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Sample records for analysis tie-hua ng

  1. Analysis of Coexisting GPON and NG-PON1 (10G-PON Systems

    Directory of Open Access Journals (Sweden)

    M. D. Mraković

    2011-06-01

    Full Text Available In this paper, the simulation model of coexisting GPON and NG-PON1 (10G-PON systems is presented, which has been developed for the analysis of feasibility and implementation issues of this coexistence. The aim was to analyze the impact of the most important parameters of the components that are needed for new network elements, on the performance of these coexistent networks. On the basis of the results obtained, the optimal parameters of the new system components were defined.

  2. Nanoscopic strength analysis of work-hardened L-grade austenitic stainless steel, 316(NG)

    International Nuclear Information System (INIS)

    Stress corrosion cracking (SCC) occurs in shrouds and piping of L-grade austenitic stainless steels at nuclear power plants. A work-hardened layer, where the transgranular SCC initiates, is considered to be one of the probable cause for this occurrence. In order to clarify the microstructural characteristics of work-hardened layer at the surface of shrouds or piping, the strengthen analysis of L-grade austenitic stainless steel, 316(NG), rolled at the reduction in area, RA, of 10, 20, 30, 40 and 50% at room temperature were conducted on a nanoscopic scale, using an ultra-microhardness tester, TEM and SEM. TEM and SEM observation showed that the microstructural parameters are the dislocation cell size, dcel, coarse slip spacing, lcsl, and austenitic grain size, dγ. Referring 10dcel and 10lcsl, Vickers hardness, Hυ, corresponding to macro strength was expressed as Hυ = Hυ*bas + Hυ*sol + Hυ*dis + Hυ*cel + Hυ*csl. Hυ*bas(=100) is the base hardness, Hυ*sol is the solid solution strengthening hardness, Hυ*dis is the dislocation strengthening hardness in the dislocation cell, and Hυ*cel and Hυ*csl are the fine grain strengthening hardness due to the dislocation cell and coarse slip. Hυ*sol was about 50, independently of RA. Hυ*dis was zero at RA30%. Hυ*cel and Hυ*csl increased with increasing in RA and were kept constant at about 50 and 120 at RA=20 and 30%, respectively. It was suggested from these results that all dislocations introduced by rolling might be dissipated for the creation of dislocation cells and coarse slips at RA30%. (author)

  3. NG09 And CTBT On-Site Inspection Noble Gas Sampling and Analysis Requirements

    Science.gov (United States)

    Carrigan, Charles R.; Tanaka, Junichi

    2010-05-01

    A provision of the Comprehensive Test Ban Treaty (CTBT) allows on-site inspections (OSIs) of suspect nuclear sites to determine if the occurrence of a detected event is nuclear in origin. For an underground nuclear explosion (UNE), the potential success of an OSI depends significantly on the containment scenario of the alleged event as well as the application of air and soil-gas radionuclide sampling techniques in a manner that takes into account both the suspect site geology and the gas transport physics. UNE scenarios may be broadly divided into categories involving the level of containment. The simplest to detect is a UNE that vents a significant portion of its radionuclide inventory and is readily detectable at distance by the International Monitoring System (IMS). The most well contained subsurface events will only be detectable during an OSI. In such cases, 37 Ar and radioactive xenon cavity gases may reach the surface through either "micro-seepage" or the barometric pumping process and only the careful siting of sampling locations, timing of sampling and application of the most site-appropriate atmospheric and soil-gas capturing methods will result in a confirmatory signal. The OSI noble gas field tests NG09 was recently held in Stupava, Slovakia to consider, in addition to other field sampling and analysis techniques, drilling and subsurface noble gas extraction methods that might be applied during an OSI. One of the experiments focused on challenges to soil-gas sampling near the soil-atmosphere interface. During withdrawal of soil gas from shallow, subsurface sample points, atmospheric dilution of the sample and the potential for introduction of unwanted atmospheric gases were considered. Tests were designed to evaluate surface infiltration and the ability of inflatable well-packers to seal out atmospheric gases during sample acquisition. We discuss these tests along with some model-based predictions regarding infiltration under different near

  4. Analysis of axonal regeneration in the central and peripheral nervous systems of the NG2-deficient mouse

    Directory of Open Access Journals (Sweden)

    Lieberman Alexander R

    2007-09-01

    Full Text Available Abstract Background The chondroitin sulphate proteoglycan NG2 blocks neurite outgrowth in vitro and has been proposed as a major inhibitor of axonal regeneration in the CNS. Although a substantial body of evidence underpins this hypothesis, it is challenged by recent findings including strong expression of NG2 in regenerating peripheral nerve. Results We studied axonal regeneration in the PNS and CNS of genetically engineered mice that do not express NG2, and in sex and age matched wild-type controls. In the CNS, we used anterograde tracing with BDA to study corticospinal tract (CST axons after spinal cord injury and transganglionic labelling with CT-HRP to trace ascending sensory dorsal column (DC axons after DC lesions and a conditioning lesion of the sciatic nerve. Injury to these fibre tracts resulted in no difference between knockout and wild-type mice in the ability of CST axons or DC axons to enter or cross the lesion site. Similarly, after dorsal root injury (with conditioning lesion, most regenerating dorsal root axons failed to grow across the dorsal root entry zone in both transgenic and wild-type mice. Following sciatic nerve injuries, functional recovery was assessed by analysis of the toe-spreading reflex and cutaneous sensitivity to Von Frey hairs. Anatomical correlates of regeneration were assessed by: retrograde labelling of regenerating dorsal root ganglion (DRG cells with DiAsp; immunostaining with PGP 9.5 to visualise sensory reinnervation of plantar hindpaws; electron microscopic analysis of regenerating axons in tibial and digital nerves; and by silver-cholinesterase histochemical study of motor end plate reinnervation. We also examined functional and anatomical correlates of regeneration after injury of the facial nerve by assessing the time taken for whisker movements and corneal reflexes to recover and by retrograde labelling of regenerated axons with Fluorogold and DiAsp. None of the anatomical or functional analyses

  5. Determination of iridium at low levels (sub ng g-1) in geological materials by neutron activation analysis

    International Nuclear Information System (INIS)

    The analysis of the platinum group elements (PGE: Ru, Rh, Pd, Os, Ir and Pt) in geological materials is difficult, due to the low concentrations of these elements (ng g-1 or sub ng g-1) and their heterogeneous distribution in many geological matrices. The determination of PGE has attracted great interest due not only to the increasing utilization of these elements in modern industry, but also to the information that these elements can provide on mantle processes. The determination of very low amounts of iridium is particularly important on account of some anomalous concentrations of iridium in sedimentary rock samples, related to the impact of an extraterrestrial object responsible for extinctions at the Cretaceous-Tertiary (K-T) boundary. In the present paper, a radiochemical neutron activation method for the determination of iridium in geological materials is presented. The procedure consisted of thermal neutron irradiation of about 500 mg of the sample, followed by sintering with sodium peroxide, precipitation with tellurium and high resolution gamma-ray spectrometry with a hyper-pure Ge detector. The accuracy and precision of the procedure were evaluated by analysis of the certified reference material SARM-7 (South Africa Bureau of Standards) and W-1 (USGS). The detection limit for the analytical conditions employed was 0.004 ng g-1. The procedure was applied to the reference materials TDB-1 and WGB-1 (CANMET), which present provisional values for Ir, and to the reference materials GXR-3, GXR-5 and GXR- 6 (USGS), which do not present information values for Ir. This work is a contribution to Ir values in these reference materials. As an example of application of the method to real samples, the developed procedure was employed in the determination of iridium in basalts from Parana basin, collected in Bom Guara do Sul, Santa Catarina, provided by the Geosciences Institute of the University of Campinas. (author)

  6. Situation analysis of quality of abortion care in the main maternity hospital in Hai Phòng, Viet Nam

    DEFF Research Database (Denmark)

    Nguyen, My H Ng; Gammeltoft, Tine; Rasch, Vibeke

    2007-01-01

    Six months after a Comprehensive Abortion Care project was implemented in Phu-San Hospital, the main maternity hospital in Hai Phòng, northern Viet Nam, a study of quality of abortion services was carried out. The study explored the interaction between providers and women seeking abortion and how...

  7. Hagkis ng Halakhak: Ang Nagpapatuloy na Kabuluhan ng Kritika ni Hernandez sa Pulitika sa Magkabilang Mukha ng Isang Bagol

    Directory of Open Access Journals (Sweden)

    Rosario Torres-Yu

    2012-12-01

    Full Text Available Itinanong ng kritikong si Epifanio San Juan, Jr., sa okasyon ng paggunita sa sentenaryo ng pagsilang ni Amado V. Hernandez, kung bakit wala pang panunuring historikal, liban sa isinulat ni Agoncillo at Lumbera, sa mga kuwento, dula, at sanaysay ng Pambansang Alagad sa Panitikan. Isang tugon ang panunuring ito sa isang dula ni Hernandez. Susuriin ang kahalagahang panlipunan at pangkasaysayan ng dula niya:1 sa lente mismo ng dalumat ni Hernandez sa relasyon ng panitikan at ng lipunan, 2 sa konteksto ng tagisang ideolohikal ng dula niya at ng panahong nasulat ang dula, at3 sa pangkasalukuyang sipat sa mga ito bilang diskurso na may diin sa usapin ng kapangyarihan at “katotohanan.” Malapitang susuriin, lalo na ang may kaugnayan sapangatlong pagbasa, ang dulang Magkabilang Mukha ng Isang Bagol (1961, dahil sa palagay ng nagsusuri na sa dulang ito higit na makikita na nagpapatuloy angkabuluhan ni Hernandez sa kasalukuyan, at dito lumulutang ang lakas at kapangyarihan ng kanyang kritika sa sistemang pampulitika.Writer and critic Epifanio San Juan Jr. observed, on the occasion of the centenary of the birth of Amado V. Hernandez, that except for the works of Agoncillo and Lumbera, no other historical analysis, particularly of the essays and plays, of the National Artist Hernandez has been written. This paper is an attempt to fill the gap. It evaluates the social and historical significance of one of his plays, Magkabilang Mukha ng Isang Bagol (1961, through the lens of Hernandez’s view of the relations of literature and society and in the context of the ideological position of his play and the ideological struggle atthe time the play was written. The play is treated, too, as discourse and centers on the discussion of power and “truth.” It is this writer’s position that it is in this play that Hernandez’s continuing relevance to the present ideological debaterests. It is also in this play that the strength of his critique is clearly

  8. Tracing Eurosceptic Party Networks via Hyperlink Network Analysis and #FAIL!ng: Can Web Crawlers Keep up with Web Design?

    DEFF Research Database (Denmark)

    Bossetta Jr, Michael Joseph; Dutceac Segesten, Anamaria

    This #FAIL! paper is the result of our experience with the hyperlink analysis software ‘Issuecrawler’ (www.govcom.org) whilst writing the paper “The Europeanization of Eurosceptics? A Hyperlink Network Analysis of the Sweden Democrats” for the 2015 European Consortium for Political Research (ECPR...

  9. H2NG (hydrogen-natural gas mixtures) effects on energy performances of a condensing micro-CHP (combined heat and power) for residential applications: An expeditious assessment of water condensation and experimental analysis

    International Nuclear Information System (INIS)

    In order to accomplish significant primary energy saving and GHG (greenhouse gas) emissions reduction, CHP (combined heat and power) technology can be adopted largely for industrial and civil sectors. Waiting for the cutting-edge appliances (i.e. Fuel Cell) wide deployment, ICEs (internal combustion engines) fuelled with an environmentally-friendly fuel, such as H2NG (hydrogen-natural gas mixtures) could represent the bridge technology towards the forthcoming pure hydrogen economy. This paper deals with the results of an experimental campaign carried out on a Single Cylinder ICE, fuelled with NG (natural gas) and H2NG @ 15% vol. In detail, energy performances were assessed at rated and partial loads. From data analysis, it emerged that the electrical efficiency increased up to 2.28%, at the expense of the heat recovery one, having added hydrogen. Additionally, due to the higher water content in exhaust gas when H2NG is burned, it was investigated on how heat recovery efficiency has been affected by condensing operating conditions. Finally, to estimate this benefit, an expeditious procedure was developed building three maps for H2NG blends condensing properties from 0% up to 30% vol. of H2. Their outputs provided the condensation efficiency value and the absolute gain of heat recovery one with varying exhaust gas temperatures and hydrogen fraction in the mixture. - Highlights: • H2NG mixture effects on a commercial technology μCHP for residential applications. • Experimental analysis and methodology for H2NG condensing properties assessment. • CO2 specific emissions calculation accounting for electrical and thermal outputs. • Uncertainty analysis on the μCHP energy performances and methodology validation. • H2 addition coupling a condensing heat exchanger enhance CHP First Law efficiency

  10. TestNG beginner's guide

    CERN Document Server

    Menon, Varun

    2013-01-01

    This book is written in a friendly, beginner's guide style with plenty of step-by-step instructions with appropriate examples.This book is great for developers and testers who are new to TestNg and want to learn how to use TestNG for writing their application as well as functional tests. This book assumes that you have experience in Java and OOPs concepts and have worked with certain IDE.

  11. Analysis of slow-onset neurite formation in NG108-15 cells: implications for a unified model of neurite elongation.

    Science.gov (United States)

    Smalheiser, N R

    1989-01-01

    When undifferentiated NG108-15 cells are plated onto polylysine coated Petri dishes in serum-free medium, they form neurites within 1-4 h if plated in the presence of laminin or 5'-deoxy-5'-methylthioadenosine (rapid-onset neurites). In the absence of such agents, serum-deprived NG108-15 cells extend axon-like neurites onto polylysine over several days; here we characterize the dynamic behavior of this slow-onset outgrowth pattern in detail. Individual cells plated on laminin expressed a gradual multipolar-to-unipolar transition due to rapid-onset neurites becoming remodelled into the appearance of slow-onset neurites. This phenomenon reflected the selective stabilization of certain rapid-onset neurites, along with the restriction of motility to their distal tips. Based upon the properties and interactions of both rapid- and slow-onset neurites in NG108-15 cells, a unified model for neurite formation is presented. PMID:2917412

  12. Serum 25-Hydroxyvitamin D Concentrations ≥40 ng/ml Are Associated with >65% Lower Cancer Risk: Pooled Analysis of Randomized Trial and Prospective Cohort Study.

    Directory of Open Access Journals (Sweden)

    Sharon L McDonnell

    Full Text Available Higher serum 25-hydroxyvitamin D [25(OHD] concentrations have been associated with a lower risk of multiple cancer types across a range of 25(OHD concentrations.To investigate whether the previously reported inverse association between 25(OHD and cancer risk could be replicated, and if a 25(OHD response region could be identified among women aged 55 years and older across a broad range of 25(OHD concentrations.Data from two cohorts representing different median 25(OHD concentrations were pooled to afford a broader range of 25(OHD concentrations than either cohort alone: the Lappe cohort (N = 1,169, a randomized clinical trial cohort (median 25(OHD = 30 ng/ml and the GrassrootsHealth cohort (N = 1,135, a prospective cohort (median 25(OHD = 48 ng/ml. Cancer incidence over a multi-year period (median: 3.9 years was compared according to 25(OHD concentration. Kaplan-Meier plots were developed and the association between 25(OHD and cancer risk was examined with multivariate Cox regression using multiple 25(OHD measurements and spline functions. The study included all invasive cancers excluding skin cancer.Age-adjusted cancer incidence across the combined cohort (N = 2,304 was 840 cases per 100,000 person-years (1,020 per 100,000 person-years in the Lappe cohort and 722 per 100,000 person-years in the GrassrootsHealth cohort. Incidence was lower at higher concentrations of 25(OHD. Women with 25(OHD concentrations ≥40 ng/ml had a 67% lower risk of cancer than women with concentrations <20 ng/ml (HR = 0.33, 95% CI = 0.12-0.90.25(OHD concentrations ≥40 ng/ml were associated with substantial reduction in risk of all invasive cancers combined.

  13. Serum 25-Hydroxyvitamin D Concentrations ≥40 ng/ml Are Associated with >65% Lower Cancer Risk: Pooled Analysis of Randomized Trial and Prospective Cohort Study

    Science.gov (United States)

    McDonnell, Sharon L.; Baggerly, Carole; French, Christine B.; Baggerly, Leo L.; Garland, Cedric F.; Gorham, Edward D.; Lappe, Joan M.; Heaney, Robert P.

    2016-01-01

    Background Higher serum 25-hydroxyvitamin D [25(OH)D] concentrations have been associated with a lower risk of multiple cancer types across a range of 25(OH)D concentrations. Objectives To investigate whether the previously reported inverse association between 25(OH)D and cancer risk could be replicated, and if a 25(OH)D response region could be identified among women aged 55 years and older across a broad range of 25(OH)D concentrations. Methods Data from two cohorts representing different median 25(OH)D concentrations were pooled to afford a broader range of 25(OH)D concentrations than either cohort alone: the Lappe cohort (N = 1,169), a randomized clinical trial cohort (median 25(OH)D = 30 ng/ml) and the GrassrootsHealth cohort (N = 1,135), a prospective cohort (median 25(OH)D = 48 ng/ml). Cancer incidence over a multi-year period (median: 3.9 years) was compared according to 25(OH)D concentration. Kaplan-Meier plots were developed and the association between 25(OH)D and cancer risk was examined with multivariate Cox regression using multiple 25(OH)D measurements and spline functions. The study included all invasive cancers excluding skin cancer. Results Age-adjusted cancer incidence across the combined cohort (N = 2,304) was 840 cases per 100,000 person-years (1,020 per 100,000 person-years in the Lappe cohort and 722 per 100,000 person-years in the GrassrootsHealth cohort). Incidence was lower at higher concentrations of 25(OH)D. Women with 25(OH)D concentrations ≥40 ng/ml had a 67% lower risk of cancer than women with concentrations <20 ng/ml (HR = 0.33, 95% CI = 0.12–0.90). Conclusions 25(OH)D concentrations ≥40 ng/ml were associated with substantial reduction in risk of all invasive cancers combined. PMID:27049526

  14. The Heat Flux Analysis in an Annulus Narrows Gap With Initial Temperature Variations Using HeaTiNG-01 Test Section

    International Nuclear Information System (INIS)

    An experiment to understand the complexity of boiling phenomena on a narrow gap, which has occurs in severe accident at TMI-2 NPP is necessary to be done in aimed to increase the understanding of accident management. The goal of research is to obtain a heat flux and critical heat flux (CHF) value during boiling heat transfer process in a narrow gap annulus. The method of research is experimental using HeaTiNG-01 test section. The experiment has been done with heating-up heated rod until a certain initial temperature, for this experiment, three initial temperature variations was decided at 650°C, 750°C dan 850°C. Then, a cooling process in heated rod by saturated water was recorded based on temperature data changes. Temperature data was used to calculate a value of heat flux and wall superheat temperature, until the results could be defined in boiling curve. The result of this research shows that, although the initial temperature of heated rod was different, the value of CHF is almost similar with CHF average 253.7 kW/m2 with the changes of only 4.7%. The event of boiling in a narrow gap is not included pool boiling category based on the comparison of film boiling area of the experiment to Bromley correlations. (author)

  15. Exploring the Nature of Silicon-Noble Gas Bonds in H3SiNgNSi and HSiNgNSi Compounds (Ng = Xe, Rn

    Directory of Open Access Journals (Sweden)

    Sudip Pan

    2015-03-01

    Full Text Available Ab initio and density functional theory-based computations are performed to investigate the structure and stability of H3SiNgNSi and HSiNgNSi compounds (Ng = Xe, Rn. They are thermochemically unstable with respect to the dissociation channel producing Ng and H3SiNSi or HSiNSi. However, they are kinetically stable with respect to this dissociation channel having activation free energy barriers of 19.3 and 23.3 kcal/mol for H3SiXeNSi and H3SiRnNSi, respectively, and 9.2 and 12.8 kcal/mol for HSiXeNSi and HSiRnNSi, respectively. The rest of the possible dissociation channels are endergonic in nature at room temperature for Rn analogues. However, one three-body dissociation channel for H3SiXeNSi and one two-body and one three-body dissociation channels for HSiXeNSi are slightly exergonic in nature at room temperature. They become endergonic at slightly lower temperature. The nature of bonding between Ng and Si/N is analyzed by natural bond order, electron density and energy decomposition analyses. Natural population analysis indicates that they could be best represented as (H3SiNg+(NSi− and (HSiNg+(NSi−. Energy decomposition analysis further reveals that the contribution from the orbital term (ΔEorb is dominant (ca. 67%–75% towards the total attraction energy associated with the Si-Ng bond, whereas the electrostatic term (ΔEelstat contributes the maximum (ca. 66%–68% for the same in the Ng–N bond, implying the covalent nature of the former bond and the ionic nature of the latter.

  16. Neuron-NG2 Cell Synapses: Novel Functions for Regulating NG2 Cell Proliferation and Differentiation

    Directory of Open Access Journals (Sweden)

    Qian-Kun Yang

    2013-01-01

    Full Text Available NG2 cells are a population of CNS cells that are distinct from neurons, mature oligodendrocytes, astrocytes, and microglia. These cells can be identified by their NG2 proteoglycan expression. NG2 cells have a highly branched morphology, with abundant processes radiating from the cell body, and express a complex set of voltage-gated channels, AMPA/kainate, and GABA receptors. Neurons notably form classical and nonclassical synapses with NG2 cells, which have varied characteristics and functions. Neuron-NG2 cell synapses could fine-tune NG2 cell activities, including the NG2 cell cycle, differentiation, migration, and myelination, and may be a novel potential therapeutic target for NG2 cell-related diseases, such as hypoxia-ischemia injury and periventricular leukomalacia. Furthermore, neuron-NG2 cell synapses may be correlated with the plasticity of CNS in adulthood with the synaptic contacts passing onto their progenies during proliferation, and synaptic contacts decrease rapidly upon NG2 cell differentiation. In this review, we highlight the characteristics of classical and nonclassical neuron-NG2 cell synapses, the potential functions, and the fate of synaptic contacts during proliferation and differentiation, with the emphasis on the regulation of the NG2 cell cycle by neuron-NG2 cell synapses and their potential underlying mechanisms.

  17. Ang proseso ng transkripsiyon ng mga inskripsiyon sa mga Bato ng Ticao

    Directory of Open Access Journals (Sweden)

    Ramon Guillermo

    2012-06-01

    Full Text Available The so-called ‘Ticao Stones’ that were announced to the public last 2011 were the first stone inscriptions in the baybayin script to have been discovered in the Philippines. This article is a report of the transcription of the inscription which was collectively produced by the interdisciplinary University of the Philippines (U.P. Ticao, Masbate Anthropological Project Team. This report includes a systematic analysis of the inscriptions and some suggestions towards a possible reading of the inscriptions. Some issues relevant to the problem of the authenticity of the stones also raised.

  18. SAS - en flygande beslutsgång

    OpenAIRE

    Haglund, Erik; Hjerpe, Anders

    2004-01-01

    A1146 Problem: De frågor som ställs är: Vilken slags beslutsgång och vilka beslutstyper det studerade företaget SAS har valt? Den klassiska rationella beslutsmodellen har fått utstå en viss kritik, men kan företag tillämpa modellen ändå? Vilka fördelar respektive nackdelar finns det om ett företag väljer att använda sig av en rationell beslutsgång? Syfte: Syftet med denna uppsats är att beskriva och analysera SAS koncernens beslutsgång. Det som avses med SAS:s beslutsgång är den förändringspr...

  19. Bitcoin-NG: A Scalable Blockchain Protocol

    OpenAIRE

    Eyal, Ittay; Gencer, Adem Efe; Sirer, Emin Gun; van Renesse, Robbert

    2015-01-01

    Cryptocurrencies, based on and led by Bitcoin, have shown promise as infrastructure for pseudonymous online payments, cheap remittance, trustless digital asset exchange, and smart contracts. However, Bitcoin-derived blockchain protocols have inherent scalability limits that trade-off between throughput and latency and withhold the realization of this potential. This paper presents Bitcoin-NG, a new blockchain protocol designed to scale. Based on Bitcoin's blockchain protocol, Bitcoin-NG is By...

  20. PVT-NG sensor final report.

    Energy Technology Data Exchange (ETDEWEB)

    Mitchell, Dean James; Brusseau, Charles A.

    2012-01-01

    This document is a final report for the polyvinyl toluene (PVT) neutron-gamma (PVT-NG) project, which was sponsored by the Domestic Nuclear Detection Office (DNDO). The PVT-NG sensor uses PVT detectors for both gamma and neutron detection. The sensor exhibits excellent spectral resolution and gain stabilization, which are features that are beneficial for detection of both gamma-ray and neutron sources. In fact, the ability to perform isotope identification based on spectra that were measured by the PVT-NG sensor was demonstrated. As described in a previous report, the neutron sensitivity of the first version of the prototype was about 25% less than the DNDO requirement of 2.5 cps/ng for bare Cf-252. This document describes design modifications that were expected to improve the neutron sensitivity by about 50% relative to the PVT-NG prototype. However, the project was terminated before execution of the design modifications after portal vendors demonstrated other technologies that enable neutron detection without the use of He-3. Nevertheless, the PVT-NG sensor development demonstrated several performance goals that may be useful in future portal designs.

  1. The NG2 Protein Is Not Required for Glutamatergic Neuron-NG2 Cell Synaptic Signaling.

    Science.gov (United States)

    Passlick, Stefan; Trotter, Jacqueline; Seifert, Gerald; Steinhäuser, Christian; Jabs, Ronald

    2016-01-01

    NG2 glial cells (as from now NG2 cells) are unique in receiving synaptic input from neurons. However, the components regulating formation and maintenance of these neuron-glia synapses remain elusive. The transmembrane protein NG2 has been considered a potential mediator of synapse formation and alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR) clustering, because it contains 2 extracellular Laminin G/Neurexin/Sex Hormone-Binding Globulin domains, which in neurons are crucial for formation of transsynaptic neuroligin-neurexin complexes. NG2 is connected via Glutamate Receptor-Interacting Protein with GluA2/3-containing AMPARs, thereby possibly mediating receptor clustering in glial postsynaptic density. To elucidate the role of NG2 in neuron-glia communication, we investigated glutamatergic synaptic transmission in juvenile and aged hippocampal NG2 cells of heterozygous and homozygous NG2 knockout mice. Neuron-NG2 cell synapses readily formed in the absence of NG2. Short-term plasticity, synaptic connectivity, postsynaptic AMPAR current kinetics, and density were not affected by NG2 deletion. During development, an NG2-independent acceleration of AMPAR current kinetics and decreased synaptic connectivity were observed. Our results indicate that the lack of NG2 does not interfere with genesis and basic properties of neuron-glia synapses. In addition, we demonstrate frequent expression of neuroligins 1-3 in juvenile and aged NG2 cells, suggesting a role of these molecules in synapse formation between NG2 glia and neurons. PMID:25100858

  2. Ang Kasaysayan at Panitikan sa Pagbuo ng Bayan: Rebyu ng Nobelang The Feet of Juan Bacnang ni F. Sionil Jose

    OpenAIRE

    Moreal Nagarit Camba

    2013-01-01

    Mababasa sa The Feet of Juan Bacnang (2011), ang pinakabagong nobela ni F. (Francisco) Sionil Jose o Frankie sa kanyang mga kaibigan, ang mahigpit na ugnayan ng manunulat at ng kanyang bayan. Siya ang nagsisilbing zeitgeist ng maraming panahon; ang espiritu at boses ng nakaraan at kasalukuyan, isang manunulat na kumakatha ng mga akdang sumasalamin sa mayamang karanasan at kasaysayan ng kultura at lahing pinag-usbungan. Kung saan ang kanyang nobelang Juan Bacnang, katulad ng iba pa niyang naun...

  3. Test Report for NG Sensors GTX-1000.

    Energy Technology Data Exchange (ETDEWEB)

    Manginell, Ronald P.

    2015-02-01

    This report describes initial testing of the NG Sensor GTX-1000 natural gas monitoring system. This testing showed that the retention time, peak area stability and heating value repeatability of the GTX-1000 were promising for natural gas measurements in the field or at the well head. The repeatability can be less than 0.25% for LHV and HHV for the Airgas standard tested in this report, which is very promising for a first generation prototype. Ultimately this system should be capable of 0.1% repeatability in heating value at significant size and power reductions compared with competing systems.

  4. Ang Kasaysayan at Panitikan sa Pagbuo ng Bayan: Rebyu ng Nobelang The Feet of Juan Bacnang ni F. Sionil Jose

    Directory of Open Access Journals (Sweden)

    Moreal Nagarit Camba

    2013-06-01

    Full Text Available Mababasa sa The Feet of Juan Bacnang (2011, ang pinakabagong nobela ni F. (Francisco Sionil Jose o Frankie sa kanyang mga kaibigan, ang mahigpit na ugnayan ng manunulat at ng kanyang bayan. Siya ang nagsisilbing zeitgeist ng maraming panahon; ang espiritu at boses ng nakaraan at kasalukuyan, isang manunulat na kumakatha ng mga akdang sumasalamin sa mayamang karanasan at kasaysayan ng kultura at lahing pinag-usbungan. Kung saan ang kanyang nobelang Juan Bacnang, katulad ng iba pa niyang naunang mga nobela, bagamat likhang-isip, ay repleksyon ng buhay, pasakit, at aspirasyon ng manunulat at lipunang lumikha rito; mga tunguhing hindi nalalayo sa layunin ng mga ninuno nitong anyong panitikan, ang mga epiko at kuwentong bayan. Ibig sabihin, ang akda bilang Weltanschauung na ayon kay Terry Eagleton ay “partikular na paraan ng pagtingin sa mundo; na may kaugnayan sa gahum na nagtatakda kung paano tingnan ang mundo na maituturing na ‘pag-iisip ng lipunan’ o ideyolohiya ng panahon”1 (sinipi ni Geok-Lin Lim A Modern National Epic 74. Dagdag pa rito, ang kanyang (mga nobela, partikular ang Juan Bacnang, ay nagsisilbing “siwang sa mga kurtinang-papel” sa kasaysayan;2 mga tala ng karanasan at pang-araw-araw na buhay, na madalas hindi naisusulat ng mga tradisyunal na historyador. Ito (ang mga tala, ayon kay Pierre Macherey, ang mga puwang o pinipi na boses na naisantabi ng maraming akdang pangkasaysayan (sinipi ni Eagleton Marxism and Literary Criticism 34-35.

  5. The Role of NG2 Proteoglycan in Glioma

    Directory of Open Access Journals (Sweden)

    Sridevi Yadavilli

    2016-02-01

    Full Text Available Neuron glia antigen-2 ((NG2, also known as chondroitin sulphate proteoglycan 4, or melanoma-associated chondroitin sulfate proteoglycan is a type-1 membrane protein expressed by many central nervous system (CNS cells during development and differentiation and plays a critical role in proliferation and angiogenesis. ‘NG2’ often references either the protein itself or the highly proliferative and undifferentiated glial cells expressing high levels of NG2 protein. NG2 glia represent the fourth major type of neuroglia in the mammalian nervous system and are classified as oligodendrocyte progenitor cells by virtue of their committed oligodendrocyte generation in developing and adult brain. Here, we discuss NG2 glial cells as well as NG2 protein and its expression and role with regards to CNS neoplasms as well as its potential as a therapeutic target for treating childhood CNS cancers.

  6. Post treatment PSA nadirs support continuing dose escalation study in patients with pretreatment PSA levels >10 ng/ml, but not in those with PSA <10 NG/ML

    International Nuclear Information System (INIS)

    Purpose: We have recently shown that ICRU reporting point radiation doses above 71 Gy are not associated with improved bNED survival in prostate cancer patients with pretreatment PSA level 20 ng/ml we found a strong correlation between dose and nadir values < 1.0 ng/ml (p=.003) as well as for nadir's < 0.5 ng/ml (p=.04). This dose/nadir effect held at several dose levels, but 74 Gy for nadir values < 1.0 ng/ml and 72 Gy for nadir's < 0.5 ng/ml remained the most significant. 32% of these patients achieved a nadir < 1.0ng/ml and 15% < 0.5ng/ml. Conclusions: This analysis provides strong additional support that patients with pretreatment PSA values of < 10 ng/ml do not benefit from dose escalation beyond an ICRU reporting point dose of 71 Gy. For patients with pretreatment PSA's of 10-19.9 ng/ml there is no dose/nadir response evaluated at a nadir of 1.0 ng/ml; however, there is a borderline effect observed at a nadir of 0.5 ng/ml. Patients with pretreatment PSA's of 20 ng/ml or greater clearly benefit from higher doses as evaluated by PSA nadirs of 1.0 ng/ml, and 0.5 ng/ml. These studies support the continued investigation of dose escalation in treating patients with PSA levels over 10 ng/ml, they do not support continued investigation of dose escalation beyond 71 Gy in patients with pretreatment PSA levels < 10 ng/ml. The failure to demonstrate any dose response for the low PSA group and the finding of only a borderline effect for the intermediate PSA group may be influenced by the relatively small number of patients in our series treated to doses < 70 Gy and the fact that none of our patients were treated to doses below 65.98 Gy. The lower limit of acceptible dose has yet to be defined

  7. Mga Kuwento-Kuwento ng Baliw: Lihis na Pagkilos sa Mga Maikling-Kuwentong Tagalog

    OpenAIRE

    Diana Ziganay; Ma. Trinidad Crisanto

    1990-01-01

    Ang sulating ito ay nalikha upang magbigay ng isang pagsusuri at pagtatalakay sa pagbuo ng konsepto ng abnormalidad o kakaibang kilos o pananaw buhat sa mga piling uri ng panitikang Pilipinong naisulat sa wikang Ingles noong ika-1900 hanggang 1945. Pag-uukulan ng pansin dito ang panahon at kalagayang binanggit, ang mga suliraning binanggit, ang maga suliranin ng pagbuo ng panitikang Pilipino sa Ingles, at ang daloy ng kaisipan ng mga manunulat na may kaugnayan sa pag-unawa ng konsepto ng abno...

  8. Mga Kuwento-Kuwento ng Baliw: Lihis na Pagkilos sa Mga Maikling-Kuwentong Tagalog

    Directory of Open Access Journals (Sweden)

    Diana Ziganay

    1990-12-01

    Full Text Available Ang sulating ito ay nalikha upang magbigay ng isang pagsusuri at pagtatalakay sa pagbuo ng konsepto ng abnormalidad o kakaibang kilos o pananaw buhat sa mga piling uri ng panitikang Pilipinong naisulat sa wikang Ingles noong ika-1900 hanggang 1945. Pag-uukulan ng pansin dito ang panahon at kalagayang binanggit, ang mga suliraning binanggit, ang maga suliranin ng pagbuo ng panitikang Pilipino sa Ingles, at ang daloy ng kaisipan ng mga manunulat na may kaugnayan sa pag-unawa ng konsepto ng abnormalidad na sumasalamin sa ilang mga piling sulatin, tuwiran man o di tuwiran.

  9. Etendatakse 10-aastase kirjamehe näitemäng

    Index Scriptorium Estoniae

    2003-01-01

    Tartu katoliku kooli 3. klassi poisi Mario Raitari näitemäng "Kristoph Silvester von Tenderi lugu", mis on esimene lugu sarjast "Mario Raitari kroonika". Näidendit etendab Linnupuu Lastepereteatri trupp

  10. NG6: Integrated next generation sequencing storage and processing environment

    Directory of Open Access Journals (Sweden)

    Mariette Jérôme

    2012-09-01

    Full Text Available Abstract Background Next generation sequencing platforms are now well implanted in sequencing centres and some laboratories. Upcoming smaller scale machines such as the 454 junior from Roche or the MiSeq from Illumina will increase the number of laboratories hosting a sequencer. In such a context, it is important to provide these teams with an easily manageable environment to store and process the produced reads. Results We describe a user-friendly information system able to manage large sets of sequencing data. It includes, on one hand, a workflow environment already containing pipelines adapted to different input formats (sff, fasta, fastq and qseq, different sequencers (Roche 454, Illumina HiSeq and various analyses (quality control, assembly, alignment, diversity studies,… and, on the other hand, a secured web site giving access to the results. The connected user will be able to download raw and processed data and browse through the analysis result statistics. The provided workflows can easily be modified or extended and new ones can be added. Ergatis is used as a workflow building, running and monitoring system. The analyses can be run locally or in a cluster environment using Sun Grid Engine. Conclusions NG6 is a complete information system designed to answer the needs of a sequencing platform. It provides a user-friendly interface to process, store and download high-throughput sequencing data.

  11. Tsapter 17 (Les Bambous de Paris ng Baybayin, l’Alphabet Syllabique des Tagals ni Jean-Paul G. Potet

    Directory of Open Access Journals (Sweden)

    Ramon Guillermo

    2013-12-01

    Full Text Available A comprehensive book on the baybayin writing system was recently published by the French linguist Jean-Paul Potet. One of the most interesting chapters is a transcription and analysis of baybayin inscriptions written on bamboos which are currently in the safekeeping of the Musée du Quai Branly, Paris with inventory numbers 71-1932-11-13 to -20. What follows is a review of Potet’s book and a translation of the chapter on the bamboos of Paris. Lumabas kamakailan lamang ang isang kumprehensibong aklat hinggil sa sistema ng pagsusulat na baybayin ng Pranses na linggwista na si Jean-Paul Potet. Ang isa sa mga pinakainteresanteng tsapter nito ay naglalaman ng isang transkripsiyon at pagsusuri ng mga inskripsiyong baybayin na nakaukit sa mga kawayan na kasalukuyang nasa pangangalaga ng Musée du Quai Branly, Paris kung saan ang bago nilang mga numerong imbentaryo ay 71- 1932-11-13 hanggang -20. Ang sumusunod ay isang rebyu ng aklat ni Potet at pagsasalin ng tsapter hinggil sa mga kawayan ng Paris.

  12. City-gas development in China-An NG perspective

    International Nuclear Information System (INIS)

    Supply of piped gas in China has a long history but development was slow until the 21st century. The growing demand for cleaner fuel in the past ten years has encouraged domestic exploration and production of natural gas (NG) and the construction of long-haul pipelines linking upstream western regions of the country to downstream city-gas consumers in the eastern coastal areas. However, demand for NG in the cities is increasing so fast that recent winters have seen severe supply difficulties. This led to a government directive which set constraints on the domestic NG supplies and discouragement to gas power and petrochemical projects, and eventually to the Chinese government's willingness to accept the higher prices commanded by the international NG market. Nevertheless, the over demand situation has created a dominant market position for upstream NG producers and long-haul pipeline operators favouring their forward integration into downstream city-gas markets. Accordingly, pressures are building that may yet result in substantial pricing and regulatory reform in the city-gas industry.

  13. City-gas development in China. An NG perspective

    International Nuclear Information System (INIS)

    Supply of piped gas in China has a long history but development was slow until the 21st century. The growing demand for cleaner fuel in the past ten years has encouraged domestic exploration and production of natural gas (NG) and the construction of long-haul pipelines linking upstream western regions of the country to downstream city-gas consumers in the eastern coastal areas. However, demand for NG in the cities is increasing so fast that recent winters have seen severe supply difficulties. This led to a government directive which set constraints on the domestic NG supplies and discouragement to gas power and petrochemical projects, and eventually to the Chinese government's willingness to accept the higher prices commanded by the international NG market. Nevertheless, the over demand situation has created a dominant market position for upstream NG producers and long-haul pipeline operators favouring their forward integration into downstream city-gas markets. Accordingly, pressures are building that may yet result in substantial pricing and regulatory reform in the city-gas industry. (author)

  14. Ang Demokratikong Sistema at ang mga Modelo ng Pamumuno sa Pilipinas

    Directory of Open Access Journals (Sweden)

    F. P. A. Demeterio

    2010-06-01

    Full Text Available Maraming paraan ang pagsusuri sa problema ng pulitika sa Pilipinas.Para sa mga moralista at ideyalista, halimbawa, maaari itong tingnangamit ang perspektibo ng sistema ng pagpapahalaga (values at moralidad; para sa mga materyalista naman, maaari din itong himayin gamit ang perspektibo ng pagka-di-pantay-pantay ng kayamanan at kapangyarihan(unequal wealth and power ng ating mga mamamayan; o hindi kaya para sa mga eksperto sa sistema at istraktura, maaari din itong dalumatin gamit ang perspektibo ng ating marupok na burukrasya. Susubukan ng papel na ito na pag-aralan ang paksa sa pamamagitan ng pagsusuri sa konsepto ng pamumuno na hinihiling ng ating demokratikong sistema at sa kaparehong konseptong umiiiral naman sa kamalayan ng ating mga kababayan. Gagamitin ng pagsusuring ito, bilang teoretikal na balangkas, ang isang uri ng antropolohikalna teorya at pamamaraan na kilala bilang “kognitibong antropolohiya”(cognitive anthropology.

  15. Noble-Gas-Inserted Fluoro(sulphido)boron (FNgBS, Ng = Ar, Kr, and Xe): A Theoretical Prediction.

    Science.gov (United States)

    Ghosh, Ayan; Dey, Sourav; Manna, Debashree; Ghanty, Tapan K

    2015-06-01

    The possibility of the existence of a new series of neutral noble gas compound, FNgBS (where Ng = Ar, Kr, Xe), is explored theoretically through the insertion of a Ng atom into the fluoroborosulfide molecule (FBS). Second-order Møller-Plesset perturbation theory, density functional theory, and coupled cluster theory based methods have been employed to predict the structure, stability, harmonic vibrational frequencies, and charge distribution of FNgBS molecules. Through energetics study, it has been found that the molecules could dissociate into global minima products (Ng + FBS) on the respective singlet potential energy surface via a unimolecular dissociation channel; however, the sufficiently large activation energy barriers provide enough kinetic stability to the predicted molecules, which, in turn, prevent them from dissociating into the global minima products. Moreover, the FNgBS species are thermodynamically stable, owing to very high positive energies with respect to other two two-body dissociation channels, leading to FNg + BS and F(-) + NgBS(+), and two three-body dissociation channels, corresponding to the dissociation into F + Ng + BS and F(-) + Ng + BS(+). Furthermore, the Mulliken and NBO charge analysis together with the AIM results reveal that the Ng-B bond is more of covalent in nature, whereas the F-Ng bond is predominantly ionic in character. Thus, these compounds can be better represented as F(-)[NgBS](+). This fact is also supported by the detail analysis of bond length, bond dissociation energy, and stretching force constant values. All of the calculated results reported in this work clearly indicate that it might be possible to prepare and characterize the FNgBS molecules in cryogenic environment through matrix isolation technique by using a mixture of OCS/BF3 in the presence of large quantity of noble gas under suitable experimental conditions. PMID:25928588

  16. Ablation of NG2 proteoglycan leads to deficits in brown fat function and to adult onset obesity.

    Directory of Open Access Journals (Sweden)

    Yunchao Chang

    Full Text Available Obesity is a major health problem worldwide. We are studying the causes and effects of obesity in C57Bl/6 mice following genetic ablation of NG2, a chondroitin sulfate proteoglycan widely expressed in progenitor cells and also in adipocytes. Although global NG2 ablation delays early postnatal adipogenesis in mouse skin, adult NG2 null mice are paradoxically heavier than wild-type mice, exhibiting larger white fat deposits. This adult onset obesity is not due to NG2-dependent effects on CNS function, since specific ablation of NG2 in oligodendrocyte progenitors yields the opposite phenotype; i.e. abnormally lean mice. Metabolic analysis reveals that, while activity and food intake are unchanged in global NG2 null mice, O(2 consumption and CO(2 production are decreased, suggesting a decrease in energy expenditure. Since brown fat plays important roles in regulating energy expenditure, we have investigated brown fat function via cold challenge and high fat diet feeding, both of which induce the adaptive thermogenesis that normally occurs in brown fat. In both tests, body temperatures in NG2 null mice are reduced compared to wild-type mice, indicating a deficit in brown fat function in the absence of NG2. In addition, adipogenesis in NG2 null brown pre-adipocytes is dramatically impaired compared to wild-type counterparts. Moreover, mRNA levels for PR domain containing 16 (PRDM16 and peroxisome proliferator-activated receptor γ coactivator (PGC1-α, proteins important for brown adipocyte differentiation, are decreased in NG2 null brown fat deposits in vivo and NG2 null brown pre-adipocytes in vitro. Altogether, these results indicate that brown fat dysfunction in NG2 null mice results from deficits in the recruitment and/or development of brown pre-adipocytes. As a consequence, obesity in NG2 null mice may occur due to disruptions in brown fat-dependent energy homeostasis, with resulting effects on lipid storage in white adipocytes.

  17. Theranostic impact of NG2/CSPG4 proteoglycan in cancer.

    Science.gov (United States)

    Nicolosi, Pier Andrea; Dallatomasina, Alice; Perris, Roberto

    2015-01-01

    NG2/CSPG4 is an unusual cell-membrane integral proteoglycan widely recognized to be a prognostic factor, a valuable tool for ex vivo and non-invasive molecular diagnostics and, by virtue of its tight association with malignancy, a tantalizing therapeutic target in several tumour types. Although the biology behind its involvement in cancer progression needs to be better understood, implementation of NG2/CSPG4 in the routine clinical practice is attainable and has the potential to contribute to an improved individualized management of cancer patients. In this context, its polymorphic nature seems to be particularly valuable in the effort to standardize informative diagnostic procedures and consolidate forcible immunotherapeutic treatment strategies. We discuss here the underpinnings for this potential and highlight the benefits of taking advantage of the intra-tumour and inter-patient variability in the regulation of NG2/CSPG4 expression. We envision that NG2/CSPG4 may effectively be exploited in therapeutic interventions aimed at averting resistance to target therapy agents and at interfering with secondary lesion formation and/or tumour recurrence. PMID:25767619

  18. Quasicrystals -- The impact of N.G. de Bruijn

    CERN Document Server

    Au-Yang, Helen

    2013-01-01

    In this paper we put the work of Professor N.G. de Bruijn on quasicrystals in historical context. After briefly discussing what went before, we shall review de Bruijn's work together with recent related theoretical and experimental developments. We conclude with a discussion of Yang-Baxter integrable models on Penrose tilings, for which essential use of de Bruijn's work has been made.

  19. Klaaspärlimäng peletab klassikahirmu / Katrin Nielsen

    Index Scriptorium Estoniae

    Nielsen, Katrin

    2003-01-01

    Eri stiile-ajastuid ühendavast Peeter Vähi kujandatud muusikafestivalist "Klaaspärlimäng" 22. juulist-24. augustini. Kontsertidest Pärnu kontserdimajas: gamelaniansambli Gamelan Son of Lion kontserdist 22. juulil, Vardo Rumesseni kontserdist ja CD esitlusest 28. juulil, tütarlastekoori "Ellerhein" kontserdist 30. juulil

  20. Mga Lente sa Likod ng Lente: Isang Panimulang Pag-aaral ng Ilang Litratong Kuha ni Xander Angeles

    Directory of Open Access Journals (Sweden)

    Moreal Nagarit Camba

    2011-12-01

    Full Text Available Ang pangalang Xander Angeles ay kilala bilang isa sa mga in-demand napangalan sa mundo ng fashion at advertisement sa Pilipinas. Kakabit ngpangalang ito ang samut-saring lokal at internasyunal na parangal; idagdag pa rito ang isang advertising company, isang modelling agency, at isang fashion and photography school.

  1. Ang Demokratikong Sistema at ang mga Modelo ng Pamumuno sa Pilipinas

    OpenAIRE

    F. P. A. Demeterio

    2010-01-01

    Maraming paraan ang pagsusuri sa problema ng pulitika sa Pilipinas.Para sa mga moralista at ideyalista, halimbawa, maaari itong tingnangamit ang perspektibo ng sistema ng pagpapahalaga (values) at moralidad; para sa mga materyalista naman, maaari din itong himayin gamit ang perspektibo ng pagka-di-pantay-pantay ng kayamanan at kapangyarihan(unequal wealth and power) ng ating mga mamamayan; o hindi kaya para sa mga eksperto sa sistema at istraktura, maaari din itong dalumatin gamit ang perspek...

  2. Meta Analisis sa Pagsusuri ng Maiikling Kwento sa mga Tesis at Disertasyon

    OpenAIRE

    Regina I. Cuizon

    2014-01-01

    Anumang mga pagbabago na makikita sa mundo ay bunga ng pananaliksik. Ang meta-analisis ay isa sa mga bunga ng pananaliksik na ginagamit sa kasalukuyan bilang teknik upang malaman iba pang mga nagsulputang informasyon. Ito’y pag-aaral sa mga pag-aaral. Isang kritikal at sistematikong pagsusuri sa istruktura ng mga pag-aaral. Maging gabay sa mga gradwadong paaralan sa pagpili ng paksang pagaaralan. Pangunahing layunin na matiyak ang mga pamamaraan sa pagsusuri ng maiikling kwento ng...

  3. Physically Based Rendering in the Nightshade NG Visualization Platform

    Science.gov (United States)

    Berglund, Karrie; Larey-Williams, Trystan; Spearman, Rob; Bogard, Arthur

    2015-01-01

    This poster describes our work on creating a physically based rendering model in Nightshade NG planetarium simulation and visualization software (project website: NightshadeSoftware.org). We discuss techniques used for rendering realistic scenes in the universe and dealing with astronomical distances in real time on consumer hardware. We also discuss some of the challenges of rewriting the software from scratch, a project which began in 2011.Nightshade NG can be a powerful tool for sharing data and visualizations. The desktop version of the software is free for anyone to download, use, and modify; it runs on Windows and Linux (and eventually Mac). If you are looking to disseminate your data or models, please stop by to discuss how we can work together.Nightshade software is used in literally hundreds of digital planetarium systems worldwide. Countless teachers and astronomy education groups run the software on flat screens. This wide use makes Nightshade an effective tool for dissemination to educators and the public.Nightshade NG is an especially powerful visualization tool when projected on a dome. We invite everyone to enter our inflatable dome in the exhibit hall to see this software in a 3D environment.

  4. Nogo receptor 3, a paralog of Nogo-66 receptor 1 (NgR1),may function as a NgR1 co-receptor for Nogo-66

    Institute of Scientific and Technical Information of China (English)

    Lei Zhang; Xia Kuang; Jian Zhang

    2011-01-01

    Nogo-A is a major myelin associated inhibitor that blocks regeneration of injured axons in the central nervous system (CNS).Nogo-66 (a 66-residue domain of Nogo-A) expressed on the surface of oligodendrocytes has been shown to directly interact with Nogo-66 receptor 1 (NgR1).A number of additional components of NgR1 receptor complex essential for its signaling have been uncovered.However,detailed composition of the complex and its signaling mechanisms remain to be fully elucidated.In this study,we show that Nogo receptor 3 (NgR3),a paralog of NgR1,is a binding protein for NgR1.The interaction is highly specific because other members of the reticulin family,to which Nogo-A belongs,do not bind to NgR3.Neither does NgR3 show any binding activity with Nogo receptor 2 (NgR2),another NgR1 paralog.Majority of NgR3 domains are required for its binding to NgR1.Moreover,a truncated NgR3 with the membrane anchoring domain deleted can function as a decoy receptor to reverse neurite outgrowth inhibition caused by Nogo-66 in culture.These in vitro results,together with previously reported overlapping expression profile between NgR1 and NgR3,suggest that NgR3 may be associated with NgR1 in vivo and that their binding interface may be targeted for treating neuronal injuries.

  5. BUbiNG: Massive Crawling for the Masses

    OpenAIRE

    Boldi, Paolo; Marino, Andrea; Santini, Massimo; Vigna, Sebastiano

    2016-01-01

    Although web crawlers have been around for twenty years by now, there is virtually no freely available, opensource crawling software that guarantees high throughput, overcomes the limits of single-machine systems and at the same time scales linearly with the amount of resources available. This paper aims at filling this gap, through the description of BUbiNG, our next-generation web crawler built upon the authors' experience with UbiCrawler [Boldi et al. 2004] and on the last ten years of res...

  6. La frase nominal Ngäbére

    Directory of Open Access Journals (Sweden)

    Murillo Miranda José Manuel

    2009-12-01

    Full Text Available Este trabajo es el avance de una amplia investigación cuyo propósito es elaborar una gramática descriptiva de la lengua chibcha ngäbére. El artículo presenta las características particulares más relevantes de la frase nominal de dicha variante dialectal (hablada en Costa Rica, también en Panamá, y establece la relación con algunas de las características generales de otras lenguas chibchas ístmicas. El primer apartado del escrito es una introducción a la lengua ngäbére y a sus hablantes; elsegundo presenta la morfología de los sustantivos, pronombres y otras clases de palabras que conforman la frase nominal; el tercer apartado trata de la sintaxis de la frase nominal y esboza, de manera sucinta, las relaciones gramaticales; el cuarto apartado es una conclusión valorativa de este trabajo.

  7. Use of Roud Wood in Kaingáng Houses

    Directory of Open Access Journals (Sweden)

    Ricardo Dias Silva

    2002-01-01

    Full Text Available This work has the purpose of describing and analyzing the evolutionary habitation process of the Kaingáng. Its main focus is the typological reading of the shelters as well as the registration of the wooden building system used in the construction of the houses in the “Äpucaraninha” Reserve, located in a district of Londrina. The research proceeds with the elaboration of the genealogy of the kaingáng’s habitation through the consultation to the published historical reports and their chronological organization. The data collection within the group was accomplished through informal interviews, photographic survey and in loco observations. Aspects related to the use of the round wood as well as the other elements that are part of the construction, were verified at this stage. The raw material used in every building system is obtained in the Reserve itself, being the wood used not only in the structure but also in the sealing. The connections are made through fittings and fastenings with liana. In spite of the mixture of values and meanings that the Kaingáng community experiences through the contact with other cultures, traditional aspects related to the building of the “traditional house” are preserved, with no domain of other techniques by most of the group. The registration intends to contribute towards the identification and understanding of important issues related to the spaces built by the man.

  8. The role of NgR-Rhoa-Rock signal pathway in retinal ganglion cell apoptosis of early diabetic rats

    Directory of Open Access Journals (Sweden)

    Yun-Jie Fu

    2014-09-01

    Full Text Available AIM: To study the function and mechanism of the NgR-Rhoa-Rock signal pathways which exists in the retinal ganglion cells apoptosis in diabetes mellitus(DMrats. METHODS: Some healthy SD rats were operated by means of single intraperitoneal injection of 1% streptozotocin based on the standard of 50mg/kg wight, after that the blood sugar value was greater than 16.7mmol/L as DM model, then randomly divided into 3 groups, each group was 10 rats. In addition to take 10 healthy SD rats as control group. Four groups of rats were bilaterally eyeball intravitreal injection in turn with NgR-siRNA virus 10μL(siRNA group, NgR-siRNA virus diluted 10μL(DM group, NgR-siRNA virus-negative-control solution 10μL(siRNA blank group, NgR-siRNA virus diluted 10μL(normal control group, and fed normally. During that time, some life indexes like blood glucose, body mass, etc. were measured and recorded. After 12wk, the expression of NgR and Rhoa, HE staining, and TUNNEL staining were detected by Western blot analysis. RESULTS: Western blot analysis: compared with normal control group, the expression of NgR and Rhoa in DM group and siRNA blank group increased significantly(PP>0.05; compared with DM group and siRNA blank group, the expression of those proteins significantly lowered in siRNA group. HE staining: compared with normal control group, some extent ganglion cells arranged disorder, irregular shape, spacing not consistent were all found in three groups of model rats; compared with DM group and siRNA blank group, there was some improvement in siRNA group of ganglion cells about the order and shape size. TUNEL staining: compared with normal control group, there were retinal ganglion cells apoptosis in all of three groups of model rats. Compared with DM group and siRNA blank group, the number of retinal ganglion cells apoptotic cells was less, and the shape of cells had improved significantly in siRNA group. CONCLUSION: In the DM phase, the expression of NgR and

  9. Here we are all brothers: gender relations and the construction of masculine identities in a Nùng Fan Slìng village

    OpenAIRE

    Wangsgard, David Brooke

    2009-01-01

    Recent scholarship surrounding questions of masculinity demonstrates that masculinity is not a natural, homogeneous category but a social construction that varies across space and time. This ethnography explores what constitutes masculinity among the Nùng Fan Slìng (Nùng), a Tai-speaking ethnic minority living in Northeastern Vietnam, through an examination of cultural assumptions that premise social practices and relationships that construct and reproduce gendered identities. Data, generated...

  10. Salman Rushdie: "Troonide mäng" on rämps

    Index Scriptorium Estoniae

    2011-01-01

    Oma esimest telesarja "Järgmised inimesed" kirjutav Salman Rushdie ei hinda USA telekanali HBO seriaali "Troonide mäng" (George R. R. Martini "Jää ja tule laulu" sarja esimese raamatu "Troonide mäng" teleekraniseering)

  11. Isang Reaksyon sa “Ang Demokratikong Sistema at ang mga Modelo ng Pamumuno sa Pilipinas”

    Directory of Open Access Journals (Sweden)

    Paolo A. Bolanos

    2010-06-01

    Full Text Available Kinukundisyon ng nalalapit na eleksyon sa Mayo ang ating kolektibongpag-iisip bilang mamamayan ng Pilipinas. Bahagyang hinuhubog nitoang ating kilos at salita na syang kumakatawan sa ating pag-asa at mgapangarap para sa ating sarili at para sa ating bansa. Dahil nakatuon ang atensyon natin sa nalalapit na eleksyon at dahil hindi maiwasang pag-usapan ang mga pambansang isyu na kadalasa’y mga pambansang suliranin (tulad ng kurapsyon at kahirapan, nagkakaroon tayo ng natatangi at mas malawak na pag-mumuni-muni sa ating sosyoholikal at politikal na sitwasyon, na tila namang nambubuyo sa ating pakiramdam ng kabiguan. Talaga naman na ang panahon ng eleksyon ay parehong panahon ng pag-asa at kabiguan—kadalasa’y ang pangalawa ang nananatiling realidad ng mga Pilipino!

  12. Isang Reaksyon sa “Ang Demokratikong Sistema at ang mga Modelo ng Pamumuno sa Pilipinas”

    OpenAIRE

    Paolo A. Bolanos

    2010-01-01

    Kinukundisyon ng nalalapit na eleksyon sa Mayo ang ating kolektibongpag-iisip bilang mamamayan ng Pilipinas. Bahagyang hinuhubog nitoang ating kilos at salita na syang kumakatawan sa ating pag-asa at mgapangarap para sa ating sarili at para sa ating bansa. Dahil nakatuon ang atensyon natin sa nalalapit na eleksyon at dahil hindi maiwasang pag-usapan ang mga pambansang isyu na kadalasa’y mga pambansang suliranin (tulad ng kurapsyon at kahirapan), nagkakaroon tayo ng natatangi at mas malawak na...

  13. New generation of naval IRST: example of EOMS NG

    Science.gov (United States)

    Maltese, Dominique; Deyla, Olivier; Vernet, Guillaume; Preux, Carole; Hilt, Gisèle; Nougues, Pierre-Olivier, II

    2010-04-01

    Modern warships ranging from Air Warfare Destroyers to Offshore Patrol Vessels (OPV) and Fast Patrol Boats have to deal with an ever increasing variety of threats, both symmetric and asymmetric, for self-protection. This last category has introduced new requirements for combat systems sensors and effectors: situation awareness in proximity of the own ship has become a priority, as well as the need for new, lethal or non-lethal effectors for timely and proportional response. Naval Combat Systems (CS) architects are then faced with an alternative: they can either use existing CS sensors, C2 and weapons, or else rely on new, specialized equipments. Both approaches have their pros and cons, with the cost issue not necessarily trivial to assess. In this paper, we present a multifunction system that is both a passive IRST (InfraRed Search and Track) sensor, designed to automatically detect and track air and surface threats, and an Electro Optical Director (EOD), capable of providing identification of objects as well as accurate 3D tracks. Following an introduction reviewing the design goals for the equipment, the EOMS NG processing architecture is described (Image & Tracking Processes). Then, system performances are presented for different scenarios provided from Field Tests.

  14. Recce NG: from Recce sensor to image intelligence (IMINT)

    Science.gov (United States)

    Larroque, Serge

    2001-12-01

    Recce NG (Reconnaissance New Generation) is presented as a complete and optimized Tactical Reconnaissance System. Based on a new generation Pod integrating high resolution Dual Band sensors, the system has been designed with the operational lessons learnt from the last Peace Keeping Operations in Bosnia and Kosovo. The technical solutions retained as component modules of a full IMINT acquisition system, take benefit of the state of art in the following key technologies: Advanced Mission Planning System for long range stand-off Manned Recce, Aircraft and/or Pod tasking, operating sophisticated back-up software tools, high resolution 3D geo data and improved/combat proven MMI to reduce planning delays, Mature Dual Band sensors technology to achieve the Day and Night Recce Mission, including advanced automatic operational functions, as azimuth and roll tracking capabilities, low risk in Pod integration and in carrier avionics, controls and displays upgrades, to save time in operational turn over and maintenance, High rate Imagery Down Link, for Real Time or Near Real Time transmission, fully compatible with STANAG 7085 requirements, Advanced IMINT Exploitation Ground Segment, combat proven, NATO interoperable (STANAG 7023), integrating high value software tools for accurate location, improved radiometric image processing and open link to the C4ISR systems. The choice of an industrial Prime contractor mastering across the full system, all the prior listed key products and technologies, is mandatory to a successful delivery in terms of low Cost, Risk and Time Schedule.

  15. Meta Analisis sa Pagsusuri ng Maiikling Kwento sa mga Tesis at Disertasyon

    Directory of Open Access Journals (Sweden)

    Regina I. Cuizon

    2014-10-01

    Full Text Available Anumang mga pagbabago na makikita sa mundo ay bunga ng pananaliksik. Ang meta-analisis ay isa sa mga bunga ng pananaliksik na ginagamit sa kasalukuyan bilang teknik upang malaman iba pang mga nagsulputang informasyon. Ito’y pag-aaral sa mga pag-aaral. Isang kritikal at sistematikong pagsusuri sa istruktura ng mga pag-aaral. Maging gabay sa mga gradwadong paaralan sa pagpili ng paksang pagaaralan. Pangunahing layunin na matiyak ang mga pamamaraan sa pagsusuri ng maiikling kwento ng mga tesis at disertasyong nagawa mula sa mga piling SUCs. Desinyong kwalitatibo - kontent analisis sa pagsusuri ng: kaanyuan ayon sa suliranin, metodolohiya, paglalahad at interpretasyon ng mga datos, natuklasan, konklusyon, at rekomendasyon; kahinatnan ayon sa pagkatulad, pagkakaiba at kabuluhan; Emerging tema. Napag-alaman na ang karaniwang pinag-aralan ay 30% kahalagahang pangkatauhan, 20% larawangdiwa ng mga kababaihan at 10% gramatikang aspeto. Sa metodolohiyang ginamit, 80% desinyong kwalitatibo at 20% kwantitatibo-kwalitatibo. Sa paglikom ng datos 60% diretsahang pagsusuri, 30% talatanungan at 10% tseklis. Sa pag-analisa 90% kontent analisis at 10% gramatikal analisis. Batay sa natuklasan, ang pagsusuri ng maiikling kwento sa mga tesis at disertasyon mula sa iba’t ibang paaralan gamit ang meta analisis ay isang epektibo, mabisa, objektibong paraan at kagamitan na magagamit sa makatarungang paghatol; pamumuna sa kabuluhan at kagandahan; paghaham- bing sa mga kritikal na isyu; at pormulasyon ng panibagong pamantayan at batas.

  16. 76 FR 12627 - Airworthiness Directives; Diamond Aircraft Industries GmbH Models DA 42, DA 42 NG, and DA 42 M-NG...

    Science.gov (United States)

    2011-03-08

    ... the MCAI, on November 23, 2010, we issued AD 2010-25-01, Amendment 39-16534 (75 FR 75868, December 7... Order 12866; 2. Is not a ``significant rule'' under the DOT Regulatory Policies and Procedures (44 FR... Industries GmbH Models DA 42, DA 42 NG, and DA 42 M-NG Airplanes AGENCY: Federal Aviation Administration...

  17. NG2细胞与中枢神经系统疾病%Roles of NG2 glial cells in diseases of the central nervous system

    Institute of Scientific and Technical Information of China (English)

    许建平; 赵杰; 李韶

    2011-01-01

    NG2 cells are a novel distinct class of central nervous system(CNS)glial cells,characterized by the expression of the chondroitin sulfate proteoglycan NG2.They have been detected in a variety of human CNS diseases.As morphological,physiological and biomolecular studies of NG2 cells have been conducted,their roles have been gradually revealed.Research on cellular and molecular mechanisms in the pathophysiological state was built on the preliminary findings of their physiological functions; and in turn,this helps to clarify their physiological roles and leads to the identification of novel therapeutic targets.This review summarizes recent findings regarding the potential roles of NG2 cells in traumatic brain injury,multiple sclerosis,glioma,epilepsy,Alzheimer's disease and electroconvulsive therapy for depression.%NG2细胞是新发现的一类广泛存在于成熟和发育期中枢神经系统的胶质细胞群体.这些细胞表面表达NG2硫酸软骨素蛋白多糖,因而常被称作NG2细胞.随着NG2细胞形态学研究的深入,NG2胶质细胞的功能也越来越受到关注.NG2细胞在人类多种中枢神经系统疾病中扮演重要角色.本文结合最新的研究报道,就其在一些常见的中枢神经系统疾病中的作用进行概括综述.

  18. SomethiNG 2 talk about-Transcriptional regulation in embryonic and adult oligodendrocyte precursors.

    Science.gov (United States)

    Küspert, Melanie; Wegner, Michael

    2016-05-01

    Glial cells that express the chondroitin sulfate proteoglycan NG2 represent an inherently heterogeneous population. These so-called NG2-glia are present during development and in the adult CNS, where they are referred to as embryonic oligodendrocyte precursors and adult NG2-glia, respectively. They give rise to myelinating oligodendrocytes at all times of life. Over the years much has been learnt about the transcriptional network in embryonic oligodendrocyte precursors, and several transcription factors from the HLH, HMG-domain, zinc finger and homeodomain protein families have been identified as main constituents. Much less is known about the corresponding network in adult NG2-glia. Here we summarize and discuss current knowledge on functions of each of these transcription factor families in NG2-glia, and where possible compare transcriptional regulation in embryonic oligodendrocyte precursors and adult NG2-glia. This article is part of a Special Issue entitled SI:NG2-glia (Invited only). PMID:26232072

  19. Study on Interaction of ADN and (NC+NG) by Gasometric Method%量气法研究ADN与(NC+NG)的相互作用

    Institute of Scientific and Technical Information of China (English)

    何少蓉; 张林军; 衡淑云; 刘子如

    2008-01-01

    用 NBK型"拉瓦"量气测试系统研究了较高温度下二硝酰胺铵(ADN)与双基吸收药(NC+NG)体系的相互作用,从动力学角度阐述了二者的相互作用.结果表明: ADN与(NC+NG)混合物加热分解的最终放气量在标准状态下为626.8 mL·g-1,与ADN和(NC+NG)最大放气量之和相比并没有增加,但分解速度显著提高,活化能显著降低至82.58 kJ·mol-1,ADN与(NC+NG)之间存在强烈的相互作用.

  20. Basin-scale characterization of river hydromorphology by map derived information: A case study on the Red River (Sôngng), Vietnam

    Science.gov (United States)

    Schmitt, R. J.; Bizzi, S.; Castelletti, A.

    2012-12-01

    The understanding of river hydromorphological processes has been recognized in the last decades as a priority of modern catchment management, since fluvial geomorphic processes shape physical habitat, affect river infrastructures and influence freshwater ecological processes. Characterization of river hydromorphological features is commonly location specific and highly demanding in terms of field-works, resource and expertise required. Therefore, its routine application at regional or national scales, although an urgent need of catchment management, is infeasible at present. Recently available high-resolution data, such as DEM or LIDAR, opens up novel potential for basin-wide analysis of fluvial processes at limited effort and cost. Specifically, in this study we assess the feasibility of characterizing river hydromorphology from specific map derived geomorphic controls namely: channel gradient, bankfull flow, specific stream power, and degree of channel confinement. The river network, extracted from a digital elevation model and validated with available network shape-files and optical satellite imagery, available flow gauging stations and GIS processing allow producing continuous values of geomorphic drivers defined over given length segments at catchment or regional scales. This generic framework was applied to the Red River (Sôngng) basin, the second largest basin (87,800 km2) in Vietnam. Besides its economic importance, the river since few years is experiencing severe river bed incisions due to the building of new dams in the upstream part of the catchment and sand mining in the surrounding of the capital city Hanoi. In this context, characterized by an high developing rate, current efforts to increase water productivity by infrastructure and management measures require a thorough understanding of fluvial system and, in particular, of the basin-wide river hydromorphology. The framework proposed has allowed producing high-dimensional samples of spatially

  1. NG2 expression in glioblastoma identifies an actively proliferating population with an aggressive molecular signature

    OpenAIRE

    Al-Mayhani, M. Talal F.; Grenfell, Richard; Narita, Masashi; Piccirillo, Sara; Kenney-Herbert, Emma; Fawcett, James W.; Collins, V. Peter; Ichimura, Koichi; Watts, Colin

    2011-01-01

    Glioblastoma multiforme (GBM) is the most common type of primary brain tumor and a highly malignant and heterogeneous cancer. Current conventional therapies fail to eradicate or curb GBM cell growth. Hence, exploring the cellular and molecular basis of GBM cell growth is vital to develop novel therapeutic approaches. Neuroglia (NG)-2 is a transmembrane proteoglycan expressed by NG2+ progenitors and is strongly linked to cell proliferation in the normal brain. By using NG2 as a biomarker we id...

  2. NG2 targets tumorigenic Rb inactivation in Pit1-lineage pituitary cells.

    Science.gov (United States)

    Tateno, Toru; Nakano-Tateno, Tae; Ezzat, Shereen; Asa, Sylvia L

    2016-05-01

    The proteoglycan neuron-glial antigen 2 (NG2) is expressed by oligodendrocyte progenitors, pericytes, and some cancerous cells where it is implicated in tumor development. We examined mice with NG2-driven pRb inactivation. Unexpectedly, NG2-Cre:pRb(flox/flox) mice developed pituitary tumors with high penetrance. Adenohypophysial neoplasms developed initially as multifocal lesions; by 1 year, large tumors showed brain invasion. Immunohistochemistry identified these as Pit1-lineage neoplasms, with variable immunoreactivity for growth hormone, prolactin, thyrotropin, and α-subunit of glycoprotein hormones. Other than modest hyperprolactinemia, circulating hormone levels were not elevated. To determine the role of NG2 in the pituitary, we investigated NG2 expression. Immunoreactivity was identified in anterior and posterior lobes but not in the intermediate lobe of the mouse pituitary; in the adenohypophysis, folliculostellate cells had the strongest NG2 immunoreactivity but showed no proliferation in response to Rb inactivation. Pit1-positive adenohypophysial cells were positive for NG2, but corticotroph and gonadotroph cells were negative. RT-PCR revealed NG2 expression in normal human pituitary and human pituitary tumors; immunohistochemistry localized NG2 in nontumorous human adenohypophysis with strongest positivity in folliculostellate cells, and in tumors of all types except corticotrophs. Functional studies in GH4 mammosomatotrophs showed that NG2 increases prolactin (PRL), reduces growth hormone (GH) expression, and enhances cell adhesion without influencing proliferation. In conclusion, NG2-driven pRb inactivation results in pituitary tumors that mimic endocrinologically inactive Pit1-lineage human pituitary tumors. This model identifies a role for NG2 in pituitary cell-type-specific functions and unmasks a protective role from Rb inactivation in folliculostellate cells; it can be used for further research, including preclinical testing of novel therapies

  3. Histopathological findings in extended prostate biopsy with PSA ≤ 4 ng/mL

    Directory of Open Access Journals (Sweden)

    Katia R. Leite

    2008-06-01

    Full Text Available OBJECTIVE: Cancer detection has been reported in up to 27% of patients when lowering the PSA cutoff to 2.5 ng/mL. Although this practice could increase the number of biopsies performed, it also could lead to more frequent detection of significant prostate cancers at an organ-confined stage and/or a less aggressive state. This study describes the incidence of malignancy and tumor characteristics in extended prostate biopsies with PSA ≤ 4 ng/mL. MATERIALS AND METHODS: Prostate biopsies from 1081 patients where examined, 275 (25.4% patients had PSA level ≤ 4 ng/mL. RESULTS: Cancer was diagnosed in 32.0% and 35.7% of patients with PSA ≤ 4 ng/mL and > 4 ng/mL, respectively (p = 0.906. The median Gleason score was 7 independent of PSA > or ≤ 4 ng/mL (p = 0.078. The median number of cores positive for tumor was 4 and 3, respectively, for PSA > 4 ng/mL and PSA ≤ 4 ng/mL (p = 0.627. There was a difference in the total percent of tumors involving all cores, 11% and 7% for PSA > or ≤ 4 ng/mL (p = 0.042. Fifty-six patients underwent radical prostatectomy, 12 had PSA ≤ 4 ng/mL. In both groups, a diagnosis of cancer was accurate with no differences in Gleason score, tumor volume or staging for both groups. CONCLUSION: When PSA is below 4 ng/mL, cancer is detected in a proportion equal to the proportion diagnosed with a PSA > 4 ng/mL, and tumor characteristics are similar between the two groups. Only clinically significant tumors were diagnosed following radical prostatectomy.

  4. Polityka językowa Rumunii a dialekt csángó

    OpenAIRE

    Kozakiewicz, Joanna

    2012-01-01

    Abstract (Language policy of Romania and the dialect csángó). Csángós are an ethnic group of Hungarian origin, settled in Moldavian part of Romania at the end of 13th century. Over the centuries, they have lived in isolation which enabled preservation of their archaic dialect and culture. Today the bilingual Csángó speakers are on the verge of language shift to Romanian. The paper, apart from introducing the features of the endangered Csángó dialect, presents the relation between the Romanian...

  5. "Troonide mäng" toob fantaasia usutavalt teleekraanile / Mari Rebane

    Index Scriptorium Estoniae

    Rebane, Mari

    2011-01-01

    USA telekanali HBO telesarjast "Troonide mäng", George R. R. Martini "Jää ja tule laulu" sarja esimese raamatu "Troonide mäng" teleekraniseeringust. Sarja esimene osa esilinastus USA-s 17. aprillil. Osades Sean Bean jt.

  6. Kuuldemäng "Palju õnne argipäevaks!" edukümnes

    Index Scriptorium Estoniae

    2006-01-01

    Raadioteatri kuuldemäng "Palju õnne argipäevaks" jõudis Euroopa meediafestivalil Prix Europa kümne parema hulka. Näidendi lavastas raadio jaoks Andres Noormets. 1999. a. jõudis festivalil Prix Europa äramärkimiseni Ervin Õunapuu kuuldemäng "Õhtueine Emmauses"

  7. Axonal degeneration stimulates the formation of NG2+ cells and oligodendrocytes in the mouse

    DEFF Research Database (Denmark)

    Nielsen, Helle Hvilsted; Ladeby, Rune; Drøjdahl, Nina;

    2006-01-01

    Proliferation of the adult NG2-expressing oligodendrocyte precursor cells has traditionally been viewed as a remyelination response ensuing from destruction of myelin and oligodendrocytes, and not to the axonal pathology that is also a characteristic of demyelinating disease. To better understand...... the response of the NG2+ cells to the different components of demyelinating pathology, we investigated the response of adult NG2+ cells to axonal degeneration in the absence of primary myelin or oligodendrocyte pathology. Axonal degeneration was induced in the hippocampal dentate gyrus of adult mice...... by transection of the entorhino-dentate perforant path projection. The acutely induced degeneration of axons and terminals resulted in a prompt response of NG2+ cells, consisting of morphological transformation, cellular proliferation, and upregulation of NG2 expression days 2-3 after surgery. This was followed...

  8. Hur uppnås framgång på lång sikt med varumärkesutvidgningar? : En fallstudie av Hennes & Mauritz

    OpenAIRE

    Medanhodzic, Nejra; Karamehmedovic, Nadina

    2013-01-01

    Sammanfattning – ”Hur uppnås framgång på lång sikt med varumärkesutvidgningar?” Frågeställning: - Hur kan Hennes & Mauritz's varumärkesutvidgning studeras och beskrivas för att ett företag inom klädbranschen ska kunna ta del av denna information och lyckas utvidga framgångsrikt på lång sikt?- Hur ställer sig konsumenter mot utökningar av ett varumärke? Syfte:         Syftet med denna uppsats är att studera varumärkesutvidgning ur ett långsiktigt perspektiv genom att analysera företaget He...

  9. Kakaibang Halimuyak: Iba't Ibang Imahe ng Pangangapital sa Balintawak

    Directory of Open Access Journals (Sweden)

    Mariah Amour C. Miranda

    2015-06-01

    Full Text Available Ginagalugad ng sanaysay na ito ang isang pangunahing lugar na nadaraanan mula Norte patungong Maynila—ang Balintawak. Nagsisilbing manibela ng pagsusuri ang kontemporaneong pananaw sa lunan bilang pugad ng mga anyong may magkakasalungat na kahulugan. Binibigyang-pansin ng sanaysay ang iba't ibang imahe ng pagbebenta na matatagpuan sa Balintawak: ang billboard ng kumpanyang Bench na siyang pinakamalaki, ang mismong palengke, at ang mga lakong paninda sa tabing-kalsada. Sa pamamagitan nito, maipapakita ng sanaysay kung paano madadalumat ang Balintawak bilang isang panlipunang texto na sumasalamin sa mga magkakaibang pangangapital sa naturang lunan. This essay explores the space of Balintawak—a focal point of urban travel from northern Luzon to Manila. Using the contemporary view on space as a site of structures with contradictory meanings, the study seeks to understand how various images of market activities in Balintawak create meaningful representations. These images include the gigantic billboard of Bench, the central market itself, and flea markets in the area. The essay shows how Balintawak as a social text serves as a trading center of different forms of capital.

  10. Life Cycle GHG of NG-Based Fuel and Electric Vehicle in China

    OpenAIRE

    Qian Zhang; Xu Zhang; Xiliang Zhang; Xunmin Ou

    2013-01-01

    This paper compares the greenhouse gas (GHG) emissions of natural gas (NG)- based fuels to the GHG emissions of electric vehicles (EVs) powered with NG-to-electricity in China. A life-cycle model is used to account for full fuel cycle and use-phase emissions, as well as vehicle cycle and battery manufacturing. The reduction of life-cycle GHG emissions of EVs charged by electricity generated from NG, without utilizing carbon dioxide capture and storage (CCS) technology can be 36%–47% when co...

  11. Ang Social Network sa Facebook ng mga Taga-Batangas at ng mga Taga-Laguna: Isang Paghahambing

    Directory of Open Access Journals (Sweden)

    Jaderick P. Pabico

    2013-12-01

    Full Text Available Online social networking (OSN has become of great influence to Filipinos, where Facebook, Twitter, LinkedIn, Google+, and Instagram are among the popular ones. Their popularity, coupled with their intuitive and interactive use, allow one's personal information such as gender, age, address, relationship status, and list of friends to become publicly available. The accessibility of information from these sites allow, with the aid of computers, for the study of a wide population's characteristics even in a provincial scale. Aside from being neighbouring locales, the respective residents of Laguna and Batangas both derive their livelihoods from two lakes, Laguna de Bay and Taal Lake. Both residents experience similar problems, such as that, among many others, of fish kill. The goal of this research is to find out similarities in their respective online populations, particularly that of Facebook's. With the use of computational dynamic social network analysis (CDSNA, we found out that the two communities are similar, among others, as follows: both populations are dominated by single young female; Homophily was observed when choosing a friend in terms of age (i.e., friendships were created more often between people whose ages do not differ by at most five years; and Heterophily was observed when choosing friends in terms of gender (i.e., more friendships were created between a male and a female than between both people of the same gender. This paper also presents the differences in the structure of the two social networks, such as degrees of separation and preferential attachment.

  12. Klaaspärlimäng jätkub / Peeter Vähi

    Index Scriptorium Estoniae

    Vähi, Peeter, 1955-

    2005-01-01

    Marika Järvi ja Martin Kuuskmanni kontserdist 20. juulil (kaastegev Kanada pianist Matt Herskowitz) ja Euroopa Liidu Kammerorkesteri kontserdist 21. juulil Pärnu kontserdimajas muusikafestivali "Klaaspärlimäng" raames

  13. Merepõhjas lebavate vrakkide register muutub avalikuks / Risto Berendson ; kommenteerinud Enn Küng

    Index Scriptorium Estoniae

    Berendson, Risto, 1975-

    2010-01-01

    Eesti muinsuskaitseameti kavast uurida ja kaardistada Eesti merevetes uppunud laevavrakkide asukohad, millest luuakse avalik andmebaas. Kommentaari on lisanud Tartu Ülikooli Eesti ajaloo dotsent Enn Küng

  14. Generation of reactive astrocytes from NG2 cells is regulated by sonic hedgehog.

    Science.gov (United States)

    Honsa, Pavel; Valny, Martin; Kriska, Jan; Matuskova, Hana; Harantova, Lenka; Kirdajova, Denisa; Valihrach, Lukas; Androvic, Peter; Kubista, Mikael; Anderova, Miroslava

    2016-09-01

    NG2 cells, a fourth glial cell type in the adult mammalian central nervous system, produce oligodendrocytes in the healthy nervous tissue, and display wide differentiation potential under pathological conditions, where they could give rise to reactive astrocytes. The factors that control the differentiation of NG2 cells after focal cerebral ischemia (FCI) are largely unknown. Here, we used transgenic Cspg4-cre/Esr1/ROSA26Sortm14(CAG-tdTomato) mice, in which tamoxifen administration triggers the expression of red fluorescent protein (tomato) specifically in NG2 cells and cells derived therefrom. Differentiation potential (in vitro and in vivo) of tomato-positive NG2 cells from control or postischemic brains was determined using the immunohistochemistry, single cell RT-qPCR and patch-clamp method. The ischemic injury was induced by middle cerebral artery occlusion, a model of FCI. Using genetic fate-mapping method, we identified sonic hedgehog (Shh) as an important factor that influences differentiation of NG2 cells into astrocytes in vitro. We also manipulated Shh signaling in the adult mouse brain after FCI. Shh signaling activation significantly increased the number of astrocytes derived from NG2 cells in the glial scar around the ischemic lesion, while Shh signaling inhibition caused the opposite effect. Since Shh signaling modifications did not change the proliferation rate of NG2 cells, we can conclude that Shh has a direct influence on the differentiation of NG2 cells and therefore, on the formation and composition of a glial scar, which consequently affects the degree of the brain damage. GLIA 2016;64:1518-1531. PMID:27340757

  15. Chiral pharmacokinetics and inversion of NG-nitro-arginine in the rat

    Institute of Scientific and Technical Information of China (English)

    Yan-feiXIN; RuiTONG; YangFANG; Xiang-junZHOU; Yong-xiangWANG

    2004-01-01

    AIM: To explore pharmacokinetics of NG-nitro-D-arginine (D-NNA) and NG-nitro-L-arginine (L-NNA) in conscious rats.METHODS: The plasma concentration of D-NNA and L-NNA were determined by chiral ligand exchange method with capillary electrochromatography (CEC). Pharmacokinetic parameters were estimated using non-compartment model and were fitted using a computer program DAS. Chiral inversion rate of D-NNA to L-

  16. All "NG" Up On Clubs?: a "Bran-New Flawer" of Brennan-Flowers

    OpenAIRE

    Yew-Kwang Ng

    1981-01-01

    This article attempts to clarifv two controversial issues in the theory of clubs: (I) Abstract Is the Buchanan (Economica 1965) framework similar to the Tiebout (JPE 1956)? (2) Is the Pareto optimality condition of Ng(Economica 1973) generally applicable? Buchanan's own authoritv is invoked to give a negative answer to issue 1. A negative answer to issue 2 is refuted as being based on a misinterpreta tion of Ng's method of maximizing Pareto optimality or total net benefit ofthe whole populati...

  17. Extraordinarily high aldosterone, 901.0 ng/dL, in a patient with primary aldosteronism: an insight into the underlying mechanism.

    Science.gov (United States)

    Okubo, Yosuke; Sato, Yuka; Nakasone, Yasuto; Shirotori, Katsuko; Oguchi, Kazuhiro; Matsushita, Tsuyoshi; Nishikawa, Tetsuo; Yamazaki, Yuto; Sasano, Hironobu; Komatsu, Mitsuhisa; Yamauchi, Keishi; Aizawa, Toru

    2016-02-29

    A 43-yr-old hypertensive male was admitted due to hypokalemia (1.8 mEq/L) and renal dysfunction (eGFR, 20.0 mL/min/1.73 m(2)). His plasma aldosterone was 901.0 ng/dL, plasma renin activity 5.7 ng/mL/hr, and aldosterone/renin activity ratio 158. Angiotensin II (AII) was 0.7 pg/mL, ACTH mass spectrometry analysis showed that aldosterone (104 times the control) as well as its precursors were significantly elevated in the patient's plasma. A left adrenal (4-cm-diameter) tumor with (131)I-Adosterol® uptake was found and removed. Four days later, plasma aldosterone and renin activity had dropped to 7.73 ng/dL and 1.6 ng/mL/hr, respectively. However, they rose to 24.0 ng/dL and 10.9 ng/mL/hr, respectively, by Day 102. Nevertheless, magnetic resonance angiography found no evidence of a renovascular lesion. The tumor was a benign adrenocortical adenoma composed predominantly of clear cells positive for 17α-hydroxylase, [hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerases], and aldosterone synthase. A quantitative real-time polymerase chain reaction analysis of the tumor cells revealed that expression of the gene encoding aldosterone synthase was 85 times the control level. In addition, the tumor cells harbored G151R mutation of the inward rectifying potassium channel subfamily j, member 5 gene. The striking overexpression of aldosterone synthase by the tumor cells was considered the primary mechanism for the extravagant overproduction of aldosterone in this case. This overexpression may have resulted from integration of signals from AII and forced membrane depolarization due to the potassium channel mutation. PMID:26549209

  18. The novel hydroxylamine derivative NG-094 suppresses polyglutamine protein toxicity in Caenorhabditis elegans.

    Science.gov (United States)

    Haldimann, Pierre; Muriset, Maude; Vígh, László; Goloubinoff, Pierre

    2011-05-27

    Aggregation-prone polyglutamine (polyQ) expansion proteins cause several neurodegenerative disorders, including Huntington disease. The pharmacological activation of cellular stress responses could be a new strategy to combat protein conformational diseases. Hydroxylamine derivatives act as co-inducers of heat-shock proteins (HSPs) and can enhance HSP expression in diseased cells, without significant adverse effects. Here, we used Caenorhabditis elegans expressing polyQ expansions with 35 glutamines fused to the yellow fluorescent protein (Q35-YFP) in body wall muscle cells as a model system to investigate the effects of treatment with a novel hydroxylamine derivative, NG-094, on the progression of polyQ diseases. NG-094 significantly ameliorated polyQ-mediated animal paralysis, reduced the number of Q35-YFP aggregates and delayed polyQ-dependent acceleration of aging. Micromolar concentrations of NG-094 in animal tissues with only marginal effects on the nematode fitness sufficed to confer protection against polyQ proteotoxicity, even when the drug was administered after disease onset. NG-094 did not reduce insulin/insulin-like growth factor 1-like signaling, but conferred cytoprotection by a mechanism involving the heat-shock transcription factor HSF-1 that potentiated the expression of stress-inducible HSPs. NG-094 is thus a promising candidate for tests on mammalian models of polyQ and other protein conformational diseases. PMID:21471208

  19. Life Cycle GHG of NG-Based Fuel and Electric Vehicle in China

    Directory of Open Access Journals (Sweden)

    Qian Zhang

    2013-05-01

    Full Text Available This paper compares the greenhouse gas (GHG emissions of natural gas (NG- based fuels to the GHG emissions of electric vehicles (EVs powered with NG-to-electricity in China. A life-cycle model is used to account for full fuel cycle and use-phase emissions, as well as vehicle cycle and battery manufacturing. The reduction of life-cycle GHG emissions of EVs charged by electricity generated from NG, without utilizing carbon dioxide capture and storage (CCS technology can be 36%–47% when compared to gasoline vehicles. The large range change in emissions reduction potential is driven by the different generation technologies that could in the future be used to generate electricity in China. When CCS is employed in power plants, the GHG emission reductions increase to about 71%–73% compared to gasoline vehicles. It is found that compressed NG (CNG and liquefied NG (LNG fuels can save about 10% of carbon as compared to gasoline vehicles. However, gas-to-liquid (GTL fuel made through the Fischer-Tropsch method will likely lead to a life-cycle GHG emissions increase, potentially 3%–15% higher than gasoline, but roughly equal to petroleum-based diesel. When CCS is utilized, the GTL fueled vehicles emit roughly equal GHG emissions to petroleum-based diesel fuel high-efficient hybrid electric vehicle from the life-cycle perspective.

  20. SNOW AVALANCHE ACTIVITY IN PARÂNG SKI AREA REVEALED BY TREE-RINGS

    Directory of Open Access Journals (Sweden)

    F. MESEȘAN

    2014-11-01

    Full Text Available Snow Avalanche Activity in Parâng Ski Area Revealed by Tree-Rings. Snow avalanches hold favorable conditions to manifest in Parâng Mountains but only one event is historically known, without destructive impact upon infrastructure or fatalities and this region wasn’t yet the object of avalanche research. The existing ski infrastructure of Parâng resort located in the west of Parâng Mountains is proposed to be extended in the steep slopes of subalpine area. Field evidence pinpoints that these steep slopes were affected by snow avalanches in the past. In this study we analyzed 11 stem discs and 31 increment cores extracted from 22 spruces (Picea abies (L. Karst impacted by avalanches, in order to obtain more information about past avalanches activity. Using the dendrogeomorphological approach we found 13 avalanche events that occurred along Scărița avalanche path, since 1935 until 2012, nine of them produced in the last 20 years. The tree-rings data inferred an intense snow avalanche activity along this avalanche path. This study not only calls for more research in the study area but also proves that snow avalanches could constitute an important restrictive factor for the tourism infrastructure and related activities in the area. It must be taken into consideration by the future extension of tourism infrastructure. Keywords: snow avalanche, Parâng Mountains, dendrogeomorphology, ski area.

  1. TIẾP CẬN NHÂN HỌC TRONG NGHIÊN CỨU VỀ NGUỒN GỐC CƯ DÂN HUẾ VÀ VÙNG PHỤ CẬNNHỮNG MẢNG MÀU LÀM NÊN SẮC THÁI VĂN HÓA VÙNG ĐẤT NÚI NGỰ SÔNG HƯƠNG

    OpenAIRE

    Nguyen Xuan, Hong; Nguyễn Xuân, Hồng

    2010-01-01

    VĂN HÓA - LỊCH SỬ HUẾ QUA GÓC NHÌN LÀNG XÃ PHỤ CẬN VÀ QUAN HỆ VỚI BÊN NGOÀI Session 2: Văn hoá - lịch sử Huế và phụ cận フエの文化と歴史:周辺集落と外部との関係からの視点より Session 2: Culture - history of Hue and its surroundings フエとその周辺の文化・歴史

  2. Noble Gas Inserted Protonated Silicon Monoxide Cations: HNgOSi(+) (Ng = He, Ne, Ar, Kr, and Xe).

    Science.gov (United States)

    Sekhar, Pooja; Ghosh, Ayan; Ghanty, Tapan K

    2015-11-25

    The existence of noble gas containing protonated silicon monoxide complexes have been predicted theoretically through ab initio quantum chemical methods. The predicted HNgOSi(+) ions are obtained by insertion of a noble gas atom (Ng = He, Ne, Ar, Kr, and Xe) between the H and O atoms in SiOH(+) ion. The structural parameters, energetics, harmonic vibrational frequencies, and charge distributions have been analyzed by optimizing the minima and the transition state structures using second-order Møller-Plesset perturbation theory (MP2), density functional theory (DFT), and coupled-cluster theory (CCSD(T)) based techniques. The predicted HNgOSi(+) ions are found to be stable with respect to all possible 2-body and 3-body dissociation channels, except the dissociation path leading to the respective global minimum products. However, these ions are found to be kinetically stable with respect to the global minimum dissociation process as revealed from the finite barrier heights, which in turn can prevent the transformation of these metastable species to the global minimum products. Furthermore, the computed bond lengths, vibrational frequencies, and force constant values suggest that a strong covalent bond exists between the H and Ng atoms in HNgOSi(+) ions while the Ng and O atoms share a strong van der Waals kind of interaction. Charge distributions and bonding analysis indicate that HNgOSi(+) ions can be best represented as strong complexes between the [HNg](+) ions and OSi molecule. All the computational results suggest that the predicted species, HNgOSi(+), may be prepared and characterized by suitable experimental technique at cryogenic temperature. PMID:26501440

  3. Is extended biopsy protocol justified in all patients with PSA ≥ 20 ng/mL?

    Institute of Scientific and Technical Information of China (English)

    Xiaojun Deng; Jian Chu; Bo Yang; Feng Liu; Weifeng Wang; Jidong Hao; Jiansheng Wan; Hui Liu

    2014-01-01

    Objective: The aim of this study was to investigate whether it was necessary to increase the number of cores at initial prostate biopsy with patients of prostate-specific antigen (PSA) ≥ 20 ng/mL and to explore an appropriate individual-ized transrectal ultrasonograhpy (TRUS)-guided prostate biopsy for the detection of prostate cancer in men suspicious of prostate cancer.Methods:A total of 115 patients with PSA ≥ 20 ng/mL and suspicious of prostate cancer were prospectively randomized to perform TRUS-guided biopsy. Patients were randomized to a “6 + X” cores or a “10 + X” cores protocol. The primary end point was cancer detection rate. Secondary end points were cancer characteristics, rate of complications and the level of pain experienced by patients during TRUS-guided prostate biopsy.Results:Preoperative variables were similar in both groups. The overal prostate cancer detection rate was 73.9%. The “10 + X” cores strategy increased cancer detection rate only 9.7% in patients with PSA ≥ 20 ng/mL but < 50 ng/mL, while there was no diference between the two strategies for cancer detection in patients with PSA ≥ 50.1 ng/mL. The number of extended biopsy cores and pain score of extended biopsy in prostate cancer patients increased significantly (P < 0.001).Conclusion:Our findings suggest that there is no significant advantage in using extended biopsy protocol in al patients with PSA ≥ 20 ng/mL.

  4. Examination of steam generator alloy 800 NG tube from the Almaraz unit 2 NPP

    International Nuclear Information System (INIS)

    The steam generators of Almaraz Unit 2 were replaced in 1997 by the model 61W/D3 (Siemens) with Alloy 800NG steam generator tubes. Denting indications were firstly detected in 2006 in the SG-3. Crack indications were identified in 2009. At the end of 2011, three tubes were recovered from this steam generator to carry out destructive examination in order to identify the root cause of the tubes degradation. Analysis of deposits point out the existence of multiples elements in the removed OD (Outer Diameter) deposits as well as in the deposits at the free tube under sludge and at the transition zone. Deposits are more abundant at the transition zone than at free tube. About 10% Na concentration has been detected, whereas S and Cl appear in small concentrations. Si appears regularly and Cr, Ni concentrations in the deposits are similar. Multiple intergranular cracks have been detected at 3 mm above the last contact point between the tube and the TS (tube support), in a band of around 5 mm, practically in the whole perimeter of the tube. Fracture surface of crack-B was partially covered by a Si rich layer, whereas fracture surface of crack-A seems to be cleaner. However, no significant differences in composition, except higher amount of S in crack-B, were found in the deposits of both cracks. EDX mapping and Auger profiles point out Ni enrichment with slight Cr enrichment or depletion and Fe depletion. The comparison of Auger profiles with available results for Alloy 800 tested in caustic and acid sulfate environments seems to indicate that the environment inside the cracks detected in the tube R67C48 is neutral or moderately caustic

  5. Hans Küng se voorstel vir ’n eietydse etiek

    Directory of Open Access Journals (Sweden)

    S. A. Strauss

    1998-06-01

    Full Text Available This contribution undertakes a critical assessment of the well-known Hans Küng's proposal for contemporary ethics in his book Global responsibility. In search of a new world ethic (1990. This article commences by providing some background on this publication as well as on the theology of Küng in general. The content of the book is then paraphrased in summarised form. The last part of the article analyses four major convictions of Kung, against which the Reformed doctrine is brought forward. These convictions deal with the ethical aspects of normativity, humanity, postmodernity and ecumenicity.

  6. The curious case of NG2 cells: transient trend or game changer?

    Directory of Open Access Journals (Sweden)

    Vittorio Gallo

    2011-03-01

    Full Text Available It has been 10 years since the seminal work of Dwight Bergles and collaborators demonstrated that NG2 (nerve/glial antigen 2-expressing oligodendrocyte progenitor cells (NG2 cells receive functional glutamatergic synapses from neurons (Bergles et al., 2000, contradicting the old dogma that only neurons possess the complex and specialized molecular machinery necessary to receive synapses. While this surprising discovery may have been initially shunned as a novelty item of undefined functional significance, the study of neuron-to-NG2 cell neurotransmission has since become a very active and exciting field of research. Many laboratories have now confirmed and extended the initial discovery, showing for example that NG2 cells can also receive inhibitory GABAergic synapses (Lin and Bergles, 2004 or that neuron-to-NG2 cell synaptic transmission is a rather ubiquitous phenomenon that has been observed in all brain areas explored so far, including white matter tracts (Kukley et al., 2007; Ziskin et al., 2007; Etxeberria et al., 2010. Thus, while still being in its infancy, this field of research has already brought many surprising and interesting discoveries, and has become part of a continuously growing effort in neuroscience to re-evaluate the long underestimated role of glial cells in brain function (Barres, 2008. However, this area of research is now reaching an important milestone and its long-term significance will be defined by its ability to uncover the still elusive function of NG2 cells and their synapses in the brain, rather than by its sensational but transient successes at upsetting the old order established by neuronal physiology. To participate in the effort to facilitate such a transition, here we propose a critical review of the latest findings in the field of NG2 cell physiology – discussing how they inform us on the possible function(s of NG2 cells in the brain – and we present some personal views on new directions the field could

  7. Regulation of N-CAM and ganglioside expression in neuroblastoma x glioma hybrid, NG108-15, cells

    International Nuclear Information System (INIS)

    A monoclonal antibody (Mab) that recognizes the neural cell-adhesion sialoglycoprotein, N-CAM, and another Mab, A2B5, that recognizes a family of gangliosides were used to study the regulation of their expression in NG108-15 cells. Radioimmunoassay of membrane fractions or analysis of western blots of solubilized proteins with 125I-labelled antibodies was performed. Retinoic acid (1.6 μM: 48 hr) and the potent tumor promoter, PMA (0.16 μM: 48 hr), were found to elevate N-CAM and ganglioside levels 2-3 fold over controls. Under the same conditions, phorbol alone had no effect but dibutyrl-cyclic AMP (1 mM) was found to inhibit N-CAM expression (by 50%) but stimulate A2B5 binding 2-fold. When NG108-15 cells were cocultured with fetal rat muscle, muscle conditioned medium or in the presence of exudates from muscle cultures, marked effects on the expression of the different molecular weight forms of N-CAM were observed. These results further support the idea that cell surface sialoglycoconjugate expression is under complex control, perhaps involving the regulation of glycosyltransferases by cyclic-AMP-dependent protein kinase and protein kinase C

  8. Multi-analyte approach for the determination of ng L-1 levels of steroid hormones in unidentified aqueous samples

    International Nuclear Information System (INIS)

    Since the 1970s, many analytical methods for the detection of illegal growth promoters, such as thyreostats, anabolics, β-agonists and corticosteroids have been developed for a wide range of matrices of animal origin, including meat, fat, organ tissue, urine and faeces. The aim of this study was to develop an analytical method for the determination of ng L-1 levels of estrogens, gestagens, androgens (EGAs) and corticosteroids in aqueous preparations (i.e. drinking water, drinking water supplements), commercially available on the 'black' market. For this, extraction was performed with Bakerbond C18 speedisk, a technique commonly used in environmental analysis. After fractionation, four fractions were collected using a methanol:water gradient program. Gas chromatography coupled to electron impact multiple mass spectrometry (GC-EI-MS2) screening for the EGAs was carried out on the derivatized extracts. For the detection of corticosteroids, gas chromatography coupled to negative chemical ionization mass spectrometry (GC-NCI-MS) was used after oxidation of the extracts. Confirmation was done by liquid chromatography coupled to electrospray ionization multiple mass spectrometry (LC-ESI-MS2). The combined use of GC and LC coupled to MS enabled the identification and quantification of anabolics and corticosteroids at the low ng L-1 level. This study demonstrated the occurrence of both androgens and corticosteroids in different commercial aqueous samples

  9. Multi-analyte approach for the determination of ng L(-1) levels of steroid hormones in unidentified aqueous samples.

    Science.gov (United States)

    Noppe, H; Verheyden, K; Gillis, W; Courtheyn, D; Vanthemsche, P; De Brabander, H F

    2007-03-14

    Since the 1970s, many analytical methods for the detection of illegal growth promoters, such as thyreostats, anabolics, beta-agonists and corticosteroids have been developed for a wide range of matrices of animal origin, including meat, fat, organ tissue, urine and faeces. The aim of this study was to develop an analytical method for the determination of ng L(-1) levels of estrogens, gestagens, androgens (EGAs) and corticosteroids in aqueous preparations (i.e. drinking water, drinking water supplements), commercially available on the 'black' market. For this, extraction was performed with Bakerbond C18 speedisk, a technique commonly used in environmental analysis. After fractionation, four fractions were collected using a methanol:water gradient program. Gas chromatography coupled to electron impact multiple mass spectrometry (GC-EI-MS2) screening for the EGAs was carried out on the derivatized extracts. For the detection of corticosteroids, gas chromatography coupled to negative chemical ionization mass spectrometry (GC-NCI-MS) was used after oxidation of the extracts. Confirmation was done by liquid chromatography coupled to electrospray ionization multiple mass spectrometry (LC-ESI-MS2). The combined use of GC and LC coupled to MS enabled the identification and quantification of anabolics and corticosteroids at the low ng L(-1) level. This study demonstrated the occurrence of both androgens and corticosteroids in different commercial aqueous samples. PMID:17386692

  10. 76 FR 72087 - Special Conditions: Diamond Aircraft Industries, Model DA-40NG; Electronic Engine Control (EEC...

    Science.gov (United States)

    2011-11-22

    ... powerplant control systems.'' The overall intent of the above cited rules is to provide a robust and fault... radiated frequency (HIRF). Testing to a level lower than required for a catastrophic failure results in a...-40NG; Electronic Engine Control (EEC) System AGENCY: Federal Aviation Administration (FAA), DOT....

  11. Lavale jõuab näitemäng armastuse orjast

    Index Scriptorium Estoniae

    2007-01-01

    Komöödiateatril tuleb lavale Jaan Toominga lavastatud "Venuse armumäng" austria kirjaniku Leopold von Sacher-Masochi romaani "Venus karusnahas" järgi. Osades Veikko Täär, Britta Vahur ja kulturist Imre Vähi

  12. Puuduv lugu, ainult kangelane : Zidane ja kunsti mäng / Zoe Stillpass

    Index Scriptorium Estoniae

    Stillpass, Zoe

    2010-01-01

    Režissööride Philippe Parreno ja Douglas Gordoni film prantsuse jalgpallurist Zinedine Zidanest. Film on 23. aprillil 2005 peetud mäng Real Madridi ja Villareali vahel Madridis, mida filmiti 17 kaameraga, mis kõik olid fokusseeritud Zidane'ile. Helitaust pärineb šoti alternatiivroki bändilt Mogwai

  13. Tiềm năng lớn của vệ tinh siêu nhỏ đáp ứng nhu cầuquản lý hàng hải của Việt Nam

    OpenAIRE

    Vu Trong, Thu; Pham Quang, Hung; Nguyen Hoang, Giang; Nguyen, Hugo

    2011-01-01

    Trong 10 năm gần đây, loại vệ tinh siêu nhỏ có kích thước 10x10x10 cm và trọng lượng 1 kg với tên gọi CubeSat từ công cụ thực hành của sinh viên đại học đang dần trở thành nền tảng cho những vệ tinh có khả năngng dụng trong thực tiễn. Ngày nay, người sử dụng có thể tự thiết kế hoặc mua sẵn linh kiện điện tử và cơ khí lắp thành vệ tinh cỡ 1, 2, 3... kg với các tính năng mong muốn. Ưu điểm của loại vệ tinh siêu nhỏ này là thời gian chế tạo ngắn, chi phí thấp và càng phát huy năng lực khi sử d...

  14. Two separate metalloproteinase activities are responsible for the shedding and processing of the NG2 proteoglycan in vitro.

    Science.gov (United States)

    Asher, Richard A; Morgenstern, Daniel A; Properzi, Francesca; Nishiyama, Akiko; Levine, Joel M; Fawcett, James W

    2005-05-01

    A high proportion of NG2 in the adult rat spinal cord is saline-soluble and migrates slightly faster than intact NG2 on SDS-PAGE, suggesting that it represents the shed ectodomain of NG2. In the injured cerebral cortex, much of the overall increase in NG2 is due to the saline-soluble (shed), rather than the detergent-soluble (intact), form. Hydroxamic acid metalloproteinase inhibitors, but not TIMPs, were able to prevent NG2 shedding in oligodendrocyte precursor cells (OPCs) in vitro. The generation of another truncated form of NG2 was, however, sensitive to TIMP-2 and TIMP-3. Two observations suggest that NG2 is involved in PDGF signaling in OPCs: the rate of NG2 shedding increased with cell density and NG2 expression was increased in the absence of PDGF. Ectodomain shedding converts NG2 into a diffusible entity able to interact with the growth cone, and we suggest that this release is likely to enhance its axon growth-inhibitory activity. PMID:15866049

  15. Straightforward strategy for quantifying rotundone in wine at ngL(-1) level using solid-phase extraction and gas chromatography-quadrupole mass spectrometry. Occurrence in different varieties of spicy wines.

    Science.gov (United States)

    Culleré, Laura; Ontañón, Ignacio; Escudero, Ana; Ferreira, Vicente

    2016-09-01

    This paper presents a straightforward methodology to quantify rotundone in wine at ngL(-1) level. This compound, responsible for the black pepper aromatic note, may have sensorial relevance in some wines due to its low odor threshold, estimated at only 16ngL(-1) in red wines. The proposed strategy is based on solid phase extraction and analysis by GC-MS. The detection limit value was 0.6ngL(-1), which is more than one order of magnitude below its odor threshold in wine. Matrix effects have not been found and a synthetic wine calibration was proposed. The precision of the method was evaluated in reproducibility terms, obtaining a very acceptable value (RSD 4%). The optimized and validated strategy was applied to quantify this molecule in thirty wines belonging to different varieties as Graciano, Maturana tinta, Schioppettino, Shiraz, Duras and Gamay. Two of these wines exhibited levels higher than 100ngL(-1). PMID:27041325

  16. Characterization of cells recovered from the xenotransplanted NG97 human-derived glioma cell line subcultured in a long-term in vitro

    Directory of Open Access Journals (Sweden)

    Heinrich Juliana K

    2008-10-01

    Full Text Available Abstract Background In order to elucidate tumoral progression and drug resistance, cultured cell lines are valuable tools applied on tumor related assays provided they are well established and characterized. Our laboratory settled the NG97 cell line derived from a human astrocytoma grade III, which started to develop and express important phenotypical characteristics of an astrocytoma grade IV after injection in the flank of nude mice. Astrocytomas are extremely aggressive malignancies of the Central Nervous System (CNS and account for 46% of all primary malignant brain tumors. Progression to worse prognosis occurs in 85% of the cases possibly due to changes in cell tumor microenvironment and through biological pathways that are still unclear. Methods This work focused on characterizing the NG97 cell line specifically after being recovered from the xenotransplant, who maintained their undifferentiated characteristics along the following 60th passages in vitro. These cells were subcultivated to evaluate the possible contribution of these undifferentiated characteristics to the malignant progression phenotype. These characteristics were the expression of molecules involved in the processes of migration, dedifferentiation and chromosomal instability. Results Results showed that NG97(ht had an decrease in doubling time through sub cultivation, which was characterized by a converse modulation between the expression of glial fibrillary acidic protein (GFAP and vimentin. In addition, β1 integrins were present in intermediate levels while α5 integrins had a high expression profile as well as fibronectin and laminin. Cytogenetic analysis of NG97(ht revealed several chromosomal abnormalities, 89% of the cells showed to be hyperdiploid and the modal number was assigned to be 63. Several acrocentric chromosomes were visualized and at least 30 figures were attributed to be murine. These findings suggest a possible fusion between the original NG97 cells

  17. Localisation of NG2 immunoreactive neuroglia cells in the rat locus coeruleus and their plasticity in response to stress

    Directory of Open Access Journals (Sweden)

    Mohsen eSeifi

    2014-05-01

    Full Text Available The locus coeruleus (LC nucleus modulates adaptive behavioural responses to stress and dysregulation of LC neuronal activity is implicated in stress-induced mental illnesses. The LC is composed primarily of noradrenergic neurons together with various glial populations. A neuroglia cell-type largely unexplored within the LC is the NG2 cell. NG2 cells serve primarily as oligodendrocyte precursor cells throughout the brain. However, some NG2 cells are in synaptic contact with neurons suggesting a role in information processing. The aim of this study was to neurochemically and anatomically characterise NG2 cells within the rat LC. Furthermore, since NG2 cells have been shown to proliferate in response to traumatic brain injury, we investigated whether such NG2 cells plasticity also occurs in response to emotive insults such as stress. Immunohistochemistry and confocal microscopy revealed that NG2 cells were enriched within the pontine region occupied by the LC. Close inspection revealed that a sub-population of NG2 cells were located within unique indentations of LC noradrenergic somata and were immunoreactive for the neuronal marker NeuN whilst NG2 cell processes formed close appositions with clusters immunoreactive for the inhibitory synaptic marker proteins gephyrin and the GABA-A receptor alpha3-subunit, on noradrenergic dendrites. In addition, LC NG2 cell processes were decorated with vesicular glutamate transporter 2 immunoreactive puncta. Finally, ten days of repeated restraint stress significantly increased the density of NG2 cells within the LC. The study demonstrates that NG2 IR cells are integral components of the LC cellular network and they exhibit plasticity as a result of emotive challenges.

  18. Návrh zatahovacího podvozku pro letoun NG4

    OpenAIRE

    BARTONĚK, Jaroslav

    2011-01-01

    Diplomová práce popisuje návrh způsobu zatahování podvozku pro ultralehký letoun NG-4. Jejími hlavními částmi jsou popis všeobecného principu zatahování, návrh kinematiky, výpočet zatížení a pevnostní kontrola, volba potřebných materiálů a způsob ovládání podvozku pilotem. Okrajovou náplní práce je členění letadlových podvozků a jejich historický vývoj. This diploma thesis describes the design of retractable landing gear for the NG-4 ultralight aircraft. Its main part consists of general p...

  19. La oración simple ngäbére

    Directory of Open Access Journals (Sweden)

    Murillo Miranda José Manuel

    2010-06-01

    Full Text Available Este artículo hace una descripción inicial de las características más importantes de la oración simple de la variante de la lengua ngäbére (guaymí hablada en el Pacífico Sur de Costa Rica. Sin embargo, aunque no es un trabajo comparativo propiamente dicho, sí se hacen unas pequeñas menciones de las propiedades de la oración simple en el ngäbére panameño, sobre el cual existen un poco más de estudios.

  20. The NG2 Proteoglycan Protects Oligodendrocyte Precursor Cells against Oxidative Stress via Interaction with OMI/HtrA2.

    Directory of Open Access Journals (Sweden)

    Frank Maus

    Full Text Available The NG2 proteoglycan is characteristically expressed by oligodendrocyte progenitor cells (OPC and also by aggressive brain tumours highly resistant to chemo- and radiation therapy. Oligodendrocyte-lineage cells are particularly sensitive to stress resulting in cell death in white matter after hypoxic or ischemic insults of premature infants and destruction of OPC in some types of Multiple Sclerosis lesions. Here we show that the NG2 proteoglycan binds OMI/HtrA2, a mitochondrial serine protease which is released from damaged mitochondria into the cytosol in response to stress. In the cytosol, OMI/HtrA2 initiates apoptosis by proteolytic degradation of anti-apoptotic factors. OPC in which NG2 has been downregulated by siRNA, or OPC from the NG2-knockout mouse show an increased sensitivity to oxidative stress evidenced by increased cell death. The proapoptotic protease activity of OMI/HtrA2 in the cytosol can be reduced by the interaction with NG2. Human glioma expressing high levels of NG2 are less sensitive to oxidative stress than those with lower NG2 expression and reducing NG2 expression by siRNA increases cell death in response to oxidative stress. Binding of NG2 to OMI/HtrA2 may thus help protect cells against oxidative stress-induced cell death. This interaction is likely to contribute to the high chemo- and radioresistance of glioma.

  1. LA FRASE NOMINAL NGÄBÉRE The Nominal Phrase Ngäbére

    Directory of Open Access Journals (Sweden)

    José Manuel Murillo Miranda

    2009-12-01

    Full Text Available Este trabajo es el avance de una amplia investigación cuyo propósito es elaborar una gramática descriptiva de la lengua chibcha ngäbére. El artículo presenta las características particulares más relevantes de la frase nominal de dicha variante dialectal (hablada en Costa Rica, también en Panamá, y establece la relación con algunas de las características generales de otras lenguas chibchas ístmicas. El primer apartado del escrito es una introducción a la lengua ngäbére y a sus hablantes; el segundo presenta la morfología de los sustantivos, pronombres y otras clases de palabras que conforman la frase nominal; el tercer apartado trata de la sintaxis de la frase nominal y esboza, de manera sucinta, las relaciones gramaticales; el cuarto apartado es una conclusión valorativa de este trabajo.This work is a preview of a broader study whose goal is to create a descriptive grammar of the Chibchan language, Ngäbere. The article describes the most relevant features of the dialect's nominal phrase (spoken in Costa Rica and Panama and establishes the relationship with some of the general features of other Isthmian Chibchan languages. The first part of the report is an introduction to Ngäbere and it speakers; the second, describes the morphology of the nouns, pronouns, and other words that make up the nominal phrase; the third, is about the syntax of the nominal phrase and it outlines in a succinct manner, grammatical relationships; the last part is a critical conclusion of this work.

  2. Study of neurotoxicity and neuroprotectivity using NG 108-15 cells

    Czech Academy of Sciences Publication Activity Database

    Borovičková, Lenka; Šloncová, Eva; Slaninová, Jiřina

    Praha : ÚOCHB AV ČR, 2003 - (Slaninová, J.), s. 4-6 ISBN 80-86241-20-3. [Biologically Active Peptides /8./. Praha (CZ), 23.04.2003-25.04.2003] R&D Projects: GA ČR GA305/03/1100 Institutional research plan: CEZ:AV0Z5052915; CEZ:AV0Z4055905 Keywords : Alzheimer's disease * humanin * NG 108-15 cells Subject RIV: CE - Biochemistry

  3. Phenotype overlap in glial cell populations: astroglia, oligodendroglia and NG-2(+ cells

    Directory of Open Access Journals (Sweden)

    Robert eFern

    2015-05-01

    Full Text Available The extent to which NG-2(+ cells form a distinct population separate from astrocytes is central to understanding whether this important cell class is wholly an oligodendrocyte precursor cell (OPC or has additional functions akin to those classically ascribed to astrocytes. Early immuno-staining studies indicate that NG-2(+ cells do not express the astrocyte marker GFAP, but orthogonal reconstructions of double-labelled confocal image stacks here reveal a significant degree of co-expression in individual cells within post-natal day 10 (P10 rat optic nerve (RON and rat cortex. Extensive scanning of various antibody/fixation/embedding approaches identified a protocol for selective post-embedded immuno-gold labelling. This first ultrastructural characterization of identified NG-2(+ cells revealed populations of both OPCs and astrocytes in P10 RON. NG-2(+ astrocytes had classic features including the presence of glial filaments but low levels of glial filament expression were also found in OPCs and myelinating oligodendrocytes. P0 RONs contained few OPCs but positively identified astrocytes were observed to ensheath pre-myelinated axons in a fashion previously described as a definitive marker of the oligodendrocyte lineage. Astrocyte ensheathment was also apparent in P10 RONs, was absent from developing nodes of Ranvier and was never associated with compact myelin. Astrocyte processes were also shown to encapsulate some oligodendrocyte somata. The data indicate that common criteria for delineating astrocytes and oligodendroglia are insufficiently robust and that astrocyte features ascribed to OPCs are likely to arise from misidentification.

  4. Parada ng Lechon in Balayan, Batangas Philippines to Honor St. John the Baptist: An Ethnographic Study

    Directory of Open Access Journals (Sweden)

    Dr. Maria Luisa A. Valdez

    2014-06-01

    Full Text Available Thisstudy generally aimed to document the existing practice of the “Parada ng Lechon” in Balayan, Batangas Philippines to honor St. John the Baptist and the implications of the findings in relation to the study of Philippine culture. This paper employed the ethnographic research method which involved the use of documentary materials, participant observation method, questionnaires, and interviews with 150 purposively selected respondents. The results of the study revealed the world-famous “Parada ng Lechon” which originated as an old thanksgiving custom of the working class in what-used-to-be the poor and depressed area of the western district of Balayan, Batangas, Philippines. It was noteworthy to mention that during the Spanish and American regimes, families who were fortunate enough to receive some significant blessings during the past year would parade a lechon in the town plaza every June 24 - the Feast of St. John the Baptist. For the Balayeños, the parading of lechon is the best expression of thanksgiving and veneration to their patron saint. Even during these times, the sight of people parading lechons in Balayan - coupled with centuries-old practice of water dousing - was quite a spectacle to behold. The “Parada ng Lechon” is considered an invaluable asset that encapsulates the Philippine culture which may be cherished for posterity.

  5. Health Information in Vietnamese (Tiếng Việt): MedlinePlus

    Science.gov (United States)

    ... Health Services Administration Child Dental Health Early Childhood Caries - English Chứng Sâu Răng Sớm ở Trẻ Em - ... Việt (Vietnamese) Bilingual PDF Health Information Translations Diabetic Diet Diabetes Meal Plan Basics Hoaïch Ñònh Böõa AÊn ...

  6. Health Information in Vietnamese (Tiếng Việt): MedlinePlus

    Science.gov (United States)

    ... Việt (Vietnamese) Bilingual PDF Health Information Translations Postpartum Depression Emotional Changes After Giving Birth Thay Đổi Cảm Xúc Sau Khi Sanh Con - Tiếng Việt (Vietnamese) Bilingual PDF Health Information Translations Pregnancy A Healthy Pregnancy Mang Thai Khỏe Mạnh - Tiếng ...

  7. Effects of NG-nitro-L-arginine on focal cerebral ischemic injury in rats

    Institute of Scientific and Technical Information of China (English)

    Jianxin Zhang; Huixin Zhang; Lanfang Li; Yonghui Li

    2006-01-01

    BACKGROUND: Previous researches have proved that aminoguanidin can cure cerebral ischemic injury remarkably as a selective induced nitricoxide synthase (iNOS) inhibitor. However, whether nonselective NOS inhibitor could protect cerebral ischemic injury or not is unclear.OBJECTIVE: To investigate the effects of NG-nitro-L-arginine (L-NA), a nonselective nitricoxide synthase (NOS) inhibitor, on cerebral ischemic injury of rats and the possible mechanism.DESIGN: Randomized controlled study.SETTING: Pharmacological Department of Medical Academy of Science of Hebei Province.MATERIALS: A total of 56 male healthy SD rats, of grade II, weighting 250-290 g, were provided by the Experimental Animal Center of Hebei Province (certification: 04036).METHODS: The experiment was completed in the Pharmacological Department of Medical Academy of Science of Hebei Province from March 2005 to January 2006. ① Grouping: Rats were randomly divided into 3 groups: sham operation group (n=8), model group (n=24) and L-NA group (n=24). ② Modeling:Middle cerebral artery (MCA) was established on rats in model group and L-NA group with intraluminal line occlusion methods, and rats in sham operation group were separated their external carotid arteries without occlusion of internal carotid artery. ③ Intervention study: Rats in model group and L-NA group were injected intraperitoneally with 10 mL/kg and 20 mg/kg L-NA at 2, 6 and 12 hours respectively after ischemia twice a day for 3 consecutive days. ④ Rats were sacrificed on the third day for measuring volume of cerebral infarction with image analysis and swelling degrees and activities of mitochondria with electron microscope. Effect of L-NA on ultrastructural changes of neurons in cortex was observed after ischemia.MAIN OUTCOME MEASURES: ① Volume of cerebral infarction; ② Swelling degrees, contents of nitric oxide (NO) and malondialdehyde (MDA) and activities of adenosine triphosphatase (ATPase), superoxide dismutase (SOD) and

  8. Frenkel-Gross' irregular connection and Heinloth-Ngô-Yun's are the same

    OpenAIRE

    Zhu, Xinwen

    2012-01-01

    We show that the irregular connection on G_m constructed by Frenkel-Gross (2009) and the one constructed by Heinloth-Ngô-Yun (arXiv:1005.2765) are the same, which confirms the Conjecture 2.14 of arXiv:1005.2765. The proof is simple, modulo the big machinery of quantization of Hitchin’s integrable systems as developed by Beilinson-Drinfeld ([BD]). The idea is as follows. Let E be the irregular connection on G_m as constructed by Frenkel-Gross. It admits a natural oper form. We apply the...

  9. Implementation of Order-wire Functionality in IRITEL NG-SDH devices

    Directory of Open Access Journals (Sweden)

    D. Katanić

    2011-06-01

    Full Text Available This paper describes the implementation of order-wire functionality in NG-SDH devices ODS2G5 and OTS622 IRITEL. The paper also describes the operation of order-wire in networks with arbitrary topologies, as well as the configuration of order-wire functionality by using network management software SUNCE. The presented solution for order-wire functionality is compared with VoIP solutions. The paper shows that the presented solution provides fast reaction to network failures, while keeping the implementation simple.

  10. NC/NG与ADN的相互作用%Interaction of NG/NC with ADN

    Institute of Scientific and Technical Information of China (English)

    张腊莹; 衡淑云; 刘子如; 张皋; 赵凤起; 谭惠民

    2009-01-01

    用高压差示扫描量热法(PDSC),热重-微商热重法(TG-DTG),研究了双基粘合剂(NC/NG体系)与二硝酰胺铵(ADN)之间的相互作用.结果表明,(NC/NG)/ADN体系中NG的分解峰由NC/NG中的207.0 ℃提前至159.8 ℃,在高压下该分解峰温提前至153.6 ℃.NC/ADN的分解峰温比NG/ADN提前了4.8 ℃,而且大部分的ADN没有被NG加速而提前分解,表明NC对ADN的作用比NG更强烈.此外,还用真空安定性试验(VST)评价了NC/NG与ADN的相容性,混合体系的净增放气量大于11 mL,属于严重不相容,证明在90 ℃下NC/NG与ADN之间也存在强烈的相互作用.

  11. Radical prostatectomy outcome when performed with PSA above 20 ng/ml.

    LENUS (Irish Health Repository)

    Connolly, S S

    2012-02-01

    Many centres currently do not offer radical prostatectomy (RP) to men with high-risk localised prostate cancer due to concerns regarding poor outcome, despite evidence to the contrary. We identified 18 men undergoing RP with serum PSA >20 ng\\/ml (high-risk by National Comprehensive Cancer Network definition) and minimum follow-up of 12 years (mean 13.5). Mean preoperative PSA was 37.0 ng\\/ml (Range 21.1-94.0). Prostatectomy pathology reported extracapsular disease in 16 (88.9%), positive surgical margins in 15 (83%) and positive pelvic lymph nodes in 5 (27.8%). Overall and cancer-specific survival at 5 and 10-years was 83.3%, 88.2%, 72% and 76.5% respectively. With complete follow-up 11 (61.1%) are alive, and 5 (27.8%) avoided any adjuvant therapy. Complete continence (defined as no involuntary urine leakage and no use of pads) was achieved in 60%, with partial continence in the remainder. We conclude that surgery for this aggressive variant of localised prostate cancer can result in satisfactory outcome.

  12. Phenomenological model for stress corrosion cracking of Types 316NG and 316 stainless steel

    International Nuclear Information System (INIS)

    A phenomenological model for both intergranular and transgranular stress corrosion cracking (SCC) in constant extension rate (CERT) tests is discussed. The model is based on an estimate of crack-tip strain rate obtained by use of a J-integral approach, a slip dissolution model for SCC, and a J-integral fracture criterion. The model is used to derive correlations between the SCC parameters (such as time to failure and average crack growth rate) and applied (nominal) strain rate. The model is extended to determine similar relationships between the SCC parameters and average near-crack-tip strain rate. The model predictions are compared with CERT test results obtained for Type 316NG stainless steel (SS) in water containing dissolved oxygen and impurities at 2890C over a range of strain rates between 10-5 and 10-7/s. Good agreement is obtained between the theoretical description and experimental results. The SCC behavior of Type 316NG SS is also compared with that of Type 316 SS to determine the relative SCC susceptibility of the two materials

  13. Aurora candidates from the chronicle of Qíng dynasty in several degrees of relevance

    Science.gov (United States)

    Kawamura, Akito D.; Hayakawa, Hisashi; Tamazawa, Harufumi; Miyahara, Hiroko; Isobe, Hiroaki

    2016-08-01

    We present the result of a survey of sunspots and auroras in Qíngshǐgǎo (清史稿), a draft chronicle of Qíng dynasty, for the period of 1559-1912 CE. This is a sequel to a series of works surveying historical sunspot and aurora records, and providing online data to the scientific community regarding the attained results. In total of this Qíngshǐgǎo survey, we found 111 records of night-sky luminous events with such keywords as vapor (氣, qì), cloud (雲, yún), and light (光, guāng), which may indicate auroras as well as some other phenomena. Similarly, a keyword survey for sunspots was conducted, but no sunspot record was found. In comparison with the aurora records in the western world, we found that 14 of the 111 records have a corresponding record of simultaneous observation in the western world, and hence are very likely to be aurora. In order to investigate the likeliness of the remainder of the record being aurora, we calculated the lunar age and the phase of a solar cycle for each record. After these calculations, a notable fraction of these records clustered near the full moon were to be found statistically doubtful in considerations with atmospheric optics; meanwhile, a few records of observations near the new moon could be more likely interpreted as being auroras, including three records during the Maunder minimum.

  14. Aurora Candidates from the Chronicle of Q\\={i}ng Dynasty in Several Degrees of Relevance

    CERN Document Server

    Kawamura, Akito D; Tamazawa, Harufumi; Miyahara, Hiroko; Isobe, Hiroaki

    2016-01-01

    We present the result of the survey of sunspots and auroras in ${\\it Q\\bar{\\imath}ngsh\\check{\\imath}ga\\check{o}}$, the draft chronicle of ${\\it Q\\bar{\\imath}ng}$ dynasty, for the period of 1559-1912 CE, as a sequel of the series of works surveying historical sunspot and aurora records, and providing online data to the scientific community regarding the attained results. In total of this ${\\it Q\\bar{\\imath}ngsh\\check{\\imath}ga\\check{o}}$ survey, we found 111 records of night-sky luminous events with the keywords such as vapor (${\\it q\\grave{\\imath}}$), cloud (${\\it y\\acute{u}n}$), and light (${\\it gu\\bar{a}ng}$), which may indicate auroras as well as some other phenomena. Similarly keyword survey for sunspots were done, but no sunspot record was found. In comparison with the aurora records in the western world, we found 14 of the 111 records have a corresponding record of simultaneous observation in the western world and hence are very likely to be aurora. In order to investigate the likeliness of the rest of ...

  15. Defining the appropriate radiation dose for pretreatment PSA no. <=no. 10 ng/mL prostate cancer

    International Nuclear Information System (INIS)

    Purpose: To investigate whether a dose response exists for biochemical no evidence of disease (bNED) control in prostate cancer patients with pretreatment prostate-specific antigen (PSA) no. =no. 7600 cGy. Median dose in these three groups was 7067 cGy, 7278 cGy, and 7734 cGy, respectively. Univariate analysis was performed to determine differences in bNED control (American Society for Therapeutic Radiology and Oncology [ASTRO] Consensus Guidelines definition of failure) by dose group for the entire cohort, for 310 good prognosis patients (T1-T2A, Gleason score 2-6, absence of PNI), and for 178 poor prognosis patients (T2B-T3 or Gleason score 7-10 or presence of PNI) . Multivariate analysis (MVA) was performed to determine if dose was an independent predictor of bNED control. Median follow-up was 36 months. Results: A dose response was not demonstrated for the entire group of patients with pretreatment PSA no. =no. 7600 cGy were associated with 5-year bNED control rates of 73%, 86%, and 89%, respectively (p = 0.12). MVA demonstrated prognosis group (p = 0.038) to be the only independent predictor of bNED control. Good prognosis patients had a 5-year bNED of 85% and no dose response was seen. The subgroup of poor prognosis patients demonstrated a 5-year bNED control rate of 81% and a dose response was seen for those receiving no. >=no. 7600 cGy, compared to the two lower dose groups (94% vs. 75% vs. 70%; p = 0.0062). MVA for the poor prognosis subset demonstrated dose (p = 0.01) to be the only independent predictor for improved bNED control. Conclusions: The poor prognosis subset of PSA no. <=no. 10 ng/mL prostate cancer patients benefit from dose escalation. A dose response is not demonstrated for prostate cancer patients with pretreatment PSA no. <=no. 10 ng/mL and other favorable features

  16. Freed by trust, to believe together: Pursuing global ecumenism with Küng and Tracy

    Directory of Open Access Journals (Sweden)

    Jakub Urbaniak

    2014-04-01

    Full Text Available Focus and scope Publication ethics Journal metrics Frequent asked questions Supporting agencies Contact us Reading tools Print this article Indexing metadata Review policy Email this article Email the author Post a Comment Translate content Powered by Translate Related items Show all ✔ Open access ✔ Global visibility ✔ Rapid publication ✔ Quality peer review ✔ Author retains copyright ✔ Personalised service ✔ Submit online Home About Author Reviewer Reader Support Archives Search Home > Vol 70, No 1 (2014 > Urbaniak Original Research Bookmark and Share Freed by trust, to believe together: Pursuing global ecumenism with Küng and Tracy Jakub Urbaniak HTS Teologiese Studies / Theological Studies; Vol 70, No 1 (2014, 9 pages. doi: 10.4102/hts.v70i1.2047 Submitted: 16 August 2013 Published: 11 April 2014 Abstract In the past decades it has emerged more clearly than before that Christian religion, which has so often contributed to human oppression, has rich theological resources that can be used to restore and perfect human freedom. These resources have been reflected upon not only by liberation theologians, but also within the ecumenically oriented theology of religions which targets what Hans Küng calls global responsibility based on global ethics. World religions have an essential role to play in rendering that global humanity more humane and free. The only way to accomplish this task leads through ongoing dialogue, directed both ad intra and ad extra, in the pursuit of a ‘global ecumenism’ which the present suggests and the future demands. For those liberating and unitive resources inherent in religious theory and praxis to be activated, fundamental trust in the reality of the world and of one’s own self appears indispensable. By deepening the theological insights of Hans Küng and David Tracy, the article seeks to explore the mutual correlation between such fundamental trust in reality and religious faith in God, interpreted

  17. Focused groundwater discharge of phosphorus to a eutrophic seepage lake (Lake Væng, Denmark)

    DEFF Research Database (Denmark)

    Kidmose, Jacob Baarstrøm; Nilsson, Bertel; Engesgaard, Peter Knudegaard;

    2013-01-01

    A study on Lake Væng in Denmark demonstrates a high potential for loading of phosphorous via groundwater to seepage lakes. Groundwater discharges are displayed as an important source of phosphorous to a lake due to: (1) high concentrations in the aquifer just below the lake, and (2) the main flow...... paths through the aquifer–lakebed interface either being overland flow through a seepage face, or focused in zones with very high discharge rates. In-lake springs have measured discharge of up to 7.45 m3 per m2 of lakebed per day. These findings were based on seepage meter measurements at 18 locations......, stable isotope (δ18O) analyses, temperature profiles and mapping of ice cover distribution. Groundwater–lake interaction was modelled with a 2D conceptual flow model (MODFLOW) with hydrogeology interpreted from catchment multi electrode profiling, on-lake ground-penetrating radar, well logging and...

  18. Nitric Oxide Synthase Inhibition by NG-Nitro-l-Arginine Methyl Ester Inhibits Tumor-Induced Angiogenesis in Mammary Tumors

    OpenAIRE

    Jadeski, Lorraine C.; Lala, Peeyush K.

    1999-01-01

    Using a murine breast cancer model, we earlier found a positive correlation between the expression of nitric oxide synthase (NOS) and tumor progression; treatment with inhibitors of NOS, NG-methyl-l-arginine (NMMA) and NG-nitro-l-arginine methyl ester (L-NAME), had antitumor and antimetastatic effects that were partly attributed to reduced tumor cell invasiveness. In the present study, we used a novel in vivo model of tumor angiogenesis using subcutaneous implants of tumor cells suspended in ...

  19. 40 Gb/s Lane Rate NG-PON using Electrical/Optical Duobinary, PAM-4 and Low Complex Equalizations

    DEFF Research Database (Denmark)

    Wei, J. L.; Grobe, Klaus; Wagner, Christoph;

    2016-01-01

    We present the first numerical investigation and comparison of 40-Gb/s lane rate electrical Duobinary, optical Duobinary and PAM-4 for NG-PONs incorporating low complex linear and nonlinear post-equalizations.......We present the first numerical investigation and comparison of 40-Gb/s lane rate electrical Duobinary, optical Duobinary and PAM-4 for NG-PONs incorporating low complex linear and nonlinear post-equalizations....

  20. Pagdalumat Sa Mga Sinaunang Di- Materyal Na Kultura Ng Mga B’laan Sa Brgy. Pisan, Kabacan, Cotabato Mula Sa Kanilang Kwentong Bayan

    Directory of Open Access Journals (Sweden)

    Dr. Nelia O. Du

    2014-12-01

    Full Text Available Nilayon ng pag-aaral na lumikom ng kwentong bayan ng mga B’laan sa Brgy. Pisan, Kabacan, North Cotabato at dalumatin ang kanilang di-materyal na kulturang masasalamin dito.Sa pagbibigay katuparan sa layunin, nasagot ang mga sumusunod na katanungan: (1 Ano ang mga kwentong bayan ng mga B’laan sa Pisan, Kabacan, Cotabato? (2 Anong mga di-materyal na kultura ang maaaring pagkakakilanlan ng mga B’laan ang masasalamin sa kanilang mga kwentong bayan? Paano ipinakita ang mga ito sa kanilang mga kwentong bayan? Saklaw ng pag-aaral na ito ang mga kwentong bayan na inuri ayon sa alamat, mito, salaysayin at pabula na kasasalaminan ng di-materyal na kultura ng mga B’laan na nakatira sa Brgy. Pisan, Kabacan, Cotabato. Kwantitatibo at Kwalitatibong disenyo ng pananaliksik ang ginamit sa pag-aaral at sinuri ang mga datos sa pamamagitan ng deskriptibong pamaraan partikular ang kontent analisis. Pamaraang indihenus o pangkatutubo ang pamaraang ginamit. Natuklasan na (1 May iba’t ibang kwentong bayan sa genre na alamat na etiolohikal, mito, salaysayin, at pabula ang mga Blaan; (2 Iba’t ibang di-materyal na kultura ang masasalamin sa mga kwentong bayan ng B’laan. Ito ang nabuong konklusyon: (1 Ang mga kwentong bayan ng B’laan ay kasasalaminan ng kanilang di-materyal na kultura.

  1. Nitrofurazone quantification in milk at the European Union minimum required performance limit of 1 ng g(-1): circumventing the semicarbazide problem.

    Science.gov (United States)

    Pearson, Ross A; Evans, Cameron; Bendall, Justin G

    2016-08-01

    Nitrofurazone is an antibiotic with carcinogenic properties. Efforts by regulatory authorities to control nitrofurazone from agricultural foods are an important public health measure that have, to some extent, been undermined by widespread use amongst laboratories of the unreliable marker metabolite semicarbazide. This work confirms what has long been suspected, namely that powdered dairy products that are initially free of semicarbazide develop semicarbazide under storage conditions such as occur normally across commercial supply chains. The low ng g(-)(1) levels of semicarbazide formed in this way are insufficient to present any food safety hazard. That such development of a marker metabolite is demonstrated to occur by innocent means effectively invalidates the use of semicarbazide as a marker metabolite for powdered dairy products, and exacerbates the regulatory need for a more suitable analytical methodology. In milk, unlike meat, nitrofurazone is known to remain stable and thus available for analysis in the intact form, rather than necessitating any use of a metabolite or fragment. However, no previous methodology that was capable of achieving the stringent European minimum required performance limit of 1 ng g(-)(1) when using intact nitrofurazone had been described for milk. This work describes a specific methodology using LC-MS/MS for milk and milk powder; it achieves detection of intact nitrofurazone (as well as furazolidone, furaltadone and nitrofurantoin) to levels well below 1 ng g(-)(1). Laboratories will no longer need to use semicarbazide as an unreliable marker metabolite for the analysis of nitrofurazone in dairy products, paving the way for regulatory authorities to better control nitrofurazone abuse with greater confidence. PMID:27455411

  2. Impact of pathological tumor stage for salvage radiotherapy after radical prostatectomy in patients with prostate-specific antigen < 1.0 ng/ml

    International Nuclear Information System (INIS)

    To evaluate prognostic factors in salvage radiotherapy (RT) for patients with pre-RT prostate-specific antigen (PSA) < 1.0 ng/ml. Between January 2000 and December 2009, 102 patients underwent salvage RT for biochemical failure after radical prostatectomy (RP). Re-failure of PSA after salvage RT was defined as a serum PSA value of 0.2 ng/ml or more above the postradiotherapy nadir followed by another higher value, a continued rise in serum PSA despite salvage RT, or initiation of systemic therapy after completion of salvage RT. Biochemical relapse-free survival (bRFS) was estimated using the Kaplan-Meier method. Multivariate analysis was performed using the Cox proportional hazards regression model. The median follow-up period was 44 months (range, 11-103 months). Forty-three patients experienced PSA re-failure after salvage RT. The 4-year bRFS was 50.9% (95% confidence interval [95% CI]: 39.4-62.5%). In the log-rank test, pT3-4 (p < 0.001) and preoperative PSA (p = 0.037) were selected as significant factors. In multivariate analysis, only pT3-4 was a prognostic factor (hazard ratio: 3.512 [95% CI: 1.535-8.037], p = 0.001). The 4-year bRFS rates for pT1-2 and pT3-4 were 79.2% (95% CI: 66.0-92.3%) and 31.7% (95% CI: 17.0-46.4%), respectively. In patients who have received salvage RT after RP with PSA < 1.0 ng/ml, pT stage and preoperative PSA were prognostic factors of bRFS. In particular, pT3-4 had a high risk for biochemical recurrence after salvage RT

  3. The GOLF-NG prototype and the solar European perspective for cosmic vision 2015-2025

    International Nuclear Information System (INIS)

    The progress on the dynamics of the radiative zone by global Doppler velocity measurements aboard SoHO (GOLF+ MDI) and with ground networks (BiSON and GONG) opens a new perspective for solar and stellar physics. It is why we prepare a new generation of solar resonant spectrometer. The objectives of the GOLF-NG instrument and its present status are described. We have demonstrated this year that most of the technical challenges have been successfully faced and the next steps are mentioned. We then recall the scientific questions that might be solved with the next generation of instruments in construction in different european laboratories to reach a complete 3D vision of our star from the core to the corona. Two formation flying missions DynaMICCS and HIRISE have been proposed to ESA in the framework of the 2015-2025 Cosmic Vision perspective to contribute to solve these questions. A strategy of measurements must be found for the next decade.

  4. Snow avalanche history in Parâng Mountains (Southern Carpathians revealed by dendrogeomorphic analyses

    Directory of Open Access Journals (Sweden)

    Flaviu MESEȘAN

    2014-11-01

    Full Text Available Parâng Mountains belong to Southern Carpathians, having the maximum altitude in Parângul Mare Peak (2519 m a.s.l.. The geology is dominated by crystalline schists, due to whom the relief is characterized by steep slopes. Between 1400 – 1700 m the average annual temperature is around 2-3ᴼC and the total precipitation about 1000-1200 mm/year. Coniferous belt extends roughly at the mentioned altitudes, being dominated by Norway spruce (Picea abies forests. Above 1800 m the average annual temperature drops below 2ᴼC and the average rainfalls reach 1200 mm/year, allowing the development of alpine shrubs and alpine grassland (Oancea et al., 1987. Crests and steep slopes in these subalpine areas are prone to snow accumulation and avalanche release. Despite the favorable conditions for avalanche trigger in this area, only one avalanche event is historically recorded, with severe impact on forest areas, but no destructive impact on infrastructure or fatalities.

  5. Determination of eight nitrosamines in water at the ng L{sup -1} levels by liquid chromatography coupled to atmospheric pressure chemical ionization tandem mass spectrometry

    Energy Technology Data Exchange (ETDEWEB)

    Ripolles, Cristina; Pitarch, Elena; Sancho, Juan V.; Lopez, Francisco J. [Research Institute for Pesticides and Water, University Jaume I, Avda. Sos Baynat, E-12071 Castellon (Spain); Hernandez, Felix, E-mail: felix.hernandez@qfa.uji.es [Research Institute for Pesticides and Water, University Jaume I, Avda. Sos Baynat, E-12071 Castellon (Spain)

    2011-09-19

    Highlights: {center_dot} Eight N-nitrosamines in water by LC(APCI)MS/MS QqQ analysis. {center_dot} Validation at two levels: 10 ng L{sup -1} (LOQ) and 100 ng L{sup -1} in drinking water. {center_dot} Developed method applied to different types of water samples. {center_dot} NDMA was the analyte more frequently detected and at the highest concentration levels. - Abstract: In this work, we have developed a sensitive method for detection and quantification of eight N-nitrosamines, N-nitrosodimethylamine (NDMA), N-nitrosomorpholine (NMor), N-nitrosomethylethylamine (NMEA), N-nitrosopirrolidine (NPyr), N-nitrosodiethylamine (NDEA), N-nitrosopiperidine (NPip), N-nitroso-n-dipropylamine (NDPA) and N-nitrosodi-n-butylamine (NDBA) in drinking water. The method is based on liquid chromatography coupled to tandem mass spectrometry, using atmospheric pressure chemical ionization (APCI) in positive mode with a triple quadrupole analyzer (QqQ). The simultaneous acquisition of two MS/MS transitions in selected reaction monitoring mode (SRM) for each compound, together with the evaluation of their relative intensity, allowed the simultaneous quantification and reliable identification in water at ppt levels. Empirical formula of the product ions selected was confirmed by UHPLC-(Q)TOF MS accurate mass measurements from reference standards. Prior to LC-MS/MS QqQ analysis, a preconcentration step by off-line SPE using coconut charcoal EPA 521 cartridges (by passing 500 mL of water sample) was necessary to improve the sensitivity and to meet regulation requirements. For accurate quantification, two isotope labelled nitrosamines (NDMA-d{sub 6} and NDPA-d{sub 14}) were added as surrogate internal standards to the samples. The optimized method was validated at two concentration levels (10 and 100 ng L{sup -1}) in drinking water samples, obtaining satisfactory recoveries (between 90 and 120%) and precision (RSD < 20%). Limits of detection were found to be in the range of 1-8 ng L{sup -1

  6. A Systematic Review of the Mysterious Caterpillar Fungus Ophiocordyceps sinensis in Dong-ChongXiaCao (冬蟲夏草 Dōng Chóng Xià Cǎo) and Related Bioactive Ingredients

    OpenAIRE

    Lo, Hui-Chen; Hsieh, Chienyan; Lin, Fang-Yi; Hsu, Tai-Hao

    2013-01-01

    The caterpillar fungus Ophiocordyceps sinensis (syn.† Cordyceps sinensis), which was originally used in traditional Tibetan and Chinese medicine, is called either “yartsa gunbu” or “DongChongXiaCao (冬蟲夏草 Dōng Chóng Xià Cǎo)” (“winter worm-summer grass”), respectively. The extremely high price of DongChongXiaCao, approximately USD $20,000 to 40,000 per kg, has led to it being regarded as “soft gold” in China. The multi-fungi hypothesis has been proposed for DongChongXiaCao; however, Hirsutella...

  7. The crossover from classical to quantum behavior in Duffing oscillator based on quantum state diffusion

    Indian Academy of Sciences (India)

    Y Ota; I Ohba

    2002-08-01

    The classical Duffing oscillator is a dissipative chaotic system, and so there is not a definite Hamiltonian. We quantize the Duffing oscillator on the basis of quantum state diffusion (QSD) which is a formulation for open quantum systems and a useful tool for analyzing nonlinear problems and classical limits. We can define a ‘Lyapunov exponent’, which corresponds to the classical one in the proper limit, and investigate the behavior of the system by varying the Planck constant effectively. We show that there exists a critical stage, where the behavior of the system crosses over from classical to quantum one.

  8. Effect of strength mismatch on fracture mechanical behavior of NG-DMW

    International Nuclear Information System (INIS)

    In modern pressurized water reactor (PWR) designs, dissimilar metal joints, e.g. reactor pressure vessel (RPV) safe-ends, are manufactured using a new weld design which takes advantage of narrow-gap (NG) welding technique. In addition to the new weld design, the filler metals have been changed from Alloys 82 and 182 to higher Cr containing Alloys 52 and 152 to ensure the structural integrity of the welds. In dissimilar metal welds (DMW), the mismatch in material properties between the two joined materials and their narrow local variation in different zones of the weld are of importance because the local strength mismatch state plays an important role in the fracture behavior of the weld. For the experimental determination of the local strength variations in a narrow-gap dissimilar metal weld (NG-DMW), a weld mock-up was manufactured using narrow-gap gas-tungsten arc welding (GTAW) method. The weld consisted of SA 508 pressure vessel steel with AISI 309L/308L cladding, AISI 304 piping steel, and Alloy 52 weld metal. The weld was characterized in two different heat treatment conditions, in as-welded condition and in post-weld heat treated (PWHT) condition. The microstructure of the weld mock-up was characterized using FEGSEM. The fusion zone (FZ) between the SA 508 pressure vessel steel and Alloy 52 weld metal was characterized using micro- and nano-hardness testing and the strength mismatch state of the FZ was determined with tensile testing using miniature-sized tensile testing specimens allowing the determination of the local tensile properties of the narrow weld zones near the fusion line (FL). The fracture mechanical testing was performed at room temperature to examine the effect of local strength mismatch on the fracture behavior and crack propagation. The results of the tensile tests revealed that Alloy 52 weld metal had close to equivalent strength with SA 508 base material and the highest strength mismatch existed between the SA 508 heat-affected zone (HAZ

  9. Pagdalumat Sa Mga Sinaunang Di- Materyal Na Kultura Ng Mga B’laan Sa Brgy. Pisan, Kabacan, Cotabato Mula Sa Kanilang Kwentong Bayan

    OpenAIRE

    Dr. Nelia O. Du

    2014-01-01

    Nilayon ng pag-aaral na lumikom ng kwentong bayan ng mga B’laan sa Brgy. Pisan, Kabacan, North Cotabato at dalumatin ang kanilang di-materyal na kulturang masasalamin dito.Sa pagbibigay katuparan sa layunin, nasagot ang mga sumusunod na katanungan: (1) Ano ang mga kwentong bayan ng mga B’laan sa Pisan, Kabacan, Cotabato? (2) Anong mga di-materyal na kultura ang maaaring pagkakakilanlan ng mga B’laan ang masasalamin sa kanilang mga kwentong bayan? Paano ipinakita ang mga ito sa ...

  10. Vapor generation and atom traps: Atomic absorption spectrometry at the ng/L level

    International Nuclear Information System (INIS)

    Atom-trapping atomic absorption spectrometry is a technique that allows detection at the ng/L level for several analytes such as As, Se, Sb, Pb, Bi, Cd, In, Tl, Te, Sn and Hg. The principle involves generation of volatile species, usually hydrides, trapping these species on the surface of an atom trap held at an optimized temperature and, finally, revolatilizing the analyte species by rapid heating of the trap and transporting them in a carrier gas to a heated quartz tube, as commonly used with hydride generation AAS systems. A transient signal having, in most cases, a full width at half maximum of less than 1 s is obtained. The atom trap may be a quartz surface or a W-coil; the former is heated externally and the latter is heated resistively. Both collection and revolatilization temperatures are optimized. In some cases, the W-coil itself is used as an electrothermal atomizer and a heated quartz tube is then not needed. The evolution of these traps starts with the well-known Watling's slotted quartz tube (SQT), continues with atom trapping SQT and finally reaches the present traps mentioned above. The analytical figures of merit for these traps need to be standardized. Naturally, enhancement is on characteristic concentration, C0, where the change in characteristic mass, m0, can be related to trapping efficiency. Novel terms are suggested for E, enhancement factor; such as Emax, maximum enhancement factor; Et, enhancement for 1.0 minute sampling and Ev, enhancement for 1.0 mL of sample. These figures will allow easy comparison of results from different laboratories as well as different analytes and/or traps

  11. Vapor generation and atom traps: Atomic absorption spectrometry at the ng/L level

    Energy Technology Data Exchange (ETDEWEB)

    Ataman, O. Yavuz [Department of Chemistry, Middle East Technical University, 06531 Ankara (Turkey)], E-mail: ataman@metu.edu.tr

    2008-08-15

    Atom-trapping atomic absorption spectrometry is a technique that allows detection at the ng/L level for several analytes such as As, Se, Sb, Pb, Bi, Cd, In, Tl, Te, Sn and Hg. The principle involves generation of volatile species, usually hydrides, trapping these species on the surface of an atom trap held at an optimized temperature and, finally, revolatilizing the analyte species by rapid heating of the trap and transporting them in a carrier gas to a heated quartz tube, as commonly used with hydride generation AAS systems. A transient signal having, in most cases, a full width at half maximum of less than 1 s is obtained. The atom trap may be a quartz surface or a W-coil; the former is heated externally and the latter is heated resistively. Both collection and revolatilization temperatures are optimized. In some cases, the W-coil itself is used as an electrothermal atomizer and a heated quartz tube is then not needed. The evolution of these traps starts with the well-known Watling's slotted quartz tube (SQT), continues with atom trapping SQT and finally reaches the present traps mentioned above. The analytical figures of merit for these traps need to be standardized. Naturally, enhancement is on characteristic concentration, C{sub 0}, where the change in characteristic mass, m{sub 0}, can be related to trapping efficiency. Novel terms are suggested for E, enhancement factor; such as E{sub max}, maximum enhancement factor; E{sub t}, enhancement for 1.0 minute sampling and E{sub v}, enhancement for 1.0 mL of sample. These figures will allow easy comparison of results from different laboratories as well as different analytes and/or traps.

  12. Vapor generation and atom traps: Atomic absorption spectrometry at the ng/L level

    Science.gov (United States)

    Ataman, O. Yavuz

    2008-08-01

    Atom-trapping atomic absorption spectrometry is a technique that allows detection at the ng/L level for several analytes such as As, Se, Sb, Pb, Bi, Cd, In, Tl, Te, Sn and Hg. The principle involves generation of volatile species, usually hydrides, trapping these species on the surface of an atom trap held at an optimized temperature and, finally, revolatilizing the analyte species by rapid heating of the trap and transporting them in a carrier gas to a heated quartz tube, as commonly used with hydride generation AAS systems. A transient signal having, in most cases, a full width at half maximum of less than 1 s is obtained. The atom trap may be a quartz surface or a W-coil; the former is heated externally and the latter is heated resistively. Both collection and revolatilization temperatures are optimized. In some cases, the W-coil itself is used as an electrothermal atomizer and a heated quartz tube is then not needed. The evolution of these traps starts with the well-known Watling's slotted quartz tube (SQT), continues with atom trapping SQT and finally reaches the present traps mentioned above. The analytical figures of merit for these traps need to be standardized. Naturally, enhancement is on characteristic concentration, C0, where the change in characteristic mass, m0, can be related to trapping efficiency. Novel terms are suggested for E, enhancement factor; such as Emax, maximum enhancement factor; Et, enhancement for 1.0 minute sampling and Ev, enhancement for 1.0 mL of sample. These figures will allow easy comparison of results from different laboratories as well as different analytes and/or traps.

  13. Potential of the multispectral synergism for observing ozone pollution combining measurements of IASI-NG and UVNS onboard EPS-SG

    Science.gov (United States)

    Costantino, Lorenzo; Cuesta, Juan; Emili, Emanuele; Foret, Gilles; Dufour, Gaëlle; Eremenko, Maxim; Chailleux, Yohann; Beekmann, Matthias; Flaud, Jean-Marie

    2016-04-01

    Current and future satellite observations offer a great potential for monitoring air quality on daily and global basis. However, measurements from currently in orbit sensors offer a limited capacity to probe surface concentrations of gaseous pollutants such as tropospheric ozone. Using single-band approaches based on IASI spaceborne thermal infrared measurements, only ozone down to the lower troposphere (3-4 km of altitude at lowest) may be observed (Eremenko et al., 2008). A recent multispectral method combining IASI and GOME-2 (both onboard MetOp satellites) spectra, respectively from the IR and UV, has shown enhanced sensitivity for probing ozone at the lowermost troposphere, but with maximum sensitivity around 2 km at lowest (Cuesta et al., 2013). Future spatial missions will be launched in the upcoming years, such as EPS-SG, carrying new generation sensors like IASI-NG and UVNS that will enhance the capacity to observe ozone pollution, and particularly when combining them through a multispectral synergism. This work presents an analysis of the potential of the multispectral synergism of IASI-NG and UVNS future spaceborne measurements for observing ozone pollution, performed in the framework of SURVEYOZON project (funded by the French Space Agency, CNES). For this, we develop a simulator of synthetic multispectral retrievals or pseudo-observations (referred as OSSE, Observing System Simulation Experiment) derived from IASI-NG+UVNS that will be compared to those from IASI+GOME2. In the first step of the OSSE, we create a pseudo-reality with simulations from the chemical-transport model MOCAGE (provided by CERFACS laboratory), where real O3 data from IASI and surface network stations have been assimilated for a realistic representation of ozone variability at the surface and the free troposphere. We focus on the high pollution event occurred in Europe on 10 July 2010. We use the coupled algorithms KOPRA+VLIDORT to simulate the spectra emitted, scattered and

  14. 游离NgR促进新生大鼠背根神经节突起体外生长%Role of soluble NgR in promotion of DRG neurite sprouting in vitro in rats

    Institute of Scientific and Technical Information of China (English)

    马永刚; 刘世清; 范里; 陶海鹰; 卫爱林

    2009-01-01

    目的 观察生物合成的游离NgR对新生大鼠原代背根神经节(DRG)神经元突起生长的影响.方法 以ViraPowerTM慢病毒将NgR(310)ecto基因转染人培养的骨髓基质细胞,收集培养上清获得游离NgR.分离新生大鼠DRG神经元并分为两组,对照组直接将神经元种植到经含中枢神经系统(CNS)髓鞘蛋白包被的培养皿中培养;实验组先将骨髓基质细胞培养上清加入含CNS髓鞘蛋白的培养皿中,孵育4 h后再植入DRG神经元.两组细胞均在培养24 h后固定,进行βⅢ-tubulin免疫组织化学染色,图像观察神经元及其突起生长情况.结果 基因修饰的骨髓基质细胞,转染后48 h间接免疫荧光的方法检测可见胞浆内荧光表达.种植24 h后对照组DRG细胞很少有突起长出;而实验组DRG细胞中60%的神经元长出突起,且突起较长.结论 游离NgR能够竞争性结合脊髓损伤后局部分泌的轴突生长抑制因子,从而保护生理性NgR,可能是促轴突再生和脊髓功能恢复的一种新策略.%Objective To observe the effect of soluble NgR on rat DRG neurite sprouting in vitro. Methods Gene encoding NgR(310)ecto was transduced into bone marrow stromal cells (BM-SCs). Soluble NgR was obtained from the supernatant of BMSCs culture. DRG neurons of neonate rat were isolated and allocated into two groups, ie, control group (DRG neurons were cultured in slides coa-ted with myelin protein of spinal cord) and experiment group (the slides were pre-incubated with the su-pernatant of BMSCs for four hours). BMSCs were immobilized after 24 hours of culture. Immunohis-tostaining with βⅢ-tubulin and fluorescence microscope were employed to analyze the neurite growth.Results Positive staining of intracytoplasm was found with indirect fluorescence microscope in the BM-SCs 48 hours following transduction. Little neurite was found sprout to DRG neurons in the control group 24 hours after transduction. In contrast, longer neurite was observed

  15. Multi-analyte approach for the determination of ng L{sup -1} levels of steroid hormones in unidentified aqueous samples

    Energy Technology Data Exchange (ETDEWEB)

    Noppe, H. [Ghent University, Faculty of Veterinary Medicine, Research group of Veterinary Public Health and Zoonoses, Laboratory of Chemical Analysis, Salisburylaan 133, B-9820 Merelbeke (Belgium)]. E-mail: Herlinde.Noppe@UGent.be; Verheyden, K. [Ghent University, Faculty of Veterinary Medicine, Research group of Veterinary Public Health and Zoonoses, Laboratory of Chemical Analysis, Salisburylaan 133, B-9820 Merelbeke (Belgium); Gillis, W. [Federal Agency for the Safety of the Food Chain, WTC III, Simon Bolivarlaan 30, B-1000 Brussels (Belgium); Courtheyn, D. [Federal Feed and Food Laboratory, Braemkasteelstraat 59, B-9050 Ghent (Belgium); Vanthemsche, P. [Federal Agency for the Safety of the Food Chain, WTC III, Simon Bolivarlaan 30, B-1000 Brussels (Belgium); De Brabander, H.F. [Ghent University, Faculty of Veterinary Medicine, Research group of Veterinary Public Health and Zoonoses, Laboratory of Chemical Analysis, Salisburylaan 133, B-9820 Merelbeke (Belgium)

    2007-03-14

    Since the 1970s, many analytical methods for the detection of illegal growth promoters, such as thyreostats, anabolics, {beta}-agonists and corticosteroids have been developed for a wide range of matrices of animal origin, including meat, fat, organ tissue, urine and faeces. The aim of this study was to develop an analytical method for the determination of ng L{sup -1} levels of estrogens, gestagens, androgens (EGAs) and corticosteroids in aqueous preparations (i.e. drinking water, drinking water supplements), commercially available on the 'black' market. For this, extraction was performed with Bakerbond C{sub 18} speedisk, a technique commonly used in environmental analysis. After fractionation, four fractions were collected using a methanol:water gradient program. Gas chromatography coupled to electron impact multiple mass spectrometry (GC-EI-MS{sup 2}) screening for the EGAs was carried out on the derivatized extracts. For the detection of corticosteroids, gas chromatography coupled to negative chemical ionization mass spectrometry (GC-NCI-MS) was used after oxidation of the extracts. Confirmation was done by liquid chromatography coupled to electrospray ionization multiple mass spectrometry (LC-ESI-MS{sup 2}). The combined use of GC and LC coupled to MS enabled the identification and quantification of anabolics and corticosteroids at the low ng L{sup -1} level. This study demonstrated the occurrence of both androgens and corticosteroids in different commercial aqueous samples.

  16. Draft Genome Sequence of Neisseria gonorrhoeae Strain NG_869 with Penicillin, Tetracycline and Ciprofloxacin Resistance Determinants Isolated from Malaysia.

    Science.gov (United States)

    Ang, Geik Yong; Yu, Choo Yee; Yong, Delicia Ann; Cheong, Yuet Meng; Yin, Wai-Fong; Chan, Kok-Gan

    2016-06-01

    Gonorrhea is a sexually transmitted infection caused by Neisseria gonorrhoeae and the increasing reports of multidrug-resistant gonococcal isolates are a global public health care concern. Herein, we report the genome sequence of N. gonorrhoeae strain NG_869 isolated from Malaysia which may provide insights into the drug resistance determinants in gonococcal bacteria. PMID:27570316

  17. Kui naine tahab olla kangelane : kestvus-perfomance "Äng - 161 km palja rindkerega" / Fideelia-Signe Roots

    Index Scriptorium Estoniae

    Roots, Fideelia-Signe, 1976-

    2014-01-01

    Fideelia-Signe Roots oma aktsioonist tegevuskunstiürituse "Äng" raames, mille käigus kunstnik kõndis jalgsi alasti ülakehaga 161 kilomeetrit Tartust Karepale eesmärgiga testida naise kehakuvandi piire. Kangelase ja superkangelase mõistest

  18. Potential of the future thermal infrared space-borne sensor IASI-NG to monitor lower tropospheric ozone

    Directory of Open Access Journals (Sweden)

    P. Sellitto

    2012-09-01

    Full Text Available The lower tropospheric (LT ozone concentration is a key factor for air quality (AQ. Observing efficiently LT ozone from space is crucial to monitor and better understand pollution phenomena occurring from inter-continental to local scales, and that have a proven noxious effect on the human health and the biosphere. The Infrared Atmospheric Sounder Interferometer (IASI flies on MetOp-A spacecraft and is planned to be launched in the next future as part of the other MetOp modules, i.e. MetOp-B and C. IASI has demonstrated to have the capability to single out the LT ozone signal only at favourable conditions, i.e. in presence of high thermal contrast scenarios. New generation satellite instruments are being designed to address several pressing geophysical issues, including a better observation capability of LT ozone. IASI-NG (New Generation, now having reached the accomplishment of design phase-A for launch in the 2020 timeframe as part of the EPS-SG (EUMETSAT Polar System-Second Generation, formerly post-EPS mission, may render feasible a better observation of AQ in terms of LT ozone. To evaluate the added-value brought by IASI-NG in this context, we developed a pseudo-observation simulator, including a direct simulator of thermal infrared spectra and a full inversion scheme to retrieve ozone concentration profiles. We produced one month (August 2009 of tropospheric ozone pseudo-observations based on both IASI and IASI-NG instrumental configurations. We compared the pseudo-observations and we found a clear improvement of LT ozone (up to 6 km altitude pseudo-observations quality for IASI-NG. The estimated total error is expected to be more than 35% smaller at 5 km, and 20% smaller for the LT ozone column. The total error on the LT ozone column is, on average, lower than 10% for IASI-NG. IASI-NG is expected to have a significantly better vertical sensitivity (monthly average degrees of freedom surface-6 km of 0.70 and to be sensitive at lower

  19. Potential of the future thermal infrared space-borne sensor IASI-NG to monitor lower tropospheric ozone

    Science.gov (United States)

    Sellitto, P.; Dufour, G.; Eremenko, M.; Cuesta, J.; Dauphin, P.; Forêt, G.; Gaubert, B.; Beekmann, M.; Peuch, V.-H.; Flaud, J.-M.

    2012-09-01

    The lower tropospheric (LT) ozone concentration is a key factor for air quality (AQ). Observing efficiently LT ozone from space is crucial to monitor and better understand pollution phenomena occurring from inter-continental to local scales, and that have a proven noxious effect on the human health and the biosphere. The Infrared Atmospheric Sounder Interferometer (IASI) flies on MetOp-A spacecraft and is planned to be launched in the next future as part of the other MetOp modules, i.e. MetOp-B and C. IASI has demonstrated to have the capability to single out the LT ozone signal only at favourable conditions, i.e. in presence of high thermal contrast scenarios. New generation satellite instruments are being designed to address several pressing geophysical issues, including a better observation capability of LT ozone. IASI-NG (New Generation), now having reached the accomplishment of design phase-A for launch in the 2020 timeframe as part of the EPS-SG (EUMETSAT Polar System-Second Generation, formerly post-EPS) mission, may render feasible a better observation of AQ in terms of LT ozone. To evaluate the added-value brought by IASI-NG in this context, we developed a pseudo-observation simulator, including a direct simulator of thermal infrared spectra and a full inversion scheme to retrieve ozone concentration profiles. We produced one month (August 2009) of tropospheric ozone pseudo-observations based on both IASI and IASI-NG instrumental configurations. We compared the pseudo-observations and we found a clear improvement of LT ozone (up to 6 km altitude) pseudo-observations quality for IASI-NG. The estimated total error is expected to be more than 35% smaller at 5 km, and 20% smaller for the LT ozone column. The total error on the LT ozone column is, on average, lower than 10% for IASI-NG. IASI-NG is expected to have a significantly better vertical sensitivity (monthly average degrees of freedom surface-6 km of 0.70) and to be sensitive at lower altitudes (more

  20. NG2, a common denominator for neuroinflammation, blood-brain barrier alteration, and oligodendrocyte precursor response in EAE, plays a role in dendritic cell activation.

    Science.gov (United States)

    Ferrara, Giovanni; Errede, Mariella; Girolamo, Francesco; Morando, Sara; Ivaldi, Federico; Panini, Nicolò; Bendotti, Caterina; Perris, Roberto; Furlan, Roberto; Virgintino, Daniela; Kerlero de Rosbo, Nicole; Uccelli, Antonio

    2016-07-01

    In adult CNS, nerve/glial-antigen 2 (NG2) is expressed by oligodendrocyte progenitor cells (OPCs) and is an early marker of pericyte activation in pathological conditions. NG2 could, therefore, play a role in experimental autoimmune encephalomyelitis (EAE), a disease associated with increased blood-brain barrier (BBB) permeability, inflammatory infiltrates, and CNS damage. We induced EAE in NG2 knock-out (NG2KO) mice and used laser confocal microscopy immunofluorescence and morphometry to dissect the effect of NG2 KO on CNS pathology. NG2KO mice developed milder EAE than their wild-type (WT) counterparts, with less intense neuropathology associated with a significant improvement in BBB stability. In contrast to WT mice, OPC numbers did not change in NG2KO mice during EAE. Through FACS and confocal microscopy, we found that NG2 was also expressed by immune cells, including T cells, macrophages, and dendritic cells (DCs). Assessment of recall T cell responses to the encephalitogen by proliferation assays and ELISA showed that, while WT and NG2KO T cells proliferated equally to the encephalitogenic peptide MOG35-55, NG2KO T cells were skewed towards a Th2-type response. Because DCs could be responsible for this effect, we assessed their expression of IL-12 by PCR and intracellular FACS. IL-12-expressing CD11c+ cells were significantly decreased in MOG35-55-primed NG2KO lymph node cells. Importantly, in WT mice, the proportion of IL-12-expressing cells was significantly lower in CD11c+ NG2- cells than in CD11c+ NG2+ cells. To assess the relevance of NG2 at immune system and CNS levels, we induced EAE in bone-marrow chimeric mice, generated with WT recipients of NG2KO bone-marrow cells and vice versa. Regardless of their original phenotype, mice receiving NG2KO bone marrow developed milder EAE than those receiving WT bone marrow. Our data suggest that NG2 plays a role in EAE not only at CNS/BBB level, but also at immune response level, impacting on DC activation and

  1. Contato, epidemias e corpo como agentes de transformação: um estudo sobre a AIDS entre os Índios Xokléng de Santa Catarina, Brasil Contact, epidemics, and the body as agents of change: a study of AIDS among the Xokléng indians in the State of Santa Catarina, Brazil

    Directory of Open Access Journals (Sweden)

    Flávio Braune Wiik

    2001-03-01

    Full Text Available A partir da análise dos episódios de AIDS que acometeram os Índios Xokléng em 1988, o presente artigo visa referir fenômenos de doença a rupturas e transformações socioculturais ocorridas nesse universo com base na história de contato com a sociedade abrangente. A interpretação e a análise da relação estabelecida são feitas à luz de teorias antropológicas acerca da centralidade que corpo, corporalidade e processos corporais degenerativos ocupam nas sociedades indígenas brasileiras. Propõe-se que corpo, sociedade e agentes macroconjunturais são articulados pela práxis; por isso, devem ser relacionados nos estudos socio-antropológicos concernentes aos fenômenos de doença. O artigo traz a descrição sucinta das epidemias advindas com o contato, tentando vinculá-las aos contextos históricos específicos que o marcaram. Categorias da etnomedicina, cosmologia e corporalidade Xokléng são associadas à organização social e aos casos de AIDS. Estes são apresentados tendo, como enfoque principal, a ligação estabelecida entre seu advento e as transformações no universo Xokléng em função da construção da Barragem Norte junto a sua Terra.Based on an analysis of AIDS cases among the Xokléng Indians in 1988, this article relates the illness phenomenon to socio-cultural disruptions and transformations in this indigenous group's universe, focusing on the history of their contact with Brazilian national society. The analysis and interpretation of this relationship are based on anthropological theories about the centrality of the body, corporeality, and degenerative bodily processes in Brazilian indigenous societies, according to which the body, society, and macro-situational elements are articulated by social praxis, and should thus be related in socio-anthropological studies of health-illness phenomena. The article briefly describes the history of epidemics emerging from contact and attempts to relate them to specific

  2. Along the Road: The Ngäbe-Buglé Struggle to Protect Environmental Resources in Panama

    Directory of Open Access Journals (Sweden)

    Rogelio Cansari

    2013-09-01

    Full Text Available Indigenous people in Panama do not enjoy full autonomy within their comarcas (traditional land reserves: they only control surface resources, while the state retains control of underground resources. This article analyses direct action by the Ngäbe-Buglé, who successfully defeated the latest attempt by the government to exploit underground resources within their comarcas. It describes government strategies for retaining control over Indigenous people’s land and analyses how the Ngäbe-Buglé counteracted these strategies with support from burgeoning civil society movements. We argue that this is due to an unprecedented alliance between Indigenous people and other social movements in Panama, as well as to the fact that Indigenous people have succeeded in federating all major civil society organizations around their discourses and actions.

  3. Effects of DHA on calcium influx in neuronal NG108-15 cells grown in serum-free medium

    Czech Academy of Sciences Publication Activity Database

    Málková, Barbora; Machová, Eva; Lisá, Věra; Doležal, Vladimír

    2005-01-01

    Roč. 94, č. S2 (2005), s. 169-169. ISSN 0022-3042. [Biennial Meeting of the International Society for Neurochemistry and the European Society for Neurochemistry /20./. 21.08.2005-26.08.2005, Innsbruck] R&D Projects: GA AV ČR(CZ) IAA5011206 Institutional research plan: CEZ:AV0Z50110509 Keywords : docosahexaenoic acid * cholinergic NG108-15 cells * defined growth medium * microfluorimetry * calcium imaging Subject RIV: ED - Physiology

  4. Voltage-gated sodium channel expression and action potential generation in differentiated NG108-15 cells

    OpenAIRE

    Liu Jinxu; Tu Huiyin; Zhang Dongze; Zheng Hong; Li Yu-Long

    2012-01-01

    Abstract Background The generation of action potential is required for stimulus-evoked neurotransmitter release in most neurons. Although various voltage-gated ion channels are involved in action potential production, the initiation of the action potential is mainly mediated by voltage-gated Na+ channels. In the present study, differentiation-induced changes of mRNA and protein expression of Na+ channels, Na+ currents, and cell membrane excitability were investigated in NG108-15 cells. Result...

  5. Performance of the Abbott RealTime CT/NG for Detection of Chlamydia trachomatis and Neisseria gonorrhoeae▿

    OpenAIRE

    Gaydos, C A; Cartwright, C P; Colaninno, P.; Welsch, J.; Holden, J; Ho, S. Y.; Webb, E. M.; Anderson, C.; Bertuzis, R.; Zhang, L.; Miller, T; Leckie, G.; Abravaya, K.; Robinson, J.

    2010-01-01

    A multicenter clinical study was conducted to evaluate the performance characteristics of the Abbott RealTime CT/NG assay, a multiplex real-time PCR assay, for simultaneous detection of Chlamydia trachomatis and Neisseria gonorrhoeae. The specimens were collected from a total of 3,832 male and female subjects at 16 geographically diverse sites. Specimens included male and female urine samples, male urethral swabs, female endocervical swabs, and self-collected and clinician-collected vaginal s...

  6. Functional expression of human D3 dopamine receptors in differentiated neuroblastoma x glioma NG108-15 cells.

    OpenAIRE

    Seabrook, G. R.; Kemp, J.A.; Freedman, S B; Patel, S.; Sinclair, H. A.; McAllister, G.

    1994-01-01

    This study describes the depression of calcium currents caused by activation of human D3 dopamine receptors which have been stably expressed in the neuroblastoma x glioma NG108-15 cell line. Transfected cells, which had been differentiated with prostaglandin E1 and isobutylmethylxanthine, exclusively expressed D3 receptor mRNA, which was demonstrated by reverse transcription polymerase chain reaction techniques. Transfected cells had high affinity binding sites for iodosulpiride, with a Kd of...

  7. Typing of Neisseria gonorrhoeae Opa and NG-MAST Gene of 12 Pairs of Sexual Contact Gonorrhea Patients in China

    Institute of Scientific and Technical Information of China (English)

    Hongxiang CHEN; Jiawen LI; Zhihong WU; Rongyi CHEN; Li XU; Ying YU; Jun SHUAI; Juan LI; Ming TAN; Yating TU

    2008-01-01

    To identify the genomic species of Neisseria gonorrhoeae, evaluate the difference between two molecular epidemiological methods and examine the relationship between sex partners and genotypes of bacteria, 24 strains of Neisseria gonorrhoeae isolated from the outpatients with gonorrhea were identified by using the Opa genotyping and NG-MAST genotyping and the relationship between genotypes and phenotypes was studied. Twenty-four strains of Neisseria gonorrhoeae fell into 10 ST genotypes by NG-MAST genotyping, whereas these strains were classified into 12 OT Opa genotypes by Opa genotyping. A new epidemic strain of ST genotype (217-86% homologisation 178) in China was identified. It is concluded that genotypes of each pair of strains from a pair of patient/sex partner besides 45/46 are the same, indicating that contagious infection take place between patient and the sex partner. Opa genotyping was more effective than NG-MAST genotyping in identifying the genomic species of Neisseria gonorrhoeae. ST genotype could be further classified into different Opa-types.

  8. Mesenchymal Tumors Can Derive from Ng2/Cspg4-Expressing Pericytes with β-Catenin Modulating the Neoplastic Phenotype

    Directory of Open Access Journals (Sweden)

    Shingo Sato

    2016-07-01

    Full Text Available The cell of origin for most mesenchymal tumors is unclear. One cell type that contributes to this lineages is the pericyte, a cell expressing Ng2/Cspg4. Using lineage tracing, we demonstrated that bone and soft tissue sarcomas driven by the deletion of the Trp53 tumor suppressor, or desmoid tumors driven by a mutation in Apc, can derive from cells expressing Ng2/Cspg4. Deletion of the Trp53 tumor suppressor gene in these cells resulted in the bone and soft tissue sarcomas that closely resemble human sarcomas, while stabilizing β-catenin in this same cell type caused desmoid tumors. Comparing expression between Ng2/Cspg4-expressing pericytes lacking Trp53 and sarcomas that arose from deletion of Trp53 showed inhibition of β-catenin signaling in the sarcomas. Activation of β-catenin inhibited the formation and growth of sarcomas. Thus, pericytes can be a cell of origin for mesenchymal tumors, and β-catenin dysregulation plays an important role in the neoplastic phenotype.

  9. Tungo sa Hardin ng Tao: “Si Bonifacio ang pinakauna, hindi lamang sa Pilipinas, kundi sa buong Indonesia, oo, sa buong Asia na nanggaling sa, at edukado bilang, proletaryado, na nag-organisa ng mga proletaryo.” ni Tan Malaka

    Directory of Open Access Journals (Sweden)

    Ramon Guillermo

    2013-06-01

    Full Text Available Salin ito ng sanaysay na “Ke Taman Manusia” (sinulat taong 1943 nakathang-isip ng Indones na lider komunista na si Tan Malaka o Ibrahimgelar Datuk Tan Malaka (1897-1949 hinggil sa isang “Taman Manusia”o “Hardin ng Tao” na itatayo ng darating na Republika ng Indonesia sahinaharap. Dahil sa futuristiko nitong perspektiba, maihahalintuladang obrang ito ni Tan Malaka sa sanaysay ni Jose Rizal na “Filipinasdentro de cien años” (Ang Pilipinas sa loob ng isang dantaon (1891.Galing ito sa napakalaking akdang pilosopikal ni Tan Malaka napinamagatang Madilog: Materialisme, Dialektika, Logika (1948(Larawan 1 at tila tumatayo bilang pansarang mga repleksyon nito(Magnis-Suseno, 2005, pp. 205-233. Ang akdang ito ay unanginilathala noong 1951 ng Terbitan Widjaya sa Jakarta.

  10. Assessment of erosion hazard with the USLE and GIS: A case study of the Upper Ewaso Ng'iro North basin of Kenya

    Science.gov (United States)

    Mati, Bancy M.; Morgan, Royston PC; Gichuki, Francis N.; Quinton, John N.; Brewer, Tim R.; Liniger, Hans P.

    The Universal Soil Loss Equation (USLE) was used to predict soil erosion hazard in the Upper Ewaso Ng'iro North basin of Kenya using data from erosion plots and reconnaissance surveys. The R-factor (rainfall erosivity) was determined by extrapolation from the rainfall amount-erosivity relationship, using data from autographic records. The K-factor (soil erodibility) was determined using data obtained by laboratory analysis of soil samples collected from 83 test sites in the basin. The LS-factors (slope length and steepness) were determined from a digital elevation model (DEM) of the basin, while the C-factor (crop and management) was determined from vegetation cover data, obtained from SPOT imagery and field surveys. The P-factor (conservation practice) was estimated from maps of soil conservation, obtained from the Ministry of Agriculture. A raster-based Geographic Information System (GIS) was used to interactively calculate soil loss and map erosion hazard. The results obtained were comparable to measured soil loss values from erosion plots. About 36 percent of the Upper Ewaso Ng'iro basin was predicted to have high risk of erosion, most of this land being in the overgrazed rangelands. Land use and management were the major factors associated with soil erosion, and — to a lesser extent — the topography. Due to the reconnaissance scale of this study there were limitations in determining reliable LS, C and P factors for the USLE. In addition, there is a need for research to determine appropriate P-factors for local soil conservation practices, such as trash lines, fanya-juu terraces and stone lines.

  11. Meditation In The Emotional Intelligence Improvement Among Russian-Speakıng Migrants In Germany

    OpenAIRE

    Sergey Afanasyev

    2014-01-01

    The analysis of meditation as a factor of an individual’s psychological and emotional well-being as well as the comparison of main emotional intelligence quantitative measurement methods and the justification of the method used in the study were performed. Main features of the migrants’ social-psychological adaptation were considered. The key role of interpersonal emotional intelligence competency in migrants’ social-psychological adaptation process was identified. The interrelation between m...

  12. Novel cost-effective quality control approach for the Cepheid Xpert CT/NG assay for the detection of Chlamydia Trachomatis and Neisseria Gonorrhoeae.

    Science.gov (United States)

    Chan, Maurice; Jiang, Boran; Ng, Siew Yong Lily; Tan, Thean Yen

    2016-06-01

    The Xpert CT/NG is a rapid assay for detection of Neisseria gonorrhoeae and Chlamydia trachomatis. QC materials must be formulated to emulate human specimens, and are prohibitively expensive. A creative, cost-effective QC approach is proposed. The acceptable sample types for the Xpert CT/NG assay were extended to include eye swabs. PMID:27091503

  13. Photo-dynamics of the lyophilized photo-activated adenylate cyclase NgPAC2 from the amoeboflagellate Naegleria gruberi NEG-M strain

    Energy Technology Data Exchange (ETDEWEB)

    Penzkofer, A., E-mail: alfons.penzkofer@physik.uni-regensburg.de [Fakultät für Physik, Universität Regensburg, Universitätsstrasse 31, D-93053 Regensburg (Germany); Tanwar, M.; Veetil, S.K.; Kateriya, S. [Department of Biochemistry, University of Delhi South Campus, Benito Juarez Road, New Delhi 110021 (India); Stierl, M.; Hegemann, P. [Institut für Biologie/Experimentelle Biophysik, Humboldt Universität zu Berlin, Invalidenstrasse 42, D-10115 Berlin (Germany)

    2013-09-23

    Highlights: • Lyophilizing of NgPAC2 from Naegleria gruberi caused loss of BLUF domain activity. • Photo-induced tyrosine to flavin electron transfer in lyophilized NgPAC2. • Photo-induced Tyr–Tyr cross-linking to o,o′-dityrosine in lyophilized NgPAC2. • Photo-induced partial flavin cofactor reduction in lyophilized NgPAC2. • Two NgPAC2 conformations with fast and slow photo-induced electron transfer. - Abstract: The absorption and emission spectroscopic behavior of lyophilized photo-activated adenylate cyclase NgPAC2 from the amoeboflagellate Naegleria gruberi NEG-M strain consisting of a BLUF domain (BLUF = Blue Light sensor Using Flavin) and a cyclase homology domain was studied in the dark, during blue-light exposure and after blue-light exposure at a temperature of 4 °C. The BLUF domain photo-cycle dynamics observed for snap-frozen NgPAC2 was lost by lyophilization (no signaling state formation with flavin absorption red-shift). Instead, blue-light photo-excitation of lyophilized NgPAC2 caused sterically restricted Tyr–Tyr cross-linking (o,o′-ditysosine formation) and partial flavin cofactor reduction.

  14. Sammenhæng mellem fysisk aktivitet i barndom hhv. voksenliv samt holdning til fysisk aktivitet i pædagogisk arbejde

    DEFF Research Database (Denmark)

    Lybæk-Hansen, Esper

    Der er en tæt sammenhæng mellem den pædagogstuderendes fysiske aktivitet i barndommen og fysiske aktivitet i det voksne liv. Den sociale vane (habitus) spiller i denne sammenhæng en afgørende rolle for praksis i det voksne liv! Pædagogstuderende har generelt et positivt forhold til fysisk aktivit...

  15. Photo-dynamics of the lyophilized photo-activated adenylate cyclase NgPAC2 from the amoeboflagellate Naegleria gruberi NEG-M strain

    International Nuclear Information System (INIS)

    Highlights: • Lyophilizing of NgPAC2 from Naegleria gruberi caused loss of BLUF domain activity. • Photo-induced tyrosine to flavin electron transfer in lyophilized NgPAC2. • Photo-induced Tyr–Tyr cross-linking to o,o′-dityrosine in lyophilized NgPAC2. • Photo-induced partial flavin cofactor reduction in lyophilized NgPAC2. • Two NgPAC2 conformations with fast and slow photo-induced electron transfer. - Abstract: The absorption and emission spectroscopic behavior of lyophilized photo-activated adenylate cyclase NgPAC2 from the amoeboflagellate Naegleria gruberi NEG-M strain consisting of a BLUF domain (BLUF = Blue Light sensor Using Flavin) and a cyclase homology domain was studied in the dark, during blue-light exposure and after blue-light exposure at a temperature of 4 °C. The BLUF domain photo-cycle dynamics observed for snap-frozen NgPAC2 was lost by lyophilization (no signaling state formation with flavin absorption red-shift). Instead, blue-light photo-excitation of lyophilized NgPAC2 caused sterically restricted Tyr–Tyr cross-linking (o,o′-ditysosine formation) and partial flavin cofactor reduction

  16. Pagsusuri sa mga Balyung Nakapaloob sa mga Salawikain ng mga Tiruray Sa South Upi, Maguindanao, Philippines

    Directory of Open Access Journals (Sweden)

    Maria Luz D. Calibayan

    2015-12-01

    Full Text Available This study sought to collect, record, translate, and analyze the Tiruray proverbs. Specifically the study aimed to find out the following: (1 What are the existing proverbs of Tiruray in South Upi, Maguindanao? (2What are the values found as expressed in the proverbs of Tiruraythat have been successfully preserved from their ancestors? The scope of this study was confined to the collected proverbs of Tiruray in South Upi, Maguindanao. The gatheredproverbs were transcribed from Tiruray to Filipino languageand the analysis of values from the translated proverbs was in accordance with the ideas of Andres (1985 and Timbreza (2003. The research design adopted in the study is descriptive content analysis because the main objective of the study was to analyse the values found in Tiruray proverbs. Findings revealed that the Tiruray have rich in oral literary piecessuch as proverbs which are transmitted by word of mouth from generation to generation. The Tiruray proverbs contain differenthuman values that teach or remind people how to live godly lives. Further, Tiruray proverbs conveyed message on how they value peace and harmony in the community for they are peace-loving people. The study of Tiruray proverbs could increase the body of knowledge about the cultural traits of Tiruray who are unique people and have rich cultural heritage.

  17. Analysis Of The Performance Evaluation Scale For Strıng Instruments In Terms Of Validity And Reliability

    OpenAIRE

    ÇİFTCİ, Ersan; KURTULDU, M KAYHAN

    2010-01-01

    In this study, the performance evaluation scale developed for the string instrument classes was analysed in terms of validity and reliability. The scale, constructed with the help of the evaluation degrees designed by the researchers; was used in evaluating students in final examinations in two different departments by 6 instructors in total. Statistical measurements related to the resulting data were made and reliabilitiy of the scale was tested. In the statistical process, firstly alpha fac...

  18. Structure of Naegleria Tet-like dioxygenase (NgTet1) in complexes with a reaction intermediate 5-hydroxymethylcytosine DNA.

    Science.gov (United States)

    Hashimoto, Hideharu; Pais, June E; Dai, Nan; Corrêa, Ivan R; Zhang, Xing; Zheng, Yu; Cheng, Xiaodong

    2015-12-15

    The family of ten-eleven translocation (Tet) dioxygenases is widely distributed across the eukaryotic tree of life, from mammals to the amoeboflagellate Naegleria gruberi. Like mammalian Tet proteins, the Naegleria Tet-like protein, NgTet1, acts on 5-methylcytosine (5mC) and generates 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC) in three consecutive, Fe(II)- and α-ketoglutarate-dependent oxidation reactions. The two intermediates, 5hmC and 5fC, could be considered either as the reaction product of the previous enzymatic cycle or the substrate for the next cycle. Here we present a new crystal structure of NgTet1 in complex with DNA containing a 5hmC. Along with the previously solved NgTet1-5mC structure, the two complexes offer a detailed picture of the active site at individual stages of the reaction cycle. In the crystal, the hydroxymethyl (OH-CH2-) moiety of 5hmC points to the metal center, representing the reaction product of 5mC hydroxylation. The hydroxyl oxygen atom could be rotated away from the metal center, to a hydrophobic pocket formed by Ala212, Val293 and Phe295. Such rotation turns the hydroxyl oxygen atom away from the product conformation, and exposes the target CH2 towards the metal-ligand water molecule, where a dioxygen O2 molecule would occupy to initiate the next round of reaction by abstracting a hydrogen atom from the substrate. The Ala212-to-Val (A212V) mutant profoundly limits the product to 5hmC, probably because the reduced hydrophobic pocket size restricts the binding of 5hmC as a substrate. PMID:26323320

  19. Isolating the pre-main sequence in Collinder34, NGC3293, NGC3766 and NG6231

    CERN Document Server

    Saurin, T A; Bonatto, C

    2015-01-01

    We employed field star decontaminated 2MASS photometry to study four nearby optical embedded clusters -- Collinder34, NGC3293, NGC3766 and NGC6231 -- obtaining deep colour-magnitude diagrams and stellar radial density profiles. We found what seems to be pre-main sequences detached in different amounts from main sequences in these diagrams. The structural analysis of each cluster revealed different radial distributions for these two sequences. We argued that the detached evolutionary sequences in our sample may be evidence of sequential star formation. Finally, we compared the sample cluster parameters with those of other young clusters in the literature and point out evidence that NGC3766 and NGC6231 might be evolving to OB associations.

  20. Structure of Naegleria Tet-like dioxygenase (NgTet1) in complexes with a reaction intermediate 5-hydroxymethylcytosine DNA

    OpenAIRE

    Hashimoto, Hideharu; Pais, June E.; Dai, Nan; Corrêa, Ivan R; Zhang, Xing; Zheng, Yu; Cheng, Xiaodong

    2015-01-01

    The family of ten-eleven translocation (Tet) dioxygenases is widely distributed across the eukaryotic tree of life, from mammals to the amoeboflagellate Naegleria gruberi. Like mammalian Tet proteins, the Naegleria Tet-like protein, NgTet1, acts on 5-methylcytosine (5mC) and generates 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC) in three consecutive, Fe(II)- and α-ketoglutarate-dependent oxidation reactions. The two intermediates, 5hmC and 5fC, could be...

  1. Integratıon Wıthin the EU Accountıng Professıon

    OpenAIRE

    Sevki Celik

    2015-01-01

    Integratıon Wıthin the EU Accountıng Professıon Not to accept globalization in relation to the IFRS ‘good practice being in common practice from the beginning only by multi-national companies, means to stay outside of the advancements uccuring in the world. Our country has become more transparent capital markets, accounting standards for accounting, tax odaklılıktan get rid of IFRS must be appropriate. Accountant is the largest contributor to the increasing competitive environment, to emer...

  2. Silencing CTL1 mRNA decreases choline transport and cell growth in NG108-15 cells

    Czech Academy of Sciences Publication Activity Database

    Machová, Eva; Michal, Pavel; Lisá, Věra; Doležal, Vladimír

    Praha, 2005. s. 138-138. [Conference of the Czech Neuroscience Society /5./, The Annual Meeting of the Network of European Neuroscience Institutes. 19.11.2005-21.11.2005, Prague] R&D Projects: GA AV ČR(CZ) IAA5011206; GA MŠk(CZ) LC554 Grant ostatní: GA-(XE) QLK1-CT-2002-00172 Institutional research plan: CEZ:AV0Z50110509 Keywords : CTL1 protein knockdown * RNA interference * NG108-15 cells * cell growth * choline transport Subject RIV: ED - Physiology

  3. Strata of standardization: the Phong Nha dialect of Vietnamese (Quảng Bình Province) in historical perspective

    OpenAIRE

    Michaud, Alexis; Ferlus, Michel; Nguyễn, Minh-Châu

    2015-01-01

    The present research, based on first-hand data, is intended as a contribution to the study of the present-day diversity of lesser-described Vietnamese dialects, and of the range of evolutionary paths to which they testify. The Vietnamese dialect of the hamlet of Phong Nha (commune of Sơn Trạch, county of Bố Trạch, Quảng Bình) is one of the "heterodox" dialects of Vietnamese, which are known to present considerable interest for the historical study of Vietnamese and of the Vietic group at larg...

  4. Background identification and suppression for the measurement of (n,g) reactions with the DANCE array at LANSCE

    CERN Document Server

    Reifarth, R; Alpizar-Vicente, A; Browne, J C; Esch, E -I; Greife, U; Haight, R C; Hatarik, R; Kronenberg, A; O'Donnell, J M; Rundberg, R S; Ullmann, J L; Vieira, D J; Wilhelmy, J B; Wouters, J M

    2013-01-01

    In the commissioning phase of the DANCE project (Detector for Advanced Neutron Capture Experiments) measurements have been performed with special emphasis on the identification and suppression of possible backgrounds for the planned (n,g) experiments. This report describes several background sources, observed in the experiment or anticipated from simulations, which will need to be suppressed in this and in similar detectors that are planned at other facilities. First successes are documented in the suppression of background from scattered neutrons captured in the detector as well as from the internal radiation. Experimental results and simulations using the GEANT code are compared.

  5. TESTİNG TWIN DEFICITS HYPHESIS BY BOUND TESTING APPROACH: TURKEY CASE

    Directory of Open Access Journals (Sweden)

    Utku ALTUNÖZ

    2014-06-01

    Full Text Available Twin deficit identity is used to refer to a nation’s current account deficits and a simultaneous fiscal deficit. The term became widely used in the 1980s until the 1990s.Chronic external deficit problems have been encountered since the adoption of the outward-oriented growth model for Turkish economy. This study aim the validity of twin deficits hypothesis (the relationship between the budget deficit and the current account deficit by using the data period 1998:1-2012:3 in Turkey. The Public deficit indicators represented by budget deficit / GNP, reel exchange rate index and government domestic borrowing stock. In addition, current balance deficit is an another indicator for model. For this purpose, bound test approach developed by Pesaran et al.(2001 was applied by using data of determined period. According the result of econometric analysis, there is no long-run relationship between current balance deficit and budget deficit but there is a strong positive relationship between current balance deficit and budget deficit in the short-run.

  6. Prevalence and Risk Factors of Prostate Cancer in Chinese Men with PSA 4–10 ng/mL Who Underwent TRUS-Guided Prostate Biopsy: The Utilization of PAMD Score

    Science.gov (United States)

    Fang, Dong; Ren, Da; Zhao, Chenglin; Li, Xuesong; Yu, Wei; Wang, Rui; Wang, Huihui; Xi, Chenguang; He, Qun; Wang, Xiaoying; Xin, Zhongcheng; Zhou, Liqun

    2015-01-01

    Purpose. To elucidate the characteristics and risk factors for positive biopsy outcomes in Chinese patients with prostate specific antigen (PSA) 4–10 ng/mL and develop a risk-stratification score model. Methods. The data of 345 patients who underwent transrectal ultrasound-guided prostate biopsy between 2011 and 2013 was retrospectively analyzed. Digital rectal examination (DRE), prostate volume (PV), magnetic resonance imaging (MRI), and smoking status were also collected. Positive biopsy outcomes were defined as prostate cancer (PCa) and high grade PCa (HGPCa, Gleason Score ≥ 7). Results. The median PSA was 7.15 (IQR 5.91–8.45) ng/mL. Overall 138 patients (40.0%) were shown to have PCa, including 100 patients (29.0%) with HGPCa. Smaller PV, elder age, MRI results, and positive DRE were proved to be predictive factors for positive biopsy outcomes in both univariate and multivariate analysis. We developed a “PAMD” score which combined the four factors to categorize patients into three risk groups, and the model performed good predictive sensitivity and specificity. Conclusion. The prevalence of prostate cancer in Chinese patients with PSA 4–10 ng/mL was 40%, including 29% patients with high grade disease. DRE, age, MRI, and PV were predictive factors for positive biopsy outcomes, and the PAMD score model could be utilized for risk-stratification and decision-making. PMID:26557679

  7. Prevalence and Risk Factors of Prostate Cancer in Chinese Men with PSA 4–10 ng/mL Who Underwent TRUS-Guided Prostate Biopsy: The Utilization of PAMD Score

    Directory of Open Access Journals (Sweden)

    Dong Fang

    2015-01-01

    Full Text Available Purpose. To elucidate the characteristics and risk factors for positive biopsy outcomes in Chinese patients with prostate specific antigen (PSA 4–10 ng/mL and develop a risk-stratification score model. Methods. The data of 345 patients who underwent transrectal ultrasound-guided prostate biopsy between 2011 and 2013 was retrospectively analyzed. Digital rectal examination (DRE, prostate volume (PV, magnetic resonance imaging (MRI, and smoking status were also collected. Positive biopsy outcomes were defined as prostate cancer (PCa and high grade PCa (HGPCa, Gleason Score≥7. Results. The median PSA was 7.15 (IQR 5.91–8.45 ng/mL. Overall 138 patients (40.0% were shown to have PCa, including 100 patients (29.0% with HGPCa. Smaller PV, elder age, MRI results, and positive DRE were proved to be predictive factors for positive biopsy outcomes in both univariate and multivariate analysis. We developed a “PAMD” score which combined the four factors to categorize patients into three risk groups, and the model performed good predictive sensitivity and specificity. Conclusion. The prevalence of prostate cancer in Chinese patients with PSA 4–10 ng/mL was 40%, including 29% patients with high grade disease. DRE, age, MRI, and PV were predictive factors for positive biopsy outcomes, and the PAMD score model could be utilized for risk-stratification and decision-making.

  8. Can a Gleason 6 or Less Microfocus of Prostate Cancer in One Biopsy and Prostate-Specific Antigen Level <10 ng/mL Be Defined as the Archetype of Low-Risk Prostate Disease?

    Directory of Open Access Journals (Sweden)

    Gianluigi Taverna

    2012-01-01

    Full Text Available Prostate cancer (PC remains a cause of death worldwide. Here we investigate whether a single microfocus of PC at the biopsy (graded as Gleason 6 or less, ≤5% occupancy and the PSA <10 ng/mL can define the archetype of low-risk prostate disease. 4500 consecutive patients were enrolled. Among them, 134 patients with a single micro-focus of PC were followed up, and the parameters influencing the biochemical relapse (BR were analysed. Out of 134 patients, 94 had clinically significant disease, specifically in 74.26% of the patients with PSA <10 ng/mL. Positive surgical margins and the extracapsular invasion were found in 29.1% and 51.4% patients, respectively. BR was observed in 29.6% of the patients. Cox regression evidenced a correlation between the BR and Gleason grade at the retropubic radical prostatectomy (RRP, capsular invasion, and the presence of positive surgical margins. Multivariate regression analysis showed a statistically significant correlation between the presence of surgical margins at the RRP and BR. Considering a single micro-focus of PC at the biopsy and PSA serum level <10 ng/mL, clinically significant disease was found in 74.26% patients and only positive surgical margins are useful for predicting the BR.

  9. Sox2-Mediated Conversion of NG2 Glia into Induced Neurons in the Injured Adult Cerebral Cortex

    Directory of Open Access Journals (Sweden)

    Christophe Heinrich

    2014-12-01

    Full Text Available The adult cerebral cortex lacks the capacity to replace degenerated neurons following traumatic injury. Conversion of nonneuronal cells into induced neurons has been proposed as an innovative strategy toward brain repair. Here, we show that retrovirus-mediated expression of the transcription factors Sox2 and Ascl1, but strikingly also Sox2 alone, can induce the conversion of genetically fate-mapped NG2 glia into induced doublecortin (DCX+ neurons in the adult mouse cerebral cortex following stab wound injury in vivo. In contrast, lentiviral expression of Sox2 in the unlesioned cortex failed to convert oligodendroglial and astroglial cells into DCX+ cells. Neurons induced following injury mature morphologically and some acquire NeuN while losing DCX. Patch-clamp recording of slices containing Sox2- and/or Ascl1-transduced cells revealed that a substantial fraction of these cells receive synaptic inputs from neurons neighboring the injury site. Thus, NG2 glia represent a potential target for reprogramming strategies toward cortical repair.

  10. Chronic stress regulates NG2⁺ cell maturation and myelination in the prefrontal cortex through induction of death receptor 6.

    Science.gov (United States)

    Yang, Youjun; Zhang, Yini; Luo, Fei; Li, Baoming

    2016-03-01

    Chronic stress significantly affects neuron morphometry and function in the prefrontal cortex, a brain region controlling cognition and emotion. However, whether and how chronic stress regulates the maturation of NG2-expressing oligodendrocyte precursor cell (NG2(+) cell) and the importance of these changes remained unknown. Here, we report that exposing adult mice to chronic stress results in NG2(+) cell atrophy and myelination arrested in the medial prefrontal cortex (mPFC), and impaired mPFC-dependent functions. These alterations, are phenocopied by overexpression of death receptor 6 (DR6) in NG2(+) cell. Conversely, selectively silencing of DR6 in the NG2(+) cell can partly rescue NG2(+) cell atrophy and cognitive deficiency caused by chronic stress. We further demonstrate that myelination appears necessary for mPFC-dependent cognitive processes, as lysolecithin (LPC)-induced demyelination specifically in the mPFC is sufficient to cause these behavioral and cognitive impairments. Our results indicate that chronic stress impairs cognitive functions, at least in part, through modulation of NG2(+) cell maturation and myelination, and suggest that myelination is require for normal cognitive functions. PMID:26772637

  11. TEACHER TRAINERS VIEWS OF SERVİCE LEARNİNG COURSE

    Directory of Open Access Journals (Sweden)

    Serdar ARCAGÖK

    2013-06-01

    theoretical and 2 application class hour in a week (M.Ü., 2009. One of the main objectives of education is developing individuals according to social requirements and placing developed individuals to the society (Musolf, 2001: 286. Since teaching occupation is a profession which requires social responsibility, it is thought that CSA course will have reasonable attributions to the students. This study aims to detect the pre-service elementary teachers’ and trainers’ views about CSA course. According to this aim, following questions are tried to be answered; In this study, Community Service Applications in Primary Education Undergraduate Program was evaluated according to the views of pre-service teachers and instructors. İt was used mixed methods in this study. At the first stage of the study, one of the qualitative study methods, “Phenomenology Design” pattern was used to define instructors’ views about CSA in Primary Education Department. At the second stage, to define the pre-service teachers’ views about CSA, a scale was applied to them. The population of the study is composed of the instructors who work in 2010-2011 academic year spring semester in Çanakkale Onsekiz Mart University and the pre-service elemantary teachers who are studying at Çanakkale Onsekiz Mart University during the 2010-2011 academic year. In this study mixed methods was used. Both quantitative and qualitative data were used together; therefore tringulation method was used as data collection method. In the first level of the study, since it was aimed that instructors evaluate CSA by their own expressions, a semi-constructed and nondirective interviewing technique was used. While analyzing data “descriptive analysis” and “content analysis” approaches were used. At the end of the analysis of the data collected at first stage, it was detected that instructors encounter with some problems during CSA course and they consider CSA course important for students’ personal and social

  12. Nonlinear resonance in Duffing oscillator with fixed and integrative time-delayed feedbacks

    Indian Academy of Sciences (India)

    V Ravichandran; V Chinnathambi; S Rajasekar

    2012-03-01

    We study the nonlinear resonance, one of the fundamental phenomena in nonlinear oscillators, in a damped and periodically-driven Duffing oscillator with two types of time-delayed feedbacks, namely, fixed and integrative. Particularly, we analyse the effect of the time-delay parameter and the strength of the time-delayed feedback. Applying the perturbation theory we obtain a nonlinear equation for the amplitude of the periodic response of the system. For a range of values of and , the response amplitude is found to be higher than that of the system in the absence of delayed feedback. The response amplitude is periodic on the parameter with period 2 / where is the angular frequency of the external periodic force. We show the occurrence of multiple branches of the response amplitude curve with and without hysteresis.

  13. Návrh kompozitového směrového kormidla pro letoun L 410 NG

    OpenAIRE

    Sokol, Filip

    2015-01-01

    Tato diplomová práce se zabývá návrhem kompozitní konstrukce směrového kormidla letounu L410 NG vycházející ze současné duralově-plátnové konstrukce. Podle předpisu FAR 23 a jeho oběžníků je navržen postup certifikace kompozitní primární konstrukce letounu. Je navržena konstrukce nového směrového kormidla a z vybraných materiálů proveden návrh skladby kompozitních materiálů. Programem MSC Patran je z této konstrukce vytvořen MKP model a pomocí softwarů Compost a Nastran provedena pevnostní ko...

  14. Effects of Dăoyĭn Qìgōng in postpolio syndrome patients with cold intolerance

    Directory of Open Access Journals (Sweden)

    Paulo Eduardo Ramos

    2012-09-01

    Full Text Available Postpolio syndrome (PPS is characterized by progressive muscle weakness due to former infection with poliomyelitis and can be associated with other symptoms such as cold intolerance (CI. Dăoyĭn Qìgōng (DQ is a technique in Traditional Chinese Medicine that impacts the circulation of energy and blood. OBJECTIVE: It was to verify the effects of DQ in PPS patients complaining of cold intolerance. METHODS: Ten PPS patients were assessed using the visual analogue scale (VAS adapted for CI before and after intervention with DQ; patients practiced it in a sitting position for 40 minutes, 3 times per week over 3 consecutive months. Patients were reassessed three months after ceasing DQ. RESULTS: There was a statistically significant difference in local and systemic VAS-Cold both at the end of DQ training and three months past the end of this. CONCLUSION: The DQ technique ameliorated CI complaints in patients with PPS.

  15. NG2 cells response to axonal alteration in the spinal cord white matter in mice with genetic disruption of neurofilament light subunit expression

    OpenAIRE

    Xiao Zhi; Tang Ya; Wu Ya; Bao Zhen; He Bei

    2008-01-01

    Abstract Background Chondroitin sulphate proteoglycan (NG2) expressing cells, morphologically characterized by multi-branched processes and small cell bodies, are the 4th commonest cell population of non-neuronal cell type in the central nervous system (CNS). They can interact with nodes of Ranvier, receive synaptic input, generate action potential and respond to some pathological stimuli, but the function of the cells is still unclear. We assumed the NG2 cells may play an active role in neur...

  16. NG Kunstikonteiner alustab kirjandusega

    Index Scriptorium Estoniae

    2008-01-01

    Kunstnik Sandra Jõgeva korraldab Tallinnas kultuuritehases Polymer alternatiivgaleriis Non Grata Kunstikonteiner kirjanduslike kohtumiste seeria "Üks hullem kui teine". Kirjandusüritused ei ole mõeldud ainult oma ala insider'itele. 17. juulil esinevad Armin Kõomägi teemal "Kirjandus ja äri", Andrus Elbing "Vangla sinus eneses" jt. Vt. ka Looming, 2008, nr. 8, lk. 1271

  17. ng meediaga

    Index Scriptorium Estoniae

    2006-01-01

    Ajakirjanduse ja ettevõtete vahelistest suhetest maine kujundamises. Art. allikaks on Peter Vanderwickeni 2005. aasta jaanuaris Harvard Business Review's ilmunud lugu "Why the news is not the truth". Kommenteerivad Priit Pullerits ja Neeme Raud

  18. Theoretical study of C−H⋯H−B dihydrogen bonded complexes between inert molecules FNgCCH (Ng = Ar and Kr) and borane-amines

    International Nuclear Information System (INIS)

    Graphical abstract: C−H⋯H−B dihydrogen bonding in inert molecules have been explored by studying FNgCCH (Ng = Ar, Kr) complexes with BH3–NH3, BH3–NH2Me and BH3–NHMe2 at MP2/6-311++G(d,p) and MP2/aug-cc-pVDZ level of theories. Further, strength of hydrogen bonding and dihydrogen bonding in inert complexes have been compared under identical condition and it has been found that strength of dihydrogen bonded complexes are ∼15% weaker as compared to hydrogen bonded complexes. Highlights: ► C–H⋯H–B dihydrogen bonding ability of rare gas inserted FNgCCH molecules. ► Comparison of strength of hydrogen bond and dihydrogen bond for inert complexes. ► Dihydrogen bonding is 15% weaker as compare to hydrogen bonding. - Abstract: C−H⋯H−B dihydrogen bonding in inert molecules have been explored by studying FNgCCH (Ng = Ar, Kr) complexes with BH3–NH3, BH3–NH2Me and BH3–NHMe2 at MP2/6-311++G(d,p) and MP2/aug-cc-pVDZ level of theories. The H⋯H contact distances are found to be less than 2.4 Å while binding energies are in the range of 15–18 kJ mol−1. Furthermore, changes in C–H and B–H stretching frequencies authenticate to the formation of C−H⋯H−B dihydrogen bonding in these complexes. Moreover, properties of C−H⋯H−B dihydrogen bonding have also been supplemented by molecular electrostatic potential derived charge, natural population, natural bond order and atoms in molecules analysis. Strength of hydrogen bonding and dihydrogen bonding in inert complexes have been compared under identical condition and it has been found that strength of dihydrogen bonded complexes are ∼15% weaker as compared to hydrogen bonded complexes.

  19. Subcritical assemblies with fast and thermal neutron spectrum driven with high intensity neutron generator NG-12-1

    International Nuclear Information System (INIS)

    Full text: The investigations in the field of nuclear physics, development of numerical calculation methods for nuclear reactors, neutron physics and etc. are carried out at the Joint Institute for Power and Nuclear Research - Sosny (JIPNR-Sosny) since the 60s after putting into operation the research reactor and the critical assemblies. A large range of different configuration (geometry,composition) of critical assemblies have been constructed at the NAS Belarus during 25 years of studding neutronic of the special (fast and thermal) reactors. The Chernobyl accident brought a massive public reaction to nuclear efforts and the reactor ceased operation in 1987 and was shut down in 1991 and all investigations using of the reactor neutrons were interrupted. Closure of the reactor led to new endeavors being undertaken at NAS Belarus including the coupling of a high-yield neutron generator to a sub-critical assemblies fuelled with high enriched uranium. The neutron generator NG-12-1 consists of a high-current deuteron accelerator, highly effective water-cooling rotating Ti3H (TiD) 230 mm diameter target and has been operated since 1997 as intense continuous neutron source of (1.5-2.0) 1012 n/s at maximum with neutron energy 13.0 - 15.0 MeV and a continuous neutron source of (2.0-3.0) 1010 n/s at maximum with neutron energy 2.0- 3.0 MeV. When operating in the pulse mode the neutron beam pulse can be adjusted from 0.5μs up to 100μs and pulse repetition rate can be vary from 1 Hz to 10 000 Hz. According research program of the NAS of Belarus the experimental facility 'Yalina' consisting of the sub-critical assembly with neutron thermal spectrum (source neutron multiplication factor Ms of the assembly is in the range of 10 ≤ Ms ≥ 50), high intensity neutron generator and measurement systems was developed and put into operation in 2001. The core of the subcritical assembly is a rectangular parallelepiped 40.0cm width, 40.0cm length and 57.0 cm height. It is assembled

  20. Determination of silver at ng mL-1 levels by preconcentration on a W-coil trap and hydride generation atomic absorption spectrometry

    International Nuclear Information System (INIS)

    Complete text of publication follows. A classical hydride generation atomic absorption spectrometry flow system consists of a reaction coil, a stripping coil, a gas liquid separator (GLS) and a heated quartz T-tube atomizer. There have been some studies for determination of Ag by using this classical hydride generation system; however, only mg L-1 levels could be determined. In this study, three types of gas-liquid separators, namely, U-shape, cylindrical and a novel large volume device were used. It was possible to determine Ag in ng mL-1 level when stripping coil was eliminated from the system. The calibration plot was linear in the range of 50 to 500 ng mL-1 using a U-shape GLS. The 3s limit of detection (LOD) is 29 ng mL-1 and characteristic concentration value, C0, were found to be 15.3 ng mL-1. The similar set-up was used also with a cylindrical GLS; it was observed that the efficiency of this GLS is about 10 fold higher than the U-shape GLS; linear working range was 5.0 to 50 ng mL-1. LOD (3s) and C0 values were found to be 3.0 ng mL-1 and 2.9 ng mL-1, respectively. In order to minimize the memory effects, a 10% (v/v) solution of dichlorodimethylsilane in toluene was used to silanize both gas liquid separators to deactivate their internal surfaces. In addition to these studies, a novel large volume GLS was used. In this device 15.0 mL of mixed acidic analyte solution and NaBH4 were placed. Argon gas was passed through this mixture for 90 seconds and formed volatile analyte species were sent to a W-coil trap heated at an optimized temperature. The calibration plot was linear between 0.10 and 0.75 ng mL-1; the LOD (3s) and C0 were found to be 0.049 ng mL-1 and 0.050 ng mL-1, respectively.

  1. Neuroimaging of the bilingual brain: evidence and research methodology = Neuroimagem do cérebro bilíngüe: evidências e metodologia de pesquisa

    Directory of Open Access Journals (Sweden)

    Ansaldo, Ana Inés et. al.

    2008-01-01

    Full Text Available Atualmente mais da metade da população mundial é bilíngüe, sendo o estudo do processamento lingüístico e de seus correlatos cerebrais em indivíduos bilíngües um tópico relevante e inovador na recente área da neuropsicolingüística. Este artigo tem dois objetivos: 1 discutir alguns aspectos metodológicos relacionados ao desenvolvimento de investigações com técnicas de neuroimagem sobre o processamento da linguagem no cérebro bilíngüe e 2 revisar evidências de neuroimagem sobre o processamento lingüístico considerando fatores individuais que têm caracterizado a literatura acerca do bilingüismo, por seu importante papel na representação cerebral bilíngüe da linguagem. Muitos estudos têm mostrado dados sobre a relação entre idade de aquisição, nível de proficiência, idade cronológica, uso de linguagem e forma de exposição às línguas na organização da linguagem no cérebro bilíngüe. A grande variabilidade de métodos de investigação e um controle metodológico nem sempre criterioso tem tornado difícil a generalização de conclusões sobre uma circuitaria cerebral da linguagem em bilíngües. A presente discussão deve contribuir para o debate dos fundamentos teóricos e metodológicos da organização neurofuncional da linguagem no cérebro bilíngüe.

  2. Nitric Oxide Synthase Inhibition by NG-Nitro-l-Arginine Methyl Ester Inhibits Tumor-Induced Angiogenesis in Mammary Tumors

    Science.gov (United States)

    Jadeski, Lorraine C.; Lala, Peeyush K.

    1999-01-01

    Using a murine breast cancer model, we earlier found a positive correlation between the expression of nitric oxide synthase (NOS) and tumor progression; treatment with inhibitors of NOS, NG-methyl-l-arginine (NMMA) and NG-nitro-l-arginine methyl ester (L-NAME), had antitumor and antimetastatic effects that were partly attributed to reduced tumor cell invasiveness. In the present study, we used a novel in vivo model of tumor angiogenesis using subcutaneous implants of tumor cells suspended in growth factor-reduced Matrigel to examine the angiogenic role of NO in a highly metastatic murine mammary adenocarcinoma cell line. This cell line, C3L5, expresses endothelial (e) NOS in vitro and in vivo, and inducible (i) NOS in vitro on stimulation with lipopolysaccharide and interferon-γ. Female C3H/HeJ mice received subcutaneous implants of growth factor-reduced Matrigel inclusive of C3L5 cells on one side, and on the contralateral side, Matrigel alone; L-NAME and D-NAME (inactive enantiomer) were subsequently administered for 14 days using osmotic minipumps. Immediately after sacrifice, implants were removed and processed for immunolocalization of eNOS and iNOS proteins, and measurement of angiogenesis. Neovascularization was quantified in sections stained with Masson’s trichrome or immunostained for the endothelial cell specific CD31 antigen. While most tumor cells and endothelial cells expressed immunoreactive eNOS protein, iNOS was localized in endothelial cells and some macrophages within the tumor-inclusive implants. Measurable angiogenesis occurred only in implants containing tumor cells. Irrespective of the method of quantification used, tumor-induced neovascularization was significantly reduced in L-NAME-treated mice relative to those treated with D-NAME. The quantity of stromal tissue was lower, but the quantity of necrotic tissue higher in L-NAME relative to D-NAME-treated animals. The total mass of viable tissue (ie, stroma and tumor cells) was lower in L

  3. The critical role of partially exposed N-terminal valine residue in stabilizing GH10 xylanase from Bacillus sp.NG-27 under poly-extreme conditions.

    Directory of Open Access Journals (Sweden)

    Amit Bhardwaj

    Full Text Available BACKGROUND: Understanding the mechanisms that govern protein stability under poly-extreme conditions continues to be a major challenge. Xylanase (BSX from Bacillus sp. NG-27, which has a TIM-barrel structure, shows optimum activity at high temperature and alkaline pH, and is resistant to denaturation by SDS and degradation by proteinase K. A comparative circular dichroism analysis was performed on native BSX and a recombinant BSX (R-BSX with just one additional methionine resulting from the start codon. The results of this analysis revealed the role of the partially exposed N-terminus in the unfolding of BSX in response to an increase in temperature. METHODOLOGY: We investigated the poly-extremophilicity of BSX to deduce the structural features responsible for its stability under one set of conditions, in order to gain information about its stability in other extreme conditions. To systematically address the role of the partially exposed N-terminus in BSX stability, a series of mutants was generated in which the first hydrophobic residue, valine (Val1, was either deleted or substituted with various amino acids. Each mutant was subsequently analyzed for its thermal, SDS and proteinase K stability in comparison to native BSX. CONCLUSIONS: A single conversion of Val1 to glycine (Gly changed R-BSX from being thermo- and alkali- stable and proteinase K and SDS resistant, to being thermolabile and proteinase K-, alkali- and SDS- sensitive. This result provided insight into the structure-function relationships of BSX under poly-extreme conditions. Molecular, biochemical and structural data revealed that the poly-extremophilicity of BSX is governed by a partially exposed N-terminus through hydrophobic interactions. Such hitherto unidentified N-terminal hydrophobic interactions may play a similar role in other proteins, especially those with TIM-barrel structures. The results of the present study are therefore of major significance for protein folding

  4. The Escape of Sisyphus or What “Post NG-PON2” Should Do Apart from Neverending Capacity Upgrades

    Directory of Open Access Journals (Sweden)

    Martin Maier

    2014-03-01

    Full Text Available The primary design goal of (revolutionary NG-PON1&2 was the provisioning of an ever increasing capacity to cope with video-dominated traffic and handle the explosion of mobile data traffic by means of offloading. Recently, however, questions on the future of “post NG-PON2” have surfaced whether to shift its research focus to business and operation related aspects and move access technology into a substantially different direction than continued capacity upgrades. In fact, recent studies indicate that ultimately the major factor limiting the performance of 4G mobile networks is latency rather than capacity of the backhaul. In this paper, we review recently proposed low-latency techniques for NG-PONs that require architectural modifications at the remote node or distribution fiber level and highlight advanced network coding and real-time polling based low-latency techniques that can be implemented in software, enable NG-PONs to carry higher traffic loads and thereby extend their lifetime, and maintain the passive nature of existent optical distribution networks. Furthermore, we elaborate on emerging trends and open challenges for future post NG-PON2 research. To better understand their true potential, we put them into a wider non-technical and historical perspective leading up to a sustainable Third Industrial Revolution (TIR economy and its underlying Energy Internet.

  5. Myocilin is involved in NgR1/Lingo-1-mediated oligodendrocyte differentiation and myelination of the optic nerve.

    Science.gov (United States)

    Kwon, Heung Sun; Nakaya, Naoki; Abu-Asab, Mones; Kim, Hong Sug; Tomarev, Stanislav I

    2014-04-16

    Myocilin is a secreted glycoprotein that belongs to a family of olfactomedin domain-containing proteins. Although myocilin is detected in several ocular and nonocular tissues, the only reported human pathology related to mutations in the MYOCILIN gene is primary open-angle glaucoma. Functions of myocilin are poorly understood. Here we demonstrate that myocilin is a mediator of oligodendrocyte differentiation and is involved in the myelination of the optic nerve in mice. Myocilin is expressed and secreted by optic nerve astrocytes. Differentiation of optic nerve oligodendrocytes is delayed in Myocilin-null mice. Optic nerves of Myocilin-null mice contain reduced levels of several myelin-associated proteins including myelin basic protein, myelin proteolipid protein, and 2'3'-cyclic nucleotide 3'-phosphodiesterase compared with those of wild-type littermates. This leads to reduced myelin sheath thickness of optic nerve axons in Myocilin-null mice compared with wild-type littermates, and this difference is more pronounced at early postnatal stages compared with adult mice. Myocilin also affects differentiation of oligodendrocyte precursors in vitro. Its addition to primary cultures of differentiating oligodendrocyte precursors increases levels of tested markers of oligodendrocyte differentiation and stimulates elongation of oligodendrocyte processes. Myocilin stimulation of oligodendrocyte differentiation occurs through the NgR1/Lingo-1 receptor complex. Myocilin physically interacts with Lingo-1 and may be considered as a Lingo-1 ligand. Myocilin-induced elongation of oligodendrocyte processes may be mediated by activation of FYN and suppression of RhoA GTPase. PMID:24741044

  6. Nearly 1000 Protein Identifications from 50 ng of Xenopus laevis Zygote Homogenate Using Online Sample Preparation on a Strong Cation Exchange Monolith Based Microreactor Coupled with Capillary Zone Electrophoresis.

    Science.gov (United States)

    Zhang, Zhenbin; Sun, Liangliang; Zhu, Guijie; Cox, Olivia F; Huber, Paul W; Dovichi, Norman J

    2016-01-01

    A sulfonate-silica hybrid strong cation exchange monolith microreactor was synthesized and coupled to a linear polyacrylamide coated capillary for online sample preparation and capillary zone electrophoresis-tandem mass spectrometry (CZE-MS/MS) bottom-up proteomic analysis. The protein sample was loaded onto the microreactor in an acidic buffer. After online reduction, alkylation, and digestion with trypsin, the digests were eluted with 200 mM ammonium bicarbonate at pH 8.2 for CZE-MS/MS analysis using 1 M acetic acid as the background electrolyte. This combination of basic elution and acidic background electrolytes results in both sample stacking and formation of a dynamic pH junction. 369 protein groups and 1274 peptides were identified from 50 ng of Xenopus laevis zygote homogenate, which is comparable with an offline sample preparation method, but the time required for sample preparation was decreased from over 24 h to less than 40 min. Dramatically improved performance was produced by coupling the reactor to a longer separation capillary (∼100 cm) and a Q Exactive HF mass spectrometer. 975 protein groups and 3749 peptides were identified from 50 ng of Xenopus protein using the online sample preparation method. PMID:26670623

  7. Photo-dynamics of the lyophilized photo-activated adenylate cyclase NgPAC2 from the amoeboflagellate Naegleria gruberi NEG-M strain

    Science.gov (United States)

    Penzkofer, A.; Tanwar, M.; Veetil, S. K.; Kateriya, S.; Stierl, M.; Hegemann, P.

    2013-09-01

    The absorption and emission spectroscopic behavior of lyophilized photo-activated adenylate cyclase NgPAC2 from the amoeboflagellate Naegleria gruberi NEG-M strain consisting of a BLUF domain (BLUF = Blue Light sensor Using Flavin) and a cyclase homology domain was studied in the dark, during blue-light exposure and after blue-light exposure at a temperature of 4 °C. The BLUF domain photo-cycle dynamics observed for snap-frozen NgPAC2 was lost by lyophilization (no signaling state formation with flavin absorption red-shift). Instead, blue-light photo-excitation of lyophilized NgPAC2 caused sterically restricted Tyr-Tyr cross-linking (o,o‧-ditysosine formation) and partial flavin cofactor reduction.

  8. Polysialic acid on SynCAM 1 in NG2 cells and on neuropilin-2 in microglia is confined to intracellular pools that are rapidly depleted upon stimulation.

    Science.gov (United States)

    Werneburg, Sebastian; Mühlenhoff, Martina; Stangel, Martin; Hildebrandt, Herbert

    2015-07-01

    NG2 cells comprise a heterogeneous precursor population but molecular markers distinguishing between the assumed NG2 cell subpopulations are lacking. Previously, we described that a subfraction of the synaptic cell adhesion molecule SynCAM 1 is modified with the glycan polysialic acid (polySia) in NG2 cells. As for its major carrier, the neural cell adhesion molecule NCAM, polySia attenuates SynCAM 1 adhesion. Functions, as well as cellular and subcellular distribution of polySia-SynCAM 1 are elusive. Using murine glial cultures we now demonstrate that polySia-SynCAM 1 is confined to the Golgi compartment of a subset of NG2 cells and transiently recruited to the cell surface in response to depolarization. NG2 cells with Golgi-confined polySia were NCAM-negative, but positive for markers of oligodendrocyte precursor cells (OPCs). Consistent with previous data on polySia-SynCAM 1, polySia in Ncam(-/-) NG2 cells was exclusively attached to N-glycans and synthesized by ST8SIA2, one out of two mammalian polysialyltransferases. Unexpectedly, Golgi-confined polySia was also detected in Ncam(-/-) microglia, but this fraction resided on O-glycans and was produced by the second polysialyltransferase, ST8SIA4, indicating the presence of yet another polySia carrier in microglia. Searching for this carrier, we identified polysialylated neuropilin-2, so far only known from dendritic cells and exudate macrophages. Microglia activation by LPS, but not interleukin-4, caused a transient translocation of Golgi-localized polySia to the cell surface, resulting in complete depletion. Finally, NO-production of LPS-stimulated microglia was attenuated by addition of polySia suggesting that the observed loss of polySia-neuropilin-2 is involved in negative feedback regulation of pro-inflammatory microglia polarization. PMID:25752299

  9. Rapid measurement of perchlorate in polar ice cores down to sub-ng L(-1) levels without pre-concentration.

    Science.gov (United States)

    Peterson, Kari; Cole-Dai, Jihong; Brandis, Derek; Cox, Thomas; Splett, Scott

    2015-10-01

    An ion chromatography-electrospray ionization-tandem mass spectrometry (IC-ESI-MS/MS) method has been developed for rapid and accurate measurement of perchlorate in polar snow and ice core samples in which perchlorate concentrations are expected to be as low as 0.1 ng L(-1). Separation of perchlorate from major inorganic species in snow is achieved with an ion chromatography system interfaced to an AB SCIEX triple quadrupole mass spectrometer operating in multiple reaction monitoring mode. Under optimized conditions, the limit of detection and lower limit of quantification without pre-concentration have been determined to be 0.1 and 0.3 ng L(-1), respectively, with a linear dynamic range of 0.3-10.0 ng L(-1) in routine measurement. These represent improvements over previously reported methods using similar analytical techniques. The improved method allows fast, accurate, and reproducible perchlorate quantification down to the sub-ng L(-1) level and will facilitate perchlorate measurement in the study of natural perchlorate production with polar ice cores in which perchlorate concentrations are anticipated to vary in the low and sub-ng L(-1) range. Initial measurements of perchlorate in ice core samples from central Greenland show that typical perchlorate concentrations in snow dated prior to the Industrial Revolution are about 0.8 ng L(-1), while perchlorate concentrations are significantly higher in recent (post-1980) snow, suggesting that anthropogenic sources are a significant contributor to perchlorate in the current environment. PMID:26297465

  10. Impacts of agricultural intensification through upscaling of suitable rainwater harvesting technologies in the upper Ewaso Ng'iro North basin, Kenya

    Directory of Open Access Journals (Sweden)

    J. K. Mutiga

    2011-03-01

    Full Text Available Changes in land cover and land use can lead to significant impacts to hydrology by affecting the amount of runoff, soil moisture and groundwater recharge over a range of temporal and spatial scales. However, hydrologic effects of these changes are still an unknown at watershed scale. Moreover, predicting the effects of land cover/use and climate change on hydrological cycle has remained a major challenge. This is because of the complexity and uncertainty of future climate changes making it difficult to predict the consequences. It is against this backdrop that, for sustainable water resources management, assessment of the impacts of land cover/use change on hydrological regime at all scales becomes critical. During this study, we applied the SWAT model to assess the impacts of area hydrology between baseline and alternative scenario (upscaling of rainwater harvesting technologies. Specifically, our overall objective was to quantitatively evaluate the effects of land use changes on watershed hydrology in the upper Ewaso Ng'iro North basin in Kenya. This was achieved by estimating hydrological responses under historical land use scenarios obtained from the multi-temporal satellite imageries of 1987, 1995 and 2003. The model performance was found to be relatively good (Nash and Sutcliffe efficient of 70%. Stream flow analysis was carried out for different parts of the basin to understand its hydrological responses, especially, the behavior of base flow. The results show a decrease in base flow during 1987–2003 period with decreasing forest, bush and grass covers, which can be attributed to poor natural vegetation emanating mainly from overgrazing and deforestation for agricultural activities. In conclusion, the study clearly shows that, assessment of hydrologic effects of land use changes is critical for a sustainable water resources planning and management of the basin.

  11. Preparation and Study of Charge Transfer Complexes of N.N.N.N-Tetra-Methyl-Para-Phenylene-Diamine (TMPD with NG, Tetryl, RDX and HMX

    Directory of Open Access Journals (Sweden)

    K. Bahadur

    1983-04-01

    Full Text Available Charge transfer complexes of NG tetryl, RDX and HMX as electron acceptors with tetra-methyl-para-phenylene-diamine (TMPD as electron donor were prepared and studied. When the solution of nitro-explosives in an inert solvent were added to a solution of TMPD, an intense violet colour was produced. This colour is due to the formation of TMPD cation known as Wurster radical formed from TMPD through the loss of an electron. It has been shown that one mole of each of NG, tetryl, RDX and HMX is needed to form oneTMPD cation. These complexes have also been studied by ultra-violet and infrared spectroscopy.

  12. Förbättrad produktionsplanering med SAP APO : En studie på Siemens Industrial Turbomachinery AB i Finspång

    OpenAIRE

    Walldén, Joel; Elofsson Tottmar, Kristian

    2010-01-01

    Siemens Industrial Turbomachinery AB, nedan utskrivet som Siemens, är en svensk turbintillverkare placerad i Finspång som tillhör den tyska koncernen Siemens AG. Turbintillverkningen i Finspång började 1911 under namnet STAL men har sedan dess bytt ägarstruktur flertalet gånger. STAL-Laval, ABB STAL och Alstom Power är ett urval av de namn verksamheten har haft innan den blev en del av Siemens. Ett problem som Siemens står inför vid produktionsplanering av gasturbiner är de mycket långa ledti...

  13. The Escape of Sisyphus or What “Post NG-PON2” Should Do Apart from Neverending Capacity Upgrades

    OpenAIRE

    Martin Maier

    2014-01-01

    The primary design goal of (r)evolutionary NG-PON1&2 was the provisioning of an ever increasing capacity to cope with video-dominated traffic and handle the explosion of mobile data traffic by means of offloading. Recently, however, questions on the future of “post NG-PON2” have surfaced whether to shift its research focus to business and operation related aspects and move access technology into a substantially different direction than continued capacity upgrades. In fact, recent studies ...

  14. Experimental Demonstration of 6-Mode Division Multiplexed NG-PON2: Cost Effective 40 Gbit/s/Spatial-Mode Access Based on 3D Laser Inscribed Photonic Lanterns

    DEFF Research Database (Denmark)

    Asif, Rameez; Hu, Hao; Mitchell, Paul;

    We report the first space-division-multiplexed based symmetric NG-PON2 network by effi- ciently transmitting 40 Gbit/s/spatial-mode. Error free transmission (BER of 10−9 ) is obtained for all the downstream and upstream data tributaries over 1-km 6-spatial-mode FMF without using MIMO DSP......We report the first space-division-multiplexed based symmetric NG-PON2 network by effi- ciently transmitting 40 Gbit/s/spatial-mode. Error free transmission (BER of 10−9 ) is obtained for all the downstream and upstream data tributaries over 1-km 6-spatial-mode FMF without using MIMO DSP...

  15. Muutumismäng 2010. 1.-5. osa / tekst Kerttu Soans ; kommenteerinud Tõnis Elling, Maiu Herzmann, Reet Martinson, Marek Sildoja, Pille Mitt, Marko Vilipson

    Index Scriptorium Estoniae

    Soans, Kerttu, 1961-

    2010-01-01

    Ajakirja Üks Muutumismäng 2010 jälgib nende käekäiku, kes soovivad langetada kaalu osaledes spordiklubi Artic Sport XL-treeningprogrammis. Tutvustatakse ka inimest, kes on eeskujuks teistele treenijatele ja kaalulangetajatele

  16. Evaluation of the Prostate Imaging Reporting and Data System for Magnetic Resonance Imaging Diagnosis of Prostate Cancer in Patients with Prostate-specific Antigen <20 ng/ml

    Directory of Open Access Journals (Sweden)

    Xuan Wang

    2016-01-01

    Conclusions: The PI-RADS score correlates with the PCa detection rate in patients with PSA <20 ng/ml. The summed score of T2WI + DWI has the highest accuracy in detection of PCa. However, the sensitivity should be further improved.

  17. On the Least-Squares Fitting of Slater-Type Orbitals with Gaussians: Reproduction of the STO-NG Fits Using Microsoft Excel and Maple

    Science.gov (United States)

    Pye, Cory C.; Mercer, Colin J.

    2012-01-01

    The symbolic algebra program Maple and the spreadsheet Microsoft Excel were used in an attempt to reproduce the Gaussian fits to a Slater-type orbital, required to construct the popular STO-NG basis sets. The successes and pitfalls encountered in such an approach are chronicled. (Contains 1 table and 3 figures.)

  18. NG-PON2 Architecture Enabled by Heterogeneous Space Division Multiplexing with Distributed Light Source: A Proof-of-Concept Evaluation

    DEFF Research Database (Denmark)

    Asif, Rameez; Imran, Muhammad; Ye, Feihong;

    2015-01-01

    We have numerically evaluated the next generation passive optical network stage-2 (NG-PON2) architecture based on heterogeneous space division multiplexing (SDM) and wavelength-division multiplexing (WDM). Error free transmission (BER of 10−9 ) is obtained for all the downstream (DS) and upstream...

  19. Comparison of the effects of NG-nitro-L-arginine and indomethacin on the hypercapnic cerebral blood flow increase in rats

    DEFF Research Database (Denmark)

    Wang, Qian; Pelligrino, D A; Paulson, O B;

    1994-01-01

    The effects of NG-nitro-L-arginine (NOLAG), an inhibitor of nitric oxide synthase (NOS), and of indomethacin, an inhibitor of cyclooxygenase, on the rise in cerebral blood flow (CBF) accompanying increasing levels of hypercapnia (paCO2 = 40-135 mmHg) were studied in anesthetized rats. CBF...

  20. Effect of nitric oxide blockade by NG-nitro-L-arginine on cerebral blood flow response to changes in carbon dioxide tension

    DEFF Research Database (Denmark)

    Wang, Qian; Paulson, O B; Lassen, N A

    1992-01-01

    The importance of nitric oxide (NO) for CBF variations associated with arterial carbon dioxide changes was investigated in halothane-anesthetized rats by using an inhibitor of nitric oxide synthase, NG-nitro-L-arginine (NOLAG). CBF was measured by intracarotid injection of 133Xe. In normocapnia...

  1. Comparison of (/sup 125/I)beta-endorphin binding to rat brain and NG108-15 cells using a monoclonal antibody directed against the opioid receptor

    Energy Technology Data Exchange (ETDEWEB)

    Bidlack, J.M.; O' Malley, W.E.; Schulz, R.

    1988-02-01

    The properties of (/sup 125/I)beta h-endorphin-binding sites from rat brain membranes and membranes from the NG108-15 cell line were compared using a monoclonal antibody directed against the opioid receptor and opioid peptides as probes. The binding of (/sup 125/I)beta h-endorphin to both rat brain and NG108-15 membranes yielded linear Scatchard plots with Kd values of 1.2 nM and 1.5 nM, respectively, and Bmax values of 865 fmol/mg rat brain membrane protein and 1077 fmol/mg NG108-15 membrane protein. A monoclonal antibody, OR-689.2.4, capable of inhibiting mu and delta binding but not kappa binding to rat brain membranes, noncompetitively inhibited the binding of 1 nM (/sup 125/I)beta h-endorphin to rat brain and NG108-15 membranes with an IC50 value of 405 nM for rat brain membranes and 543 nM for NG108-15 membranes. The monoclonal antibody also inhibited the binding of 3 nM (/sup 3/H) (D-penicillamine2, D-penicillamine5) enkephalin to NG108-15 membranes with an IC50 value of 370 nM. In addition to blocking the binding of (/sup 125/I)beta h-endorphin to brain membranes, the antibody also displaced (/sup 125/I)beta h-endorphin from membranes. Site-specific opioid peptides had large variations in their IC50 values depending on whether they were inhibiting (/sup 125/I)beta h-endorphin binding to rat brain or the NG108-15 membranes. When the peptides were tested with the monoclonal antibody for their combined ability to inhibit (/sup 125/I)beta h-endorphin binding to both membrane preparations, the peptides and antibody blocked binding as though they were acting at allosterically coupled sites, not two totally independent sites. These studies suggest that mu-, delta-, and beta-endorphin-binding sites share some sequence homology with the 35,000-dalton protein that the antibody is directed against.

  2. NG2 cells response to axonal alteration in the spinal cord white matter in mice with genetic disruption of neurofilament light subunit expression

    Directory of Open Access Journals (Sweden)

    Xiao Zhi

    2008-10-01

    Full Text Available Abstract Background Chondroitin sulphate proteoglycan (NG2 expressing cells, morphologically characterized by multi-branched processes and small cell bodies, are the 4th commonest cell population of non-neuronal cell type in the central nervous system (CNS. They can interact with nodes of Ranvier, receive synaptic input, generate action potential and respond to some pathological stimuli, but the function of the cells is still unclear. We assumed the NG2 cells may play an active role in neuropathogenesis and aimed to determine if NG2 cells could sense and response to the alterations in the axonal contents caused by disruption of neurofilament light subunit (NFL expression. Results In the early neuropathological development stage, our study showed that the diameter of axons of upper motor neurons of NFL-/- mice decreased significantly while the thickness of their myelin sheath increased remarkably. Although there was an obvious morphological distortion in axons with occasionally partial demyelination, no obvious changes in expression of myelin proteins was detected. Parallel to these changes in the axons and their myelination, the processes of NG2 cells were disconnected from the nodes of Ranvier and extended further, suggesting that these cells in the spinal cord white matter could sense the alteration in axonal contents caused by disruption of NFL expression before astrocytic and microglial activation. Conclusion The structural configuration determined by the NFL gene may be important for maintenance of normal morphology of myelinated axons. The NG2 cells might serve as an early sensor for the delivery of information from impaired neurons to the local environment.

  3. Mild hypothermia combined with a scaffold of NgR-silenced neural stem cells/Schwann cells to treat spinal cord injur y

    Institute of Scientific and Technical Information of China (English)

    Dong Wang; Jinhua Liang; Jianjun Zhang; Shuhong Liu; Wenwen Sun

    2014-01-01

    Because the inhibition of Nogo proteins can promote neurite growth and nerve cell differenti-ation, a cell-scaffold complex seeded with Nogo receptor (NgR)-silenced neural stem cells and Schwann cells may be able to improve the microenvironment for spinal cord injury repair. Previ-ous studies have found that mild hypothermia helps to attenuate secondary damage in the spinal cord and exerts a neuroprotective effect. Here, we constructed a cell-scaffold complex consisting of a poly(D,L-lactide-co-glycolic acid) (PLGA) scaffold seeded with NgR-silenced neural stem cells and Schwann cells, and determined the effects of mild hypothermia combined with the cell-scaffold complexes on the spinal cord hemi-transection injury in the T9 segment in rats. Compared with the PLGA group and the NgR-silencing cells+PLGA group, hindlimb motor function and nerve electrophysiological function were clearly improved, pathological changes in the injured spinal cord were attenuated, and the number of surviving cells and nerve ifbers were increased in the group treated with the NgR-silenced cell scaffold+mild hypothermia at 34°C for 6 hours. Furthermore, fewer pathological changes to the injured spinal cord and more surviv-ing cells and nerve ifbers were found after mild hypothermia therapy than in injuries not treated with mild hypothermia. These experimental results indicate that mild hypothermia combined with NgR gene-silenced cells in a PLGA scaffold may be an effective therapy for treating spinal cord injury.

  4. Strategic environmental evaluation of natural gas - SEA/NG: a management tool for development of a environmental conservation policy; Avaliacao ambiental estrategica para o gas natural - AAE/GN: instrumento de gestao do desenvolvimento para uma politica de conservacao ambiental

    Energy Technology Data Exchange (ETDEWEB)

    Comar, Vito; Turdera, Mirko Valenzuela; Silva, Paulo Souza; Benedetti Filho, Edemar; Santiago, Etenaldo Felipe; Costa, Fabio Edir dos Santos [Universidade Estadual do Mato Grosso do Sul (UEMS), Campo Grande, MS (Brazil). Centro de Analise e Monitoramento Ambiental do Gas Natural (GASLAB); Moretti, Edvaldo; Oliveira, Jorge Eremites de; Sciamarelli, Alan; Freitas Filho, Jose Daniel de; Vieira, Maria Helena Pereira [Universidade Estadual do Mato Grosso do Sul (UEMS), Campo Grande, MS (Brazil); Soares, Afranio Jose Soriano [UNESP, Rio Claro, SP (Brazil); Oliveira, Henrique de; Comunello, Eder [EMBRAPA, Dourados, MS (Brazil). Centro de Pesquisas Agropecuaria do Oeste (CPAO); Freire, Luiz G.M. [PETROBRAS, Rio de Janeiro, RJ (Brazil)

    2004-07-01

    The methodology for a Strategic Environmental Evaluation of Natural Gas - SEA/NG incorporates within the analysis of the project the socio-cultural, economic, energetic and environmental implications of the transformations, due to the adoption of natural gas, in the area of influence of gas pipelines and gas-powered thermo-electric plants. It was based on the case study of a gas pipeline planned to reach the city of Dourados from Campo Grande, capital of the Brazilian State of Mato Grosso do Sul, which implied the construction of a thermoelectric plant in Dourados. The SEA/NG: 1. Defines the best alternative track for gas pipelines; 2. Proposes location alternatives for gas-powered thermo-electric plants; 3. Evaluates the cumulative and synergic effects of human activities in the socio-environmental aspects; 4. Studies the dynamics of regional development; 4. Supports urban planning decisions in cities housing gas-powered thermo-electric plants; 5. Proposes a System of Analysis and Environmental Monitoring for Natural Gas. Qualitative-quantitative matrixes were used to evaluate the cumulative and synergic effects of the adoption of natural gas within the regional energy matrix, determine the best alternative track for the gas pipeline proposed from Campo Grande to Dourados, and to identify the best location for a gas-powered thermo-electric plant. The matrixes integrated the areas of geology, hydrology, ecology and socio-economics issues both in the phases of construction and operation of the pipeline. Diagrams and interactive conceptual models were also used to synthesize the principal components involved in the choice of the best gas pipeline alternative track. This project received financial grants from PETROBRAS, MSGAS, TBG and FINEP. (author)

  5. Effects of water chemistry and loading conditions on stress corrosion cracking of cold-rolled 316NG stainless steel in high temperature water

    International Nuclear Information System (INIS)

    Research highlights: → Grain boundary properties of crack tip materials are correlated to cracking path. → Effects of stress intensity factor and overloading are quantified by experiments. → Various dependences of crack growth on electrode potential is quantified and analyzed. - Abstract: The effects of electrode potential, stress intensity factor and loading history on stress corrosion cracking growth of a cold-rolled 316NG stainless steel in 288 oC pure water were investigated. Crack branching and intergranular stress corrosion cracking along random grain boundaries were observed by electron-back scattering diffraction. A strong dependence of crack growth rate on stress intensity factor is observed. A single-cycle overloading produced a retarded transient cracking growth period. The mild inhibiting effect of decreasing electrode potential on crack growth of cold-rolled 316NG SS is analyzed based on the interaction between crack tip mechanics and crack tip oxidation kinetics.

  6. Efficiency of 6- and 12-punctures biopsies to detect prostate cancer in patients with PSA< 10 ng/mL and normal digital rectal examination

    OpenAIRE

    Luiz E. Slongo; Mário C. Sugisawa; Sérgio O. IOSHII; Renato Tâmbara Filho; Luiz C.A. Rocha

    2003-01-01

    OBJECTIVE: Establish the efficiency of 6- and 12-punctures transrectal ultrasound-guided needle biopsies in low risk patients for prostate cancer. Six-punctures (sextant) biopsies were compared to 12-punctures biopsies, assessing which is the best strategy to detect this neoplasm. MATERIALS AND METHODS: Among 240 patients submitted to prostate biopsy, 54 with suspected small and organ-localized tumors (prostatic specific antigen < 10 ng/mL and digital exam of the prostate not suggesting cance...

  7. Endothelium-dependent relaxations in sheep pulmonary arteries and veins: resistance to block by NG-nitro-L-arginine in pulmonary hypertension.

    OpenAIRE

    Kemp, B K; Smolich, J J; Ritchie, B C; Cocks, T. M.

    1995-01-01

    1. The effect of the nitric oxide synthase inhibitor, NG-nitro-L-arginine (L-NOARG), on endothelium-dependent relaxation to a receptor-independent agent, ionomycin, was examined in isolated pulmonary arteries and veins from control, short-term and chronic pulmonary hypertensive sheep. All vessel segments were contracted to optimal levels of active force with endothelin-1 to record endothelium-dependent relaxation. 2. Pulmonary hypertension was induced by continuous pulmonary artery air emboli...

  8. Multi-analyte approach for the determination of ng.l-1 amounts of steroid hormones in unidentified aqueous samples

    OpenAIRE

    Noppe, H.; VERHEYDEN, K; Gillis, W.; Courtheyn, D.; Vanthemsche, P.; De Brabander, H.F.

    2006-01-01

    Since the 70s, many analytical methods for the detection of illegal growth promotors, such as thyreostats, anabolics, ß-agonists and corticosteroids have been developed for a wide range of matrices of animal origin, including meat, fat, organ tissue, urine and faeces. The aim of this study was to develop an analytical method for the determination of ng.l-1 levels of estrogens, gestagens, androgens (EGAs) and corticosteroids in aqueous preparations (i.e. drinking water, drinking water suppleme...

  9. Survey of traditional beliefs in the Hungarian Csángó and Székely ethnomedicine in Transylvania, Romania

    OpenAIRE

    Nóra Papp; Kata Birkás-Frendl; Tímea Bencsik; Szilvia Stranczinger; Dóra Czégényi

    2014-01-01

    Transylvania, part of Romania, has a long-standing culture of Hungarian ethnomedicinal practices. The aim of this study was to review the unexplored ethnopharmacological use of plants, animals and other materials, focusing mainly on the beliefs surrounding them; and compare them with traditional uses from other countries and with scientific literature. An ethnobotanical inventory was conducted among Csángó and Székely Hungarians in three areas of the country between 2007 and 2012. Questionnai...

  10. Is there a role for {sup 11}C-choline PET/CT in the early detection of metastatic disease in surgically treated prostate cancer patients with a mild PSA increase <1.5 ng/ml?

    Energy Technology Data Exchange (ETDEWEB)

    Castellucci, Paolo [University of Bologna, Service of Nuclear Medicine, Department of Haematology-Oncology and Laboratory Medicine, Azienda Ospedaliero-Universitaria di Bologna, Policlinico Sant' Orsola-Malpighi, Bologna (Italy); Azienda Ospedaliero-Unversitaria di Bologna Policlinico Sant' Orsola-Malpighi, UO di Medicina Nucleare, PAD. 30, Bologna (Italy); Fuccio, Chiara; Santi, Ivan; Nanni, Cristina; Allegri, Vincenzo; Montini, Gian Carlo; Ambrosini, Valentina; Boschi, Stefano; Fanti, Stefano [University of Bologna, Service of Nuclear Medicine, Department of Haematology-Oncology and Laboratory Medicine, Azienda Ospedaliero-Universitaria di Bologna, Policlinico Sant' Orsola-Malpighi, Bologna (Italy); Rubello, Domenico; Marzola, Maria Cristina [Sanata Maria della Misericordia Hospital, Department of Nuclear Medicine, Medical Physics, Radiology, Service of Nuclear Medicine, PET/CT Centre, Rovigo (Italy); Schiavina, Riccardo; Martorana, Giuseppe [University of Bologna, Service of Urology, Department of Specialist Surgery and Anaesthesiology, Azienda Ospedaliero-Universitaria di Bologna Policlinico Sant' Orsola-Malpighi, Bologna (Italy)

    2011-01-15

    -guided biopsy in 7 patients with local recurrence, (b) surgery and lymphadenectomy in 3 patients, (c) other targeted imaging procedures (MR or bone scan) in 5 patients and (d) clinical FU lasting a minimum of 12 months and including also a contrast-enhanced CT (CECT), an MR, a bone scan and a repeated {sup 11}C-choline PET/CT in 14 patients. Age, time to biochemical relapse (TTR), initial T staging, Gleason score and trigger PSA were not statistically significant in predicting a positive {sup 11}C-choline PET/CT scan both at univariate and multivariate analysis. Instead, PSA kinetics (PSAdt and PSAvel), N status and anti-androgenic therapy at the time of PET scan were statistically significant predictive factors at univariate analysis. Of note, only PSAdt and initial N status were found to be significant and independent predictive factors at multivariate analysis. The mean PSAdt in PET-positive patients was 4.34 months (SD 2.82) while in PET-negative patients it was 13.30 months (SD 9.75) (p = 0.0001). The optimal threshold for PSAdt established by receiver-operating characteristic (ROC) analysis was 7.25 months (AUC 0.85; 95% confidence interval 0.77-0.91) providing 93% sensitivity, 74% specificity, 60% positive predictive value and 96% negative predictive value. In our study, {sup 11}C-choline PET/CT was able to detect recurrent disease in 28% of the patients with mild biochemical relapse characterized by very low trigger PSA levels (PSA <1.5 ng/ml). Very interestingly {sup 11}C-choline PET/CT detected distant unexpected metastases in 21% of the patients. At multivariate statistical analysis only PSAdt and node status were shown to be significant and independent predictive factors for positive {sup 11}C-choline PET/CT. Therefore, {sup 11}C-choline could be suggested to be performed early during initial biochemical relapse in patients presenting with fast PSA kinetics. The early detection of the site of recurrence could lead to a prompt instauration of the most appropriate

  11. Recent Progress of Research on Herbal Products Used in Traditional Chinese Medicine: the Herbs belonging to The Divine Husbandman's Herbal Foundation Canon (神農本草經 Shén Nóng Běn Cǎo Jīng)

    OpenAIRE

    Lee, Kuo-Hsiung; Morris-Natschke, Susan; Qian, Keduo; Dong, Yizhou; Yang, Xiaoming; Zhou, Ting; Belding, Eileen; Wu, Shou-Fang; Wada, Koji; Akiyama, Toshiyuki

    2012-01-01

    This article will review selected herbal products from Chinese Materia Medica that are used in Traditional Chinese Medicine. The herbs come from the upper, middle, and lower class medicines as listed in The Divine Husbandman's Herbal Foundation Canon (神農本草經 Shén Nóng Běn Cǎo Jīng). The review will focus on the active constituents of the herbs and their bioactivities, with emphasis on the most recent progress in research for the period of 2003 to 2011.

  12. A RESEARCH ON FORMİNG THE TOURİSM DEVELOPMENT LEVEL INDEX OF PROVİNCES IN TURKEY

    OpenAIRE

    Cihan SEÇİLMİŞ; SARI, Yaşar

    2010-01-01

    In this study by using the principal component analysis, provinces' tourism development rank is made on basis of the study. The common intersection of six variables which belongs to tourism is taken and 81 provinces' tourism development rank and the index rates are gathered which forms this ranking of the provinces by using the principal component analysis. After the index rates are ranked, the popular cities in the field of tourism in Turkey is listed in the first six ranks as Antalya, İsta...

  13. Creati ng Customer - Based Brand Equity and Measuring Brand Perception of Tourists who Travel for Thermal Tourism: Balcova Survey

    Directory of Open Access Journals (Sweden)

    Melike Gül

    2015-09-01

    Full Text Available Creating customer-based brand equity is one of the most effective tools for achieving competitive advantage in term of thermal tourism businesses that operating under fierce competition in the last years. The aim of the study is to measure customer-based brand equity perception of customers who accommodate in thermal tourism facilities and identify relationship between brand equity dimensions and size with the other dimensions of each. The survey was conducted on 216 domestic and 184 foreign tourists staying at Balcova Thermal Resort which is located in the Balcova Thermal Destination. Exploratory Factor Analysis and Path Analysis was applied the data. Findings show that there are significant and positive relationship between the size of each customer-based brand equity dimensions. In additions, in order to create ustomer-based brand equity for thermal resorts, some recommendations developed on micro, macro and academic level

  14. F actors A ff ecti ng the Adoption of Internet Banking Amongst IIUM’ students : A Structural Equation Modeling Approach (SEM

    Directory of Open Access Journals (Sweden)

    NABIL HUSSEIN AL - FAHIM

    2012-12-01

    Full Text Available This article examines the factors that determine the internet banking adoptio n amongst International Islamic University Malaysia (IIUM and its causal effects using a theoretical model based on the Technology A cceptance Model (TAM. The research model consists of four exogenous latent constructs, namely, awareness , perceived usefulness, trust and perceived risk and endogenous latent construct namely Internet banking adoption . Data relating to constructs were collected from 200 university’s students in (IIUM and subjected to structural Equation Modeling (SEM analysis. Confirmatory Factor Analysis (CFA was performed to examine the reliability, construct validity, convergent validity and goodness of fit of structural models and measurement models. The hypothesized structural model f i ts the data well . The results show that the significant factor that leads to the adoption of internet banking is perceived usefulness but aw arene ss, trust and risk have negative significant towards the use of internet banking.

  15. Framgång, med eller utan karriär? : Unga vuxna om karriär i mediabranschen

    OpenAIRE

    Askerlund, Sara; Blomqvist, Lisen

    2015-01-01

    The aim of this study is to enlighten how todays working environment affects young individuals descriptions of how to reach success in work life, now often titled as ”career”. Our purpose has been to analyse how the term career is constituted discursively among young people in jobs characterized by flexibility. The study is based on six qualitative interviews with young adults in the media industry, all between 20-30 years old. By utilising a Foucaultdian discourse analysis we want to expose ...

  16. No differences in metabolic outcomes between nadir GH 0.4 and 1.0 ng/mL during OGTT in surgically cured acromegalic patients (observational study).

    Science.gov (United States)

    Ku, Cheol Ryong; Choe, Eun Yeong; Hong, Jae Won; Kim, Eui Hyun; Park, Se Hee; Kim, Sun Ho; Lee, Eun Jig

    2016-06-01

    Metabolic impairment is the common cause for mortality in acromegalic patients. In this study, long-term improvements of metabolic parameters were evaluated according to 2 different remission criteria.This was an observational cohort study before and up to 1 year after transsphenoidal adenomectomy (TSA). Participants were 187 patients with acromegaly. At 6 months after TSA, remitted patients with age- and sex-matched normalized IGF-1 were divided into 2 groups: remission 1 (R1), nadir growth hormone (GH) below 0.4 ng/mL; and remission 2 (R2), nadir GH between 0.4 and 1.0 ng/mL in oral glucose tolerance test (OGTT). Metabolic parameters during serial OGTTs were evaluated for 12 months. Remission was achieved in 157 (R1-136; R2-21) patients. Immediate postoperative metabolic parameters including body weight, body mass index, glucose, insulin, and free fatty acid in OGTT were all significantly improved in R1 and R2. HOMA-%β and HOMA-IR scores also improved in both R1 and R2. These improvements persisted for duration (12 months) of this study. However, no difference was present in metabolic parameters between R1 and R2. Although the patients with preoperative adrenal insufficiency presented significantly increased HOMA scores before TSA, there was no difference between classifications of deficient pituitary axes and changes of metabolic parameters after TSA. Remitted patients exhibited rapid restoration of metabolic parameters immediate postoperative period. Long-term improvements in metabolic parameters were not different between the 2 different nadir GH cut-offs, 0.4 and 1.0 ng/mL. PMID:27310957

  17. Development of the Next-Generation Caustic-Side Solvent Extraction (NG-CSSX) Process for Cesium Removal from High-Level Tank Waste

    International Nuclear Information System (INIS)

    This paper describes the chemical performance of the Next-Generation Caustic-Side Solvent Extraction (NG-CSSX) process in its current state of development for removal of cesium from the alkaline high-level tank wastes at the Savannah River Site (SRS) in the US Department of Energy (USDOE) complex. Overall, motivation for seeking a major enhancement in performance for the currently deployed CSSX process stems from needs for accelerating the cleanup schedule and reducing the cost of salt-waste disposition. The primary target of the NG-CSSX development campaign in the past year has been to formulate a solvent system and to design a corresponding flowsheet that boosts the performance of the SRS Modular CSSX Unit (MCU) from a current minimum decontamination factor of 12 to 40,000. The chemical approach entails use of a more soluble calixarene-crown ether, called MaxCalix, allowing the attainment of much higher cesium distribution ratios (DCs) on extraction. Concurrently decreasing the Cs-7SB modifier concentration is anticipated to promote better hydraulics. A new stripping chemistry has been devised using a vitrification-friendly aqueous boric acid strip solution and a guanidine suppressor in the solvent, resulting in sharply decreased DCs on stripping. Results are reported herein on solvent phase behavior and batch Cs distribution for waste simulants and real waste together with a preliminary flowsheet applicable for implementation in the MCU. The new solvent will enable MCU to process a much wider range of salt feeds and thereby extend its service lifetime beyond its design life of three years. Other potential benefits of NG-CSSX include increased throughput of the SRS Salt Waste Processing Facility (SWPF), currently under construction, and an alternative modular near-tank application at Hanford.

  18. DETERM INI NG THE FACTORS WH I CH CAN PREVENT RECREAT I ONAL PART ICIPAION OF UNI VERS I TY STUDENTS WHO A TTEND TO THE DEPARTMENTS ACCORDI NG TO SPEC I AL SK I LL EXAMS

    Directory of Open Access Journals (Sweden)

    Ersan TOLUKAN

    2016-07-01

    Full Text Available The a im of this study was to de termine the factors that prevent the students who studies in the Physical Education and Sport, Musical Education and Painting Departments which request aptitude test, from participating into the recreational activities. Population of the research is consti tuted by total 1918 students who were studying at Gazi University in 2009 - 2010 Academic Years in Physical Education and Sport (1205, Musical Education (216 and Art Education Departments (497 which request aptitude test. Totally 498 volunteer participant s constitute the research‟s sample; 221 students from Physical Education and Sport, 116 students from Musical Education and 161 students from Art Education Departments. Working as a data collection tool and a total of 27 items of the six sub - dime nsions "Le isure Barriers Scale " is used.6 subscales of the questionnaire used in the study to test gender differences in the independent samples t - test analysis was conducted based on the results; by gender "individual psychology in" the (p<0.05 were found to be si gnificant differences . In addition, the ANOVA results, according to the welfare level (individual psychology and lack of interest and section variables and leisure difficulty according to their (individual psychology, lack of friends and lack of interest were statistically significant d ifferences that were observed ( p<0.05 . Consequent ly, the "lack of interest", " facilities/ lack of service " and "individual psychology" factor in preventing the individuals to participate in recreational activities has be en determined that the first three ranks.

  19. Thermodynamics of Modifying Effect of Rare Earth Oxide on Inclusions in Hardfaci ng Metal of Medium-High Carbon Steel

    Institute of Scientific and Technical Information of China (English)

    杨庆祥; 赵研辉; 李艳丽; 姚枚

    2002-01-01

    The modifying effect of rare earth (RE) oxide on inclusions in hardfacing metals of medium-high carbon steel was investigated by means of thermodynamics. The t hermodynamic analysis for inclusion formation shows that RE oxide can be redu ced to RE element by carbon, then the RE element can react with oxygen and sulfu r to form the RE oxide, RE sulfide and RE oxide-sulfide in hardfacing molten po ol. The deoxidization and the desulphurization can be carried out and the liquid metal can be purified. In addition, RE oxide can also react with sulfur to form RE oxide-sulfide directly. Therefore, the harmful effect of sulfur can be decr eased.

  20. Relationship between Statistical and Dynamical properties of fragments produced at Fermi Energy in Heavy ion collisions: ng

    International Nuclear Information System (INIS)

    The properties of the fragments produced in heavy-ion collisions around the Fermi energy have been studied through the isospin degree of freedom. First, a theoretical approach based on a lattice gas model with two types of particles (neutron,proton) interacting by an isospin dependent and Coulomb interactions was developed. The study of the phase diagram shows that this system presents three different phases (liquid, gas, fission). In the liquid and gas phases, the energy of the system was described by a density functional, where the temperature dependence acts only on the density. The symmetry term of this functional was related to the isotopic content of the biggest fragment via an iso-scaling analysis. Secondly a systematic study of the stopping power of the nuclear matter and isospin equilibration of light particles in the most violent collisions was carried out using the experimental data taken by the INDRA multidetector at GANIL and GSI. Two stopping power regimes appear; at low energy (< 40 MeV/A) the stopping power decreases with increasing beam energy, whereas at high energy the stopping power is governed by the quantity of matter along the beam direction. An other study has been focused on the Xe+Sn reaction at 32 and 45 MeV/A with different isospin systems. The separation of three different reaction mechanisms by use of a principal component analysis allowed us to observe that the isospin content of light particles seems to be independent on the mechanism, but depends on the violence of the collision (i.e. impact parameter). (author)

  1. Depression of high-threshold calcium currents by activation of human D2 (short) dopamine receptors expressed in differentiated NG108-15 cells.

    OpenAIRE

    Seabrook, G. R.; McAllister, G.; Knowles, M. R.; Myers, J.; Sinclair, H; Patel, S.; Freedman, S B; Kemp, J.A.

    1994-01-01

    1. This study examined the regulation of calcium currents in differentiated NG108-15 cells that had been stably transfected with cDNA encoding the short isoform of the human D2 dopamine receptor. Whole cell calcium currents were recorded by nystatin-perforated patch clamp recording. 2. Transient low-threshold calcium currents elicited by depolarizations from -100 mV to -20 mV were reversibly depressed by NiCl2 (84 +/- 8% at 30 microM; n = 3) and by omega-agatoxin IVA (15 +/- 5%; 100 nM, n = 7...

  2. Draped heterogeneity, forced uniformity: when agro-environmental policies drive family development: The U Minh Thượng forest reserve, (Mekong delta, Vietnam)

    OpenAIRE

    Saqalli, Mehdi; Dosso, Mireille

    2011-01-01

    The U Minh Thượng natural reserve was created during the 1990s with a surrounding buffer zone where 3,500 households have been settled between 1992 and 1995, each household on a lot of 4 hectares. From a social point of view, the settlers were selected as 'poor people' or war veterans. A social discrimination was slowly built in favour of the latter. Despite an apparent homogeneity, the environment shows slight variations which has huge effects on the potential of the lots. From 1992 to 1998,...

  3. Dissociation of castor oil-induced diarrhoea and intestinal mucosal injury in rat: effect of NG-nitro-L-arginine methyl ester.

    OpenAIRE

    Capasso, F; Mascolo, N; Izzo, A A; Gaginella, T S

    1994-01-01

    1. Castor oil (2 ml orally) produced diarrhoea in rats 1-7 h after challenge, which was associated with gross damage to the duodenal and jejunal mucosa. 2. The injury was accompanied by release of acid phosphatase into the gut lumen, indicating cellular injury. 3. Intraperitoneal injection of the nitric oxide (NO) synthase inhibitor NG-nitro-L-arginine methyl ester (L-NAME, 2.5-50 mg kg-1 twice), prevented the diarrhoea. The dose of L-NAME (50 mg kg-1) completely blocked the diarrhoea but inc...

  4. Inhibition of the release of endothelium-derived relaxing factor in vitro and in vivo by dipeptides containing NG-nitro-L-arginine.

    OpenAIRE

    Thiemermann, C.; Mustafa, M.; Mester, P. A.; Mitchell, J. A.; Hecker, M; Vane, J. R.

    1991-01-01

    1. We have shown that dipeptides containing NG-nitro-L-arginine (NO2Arg) inhibit the biosynthesis of endothelium-derived relaxing factor (EDRF) in vitro and in vivo. 2. In anaesthetized rats, intravenous administration at 1-30 mg kg-1 of the methyl ester of NO2Arg, NO2-Arg-L-phenylalanine (NO2Arg-Phe), L-alanyl-NO2Arg (Ala-NO2Arg) or NO2Arg-L-arginine (NO2Arg-Arg) produced dose-related increases in mean arterial blood pressure (MABP) which were unaffected by D-arginine (D-Arg; 20 mg kg-1 min-...

  5. A GOOD ANTHROPOMETRIC INDEX FOR PREDICTI NG THE RISK OF TYPE 2 DIABETES: WAIST TO HIP RATIO

    Directory of Open Access Journals (Sweden)

    Ramadevi

    2015-07-01

    Full Text Available An increasing trend of type2 Diabetes mellitus is seen with age . Visceral or central obesity is a very common risk factor for developing type 2 diabetes Mellitus. Hence any index which reliably measures obesity can be us ed as a tool to predict the risk of Diabetes. We have attempted to correlate waist to hip ratio (WHR as an anthropometric index to predict the risk of diabetes. When WHR of men is more than 0.9 and women is more than 0.8 the risk increases. MATERIALS & ME THODS: 300 diabetic patients ( 150 males , 150 females and 300 controls (150 males , 150 females were selected and their WHR were measured. The study was done in Gandhi medical college over a period of 2 years. Individuals were grouped according to gender a nd HWR. Data analysis was done by excel sheet and SPSS software. RESULTS: There was significant association between WHR and DM in both males and females. DISCUSSION: Obesity especially visceral leads to insulin resistance and finally DM. This is because adipocytes produce biologic products which modulate insulin action. CONCLUSIONS: Our study proves that HWR screening is a simple and cost effective anthropometric pred ictor of risk for diabetes. This can be used for mass screening as it is quick and needs less expertise.

  6. Contribution of genetic variation rs266882 to prostate-specific antigen levels in healthy controls with serum PSA below 2.0 ng/ml.

    Science.gov (United States)

    Song, Jaeman; Park, Heeyoon; Lee, Gilho

    2013-04-01

    We evaluated the impact of genetic variation in the prostate-specific antigen (PSA) gene (rs266882) on serum PSA levels in healthy men as well as risk factors for benign prostate hypertrophy (BPH) and prostate cancer. The study population comprised 91 men with PSA levels below 2.0 ng/ml as healthy controls, 78 men with PSA 2-10 ng/ml as a BPH group, and 128 prostate cancer patients, all in Korea. DNA was amplified by polymerase chain reaction and the product was sequenced. We found that PSA levels were associated with a G/A polymorphism only in healthy controls. The transition, however, was not associated with PSA levels of BPH and cancer patients, nor was it a risk factor. In conclusion, this genetic factor is important for determining serum PSA levels in the naive group, whereas the disruption of prostatic architecture in BPH or prostate cancer may be a major determining factor for PSA levels. PMID:23315126

  7. Estimation of coolant void reactivity for CANDU-NG lattice using DRAGON and validation using MCNP5 and TRIPOLI-4.3

    International Nuclear Information System (INIS)

    The Coolant Void Reactivity (CVR) is an important safety parameter that needs to be estimated at the design stage of a nuclear reactor. It helps to have an a priori knowledge of the behavior of the system during a transient initiated by the loss of coolant. In the present paper, we have attempted to estimate the CVR for a CANDU New Generation (CANDU-NG) lattice, as proposed at an early stage of the Advanced CANDU Reactor (ACR) development. We have attempted to estimate the CVR with development version of the code DRAGON, using the method of characteristics. DRAGON has several advanced self-shielding models incorporated in it, each of them compatible with the method of characteristics. This study will bring to focus the performance of these self-shielding models, especially when there is voiding of such a tight lattice. We have also performed assembly calculations in 2 x 2 pattern for the CANDU-NG fuel, with special emphasis on checkerboard voiding. The results obtained have been validated against Monte Carlo codes MCNP5 and TRIPOLI-4.3. (authors)

  8. Crystallization and preliminary X-ray study of a family 10 alkali-thermostable xylanase from alkalophilic Bacillus sp. strain NG-27

    International Nuclear Information System (INIS)

    A family 10 alkali-thermostable xylanase from Bacillus sp. NG-27 has been crystallized. A diffraction data set has been collected to 2.2 Å resolution. Xylanases (EC 3.2.1.8) catalyze the hydrolysis of β-1,4-glycosidic linkages within xylan, a major hemicellulose component in the biosphere. The extracellular endoxylanase (XylnA) from the alkalophilic Bacillus sp. strain NG-27 belongs to family 10 of the glycoside hydrolases. It is active at 343 K and pH 8.4. Moreover, it has attractive features from the point of view of utilization in the paper pulp, animal feed and baking industries since it is an alkali-thermostable protein. In this study, XylnA was purified from the native host source and crystallized by the hanging-drop vapour-diffusion method. The crystals belong to the monoclinic space group C2, with unit-cell parameters a = 174.5, b = 54.7, c = 131.5 Å, β = 131.2°, and diffract to better than 2.2 Å resolution

  9. Assay-specific artificial neural networks for five different PSA assays and populations with PSA 2-10 ng/ml in 4,480 men.

    Science.gov (United States)

    Stephan, Carsten; Xu, Chuanliang; Cammann, Henning; Graefen, Markus; Haese, Alexander; Huland, Hartwig; Semjonow, Axel; Diamandis, Eleftherios P; Remzi, Mesut; Djavan, Bob; Wildhagen, Mark F; Blijenberg, Bert G; Finne, Patrik; Stenman, Ulf-Hakan; Jung, Klaus; Meyer, Hellmuth-Alexander

    2007-03-01

    Use of percent free PSA (%fPSA) and artificial neural networks (ANNs) can eliminate unnecessary prostate biopsies. In a total of 4,480 patients from five centers with PSA concentrations in the range of 2-10 ng/ml an IMMULITE PSA-based ANN (iANN) was compared with other PSA assay-adapted ANNs (nANNs) to investigate the impact of different PSA assays. ANN data were generated with PSA, fPSA (assays from Abbott, Beckman, DPC, Roche or Wallac), age, prostate volume, and DRE status. In 15 different ROC analyses, the area under the curve (AUC) in the PSA ranges 2-4, 2-10, and 4-10 ng/ml for the nANN was always significantly larger than the AUC for %fPSA or PSA. The nANN and logistic regression models mostly also performed better than the iANN. Therefore, for each patient population, PSA assay-specific ANNs should be used to optimize the ANN outcome in order to reduce the number of unnecessary biopsies. PMID:17333205

  10. How can the Brazilian emissions legislation influence the size of NG (Natural Gas) light duty vehicles fleet; Como o programa de controle de emissoes veiculares no Brasil pode influenciar a frota de veiculos leves a GNV (Gas Natural Veicular)?

    Energy Technology Data Exchange (ETDEWEB)

    Melo, Tadeu C.C.; Machado, Guilherme B. [PETROBRAS, Rio de Janeiro, RJ (Brazil). Centro de Pesquisas (CENPES); Siqueira, Amanda Albani [Universidade do Estado do Rio de Janeiro (UERJ), Nova Friburgo, RJ (Brazil). Inst. Politecnico

    2004-07-01

    In the last years, a high growth of Brazilian converted Natural Gas (NG) light duty vehicles fleet was observed. It can be related mainly to tax license reduction of NG vehicles; the increase of the NG distribution around the country; attractive price difference between NG and other fuels, mainly gasoline, and an increase on the infrastructure for NG conversion in many places of Brazil. The IBAMA, worried about this uncontrolled increase, published, in 2002, the CONAMA resolution, number 291, that defines ways for the environmental certification of the NG conversion kits and establishes that gas emission from the converted vehicle must be equal or lower than those of the original vehicles, before the conversion. The new PROCONVE phases, which will start in 2007 and 2009, including the requirement for OBD technology (On Board Diagnosis) use and the emission limits reduction, will make the attendance of the legislature difficult to be achieved by the NG conversion companies. This new context can impact on a reduction in the number of converted vehicles and, on the other hand, can stimulate the increase of the OEM participation in this market. (author)

  11. Effects of androgen on phosphacan and NG2 proteoglycan expression and neuronal axial regeneration in traumatic brain injury rat model%雄激素对创伤性脑损伤模型大鼠磷酸蛋白聚糖和NG2蛋白聚糖表达与神经元轴突再生影响的研究

    Institute of Scientific and Technical Information of China (English)

    陈玉红; 仝海波; 段虎斌

    2014-01-01

    目的:了解雄激素对创伤性脑损伤(TBI)大鼠脑组织磷酸蛋白聚糖(PC)及NG2蛋白聚糖表达影响及其与TBI后神经修复的关系,探讨其神经保护及再生的作用机制。方法制作大鼠TBI模型,随机分为正常组、TBI模型组、雄激素干预组(于模型制成后即刻注射丙酸睾丸酮注射液25 mg/kg)。造模后1、3、5、7 d取脑组织制作石蜡切片和电镜切片,用免疫组织化学法观察PC和NG2在损伤区表达的动态变化,应用透射电镜对比观察损伤区及其周围的神经元超微结构变化、细胞损伤、凋亡、神经元轴突再生及胶质细胞增生情况。结果①TBI模型组造模后1 d可见脑细胞肿胀、凋亡,3、7 d细胞水肿,可见凋亡细胞、胶质细胞于损伤外围增生;雄激素干预组1、3、5和7 d时细胞水肿,可见凋亡细胞、损伤外围胶质细胞增生情况明显降低,TBI模型组细胞表面无突起或仅有较小突起;雄激素干预组的神经元轴突较TBI模型组增长明显。②造模后脑组织PC和NG2的表达:正常组脑组织均存在少量PC和NG2的表达;TBI模型组在造模后1 d表达开始呈阳性反应,造模后3 d明显增多,7 d达高峰,但仍高于正常组;雄激素干预组,PC和NG2的表达时间程度及分布与TBI模型组相似,雄激素干预组造模后1、3、5、7 d后损伤区及周围表达水平明显低于TBI模型组,差异均有统计学意义(P<0.01)。结论雄激素可通过调节胶质细胞形态结构和功能抑制PC和NG2表达促进神经元轴突再生,从而发挥神经保护。%Objective To study the effects of androgen on phosphacan (PC) and NG2 proteoglycan (NG2) ex-pression and neuronal regeneration in traumatic brain injury (TBI) rat models, thus unraveling the potential mecha-nisms underlying the protective effects of androgen against TBI. Methods Following construction of TBI models, the rats were randomly

  12. AN INVESTİGATİON OF THE EFFECTİVENESS OF MATHEMATİCS TEACHİNG İN A LEARNİNG ENVİRONMENT DESİGNED ACCORDİNG TO THE THEORY OF MULTİPLE INTELLİGENCES

    Directory of Open Access Journals (Sweden)

    Ramazan GÜRBÜZ

    2013-06-01

    Full Text Available The aim of this study is to evaluate qualitatively the themes emerging from the application of activities designed according to The Theory of Multiple Intelligences. The activities were selected from the learning domains of 7th grade mathematics curriculum. This case study was conducted with two mathematics teachers from different schools and their 54 students and it lasted total 51 hours. Interview and observation methods were used in order to collect data as well as student journals. After a content analysis of the interviews, codes and themes were derived with regard to the purpose of the study. Observations and student journals were presented by directly quoting the parts related with the themes derived from the interviews. As a result of this study, the activities carried out in environments designed according to The Theory of Multiple Intelligences were found to be effective in the context of the theme of "facilitating understanding and providing permanent learning". Since this kind of practices have the role of catalyzing in the the displacement process of learning environments based on alternative learning theories by traditional learning environments, the frequent implementation of similar practices in different subject fields and at different levels should be implemented for the widespread use of student centered approaches.

  13. THROUGH THE INTERVIEW TEXTS THE ANALYSIS OF THE 8TH GRADE STUDENTS' SKİLLS OF ASKİNG QUESTIONS ACCORDING TO THE REVISED BLOOM'S TAXONOMY

    OpenAIRE

    Yilmaz, Engin; KERAY, Betül

    2013-01-01

    This research has been made in order to determine how effective the education of asking questions is on students and in which stage the students achieve the acqusition “Forming questions about the text” which is stated in 8th grade students' Turkish Teaching Program. For this research, a school located in city centre in Aksaray was chosen, and one of the two classes- both of which have been taught by the same teacher- is selected conrol group and the the other is selected experiment group. Th...

  14. Experimental and theoretical analysis of effects of N2, O2 and Ar in excess air on combustion and NOx emissions of a turbocharged NG engine

    International Nuclear Information System (INIS)

    Highlights: • We defined 7 effects of excess air and quantitative indicators on NOx emissions. • Contributions of N2, O2 and Ar on the thermal effect are separately 85%, 14% and 1%. • Contributions of N2 and Ar on the dilution effect are 98.8% and 1.2%, respectively. • The high level of N2 in air is the main reason why NOx emissions decrease with λ. - Abstract: This paper presents the effects of N2, O2 and Ar in excess air on combustion and NOx emissions of a natural gas spark-ignition engine. Based on Arrhenius Law and Zeldovich Mechanism, seven different kinds of effects were defined as well as their quantitative indicators. Excess air and N2 were separately added into the intake charge with fuel remaining unchanged in a 6-cylinder turbocharged natural gas engine. The results show that the essential reason why NOx emissions vary with increasing excess air ratio (λ) is that the oxidation effect of O2 varies with the in-cylinder temperature, which is controlled by the thermal effect of excess air. The contributions of N2, O2 and Ar on the thermal effect are 82.6–86.1%, 13.3–16.7% and 0.6–0.7%, respectively. The chemical effect of O2 is fully offset by the dilution effect of N2 and Ar. The contributions of N2 and Ar on the dilution effect are 98.31% and 1.69%, respectively. The increase of NOx emissions caused by O2 is found to be increased first and then decreased with increasing λ. From the view of the composition of air, the high level of N2 is the main reason why NOx emissions decrease significantly with increasing λ

  15. Exploração de fatores de risco para câncer de mama em mulheres de etnia Kaingáng, Terra Indígena Faxinal, Paraná, Brasil, 2008 Exploring breast cancer risk factors in Kaingáng women in the Faxinal Indigenous Territory, Paraná State, Brazil, 2008

    Directory of Open Access Journals (Sweden)

    Edimara Patrícia da Silva

    2009-07-01

    Full Text Available O objetivo do presente estudo foi analisar a saúde das mulheres Kaingáng da Terra Indígena Faxinal, Paraná, Brasil, quanto aos fatores de risco para o câncer de mama. O estudo, de caráter descritivo-exploratório, foi realizado com 95,4% da população feminina com vinte anos ou mais. O aleitamento materno por mais de 1 ano foi relatado por 92,3% das mulheres; 41,4% tinham mais de cinco filhos; a idade média da menarca foi 13,4 anos; 61,5% nunca tinham ouvido falar do câncer de mama; o auto-exame da mama era realizado por apenas 2,9% das mulheres; o médico não realizava o exame clínico da mama, segundo 81,7% delas, e 99% nunca fizeram mamografia. Não foi relatado caso de câncer de mama ou extramamário. Destacaram-se a presença dos fatores de proteção para a carcinogênese e a ausência de alguns fatores de risco, como o uso de terapia de reposição hormonal por mais de cinco anos e etilismo, na maioria das mulheres Kaingáng.This study analyzes the health of Kaingáng women in the Faxinal indigenous territory in Paraná State, Brazil, with regard to risk factors for breast cancer. A descriptive and exploratory investigation was undertaken that included 95.4% of females 20 years or over. Data showed that 92.3% of Kaingáng women reported breastfeeding for more than one year; 41.4% had more than 5 children; mean age at menarche was 13.4 years; 61.5% had never heard of breast cancer; breast self-examination was only performed by 2.9%; 81.7% reported no clinical breast examination by a physician; 99% had never had a mammogram. No cases of breast or non-breast cancer were reported. The study highlights protective factors against carcinogenesis and the absence of key risk factors like hormone replacement therapy for more than five years and alcohol consumption in most Kaingáng women.

  16. NG-nitro-L-arginine methyl ester, an inhibitor of nitric oxide synthesis, ameliorates interleukin 2-induced capillary leakage and reduces tumour growth in adenocarcinoma-bearing mice.

    OpenAIRE

    Orucevic, A.; P. K. Lala

    1996-01-01

    We tested whether NG-nitro-L-arginine methyl ester (L-NAME), an inhibitor of nitric oxide (NO) synthesis, can prevent interleukin 2 (IL-2)-induced capillary leakage in tumour-bearing mice without compromising the therapeutic benefits of IL-2. C3H/HeJ female mice transplanted s.c. with 2.5 x 10(5) C3-L5 mammary carcinoma cells were treated with: nothing, IL-2 (ten injections of 15,000 Cetus units i.p. every 8 h), L-NAME (0.1, 0.5, or 1 mg ml-1 drinking water), IL-2 + L-NAME (0.1 or 0.5 or 1 mg...

  17. Characteristics of muscarinic receptors that selectively couple to inhibition of adenylate cyclase or stimulation of phospholipase C on NG108-15 and 1321N1 cells

    International Nuclear Information System (INIS)

    The purpose of this dissertation was to establish whether different muscarinic receptor proteins selectively couple to different second messenger response system. Although both second messenger response systems are fully functional in both cell lines, activation of muscarinic cholinergic receptors only results in inhibition of adenylate cyclase in NG108-15 neuroblastoma x glioma cells and stimulation of phosphoinositide hydrolysis in 1321N1 human astrocytoma cells. Muscarinic receptors on both cell types were covalently labeled with (3H)Propylbenzilylcholine mustard ((3H)PBCM) and the mobilities of the (3H)PBCM-labelled species of both cells were compared by SDS-PAGE. 1321N1 and NG108-15 cells each primarily expressed a single (3H)PBCM-labelled species with an apparent size of approximately 92,000 and 66,000 Da, respectively. (3H)PBCM labelling was completely inhibited by 1 μM atropine or by down-regulation of muscarinic receptors by an overnight incubation with carbachol. The apparent size of the (3H)PBCM-labelled species of both cell lines was not altered by treatment with a series of protease inhibitors or by treatment with dithiothreitol and iodoacetamide. Another approach for determining differences in the muscarinic receptors of 2 cells lines was to study agonist-induced alteration of muscarinic receptor number. Exposure of both cell types to agonists resulted in rapid loss of muscarinic receptors from cell surface without change of total cellular muscarinic receptors followed by subsequently loss of receptors from cells. Muscarinic receptors on both cell lines were regulated by agonist with similar properties

  18. Evaluation of the Bio-Rad Dx CT/NG/MG® assay for simultaneous detection of Chlamydia trachomatis, Neisseria gonorrhoeae and Mycoplasma genitalium in urine.

    Science.gov (United States)

    Ursi, D; Crucitti, T; Smet, H; Ieven, M

    2016-07-01

    We evaluated the performance of the Bio-Rad real-time Dx CT/NG/MG® assay for detection of C. trachomatis, N. gonorrhoeae and M. genitalium on a collection of 441 urine samples from sexually transmitted infections, or travellers consultations and from anonymous sperm donors that were previously analysed with the Abbott RealTime CT/NG assay. Samples positive for C. trachomatis or N. gonorrhoeae with the Abbott assay had all previously been confirmed with an in-house real-time PCR assay. Samples positive for M. genitalium with the Bio-Rad assay were subsequently analysed by an in-house real-time PCR. On a total of 441 urines, 104 samples were positive for C. trachomatis, 12 were positive for N. gonorrhoeae and seven were positive for M. genitalium. After retesting of discrepant results, the test results were completely concordant, resulting in a calculated sensitivity and specificity of the Bio-Rad assay of 98.1 % and 100 % for C. trachomatis and of 91.7 % and 100 % for N. gonorrhoeae. Results for M. genitalium with the Bio-Rad assay were also concordant with the results of an in house PCR. We also evaluated the performance of automated nucleic acid extractions of urine samples with the NucliSENS easyMAG (bioMérieux) compared to the manual DNA extraction prescribed by the insert of the kit. The easyMAG extraction gave lower Ct values, relieved inhibition and had a lower hands-on time. PMID:27116008

  19. δ-Opioid receptor-stimulated Akt signaling in neuroblastoma x glioma (NG108-15) hybrid cells involves receptor tyrosine kinase-mediated PI3K activation

    International Nuclear Information System (INIS)

    δ-Opioid receptor (DOR) agonists possess cytoprotective properties, an effect associated with activation of the 'pro-survival' kinase Akt. Here we delineate the signal transduction pathway by which opioids induce Akt activation in neuroblastoma x glioma (NG108-15) hybrid cells. Exposure of the cells to both [D-Pen2,5]enkephalin and etorphine resulted in a time- and dose-dependent increase in Akt activity, as measured by means of an activation-specific antibody recognizing phosphoserine-473. DOR-mediated Akt signaling is blocked by the opioid antagonist naloxone and involves inhibitory Gi/o proteins, because pre-treatment with pertussis toxin, but not over-expression of the Gq/11 scavengers EBP50 and GRK2-K220R, prevented this effect. Further studies with Wortmannin and LY294002 revealed that phophoinositol-3-kinase (PI3K) plays a central role in opioid-induced Akt activation. Opioids stimulate Akt activity through transactivation of receptor tyrosine kinases (RTK), because pre-treatment of the cells with inhibitors for neurotrophin receptor tyrosine kinases (AG879) and the insulin-like growth factor receptor IGF-1 (AG1024), but not over-expression of the Gβγ scavenger phosducin, abolished this effect. Activated Akt translocates to the nuclear membrane, where it promotes GSK3 phosphorylation and prevents caspase-3 cleavage, two key events mediating inhibition of cell apoptosis and enhancement of cell survival. Taken together, these results demonstrate that in NG108-15 hybrid cells DOR agonists possess cytoprotective properties mediated by activation of the RTK/PI3K/Akt signaling pathway.

  20. Mechanised flow system for on-line microwave digestion of food samples with off-line catalytic spectrophotometric determination of cobalt at ng l-1 levels.

    Science.gov (United States)

    Pereira-Filho, E R; Arruda, M A

    1999-12-01

    A mechanised system for on-line slurry food sample digestion was developed and an off-line cobalt determination was performed. The stabilised slurry sample was introduced into an air carrier stream until reaching the digestion coils located inside a household microwave oven. Software written in Visual Basic 3.0 was developed to permit the transport of the slurry samples and the programming of the microwave oven and also the control of the mineralization valve. The proposed system was optimized for determination of cobalt in certified samples such as mussels, bovine liver and fish and also uncertified fish samples. The digestion parameters were established as 3 mol l-1 HNO3 for mussels, 3 mol l-1 HNO3 plus 0.16% v/v H2O2 for bovine liver and 12 mol l-1 HNO3 for fish employing maximum power for 5 min of microwave actuation. In the subsequent spectrophotometric method for the catalytic determination of cobalt, the Tiron and hydrogen peroxide concentrations were 1.8 x 10(-3) and 3.0 x 10(-4) mol l-1, respectively, and the sample residence time was 300 s as determined by an optimisation process. The proposed method features a linear range from 10 to 200 ng l-1 Co (r > 0.996) with detection and quantification limits of 1.7 and 5.5 ng l-1 Co, respectively. The precision, expressed as RSD, was 2.4% (n = 12) for repeatability and 5.2% (n = 10) for reproducibility and the accuracy of the proposed method was assessed by using certified samples and an alternative technique (ETAAS). PMID:10746313

  1. {delta}-Opioid receptor-stimulated Akt signaling in neuroblastoma x glioma (NG108-15) hybrid cells involves receptor tyrosine kinase-mediated PI3K activation

    Energy Technology Data Exchange (ETDEWEB)

    Heiss, Anika; Ammer, Hermann [Institute of Pharmacology, Toxicology and Pharmacy Ludwig-Maximilians-University of Munich Koeniginstrasse 16 80539 Muenchen Federal Republic of Germany (Germany); Eisinger, Daniela A., E-mail: eisinger@pharmtox.vetmed.uni-muenchen.de [Institute of Pharmacology, Toxicology and Pharmacy Ludwig-Maximilians-University of Munich Koeniginstrasse 16 80539 Muenchen Federal Republic of Germany (Germany)

    2009-07-15

    {delta}-Opioid receptor (DOR) agonists possess cytoprotective properties, an effect associated with activation of the 'pro-survival' kinase Akt. Here we delineate the signal transduction pathway by which opioids induce Akt activation in neuroblastoma x glioma (NG108-15) hybrid cells. Exposure of the cells to both [D-Pen{sup 2,5}]enkephalin and etorphine resulted in a time- and dose-dependent increase in Akt activity, as measured by means of an activation-specific antibody recognizing phosphoserine-473. DOR-mediated Akt signaling is blocked by the opioid antagonist naloxone and involves inhibitory G{sub i/o} proteins, because pre-treatment with pertussis toxin, but not over-expression of the G{sub q/11} scavengers EBP50 and GRK2-K220R, prevented this effect. Further studies with Wortmannin and LY294002 revealed that phophoinositol-3-kinase (PI3K) plays a central role in opioid-induced Akt activation. Opioids stimulate Akt activity through transactivation of receptor tyrosine kinases (RTK), because pre-treatment of the cells with inhibitors for neurotrophin receptor tyrosine kinases (AG879) and the insulin-like growth factor receptor IGF-1 (AG1024), but not over-expression of the G{beta}{gamma} scavenger phosducin, abolished this effect. Activated Akt translocates to the nuclear membrane, where it promotes GSK3 phosphorylation and prevents caspase-3 cleavage, two key events mediating inhibition of cell apoptosis and enhancement of cell survival. Taken together, these results demonstrate that in NG108-15 hybrid cells DOR agonists possess cytoprotective properties mediated by activation of the RTK/PI3K/Akt signaling pathway.

  2. Andrew Ng 谈 Deep Learning

    Institute of Scientific and Technical Information of China (English)

    卢鸫翔

    2014-01-01

    Andrew Ng是Deep Learning领域最富盛名的科学家之一,他也是斯坦福人工智能实验室主任,在线教育平台Coursera的创始人之一。2014年5月,他加入百度,负责“百度大脑”计划,并担任百度首席科学家。目前,他接受了《程序员》的专访。

  3. Molecular detection and confirmation of Neisseria gonorrhoeae in urogenital and extragenital specimens using the Abbott CT/NG RealTime assay and an in-house assay targeting the porA pseudogene.

    LENUS (Irish Health Repository)

    Walsh, A

    2011-04-01

    Culture for detection of Neisseria gonorrhoeae (NG) is being replaced by molecular assays, but difficulties are observed with false positive and negatives results, especially for extragenital samples. This study evaluates the Abbott CT\\/NG Real-Time assay and a real-time porA pseudogene assay. Samples (n = 600) from a mixed prevalence Irish population include 164 male urines with corresponding urethral swabs, 58 endocervical swabs, 173 male pharyngeal swabs, 205 male rectal swabs, 36 NG clinical isolates and 26 commensal Neisseria species isolates. There was a 100% concordance between the Abbott CT\\/NG Real-Time and the porA assay. The positivity rate was 1.2%, 1.7%, 8.1% and 5.8% for FVU\\/urethral swabs, endocervical, pharyngeal and rectal swabs, respectively. These results were compared to culture and discrepancies were found with nine pharyngeal and three rectal swabs. Seven of the 12 discrepant positive samples were sequenced and were confirmed "true positives". The sensitivity and specificity of the molecular assays was 100%. The sensitivity of the culture-based testing was 100% for urogenital samples but 36% and 75% for pharyngeal and rectal swabs, respectively. The combined Abbott CT\\/NG and porA assays provide a valuable alternative to culture and also generate a significant increase in the diagnosis of pharyngeal and rectal NG infection.

  4. Prostate Health Index (Phi and Prostate Cancer Antigen 3 (PCA3 significantly improve prostate cancer detection at initial biopsy in a total PSA range of 2-10 ng/ml.

    Directory of Open Access Journals (Sweden)

    Matteo Ferro

    Full Text Available Many efforts to reduce prostate specific antigen (PSA overdiagnosis and overtreatment have been made. To this aim, Prostate Health Index (Phi and Prostate Cancer Antigen 3 (PCA3 have been proposed as new more specific biomarkers. We evaluated the ability of phi and PCA3 to identify prostate cancer (PCa at initial prostate biopsy in men with total PSA range of 2-10 ng/ml. The performance of phi and PCA3 were evaluated in 300 patients undergoing first prostate biopsy. ROC curve analyses tested the accuracy (AUC of phi and PCA3 in predicting PCa. Decision curve analyses (DCA were used to compare the clinical benefit of the two biomarkers. We found that the AUC value of phi (0.77 was comparable to those of %p2PSA (0.76 and PCA3 (0.73 with no significant differences in pairwise comparison (%p2PSA vs phi p = 0.673, %p2PSA vs. PCA3 p = 0.417 and phi vs. PCA3 p = 0.247. These three biomarkers significantly outperformed fPSA (AUC = 0.60, % fPSA (AUC = 0.62 and p2PSA (AUC = 0.63. At DCA, phi and PCA3 exhibited a very close net benefit profile until the threshold probability of 25%, then phi index showed higher net benefit than PCA3. Multivariable analysis showed that the addition of phi and PCA3 to the base multivariable model (age, PSA, %fPSA, DRE, prostate volume increased predictive accuracy, whereas no model improved single biomarker performance. Finally we showed that subjects with active surveillance (AS compatible cancer had significantly lower phi and PCA3 values (p<0.001 and p = 0.01, respectively. In conclusion, both phi and PCA3 comparably increase the accuracy in predicting the presence of PCa in total PSA range 2-10 ng/ml at initial biopsy, outperforming currently used %fPSA.

  5. Proteoglycan-based diversification of disease outcome in head and neck cancer patients identifies NG2/CSPG4 and syndecan-2 as unique relapse and overall survival predicting factors

    International Nuclear Information System (INIS)

    Tumour relapse is recognized to be the prime fatal burden in patients affected by head and neck squamous cell carcinoma (HNSCC), but no discrete molecular trait has yet been identified to make reliable early predictions of tumour recurrence. Expression of cell surface proteoglycans (PGs) is frequently altered in carcinomas and several of them are gradually emerging as key prognostic factors. A PG expression analysis at both mRNA and protein level, was pursued on primary lesions derived from 173 HNSCC patients from whom full clinical history and 2 years post-surgical follow-up was accessible. Gene and protein expression data were correlated with clinical traits and previously proposed tumour relapse markers to stratify high-risk patient subgroups. HNSCC lesions were indeed found to exhibit a widely aberrant PG expression pattern characterized by a variable expression of all PGs and a characteristic de novo transcription/translation of GPC2, GPC5 and NG2/CSPG4 respectively in 36%, 72% and 71% on 119 cases. Importantly, expression of NG2/CSPG4, on neoplastic cells and in the intralesional stroma (Hazard Ratio [HR], 6.76, p = 0.017) was strongly associated with loco-regional relapse, whereas stromal enrichment of SDC2 (HR, 7.652, p = 0.007) was independently tied to lymphnodal infiltration and disease-related death. Conversely, down-regulated SDC1 transcript (HR, 0.232, p = 0.013) uniquely correlated with formation of distant metastases. Altered expression of PGs significantly correlated with the above disease outcomes when either considered alone or in association with well-established predictors of poor prognosis (i.e. T classification, previous occurrence of precancerous lesions and lymphnodal metastasis). Combined alteration of all three PGs was found to be a reliable predictor of shorter survival. An unprecedented PG-based prognostic portrait is unveiled that incisively diversifies disease course in HNSCC patients beyond the currently known clinical and molecular

  6. Ang Paghiraya sa Nasyon: Ang mga Pagdiriwang ng Anibersaryo ng Komonwelt ng Pilipinas (1936-1941

    Directory of Open Access Journals (Sweden)

    Michael Charleston B. Chua

    2007-12-01

    Full Text Available The paper will revisit the forms and themes of commemoration of the anniversary of the Philippine Commonwealth every November 15 from 1936 to 1941. Similar to the present-day State of the Nation Address, the celebrations reflected the mood and sentiments of the government and served as the articulation of how the ruling elite democracy imagined the nation that would be established under the guidance of the United States of America. (1 The plans for national defense, (2 the loyalty of America, (3 the achievements of the Commonwealth government, (4 optimism for the future, (5 Pres. Manuel Quezon, the Great Leader, and (6 the excellence and capabilities of the Filipino, became the common themes of the celebrations reinforced yearly to rally the citizens to imagine the nation with the government in a ritualistic gathering.

  7. Post-pellet-digestion precipitation and solid phase extraction: A practical and efficient workflow to extract surrogate peptides for ultra-high performance liquid chromatography--tandem mass spectrometry bioanalysis of a therapeutic antibody in the low ng/mL range.

    Science.gov (United States)

    Gong, Chao; Zheng, Naiyu; Zeng, Jianing; Aubry, Anne-Françoise; Arnold, Mark E

    2015-12-11

    The current LC-MS/MS approach for bioanalysis of protein therapeutics requires generating peptides from protein molecules via trypsin digestion, followed by sensitive detection of these surrogate peptides by multiple reaction monitoring (MRM). However, the presence of huge amount of matrix-related interference peptides generated from trypsin digestion often causes substantial matrix effect or isobaric interferences during LC-MS/MS analysis. Solid phase extraction (SPE) exhibits great potential in sample extraction to overcome these challenges due to its characteristic features of high selectivity, reproducibility, cost-effectiveness and potential to be automated. Here, we report an effective SPE methodology for the bioanalysis of protein therapeutics involving post-pellet-digestion precipitation and SPE cleanup prior to UHPLC-MS/MS analysis. Specifically, proteins in serum samples were first precipitated with methanol to enrich the protein analyte in the pellet prior to trypsin digestion of the pellet (pellet-digestion). The trypsin digest was further processed by a post-pellet-digestion precipitation (second precipitation) to remove matrix-related clog-causing components prior to SPE on OASIS™ MAX (Mixed-Mode Anion-Exchange) SPE plate. This methodology successfully overcame SPE clogging issue and enabled extraction of 100μL of monkey serum on SPE with significant sensitivity improvement to achieve a lower limit of quantitation (LLOQ) of 50ng/mL for a human monoclonal antibody of the IgG4 subclass. This UHPLC-MS/MS assay was validated in a concentration range of 50-5000ng/mL with intra- and inter-assay precisions of within 9.6% CV, and assay accuracy of within ±2.9% Dev of their nominal concentrations. To our best knowledge, this is the pellet digestion with SPE method for LC-MS/MS bioanalysis of a monoclonal antibody for the first time to achieve a LLOQ in the low ng/mL concentration range. PMID:26525666

  8. Simultaneous recording of the action potential and its whole-cell associated ion current on NG108-15 cells cultured over a MWCNT electrode

    Science.gov (United States)

    Morales-Reyes, I.; Seseña-Rubfiaro, A.; Acosta-García, M. C.; Batina, N.; Godínez-Fernández, R.

    2016-08-01

    It is well known that, in excitable cells, the dynamics of the ion currents (I i) is extremely important to determine both the magnitude and time course of an action potential (A p). To observe these two processes simultaneously, we cultured NG108-15 cells over a multi-walled carbon nanotubes electrode (MWCNTe) surface and arranged a two independent Patch Clamp system configuration (Bi-Patch Clamp). The first system was used in the voltage or current clamp mode, using a glass micropipette as an electrode. The second system was modified to connect the MWCNTe to virtual ground. While the A p was recorded through the micropipette electrode, the MWCNTe was used to measure the underlying whole-cell current. This configuration allowed us to record both the membrane voltage (V m) and the current changes simultaneously. Images acquired by atomic force microscopy (AFM) and scanning electron microscopy (SEM) indicate that cultured cells developed a complex network of neurites, which served to establish the necessary close contact and strong adhesion to the MWCNTe surface. These features were a key factor to obtain the recording of the whole-cell I i with a high signal to noise ratio (SNR). The experimental results were satisfactorily reproduced by a theoretical model developed to simulate the proposed system. Besides the contribution to a better understanding of the fundamental mechanisms involved in cell communication, the developed method could be useful in cell physiology studies, pharmacology and diseases diagnosis.

  9. Marketingový plán pro letoun L410 NG společnosti Aircraft Industries, a.s.

    OpenAIRE

    Juráková, Hana

    2014-01-01

    Tato diplomová práce se zabývá sestavením marketingového plánu pro letoun L 410 NG, vyráběný firmou Aircraft Industries a.s. První část práce zahrnuje obecné teoretické poznatky, zaměřující se na proces sestavení plánu pomocí rozboru vnějšího okolí SLEPTE, Porterova modelu pěti konkurenčních sil, analýzy SWOT a marketingového mixu 5P. Ve druhé analytické části je tato základní teorie aplikována na uvedenou firmu. V závěrečné části práce jsou na základě zjištěných fakt a výsledků analýz navrhn...

  10. Incidental prostatic adenocarcinoma in patients with PSA less than 4 ng/ml undergoing radical cystoprostatectomy for bladder cancer in iranian men

    Directory of Open Access Journals (Sweden)

    S.Y. Hosseini

    2007-04-01

    Full Text Available OBJECTIVE:To assess the incidence of prostate adenocarcinoma in patients undergoing radical cystoprostatectomy due to bladder cancer in Iranian men. MATERIALS AND METHODS: Fifty cystoprostatectomy specimens removed due to bladder malignancy (2004-2005 at two referral centers (Shaheed Modarress and Shaheed Labbafinejad Hospitals, Tehran, Iran were examined for the coincidental finding of prostate cancer (PCa. At the time of surgery the patient's serum PSA was less than 4 ng/mL and there were no suspicious lesions by digital rectal examination. Pathologic grade, stage, morphometric volume, number of tumor foci and association with areas of high grade prostatic intraepithelial neoplasia (HGPIN were assessed by light microscopy. All specimens were totally embedded and whole-mounted. Clinically significant cancers were defined as tumors with 0.5 mL volume, Gleason pattern 4 or 5, pT3, positive surgical margin, and multifocality > 3. RESULTS: Incidentally detected cancer was found in 7 (14% of cystoprostatectomy specimens. HGPIN was present in 1 (14.3% of the cystoprostatectomies with incidentally detected prostate cancer. None of cystoprostatectomies without prostate cancer had HGPIN. Four (57% of the detected cancers were significant. CONCLUSION: We conclude that incidentally detected prostate cancer in Iran is lower than the rates reported in other countries. Further studies are warranted for better declaration of variability of prostate cancer between different ethnic groups.

  11. The transmission of symmetric 40 Gb/s TWDM-based NG-PON2 utilizing delay interferometer (DI) for RSOA bandwidth enhancement

    Science.gov (United States)

    Bindhaiq, Salem; Zulkifli, Nadiatulhuda; Supa'at, AbuSahmah M.

    2016-07-01

    Time and wavelength-division multiplexed passive optical network (TWDM-PON) has been finally selected as the pragmatic solution for the next-generation passive optical network stage 2 (NG-PON2). In this paper, we propose a symmetric 40 Gb/s TWDM-PON system with low cost reflective semiconductor optical amplifier (RSOA) for both downstream and upstream directions. A single bi-pass delay interferometer (DI), deployed in the optical line terminal (OLT), is used to enhance the poor performance of the RSOA with respect to the low bandwidth induced by laser chirp. With the help of the 40 GHz free spectrum range (FSR) DI, we show a successful transmission of the proposed work through simulation study where an aggregate capacity of 40 Gb/s is transported over 40 km transmission distance with 32 splits. The TWDM-PON system at BER of 10-6 has shown a minimum receiver sensitivity of -22.78 dBm and -22.71 dBm for both downstream and upstream, respectively with maximum power penalty of 2 dB for downstream channel and 2.39 dB for upstream channel.

  12. Influence of Zn on the oxide layer on AISI 316L(NG) stainless steel in simulated pressurised water reactor coolant

    International Nuclear Information System (INIS)

    The oxidation of AISI 316L(NG) stainless steel in simulated pressurised water reactor (PWR) coolant with or without addition of 1 ppm Zn at 280 deg. C for up to 96 h has been characterised in situ by electrochemical impedance spectroscopy (EIS), both at the corrosion potential and under anodic polarisation up to 0.5 V vs. the reversible hydrogen electrode (RHE). Additional tests were performed in simulated PWR coolant with the addition of 0.01 M Na2B4O7 to exclude the effect of pH excursions probably due to Zn hydrolysis reactions. The thickness and in-depth composition of the oxide films formed at open circuit and at 0.5 V vs. RHE in the investigated electrolytes have been estimated from X-ray photoelectron spectroscopy (XPS) depth profiles. The kinetic and transport parameters characterising the oxide layer growth have been estimated using a calculational procedure based on the mixed conduction model for oxide films. Successful simulations of both the EIS and XPS data have been obtained. The parameter estimates are discussed in terms of the effect of Zn on the oxide layers on stainless steel in PWR conditions

  13. Analysis of vesicle fluid following the sting of the lionfish Pterois volitans.

    Science.gov (United States)

    Auerbach, P S; McKinney, H E; Rees, R S; Heggers, J P

    1987-01-01

    Fluid aspirated from blisters following a lionfish (Pterois volitans) sting was analyzed utilizing combined capillary column gas chromatography and negative ion chemical ionization mass spectrometry. Analysis for prostaglandin F2 alpha demonstrated 16.91 ng/ml, for prostaglandin E2 0.143 ng/ml, for 6-keto-prostaglandin F1 alpha less than 0.1 ng/ml (nondetectable) and for thromboxane B2 1.65 ng/ml. Platelet aggregation studies showed that blister fluid caused aggregation of isolated platelets only, which was inhibited by heat treatment or by the presence of normal donor plasma. PMID:3438924

  14. Protection of panaxnotoginsengsaponins against NG108-15 cells in senile dementia model induced by amyoid beta-peptide 25- 35%三七总皂甙对淀粉样β25~35肽诱导的NG108-15细胞老年性痴呆模型的保护作用

    Institute of Scientific and Technical Information of China (English)

    卢忠朋; 王乃平; 钟振国

    2004-01-01

    背景:阿尔茨海默病(Alzheimer disease,AD)的病理病因虽然还没有完全清楚,但目前认为β-淀粉样肽(amyloid β-peptide,Aβ)是引起AD的胆碱能神经功能紊乱的主要成分.因此,Aβ的产生及其对神经细胞的影响,以及寻找该病的发病机制、治疗方法及其有效的治疗药物成了医药界的研究热点.目的:观察三七总皂甙(PNS)对NG108-15细胞老年性痴呆模型的影响,为寻找该病的发病机制提供佐证,为研究和开发中药的新成分提供理论依据.设计:体外平行对照实验.单位:广西中医学院新药开发中心,广西中医学院生物化学教研室.对象:实验在广西中医学院新药开发中心完成.NG108-15细胞:中国科学院上海细胞研究所提供.PNS:云南玉溪维和制药厂生产.Aβ25~35片段:德国Sigma公司产品,批号:042K49501.MTT(四甲基噻唑蓝),德国Sigma公司产品,批号:020609.cAMP(环一磷酸腺苷)日本ヤマサ酱油株式会社产品,批号:00207.胚胎牛血清(FBS):由美国Hyclone公司提供,批号:0405.DMEM培养基:美国GIBCO公司产品,批号:0311.干预:NG108-15细胞培养法:含50 mL/L胚牛血清、10 g/L HAT、10 g/L青霉素和链霉素(1:1)的DMEM培养液,置100 mL/L CO2、37℃用的培养箱内培养殖,每隔一天换一次液,4 d传代一次.主要观察指标:利用MTT法、细胞计数法和细胞形态学检测法观察PNS对NG108-15细胞存活率和细胞突起的改变.结果:PNS能增加Aβ25~35片段神经毒性诱导的NG108-15细胞存活率[增殖状态:(311±21)%,分化状态:(113±31)%,P<0.05,F=5.567]、细胞突起率[增殖状态:(25.66±3.65)%,分化状态:(67.97±2.47)%,P<0.05,F=6.471]和细胞突起平均长度[增殖状态:(25.66±3.09)μm,分化状态:(25.66±3.65)μm,P<0.001,F=12.641].结论:PNS具有减轻细胞对Aβ的神经毒性反应和促进细胞突起生长的作用,表明PNS能对老年性痴呆的病理发展有拮抗作用.%BACKGROUND: Although the pathology and

  15. Contribuição da densidade do PSA para predizer o câncer da próstata em pacientes com valores de PSA entre 2,6 e 10,0 ng/ml

    Directory of Open Access Journals (Sweden)

    Hugo Alexandre Sócrates de Castro

    2011-08-01

    Full Text Available OBJETIVO: Estudar o perfil dos pacientes submetidos a biópsia prostática, determinando possíveis padrões que, associados aos níveis de PSA entre 2,6 e 10,0 ng/ml, possam levar a uma diminuição de biópsias desnecessárias. MATERIAIS E MÉTODOS: De 2007 a 2009, foi realizado um estudo transversal com 1.282 indivíduos submetidos a biópsia prostática e que apresentavam níveis de PSA entre 2,6 e 10,0 ng/ml. RESULTADOS: A prevalência de câncer foi de 28,6%. Pacientes com câncer eram, em média, mais idosos, com valores de PSA e densidade de PSA mais altos e menor volume da próstata. Na análise da densidade de PSA, os pacientes com câncer tiveram média de 0,31 ng/ml/cc, enquanto nos pacientes com resultado negativo a média foi de 0,10 ng/ml/cc. Utilizando como critério de positividade para câncer o ponto de corte de densidade de PSA de 0,15 ng/ml/cc, obtivemos especificidade de 74% e sensibilidade de 70%. Para aumentar a sensibilidade é preciso reduzir o ponto de corte. Com o valor 0,09 ng/ml/cc, obtivemos sensibilidade de 84% (IC 95%: 80-87% e especificidade de 75% (IC 95%: 72-78%. CONCLUSÃO: O uso sistemático da densidade de PSA na indicação de prosseguimento da investigação do paciente com biópsia poderia reduzir a quantidade de procedimentos desnecessários.

  16. Anthraquinones and nitrogen secondary metabolites from the coral-derived symbiotic fungi NG-15 -3 of South China Sea%南海珊瑚共附生真菌 NG -15-3中蒽醌和含氮类次生代谢产物研究

    Institute of Scientific and Technical Information of China (English)

    李秀婷; 刘炳新; 胡谷平; 汪玉梅; 张翠仙

    2016-01-01

    In order to study the secondary metabolites from the coral-derived symbiotic fungi NG-15 -3 of South China Sea,the compounds were isolated by silica gel column chromatography and HPLC.Their structures were identified on the basis of spectral data and physical constant comparison.Four anthraqui-nones and six nitrogen compounds were isolated as preechinulin (1),emodin (2),1-O -methyl emodin (3),physcion (4),carviolin (5),cyclo-(Phe-Gly)(6),cyclo-(Gly-Pro)(7),3H-imidazole-4-carboxylic acid (8),2-methyl-3H-imidazole-4-carboxylic acid (9)and 1H-pyrrole-2-carboxylic acid (10).All the secondary metabolites were firstly acquired from the coral-derived symbiotic fungi NG-15 -3 of South China Sea and compound 1 was a precursors of the monodehydro -2,5-diketopiperazines, which showed varied biological activity.%为了研究南海珊瑚共附生真菌 NG -15-3次生代谢产物,采用硅胶柱层析和 HPLC 等分离手段从乙酸乙酯提取部位分离得到单体化合物,结合现代波谱技术、物理常数对照对单体化合物的结构进行结构鉴定。从其分离得到4个蒽醌类:emodin (2)、1-O -methyl emodin (3)、physcion (4)、carviolin (5)和6个含氮类次生代谢产物:preechinulin (1)、cyclo-(Phe-Gly)(6)、cyclo-(Gly-Pro)(7)、3H-imidazole-4-carboxylic acid (8)、2-methyl-3H-imidazole-4-carboxylic acid (9)和1H-pyrrole-2-carboxylic acid (10)。所有化合物均为首次从该真菌中分离得到,且化合物1为单去氢2,5-二酮哌嗪类化合物的前体物质,具有丰富的生理活性。

  17. Efficiency of 6- and 12-punctures biopsies to detect prostate cancer in patients with PSA< 10 ng/mL and normal digital rectal examination

    Directory of Open Access Journals (Sweden)

    Luiz E. Slongo

    2003-02-01

    Full Text Available OBJECTIVE: Establish the efficiency of 6- and 12-punctures transrectal ultrasound-guided needle biopsies in low risk patients for prostate cancer. Six-punctures (sextant biopsies were compared to 12-punctures biopsies, assessing which is the best strategy to detect this neoplasm. MATERIALS AND METHODS: Among 240 patients submitted to prostate biopsy, 54 with suspected small and organ-localized tumors (prostatic specific antigen < 10 ng/mL and digital exam of the prostate not suggesting cancer in glands < 50 cm³ were selected, constituting a homogenous sample. These patients were submitted to standard 3-punctures (basal, mid, and apical sextant biopsy in parasagittal midline of each prostatic lobe, with 3 additional lateral punctures, bilaterally. Each specimen was separately submitted to histological study. RESULTS: Twenty-two (40.7% patients had prostatic cancer, and 28 presented prostatic hyperplasia, associated or not to inflammatory conditions. High-grade prostatic intraepithelial neoplasia (PIN was detected in 4 patients. From 22 tumors detected by 12-punctures biopsies, 6-punctures biopsies in the parasagittal midline (sextant diagnosed 50% of the cases, while isolated lateral punctures diagnosed 90.9% of the malignant neoplasms. Basal lateral punctures responded for 72.7% of the cancer diagnosis, while basal sextant punctures responded only for 9.1% of the cases. CONCLUSION: For low risk prostate cancer, patients’ 12-punctures biopsy was more effective, for sextant biopsy failed to diagnose half of the cases of neoplasm. Three lateral punctures (basal, mid, and apical, with 2 additional punctures in the parasagittal midline (mid and apical bilaterally are suggested as the best biopsy strategy.

  18. A novel analytical system involving hydride generation and gold-coated W-coil trapping atomic absorption spectrometry for selenium determination at ng l-1 level

    International Nuclear Information System (INIS)

    A novel analytical technique was developed where gaseous hydrogen selenide formed by sodium tetrahydroborate reduction is transported to and trapped on a resistively heated gold-coated W-coil atom trap for in situ preconcentration. Gold coating on W-coil was prepared by using an organic solution of Au. The atom trap is held at 165 oC during the collection stage and is heated up to 675 deg. C for revolatilization; analyte species formed are transported to an externally heated quartz T-tube where the atomization takes place and the transient signal is obtained. The carrier gas consisted of 112.5 ml min-1 Ar with 75 ml min-1 H2 during the collection step and 112.5 ml min-1 Ar with 450 ml min-1 H2 in the revolatilization step. The half width of the transient signal obtained is less than 0.5 s. The RSD for the measurements was found to be 3.9% (n = 11) for 0.10 μg l-1 Se using peak height measurements. The calibration plot for 27.0 ml of sample collected in 4.0 min using a flow rate of 6.75 ml min-1 was linear between 0.13 and 2.0 μg l-1 of Se. The limit of detection (3 s) is 39 ng l-1. The enhancement factor for the characteristic concentration (Co) was found to be 20.1 when compared to conventional hydride generation atomic absorption spectrometry system without trap. In order to check the accuracy of the method, standard reference material, natural water NIST 1640 was employed; the result was found to be in good agreement with the certified value at the 95% confidence level

  19. Activity-based protein profiling of secreted cellulolytic enzyme activity dynamics in Trichoderma reesei QM6a, NG14, and RUT-C30

    Energy Technology Data Exchange (ETDEWEB)

    Anderson, Lindsey N.; Culley, David E.; Hofstad, Beth A.; Chauvigne-Hines, Lacie M.; Zink, Erika M.; Purvine, Samuel O.; Smith, Richard D.; Callister, Stephen J.; Magnuson, Jon M.; Wright, Aaron T.

    2013-12-01

    Development of alternative, non-petroleum based sources of bioenergy that can be applied in the short-term find great promise in the use of highly abundant and renewable lignocellulosic plant biomass.1 This material obtained from different feedstocks, such as forest litter or agricultural residues, can yield liquid fuels and other chemical products through biorefinery processes.2 Biofuels are obtained from lignocellulosic materials by chemical pretreatment of the biomass, followed by enzymatic decomposition of cellulosic and hemicellulosic compounds into soluble sugars that are converted to desired chemical products via microbial metabolism and fermentation.3, 4 To release soluble sugars from polymeric cellulose multiple enzymes are required, including endoglucanase, exoglucanase, and β-glucosidase.5, 6 However, the enzymatic hydrolysis of cellulose into soluble sugars remains a significant limiting factor to the efficient and economically viable utilization of lignocellulosic biomass for transport fuels.7, 8 The primary industrial source of cellulose and hemicellulases is the mesophilic soft-rot fungus Trichoderma reesei,9 having widespread applications in food, feed, textile, pulp, and paper industries.10 The genome encodes 200 glycoside hydrolases, including 10 cellulolytic and 16 hemicellulolytic enzymes.11 The hypercellulolytic catabolite derepressed strain RUT-C30 was obtained through a three-step UV and chemical mutagenesis of the original T. reesei strain QM6a,12, 13 in which strains M7 and NG14 were intermediate, having higher cellulolytic activity than the parent strain but less activity and higher catabolite repression than RUT-C30.14 Numerous methods have been employed to optimize the secreted enzyme cocktail of T. reesei including cultivation conditions, operational parameters, and mutagenesis.3 However, creating an optimal and economical enzyme mixture for production-scale biofuels synthesis may take thousands of experiments to identify.

  20. Effects of Nitric Oxide Production Inhibitor Named, NG-Nitro-L-Arginine Methyl Ester (L-NAME, on Rat Mesenchymal Stem Cells Differentiation

    Directory of Open Access Journals (Sweden)

    E Arfaei

    2010-04-01

    Full Text Available Introduction & Objectives: Recently, the findings of some studies have shown that, nitric oxide (NO probably has an important role in differentiation of mesenchymal stem cells to osteoblasts. The aim of the present investigation was to study the effects of nitric oxide production inhibitor named, NG-nitro-L-arginine methyl ester (L-NAME, on rat mesenchymal stem cells differentiation to osteoblasts in vitro. Materials & Methods: This was an experimental study conducted at Hamedan University of Medical Sciences in 2009, in which rat bone marrow stem cells were isolated in an aseptic condition and cultured in vitro. After third passage, the cells were cultured in osteogenic differentiation medium. To study the effects of L-NAME on osteogenic differentiation, the L-NAME was added to the culture medium at a concentration of 125, 250, and 500 μM in some culture plates. During the culture procedure, the media were replaced with fresh ones, with a three days interval. After 28 days of culturing the mineralized matrix was stained using Alizarian red staining method. The gathered data were analyzed by SPSS software version 12 using one way ANOVA. Results: The findings of this study showed that in the presence of L-NAME, differentiation of bone marrow mesenchymal stem cells to osteoblasts was disordered and matrix mineralization significantly decreased in a dose dependent manner. Conclusion: This study revealed that, inhibition of nitric oxide production using L-NAME can prevent the differentiation of rat bone marrow mesenchymal stem cells to osteoblast. The results imply that NO is an important constituent in differentiation of mesenchymal stem cell to osteoblasts.

  1. Perfil nutricional e fatores associados à ocorrência de desnutrição entre crianças indígenas Kaingáng da Terra Indígena de Mangueirinha, Paraná, Brasil Nutritional profile and factors associated with malnutrition in Kaingáng children on the Mangueirinha Indigenous Reserve, Paraná State, Brazil

    Directory of Open Access Journals (Sweden)

    Adriana Masiero Kühl

    2009-02-01

    Full Text Available Este estudo objetivou avaliar o estado nutricional de crianças indígenas e verificar os fatores associados à ocorrência de agravos nutricionais. Foram avaliadas 141 crianças Kaingáng de 0 a 60 meses de idade da Terra Indígena de Mangueirinha, Paraná, Brasil, por meio das medidas de peso e altura ou comprimento. Realizaram-se entrevistas com a aplicação de um questionário sobre condições materno-infantis e sócioeconômicas. Segundo os parâmetros da Organização Mundial da Saúde (2006, 24,8% das crianças apresentavam déficit de altura para idade (AI, 9,2% baixo peso para idade (PI, 2,1% baixo peso para altura (PA e 2,1% baixo peso segundo o índice de massa corporal para idade (IMCI. De acordo com o NCHS (1977, 19,9% das crianças apresentaram baixa AI, 9,2% baixo PI e 1,4% baixo PA. O sobrepeso chegou a 6,4%, segundo o IMCI. O baixo peso ao nascer e os materiais utilizados na construção das paredes das casas estiveram estatisticamente associados com déficits antropométricos. Este estudo mostra que as crianças Kaingáng estão inseridas em um contexto marcado por precárias condições de vida, que estão associadas com indicadores desfavoráveis do estado nutricional.The goal of this study was to evaluate the nutritional status of indigenous children and to investigate factors associated with nutritional deficits. Weight and height measurements were obtained for 141 Kaingáng children from 0 to 5 years of age living on the Mangueirinha Indigenous Reserve in Paraná State, Brazil. Data on maternal and infant conditions and socioeconomic characteristics were also gathered through face-to-face interviews. Based on World Health Organization criteria (2006, 24.8% of the children presented low height-for-age (HA, 9.2% low weight-for-age (WA, 2.1% low weight-for-height (WH, and 2.1% low weight according to body mass index for age (BMIA. Based on NCHS criteria (1977, 19.9% of the children presented low HA, 9.2% low WA, and 1

  2. Perfil nutricional e fatores associados à ocorrência de desnutrição entre crianças indígenas Kaingáng da Terra Indígena de Mangueirinha, Paraná, Brasil Nutritional profile and factors associated with malnutrition in Kaingáng children on the Mangueirinha Indigenous Reserve, Paraná State, Brazil

    OpenAIRE

    Adriana Masiero Kühl; Arlete Catarina Tittoni Corso; Maurício Soares Leite; João Luiz Bastos

    2009-01-01

    Este estudo objetivou avaliar o estado nutricional de crianças indígenas e verificar os fatores associados à ocorrência de agravos nutricionais. Foram avaliadas 141 crianças Kaingáng de 0 a 60 meses de idade da Terra Indígena de Mangueirinha, Paraná, Brasil, por meio das medidas de peso e altura ou comprimento. Realizaram-se entrevistas com a aplicação de um questionário sobre condições materno-infantis e sócioeconômicas. Segundo os parâmetros da Organização Mundial da Saúde (2006), 24,8% das...

  3. Organização e qualidade da assistência à saúde dos índios Kaingáng do Rio Grande do Sul, Brasil Organization and quality of health care for Kaingáng Indians in Rio Grande do Sul, Brazil

    Directory of Open Access Journals (Sweden)

    Yara Hahr Marques Hökerberg

    2001-03-01

    Full Text Available Este estudo pretende avaliar o atendimento à saúde prestado aos índios Kaingáng do Rio Grande do Sul. Os óbitos evitáveis por assistência básica de saúde dos indígenas, ocorridos entre 1985 e 1995, foram comparados aos óbitos do Estado do Rio Grande do Sul. Os dados secundários relativos aos serviços de saúde foram complementados por entrevistas realizadas em campo com lideranças indígenas e com funcionários das instituições envolvidas na assistência à saúde do índio. Foi utilizado o Sistema de Informações Geográficas, visando relacionar a distribuição dos óbitos ao grau de acesso aos serviços de saúde. As áreas indígenas Kaingáng estão ligadas por estradas pavimentadas a municípios que contam com, pelo menos, um centro de saúde, ou ainda, hospitais de pequeno porte. O segundo nível de atenção situa-se em Palmeiras das Missões e Frederico Westphallen e o terceiro nível, em Passo Fundo. O que diferencia estas áreas indígenas do restante do Estado do Rio Grande do Sul é a quantidade de óbitos evitáveis pela assistência básica de saúde, tais como as causas mal definidas, a desnutrição, a tuberculose e o câncer cérvico-uterino.This study assesses the health care provided to Kaingáng Indians in Rio Grande do Sul, Brazil. Deaths preventable by primary health care among the Indians and occurring from 1985 to 1995 were compared to the same rates for the State of Rio Grande do Sul as a whole. Secondary data on health care services were supplemented by field interviews with indigenous leaders and with employees from participating institutions. A Geographic Information System (GIS was used to correlate distribution of deaths and access to health services. The Kaingáng settlements are connected by paved roads to counties with at least a public health clinic or even a small hospital in some cases. Secondary referrals are treated in Palmeiras das Missões and Frederico Westphallen and tertiary care is

  4. pg-ng级Os同位素热表面电离质谱高精度分析测试技术%High Precise Isotopic Measurements of pg-ng Os by Negative Ion Thermal Ionization Mass Spectrometry

    Institute of Scientific and Technical Information of China (English)

    李超; 杨雪; 赵鸿; 周利敏; 杜安道; 李欣尉; 屈文俊

    2015-01-01

    随着Os同位素在地质学领域定年与示踪的广泛应用,对分析测试精度和检出限提出了更高的要求。本文利用热表面电离质谱负离子模式( HTIMS)对OsO3-进行测定,通过试剂纯化、器皿清洗等前处理步骤的优化保证了超低的化学流程空白和获得灵敏而稳定的仪器测量信号,建立了高精度pg-ng级Os同位素分析测试方法。采用逐级剥谱法扣除氧同位素干扰,采用内标迭代法按照指数规律对仪器的质量歧视效应进行校正,全流程Os空白为0.41 pg,1 pg的190 Os计数约85000 cps,质量分馏系数在0.1%以内。pg级Os采用离子计数器动态跳峰扫描模式进行测量,187 Os/188 Os值测量精度小于在0.2%以内;ng级Os采用法拉第杯静态接收模式采集信号,187 Os/188 Os值测量精度在0.005%以内。经国际上Os同位素标准溶液H2、JMC以及国家一级Re-Os同位素标准物质JCBY的验证,分析精度达到了国际同类实验室先进水平。本方法具有低空白、高灵敏度、高精度的特点,可为地质学研究提供高精度Os同位素数据。%Higher analytical precision and lower detection limit are required in geological dating and tracing,due to the wide application of Os. In this study,high-precision determination of pg-ng Os by Hegative Ion Thermal Ionization Mass Spectrometry( HTIMS ) was established by reagent purification,vessel cleaning and chemical preparation to ensure the ultra-low blank and obtain sensitive and stable signals. Isobaric oxygen interferences are corrected by stepwise spectrum stripping,and mass discrimination effect is corrected by internal standard iteration. The total procedural blank of Os is 0. 41 pg,the signal of 1 pg 190 Os is about 85000 cps,and the mass fractionation factor is less than 0 . 1%. Pg level of Os is measured by ion counter dynamic jumping scan mode and the measurement precision of 187 Os/ 188 Os is less than 0 . 2%. ng level of Os

  5. Effect of electric acupuncture on the expression of NgR in the cerebral cortex,the medulla oblongata,and the spinal cord of hypertensive rats after cerebral infarction

    Institute of Scientific and Technical Information of China (English)

    谭峰

    2014-01-01

    Objective To observe the effect of electric acupuncture(EA)on the Nogo receptors(NgR)protein expression in the cerebral cortex,the medulla oblongata,and the spinal cord of cerebral ischemia-reperfusion(I/R)stroke-prone renovascular hypertensive rats(RHRSP)with middle cerebral artery occlusion(MCAO)at different time points,and to investigate its possible mecha-

  6. Lead determination at ng/mL level by flame atomic absorption spectrometry using a tantalum coated slotted quartz tube atom trap.

    Science.gov (United States)

    Demirtaş, İlknur; Bakırdere, Sezgin; Ataman, O Yavuz

    2015-06-01

    Flame atomic absorption spectrometry (FAAS) still keeps its importance despite the relatively low sensitivity; because it is a simple and economical technique for determination of metals. In recent years, atom traps have been developed to increase the sensitivity of FAAS. Although the detection limit of FAAS is only at the level of µg/mL, with the use of atom traps it can reach to ng/mL. Slotted quartz tube (SQT) is one of the atom traps used to improve sensitivity. In atom trapping mode of SQT, analyte is trapped on-line in SQT for few minutes using ordinary sample aspiration, followed by the introduction of a small volume of organic solvent to effect the revolatilization and atomization of analyte species resulting in a transient signal. This system is economical, commercially available and easy to use. In this study, a sensitive analytical method was developed for the determination of lead with the help of SQT atom trapping flame atomization (SQT-AT-FAAS). 574 Fold sensitivity enhancement was obtained at a sample suction rate of 3.9 mL/min for 5.0 min trapping period with respect to FAAS. Organic solvent was selected as 40 µL of methyl isobutyl ketone (MIBK). To obtain a further sensitivity enhancement inner surface of SQT was coated with several transition metals. The best sensitivity enhancement, 1650 fold enhancement, was obtained by the Ta-coated SQT-AT-FAAS. In addition, chemical nature of Pb species trapped on quartz and Ta surface, and the chemical nature of Ta on quartz surface were investigated by X-ray photoelectron spectroscopy (XPS) and Raman Spectroscopy. Raman spectrometric results indicate that tantalum is coated on SQT surface in the form of Ta2O5. XPS studies revealed that the oxidation state of Pb in species trapped on both bare and Ta coated SQT surfaces is +2. For the accuracy check, the analyses of standard reference material were performed by use of SCP SCIENCE EnviroMAT Low (EU-L-2) and results for Pb were to be in good agreement with

  7. Dynamic contrast enhanced MRI detects early response to adoptive NK cellular immunotherapy targeting the NG2 proteoglycan in a rat model of glioblastoma.

    Directory of Open Access Journals (Sweden)

    Cecilie Brekke Rygh

    Full Text Available There are currently no established radiological parameters that predict response to immunotherapy. We hypothesised that multiparametric, longitudinal magnetic resonance imaging (MRI of physiological parameters and pharmacokinetic models might detect early biological responses to immunotherapy for glioblastoma targeting NG2/CSPG4 with mAb9.2.27 combined with natural killer (NK cells. Contrast enhanced conventional T1-weighted MRI at 7±1 and 17±2 days post-treatment failed to detect differences in tumour size between the treatment groups, whereas, follow-up scans at 3 months demonstrated diminished signal intensity and tumour volume in the surviving NK+mAb9.2.27 treated animals. Notably, interstitial volume fraction (ve, was significantly increased in the NK+mAb9.2.27 combination therapy group compared mAb9.2.27 and NK cell monotherapy groups (p = 0.002 and p = 0.017 respectively in cohort 1 animals treated with 1 million NK cells. ve was reproducibly increased in the combination NK+mAb9.2.27 compared to NK cell monotherapy in cohort 2 treated with increased dose of 2 million NK cells (p<0.0001, indicating greater cell death induced by NK+mAb9.2.27 treatment. The interstitial volume fraction in the NK monotherapy group was significantly reduced compared to mAb9.2.27 monotherapy (p<0.0001 and untreated controls (p = 0.014 in the cohort 2 animals. NK cells in monotherapy were unable to kill the U87MG cells that highly expressed class I human leucocyte antigens, and diminished stress ligands for activating receptors. A significant association between apparent diffusion coefficient (ADC of water and ve in combination NK+mAb9.2.27 and NK monotherapy treated tumours was evident, where increased ADC corresponded to reduced ve in both cases. Collectively, these data support histological measures at end-stage demonstrating diminished tumour cell proliferation and pronounced apoptosis in the NK+mAb9.2.27 treated tumours compared to the other

  8. Dynamic Contrast Enhanced MRI Detects Early Response to Adoptive NK Cellular Immunotherapy Targeting the NG2 Proteoglycan in a Rat Model of Glioblastoma

    Science.gov (United States)

    Thuen, Marte; Gras Navarro, Andrea; Huuse, Else Marie; Thorsen, Frits; Poli, Aurelie; Zimmer, Jacques; Haraldseth, Olav; Lie, Stein Atle; Enger, Per Øyvind; Chekenya, Martha

    2014-01-01

    There are currently no established radiological parameters that predict response to immunotherapy. We hypothesised that multiparametric, longitudinal magnetic resonance imaging (MRI) of physiological parameters and pharmacokinetic models might detect early biological responses to immunotherapy for glioblastoma targeting NG2/CSPG4 with mAb9.2.27 combined with natural killer (NK) cells. Contrast enhanced conventional T1-weighted MRI at 7±1 and 17±2 days post-treatment failed to detect differences in tumour size between the treatment groups, whereas, follow-up scans at 3 months demonstrated diminished signal intensity and tumour volume in the surviving NK+mAb9.2.27 treated animals. Notably, interstitial volume fraction (ve), was significantly increased in the NK+mAb9.2.27 combination therapy group compared mAb9.2.27 and NK cell monotherapy groups (p = 0.002 and p = 0.017 respectively) in cohort 1 animals treated with 1 million NK cells. ve was reproducibly increased in the combination NK+mAb9.2.27 compared to NK cell monotherapy in cohort 2 treated with increased dose of 2 million NK cells (p<0.0001), indicating greater cell death induced by NK+mAb9.2.27 treatment. The interstitial volume fraction in the NK monotherapy group was significantly reduced compared to mAb9.2.27 monotherapy (p<0.0001) and untreated controls (p = 0.014) in the cohort 2 animals. NK cells in monotherapy were unable to kill the U87MG cells that highly expressed class I human leucocyte antigens, and diminished stress ligands for activating receptors. A significant association between apparent diffusion coefficient (ADC) of water and ve in combination NK+mAb9.2.27 and NK monotherapy treated tumours was evident, where increased ADC corresponded to reduced ve in both cases. Collectively, these data support histological measures at end-stage demonstrating diminished tumour cell proliferation and pronounced apoptosis in the NK+mAb9.2.27 treated tumours compared to the other groups. In

  9. NG-nitro-L-arginine methyl ester, an inhibitor of nitric oxide synthesis, ameliorates interleukin 2-induced capillary leakage and reduces tumour growth in adenocarcinoma-bearing mice.

    Science.gov (United States)

    Orucevic, A.; Lala, P. K.

    1996-01-01

    We tested whether NG-nitro-L-arginine methyl ester (L-NAME), an inhibitor of nitric oxide (NO) synthesis, can prevent interleukin 2 (IL-2)-induced capillary leakage in tumour-bearing mice without compromising the therapeutic benefits of IL-2. C3H/HeJ female mice transplanted s.c. with 2.5 x 10(5) C3-L5 mammary carcinoma cells were treated with: nothing, IL-2 (ten injections of 15,000 Cetus units i.p. every 8 h), L-NAME (0.1, 0.5, or 1 mg ml-1 drinking water), IL-2 + L-NAME (0.1 or 0.5 or 1 mg ml-1 drinking water). Therapies were given in one round (IL-2, days 10-13; L-NAME, days 9-13) or in two rounds (IL-2, days 10-13 and 20-23; L-NAME, days 9-13 and days 19-23) after tumour transplantation. Capillary leakage was measured from the water contents of the pleural cavities, lungs, spleen and kidneys. Effects of the therapies on the primary tumour size and the number of spontaneous lung metastases were also recorded. NO production was measured as the nitrite + nitrate levels in the serum and in the pleural effusion. After the first round of therapies, addition of L-NAME significantly reduced IL-2-induced pulmonary oedema and water retention in the spleen in a dose-dependent manner. It also significantly reduced the IL-2-induced rise in NO levels in the serum and pleural fluid, but did not affect IL-2-induced pleural effusion or water retention in the kidney. At later stages of tumour growth (day 23), tumours themselves induced significant fluid retention in the lungs and the kidney, which was not aggravated further with the second round of IL-2 therapy. At this time, L-NAME therapy alone ameliorated tumour-induced pulmonary oedema. During both rounds of therapy different doses of L-NAME alone caused a reduction of primary tumour growth as well as spontaneous lung metastases, which improved further with the addition of IL-2. The combination therapy was at least as effective as IL-2 therapy. In summary, L-NAME had anti-tumour effects in vivo, reduced the severity of IL-2

  10. Effect of a nitric oxide synthase inhibitor NG-nitro-L-arginine methyl ester on invasion of human colorectal cancer cell line SL-174T

    Institute of Scientific and Technical Information of China (English)

    Li-Bo Yu; Xin-Shu Dong; Wen-Zhou Sun; Dong-Lu Zhao; Yue Yang

    2005-01-01

    AIM: To investigate the effect and mechanism of action of the nitric oxide synthase (NOS) inhibitor NG-nitro-L-arginine methyl ester (L-NAME) on invasion and metastasis of human colorectal cancer cell line SL-174T.METHODS: Human colorectal cancer cell line SL-174T was cultured and treated separately with four different dosages of L-NAME for 72 h. Nitric oxide (NO) production was measured with Griess reagent. The effect of L-NAME on invasion and migration of SL-174T cells were evaluated by using Transwell chambers attached with polycarbonate filters and reconstituted basement membrane (Matrigel).RT-PCR was performed to determine the mRNA levels of matrix metalloproteinase-2 (MMP-2) and tissue inhibitor metalloproteinase-2 (TIMP-2).RESULTS: L-NAME could significantly inhibit NO production of SL-174T in a dose-dependent manner. After being treated for 72 h with 0.2, 0.4, 0.8, and 1.0 mmol/L LNAME, respectively, the ability of the L-NAME treated SL174T cells to invade the reconstituted basement membrane decreased significantly (t = 8.056, P<0.05;t= 14.467, P<0.01;t= 27.785, P<0.01;and t= 29.405,P<0.01, respectively) and the inhibition rates were 10.29%,19.62%, 34.08%, and 42.23%, respectively. Moreover,L-NAME could inhibit migration of SL-174T cells, and the inhibition rates were 20.76%, 24.95%, 39.43%, and 46.85% for L-NAME at 0.2, 0.4, 0.8, and 1.0 mmol/L,respectively (t = 15.116, P<0.01). In addition, after treatment with L-NAME, expression of MMP-2 mRNA was significantly decreased (t = 71.238, P<0.01) and that of TIMP-2 mRNA was markedly increased (t = -13.020,P<O.01).CONCLUSION: L-NAME exerts anti-invasive and antimetastatic effects on SL-174T cell line via downregulating MMP-2 mRNA expression and upregulating TIMP-2 mRNA expression.

  11. Influence of NG-nitro-L-arginine methyl ester on clinical and biochemical effects of methylene blue in pentylenetetrazole-evoked convulsions

    Directory of Open Access Journals (Sweden)

    Jelenković Ankica

    2012-01-01

    Full Text Available Background/Aim. Despite years of research in a number of experimental models the question whether nitric oxide (NO and methylene blue (MB have pro- or anticonvulsant effects remains to be fully resolved. Methods. In adult Wistar rats the influence of a nonselective inhibitor of nitric oxide synthase NG-nitro-L-arginine methyl ester (LNAME, 10µg on clinical and biochemical effects of MB (10µg given before the intraperitoneally administered chemical convulsant pentylenetetrazole (PTZ, 80 mg/kg was examined. MB and L-NAME were applied intracerebroventricularly. PTZ application was followed by a 4- minute observation time, after which rats were sacrificed and elements of oxido-reductive balance were measured in a crude mitochondrial fraction of forebrain cortex, hippocampus and striatum. Results. Convulsive responses (forelimb dystonia - FLD, generalised clonic- and clonic-tonic convulsions - GCC and GCTC respectively were observed in all rats received PTZ, together with significantly decreased lipid peroxidation in the forebrain cortex and striatum and increased superoxide dismutase activity in the hippocampus, in comparison to controls (saline treated. It was registered anticonvulsant effects of L-NAME pretreatment. However, these effects were insignificant. In the hippocampus of these animals there was decreased lipid peroxidation (p < 0.01, p < 0.05 vs saline-treated and PTZ-treated rats, respectively and reverted PTZ-induced increase of superoxide dismutase activity. But MB individually pretreatment significantly decreased the incidence of CTCs and GCCs (FLD: p = 0.0513, prolonged the convulsive latent time for FLD, GCTCs and GCCs, in all the examined brain regions increased lipid peroxidation and decreased the level of superoxide anion. Administration of L-NAME 10 minutes before MB reverted all MB-evoked clinical and biochemical effects. Conclusion. Methylene blue applied individually before PTZ has strong anticonvulsant effects that were

  12. Is it necessary to perform control diagnostic 131I whole body scan after remnant ablation in differentiated thyroid carcinoma patients who have stimulated Tg levels under 2 ng/ml?

    International Nuclear Information System (INIS)

    Full text of publication follows. Introduction: the aim of this retrospective study is to evaluate whether diagnostic I131 whole body scan (DWBS) performed 6-12 months after thyroid remnant ablation is necessary or not in differentiated thyroid carcinoma (DTC) patients with thyroglobulin (Tg) levels under 2 ng/ml and negative anti-Tg antibodies. Materials and methods: the study included 812 DTC patients undergoing the first control DWBS with Tg levels under 2 ng/ml and negative anti-Tg antibodies in the hypothyroid state, 6 months after post-surgical radio-ablation of residual thyroid tissue. Second DWB was performed 18 months after ablative I131 treatment (AIT) to 572 patients who had negative first control DWBS. These 572 patients had a stimulated Tg level under 2 ng/ml at the 18. month control. Results: the first control DWBS, 6 months after thyroid ablation, was negative in 789 (97.2%) patients and was positive for minimal residual uptake in the thyroid bed in 23 (2.8%) patients. 9 of the 23 patients with residual thyroid bed uptake, received a second dose of radioiodine therapy for complementary ablation of residual thyroid. 14 patients were not retreated. Second DWBS performed 18 months after AIT, was negative in all patients. Conclusion: our data suggest that in patients with stimulated Tg level under 2 ng/ml, DWBS performed 6 months after AIT, is informative only in minority of patients for residual uptake in the thyroid bed which is usually clinically not relevant. Therefore, we suggest that the DWBS may be avoided in patients with stimulated Tg level under 2 ng/ml. (authors)

  13. 骨髓间充质干细胞移植影响慢性脑缺血大鼠认知功能及海马区Nogo-A 、NgR 的表达%Transplantation of bone marrow mesenchymal stem cells improves cognitive function of rats with chronic cerebral ischemia and decreases Nogo-A and NgR protein expression in the hippocampus

    Institute of Scientific and Technical Information of China (English)

    王燕琳; 闫禹; 宋波; 张惠莉; 龚光明; 陈思; 方慧; 许予明

    2012-01-01

    BACKGROUND: Transplantation of bone marrow mesenchymal stem cells can decrease Nogo-A and NgR expression in rats with spinal cord injury and cerebral infarction. OBJECTIVE: To investigate the effects of transplantation of bone marrow mesenchymal stem cells on cognitive function of rats with chronic cerebral ischemia and Nogo-A and NgR protein expression in the hippocampus. METHODS: Thi rty Sprague-Dawley rats were randomly divided into sham-surgery, model and bone marrow mesenchymal stem cells groups. Rats in the latter two groups were established into model of chronic cerebral ischemia by permanent ligation of bilateral common carotid artery. In the bone marrow mesenchymal stem cells group, at 7 days after chronic cerebral ischemia induction, bone marrow mesenchymal stem cells (1×107) isolated by ficoll density gradient centrifugation were transfused into rats via the tailvein. RESULTS AND CONCLUSION: Compared with sham-surgery group, the average escape latency was significantly shortened (P < 0.01), the number that rats passed through the platform was significantly increased (P < 0.05), and Nogo-A and NgR expression in the hippocampal ti ssue was significantly decreased (P < 0.05) in the bone marrow mesenchymal stem cells group. These findings suggest that transplantation of bone marrow mesenchymal stem cells protects spatial learning and memory abilities of rats with chronic cerebral ischemia. This effect is possibly related to decreased Nogo-A and NgR protein expression.%背景:骨髓间充质干细胞移植可降低脊髓损伤及脑梗死大鼠的Nogo-A 及NgR 的表达.目的:观察骨髓间充质干细胞移植对慢性脑缺血大鼠认知功能及海马区Nogo-A 及NgR 蛋白的表达.方法:将30 只SD大鼠随机等分为假手术组、模型组及骨髓间充质干细胞组,后2 组采用双侧颈总动脉永久结扎法建立大鼠慢性脑缺血模型,骨髓间充质干细胞组在建模7 d后尾静脉注射移植用ficoll密度梯度离心法分

  14. 蛋白磷酸酯酶-1和-2A抑制剂对NG108-15细胞tau蛋白磷酸化的影响%EFFECT OF PROTEIN PHOSPHATASE-1 AND 2A INHIBITOR ON TAU PROTEIN PHOSPHORYLATION OF NG108-14 CELL

    Institute of Scientific and Technical Information of China (English)

    朱粹青; 曹小定

    2002-01-01

    Alzheimer痴呆(AD)的主要脑病理变化之一为由超磷酸化的tau蛋白组成的神经原纤维缠结(Neurofibrillary tangle,NFT).AD的Tau蛋白异常磷酸化与蛋白磷酸酯酶(PP)-2A和-1缺陷有关.本文首先用免疫印迹法显示NG含两大组分的tau蛋白.MTT方法观察到PP-2A和PP-1抑制剂冈田酸(Okadaic acid,OA)处理NG108-14细胞能导致细胞代谢明显下降,同时免疫印迹法显示OA能导致的NG细胞Tau蛋白磷酸化.该研究为建立AD样蛋白磷酸酯酶缺陷引起的tau蛋白磷酸化的细胞模型奠定了基础.

  15. ng i jazzgenren på gymnasienivå : En intervjustudie av fyra sångpedagogers syn på undervisning och arbetssätt inom genreområdet jazz

    OpenAIRE

    Waite, Nellie

    2013-01-01

    Syftet med studien är att få ökad insikt i hur sånglärare med utgångspunkt i kursplanens mål om genrebreddning hanterar genreområdet jazzsång i sångundervisningen på gymnasieskolans estetiska program. Genom att intervjua fyra sångpedagoger verksamma på gymnasiets estetiska program har jag tagit reda på vad som enligt dem kan betecknas som ett genreenligt sound eller genrespecifika egenskaper speciellt viktiga för jazzsång, hur dessa kunskaper kan förmedlas till eleverna samt hur sångpedagoger...

  16. Contribuição da densidade do PSA para predizer o câncer da próstata em pacientes com valores de PSA entre 2,6 e 10,0 ng/ml

    OpenAIRE

    Hugo Alexandre Sócrates de Castro; Wagner Iared; David Carlos Shigueoka; José Eduardo Mourão; Sérgio Ajzen

    2011-01-01

    OBJETIVO: Estudar o perfil dos pacientes submetidos a biópsia prostática, determinando possíveis padrões que, associados aos níveis de PSA entre 2,6 e 10,0 ng/ml, possam levar a uma diminuição de biópsias desnecessárias. MATERIAIS E MÉTODOS: De 2007 a 2009, foi realizado um estudo transversal com 1.282 indivíduos submetidos a biópsia prostática e que apresentavam níveis de PSA entre 2,6 e 10,0 ng/ml. RESULTADOS: A prevalência de câncer foi de 28,6%. Pacientes com câncer eram, em média, mais i...

  17. Quantifying the Value of Investing in Distributed Natural Gas and Renewable Electricity Systems as Complements: Applications of Discounted Cash Flow and Real Options Analysis with Stochastic Inputs

    Energy Technology Data Exchange (ETDEWEB)

    Pless, Jacquelyn; Arent, Douglas J.; Logan, Jeffrey; Cochran, Jaquelin; Zinaman, Owen

    2016-10-01

    One energy policy objective in the United States is to promote the adoption of technologies that provide consumers with stable, secure, and clean energy. Recent work provides anecdotal evidence of natural gas (NG) and renewable electricity (RE) synergies in the power sector, however few studies quantify the value of investing in NG and RE systems together as complements. This paper uses discounted cash flow analysis and real options analysis to value hybrid NG-RE systems in distributed applications, focusing on residential and commercial projects assumed to be located in the states of New York and Texas. Technology performance and operational risk profiles are modeled at the hourly level to capture variable RE output and NG prices are modeled stochastically as geometric Ornstein-Uhlenbeck (OU) stochastic processes to capture NG price uncertainty. The findings consistently suggest that NG-RE hybrid distributed systems are more favorable investments in the applications studied relative to their single-technology alternatives when incentives for renewables are available. In some cases, NG-only systems are the favorable investments. Understanding the value of investing in NG-RE hybrid systems provides insights into one avenue towards reducing greenhouse gas emissions, given the important role of NG and RE in the power sector.

  18. Bradykinin-activated transmembrane signals are coupled via N/sub o/ or N/sub i/ to production of inositol 1,4,5-trisphosphate, a second messenger in NG108-15 neuroblastoma-glioma hybrid cells

    International Nuclear Information System (INIS)

    The addition of bradykinin to NG108-15 cells results in a transient hyperpolarization followed by prolonged cell depolarization. Injection of inositol 1,4,5-trisphosphate or Ca2+ into the cytoplasm of NG108-15 cells also elicits cell hyperpolarization followed by depolarization. Tetraethylammonium ions inhibit the hyperpolarizing response of cells to bradykinin or inositol 1,4,5-trisphosphate. Thus, the hyperpolarizing phase of the cell response may be due to inositol 1,4,5-trisphosphate-dependent release of stored 45Ca-labelled Ca2+ into the cytoplasm, which activates Ca2+-dependent K+ channels. The depolarizing phase of the cell response to bradykinin is due largely to inhibition of M channels, thereby decreasing the rate of K+ efflux from cells and, to a lesser extent, to activation of Ca2+-dependent ion channels and Ca2+ channels. In contrast, injection of inositol 1,4,5-trisphosphate or Ca2+ into the cytosol did not alter M channel activity. Incubation of NG108-15 cells with pertussis toxin inhibits bradykinin-dependent cell hyperpolarization and depolarization. Bradykinin stimulates low K/sub m/ GTPase activity and inhibits adenylate cyclase in NG108-15 membrane preparations but not in membranes prepared from cells treated with pertussis toxin. These results show that [bradykinin-receptor] complexes interact with N/sub o/ or N/sub i/ and suggest that N/sub o/ and/or N/sub i/ mediate the transduction of signals from bradykinin receptors to phospholipase C and adenylate cyclase

  19. Representasi Gaya Hidup Wanita Metropolis Dalam Novel (Analisis Wacana Teun A. Van Dijk Mengenai Representasi Gaya Hidup Wanita Metropolis Dalam Novel Indiana Chronicles “Blues” Karya Clara Ng)

    OpenAIRE

    Rahmatika, Khairia

    2010-01-01

    Penelitian ini berjudul “Representasi Gaya Hidup Wanita Dalam Novel” (Analisis Wacana Teun A. van Dijk Mengenai Representasi Gaya Hidup Wanita Metropolis Dalam Novel Indiana Cronicle Blues Karya Clara Ng). Gaya hidup adalah pola hidup seseorang di dunia yang merupakan ciri sebuah dunia modern, atau yang biasa juga disebut modernitas. Kebutuhan akan status dan terpaan budaya asing ini mengakibatkan merebaknya gaya hidup metropolis yang cenderung permisif dan mengedepankan kemewahan daripada ke...

  20. Three-step laser excitation of the 6p3/2ns, nd, ng autoionizing Rydberg levels via the 6p5f 1/2[5/2]2 level of lead

    Science.gov (United States)

    Ahad, A.; Nadeem, A.; Bhatti, S. A.; Baig, M. A.

    2005-03-01

    Odd parity autoionizing Rydberg levels of atomic lead in the energy region above the 6p1/2 ionization threshold have been investigated using three-step laser excitation in conjunction with an atomic beam apparatus. The 6p3/2ns (J = 1, 2), 6p3/2nd (J = 1, 2, 3) and 6p3/2ng (J = 2, 3) levels have been observed from the 6p5f 1/2[5/2]2 intermediate level. Energy values and FWHM of forty levels belonging to the 6p3/2ns, 6p3/2nd and 6p3/2ng configurations are presented. Six levels based on the 6p3/2ng (5, 13 ≤n ≤15) configurations and three levels attached to the 6p3/28d configuration are reported for the first time. The present study of the low-lying autoionizing levels attached to the 6p3/25g (J = 2, 3) configuration completes the series adjacent to the 6p1/2 limit.

  1. Criticality assessment of terrorism related events at different time scales TENSOR clusTEriNg terroriSm actiOn pRediction

    OpenAIRE

    Sormani, R

    2016-01-01

    Law Enforcement Agencies (LEAs) are nowadays taking advantage of a wide range of information and intelligence sources (e.g., human intelligence (HUMINT), open source intelligence (OSINT), image analysis (IMINT)) to anticipate potential terroristic actions. Urban environments are nowadays associated with a wide range of vulnerabilities, which create fertile ground for terrorists planning actions against assets and/or citizens. These vulnerabilities stem from the characteristics of the urban en...

  2. Comparison of the Abbott m2000 RealTime CT Assay and the Cepheid GeneXpert CT/NG Assay to the Roche Amplicor CT Assay for Detection of Chlamydia trachomatis in Ocular Samples from Tanzania

    OpenAIRE

    Dize, Laura; West, Sheila; Williams, James A; Van Der Pol, Barbara; Quinn, Thomas C.; Gaydos, Charlotte A.

    2013-01-01

    The GeneXpert CT/NG assay (GeneXpert) and the Abbott m2000 RealTime CT (m2000) assay were compared to Amplicor for detecting ocular Chlamydia trachomatis. Discordant specimens were tested by the Aptima CT assay. The m2000 assay sensitivity was 100% (95% confidence interval [CI], 90% to 100%), and specificity was 98.46% (95% CI, 95.2% to 99.2%); GeneXpert sensitivity was 100% (95% CI, 90% to 100%), and specificity was 100% (95% CI, 98.1% to 100%). The m2000 and GeneXpert assays appear to perfo...

  3. A new species of Foza Reed & Cumberlidge, 2006 from northern Madagascar (Decapoda, Brachyura, Potamoidea, Potamonautidae, with a redescription of F. goudoti (H. Milne Edwards, 1853 comb. n., and comments on Skelosophusa prolixa Ng & Takeda, 1994

    Directory of Open Access Journals (Sweden)

    Neil Cumberlidge

    2009-08-01

    Full Text Available Foza ambohitra sp. n. is described from Ambohitra, Antsiranana Province, northern Madagascar at 421 m elevation. This species is distinguished by characters of the carapace, the male anterior thoracic sternum, and the form of the male major cheliped and first gonopod. Thelphusa goudoti H. Milne Edwards, 1853, is transferred to Foza Reed & Cumberlidge, 2006, and redescribed, and a key to the three species of this genus is provided. Comments on the rare cave crab Skelosophusa prolixa Ng & Takeda, 1994, from Antsiranana Province are also included based on newly obtained material.

  4. Cratoxylum formosum (Jack) Dyer ssp. pruniflorum (Kurz) Gogel. (Hóng yá mù) extract induces apoptosis in human hepatocellular carcinoma HepG2 cells through caspase-dependent pathways

    OpenAIRE

    Nonpunya, Apiyada; Weerapreeyakul, Natthida; Barusrux, Sahapat

    2014-01-01

    Background Cratoxylum formosum (Jack) Dyer ssp. pruniflorum (Kurz) Gogel. (Hóng yá mù) (CF) has been used for treatment of fever, cough, and peptic ulcer. Previously, a 50% ethanol-water extract from twigs of CF was shown highly selective in cytotoxicity against cancer cells. This study aims to investigate the molecular mechanisms underlying the apoptosis-inducing effect of CF. Methods The cytotoxicity of CF was evaluated in the human hepatocellular carcinoma (HCC) HepG2 cell line in comparis...

  5. Aerodynamická analýza a návrh úprav podvozkové gondoly letounu L 410 NG

    OpenAIRE

    Pukl, Marek

    2013-01-01

    Tato diplomová práce se zabývá analýzou proudění kolem podvozkové gondoly letounu L 410 a její následnou aerodynamickou optimalizací. V první části je v rámci kalibrační úlohy zpracována známá geometrie, jejíž parametry byly ověřeny tunelovým měřením, následující části pak zahrnují návrh vlastní optimální geometrie, tvorbu výpočetní sítě a její výpočet a konečné zhodnocení výsledků. This diploma thesis deals with the flow analysis around the landing gear nacelle of L 410 and with its follo...

  6. Ideais e realidade: uma aula reservada para crianças autistas bilíngües = Ideals and reality: a self-contained class for bilingual autistic children

    Directory of Open Access Journals (Sweden)

    Ebsworth, Miriam Eisenstein

    2009-01-01

    Full Text Available Este estudo qualitativo abordou as experiências de crianças bilíngües, selecionadas com desordem do espectro autista em uma sala de aula de Educação Infantil Especial Bilíngüe. Os dados incluíram observação ao vivo, gravações em vídeo e entrevistas com os funcionários e pais. Apesar da dedicação dos professores, dos funcionários e da família, os desafios incluíram limitações lingüísticas de clínicos gerais, uso inconsistente de espanhol e/ou inglês, falta de compreensão dos pais sobre a natureza do autismo e demanda irreal de funcionários. O ambiente da barulhenta sala de aula, às vezes refletia aprendizagem, que freqüentemente significava transtorno. A preparação profissional de professores também era inadequada

  7. Comparison of Neisseria gonorrhoeae Multiantigen Sequence Typing and porB Sequence Analysis for Identification of Clusters of N. gonorrhoeae Isolates▿ †

    OpenAIRE

    Liao, Mingmin; Helgeson, Sarah; Gu, Wei-Ming; Yang, Yang; Jolly, Ann M.; Dillon, Jo-Anne R.

    2008-01-01

    porB DNA sequence analysis and Neisseria gonorrhoeae multiantigen sequence typing (NG-MAST) methods were compared for their abilities to discriminate strains and to identify epidemiologically congruent pairs of N. gonorrhoeae. Both methods provided high-level discrimination of strains. NG-MAST further differentiated large porB-based clusters. However, considerations of cost suggest that porB DNA sequence analysis is a useful tool for preliminary molecular analysis of the epidemiology of N. go...

  8. The Songbird Neurogenomics (SoNG Initiative: Community-based tools and strategies for study of brain gene function and evolution

    Directory of Open Access Journals (Sweden)

    Lewin Harris A

    2008-03-01

    Full Text Available Abstract Background Songbirds hold great promise for biomedical, environmental and evolutionary research. A complete draft sequence of the zebra finch genome is imminent, yet a need remains for application of genomic resources within a research community traditionally focused on ethology and neurobiological methods. In response, we developed a core set of genomic tools and a novel collaborative strategy to probe gene expression in diverse songbird species and natural contexts. Results We end-sequenced cDNAs from zebra finch brain and incorporated additional sequences from community sources into a database of 86,784 high quality reads. These assembled into 31,658 non-redundant contigs and singletons, which we annotated via BLAST search of chicken and human databases. The results are publicly available in the ESTIMA:Songbird database. We produced a spotted cDNA microarray with 20,160 addresses representing 17,214 non-redundant products of an estimated 11,500–15,000 genes, validating it by analysis of immediate-early gene (zenk gene activation following song exposure and by demonstrating effective cross hybridization to genomic DNAs of other songbird species in the Passerida Parvorder. Our assembly was also used in the design of the "Lund-zfa" Affymetrix array representing ~22,000 non-redundant sequences. When the two arrays were hybridized to cDNAs from the same set of male and female zebra finch brain samples, both arrays detected a common set of regulated transcripts with a Pearson correlation coefficient of 0.895. To stimulate use of these resources by the songbird research community and to maintain consistent technical standards, we devised a "Community Collaboration" mechanism whereby individual birdsong researchers develop experiments and provide tissues, but a single individual in the community is responsible for all RNA extractions, labelling and microarray hybridizations. Conclusion Immediately, these results set the foundation for a

  9. Effect of affordable technology on physical activity levels and mobility outcomes in rehabilitation: a protocol for the Activity and MObility UsiNg Technology (AMOUNT) rehabilitation trial

    Science.gov (United States)

    Hassett, Leanne; van den Berg, Maayken; Lindley, Richard I; Crotty, Maria; McCluskey, Annie; van der Ploeg, Hidde P; Smith, Stuart T; Schurr, Karl; Killington, Maggie; Bongers, Bert; Howard, Kirsten; Heritier, Stephane; Togher, Leanne; Hackett, Maree; Treacy, Daniel; Dorsch, Simone; Wong, Siobhan; Scrivener, Katharine; Chagpar, Sakina; Weber, Heather; Pearson, Ross; Sherrington, Catherine

    2016-01-01

    Introduction People with mobility limitations can benefit from rehabilitation programmes that provide a high dose of exercise. However, since providing a high dose of exercise is logistically challenging and resource-intensive, people in rehabilitation spend most of the day inactive. This trial aims to evaluate the effect of the addition of affordable technology to usual care on physical activity and mobility in people with mobility limitations admitted to inpatient aged and neurological rehabilitation units compared to usual care alone. Methods and analysis A pragmatic, assessor blinded, parallel-group randomised trial recruiting 300 consenting rehabilitation patients with reduced mobility will be conducted. Participants will be individually randomised to intervention or control groups. The intervention group will receive technology-based exercise to target mobility and physical activity problems for 6 months. The technology will include the use of video and computer games/exercises and tablet applications as well as activity monitors. The control group will not receive any additional intervention and both groups will receive usual inpatient and outpatient rehabilitation care over the 6-month study period. The coprimary outcomes will be objectively assessed physical activity (proportion of the day spent upright) and mobility (Short Physical Performance Battery) at 6 months after randomisation. Secondary outcomes will include: self-reported and objectively assessed physical activity, mobility, cognition, activity performance and participation, utility-based quality of life, balance confidence, technology self-efficacy, falls and service utilisation. Linear models will assess the effect of group allocation for each continuously scored outcome measure with baseline scores entered as a covariate. Fall rates between groups will be compared using negative binomial regression. Primary analyses will be preplanned, conducted while masked to group allocation and use an

  10. Rahvapärane viiulimäng 20.sajandi esimesel poolel Tori ja Vändra viiuldajate näitel

    Directory of Open Access Journals (Sweden)

    Krista Sildoja

    2011-12-01

    Tori fiddlers. Imitating bagpipe is definitely one of the earliest of way ofplaying in terms of age in our old fiddle music. The other way of playing in duo performancesis simple polyphony based on functional harmony.Fiddlers made the melodies of Estonian type of waltzes and polkas more interestingand richer using polyphonic fiddle playing technique and embellishments of melodynotes. They used embellishments (mordents and upbeats when they “felt like it”.Such means make a piece of music airy and general impression of the playing technique rather masterly.The structures of the analysed fiddle pieces are simpler and also of a rather surprising (and random construction as compared to composed classical music. This is what makes the music under survey so exciting. Each piece offers something new and unexpected – prevalently the predictable and expected systematic thinking does not exist. In case of Estonian type of waltzes as well as polkas the most important idea is that the piece of music had a continuous metrum to support dancers in their dance. As a result of form analysis it appeared that different playthroughs of Estonian type of waltzes are freer in terms of their construction, or more improvised, polkas on the other hand are more stable. The dominant factor in the development of playthroughs proved to be the number of members in the performance. If it is a solo performance, the variations in different levels of form are more frequent than in case of a duo performance. It is also understandable as the co-play of at least two people requires previous agreement in approach to form.The playing style in traditional music, as a form of music learnt by ear, is quite individual in each player. At the same time it can be observed that the playing styles of two players living close to each other generally do not differ significantly. The same can be seen in the analysis of the fiddle players’ playing styles in the present research. The playing styles of fiddle

  11. Determination of the chromium content of laboratory rabbit skeletal muscles by neutron activation analysis

    International Nuclear Information System (INIS)

    The chromium content of skeletal muscle of laboratory rabbits has been determined using neutron activation analysis. The procedure for separation of 51 Cr by distillation of chromium oxychloride, described in the literature, has been improved. The arrangements necessary to minimize the chromium blank values are described. The main component of this blank is caused by the residual chromium contamination of the surface of the sample vial; typical values of this component are 0.049 ng Cr (without lyophilization) and 0.12 ng Cr (with lyophilization). The analyses of standard reference materials (SRM) yielded values of the chromium contents that are in agreement (I) with the certified value in the case of NBS Citrus Leaves, and (II) with the latest published value of 9.2+-2.5 ng/g in the case of IAEA animals muscle (H-4). NBS Orchard Leaves was found not to be an appropriate SRM for testing the method. In analyses of samples of thigh muscle of bastard rabbits chromium contents of 6.2-22.9 ng/g (fresh weight basis) were obtained. Comparison of these data with a previously found value of 1.2 ng/g, the literature value <7.1 ng/g and the value 2.5 ng/g for H-4 calculated on fresh weight basis indicated that the chromium contents of mammalian skeletal muscle might lie in a broad range, even for a subspecies. (orig./RB)

  12. Headspace Solid Phase Microextraction in Pesticide Residues Analysis:1. Optimisation of Extraction Conditions

    Directory of Open Access Journals (Sweden)

    Rada Đurović

    2007-01-01

    Full Text Available The method of headspace solid phase microextraction (HS/SPME was successfully used in a simultaneous multicomponent analysis of hexachlorobenzene (HCB, tefluthrin, heptachlor, aldrin, chlorpyrifos, fenthion and bifenthrin in aqueous medium. Measurementswere performed using a nonpolar polydimethyl siloxane (PDMS fiber. Detection and quantification were done by gas chromatography/mass spectrometry (GC/MS.Optimal conditions for HS/SPME were determined both by performing extraction at different temperatures and examining extraction time profiles at constant temperature. Optimal extraction temperature for each pesticide studied was determined as follows: 60°C for HCB and for heptachlor, 80°C for aldrin and for chlorpyrifos, fenthion and tefluthrin, and temperature exceeding 80°C for bifenthrin. For the pesticide mixture studied, 60°C was identified as the optimum extraction temperature.Based on the time profiles obtained, it was confirmed that satisfactory extraction sensitivity can be obtained even for extraction times shorter than the time required to reach a sorption equilibrium. This conclusion was confirmed by linear concentration profiles obtained for the following ranges: 0.05-10 ng/ml (HCB, 0.05-25 ng/ml (tefluthrin, 0.05-40 ng/ml (heptachlor, 0.05-40 ng/ml (aldrin, 0.05-25 ng/ml (chlorpyrifos, 0.05-25 ng/ml (fenthionand 0.05-25 ng/ml (bifenthrin.Relative standard deviation (RSD values for triplicate measurements did not exceed 15%.

  13. Neisseria gonorrhoeae antimicrobial susceptibility in Barcelona: penA, ponA, mtrR, and porB mutations and NG-MAST sequence types associated with decreased susceptibility to cephalosporins.

    Science.gov (United States)

    Serra-Pladevall, J; Barberá, M J; Rodriguez, S; Bartolomé-Comas, R; Roig, G; Juvé, R; Andreu, A

    2016-09-01

    The aims of this study were to determine the antimicrobial susceptibility of Neisseria gonorrhoeae (NG) in our area, to analyze the molecular mechanisms involved in cephalosporins resistance, and to undertake molecular typing of our NG strains. Antimicrobial susceptibility was determined using the Etest. The genes penA, mtrR, penB, and ponA were studied. Molecular typing was performed by N. gonorrhoeae multiantigen sequence typing. Of 329 strains analyzed in 2013, none showed high-level cephalosporin resistance, but 8.2 % had resistance to cefixime [minimum inhibitory concentration (MIC) > 0.125 μg/mL] and 0.6 % to ceftriaxone (MIC > 0.125 μg/mL). Azithromycin resistance was documented in 4.3 % and ciprofloxacin resistance in 49.2 %. Among 48 strains with an MIC ≥ 0.125 μg/mL to cefixime, 58.3 % showed the penA mosaic pattern XXXIV, 98 % a Leu → Pro substitution at position 421 of the ponA gene, 100 % amino acid changes at positions 101 and 102 of the PorB1b porin, and 87.5 % of strains an adenine deletion in the promoter region of the MtrC-D-E efflux pump. A significant difference between strains with and without decreased cephalosporin susceptibility (MIC ≥ 0.125 μg/mL) was observed for these four genes. Of the 48 strains with an MIC ≥ 0.125 μg/mL to cefixime, 43.8 % belonged to the genogroup G1407 and 27.1 % belonged to the genogroup G2400. A significant association of G1407 with decreased susceptibility (MIC ≥ 0.125 μg/mL) and G2992 with susceptibility was found, and also between G1407 and mosaic pattern XXXIV and between G2400 and A501T substitution in penA. The NG resistance rate in our area is higher than the median of Europe. We have detected the emergence of G2400, which may be a source of antimicrobial resistance. PMID:27255221

  14. PCR Reverse Hybridization Membrane Applications in Screening of CT,UU,NG Three Kinds of STD%PCR反向膜杂交法在CT、UU、NG三种性病筛查中的应用价值

    Institute of Scientific and Technical Information of China (English)

    王涛; 王鹏; 董丽

    2014-01-01

    Objective:To investigate the value of PCR reverse hybridization membrane application in screening of CT,UU,NG three kinds of STD. Method:1486 suspected patients with genitourinary tract infections were detected by PCR reverse hybridization membrane and fluorescence quantitative PCR(FQ-PCR),and test results were compared with culture results. Result:FQ-PCR detection of CT,UU,NG was higher than culture(P<0.01). PCR reverse hybridization membrane,culture and FQ-PCR positive rate difference was not statistically significant. PCR reverse hybridization membrane,FQ-PCR detection sensitivity difference was not statistically significant,but the film PCR reverse hybridization assay specificity was higher than FQ-PCR(P<0.01). Conclusion:PCR reverse hybridization in screening of CT,UU,NG,is accurate,rapid,and specific is higher than FQ-PCR,worthy of promotion apply.%目的:探讨PCR反向膜杂交法在CT、UU、NG三种性病筛查中的应用价值。方法:对1486例疑为泌尿生殖道感染患者分别采用PCR反向膜杂交法及荧光定量PCR(FQ-PCR)检测,并将检测结果与培养结果比较。结果:FQ-PCR检测CT、UU、NG阳性率高于培养法,比较差异有统计学意义(P<0.01);PCR反向膜杂交法与培养法及FQ-PCR检测阳性率比较差异均无统计学意义;PCR反向膜杂交法、FQ-PCR检测敏感性比较差异无统计学意义,但PCR反向膜杂交法检测特异性高于FQ-PCR,比较差异有统计学意义(P<0.01)。结论:PCR反向膜杂交法筛查CT、UU、NG准确,快速,且筛查特异性高于FQ-PCR,值得临床推广适用。

  15. Effects of Ginsenoside Rg1 on the Expression of Toll-Like Receptor 3, 4 and Their Signalling Transduction Factors in the NG108-15 Murine Neuroglial Cell Line

    Directory of Open Access Journals (Sweden)

    Bao-Sheng Zhao

    2014-10-01

    Full Text Available As one of the most important components of Panax ginseng, ginsenoside Rg1 has certain anti-aging effects, improving the activity of learning and memory. Studies have showed that ginsenoside Rg1 improves the memory impairment associated with Alzheimer’s disease (AD. In this study, the effects of ginsenoside Rg1 were investigated through the activity of toll-like receptor (TLR 3, TLR4 and their signaling transduction pathways in amyloid β peptide 25–35 (Aβ25–35 induced AD cell model. Thus we investigated several critical components of the TLR pathway. The neuroglial cell line NG108-15 was stimulated with or without Aβ25–35, while different concentrations of ginsenoside Rg1 were administered. After 24 h, tumor necrosis factor-α (TNF-α, interferon-β (IFN-β in cell supernatant and inducible nitric oxide synthase (iNOS in cell lysate supernatant were measured with enzyme-linked immunosorbent assays (ELISAs. The mRNA and protein expression of TLR3, TLR4, nuclear factor kappa B (NF-κB and tumor necrosis factor receptor-associated factor-6 (TRAF-6 were detected by real-time PCR and western blot methods, respectively. The experimental results showed that Aβ25–35 could markedly raise the level of TNF-α, IFN-β and iNOS, and increase the expressions of mRNA and TLR3, TLR4, NF-κB and TRAF-6 protein in the NG108-15 cells. At the same time, the ginsenoside Rg1 significantly reduced the expressions of proteins and mRNA of TLR3, TLR4, NF-κB and TRAF-6, and down-regulated the levels of TNF-α, IFN-β of cell supernatant and iNOS of cell lysate supernatant in a concentration-dependent manner. In conclusion, ginsenoside Rg1 has good activity for suppressing the signaling transduction pathway of TLR3 and TLR4, and decreasing the inflammation factors induced by Aβ25–35 in NG108-15 cells, and this may be the mechanism of ginsenoside Rg1 action in AD treatment, but more studies are needed to identify its specificity.

  16. Pre-combustion CO2 capture: Analysis of integrated reforming combined cycle

    OpenAIRE

    Nord, Lars Olof

    2010-01-01

    This thesis presents processes for reducing CO2 emissions from natural gas (NG) power plants, which could help attenuate the rise in atmospheric temperature. Objectives for the thesis work were process design and integration of NG pre-combustion CO2 capture plants, and evalution, through process simulation, of the concepts. An important aspect of the evaluation was investigation of plant flexibility, specifically off-design analysis. Contributions comprised detailed heat recovery steam genera...

  17. Pre-combustion CO2 capture: Analysis of integrated reforming combined cycle

    OpenAIRE

    Nord, Lars Olof

    2010-01-01

    This thesis presents processes for reducing CO2 emissions from natural gas (NG) power plants, which could help attenuate the rise in atmospheric temperature. Objectives for the thesis work were process design and integration of NG pre-combustion CO2 capture plants, and evalution, through process simulation, of the concepts. An important aspect of the evaluation was investigation of plant flexibility, specifically off-design analysis. Contributions comprised detailed heat recovery steam genera...

  18. NG含量对改性单基发射药燃烧性能的影响%Effect of NG Content on the Burning Performance of Modified Single-base Gun Propellant

    Institute of Scientific and Technical Information of China (English)

    李强; 闫光虎; 张玉成; 严文荣; 张江波; 赵晓梅; 刘强; 杜江媛

    2012-01-01

    The modified single-base gun propellant samples were prepared based on 5/7 single-base gun propellantwith high N content by dipping NG, then desensitizer NA polyester. In the sample preparing process the content ofNA was kept invariable and the mass content of NG was 5% ,10% and 15% , respectively. Three samples, |RN-a,RN-b and RN-c, were prepared. The effect of NG content on the burning performance of modified single-base gunpropellant was studied based on the closed bomb test and interior ballistic test with 30mm gun. The results showthat the burning progressivity of all the three samples is better than that of original 5/7 single-base gun propellant.Under equivalent maximum pressure, muzzle velocity of the RN-a, RN-b and RN-c propellant charges is increasedby 36. 9, 91. 7 and 57. 4m/s respectively compared to the original 5/7 single-base gun propellant. The performanceof modified single-base gun propellant can be optimized by adjusting the content of NG, so that the muzzle velocitycan be greatly increased.%以高氮量5/7单基发射药为基体药粒,通过先浸渍吸收NG、后吸收聚酯钝感剂NA的两步工艺制备出改性单基药,在制备过程中,NA含量恒定,浸渍NG的质量分数为5%、10%和15%,制备出3种NG含量的改性单基药样品RN-a、RN-b和RN-c.采用密闭爆发器试验和30 mm弹道炮内弹道试验,研究了NG含量对改性单基药燃烧性能的影响.结果表明,所制备的3种改性单基药样品的燃烧渐增性均优于基体药;在保持最大膛压不增加的情况下,弹丸初速较5/7单基药装药分别提高36.9、91.7和57.4m/s.在制备工艺过程中,通过调节NG的含量,能够改善改性单基药的燃烧性能,实现大幅度提高炮口初速的目的.

  19. Analysis

    CERN Document Server

    Maurin, Krzysztof

    1980-01-01

    The extraordinarily rapid advances made in mathematics since World War II have resulted in analysis becoming an enormous organism spread­ ing in all directions. Gone for good surely are the days of the great French "courses of analysis" which embodied the whole of the "ana­ lytical" knowledge of the times in three volumes-as the classical work of Camille Jordan. Perhaps that is why present-day textbooks of anal­ ysis are disproportionately modest relative to the present state of the art. More: they have "retreated" to the state before Jordan and Goursat. In recent years the scene has been changing rapidly: Jean Dieudon­ ne is offering us his monumentel Elements d'Analyse (10 volumes) written in the spirit of the great French Course d'Analyse. To the best of my knowledge, the present book is the only one of its size: starting from scratch-from rational numbers, to be precise-it goes on to the theory of distributions, direct integrals, analysis on com­ plex manifolds, Kahler manifolds, the theory of sheave...

  20. The fluidity of Chinese Americans'ethnic and sexual identity in Fae Myenne Ng's Bone%伍慧明《骨》中美籍华人种族及性别身份流变

    Institute of Scientific and Technical Information of China (English)

    周燕萍

    2014-01-01

    Identity has been a focus in post-colonial studies .Chinese American writer Fae Myenne Ng's debut Bone excavates Chinese Americans'ethnic and sexual identification under feminist , anti-colonial and anti-racist move-ments.Using Stuart Hall's conception of fluid identity as the theoretical foundation ,and Bone as text,this thesis in-tends to explore Fae Myenne Ng'effort in deconstructing ,constructing and reconstructing Chinese Americans ethnic and sexual identities based on different social ,historical and political backgrounds ,which well illustrates post-colo-nial identity is not in a static state ,but in a dynamic state with constant changing transformations .%身份问题一直以来是后殖民研究中的焦点。美籍华裔作家伍慧明的首部作品《骨》因揭示了民权运动、女权运动背景下的美籍华裔寻找身份的苦痛历程而备受关注。本文以斯图亚特·霍尔的身份流变论为理论,通过对《骨》的文本分析,探寻不同社会、历史及政治背景下美籍华人民族及性别身份的解构、建构与重建,例证后殖民身份不是静态的,而是处于不断流变的过程当中。

  1. Algumas observações pragmáticas na construção de sentidos na produção escrita: um estudo de caso bilíngüe

    Directory of Open Access Journals (Sweden)

    Lucia Rottava

    2002-01-01

    Full Text Available Este estudo investiga, sob uma perspectiva pragmática (cf. Thomas,1983 e Mey,mimeo, 1985 e 1993, a construção de sentidos na produção escrita por um falante bilíngüe (cf. Skutnabb-Kangas, 1981 e Romaine, 1995 , cujas línguas focalizadas são o português e o espanhol. A língua materna do sujeito é o português, mas esteve em contexto em que a língua oficial é o espanhol. Os dados são dois textos escritos produzidos em situações diferentes. Os resultados sugerem que as produções escritas do sujeito refletem as práticas sociais de que participa e representam o lugar em que seu conhecimento pragmático interage com a língua alvo. Uma contribuição deste estudo diz respeito à reflexão a respeito do papel do professor como interlocutor institucionalmente determinado que parece estar em conflito com o sujeito/escritor bilíngüe.This study investigates, on a pragmatical perspective, the making of meanings in the written productions of a Portuguese-Spanish bilingual speaker. The subject's mother language is Portuguese. However, he has lived in a context where Spanish is the official language. The data emerge from two written texts produced in different situations. The results suggest that the written productions reflect the social practice in which the subject takes part. They also represent the place where his pragmatical knowledge interacts with the target language. This study contributes to a reflection on the role of the teacher as an interlocutor determined by the institution who seems to be in conflict with the bilingual subject/writer.

  2. Organização e qualidade da assistência à saúde dos índios Kaingáng do Rio Grande do Sul, Brasil

    Directory of Open Access Journals (Sweden)

    Hökerberg Yara Hahr Marques

    2001-01-01

    Full Text Available Este estudo pretende avaliar o atendimento à saúde prestado aos índios Kaingáng do Rio Grande do Sul. Os óbitos evitáveis por assistência básica de saúde dos indígenas, ocorridos entre 1985 e 1995, foram comparados aos óbitos do Estado do Rio Grande do Sul. Os dados secundários relativos aos serviços de saúde foram complementados por entrevistas realizadas em campo com lideranças indígenas e com funcionários das instituições envolvidas na assistência à saúde do índio. Foi utilizado o Sistema de Informações Geográficas, visando relacionar a distribuição dos óbitos ao grau de acesso aos serviços de saúde. As áreas indígenas Kaingáng estão ligadas por estradas pavimentadas a municípios que contam com, pelo menos, um centro de saúde, ou ainda, hospitais de pequeno porte. O segundo nível de atenção situa-se em Palmeiras das Missões e Frederico Westphallen e o terceiro nível, em Passo Fundo. O que diferencia estas áreas indígenas do restante do Estado do Rio Grande do Sul é a quantidade de óbitos evitáveis pela assistência básica de saúde, tais como as causas mal definidas, a desnutrição, a tuberculose e o câncer cérvico-uterino.

  3. ng tulega / Kai Lobjakas

    Index Scriptorium Estoniae

    Lobjakas, Kai, 1975-

    2009-01-01

    Eesti Keraamikute Liidu 9. rahvusvaheline keraamikasümpoosion Kohilas Tohisoo mõisas 6.-25. juulini. Lühidalt Jari Vesterineni ja Nanna Bayeri (Soome), Kamila Dziedzici (Poola), Anne Türni ja Marju Vaheri (Eesti), Nelli Isupova (Ukraina) ning Anthony Stellaccio (USA) töödest

  4. Renessanslik vetemäng / Tiina Tammet

    Index Scriptorium Estoniae

    Tammet, Tiina, 1971-

    2009-01-01

    Rooma lähedal Tivoli linnakeses asuvast Itaalia 16. sajandi renessansiaegse palee ja aiakunsti pärlist Villa d'Este, mis on UNESCO maailmapärandi nimekirjas. Pargi ja palee arhitektid Pirro Ligorio ja Alberto Galvani, purskkaevude autoriks skulptor Gianlorenzo Bernini

  5. ng ja maagia / Doris Kareva

    Index Scriptorium Estoniae

    Kareva, Doris, 1958-

    2001-01-01

    20. nov. 2001 arutati Tartu Kirjanike Maja kriitikaseminaril Mehis Heinsaare "Härra Pauli kroonikaid" (2001). Ka Valdo Valperi näitusest Illegaardi galeriis "Eesti kirjandus enne ja pärast Mehis Heinsaart"

  6. ng õpetajate palganumbritega / Olav Aarna

    Index Scriptorium Estoniae

    Aarna, Olav, 1942-

    2005-01-01

    Ilmunud ka: Vooremaa 5. nov., lk. 2, Harjumaa 8. nov., lk. 2, Hiiu Leht 8. nov., lk. 2, Virumaa Teataja 8. nov., lk. 7, Lääne Elu 8. nov., lk. 2, Võrumaa Teataja 8. nov. lk. 2, Põhjarannik Severnoje Poberezhje 11. nov. lk. 2, Valgamaalane 12. nov. lk. 2, Pärnu Postimees 16. nov. lk. 15, Sakala 29. nov. lk. 2. Riigikogu kultuurikomisjoni esimees süüdistab haridusminister Mailis Repsi segaduse tekitamises õpetajate palgapoliitikas

  7. Sammenhæng i ungdomsuddannelserne

    DEFF Research Database (Denmark)

    Laursen, Erik; Rasmussen, Palle

    2009-01-01

    For efterhånden flere år siden formulerede regeringen en målsætning om, at 95 pct. af alle unge skal gennemføre en ungdomsuddannelse. I dag er der ikke meget der tyder på, at vi nærmer os målet. Tværtimod. I de sidste 15 år har andelen der gennemfører en ungdomsuddannelse ligget omkring 80 pct., ...

  8. HEALTHY AND SAFETY SWİMMİNG

    OpenAIRE

    Süleyman Ceylan

    2005-01-01

    Swimming is a sport which has own rules, styles, and fields, however, is one of the most performed avocation as amateur and a joke especially at summer months. Although one of the most beneficial sports, swimming can cause a number of several health problems such as infectious diseases, allergic events, or traumas, if it is not done at adequate conditions and eligible style. In this paper, the factors such as preparing to swimming, health and safety features of swimming areas, important healt...

  9. In/compatible Imagi(ni)ng

    DEFF Research Database (Denmark)

    Kristensen, Thomas Bjørnsten

    2012-01-01

    The article thematizes a certain logic of reproduction that follows from a point in art history where the construction of images became a process of assembling or coding discrete signals through systematic methods. A logic which is not concerned with imitation but with creating an interchangeable...

  10. Th? Imp?ct of F?n?nc??l R?port?ng of Sm?ll ?nd M?d?um S?z?d Ent?rpr?s?s ?n Econom?c D?v?lopm?nt of B?lk?n Countr??s

    OpenAIRE

    Artor Nuhiu; Arber Hoti; Lulzim Krasniqi

    2012-01-01

    Th? ?mport?nc? of ?nt?rn?l ?nd ?xt?rn?l ?ccount?ng ?s ? sourc? of ?nform?t?on for own?rs ?nd m?n?g?rs of sm?ll ?nt?rpr?s?s ?nd th??r d?ff?r?nt st?k?hold?rs ?s st??d?ly grow?ng. It ?s of cruc??l ?mport?nc? th?t th? ?ccount?ng syst?ms ?ppl??d by sm?ll ?nt?rpr?s?s m??t th??r ?ctu?l n??ds, prov?d?ng n?c?ss?ry ?nform?t?on y?t ?vo?d?ng unjust?f??d ?dm?n?str?t?v? burd?n. It ?s r?cogn?s?d th?t ?ppropr??t? ?ccount?ng ?nform?t?on ?s ?mport?nt for ? succ?ssful m?n?g?m?nt of ? bus?n?ss wh?th?r ?t ?s l?rg...

  11. Classıfıcatıon of Dıstrıct TR72 Towns wıth Fuzzy Clusterıng Analysıs Usıng Socıo-Economıc Data

    OpenAIRE

    Erilli, N. Alp

    2014-01-01

    In economy policies, socioeconomic indicators have an important place in determining the development levels. The determination and classification of the current social and economic structure of cities and districts are considerably important in analyzing the development of cities and districts in their probable development tendencies and in developing the regional development policies in parallel to this. It is essential to use fuzzy clustering analysis, whether clusters separated well or the...

  12. Condition to be fulfill for nuclear energetics to be competitive on the base of 16. Congress WEC in Tokyo, Seminar NG NPP`96 in Warsaw and Conference ENS TOPNUX`96 in Paris; Warunki ekonomicznej konkurencyjnosci energetyki jadrowej w swietle materialow 16. Kongresu WEC w Tokio, Seminarium NG NPP`96 w Warszawie oraz Konferencji ENS TOPNUX`96 w Paryzu

    Energy Technology Data Exchange (ETDEWEB)

    Marecki, J. [Politechnika Gdanska, Gdansk (Poland)

    1997-12-31

    The state and developing trends of nuclear energetics have been presented for the period 1970-1990. The economical analysis taking into account actual prices and predicted for near future has been done. The investment and exploitation costs have been discussed and compared with classic methods of energy generation. It has been concluded that nuclear energetics will be one of the most promising options for future development of world energetics when all aspects (economic, technical, ecological) of the problem have been taken into consideration. 10 refs, 6 figs, 6 tabs.

  13. (R-(+-α-Lipoic acid protected NG108-15 cells against H2O2-induced cell death through PI3K-Akt/GSK-3β pathway and suppression of NF-κβ-cytokines

    Directory of Open Access Journals (Sweden)

    Kamarudin MNA

    2014-10-01

    Full Text Available Muhamad Noor Alfarizal Kamarudin, Nur Afiqah Mohd Raflee, Sharifah Salwa Syed Hussein, Jia Ye Lo, Hadi Supriady, Habsah Abdul KadirInstitute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, MalaysiaAbstract: Alpha-lipoic acid, a potent antioxidant with multifarious pharmacological benefits has been reported to be neuroprotective in several neuronal models and used to treat neurological disorders such as Alzheimer’s disease. Nonetheless, conclusive mechanisms of alpha-lipoic acid for its protective effects particularly in NG108-15 cells have never been investigated. In this study, the intricate neuroprotective molecular mechanisms by (R-(+-alpha-lipoic acid (R-LA against H2O2-induced cell death in an in vitro model of neurodegeneration were elucidated. Pretreatment with R-LA (2 hours significantly increased NG108-15 cell viability as compared to H2O2-treated cells and mitigated the induction of apoptosis as evidenced by Hoechst 33342/propidium iodide staining. R-LA (12.5–50 µM aggrandized the reduced glutathione over glutathione disulfide ratio followed by a reduction in the intracellular reactive oxygen species level and an increase in mitochondrial membrane potential following H2O2 exposure. Moreover, pretreatment with R-LA stimulated the activation of PI3K-Akt through mTORC1 and mTORC2 components (mTOR, rictor and raptor and production of antiinflammatory cytokine, IL-10 which led to the inactivation of glycogen synthase kinase-3β (GSK-3β and reduction of both Bax/Bcl2 and Bax/Bcl-xL ratios, accompanied by inhibition of the cleaved caspase-3. Additionally, this observation was preceded by the suppression of NF-κβ p65 translocation and production of proinflammatory cytokines (IL-6 and TNF-α. The current findings accentuate new mechanistic insight of R-LA against apoptogenic and brain inflammatory factors in a neuronal model. These results further advocate the therapeutic potential of R-LA for

  14. Ciclo biológico de Paraibatrema inesperata n.g., n.sp. (Trematoda, Paramphistomidae, a partir de metacercárias desenvolvidas em Biomphalaria tenagophila (D'Orbigny, 1835 (Mollusca, Planorbidae

    Directory of Open Access Journals (Sweden)

    Marlene Tiduko Ueta

    1981-03-01

    Full Text Available Em exemplares de B. tenagophila, capturados em ambiente natural, foram encontradas paranfistomocercárias de precoce encistamento. Procedeu-se à infecção, per os, de animais de biotério; em camundongos e ratos, verificou-se o desenvolviemnto de trematóide paranfistomídeo desconhecido e que, por suas características, não se enquadra nos g~eneros conhecidos de Paramphistomidae de mamíferos. Para o parasito em apreço é proposta a denominação Paraibatema inesperata n.g, n.sp.. A partir de miracídios, oriundos de ovos eliminados pelos roedores em experiência, procurou-se infectar B. tenagophila e B. glabrata, criadas no moluscário. Verificou-se ser ativa a penetração dos miracídios no tecido do molusco, sendo freqüente a formação de tumorações nas antenas. A evolução experimental no hospedeiro intermediário apresenta-se lenta; ao fim de 40 dias de pós-infecção, não se observou a formação de esporocistos, rédias e cercárias.The new genus and new species of paramphistomid trematode, Paraibatrema inesperata n.g, n.sp., are proposed. The parasite was originally found as a quickly encysting paramphistome cercaria from specimens of the planorbid snail Biomphalaria tenagophila caught in natural habitats. Experimental infection resulted from intubation of metacercariae into the stomach of white mice and rats, which passed eggs in the feces 20 days later. Specimens of B. tenagophila and B. glabrata have been exposed to miracidia from eggs passed by mice. Penetration of the molluscan tegument was observed, but it seems that the larval development of the parasite is slow, inasmuch as no rediae and cercariae have been obtained as yet.

  15. Impact of Comorbidity, Race, and Marital Status in Men Referred for Prostate Biopsy with PSA >20 ng/mL: A Pilot Study in High-Risk Patients.

    Science.gov (United States)

    Klaassen, Zachary; Muller, Roberto; Li, Qiang; Tatem, Alexander J; King, Sherita A; Freedland, Stephen J; Madi, Rabii; Terris, Martha K; Moses, Kelvin A

    2014-01-01

    Objective. To assess the impact of comorbidity, race, and marital status on overall survival (OS) among men presenting for prostate biopsy with PSA >20 ng/mL. Methods. Data were reviewed from 2000 to 2012 and 78 patients were included in the cohort. We analyzed predictors of OS using a Cox proportional hazards model and the association between Charlson Comorbidity Index (CCI) score and PCa diagnosis or high-grade cancer using logistic regression and multinomial regression models, respectively. Results. The median age of patients was 62.5 (IQR 57-73) years. Median CCI was 3 (IQR 2-4), 69% of patients were African American men, 56% of patients were married, and 85% of patients had a positive biopsy. CCI (HR 1.52, 95% CI 1.19, 1.94), PSA (HR 1.62, 95% CI 1.09, 2.42), and Gleason sum (HR 2.04, 95% CI 1.17, 3.56) were associated with OS. CCI was associated with Gleason sum 7 (OR 4.06, 95% CI 1.04, 15.89) and Gleason sum 8-10 (OR 4.52, 95% CI 1.16, 17.54) PCa. Conclusions. CCI is an independent predictor of high-grade disease and worse OS among men with PCa. Race and marital status were not significantly associated with survival in this cohort. Patient comorbidity is an important component of determining the optimal approach to management of prostate cancer. PMID:27355056

  16. Frammistöðukvíði í tengslum við söng og tónlistarflutning : helstu ráð og meðferðarform

    OpenAIRE

    Elísabet Ólafsdóttir 1977

    2016-01-01

    Frammistöðukvíða þekkja líklega flestir sem hafa talað, sungið eða flutt tónlist fyrir framan áheyrendur. Tilgangur þessarar ritgerðar er að taka saman rannsóknir um helstu ráð og meðferðarform við frammistöðukvíða og meta aðferðafræði þeirra og niðurstöður á gagnrýninn hátt í þeirri von að sú samantekt megi nýtast í tónlistar- og söngkennslu á Íslandi. Við leit í rafrænum gagnagrunnum fundust alls 13 ritrýndar rannsóknargreinar varðandi frammistöðukvíða sem tengdust tónlist og/eða söng með b...

  17. The Politics of Frontier Mining: Local Chieftains, Chinese Miners, and Upland Society in the Nông Văn Vân Uprising in the Sino-Vietnamese Border Area, 1833–1835

    Directory of Open Access Journals (Sweden)

    Vũ Đường Luân

    2014-06-01

    Full Text Available This article, part of a longer study of the history of mining in Vietnam, argues that the Nông Văn Vân uprising (1833–1835 in the northern uplands of Vietnam brings into relief the importance of mining in the Vietnamese economy of the nineteenth century. It also highlights the consequences of Emperor Minh Mệnh’s dual agenda of extracting more tax revenues from mining operations and expanding the reach of the state by replacing hereditary tribal chieftains with imperial bureaucrats. While the uprising was quelled, the imperial agenda could not be fully realized in the face of local opposition and declining revenue from mining. The uprising reflected the multiethnic nature of border society, composed as it was of Vietnamese, local minority populations, and a significant number of Chinese mine owners, workers, and providers of goods and services to the mining towns. In ordinary times, the border was regularly flouted as kinship relations, trading networks, and ethnic affinity transcended allegiance to either the Qing or the Nguyễn in this borderland. Although the uprising was formally contained within the Vietnamese territory, rebels were able to seek refuge and recruit new adherents in China. And while the Nguyễn court was eventually able to subdue the rebels, its centralizing policies and attempts at extracting more revenues from mining were ultimately unsuccessful in the face of reemerging local and transborder forces.

  18. The impact of heavy metals from environmental tobacco smoke on indoor air quality as determined by Compton suppression neutron activation analysis.

    Science.gov (United States)

    Landsberger, S; Wu, D

    1995-12-01

    The method of instrumental neutron activation analysis (NAA) has been improved for air filter samples in the determination of low level heavy metals in indoor air. By using the techniques of epithermal neutron irradiation in conjunction with Compton suppression, the detection limits of cadmium, arsenic and antimony measurements have been dramatically reduced to 2 ng for Cd, 0.2 ng for As, and 0.03 ng for Sb. The determination of these heavy metals in particulate material generated from cigarette smoking in indoor environments has been conducted. Other elements, Br, Cl, Na, K, Zn were also found at elevated levels. PMID:8560226

  19. 3-Methylmethcathinone-Interpretation of Blood Concentrations Based on Analysis of 95 Cases.

    Science.gov (United States)

    Adamowicz, Piotr; Gieroń, Joanna; Gil, Dominika; Lechowicz, Wojciech; Skulska, Agnieszka; Tokarczyk, Bogdan

    2016-05-01

    3-Methylmethcathinone (3-MMC) has been one of the most popular new psychoactive substances (NPS) in Poland in recent years. 3-MMC was found in blood in 95 cases sent to the Institute of Forensic Research (IFR) during the two and a half year period, from 2013 to half of 2015. 3-MMC was determined in 13 and 48 cases in 2013 and 2014 year-round casework, respectively, while only in the first half of 2015 year it was present in 34 cases. In most cases, 3-MMC was detected together with other novel psychoactive substances and conventional drugs. Blood analyses for 3-MMC were carried out using liquid chromatography-tandem mass spectrometry (LC-MS-MS). The concentrations of 3-MMC in all 95 cases were in the range from traces (accidents: <1-29 ng/mL; drug possession: 2-408 ng/mL; intoxication: <1-1600 ng/mL and other: <1-61 ng/mL. The parameters of the developed method such as the LOD (0.02 ng/mL) and LOQ (1 ng/mL) demonstrate that the method is well suited for the analysis of blood samples for 3-MMC and covers the range of typical blood concentrations. PMID:26989222

  20. 3c/4e [small sigma, Greek, circumflex]-type long-bonding competes with ω-bonding in noble-gas hydrides HNgY (Ng = He, Ne, Ar, Kr, Xe, Rn; Y = F, Cl, Br, I): a NBO/NRT perspective.

    Science.gov (United States)

    Zhang, Guiqiu; Li, Hong; Weinhold, Frank; Chen, Dezhan

    2016-03-01

    Noble-gas hydrides HNgY are frequently described as a single ionic form (H-Ng)(+)Y(-). We apply natural bond orbital (NBO) and natural resonance theory (NRT) analyses to a series of noble-gas hydrides HNgY (Ng = He, Ne, Ar, Kr, Xe, Rn; Y = F, Cl, Br, I) to gain quantitative insight into the resonance bonding of these hypervalent molecules. We find that each of the studied species should be better represented as a resonance hybrid of three leading resonance structures, namely, H-Ng(+ -):Y (I), H:(- +)Ng-Y (II), and H^Y (III), in which the "ω-bonded" structures I and II arise from the complementary donor-acceptor interactions nY → σ*HNg and nH → σ*NgY, while the "long-bond" ([small sigma, Greek, circumflex]-type) structure III arises from the nNg → [small sigma, Greek, circumflex]*HY/[small sigma, Greek, circumflex]HY interaction. The bonding for all of the studied molecules can be well described in terms of the continuously variable resonance weightings of 3c/4e ω-bonding and [small sigma, Greek, circumflex]-type long-bonding motifs. Furthermore, we find that the calculated bond orders satisfy a generalized form of "conservation of bond order" that incorporates both ω-bonding and long-bonding contributions [viz., (bHNg + bNgY) + bHY = bω-bonding + blong-bonding = 1]. Such "conservation" throughout the title series implies a competitive relationship between ω-bonding and [small sigma, Greek, circumflex]-type long-bonding, whose variations are found to depend in a chemically reasonable manner on the electronegativity of Y and the outer valence-shell character of the central Ng atom. The calculated bond orders are also found to exhibit chemically reasonable correlations with bond lengths, vibrational frequencies, and bond dissociation energies, in accord with Badger's rule and related empirical relationships. Overall, the results provide electronic principles and chemical insight that may prove useful in the rational design of noble-gas hydrides of

  1. Analysis of Some Egyptian Cosmetic Samples by Fast Neutron Activation Analysis

    CERN Document Server

    Medhat, M E; Fayez-Hassan, M

    2001-01-01

    A description of D-T neutron generator (NG) is presented. This generator can be used for fast neutron activation analysis applied to determine some selected elements, especially light elements, in different materials. In our work, the concentration of the elements Na, Mg, Al, Si, K, Cl, Ca and Fe, were determined in two domestic brands of face powder by using 14 MeV neutron activation analysis.

  2. Re-imahinasyon ng Ina sa Panitikan ng Kababaihan: Isang Imbestigasyon sa Ideolohiyang Maternal sa Panitikan

    Directory of Open Access Journals (Sweden)

    Rosario Torres-Yu

    2000-06-01

    Full Text Available The narratives generated by my earlier study of 20 women writers from three generations were a rich source of data and insights on many issues on gender and writing, such as motherhood and its representation in literature. It is their ideas on motherhood, which led me to ask how some of them represent motherhood in their works and what these representations say about their positions on the dominant maternal ideology in Philippine culture.Another impetus came from Delia Aguilar's study on Filipino housewives and the politics of gender. This study reveals that " . . . in the private space of the family as the major mechanism for the reproduction of social relations, women are at once validated and oppressed, a process that is powerfully mediated by the maternal ideology of which women are the foremost proponents." This maternal ideology shapes Filipino women's idea of motherhood that sees devotion to children, self-sacrifice, and care giving as the essence of motherhood, allowing them to embrace oppression and subordination. In this context, the interrogation of maternal ideology in Philippine literature is important to the overall feminist movement. Women writers who have freed themselves from the mystifying notions of motherhood may have been inspired to represent women in their works differently.This paper clearly validates such assumption. Writers like Liwayway Arceo, Genoveva Edroza-Matute, Fanny Garcia, Lualhati Bautista, Lilia Quindoza-Santiago, and Luna Sicat-Cleto, as this reading of some of their works reveals, challenge the dominant maternal ideology by re-imagining motherhood. Without wholly rejecting the traditional notion of motherhood (with the exception of Luna Sicat-Cleto who questions the so called "essence" of motherhood, these writers interrogate the idealization of motherhood, question some of the fundamental assumptions of the dominant maternal ideology and represent women as mothers who are nurturing and caring just like the typical image of a Filipino mother, but who are not happy with confinement in the private domestic space. This radical representation of motherhood is, therefore, a welcome development in the present development of feminist discourse in the Philippines.

  3. Pagsasatinig ng Pagkabata: Ang Varayti ng Wika sa mga Premyadong Kuwentong Pambata

    OpenAIRE

    Wennielyn F. Fajilan

    2014-01-01

    Writing for children is a way of cultivating their minds. As a special kind of communication, children’s literature entails the use of a language that caters to the needs and skills of the child reader. The study aims to analyse the variety of language used for children in the narratives of eight prize winning children’s stories—awarded the Palanca Award and Philippine Board of Books for Young People (PBBY) prize from 1995-2009—with themes on motherhood or fatherhood. The study found that con...

  4. Pagsasatinig ng Pagkabata: Ang Varayti ng Wika sa mga Premyadong Kuwentong Pambata

    Directory of Open Access Journals (Sweden)

    Wennielyn F. Fajilan

    2014-12-01

    Full Text Available Writing for children is a way of cultivating their minds. As a special kind of communication, children’s literature entails the use of a language that caters to the needs and skills of the child reader. The study aims to analyse the variety of language used for children in the narratives of eight prize winning children’s stories—awarded the Palanca Award and Philippine Board of Books for Young People (PBBY prize from 1995-2009—with themes on motherhood or fatherhood. The study found that concepts of motherhood such as that of the traditional homemaker, the OFW, the single mother, and lesbian mothers; concepts of fatherhood like the OFW father, environmentalist and revolutionary parents characterize the selected stories. Through an examination of the dialogues, actions, and descriptions written for the child reader, the paper illustrates how narratives of the self, orality, and intelligence characterize the child protagonist. Hence, an examination of the contemporary children’s stories’ language variety is also an investigation of the prevailing representation of children in Philippine language, literature, and society.

  5. Ang Dumaracol ng mga Kalamianen Tagbanua ng Hilagang Palawan: Isang Panimulang Paglalahad

    Directory of Open Access Journals (Sweden)

    Eulalio R. Guieb III

    2014-12-01

    Full Text Available This study is the first documentation of the long narrative song dumaracol of the Kalamianen Tagbanua of Northern Palawan on the islands bordering the towns of Taytay, El Nido, and Linapacan. The essay is the first in a multi-stage research that collects, records, documents, transcribes, translates into Filipino, and examines the said genre. In the song, Dumaracol is the name of the hero of the Kalamianen Tagbanua. The song narrates the life, love, and struggles of Dumaracol in establishing and maintaining their community against the slave-raiding Moro. The essay discusses the different versions of the song, but highlights that by Blas Juan, one of the contemporary leaders of the Kalamianen Tagbanua of Pical Island in Linapacan. The essay also presents the processes of the research documentation of the song. It identifies and brings to the fore various issues in the preliminary understanding of dumaracol.

  6. The Case Method: "I’m not sure ..." Faci ng Barriers to Implementi ng the Method

    Directory of Open Access Journals (Sweden)

    Roberta Dias Campos

    2014-03-01

    extension courses for teachers. It presents the dilemma experienced by Cosmo, a young professor recently hired by the iesg (Institute of Higher Education in Management, who wishes to adopt the case method as a teaching practice within an institutional setting and with distinct and heterogeneous didactic experiences. It also presents the views of different stakeholders in the institution, discussing the challenge to accommodate its teaching strategy with its professionals’ individual and cultural daily practice. The case intends to foster reflection on the barriers and actions required for institutional case method adoption, seen from three of the organization’s levels: the faculty, the student body and the institutional structure as a whole.

  7. Ang Dalumat ng Panahon at Espasyo sa Mga Traki ng Dulangan Manobo

    Directory of Open Access Journals (Sweden)

    Rosario Cruz-Lucero

    2002-06-01

    Full Text Available This paper introduces the duyuy nga traki “narrative chants” of the Dulangan Manobo, one of the least-documented subtribes of the large Manobo tribe inhabiting most of Mindanao. It attempts an interpretation of their concept of time and space as expressed, implicitly or explicitly, in these traki. The empirical bases for these interpretations are cultural, political and historical data, which are woven into theanalyses. Contemporary data include the tribe’s attempts to assert their Ancestral Domain Claims. Their creation myth, trickster tale, origin stories, etiological legends about natural formations, etc. are interpreted in the light of their economic system and the shifts from one occupational practice to another; historical experiences, such as the coming of outsiders, viz., the Muslims, Americans and homesteaders; and the politics of their location.The narrative chants were recorded in the original Manobo while an interpreter translated these line-by-line into Hiligaynon, which I then translated in Filipino. The narrative chants presented in this paper are the translations into Filipino. This research was conducted in the municipality of Kulaman and the village of Todog, both in the province of Sultan Kudarat. Two weeklong visits were made by this writer to these areas in October 2002 and May 2003, primarily to record the duyuy nga traki performed by Baha Lambay Beliyan, and to interact informally with the Dulangans.

  8. Dance Dance Revolution: Usapin ng Laro at Sayaw sa Panahon ng Globalisasyon

    Directory of Open Access Journals (Sweden)

    Jema M. Pamintuan

    2001-06-01

    Full Text Available The Dance Dance Revolution (DDR Machine is becoming a favorite game among the youth and even the not-so-young. While much has been written on the mechanics and popularity of the game, this essay aims to probe into the DDR phenomenon as a parallel tool and response to the changes brought about by technology and the age of globalization. The individual's relationship with the machine, with his audience, and with the larger society will be documented and juxtaposed with the activities of a global village. The cultural implications of the DDR will also be discussed, especially how the game itself revolutionized our traditional concept of game and dance.

  9. INVESTIGATION OF THE METHODS OF COPİNG WİTH STRESS OF PHYSİCAL EDUCATION TEACHERS THAT WORK IN PRİMARY AND SECONDARY EDUCATİON SCHOOL (EXAMPLE OF AYDIN

    Directory of Open Access Journals (Sweden)

    Hüseyin KIRIMOĞLU

    2011-08-01

    Full Text Available Open Journal Systems Journal Help User You are logged in as...hazarserkan •My Journals•My Profile•Log OutLanguage English Türkiye Türkçesi Journal Content Search All Authors Title Abstract Index terms Full Text Browse •By Issue•By Author•By Title•Other JournalsFont Size Make font size smaller Make font size default Make font size larger HomeAboutUser HomeSearchCurrentArchivesAnnouncementsHome > Vol 5, No 2 (2011 > KIRIMOĞLU INVESTIGATION OF THE METHODS OF COPİNG WİTH STRESS OF PHYSİCAL EDUCATION TEACHERS THAT WORK IN PRİMARY AND SECONDARY EDUCATİON SCHOOL (EXAMPLE OF AYDINHüseyin KIRIMOĞLU, Yunus YILDIRIM, Ali TEMİZAbstractThis investigation was performed for discover of whether the methods of coping with stres of physical educatıon teachers that work in Aydın change or not.96 physical education teachers (28 women and 68 men that work in primary and secondary education school in Aydın (country, town, village took part in thıs investigation. For detection of methods of coping with stress; personal information form improved by investigator and ‘ scale of coping with stress ’ improved by original (Özbay, 1993 were used. SPSS 16.0 statistic package programme was used for evaluate of data and find the calculated data. For trial of normality, t test (whose group number is 2 and One – Way ANOVA test (whose group number is more than 2 were used within parametric tests. For variance homology; Tukey HSD test was used within Post Hoc Multiple Comparisions tests. In thıs study ratio of fault was assessed 0.05. When we compared the participants’s methods of coping with stress by some variable factors; statistical significiant differences were found in point of gender; asking for help from others, in point of civil status; taking shelter for religious, in multiply comprision for mission place (country, town, village; taking shelter for religious, active planning, escape-abstraction (biochemical. We can say that there is

  10. Improved survival in MDS patients receiving iron chelation therapy - a matched pair analysis of 188 patients from the Düsseldorf MDS registry.

    Science.gov (United States)

    Neukirchen, Judith; Fox, Frank; Kündgen, Andrea; Nachtkamp, Kathrin; Strupp, Corinna; Haas, Rainer; Germing, Ulrich; Gattermann, Norbert

    2012-08-01

    MDS patients are prone to develop transfusional iron overload. Iron overload may partly explain why transfusion dependency is associated with a decreased likelihood of survival. Our matched-pair analysis included 94 patients on long-term chelation therapy and 94 matched patients without it. All patients had iron overload, defined as serum ferritin (SF) above 1000 ng/ml or a history of multiple transfusions and SF ≥ 500 ng/ml. Median SF was 1954 ng/ml in chelated and 875 ng/ml in non-chelated patients. The difference in median survival (74 vs. 49 months, respectively; p=0.002) supports the idea that iron chelation therapy is beneficial for MDS patients. PMID:22564985

  11. Draped heterogeneity, forced uniformity: when agro-environmental policies drive family development: The U Minh Thượng forest reserve, (Mekong delta, Vietnam) Hétérogénéité cachée, uniformité forcée : quand les politiques agro-environnementales guident le développement familial – Cas de la réserve forestière de U Minh Thượng (delta du Mékong, Vietnam) Heterogeneidad oculta, uniformidad forzada: cuando las políticas agro-ambientales impulsan el desarrollo familiar. La reserva del bosque de U Minh Thượng (delta del Mekong, Vietnam)

    OpenAIRE

    Mehdi Saqalli; Mireille Dosso

    2011-01-01

    The U Minh Thượng natural reserve was created during the 1990s with a surrounding buffer zone where 3,500 households have been settled between 1992 and 1995, each household on a lot of 4 hectares. From a social point of view, the settlers were selected as 'poor people' or war veterans. A social discrimination was slowly built in favour of the latter. Despite an apparent homogeneity, the environment shows slight variations which has huge effects on the potential of the lots. From 1992 to 1998,...

  12. Comparison between charged aerosol detection and light scattering detection for the analysis of Leishmania membrane phospholipids.

    Science.gov (United States)

    Ramos, R Godoy; Libong, D; Rakotomanga, M; Gaudin, K; Loiseau, P M; Chaminade, P

    2008-10-31

    The performance of charged aerosol detection (CAD) was compared to evaporative light scattering detection (ELSD) for the analysis of Leishmania membrane phospholipid (PL) classes by NP-HPLC. In both methods, a PVA-Sil column was used for the determination of the major Leishmania membrane PLs, phosphatidic acid, phosphatidylglycerol, cardiolipin, phosphatidylinositol, phosphatidylethathanolamine, phosphatidylserine, lysophosphatidylethathanolamine, phosphatidylcholine, sphingomyelin and lysophosphatidylcholine in the same analysis. Although the response of both detection methods can be fitted to a power function, CAD response can also be described by a linear model with determination coefficients (R(2)) ranging from 0.993 to 0.998 for an injected mass of 30 ng to 20.00 microg. CAD appeared to be directly proportional when a restricted range was used and it was found to be more sensitive at lowest mass range than ELSD. With HPLC-ELSD the limits of detection (LODs) were between 71 and 1195 ng and the limits of quantification (LOQs) were between 215 and 3622 ng. With HPLC-CAD, the LODs were between 15 and 249 ng whereas the limits of quantification (LOQs) were between 45 and 707 ng. The accuracy of the methods ranged from 62.8 to 115.8% and from 58.4 to 110.5% for ELSD and CAD, respectively. The HPLC-CAD method is suitable to assess the influence of miltefosine on the composition of Leishmania membrane phospholipids. PMID:18823632

  13. Ruh Adam Romanında Kahramanın Sonsuz Yolculuğu: Yasak Aşk’ın Döngüsü The Endless Journey of Hero in Ruh Adam Novel: Cycle of Forbidden Love

    Directory of Open Access Journals (Sweden)

    Gürhan ÇOPUR

    2012-09-01

    şadığı olumsuzluklar neticesinde içerisine düştüğü bunalım ile başlayan olaylar, onun yaşamının dikey eksende çizilen sonuna doğru başkişiyi kendi iradesi dışında sürükleyecektir. Romanda, başkişi tarafından yaşamın anlamlandırılamayan/yönlendirilemeyen kodları yaşamını çevreler ve onu bir yeniden doğuş sanrısıyla kuşatır. Yeniden doğuşunu klasik bir reenkarnasyon olarak değil simgesel düzlemde önceden hazırlanmış bir döngünün merkezinde yaşayan Selim Pusat, mücadele ettikçe içerisinde kaybolacağı bunalımın derinliklerinde yaşamının en kutsal değerlerinin benliğini sıkıştırmasıyla geçmiş ve bugünün tuğlaları ile örülen bir labirentin duvarları arasında sıkışır. Selim Pusat, içerisinden çıkamadığı bu „labirent‟te yakınlarının şahsında vücut bulan kendilik değerleri ile hesaplaşmasından sembolik anlamda yok oluşuna kadar uzanan çizgide ele alınır.Bu çalışmada, geçmişinin döngüselliğine karşı çaresiz kalan ve yazgısını karşılıksız bir aşkın lanetinde yokluğa karışmakla yaşayan başkişi Selim Pusat‟ın çiğnediği benlik değerleri tarafından yargılanışı ele alınacaktır. Yolculuk, temel anlamı dışında bireyin yaşantısında kader ve kendi adımlarıyla oluşturduğu yaşanmışlıklar bütününün adıdır. Bu yolculukta kurulan bütün aşamalar başkişinin özsel anlamdaki değişimleri, dönüşümleri ve psikolojik durumları bir bütün olarak değerlendirilir. Selim Pusat‟ın yaşamı da arketipsel anlamda yolculuk imgesi üzerine değerlendirildiğinde ortaya bir aşk romanından ötesi çıkar. İzlenecek yolda Joseph Campbell‟in “Kahramanın Sonsuz Yolculuğu” adlı eserinde ele alınan arketipsel dönüşüm aşamaları merkeze alınacaktır.

  14. Sjuksköterskans dilemma inom psykiatrisk slutenvård - En balansgång mellan gott och ont : Sjuksköterskors erfarenheter av att lindra patienters lidande orsakat av tvångsåtgärder

    OpenAIRE

    Källbom, Annika; Mitts-Boduroglu, Maria

    2014-01-01

    Title: The nurse's dilemma in psychiatric inpatient care - a balancing act between good and evil Objective: To describe the nurse's experience of relieving patient suffering caused by coercive measures on a closed psychiatric ward . Frame of reference : The results are discussed in light of Eriksson´s theory of suffering and caring relationships Method: A qualitative analysis based on focus group discussions Results: The analysis resulted in six categories, to protect the patient , to involve...

  15. Budgetlös styrning : Har Wallanders budgetlösa styrning stått sig oförändrad i närmare 40 år ochhar den varit ett vinnande koncept till framgång?

    OpenAIRE

    Jotorp, Sanne; Strandh, Emelie; Wilhelmsson, Katrin

    2010-01-01

    Datum: 2010-06-03 Nivå: Magisteruppsats i företagsekonomi, 15 hp Författare: Sanne Jotorp, Emelie Strandh och Katrin Wilhelmsson Handledare: Esbjörn Segelod Titel: Budgetlös styrning - Har Wallanders budgetlösa styrning stått sig oförändrad inärmare 40 år och har den varit ett vinnande koncept till framgång? Syfte: Syftet med uppsatsen är att undersöka huruvida Handelsbankens budgetlösa styrning utvecklats sedan införandet för nästan 40 år sedan samt att studera om bankens framgång grundar si...

  16. Differentiation of NG108-15 cells induced by the combined presence of dbcAMP and dexamethasone brings about the expression of N and P/Q types of calcium channels and the inhibitory influence of muscarinic receptors on calcium influx

    Czech Academy of Sciences Publication Activity Database

    Doležal, Vladimír; Lisá, Věra; Diebler, M. F.; Kašparová, Jana; Tuček, Stanislav

    2001-01-01

    Roč. 910, 1-2 (2001), s. 134-141. ISSN 0006-8993 R&D Projects: GA ČR GA305/98/0469; GA ČR GA305/01/0283; GA MZd NF5183 Institutional research plan: CEZ:AV0Z5011922 Keywords : NG108-15 cell line * cAMP * calcium channels Subject RIV: ED - Physiology Impact factor: 2.489, year: 2001

  17. A novel preconcentration technique for the PIXE analysis of water

    International Nuclear Information System (INIS)

    The potential of using dried algae as a novel preconcentration technique for the analysis of water samples by PIXE was examined. The algae cells were found to contain significant levels of P and S, indicative of phosphorous- and sulfur-containing groups on the cell wall or inside the algae cells which may serve as potential binding sites for metal ions. When C. vulgaris was used on mixed metal solutions, linear responses were observed for Ag+, Ba2+, and Cd2+ in the concentration range from 10 ng/g to 1 μg/g; for Cu2+ and Pb2+ from 10 ng/g to 5 μg/g; and for Hg2+ from 10 ng/g to 10 μg/g. When S. bacillaris was used, linear responses were observed from 10 ng/g up to 10 μg/g for all of the metal cations investigated. The PIXE results demonstrated that metal binding at low concentrations involves replacement of sodium on the cell wall and that at high concentrations magnesium was also replaced. Competitive binding studies indicate that the metal ions, Ag+, Ba2+, Cd2+, Cu2+, and Pb2+, share common binding sites with binding efficiencies varying in the sequence of Pb2+>Cu2+>Ag2+>Cd2+>Ba2+. The binding of Hg2+ involved a different binding site with an increase in binding efficiency in the presence of Ag+. (orig.)

  18. Environmental and occupational exposure to benzene by analysis of breath and blood.

    Science.gov (United States)

    Perbellini, L; Faccini, G B; Pasini, F; Cazzoli, F; Pistoia, S; Rosellini, R; Valsecchi, M; Brugnone, F

    1988-05-01

    Benzene exposure of chemical workers was studied, during the entire workshift, by continuous monitoring of workplace benzene concentration, and 16 hours after the end of the workshift by the measurement of alveolar and blood benzene concentrations and excretion of urinary phenol. Exposure of hospital staff was studied by measuring benzene concentrations in the alveolar and blood samples collected during the hospital workshift. Instantaneous environmental air samples were also collected, at the moment of the biological sampling, for all the subjects tested. A group of 34 chemical workers showed an eight hour exposure to benzene, as a geometric mean, of 1.12 micrograms/l which corresponded, 16 hours after the end of the workshift, to a geometric mean benzene concentration of 70 ng/l in the alveolar air and 597 ng/l in the blood. Another group of 27 chemical workers (group A) turned out to be exposed to an indeterminable eight hour exposure to benzene that corresponded, the morning after, to a geometric mean benzene concentration of 28 ng/l in the alveolar air and 256 ng/l in the blood. The group of hospital staff (group B) had a benzene concentration of 14 ng/l in the alveolar air and 269 ng/l in the blood. Instantaneous environmental samples showed that in the infirmaries the geometric mean benzene concentration was 58 ng/l during the examination of the 34 chemical workers, 36 ng/l during the examination of the 27 chemical workers (group A), and 5 ng/l during the examination of the 19 subjects of the hospital staff (group B). Statistical analysis showed that the alveolar and blood benzene concentrations in the 34 workers exposed to 1.12 microgram/l of benzene differed significantly from those in groups A and B. It was found, moreover, that the alveolar and blood benzene concentrations were higher in the smokers in groups A and B but not in the smokers in the group of 34 chemical workers. The slope of the linear correlation between the alveolar and the instantaneous

  19. Samhällsvård och välfärdsresurser : En studie av skolgång, fritid och kamratrelationer bland unga i familjehem och institutioner

    OpenAIRE

    Lagerlöf, Hélène

    2012-01-01

    The dissertation analyses access to welfare resources within the areas of schooling, leisure and peer relations for youth in out of home care. The study was conducted in three counties in mid Sweden and is a replication of the recurrent Swedish surveys of living condition of children in general populations. By using the same design, children aged 13–18 (n=272) in foster care and residential care were approached. Throughout the analysis results are compared with conditions for peers living at ...

  20. Methods for analysis of citrinin in human blood and urine.

    Science.gov (United States)

    Blaszkewicz, Meinolf; Muñoz, Katherine; Degen, Gisela H

    2013-06-01

    Citrinin (CIT), produced by several Penicillium, Aspergillus, and Monascus species, has been detected as contaminant in feeds, grains, and other food commodities. CIT can co-occur with ochratoxin A (OTA), a mycotoxin also known for its nephrotoxicity, and this raises concern regarding possible combined effects. But, in contrast to OTA, data on CIT contamination in foods for human consumption are scarce, and CIT biomonitoring has not been conducted so far due a lack of suitable methods for human specimen. Thus, it was the aim of the present study to develop sensitive methods for the analysis of CIT in human blood and urine to investigate human exposure. To this end, we assessed different methods of sample preparation and instrumental analysis for these matrices. Clean-up of blood plasma by protein precipitation followed by LC-MS/MS-based analysis allowed robust detection of CIT (LOD 0.07 ng/mL, LOQ 0.15 ng/mL). For urine, sample clean-up by an immunoaffinity column (CitriTest(®)) proved to be clearly superior to SPE with RP(18) material for subsequent analysis by LC-MS/MS. For CIT and its metabolite dihydrocitrinone (HO-CIT), the LOD and LOQ determined by external calibration curves in matrix were 0.02 and 0.05 ng/mL for CIT, and those for HO-CIT were 0.05 and 0.1 ng/mL urine. The newly developed method was applied in a small pilot study: CIT was present in all plasma samples from 8 German adults, at concentrations ranging from 0.11 to 0.26 ng/mL. The molar (nM) concentrations of CIT are similar to those measured for OTA in these samples as a result of dietary mycotoxin intake. CIT was detected in 8/10 urines (from 4 adults and 6 infants) in a range of 0.16-0.79 ng/mL, and HO-CIT was present in 5/10 samples at similar concentrations. Thus, CIT is excreted in urine as parent compound and also as metabolite. These first results in humans point to the need for further studies on CIT exposure. PMID:23354378

  1. Condition to be fulfill for nuclear energetics to be competitive on the base of 16. Congress WEC in Tokyo, Seminar NG NPP'96 in Warsaw and Conference ENS TOPNUX'96 in Paris

    International Nuclear Information System (INIS)

    The state and developing trends of nuclear energetics have been presented for the period 1970-1990. The economical analysis taking into account actual prices and predicted for near future has been done. The investment and exploitation costs have been discussed and compared with classic methods of energy generation. It has been concluded that nuclear energetics will be one of the most promising options for future development of world energetics when all aspects (economic, technical, ecological) of the problem have been taken into consideration

  2. THE GRADE OF TEXT PRESENTİNG, TERMS, TEXT ANALYSİS / METİN İNCELEMESİ AŞAMALARI, TERİMLERİ VE BUNLARDAN BİRİ: METİN TAHLİLİ

    OpenAIRE

    Prof. Dr. Mine MENGİ

    2007-01-01

    To serve a manuscript belongs to Classical Turkish Literature to a comtemporary researcher or reader, requires the text to be written in Modern Turkish scripture. Text critic is a study that based on a lot of studies from the author to book. These studies can be listed as determine of text, critic of text, corretion of text, formation and presentation of text. But, after the formation or publication, text commentary and text analysis is necessary for the sake of introducing the text. In this ...

  3. STU DY ON THE TEACHING PATTERN OF BODY BUILDI NG IN ORDINARY HIGHER LEARNING INSTITUT IONS%对普通高校健身课教学模式的探讨

    Institute of Scientific and Technical Information of China (English)

    周瑞琪

    2001-01-01

    从体育健身教育观点出发,对大学体育健身课教学模式研究的实践效果作一分析与探讨。旨在促进和推动高校健身教育体系的深入研究和有效实施。%Starting from body building education, the paper made an analysis and study of the results achieved in body huilding teaching practice. The aim is to push forward the teaching and research of body building in colleg e students′ PE class and carry out the pattern more efficiently.

  4. Analysis of perfluoroalkyl substances in waters from Germany and Spain.

    Science.gov (United States)

    Llorca, Marta; Farré, Marinella; Picó, Yolanda; Müller, Jutta; Knepper, Thomas P; Barceló, Damià

    2012-08-01

    Water has been identified as one of the main routes of human exposure to perfluoroalkyl substances (PFASs). This work assessed the presence of 21 PFASs along the whole water cycle using a new fast and cost effective analytical method based on an online sample enrichment followed by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). The method was validated for different types of matrices (ultrapure water, tap water and treated wastewater). The quality parameters for the 21 selected compounds presented good limits of detection (LOD) and quantification (LOQ) ranging, in general, from 0.83-10 ng/L to 2.8-50 ng/L, respectively. The method was applied to assess the occurrence of PFASs in 148 water samples of different steps along the whole water cycle, including: mineral bottled water, tap water, river water and treated effluent wastewater, from Germany to Spain. In addition, in order to prove the good performance of the online analytical method, the analysis of PFASs was carried out in parallel using a method based on offline anionic solid phase extraction (SPE) followed by LC-MS/MS. Consistent results were obtained using both approaches. The more frequently found compounds were perfluoroalkyl acids, such as the perfluorobutanoic acid which was in the 54% of the tap water samples investigated with concentrations in the range between 2.4 and 27 ng/L, the perfluoroheptanoic acid (0.23-53 ng/L) and perfluorooctanoic acid (0.16-35 ng/L), and the sulphonate perfluorooctanesulfonate (0.04-258 ng/L) which was the second more frequent compound and also the compound found in with the higher concentration. It should be remarked that the 88% of the samples analyzed presented at least one of the compounds at quantifiable concentrations. In addition, PFASs including short chain compounds were proved to be prevalent in drinking water, and the 50% of the drinking water samples showed quantifiable concentrations of PFASs. It should be said that the great majority of

  5. Thе іmpаct of fіnаncіаl rеportіng of Smаll аnd Mеdіum Sіzеd Entеrprіsеs іn Economіc Dеvеlopmеnt of Bаlkаn Countrіеs

    OpenAIRE

    Artor Nuhiu; Lulzim Krasniqi; Arbër Hoti

    2012-01-01

    Thе іmportаncе of іntеrnаl аnd еxtеrnаl аccountіng аs а sourcе of іnformаtіon for ownеrs аnd mаnаgеrs of smаll еntеrprіsеs аnd thеіr dіffеrеnt stаkеholdеrs іs stеаdіly growіng. It іs of crucіаl іmportаncе thаt thе аccountіng systеms аpplіеd by smаll еntеrprіsеs mееt thеіr аctuаl nееds, provіdіng nеcеssаry іnformаtіon yеt аvoіdіng unjustіfіеd аdmіnіstrаtіvе burdеn. It іs rеcognіsеd thаt аpproprіаtе аccountіng іnformаtіon іs іmportаnt for а succеssful mаnаgеmеnt of а busіnеss whеthеr іt іs lаrg...

  6. Is there a role for 11C-choline PET/CT in the early detection of metastatic disease in surgically treated prostate cancer patients with a mild PSA increase <1.5 ng/ml?

    International Nuclear Information System (INIS)

    PSA were not statistically significant in predicting a positive 11C-choline PET/CT scan both at univariate and multivariate analysis. Instead, PSA kinetics (PSAdt and PSAvel), N status and anti-androgenic therapy at the time of PET scan were statistically significant predictive factors at univariate analysis. Of note, only PSAdt and initial N status were found to be significant and independent predictive factors at multivariate analysis. The mean PSAdt in PET-positive patients was 4.34 months (SD 2.82) while in PET-negative patients it was 13.30 months (SD 9.75) (p = 0.0001). The optimal threshold for PSAdt established by receiver-operating characteristic (ROC) analysis was 7.25 months (AUC 0.85; 95% confidence interval 0.77-0.91) providing 93% sensitivity, 74% specificity, 60% positive predictive value and 96% negative predictive value. In our study, 11C-choline PET/CT was able to detect recurrent disease in 28% of the patients with mild biochemical relapse characterized by very low trigger PSA levels (PSA 11C-choline PET/CT detected distant unexpected metastases in 21% of the patients. At multivariate statistical analysis only PSAdt and node status were shown to be significant and independent predictive factors for positive 11C-choline PET/CT. Therefore, 11C-choline could be suggested to be performed early during initial biochemical relapse in patients presenting with fast PSA kinetics. The early detection of the site of recurrence could lead to a prompt instauration of the most appropriate treatment, i.e. local surgery or radiation treatment vs systemic treatment. In this view, one of the main advantages should be the avoidance of unnecessary local radiotherapy in those patients showing distant metastasis at 11C-choline PET/CT. (orig.)

  7. Determination of chromium, zinc, iron and calcium contents in the raw materials for pastes, using the activation analysis

    International Nuclear Information System (INIS)

    For describing the variation content in trace nutrients as result of food industrialization process, wheat flour, integral eggs and paste made from these raw materials were analyzed using neutron activation analysis technique. The concentration of the elements Cr, Fe, Zn and Ca were found to be roundly ng/g and μg/g. (author). 14 refs., 1 fig., 5 tabs

  8. An Emprıcal Study Intend to Evaluatıon of the Service Presenting By The Groups of Accountancy Profession Towards The Relatıonship Marketıng Percept of Customers

    Directory of Open Access Journals (Sweden)

    Yusuf Kaya

    2014-12-01

    Full Text Available The concept of relationship marketing, which the companies operating in service industry adopted from past to present, but the scientific foundations of which have been laid in the last thirty years, stand out with its failure due to the increasing conditions of competition of traditional sense of marketing. The increasing number of members of accounting profession in Turkey has complicated the conditions of survival in this profession. Accordingly, it has been obligatory for the members of the profession to renew and diversify their existing marketing sense. In this study, it was put forward which factors had an impact on customers to prefer the members of accounting profession, and if these factors had a relationship with relations marketing. In the survey study whih was conducted on 755 business managers that purchased accounting service defined via convenience sampling method in Denizli region, it was questioned why they worked with the members of the profession, why they preferred them and to which points they paid attention in selection. The data were subjected to factor analysis in SPSS 15.0 software and nine basic factors were obtained. The results presented that relationshipmarketing had a significant impact on customers to prefer the members of the profession

  9. İLKÖĞRETİM KURUMU YÖNETİCİLERİNİN LİDERLİK TARZLARI İLE ÖĞRETMENLERİN YAŞADIKLARI YILDIRMA (MOBBİNG ARASINDAKİ İLİŞKİ

    Directory of Open Access Journals (Sweden)

    Elif DAŞÇI

    2015-04-01

    Full Text Available The aim of this research was to determine the relationship between leadership styles of elementary school principals and mobbing that teachers experience. The population of the study consisted of 60 schools and 1301 teachers working in primary schools of Ministry of National Education in 5 Central education districts of Aksaray. The sample set of research were chosen from the population with the multi-stage sampling method. The first stage was stratified sampling and the second stage was simple random sampling. 395 surveys were analyzed in the study. The Multifactor Leadership Questionnaire and Negative Acts Questionnaire were used to collect data. The data were assessed by using Pearson Product-Moment Correlation Coefficient and Multi Regression Analysis Technique. The findings of the research showed that school principals shown mid-level transformational and transactional leadership behaviours, and they were more likely to perform transformational leadership style. Teachers were victims of bullying at low level. As long as primary school principals have more transformational and transactional leadership behaviours, teachers’ mobbing experiences decrease. Idealized influence (behavior and inspirational motivation that sub-dimensions of transformational leadership style and contingent reward that subdimension of transactional leadership style are significant predictor of mobbing. Mobbing that primary school teachers experience can be explained by school principals’ leadership styles

  10. A Rese arch o n Determinig The Effects of Entrepreneurship Tendencies o f The Students Gettıng The Entrepreneurship Education: Sam ple of Honaz Vocational School a t Pamukkale University

    Directory of Open Access Journals (Sweden)

    Ali Soylu

    2015-09-01

    Full Text Available The concept of entrepreneurship both brings solution to the socio-economic problems of many developing countries such as unemployment, job creation, increasing the production and contributes to the national vision onan international platform. One of the most important institutions that contribute to the national vision is the universities. In this context, seeing and guiding the university students as potential entrepreneurs is of particular importance within the framework of national vision and problems.The main aim of the study is to reveal the effects of the entrepreneurship courses on the entrepreneurship orientation of the students. For this purpose, Pamukkale University conducted a field study with students taking entrepreneurship courses in Honaz Vocational School. First of all, concepts on entrepreneurship and entrepreneurial personality are described in the study. Then, statistical analysis was conducted in the context of entrepreneurial personality traits byexamining the aspects of tolerance to uncertainty, risk-taking, innovation, creativity, independence, need for achievement, relationship with people, internal locus of control among the students. Consequently, the level of impact of the entrepreneurship courses on the entrepreneurship orientation of the students was determined

  11. Environmental analysis of alcohol ethoxylates and nonylphenol ethoxylate metabolites by ultra-performance liquid chromatography-tandem mass spectrometry.

    Science.gov (United States)

    Lara-Martín, Pablo A; González-Mazo, Eduardo; Brownawell, Bruce J

    2012-03-01

    Surfactants and their metabolites can be found in aquatic environments at relatively high concentrations compared with other micropollutants due in part to the exceptionally large volumes produced every year. We have focused our attention here on the most widely used nonionic surfactants, alcohol ethoxylates (AEOs), and on nonylphenol ethoxylate (NPEO) degradation products (short-chain nonylphenol ethoxylates, NP1-3EO, nonylphenol, NP, and nonylphenol ethoxycarboxylates, NP1-2EC), which are endocrine-disrupting compounds. Our main objective in this work was to develop a methodology aimed at the extraction, isolation, and improved analysis of these analytes in environmental samples at trace levels. Extraction recoveries of target compounds were determined for sediment samples after ultrasonic extraction and purification using HLB or C18 solid-phase extraction minicolumns. Recovery percentages were usually between 61 and 102% but were lower for longer AEO ethoxymers. Identification and quantification of target compounds was carried out using a novel ultra-performance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS-MS) approach, a combination that provides higher sensitivity and faster analysis than prior methods using conventional high-performance liquid chromatography-mass spectrometry. Limits of detection were usually below 0.5 ng/g, being higher for monoethoxylate species (>5 ng/g) because of poor ionization. The method was used for analyzing surface sediment samples collected at Jamaica Bay (NY) in 2008. The highest values (28,500 ng/g for NP, 4,200 ng/g for NP1-3EO, 22,400 ng/g for NP1-2EC, and 1,500 ng/g for AEOs) were found in a sampling station from a restricted water circulation area that is heavily impacted by wastewater discharges. PMID:22002557

  12. Accurate ab initio structural parameters of the diatomic and triatomic van der Waals molecules (11)BNg (X(2)Π, A(2)Σ(+)) and (11)BNg2 (X̃(2)B1), Ng = (4)He, (20)Ne, (40)Ar, (84)Kr, and (132)Xe.

    Science.gov (United States)

    Magoulas, Ilias; Papakondylis, Aristotle; Mavridis, Aristides

    2014-06-01

    The weakly interacting BNg and BNg2 molecular systems, Ng = He, Ne, Ar, Kr, and Xe, have been thoroughly studied through coupled-cluster RCCSD(T) calculations and large correlation consistent basis sets. For the BNg diatomics, the states examined are the X(2)Π and A(2)Σ(+), and the X̃(2)B1 state for the C2v BNg2 triatomics. A series of corrections render our final results reliable, judging as well from the (limited) experimental numbers available. Both BHe and BHe2 are marginally unbound, whereas the attractive interactions of the BNg X(2)Π states, where Ng = Ne, Ar, Kr, and Xe, are D0 = 19.8, 98.2, 141.9, and 209.1 cm(-1), respectively. For the BRn (Rn = radon) species, an estimated value of interaction energy D0 ≈ 280 cm(-1) is obtained by a D0 versus static polarizability (α) extrapolation. Corresponding atomization energies of the BNg2 (X̃(2)B1) molecules are AE0 = 52.0 (BNe2), 263.4 (BAr2), 384.6 (BKr2), and 576.9 (BXe2) cm(-1). PMID:24806885

  13. Determination of endogenous levels of GHB in human hair. Are there possibilities for the identification of GHB administration through hair analysis in cases of drug-facilitated sexual assault?

    Science.gov (United States)

    Goullé, Jean Pierre; Chèze, Marjorie; Pépin, Gilbert

    2003-01-01

    We have developed a GC-MS-MS assay for GHB in human hair. Five milligrams of washed hair were hydrolyzed by 1M or 0.01M NaOH before a liquid-liquid extraction with ethyl acetate under acidic conditions. GHB-d(6) was used as the internal standard. TMS derivatives were formed before injection. TBDMS derivatives were used in cases of strong chromatographic interferences or in a confirmatory procedure. Analysis of basal levels of GHB in 61 drug-free donors gave the following results: the mean measured concentration for blond hair was 0.60 ng/mg (n = 12), SD = 0.19 ng/mg, and extreme figures were in the range 0.35-0.95 ng/mg. For brown hair, the mean measured concentration was 0.90 ng/mg (n = 30), SD = 0.42 ng/mg, and extreme figures 0.41-1.86 ng/mg. For black hair, the mean measured concentration was 0.90 ng/mg (n = 19), SD = 0.37 ng/mg, and extreme figures 0.32-1.54 ng/mg, showing no significant differences depending on hair color. Analysis of basal levels of GHB of 12 or more specimens in segmented hair showed a mean concentration of 1.22 ng/mg (0.31-8.4 ng/mg) and a relative standard deviation for each individual ranging from 6.75% to 37.98%. GHB was administered to a healthy 53-year-old white male (light brown hair) at oral dosages of 30, 45, and 60 mg/kg. Beard hair was collected just before administration and 24 h after (and each day for one week for the last dose), and a 7.5-cm scalp hair lock was collected 7 days after the last dose. A rise in GHB concentration was observed in beard hair for the 45 and 60 mg/kg dosages with a maximum at 24 h, whereas no change was observed for the 30 mg/kg dosage. Scalp hair was segmented into 3-mm long segments. The three proximal last segments showed significantly (0.0005 rape under the influence of GHB was documented through hair analysis (black hair) and positive analysis of the glass she used. Sampled 7 days after the sexual assault, the three last 3-mm long proximal segments tested for GHB exhibited concentrations of 3

  14. Microemulsion electrokinetic chromatography for analysis of phthalates in soft drinks.

    Science.gov (United States)

    Hsieh, Sung-Yu; Wang, Chun-Chi; Wu, Shou-Mei

    2013-12-15

    Microemulsion electrokinetic chromatography (MEEKC) is proposed for analysis of di-n-butyl phthalate (DBP) and di-(2-ethylhexyl) phthalate (DEHP) in soft drinks. However, the instability of microemulsion is a critical issue. In this research, a novel material, Pluronic® F-127, which has the properties of polymer and surfactant, was added for stabilizing the microemulsion in the MEEKC system. Our data demonstrate that the presence of Pluronic® F-127 (0.05-0.30%) also helps enhance resolution of highly hydrophobic compounds, DBP and DEHP. The electrokinetic injection of sodium dodecyl sulphate (SDS) including sample (-10 kV, 20 s) was introduced in this MEEKC system and this yielded about 25-fold sensitivity enhancement compared with hydrodynamic injection (1 psi, 10 s). During method validation, calibration curves were linear (r≥0.99), within a range of 75-500 ng/mL for DBP and 150-1000 ng/mL for DEHP. As the precision and accuracy assays, absolute values of relative standard deviation (RSD) and relative error (RE) in intraday (n=3) and interday (n=5) observations were less than 4.93%. This method was further applied for analyzing six commercial soft drinks and one was found containing 453.67 ng/mL of DEHP. This method is considered feasible for serving as a tool for analysis of highly hydrophobic molecules. PMID:23993511

  15. Bottled water: analysis of mycotoxins by LC-MS/MS.

    Science.gov (United States)

    Mata, A T; Ferreira, J P; Oliveira, B R; Batoréu, M C; Barreto Crespo, M T; Pereira, V J; Bronze, M R

    2015-06-01

    The presence of mycotoxins in food samples has been widely studied as well as its impact in human health, however, information about its distribution in the environment is scarce. An analytical method comprising a solid phase extraction procedure followed by liquid chromatography tandem mass spectrometry analysis was implemented and validated for the trace analysis of mycotoxins in drinking bottled waters. Limits of quantification achieved for the method were between 0.2ngL(-1) for aflatoxins and ochratoxin, and 2.0ngL(-1) for fumonisins and neosolaniol. The method was applied to real samples. Aflatoxin B2 was the most frequently detected mycotoxin in water samples, with a maximum concentration of 0.48±0.05ngL(-1) followed by aflatoxin B1, aflatoxin G1 and ochratoxin A. The genera Cladosporium, Fusarium and Penicillium were the fungi more frequently detected. These results show that the consumption of these waters does not represent a toxicological risk for an adult. PMID:25624256

  16. Draped heterogeneity, forced uniformity: when agro-environmental policies drive family development: The U Minh Thượng forest reserve, (Mekong delta, Vietnam Hétérogénéité cachée, uniformité forcée : quand les politiques agro-environnementales guident le développement familial – Cas de la réserve forestière de U Minh Thượng (delta du Mékong, Vietnam Heterogeneidad oculta, uniformidad forzada: cuando las políticas agro-ambientales impulsan el desarrollo familiar. La reserva del bosque de U Minh Thượng (delta del Mekong, Vietnam

    Directory of Open Access Journals (Sweden)

    Mehdi Saqalli

    2011-04-01

    Full Text Available The U Minh Thượng natural reserve was created during the 1990s with a surrounding buffer zone where 3,500 households have been settled between 1992 and 1995, each household on a lot of 4 hectares. From a social point of view, the settlers were selected as 'poor people' or war veterans. A social discrimination was slowly built in favour of the latter. Despite an apparent homogeneity, the environment shows slight variations which has huge effects on the potential of the lots. From 1992 to 1998, the provincial government applied uniformly different management policies, despite the fact that this repeated "new deal" of the variability among households and lots, produced various outcomes from total destruction to a real support to households. Finally, the support of international environmental non-governmental organizations enhanced a pro-nature hard-line that has affected farmers' livelihoods. This 'real world' social experiment enhances the necessity to at least understand the needs and constraints at a lower scale, especially for such a vast area.La réserve naturelle de U Minh Thượng a été créée dans les années 1990, ceinturée par une zone tampon où ont été installés 3 500 ménages entre 1992 et 1995. Chaque ménage a reçu un lot de 4 hectares. Sur le plan social, les colons ont été sélectionnés parmi les « pauvres » ou les anciens combattants. Une différenciation sociale s’est progressivement instaurée en faveur de ces derniers. Malgré une homogénéité apparente, le milieu physique montre de légères variations, qui ont des répercussions considérables sur le potentiel des lots. De 1992 à 1998, le gouvernement provincial a appliqué différentes politiques d’aménagement de façon uniforme, bien que cette « nouvelle donne » répétée de la variabilité entre les ménages et les terrains ait des effets mitigés, pouvant aller d’un échec total à un réel soutien aux familles. Enfin, le soutien d

  17. Analysis of multiple anticoagulant rodenticides in animal blood and liver tissue using principles of QuEChERS method.

    Science.gov (United States)

    Vudathala, Daljit; Cummings, Margaret; Murphy, Lisa

    2010-06-01

    A quick and easy method for the analysis of anticoagulant rodenticides in blood or tissue using principles of dispersive solid-phase extraction (dSPE), commonly known as QuEChERS (short for quick, easy, cheap, effective, rugged, and safe), was developed. Briefly, a combination of magnesium sulfate, PSA, florisil, and basic alumina was used to cleanup blood samples. Further, to cleanup liver tissue samples, C(18) sorbent was included along with the previously mentioned. The samples were analyzed using high-performance liquid chromatography equipped with a reversed-phase C(18) column (150 x 4.6 mm, 5-microm particle size) and a UV and fluorescence detector. The mobile phase consisted of 0.03 M tetrabutylammonium hydroxide (TBA) adjusted to pH 7/methanol (1:1, v/v) as solvent A and methanol as solvent B in a gradient run. The method detection limit was as low as 10 ng/mL for brodifacoum and difenacoum in blood and 10 ng/g in liver; 50 ng/mL for bromadiolone, difethialone, and chlorphacinone in blood and similarly 50 ng/g in liver; and 100 ng/mL for coumafuryl, pindone, warfarin, and diphacinone in blood and 100 ng/g in liver samples. A number of clinical samples of both blood and liver were analyzed; the comparison of this modified QuEChERS and traditional solid-phase extraction data was found to be in close agreement. This method resulted in drastic reduction in processing time and solvent cost both in terms of consumption and disposal, thus making it an attractive alternative to the traditional solid-phase extraction. PMID:20529461

  18. Thе іmpаct of fіnаncіаl rеportіng of Smаll аnd Mеdіum Sіzеd Entеrprіsеs іn Economіc Dеvеlopmеnt of Bаlkаn Countrіеs

    Directory of Open Access Journals (Sweden)

    Artor Nuhiu

    2012-04-01

    Full Text Available Thе іmportаncе of іntеrnаl аnd еxtеrnаl аccountіng аs а sourcе of іnformаtіon for ownеrs аnd mаnаgеrs of smаll еntеrprіsеs аnd thеіr dіffеrеnt stаkеholdеrs іs stеаdіly growіng. It іs of crucіаl іmportаncе thаt thе аccountіng systеms аpplіеd by smаll еntеrprіsеs mееt thеіr аctuаl nееds, provіdіng nеcеssаry іnformаtіon yеt аvoіdіng unjustіfіеd аdmіnіstrаtіvе burdеn. It іs rеcognіsеd thаt аpproprіаtе аccountіng іnformаtіon іs іmportаnt for а succеssful mаnаgеmеnt of а busіnеss whеthеr іt іs lаrgе or smаll.At EU lеvеl, аccountіng lеgіslаtіon іs іn plаcе for lіstеd compаnіеs, і.е. thе Intеrnаtіonаl Accountіng Stаndаrds/Intеrnаtіonаl Fіnаncіаl Rеportіng Stаndаrds аnd for non-lіstеd lіmіtеd lіаbіlіty compаnіеs, thе Fourth аnd thе Sеvеnth Compаny Lаw Dіrеctіvеs і.е. thе Accountіng Dіrеctіvеs. Howеvеr, аt EU lеvеl thеrе іs no аccountіng lеgіslаtіon аpplіcаblе to thosе еntеrprіsеs whіch аrе not lіstеd or аrе not lіmіtеd lіаbіlіty compаnіеs; іn most cаsеs wе would bе rеfеrrіng to smаll еntеrprіsеs.Bеcаusе of thе іmportаncе of аpproprіаtе аccountіng іnformаtіon for ownеrs аnd mаnаgеrs of smаll еntеrprіsеs аnd thеіr dіffеrеnt stаkеholdеrs, іt іs consіdеrеd іmportаnt to аnаlysе thе vаrіous аccountіng systеms аpplіеd іn Mеmbеr Stаtеs іn thе cаsе of non-rеgulаtіon аt EU lеvеl.

  19. Selenium determination in cereal plants and cultivation soils by radiochemical neutron activation analysis

    International Nuclear Information System (INIS)

    Selenium (Se) is an essential micronutrient for human health but it is deficient in at least 1 billion people around the globe. Cereals are by far the most significant agricultural crops, not only on a gross tonnage basis, but also by what they represent in terms of energy supply and dietary intake for human nutrition worldwide. Portugal is no exception to such pattern. The Portuguese situation is difficult to assess though, due to scarce information and lack of consistent studies on the subject. In these terms, the Se status of major cereals and their cultivation soils are dealt with herein. Two species of wheat-bread and durum wheat-were sown at the end of November 2009, and then sampled in different growth stages. Rye was collected during harvest season, and cultivation soils were analyzed as well. Se results were within the range of: 100-225 ng g-1 for soils; 3-55 ng g-1 for durum wheat; 6-80 ng g-1 for bread wheat; and 4-30 ng g-1 for rye. Accuracy of the RNAA procedure was proved by analysis of reference materials NIST-SRM 1515 and NIS-SRM 8433. (author)

  20. Chiral analysis of methorphan in opiate-overdose related deaths by using capillary electrophoresis.

    Science.gov (United States)

    Bertaso, Anna; Musile, Giacomo; Gottardo, Rossella; Seri, Catia; Tagliaro, Franco

    2015-09-01

    An enantioselective CE-based determination of methorphan and its main metabolites in blood is described. Enantiomeric separations were carried out in 50cm×50μm (ID) uncoated fused silica capillaries, using a background electrolyte composed of 150mM sodium phosphate pH 4.4 added with 5mM 2-(hydroxypropyl)-β-cyclodextrin and methanol 20% (v/v), at a constant voltage of 25kV. Sample injections were performed under field amplified sample stacking conditions. Detection was by recording UV absorbance at the wavelength of 200nm. Linearity of response was assessed within a concentration range from 25 to 500ng/mL for dextrometorhan, levomethorphan and their main metabolites (namely dextrorphan and levorphanol, respectively). Folcodine was used as internal standard. Under these conditions, the limit of quantification resulted 25ng/mL for each one of the analytes. The intra-day and inter-day precision, in terms of coefficient of variation (CV) were below 3.7% and 14.9 % for migration times and peak areas, respectively. The present method was successfully applied to the analysis of post-mortem blood samples from ten subjects died for heroin overdoses. Among the samples "positive" for methorphan (n=4), the d-enantiomer was found in concentrations ranging from 214 to 1282ng/mL. The concentration of its main metabolite dextrorphan in the same samples ranged from 49 to 389ng/mL. PMID:26226106

  1. Estacionalidad de egresos por neumonía en el Servicio de Pediatría del Hospital "Maximiliano Peralta" y su asociación con la migración indígena Ngöbe Buglé

    OpenAIRE

    María de la Paz Barboza- Argüello; Ana Morice Trejos; María Carranza- Maxera; Lissette Navas-Alvarado

    2005-01-01

    Objetivo: El presente estudio pretende determinar la magnitud del incremento en los casos de neumonía a finales de 2002, y la posible asociación de éste con la migración estacional de indígenas Ngöbe Buglé, a la zona de Los Santos, durante la época de cosecha de café. Metodología: Se analizaron los egresos por neumonía (Clasificación internacional de las CIE10= J18) del Servicio de Pediatría, del Hospital Max Peralta (HMP) durante 1997-2003. Se revisaron los expedientes de los egresos del Ser...

  2. Methodology of simultaneous analysis of Uranium and Thorium by nuclear and atomic techniques. Application to the Uranium and Thorium dosing in mineralogic samples

    International Nuclear Information System (INIS)

    This work concerns essentially the potential applications of 100 kW nuclear reactor of Strasbourg Nuclear Research Centre to neutron activation analysis of Uranium and Thorium. The Uranium dosing has been made using: 239-U, 239-Np, fission products or delayed neutrons. Thorium has been showed up by means of 233-Th or 233-Pa. The 239-U and 233-Th detection leads to a rapid and non-destructive analysis of Uranium and Thorium. The maximum sensitivity is of 78 ng for Uranium and of 160 ng for Thorium. The Uranium and Thorium dosing based on 239-Np and 233-Pa detection needs chemical selective separations for each of these radionuclides. The liquid-liquid extraction has permitted to elaborate rapid and quantitative separation methods. The sensitivities of the analysis after extraction reach 30 ng for Uranium and 50 ng for Thorium. The fission products separation study has allowed to elaborate the La, Ce and Nd extractions and its application to the Uranium dosing gives satisfying results. A rapid dosing method with a sensitivity of 0.35 microgramme has been elaborated with the help of delayed neutrons measurement. These different methods have been applied to the Uranium and Thorium dosing in samples coming from Oklo mine in Gabon. The analyses of these samples by atomic absorption spectroscopy and by the proton induced X-ray emission (PIXE) method confirm that the neutron activation analysis methods are reliable. 37 figs., 14 tabs., 50 refs

  3. IPO. Mäng suure orkestriga / Tiiu Pedaja, Gerd Laub

    Index Scriptorium Estoniae

    Pedaja, Tiiu

    2006-01-01

    Aktsiate avaliku pakkumise ehk IPO korraldamisest. Lisad: Millal on õige aeg?; Milline ettevõte sobib avalikuks pakkumiseks?; Milline avalik pakkumine on edukas?; Kuidas IPO-l aktsiaid jaotatakse? Skeemid: Aktsia hinna kujunemine avaliku pakkumise käigus; Järelturu aktsiahinda mõjutavad tegurid

  4. LEIOMYOMA, A MAJOR CAUSE OF ABNORMAL UTERINE BLEEDI NG

    Directory of Open Access Journals (Sweden)

    Sreeja Rani

    2013-04-01

    Full Text Available ABSTRACT: INTRODUCTION: Abnormal uterine bleeding is a common complai nt for which many patients in the perimenopausal age group un dergo hysterectomy. The objective of this study is to analyze the causes of AU B in subjects who had hysterectomy. MATERIAL AND METHODS: The pathological specimens from 100 patients who had hysterectomy for AUB between January 2012 and January 2013 we re studied based on the gross and microscopic appearance. RESULTS: Leiomyoma alone was the most common cause of AUB (54% in patients who had hysterectomy. Leiomy oma was associated with adenomyosis in 20% cases and adenomyosis alone was seen in 10%. Endometrial cancer was found in 2% of patients. CONCLUSION: Leiomyoma is the most common indication for hysterectomy in women with AUB between 40 and 50 years. It may occur alone or may be associated with conditions like adenomyosis or endometria l polyps

  5. Uus rahvuslik ringmäng : SAPARD / Jaanus Marrandi

    Index Scriptorium Estoniae

    Marrandi, Jaanus, 1963-

    2001-01-01

    Tõenäoliselt ei jõua SAPARD-i raha ka 2001. aastal põllumeheni. Autor: Keskerakond. Parlamendisaadik. Ilmunud ka: Sakala, 25. apr. 2001, lk. 2; Nädaline, 24. apr. 2001, lk. 4; Järva Teataja, 4. mai 2001, lk. 2; Pärnu Postimees, 4. mai 2001, lk. 2; Meie Maa, 3. mai 2001, lk. 6; Võrumaa Teataja, 15. mai 2001, lk. 2; Hiiu Leht, 29. mai 2001, lk. 2

  6. Language Learnıng Strategy Preferences of Turkish Students

    OpenAIRE

    Selma DENEME

    2008-01-01

    A learning style is a preferred way of acquiring knowledge and processing information. Learning styles may differ depending on gender, age, or culture. Niles (1995) in his study “Cultural Differences in Learning Motivation and Learning Strategies” studied the cultural impact on learning strategies and found considerable differences between culturally different students. Providing evidence for the relationship between culture and learning strategy preference and use, this study aims at examini...

  7. Sügisene moevaatemäng / Britt Rosen

    Index Scriptorium Estoniae

    Rosen, Britt, 1968-

    2008-01-01

    FIBITi moepäevad Viru keskuses, korraldaja Anu Kikas. Arne Niidu, Anu Samarüütel-Longi, Tanel Veenre kollektsioonidest. Kalle Aasamäe, Lilli Jahilo, Aet Alevi, Anu Samarüütel-Longi ja Tanel Veenre kommentaarid

  8. Improviseeritud lugu ja zhanriga mäng / Donald Tomberg

    Index Scriptorium Estoniae

    Tomberg, Donald

    2003-01-01

    Eesti komöödiafilm "Vanad ja kobedad saavad jalad alla" : režissöör Rando Pettai : operaator Mait Mäekivi : näitlejad Henrik Normann, Madis Milling, Robert Gutman jt. : muusika - ansambel Genialistid : Ruut Pictures 2003

  9. Robotteknologi i en undervisningssammenhæng

    DEFF Research Database (Denmark)

    Majgaard, Gunver

    læringskoncepter. Artiklen kredser i feltet kropslig forbundet intelligens, menneske-robot interaktion og læring. Mead bruges som løftestang til at forstå de sociale aspekter i interaktionen mellem menneske og robot. Batesons taksonomi over læringsniveauer bruges til at diskutere lærings og interaktionspotentiale...

  10. Nukumäng edasijõudnutele / Heidi Aadma

    Index Scriptorium Estoniae

    Aadma, Heidi, 1976-

    2008-01-01

    Eesti Nuku- ja Noorsooteatris etendunud Tšehhis elava tšerkessi Jevgeni Ibragimovi nukulavastustest "Väga lihtne lugu" (autor Maria Lado), "Nukumängu ABC", "Mängurid" (autor Nikolai Gogol), "Oskar ja Roosa Mamma" (autor E.-E. Schmitt)

  11. Checkpoint NG VPN 1firewall 1 advanced configuration and troubleshooting

    CERN Document Server

    Syngress

    2003-01-01

    Check Point Software Technologies is the worldwide leader in securing the Internet. The company''s Secure Virtual Network (SVN) architecture provides the infrastructure that enables secure and reliable Internet communications. Check Point recently announced a ground-breaking user interface that meets the industry''s next generation Internet security requirements, including simplified security management for increasingly complex environments. Built upon Check Point''s Secure Virtual Network (SVN) architecture, the Next Generation User Interface revolutionizes the way security administrators define and manage enterprise security by further integrating management functions into a security dashboard and creating a visual picture of security operations. The Next Generation User Interface delivers unparalleled ease-of-use, improved security and true end-to-end security management. Check Point''s revenues have more than doubled in each of the last two years, while capturing over 50% of the VPN market and over 40% of...

  12. ”Sammenhæng mellem kursus og praksis”

    DEFF Research Database (Denmark)

    Hansen, Bodil Winther; Bundgaard, Annette; Nysteen, Eva

    2003-01-01

    med 3 teorier, der tager udgangspunkt i praksis og lægger vægt på samspillet mellem individ og omgivelser: • Virksomhedsteori repræsenteret af Vygotsky, Leontjev og Engeström • Teorier om praksislæringved Schön og desuden er inddraget hans arbejde med organisatorisk læring foretaget i samarbejde med...

  13. DOPİNG VE SPORCULARDA KULLANIMI / DOPING AND USAGE

    OpenAIRE

    Çınar, Vedat; Öztürk, M. Ertuğrul; Şebin, Kenan; Yazıcı, Gökhan

    2010-01-01

    Performans fizik, kondisyon, beceri, koordinasyon, kas gücü, dayanıklılık ve dengeli beslenme gibibirçok faktörle artınıabilir. Sporcuların perfonnanstarını arttıona arzusu kuvvetli bir arzudur ve sportifbaşarının gerek ekonomik gerek sosyal getirileri, bu başarılı olma arLUsunu ahlaki kuralları olduğukadar sağlık kurallarını da bozmasına neden olmaktadır.Yüzyıllar boyunca insanoğlu iş verimin arttırmak, başanya ulaşmak zaferler kazanmak veya sportifperfonnansı artt...

  14. No Kiss1ng by leptin during puberty?

    OpenAIRE

    Ahima, Rexford S.

    2010-01-01

    Leptin exerts a permissive action on puberty by stimulating release of gonadotropin-releasing hormone (GnRH) in the hypothalamus. However, GnRH neurons lack leptin receptor (LepR), indicating that leptin must indirectly regulate these neurons. The Kiss1 gene produces kisspeptins that stimulate GnRH secretion. Because Kiss1 neurons express LepR and inactivation of Kiss1 causes hypogonadotropic hypogonadism, Donato et al., in this issue of the JCI, assessed whether deletion of LepR from Kiss1 n...

  15. Kollisioner igång till hösten

    CERN Multimedia

    Hellman, STen

    2009-01-01

    "Der började så bra. Den 10 september var utannonserad som dagen då CERNs nya accelerator LHC för första gången skulle ta emot protoner och losta dem ett helt varv runt den 27 km långa acceleratorringen" (3 pages)

  16. 58. "Berlinale" lõppmäng / Jaak Lõhmus

    Index Scriptorium Estoniae

    Lõhmus, Jaak

    2008-01-01

    Berliini 58. filmifestivali auhinnasaajatest : Kuldkaru võitis Brasiilia produtsendi ja režissööri Jose Padilha mängufilm "Eliitrühm", Hõbekaru USA režissööri Errol Morrise dokfilm "Tavaprotseduur". Parima režissööri Hõbekaru läks Paul Thomas Andersonile mängufilmi "Veri hakkab voolama" eest. Parima näitleja auhinna said Sally Hawkins ja Reza Naji, Alfred Baueri auhinna sai Fernando Eimbcke "Tahoe järv"

  17. Tillgång till skolbibliotek i glesbygd

    OpenAIRE

    Gällman, Johanna

    2013-01-01

    This is a two years master’s thesis in Archive, Library and Museum studies, which purpose is to find out what ideas there are about school libraries and to get an idea of how informants interpret the new Education Act, which requires all students to have access to school libraries. Another intention of this paper is to highlight the potential rural-related attitudes of the responents. The study includes the sparsely populated municipality of Krokom and is based on interviews with librarians, ...

  18. Nicanor Abelardo: Tagapagbunsod ng Modernismo sa Sining Musika sa Pilipinas

    Directory of Open Access Journals (Sweden)

    Ramon P. Santos

    2001-12-01

    Full Text Available The twentieth century has borne witness to some of the great changes in the musical art of Europe and the West. The historical events of the past century in economics and education, the speedy spread of intellectualization and the achievements in science all contributed to the creation of new perspectives in culture and the arts. These perspectives, which were reactions to the aesthetics of the time and to the accepted styles of expression, led to a major movement called "modernism." The various directions and views which formed the major characteristics of modernism in music came together under the aegis of freedom, freedom from the old ways. This freedom was seen as a departure from emotion and sentimentalism as well as emancipation from the long imprisonment in the system of tonal harmony, which in turn suppressed emotional breadth and human thought. While studying in America, Nicanor Abelardo embraced contemporary philosophy and techniques. This brought about a modern quality to his works during his last years. This article is an initial study of this rapid and dramatic metamorphosis in the art of NIcanor Abelardo.

  19. Biennaal ehk mäng neljale keelele / Johannes Saar

    Index Scriptorium Estoniae

    Saar, Johannes, 1965-

    2001-01-01

    49. Veneetsia biennaali korraldusest, probleemidest, kontseptsioonist. Loetletud preemiasaajad - peapreemiad, rahvuspaviljoni Kuldlõvi, Kuldlõvi tänapäevakunsti meistritele ja noore kunstniku preemiad

  20. "Klaaspärlimäng" kolis Tartusse

    Index Scriptorium Estoniae

    2008-01-01

    Anne Veskilt ilmuvast singlist "Kallis Eestimaa". Tartu laululava muruplatsil toimuvast sõltumatu muusika festivalist Plink Plonk. Viljandi Folgist. Muusikavõrgustik Last.fm viis ilma plaadifirmata bändidele sisse autoritasude süsteemi, mille alusel muusikud saavad raha oma lugude pealt, mis Last.fm-i muusikakogusse lisatakse ja seal kuulatakse

  1. Test Automation Selenium WebDriver using TestNG

    OpenAIRE

    Bindal, Purnima; Gupta, Sonika

    2014-01-01

    Test automation tools (softwares) are used to control the execution of tests (Application Under Test) and then comparing actual outcomes to the predicted outcomes. Examples of Test Automation tools are HP Quick Test Professional, Selenium, Test Complete, SOAP UI etc. Selenium is a suite of tools for cross-platform automated testing of web applications. It runs on many browsers and operating systems and can be controlled by many programming languages and testing frameworks. Selenium W...

  2. Kriisikoldeid kustutab (teatri)mäng / Rait Avestik

    Index Scriptorium Estoniae

    Avestik, Rait, 1974-

    2010-01-01

    Itaallaste Nino d’Introna ja Giacomo Ravicchio teksti põhjal valminud seiklusloost „Robinson & Crusoe”, tõlkinud Kirke Org, lavastanud Aare Toikka. Esietendus 27. nov. 2009 VAT-teatris. Mängivad Meelis Põdersoo ja Ago Soots

  3. ng närvide peale / Argo Ideon

    Index Scriptorium Estoniae

    Ideon, Argo, 1966-

    2006-01-01

    Autor on seisukohal, et Keskerakonna ja nelja erakonna viimased kohtumised-avaldused on võistlus avalikkuse ees. Keskerakonna juhatuse ja parlamendiliikmete kohtumisest Toomas Hendrik Ilvese ja Ene Ergmaga. Peeter Kreitzberg vastab küsimustele, kuidas temast sai 2001. aastal presidendikandidaat, milline oli tegelik põhjus, miks Edgar Savisaar ise ei kandideerinud ja kas P. Kreitzbergil oli tookord šanss presidendiks saada

  4. Vastutustundetu mäng eelarvega / Andrus Villem

    Index Scriptorium Estoniae

    Villem, Andrus

    1998-01-01

    Eelarve võetakse arvatavasti vastu alles jaanuaris. Keskerakond on kahepalgeline. Ilmunud ka: Nädaline, 17. nov. 1998, lk. 2; Järva Teataja, 24. nov. 1998, lk. 2; Severnoje Poberezhje, 12. nov. 1998, lk. 5

  5. Musta notsu mäng maailma finantsturgudel / Sven Meimer

    Index Scriptorium Estoniae

    Meimer, Sven

    2007-01-01

    Maailmas on tekkinud äri, kus laenu jagatakse sisuliselt laenuvõimetutele isikutele (USA-s tuntud nime all sub-prime) ning tekitatakse keerulisi laenusaajaid ja finantsettevõtteid raskustesse viivaid garantiiskeeme

  6. Arkitekturens kroppslighet. Staden som terräng

    OpenAIRE

    Nilsson, Emma

    2010-01-01

    Beginning with the notion that spatial and material qualities of architecture set limits and create conditions for how we make use of it, the overall aim and ambition of this thesis is to develop this notion and to contribute to a deeper understanding of the relationships that are produced between the materiality of architecture and a bodily appropriation of it. To investigate this the thesis establishes two concepts: corporality and terrain. The concept of corporality recognizes ...

  7. Analysis of testosterone in human urine using molecularly imprinted solid-phase extraction and corona discharge ion mobility spectrometry.

    Science.gov (United States)

    Mirmahdieh, Shiva; Mardihallaj, Azam; Hashemian, Zahra; Razavizadeh, Jalal; Ghaziaskar, Hassan; Khayamian, Taghi

    2011-01-01

    Analysis of testosterone was accomplished using corona discharge ion mobility spectrometry. Molecular imprinted polymer was used for the extraction and pre-concentration of testosterone. Analytical parameters including precision, dynamic range and detection limit were obtained. The linear dynamic range was from 10 to 250 ng/mL and the limit of detection was 0.9 ng/mL. The proposed method was used for analysis of testosterone in urine samples. A urine sample from a 3-year-old girl was used as the blank. The RSD was below 10%. The obtained results from the method were also compared with the standard method for analysis of testosterone using SPE-HPLC analysis. The results demonstrate the accuracy of the method. PMID:21171183

  8. A Cross Sectional Analysis of Gonococcal and Chlamydial Infections among Men-Who-Have-Sex-with-Men in Cape Town, South Africa.

    Directory of Open Access Journals (Sweden)

    Kevin Rebe

    Full Text Available Men-who-have-sex-with-men (MSM are at high risk of HIV and sexually transmitted infection (STI transmission. Asymptomatic STIs are common in MSM and remain undiagnosed and untreated where syndromic management is advocated. Untreated STIs could be contributing to high HIV rates. This study investigated symptomatic (SSTI and asymptomatic STIs (ASTIs in MSM in Cape Town.MSM, 18 years and above, were enrolled into this study. Participants underwent clinical and microbiological screening for STIs. Urine, oro-pharyngeal and anal swab specimens were collected for STI analysis, and blood for HIV and syphilis screening. A psychosocial and sexual questionnaire was completed. STI specimens were analysed for Neisseria gonorrhoeae (NG and Chlamydia trachomatis (CT infection.200 MSM were recruited with a median age of 32 years (IQR 26-39.5. Their median number of sex partners within the last year was 5 (IQR 2-20. 155/200 (78% reported only male sex partners while 45/200 (23% reported sex with men and women. 77/200 (39% reported transactional sex. At enrolment, 88/200 (44% were HIV positive and 8/112 (7% initially HIV-negative participants seroconverted during the study. Overall, 47/200 (24% screened positive for either NG or CT. There were 32 MSM (16% infected with NG and 7 (3.5% of these men had NG infections at two anatomical sites (39 NG positive results in total. Likewise, there were 23 MSM (12% infected with CT and all these men had infections at only one site. Eight of the 47 men (17% were infected with both NG and CT. ASTI was more common than SSTI irrespective of anatomical site, 38 /200 (19% versus 9/200 (5% respectively (p<0.001. The anus was most commonly affected, followed by the oro-pharynx and then urethra. Asymptomatic infection was associated with transgender identity (OR 4.09 CI 1.60-5.62, ≥5 male sex partners in the last year (OR 2.50 CI 1.16-5.62 and transactional sex (OR 2.33 CI 1.13-4.79 but not with HIV infection.Asymptomatic STI was

  9. Analysis of biomagnification of persistent organic pollutants in the aquatic food web of the Mekong Delta, South Vietnam using stable carbon and nitrogen isotopes.

    Science.gov (United States)

    Ikemoto, Tokutaka; Tu, Nguyen Phuc Cam; Watanabe, Michio X; Okuda, Noboru; Omori, Koji; Tanabe, Shinsuke; Tuyen, Bui Cach; Takeuchi, Ichiro

    2008-05-01

    The present study elucidated the biomagnification profiles of persistent organic pollutants (POPs) through a tropical aquatic food web of Vietnam based on trophic characterization using stable nitrogen analysis. Various biological samples collected from the main stream of the Mekong Delta were provided for the analysis for both POPs, and stable nitrogen and carbon isotope ratios. Of the POPs analyzed, dichlorodiphenyltrichloroethane and its metabolites (DDTs) were the predominant contaminants with concentrations ranging from 0.058 to 12 ng/g wet weight, followed by polychlorinated biphenyls (PCBs) at 0.017-8.9 ng/g, chlordane compounds (CHLs) at 0.0043-0.76 ng/g, tris-4-chlorophenyl methane (TCPMe) at N.D.-0.26 ng/g, hexachlorocyclohexane isomers (HCHs) at N.D.-0.20 ng/g and hexachlorobenzene (HCB) at 0.0021-0.096 ng/g. Significant positive increases of concentrations in DDTs, CHLs, and TCPMe against the stable nitrogen ratio (delta(15)N) were detected, while, concentrations of HCHs and HCB showed no significant increase. The slopes of the regression equations between the log-transformed concentrations of these POPs and delta(15)N were used as indices of biomagnification. The slopes of the POPs for which positive biomagnification was detected ranged from 0.149 to 0.177 on a wet weight basis. The slopes of DDTs and CHLs were less than those reported for a marine food web of the Arctic Ocean, indicating that less biomagnification had occurred in the tropical food web. Of the isomers of CHLs, unlike the studies of the Arctic Ocean, oxychlordane did not undergo significant biomagnification through the food web of the Mekong Delta. This difference is considered to be due to a lack of marine mammals, which might metabolize cis- and trans-chlordane to oxychlordane, in the Mekong Delta ecosystem. The biomagnification profile of TCPMe is reported for the first time in the present study. PMID:18313720

  10. Ang Nagbabagong Anyo ng Sosyolohiya sa Nagbabagong Lipunan at Mundo: Ang Postmodernismo at ang Hinaharap ng Sosyolohiya

    Directory of Open Access Journals (Sweden)

    Gerry M. Lanuza

    2000-12-01

    Full Text Available Social theory today is undergoing a crisis. This crisis is brought about by the postmodern turn in social theory. In this essay postmodernism is defined both as a cultural movement and as the social condition of late capitalism. This paper deals with the restructuring of sociological theory as a result of this postmodern turn. Then, the implications of postmodern theory are discussed in relation to the future of doing a postcolonial sociology in the Philippines.

  11. Yang Pagbul’lók-bul’lok: Varyasyon ng Wikang Mandaya sa Ilang Munisipalidad ng Davao Oriental

    Directory of Open Access Journals (Sweden)

    Dr. Raymund M. Pasion

    2015-05-01

    Full Text Available The study aimed to find out the lexical, phonological, and morphological variations of the Mandaya language in the municipalities of Manay, Caraga, Baganga and Cateel in the province of Davao Oriental. This research was conducted to meet the following objectives: (1 To present the terminologies used in the four municipalities based on the Indigenous Knowledge System and Practices of Mandaya in cultural farming, hunting, fishing and animal husbandry. (2 To identify the variations in the Mandaya language based on lexicon, phonology and morphology. (3 To show the distinct linguistic feature in the Mandaya language that is different from Filipino language based on lexicon, phonology and morphology. Qualitative design, descriptive and indigenous methods were used in gathering and analyzing the data. Purposive combination and snow-ball sampling were utilized in choosing the informants. It was found out in the study that there was a variation of the Mandaya language in the four municipalities. In the lexical aspect, there were three evident categories: the different in form; the slightly similar in form; and the same in form but different in pronunciation. While in the phonological aspect, it also showed three variations; the phoneme /h/; the phoneme /ᵊ/ (swa; and the glide l or /l−l/. On the other hand, in the morphological aspect, the researcher observed two things: the affix and morphological phenomena occurring in the words in different dialects in Mandaya. In general, the occurrence of variations in lexical, phonological and morphological aspects in the Mandaya language was inevitable, and this was caused by the three factors: the linguistics, sociolinguistics and psychological.

  12. Determination of Rh, Pd and Pt in urine samples using a pre-concentration sequential injection analysis system coupled to a quadrupole-inductively coupled plasma-mass spectrometer

    International Nuclear Information System (INIS)

    The proposed flow system was developed in order to minimize the drawbacks related to the PGEs determination by quadrupole-inductively coupled plasma-mass spectrometry (Q-ICP-MS). It was intended not only to lower the limits of detection (LODs) but also to eliminate the interferences originating from some atomic and molecular ions produced in the argon plasma. This was accomplished by means of an on-line sample clean-up/pre-concentration step, using a chelating resin (MetalfixTM ChelamineTM) in which Rh, Pd and Pt were preferably retained when compared with the interfering species. The results obtained by using the developed flow system in the analysis of urine samples are presented. With a sampling rate of 9 samples h-1 (i.e., 27 determinations) and a sample consumption of ca. 10 mL, the developed flow system allowed linear calibration plots up to 100 ng L-1 with detection limits of 1.2 ng L-1 (Rh), 0.4 ng L-1 (Pd) and 0.9 ng L-1 (Pt). Repeatability studies showed good precision (R.S.D.%, n = 5): 3.7% (Rh); 2.6% (Pd) and 2.4% (Pt), for 10 ng L-1; 2.4% (Rh); 1.4% (Pd) and 1.9% (Pt), for 50 ng L-1; and 1.3% (Rh); 0.58% (Pd) and 0.62% (Pt), for 100 ng L-1. By spiking human urine samples, recovery tests were performed, and the values obtained ranged between 89% and 105% (Rh); 90% and 104% (Pd); and 93% and 105% (Pt)

  13. Proteomics Analysis for Finding Serum Markers of Ovarian Cancer

    Directory of Open Access Journals (Sweden)

    Yushan Cheng

    2014-01-01

    Full Text Available A combination of peptide ligand library beads (PLLB and 1D gel liquid chromatography-mass spectrometry/mass spectrometry (1DGel-LC-MS/MS was employed to analyze serum samples from patients with ovarian cancer and from healthy controls. Proteomic analysis identified 1200 serum proteins, among which 57 proteins were upregulated and 10 were downregulated in the sera from cancer patients. Retinol binding protein 4 (RBP4 is highly upregulated in the ovarian cancer serum samples. ELISA was employed to measure plasma concentrations of RBP4 in 80 samples from ovarian cancer patients, healthy individuals, myoma patients, and patients with benign ovarian tumor, respectively. The plasma concentrations of RBP4 ranging from 76.91 to 120.08 ng/mL with the mean value 89.13±1.67 ng/mL in ovarian cancer patients are significantly higher than those in healthy individuals (10.85±2.38 ng/mL. Results were further confirmed with immunohistochemistry, demonstrating that RBP4 expression levels in normal ovarian tissue were lower than those in ovarian cancer tissues. Our results suggested that RBP4 is a potential biomarker for diagnostic of screening ovarian cancer.

  14. Amitriptyline poisoning of a baby: how informative can hair analysis be?

    Science.gov (United States)

    Allibe, Nathalie; Eysseric-Guerin, Hélène; Kintz, Pascal; Bartoli, Mireille; Bost-Bru, Cécile; Grenier, Florian; Scolan, Virginie; Stanke-Labesque, Françoise

    2015-04-01

    We reported a case of a 6-month-old baby girl who was hospitalized in the pediatric emergency for central nervous system disorders then coma. Toxicology analysis showed the presence of amitriptyline (AMI) and its metabolite nortriptyline (NOR) in blood and urine of the baby. Additional investigations suggested a shaken baby syndrome. Given the family context, a judge ordered hair tests for both the child and his parents to document drug exposure. A liquid chromatography tandem mass spectrometric (LC-MS/MS) method was then developed to quantify AMI and NOR in hair. After decontamination and segmentation, 20 mg of hair was incubated overnight at 55 °C in methanol (MeOH). The LC-MS/MS method used an online solid phase extraction and the analysis was performed using two transitions per compound. The LOQ and LOD for the two compounds were estimated at 0.0075 ng/mg and 0.005 ng/mg respectively. All hair segments tested for both parents were negative. For the baby two strands of hair were collected one day after the acute intoxication for the first and 5 weeks later for the second. The first strand was not decontaminated before analysis to avoid losing specimen. The high and relatively homogenous concentrations of AMI (with a range of value from 6.65 to 9.69 ng/mg) and NOR (with a range of value from 7.12 to 8.96 ng/mg) measured suggested that contamination could have occurred. The analysis of the second strand after decontamination allowed to detect AMI and NOR in all hair segments. The obtained values varied between 0.54 and 1.41 ng/mg for AMI and between 1.26 and 4.00 ng/mg for NOR. These results supported the hypothesis of a chronic exposure during several months before hair collection with regular increase. However a single overdose could not be totally excluded. The interpretation of results must take into account the pharmacological and physiological parameters of hair of the children. PMID:25676714

  15. Gas chromatography/ion trap mass spectrometry applied for the analysis of triazine herbicides in environmental waters by an isotope dilution technique

    International Nuclear Information System (INIS)

    A gas chromatography/ion trap mass spectrometry method was developed for the analysis of simazine, atrazine, cyanazine, as well as the degradation products of atrazine, such as deethylatrazine and deisopropylatrazine in environmental water samples. Isotope dilution technique was applied for the quantitative analysis of atrazine in water at low ng/l levels. One liter of water sample spiked with stable isotope internal standard atrazine-d5 was extracted with a C18 solid-phase extraction cartridge. The analysis was performed on an ion trap mass spectrometer operated in MS/MS method. The extraction recoveries were in the range of 83-94% for the triazine herbicides in water at the concentrations of 24, 200, and 1000 ng/l, while poor recoveries were obtained for the degradation products of atrazine. The relative standard deviation (R.S.D.) were within the range of 3.2-16.1%. The detection limits of the method were between 0.75 and 12 ng/l when 1 l of water was analyzed. The method was successfully applied to analyze environmental water samples collected from a reservoir and a river in Hong Kong for atrazine detected at concentrations between 3.4 and 26 ng/l

  16. Analysis of 5-hydroxymethylfurfural in foods by gas chromatography-mass spectrometry.

    Science.gov (United States)

    Teixidó, E; Santos, F J; Puignou, L; Galceran, M T

    2006-11-24

    A new, simple and selective method for the analysis of 5-hydroxymethylfurfural (HMF) in foods by gas chromatography coupled to mass spectrometry (GC-MS) is proposed. Several derivatising procedures based on the formation of an HMF silylated derivative using different reagents were studied. Among the derivatising reagents examined, N,O-bis-trimethylsilyltrifluoroacetamide (BSTFA) provided the best derivatisation yield. Sample clean-up was also optimised, using either liquid-liquid extraction with dichloromethane or solid-phase extraction (SPE) with several commercially available cartridges, and the best results were obtained using ENV+ cartridges. Quality parameters such as day-to-day and run-to-run precision (RSD<10%), linearity (between 25 and 700 ng g(-1)) and detection limit (6 ng g(-1)) were established. This method was successfully applied to the analysis of HMF content in several Spanish food samples from a local market, such as jam, honey, orange juice and bakery products. PMID:17010355

  17. Wastewater micropollutants as tracers of sewage contamination: analysis of combined sewer overflow and stream sediments.

    Science.gov (United States)

    Hajj-Mohamad, M; Aboulfadl, K; Darwano, H; Madoux-Humery, A-S; Guérineau, H; Sauvé, S; Prévost, M; Dorner, S

    2014-01-01

    A sensitive method was developed to measure the sediment concentration of 10 wastewater micropollutants selected as potential sanitary tracers of sewage contamination and include: nonsteroidal anti-inflammatory drugs (acetaminophen - ACE and diclofenac - DIC), an anti-epileptic drug (carbamazepine - CBZ), a β-blocker (atenolol - ATL), a stimulant (caffeine - CAF), a bronchodilator (theophylline - THEO), steroid hormones (progesterone - PRO and medroxyprogesterone - MedP), an artificial sweetener (aspartame - APM) and personal care products (N,N-diethyl-3-methylbenzamide - DEET). Natural sediments (combined sewer overflow and stream sediments) were extracted by ultrasonic-assisted extraction followed by solid-phase extraction. Analyses were performed using ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) using atmospheric pressure chemical ionisation in positive mode (APCI+) with a total analysis time of 4.5 min. Method detection limits were in the range of 0.01 to 15 ng g(-1) dry weight (dw) for the compounds of interest, with recoveries ranging from 75% to 156%. Matrix effects were observed for some compounds, never exceeding |±18%|. All results displayed a good degree of reproducibility and repeatability, with relative standard deviations (RSD) of less than 23% for all compounds. The method was applied to an investigation of stream and combined sewer overflow sediment samples that differed in organic carbon contents and particle size distributions. Acetaminophen, caffeine and theophylline (as confounded with paraxanthine) were ubiquitously detected at 0.13-22 ng g(-1) dw in stream bed sediment samples and 98-427 ng g(-1) dw in combined sewer overflow sediment samples. Atenolol (80.5 ng g(-1) dw) and carbamazepine (54 ng g(-1) dw) were quantified only in combined sewer overflow sediment samples. The highest concentrations were recorded for DEET (14 ng g(-1) dw) and progesterone (11.5 ng g(-1) dw) in stream bed and combined

  18. Comparison of GC-ICP-MS and HPLC-ICP-MS for species-specific isotope dilution analysis of tributyltin in sediment after accelerated solvent extraction

    International Nuclear Information System (INIS)

    This study describes a direct comparison of GC and HPLC hyphenated to ICP-MS determination of tributyltin (TBT) in sediment by species-specific isotope dilution analysis (SS-IDMS). The certified reference sediment PACS-2 (NRC, Canada) and a candidate reference sediment (P-18/HIPA-1) were extracted using an accelerated solvent extraction (ASE) procedure. For comparison of GC and LC methods an older bottle of PACS-2 was used, whilst a fresh bottle was taken for demonstration of the accuracy of the methods. The data obtained show good agreement between both methods for both the PACS-2 sediment (LC-ICP-IDMS 828±87 ng g-1 TBT as Sn, GC-ICP-IDMS 848±39 ng g-1 TBT as Sn) and the P-18/ HIPA-1 sediment (LC-ICP-IDMS 78.0±9.7 ng g-1 TBT as Sn, GC-ICP-IDMS 79.2±3.8 ng g-1 TBT as Sn). The analysis by GC-ICP-IDMS offers a greater signal-to-noise ratio and hence a superior detection limit of 0.03 pg TBT as Sn, in the sediment extracts compared to HPLC-ICP-IDMS (3 pg TBT as Sn). A comparison of the uncertainties associated with both methods indicates superior precision of the GC approach. This is related to the better reproducibility of the peak integration, which affects the isotope ratio measurements used for IDMS. The accuracy of the ASE method combined with HPLC-ICP-IDMS was demonstrated during the international interlaboratory comparison P-18 organised by the Comite Consultatif pour la Quantite de Matiere (CCQM). The results obtained by GC-ICP-IDMS for a newly opened bottle of PACS-2 were 1087±77 ng g-1 Sn for DBT and 876±51 ng g-1 Sn for TBT (expanded uncertainties with a coverage factor of 2), which are in good agreement with the certified values of 1090±150 ng g-1 Sn and 980±130 ng g-1 Sn, respectively. (orig.)

  19. Comparison of GC-ICP-MS and HPLC-ICP-MS for species-specific isotope dilution analysis of tributyltin in sediment after accelerated solvent extraction

    Energy Technology Data Exchange (ETDEWEB)

    Wahlen, Raimund; Wolff-Briche, Celine [LGC Limited, Queens Road, TW11 0LY, Teddington, Middlesex (United Kingdom)

    2003-09-01

    This study describes a direct comparison of GC and HPLC hyphenated to ICP-MS determination of tributyltin (TBT) in sediment by species-specific isotope dilution analysis (SS-IDMS). The certified reference sediment PACS-2 (NRC, Canada) and a candidate reference sediment (P-18/HIPA-1) were extracted using an accelerated solvent extraction (ASE) procedure. For comparison of GC and LC methods an older bottle of PACS-2 was used, whilst a fresh bottle was taken for demonstration of the accuracy of the methods. The data obtained show good agreement between both methods for both the PACS-2 sediment (LC-ICP-IDMS 828{+-}87 ng g{sup -1} TBT as Sn, GC-ICP-IDMS 848{+-}39 ng g{sup -1} TBT as Sn) and the P-18/ HIPA-1 sediment (LC-ICP-IDMS 78.0{+-}9.7 ng g{sup -1} TBT as Sn, GC-ICP-IDMS 79.2{+-}3.8 ng g{sup -1} TBT as Sn). The analysis by GC-ICP-IDMS offers a greater signal-to-noise ratio and hence a superior detection limit of 0.03 pg TBT as Sn, in the sediment extracts compared to HPLC-ICP-IDMS (3 pg TBT as Sn). A comparison of the uncertainties associated with both methods indicates superior precision of the GC approach. This is related to the better reproducibility of the peak integration, which affects the isotope ratio measurements used for IDMS. The accuracy of the ASE method combined with HPLC-ICP-IDMS was demonstrated during the international interlaboratory comparison P-18 organised by the Comite Consultatif pour la Quantite de Matiere (CCQM). The results obtained by GC-ICP-IDMS for a newly opened bottle of PACS-2 were 1087{+-}77 ng g{sup -1} Sn for DBT and 876{+-}51 ng g{sup -1} Sn for TBT (expanded uncertainties with a coverage factor of 2), which are in good agreement with the certified values of 1090{+-}150 ng g{sup -1} Sn and 980{+-}130 ng g{sup -1} Sn, respectively. (orig.)

  20. Comparison of GC-ICP-MS and HPLC-ICP-MS for species-specific isotope dilution analysis of tributyltin in sediment after accelerated solvent extraction.

    Science.gov (United States)

    Wahlen, Raimund; Wolff-Briche, Céline

    2003-09-01

    This study describes a direct comparison of GC and HPLC hyphenated to ICP-MS determination of tributyltin (TBT) in sediment by species-specific isotope dilution analysis (SS-IDMS). The certified reference sediment PACS-2 (NRC, Canada) and a candidate reference sediment (P-18/HIPA-1) were extracted using an accelerated solvent extraction (ASE) procedure. For comparison of GC and LC methods an older bottle of PACS-2 was used, whilst a fresh bottle was taken for demonstration of the accuracy of the methods. The data obtained show good agreement between both methods for both the PACS-2 sediment (LC-ICP-IDMS 828+/-87 ng g(-1) TBT as Sn, GC-ICP-IDMS 848+/-39 ng g(-1) TBT as Sn) and the P-18/ HIPA-1 sediment (LC-ICP-IDMS 78.0+/-9.7 ng g(-1) TBT as Sn, GC-ICP-IDMS 79.2+/-3.8 ng g(-1) TBT as Sn). The analysis by GC-ICP-IDMS offers a greater signal-to-noise ratio and hence a superior detection limit of 0.03 pg TBT as Sn, in the sediment extracts compared to HPLC-ICP-IDMS (3 pg TBT as Sn). A comparison of the uncertainties associated with both methods indicates superior precision of the GC approach. This is related to the better reproducibility of the peak integration, which affects the isotope ratio measurements used for IDMS. The accuracy of the ASE method combined with HPLC-ICP-IDMS was demonstrated during the international interlaboratory comparison P-18 organised by the Comité Consultatif pour la Quantité de Matière (CCQM). The results obtained by GC-ICP-IDMS for a newly opened bottle of PACS-2 were 1087+/-77 ng g(-1) Sn for DBT and 876+/-51 ng g(-1) Sn for TBT (expanded uncertainties with a coverage factor of 2), which are in good agreement with the certified values of 1090+/-150 ng g(-1) Sn and 980+/-130 ng g(-1) Sn, respectively. PMID:12879193

  1. Multiple Equilibria and Interfirm Macro-Externality: An Analysis of Sluggish Real Adjustment

    OpenAIRE

    Yew-Kwang Ng; Ying Wu

    2004-01-01

    In an imperfectly competitive economy, a continuum of equilibria at the firm level exists under certain analytical conditions (Ng 1986). Extending the earlier analysis of a representative firm, this paper shows that even if the condition for a continuum of equilibria is not exactly satisfied, the factors of price-adjustment costs, interfirm heterogeneity, and macro-externality can cause the economy to be stuck at the quasi macroequilibria after aggregate demand experiences a contractionary sh...

  2. Preparation of Functional Magnetic Nanoparticles and its Application in Diagnostic Analysis

    Institute of Scientific and Technical Information of China (English)

    Qiong CHENG; Tu Zhi PENG; Ai Li LIU

    2005-01-01

    The magnetic nanoparticles modified with carboxyl functional group were synthesized and characterized. These nanoparticles covalently bound with hepatitis B surface antibody (HBsAb), were used to detect hepatitis B surface antigen (HBsAg) in immunovoltammetry. The detection limit was found to be 0.06 ng/mL, which is much higher than that of enzyme-linked immunosorbent assay (ELISA) used in clinical analysis.

  3. Сеть DISN как прототип сети связи гражданской обороны NG112

    OpenAIRE

    Шнепс-Шнеппе М.А.; Селезнев С.П.; Куприяновский В.П.

    2016-01-01

    Настоящая работа посвящена обсуждению стратегии развития телекоммуникаций в мире и поощрению отечественных разработок. В статье ставится задача – воспользоваться методическими материалами по оборонной сети DISN для разработки российской сети связи гражданской обороны нового поколения NG112 (NG – Next Generation). Показаны трудности, которые имеются на пути перехода от коммутации каналов (КК) к коммутации пакетов (КП): на правительственной сети сохраняются каналы ISDN, не определены сроки отка...

  4. A single laboratory-validated LC-MS method for the analysis of tulathromycin residues in bison and deer sera and selected tissues of white-tailed deer.

    Science.gov (United States)

    Boison, Joe O; Bachtold, Kali; Matus, Johanna; Alcorn, Jane; Woodbury, Murray

    2016-05-01

    The performance characteristics of a newly developed liquid chromatography-mass spectrometry (LC-MS) method were validated and demonstrated to be fit for purpose in a pharmacokinetic and tissue depletion study of white-tailed deer and bison. Tulathromycin was extracted from bison and deer sera with acetonitrile or trifluoroacetic acid and K2 HPO4 (pH 6.8) buffer solution and cleaned up on a conditioned Bond-Elut cartridge. Tulathromycin, retained on the cartridge; it was eluted with methanol containing 2% formic acid, dried, re-constituted in methanol/1% formic acid, and analyzed by LC-MS. The limit of quantification (LOQ) of the method was 0.6 ng/mL in serum and 0.6 ng/g in tissue with RSDs ≤ 10% and accurate over the linear calibration range of 0.8-100 ng/mL for bison serum, 0.6-50 ng/mL for deer serum, 100-2500 ng/g for deer muscle tissue, and 500-5000 ng/g for deer lung tissue, all with coefficients of determination, r(2) ≥0.99. The validated method was used to quantify the concentration of tulathromycin residues in serum of bison and deer and selected tissue (lung and muscle tissue) samples obtained from 10 healthy, white-tailed deer that were administered the therapeutic dose approved for cattle (i.e., a single 2.5 mg/kg subcutaneous injection of tulathromycin in the neck). The deer were included in a tulathromycin drug depletion study. © 2016 Her Majesty the Queen in Right of Canada. Drug Testing and Analysis © 2016 John Wiley & Sons, Ltd. PMID:27443215

  5. Removal of halogenated emerging contaminants from water by nitrogen-doped graphene decorated with palladium nanoparticles: Experimental investigation and theoretical analysis.

    Science.gov (United States)

    Li, Lei; Gong, Li; Wang, Yi-Xuan; Liu, Qi; Zhang, Jie; Mu, Yang; Yu, Han-Qing

    2016-07-01

    The removal performance and mechanisms of halogenated emerging contaminants from water by palladium decorated nitrogen-doped graphene (Pd/NG) were investigated in this study. For comparison, three catalysts of Pd/NG, palladium decorated graphene (Pd/G) and commercial Pd/C were initially explored to degrade tetrabromobisphenol A (TBBPA). After that, the influence of various environmental parameters on TBBPA removal by the Pd/NG catalyst was evaluated. Moreover, both Langmuir-Hinshelwood model and density functional theory (DFT) were adopted to theoretically elucidate the adsorption and the activation of TBBPA on the catalyst. The results show that the apparent rate constant of TBBPA dehalogenation was increased by 26.7% and 39.0% in the presence of the Pd/NG catalyst compared to the Pd/G and Pd/C ones. Higher temperature, catalyst dosage and alkaline conditions resulted in the enhancement of TBBPA dehalogenation by the Pd/NG catalyst, while humic acid in the solution had a negatively effect on the transformation of TBBPA. The corresponding rate constant value exhibited a 2.1- and 1.8-fold increase with the rise of temperature from 298 to 328 K and initial pH from 6.5 to 9.0, respectively. On the contrary, the rate constant was decreased by 78.9% in the presence of 15 mg L(-1) humic acid. Theoretical analysis revealed that both adsorption and activation processes of TBBPA on the Pd/NG catalyst were enhanced through the N doping into graphene framework. PMID:27107141

  6. Multi-element characterization of silicon nitride powders by instrumental and radiochemical neutron activation analysis

    Energy Technology Data Exchange (ETDEWEB)

    Franek, M.; Krivan, V. (Ulm Univ. (Germany). Sektion Analytik und Hoechstreinigung)

    1992-07-15

    An optimized instrumental neutron activation analysis method was applied to the comprehensive trace characterization of good- and high- purity silicon nitride powders of different origins. Experimental modes are given for 55 elements leading to limits of detection below 1 ng g[sup -] [sup 1] for 28 elements, between 1 and 100 ng g[sup -1] for 19 elements and higher than 100 ng g[sup -1] for 8 elements. For the removal of the radionuclides [sup 140]La, [sup 182]Ta and [sup 187]W, which cause the major activity in certain types of materials, radiochemical procedures based in cation exchange from 2 M HCl and anion exchange from 2 M HF were developed. [sup 64]Cu was selectively extracted with dithizone from 10 M HF for counting the 511-keV line. By radiochemical neutron activation analysis, the limits of detection were improved by up to three orders of magnitude. Comparison with results obtained by inductively coupled plasma (ICP) atomic emission spectrometry and ICP mass spectrometry shows satisfactory agreement and demonstrates the advantages of neutron activation analysis especially when low elements contents are to be determined. (author). 30 refs.; 2 figs.; 6 tabs.

  7. A multi-residue method for the analysis of pesticides and pesticide degradates in water using HLB solid-phase extraction and gas chromatography-ion trap mass spectrometry

    Science.gov (United States)

    Hladik, M.L.; Smalling, K.L.; Kuivila, K.M.

    2008-01-01

    A method was developed for the analysis of over 60 pesticides and degradates in water by HLB solid-phase extraction and gas-chromatography/mass spectrometry. Method recoveries and detection limits were determined using two surface waters with different dissolved organic carbon (DOC) concentrations. In the lower DOC water, recoveries and detection limits were 80%-108% and 1-12 ng/L, respectively. In the higher DOC water, the detection limits were slightly higher (1-15 ng/L). Additionally, surface water samples from four sites were analyzed and 14 pesticides were detected with concentrations ranging from 4 to 1,200 ng/L. ?? 2008 Springer Science+Business Media, LLC.

  8. Alan Zayıflatmasız ve Alan Zayıflatmalı, Akı ve Moment Döngülü Akım Referanslı Pwm İndirekt İleri Beslemeli Vektör Kontrolü

    OpenAIRE

    AYTAC KORKMAZ, Sevcan; KURUM, Hasan

    2013-01-01

    Bu makalede, alan zayıflamasız ve alan zayıflatmalı, akı ve moment döngülü akım referanslı PWM indirekt ileri beslemeli vektör kontrolü yapılmıştır. Alan zayıflatmasız ve alan zayıflatmalı PWM indirekt ileri beslemeli vektör kontrolü çalışması yapıldığında şekillerden de anlaşılacağı üzere farklılıklar vardır. Tanıtılan asenkron motorun dinamik modeli ve kontrol yapısının araştırılması için benzetim programı yapılmıştır. Bu programlar için MATLAB 6.5‘ dan yararlanılmıştır. ...

  9. Evaluation of natural gas supply options for south east and central Europe. Part 1: Indicator definitions and single indicator analysis

    International Nuclear Information System (INIS)

    The need for diversification of energy sources is an immanent goal in long term energy strategy. In particular, this is of great importance for the natural gas supply. In this respect, evaluation and assessment of potential natural gas resources and their relation to consumers is of great importance. The natural gas supply in Europe is one of the main issues of European energy strategy to be followed in the future. In particular, the natural gas supply in the southeast countries is important. This paper provides a framework for understanding how much natural gas is available for use in south east and central Europe as well as the links to the recent supply of natural gas and its transport. The analysis is focused on evaluation of the potential routes for natural gas supply to the south east and central European countries. The potential options included in this analysis are the Yamal Route; Nabucco Route; West Balkan Route; LNG NEUM Route and Gas by Wire Route. In this analysis, attention is focused on the following indicators for assessment of potential options: environmental indicator; NG cost indicator; NG transport and royalty indicator; investment indicator; and NG demand indicator. The first part of this paper is devoted to the definition of the indicators and to single indicator analysis. (author)

  10. Integrating Paper Chromatography with Electrochemical Detection for the Trace Analysis of TNT in Soil

    Directory of Open Access Journals (Sweden)

    Patrick Ryan

    2015-07-01

    Full Text Available We report on the development of an electrochemical probe for the trace analysis of 2,4,6-trinitrotoluene (TNT in soil samples. The probe is a combination of graphite electrodes, filter paper, with ethylene glycol and choline chloride as the solvent/electrolyte. Square wave chromatovoltammograms show the probes have a sensitivity for TNT of 0.75 nA/ng and a limit of detection of 100 ng. In addition, by taking advantage of the inherent paper chromatography step, TNT can be separated in both time and cathodic peak potential from 4-amino-dinitrotolene co-spotted on the probe or in soil samples with the presence of methyl parathion as a possible interferent.

  11. Automatic recognition of polychlorinated biphenyls in gas-chromatographic/mass spectrometric analysis

    International Nuclear Information System (INIS)

    A computer code for automatic recognition of mass spectra of polychlorinated biphenyls (PCBs) has been developed and used as a specific PCB detector in the gas-chromatographic/mass spectrometric analysis. The recognition is based on numerical features to be extracted from the mass spectrum. The code is in Fortran. The result of a classification are the so-called classification chromatograms for the particular groups of PCBs of equal chlorine number. The practical application has been tested on water- and waste oil samples, with PCBs added. The sensitivity is 0,5-1 ng for separate PCB components and 5-20 ng for technical PCD mixtures. 59 refs., 50 figs., 5 tabs. (qui)

  12. Environmental monitoring for uranium and neptunium at Yucca Mountain using Epithermal Neutron Activation Analysis

    International Nuclear Information System (INIS)

    Epithermal Neutron Activation Analysis (ENAA) is investigated as an analysis method for uranium and neptunium in environmental samples from Yucca Mountain. The design and construction of a facility for this technique are described. Theoretical improvement in sensitivity for ENAA over thermal NAA (TNAA) is discussed and compared to experimental results for different sample types. Uranium is analyzed in eight different sample matrices, including samples from Yucca Mountain. Neptunium has been studied only in AGV-1 Granite. As predicted by theory, uranium shows a high experimental sensitivity improvement factor (average = 7.76), while neptunium has a factor of only 0.49. Detection limits for uranium using ENAA range from 6 to 52 ppb by weight (2.6 to 17 ng in sample) for the different matrices. Neptunium shows a detection limit of 57 ppb by weight (6.2 ng in sample) in AGV-1 Granite using ENAA. Using TNAA, neptunium can be analyzed to 35 ppB by weight (3.4 ng in sample)

  13. Trace analysis of multi-class pesticide residues in Chinese medicinal health wines using gas chromatography with electron capture detection

    Science.gov (United States)

    Kong, Wei-Jun; Liu, Qiu-Tao; Kong, Dan-Dan; Liu, Qian-Zhen; Ma, Xin-Ping; Yang, Mei-Hua

    2016-02-01

    A method is described for multi-residue, high-throughput determination of trace levels of 22 organochlorine pesticides (OCPs) and 5 pyrethroid pesticides (PYPs) in Chinese medicinal (CM) health wines using a QuEChERS (quick, easy, cheap, effective, rugged, and safe) based extraction method and gas chromatography-electron capture detection (GC-ECD). Several parameters were optimized to improve preparation and separation time while still maintaining high sensitivity. Validation tests of spiked samples showed good linearities for 27 pesticides (R = 0.9909-0.9996) over wide concentration ranges. Limits of detection (LODs) and quantification (LOQs) were measured at ng/L levels, 0.06-2 ng/L and 0.2-6 ng/L for OCPs and 0.02-3 ng/L and 0.06-7 ng/L for PYPs, respectively. Inter- and intra-day precision tests showed variations of 0.65-9.89% for OCPs and 0.98-13.99% for PYPs, respectively. Average recoveries were in the range of 47.74-120.31%, with relative standard deviations below 20%. The developed method was then applied to analyze 80 CM wine samples. Beta-BHC (Benzene hexachloride) was the most frequently detected pesticide at concentration levels of 5.67-31.55 mg/L, followed by delta-BHC, trans-chlordane, gamma-BHC, and alpha-BHC. The validated method is simple and economical, with adequate sensitivity for trace levels of multi-class pesticides. It could be adopted by laboratories for this and other types of complex matrices analysis.

  14. Analysis of organophosphate flame retardant diester metabolites in human urine by liquid chromatography electrospray ionisation tandem mass spectrometry.

    Science.gov (United States)

    Van den Eede, Nele; Neels, Hugo; Jorens, Philippe G; Covaci, Adrian

    2013-08-16

    A new analytical method was developed for the determination of dialkyl and diaryl phosphates (DAPs), which are metabolites of organophosphate triesters (PFRs), in human urine. Target DAPs included dibutyl phosphate (DBP), diphenyl phosphate (DPHP), bis(2-butoxyethyl) phosphate (BBOEP), bis(2-chloroethyl) phosphate (BCEP), bis(1-chloro-2-propyl) phosphate (BCPP), and bis(1,3-dichloro-2-propyl) phosphate (BDCIPP). Sample preparation was based on solid phase extraction using a weak anion exchange sorbent (Oasis WAX). Although several instrumental techniques have been tested, best results were obtained with reversed phase liquid chromatography-negative electrospray ionisation tandem mass spectrometry (LC-ESI-MS/MS) taking the total analysis time into account. Method accuracy at 3ng/mL in pooled urine ranged between 69 and 119% (recovery), while inter-day imprecision (as relative standard deviation) was sensitivity for BCEP, BCIPP, and BDCIPP, their respective mLOQs being 0.1, 0.06, 0.02ng/mL, compared to 1.2, 3.7, and 0.5ng/mL by LC-MS/MS. A set of urine samples from volunteers was analysed, in which DPHP was the major DAP metabolite. A significant increase of DPHP levels was observed in the group of smokers (geometric mean of 1.55ng/mL) compared to the non-smokers (geometric mean of 0.88ng/mL). Metabolic transformation of triphenyl phosphate to DPHP by metabolic enzymes induced in smokers could be an explanation for this observation. PMID:23849782

  15. Evaluation of the determination of iodine in drinking well water by neutron activation analysis and separation by isotope exchange

    International Nuclear Information System (INIS)

    The determination of total iodine in drinking and well water by neutron activation analysis and separation of 128I(T=25.0 min) by isotope exchange has been examined at the 5-15 ng.ml-1 level. Using the Lazy Suzan of a Triga Mark-II reactor, the capacity is ≤5 samples per hour at a precision of 15-20% per aliquot and no apparent systematic bias. (author) 6 refs.; 1 tab

  16. High-performance liquid chromatographic analysis of ampicillin.

    Science.gov (United States)

    Tsuji, K; Robertson, J H

    1975-09-01

    A high-pressure liquid chromatographic method for the analysis of ampicillin is described. The method uses a 1-m long stainless steel column packed with anionic exchange resin, with a mobile phase of 0.02 M NaNO3 in 0.01 M pH 9.15 borate buffer at a flow rate of 0.45 ml/min. The degradation products of ampicillin, penicillenic and penicilloic acids of ampicillin, can be separated and quantitated in less than 12 min of chromatographic time. The relative standard deviation for the analysis of ampicillin is less than 1%, and the method is sensitive to approximately 20 ng of ampicillin/sample injected. The method was applied to the analysis of various pharmaceutical preparations of ampicillin. It is also applicable, with a slight modification, for the analysis of penicillins G and V. PMID:1185575

  17. Development of a non-chromatographic method for the speciation analysis of inorganic antimony in mushroom samples by hydride generation atomic fluorescence spectrometry

    Science.gov (United States)

    Sousa Ferreira, Hadla; Costa Ferreira, Sergio Luis; Cervera, M. Luisa; de la Guardia, Miguel

    2009-06-01

    A simple and sensitive method has been developed for the direct determination of toxic species of antimony in mushroom samples by hydride generation atomic fluorescence spectrometry (HG AFS). The determination of Sb(III) and Sb(V) was based on the efficiency of hydride generation employing NaBH 4, with and without a previous KI reduction, using proportional equations corresponding to the two different measurement conditions. The extraction efficiency of total antimony and the stability of Sb(III) and Sb(V) in different extraction media (nitric, sulfuric, hydrochloric, acetic acid, methanol and ethanol) were evaluated. Results demonstrated that, based on the extraction yield and the stability of extracts, 0.5 mol L - 1 H 2SO 4 proved to be the best extracting solution for the speciation analysis of antimony in mushroom samples. The limits of detection of the developed methodology were 0.6 and 1.1 ng g - 1 for Sb(III) and Sb(V), respectively. The relative standard derivation was 3.8% (14.7 ng g - 1 ) for Sb(V) and 5.1% (4.6 ng g - 1 ) for Sb(III). The recovery values obtained for Sb(III) and Sb(V) varied from 94 to 106% and from 98 to 105%, respectively. The method has been applied to determine Sb(III), Sb(V) and total Sb in five different mushroom samples; the Sb(III) content varied from 4.6 to 11.4 ng g - 1 and Sb(V) from 14.7 to 21.2 ng g - 1 . The accuracy of the method was confirmed by the analysis of a certified reference material of tomato leaves.

  18. Solid-phase extraction in combination with dispersive liquid-liquid microextraction and ultra-high performance liquid chromatography-tandem mass spectrometry analysis: the ultra-trace determination of 10 antibiotics in water samples.

    Science.gov (United States)

    Liang, Ning; Huang, Peiting; Hou, Xiaohong; Li, Zhen; Tao, Lei; Zhao, Longshan

    2016-02-01

    A novel method, solid-phase extraction combined with dispersive liquid-liquid microextraction (SPE-DLLME), was developed for ultra-preconcentration of 10 antibiotics in different environmental water samples prior to ultra-high performance liquid chromatography-tandem mass spectrometry detection. The optimized results were obtained as follows: after being adjusted to pH 4.0, the water sample was firstly passed through PEP-2 column at 10 mL min(-1), and then methanol was used to elute the target analytes for the following steps. Dichloromethane was selected as extraction solvent, and methanol/acetonitrile (1:1, v/v) as dispersive solvent. Under optimal conditions, the calibration curves were linear in the range of 1-1000 ng mL(-1) (sulfamethoxazole, cefuroxime axetil), 5-1000 ng mL(-1) (tinidazole), 10-1000 ng mL(-1) (chloramphenicol), 2-1000 ng mL(-1) (levofloxacin oxytetracycline, doxycycline, tetracycline, and ciprofloxacin) and 1-400 ng mL(-1) (sulfadiazine) with a good precision. The LOD and LOQ of the method were at very low levels, below 1.67 and 5.57 ng mL(-1), respectively. The relative recoveries of the target analytes were in the range from 64.16% to 99.80% with relative standard deviations between 0.7 and 8.4%. The matrix effect of this method showed a great decrease compared with solid-phase extraction and a significant value of enrichment factor (EF) compared with dispersive liquid-liquid microextraction. The developed method was successfully applied to the extraction and analysis of antibiotics in different water samples with satisfactory results. PMID:26780712

  19. Ultratrace characterization of AlSiCu sputter targets for Th, U and 35 other elements by neutron activation analysis

    International Nuclear Information System (INIS)

    Instrumental and radiochemical neutron activation analysis has been applied to a comprehensive trace characterization of AlSiCu sputter targets. By instrumental neutron activation analysis via long-lived indicator radionuclides, up to 33 elements were assayed with detection limits between 0.01 and 200 ng x g-1. The high activity of 64Cu and 24Na produced from the matrix significantly limits the instrumental performance via short- and medium-lived indicator dionuclides. For this reason, a radiochemical separation was developed based on adsorption of 24Na on hydrated antimony pentoxide and extraction of 64Cu by diethylammonuim diethyldithiocarbamate from HCl medium. By this radiochemical method, As, Ga, K, La, Mn, Mo, Re, Sb, U and W could be assayed via medium-lived radionuclides and the achievable limits of detection were between 0.1 and 25 ng x g-1. Further improvement of detection limits for U and Th was achieved by a selective radiochemical separation of 239Np and 233Pa on a Dowex 1 x 8 column in HF and HF/NH4F medium providing limits of detection for U and Th of 0.06 and 0.02ng x g-1 respectively. These techniques were applied to the analysis of two AlSiCu sputter target materials. Results are compared with those of glow discharge mass spectrometry. (author). 24 refs., 4 figs., 5 tabs

  20. Molecularly imprinted polymer cartridges coupled on-line with high performance liquid chromatography for simple and rapid analysis of human insulin in plasma and pharmaceutical formulations.

    Science.gov (United States)

    Moein, Mohammad Mahdi; Javanbakht, Mehran; Akbari-adergani, Behrouz

    2014-04-01

    In this paper, a novel method is described for automated determination of human insulin in biological fluids using principle of sequential injection on a molecularly imprinted solid-phase extraction (MISPE) cartridge as a sample clean-up technique combined with high performance liquid chromatography (HPLC). The water-compatible molecularly imprinted polymers (MIPs) were prepared using methacrylic acid as a functional monomer, ethylene glycol dimethacrylate as a cross-linker, chloroform as a porogen and insulin as a template molecule. The imprinted polymers were then employed as the solid-phase extraction sorbent for on-line extraction of insulin from human plasma samples. To achieve the best condition, influential parameters on the extraction efficiency were thoroughly investigated. Rapid and simple analysis of the hormone was successfully accomplished through the good selectivity of the prepared sorbent coupled with HPLC. Limits of detection (LOD) and quantification (LOQ) of 0.2 ng mL(-1), 0.7 ng mL(-1), and 0.03 ng mL(-1), 0.1 ng mL(-1) were obtained in plasma and urine respectively. The obtained data exhibited the great recoveries for extraction of insulin from human plasma and pharmaceutical samples, higher than 87%. PMID:24607106

  1. Trace analysis of trimethoprim and sulfonamide, macrolide, quinolone, and tetracycline antibiotics in chlorinated drinking water using liquid chromatography electrospray tandem mass spectrometry

    Science.gov (United States)

    Ye, Z.; Weinberg, H.S.; Meyer, M.T.

    2007-01-01

    A multirun analytical method has been developed and validated for trace determination of 24 antibiotics including 7 sulfonamides, 3 macrolides, 7 quinolones, 6 tetracyclines, and trimethoprim in chlorine-disinfected drinking water using a single solid-phase extraction method coupled to liquid chromatography with positive electrospray tandem mass spectrometry detection. The analytes were extracted by a hydrophilic-lipophilic balanced resin and eluted with acidified methanol (0.1% formic acid), resulting in analyte recoveries generally above 90%. The limits of quantitation were mostly below 10 ng/L in drinking water. Since the concentrated sample matrix typically caused ion suppression during electrospray ionization, the method of standard addition was used for quantitation. Chlorine residuals in drinking water can react with some antibiotics, but ascorbic acid was found to be an effective chlorine quenching agent without affecting the analysis and stability of the antibiotics in water. A preliminary occurrence study using this method revealed the presence of some antibiotics in drinking waters, including sulfamethoxazole (3.0-3.4 ng/L), macrolides (1.4-4.9 ng/L), and quinolones (1.2-4.0 ng/L). ?? 2007 American Chemical Society.

  2. Analysis of the injection process in direct injected natural gas engines. Part 1: Study of unconfined and in-cylinder plume behavior

    Energy Technology Data Exchange (ETDEWEB)

    Jennings, M.J.; Jeske, F.R. (Ricardo North America, Burr Ridge, IL (United States))

    1994-10-01

    A study of natural gas (NG) direct injection (DI) processes has been performed using multidimensional computational fluid dynamics analysis. The purpose was to improve the understanding of mixing in DI NG engines. Calculations of injection into a constant-volume chamber were performed to document unconfined plume behavior. A full three-dimensional calculation of injection into a medium heavy-duty diesel engine cylinder was also performed to study plume behavior in engine geometries. The structure of the NG plume is characterized by a core of unmixed fuel confined to the near-field of the jet. This core contains the bulk of the unmixed fuel and is mixed by the turbulence generated by the jet shear layer. The NG plume development in the engine is dominated by combustion chamber surface interactions. A Coanda effect causes plume attachment to the cylinder head, which has a detrimental impact on mixing. Unconfined plume calculations with different nozzle hole sizes demonstrate that smaller nozzle holes are more effective at mixing the fuel and air.

  3. The use of hogdahl convention k0 neutron activation analysis (NAA) standardization method and atomic absortion spectroscopy (ASS) for determination of toxic elements in foodstuffs

    International Nuclear Information System (INIS)

    Copper, cadmium, tin, chromium, arsenic, antimony, vanadium and mercury contents were determined in various foodstuffs (Mussa paradisiaca (plantains), Manihot esculentus (cassavas), Vantosoma sagittifolium (cocoyam). Vantosoma sagittifolium leaves (kontomire), Lycopersicum esculentus (tomatoes), Capsicum species (peppers), Solanum melongena (garden eggs), Nbelmoschus esculentus (okro), and Colocasia esculenta (kooko or taro)) produced in the Wassa West District, Ghana. These plants are the basis of human nutrition in the study area. These elements were determined using Hogdahl convention k0 Instrumental Neutron Activation Analysis (NAA) Standardization Method and Atomic Absorption Spectroscopy (AAS). The elements in the various foodstuffs and their concentration ranges were Cu (16.87-180.06) mg/kg, As (2.68-9.84) µg/g, Cd (0.63-5.64) µg/g, Hg (0.01-67) ng/g, Cr (0.03-3.66) µg/g, Sb (1.1- 18.6) ng/g, Sn (3.4-58.4) ng/g, and V (12-99) ng/g. The study showed that there are high levels of toxic elements in the foodstuffs grown in the mining areas as compared to that of the non-mining area (i.e., control area). This could be attributed to gold mines pollution. Compared to the World Health Organization (WHO) permissible levels of toxic elements in foods, Cu, Cr and Hg were above the permissible levels whereas the concentrations of As, Cd. Sb, Sn and V fall within the permissible levels. (au)

  4. Estacionalidad de egresos por neumonía en el Servicio de Pediatría del Hospital "Maximiliano Peralta" y su asociación con la migración indígena Ngöbe Buglé

    Directory of Open Access Journals (Sweden)

    María de la Paz Barboza- Argüello

    2005-04-01

    Full Text Available Objetivo: El presente estudio pretende determinar la magnitud del incremento en los casos de neumonía a finales de 2002, y la posible asociación de éste con la migración estacional de indígenas Ngöbe Buglé, a la zona de Los Santos, durante la época de cosecha de café. Metodología: Se analizaron los egresos por neumonía (Clasificación internacional de las CIE10= J18 del Servicio de Pediatría, del Hospital Max Peralta (HMP durante 1997-2003. Se revisaron los expedientes de los egresos del Servicio de Pediatría, por cuadros respiratorios (CIE10= J00-J99 referidos de la zona de Los Santos durante enero 2002-febrero 2003. Se estableció una definición de caso que identificara los pacientes indígenas con neumonía. Resultados: Durante el cuatrimestre octubre-enero de los años 1997 a 2002, el número de egresos por neumonía fue en promedio 1,7 veces mayor que el de los cuatrimestres febrero- mayo y junio-septiembre. En este cuatrimestre (octubre-enero, alrededor del 50% de los egresos correspondieron a pacientes referidos de los Santos. Durante enero 2002 y febrero 2003, los pacientes indígenas provenientes de la zona de Los Santos representaron el 40% (66/163 de todos los egresos por neumonía. La distribución de ellos se concentra (99% en los meses cuando ocurre la cosecha de café (noviembre-febrero. Conclusión: Existe un comportamiento estacional de los egresos por neumonía en el Servicio de Pediatría del HMP, durante 1997-2002. La estacionalidad es dada por población indígena migrante durante la época de café

  5. Quantitative analysis of mitragynine in human urine by high performance liquid chromatography-tandem mass spectrometry.

    Science.gov (United States)

    Lu, Shijun; Tran, Buu N; Nelsen, Jamie L; Aldous, Kenneth M

    2009-08-15

    Mitragynine is the primary active alkaloid extracted from the leaves of Mitragyna speciosa Korth, a plant that originates in South-East Asia and is commonly known as kratom in Thailand. Kratom has been used for many centuries for their medicinal and psychoactive qualities, which are comparable to that of opiate-based drugs. Kratom abuse can lead to a detectable content of mitragynine residue in urine. Ultra trace amount of mitragynine in human urine was determined by a high performance liquid chromatography coupled to an electrospray tandem mass spectrometry (HPLC-ESI/MS/MS). Mitragynine was extracted by methyl t-butyl ether (MTBE) and separated on a HILIC column. The ESI/MS/MS was accomplished using a triple quadrupole mass spectrometer in positive ion detection and multiple reactions monitoring (MRM) mode. Ajmalicine, a mitragynine's structure analog was selected as internal standard (IS) for method development. Quality control (QC) performed at three levels 0.1, 1 and 5 ng/ml of mitragynine in urine gave mean recoveries of 90, 109, and 98% with average relative standard deviation of 22, 12 and 16%, respectively. The regression linearity of mitragynine calibration ranged from 0.01 to 5.0 ng/ml was achieved with correlation coefficient greater than 0.995. A detection limit of 0.02 ng/ml and high precision data within-day and between days analysis were obtained. PMID:19577523

  6. Analysis of zearalenone in cereal and Swine feed samples using an automated flow-through immunosensor.

    Science.gov (United States)

    Urraca, Javier L; Benito-Peña, Elena; Pérez-Conde, Concepción; Moreno-Bondi, María C; Pestka, James J

    2005-05-01

    The development of a sensitive flow-though immunosensor for the analysis of the mycotoxin zearalenone in cereal samples is described. The sensor was completely automated and was based on a direct competitive immunosorbent assay and fluorescence detection. The mycotoxin competes with a horseradish-peroxidase-labeled derivative for the binding sites of a rabbit polyclonal antibody. Control pore glass covalently bound to Prot A was used for the oriented immobilization of the antibody-antigen immunocomplexes. The immunosensor shows an IC(50) value of 0.087 ng mL(-1) (RSD = 2.8%, n = 6) and a dynamic range from 0.019 to 0.422 ng mL(-1). The limit of detection (90% of blank signal) of 0.007 ng mL(-1) (RSD = 3.9%, n = 3) is lower than previously published methods. Corn, wheat, and swine feed samples have been analyzed with the device after extraction of the analyte using accelerated solvent extraction (ASE). The immunosensor has been validated using a corn certificate reference material and HPLC with fluorescence detection. PMID:15853369

  7. Study of organohalogens in foodstuffs and environmental samples by neutron activation analysis and related techniques

    International Nuclear Information System (INIS)

    Pine needles and foodstuffs collected from Beijing, China, were analyzed by instrumental neutron activation analysis (INAA) combined with organic solvent extraction for total halogens, extractable organohalogens (EOX) and extractable persistent organohalogens (EPOX). The INAA detection limits are 50 ng, 8 ng and 3.5 ng for Cl, Br and I, respectively. The contents and distribution patterns of organohalogens in these samples are reported. EOCl accounted for 0.013-0.016% and 1.6-2.7% of the total chlorine in yogurt and apples, respectively, which suggested that chlorine in foodstuffs mainly existed as inorganic species and non-extractable organochlorines. EOCl contents in pine needles and foodstuffs were noticeably higher than those of EOBr and EOI. For pine needles, yogurt and apples, 1.6-34%, 23-58% and 29-35% of EOCl remained as extractable persistent organochlorine (EPOCl), respectively. Pine needle containing higher EOCl contents in chemical industrial and traffic hub areas indicated that chemical industries and exhaust emission from vehicle were the main sources of organochlorines in the Beijing's air. The relative proportions of the known organochlorines (such as HCHs, DDTs, chlordanes, heptachlor, HCB and PCBs) to the total EOCl and EPOCl were 0.04-1.6% and 0.7-21.5%, respectively, which implied that the identity of species of a major portion of the EOCl and EPOCl measured in pine needles was unknown. (author)

  8. Analysis of radioactive impurities in alumina and silica used for electronic materials

    International Nuclear Information System (INIS)

    It has been established that soft error of high precision electronic circuits can be induced by alpha-particles emitted from the naturally occurring radioactive impurities such as U, Th. As the electronic circuits have recently become lower dimension and higher density, these alpha-particle emitting radioactive impurities have to be strictly controlled. The aim of this study is to develop of NAA(Neutron Activation Analysis) and gamma-spectrometry to improve the analytical sensitivity(ng/g) and precision of U and Th. New NAA methods have been established using the HTS(Hydraulic Transfer System) irradiation facility which has been used to produce radioisotopes for industries and medicines instead of PTS(Pneumatic Transfer System) irradiation facility which has been used in general NAA. When the ultra-trace impurities have to be analyzed by NAA, background gamma-ray spectra induced from 222Rn and its progeny nuclides in air is serious problem. This unstable background has been eliminated or stabilized by the use of a nitrogen purging system. Ultra trace amounts of U(0.1ng/g) and Th(0.01ng/g) in alumina ball and high purity silica used for EMC could be analyzed by the use of HTS-NAA and low background gamma-spectrometry

  9. Radioimmunometric analysis of histamine in myeloproliferative syndromes

    International Nuclear Information System (INIS)

    Quantitative analysis of histamine is increasingly used in clinical haematology. The present study demonstrates the properties of and potential indication for a novel histamine radioimmunoassay (RIA) in clinical haematology. The sensitivity of this test assay corresponds to a histamine level of 0,1 to 0,5 nM, the non-specific cross reaction with endogeneous histamine metabolites appears to be less than 0,1%. The total histamine levels in the peripheral blood of healthy donors (n = 10) ranged from 10 to 100 ng/ml, the plasma histamine values from 0,02 to 0,6 ng/ml blood. Increased levels of total histamine were measured in myeloproliferative syndromes, i.e. in patients with chronic myeloid leukemia (CML)(8 of 9), myelofibrosis (OMS)(2 of 4), and polycythaemia vera (PCV)(1 of 2). An excessive increase in total histamine was observed in healthy rhesus monkeys (n = 10) treated with recombinant human interleukin-3 (rhIL-3). The total histamine value correlated with the absolute number of circulating blood basophils (correlation coefficient: 0,9). The calculated content of histamine per basophil was found to be 0,5 to 1,5 pg. Plasma histamine values in patients suffering from myeloproliferative syndromes were within the normal range. In contrast, a moderate to marked increase in plasma histamine values was observed in monkeys during IL-3 treatment. The radioimmunometric analysis of histamine clearly represents a useful new test system in clinical haematology, especially in the follow up of malignant as well as IL-3-induced myeloproliferation. (Authors)

  10. GC-MS/MS analysis of benzo(a)pyrene by ion trap tandem mass spectrometry

    International Nuclear Information System (INIS)

    The mass spectrometry using an ion trap tandem mass spectrometer has been investigated to find optimum conditions for the analysis of benzo(a)pyrene (3,4-benzpyrene). The applicability to a real soil sample was also investigated to verify the usefulness of the MS/MS (or collision induced dissociation, CID) analysis. The optimum CID condition was 1.5 and 0.45 for the RF excitation voltage and the q value, respectively. For comparison, CID and EI were applied to the analysis of a soil sample. CID analysis was more sensitive than EI analysis of the soil sample. The limit of detection (LOD) of benzo(a)pyrene was 3.18 ng mL-1 and 0.85 ng mL-1 for EI and MS/MS analysis, respectively. The precision at the soil sample for EI and CID showed relative standard deviations of 6.1% and 4.1%, respectively, and the concentrations were 168 μg kg-1 and 162 μg kg-1, respectively

  11. Mehmet Harmancı’nın Küçürek Öykülerindegündelik Kuşatma Altındaki İnsanın Varoluş Görüngüleri Existence Perspectives Of An Individual Under Daily Blockage In The Short Short Stories Of Mehmet Harmancı

    Directory of Open Access Journals (Sweden)

    Mutlu DEVECİ

    2013-03-01

    şme sürecinde değerlerini/ özünü koruyarak geleceğe taşınmalıdır. Diğerleri gibi kalmak, bireyleşememek, şey’ler dünyasında yitip gitmektir. İnsan gerçeğini tarihsel boyutuyla ele alan Mehmet Harmancı, öykülerinde, bireyin kendi oluşuna göndermede yapar. Anlam arayışı içerisindeki insanların; ölüm, aşk ve yalnızlık izlekleri etrafında eylem ve eylemsizliklerini dile getirir.Mehmet Harmancı Muhtemel Menkıbeler adlı eserinde yer alan küçürek öykülerinde, yaşamın anlamına yönelik sorgulamalarda bulunur. Türler arası geçişkenliğin dikkat çektiği bu eserde yazar, menkıbe türünün imkânlarını aşarak küçürek öykü türüne özgü bir anlatım yolu seçer. Öykülerde, şimdi’yi vareden değerler, geçmişin izdüşümünde kurulur ve gündelik kuşatma altındaki insanın varoluş görüngüleri sergilenir.Mehmet Harmancı, geleneğin izdüşümünde kurduğu Muhtemel Menkıbeler’inde moderniteyi ıskalamadan geleneği de modern olana ezdirmeden yol alır. Yazar, değerlerini yok sayarak medeniyet kurmaya çalışanlara karşı geleneği, modern dünyanın fenomenolojik temeli sayar. Bu açıdan modernite olarak kabul ettirilmeye çalışılan dünya/ yaşam yerine, temeli geleneğe dayalı bir modern dünya arzusu taşır. Muhtemel Menkıbeler’deki öykülerin pek çoğu böylesi bir ruh dünyasına aittir.Muhtemel Menkıbeler’deki öykülerde, geleneğin deneyimsel, kültürel ve nesneler belleğini başat değer olarak kabul eden yazar, yaşamın anlam arayışına yönelik sorgulamaları ile yol göstericilik özelliğini ön plana çıkarır.Çalışmada, Mehmet Harmancı’nın Muhtemel Menkıbeler adlı eserinde yer alan öyküleri analiz edilirken, menkıbe ile küçürek öykü türleri arasındaki ilişkiye dikkat çekilerek; gelenek ile modernite arasındaki insan görüngülerine yer verildi.

  12. Hair analysis as an indicator of exposure to uranium

    International Nuclear Information System (INIS)

    Medical examinations performed on four monks of a monastery in the northern Greece revealed heavy metal contamination. Hair analysis, performed by a toxicological laboratory abroad, indicated, among other, the presence of uranium. The uranium concentrations determined in a laboratory of 'Elemental Hair Analysis' indicated a uranium level that was about five times the maximum value of the reference range, which has been adopted by the measuring laboratory. After these diagnostic findings, on request of 10 monks, uranium determination in hair and urine samples was performed by means of alpha spectrometry in GAEC's laboratory. The measured uranium concentrations in hair varied from 0.15 to 2.10 mBq g-1, which correspond to 12.1 and 170 ng g-1, respectively. The uranium concentrations in urine were between 41 and 174 ng d-1. For comparison purposes, urine and non-dyed hair samples from the staff of the laboratory were analysed. Because one of the major sources of uranium intake is through drinking water, water samples were also analysed. The mean value of the uranium concentration in the two drinking water samples collected from the residence area was found to be 2.35 μg l-1. (authors)

  13. Gut fluorescence analysis of barnacle larvae: An approach to quantify the ingested food

    Science.gov (United States)

    Gaonkar, Chetan A.; Anil, Arga Chandrashekar

    2012-10-01

    Gut fluorescence analysis can provide a snapshot of ingested food and has been employed in feeding studies of various organisms. In this study we standardised the gut fluorescence method using laboratory-reared barnacle larvae (Balanus amphitrite) fed with mono-algal diet Chaetoceros calcitrans, a unicellular diatom at a cell concentration of 2 × 105 cells ml-1. The gut fluorescence of IV-VI instar nauplii was found to be 370(±12) ng chlorophyll a larva-1 and in faecal pellets it was 224(±63) ng chlorophyll a larva-1. A phaeopigment concentration in larval gut was found to be 311(±13) ng larva-1 and in faecal pellets it was 172(±61) ng larva-1. The study also analysed larval samples collected from the field during different seasons from a tropical environment influenced by monsoons (Dona Paula bay, Goa, west coast of India), with characteristic temporal variations in phytoplankton abundance and diversity. Gut fluorescence of larvae obtained during the post-monsoon season was consistently higher when compared to the pre-monsoon season, suggesting the predominance of autotrophic forms in the larval gut during the post-monsoon season. Whereas, the low gut fluorescence obtained during the pre-monsoon season indicated the ingestion of food sources other than autotrophs. Such differences observed in the feeding behaviour of larvae could be due to differential availability of food for the larvae during different seasons and indicate the capability of larvae to feed on wide range of food sources. This study shows the value of the fluorescence method in feeding studies of planktotrophic organisms and in the evaluation of ecosystem dynamics.

  14. Simultaneous analysis method for polar and non-polar ginsenosides in red ginseng by reversed-phase HPLC-PAD.

    Science.gov (United States)

    Lee, Sa-Im; Kwon, Ha-Jeong; Lee, Yong-Moon; Lee, Je-Hyun; Hong, Seon-Pyo

    2012-02-23

    The paper describes the development of a simultaneous determination method for polar and non-polar ginsenosides in red ginseng with a reversed-phase high-performance liquid chromatography-pulsed amperometric detection method. This method could be applied directly without any pretreatment steps and enabled the performance of highly sensitive analysis within 1h. The detection (S/N=3) and quantification (S/N=10) limits for the ginsenosides ranged 0.02-0.10 ng and 0.1-0.3 ng, respectively. The linear regression coefficients ranged 0.9975-0.9998. Intra- and inter-day precisions were <9.91%. The mean recoveries ranged 98.08-103.06%. The total amount of ginsenosides in the hairy root of red ginseng was higher than that in the main root. PMID:22119615

  15. Development and validation of a method for the analysis of hydroxyzine hydrochloride in extracellular solution used in in vitro preclinical safety studies.

    Science.gov (United States)

    Briône, Willy; Brekelmans, Mari; Eijndhoven, Freek van; Schenkel, Eric; Noij, Theo

    2015-11-10

    In the process of drug development, preclinical safety studies are to be performed that require the analysis of the compound at very low concentrations with high demands on the performance of the analytical methods. In the current study, a UPLC-MS/MS method was developed and validated to quantify hydroxyzine hydrochloride in an extracellular solution used in a hERG assay in concentrations ranging from 0.01 to 10μM (4.5ng/ml-4.5μg/ml). Chromatographic separation was achieved isocratically on an Acquity BEH C18 analytical column. The assay was validated at concentrations of 0.11-1.1ng/ml in end solution for hydroxyzine hydrochloride. Linearity was demonstrated over the range of concentrations of 0.06-0.17ng/ml and over the range of concentrations of 0.6-1.7ng/ml in end solution with the coefficient of correlation r>0.99. Accuracy of the achieved concentration, intra-run, and inter-run precision of the method were well within the acceptance criteria (being mean recovery of 80-120% and relative standard deviation ≤10.0%). The limit of quantification in extracellular solution was 0.09ng/ml. Hydroxyzine hydrochloride in extracellular solution proved to be stable when stored in the fridge at 4-8°C for at least 37 days, at room temperature for at least 16 days and at +35°C for at least 16 days. The analytical method was successfully applied in hERG assay. PMID:26163869

  16. An integrated approach combining chemical analysis and an in vivo bioassay to assess the estrogenic potency of a municipal solid waste landfill leachate in Qingdao.

    Directory of Open Access Journals (Sweden)

    Yufeng Gong

    Full Text Available Various adverse effects related to landfill leachate have made leachates an important issue in past decades, and it has been demonstrated that landfill leachate is an important source of environmental estrogens. In this study, we employed chemical analysis of some already evaluated estrogenic substances, in combination with a bioassay using several specific biomarkers (e.g., plasma vitellogenin and sex steroids, enzyme activity of gonad gamma-glutamyl transpeptidase, and gonadosomatic index to evaluate the estrogenic activities in outlets from different stages of the leachate treatment process. The results indicated that 5 environmental estrogens (4-t-octylphenol, bisphenol A, di-ethyl phthalate, di-n-butyl phthalate, and diethylhexyl phthalate were detected by a gas chromatography-mass spectrometry, and the concentrations in leachate samples were 6153 ng/L, 3642 ng/L, 2139 ng/L, 5900 ng/L, and 9422 ng/L, respectively. Leachate (1∶200 diluted induced the synthesis of plasma vitellogenin and led to decreased enzyme activity of gonad gamma-glutamyl transpeptidase and gonadosomatic index in male goldfish (Carassius auratus after a 28-day exposure, while increased circulating 17β-estradiol level was also observed in males exposed to treated effluent. Although the target EEs were partially removed with removal rates varying from 87.2% to 99.77% by the "membrane bioreactor+reverse osmosis+aeration zeolite biofilter" treatment process, the treated effluent is still estrogenic to fish. The method combined chemical techniques with the responses of test organisms allowing us to identify the group of estrogen-like chemicals so that we were able to evaluate the overall estrogenic effects of a complex mixture, avoiding false negative assessments.

  17. Comparative study of multi-element determination using inductively coupled plasma mass spectrometry, total reflection X-ray fluorescence spectrometry and neutron activation analysis

    International Nuclear Information System (INIS)

    The analytical capabilities of inductively coupled plasma mass spectrometry, total reflection X-ray fluorescence spectrometry and neutron activation analysis were compared. The data originated from the parallel analyses of spinach, cabbage and domestic sludge samples, which were used in inter-laboratory tests to monitor precision and accuracy. The determined concentrations range from 40 mg g-1 to 20 ng g-1 and the analytical errors from 2 to 30%. The extensive results and the reliability of the techniques are discussed. (orig.)

  18. Analysis of some egyptian cosmetic samples by using fast neutron activation analysis

    International Nuclear Information System (INIS)

    A description of our neutron generator (NG) facility for neutron activation analysis is presented. As an example, the concentration of Na, Mg, Al, Si, K, Cl, Ca and Fe elements were determined in two domestic brands of face powder by using a beam of 14 MeV neutrons. An empirical expression for detector efficiency in terms of incident gamma ray energy and the source-detector distance has been obtained for a hyper pure germanium detector (HPGe) using different standard point sources. The comparison of the calculated efficiencies and the measured values in the energy range from 59.5 to 1332.2 keV and for source-to-detector distances of 5-30 cm show the agreement between the calculated values and the measured experimental values

  19. Optimisation and validation of a HS-SPME-GC-IT/MS method for analysis of carbonyl volatile compounds as biomarkers in human urine: Application in a pilot study to discriminate individuals with smoking habits.

    Science.gov (United States)

    Calejo, Isabel; Moreira, Nathalie; Araújo, Ana Margarida; Carvalho, Márcia; Bastos, Maria de Lourdes; de Pinho, Paula Guedes

    2016-02-01

    A new and simple analytical approach consisting of an automated headspace solid-phase microextraction (HS-SPME) sampler coupled to gas chromatography-ion trap/mass spectrometry detection (GC-IT/MS) with a prior derivatization step with O-(2,3,4,5,6-pentafluorobenzyl)hydroxylamine hydrochloride (PFBHA) was developed to detect volatile carbonyl metabolites with low molecular weights in human urine. A central composite design (CCD) was used to optimise the PFBHA concentration and extraction conditions that affect the efficiency of the SPME procedure. With a sample volume of 1 mL, optimal conditions were achieved by adding 300 mg/L of PFBHA and allowing the sample to equilibrate for 6 min at 62°C and then extracting the samples for 51 min at the same temperature, using a divinylbenzene/polydimethylsiloxane (DVB/PDMS) fibre. The method allowed the simultaneous identification and quantification of 44 carbonyl compounds consisting of aldehydes, dialdehydes, heterocyclic aldehydes and ketones. The method was validated with regards to the linearity, inter- and intra-day precision and accuracy. The detection limits ranged from 0.009 to 0.942 ng/mL, except for 4-hydroxy-2-nonenal (15 ng/mL), and the quantification limits varied from 0.029 to 1.66 ng/mL, except for butanal (2.78 ng/mL), 2-butanone (2.67 ng/mL), 4-heptanone (3.14 ng/mL) and 4-hydroxy-2-nonenal (50.0 ng/mL). The method accuracy was satisfactory, with recoveries ranging from 90 to 107%. The proof of applicability of the methodology was performed in a pilot target analysis of urine samples obtained from 18 healthy smokers and 18 healthy non-smokers (control group). Chemometric supervised analysis was performed using the volatile patterns acquired for these samples and clearly showed the potential of the volatile carbonyl profiles to discriminate urine from smoker and non-smoker subjects. 5-Methyl-2-furfural (p<0.0001), 2-methylpropanal, nonanal and 2-methylbutanal (p<0.05) were identified as potentially useful

  20. Analysis for trace mercury concentration. I. Critical evaluation of current procedures. II. A proposed method for determination by instrumental neutron activation analysis

    International Nuclear Information System (INIS)

    Current methods of sample pretreatments, digestion, lyophilization and extraction, have been found to lead to considerable loss of mercury, at an initial mercury concentration of 1 μg/g, and less. Storage of solutions of mercury at concentrations of less than 1 μg/ml, in glass, Teflon and polyethylene containers, leads to losses by adsorption. Electrochemical reduction of mercury to the metal, and subsequent volatilization, is postulated as the mechanism of loss from the samples studied during lyophilization. A method of instrumental neutron activation analysis, which obviates the above pretreatments, has been developed for mercury concentrations as low as 1 ng/ml

  1. Analysis of metals and phosphorus in biodiesel B100 from different feedstock using a Flow Blurring® multinebulizer in inductively coupled plasma-optical emission spectrometry

    International Nuclear Information System (INIS)

    Highlights: • The elemental analysis of biodiesel by the proposed method is simple and fast. • Two-nozzles Flow Blurring® nebulizer allow to reduce the spectral interferences. • Two-nozzles Flow Blurring® nebulizer avoid the formation of carbon deposits. • The analysis may be carried out without any sample pretreatment. - Abstract: A simple and fast method for determining the content of Na, K, Ca, Mg, P, and 20 heavy metals in biodiesel samples with inductively coupled plasma optical emission spectrometry (ICP OES) using a two-nozzle Flow Blurring® multinebulizer prototype and on-line internal standard calibration, are proposed. The biodiesel samples were produced from different feedstock such as sunflower, corn, soybean and grape seed oils, via a base catalyst transesterification. The analysis was carried out without any sample pretreatment. The standards and samples were introduced through one of the multinebulizer nozzles, while the aqueous solution containing yttrium as an internal standard was introduced through the second nozzle. Thus, the spectral interferences were compensated and the formation of carbon deposits on the ICP torch was prevented. The determination coefficients (R2) were greater than 0.99 for the studied analytes, in the range 0.21–14.75 mg kg−1. Short-term and long-term precisions were estimated as relative standard deviation. These were acceptable, their values being lower than 10%. The LOQ for major components such as Ca, K, Mg, Na, and P, were within a range between 4.9 ng g−1 for Mg (279.553 nm) and 531.1 ng g−1 for Na (588.995 nm), and for the other 20 minor components they were within a range between 1.1 ng g−1 for Ba (455.403 nm) and 2913.9 ng g−1 for Pb (220.353 nm). Recovery values ranged between 95% and 106%

  2. Development of cancer therapy facility of HANARO and medical research in BNCT; development of the technique for boron concentration analysis

    Energy Technology Data Exchange (ETDEWEB)

    Choi, Hee Dong; Byun, Soo Hyun; Sun, Gwang Min; Kim, Suk Kwon; Kim, In Jung; Park, Chang Su [Seoul National University, Seoul (Korea)

    2002-03-01

    Objective and Necessity of the Project- Development of a boron concentration analysis facility used for BNCT. - Development of the technique for boron concentration analysis. Contents and Scopes of the Project - Construction of the boron concentration analysis facility based on PGAA. Estimation of the neutron beam characteristics. -Establishment of the technique for the boron concentration analysis. - Estimation of the reliability for the boron analysis. Results of the Project -Installation of the boron concentration analysis facility at Hanaro. - Neutron beam characteristics are the sample position (neutron flux : 7.9 x 10{sup 7} n/cm{sup 2}s, Cd-ratio : 266) Technique for the boron concentration analysis. - Boron detection sensitivity and limit (detection sensitivity : 2, 131 cps/mg-B, detection limit : 67 ng for 10,000 sec). 63 refs., 37 figs., 13 tabs. (Author)

  3. Decision analysis multicriteria analysis

    International Nuclear Information System (INIS)

    The ALARA procedure covers a wide range of decisions from the simplest to the most complex one. For the simplest one the engineering judgement is generally enough and the use of a decision aiding technique is therefore not necessary. For some decisions the comparison of the available protection option may be performed from two or a few criteria (or attributes) (protection cost, collective dose,...) and the use of rather simple decision aiding techniques, like the Cost Effectiveness Analysis or the Cost Benefit Analysis, is quite enough. For the more complex decisions, involving numerous criteria or for decisions involving large uncertainties or qualitative judgement the use of these techniques, even the extended cost benefit analysis, is not recommended and appropriate techniques like multi-attribute decision aiding techniques are more relevant. There is a lot of such particular techniques and it is not possible to present all of them. Therefore only two broad categories of multi-attribute decision aiding techniques will be presented here: decision analysis and the outranking analysis

  4. Magnetic-encoded fluorescent multifunctional nanospheres for simultaneous multicomponent analysis.

    Science.gov (United States)

    Song, Erqun; Han, Weiye; Li, Jingrong; Jiang, Yunfei; Cheng, Dan; Song, Yang; Zhang, Pu; Tan, Weihong

    2014-10-01

    In this study, magnetic-encoded fluorescent (CdTe/Fe3O4)@SiO2 multifunctional nanospheres were constructed by adjusting the initial concentration of Fe3O4 in a fabrication process based on reverse microemulsion. The resultant multifunctional nanospheres were characterized by transmission electron microscopy, X-ray diffraction measurements, fluorescence spectrophotometry, and vibrating sample magnetometry. They showed good fluorescence properties, gradient magnetic susceptibility (weak, moderate, and strong), and easy biofunctionalization for biomolecules, such as immunoglobulin G (IgG), protein, and antibody. Then the capture efficiency of the (CdTe/Fe3O4)@SiO2 nanospheres were investigated by using the fluorophore-labeled IgG-conjugated nanospheres as a model. Further studies demonstrated the ability of these (CdTe/Fe3O4)@SiO2 multifunctional nanospheres to accomplish sequentially magnetic separation, capture, and fluorescent detection for each corresponding antigen of CA125, AFP, and CEA with a detection limit of 20 KU/L, 10 ng/mL, and 5 ng/mL, respectively, from a mixed sample under a certain external magnetic field within a few minutes. The strategy of combining magnetic-encoding-based separation and fluorescence-based detection proposed in this study shows great potential to achieve easy, rapid, economical, and near-simultaneous multicomponent separation and analysis for a variety of targets such as drugs, biomarkers, pathogens, and so on. PMID:25197942

  5. Oxytetracycline analysis in honey using a specific portable analyzer

    Science.gov (United States)

    Chen, Guoying; Schwartz, Daniel; Braden, S.; Nunez, Alberto

    2007-09-01

    Oxytetracycline (OTC) residue in honey is detected using a portable analyzer designed to specifically target tetracycline (TC) drugs based on europium-sensitized luminescence (ESL). A 385 nm light emitting diode (LED) is used as the excitation source and a photomultiplier tube as the light detector. OTC is extracted from honey and cleaned up by solid phase extraction (SPE) using Strata X-WC weak cation exchange cartridges. To the eluate Eu(III) is added to form a Eu-TC chelate at pH 8.5. Efficient intrachelate energy transfer allows sensitive OTC detection at λ ex=385 nm and λ em=610 nm. After a 25-µs time delay, the ESL signal is integrated over a 25-1000 µs interval. The signal intensity reveals a linear relationship (R2=0.972) to OTC concentrations in the 10-200 ng/g range. The limit-of-detection is 6.7 ng/g with an average 5.8% relative standard deviation. The background signal corresponds to ~10 ppb. This instrumentation and method combination enables field analysis that is especially useful for beekeeping industry.

  6. A microfluidic dialysis device for complex biological mixture SERS analysis

    KAUST Repository

    Perozziello, Gerardo

    2015-08-01

    In this paper, we present a microfluidic device fabricated with a simple and inexpensive process allowing rapid filtering of peptides from a complex mixture. The polymer microfluidic device can be used for sample preparation in biological applications. The device is fabricated by micromilling and solvent assisted bonding, in which a microdialysis membrane (cut-off of 12-14 kDa) is sandwiched in between an upper and a bottom microfluidic chamber. An external frame connects the microfluidic device to external tubes, microvalves and syringe pumps. Bonding strength and interface sealing are pneumatically tested. Microfluidic protocols are also described by using the presented device to filter a sample composed of specific peptides (MW 1553.73 Da, at a concentration of 1.0 ng/μl) derived from the BRCA1 protein, a tumor-suppressor molecule which plays a pivotal role in the development of breast cancer, and albumin (MW 66.5 kDa, at a concentration of 35 μg/μl), the most represented protein in human plasma. The filtered samples coming out from the microfluidic device were subsequently deposited on a SERS (surface enhanced Raman scattering) substrate for further analysis by Raman spectroscopy. By using this approach, we were able to sort the small peptides from the bigger and highly concentrated protein albumin and to detect them by using a label-free technique at a resolution down to 1.0 ng/μl.

  7. Identification and analysis of piperitone in red wines.

    Science.gov (United States)

    Pons, Alexandre; Lavigne, Valérie; Darriet, Philippe; Dubourdieu, Denis

    2016-09-01

    The present study concerns the search for new aroma compounds associated with the flavor of aged and prematurely aged red wines. Sensory descriptive analysis associated with gas chromatography-olfactometry was first performed to find specific odoriferous zones. One of the zones, reminiscent of mint, was found in red wines with a prune flavor. Thanks to several analytical approaches including preparative gas chromatography, p-menth-1-en-3-one (piperitone), has been identified in red wines. The olfactory detection threshold of this terpene reminiscent of mint was 0.9μg/L in model hydroalcoholic solution and 70μg/L in red wines. Chiral multidimensional GC-MS was used to show that piperitone was present mainly in the (R) form in red wines. The concentration of piperitone in aged red wines ranged from a few ng/L to 435ng/L, but the level was not linked to the age of the wine nor to its premature evolution. PMID:27041315

  8. Analysis of the uranium isotope ratio in black rain from the Hiroshima A-bomb. Analytical precision and future research

    International Nuclear Information System (INIS)

    We examined the performance of ICP quadrupole mass spectrometry regarding U-234, 235 and 238 isotope ratio measurement in nano-gram uranium samples, thorough analysis of fourteen soil samples from a nuclear test site. The study was motivated by the need to analyze the minor uranium isotopes in exhibitions in Hiroshima Peace Memorial Museum, with minimum consumption of precious historic samples. The comparison of the obtained U-235/U-238 and U-234/U-238 data between our group and a national laboratory in US showed that the relative discrepancy was <0.5% and <2% when 16-40 ng uranium was used for the analysis. (author)

  9. The determination of natural uranium in human tissues by recovery corrected kinetic phosphorescence analysis

    International Nuclear Information System (INIS)

    Two typical methods used for the determination of uranium in human autopsy tissues are kinetic phosphorescence analysis (KPA) and alpha-spectrometry, both of which have significant limitations and advantages. KPA is limited because of the amount of sample used (1-10 ml for sample digestion followed by one ml KPA aliquots), no isotopic information is provided, phosphorescence degradation by salts in solution, and even more importantly, it does not provide chemical recovery information. For samples with sub ng uranium concentrations per g of inorganic material, preconcentration is necessary, which may require chemical recovery (other than simple evaporation). While alpha-spectrometry has very good radiometric detection limits for 238U, the very long half-life of 238U (4.468 x109 y) restricts its mass detection limit (27 ng). KPA, on the other hand, has a detection limit three orders of magnitude lower (0.02 ng) for natural uranium. A recovery corrected method for the determination of natural uranium in human tissues was developed combining preconcentration of human tissues dissolved in 6M HCl by anion exchange with alpha-spectrometry and kinetic phosphorescence analysis, utilizing 232U as a tracer. Solution aliquots containing up to 6 g of bone ash were preconcentrated for KPA measurement thereby allowing the use of up to 25% of the original sample solution weight for analysis by KPA. The radiochemical yield of 232U was determined by alpha-spectrometry and the uranium content was determined by KPA. The mean radiochemical yields obtained for human tissue samples range from 65% to 106% with a mean of 85%±8%. (author)

  10. Array comparative genomic hybridization analysis of Trichoderma reesei strains with enhanced cellulase production properties

    Directory of Open Access Journals (Sweden)

    Penttilä Merja

    2010-07-01

    Full Text Available Abstract Background Trichoderma reesei is the main industrial producer of cellulases and hemicellulases that are used to depolymerize biomass in a variety of biotechnical applications. Many of the production strains currently in use have been generated by classical mutagenesis. In this study we characterized genomic alterations in high-producing mutants of T. reesei by high-resolution array comparative genomic hybridization (aCGH. Our aim was to obtain genome-wide information which could be utilized for better understanding of the mechanisms underlying efficient cellulase production, and would enable targeted genetic engineering for improved production of proteins in general. Results We carried out an aCGH analysis of four high-producing strains (QM9123, QM9414, NG14 and Rut-C30 using the natural isolate QM6a as a reference. In QM9123 and QM9414 we detected a total of 44 previously undocumented mutation sites including deletions, chromosomal translocation breakpoints and single nucleotide mutations. In NG14 and Rut-C30 we detected 126 mutations of which 17 were new mutations not documented previously. Among these new mutations are the first chromosomal translocation breakpoints identified in NG14 and Rut-C30. We studied the effects of two deletions identified in Rut-C30 (a deletion of 85 kb in the scaffold 15 and a deletion in a gene encoding a transcription factor on cellulase production by constructing knock-out strains in the QM6a background. Neither the 85 kb deletion nor the deletion of the transcription factor affected cellulase production. Conclusions aCGH analysis identified dozens of mutations in each strain analyzed. The resolution was at the level of single nucleotide mutation. High-density aCGH is a powerful tool for genome-wide analysis of organisms with small genomes e.g. fungi, especially in studies where a large set of interesting strains is analyzed.

  11. Trace analysis measurements in high-purity aluminium by means of radiochemical neutron and proton activation analysis

    International Nuclear Information System (INIS)

    The aim of the study consisted in the development of efficient radiochemical composite processes and activation methods for the multi-element determination of traces within the lower ng range in high-purity aluminium. More than 50 elements were determined with the help of activation with reactor neutrons; the selective separation of matrix activity (adsorption with hydrated antimony pentoxide) led to a noticeable improvement of detectability, as compared with instrumental neutron activation analysis. Further improvements were achieved with the help of radiochemical group separations in ion exchangers or with the help of the selective separation of the pure beta-emitting elements. Over 20 elements up to high atomic numbers were determined by means of activating 13 MeV protons and 23 Me protons. In this connection, improvements of the detection limit by as a factor of 10 were achieved with radiochemical separation techniques, as compared with pure instrumental proton activation analysis. (RB)

  12. Low-background neutron activation analysis. A powerful tool for atmospheric mineral dust analysis in ice cores

    International Nuclear Information System (INIS)

    The application of instrumental neutron activation analysis (INAA) for multi-elemental analysis of samples of extremely reduced mass such as dust samples extracted from ice cores requires specific efforts towards the development of a 'low level counting' analytical technique. An analytical protocol specifically designed for this kind of samples, based on low-background INAA (LBNAA) is here presented. A first application of the method was successfully performed on samples from the new alpine firn core NextData-LYS12. Sub-ng detection limits were reached for many elements. According to this point the technique is also potentially suitable to be applied to polar ice core samples. (author)

  13. STUDY ON THE ATTACK CONTROL ACTION OF ELECTRO-ACUPUNCTURE ON T(A)IY(A)NG(太阳 EX-HN5)FOR MIGRAINE DUE TO HYPERACTIVITY OF LIVER YANG:RANDOMIED CONTROLED TRIAL%电针太阳穴治疗偏头痛肝阳上亢证的控制发作效应研究

    Institute of Scientific and Technical Information of China (English)

    周建伟; 李季; 李宁; 张凡; 胡玲香; 赵菁菁; 张颜; 王成伟

    2008-01-01

    Objective To discuss the difference of electro-acupuncture and drug in controlling the attack of migraine due to hyperactivity of liver yang.Methods Three-centered random control method was used,and 300 qualified cases were randomly divided into treatment group(146 cases according to the design)and control group(140 cases),which were respectively treated with eIectro-acupuncture and drugs(Compound Aminopyrine Phenacetin Tablets,Ergotamine Caffeine Tablets,Diazepam Tablets),and observed the overall effect and scores of headache,accompanying symptoms,psychological and social adaptability scores,life quality scores,TCM symptoms scores and follow-up results before and after the treatment.Results In treatment group,the successful rate of attack control was 47.3%,the improvement rate was 73.3%,and the total effectiveness was 90.4%,the clinical control rate and improvement were much superior to control group(the clinical controI rate 35.7%,improvement 61.4%,the totaI effectiveness 85.7%),P<0.01,the difference in effect was mainly reflected in patients with moderate severity;the total scores of TCM syndrome after the treatment was obviously significant or very obviously significant(P<0.05,P<0.01),but the headache scores between two groups was not obviously significant(P>0.05),the difference in accompanying symptoms was significant(P<0.01);the long term attack control action in two groups was not satisfactory,the recurrence was similar(P>0.05),the severity of headache in recurrence cases of treatment group was alleviated and superior to control group(P<0.01),the occurrence of headache after the treatment is much less than that before the treatment and the situation after 2 months was superior to controI group(P<0.01).Sleepiness and redness of face in some patients which were seen in control group weren't seen in treatment group.Conclusion Electro-acupuncture on Tàiyáng(太阳EX-HN5)can control the attack of migraine due to hyperactivity of liver yang

  14. Direct Analysis of Amphetamine Stimulants in a Whole Urine Sample by Atmospheric Solids Analysis Probe Tandem Mass Spectrometry

    Science.gov (United States)

    Crevelin, Eduardo J.; Salami, Fernanda H.; Alves, Marcela N. R.; De Martinis, Bruno S.; Crotti, Antônio E. M.; Moraes, Luiz A. B.

    2016-02-01

    Amphetamine-type stimulants (ATS) are among illicit stimulant drugs that are most often used worldwide. A major challenge is to develop a fast and efficient methodology involving minimal sample preparation to analyze ATS in biological fluids. In this study, a urine pool solution containing amphetamine, methamphetamine, ephedrine, sibutramine, and fenfluramine at concentrations ranging from 0.5 pg/mL to 100 ng/mL was prepared and analyzed by atmospheric solids analysis probe tandem mass spectrometry (ASAP-MS/MS) and multiple reaction monitoring (MRM). A urine sample and saliva collected from a volunteer contributor (V1) were also analyzed. The limit of detection of the tested compounds ranged between 0.002 and 0.4 ng/mL in urine samples; the signal-to-noise ratio was 5. These results demonstrated that the ASAP-MS/MS methodology is applicable for the fast detection of ATS in urine samples with great sensitivity and specificity, without the need for cleanup, preconcentration, or chromatographic separation. Thus ASAP-MS/MS could potentially be used in clinical and forensic toxicology applications.

  15. Direct Analysis of Amphetamine Stimulants in a Whole Urine Sample by Atmospheric Solids Analysis Probe Tandem Mass Spectrometry

    Science.gov (United States)

    Crevelin, Eduardo J.; Salami, Fernanda H.; Alves, Marcela N. R.; De Martinis, Bruno S.; Crotti, Antônio E. M.; Moraes, Luiz A. B.

    2016-05-01

    Amphetamine-type stimulants (ATS) are among illicit stimulant drugs that are most often used worldwide. A major challenge is to develop a fast and efficient methodology involving minimal sample preparation to analyze ATS in biological fluids. In this study, a urine pool solution containing amphetamine, methamphetamine, ephedrine, sibutramine, and fenfluramine at concentrations ranging from 0.5 pg/mL to 100 ng/mL was prepared and analyzed by atmospheric solids analysis probe tandem mass spectrometry (ASAP-MS/MS) and multiple reaction monitoring (MRM). A urine sample and saliva collected from a volunteer contributor (V1) were also analyzed. The limit of detection of the tested compounds ranged between 0.002 and 0.4 ng/mL in urine samples; the signal-to-noise ratio was 5. These results demonstrated that the ASAP-MS/MS methodology is applicable for the fast detection of ATS in urine samples with great sensitivity and specificity, without the need for cleanup, preconcentration, or chromatographic separation. Thus ASAP-MS/MS could potentially be used in clinical and forensic toxicology applications.

  16. Antibiotics in the coastal environment of the Hailing Bay region, South China Sea: Spatial distribution, source analysis and ecological risks

    International Nuclear Information System (INIS)

    Highlights: • Thirty-eight antibiotics were systematically investigated in marine environment. • The distribution of antibiotics was significantly correlated with COD and NO3–N. • Untreated domestic sewage was the primary source of antibiotics. • Fluoroquinolones showed a strong sorption capacity onto sediments. • Oxytetracycline, norfloxacin and erythromycin–H2O indicated high risks. - Abstract: In this study, the occurrence and spatial distribution of 38 antibiotics in surface water and sediment samples of the Hailing Bay region, South China Sea, were investigated. Twenty-one, 16 and 15 of 38 antibiotics were detected with the concentrations ranging from <0.08 (clarithromycin) to 15,163 ng/L (oxytetracycline), 2.12 (methacycline) to 1318 ng/L (erythromycin–H2O), <1.95 (ciprofloxacin) to 184 ng/g (chlortetracycline) in the seawater, discharged effluent and sediment samples, respectively. The concentrations of antibiotics in the water phase were correlated positively with chemical oxygen demand and nitrate. The source analysis indicated that untreated domestic sewage was the primary source of antibiotics in the study region. Fluoroquinolones showed strong sorption capacity onto sediments due to their high pseudo-partitioning coefficients. Risk assessment indicated that oxytetracycline, norfloxacin and erythromycin–H2O posed high risks to aquatic organisms

  17. Liquid Chromatography-Tandem Mass Spectrometry Analysis of Perfluorooctane Sulfonate and Perfluorooctanoic Acid in Fish Fillet Samples

    Directory of Open Access Journals (Sweden)

    Viviana Paiano

    2012-01-01

    Full Text Available Perfluorooctane sulfonate (PFOS and perfluorooctanoic (PFOA acid are persistent contaminants which can be found in environmental and biological samples. A new and fast analytical method is described here for the analysis of these compounds in the edible part of fish samples. The method uses a simple liquid extraction by sonication, followed by a direct determination using liquid chromatography-tandem mass spectrometry (LC-MS/MS. The linearity of the instrumental response was good, with average regression coefficients of 0.9971 and 0.9979 for PFOS and PFOA, respectively, and the coefficients of variation (CV of the method ranged from 8% to 20%. Limits of detection (LOD were 0.04 ng/g for both the analytes and recoveries were 90% for PFOS and 76% for PFOA. The method was applied to samples of homogenized fillets of wild and farmed fish from the Mediterranean Sea. Most of the samples showed little or no contamination by perfluorooctane sulfonate and perfluorooctanoic acid, and the highest concentrations detected among the fish species analyzed were, respectively, 5.96 ng/g and 1.89 ng/g. The developed analytical methodology can be used as a tool to monitor and to assess human exposure to perfluorinated compounds through sea food consumption.

  18. Extraction of aflatoxins from liquid foodstuff samples with polydopamine-coated superparamagnetic nanoparticles for HPLC-MS/MS analysis.

    Science.gov (United States)

    McCullum, Cassandra; Tchounwou, Paul; Ding, Li-Sheng; Liao, Xun; Liu, Yi-Ming

    2014-05-14

    A facile magnetic solid phase extraction (MSPE) of aflatoxins (AFs) from liquid samples was developed using polydopamine-coated magnetic nanoparticles (PD-MNPs) as the adsorbent. PD-MNPs were prepared from amine-terminated MNPs and dopamine via an in situ oxidative self-polymerization approach. Under the selected MSPE conditions, extraction yields ranging from 59.3% for AF G2 to 89.0% for AF B1 were obtained with good repeatability. Coupled with HPLC-MS/MS quantification, the MSPE procedure serves not only for sample cleanup but also for AFs enrichment that is highly desired for trace analysis. The proposed MSPE-HPLC-MS/MS method had a linear calibration curve in the concentration range from 0.00600 to 3.00 ng/mL aflatoxin and limits of detection of 0.0012 ng/mL for AF B1, AF B2, and AF G1, and 0.0031 ng/mL for AF G2. PMID:24766417

  19. A novel vacuum spectrometer for total reflection x-ray fluorescence analysis with two exchangeable low power x-ray sources for the analysis of low, medium, and high Z elements in sequence

    Energy Technology Data Exchange (ETDEWEB)

    Wobrauschek, P., E-mail: wobi@ati.ac.at; Prost, J.; Ingerle, D.; Kregsamer, P.; Streli, C. [Atominstitut, TU Wien, Stadionallee 2, 1020 Vienna (Austria); Misra, N. L. [Fuel Chemistry Division, Bhabha Atomic Research Centre (BARC), Mumbai 400085 (India)

    2015-08-15

    The extension of the detectable elemental range with Total Reflection X-ray Fluorescence (TXRF) analysis is a challenging task. In this paper, it is demonstrated how a TXRF spectrometer is modified to analyze elements from carbon to uranium. Based on the existing design of a vacuum TXRF spectrometer with a 12 specimen sample changer, the following components were renewed: the silicon drift detector with 20 mm{sup 2} active area and having a special ultra-thin polymer window allowing the detection of elements from carbon upwards. Two exchangeable X-ray sources guarantee the efficient excitation of both low and high Z elements. These X-ray sources were two light-weighted easily mountable 35 W air-cooled low-power tubes with Cr and Rh anodes, respectively. The air cooled tubes and the Peltier-cooled detector allowed to construct a transportable tabletop spectrometer with compact dimensions, as neither liquid nitrogen cooling for the detector nor a water cooling circuit and a bulky high voltage generator for the X-ray tubes are required. Due to the excellent background conditions as a result of the TXRF geometry, detection limits of 150 ng for C, 12 ng for F, and 3.3 ng for Na have been obtained using Cr excitation in vacuum. For Rh excitation, the detection limits of 90 pg could be achieved for Sr. Taking 10 to 20 μl of sample volume, extrapolated detection limits in the ng/g (ppb) range are resulting in terms of concentration.

  20. A novel vacuum spectrometer for total reflection x-ray fluorescence analysis with two exchangeable low power x-ray sources for the analysis of low, medium, and high Z elements in sequence

    International Nuclear Information System (INIS)

    The extension of the detectable elemental range with Total Reflection X-ray Fluorescence (TXRF) analysis is a challenging task. In this paper, it is demonstrated how a TXRF spectrometer is modified to analyze elements from carbon to uranium. Based on the existing design of a vacuum TXRF spectrometer with a 12 specimen sample changer, the following components were renewed: the silicon drift detector with 20 mm2 active area and having a special ultra-thin polymer window allowing the detection of elements from carbon upwards. Two exchangeable X-ray sources guarantee the efficient excitation of both low and high Z elements. These X-ray sources were two light-weighted easily mountable 35 W air-cooled low-power tubes with Cr and Rh anodes, respectively. The air cooled tubes and the Peltier-cooled detector allowed to construct a transportable tabletop spectrometer with compact dimensions, as neither liquid nitrogen cooling for the detector nor a water cooling circuit and a bulky high voltage generator for the X-ray tubes are required. Due to the excellent background conditions as a result of the TXRF geometry, detection limits of 150 ng for C, 12 ng for F, and 3.3 ng for Na have been obtained using Cr excitation in vacuum. For Rh excitation, the detection limits of 90 pg could be achieved for Sr. Taking 10 to 20 μl of sample volume, extrapolated detection limits in the ng/g (ppb) range are resulting in terms of concentration

  1. Analysis of aflatoxin b1 in Iranian foods using HPLC and a monolithic column and estimation of its dietary intake.

    Science.gov (United States)

    Yazdanpanah, Hassan; Zarghi, Afshin; Shafaati, Ali Reza; Foroutan, Seyed Mohsen; Aboul-Fathi, Farshid; Khoddam, Arash; Nazari, Firoozeh; Shaki, Fatemeh

    2013-01-01

    A high performance liquid chromatographic method was developed for determination of aflatoxin B1 (AFB1) in foods using a monolithic column with sample clean up on an immunoaffinity column. The method was validated for analysis of AFB1 in rice, bread, puffed corn snack, wheat flour and peanut samples. The average recoveries for AFB1 in different foods ranged from 94.4 to 102.5% with the coefficient of variation lower than 10% for all foods. Limit of detection was 0.01 ng/g. A survey of AFB1 was performed on 90 samples collected from Tehran retail market in June 2005. The results showed that none of the bread and wheat flour samples were contaminated with AFB1. The mean AFB1 levels in rice, puffed corn snack and peanut samples were 4.17, 0.11, and 1.97 ng/g, respectively. The level of contamination of 3 samples (one rice sample and two peanuts samples) to AFB1 was found to be higher than 5 ng/g. Although all food samples had mean concentration of AFB1 below the maximum tolerated level in Iran, the mean intake of AFB1 from rice was estimated 3.49 times higher than the guidance value of 1 ng AFB1/Kg body weight/day. Therefore, it is strongly recommended to monitor AFB1 in foods, especially in rice, in Iran. This is the first study on exposure assessment of Iranian population to AFB1. PMID:24250676

  2. Stable Isotope Dilution Analysis of Gibberellin Residues in Tomato Paste by Liquid Chromatography-Tandem Mass Spectrometry

    Institute of Scientific and Technical Information of China (English)

    SUN Li; ZHAO Yan-sheng; NIE Xue-mei; LING Yun; CHU Xiao-gang; SHANG De-jun; DONG Ying

    2012-01-01

    An accurate and sensitive method for the simultaneous determination of gibberellic acid(GA3),gibberellin A4(GA4) and gibberellin A7(GA7) residues in tomato paste was developed by coupling solid phase extraction to high performance liquid chromatography-tandem mass spectrometry(LC-MS/MS) with electrospray ionization based stable isotope dilution analysis(SIDA).The isotope labeled internal standard can compensate for the losses during the extraction and cleanup steps and for discrimination due to ion suppression.After extraction from methanol,hydrophile lipophilic balance(HLB) solid phase extraction(SPE) column was tested for the capacity of the cleanup of the tomato paste in compared with C18 SPE column which is the common way to the detection of GAs,and the former gained better result.Spiked experiments were performed in the non-contaminated tomato pastes and the recoveries of GA3,GA4 and GA7 were 42.6%-75.0% in external standard method(ESM) and 91.1%-103.8% in internal standard method(ISM) respectively.The validities of this method were investigated and good analytical performance for the three GAs was obtained,including low limits of method detection(2 ng/g for GA3 and GA4,0.3 ng/g for GA7),excellent linear dynamic ranges(5-500 ng/g for GA3 and GA4,1-100 ng/g for GAy) and good relative standard deviation ranges(4.8%-9.4% for the intra-day test and 3.5%-11.9% for the inter-day test).

  3. [Risk assessment and analysis of polycyclic aromatic hydrocarbons in the surface sediments of Xinglin Bay Suburb Rivers of Xiamen].

    Science.gov (United States)

    Cheng, Qi-ming; Huang, Qing; Liao, Zhen-ni; Su, Li; Liu, Xing-qiang; Tang, Jian-feng

    2015-01-01

    The concentrations of 16 polycyclic aromatic hydrocarbons (PAHs), declared as carcinogens by USEPA, were measured in the sediment samples (n = 19) collected along Xinglin Bay rivers/canals present in Xiamen. PAHs were extracted using accelerated solvent extraction system and analyzed using gas chromatography-mass spectrometry (GC-MS). The possible sources of PAHs and their health risk were investigated. Among selected PAHs, 13 PAHs were detected in the surface sediments. Total concentrations of PAHs in the surface sediments ranged from 413.00 to 2748.81 ng x g(-1), with a mean value of 949.56 ng x g(-1). The mean concentration of highly carcinogenic compounds such as benzo [k] fluoranthene (BkF) and benzo [g, h, i] perylene (BghiP) were 69.15 ng x g(-1) and 49.86 ng x g(-1), respectively and counted for 73.68% out of the total samples. The dominant PAH compounds were 2, 3 and 4 rings and counted for 61.03% and 23.53% , respectively; while 5-6 rings containing PAHs were accounted for 15.82%. According to the results, 68.42% of samples were moderately contaminated, while 31.58% were highly contaminated with PAHs. Based on the ratios of Ant/(Ant + Phe) and Fla/(Fla + Pyr), it cleared that PAHs in surface sediments were mainly derived from fossil fuel and combustion products. Principal component analysis results and sediment quality benchmarks (mSQG-Q) were used for risk assessment of these PAH contaminated sediments. The risk SQG-Q of 16 PAHs were less than 0.50. Comprehensive content, composition and SQG-Q showed that a certain degree of ecological risks of PAH pollution existed in the surface sediments, particularly in the sites close to Xinglin Industrial Zone (2, 3, 5 and 9) and Gangtou(13), which need further research work and proper attentions. PMID:25898662

  4. Different headspace solid phase microextraction--gas chromatography/mass spectrometry approaches to haloanisoles analysis in wine.

    Science.gov (United States)

    Jeleń, Henryk H; Dziadas, Mariusz; Majcher, Małgorzata

    2013-10-25

    Three approaches in determination of six haloanisoles (2,4,6-trichloroanisole, 2,3,4-trichloroanisole, 2,3,6-trichloroanisole, tetrachloroanisole, pentachloroanisole and 2,4,6-tribromoanisole) in wine were compared. Comprehensive gas chromatography - time of flight mass spectrometry (GC×GC-ToF-MS) was described for the first time for this application and compared to gas chromatography-tandem mass spectrometry (GC-MS/MS) using triple quadrupole instrument. These techniques were compared with "standard" analytical approach using GC-MS(SIM). SPME method was developed and used for all separation methods (DVB/PDMS fiber, 70 °C, 30%NaCl, 20 min extraction). Extraction dependence on matrix was discussed using model wines with different ethanol contents (8%, 12%, and 18%) as well as water and different wines (dry white, dry red and sweet liqueur), with the lowest sensitivities obtained for highest ethanol contents in model wine and for liqueur wine. Limits of detection for GC×GC-ToF-MS method were 0.09-2.92 ng/L depending on the examined compound and matrix (compared to 0.1-13.3 ng/L obtained using GC/MS(SIM)). For GC-MS/MS method lower detection limits were achieved than for the GC×GC method (0.01-0.1 ng/L), however comprehensive gas chromatography-mass spectrometry provides full spectral information on analyzed compounds. Both methods had limits of detection far below odor thresholds of haloanisoles in wine, good linearity up to 2000 ng/L tested and good precision, what makes them suitable for analysis of these compounds in low ppt levels. PMID:23932370

  5. Advanced Inductively Coupled Plasma-Mass Spectrometry Analysis of Rare Earth Elements: Environmental Applications

    OpenAIRE

    Zhu, W

    1999-01-01

    Rare Earth Elements (REEs) are a group of 16 related elements with low concentrations in air particulate matter, soil and water. An analytical technique was devised capable of measuring simultaneously all 16 REEs at very low levels and at great speed in environmental matrices. The ICP-MS in combination with the PLSR model and ion exchange preconcentration offers a fast multi-element analysis technique with high precision and accuracy and detection limits of below 1 ng.r\\ REEs were analysed in...

  6. Advanced inductively coupled plasma mass spectrometry analysis of rare earth elements: environmental applications

    OpenAIRE

    Zhu, W.

    1999-01-01

    Rare Earth Elements (REEs) are a group of 16 related elements with low concentrations in air particulate matter, soil and water. An analytical technique was devised capable of measuring simultaneously all 16 REEs at very low levels and at great speed in environmental matrices. The ICP-MS in combination with the PLSR model and ion exchange preconcentration offers a fast multi-element analysis technique with high precision and accuracy and detection limits of below 1 ng.r\\ REEs were analysed in...

  7. Validation of an analytical methodology for the quantitative analysis of petroleum hydrocarbons in marine sediment samples

    OpenAIRE

    Eloy Yordad Companioni Damas; Miriam Odette Cora Medina; Ana Catalina Núñez Clemente; Miguel Ángel Díaz Díaz; Luis González Bravo; Rolando Marbot Ramada; Rodny Montes de Oca Porto

    2009-01-01

    This work describes a validation of an analytical procedure for the analysis of petroleum hydrocarbons in marine sediment samples. The proposed protocol is able to measure n-alkanes and polycyclic aromatic hydrocarbons (PAH) in samples at concentrations as low as 30 ng/g, with a precision better than 15% for most of analytes. The extraction efficiency of fortified sediments varied from 65.1 to 105.6% and 59.7 to 97.8%, for n-alkanes and PAH in the ranges: C16 - C32 and fluoranthene - benzo(a)...

  8. The Computer Aided Integrated Design and Analysis of Oil Tank in Vehicle

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    This paper creates 3D solid model and assembly of U RJ 92-6 oil tank and analyses its strength by integrated CAD/CAE/CAM software I-D EAS. Through integrated simulation in computer, design efficiency and quality of oil tank is greatly improved. Adopting integrated CAD/CAE/CAM software to carry out integrated research to equ ipment and products, we will be able to take overall analysis in aspects of 3-D solid modeling, pre-assembly and strength, etc., to realize non-paper designi ng and parallel designing...

  9. Analysis of Mercury-Rich plants and mine tailings using the Hydride-Generation AAS method

    OpenAIRE

    Fábio Netto Moreno; Christopher Anderson; Robert Stewart; Brett Robinson

    2009-01-01

    A hydride-generation atomic absorption spectroscopy (AAS) method was developed for the analysis of total Hg in liquid matrices of mercury-rich plants and mine tailings samples. The detection limit for this method was as low as 11.4 ng/mL. The reproducibility of the mercury signals (in terms of relative standard deviation) was 4.6%. Accuracy of the method was verified by analyses of deionised water samples spiked with HgCl2 and HgNO3. Recovery values for total mercury ranged from 88.5 to 94.3%...

  10. Nondestructive determination of arsenic in urine by epithermal neutron activation analysis and Compton suppression.

    Science.gov (United States)

    Landsberger, S; Swift, G; Neuhoff, J

    1990-01-01

    Epithermal neutron activation analysis, in conjunction with Compton suppression, has been employed to determine arsenic levels in artificially doped urine samples. Typical detection limits were of the order of 10 ng/g. Replicate determinations gave precision values between 2 and 12%, whereas accuracy measurements were between +/- 1 and +/- 20%. Biological and geological reference materials from the National Institute of Standards and Technology (NIST) were also analyzed for arsenic content. Typically, the precision achieved again was between 2 and 12%, whereas the accuracy measurements were in excellent agreement with the certified values. PMID:1704729

  11. Analysis of PAHs Associated with Particulate Matter PM2.5 in Two Places at the City of Cuernavaca, Morelos, México

    Directory of Open Access Journals (Sweden)

    Hugo Saldarriaga-Noreña

    2015-08-01

    Full Text Available This study was carried out between January and February 2013, at two sites in the city of Cuernavaca, México, using low-volume equipment. Fifteen Polycyclic aromatic hydrocarbons (PAHs, were identified by gas chromatography coupled with mass spectrometry. The total average concentration observed for PAHs was 24.0 ng·m−3, with the high molecular weight compounds being the most abundant. The estimated equivalent concentration for Benzo (a P (BaPE was 4.05 ng·m−3. Diagnostic ratios together with the principal components analysis (PCA allowed for establishing coal burning and vehicle emissions as being the main sources of these compounds in the area. The PAHs used to calculate this index account for 51% of the 15 PAHs identified, which probably involves a risk to the exposed population.

  12. Simultaneous analysis of multiple classes of antimicrobials in environmental water samples using SPE coupled with UHPLC-ESI-MS/MS and isotope dilution.

    Science.gov (United States)

    Tran, Ngoc Han; Chen, Hongjie; Do, Thanh Van; Reinhard, Martin; Ngo, Huu Hao; He, Yiliang; Gin, Karina Yew-Hoong

    2016-10-01

    A robust and sensitive analytical method was developed for the simultaneous analysis of 21 target antimicrobials in different environmental water samples. Both single SPE and tandem SPE cartridge systems were investigated to simultaneously extract multiple classes of antimicrobials. Experimental results showed that good extraction efficiencies (84.5-105.6%) were observed for the vast majority of the target analytes when extraction was performed using the tandem SPE cartridge (SB+HR-X) system under an extraction pH of 3.0. HPLC-MS/MS parameters were optimized for simultaneous analysis of all the target analytes in a single injection. Quantification of target antimicrobials in water samples was accomplished using 15 isotopically labeled internal standards (ILISs), which allowed the efficient compensation of the losses of target analytes during sample preparation and correction of matrix effects during UHPLC-MS/MS as well as instrument fluctuations in MS/MS signal intensity. Method quantification limit (MQL) for most target analytes based on SPE was below 5ng/L for surface waters, 10ng/L for treated wastewater effluents, and 15ng/L for raw wastewater. The method was successfully applied to detect and quantify the occurrence of the target analytes in raw influent, treated effluent and surface water samples. PMID:27474294

  13. Multiresidue analysis of 88 polar organic micropollutants in ground, surface and wastewater using online mixed-bed multilayer solid-phase extraction coupled to high performance liquid chromatography-tandem mass spectrometry.

    Science.gov (United States)

    Huntscha, Sebastian; Singer, Heinz P; McArdell, Christa S; Frank, Carolin E; Hollender, Juliane

    2012-12-14

    An automated multiresidue method consisting of an online solid-phase extraction step coupled to a high performance liquid chromatography-tandem mass spectrometer (online-SPE-HPLC-MS/MS method) was developed for the determination of 88 polar organic micropollutants with a broad range of physicochemical properties (logD(OW) (pH 7): -4.2 to 4.2). Based on theoretical considerations, a single mixed-bed multilayer cartridge containing four different extraction materials was composed for the automated enrichment of water samples. This allowed the simultaneous analysis of pesticides, biocides, pharmaceuticals, corrosion inhibitors, many of their transformation products, and the artificial sweetener sucralose in three matrices groundwater, surface water, and wastewater. Limits of quantification (LOQs) were in the environmentally relevant concentration range of 0.1-87 ng/L for groundwater and surface water, and 1.5-206 ng/L for wastewater. The majority of the compounds could be quantified below 10 ng/L in groundwater (82%) and surface water (80%) and below 100 ng/L in wastewater (80%). Relative recoveries were largely between 80 and 120%. Intraday and inter-day precision, expressed as relative standard deviation, were generally better than 10% and 20%, respectively. 50 isotope labeled internal standards were used for quantification and accordingly, relative recoveries as well as intraday and inter-day precision were better for compounds with corresponding internal standard. The applicability of this method was shown during a sampling campaign at a riverbank filtration site for drinking water production with travel times of up to 5 days. 36 substances of all compound classes investigated could be found in concentrations between 0.1 and 600 ng/L. The results revealed the persistence of carbamazepine and sucralose in the groundwater aquifer as well as degradation of the metamizole metabolite 4-acetamidoantipyrine. PMID:23137864

  14. Ang Pagsasakatutubo mula sa Loob/Kultural na Pagpapatibay ng mga Salitang Pandamdaming Tumutukoy sa “Sayá”: Isang Semantikal na Elaborasyon ng Wikang Filipino sa Larangan ng Sikolohiya

    Directory of Open Access Journals (Sweden)

    Jayson D. Petras

    2013-12-01

    Full Text Available The Philippines has often been recognized as one of the most emotional countries in the world. Despite this, there is a scarcity of research pertaining to emotions in the context of Filipinos’ own language and culture; instead, the convenient practice of explaining phenomena based on studies published abroad continues. This is the reason why even local scholarship remains ethnocentric, particularly Anglocentric, in nature. This paper answers the need to contextualize studies about emotions by adopting cultural revalidation or indigenization from within that was developed by the Father of Filipino Psychology, Virgilio G. Enriquez, as an approach to the semantic elaboration of Filipino words pertaining to “happiness.” In analyzing the scope and depth of such words, as well as their similarities and differences, it is essential to consider the universal aspect of understanding emotion through the Natural Semantic Metalanguage (NSM developed by Anna Wierzbicka and her colleagues and the particular aspect embedded in the loob-labas (internal-external dimensions and the Filipino culture-specif ic use of language. Through this, highlighting the uniqueness of the words alíw, galák, ligáya, lugód, luwalhatì, sayá, siyá, tuwâ, and wíli becomes possible. In the end, the researcher poses the challenge of continuous examination of the language of emotion toward understanding Filipino personality.

  15. EFFECTS OF TEACHİNG METACOGNİTİON STRATEGİES TO LİSTENİNG COMPREHENSİON SKİLLS AND ATTİTUDE TOWARD LİSTENİNG

    OpenAIRE

    KATRANCI, Mehmet; YANGIN, BANU

    2013-01-01

    The purpose of this research is determining the effect of teaching metacognition strategies to the fifth grade students according to their listening comprehension skills and students’ attitudes toward listening. The study group of the research consists of 65 fifth grade students at two elementary schools in the province of Kırıkkale. In the study, mixed method which both quantitative and qualitative research methods are used together for collecting, analysing and interpreting data. During the...

  16. Pilipino Language Arts Level One--Identity. Reader: "At School" [and] Teacher's Handbook [and] Student Workbook=Sining ng Wika Unang Antas--Pagkakakilanlan. Reader: "Sa Paaralan" [and] Hanbuk ng Guro [and] Gawaing-aklat ng Mag-aaral.

    Science.gov (United States)

    Gungab, Voltaire

    As part of the Asian American Bilingual Center (AABC) Pilipino Language Arts Program, a curriculum suitable for students in grades 1 and 2 is presented. The program is geared both to the immigrant Filipino child and to the American-born Filipino child who learned to speak Pilipino at home, and includes kits that emphasize the AABC's major…

  17. Pilipino Language Arts Level One--Needs. Reader: "Our Surroundings" [and] Teacher's Handbook [and] Student Workbook=Sining ng Wika Unang Antas--Pangangailangan. Reader: "Ang Paligid Natin" [and] Hanbuk ng Guro [and] Gawaing-aklat ng Mag-aaral.

    Science.gov (United States)

    Gungab, Voltaire

    As part of the Asian American Bilingual Center (AABC) Pilipino Language Arts Program, a curriculum suitable for students in grades 1 and 2 is presented. The program is geared both to the immigrant Filipino child and to the American-born Filipino child who learned to speak Pilipino at home, and includes kits that emphasize the AABC's major…

  18. Multiresidue Analysis of Pesticides in Straw Roughage by Liquid Chromatography-Tandem Mass Spectrometry.

    Science.gov (United States)

    Zhang, Zihao; Feng, Mengyuan; Zhu, Kechen; Han, Lijun; Sapozhnikova, Yelena; Lehotay, Steven J

    2016-08-10

    A multiresidue analytical method using a modification of the "quick, easy, cheap, effective, rugged, and safe" (QuEChERS) sample preparation approach combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis was established and validated for the rapid determination of 69 pesticides at different levels (1-100 ng/g) in wheat and rice straws. In the quantitative analysis, the recoveries ranged from 70 to 120%, and consistent RSDs ≤ 20% were achieved for most of the target analytes (53 pesticides in wheat straw and 58 in rice straw). Almost all of the analytes achieved good linearity with R(2) > 0.98, and the limit of validation levels (LVLs) for diverse pesticides ranged from 1 to 10 ng/g. Different extraction and cleanup conditions were evaluated in both types of straw, leading to different options. The use of 0.1% formic acid or not in extraction with acetonitrile yielded similar final outcomes, but led to the use of a different sorbent in dispersive solid-phase extraction. Both options are efficient and useful for the multiresidue analysis of targeted pesticides in wheat and rice straw samples. PMID:26881844

  19. Online eluent-switching technique coupled anion-exchange liquid chromatography–ion trap tandem mass spectrometry for analysis of non-steroidal anti-inflammatory drugs in pig serum.

    Science.gov (United States)

    Chang, Kai Chun; Lin, Jyh Shiun; Cheng, Cheanyeh

    2015-11-27

    A novel method for online extraction, pH-gradient separation, and analysis of nine non-steroidal anti-inflammatory drugs (NSAIDs) was developed by coupling online eluent-switching technique to single anion-exchange chromatographic column/ion trap mass spectrometer (MS) and used for monitoring NSAIDs residues in pig serum. A neutral eluent and a pH-gradient eluent were used for extraction and separation of NSAIDs, respectively. Each of nine NSAIDs has an MS precursor ion of either [M−H]− or [M−Na]−. The extracted ion chromatogram for a specific product ion of each NSAID was used for its quantitative analysis. The dynamic linear ranges of calibration curves were all 0–200 ng mL−1 (R2 > 0.9950). The analysis accuracies estimated by spiking standard concentrations at 20, 100, and 200 ng mL−1 were 80.5–99.9%. The corresponding intra-day and inter-day precisions (RSD%) were 2.5–14.5% and 2.9–15.2%, respectively. The limit of detection/limit of quantitation of NSAIDs were 1.3/4.3, 0.5/1.6, 0.2/0.5, 2.5/8.2, 1.5/4.9, 0.6/2.1, 0.6/2.0, 0.5/1.7, and 0.6/2.1 ng mL−1 for carprofen, diclofenac, flunixin, ibuprofen, ketoprofen, meclofenamic acid sodium, mefenamic acid, niflumic acid, and tolfenamic acid, respectively. After 1 h injection of a dose containing 2 mg kg−1 weight pig of flunixin and tolfenamic acid to the pigs, a residue amount of 3480 ± 36 ng mL−1 and 431 ± 13 ng mL−1, respectively, was reached for the incurred pig serum specimens and both residues were reduced to about 20 ng mL−1 at the time of 24 h. PMID:26601710

  20. Establishment of Exposure to Organophosphorus Warfare Agents by Means of SPME-GSMS Analysis of Bodily Fluids

    International Nuclear Information System (INIS)

    Reliable chemical analytical procedures for revealing an exposure to toxic chemicals, identifying the active substance, and assessing the degree of exposure are necessary as a component of medical and forensic activities in cases of the possible use of highly toxic chemicals in war conflicts and terrorism acts, as well as emergency situations in chemical industry, specifically at chemical weapons storage and destruction facilities. According to Chemical Weapons Convention, Part XI, Appendix 4, e-17, 'samples of importance in the investigation of alleged use include biomedical samples from human or animal sources (blood, urine, excreta, tissue etc.)'. Urinary metabolites, O-alkyl esters of methylphosphic acid, offer one of the simplest means of confirming an exposure to organophosphorus warfare agents (OPWA). Urine, unlike blood or tissues, does not require invasive collection demanding in terms of sterility. Excretion with urine is the major route of elimination of OPWA from an organism. According to published data, 90% of OPWA metabolites are excreted within 48-72 h after intoxication. We developed an SPME-GCMS procedure for the determination of O-alkyl esters methylphosphonic acid in urine, with the following detection limits,: isopropyl and isobutyl esters 5 ng/ml and pinacolyl ester 1 ng/ml. The procedure involves derivatization of the target compounds directly on the microfiber. The total analysis time is 1-1.5 h. In animal experiments in vivo we could establish the exposure to OPWA at a half-LD50 level within no less than 48 h after intoxication. In principle, OPWA metabolites could be detected in urine within two weeks after intoxication but at higher doses. Retrospective analysis of urinary metabolites in cases of the exposure to low doses of OPWA requires lower detection limits (0.1-1 ng/ml). Optimal objects for the retrospective analysis of OPWA in an organism are long-lived blood protein adducts. We developed a procedure for revealing an exposure to

  1. Hair analysis to monitor abuse of analgesic combinations containing butalbital and propyphenazone.

    Science.gov (United States)

    Ferrari, Anna; Tiraferri, Ilaria; Palazzoli, Federica; Verri, Patrizia; Vandelli, Daniele; Marchesi, Filippo; Ciccarese, Michela; Licata, Manuela

    2015-11-10

    Butalbital, a barbiturate, is present in analgesic combinations used by headache sufferers. Overuse/abuse of these combinations may cause dependence, chronic migraine, and medication-overuse headache (MOH). MOH is difficult to manage: it improves interrupting analgesic overuse, but requires monitoring, because relapses are frequent. A gas chromatography-mass spectrometry (GC-MS) method for hair analysis has been developed and validated to document abuse of an analgesic combination containing butalbital and propyphenazone by a patient with MOH. For over ten years the patient managed her headache using eight suppositories/day of an analgesic combination containing butalbital 150mg, caffeine 75mg, and propyphenazone 375mg per suppository. An outpatient detoxification treatment was carried out. After three weeks, the patient reduced the consumption to one suppository/day. At the first control visit, after three months from the beginning of detoxification, the patient increased the use of the combination to four suppositories/day and at the second control visit, after seven months from the beginning of detoxification, she was back to eight suppositories/day. At the two control visits, a hair sample was taken for determination of butalbital and propyphenazone. Moreover blood and urine samples for determination of butalbital were drawn at the beginning of detoxification treatment and at the two control visits. With the segmental analysis of two hair samples the medication history of ten months could be estimated. In the first hair sample, collected at the first control visit, in the distal segment, butalbital and propyphenazone concentrations were, respectively, 17.5ng/mg and 56.0ng/mg, confirming the prolonged abuse; in the proximal segment, concurrently with the detoxification treatment, butalbital and propyphenazone concentrations had reduced respectively to 5.45ng/mg and 11.1ng/mg. The second hair sample, collected at the second control visit, proved the fair course

  2. Tramadol chronic abuse: an evidence from hair analysis by LC tandem MS.

    Science.gov (United States)

    Verri, Patrizia; Rustichelli, Cecilia; Palazzoli, Federica; Vandelli, Daniele; Marchesi, Filippo; Ferrari, Anna; Licata, Manuela

    2015-01-01

    Hair analysis, as complementary matrix, has expanded across the spectrum of toxicological investigations for misuse drug monitoring. Hair has become an important matrix for drug analysis, owing to the possibility to detect target analytes for long time periods, depending on hair length. A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method has been developed for the quantitation of tramadol, a widely used centrally acting analgesic, and its main metabolites in hair (ODMT, NDMT, NOT). Hair samples were decontaminated and incubated overnight in diluted hydrochloric acid; the extracts were purified by mixed-mode solid phase cartridges and analyzed by LC-MS/MS in positive ionization mode monitoring two transitions per analyte. The procedure was fully validated in terms of linearity, limit of detection and lower limit of quantitation (LLOQ), accuracy, precision, recovery, matrix effect and selectivity. The linear regression analysis was calibrated by deuterated internal standards; for all analytes, responses were linear over the range 0.04-40.00 ng/mg hair, with R(2) values of at least 0.995. The method offered satisfactory precision (RSD 90%) values. The found LLOQ values for tramadol and metabolites were in the range 0.010-0.030 ng/mg hair. The proposed procedure was successfully applied to quantify tramadol and metabolites in real hair samples submitted to our laboratory: three cases of tramadol assumption within the therapeutic dosage (3 × 2 segments) and one case of tramadol abuse in a binge pattern (8 segments). The ranges found for TRAM, ODMT, NDMT and NOT were markedly higher in the abuse case (63.42-107.30, 3.76-6.26, 24.88-45.66, 0.22-1.18 ng/mg hair, respectively) compared to the other case reports (3.29-20.12, 0.28-1.87, 0.45-4.32, 0.07-0.80 ng/mg, respectively); also the values of NMDT/ODMT ratio differed significantly. According to the obtained data, we hypothesized that the binge pattern may influence the metabolites' to parent drug

  3. Determination of propoxur in environmental samples by automated solid-phase extraction followed by flow-injection analysis with tris(2,2'-bipyridyl)ruthenium(II) chemiluminescence detection

    International Nuclear Information System (INIS)

    A sensitive method for the analysis of propoxur in environmental samples has been developed. It involves an automated solid-phase extraction (SPE) procedure using a Gilson Aspec XLi and flow-injection analysis (FI) with chemiluminescence (CL) detection. The FI-CL system relies on the photolysis of propoxur by irradiation using a low-pressure mercury lamp (main spectral line 254 nm). The resultant methylamine is subsequently detected by CL using tris(2,2'-bipyridyl)ruthenium(III), which is on-line generated by photo-oxidation of the ruthenium(II) complex in the presence of peroxydisulfate. The linear concentration range of application was 0.05-5 μg mL-1 of propoxur, with a detection limit of 5 ng mL-1. The repeatability was 0.82% expressed as relative standard deviation (n = 10) and the reproducibility, studied on 5 consecutive days, was 2.1%. The sample throughput was 160 injection per hour. Propoxur residues below ng mL-1 levels could be determined in environmental water samples when an SPE preconcentration device was coupled on-line with the FI system. This SPE-FI-CL arrangement provides a detection limit as low as 5 ng L-1 using only 500 mL of sample. In the analysis of fruits and vegetables, the detection limit was about 10 μg kg-1

  4. Cobalt internal standard for Ni to assist the simultaneous determination of Mo and Ni in plant materials by high-resolution continuum source graphite furnace atomic absorption spectrometry employing direct solid sample analysis.

    Science.gov (United States)

    de Babos, Diego Victor; Bechlin, Marcos André; Barros, Ariane Isis; Ferreira, Edilene Cristina; Gomes Neto, José Anchieta; de Oliveira, Silvana Ruella

    2016-05-15

    A new method is proposed for the simultaneous determination of Mo and Ni in plant materials by high-resolution continuum source graphite furnace atomic absorption spectrometry (HR-CS GFAAS), employing direct solid sample analysis (DSS) and internal standardization (IS). Cobalt was used as internal standard to minimize matrix effects during Ni determinations, enabling the use of aqueous standards for calibration. Correlation coefficients for the calibration curves were typically better than 0.9937. The performance of the method was checked by analysis of six plant certified reference materials, and the results for Mo and Ni were in agreement with the certified values (95% confidence level, t-test). Analysis was made of different types of plant materials used as renewable sources of energy, including sugarcane leaves, banana tree fiber, soybean straw, coffee pods, orange bagasse, peanut hulls, and sugarcane bagasse. The concentrations found for Mo and Ni ranged from 0.08 to 0.63 ng mg(-1) and from 0.41 to 6.92 ng mg(-1), respectively. Precision (RSD) varied from 2.1% to 11% for Mo and from 3.7% to 10% for Ni. Limits of quantification of 0.055 and 0.074 ng were obtained for Mo and Ni, respectively. PMID:26992542

  5. The Application of Voltammetric Analysis of Δ(9) -THC for the Reduction of False Positive Results in the Analysis of Suspected Marijuana Plant Matter.

    Science.gov (United States)

    Balbino, Marco A; de Oliveira, Laura S; Eleotério, Izabel C; Oiye, Erica N; Ribeiro, Maria F M; McCord, Bruce R; Ipolito, Antonio J; de Oliveira, Marcelo F

    2016-07-01

    The development of methodologies using inexpensive, fast, and reliable instrumention is desirable in illicit drug analysis. The purpose of this study was based on cyclic voltammetry technique to differentiate the electrochemical behavior of ∆(9) -THC, the psychoactive substance in marijuana, and five different extract plants to yield false positive results after analysis protocol for cannabinoids using thin-layer chromatography and Fast Blue B salt. After applying a deposition potential of -0.5 V in a glassy carbon working electrode, the results indicated an anodic peak current at 0.0 V versus Ag/AgCl after addition of ∆(9) -THC solution in the electrochemical cell, and limits of detection and quantification were 1.0 ng mL(-1) and 3.5 ng mL(-1) , respectively. Other interfering plants showed distinct amperometric responses. This methodology was useful to detect ∆(9) -THC even in the presence of the Fast Blue B salt, which avoided false positive results for all the studied extract plants. PMID:27364289

  6. A non-derivatization Analysis of Fatty Acids from C6 to C20 in PM 2.5 Aerosols using GC/FID and GC/MSD

    Science.gov (United States)

    Cheng, Y.; Li, S.

    2002-12-01

    Fatty acids make large contributions to aerosol mass. In particular, palmitic acid (C16) and stearic acid (C18) are dominant in atmosphere and in many sources of aerosols. Their analysis after sampling is usually based on methylation or silanization into methyl esters or silanes for analysis on GC/FID or GC/MSE. In this paper, a non-derivatization analysis method of fatty acids ranged from C6 to C20 in PM2.5 aerosols is discussed. The entire analysis method includes the processes of extraction using Accelerated Solvent Extraction (ASE), cleanup using silica gel column chromatography, concentration by blow down, and GC/FID and GC/MSD analysis/identification with the DB-FFAP capillary column. The calibration curves of the fatty acids on the GC/FID basically go through the origin and have highly linear response (r2 from 0.99862 to 0.99979) for the concentrations ranged from 0.6 to 128 ng/μl. Detection limits of fatty acids on the GC/FID are from 0.2 to 0.6 ng/μl depending on the compounds. The linear response of the calibration curves on the GC/FID is from 0.92 to 0.99. The detect limits on the GC/MSD are around 0.8 ng/μl. The relative standard deviations (RSD) of quantitation on the GC/FID are smaller than on the GC/MSD. Using the GC/FID, the recoveries of fatty acids using the entire method with spiked internal standards (surrogates) are between 80 and 104% with the RSD extracted at 80°C on the ASE the compounds were not stable and later turned into their methyl esters within one month, as well as creating some contamination peaks that rendered the quantitative analysis useless.

  7. Perkembangan Seni Beladiri Wushu Di Kota Medan Tahun (2001-2013) 印尼棉兰人武术传统的发展研究(Yìnní miánlán rén wǔshù chuántǒng de fāzhǎn yánjiū)

    OpenAIRE

    Purba, Dedi A.

    2015-01-01

    The title of this research is “ Perkembangan Seni Beladiri Wushu di Kota Medan ( 2001 – 2013)”. In this research, the writer is trying to describe the Wushu, especially in the development of wushu martial arts . Due the title, there are some concepts written in chapter two, they are development, martial arts and wushu. The theory used in this research is social theory or culture theory, that is used to analysis the development of wushu martial arts . The methodology used on the research to ...

  8. Analysis of metals and phosphorus in biodiesel B100 from different feedstock using a Flow Blurring® multinebulizer in inductively coupled plasma-optical emission spectrometry

    Energy Technology Data Exchange (ETDEWEB)

    Avila Orozco, Francisco D. [Lab. FIA, Sección Química Analítica, INQUISUR (UNS-CONICET), Universidad Nacional del Sur, Avenida Alem 1253, B8000CPB, Bahía Blanca (Argentina); Kovachev, Nikolay; Aguirre Pastor, Miguel Ángel [Dpto. Química Analítica, Nutrición y Bromatología e Instituto Universitario de Materiales, Facultad de Ciencias, Universidad de Alicante, Apdo. 99, Alicante (Spain); Domini, Claudia E.; Fernández Band, Beatriz S. [Lab. FIA, Sección Química Analítica, INQUISUR (UNS-CONICET), Universidad Nacional del Sur, Avenida Alem 1253, B8000CPB, Bahía Blanca (Argentina); Canals Hernández, Antonio, E-mail: a.canals@ua.es [Dpto. Química Analítica, Nutrición y Bromatología e Instituto Universitario de Materiales, Facultad de Ciencias, Universidad de Alicante, Apdo. 99, Alicante (Spain)

    2014-05-01

    Highlights: • The elemental analysis of biodiesel by the proposed method is simple and fast. • Two-nozzles Flow Blurring® nebulizer allow to reduce the spectral interferences. • Two-nozzles Flow Blurring® nebulizer avoid the formation of carbon deposits. • The analysis may be carried out without any sample pretreatment. Abstract: A simple and fast method for determining the content of Na, K, Ca, Mg, P, and 20 heavy metals in biodiesel samples with inductively coupled plasma optical emission spectrometry (ICP OES) using a two-nozzle Flow Blurring® multinebulizer prototype and on-line internal standard calibration, are proposed. The biodiesel samples were produced from different feedstock such as sunflower, corn, soybean and grape seed oils, via a base catalyst transesterification. The analysis was carried out without any sample pretreatment. The standards and samples were introduced through one of the multinebulizer nozzles, while the aqueous solution containing yttrium as an internal standard was introduced through the second nozzle. Thus, the spectral interferences were compensated and the formation of carbon deposits on the ICP torch was prevented. The determination coefficients (R²) were greater than 0.99 for the studied analytes, in the range 0.21–14.75 mg kg⁻¹. Short-term and long-term precisions were estimated as relative standard deviation. These were acceptable, their values being lower than 10%. The LOQ for major components such as Ca, K, Mg, Na, and P, were within a range between 4.9 ng g⁻¹ for Mg (279.553 nm) and 531.1 ng g⁻¹ for Na (588.995 nm), and for the other 20 minor components they were within a range between 1.1 ng g⁻¹ for Ba (455.403 nm) and 2913.9 ng g⁻¹ for Pb (220.353 nm). Recovery values ranged between 95% and 106%.

  9. Instrumental analysis

    International Nuclear Information System (INIS)

    This textbook deals with instrumental analysis, which consists of nine chapters. It has Introduction of analysis chemistry, the process of analysis and types and form of the analysis, Electrochemistry on basic theory, potentiometry and conductometry, electromagnetic radiant rays and optical components on introduction and application, Ultraviolet rays and Visible spectrophotometry, Atomic absorption spectrophotometry on introduction, flame emission spectrometry and plasma emission spectrometry. The others like infrared spectrophotometry, X-rays spectrophotometry and mass spectrometry, chromatography and the other instrumental analysis like radiochemistry.

  10. Pilipino Kindergarten Language Arts--Needs. Teacher's Handbook [and] Teacher's Answer Key [and] Student Workbook=Sining ng Wika Pangkindergarten--Mga Pangangailangan. Hanbuk ng Guro.

    Science.gov (United States)

    California State Univ., Los Angeles. National Dissemination and Assessment Center.

    As part of the Asian American Bilingual Center (AABC) Pilipino Language Arts Program, a curriculum suitable for students in kindergarten is presented. The program is geared both to the immigrant Filipino child and to the American-born Filipino child who learned to speak Pilipino at home, and includes kits that emphasize the AABC's major concepts:…

  11. 76 FR 27861 - Airworthiness Directives; Diamond Aircraft Industries GmbH Models DA 42, DA 42 NG, and DA 42 M-NG...

    Science.gov (United States)

    2011-05-13

    ... (76 FR 12627). That NPRM proposed to correct an unsafe condition for the specified products. The MCAI...-16534 (75 FR 75868, December 7, 2010), as a unilateral action to address this unsafe condition on Models... Regulatory Policies and Procedures (44 FR 11034, February 26, 1979); and (3) Will not have a...

  12. Safety and immunogenicity of hepatitis B vaccine ButaNG in adults Inocuidade e imunogenicidade da vacina contra a hepatite B, ButaNG, em adultos

    Directory of Open Access Journals (Sweden)

    Luzia M. IOSHIMOTO

    1999-05-01

    Full Text Available Recombinant yeast-derived hepatitis B vaccine manufactured by Instituto Butantan was administered in two groups of adult volunteers (I, II following two different schedules of immunization. In the first trial (10 mg doses and 0, 1, 3 months vaccination schedule 106 individuals completed the full immunization program. The results of seroconversion by age group varied from 70 to 100% and the GMT from 46.5 to 124.9 mIU mL-1. In the second trial with 68 individuals (for dosage comparison and 0, 1, 6 months vaccination schedule indicated that the vaccine formulated in 20 mg was more effective than in 10 mg. The adverse reactions observed in the vaccinees were less frequent than the ones previously found since the introduction of similar vaccines.Vacina contra a hepatite B, produzida no Instituto Butantan em levedura recombinante, foi administrada em dois grupos de voluntários adultos (I e II seguindo dois esquemas diferentes de imunização. No primeiro ensaio (doses de 10 mg e esquema de vacinação de 0, 1, 3 meses, 106 indivíduos completaram o programa de imunização proposto. Os resultados de soroconversão agrupados por faixa etária variaram de 70 a 100%, enquanto que o TGM foi de 46,5 a 124 mUI mL-1. No segundo ensaio com 68 indivíduos (comparação de doses e esquema de vacinação de 0, 1, 6 meses os resultados indicaram que a vacina formulada em dose de 20 mg foi mais eficaz que em dose de 10 mg. Os efeitos adversos observados nos vacinados foram menores do que os relatados por outros autores, desde o início da aplicação de vacinas similares.

  13. Speciation neutron activation analysis for arsenic in marine fish

    International Nuclear Information System (INIS)

    Neutron activation analysis (NAA) methods involving chemical separations prior to irradiations can be developed to determine the species of an element. The technique can be called speciation NAA (SNAA). We have developed SNAA methods for assaying various arsenic species, namely As(III), As(V), dimethyl arsonic acid (DMA), monomethylarsinic acid (MMA), arsenobetaine (AsB), organically bound arsenic (OBAs), and lipid-soluble arsenic (LSAs) in marine fish samples. The method involves extraction by a methanol-MIBK-water system, cation exchange chromatography, and HPLC followed by NAA. The detection limits for various arsenic species are around 20 ng g-1 of fish under the experimental conditions of 2-h irradiation in a neutron flux of 5x1011 cm-2 s-1 at the Dalhousie University SLOWPOKE-2 facility. Details of the methods and results are presented

  14. Calibration of a PIXE system for analysis of environmental aerosols

    International Nuclear Information System (INIS)

    The results of the calibration of the PIXE system for analysis of environmental aerosols in the CNM is presented. The system was calibrated in order to lead to the project study of environmental aerosols using PIXE from the qualitative dimension to the quantitative one. Part of this work it was carried out in collaboration with the expert of the IAEA Dr. D. Crumpton. The calibration of the system it was achieved bombarding thin targets of well-known composition with protons of 2.5 MeV. Using this technique it was determined the absolute elementary concentration in samples of atmospheric aerosols. As an example the calculation of the lead concentration in one of them whose result was of 662 ± 49 ng m-3 is presented. This value agrees with the measurements made in the Crocker Nuclear Lab of the UCD. Its are also discussed the reproducibility and precision of the used technique. (Author)

  15. Supported liquid extraction (SLE) for the analysis of methylamphetamine, methylenedioxymethylamphetamine and delta-9-tetrahydrocannabinol in oral fluid and blood of drivers.

    Science.gov (United States)

    Rositano, Joanna; Harpas, Peter; Kostakis, Chris; Scott, Timothy

    2016-08-01

    Since 2006, the South Australian Government has been conducting roadside oral fluid testing of drivers for the illicit drugs methylamphetamine (MA), methylenedioxymethylamphetamine (MDMA) and Δ(9)-tetrahydrocannabinol (THC) using the Securetec Drugwipe II Twin and Alere DDS 805 AP saliva collection kit. Forensic Science South Australia carries out the confirmatory analysis by LC/MS for the positive test results of oral fluid roadside testing along with the pre-screened ELISA positive road traffic accident blood samples. The number of blood and oral fluid samples received in the laboratory has been steadily increasing during this time, and over 10,000 samples were received in 2014. The proportion of positive results from these samples has also been increasing over the decade of driver drug testing, and this data is presented. A simple and efficient method has been developed for the analysis of the three drugs using Biotage Isolute(®) SLE+ 96-well plates. Sample preparation included 1:1 dilution with a dilute ammonia solution for buffered oral fluids (1:3 dilution for blood samples), and addition of deuterated internal standards. Samples were loaded onto the phase, left to absorb for 5min then eluted with methyl t-butyl ether (MTBE). The samples were evaporated and reconstituted in methanol. LC/MS analysis was performed on an AB Sciex 5500 Q-Trap in positive ion mode, monitoring 3 transitions for each analyte. Separation was achieved on a Restek Ultrabiphenyl 50×2.1mm column with a gradient system of acetonitrile/0.1% formic acid over 5min. Method validation and recoveries were carried out on drug free ante mortem blood and DDS buffer solution provided by Alere, Australia. Recoveries above 80% were achieved for MA and MDMA at a concentration of 25ng/mL, whilst recoveries of greater than 65% were achieved for THC at 4.5ng/mL. Accuracy and precision were acceptable down to the LLOQ for all three analytes (5, 5 and 1ng/mL for MA, MDMA and THC, respectively). Mean

  16. Optimizing sample pretreatment for compound-specific stable carbon isotopic analysis of amino sugars in marine sediment

    Directory of Open Access Journals (Sweden)

    R. Zhu

    2014-01-01

    Full Text Available Amino sugars are quantitatively significant constituents of soil and marine sediment, but their sources and turnover in environmental samples remain poorly understood. The stable carbon isotopic composition of amino sugars can provide information on the lifestyles of their source organisms and can be monitored during incubations with labeled substrates to estimate the turnover rates of microbial populations. However, until now, such investigation has been carried out only with soil samples, partly because of the much lower abundance of amino sugars in marine environments. We therefore optimized a procedure for compound-specific isotopic analysis of amino sugars in marine sediment employing gas chromatography-isotope ratio mass spectrometry. The whole procedure consisted of hydrolysis, neutralization, enrichment, and derivatization of amino sugars. Except for the derivatization step, the protocol introduced negligible isotopic fractionation, and the minimum requirement of amino sugar for isotopic analysis was 20 ng, i.e. equivalent to ~ 8 ng of amino sugar carbon. Our results obtained from δ13C analysis of amino sugars in selected marine sediment samples showed that muramic acid had isotopic imprints from indigenous bacterial activities, whereas glucosamine and galactosamine were mainly derived from organic detritus. The analysis of stable carbon isotopic compositions of amino sugars opens a promising window for the investigation of microbial metabolisms in marine sediments and the deep marine biosphere.

  17. Optimizing sample pretreatment for compound-specific stable carbon isotopic analysis of amino sugars in marine sediment

    Science.gov (United States)

    Zhu, R.; Lin, Y.-S.; Lipp, J. S.; Meador, T. B.; Hinrichs, K.-U.

    2014-09-01

    Amino sugars are quantitatively significant constituents of soil and marine sediment, but their sources and turnover in environmental samples remain poorly understood. The stable carbon isotopic composition of amino sugars can provide information on the lifestyles of their source organisms and can be monitored during incubations with labeled substrates to estimate the turnover rates of microbial populations. However, until now, such investigation has been carried out only with soil samples, partly because of the much lower abundance of amino sugars in marine environments. We therefore optimized a procedure for compound-specific isotopic analysis of amino sugars in marine sediment, employing gas chromatography-isotope ratio mass spectrometry. The whole procedure consisted of hydrolysis, neutralization, enrichment, and derivatization of amino sugars. Except for the derivatization step, the protocol introduced negligible isotopic fractionation, and the minimum requirement of amino sugar for isotopic analysis was 20 ng, i.e., equivalent to ~8 ng of amino sugar carbon. Compound-specific stable carbon isotopic analysis of amino sugars obtained from marine sediment extracts indicated that glucosamine and galactosamine were mainly derived from organic detritus, whereas muramic acid showed isotopic imprints from indigenous bacterial activities. The δ13C analysis of amino sugars provides a valuable addition to the biomarker-based characterization of microbial metabolism in the deep marine biosphere, which so far has been lipid oriented and biased towards the detection of archaeal signals.

  18. Double-ion imprinted polymer @magnetic nanoparticles modified screen printed carbon electrode for simultaneous analysis of cerium and gadolinium ions

    Energy Technology Data Exchange (ETDEWEB)

    Prasad, Bhim Bali, E-mail: prof.bbpd@yahoo.com; Jauhari, Darshika

    2015-05-22

    Highlights: • Synthesis of a double-ion imprinted polymer for analysis of Ce(IV) and Gd(III). • Imprinted nano-beads were grown on MNPs-modified SPCE surface. • Voltammetric determination of both templates was carried out simultaneously. • Ultra-trace analysis with LOD (ng mL{sup −1}) 0.07 for Ce(IV) and 0.19 for Gd(III) is achieved. - Abstract: A typical, reproducible, and rugged screen printed carbon electrode, modified with dual-ion imprinted beads, was fabricated employing the “surface grafting from” approach. For this, the acyl chloride functionalized magnetic nanoparticles were first immobilized and chemically attached with a typical functional monomer (but-2-enedioic acid bis-[(2-amino-ethyl)-amide]) on the electrode surface. This was subsequently subjected to the thermal polymerization in the presence of template ions (Ce(IV) and Gd(III)), cross-linker (ethylene glycol dimethacrylate), initiator (AIBN), and multiwalled carbon nanotubes. The modified sensor was used for the simultaneous analysis of both template ions in aqueous, blood serum, and waste-water samples, using differential pulse anodic stripping voltammetry which revealed two oxidation peaks for respective templates with resolution as much as 950 mV, without any cross reactivity, interferences and false-positives. The detection limits realized by the proposed sensor, under optimized conditions, were found to be as low as 0.07 ng mL{sup −1} for Ce(IV) and 0.19 ng mL{sup −1} for Gd(III) (S/N = 3) that could eventually be helpful for lanthanide estimation at stringent levels.

  19. Permanent interstitial low-dose-rate brachytherapy for patients with low risk prostate cancer. An interim analysis of 312 cases

    International Nuclear Information System (INIS)

    The biochemical relapse-free survival (bRFS) rate after treatment with permanent iodine-125 seed implantation (PSI) or combined seeds and external beam radiotherapy (COMB) for clinical stage T1-T2 localized prostate cancer is a clinically relevant endpoint. The goal of this work was to evaluate the influence of relevant patient- and treatment-related factors. The study population comprised 312 consecutive patients treated with permanent seed implantation. All patients were evaluable for analysis of overall survival (OS) and disease-specific survival (DSS), 230 for bRFS, of which 192 were in the PSI group and 38 in the COMB group. The prescribed minimum peripheral dose was 145 Gy for PSI, for COMB 110 Gy implant and external beam radiotherapy of 45 Gy. The median follow-up time was 33 months (range 8-66 months). bRFS was defined as a serum prostate-specific antigen (PSA) level ≤ 0.2 ng/ml at last follow-up. Overall, the actuarial bRFS at 50 months was 88.4 %. The 50-month bRFS rate for PSI and COMB was 90.9 %, and 77.2 %, respectively. In the univariate analysis, age in the categories ≤ 63 and > 63 years (p < 0.00), PSA nadir (≤ 0.5 ng/ml and > 0.5 ng/ml) and PSA bounce (yes/no) were the significant predicting factors for bRFS. None of the other patient and treatment variables (treatment modality, stage, PSA, Gleason score, risk group, number of risk factors, D90 and various other dose parameters) were found to be a statistically significant predictor of 50-month bRFS. The biochemical failure rates were low in this study. As a proof of principle, our large monocenteric analysis shows that low-dose-rate brachytherapy is an effective and safe procedure for patients with early stage prostate cancer. (orig.)

  20. Double-ion imprinted polymer @magnetic nanoparticles modified screen printed carbon electrode for simultaneous analysis of cerium and gadolinium ions

    International Nuclear Information System (INIS)

    Highlights: • Synthesis of a double-ion imprinted polymer for analysis of Ce(IV) and Gd(III). • Imprinted nano-beads were grown on MNPs-modified SPCE surface. • Voltammetric determination of both templates was carried out simultaneously. • Ultra-trace analysis with LOD (ng mL−1) 0.07 for Ce(IV) and 0.19 for Gd(III) is achieved. - Abstract: A typical, reproducible, and rugged screen printed carbon electrode, modified with dual-ion imprinted beads, was fabricated employing the “surface grafting from” approach. For this, the acyl chloride functionalized magnetic nanoparticles were first immobilized and chemically attached with a typical functional monomer (but-2-enedioic acid bis-[(2-amino-ethyl)-amide]) on the electrode surface. This was subsequently subjected to the thermal polymerization in the presence of template ions (Ce(IV) and Gd(III)), cross-linker (ethylene glycol dimethacrylate), initiator (AIBN), and multiwalled carbon nanotubes. The modified sensor was used for the simultaneous analysis of both template ions in aqueous, blood serum, and waste-water samples, using differential pulse anodic stripping voltammetry which revealed two oxidation peaks for respective templates with resolution as much as 950 mV, without any cross reactivity, interferences and false-positives. The detection limits realized by the proposed sensor, under optimized conditions, were found to be as low as 0.07 ng mL−1 for Ce(IV) and 0.19 ng mL−1 for Gd(III) (S/N = 3) that could eventually be helpful for lanthanide estimation at stringent levels