mga Traki ng Dulangan Manobo

    Directory of Open Access Journals (Sweden)

    Rosario Cruz-Lucero

    2002-06-01

    Full Text Available This paper introduces the duyuy nga traki “narrative chants” of the Dulangan Manobo, one of the least-documented subtribes of the large Manobo tribe inhabiting most of Mindanao. It attempts an interpretation of their concept of time and space as expressed, implicitly or explicitly, in these traki. The empirical bases for these interpretations are cultural, political and historical data, which are woven into theanalyses. Contemporary data include the tribe’s attempts to assert their Ancestral Domain Claims. Their creation myth, trickster tale, origin stories, etiological legends about natural formations, etc. are interpreted in the light of their economic system and the shifts from one occupational practice to another; historical experiences, such as the coming of outsiders, viz., the Muslims, Americans and homesteaders; and the politics of their location.The narrative chants were recorded in the original Manobo while an interpreter translated these line-by-line into Hiligaynon, which I then translated in Filipino. The narrative chants presented in this paper are the translations into Filipino. This research was conducted in the municipality of Kulaman and the village of Todog, both in the province of Sultan Kudarat. Two weeklong visits were made by this writer to these areas in October 2002 and May 2003, primarily to record the duyuy nga traki performed by Baha Lambay Beliyan, and to interact informally with the Dulangans.

  20. 3c/4e [small sigma, Greek, circumflex]-type long-bonding competes with ω-bonding in noble-gas hydrides HNgY (Ng = He, Ne, Ar, Kr, Xe, Rn; Y = F, Cl, Br, I): a NBO/NRT perspective.

    Science.gov (United States)

    Zhang, Guiqiu; Li, Hong; Weinhold, Frank; Chen, Dezhan

    2016-03-21

    Noble-gas hydrides HNgY are frequently described as a single ionic form (H-Ng)(+)Y(-). We apply natural bond orbital (NBO) and natural resonance theory (NRT) analyses to a series of noble-gas hydrides HNgY (Ng = He, Ne, Ar, Kr, Xe, Rn; Y = F, Cl, Br, I) to gain quantitative insight into the resonance bonding of these hypervalent molecules. We find that each of the studied species should be better represented as a resonance hybrid of three leading resonance structures, namely, H-Ng(+ -):Y (I), H:(- +)Ng-Y (II), and H^Y (III), in which the "ω-bonded" structures I and II arise from the complementary donor-acceptor interactions nY → σ*HNg and nH → σ*NgY, while the "long-bond" ([small sigma, Greek, circumflex]-type) structure III arises from the nNg → [small sigma, Greek, circumflex]*HY/[small sigma, Greek, circumflex]HY interaction. The bonding for all of the studied molecules can be well described in terms of the continuously variable resonance weightings of 3c/4e ω-bonding and [small sigma, Greek, circumflex]-type long-bonding motifs. Furthermore, we find that the calculated bond orders satisfy a generalized form of "conservation of bond order" that incorporates both ω-bonding and long-bonding contributions [viz., (bHNg + bNgY) + bHY = bω-bonding + blong-bonding = 1]. Such "conservation" throughout the title series implies a competitive relationship between ω-bonding and [small sigma, Greek, circumflex]-type long-bonding, whose variations are found to depend in a chemically reasonable manner on the electronegativity of Y and the outer valence-shell character of the central Ng atom. The calculated bond orders are also found to exhibit chemically reasonable correlations with bond lengths, vibrational frequencies, and bond dissociation energies, in accord with Badger's rule and related empirical relationships. Overall, the results provide electronic principles and chemical insight that may prove useful in the rational design of noble-gas hydrides of

  1. INVESTIGATION OF THE METHODS OF COPİNG WİTH STRESS OF PHYSİCAL EDUCATION TEACHERS THAT WORK IN PRİMARY AND SECONDARY EDUCATİON SCHOOL (EXAMPLE OF AYDIN

    Directory of Open Access Journals (Sweden)

    Hüseyin KIRIMOĞLU

    2011-08-01

    Full Text Available Open Journal Systems Journal Help User You are logged in as...hazarserkan •My Journals•My Profile•Log OutLanguage English Türkiye Türkçesi Journal Content Search All Authors Title Abstract Index terms Full Text Browse •By Issue•By Author•By Title•Other JournalsFont Size Make font size smaller Make font size default Make font size larger HomeAboutUser HomeSearchCurrentArchivesAnnouncementsHome > Vol 5, No 2 (2011 > KIRIMOĞLU INVESTIGATION OF THE METHODS OF COPİNG WİTH STRESS OF PHYSİCAL EDUCATION TEACHERS THAT WORK IN PRİMARY AND SECONDARY EDUCATİON SCHOOL (EXAMPLE OF AYDINHüseyin KIRIMOĞLU, Yunus YILDIRIM, Ali TEMİZAbstractThis investigation was performed for discover of whether the methods of coping with stres of physical educatıon teachers that work in Aydın change or not.96 physical education teachers (28 women and 68 men that work in primary and secondary education school in Aydın (country, town, village took part in thıs investigation. For detection of methods of coping with stress; personal information form improved by investigator and ‘ scale of coping with stress ’ improved by original (Özbay, 1993 were used. SPSS 16.0 statistic package programme was used for evaluate of data and find the calculated data. For trial of normality, t test (whose group number is 2 and One – Way ANOVA test (whose group number is more than 2 were used within parametric tests. For variance homology; Tukey HSD test was used within Post Hoc Multiple Comparisions tests. In thıs study ratio of fault was assessed 0.05. When we compared the participants’s methods of coping with stress by some variable factors; statistical significiant differences were found in point of gender; asking for help from others, in point of civil status; taking shelter for religious, in multiply comprision for mission place (country, town, village; taking shelter for religious, active planning, escape-abstraction (biochemical. We can say that there is

  2. The impact of heavy metals from environmental tobacco smoke on indoor air quality as determined by Compton suppression neutron activation analysis.

    Science.gov (United States)

    Landsberger, S; Wu, D

    1995-12-01

    The method of instrumental neutron activation analysis (NAA) has been improved for air filter samples in the determination of low level heavy metals in indoor air. By using the techniques of epithermal neutron irradiation in conjunction with Compton suppression, the detection limits of cadmium, arsenic and antimony measurements have been dramatically reduced to 2 ng for Cd, 0.2 ng for As, and 0.03 ng for Sb. The determination of these heavy metals in particulate material generated from cigarette smoking in indoor environments has been conducted. Other elements, Br, Cl, Na, K, Zn were also found at elevated levels. PMID:8560226

  3. Ruh Adam Romanında Kahramanın Sonsuz Yolculuğu: Yasak Aşk’ın Döngüsü The Endless Journey of Hero in Ruh Adam Novel: Cycle of Forbidden Love

    Directory of Open Access Journals (Sweden)

    Gürhan ÇOPUR

    2012-09-01

    şadığı olumsuzluklar neticesinde içerisine düştüğü bunalım ile başlayan olaylar, onun yaşamının dikey eksende çizilen sonuna doğru başkişiyi kendi iradesi dışında sürükleyecektir. Romanda, başkişi tarafından yaşamın anlamlandırılamayan/yönlendirilemeyen kodları yaşamını çevreler ve onu bir yeniden doğuş sanrısıyla kuşatır. Yeniden doğuşunu klasik bir reenkarnasyon olarak değil simgesel düzlemde önceden hazırlanmış bir döngünün merkezinde yaşayan Selim Pusat, mücadele ettikçe içerisinde kaybolacağı bunalımın derinliklerinde yaşamının en kutsal değerlerinin benliğini sıkıştırmasıyla geçmiş ve bugünün tuğlaları ile örülen bir labirentin duvarları arasında sıkışır. Selim Pusat, içerisinden çıkamadığı bu „labirent‟te yakınlarının şahsında vücut bulan kendilik değerleri ile hesaplaşmasından sembolik anlamda yok oluşuna kadar uzanan çizgide ele alınır.Bu çalışmada, geçmişinin döngüselliğine karşı çaresiz kalan ve yazgısını karşılıksız bir aşkın lanetinde yokluğa karışmakla yaşayan başkişi Selim Pusat‟ın çiğnediği benlik değerleri tarafından yargılanışı ele alınacaktır. Yolculuk, temel anlamı dışında bireyin yaşantısında kader ve kendi adımlarıyla oluşturduğu yaşanmışlıklar bütününün adıdır. Bu yolculukta kurulan bütün aşamalar başkişinin özsel anlamdaki değişimleri, dönüşümleri ve psikolojik durumları bir bütün olarak değerlendirilir. Selim Pusat‟ın yaşamı da arketipsel anlamda yolculuk imgesi üzerine değerlendirildiğinde ortaya bir aşk romanından ötesi çıkar. İzlenecek yolda Joseph Campbell‟in “Kahramanın Sonsuz Yolculuğu” adlı eserinde ele alınan arketipsel dönüşüm aşamaları merkeze alınacaktır.

  4. HPLC-F analysis of melatonin and resveratrol isomers in wine using an SPE procedure.

    Science.gov (United States)

    Mercolini, Laura; Addolorata Saracino, Maria; Bugamelli, Francesca; Ferranti, Anna; Malaguti, Marco; Hrelia, Silvana; Raggi, Maria Augusta

    2008-04-01

    An original analytical method has been developed for the determination of the antioxidants trans-resveratrol (t-RSV) and cis-resveratrol (c-RSV) and of melatonin (MLT) in red and white wine. The method is based on HPLC coupled to fluorescence detection. Separation was obtained by using a RP column (C8, 150 mm x 4.6 mm id, 5 mum) and a mobile phase composed of 79% aqueous phosphate buffer at pH 3.0 and 21% ACN. Fluorescence intensity was monitored at lambda = 386 nm while exciting at lambda = 298 nm, mirtazapine was used as the internal standard. A careful pretreatment of wine samples was developed, using SPE with C18 cartridges (100 mg, 1 mL). The calibration curves were linear over the following concentration ranges: 0.03-5.00 ng/mL for MLT, 3-500 ng/mL for t-RSV and 1-150 ng/mL for c-RSV. The LOD values were 0.01 ng/mL for MLT, 1 ng/mL for t-RSV and 0.3 ng/mL for c-RSV. Precision data, as well as extraction yield and sample purification results, were satisfactory. Thus, the method seems to be suitable for the analysis of MLT and resveratrol isomers in wine samples. Moreover, wine total polyphenol content and antioxidant activity were evaluated.

  5. Analysis of Some Egyptian Cosmetic Samples by Fast Neutron Activation Analysis

    CERN Document Server

    Medhat, M E; Fayez-Hassan, M

    2001-01-01

    A description of D-T neutron generator (NG) is presented. This generator can be used for fast neutron activation analysis applied to determine some selected elements, especially light elements, in different materials. In our work, the concentration of the elements Na, Mg, Al, Si, K, Cl, Ca and Fe, were determined in two domestic brands of face powder by using 14 MeV neutron activation analysis.

  6. Sjuksköterskans dilemma inom psykiatrisk slutenvård - En balansgång mellan gott och ont : Sjuksköterskors erfarenheter av att lindra patienters lidande orsakat av tvångsåtgärder

    OpenAIRE

    Källbom, Annika; Mitts-Boduroglu, Maria

    2014-01-01

    Title: The nurse's dilemma in psychiatric inpatient care - a balancing act between good and evil Objective: To describe the nurse's experience of relieving patient suffering caused by coercive measures on a closed psychiatric ward . Frame of reference : The results are discussed in light of Eriksson´s theory of suffering and caring relationships Method: A qualitative analysis based on focus group discussions Results: The analysis resulted in six categories, to protect the patient , to involve...

  7. Multilocus sequence analysis of nectar pseudomonads reveals high genetic diversity and contrasting recombination patterns.

    Directory of Open Access Journals (Sweden)

    Sergio Alvarez-Pérez

    Full Text Available The genetic and evolutionary relationships among floral nectar-dwelling Pseudomonas 'sensu stricto' isolates associated to South African and Mediterranean plants were investigated by multilocus sequence analysis (MLSA of four core housekeeping genes (rrs, gyrB, rpoB and rpoD. A total of 35 different sequence types were found for the 38 nectar bacterial isolates characterised. Phylogenetic analyses resulted in the identification of three main clades [nectar groups (NGs 1, 2 and 3] of nectar pseudomonads, which were closely related to five intrageneric groups: Pseudomonas oryzihabitans (NG 1; P. fluorescens, P. lutea and P. syringae (NG 2; and P. rhizosphaerae (NG 3. Linkage disequilibrium analysis pointed to a mostly clonal population structure, even when the analysis was restricted to isolates from the same floristic region or belonging to the same NG. Nevertheless, signatures of recombination were observed for NG 3, which exclusively included isolates retrieved from the floral nectar of insect-pollinated Mediterranean plants. In contrast, the other two NGs comprised both South African and Mediterranean isolates. Analyses relating diversification to floristic region and pollinator type revealed that there has been more unique evolution of the nectar pseudomonads within the Mediterranean region than would be expected by chance. This is the first work analysing the sequence of multiple loci to reveal geno- and ecotypes of nectar bacteria.

  8. Determination of carbofuran in surface water and biological tissue by sol-gel immunoaffinity extraction and on-line preconcentration/HPLC/UV analysis.

    Science.gov (United States)

    Vera-Avila, Luz E; Márquez-Lira, Bani P; Villanueva, Marcos; Covarrubias, Rosario; Zelada, Gustavo; Thibert, Valérie

    2012-01-15

    A selective and simple analytical method for the trace level determination of carbofuran in complex environmental and biological samples was developed based on immunoaffinity extraction (IAE) followed by on-line preconcentration and HPLC/UV analysis of the purified extract. The immunosorbent for IAE was prepared by sol-gel encapsulation of monoclonal anti-carbofuran antibodies, and was fully characterized for capacity, repeatability, binding strength, binding kinetics and cross-reactivity. Method performance was evaluated with two different types of difficult samples: dam water and methanolic extracts of epithelial cervical-uterine tissue. Linear behavior and quantitative recoveries were obtained from the analysis of samples spiked with carbofuran at 0.2-4 ng/mL (dam water, 50 mL samples) and 10-40 ng/mL (biological tissue extract, 2 mL samples). RSD (n=7) and detection limits were, respectively, 10.1% (spike 0.40 ng/mL) and 0.13 ng/mL for dam water; 8.5% (spike 20 ng/mL) and 5 ng/mL for the biological tissue extract. The excellent sample purification achieved with the IAE column allows precise and accurate determination of carbofuran in complex matrices, even when using non-selective UV detection in the chromatographic analysis.

  9. Samhällsvård och välfärdsresurser : En studie av skolgång, fritid och kamratrelationer bland unga i familjehem och institutioner

    OpenAIRE

    Lagerlöf, Hélène

    2012-01-01

    The dissertation analyses access to welfare resources within the areas of schooling, leisure and peer relations for youth in out of home care. The study was conducted in three counties in mid Sweden and is a replication of the recurrent Swedish surveys of living condition of children in general populations. By using the same design, children aged 13–18 (n=272) in foster care and residential care were approached. Throughout the analysis results are compared with conditions for peers living at ...

  10. STU DY ON THE TEACHING PATTERN OF BODY BUILDI NG IN ORDINARY HIGHER LEARNING INSTITUT IONS%对普通高校健身课教学模式的探讨

    Institute of Scientific and Technical Information of China (English)

    周瑞琪

    2001-01-01

    从体育健身教育观点出发,对大学体育健身课教学模式研究的实践效果作一分析与探讨。旨在促进和推动高校健身教育体系的深入研究和有效实施。%Starting from body building education, the paper made an analysis and study of the results achieved in body huilding teaching practice. The aim is to push forward the teaching and research of body building in colleg e students′ PE class and carry out the pattern more efficiently.

  11. A Rese arch o n Determinig The Effects of Entrepreneurship Tendencies o f The Students Gettıng The Entrepreneurship Education: Sam ple of Honaz Vocational School a t Pamukkale University

    Directory of Open Access Journals (Sweden)

    Ali Soylu

    2015-09-01

    Full Text Available The concept of entrepreneurship both brings solution to the socio-economic problems of many developing countries such as unemployment, job creation, increasing the production and contributes to the national vision onan international platform. One of the most important institutions that contribute to the national vision is the universities. In this context, seeing and guiding the university students as potential entrepreneurs is of particular importance within the framework of national vision and problems.The main aim of the study is to reveal the effects of the entrepreneurship courses on the entrepreneurship orientation of the students. For this purpose, Pamukkale University conducted a field study with students taking entrepreneurship courses in Honaz Vocational School. First of all, concepts on entrepreneurship and entrepreneurial personality are described in the study. Then, statistical analysis was conducted in the context of entrepreneurial personality traits byexamining the aspects of tolerance to uncertainty, risk-taking, innovation, creativity, independence, need for achievement, relationship with people, internal locus of control among the students. Consequently, the level of impact of the entrepreneurship courses on the entrepreneurship orientation of the students was determined

  12. İLKÖĞRETİM KURUMU YÖNETİCİLERİNİN LİDERLİK TARZLARI İLE ÖĞRETMENLERİN YAŞADIKLARI YILDIRMA (MOBBİNG ARASINDAKİ İLİŞKİ

    Directory of Open Access Journals (Sweden)

    Elif DAŞÇI

    2015-04-01

    Full Text Available The aim of this research was to determine the relationship between leadership styles of elementary school principals and mobbing that teachers experience. The population of the study consisted of 60 schools and 1301 teachers working in primary schools of Ministry of National Education in 5 Central education districts of Aksaray. The sample set of research were chosen from the population with the multi-stage sampling method. The first stage was stratified sampling and the second stage was simple random sampling. 395 surveys were analyzed in the study. The Multifactor Leadership Questionnaire and Negative Acts Questionnaire were used to collect data. The data were assessed by using Pearson Product-Moment Correlation Coefficient and Multi Regression Analysis Technique. The findings of the research showed that school principals shown mid-level transformational and transactional leadership behaviours, and they were more likely to perform transformational leadership style. Teachers were victims of bullying at low level. As long as primary school principals have more transformational and transactional leadership behaviours, teachers’ mobbing experiences decrease. Idealized influence (behavior and inspirational motivation that sub-dimensions of transformational leadership style and contingent reward that subdimension of transactional leadership style are significant predictor of mobbing. Mobbing that primary school teachers experience can be explained by school principals’ leadership styles

  13. An Emprıcal Study Intend to Evaluatıon of the Service Presenting By The Groups of Accountancy Profession Towards The Relatıonship Marketıng Percept of Customers

    Directory of Open Access Journals (Sweden)

    Yusuf Kaya

    2014-12-01

    Full Text Available The concept of relationship marketing, which the companies operating in service industry adopted from past to present, but the scientific foundations of which have been laid in the last thirty years, stand out with its failure due to the increasing conditions of competition of traditional sense of marketing. The increasing number of members of accounting profession in Turkey has complicated the conditions of survival in this profession. Accordingly, it has been obligatory for the members of the profession to renew and diversify their existing marketing sense. In this study, it was put forward which factors had an impact on customers to prefer the members of accounting profession, and if these factors had a relationship with relations marketing. In the survey study whih was conducted on 755 business managers that purchased accounting service defined via convenience sampling method in Denizli region, it was questioned why they worked with the members of the profession, why they preferred them and to which points they paid attention in selection. The data were subjected to factor analysis in SPSS 15.0 software and nine basic factors were obtained. The results presented that relationshipmarketing had a significant impact on customers to prefer the members of the profession

  14. Repeated spurious elevation of serum prostate-specific antigen values solved by chemiluminescence analysis: A possible interference by heterophilic antibodies

    Science.gov (United States)

    Bayó, Miquel; Muñoz-Rodríguez, Jesús; Bellido, Jose Antonio; Abascal-Junquera, Jose María; Hannaoui, Naim; Banús, Josep Maria

    2015-01-01

    Heterophilic antibodies are human immunoglobulins directed against various animal antigens. They can produce false-positive results in the analysis of different tumor markers, including prostate-specific antigen. This interference can lead to misdiagnosis, unnecessary tests, and overtreatment in some cases. We present herein the case of a 52-year-old man with repeated spurious elevation of prostate-specific antigen, reaching levels of 108.7 ng/mL, that were suspected to be caused by heterophilic antibodies. The interference was solved by changing the analysis technique. Real values of prostate-specific antigen were less than 1 ng/mL. PMID:26568798

  15. Thе іmpаct of fіnаncіаl rеportіng of Smаll аnd Mеdіum Sіzеd Entеrprіsеs іn Economіc Dеvеlopmеnt of Bаlkаn Countrіеs

    OpenAIRE

    Artor Nuhiu; Lulzim Krasniqi; Arbër Hoti

    2012-01-01

    Thе іmportаncе of іntеrnаl аnd еxtеrnаl аccountіng аs а sourcе of іnformаtіon for ownеrs аnd mаnаgеrs of smаll еntеrprіsеs аnd thеіr dіffеrеnt stаkеholdеrs іs stеаdіly growіng. It іs of crucіаl іmportаncе thаt thе аccountіng systеms аpplіеd by smаll еntеrprіsеs mееt thеіr аctuаl nееds, provіdіng nеcеssаry іnformаtіon yеt аvoіdіng unjustіfіеd аdmіnіstrаtіvе burdеn. It іs rеcognіsеd thаt аpproprіаtе аccountіng іnformаtіon іs іmportаnt for а succеssful mаnаgеmеnt of а busіnеss whеthеr іt іs lаrg...

  16. Characterization and functional analysis of eugenol O-methyltransferase gene reveal metabolite shifts, chemotype specific differential expression and developmental regulation in Ocimum tenuiflorum L.

    Science.gov (United States)

    Renu, Indu Kumari; Haque, Inamul; Kumar, Manish; Poddar, Raju; Bandopadhyay, Rajib; Rai, Amit; Mukhopadhyay, Kunal

    2014-03-01

    Eugenol-O-methyltransferase (EOMT) catalyzes the conversion of eugenol to methyleugenol in one of the final steps of phenylpropanoid pathway. There are no comprehensive reports on comparative EOMT gene expression and developmental stage specific accumulation of phenylpropenes in Ocimum tenuiflorum. Seven chemotypes, rich in eugenol and methyleugenol, were selected by assessment of volatile metabolites through multivariate data analysis. Isoeugenol accumulated in higher levels during juvenile stage (36.86 ng g(-1)), but reduced sharply during preflowering (8.04 ng g(-1)), flowering (2.29 ng g(-1)) and postflowering stages (0.17 ng g(-1)), whereas methyleugenol content gradually increased from juvenile (12.25 ng g(-1)) up to preflowering (16.35 ng g(-1)) and then decreased at flowering (7.13 ng g(-1)) and post flowering (5.95 ng g(-1)) from fresh tissue. Extreme variations of free intracellular and alkali hydrolysable cell wall released phenylpropanoid compounds were observed at different developmental stages. Analyses of EOMT genomic and cDNA sequences revealed a 843 bp open reading frame and the presence of a 90 bp intron. The translated proteins had eight catalytic domains, the major two being dimerisation superfamily and methyltransferase_2 superfamily. A validated 3D structure of EOMT protein was also determined. The chemotype Ot7 had a reduced reading frame that lacked both dimerisation domains and one of the two protein-kinase-phosphorylation sites; this was also reflected in reduced accumulation of methyleugenol compared to other chemotypes. EOMT transcripts showed enhanced expression in juvenile stage that increased further during preflowering but decreased at flowering and further at postflowering. The expression patterns may possibly be compared and correlated to the amounts of eugenol/isoeugenol and methyleugenol in different developmental stages of all chemotypes.

  17. A novel preconcentration technique for the PIXE analysis of water

    International Nuclear Information System (INIS)

    The potential of using dried algae as a novel preconcentration technique for the analysis of water samples by PIXE was examined. The algae cells were found to contain significant levels of P and S, indicative of phosphorous- and sulfur-containing groups on the cell wall or inside the algae cells which may serve as potential binding sites for metal ions. When C. vulgaris was used on mixed metal solutions, linear responses were observed for Ag+, Ba2+, and Cd2+ in the concentration range from 10 ng/g to 1 μg/g; for Cu2+ and Pb2+ from 10 ng/g to 5 μg/g; and for Hg2+ from 10 ng/g to 10 μg/g. When S. bacillaris was used, linear responses were observed from 10 ng/g up to 10 μg/g for all of the metal cations investigated. The PIXE results demonstrated that metal binding at low concentrations involves replacement of sodium on the cell wall and that at high concentrations magnesium was also replaced. Competitive binding studies indicate that the metal ions, Ag+, Ba2+, Cd2+, Cu2+, and Pb2+, share common binding sites with binding efficiencies varying in the sequence of Pb2+>Cu2+>Ag2+>Cd2+>Ba2+. The binding of Hg2+ involved a different binding site with an increase in binding efficiency in the presence of Ag+. (orig.)

  18. The Community Health Care Demands Status and Its Influencing Factors of Elderly in Xi anni ng Ci ty%咸宁市城区老年人社区护理需求及其影响因素分析

    Institute of Scientific and Technical Information of China (English)

    张志霞; 罗菊英; 汪娟; 叶艳胜; 孔令磷

    2016-01-01

    Objectives To investigate community health care demands status and its influencing factors of elderly in Xianning City and to provide scientific reference for better community nursing services.Methods A self-designed community health care demand questionnaire was used to analyze the current status of community health care de-mands for 294 elderly in Xianning city.Results The total score of community health care demands of the elderly in Xianning was 2.89 ± 0.82. The demand of health prevention was the highest ,while the demand of disease nursing was the lowest.Multiple linear regression analysis showed that demand of disease nursing(β′=0.131) ,health pre-vention(β′=0.152) ,and overall community health nursing(β′=0.178)for elderly with higher educational level were higher than elderly with lower educational level(P<0.05). The elderly who were married showed more demand of health prevention than the elderly who were unmarried ,divorced or widowed(β′=0.154 ,P<0.05). The demand of health promotion for the younger elderly(β′= -0.128)and elderly with higher economic level(β′= 0.163)were higher than the older elderly and elderly with low economic level(P<0.05).Conclusions The elderly showed higher demands of health promotion.Community health care services should be made according to the demands of the elderly.%目的:探讨咸宁市城区老年人社区护理需求及影响因素。方法采用自制社区护理需求问卷对咸宁市城区294名老年人进行问卷调查。结果咸宁市城区老年人的社区护理总需求得分为2.89±0.82分,疾病预防需求最高,疾病护理需求最低。多元线性回归分析显示,文化程度较高者疾病护理(β′=0.131)、疾病预防(β′=0.152)和社区护理总需求(β′=0.178)得分高于文化程度较低者(P<0.05);有配偶者疾病预防得分高于未婚/离异/丧偶者(β′=0.154,P<0.05);经济收入较高(β′=0.163

  19. Multiparameter breast cancer cell image analysis for objective estimation of nuclear grade: comparison with light microscopic observational data

    Science.gov (United States)

    Berzins, Juris; Sneiders, Uldis; Plegere, Daina; Freivalds, Talivaldis; Grigalinovica, Romalda

    2000-04-01

    We performed a multi parameter image analysis assessment of breast cancer cell population nuclear grade (NG), which is regarded as one of the main prognostic factors for treatment efficacy and survival of the patients and compared it with light microscopic estimation of NG. Cytological imprint slides from 20 ductal carcinomas were stained according to Leischmann-AzureII-eosine method, and NG was estimated by light microscopic observation according to Black in Fisher's modification. Simultaneously, using specially elaborated software, in each patient 100 cancer cells were analyzed for nuclear perimeter, diameter, area, nucleolar area, and average intensity of staining. The chromatin structure was assessed using mean diameter of chromatin grains and relatively chromatic are within the nucleus. Light microscopic estimation revealed 4/15 grade 2 and 7/15 grade 3 tumors out of 15 filtrating ductal carcinomas, with 4/15 classified as intermediate between grade 2-3. Multifactoral linear correlation coefficient r equals 0.39, p < 0.001 for ductal cancer, higher NG comes with increasing nucleolar area, nuclear roundness factor, nuclear are, and chromatin area within the cell nucleus. Image analysis may yield precise information on NG as a prognostic factor in breast cancer patients.

  20. Accurate ab initio structural parameters of the diatomic and triatomic van der Waals molecules (11)BNg (X(2)Π, A(2)Σ(+)) and (11)BNg2 (X̃(2)B1), Ng = (4)He, (20)Ne, (40)Ar, (84)Kr, and (132)Xe.

    Science.gov (United States)

    Magoulas, Ilias; Papakondylis, Aristotle; Mavridis, Aristides

    2014-06-01

    The weakly interacting BNg and BNg2 molecular systems, Ng = He, Ne, Ar, Kr, and Xe, have been thoroughly studied through coupled-cluster RCCSD(T) calculations and large correlation consistent basis sets. For the BNg diatomics, the states examined are the X(2)Π and A(2)Σ(+), and the X̃(2)B1 state for the C2v BNg2 triatomics. A series of corrections render our final results reliable, judging as well from the (limited) experimental numbers available. Both BHe and BHe2 are marginally unbound, whereas the attractive interactions of the BNg X(2)Π states, where Ng = Ne, Ar, Kr, and Xe, are D0 = 19.8, 98.2, 141.9, and 209.1 cm(-1), respectively. For the BRn (Rn = radon) species, an estimated value of interaction energy D0 ≈ 280 cm(-1) is obtained by a D0 versus static polarizability (α) extrapolation. Corresponding atomization energies of the BNg2 (X̃(2)B1) molecules are AE0 = 52.0 (BNe2), 263.4 (BAr2), 384.6 (BKr2), and 576.9 (BXe2) cm(-1). PMID:24806885

  1. Environmental and occupational exposure to benzene by analysis of breath and blood.

    Science.gov (United States)

    Perbellini, L; Faccini, G B; Pasini, F; Cazzoli, F; Pistoia, S; Rosellini, R; Valsecchi, M; Brugnone, F

    1988-05-01

    Benzene exposure of chemical workers was studied, during the entire workshift, by continuous monitoring of workplace benzene concentration, and 16 hours after the end of the workshift by the measurement of alveolar and blood benzene concentrations and excretion of urinary phenol. Exposure of hospital staff was studied by measuring benzene concentrations in the alveolar and blood samples collected during the hospital workshift. Instantaneous environmental air samples were also collected, at the moment of the biological sampling, for all the subjects tested. A group of 34 chemical workers showed an eight hour exposure to benzene, as a geometric mean, of 1.12 micrograms/l which corresponded, 16 hours after the end of the workshift, to a geometric mean benzene concentration of 70 ng/l in the alveolar air and 597 ng/l in the blood. Another group of 27 chemical workers (group A) turned out to be exposed to an indeterminable eight hour exposure to benzene that corresponded, the morning after, to a geometric mean benzene concentration of 28 ng/l in the alveolar air and 256 ng/l in the blood. The group of hospital staff (group B) had a benzene concentration of 14 ng/l in the alveolar air and 269 ng/l in the blood. Instantaneous environmental samples showed that in the infirmaries the geometric mean benzene concentration was 58 ng/l during the examination of the 34 chemical workers, 36 ng/l during the examination of the 27 chemical workers (group A), and 5 ng/l during the examination of the 19 subjects of the hospital staff (group B). Statistical analysis showed that the alveolar and blood benzene concentrations in the 34 workers exposed to 1.12 microgram/l of benzene differed significantly from those in groups A and B. It was found, moreover, that the alveolar and blood benzene concentrations were higher in the smokers in groups A and B but not in the smokers in the group of 34 chemical workers. The slope of the linear correlation between the alveolar and the instantaneous

  2. Quantitative analysis of the endogenous GHB level in the hair of the Chinese population using GC/MS/MS.

    Science.gov (United States)

    Shi, Yan; Cui, Xiaopei; Shen, Min; Xiang, Ping

    2016-04-01

    Endogenous production complicates interpretation when gamma-hydroxybutyrate (GHB) is measured in hair for forensic purposes. A method capable of quantifying the endogenous concentration of GHB in human head hair was developed and validated using GC/MS/MS. Hair was digested under alkaline conditions (1 mol/L NaOH, 90 °C 10 min), and GHB-d6 was used as an internal standard. Before derivatization with BSTFA and ethyl acetate, a liquid-liquid extraction with ethyl acetate under acidic conditions was performed. GHB-TMS derivatives were detected using GC/MS/MS in the multiple-reaction monitoring mode. This method exhibited good linearity (y = 0.018x + 0.038, R(2) = 0.9998), and the limit of detection was 0.02 ng/mg. The extraction recoveries were more than 60%, and the inter-day and intra-day relative standard deviations (RSD) were less than 15%. This method has been applied for the analysis of the endogenous GHB in hair samples from 66 drug-free Chinese donors. The mean measured concentration for 0-3 cm hair was 1.93 ± 1.40 ng/mg (n = 66), and extreme values were in the range of 0.28-4.91 ng/mg. The mean male endogenous GHB level was 2.95 ng/mg (0.92-4.91 ng/mg, n = 35), while the mean female level was 0.77 ng/mg (0.28-1.95 ng/mg, n = 31). This method was applied to a forensic case for the determination of GHB in hair samples but it is hard to make a reasonable "cut off" in hair. The solution is to use each subject as his own control.

  3. Methods for analysis of citrinin in human blood and urine.

    Science.gov (United States)

    Blaszkewicz, Meinolf; Muñoz, Katherine; Degen, Gisela H

    2013-06-01

    Citrinin (CIT), produced by several Penicillium, Aspergillus, and Monascus species, has been detected as contaminant in feeds, grains, and other food commodities. CIT can co-occur with ochratoxin A (OTA), a mycotoxin also known for its nephrotoxicity, and this raises concern regarding possible combined effects. But, in contrast to OTA, data on CIT contamination in foods for human consumption are scarce, and CIT biomonitoring has not been conducted so far due a lack of suitable methods for human specimen. Thus, it was the aim of the present study to develop sensitive methods for the analysis of CIT in human blood and urine to investigate human exposure. To this end, we assessed different methods of sample preparation and instrumental analysis for these matrices. Clean-up of blood plasma by protein precipitation followed by LC-MS/MS-based analysis allowed robust detection of CIT (LOD 0.07 ng/mL, LOQ 0.15 ng/mL). For urine, sample clean-up by an immunoaffinity column (CitriTest(®)) proved to be clearly superior to SPE with RP(18) material for subsequent analysis by LC-MS/MS. For CIT and its metabolite dihydrocitrinone (HO-CIT), the LOD and LOQ determined by external calibration curves in matrix were 0.02 and 0.05 ng/mL for CIT, and those for HO-CIT were 0.05 and 0.1 ng/mL urine. The newly developed method was applied in a small pilot study: CIT was present in all plasma samples from 8 German adults, at concentrations ranging from 0.11 to 0.26 ng/mL. The molar (nM) concentrations of CIT are similar to those measured for OTA in these samples as a result of dietary mycotoxin intake. CIT was detected in 8/10 urines (from 4 adults and 6 infants) in a range of 0.16-0.79 ng/mL, and HO-CIT was present in 5/10 samples at similar concentrations. Thus, CIT is excreted in urine as parent compound and also as metabolite. These first results in humans point to the need for further studies on CIT exposure. PMID:23354378

  4. Analysis of perfluoroalkyl substances in waters from Germany and Spain.

    Science.gov (United States)

    Llorca, Marta; Farré, Marinella; Picó, Yolanda; Müller, Jutta; Knepper, Thomas P; Barceló, Damià

    2012-08-01

    Water has been identified as one of the main routes of human exposure to perfluoroalkyl substances (PFASs). This work assessed the presence of 21 PFASs along the whole water cycle using a new fast and cost effective analytical method based on an online sample enrichment followed by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). The method was validated for different types of matrices (ultrapure water, tap water and treated wastewater). The quality parameters for the 21 selected compounds presented good limits of detection (LOD) and quantification (LOQ) ranging, in general, from 0.83-10 ng/L to 2.8-50 ng/L, respectively. The method was applied to assess the occurrence of PFASs in 148 water samples of different steps along the whole water cycle, including: mineral bottled water, tap water, river water and treated effluent wastewater, from Germany to Spain. In addition, in order to prove the good performance of the online analytical method, the analysis of PFASs was carried out in parallel using a method based on offline anionic solid phase extraction (SPE) followed by LC-MS/MS. Consistent results were obtained using both approaches. The more frequently found compounds were perfluoroalkyl acids, such as the perfluorobutanoic acid which was in the 54% of the tap water samples investigated with concentrations in the range between 2.4 and 27 ng/L, the perfluoroheptanoic acid (0.23-53 ng/L) and perfluorooctanoic acid (0.16-35 ng/L), and the sulphonate perfluorooctanesulfonate (0.04-258 ng/L) which was the second more frequent compound and also the compound found in with the higher concentration. It should be remarked that the 88% of the samples analyzed presented at least one of the compounds at quantifiable concentrations. In addition, PFASs including short chain compounds were proved to be prevalent in drinking water, and the 50% of the drinking water samples showed quantifiable concentrations of PFASs. It should be said that the great majority of

  5. Uus rahvuslik ringmäng : SAPARD / Jaanus Marrandi

    Index Scriptorium Estoniae

    Marrandi, Jaanus, 1963-

    2001-01-01

    Tõenäoliselt ei jõua SAPARD-i raha ka 2001. aastal põllumeheni. Autor: Keskerakond. Parlamendisaadik. Ilmunud ka: Sakala, 25. apr. 2001, lk. 2; Nädaline, 24. apr. 2001, lk. 4; Järva Teataja, 4. mai 2001, lk. 2; Pärnu Postimees, 4. mai 2001, lk. 2; Meie Maa, 3. mai 2001, lk. 6; Võrumaa Teataja, 15. mai 2001, lk. 2; Hiiu Leht, 29. mai 2001, lk. 2

  6. LEIOMYOMA, A MAJOR CAUSE OF ABNORMAL UTERINE BLEEDI NG

    OpenAIRE

    Sreeja Rani; Sindha

    2013-01-01

    ABSTRACT: INTRODUCTION: Abnormal uterine bleeding is a common complai nt for which many patients in the perimenopausal age group un dergo hysterectomy. The objective of this study is to analyze the causes of AU B in subjects who had hysterectomy. MATERIAL AND METHODS: The pathological specimens from 100 patients who had hysterectomy for AUB between January 2012 and January 2013 we re studied based on the gross and microscopic appearance. RESULTS: L...

  7. LEIOMYOMA, A MAJOR CAUSE OF ABNORMAL UTERINE BLEEDI NG

    Directory of Open Access Journals (Sweden)

    Sreeja Rani

    2013-04-01

    Full Text Available ABSTRACT: INTRODUCTION: Abnormal uterine bleeding is a common complai nt for which many patients in the perimenopausal age group un dergo hysterectomy. The objective of this study is to analyze the causes of AU B in subjects who had hysterectomy. MATERIAL AND METHODS: The pathological specimens from 100 patients who had hysterectomy for AUB between January 2012 and January 2013 we re studied based on the gross and microscopic appearance. RESULTS: Leiomyoma alone was the most common cause of AUB (54% in patients who had hysterectomy. Leiomy oma was associated with adenomyosis in 20% cases and adenomyosis alone was seen in 10%. Endometrial cancer was found in 2% of patients. CONCLUSION: Leiomyoma is the most common indication for hysterectomy in women with AUB between 40 and 50 years. It may occur alone or may be associated with conditions like adenomyosis or endometria l polyps

  8. Karikakramäng Iraani sõjaga / Andrei Hvostov

    Index Scriptorium Estoniae

    Hvostov, Andrei, 1963-

    2007-01-01

    Autor arutleb USA võimaliku Iraagi-vastase sõja üle ning leiab, et sõja alustamine oleks vale samm. Iraan on valmis täitma võimuvaakumit, mis tekib ameeriklaste lahkumisel piirkonnast, ning on kutsunud selles osalema ka Saudi Araabia. Autori väitel püüavad Iraagist lahkuvad ameeriklased luua seal uut jõudude tasakaalu

  9. 58. "Berlinale" lõppmäng / Jaak Lõhmus

    Index Scriptorium Estoniae

    Lõhmus, Jaak

    2008-01-01

    Berliini 58. filmifestivali auhinnasaajatest : Kuldkaru võitis Brasiilia produtsendi ja režissööri Jose Padilha mängufilm "Eliitrühm", Hõbekaru USA režissööri Errol Morrise dokfilm "Tavaprotseduur". Parima režissööri Hõbekaru läks Paul Thomas Andersonile mängufilmi "Veri hakkab voolama" eest. Parima näitleja auhinna said Sally Hawkins ja Reza Naji, Alfred Baueri auhinna sai Fernando Eimbcke "Tahoe järv"

  10. Musta notsu mäng maailma finantsturgudel / Sven Meimer

    Index Scriptorium Estoniae

    Meimer, Sven

    2007-01-01

    Maailmas on tekkinud äri, kus laenu jagatakse sisuliselt laenuvõimetutele isikutele (USA-s tuntud nime all sub-prime) ning tekitatakse keerulisi laenusaajaid ja finantsettevõtteid raskustesse viivaid garantiiskeeme

  11. Checkpoint NG VPN 1firewall 1 advanced configuration and troubleshooting

    CERN Document Server

    Syngress

    2003-01-01

    Check Point Software Technologies is the worldwide leader in securing the Internet. The company''s Secure Virtual Network (SVN) architecture provides the infrastructure that enables secure and reliable Internet communications. Check Point recently announced a ground-breaking user interface that meets the industry''s next generation Internet security requirements, including simplified security management for increasingly complex environments. Built upon Check Point''s Secure Virtual Network (SVN) architecture, the Next Generation User Interface revolutionizes the way security administrators define and manage enterprise security by further integrating management functions into a security dashboard and creating a visual picture of security operations. The Next Generation User Interface delivers unparalleled ease-of-use, improved security and true end-to-end security management. Check Point''s revenues have more than doubled in each of the last two years, while capturing over 50% of the VPN market and over 40% of...

  12. Tillgång till skolbibliotek i glesbygd

    OpenAIRE

    Gällman, Johanna

    2013-01-01

    This is a two years master’s thesis in Archive, Library and Museum studies, which purpose is to find out what ideas there are about school libraries and to get an idea of how informants interpret the new Education Act, which requires all students to have access to school libraries. Another intention of this paper is to highlight the potential rural-related attitudes of the responents. The study includes the sparsely populated municipality of Krokom and is based on interviews with librarians, ...

  13. Robotteknologi i en undervisningssammenhæng

    DEFF Research Database (Denmark)

    Majgaard, Gunver

    Artiklen vil give et overblik og en vurdering over forskellige slags humant interaktive robotteknologiske læremidler. Eksemplerne, der fremlægges, er modulære robotfliser, I-BLOCKS, PicoCrickets og SmartUS. Artiklen henvender sig til folk, som vil udvikle og afprøve nye robotteknologiske læringsk......Artiklen vil give et overblik og en vurdering over forskellige slags humant interaktive robotteknologiske læremidler. Eksemplerne, der fremlægges, er modulære robotfliser, I-BLOCKS, PicoCrickets og SmartUS. Artiklen henvender sig til folk, som vil udvikle og afprøve nye robotteknologiske...

  14. Nukumäng edasijõudnutele / Heidi Aadma

    Index Scriptorium Estoniae

    Aadma, Heidi, 1976-

    2008-01-01

    Eesti Nuku- ja Noorsooteatris etendunud Tšehhis elava tšerkessi Jevgeni Ibragimovi nukulavastustest "Väga lihtne lugu" (autor Maria Lado), "Nukumängu ABC", "Mängurid" (autor Nikolai Gogol), "Oskar ja Roosa Mamma" (autor E.-E. Schmitt)

  15. ”Sammenhæng mellem kursus og praksis”

    DEFF Research Database (Denmark)

    Hansen, Bodil Winther; Bundgaard, Annette; Nysteen, Eva

    2003-01-01

    med 3 teorier, der tager udgangspunkt i praksis og lægger vægt på samspillet mellem individ og omgivelser: • Virksomhedsteori repræsenteret af Vygotsky, Leontjev og Engeström • Teorier om praksislæringved Schön og desuden er inddraget hans arbejde med organisatorisk læring foretaget i samarbejde med...

  16. Improviseeritud lugu ja zhanriga mäng / Donald Tomberg

    Index Scriptorium Estoniae

    Tomberg, Donald

    2003-01-01

    Eesti komöödiafilm "Vanad ja kobedad saavad jalad alla" : režissöör Rando Pettai : operaator Mait Mäekivi : näitlejad Henrik Normann, Madis Milling, Robert Gutman jt. : muusika - ansambel Genialistid : Ruut Pictures 2003

  17. Language Learnıng Strategy Preferences of Turkish Students

    OpenAIRE

    Selma DENEME

    2008-01-01

    A learning style is a preferred way of acquiring knowledge and processing information. Learning styles may differ depending on gender, age, or culture. Niles (1995) in his study “Cultural Differences in Learning Motivation and Learning Strategies” studied the cultural impact on learning strategies and found considerable differences between culturally different students. Providing evidence for the relationship between culture and learning strategy preference and use, this study aims at examini...

  18. IPO. Mäng suure orkestriga / Tiiu Pedaja, Gerd Laub

    Index Scriptorium Estoniae

    Pedaja, Tiiu

    2006-01-01

    Aktsiate avaliku pakkumise ehk IPO korraldamisest. Lisad: Millal on õige aeg?; Milline ettevõte sobib avalikuks pakkumiseks?; Milline avalik pakkumine on edukas?; Kuidas IPO-l aktsiaid jaotatakse? Skeemid: Aktsia hinna kujunemine avaliku pakkumise käigus; Järelturu aktsiahinda mõjutavad tegurid

  19. Nicanor Abelardo: Tagapagbunsod ng Modernismo sa Sining Musika sa Pilipinas

    Directory of Open Access Journals (Sweden)

    Ramon P. Santos

    2001-12-01

    Full Text Available The twentieth century has borne witness to some of the great changes in the musical art of Europe and the West. The historical events of the past century in economics and education, the speedy spread of intellectualization and the achievements in science all contributed to the creation of new perspectives in culture and the arts. These perspectives, which were reactions to the aesthetics of the time and to the accepted styles of expression, led to a major movement called "modernism." The various directions and views which formed the major characteristics of modernism in music came together under the aegis of freedom, freedom from the old ways. This freedom was seen as a departure from emotion and sentimentalism as well as emancipation from the long imprisonment in the system of tonal harmony, which in turn suppressed emotional breadth and human thought. While studying in America, Nicanor Abelardo embraced contemporary philosophy and techniques. This brought about a modern quality to his works during his last years. This article is an initial study of this rapid and dramatic metamorphosis in the art of NIcanor Abelardo.

  20. "Klaaspärlimäng" kolis Tartusse

    Index Scriptorium Estoniae

    2008-01-01

    Anne Veskilt ilmuvast singlist "Kallis Eestimaa". Tartu laululava muruplatsil toimuvast sõltumatu muusika festivalist Plink Plonk. Viljandi Folgist. Muusikavõrgustik Last.fm viis ilma plaadifirmata bändidele sisse autoritasude süsteemi, mille alusel muusikud saavad raha oma lugude pealt, mis Last.fm-i muusikakogusse lisatakse ja seal kuulatakse

  1. Kollisioner igång till hösten

    CERN Multimedia

    Hellman, STen

    2009-01-01

    "Der började så bra. Den 10 september var utannonserad som dagen då CERNs nya accelerator LHC för första gången skulle ta emot protoner och losta dem ett helt varv runt den 27 km långa acceleratorringen" (3 pages)

  2. Kriisikoldeid kustutab (teatri)mäng / Rait Avestik

    Index Scriptorium Estoniae

    Avestik, Rait, 1974-

    2010-01-01

    Itaallaste Nino d’Introna ja Giacomo Ravicchio teksti põhjal valminud seiklusloost „Robinson & Crusoe”, tõlkinud Kirke Org, lavastanud Aare Toikka. Esietendus 27. nov. 2009 VAT-teatris. Mängivad Meelis Põdersoo ja Ago Soots

  3. Biennaal ehk mäng neljale keelele / Johannes Saar

    Index Scriptorium Estoniae

    Saar, Johannes, 1965-

    2001-01-01

    49. Veneetsia biennaali korraldusest, probleemidest, kontseptsioonist. Loetletud preemiasaajad - peapreemiad, rahvuspaviljoni Kuldlõvi, Kuldlõvi tänapäevakunsti meistritele ja noore kunstniku preemiad

  4. Draped heterogeneity, forced uniformity: when agro-environmental policies drive family development: The U Minh Thượng forest reserve, (Mekong delta, Vietnam Hétérogénéité cachée, uniformité forcée : quand les politiques agro-environnementales guident le développement familial – Cas de la réserve forestière de U Minh Thượng (delta du Mékong, Vietnam Heterogeneidad oculta, uniformidad forzada: cuando las políticas agro-ambientales impulsan el desarrollo familiar. La reserva del bosque de U Minh Thượng (delta del Mekong, Vietnam

    Directory of Open Access Journals (Sweden)

    Mehdi Saqalli

    2011-04-01

    Full Text Available The U Minh Thượng natural reserve was created during the 1990s with a surrounding buffer zone where 3,500 households have been settled between 1992 and 1995, each household on a lot of 4 hectares. From a social point of view, the settlers were selected as 'poor people' or war veterans. A social discrimination was slowly built in favour of the latter. Despite an apparent homogeneity, the environment shows slight variations which has huge effects on the potential of the lots. From 1992 to 1998, the provincial government applied uniformly different management policies, despite the fact that this repeated "new deal" of the variability among households and lots, produced various outcomes from total destruction to a real support to households. Finally, the support of international environmental non-governmental organizations enhanced a pro-nature hard-line that has affected farmers' livelihoods. This 'real world' social experiment enhances the necessity to at least understand the needs and constraints at a lower scale, especially for such a vast area.La réserve naturelle de U Minh Thượng a été créée dans les années 1990, ceinturée par une zone tampon où ont été installés 3 500 ménages entre 1992 et 1995. Chaque ménage a reçu un lot de 4 hectares. Sur le plan social, les colons ont été sélectionnés parmi les « pauvres » ou les anciens combattants. Une différenciation sociale s’est progressivement instaurée en faveur de ces derniers. Malgré une homogénéité apparente, le milieu physique montre de légères variations, qui ont des répercussions considérables sur le potentiel des lots. De 1992 à 1998, le gouvernement provincial a appliqué différentes politiques d’aménagement de façon uniforme, bien que cette « nouvelle donne » répétée de la variabilité entre les ménages et les terrains ait des effets mitigés, pouvant aller d’un échec total à un réel soutien aux familles. Enfin, le soutien d

  5. Yang Pagbul’lók-bul’lok: Varyasyon ng Wikang Mandaya sa Ilang Munisipalidad ng Davao Oriental

    Directory of Open Access Journals (Sweden)

    Dr. Raymund M. Pasion

    2015-05-01

    Full Text Available The study aimed to find out the lexical, phonological, and morphological variations of the Mandaya language in the municipalities of Manay, Caraga, Baganga and Cateel in the province of Davao Oriental. This research was conducted to meet the following objectives: (1 To present the terminologies used in the four municipalities based on the Indigenous Knowledge System and Practices of Mandaya in cultural farming, hunting, fishing and animal husbandry. (2 To identify the variations in the Mandaya language based on lexicon, phonology and morphology. (3 To show the distinct linguistic feature in the Mandaya language that is different from Filipino language based on lexicon, phonology and morphology. Qualitative design, descriptive and indigenous methods were used in gathering and analyzing the data. Purposive combination and snow-ball sampling were utilized in choosing the informants. It was found out in the study that there was a variation of the Mandaya language in the four municipalities. In the lexical aspect, there were three evident categories: the different in form; the slightly similar in form; and the same in form but different in pronunciation. While in the phonological aspect, it also showed three variations; the phoneme /h/; the phoneme /ᵊ/ (swa; and the glide l or /l−l/. On the other hand, in the morphological aspect, the researcher observed two things: the affix and morphological phenomena occurring in the words in different dialects in Mandaya. In general, the occurrence of variations in lexical, phonological and morphological aspects in the Mandaya language was inevitable, and this was caused by the three factors: the linguistics, sociolinguistics and psychological.

  6. Thе іmpаct of fіnаncіаl rеportіng of Smаll аnd Mеdіum Sіzеd Entеrprіsеs іn Economіc Dеvеlopmеnt of Bаlkаn Countrіеs

    Directory of Open Access Journals (Sweden)

    Artor Nuhiu

    2012-04-01

    Full Text Available Thе іmportаncе of іntеrnаl аnd еxtеrnаl аccountіng аs а sourcе of іnformаtіon for ownеrs аnd mаnаgеrs of smаll еntеrprіsеs аnd thеіr dіffеrеnt stаkеholdеrs іs stеаdіly growіng. It іs of crucіаl іmportаncе thаt thе аccountіng systеms аpplіеd by smаll еntеrprіsеs mееt thеіr аctuаl nееds, provіdіng nеcеssаry іnformаtіon yеt аvoіdіng unjustіfіеd аdmіnіstrаtіvе burdеn. It іs rеcognіsеd thаt аpproprіаtе аccountіng іnformаtіon іs іmportаnt for а succеssful mаnаgеmеnt of а busіnеss whеthеr іt іs lаrgе or smаll.At EU lеvеl, аccountіng lеgіslаtіon іs іn plаcе for lіstеd compаnіеs, і.е. thе Intеrnаtіonаl Accountіng Stаndаrds/Intеrnаtіonаl Fіnаncіаl Rеportіng Stаndаrds аnd for non-lіstеd lіmіtеd lіаbіlіty compаnіеs, thе Fourth аnd thе Sеvеnth Compаny Lаw Dіrеctіvеs і.е. thе Accountіng Dіrеctіvеs. Howеvеr, аt EU lеvеl thеrе іs no аccountіng lеgіslаtіon аpplіcаblе to thosе еntеrprіsеs whіch аrе not lіstеd or аrе not lіmіtеd lіаbіlіty compаnіеs; іn most cаsеs wе would bе rеfеrrіng to smаll еntеrprіsеs.Bеcаusе of thе іmportаncе of аpproprіаtе аccountіng іnformаtіon for ownеrs аnd mаnаgеrs of smаll еntеrprіsеs аnd thеіr dіffеrеnt stаkеholdеrs, іt іs consіdеrеd іmportаnt to аnаlysе thе vаrіous аccountіng systеms аpplіеd іn Mеmbеr Stаtеs іn thе cаsе of non-rеgulаtіon аt EU lеvеl.

  7. Environmental analysis of alcohol ethoxylates and nonylphenol ethoxylate metabolites by ultra-performance liquid chromatography-tandem mass spectrometry.

    Science.gov (United States)

    Lara-Martín, Pablo A; González-Mazo, Eduardo; Brownawell, Bruce J

    2012-03-01

    Surfactants and their metabolites can be found in aquatic environments at relatively high concentrations compared with other micropollutants due in part to the exceptionally large volumes produced every year. We have focused our attention here on the most widely used nonionic surfactants, alcohol ethoxylates (AEOs), and on nonylphenol ethoxylate (NPEO) degradation products (short-chain nonylphenol ethoxylates, NP1-3EO, nonylphenol, NP, and nonylphenol ethoxycarboxylates, NP1-2EC), which are endocrine-disrupting compounds. Our main objective in this work was to develop a methodology aimed at the extraction, isolation, and improved analysis of these analytes in environmental samples at trace levels. Extraction recoveries of target compounds were determined for sediment samples after ultrasonic extraction and purification using HLB or C18 solid-phase extraction minicolumns. Recovery percentages were usually between 61 and 102% but were lower for longer AEO ethoxymers. Identification and quantification of target compounds was carried out using a novel ultra-performance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS-MS) approach, a combination that provides higher sensitivity and faster analysis than prior methods using conventional high-performance liquid chromatography-mass spectrometry. Limits of detection were usually below 0.5 ng/g, being higher for monoethoxylate species (>5 ng/g) because of poor ionization. The method was used for analyzing surface sediment samples collected at Jamaica Bay (NY) in 2008. The highest values (28,500 ng/g for NP, 4,200 ng/g for NP1-3EO, 22,400 ng/g for NP1-2EC, and 1,500 ng/g for AEOs) were found in a sampling station from a restricted water circulation area that is heavily impacted by wastewater discharges. PMID:22002557

  8. Bottled water: analysis of mycotoxins by LC-MS/MS.

    Science.gov (United States)

    Mata, A T; Ferreira, J P; Oliveira, B R; Batoréu, M C; Barreto Crespo, M T; Pereira, V J; Bronze, M R

    2015-06-01

    The presence of mycotoxins in food samples has been widely studied as well as its impact in human health, however, information about its distribution in the environment is scarce. An analytical method comprising a solid phase extraction procedure followed by liquid chromatography tandem mass spectrometry analysis was implemented and validated for the trace analysis of mycotoxins in drinking bottled waters. Limits of quantification achieved for the method were between 0.2ngL(-1) for aflatoxins and ochratoxin, and 2.0ngL(-1) for fumonisins and neosolaniol. The method was applied to real samples. Aflatoxin B2 was the most frequently detected mycotoxin in water samples, with a maximum concentration of 0.48±0.05ngL(-1) followed by aflatoxin B1, aflatoxin G1 and ochratoxin A. The genera Cladosporium, Fusarium and Penicillium were the fungi more frequently detected. These results show that the consumption of these waters does not represent a toxicological risk for an adult. PMID:25624256

  9. Bottled water: analysis of mycotoxins by LC-MS/MS.

    Science.gov (United States)

    Mata, A T; Ferreira, J P; Oliveira, B R; Batoréu, M C; Barreto Crespo, M T; Pereira, V J; Bronze, M R

    2015-06-01

    The presence of mycotoxins in food samples has been widely studied as well as its impact in human health, however, information about its distribution in the environment is scarce. An analytical method comprising a solid phase extraction procedure followed by liquid chromatography tandem mass spectrometry analysis was implemented and validated for the trace analysis of mycotoxins in drinking bottled waters. Limits of quantification achieved for the method were between 0.2ngL(-1) for aflatoxins and ochratoxin, and 2.0ngL(-1) for fumonisins and neosolaniol. The method was applied to real samples. Aflatoxin B2 was the most frequently detected mycotoxin in water samples, with a maximum concentration of 0.48±0.05ngL(-1) followed by aflatoxin B1, aflatoxin G1 and ochratoxin A. The genera Cladosporium, Fusarium and Penicillium were the fungi more frequently detected. These results show that the consumption of these waters does not represent a toxicological risk for an adult.

  10. Сеть DISN как прототип сети связи гражданской обороны NG112

    OpenAIRE

    Шнепс-Шнеппе М.А.; Селезнев С.П.; Куприяновский В.П.

    2016-01-01

    Настоящая работа посвящена обсуждению стратегии развития телекоммуникаций в мире и поощрению отечественных разработок. В статье ставится задача – воспользоваться методическими материалами по оборонной сети DISN для разработки российской сети связи гражданской обороны нового поколения NG112 (NG – Next Generation). Показаны трудности, которые имеются на пути перехода от коммутации каналов (КК) к коммутации пакетов (КП): на правительственной сети сохраняются каналы ISDN, не определены сроки отка...

  11. Anion exchange SPE and liquid chromatography-tandem mass spectrometry in GHB analysis.

    Science.gov (United States)

    Elian, Albert A; Hackett, Jeffery

    2011-12-01

    In this study, the extraction of γ-hydroxybutyrate (GHB) from urine using solid-phase extraction (SPE) is described. SPE was performed on anion exchange columns after samples of urine had been diluted with de-ionized water. After application of the diluted samples containing GHB-d(6) as an internal standard, the sorbent was washed with deionized water and methanol and dried. The GHB was eluted from the SPE column with a solvent consisting of methanol containing 6% glacial acetic acid. The eluent was collected, evaporated to dryness, and dissolved in mobile phase (100 μL) for analysis by liquid chromatography-tandem mass spectrometry (LC-MS/MS) in negative multiple reaction monitoring (MRM) mode. Liquid chromatography was performed in gradient mode employing a biphenyl column and a mobile phase consisting of acetontitrile (containing 0.1% formic acid) and 0.1% aqueous formic acid. The total run time for each analysis was less than 5 min. The limits of detection/quantification for this method were determined to be 50 and 100 ng/mL, respectively. The method was found to be linear from 500 ng/mL to 10,000 ng/mL (r(2)>0.995). The recovery of GHB was found to be greater than 75%. In this report, results of authentic urine samples analyzed for GHB by this method are presented. GHB concentrations in these samples were found to be range from less than 500 ng/mL to 5110 ng/mL.

  12. Analysis of multiple anticoagulant rodenticides in animal blood and liver tissue using principles of QuEChERS method.

    Science.gov (United States)

    Vudathala, Daljit; Cummings, Margaret; Murphy, Lisa

    2010-06-01

    A quick and easy method for the analysis of anticoagulant rodenticides in blood or tissue using principles of dispersive solid-phase extraction (dSPE), commonly known as QuEChERS (short for quick, easy, cheap, effective, rugged, and safe), was developed. Briefly, a combination of magnesium sulfate, PSA, florisil, and basic alumina was used to cleanup blood samples. Further, to cleanup liver tissue samples, C(18) sorbent was included along with the previously mentioned. The samples were analyzed using high-performance liquid chromatography equipped with a reversed-phase C(18) column (150 x 4.6 mm, 5-microm particle size) and a UV and fluorescence detector. The mobile phase consisted of 0.03 M tetrabutylammonium hydroxide (TBA) adjusted to pH 7/methanol (1:1, v/v) as solvent A and methanol as solvent B in a gradient run. The method detection limit was as low as 10 ng/mL for brodifacoum and difenacoum in blood and 10 ng/g in liver; 50 ng/mL for bromadiolone, difethialone, and chlorphacinone in blood and similarly 50 ng/g in liver; and 100 ng/mL for coumafuryl, pindone, warfarin, and diphacinone in blood and 100 ng/g in liver samples. A number of clinical samples of both blood and liver were analyzed; the comparison of this modified QuEChERS and traditional solid-phase extraction data was found to be in close agreement. This method resulted in drastic reduction in processing time and solvent cost both in terms of consumption and disposal, thus making it an attractive alternative to the traditional solid-phase extraction. PMID:20529461

  13. Analysis of ochratoxin A blood levels in bladder cancer cases and healthy persons from Pakistan.

    Science.gov (United States)

    Aslam, Muhammad; Rivzi, S Abidul Hassan; Beg, Anwer Ejaz; Blaszkewicz, Meinolf; Golka, Klaus; Degen, Gisela H

    2012-01-01

    The mycotoxin ochratoxin A (OTA), a well-known human nephrotoxic and carcinogenic agent, is a public health concern in many countries. Exposure is assessed by means of mycotoxin analysis in food commodities and by human biomonitoring of OTA in blood samples. Data available from several European countries and some studies in Africa, Asia, and the Americas indicate frequent detection of OTA. Thus far, data from developing countries that compare blood levels in healthy and diseased individuals are scarce. Thus, the aim of this investigation was to determine OTA levels in blood samples of bladder cancer patients (n = 96) and healthy controls (n = 31) from Pakistan. OTA in blood plasma was analyzed after extraction by high-performance liquid chromatography (HPLC) with fluorescence detection. Among samples of 87 cancer patients and 30 controls, 92% in total contained quantifiable amounts of OTA. In bladder cancer cases the median OTA concentration was 0.19 ng/ml (mean 0.296; range: 0.03 to 3.41 ng/ml), and in healthy controls the median OTA was 0.19 ng/ml (mean 0.3; range: 0.04 to 1.24 ng/ml). The OTA levels found in the Pakistanian cohorts were comparable to those reported previously for the general population in the European Union. In conclusion, OTA is not likely to play a major role in the etiology of bladder cancer in the Karachi cohort, at least as the sole risk factor.

  14. Deconstruction-Construction-Reconstruction:On Chinese Americans' Ethnic Identification in Fae Myenne Ng's Bone%解构-建构-重建--伍慧明《骨》中美国华裔的族裔身份探寻历程

    Institute of Scientific and Technical Information of China (English)

    周燕萍

    2014-01-01

    身份问题对于散居海外、生存在多重文化夹缝中的美国华人是一个无法规避的问题,美籍华裔作家伍慧明首部作品直接以“骨”命名,体现了美国华裔族裔身份认同过程中的取向。以后殖民身份理论为支点,分析《骨》中美国华裔在族裔身份探寻过程中所经历的中式身份解构、美式身份建构及杂糅式身份的重建历程。%Identity has been the top concern for diaspora Chinese Americans who are under multi-cultures. Chinese American writer Fae Myenne Ng entitles her debut Bone to excavate Chinese Americans'ethnic identification orien-tation. Using post-colonial identity as theoretical foundation, this thesis intends to analyze Chinese Americans' de-construction of Chinese identity, construction of American identity and reconstruction of a hybrid identity.

  15. Influence of presenilin 1 overexpression on endogenous tau phosphorylation in neuroblastoma cells%早老蛋白-1过度表达对神经母细胞瘤NG-108的tau蛋白磷酸化的影响

    Institute of Scientific and Technical Information of China (English)

    陈波; 程敏; 王寅; 孙凤艳; 朱粹青

    2006-01-01

    目的 观察早老蛋白-1(Presenilin-1,PS1)的过度表达对tau蛋白磷酸化的影响.方法 在NG-108细胞上转染不同的PS1质粒, 利用免疫双标,免疫印记, 免疫细胞化学,MTT,Western blot 等方法, 观察早老蛋白-1的过度表达对tau蛋白磷酸化的影响.结果 免疫双标显示,在散发性AD(sporadic Alzheimer disease)患者脑内, PS1主要分布于神经元,并与磷酸化tau蛋白部分共存.免疫印迹显示, 与空质粒组相比,转染野生型PS1组和转染一种家族性AD(familial Alzheimer disease,FAD)突变型早老蛋白-1(mPS1)质粒组的NG-108细胞中磷酸化的tau蛋白均增加.MTT方法未检测到这两组的转染细胞与对照组有显著差异.用Confocal显微镜观察PS1-EGFP质粒转染组, 发现在转染后早期12 h,PS1-EGFP主要分布在细胞膜表面或细胞器上,随后PS1-EGFP主要在细胞浆中弥散分布.同时,免疫细胞化学检测显示,部分PS1-EGFP 转染细胞中有明显的tau蛋白磷酸化,并且这些磷酸化tau蛋白能与PS1-EGFP蛋白一起形成聚集体.在给予磷酸酯酶抑制剂岗田酸后, PS1-EGFP 转染细胞比未转染细胞含有更多的磷酸化的tau蛋白.结论 野生型PS1可能参与了散发性阿茨海默氏病中的tau蛋白的病理改变.%Purpose To study the effect of overexpressing either wild type or a familial Alzheimer disease mutant presenilin 1 (mPS1) on tau phosphorylation in neuroblastoma NG-108 cells. Methods Three different plasmids transfected NG-108 cells respectively. Immunostaining and confocal microscopic technique were used to study the distribution of presenilin 1 and phosphorylated tau. Immunoblot test was applied to investigate the change of tau phosphorylation. Results Immunostaining showed that in brain of sporadic Alzheimer disease, PS1 mainly distributed in neuron and partially colocalized with the phosphorylated tau. Immunoblot tests showed that the cells transected either wild type PS1 or mPS1 contained more

  16. Analysis of testosterone in human urine using molecularly imprinted solid-phase extraction and corona discharge ion mobility spectrometry.

    Science.gov (United States)

    Mirmahdieh, Shiva; Mardihallaj, Azam; Hashemian, Zahra; Razavizadeh, Jalal; Ghaziaskar, Hassan; Khayamian, Taghi

    2011-01-01

    Analysis of testosterone was accomplished using corona discharge ion mobility spectrometry. Molecular imprinted polymer was used for the extraction and pre-concentration of testosterone. Analytical parameters including precision, dynamic range and detection limit were obtained. The linear dynamic range was from 10 to 250 ng/mL and the limit of detection was 0.9 ng/mL. The proposed method was used for analysis of testosterone in urine samples. A urine sample from a 3-year-old girl was used as the blank. The RSD was below 10%. The obtained results from the method were also compared with the standard method for analysis of testosterone using SPE-HPLC analysis. The results demonstrate the accuracy of the method. PMID:21171183

  17. Analysis of biomagnification of persistent organic pollutants in the aquatic food web of the Mekong Delta, South Vietnam using stable carbon and nitrogen isotopes.

    Science.gov (United States)

    Ikemoto, Tokutaka; Tu, Nguyen Phuc Cam; Watanabe, Michio X; Okuda, Noboru; Omori, Koji; Tanabe, Shinsuke; Tuyen, Bui Cach; Takeuchi, Ichiro

    2008-05-01

    The present study elucidated the biomagnification profiles of persistent organic pollutants (POPs) through a tropical aquatic food web of Vietnam based on trophic characterization using stable nitrogen analysis. Various biological samples collected from the main stream of the Mekong Delta were provided for the analysis for both POPs, and stable nitrogen and carbon isotope ratios. Of the POPs analyzed, dichlorodiphenyltrichloroethane and its metabolites (DDTs) were the predominant contaminants with concentrations ranging from 0.058 to 12 ng/g wet weight, followed by polychlorinated biphenyls (PCBs) at 0.017-8.9 ng/g, chlordane compounds (CHLs) at 0.0043-0.76 ng/g, tris-4-chlorophenyl methane (TCPMe) at N.D.-0.26 ng/g, hexachlorocyclohexane isomers (HCHs) at N.D.-0.20 ng/g and hexachlorobenzene (HCB) at 0.0021-0.096 ng/g. Significant positive increases of concentrations in DDTs, CHLs, and TCPMe against the stable nitrogen ratio (delta(15)N) were detected, while, concentrations of HCHs and HCB showed no significant increase. The slopes of the regression equations between the log-transformed concentrations of these POPs and delta(15)N were used as indices of biomagnification. The slopes of the POPs for which positive biomagnification was detected ranged from 0.149 to 0.177 on a wet weight basis. The slopes of DDTs and CHLs were less than those reported for a marine food web of the Arctic Ocean, indicating that less biomagnification had occurred in the tropical food web. Of the isomers of CHLs, unlike the studies of the Arctic Ocean, oxychlordane did not undergo significant biomagnification through the food web of the Mekong Delta. This difference is considered to be due to a lack of marine mammals, which might metabolize cis- and trans-chlordane to oxychlordane, in the Mekong Delta ecosystem. The biomagnification profile of TCPMe is reported for the first time in the present study. PMID:18313720

  18. A Cross Sectional Analysis of Gonococcal and Chlamydial Infections among Men-Who-Have-Sex-with-Men in Cape Town, South Africa.

    Directory of Open Access Journals (Sweden)

    Kevin Rebe

    Full Text Available Men-who-have-sex-with-men (MSM are at high risk of HIV and sexually transmitted infection (STI transmission. Asymptomatic STIs are common in MSM and remain undiagnosed and untreated where syndromic management is advocated. Untreated STIs could be contributing to high HIV rates. This study investigated symptomatic (SSTI and asymptomatic STIs (ASTIs in MSM in Cape Town.MSM, 18 years and above, were enrolled into this study. Participants underwent clinical and microbiological screening for STIs. Urine, oro-pharyngeal and anal swab specimens were collected for STI analysis, and blood for HIV and syphilis screening. A psychosocial and sexual questionnaire was completed. STI specimens were analysed for Neisseria gonorrhoeae (NG and Chlamydia trachomatis (CT infection.200 MSM were recruited with a median age of 32 years (IQR 26-39.5. Their median number of sex partners within the last year was 5 (IQR 2-20. 155/200 (78% reported only male sex partners while 45/200 (23% reported sex with men and women. 77/200 (39% reported transactional sex. At enrolment, 88/200 (44% were HIV positive and 8/112 (7% initially HIV-negative participants seroconverted during the study. Overall, 47/200 (24% screened positive for either NG or CT. There were 32 MSM (16% infected with NG and 7 (3.5% of these men had NG infections at two anatomical sites (39 NG positive results in total. Likewise, there were 23 MSM (12% infected with CT and all these men had infections at only one site. Eight of the 47 men (17% were infected with both NG and CT. ASTI was more common than SSTI irrespective of anatomical site, 38 /200 (19% versus 9/200 (5% respectively (p<0.001. The anus was most commonly affected, followed by the oro-pharynx and then urethra. Asymptomatic infection was associated with transgender identity (OR 4.09 CI 1.60-5.62, ≥5 male sex partners in the last year (OR 2.50 CI 1.16-5.62 and transactional sex (OR 2.33 CI 1.13-4.79 but not with HIV infection.Asymptomatic STI was

  19. Proteomics Analysis for Finding Serum Markers of Ovarian Cancer

    Directory of Open Access Journals (Sweden)

    Yushan Cheng

    2014-01-01

    Full Text Available A combination of peptide ligand library beads (PLLB and 1D gel liquid chromatography-mass spectrometry/mass spectrometry (1DGel-LC-MS/MS was employed to analyze serum samples from patients with ovarian cancer and from healthy controls. Proteomic analysis identified 1200 serum proteins, among which 57 proteins were upregulated and 10 were downregulated in the sera from cancer patients. Retinol binding protein 4 (RBP4 is highly upregulated in the ovarian cancer serum samples. ELISA was employed to measure plasma concentrations of RBP4 in 80 samples from ovarian cancer patients, healthy individuals, myoma patients, and patients with benign ovarian tumor, respectively. The plasma concentrations of RBP4 ranging from 76.91 to 120.08 ng/mL with the mean value 89.13±1.67 ng/mL in ovarian cancer patients are significantly higher than those in healthy individuals (10.85±2.38 ng/mL. Results were further confirmed with immunohistochemistry, demonstrating that RBP4 expression levels in normal ovarian tissue were lower than those in ovarian cancer tissues. Our results suggested that RBP4 is a potential biomarker for diagnostic of screening ovarian cancer.

  20. Correlation analysis of plasma brain natriuretic peptide and recent prognosis in patients with acute pulmonary embolism

    Institute of Scientific and Technical Information of China (English)

    Hui-Zhao Liu

    2016-01-01

    Objective:To study the correlation analysis of plasma brain natriuretic peptide (BNP) and recent prognosis in patients with acute pulmonary embolism (APE).Methods:98 cases of patients with APE were selected as the research subjects from January 2012 to January 2015, all patients were divided into BNP<100 ng/L group (n=41 cases) and a BNP≥100 ng/L group (n=57 cases) according to the plasma BNP level. Collection of two groups of patients with clinical data and related laboratory examination indexes, record the incidence rate of two groups of patients with clinical adverse events, comparing the difference between the two groups.Results:With the BNP<100 ng/L group comparison, BNP acuity 100 ng/L group patients' heart rate (HR) partial fast, systolic blood pressure (SBP) is low, the number of people in the clinical manifestations of shortness of breath and syncope, blood gas analysis in the oxygen partial pressure (PaO2) are low, the plasma D-dimer is on the high side, the proportion of a massive embolism is higher (P<0.05); The BNP 100 ng/L or group of patients with cardiac shock, tracheal intubation and cardiopulmonary resuscitation (CPR) and the recent clinical adverse events such as death is more than the BNP<100 ng/L group (P<0.05); Logistic regression analysis suggest the plasma BNP levels (OR=1.137,P<0.05) were independent risk factors for the development of clinical adverse events in patients with APE. ROC curve analysis showed that plasma BNP in patients with predicted area under the curve of the clinical adverse events was 0.841 (95%CI=0.585–0.937,P<0.05), the sensitivity and specificity were 87.3% and 79.8% respectively.Conclusions:Early serum BNP level and APE patients is closely related to the severity and prognosis of recent, early detection of plasma BNP helps in evaluating the prognosis of patients with early admission, to guide treatment of clinical significance.

  1. Amitriptyline poisoning of a baby: how informative can hair analysis be?

    Science.gov (United States)

    Allibe, Nathalie; Eysseric-Guerin, Hélène; Kintz, Pascal; Bartoli, Mireille; Bost-Bru, Cécile; Grenier, Florian; Scolan, Virginie; Stanke-Labesque, Françoise

    2015-04-01

    We reported a case of a 6-month-old baby girl who was hospitalized in the pediatric emergency for central nervous system disorders then coma. Toxicology analysis showed the presence of amitriptyline (AMI) and its metabolite nortriptyline (NOR) in blood and urine of the baby. Additional investigations suggested a shaken baby syndrome. Given the family context, a judge ordered hair tests for both the child and his parents to document drug exposure. A liquid chromatography tandem mass spectrometric (LC-MS/MS) method was then developed to quantify AMI and NOR in hair. After decontamination and segmentation, 20 mg of hair was incubated overnight at 55 °C in methanol (MeOH). The LC-MS/MS method used an online solid phase extraction and the analysis was performed using two transitions per compound. The LOQ and LOD for the two compounds were estimated at 0.0075 ng/mg and 0.005 ng/mg respectively. All hair segments tested for both parents were negative. For the baby two strands of hair were collected one day after the acute intoxication for the first and 5 weeks later for the second. The first strand was not decontaminated before analysis to avoid losing specimen. The high and relatively homogenous concentrations of AMI (with a range of value from 6.65 to 9.69 ng/mg) and NOR (with a range of value from 7.12 to 8.96 ng/mg) measured suggested that contamination could have occurred. The analysis of the second strand after decontamination allowed to detect AMI and NOR in all hair segments. The obtained values varied between 0.54 and 1.41 ng/mg for AMI and between 1.26 and 4.00 ng/mg for NOR. These results supported the hypothesis of a chronic exposure during several months before hair collection with regular increase. However a single overdose could not be totally excluded. The interpretation of results must take into account the pharmacological and physiological parameters of hair of the children. PMID:25676714

  2. 河池市金城江区2010-2014年新生儿出生缺陷监测分析%Birth Defects Monitoring Analysis of Jin Chengjia Ng area in Hechi City from 2010 to 2014

    Institute of Scientific and Technical Information of China (English)

    李娟; 兰仙朵

    2015-01-01

    Objective To monitor birth defects of Jin Chengjiang area in Hechi city from 2010 to 2014. Methods Selected birth defects monitoring data of Jin Chengjiang area in Hechi city from 2010 to 2014. Results The birth defect rate of Jin Chengjiang area was 18.85 ‰ from 2010 to 2014, there is an upward trend in the incidence of birth defects. Male’s birth defects incidence rate was higher than baby?daughter, birth defects rate of pregnant women beyond 35 ages was highest. The first there disease of birth defects rate were congenital heart disease, congenital hydrocephalus and merger of cleft lip cleft palate respectively. The mainly diagnosis basis was clinical diagnosis and B ultrasonic diagnosis. Conclusion Perinatal birth defects of Jin Chengjiang district was higher than the national level, birth defects rate was present an upward trend year by year, and need to pay a attention.%目的:对河池市金城江区2010—2014年出生缺陷新生儿进行监测分析。方法收集河池市金城江区2010—2014年出生缺陷监测资料。结果2010—2014年金城江区新生儿缺陷发生率为18.85‰,出生缺陷儿的发生率呈逐年上升的趋势。男性围产儿的出生缺陷发生率高于女性,孕妇年龄≥35岁发生出生缺陷率最高。出生缺陷发生率位于前三的分别为先天性心脏病、先天性脑积水及唇裂合并腭裂。主要依据为临床诊断和B超诊断。结论金城江区围产儿出生缺陷高于全国水平,出生缺陷发生率呈逐年上升的趋势,需要引起重视。

  3. 服役加工硬化后高锰钢辙叉心轨应力/应变场分析%Numerical Analysis of Stress and Strain in a Service-work-hardened High Manganese Steel Crossi ng Nose

    Institute of Scientific and Technical Information of China (English)

    马鹏辉; 钱立和; 张汉林; 刘帅; 张福成

    2015-01-01

    本文建立由铁路辙叉和列车车轮组成的三维弹‐塑性有限元模型,研究高锰钢辙叉心轨的应力/应变场。文中考虑辙叉心轨在顶宽50 m m处的两种服役状态———服役前期未发生加工硬化和服役后期发生加工硬化,分析加工硬化对心轨应力/应变大小和分布的影响。对服役加工硬化的情况,考虑到距离工作表面不同深度处辙叉材料性能的不同,将心轨局部模型分层,并设置各层的材料性能;对未发生加工硬化的情况,为模型设置均匀的材料性能。结果表明,两种服役状态下辙叉心轨的von Mises应力和等效塑性应变均随深度的增加先快速增大,然后逐渐减小;与服役初期相比,服役后期心轨的最大等效应力增大约23%,最大等效塑性应变则降低约40%;塑性变形区域也明显减小,这是由于心轨在服役加工硬化后屈服强度已大幅提高。因此,在很大程度上,服役后期的加工硬化起着抑制心轨顶面塌陷和飞边形成的作用。此外,与未加工硬化心轨相比,加工硬化后心轨的最大等效应变与工作表面的距离由0.8 mm增大到了1.5 mm ,这表明易产生裂纹的位置有远离心轨表面的趋势。%A three dimensional elastic‐plastic finite element model consisting of a railway crossing and a train wheel was established to investigate the stress/strain field in a high manganese steel crossing nose . Two serv‐ice states of the crossing at a nose width of 50 mm were considered in the paper ,i.e.non‐work hardening cor‐responding to the early service stage and work hardening corresponding to the later service stage ,and the in‐fluence of service work hardening on the magnitude and distribution of stress/strain in the crossing nose was analyzed .In the case of simulation of service work hardening ,a layered model was constructed and various material properties were assigned to corresponding layers ,according to the effect of the distance from the sur‐face of an actual work‐hardened crossing nose on the material properties ,while a homogeneous material prop‐erty was assigned to the entire model with non‐work hardening . The results indicate that ,during both the ear‐ly and later service stages , the von Mises stress and equivalent plastic strain rapidly increase and then gradually decrease with the increase of the depth . As compared with the early service stage , during the later service stage ,the maximum von Mises stress of the crossing nose increased by about 23% ,while the maximum equiv‐alent plastic strain decreased by about 40% ,and the plastic strain zone reduced significantly . The reason for this is due to the largely increased yield strength of the crossing nose after service work hardening .It is there‐ fore believed that the surface quashing and the formation of overlap in the crossing nose may be largely sup‐pressed owing to the service work hardening . Furthermore , as compared to the non‐work‐hardened crossing nose , the distance between the location of the maximum equivalent plastic strain of the work‐hardened crossing nose and the surface of the crossing nose increases from 0.8 mm to 1.5 mm ,indicating that crack initiation site tends to move to locations farther away from the crossing nose surface .

  4. Gas chromatography/ion trap mass spectrometry applied for the analysis of triazine herbicides in environmental waters by an isotope dilution technique

    International Nuclear Information System (INIS)

    A gas chromatography/ion trap mass spectrometry method was developed for the analysis of simazine, atrazine, cyanazine, as well as the degradation products of atrazine, such as deethylatrazine and deisopropylatrazine in environmental water samples. Isotope dilution technique was applied for the quantitative analysis of atrazine in water at low ng/l levels. One liter of water sample spiked with stable isotope internal standard atrazine-d5 was extracted with a C18 solid-phase extraction cartridge. The analysis was performed on an ion trap mass spectrometer operated in MS/MS method. The extraction recoveries were in the range of 83-94% for the triazine herbicides in water at the concentrations of 24, 200, and 1000 ng/l, while poor recoveries were obtained for the degradation products of atrazine. The relative standard deviation (R.S.D.) were within the range of 3.2-16.1%. The detection limits of the method were between 0.75 and 12 ng/l when 1 l of water was analyzed. The method was successfully applied to analyze environmental water samples collected from a reservoir and a river in Hong Kong for atrazine detected at concentrations between 3.4 and 26 ng/l

  5. Performance Analysis of Gated Ring Oscillator Designed for Audio Frequency Range Asynchronous ADC

    Directory of Open Access Journals (Sweden)

    Anita Arvind Deshmukh

    2014-12-01

    Full Text Available This paper presents performance analysis of Gated R ing Oscillator (GRO. Proposed GRO is designed to employ in implementation of Time to Digital Convert er (TDC block of Asynchronous ADC. For an audio frequency range ADC, minimum GRO stages are designe d using asynchronous technique. So leads to reduced area and power. Compared to conventional Ri ng Oscillator (RO, we avoided to employ the gated clock; to evade clock design related problems like jitter, additional area and power. Instead we prefe rred gating of ring oscillator itself. Consequently duri ng sleep mode, GRO disables automatically which sav es the dynamic power. Furthermore it also provides fir st order noise shaping of the quantization and mismatch noise. Proposed GRO is implemented with 0. 18 μ m CMOS Digital Technology in Cadence Virtuso environment. GRO performance analysis shows oscillation frequency as 286 KHz with 327ps jitter and average power consumption of 1.08 μ W.

  6. Analysis of 5-hydroxymethylfurfural in foods by gas chromatography-mass spectrometry.

    Science.gov (United States)

    Teixidó, E; Santos, F J; Puignou, L; Galceran, M T

    2006-11-24

    A new, simple and selective method for the analysis of 5-hydroxymethylfurfural (HMF) in foods by gas chromatography coupled to mass spectrometry (GC-MS) is proposed. Several derivatising procedures based on the formation of an HMF silylated derivative using different reagents were studied. Among the derivatising reagents examined, N,O-bis-trimethylsilyltrifluoroacetamide (BSTFA) provided the best derivatisation yield. Sample clean-up was also optimised, using either liquid-liquid extraction with dichloromethane or solid-phase extraction (SPE) with several commercially available cartridges, and the best results were obtained using ENV+ cartridges. Quality parameters such as day-to-day and run-to-run precision (RSD<10%), linearity (between 25 and 700 ng g(-1)) and detection limit (6 ng g(-1)) were established. This method was successfully applied to the analysis of HMF content in several Spanish food samples from a local market, such as jam, honey, orange juice and bakery products. PMID:17010355

  7. Wastewater micropollutants as tracers of sewage contamination: analysis of combined sewer overflow and stream sediments.

    Science.gov (United States)

    Hajj-Mohamad, M; Aboulfadl, K; Darwano, H; Madoux-Humery, A-S; Guérineau, H; Sauvé, S; Prévost, M; Dorner, S

    2014-01-01

    A sensitive method was developed to measure the sediment concentration of 10 wastewater micropollutants selected as potential sanitary tracers of sewage contamination and include: nonsteroidal anti-inflammatory drugs (acetaminophen - ACE and diclofenac - DIC), an anti-epileptic drug (carbamazepine - CBZ), a β-blocker (atenolol - ATL), a stimulant (caffeine - CAF), a bronchodilator (theophylline - THEO), steroid hormones (progesterone - PRO and medroxyprogesterone - MedP), an artificial sweetener (aspartame - APM) and personal care products (N,N-diethyl-3-methylbenzamide - DEET). Natural sediments (combined sewer overflow and stream sediments) were extracted by ultrasonic-assisted extraction followed by solid-phase extraction. Analyses were performed using ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) using atmospheric pressure chemical ionisation in positive mode (APCI+) with a total analysis time of 4.5 min. Method detection limits were in the range of 0.01 to 15 ng g(-1) dry weight (dw) for the compounds of interest, with recoveries ranging from 75% to 156%. Matrix effects were observed for some compounds, never exceeding |±18%|. All results displayed a good degree of reproducibility and repeatability, with relative standard deviations (RSD) of less than 23% for all compounds. The method was applied to an investigation of stream and combined sewer overflow sediment samples that differed in organic carbon contents and particle size distributions. Acetaminophen, caffeine and theophylline (as confounded with paraxanthine) were ubiquitously detected at 0.13-22 ng g(-1) dw in stream bed sediment samples and 98-427 ng g(-1) dw in combined sewer overflow sediment samples. Atenolol (80.5 ng g(-1) dw) and carbamazepine (54 ng g(-1) dw) were quantified only in combined sewer overflow sediment samples. The highest concentrations were recorded for DEET (14 ng g(-1) dw) and progesterone (11.5 ng g(-1) dw) in stream bed and combined

  8. Preparation of Functional Magnetic Nanoparticles and its Application in Diagnostic Analysis

    Institute of Scientific and Technical Information of China (English)

    Qiong CHENG; Tu Zhi PENG; Ai Li LIU

    2005-01-01

    The magnetic nanoparticles modified with carboxyl functional group were synthesized and characterized. These nanoparticles covalently bound with hepatitis B surface antibody (HBsAb), were used to detect hepatitis B surface antigen (HBsAg) in immunovoltammetry. The detection limit was found to be 0.06 ng/mL, which is much higher than that of enzyme-linked immunosorbent assay (ELISA) used in clinical analysis.

  9. Multiple Equilibria and Interfirm Macro-Externality: An Analysis of Sluggish Real Adjustment

    OpenAIRE

    Yew-Kwang Ng; Ying Wu

    2004-01-01

    In an imperfectly competitive economy, a continuum of equilibria at the firm level exists under certain analytical conditions (Ng 1986). Extending the earlier analysis of a representative firm, this paper shows that even if the condition for a continuum of equilibria is not exactly satisfied, the factors of price-adjustment costs, interfirm heterogeneity, and macro-externality can cause the economy to be stuck at the quasi macroequilibria after aggregate demand experiences a contractionary sh...

  10. 3-Methyl-2-butene-1-thiol: identification, analysis, occurrence and sensory role of an uncommon thiol in wine.

    Science.gov (United States)

    San-Juan, Felipe; Cacho, Juan; Ferreira, Vicente; Escudero, Ana

    2012-09-15

    A highly uncommon odorant, 3-methyl-2-butene-1-thiol was detected by using Gas Chromatography-Olfactometry (GC-O) and unequivocally identified for the first time in wine. A purge and trap sampling technique which provides highly representative extracts for olfactometric analysis was used for the extraction of the volatile fraction of a Spanish red wine made from Prieto Picudo grapes. The identification of the odorant was achieved by multidimensional gas chromatography analysis of the same purge and trap extract. Mass spectrum and retention indices in both polar and non-polar columns allowed knowing unequivocally the identity. To obtain quantitative data a method was validated for the analysis of the compound at ng L(-1) level with acceptable precision. This powerful odorant presented an odor threshold in wine of 0.5-1 ng L(-1) and it has been detected in several Prieto Picudo wines at concentrations slightly above the odor threshold.

  11. Multi-element characterization of silicon nitride powders by instrumental and radiochemical neutron activation analysis

    Energy Technology Data Exchange (ETDEWEB)

    Franek, M.; Krivan, V. (Ulm Univ. (Germany). Sektion Analytik und Hoechstreinigung)

    1992-07-15

    An optimized instrumental neutron activation analysis method was applied to the comprehensive trace characterization of good- and high- purity silicon nitride powders of different origins. Experimental modes are given for 55 elements leading to limits of detection below 1 ng g[sup -] [sup 1] for 28 elements, between 1 and 100 ng g[sup -1] for 19 elements and higher than 100 ng g[sup -1] for 8 elements. For the removal of the radionuclides [sup 140]La, [sup 182]Ta and [sup 187]W, which cause the major activity in certain types of materials, radiochemical procedures based in cation exchange from 2 M HCl and anion exchange from 2 M HF were developed. [sup 64]Cu was selectively extracted with dithizone from 10 M HF for counting the 511-keV line. By radiochemical neutron activation analysis, the limits of detection were improved by up to three orders of magnitude. Comparison with results obtained by inductively coupled plasma (ICP) atomic emission spectrometry and ICP mass spectrometry shows satisfactory agreement and demonstrates the advantages of neutron activation analysis especially when low elements contents are to be determined. (author). 30 refs.; 2 figs.; 6 tabs.

  12. Cost-benefit analysis of using natural gas in vehicles of the Mexico City metropolitan area

    Energy Technology Data Exchange (ETDEWEB)

    Barrera-Roldan, A.; Trinidad-Garcia, H.G.; Diaz, L.; Perdomo, V.; Castillo, H.; Schifter, I. [Istituto Mexicano del Petroleo, Eje Central Lazaro Cardenas (Mexico)

    2000-07-01

    According to the Mexico City Metropolitan Area (MCMA) authorities the transport sector causes 75% of total pollutant emissions to the atmosphere, that is why to mitigate pollution several programs focus on reducing emissions from mobile sources, one of these programs promotes the use of Natural Gas (NG) in vehicles of the MCMA instead of gasoline or diesel. This work shows the cost-benefit analysis results of this program. The analysis considered the period 1999-2014. Projections for the MCMA vehicular fleet, and for the type and number of vehicles that would switch from gasoline or diesel to NG were made. To obtain the change in exhaust emissions, measurements were performed using the U. S. Federal Test Procedure 75 in vehicles consuming gasoline and NG Vehicle conversion, conversion shops, and gas station costs were considered in the analysis The estimated benefits stemmed from fuel price and efficiency differential, vehicle taxes, maintenance costs, and pollutant emission reductions. The Benefit/Cost ratio found was 3.79, and the vehicles suggested for conversion were those of intensive use circulating exclusively in the MCMA. Even though ozone formation simulations were done using the CIT trajectory model, the changes in ozone concentrations observed were smaller than the model resolution, mainly because the change in ozone precursors due to the fuel change in some of the MCMA vehicles, was less than 1% of total emissions. (authors)

  13. Removal of halogenated emerging contaminants from water by nitrogen-doped graphene decorated with palladium nanoparticles: Experimental investigation and theoretical analysis.

    Science.gov (United States)

    Li, Lei; Gong, Li; Wang, Yi-Xuan; Liu, Qi; Zhang, Jie; Mu, Yang; Yu, Han-Qing

    2016-07-01

    The removal performance and mechanisms of halogenated emerging contaminants from water by palladium decorated nitrogen-doped graphene (Pd/NG) were investigated in this study. For comparison, three catalysts of Pd/NG, palladium decorated graphene (Pd/G) and commercial Pd/C were initially explored to degrade tetrabromobisphenol A (TBBPA). After that, the influence of various environmental parameters on TBBPA removal by the Pd/NG catalyst was evaluated. Moreover, both Langmuir-Hinshelwood model and density functional theory (DFT) were adopted to theoretically elucidate the adsorption and the activation of TBBPA on the catalyst. The results show that the apparent rate constant of TBBPA dehalogenation was increased by 26.7% and 39.0% in the presence of the Pd/NG catalyst compared to the Pd/G and Pd/C ones. Higher temperature, catalyst dosage and alkaline conditions resulted in the enhancement of TBBPA dehalogenation by the Pd/NG catalyst, while humic acid in the solution had a negatively effect on the transformation of TBBPA. The corresponding rate constant value exhibited a 2.1- and 1.8-fold increase with the rise of temperature from 298 to 328 K and initial pH from 6.5 to 9.0, respectively. On the contrary, the rate constant was decreased by 78.9% in the presence of 15 mg L(-1) humic acid. Theoretical analysis revealed that both adsorption and activation processes of TBBPA on the Pd/NG catalyst were enhanced through the N doping into graphene framework. PMID:27107141

  14. A single laboratory-validated LC-MS method for the analysis of tulathromycin residues in bison and deer sera and selected tissues of white-tailed deer.

    Science.gov (United States)

    Boison, Joe O; Bachtold, Kali; Matus, Johanna; Alcorn, Jane; Woodbury, Murray

    2016-05-01

    The performance characteristics of a newly developed liquid chromatography-mass spectrometry (LC-MS) method were validated and demonstrated to be fit for purpose in a pharmacokinetic and tissue depletion study of white-tailed deer and bison. Tulathromycin was extracted from bison and deer sera with acetonitrile or trifluoroacetic acid and K2 HPO4 (pH 6.8) buffer solution and cleaned up on a conditioned Bond-Elut cartridge. Tulathromycin, retained on the cartridge; it was eluted with methanol containing 2% formic acid, dried, re-constituted in methanol/1% formic acid, and analyzed by LC-MS. The limit of quantification (LOQ) of the method was 0.6 ng/mL in serum and 0.6 ng/g in tissue with RSDs ≤ 10% and accurate over the linear calibration range of 0.8-100 ng/mL for bison serum, 0.6-50 ng/mL for deer serum, 100-2500 ng/g for deer muscle tissue, and 500-5000 ng/g for deer lung tissue, all with coefficients of determination, r(2) ≥0.99. The validated method was used to quantify the concentration of tulathromycin residues in serum of bison and deer and selected tissue (lung and muscle tissue) samples obtained from 10 healthy, white-tailed deer that were administered the therapeutic dose approved for cattle (i.e., a single 2.5 mg/kg subcutaneous injection of tulathromycin in the neck). The deer were included in a tulathromycin drug depletion study. © 2016 Her Majesty the Queen in Right of Canada. Drug Testing and Analysis © 2016 John Wiley & Sons, Ltd. PMID:27443215

  15. Removal of halogenated emerging contaminants from water by nitrogen-doped graphene decorated with palladium nanoparticles: Experimental investigation and theoretical analysis.

    Science.gov (United States)

    Li, Lei; Gong, Li; Wang, Yi-Xuan; Liu, Qi; Zhang, Jie; Mu, Yang; Yu, Han-Qing

    2016-07-01

    The removal performance and mechanisms of halogenated emerging contaminants from water by palladium decorated nitrogen-doped graphene (Pd/NG) were investigated in this study. For comparison, three catalysts of Pd/NG, palladium decorated graphene (Pd/G) and commercial Pd/C were initially explored to degrade tetrabromobisphenol A (TBBPA). After that, the influence of various environmental parameters on TBBPA removal by the Pd/NG catalyst was evaluated. Moreover, both Langmuir-Hinshelwood model and density functional theory (DFT) were adopted to theoretically elucidate the adsorption and the activation of TBBPA on the catalyst. The results show that the apparent rate constant of TBBPA dehalogenation was increased by 26.7% and 39.0% in the presence of the Pd/NG catalyst compared to the Pd/G and Pd/C ones. Higher temperature, catalyst dosage and alkaline conditions resulted in the enhancement of TBBPA dehalogenation by the Pd/NG catalyst, while humic acid in the solution had a negatively effect on the transformation of TBBPA. The corresponding rate constant value exhibited a 2.1- and 1.8-fold increase with the rise of temperature from 298 to 328 K and initial pH from 6.5 to 9.0, respectively. On the contrary, the rate constant was decreased by 78.9% in the presence of 15 mg L(-1) humic acid. Theoretical analysis revealed that both adsorption and activation processes of TBBPA on the Pd/NG catalyst were enhanced through the N doping into graphene framework.

  16. A novel miniaturized zinc oxide/hydroxylated multiwalled carbon nanotubes as a stir-brush microextractor device for carbamate pesticides analysis.

    Science.gov (United States)

    Makkliang, Fonthip; Kanatharana, Proespichaya; Thavarungkul, Panote; Thammakhet, Chongdee

    2016-04-21

    A novel miniaturized "stir-brush microextractor" was prepared using a zinc oxide/hydroxylated multiwalled carbon nanotubes (ZnO/MWCNTs-OH) coated stainless steel brush connected to a small dc motor. The synthesized zinc oxide on each strand of stainless steel had a flower-like nanostructure when observed by a scanning electron microscope (SEM). This structure produced a large surface area before it was coated with the hydroxylated multiwalled carbon nanotubes sorbent. Under optimal conditions, the developed device provided a good linearity for the extraction of carbofuran and carbaryl, in the range of 25-500 ng mL(-1) and 50-500 ng mL(-1), respectively, with low limits of detection of 17.5 ± 2.0 ng mL(-1) and 13.0 ± 1.8 ng mL(-1). It also provided a good stir-brush-to-stir-brush reproducibility (% relative standard deviation < 5.6%, n = 6). The device was applied for the extraction and preconcentration of carbamate pesticides in fruit and vegetable samples prior to analysis with a gas chromatograph coupled with a flame ionization detector (GC-FID). Carbofuran was found at 9.24 ± 0.93 ng g(-1) and carbaryl was detected at 7.05 ± 0.61 ng g(-1) with good recoveries in the range of 73.7 ± 10.0% to 108.4 ± 2.6% for carbofuran and 75.7 ± 10.0% to 111.7 ± 5.7% for carbaryl. PMID:27026597

  17. Analysis of major congeners of polybromobiphenyls and polybromodiphenyl ethers in office dust using high resolution gas chromatography-mass spectrometry.

    Science.gov (United States)

    Kefeni, Kebede K; Okonkwo, Jonathan O

    2012-05-01

    The study focused on analysis of polybromobiphenyls (PBBs) and polybromodiphenyl ethers (PBDEs) congeners in office dust obtained in Pretoria, South Africa. Of the 32 congeners considered for identification, (BB-1, 2, 4, 10, 15, 26, 29, 30, 31, 38, 49, 80, 103, 153, 155, 209 and BDE-3, 15, 17, 28, 47, 66, 77, 85, 99, 100, 126, 138, 153, 154, 183, 209) only BB-2, 4, 30, 153, 209 and BDE-47, 66, 85, 99, 153 and 209 congeners were detected. The sum of PBBs concentration detected in office dust ranged from ng g(-1) dry weight (dw) with a median and mean of 11.4 and 38.2 ng g(-1), respectively. On the other hand, the sum of PBDEs concentration detected ranged from 21.6 to 578.6 ng g(-1)dw with a median and mean of 162 and 169 ng g(-1)dw, respectively. A Spearman rank correlation between ∑(5)PBBs and ∑(6)PBDEs (r(s)=0.55, p=0.003), indicated a statistical significant positive correlation for the similarity of pollution sources for both compound classes. However, no correlation was observed between the number of electronic materials and summation of concentrations of PBBs and PBDEs congeners detected. Concentrations of PBDEs detected in this study are substantially lower than reported in office dust in developed countries.

  18. A multi-residue method for the analysis of pesticides and pesticide degradates in water using HLB solid-phase extraction and gas chromatography-ion trap mass spectrometry

    Science.gov (United States)

    Hladik, M.L.; Smalling, K.L.; Kuivila, K.M.

    2008-01-01

    A method was developed for the analysis of over 60 pesticides and degradates in water by HLB solid-phase extraction and gas-chromatography/mass spectrometry. Method recoveries and detection limits were determined using two surface waters with different dissolved organic carbon (DOC) concentrations. In the lower DOC water, recoveries and detection limits were 80%-108% and 1-12 ng/L, respectively. In the higher DOC water, the detection limits were slightly higher (1-15 ng/L). Additionally, surface water samples from four sites were analyzed and 14 pesticides were detected with concentrations ranging from 4 to 1,200 ng/L. ?? 2008 Springer Science+Business Media, LLC.

  19. Estacionalidad de egresos por neumonía en el Servicio de Pediatría del Hospital "Maximiliano Peralta" y su asociación con la migración indígena Ngöbe Buglé

    Directory of Open Access Journals (Sweden)

    María de la Paz Barboza- Argüello

    2005-04-01

    Full Text Available Objetivo: El presente estudio pretende determinar la magnitud del incremento en los casos de neumonía a finales de 2002, y la posible asociación de éste con la migración estacional de indígenas Ngöbe Buglé, a la zona de Los Santos, durante la época de cosecha de café. Metodología: Se analizaron los egresos por neumonía (Clasificación internacional de las CIE10= J18 del Servicio de Pediatría, del Hospital Max Peralta (HMP durante 1997-2003. Se revisaron los expedientes de los egresos del Servicio de Pediatría, por cuadros respiratorios (CIE10= J00-J99 referidos de la zona de Los Santos durante enero 2002-febrero 2003. Se estableció una definición de caso que identificara los pacientes indígenas con neumonía. Resultados: Durante el cuatrimestre octubre-enero de los años 1997 a 2002, el número de egresos por neumonía fue en promedio 1,7 veces mayor que el de los cuatrimestres febrero- mayo y junio-septiembre. En este cuatrimestre (octubre-enero, alrededor del 50% de los egresos correspondieron a pacientes referidos de los Santos. Durante enero 2002 y febrero 2003, los pacientes indígenas provenientes de la zona de Los Santos representaron el 40% (66/163 de todos los egresos por neumonía. La distribución de ellos se concentra (99% en los meses cuando ocurre la cosecha de café (noviembre-febrero. Conclusión: Existe un comportamiento estacional de los egresos por neumonía en el Servicio de Pediatría del HMP, durante 1997-2002. La estacionalidad es dada por población indígena migrante durante la época de café

  20. Mehmet Harmancı’nın Küçürek Öykülerindegündelik Kuşatma Altındaki İnsanın Varoluş Görüngüleri Existence Perspectives Of An Individual Under Daily Blockage In The Short Short Stories Of Mehmet Harmancı

    Directory of Open Access Journals (Sweden)

    Mutlu DEVECİ

    2013-03-01

    şme sürecinde değerlerini/ özünü koruyarak geleceğe taşınmalıdır. Diğerleri gibi kalmak, bireyleşememek, şey’ler dünyasında yitip gitmektir. İnsan gerçeğini tarihsel boyutuyla ele alan Mehmet Harmancı, öykülerinde, bireyin kendi oluşuna göndermede yapar. Anlam arayışı içerisindeki insanların; ölüm, aşk ve yalnızlık izlekleri etrafında eylem ve eylemsizliklerini dile getirir.Mehmet Harmancı Muhtemel Menkıbeler adlı eserinde yer alan küçürek öykülerinde, yaşamın anlamına yönelik sorgulamalarda bulunur. Türler arası geçişkenliğin dikkat çektiği bu eserde yazar, menkıbe türünün imkânlarını aşarak küçürek öykü türüne özgü bir anlatım yolu seçer. Öykülerde, şimdi’yi vareden değerler, geçmişin izdüşümünde kurulur ve gündelik kuşatma altındaki insanın varoluş görüngüleri sergilenir.Mehmet Harmancı, geleneğin izdüşümünde kurduğu Muhtemel Menkıbeler’inde moderniteyi ıskalamadan geleneği de modern olana ezdirmeden yol alır. Yazar, değerlerini yok sayarak medeniyet kurmaya çalışanlara karşı geleneği, modern dünyanın fenomenolojik temeli sayar. Bu açıdan modernite olarak kabul ettirilmeye çalışılan dünya/ yaşam yerine, temeli geleneğe dayalı bir modern dünya arzusu taşır. Muhtemel Menkıbeler’deki öykülerin pek çoğu böylesi bir ruh dünyasına aittir.Muhtemel Menkıbeler’deki öykülerde, geleneğin deneyimsel, kültürel ve nesneler belleğini başat değer olarak kabul eden yazar, yaşamın anlam arayışına yönelik sorgulamaları ile yol göstericilik özelliğini ön plana çıkarır.Çalışmada, Mehmet Harmancı’nın Muhtemel Menkıbeler adlı eserinde yer alan öyküleri analiz edilirken, menkıbe ile küçürek öykü türleri arasındaki ilişkiye dikkat çekilerek; gelenek ile modernite arasındaki insan görüngülerine yer verildi.

  1. Analysis of selective androgen receptor modulators by gas chromatography-microchip atmospheric pressure photoionization-mass spectrometry.

    Science.gov (United States)

    Luosujärvi, Laura; Haapala, Markus; Thevis, Mario; Saarela, Ville; Franssila, Sami; Ketola, Raimo A; Kostiainen, Risto; Kotiaho, Tapio

    2010-02-01

    A gas chromatography-microchip atmospheric pressure photoionization-mass spectrometric (GC-microAPPI-MS) method was developed and used for the analysis of three 2-quinolinone-derived selective androgen receptor modulators (SARMs). SARMs were analyzed from spiked urine samples, which were hydrolyzed and derivatized with N-methyl-N-(trimethylsilyl)trifluoroacetamide before analysis. Trimethylsilyl derivatives of SARMs formed both radical cations (M(+*)) and protonated molecules ([M + H](+)) in photoionization. Better signal-to-noise ratios (S/N) were obtained in MS/MS analysis using the M(+*) ions as precursor ions than using the [M + H](+) ions, and therefore the M(+*) ions were selected for the precursor ions in selected reaction monitoring (SRM) analysis. Limits of detection (LODs) with the method ranged from 0.01 to 1 ng/mL, which correspond to instrumental LODs of 0.2-20 pg. Limits of quantitation ranged from 0.03 to 3 ng/mL. The mass spectrometric response to the analytes was linear (R > or = 0.995) from the LOQ concentration level up to 100 ng/mL concentration, and intra-day repeatabilities were 5%-9%. In addition to the GC-microAPPI-MS study, the proof-of-principle of gas chromatography-microchip atmospheric pressure chemical ionization-Orbitrap MS (GC-microAPCI-Orbitrap MS) was demonstrated.

  2. Analysis of 3-chloro-4-(dichloromethyl)-5-hydroxy-2(5H)-furanone (MX) and its brominated analogues in chlorine-treated water by gas chromatography coupled to triple quadrupole tandem mass spectrometry (GC-QqQ-MS/MS).

    Science.gov (United States)

    Planas, Carles; Ventura, Francesc; Caixach, Josep; Martín, Jordi; Boleda, M Rosa; Paraira, Miquel

    2015-11-01

    A simple, selective and sensitive method for the analysis of the strong mutagen 3-chloro-4-(dichloromethyl)-5-hydroxy-2(5H)-furanone (MX) and its brominated analogues (BMXs) in chlorine-treated water has been developed. The method is based on gas chromatography coupled to triple quadrupole tandem mass spectrometry (GC-QqQ-MS/MS), previous liquid-liquid extraction (LLE) of a smaller sample volume compared to other methods and on-line derivatization with a silylation reactive. GC-QqQ-MS/MS has been raised as an alternative easier to perform than gas chromatography coupled to high resolution mass spectrometry (GC-HRMS) for the analysis of MX and BMXs, and it allows to achieve low LODs (0.3 ng/L for MX and 0.4-0.9 ng/L for BMXs). This technique had not been previously described for the analysis of MX and BMXs. Quality parameters were calculated and real samples related to 3 drinking water treatment plants (DWTPs), tap water and both untreated and chlorinated groundwater were analyzed. Concentrations of 0.3-6.6 ng/L for MX and 1.0-7.3 ng/L for BMXs were detected. Results were discussed according to five of the main factors affecting MX and BMXs formation in chlorine-treated water (organic precursors, influence of bromide ions, evolution of MX and BMXs in the drinking water distribution system, groundwater chlorination and infiltration of water coming from chlorination processes in groundwater).

  3. Analysis and simulation of creativity learning by means of artificial neural networks.

    Science.gov (United States)

    Memmert, Daniel; Perl, Jürgen

    2009-04-01

    The paper presents a new neural network approach for analysis and simulation of creative behavior. The used concept of Dynamically Controlled Neural Gas (DyCoNG) entails a combination of Dynamically Controlled Network [Perl, J. (2004a). A neural network approach to movement pattern analysis. Human Movement Science,23, 605-620] and Growing Neural Gas (Fritzke, 1995) by quality neurons. A quality neuron reflects the rareness of a piece of information and therefore can measure the originality of a recorded activity that was assigned to the neuron during the network training. The DyCoNG approach was validated using data from a longitudinal field-based study. The creative behavior of 42 participants in standardized test situations was tested in a creative training program lasting six months. The results from the DyCoNG-based simulation show that the network is able to separate main process types and reproduce recorded creative learning processes by means of simulation. The results are discussed in connection with practical implications in team sports and with a view to future investigations. PMID:19110331

  4. Environmental monitoring for uranium and neptunium at Yucca Mountain using Epithermal Neutron Activation Analysis

    International Nuclear Information System (INIS)

    Epithermal Neutron Activation Analysis (ENAA) is investigated as an analysis method for uranium and neptunium in environmental samples from Yucca Mountain. The design and construction of a facility for this technique are described. Theoretical improvement in sensitivity for ENAA over thermal NAA (TNAA) is discussed and compared to experimental results for different sample types. Uranium is analyzed in eight different sample matrices, including samples from Yucca Mountain. Neptunium has been studied only in AGV-1 Granite. As predicted by theory, uranium shows a high experimental sensitivity improvement factor (average = 7.76), while neptunium has a factor of only 0.49. Detection limits for uranium using ENAA range from 6 to 52 ppb by weight (2.6 to 17 ng in sample) for the different matrices. Neptunium shows a detection limit of 57 ppb by weight (6.2 ng in sample) in AGV-1 Granite using ENAA. Using TNAA, neptunium can be analyzed to 35 ppB by weight (3.4 ng in sample)

  5. Integrating Paper Chromatography with Electrochemical Detection for the Trace Analysis of TNT in Soil

    Directory of Open Access Journals (Sweden)

    Patrick Ryan

    2015-07-01

    Full Text Available We report on the development of an electrochemical probe for the trace analysis of 2,4,6-trinitrotoluene (TNT in soil samples. The probe is a combination of graphite electrodes, filter paper, with ethylene glycol and choline chloride as the solvent/electrolyte. Square wave chromatovoltammograms show the probes have a sensitivity for TNT of 0.75 nA/ng and a limit of detection of 100 ng. In addition, by taking advantage of the inherent paper chromatography step, TNT can be separated in both time and cathodic peak potential from 4-amino-dinitrotolene co-spotted on the probe or in soil samples with the presence of methyl parathion as a possible interferent.

  6. Trace analysis of multi-class pesticide residues in Chinese medicinal health wines using gas chromatography with electron capture detection

    Science.gov (United States)

    Kong, Wei-Jun; Liu, Qiu-Tao; Kong, Dan-Dan; Liu, Qian-Zhen; Ma, Xin-Ping; Yang, Mei-Hua

    2016-02-01

    A method is described for multi-residue, high-throughput determination of trace levels of 22 organochlorine pesticides (OCPs) and 5 pyrethroid pesticides (PYPs) in Chinese medicinal (CM) health wines using a QuEChERS (quick, easy, cheap, effective, rugged, and safe) based extraction method and gas chromatography-electron capture detection (GC-ECD). Several parameters were optimized to improve preparation and separation time while still maintaining high sensitivity. Validation tests of spiked samples showed good linearities for 27 pesticides (R = 0.9909-0.9996) over wide concentration ranges. Limits of detection (LODs) and quantification (LOQs) were measured at ng/L levels, 0.06-2 ng/L and 0.2-6 ng/L for OCPs and 0.02-3 ng/L and 0.06-7 ng/L for PYPs, respectively. Inter- and intra-day precision tests showed variations of 0.65-9.89% for OCPs and 0.98-13.99% for PYPs, respectively. Average recoveries were in the range of 47.74-120.31%, with relative standard deviations below 20%. The developed method was then applied to analyze 80 CM wine samples. Beta-BHC (Benzene hexachloride) was the most frequently detected pesticide at concentration levels of 5.67-31.55 mg/L, followed by delta-BHC, trans-chlordane, gamma-BHC, and alpha-BHC. The validated method is simple and economical, with adequate sensitivity for trace levels of multi-class pesticides. It could be adopted by laboratories for this and other types of complex matrices analysis.

  7. Analysis on sensitivity of Neisseria gonorrhoea to cephalosporin antibiotics and multi-antigen sequence typing%淋病奈瑟球菌对头孢菌素类药物的敏感性及多抗原序列分型研究

    Institute of Scientific and Technical Information of China (English)

    蓝银苑; 吴兴中; 覃晓琳; 黄进梅; 薛耀华; 曾维英; 欧江丽; 唐三梅; 方铭恒

    2015-01-01

    Objective To understand the molecular types of clinical isolated Neisseria gonorrhoeae (NG) in Guangzhou, and to analyze the sensitivity to the cephalosporin antibiotics. Methods A total of 121 NG strains isolated from Guangzhou Skin Disease Prevention and Control Center were included in this study. The agar dilution method was used to determine the minimum inhibitory concentrations (MIC) to ceftriaxone and cefixime. DNA was extracted for NG multi-antigen sequence typing (NG-MAST). Results The drug-resistance analysis showed that none of the strains appeared to be resistant to ceftriaxone and cefixime. However, the low sensitivity rates were 13.2%(16/121) and 2.5%(3/121), respectively. A total of 82 genotypes were identified among the 121 isolates. The most prevalent genotype was ST1768 (n=6) in NG strains isolated, followed by ST2083 (n=4), ST5062 (n=4), ST10229 (n=4), ST1766 (n=3), ST1866 (n=3), and ST10339 (n=3). There were 12 genotypes containing 2 NG strains, and 63 genotypes [76.8%(63/121) ] containing only one NG strain. Among the 13 NG strains with low sensitivity to ceftriaxone , there were 2 strains of ST10339 and 2 strains of ST10242 , and the rest were single genotype. Conclusions The 121 NG strains have relatively high sensitivity to ceftriaxone and cefixime. The genotypes of these NG-MAST strains are high diversity.%目的 了解广州市临床分离淋病奈瑟球菌 (NG) 的分子型别及其对头孢菌素类抗生素的敏感性. 方法 收集广东省皮肤性病防治中心临床分离的121株NG,用琼脂稀释法测定菌株对头孢曲松和头孢克肟的最小抑菌浓度(MIC),并进行NG多抗原测序分型(NG-MAST). 结果 未发现NG对头孢曲松和头孢克肟耐药的菌株,但它们的低敏率分别达到13.2%(16/121)和2.5%(3/121). 121株NG共有82个型别,其中ST1768共6株,ST2083、ST5062和ST10229各4株,ST1766、ST1866和ST10339各3株, 有12个ST族群包含2株菌株,其余63 个型别仅含1 株菌株,占76.8%(63/121).

  8. Broad spectrum analysis of polar and apolar organic compounds in submicron atmospheric particles.

    Science.gov (United States)

    Fontal, Marta; van Drooge, Barend L; López, Jordi F; Fernández, Pilar; Grimalt, Joan O

    2015-07-24

    A method for the quantitative analysis of organic compounds on submicron particulate matter (PM1) collected on quartz filters was developed. The compounds analyzed encompassed C22-C35 alkanes, polycyclic aromatic hydrocarbons (PAHs), quinones, levoglucosan, cis-pinonic acid and short chain dicarboxylic acids such as malonic, succinic, glutaric, adipic, suberic, azelaic, malic and phthalic acids. The method included extraction with a pressure liquid extraction system, sample filtration though glass fibre filter, fractionation by high performance liquid chromatography and subsequent analysis by gas chromatography coupled to mass spectrometry. The study of the extraction efficiency of different solvent mixtures showed that DCM:MeOH 1:1 was the one providing the highest recoveries for all compounds. Extraction temperatures of 100°C provided better results than 60°C or 80°C. This method provided comparable extraction efficiency and qualitative and quantitative data to those involving Soxhlet extraction. Method recoveries for alkanes, most PAH, quinones and polar compounds calculated from spiked real samples were 52-72%, 78-101%, 50-62% and 76-104%, respectively, reproducibilities were 2-28%, 7-29%, 10-27% and 5-28%, respectively, limits of quantification were 0.01-0.1ng/m(3), 0.01-0.27ng/m(3), 0.04ng/m(3) and 0.32-2.8ng/m(3), respectively, which affords the quantification of a broad number of primary and secondary organic constituents of submicron aerosols. PMID:26054559

  9. [Analysis of phthalates in plastic food-packaging bags by thin layer chromatography].

    Science.gov (United States)

    Chen, Hui; Wang, Yuan; Zhu, Ruohua

    2006-01-01

    The method for simultaneous determination of four phthalates, namely dimethyl phthalate (DMP), diethyl phthalate (DEP), di-n-butyl phthalate (DBP) and di (2-ethylhexyl) phthalate (DEHP) in plastic food-packaging bags by thin layer chromatography (TLC) was developed. The plastic food-packaging bags were extracted with ethanol by ultrasonication, then the mixture was filtrated through membrane (0.45 microm). The mixture of ethyl acetate-anhydrous ether-isooctane (1 : 4 : 15, v/v) was used as developing agent on the TLC silica gel plate for development. The filtered liquid was spotted on the TLC plate dealt by acetone, and detected with scanning wavelength of 275 nm and reference wavelength of 340 nm. The qualitative analysis of the phthalates was performed using the R(f) values of the chromatogram. The quantitative analysis was performed with external standard method. Good linearities were obtained for DMP, DEP, DBP and DEHP. The detection limits were 2.1 ng for DMP, 2.4 ng for DEP, 3.4 ng for DBP and 4.0 ng for DEHP. The relative standard deviations (RSDs) of the four phthalates were 2.8% - 3.5%. The recoveries of the four phthalate standards in real sample were 78.58% - 111.04%. The method presented has the advantages of high precision, high sensitivity, small sample size, and simple pretreatment . The method was used to detect the four phthalates in the food-packaging bags. The contents in real samples were close to the results by gas chromatography.

  10. Development of a non-chromatographic method for the speciation analysis of inorganic antimony in mushroom samples by hydride generation atomic fluorescence spectrometry

    Science.gov (United States)

    Sousa Ferreira, Hadla; Costa Ferreira, Sergio Luis; Cervera, M. Luisa; de la Guardia, Miguel

    2009-06-01

    A simple and sensitive method has been developed for the direct determination of toxic species of antimony in mushroom samples by hydride generation atomic fluorescence spectrometry (HG AFS). The determination of Sb(III) and Sb(V) was based on the efficiency of hydride generation employing NaBH 4, with and without a previous KI reduction, using proportional equations corresponding to the two different measurement conditions. The extraction efficiency of total antimony and the stability of Sb(III) and Sb(V) in different extraction media (nitric, sulfuric, hydrochloric, acetic acid, methanol and ethanol) were evaluated. Results demonstrated that, based on the extraction yield and the stability of extracts, 0.5 mol L - 1 H 2SO 4 proved to be the best extracting solution for the speciation analysis of antimony in mushroom samples. The limits of detection of the developed methodology were 0.6 and 1.1 ng g - 1 for Sb(III) and Sb(V), respectively. The relative standard derivation was 3.8% (14.7 ng g - 1 ) for Sb(V) and 5.1% (4.6 ng g - 1 ) for Sb(III). The recovery values obtained for Sb(III) and Sb(V) varied from 94 to 106% and from 98 to 105%, respectively. The method has been applied to determine Sb(III), Sb(V) and total Sb in five different mushroom samples; the Sb(III) content varied from 4.6 to 11.4 ng g - 1 and Sb(V) from 14.7 to 21.2 ng g - 1 . The accuracy of the method was confirmed by the analysis of a certified reference material of tomato leaves.

  11. A new gas chromatography/mass spectrometry method for the simultaneous analysis of target and non-target organic contaminants in waters.

    Science.gov (United States)

    Gómez, M J; Gómez-Ramos, M M; Agüera, A; Mezcua, M; Herrera, S; Fernández-Alba, A R

    2009-05-01

    In this study we developed a GC-MS method for the analysis of priority pollutants, personal care products (PCPs) and other emerging contaminants in waters using large volume injection with backflushing. Analyses are performed in the SIM/scan mode, so that in addition to the targeted organic contaminants, this method allows the simultaneous screening of non-target compounds. The scan data are analysed using Deconvolution Reporting Software (DRS) which screens the results for 934 organic contaminants. Deconvolution helps identify contaminants that are buried in the chromatogram by co-extracted materials and significantly reduces chromatographic resolution requirements, allowing shorter analysis times. All compounds have locked retention times and we can continually update and extend the mass spectral library including new compounds. Linearity and limits of detection in SIM and full-scan mode were studied. Method detection limits (MDLs) in effluent wastewater ranged in most of the cases from 1 to 36 ng/L in SIM mode and from 4 to 66 ng/L in full-scan mode; while in river water from 0.4 to 14 and 2-29 ng/L in SIM and full-scan mode, respectively. We obtained a linearity of the calibration curves over two orders of magnitude. The method has been applied to the screening of a large number of organic contaminants--not only to a subset of targets--in urban wastewaters from different wastewater treatment plants and also in river waters. Most of the target compounds were detected at concentration levels ranging from 11 to 8697 ng/L and from 7 to 1861 ng/L in effluent wastewater and river waters, respectively. Additionally, a group of 12 new compounds were automatically identified using the AMDIS and NIST libraries. Other compounds, such as the 4-amino musk xylene, a synthetic fragrance metabolite, which was not included in the databases, but has been manually searched in the full-scan chromatograms.

  12. Densitometric HPTLC analysis of 8-gingerol in Zingiber officinale extract and ginger-containing dietary supplements, teas and commercial creams

    Institute of Scientific and Technical Information of China (English)

    Prawez Alam

    2013-01-01

    Objective: To develop and validate a simple, accurate HPTLC method for the analysis of 8-gingerol and to determine the quantity of 8-gingerol in Zingiber officinale extract and ginger-containing dietary supplements, teas and commercial creams. Methods:The analysis was performed on 10×20 cm aluminium-backed plates coated with 0.2 mm layers of silica gel 60 F254 (E-Merck, Germany) with n-hexane: ethyl acetate 60: 40 (v/v) as mobile phase. Camag TLC Scanner III was used for the UV densitometric scanning at 569. Results:This system was found to give a compact spot of 8-gingerol at retention factor (Rf) value of (0.39±0.04) and linearity was found in the ranges 50-500 ng/spot (r2=0.9987). Limit of detection (12.76 ng/spot), limit of quantification (26.32 ng/spot), accuracy (less than 2 %) and recovery (ranging from 98.22-99.20) were found satisfactory. Conclusions:The HPTLC method developed for quantification of 8-gingerol was found to be simple, accurate, reproducible, sensitive and is applicable to the analysis of 8-gingerol in Zingiber officinale extract and ginger-containing dietary supplements, teas and commercial creams.

  13. Solid-phase extraction in combination with dispersive liquid-liquid microextraction and ultra-high performance liquid chromatography-tandem mass spectrometry analysis: the ultra-trace determination of 10 antibiotics in water samples.

    Science.gov (United States)

    Liang, Ning; Huang, Peiting; Hou, Xiaohong; Li, Zhen; Tao, Lei; Zhao, Longshan

    2016-02-01

    A novel method, solid-phase extraction combined with dispersive liquid-liquid microextraction (SPE-DLLME), was developed for ultra-preconcentration of 10 antibiotics in different environmental water samples prior to ultra-high performance liquid chromatography-tandem mass spectrometry detection. The optimized results were obtained as follows: after being adjusted to pH 4.0, the water sample was firstly passed through PEP-2 column at 10 mL min(-1), and then methanol was used to elute the target analytes for the following steps. Dichloromethane was selected as extraction solvent, and methanol/acetonitrile (1:1, v/v) as dispersive solvent. Under optimal conditions, the calibration curves were linear in the range of 1-1000 ng mL(-1) (sulfamethoxazole, cefuroxime axetil), 5-1000 ng mL(-1) (tinidazole), 10-1000 ng mL(-1) (chloramphenicol), 2-1000 ng mL(-1) (levofloxacin oxytetracycline, doxycycline, tetracycline, and ciprofloxacin) and 1-400 ng mL(-1) (sulfadiazine) with a good precision. The LOD and LOQ of the method were at very low levels, below 1.67 and 5.57 ng mL(-1), respectively. The relative recoveries of the target analytes were in the range from 64.16% to 99.80% with relative standard deviations between 0.7 and 8.4%. The matrix effect of this method showed a great decrease compared with solid-phase extraction and a significant value of enrichment factor (EF) compared with dispersive liquid-liquid microextraction. The developed method was successfully applied to the extraction and analysis of antibiotics in different water samples with satisfactory results. PMID:26780712

  14. Analysis of the injection process in direct injected natural gas engines. Part 1: Study of unconfined and in-cylinder plume behavior

    Energy Technology Data Exchange (ETDEWEB)

    Jennings, M.J.; Jeske, F.R. (Ricardo North America, Burr Ridge, IL (United States))

    1994-10-01

    A study of natural gas (NG) direct injection (DI) processes has been performed using multidimensional computational fluid dynamics analysis. The purpose was to improve the understanding of mixing in DI NG engines. Calculations of injection into a constant-volume chamber were performed to document unconfined plume behavior. A full three-dimensional calculation of injection into a medium heavy-duty diesel engine cylinder was also performed to study plume behavior in engine geometries. The structure of the NG plume is characterized by a core of unmixed fuel confined to the near-field of the jet. This core contains the bulk of the unmixed fuel and is mixed by the turbulence generated by the jet shear layer. The NG plume development in the engine is dominated by combustion chamber surface interactions. A Coanda effect causes plume attachment to the cylinder head, which has a detrimental impact on mixing. Unconfined plume calculations with different nozzle hole sizes demonstrate that smaller nozzle holes are more effective at mixing the fuel and air.

  15. Trace analysis of trimethoprim and sulfonamide, macrolide, quinolone, and tetracycline antibiotics in chlorinated drinking water using liquid chromatography electrospray tandem mass spectrometry

    Science.gov (United States)

    Ye, Z.; Weinberg, H.S.; Meyer, M.T.

    2007-01-01

    A multirun analytical method has been developed and validated for trace determination of 24 antibiotics including 7 sulfonamides, 3 macrolides, 7 quinolones, 6 tetracyclines, and trimethoprim in chlorine-disinfected drinking water using a single solid-phase extraction method coupled to liquid chromatography with positive electrospray tandem mass spectrometry detection. The analytes were extracted by a hydrophilic-lipophilic balanced resin and eluted with acidified methanol (0.1% formic acid), resulting in analyte recoveries generally above 90%. The limits of quantitation were mostly below 10 ng/L in drinking water. Since the concentrated sample matrix typically caused ion suppression during electrospray ionization, the method of standard addition was used for quantitation. Chlorine residuals in drinking water can react with some antibiotics, but ascorbic acid was found to be an effective chlorine quenching agent without affecting the analysis and stability of the antibiotics in water. A preliminary occurrence study using this method revealed the presence of some antibiotics in drinking waters, including sulfamethoxazole (3.0-3.4 ng/L), macrolides (1.4-4.9 ng/L), and quinolones (1.2-4.0 ng/L). ?? 2007 American Chemical Society.

  16. Effect of six Chinese spices on heterocyclic amine profiles in roast beef patties by ultra performance liquid chromatography-tandem mass spectrometry and principal component analysis.

    Science.gov (United States)

    Zeng, Maomao; He, Zhiyong; Zheng, Zongping; Qin, Fang; Tao, Guanjun; Zhang, Shuang; Gao, Yahui; Chen, Jie

    2014-10-01

    The effects of Chinese spices on the profiles of 17 heterocyclic amines (HAs) from seven HA categories were investigated in roast beef patties using Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry (UPLC-MS/MS) and principal component analysis. Three groups of HAs, imidazopyridines (PhIP, DMIP, and 1,5,6-TMIP), imidazoquinoxalines (MeIQx and 4,8-DiMeIQx), and β-carbolines (harman and norharman), were detected and quantified in all of the samples. The results demonstrated that the total HA and imidazopyridine profiles could clearly be affected by 1% pricklyash peel (14.1 ± 0.76 and 6.06 ± 0.32 ng/g), chilli (41.0 ± 0.01 and 23.0 ± 0.52 ng/g), and cumin (59.9 ± 2.44 and 31.1 ± 3.06 ng/g), in comparison with control values of 21.8 ± 2.40 and 14.3 ± 2.04 ng/g, respectively. The difference was only significant (p spices in meat processing to minimize HA formation.

  17. Molecularly imprinted polymer cartridges coupled on-line with high performance liquid chromatography for simple and rapid analysis of human insulin in plasma and pharmaceutical formulations.

    Science.gov (United States)

    Moein, Mohammad Mahdi; Javanbakht, Mehran; Akbari-adergani, Behrouz

    2014-04-01

    In this paper, a novel method is described for automated determination of human insulin in biological fluids using principle of sequential injection on a molecularly imprinted solid-phase extraction (MISPE) cartridge as a sample clean-up technique combined with high performance liquid chromatography (HPLC). The water-compatible molecularly imprinted polymers (MIPs) were prepared using methacrylic acid as a functional monomer, ethylene glycol dimethacrylate as a cross-linker, chloroform as a porogen and insulin as a template molecule. The imprinted polymers were then employed as the solid-phase extraction sorbent for on-line extraction of insulin from human plasma samples. To achieve the best condition, influential parameters on the extraction efficiency were thoroughly investigated. Rapid and simple analysis of the hormone was successfully accomplished through the good selectivity of the prepared sorbent coupled with HPLC. Limits of detection (LOD) and quantification (LOQ) of 0.2 ng mL(-1), 0.7 ng mL(-1), and 0.03 ng mL(-1), 0.1 ng mL(-1) were obtained in plasma and urine respectively. The obtained data exhibited the great recoveries for extraction of insulin from human plasma and pharmaceutical samples, higher than 87%. PMID:24607106

  18. Automated liquid chromatography-tandem mass spectrometry method for the analysis of firocoxib in urine and plasma from horse and dog.

    Science.gov (United States)

    Letendre, Laura; Kvaternick, Valerie; Tecle, Berhane; Fischer, James

    2007-06-15

    A rugged, sensitive and efficient liquid chromatography-tandem mass spectrometry method was developed and validated for the quantitative analysis of firocoxib in urine from 5 to 3000 ng/mL and in plasma from 1 to 3000 ng/mL. The method requires 200 microL of either plasma or urine and includes sample preparation in 96-well solid phase extraction (SPE) plates using a BIOMEK 2000 Laboratory Automated Workstation. Chromatographic separation of firocoxib from matrix interferences was achieved using isocratic reversed phase chromatography on a PHENOMENEX LUNA Phenyl-Hexyl column. The mobile phase was 45% acetonitrile and 55% of a 2 mM ammonium formate buffer. The method was accurate (88-107%) and precise (CV93% were achieved and ionization efficiencies (due to matrix effects) were >72%. Extensive stability and ruggedness testing was also performed; therefore, the method can be used for pharmacokinetic studies as well as drug monitoring and screening. The data presented here is the first LC-MS/MS method for the quantitation of firocoxib in plasma (LLOQ of 1 ng/mL), a 25-fold improvement in sensitivity over the HPLC-UV method and the first quantitative method for firocoxib in urine (LLOQ of 5 ng/mL). Additionally the sample preparation process has been automated to improve efficiency. PMID:17459786

  19. STUDY ON THE ATTACK CONTROL ACTION OF ELECTRO-ACUPUNCTURE ON T(A)IY(A)NG(太阳 EX-HN5)FOR MIGRAINE DUE TO HYPERACTIVITY OF LIVER YANG:RANDOMIED CONTROLED TRIAL%电针太阳穴治疗偏头痛肝阳上亢证的控制发作效应研究

    Institute of Scientific and Technical Information of China (English)

    周建伟; 李季; 李宁; 张凡; 胡玲香; 赵菁菁; 张颜; 王成伟

    2008-01-01

    Objective To discuss the difference of electro-acupuncture and drug in controlling the attack of migraine due to hyperactivity of liver yang.Methods Three-centered random control method was used,and 300 qualified cases were randomly divided into treatment group(146 cases according to the design)and control group(140 cases),which were respectively treated with eIectro-acupuncture and drugs(Compound Aminopyrine Phenacetin Tablets,Ergotamine Caffeine Tablets,Diazepam Tablets),and observed the overall effect and scores of headache,accompanying symptoms,psychological and social adaptability scores,life quality scores,TCM symptoms scores and follow-up results before and after the treatment.Results In treatment group,the successful rate of attack control was 47.3%,the improvement rate was 73.3%,and the total effectiveness was 90.4%,the clinical control rate and improvement were much superior to control group(the clinical controI rate 35.7%,improvement 61.4%,the totaI effectiveness 85.7%),P<0.01,the difference in effect was mainly reflected in patients with moderate severity;the total scores of TCM syndrome after the treatment was obviously significant or very obviously significant(P<0.05,P<0.01),but the headache scores between two groups was not obviously significant(P>0.05),the difference in accompanying symptoms was significant(P<0.01);the long term attack control action in two groups was not satisfactory,the recurrence was similar(P>0.05),the severity of headache in recurrence cases of treatment group was alleviated and superior to control group(P<0.01),the occurrence of headache after the treatment is much less than that before the treatment and the situation after 2 months was superior to controI group(P<0.01).Sleepiness and redness of face in some patients which were seen in control group weren't seen in treatment group.Conclusion Electro-acupuncture on Tàiyáng(太阳EX-HN5)can control the attack of migraine due to hyperactivity of liver yang

  20. Simultaneous determination of minerals in different varieties of forage by neutron activation analysis; Analise por ativacao com neutrons aplicada para a determinacao simultanea de minerais em diferentes especies de forrageira

    Energy Technology Data Exchange (ETDEWEB)

    Armelin, Maria Jose A.; Piasentin, Ricardo M.; Souza, Marcos P. de [Instituto de Pesquisas Energeticas e Nucleares (IPEN), Sao Paulo, SP (Brazil). Supervisao de Radioquimica; Cruvinel, Paulo E. [Empresa Brasileira de Pesquisa Agropecuaria, Sao Carlos, SP (Brazil). Centro Nacional de Pesquisa e Desenvolvimento de Instrumentacao Agropecuaria

    1996-07-01

    Six varieties of forages were analyzed by instrumental neutron activation analysis. The experimental conditions allowed the determination of Ca(5); K(%); Mg ({mu}g/g); Mn({mu}g/g); Ti({mu}g/g) and V(ng/g) in 30 minutes, approximately. The accuracy of the method was evaluated by means of reference material analysis. The precision of the results was lower than 10%, except for Ti and some cases for Na was near 20%. (author)

  1. Chemical analysis of fish bile extracts for monitoring endocrine disrupting chemical exposure in water: Bisphenol A, alkylphenols, and norethindrone.

    Science.gov (United States)

    Wu, Minghong; Pan, Chenyuan; Yang, Ming; Xu, Bentuo; Lei, Xiangjie; Ma, Jing; Cai, Ling; Chen, Jingsi

    2016-01-01

    The present study determined concentrations of estrogenic bisphenol A (BPA), nonylphenol, octylphenol (4-tert-octylphenol), butylphenol (4-tert-butylphenol), and progestogenic norethindrone by liquid chromatography-tandem mass spectrometry in bile extracts from field fish from the Xin'an River and market fish in Shanghai, China. Compared with the field fish, endocrine disrupting chemical (EDC) concentrations in market fish bile were at relatively high levels with high detectable rates. The average concentrations of BPA, nonylphenol, 4-tert-octylphenol, 4-tert-butylphenol, and norethindrone in field fish bile were 30.1 µg/L, 203 µg/L, 4.69 µg/L, 7.84 µg/L, and 0.514 µg/L, respectively; in market fish bile they were 240 µg/L, 528 µg/L, 76.5 µg/L, 12.8 µg/L, and 5.26 µg/L, respectively; and in the surface water of Xin'an River they were 38.8 ng/L, 7.91 ng/L, 1.98 ng/L, 2.66 ng/L, and 0.116 ng/L, respectively. The average of total estrogenic activity of river water was 3.32 ng/L estradiol equivalents. High bioconcentration factors (BCFs) were discovered for all 5 EDCs (≧998-fold) in field fish bile. Furthermore, the authors analyzed the BCF value of BPA in fish bile after 30-d exposure to environmentally relevant concentrations of BPA in the laboratory, and the analysis revealed that BCF in fish bile (BCF(Fish bile)) changed in an inverse concentration-dependent manner based on the log10-transformed BPA concentration in water. Strikingly, the data from the field study were well fitted within this trend. The data together suggested that analysis of fish bile extracts could be an efficient method for assessing waterborne EDCs exposure for aquatic biota.

  2. Rhamnolipid biosurfactant analysis using online turbulent flow chromatography-liquid chromatography-tandem mass spectrometry.

    Science.gov (United States)

    Behrens, Beate; Helmer, Patrick O; Tiso, Till; Blank, Lars M; Hayen, Heiko

    2016-09-23

    Rhamnolipids are biosurfactants produced by a variety of bacterial species that present a promising alternative to surfactants from petrochemical or oleochemical origin. The success of the fermentation is evaluated by subsequent qualitative and quantitative analysis. However, the sample preparation for high numbers of samples is often laborious and inefficient. In this study an online sample preparation is developed for the qualitative and quantitative analysis of rhamnolipids by LC-MS/MS. Online sample preparation is carried out on a TurboFlow Cyclone MAX column using turbulent flow chromatography. Sample preparation prior the analysis is minimized to a dilution and syringe filtration step leading to an instrumental analysis time of 33min. The limit of detection and the limit of quantification were 0.4ng and 0.6ng on column, respectively. Recovery of the main mono- and di-rhamnolipids from a fermentation sample was 102-104%. Additionally, the rhamnolipid biosynthetic precursors 3-hydroxy(alkanoyloxy)alkanoic acids (HAAs) are covered, albeit extraction is not quantitative (85-90%). The analysis of rhamnolipids from four different microbial species was in good agreement with previous reports. The presented method allows rapid and comprehensive analysis of rhamnolipids with minimal sample preparation directly from the fermentation broth. The application of complementary data-dependent MS/MS acquisition enables non-target screening of rhamnolipids. PMID:27567141

  3. Analysis of volatiles in Pinotage wines by stir bar sorptive extraction and chemometric profiling.

    Science.gov (United States)

    Weldegergis, Berhane T; Crouch, Andrew M

    2008-11-12

    A fast, simple, cost-effective, and reliable method based on stir bar sorptive extraction (SBSE) in the headspace mode was used for the analysis of 39 volatile components in Pinotage wines. The method was sensitive, with LODs ranging from 50.0 pg/L to 281 ng/L and LOQs between 180 pg/L and 938 ng/L. Precision was between 6 and 20%. The intermediate precision was within the acceptable range. Moreover, good calibration curves with R(2) > 0.99 for all compounds were achieved. The method was successfully applied for the analysis of 87 young Pinotage wines of vintages 2005 and 2006 collected from various South African regions. To characterize the results based on vintage and origin, the obtained concentrations of the compounds were subjected to chemometric analysis. Exploratory factor analysis (FA), principal component analysis (PCA), and analysis of variance (one-way ANOVA) were consecutively done. The chemometrics approach revealed a reasonable correlation among the volatile components of these wines, as well as with respect to their year of production.

  4. Rhamnolipid biosurfactant analysis using online turbulent flow chromatography-liquid chromatography-tandem mass spectrometry.

    Science.gov (United States)

    Behrens, Beate; Helmer, Patrick O; Tiso, Till; Blank, Lars M; Hayen, Heiko

    2016-09-23

    Rhamnolipids are biosurfactants produced by a variety of bacterial species that present a promising alternative to surfactants from petrochemical or oleochemical origin. The success of the fermentation is evaluated by subsequent qualitative and quantitative analysis. However, the sample preparation for high numbers of samples is often laborious and inefficient. In this study an online sample preparation is developed for the qualitative and quantitative analysis of rhamnolipids by LC-MS/MS. Online sample preparation is carried out on a TurboFlow Cyclone MAX column using turbulent flow chromatography. Sample preparation prior the analysis is minimized to a dilution and syringe filtration step leading to an instrumental analysis time of 33min. The limit of detection and the limit of quantification were 0.4ng and 0.6ng on column, respectively. Recovery of the main mono- and di-rhamnolipids from a fermentation sample was 102-104%. Additionally, the rhamnolipid biosynthetic precursors 3-hydroxy(alkanoyloxy)alkanoic acids (HAAs) are covered, albeit extraction is not quantitative (85-90%). The analysis of rhamnolipids from four different microbial species was in good agreement with previous reports. The presented method allows rapid and comprehensive analysis of rhamnolipids with minimal sample preparation directly from the fermentation broth. The application of complementary data-dependent MS/MS acquisition enables non-target screening of rhamnolipids.

  5. Radioimmunometric analysis of histamine in myeloproliferative syndromes

    International Nuclear Information System (INIS)

    Quantitative analysis of histamine is increasingly used in clinical haematology. The present study demonstrates the properties of and potential indication for a novel histamine radioimmunoassay (RIA) in clinical haematology. The sensitivity of this test assay corresponds to a histamine level of 0,1 to 0,5 nM, the non-specific cross reaction with endogeneous histamine metabolites appears to be less than 0,1%. The total histamine levels in the peripheral blood of healthy donors (n = 10) ranged from 10 to 100 ng/ml, the plasma histamine values from 0,02 to 0,6 ng/ml blood. Increased levels of total histamine were measured in myeloproliferative syndromes, i.e. in patients with chronic myeloid leukemia (CML)(8 of 9), myelofibrosis (OMS)(2 of 4), and polycythaemia vera (PCV)(1 of 2). An excessive increase in total histamine was observed in healthy rhesus monkeys (n = 10) treated with recombinant human interleukin-3 (rhIL-3). The total histamine value correlated with the absolute number of circulating blood basophils (correlation coefficient: 0,9). The calculated content of histamine per basophil was found to be 0,5 to 1,5 pg. Plasma histamine values in patients suffering from myeloproliferative syndromes were within the normal range. In contrast, a moderate to marked increase in plasma histamine values was observed in monkeys during IL-3 treatment. The radioimmunometric analysis of histamine clearly represents a useful new test system in clinical haematology, especially in the follow up of malignant as well as IL-3-induced myeloproliferation. (Authors)

  6. Analysis of the particular genesis of ultra-heavy oil in Shu1 area

    Energy Technology Data Exchange (ETDEWEB)

    Yu, W.; Wang, Y.; Zhang, Z.; Bao, J. [PetroChina, Liaoning (China). Liaohe Oilfield Co.

    2006-07-01

    The properties of ultra-heavy oil in the Shu1 oil reservoir in China were characterized through an analysis of geological data and laboratory experiments. The reservoir was shallow and unconsolidated with coarse glutenite lithology and high porosity and permeability. Reservoir pressures and formation temperature gradients were low, and the oil was highly viscous with a high gum and asphalt content. Oil in the Ng formation was located beneath the water and formed a round edge-top-bottom reservoir. Primary and secondary migration of the oil was caused by the fluid potential released during the hydrocarbon generation process. The tectonic history of the basin demonstrated that the sag entered a subsidence stage after the late Paleogene period. The study showed that the Shu1 ultra-heavy oil reservoir was formed long after the Ng formation, and that the accumulations in the reservoir were derived from the heat and fluid potential released during hydrocarbon generation. The study also showed that large amounts of heat loss occurred during oil migration, which caused the temperature of the Ng formation to be low, and cause oil densities to increase. It was concluded that the buoyancy of the oil generated during migration was lower than the hydrostatic fluid column pressure of the formation water, which formed a water-sealed reservoir of edge-top-bottom water. 4 refs., 3 figs.

  7. Analysis of radioactive impurities in alumina and silica used for electronic materials

    International Nuclear Information System (INIS)

    It has been established that soft error of high precision electronic circuits can be induced by alpha-particles emitted from the naturally occurring radioactive impurities such as U, Th. As the electronic circuits have recently become lower dimension and higher density, these alpha-particle emitting radioactive impurities have to be strictly controlled. The aim of this study is to develop of NAA(Neutron Activation Analysis) and gamma-spectrometry to improve the analytical sensitivity(ng/g) and precision of U and Th. New NAA methods have been established using the HTS(Hydraulic Transfer System) irradiation facility which has been used to produce radioisotopes for industries and medicines instead of PTS(Pneumatic Transfer System) irradiation facility which has been used in general NAA. When the ultra-trace impurities have to be analyzed by NAA, background gamma-ray spectra induced from 222Rn and its progeny nuclides in air is serious problem. This unstable background has been eliminated or stabilized by the use of a nitrogen purging system. Ultra trace amounts of U(0.1ng/g) and Th(0.01ng/g) in alumina ball and high purity silica used for EMC could be analyzed by the use of HTS-NAA and low background gamma-spectrometry

  8. Hair analysis as an indicator of exposure to uranium

    International Nuclear Information System (INIS)

    Medical examinations performed on four monks of a monastery in the northern Greece revealed heavy metal contamination. Hair analysis, performed by a toxicological laboratory abroad, indicated, among other, the presence of uranium. The uranium concentrations determined in a laboratory of 'Elemental Hair Analysis' indicated a uranium level that was about five times the maximum value of the reference range, which has been adopted by the measuring laboratory. After these diagnostic findings, on request of 10 monks, uranium determination in hair and urine samples was performed by means of alpha spectrometry in GAEC's laboratory. The measured uranium concentrations in hair varied from 0.15 to 2.10 mBq g-1, which correspond to 12.1 and 170 ng g-1, respectively. The uranium concentrations in urine were between 41 and 174 ng d-1. For comparison purposes, urine and non-dyed hair samples from the staff of the laboratory were analysed. Because one of the major sources of uranium intake is through drinking water, water samples were also analysed. The mean value of the uranium concentration in the two drinking water samples collected from the residence area was found to be 2.35 μg l-1. (authors)

  9. Ang Pagsasakatutubo mula sa Loob/Kultural na Pagpapatibay ng mga Salitang Pandamdaming Tumutukoy sa “Sayá”: Isang Semantikal na Elaborasyon ng Wikang Filipino sa Larangan ng Sikolohiya

    Directory of Open Access Journals (Sweden)

    Jayson D. Petras

    2013-12-01

    Full Text Available The Philippines has often been recognized as one of the most emotional countries in the world. Despite this, there is a scarcity of research pertaining to emotions in the context of Filipinos’ own language and culture; instead, the convenient practice of explaining phenomena based on studies published abroad continues. This is the reason why even local scholarship remains ethnocentric, particularly Anglocentric, in nature. This paper answers the need to contextualize studies about emotions by adopting cultural revalidation or indigenization from within that was developed by the Father of Filipino Psychology, Virgilio G. Enriquez, as an approach to the semantic elaboration of Filipino words pertaining to “happiness.” In analyzing the scope and depth of such words, as well as their similarities and differences, it is essential to consider the universal aspect of understanding emotion through the Natural Semantic Metalanguage (NSM developed by Anna Wierzbicka and her colleagues and the particular aspect embedded in the loob-labas (internal-external dimensions and the Filipino culture-specif ic use of language. Through this, highlighting the uniqueness of the words alíw, galák, ligáya, lugód, luwalhatì, sayá, siyá, tuwâ, and wíli becomes possible. In the end, the researcher poses the challenge of continuous examination of the language of emotion toward understanding Filipino personality.

  10. EFFECTS OF TEACHİNG METACOGNİTİON STRATEGİES TO LİSTENİNG COMPREHENSİON SKİLLS AND ATTİTUDE TOWARD LİSTENİNG

    OpenAIRE

    KATRANCI, Mehmet; YANGIN, BANU

    2013-01-01

    The purpose of this research is determining the effect of teaching metacognition strategies to the fifth grade students according to their listening comprehension skills and students’ attitudes toward listening. The study group of the research consists of 65 fifth grade students at two elementary schools in the province of Kırıkkale. In the study, mixed method which both quantitative and qualitative research methods are used together for collecting, analysing and interpreting data. During the...

  11. EFFECTS OF TEACHİNG METACOGNİTİON STRATEGİES TO LİSTENİNG COMPREHENSİON SKİLLS AND ATTİTUDE TOWARD LİSTENİNG

    Directory of Open Access Journals (Sweden)

    Mehmet KATRANCI

    2013-06-01

    Full Text Available The purpose of this research is determining the effect of teaching metacognition strategies to the fifth grade students according to their listening comprehension skills and students’ attitudes toward listening. The study group of the research consists of 65 fifth grade students at two elementary schools in the province of Kırıkkale. In the study, mixed method which both quantitative and qualitative research methods are used together for collecting, analysing and interpreting data. During the research process, in the experimental group, teaching metacognition strategies was carried out through the usage of listening texts activities. In the control group, listening activities were carried out according to Turkish Teaching Curriculum. Implementation process of the study was carried out for a total of 12 weeks, for two hours per week. Both control and experimental group students’ listening comprehension levels, their metacognitive awareness levels toward listening and attitudes toward listening were determined with the scale developed for the study both at the beginning and at the end of the implementation process. The data of the study were analysed with SPSS programme. According to the findings of the research, for control and experimental group students’ listening comprehension,their metacognitive awareness levels toward listening and their attitudes toward listening were found as significant difference in favor of experiment group.

  12. Simultaneous analysis method for polar and non-polar ginsenosides in red ginseng by reversed-phase HPLC-PAD.

    Science.gov (United States)

    Lee, Sa-Im; Kwon, Ha-Jeong; Lee, Yong-Moon; Lee, Je-Hyun; Hong, Seon-Pyo

    2012-02-23

    The paper describes the development of a simultaneous determination method for polar and non-polar ginsenosides in red ginseng with a reversed-phase high-performance liquid chromatography-pulsed amperometric detection method. This method could be applied directly without any pretreatment steps and enabled the performance of highly sensitive analysis within 1h. The detection (S/N=3) and quantification (S/N=10) limits for the ginsenosides ranged 0.02-0.10 ng and 0.1-0.3 ng, respectively. The linear regression coefficients ranged 0.9975-0.9998. Intra- and inter-day precisions were <9.91%. The mean recoveries ranged 98.08-103.06%. The total amount of ginsenosides in the hairy root of red ginseng was higher than that in the main root. PMID:22119615

  13. Development and validation of a method for the analysis of hydroxyzine hydrochloride in extracellular solution used in in vitro preclinical safety studies.

    Science.gov (United States)

    Briône, Willy; Brekelmans, Mari; Eijndhoven, Freek van; Schenkel, Eric; Noij, Theo

    2015-11-10

    In the process of drug development, preclinical safety studies are to be performed that require the analysis of the compound at very low concentrations with high demands on the performance of the analytical methods. In the current study, a UPLC-MS/MS method was developed and validated to quantify hydroxyzine hydrochloride in an extracellular solution used in a hERG assay in concentrations ranging from 0.01 to 10μM (4.5ng/ml-4.5μg/ml). Chromatographic separation was achieved isocratically on an Acquity BEH C18 analytical column. The assay was validated at concentrations of 0.11-1.1ng/ml in end solution for hydroxyzine hydrochloride. Linearity was demonstrated over the range of concentrations of 0.06-0.17ng/ml and over the range of concentrations of 0.6-1.7ng/ml in end solution with the coefficient of correlation r>0.99. Accuracy of the achieved concentration, intra-run, and inter-run precision of the method were well within the acceptance criteria (being mean recovery of 80-120% and relative standard deviation ≤10.0%). The limit of quantification in extracellular solution was 0.09ng/ml. Hydroxyzine hydrochloride in extracellular solution proved to be stable when stored in the fridge at 4-8°C for at least 37 days, at room temperature for at least 16 days and at +35°C for at least 16 days. The analytical method was successfully applied in hERG assay. PMID:26163869

  14. An integrated approach combining chemical analysis and an in vivo bioassay to assess the estrogenic potency of a municipal solid waste landfill leachate in Qingdao.

    Directory of Open Access Journals (Sweden)

    Yufeng Gong

    Full Text Available Various adverse effects related to landfill leachate have made leachates an important issue in past decades, and it has been demonstrated that landfill leachate is an important source of environmental estrogens. In this study, we employed chemical analysis of some already evaluated estrogenic substances, in combination with a bioassay using several specific biomarkers (e.g., plasma vitellogenin and sex steroids, enzyme activity of gonad gamma-glutamyl transpeptidase, and gonadosomatic index to evaluate the estrogenic activities in outlets from different stages of the leachate treatment process. The results indicated that 5 environmental estrogens (4-t-octylphenol, bisphenol A, di-ethyl phthalate, di-n-butyl phthalate, and diethylhexyl phthalate were detected by a gas chromatography-mass spectrometry, and the concentrations in leachate samples were 6153 ng/L, 3642 ng/L, 2139 ng/L, 5900 ng/L, and 9422 ng/L, respectively. Leachate (1∶200 diluted induced the synthesis of plasma vitellogenin and led to decreased enzyme activity of gonad gamma-glutamyl transpeptidase and gonadosomatic index in male goldfish (Carassius auratus after a 28-day exposure, while increased circulating 17β-estradiol level was also observed in males exposed to treated effluent. Although the target EEs were partially removed with removal rates varying from 87.2% to 99.77% by the "membrane bioreactor+reverse osmosis+aeration zeolite biofilter" treatment process, the treated effluent is still estrogenic to fish. The method combined chemical techniques with the responses of test organisms allowing us to identify the group of estrogen-like chemicals so that we were able to evaluate the overall estrogenic effects of a complex mixture, avoiding false negative assessments.

  15. Novel pipette-tip graphene/poly (vinyl alcohol) cryogel composite extractor for the analysis of carbofuran and carbaryl in water.

    Science.gov (United States)

    Charoenpornpukdee, Kanokrat; Thammakhet, Chongdee; Thavarungkul, Panote; Kanatharana, Proespichaya

    2014-01-01

    A novel pipette-tip extractor of a graphene/poly (vinyl alcohol) cryogel (graphene/PVA) composite sorbent was prepared to preconcentrate carbamate pesticides in environmental water samples before analysis with a gas chromatograph-flame ionization detector (GC-FID). This novel pipette-tip extractor with the graphene/PVA sorbent exhibited a high porosity when observed through a scanning electron micrograph (SEM). Under optimal conditions, using only 1.0 mL of sample and 0.75 mL of eluting solvent, the developed method provided a wide linear range of 10-700 ng mL(-1) and 10-500 ng mL(-1) with limit of detection (LOD) of 6.40 ± 0.18 and 9.17 ± 0.34 ng mL(-1) for carbofuran (2,3-dihydro-2,2-dimethylbenzofuran-7-yl methylcarbamate) and carbaryl (1-naphthyl methylcarbamate), respectively. The pipette-tip extractor provided high extraction efficiency with high accuracy indicated, by good recoveries in the range of 74.5 ± 4.8% to 119.7 ± 1.6% and 76 ± 15% to 114 ± 19% for carbofuran and carbaryl, respectively. In addition, the fabrication procedure showed a good pipette-tip extractor-to-pipette-tip extractor reproducibility with a relative standard deviation of 1.3-9.8% (n = 5). When the developed pipette-tip extractor was applied for the extraction of carbofuran and carbaryl in surface water samples near vegetable plantation areas, 25.9 ± 8.2 ng mL(-1) of carbofuran was found, and carbaryl was also detected in concentrations that ranged from 45.0 ± 4.0 to 191 ± 13 ng mL(-1).

  16. Analysis of risk factors of involvement of seminal vesicles in patients with prostate cancer undergoing radical prostatectomy

    Directory of Open Access Journals (Sweden)

    Marcos F. Dall'Oglio

    2004-12-01

    Full Text Available OBJECTIVE: To determine through preoperative serum PSA level, Gleason score on biopsy and percentage of fragments affected by tumor on biopsy, the probability of involvement of the seminal vesicles. MATERIALS AND METHODS: During the period between March 1991 to December 2002, we selected 899 patients undergoing radical prostatectomy for treatment of localized prostate adenocarcinoma. The analyzed preoperative variables were PSA, percentage of positive fragments and Gleason score on the biopsy. Pre-operative PSA was divided in scales from 0 to 4.0 ng/mL, 4.1 to 10 ng/mL, 10.1 to 20 ng/mL and > 20 ng/mL, Gleason score was categorized in scales from 2 to 6. 7 and 8 to 10, and the percentage of affected fragments was divided in 0 to 25%, 25.1% to 50%, 50.1% to 75%, and 75.1% to 100%. All these variables were correlated with the involvement of seminal vesicles in the surgical specimen. RESULTS: Of the 899 patients under study, approximately 11% (95% CI, [9% - 13%] had involvement of seminal vesicles. On the multivariate analysis, when PSA was < 4, the Gleason score was 2 to 6, and less than 25% of fragments were involved on the biopsy, only 3.6%, 7.6% and 6.2% of patients respectively, had involvement of seminal vesicles. On the multivariate analysis, we observed that PSA, Gleason score and the percentage of involved fragments were independent prognostic factors for invasion of seminal vesicles. CONCLUSION: The preoperative variables used in the present study allow the identification of men with minimal risk (lower than 5% if involvement of seminal vesicles.

  17. Bankaların Finansal Başarısızlıklarının Geleneksel ve Yeni Yöntemlerle Öngörüsü(Prediction of Financial Failure of Banks with Traditional and New Methods

    Directory of Open Access Journals (Sweden)

    Melike KURTARAN ÇELİK

    2010-01-01

    Full Text Available The aim of this study is to set the early warning models for the prediction of financial failures of banks which are most importnant factor of financial system especially in Turkey. In this study it is compared predictability of various prediction models such as discriminant analysis and artificial neural networks using usually in literature. For this purpose, financial failure probability of banks is separately predicted with financial ratios of 36 privately owned commercial banks for 1 and 2 years ago.

  18. Capillary Electrophoresis-Mass Spectrometry for the Analysis of Heparin Oligosaccharides and Low Molecular Weight Heparin.

    Science.gov (United States)

    Sun, Xiaojun; Lin, Lei; Liu, Xinyue; Zhang, Fuming; Chi, Lianli; Xia, Qiangwei; Linhardt, Robert J

    2016-02-01

    Heparins, highly sulfated, linear polysaccharides also known as glycosaminoglycans, are among the most challenging biopolymers to analyze. Hyphenated techniques in conjunction with mass spectrometry (MS) offer rapid analysis of complex glycosaminoglycan mixtures, providing detailed structural and quantitative data. Previous analytical approaches have often relied on liquid chromatography (LC)-MS, and some have limitations including long separation times, low resolution of oligosaccharide mixtures, incompatibility of eluents, and often require oligosaccharide derivatization. This study examines the analysis of glycosaminoglycan oligosaccharides using a novel electrokinetic pump-based capillary electrophoresis (CE)-MS interface. CE separation and electrospray were optimized using a volatile ammonium bicarbonate electrolyte and a methanol-formic acid sheath fluid. The online analyses of highly sulfated heparin oligosaccharides, ranging from disaccharides to low molecular weight heparins, were performed within a 10 min time frame, offering an opportunity for higher-throughput analysis. Disaccharide compositional analysis as well as top-down analysis of low molecular weight heparin was demonstrated. Using normal polarity CE separation and positive-ion electrospray ionization MS, excellent run-to-run reproducibility (relative standard deviation of 3.6-5.1% for peak area and 0.2-0.4% for peak migration time) and sensitivity (limit of quantification of 2.0-5.9 ng/mL and limit of detection of 0.6-1.8 ng/mL) could be achieved.

  19. Analysis of some egyptian cosmetic samples by using fast neutron activation analysis

    International Nuclear Information System (INIS)

    A description of our neutron generator (NG) facility for neutron activation analysis is presented. As an example, the concentration of Na, Mg, Al, Si, K, Cl, Ca and Fe elements were determined in two domestic brands of face powder by using a beam of 14 MeV neutrons. An empirical expression for detector efficiency in terms of incident gamma ray energy and the source-detector distance has been obtained for a hyper pure germanium detector (HPGe) using different standard point sources. The comparison of the calculated efficiencies and the measured values in the energy range from 59.5 to 1332.2 keV and for source-to-detector distances of 5-30 cm show the agreement between the calculated values and the measured experimental values

  20. Affordable and rapid HPTLC method for the simultaneous analysis of artemisinin and its metabolite artemisinic acid in Artemisia annua L.

    Science.gov (United States)

    Khan, Shazia; Ali, Athar; Ahmad, Shahzad; Abdin, Malik Zainul

    2015-10-01

    Artemisinin (AN) and artemisinic acid (AA), valuable phyto-pharmaceutical molecules, are well known anti-malarials, but their activities against diseases like cancer, schistosomiasis, HIV, hepatitis-B and leishmaniasis are also being reported. For the simultaneous estimation of AN and AA in the callus and leaf extracts of A. annua L. plants, we embarked upon a simple, rapid, selective, reliable and fairly economical high performance thin layer chromatography (HPTLC) method. Experimental conditions such as band size, chamber saturation time, migration of solvent front and slit width were critically studied and the optimum conditions were selected. The separations were achieved using toluene-ethyl acetate, 9:1 (v/v) as mobile phase on pre-coated silica gel plates, G 60F254 . Good resolution was achieved with Rf values of 0.35 ± 0.02 and 0.26 ± 0.02 at 536 nm for AN and 626 nm for AA, respectively, in absorption-reflectance mode. The method displayed a linear relationship with r(2) value 0.992 and 0.994 for AN and AA, respectively, in the concentration range of 300-1500 ng for AN and 200-1000 ng for AA. The method was validated for specificity by obtaining in-situ UV overlay spectra and sensitivity by estimating limit of detection (30 ng for AN and 15 ng for AA) and limit of quantitation (80 ng for AN and 45 ng for AA) values. The accuracy was checked by the recovery studies conducted at three different levels with the known concentrations and the average percentage recovery was 101.99% for AN and 103.84% for AA. The precision was analyzed by interday and intraday precision and was 1.09 and 1.00% RSD for AN and 1.22 and 6.05% RSD for AA. The analysis of statistical data substantiates that this HPTLC method can be used for the simultaneous estimation of AN and AA in biological samples. PMID:25829259

  1. Analysis of melamine migration from melamine food contact articles.

    Science.gov (United States)

    Chik, Z; Haron, D E Mohamad; Ahmad, E D; Taha, H; Mustafa, A M

    2011-01-01

    Migration of melamine has been determined for 41 types of retail melamine-ware products in Malaysia. This study was initiated by the Ministry of Health, Malaysia, in the midst of public anxiety on the possibility of melamine leaching into foods that come into contact with the melamine-ware. Thus, the objective of this study was to investigate the level of melamine migration in melamine utensils available on the market. Samples of melamine tableware, including cups and plates, forks and spoons, tumblers, bowls, etc., were collected from various retail outlets. Following the test guidelines for melamine migration set by the European Committee for Standardisation (CEN 2004) with some modifications, the samples were exposed to two types of food simulants (3% acetic acid and distilled water) at three test conditions (25°C (room temperature), 70 and 100°C) for 30 min. Melamine analysis was carried out using LC-MS/MS with a HILIC column and mobile phase consisting of ammonium acetate/formic acid (0.05%) in water and ammonium acetate/formic acid (0.05%) in acetonitrile (95 : 5, v/v). The limit of quantification (LOQ) was 5 ng/ml. Melamine migration was detected from all samples. For the articles tested with distilled water, melamine migration were [median (interquartile range)] 22.2 (32.6), 49.3 (50.9), 84.9 (89.9) ng/ml at room temperature (25°C), 70 and 100°C, respectively. In 3% acetic acid, melamine migration was 31.5 (35.7), 81.5 (76.2), 122.0 (126.7) ng/ml at room temperature (25°C), 70 and 100°C, respectively. This study suggests that excessive heat and acidity may directly affect melamine migration from melamine-ware products. However the results showed that melamine migration in the tested items were well below the specific migration limit (SML) of 30 mg/kg (30,000 ng/ml) set out in European Commission Directive 2002/72/EC. PMID:21607892

  2. Effect of Renin-Angiotensin-Aldosterone System Inhibition, Dietary Sodium Restriction, and/or Diuretics on Urinary Kidney Injury Molecule 1 Excretion in Nondiabetic Proteinuric Kidney Disease: A Post Hoc Analysis of a Randomized Controlled Trial

    Science.gov (United States)

    Waanders, Femke; Vaidya, Vishal S.; van Goor, Harry; Leuvenink, Henri; Damman, Kevin; Hamming, Inge; Bonventre, Joseph V.; Vogt, Liffert; Navis, Gerjan

    2012-01-01

    Background Tubulointerstitial damage plays an important role in chronic kidney disease (CKD) with proteinuria. Urinary kidney injury molecule 1 (KIM-1) reflects tubular KIM-1 and is considered a sensitive biomarker for early tubular damage. We hypothesized that a decrease in proteinuria by using therapeutic interventions is associated with decreased urinary KIM-1 levels. Study Design Post hoc analysis of a randomized, double-blind, placebo-controlled, crossover trial. Setting & Participants 34 proteinuric patients without diabetes from our outpatient renal clinic. Intervention Stepwise 6-week interventions of losartan, sodium restriction (low-sodium [LS] diet), their combination, losartan plus hydrochlorothiazide (HCT), and the latter plus an LS diet. Outcomes & Measurements Urinary excretion of KIM-1, total protein, and N-acetyl-β-D-glucosaminidase (NAG) as a positive control for tubular injury. Results Mean baseline urine protein level was 3.8 ± 0.4 (SE) g/d, and KIM-1 level was 1,706 ± 498 ng/d (increased compared with healthy controls; 74 ng/d). KIM-1 level was decreased by using placebo/LS (1,201 ± 388 ng/d; P = 0.04), losartan/high sodium (1,184 ± 296 ng/d; P = 0.09), losartan/LS (921 ± 176 ng/d; P = 0.008), losartan/high sodium plus HCT (862 ± 151 ng/d; P = 0.008) and losartan/LS plus HCT (743 ± 170 ng/d; P = 0.001). The decrease in urinary KIM-1 levels paralleled the decrease in proteinuria (R = 0.523; P < 0.001), but not blood pressure or creatinine clearance. 16 patients reached target proteinuria with protein less than 1 g/d, whereas KIM-1 levels normalized in only 2 patients. Urinary NAG level was increased at baseline and significantly decreased during the treatment periods of combined losartan plus HCT only. The decrease in urinary NAG levels was not closely related to proteinuria. Limitations Post hoc analysis. Conclusions Urinary KIM-1 level was increased in patients with nondiabetic CKD with proteinuria and decreased in parallel with

  3. Pilipino Kindergarten Language Arts--Needs. Teacher's Handbook [and] Teacher's Answer Key [and] Student Workbook=Sining ng Wika Pangkindergarten--Mga Pangangailangan. Hanbuk ng Guro.

    Science.gov (United States)

    California State Univ., Los Angeles. National Dissemination and Assessment Center.

    As part of the Asian American Bilingual Center (AABC) Pilipino Language Arts Program, a curriculum suitable for students in kindergarten is presented. The program is geared both to the immigrant Filipino child and to the American-born Filipino child who learned to speak Pilipino at home, and includes kits that emphasize the AABC's major concepts:…

  4. Safety and immunogenicity of hepatitis B vaccine ButaNG in adults Inocuidade e imunogenicidade da vacina contra a hepatite B, ButaNG, em adultos

    Directory of Open Access Journals (Sweden)

    Luzia M. IOSHIMOTO

    1999-05-01

    Full Text Available Recombinant yeast-derived hepatitis B vaccine manufactured by Instituto Butantan was administered in two groups of adult volunteers (I, II following two different schedules of immunization. In the first trial (10 mg doses and 0, 1, 3 months vaccination schedule 106 individuals completed the full immunization program. The results of seroconversion by age group varied from 70 to 100% and the GMT from 46.5 to 124.9 mIU mL-1. In the second trial with 68 individuals (for dosage comparison and 0, 1, 6 months vaccination schedule indicated that the vaccine formulated in 20 mg was more effective than in 10 mg. The adverse reactions observed in the vaccinees were less frequent than the ones previously found since the introduction of similar vaccines.Vacina contra a hepatite B, produzida no Instituto Butantan em levedura recombinante, foi administrada em dois grupos de voluntários adultos (I e II seguindo dois esquemas diferentes de imunização. No primeiro ensaio (doses de 10 mg e esquema de vacinação de 0, 1, 3 meses, 106 indivíduos completaram o programa de imunização proposto. Os resultados de soroconversão agrupados por faixa etária variaram de 70 a 100%, enquanto que o TGM foi de 46,5 a 124 mUI mL-1. No segundo ensaio com 68 indivíduos (comparação de doses e esquema de vacinação de 0, 1, 6 meses os resultados indicaram que a vacina formulada em dose de 20 mg foi mais eficaz que em dose de 10 mg. Os efeitos adversos observados nos vacinados foram menores do que os relatados por outros autores, desde o início da aplicação de vacinas similares.

  5. 76 FR 27861 - Airworthiness Directives; Diamond Aircraft Industries GmbH Models DA 42, DA 42 NG, and DA 42 M-NG...

    Science.gov (United States)

    2011-05-13

    ... (76 FR 12627). That NPRM proposed to correct an unsafe condition for the specified products. The MCAI...-16534 (75 FR 75868, December 7, 2010), as a unilateral action to address this unsafe condition on Models... Regulatory Policies and Procedures (44 FR 11034, February 26, 1979); and (3) Will not have a...

  6. Development of cancer therapy facility of HANARO and medical research in BNCT; development of the technique for boron concentration analysis

    Energy Technology Data Exchange (ETDEWEB)

    Choi, Hee Dong; Byun, Soo Hyun; Sun, Gwang Min; Kim, Suk Kwon; Kim, In Jung; Park, Chang Su [Seoul National University, Seoul (Korea)

    2002-03-01

    Objective and Necessity of the Project- Development of a boron concentration analysis facility used for BNCT. - Development of the technique for boron concentration analysis. Contents and Scopes of the Project - Construction of the boron concentration analysis facility based on PGAA. Estimation of the neutron beam characteristics. -Establishment of the technique for the boron concentration analysis. - Estimation of the reliability for the boron analysis. Results of the Project -Installation of the boron concentration analysis facility at Hanaro. - Neutron beam characteristics are the sample position (neutron flux : 7.9 x 10{sup 7} n/cm{sup 2}s, Cd-ratio : 266) Technique for the boron concentration analysis. - Boron detection sensitivity and limit (detection sensitivity : 2, 131 cps/mg-B, detection limit : 67 ng for 10,000 sec). 63 refs., 37 figs., 13 tabs. (Author)

  7. Türkiye’de Enflasyonun İleri ve Geri Beslemeli Yapay Sinir Ağlarının Melez Yaklaşımı ile Öngörüsü = Forecasting of Turkey Inflation with Hybrid of Feed Forward and Recurrent Artifical Neural Networks

    Directory of Open Access Journals (Sweden)

    V. Rezan USLU

    2010-01-01

    Full Text Available Obtaining the inflation prediction is an important problem. Having this prediction accurately will lead to more accurate decisions. Various time series techniques have been used in the literature for inflation prediction. Recently, Artificial Neural Network (ANN is being preferred in the time series prediction problem due to its flexible modeling capacity. Artificial neural network can be applied easily to any time series since it does not require prior conditions such as a linear or curved specific model pattern, stationary and normal distribution. In this study, the predictions have been obtained using the feed forward and recurrent artificial neural network for the Consumer Price Index (CPI. A new combined forecast has been proposed based on ANN in which the ANN model predictions employed in analysis were used as data.

  8. Identification and analysis of piperitone in red wines.

    Science.gov (United States)

    Pons, Alexandre; Lavigne, Valérie; Darriet, Philippe; Dubourdieu, Denis

    2016-09-01

    The present study concerns the search for new aroma compounds associated with the flavor of aged and prematurely aged red wines. Sensory descriptive analysis associated with gas chromatography-olfactometry was first performed to find specific odoriferous zones. One of the zones, reminiscent of mint, was found in red wines with a prune flavor. Thanks to several analytical approaches including preparative gas chromatography, p-menth-1-en-3-one (piperitone), has been identified in red wines. The olfactory detection threshold of this terpene reminiscent of mint was 0.9μg/L in model hydroalcoholic solution and 70μg/L in red wines. Chiral multidimensional GC-MS was used to show that piperitone was present mainly in the (R) form in red wines. The concentration of piperitone in aged red wines ranged from a few ng/L to 435ng/L, but the level was not linked to the age of the wine nor to its premature evolution. PMID:27041315

  9. A microfluidic dialysis device for complex biological mixture SERS analysis

    KAUST Repository

    Perozziello, Gerardo

    2015-08-01

    In this paper, we present a microfluidic device fabricated with a simple and inexpensive process allowing rapid filtering of peptides from a complex mixture. The polymer microfluidic device can be used for sample preparation in biological applications. The device is fabricated by micromilling and solvent assisted bonding, in which a microdialysis membrane (cut-off of 12-14 kDa) is sandwiched in between an upper and a bottom microfluidic chamber. An external frame connects the microfluidic device to external tubes, microvalves and syringe pumps. Bonding strength and interface sealing are pneumatically tested. Microfluidic protocols are also described by using the presented device to filter a sample composed of specific peptides (MW 1553.73 Da, at a concentration of 1.0 ng/μl) derived from the BRCA1 protein, a tumor-suppressor molecule which plays a pivotal role in the development of breast cancer, and albumin (MW 66.5 kDa, at a concentration of 35 μg/μl), the most represented protein in human plasma. The filtered samples coming out from the microfluidic device were subsequently deposited on a SERS (surface enhanced Raman scattering) substrate for further analysis by Raman spectroscopy. By using this approach, we were able to sort the small peptides from the bigger and highly concentrated protein albumin and to detect them by using a label-free technique at a resolution down to 1.0 ng/μl.

  10. Oxytetracycline analysis in honey using a specific portable analyzer

    Science.gov (United States)

    Chen, Guoying; Schwartz, Daniel; Braden, S.; Nunez, Alberto

    2007-09-01

    Oxytetracycline (OTC) residue in honey is detected using a portable analyzer designed to specifically target tetracycline (TC) drugs based on europium-sensitized luminescence (ESL). A 385 nm light emitting diode (LED) is used as the excitation source and a photomultiplier tube as the light detector. OTC is extracted from honey and cleaned up by solid phase extraction (SPE) using Strata X-WC weak cation exchange cartridges. To the eluate Eu(III) is added to form a Eu-TC chelate at pH 8.5. Efficient intrachelate energy transfer allows sensitive OTC detection at λ ex=385 nm and λ em=610 nm. After a 25-µs time delay, the ESL signal is integrated over a 25-1000 µs interval. The signal intensity reveals a linear relationship (R2=0.972) to OTC concentrations in the 10-200 ng/g range. The limit-of-detection is 6.7 ng/g with an average 5.8% relative standard deviation. The background signal corresponds to ~10 ppb. This instrumentation and method combination enables field analysis that is especially useful for beekeeping industry.

  11. Magnetic-encoded fluorescent multifunctional nanospheres for simultaneous multicomponent analysis.

    Science.gov (United States)

    Song, Erqun; Han, Weiye; Li, Jingrong; Jiang, Yunfei; Cheng, Dan; Song, Yang; Zhang, Pu; Tan, Weihong

    2014-10-01

    In this study, magnetic-encoded fluorescent (CdTe/Fe3O4)@SiO2 multifunctional nanospheres were constructed by adjusting the initial concentration of Fe3O4 in a fabrication process based on reverse microemulsion. The resultant multifunctional nanospheres were characterized by transmission electron microscopy, X-ray diffraction measurements, fluorescence spectrophotometry, and vibrating sample magnetometry. They showed good fluorescence properties, gradient magnetic susceptibility (weak, moderate, and strong), and easy biofunctionalization for biomolecules, such as immunoglobulin G (IgG), protein, and antibody. Then the capture efficiency of the (CdTe/Fe3O4)@SiO2 nanospheres were investigated by using the fluorophore-labeled IgG-conjugated nanospheres as a model. Further studies demonstrated the ability of these (CdTe/Fe3O4)@SiO2 multifunctional nanospheres to accomplish sequentially magnetic separation, capture, and fluorescent detection for each corresponding antigen of CA125, AFP, and CEA with a detection limit of 20 KU/L, 10 ng/mL, and 5 ng/mL, respectively, from a mixed sample under a certain external magnetic field within a few minutes. The strategy of combining magnetic-encoding-based separation and fluorescence-based detection proposed in this study shows great potential to achieve easy, rapid, economical, and near-simultaneous multicomponent separation and analysis for a variety of targets such as drugs, biomarkers, pathogens, and so on. PMID:25197942

  12. Trace analysis measurements in high-purity aluminium by means of radiochemical neutron and proton activation analysis

    International Nuclear Information System (INIS)

    The aim of the study consisted in the development of efficient radiochemical composite processes and activation methods for the multi-element determination of traces within the lower ng range in high-purity aluminium. More than 50 elements were determined with the help of activation with reactor neutrons; the selective separation of matrix activity (adsorption with hydrated antimony pentoxide) led to a noticeable improvement of detectability, as compared with instrumental neutron activation analysis. Further improvements were achieved with the help of radiochemical group separations in ion exchangers or with the help of the selective separation of the pure beta-emitting elements. Over 20 elements up to high atomic numbers were determined by means of activating 13 MeV protons and 23 Me protons. In this connection, improvements of the detection limit by as a factor of 10 were achieved with radiochemical separation techniques, as compared with pure instrumental proton activation analysis. (RB)

  13. Direct Analysis of Amphetamine Stimulants in a Whole Urine Sample by Atmospheric Solids Analysis Probe Tandem Mass Spectrometry

    Science.gov (United States)

    Crevelin, Eduardo J.; Salami, Fernanda H.; Alves, Marcela N. R.; De Martinis, Bruno S.; Crotti, Antônio E. M.; Moraes, Luiz A. B.

    2016-05-01

    Amphetamine-type stimulants (ATS) are among illicit stimulant drugs that are most often used worldwide. A major challenge is to develop a fast and efficient methodology involving minimal sample preparation to analyze ATS in biological fluids. In this study, a urine pool solution containing amphetamine, methamphetamine, ephedrine, sibutramine, and fenfluramine at concentrations ranging from 0.5 pg/mL to 100 ng/mL was prepared and analyzed by atmospheric solids analysis probe tandem mass spectrometry (ASAP-MS/MS) and multiple reaction monitoring (MRM). A urine sample and saliva collected from a volunteer contributor (V1) were also analyzed. The limit of detection of the tested compounds ranged between 0.002 and 0.4 ng/mL in urine samples; the signal-to-noise ratio was 5. These results demonstrated that the ASAP-MS/MS methodology is applicable for the fast detection of ATS in urine samples with great sensitivity and specificity, without the need for cleanup, preconcentration, or chromatographic separation. Thus ASAP-MS/MS could potentially be used in clinical and forensic toxicology applications.

  14. Analysis of Digoxin and Metildigoxin in Whole Blood Using Solid-Phase Extraction and Liquid Chromatography Tandem Mass Spectrometry

    Directory of Open Access Journals (Sweden)

    Paula Melo

    2012-01-01

    Full Text Available A simple and rapid UPLC/MS/MS method has been developed and validated for the analysis of digoxin and metildigoxin in whole blood. Samples were prepared by SPE extraction with Oasis HLB columns. Separation was achieved with an ACQUITY UPLC HSS T3 column (2.1×100; 1.8 μm, at 35°C. The mobile phase consisted of acetonitrile (70% and ammonium formate 5 mM (30%. Total run time was 1.5 min operating at isocratic mode with a flow rate of 0.3 mL/min. Mass spectrometry detection was performed by positive mode electrospray, on the ammonium adducts with two transitions for each analyte and one for the IS (d3-digoxin. The method proved to be specific and linear over the range (0.3–10 ng/mL. This technique also showed high sensitivity with a 0.09 ng/mL LOD and a 0.28 ng/mL LOQ, for both substances. Percentage recovery ranged from 83 to 100% for digoxin and from 62 to 94% for metildigoxin. The intra- and interday precision CV were ≤10%.

  15. An improved method for the analysis of GHB in human hair by liquid chromatography tandem mass spectrometry.

    Science.gov (United States)

    Jagerdeo, Eshwar; Montgomery, Madeline A; LeBeau, Marc A

    2015-03-01

    The abuse of gamma-hydroxybutyric acid (GHB) and its suspicion in cases of suspected drug-facilitated sexual assault is of keen interest to forensic toxicology laboratories. This paper reports an extraction, separation and detection procedure for GHB in hair utilizing a combination of liquid-liquid extraction and solid-phase extraction using ethyl acetate and Oasis Max(®) cartridge, respectively, after the hair sample was digested. Analysis was by LC-MS-MS using a gradient separation on an Acclaim(®) Trinity(TM) P1 column performing three multiple-reaction monitoring (MRM) transitions each for GHB and its internal standard. The procedure was validated over a range from 0.4 to 50 ng/mg with estimated limit of detection (LOD) of 0.33 and an administratively set limit of quantitation (LOQ) of 1.2 ng/mg. Twenty hair specimens collected from individuals with no known exposure to GHB were analyzed for matrix interferences and to establish initial background levels of GHB. A wide range of endogenous GHB levels were observed in these samples (from less than the LOQ to 4.4 ng/mg). The results suggest the need for additional studies to better establish the full range of endogenous GHB levels in hair and that extreme caution is required in interpreting GHB findings in hair samples.

  16. Extraction of aflatoxins from liquid foodstuff samples with polydopamine-coated superparamagnetic nanoparticles for HPLC-MS/MS analysis.

    Science.gov (United States)

    McCullum, Cassandra; Tchounwou, Paul; Ding, Li-Sheng; Liao, Xun; Liu, Yi-Ming

    2014-05-14

    A facile magnetic solid phase extraction (MSPE) of aflatoxins (AFs) from liquid samples was developed using polydopamine-coated magnetic nanoparticles (PD-MNPs) as the adsorbent. PD-MNPs were prepared from amine-terminated MNPs and dopamine via an in situ oxidative self-polymerization approach. Under the selected MSPE conditions, extraction yields ranging from 59.3% for AF G2 to 89.0% for AF B1 were obtained with good repeatability. Coupled with HPLC-MS/MS quantification, the MSPE procedure serves not only for sample cleanup but also for AFs enrichment that is highly desired for trace analysis. The proposed MSPE-HPLC-MS/MS method had a linear calibration curve in the concentration range from 0.00600 to 3.00 ng/mL aflatoxin and limits of detection of 0.0012 ng/mL for AF B1, AF B2, and AF G1, and 0.0031 ng/mL for AF G2.

  17. Analysis of aflatoxins in nonalcoholic beer using liquid-liquid extraction and ultraperformance LC-MS/MS.

    Science.gov (United States)

    Khan, Mohammad R; Alothman, Zeid A; Ghfar, Ayman A; Wabaidur, Saikh M

    2013-02-01

    Aflatoxins AFB1, AFB2, AFG1, and AFG2 are toxic secondary metabolites produced by Aspergillus flavus and Aspergillus parasiticus and posses a potential threat to food safety. In the present work, liquid-liquid extraction and ultraperformance LC-MS/MS method has been applied for the determination of four naturally occurring aflatoxins AFB1, AFB2, AFG1, and AFG2 in nonalcoholic beer. Aflatoxins extraction from nonalcoholic beer was carried out using liquid-liquid extraction procedure. The effects of solvent-types were studied to obtain maximum recovery of the target analytes with minimum contamination. Among different solvents, the aflatoxins extraction was best achieved using ethyl acetate. The obtained recoveries were ranged from 85 to 96% with good quality parameters: LOD values between 0.001 and 0.003 ng/mL, linearity of the calibration curve (r(2) > 0.999), and repeatability (run-to-run) and reproducibility (day-to-day) precisions with RSDs lower than 5% (n = 5) achieved at 0.50 ng/mL concentration. The optimized liquid-liquid extraction in combination with ultraperformance LC-MS/MS was applied successfully to the analysis of AFB1, AFB2, AFG1, and AFG2 aflatoxins in 11 nonalcoholic beers and were detected up to 15.31 ng/L in some of the samples.

  18. Liquid Chromatography-Tandem Mass Spectrometry Analysis of Perfluorooctane Sulfonate and Perfluorooctanoic Acid in Fish Fillet Samples

    Directory of Open Access Journals (Sweden)

    Viviana Paiano

    2012-01-01

    Full Text Available Perfluorooctane sulfonate (PFOS and perfluorooctanoic (PFOA acid are persistent contaminants which can be found in environmental and biological samples. A new and fast analytical method is described here for the analysis of these compounds in the edible part of fish samples. The method uses a simple liquid extraction by sonication, followed by a direct determination using liquid chromatography-tandem mass spectrometry (LC-MS/MS. The linearity of the instrumental response was good, with average regression coefficients of 0.9971 and 0.9979 for PFOS and PFOA, respectively, and the coefficients of variation (CV of the method ranged from 8% to 20%. Limits of detection (LOD were 0.04 ng/g for both the analytes and recoveries were 90% for PFOS and 76% for PFOA. The method was applied to samples of homogenized fillets of wild and farmed fish from the Mediterranean Sea. Most of the samples showed little or no contamination by perfluorooctane sulfonate and perfluorooctanoic acid, and the highest concentrations detected among the fish species analyzed were, respectively, 5.96 ng/g and 1.89 ng/g. The developed analytical methodology can be used as a tool to monitor and to assess human exposure to perfluorinated compounds through sea food consumption.

  19. Extraction of aflatoxins from liquid foodstuff samples with polydopamine-coated superparamagnetic nanoparticles for HPLC-MS/MS analysis.

    Science.gov (United States)

    McCullum, Cassandra; Tchounwou, Paul; Ding, Li-Sheng; Liao, Xun; Liu, Yi-Ming

    2014-05-14

    A facile magnetic solid phase extraction (MSPE) of aflatoxins (AFs) from liquid samples was developed using polydopamine-coated magnetic nanoparticles (PD-MNPs) as the adsorbent. PD-MNPs were prepared from amine-terminated MNPs and dopamine via an in situ oxidative self-polymerization approach. Under the selected MSPE conditions, extraction yields ranging from 59.3% for AF G2 to 89.0% for AF B1 were obtained with good repeatability. Coupled with HPLC-MS/MS quantification, the MSPE procedure serves not only for sample cleanup but also for AFs enrichment that is highly desired for trace analysis. The proposed MSPE-HPLC-MS/MS method had a linear calibration curve in the concentration range from 0.00600 to 3.00 ng/mL aflatoxin and limits of detection of 0.0012 ng/mL for AF B1, AF B2, and AF G1, and 0.0031 ng/mL for AF G2. PMID:24766417

  20. Antibiotics in the coastal environment of the Hailing Bay region, South China Sea: Spatial distribution, source analysis and ecological risks

    International Nuclear Information System (INIS)

    Highlights: • Thirty-eight antibiotics were systematically investigated in marine environment. • The distribution of antibiotics was significantly correlated with COD and NO3–N. • Untreated domestic sewage was the primary source of antibiotics. • Fluoroquinolones showed a strong sorption capacity onto sediments. • Oxytetracycline, norfloxacin and erythromycin–H2O indicated high risks. - Abstract: In this study, the occurrence and spatial distribution of 38 antibiotics in surface water and sediment samples of the Hailing Bay region, South China Sea, were investigated. Twenty-one, 16 and 15 of 38 antibiotics were detected with the concentrations ranging from <0.08 (clarithromycin) to 15,163 ng/L (oxytetracycline), 2.12 (methacycline) to 1318 ng/L (erythromycin–H2O), <1.95 (ciprofloxacin) to 184 ng/g (chlortetracycline) in the seawater, discharged effluent and sediment samples, respectively. The concentrations of antibiotics in the water phase were correlated positively with chemical oxygen demand and nitrate. The source analysis indicated that untreated domestic sewage was the primary source of antibiotics in the study region. Fluoroquinolones showed strong sorption capacity onto sediments due to their high pseudo-partitioning coefficients. Risk assessment indicated that oxytetracycline, norfloxacin and erythromycin–H2O posed high risks to aquatic organisms

  1. Immunochemical analysis of fumigaclavine mycotoxins in respiratory tissues and in blood serum of birds with confirmed aspergillosis.

    Science.gov (United States)

    Latif, Hadri; Gross, Madeleine; Fischer, Dominik; Lierz, Michael; Usleber, Ewald

    2015-11-01

    The ergoline alkaloid fumigaclavine A (FuA) is one of the major mycotoxins produced by Aspergillus fumigatus, the main causative fungal agent of avian aspergillosis. To study in situ production of FuA, post-mortem respiratory tissues of various avian species, as well as blood samples of falcons (Falco sp.), were analysed by enzyme immunoassay (EIA). At a detection limit of 1.5 ng/ml, FuA EIA positive results were obtained for tissue samples from seven (64%) out of 11 birds with confirmed aspergillosis, with a maximum concentration of 38 ng/g, while all controls (n = 7) were negative. No FuA could be detected in blood serum (detection limit 0.7 ng/ml) of 15 falcons, experimentally inoculated with A. fumigatus conidia. Fungal mycelium material from tissue of clinical aspergillosis cases, cultured on malt extract agar, was highly positive in the FuA EIA in milligrams per gram range. Chromatographic analysis of mycelium extracts revealed the co-presence of FuA and the structurally related fumigaclavine C (FuC). Alkaline hydrolysis of FuA and FuC yielded the corresponding deacetylation products, FuB and FuE. This is the first report showing that fumigaclavine alkaloids are produced by A. fumigatus in situ during the course of clinical aspergillosis in birds; however, the role of these compounds in the pathogenesis of this disease is still unknown.

  2. A novel vacuum spectrometer for total reflection x-ray fluorescence analysis with two exchangeable low power x-ray sources for the analysis of low, medium, and high Z elements in sequence

    Energy Technology Data Exchange (ETDEWEB)

    Wobrauschek, P., E-mail: wobi@ati.ac.at; Prost, J.; Ingerle, D.; Kregsamer, P.; Streli, C. [Atominstitut, TU Wien, Stadionallee 2, 1020 Vienna (Austria); Misra, N. L. [Fuel Chemistry Division, Bhabha Atomic Research Centre (BARC), Mumbai 400085 (India)

    2015-08-15

    The extension of the detectable elemental range with Total Reflection X-ray Fluorescence (TXRF) analysis is a challenging task. In this paper, it is demonstrated how a TXRF spectrometer is modified to analyze elements from carbon to uranium. Based on the existing design of a vacuum TXRF spectrometer with a 12 specimen sample changer, the following components were renewed: the silicon drift detector with 20 mm{sup 2} active area and having a special ultra-thin polymer window allowing the detection of elements from carbon upwards. Two exchangeable X-ray sources guarantee the efficient excitation of both low and high Z elements. These X-ray sources were two light-weighted easily mountable 35 W air-cooled low-power tubes with Cr and Rh anodes, respectively. The air cooled tubes and the Peltier-cooled detector allowed to construct a transportable tabletop spectrometer with compact dimensions, as neither liquid nitrogen cooling for the detector nor a water cooling circuit and a bulky high voltage generator for the X-ray tubes are required. Due to the excellent background conditions as a result of the TXRF geometry, detection limits of 150 ng for C, 12 ng for F, and 3.3 ng for Na have been obtained using Cr excitation in vacuum. For Rh excitation, the detection limits of 90 pg could be achieved for Sr. Taking 10 to 20 μl of sample volume, extrapolated detection limits in the ng/g (ppb) range are resulting in terms of concentration.

  3. Stable Isotope Dilution Analysis of Gibberellin Residues in Tomato Paste by Liquid Chromatography-Tandem Mass Spectrometry

    Institute of Scientific and Technical Information of China (English)

    SUN Li; ZHAO Yan-sheng; NIE Xue-mei; LING Yun; CHU Xiao-gang; SHANG De-jun; DONG Ying

    2012-01-01

    An accurate and sensitive method for the simultaneous determination of gibberellic acid(GA3),gibberellin A4(GA4) and gibberellin A7(GA7) residues in tomato paste was developed by coupling solid phase extraction to high performance liquid chromatography-tandem mass spectrometry(LC-MS/MS) with electrospray ionization based stable isotope dilution analysis(SIDA).The isotope labeled internal standard can compensate for the losses during the extraction and cleanup steps and for discrimination due to ion suppression.After extraction from methanol,hydrophile lipophilic balance(HLB) solid phase extraction(SPE) column was tested for the capacity of the cleanup of the tomato paste in compared with C18 SPE column which is the common way to the detection of GAs,and the former gained better result.Spiked experiments were performed in the non-contaminated tomato pastes and the recoveries of GA3,GA4 and GA7 were 42.6%-75.0% in external standard method(ESM) and 91.1%-103.8% in internal standard method(ISM) respectively.The validities of this method were investigated and good analytical performance for the three GAs was obtained,including low limits of method detection(2 ng/g for GA3 and GA4,0.3 ng/g for GA7),excellent linear dynamic ranges(5-500 ng/g for GA3 and GA4,1-100 ng/g for GAy) and good relative standard deviation ranges(4.8%-9.4% for the intra-day test and 3.5%-11.9% for the inter-day test).

  4. Validation of an analytical methodology for the quantitative analysis of petroleum hydrocarbons in marine sediment samples

    OpenAIRE

    Eloy Yordad Companioni Damas; Miriam Odette Cora Medina; Ana Catalina Núñez Clemente; Miguel Ángel Díaz Díaz; Luis González Bravo; Rolando Marbot Ramada; Rodny Montes de Oca Porto

    2009-01-01

    This work describes a validation of an analytical procedure for the analysis of petroleum hydrocarbons in marine sediment samples. The proposed protocol is able to measure n-alkanes and polycyclic aromatic hydrocarbons (PAH) in samples at concentrations as low as 30 ng/g, with a precision better than 15% for most of analytes. The extraction efficiency of fortified sediments varied from 65.1 to 105.6% and 59.7 to 97.8%, for n-alkanes and PAH in the ranges: C16 - C32 and fluoranthene - benzo(a)...

  5. Radiometric-microbiologic assay of niacin using Kloeckera brevis: analysis of human blood and food

    Energy Technology Data Exchange (ETDEWEB)

    Guilarte, T.R.; Pravlik, K.

    1983-12-01

    Kloeckera brevis, a yeast, was used as the test organism for the development of a radiometric-microbiologic (RMA) assay for niacin. The assay was determined to be sensitive to the 2 ng niacin per vial level and specific for the biologically active forms of this vitamin. The method was shown to be simple, accurate, and precise in the analysis of niacin in human blood and food. The application of the radiometric technique eliminates some of the problems encountered with conventional turbidimetric-microbiologic assay.

  6. The Computer Aided Integrated Design and Analysis of Oil Tank in Vehicle

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    This paper creates 3D solid model and assembly of U RJ 92-6 oil tank and analyses its strength by integrated CAD/CAE/CAM software I-D EAS. Through integrated simulation in computer, design efficiency and quality of oil tank is greatly improved. Adopting integrated CAD/CAE/CAM software to carry out integrated research to equ ipment and products, we will be able to take overall analysis in aspects of 3-D solid modeling, pre-assembly and strength, etc., to realize non-paper designi ng and parallel designing...

  7. Advanced inductively coupled plasma mass spectrometry analysis of rare earth elements: environmental applications

    OpenAIRE

    Zhu, W.

    1999-01-01

    Rare Earth Elements (REEs) are a group of 16 related elements with low concentrations in air particulate matter, soil and water. An analytical technique was devised capable of measuring simultaneously all 16 REEs at very low levels and at great speed in environmental matrices. The ICP-MS in combination with the PLSR model and ion exchange preconcentration offers a fast multi-element analysis technique with high precision and accuracy and detection limits of below 1 ng.r\\ REEs were analysed in...

  8. Analysis of Mercury-Rich plants and mine tailings using the Hydride-Generation AAS method

    OpenAIRE

    Fábio Netto Moreno; Christopher Anderson; Robert Stewart; Brett Robinson

    2009-01-01

    A hydride-generation atomic absorption spectroscopy (AAS) method was developed for the analysis of total Hg in liquid matrices of mercury-rich plants and mine tailings samples. The detection limit for this method was as low as 11.4 ng/mL. The reproducibility of the mercury signals (in terms of relative standard deviation) was 4.6%. Accuracy of the method was verified by analyses of deionised water samples spiked with HgCl2 and HgNO3. Recovery values for total mercury ranged from 88.5 to 94.3%...

  9. Nondestructive determination of arsenic in urine by epithermal neutron activation analysis and Compton suppression.

    Science.gov (United States)

    Landsberger, S; Swift, G; Neuhoff, J

    1990-01-01

    Epithermal neutron activation analysis, in conjunction with Compton suppression, has been employed to determine arsenic levels in artificially doped urine samples. Typical detection limits were of the order of 10 ng/g. Replicate determinations gave precision values between 2 and 12%, whereas accuracy measurements were between +/- 1 and +/- 20%. Biological and geological reference materials from the National Institute of Standards and Technology (NIST) were also analyzed for arsenic content. Typically, the precision achieved again was between 2 and 12%, whereas the accuracy measurements were in excellent agreement with the certified values. PMID:1704729

  10. THE EFFECT OF AEROBİC EXERCİSE ON HS-CRP AND BODY COMPOSİTİON İNDEXES İN NON-ACTİVE OBESE MEN WİTH EMPHASİS ON RAMADAN FASTİNG

    Directory of Open Access Journals (Sweden)

    Amin

    2014-08-01

    Full Text Available Introduction: Purpose of the study was to evaluate the effect of aerobic exercise and Ramadan fasting on the high-sensitivity C-reactive protein as a marker of inflammation in non-active obese men. Methods and Materials: In this study, 18 obese men aged 50-40with a BMI over 30 kg per square meter as a public call among the 70 subjects were selected randomly and after it were divided in to fasting (N=9 and fasting and exercise (N=9 groups. Then while the first group would do only fasting, fasting and exercise group in addition to the intervention fasting group, exercise to be carried out in 27 sessions. Also, for check the desired changes in the month of Ramadan, blood samples were taken from four different times. Finally, using repeated measures analysis of variance in the level of p <0/05 theories were put to the test. Results: Ramadan fasting significantly decreased the high-sensitivity C-reactive protein (p < 0.05 as well as aerobic exercise significantly were reduced high-sensitivity C-reactive protein (p < 0.05 in both groups. Conclusion: aerobic exercise and Ramadan fasting has a beneficial effect on the reduction of low-grade inflammation.

  11. Simultaneous analysis of multiple classes of antimicrobials in environmental water samples using SPE coupled with UHPLC-ESI-MS/MS and isotope dilution.

    Science.gov (United States)

    Tran, Ngoc Han; Chen, Hongjie; Do, Thanh Van; Reinhard, Martin; Ngo, Huu Hao; He, Yiliang; Gin, Karina Yew-Hoong

    2016-10-01

    A robust and sensitive analytical method was developed for the simultaneous analysis of 21 target antimicrobials in different environmental water samples. Both single SPE and tandem SPE cartridge systems were investigated to simultaneously extract multiple classes of antimicrobials. Experimental results showed that good extraction efficiencies (84.5-105.6%) were observed for the vast majority of the target analytes when extraction was performed using the tandem SPE cartridge (SB+HR-X) system under an extraction pH of 3.0. HPLC-MS/MS parameters were optimized for simultaneous analysis of all the target analytes in a single injection. Quantification of target antimicrobials in water samples was accomplished using 15 isotopically labeled internal standards (ILISs), which allowed the efficient compensation of the losses of target analytes during sample preparation and correction of matrix effects during UHPLC-MS/MS as well as instrument fluctuations in MS/MS signal intensity. Method quantification limit (MQL) for most target analytes based on SPE was below 5ng/L for surface waters, 10ng/L for treated wastewater effluents, and 15ng/L for raw wastewater. The method was successfully applied to detect and quantify the occurrence of the target analytes in raw influent, treated effluent and surface water samples. PMID:27474294

  12. Development of aptamer-conjugated magnetic graphene/gold nanoparticle hybrid nanocomposites for specific enrichment and rapid analysis of thrombin by MALDI-TOF MS.

    Science.gov (United States)

    Xiong, Ya; Deng, Chunhui; Zhang, Xiangmin

    2014-11-01

    Simple, rapid and sensitive analysis of thrombin (a tumor biomarker) in complex samples is quite clinical relevant and essential for the development of disease diagnosis and pharmacotherapy. Herein, we developed a novel method based on aptamer-conjugated magnetic graphene/gold nanoparticles nanocomposites (MagG@Au) for specific enrichment and rapid analysis of thrombin in biological samples using MALDI-TOF-MS. At first, gold nanoparticles were compactly deposited on PDDA functionalized magnetic graphene through electrostatic interaction. Afterwards, aptamer was easily conjugated to gold nanoparticles via Au-S bond formation. The as-made aptamer-conjugated nanocomposites took advantage of the magnetism of magnetic graphene, the high affinity and specificity of aptamer, facilitating a high-efficient separation and enrichment of thrombin. More importantly, due to the large surface area of the hybrid substrate, the average coverage density of aptamer achieved 0.34 nmol/mg, which enhanced the thrombin binding capacity and the recovery of thrombin in real samples. In turn, the enriched thrombin attributed to the sensitive output of MALDI-TOF mass spectrometry signal, 0.085 ng μL(-1) (2.36 nM) thrombin could be detected. This proposed method has a relatively wide linear relation ranging from 0.1 ng μL(-1) to 10 ng μL(-1), and satisfactory specificity. The proposed high-throughput method based on MALDI-TOF MS is expected to the application in the disease biomarker detection and clinical diagnosis. PMID:25127596

  13. APPLICATION OF HPTLC-DENSITOMETRIC ANALYSIS FOR SIMULTANEOUS DETERMINATION OF CLIDINIUM BROMIDE, CHLORDIAZEPOXIDE AND PANTOPRAZOLE SODIUM IN THEIR COMBINED CAPSULE DOSAGE FORM

    Directory of Open Access Journals (Sweden)

    Dharati Rami

    2013-04-01

    Full Text Available A simple, precise, accurate and reliable HPTLC method has been developed and validated for analysis of Clidinium Bromide (CLBr, Pantoprazole Sodium (PNT and Chlordiazepoxide (CDZ in their combined dosage form. Identification and analysis were performed on 100mm x 100mm layer thickness 0.2 mm, pre-coated silica gel 60F254 aluminum sheet, prewashed with methanol and dried in an oven at 50°C for 5 min. Chloroform: Acetone: 0.5M Ammonium acetate in Methanol: Formic acid (1.0:5.0:3.0:1.0 (v/v/v/v was used as mobile phase. Calibration plots were established showing the dependence of response (peak area on the amount chromatographed. The validated calibration ranges were 600-1600 ng/spot, 4800-12800ng/spot and 1200-3200 ng/spot for CLBr, PNT and CDZ with correlation coefficient (R 2 0.998, 0.994 and 0.998 respectively. Average % recovery was between 99.63 – 99.89%, 99.87 – 100.05% and 99.77-99.93% for CLBr, PNT and CDZ respectively. The spots were scanned at 220nm in a reflectance mode. The proposed method has been validated as per ICH guidelines and successfully applied to the estimation of CLBr, PNT and CDZ in their combined Capsule dosage form.

  14. Analysis of PAHs Associated with Particulate Matter PM2.5 in Two Places at the City of Cuernavaca, Morelos, México

    Directory of Open Access Journals (Sweden)

    Hugo Saldarriaga-Noreña

    2015-08-01

    Full Text Available This study was carried out between January and February 2013, at two sites in the city of Cuernavaca, México, using low-volume equipment. Fifteen Polycyclic aromatic hydrocarbons (PAHs, were identified by gas chromatography coupled with mass spectrometry. The total average concentration observed for PAHs was 24.0 ng·m−3, with the high molecular weight compounds being the most abundant. The estimated equivalent concentration for Benzo (a P (BaPE was 4.05 ng·m−3. Diagnostic ratios together with the principal components analysis (PCA allowed for establishing coal burning and vehicle emissions as being the main sources of these compounds in the area. The PAHs used to calculate this index account for 51% of the 15 PAHs identified, which probably involves a risk to the exposed population.

  15. Multiresidue analysis of 88 polar organic micropollutants in ground, surface and wastewater using online mixed-bed multilayer solid-phase extraction coupled to high performance liquid chromatography-tandem mass spectrometry.

    Science.gov (United States)

    Huntscha, Sebastian; Singer, Heinz P; McArdell, Christa S; Frank, Carolin E; Hollender, Juliane

    2012-12-14

    An automated multiresidue method consisting of an online solid-phase extraction step coupled to a high performance liquid chromatography-tandem mass spectrometer (online-SPE-HPLC-MS/MS method) was developed for the determination of 88 polar organic micropollutants with a broad range of physicochemical properties (logD(OW) (pH 7): -4.2 to 4.2). Based on theoretical considerations, a single mixed-bed multilayer cartridge containing four different extraction materials was composed for the automated enrichment of water samples. This allowed the simultaneous analysis of pesticides, biocides, pharmaceuticals, corrosion inhibitors, many of their transformation products, and the artificial sweetener sucralose in three matrices groundwater, surface water, and wastewater. Limits of quantification (LOQs) were in the environmentally relevant concentration range of 0.1-87 ng/L for groundwater and surface water, and 1.5-206 ng/L for wastewater. The majority of the compounds could be quantified below 10 ng/L in groundwater (82%) and surface water (80%) and below 100 ng/L in wastewater (80%). Relative recoveries were largely between 80 and 120%. Intraday and inter-day precision, expressed as relative standard deviation, were generally better than 10% and 20%, respectively. 50 isotope labeled internal standards were used for quantification and accordingly, relative recoveries as well as intraday and inter-day precision were better for compounds with corresponding internal standard. The applicability of this method was shown during a sampling campaign at a riverbank filtration site for drinking water production with travel times of up to 5 days. 36 substances of all compound classes investigated could be found in concentrations between 0.1 and 600 ng/L. The results revealed the persistence of carbamazepine and sucralose in the groundwater aquifer as well as degradation of the metamizole metabolite 4-acetamidoantipyrine. PMID:23137864

  16. Multiresidue Analysis of Pesticides in Straw Roughage by Liquid Chromatography-Tandem Mass Spectrometry.

    Science.gov (United States)

    Zhang, Zihao; Feng, Mengyuan; Zhu, Kechen; Han, Lijun; Sapozhnikova, Yelena; Lehotay, Steven J

    2016-08-10

    A multiresidue analytical method using a modification of the "quick, easy, cheap, effective, rugged, and safe" (QuEChERS) sample preparation approach combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis was established and validated for the rapid determination of 69 pesticides at different levels (1-100 ng/g) in wheat and rice straws. In the quantitative analysis, the recoveries ranged from 70 to 120%, and consistent RSDs ≤ 20% were achieved for most of the target analytes (53 pesticides in wheat straw and 58 in rice straw). Almost all of the analytes achieved good linearity with R(2) > 0.98, and the limit of validation levels (LVLs) for diverse pesticides ranged from 1 to 10 ng/g. Different extraction and cleanup conditions were evaluated in both types of straw, leading to different options. The use of 0.1% formic acid or not in extraction with acetonitrile yielded similar final outcomes, but led to the use of a different sorbent in dispersive solid-phase extraction. Both options are efficient and useful for the multiresidue analysis of targeted pesticides in wheat and rice straw samples. PMID:26881844

  17. Establishment of Exposure to Organophosphorus Warfare Agents by Means of SPME-GSMS Analysis of Bodily Fluids

    International Nuclear Information System (INIS)

    Reliable chemical analytical procedures for revealing an exposure to toxic chemicals, identifying the active substance, and assessing the degree of exposure are necessary as a component of medical and forensic activities in cases of the possible use of highly toxic chemicals in war conflicts and terrorism acts, as well as emergency situations in chemical industry, specifically at chemical weapons storage and destruction facilities. According to Chemical Weapons Convention, Part XI, Appendix 4, e-17, 'samples of importance in the investigation of alleged use include biomedical samples from human or animal sources (blood, urine, excreta, tissue etc.)'. Urinary metabolites, O-alkyl esters of methylphosphic acid, offer one of the simplest means of confirming an exposure to organophosphorus warfare agents (OPWA). Urine, unlike blood or tissues, does not require invasive collection demanding in terms of sterility. Excretion with urine is the major route of elimination of OPWA from an organism. According to published data, 90% of OPWA metabolites are excreted within 48-72 h after intoxication. We developed an SPME-GCMS procedure for the determination of O-alkyl esters methylphosphonic acid in urine, with the following detection limits,: isopropyl and isobutyl esters 5 ng/ml and pinacolyl ester 1 ng/ml. The procedure involves derivatization of the target compounds directly on the microfiber. The total analysis time is 1-1.5 h. In animal experiments in vivo we could establish the exposure to OPWA at a half-LD50 level within no less than 48 h after intoxication. In principle, OPWA metabolites could be detected in urine within two weeks after intoxication but at higher doses. Retrospective analysis of urinary metabolites in cases of the exposure to low doses of OPWA requires lower detection limits (0.1-1 ng/ml). Optimal objects for the retrospective analysis of OPWA in an organism are long-lived blood protein adducts. We developed a procedure for revealing an exposure to

  18. Monoclonal Antibody-Based Enzyme Linked Immunosorbent Assay for the Analysis of Jasmonates in Plants

    Institute of Scientific and Technical Information of China (English)

    Aixing Deng; Weiming Tan; Suping He; Wei Liu; Tiegui Nan; Zhaohu Li; Baomin Wang; Qing X.Li

    2008-01-01

    Methyl jasmonate (MeJA) and its free-acid form,jasmonic acid (JA) are naturally occurring plant growth regulators widely distributed in higher plants.In order to improve the sensitivity for the analysis of MeJA at low levels in small amounts of plant samples,a monoclonal antibody (MAb) (designated as MAb 3E5D7C4B6) against MeJA was derived from a JAbovine serum albumin (BSA) conjugate as an immunogen.The antibody belongs to the IgG1 subclass with a κ type light chain and has a dissociation constant of approximately 6.07 x 10-9 M.MAb3E5D7C4B6 is very specific to MeJA.It was used to develop a direct competitive enzyme-linked immunosorbent assay (dcELISA),conventional and simplified indirect competitive ELISAs (icELISA).JA was derivatized into MeJA for the ELISA analysis.The IC50 value and detection range for MeJA were,respectively,34 and 4-257 ng/mL by the conventional icELISA,21 and 3-226 ng/mL by the simplified icELISA and 5.0 and 0.7-97.0 ng/mL by the dcELISA.The dcELISA was more sensitive than either the conventional or simplified icELISA.The assays were used to measure the content of jasmonates as MeJA in tobacco leaves under drought stress or inoculated with tobacco mosaic virus and tomato leaves inoculated with tomato mosaic virus or Lirioinyza sativae Blanchard as compared with the corresponding healthy leaves.The increased jasmonates content indicated its role in response to the drought stress and pathogens.

  19. Hair analysis to monitor abuse of analgesic combinations containing butalbital and propyphenazone.

    Science.gov (United States)

    Ferrari, Anna; Tiraferri, Ilaria; Palazzoli, Federica; Verri, Patrizia; Vandelli, Daniele; Marchesi, Filippo; Ciccarese, Michela; Licata, Manuela

    2015-11-10

    Butalbital, a barbiturate, is present in analgesic combinations used by headache sufferers. Overuse/abuse of these combinations may cause dependence, chronic migraine, and medication-overuse headache (MOH). MOH is difficult to manage: it improves interrupting analgesic overuse, but requires monitoring, because relapses are frequent. A gas chromatography-mass spectrometry (GC-MS) method for hair analysis has been developed and validated to document abuse of an analgesic combination containing butalbital and propyphenazone by a patient with MOH. For over ten years the patient managed her headache using eight suppositories/day of an analgesic combination containing butalbital 150mg, caffeine 75mg, and propyphenazone 375mg per suppository. An outpatient detoxification treatment was carried out. After three weeks, the patient reduced the consumption to one suppository/day. At the first control visit, after three months from the beginning of detoxification, the patient increased the use of the combination to four suppositories/day and at the second control visit, after seven months from the beginning of detoxification, she was back to eight suppositories/day. At the two control visits, a hair sample was taken for determination of butalbital and propyphenazone. Moreover blood and urine samples for determination of butalbital were drawn at the beginning of detoxification treatment and at the two control visits. With the segmental analysis of two hair samples the medication history of ten months could be estimated. In the first hair sample, collected at the first control visit, in the distal segment, butalbital and propyphenazone concentrations were, respectively, 17.5ng/mg and 56.0ng/mg, confirming the prolonged abuse; in the proximal segment, concurrently with the detoxification treatment, butalbital and propyphenazone concentrations had reduced respectively to 5.45ng/mg and 11.1ng/mg. The second hair sample, collected at the second control visit, proved the fair course

  20. Tramadol chronic abuse: an evidence from hair analysis by LC tandem MS.

    Science.gov (United States)

    Verri, Patrizia; Rustichelli, Cecilia; Palazzoli, Federica; Vandelli, Daniele; Marchesi, Filippo; Ferrari, Anna; Licata, Manuela

    2015-01-01

    Hair analysis, as complementary matrix, has expanded across the spectrum of toxicological investigations for misuse drug monitoring. Hair has become an important matrix for drug analysis, owing to the possibility to detect target analytes for long time periods, depending on hair length. A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method has been developed for the quantitation of tramadol, a widely used centrally acting analgesic, and its main metabolites in hair (ODMT, NDMT, NOT). Hair samples were decontaminated and incubated overnight in diluted hydrochloric acid; the extracts were purified by mixed-mode solid phase cartridges and analyzed by LC-MS/MS in positive ionization mode monitoring two transitions per analyte. The procedure was fully validated in terms of linearity, limit of detection and lower limit of quantitation (LLOQ), accuracy, precision, recovery, matrix effect and selectivity. The linear regression analysis was calibrated by deuterated internal standards; for all analytes, responses were linear over the range 0.04-40.00 ng/mg hair, with R(2) values of at least 0.995. The method offered satisfactory precision (RSD 90%) values. The found LLOQ values for tramadol and metabolites were in the range 0.010-0.030 ng/mg hair. The proposed procedure was successfully applied to quantify tramadol and metabolites in real hair samples submitted to our laboratory: three cases of tramadol assumption within the therapeutic dosage (3 × 2 segments) and one case of tramadol abuse in a binge pattern (8 segments). The ranges found for TRAM, ODMT, NDMT and NOT were markedly higher in the abuse case (63.42-107.30, 3.76-6.26, 24.88-45.66, 0.22-1.18 ng/mg hair, respectively) compared to the other case reports (3.29-20.12, 0.28-1.87, 0.45-4.32, 0.07-0.80 ng/mg, respectively); also the values of NMDT/ODMT ratio differed significantly. According to the obtained data, we hypothesized that the binge pattern may influence the metabolites' to parent drug

  1. Hair analysis to monitor abuse of analgesic combinations containing butalbital and propyphenazone.

    Science.gov (United States)

    Ferrari, Anna; Tiraferri, Ilaria; Palazzoli, Federica; Verri, Patrizia; Vandelli, Daniele; Marchesi, Filippo; Ciccarese, Michela; Licata, Manuela

    2015-11-10

    Butalbital, a barbiturate, is present in analgesic combinations used by headache sufferers. Overuse/abuse of these combinations may cause dependence, chronic migraine, and medication-overuse headache (MOH). MOH is difficult to manage: it improves interrupting analgesic overuse, but requires monitoring, because relapses are frequent. A gas chromatography-mass spectrometry (GC-MS) method for hair analysis has been developed and validated to document abuse of an analgesic combination containing butalbital and propyphenazone by a patient with MOH. For over ten years the patient managed her headache using eight suppositories/day of an analgesic combination containing butalbital 150mg, caffeine 75mg, and propyphenazone 375mg per suppository. An outpatient detoxification treatment was carried out. After three weeks, the patient reduced the consumption to one suppository/day. At the first control visit, after three months from the beginning of detoxification, the patient increased the use of the combination to four suppositories/day and at the second control visit, after seven months from the beginning of detoxification, she was back to eight suppositories/day. At the two control visits, a hair sample was taken for determination of butalbital and propyphenazone. Moreover blood and urine samples for determination of butalbital were drawn at the beginning of detoxification treatment and at the two control visits. With the segmental analysis of two hair samples the medication history of ten months could be estimated. In the first hair sample, collected at the first control visit, in the distal segment, butalbital and propyphenazone concentrations were, respectively, 17.5ng/mg and 56.0ng/mg, confirming the prolonged abuse; in the proximal segment, concurrently with the detoxification treatment, butalbital and propyphenazone concentrations had reduced respectively to 5.45ng/mg and 11.1ng/mg. The second hair sample, collected at the second control visit, proved the fair course

  2. Determination of propoxur in environmental samples by automated solid-phase extraction followed by flow-injection analysis with tris(2,2'-bipyridyl)ruthenium(II) chemiluminescence detection

    Energy Technology Data Exchange (ETDEWEB)

    Perez-Ruiz, Tomas [Department of Analytical Chemistry, Faculty of Chemistry, University of Murcia, 30071 Murcia (Spain)]. E-mail: tpr@um.es; Martinez-Lozano, Carmen [Department of Analytical Chemistry, Faculty of Chemistry, University of Murcia, 30071 Murcia (Spain); Garcia, Maria Dolores [Department of Analytical Chemistry, Faculty of Chemistry, University of Murcia, 30071 Murcia (Spain)

    2007-02-19

    A sensitive method for the analysis of propoxur in environmental samples has been developed. It involves an automated solid-phase extraction (SPE) procedure using a Gilson Aspec XLi and flow-injection analysis (FI) with chemiluminescence (CL) detection. The FI-CL system relies on the photolysis of propoxur by irradiation using a low-pressure mercury lamp (main spectral line 254 nm). The resultant methylamine is subsequently detected by CL using tris(2,2'-bipyridyl)ruthenium(III), which is on-line generated by photo-oxidation of the ruthenium(II) complex in the presence of peroxydisulfate. The linear concentration range of application was 0.05-5 {mu}g mL{sup -1} of propoxur, with a detection limit of 5 ng mL{sup -1}. The repeatability was 0.82% expressed as relative standard deviation (n = 10) and the reproducibility, studied on 5 consecutive days, was 2.1%. The sample throughput was 160 injection per hour. Propoxur residues below ng mL{sup -1} levels could be determined in environmental water samples when an SPE preconcentration device was coupled on-line with the FI system. This SPE-FI-CL arrangement provides a detection limit as low as 5 ng L{sup -1} using only 500 mL of sample. In the analysis of fruits and vegetables, the detection limit was about 10 {mu}g kg{sup -1}.

  3. Determination of propoxur in environmental samples by automated solid-phase extraction followed by flow-injection analysis with tris(2,2'-bipyridyl)ruthenium(II) chemiluminescence detection.

    Science.gov (United States)

    Pérez-Ruiz, Tomás; Martínez-Lozano, Carmen; García, María Dolores

    2007-02-19

    A sensitive method for the analysis of propoxur in environmental samples has been developed. It involves an automated solid-phase extraction (SPE) procedure using a Gilson Aspec XLi and flow-injection analysis (FI) with chemiluminescence (CL) detection. The FI-CL system relies on the photolysis of propoxur by irradiation using a low-pressure mercury lamp (main spectral line 254 nm). The resultant methylamine is subsequently detected by CL using tris(2,2'-bipyridyl)ruthenium(III), which is on-line generated by photo-oxidation of the ruthenium(II) complex in the presence of peroxydisulfate. The linear concentration range of application was 0.05-5 microg mL(-1) of propoxur, with a detection limit of 5 ng mL(-1). The repeatability was 0.82% expressed as relative standard deviation (n=10) and the reproducibility, studied on 5 consecutive days, was 2.1%. The sample throughput was 160 injection per hour. Propoxur residues below ng mL(-1) levels could be determined in environmental water samples when an SPE preconcentration device was coupled on-line with the FI system. This SPE-FI-CL arrangement provides a detection limit as low as 5 ng L(-1) using only 500 mL of sample. In the analysis of fruits and vegetables, the detection limit was about 10 microg kg(-1).

  4. Cobalt internal standard for Ni to assist the simultaneous determination of Mo and Ni in plant materials by high-resolution continuum source graphite furnace atomic absorption spectrometry employing direct solid sample analysis.

    Science.gov (United States)

    de Babos, Diego Victor; Bechlin, Marcos André; Barros, Ariane Isis; Ferreira, Edilene Cristina; Gomes Neto, José Anchieta; de Oliveira, Silvana Ruella

    2016-05-15

    A new method is proposed for the simultaneous determination of Mo and Ni in plant materials by high-resolution continuum source graphite furnace atomic absorption spectrometry (HR-CS GFAAS), employing direct solid sample analysis (DSS) and internal standardization (IS). Cobalt was used as internal standard to minimize matrix effects during Ni determinations, enabling the use of aqueous standards for calibration. Correlation coefficients for the calibration curves were typically better than 0.9937. The performance of the method was checked by analysis of six plant certified reference materials, and the results for Mo and Ni were in agreement with the certified values (95% confidence level, t-test). Analysis was made of different types of plant materials used as renewable sources of energy, including sugarcane leaves, banana tree fiber, soybean straw, coffee pods, orange bagasse, peanut hulls, and sugarcane bagasse. The concentrations found for Mo and Ni ranged from 0.08 to 0.63 ng mg(-1) and from 0.41 to 6.92 ng mg(-1), respectively. Precision (RSD) varied from 2.1% to 11% for Mo and from 3.7% to 10% for Ni. Limits of quantification of 0.055 and 0.074 ng were obtained for Mo and Ni, respectively. PMID:26992542

  5. The Application of Voltammetric Analysis of Δ(9) -THC for the Reduction of False Positive Results in the Analysis of Suspected Marijuana Plant Matter.

    Science.gov (United States)

    Balbino, Marco A; de Oliveira, Laura S; Eleotério, Izabel C; Oiye, Erica N; Ribeiro, Maria F M; McCord, Bruce R; Ipolito, Antonio J; de Oliveira, Marcelo F

    2016-07-01

    The development of methodologies using inexpensive, fast, and reliable instrumention is desirable in illicit drug analysis. The purpose of this study was based on cyclic voltammetry technique to differentiate the electrochemical behavior of ∆(9) -THC, the psychoactive substance in marijuana, and five different extract plants to yield false positive results after analysis protocol for cannabinoids using thin-layer chromatography and Fast Blue B salt. After applying a deposition potential of -0.5 V in a glassy carbon working electrode, the results indicated an anodic peak current at 0.0 V versus Ag/AgCl after addition of ∆(9) -THC solution in the electrochemical cell, and limits of detection and quantification were 1.0 ng mL(-1) and 3.5 ng mL(-1) , respectively. Other interfering plants showed distinct amperometric responses. This methodology was useful to detect ∆(9) -THC even in the presence of the Fast Blue B salt, which avoided false positive results for all the studied extract plants. PMID:27364289

  6. “Beyhude Ömrüm” Adlı Hikâyenin Greımas’ın Eyleyenler Modeline Göre İncelenmesi Analysıs Of Story “Beyhude Ömrüm” Accordıng To Greımas’ Actants Model

    Directory of Open Access Journals (Sweden)

    Hatice ALTUNKAYA

    2012-12-01

    Full Text Available The art of story is a form of narrative that is not independent from its creator, namely the narrator, and created within the theme fictionalized by its narrator. When the work is created, it is the reader’s turn to interpret and give meaning to it. The reader’s duty is to reinterpret, transform the structures in the text and themes as well as unveiling the meaning connections between those. Semiology derivedfrom Ancient Greek word semeion meaning sign and logos meaningscience is known as science of signs in Turkish.Two people should be mentioned as founders of Semiology. Theseare Charles Sanders Peirce and Swiss linguist Ferdinand de Saussure.Saying “Although the universe is not entirely made up of signs, itoverflows with signs”, Peirce expresses that all objects can be subject ofSemiology.The goal of this study is to analyze Mustafa Kutlu’s story titled“Beyhude Ömrüm” according to the Greimas’ analysis method ofactants model. A.J. Greimas argues that an incident starting the storyand an element transforming the initial status are required for creatingthe narrative. The study divided the story into five sections byconsidering that the said incident and transforming elements areavailable. The sections confirmed that six actants of the actants modelare available; manipulation, competence and performance of narrativeroutine as well as stages of sanction routine are available and thesubjects of actants model realized competence, conclusive andhonorable experiences. Besides, it is seen that using the expression “Idied” is not an appropriate form of narrative used by the author tellingthe story as a narrator using pronoun I. Hikâye sanatı, onu meydana getiren anlatıcısından bağımsız olmayan, anlatıcısının kurguladığı izlencede meydana gelen bir anlatı türüdür. Eser meydana geldikten sonra eseri yorumlama, anlamlandırma sırası okura gelir. Metnin içerdiği yapıları, izlekleri yeniden yorumlamak, d

  7. Instrumental analysis

    International Nuclear Information System (INIS)

    This textbook deals with instrumental analysis, which consists of nine chapters. It has Introduction of analysis chemistry, the process of analysis and types and form of the analysis, Electrochemistry on basic theory, potentiometry and conductometry, electromagnetic radiant rays and optical components on introduction and application, Ultraviolet rays and Visible spectrophotometry, Atomic absorption spectrophotometry on introduction, flame emission spectrometry and plasma emission spectrometry. The others like infrared spectrophotometry, X-rays spectrophotometry and mass spectrometry, chromatography and the other instrumental analysis like radiochemistry.

  8. Analysis of metals and phosphorus in biodiesel B100 from different feedstock using a Flow Blurring® multinebulizer in inductively coupled plasma-optical emission spectrometry

    Energy Technology Data Exchange (ETDEWEB)

    Avila Orozco, Francisco D. [Lab. FIA, Sección Química Analítica, INQUISUR (UNS-CONICET), Universidad Nacional del Sur, Avenida Alem 1253, B8000CPB, Bahía Blanca (Argentina); Kovachev, Nikolay; Aguirre Pastor, Miguel Ángel [Dpto. Química Analítica, Nutrición y Bromatología e Instituto Universitario de Materiales, Facultad de Ciencias, Universidad de Alicante, Apdo. 99, Alicante (Spain); Domini, Claudia E.; Fernández Band, Beatriz S. [Lab. FIA, Sección Química Analítica, INQUISUR (UNS-CONICET), Universidad Nacional del Sur, Avenida Alem 1253, B8000CPB, Bahía Blanca (Argentina); Canals Hernández, Antonio, E-mail: a.canals@ua.es [Dpto. Química Analítica, Nutrición y Bromatología e Instituto Universitario de Materiales, Facultad de Ciencias, Universidad de Alicante, Apdo. 99, Alicante (Spain)

    2014-05-01

    Highlights: • The elemental analysis of biodiesel by the proposed method is simple and fast. • Two-nozzles Flow Blurring® nebulizer allow to reduce the spectral interferences. • Two-nozzles Flow Blurring® nebulizer avoid the formation of carbon deposits. • The analysis may be carried out without any sample pretreatment. Abstract: A simple and fast method for determining the content of Na, K, Ca, Mg, P, and 20 heavy metals in biodiesel samples with inductively coupled plasma optical emission spectrometry (ICP OES) using a two-nozzle Flow Blurring® multinebulizer prototype and on-line internal standard calibration, are proposed. The biodiesel samples were produced from different feedstock such as sunflower, corn, soybean and grape seed oils, via a base catalyst transesterification. The analysis was carried out without any sample pretreatment. The standards and samples were introduced through one of the multinebulizer nozzles, while the aqueous solution containing yttrium as an internal standard was introduced through the second nozzle. Thus, the spectral interferences were compensated and the formation of carbon deposits on the ICP torch was prevented. The determination coefficients (R²) were greater than 0.99 for the studied analytes, in the range 0.21–14.75 mg kg⁻¹. Short-term and long-term precisions were estimated as relative standard deviation. These were acceptable, their values being lower than 10%. The LOQ for major components such as Ca, K, Mg, Na, and P, were within a range between 4.9 ng g⁻¹ for Mg (279.553 nm) and 531.1 ng g⁻¹ for Na (588.995 nm), and for the other 20 minor components they were within a range between 1.1 ng g⁻¹ for Ba (455.403 nm) and 2913.9 ng g⁻¹ for Pb (220.353 nm). Recovery values ranged between 95% and 106%.

  9. Optimizing sample pretreatment for compound-specific stable carbon isotopic analysis of amino sugars in marine sediment

    Directory of Open Access Journals (Sweden)

    R. Zhu

    2014-01-01

    Full Text Available Amino sugars are quantitatively significant constituents of soil and marine sediment, but their sources and turnover in environmental samples remain poorly understood. The stable carbon isotopic composition of amino sugars can provide information on the lifestyles of their source organisms and can be monitored during incubations with labeled substrates to estimate the turnover rates of microbial populations. However, until now, such investigation has been carried out only with soil samples, partly because of the much lower abundance of amino sugars in marine environments. We therefore optimized a procedure for compound-specific isotopic analysis of amino sugars in marine sediment employing gas chromatography-isotope ratio mass spectrometry. The whole procedure consisted of hydrolysis, neutralization, enrichment, and derivatization of amino sugars. Except for the derivatization step, the protocol introduced negligible isotopic fractionation, and the minimum requirement of amino sugar for isotopic analysis was 20 ng, i.e. equivalent to ~ 8 ng of amino sugar carbon. Our results obtained from δ13C analysis of amino sugars in selected marine sediment samples showed that muramic acid had isotopic imprints from indigenous bacterial activities, whereas glucosamine and galactosamine were mainly derived from organic detritus. The analysis of stable carbon isotopic compositions of amino sugars opens a promising window for the investigation of microbial metabolisms in marine sediments and the deep marine biosphere.

  10. Supported liquid extraction (SLE) for the analysis of methylamphetamine, methylenedioxymethylamphetamine and delta-9-tetrahydrocannabinol in oral fluid and blood of drivers.

    Science.gov (United States)

    Rositano, Joanna; Harpas, Peter; Kostakis, Chris; Scott, Timothy

    2016-08-01

    Since 2006, the South Australian Government has been conducting roadside oral fluid testing of drivers for the illicit drugs methylamphetamine (MA), methylenedioxymethylamphetamine (MDMA) and Δ(9)-tetrahydrocannabinol (THC) using the Securetec Drugwipe II Twin and Alere DDS 805 AP saliva collection kit. Forensic Science South Australia carries out the confirmatory analysis by LC/MS for the positive test results of oral fluid roadside testing along with the pre-screened ELISA positive road traffic accident blood samples. The number of blood and oral fluid samples received in the laboratory has been steadily increasing during this time, and over 10,000 samples were received in 2014. The proportion of positive results from these samples has also been increasing over the decade of driver drug testing, and this data is presented. A simple and efficient method has been developed for the analysis of the three drugs using Biotage Isolute(®) SLE+ 96-well plates. Sample preparation included 1:1 dilution with a dilute ammonia solution for buffered oral fluids (1:3 dilution for blood samples), and addition of deuterated internal standards. Samples were loaded onto the phase, left to absorb for 5min then eluted with methyl t-butyl ether (MTBE). The samples were evaporated and reconstituted in methanol. LC/MS analysis was performed on an AB Sciex 5500 Q-Trap in positive ion mode, monitoring 3 transitions for each analyte. Separation was achieved on a Restek Ultrabiphenyl 50×2.1mm column with a gradient system of acetonitrile/0.1% formic acid over 5min. Method validation and recoveries were carried out on drug free ante mortem blood and DDS buffer solution provided by Alere, Australia. Recoveries above 80% were achieved for MA and MDMA at a concentration of 25ng/mL, whilst recoveries of greater than 65% were achieved for THC at 4.5ng/mL. Accuracy and precision were acceptable down to the LLOQ for all three analytes (5, 5 and 1ng/mL for MA, MDMA and THC, respectively). Mean

  11. Double-ion imprinted polymer @magnetic nanoparticles modified screen printed carbon electrode for simultaneous analysis of cerium and gadolinium ions

    Energy Technology Data Exchange (ETDEWEB)

    Prasad, Bhim Bali, E-mail: prof.bbpd@yahoo.com; Jauhari, Darshika

    2015-05-22

    Highlights: • Synthesis of a double-ion imprinted polymer for analysis of Ce(IV) and Gd(III). • Imprinted nano-beads were grown on MNPs-modified SPCE surface. • Voltammetric determination of both templates was carried out simultaneously. • Ultra-trace analysis with LOD (ng mL{sup −1}) 0.07 for Ce(IV) and 0.19 for Gd(III) is achieved. - Abstract: A typical, reproducible, and rugged screen printed carbon electrode, modified with dual-ion imprinted beads, was fabricated employing the “surface grafting from” approach. For this, the acyl chloride functionalized magnetic nanoparticles were first immobilized and chemically attached with a typical functional monomer (but-2-enedioic acid bis-[(2-amino-ethyl)-amide]) on the electrode surface. This was subsequently subjected to the thermal polymerization in the presence of template ions (Ce(IV) and Gd(III)), cross-linker (ethylene glycol dimethacrylate), initiator (AIBN), and multiwalled carbon nanotubes. The modified sensor was used for the simultaneous analysis of both template ions in aqueous, blood serum, and waste-water samples, using differential pulse anodic stripping voltammetry which revealed two oxidation peaks for respective templates with resolution as much as 950 mV, without any cross reactivity, interferences and false-positives. The detection limits realized by the proposed sensor, under optimized conditions, were found to be as low as 0.07 ng mL{sup −1} for Ce(IV) and 0.19 ng mL{sup −1} for Gd(III) (S/N = 3) that could eventually be helpful for lanthanide estimation at stringent levels.

  12. Analysis of heterocyclic aromatic amines.

    Science.gov (United States)

    Murkovic, M

    2007-09-01

    Heterocyclic aromatic amines are formed in protein and amino acid-rich foods at temperatures above 150 degrees C. Of more than twenty heterocyclic aromatic amines identified ten have been shown to have carcinogenic potential. As nutritional hazards, their reliable determination in prepared food, their uptake and elimination in living organisms, including humans, and assessment of associated risks are important food-safety issues. The concentration in foods is normally in the low ng g(-1) range, which poses a challenge to the analytical chemist. Because of the complex nature of food matrixes, clean-up and enrichment of the extracts are also complex, usually involving both cation-exchange (propylsulfonic acid silica gel, PRS) and reversed-phase purification. The application of novel solid-phase extraction cartridges with a wettable apolar phase combined with cation-exchange characteristics simplified this process--both the polar and apolar heterocyclic aromatic amines were recovered in one fraction. Copper phthalocyanine trisulfonate bonded to cotton ("blue cotton") or rayon, and molecular imprinted polymers have also been successfully used for one-step sample clean-up. For analysis of the heterocyclic aromatic amines, liquid chromatography with base-deactivated reversed-phase columns has been used, and, recently, semi-micro and capillary columns have been introduced. The photometric, fluorimetric, or electrochemical detectors used previously have been replaced by mass spectrometers. Increased specificity and sub-ppb sensitivities have been achieved by the use of the selected-reaction-monitoring mode of detection of advanced MS instrumentation, for example the triple quadrupole and Q-TOF instrument combination. Gas chromatography, also with mass-selective detection, has been used for specific applications; the extra derivatization step needed for volatilization has been balanced by the higher chromatographic resolution. PMID:17546447

  13. Waste-to-wheel analysis of anaerobic-digestion-based renewable natural gas pathways with the GREET model.

    Energy Technology Data Exchange (ETDEWEB)

    Han, J.; Mintz, M.; Wang, M. (Energy Systems)

    2011-12-14

    In 2009, manure management accounted for 2,356 Gg or 107 billion standard cubic ft of methane (CH{sub 4}) emissions in the United States, equivalent to 0.5% of U.S. natural gas (NG) consumption. Owing to the high global warming potential of methane, capturing and utilizing this methane source could reduce greenhouse gas (GHG) emissions. The extent of that reduction depends on several factors - most notably, how much of this manure-based methane can be captured, how much GHG is produced in the course of converting it to vehicular fuel, and how much GHG was produced by the fossil fuel it might displace. A life-cycle analysis was conducted to quantify these factors and, in so doing, assess the impact of converting methane from animal manure into renewable NG (RNG) and utilizing the gas in vehicles. Several manure-based RNG pathways were characterized in the GREET (Greenhouse gases, Regulated Emissions, and Energy use in Transportation) model, and their fuel-cycle energy use and GHG emissions were compared to petroleum-based pathways as well as to conventional fossil NG pathways. Results show that despite increased total energy use, both fossil fuel use and GHG emissions decline for most RNG pathways as compared with fossil NG and petroleum. However, GHG emissions for RNG pathways are highly dependent on the specifics of the reference case, as well as on the process energy emissions and methane conversion factors assumed for the RNG pathways. The most critical factors are the share of flared controllable CH{sub 4} and the quantity of CH{sub 4} lost during NG extraction in the reference case, the magnitude of N{sub 2}O lost in the anaerobic digestion (AD) process and in AD residue, and the amount of carbon sequestered in AD residue. In many cases, data for these parameters are limited and uncertain. Therefore, more research is needed to gain a better understanding of the range and magnitude of environmental benefits from converting animal manure to RNG via AD.

  14. Gut fluorescence analysis of barnacle larvae: An approach to quantify the ingested food

    Digital Repository Service at National Institute of Oceanography (India)

    Gaonkar, C.A.; Anil, A.C.

    pellets it was 224(plus or minus 63) ng chlorophyll a larva sup(−1). A phaeopigment concentration in larval gut was found to be 311(plus or minus 13) ng larva sup(−1) and in faecal pellets it was 172(plus or minus 61) ng larva sup(−1). The study also...

  15. Analysis of furan in coffee of different provenance by head-space solid phase microextraction gas chromatography-mass spectrometry: effect of brewing procedures.

    Science.gov (United States)

    La Pera, Lara; Liberatore, Alfredo; Avellone, Giuseppe; Fanara, Serena; Dugo, Giacomo; Agozzino, Pasquale

    2009-06-01

    A simple, sensitive and accurate method for the analysis of furan in roasted coffee has been used based on headspace-solid-phase micro-extraction (HS-SPME) coupled to gas chromatography-mass spectrometry (GC-MS). The extraction was performed using 75-microm carboxen/polydimethylsiloxane fiber. Ionic strength, extraction time and temperature, and desorption time were assessed as the most important parameters affecting the HS-SPME procedure and d(4)-furan was used as the internal standard. The linearity range was in the range 0.0075-0.486 ng g(-1); the LOD and LOQ calculated using the signal-to-noise ratio approach were 0.002 and 0.006 ng g(-1), respectively. The inter- and intra-day precision was 8 and 10%, respectively. The concentration of furan found in batches of roasted coffee powder different producing countries ranged from 57.3 to 587.3 ng g(-1). The mean reduction in furan levels observed when brewing coffee by either infusion, using a moka pot or an expresso machine was 57, 67.5 and 63.3%, respectively. PMID:19680951

  16. NAA and low-background Υ- spectrometry for the analysis of U and Th in high purity silica used in electronic devices

    International Nuclear Information System (INIS)

    It has been established that soft error of high precision electronic circuits can be induced by alpha particle emitted from the naturally occurring radioactive impurities such as U and Th. Silica has used in electronic industries as the base materials and impurities must be controlled strictly. Recently, electronic circuits have become lower dimension and higher density, so alpha-particle emitting radioactive impurities have to be strictly controlled. The objective of the present study was to develop of NAA and gamma-spectrometry for the sensitive (ng/g) and precise analysis of U and Th. A new NAA method has been established using the HTS irradiation facility which has been used to produce radioisotopes for industries and medicines. When the ultratrace impurities have to be analyzed by NAA, background gamma-ray spectra induced from 222Rn and its progeny nuclides in atmosphere are serious problem. This unstable background was eliminated and stabilized by the use of a nitrogen purging system. By the use of HTS-NAA and low background gamma-spectrometry ultratrace amounts of U (0.1ng/g) and Th (0.01ng/g) in high purity silica could be analyzed

  17. Whole air canister sampling coupled with preconcentration GC/MS analysis of part-per-trillion levels of trimethylsilanol in semiconductor cleanroom air.

    Science.gov (United States)

    Herrington, Jason S

    2013-08-20

    The costly damage airborne trimethylsilanol (TMS) exacts on optics in the semiconductor industry has resulted in the demand for accurate and reliable methods for measuring TMS at trace levels (i.e., parts per trillion, volume per volume of air [ppt(v)] [~ng/m(3)]). In this study I developed a whole air canister-based approach for field sampling trimethylsilanol in air, as well as a preconcentration gas chromatography/mass spectrometry laboratory method for analysis. The results demonstrate clean canister blanks (0.06 ppt(v) [0.24 ng/m(3)], which is below the detection limit), excellent linearity (a calibration relative response factor relative standard deviation [RSD] of 9.8%) over a wide dynamic mass range (1-100 ppt(v)), recovery/accuracy of 93%, a low selected ion monitoring method detection limit of 0.12 ppt(v) (0.48 ng/m(3)), replicate precision of 6.8% RSD, and stability (84% recovery) out to four days of storage at room temperature. Samples collected at two silicon wafer fabrication facilities ranged from 10.0 to 9120 ppt(v) TMS and appear to be associated with the use of hexamethyldisilazane priming agent. This method will enable semiconductor cleanroom managers to monitor and control for trace levels of trimethylsilanol.

  18. Vitamin D supplementation during rehabilitation in COPD: a secondary analysis of a randomized trial

    Directory of Open Access Journals (Sweden)

    Hornikx Miek

    2012-09-01

    Full Text Available Abstract Rationale Pulmonary rehabilitation is an important treatment for patients with Chronic Obstructive Pulmonary Disease, who are often vitamin D deficient. As vitamin D status is linked to skeletal muscle function, we aimed to explore if high dose vitamin D supplementation can improve the outcomes of rehabilitation in Chronic Obstructive Pulmonary Disease. Material and methods This study is a post-hoc subgroup analysis of a larger randomized trial comparing a monthly dose of 100.000 IU of vitamin D with placebo to reduce exacerbations. 50 Subjects who followed a rehabilitation program during the trial are included in this analysis. We report changes from baseline in muscle strength and exercise performance between both study arms after 3 months of rehabilitation. Results Vitamin D intervention resulted in significantly higher median vitamin D levels compared to placebo (51 [44-62] ng/ml vs 15 [13-30] ng/ml; p 0.050. Conclusion High dose vitamin D supplementation during rehabilitation may have mild additional benefits to training.

  19. Nanogram-scale preparation and NMR analysis for mass-limited small volatile compounds.

    Directory of Open Access Journals (Sweden)

    Satoshi Nojima

    Full Text Available Semiochemicals are often produced in infinitesimally small quantities, so their isolation requires large amounts of starting material, not only requiring significant effort in sample preparation, but also resulting in a complex mixture of compounds from which the bioactive compound needs to be purified and identified. Often, compounds cannot be unambiguously identified by their mass spectra alone, and NMR analysis is required for absolute chemical identification, further exacerbating the situation because NMR is relatively insensitive and requires large amounts of pure analyte, generally more than several micrograms. We developed an integrated approach for purification and NMR analysis of <1 µg of material. Collections from high performance preparative gas-chromatography are directly eluted with minimal NMR solvent into capillary NMR tubes. With this technique, (1H-NMR spectra were obtained on 50 ng of geranyl acetate, which served as a model compound, and reasonable H-H COSY NMR spectra were obtained from 250 ng of geranyl acetate. This simple off-line integration of preparative GC and NMR will facilitate the purification and chemical identification of novel volatile compounds, such as insect pheromones and other semiochemicals, which occur in minute (sub-nanogram, and often limited, quantities.

  20. Quantification of fentanyl in serum by isotope dilution analysis using capillary gas chromatography

    Energy Technology Data Exchange (ETDEWEB)

    Sera, Shoji; Goromaru, Tsuyoshi [Fukuyama Univ., Hiroshima (Japan); Sameshima, Teruko; Kawasaki, Koichi; Oda, Toshiyuki

    1998-06-01

    The quantitative determination of fentanyl (FT) in serum was examined by isotope dilution analysis using a capillary gas chromatograph equipped with a surface ionization detector. The separation of FT and its deuterated analogue, FT-{sup 2}H{sub 19}, was achieved within 15 min a column temperature of 260degC by using a 25 m column. Measurement of the samples prepared by the addition of a known amount of FT in the range of 0.2 to 40 ng/ml with 20 ng/ml of FT-{sup 2}H{sub 19} to human control serum allowed observation of a linear relationship between the peak area ratio and the added amount ratio. The correlation coefficient obtained by regression analysis was 0.999. The advantage of the present isotope dilution method was demonstrated by comparison with other FT analogues which substituted a propionyl group with an acetyl group or a phenethyl group with a benzyl group as the internal standard. The present method was used to determine the serum level of FT in surgical patients after i.v. administration. No endogenous compounds and concomitant drugs interfered with the detection of FT or FT-{sup 2}H{sub 19}. This method was considered to be useful for the pharmacokinetic study of FT in patients. (author)