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Sample records for analysis reveals convergent

  1. A comparative mechanical analysis of plant and animal cells reveals convergence across kingdoms.

    Science.gov (United States)

    Durand-Smet, Pauline; Chastrette, Nicolas; Guiroy, Axel; Richert, Alain; Berne-Dedieu, Annick; Szecsi, Judit; Boudaoud, Arezki; Frachisse, Jean-Marie; Bendahmane, Mohammed; Bendhamane, Mohammed; Hamant, Oliver; Asnacios, Atef

    2014-11-18

    Plant and animals have evolved different strategies for their development. Whether this is linked to major differences in their cell mechanics remains unclear, mainly because measurements on plant and animal cells relied on independent experiments and setups, thus hindering any direct comparison. In this study we used the same micro-rheometer to compare animal and plant single cell rheology. We found that wall-less plant cells exhibit the same weak power law rheology as animal cells, with comparable values of elastic and loss moduli. Remarkably, microtubules primarily contributed to the rheological behavior of wall-less plant cells whereas rheology of animal cells was mainly dependent on the actin network. Thus, plant and animal cells evolved different molecular strategies to reach a comparable cytoplasmic mechanical core, suggesting that evolutionary convergence could include the internal biophysical properties of cells.

  2. Genomic convergence analysis of schizophrenia: mRNA sequencing reveals altered synaptic vesicular transport in post-mortem cerebellum.

    Directory of Open Access Journals (Sweden)

    Joann Mudge

    Full Text Available Schizophrenia (SCZ is a common, disabling mental illness with high heritability but complex, poorly understood genetic etiology. As the first phase of a genomic convergence analysis of SCZ, we generated 16.7 billion nucleotides of short read, shotgun sequences of cDNA from post-mortem cerebellar cortices of 14 patients and six, matched controls. A rigorous analysis pipeline was developed for analysis of digital gene expression studies. Sequences aligned to approximately 33,200 transcripts in each sample, with average coverage of 450 reads per gene. Following adjustments for confounding clinical, sample and experimental sources of variation, 215 genes differed significantly in expression between cases and controls. Golgi apparatus, vesicular transport, membrane association, Zinc binding and regulation of transcription were over-represented among differentially expressed genes. Twenty three genes with altered expression and involvement in presynaptic vesicular transport, Golgi function and GABAergic neurotransmission define a unifying molecular hypothesis for dysfunction in cerebellar cortex in SCZ.

  3. Convergence analysis of cautious control

    Institute of Scientific and Technical Information of China (English)

    ZHANG Yanxia; GUO Lei

    2006-01-01

    In this paper, we present a theoretical analysis on stability and convergence of the cautious control, which has advantages over the traditional certainty equivalence adaptive control, since it takes the parameter estimation error into account in the design,and is also one-step-ahead optimal in the mean square sense under Gaussian assumptions.

  4. Convergence analysis of combinations of different methods

    Energy Technology Data Exchange (ETDEWEB)

    Kang, Y. [Clarkson Univ., Potsdam, NY (United States)

    1994-12-31

    This paper provides a convergence analysis for combinations of different numerical methods for solving systems of differential equations. The author proves that combinations of two convergent linear multistep methods or Runge-Kutta methods produce a new convergent method of which the order is equal to the smaller order of the two original methods.

  5. Core Backbone Convergence Mechanisms and Microloops Analysis

    Directory of Open Access Journals (Sweden)

    Abdelali Ala

    2012-07-01

    Full Text Available In this article we study approaches that can be used to minimise the convergence time, we also make a focus on microloops phenomenon, analysis and means to mitigate them. The convergence time reflects the time required by a network to react to a failure of a link or a router failure itself. When all nodes (routers have updated their respective routing and forwarding databases, we can say the network has converged. This study will help in building real-time and resilient network infrastructure, the goal is to make any evenement in the core network, as transparent as possible to any sensitive and real-time flows. This study is also, a deepening of earlier works presented in [10] and [11].

  6. Convergence

    Science.gov (United States)

    Darcie, Thomas E.; Doverspike, Robert; Zirngibl, Martin; Korotky, Steven K.

    2005-09-01

    Call for Papers: Convergence The Journal of Optical Networking (JON) invites submissions to a special issue on Convergence. Convergence has become a popular theme in telecommunications, one that has broad implications across all segments of the industry. Continual evolution of technology and applications continues to erase lines between traditionally separate lines of business, with dramatic consequences for vendors, service providers, and consumers. Spectacular advances in all layers of optical networking-leading to abundant, dynamic, cost-effective, and reliable wide-area and local-area connections-have been essential drivers of this evolution. As services and networks continue to evolve towards some notion of convergence, the continued role of optical networks must be explored. One vision of convergence renders all information in a common packet (especially IP) format. This vision is driven by the proliferation of data services. For example, time-division multiplexed (TDM) voice becomes VoIP. Analog cable-television signals become MPEG bits streamed to digital set-top boxes. T1 or OC-N private lines migrate to Ethernet virtual private networks (VPNs). All these packets coexist peacefully within a single packet-routing methodology built on an optical transport layer that combines the flexibility and cost of data networks with telecom-grade reliability. While this vision is appealing in its simplicity and shared widely, specifics of implementation raise many challenges and differences of opinion. For example, many seek to expand the role of Ethernet in these transport networks, while massive efforts are underway to make traditional TDM networks more data friendly within an evolved but backward-compatible SDH/SONET (synchronous digital hierarchy and synchronous optical network) multiplexing hierarchy. From this common underlying theme follow many specific instantiations. Examples include the convergence at the physical, logical, and operational levels of voice and

  7. Convergence

    DEFF Research Database (Denmark)

    Prasad, Ramjee

    2009-01-01

    This paper presents the main conclusions which can be drawn from the discussions on Future Communication Systems and lessons on Unpredictable Future of Wireless Communication Systems. Future systems beyond the third generation are already under discussions in international bodies, such as ITU, WW...... and R&D programmes worldwide. The incoming era is characterized by the convergence of networks and access technology and the divergence of applications. Future mobile communication systems should bring something more than only faster data or wireless internet access....

  8. Convergence Analysis for the Multiplicative Schwarz Preconditioned Inexact Newton Algorithm

    KAUST Repository

    Liu, Lulu

    2016-10-26

    The multiplicative Schwarz preconditioned inexact Newton (MSPIN) algorithm, based on decomposition by field type rather than by subdomain, was recently introduced to improve the convergence of systems with unbalanced nonlinearities. This paper provides a convergence analysis of the MSPIN algorithm. Under reasonable assumptions, it is shown that MSPIN is locally convergent, and desired superlinear or even quadratic convergence can be obtained when the forcing terms are picked suitably.

  9. Distributed Beamforming with Feedback: Convergence Analysis

    CERN Document Server

    Lin, C; Meyn, S

    2008-01-01

    The focus of this work is on the analysis of transmit beamforming schemes with a low-rate feedback link in wireless sensor/relay networks, where nodes in the network need to implement beamforming in a distributed manner. Specifically, the problem of distributed phase alignment is considered, where neither the transmitters nor the receiver has perfect channel state information, but there is a low-rate feedback link from the receiver to the transmitters. In this setting, a framework for systematically analyzing the performance of a general set of distributed beamforming schemes is proposed. To illustrate the advantage of this framework, a simple adaptive distributed beamforming scheme that was recently proposed by Mudambai et al. is studied. Two important properties for the received signal magnitude function are derived. Using these properties and the systematic framework, it is shown that the adaptive distributed beamforming scheme converges both in probability and in mean. Furthermore, it is established that ...

  10. Convergence

    DEFF Research Database (Denmark)

    Madsen, Ole Brun; Nielsen, Jens Frederik Dalsgaard; Schiøler, Henrik

    2002-01-01

    Convergence trends between the WAN Internet area, characterized by best effort service provision, and the real time LAN domain, with requirements for guaranteed services, are identified and discussed. A bilateral evolution is identified, where typical bulk service applications from WAN, such as m......Convergence trends between the WAN Internet area, characterized by best effort service provision, and the real time LAN domain, with requirements for guaranteed services, are identified and discussed. A bilateral evolution is identified, where typical bulk service applications from WAN...... with the emergence of remote service provision, such as supervision and control of decentralized heating facilities and wind based electrical power production. The reliability issue is addressed from a structural viewpoint, where the concept of Structural QoS (SQoS) is defined to support reliability modelling...... in communication infrastructures. A graph theoretical approach is presented as an approach to reliability management in complex communication infrastructures. Real life examples are provided and specific problems are presented and discussed. Wireless technologies are discussed as a complement, providing not only...

  11. Characteristic analysis and prevention on premature convergence in genetic algorithms

    Institute of Scientific and Technical Information of China (English)

    徐宗本; 高勇

    1997-01-01

    The identification and characteristics of premature convergence in genetic algorithms (GAs) are investigated Through a detailed quantitative analysis on the search capability and the degree of population diversity, the cause of premature convergence in GAs is recognized, and attributed to the maturation effect of the GAs: The minimum schema deduced from current population, which is the largest search space of a GA, converges to a homogeneous population in probability 1 ( so the search capability of the GA decreases and premature convergence occurs). It is shown that, as quantitative features of the maturation effect, the degree of population diversity converges to zero with probability 1, and the tendency for premature convergence is inversely proportional to the population size and directly proportional to the variance of the fitness ratio of zero allele at any gene position of the current population. Based on the theoretical analysis, several strategies for preventing premature convergence are suggest

  12. Convergence and Rate Analysis of Neural Networks for Sparse Approximation

    CERN Document Server

    Balavoine, Aurèle; Rozell, Christopher J

    2011-01-01

    We present an analysis of the Locally Competitive Algorithm (LCA), a Hopfield-style neural network that solves sparse approximation problems (e.g., approximating a vector from a dictionary using just a few non-zero coefficients). This class of problems plays a significant role in both theories of neural coding and applications in signal processing, but traditional analysis approaches are difficult because the objective functions are non-smooth. Specifically, we characterize the convergence properties of this system by showing that the LCA is globally convergent to a fixed point corresponding to the exact solution of the objective function, and (under some mild conditions) this solution is reached in finite time. Furthermore, we characterize the convergence rate of the system by showing that the LCA converges exponentially fast with an analytically bounded convergence rate (that depends on the specifics of a given problem). We support our analysis with several illustrative simulations.

  13. Convergence of algorithms used for principal component analysis

    Institute of Scientific and Technical Information of China (English)

    张俊华; 陈翰馥

    1997-01-01

    The convergence of algorithms used for principal component analysis is analyzed. The algorithms are proved to converge to eigenvectors and eigenvalues of a matrix A which is the expectation of observed random samples. The conditions required here are considerably weaker than those used in previous work.

  14. The analysis of the convergence of ant colony optimization algorithm

    Institute of Scientific and Technical Information of China (English)

    ZHU Qingbao; WANG Lingling

    2007-01-01

    The ant colony optimization algorithm has been widely studied and many important results have been obtained.Though this algorithm has been applied to many fields.the analysis about its convergence is much less,which will influence the improvement of this algorithm.Therefore,the convergence of this algorithm applied to the traveling salesman problem(TSP)was analyzed in detail.The conclusion that this algorithm will definitely converge to the optimal solution under the condition of 0<q0<1 was proved true.In addition,the influence on its convergence caused by the properties of the closed path,heuristic functions,the pheromone and q0 was analyzed.Based on the above-mentioned,some conclusions about how to improve the speed of its convergence are obtained.

  15. Convergence analysis of Strang splitting for Vlasov-type equations

    CERN Document Server

    Einkemmer, Lukas

    2012-01-01

    A rigorous convergence analysis of the Strang splitting algorithm for Vlasov-type equations in the setting of abstract evolution equations is provided. It is shown that under suitable assumptions the convergence is of second order in the time step h. As an example, it is verified that the Vlasov-Poisson equation in 1+1 dimensions fits into the framework of this analysis. Also, numerical experiments for the latter case are presented.

  16. Convergence Analysis of a Domain Decomposition Paradigm

    Energy Technology Data Exchange (ETDEWEB)

    Bank, R E; Vassilevski, P S

    2006-06-12

    We describe a domain decomposition algorithm for use in several variants of the parallel adaptive meshing paradigm of Bank and Holst. This algorithm has low communication, makes extensive use of existing sequential solvers, and exploits in several important ways data generated as part of the adaptive meshing paradigm. We show that for an idealized version of the algorithm, the rate of convergence is independent of both the global problem size N and the number of subdomains p used in the domain decomposition partition. Numerical examples illustrate the effectiveness of the procedure.

  17. Assessing the validity of discourse analysis: transdisciplinary convergence

    Science.gov (United States)

    Jaipal-Jamani, Kamini

    2014-12-01

    Research studies using discourse analysis approaches make claims about phenomena or issues based on interpretation of written or spoken text, which includes images and gestures. How are findings/interpretations from discourse analysis validated? This paper proposes transdisciplinary convergence as a way to validate discourse analysis approaches to research. The argument is made that discourse analysis explicitly grounded in semiotics, systemic functional linguistics, and critical theory, offers a credible research methodology. The underlying assumptions, constructs, and techniques of analysis of these three theoretical disciplines can be drawn on to show convergence of data at multiple levels, validating interpretations from text analysis.

  18. Comparative Convergence Analysis of Nonlinear AMLI-Cycle Multigrid

    Energy Technology Data Exchange (ETDEWEB)

    Hu, Xiaozhe [Pennsylvania State Univ., University Park, PA (United States). Dept. of Mathematics; Vassilevski, Panayot S. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Xu, Jinchao [Pennsylvania State Univ., University Park, PA (United States). Dept. of Mathematics

    2013-04-30

    The purpose of our paper is to provide a comprehensive convergence analysis of the nonlinear algebraic multilevel iteration (AMLI)-cycle multigrid (MG) method for symmetric positive definite problems. We show that the nonlinear AMLI-cycle MG method is uniformly convergent, based on classical assumptions for approximation and smoothing properties. Furthermore, under only the assumption that the smoother is convergent, we show that the nonlinear AMLI-cycle method is always better (or not worse) than the respective V-cycle MG method. Finally, numerical experiments are presented to illustrate the theoretical results.

  19. Financial Convergence Analysis: Implication for Insurance and Pension Markets

    Directory of Open Access Journals (Sweden)

    Natalia P. Kuznetsova

    2016-06-01

    Full Text Available The proposed paper is one of a set of articles dedicated to the new phenomenon in the global and national financial markets – financial convergence – and is focused on theoretical issues. The hypothesis of the article is to argue whether the financial convergence determines the directions of financial market (namely, insurance and pension sectors development. Adequately the goal of this paper is to analyze the existence of convergence processes in the insurance and pension markets. Methods of systematic and logical analysis are used. In the first part authors give brief history of the convergence phenomenon research. Then the paper analyses influence of financial convergence on insurance and pension markets, manifested in the following effects: mix of financial institutions functions; distribution channels advantages, increase of insurance and pension funds companies’ competitiveness; governance models convergence. The major results of the study are: demographic shifts in different developed and emerging markets countries caused the need to reform the social security systems and public pension schemes and refocus them to the market-based financial convergence model; pension funds, acting as institutional investors, are the leading players in the contemporary global financial market; competition at the financial market causes the expansion of a number of services offered by various organizations: banks, insurance companies, pension funds and so on, which offer a wide range of services not directly related to their core businesses; the mixing of financial institutions functions from the insurance, pension and banking sectors, increased competition for customers at the national and global financial market.

  20. Assessing the Validity of Discourse Analysis: Transdisciplinary Convergence

    Science.gov (United States)

    Jaipal-Jamani, Kamini

    2014-01-01

    Research studies using discourse analysis approaches make claims about phenomena or issues based on interpretation of written or spoken text, which includes images and gestures. How are findings/interpretations from discourse analysis validated? This paper proposes transdisciplinary convergence as a way to validate discourse analysis approaches to…

  1. Convergence Analysis of a Class of Computational Intelligence Approaches

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    Junfeng Chen

    2013-01-01

    Full Text Available Computational intelligence approaches is a relatively new interdisciplinary field of research with many promising application areas. Although the computational intelligence approaches have gained huge popularity, it is difficult to analyze the convergence. In this paper, a computational model is built up for a class of computational intelligence approaches represented by the canonical forms of generic algorithms, ant colony optimization, and particle swarm optimization in order to describe the common features of these algorithms. And then, two quantification indices, that is, the variation rate and the progress rate, are defined, respectively, to indicate the variety and the optimality of the solution sets generated in the search process of the model. Moreover, we give four types of probabilistic convergence for the solution set updating sequences, and their relations are discussed. Finally, the sufficient conditions are derived for the almost sure weak convergence and the almost sure strong convergence of the model by introducing the martingale theory into the Markov chain analysis.

  2. Revealing the basins of convergence in the planar equilateral restricted four-body problem

    Science.gov (United States)

    Zotos, Euaggelos E.

    2017-01-01

    The planar equilateral restricted four-body problem where two of the primaries have equal masses is used in order to determine the Newton-Raphson basins of convergence associated with the equilibrium points. The parametric variation of the position of the libration points is monitored when the value of the mass parameter m3 varies in predefined intervals. The regions on the configuration (x,y) plane occupied by the basins of attraction are revealed using the multivariate version of the Newton-Raphson iterative scheme. The correlations between the attracting domains of the equilibrium points and the corresponding number of iterations needed for obtaining the desired accuracy are also illustrated. We perform a thorough and systematic numerical investigation by demonstrating how the dynamical parameter m3 influences the shape, the geometry and the degree of fractality of the converging regions. Our numerical outcomes strongly indicate that the mass parameter is indeed one of the most influential factors in this dynamical system.

  3. Investigating Convergence Patterns for Numerical Methods Using Data Analysis

    Science.gov (United States)

    Gordon, Sheldon P.

    2013-01-01

    The article investigates the patterns that arise in the convergence of numerical methods, particularly those in the errors involved in successive iterations, using data analysis and curve fitting methods. In particular, the results obtained are used to convey a deeper level of understanding of the concepts of linear, quadratic, and cubic…

  4. Convergence of the discrete dipole approximation. I. Theoretical analysis

    CERN Document Server

    Yurkin, Maxim A; Hoekstra, Alfons G

    2006-01-01

    We performed a rigorous theoretical convergence analysis of the discrete dipole approximation (DDA). We prove that errors in any measured quantity are bounded by a sum of a linear and quadratic term in the size of a dipole d, when the latter is in the range of DDA applicability. Moreover, the linear term is significantly smaller for cubically than for non-cubically shaped scatterers. Therefore, for small d errors for cubically shaped particles are much smaller than for non-cubically shaped. The relative importance of the linear term decreases with increasing size, hence convergence of DDA for large enough scatterers is quadratic in the common range of d. Extensive numerical simulations were carried out for a wide range of d. Finally we discuss a number of new developments in DDA and their consequences for convergence.

  5. Simulation and Analysis of Converging Shock Wave Test Problems

    Energy Technology Data Exchange (ETDEWEB)

    Ramsey, Scott D. [Los Alamos National Laboratory; Shashkov, Mikhail J. [Los Alamos National Laboratory

    2012-06-21

    Results and analysis pertaining to the simulation of the Guderley converging shock wave test problem (and associated code verification hydrodynamics test problems involving converging shock waves) in the LANL ASC radiation-hydrodynamics code xRAGE are presented. One-dimensional (1D) spherical and two-dimensional (2D) axi-symmetric geometric setups are utilized and evaluated in this study, as is an instantiation of the xRAGE adaptive mesh refinement capability. For the 2D simulations, a 'Surrogate Guderley' test problem is developed and used to obviate subtleties inherent to the true Guderley solution's initialization on a square grid, while still maintaining a high degree of fidelity to the original problem, and minimally straining the general credibility of associated analysis and conclusions.

  6. Nonstandard analysis and jump conditions for converging shock waves

    Science.gov (United States)

    Baty, Roy S.; Farassat, F.; Tucker, Don H.

    2008-06-01

    Nonstandard analysis is an area of modern mathematics that studies abstract number systems containing both infinitesimal and infinite numbers. This article applies nonstandard analysis to derive jump conditions for one-dimensional, converging shock waves in a compressible, inviscid, perfect gas. It is assumed that the shock thickness occurs on an infinitesimal interval and the jump functions in the thermodynamic and fluid dynamic parameters occur smoothly across this interval. Predistributions of the Heaviside function and the Dirac delta measure are introduced to model the flow parameters across a shock wave. The equations of motion expressed in nonconservative form are then applied to derive unambiguous relationships between the jump functions for the flow parameters.

  7. An improved convergence analysis of smoothed aggregation algebraic multigrid

    Energy Technology Data Exchange (ETDEWEB)

    Brezina, Marian [Univ. of Colorado, Boulder, CO (United States). Dept. of Applied Mathematics; Vaněk, Petr [University of West Bohemia (Czech Republic). Dept. of Mathematics; Vassilevski, Panayot S. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States). Center for Applied Scientific Computing

    2011-03-02

    We present an improved analysis of the smoothed aggregation (SA) alge- braic multigrid method (AMG) extending the original proof in [SA] and its modification in [Va08]. The new result imposes fewer restrictions on the aggregates that makes it eas- ier to verify in practice. Also, we extend a result in [Van] that allows us to use aggressive coarsening at all levels due to the special properties of the polynomial smoother, that we use and analyze, and thus provide a multilevel convergence estimate with bounds independent of the coarsening ratio.

  8. Patent Network Analysis and Quadratic Assignment Procedures to Identify the Convergence of Robot Technologies

    Science.gov (United States)

    Lee, Woo Jin; Lee, Won Kyung

    2016-01-01

    Because of the remarkable developments in robotics in recent years, technological convergence has been active in this area. We focused on finding patterns of convergence within robot technology using network analysis of patents in both the USPTO and KIPO. To identify the variables that affect convergence, we used quadratic assignment procedures (QAP). From our analysis, we observed the patent network ecology related to convergence and found technologies that have great potential to converge with other robotics technologies. The results of our study are expected to contribute to setting up convergence based R&D policies for robotics, which can lead new innovation. PMID:27764196

  9. Theoretical analysis on convergence behavior of rank filters

    Institute of Scientific and Technical Information of China (English)

    YE; Wanzhou

    2004-01-01

    This paper systematically studies the convergence behavior of rank filters. The problem of convergence behavior of rank filters has been solved completely for bounded sequences. Moreover, some properties of its limiting sequences and recurrent sequences are obtained.

  10. A Study on Diffusion Pattern of Technology Convergence: Patent Analysis for Korea

    Directory of Open Access Journals (Sweden)

    Jae Young Choi

    2015-08-01

    Full Text Available Technology convergence indicates that technologies of different application areas are converted into a new and common unity of technology. Its range spans from inter-field, whereby technologies are converged between heterogeneous fields in homogeneous sector, to a wider inter-sector, whereby technologies belong to heterogeneous technology sector are converged. This paper determined the definition of technology convergence from previous literature and classified patents into technology category depending on patent information. Furthermore, we empirically measure technology convergence degree based on co-classification analysis and estimate its diffusion trend at the entire technology domain level by using overall 1,476,967 of patents filed to the KIPO (Korean Intellectual Property Office from 1998 to 2010. As a result, potential size and growth rate of technology convergence are varied by both technology and type of technology convergence, i.e., inter-field and inter-sector technology convergence. Diffusion pattern of inter-sector technology convergence appears as the more various form than that of inter-field technology convergence. In a relationship between potential size and growth rate of technology convergence, growth rate of technology convergence is in inverse proportion to potential size of technology convergence in general. That is, the faster the growth rate of technology convergence, the smaller the potential size of technology convergence. In addition, this paper found that technology convergence of the instrument and chemistry sector is actively progressing in both inter-field and inter-sector convergence, while the technologies related to Information and Communication Technology (ICT in electrical engineering sector have relatively mature progress of technology convergence, especially in inter-sector technology convergence.

  11. Convergence of Romanian accounting regulations with IFRS. A longitudinal analysis

    Directory of Open Access Journals (Sweden)

    Ioana PĂLĂRIE

    2016-06-01

    Full Text Available In this paper we analyse the evolution in the level of convergence of the Romanian accounting regulations with IFRS in the last decade. We focus our study on the accounting topics covered by IAS16, IAS17, IAS 41 and SIC15. We find that in 2005 the regulations exhibit a good level of convergence for property, plant and equipment, a medium level of convergence for lease accounting and divergence for accounting for the agricultural activity. The overall convergence level improved over time for all the topics analysed. These results indicate that the companies with dual reporting may incur lower costs in applying IFRS. Moreover, the national regulations offer the opportunity for a higher level of comparability in Romania of the financial statements prepared under IFRS with those prepared under national regulations. However, we underline that the institutional factors (such as the tax influence over accounting might negatively affect the convergence of practices.

  12. Análise de convergência espacial dos repasses da Lei Robin Hood Spatial convergence analysis of tax transfers from the Robin Hood Act

    Directory of Open Access Journals (Sweden)

    Noé Gonçalves Maranduba Júnior

    2009-12-01

    Full Text Available Alega-se que a Lei Robin Hood tem permitido uma melhor distribuição dos valores do ICMS devidos aos municípios do estado de Minas Gerais. O objetivo deste artigo é investigar se essa lei, entre os anos de 2001 e 2005, realmente foi eficaz, isto é, se, em termos relativos, municípios pobres receberam mais dessas transferências do que os municípios ricos. Para fazer isso metodologicamente, implementa-se uma análise exploratória de dados espaciais e uma análise de convergência, para verificar se as disparidades nos repasses diminuíram com o passar do tempo. Os resultados mostraram que os efeitos espaciais importaram nas análises e que não houve um efeito redistributivo dos repasses, considerando-se que o coeficiente indicador de convergência não foi significativo.One alleges that the Robin Hood Act has allowed one better distribution of the values of ICMS to municipalities of the state of Minas Gerais. The paper is aimed at verifying if the Robin Hood Act has actually revealed a redistributive effect in tax transfers to municipalities in the state over the period 2001-2005, that is, if, in relative terms, poor municipalities have received more these transfers than rich ones. In doing methodologically so, an exploratory spatial data analysis and a convergence analysis are implemented to verify if the disparities of tax redistribution have diminished over the time. The findings showed that the spatial effects were important in the analysis as well as there was no redistributive effect in the period because the convergence coefficient was not significant.

  13. Pathway Network Analyses for Autism Reveal Multisystem Involvement, Major Overlaps with Other Diseases and Convergence upon MAPK and Calcium Signaling.

    Science.gov (United States)

    Wen, Ya; Alshikho, Mohamad J; Herbert, Martha R

    2016-01-01

    We used established databases in standard ways to systematically characterize gene ontologies, pathways and functional linkages in the large set of genes now associated with autism spectrum disorders (ASDs). These conditions are particularly challenging--they lack clear pathognomonic biological markers, they involve great heterogeneity across multiple levels (genes, systemic biological and brain characteristics, and nuances of behavioral manifestations)-and yet everyone with this diagnosis meets the same defining behavioral criteria. Using the human gene list from Simons Foundation Autism Research Initiative (SFARI) we performed gene set enrichment analysis with the Kyoto Encyclopedia of Genes and Genomes (KEGG) Pathway Database, and then derived a pathway network from pathway-pathway functional interactions again in reference to KEGG. Through identifying the GO (Gene Ontology) groups in which SFARI genes were enriched, mapping the coherence between pathways and GO groups, and ranking the relative strengths of representation of pathway network components, we 1) identified 10 disease-associated and 30 function-associated pathways 2) revealed calcium signaling pathway and neuroactive ligand-receptor interaction as the most enriched, statistically significant pathways from the enrichment analysis, 3) showed calcium signaling pathways and MAPK signaling pathway to be interactive hubs with other pathways and also to be involved with pervasively present biological processes, 4) found convergent indications that the process "calcium-PRC (protein kinase C)-Ras-Raf-MAPK/ERK" is likely a major contributor to ASD pathophysiology, and 5) noted that perturbations associated with KEGG's category of environmental information processing were common. These findings support the idea that ASD-associated genes may contribute not only to core features of ASD themselves but also to vulnerability to other chronic and systemic problems potentially including cancer, metabolic conditions

  14. A simple cell-based assay reveals that diverse neuropsychiatric risk genes converge on primary cilia.

    Directory of Open Access Journals (Sweden)

    Aaron Marley

    Full Text Available Human genetic studies are beginning to identify a large number of genes linked to neuropsychiatric disorders. It is increasingly evident that different genes contribute to risk for similar syndromes and, conversely, the same genes or even the same alleles cross over traditional diagnostic categories. A current challenge is to understand the cellular biology of identified risk genes. However, most genes associated with complex neuropsychiatric phenotypes are not related through a known biochemical pathway, and many have an entirely unknown cellular function. One possibility is that diverse disease-linked genes converge at a higher-level cellular structure. The synapse is already known to be one such convergence, and emerging evidence suggests the primary cilium as another. Because many genes associated with neuropsychiatric illness are expressed also outside the nervous system, as are cilia, we tested the hypothesis that such genes affect conserved features of the primary cilium. Using RNA interference to test 41 broadly expressed candidate genes associated with schizophrenia, bipolar affective disorder, autism spectrum disorder and intellectual disability, we found 20 candidates that reduce ciliation in NIH3T3 cells when knocked down, and three whose manipulation increases cilia length. Three of the candidate genes were previously implicated in cilia formation and, altogether, approximately half of the candidates tested produced a ciliary phenotype. Our results support the hypothesis that primary cilia indeed represent a conserved cellular structure at which the effects of diverse neuropsychiatric risk genes converge. More broadly, they suggest a relatively simple cell-based approach that may be useful for exploring the complex biological underpinnings of neuropsychiatric disease.

  15. Sensitivity Analysis and Statistical Convergence of a Saltating Particle Model

    CERN Document Server

    Maldonado, S

    2016-01-01

    Saltation models provide considerable insight into near-bed sediment transport. This paper outlines a simple, efficient numerical model of stochastic saltation, which is validated against previously published experimental data on saltation in a channel of nearly horizontal bed. Convergence tests are systematically applied to ensure the model is free from statistical errors emanating from the number of particle hops considered. Two criteria for statistical convergence are derived; according to the first criterion, at least $10^3$ hops appear to be necessary for convergent results, whereas $10^4$ saltations seem to be the minimum required in order to achieve statistical convergence in accordance with the second criterion. Two empirical formulae for lift force are considered: one dependent on the slip (relative) velocity of the particle multiplied by the vertical gradient of the horizontal flow velocity component; the other dependent on the difference between the squares of the slip velocity components at the to...

  16. Convergence analysis of a proximal Gauss-Newton method

    CERN Document Server

    Salzo, Saverio

    2011-01-01

    An extension of the Gauss-Newton algorithm is proposed to find local minimizers of penalized nonlinear least squares problems, under generalized Lipschitz assumptions. Convergence results of local type are obtained, as well as an estimate of the radius of the convergence ball. Some applications for solving constrained nonlinear equations are discussed and the numerical performance of the method is assessed on some significant test problems.

  17. Convergent evolution and parallelism in plant domestication revealed by an expanding archaeological record.

    Science.gov (United States)

    Fuller, Dorian Q; Denham, Tim; Arroyo-Kalin, Manuel; Lucas, Leilani; Stevens, Chris J; Qin, Ling; Allaby, Robin G; Purugganan, Michael D

    2014-04-29

    Recent increases in archaeobotanical evidence offer insights into the processes of plant domestication and agricultural origins, which evolved in parallel in several world regions. Many different crop species underwent convergent evolution and acquired domestication syndrome traits. For a growing number of seed crop species, these traits can be quantified by proxy from archaeological evidence, providing measures of the rates of change during domestication. Among domestication traits, nonshattering cereal ears evolved more quickly in general than seed size. Nevertheless, most domestication traits show similarly slow rates of phenotypic change over several centuries to millennia, and these rates were similar across different regions of origin. Crops reproduced vegetatively, including tubers and many fruit trees, are less easily documented in terms of morphological domestication, but multiple lines of evidence outline some patterns in the development of vegecultural systems across the New World and Old World tropics. Pathways to plant domestication can also be compared in terms of the cultural and economic factors occurring at the start of the process. Whereas agricultural societies have tended to converge on higher population densities and sedentism, in some instances cultivation began among sedentary hunter-gatherers whereas more often it was initiated by mobile societies of hunter-gatherers or herder-gatherers.

  18. On the Convergence of the Homotopy Analysis Method for Solving Fredholm Integral Equations

    Directory of Open Access Journals (Sweden)

    Behzad GHANBARI

    2013-07-01

    Full Text Available The aim of this paper is to study the convergence of the Homotopy analysis method (HAM for solving Fredholm integral equations. A sufficient condition for convergence of the method is illustrated. The validity of the presented condition for convergence of the HAM is studied for two examples. The comparison of the obtained results by the method with an exact solution shows that the method is reliable and capable of providing analytic treatment for solving such equations.

  19. Global annealing genetic algorithm and its convergence analysis

    Institute of Scientific and Technical Information of China (English)

    张讲社; 徐宗本; 梁怡

    1997-01-01

    A new selection mechanism termed global annealing selection (GAnS) is proposed for the genetic algorithm. It is proved that the GAnS genetic algorithm converges to the global optimums if and only if the parents are allowed to compete for reproduction, and that the variance of population’s fitness can be used as a natural stopping criterion. Numerical simulations show that the new algorithm has stronger ability to escape from local maximum and converges more rapidly than canonical genetic algorithm.

  20. ASYMPTOTIC ANALYSIS OF SHELLS VIA Γ-CONVERGENCE

    Institute of Scientific and Technical Information of China (English)

    Karine Genevey

    2000-01-01

    We give a new justification of the linear membrane and flexural shell models.We prove that the sequence of scaled energy functionals associated with the scaled problem Γ-converges to the energy functional associated with a two-dimensional model. This two-dimensional model is a membrane or flexural one, depending on the geometric and kinematic conditions. Then, a classical argument allows to give a new proof of the convergence theorems recently obtained by P.G. Ciarlet, V.Lods and B. Miara.

  1. Maximum-entropy clustering algorithm and its global convergence analysis

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    Constructing a batch of differentiable entropy functions touniformly approximate an objective function by means of the maximum-entropy principle, a new clustering algorithm, called maximum-entropy clustering algorithm, is proposed based on optimization theory. This algorithm is a soft generalization of the hard C-means algorithm and possesses global convergence. Its relations with other clustering algorithms are discussed.

  2. Axisymmetric nonconforming element method and its convergence analysis

    Institute of Scientific and Technical Information of China (English)

    陈万吉; 高岩

    1997-01-01

    By virtue of the weighted Sobolev space theory, three convergence tests of the axisymmetric non-conforming element method are established. They consist of the generalized patch test, the F-E-M test and a test which could be used conveniently, called the strong patch test (SPT). In the light of SPT, a class of axisymmetric nonconforming elements is established.

  3. Sequencing the mouse Y chromosome reveals convergent gene acquisition and amplification on both sex chromosomes.

    Science.gov (United States)

    Soh, Y Q Shirleen; Alföldi, Jessica; Pyntikova, Tatyana; Brown, Laura G; Graves, Tina; Minx, Patrick J; Fulton, Robert S; Kremitzki, Colin; Koutseva, Natalia; Mueller, Jacob L; Rozen, Steve; Hughes, Jennifer F; Owens, Elaine; Womack, James E; Murphy, William J; Cao, Qing; de Jong, Pieter; Warren, Wesley C; Wilson, Richard K; Skaletsky, Helen; Page, David C

    2014-11-06

    We sequenced the MSY (male-specific region of the Y chromosome) of the C57BL/6J strain of the laboratory mouse Mus musculus. In contrast to theories that Y chromosomes are heterochromatic and gene poor, the mouse MSY is 99.9% euchromatic and contains about 700 protein-coding genes. Only 2% of the MSY derives from the ancestral autosomes that gave rise to the mammalian sex chromosomes. Instead, all but 45 of the MSY's genes belong to three acquired, massively amplified gene families that have no homologs on primate MSYs but do have acquired, amplified homologs on the mouse X chromosome. The complete mouse MSY sequence brings to light dramatic forces in sex chromosome evolution: lineage-specific convergent acquisition and amplification of X-Y gene families, possibly fueled by antagonism between acquired X-Y homologs. The mouse MSY sequence presents opportunities for experimental studies of a sex-specific chromosome in its entirety, in a genetically tractable model organism.

  4. A note on the convergence of the Zakharov-Kuznetsov equation by homotopy analysis method

    Directory of Open Access Journals (Sweden)

    Amir Fallahzadeh

    2014-07-01

    Full Text Available In this paper, the convergence of Zakharov-Kuznetsov (ZK equation by homotopy analysis method (HAM is investigated. A theorem is proved to guarantee the convergence of HAMand to find the series solution of this equation via a reliable algorithm.

  5. The Power of Inbreeding: NGS-Based GWAS of Rice Reveals Convergent Evolution during Rice Domestication.

    Science.gov (United States)

    Wang, Hongru; Xu, Xun; Vieira, Filipe Garrett; Xiao, Yunhua; Li, Zhikang; Wang, Jun; Nielsen, Rasmus; Chu, Chengcai

    2016-07-01

    Low-coverage whole-genome sequencing is an effective strategy for genome-wide association studies in humans, due to the availability of large reference panels for genotype imputation. However, it is unclear whether this strategy can be utilized in other species without reference panels. Using simulations, we show that this approach is even more relevant in inbred species such as rice (Oryza sativa L.), which are effectively haploid, allowing easy haplotype construction and imputation-based genotype calling, even without the availability of large reference panels. We sequenced 203 rice varieties with well-characterized phenotypes from the United States Department of Agriculture Rice Mini-Core Collection at an average depth of 1.5× and used the data for mapping three traits. For the first two traits, amylose content and seed length, our approach leads to direct identification of the previously identified causal SNPs in the major-effect loci. For the third trait, pericarp color, an important trait underwent selection during domestication, we identified a new major-effect locus. Although known loci can explain color variation in the varieties of two main subspecies of Asian domesticated rice, japonica and indica, the new locus identified is unique to another domesticated rice subgroup, aus, and together with existing loci, can fully explain the major variation in pericarp color in aus. Our discovery of a unique genetic basis of white pericarp in aus provides an example of convergent evolution during rice domestication and suggests that aus may have a domestication history independent of japonica and indica.

  6. A moment-convergence method for stochastic analysis of biochemical reaction networks

    Science.gov (United States)

    Zhang, Jiajun; Nie, Qing; Zhou, Tianshou

    2016-05-01

    Traditional moment-closure methods need to assume that high-order cumulants of a probability distribution approximate to zero. However, this strong assumption is not satisfied for many biochemical reaction networks. Here, we introduce convergent moments (defined in mathematics as the coefficients in the Taylor expansion of the probability-generating function at some point) to overcome this drawback of the moment-closure methods. As such, we develop a new analysis method for stochastic chemical kinetics. This method provides an accurate approximation for the master probability equation (MPE). In particular, the connection between low-order convergent moments and rate constants can be more easily derived in terms of explicit and analytical forms, allowing insights that would be difficult to obtain through direct simulation or manipulation of the MPE. In addition, it provides an accurate and efficient way to compute steady-state or transient probability distribution, avoiding the algorithmic difficulty associated with stiffness of the MPE due to large differences in sizes of rate constants. Applications of the method to several systems reveal nontrivial stochastic mechanisms of gene expression dynamics, e.g., intrinsic fluctuations can induce transient bimodality and amplify transient signals, and slow switching between promoter states can increase fluctuations in spatially heterogeneous signals. The overall approach has broad applications in modeling, analysis, and computation of complex biochemical networks with intrinsic noise.

  7. A moment-convergence method for stochastic analysis of biochemical reaction networks.

    Science.gov (United States)

    Zhang, Jiajun; Nie, Qing; Zhou, Tianshou

    2016-05-21

    Traditional moment-closure methods need to assume that high-order cumulants of a probability distribution approximate to zero. However, this strong assumption is not satisfied for many biochemical reaction networks. Here, we introduce convergent moments (defined in mathematics as the coefficients in the Taylor expansion of the probability-generating function at some point) to overcome this drawback of the moment-closure methods. As such, we develop a new analysis method for stochastic chemical kinetics. This method provides an accurate approximation for the master probability equation (MPE). In particular, the connection between low-order convergent moments and rate constants can be more easily derived in terms of explicit and analytical forms, allowing insights that would be difficult to obtain through direct simulation or manipulation of the MPE. In addition, it provides an accurate and efficient way to compute steady-state or transient probability distribution, avoiding the algorithmic difficulty associated with stiffness of the MPE due to large differences in sizes of rate constants. Applications of the method to several systems reveal nontrivial stochastic mechanisms of gene expression dynamics, e.g., intrinsic fluctuations can induce transient bimodality and amplify transient signals, and slow switching between promoter states can increase fluctuations in spatially heterogeneous signals. The overall approach has broad applications in modeling, analysis, and computation of complex biochemical networks with intrinsic noise.

  8. Stochastic analysis and convergence velocity estimation of genetic algorithms

    Institute of Scientific and Technical Information of China (English)

    郭观七; 喻寿益

    2003-01-01

    Formulizations of mutation and crossover operators independent of representation of solutions are proposed. A kind of precisely quantitative Markov chain of populations of standard genetic algorithms is modeled. It is proved that inadequate parameters of mutation and crossover probabilities degenerate standard genetic algorithm to a class of random search algorithms without selection bias toward any solution based on fitness. After introducing elitist reservation, the stochastic matrix of Markov chain of the best-so-far individual with the highest fitness is derived.The average convergence velocity of genetic algorithms is defined as the mathematical expectation of the mean absorbing time steps that the best-so-far individual transfers from any initial solution to the global optimum. Using the stochastic matrix of the best-so-far individual, a theoretic method and the computing process of estimating the average convergence velocity are proposed.

  9. Convergence analysis of two-node CMFD method for two-group neutron diffusion eigenvalue problem

    Energy Technology Data Exchange (ETDEWEB)

    Jeong, Yongjin; Park, Jinsu [Ulsan National Institute of Science and Technology, UNIST-gil 50, Eonyang-eup, Ulju-gun, Ulsan, 689-798 (Korea, Republic of); Lee, Hyun Chul [Korea Atomic Energy Research Institute, 111 Daedeok-daero 989 beon-gil, Yuseong-gu, Daejeon 305-353 (Korea, Republic of); Lee, Deokjung, E-mail: deokjung@unist.ac.kr [Ulsan National Institute of Science and Technology, UNIST-gil 50, Eonyang-eup, Ulju-gun, Ulsan, 689-798 (Korea, Republic of)

    2015-12-01

    In this paper, the nonlinear coarse-mesh finite difference method with two-node local problem (CMFD2N) is proven to be unconditionally stable for neutron diffusion eigenvalue problems. The explicit current correction factor (CCF) is derived based on the two-node analytic nodal method (ANM2N), and a Fourier stability analysis is applied to the linearized algorithm. It is shown that the analytic convergence rate obtained by the Fourier analysis compares very well with the numerically measured convergence rate. It is also shown that the theoretical convergence rate is only governed by the converged second harmonic buckling and the mesh size. It is also noted that the convergence rate of the CCF of the CMFD2N algorithm is dependent on the mesh size, but not on the total problem size. This is contrary to expectation for eigenvalue problem. The novel points of this paper are the analytical derivation of the convergence rate of the CMFD2N algorithm for eigenvalue problem, and the convergence analysis based on the analytic derivations.

  10. Elucidation of clathrin-mediated endocytosis in tetrahymena reveals an evolutionarily convergent recruitment of dynamin.

    Directory of Open Access Journals (Sweden)

    Nels C Elde

    2005-11-01

    Full Text Available Ciliates, although single-celled organisms, contain numerous subcellular structures and pathways usually associated with metazoans. How this cell biological complexity relates to the evolution of molecular elements is unclear, because features in these cells have been defined mainly at the morphological level. Among these ciliate features are structures resembling clathrin-coated, endocytic pits associated with plasma membrane invaginations called parasomal sacs. The combination of genome-wide sequencing in Tetrahymena thermophila with tools for gene expression and replacement has allowed us to examine this pathway in detail. Here we demonstrate that parasomal sacs are sites of clathrin-dependent endocytosis and that AP-2 localizes to these sites. Unexpectedly, endocytosis in Tetrahymena also involves a protein in the dynamin family, Drp1p (Dynamin-related protein 1. While phylogenetic analysis of AP subunits indicates a primitive origin for clathrin-mediated endocytosis, similar analysis of dynamin-related proteins suggests, strikingly, that the recruitment of dynamin-family proteins to the endocytic pathway occurred independently during the course of the ciliate and metazoan radiations. Consistent with this, our functional analysis suggests that the precise roles of dynamins in endocytosis, as well as the mechanisms of targeting, differ in metazoans and ciliates.

  11. The Convergence Study of the Homotopy Analysis Method for Solving Nonlinear Volterra-Fredholm Integrodifferential Equations

    Directory of Open Access Journals (Sweden)

    Behzad Ghanbari

    2014-01-01

    Full Text Available We aim to study the convergence of the homotopy analysis method (HAM in short for solving special nonlinear Volterra-Fredholm integrodifferential equations. The sufficient condition for the convergence of the method is briefly addressed. Some illustrative examples are also presented to demonstrate the validity and applicability of the technique. Comparison of the obtained results HAM with exact solution shows that the method is reliable and capable of providing analytic treatment for solving such equations.

  12. Ancient mitochondrial DNA reveals convergent evolution of giant short-faced bears (Tremarctinae) in North and South America

    Science.gov (United States)

    Bover, Pere; Soibelzon, Leopoldo; Schubert, Blaine W.; Prevosti, Francisco; Prieto, Alfredo; Martin, Fabiana; Austin, Jeremy J.; Cooper, Alan

    2016-01-01

    The Tremarctinae are a subfamily of bears endemic to the New World, including two of the largest terrestrial mammalian carnivores that have ever lived: the giant, short-faced bears Arctodus simus from North America and Arctotherium angustidens from South America (greater than or equal to 1000 kg). Arctotherium angustidens became extinct during the Early Pleistocene, whereas Arctodus simus went extinct at the very end of the Pleistocene. The only living tremarctine is the spectacled bear (Tremarctos ornatus), a largely herbivorous bear that is today only found in South America. The relationships among the spectacled bears (Tremarctos), South American short-faced bears (Arctotherium) and North American short-faced bears (Arctodus) remain uncertain. In this study, we sequenced a mitochondrial genome from an Arctotherium femur preserved in a Chilean cave. Our molecular phylogenetic analyses revealed that the South American short-faced bears were more closely related to the extant South American spectacled bear than to the North American short-faced bears. This result suggests striking convergent evolution of giant forms in the two groups of short-faced bears (Arctodus and Arctotherium), potentially as an adaptation to dominate competition for megafaunal carcasses. PMID:27095265

  13. Analysis and optimisation of the convergence behaviour of the single channel digital tanlock loop

    Science.gov (United States)

    Al-Kharji Al-Ali, Omar; Anani, Nader; Al-Araji, Saleh; Al-Qutayri, Mahmoud

    2013-09-01

    The mathematical analysis of the convergence behaviour of the first-order single channel digital tanlock loop (SC-DTL) is presented. This article also describes a novel technique that allows controlling the convergence speed of the loop, i.e. the time taken by the phase-error to reach its steady-state value, by using a specialised controller unit. The controller is used to adjust the convergence speed so as to selectively optimise a given performance parameter of the loop. For instance, the controller may be used to speed up the convergence in order to increase the lock range and improve the acquisition speed. However, since increasing the lock range can degrade the noise immunity of the system, in a noisy environment the controller can slow down the convergence speed until locking is achieved. Once the system is in lock, the convergence speed can be increased to improve the acquisition speed. The performance of the SC-DTL system was assessed against similar arctan-based loops and the results demonstrate the success of the controller in optimising the performance of the SC-DTL loop. The results of the system testing using MATLAB/Simulink simulation are presented. A prototype of the proposed system was implemented using a field programmable gate array module and the practical results are in good agreement with those obtained by simulation.

  14. METHODOLOGICAL ASPECTS OF CONTENT ANALYSIS OF CONVERGENCE BETWEEN UKRAINIAN GAAP AND INTERNATIONAL FINANCIAL REPORTING STANDARDS

    Directory of Open Access Journals (Sweden)

    R. Kuzina

    2015-06-01

    Full Text Available The objective conditions of Ukraine’s integration into the global business environment the need to strengthen the accounting and financial re-porting. At the stage of attracting investment in the country there is a need in the preparation of financial statements generally accepted basic prin-ciples of which are based on common international financial reporting standards (IFRS . Relevant is the assessment of convergence of national standards and International Financial Reporting Standards. However, before you conduct content analysis necessary to determine compliance with standards of methodological approaches to the selection of key indicators for the assessment of convergence. The article is to define the methodo-logical approaches to the selection and development of indicators IFRSs list of key elements for further evaluation convergence of national and international standards. To assess the convergence was allocated 187 basic key elements measuring the level of convergence to IFRS. Sampling was carried out based on the professional judgment of the author, the key indicators of the standard, based on the evaluation of the usefulness of accounting information. These figures make it possible to calculate the specific level of convergence of international and national standards and determine how statements prepared by domestic standards corresponding to IFRS. In other words, can with some certainty assert that Ukraine has made (“good practices in IFRS implementation” or not? This calculation will assess the regulatory efforts of government agencies (Ministry of Finance on the approximation of Ukrainian standards and IFRS.

  15. A New Condition for the Uniform Convergence in Fourier Analysis

    Institute of Scientific and Technical Information of China (English)

    周颂平; 乐瑞君

    2004-01-01

    @@ In Fourier analysis, since Fourier coefficients are computable and applicable, people have already established many nice results by assuming monotonicty of the coefficients. One famous classical result is done by Chaundy and Jolliffe[1] as follows:

  16. Convergence and low temperature adaptability analysis of the high temperature series expansion of the free energy.

    Science.gov (United States)

    Zhou, Shiqi

    2013-09-28

    By appealing to the coupling parameter series expansion to calculate the first seven perturbation coefficients of the high temperature series expansion (HTSE) of the free energy, analysis of convergence and low temperature adaptability of the HTSE in calculating fluid thermodynamic properties is performed for the first time; the fluid thermodynamic properties considered include critical parameters, vapor-liquid coexistence curve, thermodynamic characteristic functions, chemical potential, pressure, and constant volume excess heat capacity. To proceed with the analysis, a well known square well model is used as sample; the well widths considered range over a wide interval, and the relevant temperatures amenable to simulation calculations (used as "exact" results to analyze the HTSE) can be both very high and very low. The main discoveries reached are summarized as follows: (1) The HTSE usually converges at the 4th-order truncation, but with decrease of the temperature considered, the lowest truncation order, which makes the HTSE to converge, tends to rise. As a conservative estimate, it is considered that the HTSE always converges for reduced temperature T* higher than 0.25, whereas for T* converges approximately to the correct solution, and the HTSE can be reliably used to calculate the fluid thermodynamic properties, and within this temperature interval, the 4th-order truncation is enough; whereas for T* converge, it does not converge to the correct solution, and the deviations between the HTSE calculations and MC simulations become an ever-prominent issue with the rising of the density, and the slopes of the thermodynamic properties over density are not satisfactorily represented. As a result, the HTSE is not suited for calculations for temperature interval T* < 0.5.

  17. Determining the area of convergence in Bloodstain Pattern Analysis: a probabilistic approach

    CERN Document Server

    Camana, Francesco

    2013-01-01

    A complete procedure for identifying the area of convergence of blood drops originated from a single static source is presented. Both for bloodstains lying on an horizontal and on a vertical plane a complete study is developed, based on error analysis and on an opportunely defined joint probability density for the orientation of the horizontal projections of the trajectories of the drops. The method generates a probabilistic map for the area of convergence, directly linking the angles of impact, and their uncertainties, to the projection on the ground of the point of origin. One of the objectives consists in providing a statistical definition of area of convergence, extending to this topic the mathematical accuracy of the calculation of the angle of impact in bloodstain pattern analysis (BPA).

  18. CONVERGENCE ANALYSIS FOR A NONCONFORMING MEMBRANE ELEMENT ON ANISOTROPIC MESHES

    Institute of Scientific and Technical Information of China (English)

    Dong-yang Shi; Shao-chun Chen; Ichiro Hagiwara

    2005-01-01

    Regular assumption of finite element meshes is a basic condition of most analysis of finite element approximations both for conventional conforming elements and nonconforming elements. The aim of this paper is to present a novel approach of dealing with the approximation of a four-degree nonconforming finite element for the second order elliptic problems on the anisotropic meshes. The optimal error estimates of energy norm and L2-norm without the regular assumption or quasi-uniform assumption are obtained based on some new special features of this element discovered herein. Numerical results are given to demonstrate validity of our theoretical analysis.

  19. Spatial Analysis of Volcanoes at Convergent Margins on Earth

    Science.gov (United States)

    Roberts, R. V.; de Silva, S. L.; Meyers, M.

    2009-12-01

    One of the most obvious patterns seen on the surface of the terrestrial planets is the distribution of volcanoes. On Earth, most volcanoes are distributed in volcanic “arcs” that signal the primary relationship between subduction and volcanism. The distributions of major composite volcanoes in volcanic arcs are thought to reflect the primary magmatic pathways from source to surface. Understanding these patterns therefore may allow fundamental controls on the organization of magmatic plumbing in arcs to be identified. Using a control dataset from the Central Volcanic Zone of the Andes (de Silva and Francis, 1991; Springer-Verlag) we have examined several popular approaches to spatial analysis of volcano distribution in several volcanic arcs (Aleutian, Alaskan, Central American, Northern and Southern volcanic zones of the Andes). Restricting our analysis to major volcanoes of similar age, we find that while clustering is visually obvious in many volcanic arcs it has been rejected as a primary signal by previous analytical efforts (e.g. Bremont d'Ars et al (1995)). We show that the fractal box or grid counting method used previously does not detect clusters and statistical methods such as the Kernel Density Analysis or Single-link Cluster Analysis are better suited for cluster detection. Utilizing both ARC GIS and Matlab to conduct density analyses in combination with statistical software SPlus for the appropriate hypothesis testing methods such as the pooled variance t-test, the Welch Modified two sample t-test, and the f-test we find evidence of clustering in four volcanic arcs whose crustal thickness is greater than or equal to 40 kilometres (Central America, CVZ, NVZ, SVZ). We suggest that clustering is the surface manifestation of upper crustal diffusion of primary magmatic pathways, which in other places manifests as a single volcano. The inter-cluster distance is a thus reflection of primary magmatic pathways and thus equivalent to inter-volcano distance

  20. Spectrum analysis of hydrogen plasma in spherically convergent beam fusion

    Energy Technology Data Exchange (ETDEWEB)

    Ogasawara, Kazuki; Yamauchi, Kunihito; Watanabe, Masato; Sunaga, Yoshitaka; Hotta, Eiki [Tokyo Institute of Technology, Dept. of Energy Sciences, Yokohama, Kanagawa (Japan); Okino, Akitoshi [Tokyo Institute of Technology, Dept. of Electrical and Electronic Engineering, Tokyo (Japan)

    2001-09-01

    Spectroscopic analysis of spherical glow discharge fusion device was carried out using hydrogen gas. Effects of the discharge current and cathode voltage on spectrum profiles of hydrogen Balmar lines were measured. The profiles of all hydrogen lines were broadened with the cathode voltage. From the relationship between the maximum broadening width and the cathode voltage, it was indicated that the broadening was caused by the Doppler effect. From the spatial distribution of emission intensity, it was found that plasma core size became larger with discharge current and smaller with cathode voltage. (author)

  1. Convergence Time Analysis of Particle Swarm Optimization Based on Particle Interaction

    Directory of Open Access Journals (Sweden)

    Chao-Hong Chen

    2011-01-01

    Full Text Available We analyze the convergence time of particle swarm optimization (PSO on the facet of particle interaction. We firstly introduce a statistical interpretation of social-only PSO in order to capture the essence of particle interaction, which is one of the key mechanisms of PSO. We then use the statistical model to obtain theoretical results on the convergence time. Since the theoretical analysis is conducted on the social-only model of PSO, instead of on common models in practice, to verify the validity of our results, numerical experiments are executed on benchmark functions with a regular PSO program.

  2. A convergence analysis for a sweeping preconditioner for block tridiagonal systems of linear equations

    KAUST Repository

    Bagci, Hakan

    2014-11-11

    We study sweeping preconditioners for symmetric and positive definite block tridiagonal systems of linear equations. The algorithm provides an approximate inverse that can be used directly or in a preconditioned iterative scheme. These algorithms are based on replacing the Schur complements appearing in a block Gaussian elimination direct solve by hierarchical matrix approximations with reduced off-diagonal ranks. This involves developing low rank hierarchical approximations to inverses. We first provide a convergence analysis for the algorithm for reduced rank hierarchical inverse approximation. These results are then used to prove convergence and preconditioning estimates for the resulting sweeping preconditioner.

  3. Canonical Least-Squares Monte Carlo Valuation of American Options: Convergence and Empirical Pricing Analysis

    Directory of Open Access Journals (Sweden)

    Xisheng Yu

    2014-01-01

    Full Text Available The paper by Liu (2010 introduces a method termed the canonical least-squares Monte Carlo (CLM which combines a martingale-constrained entropy model and a least-squares Monte Carlo algorithm to price American options. In this paper, we first provide the convergence results of CLM and numerically examine the convergence properties. Then, the comparative analysis is empirically conducted using a large sample of the S&P 100 Index (OEX puts and IBM puts. The results on the convergence show that choosing the shifted Legendre polynomials with four regressors is more appropriate considering the pricing accuracy and the computational cost. With this choice, CLM method is empirically demonstrated to be superior to the benchmark methods of binominal tree and finite difference with historical volatilities.

  4. Convergence Analysis of Saddle Point Problems in Time Varying Wireless Systems - Control Theoretical Approach

    CERN Document Server

    Chen, Junting

    2011-01-01

    Saddle point problems arise from many wireless applications, and primal-dual iterative algorithms are widely applied to find the saddle points. In the existing literature, the convergence results of such algorithms are established assuming the problem specific parameters remain unchanged during the iterations. However, this assumption is unrealistic in time varying wireless systems, as explicit message passing is usually involved in the iterations and the channel state information (CSI) may change in a time scale comparable to the algorithm update period. This paper investigates the convergence behavior and the tracking error of primal-dual iterative algorithms under time varying CSI. The convergence results are established by studying the stability of an equivalent virtual dynamic system derived in the paper, and the Lyapunov theory is applied for the stability analysis. We show that the average tracking error is proportional to the time variation rate of the CSI. Based on these analyses, we also derive an a...

  5. Maximum-entropy clustering algorithm and its global convergence analysis

    Institute of Scientific and Technical Information of China (English)

    ZHANG; Zhihua

    2001-01-01

    [1]Bezdek, J. C., Pattern Recognition with Fuzzy Objective Function Algorithm. New York: Plenum, 1981.[2]Krishnapuram, R., Keller, J., A possibilistic approach to clustering, IEEE Trans. on Fuzzy Systems, 1993, 1(2): 98.[3]Yair, E., Zeger, K., Gersho, A., Competitive learning and soft competition for vector quantizer design, IEEE Trans on Signal Processing, 1992, 40(2): 294.[4]Pal, N. R., Bezdek, J. C., Tsao, E. C. K., Generalized clustering networks and Kohonen's self-organizing scheme, IEEE Trans on Neural Networks, 1993, 4(4): 549.[5]Karayiannis, N. B., Bezdek, J. C., Pal, N. R. et al., Repair to GLVQ: a new family of competitive learning schemes, IEEE Trans on Neural Networks, 1996, 7(5): 1062.[6]Karayiannis, N. B., Pai, P. I., Fuzzy algorithms for learning vector quantization, IEEE Trans. on Neural Networks, 1996, 7(5): 1196.[7]Karayiannis, N. B., A methodology for constructing fuzzy algorithms for learning vector quantization, IEEE Trans. on Neural Networks, 1997, 8(3): 505.[8]Karayiannis, N. B., Bezdek, J. C., An integrated approach to fuzzy learning vector quantization and fuzzy C-Means clustering, IEEE Trans. on Fuzzy Systems, 1997, 5(4): 622.[9]Li Xing-si, An efficient approach to nonlinear minimax problems, Chinese Science Bulletin? 1992, 37(10): 802.[10]Li Xing-si, An efficient approach to a class of non-smooth optimization problems, Science in China, Series A,1994, 37(3): 323.[11]. Zangwill, W., Non-linear Programming: A Unified Approach, Englewood Cliffs: Prentice-Hall, 1969.[12]. Fletcher, R., Practical Methods of Optimization,2nd ed., New York: John Wiley & Sons, 1987.[13]. Zhang Zhihua, Zheng Nanning, Wang Tianshu, Behavioral analysis and improving of generalized LVQ neural network, Acta Automatica Sinica, 1999, 25(5): 582.[14]. Kirkpatrick, S., Gelatt, C. D., Vecchi, M. P., Optimization by simulated annealing, Science, 1983, 220(3): 671.[15]. Ross, K., Deterministic annealing for

  6. Integrative network analysis unveils convergent molecular pathways in Parkinson's disease and diabetes.

    Directory of Open Access Journals (Sweden)

    Jose A Santiago

    Full Text Available BACKGROUND: Shared dysregulated pathways may contribute to Parkinson's disease and type 2 diabetes, chronic diseases that afflict millions of people worldwide. Despite the evidence provided by epidemiological and gene profiling studies, the molecular and functional networks implicated in both diseases, have not been fully explored. In this study, we used an integrated network approach to investigate the extent to which Parkinson's disease and type 2 diabetes are linked at the molecular level. METHODS AND FINDINGS: Using a random walk algorithm within the human functional linkage network we identified a molecular cluster of 478 neighboring genes closely associated with confirmed Parkinson's disease and type 2 diabetes genes. Biological and functional analysis identified the protein serine-threonine kinase activity, MAPK cascade, activation of the immune response, and insulin receptor and lipid signaling as convergent pathways. Integration of results from microarrays studies identified a blood signature comprising seven genes whose expression is dysregulated in Parkinson's disease and type 2 diabetes. Among this group of genes, is the amyloid precursor protein (APP, previously associated with neurodegeneration and insulin regulation. Quantification of RNA from whole blood of 192 samples from two independent clinical trials, the Harvard Biomarker Study (HBS and the Prognostic Biomarker Study (PROBE, revealed that expression of APP is significantly upregulated in Parkinson's disease patients compared to healthy controls. Assessment of biomarker performance revealed that expression of APP could distinguish Parkinson's disease from healthy individuals with a diagnostic accuracy of 80% in both cohorts of patients. CONCLUSIONS: These results provide the first evidence that Parkinson's disease and diabetes are strongly linked at the molecular level and that shared molecular networks provide an additional source for identifying highly sensitive biomarkers

  7. Sustainable development induction in organizations: a convergence analysis of ISO standards management tools' parameters.

    Science.gov (United States)

    Merlin, Fabrício Kurman; Pereira, Vera Lúciaduarte do Valle; Pacheco, Waldemar

    2012-01-01

    Organizations are part of an environment in which they are pressured to meet society's demands and acting in a sustainable way. In an attempt to meet such demands, organizations make use of various management tools, among which, ISO standards are used. Although there are evidences of contributions provided by these standards, it is questionable whether its parameters converge for a possible induction for sustainable development in organizations. This work presents a theoretical study, designed on structuralism world view, descriptive and deductive method, which aims to analyze the convergence of management tools' parameters in ISO standards. In order to support the analysis, a generic framework for possible convergence was developed, based on systems approach, linking five ISO standards (ISO 9001, ISO 14001, OHSAS 18001, ISO 31000 and ISO 26000) with sustainable development and positioning them according to organization levels (strategic, tactical and operational). The structure was designed based on Brundtland report concept. The analysis was performed exploring the generic framework for possible convergence based on Nadler and Tushman model. The results found the standards can contribute to a possible sustainable development induction in organizations, as long as they meet certain minimum conditions related to its strategic alignment.

  8. Regional Convergence and Sustainable Development in China

    Directory of Open Access Journals (Sweden)

    Fang Yang

    2016-01-01

    Full Text Available Based on the convergence theory of economic growth, this paper extends this concept to the human development index and carries out an empirical analysis of regional development in China between 1997 and 2006. Our research shows that the conditional convergence has been identified. Investment in fixed assets, government expenditure on education, health and infrastructure construction have positive effects on regional convergence of social development. Population weighted analysis of human development index provides support for weak convergence amongst provinces. Analysis of dynamics of regional distribution reveals the club convergence, which indicate two different convergence states. Central China is in the shade and lags behind, giving rise to the so-called “central downfall”. To solve this problem, the “Rise of Central China” Plan is necessary to promote the connection between coastal and inland regions of China and reduce the regional development gap.

  9. Mean-square convergence analysis of ADALINE training with minimum error entropy criterion.

    Science.gov (United States)

    Chen, Badong; Zhu, Yu; Hu, Jinchun

    2010-07-01

    Recently, the minimum error entropy (MEE) criterion has been used as an information theoretic alternative to traditional mean-square error criterion in supervised learning systems. MEE yields nonquadratic, nonconvex performance surface even for adaptive linear neuron (ADALINE) training, which complicates the theoretical analysis of the method. In this paper, we develop a unified approach for mean-square convergence analysis for ADALINE training under MEE criterion. The weight update equation is formulated in the form of block-data. Based on a block version of energy conservation relation, and under several assumptions, we carry out the mean-square convergence analysis of this class of adaptation algorithm, including mean-square stability, mean-square evolution (transient behavior) and the mean-square steady-state performance. Simulation experimental results agree with the theoretical predictions very well.

  10. Convergence analysis of the alternating RGLS algorithm for the identification of the reduced complexity Volterra model.

    Science.gov (United States)

    Laamiri, Imen; Khouaja, Anis; Messaoud, Hassani

    2015-03-01

    In this paper we provide a convergence analysis of the alternating RGLS (Recursive Generalized Least Square) algorithm used for the identification of the reduced complexity Volterra model describing stochastic non-linear systems. The reduced Volterra model used is the 3rd order SVD-PARAFC-Volterra model provided using the Singular Value Decomposition (SVD) and the Parallel Factor (PARAFAC) tensor decomposition of the quadratic and the cubic kernels respectively of the classical Volterra model. The Alternating RGLS (ARGLS) algorithm consists on the execution of the classical RGLS algorithm in alternating way. The ARGLS convergence was proved using the Ordinary Differential Equation (ODE) method. It is noted that the algorithm convergence canno׳t be ensured when the disturbance acting on the system to be identified has specific features. The ARGLS algorithm is tested in simulations on a numerical example by satisfying the determined convergence conditions. To raise the elegies of the proposed algorithm, we proceed to its comparison with the classical Alternating Recursive Least Squares (ARLS) presented in the literature. The comparison has been built on a non-linear satellite channel and a benchmark system CSTR (Continuous Stirred Tank Reactor). Moreover the efficiency of the proposed identification approach is proved on an experimental Communicating Two Tank system (CTTS).

  11. Diffusion Patterns in Convergence among High-Technology Industries: A Co-Occurrence-Based Analysis of Newspaper Article Data

    Directory of Open Access Journals (Sweden)

    Hyeokseong Lee

    2016-10-01

    Full Text Available Firms in high-technology industries have faced great technological and market uncertainty and volatility in the past few decades. In order to be competitive and sustainable in this environment, firms have been pursuing technological innovation, product differentiation, vertical integration, and alliances, which eventually drive industry convergence, defined as the process of blurring boundaries between previously distinct industries. Although industry convergence has greatly affected industrial structure and the economy, little research has investigated this phenomenon, especially its diffusion patterns; thus, it is still unclear which industries are converging more rapidly or have a higher potential for convergence. This paper explores these issues by investigating industry convergence in U.S. high-technology industries, using a large set of newspaper articles from 1987 to 2012. We perform a co-occurrence-based analysis to obtain information on industry convergence and estimate its diffusion patterns using an internal-influence logistic model. We find heterogeneous diffusion patterns, depending on convergent-industry pairs and their wide dispersion. In addition, we find that the potential degree of industry convergence is significantly negatively associated with its growth rate, which indicates that a great deal of time will be required for industry convergence between high-technology industries with this high potential to achieve a high degree of convergence.

  12. Computational Fluid Dynamics (CFD Analysis of Natural Convection of Convergent-Divergent Fins in Marine Environments

    Directory of Open Access Journals (Sweden)

    K. Alawadhi

    2014-12-01

    Full Text Available Computational Fluid Dynamics (CFD analysis was carried out for the convergent-divergent fins arranged inline and staggered on the base plate as per the experimental setup provided in the technical paper [1]. This paper reports on the validation of results of modeling and simulation in CFD. The simulation was carried out using the ANSYS 12.0 as the CFD modeling software. The main objective of the CFD analysis was to calculate the temperature distribution on the surface of the base plate and surface of the convergent-divergent fins for the given inline and staggered arrangement of fins due to the effect of natural convection heat transfer for different heat power inputs, and also to compare the CFD results with the experimental results.

  13. On the convergence and causality of a frequency domain method for dynamic structural analysis

    Institute of Scientific and Technical Information of China (English)

    Kuifu Chen; Senwen Zhang

    2006-01-01

    Venanico-Filho et al.developed an elegant matrix formulation for dynamic analysis by frequency domain (FD),but the convergence,causality and extended period need further refining.In the present Paper,it was argued that:(1) under reasonable assumptions (approximating the frequency response function by the discrete Fourier transform of the discretized unitary impulse response function),the matrix formulation by FD is equivalent to a circular convolution;(2) to avoid the wraparound Interference,the excitation vector and impulse response must be padded with enough zeros:(3) provided that the zero padding requirement satisfied,the convergence and accuracy of direct time domain analysis,which is equivalent to that by FD,are guaranteed by the numerical integration scheme;(4) the imaginary part of the computational response approaching zero is due to the continuity of the impulse response functions.

  14. Differences and similarities in the Cocos-North America and Cocos-Caribbean convergence, as revealed by seismic moment tensors

    Science.gov (United States)

    Guzmán-Speziale, Marco; Zúñiga, F. Ramón

    2016-11-01

    We investigate the differences and similarities in Cocos-North America and Cocos-Caribbean convergence, reflected by seismicity and seismic moment. We use well-located hypocenters of earthquakes in the convergence margin, as well as within the subducted slab. We sort these data by number of events in the two converging margins, and by their magnitudes. We also use this database to determine an improved geometric model of the subducted slab. We find a shallow-dipping subducting Cocos plate underneath North America and a steeper dip slab under the Caribbean plate. The transition between them appears to be smooth. Centroid Moment tensor solutions indicate that almost all of the thrust-faulting earthquakes along Cocos-North America take place at shallow depths. Normal-faulting events along this margin only take place to depths of 100 km. Thrust- and normal-faulting events take place at all depths along the Cocos-Caribbean margin. Cummulative scalar seismic moment for shallow, thrust-faulting events, is larger along Cocos-North America. T axes of intermediate-depth, normal- and thrust-faulting events show that the subducted Cocos plate is in maximum tension along the direction of maximum dip. Azimuth of earthquake slip vectors for shallow events along the Cocos-North America margin agree well with the direction of plate convergence. They do not agree along the Cocos-Caribbean margin; instead, agreement is found with Cocos-North America relative plate motion. Compensated Linear Vector Dipole (CLVD) ratio, which measures how different a seismic source is from a pure double-couple, along both margins is inversely proportional to scalar seismic moment, indicating that for larger magnitudes rupture is closer to a double-couple mechanism than at smaller moments.

  15. Uniform Convergence Analysis for Singularly Perturbed Elliptic Problems with Parabolic Layers

    Institute of Scientific and Technical Information of China (English)

    Jichun Li; Yitung Chen

    2008-01-01

    In this paper, using Lin's integral identity technique, we prove the optimal uniform convergence O(Nx-2 ln2Nx+ Ny-2 In2Ny) in the L2-norm for singularly per-turbed problems with parabolic layers. The error estimate is achieved by bilinear fi-nite elements on a Shishkin type mesh. Here Nx and Ny are the number of elements in the x- and y-directions, respectively. Numerical results are provided supporting our theoretical analysis.

  16. Convergence analysis of a class of massively parallel direction splitting algorithms for the Navier-Stokes equations in simple domains

    KAUST Repository

    Guermond, Jean-Luc

    2012-01-01

    We provide a convergence analysis for a new fractional timestepping technique for the incompressible Navier-Stokes equations based on direction splitting. This new technique is of linear complexity, unconditionally stable and convergent, and suitable for massive parallelization. © 2012 American Mathematical Society.

  17. IT-BT convergence technology

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2012-12-15

    This book explains IT-BT convergence technology as the future technology, which includes a prolog, easy IT-BT convergence technology that has infinite potentials for new value, policy of IT-BT convergence technology showing the potential of smart Korea, IT-BT convergence opening happy future, for the new future of IT powerful nation Korea with IT-BT convergence technology and an epilogue. This book reveals the conception, policy, performance and future of IT-BT convergence technology.

  18. Diclosure and transparency in public sector: an analysis of convergence of the principles of governance

    Directory of Open Access Journals (Sweden)

    Luzia Zorzal

    2015-09-01

    Full Text Available Introduction: Studies on disclosure of private institutions are common, but do not occur when it comes to public institutions, where the disclosure of management is still very limited. Purpose: The article is part of doctorate research in Information Science in progress and investigates the principles of disclosure and transparency in the light of good governance practices applied to the public sector, to reduce information asymmetry and presents part of this research. Methodology: The methodological procedures were performed literature search and content analysis to identify the principles and standards of good governance practices recommended for public administration, aiming to systematize these recommendations as instruments of governance and verify the convergence of the principles of disclosure and transparency. Results: Partial results show convergence of disclosure and transparency principles. Conclusions: Indicate that public institutions should worry about performing the practices of good governance as a way to mitigate the informational asymmetry.

  19. Analysis of convergence for initial condition estimation of coupled map lattices based on symbolic dynamics

    Institute of Scientific and Technical Information of China (English)

    Sun Li-Sha; Kang Xiao-Yun; Lin Lan-Xin

    2010-01-01

    A novel approach to the inverse problem of diffusively coupled map lattices is systematically investigated by utilizing the symbolic vector dynamics. The relationship between the performance of initial condition estimation and the structural feature of dynamical system is proved theoretically. It is found that any point in a spatiotemporal coupled system is not necessary to converge to its initial value with respect to sufficient backward iteration, which is directly relevant to the coupling strength and local mapping function. When the convergence is met, the error bound in estimating the initial condition is proposed in a noiseless environment, which is determined by the dimension of attractors and metric entropy of the system. Simulation results further confirm the theoretic analysis, and prove that the presented method provides the important theory and experimental results for better analysing and characterizing the spatiotemporal complex behaviours in an actual system.

  20. Aspects of incidence of convergence telejournalism: analysis of fragments of context instances Brazilian

    Directory of Open Access Journals (Sweden)

    Eloisa Joseane da Cunha Klein

    2012-12-01

    Full Text Available This text reflects on the affectations of mediatization on the field of journalism in the context of digital convergence, considering changes in news production, shift in the relationship of the actors in the field and with the reception. The text makes preliminary considerations on the ways in which television journalism interacts with digital tools - from partial analysis of how television program Profession Reporter uses such tools. Also stressed are two cases on the affectation of the social uses of digital media on the television news: the first, linked to the impact of a Profession Reporter edition on domestic violence (2009, the second, the impact of a case highlighted in social networks in TV journalism. Thus, we consider the issues of communication circulation, accentuated by digital convergence

  1. The Analysis of the Real Convergence of the Countries from Central and Eastern Europe

    Directory of Open Access Journals (Sweden)

    Roxana Badircea

    2016-11-01

    Full Text Available This thesis treats extremely present aspects regarding the evolutions of the emerging economies within the new member states of the European Union insisting on the analysis of the convergence process from a real perspective. Beside the achievement of a monetary union, one of the fundamental objectives of the European Union is represented by the reducing of the disparities regarding the level of development among the member states. One of the ways of appreciating the reduction of the disparities between the economies involve a reduction of the gap as far as the GDP level/inhabitant is concerned or in other words, a real convergence. A series of statistic data are analysed in order to point out the extent to which the central ad East-European states have managed to reduce the gap in report to the developed member states of the EMU, using indicators for the appreciation of the real convergence: the GDP per inhabitant, the monthly average salary, the poverty rate, the contribution of the main sectors of the economy in the formation of the GDP and the unemployment rate. From the analysis of the statistic data one can observe that the highest degree of real convergence is held by Slovenia, which distanced a lot from the other EEC states, followed by the Czech Republic. Regarding from the point of view of the evolution of the EEC countries during the entire analysed period, based on the dynamics of the indicators and of the speed of catching up the gaps we can also notice the performance of the Baltic countries. Unfortunately, Romania and Bulgaria are way behind the other EEC countries.

  2. PHYSALIS: a new method for particle flow simulation. Part III: convergence analysis of two-dimensional flows

    Science.gov (United States)

    Huang, Huaxiong; Takagi, Shu

    2003-08-01

    In this paper, we study the convergence property of PHYSALIS when it is applied to incompressible particle flows in two-dimensional space. PHYSALIS is a recently proposed iterative method which computes the solution without imposing the boundary conditions on the particle surfaces directly. Instead, a consistency equation based on the local (near particle) representation of the solution is used as the boundary conditions. One of the important issues needs to be addressed is the convergence properties of the iterative procedure. In this paper, we present the convergence analysis using Laplace and biharmonic equations as two model problems. It is shown that convergence of the method can be achieved but the rate of convergence depends on the relative locations of the cages. The results are directly related to potential and Stokes flows. However, they are also relevant to Navier-Stokes flows, heat conduction in composite media, and other problems.

  3. Comparative analysis of the quality of European institutions 2003-2009: convergence or divergence?

    Directory of Open Access Journals (Sweden)

    Krešimir Jurlin

    2010-03-01

    Full Text Available This paper gives a comparative cross-country analysis of the quality of the institutional framework that underpins economic governance in European countries. The paper attempts to identify the trends of change in the quality of institutions and determine if there is a convergence in the quality of EU institutions. The countries included in the analysis are selected groups of EU countries and the Western Balkan Countries. The analysis is based on the results of the executive officers’ opinion surveys conducted by the World Economic Forum for the Global Competitiveness Index 2003-2009. In order to identify the relative quality of public institutions and the trend towards convergence/divergence for different clusters of EU countries, a three-pillar composite indicator of institutional quality was constructed from available WEF indicators. The analysis was conducted at the aggregated level as well as for individual countries and pillars, measured in terms of difference to an average rank of survey response in the EU-14 + EU-8 members.

  4. Stability Analysis of A Neuro-Identification Scheme with Asymptotic Convergence

    Directory of Open Access Journals (Sweden)

    José A. R. Vargas

    2012-07-01

    Full Text Available This paper focuses on the stability and convergence analysis of a neuro-identification scheme for uncertain nonlinear systems. Based on linearly parameterized neural networks and the previous knowledge of upper bounds for the approximation error and disturbances, a robust modification of the descent gradient algorithm is proposed to make the overall identification process stable, and, in addition, the on-line residual prediction error asymptotically null, despite the presence of approximation error and disturbances. A simulation study to show the application and comparative performance of the proposed algorithm is presented.

  5. ON SPECTRAL METHODS FOR VOLTERRA INTEGRAL EQUATIONS AND THE CONVERGENCE ANALYSIS

    Institute of Scientific and Technical Information of China (English)

    Tao Tang; Xiang Xu; Jin Cheng

    2008-01-01

    The main purpose of this work is to provide a novel numerical approach for the Volterra integral equations based on a spectral approach. A Legendre-collocation method is pro-posed to solve the Volterra integral equations of the second kind. We provide a rigorous error analysis for the proposed method, which indicates that the numerical errors decay exponentially provided that the kernel function and the source function are sufficiently smooth. Numerical results confirm the theoretical prediction of the exponential rate of convergence. The result in this work seems to be the first successful spectral approach (with theoretical justification) for the Volterra type equations.

  6. Hybrid Bernstein Block-Pulse Functions Method for Second Kind Integral Equations with Convergence Analysis

    Directory of Open Access Journals (Sweden)

    Mohsen Alipour

    2014-01-01

    Full Text Available We introduce a new combination of Bernstein polynomials (BPs and Block-Pulse functions (BPFs on the interval [0, 1]. These functions are suitable for finding an approximate solution of the second kind integral equation. We call this method Hybrid Bernstein Block-Pulse Functions Method (HBBPFM. This method is very simple such that an integral equation is reduced to a system of linear equations. On the other hand, convergence analysis for this method is discussed. The method is computationally very simple and attractive so that numerical examples illustrate the efficiency and accuracy of this method.

  7. Convergence analysis for general linear methods applied to stiff delay differential equations

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    For Runge-Kutta methods applied to stiff delay differential equations (DDEs), the concept of D-convergence was proposed, which is an extension to that of B-convergence in ordinary differential equations (ODEs). In this paper, D-convergence of general linear methods is discussed and the previous related results are improved. Some order results to determine D-convergence of the methods are obtained.

  8. Convergence analysis of variational and non-variational multigrid algorithms for the Laplace-Beltrami operator

    KAUST Repository

    Bonito, Andrea

    2012-09-01

    We design and analyze variational and non-variational multigrid algorithms for the Laplace-Beltrami operator on a smooth and closed surface. In both cases, a uniform convergence for the V -cycle algorithm is obtained provided the surface geometry is captured well enough by the coarsest grid. The main argument hinges on a perturbation analysis from an auxiliary variational algorithm defined directly on the smooth surface. In addition, the vanishing mean value constraint is imposed on each level, thereby avoiding singular quadratic forms without adding additional computational cost. Numerical results supporting our analysis are reported. In particular, the algorithms perform well even when applied to surfaces with a large aspect ratio. © 2011 American Mathematical Society.

  9. Searching for convergent evolution in manganese superoxidase dismutase using hydrophobic cluster analysis

    Directory of Open Access Journals (Sweden)

    Heng Xiang

    2014-06-01

    Full Text Available There are numerous examples of convergent evolution in nature. Major ecological adaptations such as flight, loss of limbs in vertebrates, pesticide resistance, adaptation to a parasitic way of life, etc., have all evolved more than once, as seen by their analogous functions in separate taxa. But what about protein evolution? Does the environment have a strong enough influence on intracellular processes that enzymes and other functional proteins play, to evolve similar functional roles separately in different organisms? Manganese Superoxide Dismutase (MnSOD is a manganesedependant metallo-enzyme which plays a crucial role in protecting cells from anti-oxidative stress by eliminating reactive (superoxide oxygen species. It is a ubiquitous housekeeping enzyme found in nearly all organisms. In this study we compare phylogenies based on MnSOD protein sequences to those based on scores from Hydrophobic Cluster Analysis (HCA. We calculated HCA similarity values for each pair of taxa to obtain a pair-wise distance matrix. A UPGMA tree based on the HCA distance matrix and a common tree based on the primary protein sequence for MnSOD was constructed. Differences between these two trees within animals, enterobacteriaceae, planctomycetes and cyanobacteria are presented and cited as possible examples of convergence. We note that several residue changes result in changes in hydrophobicity at positions which apparently are under the effect of positive selection.

  10. Food Price Inflation Rates in the Euro Zone: Distribution Dynamics and Convergence Analysis

    Directory of Open Access Journals (Sweden)

    Angelos Liontakis

    2012-01-01

    Full Text Available It is widely recognized that inflation as a monetary phenomenon is determined by money supply changes. In the short run, however, several factors may lead to inflation rate differentials among different regions in the same country or among different countries in a monetary union. This paper examines the mean reversion attitude of food price inflation rates in the Euro zone, borrowing the concepts and developments from the recent growth literature and using panel unit root tests. Additionally, in order to capture sufficiently the evolving distributional dynamics, nonparametric econometric methods are also implemented. Finally, the comovement of the inflation rates among different food subgroups is also explored. The data consist of monthly observations of the EU harmonized consumer price indices of food and three different food subgroups (meat, bread and cereals, and vegetables for the 12 older member states of the Euro zone, covering the period from 1997 to 2010. The results do not fully support the hypothesis of the food price inflation rates convergence for the whole period under investigation. Mean reversion shows up in different time periods and in different food categories. Moreover, the analysis of distribution dynamics sheds light to different aspects of convergence and highlights processes like club formation and polarization.

  11. Structural analysis of converging jets in a triple torch plasma system

    CERN Document Server

    Ramachandran, K

    2003-01-01

    A three-dimensional numerical model is developed to clarify the structure of the converging jets in a triple torch plasma system. Three individual argon plasma jets, issued into atmospheric argon, are mixed with given angle of convergence and form a converged plasma jet. Predicted results show that thermo-fluid fields of the converging plasma jets are symmetric with three symmetric sections at an interval 120 deg. Symmetry and uniformity of thermo-fluid fields increase with decreasing angle of convergence. Temperature field is more sensitive to angle of convergence than the velocity field. Symmetry of thermo-fluid fields is improved in downstream direction. A dip in the velocity fields corresponds to poor mixing and diffusion of velocity fields of three individual plasma jets. Central gas injection decreases converging jets temperature significantly.

  12. Convergence analysis of particle swarm optimization (PSO) method on the with-in host dengue infection treatment model

    Science.gov (United States)

    Handayani, D.; Nuraini, N.; Tse, O.; Saragih, R.; Naiborhu, J.

    2016-04-01

    PSO is a computational optimization method motivated by the social behavior of organisms like bird flocking, fish schooling and human social relations. PSO is one of the most important swarm intelligence algorithms. In this study, we analyze the convergence of PSO when it is applied to with-in host dengue infection treatment model simulation in our early research. We used PSO method to construct the initial adjoin equation and to solve a control problem. Its properties of control input on the continuity of objective function and ability of adapting to the dynamic environment made us have to analyze the convergence of PSO. With the convergence analysis of PSO we will have some parameters that ensure the convergence result of numerical simulations on this model using PSO.

  13. Convergence and error estimation in free energy calculations using the weighted histogram analysis method.

    Science.gov (United States)

    Zhu, Fangqiang; Hummer, Gerhard

    2012-02-05

    The weighted histogram analysis method (WHAM) has become the standard technique for the analysis of umbrella sampling simulations. In this article, we address the challenges (1) of obtaining fast and accurate solutions of the coupled nonlinear WHAM equations, (2) of quantifying the statistical errors of the resulting free energies, (3) of diagnosing possible systematic errors, and (4) of optimally allocating of the computational resources. Traditionally, the WHAM equations are solved by a fixed-point direct iteration method, despite poor convergence and possible numerical inaccuracies in the solutions. Here, we instead solve the mathematically equivalent problem of maximizing a target likelihood function, by using superlinear numerical optimization algorithms with a significantly faster convergence rate. To estimate the statistical errors in one-dimensional free energy profiles obtained from WHAM, we note that for densely spaced umbrella windows with harmonic biasing potentials, the WHAM free energy profile can be approximated by a coarse-grained free energy obtained by integrating the mean restraining forces. The statistical errors of the coarse-grained free energies can be estimated straightforwardly and then used for the WHAM results. A generalization to multidimensional WHAM is described. We also propose two simple statistical criteria to test the consistency between the histograms of adjacent umbrella windows, which help identify inadequate sampling and hysteresis in the degrees of freedom orthogonal to the reaction coordinate. Together, the estimates of the statistical errors and the diagnostics of inconsistencies in the potentials of mean force provide a basis for the efficient allocation of computational resources in free energy simulations.

  14. A genotype network reveals homoplastic cycles of convergent evolution in influenza A (H3N2) haemagglutinin

    OpenAIRE

    Wagner,Andreas

    2014-01-01

    Networks of evolving genotypes can be constructed from the worldwide time-resolved genotyping of pathogens like influenza viruses. Such genotype networks are graphs where neighbouring vertices (viral strains) differ in a single nucleotide or amino acid. A rich trove of network analysis methods can help understand the evolutionary dynamics reflected in the structure of these networks. Here, I analyse a genotype network comprising hundreds of influenza A (H3N2) haemagglutinin genes. The network...

  15. Analysis of the diversity of population and convergence of genetic algorithms based on Negentropy

    Institute of Scientific and Technical Information of China (English)

    Zhang Lianying; Wang Anmin

    2005-01-01

    With its wide use in different fields, the problem of the convergence of simple genetic algorithms (GAs) has been concerned. In the past, the research on the convergence of GAs was based on Holland' s model theorem. The diversity of the evolutionary population and the convergence of GAs are studied by using the concept of negentropy based on the discussion of the characteristic of GA. Some test functions are used to test the convergence of GAs, and good results have been obtained. It is shown that the global optimization may be obtained by selecting appropriate parameters of simple GAs if the evolution time is enough.

  16. Convergent evidence from multimodal imaging reveals amygdala abnormalities in schizophrenic patients and their first-degree relatives.

    Directory of Open Access Journals (Sweden)

    Lin Tian

    Full Text Available BACKGROUND: Shared neuropathological features between schizophrenic patients and their first-degree relatives have potential as indicators of genetic vulnerability to schizophrenia. We sought to explore genetic influences on brain morphology and function in schizophrenic patients and their relatives. METHODS: Using a multimodal imaging strategy, we studied 33 schizophrenic patients, 55 of their unaffected parents, 30 healthy controls for patients, and 29 healthy controls for parents with voxel-based morphometry of structural MRI scans and functional connectivity analysis of resting-state functional MRI data. RESULTS: Schizophrenic patients showed widespread gray matter reductions in the bilateral frontal cortices, bilateral insulae, bilateral occipital cortices, left amygdala and right thalamus, whereas their parents showed more localized reductions in the left amygdala, left thalamus and right orbitofrontal cortex. Patients and their parents shared gray matter loss in the left amygdala. Further investigation of the resting-state functional connectivity of the amygdala in the patients showed abnormal functional connectivity with the bilateral orbitofrontal cortices, bilateral precunei, bilateral dorsolateral frontal cortices and right insula. Their parents showed slightly less, but similar changes in the pattern in the amygdala connectivity. Co-occurrences of abnormal connectivity of the left amygdala with the left orbitofrontal cortex, right dorsolateral frontal cortex and right precuneus were observed in schizophrenic patients and their parents. CONCLUSIONS: Our findings suggest a potential genetic influence on structural and functional abnormalities of the amygdala in schizophrenia. Such information could help future efforts to identify the endophenotypes that characterize the complex disorder of schizophrenia.

  17. An empirical analysis of gasoline price convergence for 20 OECD countries

    Energy Technology Data Exchange (ETDEWEB)

    Bentzen, J.

    2003-07-01

    Two decades have passed now since the oil price shocks of the 1970s and since then energy prices have - apart from short periods of price instability - evolved relatively smoothly in the industrialized countries. Energy taxes in many countries differ markedly thereby causing differences in final energy prices, but as similar tax levels are becoming more common, e.g. in the European Union, convergence concerning energy prices might be expected to appear. In the present paper national gasoline price data covering the time period since the 1970s for a sample of OECD countries are used in order to test for this often addressed topic of convergence. The empirical part of the paper applies different time series based tests of convergence, where gasoline prices exhibit convergence for most OECD-Europe countries in the case where US$ is used for measurement of the energy prices indicating a convergence or tax harmonization process is taking place for these countries. (au)

  18. In silico peptide-binding predictions of passerine MHC class I reveal similarities across distantly related species, suggesting convergence on the level of protein function

    DEFF Research Database (Denmark)

    Follin, Elna; Karlsson, Maria; Lundegaard, Claus

    2013-01-01

    compared to most mammals. To elucidate the reason for this large number of genes, we compared 14 MHC class I alleles (α1–α3 domains), from great reed warbler, house sparrow and tree sparrow, via phylogenetic analysis, homology modelling and in silico peptide-binding predictions to investigate......, there were also overlapping peptide-binding specificities in the allomorphs from house sparrow and great reed warbler, although these species diverged 30 MYA. This overlap was not found in a tree based on amino acid sequences. Our interpretation is that convergent evolution on the level of the protein...... function, possibly driven by selection from shared pathogens, has resulted in allomorphs with similar peptide-binding repertoires, although trans-species evolution in combination with gene conversion cannot be ruled out....

  19. STABILITY AND SUPER CONVERGENCE ANALYSIS OF ADI-FDTD FOR THE 2D MAXWELL EQUATIONS IN A LOSSY MEDIUM

    Institute of Scientific and Technical Information of China (English)

    Gao Liping

    2012-01-01

    Several new energy identities of the two dimensional(2D) Maxwell equations in a lossy medium in the case of the perfectly electric conducting boundary conditions are proposed and proved.These identities show a new kind of energy conservation in the Maxwell system and provide a new energy method to analyze the alternating direction implicit finite difference time domain method for the 2D Maxwell equations (2D-ADI-FDTD).It is proved that 2D-ADI-FDTD is approximately energy conserved,unconditionally stable and second order convergent in the discrete L2 and H1 norms,which implies that 2D-ADI-FDTD is super convergent.By this super convergence,it is simply proved that the error of the divergence of the solution of 2D-ADI-FDTD is second order accurate.It is also proved that the difference scheme of 2D-ADI-FDTD with respect to time t is second order convergent in the discrete H1 norm.Experimental results to confirm the theoretical analysis on stability,convergence and energy conservation are presented.

  20. Convergence Analysis of the Numerical Solution for Cathode Design of Aero-engine Blades in Electrochemical Machining

    Institute of Scientific and Technical Information of China (English)

    Li Zhiyong; Niu Zongwei

    2007-01-01

    As a main difficult problem encountered in electrochemical machining (ECM), the cathode design is tackled, at present, with various numerical analysis methods such as finite difference, finite element and boundary element methods. Among them, the finite element method presents more flexibility to deal with the irregularly shaped workpieces. However, it is very difficult to ensure the convergence of finite element numerical approach. This paper proposes an accurate model and a finite element numerical approach of cathode design based on the potential distribution in inter-electrode gap. In order to ensure the convergence of finite element numerical approach and increase the accuracy in cathode design, the cathode shape should be iterated to eliminate the design errors in computational process. Several experiments are conducted to verify the macbining accuracy of the designed cathode. The experimental results have proven perfect convergence and good computing accuracy of the proposed finite element numerical approach by the high surface quality and dimensional accuracy of the machined blades.

  1. A convergence analysis of SOR iterative methods for linear systems with weak H-matrices

    Directory of Open Access Journals (Sweden)

    Zhang Cheng-yi

    2016-01-01

    Full Text Available It is well known that SOR iterative methods are convergent for linear systems, whose coefficient matrices are strictly or irreducibly diagonally dominant matrices and strong H-matrices (whose comparison matrices are nonsingular M-matrices. However, the same can not be true in case of those iterative methods for linear systems with weak H-matrices (whose comparison matrices are singular M-matrices. This paper proposes some necessary and sufficient conditions such that SOR iterative methods are convergent for linear systems with weak H-matrices. Furthermore, some numerical examples are given to demonstrate the convergence results obtained in this paper.

  2. Convergence analysis of filtered-X LMS algorithm with secondary path modeling error

    Institute of Scientific and Technical Information of China (English)

    SUN Xu; CHEN Duanshi

    2003-01-01

    A more relaxed sufficient condition for the convergence of filtered-X LMS (FXLMS)algorithm is presented. It is pointed out that if some positive real condition for secondary pathtransfer function and its estimates is satisfied within all the frequency bands, FXLMS algorithmconverges whatever the reference signal is like. But if the above positive real condition is satisfiedonly within some frequency bands, the convergence of FXLMS algorithm is dependent on thedistribution of power spectral density of the reference signal, and the convergence step size isdetermined by the distribution of some specific correlation matrix eigenvalues.Applying the conclusion above to the Delayed LMS (DLMS) algorithm, it is shown thatDLMS algorithm with some error of time delay estimation converges in certain discrete fre-quency bands, and the width of which are determined only by the "time-delay estimation errorfrequency" which is equal to one fourth of the inverse of estimated error of the time delay.

  3. Ultra-performance convergence chromatography (UPC²) method for the analysis of biogenic amines in fermented foods.

    Science.gov (United States)

    Gong, Xiao; Qi, Ningli; Wang, Xiaoxi; Lin, Lijing; Li, Jihua

    2014-11-01

    A rapid ultra-performance convergence chromatography (UPC(2)) method for the determination of eight biogenic amines (spermine, spermidine, putrescine, cadaverine, tryptamine, phenylethylamine, histamine and tyramine) in fermented foods was developed. The amines were pre-column derivatized with dansyl chloride and separated on UPC(2) system with a HSS C18 SB column (3.0 × 100 mm, 1.8 μm) using gradient elution with a binary system of CO2 and n-hexane:isopropanol:ammonium hydroxide (70:30:0.15, v/v/v), back pressure of 2,000 psi, flow rate of 2.0 ml/min and DAD detection at 254 nm. The result showed excellent linearity (r=0.9995-1.0000). Limits of detection (LOD) and limits of quantification (LOQ) were 21-67 ng/L and 72-224 ng/L, respectively. Relative standard deviations (RSD) for repeatability and reproducibility were 0.21-0.87% and 1.98-4.02%, respectively. Furthermore, this method was successfully applied to analysis of biogenic amines in Yulu and Sufu samples.

  4. An improved method for TAL effectors DNA-binding sites prediction reveals functional convergence in TAL repertoires of Xanthomonas oryzae strains.

    Directory of Open Access Journals (Sweden)

    Alvaro L Pérez-Quintero

    Full Text Available Transcription Activators-Like Effectors (TALEs belong to a family of virulence proteins from the Xanthomonas genus of bacterial plant pathogens that are translocated into the plant cell. In the nucleus, TALEs act as transcription factors inducing the expression of susceptibility genes. A code for TALE-DNA binding specificity and high-resolution three-dimensional structures of TALE-DNA complexes were recently reported. Accurate prediction of TAL Effector Binding Elements (EBEs is essential to elucidate the biological functions of the many sequenced TALEs as well as for robust design of artificial TALE DNA-binding domains in biotechnological applications. In this work a program with improved EBE prediction performances was developed using an updated specificity matrix and a position weight correction function to account for the matching pattern observed in a validation set of TALE-DNA interactions. To gain a systems perspective on the large TALE repertoires from X. oryzae strains, this program was used to predict rice gene targets for 99 sequenced family members. Integrating predictions and available expression data in a TALE-gene network revealed multiple candidate transcriptional targets for many TALEs as well as several possible instances of functional convergence among TALEs.

  5. Private selective sweeps identified from next-generation pool-sequencing reveal convergent pathways under selection in two inbred Schistosoma mansoni strains.

    Directory of Open Access Journals (Sweden)

    Julie A J Clément

    Full Text Available BACKGROUND: The trematode flatworms of the genus Schistosoma, the causative agents of schistosomiasis, are among the most prevalent parasites in humans, affecting more than 200 million people worldwide. In this study, we focused on two well-characterized strains of S. mansoni, to explore signatures of selection. Both strains are highly inbred and exhibit differences in life history traits, in particular in their compatibility with the intermediate host Biomphalaria glabrata. METHODOLOGY/PRINCIPAL FINDINGS: We performed high throughput sequencing of DNA from pools of individuals of each strain using Illumina technology and identified single nucleotide polymorphisms (SNP and copy number variations (CNV. In total, 708,898 SNPs were identified and roughly 2,000 CNVs. The SNPs revealed low nucleotide diversity (π = 2 × 10(-4 within each strain and a high differentiation level (Fst = 0.73 between them. Based on a recently developed in-silico approach, we further detected 12 and 19 private (i.e. specific non-overlapping selective sweeps among the 121 and 151 sweeps found in total for each strain. CONCLUSIONS/SIGNIFICANCE: Functional annotation of transcripts lying in the private selective sweeps revealed specific selection for functions related to parasitic interaction (e.g. cell-cell adhesion or redox reactions. Despite high differentiation between strains, we identified evolutionary convergence of genes related to proteolysis, known as a key virulence factor and a potential target of drug and vaccine development. Our data show that pool-sequencing can be used for the detection of selective sweeps in parasite populations and enables one to identify biological functions under selection.

  6. CFD analysis of flow phenomena inside thermo vapor compressor influenced by operating conditions and converging duct angles

    Energy Technology Data Exchange (ETDEWEB)

    Jeong, Hyo Min; Utomo, Tony; Ji, Myoung Kuk; Lee, Yong Hun; Lee, Gyeong Hwan; Chung, Han Shik [Gyeongsang National University, Tongyeong (Korea, Republic of)

    2009-09-15

    A thermo vapor compressor is simply a steam ejector employed in a multi effect desalination process. A greater understanding of flow phenomena inside an ejector plays an important role in its performance improvement. In this paper, CFD investigation has been carried out to study the flow structure inside a steam ejector. This research revealed the influence of operating pressures and ejector geometries on the flow structure and the performance of a steam ejector. The CFD results were verified with available experimental data. The angle of the converging duct as the geometry parameter was varied as 0 .deg., 0.5 .deg., 1 .deg., 2 .deg., 3.5 .deg. and 4.5 .deg.. The best performance was obtained by the ejector with converging duct angle of 1 .deg.

  7. Convergence Nanorobot Analysis for Radiation Therapy-Industrial Innovations in Nuclear Engineering

    Energy Technology Data Exchange (ETDEWEB)

    Woo, Taeho [Yonsei Univ., Wonju (Korea, Republic of)

    2015-10-15

    The important step of the commercialization is the make the prototype nanorobot where lots of applications could be introduced for the industry. For the much more advanced operations of the nanorobot, it is needed to imagine the strategy for the operation in the non-regular shaped organs like the lung which shows the different feature following breaths. The biological stuffs are usually in the irregular shape and could be changed by the external force or the infected viruses. The biological substance could be made by the amorphous material which is used frequently in the industry. The antibody reaction is a particular matter which could be happen in the human body. So, the adaptations of the nanorobot could be increased for the practical purposed. Fig. 7 is the newly imagined convergence nuclear technology with nanorobotics for nuclear engineering fields in which many kinds of applications are imagined. Following the new applications of the nanorobot, it is possible to challenge for the difficult matters in the conventional nuclear industry. Fig. 8 shows the historic mistakes in commercialized nuclear power plants (NPPs) considering the nuclear reactor analysis and safety system induced by the accident. Firstly, the non-matched flux shapes made by the multiplications of Bessel function and cosine function by the cylindrical core shape, which is different from the spherical or rectangular core shape, couldn't describe the exact flux shape. Secondly, the safety system installed to start in the accident is the piping-based injection equipment. However, the safety injection systems have failed in three major sever accidents as Three Mile Island (TMI), Chernobyl, and Fukushima cases due to the significant piping failures.

  8. Global Convergence Analysis of Non-Crossover Genetic Algorithm and Its Application to Optimization

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    Selection, crossover, and mutation are three main operators of the canonical genetic algorithm (CGA). This paper presents a new approach to the genetic algorithm. This new approach applies only to mutation and selection operators. The paper proves that the search process of the non-crossover genetic algorithm (NCGA) is an ergodic homogeneous Markov chain. The proof of its convergence to global optimum is presented. Some nonlinear multi-modal optimization problems are applied to test the efficacy of the NCGA. NP-hard traveling salesman problem (TSP) is cited here as the benchmark problem to test the efficiency of the algorithm. The simulation result shows that NCGA achieves much faster convergence speed than CGA in terms of CPU time. The convergence speed per epoch of NCGA is also faster than that of CGA.

  9. Spatial Growth Regressions for the convergence analysis of renewable energy consumption in Europe

    Directory of Open Access Journals (Sweden)

    Lara Fontanella

    2013-10-01

    Full Text Available In recent years there has been an increasing awareness on problems related to the economic growth and on the conditions under which some socio-economic variables measured on European countries tend to converge over time towards a common level. This paper is concerned with the use of energy from renewable sources and considers the extent to which EU countries meet the binding commitment to reach a fifth of energy consumption from renewable sources by 2020. By discussing empirical results on the economic growth pattern of 28 countries in the period 1995-2010, we make use of several spatial growth regression models. We show that the proposed models are able to capture the complexity of the phenomenon including the possibility of estimating sitespecific convergence parameters and the identification of convergence clubs.

  10. Convergence Analysis of Forgetting Gradient Algorithm by Using Martingale Hyperconvergence Theorem

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    The stochastic gradient (SG) algorithm has less of a computational burden than the least squares algorithms, but it can not track time-varying parameters and has a poor convergence rate. In order to improve the tracking properties of the SG algorithm, the forgetting gradient (FG) algorithm is presented, and its convergence is analyzed by using the martingale hyperconvergence theorem. The results show that: (1) for time-invariant deterministic systems, the parameter estimates given by the FG algorithm converge consistently to their true values; (2) for stochastic time-varying systems, the parameter tracking error is bounded, that is, the parameter tracking error is small when both the parameter change rate and the observation noise are small.

  11. Convergence Analysis of Wireless Remote Iterative Learning Control Systems with Dropout Compensation

    Directory of Open Access Journals (Sweden)

    Li-xun Huang

    2013-01-01

    Full Text Available The wireless remote iterative learning control (ILC system with random data dropouts is considered. The data dropout is viewed as a binary switching sequence which obeys the Bernoulli distribution. In order to eliminate the effect of data dropouts on the convergence property of output error, the signal at the same time with the lost one but in the last iteration is used to compensate the data dropout at the actuator. With the dropout compensation, the convergence property of output error is analyzed by studying the element values of system transition matrix. Finally, some simulation results are given to illustrate the validity of the proposed method.

  12. The convergent and discriminant validity of burnout measures in sport: a multi-trait/multi-method analysis.

    Science.gov (United States)

    Cresswell, Scott L; Eklund, Robert C

    2006-02-01

    Athlete burnout research has been hampered by the lack of an adequate measurement tool. The Athlete Burnout Questionnaire (ABQ) and the Maslach Burnout Inventory General Survey (MBI-GS) are two recently developed self-report instruments designed to assess burnout. The convergent and discriminant validity of the ABQ and MBI-GS were assessed through multi-trait/multi-method analysis with a sporting population. Overall, the ABQ and the MBI-GS displayed acceptable convergent validity with matching subscales highly correlated, and satisfactory internal discriminant validity with lower correlations between non-matching subscales. Both scales also indicated an adequate discrimination between the concepts of burnout and depression. These findings add support to previous findings in non-sporting populations that depression and burnout are separate constructs. Based on the psychometric results, construct validity analysis and practical considerations, the results support the use of the ABQ to assess athlete burnout.

  13. The Dependent Converging Instrument Approach Procedure: An Analysis of its Safety and Applicability

    Science.gov (United States)

    1992-11-01

    scenarios, this feature leads to a slightly more conservative measure of the predicited separation (i.e., smaller separation). 3.4.2 Determination of Headwind...for the DCIA procedure may facilitate the maintenance of the independence of one parallel approach, with dependent converging approaches to the other

  14. Conditional Convergence of Nonresident Tuition Rates at Public Research Universities: A Panel Data Analysis

    Science.gov (United States)

    Titus, Marvin A.; Vamosiu, Adriana; Gupta, Anubha

    2015-01-01

    The current study examines how nonresident tuition among public research universities has converged toward a national average over the 1987-2006 time period in the USA. Using dynamic fixed-effect panel modeling estimated via GMM (and instrumental variables fixed-effect model to account for endogeneity), we inquire (1) how do competitive market…

  15. CONVERGENCE RATE ANALYSIS OF MULTIPLICATIVE SCHWARZ ALGORITHM FOR ELLIPTIC VARIATIONAL INEQUALITIES

    Institute of Scientific and Technical Information of China (English)

    ZENG Jinping; ZHOU Shuzi; WANG Lieheng

    2001-01-01

    Considering multiplicative Schwarz algorithm for solving algebraic obstacleproblems, we show the geometric convergence of the algorithm by the use of discretemaximun principle. We also get a decay rate bound independent of the meshsize for theiterative error and illustrate the method by some numerical experiments.

  16. Convergence analysis of evolutionary algorithms that are based on the paradigm of information geometry.

    Science.gov (United States)

    Beyer, Hans-Georg

    2014-01-01

    The convergence behaviors of so-called natural evolution strategies (NES) and of the information-geometric optimization (IGO) approach are considered. After a review of the NES/IGO ideas, which are based on information geometry, the implications of this philosophy w.r.t. optimization dynamics are investigated considering the optimization performance on the class of positive quadratic objective functions (the ellipsoid model). Exact differential equations describing the approach to the optimizer are derived and solved. It is rigorously shown that the original NES philosophy optimizing the expected value of the objective functions leads to very slow (i.e., sublinear) convergence toward the optimizer. This is the real reason why state of the art implementations of IGO algorithms optimize the expected value of transformed objective functions, for example, by utility functions based on ranking. It is shown that these utility functions are localized fitness functions that change during the IGO flow. The governing differential equations describing this flow are derived. In the case of convergence, the solutions to these equations exhibit an exponentially fast approach to the optimizer (i.e., linear convergence order). Furthermore, it is proven that the IGO philosophy leads to an adaptation of the covariance matrix that equals in the asymptotic limit-up to a scalar factor-the inverse of the Hessian of the objective function considered.

  17. Convergence, Admissibility, and Fit of Alternative Confirmatory Factor Analysis Models for MTMM Data

    Science.gov (United States)

    Lance, Charles E.; Fan, Yi

    2016-01-01

    We compared six different analytic models for multitrait-multimethod (MTMM) data in terms of convergence, admissibility, and model fit to 258 samples of previously reported data. Two well-known models, the correlated trait-correlated method (CTCM) and the correlated trait-correlated uniqueness (CTCU) models, were fit for reference purposes in…

  18. A SPECTRAL METHOD FOR PANTOGRAPH-TYPE DELAY DIFFERENTIAL EQUATIONS AND ITS CONVERGENCE ANALYSIS

    Institute of Scientific and Technical Information of China (English)

    Ishtiaq Ali; Hermann Brunner; Tao Tang

    2009-01-01

    We propose a novel numerical approach for delay differential equations with vanishing proportional delays based on spectral methods. A Legendre-collocation method is employed to obtain highly accurate numerical approximations to the exact solution. It is proved theoretically and demonstrated numerically that the proposed method converges exponentially provided that the data in the given pantograph delay differential equation are smooth.

  19. A new convergence analysis and perturbation resilience of some accelerated proximal forward-backward algorithms with errors

    CERN Document Server

    Reem, Daniel

    2015-01-01

    Many problems in science and engineering involve, as part of their solution process, the consideration of a separable function which is the sum of two convex functions, one of them possibly non-smooth. Recently a few works have discussed inexact versions of several accelerated proximal methods aiming at solving this minimization problem. This paper shows that inexact versions of a method of Beck and Teboulle (FISTA) preserve, in a Hilbert space setting, the same (non-asymptotic) rate of convergence under some assumptions on the decay rate of the error terms. The notion of inexactness discussed here seems to be rather simple, but, interestingly, when comparing to related works, similar decay rates of the errors terms yield similar convergence rates. The derivation sheds some light on the somewhat mysterious origin of some parameters which appear in various accelerated methods. A consequence of the analysis is that the accelerated method is perturbation resilient, making it suitable, in principle, for the super...

  20. SOARCA Peach Bottom Atomic Power Station Long-Term Station Blackout Uncertainty Analysis: Convergence of the Uncertainty Results

    Energy Technology Data Exchange (ETDEWEB)

    Bixler, Nathan E.; Osborn, Douglas.; Sallaberry, Cedric Jean-Marie; Eckert-Gallup, Aubrey Celia; Mattie, Patrick D.; Ghosh, S. Tina

    2014-02-01

    This paper describes the convergence of MELCOR Accident Consequence Code System, Version 2 (MACCS2) probabilistic results of offsite consequences for the uncertainty analysis of the State-of-the-Art Reactor Consequence Analyses (SOARCA) unmitigated long-term station blackout scenario at the Peach Bottom Atomic Power Station. The consequence metrics evaluated are individual latent-cancer fatality (LCF) risk and individual early fatality risk. Consequence results are presented as conditional risk (i.e., assuming the accident occurs, risk per event) to individuals of the public as a result of the accident. In order to verify convergence for this uncertainty analysis, as recommended by the Nuclear Regulatory Commission’s Advisory Committee on Reactor Safeguards, a ‘high’ source term from the original population of Monte Carlo runs has been selected to be used for: (1) a study of the distribution of consequence results stemming solely from epistemic uncertainty in the MACCS2 parameters (i.e., separating the effect from the source term uncertainty), and (2) a comparison between Simple Random Sampling (SRS) and Latin Hypercube Sampling (LHS) in order to validate the original results obtained with LHS. Three replicates (each using a different random seed) of size 1,000 each using LHS and another set of three replicates of size 1,000 using SRS are analyzed. The results show that the LCF risk results are well converged with either LHS or SRS sampling. The early fatality risk results are less well converged at radial distances beyond 2 miles, and this is expected due to the sparse data (predominance of “zero” results).

  1. Implementation of Modified Conjugate Gradient Algorithm and Analysis of Convergence in Electromagnetic Tomography Lab System

    Directory of Open Access Journals (Sweden)

    Li Liu

    2013-06-01

    Full Text Available Electromagnetic tomography technology is a new process tomography technology. The aim of this study is to develop a new image reconstruction algorithm suitable to electromagnetic tomography and verify its convergence. The advantages and development of electromagnetic tomography technology and image reconstruction algorithms are introduced briefly. Based on conjugate gradient algorithm, modified conjugate gradient algorithm for Electromagnetic Tomography (EMT is proposed. Convergence of the modified conjugate gradient algorithm is analyzed. In the light of the lab electromagnetic tomography system, modified conjugate gradient algorithm for reconstructing images is verified. By evaluation of image error and the relevance, regularization algorithm, Landweber algorithm, conjugate gradient algorithm and modified conjugate gradient algorithm are compared. It can draw the conclusion that for different flow patterns, modified conjugate gradient algorithm is superior to other algorithms in the 8 coils electromagnetic tomography lab system.

  2. A Local and Global Search Combined Particle Swarm Optimization Algorithm and Its Convergence Analysis

    Directory of Open Access Journals (Sweden)

    Weitian Lin

    2014-01-01

    Full Text Available Particle swarm optimization algorithm (PSOA is an advantage optimization tool. However, it has a tendency to get stuck in a near optimal solution especially for middle and large size problems and it is difficult to improve solution accuracy by fine-tuning parameters. According to the insufficiency, this paper researches the local and global search combine particle swarm algorithm (LGSCPSOA, and its convergence and obtains its convergence qualification. At the same time, it is tested with a set of 8 benchmark continuous functions and compared their optimization results with original particle swarm algorithm (OPSOA. Experimental results indicate that the LGSCPSOA improves the search performance especially on the middle and large size benchmark functions significantly.

  3. Analysis and Convergence of the MAC Scheme. 1. The Linear Problem

    Science.gov (United States)

    1991-03-01

    the velocity and pressure respectively. 17 Key Words iSuggested by Authorfsi) 18 Distribution Statement staggered meshes, covolume methods, CFD 34...approximations do in fact converge to the exact solutions of the flow equations. Estimates using mesh dependent norms analogous to the standard H 1 and...L’ norms are given for the velocity and pressure respectively. 1This research was supported by the National Aeronautics and Space Administration under

  4. The analysis of convergence process of voivodships' efficiency in Poland using the DEA metod

    OpenAIRE

    Wozniak, Dariusz; Czarnecki, Piotr; Szarota, Robert

    2011-01-01

    Since 1999 - when the new administrative division in Poland was introduced - it has been possible to measure and compare standard of living between Polish and other European regions (NUTS II). In 2004 Poland has joined the European Union. Since that year voivodships have become main beneficiaries of the EU funds. The essential part of that aid is related to the EU cohesion policy (convergence objective). According to the fifth cohesion report "cohesion policy has made a significant contributi...

  5. The Convergence in Spatial Tasks

    OpenAIRE

    Vladimir P. Kulagin; Victor Y. Tsvetkov; Maiya V. Maksimova

    2013-01-01

    The article reveals the problem of convergence of direct and inverse problems in Earth Sciences, describes the features and application of these problems, discloses analytical features of direct and inverse problems. The convergence criteria and conditions for convergence were presented. This work is supported by the Grant of the Government of the Russian Federation for support of scientific research, implemented under the supervision of leading scientists in Russian institutions of higher ed...

  6. Computing the Stackelberg/Nash equilibria using the extraproximal method: Convergence analysis and implementation details for Markov chains games

    Directory of Open Access Journals (Sweden)

    Trejo Kristal K.

    2015-06-01

    Full Text Available In this paper we present the extraproximal method for computing the Stackelberg/Nash equilibria in a class of ergodic controlled finite Markov chains games. We exemplify the original game formulation in terms of coupled nonlinear programming problems implementing the Lagrange principle. In addition, Tikhonov’s regularization method is employed to ensure the convergence of the cost-functions to a Stackelberg/Nash equilibrium point. Then, we transform the problem into a system of equations in the proximal format. We present a two-step iterated procedure for solving the extraproximal method: (a the first step (the extra-proximal step consists of a “prediction” which calculates the preliminary position approximation to the equilibrium point, and (b the second step is designed to find a “basic adjustment” of the previous prediction. The procedure is called the “extraproximal method” because of the use of an extrapolation. Each equation in this system is an optimization problem for which the necessary and efficient condition for a minimum is solved using a quadratic programming method. This solution approach provides a drastically quicker rate of convergence to the equilibrium point. We present the analysis of the convergence as well the rate of convergence of the method, which is one of the main results of this paper. Additionally, the extraproximal method is developed in terms of Markov chains for Stackelberg games. Our goal is to analyze completely a three-player Stackelberg game consisting of a leader and two followers. We provide all the details needed to implement the extraproximal method in an efficient and numerically stable way. For instance, a numerical technique is presented for computing the first step parameter (λ of the extraproximal method. The usefulness of the approach is successfully demonstrated by a numerical example related to a pricing oligopoly model for airlines companies.

  7. Bandwidth Reduction and Convergence Analysis of Extremum Seeking Control with Feedback Encoding

    Directory of Open Access Journals (Sweden)

    Vikum Sri Wijesinghe

    2016-11-01

    Full Text Available Frequently a physical plant of a control system has an optimum operating point such as the spark (or injection time of an internal combustion engine that results in maximum torque. Extremum Seeking Control (ESC is a method of adaptive control capable of locating and maintaining a plant at such an optimum operating point in real time. It is capable of doing so with minimal a priori knowledge of the plant and can also track slowly varying changes. Input perturbed ESC schemes that use periodic dither signals have the disadvantage of requiring a high bandwidth for sampling and correlating the plant output with the dither signal. If the feedback path were to be implemented over a packet switched communication network, the high bandwidth requirement could result in increased congestion and consequently packet delays and dropouts. As a solution encoding using sporadic (aperiodic sampling techniques can be used in the feedback path of the ESC scheme to reduce the required bandwidth. However, in order to ensure convergence of the ESC scheme with encoding, the effect of the signal reconstruction error due to encoding on the critical correlation stage has to be investigated. The contribution of this paper is an investigation of the convergence requirements and bandwidth performance of two encoding schemes; Memory Based Event Triggering (MBET and Event Triggered Adaptive Differential Modulation (ETADM. The results show that MBET can fail for objective functions with plateaus. ETADM fails when the number of ETADM steps used for reconstructing the plant output per perturbation cycle are too low to allow correlation. In terms of bandwidth reduction MBET performs better than ETADM (97% and 70% respectively. However, the use of MBET results in a longer convergence time.

  8. Convergence analysis on Browder-Tikhonov regularization for second-order evolution hemivariational inequality

    Institute of Scientific and Technical Information of China (English)

    Yibin XIAO; Guoji TANG; Xianjun LONG; Nanjing HUANG

    2015-01-01

    This paper studies the Browder-Tikhonov regularization of a second-order evolution hemivariational inequality (SOEHVI) with non-coercive operators. With dual-ity mapping, the regularized formulations and a derived first-order evolution hemivaria-tional inequality (FOEHVI) for the problem considered are presented. By applying the Browder-Tikhonov regularization method to the derived FOEHVI, a sequence of regular-ized solutions to the regularized SOEHVI is constructed, and the strong convergence of the whole sequence of regularized solutions to a solution to the problem is proved.

  9. Convergence Analysis of Iterative Sequences for a Pair of Mappings in Banach Spaces

    Institute of Scientific and Technical Information of China (English)

    Liu Chuan ZENG; N. C. WONG; J. C. YAO

    2008-01-01

    Let C be a nonempty closed convex subset of a real Banach space E Let S :C→C be a quasi-nonexpansive mapping,let T :C→C be an asymptotically demicontractive and uniformly Lipschitzian mapping,and let F := {x∈C :Sx =x and Tx x }≠φ.Let {xn } n≥0 be the sequence generated from an arbitrary x∈C by xn+1 =(1 -cn) Sxn +cnTnxn,n ≥0 . We prove the necessary and su .cient conditions for the strong convergence of the iterative sequence {xn }to an element of F These extend and improve the recent results of Moore and Nnoli.

  10. CONVERGENCE ANALYSIS FOR SYSTEM OF EQUILIBRIUM PROBLEMS AND LEFT BREGMAN STRONGLY RELATIVELY NONEXPANSIVE MAPPING

    Institute of Scientific and Technical Information of China (English)

    Yekini SHEHU

    2014-01-01

    In this paper, we prove strong convergence theorems for approximation of a fixed point of a left Bregman strongly relatively nonexpansive mapping which is also a solution to a finite system of equilibrium problems in the framework of reflexive real Banach spaces. We also discuss the approximation of a common fixed point of a family of left Bregman strongly nonexpansive mappings which is also solution to a finite system of equilibrium problems in reflexive real Banach spaces. Our results complement many known recent results in the literature.

  11. Geomorphological analysis of the drainage system on the active convergent system in Azerbaijan, NW Iran

    Science.gov (United States)

    Kaveh Firouz, Amaneh; Burg, Jean-Pierre; Giachetta, Emanuele

    2016-04-01

    Rivers are important landforms to reconstruct recent tectonic history because they are sensitive to surface movements, especially uplift and tilting. The most important drainage basins of NW Iran are, from north to south, the Arax River, the Urmia Lake and the Ghezel Ozan River catchment. The morphology of the two adjacent catchments draining into the Caspian Sea, the Arax and Ghezel Ozan were studied to better understand the active tectonics and the effect of fault activity on morphology and erosion rate of NW Iran. We performed a quantitative analysis of channel steepness and concavity, from slope-area plots calculated from digital elevation model. This information has been combined with GPS velocity vectors and seismicity. Both catchments developed under uniform climate conditions. Results show that the two rivers are in morphological disequilibrium; they exhibit profiles with prominent convexities and knickpoints. The Arax River shows higher channel steepness and concavity index in downstream part of the profile. Distribution of knickpoints show scattered elevation between 700m and 3000m. GPS rates display shortening 10 ± 2 mma-1 and 14 ± 2 mma-1 in upstream and downstream, respectively. The river profiles of Ghezel Ozan River and its tributaries reveal more disequilibrium downstream where channel steepness and concavity index are higher than upstream. Most knickpoints occur between 1000m and 2000m. The amount of shortening by GPS measurement changes from upstream 13 ± 2 mma-1to downstream 14 ± 2 mma-1. Recorded earthquakes, such as Rudbar earthquake (Mw=7.3, 1990), are more frequent downstream. The Urmia Lake is surrounded by many small and large catchments. Only major catchments were considered for the analysis. One of the most active faults, the north Tabriz fault, corresponds to a major knickpoints on the Talkhe rud River. Concordance between river profile analysis, GPS and seismotectonic records suggests that the characteristics of the river profiles

  12. AN ANALYSIS OF THE FISCAL CONVERGENCE CRITERIA IN THE EUROPEAN UNION IN TERMS OF THE SUSTAINABILITY

    Directory of Open Access Journals (Sweden)

    Iulia Andreea Bucur

    2014-01-01

    Full Text Available Although the convergence criteria in the Maastricht Treaty led to the creation of the EMU and the euro area has resisted more than some of its critics believed, in the context of major macroeconomic imbalances, the issue of nominal convergence has been the subject of numerous research. This paper aims to analyze the capacity of fiscal criteria to reflect the ability of EU Member States to achieve economic development for the integration in the EMU and to comply strict fiscal policy that governing its operation. In the context of certain technical deficiencies of fiscal criteria, we analyzed the developments of budget deficit and gross public debt in the EU during 2000 and 2012. The results show that until 2007 the EU economies were able, overall, to meet the budget deficit criteria, but due to the financial crisis and the prolonged slowdown in economic growth, the fiscal balance had an unfavorable evolution since 2008, while the evolution of the gross public debt has worsened increasingly. Due to this pressure situation on the sustainability of public finances, examining the adoption, application and enforcement of the fiscal policy rules expressed by the evolution of the Fiscal Rule Index for the period 2000-2011, the importance of rules in the fiscal management of the Union and especially of the euro area appears increasingly obvious.

  13. [Comparative analysis of spatial EEG organization on models of non-verbal divergent and convergent thinking].

    Science.gov (United States)

    Sviderskaia, N E; Antonov, A G; Butneva, L S

    2007-01-01

    Features of neurophysiological organization of two main thinking types playing different roles in creative processes, i.e., divergent and convergent were studied with participation of 30 right-handed male subjects at the age from 30 to 50 years. Two tests were presented: (1) creation of many visual images on the basis of two simple geometrical figures (the model of divergent thinking) and (2) classification of a figure element with one of the offered standard samples (convergent thinking). The number of created images or correctly classified elements for five minutes served a criterion of performance productivity. It was found that performance of the divergent test with high productivity (as compared to low productivity) was characterized by a greater increase in non-linear interactions between the cortical potentials, especially in the axis right frontal--left occipital areas. At the same time, under conditions of high productivity, the number of active narrow-frequency spectral-coherent EEG bands increased. The data confirm the notion of neurophysiological organization of creative processes, according to which creative processes require the intensification of retrieval operations (both conscious and unconscious), based on extensive interhemispheric interaction and involvement of a system of EEG coherent structures oscillating with different frequencies.

  14. Analysis of different feature selection criteria based on a covariance convergence perspective for a SLAM algorithm.

    Science.gov (United States)

    Auat Cheein, Fernando A; Carelli, Ricardo

    2011-01-01

    This paper introduces several non-arbitrary feature selection techniques for a Simultaneous Localization and Mapping (SLAM) algorithm. The feature selection criteria are based on the determination of the most significant features from a SLAM convergence perspective. The SLAM algorithm implemented in this work is a sequential EKF (Extended Kalman filter) SLAM. The feature selection criteria are applied on the correction stage of the SLAM algorithm, restricting it to correct the SLAM algorithm with the most significant features. This restriction also causes a decrement in the processing time of the SLAM. Several experiments with a mobile robot are shown in this work. The experiments concern the map reconstruction and a comparison between the different proposed techniques performance. The experiments were carried out at an outdoor environment composed by trees, although the results shown herein are not restricted to a special type of features.

  15. Convergence analysis of a balalncing domain decomposition method for solving interior Helmholtz equations

    Energy Technology Data Exchange (ETDEWEB)

    Li,Jing; Tu, Xuemin

    2008-12-10

    A variant of balancing domain decomposition method by constraints (BDDC) is proposed for solving a class of indefinite system of linear equations, which arises from the finite element discretization of the Helmholtz equation of time-harmonic wave propagation in a bounded interior domain. The proposed BDDC algorithm is closely related to the dual-primal finite element tearing and interconnecting algorithm for solving Helmholtz equations (FETI-DPH). Under the condition that the diameters of the subdomains are small enough, the rate of convergence is established which depends polylogarithmically on the dimension of the individual subdomain problems and which improves with the decrease of the subdomain diameters. These results are supported by numerical experiments of solving a Helmholtz equation on a two-dimensional square domain.

  16. Analysis of Different Feature Selection Criteria Based on a Covariance Convergence Perspective for a SLAM Algorithm

    Directory of Open Access Journals (Sweden)

    Fernando A. Auat Cheein

    2010-12-01

    Full Text Available This paper introduces several non-arbitrary feature selection techniques for a Simultaneous Localization and Mapping (SLAM algorithm. The feature selection criteria are based on the determination of the most significant features from a SLAM convergence perspective. The SLAM algorithm implemented in this work is a sequential EKF (Extended Kalman filter SLAM. The feature selection criteria are applied on the correction stage of the SLAM algorithm, restricting it to correct the SLAM algorithm with the most significant features. This restriction also causes a decrement in the processing time of the SLAM. Several experiments with a mobile robot are shown in this work. The experiments concern the map reconstruction and a comparison between the different proposed techniques performance. The experiments were carried out at an outdoor environment  composed by trees, although the results shown herein are not restricted to a special type of features.

  17. The Analysis of Regional Innovation Convergence of China from the Perspective of Spatial Effect%基于空间效应视角的中国区域创新收敛性分析

    Institute of Scientific and Technical Information of China (English)

    潘雄锋; 张维维

    2013-01-01

    Since Ramsey firstly studied the regional convergence based on the neo-classical economic framework in the early 1900s, the topic had become an important topic for regional evaluative economists. For instance, the convergence of technological innovation activities largely determines the degree of regional economic convergence and their influence has attracted attention from many scholars. However, existing research on the regional innovation convergence has ignored spatial interactions among regions which can not reveal spatial characteristics of regional innovation convergence. The paper studies regional innovation convergence of China by using the spatial econometrics analysis method from the perspective of spatial effect. The Moran's I spatial autocorrelation test shows that there is significant spatial autocorrelation on the regional innovation in China. This finding shows that there are significant spillover and diffusion effects on the regional innovation of China. However, the OLS analysis method previously used to estimate regional innovation convergence of China may have biased the results. Therefore, we shouldn't overlooked the potential spatial effect while testing regional innovation convergence of China. The diagnostic test results of spatial econometric model show that the spatial error model (SEM) is more suitable to estimating the regional innovation convergence of China than the spatial lag model ( SAR). SEM test shows that regional innovation of China does not show an absolute p-convergence between 1990 and 2008, but it shows conditional p-convergence. Moreover, economic development level, human capital, R&D investment, and openness are important factors to promote regional innovation convergence in China. The test results of regional innovation convergence in China reflect the implementation of non-balanced development strategy, the economic development strategy of western regions, and revitalizing old industrial bases in the northeastern region

  18. Convergence and divergence in the evolution of the APOBEC3G-Vif interaction reveal ancient origins of simian immunodeficiency viruses.

    Science.gov (United States)

    Compton, Alex A; Emerman, Michael

    2013-01-01

    Naturally circulating lentiviruses are abundant in African primate species today, yet their origins and history of transmitting between hosts remain obscure. As a means to better understand the age of primate lentiviruses, we analyzed primate genomes for signatures of lentivirus-driven evolution. Specifically, we studied the adaptive evolution of host restriction factor APOBEC3G (A3G) in Old World Monkey (OWM) species. We find recurrent mutation of A3G in multiple primate lineages at sites that determine susceptibility to antagonism by the lentiviral accessory protein Vif. Using a broad panel of SIV Vif isolates, we demonstrate that natural variation in OWM A3G confers resistance to Vif-mediated degradation, suggesting that adaptive variants of the host factor were selected upon exposure to pathogenic lentiviruses at least 5-6 million years ago (MYA). Furthermore, in members of the divergent Colobinae subfamily of OWM, a multi-residue insertion event in A3G that arose at least 12 MYA blocks the activity of Vif, suggesting an even more ancient origin of SIV. Moreover, analysis of the lentiviruses associated with Colobinae monkeys reveal that the interface of the A3G-Vif interaction has shifted and given rise to a second genetic conflict. Our analysis of virus-driven evolution describes an ancient yet ongoing genetic conflict between simian primates and lentiviruses on a million-year time scale.

  19. Multiplatform analysis of 12 cancer types reveals molecular classification within and across tissues of origin

    DEFF Research Database (Denmark)

    Hoadley, Katherine A; Yau, Christina; Wolf, Denise M

    2014-01-01

    Recent genomic analyses of pathologically defined tumor types identify "within-a-tissue" disease subtypes. However, the extent to which genomic signatures are shared across tissues is still unclear. We performed an integrative analysis using five genome-wide platforms and one proteomic platform...... on 3,527 specimens from 12 cancer types, revealing a unified classification into 11 major subtypes. Five subtypes were nearly identical to their tissue-of-origin counterparts, but several distinct cancer types were found to converge into common subtypes. Lung squamous, head and neck, and a subset...... of bladder cancers coalesced into one subtype typified by TP53 alterations, TP63 amplifications, and high expression of immune and proliferation pathway genes. Of note, bladder cancers split into three pan-cancer subtypes. The multiplatform classification, while correlated with tissue-of-origin, provides...

  20. Performance Convergence Analysis of Stock Exchanges: the Situation of the Ibovespa in the World Scenario

    Directory of Open Access Journals (Sweden)

    Paulo Rogério Faustino Matos

    2011-09-01

    Full Text Available This paper studies the behavior of the most relevant worldwide stock exchanges indices. The semiparametric time series technique proposed by Phillips and Sul (2007 is used to a panel containing 36 stock exchanges allocated in economies with different development levels situated on all continents, during the period from January 1998 to December 2007. According to the results, there is no common trend, corroborating Antzolautos et al. (2009. The traditional São Paulo Stock Exchange Index (Ibovespa is the oldest one of the group with the highest level of the trend for the dynamic transition, which is comprised by volatile indices of stock exchanges with a reasonable level of maturity, located in developing economies with high rates of inflation in Central and Latin America. The second club comprises most of the indices, characterized by a higher level of maturity and tradition of financial markets and development, located mainly in Europe, North America and Asia. The third club with only four indices, has no clear patterns. The evidence that the convergence clubs composition has macroeconomic, geographical and financial patterns can be a useful to infer about the post world financial crisis behavior of stock exchanges.

  1. New Parallaxes and a Convergence Analysis for the TW Hya Association

    CERN Document Server

    Donaldson, Jessica K; Gagné, Jonathan; Faherty, Jacqueline K; Boss, Alan P; Keiser, Sandra A

    2016-01-01

    The TW Hya Association (TWA) is a nearby stellar association with an age of $\\sim$ 5-10 Myr. This is an important age for studying the late stages of star and planet formation. We measure the parallaxes of 14 candidate members of TWA. That brings to 38 the total number of individual stars with fully measured kinematics, i.e. proper motion, radial velocity, and parallax, to describe their motions through the Galaxy. We analyze these kinematics to search for convergence to a smaller volume in the past, but we find the association is never much more compact than it is at present. We show that it is difficult to measure traceback ages for associations such as TWA that have expected velocity dispersions of 1-2 km s$^{-1}$ with typical measurement uncertainties. We also use our stellar distances and pre-main-sequence evolutionary tracks to find the average age of the association of 7.9 $\\pm$ 1.0 Myr. Additionally, our parallax measurement of TWA 32 indicates it should be considered a bona fide member of TWA. Two ne...

  2. Stability and convergence analysis of a variable order replicator-mutator process in a moving medium.

    Science.gov (United States)

    Doungmo Goufo, Emile Franc

    2016-08-21

    A more generalized approach, the concept of variable order derivative, is used to study the well-known replicator-mutator dynamics taking place in a moving medium. The biological relevance of the variable order context is explored via the language learning in social groups and stability of fixed points for the generalized model is recalled and discussed. Related graphs are plotted for different values of the derivative order γ. It happens that the threshold condition for learning accuracy symbolized by a function of payoff is a monotonically increasing function irrespective of the value of the time derivative order. Also, the limit cycles and their amplitudes are shown to vary with the value of the derivative order γ. These amplitudes become bigger as γ grows but the stability of the system is not affected. The generalized model, namely the variable order replicator-mutator dynamics in a moving medium is numerically solved via Crank-Nicholson scheme whose stability and convergence results are provided in details. An application to a variable order replicator-mutator dynamics of a population with three strategies is presented and numerical simulations are performed for some fixed values of the position variable r and the grid points. They display limit cycles appearing and disappearing in function of the values of the position r. The amplitudes of limit cycles are also proved to proportionally depend on r and the stability of the system remains unaffected. This shows the impressive effect of the transport process on the bifurcation dynamics of the model.

  3. Analysis of the turbulent flow field in a spherically convergent implosion problem

    Science.gov (United States)

    Boureima, Ismael; Ramaprabhu, Praveen; Attal, Nitesh

    2016-11-01

    We describe results from 3D, numerical simulations of a spherically convergent, implosion problem. The problem definition follows, and involves a time-dependent pressure drive that sustains the implosion of an interface in a slow-fast configuration. The simulations are performed within a spherical wedge, where the interface is initialized with multimode perturbations leading to turbulent flow. The initial stages of the implosion are dominated by the Richtymer-Meshkov (RM) instability, while the late stages involve a stagnation phase interspersed with reshocks during which both RM and Rayleigh-Taylor (RT) instabilities are observed. The simulations were performed with the FLASH code, with a mesh resolution corresponding to 512x512 zones in the (θ, ϕ) directions, and proportional gridding in the r-direction. We report on several quantities that could provide insights in to the evaluation of turbulence models including the turbulent kinetic energy, anisotropy tensor, density self-correlation, and atomic mixing among others. This work was supported in part by the (U.S.) Department of Energy (DOE) under Contract No. DE-AC52-06NA2-5396.

  4. A new convergence analysis and perturbation resilience of some accelerated proximal forward–backward algorithms with errors

    Science.gov (United States)

    Reem, Daniel; De Pierro, Alvaro

    2017-04-01

    Many problems in science and engineering involve, as part of their solution process, the consideration of a separable function which is the sum of two convex functions, one of them possibly non-smooth. Recently a few works have discussed inexact versions of several accelerated proximal methods aiming at solving this minimization problem. This paper shows that inexact versions of a method of Beck and Teboulle (fast iterative shrinkable tresholding algorithm) preserve, in a Hilbert space setting, the same (non-asymptotic) rate of convergence under some assumptions on the decay rate of the error terms The notion of inexactness discussed here seems to be rather simple, but, interestingly, when comparing to related works, closely related decay rates of the errors terms yield closely related convergence rates. The derivation sheds some light on the somewhat mysterious origin of some parameters which appear in various accelerated methods. A consequence of the analysis is that the accelerated method is perturbation resilient, making it suitable, in principle, for the superiorization methodology. By taking this into account, we re-examine the superiorization methodology and significantly extend its scope. This work was supported by FAPESP 2013/19504-9. The second author was supported also by CNPq grant 306030/2014-4.

  5. A Comparative Analysis of E-Learning Policy Formulation in the European Union and the United States: Discursive Convergence and Divergence

    Science.gov (United States)

    Erichsen, Elizabeth Roumell; Salajan, Florin D.

    2014-01-01

    This study developed a framework that compares the content and purposes of "federal" level European Union (EU) and United States (US) e-learning policy to ascertain trends, patterns, and points of convergence and divergence across the years 1994-2010. It reveals that the EU and US are applying similar rhetoric for policy framing,…

  6. High-throughput sequence analysis reveals structural diversity and improved potency among RNA inhibitors of HIV reverse transcriptase.

    Science.gov (United States)

    Ditzler, Mark A; Lange, Margaret J; Bose, Debojit; Bottoms, Christopher A; Virkler, Katherine F; Sawyer, Andrew W; Whatley, Angela S; Spollen, William; Givan, Scott A; Burke, Donald H

    2013-02-01

    Systematic evolution of ligands through exponential enrichment (SELEX) is a well-established method for generating nucleic acid populations that are enriched for specified functions. High-throughput sequencing (HTS) enhances the power of comparative sequence analysis to reveal details of how RNAs within these populations recognize their targets. We used HTS analysis to evaluate RNA populations selected to bind type I human immunodeficiency virus reverse transcriptase (RT). The populations are enriched in RNAs of independent lineages that converge on shared motifs and in clusters of RNAs with nearly identical sequences that share common ancestry. Both of these features informed inferences of the secondary structures of enriched RNAs, their minimal structural requirements and their stabilities in RT-aptamer complexes. Monitoring population dynamics in response to increasing selection pressure revealed RNA inhibitors of RT that are more potent than the previously identified pseudoknots. Improved potency was observed for inhibition of both purified RT in enzymatic assays and viral replication in cell-based assays. Structural and functional details of converged motifs that are obscured by simple consensus descriptions are also revealed by the HTS analysis. The approach presented here can readily be generalized for the efficient and systematic post-SELEX development of aptamers for down-stream applications.

  7. The Convergence Analysis on the Economic Growth and Energy Intensity Gap between Regional Sectors%The Convergence Analysis on the Economic Growth and Energy Intensity Gap between Regional Sectors

    Institute of Scientific and Technical Information of China (English)

    Qi Shaozhou; Li Kai

    2011-01-01

    In this paper, the authors have analyzed the relationship between energy intensity gap and GDP per worker gap of China's western and eastern provinces over the period 1997 2006. Using panel data model with lag adjustment, taking the above provinces and six industrial sectors (agriculture, forestry, animal husbandry, and fisheries, industry, construction industry, transport, storage and post & telecommunications, wholesale and retail trades & catering industry, and other sectors of tertiary industry.) as the investigated subjects, the authors have conducted empirical study on the convergence of GDP per worker gap and the convergence of energy intensity gap with respect to the variation of GDP per worker gap, and have concluded that: First, the GDP per worker gap of the six industrial sectors and provinces are convergent, and of this, the convergence rate of GDP per worker gap of Construction Industry is the fastest, while that of Industry is the slowest. Second, the overall energy intensity gap between eastern and western provinces is convergent, that is, with the narrowing of GDP per worker gap between eastern and western provinces, the energy intensity gap converges, but its convergence rate is slower than that of GDP per worker gap. Third, energy intensity gap between various industrial sectors of the east and the west is either convergent or divergent, and there are differences. The energy intensity gap of agriculture, forestry, animal husbandry, and fisheries, industry, and construction industry is convergent, while that of the other three industrial sectors is divergent. Fourth, the convergence of the overall energy intensity of the western provinces is not in conformity with the convergence of the various industrial sectors, and there are significant differences, indicating that the western provinces and autonomous regions should take measures to more effectively improve their overall energy utilization efficiency at the industrial sector level.

  8. The Convergence in Spatial Tasks

    Directory of Open Access Journals (Sweden)

    Vladimir P. Kulagin

    2013-01-01

    Full Text Available The article reveals the problem of convergence of direct and inverse problems in Earth Sciences, describes the features and application of these problems, discloses analytical features of direct and inverse problems. The convergence criteria and conditions for convergence were presented. This work is supported by the Grant of the Government of the Russian Federation for support of scientific research, implemented under the supervision of leading scientists in Russian institutions of higher education in the field "Space Research and Technologies" in 2011–2013.

  9. Meta-Analysis inside and outside Particle Physics: Convergence Using the Path of Least Resistance?

    Science.gov (United States)

    Jackson, Dan; Baker, Rose

    2013-01-01

    In this note, we explain how the method proposed by Hartung and Knapp provides a compromise between conventional meta-analysis methodology and "unconstrained averaging", as used by the Particle Data Group.

  10. Fiscal Convergence in an Enlarged European Union

    Directory of Open Access Journals (Sweden)

    Georgeta VINTILĂ

    2014-02-01

    Full Text Available Taxation convergence in the European Union has always been a heatedly debated issue, and it has constantly resurfaced to the limelight as more and more fiscal directive proposals are being discussed. The current study is an analysis of the evolution of taxation convergence tendencies within the European Union in recent years, using sigma-convergence and cluster analysis. Our goal is to analyze the fiscal convergence trends in the context of European Union enlargement over the last two decades.

  11. Meta-Analysis inside and outside Particle Physics: Two Traditions That Should Converge?

    Science.gov (United States)

    Baker, Rose D.; Jackson, Dan

    2013-01-01

    The use of meta-analysis in medicine and epidemiology really took off in the 1970s. However, in high-energy physics, the Particle Data Group has been carrying out meta-analyses of measurements of particle masses and other properties since 1957. Curiously, there has been virtually no interaction between those working inside and outside particle…

  12. Points of convergence between functional and formal approaches to syntactic analysis

    DEFF Research Database (Denmark)

    Bjerre, Tavs; Engels, Eva; Jørgensen, Henrik;

    2008-01-01

    respectively: The functional approach is represented by Paul Diderichsen's (1936, 1941, 1946, 1964) sætningsskema, ‘sentence model', and the formal approach is represented by analysis whose main features are common to the principles and parameters framework (Chomsky 1986) and the minimalist programme (Chomsky...

  13. Convergence among Data Sources, Response Bias, and Reliability and Validity of a Structured Job Analysis Questionnaire.

    Science.gov (United States)

    Smith, Jack E.; Hakel, Milton D.

    1979-01-01

    Examined are questions pertinent to the use of the Position Analysis Questionnaire: Who can use the PAQ reliably and validly? Must one rely on trained job analysts? Can people having no direct contact with the job use the PAQ reliably and validly? Do response biases influence PAQ responses? (Author/KC)

  14. Linear stability analysis reveals exclusion zone for sliding bed transport

    Directory of Open Access Journals (Sweden)

    Talmon Arnold M.

    2015-06-01

    Full Text Available A bend or any another pipe component disturbs solids transport in pipes. Longitudinal pressure profiles downstream of such a component may show a stationary transient harmonic wave, as revealed by a recent settling slurry laboratory experiment. Therefore the fundamental transient response of the two-layer model for fully stratified flow is investigated as a first approach. A linear stability analysis of the sliding bed configuration is conducted. No stationary transient harmonic waves are found in this analysis, but adaptation lengths for exponential recovery are quantified. An example calculation is given for a 0.1 m diameter pipeline.

  15. Invoking Thomas Kuhn: What Citation Analysis Reveals about Science Education

    Science.gov (United States)

    Loving, Cathleen C.; Cobern, William W.

    This paper analyzes how Thomas Kuhn's writings are used by others, especially science education researchers. Previous research in citation analysis is used to frame questions related to who cites Kuhn, in what manner and why. Research questions first focus on the variety of disciplines invoking Kuhn and to what extent Structure of Scientific Revolutions (SSR) is cited. The Web of Science database provides material from 1982 for this analysis. The science education literature is analyzed using back issues from 1985 of the Journal of Research in Science Teaching and Science Education. An article analysis reveals trends in terms of what Kuhnian ideas are most frequently invoked. Results indicate a wide array of disciplines from beekeeping to law cite Kuhn - especially generic citations to SSR. The science education journal analysis reveals pervasive use of the term paradigm, although use is quite varied. The two areas of research in science education most impacted by Kuhn appear to be conceptual change theory and constructivist epistemologies. Additional uses of Kuhn are discussed. The degree to which Kuhn is invoked in ways supporting the theoretical framework of citation analysis, whether his work is misappropriated, and the impact of Kuhn are discussed.

  16. Analysis of delayed convergence in the three-noded Timoshenko beam element using the function space approach

    Indian Academy of Sciences (India)

    Somenath Mukherjee; Gangan Prathap

    2002-10-01

    Despite satisfying only completeness and continuity requirements, elements often perform erroneously in a certain class of problems, called the locking situations, where they display spurious stress oscillations and enhanced stiffness properties. The function space approach that effectively substantiates the postulates of the field consistency paradigm is an efficient tool to reveal the fundamental cause of locking phenomena, and propose methods to eliminate this pathological problem. In this paper, we review the delayed convergence behaviour of three-noded Timoshenko beam elements using the rigorous function space approach. Explicit, closed form algebraic results for the element strains, stresses and errors have been derived using this method. The performance of the field-inconsistent three-noded Timoshenko beam element is compared with that of the field-inconsistent twonoded beam element. It is demonstrated that while the field-inconsistent two-noded linear element is prone to shear locking, the field-inconsistent three-noded element is not very vulnerable to this pathological problem, despite the resulting shear oscillations.

  17. WEAK CONVERGENCE OF SOME SERIES

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    This paper continues the study of [1] on weak functions.The weak convergence theory is investigated in complex analysis,Fourier transform and Mellin transform.A Mobius inverse formula of weak functions is obtained.

  18. On the convergence of nanotechnology and Big Data analysis for computer-aided diagnosis.

    Science.gov (United States)

    Rodrigues, Jose F; Paulovich, Fernando V; de Oliveira, Maria Cf; de Oliveira, Osvaldo N

    2016-04-01

    An overview is provided of the challenges involved in building computer-aided diagnosis systems capable of precise medical diagnostics based on integration and interpretation of data from different sources and formats. The availability of massive amounts of data and computational methods associated with the Big Data paradigm has brought hope that such systems may soon be available in routine clinical practices, which is not the case today. We focus on visual and machine learning analysis of medical data acquired with varied nanotech-based techniques and on methods for Big Data infrastructure. Because diagnosis is essentially a classification task, we address the machine learning techniques with supervised and unsupervised classification, making a critical assessment of the progress already made in the medical field and the prospects for the near future. We also advocate that successful computer-aided diagnosis requires a merge of methods and concepts from nanotechnology and Big Data analysis.

  19. A network analysis of the individual – opportunity nexus: Convergence in entrepreneurship research?

    DEFF Research Database (Denmark)

    Thrane, Claus; Blenker, Per

    This paper analyses the citation pattern around the single most cited article in the entrepreneurship discipline in the last decade – ’The promise of entrepreneurship as a field of resaerch’ by Shane & Venkataraman (2000).  Using a quantitative network analysis five clusters pertaining to differe......-opportunity nexus as the defining aspect of entrepreneurship research, but it has produced fundamental disagreement concerning the particular content of this nexus.......This paper analyses the citation pattern around the single most cited article in the entrepreneurship discipline in the last decade – ’The promise of entrepreneurship as a field of resaerch’ by Shane & Venkataraman (2000).  Using a quantitative network analysis five clusters pertaining to different...... that two of these clusters are relatively more central in the citation network: 1) A cluster associated with aspects of individual cognition and entrepreneurial opportunities, and 2) A cluster associated with meta-theoretical aspects of the entrepreneurship discipline. The final part of the paper performs...

  20. Management, Analysis, and Visualization of Experimental and Observational Data -- The Convergence of Data and Computing

    Energy Technology Data Exchange (ETDEWEB)

    Bethel, E. Wes; Greenwald, Martin; Kleese van Dam, Kersten; Parashar, Manish; Wild, Stefan, M.; Wiley, H. Steven

    2016-10-27

    Scientific user facilities---particle accelerators, telescopes, colliders, supercomputers, light sources, sequencing facilities, and more---operated by the U.S. Department of Energy (DOE) Office of Science (SC) generate ever increasing volumes of data at unprecedented rates from experiments, observations, and simulations. At the same time there is a growing community of experimentalists that require real-time data analysis feedback, to enable them to steer their complex experimental instruments to optimized scientific outcomes and new discoveries. Recent efforts in DOE-SC have focused on articulating the data-centric challenges and opportunities facing these science communities. Key challenges include difficulties coping with data size, rate, and complexity in the context of both real-time and post-experiment data analysis and interpretation. Solutions will require algorithmic and mathematical advances, as well as hardware and software infrastructures that adequately support data-intensive scientific workloads. This paper presents the summary findings of a workshop held by DOE-SC in September 2015, convened to identify the major challenges and the research that is needed to meet those challenges.

  1. 浅析网络融合与数字媒体集成%Analysis of network convergence and digital media integration

    Institute of Scientific and Technical Information of China (English)

    孙君菊; 曾雪松

    2014-01-01

    通过对网络融合与数字媒体集成的社会背景分析、融合目标探究以及社会价值评估,探讨网络融合与数字媒体集成发展的必然性。%Based on network convergence and digital media integration analysis of social background , fusion target explorationand social value assessment,we discusses the inevitability of network convergence and digital media integration development.

  2. Systematic toxicological analysis revealing a rare case of captan ingestion.

    Science.gov (United States)

    Gottzein, Anne K; Musshoff, Frank; Madea, Burkhard

    2013-07-01

    This article presents a case of suicide by intoxication with various pharmaceuticals, particularly anticonvulsants, combined with the fungicide captan. A cause of death could not be ascertained at autopsy. However, systematic toxicological analysis (STA) including a screening via solid-phase microextraction (SPME) and gas chromatography-mass spectrometry (GC-MS) for (semi) volatile organic compounds revealed results suggesting a possible cause of death. The effects of captan on the human organism, its metabolism, and distribution will be discussed. Macroscopically, the cause of death was unascertained. STA revealed clonazepam, citalopram, and its metabolites, lamotrigine, levetiracetam, lacosamide, clonazepam, captan, and its metabolite tetrahydrophthalimide (THPI). For the first time, it was detected in human viscera. A quantification of THPI was performed to obtain distribution in the organs. The significance of a complete STA must be emphasized. The presence of THPI would have been missed without previous detection of captan. Consequently, this fatality would not have been investigated satisfactorily.

  3. Convergence of barycentric coordinates to barycentric kernels

    KAUST Repository

    Kosinka, Jiří

    2016-02-12

    We investigate the close correspondence between barycentric coordinates and barycentric kernels from the point of view of the limit process when finer and finer polygons converge to a smooth convex domain. We show that any barycentric kernel is the limit of a set of barycentric coordinates and prove that the convergence rate is quadratic. Our convergence analysis extends naturally to barycentric interpolants and mappings induced by barycentric coordinates and kernels. We verify our theoretical convergence results numerically on several examples.

  4. Low-Dose, Low-Temperature Convergent-Beam Electron Diffraction and Multiwavelength Analysis of Hydrocarbon Films by Electron Diffraction

    Science.gov (United States)

    Wu, Jinsong; Spence, John C. H.

    2003-10-01

    Aromatic hydrocarbon (perylene, coronene) and tetracontane films are shown to produce useful convergent-beam electron diffraction (CBED) patterns under low-dose and low-temperature conditions. These were obtained using a Zeiss LEO-921 electron microscope with an omega energy filter at liquid helium and nitrogen temperatures. The usefulness of patterns showing CBED disks of constant intensity (“blank disks,” indicating kinematic scattering) for structure analysis is investigated, with the aim of avoiding film-bending artifacts. Using CBED patterns from thicker areas, sample thickness was experimentally determined using either two-beam or three-beam patterns. Koehler mode illumination (a new form of SAD pattern offering smaller areas) was also used, and the possibility of obtaining structure factor moduli using the kinematic and two-beam approximations was investigated by comparing measured diffraction intensities with experimental ones for these known structures. The commonly used approximation |F| [similar] Ig (intended to account for bending) was found to be a worse approximation than the two-beam approximation with well-defined excitation error for these microdiffraction experiments. A new multiwavelength method of retrieving structure factor moduli and thickness from microdiffraction patterns using two-beam theory is demonstrated for tetracontane.

  5. Simultaneous analysis of eight vitamin E isomers in Moringa oleifera Lam. leaves by ultra performance convergence chromatography.

    Science.gov (United States)

    Qi, Ningli; Gong, Xiao; Feng, Cuiping; Wang, Xiaoxi; Xu, Yongwei; Lin, Lijing

    2016-09-15

    A new method for simultaneous determination of eight vitamin E isomers including α-, β-, γ-, and δ-tocopherol and α-, β-, γ-, and δ-tocotrienol by ultra-performance convergence chromatography (UPC(2)) equipped with a diode array detector was reported. They were separated on a BEH 2-EP column (3.0 mm × 100 mm, 1.7 μm) using gradient elution (95:5-80:20) with a mobile phase consisted of CO2 and methanol:isopropanol (1:1, v/v), back pressure of 1800 psi, flow rate of 1.5 ml/min and detection at 294 nm. The results showed good linearity (R(2)=0.9990-0.9998) and high resolution (1.48-7.67). Limits of detection (LOD) and quantification (LOQ) ranged from 23-49 ng/L and 70-150 ng/L, respectively. Relative standard deviations (RSD) for repeatability and reproducibility were 0.62-3.16% and 0.82-3.34%, respectively. Moreover, this method was successfully applied to analysis the vitamin E isomers in Moringa oleifera leaf samples.

  6. Nonlinear functional connectivity network recovery in the human brain with mutual connectivity analysis (MCA): convergent cross-mapping and non-metric clustering

    Science.gov (United States)

    Wismüller, Axel; Abidin, Anas Z.; D'Souza, Adora M.; Wang, Xixi; Hobbs, Susan K.; Leistritz, Lutz; Nagarajan, Mahesh B.

    2015-03-01

    We explore a computational framework for functional connectivity analysis in resting-state functional MRI (fMRI) data acquired from the human brain for recovering the underlying network structure and understanding causality between network components. Termed mutual connectivity analysis (MCA), this framework involves two steps, the first of which is to evaluate the pair-wise cross-prediction performance between fMRI pixel time series within the brain. In a second step, the underlying network structure is subsequently recovered from the affinity matrix using non-metric network clustering approaches, such as the so-called Louvain method. Finally, we use convergent cross-mapping (CCM) to study causality between different network components. We demonstrate our MCA framework in the problem of recovering the motor cortex network associated with hand movement from resting state fMRI data. Results are compared with a ground truth of active motor cortex regions as identified by a task-based fMRI sequence involving a finger-tapping stimulation experiment. Our results regarding causation between regions of the motor cortex revealed a significant directional variability and were not readily interpretable in a consistent manner across subjects. However, our results on whole-slice fMRI analysis demonstrate that MCA-based model-free recovery of regions associated with the primary motor cortex and supplementary motor area are in close agreement with localization of similar regions achieved with a task-based fMRI acquisition. Thus, we conclude that our MCA methodology can extract and visualize valuable information concerning the underlying network structure between different regions of the brain in resting state fMRI.

  7. Stability of spherical converging shock wave

    Energy Technology Data Exchange (ETDEWEB)

    Murakami, M., E-mail: murakami-m@ile.osaka-u.ac.jp [Institute of Laser Engineering, Osaka University, Osaka 565-0871 (Japan); Sanz, J. [ETSI Aeronauticos, Universidad Politecnica de Madrid, Madrid 28040 (Spain); Iwamoto, Y. [Graduate School of Science and Engineering, Ehime University, Ehime 790-8577 (Japan)

    2015-07-15

    Based on Guderley's self-similar solution, stability of spherical converging shock wave is studied. A rigorous linear perturbation theory is developed, in which the growth rate of perturbation is given as a function of the spherical harmonic number ℓ and the specific heats ratio γ. Numerical calculation reveals the existence of a γ-dependent cut-off mode number ℓ{sub c}, such that all the eigenmode perturbations for ℓ > ℓ{sub c} are smeared out as the shock wave converges at the center. The analysis is applied to partially spherical geometries to give significant implication for different ignition schemes of inertial confinement fusion. Two-dimensional hydrodynamic simulations are performed to verify the theory.

  8. BRAZILIAN AND INTERNATIONAL ACCOUNTING STANDARDS APPLIED TO THE PUBLIC SECTOR AND THE CHALLENGE OF CONVERGENCE: A COMPARATIVE ANALYSIS - IPSAS AND NBCTSP

    Directory of Open Access Journals (Sweden)

    José Francisco Ribeiro Filho (in memoriam

    2012-11-01

    Full Text Available The aim in this study is to analyze the current stage of conceptual convergence between Brazilian accounting standards applied to the public sector (NBCTSP and the International Public Sector Accounting Standard (IPSAS. The complexity and range of transactions between public or private sector entities, as a result of market internationalization, demand continuous and dynamic assessment of the events that promote quantitative or qualitative equity changes. For this evaluation process, observing accounting principles and standards is important to guarantee, among other information characteristics, understandability and comparability, thus reducing costs for investors and users in general, in view of the barriers raised by diverse languages, cultures, tax and economic policies. For convergence analysis, the standards’ contents were subject to a comparative study, based on a descriptive analysis, with a view to verifying the existing adherence between Brazilian and international standards applied to the public sector. The results found highlight that different aspects still have to be discussed with a view to an actual convergence with the international standards; the current convergence is partial. The high-quality conceptual exposure of the NBCPSPs is observed though, while the contents of the IPSAS are more focused on operating procedures

  9. Modernization, globalization and the welfare state: a comparative analysis of old and new convergence of social insurance since 1930.

    Science.gov (United States)

    Montanari, I

    2001-09-01

    Convergence of policies and institutions across countries has been a recurrent theme within social sciences. 'Old' and 'new' convergence hypotheses have been associated with changing concepts and catchwords, such as modernization, logic of industrialism, post-industrialism, post-Fordism and globalization, but share some underlying theoretical perspectives. The purpose of this paper is to analyse tendencies towards convergence of social insurance systems in 18 OECD countries between 1930 and 1990, a period which has seen our sample of countries develop from predominantly agricultural societies to industrial or post-industrial market democracies. Data from the Social Citizenship Indicator Program (SCIP) are used to examine the development of institutional variables within the various national social insurance systems. Sub-samples of larger and smaller countries are examined separately, in order to test the open-economy hypothesis that smaller countries, being more exposed to international pressures than larger ones, could be expected to show higher degrees of social protection and also more convergence. Hypotheses on differentiated institutional barriers against pressures from the processes of transnationalization of the economy, as well as possible convergence effects of the supra-national policy making within the European Union, are discussed in the last section.

  10. Fast Principal-Component Analysis Reveals Convergent Evolution of ADH1B in Europe and East Asia

    Science.gov (United States)

    Galinsky, Kevin J.; Bhatia, Gaurav; Loh, Po-Ru; Georgiev, Stoyan; Mukherjee, Sayan; Patterson, Nick J.; Price, Alkes L.

    2016-01-01

    Searching for genetic variants with unusual differentiation between subpopulations is an established approach for identifying signals of natural selection. However, existing methods generally require discrete subpopulations. We introduce a method that infers selection using principal components (PCs) by identifying variants whose differentiation along top PCs is significantly greater than the null distribution of genetic drift. To enable the application of this method to large datasets, we developed the FastPCA software, which employs recent advances in random matrix theory to accurately approximate top PCs while reducing time and memory cost from quadratic to linear in the number of individuals, a computational improvement of many orders of magnitude. We apply FastPCA to a cohort of 54,734 European Americans, identifying 5 distinct subpopulations spanning the top 4 PCs. Using the PC-based test for natural selection, we replicate previously known selected loci and identify three new genome-wide significant signals of selection, including selection in Europeans at ADH1B. The coding variant rs1229984∗T has previously been associated to a decreased risk of alcoholism and shown to be under selection in East Asians; we show that it is a rare example of independent evolution on two continents. We also detect selection signals at IGFBP3 and IGH, which have also previously been associated to human disease. PMID:26924531

  11. Convergent Validity of the PUTS

    Directory of Open Access Journals (Sweden)

    Valerie Cathérine Brandt

    2016-04-01

    Full Text Available Premonitory urges are a cardinal feature in Gilles de la Tourette syndrome. Severity of premonitory urges can be assessed with the Premonitory Urge for Tic Disorders Scale (PUTS. However, convergent validity of the measure has been difficult to assess due to the lack of other urge measures.We investigated the relationship between average real-time urge intensity assessed by an in-house developed real-time urge monitor, measuring urge intensity continuously for 5mins on a visual analogue scale, and general urge intensity assessed by the PUTS in 22 adult Tourette patients (mean age 29.8+/- 10.3; 19 male. Additionally, underlying factors of premonitory urges assessed by the PUTS were investigated in the adult sample using factor analysis and were replicated in 40 children and adolescents diagnosed with Tourette syndrome (mean age 12.05 +/- 2.83 SD, 31 male.Cronbach’s alpha for the PUTS10 was acceptable (α = .79 in the adult sample. Convergent validity between average real-time urge intensity scores (as assessed with the real-time urge monitor and the 10-item version of the PUTS (r = .64 and the 9-item version of the PUTS (r = .66 was good. A factor analysis including the 10 items of the PUTS and average real-time urge intensity scores revealed three factors. One factor included the average real-time urge intensity score and appeared to measure urge intensity, while the other two factors can be assumed to reflect the (sensory quality of urges and subjective control, respectively. The factor structure of the 10 PUTS items alone was replicated in a sample of children and adolescents.The results indicate that convergent validity between the PUTS and the real-time urge assessment monitor is good. Furthermore, the results suggest that the PUTS might assess more than one dimension of urges and it may be worthwhile developing different sub-scales of the PUTS assessing premonitory urges in terms of intensity and quality, as well as subjectively

  12. Strong path convergence from Loewner driving convergence

    CERN Document Server

    Sheffield, Scott

    2010-01-01

    We show that, under mild assumptions on the limiting curve, a sequence of simple chordal planar curves converges uniformly whenever certain Loewner driving functions converge. We extend this result to random curves. The random version applies in particular to random lattice paths that have chordal SLE as a scaling limit, with kappa less than 8 (non-space-filling). Existing SLE convergence proofs often begin by showing that the Loewner driving functions of these paths (viewed from infinity) converge to Brownian motion. Unfortunately, this is not sufficient, and additional arguments are required to complete the proofs. We show that driving function convergence is sufficient if it can be established for both parametrization directions and a generic target.

  13. Trophic convergence drives morphological convergence in marine tetrapods.

    Science.gov (United States)

    Kelley, Neil P; Motani, Ryosuke

    2015-01-01

    Marine tetrapod clades (e.g. seals, whales) independently adapted to marine life through the Mesozoic and Caenozoic, and provide iconic examples of convergent evolution. Apparent morphological convergence is often explained as the result of adaptation to similar ecological niches. However, quantitative tests of this hypothesis are uncommon. We use dietary data to classify the feeding ecology of extant marine tetrapods and identify patterns in skull and tooth morphology that discriminate trophic groups across clades. Mapping these patterns onto phylogeny reveals coordinated evolutionary shifts in diet and morphology in different marine tetrapod lineages. Similarities in morphology between species with similar diets-even across large phylogenetic distances-are consistent with previous hypotheses that shared functional constraints drive convergent evolution in marine tetrapods.

  14. Precision Nutrition 4.0: A Big Data and Ethics Foresight Analysis--Convergence of Agrigenomics, Nutrigenomics, Nutriproteomics, and Nutrimetabolomics.

    Science.gov (United States)

    Özdemir, Vural; Kolker, Eugene

    2016-02-01

    , and aggregation of the relevant life sciences data. For innovation in Big Data ethics oversight, we suggest "nested governance" wherein the processes of knowledge production are made transparent in the continuum from life sciences and social sciences to humanities, and where each innovation actor reports to another accountability and transparency layer: scientists to ethicists, and ethicists to scholars in the emerging field of ethics-of-ethics. Such nested innovation ecosystems offer safety against innovation blind spots, calibrate visible/invisible power differences in the cultures of science or ethics, and ultimately, reducing the risk of "paper values"--what people say--and "real values"--what innovation actors actually do. We are optimistic that the convergence of nutrigenomics with nutriproteomics, nutrimetabolomics, and agrigenomics can build a robust, sustainable, and trustworthy precision nutrition 4.0 agenda, as articulated in this Big Data and ethics foresight analysis.

  15. Convergence Analysis of Regional Economic Growth on Central Region in China%我国中部地区经济增长收敛性分析

    Institute of Scientific and Technical Information of China (English)

    张可; 栾贵勘; 刘超

    2009-01-01

    Using the population-weighting of the Theil index of regional economic analysis of the central σ-convergence,found that the central regions were obvious existence of σ-convergence in 1977-2007,and provincial economic disparities were the central σ-convergence of regional economic obstacles factors.At the same time,using the Barro and Sala-I-Martin classic model of the central β-convergence of the regional economy though the impact on economic growth add the dynamic variables--urbanization,industrialization and other conditions were as follows:the central region of the conditions,there was a significant β-convergence,one of industrialization and innovation ability to affect the central region were the conditions of β-convergence of the primary factors,excessive government intervention in the economy that have hindered the conduct of the conditions of β-convergence of the important factors.%运用以人口为权数的Theil指数分析我国中部地区经济σ-收敛发现:1997-2007年中部地区存在较明显的σ-收敛,且省际间的经济差异是中部地区经济σ-收敛的阻碍因素;同时运用Barro和Sala-i-Martin经典模型分析中部地区经济β-收敛,通过加入影响经济增长的动态变量--城市化、工业化等条件发现:中部地区存在显著的条件β-收敛,其中工业化和创新能力是影响中部地区条件β-收敛的首要因素,政府过度干预经济的行为是阻碍其条件β-收敛的重要因素.

  16. Convergence analysis of the Filtered-U LMS algorithm for active noise control in case perfect cancellation is not possible

    NARCIS (Netherlands)

    Fraanje, P.R.; Verhaegen, M.; Doelman, N.J.

    2003-01-01

    The Filtered-U LMS algorithm, proposed by Eriksson for active noise control applications, adapts the coefficients of an infinite-impulse response controller. Conditions for global convergence of the Filtered-U LMS algorithm were presented by Wang and Ren (Signal Processing, 73 (1999) 3) and Mosquera

  17. Interest Convergence or Divergence? A Critical Race Analysis of Asian Americans, Meritocracy, and Critical Mass in the Affirmative Action Debate

    Science.gov (United States)

    Park, Julie J.; Liu, Amy

    2014-01-01

    We use the Critical Race Theory frameworks of interest convergence and divergence to critique the anti-affirmative action movement's co-option of Asian Americans. Past discussions of affirmative action and Asian Americans mainly concentrate on how Asian Americans are affected by affirmative action, whether positively or negatively. We demonstrate…

  18. Value-creation in new product development within converging value chains: An analysis in the functional foods and nutraceutical industry

    NARCIS (Netherlands)

    Bröring, S.; Cloutier, D.

    2008-01-01

    Abstract: Purpose ¿ This paper seeks to shed some light on value-creation in new product development (NPD) projects within the context of industry convergence and to explore alternative types of projects characterised by different buyer-seller relationships. Design/methodology/approach ¿ There has b

  19. Convergence analysis and numerical study of a fixed-point iterative method for solving systems of nonlinear equations.

    Science.gov (United States)

    Huang, Na; Ma, Changfeng

    2014-01-01

    We present a fixed-point iterative method for solving systems of nonlinear equations. The convergence theorem of the proposed method is proved under suitable conditions. In addition, some numerical results are also reported in the paper, which confirm the good theoretical properties of our approach.

  20. A light and electron microscopic analysis of the convergent insular cortical and amygdaloid projections to the posterior lateral hypothalamus in the rat, with special reference to cardiovascular function.

    Science.gov (United States)

    Tsumori, Toshiko; Yokota, Shigefumi; Qin, Yi; Oka, Tatsuro; Yasui, Yukihiko

    2006-11-01

    The synaptic organization between and among the insular cortex (IC) axons, central amygdaloid nucleus (ACe) axons and posterolateral hypothalamus (PLH) neurons was investigated in the rat using double anterograde tracing and anterograde tracing combined with postembedding immunogold analysis. After ipsilateral injections of biotinylated dextran amine (BDA) into the IC and Phaseolus vulgaris-leucoagglutinin (PHA-L) into the ACe, the conspicuous overlapping distribution of BDA-labeled axon terminals and PHA-L-labeled axon terminals was found in the PLH region just medial to the subthalamic nucleus ipsilateral to the injection sites. At the electron microscopic level, approximately two-thirds of the IC terminals made synapses with small-sized dendrites and the rest did with dendritic spines of the PLH neurons, whereas about 79%, 16% and 5% of the ACe terminals established synapses with small- to medium-sized dendrites, somata, and dendritic spines, respectively, of the PLH neurons. In addition, the IC axon terminals contained densely packed round clear vesicles and their synapses were of asymmetrical type. On the other hand, most of the ACe terminals contained not only pleomorphic clear vesicles but also dense-cored vesicles and their synapses were of symmetrical type although some ACe terminals contained densely packed round clear vesicles and formed asymmetrical synapses. Most of the postsynaptic elements received synaptic inputs from the IC or ACe terminals, and some of single postsynaptic elements received convergent synaptic inputs from both sets of terminals. Furthermore, almost all the ACe terminals were revealed to be immunoreactive for gamma-aminobutyric acid (GABA), by using the anterograde BDA tracing technique combined with immunohistochemistry for GABA. The present data suggest that single PLH neurons are under the excitatory influence of the IC and/or inhibitory influence of the ACe in the circuitry involved in the regulation of cardiovascular functions.

  1. There is poverty convergence

    OpenAIRE

    Crespo Cuaresma, Jesus; Klasen, Stephan; Wacker, Konstantin M.

    2016-01-01

    Martin Ravallion ("Why Don't We See Poverty Convergence?" American Economic Review, 102(1): 504-23; 2012) presents evidence against the existence of convergence in global poverty rates despite convergence in household mean income levels and the close linkage between income growth and poverty reduction. We show that this finding is driven by a specification that demands more than simple convergence in poverty headcount rates and assumes a growth elasticity of poverty reduction, which is well-k...

  2. Comparative analysis of the tardigrade feeding apparatus: adaptive convergence and evolutionary pattern of the piercing stylet system

    Directory of Open Access Journals (Sweden)

    Roberto Guidetti

    2013-03-01

    Full Text Available A thorough analysis of the cuticular parts of tardigrade feeding apparatuses was performed in order to provide a more complete understanding of their evolution and their potential homologies with other animal phyla (e.g. Cycloneuralia and Arthropoda. The buccal- pharyngeal apparatuses of eight species belonging to both Eutardigrada and Heterotardigrada were studied using light and scanning electron microscopy. This study supports and completes a previous study on the relationships between form and function in the buccalpharyngeal apparatus of eutardigrades. The common sclerified structures of the tardigrade buccal-pharyngeal apparatus are: a buccal ring connected to a straight buccal tube, a buccal crown, longitudinal thickenings within the pharynx, and a stylet system composed of piercing stylets within stylet coats, and stylet supports. Specifically, heterotardigrades (Echiniscoidea have a narrow buccal tube; long piercing stylets, each with a longitudinal groove, that cross one another before exiting the mouth; pharyngeal bars and secondary longitudinal thickenings within the pharynx. In contrast, eutardigrades have stylets which are shorter than the buccal tube; Parachela have pharyngeal apophyses and placoids within the pharynx, while Apochela lack a buccal crown and cuticular thickenings within the pharynx, the buccal tube is very wide, and the short stylets are associated with triangular-shaped stylet supports. In both classes, when the piercing stylet tips emerge from the mouth to pierce food, the buccal tube opening is almost completely obstructed, which may hinder food uptake. In heterotardigrades, the crossing of the piercing stylets may further decrease food uptake, however this disadvantage may have been reduced in echiniscids by the evolution of a long buccal tube and long stylets able to run more parallel to the buccal tube. In contrast, eutardigrades evolved different strategies. In the order Apochela and in several

  3. Molecular analysis of sourdough reveals Lactobacillus mindensis sp. nov.

    Science.gov (United States)

    Ehrmann, Matthias A; Müller, Martin R A; Vogel, Rudi F

    2003-01-01

    Genotypic fingerprinting to analyse the bacterial flora of an industrial sourdough revealed a coherent group of strains which could not be associated with a valid species. Comparative 16S rDNA sequence analysis showed that these strains formed a homogeneous cluster distinct from their closest relatives, Lactobacillus farciminis, Lactobacillus alimentarius and Lactobacillus kimchii. To characterize them further, physiological (sugar fermentation, formation of DL-lactate, hydrolysis of arginine, growth temperature, CO2 production) and chemotaxonomic properties have been determined. The DNA G +C content was 37.5 0.2 mol%. The peptidoglycan was of the lysine-D-iso-asparagine (L-Lys-D-Asp) type. The strains were homofermentative, Gram-positive, catalase-negative, non-spore-forming, non-motile rods. They were found as a major stable component of a rye flour sourdough fermentation. Physiological, biochemical as well as genotypic data suggested them to be a new species of the genus Lactobacillus. This was confirmed by DNA-DNA hybridization of genomic DNA, and the name Lactobacillus mindensis is proposed. The type strain of this species is DSM 14500T (=LMG 21508T).

  4. Convergence S-compactifications

    Directory of Open Access Journals (Sweden)

    Bernd Losert

    2014-07-01

    Full Text Available Properties of continuous actions on convergence spaces are investigated. The primary focus is the characterization as to when a continuous action on a convergence space can be continuously extended to an action on a compactification of the convergence space. The largest and smallest such compactifications are studied.

  5. Implicit methods for equation-free analysis: convergence results and analysis of emergent waves in microscopic traffic models

    DEFF Research Database (Denmark)

    Marschler, Christian; Sieber, Jan; Berkemer, Rainer;

    2014-01-01

    within an error that is exponentially small with respect to the small parameter measuring time scale separation. Second, we apply this result to the idealized traffic modeling problem of phantom jams generated by cars with uniform behavior on a circular road. The traffic jams are waves that travel slowly...... to equilibria on the macroscopic level in the equation-free setup. The collapse of the traffic jam to the free flow then corresponds to a saddle-node bifurcation of this macroscopic equilibrium. We continue this bifurcation in two parameters using equation-free analysis....... against the direction of traffic. Equation-free analysis enables us to investigate the behavior of the microscopic traffic model on a macroscopic level. The standard deviation of cars' headways is chosen as the macroscopic measure of the underlying dynamics such that traveling wave solutions correspond...

  6. On the Convergence of Biogeography-Based Optimization for Binary Problems

    Directory of Open Access Journals (Sweden)

    Haiping Ma

    2014-01-01

    Full Text Available Biogeography-based optimization (BBO is an evolutionary algorithm inspired by biogeography, which is the study of the migration of species between habitats. A finite Markov chain model of BBO for binary problems was derived in earlier work, and some significant theoretical results were obtained. This paper analyzes the convergence properties of BBO on binary problems based on the previously derived BBO Markov chain model. Analysis reveals that BBO with only migration and mutation never converges to the global optimum. However, BBO with elitism, which maintains the best candidate in the population from one generation to the next, converges to the global optimum. In spite of previously published differences between genetic algorithms (GAs and BBO, this paper shows that the convergence properties of BBO are similar to those of the canonical GA. In addition, the convergence rate estimate of BBO with elitism is obtained in this paper and is confirmed by simulations for some simple representative problems.

  7. Revisiting the Numerical Convergence of Cohesive-Zone Models in Simulating the Delamination of Composite Adhesive Joints by Using the Finite-Element Analysis

    Science.gov (United States)

    Liu, P. F.; Gu, Z. P.; Hu, Z. H.

    2016-11-01

    Delamination is the dominating failure mechanism in composite adhesive joints. A deep insight into the delamination failure mechanism requires advanced numerical methods. Currently, cohesive-zone models (CZMs), in combination with the finite-element analysis (FEA), have become powerful tools for modeling the initiation and growth of delaminations in composites. However, ensuring the numerical convergence in the CZMs used for a delamination analysis of three-dimensional (3D) composite structures is always a challenging issue due to the "snap-back" instability in the nonlinear implicit FEA, which arises mainly from the cohesive softening behavior. Based on the midplane interpolation technique, first numerical techniques for implementing 3D bilinear and exponential CZMs by using ABAQUS-UEL (user element subroutine) are developed in this paper. In particular, a viscous regularization by introducing the damping effect into the stiffness equation is used to improve the convergence. Two examples, a single-lap composite joint and a composite skin/stiffener panel under tension, demonstrate the numerical technique developed. Then, the effect of cohesion parameters on the numerical convergence based on the viscous regularization is studied.

  8. Are China and Europe converging?

    Institute of Scientific and Technical Information of China (English)

    Bastianin; Andrea; Cattanceo; Cristina; Markandya; Anil

    2009-01-01

    This analysis aims at giving a flavour of what is happening between the European and Chinese markets with reference to selected energy indicators and economic variables. More precisely the analysis is concerned with the convergence between European countries (EU15 + Norway) and China in terms of both per capita GDP and "sustainability indicators". The variables we refer to as "sustainability indicators" are two: energy intensity (EI) and carbon intensity (CI). The paths of such variables will be investigated both looking at the economy as a whole and by analysing what is happening in three sectors of economic activity, namely industry, agriculture and services. It finds strong evidence of convergence in energy intensity and carbon intensity between the EU and China, with rates fast enough to achieve 90% convergence in a matter of two decades.

  9. Numerical analysis of choked converging nozzle flows with surface roughness and heat flux conditions

    Indian Academy of Sciences (India)

    A Alper Ozalp

    2006-02-01

    Choked converging nozzle flow and heat transfer characteristics are numerically investigated by means of a recent computational model that integrates the axisymmetric continuity, state, momentum and energy equations. To predict the combined effects of nozzle geometry, friction and heat transfer rates, analyses are conducted with sufficiently wide ranges of covergence half angle, surface roughness and heat flux conditions. Numerical findings show that inlet Mach and Nusselt numbers decrease up to 23.1% and 15.8% with surface heat flux and by 15.13% and 4.8% due to surface roughness. Considering each convergence half angle case individually results in a linear relation between nozzle discharge coefficients and exit Reynolds numbers with similar slopes. Heat flux implementation, by decreasing the shear stress values, lowers the risks due to wear hazards at upstream sections of flow walls; however the final 10% downstream nozzle portion is determined to be quite critical, where shear stress attains the highest magnitudes. Heat transfer rates are seen to increase in the streamwise direction up to 2.7 times; however high convergence half angles, heat flux and surface roughness conditions lower inlet Nusselt numbers by 70%, 15.8% and 4.8% respectively.

  10. Isotope analysis reveals foraging area dichotomy for atlantic leatherback turtles.

    Directory of Open Access Journals (Sweden)

    Stéphane Caut

    Full Text Available BACKGROUND: The leatherback turtle (Dermochelys coriacea has undergone a dramatic decline over the last 25 years, and this is believed to be primarily the result of mortality associated with fisheries bycatch followed by egg and nesting female harvest. Atlantic leatherback turtles undertake long migrations across ocean basins from subtropical and tropical nesting beaches to productive frontal areas. Migration between two nesting seasons can last 2 or 3 years, a time period termed the remigration interval (RI. Recent satellite transmitter data revealed that Atlantic leatherbacks follow two major dispersion patterns after nesting season, through the North Gulf Stream area or more eastward across the North Equatorial Current. However, information on the whole RI is lacking, precluding the accurate identification of feeding areas where conservation measures may need to be applied. METHODOLOGY/PRINCIPAL FINDINGS: Using stable isotopes as dietary tracers we determined the characteristics of feeding grounds of leatherback females nesting in French Guiana. During migration, 3-year RI females differed from 2-year RI females in their isotope values, implying differences in their choice of feeding habitats (offshore vs. more coastal and foraging latitude (North Atlantic vs. West African coasts, respectively. Egg-yolk and blood isotope values are correlated in nesting females, indicating that egg analysis is a useful tool for assessing isotope values in these turtles, including adults when not available. CONCLUSIONS/SIGNIFICANCE: Our results complement previous data on turtle movements during the first year following the nesting season, integrating the diet consumed during the year before nesting. We suggest that the French Guiana leatherback population segregates into two distinct isotopic groupings, and highlight the urgent need to determine the feeding habitats of the turtle in the Atlantic in order to protect this species from incidental take by

  11. Quantitative flux analysis reveals folate-dependent NADPH production

    Science.gov (United States)

    Fan, Jing; Ye, Jiangbin; Kamphorst, Jurre J.; Shlomi, Tomer; Thompson, Craig B.; Rabinowitz, Joshua D.

    2014-06-01

    ATP is the dominant energy source in animals for mechanical and electrical work (for example, muscle contraction or neuronal firing). For chemical work, there is an equally important role for NADPH, which powers redox defence and reductive biosynthesis. The most direct route to produce NADPH from glucose is the oxidative pentose phosphate pathway, with malic enzyme sometimes also important. Although the relative contribution of glycolysis and oxidative phosphorylation to ATP production has been extensively analysed, similar analysis of NADPH metabolism has been lacking. Here we demonstrate the ability to directly track, by liquid chromatography-mass spectrometry, the passage of deuterium from labelled substrates into NADPH, and combine this approach with carbon labelling and mathematical modelling to measure NADPH fluxes. In proliferating cells, the largest contributor to cytosolic NADPH is the oxidative pentose phosphate pathway. Surprisingly, a nearly comparable contribution comes from serine-driven one-carbon metabolism, in which oxidation of methylene tetrahydrofolate to 10-formyl-tetrahydrofolate is coupled to reduction of NADP+ to NADPH. Moreover, tracing of mitochondrial one-carbon metabolism revealed complete oxidation of 10-formyl-tetrahydrofolate to make NADPH. As folate metabolism has not previously been considered an NADPH producer, confirmation of its functional significance was undertaken through knockdown of methylenetetrahydrofolate dehydrogenase (MTHFD) genes. Depletion of either the cytosolic or mitochondrial MTHFD isozyme resulted in decreased cellular NADPH/NADP+ and reduced/oxidized glutathione ratios (GSH/GSSG) and increased cell sensitivity to oxidative stress. Thus, although the importance of folate metabolism for proliferating cells has been long recognized and attributed to its function of producing one-carbon units for nucleic acid synthesis, another crucial function of this pathway is generating reducing power.

  12. Convergence Analysis of Nonconforming Carey Element on Anisotropic Meshes in 3-D%三维各向异性非协调Carey有限元的收敛性分析

    Institute of Scientific and Technical Information of China (English)

    石东洋; 许超

    2006-01-01

    In this paper, the convergence analysis of the famous Carey element in 3-D is studied on anisotropic meshes. The optimal error estimate is obtained based on some novel techniques and approach, which extends its applications.

  13. CONVERGENCE OF REAL WAGES IN RURAL AREAS IN POLAND

    OpenAIRE

    Piotr Adamczyk

    2016-01-01

    The article concerns the spatial diversity of rural areas. The aim of the article is to evaluate whether the process of convergence of real wages in rural areas in Poland can be observed. The author focuses on two concepts of convergence: absolute (unconditional) beta convergence and sigma convergence. The analysis regards rural districts at NUTS-4 level for 2002–2014. It was stated that rural areas in Poland became similar (converge) in terms of real wages what can help reducing the di...

  14. Testing for convergence from the micro-level

    OpenAIRE

    Giorgio Fazio; Davide Piacentino

    2011-01-01

    In the growth literature, researchers are typically concerned with macro convergence. However, to the extent that macro dynamics result from the underlying microeconomic relations, convergence should also be investigated at the micro-level. In this paper, we suggest an approach that allows exploiting large micro panels to test for convergence. Compared to the traditional convergence analysis, this approach allows obtaining, at the same time, β and σ like convergence parameters for both the mi...

  15. Convergence as Organization

    DEFF Research Database (Denmark)

    Harrison, Yannick Nehemiah Antonio; Risager, Bjarke Skærlund

    2016-01-01

    . The article is based on participant observation of the Blockupy event and interviews with a group of Danish activists who also participated. The article constructs sociospatial narrative that unfolds through three different scales of organization: the Blockupy coalition, the participating formal and informal...... organizations, and the activist subject. This narrative explicates the mode of organization as a ‘convergence space’ (cf. Routledge, 2003), with different ‘roots’ and ‘routes’ of organization (cf. Davies, 2012). Thus, through an analysis of the modes of organization constituting this mass protest event......, this article restates the relevance of the concept of organization, which have recently been ignored or understated in favour of master-narratives of networks or the dichotomy of horizontalism and verticality. It concludes by posing a set of questions for further discussion among both activists...

  16. Convergence analysis of artificial bee colony algorithm%人工蜂群算法的收敛性分析

    Institute of Scientific and Technical Information of China (English)

    宁爱平; 张雪英

    2013-01-01

    The convergence of artificial bee colony algorithm is analyzed theoretically by using the stochastic process theory. Some mathematical definitions of artificial bee colony algorithm and one step transition probability of nectar source position are given and the Markov chain model of the algorithm is established. Some properties of the Markov chain are analyzed, and the conclusions that the artificial bee colony state sequence is a finite homogeneous of Markov chain and the state space of artificial bee colony is irreducible are obtained. It is proved that the artificial bee colony algorithm ensures the global convergence as the algorithm meets two assumptions of the random search algorithm for the global convergence.%利用随机过程理论,对人工蜂群算法收敛性进行理论分析,给出人工蜂群算法的一些数学定义和蜜源位置的一步转移概率,建立人工蜂群算法的Markov链模型,分析此Markov链的一些性质,论证了人工蜂群状态序列是有限齐次Markov链,且状态空间是不可约的。结合随机搜索算法的全局收敛准则,证明了人工蜂群算法能够满足随机搜索算法全局收敛的两个假设,保证算法的全局收敛。

  17. Convergence Analysis of Distributed Control for Operation Cost Minimization of Droop Controlled DC Microgrid Based on Multiagent

    DEFF Research Database (Denmark)

    Li, Chendan; Quintero, Juan Carlos Vasquez; Guerrero, Josep M.

    2016-01-01

    In this paper we present a distributed control method for minimizing the operation cost in DC microgrid based on multiagent system. Each agent is autonomous and controls the local converter in a hierarchical way through droop control, voltage scheduling and collective decision making....... The collective decision for the whole system is made by proposed incremental cost consensus, and only nearest-neighbor communication is needed. The convergence characteristics of the consensus algorithm are analyzed considering different communication topologies and control parameters. Case studies verified...... the proposed method by comparing it without traditional methods. The robustness of system is tested under different communication latency and plug and play operation....

  18. Experimental study and 3D CFD analysis on the optimization of throttle angle for a convergent vortex tube

    Science.gov (United States)

    Rafiee, Seyed Ehsan; Sadeghiazad, M. M.

    2016-12-01

    Seven adjustments of convergent-type Vortex Tube (VT) with different throttle angles were applied. The adjustments were made to analyze the influences of such angles on cold and hot temperature drops as well as flow structures inside the VTs. An experimental setup was designed, and tests were performed on different convergent VT configurations at injection pressures ranging from 0.45 to 0.65 MPa. The angles of the throttle valve were arranged between 30° to 90°, and the numbers of injection nozzles ranged between 2 and 6. Laboratory results indicated that the maximum hot and cold temperature drops ranged from 23.24 to 35 K and from 22.87 to 32.88 K, respectively, at four injection nozzles. Results also showed that temperature drop is a function of hot throttle valve angle with the maximum hot and cold temperature drops depending on the angle applied. We used graphs to demonstrate the changes in the cold and hot temperature drops with respect to hot throttle angle values. These values were interpreted and evaluated to determine the optimum angle, which was 60°. The CFD outputs agreed very well with the laboratory results. The proposed CFD results can help future researchers gain good insights into the complicated separation process taking place inside the VTs.

  19. Converged Registries Solution (CRS)

    Data.gov (United States)

    Department of Veterans Affairs — The Converged Registries platform is a hardware and software architecture designed to host individual patient registries and eliminate duplicative development effort...

  20. Protein-protein interaction and pathway analyses of top schizophrenia genes reveal schizophrenia susceptibility genes converge on common molecular networks and enrichment of nucleosome (chromatin) assembly genes in schizophrenia susceptibility loci.

    Science.gov (United States)

    Luo, Xiongjian; Huang, Liang; Jia, Peilin; Li, Ming; Su, Bing; Zhao, Zhongming; Gan, Lin

    2014-01-01

    Recent genome-wide association studies have identified many promising schizophrenia candidate genes and demonstrated that common polygenic variation contributes to schizophrenia risk. However, whether these genes represent perturbations to a common but limited set of underlying molecular processes (pathways) that modulate risk to schizophrenia remains elusive, and it is not known whether these genes converge on common biological pathways (networks) or represent different pathways. In addition, the theoretical and genetic mechanisms underlying the strong genetic heterogeneity of schizophrenia remain largely unknown. Using 4 well-defined data sets that contain top schizophrenia susceptibility genes and applying protein-protein interaction (PPI) network analysis, we investigated the interactions among proteins encoded by top schizophrenia susceptibility genes. We found proteins encoded by top schizophrenia susceptibility genes formed a highly significant interconnected network, and, compared with random networks, these PPI networks are statistically highly significant for both direct connectivity and indirect connectivity. We further validated these results using empirical functional data (transcriptome data from a clinical sample). These highly significant findings indicate that top schizophrenia susceptibility genes encode proteins that significantly directly interacted and formed a densely interconnected network, suggesting perturbations of common underlying molecular processes or pathways that modulate risk to schizophrenia. Our findings that schizophrenia susceptibility genes encode a highly interconnected protein network may also provide a novel explanation for the observed genetic heterogeneity of schizophrenia, ie, mutation in any member of this molecular network will lead to same functional consequences that eventually contribute to risk of schizophrenia.

  1. Convergence of DFP algorithm

    Institute of Scientific and Technical Information of China (English)

    袁亚湘

    1995-01-01

    The DFP method is one of the most famous numerical algorithms for unconstrained optimization. For uniformly convex objective functions convergence properties of the DFP method are studied. Several conditions that can ensure the global convergence of the DFP method are given.

  2. Changes in cod muscle proteins during frozen storage revealed by proteome analysis and multivariate data analysis

    DEFF Research Database (Denmark)

    Kjærsgård, Inger Vibeke Holst; Nørrelykke, M.R.; Jessen, Flemming

    2006-01-01

    Multivariate data analysis has been combined with proteomics to enhance the recovery of information from 2-DE of cod muscle proteins during different storage conditions. Proteins were extracted according to 11 different storage conditions and samples were resolved by 2-DE. Data generated by 2-DE...... was subjected to principal component analysis (PCA) and discriminant partial least squares regression (DPLSR). Applying PCA to 2-DE data revealed the samples to form groups according to frozen storage time, whereas differences due to different storage temperatures or chilled storage in modified atmosphere...... packing did not lead to distinct changes in protein pattern. Applying DPLSR to the 2-DE data enabled the selection of protein spots critical for differentiation between 3 and 6months frozen storage with 12months frozen storage. Some of these protein spots have been identified by MS/MS, revealing myosin...

  3. BAHAMAS: new SNIa analysis reveals inconsistencies with standard cosmology

    CERN Document Server

    Shariff, H; Trotta, R; van Dyk, D A

    2015-01-01

    We present results obtained by applying our BAyesian HierArchical Modeling for the Analysis of Supernova cosmology (BAHAMAS) software package to the 740 spectroscopically confirmed supernovae type Ia (SNIa) from the "Joint Light-curve Analysis" (JLA) dataset. We simultaneously determine cosmological parameters and standardization parameters, including host galaxy mass corrections, residual scatter and object-by-object intrinsic magnitudes. Combining JLA and Planck Cosmic Microwave Background data, we find significant discrepancies in cosmological parameter constraints with respect to the standard analysis: we find Omega_M = 0.399+/-0.027, 2.8\\sigma\\ higher than previously reported and w = -0.910+/-0.045, 1.6\\sigma\\ higher than the standard analysis. We determine the residual scatter to be sigma_res = 0.104+/-0.005. We confirm (at the 95% probability level) the existence of two sub-populations segregated by host galaxy mass, separated at log_{10}(M/M_solar) = 10, differing in mean intrinsic magnitude by 0.055+...

  4. Convergence of Fuzzy Set Sequences

    Institute of Scientific and Technical Information of China (English)

    FENG Yu-hu

    2002-01-01

    There are more than one mode of convergence with respect to the fuzzy set sequences. In this paper,common six modes of convergence and their relationships are discussed. These six modes are convergence in uniform metric D, convergence in separable metric Dp or D*p, 1 ≤ p <∞, convergence in level set, strong convergence in level set and weak convergence. Suitable counterexamples are given. The necessary and sufficient conditions of convergence in uniform metric D are described. Some comme nts on the convergence of LRfuzzy number sequences are represented.

  5. Suite of Benchmark Tests to Conduct Mesh-Convergence Analysis of Nonlinear and Non-constant Coefficient Transport Codes

    Science.gov (United States)

    Zamani, K.; Bombardelli, F. A.

    2014-12-01

    Verification of geophysics codes is imperative to avoid serious academic as well as practical consequences. In case that access to any given source code is not possible, the Method of Manufactured Solution (MMS) cannot be employed in code verification. In contrast, employing the Method of Exact Solution (MES) has several practical advantages. In this research, we first provide four new one-dimensional analytical solutions designed for code verification; these solutions are able to uncover the particular imperfections of the Advection-diffusion-reaction equation, such as nonlinear advection, diffusion or source terms, as well as non-constant coefficient equations. After that, we provide a solution of Burgers' equation in a novel setup. Proposed solutions satisfy the continuity of mass for the ambient flow, which is a crucial factor for coupled hydrodynamics-transport solvers. Then, we use the derived analytical solutions for code verification. To clarify gray-literature issues in the verification of transport codes, we designed a comprehensive test suite to uncover any imperfection in transport solvers via a hierarchical increase in the level of tests' complexity. The test suite includes hundreds of unit tests and system tests to check vis-a-vis the portions of the code. Examples for checking the suite start by testing a simple case of unidirectional advection; then, bidirectional advection and tidal flow and build up to nonlinear cases. We design tests to check nonlinearity in velocity, dispersivity and reactions. The concealing effect of scales (Peclet and Damkohler numbers) on the mesh-convergence study and appropriate remedies are also discussed. For the cases in which the appropriate benchmarks for mesh convergence study are not available, we utilize symmetry. Auxiliary subroutines for automation of the test suite and report generation are designed. All in all, the test package is not only a robust tool for code verification but it also provides comprehensive

  6. Penicillium simile sp. nov. revealed by morphological and phylogenetic analysis.

    Science.gov (United States)

    Davolos, Domenico; Pietrangeli, Biancamaria; Persiani, Anna Maria; Maggi, Oriana

    2012-02-01

    The morphology of three phenetically identical Penicillium isolates, collected from the bioaerosol in a restoration laboratory in Italy, displayed macro- and microscopic characteristics that were similar though not completely ascribable to Penicillium raistrickii. For this reason, a phylogenetic approach based on DNA sequencing analysis was performed to establish both the taxonomic status and the evolutionary relationships of these three peculiar isolates in relation to previously described species of the genus Penicillium. We used four nuclear loci (both rRNA and protein coding genes) that have previously proved useful for the molecular investigation of taxa belonging to the genus Penicillium at various evolutionary levels. The internal transcribed spacer region (ITS1-5.8S-ITS2), domains D1 and D2 of the 28S rDNA, a region of the tubulin beta chain gene (benA) and part of the calmodulin gene (cmd) were amplified by PCR and sequenced. Analysis of the rRNA genes and of the benA and cmd sequence data indicates the presence of three isogenic isolates belonging to a genetically distinct species of the genus Penicillium, here described and named Penicillium simile sp. nov. (ATCC MYA-4591(T)  = CBS 129191(T)). This novel species is phylogenetically different from P. raistrickii and other related species of the genus Penicillium (e.g. Penicillium scabrosum), from which it can be distinguished on the basis of morphological trait analysis.

  7. Convergence: Yea or Nay?

    Science.gov (United States)

    Villano, Matt

    2008-01-01

    Colleges and universities can never be too prepared, whether for physical attacks or data security breaches. A quick data slice of over 7,000 US higher ed institutions, using the Office of Postsecondary Education's Campus Security Data Analysis Cutting Tool Website and cutting across public and private two- and four-year schools, reveals some…

  8. 全球化时代的城市趋同与特色塑造%Analysis of Urban Convergence and Shaping Identity during the Age of Globalization

    Institute of Scientific and Technical Information of China (English)

    刘晓平

    2013-01-01

    以当代全球化理论成果引入城市研究领域来解释普遍的“城市趋同”现象,归纳了全球化时代城镇特性的分类.通过对历史性建筑与城市文脉的反思与澄清,提出了塑造当代城市特色的主观性和包容性的路径,并介绍了城市特色控制的若干实施机制.%By introducing the achievements of contemporary globalization theory into urban study to explain the general phenomena of "urban convergence",this paper shows that the structural factor in society is one of reasons causing the "convergence" of urban space,and gives classification of urban identity for different cognition and treatment.By the reflection and analysis on historic buildings and urban context,it proposes the way to subjectivity and magnanimity,and provides some practical mechanisms for shaping and controlling contemporary urban identity.

  9. Subfield profitability analysis reveals an economic case for cropland diversification

    Science.gov (United States)

    Brandes, E.; McNunn, G. S.; Schulte, L. A.; Bonner, I. J.; Muth, D. J.; Babcock, B. A.; Sharma, B.; Heaton, E. A.

    2016-01-01

    Public agencies and private enterprises increasingly desire to achieve ecosystem service outcomes in agricultural systems, but are limited by perceived conflicts between economic and ecosystem service goals and a lack of tools enabling effective operational management. Here we use Iowa—an agriculturally homogeneous state representative of the Maize Belt—to demonstrate an economic rationale for cropland diversification at the subfield scale. We used a novel computational framework that integrates disparate but publicly available data to map ˜3.3 million unique potential management polygons (9.3 Mha) and reveal subfield opportunities to increase overall field profitability. We analyzed subfield profitability for maize/soybean fields during 2010-2013—four of the most profitable years in recent history—and projected results for 2015. While cropland operating at a loss of US 250 ha-1 or more was negligible between 2010 and 2013 at 18 000-190 000 ha (profitable areas, incorporating conservation management that breaks even (e.g., planting low-input perennials), into low-yielding portions of fields could increase overall cropland profitability by 80%. This approach is applicable to the broader region and differs substantially from the status quo of ‘top-down’ land management for conservation by harnessing private interest to align profitability with the production of ecosystem services.

  10. Comparative Genomic Analysis Reveals Ecological Differentiation in the Genus Carnobacterium

    Science.gov (United States)

    Iskandar, Christelle F.; Borges, Frédéric; Taminiau, Bernard; Daube, Georges; Zagorec, Monique; Remenant, Benoît; Leisner, Jørgen J.; Hansen, Martin A.; Sørensen, Søren J.; Mangavel, Cécile; Cailliez-Grimal, Catherine; Revol-Junelles, Anne-Marie

    2017-01-01

    Lactic acid bacteria (LAB) differ in their ability to colonize food and animal-associated habitats: while some species are specialized and colonize a limited number of habitats, other are generalist and are able to colonize multiple animal-linked habitats. In the current study, Carnobacterium was used as a model genus to elucidate the genetic basis of these colonization differences. Analyses of 16S rRNA gene meta-barcoding data showed that C. maltaromaticum followed by C. divergens are the most prevalent species in foods derived from animals (meat, fish, dairy products), and in the gut. According to phylogenetic analyses, these two animal-adapted species belong to one of two deeply branched lineages. The second lineage contains species isolated from habitats where contact with animal is rare. Genome analyses revealed that members of the animal-adapted lineage harbor a larger secretome than members of the other lineage. The predicted cell-surface proteome is highly diversified in C. maltaromaticum and C. divergens with genes involved in adaptation to the animal milieu such as those encoding biopolymer hydrolytic enzymes, a heme uptake system, and biopolymer-binding adhesins. These species also exhibit genes for gut adaptation and respiration. In contrast, Carnobacterium species belonging to the second lineage encode a poorly diversified cell-surface proteome, lack genes for gut adaptation and are unable to respire. These results shed light on the important genomics traits required for adaptation to animal-linked habitats in generalist Carnobacterium. PMID:28337181

  11. Proteomic Analysis of Hylocereus polyrhizus Reveals Metabolic Pathway Changes

    Directory of Open Access Journals (Sweden)

    Qingzhu Hua

    2016-09-01

    Full Text Available Red dragon fruit or red pitaya (Hylocereus polyrhizus is the only edible fruit that contains betalains. The color of betalains ranges from red and violet to yellow in plants. Betalains may also serve as an important component of health-promoting and disease-preventing functional food. Currently, the biosynthetic and regulatory pathways for betalain production remain to be fully deciphered. In this study, isobaric tags for relative and absolute quantitation (iTRAQ-based proteomic analyses were used to reveal the molecular mechanism of betalain biosynthesis in H. polyrhizus fruits at white and red pulp stages, respectively. A total of 1946 proteins were identified as the differentially expressed between the two samples, and 936 of them were significantly highly expressed at the red pulp stage of H. polyrhizus. RNA-seq and iTRAQ analyses showed that some transcripts and proteins were positively correlated; they belonged to “phenylpropanoid biosynthesis”, “tyrosine metabolism”, “flavonoid biosynthesis”, “ascorbate and aldarate metabolism”, “betalains biosynthesis” and “anthocyanin biosynthesis”. In betalains biosynthesis pathway, several proteins/enzymes such as polyphenol oxidase, CYP76AD3 and 4,5-dihydroxy-phenylalanine (DOPA dioxygenase extradiol-like protein were identified. The present study provides a new insight into the molecular mechanism of the betalain biosynthesis at the posttranscriptional level.

  12. Proteomic Analysis of Hylocereus polyrhizus Reveals Metabolic Pathway Changes

    Science.gov (United States)

    Hua, Qingzhu; Zhou, Qianjun; Gan, Susheng; Wu, Jingyu; Chen, Canbin; Li, Jiaqiang; Ye, Yaoxiong; Zhao, Jietang; Hu, Guibing; Qin, Yonghua

    2016-01-01

    Red dragon fruit or red pitaya (Hylocereus polyrhizus) is the only edible fruit that contains betalains. The color of betalains ranges from red and violet to yellow in plants. Betalains may also serve as an important component of health-promoting and disease-preventing functional food. Currently, the biosynthetic and regulatory pathways for betalain production remain to be fully deciphered. In this study, isobaric tags for relative and absolute quantitation (iTRAQ)-based proteomic analyses were used to reveal the molecular mechanism of betalain biosynthesis in H. polyrhizus fruits at white and red pulp stages, respectively. A total of 1946 proteins were identified as the differentially expressed between the two samples, and 936 of them were significantly highly expressed at the red pulp stage of H. polyrhizus. RNA-seq and iTRAQ analyses showed that some transcripts and proteins were positively correlated; they belonged to “phenylpropanoid biosynthesis”, “tyrosine metabolism”, “flavonoid biosynthesis”, “ascorbate and aldarate metabolism”, “betalains biosynthesis” and “anthocyanin biosynthesis”. In betalains biosynthesis pathway, several proteins/enzymes such as polyphenol oxidase, CYP76AD3 and 4,5-dihydroxy-phenylalanine (DOPA) dioxygenase extradiol-like protein were identified. The present study provides a new insight into the molecular mechanism of the betalain biosynthesis at the posttranscriptional level. PMID:27690004

  13. Analysis of septins across kingdoms reveals orthology and new motifs

    Directory of Open Access Journals (Sweden)

    Malmberg Russell L

    2007-07-01

    Full Text Available Abstract Background Septins are cytoskeletal GTPase proteins first discovered in the fungus Saccharomyces cerevisiae where they organize the septum and link nuclear division with cell division. More recently septins have been found in animals where they are important in processes ranging from actin and microtubule organization to embryonic patterning and where defects in septins have been implicated in human disease. Previous studies suggested that many animal septins fell into independent evolutionary groups, confounding cross-kingdom comparison. Results In the current work, we identified 162 septins from fungi, microsporidia and animals and analyzed their phylogenetic relationships. There was support for five groups of septins with orthology between kingdoms. Group 1 (which includes S. cerevisiae Cdc10p and human Sept9 and Group 2 (which includes S. cerevisiae Cdc3p and human Sept7 contain sequences from fungi and animals. Group 3 (which includes S. cerevisiae Cdc11p and Group 4 (which includes S. cerevisiae Cdc12p contain sequences from fungi and microsporidia. Group 5 (which includes Aspergillus nidulans AspE contains sequences from filamentous fungi. We suggest a modified nomenclature based on these phylogenetic relationships. Comparative sequence alignments revealed septin derivatives of already known G1, G3 and G4 GTPase motifs, four new motifs from two to twelve amino acids long and six conserved single amino acid positions. One of these new motifs is septin-specific and several are group specific. Conclusion Our studies provide an evolutionary history for this important family of proteins and a framework and consistent nomenclature for comparison of septin orthologs across kingdoms.

  14. Modes of embayed beach dynamics: analysis reveals emergent timescales

    Science.gov (United States)

    Murray, K. T.; Murray, A.; Limber, P. W.; Ells, K. D.

    2013-12-01

    Embayed beaches, or beaches positioned between rocky headlands, exhibit morphologic changes over many length and time scales. Beach sediment is transported as a result of the day-to-day wave forcing, causing patterns of erosion and accretion. We use the Rocky Coastline Evolution Model (RCEM) to investigate how patterns of shoreline change depend on wave climate (the distribution of wave-approach angles) and beach characteristics. Measuring changes in beach width through time allows us to track the evolution of the shape of the beach and the movement of sand within it. By using Principle Component Analysis (PCA), these changes can be categorized into modes, where the first few modes explain the majority of the variation in the time series. We analyze these modes and how they vary as a function of wave climate and headland/bay aspect ratio. In the purposefully simple RCEM, sediment transport is wave-driven and affected by wave shadowing behind the headlands. The rock elements in our model experiments (including the headlands) are fixed and unerodable so that this analysis can focus purely on sand dynamics between the headlands, without a sand contribution from the headlands or cliffs behind the beach. The wave climate is characterized by dictating the percentage of offshore waves arriving from the left and the percentage of waves arriving from high angles (very oblique to the coastline orientation). A high-angle dominated wave climate tends to amplify coastline perturbations, whereas a lower-angle wave climate is diffusive. By changing the headland/bay aspect ratio and wave climate, we can perform PCA analysis of generalized embayed beaches with differing anatomy and wave climate forcings. Previous work using PCA analysis of embayed beaches focused on specific locations and shorter timescales (beach dynamics over longer timescales. The first two PCA modes, which explain a majority of the beach width time series variation (typically >70%), are a 'breathing' mode and a

  15. Nonaccommodative convergence excess.

    Science.gov (United States)

    von Noorden, G K; Avilla, C W

    1986-01-15

    Nonaccommodative convergence excess is a condition in which a patient has orthotropia or a small-angle esophoria or esotropia at distance and a large-angle esotropia at near, not significantly reduced by the addition of spherical plus lenses. The AC/A ratio, determined with the gradient method, is normal or subnormal. Tonic convergence is suspected of causing the convergence excess in these patients. Nonaccommodative convergence excess must be distinguished from esotropia with a high AC/A ratio and from hypoaccommodative esotropia. In 24 patients treated with recession of both medial recti muscles with and without posterior fixation or by posterior fixation alone, the mean correction of esotropia was 7.4 prism diopters at distance and 17 prism diopters at near.

  16. Convergent Aeronautics Solutions Project

    Data.gov (United States)

    National Aeronautics and Space Administration — The Convergent Aeronautics Solutions (CAS) Project uses short-duration activities to establish early-stage concept and technology feasibility for high-potential...

  17. Fixed mobile convergence handbook

    CERN Document Server

    Ahson, Syed A

    2010-01-01

    From basic concepts to future directions, this handbook provides technical information on all aspects of fixed-mobile convergence (FMC). The book examines such topics as integrated management architecture, business trends and strategic implications for service providers, personal area networks, mobile controlled handover methods, SIP-based session mobility, and supervisory and notification aggregator service. Case studies are used to illustrate technical and systematic implementation of unified and rationalized internet access by fixed-mobile network convergence. The text examines the technolo

  18. Differential network analysis reveals dysfunctional regulatory networks in gastric carcinogenesis.

    Science.gov (United States)

    Cao, Mu-Shui; Liu, Bing-Ya; Dai, Wen-Tao; Zhou, Wei-Xin; Li, Yi-Xue; Li, Yuan-Yuan

    2015-01-01

    Gastric Carcinoma is one of the most common cancers in the world. A large number of differentially expressed genes have been identified as being associated with gastric cancer progression, however, little is known about the underlying regulatory mechanisms. To address this problem, we developed a differential networking approach that is characterized by including a nascent methodology, differential coexpression analysis (DCEA), and two novel quantitative methods for differential regulation analysis. We first applied DCEA to a gene expression dataset of gastric normal mucosa, adenoma and carcinoma samples to identify gene interconnection changes during cancer progression, based on which we inferred normal, adenoma, and carcinoma-specific gene regulation networks by using linear regression model. It was observed that cancer genes and drug targets were enriched in each network. To investigate the dynamic changes of gene regulation during carcinogenesis, we then designed two quantitative methods to prioritize differentially regulated genes (DRGs) and gene pairs or links (DRLs) between adjacent stages. It was found that known cancer genes and drug targets are significantly higher ranked. The top 4% normal vs. adenoma DRGs (36 genes) and top 6% adenoma vs. carcinoma DRGs (56 genes) proved to be worthy of further investigation to explore their association with gastric cancer. Out of the 16 DRGs involved in two top-10 DRG lists of normal vs. adenoma and adenoma vs. carcinoma comparisons, 15 have been reported to be gastric cancer or cancer related. Based on our inferred differential networking information and known signaling pathways, we generated testable hypotheses on the roles of GATA6, ESRRG and their signaling pathways in gastric carcinogenesis. Compared with established approaches which build genome-scale GRNs, or sub-networks around differentially expressed genes, the present one proved to be better at enriching cancer genes and drug targets, and prioritizing

  19. ROMANIA IN THE CONTEXT OF GLOBALIZATION, REGIONALIZATION AND CONVERGENCE

    Directory of Open Access Journals (Sweden)

    Elena Pelinescu

    2007-04-01

    Full Text Available The economic convergence is an instrument of harmonization in areas with high degree of economic integration. For Romania, it is important to know the degree of fulfillment of the convergence criteria and the impact of the policies which sustain their achievement, because few studies had as object the analysis of the degree of fulfillment in countries like Romania and Bulgaria, which were included in the latest EU extension wave. We use different methods in order to show the cointegration process and the results. The techniques applied to the statistical data regarding the nominal convergence (the consolidated budget deficit as a share of GDP, the share of public debt in GDP, the rate of inflation, the interest rate and the exchange rate have revealed some results which appear in others studies too, namely that Romania integrates in the fixed target Maastricht criteria (the budget deficit as a share of GDP, the share of public debt in GDP and follows a pattern of convergence for the other criteria.

  20. Hereditary hemochromatosis: HFE mutation analysis in Greeks reveals genetic heterogeneity.

    Science.gov (United States)

    Papanikolaou, G; Politou, M; Terpos, E; Fourlemadis, S; Sakellaropoulos, N; Loukopoulos, D

    2000-04-01

    Hereditary hemochromatosis (HH) is common among Caucasians; reported disease frequencies vary from 0.3 to 0.8%. Identification of a candidate HFE gene in 1996 was soon followed by the description of two ancestral mutations, i.e., c.845G-->A (C282Y) and c.187C-->G (H63D). To these was recently added the mutation S65C, which may represent a simple polymorphism. The incidence of HH in Greece is unknown but clinical cases are rare. Also unknown is the carrier frequency of the two mutant alleles. A first estimate of the latter is given in the present report. It is based on data from the genetic analysis of 10 unrelated patients of Greek origin who were referred to our center for genotyping and 158 unselected male blood donors. The allele frequencies for the C282Y and H63D mutations were 0.003 and 0.145, respectively. The C282Y allele was detected in 50% of HH patients. This is considerably lower than the frequencies reported for HH patients in the U.S.A. (82%) and France (91 %) and closer to that reported in Italy (64%). Five patients did not carry any known HFE mutation; three may represent cases of juvenile hemochromatosis, given their early onset with iron overload, hypogonadism, and heart disease. We suggest that genetic heterogeneity is more prominent in Southern Europe. It is also possible that the penetrance of the responsible genes is different across the Mediterranean.

  1. Network analysis reveals distinct clinical syndromes underlying acute mountain sickness.

    Directory of Open Access Journals (Sweden)

    David P Hall

    Full Text Available Acute mountain sickness (AMS is a common problem among visitors at high altitude, and may progress to life-threatening pulmonary and cerebral oedema in a minority of cases. International consensus defines AMS as a constellation of subjective, non-specific symptoms. Specifically, headache, sleep disturbance, fatigue and dizziness are given equal diagnostic weighting. Different pathophysiological mechanisms are now thought to underlie headache and sleep disturbance during acute exposure to high altitude. Hence, these symptoms may not belong together as a single syndrome. Using a novel visual analogue scale (VAS, we sought to undertake a systematic exploration of the symptomatology of AMS using an unbiased, data-driven approach originally designed for analysis of gene expression. Symptom scores were collected from 292 subjects during 1110 subject-days at altitudes between 3650 m and 5200 m on Apex expeditions to Bolivia and Kilimanjaro. Three distinct patterns of symptoms were consistently identified. Although fatigue is a ubiquitous finding, sleep disturbance and headache are each commonly reported without the other. The commonest pattern of symptoms was sleep disturbance and fatigue, with little or no headache. In subjects reporting severe headache, 40% did not report sleep disturbance. Sleep disturbance correlates poorly with other symptoms of AMS (Mean Spearman correlation 0.25. These results challenge the accepted paradigm that AMS is a single disease process and describe at least two distinct syndromes following acute ascent to high altitude. This approach to analysing symptom patterns has potential utility in other clinical syndromes.

  2. Network analysis reveals multiscale controls on streamwater chemistry

    Science.gov (United States)

    McGuire, Kevin J.; Torgersen, Christian E.; Likens, Gene E.; Buso, Donald C.; Lowe, Winsor H.; Bailey, Scott W.

    2014-01-01

    By coupling synoptic data from a basin-wide assessment of streamwater chemistry with network-based geostatistical analysis, we show that spatial processes differentially affect biogeochemical condition and pattern across a headwater stream network. We analyzed a high-resolution dataset consisting of 664 water samples collected every 100 m throughout 32 tributaries in an entire fifth-order stream network. These samples were analyzed for an exhaustive suite of chemical constituents. The fine grain and broad extent of this study design allowed us to quantify spatial patterns over a range of scales by using empirical semivariograms that explicitly incorporated network topology. Here, we show that spatial structure, as determined by the characteristic shape of the semivariograms, differed both among chemical constituents and by spatial relationship (flow-connected, flow-unconnected, or Euclidean). Spatial structure was apparent at either a single scale or at multiple nested scales, suggesting separate processes operating simultaneously within the stream network and surrounding terrestrial landscape. Expected patterns of spatial dependence for flow-connected relationships (e.g., increasing homogeneity with downstream distance) occurred for some chemical constituents (e.g., dissolved organic carbon, sulfate, and aluminum) but not for others (e.g., nitrate, sodium). By comparing semivariograms for the different chemical constituents and spatial relationships, we were able to separate effects on streamwater chemistry of (i) fine-scale versus broad-scale processes and (ii) in-stream processes versus landscape controls. These findings provide insight on the hierarchical scaling of local, longitudinal, and landscape processes that drive biogeochemical patterns in stream networks.

  3. Demographic analysis reveals gradual senescence in the flatworm Macrostomum lignano

    Directory of Open Access Journals (Sweden)

    Braeckman Bart P

    2009-07-01

    Full Text Available Abstract Free-living flatworms ("Turbellaria" are appropriate model organisms to gain better insight into the role of stem cells in ageing and rejuvenation. Ageing research in flatworms is, however, still scarce. This is partly due to culture difficulties and the lack of a complete set of demographic data, including parameters such as median lifespan and age-specific mortality rate. In this paper, we report on the first flatworm survival analysis. We used the species Macrostomum lignano, which is an emerging model for studying the reciprocal influence between stem cells, ageing and rejuvenation. This species has a median lifespan of 205 ± 13 days (average ± standard deviation [SD] and a 90th percentile lifespan of 373 ± 32 days. The maximum lifespan, however, is more than 745 days, and the average survival curve is characterised by a long tail because a small number of individuals lives twice as long as 90% of the population. Similar to earlier observations in a wide range of animals, in M. lignano the age-specific mortality rate increases exponentially, but levels off at the oldest ages. To compare the senescence of M. lignano with that of other ageing models, we determined the mortality rate doubling time, which is 0.20 ± 0.02 years. As a result, we can conclude that M. lignano shows gradual senescence at a rate similar to the vertebrate ageing models Rattus norvegicus and Mus musculus. We argue that M. lignano is a suitable model for ageing and rejuvenation research, and especially for the role of stem cells in these processes, due to its accessible stem cell system and regeneration capacity, and the possibility of combining stem cell studies with demographic analyses.

  4. Stochastic Engine Convergence Diagnostics

    Energy Technology Data Exchange (ETDEWEB)

    Glaser, R

    2001-12-11

    The stochastic engine uses a Markov Chain Monte Carlo (MCMC) sampling device to allow an analyst to construct a reasonable estimate of the state of nature that is consistent with observed data and modeling assumptions. The key engine output is a sample from the posterior distribution, which is the conditional probability distribution of the state of nature, given the data. In applications the state of nature may refer to a complicated, multi-attributed feature like the lithology map of a volume of earth, or to a particular related parameter of interest, say the centroid of the largest contiguous sub-region of specified lithology type. The posterior distribution, which we will call f, can be thought of as the best stochastic description of the state of nature that incorporates all pertinent physical and theoretical models as well as observed data. Characterization of the posterior distribution is the primary goal in the Bayesian statistical paradigm. In applications of the stochastic engine, however, analytical calculation of the posterior distribution is precluded, and only a sample drawn from the distribution is feasible. The engine's MCMC technique, which employs the Metropolis-Hastings algorithm, provides a sample in the form of a sequence (chain) of possible states of nature, x{sup (1)}, x{sup (2)}, ..., x{sup (T)}, .... The sequencing is motivated by consideration of comparative likelihoods of the data. Asymptotic results ensure that the sample ultimately spans the entire posterior distribution and reveals the actual state frequencies that characterize the posterior. In mathematical jargon, the sample is an ergodic Markov chain with stationary distribution f. What this means is that once the chain has gone a sufficient number of steps, T{sub 0}, the (unconditional) distribution of the state, x{sup (T)}, at any step T {ge} T{sub 0} is the same (i.e., is ''stationary''), and is the posterior distribution, f. We call T{sub 0} the &apos

  5. Protein-Protein Interaction and Pathway Analyses of Top Schizophrenia Genes Reveal Schizophrenia Susceptibility Genes Converge on Common Molecular Networks and Enrichment of Nucleosome (Chromatin) Assembly Genes in Schizophrenia Susceptibility Loci

    OpenAIRE

    Luo, Xiongjian; Huang, Liang; Jia, Peilin; Li, Ming; SU, Bing; Zhao, Zhongming; Gan, Lin

    2013-01-01

    Recent genome-wide association studies have identified many promising schizophrenia candidate genes and demonstrated that common polygenic variation contributes to schizophrenia risk. However, whether these genes represent perturbations to a common but limited set of underlying molecular processes (pathways) that modulate risk to schizophrenia remains elusive, and it is not known whether these genes converge on common biological pathways (networks) or represent different pathways. In addition...

  6. Converging towards the future

    Energy Technology Data Exchange (ETDEWEB)

    Gale, R. W.

    1997-05-01

    The likelihood of convergence in the electric power industry in Canada and the United Sates was examined, and the impact of such a development with respect to electricity and natural gas was explored. Based on developments to date, convergence between gas and electric utilities was considered an inevitable step towards the ultimate consolidation of the energy industry as a whole. Characteristics shared by gas and electric utilities were described, and likely developments leading to convergence of the two industries were reviewed. According to this author the competition between the opposing utilities will lead to price wars, loss leaders and other marketing strategies. Prices will be set by supply and demand principles. While users will be able to select customized, money-saving energy solutions, for suppliers, the new utility market will be a Darwinian battle royale, where only the most viable will survive. The end result will be a larger market dominated by a few super players.

  7. On the Fuzzy Convergence

    Directory of Open Access Journals (Sweden)

    Abdul Hameed Q. A. Al-Tai

    2011-01-01

    Full Text Available The aim of this paper is to introduce and study the fuzzy neighborhood, the limit fuzzy number, the convergent fuzzy sequence, the bounded fuzzy sequence, and the Cauchy fuzzy sequence on the base which is adopted by Abdul Hameed (every real number r is replaced by a fuzzy number r¯ (either triangular fuzzy number or singleton fuzzy set (fuzzy point. And then, we will consider that some results respect effect of the upper sequence on the convergent fuzzy sequence, the bounded fuzzy sequence, and the Cauchy fuzzy sequence.

  8. Convergence of Networks

    DEFF Research Database (Denmark)

    Prasad, Ramjee; Ruggieri, Marina

    2008-01-01

    The paper focuses on the revolutionary changes that could characterise the future of networks. Those changes involve many aspects in the conceivement and exploitation of networks: architecture, services, technologies and modeling. The convergence of wired and wireless technologies along...... with the integration of system componennts and the convergence of services (e.g. communications and navigation) are only some of the elements that shape the perpsected mosaic. Authors delineate this vision, highlighting the presence of the space and stratospheric components and the related services as building block...

  9. Peptide Vocabulary Analysis Reveals Ultra-Conservation and Homonymity in Protein Sequences

    Directory of Open Access Journals (Sweden)

    Derek Gatherer

    2007-01-01

    Full Text Available A new algorithm is presented for vocabulary analysis (word detection in texts of human origin. It performs at 60%–70% overall accuracy and greater than 80% accuracy for longer words, and approximately 85% sensitivity on Alice in Wonderland, a considerable improvement on previous methods. When applied to protein sequences, it detects short sequences analogous to words in human texts, i.e. intolerant to changes in spelling (mutation, and relatively contextindependent in their meaning (function. Some of these are homonyms of up to 7 amino acids, which can assume different structures in different proteins. Others are ultra-conserved stretches of up to 18 amino acids within proteins of less than 40% overall identity, reflecting extreme constraint or convergent evolution. Different species are found to have qualitatively different major peptide vocabularies, e.g. some are dominated by large gene families, while others are rich in simple repeats or dominated by internally repetitive proteins. This suggests the possibility of a peptide vocabulary signature, analogous to genome signatures in DNA. Homonyms may be useful in detecting convergent evolution and positive selection in protein evolution. Ultra-conserved words may be useful in identifying structures intolerant to substitution over long periods of evolutionary time.

  10. Convergence Analysis of Semi-Implicit Euler Methods for Solving Stochastic Age-Dependent Capital System with Variable Delays and Random Jump Magnitudes

    Directory of Open Access Journals (Sweden)

    Qinghui Du

    2014-01-01

    Full Text Available We consider semi-implicit Euler methods for stochastic age-dependent capital system with variable delays and random jump magnitudes, and investigate the convergence of the numerical approximation. It is proved that the numerical approximate solutions converge to the analytical solutions in the mean-square sense under given conditions.

  11. The convergence of chaotic integrals

    CERN Document Server

    Bauer, O; Bauer, Oliver; Mainieri, Ronnie

    1995-01-01

    We review the convergence of chaotic integrals computed by Monte Carlo simulation, the trace method, dynamical zeta function, and Fredholm determinant on a simple one-dimensional example: the parabola repeller. There is a dramatic difference in convergence between these approaches. The convergence of the Monte Carlo method follows an inverse power law, whereas the trace method and dynamical zeta function converge exponentially, and the Fredholm determinant converges faster than any exponential.

  12. Convergent Filter Bases

    Directory of Open Access Journals (Sweden)

    Coghetto Roland

    2015-09-01

    Full Text Available We are inspired by the work of Henri Cartan [16], Bourbaki [10] (TG. I Filtres and Claude Wagschal [34]. We define the base of filter, image filter, convergent filter bases, limit filter and the filter base of tails (fr: filtre des sections.

  13. Convergence of Arnoldi method

    Energy Technology Data Exchange (ETDEWEB)

    Nevanlinna, O. [Helsinki Univ. of Technology, Espoo (Finland)

    1994-12-31

    This note summarizes some results on (a monitored version of) the Arnoldi method in Hilbert spaces. The interest in working in infinite dimensional spaces comes partly from the fact that only then can one have meaningful asymptotical statements (which hopefully give some light to the convergence of Arnoldi in large dimensional problems with iteration indices far less than the dimension).

  14. The Convergence Years

    Science.gov (United States)

    Kolodzy, Janet; Grant, August E.; DeMars, Tony R.; Wilkinson, Jeffrey S.

    2014-01-01

    The emergence of the Internet, social media, and digital technologies in the twenty-first century accelerated an evolution in journalism and communication that fit under the broad term of convergence. That evolution changed the relationship between news producers and consumers. It broke down the geographical boundaries in defining our communities,…

  15. Convergent Filter Bases

    OpenAIRE

    Coghetto Roland

    2015-01-01

    We are inspired by the work of Henri Cartan [16], Bourbaki [10] (TG. I Filtres) and Claude Wagschal [34]. We define the base of filter, image filter, convergent filter bases, limit filter and the filter base of tails (fr: filtre des sections).

  16. 3' Untranslated regions mediate transcriptional interference between convergent genes both locally and ectopically in Saccharomyces cerevisiae.

    Science.gov (United States)

    Wang, Luwen; Jiang, Ning; Wang, Lin; Fang, Ou; Leach, Lindsey J; Hu, Xiaohua; Luo, Zewei

    2014-01-01

    Paired sense and antisense (S/AS) genes located in cis represent a structural feature common to the genomes of both prokaryotes and eukaryotes, and produce partially complementary transcripts. We used published genome and transcriptome sequence data and found that over 20% of genes (645 pairs) in the budding yeast Saccharomyces cerevisiae genome are arranged in convergent pairs with overlapping 3'-UTRs. Using published microarray transcriptome data from the standard laboratory strain of S. cerevisiae, our analysis revealed that expression levels of convergent pairs are significantly negatively correlated across a broad range of environments. This implies an important role for convergent genes in the regulation of gene expression, which may compensate for the absence of RNA-dependent mechanisms such as micro RNAs in budding yeast. We selected four representative convergent gene pairs and used expression assays in wild type yeast and its genetically modified strains to explore the underlying patterns of gene expression. Results showed that convergent genes are reciprocally regulated in yeast populations and in single cells, whereby an increase in expression of one gene produces a decrease in the expression of the other, and vice-versa. Time course analysis of the cell cycle illustrated the functional significance of this relationship for the three pairs with relevant functional roles. Furthermore, a series of genetic modifications revealed that the 3'-UTR sequence plays an essential causal role in mediating transcriptional interference, which requires neither the sequence of the open reading frame nor the translation of fully functional proteins. More importantly, transcriptional interference persisted even when one of the convergent genes was expressed ectopically (in trans) and therefore does not depend on the cis arrangement of convergent genes; we conclude that the mechanism of transcriptional interference cannot be explained by the transcriptional collision

  17. 3' Untranslated regions mediate transcriptional interference between convergent genes both locally and ectopically in Saccharomyces cerevisiae.

    Directory of Open Access Journals (Sweden)

    Luwen Wang

    2014-01-01

    Full Text Available Paired sense and antisense (S/AS genes located in cis represent a structural feature common to the genomes of both prokaryotes and eukaryotes, and produce partially complementary transcripts. We used published genome and transcriptome sequence data and found that over 20% of genes (645 pairs in the budding yeast Saccharomyces cerevisiae genome are arranged in convergent pairs with overlapping 3'-UTRs. Using published microarray transcriptome data from the standard laboratory strain of S. cerevisiae, our analysis revealed that expression levels of convergent pairs are significantly negatively correlated across a broad range of environments. This implies an important role for convergent genes in the regulation of gene expression, which may compensate for the absence of RNA-dependent mechanisms such as micro RNAs in budding yeast. We selected four representative convergent gene pairs and used expression assays in wild type yeast and its genetically modified strains to explore the underlying patterns of gene expression. Results showed that convergent genes are reciprocally regulated in yeast populations and in single cells, whereby an increase in expression of one gene produces a decrease in the expression of the other, and vice-versa. Time course analysis of the cell cycle illustrated the functional significance of this relationship for the three pairs with relevant functional roles. Furthermore, a series of genetic modifications revealed that the 3'-UTR sequence plays an essential causal role in mediating transcriptional interference, which requires neither the sequence of the open reading frame nor the translation of fully functional proteins. More importantly, transcriptional interference persisted even when one of the convergent genes was expressed ectopically (in trans and therefore does not depend on the cis arrangement of convergent genes; we conclude that the mechanism of transcriptional interference cannot be explained by the

  18. Bayesian network analysis revealed the connectivity difference of the default mode network from the resting-state to task-state.

    Science.gov (United States)

    Wu, Xia; Yu, Xinyu; Yao, Li; Li, Rui

    2014-01-01

    Functional magnetic resonance imaging (fMRI) studies have converged to reveal the default mode network (DMN), a constellation of regions that display co-activation during resting-state but co-deactivation during attention-demanding tasks in the brain. Here, we employed a Bayesian network (BN) analysis method to construct a directed effective connectivity model of the DMN and compared the organizational architecture and interregional directed connections under both resting-state and task-state. The analysis results indicated that the DMN was consistently organized into two closely interacting subsystems in both resting-state and task-state. The directed connections between DMN regions, however, changed significantly from the resting-state to task-state condition. The results suggest that the DMN intrinsically maintains a relatively stable structure whether at rest or performing tasks but has different information processing mechanisms under varied states.

  19. Convergent study of Ru-ligand interactions through QTAIM, ELF, NBO molecular descriptors and TDDFT analysis of organometallic dyes

    Science.gov (United States)

    Sánchez-Coronilla, Antonio; Sánchez-Márquez, Jesús; Zorrilla, David; Martín, Elisa I.; de los Santos, Desireé M.; Navas, Javier; Fernández-Lorenzo, Concha; Alcántara, Rodrigo; Martín-Calleja, Joaquín

    2014-08-01

    We report a theoretical study of a series of Ru complexes of interest in dye-sensitised solar cells, in organic light-emitting diodes, and in the war against cancer. Other metal centres, such as Cr, Co, Ni, Rh, Pd, and Pt, have been included for comparison purposes. The metal-ligand trends in organometallic chemistry for those compounds are shown synergistically by using three molecular descriptors: quantum theory of atoms in molecules (QTAIM), electron localisation function (ELF) and second-order perturbation theory analysis of the natural bond orbital (NBO). The metal-ligand bond order is addressed through both delocalisation index (DI) of QTAIM and fluctuation index (λ) of ELF. Correlation between DI and λ for Ru-N bond in those complexes is introduced for the first time. Electron transfer and stability was also assessed by the second-order perturbation theory analysis of the NBO. Electron transfer from the lone pair NBO of the ligands toward the antibonding lone pair NBO of the metal plays a relevant role in stabilising the complexes, providing useful insights into understanding the effect of the 'expanded ligand' principle in supramolecular chemistry. Finally, absorption wavelengths associated to the metal-to-ligand charge transfer transitions and the highest occupied molecular orbital (HOMO)--lowest unoccupied molecular orbital (LUMO) characteristics were studied by time-dependent density functional theory.

  20. Morphological and molecular convergences in mammalian phylogenetics.

    Science.gov (United States)

    Zou, Zhengting; Zhang, Jianzhi

    2016-09-02

    Phylogenetic trees reconstructed from molecular sequences are often considered more reliable than those reconstructed from morphological characters, in part because convergent evolution, which confounds phylogenetic reconstruction, is believed to be rarer for molecular sequences than for morphologies. However, neither the validity of this belief nor its underlying cause is known. Here comparing thousands of characters of each type that have been used for inferring the phylogeny of mammals, we find that on average morphological characters indeed experience much more convergences than amino acid sites, but this disparity is explained by fewer states per character rather than an intrinsically higher susceptibility to convergence for morphologies than sequences. We show by computer simulation and actual data analysis that a simple method for identifying and removing convergence-prone characters improves phylogenetic accuracy, potentially enabling, when necessary, the inclusion of morphologies and hence fossils for reliable tree inference.

  1. Testing Convergence Versus History: Convergence Dominates Phenotypic Evolution for over 150 Million Years in Frogs.

    Science.gov (United States)

    Moen, Daniel S; Morlon, Hélène; Wiens, John J

    2016-01-01

    Striking evolutionary convergence can lead to similar sets of species in different locations, such as in cichlid fishes and Anolis lizards, and suggests that evolution can be repeatable and predictable across clades. Yet, most examples of convergence involve relatively small temporal and/or spatial scales. Some authors have speculated that at larger scales (e.g., across continents), differing evolutionary histories will prevent convergence. However, few studies have compared the contrasting roles of convergence and history, and none have done so at large scales. Here we develop a two-part approach to test the scale over which convergence can occur, comparing the relative importance of convergence and history in macroevolution using phylogenetic models of adaptive evolution. We apply this approach to data from morphology, ecology, and phylogeny from 167 species of anuran amphibians (frogs) from 10 local sites across the world, spanning ~160 myr of evolution. Mapping ecology on the phylogeny revealed that similar microhabitat specialists (e.g., aquatic, arboreal) have evolved repeatedly across clades and regions, producing many evolutionary replicates for testing for morphological convergence. By comparing morphological optima for clades and microhabitat types (our first test), we find that convergence associated with microhabitat use dominates frog morphological evolution, producing recurrent ecomorphs that together encompass all sampled species in each community in each region. However, our second test, which examines whether and how much species differ from their inferred optima, shows that convergence is incomplete: that is, phenotypes of most species are still somewhat distant from the estimated optimum for each microhabitat, seemingly because of insufficient time for more complete adaptation (an effect of history). Yet, these effects of history are related to past ecologies, and not clade membership. Overall, our study elucidates the dominant drivers of

  2. Professional Communication and Translation in Convergence

    DEFF Research Database (Denmark)

    Gnecci, Marusca; Maylath, Bruce; Mousten, Birthe;

    2008-01-01

    As translation of technical documents becomes commonplace, and as translation becomes more automated, the roles of translator and technical communicator appear to be converging. This paper examines the trend revealed from recent surveys, and it suggests further research to determine if the trend ...... is likely to continue. The  paper also provides recommendations for academic programs interested in adjusting to the trend....

  3. Free Vibration Analysis of Patch Repaired Plates with a Through Crack by p-Convergent Layerwise Element

    Directory of Open Access Journals (Sweden)

    Jae S. Ahn

    2014-01-01

    Full Text Available The high-order layerwise element models have been used for damaged plates and shells in the presence of singularities such as crack, cutout, and delamination. In this study, the extension of a proposed finite element model has been tested for free vibration analysis of composite laminated systems. For the elements, three-dimensional displacement fields can be captured by layer-by-layer representation. For the elements, higher-order shape functions are derived by combination of one- and two-dimensional shape functions based on higher-order Lobatto shape functions, not using pure higher-order three-dimensional shape functions. The present model can relieve difficulty of aspect ratios in modeling very thin thickness of bonding layer. For verification of the model, natural frequencies and corresponding mode shapes are calculated and then compared with reference values for uncracked and cracked plates. Also, the vibration characteristics of one-sided patch repaired plates with a through internal crack are investigated with respect to variation of crack length, size and thickness of patch, and shear modulus of adhesive, respectively.

  4. Convergence of ecological footprint and emergy analysis as a sustainability indicator of countries: Peru as case study

    Science.gov (United States)

    Siche, Raúl; Pereira, Lucas; Agostinho, Feni; Ortega, Enrique

    2010-10-01

    In the last decade, two scientific tools have been extensively used worldwide to measure the human impact on nature: ecological footprint (EF) and emergy analysis (EA). Papers trying to combine the strong points of EF and EA, and obtain more accurate results have appeared in scientific literature, in which Zhao's et al. (2005) [61] approach is an important one. Unfortunately, some weak points of the original methods still remain on the new approaches proposed. The aim of this present work is to discuss some weak points found in Zhao's approach, trying to overcome them through a new approach called emergetic ecological footprint (EEF). The main difference between Zhao's approach and EEF is that the last one accounted for the internal storage of capital natural in the biocapacity calculation. Besides that, soil loss and water for human consume were considered as additional categories in the footprint calculation. After discussing it through comparisons with other approaches, EEF was used to assess Peru as a case study, resulting in a biocapacity of 51.76 gha capita-1 and a footprint of 12.23 gha capita-1, with 2004 data; that resulted in an ecological surplus of 39.53 gha capita-1. The load capacity factor obtained was 4.23, meaning that Peru can support a population 4.23 times bigger considering the life style of 2004. The main limitations of the EEF are: (i) it is impossible to make comparisons between the biocapacity and footprint for each category; (ii) a need for a handbook with emergy intensity factors with good quality. On the other hand, the main positive points are: (i) its easiness of application in global and national scales; (ii) its final indicators account for all the previous energy (or emergy) used to make something; (iii) internal natural capital storage was accounted for in the biocapacity calculation, which can be a valid step towards the evaluation and assess of services provided by nature.

  5. The Convergent Learning Space:

    DEFF Research Database (Denmark)

    Kjærgaard, Hanne Wacher; Kjeldsen, Lars Peter; Asmussen, Jørgen Bering

    2013-01-01

    is described as well as the theoretical construct and hypotheses surrounding the emergence of the concept in technology-rich classrooms, where students bring their own devices and involve their personal learning spaces and networks. The need for new ways of approaching concepts like choice, learning resources......This paper describes the concept of “The Convergent Learning Space” as it is being explored in an ongoing action research project carried out at undergraduate level in select bachelor programs at a Danish University College. The background nature, design, and beginning of this work in progress......, trajectories of participation etc. calls for new action and new pedagogies by teachers in order to secure alignment between students’ worlds and expectations and aims and plans of the teacher. Action research methods are being used to define and test the constituents and variables of the convergent learning...

  6. The Convergent Learning Space

    DEFF Research Database (Denmark)

    Kjeldsen, Lars Peter; Kjærgaard, Hanne Wacher

    2014-01-01

    in classroom understandings, student behaviour and technological possibilities have created the call for new approaches to teaching, where teachers reconsider both their own roles and the roles of learning resources and classroom frameworks. Equally, freedom of choice and the inclusion of informal learning......The concept of the Convergent Learning Space has been hypothesized and explored in an ongoing action research project carried out at undergraduate level in select bachelor programs at a Danish University College, where classrooms are technology rich and students bring their own devices. The changes...... networks are still more prominently expected by students. Against this backdrop, an action research project has worked with the definition and testing of the hypothesized constituents of the Convergent Learning Space and how it challenges our traditional conceptions of learning spaces. The article...

  7. Convergence Reflects Maturity

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    Competition has been playing a dominant role among telecommunication equipment manufacturers. In 2006, however, merger overwhelmed competition. In the tide of converging, a large number of equipment manufacturers were involved in mergers. At the same time, telecommunication equipment manufacturers, the most innovative community in the world, drive 3G technology fast in line with its technical roadmap. In addition, Chinese equipment manufacturers cut a figure in world-class telecommunication markets, whic...

  8. CONVERGENCE OF REAL WAGES IN RURAL AREAS IN POLAND

    Directory of Open Access Journals (Sweden)

    Piotr Adamczyk

    2016-06-01

    Full Text Available The article concerns the spatial diversity of rural areas. The aim of the article is to evaluate whether the process of convergence of real wages in rural areas in Poland can be observed. The author focuses on two concepts of convergence: absolute (unconditional beta convergence and sigma convergence. The analysis regards rural districts at NUTS-4 level for 2002–2014. It was stated that rural areas in Poland became similar (converge in terms of real wages what can help reducing the diff erences in living standard of inhabitants. An estimated regression function confi rms the existence of absolute beta convergence process. The growth rate of real wages was relatively higher in the districts with lower initial level. Moreover, the research carried out by the author indicates a decreasing dispersion of wages among rural districts. It shows the existence of sigma convergence process.

  9. Integrated analysis of transcript-level regulation of metabolism reveals disease-relevant nodes of the human metabolic network.

    Science.gov (United States)

    Galhardo, Mafalda; Sinkkonen, Lasse; Berninger, Philipp; Lin, Jake; Sauter, Thomas; Heinäniemi, Merja

    2014-02-01

    Metabolic diseases and comorbidities represent an ever-growing epidemic where multiple cell types impact tissue homeostasis. Here, the link between the metabolic and gene regulatory networks was studied through experimental and computational analysis. Integrating gene regulation data with a human metabolic network prompted the establishment of an open-sourced web portal, IDARE (Integrated Data Nodes of Regulation), for visualizing various gene-related data in context of metabolic pathways. Motivated by increasing availability of deep sequencing studies, we obtained ChIP-seq data from widely studied human umbilical vein endothelial cells. Interestingly, we found that association of metabolic genes with multiple transcription factors (TFs) enriched disease-associated genes. To demonstrate further extensions enabled by examining these networks together, constraint-based modeling was applied to data from human preadipocyte differentiation. In parallel, data on gene expression, genome-wide ChIP-seq profiles for peroxisome proliferator-activated receptor (PPAR) γ, CCAAT/enhancer binding protein (CEBP) α, liver X receptor (LXR) and H3K4me3 and microRNA target identification for miR-27a, miR-29a and miR-222 were collected. Disease-relevant key nodes, including mitochondrial glycerol-3-phosphate acyltransferase (GPAM), were exposed from metabolic pathways predicted to change activity by focusing on association with multiple regulators. In both cell types, our analysis reveals the convergence of microRNAs and TFs within the branched chain amino acid (BCAA) metabolic pathway, possibly providing an explanation for its downregulation in obese and diabetic conditions.

  10. IT Convergence and Security 2012

    CERN Document Server

    Chung, Kyung-Yong

    2013-01-01

    The proceedings approaches the subject matter with problems in technical convergence and convergences of security technology. This approach is new because we look at new issues that arise from techniques converging. The general scope of the proceedings content is convergence security and the latest information technology. The intended readership are societies, enterprises, and research institutes, and intended content level is mid- to highly educated personals. The most important features and benefits of the proceedings are the introduction of the most recent information technology and its related ideas, applications and problems related to technology convergence, and its case studies and finally an introduction of converging existing security techniques through convergence security. Overall, through the proceedings, authors will be able to understand the most state of the art information strategies and technologies of convergence security.

  11. Differential proteomic analysis reveals novel links between primary metabolism and antibiotic production in Amycolatopsis balhimycina

    DEFF Research Database (Denmark)

    Gallo, G.; Renzone, G.; Alduina, R.

    2010-01-01

    A differential proteomic analysis, based on 2-DE and MS procedures, was performed on Amycolatopsis balhimycina DSM5908, the actinomycete producing the vancomycin-like antibiotic balhimycin. A comparison of proteomic profiles before and during balhimycin production characterized differentially...... intermediates, were upregulated during antibiotic production. qRT-PCR analysis revealed that 8 out of 14 upregulated genes showed a positive correlation between changes at translational and transcriptional expression level. Furthermore, proteomic analysis of two nonproducing mutants, restricted to a sub...

  12. 自校正对角阵加权信息融合Kalman预报器%Self-tuning Information Fusion Kalman Predictor Weighted by Diagonal Matrices and Its Convergence Analysis

    Institute of Scientific and Technical Information of China (English)

    邓自立; 李春波

    2007-01-01

    For the multisensor systems with unknown noise statistics, using the modern time series analysis method, based on on-line identification of the moving average (MA) innovation models, and based on the solution of the matrix equations for correlation function, estimators of the noise variances are obtained, and under the linear minimum variance optimal information fusion criterion weighted by diagonal matrices, a self-tuning information fusion Kalman predictor is presented, which realizes the self-tuning decoupled fusion Kalman predictors for the state components. Based on the dynamic error system, a new convergence analysis method is presented for self-tuning fuser. A new concept of convergence in a realization is presented, which is weaker than the convergence with probability one. It is strictly proved that if the parameter estimation of the MA innovation models is consistent, then the self-tuning fusion Kalman predictor will converge to the optimal fusion Kalman predictor in a realization, or with probability one, so that it has asymptotic optimality. It can reduce the computational burden, and is suitable for real time applications. A simulation example for a target tracking system shows its effectiveness.

  13. Knowledge Convergence and Collaborative Learning

    Science.gov (United States)

    Jeong, Heisawn; Chi, Michelene T. H.

    2007-01-01

    This paper operationalized the notion of knowledge convergence and assessed quantitatively how much knowledge convergence occurred during collaborative learning. Knowledge convergence was defined as an increase in common knowledge where common knowledge referred to the knowledge that all collaborating partners had. Twenty pairs of college students…

  14. Revisiting world energy intensity convergence for regional differences

    Energy Technology Data Exchange (ETDEWEB)

    Liddle, Brantley [Centre for Strategic Economic Studies, Victoria University, Melbourne, VIC 8001 (Australia)

    2010-10-15

    World convergence in energy intensity is revisited using two new large data sets: a 111-country sample spanning 1971-2006, and a 134-country sample spanning 1990-2006. Both data sets confirm continued convergence. However, the larger data set, which adds the former Soviet Union republics and additional Balkan countries, indicates greater convergence over its more recent time-frame. Further investigation of geographical differences reveals that the OECD and Eurasian countries have shown considerable, continued convergence, while the Sub-Saharan African countries have converged amongst themselves, but at a slower rate than the OECD and Eurasian countries; by contrast, Latin American and Caribbean and Middle East and North African countries have exhibited no convergence to divergence in energy intensity. (author)

  15. Metagenomic analysis of a permafrost microbial community reveals a rapid response to thaw.

    Science.gov (United States)

    Mackelprang, Rachel; Waldrop, Mark P; DeAngelis, Kristen M; David, Maude M; Chavarria, Krystle L; Blazewicz, Steven J; Rubin, Edward M; Jansson, Janet K

    2011-11-06

    Permafrost contains an estimated 1672 Pg carbon (C), an amount roughly equivalent to the total currently contained within land plants and the atmosphere. This reservoir of C is vulnerable to decomposition as rising global temperatures cause the permafrost to thaw. During thaw, trapped organic matter may become more accessible for microbial degradation and result in greenhouse gas emissions. Despite recent advances in the use of molecular tools to study permafrost microbial communities, their response to thaw remains unclear. Here we use deep metagenomic sequencing to determine the impact of thaw on microbial phylogenetic and functional genes, and relate these data to measurements of methane emissions. Metagenomics, the direct sequencing of DNA from the environment, allows the examination of whole biochemical pathways and associated processes, as opposed to individual pieces of the metabolic puzzle. Our metagenome analyses reveal that during transition from a frozen to a thawed state there are rapid shifts in many microbial, phylogenetic and functional gene abundances and pathways. After one week of incubation at 5 °C, permafrost metagenomes converge to be more similar to each other than while they are frozen. We find that multiple genes involved in cycling of C and nitrogen shift rapidly during thaw. We also construct the first draft genome from a complex soil metagenome, which corresponds to a novel methanogen. Methane previously accumulated in permafrost is released during thaw and subsequently consumed by methanotrophic bacteria. Together these data point towards the importance of rapid cycling of methane and nitrogen in thawing permafrost.

  16. Decentralisation and convergence in health among the provinces of Spain (1980-2001).

    Science.gov (United States)

    Montero-Granados, Roberto; Jiménez, Juan de Dios; Martín, José

    2007-03-01

    This study measures the process of convergence in the state of health among the provinces and regions of Spain during 1980-2001 in order to analyse the possible influence of the decentralisation of healthcare management to the regions in this period. Sigma and beta convergence models, traditionally employed in macroeconomics, have been used, taking Life Expectancy at Birth (LEB) and Infant Mortality (IM) as health indicators. The analysis was carried out at two territorial levels: provinces and regions. The results reveal that the process of decentralisation either does not affect convergence or leads to divergence in health. Indeed, in the case of IM the so-called change of role scenario seems to have occurred. Thus, certain provinces with initially poor indicators have improved, overtaking those that were originally in a better position. The final result, however, is of greater dispersion than initially.

  17. EU Internet Law in the Era of Convergence

    DEFF Research Database (Denmark)

    Jakobsen, Søren Sandfeld

    2014-01-01

    An analysis of the demarcation and interplay between EU's Internet, Telecoms and Media regulation in light of the growing convergence......An analysis of the demarcation and interplay between EU's Internet, Telecoms and Media regulation in light of the growing convergence...

  18. Convergence Patterns in Latin America

    DEFF Research Database (Denmark)

    Quiroga, Paola Andrea Barrientos

    Literature on convergence among Latin American countries is still scarce compared to other regions. Moreover, almost none of the research connects convergence to the economic history of Latin America and the usual finding is one speed of convergence. In this paper I analyze 32 countries and 108...... years, more observations than any other study. This long span of data allows me to use economic history to explain, analyze, validate, and understand the results of convergence patterns in the region. I find more than one speed of convergence (clubs) related to the known historical background, country...

  19. Understanding the Dynamic Convergence Phenomenon from the Perspective of Diversity and Persistence: A Cross-Sector Comparative Analysis between the United States and South Korea.

    Directory of Open Access Journals (Sweden)

    We Shim

    Full Text Available This study was designed to improve the explanation for the behavior of the phenomenon of technology convergence. The concepts and measurements of diversity and persistence, as inherent attributes of the phenomenon, were elaborated by reviewing different theories. Diversity was examined by analyzing the degree of capability to absorb heterogeneous technologies, while persistence was investigated by analyzing the degree of continuity in the usage of cumulated technologies. With these two dimensions, an analytic framework was proposed to compare the differences and dynamic patterns of convergence competence by countries at the technology sector level. Three major technology sectors in the United States and South Korea, namely, information and communication technology, biotechnology, and nanotechnology, were explored to explicitly illustrate the differences in technology convergence competence. The results show that although Korea has narrowed the differences of capabilities for technology convergence compared to the US, Korea not only has to continuously pursue the improvement of specialization for all three sectors, but also has to encourage the exploitation of different technology fields. The suggested framework and indicators allow for monitoring of the dynamic patterns of a technology sector and identifying the sources of the gaps. Thus, the framework and indicators are able to ensure the purpose of government innovation policy and to provide strategic directions for redistributing the proper combination of sources to accomplish technology convergence.

  20. Medialogy - convergence and transdisciplinarity

    DEFF Research Database (Denmark)

    Nordahl, Rolf

    2007-01-01

    for changes in society, developments in taste etc.  However, it certainly seems fair to say, that available technology makes a great difference to the development of any art form or practice. With the up rise of new educations such as Medialogy, new aspects of convergence and different forms...... of interdisciplinarity and transdisciplinarity is a pre-requisite for both researchers and students. In this talk we will demonstrate our approach through concrete examples of student productions and projects. We will also display the pedagogical method (problem based learning), that enables students to bridge gaps...... between art and science....

  1. Transcriptome analysis reveals key differentially expressed genes involved in wheat grain development

    Directory of Open Access Journals (Sweden)

    Yonglong Yu

    2016-04-01

    Full Text Available Wheat seed development is an important physiological process of seed maturation and directly affects wheat yield and quality. In this study, we performed dynamic transcriptome microarray analysis of an elite Chinese bread wheat cultivar (Jimai 20 during grain development using the GeneChip Wheat Genome Array. Grain morphology and scanning electron microscope observations showed that the period of 11–15 days post-anthesis (DPA was a key stage for the synthesis and accumulation of seed starch. Genome-wide transcriptional profiling and significance analysis of microarrays revealed that the period from 11 to 15 DPA was more important than the 15–20 DPA stage for the synthesis and accumulation of nutritive reserves. Series test of cluster analysis of differential genes revealed five statistically significant gene expression profiles. Gene ontology annotation and enrichment analysis gave further information about differentially expressed genes, and MapMan analysis revealed expression changes within functional groups during seed development. Metabolic pathway network analysis showed that major and minor metabolic pathways regulate one another to ensure regular seed development and nutritive reserve accumulation. We performed gene co-expression network analysis to identify genes that play vital roles in seed development and identified several key genes involved in important metabolic pathways. The transcriptional expression of eight key genes involved in starch and protein synthesis and stress defense was further validated by qRT-PCR. Our results provide new insight into the molecular mechanisms of wheat seed development and the determinants of yield and quality.

  2. Transcriptome analysis reveals key differentially expressed genes involved in wheat grain development

    Institute of Scientific and Technical Information of China (English)

    Yonglong Yu; Dong Zhu; Chaoying Ma; Hui Cao; Yaping Wang; Yanhao Xu; Wenying Zhang; Yueming Yan

    2016-01-01

    Wheat seed development is an important physiological process of seed maturation and directly affects wheat yield and quality. In this study, we performed dynamic transcriptome microarray analysis of an elite Chinese bread wheat cultivar (Jimai 20) during grain development using the GeneChip Wheat Genome Array. Grain morphology and scanning electron microscope observations showed that the period of 11–15 days post-anthesis (DPA) was a key stage for the synthesis and accumulation of seed starch. Genome-wide transcriptional profiling and significance analysis of microarrays revealed that the period from 11 to 15 DPA was more important than the 15–20 DPA stage for the synthesis and accumulation of nutritive reserves. Series test of cluster analysis of differential genes revealed five statistically significant gene expression profiles. Gene ontology annotation and enrichment analysis gave further informa-tion about differentially expressed genes, and MapMan analysis revealed expression changes within functional groups during seed development. Metabolic pathway network analysis showed that major and minor metabolic pathways regulate one another to ensure regular seed development and nutritive reserve accumulation. We performed gene co-expression network analysis to identify genes that play vital roles in seed development and identified several key genes involved in important metabolic pathways. The transcriptional expression of eight key genes involved in starch and protein synthesis and stress defense was further validated by qRT-PCR. Our results provide new insight into the molecular mechanisms of wheat seed development and the determinants of yield and quality.

  3. Using team cognitive work analysis to reveal healthcare team interactions in a birthing unit

    OpenAIRE

    Ashoori, Maryam; Burns, Catherine M.; d'Entremont, Barbara; Momtahan, Kathryn

    2014-01-01

    Cognitive work analysis (CWA) as an analytical approach for examining complex sociotechnical systems has shown success in modelling the work of single operators. The CWA approach incorporates social and team interactions, but a more explicit analysis of team aspects can reveal more information for systems design. In this paper, Team CWA is explored to understand teamwork within a birthing unit at a hospital. Team CWA models are derived from theories and models of teamworkand leverage the exis...

  4. Mosaic Convergence of Rodent Dentitions

    Science.gov (United States)

    Lazzari, Vincent; Charles, Cyril; Tafforeau, Paul; Vianey-Liaud, Monique; Aguilar, Jean-Pierre; Jaeger, Jean-Jacques; Michaux, Jacques; Viriot, Laurent

    2008-01-01

    Background Understanding mechanisms responsible for changes in tooth morphology in the course of evolution is an area of investigation common to both paleontology and developmental biology. Detailed analyses of molar tooth crown shape have shown frequent homoplasia in mammalian evolution, which requires accurate investigation of the evolutionary pathways provided by the fossil record. The necessity of preservation of an effective occlusion has been hypothesized to functionally constrain crown morphological changes and to also facilitate convergent evolution. The Muroidea superfamily constitutes a relevant model for the study of molar crown diversification because it encompasses one third of the extant mammalian biodiversity. Methodology/Principal Findings Combined microwear and 3D-topographic analyses performed on fossil and extant muroid molars allow for a first quantification of the relationships between changes in crown morphology and functionality of occlusion. Based on an abundant fossil record and on a well resolved phylogeny, our results show that the most derived functional condition associates longitudinal chewing and non interlocking of cusps. This condition has been reached at least 7 times within muroids via two main types of evolutionary pathways each respecting functional continuity. In the first type, the flattening of tooth crown which induces the removal of cusp interlocking occurs before the rotation of the chewing movement. In the second type however, flattening is subsequent to rotation of the chewing movement which can be associated with certain changes in cusp morphology. Conclusion/Significance The reverse orders of the changes involved in these different pathways reveal a mosaic evolution of mammalian dentition in which direction of chewing and crown shape seem to be partly decoupled. Either can change in respect to strong functional constraints affecting occlusion which thereby limit the number of the possible pathways. Because convergent

  5. Mosaic convergence of rodent dentitions.

    Directory of Open Access Journals (Sweden)

    Vincent Lazzari

    Full Text Available BACKGROUND: Understanding mechanisms responsible for changes in tooth morphology in the course of evolution is an area of investigation common to both paleontology and developmental biology. Detailed analyses of molar tooth crown shape have shown frequent homoplasia in mammalian evolution, which requires accurate investigation of the evolutionary pathways provided by the fossil record. The necessity of preservation of an effective occlusion has been hypothesized to functionally constrain crown morphological changes and to also facilitate convergent evolution. The Muroidea superfamily constitutes a relevant model for the study of molar crown diversification because it encompasses one third of the extant mammalian biodiversity. METHODOLOGY/PRINCIPAL FINDINGS: Combined microwear and 3D-topographic analyses performed on fossil and extant muroid molars allow for a first quantification of the relationships between changes in crown morphology and functionality of occlusion. Based on an abundant fossil record and on a well resolved phylogeny, our results show that the most derived functional condition associates longitudinal chewing and non interlocking of cusps. This condition has been reached at least 7 times within muroids via two main types of evolutionary pathways each respecting functional continuity. In the first type, the flattening of tooth crown which induces the removal of cusp interlocking occurs before the rotation of the chewing movement. In the second type however, flattening is subsequent to rotation of the chewing movement which can be associated with certain changes in cusp morphology. CONCLUSION/SIGNIFICANCE: The reverse orders of the changes involved in these different pathways reveal a mosaic evolution of mammalian dentition in which direction of chewing and crown shape seem to be partly decoupled. Either can change in respect to strong functional constraints affecting occlusion which thereby limit the number of the possible

  6. Entropy and gravity concepts as new methodological indexes to investigate technological convergence: patent network-based approach.

    Directory of Open Access Journals (Sweden)

    Yongrae Cho

    Full Text Available The volatility and uncertainty in the process of technological developments are growing faster than ever due to rapid technological innovations. Such phenomena result in integration among disparate technology fields. At this point, it is a critical research issue to understand the different roles and the propensity of each element technology for technological convergence. In particular, the network-based approach provides a holistic view in terms of technological linkage structures. Furthermore, the development of new indicators based on network visualization can reveal the dynamic patterns among disparate technologies in the process of technological convergence and provide insights for future technological developments. This research attempts to analyze and discover the patterns of the international patent classification codes of the United States Patent and Trademark Office's patent data in printed electronics, which is a representative technology in the technological convergence process. To this end, we apply the physical idea as a new methodological approach to interpret technological convergence. More specifically, the concepts of entropy and gravity are applied to measure the activities among patent citations and the binding forces among heterogeneous technologies during technological convergence. By applying the entropy and gravity indexes, we could distinguish the characteristic role of each technology in printed electronics. At the technological convergence stage, each technology exhibits idiosyncratic dynamics which tend to decrease technological differences and heterogeneity. Furthermore, through nonlinear regression analysis, we have found the decreasing patterns of disparity over a given total period in the evolution of technological convergence. This research has discovered the specific role of each element technology field and has consequently identified the co-evolutionary patterns of technological convergence. These new findings

  7. Synthesis and Characterization of Organic Impurities in Bortezomib Anhydride Produced by a Convergent Technology

    Science.gov (United States)

    Ivanov, Andrey S.; Shishkov, Sergey V.; Zhalnina, Anna A.

    2012-01-01

    A profile of impurities in bortezomib anhydride, produced by a recently developed convergent technology, has been characterized. HPLC-MS analysis of the drug essence revealed three impurities: an epimer of bortezomib, resulting from partial racemization of l-phenylalanine’s stereogenic center during the chemical synthesis, and two epimeric products of oxidative degradation of bortezomib, in which boron is replaced by the OH group. The impurities were obtained by chemical synthesis and characterized by physical methods. PMID:22396904

  8. Genome-wide signatures of convergent evolution in echolocating mammals.

    Science.gov (United States)

    Parker, Joe; Tsagkogeorga, Georgia; Cotton, James A; Liu, Yuan; Provero, Paolo; Stupka, Elia; Rossiter, Stephen J

    2013-10-10

    Evolution is typically thought to proceed through divergence of genes, proteins and ultimately phenotypes. However, similar traits might also evolve convergently in unrelated taxa owing to similar selection pressures. Adaptive phenotypic convergence is widespread in nature, and recent results from several genes have suggested that this phenomenon is powerful enough to also drive recurrent evolution at the sequence level. Where homoplasious substitutions do occur these have long been considered the result of neutral processes. However, recent studies have demonstrated that adaptive convergent sequence evolution can be detected in vertebrates using statistical methods that model parallel evolution, although the extent to which sequence convergence between genera occurs across genomes is unknown. Here we analyse genomic sequence data in mammals that have independently evolved echolocation and show that convergence is not a rare process restricted to several loci but is instead widespread, continuously distributed and commonly driven by natural selection acting on a small number of sites per locus. Systematic analyses of convergent sequence evolution in 805,053 amino acids within 2,326 orthologous coding gene sequences compared across 22 mammals (including four newly sequenced bat genomes) revealed signatures consistent with convergence in nearly 200 loci. Strong and significant support for convergence among bats and the bottlenose dolphin was seen in numerous genes linked to hearing or deafness, consistent with an involvement in echolocation. Unexpectedly, we also found convergence in many genes linked to vision: the convergent signal of many sensory genes was robustly correlated with the strength of natural selection. This first attempt to detect genome-wide convergent sequence evolution across divergent taxa reveals the phenomenon to be much more pervasive than previously recognized.

  9. Convergence Time Evaluation of Algorithms in MANETs

    CERN Document Server

    Patil, Annapurna P; Chunhaviriyakul, Krittaya

    2009-01-01

    Since the advent of wireless communication, the need for mobile ad hoc networks has been growing exponentially. This has opened up a Pandoras Box of algorithms for dealing with mobile ad hoc networks, or MANETs, as they are generally referred to. Most attempts made at evaluating these algorithms so far have focused on parameters such as throughput, packet delivery ratio, overhead etc. An analysis of the convergence times of these algorithms is still an open issue. The work carried out fills this gap by evaluating the algorithms on the basis of convergence time. Algorithms for MANETs can be classified into three categories: reactive, proactive, and hybrid protocols. In this project, we compare the convergence times of representative algorithms in each category, namely Ad hoc On Demand Distance Vector (AODV) reactive, Destination Sequence Distance Vector protocol (DSDV) proactive, and Temporally Ordered Routing Algorithm (TORA) hybrid. The algorithm performances are compared by simulating them in ns2. Tcl is us...

  10. Convergence analysis of an upwind finite volume element method with Crouzeix-Raviart element for non-selfadjoint and indefinite problems

    Institute of Scientific and Technical Information of China (English)

    Hongxing RUI; Chunjia BI

    2008-01-01

    In this paper we construct an upwind finite volume element scheme based on the Crouzeix-Raviart nonconforming element for nonselfadjoint elliptic problems. These problems often appear in dealing with flow in porous media. We establish the optimal order H1-norm error estimate. We also give the uniform convergence under minimal elliptic regularity assumption.

  11. Converging divergences? An international comparison of the impact of globalization on industrial relations and employment careers : A critical analysis of Kwame Bediako's theology from a Frisian perspective.

    NARCIS (Netherlands)

    Mills, Melinda; Blossfeld, Hans-Peter; Buchholz, Sandra; Hofaecker, Dirk; Bernardi, Fabrizio; Hofmeister, Heather; Wagenaar, Hinne; Hofäcker, D.

    2008-01-01

    Profound social and economic transformations have taken place over the last two decades in modern societies. These changes are often referred to as globalization. The aim of this article is to examine whether processes of globalization have produced increasing convergence of employment-related aspec

  12. Metagenomic analysis of a permafrost microbial community reveals a rapid response to thaw

    Science.gov (United States)

    MacKelprang, R.; Waldrop, M.P.; Deangelis, K.M.; David, M.M.; Chavarria, K.L.; Blazewicz, S.J.; Rubin, E.M.; Jansson, J.K.

    2011-01-01

    Permafrost contains an estimated 1672????????Pg carbon (C), an amount roughly equivalent to the total currently contained within land plants and the atmosphere. This reservoir of C is vulnerable to decomposition as rising global temperatures cause the permafrost to thaw. During thaw, trapped organic matter may become more accessible for microbial degradation and result in greenhouse gas emissions. Despite recent advances in the use of molecular tools to study permafrost microbial communities, their response to thaw remains unclear. Here we use deep metagenomic sequencing to determine the impact of thaw on microbial phylogenetic and functional genes, and relate these data to measurements of methane emissions. Metagenomics, the direct sequencing of DNA from the environment, allows the examination of whole biochemical pathways and associated processes, as opposed to individual pieces of the metabolic puzzle. Our metagenome analyses reveal that during transition from a frozen to a thawed state there are rapid shifts in many microbial, phylogenetic and functional gene abundances and pathways. After one week of incubation at 5 ??C, permafrost metagenomes converge to be more similar to each other than while they are frozen. We find that multiple genes involved in cycling of C and nitrogen shift rapidly during thaw. We also construct the first draft genome from a complex soil metagenome, which corresponds to a novel methanogen. Methane previously accumulated in permafrost is released during thaw and subsequently consumed by methanotrophic bacteria. Together these data point towards the importance of rapid cycling of methane and nitrogen in thawing permafrost. ?? 2011 Macmillan Publishers Limited. All rights reserved.

  13. Analysis of self-overlap reveals trade-offs in plankton swimming trajectories

    DEFF Research Database (Denmark)

    Mariani, Patrizio; Visser, Andre W.; Mazzocchi, Maria Grazia

    2014-01-01

    these contrasting processes. This trade-off can be hypothesized as being evident in the behaviour of plankton, which inhabit a dilute three-dimensional environment with few refuges or orienting landmarks. We present an analysis of the swimming path geometries based on a volumetric Monte Carlo sampling approach......, which is particularly adept at revealing such trade-offs by measuring the self-overlap of the trajectories. Application of this method to experimentally measured trajectories reveals that swimming patterns in copepods are shaped to efficiently explore volumes at small scales, while achieving a large...

  14. Proteomic and genomic analysis reveals novel Campylobacter jejuni outer membrane proteins and potential heterogeneity.

    Science.gov (United States)

    Watson, Eleanor; Sherry, Aileen; Inglis, Neil F; Lainson, Alex; Jyothi, Dushyanth; Yaga, Raja; Manson, Erin; Imrie, Lisa; Everest, Paul; Smith, David G E

    2014-09-01

    Gram-negative bacterial outer membrane proteins play important roles in the interaction of bacteria with their environment including nutrient acquisition, adhesion and invasion, and antibiotic resistance. In this study we identified 47 proteins within the Sarkosyl-insoluble fraction of Campylobacter jejuni 81-176, using LC-ESI-MS/MS. Comparative analysis of outer membrane protein sequences was visualised to reveal protein distribution within a panel of Campylobacter spp., identifying several C. jejuni-specific proteins. Smith-Waterman analyses of C. jejuni homologues revealed high sequence conservation amongst a number of hypothetical proteins, sequence heterogeneity of other proteins and several proteins which are absent in a proportion of strains.

  15. Institutional and Socio-Economic Convergence in the European Union

    Directory of Open Access Journals (Sweden)

    Jordi López-Tamayo

    2014-12-01

    Full Text Available The objective of this paper is to analyze convergence in institutional, social, and macroeconomic conditions between EU member states. Our analysis covers the period 1995-2013 and considers the potential impact of the Great Recession. With this aim, we use a composite indicator that combines information from 51 hard and soft indicators, and we estimate convergence equations for the composite indicator and its seven dimensions considering different country groups. The obtained results show evidence of conditional convergence among EU member states but limited evidence of unconditional convergence over the considered period.

  16. Specialisation and Convergence in European Regions

    Directory of Open Access Journals (Sweden)

    Enrico Marelli

    2007-09-01

    Full Text Available The purpose of this paper was to analyze specialization and convergence of European countries and regions, within the framework of integration in the EU. This is important not only for long-term real convergence processes, but also for a proper functioning of the monetary union (in the line of research on the OCA's criteria, asymmetry of shocks and synchronization of business cycles. The position of new member states is particularly delicate, also considering the forthcoming adoption of the euro by some of them. As indicated by the EU Treaty, economic growth should be balanced with economic and social cohesion that includes a careful consideration of regional disparities. Our empirical investigation focuses on the regions of EU25, further broken up into other relevant groupings (EU15, EMU, and the new members' EU10 group, over the period from 1980 (or 1990 for EU10 to 2005. This paper considers a rather fine regional disaggregation (NUTS-2 level, counting 250 regions. The analysis of different indices of specialisation point to a prevalent increase of homogeneity of sector structures across European regions, although in some cases (especially in the industrial sector and in some services specialisation has increased. For convergence, a sigma convergence's analysis confirms a reduction of disparities, both at a country and regional level. However, a trade-off between fast national growth and internal distribution has emerged in the early stages of development, as in the case of new members. Moreover, beta convergence has also been established - regarding per capita income, employment and productivity - for almost all territorial aggregates (excluding the new members since 1999. The addition of structural variables, following a beta-conditional approach, indicates a positive role for services and a negative impact of agriculture. Finally, some preliminary results have been obtained by the innovative inclusion of specialisation indices within

  17. Thalamic alterations in preterm neonates and their relation to ventral striatum disturbances revealed by a combined shape and pose analysis.

    Science.gov (United States)

    Lao, Yi; Wang, Yalin; Shi, Jie; Ceschin, Rafael; Nelson, Marvin D; Panigrahy, Ashok; Leporé, Natasha

    2016-01-01

    Finding the neuroanatomical correlates of prematurity is vital to understanding which structures are affected, and to designing efficient prevention and treatment strategies. Converging results reveal that thalamic abnormalities are important indicators of prematurity. However, little is known about the localization of the abnormalities within the subnuclei of the thalamus, or on the association of altered thalamic development with other deep gray matter disturbances. Here, we aim to investigate the effect of prematurity on the thalamus and the putamen in the neonatal brain, and further investigate the associated abnormalities between these two structures. Using brain structural magnetic resonance imaging, we perform a novel combined shape and pose analysis of the thalamus and putamen between 17 preterm (41.12 ± 5.08 weeks) and 19 term-born (45.51 ± 5.40 weeks) neonates at term equivalent age. We also perform a set of correlation analyses between the thalamus and the putamen, based on the surface and pose results. We locate significant alterations on specific surface regions such as the anterior and ventral anterior (VA) thalamic nuclei, and significant relative pose changes of the left thalamus and the right putamen. In addition, we detect significant association between the thalamus and the putamen for both surface and pose parameters. The regions that are significantly associated include the VA, and the anterior and inferior putamen. We detect statistically significant surface deformations and pose changes on the thalamus and putamen, and for the first time, demonstrate the feasibility of using relative pose parameters as indicators for prematurity in neonates. Our methods show that regional abnormalities of the thalamus are associated with alterations of the putamen, possibly due to disturbed development of shared pre-frontal connectivity. More specifically, the significantly correlated regions in these two structures point to frontal

  18. Song convergence in multiple urban populations of silvereyes (Zosterops lateralis).

    Science.gov (United States)

    Potvin, Dominique A; Parris, Kirsten M

    2012-08-01

    Recent studies have revealed differences between urban and rural vocalizations of numerous bird species. These differences include frequency shifts, amplitude shifts, altered song speed, and selective meme use. If particular memes sung by urban populations are adapted to the urban soundscape, "urban-typical" calls, memes, or repertoires should be consistently used in multiple urban populations of the same species, regardless of geographic location. We tested whether songs or contact calls of silvereyes (Zosterops lateralis) might be subject to such convergent cultural evolution by comparing syllable repertoires of geographically dispersed urban and rural population pairs throughout southeastern Australia. Despite frequency and tempo differences between urban and rural calls, call repertoires were similar between habitat types. However, certain song syllables were used more frequently by birds from urban than rural populations. Partial redundancy analysis revealed that both geographic location and habitat characteristics were important predictors of syllable repertoire composition. These findings suggest convergent cultural evolution: urban populations modify both song and call syllables from their local repertoire in response to noise.

  19. The relations of real and nominal convergence in the EU with impacts on the euro area participation

    OpenAIRE

    Magdaléna DRASTICHOVÁ

    2012-01-01

    Real convergence of economies in macroeconomic terms is accompanied by convergence of nominal variables. The paper provides an analysis of the relationship of the GDP per capita development as an indicator of the real convergence and the development of the comparable price level as a representative of the nominal convergence in relation to the EU average value in the EU economies. Using the concept of convergence, the development of variability of real and nominal convergence indi...

  20. CONVERGENCE RATE OF A GENERALIZED ADDITIVE SCHWARZ ALGORITHM

    Institute of Scientific and Technical Information of China (English)

    Jin-ping Zeng; Gao-jie Chen

    2006-01-01

    The convergence rate of a generalized additive Schwarz algorithm for solving boundary value problems of elliptic partial differential equations is studied. A quantitative analysis of the convergence rate is given for the model Dirichlet problem. It will be shown that a greater acceleration of the algorithm can be obtained by choosing the parameter suitably.Some numerical tests are also presented in this paper.

  1. Dynamic transcriptional signature and cell fate analysis reveals plasticity of individual neural plate border cells.

    Science.gov (United States)

    Roellig, Daniela; Tan-Cabugao, Johanna; Esaian, Sevan; Bronner, Marianne E

    2017-03-29

    The 'neural plate border' of vertebrate embryos contains precursors of neural crest and placode cells, both defining vertebrate characteristics. How these lineages segregate from neural and epidermal fates has been a matter of debate. We address this by performing a fine-scale quantitative temporal analysis of transcription factor expression in the neural plate border of chick embryos. The results reveal significant overlap of transcription factors characteristic of multiple lineages in individual border cells from gastrula through neurula stages. Cell fate analysis using a Sox2 (neural) enhancer reveals that cells that are initially Sox2+ cells can contribute not only to neural tube but also to neural crest and epidermis. Moreover, modulating levels of Sox2 or Pax7 alters the apportionment of neural tube versus neural crest fates. Our results resolve a long-standing question and suggest that many individual border cells maintain ability to contribute to multiple ectodermal lineages until or beyond neural tube closure.

  2. Adaptation to different types of stress converge on mitochondrial metabolism

    DEFF Research Database (Denmark)

    Lahtvee, Petri-Jaan; Kumar, Rahul; Hallstrom, B. M.

    2016-01-01

    Yeast cell factories encounter physical and chemical stresses when used for industrial production of fuels and chemicals. These stresses reduce productivity and increase bioprocess costs. Understanding the mechanisms of the stress response is essential for improving cellular robustness in platform...... strains. We investigated the three most commonly encountered industrial stresses for yeast (ethanol, salt, and temperature) to identify the mechanisms of general and stress-specific responses under chemostat conditions in which specific growth rate–dependent changes are eliminated. By applying systems......-level analysis, we found that most stress responses converge on mitochondrial processes. Our analysis revealed that stress-specific factors differ between applied stresses; however, they are underpinned by an increased ATP demand. We found that when ATP demand increases to high levels, respiration cannot provide...

  3. Proteomics Analysis of Ovarian Cancer Cell Lines and Tissues Reveals Drug Resistance-associated Proteins

    Science.gov (United States)

    CRUZ*, ISA N.; COLEY*, HELEN M.; KRAMER, HOLGER B.; MADHURI, THUMULURU KAVITAH; SAFUWAN, NUR A.M.; ANGELINO, ANA RITA; YANG, MIN

    2016-01-01

    Background: Carboplatin and paclitaxel form the cornerstone of chemotherapy for epithelial ovarian cancer, however, drug resistance to these agents continues to present challenges. Despite extensive research, the mechanisms underlying this resistance remain unclear. Materials and Methods: A 2D-gel proteomics method was used to analyze protein expression levels of three human ovarian cancer cell lines and five biopsy samples. Representative proteins identified were validated via western immunoblotting. Ingenuity pathway analysis revealed metabolomic pathway changes. Results: A total of 189 proteins were identified with restricted criteria. Combined treatment targeting the proteasome-ubiquitin pathway resulted in re-sensitisation of drug-resistant cells. In addition, examination of five surgical biopsies of ovarian tissues revealed α-enolase (ENOA), elongation factor Tu, mitochondrial (EFTU), glyceraldehyde-3-phosphate dehydrogenase (G3P), stress-70 protein, mitochondrial (GRP75), apolipoprotein A-1 (APOA1), peroxiredoxin (PRDX2) and annexin A (ANXA) as candidate biomarkers of drug-resistant disease. Conclusion: Proteomics combined with pathway analysis provided information for an effective combined treatment approach overcoming drug resistance. Analysis of cell lines and tissues revealed potential prognostic biomarkers for ovarian cancer. *These Authors contributed equally to this study. PMID:28031236

  4. IMS-based Analysis and Research of Fixed Mobile Convergence%基于IMS固移网络融合的分析与研究

    Institute of Scientific and Technical Information of China (English)

    冯艳华

    2011-01-01

    本文介绍了IMS定义和及特性,进一步阐述了IMS网络架构,深入研究了基于IMS的固移融合网络的演进趋势和技术方案,阐述了IMS对固移网络融合的作用,为运营商制定网络演进提出了方案和建议。%This paper describes the IMS and the definition and characteristics,further elaborated the IMS network architecture,in-depth study of the IMS-based fixed mobile convergence trends and the evolution of network technology program,describes the IMS network convergence on the role of solid shift for operators to develop network evolution plans and proposals put forward.

  5. 我国媒介融合的内外动因解析%Analysis of the Internal and External Causes of Media Convergence in China

    Institute of Scientific and Technical Information of China (English)

    郑晓华

    2011-01-01

    Convergence is a prominent feature in the process of media development at present. In order to acquaint with the phenomenon of media convergence and master the disciplines of media convergence, we should explore the media convergence motivations in two di%融合是当前媒介发展进程中的一个显著特征。为更好地认识媒介融合的现象,把握媒介融合的规律,从科技发展的历史和媒介发展的现实两个维度考察我国媒介融合现象,可以看到,媒介生态环境及媒介形态环境革命性、指向性变化是促进媒介融合的内在动因,媒介产业的发展是媒介趋向融合的外部力量。现实语境中,探析媒介融合的内外动因,有利于了解新闻传播方式、媒介内容生产方式的变化过程,推进媒介进一步融合,促进传媒业的整体发展;同时也能给当前媒体从业人员提供重要的启示,为我国新闻业界和学界变革提供重要的现实依据。

  6. ANALYSIS OF THE IMPACT OF THE CONVERGENCE PROCESS TO THE INTERNATIONAL ACCOUNTING STANDARDS IN BRAZIL: A STUDY BASED ON THE VALUE RELEVANCE OF ACCOUNTING INFORMATION

    OpenAIRE

    2014-01-01

    Recently, a lot of changes in the legislation governing the Brazilian accounting practices initiated the alignment of Brazil to the internationalization process of accounting. In this context, this article aims to verify if the process of convergence to international accounting standards impacted the value relevance of accounting information such as Earnings per Share (LPA) and Equity per Share (PLPA), of the non-financial companies most traded on BM&FBOVESPA. This is done by testing the ...

  7. 山东省农业产业结构同构的实证分析%Empirical Analysis of Convergence of Agriculture Structure in Shandong Province

    Institute of Scientific and Technical Information of China (English)

    吴珂

    2015-01-01

    针对我国农产品“滞销、卖难、买贵”、农产品过剩等现象,试图从产业结构趋同方面进行研究解释。应用产业结构相似系数法和Krugman结构差异度指数法从三次产业层面和农业层面对山东省的产业结构趋同现象进行研究,发现三次产业层面产业结构同构现象明显,但在农业内部有所减弱。根据分析结果提出了相关对策。%In view of the features of “dull of sale, difficult to sell, high in purchasing, supply over demand” of our agricultural product, this study attempts to explain from the industrial structure convergence. Coefficient method and Krugman structure difference degree index method are used to study the convergence of industrial structure in Shandong Province from the three level of industry and Agriculture. It finds that three industry level industrial structure convergence phenomenon is obvious, but in a reduced level of Agriculture. And lastly this study puts forward corresponding countermeasures.

  8. Balkanized Research in Ecological Engineering Revealed by a Bibliometric Analysis of Earthworms and Ecosystem Services

    Science.gov (United States)

    Blouin, Manuel; Sery, Nicolas; Cluzeau, Daniel; Brun, Jean-Jacques; Bédécarrats, Alain

    2013-08-01

    Energy crisis, climate changes, and biodiversity losses have reinforced the drive for more ecologically-based approaches for environmental management. Such approaches are characterized by the use of organisms rather than energy-consuming technologies. Although earthworms are believed to be potentially useful organisms for managing ecosystem services, there is actually no quantification of such a trend in literature. This bibliometric analysis aimed to measure the evolution of the association of "earthworms" and other terms such as ecosystem services (primary production, nutrient cycling, carbon sequestration, soil structure, and pollution remediation), "ecological engineering" or "biodiversity," to assess their convergence or divergence through time. In this aim, we calculated the similarity index, an indicator of the paradigmatic proximity defined in applied epistemology, for each year between 1900 and 2009. We documented the scientific fields and the geographical origins of the studies, as well as the land uses, and compare these characteristics with a 25 years old review on earthworm management. The association of earthworm related keywords with ecosystem services related keywords was increasing with time, reflecting the growing interest in earthworm use in biodiversity and ecosystem services management. Conversely, no significant increase in the association between earthworms and disciplines such as ecological engineering or restoration ecology was observed. This demonstrated that general ecologically-based approaches have yet to emerge and that there is little exchange of knowledge, methods or concepts among balkanized application realms. Nevertheless, there is a strong need for crossing the frontiers between fields of application and for developing an umbrella discipline to provide a framework for the use of organisms to manage ecosystem services.

  9. Balkanized research in ecological engineering revealed by a bibliometric analysis of earthworms and ecosystem services.

    Science.gov (United States)

    Blouin, Manuel; Sery, Nicolas; Cluzeau, Daniel; Brun, Jean-Jacques; Bédécarrats, Alain

    2013-08-01

    Energy crisis, climate changes, and biodiversity losses have reinforced the drive for more ecologically-based approaches for environmental management. Such approaches are characterized by the use of organisms rather than energy-consuming technologies. Although earthworms are believed to be potentially useful organisms for managing ecosystem services, there is actually no quantification of such a trend in literature. This bibliometric analysis aimed to measure the evolution of the association of "earthworms" and other terms such as ecosystem services (primary production, nutrient cycling, carbon sequestration, soil structure, and pollution remediation), "ecological engineering" or "biodiversity," to assess their convergence or divergence through time. In this aim, we calculated the similarity index, an indicator of the paradigmatic proximity defined in applied epistemology, for each year between 1900 and 2009. We documented the scientific fields and the geographical origins of the studies, as well as the land uses, and compare these characteristics with a 25 years old review on earthworm management. The association of earthworm related keywords with ecosystem services related keywords was increasing with time, reflecting the growing interest in earthworm use in biodiversity and ecosystem services management. Conversely, no significant increase in the association between earthworms and disciplines such as ecological engineering or restoration ecology was observed. This demonstrated that general ecologically-based approaches have yet to emerge and that there is little exchange of knowledge, methods or concepts among balkanized application realms. Nevertheless, there is a strong need for crossing the frontiers between fields of application and for developing an umbrella discipline to provide a framework for the use of organisms to manage ecosystem services.

  10. Notes on Some Convergences in Riesz Space

    Institute of Scientific and Technical Information of China (English)

    CHEN Zhi-jie; AI Fu-ju

    2007-01-01

    An equivalent description of u-uniform convergence is presented first. Then the relations among the order convergence,u-uniform convergence and norm convergence of sequences are discussed in Riesz spaces. An equivalence of the three convergences is brought forward; namely, {fn} is a u-uniform Cauchy sequence. Finally the relations among the three convergences of sequences am also extended to the relations among the convergences of nets in Riesz spaces.

  11. New molecular phenotypes in the dst mutants of Arabidopsis revealed by DNA microarray analysis.

    Science.gov (United States)

    Pérez-Amador, M A; Lidder, P; Johnson, M A; Landgraf, J; Wisman, E; Green, P J

    2001-12-01

    In this study, DNA microarray analysis was used to expand our understanding of the dst1 mutant of Arabidopsis. The dst (downstream) mutants were isolated originally as specifically increasing the steady state level and the half-life of DST-containing transcripts. As such, txhey offer a unique opportunity to study rapid sequence-specific mRNA decay pathways in eukaryotes. These mutants show a threefold to fourfold increase in mRNA abundance for two transgenes and an endogenous gene, all containing DST elements, when examined by RNA gel blot analysis; however, they show no visible aberrant phenotype. Here, we use DNA microarrays to identify genes with altered expression levels in dst1 compared with the parental plants. In addition to verifying the increase in the transgene mRNA levels, which were used to isolate these mutants, we were able to identify new genes with altered mRNA abundance in dst1. RNA gel blot analysis confirmed the microarray data for all genes tested and also was used to catalog the first molecular differences in gene expression between the dst1 and dst2 mutants. These differences revealed previously unknown molecular phenotypes for the dst mutants that will be helpful in future analyses. Cluster analysis of genes altered in dst1 revealed new coexpression patterns that prompt new hypotheses regarding the nature of the dst1 mutation and a possible role of the DST-mediated mRNA decay pathway in plants.

  12. Econometric Analysis on Regional Economic Disparity and Sigma Convergence -- A Case of Liaoning Province%区域经济差异的σ收敛空间计量分析——以辽宁省为例

    Institute of Scientific and Technical Information of China (English)

    许燕

    2011-01-01

    An initial study is made on alternation of regional economic disparity by calculating variation coefficient, Gini coefficient and Theil index based on the real per capita GDP across 14 cities from 1995 to 2006 in Liaoning, and constructs spatial econometric model to study convergences in Liaoning Province. According to the analysis, it is found out that regional economic growth in Liaoning Province takes on staggered convergences within the whole time regional. On this basis, spatial econometric model is built to study the the σ convergence of regional economic disparities of Liaoning Province.%根据1995--2008年辽宁省各市实际人均GDP数据,通过计算变异系数、基尼系数和塞尔指标对辽宁省区域经济差距的变动状况进行初步研究。研究发现,在整个时间区域上,辽宁省区域经济差距呈阶段性。在此基础上,构建经济空间计量模型对辽宁省区域经济差异进行σ收敛研究。

  13. CONVERGENCE OF TECHNOLOGIES

    Directory of Open Access Journals (Sweden)

    R. Siriram

    2012-01-01

    Full Text Available

    ENGLISH ABSTRACT: Technology is a catalyst for competitive advantage. However, it is how technology is used that leads to a firm’s improved performance. In this article, an investigative framework is constructed to understand better what strategically drives new technology adoption. The strategic drivers include technology and business strategy alignment, better technology planning and selection of new technologies, the effects on a firm’s culture and climate, links to a firm’s organisational and environmental evolution, and benefits through convergence and collaboration. Using an investigative framework, it is shown how the strategic drivers link to improve a firm’s performance, producing competitive advantage. The investigative framework is tested using structural equation modelling. Various hypotheses are formed, and recommendations for further research are made.

    AFRIKAANSE OPSOMMING: Tegnologie is ‘n katalisator vir mededingende voordeel. Dit is egter hoe tegnologie aangewend word wat aanleiding gee tot ‘n onderneming se verbeterde prestasie. In hierdie artikel word ‘n ondersoekende raamwerk gekonstrueer om insig te kry in dit wat die aanvaarding van nuwe tegnologie strategies dryf. Die strategiese dryfvere sluit in die belyning van tegnologie en ondernemingstrategie, beter tegnologiebeplanning en seleksie van nuwe tegnologieë, die effek op ‘n onderneming se kultuur en klimaat, koppeling na ‘n onderneming se organisatoriese en omgewingsevolusie, en voordele verkry deur konvergensie en samewerking. Deur ‘n ondersoekende raamwerk te gebruik, word daar getoon dat die strategiese dryfvere koppel om ‘n onderneming se prestasie te verbeter en sodoende ‘n mededingende voordeel te skep. Die raamwerk word getoets en hipoteses geformuleer waarna aanbevelings oor verdere navorsing aan die hand gedoen word.

  14. The Convergence of Intelligences

    Science.gov (United States)

    Diederich, Joachim

    Minsky (1985) argued an extraterrestrial intelligence may be similar to ours despite very different origins. ``Problem- solving'' offers evolutionary advantages and individuals who are part of a technical civilisation should have this capacity. On earth, the principles of problem-solving are the same for humans, some primates and machines based on Artificial Intelligence (AI) techniques. Intelligent systems use ``goals'' and ``sub-goals'' for problem-solving, with memories and representations of ``objects'' and ``sub-objects'' as well as knowledge of relations such as ``cause'' or ``difference.'' Some of these objects are generic and cannot easily be divided into parts. We must, therefore, assume that these objects and relations are universal, and a general property of intelligence. Minsky's arguments from 1985 are extended here. The last decade has seen the development of a general learning theory (``computational learning theory'' (CLT) or ``statistical learning theory'') which equally applies to humans, animals and machines. It is argued that basic learning laws will also apply to an evolved alien intelligence, and this includes limitations of what can be learned efficiently. An example from CLT is that the general learning problem for neural networks is intractable, i.e. it cannot be solved efficiently for all instances (it is ``NP-complete''). It is the objective of this paper to show that evolved intelligences will be constrained by general learning laws and will use task-decomposition for problem-solving. Since learning and problem-solving are core features of intelligence, it can be said that intelligences converge despite very different origins.

  15. Local coexistence of VO2 phases revealed by deep data analysis

    Science.gov (United States)

    Strelcov, Evgheni; Ievlev, Anton; Belianinov, Alex; Tselev, Alexander; Kolmakov, Andrei; Kalinin, Sergei V.

    2016-07-01

    We report a synergistic approach of micro-Raman spectroscopic mapping and deep data analysis to study the distribution of crystallographic phases and ferroelastic domains in a defected Al-doped VO2 microcrystal. Bayesian linear unmixing revealed an uneven distribution of the T phase, which is stabilized by the surface defects and uneven local doping that went undetectable by other classical analysis techniques such as PCA and SIMPLISMA. This work demonstrates the impact of information recovery via statistical analysis and full mapping in spectroscopic studies of vanadium dioxide systems, which is commonly substituted by averaging or single point-probing approaches, both of which suffer from information misinterpretation due to low resolving power.

  16. Network analysis of oyster transcriptome revealed a cascade of cellular responses during recovery after heat shock.

    Directory of Open Access Journals (Sweden)

    Lingling Zhang

    Full Text Available Oysters, as a major group of marine bivalves, can tolerate a wide range of natural and anthropogenic stressors including heat stress. Recent studies have shown that oysters pretreated with heat shock can result in induced heat tolerance. A systematic study of cellular recovery from heat shock may provide insights into the mechanism of acquired thermal tolerance. In this study, we performed the first network analysis of oyster transcriptome by reanalyzing microarray data from a previous study. Network analysis revealed a cascade of cellular responses during oyster recovery after heat shock and identified responsive gene modules and key genes. Our study demonstrates the power of network analysis in a non-model organism with poor gene annotations, which can lead to new discoveries that go beyond the focus on individual genes.

  17. Molecular mechanisms involved in convergent crop domestication.

    Science.gov (United States)

    Lenser, Teresa; Theißen, Günter

    2013-12-01

    Domestication has helped to understand evolution. We argue that, vice versa, novel insights into evolutionary principles could provide deeper insights into domestication. Molecular analyses have demonstrated that convergent phenotypic evolution is often based on molecular changes in orthologous genes or pathways. Recent studies have revealed that during plant domestication the causal mutations for convergent changes in key traits are likely to be located in particular genes. These insights may contribute to defining candidate genes for genetic improvement during the domestication of new plant species. Such efforts may help to increase the range of arable crops available, thus increasing crop biodiversity and food security to help meet the predicted demands of the continually growing global population under rapidly changing environmental conditions.

  18. Neural networks convergence using physicochemical data.

    Science.gov (United States)

    Karelson, Mati; Dobchev, Dimitar A; Kulshyn, Oleksandr V; Katritzky, Alan R

    2006-01-01

    An investigation of the neural network convergence and prediction based on three optimization algorithms, namely, Levenberg-Marquardt, conjugate gradient, and delta rule, is described. Several simulated neural networks built using the above three algorithms indicated that the Levenberg-Marquardt optimizer implemented as a back-propagation neural network converged faster than the other two algorithms and provides in most of the cases better prediction. These conclusions are based on eight physicochemical data sets, each with a significant number of compounds comparable to that usually used in the QSAR/QSPR modeling. The superiority of the Levenberg-Marquardt algorithm is revealed in terms of functional dependence of the change of the neural network weights with respect to the gradient of the error propagation as well as distribution of the weight values. The prediction of the models is assessed by the error of the validation sets not used in the training process.

  19. Proteomic and genomic analysis reveals novel Campylobacter jejuni outer membrane proteins and potential heterogeneity

    Directory of Open Access Journals (Sweden)

    Eleanor Watson

    2014-09-01

    Full Text Available Gram-negative bacterial outer membrane proteins play important roles in the interaction of bacteria with their environment including nutrient acquisition, adhesion and invasion, and antibiotic resistance. In this study we identified 47 proteins within the Sarkosyl-insoluble fraction of Campylobacter jejuni 81-176, using LC–ESI-MS/MS. Comparative analysis of outer membrane protein sequences was visualised to reveal protein distribution within a panel of Campylobacter spp., identifying several C. jejuni-specific proteins. Smith–Waterman analyses of C. jejuni homologues revealed high sequence conservation amongst a number of hypothetical proteins, sequence heterogeneity of other proteins and several proteins which are absent in a proportion of strains.

  20. Proteome-wide analysis of arginine monomethylation reveals widespread occurrence in human cells

    DEFF Research Database (Denmark)

    Larsen, Sara C; Sylvestersen, Kathrine B; Mund, Andreas

    2016-01-01

    as coactivator-associated arginine methyltransferase 1 (CARM1)] or PRMT1 increased the RNA binding function of HNRNPUL1. High-content single-cell imaging additionally revealed that knocking down CARM1 promoted the nuclear accumulation of SRSF2, independent of cell cycle phase. Collectively, the presented human...... kidney 293 cells, indicating that the occurrence of this modification is comparable to phosphorylation and ubiquitylation. A site-level conservation analysis revealed that arginine methylation sites are less evolutionarily conserved compared to arginines that were not identified as modified...... to the frequency of somatic mutations at arginine methylation sites throughout the proteome, we observed that somatic mutations were common at arginine methylation sites in proteins involved in mRNA splicing. Furthermore, in HeLa and U2OS cells, we found that distinct arginine methyltransferases differentially...

  1. Transcriptome-based network analysis reveals a spectrum model of human macrophage activation.

    Science.gov (United States)

    Xue, Jia; Schmidt, Susanne V; Sander, Jil; Draffehn, Astrid; Krebs, Wolfgang; Quester, Inga; De Nardo, Dominic; Gohel, Trupti D; Emde, Martina; Schmidleithner, Lisa; Ganesan, Hariharasudan; Nino-Castro, Andrea; Mallmann, Michael R; Labzin, Larisa; Theis, Heidi; Kraut, Michael; Beyer, Marc; Latz, Eicke; Freeman, Tom C; Ulas, Thomas; Schultze, Joachim L

    2014-02-20

    Macrophage activation is associated with profound transcriptional reprogramming. Although much progress has been made in the understanding of macrophage activation, polarization, and function, the transcriptional programs regulating these processes remain poorly characterized. We stimulated human macrophages with diverse activation signals, acquiring a data set of 299 macrophage transcriptomes. Analysis of this data set revealed a spectrum of macrophage activation states extending the current M1 versus M2-polarization model. Network analyses identified central transcriptional regulators associated with all macrophage activation complemented by regulators related to stimulus-specific programs. Applying these transcriptional programs to human alveolar macrophages from smokers and patients with chronic obstructive pulmonary disease (COPD) revealed an unexpected loss of inflammatory signatures in COPD patients. Finally, by integrating murine data from the ImmGen project we propose a refined, activation-independent core signature for human and murine macrophages. This resource serves as a framework for future research into regulation of macrophage activation in health and disease.

  2. Electrically active defects in silica-filled epoxy as revealed by light emission analysis

    Energy Technology Data Exchange (ETDEWEB)

    Aubert, E; Teyssedre, G; Laurent, C [Universite de Toulouse, UPS, INPT, LAPLACE (Laboratoire Plasma et Conversion d' Energie), 118 route de Narbonne, F-31062 Toulouse Cedex 9 (France); Rowe, S; Robiani, S, E-mail: christian.laurent@laplace.univ-tlse.f [Schneider Electric, Direction des Recherches Materiaux, rue Henri Tarze, F-38050 Grenoble (France)

    2009-08-21

    Epoxy resins have long been used as the insulation of electrical systems. They are generally formulated with a dispersion of micro-fillers to improve thermal and mechanical properties. However, there are concerns about the possible influence of these fillers on the electric behaviour, especially on the long term ageing under functional stresses. At the loose interface between matrix and fillers, macro- and micro-voids in the resin can provide weak points that are difficult to detect using conventional spectroscopy. Light emission analysis from the material under electrical stress is an efficient way to reveal such electrically active defects since internal ionizing events would give rise to photon emission. A detailed analysis of the light emitted by silica-filled and unfilled epoxy samples is presented. The photon counting technique, spectral analysis and imaging give a firm basis to discuss the contributing emission processes to the detected signal. They reveal the existence of ionizing events into internal defects. The sensitivity of the optical method is order of magnitudes higher than the sensitivity of conventional partial discharge detection.

  3. Phylogenomic Analysis Reveals Extensive Phylogenetic Mosaicism in the Human GPCR Superfamily

    Directory of Open Access Journals (Sweden)

    Mathew Woodwark

    2007-01-01

    Full Text Available A novel high throughput phylogenomic analysis (HTP was applied to the rhodopsin G-protein coupled receptor (GPCR family. Instances of phylogenetic mosaicism between receptors were found to be frequent, often as instances of correlated mosaicism and repeated mosaicism. A null data set was constructed with the same phylogenetic topology as the rhodopsin GPCRs. Comparison of the two data sets revealed that mosaicism was found in GPCRs in a higher frequency than would be expected by homoplasy or the effects of topology alone. Various evolutionary models of differential conservation, recombination and homoplasy are explored which could result in the patterns observed in this analysis. We find that the results are most consistent with frequent recombination events. A complex evolutionary history is illustrated in which it is likely frequent recombination has endowed GPCRs with new functions. The pattern of mosaicism is shown to be informative for functional prediction for orphan receptors. HTP analysis is complementary to conventional phylogenomic analyses revealing mosaicism that would not otherwise have been detectable through conventional phylogenetics.

  4. Multilocus sequence analysis of nectar pseudomonads reveals high genetic diversity and contrasting recombination patterns.

    Directory of Open Access Journals (Sweden)

    Sergio Alvarez-Pérez

    Full Text Available The genetic and evolutionary relationships among floral nectar-dwelling Pseudomonas 'sensu stricto' isolates associated to South African and Mediterranean plants were investigated by multilocus sequence analysis (MLSA of four core housekeeping genes (rrs, gyrB, rpoB and rpoD. A total of 35 different sequence types were found for the 38 nectar bacterial isolates characterised. Phylogenetic analyses resulted in the identification of three main clades [nectar groups (NGs 1, 2 and 3] of nectar pseudomonads, which were closely related to five intrageneric groups: Pseudomonas oryzihabitans (NG 1; P. fluorescens, P. lutea and P. syringae (NG 2; and P. rhizosphaerae (NG 3. Linkage disequilibrium analysis pointed to a mostly clonal population structure, even when the analysis was restricted to isolates from the same floristic region or belonging to the same NG. Nevertheless, signatures of recombination were observed for NG 3, which exclusively included isolates retrieved from the floral nectar of insect-pollinated Mediterranean plants. In contrast, the other two NGs comprised both South African and Mediterranean isolates. Analyses relating diversification to floristic region and pollinator type revealed that there has been more unique evolution of the nectar pseudomonads within the Mediterranean region than would be expected by chance. This is the first work analysing the sequence of multiple loci to reveal geno- and ecotypes of nectar bacteria.

  5. The role of the internet in the cultural convergence: a theoretical analysis based on the dynamic-constructivist approach from the consumers’ online interactions perspective

    Directory of Open Access Journals (Sweden)

    IGNACIO TAMAYO TORRES

    2008-06-01

    Full Text Available This paper poses, from a theoretical perspective, the role that may have Internet as a key factor to foster the cultural convergence among countries. In this regard, we refl ect about a central theoretic proposal, taking as main reference the dynamic constructivist approach. The gist of the paper goes down the lines of how shared values generated in the on-line communication processes developed by individuals (consumers from different cultures-either between each other or between them and the medium-, can be passed on to every culture of origin, fostering their rapprochement in the long run.

  6. Existence and convergence to a propagating terrace in one-dimensional reaction-diffusion equations

    CERN Document Server

    Ducrot, Arnaud; Matano, Hiroshi

    2012-01-01

    We consider one-dimensional reaction-diffusion equations for a large class of spatially periodic nonlinearities (including multistable ones) and study the asymptotic behavior of solutions with Heaviside type initial data. Our analysis reveals some new dynamics where the profile of the propagation is not characterized by a single front, but by a layer of several fronts which we call a terrace. Existence and convergence to such a terrace is proven by using an intersection number argument, without much relying on standard linear analysis. Hence, on top of the peculiar phenomenon of propagation that our work highlights, several corollaries will follow on the existence and convergence to pulsating traveling fronts even for highly degenerate nonlinearities that have not been treated before.

  7. VNTR analysis reveals unexpected genetic diversity within Mycoplasma agalactiae, the main causative agent of contagious agalactia

    Directory of Open Access Journals (Sweden)

    Ayling Roger D

    2008-11-01

    Full Text Available Abstract Background Mycoplasma agalactiae is the main cause of contagious agalactia, a serious disease of sheep and goats, which has major clinical and economic impacts. Previous studies of M. agalactiae have shown it to be unusually homogeneous and there are currently no available epidemiological techniques which enable a high degree of strain differentiation. Results We have developed variable number tandem repeat (VNTR analysis using the sequenced genome of the M. agalactiae type strain PG2. The PG2 genome was found to be replete with tandem repeat sequences and 4 were chosen for further analysis. VNTR 5 was located within the hypothetical protein MAG6170 a predicted lipoprotein. VNTR 14 was intergenic between the hypothetical protein MAG3350 and the hypothetical protein MAG3340. VNTR 17 was intergenic between the hypothetical protein MAG4060 and the hypothetical protein MAG4070 and VNTR 19 spanned the 5' end of the pseudogene for a lipoprotein MAG4310 and the 3' end of the hypothetical lipoprotein MAG4320. We have investigated the genetic diversity of 88 M. agalactiae isolates of wide geographic origin using VNTR analysis and compared it with pulsed field gel electrophoresis (PFGE and random amplified polymorphic DNA (RAPD analysis. Simpson's index of diversity was calculated to be 0.324 for PFGE and 0.574 for VNTR analysis. VNTR analysis revealed unexpected diversity within M. agalactiae with 9 different VNTR types discovered. Some correlation was found between geographical origin and the VNTR type of the isolates. Conclusion VNTR analysis represents a useful, rapid first-line test for use in molecular epidemiological analysis of M. agalactiae for outbreak tracing and control.

  8. Sustained fixation induced changes in phoria and convergence peak velocity.

    Directory of Open Access Journals (Sweden)

    Eun H Kim

    Full Text Available PURPOSE: This study sought to investigate the influence of phoria adaptation on convergence peak velocity from responses located at different initial vergence positions. METHODS: Symmetrical 4° convergence step responses and near dissociated phoria (measured at 40 cm from the subject's midline were recorded from six subjects with normal binocular vision using an infrared limbus tracking system with a haploscope. Two different sustained fixations (1° and 16° convergent rotation along the subject's midline were used to study whether phoria had an influence on the peak velocity of convergence responses located at two initial vergence positions (1° or 'far' steps and 12° or 'near' steps. RESULTS: Phoria was significantly adapted after a sustained fixation task at near (16° and far (1° (p<0.002. A repeated measures ANOVA showed that convergence far steps were significantly faster than the near steps (p<0.03. When comparing convergence steps with the same initial vergence position, steps measured after near phoria adaptation were faster than responses after far adaptation (p<0.02. A regression analysis demonstrated that the change in phoria and the change in convergence peak velocity were significantly correlated for the far convergence steps (r = 0.97, p = 0.001. A weaker correlation was observed for the near convergence steps (r = 0.59, p = 0.20. CONCLUSION: As a result of sustained fixation, phoria was adapted and the peak velocity of the near and far convergence steps was modified. This study has clinical considerations since prisms, which evoke phoria adaptation, can be prescribed to help alleviate visual discomfort. Future investigations should include a systematic study of how prisms may influence convergence and divergence eye movements for those prescribed with prisms within their spectacles.

  9. OPTIMAL CONVERGENCE RATE OF THE LANDAU EQUATION WITH FRICTIONAL FORCE

    Institute of Scientific and Technical Information of China (English)

    Liu Shuangqian; Liu Hongxia

    2012-01-01

    The Cauchy problem of the Landau equation with frictional force is investigated.Based on Fourier analysis and nonlinear energy estimates,the optimal convergence rate to the steady state is obtained under some conditions on initial data.

  10. On the Convergence of Broyden-Like Methods

    Institute of Scientific and Technical Information of China (English)

    Ioannis K. ARGYROS

    2007-01-01

    The author provides a finer local as well as semilocal convergence analysis of a certain class of Broyden-like methods for solving equations containing a nondifferentiable term on the m-dimensional Euclidean space (m≥1 a natural number).

  11. Chromosome-specific segmentation revealed by structural analysis of individually isolated chromosomes.

    Science.gov (United States)

    Kitada, Kunio; Taima, Akira; Ogasawara, Kiyomoto; Metsugi, Shouichi; Aikawa, Satoko

    2011-04-01

    Analysis of structural rearrangements at the individual chromosomal level is still technologically challenging. Here we optimized a chromosome isolation method using fluorescent marker-assisted laser-capture and laser-beam microdissection and applied it to structural analysis of two aberrant chromosomes found in a lung cancer cell line. A high-density array-comparative genomic hybridization (array-CGH) analysis of DNA samples prepared from each of the chromosomes revealed that these two chromosomes contained 296 and 263 segments, respectively, ranging from 1.5 kb to 784.3 kb in size, derived from different portions of chromosome 8. Among these segments, 242 were common in both aberrant chromosomes, but 75 were found to be chromosome-specific. Sequences of 263 junction sites connecting the ends of segments were determined using a PCR/Sanger-sequencing procedure. Overlapping microhomologies were found at 169 junction sites. Junction partners came from various portions of chromosome 8 and no biased pattern in the positional distribution of junction partners was detected. These structural characteristics suggested the occurrence of random fragmentation of the entire chromosome 8 followed by random rejoining of these fragments. Based on that, we proposed a model to explain how these aberrant chromosomes are formed. Through these structural analyses, it was demonstrated that the optimized chromosome isolation method described here can provide high-quality chromosomal DNA for high resolution array-CGH analysis and probably for massively parallel sequencing analysis.

  12. Global Positioning System Technology (GPS for Psychological Research: A Test of Convergent and Nomological Validity

    Directory of Open Access Journals (Sweden)

    Pedro eWolf

    2013-06-01

    Full Text Available The purpose of this paper is to examine the convergent and nomological validity of a GPS-based measure of daily activity, operationalized as Number of Places Visited (NPV. Relations among the GPS-based measure and two self-report measures of NPV, as well as relations among NPV and two factors made up of self-reported individual differences were examined. The first factor was composed of variables related to an Active Lifestyle (AL (e.g. positive affect, extraversion… and the second factor was composed of variables related to a Sedentary Lifestyle (SL (e.g. depression, neuroticism…. NPV was measured over a four-day period. This timeframe was made up of two week and two weekend days. A bi-variate analysis established one level of convergent validity and a Split-Plot GLM examined convergent validity, nomological validity, and alternative hypotheses related to constraints on activity throughout the week simultaneously. The first analysis revealed significant correlations among NPV measures- weekday, weekend, and the entire four day blocks, supporting the convergent validity of the Diary-, Google Maps-, and GPS-NPV measures. Results from the second analysis, indicating non-significant mean differences in NPV regardless of method, also support this conclusion. We also found that AL is a statistically significant predictor of NPV no matter how NPV was measured. We did not find a statically significant relation among NPV and SL. These results permit us to infer that the GPS-based NPV measure has convergent and nomological validity.

  13. CONVERGENCE ANALYSIS IN SENSE OF LEBESGUE-p NORM OF DECENTRALIZED NON-REPETITIVE ITERATIVE LEARNING CONTROL FOR LINEAR LARGE-SCALE SYSTEMS

    Institute of Scientific and Technical Information of China (English)

    Xiaoe RUAN; Huizhuo WU; Na LI; Baiwu WAN

    2009-01-01

    In this paper, a decentralized iterative learning control strategy is embedded into the procedure of hierarchical steady-state optimization for a class of linear large-scale industrial processes which consists of a number of subsystems. The task of the learning controller for each subsystem is to iteratively generate a sequence of upgraded control inputs to take responsibilities of a sequential step functional control signals with distinct scales which are determined by the local decision-making units in the two-layer hierarchical steady-state optimization processing. The objective of the designated strategy is to consecutively improve the transient performance of the system. By means of the generalized Young inequality of convolution integral, the convergence of the learning algorithm is analyzed in the sense of Lebesgue-p norm. It is shown that the inherent feature of system such as the multi-dimensionality and the interaction may influence the convergence of the non-repetitive learning rule. Numerical simulations illustrate the effectiveness of the proposed control scheme and the validity of the conclusion.

  14. Convergence Analysis of a FV-FE Scheme for Partially Miscible Two-Phase Flow in Anisotropic Porous Media

    KAUST Repository

    Saad, Bilal Mohammed

    2014-01-01

    We study the convergence of a combined finite volume nonconforming finite element scheme on general meshes for a partially miscible two-phase flow model in anisotropic porous media. This model includes capillary effects and exchange between the phase. The diffusion term,which can be anisotropic and heterogeneous, is discretized by piecewise linear nonconforming triangular finite elements. The other terms are discretized by means of a cell-centered finite volume scheme on a dual mesh. The relative permeability of each phase is decentred according the sign of the velocity at the dual interface. The convergence of the scheme is proved thanks to an estimate on the two pressures which allows to show estimates on the discrete time and compactness results in the case of degenerate relative permeabilities. A key point in the scheme is to use particular averaging formula for the dissolution function arising in the diffusion term. We show also a simulation of CO2 injection in a water saturated reservoir and nuclear waste management. Numerical results are obtained by in-house numerical code. © Springer International Publishing Switzerland 2014.

  15. Proteomic analysis reveals novel proteins associated with progression and differentiation of colorectal carcinoma

    Directory of Open Access Journals (Sweden)

    Yi Gan

    2014-01-01

    Full Text Available Aim: The objective of this study is to characterize differential proteomic expression among well-differentiation and poor-differentiation colorectal carcinoma tissues and normal mucous epithelium. Materials and Methods: The study is based on quantitative 2-dimensional gel electrophoresis and analyzed by PDquest. Results: Excluding redundancies due to proteolysis and posttranslational modified isoforms of over 600 protein spots, 11 proteins were revealed as regulated with statistical variance being within the 95 th confidence level and were identified by peptide mass fingerprinting in matrix assisted laser desorption/ionization time-of-flight mass spectrometry. Progression-associated proteins belong to the functional complexes of tumorigenesis, proliferation, differentiation, metabolism, and the regulation of major histocompatibility complex processing and other functions. Partial but significant overlap was revealed with previous proteomics and transcriptomics studies in CRC. Among various differentiation stage of CRC tissues, we identified calreticulin precursor, MHC class I antigen (human leukocyte antigen A , glutathione S-transferase pi1, keratin 8, heat shock protein 27, tubulin beta chain, triosephosphate, fatty acid-binding protein, hemoglobin (deoxy mutant with val b 1 replaced by met (HBB, and zinc finger protein 312 (FEZF2. Conclusions: Their functional networks were analyzed by Ingenuity systems Ingenuity Pathways Analysis and revealed the potential roles as novel biomarkers for progression in various differentiation stages of CRC.

  16. Bifurcation Analysis of an Existing Mathematical Model Reveals Novel Treatment Strategies and Suggests Potential Cure for Type 1 Diabetes

    DEFF Research Database (Denmark)

    Nielsen, Kenneth Hagde Mandrup; Ottesen, Johnny T.; Pociot, Flemming

    2014-01-01

    Type 1 diabetes is a disease with serious personal and socioeconomic consequences that has attracted the attention of modellers recently. But as models of this disease tend to be complicated, there has been only limited mathematical analysis to date. Here we address this problem by providing...... a bifurcation analysis of a previously published mathematical model for the early stages of type 1 diabetes in diabetes-prone NOD mice, which is based on the data available in the literature. We also show positivity and the existence of a family of attracting trapping regions in the positive 5D cone, converging...... towards a smaller trapping region, which is the intersection over the family. All these trapping regions are compact sets, and thus, practical weak persistence is guaranteed. We conclude our analysis by proposing 4 novel treatment strategies: increasing the phagocytic ability of resting macrophages...

  17. Analysis of miRNA market trends reveals hotspots of research activity.

    Science.gov (United States)

    Oosta, Gary; Razvi, Enal

    2012-04-01

    We have conducted an analysis of the miRNA research marketplace by evaluating the publication trends in the field. In this article, we present the results of our analysis which reveals that hotspots exist in terms of research activities in the miRNA space--these hotspots illustrate the areas in the miRNA research space where specific miRNAs have been extensively studied, and other areas that represent new territory. We frame these data into the context of areas of opportunity for miRNA content harvest versus segments of opportunity for the development of research tools. Also presented in this article are the primary market data from online surveys we have performed with researchers involved in miRNA research around the world. Taken together, these data frame the current state of the miRNA marketplace and provide niches of opportunity for new entrants into this space.

  18. Global analysis of gene expression in pulmonary fibrosis reveals distinct programs regulating lung inflammation and fibrosis

    Science.gov (United States)

    Kaminski, Naftali; Allard, John D.; Pittet, Jean F.; Zuo, Fengrong; Griffiths, Mark J. D.; Morris, David; Huang, Xiaozhu; Sheppard, Dean; Heller, Renu A.

    2000-02-01

    The molecular mechanisms of pulmonary fibrosis are poorly understood. We have used oligonucleotide arrays to analyze the gene expression programs that underlie pulmonary fibrosis in response to bleomycin, a drug that causes lung inflammation and fibrosis, in two strains of susceptible mice (129 and C57BL/6). We then compared the gene expression patterns in these mice with 129 mice carrying a null mutation in the epithelial-restricted integrin 6 subunit (6/-), which develop inflammation but are protected from pulmonary fibrosis. Cluster analysis identified two distinct groups of genes involved in the inflammatory and fibrotic responses. Analysis of gene expression at multiple time points after bleomycin administration revealed sequential induction of subsets of genes that characterize each response. The availability of this comprehensive data set should accelerate the development of more effective strategies for intervention at the various stages in the development of fibrotic diseases of the lungs and other organs.

  19. Spaces of Ideal Convergent Sequences

    Directory of Open Access Journals (Sweden)

    M. Mursaleen

    2014-01-01

    Full Text Available In the present paper, we introduce some sequence spaces using ideal convergence and Musielak-Orlicz function ℳ=Mk. We also examine some topological properties of the resulting sequence spaces.

  20. Dynamical Convergence Trajectory in Networks

    Institute of Scientific and Technical Information of China (English)

    TAN Ning; ZHANG Yun-Jun; OUYANG Qi; GENG Zhi

    2005-01-01

    @@ It is well known that topology and dynamics are two major aspects to determine the function of a network. We study one of the dynamic properties of a network: trajectory convergence, i.e. how a system converges to its steady state. Using numerical and analytical methods, we show that in a logical-like dynamical model, the occurrence of convergent trajectory in a network depends mainly on the type of the fixed point and the ratio between activation and inhibition links. We analytically proof that this property is induced by the competition between two types of state transition structures in phase space: tree-like transition structure and star-like transition structure. We show that the biological networks, such as the cell cycle network in budding yeast, prefers the tree-like transition structures and suggest that this type of convergence trajectories may be universal.

  1. Analysis of economic convergence within Chiapas municipalities, regions and apparent inconsistencies Análisis de convergencia económica en el interior de Chiapas: municipios, regiones e inconsistencias aparentes

    Directory of Open Access Journals (Sweden)

    Óscar PELÁEZ HERREROS

    2012-06-01

    Full Text Available The data of annual per capita income estimated by the United Nations Development Programme (UNDP for the municipal human development index in Mexico shows that, in Chiapas, during the period 2000-2005, there was a strong convergence at the municipal level while there was no regional convergence. To explain this apparent inconsistency, the article «Regression towards mediocrity in hereditary stature», published by Galton in 1886, is examined, concluding that the clustering level of the data (municipal or regional determines the convergence analysis results, because the grouping of municipalities in regions causes loss of information that can generate phenomena of «regression towards the mean» or just the opposite, as is the case. In Chiapas, the municipal convergence would have led to convergence within the regions but not between regions.A partir de los datos de ingreso per cápita anual estimados por el Programa de las Naciones Unidas para el Desarrollo (PNUD en la elaboración del índice de desarrollo humano municipal en México, se comprueba que, en el interior de Chiapas durante el periodo 2000- 2005, se dio un proceso de fuerte convergencia a nivel municipal al tiempo que no hubo convergencia regional. Para explicar esta aparente inconsistencia, se reexamina el artículo «Regression towards mediocrity in hereditary stature» publicado por Galton en 1886, concluyendo que el nivel de agrupación de los datos (municipal o regional condiciona los resultados del análisis de convergencia, ya que la agrupación de los municipios en regiones origina pérdidas de información que pueden generar fenómenos de «regresión hacia la media» o justo lo contrario, como es el caso. En Chiapas, la convergencia municipal habría dado lugar a la convergencia dentro de las regiones, pero no entre regiones.

  2. Convergent and divergent mechanisms of sugar recognition across kingdoms.

    Science.gov (United States)

    Taylor, Maureen E; Drickamer, Kurt

    2014-10-01

    Protein modules that bind specific oligosaccharides are found across all kingdoms of life from single-celled organisms to man. Different, overlapping and evolving designations for sugar-binding domains in proteins can sometimes obscure common features that often reflect convergent solutions to the problem of distinguishing sugars with closely similar structures and binding them with sufficient affinity to achieve biologically meaningful results. Structural and functional analysis has revealed striking parallels between protein domains with widely different structures and evolutionary histories that employ common solutions to the sugar recognition problem. Recent studies also demonstrate that domains descended from common ancestors through divergent evolution appear more widely across the kingdoms of life than had previously been recognized.

  3. Weak entropy inequalities and entropic convergence

    Institute of Scientific and Technical Information of China (English)

    GAO FuQing; LI LiNa

    2008-01-01

    A criterion for algebraic convergence of the entropy is presented and an algebraic convergence result for the entropy of an exclusion process is improved.A weak entropy inequality is considered and its relationship to entropic convergence is discussed.

  4. Weak entropy inequalities and entropic convergence

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    A criterion for algebraic convergence of the entropy is presented and an algebraic convergence result for the entropy of an exclusion process is improved. A weak entropy inequality is considered and its relationship to entropic convergence is discussed.

  5. Convergence of knowledge, technology and society beyond convergence of nano-bio-info-cognitive technologies

    CERN Document Server

    Bainbridge, William; Tonn, Bruce; Whitesides, George

    2013-01-01

    Convergence of knowledge and technology for the benefit of society (CKTS) is the core opportunity for progress in the 21st century, based on five principles: (1) the interdependence of all components of nature and society, (2) enhancement of creativity and innovation through evolutionary processes of convergence that combine existing principles, and divergence that generates new ones, (3) decision analysis for research and development based on system-logic deduction, (4) higher-level cross-domain languages to generate new solutions and support transfer of new knowledge, and (5) vision-inspired basic research embodied in grand challenges. Solutions are outlined for key societal challenges, including creating new industries and jobs, improving lifelong wellness and human potential, achieving personalized and integrated healthcare and education, and securing a sustainable quality of life for all. This report provides a ten-year “NBIC2” vision within a longer-term framework for converging technology and human...

  6. Dynamic transcriptional signature and cell fate analysis reveals plasticity of individual neural plate border cells

    Science.gov (United States)

    Roellig, Daniela; Tan-Cabugao, Johanna; Esaian, Sevan; Bronner, Marianne E

    2017-01-01

    The ‘neural plate border’ of vertebrate embryos contains precursors of neural crest and placode cells, both defining vertebrate characteristics. How these lineages segregate from neural and epidermal fates has been a matter of debate. We address this by performing a fine-scale quantitative temporal analysis of transcription factor expression in the neural plate border of chick embryos. The results reveal significant overlap of transcription factors characteristic of multiple lineages in individual border cells from gastrula through neurula stages. Cell fate analysis using a Sox2 (neural) enhancer reveals that cells that are initially Sox2+ cells can contribute not only to neural tube but also to neural crest and epidermis. Moreover, modulating levels of Sox2 or Pax7 alters the apportionment of neural tube versus neural crest fates. Our results resolve a long-standing question and suggest that many individual border cells maintain ability to contribute to multiple ectodermal lineages until or beyond neural tube closure. DOI: http://dx.doi.org/10.7554/eLife.21620.001 PMID:28355135

  7. A beamformer analysis of MEG data reveals frontal generators of the musically elicited mismatch negativity.

    Directory of Open Access Journals (Sweden)

    Claudia Lappe

    Full Text Available To localize the neural generators of the musically elicited mismatch negativity with high temporal resolution we conducted a beamformer analysis (Synthetic Aperture Magnetometry, SAM on magnetoencephalography (MEG data from a previous musical mismatch study. The stimuli consisted of a six-tone melodic sequence comprising broken chords in C- and G-major. The musical sequence was presented within an oddball paradigm in which the last tone was lowered occasionally (20% by a minor third. The beamforming analysis revealed significant right hemispheric neural activation in the superior temporal (STC, inferior frontal (IFC, superior frontal (SFC and orbitofrontal (OFC cortices within a time window of 100-200 ms after the occurrence of a deviant tone. IFC and SFC activation was also observed in the left hemisphere. The pronounced early right inferior frontal activation of the auditory mismatch negativity has not been shown in MEG studies so far. The activation in STC and IFC is consistent with earlier electroencephalography (EEG, optical imaging and functional magnetic resonance imaging (fMRI studies that reveal the auditory and inferior frontal cortices as main generators of the auditory MMN. The observed right hemispheric IFC is also in line with some previous music studies showing similar activation patterns after harmonic syntactic violations. The results demonstrate that a deviant tone within a musical sequence recruits immediately a distributed neural network in frontal and prefrontal areas suggesting that top-down processes are involved when expectation violation occurs within well-known stimuli.

  8. Phenotypic Analysis Reveals that the 2010 Haiti Cholera Epidemic Is Linked to a Hypervirulent Strain.

    Science.gov (United States)

    Satchell, Karla J F; Jones, Christopher J; Wong, Jennifer; Queen, Jessica; Agarwal, Shivani; Yildiz, Fitnat H

    2016-09-01

    Vibrio cholerae O1 El Tor strains have been responsible for pandemic cholera since 1961. These strains have evolved over time, spreading globally in three separate waves. Wave 3 is caused by altered El Tor (AET) variant strains, which include the strain with the signature ctxB7 allele that was introduced in 2010 into Haiti, where it caused a devastating epidemic. In this study, we used phenotypic analysis to compare an early isolate from the Haiti epidemic to wave 1 El Tor isolates commonly used for research. It is demonstrated that the Haiti isolate has increased production of cholera toxin (CT) and hemolysin, increased motility, and a reduced ability to form biofilms. This strain also outcompetes common wave 1 El Tor isolates for colonization of infant mice, indicating that it has increased virulence. Monitoring of CT production and motility in additional wave 3 isolates revealed that this phenotypic variation likely evolved over time rather than in a single genetic event. Analysis of available whole-genome sequences and phylogenetic analyses suggested that increased virulence arose from positive selection for mutations found in known and putative regulatory genes, including hns and vieA, diguanylate cyclase genes, and genes belonging to the lysR and gntR regulatory families. Overall, the studies presented here revealed that V. cholerae virulence potential can evolve and that the currently prevalent wave 3 AET strains are both phenotypically distinct from and more virulent than many El Tor isolates.

  9. An International Comparative Analysis on China's Economic Growth and the Convergence in Energy Intensity Gap and Its Economic Mechanism%An International Comparative Analysis on China's Economic Growth and the Convergence in Energy Intensity Gap and Its Economic Mechanism

    Institute of Scientific and Technical Information of China (English)

    Qi Shaozhou; Yun Bo; Li Kai

    2011-01-01

    In this paper, the authors have empirically analyzed the convergence in per capita GDP gap and the convergence in the variation of energy intensity with respect to the change of per capita GDP between China and eight developed countries. Then, the authors run a regression on the impact of decisive factors of economic growth on energy intensity and its change, so as to find out the economic mechanism of energy intensity gap changing with respect to the variation of economic growth. This study concludes that: First, there is a convergence in per capita GDP gap between China and the eight developed countries. With the convergence in per capita GDP gap, the energy intensity gap between China and eight different countries also converge, and the convergence rate of the latter is faster than that of the former, i.e. if the per capita GDP gap between China and the eight developed countries decreases by 1%, the energy intensity gap between them will correspondingly decrease by 1.552%. Second, the energy intensity decreases with the improvement of industrial structure, the rising of energy prices, the advances of technology, and the expansion of investment in fixed assets, and it slightly increases with the increase of FDI. Third, the energy intensity gap between China and eight developed countries narrows with the lessening of the difference in fixed assets investment, energy prices, and technological progress between China and eight developed countries, yet increases with the narrowing of the difference in FDI, and has no significant correlation with the difference in industrial structure. Fourth, the narrowing of difference in per capita GDP between China and the eight developed countries can result in the lessening of energy intensity gap, whose economic mechanism is that the decisive factors, such as difference in investment, technology, and the competition mechanism of prices, which can determine the difference in economic growth, can significantly affect the energy

  10. Measure theory of statistical convergence

    Institute of Scientific and Technical Information of China (English)

    CHENG LiXin; LIN GuoChen; LAN YongYi; LIU Hui

    2008-01-01

    The question of establishing measure theory for statistical convergence has been moving closer to center stage, since a kind of reasonable theory is not only fundamental for unifying various kinds of statistical convergence, but also a bridge linking the studies of statistical convergence across measure theory, integration theory, probability and statistics. For this reason, this paper, in terms of subdifferential, first shows a representation theorem for all finitely additive probability measures defined on the σ-algebra of all subsets of N, and proves that every such measure can be uniquely decomposed into a convex combination of a countably additive probability measure and a statistical measure (i.e. a finitely additive probability measure μ with μ(k) = 0 for all singletons {k}). This paper also shows that classical statistical measures have many nice properties, such as: The set of all such measures endowed with the topology of point-wise convergence on forms a compact convex Hausdorff space; every classical statistical measure is of continuity type (hence, atomless), and every specific class of statistical measures fits a complementation minimax rule for every subset in N. Finally, this paper shows that every kind of statistical convergence can be unified in convergence of statistical measures.

  11. Measure theory of statistical convergence

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    The question of establishing measure theory for statistical convergence has been moving closer to center stage, since a kind of reasonable theory is not only fundamental for unifying various kinds of statistical convergence, but also a bridge linking the studies of statistical convergence across measure theory, integration theory, probability and statistics. For this reason, this paper, in terms of subdifferential, first shows a representation theorem for all finitely additive probability measures defined on the σ-algebra A of all subsets of N, and proves that every such measure can be uniquely decomposed into a convex combination of a countably additive probability measure and a statistical measure (i.e. a finitely additive probability measure μ with μ(k) = 0 for all singletons {k}). This paper also shows that classical statistical measures have many nice properties, such as: The set S of all such measures endowed with the topology of point-wise convergence on A forms a compact convex Hausdorff space; every classical statistical measure is of continuity type (hence, atomless), and every specific class of statistical measures fits a complementation minimax rule for every subset in N. Finally, this paper shows that every kind of statistical convergence can be unified in convergence of statistical measures.

  12. DOES THE MAASTRICHT CONVERGENCE CRITERIA WORK?

    Directory of Open Access Journals (Sweden)

    Karsai Zoltán-Krisztián

    2012-12-01

    Full Text Available During its 13 year history, the euro area experienced the most severe economic downturn in the late 2000s as a result of the 2007 financial-economic crisis stemming from the US banking sector. The crisis in the monetary union, besides posting a significant economic and social cost, revealed several weaknesses not just of the currency block as a whole, but also of its constituting members, which were masked by the prosperous economic environment characteristic for the 2000s. These conditions have put to the test the solidarity among the euro zone members, or in other words the existence of the currency block. One important problem of the currency block is the lack of harmony between the fiscal and economic policies of the member states, creating several and occasionally very divergent parts of the currency block. The aim of this research is to enhance the Maastricht convergence criteria’s and the Stability and Growth Pact’s role as a monitoring mechanism, allowing them to become more informative tools for the policy makers. For this, based on the relevant literature, we propose new potential explanatory variables which could enhance the role of the Maastricht convergence criteria and the Stability and Growth Pact. Some of the studied variables, like indebtedness of the private sector, capital flow compared to the size of the economy, government revenue compared to total public debt and current account balance help in enforcing the nominal convergence, while others (real labour productivity contribute to the real convergence. The explanatory power of the proposed variables are investigated in the case of France, Germany, Greece, Ireland, Italy, Portugal and Spain for the period comprised between 2000Q1-2011Q4. Results of the research show that with the exception of government revenue compared to total public debt, all proposed variables have significant explanatory power regarding the evolution of the state of the economy in all

  13. Gene set based integrated data analysis reveals phenotypic differences in a brain cancer model.

    Directory of Open Access Journals (Sweden)

    Kjell Petersen

    Full Text Available A key challenge in the data analysis of biological high-throughput experiments is to handle the often low number of samples in the experiments compared to the number of biomolecules that are simultaneously measured. Combining experimental data using independent technologies to illuminate the same biological trends, as well as complementing each other in a larger perspective, is one natural way to overcome this challenge. In this work we investigated if integrating proteomics and transcriptomics data from a brain cancer animal model using gene set based analysis methodology, could enhance the biological interpretation of the data relative to more traditional analysis of the two datasets individually. The brain cancer model used is based on serial passaging of transplanted human brain tumor material (glioblastoma--GBM through several generations in rats. These serial transplantations lead over time to genotypic and phenotypic changes in the tumors and represent a medically relevant model with a rare access to samples and where consequent analyses of individual datasets have revealed relatively few significant findings on their own. We found that the integrated analysis both performed better in terms of significance measure of its findings compared to individual analyses, as well as providing independent verification of the individual results. Thus a better context for overall biological interpretation of the data can be achieved.

  14. GENETIC DIVERSITY OF FIVE FRESHWATER MUSSELS IN GENUS ANODONTA (MOLLUSCA: BIVALVIA) REVEALED BY RAPD ANALYSIS

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    Unionidae(Bivalvia)are distributed infreshwaters,and represent a significanttaxonof benthic community[1].In China,freshwater mussels are abundant resources[2].Since1949,substantial investigations onthe unionidfau-na had been undertakenin China[3—8].Withreference tooverseas research[9,10],a preliminary reorganization onthe Unionidae was performed accordingtosome classifica-tion characteristics such as shell shape,larvae character-istics,and breeding habit[11].Due tothe serious conver-gence of freshwater muss...

  15. Diversity of eukaryotic DNA replication origins revealed by genome-wide analysis of chromatin structure.

    Directory of Open Access Journals (Sweden)

    Nicolas M Berbenetz

    2010-09-01

    Full Text Available Eukaryotic DNA replication origins differ both in their efficiency and in the characteristic time during S phase when they become active. The biological basis for these differences remains unknown, but they could be a consequence of chromatin structure. The availability of genome-wide maps of nucleosome positions has led to an explosion of information about how nucleosomes are assembled at transcription start sites, but no similar maps exist for DNA replication origins. Here we combine high-resolution genome-wide nucleosome maps with comprehensive annotations of DNA replication origins to identify patterns of nucleosome occupancy at eukaryotic replication origins. On average, replication origins contain a nucleosome depleted region centered next to the ACS element, flanked on both sides by arrays of well-positioned nucleosomes. Our analysis identified DNA sequence properties that correlate with nucleosome occupancy at replication origins genome-wide and that are correlated with the nucleosome-depleted region. Clustering analysis of all annotated replication origins revealed a surprising diversity of nucleosome occupancy patterns. We provide evidence that the origin recognition complex, which binds to the origin, acts as a barrier element to position and phase nucleosomes on both sides of the origin. Finally, analysis of chromatin reconstituted in vitro reveals that origins are inherently nucleosome depleted. Together our data provide a comprehensive, genome-wide view of chromatin structure at replication origins and suggest a model of nucleosome positioning at replication origins in which the underlying sequence occludes nucleosomes to permit binding of the origin recognition complex, which then (likely in concert with nucleosome modifiers and remodelers positions nucleosomes adjacent to the origin to promote replication origin function.

  16. Local Convergence Analysis of the Inexact Smoothing Levenberg-Marquardt Algorithm%非精确光滑化Levenberg-Marquardt算法的局部收敛性

    Institute of Scientific and Technical Information of China (English)

    崔蒙蒙; 凌晨

    2014-01-01

    非精确Levenberg-Marquardt(L-M)算法是求解非光滑约束方程组的重要算法之一。在将非光滑约束方程组等价转化成无约束方程的基础上,该文针对一种新的非精确光滑化L-M算法,在局部误差界条件下,得到此算法具有超线性或二次收敛性质。%The inexact Levenberg-Marquardt ( L-M) algorithm is one of the important algorithms for solving nonsmooth constraint equations .Based upon the unconstrained reformulation of the original problem , a new inexact smoothing L-M algorithm is presented .And under the local error bound condition , this algorithm enjoys the local superlinear or quadratic rate of convergence .

  17. Beyond division: convergences between postmodern qualitative research and family therapy.

    Science.gov (United States)

    De Haene, Lucia

    2010-01-01

    Starting from examples of postmodern research and therapeutic practice, we raise the question on the role of the research-therapy dichotomy within these approaches. The article aims to show the profound convergence between postmodern ethnographic research and constructionist, collaborative therapeutic approaches on a double, epistemological and practice level. First, we point out their converging development toward narrative and constructionist epistemologies. Second, an inquiry into the core features of these disciplinary activities' goal, process, and expert role reveals their profound convergence into a dialogical practice in which the boundaries between research and therapy are radically transgressed. We conclude by questioning the implications and acceptability of this convergence for researchers' and therapists' understanding of their practices.

  18. FDI, Human Capital and Income Convergence

    DEFF Research Database (Denmark)

    Völlmecke, Dominik; Jindra, Björn; Marek, Philipp

    2016-01-01

    This study examines income convergence in regional GDP per capita for a sample of 269 regions within the European Union (EU) between 2003 and 2010. We use an endogenous broad capital model based on foreign direct investment (FDI) induced agglomeration economies and human capital. By applying...... a Markov chain approach to a new dataset that exploits micro-aggregated sub-national FDI statistics, the analysis provides insights into regional income growth dynamics within the EU. Our results indicate a weak process of overall income convergence across EU regions. This does not apply to the dynamics...... within Central and East European countries (CEECs), where we find indications of a poverty trap. In contrast to FDI, regional human capital seems to be associated with higher income levels. However, we identify a positive interaction of FDI and human capital in their relation with income growth dynamics....

  19. Dynamic functional connectivity analysis reveals transient states of dysconnectivity in schizophrenia

    Directory of Open Access Journals (Sweden)

    E. Damaraju

    2014-01-01

    Full Text Available Schizophrenia is a psychotic disorder characterized by functional dysconnectivity or abnormal integration between distant brain regions. Recent functional imaging studies have implicated large-scale thalamo-cortical connectivity as being disrupted in patients. However, observed connectivity differences in schizophrenia have been inconsistent between studies, with reports of hyperconnectivity and hypoconnectivity between the same brain regions. Using resting state eyes-closed functional imaging and independent component analysis on a multi-site data that included 151 schizophrenia patients and 163 age- and gender matched healthy controls, we decomposed the functional brain data into 100 components and identified 47 as functionally relevant intrinsic connectivity networks. We subsequently evaluated group differences in functional network connectivity, both in a static sense, computed as the pairwise Pearson correlations between the full network time courses (5.4 minutes in length, and a dynamic sense, computed using sliding windows (44 s in length and k-means clustering to characterize five discrete functional connectivity states. Static connectivity analysis revealed that compared to healthy controls, patients show significantly stronger connectivity, i.e., hyperconnectivity, between the thalamus and sensory networks (auditory, motor and visual, as well as reduced connectivity (hypoconnectivity between sensory networks from all modalities. Dynamic analysis suggests that (1, on average, schizophrenia patients spend much less time than healthy controls in states typified by strong, large-scale connectivity, and (2, that abnormal connectivity patterns are more pronounced during these connectivity states. In particular, states exhibiting cortical–subcortical antagonism (anti-correlations and strong positive connectivity between sensory networks are those that show the group differences of thalamic hyperconnectivity and sensory hypoconnectivity

  20. Convergência local de renda no Brasil

    Directory of Open Access Journals (Sweden)

    Erika Cristina Barbosa de Almeida Ribeiro

    2012-09-01

    Full Text Available Diversos trabalhos verificam a hipótese de convergência de renda para as regiões brasileiras. Contudo, a persistência da desigualdade de renda ainda é verificada entre as regiões. Nesse contexto, utilizando a metodologia de Regressões Ponderadas Geograficamente (RPG, o presente trabalho busca analisar a hipótese de convergência local para as áreas mínimas comparáveis (AMCs. Os principais resultados confirmam a hipótese de múltiplos equilíbrios. Entretanto, de maneira mais sofisticada que a análise de clubes de convergência, os resultados da análise local indicam que cada AMC estaria convergindo para o seu específico estado estacionário.Several works verify the hypothesis of income convergence for Brazilian regions. However, income inequality's persistence can still be verified. In this context, using the methodology of Geographically Weighted Regression (GWR, this study aims to analyze the local income convergence among the Brazilians areas (AMCs. The main results indicate multiple equilibria hypotheses. But, in a more sophisticated manner than convergence clubs analysis, local analysis results indicate that each AMC would be converging to its specific steady state.

  1. Explaining slow convergence of EM in low noise linear mixtures

    OpenAIRE

    Petersen, Kaare Brandt; Winther, Ole

    2005-01-01

    This report conducts an investigation of the convergence properties of the EM algorithm used for linear mixture models. Since the linear mixture model is a rather general approach, the analysis is relevant for a wide range of models which to some degree are subsets of each other: Independent Component Analysis (ICA), probabilistic PCA, Factor Analysis (FA), Independent Factor Analysis (IFA) and Mean Field ICA.

  2. Rapid genome evolution in Pms1 region of rice revealed by comparative sequence analysis

    Institute of Scientific and Technical Information of China (English)

    YU JinSheng; FAN YouRong; LIU Nan; SHAN Yan; LI XiangHua; ZHANG QiFa

    2007-01-01

    Pms1, a locus for photoperiod sensitive genic male sterility in rice, was identified and mapped to chromosome 7 in previous studies. Here we report an effort to identify the candidate genes for Pms1 by comparative sequencing of BAC clones from two cultivars Minghui 63 and Nongken 58, the parents for the initial mapping population. Annotation and comparison of the sequences of the two clones resulted in a total of five potential candidates which should be functionally tested. We also conducted comparative analysis of sequences of these two cultivars with two other cultivars, Nipponbare and 93-11,for which sequence data were available in public databases. The analysis revealed large differences in sequence composition among the four genotypes in the Pms1 region primarily due to retroelement activity leading to rapid recent growth and divergence of the genomes. High levels of polymorphism in the forms of indels and SNPs were found both in intra- and inter-subspecific comparisons. Dating analysis using LTRs of the retroelements in this region showed that the substitution rate of LTRs was much higher than reported in the literature. The results provided strong evidence for rapid genomic evolution of this region as a consequence of natural and artificial selection.

  3. Potential microRNA-mediated oncogenic intercellular communication revealed by pan-cancer analysis

    Science.gov (United States)

    Li, Yue; Zhang, Zhaolei

    2014-11-01

    Carcinogenesis consists of oncogenesis and metastasis, and intriguingly microRNAs (miRNAs) are involved in both processes. Although aberrant miRNA activities are prevalent in diverse tumor types, the exact mechanisms for how they regulate cancerous processes are not always clear. To this end, we performed a large-scale pan-cancer analysis via a novel probabilistic approach to infer recurrent miRNA-target interactions implicated in 12 cancer types using data from The Cancer Genome Atlas. We discovered ~20,000 recurrent miRNA regulations, which are enriched for cancer-related miRNAs/genes. Notably, miRNA 200 family (miR-200/141/429) is among the most prominent miRNA regulators, which is known to be involved in metastasis. Importantly, the recurrent miRNA regulatory network is not only enriched for cancer pathways but also for extracellular matrix (ECM) organization and ECM-receptor interactions. The results suggest an intriguing cancer mechanism involving miRNA-mediated cell-to-cell communication, which possibly involves delivery of tumorigenic miRNA messengers to adjacent cells via exosomes. Finally, survival analysis revealed 414 recurrent-prognostic associations, where both gene and miRNA involved in each interaction conferred significant prognostic power in one or more cancer types. Together, our comprehensive pan-cancer analysis provided not only biological insights into metastasis but also brought to bear the clinical relevance of the proposed recurrent miRNA-gene associations.

  4. Integrated systems analysis reveals a molecular network underlying autism spectrum disorders.

    Science.gov (United States)

    Li, Jingjing; Shi, Minyi; Ma, Zhihai; Zhao, Shuchun; Euskirchen, Ghia; Ziskin, Jennifer; Urban, Alexander; Hallmayer, Joachim; Snyder, Michael

    2014-12-30

    Autism is a complex disease whose etiology remains elusive. We integrated previously and newly generated data and developed a systems framework involving the interactome, gene expression and genome sequencing to identify a protein interaction module with members strongly enriched for autism candidate genes. Sequencing of 25 patients confirmed the involvement of this module in autism, which was subsequently validated using an independent cohort of over 500 patients. Expression of this module was dichotomized with a ubiquitously expressed subcomponent and another subcomponent preferentially expressed in the corpus callosum, which was significantly affected by our identified mutations in the network center. RNA-sequencing of the corpus callosum from patients with autism exhibited extensive gene mis-expression in this module, and our immunochemical analysis showed that the human corpus callosum is predominantly populated by oligodendrocyte cells. Analysis of functional genomic data further revealed a significant involvement of this module in the development of oligodendrocyte cells in mouse brain. Our analysis delineates a natural network involved in autism, helps uncover novel candidate genes for this disease and improves our understanding of its molecular pathology.

  5. Comprehensive DNA Adduct Analysis Reveals Pulmonary Inflammatory Response Contributes to Genotoxic Action of Magnetite Nanoparticles

    Directory of Open Access Journals (Sweden)

    Kousuke Ishino

    2015-02-01

    Full Text Available Nanosized-magnetite (MGT is widely utilized in medicinal and industrial fields; however, its toxicological properties are not well documented. In our previous report, MGT showed genotoxicity in both in vitro and in vivo assay systems, and it was suggested that inflammatory responses exist behind the genotoxicity. To further clarify mechanisms underlying the genotoxicity, a comprehensive DNA adduct (DNA adductome analysis was conducted using DNA samples derived from the lungs of mice exposed to MGT. In total, 30 and 42 types of DNA adducts were detected in the vehicle control and MGT-treated groups, respectively. Principal component analysis (PCA against a subset of DNA adducts was applied and several adducts, which are deduced to be formed by inflammation or oxidative stress, as the case of etheno-deoxycytidine (εdC, revealed higher contributions to MGT exposure. By quantitative-LC-MS/MS analysis, εdC levels were significantly higher in MGT-treated mice than those of the vehicle control. Taken together with our previous data, it is suggested that inflammatory responses might be involved in the genotoxicity induced by MGT in the lungs of mice.

  6. Genomic and physiological analysis reveals versatile metabolic capacity of deep-sea Photobacterium phosphoreum ANT-2200.

    Science.gov (United States)

    Zhang, Sheng-Da; Santini, Claire-Lise; Zhang, Wei-Jia; Barbe, Valérie; Mangenot, Sophie; Guyomar, Charlotte; Garel, Marc; Chen, Hai-Tao; Li, Xue-Gong; Yin, Qun-Jian; Zhao, Yuan; Armengaud, Jean; Gaillard, Jean-Charles; Martini, Séverine; Pradel, Nathalie; Vidaud, Claude; Alberto, François; Médigue, Claudine; Tamburini, Christian; Wu, Long-Fei

    2016-05-01

    Bacteria of the genus Photobacterium thrive worldwide in oceans and show substantial eco-physiological diversity including free-living, symbiotic and piezophilic life styles. Genomic characteristics underlying this variability across species are poorly understood. Here we carried out genomic and physiological analysis of Photobacterium phosphoreum strain ANT-2200, the first deep-sea luminous bacterium of which the genome has been sequenced. Using optical mapping we updated the genomic data and reassembled it into two chromosomes and a large plasmid. Genomic analysis revealed a versatile energy metabolic potential and physiological analysis confirmed its growth capacity by deriving energy from fermentation of glucose or maltose, by respiration with formate as electron donor and trimethlyamine N-oxide (TMAO), nitrate or fumarate as electron acceptors, or by chemo-organo-heterotrophic growth in rich media. Despite that it was isolated at a site with saturated dissolved oxygen, the ANT-2200 strain possesses four gene clusters coding for typical anaerobic enzymes, the TMAO reductases. Elevated hydrostatic pressure enhances the TMAO reductase activity, mainly due to the increase of isoenzyme TorA1. The high copy number of the TMAO reductase isoenzymes and pressure-enhanced activity might imply a strategy developed by bacteria to adapt to deep-sea habitats where the instant TMAO availability may increase with depth.

  7. Topological robustness analysis of protein interaction networks reveals key targets for overcoming chemotherapy resistance in glioma

    Science.gov (United States)

    Azevedo, Hátylas; Moreira-Filho, Carlos Alberto

    2015-11-01

    Biological networks display high robustness against random failures but are vulnerable to targeted attacks on central nodes. Thus, network topology analysis represents a powerful tool for investigating network susceptibility against targeted node removal. Here, we built protein interaction networks associated with chemoresistance to temozolomide, an alkylating agent used in glioma therapy, and analyzed their modular structure and robustness against intentional attack. These networks showed functional modules related to DNA repair, immunity, apoptosis, cell stress, proliferation and migration. Subsequently, network vulnerability was assessed by means of centrality-based attacks based on the removal of node fractions in descending orders of degree, betweenness, or the product of degree and betweenness. This analysis revealed that removing nodes with high degree and high betweenness was more effective in altering networks’ robustness parameters, suggesting that their corresponding proteins may be particularly relevant to target temozolomide resistance. In silico data was used for validation and confirmed that central nodes are more relevant for altering proliferation rates in temozolomide-resistant glioma cell lines and for predicting survival in glioma patients. Altogether, these results demonstrate how the analysis of network vulnerability to topological attack facilitates target prioritization for overcoming cancer chemoresistance.

  8. Comparative Analysis of 35 Basidiomycete Genomes Reveals Diversity and Uniqueness of the Phylum

    Energy Technology Data Exchange (ETDEWEB)

    Riley, Robert; Salamov, Asaf; Otillar, Robert; Fagnan, Kirsten; Boussau, Bastien; Brown, Daren; Henrissat, Bernard; Levasseur, Anthony; Held, Benjamin; Nagy, Laszlo; Floudas, Dimitris; Morin, Emmanuelle; Manning, Gerard; Baker, Scott; Martin, Francis; Blanchette, Robert; Hibbett, David; Grigoriev, Igor V.

    2013-03-11

    Fungi of the phylum Basidiomycota (basidiomycetes), make up some 37percent of the described fungi, and are important in forestry, agriculture, medicine, and bioenergy. This diverse phylum includes symbionts, pathogens, and saprobes including wood decaying fungi. To better understand the diversity of this phylum we compared the genomes of 35 basidiomycete fungi including 6 newly sequenced genomes. The genomes of basidiomycetes span extremes of genome size, gene number, and repeat content. A phylogenetic tree of Basidiomycota was generated using the Phyldog software, which uses all available protein sequence data to simultaneously infer gene and species trees. Analysis of core genes reveals that some 48percent of basidiomycete proteins are unique to the phylum with nearly half of those (22percent) comprising proteins found in only one organism. Phylogenetic patterns of plant biomass-degrading genes suggest a continuum rather than a sharp dichotomy between the white rot and brown rot modes of wood decay among the members of Agaricomycotina subphylum. There is a correlation of the profile of certain gene families to nutritional mode in Agaricomycotina. Based on phylogenetically-informed PCA analysis of such profiles, we predict that that Botryobasidium botryosum and Jaapia argillacea have properties similar to white rot species, although neither has liginolytic class II fungal peroxidases. Furthermore, we find that both fungi exhibit wood decay with white rot-like characteristics in growth assays. Analysis of the rate of discovery of proteins with no or few homologs suggests the high value of continued sequencing of basidiomycete fungi.

  9. ANALYSIS OF THE CONVERGENCE RELATION BETWEEN D-N ALTERNATING PROCESS AND SCHWARTZ ALTERNATING PROCESS%D-N交替法与Schwartz交替法的收敛关系分析

    Institute of Scientific and Technical Information of China (English)

    孙连友; 洪伟

    2003-01-01

    This paper has demonstrated that the Schwartz alternating process must converge if D-N alternating process has converged. Its technique is that the overlapping regions in Schwartz alternating process are considered as independent domains, then Schwartz alternating process could be transformed into D-N alter-nating process. Finally the convergence estimation of Schwartz alternating process could be obtained. The results show that its convergence rate is same as that of D-N alternating process.

  10. A novel meta-analysis approach of cancer transcriptomes reveals prevailing transcriptional networks in cancer cells.

    Science.gov (United States)

    Niida, Atsushi; Imoto, Seiya; Nagasaki, Masao; Yamaguchi, Rui; Miyano, Satoru

    2010-01-01

    Although microarray technology has revealed transcriptomic diversities underlining various cancer phenotypes, transcriptional programs controlling them have not been well elucidated. To decode transcriptional programs governing cancer transcriptomes, we have recently developed a computational method termed EEM, which searches for expression modules from prescribed gene sets defined by prior biological knowledge like TF binding motifs. In this paper, we extend our EEM approach to predict cancer transcriptional networks. Starting from functional TF binding motifs and expression modules identified by EEM, we predict cancer transcriptional networks containing regulatory TFs, associated GO terms, and interactions between TF binding motifs. To systematically analyze transcriptional programs in broad types of cancer, we applied our EEM-based network prediction method to 122 microarray datasets collected from public databases. The data sets contain about 15000 experiments for tumor samples of various tissue origins including breast, colon, lung etc. This EEM based meta-analysis successfully revealed a prevailing cancer transcriptional network which functions in a large fraction of cancer transcriptomes; they include cell-cycle and immune related sub-networks. This study demonstrates broad applicability of EEM, and opens a way to comprehensive understanding of transcriptional networks in cancer cells.

  11. Dependency Network Analysis (DEPNA) Reveals Context Related Influence of Brain Network Nodes

    Science.gov (United States)

    Jacob, Yael; Winetraub, Yonatan; Raz, Gal; Ben-Simon, Eti; Okon-Singer, Hadas; Rosenberg-Katz, Keren; Hendler, Talma; Ben-Jacob, Eshel

    2016-01-01

    Communication between and within brain regions is essential for information processing within functional networks. The current methods to determine the influence of one region on another are either based on temporal resolution, or require a predefined model for the connectivity direction. However these requirements are not always achieved, especially in fMRI studies, which have poor temporal resolution. We thus propose a new graph theory approach that focuses on the correlation influence between selected brain regions, entitled Dependency Network Analysis (DEPNA). Partial correlations are used to quantify the level of influence of each node during task performance. As a proof of concept, we conducted the DEPNA on simulated datasets and on two empirical motor and working memory fMRI tasks. The simulations revealed that the DEPNA correctly captures the network’s hierarchy of influence. Applying DEPNA to the functional tasks reveals the dynamics between specific nodes as would be expected from prior knowledge. To conclude, we demonstrate that DEPNA can capture the most influencing nodes in the network, as they emerge during specific cognitive processes. This ability opens a new horizon for example in delineating critical nodes for specific clinical interventions. PMID:27271458

  12. Transcriptome analysis in tardigrade species reveals specific molecular pathways for stress adaptations.

    Science.gov (United States)

    Förster, Frank; Beisser, Daniela; Grohme, Markus A; Liang, Chunguang; Mali, Brahim; Siegl, Alexander Matthias; Engelmann, Julia C; Shkumatov, Alexander V; Schokraie, Elham; Müller, Tobias; Schnölzer, Martina; Schill, Ralph O; Frohme, Marcus; Dandekar, Thomas

    2012-01-01

    Tardigrades have unique stress-adaptations that allow them to survive extremes of cold, heat, radiation and vacuum. To study this, encoded protein clusters and pathways from an ongoing transcriptome study on the tardigrade Milnesium tardigradum were analyzed using bioinformatics tools and compared to expressed sequence tags (ESTs) from Hypsibius dujardini, revealing major pathways involved in resistance against extreme environmental conditions. ESTs are available on the Tardigrade Workbench along with software and databank updates. Our analysis reveals that RNA stability motifs for M. tardigradum are different from typical motifs known from higher animals. M. tardigradum and H. dujardini protein clusters and conserved domains imply metabolic storage pathways for glycogen, glycolipids and specific secondary metabolism as well as stress response pathways (including heat shock proteins, bmh2, and specific repair pathways). Redox-, DNA-, stress- and protein protection pathways complement specific repair capabilities to achieve the strong robustness of M. tardigradum. These pathways are partly conserved in other animals and their manipulation could boost stress adaptation even in human cells. However, the unique combination of resistance and repair pathways make tardigrades and M. tardigradum in particular so highly stress resistant.

  13. Stable isotope analysis of vertebrae reveals ontogenetic changes in habitat in an endothermic pelagic shark.

    Science.gov (United States)

    Carlisle, Aaron B; Goldman, Kenneth J; Litvin, Steven Y; Madigan, Daniel J; Bigman, Jennifer S; Swithenbank, Alan M; Kline, Thomas C; Block, Barbara A

    2015-01-22

    Ontogenetic changes in habitat are driven by shifting life-history requirements and play an important role in population dynamics. However, large portions of the life history of many pelagic species are still poorly understood or unknown. We used a novel combination of stable isotope analysis of vertebral annuli, Bayesian mixing models, isoscapes and electronic tag data to reconstruct ontogenetic patterns of habitat and resource use in a pelagic apex predator, the salmon shark (Lamna ditropis). Results identified the North Pacific Transition Zone as the major nursery area for salmon sharks and revealed an ontogenetic shift around the age of maturity from oceanic to increased use of neritic habitats. The nursery habitat may reflect trade-offs between prey availability, predation pressure and thermal constraints on juvenile endothermic sharks. The ontogenetic shift in habitat coincided with a reduction of isotopic niche, possibly reflecting specialization upon particular prey or habitats. Using tagging data to inform Bayesian isotopic mixing models revealed that adult sharks primarily use neritic habitats of Alaska yet receive a trophic subsidy from oceanic habitats. Integrating the multiple methods used here provides a powerful approach to retrospectively study the ecology and life history of migratory species throughout their ontogeny.

  14. Precursors of stall and surge processes in gas turbines revealed by wavelet analysis

    Energy Technology Data Exchange (ETDEWEB)

    Dremin, I.M.; Ivanov, O.V.; Nechitailo, V.A. [P.N. Lebedev Physical Institute, Moscow (Russian Federation); Furletov, V.I. [Central Institute for Aviation Motors, Moscow (Russian Federation); Terziev, V.G. [TEKO, Moscow (Russian Federation)

    2002-06-01

    Multiresolution wavelet analysis of pressure variations in a gas turbine compressor reveals the existence of precursors of stall and surge processes. Signals from eight pressure sensors positioned at various places within the compressor were recorded and digitized in three different operating modes in stationary conditions with a recording interval of 1 ms during 5-6 s. It has been discovered that there exists a scale of 32 intervals over which the dispersion (variance) of the wavelet coefficients shows a remarkable drop of about 40% for more than 1 s prior to the development of the malfunction. A shuffled sample of the same values of the pressure does not show such a drop demonstrating the dynamical origin of this effect. Higher order correlation moments reveal different slopes in these two regions differing by the variance values. The log-log dependence of the moments does not show clear fractal behavior because the scales of 16 and 32 intervals are not on the straight line of monofractals. This is a clear indication of the nonlinear response of the system at this scale. These results provide a means for automatic regulation of an engine, preventing possible failures. (author)

  15. Dichotomy of cellular inhibition by small-molecule inhibitors revealed by single-cell analysis

    Science.gov (United States)

    Vogel, Robert M.; Erez, Amir; Altan-Bonnet, Grégoire

    2016-01-01

    Despite progress in drug development, a quantitative and physiological understanding of how small-molecule inhibitors act on cells is lacking. Here, we measure the signalling and proliferative response of individual primary T-lymphocytes to a combination of antigen, cytokine and drug. We uncover two distinct modes of signalling inhibition: digital inhibition (the activated fraction of cells diminishes upon drug treatment, but active cells appear unperturbed), versus analogue inhibition (the activated fraction is unperturbed whereas activation response is diminished). We introduce a computational model of the signalling cascade that accounts for such inhibition dichotomy, and test the model predictions for the phenotypic variability of cellular responses. Finally, we demonstrate that the digital/analogue dichotomy of cellular response as revealed on short (signal transduction) timescales, translates into similar dichotomy on longer (proliferation) timescales. Our single-cell analysis of drug action illustrates the strength of quantitative approaches to translate in vitro pharmacology into functionally relevant cellular settings. PMID:27687249

  16. Conformational Dynamics of apo-GlnBP Revealed by Experimental and Computational Analysis

    KAUST Repository

    Feng, Yitao

    2016-10-13

    The glutamine binding protein (GlnBP) binds l-glutamine and cooperates with its cognate transporters during glutamine uptake. Crystal structure analysis has revealed an open and a closed conformation for apo- and holo-GlnBP, respectively. However, the detailed conformational dynamics have remained unclear. Herein, we combined NMR spectroscopy, MD simulations, and single-molecule FRET techniques to decipher the conformational dynamics of apo-GlnBP. The NMR residual dipolar couplings of apo-GlnBP were in good agreement with a MD-derived structure ensemble consisting of four metastable states. The open and closed conformations are the two major states. This four-state model was further validated by smFRET experiments and suggests the conformational selection mechanism in ligand recognition of GlnBP. © 2016 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim

  17. Bifidobacterium asteroides PRL2011 genome analysis reveals clues for colonization of the insect gut.

    Directory of Open Access Journals (Sweden)

    Francesca Bottacini

    Full Text Available Bifidobacteria are known as anaerobic/microaerophilic and fermentative microorganisms, which commonly inhabit the gastrointestinal tract of various animals and insects. Analysis of the 2,167,301 bp genome of Bifidobacterium asteroides PRL2011, a strain isolated from the hindgut of Apis mellifera var. ligustica, commonly known as the honey bee, revealed its predicted capability for respiratory metabolism. Conservation of the latter gene clusters in various B. asteroides strains enforces the notion that respiration is a common metabolic feature of this ancient bifidobacterial species, which has been lost in currently known mammal-derived Bifidobacterium species. In fact, phylogenomic based analyses suggested an ancient origin of B. asteroides and indicates it as an ancestor of the genus Bifidobacterium. Furthermore, the B. asteroides PRL2011 genome encodes various enzymes for coping with toxic products that arise as a result of oxygen-mediated respiration.

  18. Proteomic Analysis of Human Brown Adipose Tissue Reveals Utilization of Coupled and Uncoupled Energy Expenditure Pathways.

    Science.gov (United States)

    Müller, Sebastian; Balaz, Miroslav; Stefanicka, Patrik; Varga, Lukas; Amri, Ez-Zoubir; Ukropec, Jozef; Wollscheid, Bernd; Wolfrum, Christian

    2016-07-15

    Human brown adipose tissue (BAT) has become an attractive target to combat the current epidemical spread of obesity and its associated co-morbidities. Currently, information on its functional role is primarily derived from rodent studies. Here, we present the first comparative proteotype analysis of primary human brown adipose tissue versus adjacent white adipose tissue, which reveals significant quantitative differences in protein abundances and in turn differential functional capabilities. The majority of the 318 proteins with increased abundance in BAT are associated with mitochondrial metabolism and confirm the increased oxidative capacity. In addition to uncoupling protein 1 (UCP1), the main functional effector for uncoupled respiration, we also detected the mitochondrial creatine kinases (CKMT1A/B, CKMT2), as effective modulators of ATP synthase coupled respiration, to be exclusively expressed in BAT. The abundant expression and utilization of both energy expenditure pathways in parallel highlights the complex functional involvement of BAT in human physiology.

  19. Anomalous dispersion of Lagrangian particles in local regions of turbulent flows revealed by convex hull analysis

    CERN Document Server

    Pratt, J; Mueller, W -C; Chapman, S C; Watkins, N W

    2014-01-01

    Local regions of anomalous particle dispersion, and intermittent events that occur in turbulent flows can greatly influence the global statistical description of the flow. These local behaviors can be identified and analyzed by comparing the growth of neighboring convex hulls of Lagrangian tracer particles. Although in our simulations of homogeneous turbulence the convex hulls generally grow in size, after the Lagrangian particles that define the convex hulls begin to disperse, our analysis reveals short periods when the convex hulls of the Lagrangian particles shrink, evidence that particles are not dispersing simply. Shrinkage can be associated with anisotropic flows, since it occurs most frequently in the presence of a mean magnetic field or thermal convection. We compare dispersion between a wide range of statistically homogeneous and stationary turbulent flows ranging from homogeneous isotropic Navier-Stokes turbulence over different configurations of magnetohydrodynamic turbulence and Boussinesq convect...

  20. Network-based diffusion analysis reveals cultural transmission of lobtail feeding in humpback whales.

    Science.gov (United States)

    Allen, Jenny; Weinrich, Mason; Hoppitt, Will; Rendell, Luke

    2013-04-26

    We used network-based diffusion analysis to reveal the cultural spread of a naturally occurring foraging innovation, lobtail feeding, through a population of humpback whales (Megaptera novaeangliae) over a period of 27 years. Support for models with a social transmission component was 6 to 23 orders of magnitude greater than for models without. The spatial and temporal distribution of sand lance, a prey species, was also important in predicting the rate of acquisition. Our results, coupled with existing knowledge about song traditions, show that this species can maintain multiple independently evolving traditions in its populations. These insights strengthen the case that cetaceans represent a peak in the evolution of nonhuman culture, independent of the primate lineage.

  1. Bifidobacterium asteroides PRL2011 genome analysis reveals clues for colonization of the insect gut.

    Science.gov (United States)

    Bottacini, Francesca; Milani, Christian; Turroni, Francesca; Sánchez, Borja; Foroni, Elena; Duranti, Sabrina; Serafini, Fausta; Viappiani, Alice; Strati, Francesco; Ferrarini, Alberto; Delledonne, Massimo; Henrissat, Bernard; Coutinho, Pedro; Fitzgerald, Gerald F; Margolles, Abelardo; van Sinderen, Douwe; Ventura, Marco

    2012-01-01

    Bifidobacteria are known as anaerobic/microaerophilic and fermentative microorganisms, which commonly inhabit the gastrointestinal tract of various animals and insects. Analysis of the 2,167,301 bp genome of Bifidobacterium asteroides PRL2011, a strain isolated from the hindgut of Apis mellifera var. ligustica, commonly known as the honey bee, revealed its predicted capability for respiratory metabolism. Conservation of the latter gene clusters in various B. asteroides strains enforces the notion that respiration is a common metabolic feature of this ancient bifidobacterial species, which has been lost in currently known mammal-derived Bifidobacterium species. In fact, phylogenomic based analyses suggested an ancient origin of B. asteroides and indicates it as an ancestor of the genus Bifidobacterium. Furthermore, the B. asteroides PRL2011 genome encodes various enzymes for coping with toxic products that arise as a result of oxygen-mediated respiration.

  2. Metabolomic analysis reveals metabolic disturbance in the cortex and hippocampus of subchronic MK-801 treated rats.

    Directory of Open Access Journals (Sweden)

    Liya Sun

    Full Text Available BACKGROUND: Although a number of proteins and genes relevant to schizophrenia have been identified in recent years, few are known about the exact metabolic pathway involved in this disease. Our previous proteomic study has revealed the energy metabolism abnormality in subchronic MK-801 treated rat, a well-established animal model for schizophrenia. This prompted us to further investigate metabolite levels in the same rat model to better delineate the metabolism dysfunctions and provide insights into the pathology of schizophrenia. METHODS: Metabolomics, a high-throughput investigatory strategy developed in recent years, can offer comprehensive metabolite-level insights that complement protein and genetic findings. In this study, we employed a nondestructive metabolomic approach (1H-MAS-NMR to investigate the metabolic traits in cortex and hippocampus of MK-801 treated rats. Multivariate statistics and ingenuity pathways analyses (IPA were applied in data processing. The result was further integrated with our previous proteomic findings by IPA analysis to obtain a systematic view on our observations. RESULTS: Clear distinctions between the MK-801 treated group and the control group in both cortex and hippocampus were found by OPLS-DA models (with R(2X = 0.441, Q(2Y = 0.413 and R(2X = 0.698, Q(2Y = 0.677, respectively. The change of a series of metabolites accounted for the separation, such as glutamate, glutamine, citrate and succinate. Most of these metabolites fell in a pathway characterized by down-regulated glutamate synthesis and disturbed Krebs cycle. IPA analysis further confirmed the involvement of energy metabolism abnormality induced by MK-801 treatment. CONCLUSIONS: Our metabolomics findings reveal systematic changes in pathways of glutamate metabolism and Krebs cycle in the MK-801 treated rats' cortex and hippocampus, which confirmed and improved our previous proteomic observation and served as a valuable reference to

  3. Cluster analysis reveals a binary effect of storage on boar sperm motility function.

    Science.gov (United States)

    Henning, Heiko; Petrunkina, Anna M; Harrison, Robin A P; Waberski, Dagmar

    2014-06-01

    Storage of liquid-preserved boar spermatozoa is associated with a loss of fertilising ability of the preserved spermatozoa, which standard semen parameters barely reflect. Monitoring responses to molecular effectors of sperm function (e.g. bicarbonate) has proven to be a more sensitive approach to investigating storage effects. Bicarbonate not only initiates capacitation in spermatozoa, but also induces motility activation. This occurs at ejaculation, but also happens throughout passage through the oviduct. In the present study we tested whether the specific response of boar sperm subpopulations to bicarbonate, as assessed by motility activation, is altered with the duration of storage in vitro. Three ejaculates from each of seven boars were diluted in Beltsville thawing solution and stored at 17°C. Only minor changes in the parameters of diluted semen were revealed over a period of 72h storage. For assessment of bicarbonate responses, subsamples of diluted spermatozoa were centrifuged through a discontinuous Percoll gradient after 12, 24 and 72h storage. Subsequently, spermatozoa were incubated in two Ca2+-free variants of Tyrode's medium either without (TyrControl) or with (TyrBic) 15mM bicarbonate, and computer-aided sperm analysis motility measurements were made. Cluster analysis of imaging data from motile spermatozoa revealed the presence of five major sperm subpopulations with distinct motility characteristics, differing between TyrBic and TyrControl at any given time (Psperm motility function descriptors to storage: although the quantitative descriptor (percentage of motile spermatozoa) declines in washed semen samples, the qualitative descriptor (percentage of spermatozoa stimulated into fast linear motion by bicarbonate) is sustained independent of the duration of storage.

  4. Comparative transcriptional analysis reveals differential gene expression between asymmetric and symmetric zygotic divisions in tobacco.

    Directory of Open Access Journals (Sweden)

    Tian-Xiang Hu

    Full Text Available Asymmetric cell divisions occur widely during many developmental processes in plants. In most angiosperms, the first zygotic cell division is asymmetric resulting in two daughter cells of unequal size and with distinct fates. However, the critical molecular mechanisms regulating this division remain unknown. Previously we showed that treatment of tobacco zygotes with beta-glucosyl Yariv (βGlcY could dramatically alter the first zygotic asymmetric division to produce symmetric two-celled proembryos. In the present study, we isolated zygotes and two-celled asymmetric proembryos in vivo by micromanipulation, and obtained symmetric, two-celled proembryos by in vitro cell cultures. Using suppression-subtractive hybridization (SSH and macroarray analysis differential gene expression between the zygote and the asymmetric and symmetric two-celled proembryos was investigated. After sequencing of the differentially expressed clones, a total of 1610 EST clones representing 685 non-redundant transcripts were obtained. Gene ontology (GO term analysis revealed that these transcripts include those involved in physiological processes such as response to stimulus, regulation of gene expression, and localization and formation of anatomical structures. A homology search against known genes from Arabidopsis indicated that some of the above transcripts are involved in asymmetric cell division and embryogenesis. Quantitative real-time PCR confirmed the up- or down-regulation of the selected candidate transcripts during zygotic division. A few of these transcripts were expressed exclusively in the zygote, or in either type of the two-celled proembryos. Expression analyses of select genes in different tissues and organs also revealed potential roles of these transcripts in fertilization, seed maturation and organ development. The putative roles of few of the identified transcripts in the regulation of zygotic division are discussed. Further functional work on these

  5. Partial sequencing of the bottle gourd genome reveals markers useful for phylogenetic analysis and breeding

    Directory of Open Access Journals (Sweden)

    Wang Sha

    2011-09-01

    Full Text Available Abstract Background Bottle gourd [Lagenaria siceraria (Mol. Standl.] is an important cucurbit crop worldwide. Archaeological research indicates that bottle gourd was domesticated more than 10,000 years ago, making it one of the earliest plants cultivated by man. In spite of its widespread importance and long history of cultivation almost nothing has been known about the genome of this species thus far. Results We report here the partial sequencing of bottle gourd genome using the 454 GS-FLX Titanium sequencing platform. A total of 150,253 sequence reads, which were assembled into 3,994 contigs and 82,522 singletons were generated. The total length of the non-redundant singletons/assemblies is 32 Mb, theoretically covering ~ 10% of the bottle gourd genome. Functional annotation of the sequences revealed a broad range of functional types, covering all the three top-level ontologies. Comparison of the gene sequences between bottle gourd and the model cucurbit cucumber (Cucumis sativus revealed a 90% sequence similarity on average. Using the sequence information, 4395 microsatellite-containing sequences were identified and 400 SSR markers were developed, of which 94% amplified bands of anticipated sizes. Transferability of these markers to four other cucurbit species showed obvious decline with increasing phylogenetic distance. From analyzing polymorphisms of a subset of 14 SSR markers assayed on 44 representative China bottle gourd varieties/landraces, a principal coordinates (PCo analysis output and a UPGMA-based dendrogram were constructed. Bottle gourd accessions tended to group by fruit shape rather than geographic origin, although in certain subclades the lines from the same or close origin did tend to cluster. Conclusions This work provides an initial basis for genome characterization, gene isolation and comparative genomics analysis in bottle gourd. The SSR markers developed would facilitate marker assisted breeding schemes for efficient

  6. Numerical simulation on turbulent flow field in convergent-divergent nozzle

    Institute of Scientific and Technical Information of China (English)

    LU Yi-yu; LIO Yong; LI Xiao-hong; FANG Yong; ZHAO Jian-xin

    2009-01-01

    Because of the complication of turbulence's mechanism and law as well as the jet pressure in nozzle is difficult to test by experiment, five turbulent models were applied to numerically simulate the turbulent flow field in convergent-divergent nozzle. Theory analysis and experiment results of mass flow rates conclude that the RNG κ-ε model is the most suitable model. The pressure distribution in the convergent-divergent nozzle was revealed by computational fluid dynamic (CFD) simulating on the turbulent flow field under different pressure conditions. The growing conditions of cavitation bubbles were shown; meanwhile, the phenomena in the experiment could be explained. The differential pressure between the upstream and downstream in nozzle throat section can improve the cavitating effect of cavitation water jet.

  7. Convergent Evolution of Hemoglobin Function in High-Altitude Andean Waterfowl Involves Limited Parallelism at the Molecular Sequence Level.

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    Chandrasekhar Natarajan

    2015-12-01

    Full Text Available A fundamental question in evolutionary genetics concerns the extent to which adaptive phenotypic convergence is attributable to convergent or parallel changes at the molecular sequence level. Here we report a comparative analysis of hemoglobin (Hb function in eight phylogenetically replicated pairs of high- and low-altitude waterfowl taxa to test for convergence in the oxygenation properties of Hb, and to assess the extent to which convergence in biochemical phenotype is attributable to repeated amino acid replacements. Functional experiments on native Hb variants and protein engineering experiments based on site-directed mutagenesis revealed the phenotypic effects of specific amino acid replacements that were responsible for convergent increases in Hb-O2 affinity in multiple high-altitude taxa. In six of the eight taxon pairs, high-altitude taxa evolved derived increases in Hb-O2 affinity that were caused by a combination of unique replacements, parallel replacements (involving identical-by-state variants with independent mutational origins in different lineages, and collateral replacements (involving shared, identical-by-descent variants derived via introgressive hybridization. In genome scans of nucleotide differentiation involving high- and low-altitude populations of three separate species, function-altering amino acid polymorphisms in the globin genes emerged as highly significant outliers, providing independent evidence for adaptive divergence in Hb function. The experimental results demonstrate that convergent changes in protein function can occur through multiple historical paths, and can involve multiple possible mutations. Most cases of convergence in Hb function did not involve parallel substitutions and most parallel substitutions did not affect Hb-O2 affinity, indicating that the repeatability of phenotypic evolution does not require parallelism at the molecular level.

  8. Convergent Evolution of Hemoglobin Function in High-Altitude Andean Waterfowl Involves Limited Parallelism at the Molecular Sequence Level.

    Science.gov (United States)

    Natarajan, Chandrasekhar; Projecto-Garcia, Joana; Moriyama, Hideaki; Weber, Roy E; Muñoz-Fuentes, Violeta; Green, Andy J; Kopuchian, Cecilia; Tubaro, Pablo L; Alza, Luis; Bulgarella, Mariana; Smith, Matthew M; Wilson, Robert E; Fago, Angela; McCracken, Kevin G; Storz, Jay F

    2015-12-01

    A fundamental question in evolutionary genetics concerns the extent to which adaptive phenotypic convergence is attributable to convergent or parallel changes at the molecular sequence level. Here we report a comparative analysis of hemoglobin (Hb) function in eight phylogenetically replicated pairs of high- and low-altitude waterfowl taxa to test for convergence in the oxygenation properties of Hb, and to assess the extent to which convergence in biochemical phenotype is attributable to repeated amino acid replacements. Functional experiments on native Hb variants and protein engineering experiments based on site-directed mutagenesis revealed the phenotypic effects of specific amino acid replacements that were responsible for convergent increases in Hb-O2 affinity in multiple high-altitude taxa. In six of the eight taxon pairs, high-altitude taxa evolved derived increases in Hb-O2 affinity that were caused by a combination of unique replacements, parallel replacements (involving identical-by-state variants with independent mutational origins in different lineages), and collateral replacements (involving shared, identical-by-descent variants derived via introgressive hybridization). In genome scans of nucleotide differentiation involving high- and low-altitude populations of three separate species, function-altering amino acid polymorphisms in the globin genes emerged as highly significant outliers, providing independent evidence for adaptive divergence in Hb function. The experimental results demonstrate that convergent changes in protein function can occur through multiple historical paths, and can involve multiple possible mutations. Most cases of convergence in Hb function did not involve parallel substitutions and most parallel substitutions did not affect Hb-O2 affinity, indicating that the repeatability of phenotypic evolution does not require parallelism at the molecular level.

  9. Comparative transcriptomic analysis reveals similarities and dissimilarities in Saccharomyces cerevisiae wine strains response to nitrogen availability.

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    Catarina Barbosa

    Full Text Available Nitrogen levels in grape-juices are of major importance in winemaking ensuring adequate yeast growth and fermentation performance. Here we used a comparative transcriptome analysis to uncover wine yeasts responses to nitrogen availability during fermentation. Gene expression was assessed in three genetically and phenotypically divergent commercial wine strains (CEG, VL1 and QA23, under low (67 mg/L and high nitrogen (670 mg/L regimes, at three time points during fermentation (12 h, 24 h and 96 h. Two-way ANOVA analysis of each fermentation condition led to the identification of genes whose expression was dependent on strain, fermentation stage and on the interaction of both factors. The high fermenter yeast strain QA23 was more clearly distinct from the other two strains, by differential expression of genes involved in flocculation, mitochondrial functions, energy generation and protein folding and stabilization. For all strains, higher transcriptional variability due to fermentation stage was seen in the high nitrogen fermentations. A positive correlation between maximum fermentation rate and the expression of genes involved in stress response was observed. The finding of common genes correlated with both fermentation activity and nitrogen up-take underlies the role of nitrogen on yeast fermentative fitness. The comparative analysis of genes differentially expressed between both fermentation conditions at 12 h, where the main difference was the level of nitrogen available, showed the highest variability amongst strains revealing strain-specific responses. Nevertheless, we were able to identify a small set of genes whose expression profiles can quantitatively assess the common response of the yeast strains to varying nitrogen conditions. The use of three contrasting yeast strains in gene expression analysis prompts the identification of more reliable, accurate and reproducible biomarkers that will facilitate the diagnosis of deficiency of this

  10. Cosmological constraints from the convergence 1-point probability distribution

    CERN Document Server

    Patton, Kenneth; Honscheid, Klaus; Huff, Eric; Melchior, Peter; Ross, Ashley J; Suchyta, Eric

    2016-01-01

    We examine the cosmological information available from the 1-point probability distribution (PDF) of the weak-lensing convergence field, utilizing fast L-PICOLA simulations and a Fisher analysis. We find competitive constraints in the $\\Omega_m$-$\\sigma_8$ plane from the convergence PDF with $188\\ arcmin^2$ pixels compared to the cosmic shear power spectrum with an equivalent number of modes ($\\ell < 886$). The convergence PDF also partially breaks the degeneracy cosmic shear exhibits in that parameter space. A joint analysis of the convergence PDF and shear 2-point function also reduces the impact of shape measurement systematics, to which the PDF is less susceptible, and improves the total figure of merit by a factor of $2-3$, depending on the level of systematics. Finally, we present a correction factor necessary for calculating the unbiased Fisher information from finite differences using a limited number of cosmological simulations.

  11. The anticipation of converging industries a concept applied to nutraceuticals and functional foods

    CERN Document Server

    Curran, Clive-Steven

    2013-01-01

    The blurring of boundaries between hitherto distinct scientific disciplines, technologies or markets is a common and powerful phenomenon. Subjects of this convergence often change consumer behaviours, favouring products and platforms with multiple functions. The Anticipation of Converging Industries provides a detailed focus on the triggers, drivers and consequences of convergence to create a more concise definition of convergence.   This detailed analysis includes a specifically developed toolbox for ‘convergence foresight’, creating a forecasting method for convergence trends. With the focus on the chemical, biotechnological and pharmaceutical industries, several indicators of convergence in the areas of Nutraceuticals/Functional Foods, Cosmeceuticals and ICT are derived from samples including over 1million patents and scientific publications.   By supporting this methodical approach with real world data, The Anticipation of Converging Industries is perfect for industry practitioners looking for a com...

  12. On the non-convergence of energy intensities. Evidence from a pair-wise econometric approach

    Energy Technology Data Exchange (ETDEWEB)

    Le Pen, Yannick [Universite de Nantes LEMNA, Nantes (France); Sevi, Benoit [Universite d' Angers GRANEM, Faculte de Droit, Economie et Gestion, Universite d' Angers, 13 allee Francois Mitterrand, BP 13633, 49036 Angers cedex 01 (France)

    2010-01-15

    This paper evaluates the convergence of energy intensities for a group of 97 countries in the period 1971-2003. Convergence is tested using a recent method proposed by Pesaran (2007) [Pesaran, M.H., 2007. A pair-wise approach to testing for output and growth convergence. Journal of Econometrics 138, 312-355] based on the stochastic convergence criterion. An advantage of this method is that results do not depend on a benchmark against which convergence is assessed. It gives more robust results. Applications of several unit-root tests as well as a stationarity test uniformly reject the global convergence hypothesis. Locally, for Middle East, OECD and Europe sub-groups, non-convergence is less strongly rejected. The introduction of possible structural breaks in the analysis only marginally provides more support to the convergence hypothesis. (author)

  13. Digital Convergence and Content Regulation

    Directory of Open Access Journals (Sweden)

    Michael John Starks

    2014-12-01

    Full Text Available Broadcasting, Press and Internet journalism systems of distribution are converging: the same infrastructure can deliver all three historically separate services. Reception devices mirror this: the Connected TV, the tablet and the smart phone overlap in their functionality. Service overlaps are evident too, with broadcasters providing online and on-demand services and newspapers developing electronic versions. Does this mean that media regulation policies must converge too?My argument is that they should, though only where historically different communications are now fulfilling a similar function, e.g. broadcaster online services and electronic versions of newspapers. Convergence requires a degree of harmonisation and, to this end, I advocate a review of UK broadcasting's 'due impartiality' requirement and of the UK's application of the public service concept. I also argue for independent self-regulation (rather than state-based regulation of non-public-service broadcasting journalism.

  14. Order Handling in Convergent Environments

    CERN Document Server

    Vrtanoski, Jordan

    2012-01-01

    The rapid development of IT&T technology had big impact on the traditional telecommunications market, transforming it from monopolistic market to highly competitive high-tech market where new services are required to be created frequently. This paper aims to describe a design approach that puts order management process (as part of enterprise application integration) in function of rapid service creation. In the text we will present a framework for collaborative order handling supporting convergent services. The design splits the order handling processes in convergent environments in three business process groups: order capture, order management and order fulfillment. The paper establishes abstract framework for order handling and provides design guidelines for transaction handling implementation based on the checkpoint and inverse command strategy. The proposed design approach is based in a convergent telecommunication environment. Same principles are applicable in solving problems of collaboration in fun...

  15. Convergence of Composition Operators on Hardy-Smirnov Space

    Institute of Scientific and Technical Information of China (English)

    Xiang Dong YANG

    2011-01-01

    We consider the convergence of composition operators on Hardy-Smirnov space over a simply connected domain properly contained in the complex plane.The convergence of the power of a composition operator is also considered.Our approach is a method from Joel H.Shapiro and Wayne Smith's celebrated work (Journal of Functional Analysis 205 (2003) 62-89).The resulting space is usually not the one obtained from the classical Hardy space of the unit disc by conformal mapping.

  16. Automated image analysis reveals the dynamic 3-dimensional organization of multi-ciliary arrays.

    Science.gov (United States)

    Galati, Domenico F; Abuin, David S; Tauber, Gabriel A; Pham, Andrew T; Pearson, Chad G

    2015-12-23

    Multi-ciliated cells (MCCs) use polarized fields of undulating cilia (ciliary array) to produce fluid flow that is essential for many biological processes. Cilia are positioned by microtubule scaffolds called basal bodies (BBs) that are arranged within a spatially complex 3-dimensional geometry (3D). Here, we develop a robust and automated computational image analysis routine to quantify 3D BB organization in the ciliate, Tetrahymena thermophila. Using this routine, we generate the first morphologically constrained 3D reconstructions of Tetrahymena cells and elucidate rules that govern the kinetics of MCC organization. We demonstrate the interplay between BB duplication and cell size expansion through the cell cycle. In mutant cells, we identify a potential BB surveillance mechanism that balances large gaps in BB spacing by increasing the frequency of closely spaced BBs in other regions of the cell. Finally, by taking advantage of a mutant predisposed to BB disorganization, we locate the spatial domains that are most prone to disorganization by environmental stimuli. Collectively, our analyses reveal the importance of quantitative image analysis to understand the principles that guide the 3D organization of MCCs.

  17. Pre-2014 mudslides at Oso revealed by InSAR and multi-source DEM analysis

    Science.gov (United States)

    Kim, J. W.; Lu, Z.; QU, F.

    2014-12-01

    The landslide is a process that results in the downward and outward movement of slope-reshaping materials including rocks and soils and annually causes the loss of approximately $3.5 billion and tens of casualties in the United States. The 2014 Oso mudslide was an extreme event costing nearly 40 deaths and damaging civilian properties. Landslides are often unpredictable, but in many cases, catastrophic events are repetitive. Historic record in the Oso mudslide site indicates that there have been serial events in decades, though the extent of sliding events varied from time to time. In our study, the combination of multi-source DEMs, InSAR, and time-series InSAR analysis has enabled to characterize the Oso mudslide. InSAR results from ALOS PALSAR show that there was no significant deformation between mid-2006 and 2011. The combination of time-series InSAR analysis and old-dated DEM indicated revealed topographic changes associated the 2006 sliding event, which is confirmed by the difference of multiple LiDAR DEMs. Precipitation and discharge measurements before the 2006 and 2014 landslide events did not exhibit extremely anomalous records, suggesting the precipitation is not the controlling factor in determining the sliding events at Oso. The lack of surface deformation during 2006-2011 and weak correlation between the precipitation and the sliding event, suggest other factors (such as porosity) might play a critical role on the run-away events at this Oso and other similar landslides.

  18. Large-scale analysis by SAGE reveals new mechanisms of v-erbA oncogene action

    Directory of Open Access Journals (Sweden)

    Faure Claudine

    2007-10-01

    Full Text Available Abstract Background: The v-erbA oncogene, carried by the Avian Erythroblastosis Virus, derives from the c-erbAα proto-oncogene that encodes the nuclear receptor for triiodothyronine (T3R. v-ErbA transforms erythroid progenitors in vitro by blocking their differentiation, supposedly by interference with T3R and RAR (Retinoic Acid Receptor. However, v-ErbA target genes involved in its transforming activity still remain to be identified. Results: By using Serial Analysis of Gene Expression (SAGE, we identified 110 genes deregulated by v-ErbA and potentially implicated in the transformation process. Bioinformatic analysis of promoter sequence and transcriptional assays point out a potential role of c-Myb in the v-ErbA effect. Furthermore, grouping of newly identified target genes by function revealed both expected (chromatin/transcription and unexpected (protein metabolism functions potentially deregulated by v-ErbA. We then focused our study on 15 of the new v-ErbA target genes and demonstrated by real time PCR that in majority their expression was activated neither by T3, nor RA, nor during differentiation. This was unexpected based upon the previously known role of v-ErbA. Conclusion: This paper suggests the involvement of a wealth of new unanticipated mechanisms of v-ErbA action.

  19. Transcriptome analysis reveals dynamic changes in the gene expression of tobacco seedlings under low potassium stress

    Indian Academy of Sciences (India)

    Liming Lu; Yong Chen; Lin Lu; Yifei Lu; Liqin Li

    2015-09-01

    Potassium plays a key role in plant development and reproduction. In agricultural practice, potassium deficiency is common worldwide, and leads to crop growth inhibition and output reduction. In this study, we analysed the transcriptome of tobacco seedlings under low potassium stress. Tobacco seedlings with or without decreased potassium treatment were harvested after 0 (control), 6, 12, or 24 h and were submitted for microarray analysis. The results showed that up to 3790 genes were upregulated or downregulated more than 2-fold as a result of the decreased potassium treatment. Gene ontology analysis revealed significantly differentially expressed genes that were categorized as cation binding, transcription regulation, metabolic processes, transporter activity and enzyme regulation. Some potassium, nitrogen and phosphorus transporters; transcription factors; and plant signal molecules, such as CPKs were also significantly differentially expressed under potassium deficiency. Our results indicate that the expression profiles of a large number of genes involved in various plant physiological processes are significantly altered in response to potassium deficiency, which can result in physiological and morphological changes in tobacco plants.

  20. Bach Is the Father of Harmony: Revealed by a 1/f Fluctuation Analysis across Musical Genres.

    Directory of Open Access Journals (Sweden)

    Dan Wu

    Full Text Available Harmony is a fundamental attribute of music. Close connections exist between music and mathematics since both pursue harmony and unity. In music, the consonance of notes played simultaneously partly determines our perception of harmony; associates with aesthetic responses; and influences the emotion expression. The consonance could be considered as a window to understand and analyze harmony. Here for the first time we used a 1/f fluctuation analysis to investigate whether the consonance fluctuation structure in music with a wide range of composers and genres followed the scale free pattern that has been found for pitch, melody, rhythm, human body movements, brain activity, natural images and geographical features. We then used a network graph approach to investigate which composers were the most influential both within and across genres. Our results showed that patterns of consonance in music did follow scale-free characteristics, suggesting that this feature is a universally evolved one in both music and the living world. Furthermore, our network analysis revealed that Bach's harmony patterns were having the most influence on those used by other composers, followed closely by Mozart.

  1. Comparative Transcriptome Analysis Reveals Different Silk Yields of Two Silkworm Strains.

    Directory of Open Access Journals (Sweden)

    Juan Li

    Full Text Available Cocoon and silk yields are the most important characteristics of sericulture. However, few studies have examined the genes that modulate these features. Further studies of these genes will be useful for improving the products of sericulture. JingSong (JS and Lan10 (L10 are two strains having significantly different cocoon and silk yields. In the current study, RNA-Seq and quantitative polymerase chain reaction (qPCR were performed on both strains in order to determine divergence of the silk gland, which controls silk biosynthesis in silkworms. Compared with L10, JS had 1375 differentially expressed genes (DEGs; 738 up-regulated genes and 673 down-regulated genes. Nine enriched gene ontology (GO terms were identified by GO enrichment analysis based on these DEGs. KEGG enrichment analysis results showed that the DEGs were enriched in three pathways, which were mainly associated with the processing and biosynthesis of proteins. The representative genes in the enrichment pathways and ten significant DEGs were further verified by qPCR, the results of which were consistent with the RNA-Seq data. Our study has revealed differences in silk glands between the two silkworm strains and provides a perspective for understanding the molecular mechanisms determining silk yield.

  2. Comparative Genomic Analysis of Lactococcus garvieae Strains Isolated from Different Sources Reveals Candidate Virulence Genes

    Directory of Open Access Journals (Sweden)

    Eiji Miyauchi

    2012-01-01

    Full Text Available Lactococcus garvieae is a major pathogen for fish. Two complete (ATCC 49156 and Lg2 and three draft (UNIUD074, 8831, and 21881 genome sequences of L. garvieae have recently been released. We here present the results of a comparative genomic analysis of these fish and human isolates of L. garvieae. The pangenome comprised 1,542 core and 1,378 dispensable genes. The sequenced L. garvieae strains shared most of the possible virulence genes, but the capsule gene cluster was found only in fish-pathogenic strain Lg2. The absence of the capsule gene cluster in other nonpathogenic strains isolated from mastitis and vegetable was also confirmed by PCR. The fish and human isolates of L. garvieae contained the specific two and four adhesin genes, respectively, indicating that these adhesion proteins may be involved in the host specificity differences of L. garvieae. The discoveries revealed by the pangenomic analysis may provide significant insights into the biology of L. garvieae.

  3. Metagenomic analysis reveals that bacteriophages are reservoirs of antibiotic resistance genes.

    Science.gov (United States)

    Subirats, Jéssica; Sànchez-Melsió, Alexandre; Borrego, Carles M; Balcázar, José Luis; Simonet, Pascal

    2016-08-01

    A metagenomics approach was applied to explore the presence of antibiotic resistance genes (ARGs) in bacteriophages from hospital wastewater. Metagenomic analysis showed that most phage sequences affiliated to the order Caudovirales, comprising the tailed phage families Podoviridae, Siphoviridae and Myoviridae. Moreover, the relative abundance of ARGs in the phage DNA fraction (0.26%) was higher than in the bacterial DNA fraction (0.18%). These differences were particularly evident for genes encoding ATP-binding cassette (ABC) and resistance-nodulation-cell division (RND) proteins, phosphotransferases, β-lactamases and plasmid-mediated quinolone resistance. Analysis of assembled contigs also revealed that blaOXA-10, blaOXA-58 and blaOXA-24 genes belonging to class D β-lactamases as well as a novel blaTEM (98.9% sequence similarity to the blaTEM-1 gene) belonging to class A β-lactamases were detected in a higher proportion in phage DNA. Although preliminary, these findings corroborate the role of bacteriophages as reservoirs of resistance genes and thus highlight the necessity to include them in future studies on the emergence and spread of antibiotic resistance in the environment.

  4. Glycoproteomic Analysis of Seven Major Allergenic Proteins Reveals Novel Post-translational Modifications*

    Science.gov (United States)

    Halim, Adnan; Carlsson, Michael C.; Madsen, Caroline Benedicte; Brand, Stephanie; Møller, Svenning Rune; Olsen, Carl Erik; Vakhrushev, Sergey Y.; Brimnes, Jens; Wurtzen, Peter Adler; Ipsen, Henrik; Petersen, Bent L.; Wandall, Hans H.

    2015-01-01

    Allergenic proteins such as grass pollen and house dust mite (HDM) proteins are known to trigger hypersensitivity reactions of the immune system, leading to what is commonly known as allergy. Key allergenic proteins including sequence variants have been identified but characterization of their post-translational modifications (PTMs) is still limited. Here, we present a detailed PTM1 characterization of a series of the main and clinically relevant allergens used in allergy tests and vaccines. We employ Orbitrap-based mass spectrometry with complementary fragmentation techniques (HCD/ETD) for site-specific PTM characterization by bottom-up analysis. In addition, top-down mass spectrometry is utilized for targeted analysis of individual proteins, revealing hitherto unknown PTMs of HDM allergens. We demonstrate the presence of lysine-linked polyhexose glycans and asparagine-linked N-acetylhexosamine glycans on HDM allergens. Moreover, we identified more complex glycan structures than previously reported on the major grass pollen group 1 and 5 allergens, implicating important roles for carbohydrates in allergen recognition and response by the immune system. The new findings are important for understanding basic disease-causing mechanisms at the cellular level, which ultimately may pave the way for instigating novel approaches for targeted desensitization strategies and improved allergy vaccines. PMID:25389185

  5. Glycoproteomic analysis of seven major allergenic proteins reveals novel post-translational modifications.

    Science.gov (United States)

    Halim, Adnan; Carlsson, Michael C; Madsen, Caroline Benedicte; Brand, Stephanie; Møller, Svenning Rune; Olsen, Carl Erik; Vakhrushev, Sergey Y; Brimnes, Jens; Wurtzen, Peter Adler; Ipsen, Henrik; Petersen, Bent L; Wandall, Hans H

    2015-01-01

    Allergenic proteins such as grass pollen and house dust mite (HDM) proteins are known to trigger hypersensitivity reactions of the immune system, leading to what is commonly known as allergy. Key allergenic proteins including sequence variants have been identified but characterization of their post-translational modifications (PTMs) is still limited. Here, we present a detailed PTM(1) characterization of a series of the main and clinically relevant allergens used in allergy tests and vaccines. We employ Orbitrap-based mass spectrometry with complementary fragmentation techniques (HCD/ETD) for site-specific PTM characterization by bottom-up analysis. In addition, top-down mass spectrometry is utilized for targeted analysis of individual proteins, revealing hitherto unknown PTMs of HDM allergens. We demonstrate the presence of lysine-linked polyhexose glycans and asparagine-linked N-acetylhexosamine glycans on HDM allergens. Moreover, we identified more complex glycan structures than previously reported on the major grass pollen group 1 and 5 allergens, implicating important roles for carbohydrates in allergen recognition and response by the immune system. The new findings are important for understanding basic disease-causing mechanisms at the cellular level, which ultimately may pave the way for instigating novel approaches for targeted desensitization strategies and improved allergy vaccines.

  6. Independent component analysis of DTI data reveals white matter covariances in Alzheimer's disease

    Science.gov (United States)

    Ouyang, Xin; Sun, Xiaoyu; Guo, Ting; Sun, Qiaoyue; Chen, Kewei; Yao, Li; Wu, Xia; Guo, Xiaojuan

    2014-03-01

    Alzheimer's disease (AD) is a progressive neurodegenerative disease with the clinical symptom of the continuous deterioration of cognitive and memory functions. Multiple diffusion tensor imaging (DTI) indices such as fractional anisotropy (FA) and mean diffusivity (MD) can successfully explain the white matter damages in AD patients. However, most studies focused on the univariate measures (voxel-based analysis) to examine the differences between AD patients and normal controls (NCs). In this investigation, we applied a multivariate independent component analysis (ICA) to investigate the white matter covariances based on FA measurement from DTI data in 35 AD patients and 45 NCs from the Alzheimer's Disease Neuroimaging Initiative (ADNI) database. We found that six independent components (ICs) showed significant FA reductions in white matter covariances in AD compared with NC, including the genu and splenium of corpus callosum (IC-1 and IC-2), middle temporal gyral of temporal lobe (IC-3), sub-gyral of frontal lobe (IC-4 and IC-5) and sub-gyral of parietal lobe (IC-6). Our findings revealed covariant white matter loss in AD patients and suggest that the unsupervised data-driven ICA method is effective to explore the changes of FA in AD. This study assists us in understanding the mechanism of white matter covariant reductions in the development of AD.

  7. Bach Is the Father of Harmony: Revealed by a 1/f Fluctuation Analysis across Musical Genres.

    Science.gov (United States)

    Wu, Dan; Kendrick, Keith M; Levitin, Daniel J; Li, Chaoyi; Yao, Dezhong

    2015-01-01

    Harmony is a fundamental attribute of music. Close connections exist between music and mathematics since both pursue harmony and unity. In music, the consonance of notes played simultaneously partly determines our perception of harmony; associates with aesthetic responses; and influences the emotion expression. The consonance could be considered as a window to understand and analyze harmony. Here for the first time we used a 1/f fluctuation analysis to investigate whether the consonance fluctuation structure in music with a wide range of composers and genres followed the scale free pattern that has been found for pitch, melody, rhythm, human body movements, brain activity, natural images and geographical features. We then used a network graph approach to investigate which composers were the most influential both within and across genres. Our results showed that patterns of consonance in music did follow scale-free characteristics, suggesting that this feature is a universally evolved one in both music and the living world. Furthermore, our network analysis revealed that Bach's harmony patterns were having the most influence on those used by other composers, followed closely by Mozart.

  8. Analysis of spatial-temporal gene expression patterns reveals dynamics and regionalization in developing mouse brain.

    Science.gov (United States)

    Chou, Shen-Ju; Wang, Chindi; Sintupisut, Nardnisa; Niou, Zhen-Xian; Lin, Chih-Hsu; Li, Ker-Chau; Yeang, Chen-Hsiang

    2016-01-20

    Allen Brain Atlas (ABA) provides a valuable resource of spatial/temporal gene expressions in mammalian brains. Despite rich information extracted from this database, current analyses suffer from several limitations. First, most studies are either gene-centric or region-centric, thus are inadequate to capture the superposition of multiple spatial-temporal patterns. Second, standard tools of expression analysis such as matrix factorization can capture those patterns but do not explicitly incorporate spatial dependency. To overcome those limitations, we proposed a computational method to detect recurrent patterns in the spatial-temporal gene expression data of developing mouse brains. We demonstrated that regional distinction in brain development could be revealed by localized gene expression patterns. The patterns expressed in the forebrain, medullary and pontomedullary, and basal ganglia are enriched with genes involved in forebrain development, locomotory behavior, and dopamine metabolism respectively. In addition, the timing of global gene expression patterns reflects the general trends of molecular events in mouse brain development. Furthermore, we validated functional implications of the inferred patterns by showing genes sharing similar spatial-temporal expression patterns with Lhx2 exhibited differential expression in the embryonic forebrains of Lhx2 mutant mice. These analysis outcomes confirm the utility of recurrent expression patterns in studying brain development.

  9. Integrative microbial community analysis reveals full-scale enhanced biological phosphorus removal under tropical conditions

    Science.gov (United States)

    Law, Yingyu; Kirkegaard, Rasmus Hansen; Cokro, Angel Anisa; Liu, Xianghui; Arumugam, Krithika; Xie, Chao; Stokholm-Bjerregaard, Mikkel; Drautz-Moses, Daniela I.; Nielsen, Per Halkjær; Wuertz, Stefan; Williams, Rohan B. H.

    2016-05-01

    Management of phosphorus discharge from human waste is essential for the control of eutrophication in surface waters. Enhanced biological phosphorus removal (EBPR) is a sustainable, efficient way of removing phosphorus from waste water without employing chemical precipitation, but is assumed unachievable in tropical temperatures due to conditions that favour glycogen accumulating organisms (GAOs) over polyphosphate accumulating organisms (PAOs). Here, we show these assumptions are unfounded by studying comparative community dynamics in a full-scale plant following systematic perturbation of operational conditions, which modified community abundance, function and physicochemical state. A statistically significant increase in the relative abundance of the PAO Accumulibacter was associated with improved EBPR activity. GAO relative abundance also increased, challenging the assumption of competition. An Accumulibacter bin-genome was identified from a whole community metagenomic survey, and comparative analysis against extant Accumulibacter genomes suggests a close relationship to Type II. Analysis of the associated metatranscriptome data revealed that genes encoding proteins involved in the tricarboxylic acid cycle and glycolysis pathways were highly expressed, consistent with metabolic modelling results. Our findings show that tropical EBPR is indeed possible, highlight the translational potential of studying competition dynamics in full-scale waste water communities and carry implications for plant design in tropical regions.

  10. Comprehensive Proteomics Analysis of Laticifer Latex Reveals New Insights into Ethylene Stimulation of Natural Rubber Production.

    Science.gov (United States)

    Wang, Xuchu; Wang, Dan; Sun, Yong; Yang, Qian; Chang, Lili; Wang, Limin; Meng, Xueru; Huang, Qixing; Jin, Xiang; Tong, Zheng

    2015-09-08

    Ethylene is a stimulant to increase natural rubber latex. After ethylene application, both fresh yield and dry matter of latex are substantially improved. Moreover, we found that ethylene improves the generation of small rubber particles. However, most genes involved in rubber biosynthesis are inhibited by exogenous ethylene. Therefore, we conducted a proteomics analysis of ethylene-stimulated rubber latex, and identified 287 abundant proteins as well as 143 ethylene responsive latex proteins (ERLPs) with mass spectrometry from the 2-DE and DIGE gels, respectively. In addition, more than 1,600 proteins, including 404 ERLPs, were identified by iTRAQ. Functional classification of ERLPs revealed that enzymes involved in post-translational modification, carbohydrate metabolism, hydrolase activity, and kinase activity were overrepresented. Some enzymes for rubber particle aggregation were inhibited to prolong latex flow, and thus finally improved latex production. Phosphoproteomics analysis identified 59 differential phosphoproteins; notably, specific isoforms of rubber elongation factor and small rubber particle protein that were phosphorylated mainly at serine residues. This post-translational modification and isoform-specific phosphorylation might be important for ethylene-stimulated latex production. These results not only deepen our understanding of the rubber latex proteome but also provide new insights into the use of ethylene to stimulate rubber latex production.

  11. Meta-analysis of Dense Genecentric Association Studies Reveals Common and Uncommon Variants Associated with Height

    Science.gov (United States)

    Lanktree, Matthew B.; Guo, Yiran; Murtaza, Muhammed; Glessner, Joseph T.; Bailey, Swneke D.; Onland-Moret, N. Charlotte; Lettre, Guillaume; Ongen, Halit; Rajagopalan, Ramakrishnan; Johnson, Toby; Shen, Haiqing; Nelson, Christopher P.; Klopp, Norman; Baumert, Jens; Padmanabhan, Sandosh; Pankratz, Nathan; Pankow, James S.; Shah, Sonia; Taylor, Kira; Barnard, John; Peters, Bas J.; M. Maloney, Cliona; Lobmeyer, Maximilian T.; Stanton, Alice; Zafarmand, M. Hadi; Romaine, Simon P.R.; Mehta, Amar; van Iperen, Erik P.A.; Gong, Yan; Price, Tom S.; Smith, Erin N.; Kim, Cecilia E.; Li, Yun R.; Asselbergs, Folkert W.; Atwood, Larry D.; Bailey, Kristian M.; Bhatt, Deepak; Bauer, Florianne; Behr, Elijah R.; Bhangale, Tushar; Boer, Jolanda M.A.; Boehm, Bernhard O.; Bradfield, Jonathan P.; Brown, Morris; Braund, Peter S.; Burton, Paul R.; Carty, Cara; Chandrupatla, Hareesh R.; Chen, Wei; Connell, John; Dalgeorgou, Chrysoula; Boer, Anthonius de; Drenos, Fotios; Elbers, Clara C.; Fang, James C.; Fox, Caroline S.; Frackelton, Edward C.; Fuchs, Barry; Furlong, Clement E.; Gibson, Quince; Gieger, Christian; Goel, Anuj; Grobbee, Diederik E.; Hastie, Claire; Howard, Philip J.; Huang, Guan-Hua; Johnson, W. Craig; Li, Qing; Kleber, Marcus E.; Klein, Barbara E.K.; Klein, Ronald; Kooperberg, Charles; Ky, Bonnie; LaCroix, Andrea; Lanken, Paul; Lathrop, Mark; Li, Mingyao; Marshall, Vanessa; Melander, Olle; Mentch, Frank D.; J. Meyer, Nuala; Monda, Keri L.; Montpetit, Alexandre; Murugesan, Gurunathan; Nakayama, Karen; Nondahl, Dave; Onipinla, Abiodun; Rafelt, Suzanne; Newhouse, Stephen J.; Otieno, F. George; Patel, Sanjey R.; Putt, Mary E.; Rodriguez, Santiago; Safa, Radwan N.; Sawyer, Douglas B.; Schreiner, Pamela J.; Simpson, Claire; Sivapalaratnam, Suthesh; Srinivasan, Sathanur R.; Suver, Christine; Swergold, Gary; Sweitzer, Nancy K.; Thomas, Kelly A.; Thorand, Barbara; Timpson, Nicholas J.; Tischfield, Sam; Tobin, Martin; Tomaszweski, Maciej; Verschuren, W.M. Monique; Wallace, Chris; Winkelmann, Bernhard; Zhang, Haitao; Zheng, Dongling; Zhang, Li; Zmuda, Joseph M.; Clarke, Robert; Balmforth, Anthony J.; Danesh, John; Day, Ian N.; Schork, Nicholas J.; de Bakker, Paul I.W.; Delles, Christian; Duggan, David; Hingorani, Aroon D.; Hirschhorn, Joel N.; Hofker, Marten H.; Humphries, Steve E.; Kivimaki, Mika; Lawlor, Debbie A.; Kottke-Marchant, Kandice; Mega, Jessica L.; Mitchell, Braxton D.; Morrow, David A.; Palmen, Jutta; Redline, Susan; Shields, Denis C.; Shuldiner, Alan R.; Sleiman, Patrick M.; Smith, George Davey; Farrall, Martin; Jamshidi, Yalda; Christiani, David C.; Casas, Juan P.; Hall, Alistair S.; Doevendans, Pieter A.; D. Christie, Jason; Berenson, Gerald S.; Murray, Sarah S.; Illig, Thomas; Dorn, Gerald W.; Cappola, Thomas P.; Boerwinkle, Eric; Sever, Peter; Rader, Daniel J.; Reilly, Muredach P.; Caulfield, Mark; Talmud, Philippa J.; Topol, Eric; Engert, James C.; Wang, Kai; Dominiczak, Anna; Hamsten, Anders; Curtis, Sean P.; Silverstein, Roy L.; Lange, Leslie A.; Sabatine, Marc S.; Trip, Mieke; Saleheen, Danish; Peden, John F.; Cruickshanks, Karen J.; März, Winfried; O'Connell, Jeffrey R.; Klungel, Olaf H.; Wijmenga, Cisca; Maitland-van der Zee, Anke Hilse; Schadt, Eric E.; Johnson, Julie A.; Jarvik, Gail P.; Papanicolaou, George J.; Grant, Struan F.A.; Munroe, Patricia B.; North, Kari E.; Samani, Nilesh J.; Koenig, Wolfgang; Gaunt, Tom R.; Anand, Sonia S.; van der Schouw, Yvonne T.; Soranzo, Nicole; FitzGerald, Garret A.; Reiner, Alex; Hegele, Robert A.; Hakonarson, Hakon; Keating, Brendan J.

    2011-01-01

    Height is a classic complex trait with common variants in a growing list of genes known to contribute to the phenotype. Using a genecentric genotyping array targeted toward cardiovascular-related loci, comprising 49,320 SNPs across approximately 2000 loci, we evaluated the association of common and uncommon SNPs with adult height in 114,223 individuals from 47 studies and six ethnicities. A total of 64 loci contained a SNP associated with height at array-wide significance (p < 2.4 × 10−6), with 42 loci surpassing the conventional genome-wide significance threshold (p < 5 × 10−8). Common variants with minor allele frequencies greater than 5% were observed to be associated with height in 37 previously reported loci. In individuals of European ancestry, uncommon SNPs in IL11 and SMAD3, which would not be genotyped with the use of standard genome-wide genotyping arrays, were strongly associated with height (p < 3 × 10−11). Conditional analysis within associated regions revealed five additional variants associated with height independent of lead SNPs within the locus, suggesting allelic heterogeneity. Although underpowered to replicate findings from individuals of European ancestry, the direction of effect of associated variants was largely consistent in African American, South Asian, and Hispanic populations. Overall, we show that dense coverage of genes for uncommon SNPs, coupled with large-scale meta-analysis, can successfully identify additional variants associated with a common complex trait. PMID:21194676

  12. Structure analysis reveals the flexibility of the ADAMTS-5 active site

    Energy Technology Data Exchange (ETDEWEB)

    Shieh, Huey-Sheng; Tomasselli, Alfredo G.; Mathis, Karl J.; Schnute, Mark E.; Woodard, Scott S.; Caspers, Nicole; Williams, Jennifer M.; Kiefer, James R.; Munie, Grace; Wittwer, Arthur; Malfait, Anne-Marie; Tortorella, Micky D. (Pfizer)

    2012-03-02

    A ((1S,2R)-2-hydroxy-2,3-dihydro-1H-inden-1-yl) succinamide derivative (here referred to as Compound 12) shows significant activity toward many matrix metalloproteinases (MMPs), including MMP-2, MMP-8, MMP-9, and MMP-13. Modeling studies had predicted that this compound would not bind to ADAMTS-5 (a disintegrin and metalloproteinase with thrombospondin motifs-5) due to its shallow S1' pocket. However, inhibition analysis revealed it to be a nanomolar inhibitor of both ADAMTS-4 and -5. The observed inconsistency was explained by analysis of crystallographic structures, which showed that Compound 12 in complex with the catalytic domain of ADAMTS-5 (cataTS5) exhibits an unusual conformation in the S1' pocket of the protein. This first demonstration that cataTS5 can undergo an induced conformational change in its active site pocket by a molecule like Compound 12 should enable the design of new aggrecanase inhibitors with better potency and selectivity profiles.

  13. Principal Component Analysis reveals correlation of cavities evolution and functional motions in proteins.

    Science.gov (United States)

    Desdouits, Nathan; Nilges, Michael; Blondel, Arnaud

    2015-02-01

    Protein conformation has been recognized as the key feature determining biological function, as it determines the position of the essential groups specifically interacting with substrates. Hence, the shape of the cavities or grooves at the protein surface appears to drive those functions. However, only a few studies describe the geometrical evolution of protein cavities during molecular dynamics simulations (MD), usually with a crude representation. To unveil the dynamics of cavity geometry evolution, we developed an approach combining cavity detection and Principal Component Analysis (PCA). This approach was applied to four systems subjected to MD (lysozyme, sperm whale myoglobin, Dengue envelope protein and EF-CaM complex). PCA on cavities allows us to perform efficient analysis and classification of the geometry diversity explored by a cavity. Additionally, it reveals correlations between the evolutions of the cavities and structures, and can even suggest how to modify the protein conformation to induce a given cavity geometry. It also helps to perform fast and consensual clustering of conformations according to cavity geometry. Finally, using this approach, we show that both carbon monoxide (CO) location and transfer among the different xenon sites of myoglobin are correlated with few cavity evolution modes of high amplitude. This correlation illustrates the link between ligand diffusion and the dynamic network of internal cavities.

  14. Comparative transcriptome and proteome analysis to reveal the biosynthesis of gold nanoparticles in Arabidopsis.

    Science.gov (United States)

    Tiwari, Manish; Krishnamurthy, Sneha; Shukla, Devesh; Kiiskila, Jeffrey; Jain, Ajay; Datta, Rupali; Sharma, Nilesh; Sahi, Shivendra V

    2016-02-23

    A large number of plants have been tested and exploited in search of a green chemistry approach for the fabrication of gold or other precious metal nanomaterials. Despite the potential of plant based methods, very little is known about the underlying biochemical reactions and genes involved in the biotransformation mechanism of AuCl4 into gold nanoparticles (AuNPs). In this research, we thus focused on studying the effect of Au on growth and nanoparticles formation by analyses of transcriptome, proteome and ionome shift in Arabidopsis. Au exposure favored the growth of Arabidopsis seedling and induced formation of nanoparticles in root and shoot, as indicated by optical and hyperspectral imaging. Root transcriptome analysis demonstrated the differential expression of the members of WRKY, MYB and BHLH gene families, which are involved in the Fe and other essential metals homeostasis. The proteome analysis revealed that Glutathione S-transferases were induced in the shoot and suggested its potential role in the biosynthesis AuNPs. This study also demonstrated the role of plant hormone auxin in determining the Au induced root system architecture. This is the first study using an integrated approach to understand the in planta biotransformation of KAuCl4 into AuNPs.

  15. Metagenomic analysis reveals symbiotic relationship among bacteria in Microcystis-dominated community

    Directory of Open Access Journals (Sweden)

    Meili eXie

    2016-02-01

    Full Text Available Microcystis bloom, a cyanobacterial mass occurrence often found in eutrophicated water bodies, is one of the most serious threats to freshwater ecosystems worldwide. In nature, Microcystis forms aggregates or colonies that contain heterotrophic bacteria. The Microcystis-bacteria colonies were persistent even when they were maintained in lab culture for a long period. The relationship between Microcystis and the associated bacteria was investigated by a metagenomic approach in this study. We developed a visualization-guided method of binning for genome assembly after total colony DNA sequencing. We found that the method was effective in grouping sequences and it did not require reference genome sequence. Individual genomes of the colony bacteria were obtained and they provided valuable insights into microbial community structures. Analysis of metabolic pathways based on these genomes revealed that while all heterotrophic bacteria were dependent upon Microcystis for carbon and energy, Vitamin B12 biosynthesis, which is required for growth by Microcystis, was accomplished in a cooperative fashion among the bacteria. Our analysis also suggests that individual bacteria in the colony community contributed a complete pathway for degradation of benzoate, which is inhibitory to the cyanobacterial growth, and its ecological implication for Microcystis bloom is discussed.

  16. Proteomic analysis of mice fed methionine and choline deficient diet reveals marker proteins associated with steatohepatitis.

    Directory of Open Access Journals (Sweden)

    Su Jin Lee

    Full Text Available The mechanisms underlying the progression of simple steatosis to steatohepatitis are yet to be elucidated. To identify the proteins involved in the development of liver tissue inflammation, we performed comparative proteomic analysis of non-alcoholic steatohepatitis (NASH. Mice fed a methionine and choline deficient diet (MCD developed hepatic steatosis characterized by increased free fatty acid (FFA and triglyceride levels as well as alpha-SMA. Two-dimensional proteomic analysis revealed that the change from the normal diet to the MCD diet affected the expressions of 50 proteins. The most-pronounced changes were observed in the expression of proteins involved in Met metabolism and oxidative stress, most of which were significantly downregulated in NASH model animals. Peroxiredoxin (Prx is the most interesting among the modulated proteins identified in this study. In particular, cross-regulated Prx1 and Prx6 are likely to participate in cellular defense against the development of hepatitis. Thus, these Prx isoforms may be a useful new marker for early stage steatohepatitis. Moreover, curcumin treatment results in alleviation of the severity of hepatic inflammation in steatohepatitis. Notably, curcumin administration in MCD-fed mice dramatically reduced CYP2E1 as well as Prx1 expression, while upregulating Prx6 expression. These findings suggest that curcumin may have a protective role against MCD fed-induced oxidative stress.

  17. Bioinformatic analysis of the neprilysin (M13 family of peptidases reveals complex evolutionary and functional relationships

    Directory of Open Access Journals (Sweden)

    Pinney John W

    2008-01-01

    Full Text Available Abstract Background The neprilysin (M13 family of endopeptidases are zinc-metalloenzymes, the majority of which are type II integral membrane proteins. The best characterised of this family is neprilysin, which has important roles in inactivating signalling peptides involved in modulating neuronal activity, blood pressure and the immune system. Other family members include the endothelin converting enzymes (ECE-1 and ECE-2, which are responsible for the final step in the synthesis of potent vasoconstrictor endothelins. The ECEs, as well as neprilysin, are considered valuable therapeutic targets for treating cardiovascular disease. Other members of the M13 family have not been functionally characterised, but are also likely to have biological roles regulating peptide signalling. The recent sequencing of animal genomes has greatly increased the number of M13 family members in protein databases, information which can be used to reveal evolutionary relationships and to gain insight into conserved biological roles. Results The phylogenetic analysis successfully resolved vertebrate M13 peptidases into seven classes, one of which appears to be specific to mammals, and insect genes into five functional classes and a series of expansions, which may include inactive peptidases. Nematode genes primarily resolved into groups containing no other taxa, bar the two nematode genes associated with Drosophila DmeNEP1 and DmeNEP4. This analysis reconstructed only one relationship between chordate and invertebrate clusters, that of the ECE sub-group and the DmeNEP3 related genes. Analysis of amino acid utilisation in the active site of M13 peptidases reveals a basis for their biochemical properties. A relatively invariant S1' subsite gives the majority of M13 peptidases their strong preference for hydrophobic residues in P1' position. The greater variation in the S2' subsite may be instrumental in determining the specificity of M13 peptidases for their substrates

  18. Converging Information and Communication Systems

    DEFF Research Database (Denmark)

    Øst, Alexander

    2003-01-01

    This Ph.D.-project investigates the process of convergence between television and computers. Identifying three “promises of convergence”: interoperability, quality and interactivity, the project examines the key properties of the computer and television sectors, which have had - or are expected...... in the future to have - significant importance to the process and consequences of the convergence. The project focuses on the appliances, i.e. the TV sets, the computers and their peripheral equipment. It also takes into account the infrastructure and signals, which contain and deliver the information...... and communication, as well as the nature of the content and the usage scenarios of consumers....

  19. Verification of the coupled fluid/solid transfer in a CASL grid-to-rod-fretting simulation : a technical brief on the analysis of convergence behavior and demonstration of software tools for verification.

    Energy Technology Data Exchange (ETDEWEB)

    Copps, Kevin D.

    2011-12-01

    For a CASL grid-to-rod fretting problem, Sandia's Percept software was used in conjunction with the Sierra Mechanics suite to analyze the convergence behavior of the data transfer from a fluid simulation to a solid mechanics simulation. An analytic function, with properties relatively close to numerically computed fluid approximations, was chosen to represent the pressure solution in the fluid domain. The analytic pressure was interpolated on a sequence of grids on the fluid domain, and transferred onto a separate sequence of grids in the solid domain. The error in the resulting pressure in the solid domain was measured with respect to the analytic pressure. The error in pressure approached zero as both the fluid and solids meshes were refined. The convergence of the transfer algorithm was limited by whether the source grid resolution was the same or finer than the target grid resolution. In addition, using a feature coverage analysis, we found gaps in the solid mechanics code verification test suite directly relevant to the prototype CASL GTRF simulations.

  20. Time series analysis of satellite data reveals continuous deforestation of New England since the 1980s

    Science.gov (United States)

    Olofsson, Pontus; Holden, Christopher E.; Bullock, Eric L.; Woodcock, Curtis E.

    2016-06-01

    Land cover and land change were monitored continuously between 1985 and 2011 at 30 m resolution across New England in the Northeastern United States in support of modeling the terrestrial carbon budget. It was found that the forest area has been decreasing throughout the study period in each state of the region since the 1980s. A total of 386 657 ± 98 137 ha (95% confidence interval) of forest has been converted to other land covers since 1985. Mainly driven by low density residential development, the deforestation accelerated in the mid-1990s until 2007 when it plateaued as a result of declining new residential construction and in turn, the financial crisis of 2007-08. The area of forest harvest, estimated at 226 519 ± 66 682 ha, was mapped separately and excluded from the deforestation estimate, while the area of forest expansion on non-forested lands was found to not be significantly different from zero. New England is often held as a principal example of a forest transition with historical widespread deforestation followed by recovery of forestlands as farming activities diminished, but the results of this study support the notion of a reversal of the forest transition as the region again is experiencing widespread deforestation. All available Landsat imagery acquired after 1985 for the study area were collected and used in the analysis. Areas of land cover and land change were estimated from a random sample of reference observations stratified by a twelve-class land change map encompassing the entire study area and period. The statistical analysis revealed that the net change in forest area and the associated modeled impact on the terrestrial carbon balance would have been considerably different if the results of the map were used without inferring the area of forest change by analysis of a reference sample.

  1. Transcriptional regulation of rod photoreceptor homeostasis revealed by in vivo NRL targetome analysis.

    Directory of Open Access Journals (Sweden)

    Hong Hao

    Full Text Available A stringent control of homeostasis is critical for functional maintenance and survival of neurons. In the mammalian retina, the basic motif leucine zipper transcription factor NRL determines rod versus cone photoreceptor cell fate and activates the expression of many rod-specific genes. Here, we report an integrated analysis of NRL-centered gene regulatory network by coupling chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-Seq data from Illumina and ABI platforms with global expression profiling and in vivo knockdown studies. We identified approximately 300 direct NRL target genes. Of these, 22 NRL targets are associated with human retinal dystrophies, whereas 95 mapped to regions of as yet uncloned retinal disease loci. In silico analysis of NRL ChIP-Seq peak sequences revealed an enrichment of distinct sets of transcription factor binding sites. Specifically, we discovered that genes involved in photoreceptor function include binding sites for both NRL and homeodomain protein CRX. Evaluation of 26 ChIP-Seq regions validated their enhancer functions in reporter assays. In vivo knockdown of 16 NRL target genes resulted in death or abnormal morphology of rod photoreceptors, suggesting their importance in maintaining retinal function. We also identified histone demethylase Kdm5b as a novel secondary node in NRL transcriptional hierarchy. Exon array analysis of flow-sorted photoreceptors in which Kdm5b was knocked down by shRNA indicated its role in regulating rod-expressed genes. Our studies identify candidate genes for retinal dystrophies, define cis-regulatory module(s for photoreceptor-expressed genes and provide a framework for decoding transcriptional regulatory networks that dictate rod homeostasis.

  2. ALE meta-analysis reveals dissociable networks for affective and discriminative aspects of touch.

    Science.gov (United States)

    Morrison, India

    2016-04-01

    Emotionally-laden tactile stimulation-such as a caress on the skin or the feel of velvet-may represent a functionally distinct domain of touch, underpinned by specific cortical pathways. In order to determine whether, and to what extent, cortical functional neuroanatomy supports a distinction between affective and discriminative touch, an activation likelihood estimate (ALE) meta-analysis was performed. This meta-analysis statistically mapped reported functional magnetic resonance imaging (fMRI) activations from 17 published affective touch studies in which tactile stimulation was associated with positive subjective evaluation (n = 291, 34 experimental contrasts). A separate ALE meta-analysis mapped regions most likely to be activated by tactile stimulation during detection and discrimination tasks (n = 1,075, 91 experimental contrasts). These meta-analyses revealed dissociable regions for affective and discriminative touch, with posterior insula (PI) more likely to be activated for affective touch, and primary somatosensory cortices (SI) more likely to be activated for discriminative touch. Secondary somatosensory cortex had a high likelihood of engagement by both affective and discriminative touch. Further, meta-analytic connectivity (MCAM) analyses investigated network-level co-activation likelihoods independent of task or stimulus, across a range of domains and paradigms. Affective-related PI and discriminative-related SI regions co-activated with different networks, implicated in dissociable functions, but sharing somatosensory co-activations. Taken together, these meta-analytic findings suggest that affective and discriminative touch are dissociable both on the regional and network levels. However, their degree of shared activation likelihood in somatosensory cortices indicates that this dissociation reflects functional biases within tactile processing networks, rather than functionally and anatomically distinct pathways.

  3. Genome-wide analysis of gene expression in primate taste buds reveals links to diverse processes.

    Directory of Open Access Journals (Sweden)

    Peter Hevezi

    Full Text Available Efforts to unravel the mechanisms underlying taste sensation (gustation have largely focused on rodents. Here we present the first comprehensive characterization of gene expression in primate taste buds. Our findings reveal unique new insights into the biology of taste buds. We generated a taste bud gene expression database using laser capture microdissection (LCM procured fungiform (FG and circumvallate (CV taste buds from primates. We also used LCM to collect the top and bottom portions of CV taste buds. Affymetrix genome wide arrays were used to analyze gene expression in all samples. Known taste receptors are preferentially expressed in the top portion of taste buds. Genes associated with the cell cycle and stem cells are preferentially expressed in the bottom portion of taste buds, suggesting that precursor cells are located there. Several chemokines including CXCL14 and CXCL8 are among the highest expressed genes in taste buds, indicating that immune system related processes are active in taste buds. Several genes expressed specifically in endocrine glands including growth hormone releasing hormone and its receptor are also strongly expressed in taste buds, suggesting a link between metabolism and taste. Cell type-specific expression of transcription factors and signaling molecules involved in cell fate, including KIT, reveals the taste bud as an active site of cell regeneration, differentiation, and development. IKBKAP, a gene mutated in familial dysautonomia, a disease that results in loss of taste buds, is expressed in taste cells that communicate with afferent nerve fibers via synaptic transmission. This database highlights the power of LCM coupled with transcriptional profiling to dissect the molecular composition of normal tissues, represents the most comprehensive molecular analysis of primate taste buds to date, and provides a foundation for further studies in diverse aspects of taste biology.

  4. Analysis of virus genomes from glacial environments reveals novel virus groups with unusual host interactions

    Science.gov (United States)

    Bellas, Christopher M.; Anesio, Alexandre M.; Barker, Gary

    2015-01-01

    Microbial communities in glacial ecosystems are diverse, active, and subjected to strong viral pressures and infection rates. In this study we analyse putative virus genomes assembled from three dsDNA viromes from cryoconite hole ecosystems of Svalbard and the Greenland Ice Sheet to assess the potential hosts and functional role viruses play in these habitats. We assembled 208 million reads from the virus-size fraction and developed a procedure to select genuine virus scaffolds from cellular contamination. Our curated virus library contained 546 scaffolds up to 230 Kb in length, 54 of which were circular virus consensus genomes. Analysis of virus marker genes revealed a wide range of viruses had been assembled, including bacteriophages, cyanophages, nucleocytoplasmic large DNA viruses and a virophage, with putative hosts identified as Cyanobacteria, Alphaproteobacteria, Gammaproteobacteria, Actinobacteria, Firmicutes, eukaryotic algae and amoebae. Whole genome comparisons revealed the majority of circular genome scaffolds (CGS) formed 12 novel groups, two of which contained multiple phage members with plasmid-like properties, including a group of phage-plasmids possessing plasmid-like partition genes and toxin-antitoxin addiction modules to ensure their replication and a satellite phage-plasmid group. Surprisingly we also assembled a phage that not only encoded plasmid partition genes, but a clustered regularly interspaced short palindromic repeat (CRISPR)/Cas adaptive bacterial immune system. One of the spacers was an exact match for another phage in our virome, indicating that in a novel use of the system, the lysogen was potentially capable of conferring immunity on its bacterial host against other phage. Together these results suggest that highly novel and diverse groups of viruses are present in glacial environments, some of which utilize very unusual life strategies and genes to control their replication and maintain a long-term relationship with their hosts

  5. Comparative whole-genome analysis of clinical isolates reveals characteristic architecture of Mycobacterium tuberculosis pangenome.

    Science.gov (United States)

    Periwal, Vinita; Patowary, Ashok; Vellarikkal, Shamsudheen Karuthedath; Gupta, Anju; Singh, Meghna; Mittal, Ashish; Jeyapaul, Shamini; Chauhan, Rajendra Kumar; Singh, Ajay Vir; Singh, Pravin Kumar; Garg, Parul; Katoch, Viswa Mohan; Katoch, Kiran; Chauhan, Devendra Singh; Sivasubbu, Sridhar; Scaria, Vinod

    2015-01-01

    The tubercle complex consists of closely related mycobacterium species which appear to be variants of a single species. Comparative genome analysis of different strains could provide useful clues and insights into the genetic diversity of the species. We integrated genome assemblies of 96 strains from Mycobacterium tuberculosis complex (MTBC), which included 8 Indian clinical isolates sequenced and assembled in this study, to understand its pangenome architecture. We predicted genes for all the 96 strains and clustered their respective CDSs into homologous gene clusters (HGCs) to reveal a hard-core, soft-core and accessory genome component of MTBC. The hard-core (HGCs shared amongst 100% of the strains) was comprised of 2,066 gene clusters whereas the soft-core (HGCs shared amongst at least 95% of the strains) comprised of 3,374 gene clusters. The change in the core and accessory genome components when observed as a function of their size revealed that MTBC has an open pangenome. We identified 74 HGCs that were absent from reference strains H37Rv and H37Ra but were present in most of clinical isolates. We report PCR validation on 9 candidate genes depicting 7 genes completely absent from H37Rv and H37Ra whereas 2 genes shared partial homology with them accounting to probable insertion and deletion events. The pangenome approach is a promising tool for studying strain specific genetic differences occurring within species. We also suggest that since selecting appropriate target genes for typing purposes requires the expected target gene be present in all isolates being typed, therefore estimating the core-component of the species becomes a subject of prime importance.

  6. Comparative whole-genome analysis of clinical isolates reveals characteristic architecture of Mycobacterium tuberculosis pangenome.

    Directory of Open Access Journals (Sweden)

    Vinita Periwal

    Full Text Available The tubercle complex consists of closely related mycobacterium species which appear to be variants of a single species. Comparative genome analysis of different strains could provide useful clues and insights into the genetic diversity of the species. We integrated genome assemblies of 96 strains from Mycobacterium tuberculosis complex (MTBC, which included 8 Indian clinical isolates sequenced and assembled in this study, to understand its pangenome architecture. We predicted genes for all the 96 strains and clustered their respective CDSs into homologous gene clusters (HGCs to reveal a hard-core, soft-core and accessory genome component of MTBC. The hard-core (HGCs shared amongst 100% of the strains was comprised of 2,066 gene clusters whereas the soft-core (HGCs shared amongst at least 95% of the strains comprised of 3,374 gene clusters. The change in the core and accessory genome components when observed as a function of their size revealed that MTBC has an open pangenome. We identified 74 HGCs that were absent from reference strains H37Rv and H37Ra but were present in most of clinical isolates. We report PCR validation on 9 candidate genes depicting 7 genes completely absent from H37Rv and H37Ra whereas 2 genes shared partial homology with them accounting to probable insertion and deletion events. The pangenome approach is a promising tool for studying strain specific genetic differences occurring within species. We also suggest that since selecting appropriate target genes for typing purposes requires the expected target gene be present in all isolates being typed, therefore estimating the core-component of the species becomes a subject of prime importance.

  7. Analysis of SSH library of rice variety Aganni reveals candidate gall midge resistance genes.

    Science.gov (United States)

    Divya, Dhanasekar; Singh, Y Tunginba; Nair, Suresh; Bentur, J S

    2016-03-01

    The Asian rice gall midge, Orseolia oryzae, is a serious insect pest causing extensive yield loss. Interaction between the gall midge and rice genotypes is known to be on a gene-for-gene basis. Here, we report molecular basis of HR- (hypersensitive reaction-negative) type of resistance in Aganni (an indica rice variety possessing gall midge resistance gene Gm8) through the construction and analysis of a suppressive subtraction hybridization (SSH) cDNA library. In all, 2,800 positive clones were sequenced and analyzed. The high-quality ESTs were assembled into 448 non-redundant gene sequences. Homology search with the NCBI databases, using BlastX and BlastN, revealed that 73% of the clones showed homology to genes with known function and majority of ESTs belonged to the gene ontology category 'biological process'. Validation of 27 putative candidate gall midge resistance genes through real-time PCR, following gall midge infestation, in contrasting parents and their derived pre-NILs (near isogenic lines) revealed induction of specific genes related to defense and metabolism. Interestingly, four genes, belonging to families of leucine-rich repeat (LRR), heat shock protein (HSP), pathogenesis related protein (PR), and NAC domain-containing protein, implicated in conferring HR+ type of resistance, were found to be up-regulated in Aganni. Two of the reactive oxygen intermediates (ROI)-scavenging-enzyme-coding genes Cytosolic Ascorbate Peroxidase1, 2 (OsAPx1 and OsAPx2) were found up-regulated in Aganni in incompatible interaction possibly suppressing HR. We suggest that Aganni has a deviant form of inducible, salicylic acid (SA)-mediated resistance but without HR.

  8. Systematic prioritization and integrative analysis of copy number variations in schizophrenia reveal key schizophrenia susceptibility genes.

    Science.gov (United States)

    Luo, Xiongjian; Huang, Liang; Han, Leng; Luo, Zhenwu; Hu, Fang; Tieu, Roger; Gan, Lin

    2014-11-01

    Schizophrenia is a common mental disorder with high heritability and strong genetic heterogeneity. Common disease-common variants hypothesis predicts that schizophrenia is attributable in part to common genetic variants. However, recent studies have clearly demonstrated that copy number variations (CNVs) also play pivotal roles in schizophrenia susceptibility and explain a proportion of missing heritability. Though numerous CNVs have been identified, many of the regions affected by CNVs show poor overlapping among different studies, and it is not known whether the genes disrupted by CNVs contribute to the risk of schizophrenia. By using cumulative scoring, we systematically prioritized the genes affected by CNVs in schizophrenia. We identified 8 top genes that are frequently disrupted by CNVs, including NRXN1, CHRNA7, BCL9, CYFIP1, GJA8, NDE1, SNAP29, and GJA5. Integration of genes affected by CNVs with known schizophrenia susceptibility genes (from previous genetic linkage and association studies) reveals that many genes disrupted by CNVs are also associated with schizophrenia. Further protein-protein interaction (PPI) analysis indicates that protein products of genes affected by CNVs frequently interact with known schizophrenia-associated proteins. Finally, systematic integration of CNVs prioritization data with genetic association and PPI data identifies key schizophrenia candidate genes. Our results provide a global overview of genes impacted by CNVs in schizophrenia and reveal a densely interconnected molecular network of de novo CNVs in schizophrenia. Though the prioritized top genes represent promising schizophrenia risk genes, further work with different prioritization methods and independent samples is needed to confirm these findings. Nevertheless, the identified key candidate genes may have important roles in the pathogenesis of schizophrenia, and further functional characterization of these genes may provide pivotal targets for future therapeutics and

  9. Dysconnection topography in schizophrenia revealed with state-space analysis of EEG.

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    Mahdi Jalili

    Full Text Available BACKGROUND: The dysconnection hypothesis has been proposed to account for pathophysiological mechanisms underlying schizophrenia. Widespread structural changes suggesting abnormal connectivity in schizophrenia have been imaged. A functional counterpart of the structural maps would be the EEG synchronization maps. However, due to the limits of currently used bivariate methods, functional correlates of dysconnection are limited to the isolated measurements of synchronization between preselected pairs of EEG signals. METHODS/RESULTS: To reveal a whole-head synchronization topography in schizophrenia, we applied a new method of multivariate synchronization analysis called S-estimator to the resting dense-array (128 channels EEG obtained from 14 patients and 14 controls. This method determines synchronization from the embedding dimension in a state-space domain based on the theoretical consequence of the cooperative behavior of simultaneous time series-the shrinking of the state-space embedding dimension. The S-estimator imaging revealed a specific synchronization landscape in schizophrenia patients. Its main features included bilaterally increased synchronization over temporal brain regions and decreased synchronization over the postcentral/parietal region neighboring the midline. The synchronization topography was stable over the course of several months and correlated with the severity of schizophrenia symptoms. In particular, direct correlations linked positive, negative, and general psychopathological symptoms to the hyper-synchronized temporal clusters over both hemispheres. Along with these correlations, general psychopathological symptoms inversely correlated within the hypo-synchronized postcentral midline region. While being similar to the structural maps of cortical changes in schizophrenia, the S-maps go beyond the topography limits, demonstrating a novel aspect of the abnormalities of functional cooperation: namely, regionally reduced or

  10. Privatization, convergence, and institutional autonomy

    NARCIS (Netherlands)

    Rooijen, van M.

    2011-01-01

    Some of the trends incoming for 2011 – greater institutional autonomy, public/private convergence, entrepreneurial management, civic engagement – suggest innovation for hard times, with socio-economic and political rationales increasingly driving borderless developments. Others – open learning and h

  11. Financial Development and Convergence Clubs

    NARCIS (Netherlands)

    N. Basturk (Nalan); R. Paap (Richard); D.J.C. van Dijk (Dick)

    2010-01-01

    textabstractThis paper studies the economic development process, measured by Gross Domestic Product (GDP), for a large panel of countries. We propose a methodology that identifies groups of countries (convergence clubs) that show similar GDP structures, while allowing for changes in club membersh

  12. Industrial Evolution Through Complementary Convergence

    DEFF Research Database (Denmark)

    Frøslev Christensen, Jens

    2011-01-01

    The article addresses the dynamics through which product markets become derailed from early product life cycle (PLC)-tracks and engaged in complementary convergence with other product markets or industries. We compare and contrast the theories that can explain, respectively, the PLC...

  13. Convergence of a Catalan Series

    Science.gov (United States)

    Koshy, Thomas; Gao, Zhenguang

    2012-01-01

    This article studies the convergence of the infinite series of the reciprocals of the Catalan numbers. We extract the sum of the series as well as some related ones, illustrating the power of the calculus in the study of the Catalan numbers.

  14. Digital Convergence and Content Regulation

    NARCIS (Netherlands)

    Starks, Michael John

    2014-01-01

    abstractBroadcasting, Press and Internet journalism systems of distribution are converging: the same infrastructure can deliver all three historically separate services. Reception devices mirror this: the Connected TV, the tablet and the smart phone overlap in their functionality. Service overlaps a

  15. Exponential convergence rate in entropy

    Institute of Scientific and Technical Information of China (English)

    Mu-Fa Chen

    2007-01-01

    The exponential convergence rate in entropy is studied for symmetric forms, with a specia! attention to the Markov chain with a state space having two points only. Some upper and lower bounds of the rate are obtained and five examples with precise or qualitatively exact estimates are presented.

  16. Converge, Version 3.0

    Directory of Open Access Journals (Sweden)

    Lotfi Tadj

    1993-01-01

    Full Text Available Although intended for college teachers/students, Converge presents a feature that may interest all scientists: it allows an easy export of graphic files to most known word processors, specifically to the ℙ, Version 2.1, a powerful WYSIWYG mathematical word processor.

  17. A proof of convergence of the concave-convex procedure using Zangwill's theory.

    Science.gov (United States)

    Sriperumbudur, Bharath K; Lanckriet, Gert R G

    2012-06-01

    The concave-convex procedure (CCCP) is an iterative algorithm that solves d.c. (difference of convex functions) programs as a sequence of convex programs. In machine learning, CCCP is extensively used in many learning algorithms, including sparse support vector machines (SVMs), transductive SVMs, and sparse principal component analysis. Though CCCP is widely used in many applications, its convergence behavior has not gotten a lot of specific attention. Yuille and Rangarajan analyzed its convergence in their original paper; however, we believe the analysis is not complete. The convergence of CCCP can be derived from the convergence of the d.c. algorithm (DCA), proposed in the global optimization literature to solve general d.c. programs, whose proof relies on d.c. duality. In this note, we follow a different reasoning and show how Zangwill's global convergence theory of iterative algorithms provides a natural framework to prove the convergence of CCCP. This underlines Zangwill's theory as a powerful and general framework to deal with the convergence issues of iterative algorithms, after also being used to prove the convergence of algorithms like expectation-maximization and generalized alternating minimization. In this note, we provide a rigorous analysis of the convergence of CCCP by addressing two questions: When does CCCP find a local minimum or a stationary point of the d.c. program under consideration? and when does the sequence generated by CCCP converge? We also present an open problem on the issue of local convergence of CCCP.

  18. Comparative transcriptome analysis reveals the genetic basis of skin color variation in common carp.

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    Yanliang Jiang

    Full Text Available The common carp is an important aquaculture species that is widely distributed across the world. During the long history of carp domestication, numerous carp strains with diverse skin colors have been established. Skin color is used as a visual criterion to determine the market value of carp. However, the genetic basis of common carp skin color has not been extensively studied.In this study, we performed Illumina sequencing on two common carp strains: the reddish Xingguo red carp and the brownish-black Yellow River carp. A total of 435,348,868 reads were generated, resulting in 198,781 assembled contigs that were used as reference sequences. Comparisons of skin transcriptome files revealed 2,012 unigenes with significantly different expression in the two common carp strains, including 874 genes that were up-regulated in Xingguo red carp and 1,138 genes that were up-regulated in Yellow River carp. The expression patterns of 20 randomly selected differentially expressed genes were validated using quantitative RT-PCR. Gene pathway analysis of the differentially expressed genes indicated that melanin biosynthesis, along with the Wnt and MAPK signaling pathways, is highly likely to affect the skin pigmentation process. Several key genes involved in the skin pigmentation process, including TYRP1, SILV, ASIP and xCT, showed significant differences in their expression patterns between the two strains.In this study, we conducted a comparative transcriptome analysis of Xingguo red carp and Yellow River carp skins, and we detected key genes involved in the common carp skin pigmentation process. We propose that common carp skin pigmentation depends upon at least three pathways. Understanding fish skin color genetics will facilitate future molecular selection of the fish skin colors with high market values.

  19. Comparative Transcriptome Analysis Reveals the Genetic Basis of Skin Color Variation in Common Carp

    Science.gov (United States)

    Jiang, Yanliang; Zhang, Songhao; Xu, Jian; Feng, Jianxin; Mahboob, Shahid; Al-Ghanim, Khalid A.; Sun, Xiaowen; Xu, Peng

    2014-01-01

    Background The common carp is an important aquaculture species that is widely distributed across the world. During the long history of carp domestication, numerous carp strains with diverse skin colors have been established. Skin color is used as a visual criterion to determine the market value of carp. However, the genetic basis of common carp skin color has not been extensively studied. Methodology/Principal Findings In this study, we performed Illumina sequencing on two common carp strains: the reddish Xingguo red carp and the brownish-black Yellow River carp. A total of 435,348,868 reads were generated, resulting in 198,781 assembled contigs that were used as reference sequences. Comparisons of skin transcriptome files revealed 2,012 unigenes with significantly different expression in the two common carp strains, including 874 genes that were up-regulated in Xingguo red carp and 1,138 genes that were up-regulated in Yellow River carp. The expression patterns of 20 randomly selected differentially expressed genes were validated using quantitative RT-PCR. Gene pathway analysis of the differentially expressed genes indicated that melanin biosynthesis, along with the Wnt and MAPK signaling pathways, is highly likely to affect the skin pigmentation process. Several key genes involved in the skin pigmentation process, including TYRP1, SILV, ASIP and xCT, showed significant differences in their expression patterns between the two strains. Conclusions In this study, we conducted a comparative transcriptome analysis of Xingguo red carp and Yellow River carp skins, and we detected key genes involved in the common carp skin pigmentation process. We propose that common carp skin pigmentation depends upon at least three pathways. Understanding fish skin color genetics will facilitate future molecular selection of the fish skin colors with high market values. PMID:25255374

  20. Analysis of the full genome of human group C rotaviruses reveals lineage diversification and reassortment.

    Science.gov (United States)

    Medici, Maria Cristina; Tummolo, Fabio; Martella, Vito; Arcangeletti, Maria Cristina; De Conto, Flora; Chezzi, Carlo; Fehér, Enikő; Marton, Szilvia; Calderaro, Adriana; Bányai, Krisztián

    2016-08-01

    Group C rotaviruses (RVC) are enteric pathogens of humans and animals. Whole-genome sequences are available only for few RVCs, leaving gaps in our knowledge about their genetic diversity. We determined the full-length genome sequence of two human RVCs (PR2593/2004 and PR713/2012), detected in Italy from hospital-based surveillance for rotavirus infection in 2004 and 2012. In the 11 RNA genomic segments, the two Italian RVCs segregated within separate intra-genotypic lineages showed variation ranging from 1.9 % (VP6) to 15.9 % (VP3) at the nucleotide level. Comprehensive analysis of human RVC sequences available in the databases allowed us to reveal the existence of at least two major genome configurations, defined as type I and type II. Human RVCs of type I were all associated with the M3 VP3 genotype, including the Italian strain PR2593/2004. Conversely, human RVCs of type II were all associated with the M2 VP3 genotype, including the Italian strain PR713/2012. Reassortant RVC strains between these major genome configurations were identified. Although only a few full-genome sequences of human RVCs, mostly of Asian origin, are available, the analysis of human RVC sequences retrieved from the databases indicates that at least two intra-genotypic RVC lineages circulate in European countries. Gathering more sequence data is necessary to develop a standardized genotype and intra-genotypic lineage classification system useful for epidemiological investigations and avoiding confusion in the literature.

  1. Comparative Genomic Analysis of Clinical and Environmental Vibrio Vulnificus Isolates Revealed Biotype 3 Evolutionary Relationships

    Directory of Open Access Journals (Sweden)

    Yael eKotton

    2015-01-01

    Full Text Available In 1996 a common-source outbreak of severe soft tissue and bloodstream infections erupted among Israeli fish farmers and fish consumers due to changes in fish marketing policies. The causative pathogen was a new strain of Vibrio vulnificus, named biotype 3, which displayed a unique biochemical and genotypic profile. Initial observations suggested that the pathogen erupted as a result of genetic recombination between two distinct populations. We applied a whole genome shotgun sequencing approach using several V. vulnificus strains from Israel in order to study the pan genome of V. vulnificus and determine the phylogenetic relationship of biotype 3 with existing populations. The core genome of V. vulnificus based on 16 draft and complete genomes consisted of 3068 genes, representing between 59% and 78% of the whole genome of 16 strains. The accessory genome varied in size from 781 kbp to 2044 kbp. Phylogenetic analysis based on whole, core, and accessory genomes displayed similar clustering patterns with two main clusters, clinical (C and environmental (E, all biotype 3 strains formed a distinct group within the E cluster. Annotation of accessory genomic regions found in biotype 3 strains and absent from the core genome yielded 1732 genes, of which the vast majority encoded hypothetical proteins, phage-related proteins, and mobile element proteins. A total of 1916 proteins (including 713 hypothetical proteins were present in all human pathogenic strains (both biotype 3 and non-biotype 3 and absent from the environmental strains. Clustering analysis of the non-hypothetical proteins revealed 148 protein clusters shared by all human pathogenic strains; these included transcriptional regulators, arylsulfatases, methyl-accepting chemotaxis proteins, acetyltransferases, GGDEF family proteins, transposases, type IV secretory system (T4SS proteins, and integrases. Our study showed that V. vulnificus biotype 3 evolved from environmental populations and

  2. Comorbid Analysis of Genes Associated with Autism Spectrum Disorders Reveals Differential Evolutionary Constraints

    Science.gov (United States)

    David, Maude M.; Enard, David; Ozturk, Alp; Daniels, Jena; Jung, Jae-Yoon; Diaz-Beltran, Leticia; Wall, Dennis. P.

    2016-01-01

    The burden of comorbidity in Autism Spectrum Disorder (ASD) is substantial. The symptoms of autism overlap with many other human conditions, reflecting common molecular pathologies suggesting that cross-disorder analysis will help prioritize autism gene candidates. Genes in the intersection between autism and related conditions may represent nonspecific indicators of dysregulation while genes unique to autism may play a more causal role. Thorough literature review allowed us to extract 125 ICD-9 codes comorbid to ASD that we mapped to 30 specific human disorders. In the present work, we performed an automated extraction of genes associated with ASD and its comorbid disorders, and found 1031 genes involved in ASD, among which 262 are involved in ASD only, with the remaining 779 involved in ASD and at least one comorbid disorder. A pathway analysis revealed 13 pathways not involved in any other comorbid disorders and therefore unique to ASD, all associated with basal cellular functions. These pathways differ from the pathways associated with both ASD and its comorbid conditions, with the latter being more specific to neural function. To determine whether the sequence of these genes have been subjected to differential evolutionary constraints, we studied long term constraints by looking into Genomic Evolutionary Rate Profiling, and showed that genes involved in several comorbid disorders seem to have undergone more purifying selection than the genes involved in ASD only. This result was corroborated by a higher dN/dS ratio for genes unique to ASD as compare to those that are shared between ASD and its comorbid disorders. Short-term evolutionary constraints showed the same trend as the pN/pS ratio indicates that genes unique to ASD were under significantly less evolutionary constraint than the genes associated with all other disorders. PMID:27414027

  3. Secretome analysis of Aspergillus fumigatus reveals Asp-hemolysin as a major secreted protein.

    Science.gov (United States)

    Wartenberg, Dirk; Lapp, Katrin; Jacobsen, Ilse D; Dahse, Hans-Martin; Kniemeyer, Olaf; Heinekamp, Thorsten; Brakhage, Axel A

    2011-11-01

    Surface-associated and secreted proteins represent primarily exposed components of Aspergillus fumigatus during host infection. Several secreted proteins are known to be involved in defense mechanisms or immune evasion, thus, probably contributing to pathogenicity. Furthermore, several secreted antigens were identified as possible biomarkers for the verification of diseases caused by Aspergillus species. Nevertheless, there is only limited knowledge about the composition of the secretome and about molecular functions of particular proteins. To identify secreted proteins potentially essential for virulence, the core secretome of A. fumigatus grown in minimal medium was determined. Two-dimensional gel electrophoretic separation and subsequent MALDI-TOF-MS/MS analyses resulted in the identification of 64 different proteins. Additionally, secretome analyses of A. fumigatus utilizing elastin, collagen or keratin as main carbon and nitrogen source were performed. Thereby, the alkaline serine protease Alp1 was identified as the most abundant protein and hence presumably represents an important protease during host infection. Interestingly, the Asp-hemolysin (Asp-HS), which belongs to the protein family of aegerolysins and which was often suggested to be involved in fungal virulence, was present in the secretome under all growth conditions tested. In addition, a second, non-secreted protein with an aegerolysin domain annotated as Asp-hemolysin-like (HS-like) protein can be found to be encoded in the genome of A. fumigatus. Generation and analysis of Asp-HS and HS-like deletion strains revealed no differences in phenotype compared to the corresponding wild-type strain. Furthermore, hemolysis and cytotoxicity was not altered in both single-deletion and double-deletion mutants lacking both aegerolysin genes. All mutant strains showed no attenuation in virulence in a mouse infection model for invasive pulmonary aspergillosis. Overall, this study provides a comprehensive

  4. Analysis of gene expression during parabolic flights reveals distinct early gravity responses in Arabidopsis roots.

    Science.gov (United States)

    Aubry-Hivet, D; Nziengui, H; Rapp, K; Oliveira, O; Paponov, I A; Li, Y; Hauslage, J; Vagt, N; Braun, M; Ditengou, F A; Dovzhenko, A; Palme, K

    2014-01-01

    Plant roots are among most intensively studied biological systems in gravity research. Altered gravity induces asymmetric cell growth leading to root bending. Differential distribution of the phytohormone auxin underlies root responses to gravity, being coordinated by auxin efflux transporters from the PIN family. The objective of this study was to compare early transcriptomic changes in roots of Arabidopsis thaliana wild type, and pin2 and pin3 mutants under parabolic flight conditions and to correlate these changes to auxin distribution. Parabolic flights allow comparison of transient 1-g, hypergravity and microgravity effects in living organisms in parallel. We found common and mutation-related genes differentially expressed in response to transient microgravity phases. Gene ontology analysis of common genes revealed lipid metabolism, response to stress factors and light categories as primarily involved in response to transient microgravity phases, suggesting that fundamental reorganisation of metabolic pathways functions upstream of a further signal mediating hormonal network. Gene expression changes in roots lacking the columella-located PIN3 were stronger than in those deprived of the epidermis and cortex cell-specific PIN2. Moreover, repetitive exposure to microgravity/hypergravity and gravity/hypergravity flight phases induced an up-regulation of auxin responsive genes in wild type and pin2 roots, but not in pin3 roots, suggesting a critical function of PIN3 in mediating auxin fluxes in response to transient microgravity phases. Our study provides important insights towards understanding signal transduction processes in transient microgravity conditions by combining for the first time the parabolic flight platform with the transcriptome analysis of different genetic mutants in the model plant, Arabidopsis.

  5. RNA-Seq analysis reveals a six-gene SoxR regulon in Streptomyces coelicolor.

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    Nawar Naseer

    Full Text Available The redox-regulated transcription factor SoxR is conserved in diverse bacteria, but emerging studies suggest that this protein plays distinct physiological roles in different bacteria. SoxR regulates a global oxidative stress response (involving > 100 genes against exogenous redox-cycling drugs in Escherichia coli and related enterics. In the antibiotic producers Streptomyces coelicolor and Pseudomonas aeruginosa, however, SoxR regulates a smaller number of genes that encode membrane transporters and proteins with homology to antibiotic-tailoring enzymes. In both S. coelicolor and P. aeruginosa, SoxR-regulated genes are expressed in stationary phase during the production of endogenously-produced redox-active antibiotics. These observations suggest that SoxR evolved to sense endogenous secondary metabolites and activate machinery to process and transport them in antibiotic-producing bacteria. Previous bioinformatics analysis that searched the genome for SoxR-binding sites in putative promoters defined a five-gene SoxR regulon in S. coelicolor including an ABC transporter, two oxidoreductases, a monooxygenase and an epimerase/dehydratase. Since this in silico screen may have missed potential SoxR-targets, we conducted a whole genome transcriptome comparison of wild type S. coelicolor and a soxR-deficient mutant in stationary phase using RNA-Seq. Our analysis revealed a sixth SoxR-regulated gene in S. coelicolor that encodes a putative quinone oxidoreductase. Knowledge of the full complement of genes regulated by SoxR will facilitate studies to elucidate the function of this regulatory molecule in antibiotic producers.

  6. Proteomic analysis of Ketogulonicigenium vulgare under glutathione reveals high demand for thiamin transport and antioxidant protection.

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    Qian Ma

    Full Text Available Ketogulonicigenium vulgare, though grows poorly when mono-cultured, has been widely used in the industrial production of the precursor of vitamin C with the coculture of Bacillus megaterium. Various efforts have been made to clarify the synergic pattern of this artificial microbial community and to improve the growth and production ability of K. vulgare, but there is still no sound explanation. In previous research, we found that the addition of reduced glutathione into K. vulgare monoculture could significantly improve its growth and productivity. By performing SEM and TEM, we observed that after adding GSH into K. vulgare monoculture, cells became about 4-6 folds elongated, and formed intracytoplasmic membranes (ICM. To explore the molecular mechanism and provide insights into the investigation of the synergic pattern of the co-culture system, we conducted a comparative iTRAQ-2-D-LC-MS/MS-based proteomic analysis of K. vulgare grown under reduced glutathione. Principal component analysis of proteomic data showed that after the addition of glutathione, proteins for thiamin/thiamin pyrophosphate (TPP transport, glutathione transport and the maintenance of membrane integrity, together with several membrane-bound dehydrogenases had significant up-regulation. Besides, several proteins participating in the pentose phosphate pathway and tricarboxylic acid cycle were also up-regulated. Additionally, proteins combating intracellular reactive oxygen species were also up-regulated, which similarly occurred in K. vulgare when the co-cultured B. megaterium cells lysed from our former research results. This study reveals the demand for transmembrane transport of substrates, especially thiamin, and the demand for antioxidant protection of K. vulgare.

  7. Interspecies insertion polymorphism analysis reveals recent activity of transposable elements in extant coelacanths.

    Science.gov (United States)

    Naville, Magali; Chalopin, Domitille; Volff, Jean-Nicolas

    2014-01-01

    Coelacanths are lobe-finned fish represented by two extant species, Latimeria chalumnae in South Africa and Comoros and L. menadoensis in Indonesia. Due to their intermediate phylogenetic position between ray-finned fish and tetrapods in the vertebrate lineage, they are of great interest from an evolutionary point of view. In addition, extant specimens look similar to 300 million-year-old fossils; because of their apparent slowly evolving morphology, coelacanths have been often described as « living fossils ». As an underlying cause of such a morphological stasis, several authors have proposed a slow evolution of the coelacanth genome. Accordingly, sequencing of the L. chalumnae genome has revealed a globally low substitution rate for protein-coding regions compared to other vertebrates. However, genome and gene evolution can also be influenced by transposable elements, which form a major and dynamic part of vertebrate genomes through their ability to move, duplicate and recombine. In this work, we have searched for evidence of transposition activity in coelacanth genomes through the comparative analysis of orthologous genomic regions from both Latimeria species. Comparison of 5.7 Mb (0.2%) of the L. chalumnae genome with orthologous Bacterial Artificial Chromosome clones from L. menadoensis allowed the identification of 27 species-specific transposable element insertions, with a strong relative contribution of CR1 non-LTR retrotransposons. Species-specific homologous recombination between the long terminal repeats of a new coelacanth endogenous retrovirus was also detected. Our analysis suggests that transposon activity is responsible for at least 0.6% of genome divergence between both Latimeria species. Taken together, this study demonstrates that coelacanth genomes are not evolutionary inert: they contain recently active transposable elements, which have significantly contributed to post-speciation genome divergence in Latimeria.

  8. Interspecies insertion polymorphism analysis reveals recent activity of transposable elements in extant coelacanths.

    Directory of Open Access Journals (Sweden)

    Magali Naville

    Full Text Available Coelacanths are lobe-finned fish represented by two extant species, Latimeria chalumnae in South Africa and Comoros and L. menadoensis in Indonesia. Due to their intermediate phylogenetic position between ray-finned fish and tetrapods in the vertebrate lineage, they are of great interest from an evolutionary point of view. In addition, extant specimens look similar to 300 million-year-old fossils; because of their apparent slowly evolving morphology, coelacanths have been often described as « living fossils ». As an underlying cause of such a morphological stasis, several authors have proposed a slow evolution of the coelacanth genome. Accordingly, sequencing of the L. chalumnae genome has revealed a globally low substitution rate for protein-coding regions compared to other vertebrates. However, genome and gene evolution can also be influenced by transposable elements, which form a major and dynamic part of vertebrate genomes through their ability to move, duplicate and recombine. In this work, we have searched for evidence of transposition activity in coelacanth genomes through the comparative analysis of orthologous genomic regions from both Latimeria species. Comparison of 5.7 Mb (0.2% of the L. chalumnae genome with orthologous Bacterial Artificial Chromosome clones from L. menadoensis allowed the identification of 27 species-specific transposable element insertions, with a strong relative contribution of CR1 non-LTR retrotransposons. Species-specific homologous recombination between the long terminal repeats of a new coelacanth endogenous retrovirus was also detected. Our analysis suggests that transposon activity is responsible for at least 0.6% of genome divergence between both Latimeria species. Taken together, this study demonstrates that coelacanth genomes are not evolutionary inert: they contain recently active transposable elements, which have significantly contributed to post-speciation genome divergence in Latimeria.

  9. Complexity of free energy landscapes of peptides revealed by nonlinear principal component analysis.

    Science.gov (United States)

    Nguyen, Phuong H

    2006-12-01

    Employing the recently developed hierarchical nonlinear principal component analysis (NLPCA) method of Saegusa et al. (Neurocomputing 2004;61:57-70 and IEICE Trans Inf Syst 2005;E88-D:2242-2248), the complexities of the free energy landscapes of several peptides, including triglycine, hexaalanine, and the C-terminal beta-hairpin of protein G, were studied. First, the performance of this NLPCA method was compared with the standard linear principal component analysis (PCA). In particular, we compared two methods according to (1) the ability of the dimensionality reduction and (2) the efficient representation of peptide conformations in low-dimensional spaces spanned by the first few principal components. The study revealed that NLPCA reduces the dimensionality of the considered systems much better, than did PCA. For example, in order to get the similar error, which is due to representation of the original data of beta-hairpin in low dimensional space, one needs 4 and 21 principal components of NLPCA and PCA, respectively. Second, by representing the free energy landscapes of the considered systems as a function of the first two principal components obtained from PCA, we obtained the relatively well-structured free energy landscapes. In contrast, the free energy landscapes of NLPCA are much more complicated, exhibiting many states which are hidden in the PCA maps, especially in the unfolded regions. Furthermore, the study also showed that many states in the PCA maps are mixed up by several peptide conformations, while those of the NLPCA maps are more pure. This finding suggests that the NLPCA should be used to capture the essential features of the systems.

  10. Gene coexpression analysis reveals complex metabolism of the monoterpene alcohol linalool in Arabidopsis flowers.

    Science.gov (United States)

    Ginglinger, Jean-François; Boachon, Benoit; Höfer, René; Paetz, Christian; Köllner, Tobias G; Miesch, Laurence; Lugan, Raphael; Baltenweck, Raymonde; Mutterer, Jérôme; Ullmann, Pascaline; Beran, Franziska; Claudel, Patricia; Verstappen, Francel; Fischer, Marc J C; Karst, Francis; Bouwmeester, Harro; Miesch, Michel; Schneider, Bernd; Gershenzon, Jonathan; Ehlting, Jürgen; Werck-Reichhart, Danièle

    2013-11-01

    The cytochrome P450 family encompasses the largest family of enzymes in plant metabolism, and the functions of many of its members in Arabidopsis thaliana are still unknown. Gene coexpression analysis pointed to two P450s that were coexpressed with two monoterpene synthases in flowers and were thus predicted to be involved in monoterpenoid metabolism. We show that all four selected genes, the two terpene synthases (TPS10 and TPS14) and the two cytochrome P450s (CYP71B31 and CYP76C3), are simultaneously expressed at anthesis, mainly in upper anther filaments and in petals. Upon transient expression in Nicotiana benthamiana, the TPS enzymes colocalize in vesicular structures associated with the plastid surface, whereas the P450 proteins were detected in the endoplasmic reticulum. Whether they were expressed in Saccharomyces cerevisiae or in N. benthamiana, the TPS enzymes formed two different enantiomers of linalool: (-)-(R)-linalool for TPS10 and (+)-(S)-linalool for TPS14. Both P450 enzymes metabolize the two linalool enantiomers to form different but overlapping sets of hydroxylated or epoxidized products. These oxygenated products are not emitted into the floral headspace, but accumulate in floral tissues as further converted or conjugated metabolites. This work reveals complex linalool metabolism in Arabidopsis flowers, the ecological role of which remains to be determined.

  11. Genomic analysis reveals the molecular basis for capsule loss in the group B Streptococcus population.

    Directory of Open Access Journals (Sweden)

    Roberto Rosini

    Full Text Available The human and bovine bacterial pathogen Streptococcus agalactiae (Group B Streptococcus, GBS expresses a thick polysaccharide capsule that constitutes a major virulence factor and vaccine target. GBS can be classified into ten distinct serotypes differing in the chemical composition of their capsular polysaccharide. However, non-typeable strains that do not react with anti-capsular sera are frequently isolated from colonized and infected humans and cattle. To gain a comprehensive insight into the molecular basis for the loss of capsule expression in GBS, a collection of well-characterized non-typeable strains was investigated by genome sequencing. Genome based phylogenetic analysis extended to a wide population of sequenced strains confirmed the recently observed high clonality among GBS lineages mainly containing human strains, and revealed a much higher degree of diversity in the bovine population. Remarkably, non-typeable strains were equally distributed in all lineages. A number of distinct mutations in the cps operon were identified that were apparently responsible for inactivation of capsule synthesis. The most frequent genetic alterations were point mutations leading to stop codons in the cps genes, and the main target was found to be cpsE encoding the portal glycosyl transferase of capsule biosynthesis. Complementation of strains carrying missense mutations in cpsE with a wild-type gene restored capsule expression allowing the identification of amino acid residues essential for enzyme activity.

  12. Biometric variability of goat populations revealed by means of principal component analysis.

    Science.gov (United States)

    Pires, Luanna Chácara; Machado, Théa M Medeiros; Araújo, Adriana Mello; Olson, Timothy A; da Silva, João Batista Lopes; Torres, Robledo Almeida; Costa, Márcio da Silva

    2012-12-01

    The aim was to analyze variation in 12 Brazilian and Moroccan goat populations, and, through principal component analysis (PCA), check the importance of body measures and their indices as a means of distinguishing among individuals and populations. The biometric measurements were wither height (WH), brisket height (BH) and ear length (EL). Thorax depth (WH-BH) and the three indices, TD/WH, EL/TD and EL/WH, were also calculated. Of the seven components extracted, the first three principal components were sufficient to explain 99.5% of the total variance of the data. Graphical dispersion by genetic groups revealed that European dairy breeds clustered together. The Moroccan breeds were separated into two groups, one comprising the Drâa and the other the Zagora and Rhâali breeds. Whereas, on the one side, the Anglo-Nubian and undefined breeds were the closest to one another the goats of the Azul were observed to have the highest variation of all the breeds. The Anglo-Nubian and Boer breeds were similar to each other. The Nambi-type goats remained distinct from all the other populations. In general, the use of graphical representation of PCA values allowed to distinguish genetic groups.

  13. Biometric variability of goat populations revealed by means of principal component analysis

    Directory of Open Access Journals (Sweden)

    Luanna Chácara Pires

    2012-01-01

    Full Text Available The aim was to analyze variation in 12 Brazilian and Moroccan goat populations, and, through principal component analysis (PCA, check the importance of body measures and their indices as a means of distinguishing among individuals and populations. The biometric measurements were wither height (WH, brisket height (BH and ear length (EL. Thorax depth (WH-BH and the three indices, TD/WH, EL/TD and EL/WH, were also calculated. Of the seven components extracted, the first three principal components were sufficient to explain 99.5% of the total variance of the data. Graphical dispersion by genetic groups revealed that European dairy breeds clustered together. The Moroccan breeds were separated into two groups, one comprising the Drâa and the other the Zagora and Rhâali breeds. Whereas, on the one side, the Anglo-Nubian and undefined breeds were the closest to one another the goats of the Azul were observed to have the highest variation of all the breeds. The Anglo-Nubian and Boer breeds were similar to each other. The Nambi-type goats remained distinct from all the other populations. In general, the use of graphical representation of PCA values allowed to distinguish genetic groups.

  14. Retrospective stable isotope analysis reveals ecosystem responses to river regulation over the last century.

    Science.gov (United States)

    Turner, Thomas F; Krabbenhoft, Trevor I; Collyer, Michael L; Krabbenhoft, Corey A; Edwards, Melanie S; Sharp, Zachary D

    2015-12-01

    Disruption of natural flow regimes, nutrient pollution, and other consequences of human population growth and development have impacted most major rivers of the world. Alarming losses of aquatic biodiversity coincide with human-caused river alteration, but effects of biotic homogenization on aquatic ecosystem processes are not as well documented. This is because unaltered systems for comparison are scarce, and some ecosystem-wide effects may take decades to manifest. We evaluated aquatic ecosystem responses to extensive river- floodplain engineering and nutrient addition in the Rio Grande of southwestern North America as revealed by changes in trophic structure of, and resource availability to, the fish community. Stable Isotope Analysis (SIA) was conducted on museum-preserved fishes collected over a 70-year period of intensive river management and exponential human population growth. Trophic complexity and resource heterogeneity for fish consumers (measured as "isotopic niche breadth") decreased following sediment deprivation and channelization, and these effects persist into the present. Increased nutrient inputs led to δ15N enrichment in the entire fish community at all affected sites, and a shift to autochthonous sources of carbon at the most proximal site downstream of wastewater release, probably via bottom-up transfer. Overall, retrospective SIA of apex consumers suggests radical change and functional impairment of a floodplain river ecosystem already marked by significant biodiversity loss.

  15. Phylum-specific environmental DNA analysis reveals remarkably high global biodiversity of Cercozoa (Protozoa).

    Science.gov (United States)

    Bass, David; Cavalier-Smith, Thomas

    2004-11-01

    This study presents the first 18S rRNA multi-library environmental PCR survey of a single protozoan phylum, Cercozoa Cavalier-Smith 1998, from a range of different habitats. Phylogenetic analysis reveals at least nine novel clades within the phylum, several possibly at the level of order or above. Further experiments are described to ascertain the true ecological and geographical distributions of some clades that might be inferred from the tree to be restricted in either or both ways. These results suggest that the diversity of cercozoan taxa may run into thousands of lineages, making it comparable in diversity to the largest better-characterized protozoan phyla, e.g. Ciliophora (ciliates and suctorians) and Foraminifera. New sequences of cultured Spongomonas, Metromonas and Metopion are also presented. In the light of these additions, and the increased taxon sampling from the environmental libraries, some revisions of cercozoan classification are made: the transfer of Spongomonadea from Reticulofilosa to Monadofilosa; the removal of Metopiida from Sarcomonadea; and the creation of the new order Metromonadida, currently containing the single genus Metromonas. Although Metromonas groups with weak to moderate support with Chlorarachnea, it is here placed in superclass Monadofilosa, to which it is morphologically more similar.

  16. Transcriptome analysis reveals positive selection on the divergent between topmouth culter and zebrafish.

    Science.gov (United States)

    Ren, Li; Tan, Xing-Jun; Xiong, Ya-Feng; Xu, Kang; Zhou, Yi; Zhong, Huan; Liu, Yun; Hong, Yun-Han; Liu, Shao-Jun

    2014-12-01

    The topmouth culter (Erythroculter ilishaeformis) is a predatory cyprinid fish that distributes widely in the East Asia. Here we report the liver transcriptome in this organism as a model of predatory fish. Sequencing of 5 Gb raw reads led to 27,741 unigenes and produced 11,131 annotatable genes. A total of 7093 (63.7%) genes were found to have putative functions by gene ontology analysis. Importantly, a blast search revealed 4033 culter genes that were orthologous to the zebrafish. Extracted from 38 candidate positive selection genes, 4 genes exhibit strong positive selection based on the ratio of nonsynonymous (Ka) to synonymous substitutions (Ks). In addition, the four genes also indicated the strong positive selection by comparing them between blunt snout bream (Megalobrama amblycephala) and zebrafish. These genes were involved in activator of gene expression, metabolic processes and development. The transcriptome variation may be reflective of natural selection in the early life history of Cyprinidae. Based on Ks ratios, date of the separation between topmouth culter and zebrafish is approximately 64 million years ago. We conclude that natural selection acts in diversifying the genomes between topmouth culter and zebrafish.

  17. Genome-wide analysis reveals specificities of Cpf1 endonucleases in human cells.

    Science.gov (United States)

    Kim, Daesik; Kim, Jungeun; Hur, Junho K; Been, Kyung Wook; Yoon, Sun-Heui; Kim, Jin-Soo

    2016-08-01

    Programmable clustered regularly interspaced short palindromic repeats (CRISPR) Cpf1 endonucleases are single-RNA-guided (crRNA) enzymes that recognize thymidine-rich protospacer-adjacent motif (PAM) sequences and produce cohesive double-stranded breaks (DSBs). Genome editing with CRISPR-Cpf1 endonucleases could provide an alternative to CRISPR-Cas9 endonucleases, but the determinants of targeting specificity are not well understood. Using mismatched crRNAs we found that Cpf1 could tolerate single or double mismatches in the 3' PAM-distal region, but not in the 5' PAM-proximal region. Genome-wide analysis of cleavage sites in vitro for eight Cpf1 nucleases using Digenome-seq revealed that there were 6 (LbCpf1) and 12 (AsCpf1) cleavage sites per crRNA in the human genome, fewer than are present for Cas9 nucleases (>90). Most Cpf1 off-target cleavage sites did not produce mutations in cells. We found mismatches in either the 3' PAM-distal region or in the PAM sequence of 12 off-target sites that were validated in vivo. Off-target effects were completely abrogated by using preassembled, recombinant Cpf1 ribonucleoproteins.

  18. Lipidomic profiling analysis reveals the dynamics of phospholipid molecules in Arabidopsis thaliana seedling growth

    Institute of Scientific and Technical Information of China (English)

    Yi-Sheng Wang; Hong-Yan Yao; Hong-Wei Xue

    2016-01-01

    High-throughput lipidomic profiling provides a sensitive approach for discovering minor lipid species. By using an advance in electrospray ionization tandem mass spectrome-try, a large set of phospholipid molecular species (126 species) with high resolution were identified from Arabidopsis seedling;of them 31 species are newly identified (16 are unique in plants), including 13 species of phosphatidic acid (PA), nine phosphati-dylcholine, six phosphatidylinositol and three phosphatidylser-ine. Further analysis of the lipidomic profile reveals dynamics of phospholipids and distinct species alterations during seedling development. PA molecules are found at the lowest levels in imbibition and fol ow an increasing trend during seedling growth, while phosphatidylethanolamine (PE) molecules show the opposite pattern with highest levels at imbibition and a general decreasing trend at later stages. Of PA molecular species, 34:2-, 34:3-, 36:4-, 36:5-, 38:3- and 38:4-PA increase during radicle emergence, and 34:2-and 34:3-PA reach highest levels during hypocotyl and cotyledon emergence from the seed coat. Conversely, molecular species of PE show higher levels in imbibition and decrease in later stages. These results suggest the crucial roles of specific molecular species and homeostasis of phospholipid molecules in seedling growth and provide insights into the mechanisms of how phospholipid molecules are involved in regulating plant development.

  19. Genome-Wide Analysis Revealed the Complex Regulatory Network of Brassinosteroid Effects in Photomorphogenesis

    Institute of Scientific and Technical Information of China (English)

    Li Song; Xiao-Yi Zhou; Li Li; Liang-Jiao Xue; Xi Yang; Hong-Wei Xue

    2009-01-01

    Light and brassinosteroids (BRs) have been proved to be crucial in regulating plant growth and development;however,the mechanism of how they synergistically function is still largely unknown.To explore the underlying mechanisms in photomorphogenesis,genome-wide analyses were carried out through examining the gene expressions of the dark-grown WT or BR biosynthesis-defective mutant det2 seedlings in the presence of light stimuli or exogenous Brassinolide (BL).Results showed that BR deficiency stimulates,while BL treatment suppresses,the expressions of lightresponsive genes and photomorphogenesis,confirming the negative effects of BR in photomorphogenesis.This is consistent with the specific effects of BR on the expression of genes involved in cell wall modification,cellular metabolism and energy utilization during dark-light transition.Further analysis revealed that hormone biosynthesis and signaling-related genes,especially those of auxin,were altered under BL treatment or light stimuli,indicating that BR may modulate photomorphogenesis through synergetic regulation with other hormones.Additionally,suppressed ubiquitin-cycle pathway during light-dark transition hinted the presence of a complicated network among light,hormone,and protein degradation.The study provides the direct evidence of BR effects in photomorphogenesis and identified the genes involved in BR and light signaling pathway,which will help to elucidate the molecular mechanism of plant photomorphogenesis.

  20. Transcriptional analysis of the endothelial response to diabetes reveals a role for galectin-3.

    Science.gov (United States)

    Darrow, April L; Shohet, Ralph V; Maresh, J Gregory

    2011-10-20

    To characterize the endothelial dysfunction associated with Type II diabetes, we surveyed transcriptional responses in the vascular endothelia of mice receiving a diabetogenic, high-fat diet. Tie2-GFP mice were fed a diet containing 60% fat calories (HFD); controls were littermates fed normal chow. Following 4, 6, and 8 wk, aortic and leg muscle tissues were enzymatically dispersed, and endothelial cells were obtained by fluorescence-activated cell sorting. Relative mRNA abundance in HFD vs. control endothelia was measured with long-oligo microarrays; highly dysregulated genes were confirmed by real-time PCR and protein quantification. HFD mice were hyperglycemic by 2 wk and displayed vascular insulin resistance and decreased glucose tolerance by 5 and 6 wk, respectively. Endothelial transcripts upregulated by HFD included galectin-3 (Lgals3), 5-lipoxygenase-activating protein, and chemokine ligands 8 and 9. Increased LGALS3 protein was detected in muscle endothelium by immunohistology accompanied by elevated LGALS3 in the serum of HFD mice. Our comprehensive analysis of the endothelial transcriptional response in a model of Type II diabetes reveals novel regulation of transcripts with roles in inflammation, insulin sensitivity, oxidative stress, and atherosclerosis. Increased endothelial expression and elevated humoral levels of LGALS3 supports a role for this molecule in the vascular response to diabetes, and its potential as a direct biomarker for the inflammatory state in diabetes.

  1. A quorum-sensing factor in vegetative Dictyostelium discoideum cells revealed by quantitative migration analysis.

    Directory of Open Access Journals (Sweden)

    Laurent Golé

    Full Text Available BACKGROUND: Many cells communicate through the production of diffusible signaling molecules that accumulate and once a critical concentration has been reached, can activate or repress a number of target genes in a process termed quorum sensing (QS. In the social amoeba Dictyostelium discoideum, QS plays an important role during development. However little is known about its effect on cell migration especially in the growth phase. METHODS AND FINDINGS: To investigate the role of cell density on cell migration in the growth phase, we use multisite timelapse microscopy and automated cell tracking. This analysis reveals a high heterogeneity within a given cell population, and the necessity to use large data sets to draw reliable conclusions on cell motion. In average, motion is persistent for short periods of time (t ≤ 5 min, but normal diffusive behavior is recovered over longer time periods. The persistence times are positively correlated with the migrated distances. Interestingly, the migrated distance decreases as well with cell density. The adaptation of cell migration to cell density highlights the role of a secreted quorum sensing factor (QSF on cell migration. Using a simple model describing the balance between the rate of QSF generation and the rate of QSF dilution, we were able to gather all experimental results into a single master curve, showing a sharp cell transition between high and low motile behaviors with increasing QSF. CONCLUSION: This study unambiguously demonstrates the central role played by QSF on amoeboid motion in the growth phase.

  2. Genomic analysis reveals the molecular basis for capsule loss in the group B Streptococcus population.

    Science.gov (United States)

    Rosini, Roberto; Campisi, Edmondo; De Chiara, Matteo; Tettelin, Hervé; Rinaudo, Daniela; Toniolo, Chiara; Metruccio, Matteo; Guidotti, Silvia; Sørensen, Uffe B Skov; Kilian, Mogens; Ramirez, Mario; Janulczyk, Robert; Donati, Claudio; Grandi, Guido; Margarit, Immaculada

    2015-01-01

    The human and bovine bacterial pathogen Streptococcus agalactiae (Group B Streptococcus, GBS) expresses a thick polysaccharide capsule that constitutes a major virulence factor and vaccine target. GBS can be classified into ten distinct serotypes differing in the chemical composition of their capsular polysaccharide. However, non-typeable strains that do not react with anti-capsular sera are frequently isolated from colonized and infected humans and cattle. To gain a comprehensive insight into the molecular basis for the loss of capsule expression in GBS, a collection of well-characterized non-typeable strains was investigated by genome sequencing. Genome based phylogenetic analysis extended to a wide population of sequenced strains confirmed the recently observed high clonality among GBS lineages mainly containing human strains, and revealed a much higher degree of diversity in the bovine population. Remarkably, non-typeable strains were equally distributed in all lineages. A number of distinct mutations in the cps operon were identified that were apparently responsible for inactivation of capsule synthesis. The most frequent genetic alterations were point mutations leading to stop codons in the cps genes, and the main target was found to be cpsE encoding the portal glycosyl transferase of capsule biosynthesis. Complementation of strains carrying missense mutations in cpsE with a wild-type gene restored capsule expression allowing the identification of amino acid residues essential for enzyme activity.

  3. Genome-wide analysis of homeobox genes from Mesobuthus martensii reveals Hox gene duplication in scorpions.

    Science.gov (United States)

    Di, Zhiyong; Yu, Yao; Wu, Yingliang; Hao, Pei; He, Yawen; Zhao, Huabin; Li, Yixue; Zhao, Guoping; Li, Xuan; Li, Wenxin; Cao, Zhijian

    2015-06-01

    Homeobox genes belong to a large gene group, which encodes the famous DNA-binding homeodomain that plays a key role in development and cellular differentiation during embryogenesis in animals. Here, one hundred forty-nine homeobox genes were identified from the Asian scorpion, Mesobuthus martensii (Chelicerata: Arachnida: Scorpiones: Buthidae) based on our newly assembled genome sequence with approximately 248 × coverage. The identified homeobox genes were categorized into eight classes including 82 families: 67 ANTP class genes, 33 PRD genes, 11 LIM genes, five POU genes, six SINE genes, 14 TALE genes, five CUT genes, two ZF genes and six unclassified genes. Transcriptome data confirmed that more than half of the genes were expressed in adults. The homeobox gene diversity of the eight classes is similar to the previously analyzed Mandibulata arthropods. Interestingly, it is hypothesized that the scorpion M. martensii may have two Hox clusters. The first complete genome-wide analysis of homeobox genes in Chelicerata not only reveals the repertoire of scorpion, arachnid and chelicerate homeobox genes, but also shows some insights into the evolution of arthropod homeobox genes.

  4. GC--MS analysis reveals production of 2--Phenylethanol from Aspergillus niger endophytic in rose.

    Science.gov (United States)

    Wani, Masood Ahmed; Sanjana, Kaul; Kumar, Dhar Manoj; Lal, Dhar Kanahya

    2010-02-01

    Endophytes include all organisms that during a variable period of their life, colonize the living internal tissues of their hosts without causing detectable symptoms. Several fungal endophytes have been isolated from a variety of plant species which have proved themselves as a rich source of secondary metabolites. The reported natural products from endophytes include antibiotics, immunosuppresants, anticancer compounds, antioxidant agents, etc. For the first time Rosa damacaena (rose) has been explored for its endophytes. The rose oil industry is the major identified deligence for its application in perfumery, flavouring, ointments, and pharmaceuticals including various herbal products. During the present investigation fungal endophytes were isolated from Rosa damacaena. A total of fifty four isolates were isolated out of which sixteen isolates were screened for the production of secondary metabolites. GCMS analysis reveals the production of 2-phenylethanol by one of the isolates JUBT 3M which was identified as Aspergillus niger. This is the first report of production of 2-phenylethanol from endophytic A. niger. 2-phenylethanol is an important constituent of rose oil constituting about 4.06% of rose oil. Presence of 2-phenylethanol indicates that the endophyte of rose may duplicate the biosynthesis of phenyl propanoids by rose plant. Besides this, the other commercial applications of phenylethanol include its use in antiseptics, disinfectants, anti-microbials and preservative in pharmaceuticals.

  5. RNA-Seq Analysis Reveals Candidate Targets for Curcumin against Tetranychus cinnabarinus

    Directory of Open Access Journals (Sweden)

    Xuejiao Liu

    2016-01-01

    Full Text Available Tetranychus cinnabarinus is an important agricultural pest with a broad host range. We previously identified curcumin as a promising acaricidal compound against T. cinnabarinus. However, the acaricidal mechanism of curcumin remains unknown. In this study, RNA-seq was employed to analyze the transcriptome changes in T. cinnabarinus treated with curcumin or the solvent. A total of 105,706,297 clean sequence reads were generated by sequencing, with more than 90% of the reads successfully mapped to the reference sequence. The RNA-seq identified 111 and 96 differentially expressed genes between curcumin- and solvent-treated mites at 24 and 48 h after treatment, respectively. GO enrichment analysis of differentially expressed genes showed that the cellular process was the dominant group at both time points. Finally, we screened 23 differentially expressed genes that were functionally identical or similar to the targets of common insecticide/acaricides or genes that were associated with mite detoxification and metabolism. Calmodulin, phospholipase A2, and phospholipase C were activated upon curcumin treatment suggesting that the calcium channel related genes might play important roles in mite’s response to curcumin. Overall our results revealed the global transcriptional changes in T. cinnabarinus after curcumin treatment to enable further identification of the targets of curcumin in mites.

  6. Latent physiological factors of complex human diseases revealed by independent component analysis of clinarrays

    Directory of Open Access Journals (Sweden)

    Chen David P

    2010-10-01

    Full Text Available Abstract Background Diagnosis and treatment of patients in the clinical setting is often driven by known symptomatic factors that distinguish one particular condition from another. Treatment based on noticeable symptoms, however, is limited to the types of clinical biomarkers collected, and is prone to overlooking dysfunctions in physiological factors not easily evident to medical practitioners. We used a vector-based representation of patient clinical biomarkers, or clinarrays, to search for latent physiological factors that underlie human diseases directly from clinical laboratory data. Knowledge of these factors could be used to improve assessment of disease severity and help to refine strategies for diagnosis and monitoring disease progression. Results Applying Independent Component Analysis on clinarrays built from patient laboratory measurements revealed both known and novel concomitant physiological factors for asthma, types 1 and 2 diabetes, cystic fibrosis, and Duchenne muscular dystrophy. Serum sodium was found to be the most significant factor for both type 1 and type 2 diabetes, and was also significant in asthma. TSH3, a measure of thyroid function, and blood urea nitrogen, indicative of kidney function, were factors unique to type 1 diabetes respective to type 2 diabetes. Platelet count was significant across all the diseases analyzed. Conclusions The results demonstrate that large-scale analyses of clinical biomarkers using unsupervised methods can offer novel insights into the pathophysiological basis of human disease, and suggest novel clinical utility of established laboratory measurements.

  7. Temporal proteomic analysis reveals continuous impairment of intestinal development in neonatal piglets with intrauterine growth restriction.

    Science.gov (United States)

    Wang, Xiaoqiu; Wu, Weizong; Lin, Gang; Li, Defa; Wu, Guoyao; Wang, Junjun

    2010-02-01

    Efficiency of nutrient utilization is reduced in neonates with intrauterine growth restriction (IUGR) compared with those with a normal birth weight (NBW). However, the underlying mechanisms are largely unknown. In this study, we applied temporal proteomic approach, coupled with histological and biochemical analyses, to study dynamic changes of the proteome in the small intestinal mucosa of IUGR piglets during the nursing period (Days 1, 7 and 21). We identified 56 differentially expressed protein spots between IUGR and NBW piglets. These proteins participate in key biological processes, including (1) absorption, digestion and transport of nutrients; (2) cell structure and motility; (3) glucose and energy metabolism; (4) lipid metabolism; (5) amino acid metabolism; (6) mineral and vitamin metabolism; (7) cellular redox homeostasis; (8) stress response; and (9) apoptosis. The results of our temporal proteomics analysis reveal continuous impairment of intestinal development in neonatal piglets with IUGR. The findings have important implications for understanding metabolic defects in the small intestine of IUGR neonates and are expected to provide new strategies to improve their survival and growth.

  8. RNA sequencing analysis of human podocytes reveals glucocorticoid regulated gene networks targeting non-immune pathways

    Science.gov (United States)

    Jiang, Lulu; Hindmarch, Charles C. T.; Rogers, Mark; Campbell, Colin; Waterfall, Christy; Coghill, Jane; Mathieson, Peter W.; Welsh, Gavin I.

    2016-01-01

    Glucocorticoids are steroids that reduce inflammation and are used as immunosuppressive drugs for many diseases. They are also the mainstay for the treatment of minimal change nephropathy (MCN), which is characterised by an absence of inflammation. Their mechanisms of action remain elusive. Evidence suggests that immunomodulatory drugs can directly act on glomerular epithelial cells or ‘podocytes’, the cell type which is the main target of injury in MCN. To understand the nature of glucocorticoid effects on non-immune cell functions, we generated RNA sequencing data from human podocyte cell lines and identified the genes that are significantly regulated in dexamethasone-treated podocytes compared to vehicle-treated cells. The upregulated genes are of functional relevance to cytoskeleton-related processes, whereas the downregulated genes mostly encode pro-inflammatory cytokines and growth factors. We observed a tendency for dexamethasone-upregulated genes to be downregulated in MCN patients. Integrative analysis revealed gene networks composed of critical signaling pathways that are likely targeted by dexamethasone in podocytes. PMID:27774996

  9. Highly distinct chromosomal structures in cowpea (Vigna unguiculata), as revealed by molecular cytogenetic analysis.

    Science.gov (United States)

    Iwata-Otsubo, Aiko; Lin, Jer-Young; Gill, Navdeep; Jackson, Scott A

    2016-05-01

    Cowpea (Vigna unguiculata (L.) Walp) is an important legume, particularly in developing countries. However, little is known about its genome or chromosome structure. We used molecular cytogenetics to characterize the structure of pachytene chromosomes to advance our knowledge of chromosome and genome organization of cowpea. Our data showed that cowpea has highly distinct chromosomal structures that are cytologically visible as brightly DAPI-stained heterochromatic regions. Analysis of the repetitive fraction of the cowpea genome present at centromeric and pericentromeric regions confirmed that two retrotransposons are major components of pericentromeric regions and that a 455-bp tandem repeat is found at seven out of 11 centromere pairs in cowpea. These repeats likely evolved after the divergence of cowpea from common bean and form chromosomal structure unique to cowpea. The integration of cowpea genetic and physical chromosome maps reveals potential regions of suppressed recombination due to condensed heterochromatin and a lack of pairing in a few chromosomal termini. This study provides fundamental knowledge on cowpea chromosome structure and molecular cytogenetics tools for further chromosome studies.

  10. Organizational frame of environmental policy convergence of Ukraine with EU

    Directory of Open Access Journals (Sweden)

    Orlovska Juliia Valeriivna

    2014-12-01

    Full Text Available The aim of the article. The aim of the article is to elaborate organizational frame of environmental policy convergence of Ukraine with EU. The emphasis was placed on one of the goals of Ukraine-European Union Association Agreement, in particular: “to establish conditions for strengthening economic and trade relations, which will lead to the gradual integration Ukraine in the EU internal market”. It has been revealed that the primary constraints that hinder deepening trade relationships between Ukraine and the EU are factors, which by their nature relate to structural and technological aspects of Ukrainian economy. The results of the analysis. The effectiveness of harmonization process can be tracked through the progress in implementing the priorities agenda EU-Ukraine Association. The data of 2013 evidence that Ukraine is gradually moving towards the implementation of the international conventions. A considerable attention should be paid to the progress in diffusion process, because it greatly contributes to the development and applying innovations. The latter is on the agenda for Ukraine as it can solve both the problems concerning technology and environmental effectiveness. Another fundamental problem of poor ecological condition in Ukraine could be seen in uneven spatial concentration of production. Indeed, the present conditions of sector entities activities create significant anthropogenic environmental change, its degradation and destruction due to a combination of large amounts of industrial and agricultural production in some areas and the same. It should be noted that the influence of public on environmental decision-making is absent. Thus, the Law “On regulation of urban development” doesn't realize compliance with the relevant legal procedures by which public participation would be determined in the decision-making on urban development, that in turn eliminates the provisions of the Aarhus Convention “On Access to

  11. Blind image deconvolution methods and convergence

    CERN Document Server

    Chaudhuri, Subhasis; Rameshan, Renu

    2014-01-01

    Blind deconvolution is a classical image processing problem which has been investigated by a large number of researchers over the last four decades. The purpose of this monograph is not to propose yet another method for blind image restoration. Rather the basic issue of deconvolvability has been explored from a theoretical view point. Some authors claim very good results while quite a few claim that blind restoration does not work. The authors clearly detail when such methods are expected to work and when they will not. In order to avoid the assumptions needed for convergence analysis in the

  12. The Immersive Virtual Reality Experience: A Typology of Users Revealed Through Multiple Correspondence Analysis Combined with Cluster Analysis Technique.

    Science.gov (United States)

    Rosa, Pedro J; Morais, Diogo; Gamito, Pedro; Oliveira, Jorge; Saraiva, Tomaz

    2016-03-01

    Immersive virtual reality is thought to be advantageous by leading to higher levels of presence. However, and despite users getting actively involved in immersive three-dimensional virtual environments that incorporate sound and motion, there are individual factors, such as age, video game knowledge, and the predisposition to immersion, that may be associated with the quality of virtual reality experience. Moreover, one particular concern for users engaged in immersive virtual reality environments (VREs) is the possibility of side effects, such as cybersickness. The literature suggests that at least 60% of virtual reality users report having felt symptoms of cybersickness, which reduces the quality of the virtual reality experience. The aim of this study was thus to profile the right user to be involved in a VRE through head-mounted display. To examine which user characteristics are associated with the most effective virtual reality experience (lower cybersickness), a multiple correspondence analysis combined with cluster analysis technique was performed. Results revealed three distinct profiles, showing that the PC gamer profile is more associated with higher levels of virtual reality effectiveness, that is, higher predisposition to be immersed and reduced cybersickness symptoms in the VRE than console gamer and nongamer. These findings can be a useful orientation in clinical practice and future research as they help identify which users are more predisposed to benefit from immersive VREs.

  13. Proteomic and bioinformatic analysis of epithelial tight junction reveals an unexpected cluster of synaptic molecules

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    Tang Vivian W

    2006-12-01

    Full Text Available Abstract Background Zonula occludens, also known as the tight junction, is a specialized cell-cell interaction characterized by membrane "kisses" between epithelial cells. A cytoplasmic plaque of ~100 nm corresponding to a meshwork of densely packed proteins underlies the tight junction membrane domain. Due to its enormous size and difficulties in obtaining a biochemically pure fraction, the molecular composition of the tight junction remains largely unknown. Results A novel biochemical purification protocol has been developed to isolate tight junction protein complexes from cultured human epithelial cells. After identification of proteins by mass spectroscopy and fingerprint analysis, candidate proteins are scored and assessed individually. A simple algorithm has been devised to incorporate transmembrane domains and protein modification sites for scoring membrane proteins. Using this new scoring system, a total of 912 proteins have been identified. These 912 hits are analyzed using a bioinformatics approach to bin the hits in 4 categories: configuration, molecular function, cellular function, and specialized process. Prominent clusters of proteins related to the cytoskeleton, cell adhesion, and vesicular traffic have been identified. Weaker clusters of proteins associated with cell growth, cell migration, translation, and transcription are also found. However, the strongest clusters belong to synaptic proteins and signaling molecules. Localization studies of key components of synaptic transmission have confirmed the presence of both presynaptic and postsynaptic proteins at the tight junction domain. To correlate proteomics data with structure, the tight junction has been examined using electron microscopy. This has revealed many novel structures including end-on cytoskeletal attachments, vesicles fusing/budding at the tight junction membrane domain, secreted substances encased between the tight junction kisses, endocytosis of tight junction

  14. Differential Genes Expression between Fertile and Infertile Spermatozoa Revealed by Transcriptome Analysis.

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    Sandeep Kumar Bansal

    Full Text Available It was believed earlier that spermatozoa have no traces of RNA because of loss of most of the cytoplasm. Recent studies have revealed the presence of about 3000 different kinds of mRNAs in ejaculated spermatozoa. However, the correlation of transcriptome profile with infertility remains obscure.Total RNA from sperm (after exclusion of somatic cells of 60 men consisting of individuals with known fertility (n=20, idiopathic infertility (normozoospermic patients, n=20, and asthenozoospermia (n=20 was isolated. After RNA quality check on Bioanalyzer, AffymetrixGeneChip Human Gene 1.0 ST Array was used for expression profiling, which consisted of >30,000 coding transcripts and >11,000 long intergenic non-coding transcripts.Comparison between all three groups revealed that two thousand and eighty one transcripts were differentially expressed. Analysis of these transcripts showed that some transcripts [ribosomal proteins (RPS25, RPS11, RPS13, RPL30, RPL34, RPL27, RPS5, HINT1, HSP90AB1, SRSF9, EIF4G2, ILF2] were up-regulated in the normozoospermic group, but down-regulated in the asthenozoospermic group in comparison to the control group. Some transcripts were specific to the normozoospermic group (up-regulated: CAPNS1, FAM153C, ARF1, CFL1, RPL19, USP22; down-regulated: ZNF90, SMNDC1, c14orf126, HNRNPK, while some were specific to the asthenozoospermic group (up-regulated: RPL24, HNRNPM, RPL4, PRPF8, HTN3, RPL11, RPL28, RPS16, SLC25A3, C2orf24, RHOA, GDI2, NONO, PARK7; down-regulated: HNRNPC, SMARCAD1, RPS24, RPS24, RPS27A, KIFAP3. A number of differentially expressed transcripts in spermatozoa were related to reproduction (n = 58 and development (n= 210. Some of these transcripts were related to heat shock proteins (DNAJB4, DNAJB14, testis specific genes (TCP11, TESK1, TSPYL1, ADAD1, and Y-chromosome genes (DAZ1, TSPYL1.A complex RNA population in spermatozoa consisted of coding and non-coding RNAs. A number of transcripts that participate in a host of

  15. Instability and Tsunamigenic Potential at Convergent Margins

    Science.gov (United States)

    von Huene, R.; Ranero, C. R.; Watts, P.

    2001-12-01

    Along many convergent margins multibeam echosounding navigated with GPS has revealed large slope failures that were probably tsunamigenic. Bathymetric data combined with seismic reflection imaging indicate multiple causes. The 55-km wide Nicoya Slump resulted from the steepening slope above an underthrusting seamount on the subducting oceanic plate. This slump may have generated a 27-m high wave. Several 5-7 km wide mid-slope slides off central Nicaragua probably resulted from steepening of the continental slope by tectonic erosion. They may have generated waves 6-7 m high. A 30 km wide mid-slope slump off northern Peru may have generated a 5 m high wave. Its cause will not be understood without better seismic reflection imaging but considerable fluid venting was observed across its headwall. In the Gulf of Alaska a large slide appears to have resulted from rapid sedimentation. Tsunamigenic slope failure along convergent margins is only beginning to be resolved and the causes vary. Subducted ocean floor relief, tectonically steepened slopes, and sites of rapid sedimentation can help target potential failure and possible future tsunami hazards.

  16. Proliferating Connections and Communicating Convergence

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    Aylish Wood

    2008-01-01

    Full Text Available In this paper I use the work of Niklas Luhmann to explore what pressure the concept of convergence exerts over how we communicate about the changing expressive practices of digital games following the emergence of digital technologies. My claim will be that in its current form, convergence privileges either the human users of technological platforms, or the combination of aesthetic conventions from different media what connects is either the user or the aesthetic code. While neither of these two positions would be likely to deny a reliance on the interplay between humans and technologies, such an interplay is taken for granted rather than explored. By using Luhmann’s version of systems theory, I argue that we can more effectively grasp the interplay of human and technological participants by understanding their combined roles in changing expressive practices.

  17. Concept Convergence in Empirical Domains

    Science.gov (United States)

    Ontañón, Santiago; Plaza, Enric

    How to achieve shared meaning is a significant issue when more than one intelligent agent is involved in the same domain. We define the task of concept convergence, by which intelligent agents can achieve a shared, agreed-upon meaning of a concept (restricted to empirical domains). For this purpose we present a framework that, integrating computational argumentation and inductive concept learning, allows a pair of agents to (1) learn a concept in an empirical domain, (2) argue about the concept's meaning, and (3) reach a shared agreed-upon concept definition. We apply this framework to marine sponges, a biological domain where the actual definitions of concepts such as orders, families and species are currently open to discussion. An experimental evaluation on marine sponges shows that concept convergence is achieved, within a reasonable number of interchanged arguments, and reaching short and accurate definitions (with respect to precision and recall).

  18. Evolutionary, structural and functional relationships revealed by comparative analysis of syntenic genes in Rhizobiales

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    Medrano-Soto Arturo

    2005-10-01

    Full Text Available Abstract Background Comparative genomics has provided valuable insights into the nature of gene sequence variation and chromosomal organization of closely related bacterial species. However, questions about the biological significance of gene order conservation, or synteny, remain open. Moreover, few comprehensive studies have been reported for rhizobial genomes. Results We analyzed the genomic sequences of four fast growing Rhizobiales (Sinorhizobium meliloti, Agrobacterium tumefaciens, Mesorhizobium loti and Brucella melitensis. We made a comprehensive gene classification to define chromosomal orthologs, genes with homologs in other replicons such as plasmids, and those which were species-specific. About two thousand genes were predicted to be orthologs in each chromosome and about 80% of these were syntenic. A striking gene colinearity was found in pairs of organisms and a large fraction of the microsyntenic regions and operons were similar. Syntenic products showed higher identity levels than non-syntenic ones, suggesting a resistance to sequence variation due to functional constraints; also, an unusually high fraction of syntenic products contained membranal segments. Syntenic genes encode a high proportion of essential cell functions, presented a high level of functional relationships and a very low horizontal gene transfer rate. The sequence variability of the proteins can be considered the species signature in response to specific niche adaptation. Comparatively, an analysis with genomes of Enterobacteriales showed a different gene organization but gave similar results in the synteny conservation, essential role of syntenic genes and higher functional linkage among the genes of the microsyntenic regions. Conclusion Syntenic bacterial genes represent a commonly evolved group. They not only reveal the core chromosomal segments present in the last common ancestor and determine the metabolic characteristics shared by these microorganisms

  19. Carbon sources in the Beaufort Sea revealed by molecular lipid biomarkers and compound specific isotope analysis

    Science.gov (United States)

    Tolosa, I.; Fiorini, S.; Gasser, B.; Martín, J.; Miquel, J. C.

    2012-10-01

    Molecular lipid biomarkers (hydrocarbons, alcohols, sterols and fatty acids) and compound specific isotope analysis of suspended particulate organic matter (SPM) and surface sediments of the Mackenzie Shelf and slope (Southeast Beaufort Sea, Arctic Ocean), were studied in summer 2009. The concentrations of the molecular lipid markers, characteristic of known organic matter sources, were grouped and used as proxies to evaluate the relative importance of fresh algal, detrital algal, fossil, C3 terrestrial plants, bacterial and zooplankton material in the sedimentary organic matter (OM). Fossil and detrital algal contributions were the major fractions of the freshwater SPM from the Mackenzie River with ~34% each of the total molecular biomarkers. Fresh algal, C3 terrestrial, bacterial and zooplanktonic components represented much lower percentages, 17, 10, 4 and 80%) with a minor contribution of fossil and C3 terrestrial biomarkers. Characterization of the sediments revealed a major sink of refractory algal material mixed with some fresh algal material, fossil hydrocarbons and a small input of C3 terrestrial sources. In particular, the sediments from the shelf and at the mouth of the Amundsen Gulf presented the highest contribution of detrital algal material (60-75%) whereas those from the slope contained the highest proportion of fossil (40%) and C3 terrestrial plant material (10%). Overall, considering that the detrital algal material is marine derived, autochthonous sources contributed more than allochthonous sources to the OM lipid pool. Using the ratio of an allochthonous biomarker (normalized to total organic carbon, TOC) found in the sediments to those measured at the river mouth water, we estimated that the fraction of terrestrial material preserved in the sediments accounted for 30-40% of the total carbon in the inner shelf sediments, 17% in the outer shelf and Amundsen Gulf and up to 25% in the slope sediments.

  20. Proteomic analysis reveals metabolic and regulatory systems involved the syntrophic and axenic lifestyle of Syntrophomonas wolfei.

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    Jessica Rhea Sieber

    2015-02-01

    Full Text Available Microbial syntrophy is a vital metabolic interaction necessary for the complete oxidation of organic biomass to methane in all-anaerobic ecosystems. However, this process is thermodynamically constrained and represents an ecosystem-level metabolic bottleneck. To gain insight into the physiology of this process, a shotgun proteomic approach was used to quantify the protein landscape of the model syntrophic metabolizer, Syntrophomonas wolfei, grown axenically and syntrophically with Methanospirillum hungatei. Remarkably, the abundance of most proteins as represented by normalized spectral abundance factor (NSAF value changed very little between the pure and coculture growth conditions. Among the most abundant proteins detected were GroEL and GroES chaperonins, a small heat shock protein, and proteins involved in electron transfer, beta-oxidation, and ATP synthesis. Several putative energy conservation enzyme systems that utilize NADH and ferredoxin were present. The abundance of an EtfAB2 and the membrane-bound iron-sulfur oxidoreductase (Swol_0698 gene product delineated a potential conduit for electron transfer between acyl-CoA dehydrogenases and membrane redox carriers. Proteins detected only when S. wolfei was grown with M. hungatei included a zinc-dependent dehydrogenase with a GroES domain, whose gene is present in genomes in many organisms capable of syntrophy, and transcriptional regulators responsive to environmental stimuli or the physiological status of the cell. The proteomic analysis revealed an emphasis macromolecular stability and energy metabolism to S. wolfei and presence of regulatory mechanisms responsive to external stimuli and cellular physiological status.

  1. Transcriptomic analysis of the oleaginous microalga Neochloris oleoabundans reveals metabolic insights into triacylglyceride accumulation

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    Rismani-Yazdi Hamid

    2012-09-01

    Full Text Available Abstract Background The lack of sequenced genomes for oleaginous microalgae limits our understanding of the mechanisms these organisms utilize to become enriched in triglycerides. Here we report the de novo transcriptome assembly and quantitative gene expression analysis of the oleaginous microalga Neochloris oleoabundans, with a focus on the complex interaction of pathways associated with the production of the triacylglycerol (TAG biofuel precursor. Results After growth under nitrogen replete and nitrogen limiting conditions, we quantified the cellular content of major biomolecules including total lipids, triacylglycerides, starch, protein, and chlorophyll. Transcribed genes were sequenced, the transcriptome was assembled de novo, and the expression of major functional categories, relevant pathways, and important genes was quantified through the mapping of reads to the transcriptome. Over 87 million, 77 base pair high quality reads were produced on the Illumina HiSeq sequencing platform. Metabolite measurements supported by genes and pathway expression results indicated that under the nitrogen-limiting condition, carbon is partitioned toward triglyceride production, which increased fivefold over the nitrogen-replete control. In addition to the observed overexpression of the fatty acid synthesis pathway, TAG production during nitrogen limitation was bolstered by repression of the β-oxidation pathway, up-regulation of genes encoding for the pyruvate dehydrogenase complex which funnels acetyl-CoA to lipid biosynthesis, activation of the pentose phosphate pathway to supply reducing equivalents to inorganic nitrogen assimilation and fatty acid biosynthesis, and the up-regulation of lipases—presumably to reconstruct cell membranes in order to supply additional fatty acids for TAG biosynthesis. Conclusions Our quantitative transcriptome study reveals a broad overview of how nitrogen stress results in excess TAG production in N. oleoabundans, and

  2. Molecular determinants of juvenile hormone action as revealed by 3D QSAR analysis in Drosophila.

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    Denisa Liszeková

    Full Text Available BACKGROUND: Postembryonic development, including metamorphosis, of many animals is under control of hormones. In Drosophila and other insects these developmental transitions are regulated by the coordinate action of two principal hormones, the steroid ecdysone and the sesquiterpenoid juvenile hormone (JH. While the mode of ecdysone action is relatively well understood, the molecular mode of JH action remains elusive. METHODOLOGY/PRINCIPAL FINDINGS: To gain more insights into the molecular mechanism of JH action, we have tested the biological activity of 86 structurally diverse JH agonists in Drosophila melanogaster. The results were evaluated using 3D QSAR analyses involving CoMFA and CoMSIA procedures. Using this approach we have generated both computer-aided and species-specific pharmacophore fingerprints of JH and its agonists, which revealed that the most active compounds must possess an electronegative atom (oxygen or nitrogen at both ends of the molecule. When either of these electronegative atoms are replaced by carbon or the distance between them is shorter than 11.5 A or longer than 13.5 A, their biological activity is dramatically decreased. The presence of an electron-deficient moiety in the middle of the JH agonist is also essential for high activity. CONCLUSIONS/SIGNIFICANCE: The information from 3D QSAR provides guidelines and mechanistic scope for identification of steric and electrostatic properties as well as donor and acceptor hydrogen-bonding that are important features of the ligand-binding cavity of a JH target protein. In order to refine the pharmacophore analysis and evaluate the outcomes of the CoMFA and CoMSIA study we used pseudoreceptor modeling software PrGen to generate a putative binding site surrogate that is composed of eight amino acid residues corresponding to the defined molecular interactions.

  3. Diversity in a Polymicrobial Community Revealed by Analysis of Viromes, Endolysins and CRISPR Spacers

    Science.gov (United States)

    Treangen, Todd J.; Koren, Sergey; Pop, Mihai; Bhaya, Devaki

    2016-01-01

    The polymicrobial biofilm communities in Mushroom and Octopus Spring in Yellowstone National Park (YNP) are well characterized, yet little is known about the phage populations. Dominant species, Synechococcus sp. JA-2-3B'a(2–13), Synechococcus sp. JA-3-3Ab, Chloroflexus sp. Y-400-fl, and Roseiflexus sp. RS-1, contain multiple CRISPR-Cas arrays, suggesting complex interactions with phage predators. To analyze phage populations from Octopus Spring biofilms, we sequenced a viral enriched fraction. To assemble and analyze phage metagenomic data, we developed a custom module, VIRITAS, implemented within the MetAMOS framework. This module bins contigs into groups based on tetranucleotide frequencies and CRISPR spacer-protospacer matching and ORF calling. Using this pipeline we were able to assemble phage sequences into contigs and bin them into three clusters that corroborated with their potential host range. The virome contained 52,348 predicted ORFs; some were clearly phage-like; 9319 ORFs had a recognizable Pfam domain while the rest were hypothetical. Of the recognized domains with CRISPR spacer matches, was the phage endolysin used by lytic phage to disrupt cells. Analysis of the endolysins present in the thermophilic cyanophage contigs revealed a subset of characterized endolysins as well as a Glyco_hydro_108 (PF05838) domain not previously associated with sequenced cyanophages. A search for CRISPR spacer matches to all identified phage endolysins demonstrated that a majority of endolysin domains were targets. This strategy provides a general way to link host and phage as endolysins are known to be widely distributed in bacteriophage. Endolysins can also provide information about host cell wall composition and have the additional potential to be used as targets for novel therapeutics. PMID:27611571

  4. Analysis of newly established EST databases reveals similarities between heart regeneration in newt and fish

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    Weis Patrick

    2010-01-01

    Full Text Available Abstract Background The newt Notophthalmus viridescens possesses the remarkable ability to respond to cardiac damage by formation of new myocardial tissue. Surprisingly little is known about changes in gene activities that occur during the course of regeneration. To begin to decipher the molecular processes, that underlie restoration of functional cardiac tissue, we generated an EST database from regenerating newt hearts and compared the transcriptional profile of selected candidates with genes deregulated during zebrafish heart regeneration. Results A cDNA library of 100,000 cDNA clones was generated from newt hearts 14 days after ventricular injury. Sequencing of 11520 cDNA clones resulted in 2894 assembled contigs. BLAST searches revealed 1695 sequences with potential homology to sequences from the NCBI database. BLAST searches to TrEMBL and Swiss-Prot databases assigned 1116 proteins to Gene Ontology terms. We also identified a relatively large set of 174 ORFs, which are likely to be unique for urodele amphibians. Expression analysis of newt-zebrafish homologues confirmed the deregulation of selected genes during heart regeneration. Sequences, BLAST results and GO annotations were visualized in a relational web based database followed by grouping of identified proteins into clusters of GO Terms. Comparison of data from regenerating zebrafish hearts identified biological processes, which were uniformly overrepresented during cardiac regeneration in newt and zebrafish. Conclusion We concluded that heart regeneration in newts and zebrafish led to the activation of similar sets of genes, which suggests that heart regeneration in both species might follow similar principles. The design of the newly established newt EST database allows identification of molecular pathways important for heart regeneration.

  5. Genome Analysis of Two Pseudonocardia Phylotypes Associated with Acromyrmex Leafcutter Ants Reveals Their Biosynthetic Potential.

    Science.gov (United States)

    Holmes, Neil A; Innocent, Tabitha M; Heine, Daniel; Bassam, Mahmoud Al; Worsley, Sarah F; Trottmann, Felix; Patrick, Elaine H; Yu, Douglas W; Murrell, J C; Schiøtt, Morten; Wilkinson, Barrie; Boomsma, Jacobus J; Hutchings, Matthew I

    2016-01-01

    The attine ants of South and Central America are ancient farmers, having evolved a symbiosis with a fungal food crop >50 million years ago. The most evolutionarily derived attines are the Atta and Acromyrmex leafcutter ants, which harvest fresh leaves to feed their fungus. Acromyrmex and many other attines vertically transmit a mutualistic strain of Pseudonocardia and use antifungal compounds made by these bacteria to protect their fungal partner against co-evolved fungal pathogens of the genus Escovopsis. Pseudonocardia mutualists associated with the attines Apterostigma dentigerum and Trachymyrmex cornetzi make novel cyclic depsipeptide compounds called gerumycins, while a mutualist strain isolated from derived Acromyrmex octospinosus makes an unusual polyene antifungal called nystatin P1. The novelty of these antimicrobials suggests there is merit in exploring secondary metabolites of Pseudonocardia on a genome-wide scale. Here, we report a genomic analysis of the Pseudonocardia phylotypes Ps1 and Ps2 that are consistently associated with Acromyrmex ants collected in Gamboa, Panama. These were previously distinguished solely on the basis of 16S rRNA gene sequencing but genome sequencing of five Ps1 and five Ps2 strains revealed that the phylotypes are distinct species and each encodes between 11 and 15 secondary metabolite biosynthetic gene clusters (BGCs). There are signature BGCs for Ps1 and Ps2 strains and some that are conserved in both. Ps1 strains all contain BGCs encoding nystatin P1-like antifungals, while the Ps2 strains encode novel nystatin-like molecules. Strains show variations in the arrangement of these BGCs that resemble those seen in gerumycin gene clusters. Genome analyses and invasion assays support our hypothesis that vertically transmitted Ps1 and Ps2 strains have antibacterial activity that could help shape the cuticular microbiome. Thus, our work defines the Pseudonocardia species associated with Acromyrmex ants and supports the hypothesis

  6. Particle Motion Analysis Reveals Nanoscale Bond Characteristics and Enhances Dynamic Range for Biosensing.

    Science.gov (United States)

    Visser, Emiel W A; van IJzendoorn, Leo J; Prins, Menno W J

    2016-03-22

    Biofunctionalized colloidal particles are widely used as labels in bioanalytical assays, lab-on-chip devices, biophysical research, and in studies on live biological systems. With detection resolution going down to the level of single particles and single molecules, understanding the nature of the interaction of the particles with surfaces and substrates becomes of paramount importance. Here, we present a comprehensive study of motion patterns of colloidal particles maintained in close proximity to a substrate by short molecular tethers (40 nm). The motion of the particles (500-1000 nm) was optically tracked with a very high localization accuracy (below 3 nm). A surprisingly large variation in motion patterns was observed, which can be attributed to properties of the particle-molecule-substrate system, namely the bond number, the nature of the bond, particle protrusions, and substrate nonuniformities. Experimentally observed motion patterns were compared to numerical Monte Carlo simulations, revealing a close correspondence between the observed motion patterns and properties of the molecular system. Particles bound via single tethers show distinct disc-, ring-, and bell-shaped motion patterns, where the ring- and bell-shaped patterns are caused by protrusions on the particle in the direct vicinity of the molecular attachment point. Double and triple tethered particles exhibit stripe-shaped and triangular-shaped motion patterns, respectively. The developed motion pattern analysis allows for discrimination between particles bound by different bond types, which opens the possibility to improve the limit of detection and the dynamic range of bioanalytical assays, with a projected increase of dynamic range by nearly 2 orders of magnitude.

  7. Evolutionary history of barley cultivation in Europe revealed by genetic analysis of extant landraces

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    Jones Huw

    2011-11-01

    Full Text Available Abstract Background Understanding the evolution of cultivated barley is important for two reasons. First, the evolutionary relationships between different landraces might provide information on the spread and subsequent development of barley cultivation, including the adaptation of the crop to new environments and its response to human selection. Second, evolutionary information would enable landraces with similar traits but different genetic backgrounds to be identified, providing alternative strategies for the introduction of these traits into modern germplasm. Results The evolutionary relationships between 651 barley landraces were inferred from the genotypes for 24 microsatellites. The landraces could be divided into nine populations, each with a different geographical distribution. Comparisons with ear row number, caryopsis structure, seasonal growth habit and flowering time revealed a degree of association between population structure and phenotype, and analysis of climate variables indicated that the landraces are adapted, at least to some extent, to their environment. Human selection and/or environmental adaptation may therefore have played a role in the origin and/or maintenance of one or more of the barley landrace populations. There was also evidence that at least some of the population structure derived from geographical partitioning set up during the initial spread of barley cultivation into Europe, or reflected the later introduction of novel varieties. In particular, three closely-related populations were made up almost entirely of plants with the daylength nonresponsive version of the photoperiod response gene PPD-H1, conferring adaptation to the long annual growth season of northern Europe. These three populations probably originated in the eastern Fertile Crescent and entered Europe after the initial spread of agriculture. Conclusions The discovery of population structure, combined with knowledge of associated phenotypes and

  8. Transcriptome Analysis of Chilling-Imbibed Embryo Revealed Membrane Recovery Related Genes in Maize

    Science.gov (United States)

    He, Fei; Shen, Hangqi; Lin, Cheng; Fu, Hong; Sheteiwy, Mohamed S.; Guan, Yajing; Huang, Yutao; Hu, Jin

    2017-01-01

    The delayed seed germination and poor seedling growth caused by imbibitional chilling injury was common phenomenon in maize seedling establishment. In this study, RNA sequencing technology was used to comprehensively investigate the gene expressions in chilling-imbibed maize embryo and to reveal the underlying mechanism of chilling injury at molecular level. Imbibed seeds for 2 h at 5°C (LT2) were selected and transcriptomic comparative analysis was performed. Among 327 DEGs indentified between dry seed (CK0) and LT2, 15 specific genes with plasma membrane (PM) relevant functions belonging to lipid metabolism, stress, signaling and transport were characterized, and most of them showed down-regulation pattern under chilling stress. When transferred to 25°C for recovery (LT3), remarkable changes occurred in maize embryo. There were 873 DEGs including many PM related genes being identified between LT2 and LT3, some of which showing significant increase after 1 h recovery. Moreover, 15 genes encoding intracellular vesicular trafficking proteins were found to be exclusively differential expressed at recovery stage. It suggested that the intracellular vesicle trafficking might be essential for PM recovery through PM turnover. Furthermore, transcriptome analyses on imbibed embryos under normal condition (25°C) were also made as a contrast. A total of 651 DEGs were identified to mainly involved in protein metabolism, transcriptional regulation, signaling, and energy productions. Overall, the RNA-Seq results provided us a deep knowledge of imbibitional chilling injury on plasma membrane and a new view on PM repaired mechanism during early seed imbibition at transcriptional level. The DEGs identified in this work would be useful references in future seed germination research. PMID:28101090

  9. Optimality Functions and Lopsided Convergence

    Science.gov (United States)

    2015-03-16

    Problems involving functions defined in terms of integrals or optimization problems (as the maxi - mization in Example 3), functions defined on infinite...optimization methods in finite time. The key technical challenge associate with the above scheme is to establish ( weak ) consistency. In the next...Theorem 4.3. In view of this result, it is clear that ( weak ) consistency will be ensured by epi-convergence of the approximating objective functions and

  10. ACCOUNTING CONVERGENCE ON ECONOMIC CRISIS

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    HOLBAN ONCIOIU IONICA

    2010-12-01

    Full Text Available If normalization accounting is the process of harmonizing the presentation of financial statements, accounting methods and terminology, accounting convergence is the process by which accounting standards are developed in a way that is able to lead to the same fact or purpose, by highlighting the similarity of national - regional - international. This paper put in light the importance of the normalization and harmonization process, what its done in and what we have to do for that in Romania.

  11. Surface meshing with curvature convergence

    KAUST Repository

    Li, Huibin

    2014-06-01

    Surface meshing plays a fundamental role in graphics and visualization. Many geometric processing tasks involve solving geometric PDEs on meshes. The numerical stability, convergence rates and approximation errors are largely determined by the mesh qualities. In practice, Delaunay refinement algorithms offer satisfactory solutions to high quality mesh generations. The theoretical proofs for volume based and surface based Delaunay refinement algorithms have been established, but those for conformal parameterization based ones remain wide open. This work focuses on the curvature measure convergence for the conformal parameterization based Delaunay refinement algorithms. Given a metric surface, the proposed approach triangulates its conformal uniformization domain by the planar Delaunay refinement algorithms, and produces a high quality mesh. We give explicit estimates for the Hausdorff distance, the normal deviation, and the differences in curvature measures between the surface and the mesh. In contrast to the conventional results based on volumetric Delaunay refinement, our stronger estimates are independent of the mesh structure and directly guarantee the convergence of curvature measures. Meanwhile, our result on Gaussian curvature measure is intrinsic to the Riemannian metric and independent of the embedding. In practice, our meshing algorithm is much easier to implement and much more efficient. The experimental results verified our theoretical results and demonstrated the efficiency of the meshing algorithm. © 2014 IEEE.

  12. Convergences and divergences in mortality

    Directory of Open Access Journals (Sweden)

    2004-04-01

    Full Text Available Abdel Omran's 1971 theory of "Epidemiologic Transition" was the first attempt to account for the extraordinary advances in health care made in industrialized countries since the 18th century. In the framework of the Demographic Transition, it implied a general convergence of life expectancies toward a limit imposed by the new epidemiological features of modern societies. However, important failures, occurred in the past decades (mainly the health crisis in Eastern Europe and AIDS in Africa, seem to have stopped that process of convergence. In fact such failures do not really contradict the theory. The latter is much more ruined by the unexpected dramatic improvement in the field of cardiovascular disease experienced since the seventies, which results in a new step of a more general process. On the basis of the broader concept of "Health Transition" initiated by Julio Frenk et al., the present paper tries to rethink the full process in term of divergence/convergence sequences inferred by successive major changes in health technologies and strategies.

  13. User Configurations and Convergence in Consumer Electronics

    DEFF Research Database (Denmark)

    Jørgensen, Ulrik

    2000-01-01

    Convergence has been a technical fix motivated by the digital developments in media technologies.......Convergence has been a technical fix motivated by the digital developments in media technologies....

  14. CONVERGENCE THEOREMS FOR THE AOR METHOD

    Institute of Scientific and Technical Information of China (English)

    黄廷祝; 王广彬

    2002-01-01

    Practical sufficient conditions for the convergence of the AOR method and a practical sufficient condition for H-matrices are studied. The obtained convergence conditions suited to matrices which need not to be diagonally dominant.

  15. 子结构快速多极子边界元法声学迭代计算收敛特性分析%Convergence property analysis for acoustic iterative calculation of substructure FMBEM

    Institute of Scientific and Technical Information of China (English)

    崔晓兵

    2015-01-01

    Substructure FMBEM is developed by the application of substructure technique into Fast Multipole Boundary Element Method (FMBEM). It carries out the investigation and analysis of its iterative convergence situation and the inlfuence factors by complex structure acoustic ifeld calculation. It is found that the building of matrix equation, scale of interface and filling of the sound-absorbing material have significant influence on convergence speed when the problem is solved by the programs with FORTRAN language. Finally, the transmission loss of a silencer is calculated by the substructure FMBEM with comparison with conventional BEM in order to conifrm its correctness and accuracy.%将子结构技术应用到快速多极子边界元法中,形成子结构快速多极子边界元法。通过复杂结构声场计算,对该方法的迭代计算收敛情况以及影响收敛的因素进行研究分析。经研究发现,应用Fortran语言编程求解问题时,矩阵方程构建方式,交界面的规模以及是否填充吸声材料对迭代法的收敛速度有着重要影响。最后,以应用子结构快速多极子边界元法与传统边界元法计算消声器传递损失为例,对该方法的准确性和精度进行验证。

  16. 媒介融合背景下版权机制存在的问题及影响因素分析%Analysis on the Problems and Influencing Factors of Copyright Mechanism under the Background of Media Convergence

    Institute of Scientific and Technical Information of China (English)

    刘玲武; 黄先蓉

    2016-01-01

    我国版权机制经过多年的发展取得显著的成就。媒介融合背景下,由于版权主体增多、版权客体扩充、权利内容扩张、版权法律滞后、版权市场无序等带来的版权授权模式迟滞、交易平台作用有限、保护机制疲软等问题,严重阻碍版权机制的发展,难以平衡权利人、传播者和社会公众的利益。本文重点分析媒介融合背景下影响版权机制的因素及其具体表现,为今后制定版权机制发展策略提供参考。%After the years of development, China' s copyright mechanism has made remarkable achievements. Under the background of media convergence, the license pat ern lags behind the needs mass authorization of copyright, the trading platforms of copyright have limitations and the copyright protection mechanism is weak due to copyright subject increasing, copyright object expansion, copyright content expansion, lag of copyright law and disorder of copyright market. These problems seriously impede the development of copyright mechanism and also difficult to balance the interests among the authors, communicators and the public. The paper focuses on the analysis of the factors and specific performance which influence copyright mechanism under the background of media convergence, in order to provide a reference for the future development of copyright mechanism.

  17. Adapting Predictive Feedback Chaos Control for Optimal Convergence Speed

    CERN Document Server

    Bick, Christian; Kolodziejski, Christoph

    2012-01-01

    Stabilizing unstable periodic orbits in a chaotic invariant set not only reveals information about its structure but also leads to various interesting applications. For the successful application of a chaos control scheme, convergence speed is of crucial importance. Here we present a predictive feedback chaos control method that adapts a control parameter online to yield optimal asymptotic convergence speed. We study the adaptive control map both analytically and numerically and prove that it converges at least linearly to a value determined by the spectral radius of the control map at the periodic orbit to be stabilized. The method is easy to implement algorithmically and may find applications for adaptive online control of biological and engineering systems.

  18. Analysis of global gene expression in Brachypodium distachyon reveals extensive network plasticity in response to abiotic stress.

    Directory of Open Access Journals (Sweden)

    Henry D Priest

    Full Text Available Brachypodium distachyon is a close relative of many important cereal crops. Abiotic stress tolerance has a significant impact on productivity of agriculturally important food and feedstock crops. Analysis of the transcriptome of Brachypodium after chilling, high-salinity, drought, and heat stresses revealed diverse differential expression of many transcripts. Weighted Gene Co-Expression Network Analysis revealed 22 distinct gene modules with specific profiles of expression under each stress. Promoter analysis implicated short DNA sequences directly upstream of module members in the regulation of 21 of 22 modules. Functional analysis of module members revealed enrichment in functional terms for 10 of 22 network modules. Analysis of condition-specific correlations between differentially expressed gene pairs revealed extensive plasticity in the expression relationships of gene pairs. Photosynthesis, cell cycle, and cell wall expression modules were down-regulated by all abiotic stresses. Modules which were up-regulated by each abiotic stress fell into diverse and unique gene ontology GO categories. This study provides genomics resources and improves our understanding of abiotic stress responses of Brachypodium.

  19. Neuroanatomical correlates of tinnitus revealed by cortical thickness analysis and diffusion tensor imaging

    Energy Technology Data Exchange (ETDEWEB)

    Aldhafeeri, Faten M. [The University of Liverpool, Department of Medical Imaging, School of Health Sciences, Liverpool (United Kingdom); King Khalid General Hospital, Ministry of Health, Radiology Department, Hafral-batin (Saudi Arabia); Mackenzie, Ian; Kay, Tony [Aintree University Hospitals NHS Foundation Trust, Liverpool (United Kingdom); Alghamdi, Jamaan [The University of Liverpool, Department of Medical Imaging, School of Health Sciences, Liverpool (United Kingdom); King Abdul Aziz University, Physics Department, Faculty of Sciences, Jeddah (Saudi Arabia); Sluming, Vanessa [The University of Liverpool, Department of Medical Imaging, School of Health Sciences, Liverpool (United Kingdom); Magnetic Resonance and Image Analysis Research Centre, Liverpool (United Kingdom)

    2012-08-15

    Tinnitus is a poorly understood auditory perception of sound in the absence of external stimuli. Convergent evidence proposes that tinnitus perception involves brain structural alterations as part of its pathophysiology. The aim of this study is to investigate the structural brain changes that might be associated with tinnitus-related stress and negative emotions. Using high-resolution magnetic resonance imaging and diffusion tensor imaging, we investigated grey matter and white matter (WM) alterations by estimating cortical thickness measures, fractional anisotropy and mean diffusivity in 14 tinnitus subjects and 14 age- and sex-matched non-tinnitus subjects. Significant cortical thickness reductions were found in the prefrontal cortex (PFC), temporal lobe and limbic system in tinnitus subjects compared to non-tinnitus subjects. Tinnitus sufferers were found to have disrupted WM integrity in tracts involving connectivity of the PFC, temporal lobe, thalamus and limbic system. Our results suggest that such neural changes may represent neural origins for tinnitus or consequences of tinnitus and its associations. (orig.)

  20. Variable Number Tandem Repeat (VNTR) analysis reveals genetic diversity within Mycoplasma mycoides mycoides small colony isolates from Nigeria.

    Science.gov (United States)

    Nwankpa, N D; Manso-silvan, L; Lorenzon, S; Yaya, A; Lombin, L H; Thiaucourt, F

    2010-12-15

    A Variable Number Tandem Repeat (VNTR) analysis was conducted on thirteen (13) M. mycoides mycoides Small Colony isolates from Nigeria using Tandem Repeat (TR) 34 which is a predicted lipoprotein located within the hypothetical protein MAG6170. The analysis revealed diversity within the M. mycoides mycoides Small Colony isolates with five different VNTR types indicated. Some correlation was determined between the VNTR types and their geographical origin. VNTR analysis may represent a useful, rapid first-line test for use in molecular epidemiological analysis of M. mycoides mycoides Small Colony for possible outbreak tracing and disease control.

  1. TAUBERIAN THEOREMS FOR WEAK ALMOST CONVERGENT FUNCTIONS

    Institute of Scientific and Technical Information of China (English)

    Meng-Kuang Kuo

    2011-01-01

    The almost convergent function which was introduced by Raimi [6] and dis- cussed by Ho [4], Das and Nanda [2, 3], is the continuous analogue of almost convergent sequences (see [5]). In this paper, we establish the Tauberian conditions and the Cauchy criteria for weak almost convergent functions on R2+.

  2. International Convergence on Geoscience Cyberinfrastructure

    Science.gov (United States)

    Allison, M. L.; Atkinson, R.; Arctur, D. K.; Cox, S.; Jackson, I.; Nativi, S.; Wyborn, L. A.

    2012-04-01

    There is growing international consensus on addressing the challenges to cyber(e)-infrastructure for the geosciences. These challenges include: Creating common standards and protocols; Engaging the vast number of distributed data resources; Establishing practices for recognition of and respect for intellectual property; Developing simple data and resource discovery and access systems; Building mechanisms to encourage development of web service tools and workflows for data analysis; Brokering the diverse disciplinary service buses; Creating sustainable business models for maintenance and evolution of information resources; Integrating the data management life-cycle into the practice of science. Efforts around the world are converging towards de facto creation of an integrated global digital data network for the geosciences based on common standards and protocols for data discovery and access, and a shared vision of distributed, web-based, open source interoperable data access and integration. Commonalities include use of Open Geospatial Consortium (OGC) and ISO specifications and standardized data interchange mechanisms. For multidisciplinarity, mediation, adaptation, and profiling services have been successfully introduced to leverage the geosciences standards which are commonly used by the different geoscience communities -introducing a brokering approach which extends the basic SOA archetype. Principal challenges are less technical than cultural, social, and organizational. Before we can make data interoperable, we must make people interoperable. These challenges are being met by increased coordination of development activities (technical, organizational, social) among leaders and practitioners in national and international efforts across the geosciences to foster commonalities across disparate networks. In doing so, we will 1) leverage and share resources, and developments, 2) facilitate and enhance emerging technical and structural advances, 3) promote

  3. Convergence of a Stochastic Method for the Modeling of Polymeric Fluids

    Institute of Scientific and Technical Information of China (English)

    Weinan E; Tie-jun Li; Ping-wen Zhang

    2002-01-01

    We present a convergence analysis of a stochastic method for numerical modeling of complex fluids using Brownian configuration fields (BCF) for shear flows. The analysis takes into account the special structure of the stochastic partial differential equations for shear flows. We establish the optimal rate of convergence. We also analyze the nature of the error by providing its leading order asymptotics.

  4. Weak* convergence of operator means

    Energy Technology Data Exchange (ETDEWEB)

    Romanov, Alexandr V [Moscow State Institute of Electronics and Mathematics (Technical University), Moscow (Russian Federation)

    2011-12-31

    For a linear operator U with ||U'n||{<=}const on a Banach space X we discuss conditions for the convergence of ergodic operator nets T{sub {alpha}} corresponding to the adjoint operator U* of U in the W*O-topology of the space EndX*. The accumulation points of all possible nets of this kind form a compact convex set L in EndX*, which is the kernel of the operator, where {Gamma}{sub 0}={l_brace}U{sub n}*, n{>=}0{r_brace}. It is proved that all ergodic nets T{sub {alpha}} weakly* converge if and only if the kernel L consists of a single element. In the case of X=C({Omega}) and the shift operator U generated by a continuous transformation {phi} of a metrizable compactum {Omega} we trace the relationships among the ergodic properties of U, the structure of the operator semigroups L, G and {Gamma}={Gamma}-bar{sub 0}, and the dynamical characteristics of the semi-cascade ({phi},{Omega}). In particular, if cardL=1, then a) for any {omega} element of {Omega} the closure of the trajectory {l_brace}{phi}{sup n}{omega}, n{>=}0{r_brace} contains precisely one minimal set m, and b) the restriction ({phi},m) is strictly ergodic. Condition a) implies the W*O-convergence of any ergodic sequence of operators T{sub n} element of EndX* under the additional assumption that the kernel of the enveloping semigroup E({phi},{Omega}) contains elements obtained from the 'basis' family of transformations {l_brace}{phi}{sup n}, n{>=}0{r_brace} of the compact set {Omega} by using some transfinite sequence of sequential passages to the limit.

  5. Ecomorphological convergence in planktivorous surgeonfishes

    KAUST Repository

    Friedman, S. T.

    2016-01-26

    © 2016 European Society For Evolutionary Biology. Morphological convergence plays a central role in the study of evolution. Often induced by shared ecological specialization, homoplasy hints at underlying selective pressures and adaptive constraints that deterministically shape the diversification of life. Although midwater zooplanktivory has arisen in adult surgeonfishes (family Acanthuridae) at least four independent times, it represents a clearly specialized state, requiring the capacity to swiftly swim in midwater locating and sucking small prey items. Whereas this diet has commonly been associated with specific functional adaptations in fishes, acanthurids present an interesting case study as all nonplanktivorous species feed by grazing on benthic algae and detritus, requiring a vastly different functional morphology that emphasizes biting behaviours. We examined the feeding morphology in 30 acanthurid species and, combined with a pre-existing phylogenetic tree, compared the fit of evolutionary models across two diet regimes: zooplanktivores and nonzooplanktivorous grazers. Accounting for phylogenetic relationships, the best-fitting model indicates that zooplanktivorous species are converging on a separate adaptive peak from their grazing relatives. Driving this bimodal landscape, zooplanktivorous acanthurids tend to develop a slender body, reduced facial features, smaller teeth and weakened jaw adductor muscles. However, despite these phenotypic changes, model fitting suggests that lineages have not yet reached the adaptive peak associated with plankton feeding even though some transitions appear to be over 10 million years old. These findings demonstrate that the selective demands of pelagic feeding promote repeated - albeit very gradual - ecomorphological convergence within surgeonfishes, while allowing local divergences between closely related species, contributing to the overall diversity of the clade. Journal of Evolutionary Biology

  6. SCORE DIGITAL TECHNOLOGY: THE CONVERGENCE

    Directory of Open Access Journals (Sweden)

    Chernyshov Alexander V.

    2013-12-01

    Full Text Available Explores the role of digital scorewriters in today's culture, education, and music industry and media environment. The main principle of the development of software is not only publishing innovation (relating to the sheet music, and integration into the area of composition, arrangement, education, creative process for works based on digital technology (films, television and radio broadcasting, Internet, audio and video art. Therefore the own convergence of musically-computer technology is a total phenomenon: notation program combined with means MIDI-sequencer, audio and video editor. The article contains the unique interview with the creator of music notation processors.

  7. Convergence of some leader election algorithms

    CERN Document Server

    Janson, Svante; Louchard, Guy

    2008-01-01

    We start with a set of n players. With some probability P(n,k), we kill n-k players; the other ones stay alive, and we repeat with them. What is the distribution of the number X_n of phases (or rounds) before getting only one player? We present a probabilistic analysis of this algorithm under some conditions on the probability distributions P(n,k), including stochastic monotonicity and the assumption that roughly a fixed proportion alpha of the players survive in each round. We prove a kind of convergence in distribution for X_n-log_a n, where the basis a=1/alpha; as in many other similar problems there are oscillations and no true limit distribution, but suitable subsequences converge, and there is an absolutely continuous random variable Z such that the distribution of X_n can be approximated by Z+log_a n rounded to the nearest larger integer. Applications of the general result include the leader election algorithm where players are eliminated by independent coin tosses and a variation of the leader electio...

  8. Convergence of some leader election algorithms

    Directory of Open Access Journals (Sweden)

    Svante Janson

    2008-08-01

    Full Text Available We start with a set of n players. With some probability P(n,k, we kill n-k players; the other ones stay alive, and we repeat with them. What is the distribution of the number X n of phases (or rounds before getting only one player? We present a probabilistic analysis of this algorithm under some conditions on the probability distributions P(n,k, including stochastic monotonicity and the assumption that roughly a fixed proportion α of the players survive in each round. We prove a kind of convergence in distribution for X n - log 1/α n; as in many other similar problems there are oscillations and no true limit distribution, but suitable subsequences converge, and there is an absolutely continuous random variable Z such that d(X n, ⌈Z+ log 1/α n⌉→0, where d is either the total variation distance or the Wasserstein distance. Applications of the general result include the leader election algorithm where players are eliminated by independent coin tosses and a variation of the leader election algorithm proposed by W.R. Franklin 1982. We study the latter algorithm further, including numerical results.

  9. Phylogeographic analysis reveals significant spatial genetic structure of Incarvillea sinensis as a product of mountain building

    Directory of Open Access Journals (Sweden)

    Chen Shaotian

    2012-04-01

    Full Text Available Abstract Background Incarvillea sinensis is widely distributed from Southwest China to Northeast China and in the Russian Far East. The distribution of this species was thought to be influenced by the uplift of the Qinghai-Tibet Plateau and Quaternary glaciation. To reveal the imprints of geological events on the spatial genetic structure of Incarvillea sinensis, we examined two cpDNA segments ( trnH- psbA and trnS- trnfM in 705 individuals from 47 localities. Results A total of 16 haplotypes was identified, and significant genetic differentiation was revealed (GST =0.843, NST = 0.975, P  Conclusions The results revealed that the uplift of the Qinghai-Tibet Plateau likely resulted in the significant divergence between the lineage in the eastern Qinghai-Tibet Plateau and the other one outside this area. The diverse niches in the eastern Qinghai-Tibet Plateau created a wide spectrum of habitats to accumulate and accommodate new mutations. The features of genetic diversity of populations outside the eastern Qinghai-Tibet Plateau seemed to reveal the imprints of extinction during the Glacial and the interglacial and postglacial recolonization. Our study is a typical case of the significance of the uplift of the Qinghai-Tibet Plateau and the Quaternary Glacial in spatial genetic structure of eastern Asian plants, and sheds new light on the evolution of biodiversity in the Qinghai-Tibet Plateau at the intraspecies level.

  10. The Effect of IFRS Convergence and the Proportion of Woman in Audit Committee on Earning Management

    OpenAIRE

    Siswanti Dwi Surti; Hudayati Ataina

    2017-01-01

    This study aims to find empirical evidence the effect of IFRS convergence and the proportion of woman in audit committee on earning management. Respondents used in this study were companies listed in Jakarta Stock Exchange for the year 2011 until 2013. Using moderated regression analysis this study supports the hypothesis that IFRS convergence and the proportion of woman in audit committee have negative effect on earning management. This finding implies that IFRS convergence and the proportio...

  11. Relatório Modificado de auditoria: análise pré e pós-convergência aos padrões internacionais de contabilidade = Audit Modified Report: analysis pre and post-convergence to internacional accounting standards

    Directory of Open Access Journals (Sweden)

    Adalene Olivia Silvestre

    2016-04-01

    Full Text Available Em uma realidade negocial internacionalizada, a divulgação de informações adequadas, transparentes e comparáveis se tornou essencial, concedendo grande importância às normas internacionais de contabilidade. A realização de auditoria independente e a consequente emissão de seu relatório conferem às demonstrações contábeis maior credibilidade. Assim, o presente estudo tem como objetivo verificar quais são os principais motivos que ocasionam a emissão de relatório de auditoria independente (RAI modificado, nas empresas listadas no segmento tradicional da BM&FBOVESPA, no período pré e pós-convergência aos padrões internacionais de contabilidade. Para atender ao objetivo, analisou-se os RAI emitidos entre os anos de 2004 a 2007, período pré-convergência, e os anos de 2010 a 2013, período pós-convergência. Foram coletados os RAI das 250 empresas listadas no segmento tradicional da BM&FBOVESPA, das quais 184 apresentaram os RAI referentes a todos os exercícios, foco do estudo, totalizando 1.472 relatórios. Do total dos RAI analisados 206 apresentaram modificações. Sobre esses, realizou-se análise dos motivos que justificaram a modificação e buscou-se a relação existente entre os motivadores do período pós-convergência e as normas internacionais. Os principais resultados demonstram que os motivos predominantes no período pré-convergência são investimentos e tributos; já no período pós-convergência são investimentos e continuidade. Quanto à relação entre os motivadores do período pós-convergência e as alterações trazidas pelas normas internacionais, verificou-se que do total de 213 motivadores, 36 referem-se às alterações, principalmente devido a teste de impairment, arrendamento mercantil, intangível e empréstimos e financiamentos.In an internationalized negotiating reality, the disclosure of suitable, transparent and comparable information has become essential, providing great importance to

  12. Force-clamp analysis techniques reveal stretched exponential unfolding kinetics in ubiquitin

    CERN Document Server

    Lannon, Herbert; Brujic, Jasna

    2012-01-01

    Force-clamp spectroscopy reveals the unfolding and disulfide bond rupture times of single protein molecules as a function of the stretching force, point mutations and solvent conditions. The statistics of these times reveal whether the protein domains are independent of one another, the mechanical hierarchy in the polyprotein chain, and the functional form of the probability distribution from which they originate. It is therefore important to use robust statistical tests to decipher the correct theoretical model underlying the process. Here we develop multiple techniques to compare the well-established experimental data set on ubiquitin with existing theoretical models as a case study. We show that robustness against filtering, agreement with a maximum likelihood function that takes into account experimental artifacts, the Kuiper statistic test and alignment with synthetic data all identify the Weibull or stretched exponential distribution as the best fitting model. Our results are inconsistent with recently ...

  13. Metagenomic analysis reveals that modern microbialites and polar microbial mats have similar taxonomic and functional potential

    OpenAIRE

    White, Richard Allen; Power, Ian M.; Dipple, Gregory M.; Southam, Gordon; Suttle, Curtis A.

    2015-01-01

    Within the subarctic climate of Clinton Creek, Yukon, Canada, lies an abandoned and flooded open-pit asbestos mine that harbors rapidly growing microbialites. To understand their formation we completed a metagenomic community profile of the microbialites and their surrounding sediments. Assembled metagenomic data revealed that bacteria within the phylum Proteobacteria numerically dominated this system, although the relative abundances of taxa within the phylum varied among environments. Bacte...

  14. Metagenomic analysis reveals that modern microbialites and polar microbial mats have similar taxonomic and functional potential

    OpenAIRE

    III, Richard Allen White; Ian Malcolm Power; Dipple, Gregory M.; Gordon eSoutham; Suttle, Curtis A.

    2015-01-01

    Within the subarctic climate of Clinton Creek, Yukon, Canada, lies an abandoned and flooded open-pit asbestos mine that harbors rapidly growing microbialites. To understand their formation we completed a metagenomic community profile of the microbialites and their surrounding sediments. Assembled metagenomic data revealed that bacteria within the phylum Proteobacteria numerically dominated this system, although the relative abundances of taxa within the phylum varied among environments. Bact...

  15. Genome-wide location analysis reveals a role for Sub1 in RNA polymerase III transcription

    Science.gov (United States)

    Tavenet, Arounie; Suleau, Audrey; Dubreuil, Géraldine; Ferrari, Roberto; Ducrot, Cécile; Michaut, Magali; Aude, Jean-Christophe; Dieci, Giorgio; Lefebvre, Olivier; Conesa, Christine; Acker, Joël

    2009-01-01

    Human PC4 and the yeast ortholog Sub1 have multiple functions in RNA polymerase II transcription. Genome-wide mapping revealed that Sub1 is present on Pol III-transcribed genes. Sub1 was found to interact with components of the Pol III transcription system and to stimulate the initiation and reinitiation steps in a system reconstituted with all recombinant factors. Sub1 was required for optimal Pol III gene transcription in exponentially growing cells. PMID:19706510

  16. Transcriptome analysis in tardigrade species reveals specific molecular pathways for stress adaptations

    OpenAIRE

    Frank Förster; Daniela Beisser; Grohme, Markus A.; Chunguang Liang; Brahim Mali; Alexander Matthias Siegl; Engelmann, Julia C.; Shkumatov, Alexander V; Elham Schokraie; Tobias Müller; Martina Schnölzer; Schill, Ralph O.; Marcus Frohme; Thomas Dandekar

    2012-01-01

    Tardigrades have unique stress-adaptations that allow them to survive extremes of cold, heat, radiation and vacuum. To study this, encoded protein clusters and pathways from an ongoing transcriptome study on the tardigrade \\(Milnesium\\) \\(tardigradum\\) were analyzed using bioinformatics tools and compared to expressed sequence tags (ESTs) from \\(Hypsibius\\) \\(dujardini\\), revealing major pathways involved in resistance against extreme environmental conditions. ESTs are available on the Tardig...

  17. Quantitative analysis of proteome and lipidome dynamics reveals functional regulation of global lipid metabolism

    DEFF Research Database (Denmark)

    Casanovas, Albert; Sprenger, Richard R; Tarasov, Kirill

    2015-01-01

    architecture and processes during physiological adaptations in yeast. Our results reveal that activation of cardiolipin synthesis and remodeling supports mitochondrial biogenesis in the transition from fermentative to respiratory metabolism, that down-regulation of de novo sterol synthesis machinery prompts......Elucidating how and to what extent lipid metabolism is remodeled under changing conditions is essential for understanding cellular physiology. Here, we analyzed proteome and lipidome dynamics to investigate how regulation of lipid metabolism at the global scale supports remodeling of cellular...

  18. Multilocus analysis reveals large genetic diversity in Kluyveromyces marxianus strains isolated from Parmigiano Reggiano and Pecorino di Farindola cheeses.

    Science.gov (United States)

    Fasoli, Giuseppe; Barrio, Eladio; Tofalo, Rosanna; Suzzi, Giovanna; Belloch, Carmela

    2016-09-16

    In the present study, we have analysed the genetic diversity in Kluyveromyces marxianus isolated from Parmigiano Reggiano and Pecorino di Farindola cheesemaking environment. Molecular typing methods inter-RTL fingerprint and mtDNA RFLPs, as well as, sequence diversity and heterozygosity in the intergenic region between KmSSB1 and KmRIO2 genes and analysis of the mating locus were applied to 54 K. marxianus strains. Inter-RTL fingerprint revealed a large degree of genetic heterogeneity and clustering allowed differentiation of K. marxianus strains from different geographical origins. In general, inter-LTR profiles were more discriminating than RFLPs of mtDNA; however our results also indicate that both techniques could be complementary unveiling different degrees of genetic diversity. Sequence analysis of the intergenic region between KmSSB1 and KmRIO2 genes revealed 26 variable positions in which a double peak could be observed in the sequence chromatogram. Further analysis revealed the presence of heterozygous strains in the K. marxianus population isolated from Parmigiano Reggiano. On the other hand, all strains isolated from Pecorino di Farindola were homozygous. Two very different groups of haplotypes could be observed as well as mixtures between them. Phylogenetic reconstruction divided K. marxianus dairy strains into two separate populations. A few heterozygous strains in an intermediate position between them could also be observed. Mating type locus analysis revealed a large population of diploid strains containing both MATa and MATα alleles and few haploid strains, most of them presenting the MATα allele. Different scenarios explaining the presence and maintaining of homozygous and heterozygous diploids as well as hybrids between them in the Parmigiano Reggiano K. marxianus population are proposed. A principal component analysis supported the large differences between K. marxianus isolated from Parmigiano Reggiano and Pecorino di Farindola.

  19. Functional convergence in bat and toothed whale biosonars

    DEFF Research Database (Denmark)

    Madsen, P T; Surlykke, A

    2013-01-01

    Echolocating bats and toothed whales hunt and navigate by emission of sound pulses and analysis of returning echoes to form a self-generated auditory scene. Here, we demonstrate a striking functional convergence in the way these two groups of mammals independently evolved the capability to sense ...

  20. Tourism English Teaching Techniques Converged from Two Different Angles.

    Science.gov (United States)

    Seong, Myeong-Hee

    2001-01-01

    Provides techniques converged from two different angles (learners and tourism English features) for effective tourism English teaching in a junior college in Korea. Used a questionnaire, needs analysis, an instrument for measuring learners' strategies for oral communication, a small-scale classroom study for learners' preferred teaching…