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Sample records for analyse par activation

  1. Examples of analysis by activation; Exemples d'analyse par activation

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    Leveque, P [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

    1955-07-01

    We were used various nuclear reactions to do some analysis by neutron or by X-ray activation. We used the french reactor core Zoe as neutrons sources and an Allis-Chalmers betatron as X-rays sources for the dosage of the light elements. The described processes were revealed fast and particularly useful for determination of traces. The fact that most of them don't require any chemical operations, is especially substantial when the solubilization of the sample is difficult. (M.B.) [French] Nous avons utilise des reactions nucleaires diverses pour effectuer des analyses par activation neutronique ou par rayon X. Nous avons utilise la pile francaise Zoe comme sources de neutrons et un betatron Allis-Chalmers comme sources de rayons X pour le dosage des elements legers. Les procedes decrits se sont reveles rapides et particulierement utiles dans la determination des traces. Le fait que, pour la plupart, ils n'exigent pas d'operations chimiques, est particulierement appreciable lorsque la solubilisation des echantillons est difficile. (M.B.)

  2. Activation analysis using {gamma} photons; Analyse par activation aux photons {gamma}

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    Engelmann, Ch [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

    1967-07-01

    This report summarizes all the data required for using photonuclear reactions in the field of analysis. After a brief review of the elementary properties of nuclear reactions induced by photon irradiation, the main characteristics are given of high energy (E > 20 MeV) Bremsstrahlung sources. The principle of activation analysis based on the use of photons is given. Actual examples of the analytic possibilities are described in detail, in particular in the case of the determination of very small quantities (< 10{sup -6}) of C, N, O and F. The influence of interfering nuclear reactions is discussed. (author) [French] Ce rapport se propose de resumer l'ensemble des connaissances indispensables pour l'utilisation des reactions photonucleaires a des fins analytiques. Apres quelques rappels concernant les proprietes elementaires des reactions nucleaires induites par irradiation dans les photons, les principales caracteristiques des sources de rayonnement de freinage de haute energie (E > 20 MeV)| sont donnees. Le principe de l'analyse par activation aux photons est rappele. Des exemples concrets sur les possibilites analytiques sont developpes, particulierement en ce qui concerne la determination de quantites tres faibles (< 10{sup -6}) de C, N, O et F. L'influence des reactions nucleaires parasites est discutee. (auteur)

  3. Use of gamma spectroscopy in activation analysis; Utilisation de la spectrographie gamma dans l'analyse par activation

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    Leveque, [Commissariat a l' Energie Atomique, Saclay (France).Centre d' Etudes Nucleaires

    1959-07-01

    Brief review of the principles of activation analysis: calculation of activities, decay curves, {beta} absorption curves, examples of application. - Principle and description of the {gamma} spectrograph. - Practical utilisation of the {gamma} spectrograph: analysis by activation, analysis by {beta} - x fluorescence. - Sensitivity limit of the method and precision of the measurements. - Possible improvements to the method: {gamma} spectroscopy with elimination of the Compton effect. (author) [French] Bref rappel des principes de l'analyse par activation: calcul des activites, courbes de decroissance, courbes d'absorption {beta}, exemples d'utilisation. - Principe et description du spectrographe {gamma}. - Utilisation pratique de la spectrographie {gamma}: analyse par activation, analyse par fluorescence {beta} - x. - Limite de sensibilite de la methode et precision des mesures. - Ameliorations possibles de la methode: spectrographe {gamma} avec elimination de l'effet Compton. (auteur)

  4. L'analyse par activation de neutrons de réacteur

    Science.gov (United States)

    Meyer, G.

    2003-02-01

    Quand les neutrons traversent la matière, certains sont transmis sans interaction, les autres interagissent avec le milieu traversé par diffusion et par absorption. Ce phénomène d'absorption est utilisé pour se protéger des neutrons, mais aussi pour les détecter; il peut également être utilisé pour identifier les noyaux “absorbants" et ainsi analyser le milieu traversé. En effet par différentes réactions nucléaires (n,γ), (n,p), (n,α), (n,fission), on obtient des noyaux résiduels qui sont souvent radioactifs; on dit que l'échantillon est “activé". Si l'on connaît le rendement d'activation et donc le pourcentage de noyaux ainsi “transmutés", les mesures de radioactivité induite vont permettre de déterminer la composition de l'échantillon irradié. Cette méthode dite d'analyse par activation neutronique est pratiquée depuis la découverte du neutron. Elle a permis grâce à sa sélectivité et à sa sensibilité d'avoir accès au domaine des traces et des ultra-traces dans des champs d'application très divers comme la métallurgie, l'archéologie, la biologie, la géochimie etc...

  5. Ion-exchange resin separation applied to activation analysis (1963); Separation par resines echangeuses d'ions appliquees a l'analyse par activation (1963)

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    Aubouin, G; Laverlochere, J [Commissariat a l' Energie Atomique, Grenoble (France). Centre d' Etudes Nucleaires

    1963-07-01

    The separation techniques based on ion-exchange resins have been used, in this study, for carrying out activation analyses on about thirty impurities. A separation process has been developed so as to standardise these analyses and to render them execution a matter of routine. The reparation yields obtained are excellent and make it possible to carry out analyses on samples having a large activation cross-section ween working inside a reinforced fume-cupboard. This technique has been applied to the analysis of impurities in tantalum, iron, gallium, germanium, terphenyl, and tungsten. The extension of this process to other impurities and to other matrices is now being studied. (authors) [French] Les techniques de separations sur resines echangeusee d'ions ont ete utilisees, dans cette etude, pour effectuer des analyses par activation sur une trentaine d'impuretes. Un schema de separation a ete mis au point de maniere a normaliser ces analyses et a pouvoir les faire en routine. Les rendements de separation obtenus sont excellents et permettent de proceder a des analyses d'echantillons a grande section efficace d'activation en travaillant dans une sorbonne blindee. Des applications de cette technique ont ete faites pour des analyses d'impuretes dans le tantale, le fer, le gallium, le germanium, le terphenyle, le tungstene. L'extension de ce schema a d'autres impuretes et a d'autres matrices est en cours d'etude. (auteurs)

  6. Ion-exchange resin separation applied to activation analysis (1963); Separation par resines echangeuses d'ions appliquees a l'analyse par activation (1963)

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    Aubouin, G.; Laverlochere, J. [Commissariat a l' Energie Atomique, Grenoble (France). Centre d' Etudes Nucleaires

    1963-07-01

    The separation techniques based on ion-exchange resins have been used, in this study, for carrying out activation analyses on about thirty impurities. A separation process has been developed so as to standardise these analyses and to render them execution a matter of routine. The reparation yields obtained are excellent and make it possible to carry out analyses on samples having a large activation cross-section ween working inside a reinforced fume-cupboard. This technique has been applied to the analysis of impurities in tantalum, iron, gallium, germanium, terphenyl, and tungsten. The extension of this process to other impurities and to other matrices is now being studied. (authors) [French] Les techniques de separations sur resines echangeusee d'ions ont ete utilisees, dans cette etude, pour effectuer des analyses par activation sur une trentaine d'impuretes. Un schema de separation a ete mis au point de maniere a normaliser ces analyses et a pouvoir les faire en routine. Les rendements de separation obtenus sont excellents et permettent de proceder a des analyses d'echantillons a grande section efficace d'activation en travaillant dans une sorbonne blindee. Des applications de cette technique ont ete faites pour des analyses d'impuretes dans le tantale, le fer, le gallium, le germanium, le terphenyle, le tungstene. L'extension de ce schema a d'autres impuretes et a d'autres matrices est en cours d'etude. (auteurs)

  7. Use of particles other than neutrons in activation analysis; Emploi de particules autres que les neutrons en analyse par actuation

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    Engelmann, Ch [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

    1964-07-15

    Nuclear reactions obtained by irradiation in {gamma} Bremsstrahlung, {alpha} particles and protons are particularly suitable for dosing very small traces of light elements. We consider the possibilities presented by activation in {gamma} radiation of 28 MeV maximum energy, mainly for the measurement of C, F, N, O, P and S. Non-destructive methods of analysis for beryllium are described. Under certain conditions they may also be used for other elements such as B, Ca, Li and Na. We give also the results of our first experiments carried out in an attempt to find a method for dosing carbon and oxygen by irradiation in {alpha} particles and protons. For each type of activation the possible types of interference with other nuclear refections are considered. (author) [French] Des reactions nucleaires obtenues par irradiation dans des rayons {gamma} de freinage, des particules {alpha} et des protons, sont particulierement indiquees pour les dosages de traces ultimes de certains elements legers. Nous etudions les possibilites offertes par les activations en rayons {alpha} d'energie maximum 28 MeV, principalement pour les dosages de C, F, N, O, P et S. Des methodes d'analyse non destructives appliquees au beryllium sont decrites. Sous certaines conditions, elles peuvent egalement etre utilisees pour d'autres materiaux comme B, Ca, Li et Na. Nous donnons d'autre part les resultats de nos premieres experiences effectuees pour la mise au point des methodes de dosage du carbone et de l'oxygene par irradiation dans les particules {alpha} et les protons. Pour chaque type d'activation, les possibilites d'interferences avec d'autres reactions nucleaires sont examinees. (auteur)

  8. Determination of the sanguine iodine content by activation analysis (1962); Determination de l'iode sanguin par analyse d'activation (1962)

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    Kellershohn, C; Comar, D; Le Poec, C [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

    1962-07-01

    Two methods of measuring the iodine content of the blood by activation analysis after 30 min irradiation in a flux of 6.10{sup 12} n/cm{sup 2}.s are described. One method includes a chemical separation of iodine before irradiation. The radioactivity of this specimen is determined on the basis of the amplitude of the iodine 128 photoelectric peak measured by {gamma}-spectrometry. The sensitivity of this method is about 10{sup -8} g. The other includes chemical separation after irradiation, and radioactivity is subsequently measured with a Geiger-Muller counter. The sensitivity of this second method is about 10{sup -10} g, sufficient for determination of the plasmatic mineral content. The advantages of activation analysis over the conventional method based on colorimetric measurement of the catalytic action of iodine in the oxidation of arsenious anhydride with ceric sulphate are discussed. Initial results obtained with this new form of analysis are detailed as are also the possibilities of measuring plasmatic proteic iodine by scintillation spectrometry without prior chemical separation. (authors) [French] Deux methodes de dosage de l'iode sanguin par analyse d'activation apres irradiation de 30 minutes a 6.10{sup 12} n/cm{sup 2} s sont decrites. L'une comprend une separation chimique de l'iode avant l'irradiation et la mesure de la radioactivite est effectuee a partir de l'amplitude du pic photo-electrique de l'iode 128 obtenu par spectrometrie-{gamma}. Sa sensibilite est de l'ordre de 10{sup -8} g. L'autre comprend une separation chimique de l'iode apres l'irradiation et la radioactivite de l'iode-128 est mesuree par comptage-{beta} avec un compteur Geiger. Sa sensibilite est de l'ordre de 10{sup -10} g, permettant d'envisager le dosage de l'iode mineral plasmatique. Les avantages de l'analyse d'activation sur la methode classique, basee sur la mesure colorimetrique de l'action catalytique de l'iode dans l'oxydation de l'anhydride arsenieux par le sulfate cerique

  9. Argon activation analysis, application to dating by the potassium-argon method; Analyse par activation de l'argon. Application a la datation par la methode potassium-argon

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    Dumesnil, P. [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

    1967-07-01

    Activation analysis using radiogenic argon-40 has been applied to rock-dating by the K-Ar method. The argon is extracted from the sample, purified, activated to saturation in a flux of 2 X 10{sup 13} neutrons/cm{sup 2} sec{sup -1} and measured by gamma spectroscopy. The sensitivity obtained is such that it is possible to measure amounts of argon corresponding to age of a few thousand years only. However since it has not been possible to measure the amount of pollution of radiogenic argon by atmospheric argon with any accuracy, the measurable age limit is in fact of the order of MY. The method has been applied to basalts from the Mont-Dore region. The results obtained are in fairly good agreement with geological, stratigraphic and paleomagnetic data. (author) [French] L'analyse par activation de l'argon 40 radiogenique a ete appliquee a la datation des roches par la methode K-Ar. L'argon est extrait de l'echantillon, purifie, active a saturation dans un flux de 2.10{sup 13} neutrons.cm{sup -2}.s{sup -1} et mesure en spectrometrie gamma. La sensibilite obtenue est telle qu'il est possible de mesurer des quantites d'argon correspondant a des ages de quelques milliers d'annees seulement. Cependant la correction de pollution de l'argon radiogenique par l'argon atmospherique n'ayant pu etre etablie avec precision, la limite d'age mesurable pratique est de l'ordre de 1 Ma. La methode a ete appliquee aux basaltes de la region du Mont-Dore. Les ages obtenus sont en assez bon accord avec les donnees geologiques, stratigraphiques et paleomagnetiques. (auteur)

  10. Argon activation analysis, application to dating by the potassium-argon method; Analyse par activation de l'argon. Application a la datation par la methode potassium-argon

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    Dumesnil, P [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

    1967-07-01

    Activation analysis using radiogenic argon-40 has been applied to rock-dating by the K-Ar method. The argon is extracted from the sample, purified, activated to saturation in a flux of 2 X 10{sup 13} neutrons/cm{sup 2} sec{sup -1} and measured by gamma spectroscopy. The sensitivity obtained is such that it is possible to measure amounts of argon corresponding to age of a few thousand years only. However since it has not been possible to measure the amount of pollution of radiogenic argon by atmospheric argon with any accuracy, the measurable age limit is in fact of the order of MY. The method has been applied to basalts from the Mont-Dore region. The results obtained are in fairly good agreement with geological, stratigraphic and paleomagnetic data. (author) [French] L'analyse par activation de l'argon 40 radiogenique a ete appliquee a la datation des roches par la methode K-Ar. L'argon est extrait de l'echantillon, purifie, active a saturation dans un flux de 2.10{sup 13} neutrons.cm{sup -2}.s{sup -1} et mesure en spectrometrie gamma. La sensibilite obtenue est telle qu'il est possible de mesurer des quantites d'argon correspondant a des ages de quelques milliers d'annees seulement. Cependant la correction de pollution de l'argon radiogenique par l'argon atmospherique n'ayant pu etre etablie avec precision, la limite d'age mesurable pratique est de l'ordre de 1 Ma. La methode a ete appliquee aux basaltes de la region du Mont-Dore. Les ages obtenus sont en assez bon accord avec les donnees geologiques, stratigraphiques et paleomagnetiques. (auteur)

  11. Some applications of neutron activation analysis in plant biology and agronomy; Quelques applications de l'analyse par radioactivation neutronique en biologie vegetale et en agronomie

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    Fourcy, A [Commissariat a l' Energie Atomique, Grenoble (France). Centre d' Etudes Nucleaires

    1966-06-01

    Plants materials are not so commonly analysed by radioactivation than biological extracts of medical importance. With help of concrete examples, applications of neutrons activation analysis to the determination of some metals (Mn, Cu, Co, Fe, Zn, and K) in plant materials, are proposed. Samples are activated in a swimming-pool reactor at the thermal flux of 5.10{sup 12} n.cm{sup -2}s{sup -1} for a time varying between few minutes and several days according to the element being analysed. The induced radioactivity is measured by spectrometry, with radiochemical separation ( Cu, Co, Fe, Zn and K) or without separation in best cases (Mn,Cu, K). Described dosages are related to: manganese in a graminaceous plant, copper in vine treatments, cobalt, iron and zinc in animal feeding, potassium in a radiological experiment. (author) [French] Les produits vegetaux sont beaucoup moins souvent soumis a l'analyse par activation que les extraits biologiques d'interet medical. En s'appuyant sur des cas concrets, nous proposons des applications de l'analyse par activation neutronique au dosage de differents metaux (Mn, Cu, Co, Fe, Zn, et K) dans la matiere vegetale. Les echantillons sont actives en pile piscine au flux thermique de 5.10{sup 12} n.cm{sup -2} s{sup -1} pendant des temps variant de quelques minutes a plusieurs jours selon les elements a analyser. le comptage de la radioactivite induite est effectue par spectrometrie gamma apres separation radiochimique (Cu, Co, Fe, Zn, et K) ou sans separation dans les cas favorables (Mn, Cu, K). Les dosages decrits concernent: le manganese dans une graminee, le cuivre en viticulture, le cobalt, le fer, et le zinc dans un aliment du betail, le potassium dans une experience de radioecologie. (auteur)

  12. Investigation of aluminium and manganese uptake in Congolese flora by activation analysis; Recherche d'accumulateurs D’ Aluminium Et De Manganese Dans La Flore Congolaise Par La Methode D'Analyse Par Activation

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    Evrard, C.; Roels, J.; Pauwels, L.; Pollak, H. [Centre nucléaire TRICO, Kinshasa (Congo, The Democratic Republic of the); Université Lovanium, Kinshasa (Congo, The Democratic Republic of the)

    1970-01-15

    It is known that certain plants accumulate one or more elements in their tissues. This characteristic can make a valuable contribution to the taxonomy of groups, families and even of species. The ecology characteristic of each species may also be demonstrated by the accumulation of certain elements. Soil acidity can have a strong effect on accumulation. High acidity favours the accumulation of manganese, for example. The authors have endeavoured to perfect a technique of analysis based on neutron activation (nondestructive). Thermal neutron activation is carried out in the TRIGA Mk I reactor of the TRICO Nuclear Centre, which has a flux of about 10{sup 11}n cm{sup -2} s{sup -1}. Activation analysis is quicker than chemical methods and has the additional advantage that it can be used for small quantities of plant material. At least forty species of local plants have been tested. In addition to these specific tests, we have analysed leaves from trees of a given species growing in different soils, in order to get some idea of individual variations from one plant to another. (author) [French] Il est connu que certaines plantes accumulent un ou plusieurs éléments dans leurs tissus. Cette particularité peut apporter une précieuse contribution à la taxonomie des groupes, des familles ou même des espèces. L'écologie propre â chaque espèce peut aussi être mise en évidence par l'accumulation de certains éléments. Le degré d'acidité du sol, par exemple, peut fortement influencer cette accumulation. Dans le cas de manganèse, une forte acidité du sol favorise l'accumulation de cet élément. Les auteurs ont cherché à mettre au point une méthode d'analyse par activation neutronique (non destructive). Les activations aux neutrons thermiques ont été effectuées dans le réacteur TRIGA Mk I du Centre nucléaire TRICO, dans un flux d'environ 10{sup 11} n cm{sup -2} sec{sup -1}. Comparée à la méthode chimique, l'analyse par activation est plus rapide et peut

  13. Application of neutron activation analysis to the study of impurities in molybdenum, tungsten and nuclear graphite; Application de l'analyse par activation neutronique a l'etude des impuretes dans le molybdene, le tungstene et le graphite nucleaire

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    Pinte, G [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

    1967-10-15

    A neutron activation method is described for the analysis of a maximum number of foreign elements in molybdenum, tungsten and graphite. The various elements are isolated using a systematic separation programme; the elements are subsequently analysed qualitatively and quantitatively using {gamma}-spectrometry. By this method are dosed 27 elements in molybdenum and tungsten, and 20 elements in graphite to which can be added those elements which are already the object of routine analysis: V, Mn, Si, P, S, Cl and 14 rare earths. (author) [French] On decrit une methode d'analyse par activation neutronique permettant de doser un maximum d'elements etrangers dans le molybdene, le tungstene et le graphite. En suivant un schema de separation systematique, on isole les differents elements dont les analyses qualitatives et quantitatives sont ensuite effectuees par spectrometrie {gamma}. Par cette methode, on dose 27 elements dans le molybdene et le tungstene, 20 elements dans le graphite, auxquels on peut encore ajouter les elements doses couramment: V, Mn, Si, P, S, Cl et 14 Terres Rares. (auteur)

  14. Contribution to the activation analysis of the rare gases. Contribution to the analysis of carbon monoxide and water vapour in gases (1963); Contribution a l'analyse par activation des gaz rares. Contribution a l'analyse de l'oxyde de carbone et de la vapeur d'eau dans les gaz (1963)

    Energy Technology Data Exchange (ETDEWEB)

    Diebolt, J [Commisariat a l' Energie Atomique, Grenoble (France). Centre d' Etudes Nucleaires

    1963-07-15

    In the present work, we have applied radioactive analysis to the general technique of rare gas separation. This separation is carried out on active charcoal, at constant pressure. The desorption of the gas is caused by an increase in the initial temperature of adsorption. These separations are quantitative and can be confirmed by chemical analysis. The volumes of desorbed gas are measured by radioactive counting. We have also used radioactivation to study the reduction of palladous chloride by carbon monoxide. Since this reduction is quantitative, we can have a knowledge of the mass of palladium reduced by measuring its activity. We have used the property which have organic chlorides of being hydrolysed by water vapour and of liberating hydrochloric acid to study quantitatively the amount of water vapour which a gas contains. The hydrochloric acid formed is measured by activation of the chlorine in the acid. (author) [French] Dans les travaux que nous presentons, nous avons applique l'analyse par radioactivation, a la technique generale de separation des gaz rares. Cette separation est faite sur charbon active, a pression constante. La desorption des gaz est provoquee par une augmentation de la temperature initiale d'adsorption. Ces separations sont quantitatives, et peuvent etre exploitees par l'analyse chimique. Les volumes de gaz dedorbes sont mesures par comptage de la radioactivite. Nous avons egalement utilise la radioactivation pour l'etude de la reduction du chlorure palladeux par l'oxyde de carbone. Cette reduction etant quantitative, nous pouvons connaitre la masse de palladium reduit par la mesure de son activite. Nous avons exploite la propriete que possede un chlorure organique d'etre hydrolise par la vapeur d'eau et de liberer de l'acide chlorhydrique pour etudier quantitativement la vapeur d'eau contenue dans un gaz. L'acide chlorhydrique forme est mesure par l'activation du chlore de l'acide. (auteur)

  15. Measurement and analysis of high energy radiation through activation detectors. Application in dosimetry; Sur la mesure et l'analyse des rayonnements de haute energie par detecteurs a activation. Application a la dosimetrie

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    Sklavenitis, L. [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

    1967-10-15

    This work is concerned with the possibility of measurement and analysis of radiation fluences within objects of small volume submitted to a high energy proton beam. The first part, consecrated to the establishment of a method of analysis, comprises a detailed study of the radiation nature and energy spectra as well as of the various dosimetry methods. In order to select a group of detectors, high energy nuclear reactions were systematically studied and for some of them cross sections were measured or calculated: for example the cross section of the reaction {sup 11}B (p,n) {sup 11}C between 150 and 3000 MeV and of the reaction {sup 34}S (p,2pn) {sup 32}P between 50 and 3000 MeV. The second part is relative to the application of the fore-mentioned analysis to radiation within a tissue equivalent phantom irradiated by 3 GeV protons. This analysis is sufficiently detailed to allow the reconstitution of the absorbed doses, the dose equivalent and, contingent on a better knowledge of the dose due to heavy particles, the quality factors. It allowed also to follow the evolution of the various dosimetric data as a function of the depth inside the phantom and to verify calculations already done by other researchers. The comparison of the measured doses and the corresponding detector activities revealed the possibility that some detectors could give directly the absorbed dose, or even the dose equivalent, by a simple activity measurement. (author) [French] Le travail porte sur la possibilite de mesure et d'analyse, a l'aide de detecteurs a activation, des fluences de rayonnements a l'interieur d'un objet de petit volume soumis a un faisceau de protons de tres haute energie. La premiere partie, consacree a la mise au point de la methode d'analyse des fluences, comporte une etude detaillee de la nature des rayonnements et de leurs spectres energetiques ainsi que des differentes methodes de dosimetrie. Pour arriver au choix d'un groupe de

  16. Measurement and analysis of high energy radiation through activation detectors. Application in dosimetry; Sur la mesure et l'analyse des rayonnements de haute energie par detecteurs a activation. Application a la dosimetrie

    Energy Technology Data Exchange (ETDEWEB)

    Sklavenitis, L [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

    1967-10-15

    This work is concerned with the possibility of measurement and analysis of radiation fluences within objects of small volume submitted to a high energy proton beam. The first part, consecrated to the establishment of a method of analysis, comprises a detailed study of the radiation nature and energy spectra as well as of the various dosimetry methods. In order to select a group of detectors, high energy nuclear reactions were systematically studied and for some of them cross sections were measured or calculated: for example the cross section of the reaction {sup 11}B (p,n) {sup 11}C between 150 and 3000 MeV and of the reaction {sup 34}S (p,2pn) {sup 32}P between 50 and 3000 MeV. The second part is relative to the application of the fore-mentioned analysis to radiation within a tissue equivalent phantom irradiated by 3 GeV protons. This analysis is sufficiently detailed to allow the reconstitution of the absorbed doses, the dose equivalent and, contingent on a better knowledge of the dose due to heavy particles, the quality factors. It allowed also to follow the evolution of the various dosimetric data as a function of the depth inside the phantom and to verify calculations already done by other researchers. The comparison of the measured doses and the corresponding detector activities revealed the possibility that some detectors could give directly the absorbed dose, or even the dose equivalent, by a simple activity measurement. (author) [French] Le travail porte sur la possibilite de mesure et d'analyse, a l'aide de detecteurs a activation, des fluences de rayonnements a l'interieur d'un objet de petit volume soumis a un faisceau de protons de tres haute energie. La premiere partie, consacree a la mise au point de la methode d'analyse des fluences, comporte une etude detaillee de la nature des rayonnements et de leurs spectres energetiques ainsi que des differentes methodes de dosimetrie. Pour arriver au choix d'un groupe de detecteurs, une etude systematique des

  17. Morphology and physiology characterization of activated sludge by image analysis; Caracterisation morphologique et physiologique de la biomasse des boues activees par analyse d'images

    Energy Technology Data Exchange (ETDEWEB)

    Pandolfi, D.

    2006-01-15

    The biological reactors of wastewater treatment by activated sludge are hosting a complex ecosystem, composed of different types of bacteria (filamentous, non filamentous), protozoa and metazoa. A good balance between the different types of bacteria is essential to achieve good settleability properties for the flocs in the clarifier. The flocs are formed by filamentous bacteria and exo-polymers produced by zoogleal bacteria. When filamentous bacteria are in excess, the floes do not settle correctly (bulking and foaming phenomena). Methods based on image analysis have been used to detect and characterize automatically filamentous bacteria as well as the abundance of exo-polymers. Monochrome or colour-images have been obtained by optical microscopy with classical (Gram, Neisser, Soudan Black) and fluorescent staining (BacLight, FISH). The validation of the different procedures was achieved on various full-scale treatment plants in Lorraine. Further experiments were carried out on lab-scale continuous and sequential reactors, in order to observe the effect of substrates, of temperature and starvation on the behavior of filamentous microorganisms. Under continuous or sequential aeration, for the same substrate, different Gram characteristics are observed. The study carried out on extracellular polymers with varying carbon/nitrogen ratios reveals that an excess of carbonaceous substance favors the production of polymers, which are essential for flocculation. The results obtained with the Gram and PHB (presence of lipids) procedures in condition of environmental stress (starvation and temperature) have shown that the populations have experienced structural alterations of their membranes. We have noticed that in periods of aerobic starvation the lipids which were present were used in ambient temperature, whereas in anaerobic conditions the polyphosphates were consumed. In absence or presence oxygen at a temperature of 4 deg C, it seems easier for filamentous bacteria to

  18. Morphology and physiology characterization of activated sludge by image analysis; Caracterisation morphologique et physiologique de la biomasse des boues activees par analyse d'images

    Energy Technology Data Exchange (ETDEWEB)

    Pandolfi, D

    2006-01-15

    The biological reactors of wastewater treatment by activated sludge are hosting a complex ecosystem, composed of different types of bacteria (filamentous, non filamentous), protozoa and metazoa. A good balance between the different types of bacteria is essential to achieve good settleability properties for the flocs in the clarifier. The flocs are formed by filamentous bacteria and exo-polymers produced by zoogleal bacteria. When filamentous bacteria are in excess, the floes do not settle correctly (bulking and foaming phenomena). Methods based on image analysis have been used to detect and characterize automatically filamentous bacteria as well as the abundance of exo-polymers. Monochrome or colour-images have been obtained by optical microscopy with classical (Gram, Neisser, Soudan Black) and fluorescent staining (BacLight, FISH). The validation of the different procedures was achieved on various full-scale treatment plants in Lorraine. Further experiments were carried out on lab-scale continuous and sequential reactors, in order to observe the effect of substrates, of temperature and starvation on the behavior of filamentous microorganisms. Under continuous or sequential aeration, for the same substrate, different Gram characteristics are observed. The study carried out on extracellular polymers with varying carbon/nitrogen ratios reveals that an excess of carbonaceous substance favors the production of polymers, which are essential for flocculation. The results obtained with the Gram and PHB (presence of lipids) procedures in condition of environmental stress (starvation and temperature) have shown that the populations have experienced structural alterations of their membranes. We have noticed that in periods of aerobic starvation the lipids which were present were used in ambient temperature, whereas in anaerobic conditions the polyphosphates were consumed. In absence or presence oxygen at a temperature of 4 deg C, it seems easier for filamentous bacteria to

  19. Protease-activated receptor (PAR)-2 is required for PAR-1 signalling in pulmonary fibrosis

    NARCIS (Netherlands)

    Lin, Cong; von der Thüsen, Jan; Daalhuisen, Joost; ten Brink, Marieke; Crestani, Bruno; van der Poll, Tom; Borensztajn, Keren; Spek, C. Arnold

    2015-01-01

    Idiopathic pulmonary fibrosis is the most devastating diffuse fibrosing lung disease of unknown aetiology. Compelling evidence suggests that both protease-activated receptor (PAR)-1 and PAR-2 participate in the development of pulmonary fibrosis. Previous studies have shown that bleomycin-induced

  20. Neutron activation analysis of ancient silver coins; Analyse d'anciennes pieces d'argent par activation neutronique; Nejtronozyj aktivatsionnyj analiz starinnykh serebryanykh monet; Analisis de antiguas monedas de plata por activacion neutronica

    Energy Technology Data Exchange (ETDEWEB)

    Aitken, Martin J; Emeleus, Vera M; Hall, Edward T [Research Laboratory for Archaeology and the History of Art, University of Oxford (United Kingdom); Kraay, Colin M [Heberden Coin Room, Ashmolean Museum, University of Oxford (United Kingdom)

    1962-01-15

    The amounts of gold and copper present as impurities in 500 Creek silver coins of the fifth century B.C. have been determined with a gamma-ray spectrometer, following neutron activation. The coinage from eight cities and kingdoms was studied; the average gold-content for different groups of coins varies between 0.02% and 0.3%, and the copper content between 0.1% and 10%. Evidence about trading connexions and of deliberate debasements of the coinage has been obtained, and several unsuspected plated coins were detected. The gold content was determined by measuring the intensity of the 0.411 MeV gamma-ray from Au{sup 198} (2.69 d); for the copper content the 0.511 MeV positron annihilation radiation from Cu{sup 64} (12.8 h) was used, and for silver the 0.884 MeV gamma-ray from Ag{sup 110m} (253 d). Decay measurements were used as a check of identity. The technique of using total gamma-activity decay curves by themselves is insufficiently sensitive. For accurate work, the importance of approximate facsimile standards is stressed. (author) [French] Apres activation neutronique, on a pu determiner, a l'aide d'un spectrometre gamma, les quantites d'or et de cuivre contenues comme impuretes dans 500 pieces d'argent grecques du cinquieme siecle avant J.C. On a etudie des pieces frappees par huit cites ou royaumes; la teneur moyenne en or des differentes pieces varie de 0,02 a 0,3%, tandis que la teneur en cuivre se situe entre 0,1 et 10%. Ces pieces ont permis, d'une part, de se rendre compte des relations commerciales existant a l'epoque et, d'autre part, de constater que certaines d'entre elles avaient ete deliberement alterees et que plusieurs autres etaient simplement plaquees. On a determine la teneur en or en mesurant l'intensite des rayons gamma de 0,411 MeV emis par l'or-198 (2,69 jours); pour la teneur en cuivre, on a utilise les rayonnements d'annihilation des positons de 0,511 MeV emis par le cuivre-64 (12,8 heures); pour la teneur en argent, les rayons gamma de

  1. Protease activated receptors (PARS) mediation in gyroxin biological activity

    International Nuclear Information System (INIS)

    Silva, Jose Alberto Alves da

    2009-01-01

    Gyroxin is a serine protease enzyme from the South American rattlesnake (Crotalus durissus terrificus) venom; it is only partially characterized and has multiple activities. Gyroxin induces blood coagulation, blood pressure decrease and a neurotoxic behavior named barrel rotation. The mechanisms involved in this neurotoxic activity are not known. Whereas gyroxin is a member of enzymes with high potential to become a new drug with clinical applications such as thrombin, batroxobin, ancrod, tripsyn and kalicrein, it is important to find out how gyroxin works. The analysis on agarose gel electrophoresis and circular dichroism confirmed the molecules' integrity and purity. The gyroxin intravenous administration in mice proved its neurotoxicity (barrel rotation). In vivo studies employing intravital microscopy proved that gyroxin induces vasodilation with the participation of protease activated receptors (PARs), nitric oxide and Na+K+ATPase. The leukocytes' adherence and rolling counting indicated that gyroxin has no pro inflammatory activity. Gyroxin induced platelet aggregation, which was blocked by inhibitors of PAR1 and PAR4 receptors (SCH 79797 and tcY-NH 2 , respectively). Finally, it was proved that the gyroxin temporarily alter the permeability of the blood brain barrier (BBB). Our study has shown that both the protease-activated receptors and nitric oxide are mediators involved in the biological activities of gyroxin. (author)

  2. PAR1 activation affects the neurotrophic properties of Schwann cells.

    Science.gov (United States)

    Pompili, Elena; Fabrizi, Cinzia; Somma, Francesca; Correani, Virginia; Maras, Bruno; Schininà, Maria Eugenia; Ciraci, Viviana; Artico, Marco; Fornai, Francesco; Fumagalli, Lorenzo

    2017-03-01

    Protease-activated receptor-1 (PAR1) is the prototypic member of a family of four G-protein-coupled receptors that signal in response to extracellular proteases. In the peripheral nervous system, the expression and/or the role of PARs are still poorly investigated. High PAR1 mRNA expression was found in the rat dorsal root ganglia and the signal intensity of PAR1 mRNA increased in response to sciatic nerve transection. In the sciatic nerve, functional PAR1 receptor was reported at the level of non-compacted Schwann cell myelin microvilli of the nodes of Ranvier. Schwann cells are the principal population of glial cells of the peripheral nervous system which myelinate axons playing an important role during axonal regeneration and remyelination. The present study was undertaken in order to determine if the activation of PAR1 affects the neurotrophic properties of Schwann cells. Our results suggest that the stimulation of PAR1 could potentiate the Schwann cell ability to favour nerve regeneration. In fact, the conditioned medium obtained from Schwann cell cultures challenged with a specific PAR1 activating peptide (PAR1 AP) displays increased neuroprotective and neurotrophic properties with respect to the culture medium from untreated Schwann cells. The proteomic analysis of secreted proteins in untreated and PAR1 AP-treated Schwann cells allowed the identification of factors differentially expressed in the two samples. Some of them (such as macrophage migration inhibitory factor, matrix metalloproteinase-2, decorin, syndecan 4, complement C1r subcomponent, angiogenic factor with G patch and FHA domains 1) appear to be transcriptionally regulated after PAR1 AP treatment as shown by RT-PCR. Copyright © 2017 Elsevier Inc. All rights reserved.

  3. Analytical studies by activation. Part A and B: Counting of short half-life radio-nuclides. Part C: Analytical programs for decay curves; Etudes d'analyse par activation. Parties A et B: le comptage des radio-nucleides de periodes courtes. Partie C: programme de depouillement des courbes de decroissance

    Energy Technology Data Exchange (ETDEWEB)

    Junod, E [Commissariat a l' Energie Atomique Grenoble (France). Centre d' Etudes Nucleaires

    1966-03-01

    est consacree au probleme des corrections de pertes au comptage dues au temps mort des analyseurs multicanaux. On a etabli les formules de correction exactes lorsque le radionucleide de periode courte est pur ou seulement accompagne d'un radionucleide de periode longue. Par comparaison on a trace sous forme d'abaques les approximations fournies par le comptage dit 'en temps actif' et par un mode de comptage en temps reel associe a la mesure du temps mort global, ce second procede se revelant plus valable que le premier. Dans le cas d'un melange complexe, on donne le moyen de le ramener au probleme a deux constituants. Partie C: On presente les problemes que pose l'analyse qualitative et quantitative des courbes de decroissance d'un melange de sources radioactives dont au moins une de periode courte. Six procedes fondamentaux sont decrits mathematiquement pour lesquels sont proposes des elements de programmes fortran. Deux programmes supplementaires sont construits pour traiter globalement des problemes de dosages en analyse par activation: l'un base sur l'irradiation simultanee d'un echantillon et d'un ou plusieurs temoins; l'autre sur l'irradiation separee de l'echantillon et du temoin, un dosimetre (par activation ou exterieur) servant a normaliser les conditions de flux d'irradiation.

  4. Use of 14 MeV neutrons in activation analysis. Experiments carried out at the Grenoble nuclear research Centre (1963); Utilisation de neutrons de 14 MeV en analyse par activation. Experience realisee au Centre d'etudes nucleaires de Grenoble (1963)

    Energy Technology Data Exchange (ETDEWEB)

    Auboin, G; Guazzoni, P; Laverlochere, J [Commisariat a l' Energie Atomique, Grenoble (France). Centre d' Etudes Nucleaires

    1963-07-01

    The use of 14 MeV neutron irradiation makes it possible to extend the field of application of activation analysis to elements which cannot be activated in nuclear reactors (oxygen, boron, for example) or else to consider the possibility of 'portable' measurement units for routine analyses. With this aim, tests have been carried out for studying the sensitivity and interferences in the case of the dosing of oxygen in eleven different matrices using 150 and 300 kV SAMES accelerators producing 14 MeV neutrons by the reaction {sup 3}H (d, n) {sup 4}He. The sensitivity of the dosage methods for six writer elements has also been studied. The results obtained show that, for a neutron flux of 10{sup 10} n/4{pi}, the sensitivities vary from 100 {mu}g and 1 mg for oxygen in nine matrices (Be, Ta, Nb, Ti, Zr, Fe, Al, Sb, Si) and that it is possible to dose the six elements F, B, Y, Si, N, Al with sensitivities of between 1 an 1000 micrograms. (authors) [French] L'irradiation au moyen de neutrons de 14 MeV permet d'etendre le domaine d'application de l'analyse par activation a des elements non activables en reacteur nucleaire (oxygene, bore, par exemple) ou encore d'envisager des unites de dosage ''portables'' pour des analyses de routine. Dans ce but, des essais ont ete effectues pour etudier les sensibilites et les interferences dans le cas du dosage de l'oxygene dans onze matrices differentes, au moyen d'accelerateurs SAMES 150 et 300 kV. produisant les neutrons de 14 MeV par la reaction {sup 3}H (d, n) {sup 4}He. Des sensibilites de dosage ont egalement ete etudiees pour six autres elements. Les resultats obtenus montrent que, pour un flux de neutrons de 10{sup 10} n /4{pi}, les sensibilites varient entre 100 {mu}g et 1 mg, pour l'oxygene dans neuf matrices (Be, Ta, Nb, Ti, Zr, Fe, Al, Sb, Si) et qu'il est possible de doser les six elements F, B, Y, Si, N, Al avec des sensibilites se situant entre 1 et 1000 microgrammes. (auteurs)

  5. Novel role for proteinase-activated receptor 2 (PAR2) in membrane trafficking of proteinase-activated receptor 4 (PAR4).

    Science.gov (United States)

    Cunningham, Margaret R; McIntosh, Kathryn A; Pediani, John D; Robben, Joris; Cooke, Alexandra E; Nilsson, Mary; Gould, Gwyn W; Mundell, Stuart; Milligan, Graeme; Plevin, Robin

    2012-05-11

    Proteinase-activated receptors 4 (PAR(4)) is a class A G protein-coupled receptor (GPCR) recognized through the ability of serine proteases such as thrombin and trypsin to mediate receptor activation. Due to the irreversible nature of activation, a fresh supply of receptor is required to be mobilized to the cell surface for responsiveness to agonist to be sustained. Unlike other PAR subtypes, the mechanisms regulating receptor trafficking of PAR(4) remain unknown. Here, we report novel features of the intracellular trafficking of PAR(4) to the plasma membrane. PAR(4) was poorly expressed at the plasma membrane and largely retained in the endoplasmic reticulum (ER) in a complex with the COPI protein subunit β-COP1. Analysis of the PAR(4) protein sequence identified an arginine-based (RXR) ER retention sequence located within intracellular loop-2 (R(183)AR → A(183)AA), mutation of which allowed efficient membrane delivery of PAR(4). Interestingly, co-expression with PAR(2) facilitated plasma membrane delivery of PAR(4), an effect produced through disruption of β-COP1 binding and facilitation of interaction with the chaperone protein 14-3-3ζ. Intermolecular FRET studies confirmed heterodimerization between PAR(2) and PAR(4). PAR(2) also enhanced glycosylation of PAR(4) and activation of PAR(4) signaling. Our results identify a novel regulatory role for PAR(2) in the anterograde traffic of PAR(4). PAR(2) was shown to both facilitate and abrogate protein interactions with PAR(4), impacting upon receptor localization and cell signal transduction. This work is likely to impact markedly upon the understanding of the receptor pharmacology of PAR(4) in normal physiology and disease.

  6. Isotopic analysis of plutonium by optical spectroscopy; Analyse isotopique du plutonium par spectroscopie optique

    Energy Technology Data Exchange (ETDEWEB)

    Artaud, J; Chaput, M; Gerstenkorn, S [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

    1961-07-01

    Isotopic analysis of mixtures of plutonium 239 and 240 has been carried out by means of the photo-electric spectrometer Fabry-Perot, the source being a hollow cathode cooled by liquid nitrogen. This first research has been concerned with finding among the spectral lines given by the hollow cathode, those suitable for use in analysis. Actually, the relative precision is of the order of 2 per cent (for samples containing 3 per cent of {sup 240}Pu). The study of the reproducibility of the measurements should make it possible to increase the precision; the relative precision which can be expected from this method should be 1 per cent for mixtures containing 1 per cent of {sup 240}Pu. (author) [French] L'analyse isotopique des melanges de plutonium 239 et 240 a ete effectuee a l'aide du spectrometre photoelectrique Fabry-Perot, la source etant une cathode creuse refroidie a l'azote liquide. Ce premier travail a eu pour but de discerner parmi les raies spectrales emises par la cathode creuse, celles susceptibles de servir de raies d'analyse. Actuellement, la precision relative est de l'ordre de 2 pour cent (pour des echantillons contenant 3 pour cent de {sup 240}Pu). L'etude de la reproductibilite des mesures devrait permettre d'ameliorer la precision; la precision relative que l'on pourrait atteindre par cette methode serait de 1 pour cent pour des melanges contenant 1 pour cent de {sup 240}Pu. (auteur)

  7. Isotope analysis by emission spectroscopy; Analyse isotopique par spectroscopie d'emission

    Energy Technology Data Exchange (ETDEWEB)

    Artaud, J; Gerstenkorn, S [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires; Blaise, J [Centre National de la Recherche Scientifique (CNRS), Lab. Aime Cotton, 92 - Meudon-Bellevue (France)

    1959-07-01

    Quantitative analysis of isotope mixtures by emission spectroscopy is resulting from the phenomenon called 'isotope shift', say from the fact that spectral lines produced by a mixture of isotopes of a same element are complex. Every spectral line is, indeed, resulting from several lines respectively corresponding to each isotope. Then isotopic components are near one to others, and their separation is effected by means of Fabry-Perot calibration standard: the apparatus allowing to measure abundances is the Fabry-Perot photo-electric spectrometer, designed in 1948 by MM. JACQUINOT and DUFOUR. This method has been used to make abundance determination in the case of helium, lithium, lead and uranium. In the case of lithium, the utilised analysis line depends on the composition of examined isotopic mixture. For mixtures containing 7 to 93 pour cent of one of isotopes of lithium, this line is the lithium blue line: {lambda} = 4603 angstrom. In other cases the red line {lambda} = 6707 angstrom is preferable, though it allows to do easily nothing but relative determinations. Helium shows no particular difficulty and the analysis line selected was {lambda} = 6678 angstrom. For lead the line {lambda} = 5201 angstrom gives the possibility to determine the isotope abundance for the four isotopes of lead notwithstanding the presence of hyperfine structure of {sup 207}Pb. For uranium, line {lambda} 5027 angstrom is used, and this method allows to determine the composition of isotope mixtures, the content of which in {sup 235}U may shorten to 0,1 per cent. Relative precision is about 2 per cent for contents in {sup 235}U over 1 per cent. For lower contents, this line {lambda} = 5027 angstrom will allow relative measures when using previously dosed mixtures. (author) [French] L'analyse quantitative des melanges isotopiques par spectroscopie d'emission doit son existence au phenomene appele 'deplacement isotopique', c'est-a-dire au fait que les raies spectrales emises par un

  8. Comprendre le développement professionnel par l’analyse des discours

    Directory of Open Access Journals (Sweden)

    Sabine Vanhulle

    2011-07-01

    Full Text Available Dans un dispositif consacré au développement professionnel, nos étudiants en enseignement secondaire tiennent un portfolio. Celui-ci se clôture par un récit de trajectoire. Notre recherche réside dans l’analyse de ces récits à partir d’une grille d’indicateurs linguistiques. Ces indicateurs nous informent sur les processus réflexifs et discursifs à l’œuvre dans l’élaboration de savoirs professionnels. Ce premier niveau de description conduit à une méthode de recherche herméneutique. Il s’agit alors de mettre en lumière les préoccupations sous-jacentes à ces processus : systèmes de valeurs, de motivations et d’intentions ; tensions entre les savoirs scientifiques et la confrontation aux réalités vécues dans l’expérience ; constructions identitaires conflictuelles. Nous questionnons les conditions sociales externes et propres au contexte de formation qui influencent la production de ces récits. Nous en dégageons les implications méthodologiques pour l’analyse de discours à visée descriptive et compréhensive. Sur cette base, nous discutons quelques résultats de la recherche.Mots-clés : Portfolio. Savoirs professionnels écrits. Indicateurs de développement. Formation des valeurs. Herméneutique.

  9. Protease-activated receptor (PAR2, but not PAR1, is involved in collateral formation and anti-inflammatory monocyte polarization in a mouse hind limb ischemia model.

    Directory of Open Access Journals (Sweden)

    Lisa G van den Hengel

    Full Text Available AIMS: In collateral development (i.e. arteriogenesis, mononuclear cells are important and exist as a heterogeneous population consisting of pro-inflammatory and anti-inflammatory/repair-associated cells. Protease-activated receptor (PAR1 and PAR2 are G-protein-coupled receptors that are both expressed by mononuclear cells and are involved in pro-inflammatory reactions, while PAR2 also plays a role in repair-associated responses. Here, we investigated the physiological role of PAR1 and PAR2 in arteriogenesis in a murine hind limb ischemia model. METHODS AND RESULTS: PAR1-deficient (PAR1-/-, PAR2-deficient (PAR2-/- and wild-type (WT mice underwent femoral artery ligation. Laser Doppler measurements revealed reduced post-ischemic blood flow recovery in PAR2-/- hind limbs when compared to WT, while PAR1-/- mice were not affected. Upon ischemia, reduced numbers of smooth muscle actin (SMA-positive collaterals and CD31-positive capillaries were found in PAR2-/- mice when compared to WT mice, whereas these parameters in PAR1-/- mice did not differ from WT mice. The pool of circulating repair-associated (Ly6C-low monocytes and the number of repair-associated (CD206-positive macrophages surrounding collaterals in the hind limbs were increased in WT and PAR1-/- mice, but unaffected in PAR2-/- mice. The number of repair-associated macrophages in PAR2-/- hind limbs correlated with CD11b- and CD115-expression on the circulating monocytes in these animals, suggesting that monocyte extravasation and M-CSF-dependent differentiation into repair-associated cells are hampered. CONCLUSION: PAR2, but not PAR1, is involved in arteriogenesis and promotes the repair-associated response in ischemic tissues. Therefore, PAR2 potentially forms a new pro-arteriogenic target in coronary artery disease (CAD patients.

  10. Protease-Activated Receptor 4 (PAR4): A Promising Target for Antiplatelet Therapy.

    Science.gov (United States)

    Rwibasira Rudinga, Gamariel; Khan, Ghulam Jilany; Kong, Yi

    2018-02-14

    Cardiovascular diseases (CVDs) are currently among the leading causes of death worldwide. Platelet aggregation is a key cellular component of arterial thrombi and major cause of CVDs. Protease-activated receptors (PARs), including PAR1, PAR2, PAR3 and PAR4, fall within a subfamily of seven-transmembrane G-protein-coupled receptors (GPCR). Human platelets express PAR1 and PAR4, which contribute to the signaling transduction processes. In association with CVDs, PAR4 not only contributes to platelet activation but also is a modulator of cellular responses that serve as hallmarks of inflammation. Although several antiplatelet drugs are available on the market, they have many side effects that limit their use. Emerging evidence shows that PAR4 targeting is a safer strategy for preventing thrombosis and consequently may improve the overall cardiac safety profile. Our present review summarizes the PAR4 structural characteristics, activation mechanism, role in the pathophysiology of diseases and understanding the association of PAR4 targeting for improved cardiac protection. Conclusively, this review highlights the importance of PAR4 antagonists and its potential utility in different CVDs.

  11. Protease-Activated Receptor 4 (PAR4: A Promising Target for Antiplatelet Therapy

    Directory of Open Access Journals (Sweden)

    Gamariel Rwibasira Rudinga

    2018-02-01

    Full Text Available Cardiovascular diseases (CVDs are currently among the leading causes of death worldwide. Platelet aggregation is a key cellular component of arterial thrombi and major cause of CVDs. Protease-activated receptors (PARs, including PAR1, PAR2, PAR3 and PAR4, fall within a subfamily of seven-transmembrane G-protein-coupled receptors (GPCR. Human platelets express PAR1 and PAR4, which contribute to the signaling transduction processes. In association with CVDs, PAR4 not only contributes to platelet activation but also is a modulator of cellular responses that serve as hallmarks of inflammation. Although several antiplatelet drugs are available on the market, they have many side effects that limit their use. Emerging evidence shows that PAR4 targeting is a safer strategy for preventing thrombosis and consequently may improve the overall cardiac safety profile. Our present review summarizes the PAR4 structural characteristics, activation mechanism, role in the pathophysiology of diseases and understanding the association of PAR4 targeting for improved cardiac protection. Conclusively, this review highlights the importance of PAR4 antagonists and its potential utility in different CVDs.

  12. Analysis by {alpha} spectrometry (chamber with a grid). Proserpine (1960); Analyses par spectrometrie {alpha} (chambre a grille). Proserpine (1960)

    Energy Technology Data Exchange (ETDEWEB)

    Clouet d' Orval, C [Commissariat a l' Energie Atomique, Saclay (France).Centre d' Etudes Nucleaires

    1960-07-01

    The aim is to compare various substances in an electrolytic deposit by means of their {alpha} radiation. The energies are separated by counting in a chamber with a grid, specially designed for the analysis of plutonium. A resolution of 1.7 per cent is normally obtained for plutonium bands. {sup 239}Pu-{sup 233}Pu, {sup 239}Pu-{sup 238}Pu mixtures have been analysed by this method. (author) [French] Il s'agit de comparer, au moyen de leur rayonnement {alpha}, divers corps contenus dans un depot electrolytique. On separe les energies par comptage dans une chambre a grille, specialement concue pour analyser du plutonium. On obtient couramment une resolution de 1,7 pour cent, pour des raies du plutonium. On a pu faire, par cette methode, l'analyse de melanges {sup 239}Pu-{sup 233}Pu et {sup 239}Pu-{sup 238}Pu. (auteur)

  13. Le dysfonctionnement socio-spatial des grands ensembles en Algérie: technique de l’analyse wayfinding par méthode “movement traces” et l’analyse morphologique (syntaxe spatiale par logiciel “depthmap”

    Directory of Open Access Journals (Sweden)

    Amara Hima

    2018-03-01

    Full Text Available Résumé La technique de l’analyse syntaxique de la visibilité (Visibility Graph Analysis – VGA et de l’accessibilité (All Line Analysis – ALA par logiciel “DepthMap©(UCL, Londres” et l’analyse du dysfonctionnement wayfinding par méthode “movement traces”, sont utilisées dans ce papier afin de développer un modèle d’analyse et d’investigation de l’impact des changements spatiaux sur le dysfonctionnement socio-spatial du wayfinding, ainsi sur la reproduction urbaine, notamment les transformations des façades et l’appropriation des espaces extérieurs dans les grands ensembles en Algérie. Nous donnons ici le cas d’étude de la cité 1000 logt-Biskra et la cité 500 logt-M’sila. Afin de vérifier cette hypothèse, un modèle d’analyse hybride a été développé par croisement des résultats d’analyses des deux techniques. Par conséquent, le schéma de l’interférence montre que la majorité des piétons préfèrent parcourir les axes courts et droits — caractérisés par une forte propriété syntaxique de visibilité et d’accessibilité (l’intégration, la connectivité et l’intelligibilité — en directions des équipements adjacents et aux milieux des deux cités. Ces itinéraires ont un impact sur les transformations des façades et l’appropriation des espaces extérieurs. Le modèle développé promet de futures recherches sur le plan de la quantification, la modélisation et la simulation du processus de la reproduction urbaine, notamment par les automates cellulaires.

  14. Etk/Bmx regulates proteinase-activated-receptor1 (PAR1 in breast cancer invasion: signaling partners, hierarchy and physiological significance.

    Directory of Open Access Journals (Sweden)

    Irit Cohen

    Full Text Available BACKGROUND: While protease-activated-receptor 1 (PAR(1 plays a central role in tumor progression, little is known about the cell signaling involved. METHODOLOGY/PRINCIPAL FINDINGS: We show here the impact of PAR(1 cellular activities using both an orthotopic mouse mammary xenograft and a colorectal-liver metastasis model in vivo, with biochemical analyses in vitro. Large and highly vascularized tumors were generated by cells over-expressing wt hPar1, Y397Z hPar1, with persistent signaling, or Y381A hPar1 mutant constructs. In contrast, cells over-expressing the truncated form of hPar1, which lacks the cytoplasmic tail, developed small or no tumors, similar to cells expressing empty vector or control untreated cells. Antibody array membranes revealed essential hPar1 partners including Etk/Bmx and Shc. PAR(1 activation induces Etk/Bmx and Shc binding to the receptor C-tail to form a complex. Y/A mutations in the PAR(1 C-tail did not prevent Shc-PAR(1 association, but enhanced the number of liver metastases compared with the already increased metastases obtained with wt hPar1. We found that Etk/Bmx first binds via the PH domain to a region of seven residues, located between C378-S384 in PAR(1 C-tail, enabling subsequent Shc association. Importantly, expression of the hPar1-7A mutant form (substituted A, residues 378-384, which is incapable of binding Etk/Bmx, resulted in inhibition of invasion through Matrigel-coated membranes. Similarly, knocking down Etk/Bmx inhibited PAR(1-induced MDA-MB-435 cell migration. In addition, intact spheroid morphogenesis of MCF10A cells is markedly disrupted by the ectopic expression of wt hPar1. In contrast, the forced expression of the hPar1-7A mutant results in normal ball-shaped spheroids. Thus, by preventing binding of Etk/Bmx to PAR(1 -C-tail, hPar1 oncogenic properties are abrogated. CONCLUSIONS/SIGNIFICANCE: This is the first demonstration that a cytoplasmic portion of the PAR(1 C-tail functions as a scaffold

  15. Profiling gene expression induced by protease-activated receptor 2 (PAR2 activation in human kidney cells.

    Directory of Open Access Journals (Sweden)

    Jacky Y Suen

    Full Text Available Protease-Activated Receptor-2 (PAR2 has been implicated through genetic knockout mice with cytokine regulation and arthritis development. Many studies have associated PAR2 with inflammatory conditions (arthritis, airways inflammation, IBD and key events in tumor progression (angiogenesis, metastasis, but they have relied heavily on the use of single agonists to identify physiological roles for PAR2. However such probes are now known not to be highly selective for PAR2, and thus precisely what PAR2 does and what mechanisms of downstream regulation are truly affected remain obscure. Effects of PAR2 activation on gene expression in Human Embryonic Kidney cells (HEK293, a commonly studied cell line in PAR2 research, were investigated here by comparing 19,000 human genes for intersecting up- or down-regulation by both trypsin (an endogenous protease that activates PAR2 and a PAR2 activating hexapeptide (2f-LIGRLO-NH(2. Among 2,500 human genes regulated similarly by both agonists, there were clear associations between PAR2 activation and cellular metabolism (1,000 genes, the cell cycle, the MAPK pathway, HDAC and sirtuin enzymes, inflammatory cytokines, and anti-complement function. PAR-2 activation up-regulated four genes more than 5 fold (DUSP6, WWOX, AREG, SERPINB2 and down-regulated another six genes more than 3 fold (TXNIP, RARG, ITGB4, CTSD, MSC and TM4SF15. Both PAR2 and PAR1 activation resulted in up-regulated expression of several genes (CD44, FOSL1, TNFRSF12A, RAB3A, COPEB, CORO1C, THBS1, SDC4 known to be important in cancer. This is the first widespread profiling of specific activation of PAR2 and provides a valuable platform for better understanding key mechanistic roles of PAR2 in human physiology. Results clearly support the development of both antagonists and agonists of human PAR2 as potential disease modifying therapeutic agents.

  16. Proteinase-Activated Receptor-1 and Immunomodulatory Effects of a PAR1-Activating Peptide in a Mouse Model of Prostatitis

    Science.gov (United States)

    Stanton, M. Mark; Nelson, Lisa K.; Benediktsson, Hallgrimur; Hollenberg, Morley D.; Buret, Andre G.; Ceri, Howard

    2013-01-01

    Background. Nonbacterial prostatitis has no established etiology. We hypothesized that proteinase-activated receptor-1 (PAR1) can play a role in prostatitis. We therefore investigated the effects of PAR1 stimulation in the context of a new model of murine nonbacterial prostatitis. Methods. Using a hapten (ethanol-dinitrobenzene sulfonic acid- (DNBS-)) induced prostatitis model with both wild-type and PAR1-null mice, we examined (1) the location of PAR1 in the mouse prostate and (2) the impact of a PAR1-activating peptide (TFLLR-NH2: PAR1-TF) on ethanol-DNBS-induced inflammation. Results. Ethanol-DNBS-induced inflammation was maximal at 2 days. In the tissue, PAR1 was expressed predominantly along the apical acini of prostatic epithelium. Although PAR1-TF on its own did not cause inflammation, its coadministration with ethanol-DNBS reduced all indices of acute prostatitis. Further, PAR1-TF administration doubled the prostatic production of interleukin-10 (IL-10) compared with ethanol-DNBS treatment alone. This enhanced IL-10 was not observed in PAR1-null mice and was not caused by the reverse-sequence receptor-inactive peptide, RLLFT-NH2. Surprisingly, PAR1-TF, also diminished ethanol-DNBS-induced inflammation in PAR1-null mice. Conclusions. PAR1 is expressed in the mouse prostate and its activation by PAR1-TF elicits immunomodulatory effects during ethanol-DNBS-induced prostatitis. However, PAR1-TF also diminishes ethanol-DNBS-induced inflammation via a non-PAR1 mechanism by activating an as-yet unknown receptor. PMID:24459330

  17. Plasma levels of intact and cleaved urokinase plasminogen activator receptor (uPAR) in men with clinically localised prostate cancer

    DEFF Research Database (Denmark)

    Kristensen, Gitte; Berg, Kasper Drimer; Lippert, Solvej

    2017-01-01

    Aims: Lymph node metastasis (N1) is an adverse prognostic factor for men with clinically localised prostate cancer (PCa), but the prediction of N1 disease remains difficult. Urokinase plasminogen activator receptor (uPAR) plays an important role in angiogenesis and tumorigenesis. We analysed...... analysis and quantified using the areas under the ROC curve (AUC).Results: All soluble uPAR levels were significantly (p=0.03) higher in patients with N1 disease compared with patients with N0/x disease. ROC curves including clinical tumour stage, biopsy Gleason score, prostate-specific antigen and percent...

  18. PET imaging of urokinase-type plasminogen activator receptor (uPAR) in prostate cancer

    DEFF Research Database (Denmark)

    Skovgaard, Dorthe; Persson, Morten; Kjaer, Andreas

    2016-01-01

    Overexpression of urokinase-type plasminogen activator receptors (uPAR) represents an important biomarker for aggressiveness in most common malignant diseases, including prostate cancer (PC). Accordingly, uPAR expression either assessed directly in malignant PC tissue or assessed directly in plasma...... and prognostic imaging method. In this review, we will focus on the recent development of uPAR PET and the relevance within prostate cancer imaging. Novel antibody and small-molecule radiotracers-targeting uPAR, including a series of uPAR-targeting PET ligands, based on the high affinity peptide ligand AE105......, have been synthesized and tested in vitro and in vivo in preclinical murine xenograft models and, recently, in a first-ever clinical uPAR PET study in cancer patients, including patients with PC. In this phase I study, a high and specific uptake of the tracer 64Cu-DOTA-AE105 was found in both primary...

  19. Analyse des solides déformables par la méthode des éléments finis

    CERN Document Server

    Bonnet, Marc

    2006-01-01

    Cet ouvrage propose une présentation structurée de la formulation et la mise en œuvre de la simulation numérique par éléments finis en mécanique des solides déformables. Il présente et développe les concepts et techniques permettant la transposition, en termes de codes de calcul de structures mécaniques industrielles, des notions fondamentales de mécanique des milieux continus solides, et ce dans le cadre d'analyses en régimes (a) statique linéaire, (b) quasistatique non-linéaire et (c) dynamique linéaire. L'exposé théorique est complété et illustré au moyen de programmes d'initiation écrits en Matlab (librement accessibles par Internet) mettant en œuvre les notions développées dans cet ouvrage et conçus comme support pratique à un enseignement. Le texte combine ainsi l'exposition des principes et des méthodes avec la présentation détaillée de ces programmes et d'exemples les mettant en œuvre. L'ouvrage est complété d'une annexe écrite par Andrei Constantinescu (directeur de...

  20. Solar PAR and UVR modify the community composition and photosynthetic activity of sea ice algae.

    Science.gov (United States)

    Enberg, Sara; Piiparinen, Jonna; Majaneva, Markus; Vähätalo, Anssi V; Autio, Riitta; Rintala, Janne-Markus

    2015-10-01

    The effects of increased photosynthetically active radiation (PAR) and ultraviolet radiation (UVR) on species diversity, biomass and photosynthetic activity were studied in fast ice algal communities. The experimental set-up consisted of nine 1.44 m(2) squares with three treatments: untreated with natural snow cover (UNT), snow-free (PAR + UVR) and snow-free ice covered with a UV screen (PAR). The total algal biomass, dominated by diatoms and dinoflagellates, increased in all treatments during the experiment. However, the smaller biomass growth in the top 10-cm layer of the PAR + UVR treatment compared with the PAR treatment indicated the negative effect of UVR. Scrippsiella complex (mainly Scrippsiella hangoei, Biecheleria baltica and Gymnodinium corollarium) showed UV sensitivity in the top 5-cm layer, whereas Heterocapsa arctica ssp. frigida and green algae showed sensitivity to both PAR and UVR. The photosynthetic activity was highest in the top 5-cm layer of the PAR treatment, where the biomass of the pennate diatom Nitzschia frigida increased, indicating the UV sensitivity of this species. This study shows that UVR is one of the controlling factors of algal communities in Baltic Sea ice, and that increased availability of PAR together with UVR exclusion can cause changes in algal biomass, photosynthetic activity and community composition. © FEMS 2015. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  1. Tumour Microenvironments Induce Expression of Urokinase Plasminogen Activator Receptor (uPAR) and Concomitant Activation of Gelatinolytic Enzymes

    Science.gov (United States)

    Magnussen, Synnøve; Hadler-Olsen, Elin; Latysheva, Nadezhda; Pirila, Emma; Steigen, Sonja E.; Hanes, Robert; Salo, Tuula; Winberg, Jan-Olof; Uhlin-Hansen, Lars; Svineng, Gunbjørg

    2014-01-01

    Background The urokinase plasminogen activator receptor (uPAR) is associated with poor prognosis in oral squamous cell carcinoma (OSCC), and increased expression of uPAR is often found at the invasive tumour front. The aim of the current study was to elucidate the role of uPAR in invasion and metastasis of OSCC, and the effects of various tumour microenvironments in these processes. Furthermore, we wanted to study whether the cells’ expression level of uPAR affected the activity of gelatinolytic enzymes. Methods The Plaur gene was both overexpressed and knocked-down in the murine OSCC cell line AT84. Tongue and skin tumours were established in syngeneic mice, and cells were also studied in an ex vivo leiomyoma invasion model. Soluble factors derived from leiomyoma tissue, as well as purified extracellular matrix (ECM) proteins, were assessed for their ability to affect uPAR expression, glycosylation and cleavage. Activity of gelatinolytic enzymes in the tissues were assessed by in situ zymography. Results We found that increased levels of uPAR did not induce tumour invasion or metastasis. However, cells expressing low endogenous levels of uPAR in vitro up-regulated uPAR expression both in tongue, skin and leiomyoma tissue. Various ECM proteins had no effect on uPAR expression, while soluble factors originating from the leiomyoma tissue increased both the expression and glycosylation of uPAR, and possibly also affected the proteolytic processing of uPAR. Tumours with high levels of uPAR, as well as cells invading leiomyoma tissue with up-regulated uPAR expression, all displayed enhanced activity of gelatinolytic enzymes. Conclusions Although high levels of uPAR are not sufficient to induce invasion and metastasis, the activity of gelatinolytic enzymes was increased. Furthermore, several tumour microenvironments have the capacity to induce up-regulation of uPAR expression, and soluble factors in the tumour microenvironment may have an important role in the

  2. Tumour microenvironments induce expression of urokinase plasminogen activator receptor (uPAR and concomitant activation of gelatinolytic enzymes.

    Directory of Open Access Journals (Sweden)

    Synnøve Magnussen

    Full Text Available The urokinase plasminogen activator receptor (uPAR is associated with poor prognosis in oral squamous cell carcinoma (OSCC, and increased expression of uPAR is often found at the invasive tumour front. The aim of the current study was to elucidate the role of uPAR in invasion and metastasis of OSCC, and the effects of various tumour microenvironments in these processes. Furthermore, we wanted to study whether the cells' expression level of uPAR affected the activity of gelatinolytic enzymes.The Plaur gene was both overexpressed and knocked-down in the murine OSCC cell line AT84. Tongue and skin tumours were established in syngeneic mice, and cells were also studied in an ex vivo leiomyoma invasion model. Soluble factors derived from leiomyoma tissue, as well as purified extracellular matrix (ECM proteins, were assessed for their ability to affect uPAR expression, glycosylation and cleavage. Activity of gelatinolytic enzymes in the tissues were assessed by in situ zymography.We found that increased levels of uPAR did not induce tumour invasion or metastasis. However, cells expressing low endogenous levels of uPAR in vitro up-regulated uPAR expression both in tongue, skin and leiomyoma tissue. Various ECM proteins had no effect on uPAR expression, while soluble factors originating from the leiomyoma tissue increased both the expression and glycosylation of uPAR, and possibly also affected the proteolytic processing of uPAR. Tumours with high levels of uPAR, as well as cells invading leiomyoma tissue with up-regulated uPAR expression, all displayed enhanced activity of gelatinolytic enzymes.Although high levels of uPAR are not sufficient to induce invasion and metastasis, the activity of gelatinolytic enzymes was increased. Furthermore, several tumour microenvironments have the capacity to induce up-regulation of uPAR expression, and soluble factors in the tumour microenvironment may have an important role in the regulation of posttranslational

  3. Isotopic analysis using optical spectroscopy (1963); Analyse isotopique par spectroscopie optique (1963)

    Energy Technology Data Exchange (ETDEWEB)

    Gerstenkorn, S [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

    1963-07-01

    The isotopic displacement in the atomic lines of certain elements (H, He, Li, Ne, Sr, Hg, Pb, U, Pu) is used for dosing these elements isotopically. The use of the Fabry-Perot photo-electric interference spectrometer is shown to be particularly adapted for this sort of problem: in each case we give on the one hand the essential results obtained with this apparatus, and on the other hand the results previously obtained with a conventional apparatus (grating, photographic plate). These results together give an idea of the possibilities of optical spectroscopy: in the best case, the precision which may be expected is of the order of 1 to 2 per cent for isotopes whose concentration is about 1 per cent. (author) [French] Le deplacement isotopique dans les raies atomiques de certains elements (H, He, Li, Ne, Sr, Hg, Pb, U, Pu) est utilise pour effectuer le dosage isotopique de ces elements. L'utilisation du spectrometre interferentiel Fabry-Perot photoelectrique se revele particulierement adapte a ce genre de probleme: dans chaque cas, nous donnons d'une part l'essentiel des resultats obtenus avec cet appareil, et d'autre part, les resultats obtenus anterieurement a l'aide de l'appareillage classique (reseau, plaque photographique). L'ensemble de ces informations donne une idee des possibilites offertes par la spectroscopie optique: dans le meilleur des cas, la precision que l'on peut esperer est de l'ordre de 1 a 2 pour cent pour des isotopes dont la concentration est de l'ordre de 1 pour cent. (auteur)

  4. The determination of sulphur in materials of high neutron absorption cross-section by fast-neutron activation analysis; Determination du soufre dans les matieres de forte section efficace d'absorption neutronique, au moyen d'une analyse par activation avec des neutrons rapides; Opredelenie sery v materialakh s bol'shim secheniem pogloshcheniya nejtronov metodom aktivatsionnogo analiza bystrykh nejtronov; Determinacion del azufre en sustancias de elevada seccion eficaz de absorcion neutronica mediante analisis por activacion con neutrones rapidos

    Energy Technology Data Exchange (ETDEWEB)

    Gibbons, D; Simpson, H [Wantage Research Laboratory (A.E.R.E.), Wantage, Berks. (United Kingdom)

    1962-01-15

    The production of radiophosphorus by the fast neutron (n, p) reaction on sulphur is used for the determination of sulphur by activation analysis. Using this technique it is possible to determine sulphur in materials of high thermal-neutron absorption cross-section because the absorption cross-sections for fast neutrons are, in general, much lower than those for slow neutrons, so that self-shielding errors are considerably reduced. Interference due to the slow neutron (n, {gamma}) reaction on neutral phosphorus is eliminated by a double irradiation technique involving irradiation in regions of differing slow/fast flux-ratios. The method has been applied to the determination of sulphur in chromium and arsenic. (author) [French] La production de radiophosphore par reaction (n, p) de neutrons rapides sur le soufre est utilisee pour la determination du soufre au moyen d'une analyse par activation. Cette methode permet la determination du soufre dans les matieres de forte section efficace d'absorption des neutrons thermiques; en effet, les sections efficaces d'absorption des neutrons rapides sont, d'une maniere generale, beaucoup plus faibles que pour les neutrons lents, de sorte que les erreurs d'autoprotection se trouvent considerablement reduites. L'interference due a la reaction (n, {gamma}) des neutrons lents sur le phosphore naturel est eliminee par une double irradiation, qui implique une irradiation dans les regions ou le rapport du flux de neutrons lents au flux de neutrons rapides a des valeurs differentes. La methode a ete appliquee a la determination du soufre dans le chrome et l'arsenic. (author) [Spanish] La formacion de fosforo radiactivo por la accion de los neutrones rapidos sobre el azufre, reaccion (n, p), puede servir para determinar este elemento mediante analisis por activacion. El empleo de esta tecnica permite efectuar determinaciones de azufre en materiales de elevada seccion eficaz de absorcion de neutrones termicos porque, en general, las

  5. Analysis of electronic circuits using digital computers; L'analyse des circuits electroniques par les calculateurs numeriques

    Energy Technology Data Exchange (ETDEWEB)

    Tapu, C [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

    1968-07-01

    Various programmes have been proposed for studying electronic circuits with the help of computers. It is shown here how it possible to use the programme ECAP, developed by I.B.M., for studying the behaviour of an operational amplifier from different point of view: direct current, alternating current and transient state analysis, optimisation of the gain in open loop, study of the reliability. (author) [French] Differents programmes ont ete proposes pour l'etude des circuits electroniques a l'aide des calculateurs. On montre comment on peut utiliser le programme ECAP, mis au point par I. B. M., pour etudier le comportement d'un amplificateur operationnel, a differents points de vue: analyse en courant continu, courant alternatif et regime transitoire, optimalisation du gain en boucle ouverte, etude de la fiabilite. (auteur)

  6. Factor Xa stimulates fibroblast procollagen production, proliferation, and calcium signaling via PAR1 activation

    International Nuclear Information System (INIS)

    Blanc-Brude, Olivier P.; Archer, Fabienne; Leoni, Patricia; Derian, Claudia; Bolsover, Steven; Laurent, Geoffrey J.; Chambers, Rachel C.

    2005-01-01

    Fibroblast proliferation and procollagen production are central features of tissue repair and fibrosis. In addition to its role in blood clotting, the coagulation cascade proteinase thrombin can contribute to tissue repair by stimulating fibroblasts via proteolytic activation of proteinase-activated receptor-1 (PAR 1 ). During hemostasis, the coagulation cascade proteinase factor X is converted into factor Xa. We have previously shown that factor Xa upregulates fibroblast proliferation via production of autocrine PDGF. In this study, we further examined the effects of factor Xa on fibroblast function and aimed to identify its signaling receptor. We showed that factor Xa stimulates procollagen promoter activity and protein production by human and mouse fibroblasts. This effect was independent of PDGF and thrombin production, but dependent on factor Xa proteolytic activity. We also showed that PAR 1 -deficient mouse fibroblasts did not upregulate procollagen production, mobilize cytosolic calcium, or proliferate in response to factor Xa. Desensitization techniques and PAR 1 -specific agonists and inhibitors were used to demonstrate that PAR 1 mediates factor Xa signaling in human fibroblasts. This is the first report that factor Xa stimulates extracellular matrix production. In contrast with endothelial cells and vascular smooth muscle cells, fibroblasts appear to be the only cell type in which the effects of factor Xa are mediated mainly via PAR 1 and not PAR 2 . These findings are critical for our understanding of tissue repair and fibrotic mechanisms, and for the design of novel approaches to inhibit the profibrotic effects of the coagulation cascade without compromising blood hemostasis

  7. Improved Satellite-based Photosysnthetically Active Radiation (PAR) for Air Quality Studies

    Science.gov (United States)

    Pour Biazar, A.; McNider, R. T.; Cohan, D. S.; White, A.; Zhang, R.; Dornblaser, B.; Doty, K.; Wu, Y.; Estes, M. J.

    2015-12-01

    One of the challenges in understanding the air quality over forested regions has been the uncertainties in estimating the biogenic hydrocarbon emissions. Biogenic volatile organic compounds, BVOCs, play a critical role in atmospheric chemistry, particularly in ozone and particulate matter (PM) formation. In southeastern United States, BVOCs (mostly as isoprene) are the dominant summertime source of reactive hydrocarbon. Despite significant efforts in improving BVOC estimates, the errors in emission inventories remain a concern. Since BVOC emissions are particularly sensitive to the available photosynthetically active radiation (PAR), model errors in PAR result in large errors in emission estimates. Thus, utilization of satellite observations to estimate PAR can help in reducing emission uncertainties. Satellite-based PAR estimates rely on the technique used to derive insolation from satellite visible brightness measurements. In this study we evaluate several insolation products against surface pyranometer observations and offer a bias correction to generate a more accurate PAR product. The improved PAR product is then used in biogenic emission estimates. The improved biogenic emission estimates are compared to the emission inventories over Texas and used in air quality simulation over the period of August-September 2013 (NASA's Discover-AQ field campaign). A series of sensitivity simulations will be performed and evaluated against Discover-AQ observations to test the impact of satellite-derived PAR on air quality simulations.

  8. Analyse diallele du rendement graines par hectare et du poids de ...

    African Journals Online (AJOL)

    L'analyse de variance associée aux aptitudes générales et spécifiques à la combinaison (AGC, ASC) et aux effets réciproques (ERC) montre que des effets génétiques de type additif sont à l'origine de la variabilité génétique totale pour les caractères étudiés. Ce travail a permis l'étude de l'hérédité quantitative des deux ...

  9. Usages des technologies par les élèves professeurs : analyse à partir de questionnaires

    Directory of Open Access Journals (Sweden)

    Philippe Le Borgne

    2005-01-01

    Full Text Available Cet article étudie l’appropriation et les usages des technologies chez les élèves professeurs français. La méthodologie utilisée est basée sur l’analyse de réponses à des questionnaires soumis aux professeurs stagiaires de quatre instituts universitaires de formation des maîtres. Les résultats obtenus tendent à prouver que les enseignants en formation sont aujourd’hui bien équipés et disposent de compétences réelles dans le cadre des usages personnels et professionnels en dehors de la classe. Cependant, il apparaît que ces compétences sont peu mobilisées dans le cadre des usages en classe.

  10. Safety balance: Analysis of safety systems; Bilans de surete: analyse par les organismes de surete

    Energy Technology Data Exchange (ETDEWEB)

    Delage, M; Giroux, C

    1990-12-01

    Safety analysis, and particularly analysis of exploitation of NPPs is constantly affected by EDF and by the safety authorities and their methodologies. Periodic safety reports ensure that important issues are not missed on daily basis, that incidents are identified and that relevant actions are undertaken. French safety analysis method consists of three principal steps. First type of safety balance is analyzed at the normal start-up phase for each unit including the final safety report. This enables analysis of behaviour of units ten years after their licensing. Second type is periodic operational safety analysis performed during a few years. Finally, the third step consists of safety analysis of the oldest units with the aim to improve the safety standards. The three steps of safety analysis are described in this presentation in detail with the aim to present the objectives and principles. Examples of most recent exercises are included in order to illustrate the importance of such analyses.

  11. Active zone proteins are transported via distinct mechanisms regulated by Par-1 kinase.

    Directory of Open Access Journals (Sweden)

    Kara R Barber

    2017-02-01

    Full Text Available Disruption of synapses underlies a plethora of neurodevelopmental and neurodegenerative disease. Presynaptic specialization called the active zone plays a critical role in the communication with postsynaptic neuron. While the role of many proteins at the active zones in synaptic communication is relatively well studied, very little is known about how these proteins are transported to the synapses. For example, are there distinct mechanisms for the transport of active zone components or are they all transported in the same transport vesicle? Is active zone protein transport regulated? In this report we show that overexpression of Par-1/MARK kinase, a protein whose misregulation has been implicated in Autism spectrum disorders (ASDs and neurodegenerative disorders, lead to a specific block in the transport of an active zone protein component- Bruchpilot at Drosophila neuromuscular junctions. Consistent with a block in axonal transport, we find a decrease in number of active zones and reduced neurotransmission in flies overexpressing Par-1 kinase. Interestingly, we find that Par-1 acts independently of Tau-one of the most well studied substrates of Par-1, revealing a presynaptic function for Par-1 that is independent of Tau. Thus, our study strongly suggests that there are distinct mechanisms that transport components of active zones and that they are tightly regulated.

  12. Protease activated receptors (PARS) mediation in gyroxin biological activity; Mediacao dos receptores ativados por proteases (PARs) em atividades biologicas da giroxina

    Energy Technology Data Exchange (ETDEWEB)

    Silva, Jose Alberto Alves da

    2009-07-01

    Gyroxin is a serine protease enzyme from the South American rattlesnake (Crotalus durissus terrificus) venom; it is only partially characterized and has multiple activities. Gyroxin induces blood coagulation, blood pressure decrease and a neurotoxic behavior named barrel rotation. The mechanisms involved in this neurotoxic activity are not known. Whereas gyroxin is a member of enzymes with high potential to become a new drug with clinical applications such as thrombin, batroxobin, ancrod, tripsyn and kalicrein, it is important to find out how gyroxin works. The analysis on agarose gel electrophoresis and circular dichroism confirmed the molecules' integrity and purity. The gyroxin intravenous administration in mice proved its neurotoxicity (barrel rotation). In vivo studies employing intravital microscopy proved that gyroxin induces vasodilation with the participation of protease activated receptors (PARs), nitric oxide and Na+K+ATPase. The leukocytes' adherence and rolling counting indicated that gyroxin has no pro inflammatory activity. Gyroxin induced platelet aggregation, which was blocked by inhibitors of PAR1 and PAR4 receptors (SCH 79797 and tcY-NH{sub 2}, respectively). Finally, it was proved that the gyroxin temporarily alter the permeability of the blood brain barrier (BBB). Our study has shown that both the protease-activated receptors and nitric oxide are mediators involved in the biological activities of gyroxin. (author)

  13. Isotope analysis of uranium by optical spectroscopy; Analyse isotopique de I'uranium par spectroscopie optique

    Energy Technology Data Exchange (ETDEWEB)

    Gerstenkorn, S [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

    1958-07-01

    Isotope analysis of uranium is made by means of hollow cathode lamp and Fabry-Perot photoelectric spectrometer. When using the line U I 5027 A, this method allows to determine isotopic concentrations in {sup 235}U down to 0,1 per cent. The relative precision is about 2 per cent for amounts of {sup 235}U over 1 per cent. For weaker amounts this line would allow relative measurements of better precision when using standard mixtures. (author) [French] L'analyse isotopique de l'uranium est effectuee a l'aide d'une lampe a cathode creuse et du spectrometre Fabry-Perot photo-electrique. On utilise la raie U I 5027 A. Cette methode permet de doser des melanges isotopiques dont la teneur en {sup 235}U, peut descendre jusqu'a 0,1 pour cent. La precision relative est de l'ordre de 2 pour cent pour des teneurs en {sup 235}U superieures a 1 pour cent. Pour des teneurs plus faibles cette raie {lambda} = 5027 A permettrait des mesures relatives de meilleure precision, en utilisant des melanges prealablement doses. (auteur)

  14. Analytical methods used by the geochemical section: water; Methodes d'analyses utilisees par la section de geochimie: les eaux

    Energy Technology Data Exchange (ETDEWEB)

    Berthollet, P; Cavalier, G [Commissariat a l' Energie Atomique, Fontenay-aux-Roses (France). Centre d' Etudes Nucleaires

    1971-07-01

    The authors describe the analytical methods used by the C.E.A. Geochemical Section to determine the chemical composition of natural waters encountered during the prospecting of uraniferous deposits or in the course of mining operations. Because of the diversity of the samples and the different items of information requested, methods were selected and adapted to answer to the demands peculiar to mining research. Methods and know-how concerning the quantitative analysis of natural water to find out the concentration of the following chemicals are reviewed: carbonates and bicarbonates, calcium, magnesium, chlorides, sodium and potassium, sulfates, nitrates, silica, phosphates, iron, manganese, aluminium, fluorides, dissolved oxygen, CO{sub 2}, SH{sub 2} and sulphur, and uranium. (authors) [French] Les auteurs decrivent les methodes d'analyses utilisees par la Section de Geochimie du C.E.A., pour la determination de la composition chimique des eaux naturelles rencontrees au cours de prospections de gites uraniferes ou de travaux miniers. La diversite des echantillons et les differents renseignements demandes a l'analyse les ont conduit a selectionner et a adapter des methodes afin qu'elles repondent aux exigences particulieres de la recherche miniere. Les methodes concernant le dosage dans les eaux superficielles et eaux souterraines des elements qui suivent sont presentees : carbonates and bicarbonates, calcium, magnesium, chlorures, sodium et potassium, sulfates, nitrates, silice, phosphates, fer ferreus et ferrique, manganese, aluminium, fluorures, oxygene dissous, CO{sub 2} libre, SH{sub 2} et soufre total, et uranium. (auteurs)

  15. Elevated soluble urokinase plasminogen activator receptor (suPAR) predicts mortality in Staphylococcus aureus bacteremia

    DEFF Research Database (Denmark)

    Mölkänen, T; Ruotsalainen, E; Thorball, C W

    2011-01-01

    The soluble form of urokinase-type plasminogen activator receptor (suPAR) is a new inflammatory marker. High suPAR levels have been shown to associate with mortality in cancer and in chronic infections like HIV and tuberculosis, but reports on the role of suPAR in acute bacteremic infections...... are scarce. To elucidate the role of suPAR in a common bacteremic infection, the serum suPAR levels in 59 patients with Staphylococcus aureus bacteremia (SAB) were measured using the suPARnostic ELISA assay and associations to 1-month mortality and with deep infection focus were analyzed. On day three, after...... the first positive blood culture for S. aureus, suPAR levels were higher in 19 fatalities (median 12.3; range 5.7-64.6 ng/mL) than in 40 survivors (median 8.4; range 3.7-17.6 ng/mL, p = 0.002). This difference persisted for 10 days. The presence of deep infection focus was not associated with elevated su...

  16. Effect of a physical activity intervention on suPAR levels

    DEFF Research Database (Denmark)

    Rohde, Christopher; Polcwiartek, Christoffer; Andersen, Eivind

    2018-01-01

    OBJECTIVES: Soluble urokinase-type plasminogen activator receptor (suPAR) is a novel inflammatory marker, associated with lifestyle diseases and mortality risk. No studies have investigated whether physical activity may reduce suPAR levels using a randomized controlled design. DESIGN AND METHODS......: suPAR and C-reactive protein (CRP) levels were determined in blood samples from a previous randomized controlled trial with Pakistani immigrants in Norway, 2008. The study included physically inactive men that were randomized to an intervention group (supervised group exercises) or a control group...... and followed for 5 months. A linear regression model was used and adjusted for age, inactivity level at baseline, and mean difference in CRP levels. RESULTS: Overall, 80 and 53 participants were included in the intervention and control group, respectively. Obesity and smoking were associated with higher su...

  17. MARK/Par1 Kinase Is Activated Downstream of NMDA Receptors through a PKA-Dependent Mechanism.

    Directory of Open Access Journals (Sweden)

    Laura P Bernard

    Full Text Available The Par1 kinases, also known as microtubule affinity-regulating kinases (MARKs, are important for the establishment of cell polarity from worms to mammals. Dysregulation of these kinases has been implicated in autism, Alzheimer's disease and cancer. Despite their important function in health and disease, it has been unclear how the activity of MARK/Par1 is regulated by signals from cell surface receptors. Here we show that MARK/Par1 is activated downstream of NMDA receptors in primary hippocampal neurons. Further, we show that this activation is dependent on protein kinase A (PKA, through the phosphorylation of Ser431 of Par4/LKB1, the major upstream kinase of MARK/Par1. Together, our data reveal a novel mechanism by which MARK/Par1 is activated at the neuronal synapse.

  18. Attempt of absolute analysis with spark source mass spectrometry; Essai d'analyse absolue par spectrometrie de masse a etincelles

    Energy Technology Data Exchange (ETDEWEB)

    Desjardins, M [Commissariat a l' Energie Atomique, Grenoble (France). Centre d' Etudes Nucleaires

    1964-10-01

    possibilite d'effectuer une analyse absolue par spectrometrie de masse.

  19. uPA/uPAR system activation drives a glycolytic phenotype in melanoma cells.

    Science.gov (United States)

    Laurenzana, Anna; Chillà, Anastasia; Luciani, Cristina; Peppicelli, Silvia; Biagioni, Alessio; Bianchini, Francesca; Tenedini, Elena; Torre, Eugenio; Mocali, Alessandra; Calorini, Lido; Margheri, Francesca; Fibbi, Gabriella; Del Rosso, Mario

    2017-09-15

    In this manuscript, we show the involvement of the uPA/uPAR system in the regulation of aerobic glycolysis of melanoma cells. uPAR over-expression in human melanoma cells controls an invasive and glycolytic phenotype in normoxic conditions. uPAR down-regulation by siRNA or its uncoupling from integrins, and hence from integrin-linked tyrosine kinase receptors (IL-TKRs), by an antagonist peptide induced a striking inhibition of the PI3K/AKT/mTOR/HIF1α pathway, resulting into impairment of glucose uptake, decrease of several glycolytic enzymes and of PKM2, a checkpoint that controls metabolism of cancer cells. Further, binding of uPA to uPAR regulates expression of molecules that govern cell invasion, including extracellular matrix metallo-proteinases inducer (EMPPRIN) and enolase, a glycolytyc enzyme that also serves as a plasminogen receptor, thus providing a common denominator between tumor metabolism and phenotypic invasive features. Such effects depend on the α5β1-integrin-mediated uPAR connection with EGFR in melanoma cells with engagement of the PI3K-mTOR-HIFα pathway. HIF-1α trans-activates genes whose products mediate tumor invasion and glycolysis, thus providing the common denominator between melanoma metabolism and its invasive features. These findings unveil a unrecognized interaction between the invasion-related uPAR and IL-TKRs in the control of glycolysis and disclose a new pharmacological target (i.e., uPAR/IL-TKRs axis) for the therapy of melanoma. © 2017 UICC.

  20. Estuarine morphometry governs optically active substances, Kd(PAR) and beam attenuation

    DEFF Research Database (Denmark)

    Lund-Hansen, L. C.; Nielsen, J. M.; Blüthgen, J.

    2013-01-01

    estuaries using OACs as input parameters. It is concluded that there are no large differences in OAC concentrations between the two estuaries. The spatial distributions of OACs and optical properties were significantly different and governed by the estuary morphometry, i.e. a power distribution......°N) at high discharges. The major difference was the spatial distribution of the optical properties against distance, best described by significant power functions in the ria, compared to significant linear functions in the coastal plain. It was hypothesized that estuarine morphometry could explain...... this spatial distribution. Partition and multiple regression analyses showed that Chl-a governed Kd(PAR) and beam attenuation coefficient in both estuaries. Significant, high correlations were obtained by multiple regression analyses in the estimation of Kd(PAR) and beam attenuation coefficients in the two...

  1. mTORC2 activation is regulated by the urokinase receptor (uPAR) in bladder cancer.

    Science.gov (United States)

    Hau, Andrew M; Leivo, Mariah Z; Gilder, Andrew S; Hu, Jing-Jing; Gonias, Steven L; Hansel, Donna E

    2017-01-01

    Mammalian target of rapamycin complex 2 (mTORC2) has been identified as a major regulator of bladder cancer cell migration and invasion. Upstream pathways that mediate mTORC2 activation remain poorly defined. Urokinase-type plasminogen activator receptor (uPAR) is a GPI-anchored membrane protein and known activator of cell-signaling. We identified increased uPAR expression in 94% of invasive human bladder cancers and in 54-71% of non-invasive bladder cancers, depending on grade. Normal urothelium was uPAR-immunonegative. Analysis of publicly available datasets identified uPAR gene amplification or mRNA upregulation in a subset of bladder cancer patients with reduced overall survival. Using biochemical approaches, we showed that uPAR activates mTORC2 in bladder cancer cells. Highly invasive bladder cancer cell lines, including T24, J82 and UM-UC-3 cells, showed increased uPAR mRNA expression and protein levels compared with the less aggressive cell lines, UROtsa and RT4. uPAR gene-silencing significantly reduced phosphorylation of Serine-473 in Akt, an mTORC2 target. uPAR gene-silencing also reduced bladder cancer cell migration and Matrigel invasion. S473 phosphorylation was observed by immunohistochemistry in human bladder cancers only when the tumors expressed high levels of uPAR. S473 phosphorylation was not controlled by uPAR in bladder cancer cell lines that are PTEN-negative; however, this result probably did not reflect altered mTORC2 regulation. Instead, PTEN deficiency de-repressed alternative kinases that phosphorylate S473. Our results suggest that uPAR and mTORC2 are components of a single cell-signaling pathway. Targeting uPAR or mTORC2 may be beneficial in patients with bladder cancer. Copyright © 2016. Published by Elsevier Inc.

  2. Screening of phytochemicals against protease activated receptor 1 (PAR1), a promising target for cancer.

    Science.gov (United States)

    Kakarala, Kavita Kumari; Jamil, Kaiser

    2015-02-01

    Drug resistance and drug-associated toxicity are the primary causes for withdrawal of many drugs, although patient recovery is satisfactory in many instances. Interestingly, the use of phytochemicals in the treatment of cancer as an alternative to synthetic drugs comes with a host of advantages; minimum side effects, good human absorption and low toxicity to normal cells. Protease activated receptor 1 (PAR1) has been established as a promising target in many diseases including various cancers. Strong evidences suggest its role in metastasis also. There are no natural compounds known to inhibit its activity, so we aimed to identify phytochemicals with antagonist activity against PAR1. We screened phytochemicals from Naturally Occurring Plant-based Anticancer Compound-Activity-Target database (NPACT, http://crdd.osdd.net/raghava/npact/ ) against PAR1 using virtual screening workflow of Schrödinger software. It analyzes pharmaceutically relevant properties using Qikprop and calculates binding energy using Glide at three accuracy levels (high-throughput virtual screening, standard precision and extra precision). Our study led to the identification of phytochemicals, which showed interaction with at least one experimentally determined active site residue of PAR1, showed no violations to Lipinski's rule of five along with predicted high human absorption. Furthermore, structural interaction fingerprint analysis indicated that the residues H255, D256, E260, S344, V257, L258, L262, Y337 and S344 may play an important role in the hydrogen bond interactions of the phytochemicals screened. Of these residues, H255 and L258 residues were experimentally proved to be important for antagonist binding. The residues Y183, L237, L258, L262, F271, L332, L333, Y337, L340, A349, Y350, A352, and Y353 showed maximum hydrophobic interactions with the phytochemicals screened. The results of this work suggest that phytochemicals Reissantins D, 24,25-dihydro-27-desoxywithaferin A, Isoguaiacin

  3. Activation analyses for different fusion structural alloys

    International Nuclear Information System (INIS)

    Attaya, H.; Smith, D.

    1991-01-01

    The leading candidate structural materials, viz., the vanadium alloys, the nickel or the manganese stabilized austenitic steels, and the ferritic steels, are analysed in terms of their induced activation in the TPSS fusion power reactor. The TPSS reactor has 1950 MW fusion power and inboard and outboard average neutron wall loading of 3.75 and 5.35 MW/m 2 respectively. The results shows that, after one year of continuous operation, the vanadium alloys have the least radioactivity at reactor shutdown. The maximum difference between the induced radioactivity in the vanadium alloys and in the other iron-based alloys occurs at about 10 years after reactor shutdown. At this time, the total reactor radioactivity, using the vanadium alloys, is about two orders of magnitude less than the total reactor radioactivity utilizing any other alloy. The difference is even larger in the first wall, the FW-vanadium activation is 3 orders of magnitude less than other alloys' FW activation. 2 refs., 7 figs

  4. Urokinase-type plasminogen activator receptor (uPAR), tissue factor (TF) and epidermal growth factor receptor (EGFR)

    DEFF Research Database (Denmark)

    Christensen, Anders; Kiss, Katalin; Lelkaitis, Giedrius

    2017-01-01

    Background: Tumor-specific biomarkers are a prerequisite for the development of targeted imaging and therapy in oral squamous cell carcinoma (OSCC). urokinase-type Plasminogen Activator Receptor (uPAR), Tissue Factor (TF) and Epidermal Growth Factor Receptor (EGFR) are three biomarkers that exhib...... with a reduced survival. uPAR seems to be a prognostic biomarker in oral cancer....

  5. Incorporating GOES Satellite Photosynthetically Active Radiation (PAR) Retrievals to Improve Biogenic Emission Estimates in Texas

    Science.gov (United States)

    Zhang, Rui; White, Andrew T.; Pour Biazar, Arastoo; McNider, Richard T.; Cohan, Daniel S.

    2018-01-01

    This study examines the influence of insolation and cloud retrieval products from the Geostationary Operational Environmental Satellite (GOES) system on biogenic emission estimates and ozone simulations in Texas. Compared to surface pyranometer observations, satellite-retrieved insolation and photosynthetically active radiation (PAR) values tend to systematically correct the overestimation of downwelling shortwave radiation in the Weather Research and Forecasting (WRF) model. The correlation coefficient increases from 0.93 to 0.97, and the normalized mean error decreases from 36% to 21%. The isoprene and monoterpene emissions estimated by the Model of Emissions of Gases and Aerosols from Nature are on average 20% and 5% less, respectively, when PAR from the direct satellite retrieval is used rather than the control WRF run. The reduction in biogenic emission rates using satellite PAR reduced the predicted maximum daily 8 h ozone concentration by up to 5.3 ppbV over the Dallas-Fort Worth (DFW) region on some days. However, episode average ozone response is less sensitive, with a 0.6 ppbV decrease near DFW and 0.3 ppbV increase over East Texas. The systematic overestimation of isoprene concentrations in a WRF control case is partially corrected by using satellite PAR, which observes more clouds than are simulated by WRF. Further, assimilation of GOES-derived cloud fields in WRF improved CAMx model performance for ground-level ozone over Texas. Additionally, it was found that using satellite PAR improved the model's ability to replicate the spatial pattern of satellite-derived formaldehyde columns and aircraft-observed vertical profiles of isoprene.

  6. Protease-activated receptor 2 (PAR2) is upregulated by Acanthamoeba plasminogen activator (aPA) and induces proinflammatory cytokine in human corneal epithelial cells.

    Science.gov (United States)

    Tripathi, Trivendra; Abdi, Mahshid; Alizadeh, Hassan

    2014-05-29

    Acanthamoeba plasminogen activator (aPA) is a serine protease elaborated by Acanthamoeba trophozoites that facilitates the invasion of trophozoites to the host and contributes to the pathogenesis of Acanthamoeba keratitis (AK). The aim of this study was to explore if aPA stimulates proinflammatory cytokine in human corneal epithelial (HCE) cells via the protease-activated receptors (PARs) pathway. Acanthamoeba castellanii trophozoites were grown in peptone-yeast extract glucose for 7 days, and the supernatants were collected and centrifuged. The aPA was purified using the fast protein liquid chromatography system, and aPA activity was determined by zymography assays. Human corneal epithelial cells were incubated with or without aPA (100 μg/mL), PAR1 agonists (thrombin, 10 μM; TRAP-6, 10 μM), and PAR2 agonists (SLIGRL-NH2, 100 μM; AC 55541, 10 μM) for 24 and 48 hours. Inhibition of PAR1 and PAR2 involved preincubating the HCE cells for 1 hour with the antagonist of PAR1 (SCH 79797, 60 μM) and PAR2 (FSLLRY-NH2, 100 μM) with or without aPA. Human corneal epithelial cells also were preincubated with PAR1 and PAR2 antagonists and then incubated with or without PAR1 agonists (thrombin and TRAP-6) and PAR2 agonists (SLIGRL-NH2 and AC 55541). Expression of PAR1 and PAR2 was examined by quantitative RT-PCR (qRT-PCR), flow cytometry, and immunocytochemistry. Interleukin-8 expression was quantified by qRT-PCR and ELISA. Human corneal epithelial cells constitutively expressed PAR1 and PAR2 mRNA. Acanthamoeba plasminogen activator and PAR2 agonists significantly upregulated PAR2 mRNA expression (1- and 2-fold, respectively) (P aPA, and PAR2 agonists induced PAR2 mRNA expression in HCE cells (P aPA, significantly upregulated PAR1 mRNA expression, which was significantly inhibited by PAR1 antagonist in HCE cells. Acanthamoeba plasminogen activator and PAR2 agonists stimulated IL-8 mRNA expression and protein production, which is significantly diminished by PAR2 antagonist

  7. Estimation of the fraction of absorbed photosynthetically active radiation (fPAR) in maize canopies using LiDAR data and hyperspectral imagery.

    Science.gov (United States)

    Qin, Haiming; Wang, Cheng; Zhao, Kaiguang; Xi, Xiaohuan

    2018-01-01

    Accurate estimation of the fraction of absorbed photosynthetically active radiation (fPAR) for maize canopies are important for maize growth monitoring and yield estimation. The goal of this study is to explore the potential of using airborne LiDAR and hyperspectral data to better estimate maize fPAR. This study focuses on estimating maize fPAR from (1) height and coverage metrics derived from airborne LiDAR point cloud data; (2) vegetation indices derived from hyperspectral imagery; and (3) a combination of these metrics. Pearson correlation analyses were conducted to evaluate the relationships among LiDAR metrics, hyperspectral metrics, and field-measured fPAR values. Then, multiple linear regression (MLR) models were developed using these metrics. Results showed that (1) LiDAR height and coverage metrics provided good explanatory power (i.e., R2 = 0.81); (2) hyperspectral vegetation indices provided moderate interpretability (i.e., R2 = 0.50); and (3) the combination of LiDAR metrics and hyperspectral metrics improved the LiDAR model (i.e., R2 = 0.88). These results indicate that LiDAR model seems to offer a reliable method for estimating maize fPAR at a high spatial resolution and it can be used for farmland management. Combining LiDAR and hyperspectral metrics led to better performance of maize fPAR estimation than LiDAR or hyperspectral metrics alone, which means that maize fPAR retrieval can benefit from the complementary nature of LiDAR-detected canopy structure characteristics and hyperspectral-captured vegetation spectral information.

  8. Comprendre le risque routier professionnel par l’analyse de l’activité : l’exemple des conducteurs de travaux Understanding occupational road risk by analyzing the activity: The example of project and site supervisors Comprender el riesgo laboral en carreteras a través del análisis de la actividad. El ejemplo de los conductores de trabajos

    Directory of Open Access Journals (Sweden)

    Justine Forrierre

    2010-05-01

    Full Text Available Cet article présente les résultats d’une recherche portant sur le risque routier professionnel rencontré par des travailleurs dont la conduite n’est pas le métier mais qui se déplacent fréquemment : les conducteurs de travaux de chantiers de travaux publics. En adoptant le point de vue de l’activité, nous montrons que la compréhension et la prévention de ce risque nécessitent de connaître et de comprendre l’activité de travail. En effet, l’activité de conduite est une activité de travail et le risque qui lui est lié est un risque au travail. Pour prévenir ce dernier, il est donc nécessaire de dépasser la simple approche comportementale de l’activité de conduite et de prendre en considération l’ensemble des déterminants de l’activité de travail qui font que le conducteur de travaux se déplace et prend des risques sur la route. Nous retiendrons que les aspects organisationnels (prescription, organisation de la production, politique de ressources humaines, matériel (véhicule, outils de communication et interentreprises (relationnel client affectent l’activité de conduite et que la prévention du risque routier nécessite donc une approche globale (aux niveaux individuel et collectif, dans l’entreprise et avec les entreprises extérieures qui touche à ces différents domaines.This paper presents the results of a study on the occupational road risk of workers for whom driving is not their job but who drive frequently: Public Works site supervisors. Using the activity’s theoretical framework, we show that in order to understand and prevent this risk, the work activity needs to be known and understood. In fact, driving is a work activity and the related risk is an occupational risk. To prevent the latter, it is necessary to go beyond the simple behavioural driving activity approach and consider all the determinants of the work activity that cause the work supervisor to drive and take risks on the road

  9. Activated protein C (APC) can increase bone anabolism via a protease-activated receptor (PAR)1/2 dependent mechanism.

    Science.gov (United States)

    Shen, Kaitlin; Murphy, Ciara M; Chan, Ben; Kolind, Mille; Cheng, Tegan L; Mikulec, Kathy; Peacock, Lauren; Xue, Meilang; Park, Sang-Youel; Little, David G; Jackson, Chris J; Schindeler, Aaron

    2014-12-01

    Activated Protein C (APC) is an anticoagulant with strong cytoprotective properties that has been shown to promote wound healing. In this study APC was investigated for its potential orthopedic application using a Bone Morphogenetic Protein 2 (rhBMP-2) induced ectopic bone formation model. Local co-administration of 10 µg rhBMP-2 with 10 µg or 25 µg APC increased bone volume at 3 weeks by 32% (N.S.) and 74% (pAPC are largely mediated by its receptors endothelial protein C receptor (EPCR) and protease-activated receptors (PARs). Cultured pre-osteoblasts and bone nodule tissue sections were shown to express PAR1/2 and EPCR. When pre-osteoblasts were treated with APC, cell viability and phosphorylation of ERK1/2, Akt, and p38 were increased. Inhibition with PAR1 and sometimes PAR2 antagonists, but not with EPCR blocking antibodies, ameliorated the effects of APC on cell viability and kinase phosphorylation. These data indicate that APC can affect osteoblast viability and signaling, and may have in vivo applications with rhBMP-2 for bone repair. © 2014 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.

  10. Quantitative analysis of fission products by {gamma} spectrography; Analyse quantitative des produits de fission par spectrographie {gamma}

    Energy Technology Data Exchange (ETDEWEB)

    Malet, G

    1962-07-01

    The activity of the fission products present in treated solutions of irradiated fuels is given as a function of the time of cooling and of the irradiation time. The variation of the ratio ({sup 144}Ce + {sup 144}Pr activity/{sup 137}Cs activity) as a function of these same parameters is also given. From these results a method is deduced giving the 'age' of the solution analyzed. By {gamma}-scintillation spectrography it was possible to estimate the following elements individually: {sup 141}Ce, {sup 144}Ce + {sup 144}Pr, {sup 103}Ru, {sup 106}Ru + {sup 106}Rh, {sup 137}Cs, {sup 95}Zr + {sup 95}Nb. Yield curves are given for the case of a single emitter. Of the various existing methods, that of the least squares was used for the quantitative analysis of the afore-mentioned fission products. The accuracy attained varies from 3 to 10%. (author) [French] L'activite des produits de fission presents dans les solutions de traitement de combustibles irradies est donnee en fonction du temps de refroidissement et du temps d'irradiation. On etudie de plus la variation du rapport Activite du {sup 144}Ce + {sup 144}Pr /Activite du {sup 137}Cs en fonction de ces memes parametres. De ces resultats, on deduit une methode donnant l'age de la solution analysee. La spectrographie {gamma} a scintillation a permis le dosage individuel des produits suivants: {sup 141}Ce, {sup 144}Ce + {sup 144}Pr, {sup 103}Ru, {sup 106}Ru + {sup 106}Rh, {sup 137}Cs, {sup 95}Zr + {sup 95}Nb. Des courbes de rendement sont donnees dans le cas d'un emetteur unique. Des differentes methodes existantes, la methode des moindres carres a ete employee pour l'analyse quantitative des produits de fission precites. La precision obtenue varie entre 3 et 10 pour cent. (auteur)

  11. [Roles of protease-activated receptor-2 (PAR-2), a G protein-coupled receptor, in modulation of exocrine gland functions].

    Science.gov (United States)

    Nishikawa, Hiroyuki

    2006-07-01

    Protease-activated receptor-2 (PAR-2), a G protein-coupled receptor, is activated by proteolytic unmasking of the N-terminal extracellular tethered ligand that presumably binds to the extracellular loop 2 of the receptor itself. PAR-2 is widely distributed in the mammalian body and plays various roles in biological events in the cardiovascular, respiratory, alimentary, and central neurons systems. PAR-2-activating peptides administered systemically to mice and rats trigger prompt salivation in vivo. In an in vitro study, PAR-2 agonists including the endogenous PAR-2 activator trypsin induce secretion of amylase and mucin from isolated rat parotid glands and sublingual glands, respectively. PAR-2-activating peptides administered systemically also modulate pancreatic exocrine secretion in vivo as well as in vitro. In the gastric mucosa, PAR-2 stimulation enhances secretion of mucus and pepsinogen and suppresses acid secretion. Tear secretion can also be caused by PAR-2-related peptides in PAR-2-dependent and -independent manners. PAR-2 thus plays a general or key role in the regulation of exocrine secretion. This review focuses on the physiologic and/or pathophysiologic roles of PAR-2 in glandular exocrine secretion. The possibility of PAR-2 as a target for drug development is also discussed.

  12. Soluble urokinase plasminogen activator receptor (suPAR) in acute care

    DEFF Research Database (Denmark)

    Rasmussen, Line Jee Hartmann; Ladelund, Steen; Haupt, Thomas Huneck

    2016-01-01

    for age, sex, Charlson score and C reactive protein. Area under the curve for receiver operating characteristics curve analysis of suPAR for 30-day mortality was 0.84 (95% CI 0.81 to 0.86). Furthermore, in the entire cohort, women had slightly higher suPAR compared with men, and suPAR was associated...

  13. Proteinase-Activated Receptor 1 (PAR1 regulates leukemic stem cell functions.

    Directory of Open Access Journals (Sweden)

    Nicole Bäumer

    Full Text Available External signals that are mediated by specific receptors determine stem cell fate. The thrombin receptor PAR1 plays an important role in haemostasis, thrombosis and vascular biology, but also in tumor biology and angiogenesis. Its expression and function in hematopoietic stem cells is largely unknown. Here, we analyzed expression and function of PAR1 in primary hematopoietic cells and their leukemic counterparts. AML patients' blast cells expressed much lower levels of PAR1 mRNA and protein than CD34+ progenitor cells. Constitutive Par1-deficiency in adult mice did not affect engraftment or stem cell potential of hematopoietic cells. To model an AML with Par1-deficiency, we retrovirally introduced the oncogene MLL-AF9 in wild type and Par1-/- hematopoietic progenitor cells. Par1-deficiency did not alter initial leukemia development. However, the loss of Par1 enhanced leukemic stem cell function in vitro and in vivo. Re-expression of PAR1 in Par1-/- leukemic stem cells delayed leukemogenesis in vivo. These data indicate that Par1 contributes to leukemic stem cell maintenance.

  14. Proteinase-Activated Receptor 1 (PAR1) regulates leukemic stem cell functions.

    Science.gov (United States)

    Bäumer, Nicole; Krause, Annika; Köhler, Gabriele; Lettermann, Stephanie; Evers, Georg; Hascher, Antje; Bäumer, Sebastian; Berdel, Wolfgang E; Müller-Tidow, Carsten; Tickenbrock, Lara

    2014-01-01

    External signals that are mediated by specific receptors determine stem cell fate. The thrombin receptor PAR1 plays an important role in haemostasis, thrombosis and vascular biology, but also in tumor biology and angiogenesis. Its expression and function in hematopoietic stem cells is largely unknown. Here, we analyzed expression and function of PAR1 in primary hematopoietic cells and their leukemic counterparts. AML patients' blast cells expressed much lower levels of PAR1 mRNA and protein than CD34+ progenitor cells. Constitutive Par1-deficiency in adult mice did not affect engraftment or stem cell potential of hematopoietic cells. To model an AML with Par1-deficiency, we retrovirally introduced the oncogene MLL-AF9 in wild type and Par1-/- hematopoietic progenitor cells. Par1-deficiency did not alter initial leukemia development. However, the loss of Par1 enhanced leukemic stem cell function in vitro and in vivo. Re-expression of PAR1 in Par1-/- leukemic stem cells delayed leukemogenesis in vivo. These data indicate that Par1 contributes to leukemic stem cell maintenance.

  15. Increased Soluble Urokinase-Type Plasminogen Activator Receptor (suPAR Levels in Plasma of Suicide Attempters.

    Directory of Open Access Journals (Sweden)

    Filip Ventorp

    Full Text Available The soluble form of the urokinase receptor, suPAR, has been suggested as a novel biomarker of low-grade inflammation. Activation of the immune system has been proposed to contribute to the development of depression and suicidal behavior. In order to identify depressed and suicidal individuals who could benefit from an anti-inflammatory treatment, a reliable biomarker of low-grade inflammation is vital. This study evaluates plasma suPAR levels as a biomarker of low-grade inflammation in patients with major depressive disorder and in patients who recently attempted suicide. The plasma suPAR and an established biomarker, C reactive protein (CRP of suicide attempters (n = 54, depressed patients (n = 19 and healthy controls (n = 19 was analyzed with enzyme-linked immunosorbent assays. The biomarker attributes of sensitivity and sensibility were evaluated using ROC curve analysis. Both the depressed patients and suicide attempters had increased plasma suPAR. The levels of suPAR discriminated better between controls and suicide attempters than did CRP. In the future, plasma suPAR might be a superior prognosticator regarding outcome of treatment applying conventional antidepressants in conjunction with anti-inflammatory drugs.

  16. A cluster of aspartic residues in the extracellular loop II of PAR 4 is important for thrombin interaction and activation of platelets.

    Science.gov (United States)

    Sánchez Centellas, Daniel; Gudlur, Sushanth; Vicente-Carrillo, Alejandro; Ramström, Sofia; Lindahl, Tomas L

    2017-06-01

    Thrombin activates platelets via proteolytic cleavage of protease-activated receptors (PARs) 1 and 4. The two PARs have distinct but complementary roles. The mechanisms responsible for PAR1 activation by thrombin have been extensively studied. However, much less is known regarding thrombin activation of PAR4, especially the potential involvement of regions of PAR4 other than the N-terminal, which is bound to the catalytic site of thrombin. We have studied PAR4 in S. cerevisiae strain MMY12, an expression system in which the GPCR receptors are connected to a Lac Z reporter gene resulting in increased β-galactosidase activity. This approach was used to assess PAR4 mutants to evaluate the contribution of different aspartic residues in facilitating PAR4 activation. Furthermore, peptides mimicking parts of the PAR4 N-terminal and the second extracellular loop (ECLII) were tested for their ability to inhibit platelet activation by thrombin. Binding of these peptides to γ-thrombin was studied by monitoring the decrease in tryptophan fluorescence intensity of thrombin. We conclude that not only the N-terminal but also the electronegative aspartic residues D224, D230 and D235 (located in ECLII) are be important for PAR4 binding to thrombin. We further suggest that they play a role for the tethered ligand binding to the receptor, as mutations also affected activation in response to a PAR4-activating peptide mimicking the new N-terminal formed after cleavage. This agrees with previous results on PAR1 and thrombin binding. We suggest that the ECLII of PAR4 could be a potential target for antithrombotic drug development. Copyright © 2017 Elsevier Ltd. All rights reserved.

  17. Analyse géochimique des hydrocarbures légers par thermovaporisation Geochemical Analysis of Light Hydrocarbons by Thermovaporization

    OpenAIRE

    Jonathan D.; L'hote G.; Du Rouchet J.

    2006-01-01

    Le procédé de thermovaporisation est utilisé pour l'extraction et l'analyse des hydrocarbures à point d'ébullition situé à l'intérieur de l'intervalle correspondant aux alcanes C6 à C15; il s'applique également aux roches et aux huiles. La mise en ceuvre est simple et rapide, ce qui rend cette technique utilisable dans certains laboratoires de chantiers d'exploration. Les chromatogrammes de vapeurs montrent une succession de pics, chaque pic correspondant théoriquement à un composant défini, ...

  18. Urokinase-type plasminogen activator receptor (uPAR) as a promising new imaging target

    DEFF Research Database (Denmark)

    Persson, Morten; Kjaer, Andreas

    2013-01-01

    modalities such as optical imaging, magnetic resonance imaging, single photon emission computer tomography (SPECT) and positron emission topography (PET). In this review, we will discuss recent advances in the development of uPAR-targeted imaging ligands according to imaging modality. In addition, we...... will discuss the potential future clinical application for uPAR imaging as a new imaging biomarker....

  19. Analyse de l'évolution de la consommation de plats préparés en Belgique

    Directory of Open Access Journals (Sweden)

    Winandy, S.

    2013-01-01

    Full Text Available Trend analysis of the consumption of ready-made food in Belgium. This study aims to analyze the evolution of the consumption of ready-made food in Belgium and to discern any trends. The data used are expenditure made on convenience food from 1999 to 2009 (in euros and socio-demographic characteristics, obtained from the Household Budget Survey undertaken annually by the Federal Public Service Economy (DGSIE. Statistical analyses (simple, multiple and binary logistic regressions enable the study of consumption trends in terms of expenditure and percentage of consumers as well as the determination of consumer profiles. Convenience food is part of the eating habits of Belgian households, with more than nine out of ten households being consumers. Convenience food prepared from meat is the most frequently purchased. For all ready-made food, the variable of household size explains the most variability in consumption, followed by the variables of income and age.

  20. Analyse géochimique des hydrocarbures légers par thermovaporisation Geochemical Analysis of Light Hydrocarbons by Thermovaporization

    Directory of Open Access Journals (Sweden)

    Jonathan D.

    2006-11-01

    Full Text Available Le procédé de thermovaporisation est utilisé pour l'extraction et l'analyse des hydrocarbures à point d'ébullition situé à l'intérieur de l'intervalle correspondant aux alcanes C6 à C15; il s'applique également aux roches et aux huiles. La mise en ceuvre est simple et rapide, ce qui rend cette technique utilisable dans certains laboratoires de chantiers d'exploration. Les chromatogrammes de vapeurs montrent une succession de pics, chaque pic correspondant théoriquement à un composant défini, alcane, cyclane ou aromatique; la portion située entre les alcanes n-C, et n-C8 est très typée, mais le reste du chromatogramme apporte également des informations importantes ; l'ensemble donne une définition chimique très fine des hydrocarbures légers présents dans l'échantillon. L'exploitation de ces informations en interprétation géochimique est multiple (pouvoir pétroligène de sédiments, état de maturation, etc.. La faible masse de la prise d'essai nécessaire constitue un intérêt essentiel de la méthode. The method called thermovaporization is used to extract and analyse low boiling point hydrocarbons in the range corresponding to C6-C15; alcanes. Sediments and oils ore examined in the same way. This method is simple and fast to utilize, and for this reason it can be applied in some field exploration laboratories. Chromatograms show a sertes of peaks, each of them theoretically corresponding to a particular composant : alkane, cyclone, arcmatics. The part of the chromatogram between n-C7 n-C8 is very characteristic, but other parts also provide a great deal of information. The total chromatogram gives a very fine chemical definition of the light hydrocarbons in the sample. This information can be used for many geochemical interpretations (hydrocarbon yield potential, state of maturation, etc.. Moreover, the tiny amount of sample thot is necessary is of major interest.

  1. Migration-Velocity Analysis for Ti and Orthorhombic Background Media Analyse de vitesse par migration dans les cas de milieux environnantsTI et orthorhombiques

    Directory of Open Access Journals (Sweden)

    Ikelle L. T.

    2006-12-01

    migration instead of using directly. Il est essentiel de connaître le modèle de vitesse du milieu environnant pour effectuer la description précise du réservoir que l'on veut obtenir par imagerie et par inversion. Les méthodes conventionnelles permettant de reconstituer le modèle de vitesse de fond, telles que les méthodes de vitesse migratoire, supposent souvent une sous-surface isotrope et peuvent donner des descriptions de réservoirs manquant de précisions lorsque la sous-surface contient des formations de roches anisotropes. Nous généralisons ici le concept de vitesse migratoire en permettant à la vitesse de fond d'être transversalement isotrope (TI par rapport à l'axe vertical ou orthorhombique. Cette méthode consiste à scanner différents modèles de vitesse anisotropes à l'aide d'une migration à décalage de phases et de sélectionner des paramètres anisotropes sur la base des variations d'amplitude des résultats migrés (analyse par focalisation. Sachant que le modèle de vitesse de fond anisotrope est généralement décrit par plusieurs coefficients d'élasticité, il importe d'adopter une procédure de scannage efficace. Nous avons choisi de travailler sur des sections azimutales communes. Pour une section azimutale commune donnée, nous avons effectué une analyse continue de deux paramètres : la vitesse normale de déplacement et le paramètre anisotrope connu sous le nom d'anellipticité. Ces deux balayages nous permettent de reconstituer un modèle de vitesse azimutalement isotrope. L'opération est ensuite répétée pour différentes sections azimutales communes ; chacune d'entre elles donne un nouveau modèle de vitesse azimutalement isotrope si le support est azimutalement anisotrope. Le nombre de sections azimutales communes, et par conséquent le nombre de modèles de vitesse azimutalement isotropes nécessaires pour reconstituer un modèle de vitesse azimutalement anisotrope, dépend du type de symétries. Par exemple, il faut

  2. Some studies on the formulation of 'Khun-hnit-par-shaung' Myanmar indigenous medicine by neutron activation analysis

    International Nuclear Information System (INIS)

    Myint, U.; Than Dar Shwe

    1995-01-01

    A study on the variation of arsenic content in 'Khun-hnit-par-shaung' indigenous medicine formulated under various experimental conditions such as heating interval, sand amount and heating temperature has been carried out. An Am(Be) neutron source was used for the activation of samples and 76 As was radioactivity measured by a γ-counting technique. (author). 2 refs., 5 tabs

  3. Detailed analysis of petroleum cuts by multidimensional gas chromatography; Analyse detaillee de coupes petrolieres par chromatographie en phase gazeuse multidimensionnelle

    Energy Technology Data Exchange (ETDEWEB)

    Vendeuvre, C.

    2006-01-15

    The limitations of petroleum resources implying a better valorisation of crude oil through the optimisation of production, refinery and petrochemistry processes, as well as the environmental regulations have strengthened the necessity of more detailed characterisation of petroleum products. In order to take up this challenge, efficient analytical tools have to be developed. This work demonstrates that comprehensive two-dimensional gas chromatography (GCxGC) constitutes a major advance compared to GC owing to its improved resolution power and to the structured chromatograms indicating the polarity and the volatility of hydrocarbons. The principle of GCxGC is based on the analysis of a whole sample in two independent dimensions of separation achieved using two GC columns of different selectivities; between the two columns a modulator device samples, focuses and re-injects small portions of the effluent from the first column into the second one. Since its introduction in 1991, GCxGC has known a rapid growth and has received a wide acceptance by the analytical science community. The competitive situation has considerably evolved during this thesis with the introduction of commercial systems and the two first sessions of an international symposium dedicated to this technique (Volendam, 2003 and Atlanta, 2004). The key points of the thesis concern the development of a GCxGC prototype system using dual jets CO{sub 2} technology and a data processing program; the evaluation of a retention model allowing a rational choice of operating conditions; and the application of this technique to various and complex issues. Thus, effluents from petrochemistry, refinery or pollution areas have been analysed according to the chemical classes of hydrocarbons and to their number of carbon atoms; a new method for obtaining distillation curves for each chemical group was also presented. Furthermore, the hyphenation of GCxGC with a specific sulphur detector revealed a great interest for

  4. Improvement of spectrographic analyses by the use of a mechanical packer in the arc distillation technique; Amelioration de l'analyse spectrograpique par l'utilisation d'un tasseur mecanique dans la methode de distillation dans l'arc

    Energy Technology Data Exchange (ETDEWEB)

    Buffereau, M; Deniaud, S; Pichotin, B; Violet, R [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

    1965-07-01

    One studies improvement of spectrographic analysis by the 'carrier distillation' method with the help of a mechanical device. Experiments and advantages of such an apparatus are given (precision and reproducibility improvement, operator factor suppression). A routine apparatus (French patent no 976.493) is described. (authors) [French] On etudie l'amelioration des analyses spectrographiques par la methode de distillation avec entra eur dans l'arc au moyen d'un tasseur mecanique. On indique les experiences realisees et les avantages de l'emploi d'un tel appareil (amelioration de la precision et de la reproductibilite, suppression du facteur operateur). On decrit l'appareil de routine objet du brevet no 976.493 du 29 mai 1964. (auteurs)

  5. The determination of iodine in biological media using radioactivation analysis (1962); Dosage de l'iode dans les milieux biologiques au moyen de l'analyse par radioactivation (1962)

    Energy Technology Data Exchange (ETDEWEB)

    Comar, D [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

    1962-06-15

    The object of this study is to show that the application of radioactivation analysis to the determination of iodine in biological media makes it possible to measure iodine concentrations of the order of 0.0001 {mu}g. After a review of the chemical methods with a mention of the difficulties they present, the optimum conditions for the determination of iodine in biological liquids are given. Three methods are described: - the first consists of a chemical treatment which liberates the protein bound iodine in an inorganic form. After distillation this iodine is irradiated in a flux of thermal neutrons. The induced radioactivity is compared to that of a standard sample irradiated in the same conditions by {gamma} spectrometry. - the second method which is of more general application consists in irradiating the sample and then extracting the iodine; its induced radio-activity is then measured by {beta}-counting. - the third method measures the iodine directly in the thyroid tissue by anti-compton spectrometry. The sensitivity, the reproducibility and the accuracy are discussed. Some applications are described: determination of iodine in its various organic forms in serum, determination of iodine in urines, in food-stuffs, etc., in the thyroid tissue, etc. (author) [French] Le but de cette etude est de montrer que l'analyse par radioactivation appliquee au dosage de l'iode dans les milieux biologiques permet de mesurer des taux d'iode de l'ordie de 0,0001 {mu}g. Apres avoir rappele le principe des methodes chimiques et montre les difficultes de leur mise en oeuvre, il est etabli les conditions optima pour realiser le dosage de l'iode dans les liquides biologiques. Trois methodes sont decrites; - la premiere consiste a pratiquer un traitement chimique liberant l'iode proteique sous forme minerale. Apres distillation cet iode est irradie dans un flux de neutrons thermiques. La radioactivite induite est mesuree comparativement a celle d'un etalon traite dans les memes

  6. Contribution to the isotopic analysis of nitrogen using optical spectrometry; Contribution a l'analyse isotopique de l'azote par spectrometrie optique

    Energy Technology Data Exchange (ETDEWEB)

    Leicknam, J P [Commissariat a l' Energie Atomique, 91 - Saclay (France). Centre d' Etudes Nucleaires

    1968-07-01

    The possibility of analyzing a gaseous mixture of {sup 14}N{sub 2} and {sup 14}N-{sup 15}N by emission spectrometry is well known. However, for mixtures which are poor in nitrogen 15 (isotopic abundance less than 2 per cent), the methods proposed up till now do not give sufficiently accurate results rapidly. The method of isotopic analysis proposed here only requires a spectrometer of average resolving power; it is even possible, as we have done; to use a spectrograph with a slit replacing the photographic plate, and a photomultiplier; this transformation is very simple to carry out and the signals from the detector can then be received by a recorder. Under these conditions a measurement takes only from three to ten minutes, according to the accuracy required. The light sources are made up of electrode-less discharge lamps, excited by high frequency; the filling conditions are such that the lamps may be prepared in a few minutes, the impurities being sufficiently eliminated for the measurements not to be affected. The choice of the analytical band couple depends on the relative position of the isotopic bands and on the possible presence of traces of these impurities: amongst the transitions in the system C({sup 3}{pi}{sub u}) <-> B({sup 3}{pi}{sub g}), it is the 2 {yields} 0 transition which has the most favorable position for this isotopic analysis. The method is applied to samples of varying origins (ammonium salts, fertilizer and plants): the isotopic contents are determined with an accuracy of 3 per cent in the neighbourhood of the natural abundance, and of 1 per cent when the sample contains about 1.5 atoms of nitrogen-15 for 100 atoms of nitrogen-14. The accuracy is therefore quite sufficient for most of the biological research using the mass 15 as tracer. (author) [French] La possibilite d'analyser un melange gazeux des azotes {sup 14}N{sub 2} et {sup 14}N-{sup 15}N par spectrometrie d'emission est bien connue. Cependant, pour des melanges pauvres en azote

  7. Mapping the topographic epitope landscape on the urokinase plasminogen activator receptor (uPAR) by surface plasmon resonance and X-ray crystallography

    DEFF Research Database (Denmark)

    Zhao, Baoyu; Gandhi, Sonu; Yuan, Cai

    2015-01-01

    The urokinase-type plasminogen activator receptor (uPAR or CD87) is a glycolipid-anchored membrane protein often expressed in the microenvironment of invasive solid cancers and high levels are generally associated with poor patient prognosis (Kriegbaum et al., 2011 [1]). uPAR is organized as a dy...... of these mAbs by X-ray crystallography alone and in complex with uPAR [deposited in the PDB database as 4QTH and 4QTI, respectively]....

  8. A method for reducing memory errors in the isotopic analyses of uranium hexafluoride by mass spectrometry; Methode de reduction des erreurs de memoire dans les analyses isotopiques de l'hexafluorure d'uranium par spectrometrie de masse

    Energy Technology Data Exchange (ETDEWEB)

    Bir, R [Commissariat a l' Energie Atomique, Saclay (France).Centre d' Etudes Nucleaires

    1961-07-01

    One of the most serious causes of systematic error in isotopic analyses of uranium from UF{sub 6} is the tendency of this material to become fixed in various ways in the mass spectrometer. As a result the value indicated by the instrument is influenced by the isotopic composition of the substances previously analysed. The resulting error is called a memory error. Making use of an elementary mathematical theory, the various methods used to reduce memory errors are analysed and compared. A new method is then suggested, which reduces the memory errors to an extent where they become negligible over a wide range of {sup 235}U concentration. The method is given in full, together with examples of its application. (author) [French] Une des causes d'erreurs systematiques les plus graves dans les analyses isotopiques d'uranium a partir d'UF{sub 6} est l'aptitude de ce produit a se fixer de diverses manieres dans le spectrometre de masse. Il en resulte une influence de la composition isotopique des produits precedemment analyses sur la valeur indiquee par l'appareil. L'erreur resultante est appelee erreur de memoire. A partir d'une theorie mathematique elementaire, on analyse et on compare les differentes methodes utilisees pour reduire les erreurs de memoire. On suggere ensuite une nouvelle methode qui reduit les erreurs de memoire dans une proportion telle qu'elles deviennent negligeables dans un grand domaine de concentration en {sup 235}U. On donne le mode operatoire complet et des exemples d'application. (auteur)

  9. Measures and modelling of PAR (photosynthetically-active radiation) for the Northeast of Brazil; Medidas e modelagem da radiacao PAR (photosynthetically-active radiation) para o nordeste do Brasil

    Energy Technology Data Exchange (ETDEWEB)

    Tiba, Chigueru; Leal, Sergio da S.A. [Universidade Federal de Pernambuco (UFPE), Recife, PE (Brazil). Dept. de Energia Nuclear], e-mail: tiba@rce.neoline.com.br

    2004-07-01

    Photosynthetically active solar radiation, known by its acronym in the English language as PAR, is the principal driving force of innumerable biological and physical processes related to biomass production, such as, the evolution of vegetal covering, agricultural productivity, and countless environment aspects, among others. Unfortunately in Brazil and particularly in the Northeast of Brazil, the PAR radiation measures are not a routine part of meteorological station measures, and therefore are still rarer than solar irradiation measures. In this context, a station was installed in Recife, Pernambuco in 2003, to carry out simultaneous measures of daily solar irradiation and PAR irradiation, which permits the modelling and valuation of the relationship between these two parameters and thus makes the estimation of PAR radiation possible, where there used to be only information on solar irradiation. Three others stations are being installed, one on Fernando de Noronha-PE, another in Pesqueira-PE, and the other in Xingo-SE, which complete a group of 4 between Latitudes 8 deg and 10 deg South and Longitudes 34 deg to 38 deg West, each having differentiated Equatorial Climates: island maritime, continental maritime, sylvan (Agreste) and semi-arid. (author)

  10. suPAR

    DEFF Research Database (Denmark)

    Hodges, Gethin W; Bang, Casper N; Wachtell, Kristian

    2015-01-01

    The fundamental role of inflammation in cardiovascular disease (CVD) has prompted interest in numerous biomarkers that detect subclinical levels of inflammation. Soluble urokinase plasminogen activator receptor (suPAR) is a novel biomarker that correlates significantly with cardiovascular events ...... comprehensive review of suPAR in CVD and explore its function and usefulness in predicting cardiovascular events....

  11. Prognostic value of plasma soluble urokinase plasminogen activator receptor (suPAR) in Danish patients with recurrent epithelial ovarian cancer (REOC)

    DEFF Research Database (Denmark)

    Begum, Farah Diba; Høgdall, Estrid V S; Riisbo, Rikke

    2006-01-01

    The level of the soluble urokinase plasminogen activator receptor (suPAR) is elevated in tumour tissue from several types of cancer. This is the first study aiming to predict the prognosis for survival by the use of a pre-chemotherapeutic plasma suPAR value in 71 patients with recurrent epithelial...

  12. PAR-Q & YOU Questionnaire and cardiovascular history of elderly patients on dialysis engaged in physical activity

    Directory of Open Access Journals (Sweden)

    Jorge Enrique Moreno-Collazos

    2017-10-01

    Full Text Available Introduction: During the last few years, physical activity has become a therapeutic tool and a protective mechanism for the elderly; it reduces cardiovascular risk in patients undergoing different types of dialysis and receiving palliative care. Objective: The aim of this study was to establish the inclusion criteria for a therapeutic physical activity program through the use of the PAR-Q & YOU Questionnaire with elderly patients treated with different types of hemodialysis, and to relate it with cardiovascular risk factors. Methods: Pearson's chi-squared test was used to determine the possible association among variables, considering the medical history and discomfort caused by physical activity against the results of PAR-Q & YOU. Binary logistic regression was used with the variables in the chi-squared test. Conclusions: Through logistic regression, we found that cardiovascular history was 10.44 times more significant to establish the relevance of the PAR-Q & YOU as a basic assessment instrument for the inclusion in a physical activity program which is part of a physiotherapy-led renal rehabilitation.

  13. Galectin-3 facilitates cell motility in gastric cancer by up-regulating protease-activated receptor-1 (PAR-1 and matrix metalloproteinase-1 (MMP-1.

    Directory of Open Access Journals (Sweden)

    Seok-Jun Kim

    Full Text Available BACKGROUND: Galectin-3 is known to regulate cancer metastasis. However, the underlying mechanism has not been defined. Through the DNA microarray studies after galectin-3 silencing, we demonstrated here that galectin-3 plays a key role in up-regulating the expressions of protease-activated receptor-1 (PAR-1 and matrix metalloproteinase-1 (MMP-1 PAR-1 thereby promoting gastric cancer metastasis. METHODOLOGY/PRINCIPAL FINDINGS: We examined the expression levels of Galectin-3, PAR-1, and MMP-1 in gastric cancer patient tissues and also the effects of silencing these proteins with specific siRNAs and of over-expressing them using specific lenti-viral constructs. We also employed zebrafish embryo model for analysis of in vivo gastric cancer cell invasion. These studies demonstrated that: a galectin-3 silencing decreases the expression of PAR-1. b galectin-3 over-expression increases cell migration and invasion and this increase can be reversed by PAR-1 silencing, indicating that galectin-3 increases cell migration and invasion via PAR-1 up-regulation. c galectin-3 directly interacts with AP-1 transcriptional factor, and this complex binds to PAR-1 promoter and drives PAR-1 transcription. d galectin-3 also amplifies phospho-paxillin, a PAR-1 downstream target, by increasing MMP-1 expression. MMP-1 silencing blocks phospho-paxillin amplification and cell invasion caused by galectin-3 over-expression. e Silencing of either galectin-3, PAR-1 or MMP-1 significantly reduced cell migration into the vessels in zebrafish embryo model. f Galectin-3, PAR-1, and MMP-1 are highly expressed and co-localized in malignant tissues from gastric cancer patients. CONCLUSIONS/SIGNIFICANCE: Galectin-3 plays the key role of activating cell surface receptor through production of protease and boosts gastric cancer metastasis. Galectin-3 has the potential to serve as a useful pharmacological target for prevention of gastric cancer metastasis.

  14. UTILITY OF A WIDE SPECTRUM LIGHT METER AS AN UNDERWATER SENSOR OF PHOTOSYNTHETICALLY ACTIVE RADIATION (PAR)

    Science.gov (United States)

    The strong attenuation of infra red wavelengths (>700 nm) in coastal waters is suggestive that some instruments with broad spectral responses might be useful, inexpensive substitutes for PAR sensors in studies of estuarine plant dynamics. Wide spectrum (350-1100 nm) light intensi...

  15. Flow cytometry analysis reveals different activation profiles of thrombin- or TRAP-stimulated platelets in db/db mice. The regulatory role of PAR-3.

    Science.gov (United States)

    Kassassir, Hassan; Siewiera, Karolina; Talar, Marcin; Przygodzki, Tomasz; Watala, Cezary

    2017-06-01

    Recent studies have shown that it may be the concentration of thrombin, which is discriminative in determining of the mechanism of platelet activation via protease activated receptors (PARs). Whether the observed phenomenon of differentiated responses of mouse platelets to various thrombin concentrations in non-diabetic db/+ and diabetic db/db mice depends upon the concerted action of various PARs, remains to be established. We found elevated reactivity of platelets, as well as the enhanced PAR-3 expression in response to both the used concentrations of AYPGKF in db/db mice, as compared to db/+ heterozygotes. At low concentration of thrombin platelets from diabetic mice demonstrated hyperreactivity, reflected by higher expression of PAR-3. For higher thrombin concentration, blood platelets from db/db mice appeared hyporeactive, compared to db/+ animals, while no significant differences in PAR-3 expression were observed between diabetic and non-diabetic mice. The novel and previously unreported finding resulting from our study is that the increased expression of PAR-3 in response to either TRAP for PAR-4 or low thrombin (when PAR-4 is not the efficient thrombin receptor) may be one of the key events contributing to higher reactivity of platelets in db/db mice. Copyright © 2017 Elsevier Inc. All rights reserved.

  16. Contribution to the determination of Sb-Ag-Cu-Ga-Mo-Zn using 14 MeV neutron activation; Contribution au dosage de Sb-Ag-Cu-Ga-Mo-Zn par activation aux neutrons de 14 MeV

    Energy Technology Data Exchange (ETDEWEB)

    Crambes, M [Commissariat a l' Energie Atomique, Grenoble (France). Centre d' Etudes Nucleaires

    1966-04-01

    By using, 14 MeV, neutron irradiation it is possible to extend the field of application of neutron radio-activation analysis, in particular to the case of light elements. For, many other elements it can replace in-pile irradiation thereby making it possible, thanks to portable 14 MeV neutron generators, to carry out radio-activation analyses away from nuclear-research c e n t r e s. With a view to applying this analytical technique to routine work, we have developed some rapid chemical separation methods in order to make possible the determination of several elements which after exposure to fast neutrons, produce {beta} emitting nuclides which cannot be differentiated by a simple instrumental study, the emitted radiation being of the same type and of similar half-life the two cases. (author) [French] L'irradiation au moyen de neutrons de 14 MeV permet d'etendre le domaine d'application de l'analyse par radioactivation neutronique, en particulier aux elements legers. Cependant pour de nombreux autres elements elle peut remplacer l'irradiation en reacteur nucleaire permettant ainsi grace aux ensembles portables producteurs de neutrons de 14 MeV, l'extension de l'analyse par radioactivation a l'exterieur des centres d'etudes nucleaires. Dans le but d'appliquer cette methode d'analyse a des travaux de routine, nous avons mis au point des separations chimiques rapides, afin de permettre le dosage de quelques elements qui par irradiation aux neutrons rapides, engendrent des nucleides emetteurs {beta} qu'une simple etude instrumentale ne peut differencier en raison de l'identite de leur rayonnement et de leurs periodes radioactives trop proches. (auteur)

  17. Mass-spectrometric analysis of trace oxygen in carbon dioxide; Analyse de traces d'oxygene dans le gaz carbonique par spectrometrie de masse

    Energy Technology Data Exchange (ETDEWEB)

    Nief, G; Severin, M [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

    1961-07-01

    The mass spectrum of pure CO{sub 2} contains a peak of weight 32 whose relationship to peak at 44 varies greatly depending on the previous history of the source of ions and even during the course of an analysis. The fact that this peak is more or less proportional to the pressure and that its appearance potential is the same as that of oxygen leads us to suppose that it is produced from oxygen formed by dissociation of the carbon dioxide on the tungsten filament. A prior treatment of the ion source with acetylene reduces the ratio 32/44 to a value of about 15.10{sup -5}. This same treatment also stabilises the spectrometer's sensitivity to oxygen. Two lines of introduction enable pure carbon dioxide, the specimen to be estimated and a reference mixture of known oxygen content to be sent into the mass spectrometer in quick succession. Oxygen in the carbon dioxide in amounts ranging between 0 and 500 p.p.m. can thus be determined to an accuracy of {+-} 5 p.p.m., the analysis taking 30 minutes. (author) [French] Le spectre de masse de CO{sub 2} pur contient un pic de masse 32 dont le rapport au pic 44 varie enormement suivant l'histoire anterieure de la source d'ions et meme au cours d'une analyse. Le fait que ce pic soit grossierement proportionnel a la pression et que son potentiel d'apparition soit le meme que celui de l'oxygene permet de supposer qu'il est engendre a partir de l'oxygene produit par dissociation du gaz carbonique sur le filament de tungstene. Un conditionnement prealable de la source d'ions avec de l'acetylene reduit le rapport 32/44 a une valeur d'environ 15.10{sup -5}. Ce meme traitement stabilise egalement la sensibilite du spectrometre vis-a-vis de l'oxygene. Une double ligne d'introduction de gaz permet d'envoyer en successions rapides dans le spectrometre de masse le gaz carbonique pur, l'echantillon a doser et un melange de reference de teneur en oxygene connue. On arrive ainsi a doser l'oxygene dans le gaz carbonique dans la gamme de 0 a

  18. Urokinase-type plasminogen activator receptor (uPAR) expression is associated with T-stage and survival in urothelial carcinoma of the bladder

    DEFF Research Database (Denmark)

    Dohn, Line Hammer; Illemann, Martin; Høyer-Hansen, Gunilla

    2015-01-01

    OBJECTIVES: To evaluate the expression-and localization pattern of the urokinase-type plasminogen activator receptor (uPAR), focusing on its clinical implications in patients with urothelial neoplasia of the bladder treated with radical cystectomy. uPAR is a central molecule in tissue remodeling...... during cancer invasion and metastasis and is an established prognostic marker in cancer. The expression and localization of uPAR and its prognostic significance is only limitedly investigated in urothelial bladder neoplasia. MATERIALS AND METHODS: The expression-and localization pattern of u......PAR was investigated in formalin-fixed paraffin-embedded tumor tissue from 149 patients treated with radical cystectomy between 1988 and 2005. uPAR expression was determined by immunohistochemistry and scored as either negative or positive. Separate values were obtained for cancer cells, macrophages...

  19. Analyse de la nature constructiviste d'une activité d'apprentissage collaboratif médié par les TIC

    Directory of Open Access Journals (Sweden)

    Nancy Brousseau

    2003-10-01

    Full Text Available Abstract. In this article we examine an example of collaborative learning with ICT in secondary school science, in order to analyse its characteristics and determine if they answer criteria for an activity of constructivist nature. We retain that constructivism places the student at the centre of her learning; opposes the notion of learning as transmission of knowledge; requires an active participation of the learner who must build and reconstruct, individually and collectively, her knowledge in order to solve a problem or realise a project. In light of these criteria, we conclude that an activity such as the TACTICS project one, can be considered of a constructivist nature.

  20. Numerical analysis of resonances induced by s wave neutrons in transmission time-of-flight experiments with a computer IBM 7094 II; Methodes d'analyse des resonances induites par les neutrons s dans les experiences de transmission par temps de vol et automatisation de ces methodes sur ordinateur IBM 7094 II

    Energy Technology Data Exchange (ETDEWEB)

    Corge, Ch [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

    1969-01-01

    Numerical analysis of transmission resonances induced by s wave neutrons in time-of-flight experiments can be achieved in a fairly automatic way on an IBM 7094/II computer. The involved computations are carried out following a four step scheme: 1 - experimental raw data are processed to obtain the resonant transmissions, 2 - values of experimental quantities for each resonance are derived from the above transmissions, 3 - resonance parameters are determined using a least square method to solve the over determined system obtained by equalling theoretical functions to the correspondent experimental values. Four analysis methods are gathered in the same code, 4 - graphical control of the results is performed. (author) [French] L'automatisation, sur ordinateur IBM 7094/II, de l'analyse des resonances induites par les neutrons s dans les experiences de transmission par temps de vol a ete accomplie en la decomposant selon un schema articule en quatre phases: 1 - le traitement des donnees experimentales brutes pour obtenir les transmissions interfero-resonnantes, 2 - la determination des grandeurs d'analyse a partir des transmissions precedentes, 3 - l'analyse proprement dite des resonances dont les parametres sont obtenus par la resolution d'un systeme surabondant. Quatre methodes d'analyse sont groupees en un meme programme, 4 - la procedure de verification graphique. (auteur)

  1. Numerical analysis of resonances induced by s wave neutrons in transmission time-of-flight experiments with a computer IBM 7094 II; Methodes d'analyse des resonances induites par les neutrons s dans les experiences de transmission par temps de vol et automatisation de ces methodes sur ordinateur IBM 7094 II

    Energy Technology Data Exchange (ETDEWEB)

    Corge, Ch. [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

    1969-01-01

    Numerical analysis of transmission resonances induced by s wave neutrons in time-of-flight experiments can be achieved in a fairly automatic way on an IBM 7094/II computer. The involved computations are carried out following a four step scheme: 1 - experimental raw data are processed to obtain the resonant transmissions, 2 - values of experimental quantities for each resonance are derived from the above transmissions, 3 - resonance parameters are determined using a least square method to solve the over determined system obtained by equalling theoretical functions to the correspondent experimental values. Four analysis methods are gathered in the same code, 4 - graphical control of the results is performed. (author) [French] L'automatisation, sur ordinateur IBM 7094/II, de l'analyse des resonances induites par les neutrons s dans les experiences de transmission par temps de vol a ete accomplie en la decomposant selon un schema articule en quatre phases: 1 - le traitement des donnees experimentales brutes pour obtenir les transmissions interfero-resonnantes, 2 - la determination des grandeurs d'analyse a partir des transmissions precedentes, 3 - l'analyse proprement dite des resonances dont les parametres sont obtenus par la resolution d'un systeme surabondant. Quatre methodes d'analyse sont groupees en un meme programme, 4 - la procedure de verification graphique. (auteur)

  2. Trajectory data analyses for pedestrian space-time activity study.

    Science.gov (United States)

    Qi, Feng; Du, Fei

    2013-02-25

    It is well recognized that human movement in the spatial and temporal dimensions has direct influence on disease transmission(1-3). An infectious disease typically spreads via contact between infected and susceptible individuals in their overlapped activity spaces. Therefore, daily mobility-activity information can be used as an indicator to measure exposures to risk factors of infection. However, a major difficulty and thus the reason for paucity of studies of infectious disease transmission at the micro scale arise from the lack of detailed individual mobility data. Previously in transportation and tourism research detailed space-time activity data often relied on the time-space diary technique, which requires subjects to actively record their activities in time and space. This is highly demanding for the participants and collaboration from the participants greatly affects the quality of data(4). Modern technologies such as GPS and mobile communications have made possible the automatic collection of trajectory data. The data collected, however, is not ideal for modeling human space-time activities, limited by the accuracies of existing devices. There is also no readily available tool for efficient processing of the data for human behavior study. We present here a suite of methods and an integrated ArcGIS desktop-based visual interface for the pre-processing and spatiotemporal analyses of trajectory data. We provide examples of how such processing may be used to model human space-time activities, especially with error-rich pedestrian trajectory data, that could be useful in public health studies such as infectious disease transmission modeling. The procedure presented includes pre-processing, trajectory segmentation, activity space characterization, density estimation and visualization, and a few other exploratory analysis methods. Pre-processing is the cleaning of noisy raw trajectory data. We introduce an interactive visual pre-processing interface as well as an

  3. Urokinase-type plasminogen activator receptor (uPAR) ligation induces a raft-localized integrin signaling switch that mediates the hypermotile phenotype of fibrotic fibroblasts.

    Science.gov (United States)

    Grove, Lisa M; Southern, Brian D; Jin, Tong H; White, Kimberly E; Paruchuri, Sailaja; Harel, Efrat; Wei, Ying; Rahaman, Shaik O; Gladson, Candece L; Ding, Qiang; Craik, Charles S; Chapman, Harold A; Olman, Mitchell A

    2014-05-02

    The urokinase-type plasminogen activator receptor (uPAR) is a glycosylphosphatidylinositol-linked membrane protein with no cytosolic domain that localizes to lipid raft microdomains. Our laboratory and others have documented that lung fibroblasts from patients with idiopathic pulmonary fibrosis (IPF) exhibit a hypermotile phenotype. This study was undertaken to elucidate the molecular mechanism whereby uPAR ligation with its cognate ligand, urokinase, induces a motile phenotype in human lung fibroblasts. We found that uPAR ligation with the urokinase receptor binding domain (amino-terminal fragment) leads to enhanced migration of fibroblasts on fibronectin in a protease-independent, lipid raft-dependent manner. Ligation of uPAR with the amino-terminal fragment recruited α5β1 integrin and the acylated form of the Src family kinase, Fyn, to lipid rafts. The biological consequences of this translocation were an increase in fibroblast motility and a switch of the integrin-initiated signal pathway for migration away from the lipid raft-independent focal adhesion kinase pathway and toward a lipid raft-dependent caveolin-Fyn-Shc pathway. Furthermore, an integrin homologous peptide as well as an antibody that competes with β1 for uPAR binding have the ability to block this effect. In addition, its relative insensitivity to cholesterol depletion suggests that the interactions of α5β1 integrin and uPAR drive the translocation of α5β1 integrin-acylated Fyn signaling complexes into lipid rafts upon uPAR ligation through protein-protein interactions. This signal switch is a novel pathway leading to the hypermotile phenotype of IPF patient-derived fibroblasts, seen with uPAR ligation. This uPAR dependent, fibrotic matrix-selective, and profibrotic fibroblast phenotype may be amenable to targeted therapeutics designed to ameliorate IPF.

  4. Thermal, intermediate and fast neutron flux measurements using activation detectors; Mesure des flux de neutrons thermiques, intermediaires et rapides au moyen de detecteurs par activation

    Energy Technology Data Exchange (ETDEWEB)

    Brisbois, J; Lott, M; Manent, G [Commissariat a l' Energie Atomique, Fontenay-aux-Roses (France). Centre d' Etudes Nucleaires

    1964-07-01

    The problem of neutron flux measurements using activation detectors is studied in the particular case of protection research. It is shown how it possible, it is possible, using a known thermal flux, to organise a coherent calibration system for all the detectors. The rapid neutron detectors are calibrated with respect to a reference detector (phosphorus) in a natural uranium converter; the intermediate neutron detectors with respect to gold in the axial channel of ZOE. This method makes it possible to minimise the errors due to the activation cross-sections. A brief description is given of the counting room of the Pile Safety Study Service, as well of the practical utilisation characteristics of the counters employed. (authors) [French] Le probleme de la mesure des flux de neutrons au moyen de detecteurs par activation est etudie dans le cas particulier des etudes de protections. On montre comment, a partir d'un flux thermique connu, on peut organiser un systeme coherent d'etalonnage de tous les detecteurs. Les detecteurs de neutrons rapides sont etalonnes par rapport a un detecteur de reference (phosphore) dans un convertisseur en uranium naturel; les detecteurs de neutrons intermediaires, par rapport a l'or dans le canal axial de ZOE, Cette methode permet de minimiser les erreurs dues aux sections efficaces d'activation. On decrit sommairement la salle de comptage du Service d'Etudes de Protections de Piles et on indique les caracteristiques d'emploi pratique des detecteurs utilises. (auteurs)

  5. Activation and Shielding Analyses in Support of the GUINEVERE Project

    Energy Technology Data Exchange (ETDEWEB)

    Serikov, A.; Fischer, U.; Mercatali, L. [Association FZK-EURATOM, KIT, Forschungszentrum Karlsruhe, P.O. Box 3640, 76021 Karlsruhe (Germany); Baeten, P.; Vittiglio, G. [SCK-CEN, Boeretang 200, B-2400 Mol (Belgium)

    2008-07-01

    The GUINEVERE facility (Generator of Uninterrupted Intense Neutrons at the lead Venus Reactor) must satisfy the nuclear safety criteria required by the Belgian safety authority to be licensed. The radiation dose and activation analyses for the nuclear safety assessment of the GUINEVERE project were performed at FZK. The concerted efforts of several European institutions were concentrated on the development and construction of a subcritical fast lead core based on the Venus water moderated reactor at the SCK-CEN site in Mol, Belgium. A Monte Carlo (MC) MCNP5 model was developed in accordance with the current design of the GUINEVERE fast lead core. The analytical MC method does not work for shielding analysis of the GUINEVERE building because of the large size of the rooms and thick concrete walls and floors. MC variance reduction techniques, such as particles splitting, Russian roulette, and point detectors were therefore applied. The JEFF-3.1 nuclear data library was used for radiation transport calculations. The activation analyses for the lead core and building materials were performed with the FISPACT-2005 inventory code and the EAF-2005 library. The neutron and photon dose rate maps were produced using MCNP track-length estimations, point detectors, and a mesh tally superimposed over the GUINEVERE geometry. The effects of D-D and D-T fusion neutron sources were estimated. (authors)

  6. Activation and Shielding Analyses in Support of the GUINEVERE Project

    International Nuclear Information System (INIS)

    Serikov, A.; Fischer, U.; Mercatali, L.; Baeten, P.; Vittiglio, G.

    2008-01-01

    The GUINEVERE facility (Generator of Uninterrupted Intense Neutrons at the lead Venus Reactor) must satisfy the nuclear safety criteria required by the Belgian safety authority to be licensed. The radiation dose and activation analyses for the nuclear safety assessment of the GUINEVERE project were performed at FZK. The concerted efforts of several European institutions were concentrated on the development and construction of a subcritical fast lead core based on the Venus water moderated reactor at the SCK-CEN site in Mol, Belgium. A Monte Carlo (MC) MCNP5 model was developed in accordance with the current design of the GUINEVERE fast lead core. The analytical MC method does not work for shielding analysis of the GUINEVERE building because of the large size of the rooms and thick concrete walls and floors. MC variance reduction techniques, such as particles splitting, Russian roulette, and point detectors were therefore applied. The JEFF-3.1 nuclear data library was used for radiation transport calculations. The activation analyses for the lead core and building materials were performed with the FISPACT-2005 inventory code and the EAF-2005 library. The neutron and photon dose rate maps were produced using MCNP track-length estimations, point detectors, and a mesh tally superimposed over the GUINEVERE geometry. The effects of D-D and D-T fusion neutron sources were estimated. (authors)

  7. Structure, function and expression on blood and bone marrow cells of the urokinase-type plasminogen activator receptor, uPAR

    DEFF Research Database (Denmark)

    Plesner, T; Behrendt, N; Ploug, M

    1997-01-01

    patients with the rare blood disease paroxysmal nocturnal hemoglobinuria (PNH) that fail to express glycosyl-phosphatidylinositol-anchored proteins including uPAR, show a very significantly reduced transmigration over an endothelial barrier. Cell-associated plasminogen activation by PNH......Several important functions have been assigned to the receptor for urokinase-type plasminogen activator, uPAR. As implied by the name, uPAR was first identified as a high affinity cellular receptor for urokinase plasminogen activator (uPA). It mediates the binding of the zymogen, pro......-uPA, to the plasma membrane where trace amounts of plasmin will initiate a series of events referred to as "reciprocal zymogen activation" where plasmin converts pro-uPA to the active enzyme, uPA, which in turn converts plasma membrane-associated plasminogen to plasmin. This is an efficient machinery to generate...

  8. Activation and afterheat analyses for the HCPB test blanket

    International Nuclear Information System (INIS)

    Pereslavtsev, P.; Fischer, U.

    2007-01-01

    The Helium-Cooled Pebble Bed (HCPB) blanket is one of two breeder blanket concepts developed in the framework of the European Fusion Technology Programme for performance tests in ITER. The recent development programme focussed on the detailed engineering design of the Test Blanket Module (TBM) and associated systems including the assessment of safety and licensing related issues with the objective to prepare for a preliminary Safety Report. To provide a sound data basis for the safety analyses of the HCPB TBM system in ITER, the afterheat and activity inventories were assessed making use of a code system that allows performing 3D activation calculations by linking the Monte Carlo transport code MCNP and the fusion inventory code FISPACT through an appropriate interface. A suitable MCNP model of a 20 degree ITER torus sector with an integrated TBM of the HCPB PI (Plant Integration) type in the horizontal test blanket port was developed and adapted to the requirements for coupled 3D neutron transport and activation calculations. Two different irradiation scenarios were considered in the coupled 3D neutron transport and activation calculations. The first one is representative for the TBM irradiation in ITER with a total of 9000 neutron pulses over a three (calendar) years period. It was simulated by a continuous irradiation for 3 years minus the last month and a discontinuous irradiation with 250 pulses (420 s pulse length, 1200 s power-off in between) over the last month. The second (conservative) irradiation scenario assumes an extended irradiation time over the full anticipated lifetime of ITER according to the M-DRG-1 irradiation scenario with a total first wall fluence of 0.3 MWa/m 2 . For both irradiation scenarios the radioactivity inventories, the afterheat and the contact gamma dose were calculated as function of the decay time. Data were processed for the total activity and afterheat of the TBM, its constituting components and materials including their

  9. PAR-2 activation enhances weak acid-induced ATP release through TRPV1 and ASIC sensitization in human esophageal epithelial cells.

    Science.gov (United States)

    Wu, Liping; Oshima, Tadayuki; Shan, Jing; Sei, Hiroo; Tomita, Toshihiko; Ohda, Yoshio; Fukui, Hirokazu; Watari, Jiro; Miwa, Hiroto

    2015-10-15

    Esophageal visceral hypersensitivity has been proposed to be the pathogenesis of heartburn sensation in nonerosive reflux disease. Protease-activated receptor-2 (PAR-2) is expressed in human esophageal epithelial cells and is believed to play a role in inflammation and sensation. PAR-2 activation may modulate these responses through adenosine triphosphate (ATP) release, which is involved in transduction of sensation and pain. The transient receptor potential vanilloid receptor 1 (TRPV1) and acid-sensing ion channels (ASICs) are both acid-sensitive nociceptors. However, the interaction among these molecules and the mechanisms of heartburn sensation are still not clear. We therefore examined whether ATP release in human esophageal epithelial cells in response to acid is modulated by TRPV1 and ASICs and whether PAR-2 activation influences the sensitivity of TRPV1 and ASICs. Weak acid (pH 5) stimulated the release of ATP from primary human esophageal epithelial cells (HEECs). This effect was significantly reduced after pretreatment with 5-iodoresiniferatoxin (IRTX), a TRPV1-specific antagonist, or with amiloride, a nonselective ASIC blocker. TRPV1 and ASIC3 small interfering RNA (siRNA) transfection also decreased weak acid-induced ATP release. Pretreatment of HEECs with trypsin, tryptase, or a PAR-2 agonist enhanced weak acid-induced ATP release. Trypsin treatment led to the phosphorylation of TRPV1. Acid-induced ATP release enhancement by trypsin was partially blocked by IRTX, amiloride, or a PAR-2 antagonist. Conversely, acid-induced ATP release was augmented by PAR-2 activation through TRPV1 and ASICs. These findings suggested that the pathophysiology of heartburn sensation or esophageal hypersensitivity may be associated with the activation of PAR-2, TRPV1, and ASICs. Copyright © 2015 the American Physiological Society.

  10. A Brief overview of neutron activation analyses methodology and applications

    International Nuclear Information System (INIS)

    Ali, M.A.

    2000-01-01

    The primary objective of this talk is to present our new facility for Neutron Activation Analysis to the scientific and industrial societies and show its possibilities. Therefore my talk will handle the following main items: An overview of neutron activation analysis, The special interest of fast mono-energetic neutrons, The NAA method and its sensitivities, The Recent scientific and industrial applications using NAA, and o An illustrating example measured by using our facility is presented What is NAA? It is a sensitive analytical technique useful for performing both qualitative and quantitative multi-element analyses in samples. Worldwide application of NAA is so widespread; it is estimated that approximately several 10,000 samples undergo analysis each year from almost every conceivable field of scientific or technical interest. Why NAA? For many elements and applications, NAA: Offers sensitivities that are sometimes superior to those attainable by other methods, on the order of nano-gram level, It is accurate and reliable, NAA is generally recognized as the r eferee method o f choice when new procedures are being developed or when other methods yield results that do not agree. However, the activation analysis at En=14 MeV is limited by a few factors: Low value of flux, low cross-sections of threshold reactions, o Short irradiation time due to finite target life, Interfering reactions and gamma ray spectral interference

  11. Contribution to the analysis of light elements using x fluorescence excited by radio-elements; Contribution a l'analyse des elements legers par fluorescence x excitee au moyen de radioelements

    Energy Technology Data Exchange (ETDEWEB)

    Robert, A [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

    1964-07-01

    In order to study the possibilities of using radioactive sources for the X-fluorescence analysis of light elements, the principle is given, after a brief description of X-fluorescence, of the excitation of this phenomenon by X, {beta} and {alpha} emission from radio-elements. The operation and use of the proportional gas counter for X-ray detection is described. A device has been studied for analysing the elements of the 2. and 3. periods of the Mendeleev table. It makes it possible to excite the fluorescence with a radioactive source emitting X-rays or a particles; the X-ray fluorescence penetrates into a window-less proportional counter, this being made possible by the use of an auxiliary electric field in the neighbourhood of the sample. The gas detection pressure leading to the maximum detection yield is given. The spectra are given for the K{sub {alpha}} lines of 3. period elements excited by {sup 55}Fe, {sup 3}H/Zr and {sup 210}Po sources; for the 2. period the K{sub {alpha}} spectra of carbon and of fluorine excited by the {alpha} particles of {sup 210}Po. (author) [French] Afin d'etudier les possibilites d'emploi de sources radioactives a l'analyse par fluorescence X des elements legers, on presente apres rappel de notions generales sur la fluorescence X, le principe de l'excitation de ce phenomene par emission X, {beta}, {alpha} de radioelements. Le fonctionnement et l'utilisation du compteur proportionnel a gaz a la detection du rayonnement X est developpe. Un dispositif permettant l'analyse des elements des 2eme et 3eme periodes de la classification de Mendeleev est etudie. Il permet l'excitation de la fluorescence par source radioactive emettrice de rayons X ou de particules {alpha}; le rayonnement X de fluorescence penetre dans un compteur proportionnel depourvu de fenetre, ceci est rendu possible en creant un champ electrique auxiliaire au voisinage de l'echantillon. On definit une pression du gaz de detection pour un rendement de detection maximal

  12. Contribution to the analysis of light elements using x fluorescence excited by radio-elements; Contribution a l'analyse des elements legers par fluorescence x excitee au moyen de radioelements

    Energy Technology Data Exchange (ETDEWEB)

    Robert, A. [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

    1964-07-01

    In order to study the possibilities of using radioactive sources for the X-fluorescence analysis of light elements, the principle is given, after a brief description of X-fluorescence, of the excitation of this phenomenon by X, {beta} and {alpha} emission from radio-elements. The operation and use of the proportional gas counter for X-ray detection is described. A device has been studied for analysing the elements of the 2. and 3. periods of the Mendeleev table. It makes it possible to excite the fluorescence with a radioactive source emitting X-rays or a particles; the X-ray fluorescence penetrates into a window-less proportional counter, this being made possible by the use of an auxiliary electric field in the neighbourhood of the sample. The gas detection pressure leading to the maximum detection yield is given. The spectra are given for the K{sub {alpha}} lines of 3. period elements excited by {sup 55}Fe, {sup 3}H/Zr and {sup 210}Po sources; for the 2. period the K{sub {alpha}} spectra of carbon and of fluorine excited by the {alpha} particles of {sup 210}Po. (author) [French] Afin d'etudier les possibilites d'emploi de sources radioactives a l'analyse par fluorescence X des elements legers, on presente apres rappel de notions generales sur la fluorescence X, le principe de l'excitation de ce phenomene par emission X, {beta}, {alpha} de radioelements. Le fonctionnement et l'utilisation du compteur proportionnel a gaz a la detection du rayonnement X est developpe. Un dispositif permettant l'analyse des elements des 2eme et 3eme periodes de la classification de Mendeleev est etudie. Il permet l'excitation de la fluorescence par source radioactive emettrice de rayons X ou de particules {alpha}; le rayonnement X de fluorescence penetre dans un compteur proportionnel depourvu de fenetre, ceci est rendu possible en creant un champ electrique auxiliaire au voisinage de l'echantillon. On definit une pression du gaz de detection

  13. Leçons d’analyse classique exposition d'un cours fait par Paul Koosis à l'Université McGill, Montréal

    CERN Document Server

    Poulin, Philippe

    2015-01-01

    Ce livre est basé sur un cours de deuxième cycle donné en 2005-2006 par M. Paul Koosis, professeur émérite à l'université McGill. Il traite de sujets soigneusement choisis par le professeur à l'intention de ceux qui, plutôt que de rechercher un catalogue exhaustif de résultats techniques et abstraits, veulent être initiés aux découvertes les plus essentielles et prolifiques de l'analyse classique du vingtième siècle. Analyse harmonique, quasi-analyticité, zéros des fonctions entières (dont une preuve inédite du théorème de Levinson-Cartwright), approximation pondérée, principe d'incertitude, mesures harmoniques…, les résultats saillants et géniaux de l'analyse classique sont présentés dans un style soigné, rigoureux et détaillé, préparant les étudiants à des études plus poussées ; et au service du lecteur qui, connaissant les bases de la théorie de la mesure et de l'analyse complexe, désire suivre le merveilleux développement de M. Koosis et accroître sa connaissance d...

  14. Practical Applications of Short-Lived Radionuclides in Activation Analysis; Applications Pratiques des Radioelements de Courte Periode dans l'Analyse par Activation; ПРАКТИЧЕСКОЕ ПРИМЕНЕНИЕ КОРОТКОЖИВУЩИХ РАДИОИЗОТОПОВ ПРИ АКТИВАЦИОННОМ АНАЛИЗЕ; Aplicaciones Practicas de los Radionuclidos de Periodo Corto en el Anausis por Activacion

    Energy Technology Data Exchange (ETDEWEB)

    Chinaglia, B. Chin; Ciuffolotti, L.; Fasolo, G. B.; Malvano, R. [Centre de Recherches Nucleaires Sorin, Saluggia (Italy)

    1963-03-15

    Activation analysis by means of short-lived radionuclides has found some remarkable applications in the SORIN Research Centre at Saluggia. The paper describes the special techniques of irradiation, chemical separation and measurement required to obtain adequate accuracy and good sensitivity. It has proved possible to apply the method to analysis of elements with a half-life down to 10 s, with a consequent enormous gain in time. The method has been of benefit in several different spheres, as in solving metallurgical and technical problems (e.g. study of impurities in special-purpose materials) and in geochemistry and biology. (author) [French] L'analyse par activation au moyen de radioelements de courte periode a trouve de remarquables applications au Centre de recherches SORIN, a Saluggia. On decrit dans le memoire les techniques particulieres d'irradiation, de separation chimique et de mesure qui sont indispensables pour obtenir une precision satisfaisante ainsi qu'une bonne sensibilite. On a pu ainsi etendre la methode a l'analyse dfelements dont la periode peut atteindre dix secondes, realisant de cette facon un remarquable gain de temps. Plusieurs branches ont tire profit de cette methode, soit dans la resolution de problemes metallurgiques et technologiques (par exemple etude des impuretes dans des materiaux d'emploi special), soit en geochimie et en biologie. (author) [Spanish] El analisis por activacion empleando radionuclidos de periodo corto ha encontrado aplicaciones notables en el Centro de Investigaciones SORIN de Saluggia. En la memoria se describen tecnicas especiales de irradiacion, de separacion quimica y de medicion, indispensables para alcanzar una precision y sensibilidad satisfactorias. Se han podido analizar elementos de un periodo de hasta 10 s, logrando asi un considerable ahorro de tiempo. El metodo ha beneficiado a muchos campos de aplicacion, sea para resolver problemas de metalurgia y technologia (por ejemplo en el estudio de las

  15. Elevated urinary levels of urokinase-type plasminogen activator receptor (uPAR) in pancreatic ductal adenocarcinoma identify a clinically high-risk group

    International Nuclear Information System (INIS)

    Sorio, Claudio; Scarpa, Aldo; Mafficini, Andrea; Furlan, Federico; Barbi, Stefano; Bonora, Antonio; Brocco, Giorgio; Blasi, Francesco; Talamini, Giorgio; Bassi, Claudio

    2011-01-01

    The urokinase plasminogen activator receptor is highly expressed and its gene is amplified in about 50% of pancreatic ductal adenocarcinomas; this last feature is associated with worse prognosis. It is unknown whether the level of its soluble form (suPAR) in urine may be a diagnostic-prognostic marker in these patients. The urinary level of suPAR was measured in 146 patients, 94 pancreatic ductal adenocarcinoma and 52 chronic pancreatitis. Urine from 104 healthy subjects with similar age and gender distribution served as controls. suPAR levels were normalized with creatinine levels (suPAR/creatinine, ng/mg) to remove urine dilution effect. Urinary suPAR/creatinine values of pancreatic ductal adenocarcinoma patients were significantly higher (median 9.8; 25 th -75 th percentiles 5.3-20.7) than those of either healthy donors (median 0; 0-0.5) or chronic pancreatitis patients (median 2.7; 0.9-4.7). The distribution of values among cancer patients was widespread and asymmetric, 53% subjects having values beyond the 95 th percentile of healthy donors. The values of suPAR/creatinine did not correlate with tumour stage, Ca19-9 or CEA levels. Higher values correlated with poor prognosis among non-resected patients at univariate analysis; multivariate Cox regression identified high urinary suPAR/creatinine as an independent predictor of poor survival among all cancer patients (odds ratio 2.10, p = 0.0023), together with tumour stage (stage III odds ratio 2.65, p = 0.0017; stage IV odds ratio 4.61, p < 0.0001) and female gender (odds ratio 1.85, p = 0.01). A high urinary suPAR/creatinine ratio represents a useful marker for the identification of a subset of patients with poorer outcome

  16. Plasma levels of soluble urokinase-type plasminogen activator receptor (suPAR and early mortality risk among patients enrolling for antiretroviral treatment in South Africa

    Directory of Open Access Journals (Sweden)

    Bangani Nonzwakazi

    2007-05-01

    Full Text Available Abstract Background Serum concentrations of soluble urokinase-type plasminogen activator receptor (suPAR have a strong independent association with HIV-1-related mortality. The practical utility of plasma suPAR in assessing short-term all-cause mortality risk was evaluated in patients with advanced immunodeficiency enrolling in an antiretroviral treatment (ART programme in South Africa. Methods An enzyme-linked immunosorbent assay (ELISA was used to measure plasma concentrations of suPAR in patients at the time of enrolment to the ART programme. The association between plasma suPAR concentrations, baseline patient characteristics and cohort outcomes after 4 months of ART were determined. Results Patients (n = 293, 70% female had a median age of 33 years and were followed up for a median of 5 months from enrolment. The median CD4 cell count was 47 cells/μl (IQR = 22–72 and 38% of patients had WHO stage 4 disease. 218 (74% patients remained alive after 4 months of ART; 39 (13% died and 36 (12% were lost to the programme for other reasons. Patients who died had significantly higher plasma suPAR concentrations compared to those who either survived (P 10 suPAR concentrations were significantly associated with lower CD4 cell counts, WHO clinical stage 4 disease and male sex. In multivariate analysis to identify factors associated with death, log10 suPAR concentration was the most strongly associated variable (P Conclusion Plasma suPAR concentration was the strongest independent predictor of short-term mortality risk among patients with advanced immunodeficiency enrolling in this ART programme. However, lack of a discriminatory threshold did not permit this marker to be used to triage patients according to short-term mortality risk.

  17. Diagnostic accuracy of soluble urokinase plasminogen activator receptor (suPAR) for prediction of bacteremia in patients with systemic inflammatory response syndrome.

    Science.gov (United States)

    Hoenigl, Martin; Raggam, Reinhard B; Wagner, Jasmin; Valentin, Thomas; Leitner, Eva; Seeber, Katharina; Zollner-Schwetz, Ines; Krammer, Werner; Prüller, Florian; Grisold, Andrea J; Krause, Robert

    2013-02-01

    Soluble urokinase plasminogen activator receptor (suPAR) serum concentrations have recently been described to reflect the severity status of systemic inflammation. In this study, the diagnostic accuracy of suPAR, C-reactive protein (CRP), procalcitonin (PCT), and interleukin-6 (IL-6) to predict bacteremia in patients with systemic inflammatory response syndrome (SIRS) was compared. A total of 132 patients with SIRS were included. In 55 patients blood cultures had resulted positive (study group 1, Gram positive bacteria: Staphylococcus aureus and Streptococcus spp., n=15; study group 2, Gram-negative bacteria, n=40) and 77 patients had negative blood culture results (control group, n=77). Simultaneously with blood cultures suPAR, CRP, PCT, IL-6 and white blood count (WBC) were determined. SuPAR values were significantly higher in study group 1 (median 8.11; IQR 5.78-15.53; p=0.006) and study group 2 (median 9.62; IQR 6.52-11.74; p<0.001) when compared with the control group (median 5.65; IQR 4.30-7.83). ROC curve analysis revealed an AUC of 0.726 for suPAR in differentiating SIRS patients with bacteremia from those without. The biomarkers PCT and IL-6 showed comparable results. Regarding combinations of biomarkers multiplying suPAR, PCT and IL-6 was most promising and resulted in an AUC value of 0.804. Initial suPAR serum concentrations were significantly higher (p=0.028) in patients who died within 28 days than in those who survived. No significant difference was seen for PCT, IL-6 and CRP. In conclusion, suPAR, IL-6 and PCT may contribute to predicting bacteremia in SIRS patients. Copyright © 2012 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.

  18. The urban brain: analysing outdoor physical activity with mobile EEG.

    Science.gov (United States)

    Aspinall, Peter; Mavros, Panagiotis; Coyne, Richard; Roe, Jenny

    2015-02-01

    Researchers in environmental psychology, health studies and urban design are interested in the relationship between the environment, behaviour settings and emotions. In particular, happiness, or the presence of positive emotional mindsets, broadens an individual's thought-action repertoire with positive benefits to physical and intellectual activities, and to social and psychological resources. This occurs through play, exploration or similar activities. In addition, a body of restorative literature focuses on the potential benefits to emotional recovery from stress offered by green space and 'soft fascination'. However, access to the cortical correlates of emotional states of a person actively engaged within an environment has not been possible until recently. This study investigates the use of mobile electroencephalography (EEG) as a method to record and analyse the emotional experience of a group of walkers in three types of urban environment including a green space setting. Using Emotiv EPOC, a low-cost mobile EEG recorder, participants took part in a 25 min walk through three different areas of Edinburgh. The areas (of approximately equal length) were labelled zone 1 (urban shopping street), zone 2 (path through green space) and zone 3 (street in a busy commercial district). The equipment provided continuous recordings from five channels, labelled excitement (short-term), frustration, engagement, long-term excitement (or arousal) and meditation. A new form of high-dimensional correlated component logistic regression analysis showed evidence of lower frustration, engagement and arousal, and higher meditation when moving into the green space zone; and higher engagement when moving out of it. Systematic differences in EEG recordings were found between three urban areas in line with restoration theory. This has implications for promoting urban green space as a mood-enhancing environment for walking or for other forms of physical or reflective activity. Published

  19. Measurement of a thermal neutron flux using air activation; Mesure de flux de neutrons thermiques par activation d'air

    Energy Technology Data Exchange (ETDEWEB)

    Guyonvarh, M; Lecomte, P; Le Meur, R [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

    1967-07-01

    It is necessary to know, in irradiation loops, the thermal neutron flux after the irradiation device has been introduced and without being obliged to wait for the discharge of this device. In order to measure the flux and to control it continuously, one possible method is to place in the flux a coiled steel tube through which air passes. By measuring the activity of argon 41, and with a knowledge of the flow rate and the temperature of the air, it is possible to calculate the flux. An air-circulation flux controller is described and the relationship between the flux and the count rate is established The accuracy of an absolute measurement is about 14 per cent; that of a relative measurement is about 3 per cent. The measurement can be carried out equally well whether the reactor is operating at maximum or at low power. The measurement range goes from 10{sup 9} to lO{sup 15} n.cm{sup -2}.sec{sup -1}, and it would be possible after a few modifications to measure fluxes between 10{sup 5} and 10{sup 15} n.cm{sup -2}.sec{sup -1}. Finally, the method is very safe to operate: there is little risk of irradiation because of the low specific activity of the argon-41 formed, and no risk of contamination because the decay product of argon-41 is stable. This method, which is now being used in loops, is thus very practical. (authors) [French] Sur des boucles d'irradiation il est necessaire de connaitre le flux de neutrons thermiques apres mise en place du dispositif d'irradiation et sans etre oblige d'attendre le detournement de ce dispositif. Pour mesurer le flux et le controler en permanence, une methode consiste a placer sous flux un serpentin en acier dans lequel on fait circuler de l'air. La mesure d'activite d'argon 41 permet de calculer le flux, connaissant le debit et la temperature de l'air. Un controleur de flux par circulation d'air est decrit et la relation entre le flux et le taux de comptage est etablie. La precision d'une mesure absolue est de l'ordre de 14 pour

  20. Activation analyses updating the ITER radioactive waste assessment

    International Nuclear Information System (INIS)

    Pampin, R.; Zheng, S.; Lilley, S.; Na, B.C.; Loughlin, M.J.; Taylor, N.P.

    2012-01-01

    Highlights: ► Comprehensive updated of ITER radwaste assessment. ► Latest coupled neutronics and activation methods. ► Type A waste at shutdown decays to TFA within 100 years. ► Most type B waste at shutdown is still type B after 100 years. - Abstract: A study is reported which computes the radiation transport and activation response throughout the ITER machine and updates the ITER radioactive waste assessment using modern 3D models and up-to-date methods. The latest information on component design, maintenance, replacement schedules and materials is adopted. The radwaste classification is revised for all the major components of ITER, as well as several representative port plugs. Results include categorisation snapshots at different decay times, time histories of radiological quantities throughout the machine, and guidelines on interim decay times for components. All plasma-facing materials except tungsten are found to classify as type B due to the transmutation of their main constituents. Major contributors to the IRAS index of all materials are reported. Elemental concentration limits for type A classification of first wall and divertor materials are obtained; for the steels, only a reduction in service lifetime can reduce the waste class. Comparison of total waste amounts with earlier assessments is limited by the fact that analyses of some components are still preliminary; the trend, however, indicates a potential reduction in the total amount of waste if component segregation is demonstrated.

  1. Psychometric analyses to improve the Dutch ICF Activity Inventory.

    Science.gov (United States)

    Bruijning, Janna E; van Rens, Ger; Knol, Dirk; van Nispen, Ruth

    2013-08-01

    In the past, rehabilitation centers for the visually impaired used unstructured or semistructured methods to assess rehabilitation needs of their patients. Recently, an extensive instrument, the Dutch ICF Activity Inventory (D-AI), was developed to systematically investigate rehabilitation needs of visually impaired adults and to evaluate rehabilitation outcomes. The purpose of this study was to investigate the underlying factor structure and other psychometric properties to shorten and improve the D-AI. The D-AI was administered to 241 visually impaired persons who recently enrolled in a multidisciplinary rehabilitation center. The D-AI uses graded scores to assess the importance and difficulty of 65 rehabilitation goals. For high-priority goals (e.g., daily meal preparation), the difficulty of underlying tasks (e.g., read recipes, cut vegetables) was assessed. To reduce underlying task items (>950), descriptive statistics were investigated and factor analyses were performed for several goals. The internal consistency reliability and test-retest reliability of the D-AI were investigated by calculating Cronbach α and Cohen (weighted) κ. Finally, consensus-based discussions were used to shorten and improve the D-AI. Except for one goal, factor analysis model parameters were at least reasonable. Internal consistency reliability was satisfactory (range, 0.74 to 0.93). In total, 60% of the 65 goal importance items and 84.4% of the goal difficulty items showed moderate to almost perfect κ values (≥0.40). After consensus-based discussions, a new D-AI was produced, containing 48 goals and less than 500 tasks. The analyses were an important step in the validation process of the D-AI and to develop a more feasible assessment tool to investigate rehabilitation needs of visually impaired persons in a systematic way. The D-AI is currently implemented in all Dutch rehabilitation centers serving all visually impaired adults with various rehabilitation needs.

  2. Guide d'exploitation des filières de traitement par boues activées

    OpenAIRE

    Boutin, C.; Caquel, O.; Dimastromatteo, N.,; Dumaine, J.,; Fernandes. G.,; Gervasi, C.; Parotin, S.; Prost Boucle, S.; Tscherter, C.

    2015-01-01

    / Ce guide, à destination des maîtres d'ouvrage et des exploitants, a pour objet de présenter de façon synthétique, les modalités d'exploitation courante d'une station d'épuration par boues activées de capacité < 600 kg DBO5/jour (soit 10 000 équivalents habitants (EH)), et dimensionnée dans le domaine de charge de l'aération prolongée (Cm < 0,1 kg DBO5 /kg MVS/jour). Conçu au fil de l'eau, il recense, tant pour la file eau que pour la file boues, l'ensemble des tâches d'exploitation courante...

  3. Neutrophilia, gelatinase release and microvascular leakage induced by human mast cell tryptase in a mouse model: Lack of a role of protease-activated receptor 2 (PAR2).

    Science.gov (United States)

    Khedr, M E M S; Abdelmotelb, A M; Pender, S L F; Zhou, X; Walls, A F

    2018-05-01

    Tryptase, the most abundant protease of the human mast cell, has been implicated as a key mediator of allergic inflammation that acts through activation of PAR2. To investigate the contribution of PAR2 in the pro-inflammatory actions mediated by tryptase in a mice model. We have injected recombinant human βII-tryptase into the peritoneum of PAR2-deficient and wild-type C57BL/6 mice. After 6, 12 and 24 hours, mice were killed, peritoneal lavage performed and inflammatory changes investigated. Tryptase stimulated an increase in neutrophil numbers in the peritoneum, but responses did not differ between PAR2-deficient and wild-type mice. Heat inactivation of tryptase or pre-incubation with a selective tryptase inhibitor reduced neutrophilia, but neutrophil accumulation was not elicited with a peptide agonist of PAR2 (SLIGRL-NH 2 ). Zymography indicated that tryptase stimulated the release of matrix metalloproteinases (MMP) 2 and 9 in the peritoneum of both mouse strains. Studies involving immunomagnetic isolation of neutrophils suggested that neutrophils represent the major cellular source of tryptase-induced MMP2 and MMP9. At 24 hours after tryptase injection, there was increased microvascular leakage as indicated by high levels of albumin in peritoneal lavage fluid, and this appeared to be partially abolished by heat-inactivating tryptase or addition of a protease inhibitor. There was no corresponding increase in levels of histamine or total protein. The extent of tryptase-induced microvascular leakage or gelatinase release into the peritoneum did not differ between PAR2-deficient and wild-type mice. Our findings indicate that tryptase is a potent stimulus for neutrophil accumulation, MMP release and microvascular leakage. Although these actions required an intact catalytic site, the primary mechanism of tryptase in vivo would appear to involve processes independent of PAR2. © 2018 The Authors. Clinical & Experimental Allergy Published by John Wiley & Sons Ltd.

  4. Regulator of G protein signaling 2 (RGS2 and RGS4 form distinct G protein-dependent complexes with protease activated-receptor 1 (PAR1 in live cells.

    Directory of Open Access Journals (Sweden)

    Sungho Ghil

    Full Text Available Protease-activated receptor 1 (PAR1 is a G-protein coupled receptor (GPCR that is activated by natural proteases to regulate many physiological actions. We previously reported that PAR1 couples to Gi, Gq and G12 to activate linked signaling pathways. Regulators of G protein signaling (RGS proteins serve as GTPase activating proteins to inhibit GPCR/G protein signaling. Some RGS proteins interact directly with certain GPCRs to modulate their signals, though cellular mechanisms dictating selective RGS/GPCR coupling are poorly understood. Here, using bioluminescence resonance energy transfer (BRET, we tested whether RGS2 and RGS4 bind to PAR1 in live COS-7 cells to regulate PAR1/Gα-mediated signaling. We report that PAR1 selectively interacts with either RGS2 or RGS4 in a G protein-dependent manner. Very little BRET activity is observed between PAR1-Venus (PAR1-Ven and either RGS2-Luciferase (RGS2-Luc or RGS4-Luc in the absence of Gα. However, in the presence of specific Gα subunits, BRET activity was markedly enhanced between PAR1-RGS2 by Gαq/11, and PAR1-RGS4 by Gαo, but not by other Gα subunits. Gαq/11-YFP/RGS2-Luc BRET activity is promoted by PAR1 and is markedly enhanced by agonist (TFLLR stimulation. However, PAR1-Ven/RGS-Luc BRET activity was blocked by a PAR1 mutant (R205A that eliminates PAR1-Gq/11 coupling. The purified intracellular third loop of PAR1 binds directly to purified His-RGS2 or His-RGS4. In cells, RGS2 and RGS4 inhibited PAR1/Gα-mediated calcium and MAPK/ERK signaling, respectively, but not RhoA signaling. Our findings indicate that RGS2 and RGS4 interact directly with PAR1 in Gα-dependent manner to modulate PAR1/Gα-mediated signaling, and highlight a cellular mechanism for selective GPCR/G protein/RGS coupling.

  5. Microhabitats utilisés par les alevins 0+ d'une communauté de cyprins rhéophiles : analyse de l'évolution de la communauté et des densités de population

    Directory of Open Access Journals (Sweden)

    BARAS E.

    1995-04-01

    Full Text Available L'évolution de l'occupation des microhabitats par les alevins de la communauté de cyprins rhéophiles de l'Ourthe a été étudiée au cours de l'été et de l'automne 1994 par échantillonnage ponctuel d'abondance (électrodes fixes prépositionnées, courant continu. 16155 poissons (10-75 mm appartenant à 15 espèces ont été recensés sur 78 sites d'échantillonnage de 2 m2 . Les espèces les plus représentées étaient Leuciscus cephalus, Leuciscus leuciscus et Chondrostoma nasus (respectivement 43,6 %, 20,7 % et 16,7 % des captures estivales. L'analyse factorielle des correspondances d'une matrice site x (espèce x taille met en évidence une subdivision progressive de la communauté au cours de l'été en espèces d'eau vive (B. barbus et L. leuciscus et d'eau lente, davantage associées à un couvert végétal (L. cephalus et C. nasus. La densité maximale observée (fin juin est de 1500 alevins (25 mm m-2, correspondant à une biomasse de 250 g m-2. Le modèle explicatif de la densité généré par analyse de régression multiple pas-à-pas explique 60,0 % de la variabilité de la densité par les variables vitesse de courant, température de l'eau et date d'échantillonnage, cette variable intégrant le concept de mortalité-dispersion au cours de la saison de croissance. Les implications de la méthodologie et des résultats présentés au plan de la gestion des écosystèmes lotiques sont brièvement discutés.

  6. Analyse des stratégies d’apprentissage dans une méthode d’apprentissage par problèmes : le cas d’étudiantes en soins infirmiers

    Directory of Open Access Journals (Sweden)

    Caroline Larue

    2009-09-01

    Full Text Available Plusieurs institutions d’enseignement supérieur ont adopté l’apprentissage par problèmes comme méthode pédagogique, mais peu d’études, à notre connaissance, se sont intéressées à décrire les stratégies utilisées par les étudiants dans un curriculum d’apprentissage par problèmes. Le but de cette recherche est justement l’étude de ces stratégies. L’analyse des verbatims obtenus auprès de 31 étudiantes1 de soins infirmiers a permis de dégager un ensemble de stratégies et d’en évaluer la fréquence. Les résultats suggèrent que les étudiantes utilisent davantage des stratégies de traitement en surface de l’information que des stratégies de traitement en profondeur malgré des indications voulant que l’utilisation d’une approche en profondeur soit associée à une meilleure réussite au cours.Several higher education institutions have adopted problem-based learning as an educational method. But, as far as we know, very few studies try to describe the strategies which are used by students in order to succeed in a problem-based learning context. The purpose of this research is to study those strategies. The analysis of the verbatim obtained from 31 nursing students uncovered the main strategies and their respective frequency. The results suggest that the students are more likely to use strategies based on surface treatment of the information than in-depth learning strategies. This preference is maintained even though they were told that in-depth learning strategies increase their academic achievements.

  7. Distinctive G Protein-Dependent Signaling by Protease-Activated Receptor 2 (PAR2 in Smooth Muscle: Feedback Inhibition of RhoA by cAMP-Independent PKA.

    Directory of Open Access Journals (Sweden)

    Wimolpak Sriwai

    Full Text Available We examined expression of protease-activated receptors 2 (PAR2 and characterized their signaling pathways in rabbit gastric muscle cells. The PAR2 activating peptide SLIGRL (PAR2-AP stimulated Gq, G13, Gi1, PI hydrolysis, and Rho kinase activity, and inhibited cAMP formation. Stimulation of PI hydrolysis was partly inhibited in cells expressing PAR2 siRNA, Gaq or Gai minigene and in cells treated with pertussis toxin, and augmented by expression of dominant negative regulator of G protein signaling (RGS4(N88S. Stimulation of Rho kinase activity was abolished by PAR-2 or Ga13 siRNA, and by Ga13 minigene. PAR2-AP induced a biphasic contraction; initial contraction was selectively blocked by the inhibitor of PI hydrolysis (U73122 or MLC kinase (ML-9, whereas sustained contraction was selectively blocked by the Rho kinase inhibitor (Y27632. PAR2-AP induced phosphorylation of MLC20, MYPT1 but not CPI-17. PAR2-AP also caused a decrease in the association of NF-kB and PKA catalytic subunit: the effect of PAR2-AP was blocked by PAR2 siRNA or phosphorylation-deficient RhoA (RhoA(S188A. PAR2-AP-induced degradation of IkBa and activation of NF-kB were abolished by the blockade of RhoA activity by Clostridium botulinum C3 exoenzyme suggesting RhoA-dependent activation of NF-kB. PAR2-AP-stimulated Rho kinase activity was significantly augmented by the inhibitors of PKA (myristoylated PKI, IKK2 (IKKIV or NF-kB (MG132, and in cells expressing dominant negative mutants of IKK (IKK(K44A, IkBa (IkBa (S32A/S36A or RhoA(S188A, suggesting feedback inhibition of Rho kinase activity via PKA derived from NF-kB pathway. PAR2-AP induced phosphorylation of RhoA and the phosphorylation was attenuated in cells expressing phosphorylation-deficient RhoA(S188A. Our results identified signaling pathways activated by PAR2 to mediate smooth muscle contraction and a novel pathway for feedback inhibition of PAR2-stimulated RhoA. The pathway involves activation of the NF-kB to

  8. The relationship between levels of plasma-soluble urokinase plasminogen activator receptor (suPAR) and presence of migraine attack and aura.

    Science.gov (United States)

    Yılmaz, Nigar; Yılmaz, Mustafa; Sirin, Burcu; Yılmaztekin, Sureyya; Kutlu, Gülnihal

    2017-10-01

    Migraine is one of the most common types of pain associated with sterile inflammatory conditions. Soluble urokinase plasminogen activator receptor (suPAR) is a potential novel inflammatory marker. We aim to determine the association between serum values of suPAR, procalcitonin, fibrinogen, and high-sensitivity C-reactive protein (hs-CRP) and migraine disease characteristics. The study involved a total of 60 migraine patients (33 patients in the interictal period, 27 patients in the attack period) and 30 healthy individuals. The serum values of suPAR were found to be significantly higher in migraine patients in the attack period than in migraine patients in the interictal period, and in healthy individuals (p migraine with aura patients than in migraine without aura patients. When we subdivided migraine patients according to frequency of attack (attacks/month), significant differences were found between the suPAR and procalcitonin levels (measured during the attack period) of those in the frequent-attack group (4-5 or more) versus those in the less frequent attack group (less than 4). Serum levels of procalcitonin were shown to be significantly higher in migraine patients during the attack period compared with migraine patients in the interictal period and in control subjects (p = .001 for both). Significant differences were found between plasma levels of fibrinogen in migraine patients versus control subjects (p migraine patients versus the control group. These findings may show that presenting a high level of suPAR in migraine patients with attack and aura results to predisposition to occurring on the symptoms and that high levels of suPAR, procalcitonin and fibrinogen in patients with migraine result in neurogenic inflammation during migraine headaches.

  9. Contribution to the studies on the mineral content of plant material through radioactivation analysis; Contribution a l'etude de la composition minerale des matieres vegetales au moyen de l'analyse par radioactivation

    Energy Technology Data Exchange (ETDEWEB)

    Fourcy, A. [Commissariat a l' Energie Atomique, Grenoble (France). Centre d' Etudes Nucleaires

    1968-03-15

    Radioactivation analysis is by its great sensibility or its rapidity quite helpful in plant biology and agronomy. Specific composition of plants and results to obtain in biological experimentation have needed a practical research on analytical methods for plant materials, using for radioactivation swimming-pool reactor neutrons and 14 MeV neutrons from a generator. Dosage process for 25 elements is exposed, taking account of the interest of the analysis for each element, the average amount occurring in plants and the result obtained. Many applications are developed, concerning nutrition physiology, genetics, parasitology, toxicology, control of manufactured agricultural and pharmaceutical products industrial and pesticides residues, ecology, radioecology and biochemistry. (author) [French] L'analyse par radioactivation, par sa grande sensibilite ou sa rapidite, est susceptible de rendre de nombreux services en biologie vegetale et en agronomie. La composition particuliere des plantes et les buts recherches dans l'experimentation ont exige une etude concrete des methodes d'analyse propres a la matiere vegetale en utilisant, pour la radioactivation, soit un reacteur de type piscine a eau legere, soit un accelerateur generateur de neutrons de 14 MeV. Le mode de dosage de 25 elements est expose en tenant compte de l'interet de l'analyse de chaque element, des teneurs moyennes rencontrees dans les plantes, et du resultat atteint. De nombreuses applications sont developpees qui touchent a la physiologie de la nutrition, la genetique, la parasitologie, la toxicologie, le controle des fabrications, les pollutions industrielles, l'ecologie, la radioecologie et la biochimie. (auteur)

  10. Contribution to the studies on the mineral content of plant material through radioactivation analysis; Contribution a l'etude de la composition minerale des matieres vegetales au moyen de l'analyse par radioactivation

    Energy Technology Data Exchange (ETDEWEB)

    Fourcy, A [Commissariat a l' Energie Atomique, Grenoble (France). Centre d' Etudes Nucleaires

    1968-03-15

    Radioactivation analysis is by its great sensibility or its rapidity quite helpful in plant biology and agronomy. Specific composition of plants and results to obtain in biological experimentation have needed a practical research on analytical methods for plant materials, using for radioactivation swimming-pool reactor neutrons and 14 MeV neutrons from a generator. Dosage process for 25 elements is exposed, taking account of the interest of the analysis for each element, the average amount occurring in plants and the result obtained. Many applications are developed, concerning nutrition physiology, genetics, parasitology, toxicology, control of manufactured agricultural and pharmaceutical products industrial and pesticides residues, ecology, radioecology and biochemistry. (author) [French] L'analyse par radioactivation, par sa grande sensibilite ou sa rapidite, est susceptible de rendre de nombreux services en biologie vegetale et en agronomie. La composition particuliere des plantes et les buts recherches dans l'experimentation ont exige une etude concrete des methodes d'analyse propres a la matiere vegetale en utilisant, pour la radioactivation, soit un reacteur de type piscine a eau legere, soit un accelerateur generateur de neutrons de 14 MeV. Le mode de dosage de 25 elements est expose en tenant compte de l'interet de l'analyse de chaque element, des teneurs moyennes rencontrees dans les plantes, et du resultat atteint. De nombreuses applications sont developpees qui touchent a la physiologie de la nutrition, la genetique, la parasitologie, la toxicologie, le controle des fabrications, les pollutions industrielles, l'ecologie, la radioecologie et la biochimie. (auteur)

  11. A flexible multidomain structure drives the function of the urokinase-type plasminogen activator receptor (uPAR)

    DEFF Research Database (Denmark)

    Mertens, Haydyn D.T.; Kjærgaard, Magnus; Mysling, Simon

    2012-01-01

    -deuterium exchange, and surface plasmon resonance to develop a structural model describing the allosteric regulation of uPAR. We show that the flexibility of its N-terminal domain provides the key for understanding this allosteric mechanism. Importantly, our model has direct implications for understanding uPAR-assisted...... cell adhesion and migration as well as for translational research including targeted intervention therapy and non-invasive tumor imaging in vivo....

  12. The Use of Short-Lived Radioactive Isotopes in an Activation Analysis Service Programme; Emploi des Radioisotopes a Courte Periode dans un Service d'Analyses par Activation; ИСПОЛЬЗОВАНИЕ КОРОТКОЖИВУПИХ РАДИОАКТИВНЫХ ИЗОТОПОВ В ПРОГРАММЕ АКТИВАЦИОННОГО АНАЛИЗА; Empleo de Radioisotopos de Periodo Corto en los Servicios de Analisis por Activacion

    Energy Technology Data Exchange (ETDEWEB)

    Gibbons, D.; Simpson, H. [Wantage Research Laboratory, Wantage (United Kingdom)

    1963-03-15

    also give an improved sensitivity. Several types of automatic units for use in counting short-lived radioisotopes are described, including photographic recording techniques, an automation sample changer and various programming units. (author) [French] Les radioisotopes a courte periode, notamment ceux dont la periode est egale ou inferieure a une heure, presentent divers avantages lorsqu'ils sont utilises pour l'analyse par activation. Parmi ces avantages on peut citer les suivants: sensibilite et selectivite (dans certains cas) plus favorables; temps de traitement total plus court, d'ou resultats plus rapides et souvent a meilleur compte; enfin, pour les analyses non destructives, possibilite de reactivation pour de nouvelles mesures. On surmonte progressivement les difficultes inherentes a des periodes aussi comtes. On a mis au point des methodes de separation radiochimique rapide telles que le traitement peut etre effectue sans qu'il se produise une desintegration radioactive excessive. L'emploi de spectrometres gamma perfectionnes et de methodes modernes de traitement des donnees a elargi le champ des analyses non destructives, reduisant ainsi la necessite de proceder a des purifications radiochimiques. Les auteurs illustrent les applications des radioisotopes a courte periode aux analyses par activation en decrivant les problemes d'analyse qu'ils ont eu effectivement a resoudre dans leur service d'analyse par activation. C'est ainsi que si l'on veut determiner la quantite de rubidium presente dans des sels de cesium, l'emploi de {sup 86m}Rb, dont la periode est de 1,0 min, reduit considerablement l'accumulation de l'activite matricielle pour une perte de sensibilite faible. Pour determiner le calcium present dans le tissu, c'est le {sup 49}Ca, dont la periode est de 8,8 min, qui donne les resultats le plus rapidement etant donne que la duree de l'irradiation est tres reduite et qu'en moins d'une heure on peut suivre la courbe de desintegration pour un nombre

  13. Electron paramagnetic resonance study of radicals formed by radiolysis at 77 K of nitroalkanes and of their solutions in organic glasses. Chromatography analysis of radiolysis products of nitromethane in ethanol solution in a vitreous medium; Etude par resonance paramagnetique electronique des radicaux formes par radiolyse a 77 K des nitroalcanes et de leurs solutions dans les verres organiques analyse par chromatographie des produits de la radiolyse du nitromethane en solution dans l'ethanol en milieux vitreux

    Energy Technology Data Exchange (ETDEWEB)

    Rosilio, C [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

    1968-05-01

    etude porte sur la structure et la reactivite des radicaux formes par irradiation 7 des derives nitres a 77 K, et sur le mecanisme de formation et de disparition de ces radicaux dans les differentes matrices utilisees. Les radicaux resultant de l'arrachement d'un hydrogene en {alpha} du groupe NO{sub 2}, et les radicaux resultant de reactions d'addition sur le groupe nitre caracterises par un electron non apparie sur l'azote ont ete identifies, soit dans la radiolyse des nitroalcanes purs, soit dans la radiolyse a 77 K des nitroalcanes en solution dans les verres organiques. La conformation et les mouvements des radicaux dans les matrices, le mecanisme de formation des radicaux observes resultant generalement de la capture par les nitroalcanes des especes primaires de la radiolyse ont ete etudies. Les nitroalcanes en solution dans l'ethanol se manifestent a la fois comme capteurs d'electrons et de radicaux libres. L'etude de la radiolyse des nitroalcanes en solution dans un verre polaire d'ethanol a ete completee par des analyses chimiques des produits finals de la radiolyse; elle nous a permis de deduire l'efficacite de capture des electrons pieges et des radicaux libres par les nitroalcanes dans l'ethanol. Pour cela nous avons dose, pour le systeme nitro-methane - ethanol les rendements radiochimiques de-l'hydrogene, de l'acetaldehyde et du glycol en fonction de la concentration en capteur. Un mecanisme de disparition des radicaux observes a ete propose. (auteur)

  14. Electron paramagnetic resonance study of radicals formed by radiolysis at 77 K of nitroalkanes and of their solutions in organic glasses. Chromatography analysis of radiolysis products of nitromethane in ethanol solution in a vitreous medium; Etude par resonance paramagnetique electronique des radicaux formes par radiolyse a 77 K des nitroalcanes et de leurs solutions dans les verres organiques analyse par chromatographie des produits de la radiolyse du nitromethane en solution dans l'ethanol en milieux vitreux

    Energy Technology Data Exchange (ETDEWEB)

    Rosilio, C. [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

    1968-05-01

    radiolyse. Notre etude porte sur la structure et la reactivite des radicaux formes par irradiation 7 des derives nitres a 77 K, et sur le mecanisme de formation et de disparition de ces radicaux dans les differentes matrices utilisees. Les radicaux resultant de l'arrachement d'un hydrogene en {alpha} du groupe NO{sub 2}, et les radicaux resultant de reactions d'addition sur le groupe nitre caracterises par un electron non apparie sur l'azote ont ete identifies, soit dans la radiolyse des nitroalcanes purs, soit dans la radiolyse a 77 K des nitroalcanes en solution dans les verres organiques. La conformation et les mouvements des radicaux dans les matrices, le mecanisme de formation des radicaux observes resultant generalement de la capture par les nitroalcanes des especes primaires de la radiolyse ont ete etudies. Les nitroalcanes en solution dans l'ethanol se manifestent a la fois comme capteurs d'electrons et de radicaux libres. L'etude de la radiolyse des nitroalcanes en solution dans un verre polaire d'ethanol a ete completee par des analyses chimiques des produits finals de la radiolyse; elle nous a permis de deduire l'efficacite de capture des electrons pieges et des radicaux libres par les nitroalcanes dans l'ethanol. Pour cela nous avons dose, pour le systeme nitro-methane - ethanol les rendements radiochimiques de-l'hydrogene, de l'acetaldehyde et du glycol en fonction de la concentration en capteur. Un mecanisme de disparition des radicaux observes a ete propose. (auteur)

  15. Processus d'apprentissage a distance et teleconference assistee par ordinateur: essai d'analyse (The Process of Distance Learning and Teleconferencing Assisted by Computer: An Analytic Essay).

    Science.gov (United States)

    Henri, France

    1992-01-01

    Discusses the distance learning process that occurs when computer-mediated communication is used and presents a conceptual framework based on the theoretical principles developed by Gavriel Salomon. Characteristics of the technology are identified, including active user participation, activation of cognitive skills related to information…

  16. Psychometric analyses to improve the Dutch ICF Activity Inventory

    NARCIS (Netherlands)

    Bruijning, J.E.; van Rens, G.H.M.B.; Knol, D.L.; van Nispen, R.M.A.

    2013-01-01

    PURPOSE: In the past, rehabilitation centers for the visually impaired used unstructured or semistructured methods to assess rehabilitation needs of their patients. Recently, an extensive instrument, the Dutch ICF Activity Inventory (D-AI), was developed to systematically investigate rehabilitation

  17. Analytical procedures used by the uranium - radon - radium geochemistry group; Methodes d'analyses utilisees par la section de geochimie uranium, radon, radium

    Energy Technology Data Exchange (ETDEWEB)

    Berthollet, P [Commissariat a l' Energie Atomique, Fontenay-aux-Roses (France). Centre d' Etudes Nucleaires

    1968-07-01

    The analytical methods described are applied to the geochemical prospecting of uranium. The nature of the material under investigation, which may be soil, alluvium, rock, plant or water, and the particular requirements of geochemical exploration, have prompted us to adjust the widely used conventional methods to the demands of large scale operation, without lowering their standards of accuracy and reliability. These procedures are explained in great detail. Though most of this technical information may appear superfluous to the chemical engineer well versed in trace element determination, it will, however, serve a useful purpose both with the operator in charge of routine testing and with the chemist called upon to interpret results. (author) [French] Les methodes d'analyses decrites sont utilisees pour la prospection geochimique de l'uranium. La nature des materiaux: sols, alluvions, roches, vegetaux, eaux, et les exigences propres a la prospection geochimique, nous ont conduit a adapter des methodes classique couramment utilisees pour les rendre aptes a etre executees en grande serie, sans abandonner leurs qualites de precision et de fidelite. Ces methodes sont presentees avec un maximum de details operatoires qui paraitront superflus aux chimistes habitues aux dosages de traces, mais seront utiles aussi bien aux manipulateurs charges des analyses qu'aux geochimistes appeles a exploiter les resultats. (auteur)

  18. The pro-inflammatory biomarker soluble urokinase plasminogen activator receptor (suPAR) is associated with incident type 2 diabetes among overweight but not obese individuals with impaired glucose regulation

    DEFF Research Database (Denmark)

    Heraclides, A; Jensen, T M; Rasmussen, S S

    2013-01-01

    weight status and suPAR were tested. During a 3-year follow-up (599 incident diabetes cases), there was a 48% overall increase in the odds of developing type 2 diabetes per twofold increase in suPAR (p = 0.006). This association was modified by body weight status in overweight, but not in obese...... among overweight participants. suPAR may be a good novel biomarker for systemic sub-clinical inflammation and immune activation linked to incident type 2 diabetes risk in overweight individuals and non-smokers. The observed interactions with adiposity and smoking should be investigated further.......Recent evidence links the soluble urokinase plasminogen activator receptor (suPAR), a stable biomarker of systemic immune activation, to several chronic diseases, including type 2 diabetes. suPAR is also associated with adiposity and smoking. We hypothesised that this biomarker would be linked...

  19. Directed and persistent movement arises from mechanochemistry of the ParA/ParB system.

    Science.gov (United States)

    Hu, Longhua; Vecchiarelli, Anthony G; Mizuuchi, Kiyoshi; Neuman, Keir C; Liu, Jian

    2015-12-22

    The segregation of DNA before cell division is essential for faithful genetic inheritance. In many bacteria, segregation of low-copy number plasmids involves an active partition system composed of a nonspecific DNA-binding ATPase, ParA, and its stimulator protein ParB. The ParA/ParB system drives directed and persistent movement of DNA cargo both in vivo and in vitro. Filament-based models akin to actin/microtubule-driven motility were proposed for plasmid segregation mediated by ParA. Recent experiments challenge this view and suggest that ParA/ParB system motility is driven by a diffusion ratchet mechanism in which ParB-coated plasmid both creates and follows a ParA gradient on the nucleoid surface. However, the detailed mechanism of ParA/ParB-mediated directed and persistent movement remains unknown. Here, we develop a theoretical model describing ParA/ParB-mediated motility. We show that the ParA/ParB system can work as a Brownian ratchet, which effectively couples the ATPase-dependent cycling of ParA-nucleoid affinity to the motion of the ParB-bound cargo. Paradoxically, this resulting processive motion relies on quenching diffusive plasmid motion through a large number of transient ParA/ParB-mediated tethers to the nucleoid surface. Our work thus sheds light on an emergent phenomenon in which nonmotor proteins work collectively via mechanochemical coupling to propel cargos-an ingenious solution shaped by evolution to cope with the lack of processive motor proteins in bacteria.

  20. Gamma-activation analyses of 187 Os in molybdenite

    International Nuclear Information System (INIS)

    Dikij, N.P.; Dovbnya, A.N.; Borovlev, V.I.; Lyashko, Yu.V.; Medvedeva, E.P.; Uvarov, V.L.; Val'ter, A.A.; Storizhko, V.E.

    1999-01-01

    197 Os and 187 Re are used in cosmological studies. Common registration of gamma and X-ray irradiation in compositions containing these radioisotopes permits to increase the accuracy in determination of their content. The results of 187 Os gamma-activation analysis in molybdenite are presented

  1. Analyse de l'état mécanique et microstructural de films minces supraconducteurs YBa_2 Cu_3O_7 par diffraction des rayons X

    Science.gov (United States)

    Auzary, S.; Badawi, K. F.; Bimbault, L.; Rabier, J.; Gaboriaud, R. J.; Goudeau, Ph.

    1997-01-01

    Mechanical and microstructural analysis in a 100nm thin film is presented in this study. Using X-ray diffraction with a tensorial approach, we have determined stresses, strains, stress-free lattice parameters, microdistorsions and diffracting coherent domains size. Stress-free lattice parameters are higher than the bulk values. A high value of stresses is explained as a combination of coherent stresses, thermal stresses and intrinsic ones. Diffraction peaks line profiles analysis suggests grain boundaries presence as well as high lattice elastic microdistorsions. Cette étude présente une analyse de l'état mécanique et microstructurale dans un film mince de 100nm d'épaisseur d'YBCO déposé sur un substrat de MgO. En utilisant la diffraction des rayons X couplée à une approche tensorielle, nous avons déterminé les déformations, les contraintes, les paramètres libres de contraintes, les microdistorsions élastiques ainsi que la taille des domaines cohérents de diffraction. Les paramètres libres de contrainte sont supérieurs à ceux du massif. Une valeur élevée des contraintes est expliquée à partir des contraintes de cohérence, des contraintes thermiques et intrinsèques. L'analyse des profils des pics de diffraction suggère la présence de sous-joints et de distorsions élastiques élevées au niveau des mailles cristallographiques.

  2. Dans les ramures de l’arbre hypertexte: Analyse des incitations générées par l’opacité du moteur Google

    NARCIS (Netherlands)

    Sire, G.; Rieder, B.

    2015-01-01

    Does Google have incentives to bias the treatment of information? With Google’s numerous areas of activity, we intend to demonstrate that it would have the possibility to distort both the advertising and the information market. Google’s way of working is largely opaque and it is difficult to get to

  3. The Byzantine ceramics from Pergamon excavations. Characterization of local and imported productions by elementary analysis using PIXE and INAA methods and by petrography; Les ceramiques byzantines des fouilles de Pergame. Caracterisation des productions locales et importees par analyse elementaire par les methodes PIXE et INAA et par petrographie

    Energy Technology Data Exchange (ETDEWEB)

    Waksman, S Y

    1995-01-12

    An important ceramics material dated back to the 12th-14th centuries has been excavated in Pergamon (Turkey). Among these findings, wasters, tripod stilts and unfinished ware attest to local production in the Byzantine period. Elemental analysis by the methods PIXE (Particle Induced X-ray Emission) and INAA (Instrumental Neutron Activation Analysis) has been performed on a representative sampling of 160 sherds, including attested local material. Multivariate statistical techniques were used to classify the sherds into groups of similar composition and thus to distinguish ceramics made in Pergamon from imported wares. Several groups of local production have been constituted, which correspond to wares differing in date and fabric. The geochemical characterization of the pastes, complemented with petrographical and mineralogical data, shows that specific raw materials have been used to manufacture each ware. The analytical data related to ceramics made in Pergamon will serve as reference data for future provenance studies. Such reference groups of Byzantine ceramics are very rare, and therefore the ceramics imported into Pergamon cannot be attributed as to their origin. Among the ceramics widely diffused in the Byzantine world, some importations belonging to the ``fine sgraffito`` and ``Zeuxippus ware`` types have been identified. The latter type has been a source of stylistic influence for the workshops of Pergamon, since the analyses show that imitated ``Zeuxippus ware`` has been produced there. These imitations were probably themselves diffused on a regional scale. (author). 238 refs., 48 figs., 53 tabs., 22 photos., 8 appends.

  4. Analyse des kérosènes et des gas oils moyens par spectrométrie de masse à moyenne résolution Analysis of Kerosenes and Middle Distillates by Medium-Resolution Mass Spectrometry

    Directory of Open Access Journals (Sweden)

    Castex H.

    2006-11-01

    Full Text Available Une méthode d'analyse par spectrométrie de masse des kérosènes et des distillats moyens dérivée de la norme ASTM D2425, a été développée. Elle utilise le même principe de calcul du carbone moyen pour déterminer le choix des matrices appropriées. Mais elle opère à moyenne résolution, effectuant ainsi le tri des différentes masses exactes d'hydrocarbures, composés soufrés et oxygénés et évitant de ce fait toute séparation préalable par chromatographie liquide (ASTM D2 549 . Les valeurs des matrices ont été modifiées en conséquence et le calcul du carbone moyen adapté à l'étude des coupes étroites et larges. A mass-spectrometry analysis method derived from the ASTM D2425 standard has been developed for kerosenes and middle distillates (185-345°C. It uses the same principle for calculating the mean carbon value to determine the appropriate matrices. But it works with medium reso-lution so as to sort out the different exact masses of hydrocarbons, sulfur and oxygenated compounds, thus avoiding any prior separation by liquid chromatography (ASTM D2549. Therefore, the matrix values have been modified, and the calculation of the mean carbon value has been adapted for analyzing narrow and wide distillation cuts.

  5. Assessment of tissue-specific cortisol activity with regard to degeneration of the suspensory ligaments in horses with pituitary pars intermedia dysfunction.

    Science.gov (United States)

    Hofberger, Sina C; Gauff, Felicia; Thaller, Denise; Morgan, Ruth; Keen, John A; Licka, Theresia F

    2018-02-01

    OBJECTIVE To identify signs of tissue-specific cortisol activity in samples of suspensory ligament (SL) and neck skin tissue from horses with and without pituitary pars intermedia dysfunction (PPID). SAMPLE Suspensory ligament and neck skin tissue samples obtained from 26 euthanized horses with and without PPID. PROCEDURES Tissue samples were collected from 12 horses with and 14 horses without PPID (controls). Two control horses had received treatment with dexamethasone; data from those horses were not used in statistical analyses. The other 12 control horses were classified as old horses (≥ 14 years old) and young horses (≤ 9 years old). Standard histologic staining, staining for proteoglycan accumulation, and immunostaining of SL and neck skin tissue sections for glucocorticoid receptors, insulin, 11β hydroxysteroid dehydrogenase type 1, and 11β hydroxysteroid dehydrogenase type 2 were performed. Findings for horses with PPID were compared with findings for young and old horses without PPID. RESULTS Compared with findings for old and young control horses, there were significantly more cells stained for glucocorticoid receptors in SL samples and for 11 β hydroxysteroid dehydrogenase type 1 in SL and skin tissue samples from horses with PPID. Insulin could not be detected in any of the SL or skin tissue samples. Horses with PPID had evidence of SL degeneration with significantly increased proteoglycan accumulation. Neck skin tissue was found to be significantly thinner in PPID-affected horses than in young control horses. CONCLUSIONS AND CLINICAL RELEVANCE Results suggested that tissue-specific dysregulation of cortisol metabolism may contribute to the SL degeneration associated with PPID in horses.

  6. Absolute analysis of uranium isotopic concentrations with a gas ion source mass spectrometer; Analyses absolues des concentrations isotopiques de l'uranium par spectrometre de masse equipe d'une source a gaz

    Energy Technology Data Exchange (ETDEWEB)

    Chaussy, L.; Boyer, R. [Commissariat a l' Energie Atomique, Pierrelatte (France)

    1969-07-01

    Mass spectrometer with electronic bombardment ions source for routine uranium isotopic analysis are used like relative measurements apparatus. We show that such mass spectrometers can be used for absolute measurements with a very high sensitivity and precision which are ten times better than theses of thermo-ionic ions source mass spectrometer. We examine the causes of systematic errors and we give experimental data. In particular natural uranium sample used as reference give: U{sub 5} = 0.7202 {+-} 0.0005 atoms per cent; U{sub 4} = 0.00552 {+-} 0.0003 atoms per cent. The use of this method is justified for standards control. (authors) [French] Les spectrometres de masse a source par bombardement electronique pour l'analyse de l'uranium sous forme d'hexafluorure, sont utilises en routine comme des appareils de mesure relative. On montre que l'on peut utiliser de tels appareils pour effectuer des mesures absolues avec une excellente sensibilite et reproductibilite, dix fois superieure a celle des spectrometres a source thermoionique. On examine en detail les causes d'erreurs systematiques et on donne des resultats experimentaux. En particulier, l'analyse d'un echantillon d'uranium naturel donne: U{sub 5} = 0.7202 {+-} 0.0005 atomes pour cent; U{sub 4} = 0.00552 {+-} 0.0003 atomes pour cent. La technique de mesure est utile pour le controle d'etalons isotopiques. (auteurs)

  7. Monitoring of Volcanic Activity by Sub-mm Geodetic Analyses

    Science.gov (United States)

    Miura, S.; Mare, Y.; Ichiki, M.; Demachi, T.; Tachibana, K.; Nishimura, T.

    2017-12-01

    Volcanic earthquakes have been occurring beneath Zao volcano in northern Honshu, Japan since 2013, following the increase of deep low frequency earthquakes from 2012. On account of a burst of seismicity initiated in April 2015, the JMA announced a warning of eruption, however, the seismicity gradually decreased for the next two months and the warning was canceled in June. In the same time period, minor expansive deformation was observed by GNSS. Small earthquakes are still occurring, and low-freq. earthquakes (LPE) occur sometimes accompanied by static tilt changes. In this study, we try to extract the sub-mm displacements from the LPE waveforms observed by broadband seismometers (BBS) and utilize them for geodetic inversion to monitor volcanic activities. Thun et al. (2015, 2016) devised an efficient method using a running median filter (RMF) to remove LP noises, which contaminate displacement waveforms. They demonstrated the reproducibility of the waveforms corresponding to the experimentally given sub-mm displacements in the laboratory. They also apply the method to the field LPE data obtained from several volcanoes to show static displacements. The procedure is outlined as follows: (1) Unfiltered removal of the instrument response, (2) LP noise estimate by LPF with a corner frequency of 5/M, where M (seconds) is the time window of the RMF and should be at least three times the length of the rise time. (3) Subtract the noise estimated from step (2). (4) Integrate to obtain displacement waveforms. We apply the method to the BBS waveform at a distance of about 1.5 km ESE from the summit crater of Zao Volcano associated with a LPE on April 1, 2017. Assuming the time window M as 300 seconds, we successfully obtained the displacement history: taking the rise time of about 2 minutes, the site was gradually uplifted with the amount of about 50-60 µm and then subsided with HF displacements in the next 2 minutes resulting about 20-30 µm static upheaval. Comparing the

  8. Analyses of atmospheric radon 222 / canisters exposed by Greenpeace in Niger (Arlit / Akokan sector); Analyses du radon 222 dans l'air ambiant / Capteurs exposes par Greenpeace au Niger (secteur Arlit et Akokan)

    Energy Technology Data Exchange (ETDEWEB)

    Chareyron, B.

    2010-07-01

    The companies SOMAIR and COMINAK, subsidiaries of the AREVA group, are mining uranium deposits in northern Niger. In the course of a field mission carried out in November 2009, a Greenpeace International team deposited detectors (canisters of activated charcoal) to measure radon 222, a radioactive gas formed by the decay of the radium 226 present in the uranium ore. This report includes the results of the analysis of the activated charcoal canisters conducted in CRIIRAD's laboratory, and a brief commentary on the interpretation of the results. (authors)

  9. suPAR

    DEFF Research Database (Denmark)

    Eugen-Olsen, Jesper; Giamarellos-Bourboulis, Evangelos J

    2015-01-01

    Investigation of biomarkers that can promptly predict unfavourable outcome of critically illness is an emerging necessity taking into consideration the need for early intervention, the shortage of available beds in intensive care units and the considerable cost of hospitalisation. The most...... promising biomarker is soluble urokinase-type plasminogen activator receptor (suPAR). Three studies in large populations of critically ill patients and patients admitted to the emergency department have shown that concentrations >12ng/mL can safely predict unfavourable outcome. This review presents...

  10. Perturbation analysis of cyclotron resonance in the electromagnetic field of a TE{sub 011} mode; Analyse par perturbation de la resonance cyclotronique dans le champ electromagnetique en mode TE{sub 011} mode

    Energy Technology Data Exchange (ETDEWEB)

    Dreicer, H [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

    1968-07-01

    The motion of an electron orbiting under the combined action of a static magnetic field and the AC azimuthal electric field of a cylindrical TE{sub 011} mode is analyzed with help of a perturbation technique. The first and second order perturbation results indicate that at cyclotron resonance the electron's center of gyration oscillates slowly at right angles to the magnetic field between two turning points. We find that superimposed upon this nearly static Exb drift the electron cyclically undergoes the process of cyclotron absorption and induced emission. Our results indicate that it is possible to insure maser action (i.e. induced emission rather than absorption) without special preparation of the electron's velocity provided that the electron is introduced into the field in certain special regions of space pervaded by the TE mode. This is a case where over-population of the upper state is accomplished through 'pumping' in real space. The relation between an electron cyclotron resonance maser based upon this principle and one based upon the principle of velocity space pumping, due to Twiss, is examined. This treatment provides physical interpretations and verifies the numerical results found earlier by Le Gardeur. (author) [French] Le mouvement d'un electron soumis a l'action combinee d'un champ magnetique statique et d'un champ electrique haute frequence azimutal engendre dans une cavite cylindrique en mode TE{sub 011} est analyse a partir d'une methode de perturbation. Les resultats des perturbations au premier et deuxieme ordre indiquent qu'a la resonance cyclotronique, le centre de giration de l'electron oscille lentement dans le plan perpendiculaire au champ magnetique entre deux points de rebroussement. En plus de la derivee quasi-statique ExB, l'electron passe par des etats d'absorption et emission cyclotronique. Les resultats du calcul confirment la possibilite d'avoir une action maser (c'est-a-dire: emission au lieu d'absorption) sans que la vitesse des

  11. Dresses problems arising from hot-plant operation and their solution (1961); Les problemes vestimentaires poses par l'exploitation des installations actives et leurs solutions (1961)

    Energy Technology Data Exchange (ETDEWEB)

    Rodier, J; Bouzigues, H; Boutot, P [Commissariat a l' Energie Atomique, Centre de Production de Plutonium, Marcoule (France). Centre d' Etudes Nucleaires

    1961-07-01

    This article deals with effective methods to struggle radioactive contamination using rationally designed working clothes. The choice of the cloth is important and cotton, because of its absorbent properties constitutes an effective barrier to radioelements failing on its surface. Clothing the personnel of large nuclear industrial concerns is a big problem which can only be solved by carefully studied methods. The decontamination and washing of large amounts of clothes whose flow increases during periods of radio-active incidents have to be treated as are those operations in hospital laundering. Linen washing by the German counter-current method (the Sulzman system) is of great value because of the volume which can be treated and, more important, because of the small amount of liquid waste produced. Dry cleaning is certainly a method of the future and is even more economic than the preceding one. In 'active' laundries, the control of clothing for residual contamination can constitute a serious bottleneck in the production of clean clothing if automatic high-speed machines are not used. The risk to the operating personnel comes solely from contamination of the atmosphere and of the surfaces. Because of this, the plant must be considered as an active zone in which are handled several tens of millicuries of dangerous emitters and several micro- curies of plutonium daily. (authors) [French] Ce memoire traite des moyens efficaces de lutte contre la contamination radioactive que peuvent offrir des vetements de travail de conception rationnelle. Le choix du tissu est un element important et la fibre de coton, par ses proprietes absorbantes, constitue un excellent barrage aux radioelements deposes a sa surface. L'habillement du personnel des grands ensembles industriels de l'energie atomique est un gros probleme qui ne peut etre solutionne qu'avec des moyens soigneusement etudies. La decontamination et le lavage des grandes quantites de vetements renouveles a cadence

  12. Dresses problems arising from hot-plant operation and their solution (1961); Les problemes vestimentaires poses par l'exploitation des installations actives et leurs solutions (1961)

    Energy Technology Data Exchange (ETDEWEB)

    Rodier, J.; Bouzigues, H.; Boutot, P. [Commissariat a l' Energie Atomique, Centre de Production de Plutonium, Marcoule (France). Centre d' Etudes Nucleaires

    1961-07-01

    This article deals with effective methods to struggle radioactive contamination using rationally designed working clothes. The choice of the cloth is important and cotton, because of its absorbent properties constitutes an effective barrier to radioelements failing on its surface. Clothing the personnel of large nuclear industrial concerns is a big problem which can only be solved by carefully studied methods. The decontamination and washing of large amounts of clothes whose flow increases during periods of radio-active incidents have to be treated as are those operations in hospital laundering. Linen washing by the German counter-current method (the Sulzman system) is of great value because of the volume which can be treated and, more important, because of the small amount of liquid waste produced. Dry cleaning is certainly a method of the future and is even more economic than the preceding one. In 'active' laundries, the control of clothing for residual contamination can constitute a serious bottleneck in the production of clean clothing if automatic high-speed machines are not used. The risk to the operating personnel comes solely from contamination of the atmosphere and of the surfaces. Because of this, the plant must be considered as an active zone in which are handled several tens of millicuries of dangerous emitters and several micro- curies of plutonium daily. (authors) [French] Ce memoire traite des moyens efficaces de lutte contre la contamination radioactive que peuvent offrir des vetements de travail de conception rationnelle. Le choix du tissu est un element important et la fibre de coton, par ses proprietes absorbantes, constitue un excellent barrage aux radioelements deposes a sa surface. L'habillement du personnel des grands ensembles industriels de l'energie atomique est un gros probleme qui ne peut etre solutionne qu'avec des moyens soigneusement etudies. La decontamination et le lavage des grandes quantites de vetements

  13. Hypersensitivity Induced by Activation of Spinal Cord PAR2 Receptors Is Partially Mediated by TRPV1 Receptors

    Czech Academy of Sciences Publication Activity Database

    Mrózková, Petra; Špicarová, Diana; Paleček, Jiří

    2016-01-01

    Roč. 11, č. 10 (2016), č. článku e0163991. E-ISSN 1932-6203 R&D Projects: GA ČR(CZ) GA15-11138S; GA MŠk(CZ) LH15279; GA ČR(CZ) GBP304/12/G069; GA MŠk(CZ) ED1.1.00/02.0109 Institutional support: RVO:67985823 Keywords : PAR2 * TRP * neuropathy * spinal cord Subject RIV: FH - Neurology Impact factor: 2.806, year: 2016

  14. Gas Well Testing Analysis Using Finite-Difference Models and Optimization Techniques Analyse d'essais de puits de gaz par des modèles utilisant la méthode des différences finies et par des techniques d'optimisation

    Directory of Open Access Journals (Sweden)

    Darderes E. A.

    2006-11-01

    paramètres à partir d'essais de puits de gaz. Une des plus performantes est l'analyse du régime pseudo-permanent pour lequel on trouve, sur un graphique semi-logarithmique, une relation linéaire entre la pseudo-pression d'un gaz réel et le temps. Une autre méthode est l'ajustement à des courbes types. Les deux méthodes présentent l'inconvénient de ne pouvoir être rigoureusement appliquées qu'à des cas spécifiques. Cet article expose une méthode générale permettant d'analyser les données des essais de puits par ajustement automatisé des courbes types. Elle est basée sur le meilleur accord d'un modèle numérique avec les données obtenues dans les essais de puits de gaz grâce à des techniques d'optimisation. Le modèle numérique fournit la solution d'une équation différentielle à dérivées partielles du 2e ordre et fortement non linéaire qui régit le flux radial des gaz réels vers un puits de production. L'équation différentielle est résolue par la méthode des différences finies en tenant compte des variations des propriétés des gaz avec la pression. Pour simuler n'importe quel type d'essais de puits de gaz, on impose des conditions initiales et aux limites appropriées. On minimise les différences résiduelles entre les pressions réelles mesurées pendant l'essai et les pressions calculées par la méthode des moindres carrés. On utilise deux techniques d'optimisation pour obtenir la meilleure estimation des paramètres qui minimisent la somme des carrés des résidus : la méthode monovariante de Fibonacci [1] et la méthode polyvariante de Marquardt [1, 2]. On applique ce mode opératoire à des essais de puits menés à un débit constant et à deux débits constants. Les résultats numériques concordent très bien avec les données des essais de puits.

  15. uPAR Expression Pattern in Patients with Urothelial Carcinoma of the Bladder

    DEFF Research Database (Denmark)

    Dohn, Line Hammer; Pappot, Helle; Iversen, Benedikte Richter

    2015-01-01

    The objective of the present study was to confirm the expression and localisation pattern of the urokinase-type plasminogen activator receptor (uPAR) focusing on its possible clinical relevance in patients with urothelial neoplasia of the bladder. uPAR is a central molecule in tissue remodelling...... during cancer invasion and metastasis and is an established prognostic marker in various cancer diseases other than bladder cancer. Formalin-fixed and paraffin-embedded tumour-tissue blocks from 186 patients treated with radical cystectomy were analysed. uPAR expression was scored as either negative...... or positive as well as by the actual score. Separate scores were obtained for cancer cells, macrophages and myofibroblasts at the invasive front and in tumour core. We were able to confirm, in an independent patient cohort, the tissue expression and localisation pattern of uPAR as investigated...

  16. Monitoring of chemotherapy successfulness of Platina/Taxol chemotherapy protocol by using determination of serum urokinase plasminogen activator (uPA and soluble urokinase plasminogen activator receptor (suPAR in patients with ovarian carcinoma FIGO II

    Directory of Open Access Journals (Sweden)

    Dženita Ljuca

    2007-05-01

    Full Text Available In about 70% of cases, ovarian carcinoma has been diagnosed at an advanced stage. Invasion and metastasis of solid tumors request protease activity resulting in basal membrane destruction and surrounding matrix. In that process, urokinase plasminogen activator (uPA and its receptor, urokinase plasminogen activator receptor (suPAR play a key role, that via plasmin activation lead to basal membrane and matrix degradation in surrounding of the tumor, enable to its invasion and metastasis. Determination of serum concentration of those tumor markers can be useful in preoperative as well as in postoperative period. Their serum concentrations in ovarian cancer patients may help in good monitoring of remission or progression during chemotherapy treatment. In late 1950s and eariy 1960s, when it was found out that malignant ovarian tumors were chemosensitive, their chemotherapy treatment has begun. In the beginning it was used only mono-therapy, and by discovering new cytostatics it was replaced by poly-chemotherapy. Now days, in the therapy of advanced stages of ovarian carcinoma combination of cisplatine or carboplatine with paclitaxel is considering as standard treatment. Aim of this study was to determine serum uPA, suPAR and CEA in FIGO II and III patients with different histo-logical type (serous, mucinous, clear cell tumor before and after PT chemotherapy protocol during following three cycles. In this prospective study we have analyzed 17 patients with ovarian carcinoma, those have been after surgery treated by chemotherapy. Serum levels of uPA and suPAR have been determined by ELISA-test (Imubind uPA, Imubind uPAR, American Diagnostica, and CEA by OPUS Imunoassay method. Results of this study have shown that uPA, suPAR and CEA met criteria for prognostic markers for monitoring of successful-ness of platina/taxol chemotherapy protocol for serous, mucinous and clear cell tumor FIGO II and III stage of ovarian carcinoma. In case of PT chemotherapy

  17. Age and gender effects on normal regional cerebral blood flow studied using two different voxel-based statistical analyses; Effets de l'age et du genre sur la perfusion cerebrale regionale etudiee par deux methodes d'analyse statistique voxel-par-voxel

    Energy Technology Data Exchange (ETDEWEB)

    Pirson, A.S.; George, J.; Krug, B.; Vander Borght, T. [Universite Catholique de Louvain, Service de Medecine Nucleaire, Cliniques Universitaires de Mont-Godinne, Yvoir (Belgium); Van Laere, K. [Leuven Univ. Hospital, Nuclear Medicine Div. (Belgium); Jamart, J. [Universite Catholique de Louvain, Dept. de Biostatistiques, Cliniques Universitaires de Mont-Godinne, Yvoir (Belgium); D' Asseler, Y. [Ghent Univ., Medical Signal and Image Processing Dept. (MEDISIP), Faculty of applied sciences (Belgium); Minoshima, S. [Washington Univ., Dept. of Radiology, Seattle (United States)

    2009-10-15

    Fully automated analysis programs have been applied more and more to aid for the reading of regional cerebral blood flow SPECT study. They are increasingly based on the comparison of the patient study with a normal database. In this study, we evaluate the ability of Three-Dimensional Stereotactic Surface Projection (3 D-S.S.P.) to isolate effects of age and gender in a previously studied normal population. The results were also compared with those obtained using Statistical Parametric Mapping (S.P.M.99). Methods Eighty-nine {sup 99m}Tc-E.C.D.-SPECT studies performed in carefully screened healthy volunteers (46 females, 43 males; age 20 - 81 years) were analysed using 3 D-S.S.P.. A multivariate analysis based on the general linear model was performed with regions as intra-subject factor, gender as inter-subject factor and age as co-variate. Results Both age and gender had a significant interaction effect with regional tracer uptake. An age-related decline (p < 0.001) was found in the anterior cingulate gyrus, left frontal association cortex and left insula. Bilateral occipital association and left primary visual cortical uptake showed a significant relative increase with age (p < 0.001). Concerning the gender effect, women showed higher uptake (p < 0.01) in the parietal and right sensorimotor cortices. An age by gender interaction (p < 0.01) was only found in the left medial frontal cortex. The results were consistent with those obtained with S.P.M.99. Conclusion 3 D-S.S.P. analysis of normal r.C.B.F. variability is consistent with the literature and other automated voxel-based techniques, which highlight the effects of both age and gender. (authors)

  18. Altered Brain Activity in Unipolar Depression Revisited: Meta-analyses of Neuroimaging Studies.

    Science.gov (United States)

    Müller, Veronika I; Cieslik, Edna C; Serbanescu, Ilinca; Laird, Angela R; Fox, Peter T; Eickhoff, Simon B

    2017-01-01

    During the past 20 years, numerous neuroimaging experiments have investigated aberrant brain activation during cognitive and emotional processing in patients with unipolar depression (UD). The results of those investigations, however, vary considerably; moreover, previous meta-analyses also yielded inconsistent findings. To readdress aberrant brain activation in UD as evidenced by neuroimaging experiments on cognitive and/or emotional processing. Neuroimaging experiments published from January 1, 1997, to October 1, 2015, were identified by a literature search of PubMed, Web of Science, and Google Scholar using different combinations of the terms fMRI (functional magnetic resonance imaging), PET (positron emission tomography), neural, major depression, depression, major depressive disorder, unipolar depression, dysthymia, emotion, emotional, affective, cognitive, task, memory, working memory, inhibition, control, n-back, and Stroop. Neuroimaging experiments (using fMRI or PET) reporting whole-brain results of group comparisons between adults with UD and healthy control individuals as coordinates in a standard anatomic reference space and using an emotional or/and cognitive challenging task were selected. Coordinates reported to show significant activation differences between UD and healthy controls during emotional or cognitive processing were extracted. By using the revised activation likelihood estimation algorithm, different meta-analyses were calculated. Meta-analyses tested for brain regions consistently found to show aberrant brain activation in UD compared with controls. Analyses were calculated across all emotional processing experiments, all cognitive processing experiments, positive emotion processing, negative emotion processing, experiments using emotional face stimuli, experiments with a sex discrimination task, and memory processing. All meta-analyses were calculated across experiments independent of reporting an increase or decrease of activity in

  19. Application of the IEAF-2001 activation data library to activation analyses of the IFMIF high flux test module

    International Nuclear Information System (INIS)

    Fischer, U.; Wilson, P.P.H.; Leichtle, D.; Simakov, S.P.; Moellendorff, U. von; Konobeev, A.; Korovin, Yu.; Pereslavtsev, P.; Schmuck, I.

    2002-01-01

    A complete activation data library IEAF-2001 (intermediate energy activation file) has been developed in standard ENDF-6 format with neutron-induced activation cross sections for 679 target nuclides from Z=1 (hydrogen) to Z=84 (polonium) and incident neutron energies up to 150 MeV. Using the NJOY processing code, an IEAF-2001 working library has been prepared in a 256 energy group structure for enabling activation analyses of the International Fusion Material Irradiation Facility (IFMIF) D-Li neutron source. This library was applied to the activation analysis of the IFMIF high flux test module using the recent Analytical and Laplacian Adaptive Radioactivity Analysis activation code which is capable of handling the variety of reaction channels open in the energy domain above 20 MeV. The IEAF-2001 activation library was thus shown to be suitable for activation analyses in fusion technology and intermediate energy applications such as the IFMIF D-Li neutron source

  20. Cathepsin S Is Involved in Th17 Differentiation Through the Upregulation of IL-6 by Activating PAR-2 after Systemic Exposure to Lipopolysaccharide from Porphyromonas gingivalis

    Directory of Open Access Journals (Sweden)

    Masato Dekita

    2017-07-01

    Full Text Available Positive links have been found between periodontitis and numerous diseases in humans via persistent inflammation throughout the body. However, the main factors responsible for maintaining this pro-inflammatory condition are poorly understood. The spleen, the largest secondary immune organ, is a central hub regulating the immune response/inflammation due to the dendritic cell (DC response to CD4+ T cell subtype differentiation, and lysosomal proteinase cathepsin S (CatS is known to be involved in DC functions. In the present study, we found that CatS-induced IL-6 production by splenic DCs subsequently promotes Th17 differentiation, in response to systemic exposure to lipopolysaccharide derived from Porphyromonas gingivalis (PgLPS. The population of CD11c+ DCs was significantly increased in the splenic marginal zone (MZ locally of wild-type (DBA/2 mice with splenomegaly but not in that of CatS deficient (CatS-/- mice after systemic exposure to PgLPS for 7 consecutive days (5 mg/kg/day, intraperitoneal. Similarly, the population of Th17+CD4+ T cells was also significantly increased in the splenic MZ of wild-type mice but not in that of CatS-/- mice after PgLPS exposure. Furthermore, the increase in the Th17+ CD4+ T cell population paralleled increases in the levels of CatS and IL-6 in CD11c+ cells in the splenic MZ. In isolated primary splenic CD11c+ cells, the mRNA expression and the production of IL-6 was dramatically increased in wild-type mice but not in CatS-/- mice after direct stimulation with PgLPS (1 μg/ml, and this PgLPS-induced increase in the IL-6 expression was completely abolished by pre-treatment with Z-Phe-Leu-COCHO (Z-FL, the specific inhibitor of CatS. The PgLPS activated protease-activated receptor (PAR 2 in the isolated splenic CD11c+ cells was also significantly inhibited by CatS deficiently. In addition, the PgLPS-induced increase in the IL-6 production by splenic CD11c+ cells was completely abolished by pre-treatment with

  1. Installation of a bitumen coating plant for high-activity concentrates; Installation pilote d'enrobage par le bitume de concentrats de haute activite

    Energy Technology Data Exchange (ETDEWEB)

    Rodier, J; Lefillatre, G; Seyfried, P [Commissariat a l' Energie Atomique, Chusclan (France). Centre de Production de Plutonium de Marcoule

    1969-07-01

    Following the excellent results obtained on the industrial coating of radioactive sludges, the possibility of solidifying also the evaporation concentrates with bitumen has been considered. For high activity concentrates, the use of bitumen is however limited by two main parameters: temperature resistance, irradiation resistance. By making use of the characteristics of a blown bitumen, it has been possible to design a high activity coating pilot plant treating concentrations of several tens of curies per litre. This plant will make use of a screw-type extrusion machine capable of coating treated concentrates at a rate of 20 l/hr. Before being coated, the concentrates will be subjected to a coprecipitation treatment designed to make the radioelements insoluble. This installation will make possible, apart from technological studies, laboratory experiments on the coated material (measurements on self-heating, on electrical charges, on radiolytic gases, and also lixiviation tests. It is at present believed, on the basis of available data, that it is possible to coat concentrates having an activity of 20 Ci/l at a price of 1840 F per cubic metre. (authors) [French] A la suite des excellents resultats obtenus pour l'enrobage industriel des boues radioactives, il a ete envisage de solidifier egalement les concentrats d'evaporation par le bitume. Pour les concentrats de haute activite, l'emploi du bitume est cependant limite par deux parametres essentiels: tenue a la temperature, tenue a l'irradiation. Grace aux caracteristiques d'un bitume souffle, il a ete possible de concevoir une installation pilote d'enrobage haute activite contenant plusieurs dizaines de curies par litre. Cette installation utilisera une machine du type extrudeuse a vis qui peut enrober 20 l/h de concentrats traites. Avant leur enrobage, les concentrats subiront un traitement de coprecipitation destine a insolubiliser les radioelements. En dehors des etudes technologiques, cette installation

  2. Utilisation des courbes de débit fractionnaire pour l'analyse des phénomènes de récupération par tensio-actif Using Fractional Flow Curves to Analyze Surfactant Recovery Phenomena

    Directory of Open Access Journals (Sweden)

    Moulu J. C.

    2006-11-01

    Full Text Available La connaissance des relations débit fractionnaire-saturation obtenues à partir des courbes de perméabilité relative a permis : - d'expliquer les mécanismes de formation et de propagation des différentes phases mobiles (eau et huile en place, phase aqueuse contenant du tensio-actif et/ou de polymère ; - de prévoir l'allure des courbes de récupération (en particulier la composition et le moment d'arrivée du banc d'huile en déplacement secondaire ou tertiaire ; ces courbe concordent sensiblement avec celles que l'on obtient à partir d'expériences de récupération ; - de tenir compte du volume du bouchon du tensio-actif injecté ; - les paramètres des solutions injectées (tension interfaciale, viscosité, adsorption. . . ayant un effet sur la forme des profils de débit fractionnaire, de mettre en relief le points importants pour l'optimisation des déplacements. Ce type d'analyse par lequel on traite ici un nombre limité de cas peut être généralisé et servir à contrôler les résultats d'expériences ou de simulations numériques. Knowing the fractional flow-saturation relations obtained from relative-permeability curves has made it possible: - to explain the formation and propagation mechanisms of different mobile phases (water and oil in place, aqueous phase containing surfactant and/or polymer ; - to predict the shape of recovery curves (in particular the composition and arrival time of the oil bank for secondary or tertiary recovery; these curves more or less agree with the ones obtained from recovery experiments; - to take into consideration the volume of the surfactant slug injected; - to highlight the important points for optimizing displacements using the parameters of injected solutions (interfacial tension, viscosity, adsorption, etc. having an effect on the shape of fractional flow profiles. This type of analysis, in which a limited number of cases are dealt with here, can be generalized and used to check the

  3. Plasma suPAR is lowered by smoking cessation

    DEFF Research Database (Denmark)

    Eugen-Olsen, Jesper; Ladelund, Steen; Sørensen, Lars Tue

    2016-01-01

    BACKGROUND: Soluble urokinase plasminogen activator receptor (suPAR) is a stable inflammatory biomarker. In patients, suPAR is a marker of disease presence, severity and prognosis. In the general population, suPAR is predictive of disease development, such as diabetes and cardiovascular disease a...

  4. Copenhagen uPAR prostate cancer (CuPCa) database

    DEFF Research Database (Denmark)

    Lippert, Solvej; Berg, Kasper D; Høyer-Hansen, Gunilla

    2016-01-01

    AIM: Urokinase plasminogen activator receptor (uPAR) plays a central role during cancer invasion by facilitating pericellular proteolysis. We initiated the prospective 'Copenhagen uPAR Prostate Cancer' study to investigate the significance of uPAR levels in prostate cancer (PCa) patients. METHODS...

  5. Radio Column Chromatographic Assay of H{sup 3}-Labelled Substances; Analyse par radiochromatographie en colonne, de substances marquees par le tritium; Radiokhromatograficheskoe ispytanie mechennykh tritiem veshchestv na kolonke; Analisis por radiocromatografia en columna de sustancias marcadas con {sup 3}H

    Energy Technology Data Exchange (ETDEWEB)

    Scharpenseel, H W; Menke, K H [Institute of Soil Science and Institute of Animal Husbandry, Bonn University, Bonn (Germany)

    1962-01-15

    between the analyser- and monitor-phototubes. In order to ensure a laminar flow and constantly mixed volume ratio, dose-pumps are lised for the supply of buffer on the column and of the 30-fold volume of toluene/ethanol. The counting efficiency for H{sup 3} averages 2.5-4% for'coils spreading over the whole diameter of the phototubes. 3. The aqeous column effluent is led through an anthracene-filled glass tube and the H{sup 3} counted with close to l{sup o}/o efficiency. (author) [French] Dans la majorite des cas, les recherches radiochromatographique s combinees portant sur des substances marquees par {sup 3}H font partie integrante des experiences biochimiques utilisant des composes trities. Avec un ensemble comprenant un compteur a balayage continu sans fenetre, le comptage du tritium par radiochromatographi e sur papier est d'un rendement de 0,5 a 1,5%, qui depend dans une large mesure de l'epaisseur du papier et de l'autoabsorption dans le compose marque. La radiochromatographie en phase gazeuse des composes trities ne presente pas davantage de difficultes serieuses. On utilise avec succes un ensemble de chromatographic en phase gazeuse comportant une chambre d'ionisation a courant et un electrometre a lame vibrante - concu par K. E. Wilzbach et P. Riesz et perfectionne par H. Dutton, L. Mason et L. Blair. Grace a l'emploi de ''Teflon'' et de caoutchouc de silicones pour les elements isolants de la chambre d'ionisation, cet ensemble peut fonctionner a des temperatures voisines de 300{sup o}C. Avec le tritium, la radiochromatographi e en colonne donne des resultats mediocres, si le liquide sortant de la colonne se repand en une couche mince et passe lentement sous un compteur a balayage continu sans fenetre ou un compteur a scintillations, alors que le meme procede a donne d'excellente resultats avec le carbone-14. Aussi est-il probable que l'on adoptera la spectrometrie a scintillations en phase liquide. Elle comporte essentiellement deux procedes differents, que les

  6. La Vie de Marillac : un essai hagiographique ? Analyse comparée des sources utilisées par Nicolas Lefèvre de Lezeau dans la Vie de Marillac et la Vie de Morvillier

    Directory of Open Access Journals (Sweden)

    Dora De Lima

    2012-01-01

    Full Text Available Considérer Nicolas Lefèvre de Lezeau comme le biographe de son grand oncle ou comme l’hagiographe de son ami Michel de Marillac revient à glorifier la mémoire des trois héros au détriment du projet d’écriture de l’auteur. La Vie de Jean de Morvillier et la Vie de Michel de Marillac sont vivement marquées par l’omniprésence de leur auteur, quand bien même l’illusion de la richesse documentaire sur les trois héros voudrait l’estomper. Parce qu’ils convoquent une documentation riche et de nombreuses références littéraires, les trois récits de vie retrouvés témoignent d’une quête d’authenticité par leur auteur. En ce sens, la Vie de Marillac se dégage des deux autres récits de vie écrits par Lezeau. Le conseiller d’état n’y cherche effectivement pas seulement à raviver la mémoire de la Ligue dans le milieu dévot, ni même à construire une filiation entre ces deux partis. L’originalité de la Vie de Marillac tient de l’abondance des références spirituelles et de l’autorité que les mystiques incarnent pour sa validation. Pour les mystiques, la vie chargée d’un officier d’état demeure incompatible avec le mode de vie humble et contemplatif d’un « petit ». Du côté des politiques, la « cabale des dévots » représente une menace croissante pour l’autorité du roi. Dès lors, la Vie de Marillac de Lezeau nous invite à réfléchir sur les caractéristiques de rejet d’un modèle de sainteté (E. Suire. La postérité retient Marillac pour sa témérité et sa faillite face à la raison d’état de Richelieu, comme l’attestent le peu de biographies des xixe-xxe siècles de ce garde des sceaux. Parallèlement, si Marillac est décrit comme un laïc ayant fait preuve d’une grande dévotion dans les Histoires de l’ordre des Carmélites déchaussées parues aux xixe et xxe siècles, sa part active à la fondation de l’ordre en France ne suffit pas à faire de lui un saint

  7. Problems raised by pulse analysers - functional multi-dimensional analysers; Problemes poses par les analyseurs en impulsions - Analyseurs multidimensionnels fonctionnels; Problemy, voznikayushchie v svyazi s ispol'zovaniem analizatorov impul'sov - funktsional'nye mnogorazmernye analizatory; Problemas planteados por los analizadores de impulsos - Analizadores multidimensionales funcionales

    Energy Technology Data Exchange (ETDEWEB)

    Pages, A [Centre d' Etudes Nucleaires de Saclay (France)

    1962-04-15

    de nombreuses raisons: le nombre de parametres possibles est beaucoup trop reduit (deblocage seulement sur les analyseurs 100 et 256 canaux) ainsi que leur capacite; cette limitation multiplie considerablement le temps d'accelerateur; parfois l'experience est rendue impossible par le mauvais de ces analyseurs; par ailleurs, etant concus en un seul , ils manquent de d'utilisation. Un appareil multidimensionnel limite a 2 voies de 63 canaux a ete etudie et construit en 1958-59. Une separation est faite entre les differentes fonctions essentielles: analyse, transfert (fonctionnement en multidimensionnel) et exploitation des resultats. MM. Amram, Guillon et Thenard etudient et construisent a la SEG un analyseur multidimensionnel 1023 canaux x 63 canaux (destine au groupe de mesures de vitesses de neutrons de la SPNBE). Par ailleurs, afin de repondre aux limitations precedemment formulees, le laboratoire d'electronique de la SPNBE realise un analyseur multidimensionnel fonctionnel, ou la fonction de transfert a ete elle-meme decomposee en unites (rythmeur, memoire non integratrice, conditionneur et calculateur). Les types d'unites choisies sont, comme les limites definissant les voies, variables en fonction des besoins et de la complexite de l'experience (langage standard interfonctionnel). (author) [Spanish] Los investigadores emplean cada vez con mayor frecuencia conjuntos analizadores que permiten obtener una funcion de varios parametros (el numero de parametros y la precision relativa de cada uno de ellos puede variar de un experimento a otro). Por muchas razones, los analizadores actuales no se prestan bien para los experimentos, que son cada dia mas complejos: el numero de parametros posibles es excesivamente pequeno (desbloqueo solo en los analizadores de 100 y 256 canales) y tambien lo es su capacidad; esta limitacion multiplica considerablemente el tiempo de acelerador. Algunas veces, el bajo ''rendimiento'' de estos analizadores

  8. LOFT experimental measurements uncertainty analyses. Volume XX. Fluid-velocity measurement using pulsed-neutron activation

    International Nuclear Information System (INIS)

    Lassahn, G.D.; Taylor, D.J.N.

    1982-08-01

    Analyses of uncertainty components inherent in pulsed-neutron-activation (PNA) measurements in general and the Loss-of-Fluid-Test (LOFT) system in particular are given. Due to the LOFT system's unique conditions, previously-used techniques were modified to make the volocity measurement. These methods render a useful, cost-effective measurement with an estimated uncertainty of 11% of reading

  9. Meta-analyses of workplace physical activity and dietary behaviour interventions on weight outcomes

    NARCIS (Netherlands)

    Verweij, L.M.; Coffeng, J.; Mechelen, W. van; Proper, K.I.

    2011-01-01

    This meta-analytic review critically examines the effectiveness of workplace interventions targeting physical activity, dietary behaviour or both on weight outcomes. Data could be extracted from 22 studies published between 1980 and November 2009 for meta-analyses. The GRADE approach was used to

  10. Par Pond water balance

    International Nuclear Information System (INIS)

    Hiergesell, R.A.; Dixon, K.L.

    1996-06-01

    A water budget for the Par Pond hydrologic system was established in order to estimate the rate of groundwater influx to Par Pond. This estimate will be used in modeling exercises to predict Par Pond reservoir elevation and spillway discharge in the scenario where Savannah River water is no longer pumped and discharged into Par Pond. The principal of conservation of mass was used to develop the water budget, where water inflow was set equal to water outflow. Components of the water budget were identified, and the flux associated with each was determined. The water budget was considered balanced when inflow and outflow summed to zero. The results of this study suggest that Par Pond gains water from the groundwater system in the upper reaches of the reservoir, but looses water to the groundwater system near the dam. The rate of flux of groundwater from the water table aquifer into Par Pond was determined to be 13 cfs. The rate of flux from Par Pond to the water table aquifer near the dam was determined to be 7 cfs

  11. Regulatory support activities of JNES by thermal-hydraulic and safety analyses

    International Nuclear Information System (INIS)

    Kasahara, Fumio

    2008-01-01

    Current status and some related topics on regulatory support activities of Japan Nuclear Energy Safety Organization (JNES) by thermal-hydraulic and safety analyses are reported. The safety of nuclear facilities is secured primarily by plant owners and operators. However, the regulatory body NISA (Nuclear and Industrial Safety Agency) has conducted a strict regulation to confirm the adequacy of the site condition as well as the basic and detailed design. The JNES has been conducting independent analyses from applicants (audit analyses, etc.) by direction of NISA and supporting its review. In addition to the audit analysis, thermal-hydraulic and safety analyses are used in such areas as analytical evaluation for investigation of causes of accidents and troubles, level 2 PSA for risk informed regulation, etc. Recent activities of audit analyses are for the application of Tsuruga 3 and 4 (APWR), the spent fuel storage facility for the establishment, and the LMFBR Monju for the core change. For the trouble event evaluation, the criticality accident analysis of Sika1 was carried out and the evaluation of effectiveness of accident management (AM) measure for Tomari 3 (PWR) and Monju was performed. The analytical codes for these evaluations are continuously revised by reflecting the state-of-art technical information and validated using the information provided by the data from JAEA, OECD project, etc. (author)

  12. FooPar

    DEFF Research Database (Denmark)

    Hargreaves, F. P.; Merkle, D.

    2013-01-01

    We present FooPar, an extension for highly efficient Parallel Computing in the multi-paradigm programming language Scala. Scala offers concise and clean syntax and integrates functional programming features. Our framework FooPar combines these features with parallel computing techniques. Foo......, results based on a empirical analysis on two supercomputers are given. We achieve close-to-optimal performance wrt. theoretical peak performance. Based on this result we conclude that FooPar allows programmers to fully access Scalas design features without suffering from performance drops when compared...

  13. Stuttering, induced fluency, and natural fluency: a hierarchical series of activation likelihood estimation meta-analyses.

    Science.gov (United States)

    Budde, Kristin S; Barron, Daniel S; Fox, Peter T

    2014-12-01

    Developmental stuttering is a speech disorder most likely due to a heritable form of developmental dysmyelination impairing the function of the speech-motor system. Speech-induced brain-activation patterns in persons who stutter (PWS) are anomalous in various ways; the consistency of these aberrant patterns is a matter of ongoing debate. Here, we present a hierarchical series of coordinate-based meta-analyses addressing this issue. Two tiers of meta-analyses were performed on a 17-paper dataset (202 PWS; 167 fluent controls). Four large-scale (top-tier) meta-analyses were performed, two for each subject group (PWS and controls). These analyses robustly confirmed the regional effects previously postulated as "neural signatures of stuttering" (Brown, Ingham, Ingham, Laird, & Fox, 2005) and extended this designation to additional regions. Two smaller-scale (lower-tier) meta-analyses refined the interpretation of the large-scale analyses: (1) a between-group contrast targeting differences between PWS and controls (stuttering trait); and (2) a within-group contrast (PWS only) of stuttering with induced fluency (stuttering state). Copyright © 2014 Elsevier Inc. All rights reserved.

  14. Study of elementary mechanisms of creep in uranium as a function of temperature (150 deg. to 760 deg. C) by activation energy measurements; Etude des mecanismes elementaires de deformation par fluage de l'uranium en fonction de la temperature (de 150 deg. a 760 deg. C) par la mesure des energies d'activation

    Energy Technology Data Exchange (ETDEWEB)

    Grenier, P [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

    1967-07-01

    determination de l'energie d'activation pour le fluage et l'etude de ses variations en fonction de la temperature ont permis de definir differents domaines de temperature pour lesquels un ou plusieurs mecanismes elementaires controlent la deformation. Les observations micrographiques effectuees directement apres fluage et l'etude des variations de la vitesse de fluage en fonction de la charge appliquee confirment les hypotheses prononcees. Au-dessous de 325 deg. C, le comportement au fluage des monocristaux et des polycristaux est identique. De 325 deg. C a une limite superieure de temperature dependant de l'etat structural du metal et de son degre de purete, la deformation par fluage de l'uranium est controlee par un glissement devie. De ces limites de temperature a 520 deg. C, le fluage de l'uranium fait intervenir deux mecanismes simultanes et independants, le deplacement des dislocations-vis par un systeme de glissement devie et la montee d'une partie des dislocations-coins hors de leur plan de glissement. Au-dessus de 520 deg. C jusqu'au haut de la phase {alpha}, le fluage des polycristaux est controle par la montee des dislocations-coins hors de leur plan de glissement, suivant un mecanisme d'empilement dans le cas du fluage primaire et d'annihilation de dipoles dans le cas du fluage secondaire. Pour les monocristaux, le fluage depend de la montee des dislocations-coins dans les sous-joints preexistant au fluage et de leur rearrangement ulterieur au sein de ces sous-joints. En phase {beta}, le fluage des polycristaux est controle par la montee des dislocations-coins par diffusion. Entre 450 et 630 deg. C, l'addition de faibles teneurs de molybdene a l'uranium modifie les caracteristiques du fluage, tout en conservant des mecanismes de deformation analogue a ceux de l'uranium non allie. (auteur)

  15. Endothelial microparticles released by activated protein C protect beta cells through EPCR/PAR1 and annexin A1/FPR2 pathways in islets.

    Science.gov (United States)

    Kreutter, Guillaume; Kassem, Mohamad; El Habhab, Ali; Baltzinger, Philippe; Abbas, Malak; Boisrame-Helms, Julie; Amoura, Lamia; Peluso, Jean; Yver, Blandine; Fatiha, Zobairi; Ubeaud-Sequier, Geneviève; Kessler, Laurence; Toti, Florence

    2017-11-01

    Islet transplantation is associated with early ischaemia/reperfusion, localized coagulation and redox-sensitive endothelial dysfunction. In animal models, islet cytoprotection by activated protein C (aPC) restores islet vascularization and protects graft function, suggesting that aPC triggers various lineages. aPC also prompts the release of endothelial MP that bear EPCR, its specific receptor. Microparticles (MP) are plasma membrane procoagulant vesicles, surrogate markers of stress and cellular effectors. We measured the cytoprotective effects of aPC on endothelial and insulin-secreting Rin-m5f β-cells and its role in autocrine and paracrine MP-mediated cell crosstalk under conditions of oxidative stress. MP from aPC-treated primary endothelial (EC) or β-cells were applied to H 2 O 2 -treated Rin-m5f. aPC activity was measured by enzymatic assay and ROS species by dihydroethidium. The capture of PKH26-stained MP and the expression of EPCR were probed by fluorescence microscopy and apoptosis by flow cytometry. aPC treatment enhanced both annexin A1 (ANXA1) and PAR-1 expression in EC and to a lesser extent in β-cells. MP from aPC-treated EC (eM aPC ) exhibited high EPCR and annexin A1 content, protected β-cells, restored insulin secretion and were captured by 80% of β cells in a phosphatidylserine and ANXA1-dependent mechanism. eMP activated EPCR/PAR-1 and ANXA1/FPR2-dependent pathways and up-regulated the expression of EPCR, and of FPR2/ALX, the ANXA1 receptor. Cytoprotection was confirmed in H 2 O 2 -treated rat islets with increased viability (62% versus 48% H 2 O 2 ), reduced apoptosis and preserved insulin secretion in response to glucose elevation (16 versus 5 ng/ml insulin per 10 islets). MP may prove a promising therapeutic tool in the protection of transplanted islets. © 2017 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.

  16. Prognostic value of plasma soluble urokinase plasminogen activator receptor (suPAR) in Danish patients with recurrent epithelial ovarian cancer (REOC)

    DEFF Research Database (Denmark)

    Begum, Farah Diba; Høgdall, Estrid V S; Riisbo, Rikke

    2006-01-01

    ovarian cancer (REOC). For determination of suPAR, pre-chemotherapeutic blood samples from the patients with REOC were processed into plasma (EDTA) within one working day from venipuncture. The plasma suPAR level is not correlated with performance status (p=0.41), FIGO stage (p=0.09), treatment...... of REOC patients. Multivariate analysis showed that only TFI of 12 months (p=0.001) and performance score status of 2 (p=0.02) were independent prognostic factors. Our study indicates that pre-chemotherapeutic measurement of plasma suPAR level in REOC patients may not be useful to identify a subgroup...... of patients with poor prognosis....

  17. Semisynthetic Lipopeptides Derived from Nisin Display Antibacterial Activity and Lipid II Binding on Par with That of the Parent Compound

    NARCIS (Netherlands)

    Koopmans, Timo; Wood, Thomas M.; 't Hart, Peter; Kleijn, Laurens H. J.; Hendrickx, Antoni P. A.; Willems, Rob J. L.; Breukink, Eefjan; Martin, Nathaniel I.

    2015-01-01

    The lipid II-binding N-terminus of nisin, comprising the so-called A/B ring system, was synthetically modified to provide antibacterially active and proteolytically stable derivatives. A variety of lipids were coupled to the C-terminus of the nisin A/B ring system to generate semisynthetic

  18. Initial testing of a neutron activation analysis system by analysing standard reference materials

    International Nuclear Information System (INIS)

    Suhaimi Hamzah; Roslan Idris; Abdul Khalik Haji Wood; Che Seman Mahmood; Abdul Rahim Mohamad Noor.

    1983-01-01

    This paper describes the data acquisition and processing system in our laboratories (ND6600), the methods of activation analysis and the results obtained from our analysis of IAEA standard reference material (SL-l lake sediments and NBS coal ash 1632a). These standards were analysed in order to check the capability of the system, which was designed in such a way as to enable the user to independently collect and process data from multiple radiation detectors. (author)

  19. A Bayesian approach to assess data from radionuclide activity analyses in environmental samples

    International Nuclear Information System (INIS)

    Barrera, Manuel; Lourdes Romero, M.; Nunez-Lagos, Rafael; Bernardo, Jose M.

    2007-01-01

    A Bayesian statistical approach is introduced to assess experimental data from the analyses of radionuclide activity concentration in environmental samples (low activities). A theoretical model has been developed that allows the use of known prior information about the value of the measurand (activity), together with the experimental value determined through the measurement. The model has been applied to data of the Inter-laboratory Proficiency Test organised periodically among Spanish environmental radioactivity laboratories that are producing the radiochemical results for the Spanish radioactive monitoring network. A global improvement of laboratories performance is produced when this prior information is taken into account. The prior information used in this methodology is an interval within which the activity is known to be contained, but it could be extended to any other experimental quantity with a different type of prior information available

  20. Serum suPAR in patients with FSGS: trash or treasure?

    NARCIS (Netherlands)

    Maas, R.J.H.; Deegens, J.K.J.; Wetzels, J.F.M.

    2013-01-01

    The urokinase-type plasminogen activator receptor (uPAR) has important functions in cell migration. uPAR can be shed from the cell membrane resulting in soluble uPAR (suPAR). Further cleavage gives rise to shorter fragments with largely unknown functions. Recent studies have demonstrated that both

  1. Structural and functional analyses of DNA-sensing and immune activation by human cGAS.

    Science.gov (United States)

    Kato, Kazuki; Ishii, Ryohei; Goto, Eiji; Ishitani, Ryuichiro; Tokunaga, Fuminori; Nureki, Osamu

    2013-01-01

    The detection of cytosolic DNA, derived from pathogens or host cells, by cytosolic receptors is essential for appropriate host immune responses. Cyclic GMP-AMP synthase (cGAS) is a newly identified cytosolic DNA receptor that produces cyclic GMP-AMP, which activates stimulator of interferon genes (STING), resulting in TBK1-IRF3 pathway activation followed by the production of type I interferons. Here we report the crystal structure of human cGAS. The structure revealed that a cluster of lysine and arginine residues forms the positively charged DNA binding surface of human cGAS, which is important for the STING-dependent immune activation. A structural comparison with other previously determined cGASs and our functional analyses suggested that a conserved zinc finger motif and a leucine residue on the DNA binding surface are crucial for the DNA-specific immune response of human cGAS, consistent with previous work. These structural features properly orient the DNA binding to cGAS, which is critical for DNA-induced cGAS activation and STING-dependent immune activation. Furthermore, we showed that the cGAS-induced activation of STING also involves the activation of the NF-κB and IRF3 pathways. Our results indicated that cGAS is a DNA sensor that efficiently activates the host immune system by inducing two distinct pathways.

  2. Structural and functional analyses of DNA-sensing and immune activation by human cGAS.

    Directory of Open Access Journals (Sweden)

    Kazuki Kato

    Full Text Available The detection of cytosolic DNA, derived from pathogens or host cells, by cytosolic receptors is essential for appropriate host immune responses. Cyclic GMP-AMP synthase (cGAS is a newly identified cytosolic DNA receptor that produces cyclic GMP-AMP, which activates stimulator of interferon genes (STING, resulting in TBK1-IRF3 pathway activation followed by the production of type I interferons. Here we report the crystal structure of human cGAS. The structure revealed that a cluster of lysine and arginine residues forms the positively charged DNA binding surface of human cGAS, which is important for the STING-dependent immune activation. A structural comparison with other previously determined cGASs and our functional analyses suggested that a conserved zinc finger motif and a leucine residue on the DNA binding surface are crucial for the DNA-specific immune response of human cGAS, consistent with previous work. These structural features properly orient the DNA binding to cGAS, which is critical for DNA-induced cGAS activation and STING-dependent immune activation. Furthermore, we showed that the cGAS-induced activation of STING also involves the activation of the NF-κB and IRF3 pathways. Our results indicated that cGAS is a DNA sensor that efficiently activates the host immune system by inducing two distinct pathways.

  3. Performance Analysis of Compositional and Modified Black-Oil Models For a Gas Lift Process Analyse des performances de modèles black-oil pour le procédé d’extraction par injection de gaz

    Directory of Open Access Journals (Sweden)

    Mahmudi M.

    2013-03-01

    Full Text Available Artificial gas lift is frequently used to boost production rate of mature oil fields. An integrated mathematical model was developed in order to track the spatial and temporal changes in various components of the continuous gas lift process. The computational demand for solving the comprehensive gas lift model highly depends on the thermodynamic treatment of the hydrocarbon fluids involved. A full compositional treatment using an equation of state provides the most accurate results but at a high computational cost. The results of this article showed that the computational cost can be halved without sacrificing accuracy by using reduced parameter stability and flash calculation procedures. It was also demonstrated that a Modified Black-Oil treatment of the fluids can provide reasonable accuracy at a much-reduced computational cost. The Modified Black-Oil treatment provides a valuable tool when the model has to be solved many hundreds of times to find the optimal combination of the gas lift parameters. Les procédés artificiels d’extraction par injection de gaz sont utilisés pour améliorer le taux de récupération des champs pétroliers matures. Un modèle mathématique intégré a été développé pour détecter de faibles changements temporels et spatiaux dans plusieurs composants des procédés continus d’extraction par injection de gaz. La solution numérique utilisée pour résoudre le modèle du procédé d’extraction dépend fortement du comportement thermodynamique des hydrocarbures impliqués. Un traitement complet de la composition utilisant une équation d’état offre les résultats les plus précis, mais à un coût de calcul très élevé. Les résultats de nos travaux de recherche montrent que l’implication des paramètres de stabilité et des procédures de calcul flash, peut diviser par deux le coût du calcul tout en gardant la précision attendue. Ces travaux montrent que la précision admissible peut être

  4. uPAR Expression Pattern in Patients with Urothelial Carcinoma of the Bladder--Possible Clinical Implications.

    Directory of Open Access Journals (Sweden)

    Line Hammer Dohn

    Full Text Available The objective of the present study was to confirm the expression and localisation pattern of the urokinase-type plasminogen activator receptor (uPAR focusing on its possible clinical relevance in patients with urothelial neoplasia of the bladder. uPAR is a central molecule in tissue remodelling during cancer invasion and metastasis and is an established prognostic marker in various cancer diseases other than bladder cancer. Formalin-fixed and paraffin-embedded tumour-tissue blocks from 186 patients treated with radical cystectomy were analysed. uPAR expression was scored as either negative or positive as well as by the actual score. Separate scores were obtained for cancer cells, macrophages and myofibroblasts at the invasive front and in tumour core. We were able to confirm, in an independent patient cohort, the tissue expression and localisation pattern of uPAR as investigated by Immunohistochemistry as well as a significant association between uPAR positivity and increasing tumour stage and tumour grade. This demonstrates the robustness of our previous and current findings. In addition the association between uPAR positive myofibroblasts and poor survival was reproduced. The highest hazard ratios for survival were seen for uPAR positive myofibroblasts both at the invasive front and in tumour core. Evaluating uPAR expression by the actual score showed a significant association between uPAR positive myofibroblasts in tumour core and an increased risk of cancer specific mortality. Our investigations have generated new and valuable biological information about the cell types being involved in tumour invasion and progression through the plasminogen activation system.

  5. The relationship between hope and patient activation in consumers with schizophrenia: Results from longitudinal analyses.

    Science.gov (United States)

    Oles, Sylwia K; Fukui, Sadaaki; Rand, Kevin L; Salyers, Michelle P

    2015-08-30

    Hope (goal-directed thinking) and patient activation (knowledge and skills to manage one's illness) are both important in managing chronic conditions like schizophrenia. The relationship between hope and patient activation has not been clearly defined. However, hope may be viewed as a foundational, motivating factor that can lead to greater involvement in care and feelings of efficacy. The purpose of the present study was to understand the prospective relationship between hope and patient activation in a sample of adults with schizophrenia (N=118). This study was a secondary data analysis from a study on Illness Management and Recovery (IMR) - a curriculum-based approach to schizophrenia self-management. Data were collected at baseline (prior to any intervention), and at 9 and 18-month follow-up. As predicted, hope and patient activation were significantly related with each other, showing large positive concurrent correlations. Demographics and background characteristics were not significantly related to patient activation or hope. Longitudinal analyses found no specific directional effect, yet suggested that hope and patient activation mutually influence each other over time. Our findings add flexibility in designing recovery-based interventions - fostering hope may not be a pre-requisite for activating consumers to be more involved in their own care. Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

  6. Meta-analyses of workplace physical activity and dietary behaviour interventions on weight outcomes.

    Science.gov (United States)

    Verweij, L M; Coffeng, J; van Mechelen, W; Proper, K I

    2011-06-01

    This meta-analytic review critically examines the effectiveness of workplace interventions targeting physical activity, dietary behaviour or both on weight outcomes. Data could be extracted from 22 studies published between 1980 and November 2009 for meta-analyses. The GRADE approach was used to determine the level of evidence for each pooled outcome measure. Results show moderate quality of evidence that workplace physical activity and dietary behaviour interventions significantly reduce body weight (nine studies; mean difference [MD]-1.19 kg [95% CI -1.64 to -0.74]), body mass index (BMI) (11 studies; MD -0.34 kg m⁻² [95% CI -0.46 to -0.22]) and body fat percentage calculated from sum of skin-folds (three studies; MD -1.12% [95% CI -1.86 to -0.38]). There is low quality of evidence that workplace physical activity interventions significantly reduce body weight and BMI. Effects on percentage body fat calculated from bioelectrical impedance or hydrostatic weighing, waist circumference, sum of skin-folds and waist-hip ratio could not be investigated properly because of a lack of studies. Subgroup analyses showed a greater reduction in body weight of physical activity and diet interventions containing an environmental component. As the clinical relevance of the pooled effects may be substantial on a population level, we recommend workplace physical activity and dietary behaviour interventions, including an environment component, in order to prevent weight gain. © 2010 The Authors. obesity reviews © 2010 International Association for the Study of Obesity.

  7. Detectable elements in a particles pattern of suspended urban matter analysed by neutron activation

    International Nuclear Information System (INIS)

    Herrera, L.; Beltran, C.; Alemon, E.; Ortiz, M.E.

    2001-01-01

    The multielement composition of a Standard Reference Material 1648 pattern certified is reported and it is used for the suspended in air aerosol samples analysis from urban localities of the Valley of Mexico, which was irradiated in the same geometry of the sample. The bottom of laboratory is analysed where was made the gamma spectrometry and it is compared the ratio of country up of bottom photo peaks with pattern photo peaks in nearer interest regions. The bottom natural gamma transmitters were identified and those of the activated pattern in the TRIGA Mark III nuclear reactor. (Author)

  8. It's quicker "Par Avignon"!

    CERN Multimedia

    2005-01-01

    For a few years, the CERN Library has been receiving books from the University of Hanover sent via Avignon, at least that's what it says on the envelope. Such a detour would mean that parcels were travelling 720 km more than the distance separating Geneva and Hanover, which would be a very strange state of affairs. The explanation lies in a spelling mistake. The sender has been stamping parcels with a stamp that says "Par Avignon prioritaire" (first-class via Avignon) instead of "Par Avion prioritaire" (First Class Air Mail), a source of much amusement to the librarians!

  9. Activation and afterheat analyses for the HCPB test blanket module in ITER

    International Nuclear Information System (INIS)

    Pereslavtsev, P.; Fischer, U.

    2008-01-01

    To provide a sound data basis for the safety analyses of the HCPB TBM system in ITER, the afterheat and activity inventories were assessed making use of a code system that allows performing 3D activation calculations by linking the Monte Carlo transport code MCNP and the fusion inventory code FISPACT through an appropriate interface. A suitable MCNP model of a 20 deg. ITER torus sector with an integrated TBM of the HCPB PI (plant integration) type in the horizontal test blanket port was developed and adapted to the requirements for coupled 3D neutron transport and activation calculations. Two different irradiation scenarios were considered in the coupled 3D neutron transport and activation calculations. The first one is representative for the TBM irradiation in ITER with a total of 9000 neutron pulses over a 3 (calendar) years period. The second (conservative) irradiation scenario assumes an extended irradiation time over the full anticipated lifetime of ITER. The radioactivity inventories, the afterheat and the contact gamma dose were calculated as function of the decay time. Data were processed for the total activity, afterheat and contact dose rates of the TBM, its constituting components and materials

  10. Le souffre thiophénique dans les pétroles et les extraits de roche. Analyse par spectrométrie de masse et chromatographie en phase gazeuse Mass-Spectrometry and Gas-Chromatography Analysis of Thiophene Sulfur in Oil and Rock Extracts

    Directory of Open Access Journals (Sweden)

    Castex H.

    2006-11-01

    Full Text Available On étudie par spectrométrie de masse à haute résolution la répartition du soufre thiophénique par coupe de distillation et par nombre de carbone dans la fraction > C10 d'un pétrole brut. Parallèlement on chromatographie à l'aide d'un détecteur sélectif à photométrie de flamme les diverses fractions thiophéniques. L'examen des chromatogrammes permet de déterminer les zones dé réponse et l'allure générale des différentes coupes thiophéniques. Les applications sont variées : on vérifie le bien-fondé de l'utilisation d'un spectromètre de masse à basse résolution pour les analyses en routine d'huiles brutes et on peut, par la connaissance des différents profils de type thiophénique prévoir des analogies ou des différences au sein d'huiles brutes et d'extraits de roche d'un même bassin pétrolier, ce qui limite le nombre d'échantillons à analyser en détail. High-resolution mass spectrometry is used to analyze the distribution of thiophenic sulfur per distillation cut and per carbon number in the > C10 fraction of a crude oil. At the same time, a selective flame-photometry sensor is used to chromatograph the different thiophenic fractions. An analysis of the chromatograms serves to determine the response zones and the general aspect of the different thiophenic cuts. There are various applications of this process. The validity of using a low-resolution mass spectrometer is checked for routine crude-cil analyses. By determining the different profiles of the thiophenic type, similarities or differences can be predicted in the crude cils and rock extracts from the saure cil basin. This limits the number of samples having to be analyzed in detail.

  11. Determination of the ruthenium, cerium and zirconium radio-activity of sea-water by carrying-over and adsorption using manganese dioxide; Determination de la radioactivite de l'eau de mer en ruthenium, cerium, zirconium par en- trainement et adsorption au moyen du bioxyde de manganese

    Energy Technology Data Exchange (ETDEWEB)

    Guegueniat, P [Commissariat a l' Energie Atomique, La Hague (France). Centre d' Etudes Nucleaires

    1967-07-01

    Principle: Manganese dioxide is precipitated in the medium to be analyzed by the action of hydrogen peroxide on potassium permanganate. Large volumes of sea-water are treated by successive adsorptions of 80 litre fractions using always the same precipitate obtained from 30 g of potassium permanganate. Some examples are given concerning the analysis of 80, 160, 1000 and 2000 litres of water. Advantages of the technique: The existence of low activities due to ruthenium, zirconium and cerium can be demonstrated if sufficiently large volumes of water are treated. (author) [French] Principe: Le bioxyde de manganese est precipite dans le milieu a analyser par action de l'eau oxygenee sur le permanganate de potassium. Le traitement de grands volumes d'eau de mer se fait par adsorptions successives de fractions de 80 litres en utilisant toujours le meme precipite obtenu a partir de 30 g de permanganate de potassium. Quelques exemples ayant trait a des analyses de 80, 160, 1000, 2000 litres sont donnes. Interet de la technique: De faibles activites dues au Ruthenium, Zirconium, Cerium peuvent etre mises en evidence en traitant des volumes d'eau suffisants. (auteur)

  12. Regulation of Substantia Nigra Pars Reticulata GABAergic Neuron Activity by H2O2 via Flufenamic Acid-Sensitive Channels and KATP Channels

    Science.gov (United States)

    Lee, Christian R.; Witkovsky, Paul; Rice, Margaret E.

    2011-01-01

    Substantia nigra pars reticulata (SNr) GABAergic neurons are key output neurons of the basal ganglia. Given the role of these neurons in motor control, it is important to understand factors that regulate their firing rate and pattern. One potential regulator is hydrogen peroxide (H2O2), a reactive oxygen species that is increasingly recognized as a neuromodulator. We used whole-cell current clamp recordings of SNr GABAergic neurons in guinea-pig midbrain slices to determine how H2O2 affects the activity of these neurons and to explore the classes of ion channels underlying those effects. Elevation of H2O2 levels caused an increase in the spontaneous firing rate of SNr GABAergic neurons, whether by application of exogenous H2O2 or amplification of endogenous H2O2 through inhibition of glutathione peroxidase with mercaptosuccinate. This effect was reversed by flufenamic acid (FFA), implicating transient receptor potential (TRP) channels. Conversely, depletion of endogenous H2O2 by catalase, a peroxidase enzyme, decreased spontaneous firing rate and firing precision of SNr neurons, demonstrating tonic control of firing rate by H2O2. Elevation of H2O2 in the presence of FFA revealed an inhibition of tonic firing that was prevented by blockade of ATP-sensitive K+ (KATP) channels with glibenclamide. In contrast to guinea-pig SNr neurons, the dominant effect of H2O2 elevation in mouse SNr GABAergic neurons was hyperpolarization, indicating a species difference in H2O2-dependent regulation. Thus, H2O2 is an endogenous modulator of SNr GABAergic neurons, acting primarily through presumed TRP channels in guinea-pig SNr, with additional modulation via KATP channels to regulate SNr output. PMID:21503158

  13. Study of a micro-sublimation apparatus with removal of the vapours by pumping; application to the analysis of fluorinated products (1963); Etude d'un appareillage de microsublimation avec entrainement des vapeurs par pompage et application a l'analyse des produits fluores (1963)

    Energy Technology Data Exchange (ETDEWEB)

    Delvalle, P [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

    1963-07-01

    Micro-sublimation analysis presents definite advantages both from the qualitative and quantitative points of view. An automatic micro-sublimation analysis apparatus has been developed for the analysis of fluorinated products (ClF{sub 3}, HF, UF{sub 6}, etc.) but this is only one particular application of a method which has a far wider field of possible applications. We give first the most favorable conditions for the operation of such an apparatus. These conditions are the use of a detector which is linear and independent of the nature of the gas, the flow of the sublimed vapours in the conditions of molecular flow, and finally a reproducible and linear re-heating of the separating trap. The apparatus thus built has the advantage of yielding any analysis without prior calibration. It also makes possible the easy identification of an unknown product by the determination of its vapour pressure curve and its molecular weight. The analysis of fluorinated products with this apparatus has shown that the experimental results agree well with what is expected. (author) [French] L'analyse par microsublimation presente un interet certain tant au point de vue qualitatif que quantitatif. Nous avons mis au point un appareillage automatique d'analyse par microsublimation pour l'analyse des produits fluores (CIF{sub 3}, HF, UF{sub 6}, etc.) mais il ne s'agit que de l'application a un cas particulier d'une methode ayant un champ d'applications bien plus vaste. Nous exposons tout d'abord les conditions les plus favorables au bon fonctionnement d'un tel appareil. Ces conditions sont l'emploi d'un detecteur lineaire et independant de la nature du gaz, l'ecoulement des vapeurs sublimees en regime moleculaire et enfin un rechauffage reproductible et lineaire du piege separateur. L'appareil ainsi realise presente l'avantage d'effectuer une analyse quelconque sans etalonnage prealable. Il permet en outre d'identifier aisement un corps inconnu par la determination de sa courbe de tension

  14. Application of Fast Neutron Activity for Analysing Element Content on the Air Particulate

    International Nuclear Information System (INIS)

    Elin Nuraini; Ngasifudin; Sunardi; Elisabeth

    2003-01-01

    The research on application of fast neutron activation analysis for analysing element content on the air particulate has been done. The research about analysis of the particulate matters contained in non industrial traffic territory of Surakarta and full industrial traffic territory of Karanganyar, had been done using Fast Neutron Activation Analysis Method. Fast Neutron Activation Analysis method is one of the element analysis method which it's basic principle causes radioactivity appearance from the samples after being irradiated by neutron. The qualitative analysis method is based on the measuring of specific energy which was radiated by radioactive's nucleus and quantitative analysis method is based on the measuring of the intensity of each peak gamma energy. The qualitative analysis results showed, some element were identified i.e : 51 V ; 200 Pb, 27 Al and 52 Cr. The result showed that Pb level is 2.21 ± 0.09x10 -1 mg/m 3 in non industrial traffic territory of Surakarta and 2.78 ± 0.11x10 -1 mg/m 3 full industrial traffic territory of Karanganyar, this value greater than threshold value according 6.0x10 -2 mg/m 3 . (author)

  15. Activation analyses of authenticated hairs of Napoleon Bonaparte confirm arsenic poisoning.

    Science.gov (United States)

    Weider, B; Fournier, J H

    1999-12-01

    In 1960, activation analyses at the Harwell Nuclear Research Laboratory of the University of Glascow, London of authenticated hairs of Napoleon Bonaparte taken immediately after his death confirmed Napoleon's chronic arsenic poisoning on the island of St. Helena. Timeline correlation of his clinical symptomatology of the preceding 4 months, as reported in the written diaries of his exiled companions, further supports the effect of fluctuating, elevated toxic levels of arsenic on his health. Independent analyses of authenticated hairs of Napoleon by the Toxicology Crime Laboratory of the United States Federal Bureau of Investigation in 1995 reveals toxic levels of arsenic. The successful assassination of Napoleon included both a cosmetic and lethal phase. The cosmetic phase consisted of arsenic poisoning over time to weaken Napoleon, making the associated debility appear to be a natural illness and thus allay any suspicions prior to instituting the lethal phase. On May 3, 1821, at 5:30 P.M., the lethal phase was carried out. Napoleon was given Calomel (HgCl), a cathartic, and a popular orange-flavored drink called orgeat, which was flavored with the oil of bitter almonds. Together they formed mercury cyanide, which is lethal. Napoleon lost consciousness and died two days later.

  16. Urokinase-type plasminogen activator receptor (uPAR) on tumor-associated macrophages is a marker of poor prognosis in colorectal cancer

    DEFF Research Database (Denmark)

    Illemann, Martin; Laerum, Ole Didrik; Hasselby, Jane Preuss

    2014-01-01

    Patients were identified from a population-based prospective study of 4990 individuals with symptoms associated with colorectal cancer (CRC). A total of 244 CRC tissue samples were available for immunohistochemical staining of uPAR, semiquantitatively scored at the invasive front, and in the tumo...

  17. La pelade par plaques

    Science.gov (United States)

    Spano, Frank; Donovan, Jeff C.

    2015-01-01

    Résumé Objectif Présenter aux médecins de famille des renseignements de base pour faire comprendre l’épidémiologie, la pathogenèse, l’histologie et l’approche clinique au diagnostic de la pelade par plaques. Sources des données Une recension a été effectuée dans PubMed pour trouver des articles pertinents concernant la pathogenèse, le diagnostic et le pronostic de la pelade par plaques. Message principal La pelade par plaques est une forme de perte pileuse auto-immune dont la prévalence durant une vie est d’environ 2 %. Des antécédents personnels ou familiaux de troubles auto-immuns concomitants, comme le vitiligo ou une maladie de la thyroïde, peuvent être observés dans un petit sous-groupe de patients. Le diagnostic peut souvent être posé de manière clinique en se fondant sur la perte de cheveux non cicatricielle et circulaire caractéristique, accompagnée de cheveux en « point d’exclamation » en périphérie chez ceux dont le problème en est aux premiers stades. Le diagnostic des cas plus complexes ou des présentations inhabituelles peut être facilité par une biopsie et un examen histologique. Le pronostic varie largement et de mauvais résultats sont associés à une apparition à un âge précoce, une perte importante, la variante ophiasis, des changements aux ongles, des antécédents familiaux ou des troubles auto-immuns concomitants. Conclusion La pelade par plaques est une forme auto-immune de perte de cheveux périodiquement observée en soins primaires. Les médecins de famille sont bien placés pour identifier la pelade par plaques, déterminer la gravité de la maladie et poser le diagnostic différentiel approprié. De plus, ils sont en mesure de renseigner leurs patients à propos de l’évolution clinique de la maladie ainsi que du pronostic général selon le sous-type de patients.

  18. Assessment of the Total Body Burden of Caesium-137 in Humans by the Analysis of Blood; Determination de la Charge Corporelle de Cesium 137 chez l'Homme, par Analyse Du Sang; 041e 041f 0420 0415 0414 ; Evaluacion de la Carga Corporal Total de Cesio-137 en el Hombre por Analisis de Sangre

    Energy Technology Data Exchange (ETDEWEB)

    Yamagata, N. [Institute of Public Health, Tokyo (Japan)

    1964-11-15

    'anthropogammametres et ceux-ci sont des appareils fixes; dans ces conditions, il n'est pas facile de determiner la charge moyenne pour une population. Toutefois, la collecte de tissus musculaires ainsi preleves est une operation compliquee, en outre, cette methode n'est pas satisfaisante, car il faudrait en realite posseder des echantillons preleves immediatement apres la mort. Etant donne que l'on a seulement besoin de donnees susceptibles d'etre utilisees pour determiner la charge moyenne de cesium 137 pour une population donnee et qu'il n'est pas necessaire de connaitre les valeurs individuelles, il suffit d'avoir des echantillons globaux provenant du plus grand nombre possible d'individus. Aussi a-t-on pense que des echantillons de sang pur pourraient remplacer le mieux les tissus musculaires, parce qu'il existe deja un reseau de ramassage du sang, ramife dans tout le Japon (notamment 47 banques du sang autorisees et une centaine d'hopitaux). On procede deja, a l'Institut national de la sante, de Tokyo, a des enquetes par sondages pour verifier les caracteristiques du sang citrate. Environ 500 ampoules sont examinees chaque annee; cet echantillon represente plus de trois millions de donneurs repartis dans l'ensemble du pays. Pour cette verification, on n'utilise que la moitie du contenu des ampoules et on met en stock l'autre moitie pour qu'elle serve a preparer un echantillon global en vue de la determination de la charge moyenne de cesium 137, Le sang etant incinere, on dose le cesium stable contenu dans les cendres par une methode d'activation par les neutrons. Des resultats, on deduit le rapport entre la concentration de cette substance dans le sang pur et la quantite totale contenue dans le corps (1,4 mg pour un homme de 70 kg). Ce chiffre sera le meme que pour le cesium 137 dans des conditions d'equilibre. On a compare les resultats obtenus depuis juin 1963 par cette methode avec les resultats trouves par l'analyse de tissus musculaires pendant la meme periode. On a constate

  19. Iron interference in arsenic absorption by different plant species, analysed by neutron activation, k0-method

    International Nuclear Information System (INIS)

    Uemura, George; Matos, Ludmila Vieira da Silva; Silva, Maria Aparecida da; Menezes, Maria Angela de Barros Correia

    2009-01-01

    Natural arsenic contamination is a cause for concern in many countries of the world including Argentina, Bangladesh, Chile, China, India, Mexico, Thailand, United States of America and also in Brazil, specially in the Iron Quadrangle area, where mining activities have been contributing to aggravate natural contamination. Among other elements, iron is capable to interfere with the arsenic absorption by plants; iron ore has been proposed to remediate areas contaminated by the mentioned metalloid. In order to verify if iron can interfere with arsenic absorption by different taxa of plants, specimens of Brassicacea and Equisetaceae were kept in a 1/4 Murashige and Skoog basal salt solution (M and S), with 10 μgL -1 of arsenic acid. And varying concentrations of iron. The specimens were analysed by neutron activation analysis, k 0 -method, a routine technique in CDTN, and also very appropriate for arsenic studies. The preliminary results were quite surprising, showing that iron can interfere with arsenic absorption by plants, but in different ways, according to the species studied. (author)

  20. Dispensing processes impact apparent biological activity as determined by computational and statistical analyses.

    Directory of Open Access Journals (Sweden)

    Sean Ekins

    Full Text Available Dispensing and dilution processes may profoundly influence estimates of biological activity of compounds. Published data show Ephrin type-B receptor 4 IC50 values obtained via tip-based serial dilution and dispensing versus acoustic dispensing with direct dilution differ by orders of magnitude with no correlation or ranking of datasets. We generated computational 3D pharmacophores based on data derived by both acoustic and tip-based transfer. The computed pharmacophores differ significantly depending upon dispensing and dilution methods. The acoustic dispensing-derived pharmacophore correctly identified active compounds in a subsequent test set where the tip-based method failed. Data from acoustic dispensing generates a pharmacophore containing two hydrophobic features, one hydrogen bond donor and one hydrogen bond acceptor. This is consistent with X-ray crystallography studies of ligand-protein interactions and automatically generated pharmacophores derived from this structural data. In contrast, the tip-based data suggest a pharmacophore with two hydrogen bond acceptors, one hydrogen bond donor and no hydrophobic features. This pharmacophore is inconsistent with the X-ray crystallographic studies and automatically generated pharmacophores. In short, traditional dispensing processes are another important source of error in high-throughput screening that impacts computational and statistical analyses. These findings have far-reaching implications in biological research.

  1. Blood concentrations of ions and metals in amateur and elite runners using neutron activation analyses

    International Nuclear Information System (INIS)

    Santos, Luciana Kovacs dos

    2012-01-01

    In this study Br, Ca, Cl, Fe, I, K, Mg, Na, S and Zn concentration were investigated in blood of Brazilian athletes (endurance) using Neutron Activation Analyses technique (NAA). The blood samples were collected from male amateur athletes (AR) and male and female elite athletes (ER), ranging from 18 to 36 year old. The blood samples were collected at the LABEX/UNICAMP and they were irradiated in the nuclear reactor IEA-R1 at IPEN (Sao Paulo, Brazil). The range (at rest) established for AR and ER were compared with the control group (CG), subjects of same gender and age but not involved with physical activities, and showed significant differences for Ca (51 - 439 mgL -1 for CG, 162 - 410 mgL -1 for AR and 64 - 152 mgL -1 for ER) and Br (7.4 - 30.6 mgL -1 for CG, 4.0 - 9.6 mgL -1 for AR and 1.9 - 3.5 mgL -1 for ER), suggesting that a strong dependency of these limits in function of adopted physical training exists. We also performed a systematic investigation for the AR before, during and after the exercise program. These data can be considered for the preparation of a balanced diet, for evaluating the performance of the athletes during the period of competition preparation as well as contributing for proposing new protocols of clinical evaluation not reported in the literature yet. (author)

  2. Reproductive gonadal steroidogenic activity in the fishing cat (Prionailurus viverrinus) assessed by fecal steroid analyses.

    Science.gov (United States)

    Santymire, Rachel M; Brown, Janine L; Stewart, Rosemary A; Santymire, Robb C; Wildt, David E; Howard, JoGayle

    2011-10-01

    Non-invasive fecal steroid analyses were used to characterize gonadal activity in the fishing cat (Prionailurus viverrinus). Estrogen, progestagen and androgen metabolites were quantified in fecal samples collected for 12 months from four males and 10 females housed at seven North American zoological institutions. Male reproductive hormone concentrations did not vary (P>0.05) among season, and estrogen cycles were observed year-round in females and averaged (±SEM) 19.9±1.0 days. Mean peak estrogen concentration during estrus (460.0±72.6ng/g feces) was five-fold higher than baseline (87.3±14.0ng/g feces). Five of seven females (71.4%) housed alone or with another female demonstrated spontaneous luteal activity (apparent ovulation without copulation), with mean progestagen concentration (20.3±4.7μg/g feces), increasing nearly five-fold above baseline (4.1±0.8μg/g feces). The non-pregnant luteal phase averaged 32.9±2.5 days (n=13). One female delivered kittens 70 days after natural mating with fecal progestagen concentrations averaging 51.2±5.2μg/g feces. Two additional females were administered exogenous gonadotropins (150IU eCG; 100IU hCG), which caused hyper-elevated concentrations of fecal estrogen and progestagen (plus ovulation). Results indicate that: (1) male and female fishing cats managed in North American zoos are reproductively active year round; (2) 71.4% of females experienced spontaneous ovulation; and (3) females are responsive to exogenous gonadotropins for ovulation induction, but a regimen that produces a normative ovarian steroidogenic response needs to be identified. Copyright © 2011 Elsevier B.V. All rights reserved.

  3. The effect of innovative activity in firm performance and development: Analysing data from eurozone

    Directory of Open Access Journals (Sweden)

    Ilias A. Makris

    2016-06-01

    Full Text Available Purpose – The purpose of this paper is to examine the effect of Innovative Activity on firm performance and growth. Active Research and Development is considered to be directly related with development, prosperity and growth, in micro and macro level and a key factor in hindering economic recession. Design/methodology/approach – We analyse economic data from listed firms of selected eurozone country-members in order to associate Research and Development with performance indicators in firm and country level. For that purpose, several firm data were collected from WorldScope data base and macroeconomic data from Worldbank database. The period examined is between 2002 and 2012, with a special focus on current financial crisis (after 2007. The empirical process includes, descriptive statistics and logistic regression analysis. Findings – Findings indicate the crucial effect of innovative process in economic performance and development in firm and country level. The latter highlights the urgent need for public support in order to spur innovative activity and high-tech exports, especially in countries that were heavily affected by recession. Research limitations/implications – Some research limitations are the large number of missing cases in WordScope database, as many firms after the beginning of current crisis exit stock market. Furthermore, the other part of the economy, the Small and Medium Enterprises does not exist in the analysis, as listed firms are mainly large and mature companies. Originality/value – The results tend to highlight the need for common policy measures in eurozone, in regard to such issues, instead of imposing horizontal budgetary constraints in specific countries (like Southern Europe, hindering the vicious recessionary circle.

  4. Improved positron emission tomography imaging of glioblastoma cancer using novel 68Ga-labeled peptides targeting the urokinase-type plasminogen activator receptor (uPAR)

    DEFF Research Database (Denmark)

    Loft, Mathias Dyrberg; Sun, Yao; Liu, Changhao

    2017-01-01

    for non-invasive positron emission tomography (PET) imaging of uPAR. Despite the optimal physical properties of68Ga for peptide-based PET imaging, low tumor uptakes have previously been reported using68Ga-labeled AE105-NH2-based tracers. In an attempt to optimize the tumor uptake, we developed three novel...... to the non-spacer version, NODAGA-AE105-NH2. Following radiolabeling with68Ga, we evaluated the in vitro and in vivo performance in mice bearing subcutaneous tumors derived from the uPAR-expressing human GBM cell line U87MG. In vivo PET/CT imaging showed that introduction of PEG spacers more than doubled...... confirmed the improved tumor uptakes of the PEG-modified tracers.68Ga-NODAGA-PEG8-AE105-NH2is thus a promising candidate for human translation for PET imaging of GBM....

  5. Row orientation effect on UV-B, UV-A and PAR solar irradiation components in vineyards at Tuscany, Italy

    Science.gov (United States)

    Grifoni, D.; Carreras, G.; Zipoli, G.; Sabatini, F.; Dalla Marta, A.; Orlandini, S.

    2008-11-01

    Besides playing an essential role in plant photosynthesis, solar radiation is also involved in many other important biological processes. In particular, it has been demonstrated that ultraviolet (UV) solar radiation plays a relevant role in grapevines ( Vitis vinifera) in the production of certain important chemical compounds directly responsible for yield and wine quality. Moreover, the exposure to UV-B radiation (280-320 nm) can affect plant-disease interaction by influencing the behaviour of both pathogen and host. The main objective of this research was to characterise the solar radiative regime of a vineyard, in terms of photosynthetically active radiation (PAR) and UV components. In this analysis, solar spectral UV irradiance components, broadband UV (280-400 nm), spectral UV-B and UV-A (320-400 nm), the biological effective UVBE, as well as the PAR (400-700 nm) component, were all considered. The diurnal patterns of these quantities and the UV-B/PAR and UV-B/UV-A ratios were analysed to investigate the effect of row orientation of the vineyard in combination with solar azimuth and elevation angles. The distribution of PAR and UV irradiance at various heights of the vertical sides of the rows was also studied. The results showed that the highest portion of plants received higher levels of daily radiation, especially the UV-B component. Row orientation of the vines had a pronounced effect on the global PAR received by the two sides of the rows and, to a lesser extent, UV-A and UV-B. When only the diffused component was considered, this geometrical effect was greatly attenuated. UV-B/PAR and UV-A/PAR ratios were also affected, with potential consequences on physiological processes. Because of the high diffusive capacity of the UV-B radiation, the UV-B/PAR ratio was significantly lower on the plant portions exposed to full sunlight than on those in the shade.

  6. New developments in the analysis and measurement of thicknesses by excitation of the fluorescent lines by means of {beta} particles; Nouveaux developpements de l'analyse et de la mesure des epaisseurs par excitation des raies de fluorescence au moyen de particules {beta}

    Energy Technology Data Exchange (ETDEWEB)

    Martinelli, P [Commissariat a l' Energie Atomique, Saclay (France).Centre d' Etudes Nucleaires; Seibel, G [Institut de Recherches de la Siderurgie, 78 - Saint-Germain-en-Laye (France)

    1960-07-01

    The process for analysing and measuring the thickness of deposits by {beta}-X fluorescence which we have already described has undergone further development. The use of promethium-147 and krypton-85 sources makes it possible to reduce the background noise which is observed with strontium-90. We present the results obtained for various measurements of the thickness of metallic coatings and the continuous measurement of calcium and of iron in ore samples. We describe the tests carried out with a view to analysing the X-rays by means of a crystal. (author) [French] Le procede d'analyse et de mesure des epaisseurs de depots par fluorescence {beta}-X que nous avons precedemment decrit a fait l'objet de nouveaux developpements. L'emploi de sources de prometheum-147 et de krypton-85 permet de reduire le bruit de fond que l'on observe avec le strontium-90. Nous dormons les resultats obtenus pour diverses mesures d'epaisseurs de depots metalliques et la mesure en continu du calcium et du fer dans les carottes de minerais. Nous decrivons les essais effectues en vue d'analyser le rayonnement X au moyen d'un cristal. (auteur)

  7. Numerical and experimental analyses of different magnetic thermodynamic cycles with an active magnetic regenerator

    International Nuclear Information System (INIS)

    Plaznik, Uroš; Tušek, Jaka; Kitanovski, Andrej; Poredoš, Alojz

    2013-01-01

    We have analyzed the influence of different magnetic thermodynamic cycles on the performance of a magnetic cooling device with an active magnetic regenerator (AMR) based on the Brayton, Ericsson and Hybrid Brayton–Ericsson cycles. Initially, a numerical simulation was performed using a 1D, time-dependent, numerical model. Then a comparison was made with respect to the cooling power and the COP for different temperature spans. We showed that applying the Ericsson or the Hybrid Brayton–Ericsson cycle with an AMR, instead of the standard Brayton cycle, can increase the efficiency of the selected cooling device. Yet, in the case of the Ericsson cycle, the cooling power was decreased compared to the Hybrid and especially compared to the Brayton cycle. Next, an experimental analysis was carried out using a linear-type magnetic cooling device. Again, the Brayton, Ericsson and Hybrid Brayton–Ericsson cycles with an AMR were compared with respect to the cooling power and the COP for different temperature spans. The results of the numerical simulation were confirmed. The Hybrid Brayton–Ericsson cycle with an AMR showed the best performance if a no-load temperature span was considered as a criterion. -- Highlights: • New thermodynamic cycles with an active magnetic regenerator (AMR) are presented. • Three different thermodynamic cycles with an AMR were analyzed. • Numerical and experimental analyses were carried out. • The best overall performance was achieved with the Hybrid Brayton–Ericsson cycle. • With this cycle the temperature span of test device was increased by almost 10%

  8. Selective killing of cancer cells by leaf extract of Ashwagandha: components, activity and pathway analyses.

    Science.gov (United States)

    Widodo, Nashi; Takagi, Yasuomi; Shrestha, Bhupal G; Ishii, Tetsuro; Kaul, Sunil C; Wadhwa, Renu

    2008-04-08

    Ashwagandha, also called as "Queen of Ayurveda" and "Indian ginseng", is a commonly used plant in Indian traditional medicine, Ayurveda. Its roots have been used as herb remedy to treat a variety of ailments and to promote general wellness. However, scientific evidence to its effects is limited to only a small number of studies. We had previously identified anti-cancer activity in the leaf extract (i-Extract) of Ashwagandha and demonstrated withanone as a cancer inhibitory factor (i-Factor). In the present study, we fractionated the i-Extract to its components by silica gel column chromatography and subjected them to cell based activity analyses. We found that the cancer inhibitory leaf extract (i-Extract) has, at least, seven components that could cause cancer cell killing; i-Factor showed the highest selectivity for cancer cells and i-Factor rich Ashwagandha leaf powder was non-toxic and anti-tumorigenic in mice assays. We undertook a gene silencing and pathway analysis approach and found that i-Extract and its components kill cancer cells by at least five different pathways, viz. p53 signaling, GM-CFS signaling, death receptor signaling, apoptosis signaling and G2-M DNA damage regulation pathway. p53 signaling was most common. Visual analysis of p53 and mortalin staining pattern further revealed that i-Extract, fraction F1, fraction F4 and i-Factor caused an abrogation of mortalin-p53 interactions and reactivation of p53 function while the fractions F2, F3, F5 work through other mechanisms.

  9. Analysis of radionuclide mixtures by {alpha}-{gamma} and {beta}-{gamma} coincidences using a simple device; Analyse de melanges de radionucleides par un dispositif simple de coincidences {alpha}-{gamma} et {beta}-{gamma}

    Energy Technology Data Exchange (ETDEWEB)

    Pottier, R; Berger, R [Commissariat a l' Energie Atomique, Centre d' Etudes Nucleaires de Fontenay-aux-Roses, 92 (France)

    1966-06-01

    A procedure is described for the qualitative and quantitative spectrographic analysis of radioactive sources containing two alpha-gamma emitters having the same alpha energy or two beta-gamma emitters having the same gamma energy. The main apparatus is a multichannel pulse-height analyzer including a coincidence circuit. The principle of the method, the synoptic scheme, the electronic device, the type of sources, and the precautions to be taken or the corrections to take into account are reported. The results obtained in solving the three following problems are discussed as examples of applications of the method: analysis of {sup 241}Am in alpha-gamma sources containing {sup 238}Pu; analysis of {sup 237}Np in beta-gamma sources containing {sup 239}Pu; and analysis of {sup 106}Ru-{sup 106}Rh in beta-gamma sources containing {sup 95}Zr-{sup 95}Nb. (authors) [French] Dans ce. rapport, on presente une methode d'analyse spectrographique qualitative et quantitative de sources radioactives contenant deux emetteurs alpha-gamma de meme energie alpha et deux emetteurs beta-gamma de meme energie gamma. L'organe principal est un analyseur d'amplitude a 400 canaux comprenant un circuit de coincidence. On decrit le principe de la methode, le schema synoptique, l'appareillage, le type des sources, les precautions a prendre ou les corrections a faire. On discute les resultats obtenus dans la solution des trois problemes suivants traites a titre d'application de la methode: 1. analyse d'americium 241 en presence de plutonium 238; 2. analyse de neptunium 237 en presence de plutonium 239; 3. analyse de ruthenium 106-rhodium 106 en presence de zirconium 95-niobium 95. (auteurs)

  10. Utilisation de l'analyse sémantique latente pour tenter d'optimiser l'acquisition par exposition à une langue étrangère de spécialité

    Directory of Open Access Journals (Sweden)

    Virginie Zampa

    2005-09-01

    Full Text Available Cet article présente l'utilisation de l'analyse sémantique latente (Latent Semantic Analysis dans un prototype d'acquisition de langue étrangère de spécialité. Ce prototype nommé Rafales (Recueil Automatique Favorisant l'Acquisition d'une Langue Étrangère de Spécialité a pour finalité d'optimiser l'acquisition d'une langue étrangère en fournissant des lectures à l'apprenant. Les textes fournis à l'apprenant dépendent de ses connaissances ainsi que des connaissances du domaine de spécialité. L'article est divisé en trois parties, la première présente l'analyse sémantique latente, son fonctionnement et ses utilisations. La seconde partie présente Rafales, son architecture et son fonctionnement. Enfin la dernière partie présente les résultats de l'expérimentation du prototype auprès d'apprenants ainsi que les raisons qui nous ont poussées à utiliser cette analyse dans Rafales.

  11. Analysis of resonances due to 'S' neutrons in experiments on transmission by time-of-flight. 1. without the interference term; Analyse des resonances dues aux neutrons 'S' dans les experiences de transmission par temps de vol. 1. sans terme d'interference

    Energy Technology Data Exchange (ETDEWEB)

    Corge, C R [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

    1961-07-01

    Various methods of transmission resonance analysis for 's' wave neutrons in time of flight experiments are described in this report. Only the case when the interference term can be neglected is treated in this first part. Results are given relative to a more complete partial area method and also to a new method so-called the width at half depth method. (author) [French] Le present rapport est consacre a l'expose de differentes methodes d'analyse des resonances dues aux neutrons d'ondes 's' dans les experiences de transmission par temps de vol, cette premiere partie traitant uniquement le cas ou l'on peut negliger le terme d'interference. Y figurent en particulier sous forme de reseaux de courbes, les resultats relatifs a une methode des aires partielles amelioree et ceux relatifs a une nouvelle methode dite de la largeur a mi-profondeur. (auteur)

  12. The Estimation of Internal Contamination with Uranium from Urine Analysis Results; Evaluation de la Contamination Interne par l'Uranium a Partir des Resultats de l'Analyse d'Urines; 041e 0426 0414 ; Evaluacion de la Contaminacion Interna con Uranio a Partir de los Resultados del Analisis de Orina

    Energy Technology Data Exchange (ETDEWEB)

    Jackson, S. [Authority Health and Safety Branch, Radiological Protection Division, United Kingdom Atomic Energy Authority, Harwell, Berks. (United Kingdom)

    1964-11-15

    The most common cause of internal contamination with uranium is inhalation of uranium dust. Soluble uranium compounds are rapidly absorbed from the lungs, and most of the uranium entering the circulation is rapidly excreted in the urine. The intake of soluble uranium at work can be estimated from the uranium content of urine samples taken immediately afterwards. It is a special feature of soluble compounds of natural uranium that the primary consideration is not radiological but toxicological due to chemical effects of uranium deposited in the kidneys. Some uranium is also deposited in the skeleton, but the irradiation of bone is less critical, in the case of natural uranium, than the toxicity to kidney. For soluble compounds of highly-enriched uranium with its high specific activity, the primary concern is radiological; the chemical effects on the kidneys are less limiting than the irradiation of bone, which thus becomes the critical organ. In this case, it is better to attempt to assess the retained body burden rather than the intake of uranium. This may be done by analysing urine samples taken not immediately after exposure but when some time has elapsed. Samples taken after a holiday are probably the best material available in practice, but it may be necessary to accept samples after only a week-end of removal from uranium work. (author) [French] Evaluation de la contamination interne par l'uranium a partir des resultats de l'analyse d'urines. La cause la plus frequente de contamination interne par l'uranium est l'inhalation de poussieres d'uranium. Les composes solubles de l'uranium sont rapidement absorbes au niveau des poumons et la plus grande partie de l'uranium qui passe dans le sang est rapidement rejetee avec l'urine. La quantite d'uranium soluble actif qui est absorbee peut etre determinee a partir de la teneur en uranium d'echantillons preleves immediatement apres. Les composes solubles de l'uranium naturel ont une particularite en ce sens que la

  13. Absolute measurement of a standard thermal-neutron flux using gold-detector activation; Mesure absolue d'un flux etalon de neutrons thermiques par activation de detecteurs d'or

    Energy Technology Data Exchange (ETDEWEB)

    Paternot, Y [Commissariat a l' Energie Atomique, Fontenay-aux-Roses (France). Centre d' Etudes Nucleaires

    1967-02-15

    The density of neutrons in a standard stacking is determined between the zero-energy and the cut-off energy of 1 mm thick cadmium unit using a gold detector. Its absolute activity is measured using a 4 {pi} {beta} counter calibrated for 4 {pi} {beta},{gamma} coincidence by counting strongly active sources. The correction factor F due to the disintegration process for the gold is determined experimentally. {phi}{sub 0} = N{sub 0} {sup E}Cd{sub V0} = 6495 {+-} 1.5 per cent n/cm{sup 2}/s. (author) [French] La densite de neutrons dans un empilement etalon est determinee entre l'energie zero et l'energie de coupure d'un boitier de cadmium de 1 mm d'epaisseur au moyen d'un detecteur d'or. Son activite absolue est mesuree a l'aide d'un compteur 4 {pi} {beta} etalonne en coincidence 4 {pi} {beta},{gamma} par comptage de sources fortement actives. Le facteur de correction F d au schema de desintegration de l'or est determine experimentalement. {phi}{sub 0} = N{sub 0} {sup E}Cd{sub V0} = 6495 {+-} 1.5 pour cent n/cm{sup 2}/s. (auteur)

  14. Fissile materials in solution concentration measured by active neutron interrogation; Mesure de concentration en matiere fissile dans les liquides par interrogation neutronique active

    Energy Technology Data Exchange (ETDEWEB)

    Romeyer Dherbey, J.; Passard, Ch.; Cloue, J.; Bignan, G.

    1993-12-31

    The use of the active neutron interrogation to measure the concentration of plutonium contained in flow solutions is particularly interesting for fuel reprocessing plants. Indeed, this method gives a signal which is in a direct relation with the fissile materials concentration. Moreover, it is less sensitive to the gamma dose rate than the other nondestructive methods. Two measure methods have been evolved in CEA. Their principles are given into details in this work. The first one consists to detect fission delayed neutrons induced by a {sup 252} Cf source. In the second one fission prompt neutrons induced by a neutron generator of 14 MeV are detected. (O.M.). 6 refs.

  15. Recent activity on the post-irradiation analyses of nuclear fuels and actinide samples at JAERI

    International Nuclear Information System (INIS)

    Shinohara, Nobuo; Nakahara, Yoshinori; Kohno, Nobuaki; Tsujimoto, Kazufumi

    2003-01-01

    Radiochemical analyses of spent fuels have been carried out at JAERI for contributing to the development of nuclear technologies, where several samples from research reactors and nuclear power plants were analyzed to obtain isotopic compositions and burnups. The history and procedures of the radiochemical analyses are depicted and some recent results are given in this paper. (author)

  16. Parálisis cerebral :

    OpenAIRE

    Cabrero Izquierdo, María del Carmen

    2012-01-01

    Se aborda el tema de la parálisis cerebral definiendo qué es, clasificando los tipos de parálisis dependiendo de la afectación y las características principales. Se explican algunos de sus tratamientos, se dan sistemas alternativos y/o aumentativos de comunicación para un alumno con PC (parálisis cerebral).

  17. Usage pédagogique de Facebook dans une activité d’apprentissage en groupe par des étudiants tunisiens : analyse de l’efficacité du travail collaboratif

    Directory of Open Access Journals (Sweden)

    Hassen BEN REBAH

    2017-01-01

    Full Text Available Cet article présente une analyse de l’usage pédagogique du réseau socio-numérique Facebook comme dispositif d’apprentissage collaboratif à distance dans l’enseignement supérieur, notamment à l’Institut supérieur des études technologiques de Mahdia (Tunisie. Cette recherche qualitative vise à évaluer l’efficacité du travail collaboratif dans le cadre d’une activité d’apprentissage impliquant des étudiants en informatique. Nous tentons à cet effet de déterminer en quoi l’usage de Facebook comme outil de partage, de communication et d’échanges en réseau favoriserait la mise en place d’un processus efficace de co-construction des savoirs et d’interactions entre apprenants. Pour atteindre cet objectif, l’analyse s’appuie sur le paradigme de l’apprentissage social de Bandura (1977 qui met l’accent sur le jeu d’interactions permanentes entre les comportements d’un individu, son fonctionnement cognitif et les variables de l’environnement. Les données issues des traces d’activités sur le mur du groupe Facebook et des contenus d’entretiens semi-directifs ont permis après analyse d’établir que l’utilisation pédagogique de Facebook a effectivement favorisé la coproduction des savoirs attendus chez les étudiants au cours de l’activité. Ainsi, nous sommes parvenus à la conclusion que l’usage de Facebook est efficace pour la mise en place d’une activité d’apprentissage collaborative à distance. Cependant, cela nécessite l’élaboration en amont d’un véritable scénario de formation organisant le déroulement de l’activité.

  18. Histamine H3 receptor activation selectively inhibits dopamine D1 receptor-dependent [3H]GABA release from depolarization-stimulated slices of rat substantia nigra pars reticulata

    International Nuclear Information System (INIS)

    Aceves, J.; Young, J.M.; Arias-Montano, J.A.; Floran, B.; Garcia, M.

    1997-01-01

    The release of [ 3 H]GABA from slices of rat substantia nigra pars reticulata induced by increasing extracellular K + from 6 to 15 mM in the presence of 10 μM sulpiride was inhibited by 73±3% by 1 μM SCH 23390, consistent with a large component of release dependent upon D 1 receptor activation. The histamine H 3 receptor-selective agonist immepip (1 μM) and the non-selective agonist histamine (100 μM) inhibited [ 3 H]GABA release by 78±2 and 80±2%, respectively. The inhibition by both agonists was reversed by the H 3 receptor antagonist thioperamide (1 μM). However, in the presence of 1 μM SCH 23390 depolarization-induced release of [ 3 H]GABA was not significantly decreased by 1 μM immepip. In rats depleted of dopamine by pretreatment with reserpine, immepip no longer inhibited control release of [ 3 H]GABA, but in the presence of 1 μM SKF 38393, which produced a 7±1-fold stimulation of release, immepip reduced the release to a level not statistically different from that in the presence of immepip alone. Immepip (1 μM) also inhibited the depolarization-induced release of [ 3 H]dopamine from substantia nigra pars reticulata slices, by 38±3%.The evidence is consistent with the proposition that activation of histamine H 3 receptors leads to the selective inhibition of the component of depolarization-induced [ 3 H]GABA release in substantia nigra pars reticulata slices which is dependent upon D 1 receptor activation. This appears to be largely an action at the terminals of the striatonigral GABA projection neurons, which may be enhanced by a partial inhibition of dendritic [ 3 H]dopamine release. (Copyright (c) 1997 Elsevier Science B.V., Amsterdam. All rights reserved.)

  19. Non-destructive analysis and detection of internal characteristics of spruce logs through X computerized tomography; Detection et analyse non destructive de caracteristiques internes de billons d'epicea commun (PICEA ABIES (L.) KARST) par tomographie a rayons X

    Energy Technology Data Exchange (ETDEWEB)

    Longuetaud, F

    2005-10-15

    Computerized tomography allows a direct access to internal features of scanned logs on the basis of density and moisture content variations. The objective of this work is to assess the feasibility of an automatic detection of internal characteristics with the final aim of conducting scientific analyses. The database is constituted by CT images of 24 spruces obtained with a medical CT scanner. Studied trees are representative of several social status and are coming from four stands located in North-Eastern France, themselves are representative of several age, density and fertility classes. The automatic processing developed are the following. First, pith detection in logs dealing with the problem of knot presence and ring eccentricity. The accuracy of the localisation was less than one mm. Secondly, the detection of the sapwood/heart-wood limit in logs dealing with the problem of knot presence (main source of difficulty). The error on the diameter was 1.8 mm which corresponds to a relative error of 1.3 per cent. Thirdly, the detection of the whorls location and comparison with an optical method. Fourthly the detection of individualized knots. This process allows to count knots and to locate them in a log (longitudinal position and azimuth); however, the validation of the method and extraction of branch diameter and inclination are still to be developed. An application of this work was a variability analysis of the sapwood content in the trunk: at the within-tree level, the sapwood width was found to be constant under the living crown; at the between-tree level, a strong correlation was found with the amount of living branches. A great number of analyses are possible from our work results, among others: architectural analysis with the pith tracking and the apex death occurrence; analysis of radial variations of the heart-wood shape; analysis of the knot distribution in logs. (author)

  20. Non-destructive analysis and detection of internal characteristics of spruce logs through X computerized tomography; Detection et analyse non destructive de caracteristiques internes de billons d'epicea commun (PICEA ABIES (L.) KARST) par tomographie a rayons X

    Energy Technology Data Exchange (ETDEWEB)

    Longuetaud, F

    2005-10-15

    Computerized tomography allows a direct access to internal features of scanned logs on the basis of density and moisture content variations. The objective of this work is to assess the feasibility of an automatic detection of internal characteristics with the final aim of conducting scientific analyses. The database is constituted by CT images of 24 spruces obtained with a medical CT scanner. Studied trees are representative of several social status and are coming from four stands located in North-Eastern France, themselves are representative of several age, density and fertility classes. The automatic processing developed are the following. First, pith detection in logs dealing with the problem of knot presence and ring eccentricity. The accuracy of the localisation was less than one mm. Secondly, the detection of the sapwood/heart-wood limit in logs dealing with the problem of knot presence (main source of difficulty). The error on the diameter was 1.8 mm which corresponds to a relative error of 1.3 per cent. Thirdly, the detection of the whorls location and comparison with an optical method. Fourthly the detection of individualized knots. This process allows to count knots and to locate them in a log (longitudinal position and azimuth); however, the validation of the method and extraction of branch diameter and inclination are still to be developed. An application of this work was a variability analysis of the sapwood content in the trunk: at the within-tree level, the sapwood width was found to be constant under the living crown; at the between-tree level, a strong correlation was found with the amount of living branches. A great number of analyses are possible from our work results, among others: architectural analysis with the pith tracking and the apex death occurrence; analysis of radial variations of the heart-wood shape; analysis of the knot distribution in logs. (author)

  1. Analyze of phase's mechanical behaviour of a multiphase polycrystalline alloy by X-ray and neutron diffraction; Analyse du comportement mecanique des constituants d'un alliage polycristallin multiphase par diffraction des rayons X et neutronique

    Energy Technology Data Exchange (ETDEWEB)

    Dakhlaoui, R

    2006-12-15

    The aim of this work is to propose a methodology using diffraction methods and theoretical approaches of self-consistent modeling in order to analyze and better understand the mechanical behavior of each phase of hot-rolled duplex stainless steel. The purpose of the experimental study is to characterize the local mechanical behavior of phases under uniaxial loading. X-ray and neutron diffraction which enable to measure strains in each phase separately were used in this aim. Austenitic and ferritic phase stresses are determined by X-ray diffraction during tensile tests. Evolution of the elastic strains in each phase was measured by neutron diffraction using 'time-of-flight' method during tensile and compression tests. Elastic constants were given using the self-consistent model for a purely elastic deformation. To reproduce the mechanical behaviour of the studied material, self-consistent polycrystalline micro-mechanical model for elastoplastic deformation has been adapted and confronted to experimental results. Crystallographic texture and initial residual stresses were considered in this analysis. Critical shear stresses and hardening parameters of each phase of the studied duplex steel have been identified. Results of this study showed that the austenitic phase represents the softest and the most hardenable phase. Taking into account in calculations the initial residual stresses in the non deformed sample leads to the conclusion that the initial stresses modify considerably the values of phase's yield stresses. Good agreement has been noted comparing results obtained by XRD to those obtained by neutron diffraction. The problem of relaxation of normal stresses in the analysed layer by X-rays was analysed and discussed. Using XRD and self-consistent modelling, the effect of the chemical composition of the duplex stainless steel and the influence of ageing at 400 C degrees for 1000 h on the mechanical behaviour of austenitic and ferritic phases have

  2. Par3L enhances colorectal cancer cell survival by inhibiting Lkb1/AMPK signaling pathway

    International Nuclear Information System (INIS)

    Li, Taiyuan; Liu, Dongning; Lei, Xiong; Jiang, Qunguang

    2017-01-01

    Partitioning defective 3-like protein (Par3L) is a recently identified cell polarity protein that plays an important role in mammary stem cell maintenance. Previously, we showed that high expression of Par3L is associated with poor survival in malignant colorectal cancer (CRC), but the underlying mechanism remained unknown. To this end, we established a Par3L knockout colorectal cancer cell line using the CRISPR/Cas system. Interestingly, reduced proliferation, enhanced cell death and caspase-3 activation were observed in Par3L knockout (KO) cells as compared with wildtype (WT) cells. Consistent with previous studies, we showed that Par3L interacts with a tumor suppressor protein liver kinase B1 (Lkb1). Moreover, Par3L depletion resulted in abnormal activation of Lkb1/AMPK signaling cascade. Knockdown of Lkb1 in these cells could significantly reduce AMPK activity and partially rescue cell death caused by Par3L knockdown. Furthermore, we showed that Par3L KO cells were more sensitive to chemotherapies and irradiation. Together, these results suggest that Par3L is essential for colorectal cancer cell survival by inhibiting Lkb1/AMPK signaling pathway, and is a putative therapeutic target for CRC. - Highlights: • Par3L knockout using the CRISPR/Cas system induces apoptosis in colorectal cancer cells. • Par3L interacts with Lkb1 and regulates the activity of AMPK signaling cascade. • Par3L knockout cells are more sensitive to treatment of different chemotherapy drugs and irradiation.

  3. ‘Actionner’ l’intégration par le discours – une analyse de la problématique des actes de langage dans le discours politique de l’intégration

    Directory of Open Access Journals (Sweden)

    Raluca-Nicoleta Balatchi

    2007-03-01

    Full Text Available The paper aims to discuss the importance of speech acts in a particular type of political discourse, namely the “integration discourse” from two opposite viewpoints: that of the politician whose country looks forward to being integrating and that of the politician whose country integrates, situates therefore at the other end of the same axis of interest. It suggests that this type of discourse is mainly an active one, as its purpose being is, given the necessary conditions, to create a reality rather than describe an existing one, even, if necessary, by manipulating the audience according to the speaker’s interests.

  4. Theoretical study of the electronic structure of f-element complexes by quantum chemical methods; Analyse de la structure electronique des complexes contenant des elements F par des methodes de la chimie quantique

    Energy Technology Data Exchange (ETDEWEB)

    Vetere, V

    2002-09-15

    This thesis is related to comparative studies of the chemical properties of molecular complexes containing lanthanide or actinide trivalent cations, in the context of the nuclear waste disposal. More precisely, our aim was a quantum chemical analysis of the metal-ligand bonding in such species. Various theoretical approaches were compared, for the inclusion of correlation (density functional theory, multiconfigurational methods) and of relativistic effects (relativistic scalar and 2-component Hamiltonians, relativistic pseudopotentials). The performance of these methods were checked by comparing computed structural properties to published experimental data, on small model systems: lanthanide and actinide tri-halides and on X{sub 3}M-L species (X=F, Cl; M=La, Nd, U; L = NH{sub 3}, acetonitrile, CO). We have thus shown the good performance of density functionals combined with a quasi-relativistic method, as well as of gradient-corrected functionals associated with relativistic pseudopotentials. In contrast, functionals including some part of exact exchange are less reliable to reproduce experimental trends, and we have given a possible explanation for this result . Then, a detailed analysis of the bonding has allowed us to interpret the discrepancies observed in the structural properties of uranium and lanthanides complexes, based on a covalent contribution to the bonding, in the case of uranium(III), which does not exist in the lanthanide(III) homologues. Finally, we have examined more sizeable systems, closer to experimental species, to analyse the influence of the coordination number, of the counter-ions and of the oxidation state of uranium, on the metal-ligand bonding. (author)

  5. Brain imaging and memory systems in humans: the contribution of PET methods; Imagerie cerebrale et systeme de memoire chez l'homme: contribution de l'analyse par TEP

    Energy Technology Data Exchange (ETDEWEB)

    Perani, D. [Institute of Neuroscience and Bioimaging CNR, Milan (Italy)

    1998-03-01

    The development of neuroimaging methods such as PET, has provided a new impulse to the study of the neural basis of cognitive functions, and has extended the field of inquiry from the analysis of the consequences of brain lesions to the functional investigations of brain activity, either in patients with selective neuropsychological deficits or in normal subjects engaged in cognitive tasks. Specific patterns of hypo-metabolism in neurological patients are associated with different profiles of memory deficits.[{sup 18}F]FDG PET studies have confirmed the association of episodic memory with the structures of Papez's circuit and have shown correlations between short-term and semantic memory and the language areas. The identification of anatomical-functional networks involved in specific components of memory function in normal subjects is the aim of several PET activation studies. The results are in agreement with 'neural network' models of the neural basis of memory, as complex functions subserved by multiple interconnected cortical and subcortical structures. (author)

  6. Preliminary Results of Ancillary Safety Analyses Supporting TREAT LEU Conversion Activities

    Energy Technology Data Exchange (ETDEWEB)

    Brunett, A. J. [Argonne National Lab. (ANL), Argonne, IL (United States); Fei, T. [Argonne National Lab. (ANL), Argonne, IL (United States); Strons, P. S. [Argonne National Lab. (ANL), Argonne, IL (United States); Papadias, D. D. [Argonne National Lab. (ANL), Argonne, IL (United States); Hoffman, E. A. [Argonne National Lab. (ANL), Argonne, IL (United States); Kontogeorgakos, D. C. [Argonne National Lab. (ANL), Argonne, IL (United States); Connaway, H. M. [Argonne National Lab. (ANL), Argonne, IL (United States); Wright, A. E. [Argonne National Lab. (ANL), Argonne, IL (United States)

    2015-10-01

    The Transient Reactor Test Facility (TREAT), located at Idaho National Laboratory (INL), is a test facility designed to evaluate the performance of reactor fuels and materials under transient accident conditions. The facility, an air-cooled, graphite-moderated reactor designed to utilize fuel containing high-enriched uranium (HEU), has been in non-operational standby status since 1994. Currently, in support of the missions of the Department of Energy (DOE) National Nuclear Security Administration (NNSA) Material Management and Minimization (M3) Reactor Conversion Program, a new core design is being developed for TREAT that will utilize low-enriched uranium (LEU). The primary objective of this conversion effort is to design an LEU core that is capable of meeting the performance characteristics of the existing HEU core. Minimal, if any, changes are anticipated for the supporting systems (e.g. reactor trip system, filtration/cooling system, etc.); therefore, the LEU core must also be able to function with the existing supporting systems, and must also satisfy acceptable safety limits. In support of the LEU conversion effort, a range of ancillary safety analyses are required to evaluate the LEU core operation relative to that of the existing facility. These analyses cover neutronics, shielding, and thermal hydraulic topics that have been identified as having the potential to have reduced safety margins due to conversion to LEU fuel, or are required to support the required safety analyses documentation. The majority of these ancillary tasks have been identified in [1] and [2]. The purpose of this report is to document the ancillary safety analyses that have been performed at Argonne National Laboratory during the early stages of the LEU design effort, and to describe ongoing and anticipated analyses. For all analyses presented in this report, methodologies are utilized that are consistent with, or improved from, those used in analyses for the HEU Final Safety Analysis

  7. Determination by neutron activation of the uranium-235 concentration in uranium oxides; Determination par activation neutronique de la concentration d'uranium-235 dans des oxydes d'urane

    Energy Technology Data Exchange (ETDEWEB)

    May, S; Leveque, P [Commissariat a l' Energie Atomique, Saclay (France).Centre d' Etudes Nucleaires

    1959-07-01

    Classical methods of measuring isotopic abundance have the disadvantage of being long and of requiring chemical separation. A non-destructive method of measuring the uranium-235 content is described. It is based on an overall measurement of the short lived fission product activity formed during a 15 s neutron irradiation. The precision is of the order {+-} 1.5 per cent for 20 per cent enriched samples. The error due to the contribution from fast fission is discussed in detail. (author) [French] Les methodes classiques de mesure de l'abondance isotopique presentent le gros inconvenient d'etre longues et de necessiter des separations chimiques. Nous exposons une methode non destructive de mesure de la concentration d'uranium-235. Elle est basee sur la mesure globale de l'activite des produits de fission de courte periode formes par une irradiation neutronique de 15 s de l'echantillon. La precision est de l'ordre de {+-} 1,5 pour cent pour des echantillons enrichis jusqu'a 20 pour cent. L'erreur a la contribution de la fission rapide est discutee en detail. (auteur)

  8. How Effective Are Active Videogames Among the Young and the Old? Adding Meta-analyses to Two Recent Systematic Reviews.

    Science.gov (United States)

    van 't Riet, Jonathan; Crutzen, Rik; Lu, Amy Shirong

    2014-10-01

    Two recent systematic reviews have surveyed the existing evidence for the effectiveness of active videogames in children/adolescents and in elderly people. In the present study, effect sizes were added to these systematic reviews, and meta-analyses were performed. All reviewed studies were considered for inclusion in the meta-analyses, but only studies were included that investigated the effectiveness of active videogames, used an experimental design, and used actual health outcomes as the outcome measures (body mass index for children/adolescents [k=5] and functional balance for the elderly [k=6]). The average effect of active videogames in children and adolescents was small and nonsignificant: Hedges' g=0.20 (95 percent confidence interval, -0.08 to 0.48). Limited heterogeneity was observed, and no moderator analyses were performed. For the effect of active videogames on functional balance in the elderly, the analyses revealed a medium-sized and significant effect of g=0.68 (95 percent confidence interval, 0.13-1.24). For the elderly studies, substantial heterogeneity was observed. Moderator analyses showed that there were no significant effects of using a no-treatment control group versus an alternative treatment control group or of using games that were especially created for health-promotion purposes versus off-the-shelf games. Also, intervention duration and frequency, sample size, study quality, and dropout did not significantly moderate the effect of active videogames. The results of these meta-analyses provide preliminary evidence that active videogames can have positive effects on relevant outcome measures in children/adolescents and elderly individuals.

  9. Automation on computers of the partial area method for the analysis of 's' neutron induced resonances. I. Without interference terms. (1962); Automatisation sur ordinateur de la methode des aires partielle dans l'analyse des resonances induites par les neutrons 's'. I. sans terme d'interference. (1962)

    Energy Technology Data Exchange (ETDEWEB)

    Bianchi, G; Corge, C R [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

    1962-07-01

    This report deals with numerical analysis of transmission resonances induced by 's' wave neutrons in time of flight experiments. The analysis method used is the partial area one. The only case treated here, in this first part, is the one when the interference term can be neglected. Programs and subroutines are thoroughly described, which determine from experimental raw data the resonant transmission, the partial areas, and the resonance parameters. (authors) [French] Le present rapport a pour objet l'analyse numerique sur ordinateur IBM 7090, des resonances dues aux neutrons 's' dans les experiences de transmission par temps de vol, la methode d'analyse utilisee etant la methode des aires partielles. Dans cette premiere partie, seul a ete envisage le cas ou le terme d'interference peut etre neglige. On y trouvera une description detaillee des programmes et sous programmes elabores pour determiner les transmissions resonantes avec leur trace, a partir des donnees experimentales brutes, les aires partielles afferentes ainsi que les parametres caracteristiques des resonances. (auteurs)

  10. Contribution of stimulations for the optimization of quantitative electron probe micro analysis of heterogeneous catalysts; Apport de la simulation dans l'optimisation de l'analyse quantitative par microsonde electronique de catalyseurs heterogenes

    Energy Technology Data Exchange (ETDEWEB)

    Sorbier, L.

    2001-11-01

    Electron Probe Micro Analysis (EPMA) is frequently used to measure the local concentration of active elements in heterogeneous catalysts. However, when classical procedures are used, a significant deficit is observed both in local total concentration and mean total concentrations. A Monte Carlo program simulating measured intensities (characteristic lines and continuous background) has been written using PENELOPE routines. We have included in this program models taking into account the different physical phenomena likely to lead to the observed signal loss (insulating properties, roughness, porosity, energy loss at interfaces). Simulation results have shown that an important roughness (Ra>200 nm) was the only parameter apt to lead to a significant total signal loss. This led us to inquire into another origin to explain the signal loss observed on meso-porous samples. Measurements conducted on a meso-porous alumina confirmed that measuring aluminum, oxygen and carbon leads to a correct total of concentrations. Signal loss is thus explained by the contamination of the sample during its preparation, the components of the embedding resin diffusing into the porosity and reacting with the reactive surface of the catalyst support. In the case of macroporous catalysts, local roughness effect is very important. The simulations have shown the efficiency of the Peak to Background method to correct these local roughness effects. Measurements conducted on reforming and hydro-treating catalysts have led to a correct total concentration and confirmed the contribution of the Peak to Background method to achieve local quantitative measurement. (author)

  11. Contribution of stimulations for the optimization of quantitative electron probe micro analysis of heterogeneous catalysts; Apport de la simulation dans l'optimisation de l'analyse quantitative par microsonde electronique de catalyseurs heterogenes

    Energy Technology Data Exchange (ETDEWEB)

    Sorbier, L

    2001-11-01

    Electron Probe Micro Analysis (EPMA) is frequently used to measure the local concentration of active elements in heterogeneous catalysts. However, when classical procedures are used, a significant deficit is observed both in local total concentration and mean total concentrations. A Monte Carlo program simulating measured intensities (characteristic lines and continuous background) has been written using PENELOPE routines. We have included in this program models taking into account the different physical phenomena likely to lead to the observed signal loss (insulating properties, roughness, porosity, energy loss at interfaces). Simulation results have shown that an important roughness (Ra>200 nm) was the only parameter apt to lead to a significant total signal loss. This led us to inquire into another origin to explain the signal loss observed on meso-porous samples. Measurements conducted on a meso-porous alumina confirmed that measuring aluminum, oxygen and carbon leads to a correct total of concentrations. Signal loss is thus explained by the contamination of the sample during its preparation, the components of the embedding resin diffusing into the porosity and reacting with the reactive surface of the catalyst support. In the case of macroporous catalysts, local roughness effect is very important. The simulations have shown the efficiency of the Peak to Background method to correct these local roughness effects. Measurements conducted on reforming and hydro-treating catalysts have led to a correct total concentration and confirmed the contribution of the Peak to Background method to achieve local quantitative measurement. (author)

  12. How Do We Know What Is Happening Online?: A Mixed Methods Approach to Analysing Online Activity

    Science.gov (United States)

    Charalampidi, Marina; Hammond, Michael

    2016-01-01

    Purpose: The purpose of this paper is to discuss the process of analysing online discussion and argue for the merits of mixed methods. Much research of online participation and e-learning has been either message-focused or person-focused. The former covers methodologies such as content and discourse analysis, the latter interviewing and surveys.…

  13. ParABS Systems of the Four Replicons of Burkholderia cenocepacia: New Chromosome Centromeres Confer Partition Specificity†

    Science.gov (United States)

    Dubarry, Nelly; Pasta, Franck; Lane, David

    2006-01-01

    Most bacterial chromosomes carry an analogue of the parABS systems that govern plasmid partition, but their role in chromosome partition is ambiguous. parABS systems might be particularly important for orderly segregation of multipartite genomes, where their role may thus be easier to evaluate. We have characterized parABS systems in Burkholderia cenocepacia, whose genome comprises three chromosomes and one low-copy-number plasmid. A single parAB locus and a set of ParB-binding (parS) centromere sites are located near the origin of each replicon. ParA and ParB of the longest chromosome are phylogenetically similar to analogues in other multichromosome and monochromosome bacteria but are distinct from those of smaller chromosomes. The latter form subgroups that correspond to the taxa of their hosts, indicating evolution from plasmids. The parS sites on the smaller chromosomes and the plasmid are similar to the “universal” parS of the main chromosome but with a sequence specific to their replicon. In an Escherichia coli plasmid stabilization test, each parAB exhibits partition activity only with the parS of its own replicon. Hence, parABS function is based on the independent partition of individual chromosomes rather than on a single communal system or network of interacting systems. Stabilization by the smaller chromosome and plasmid systems was enhanced by mutation of parS sites and a promoter internal to their parAB operons, suggesting autoregulatory mechanisms. The small chromosome ParBs were found to silence transcription, a property relevant to autoregulation. PMID:16452432

  14. Epidemiological analyses on animal parasitoses: recent activity of the I.M.I.P.P.V.

    Science.gov (United States)

    Battelli, G; Capelli, G; Martini, M; Poglayen, G; Restani, R; Roda, R

    1989-01-01

    The paper presents a selected review of the epidemiological analyses performed in recent years (1984-1988) in the Institute of Malattie Infettive, Profilassi e Polizia Veterinaria of the University of Bologna on animal parasitoses, with special reference to the evaluation of some risk factors of bovine, swine and canine helminthoses, and to the validity of the coprological test for some parasites of the red fox.

  15. Recombination in the human Pseudoautosomal region PAR1.

    Directory of Open Access Journals (Sweden)

    Anjali G Hinch

    2014-07-01

    Full Text Available The pseudoautosomal region (PAR is a short region of homology between the mammalian X and Y chromosomes, which has undergone rapid evolution. A crossover in the PAR is essential for the proper disjunction of X and Y chromosomes in male meiosis, and PAR deletion results in male sterility. This leads the human PAR with the obligatory crossover, PAR1, to having an exceptionally high male crossover rate, which is 17-fold higher than the genome-wide average. However, the mechanism by which this obligatory crossover occurs remains unknown, as does the fine-scale positioning of crossovers across this region. Recent research in mice has suggested that crossovers in PAR may be mediated independently of the protein PRDM9, which localises virtually all crossovers in the autosomes. To investigate recombination in this region, we construct the most fine-scale genetic map containing directly observed crossovers to date using African-American pedigrees. We leverage recombination rates inferred from the breakdown of linkage disequilibrium in human populations and investigate the signatures of DNA evolution due to recombination. Further, we identify direct PRDM9 binding sites using ChIP-seq in human cells. Using these independent lines of evidence, we show that, in contrast with mouse, PRDM9 does localise peaks of recombination in the human PAR1. We find that recombination is a far more rapid and intense driver of sequence evolution in PAR1 than it is on the autosomes. We also show that PAR1 hotspot activities differ significantly among human populations. Finally, we find evidence that PAR1 hotspot positions have changed between human and chimpanzee, with no evidence of sharing among the hottest hotspots. We anticipate that the genetic maps built and validated in this work will aid research on this vital and fascinating region of the genome.

  16. Expression of protease-activated receptors 1 and 2 in individuals with healthy gingiva and chronic periodontitis

    Directory of Open Access Journals (Sweden)

    Sivasankari Thilagar

    2018-01-01

    Full Text Available Background: Protease-activated receptors (PAR-1 and PAR-2 are found to be immensely exhibited in the periodontal tissues. These receptors are involved in the inflammatory and repair processes. Activation of PARs is mainly by the bacterial and endogenous enzymes. The aim of the study was to determine the role of PAR-1 and PAR-2 in initiating periodontal inflammation and to immunolocalize these receptors in the gingival tissues of healthy and chronic periodontitis individuals. Materials and Methods: A total of 50 patients were selected for this study, of which 25 were healthy controls and 25 were chronic periodontitis patients. Gingival tissues were excised from the marginal gingiva and interdental papilla under local anesthesia (xylocaine with 2% adrenaline during crown lengthening procedure or during periodontal therapy depending on the respective groups. Immunohistochemical analyses of PARs were done by staining the samples with hematoxylin and eosin and with primary and secondary antibody for PAR-1 and PAR-2. Results: The Hematoxylin and Eosin staining showed more inflammatory changes in the periodontitis group compared to healthy gingiva. In chronic periodontitis, PAR-1-positive cells were seen in the basal layer with a weak expression and were showing negative expression in the superficial layer. In consideration of PAR-2, there was a very strong expression up to the superficial layer of the epithelium, compared to PAR-1. On comparing the intensity of staining in the connective tissue of chronic periodontitis sample, there was an increased expression of PAR-2 compared with PAR-1. A low positive expression of PAR-1 and PAR-2 was observed in the epithelium and connective tissue of the healthy tissues. Conclusion: The results clearly demonstrated the role of PAR-1 and 2 in periodontal inflammation.

  17. The Neural Bases of Difficult Speech Comprehension and Speech Production: Two Activation Likelihood Estimation (ALE) Meta-Analyses

    Science.gov (United States)

    Adank, Patti

    2012-01-01

    The role of speech production mechanisms in difficult speech comprehension is the subject of on-going debate in speech science. Two Activation Likelihood Estimation (ALE) analyses were conducted on neuroimaging studies investigating difficult speech comprehension or speech production. Meta-analysis 1 included 10 studies contrasting comprehension…

  18. How effective are active videogames among the young and the old? Adding meta-analyses to two recent systematic reviews

    NARCIS (Netherlands)

    Riet, J.P. van 't; Crutzen, R.M.M.; Lu, A.S.

    2014-01-01

    Objective: Two recent systematic reviews have surveyed the existing evidence for the effectiveness of active videogames in children/adolescents and in elderly people. In the present study, effect sizes were added to these systematic reviews, and meta-analyses were performed. Materials and Methods:

  19. Activity Based Learning in a Freshman Global Business Course: Analyses of Preferences and Demographic Differences

    Science.gov (United States)

    Levine, Mark F.; Guy, Paul W.

    2007-01-01

    The present study investigates pre-business students' reaction to Activity Based Learning in a lower division core required course entitled Introduction to Global Business in the business curriculum at California State University Chico. The study investigates students' preference for Activity Based Learning in comparison to a more traditional…

  20. Instrumental neutron and photon activation analyses of selected geochemical reference materials

    Czech Academy of Sciences Publication Activity Database

    Mizera, Jiří; Řanda, Zdeněk

    2010-01-01

    Roč. 284, č. 1 (2010), s. 157-163 ISSN 0236-5731 R&D Projects: GA AV ČR IAA300130706 Institutional research plan: CEZ:AV0Z10480505 Keywords : neutron activation analysis * photon activation analysis * geochemical reference materials Subject RIV: BG - Nuclear, Atomic and Molecular Physics, Colliders Impact factor: 0.777, year: 2010

  1. Instrumental neutron and photon activation analyses of selected geochemical reference materials

    Czech Academy of Sciences Publication Activity Database

    Mizera, Jiří; Řanda, Z.

    2010-01-01

    Roč. 284, č. 1 (2010), s. 157-163 ISSN 0236-5731 Institutional research plan: CEZ:AV0Z30460519 Keywords : neutron activation analysis * photon activation analysis * geochemical reference materials Subject RIV: BG - Nuclear, Atomic and Molecular Physics, Colliders OBOR OECD: Analytical chemistry Impact factor: 0.777, year: 2010

  2. A Relationship Between the Solar Rotation and Activity Analysed by Tracing Sunspot Groups

    Science.gov (United States)

    Ruždjak, Domagoj; Brajša, Roman; Sudar, Davor; Skokić, Ivica; Poljančić Beljan, Ivana

    2017-12-01

    The sunspot position published in the data bases of the Greenwich Photoheliographic Results (GPR), the US Air Force Solar Optical Observing Network and National Oceanic and Atmospheric Administration (USAF/NOAA), and of the Debrecen Photoheliographic Data (DPD) in the period 1874 to 2016 were used to calculate yearly values of the solar differential-rotation parameters A and B. These differential-rotation parameters were compared with the solar-activity level. We found that the Sun rotates more differentially at the minimum than at the maximum of activity during the epoch 1977 - 2016. An inverse correlation between equatorial rotation and solar activity was found using the recently revised sunspot number. The secular decrease of the equatorial rotation rate that accompanies the increase in activity stopped in the last part of the twentieth century. It was noted that when a significant peak in equatorial rotation velocity is observed during activity minimum, the next maximum is weaker than the previous one.

  3. The double par locus of virulence factor pB171: DNA segregation is correlated with oscillation of ParA

    DEFF Research Database (Denmark)

    Ebersbach, G; Gerdes, K; Charbon, Gitte Ebersbach

    2001-01-01

    Prokaryotic plasmids and chromosomes encode partitioning (par) loci that segregate DNA to daughter cells before cell division. Recent database analyses showed that almost all known par loci encode an ATPase and a DNA-binding protein, and one or more cis-acting regions where the proteins act. All...

  4. Analyses of the activation of near term fusion reactor compound materials

    International Nuclear Information System (INIS)

    Lengar, I.

    2007-01-01

    One of the important questions that still have to be solved for the next generation fusion reactors is the choice of the material to be used for the first wall. An important criteria is low activation due to neutron bombardment from the plasma. One of the promising materials is the SiC/SiC composite. Its main elemental constituents, namely the C and Si, have very good activation characteristics. The main contribution to activity arises, however, from trace elements, which are needed in the sintering process and remain in the material afterwards. Before the preparation process of the material, the activation characteristics of individual constituents are needed. The activation properties of the whole sample could than be estimated by summing the weighted properties of individual constituents. The activity of a particular trace element is, however, not necessarily dependent only on the percentage of the element in the sample, but also on the presence of other elements in the compound due to the charge particle production and/or (n, 2n) reactions. The extension of this effect is investigated and to what extent individual calculations, performed for a single element, mimic the real situation. Further the activation characteristic for several possible sintering aid elements is theoretically investigated with the use of the FISPACT inventory code. (author)

  5. Analysing the influence of human activity on runoff in the Weihe River basin

    Directory of Open Access Journals (Sweden)

    C. Shen

    2015-05-01

    Full Text Available Changing runoff patterns can have profound effects on the economic development of river basins. To assess the impact of human activity on runoff in the Weihe River basin, principal component analysis (PCA was applied to a set of 17 widely used indicators of economic development to construct general combined indicators reflecting different types of human activity. Grey relational analysis suggested that the combined indicator associated with agricultural activity was most likely to have influenced the changes in runoff observed within the river basin during 1994–2011. Curve fitting was then performed to characterize the relationship between the general agricultural indicator and the measured runoff, revealing a reasonably high correlation (R2 = 0.393 and an exponential relationship. Finally, a sensitivity analysis was performed to assess the influence of the 17 individual indicators on the measured runoff, confirming that indicators associated with agricultural activity had profound effects whereas those associated with urbanization had relatively little impact.

  6. Screening of marine seaweeds for bioactive compound against fish pathogenic bacteria and active fraction analysed by gas chromatography– mass spectrometry

    Directory of Open Access Journals (Sweden)

    Rajasekar Thirunavukkarasu

    2014-05-01

    Full Text Available Objective: To isolate bioactive molecules from marine seaweeds and check the antimicrobial activity against the fish pathogenic bacteria. Methods: Fresh marine seaweeds Gracilaria edulis, Kappaphycus spicifera, Sargassum wightii (S. wightii were collected. Each seaweed was extracted with different solvents. In the study, test pathogens were collected from microbial type culture collection. Antibacterial activity was carried out by using disc diffusion method and minimum inhibition concentration (MIC was calculated. Best seaweed was analysed by fourier transform infrared spectroscopy. The cured extract was separated by thin layer chromatography (TLC. Fraction was collected from TLC to check the antimicrobial activity. Best fraction was analysed by gas chromatography mass spectrometer (GCMS. Results: Based on the disc diffusion method, S. wightii showed a better antimicrobial activity than other seaweed extracts. Based on the MIC, methanol extract of S. wightii showed lower MIC than other solvents. S. wightii were separated by TLC. In this TLC, plate showed a two fraction. These two fractions were separated in preparative TLC and checked for their antimicrobial activity. Fraction 2 showed best MIC value against the tested pathogen. Fraction 2 was analysed by GCMS. Based on the GCMS, fraction 2 contains n-hexadecanoic acid (59.44%. Conclusions: From this present study, it can be concluded that S. wightii was potential sources of bioactive compounds.

  7. Flavonoid Analyses and Antimicrobial Activity of Various Parts of Phaleria macrocarpa (Scheff. Boerl Fruit

    Directory of Open Access Journals (Sweden)

    Ehsan Oskoueian

    2011-05-01

    Full Text Available Phaleria macrocarpa (Scheff. Boerl (Thymelaceae is commonly known as ‘Crown of God’, ‘Mahkota Dewa’, and ‘Pau’. It originates from Papua Island, Indonesia and it grows in tropical areas. Empirically, it is potent in treating the hypertensive,diabetic, cancer and diuretic patients. It has a long history of ethnopharmacological usage, and the lack of information about its biological activities led us to investigate the possible biological activities by characterisation of flavonoids and antimicrobial activity of various part of P. macrocarpa against pathogenic bacteria and fungi. The results showed that kaempferol, myricetin, naringin, and rutin were the major flavonoids present in the pericarp while naringin and quercetin were found in the mesocarp and seed. Furthermore, the antibacterial activity of different parts of P. macrocarpa fruit showed a weak ability to moderate antibacterial activity against pathogenic tested bacteria (inhibition range: 0.93–2.17 cm at concentration of 0.3 mg/disc. The anti fungi activity was only found in seed extract against Aspergillus niger (1.87 cm at concentration of 0.3 mg/well. From the results obtained, P. macrocarpa fruit could be considered as a natural antimicrobial source due to the presence of flavonoid compounds.

  8. Analyse des structures planaires multicouches à ferrite par la ...

    African Journals Online (AJOL)

    Finite element analysis of multi-layer planar structures with Ferrite. Electromagnetic Analysis of microwave integrated circuits is an attractive subject do to the recent development and use of new materials such as magnetic anisotropic materials (hexaferrite, ...). These materials are used in many microwave components ...

  9. Reading Actively Online: An Exploratory Investigation of Online Annotation Tools for Inquiry Learning / La lecture active en ligne: étude exploratoire sur les outils d'annotation en ligne pour l'apprentissage par l’enquête

    Directory of Open Access Journals (Sweden)

    Jingyan Lu

    2012-11-01

    Full Text Available This study seeks to design and facilitate active reading among secondary school students with an online annotation tool – Diigo. Two classes of different academic performance levels were recruited to examine their annotation behavior and perceptions of Diigo. We wanted to determine whether the two classes differed in how they used Diigo; how they perceived Diigo; and whether how they used Diigo was related to how they perceived it. Using annotation data and surveys in which students reported on their use and perceptions of Diigo, we found that although the tool facilitated individual annotations, the two classes used and perceived it differently. Overall, the study showed Diigo to be a promising tool for enhancing active reading in the inquiry learning process. Cette étude vise à concevoir et à faciliter la lecture active chez les élèves du secondaire grâce à l’outil d'annotation en ligne Diigo. Deux classes avec des niveaux de rendement scolaire différents ont été retenues afin qu’on examine leur manière d’annoter et leur perception de Diigo. Nous avons voulu déterminer si les deux classes diffèrent dans leur façon d’utiliser Diigo, leur perception de Diigo, et si leur manière d’utiliser Diigo était liée à leur perception. En utilisant les données d'annotation et d'enquêtes dans lesquelles les élèves relataient leur utilisation et leur perception de Diigo, nous avons constaté que, même si l'outil a facilité les annotations individuelles, les deux classes l’ont utilisé et perçu différemment. Dans l'ensemble, l'étude a montré que Diigo est un outil prometteur pour l'amélioration de la lecture active dans le processus d'apprentissage par enquête.

  10. Elusloom lennukiga puhkusele / Inge Parring

    Index Scriptorium Estoniae

    Parring, Inge

    2003-01-01

    Ilmunud ka: Delovõje Vedomosti 1. okt. lk. 13. Air Cargo Estonia/ACE Logisticsi müügijuht Inge Parring tutvustab elusloomade transpordivõimalusi. Vt. samas: Loomade transportimiseks vajalikud dokumendid

  11. Spectroscopic study of {sup 206,207,208}Pb isotopes by high resolution analysis of 24.5 MeV proton scattering; Etude spectroscopique des isotopes 206, 207 et 208 du plomb par analyse a haute resolution de la diffusion de protons de 24,5 MeV

    Energy Technology Data Exchange (ETDEWEB)

    Vallois, G [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

    1968-03-01

    {sup 206,207,208}pb have been studied by 24.5 MeV proton inelastic scattering with a resolution of 20 keV. The angular distributions of the differential cross-sections corresponding to the different excited levels have been measured in a large angular region and analysed with the DWBA.This work shows that it exists between 4 and 5 MeV of excitation energy some strongly excited levels corresponding to transfer momenta l = 2, 4, 6 and 8. The single particle-hole models do not explain these states; so it will probably be necessary to introduce some several particle - hole configurations. (author) [French] Les isotopes 206, 207 et 208 du plomb ont ete etudies par diffusion inelastique de protons de 24,5 MeV avec une resolution de 20 keV. Les distributions angulaires des sections efficaces differentielles correspondant aux differents niveaux excites ont ete mesurees sur un large domaine angulaire et analysees a l'aide de la DWBA. Ce travail met en evidence l'existence, entre 4 et 5 MeV d'excitation, de niveaux fortement excites correspondant a des moments de transfert de 2, 4, 6 et 8. Les modeles a simple particule-trou ne rendant pas compte de ces niveaux, il faudra sans doute recourir a des configurations a plusieurs particules-trous pour les expliquer. (auteur)

  12. Par Pond vegetation status 1996

    International Nuclear Information System (INIS)

    Mackey, H.E. Jr.; Riley, R.S.

    1996-12-01

    The water level of Par Pond was lowered approximately 20 feet in mid-1991 in order to protect downstream residents from possible dam failure suggested by subsidence on the downstream slope of the dam and to repair the dam. This lowering exposed both emergent and nonemergent macrophyte beds to drying conditions resulting in extensive losses. A survey of the newly emergent, shoreline aquatic plant communities of Par Pond began in June 1995, three months after the refilling of Par Pond to approximately 200 feet above mean sea level. These surveys continued in July, September, and late October, 1995, and into the early spring and late summer of 1996. Communities similar to the pre-drawdown, Par Pond aquatic plant communities continue to become re-established. Emergent beds of maidencane, lotus, waterlily, watershield, and Pontederia are extensive and well developed. Measures of percent cover, width of beds, and estimates of area of coverage with satellite data indicate regrowth within two years of from 40 to 60% of levels prior to the draw down. Cattail occurrence continued to increase during the summer of 1996, especially in the former warm arm of Par Pond, but large beds common to Par Pond prior to the draw down still have not formed. Lotus has invaded and occupies many of the areas formerly dominated by cattail beds. To track the continued development of macrophytes in Par Pond, future surveys through the summer and early fall of 1997, along with the evaluation of satellite data to map the extent of the macrophyte beds of Par Pond, are planned

  13. First (18)F-labeled ligand for PET imaging of uPAR

    DEFF Research Database (Denmark)

    Persson, Morten; Liu, Hongguang; Madsen, Jacob

    2013-01-01

    Urokinase-type plasminogen activator receptor (uPAR) is overexpressed in human prostate cancer and uPAR has been found to be associated with metastatic disease and poor prognosis. AE105 is a small linear peptide with high binding affinity to uPAR. We synthesized an N-terminal NOTA......-conjugated version (NOTA-AE105) for development of the first (18)F-labeled uPAR positron-emission-tomography PET ligand using the Al(18)F radiolabeling method. In this study, the potential of (18)F-AlF-NOTA-AE105 to specifically target uPAR-positive prostate tumors was investigated....

  14. Multilevel Analyses of School and Children's Characteristics Associated with Physical Activity

    Science.gov (United States)

    Gomes, Thayse Natacha; dos Santos, Fernanda K.; Zhu, Weimo; Eisenmann, Joey; Maia, José A. R.

    2014-01-01

    Background: Children spend most of their awake time at school, and it is important to identify individual and school-level correlates of their physical activity (PA) levels. This study aimed to identify the between-school variability in Portuguese children PA and to investigate student and school PA correlates using multilevel modeling. Methods:…

  15. Detailed Structural Analyses of KOH Activated Carbon from Waste Coffee Beans

    Science.gov (United States)

    Takahata, Tomokazu; Toda, Ikumi; Ono, Hiroki; Ohshio, Shigeo; Akasaka, Hiroki; Himeno, Syuji; Kokubu, Toshinori; Saitoh, Hidetoshi

    2009-11-01

    The relationship of the detailed structural change of KOH activated carbon and hydrogen storage ability was investigated in activated carbon materials fabricated from waste coffee beans. The specific surface area of porous carbon materials calculated from N2 adsorption isotherms stood at 2070 m2/g when the weight ratio of KOH to carbon materials was 5:1, and pore size was in the range of approximately 0.6 to 1.1 nm as micropores. In the structural analysis, X-ray diffraction analysis and Raman spectroscopy indicated structural change in these carbon materials through KOH activation. The order of the graphite structure changed to a smaller scale with this activation. It is theorized that specific surface area increased using micropores provided by carbon materials developed from the descent of the graphite structure. Hydrogen storage ability improved with these structural changes, and reached 0.6 wt % at 2070 m2/g. These results suggest that hydrogen storage ability is conferred by the chemical effect on graphite of carbon materials.

  16. Interactive Whiteboards and All That Jazz: Analysing Classroom Activity with Interactive Technologies

    Science.gov (United States)

    Tanner, Howard; Beauchamp, Gary; Jones, Sonia; Kennewell, Steve

    2010-01-01

    The term "orchestration", has been used to describe the teacher's role in activity settings incorporating interactive technologies. This musical analogy suggests pre-planned manipulation of events to generate "performance" leading to learning. However, in two recent projects we have observed how effective teaching and learning…

  17. Hidden entrepreneurship: Multilevel analyses of the determinants and consequences of entrepreneurial employee activity

    NARCIS (Netherlands)

    Liebregts, W.J.

    2018-01-01

    This dissertation aims to contribute to the fast-growing field of entrepreneurship research. With entrepreneurship one usually refers to activities by individuals running a business for own risk and reward (Jensen & Meckling, 1976; Knight, 1921). This, however, neglects the discovery, evaluation and

  18. MEG time-frequency analyses for pre- and post-surgical evaluation of patients with epileptic rhythmic fast activity.

    Science.gov (United States)

    Sueda, Keitaro; Takeuchi, Fumiya; Shiraishi, Hideaki; Nakane, Shingo; Asahina, Naoko; Kohsaka, Shinobu; Nakama, Hideyuki; Otsuki, Taisuke; Sawamura, Yutaka; Saitoh, Shinji

    2010-02-01

    To evaluate the effectiveness of surgery for epilepsy, we analyzed rhythmic fast activity by magnetoencephalography (MEG) before and after surgery using time-frequency analysis. To assess reliability, the results obtained by pre-surgical MEG and intraoperative electrocorticography were compared. Four children with symptomatic localization-related epilepsy caused by circumscribed cortical lesion were examined in the present study using 204-channel helmet-shaped MEG with a sampling rate of 600Hz. One patient had dysembryoplastic neuroepithelial tumor (DNT) and three patients had focal cortical dysplasia (FCD). Aberrant areas were superimposed, to reconstruct 3D MRI images, and illustrated as moving images. In three patients, short-time Fourier transform (STFT) analyses of MEG showed rhythmic activities just above the lesion with FCD and in the vicinity of DNT. In one patient with FCD in the medial temporal lobe, rhythmic activity appeared in the ipsilateral frontal lobe and temporal lateral aspect. These findings correlate well with the results obtained by intraoperative electrocorticography. After the surgery, three patients were relieved of their seizures, and the area of rhythmic MEG activity disappeared or become smaller. One patient had residual rhythmic MEG activity, and she suffered from seizure relapse. Time-frequency analyses using STFT successfully depicted MEG rhythmic fast activity, and would provide valuable information for pre- and post-surgical evaluations to define surgical strategies for patients with epilepsy.

  19. Determination of trace elements in BCR single cell protein via destructive neutron activation analyses

    International Nuclear Information System (INIS)

    Tjioe, P.S.; Goeij, J.J.M. de; Nooijen, J.L.; Kroon, J.J.

    1978-10-01

    The amount of some trace elements in single cell protein (SCP), a product of BP Research Centre at Sunbury-at-Thames, England, was determined by neutron activation analysis. The SCP-samples were irradiated in the reactor of the Interuniversity Reactor Institute at Delft in a neutron flux of 1.0x10 13 n/cm 2 s for 12 hours. Samples of Bowen's Kale were used as reference material. After a decay of two or three days the samples were chemically destroyed, and the trace elements were separated. The quantity of the following elements was determined by measuring the γ-activity by means of a scintillation counter: antimony, cadmium, mercury, arsenic and selenium. The amounts of these elements in the SCP and in the reference material were tabled

  20. Qualitative Elemental Analyses of a Meteorite Sample Found in Turkey by Photo-activation Analysis Method

    International Nuclear Information System (INIS)

    Ertugay, C; Boztosun, I; Ozmen, S F; Dapo, H

    2015-01-01

    In this paper, a meteorite sample provided from TÜBITAK National Observatory found in Turkey has been investigated by using a clinical linear accelerator that has endpoint energy of 18 MeV, and a high purity Germanium detector for qualitative elemental analysis within photo-activation analysis method. 21 nuclei ranging from 24Na to 149Nd have been identified in the meteorite sample. (paper)

  1. Phenotype specific analyses reveal distinct regulatory mechanism for chronically activated p53.

    Directory of Open Access Journals (Sweden)

    Kristina Kirschner

    2015-03-01

    Full Text Available The downstream functions of the DNA binding tumor suppressor p53 vary depending on the cellular context, and persistent p53 activation has recently been implicated in tumor suppression and senescence. However, genome-wide information about p53-target gene regulation has been derived mostly from acute genotoxic conditions. Using ChIP-seq and expression data, we have found distinct p53 binding profiles between acutely activated (through DNA damage and chronically activated (in senescent or pro-apoptotic conditions p53. Compared to the classical 'acute' p53 binding profile, 'chronic' p53 peaks were closely associated with CpG-islands. Furthermore, the chronic CpG-island binding of p53 conferred distinct expression patterns between senescent and pro-apoptotic conditions. Using the p53 targets seen in the chronic conditions together with external high-throughput datasets, we have built p53 networks that revealed extensive self-regulatory 'p53 hubs' where p53 and many p53 targets can physically interact with each other. Integrating these results with public clinical datasets identified the cancer-associated lipogenic enzyme, SCD, which we found to be directly repressed by p53 through the CpG-island promoter, providing a mechanistic link between p53 and the 'lipogenic phenotype', a hallmark of cancer. Our data reveal distinct phenotype associations of chronic p53 targets that underlie specific gene regulatory mechanisms.

  2. Round-robin activities on finite element analyses of elastic-plastic fracture in Japan

    International Nuclear Information System (INIS)

    Shimakawa, T.; Takahashi, Y.; Yagawa, G.

    1989-01-01

    The establishment of the leak-before-break (LBB) concept requires a method to evaluate the fracture characteristics. The finite element method can be used for this purpose but the solution is more or less influenced by the method employed. In this study, two round-robin analyses are performed for three-dimensional crack problems. The first problem is for surface crack growth in a carbon steel plate subjected to tension loading. Ten solutions are obtained by ten participants, and calculated results are compared with each other as to the applied load, displacement and J-integral. Though the relation between applied load and displacement is affected by modeling of the stress-strain curve, fairly good agreement is obtained between the solutions. The second problem is for a circumferential part-through crack in a carbon steel pipe subjected to a bending moment. Nine solutions are obtained by eight participants. The difference between the solutions is relatively significant as to the relation between J-integral and load-point displacement. A discussion is made about the sources of difference between each solution. (orig.)

  3. Results of de-novo and Motif activity analyses - FANTOM5 | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available switchLanguage; BLAST Search Image Search Home About Archive Update History Data List Contact us FANTOM... JASPAR) Data file File name: Motifs File URL: ftp://ftp.biosciencedbc.jp/archive/fantom5/datafiles/phase1.3...tabase Database Description Download License Update History of This Database Site Policy | Contact Us Results of de-novo and Motif activity analyses - FANTOM5 | LSDB Archive ...

  4. Velocity Analysis Using Nonhyperbolic Move-Out in Anisotropic Media of Arbitrary Symmetry: Synthetic and Field Data Studies Analyse de vitesse par correction non hyperbolique des indicatrices dans les milieux anisotropes de symétrie arbitraire : étude sur des données synthétiques et réelles

    Directory of Open Access Journals (Sweden)

    Tabti H.

    2006-12-01

    une analyse de cohérence le long d'indicatrices non hyperboliques (i. e. comprenant les longs déports source-récepteur permettant d'évaluer les paramètres effectifs suivis d'un processus de Dix pour l'estimation des paramètres d'intervalle. D'autre part, Sayers et Ebrom (1997 ont proposé de leur côté une autre expression non hyperbolique du temps de trajet ainsi que les expressions de Dix correspondantes valables pour les milieux tabulaires présentant des anisotropies azimutales. La méthode a été testée sur des modèles synthétiques homogènes présentant des anisotropies variées. Nous proposons ici une généralisation de la méthode présentée par Alkhalifah, applicable à des milieux tabulaires d'anisotropie quelconque mais modérée (i. e. avec des degrés d'anisotropie pouvant aller jusqu'à environ 20 %. La paramétrisation de la vitesse est une extension naturelle de celle utilisée par l'auteur précédent. Elle s'exprime à l'aide des paramètres généralisés de Thomsen (1986 développés par Mensch et Rasolofosaon (1997. Dans un premier temps, la méthode est appliquée sur des données synthétiques obtenues à partir d'un modèle à 6 couches présentant des anisotropies variées (à la fois le type et le degré. La robustesse de la méthode y est démontrée. En effet, tous les paramètres d'intervalle (ici Vnmo et la vitesse horizontale Vh sont estimés de façon satisfaisante (erreur < 2 %, à comparer aux degrés d'anisotropie qui varient de 15 à 20 % et ce pour tous les azimuts. La méthode est ensuite appliquée sur des données réelles de mer du Nord comprenant trois profils sismiques se croisant au niveau d'un puits.

  5. In vitro activity of five quinolones and analysis of the quinolone resistance-determining regions of gyrA, gyrB, parC, and parE in Ureaplasma parvum and Ureaplasma urealyticum clinical isolates from perinatal patients in Japan.

    Science.gov (United States)

    Kawai, Yasuhiro; Nakura, Yukiko; Wakimoto, Tetsu; Nomiyama, Makoto; Tokuda, Tsugumichi; Takayanagi, Toshimitsu; Shiraishi, Jun; Wasada, Kenshi; Kitajima, Hiroyuki; Fujita, Tomio; Nakayama, Masahiro; Mitsuda, Nobuaki; Nakanishi, Isao; Takeuchi, Makoto; Yanagihara, Itaru

    2015-04-01

    Ureaplasma spp. cause several disorders, such as nongonococcal urethritis, miscarriage, and preterm delivery with lung infections in neonates, characterized by pathological chorioamnionitis in the placenta. Although reports on antibiotic resistance in Ureaplasma are on the rise, reports on quinolone-resistant Ureaplasma infections in Japan are limited. The purpose of this study was to determine susceptibilities to five quinolones of Ureaplasma urealyticum and Ureaplasma parvum isolated from perinatal samples in Japan and to characterize the quinolone resistance-determining regions in the gyrA, gyrB, parC, and parE genes. Out of 28 clinical Ureaplasma strains, we isolated 9 with high MICs of quinolones and found a single parC gene mutation, resulting in the change S83L. Among 158 samples, the ParC S83L mutation was found in 37 samples (23.4%), including 1 sample harboring a ParC S83L-GyrB P462S double mutant. Novel mutations of ureaplasmal ParC (S83W and S84P) were independently found in one of the samples. Homology modeling of the ParC S83W mutant suggested steric hindrance of the quinolone-binding pocket (QBP), and de novo prediction of peptide structures revealed that the ParC S84P may break/kink the formation of the α4 helix in the QBP. Further investigations are required to unravel the extent and mechanism of antibiotic resistance of Ureaplasma spp. in Japan. Copyright © 2015, American Society for Microbiology. All Rights Reserved.

  6. Determination of trace elements in bottled water in Greece by instrumental and radiochemical neutron activation analyses

    International Nuclear Information System (INIS)

    Soupioni, M.J.; Symeopoulos, B.D.; Papaefthymiou, H.V.

    2006-01-01

    Four different bottled water brands sold in Greece in the winter of 2001-2002 were analyzed for a wide range of chemical elements, using neutron activation analysis (NAA). The elements Na and Br were determined instrumentally (INAA), whereas the other metals and trace elements radiochemically (RNAA). The results indicated that the mean level of all the elements determined in the samples were well within the European Union (EU) directive on drinking water and accomplish the drinking water standards of the World Health Organisation (WHO) as well as of the Food and Drug Administration (FDA). (author)

  7. Locomotor activity during the frenzy swim: analysing early swimming behaviour in hatchling sea turtles.

    Science.gov (United States)

    Pereira, Carla M; Booth, David T; Limpus, Colin J

    2011-12-01

    Swimming effort of hatchling sea turtles varies across species. In this study we analysed how swim thrust is produced in terms of power stroke rate, mean maximum thrust per power stroke and percentage of time spent power stroking throughout the first 18 h of swimming after entering the water, in both loggerhead and flatback turtle hatchlings and compared this with previous data from green turtle hatchlings. Loggerhead and green turtle hatchlings had similar power stroke rates and percentage of time spent power stroking throughout the trial, although mean maximum thrust was always significantly higher in green hatchlings, making them the most vigorous swimmers in our three-species comparison. Flatback hatchlings, however, were different from the other two species, with overall lower values in all three swimming variables. Their swimming effort dropped significantly during the first 2 h and kept decreasing significantly until the end of the trial at 18 h. These results support the hypothesis that ecological factors mould the swimming behaviour of hatchling sea turtles, with predator pressure being important in determining the strategy used to swim offshore. Loggerhead and green turtle hatchlings seem to adopt an intensely vigorous and energetically costly frenzy swim that would quickly take them offshore into the open ocean in order to reduce their exposure to near-shore aquatic predators. Flatback hatchlings, however, are restricted in geographic distribution and remain within the continental shelf region where predator pressure is probably relatively constant. For this reason, flatback hatchlings might use only part of their energy reserves during a less vigorous frenzy phase, with lower overall energy expenditure during the first day compared with loggerhead and green turtle hatchlings.

  8. Ab initio computational study of vincristine as a biological active compound: NMR and NBO analyses

    Directory of Open Access Journals (Sweden)

    Shiva Joohari

    2015-06-01

    Full Text Available Vincristine is a biological active alkaloid that has been used clinically against a variety of neoplasms. In the current study we have theoretically investigated the magnetic properties of titled compound to predict physical and chemical properties of vincristine as a biological inhibitor. Ab initio computation using HF and B3LYP with 3-21G(d and 6-31G(d level of theory have been performed and then magnetic shielding tensor (, ppm, shielding asymmetry (, magnetic shielding anisotropy (aniso, ppm, the skew of a tensor (K, chemical shift anisotropy ( and chemical shift ( were calculated to indicate the details of the interaction mechanism between microtubules and vincristine. Moreover, EHOMO, ELUMO and Ebg were evaluated. The maximum and minimum values of Ebg were found in HF/3-21g and B3LYP/3-21g respectively. It was also uggested that O24, O37, O49 and O55 with minimum values of iso, are active sites of titled compound. Furthermore the calculated chemical shifts were compared with experimental data in DMSO and CDCl3 solvents.

  9. Lysosomal acid lipase deficiency in rats: Lipid analyses and lipase activities in liver and spleen

    International Nuclear Information System (INIS)

    Kuriyama, M.; Yoshida, H.; Suzuki, M.; Fujiyama, J.; Igata, A.

    1990-01-01

    We report the biological characterization of an animal model of a genetic lipid storage disease analogous to human Wolman's disease. Affected rats accumulated cholesteryl esters (13.3-fold), free cholesterol (2.8-fold), and triglycerides (5.4-fold) in the liver, as well as cholesteryl esters (2.5-fold) and free cholesterol (1.33-fold) in the spleen. Triglycerides did not accumulate, and the levels actually decreased in the spleen. Analysis of the fatty acid composition of the cholesteryl esters and triglycerides showed high percentages of linoleic acid (18:2) and arachidonic acid (20:4) in both organs, especially in the liver. No accumulation of phospholipids, neutral glycosphingolipids, or gangliosides was found in the affected rats. Acid lipase activity for [14C]triolein, [14C]cholesteryl oleate, and 4-methyl-umbelliferyl oleate was deficient in both the liver and spleen of affected rats. Lipase activity at neutral pH was normal in both liver and spleen. Heterozygous rats showed intermediate utilization of these substrates in both organs at levels between those for affected rats and those for normal controls, although they did not accumulate any lipids. These data suggest that these rats represent an animal counterpart of Wolman's disease in humans

  10. A wavenumber approach to analysing the active control of plane waves with arrays of secondary sources

    Science.gov (United States)

    Elliott, Stephen J.; Cheer, Jordan; Bhan, Lam; Shi, Chuang; Gan, Woon-Seng

    2018-04-01

    The active control of an incident sound field with an array of secondary sources is a fundamental problem in active control. In this paper the optimal performance of an infinite array of secondary sources in controlling a plane incident sound wave is first considered in free space. An analytic solution for normal incidence plane waves is presented, indicating a clear cut-off frequency for good performance, when the separation distance between the uniformly-spaced sources is equal to a wavelength. The extent of the near field pressure close to the source array is also quantified, since this determines the positions of the error microphones in a practical arrangement. The theory is also extended to oblique incident waves. This result is then compared with numerical simulations of controlling the sound power radiated through an open aperture in a rigid wall, subject to an incident plane wave, using an array of secondary sources in the aperture. In this case the diffraction through the aperture becomes important when its size is compatible with the acoustic wavelength, in which case only a few sources are necessary for good control. When the size of the aperture is large compared to the wavelength, and diffraction is less important but more secondary sources need to be used for good control, the results then become similar to those for the free field problem with an infinite source array.

  11. Energy efficiency analyses of active flow aftertreatment systems for lean burn internal combustion engines

    International Nuclear Information System (INIS)

    Zheng Ming; Reader, Graham T.

    2004-01-01

    The use of three way catalytic converters in stoichiometric burn reciprocating internal combustion engine systems has proved to be an effective and efficient method for reducing the level of criteria pollutants. However, such passive systems have not been as successful in emission amelioration when combined with lean burn engines. This is because of the thermochemical nature of the exhaust gases generated by such engines. The high content of exhaust oxygen largely negates the effectiveness of three way catalytic converters, and the comparatively low temperature of the combusted gases means that supplemental energy has to be added to these gases to enable the converter to function correctly. This requirement severely reduces the energy efficiency of conventional passive aftertreatment systems. However, initial empirical studies have indicated that a possible means of improving the performance of aftertreatment devices when used with lean burn engine systems is to use active flow control of the exhaust gases. These results are reported in this paper. This concept has been further investigated by developing an energy efficiency analysis that enables the effects on aftertreatment performance of different gas flow rates, flow reversal frequencies and monolith solid properties to be investigated. Simulation results indicate that through active thermal management, the supplemental energy consumption can be drastically reduced

  12. Determination of correction coefficients for quantitative analysis by mass spectrometry. Application to uranium impurities analysis; Recherche des coefficients de correction permettant l'analyse quantitative par spectrometrie de masse. Application a l'analyse d'impuretes dans l'uranium

    Energy Technology Data Exchange (ETDEWEB)

    Billon, J P [Commissariat a l' Energie Atomique, Bruyeres-le-Chatel (France). Centre d' Etudes

    1970-07-01

    Some of basic principles in spark source mass spectrometry are recalled. It is shown how this method can lead to quantitative analysis when attention is paid to some theoretical aspects. A time constant relation being assumed between the analysed solid sample and the ionic beam it gives we determined experimental relative sensitivity factors for impurities in uranium matrix. Results being in fairly good agreement with: an unelaborate theory on ionization yield in spark-source use of theoretically obtained relative sensitivity factors in uranium matrix has been developed. (author) [French] Apres avoir rappele quelques principes fondamentaux regissant la spectrometrie de masse a etincelles, nous avons montre que moyennant un certain nombre de precautions, il etait possible d'utiliser cette methode en analyse quantitative. Ayant admis qu'il existait une relation constante dans le temps entre l'echantillon solide analyse et le faisceau ionique qui en est issu, nous avons d'abord entrepris de determiner des coefficients de correction experimentaux pour des matrices d'uranium. Les premiers resultats pratiques semblant en accord avec une theorie simple relative au rendement d'ionisation dans la source a etincelles, nous avons etudie la possibilite d'appliquer directement les coefficients theoriques ainsi definis, l'application etant toujours faite sur des matrices d'uranium. (auteur)

  13. General practitioner advice on physical activity: Analyses in a cohort of older primary health care patients (getABI

    Directory of Open Access Journals (Sweden)

    Thiem Ulrich

    2011-05-01

    Full Text Available Abstract Background Although the benefits of physical activity for health and functioning are recognized to extend throughout life, the physical activity level of most older people is insufficient with respect to current guidelines. The primary health care setting may offer an opportunity to influence and to support older people to become physically active on a regular basis. Currently, there is a lack of data concerning general practitioner (GP advice on physical activity in Germany. Therefore, the aim of this study was to evaluate the rate and characteristics of older patients receiving advice on physical activity from their GP. Methods This is a cross-sectional study using data collected at 7 years of follow-up of a prospective cohort study (German epidemiological trial on ankle brachial index, getABI. 6,880 unselected patients aged 65 years and above in the primary health care setting in Germany were followed up since October 2001. During the 7-year follow-up telephone interview, 1,937 patients were asked whether their GP had advised them to get regular physical activity within the preceding 12 months. The interview also included questions on socio-demographic and lifestyle variables, medical conditions, and physical activity. Logistic regression analysis (unadjusted and adjusted for all covariables was used to examine factors associated with receiving advice. Analyses comprised only complete cases with regard to the analysed variables. Results are expressed as odds ratios (ORs with 95% confidence intervals (95% CI. Results Of the 1,627 analysed patients (median age 77; range 72-93 years; 52.5% women, 534 (32.8% stated that they had been advised to get regular physical activity. In the adjusted model, those more likely to receive GP advice on physical activity were men (OR [95% CI] 1.34 [1.06-1.70], patients suffering from pain (1.43 [1.13-1.81], coronary heart disease and/or myocardial infarction (1.56 [1.21-2.01], diabetes mellitus (1

  14. Pretreatment procedures applied to samples to be analysed by neutron activation analysis at CDTN/CNEN

    International Nuclear Information System (INIS)

    Francisco, Dovenir; Menezes, Maria Angela de Barros Correia

    2009-01-01

    The neutron activation technique - using several methods - has been applied in 80% of the analytical demand of Division for Reactor and Analytical Techniques at CDTN/CNEN, Belo Horizonte, Minas Gerais. This scenario emphasizes the responsibility of the Laboratory to provide and assure the quality of the measurements. The first step to assure the results quality is the preparation of the samples. Therefore, this paper describes the experimental procedures adopted at CDTN/CNEN in order to uniform conditions of analysis and to avoid contaminations by elements present everywhere. Some of the procedures are based on methods described in the literature; others are based on many years of experience preparing samples from many kinds of matrices. The procedures described are related to geological material - soil, sediment, rock, gems, clay, archaeological ceramics and ore - biological materials - hair, fish, plants, food - water, etc. Analytical results in sediment samples are shown as n example pointing out the efficiency of the experimental procedure. (author)

  15. Metabolomics analyses identify platelet activating factors and heme breakdown products as Lassa fever biomarkers.

    Directory of Open Access Journals (Sweden)

    Trevor V Gale

    2017-09-01

    Full Text Available Lassa fever afflicts tens of thousands of people in West Africa annually. The rapid progression of patients from febrile illness to fulminant syndrome and death provides incentive for development of clinical prognostic markers that can guide case management. The small molecule profile of serum from febrile patients triaged to the Viral Hemorrhagic Fever Ward at Kenema Government Hospital in Sierra Leone was assessed using untargeted Ultra High Performance Liquid Chromatography Mass Spectrometry. Physiological dysregulation resulting from Lassa virus (LASV infection occurs at the small molecule level. Effects of LASV infection on pathways mediating blood coagulation, and lipid, amino acid, nucleic acid metabolism are manifest in changes in the levels of numerous metabolites in the circulation. Several compounds, including platelet activating factor (PAF, PAF-like molecules and products of heme breakdown emerged as candidates that may prove useful in diagnostic assays to inform better care of Lassa fever patients.

  16. Some analyses on the plasma motion in the space active region of the axial symmetry

    International Nuclear Information System (INIS)

    Li Zhongyuan; Hu Wenrui.

    1986-04-01

    In general, the potential magnetic field may gradually be twisted into the force-free magnetic field with the current produced by plasma rotation. In this paper, it is pointed out that if the magnetic field has no singularity on the symmetric axis, then the potential magnetic field cannot be twisted into the force-free magnetic field. Namely, it is not a perfect approach that the energy storage is only caused by the pure azimuthal motion in the active region. Besides the pure spiral motion, the unsteady coupling process between the magnetic field and both the toroidal and the poloidal velocity components should be analyzed. Finally, in the present note, some features of the kinematical force-free magnetic field of the axial symmetry are presented by the authors. (author)

  17. Neutron activation analyses and half-life measurements at the usgs triga reactor

    Science.gov (United States)

    Larson, Robert E.

    Neutron activation of materials followed by gamma spectroscopy using high-purity germanium detectors is an effective method for making measurements of nuclear beta decay half-lives and for detecting trace amounts of elements present in materials. This research explores applications of neutron activation analysis (NAA) in two parts. Part 1. High Precision Methods for Measuring Decay Half-Lives, Chapters 1 through 8 Part one develops research methods and data analysis techniques for making high precision measurements of nuclear beta decay half-lives. The change in the electron capture half-life of 51Cr in pure chromium versus chromium mixed in a gold lattice structure is explored, and the 97Ru electron capture decay half-life are compared for ruthenium in a pure crystal versus ruthenium in a rutile oxide state, RuO2. In addition, the beta-minus decay half-life of 71mZn is measured and compared with new high precision findings. Density Functional Theory is used to explain the measured magnitude of changes in electron capture half-life from changes in the surrounding lattice electron configuration. Part 2. Debris Collection Nuclear Diagnostic at the National Ignition Facility, Chapters 9 through 11 Part two explores the design and development of a solid debris collector for use as a diagnostic tool at the National Ignition Facility (NIF). NAA measurements are performed on NIF post-shot debris collected on witness plates in the NIF chamber. In this application NAA is used to detect and quantify the amount of trace amounts of gold from the hohlraum and germanium from the pellet present in the debris collected after a NIF shot. The design of a solid debris collector based on material x-ray ablation properties is given, and calculations are done to predict performance and results for the collection and measurements of trace amounts of gold and germanium from dissociated hohlraum debris.

  18. First-in-human uPAR PET

    DEFF Research Database (Denmark)

    Persson, Morten; Skovgaard, Dorthe; Brandt-Larsen, Malene

    2015-01-01

    A first-in-human clinical trial with Positron Emission Tomography (PET) imaging of the urokinase-type plasminogen activator receptor (uPAR) in patients with breast, prostate and bladder cancer, is described. uPAR is expressed in many types of human cancers and the expression is predictive...... for targeted molecular imaging with PET. The safety, pharmacokinetic, biodistribution profile and radiation dosimetry after a single intravenous dose of (64)Cu-DOTA-AE105 were assessed by serial PET and computed tomography (CT) in 4 prostate, 3 breast and 3 bladder cancer patients. Safety assessment...... of invasion, metastasis and indicates poor prognosis. uPAR PET imaging therefore holds promise to be a new and innovative method for improved cancer diagnosis, staging and individual risk stratification. The uPAR specific peptide AE105 was conjugated to the macrocyclic chelator DOTA and labeled with (64)Cu...

  19. Analyse quantitative détaillée des distillats moyens par couplage CG/SM. Application à l'étude des schémas réactionnels du procédé d'hydrotraitement Quantitative Analysis of Middle Distillats by Gc/Ms Coupling. Application to Hydrotreatment Process Mechanisms

    Directory of Open Access Journals (Sweden)

    Fafet A.

    2006-11-01

    Full Text Available L'analyse détaillée des distillats moyens est une étape indispensable à la compréhension des mécanismes réactionnels et à la cinétique de certains procédés de raffinage comme l'hydrotraitement. Une nouvelle méthode associant, d'une part un couplage chromatographie en phase gazeuse/spectrométrie de masse (CG/SM et, d'autre part une analyse quantitative par famille chimique par spectrométrie de masse a été développée. La chromatographie en phase gazeuse, réalisée sur une colonne apolaire, effectue la distillation des composés présents dans le gazole et la spectrométrie de masse quantifie les familles chimiques par intervalle de nombre d'atomes de carbone ou de point d'ébullition. Elle permet d'accéder ainsi à la répartition par nombre d'atomes de carbone de chaque famille chimique (alcanes, cycloalcanes, hydrocarbures aromatiques à un ou plusieurs noyaux, hydrocarbures aromatiques soufrés. Cette méthode a été validée et appliquée à une charge et à une recette d'hydrotraitement. A detailed analysis of middle distillates is essential for understanding the reaction mechanism and for studying the kinetics of refining processes such hydrotreatment. In fact, when we see the complexity of saturated and aromatic hydrocarbon mixtures appearing in gas oil, we realize that it's necessary to have a very detailed analysis of those cuts to understand the mechanisms involved in refining processes and to be able to describe their kinetics. Each gas oil has a very different composition and therefore a specific reactivity. That is why we have tried to develop predictive kinetic models to avoid experimenting in pilot plants, which is very expensive. But, even if all the compounds of a gasoline (PI-200°C have now been identified and quantified, using gas chromatography (1, such is not the case for heavier cuts. Only an overall characterization can be made, by chemical family. The techniques employed are, for example, HPLC (3,4 or

  20. Core microbial functional activities in ocean environments revealed by global metagenomic profiling analyses.

    Directory of Open Access Journals (Sweden)

    Ari J S Ferreira

    Full Text Available Metagenomics-based functional profiling analysis is an effective means of gaining deeper insight into the composition of marine microbial populations and developing a better understanding of the interplay between the functional genome content of microbial communities and abiotic factors. Here we present a comprehensive analysis of 24 datasets covering surface and depth-related environments at 11 sites around the world's oceans. The complete datasets comprises approximately 12 million sequences, totaling 5,358 Mb. Based on profiling patterns of Clusters of Orthologous Groups (COGs of proteins, a core set of reference photic and aphotic depth-related COGs, and a collection of COGs that are associated with extreme oxygen limitation were defined. Their inferred functions were utilized as indicators to characterize the distribution of light- and oxygen-related biological activities in marine environments. The results reveal that, while light level in the water column is a major determinant of phenotypic adaptation in marine microorganisms, oxygen concentration in the aphotic zone has a significant impact only in extremely hypoxic waters. Phylogenetic profiling of the reference photic/aphotic gene sets revealed a greater variety of source organisms in the aphotic zone, although the majority of individual photic and aphotic depth-related COGs are assigned to the same taxa across the different sites. This increase in phylogenetic and functional diversity of the core aphotic related COGs most probably reflects selection for the utilization of a broad range of alternate energy sources in the absence of light.

  1. Analyses of hemolymph from Amblyomma cajennense (Acari: ixodidae) using neutron activation analysis (NAA)

    Energy Technology Data Exchange (ETDEWEB)

    Simons, Simone M.; Oliveira, Daniella G.L.; Chudzinski-Tavassi, Ana M., E-mail: daniellaoliveira@butantan.gov.b, E-mail: amchudzinki@butantan.gov.b [Instituto Butantan, Sao Paulo, SP (Brazil); Zamboni, Cibele B., E-mail: czamboni@ipen.b [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil)

    2011-07-01

    Instrumental neutron activation analysis technique (INAA) was applied to determine the elemental composition of hemolymph from Amblyomma cajennense tick. This biological material came from Butantan Institute (Sao Paulo city, Brazil) and it was investigated using the IEA-R1 nuclear reactor (4MW, pool type) at IPEN/CNEN-SP - Brazil. The concentration values for: Br (0.0032 {+-} 0.0005gL{sup -1}), Ca (0.104 {+-} 0.029gL{sup -1}), Cl (4.41 {+-} 0.25gL{sup -1}), I (76 {+-} 27{mu}gL{sup -1}), K (0.38 {+-} 0.09gL{sup -1}), Mg (0.038 {+-} 0.011gL{sup -1}), Na (4.30 {+-} 0.26gL{sup -1}) and S (1.35 {+-} 0.37gL{sup -1}) were determined for the first time. These data were compared with the concentration values established for Americanum and Anatolicum Excavatum tick species to clarify the ion balance in this biological material (hemolymph). This comparison suggests that Na concentration, majority in these species, has a similar behavior. These data also contribute to the understanding of hemolymph composition complementing its characterization as well as for the understanding of several physiological processes, especially those related to salivary secretion. (author)

  2. Core microbial functional activities in ocean environments revealed by global metagenomic profiling analyses.

    KAUST Repository

    Ferreira, Ari J S

    2014-06-12

    Metagenomics-based functional profiling analysis is an effective means of gaining deeper insight into the composition of marine microbial populations and developing a better understanding of the interplay between the functional genome content of microbial communities and abiotic factors. Here we present a comprehensive analysis of 24 datasets covering surface and depth-related environments at 11 sites around the world\\'s oceans. The complete datasets comprises approximately 12 million sequences, totaling 5,358 Mb. Based on profiling patterns of Clusters of Orthologous Groups (COGs) of proteins, a core set of reference photic and aphotic depth-related COGs, and a collection of COGs that are associated with extreme oxygen limitation were defined. Their inferred functions were utilized as indicators to characterize the distribution of light- and oxygen-related biological activities in marine environments. The results reveal that, while light level in the water column is a major determinant of phenotypic adaptation in marine microorganisms, oxygen concentration in the aphotic zone has a significant impact only in extremely hypoxic waters. Phylogenetic profiling of the reference photic/aphotic gene sets revealed a greater variety of source organisms in the aphotic zone, although the majority of individual photic and aphotic depth-related COGs are assigned to the same taxa across the different sites. This increase in phylogenetic and functional diversity of the core aphotic related COGs most probably reflects selection for the utilization of a broad range of alternate energy sources in the absence of light.

  3. Core microbial functional activities in ocean environments revealed by global metagenomic profiling analyses.

    KAUST Repository

    Ferreira, Ari J S; Siam, Rania; Setubal, Joã o C; Moustafa, Ahmed; Sayed, Ahmed; Chambergo, Felipe S; Dawe, Adam S; Ghazy, Mohamed A; Sharaf, Hazem; Ouf, Amged; Alam, Intikhab; Abdel-Haleem, Alyaa M; Lehvä slaiho, Heikki; Ramadan, Eman; Antunes, André ; Stingl, Ulrich; Archer, John A.C.; Jankovic, Boris R; Sogin, Mitchell; Bajic, Vladimir B.; El-Dorry, Hamza

    2014-01-01

    Metagenomics-based functional profiling analysis is an effective means of gaining deeper insight into the composition of marine microbial populations and developing a better understanding of the interplay between the functional genome content of microbial communities and abiotic factors. Here we present a comprehensive analysis of 24 datasets covering surface and depth-related environments at 11 sites around the world's oceans. The complete datasets comprises approximately 12 million sequences, totaling 5,358 Mb. Based on profiling patterns of Clusters of Orthologous Groups (COGs) of proteins, a core set of reference photic and aphotic depth-related COGs, and a collection of COGs that are associated with extreme oxygen limitation were defined. Their inferred functions were utilized as indicators to characterize the distribution of light- and oxygen-related biological activities in marine environments. The results reveal that, while light level in the water column is a major determinant of phenotypic adaptation in marine microorganisms, oxygen concentration in the aphotic zone has a significant impact only in extremely hypoxic waters. Phylogenetic profiling of the reference photic/aphotic gene sets revealed a greater variety of source organisms in the aphotic zone, although the majority of individual photic and aphotic depth-related COGs are assigned to the same taxa across the different sites. This increase in phylogenetic and functional diversity of the core aphotic related COGs most probably reflects selection for the utilization of a broad range of alternate energy sources in the absence of light.

  4. Learning from clinical placement experience: Analysing nursing students' final reflections in a digital storytelling activity.

    Science.gov (United States)

    Paliadelis, Penny; Wood, Pamela

    2016-09-01

    This paper reports on the learning potential of a reflective activity undertaken by final year nursing students, in which they were asked to recount two meaningful events that occurred during their clinical placements over the duration of their 3-year nursing degree program and reflect on how these events contributed to their learning to become beginning level Registered Nurses (RNs). This descriptive qualitative study gathered narratives from 92 students as individual postings in an online forum created within the University's learning management system. An analysis of the students' reflections are the focus of this paper particularly in relation to the value of reflecting on the identified events. Four themes emerged that clearly highlight the way in which these students interpreted and learned from both positive and negative clinical experiences, their strong desire to fit into their new role and their ability to re-imagine how they might respond to clinical events when they become Registered Nurses. The findings of this study may contribute to developing nursing curricula that better prepares final year students for the realities of practice. Copyright © 2016 Elsevier Ltd. All rights reserved.

  5. Analyses of heart rate variability in young soccer players: the effects of sport activity.

    Science.gov (United States)

    Bricout, Véronique-Aurélie; Dechenaud, Simon; Favre-Juvin, Anne

    2010-04-19

    The use of heart rate variability (HRV) in the management of sport training is a practice which tends to spread, especially in order to prevent the occurrence of states of fatigue. To estimate the HRV parameters obtained using a heart rate recording, according to different loads of sporting activities, and to make the possible link with the appearance of fatigue. Eight young football players, aged 14.6 years+/-2 months, playing at league level in Rhône-Alpes, training for 10 to 20 h per week, were followed over a period of 5 months, allowing to obtain 54 recordings of HRV in three different conditions: (i) after rest (ii) after a day with training and (iii) after a day with a competitive match. Under the effect of a competitive match, the HRV temporal indicators (heart rate, RR interval, and pNN50) were significantly altered compared to the rest day. The analysis of the sympathovagal balance rose significantly as a result of the competitive constraint (0.72+/-0.17 vs. 0.90+/-0.20; pHRV is an objective and non-invasive monitoring of management of the training of young sportsmen. HRV analysis allowed to highlight any neurovegetative adjustments according to the physical loads. Thus, under the effect of an increase of physical and psychological constraints that a football match represents, the LF/HF ratio rises significantly; reflecting increased sympathetic stimulation, which beyond certain limits could be relevant to prevent the emergence of a state of fatigue. 2009 Elsevier B.V. All rights reserved.

  6. Compositions de revêtement réticulables par bombardement électronique. Deuxième partie : Analyse de la littérature des brevets sur la formulation des liants radiodurcissables Forming Crosslinkable Coating by Electron Bombardement. Part Two : Analysis of Patent Literature on Formulating

    Directory of Open Access Journals (Sweden)

    Miléo J. -C.

    2006-11-01

    Full Text Available Le procédé de séchage des peintures et vernis par irradiation électronique est analysé sous son aspect chimique. On passe en revue les différents modes de synthèse permettant d'obtenir les résines radiodurcissables qui font l'objet, sur le plan des brevets, d'une abondante littérature. Ces résines sont classées suivant la nature des insaturations réactives qu'elles contiennent - insaturations de type « ester maléique », - esters et amides (méthacryliques simples; - esters (méthacryliques à enchaînements uréthanes ; - esters (méthacryliques (p-hydroxylés, leurs esters (insaturés et autres dérivés, - siloxanes; - maléimides; - insaturations « allyliques » ; - résines saturées. The process of drying points and varnishes by electron irradiation is analyzed from the chemical standpoint. A review is mode of the different synthesis methods of pro ducing radiation-curable resins that have resulted in abundant patent literature. These resins are classified according ta the nature of the reactive unsaturations they contain - unsaturations of the « maleic ester » type; - simple (methacrylic esters and amides ; - 3-hydroxyl (methacrylic esters, their (unsaturated esters and other derivatives ; - siloxanes ; - maleimides ; - « allylic » unsaturations ; - saturated resins

  7. Serum suPAR in patients with FSGS: trash or treasure?

    Science.gov (United States)

    Maas, Rutger J H; Deegens, Jeroen K J; Wetzels, Jack F M

    2013-07-01

    The urokinase-type plasminogen activator receptor (uPAR) has important functions in cell migration. uPAR can be shed from the cell membrane resulting in soluble uPAR (suPAR). Further cleavage gives rise to shorter fragments with largely unknown functions. Recent studies have demonstrated that both overexpression of uPAR on podocytes and the administration of suPAR cause proteinuria in mice. The common pathogenic mechanism involves the activation of podocyte β3-integrin. Increased activation of β3-integrin is also observed in patients with focal and segmental glomerulosclerosis (FSGS). These observations form the basis for the hypothesis that suPAR may be the circulating factor causing FSGS. A recent study fosters this idea by demonstrating increased suPAR levels in the serum of patients with FSGS and reporting an association with recurrence after transplantation and response to plasmapheresis. However, this study was heavily biased, and subsequent studies have given conflicting results. Although the experimental work is very suggestive, at present there is no proof that any known human suPAR fragment causes FSGS in humans. We therefore suggest that the measurement of suPAR using currently available assays has absolutely no value at the present time in decision-making in routine clinical practice.

  8. Étude de faisabilité de l’estérification d’un diacide par distillation réactive Feasibility Study of Diacid Esterification by Reactive Distillation

    Directory of Open Access Journals (Sweden)

    Steger C.

    2010-09-01

    Full Text Available Cette étude propose une approche systématique de la conception d’une colonne à distiller réactive. Elle a été appliquée avec succès au cas peu étudié des réactions consécutives. L’estérification de l’acide 2 methyl glutarique par le méthanol a été traitée. Les 3 premières étapes de la méthode montrent la faisabilité de la production dans une colonne hybride avec un taux de reflux faible et la nécessité de travailler avec un excès de méthanol. Ces résultats théoriques ont ensuite été consolidés par un pilotage sur une colonne de diamètre 75 mm. Dans tous les régimes pilotés, les résultats obtenus sont significativement supérieurs à un réacteur en lit fixe même avec des excès de réactif plus importants. A systematic methodology for the design of reactive distillation columns has been successfully applied to a mixture with consecutive chemical reactions, i.e. esterification of 2-methyl glutaric acid with methanol. The feasibility of production within a hybride reactive distillation column operated at a low reflux ratio with an excess of methanol has been validated by the first steps of this approach. These results have been experimentally confirmed within a lab-scale reactive distillation column (75 mm inner diameter. Performances are significantly improved for all investigated process conditions in comparison to corresponding fixed bed reactor.

  9. Paracrine Apoptotic Effect of p53 Mediated by Tumor Suppressor Par-4

    Directory of Open Access Journals (Sweden)

    Ravshan Burikhanov

    2014-01-01

    Full Text Available The guardian of the genome, p53, is often mutated in cancer and may contribute to therapeutic resistance. Given that p53 is intact and functional in normal tissues, we harnessed its potential to inhibit the growth of p53-deficient cancer cells. Specific activation of p53 in normal fibroblasts selectively induced apoptosis in p53-deficient cancer cells. This paracrine effect was mediated by p53-dependent secretion of the tumor suppressor Par-4. Accordingly, the activation of p53 in normal mice, but not p53−/− or Par-4−/− mice, caused systemic elevation of Par-4, which induced apoptosis of p53-deficient tumor cells. Mechanistically, p53 induced Par-4 secretion by suppressing the expression of its binding partner, UACA, which sequesters Par-4. Thus, normal cells can be empowered by p53 activation to induce Par-4 secretion for the inhibition of therapy-resistant tumors.

  10. Brahmagupta, Mathematician Par Excellence

    Indian Academy of Sciences (India)

    mathematical and scientific activity for the next millen- nium until the Renaissance in ... (BrSpSi XII.38) This sloka describes the method of obtaining a rational cyclic quadrilateral using two nonsimilar rational right triangles. (This seems to be.

  11. Vote par sondage uniforme incorruptible

    OpenAIRE

    Blanchard , Nicolas

    2016-01-01

    International audience; Introduit en 2012 par David Chaum, le vote par sondage uniforme (random-sample voting) est un protocole de vote basé sur un choix d'une sous-population représentative , permettant de limiter les coûts tout en ayant de nombreux avantages, principalement lorsqu'il est couplé a d'autres techniques comme ThreeBallot. Nous analysons un problème de corruptibilité potentielle où les votants peuvent vendre leur vote au plus offrant et proposons une variation du protocole reméd...

  12. Personal, social, and game-related correlates of active and non-active gaming among dutch gaming adolescents: survey-based multivariable, multilevel logistic regression analyses.

    Science.gov (United States)

    Simons, Monique; de Vet, Emely; Chinapaw, Mai Jm; de Boer, Michiel; Seidell, Jacob C; Brug, Johannes

    2014-04-04

    Playing video games contributes substantially to sedentary behavior in youth. A new generation of video games-active games-seems to be a promising alternative to sedentary games to promote physical activity and reduce sedentary behavior. At this time, little is known about correlates of active and non-active gaming among adolescents. The objective of this study was to examine potential personal, social, and game-related correlates of both active and non-active gaming in adolescents. A survey assessing game behavior and potential personal, social, and game-related correlates was conducted among adolescents (12-16 years, N=353) recruited via schools. Multivariable, multilevel logistic regression analyses, adjusted for demographics (age, sex and educational level of adolescents), were conducted to examine personal, social, and game-related correlates of active gaming ≥1 hour per week (h/wk) and non-active gaming >7 h/wk. Active gaming ≥1 h/wk was significantly associated with a more positive attitude toward active gaming (OR 5.3, CI 2.4-11.8; Pgames (OR 0.30, CI 0.1-0.6; P=.002), a higher score on habit strength regarding gaming (OR 1.9, CI 1.2-3.2; P=.008) and having brothers/sisters (OR 6.7, CI 2.6-17.1; Pgame engagement (OR 0.95, CI 0.91-0.997; P=.04). Non-active gaming >7 h/wk was significantly associated with a more positive attitude toward non-active gaming (OR 2.6, CI 1.1-6.3; P=.035), a stronger habit regarding gaming (OR 3.0, CI 1.7-5.3; P7 h/wk. Active gaming is most strongly (negatively) associated with attitude with respect to non-active games, followed by observed active game behavior of brothers and sisters and attitude with respect to active gaming (positive associations). On the other hand, non-active gaming is most strongly associated with observed non-active game behavior of friends, habit strength regarding gaming and attitude toward non-active gaming (positive associations). Habit strength was a correlate of both active and non-active gaming

  13. Personal, Social, and Game-Related Correlates of Active and Non-Active Gaming Among Dutch Gaming Adolescents: Survey-Based Multivariable, Multilevel Logistic Regression Analyses

    Science.gov (United States)

    de Vet, Emely; Chinapaw, Mai JM; de Boer, Michiel; Seidell, Jacob C; Brug, Johannes

    2014-01-01

    Background Playing video games contributes substantially to sedentary behavior in youth. A new generation of video games—active games—seems to be a promising alternative to sedentary games to promote physical activity and reduce sedentary behavior. At this time, little is known about correlates of active and non-active gaming among adolescents. Objective The objective of this study was to examine potential personal, social, and game-related correlates of both active and non-active gaming in adolescents. Methods A survey assessing game behavior and potential personal, social, and game-related correlates was conducted among adolescents (12-16 years, N=353) recruited via schools. Multivariable, multilevel logistic regression analyses, adjusted for demographics (age, sex and educational level of adolescents), were conducted to examine personal, social, and game-related correlates of active gaming ≥1 hour per week (h/wk) and non-active gaming >7 h/wk. Results Active gaming ≥1 h/wk was significantly associated with a more positive attitude toward active gaming (OR 5.3, CI 2.4-11.8; Pgames (OR 0.30, CI 0.1-0.6; P=.002), a higher score on habit strength regarding gaming (OR 1.9, CI 1.2-3.2; P=.008) and having brothers/sisters (OR 6.7, CI 2.6-17.1; Pgame engagement (OR 0.95, CI 0.91-0.997; P=.04). Non-active gaming >7 h/wk was significantly associated with a more positive attitude toward non-active gaming (OR 2.6, CI 1.1-6.3; P=.035), a stronger habit regarding gaming (OR 3.0, CI 1.7-5.3; P7 h/wk. Active gaming is most strongly (negatively) associated with attitude with respect to non-active games, followed by observed active game behavior of brothers and sisters and attitude with respect to active gaming (positive associations). On the other hand, non-active gaming is most strongly associated with observed non-active game behavior of friends, habit strength regarding gaming and attitude toward non-active gaming (positive associations). Habit strength was a

  14. Radioisotope x-ray fluorescence and neutron activation analyses of the trace element concentrations of the rainbow trout

    International Nuclear Information System (INIS)

    Akyuz, T.; Bassari, A.; Bolcal, C.; Sener, E.; Yildiz, M.; Kucer, R.; Kaplan, Z.; Dogan, G.; Akyuz, S.

    1999-01-01

    The muscles and livers of the ten rainbow trouts (Oncorhynchus mykiss; N, 1752) obtained from Sapanca, Aquaculture Facility of Aquatic Products Faculty, The University of Istanbul (Turkey), have been analysed quantitatively for some minor elements using the radioisotope energy dispersive X-ray fluorescence (EDXRF) and neutron activation analysis (NAA) methods. It was found that samples contain Na, K, Ca, Sc, Cs, Fe, Co, Cu, Zn, Se, Br, Rb, Sr, Au, La and Ce in different amounts. Comparison of the results with those of reference river fish samples indicated that agricultural rainbow trout samples from Sapanca region have higher Fe level. (author)

  15. Radioisotope X-ray fluorescence and neutron activation analyses of the trace element concentrations of the rainbow trout

    Science.gov (United States)

    Akyuz, T.; Bassari, A.; Bolcal, C.; Sener, E.; Yildiz, M.; Kucer, R.; Kaplan, Z.; Dogan, G.; Akyuz, S.

    1999-01-01

    The muscles and livers of the ten rainbow trouts ( Oncorhynchus mykiss; N, 1752) obtained from Sapanca, Aquaculture Facility of Aquatic Products Faculty, The University of Istanbul (Turkey), have been analysed quantitatively for some minor elements using the radioisotope energy dispersive X-ray fluorescence (EDXRF) and neutron activation analysis (NAA) methods. It was found that samples contain Na, K, Ca, Sc, Cs, Fe, Co, Cu, Zn, Se, Br, Rb, Sr, Au, La and Ce in different amounts. Comparison of the results with those of reference river fish samples indicated that agricultural rainbow trout samples from Sapanca region have higher Fe level.

  16. PAR-2 expression in the gingival crevicular fluid reflects chronic periodontitis severity

    Directory of Open Access Journals (Sweden)

    Henrique FUKUSHIMA

    Full Text Available Abstract Recent studies investigating protease-activated receptor type 2 (PAR-2 suggest an association between the receptor and periodontal inflammation. It is known that gingipain, a bacterial protease secreted by the important periodontopathogen Porphyromonas gingivalis can activate PAR-2. Previous studies by our group found that PAR-2 is overexpressed in the gingival crevicular fluid (GCF of patients with moderate chronic periodontitis (MP. The present study aimed at evaluating whether PAR-2 expression is associated with chronic periodontitis severity. GCF samples and clinical parameters, including plaque and bleeding on probing indices, probing pocket depth and clinical attachment level, were collected from the control group (n = 19 at baseline, and from MP patients (n = 19 and severe chronic periodontitis (SP (n = 19 patients before and 6 weeks after periodontal non-surgical treatment. PAR-2 and gingipain messenger RNA (mRNA in the GCF of 4 periodontal sites per patient were evaluated by Reverse Transcription Polymerase Chain Reaction (RT-qPCR. PAR-2 and gingipain expressions were greater in periodontitis patients than in control group patients. In addition, the SP group presented increased PAR-2 and gingipain mRNA levels, compared with the MP group. Furthermore, periodontal treatment significantly reduced (p <0.05 PAR-2 expression in patients with periodontitis. In conclusion, PAR-2 is associated with chronic periodontitis severity and with gingipain levels in the periodontal pocket, thus suggesting that PAR-2 expression in the GCF reflects the severity of destruction during periodontal infection.

  17. PAR-1 contributes to the innate immune response during viral infection

    Science.gov (United States)

    Antoniak, Silvio; Owens, A. Phillip; Baunacke, Martin; Williams, Julie C.; Lee, Rebecca D.; Weithäuser, Alice; Sheridan, Patricia A.; Malz, Ronny; Luyendyk, James P.; Esserman, Denise A.; Trejo, JoAnn; Kirchhofer, Daniel; Blaxall, Burns C.; Pawlinski, Rafal; Beck, Melinda A.; Rauch, Ursula; Mackman, Nigel

    2013-01-01

    Coagulation is a host defense system that limits the spread of pathogens. Coagulation proteases, such as thrombin, also activate cells by cleaving PARs. In this study, we analyzed the role of PAR-1 in coxsackievirus B3–induced (CVB3-induced) myocarditis and influenza A infection. CVB3-infected Par1–/– mice expressed reduced levels of IFN-β and CXCL10 during the early phase of infection compared with Par1+/+ mice that resulted in higher viral loads and cardiac injury at day 8 after infection. Inhibition of either tissue factor or thrombin in WT mice also significantly increased CVB3 levels in the heart and cardiac injury compared with controls. BM transplantation experiments demonstrated that PAR-1 in nonhematopoietic cells protected mice from CVB3 infection. Transgenic mice overexpressing PAR-1 in cardiomyocytes had reduced CVB3-induced myocarditis. We found that cooperative signaling between PAR-1 and TLR3 in mouse cardiac fibroblasts enhanced activation of p38 and induction of IFN-β and CXCL10 expression. Par1–/– mice also had decreased CXCL10 expression and increased viral levels in the lung after influenza A infection compared with Par1+/+ mice. Our results indicate that the tissue factor/thrombin/PAR-1 pathway enhances IFN-β expression and contributes to the innate immune response during single-stranded RNA viral infection. PMID:23391721

  18. Psychosocial versus physiological stress – meta-analyses on deactivations and activations of the neural correlates of stress reactions

    Science.gov (United States)

    Kogler, Lydia; Mueller, Veronika I.; Chang, Amy; Eickhoff, Simon B.; Fox, Peter T.; Gur, Ruben C.; Derntl, Birgit

    2015-01-01

    Stress is present in everyday life in various forms and situations. Two stressors frequently investigated are physiological and psychosocial stress. Besides similar subjective and hormonal responses, it has been suggested that they also share common neural substrates. The current study used activation-likelihood-estimation meta-analysis to test this assumption by integrating results of previous neuroimaging studies on stress processing. Reported results are cluster-level FWE corrected. The inferior frontal gyrus (IFG) and the anterior insula (AI) were the only regions that demonstrated overlapping activation for both stressors. Analysis of physiological stress showed consistent activation of cognitive and affective components of pain processing such as the insula, striatum, or the middle cingulate cortex. Contrarily, analysis across psychosocial stress revealed consistent activation of the right superior temporal gyrus and deactivation of the striatum. Notably, parts of the striatum appeared to be functionally specified: the dorsal striatum was activated in physiological stress, whereas the ventral striatum was deactivated in psychosocial stress. Additional functional connectivity and decoding analyses further characterized this functional heterogeneity and revealed higher associations of the dorsal striatum with motor regions and of the ventral striatum with reward processing. Based on our meta-analytic approach, activation of the IFG and the AI seems to indicate a global neural stress reaction. While physiological stress activates a motoric fight-or-flight reaction, during psychosocial stress attention is shifted towards emotion regulation and goal-directed behavior, and reward processing is reduced. Our results show the significance of differentiating physiological and psychosocial stress in neural engagement. Furthermore, the assessment of deactivations in addition to activations in stress research is highly recommended. PMID:26123376

  19. Determinations of elements in pepperbush standard reference material by neutron activation and X-ray fluorescence analyses

    International Nuclear Information System (INIS)

    Mizumoto, Yoshihiko; Okada, Takayuki; Tatsumi, Toshiya; Kusakabe, Toshio; Katsurayama, Kousuke; Iwata, Shiro.

    1988-01-01

    Elemental contents in Pepperbush standard reference material have been determined by neutron activation and X-ray fluorescence analyses. The standard samples of orchard leaves, tomato leaves, pine needles and Kale are used for the experiment. In the neutron activation analysis, gamma-ray spectra of nuclei produced by (n,γ) reaction on Pepperbush and standard samples are measured with Ge detectors. In the X-ray fluorescence analysis, the samples are excited with X-rays from X-ray tube with rhodium anode, and the characteristic X-rays from samples are measured with a proportional counter or NaI(Tl) detector. From the gamma- and X-ray intensities, the elemental contents in Pepperbush are determined. As a result, the contents of seventeen elements, such as sodium, calcium, iron, etc., in Pepperbush are determined. (author)

  20. [Ambroise Paré, his death and his historians].

    Science.gov (United States)

    Dumaître, P

    2001-01-01

    Ambroise Paré died December the 20th., 1590. What happened after his death and what are we knowing about his life? Never forgotten, though a lack of care, we were waiting till the XIXth. century to get recollection upon a subject sustained only by accounts of his travels. After Percy, a surgeon who tried to write Paré's biography through the "Biographie universelle ancienne et moderne, de Michaud (1822)" and some second rank authors, Malgaigne is the first who inside a printing of the "Oeuvres complétes de Paré (1840-41)" has a fine look upon the question, though his work is incomplete and allows many mistakes. Doctors Chéreau and Jal, an historian, analysing civil status records, gave new information enlarged by doctors Le Paulmier, Turner and the U.S. native Janet Doe.

  1. Cross-sectional associations of active transport, employment status and objectively measured physical activity: analyses from the National Health and Nutrition Examination Survey.

    Science.gov (United States)

    Yang, Lin; Hu, Liang; Hipp, J Aaron; Imm, Kellie R; Schutte, Rudolph; Stubbs, Brendon; Colditz, Graham A; Smith, Lee

    2018-05-05

    To investigate associations between active transport, employment status and objectively measured moderate-to-vigorous physical activity (MVPA) in a representative sample of US adults. Cross-sectional analyses of data from the National Health and Nutrition Examination Survey. A total of 5180 adults (50.2 years old, 49.0% men) were classified by levels of active transportation and employment status. Outcome measure was weekly time spent in MVPA as recorded by the Actigraph accelerometer. Associations between active transport, employment status and objectively measured MVPA were examined using multivariable linear regression models adjusted for age, body mass index, race and ethnicity, education level, marital status, smoking status, working hour duration (among the employed only) and self-reported leisure time physical activity. Patterns of active transport were similar between the employed (n=2897) and unemployed (n=2283), such that 76.0% employed and 77.5% unemployed engaged in no active transport. For employed adults, those engaging in high levels of active transport (≥90 min/week) had higher amount of MVPA than those who did not engage in active transport. This translated to 40.8 (95% CI 15.7 to 65.9) additional minutes MVPA per week in men and 57.9 (95% CI 32.1 to 83.7) additional minutes MVPA per week in women. Among the unemployed adults, higher levels of active transport were associated with more MVPA among men (44.8 min/week MVPA, 95% CI 9.2 to 80.5) only. Findings from the present study support interventions to promote active transport to increase population level physical activity. Additional strategies are likely required to promote physical activity among unemployed women. © Article author(s) (or their employer(s) unless otherwise stated in the text of the article) 2018. All rights reserved. No commercial use is permitted unless otherwise expressly granted.

  2. The Determination of Components of Radioactive Decay Mixtures by Computer Analysis of Count-Rate Data; Determination des Composants de Melanges Radioactifs en Decroissance par Analyse a l'Ordinateur des Resultats du Comptage; Opredelenie komponentov smesej radioaktivnogo raspada posredstvom analiza dannykh skorostej scheta s pomoshch'yu schetno-reshayushchego ustrojstva; Determinacion de los Componentes de Mezclas Radiactivas por Analisis de los Datos de Recuento Mediante Calculadoras

    Energy Technology Data Exchange (ETDEWEB)

    Airman, W. D.F.; Tyler, S. A.; Dipertand, M. H.; Sedlet, J. [Argonne National Laboratory, Argonne, IL (United States)

    1965-10-15

    The components of a mixture of n radioactive isotopes can be determined from the change in activity with time provided that the activity of at least n-1 components changes significantly during the period of observation, either by direct decay or by the growth of decay products. ft is possible to predict a set of possible components for each mixture encountered, based on considerations such as the origin and history of the mixture and the separation chemistry and counting technique(s) used. If such considerations are properly applied, the set of possible components will include all of the actual components in the mixture. The appropriate growth and/or decay equations can then be formulated and solved simultaneously to obtain each component, or the mixture can be resolved graphically by extrapolations of the linear portions of the total decay-growth curve. However, when the number of components is large and/or when complex decay schemes are involved, these two techniques either cannot be applied or the errors associated with the estimates cannot be assessed. Selection of decay components by a least-squares procedure provides better estimates than solution by simultaneous equations alone. Consequently, a least-squares Fortran computer programme (designated CORD) has been developed which solves the general problem: given the times and counts per unit time from a sample, the possible radioisotopic parents and decay schemes and all associated decay constants and detection efficiencies compute the amount of each parent actually present at a predetermined zero time. In addition, the programme yields the amounts of the parents and daughters present at all data times. Initially used with bioassay and environmental samples, the programme has been specifically designed for analysing count-rate data obtained by non-spectroscopic alpha- or beta-counting. However, it should be adaptable to total gamma and spectroscopic data, provided the energy ranges over which these

  3. Genomewide analyses define different modes of transcriptional regulation by peroxisome proliferator-activated receptor-β/δ (PPARβ/δ.

    Directory of Open Access Journals (Sweden)

    Till Adhikary

    Full Text Available Peroxisome proliferator-activated receptors (PPARs are nuclear receptors with essential functions in lipid, glucose and energy homeostasis, cell differentiation, inflammation and metabolic disorders, and represent important drug targets. PPARs heterodimerize with retinoid X receptors (RXRs and can form transcriptional activator or repressor complexes at specific DNA elements (PPREs. It is believed that the decision between repression and activation is generally governed by a ligand-mediated switch. We have performed genomewide analyses of agonist-treated and PPARβ/δ-depleted human myofibroblasts to test this hypothesis and to identify global principles of PPARβ/δ-mediated gene regulation. Chromatin immunoprecipitation sequencing (ChIP-Seq of PPARβ/δ, H3K4me3 and RNA polymerase II enrichment sites combined with transcriptional profiling enabled the definition of 112 bona fide PPARβ/δ target genes showing either of three distinct types of transcriptional response: (I ligand-independent repression by PPARβ/δ; (II ligand-induced activation and/or derepression by PPARβ/δ; and (III ligand-independent activation by PPARβ/δ. These data identify PPRE-mediated repression as a major mechanism of transcriptional regulation by PPARβ/δ, but, unexpectedly, also show that only a subset of repressed genes are activated by a ligand-mediated switch. Our results also suggest that the type of transcriptional response by a given target gene is connected to the structure of its associated PPRE(s and the biological function of its encoded protein. These observations have important implications for understanding the regulatory PPAR network and PPARβ/δ ligand-based drugs.

  4. Dosimetric optimization approach for activities of metal plutonium elaboration by calciothermy; Demarche d'optimisation dosimetrique des activites d'elaboration du plutonium metal par calciothermie

    Energy Technology Data Exchange (ETDEWEB)

    Crovisier, Ph.; Brunetti, L.; Jourde, S.; Piot, J.; Valier Brasier, P. [CEA/DAMNA/DSTA - Service de Protection contre les Rayonnements- Centre de Valduc - 21120 Is sur Tille (France)

    2008-07-01

    The authors present the activities performed within a new unit of a plutonium pyro-chemistry laboratory, report a dosimetric assessment of workstation in this unit, compare the data with those obtained with the previous installation, and report a study of the neutronic efficiency of biological protection shields (measurements of neutron spectra)

  5. Structures of actin-like ParM filaments show architecture of plasmid-segregating spindles.

    Science.gov (United States)

    Bharat, Tanmay A M; Murshudov, Garib N; Sachse, Carsten; Löwe, Jan

    2015-07-02

    Active segregation of Escherichia coli low-copy-number plasmid R1 involves formation of a bipolar spindle made of left-handed double-helical actin-like ParM filaments. ParR links the filaments with centromeric parC plasmid DNA, while facilitating the addition of subunits to ParM filaments. Growing ParMRC spindles push sister plasmids to the cell poles. Here, using modern electron cryomicroscopy methods, we investigate the structures and arrangements of ParM filaments in vitro and in cells, revealing at near-atomic resolution how subunits and filaments come together to produce the simplest known mitotic machinery. To understand the mechanism of dynamic instability, we determine structures of ParM filaments in different nucleotide states. The structure of filaments bound to the ATP analogue AMPPNP is determined at 4.3 Å resolution and refined. The ParM filament structure shows strong longitudinal interfaces and weaker lateral interactions. Also using electron cryomicroscopy, we reconstruct ParM doublets forming antiparallel spindles. Finally, with whole-cell electron cryotomography, we show that doublets are abundant in bacterial cells containing low-copy-number plasmids with the ParMRC locus, leading to an asynchronous model of R1 plasmid segregation.

  6. Par Pond Fish, Water, and Sediment Chemistry

    Energy Technology Data Exchange (ETDEWEB)

    Paller, M.H. [Westinghouse Savannah River Company, AIKEN, SC (United States); Wike, L.D.

    1996-06-01

    The objectives of this report are to describe the Par Pond fish community and the impact of the drawdown and refill on the community, describe contaminant levels in Par Pond fish, sediments, and water and indicate how contaminant concentrations and distributions were affected by the drawdown and refill, and predict possible effects of future water level fluctuations in Par Pond.

  7. Par Pond Fish, Water, and Sediment Chemistry

    International Nuclear Information System (INIS)

    Paller, M.H.; Wike, L.D.

    1996-06-01

    The objectives of this report are to describe the Par Pond fish community and the impact of the drawdown and refill on the community, describe contaminant levels in Par Pond fish, sediments, and water and indicate how contaminant concentrations and distributions were affected by the drawdown and refill, and predict possible effects of future water level fluctuations in Par Pond

  8. par genes in Mycobacterium bovis and Mycobacterium smegmatis are arranged in an operon transcribed from "SigGC" promoters

    Directory of Open Access Journals (Sweden)

    Casart Yveth

    2008-03-01

    Full Text Available Abstract Background The ParA/Soj and ParB/Spo0J proteins, and the cis-acting parS site, participate actively in chromosome segregation and cell cycle progression. Genes homologous to parA and parB, and two putative parS copies, have been identified in the Mycobacterium bovis BCG and Mycobacterium smegmatis chromosomes. As in Mycobacterium tuberculosis, the parA and parB genes in these two non-pathogenic mycobacteria are located near the chromosomal origin of replication. The present work focused on the determination of the transcriptional organisation of the ~6 Kb orf60K-parB region of M. bovis BCG and M. smegmatis by primer extension, transcriptional fusions to the green fluorescence protein (GFP and quantitative RT-PCR. Results The parAB genes were arranged in an operon. However, we also found promoters upstream of each one of these genes. Seven putative promoter sequences were identified in the orf60K-parB region of M. bovis BCG, whilst four were identified in the homologous region of M. smegmatis, one upstream of each open reading frame (ORF. Real-time PCR assays showed that in M. smegmatis, mRNA-parA and mRNA-parB levels decreased between the exponential and stationary phases. In M. bovis BCG, mRNA-parA levels also decreased between the exponential and stationary phases. However, parB expression was higher than parA expression and remained almost unchanged along the growth curve. Conclusion The majority of the proposed promoter regions had features characteristic of Mycobacterium promoters previously denoted as Group D. The -10 hexamer of a strong E. coli σ70-like promoter, located upstream of gidB of M. bovis BCG, overlapped with a putative parS sequence, suggesting that the transcription from this promoter might be regulated by the binding of ParB to parS.

  9. Determination of polyphenolic content, HPLC analyses and DNA cleavage activity of Malaysian Averrhoa carambola L. fruit extracts

    Directory of Open Access Journals (Sweden)

    Zakia Khanam

    2015-10-01

    Full Text Available In developing countries, the increasing gap between population growth and food supply has created renewed interest in finding reliable and cheap natural resources of nutraceutical value and health promoting properties. Therefore, the present study deals with the phytochemical analyses and DNA cleavage activity of Averrhoa carambola L. fruit (starfruit extracts. The phytochemical studies involve colour tests and quantification of phenolics and flavonoids of the prepared ethanolic and aqueous extracts. Identification of phenolic acids and flavonoids present in the extracts were conducted by high performance liquid chromatography (HPLC equipped with diode array detector (DAD. DNA cleavage activity of the extracts was evaluated through gel electrophoresis against plasmid Escherichia coli DNA at different concentrations (0.125–0.60 μg/μl. The results of the study exhibited that the starfruit is a rich source of polyphenols and all the extracts exhibited a dose dependent DNA cleavage activity, whereas ethanolic extract induced more cleavage as compared to the aqueous extract. In conclusion, the present study provides preliminary evidence with regard to nutraceutical value of the fruit. So, further extensive study is a prerequisite to exploit DNA cleaving properties of the fruit extracts for therapeutic application.

  10. Paternal age related schizophrenia (PARS): Latent subgroups detected by k-means clustering analysis.

    Science.gov (United States)

    Lee, Hyejoo; Malaspina, Dolores; Ahn, Hongshik; Perrin, Mary; Opler, Mark G; Kleinhaus, Karine; Harlap, Susan; Goetz, Raymond; Antonius, Daniel

    2011-05-01

    Paternal age related schizophrenia (PARS) has been proposed as a subgroup of schizophrenia with distinct etiology, pathophysiology and symptoms. This study uses a k-means clustering analysis approach to generate hypotheses about differences between PARS and other cases of schizophrenia. We studied PARS (operationally defined as not having any family history of schizophrenia among first and second-degree relatives and fathers' age at birth ≥ 35 years) in a series of schizophrenia cases recruited from a research unit. Data were available on demographic variables, symptoms (Positive and Negative Syndrome Scale; PANSS), cognitive tests (Wechsler Adult Intelligence Scale-Revised; WAIS-R) and olfaction (University of Pennsylvania Smell Identification Test; UPSIT). We conducted a series of k-means clustering analyses to identify clusters of cases containing high concentrations of PARS. Two analyses generated clusters with high concentrations of PARS cases. The first analysis (N=136; PARS=34) revealed a cluster containing 83% PARS cases, in which the patients showed a significant discrepancy between verbal and performance intelligence. The mean paternal and maternal ages were 41 and 33, respectively. The second analysis (N=123; PARS=30) revealed a cluster containing 71% PARS cases, of which 93% were females; the mean age of onset of psychosis, at 17.2, was significantly early. These results strengthen the evidence that PARS cases differ from other patients with schizophrenia. Hypothesis-generating findings suggest that features of PARS may include a discrepancy between verbal and performance intelligence, and in females, an early age of onset. These findings provide a rationale for separating these phenotypes from others in future clinical, genetic and pathophysiologic studies of schizophrenia and in considering responses to treatment. Copyright © 2011 Elsevier B.V. All rights reserved.

  11. Les difficultés de recrutement : quelques éléments d’analyse sur la perception du phénomène par les DRH des entreprises de la région de Québec

    Directory of Open Access Journals (Sweden)

    Mircea Vultur

    2009-10-01

    Full Text Available À partir de l’analyse des résultats d’une enquête qualitative auprès de 30 entreprises situées dans la région de Québec, cet article présente les perceptions et les représentations des directeurs des ressources humaines (DRH relatives aux difficultés de recrutement de la main-d’œuvre. Dans un premier temps, j’identifierai les difficultés de recrutement en fonction de leurs caractéristiques, illustrant la réalité observée par les DRH. Dans un deuxième temps, je mettrai en évidence les facteurs explicatifs des difficultés de recrutement tels qu’ils ressortent du discours des DRH, en les regroupant en trois catégories : facteurs de nature exogène, facteurs liés à l’offre de travail et facteurs liés à la demande de travail. En conclusion, j’ouvrirai une brève discussion sur l’importance relative des facteurs explicatifs des difficultés de recrutement et sur les pistes de solution possibles pour faire face à ce phénomène.Based upon the analysis of the results of a qualitative investigation conducted among 30 companies located in the area of Quebec city, this article presents Human Resources Directors (HRD’ perceptions and representations relating to recruitment difficulties. Initially, I will identify recruitment difficulties according to their characteristics, illustrating the reality observed by the HRD. Secondly, I will highlight the explanatory factors underlying recruitment difficulties such as they emerge from HRD’s discourse, by grouping them in three categories: exogenous factors, factors related to work supply and factors dependent on work demand. In conclusion, I will initiate a short discussion on the relative importance of the explanatory factors underlying recruitment difficulties and on the possible solutions to face this phenomenon.

  12. Reflection About the Necessity of Amending Art. 8 par. 2a of the Social Security System Act on the Example of the Medical Activity

    Directory of Open Access Journals (Sweden)

    Damian Wąsik

    2014-12-01

    Full Text Available The publication discusses the problem of a controversial interpretation of art. 8 paragraph. 2a of the Social Security Act, requiring hospital treatment as actual beneficiaries “work” of doctors and nurses (midwives on duty under civil law contracts - signed with a third party. The author analyzes the accuracy of the interpretation from the perspective of medical activity assumptions, which specificity seems to be so far marginalized in the process of applying the law. The result of the author's considerations are demands to work on the amendment of the law on social insurance system taking into account the specific conditions of the exercise of the medical profession.

  13. Molecular and functional analyses of a maize autoactive NB-LRR protein identify precise structural requirements for activity.

    Directory of Open Access Journals (Sweden)

    Guan-Feng Wang

    2015-02-01

    Full Text Available Plant disease resistance is often mediated by nucleotide binding-leucine rich repeat (NLR proteins which remain auto-inhibited until recognition of specific pathogen-derived molecules causes their activation, triggering a rapid, localized cell death called a hypersensitive response (HR. Three domains are recognized in one of the major classes of NLR proteins: a coiled-coil (CC, a nucleotide binding (NB-ARC and a leucine rich repeat (LRR domains. The maize NLR gene Rp1-D21 derives from an intergenic recombination event between two NLR genes, Rp1-D and Rp1-dp2 and confers an autoactive HR. We report systematic structural and functional analyses of Rp1 proteins in maize and N. benthamiana to characterize the molecular mechanism of NLR activation/auto-inhibition. We derive a model comprising the following three main features: Rp1 proteins appear to self-associate to become competent for activity. The CC domain is signaling-competent and is sufficient to induce HR. This can be suppressed by the NB-ARC domain through direct interaction. In autoactive proteins, the interaction of the LRR domain with the NB-ARC domain causes de-repression and thus disrupts the inhibition of HR. Further, we identify specific amino acids and combinations thereof that are important for the auto-inhibition/activity of Rp1 proteins. We also provide evidence for the function of MHD2, a previously uncharacterized, though widely conserved NLR motif. This work reports several novel insights into the precise structural requirement for NLR function and informs efforts towards utilizing these proteins for engineering disease resistance.

  14. Molecular and functional analyses of a maize autoactive NB-LRR protein identify precise structural requirements for activity.

    Science.gov (United States)

    Wang, Guan-Feng; Ji, Jiabing; El-Kasmi, Farid; Dangl, Jeffery L; Johal, Guri; Balint-Kurti, Peter J

    2015-02-01

    Plant disease resistance is often mediated by nucleotide binding-leucine rich repeat (NLR) proteins which remain auto-inhibited until recognition of specific pathogen-derived molecules causes their activation, triggering a rapid, localized cell death called a hypersensitive response (HR). Three domains are recognized in one of the major classes of NLR proteins: a coiled-coil (CC), a nucleotide binding (NB-ARC) and a leucine rich repeat (LRR) domains. The maize NLR gene Rp1-D21 derives from an intergenic recombination event between two NLR genes, Rp1-D and Rp1-dp2 and confers an autoactive HR. We report systematic structural and functional analyses of Rp1 proteins in maize and N. benthamiana to characterize the molecular mechanism of NLR activation/auto-inhibition. We derive a model comprising the following three main features: Rp1 proteins appear to self-associate to become competent for activity. The CC domain is signaling-competent and is sufficient to induce HR. This can be suppressed by the NB-ARC domain through direct interaction. In autoactive proteins, the interaction of the LRR domain with the NB-ARC domain causes de-repression and thus disrupts the inhibition of HR. Further, we identify specific amino acids and combinations thereof that are important for the auto-inhibition/activity of Rp1 proteins. We also provide evidence for the function of MHD2, a previously uncharacterized, though widely conserved NLR motif. This work reports several novel insights into the precise structural requirement for NLR function and informs efforts towards utilizing these proteins for engineering disease resistance.

  15. PAR-2 expression in the gingival crevicular fluid reflects chronic periodontitis severity.

    Science.gov (United States)

    Fukushima, Henrique; Alves, Vanessa Tubero Euzebio; Carvalho, Verônica Franco de; Ambrósio, Lucas Macedo Batitucci; Eichler, Rosangela Aparecida Dos Santos; Carvalho, Maria Helena Catelli de; Saraiva, Luciana; Holzhausen, Marinella

    2017-01-26

    Recent studies investigating protease-activated receptor type 2 (PAR-2) suggest an association between the receptor and periodontal inflammation. It is known that gingipain, a bacterial protease secreted by the important periodontopathogen Porphyromonas gingivalis can activate PAR-2. Previous studies by our group found that PAR-2 is overexpressed in the gingival crevicular fluid (GCF) of patients with moderate chronic periodontitis (MP). The present study aimed at evaluating whether PAR-2 expression is associated with chronic periodontitis severity. GCF samples and clinical parameters, including plaque and bleeding on probing indices, probing pocket depth and clinical attachment level, were collected from the control group (n = 19) at baseline, and from MP patients (n = 19) and severe chronic periodontitis (SP) (n = 19) patients before and 6 weeks after periodontal non-surgical treatment. PAR-2 and gingipain messenger RNA (mRNA) in the GCF of 4 periodontal sites per patient were evaluated by Reverse Transcription Polymerase Chain Reaction (RT-qPCR). PAR-2 and gingipain expressions were greater in periodontitis patients than in control group patients. In addition, the SP group presented increased PAR-2 and gingipain mRNA levels, compared with the MP group. Furthermore, periodontal treatment significantly reduced (p periodontitis. In conclusion, PAR-2 is associated with chronic periodontitis severity and with gingipain levels in the periodontal pocket, thus suggesting that PAR-2 expression in the GCF reflects the severity of destruction during periodontal infection.

  16. Mouse and human genetic analyses associate kalirin with ventral striatal activation during impulsivity and with alcohol misuse

    Directory of Open Access Journals (Sweden)

    Yolanda ePeña-Oliver

    2016-04-01

    Full Text Available Impulsivity is associated with a spectrum of psychiatric disorders including drug addiction. To investigate genetic associations with impulsivity and initiation of drug taking, we took a two-step approach. First, we identified genes whose expression level in prefrontal cortex, striatum and accumbens were associated with impulsive behaviour in the 5-choice serial reaction time task across 10 BXD recombinant inbred (BXD RI mouse strains and their progenitor C57BL/6J and DBA2/J strains. Behavioural data were correlated with regional gene expression using GeneNetwork (www.genenetwork.org, to identify 44 genes whose probability of association with impulsivity exceeded a false discovery rate of <0.05. We then interrogated the IMAGEN database of 1423 adolescents for potential associations of SNPs in human homologues of those genes identified in the mouse study, with brain activation during impulsive performance in the Monetary Incentive Delay task, and with novelty seeking scores from the Temperament and Character Inventory, as well as alcohol-experience. There was a significant overall association between the human homologues of impulsivity-related genes and percentage of premature responses in the MID task and with fMRI BOLD-response in ventral striatum (VS during reward anticipation. In contrast, no significant association was found between the polygenic scores and anterior cingulate cortex activation. Univariate association analyses revealed that the G allele (major of the intronic SNP rs6438839 in the KALRN gene was significantly associated with increased VS activation. Additionally, the A-allele (minor of KALRN intronic SNP rs4634050, belonging to the same haplotype block, was associated with increased frequency of binge drinking.

  17. Locomotor activity measures in the diagnosis of Attention Deficit Hyperactivity Disorder: Meta-analyses and new findings

    Science.gov (United States)

    Murillo, Lourdes García; Cortese, Samuele; Anderson, David; Martino, Adriana Di; Castellanos, Francisco Xavier

    2015-01-01

    Introduction Our aim was to assess differences in movement measures in Attention-Deficit/Hyperactivity Disorder (ADHD) vs. typically developing (TD) controls. Methods We performed meta-analyses of published studies on motion measures contrasting ADHD with controls. We also conducted a case-control study with children/adolescents (n=61 TD, n=62 ADHD) and adults (n=30 TD, n=19 ADHD) using the McLean Motion Activity Test, semi-structured diagnostic interviews and the Behavior Rating Inventory of Executive Function and Conners (Parent, Teacher; Self) Rating Scales. Results Meta-analyses revealed medium-to-large effect sizes for actigraph (standardized mean difference [SMD]: 0.64, 95% Confidence interval (CI): 0.43, 0.85) and motion tracking systems (SDM: 0.92, 95% CI: 0.65, 1.20) measures in differentiating individuals with ADHD from controls. Effects sizes were similar in studies of children/adolescents ([SMD]:0.75, 95% CI: 0.50, 1.01) and of adults ([SMD]: 0.73, 95% CI: 0.46, 1.00). In our sample, ADHD groups differed significantly in number of Head Movements (p=0.02 in children; p=0.002 in adults), Displacement (p=0.009/pADHD (d=0.45, 95% CI: 0.08, 0.82). In the concurrent go/no-go task, reaction time variability was significantly greater in ADHD (pADHD even in adults. Our results suggest that objective locomotion measures may be particularly useful in evaluating adults with possible ADHD. PMID:25770940

  18. Alterações morfológicas de tecido laminar do casco e parâmetros clínicos e laboratoriais de equinos com síndrome cólica letal Morphologic alterations of the hoof lamellar tissue, and clinic and laboratorial analyses of horses with lethal colic syndrome

    Directory of Open Access Journals (Sweden)

    Luciane M. Laskoski

    2009-09-01

    Full Text Available As afecções gastrintestinais dos cavalos são agravadas por complicações como a laminite, cuja etiopatogenia está relacionada à degradação da membrana basal do tecido laminar por metaloproteinases (MMPs. A ativação das MMPs pode ocorrer devido à liberação local de citocinas inflamatórias ou enzimas provenientes de leucócitos infiltrados no tecido laminar. O objetivo deste trabalho foi avaliar as alterações morfológicas do tecido laminar de equinos com síndrome cólica letal e sua provável associação com parâmetros clínicos e laboratoriais. Observou-se intensa destruição da arquitetura laminar, principalmente nos animais com alterações físicas e laboratoriais mais acentuadas, como tempo de preenchimento capilar prolongado (TPC, membranas mucosas congestas, taquicardia, hemoconcentração e redução nas contagens de plaquetas e leucócitos. Os resultados sinalizam o provável momento do desenvolvimento de lesões do tecido laminar em equinos com síndrome cólica, no qual é possível adotar medidas preventivas contra a laminite.The gastrointestinal diseases of horses are aggravated by complications such as laminitis. The laminitis etiopathogeny are connected with lamellar basement membrane degradation by matrix metalloproteinases (MMPs. Inflammatory cytokines and leukocytes enzymes can active MMPs. The object of this study was to evaluate morphological changes on lamellar tissue of horses with colic syndrome and its association with clinical and laboratorial parameters. It was observed intensive destruction of lamellar architecture, mainly on animals with severe physical and laboratorial alterations, such as delayed capillary refill time, congested mucous membrane, tachycardia, hemoconcentration and low count of platelet and leukocytes. The results sign to the most likely moment of development of lamellar tissue injuries in horses with colic syndrome, which can be adopted preventive measures against laminitis.

  19. Traitement De La Peripneumonie Contagieuse Bovine Par L ...

    African Journals Online (AJOL)

    Traitement De La Peripneumonie Contagieuse Bovine Par L'oxyteTracycline Longe Action Et Transmission Experimentale de la Maladie A Partir de Bovins Traites. ... Tous les 14 animaux ont séroconverti et l'analyse post-mortem a montré la présence des lésions chroniques dont des séquestres pulmonaires chez 4 d'entre ...

  20. Recherche documentaire par titrage automatique

    OpenAIRE

    Lopez , Cédric; Prince , Violaine; Roche , Mathieu

    2011-01-01

    Nous proposons dans cet article un système facilitant la recherche d'information dans un ensemble de documents textuels, basé sur le titrage (et sous-titrage) automatique. Ce dernier peut se révéler crucial, par exemple, dans le cadre de la problématique de l'accessibilité des pages web (norme W3C). Notre processus de titrage automatique consiste à extraire des syntagmes nominaux pertinents dans les textes, pouvant constituer des titres ou sous-titres candidats. Une approche originale combina...

  1. Measurement of radioactive isotopes by {gamma} and x rays spectrometry with INa crystals. application to radiochemistry of some fission and activation products; Mesures d'isotopes radioactifs par spectrometrie {gamma} et x a l'aide de cristaux INa. application a la radiochimie de certains produits de fission et d'activation

    Energy Technology Data Exchange (ETDEWEB)

    Simonet, P; Boile, G; Simonet, G [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

    1965-07-01

    The measurement of an number of atoms N may be obtained by detection of one {gamma} ray emitted if its branching ratio K{sub E} is known (number of E energy {gamma} transitions/number of disintegrations). N = A/{lambda} N{sub {gamma}}{sub E} / (K{sub E} * {lambda}) This measurement is effectuated in well-defined geometry, {gamma} rays emitted by the source are detected by a calibrated NaI crystal which is connected to a photomultiplier delivering electric impulses analysed by a multichannel analyser. Crystals are chosen according to energy, intensity of measured rays and of background. Calibration is established with standard sources or by total absolute efficiency of a definite crystal. Half-life, energy of measured photopeak branching ratio have been determined for these isotopes: Fission products: {sup 95}Zr, {sup 95}Nb, {sup 99}Mo, {sup 103}Ru, {sup 106}Ru, {sup 111}Ag, {sup 115}Cd, {sup 115m}Cd, {sup 132}Te, {sup 129m}Te, {sup 134}Cs, {sup 136}Cs, {sup 137}Cs, {sup 140}Ba, {sup 140}La. Rare earth elements: {sup 91}Y, {sup 141}Ce, {sup 144}Ce, {sup 147}Nd, {sup 156}Eu. Products from reactions (n, {gamma}) (n, 2n): 1{sup 10}Ag, {sup 124}Sb, {sup 239}Np, {sup 237}U, {sup 241}Am. (authors) [French] La mesure du nombre d'atomes N d'un isotope peut s'effectuer sur un rayonnement {gamma} si l'on connait le rapport d'embranchement K{sub E} (nombre de rayonnements {gamma} d'energie E/nombre de desintegrations). N = A/{lambda} N{sub {gamma}}{sub E} / (K{sub E} * {lambda}) La mesure s'effectue en geometrie definie; les rayonnements {gamma} emis par la source sous mylar sont detectes par un cristal INa etalonne en fonction de l'energie, lequel est relie a un photomultiplicateur delivrant des impulsions electriques qui seront analysees par un selecteur multicanaux. La connaissance de la distribution theorique (effet photoelectrique, compton, paires, retrodiffusion, echappement... ) permet de determiner qualitativement si les elements sont purs, et de faire une mesure sur

  2. Association between clinical parameters and the presence of active caries lesions in first permanent molars Associação entre parâmetros clínicos e a presença de lesões ativas de cárie em primeiros molares permanentes

    Directory of Open Access Journals (Sweden)

    Juliana Maria Quaglio

    2006-12-01

    Full Text Available The aim of the present study was to evaluate the association between clinical parameters and the presence of active caries lesions on the occlusal surface of first permanent molars. Forty eight children (5.8-13.8 years-old with at least one first permanent molar present were selected. The clinical parameters evaluated were gender, age, DMF-T and dmf-t, presence of active white spots in other teeth, general plaque index, tooth's dental arch (upper or lower, tooth's side (right or left, presence of visible plaque and eruption degree of the first permanent molars. The first permanent molars were evaluated through visual inspection by two examiners in order to assess the presence of active or inactive caries lesions on the occlusal surface. Univariate and multivariate analyses for determination of the association between clinical parameters and the presence of active caries lesions in these teeth were performed. The presence of active white spots in other teeth was associated with the presence of active caries lesions in the first permanent molars, in both univariate and multivariate analyses (Odds ratio = 8.8 and 1.9, respectively. The presence of abundant visible plaque on the occlusal surface of the first permanent molars (Odds ratio = 3.5 in the univariate analysis, and 3.9 in the multivariate one also presented a significant association. In conclusion, the presence of active white spots in other teeth and the presence of considerable visible plaque were associated with the presence of active caries lesions on the occlusal surfaces of first permanent molars.O objetivo do presente trabalho foi avaliar a associação entre parâmetros clínicos e presença de lesões ativas de cárie sobre a superfície oclusal de primeiros molares permanentes. Quarenta e oito crianças (5,8-13,8 anos com pelo menos um primeiro molar permanente foram selecionadas. Os parâmetros clínicos avaliados foram sexo, idade, CPO-D e ceo-d, presença de manchas brancas

  3. Automation on computer of the partial area method in the analysis of resonances induced by 'S' neutrons 2. with an interference term and extension of the method to the treatment of multi resonances (1963); Automatisation sur ordinateur de la methode des aires partielles dans l'analyse des resonances induites par les neutrons ''S''. 2, avec terme d'interference et extension de la methode au traitement des multiresonances (1963)

    Energy Technology Data Exchange (ETDEWEB)

    Bianchi, G; Corge, C R [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

    1963-07-01

    This report deals with the numerical analysis on an I.B.M. 7090 computer of transmission resonances induced by 's' wave neutrons in time of flight experiments. The analysis method used is the partial area one. In this second part the interference term is taken into account. Modifications have been made in the programs and subroutines described in the first part, to determine the resonant transmissions from experimental raw data, and the relating partial areas. Also programs and subroutines are thoroughly described, which estimate the resonance parameters. The field of the partial area method has been extended to cover the case where several resonances have to be treated simultaneously, provided they do not interfere. (authors) [French] Le pretent rapport a pour objet l'analyse numerique sur ordinateur I.B.M. 7090 des resonances dues aux neutrons ''s'' dans les experiences de transmission par temps de vol, la methode d'analyse utilisee etant la methode dea aires partielles. Dans cette deuxieme partie il a ete tenu compte du terme d'interference. On y trouvera une description des amenagements apportes aux programmes et sous-programmes decrits dans la premiere partie pour determiner les transmissions interfero-resonnantes a partir des donnees experimentales brutes et les aires partielles afferentes. Sont egalement decrits les programmes et sous-programmes necessaires au calcul des parametres caracteristiques des resonances. Le domaine d'application de la methode a ete etendu au traitement simultane de plusieurs resonances groupees n'interferant pas entre elles. (auteurs)

  4. SECHAGE DE PRODUITS GRANULAIRES PAR DESHYDRATATION PAR DETENTES SUCCESSIVES (DDS)

    OpenAIRE

    Abdulla , Galal; Mellouk , Hamid; Belghit , Abdelhamid; Allaf , Karim

    2009-01-01

    International audience; Dans cette étude, nous avons évalué les performances d'un nouveau procédé de séchage dans le cas du liège en granulés. Ce procédé (La déshydratation par détentes successives : DDS) consiste à soumettre le matériau liège dans une chambre hermétique aux variations cycliques de la pression. Chaque cycle est composé d'une compression et d'une décompression. La phase de compression est réalisée en injectant de l'air comprimé à température ambiante provenant d'un compresseur...

  5. Arthrose médiale du genou : évaluations et recommandations cliniques pour les traitements par infiltration, orthèses plantaires et/ou orthèse du genou à l'aide d'analyses quantifiées de la marche

    OpenAIRE

    Dessery, Yoann

    2014-01-01

    L’arthrose du genou (gonarthrose) est une des sources d’incapacité physique les plus fréquentes dans le monde. Plusieurs traitements sont disponibles pour réduire la douleur et ralentir l’évolution de la maladie. Cependant, certains de ces traitements manquent encore de documentation ou d’ajustement par rapport aux patients. L’objectif général de cette thèse est d’évaluer l’impact de différents traitements de la gonarthrose médiale sur la vie quotidienne et le patron de marche afin d’aider le...

  6. La preuve par le nombre

    Directory of Open Access Journals (Sweden)

    Hélène Verdier

    2012-04-01

    Full Text Available S’il en était besoin, la question des usages et de la finalité des résultats de l’inventaire général pourrait être abordée par le nombre : nombre d’œuvres répertoriées, de communes inventoriées, de clichés photographiques réalisés, de dossiers, de consultations des notices dans les bases de données… Les additions aujourd’hui sont éloquentes, mais elles laissent dans l’ombre le rôle de l’inventaire qui, en mettant en œuvre une démarche systématique de connaissance, participe à l’ identificatio...

  7. Serum tetranectin is an independent prognostic marker in colorectal cancer and weakly correlated with plasma suPAR, plasma PAI-1 and serum CEA

    DEFF Research Database (Denmark)

    Høgdall, Claus K; Christensen, Ib J; Stephens, Ross W

    2002-01-01

    PAR and CEA had a 2.43 increased risk as compared to a patient with median levels of these biochemical markers. Significant correlations were found with Dukes' stages for all the biochemical markers and between the respective biochemical markers. The findings confirm that TN is a strong prognostic factor...... activator (uPAR) and carcinoembryonic antigen (CEA). Significantly shorter survival was found for patients with TN levels below a cut-off point of 7.5 mg/l compared to patients with levels above, as illustrated by Kaplan-Meier curves. By Cox analyses, log TN, log soluble uPAR as well as log CEA were found...... to have an independent prognostic value for survival (log TN: HR = 0.47, 95% CI: 0.29-0.76); log soluble uPAR: HR = 1.65, 95% CI: 1.18-2.31; log CEA: HR = 1.I1, 95% CI: 1.03-1.20). Based on the multivariate model, a patient with a combination of low levels of TN and PAI-1 and elevated levels of soluble u...

  8. Par Pond vegetation status Summer 1995 -- Summary

    International Nuclear Information System (INIS)

    Mackey, H.E. Jr.; Riley, R.S.

    1996-01-01

    The water level of Par Pond was lowered approximately 20 feet in mid-1991 in order to protect downstream residents from possible dam failure suggested by subsidence on the downstream slope of the dam and to repair the dam. This lowering exposed both emergent and nonemergent macrophyte beds to drying conditions resulting in extensive losses. A survey of the newly emergent, shoreline aquatic plant communities of Par Pond began in June 1995, three months after the refilling of Par Pond to approximately 200 feet above mean sea level. These surveys continued in July, September, and late October, 1995. Communities similar to the pre-drawdown, Par Pond aquatic plant communities are becoming re-established. Emergent beds of maidencane, lotus, waterlily, and watershield are extensive and well developed. Cattail occurrence continued to increase during the summer, but large beds common to Par Pond prior to the drawdown have not formed. Estimates from SPOT HRV, remote sensing satellite data indicated that as much as 120 hectares of emergent wetlands vegetation may have been present along the Par Pond shoreline by early October, 1995. To track the continued development of macrophytes in Par Pond, future surveys throughout 1996 and 1997, along with the continued evaluation of satellite data to map the areal extent of the macrophyte beds of Par Pond, are planned

  9. Effects of solar PAR and UV radiation on tropical biofouling communities

    KAUST Repository

    Dobretsov, SV; Gosselin, L; Qian, P

    2010-01-01

    We investigated the effect of solar ultraviolet radiation (UVR) and photosynthetically active radiation (PAR) on the development of tropical micro- and macrofouling communities for 30 d. The experimental design involved 3 treatments: full spectrum

  10. L’aggressività della coppia criminale: la strage di Erba analizzata nell’ottica della coscienza intersoggettiva di D. Stern/L'agressivité des couples criminels : le massacre de Erba analysé par l'approche de la conscience intersubjective de Daniel Stern/The agressiveness of the criminal couples: the analysed massacre of Erba using the approach of the intersubjective conscience of Daniel Stern

    Directory of Open Access Journals (Sweden)

    Pasquale Caponnetto

    2013-12-01

    Full Text Available La coppia criminale è un fenomeno poco comune nell'ambito dei delitti in quanto la maggior parte dei criminali commette il crimine da solo o coinvolge altri individui solo per caso. Ciò nonostante, la letteratura rivela una serie di coppie criminali che hanno commesso diversi crimini, fino ad arrivare all’omicidio. Alcuni hanno ucciso per denaro, altri per odio, altri ancora per amore, per vendetta, per perversione. Bonnie e Clyde, Olindo e Rosa, Erika e Omar sono espressione di un viaggio nel fenomeno del “crimine di coppia” in cui manifestazioni di follia a due, al di là dell’infatuazione reciproca e della dipendenza psicologica, si svuotano di qualsiasi accezione romantica per concretizzarsi in qualcosa di spaventoso e incontenibile, capace di sfuggire ad ogni tipo di catalogazione e comprensione. Nel presente lavoro, in particolare, si andrà ad analizzare la coppia criminale degli autori della strage di Erba, che verrà esaminata nell’ottica della coscienza intersoggettiva di Stern. Le couple criminel est un phénomène rare puisque la majorité des crimes sont commis par des criminels seuls ou ce n’est que par hasard qu’ils impliquent d’autres personnes. Toutefois, la littérature parle d’une série de couples criminels qui ont commis des différents types de crimes (homicide inclus. Certains d’entre eux tuent pour de l’argent, certains autres à cause de la haine, de l’amour, de la revanche ou de la perversion. Bonnie et Clyde, Olindo et Rosa, Erika et Omar constituent des exemples du phénomène du « crime en couple ». Ce sont des situations de folie à deux qui n’ont aucune acception romantique et qui se traduisent en quelque chose d’horrifiant et d’incontrôlé. Dans cet article, les auteurs vont analyser le couple criminel composé par les responsables du massacre de Erba (Italie du Nord. L’objectif est d’examiner ce couple en utilisant l’approche de la conscience intersubjective de Daniel

  11. Usefulness of suPAR as a biological marker in patients with systemic inflammation or infection: a systematic review

    NARCIS (Netherlands)

    Backes, Yara; van der Sluijs, Koenraad F.; Mackie, David P.; Tacke, Frank; Koch, Alexander; Tenhunen, Jyrki J.; Schultz, Marcus J.

    2012-01-01

    Systemic levels of soluble urokinase-type plasminogen activator receptor (suPAR) positively correlate with the activation level of the immune system. We reviewed the usefulness of systemic levels of suPAR in the care of critically ill patients with sepsis, SIRS, and bacteremia, focusing on its

  12. Development of Mesorhizobium ciceri-Based Biofilms and Analyses of Their Antifungal and Plant Growth Promoting Activity in Chickpea Challenged by Fusarium Wilt.

    Science.gov (United States)

    Das, Krishnashis; Rajawat, Mahendra Vikram Singh; Saxena, Anil Kumar; Prasanna, Radha

    2017-03-01

    Biofilmed biofertilizers have emerged as a new improved inoculant technology to provide efficient nutrient and pest management and sustain soil fertility. In this investigation, development of a Trichoderma viride - Mesorhizobium ciceri biofilmed inoculant was undertaken, which we hypothesized, would possess more effective biological nitrogen fixing ability and plant growth promoting properties. As a novel attempt, we selected Mesorhizobium ciceri spp. with good antifungal attributes with the assumption that such inoculants could also serve as biocontrol agents. These biofilms exhibited significant enhancement in several plant growth promoting attributes, including 13-21 % increase in seed germination, production of ammonia, IAA and more than onefold to twofold enhancement in phosphate solubilisation, when compared to their individual partners. Enhancement of 10-11 % in antifungal activity against Fusarium oxysporum f. sp. ciceri was also recorded, over the respective M. ciceri counterparts. The effect of biofilms and the M. ciceri cultures individual on growth parameters of chickpea under pathogen challenged soil illustrated that the biofilms performed at par with the M. ciceri strains for most plant biometrical and disease related attributes. Elicitation of defense related enzymes like l-phenylalanine ammonia lyase, peroxidase and polyphenol oxidase was higher in M. ciceri /biofilm treated plants as compared to uninoculated plants under pathogen challenged soil. Further work on the signalling mechanisms among the partners and their tripartite interactions with host plant is envisaged in future studies.

  13. Contribution au développement d'un procédé de stabilisation d'une boisson à base d'eau et de sirop d'érable par la technologie d'électro-activation en solution

    OpenAIRE

    Koffi, Kouassi

    2013-01-01

    Le développement de nouveaux moyens pour traiter les denrées et produits agroalimentaires par l'utilisation des techniques électrochimiques a déjà joué un rôle important dans nombres de procédés. Parmi ces techniques électrochimiques, l’électro-activation, constitue à ce jour une voie nouvelle pour le traitement et l’amélioration de la qualité de produits et de solutions aqueuses en industrie agroalimentaire. L’électro-activation est dite «reagentless technology», c’est-à-dire une technologie...

  14. Identification of a new epitope in uPAR as a target for the cancer therapeutic monoclonal antibody ATN-658, a structural homolog of the uPAR binding integrin CD11b (αM.

    Directory of Open Access Journals (Sweden)

    Xiang Xu

    Full Text Available The urokinase plasminogen activator receptor (uPAR plays a role in tumor progression and has been proposed as a target for the treatment of cancer. We recently described the development of a novel humanized monoclonal antibody that targets uPAR and has anti-tumor activity in multiple xenograft animal tumor models. This antibody, ATN-658, does not inhibit ligand binding (i.e. uPA and vitronectin to uPAR and its mechanism of action remains unclear. As a first step in understanding the anti-tumor activity of ATN-658, we set out to identify the epitope on uPAR to which ATN-658 binds. Guided by comparisons between primate and human uPAR, epitope mapping studies were performed using several orthogonal techniques. Systematic site directed and alanine scanning mutagenesis identified the region of aa 268-275 of uPAR as the epitope for ATN-658. No known function has previously been attributed to this epitope Structural insights into epitope recognition were obtained from structural studies of the Fab fragment of ATN-658 bound to uPAR. The structure shows that the ATN-658 binds to the DIII domain of uPAR, close to the C-terminus of the receptor, corroborating the epitope mapping results. Intriguingly, when bound to uPAR, the complementarity determining region (CDR regions of ATN-658 closely mimic the binding regions of the integrin CD11b (αM, a previously identified uPAR ligand thought to be involved in leukocyte rolling, migration and complement fixation with no known role in tumor progression of solid tumors. These studies reveal a new functional epitope on uPAR involved in tumor progression and demonstrate a previously unrecognized strategy for the therapeutic targeting of uPAR.

  15. Activation of PAR-1/NADPH oxidase/ROS signaling pathways is crucial for the thrombin-induced sFlt-1 production in extravillous trophoblasts: possible involvement in the pathogenesis of preeclampsia.

    Science.gov (United States)

    Huang, Qi-Tao; Chen, Jian-Hong; Hang, Li-Lin; Liu, Shi-San; Zhong, Mei

    2015-01-01

    Preeclampsia was characterized by excessive thrombin generation in placentas and previous researches showed that thrombin could enhance soluble Fms-like tyrosine kinase 1 (sFlt-1) expression in first trimester trophoblasts. However, the detailed mechanism for the sFlt-1 over-production induced by thrombin was largely unknown. The purpose of this study was to explore the possible signaling pathway of thrombin-induced sFlt-1 production in extravillous trophoblasts (EVT). An EVT cell line (HRT-8/SVneo) was treated with various concentrations of thrombin. The mRNA expression and protein secretion of sFlt-1 in EVT were detected with real-time polymerase chain reaction and ELISA, respectively. The levels of intracellular reactive oxygen species (ROS) production were determined by DCFH-DA. Exposure of EVT to thrombin induced increased intracellular ROS generation and overexpression of sFlt-1 at both mRNA and protein levels in a dose dependent manner. Short interfering RNA (siRNA) directed against PAR-1 or apocynin (an inhibitor of NADPH oxidase) could decrease the intracellular ROS generation and subsequently suppressed the production of sFlt-1 at mRNA and protein levels. Our results suggested that thrombin increased sFlt-1 production in EVT via the PAR-1 /NADPH oxidase /ROS signaling pathway. This also highlights the PAR-1 / NADPH oxidase / ROS pathway might be a potential therapeutic target for the prevention of preeclampsia in the future. © 2015 S. Karger AG, Basel.

  16. MODIS-derived daily PAR simulation from cloud-free images and its validation

    Energy Technology Data Exchange (ETDEWEB)

    Chen, Liangfu; Gu, Xingfa; Tian, Guoliang [State Key Laboratory of Remote Sensing Science, Jointly Sponsored by Institute of Remote Sensing Applications of Chinese Academy of Sciences and Beijing Normal University, Beijing 100101 (China); The Center for National Spaceborne Demonstration, Beijing 100101 (China); Gao, Yanhua [State Key Laboratory of Remote Sensing Science, Jointly Sponsored by Institute of Remote Sensing Applications of Chinese Academy of Sciences and Beijing Normal University, Beijing 100101 (China); Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101 (China); Yang, Lei [State Key Laboratory of Remote Sensing Science, Jointly Sponsored by Institute of Remote Sensing Applications of Chinese Academy of Sciences and Beijing Normal University, Beijing 100101 (China); Jilin University, Changchun 130026 (China); Liu, Qinhuo [State Key Laboratory of Remote Sensing Science, Jointly Sponsored by Institute of Remote Sensing Applications of Chinese Academy of Sciences and Beijing Normal University, Beijing 100101 (China)

    2008-06-15

    In this paper, a MODIS-derived daily PAR (photosynthetically active radiation) simulation model from cloud-free image over land surface has been developed based on Bird and Riordan's model. In this model, the total downwelling spectral surface irradiance is divided into two parts: one is beam irradiance, and another is diffuse irradiance. The attenuation of solar beam irradiance comprises scattering by the gas mixture, absorption by ozone, the gas mixture and water vapor, and scattering and absorption by aerosols. The diffuse irradiance is scattered out of the direct beam and towards the surface. The multiple ground-air interactions have been taken into account in the diffuse irradiance model. The parameters needed in this model are atmospheric water vapor content, aerosol optical thickness and spectral albedo ranging from 400 nm to 700 nm. They are all retrieved from MODIS data. Then, the instantaneous photosynthetically available radiation (IPAR) is integrated by using a weighted sum at each of the visible MODIS wavebands. Finally, a daily PAR is derived by integration of IPAR. In order to validate the MODIS-derived PAR model, we compared the field PAR measurements in 2003 and 2004 against the simulated PAR. The measurements were made at the Qianyanzhou ecological experimental station, Chinese Ecosystem Research Network. A total of 54 days of cloud-free MODIS L1B level images were used for the PAR simulation. Our results show that the simulated PAR is consistent with field measurements, where the correlation coefficient of linear regression between calculated PAR and measured PAR is 0.93396. However, there were some uncertainties in the comparison of 1 km pixel PAR with the tower flux stand measurement. (author)

  17. Correcting the effects of the matrix using capture gamma-ray spectrometry: Application to measurement by Active Neutron Interrogation; Correction des effets de matrice par spectrometrie des rayonnements gamma de capture: Application a la mesure par Interrogation Neutronique Active (I.N.A.)

    Energy Technology Data Exchange (ETDEWEB)

    Baudry, G.

    2003-11-15

    In the field of the measurement of low masses of fissile material ({sup 235}U, {sup 239}Pu, {sup 241}Pu) in radioactive waste drums, the Active Neutron Interrogation is a non-destructive method achieving good results. It does however remain reliant upon uncertainties related to the matrix effects on interrogation and fission neutrons. The aim of this thesis is to develop a correction method able to take into account these matrix effects by quantifying the amount of absorbent materials (chlorine and hydrogen) in a 118- liter homogeneous matrix. The main idea is to use the gamma-ray spectrometry of gamma emitted by neutron captures to identify and quantify the composition of the matrix. An indicator from its chlorine content is then deduced in order to choose the calibration coefficient which best represents the real composition of the matrix. This document firstly presents the needs of control and characterization of radioactive objects, and the means used in the field of nuclear measurement. Emphases is put in particular on the Active Neutron Interrogation method. The matrices of interest are those made of light technological waste (density {<=} 0,4 g/cm{sup 3}) containing hydrogenated and chlorinated materials. The advantages of gamma-rays emitted by neutron captures for the determination of a chlorine content indicator of the matrices and the principles of the correction method are then explained. Measurements have been firstly realized with an existing Neutron Interrogation device (PROMETHEE 6). Such measurements have proven its inadequacy: no signal from the matrix hydrogen was detected, due to an intense signal from the polyethylene contained in some cell elements. Moreover, the matrix chlorine content appeared difficult to be measured. A new and specific device, named REGAIN and dedicated to active gamma-rays spectrometry, was defined with the Monte-Carlo N-Particle (MCNP) code. The experiments conducted with this new device made it possible to detect the

  18. ¡París!

    Directory of Open Access Journals (Sweden)

    Beatriz Caballero

    2003-01-01

    Full Text Available El día no le alcanzaba para caminarlo. Conocía cada puente, cada calle,plaza, museo, rincón, iglesia, café, y la mesa con el mejor ángulo para mirar siempre una iglesia, ojalá Saint-Germain-des-Pres, o Notre-Dame. Siempre contaba la impresión que le había causado a Bolívar ver allí cuando el papa coronó a Napoleón. Él se iba para la oficina todos los días, Luis a un taller a pintar, Antonio a estudiar ciencias políticas, yo al colegio y mamá hacía las cosas prácticas. Aprendíamos francés todos al tiempo y por la noche nos contábamos lo nuevo que cada uno había aprendido. Mamá después, cuando ya se quería volver, decía que París nos había maleado a todos. Estaba en pleno furor el cine francés de la "nueva ola", pero ella sólo me llevaba a ver películas de vaqueros pues no se fiaba de la clasificación de los periódicos.

  19. Increased plasma soluble uPAR level is a risk marker of respiratory cancer in initially cancer-free individuals

    DEFF Research Database (Denmark)

    Langkilde, Anne A; Hansen, Tine Willum; Ladelund, Steen

    2011-01-01

    BACKGROUND: Soluble urokinase plasminogen activator receptor (suPAR) is a stable plasma biomarker associated with inflammation and disease. This study tested the association between suPAR levels and incident respiratory, gastrointestinal or other types of cancer in initially cancer-free individuals...... with respiratory, gastrointestinal and other cancer types, respectively.CONCLUSIONS: Elevated suPAR levels were associated with increased risk of incident respiratory cancer and other types of cancer, but not gastrointestinal cancers, independently of established risk factors, CRP and leukocyte numbers. Impact.......RESULTS: suPAR levels ranged from 0.6-22 ng/ml, and median suPAR level was 4.01 ng/ml. 1 ng/ml increase in baseline suPAR was associated with adjusted hazard ratios (HR) of 1.61 (95% CI: 1.23-2.11, P

  20. Outdoor recreation activity trends by volume segments: U.S. and Northeast market analyses, 1982-1989

    Science.gov (United States)

    Rodney B. Warnick

    1992-01-01

    The purpose of this review was to examine volume segmentation within three selected outdoor recreational activities -- swimming, hunting and downhill skiing over an eight-year period, from 1982 through 1989 at the national level and within the Northeast Region of the U.S.; and to determine if trend patterns existed within any of these activities when the market size...

  1. Prognostic value of suPAR and hs-CRP on cardiovascular disease

    DEFF Research Database (Denmark)

    Diederichsen, Marie Zöga; Diederichsen, Søren Zöga; Mickley, Hans

    2018-01-01

    Background and aims: Studies have shown that soluble urokinase Plasminogen Activator Receptor (suPAR) and CRP (both inflammatory markers) and coronary artery calcification (CAC) are independent risk predictors for cardiovascular (CV) disease. The aim of this study is to assess whether suPAR and CRP...... have an increased predictive prognostic value beyond the traditional CV risk factors and the CAC score. Methods: A population sample of 1179 subjects, free of CV disease was included. The subjects underwent traditional CV risk evaluation, CAC assessment and blood sampling for suPAR and CRP. CV events...

  2. Thrombin induces rapid PAR1-mediated non-classical FGF1 release

    International Nuclear Information System (INIS)

    Duarte, Maria; Kolev, Vihren; Soldi, Raffaella; Kirov, Alexander; Graziani, Irene; Oliveira, Silvia Marta; Kacer, Doreen; Friesel, Robert; Maciag, Thomas; Prudovsky, Igor

    2006-01-01

    Thrombin induces cell proliferation and migration during vascular injury. We report that thrombin rapidly stimulated expression and release of the pro-angiogenic polypeptide fibroblast growth factor 1 (FGF1). Thrombin failed to induce FGF1 release from protease-activated receptor 1 (PAR1) null fibroblasts, indicating that this effect was dependent on PAR1. Similarly to thrombin, FGF1 expression and release were induced by TRAP, a specific oligopeptide agonist of PAR1. These results identify a novel aspect of the crosstalk between FGF and thrombin signaling pathways which both play important roles in tissue repair and angiogenesis

  3. Avaliação da cristalização de mel utilizando parâmetros de cor e atividade de água Evaluation of honey crystallization from the colour and water activity parameters

    Directory of Open Access Journals (Sweden)

    Alini Mari Kuroishi

    2012-03-01

    Full Text Available O mel é um dos edulcorantes mais antigos utilizados pelo homem, sendo composto principalmente de monossacarídeos, como a glicose e a frutose, responsáveis em parte pela sua cristalização. A cristalização varia segundo diversos fatores, como a concentração de açúcares, o teor de água na sua composição natural, a procedência floral do néctar, o manuseio durante seu processamento, bem como as condições de estocagem. O presente trabalho teve como objetivo avaliar o efeito da temperatura e da incorporação de cristais de mel na cristalização do mel utilizando como parâmetro de acompanhamento a cor, por meio da determinação do Índice de Brancura (IB e variação da luminosidade (∆L*, e a atividade de água. Utilizou-se um delineamento experimental fatorial 2², tendo-se como variáveis o percentual de cristais de mel adicionado e a temperatura de armazenamento. Apenas as amostras armazenadas à temperatura de 11 e 21 °C cristalizaram, sendo mais evidente a cristalização da amostra armazenada em 11 °C, que foi adicionada de 5% de cristais de mel. O aumento da atividade de água e o aumento no Índice de Brancura (IB, assim como o ∆L*, foram correlacionados com a cristalização das amostras. A maior variação obtida para os valores de ∆L* encontram-se na região de temperatura de armazenamento de 11 °C, na qual as amostras cristalizaram mais rapidamente.Honey is one of the oldest sweeteners used by man, composed mainly of monosaccharides such as glucose and fructose, partially responsible for its crystallization. Honey crystallization depends on several factors, such as the sugar concentrations, natural percentage of water, the floral origin of the nectar, handling during processing and the storage conditions. The present work aimed to evaluate the effect of temperature and of the incorporation of honey crystals on honey crystallization, using the colour (as determined by the Whiteness Index - WI and changes in the

  4. New developments in the analysis and measurement of thicknesses by {beta}-particle excitation of X fluorescent rays; Nouveaux developpements de l'analyse et de la mesure des epaisseurs par excitation des raies de fluorescence X au moyen de particules {beta}; Novye usovershenstvovaniya analiza i izmereniya plotnosti putem vozbuzhdeniya fluorestsiruyushchikh spektrov rentgenovskogo lucha beta-chastitsami; Nuevos adelantos en el analisis y la medicion de espesores mediante la excitacion de rayos X por particulas beta

    Energy Technology Data Exchange (ETDEWEB)

    Martinelli, P [Commissariat a l' Energie Atomique, Saclay (France); Seibel, G [Institut de Recherches de la Siderurgie, St-Germain-en-Laye (France)

    1962-01-15

    The method of analysing and measuring the thickness of deposits by {beta}-X fluorescence which we previously described has been further developed. Using Pm{sup 147} and Kr{sup 85} sources, it is possible to reduce the background observed with Sr{sup 90}. We quote the results obtained for various thickness measurements of metal deposits, an analysis of the solutions, and the continuous measurement of calcium and iron in core samples. We describe experiments made for analysis of the X-radiation by crystal. (author) [French] Le procede d'analyse et de mesure des epaisseurs de depots par fluorescence {beta}-X que nous avons precedemment decrit a fait l'objet de nouveaux developpements. L'emploi de sources de {sup 147}Pm et de {sup 85}Kr permet de reduire le bruit de fond que l'on observe avec le {sup 90}Sr. Nous donnons les resultats obtenus pour diverses mesures d'epaisseurs de depots metalliques, l'analyse des solutions et la mesure en continu du calcium et du fer dans les carottes de minerais. Nous decrivons les essais effectues en vue d'analyser le rayonnement X au moyen d'un cristal. (author) [Spanish] Los autores han introducido nuevos perfeccionamientos en su procedimiento de analisis y de medicion de espesores de depositos por fluorescencia de rayos X excitada por particulas beta. La utilizacion de fuentes de {sup 147}Pm y de {sup 85}Kr permite reducir la actividad de fondo que se observa empleando {sup 90}Sr. Los autores exponen los resultados obtenidos en materia de mediciones de espesores de depositos metalicos, analisis de soluciones y medicion continua del calcio y del hierro en muestras de minerales extraidas por sondeo. Tambien describen los ensayos realizados con miras a analizar los rayos X por medio de un cristal. (author) [Russian] Protsess analiza i izmereniya plotnosti sloev putem fluorestsiruyushchikh beta- i rentgenovskikh luchej, kotoryj opisyvalsya nami ran'she, byl predmetom novykh usovershenstvovanij. Ispol'zovanie istochnikov prometeya-147 i

  5. The effect of online chronic disease personas on activation: within-subjects and between-groups analyses.

    Science.gov (United States)

    Serio, Catherine Devany; Hessing, Jason; Reed, Becky; Hess, Christopher; Reis, Janet

    2015-02-25

    Although self-management of chronic disease is important, engaging patients and increasing activation for self-care using online tools has proven difficult. Designing more tailored interventions through the application of condition-specific personas may be a way to increase engagement and patient activation. Personas are developed from extensive interviews with patients about their shared values and assumptions about their health. The resulting personas tailor the knowledge and skills necessary for self-care and guide selection of the self-management tools for a particular audience. Pre-post changes in self-reported levels of activation for self-management were analyzed for 11 chronic health personas developed for 4 prevalent chronic diseases. Personas were created from 20 to 25 hour-long nondirected interviews with consumers with a common, chronic disease (eg, diabetes). The interviews were transcribed and coded for behaviors, feelings, and beliefs using the principles of grounded theory. A second group of 398 adults with self-reported chronic disease were recruited for online testing of the personas and their impact on activation. The activation variables, based on an integrated theory of health behavior, were knowledge of a given health issue, perceived self-management skills, confidence in improving health, and intention to take action in managing health. Pre-post changes in activation were analyzed with a mixed design with 1 within-subjects factor (pre-post) and 1 between-group factor (persona) using a general linear model with repeated measures. Sixteen pre-post changes for 4 measures of activation were analyzed. All but 2 of the within-subjects effects were statistically significant and all changes were in the direction of increased activation scores at posttest. Five significant differences between personas were observed, showing which personas performed better. Of low activation participants, 50% or more shifted to high activation across the 4 measures

  6. Survey of awareness and analyses of related factors to volunteer activities of pharmacy students after the Great East Japan Earthquake

    OpenAIRE

    小武家, 優子; 吉田, 健; 吉武, 毅人

    2012-01-01

    The Great East Japan Earthquake occurred on March 11, 2011. At the time of the earthquake, pharmacist and pharmacy students engaged in volunteer activities such as providing disaster medicine and relief supplies to disaster areas. Questionnaire survey for pharmacy students were carried out in order to clarify awareness to volunteer activities for disaster areas and to use data as a basis of Service-Learning in the 6 years pharmacy education. We divided subjects into pharmacy students those wo...

  7. Analytical analyses of active control of sound transmission through soft-core sandwich structures and double panel partitions

    OpenAIRE

    Sahu, Kiran Chandra

    2016-01-01

    Active structural acoustic control (ASAC) is a form of active noise control which focuses on the control of structural vibrations in a manner that minimizes acoustic radiation from a structure. The greatest difficulty ASAC suffers from is in finding an "optimal" error quantity, which can be easily implemented in a control algorithm. Volume velocity control (VVC) metric which is generally used in ASAC typically requires either a large number of sensors distributed across the entire structure, ...

  8. Determination of micro-quantities of several elements in soil solution by isotope dilution and activation analyses

    International Nuclear Information System (INIS)

    Cho, C.M.; Axmann, H.

    1965-01-01

    Determination of small quantities of plant nutrients in the soil solution of flooded rice soils is a difficult problem. The concentrations of Mn, Fe and P, for example, in some soil solutions are so small that no chemical method gives any accurate result. Neutron activation analysis was reported to give a much lower limit of detectability for several elements, while for elements with low-induced activity after neutron irradiation, substoichiometric isotopic dilution analysis was applied. One of the advantages of neutron activation analysis lies in the fact that simultaneous activation of every inducible element in a sample takes place. This gives an opportunity to determine many elements by one sample preparation and irradiation. This, however, is not a simple task since identification of the activated products and their quantitative estimation becomes very difficult. Certain operations of separation must be carried out before activity measurements. Ion-exchange resin columns and chemical separation following the addition of carriers were successfully used for the determination of many elements after neutron irradiation. These procedures, however, cannot be directly applied to the determination of the elements of agronomic interest. A procedure was developed to determine several elements of agronomic interest. Times of irradiation and cooling, quick separation by ion-exchange columns, together with chemical precipitation for β-emitters of relatively long half-lives, were all combined to get the maximum benefit from neutron activation analysis. For Fe, for which no satisfactory neutron activation analysis has yet been developed, a modified substoichiometric double isotope dilution procedure is applied

  9. Disposal of Low-Activity Liquid Effluents by Dilution; Elimination par Dilution d'Effluents Liquides Faiblement Radioactifs; 0423 0414 0410 041b 0414 ; Eliminacion de Efluentes Liquidos de Baja Radiactividad por Dilucion

    Energy Technology Data Exchange (ETDEWEB)

    Bovard, P.; Candillon, C. [Service de Controle des Radiations et de Genie Radioactif, Commissariat a l' Energie Atomique, Saclay (France)

    1960-07-01

    obtenus Nous avons commence en 1957 une etude evolutive de l'incidence des installations nucleaires. Cette etude embrasse la determination des niveaux - de radioactivite naturelle - de contamination artificielle afin d'en degager la part revenant aux Centres Nucleaires. Des exemples sont cites faisant appel a des zones de reference. La plus grande partie de la radioactivite artificielle semble due aux retombees radioactives; ceci n'est pas surprenant si on examine les quantites d'isotopes radioactifs rejetees jusqu'a present en France. Un certain nombre d'essais de laboratoire ont ete effectues. Ils ont contribue a preciser certains mecanismes, ont accru notre connaissance de la pollution des sites et nous ont permis d'ameliorer nos methodes de prelevement. II. Essais de laboratoire Ils ont porte: 1. sur des 'phenomenes d'adsorption analogues a ceux produits dans les rivieres au cours de la dilution des effluents: fixation sur mineraux tres divises: mica, quartz. Des radioisotopes tels que {sup 90}Sr, {sup 137}Cs, {sup 90}Y semblent se comporter fort differemment: dans les conditions moyennes de rejet, le {sup 90}Sr serait le moins adsorbe. : 2. sur la contamination du sol et des vegetaux par l'emploi de colonnes et de cubes de sol en place. Ceci nous a conduit a standardiser nos prelevements, afin de faciliter les analyses ulterieures et de comparer utilement les resultats. III. Choix du site et modalites des rejets De ce faisceau de resultats et d'etudes encore incomplets, nous essayons de degager quelques principes quant au choix des sites. Le site une fois defini, l'experience acquise permet de tirer le meilleur parti des possibilites naturelles. (author) [Spanish] Con los resultados obtenidos en Francia vigilando la radiactividad en las proximidades de las instalaciones nucleares se pueden extraer determinadas conclusiones acerca de la eleccion de los procedimientos de evacuacion. I. Resultados obtenidos Los autores iniciaron en 1957 un estudio evolutivo de las

  10. Mutational and structural analyses of Caldanaerobius polysaccharolyticus Man5B reveal novel active site residues for family 5 glycoside hydrolases.

    Science.gov (United States)

    Oyama, Takuji; Schmitz, George E; Dodd, Dylan; Han, Yejun; Burnett, Alanna; Nagasawa, Naoko; Mackie, Roderick I; Nakamura, Haruki; Morikawa, Kosuke; Cann, Isaac

    2013-01-01

    CpMan5B is a glycoside hydrolase (GH) family 5 enzyme exhibiting both β-1,4-mannosidic and β-1,4-glucosidic cleavage activities. To provide insight into the amino acid residues that contribute to catalysis and substrate specificity, we solved the structure of CpMan5B at 1.6 Å resolution. The structure revealed several active site residues (Y12, N92 and R196) in CpMan5B that are not present in the active sites of other structurally resolved GH5 enzymes. Residue R196 in GH5 enzymes is thought to be strictly conserved as a histidine that participates in an electron relay network with the catalytic glutamates, but we show that an arginine fulfills a functionally equivalent role and is found at this position in every enzyme in subfamily GH5_36, which includes CpMan5B. Residue N92 is required for full enzymatic activity and forms a novel bridge over the active site that is absent in other family 5 structures. Our data also reveal a role of Y12 in establishing the substrate preference for CpMan5B. Using these molecular determinants as a probe allowed us to identify Man5D from Caldicellulosiruptor bescii as a mannanase with minor endo-glucanase activity.

  11. Mutational and structural analyses of Caldanaerobius polysaccharolyticus Man5B reveal novel active site residues for family 5 glycoside hydrolases.

    Directory of Open Access Journals (Sweden)

    Takuji Oyama

    Full Text Available CpMan5B is a glycoside hydrolase (GH family 5 enzyme exhibiting both β-1,4-mannosidic and β-1,4-glucosidic cleavage activities. To provide insight into the amino acid residues that contribute to catalysis and substrate specificity, we solved the structure of CpMan5B at 1.6 Å resolution. The structure revealed several active site residues (Y12, N92 and R196 in CpMan5B that are not present in the active sites of other structurally resolved GH5 enzymes. Residue R196 in GH5 enzymes is thought to be strictly conserved as a histidine that participates in an electron relay network with the catalytic glutamates, but we show that an arginine fulfills a functionally equivalent role and is found at this position in every enzyme in subfamily GH5_36, which includes CpMan5B. Residue N92 is required for full enzymatic activity and forms a novel bridge over the active site that is absent in other family 5 structures. Our data also reveal a role of Y12 in establishing the substrate preference for CpMan5B. Using these molecular determinants as a probe allowed us to identify Man5D from Caldicellulosiruptor bescii as a mannanase with minor endo-glucanase activity.

  12. PAR-1 and thrombin: the ties that bind the microenvironment to melanoma metastasis.

    Science.gov (United States)

    Zigler, Maya; Kamiya, Takafumi; Brantley, Emily C; Villares, Gabriel J; Bar-Eli, Menashe

    2011-11-01

    Progression of melanoma is dependent on cross-talk between tumor cells and the adjacent microenvironment. The thrombin receptor, protease-activated receptor-1 (PAR-1), plays a key role in exerting this function during melanoma progression. PAR-1 and its activating factors, which are expressed on tumor cells and the surrounding stroma, induce not only coagulation but also cell signaling, which promotes the metastatic phenotype. Several adhesion molecules, cytokines, growth factors, and proteases have recently been identified as downstream targets of PAR-1 and have been shown to modulate interactions between tumor cells and the microenvironment in the process of melanoma growth and metastasis. Inhibiting such interactions by targeting PAR-1 could potentially be a useful therapeutic modality for melanoma patients. ©2011 AACR.

  13. Rapid evaluation of the neutron dose following a criticality accident by measurement of {sup 24}Na activity; Evaluation rapide de la dose de neutrons a la suite d'un accident de criticite par mesure de l'activite de {sup 24}Na

    Energy Technology Data Exchange (ETDEWEB)

    Estournel, R [Centre de Production de Plutonium de Marcoule, Service de Protection contre les Rayonnements, 30 (France); Henry, Ph [Centre de Production de Plutonium de Marcoule, Section Medicale et Sociale, 30 (France); Beau, P; Ergas, A [Commissariat a l' Energie Atomique, Service d' Hygiene Atomique, Dept. de la Protection Sanitaire, Chusclan, (France)

    1966-07-01

    By external measurement of the gamma activity of {sup 24}Na induced in the human organs by a neutron flux during a criticality accident, it is possible to evaluate the personal dose received. Detectors designed for everyday use in health physics can be applied to these measurements, and this is described in the first part of the work. The response of a certain number of induced-activity detectors is presented. The induced activity-dose relationship is studied theoretically in the second part taking into account the neutron spectrum to which the individual has been subjected. The characteristic spectra of three possible types of accident have been used for deducing this relationship. The results obtained show that the method is sufficiently sensitive for present purposes. The accuracy of this method for calculating the dose received during an experiment is discussed. (authors) [French] La mesure par detection externe de l'activite gamma du sodium 24 induit dans l'organisme humain par un flux de neutrons lors d'un accident de criticite rend possible l'evaluation de la dose recue par un individu irradie. L'utilisation de detecteurs d'un emploi courant en radioprotection fait l'objet d'une experimentation qui constitue la premiere partie de cette etude. La reponse d'un certain nombre de detecteurs a une activite induite connue est presentee. La relation dose-activite induite, est etudiee, de maniere theorique, dans la seconde partie, correlativement au spectre des neutrons qui ont atteint l'individu irradie. Les spectres caracteristiques de trois types d'accidents possibles ont ete retenus pour l'etablissement de ces relations. Les resultats obtenus montrent que la methode satisfait avec une sensibilite suffisante au but recherche. La precision avec laquelle on peut ainsi calculer la dose recue au cours d'un accident de criticite est discutee. (auteurs)

  14. Activation of PAR-1/NADPH Oxidase/ROS Signaling Pathways is Crucial for the Thrombin-Induced sFlt-1 Production in Extravillous Trophoblasts: Possible Involvement in the Pathogenesis of Preeclampsia

    Directory of Open Access Journals (Sweden)

    Qi-tao Huang

    2015-03-01

    Full Text Available Backgrounds/Aims: Preeclampsia was characterized by excessive thrombin generation in placentas and previous researches showed that thrombin could enhance soluble Fms-like tyrosine kinase 1 (sFlt-1 expression in first trimester trophoblasts. However, the detailed mechanism for the sFlt-1 over-production induced by thrombin was largely unknown. The purpose of this study was to explore the possible signaling pathway of thrombin-induced sFlt-1 production in extravillous trophoblasts (EVT. Methods: An EVT cell line (HRT-8/SVneo was treated with various concentrations of thrombin. The mRNA expression and protein secretion of sFlt-1 in EVT were detected with real-time polymerase chain reaction and ELISA, respectively. The levels of intracellular reactive oxygen species (ROS production were determined by DCFH-DA. Results: Exposure of EVT to thrombin induced increased intracellular ROS generation and overexpression of sFlt-1 at both mRNA and protein levels in a dose dependent manner. Short interfering RNA (siRNA directed against PAR-1 or apocynin (an inhibitor of NADPH oxidase could decrease the intracellular ROS generation and subsequently suppressed the production of sFlt-1 at mRNA and protein levels. Conclusions: Our results suggested that thrombin increased sFlt-1 production in EVT via the PAR-1 /NADPH oxidase /ROS signaling pathway. This also highlights the PAR-1 / NADPH oxidase / ROS pathway might be a potential therapeutic target for the prevention of preeclampsia in the future.

  15. Effects of solar PAR and UV radiation on tropical biofouling communities

    KAUST Repository

    Dobretsov, SV

    2010-03-08

    We investigated the effect of solar ultraviolet radiation (UVR) and photosynthetically active radiation (PAR) on the development of tropical micro- and macrofouling communities for 30 d. The experimental design involved 3 treatments: full spectrum (PAR+UVR), PAR only, and minimal light (reduced PAR and UVR). Terminal restriction fragment length polymorphism analysis demonstrated that different light conditions resulted in the formation of highly different microbial communities. The lowest densities of bacteria were found under the full spectrum treatment, while the lowest densities of diatoms were found in the minimal light treatment. Macrofouling communities consisted of 13 species and differed among light treatments. In the presence of UVR, communities had low species diversity, evenness, and richness, while in minimal light and PAR treatments, communities had high species diversity, evenness, and richness. Similarity percentage (SIMPER) analysis revealed that the tubeworm Hydroides elegans, the alga Ulva (Enteromorpha) sp., and the bivalve Perna viridis were the species responsible for most of the dissimilarities in macrofouling communities among treatments. While densities of H. elegans were similar in the PAR and minimal light treatments, this polychaete had higher growth rates under minimal light conditions. We conclude that UVR and PAR directly control the development of shallow micro- and macrofouling communities by inhibiting the recruitment and growth of sensitive species and promoting the growth of resistant species, but also that these forms of solar radiation influence the surface cues available to competent larvae by altering the development of the microbial community.

  16. Women, men, and rheumatoid arthritis: analyses of disease activity, disease characteristics, and treatments in the QUEST-RA study.

    Science.gov (United States)

    Sokka, Tuulikki; Toloza, Sergio; Cutolo, Maurizio; Kautiainen, Hannu; Makinen, Heidi; Gogus, Feride; Skakic, Vlado; Badsha, Humeira; Peets, Tõnu; Baranauskaite, Asta; Géher, Pál; Ujfalussy, Ilona; Skopouli, Fotini N; Mavrommati, Maria; Alten, Rieke; Pohl, Christof; Sibilia, Jean; Stancati, Andrea; Salaffi, Fausto; Romanowski, Wojciech; Zarowny-Wierzbinska, Danuta; Henrohn, Dan; Bresnihan, Barry; Minnock, Patricia; Knudsen, Lene Surland; Jacobs, Johannes Wg; Calvo-Alen, Jaime; Lazovskis, Juris; Pinheiro, Geraldo da Rocha Castelar; Karateev, Dmitry; Andersone, Daina; Rexhepi, Sylejman; Yazici, Yusuf; Pincus, Theodore

    2009-01-01

    Gender as a predictor of outcomes of rheumatoid arthritis (RA) has evoked considerable interest over the decades. Historically, there is no consensus whether RA is worse in females or males. Recent reports suggest that females are less likely than males to achieve remission. Therefore, we aimed to study possible associations of gender and disease activity, disease characteristics, and treatments of RA in a large multinational cross-sectional cohort of patients with RA called Quantitative Standard Monitoring of Patients with RA (QUEST-RA). The cohort includes clinical and questionnaire data from patients who were seen in usual care, including 6,004 patients at 70 sites in 25 countries as of April 2008. Gender differences were analyzed for American College of Rheumatology Core Data Set measures of disease activity, DAS28 (disease activity score using 28 joint counts), fatigue, the presence of rheumatoid factor, nodules and erosions, and the current use of prednisone, methotrexate, and biologic agents. Women had poorer scores than men in all Core Data Set measures. The mean values for females and males were swollen joint count-28 (SJC28) of 4.5 versus 3.8, tender joint count-28 of 6.9 versus 5.4, erythrocyte sedimentation rate of 30 versus 26, Health Assessment Questionnaire of 1.1 versus 0.8, visual analog scales for physician global estimate of 3.0 versus 2.5, pain of 4.3 versus 3.6, patient global status of 4.2 versus 3.7, DAS28 of 4.3 versus 3.8, and fatigue of 4.6 versus 3.7 (P < 0.001). However, effect sizes were small-medium and smallest (0.13) for SJC28. Among patients who had no or minimal disease activity (0 to 1) on SJC28, women had statistically significantly higher mean values compared with men in all other disease activity measures (P < 0.001) and met DAS28 remission less often than men. Rheumatoid factor was equally prevalent among genders. Men had nodules more often than women. Women had erosions more often than men, but the statistical significance

  17. Quantitative Analyses of Force-Induced Amyloid Formation in Candida albicans Als5p: Activation by Standard Laboratory Procedures.

    Directory of Open Access Journals (Sweden)

    Cho X J Chan

    Full Text Available Candida albicans adhesins have amyloid-forming sequences. In Als5p, these amyloid sequences cluster cell surface adhesins to create high avidity surface adhesion nanodomains. Such nanodomains form after force is applied to the cell surface by atomic force microscopy or laminar flow. Here we report centrifuging and resuspending S. cerevisiae cells expressing Als5p led to 1.7-fold increase in initial rate of adhesion to ligand coated beads. Furthermore, mechanical stress from vortex-mixing of Als5p cells or C. albicans cells also induced additional formation of amyloid nanodomains and consequent activation of adhesion. Vortex-mixing for 60 seconds increased the initial rate of adhesion 1.6-fold. The effects of vortex-mixing were replicated in heat-killed cells as well. Activation was accompanied by increases in thioflavin T cell surface fluorescence measured by flow cytometry or by confocal microscopy. There was no adhesion activation in cells expressing amyloid-impaired Als5pV326N or in cells incubated with inhibitory concentrations of anti-amyloid dyes. Together these results demonstrated the activation of cell surface amyloid nanodomains in yeast expressing Als adhesins, and further delineate the forces that can activate adhesion in vivo. Consequently there is quantitative support for the hypothesis that amyloid forming adhesins act as both force sensors and effectors.

  18. Pulsed activation analyses of the ITER blanket design options considered in the blanket trade-off study

    International Nuclear Information System (INIS)

    Wang, Q.; Henderson, D.L.

    1995-01-01

    Pulsed activation calculations have been performed on two blanket options considered as part of the ITER home team blanket trade-off study. The objective was to compare the activity, afterheat and waste disposal rating (WDR) results of a composite blanket-shield design for the continuous operation approximation to a pulsed operation case to determine whether the differences are at most the duty factor as predicted by the two nuclide chain model. Up to a cooling period of 100 years, the pulsed activity and afterheat values were below the continuous oepration results and well within (except for one afterheat value) the maximum deviation predicted by the two nuclide chain model. No differences in the WDR values were noted as they are, to a large extent, based on long-lived nuclides which are insensitive to short-term changes in the operation history. (orig.)

  19. C9.A/14 steelwork joints de poutres par plaque frontale : assemblages par gousset

    CERN Document Server

    2015-01-01

    Les Tables de résistances ultimes des assemblages boulonnés par plaque frontale et par gousset, complétées par une description des modèles de calcul et des exemples d’application, ont pour but de faciliter la tâche de l'ingénieur et du constructeur. Cette première partie C9.A/14 contient les chapitres suivants: - Joints de poutres par plaque frontale en acier S235 et S355 - Assemblages par gousset en acier S235 et S355 Les Tables contiennent des données relatives à la géométrie ainsi que les valeurs de calcul correspondantes des résistances ultimes des assemblages ; elles remplacent le chapitre « Assemblages par plaques frontales et boulons HR » des anciennes Tables C9.1 de 1983 / 2002. Le calcul de ces assemblages par plaque frontale est basé sur les hypothèses du modèle de la méthode des composants décrite dans la norme SN EN 1993-1-8. Les vérifications sont effectuées selon la norme SIA 263:2013. Les assemblages par gousset remplacent les assemblages par double cornière, (telle...

  20. Toward Enhancing the Enzymatic Activity of a Novel Fungal Polygalacturonase for Food Industry: Optimization and Biochemical Analyses.

    Science.gov (United States)

    Shetaia, Yousseria M H; El-Baz, Ashraf F; ElMekawy, Ahmed M

    2017-08-11

    The review of literature and patents shows that enhancing the PG production and activity are still required to fulfill the increasing demands. A dual optimization process, which involved Plackett-Burman design (PBD), with seven factors, and response surface methodology, was applied to optimize the production of extracellular polygalacturonase (PG) enzyme produced by a novel strain of Aspergillus flavus isolated from rotten orange fruit. The fungal PG was purified and biochemically characterized. Three variables (harvesting time, pH and orange pomace concentration), that were verified to be significant by the PBD analysis, were comprehensively optimized via Box-Behnken design. According to this optimization, the highest PG activity (4073 U/mL) was obtained under pH 7 after 48 h using 40 g/L orange pomace as a substrate, with enhancement in PG activity by 51% compared to the first PBD optimization step. The specific activity of the purified PG was 1608 U/mg with polygalacturonic acid and its molecular weight was 55 kDa. The optimum pH was 5 with relative thermal stability (80%) at 50˚C after 30 min. The PG activity improved in the presence of Cu2+ and Ca2+, while Ba2+, Fe2+ and Zn2+ greatly inhibited the enzyme activity. The obvious Km and Vmax values were 0.8 mg/mL and 2000 µmol/min, respectively. This study is a starting point for initial research in the field of optimization and characterization of A. flavus PG. The statistical optimization of A. flavus PG and its biochemical characterization clearly revealed that this fungal strain can be a potential producer of PG which has a wide range of industrial applications. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.org.

  1. Analyses of antibacterial activity and cell compatibility of titanium coated with a Zr-C-N film.

    Directory of Open Access Journals (Sweden)

    Yin-Yu Chang

    Full Text Available The purpose of this study was to verify the antibacterial performance and cell proliferation activity of zirconium (Zr-carbon (C-nitride (N coatings on commercially pure titanium (Ti with different C contents.Reactive nitrogen gas (N(2 with and without acetylene (C(2H(2 was activated by Zr plasma in a cathodic-arc evaporation system to deposit either a zirconium nitride (ZrN or a Zr-C-N coating onto Ti plates. The bacterial activity of the coatings was evaluated against Staphylococcus aureus with the aid of SYTO9 nucleic acid staining and scanning electron microscopy (SEM. Cell compatibility, mRNA expression, and morphology related to human gingival fibroblasts (HGFs on the coated samples were also determined by using the MTT assay, reverse transcriptase-polymerase chain reaction, and SEM.The Zr-C-N coating with the highest C content (21.7 at% exhibited the lowest bacterial preservation (P<0.001. Biological responses including proliferation, gene expression, and attachment of HGF cells to ZrN and Zr-C-N coatings were comparable to those of the uncoated Ti plate.High-C-content Zr-C-N coatings not only provide short-term antibacterial activity against S. aureus but are also biocompatible with HGF cells.

  2. Biochemical and Structural Analyses of Two Cryptic Esterases in Bacteroides intestinalis and their Synergistic Activities with Cognate Xylanases.

    Science.gov (United States)

    Wefers, Daniel; Cavalcante, Janaina J V; Schendel, Rachel R; Deveryshetty, Jaigeeth; Wang, Kui; Wawrzak, Zdzislaw; Mackie, Roderick I; Koropatkin, Nicole M; Cann, Isaac

    2017-08-04

    Arabinoxylans are constituents of the human diet. Although not utilizable by the human host, they can be fermented by colonic bacteria. The arabinoxylan backbone is decorated with arabinose side chains that may be substituted with ferulic acid, thus limiting depolymerization to fermentable sugars. We investigated the polypeptides encoded by two genes upregulated during growth of the colonic bacterium Bacteroides intestinalis on wheat arabinoxylan. The recombinant proteins, designated BiFae1A and BiFae1B, were functionally assigned esterase activities. Both enzymes were active on acetylated substrates, although each showed a higher ferulic acid esterase activity on methyl-ferulate. BiFae1A showed a catalytic efficiency of 12mM s -1 on para-nitrophenyl-acetate, and on methyl-ferulate, the value was 27 times higher. BiFae1B showed low catalytic efficiencies for both substrates. Furthermore, the two enzymes released ferulic acid from various structural elements, and NMR spectroscopy indicated complete de-esterification of arabinoxylan oligosaccharides from wheat bran. BiFae1A is a tetramer based on the crystal structure, whereas BiFae1B is a dimer in solution based on size exclusion chromatography. The structure of BiFae1A was solved to 1.98Å resolution, and two tetramers were observed in the asymmetric unit. A flexible loop that may act as a hinge over the active site and likely coordinates critical interactions with the substrate was prominent in BiFae1A. Sequence alignments of the esterase domains in BiFae1B with the feruloyl esterase from Clostridium thermocellum suggest that both domains lack the flexible hinge in BiFae1A, an observation that may partly provide a molecular basis for the differences in activities in the two esterases. Copyright © 2017 Elsevier Ltd. All rights reserved.

  3. N-acetylcysteine possesses antidepressant-like activity through reduction of oxidative stress: behavioral and biochemical analyses in rats.

    Science.gov (United States)

    Smaga, Irena; Pomierny, Bartosz; Krzyżanowska, Weronika; Pomierny-Chamioło, Lucyna; Miszkiel, Joanna; Niedzielska, Ewa; Ogórka, Agata; Filip, Małgorzata

    2012-12-03

    The growing body of evidence implicates the significance of oxidative stress in the pathophysiology of depression. The aim of this paper was to examine N-acetylcysteine (NAC) - a putative precursor of the most important tissue antioxidant glutathione - in an animal model of depression and in ex vivo assays to detect oxidative stress parameters. Imipramine (IMI), a classical and clinically-approved antidepressant drug was also under investigation. Male Wistar rats which underwent either bulbectomy (BULB; removal of the olfactory bulbs) or sham surgery (SHAM; olfactory bulbs were left undestroyed) were treated acutely or repeatedly with NAC (50-100mg/kg, ip) or IMI (10mg/kg, ip). Following 10-daily injections with NAC or IMI or their solvents, or 9-daily injections with a corresponding solvent plus acute NAC or acute IMI forced swimming test on day 10, and locomotor activity were performed; immediately after behavioral tests animals were decapitated. Biochemical tests (the total antioxidant capacity - TAC and the superoxide dismutase activity - SOD) were performed on homogenates in several brain structures. In behavioral studies, chronic (but not acute) administration of NAC resulted in a dose-dependent reduction in the immobility time seen only in BULB rats while chronic IMI produced a significant decrease in this parameter in both SHAM and BULB animals. On the other hand, chronic administration of NAC and IMI resulted in a significant increase in cellular antioxidant mechanisms (SOD activity) that reversed the effects of BULB in the frontal cortex, hippocampus and striatum. Our study further supports the antidepressant-like activity of NAC and links its effect as well as IMI actions with the enhancement of brain SOD activity. Copyright © 2012 Elsevier Inc. All rights reserved.

  4. Reading Actively Online: An Exploratory Investigation of Online Annotation Tools for Inquiry Learning / La lecture active en ligne: étude exploratoire sur les outils d'annotation en ligne pour l'apprentissage par l’enquête

    OpenAIRE

    Jingyan Lu; Liping Deng

    2012-01-01

    This study seeks to design and facilitate active reading among secondary school students with an online annotation tool – Diigo. Two classes of different academic performance levels were recruited to examine their annotation behavior and perceptions of Diigo. We wanted to determine whether the two classes differed in how they used Diigo; how they perceived Diigo; and whether how they used Diigo was related to how they perceived it. Using annotation data and surveys in which students reported ...

  5. Adsorption and activation of methane and methanol on Pt(100) surface: a density functional study; Adsorption et activation du methane et du methanol sur la surface (100) du platine: une etude par la fonctionnelle de la densite

    Energy Technology Data Exchange (ETDEWEB)

    Moussounda, P.S

    2006-11-15

    The activation of methane (CH{sub 4}) and methanol (CH{sub 3}OH) on Pt(100) surface has been investigated using density functional theory calculations based on plane-wave basis and pseudo-potential. We optimised CH{sub 4}/Pt(100) system. The calculated adsorption energies over the top, bridge and hollow sites are small, weakly dependent on the molecular orientation. The nature of the CH{sub 4}-Pt interaction was examined through the electronic structure changes. The adsorption of methyl (CH{sub 3}) and hydrogen (H) and the co-adsorption of CH{sub 3}+H were also calculated. From these results, we examined the dissociation of CH{sub 4} to CH{sub 3}+H, and the activation energies found are in good agreement with the experimental and theoretical values. The activation of CH{sub 3}OH/Pt(100) has been studied. All the sites have almost the same adsorption energy. The adsorption of oxygen (O) and the co-adsorption of CH{sub 4} and O were also examined. In addition, the formation of CH{sub 3}OH assuming a one-step mechanism step via the co-adsorption of CH{sub 4}+O has been studied and the barrier height was found to be high. (authors)

  6. Proteinase-activated receptor 4 stimulation-induced epithelial-mesenchymal transition in alveolar epithelial cells

    Directory of Open Access Journals (Sweden)

    Araki Hiromasa

    2007-04-01

    Full Text Available Abstract Background Proteinase-activated receptors (PARs; PAR1–4 that can be activated by serine proteinases such as thrombin and neutrophil catepsin G are known to contribute to the pathogenesis of various pulmonary diseases including fibrosis. Among these PARs, especially PAR4, a newly identified subtype, is highly expressed in the lung. Here, we examined whether PAR4 stimulation plays a role in the formation of fibrotic response in the lung, through alveolar epithelial-mesenchymal transition (EMT which contributes to the increase in myofibroblast population. Methods EMT was assessed by measuring the changes in each specific cell markers, E-cadherin for epithelial cell, α-smooth muscle actin (α-SMA for myofibroblast, using primary cultured mouse alveolar epithelial cells and human lung carcinoma-derived alveolar epithelial cell line (A549 cells. Results Stimulation of PAR with thrombin (1 U/ml or a synthetic PAR4 agonist peptide (AYPGKF-NH2, 100 μM for 72 h induced morphological changes from cobblestone-like structure to elongated shape in primary cultured alveolar epithelial cells and A549 cells. In immunocytochemical analyses of these cells, such PAR4 stimulation decreased E-cadherin-like immunoreactivity and increased α-SMA-like immunoreactivity, as observed with a typical EMT-inducer, tumor growth factor-β (TGF-β. Western blot analyses of PAR4-stimulated A549 cells also showed similar changes in expression of these EMT-related marker proteins. Such PAR4-mediated changes were attenuated by inhibitors of epidermal growth factor receptor (EGFR kinase and Src. PAR4-mediated morphological changes in primary cultured alveolar epithelial cells were reduced in the presence of these inhibitors. PAR4 stimulation increased tyrosine phosphorylated EGFR or tyrosine phosphorylated Src level in A549 cells, and the former response being inhibited by Src inhibitor. Conclusion PAR4 stimulation of alveolar epithelial cells induced epithelial

  7. Experimental study about the regulating effect of Par-4 gene overexpression on the nephroblastoma sensitivity to cisplatin

    Directory of Open Access Journals (Sweden)

    Hui-Lin Mao

    2017-11-01

    Full Text Available Objective: To study the regulating effect of Par-4 gene overexpression on the nephroblastoma sensitivity to cisplatin. Methods: Nephroblastoma SK-NEP-1 cells were cultured and divided into four groups, control group were treated with RMPI-1640 without serum or drugs, cisplatin group were treated with serum-free RMPI-1640 containing 5 μg/mL cisplatin, Par-4 group were transfected by Par-4 overexpression plasmids with serum-free RMPI-1640, and cisplatin + Par-4 group were transfected by Par-4 overexpression plasmid with serum-free RMPI-1640 containing 5 μg/mL cisplatin. The cell proliferation activity as well the expression of apoptosis genes, migration genes and invasion genes was measured. Results: 8 h, 16 h and 24 h after different conditions of treatment, the cell proliferation activity of cisplatin group, Par-4 group and cisplatin + Par-4 group were significantly lower than that of control group, and the cell proliferation activity of cisplatin + Par-4 group was significantly lower than that of cisplatin group and Par-4 group; 24 h after different conditions of treatment, Bim, PDCD4, WT1, RGS4, Axin, KAI1, E-cadherin, PPARγ and PTEN mRNA expression in cisplatin group, Par-4 group and cisplatin + Par-4 group were greatly higher than those in control group whereas GDNF, GFRα1, TUBB3, NME1 and FGF1 mRNA expression were greatly lower than those in control group; Bim, PDCD4, WT1, RGS4, Axin, KAI1, E-cadherin, PPARγ and PTEN mRNA expression in cisplatin + Par-4 group were greatly higher than those in cisplatin group and Par-4 group whereas GDNF, GFRα1, TUBB3, NME1 and FGF1 mRNA expression were greatly significantly lower than those in cisplatin group and Par-4 group. Conclusion: Par-4 gene overexpression can increase the nephroblastoma sensitivity to cisplatin, reduce cell proliferation activity, promote apoptosis and inhibit cell migration and invasion.

  8. MMP-1/PAR-1 signal transduction axis and its prognostic impact in esophageal squamous cell carcinoma

    Directory of Open Access Journals (Sweden)

    Hong-hua Peng

    2012-01-01

    Full Text Available The matrix metalloprotease-1 (MMP-1/protease-activated receptor-1 (PAR-1 signal transduction axis plays an important role in tumorigenesis. To explore the expression and prognostic value of MMP-1 and PAR-1 in esophageal squamous cell carcinoma (ESCC, we evaluated the expression of two proteins in resected specimens from 85 patients with ESCC by immunohistochemistry. Sixty-two (72.9% and 58 (68.2% tumors were MMP-1- and PAR-1-positive, respectively, while no significant staining was observed in normal esophageal squamous epithelium. MMP-1 and PAR-1 overexpression was significantly associated with tumor node metastasis (TNM stage and regional lymph node involvement. Patients with MMP-1- and PAR-1-positive tumors, respectively, had poorer disease-free survival (DFS than those with negative ESCC (P = 0.002 and 0.003, respectively. Univariate analysis showed a significant relationship between TNM stage [hazard ratio (HR = 2.836, 95% confidence interval (CI = 1.866-4.308], regional lymph node involvement (HR = 2.955, 95%CI = 1.713-5.068, MMP-1 expression (HR = 2.669, 95%CI = 1.229-6.127, and PAR-1 expression (HR = 1.762, 95%CI = 1.156-2.883 and DFS. Multivariate analysis including the above four parameters identified TNM stage (HR = 2.035, 95%CI = 1.167-3.681, MMP-1 expression (HR = 2.109, 95%CI = 1.293-3.279, and PAR-1 expression (HR = 1.967, 95%CI = 1.256-2.881 as independent and significant prognostic factors for DFS. Our data suggest for the first time that MMP-1 and PAR-1 were both overexpressed in ESCC and are novel predictors of poor patient prognosis after curative resection. The MMP-1/PAR-1 signal transduction axis might be a new therapeutic target for future therapies tailored against ESCC.

  9. MMP-1/PAR-1 signal transduction axis and its prognostic impact in esophageal squamous cell carcinoma

    Energy Technology Data Exchange (ETDEWEB)

    Peng, Hong-hua; Zhang, Xi; Cao, Pei-guo [Department of Oncology, the Third Xiangya Hospital, Central South University, Changsha, Hunan Province (China)

    2011-11-18

    The matrix metalloprotease-1 (MMP-1)/protease-activated receptor-1 (PAR-1) signal transduction axis plays an important role in tumorigenesis. To explore the expression and prognostic value of MMP-1 and PAR-1 in esophageal squamous cell carcinoma (ESCC), we evaluated the expression of two proteins in resected specimens from 85 patients with ESCC by immunohistochemistry. Sixty-two (72.9%) and 58 (68.2%) tumors were MMP-1- and PAR-1-positive, respectively, while no significant staining was observed in normal esophageal squamous epithelium. MMP-1 and PAR-1 overexpression was significantly associated with tumor node metastasis (TNM) stage and regional lymph node involvement. Patients with MMP-1- and PAR-1-positive tumors, respectively, had poorer disease-free survival (DFS) than those with negative ESCC (P = 0.002 and 0.003, respectively). Univariate analysis showed a significant relationship between TNM stage [hazard ratio (HR) = 2.836, 95% confidence interval (CI) = 1.866-4.308], regional lymph node involvement (HR = 2.955, 95%CI = 1.713-5.068), MMP-1 expression (HR = 2.669, 95%CI = 1.229-6.127), and PAR-1 expression (HR = 1.762, 95%CI = 1.156-2.883) and DFS. Multivariate analysis including the above four parameters identified TNM stage (HR = 2.035, 95%CI = 1.167-3.681), MMP-1 expression (HR = 2.109, 95%CI = 1.293-3.279), and PAR-1 expression (HR = 1.967, 95%CI = 1.256-2.881) as independent and significant prognostic factors for DFS. Our data suggest for the first time that MMP-1 and PAR-1 were both overexpressed in ESCC and are novel predictors of poor patient prognosis after curative resection. The MMP-1/PAR-1 signal transduction axis might be a new therapeutic target for future therapies tailored against ESCC.

  10. Protease-activated receptor 1 and 2 contribute to angiotensin II-induced activation of adventitial fibroblasts from rat aorta

    Energy Technology Data Exchange (ETDEWEB)

    He, Rui-Qing; Tang, Xiao-Feng; Zhang, Bao-Li [State Key Laboratory of Medical Genetics, Shanghai Key Laboratory of Hypertension and Department of Hypertension, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine (China); Shanghai Institute of Hypertension, Shanghai (China); Li, Xiao-Dong [State Key Laboratory of Medical Genetics, Shanghai Key Laboratory of Hypertension and Department of Hypertension, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine (China); Laboratory of Vascular Biology, Institute of Health Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai (China); Shanghai Institute of Hypertension, Shanghai (China); Hong, Mo-Na [State Key Laboratory of Medical Genetics, Shanghai Key Laboratory of Hypertension and Department of Hypertension, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine (China); Shanghai Institute of Hypertension, Shanghai (China); Chen, Qi-Zhi [Shanghai Institute of Hypertension, Shanghai (China); Han, Wei-Qing, E-mail: whan020@gmail.com [State Key Laboratory of Medical Genetics, Shanghai Key Laboratory of Hypertension and Department of Hypertension, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine (China); Laboratory of Vascular Biology, Institute of Health Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai (China); Shanghai Institute of Hypertension, Shanghai (China); Gao, Ping-Jin, E-mail: gaopingjin@sibs.ac.cn [State Key Laboratory of Medical Genetics, Shanghai Key Laboratory of Hypertension and Department of Hypertension, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine (China); Laboratory of Vascular Biology, Institute of Health Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai (China); Shanghai Institute of Hypertension, Shanghai (China)

    2016-04-29

    Adventitial fibroblasts (AFs) can be activated by angiotensin II (Ang II) and exert pro-fibrotic and pro-inflammatory effects in vascular remodeling. Protease-activated receptor (PAR) 1 and 2 play a significant role in fibrogenic and inflammatory diseases. The present study hypothesized that PAR1 and PAR2 are involved in Ang II-induced AF activation and contribute to adventitial remodeling. We found that direct activation of PAR1 and PAR2 with PAR1-AP and PAR2-AP led to AF activation, including proliferation and differentiation of AFs, extracellular matrix synthesis, as well as production of pro-fibrotic cytokine TGF-β and pro-inflammatory cytokines IL-6 and MCP-1. Furthermore, PAR1 and PAR2 mediated Ang II-induced AF activation, since both PAR1 and PAR2 antagonists inhibited Ang II-induced proliferation, migration, differentiation, extracellular matrix synthesis and production of pro-fibrotic and pro-inflammatory cytokines in AFs. Finally, mechanistic study showed that Ang II, via Ang II type I receptor (AT1R), upregulated both PAR1 and PAR2 expression, and transactivated PAR1 and PAR2, as denoted by internalization of both proteins. In conclusion, our results suggest that PAR1 and PAR2 play a critical role in Ang II-induced AF activation, and this may contribute to adventitia-related pathological changes. - Highlights: • Direct activation of PAR1 and PAR2 led to adventitial fibroblast (AF) activation. • PAR1 and PAR2 antagonists attenuated Ang II-induced AF activation. • Ang II induced the upregulation and transactivation of PAR1/PAR2 in AFs.

  11. Protease-activated receptor 1 and 2 contribute to angiotensin II-induced activation of adventitial fibroblasts from rat aorta

    International Nuclear Information System (INIS)

    He, Rui-Qing; Tang, Xiao-Feng; Zhang, Bao-Li; Li, Xiao-Dong; Hong, Mo-Na; Chen, Qi-Zhi; Han, Wei-Qing; Gao, Ping-Jin

    2016-01-01

    Adventitial fibroblasts (AFs) can be activated by angiotensin II (Ang II) and exert pro-fibrotic and pro-inflammatory effects in vascular remodeling. Protease-activated receptor (PAR) 1 and 2 play a significant role in fibrogenic and inflammatory diseases. The present study hypothesized that PAR1 and PAR2 are involved in Ang II-induced AF activation and contribute to adventitial remodeling. We found that direct activation of PAR1 and PAR2 with PAR1-AP and PAR2-AP led to AF activation, including proliferation and differentiation of AFs, extracellular matrix synthesis, as well as production of pro-fibrotic cytokine TGF-β and pro-inflammatory cytokines IL-6 and MCP-1. Furthermore, PAR1 and PAR2 mediated Ang II-induced AF activation, since both PAR1 and PAR2 antagonists inhibited Ang II-induced proliferation, migration, differentiation, extracellular matrix synthesis and production of pro-fibrotic and pro-inflammatory cytokines in AFs. Finally, mechanistic study showed that Ang II, via Ang II type I receptor (AT1R), upregulated both PAR1 and PAR2 expression, and transactivated PAR1 and PAR2, as denoted by internalization of both proteins. In conclusion, our results suggest that PAR1 and PAR2 play a critical role in Ang II-induced AF activation, and this may contribute to adventitia-related pathological changes. - Highlights: • Direct activation of PAR1 and PAR2 led to adventitial fibroblast (AF) activation. • PAR1 and PAR2 antagonists attenuated Ang II-induced AF activation. • Ang II induced the upregulation and transactivation of PAR1/PAR2 in AFs.

  12. Brief communication: Population variation in human maxillary premolar accessory ridges (MxPAR).

    Science.gov (United States)

    Burnett, Scott E; Hawkey, Diane E; Turner, Christy G

    2010-02-01

    The purpose of this brief communication is to report the results of an analysis of maxillary premolar accessory ridges (MxPAR), a common but understudied accessory ridge that may occur both mesial and distal to the central ridge of the buccal cusp of upper premolars. We developed a new five-grade scoring plaque to better categorize MxPAR variation. Subsequently, we conducted a population analysis of MxPAR frequency in 749 dental casts of South African Indian, American Chinese, Alaskan Eskimo, Tohono O'odham (Papago), Akimel O'odham (Pima), Solomon Islander, South African Bantu, and both American and South African Whites. Northeast Asian and Asian-derived populations exhibited the highest MxPAR frequencies while Indo-European samples (South African Indians, American and South African Whites) exhibited relatively low frequencies. The Solomon Islanders and South African Bantu samples exhibited intermediate frequencies. Our analysis indicates that statistically significant differences in MxPAR frequency exist between major geographic populations. As a result, the MxPAR plaque has now been added to the Arizona State University Dental Anthropology System, an important contribution as maxillary premolar traits are underrepresented in analyses of dental morphology. 2009 Wiley-Liss, Inc.

  13. Examining relational empowerment for elementary school students in a yPAR program.

    Science.gov (United States)

    Langhout, Regina Day; Collins, Charles; Ellison, Erin Rose

    2014-06-01

    This paper joins relational empowerment, youth empowerment, and Bridging Multiple Worlds frameworks to examine forms of relational empowerment for children in two intermediary institutions-school and a youth participatory action research after-school program (yPAR ASP). Participants were twelve children, most of whom were Latina/o and from im/migrant families, enrolled in a yPAR ASP for 2 years. A mixed-method approach was utilized; we analyzed children's interviews, self-defined goals, and their social networks to examine their experiences of relational empowerment. We conclude that children experienced each of the five relational empowerment factors-collaborative competence, bridging social divisions, facilitating others' empowerment, mobilizing networks, and passing on a legacy-in the yPAR ASP setting, and some factors in school. These experiences, however, were more pronounced in the yPAR ASP setting. Additionally, social network analyses revealed that a small but meaningful percentage of actors bridged worlds, especially home and family, but by year 2, also school and the yPAR ASP. Finally, most helpers for school-based goals came from school, but a sizable number came from family, friends, and home worlds, and by year 2, also came from the yPAR ASP. Implications range from theoretical to methodological development, including the use of social network analysis as a tool to descriptively examine relational power in context.

  14. Neutron and photon activation analyses in geochemical characterization of sediment profiles at the Jurassic-Cretaceous boundary

    Czech Academy of Sciences Publication Activity Database

    Mizera, Jiří; Řanda, Zdeněk

    2009-01-01

    Roč. 282, č. 1 (2009), s. 53-57 ISSN 0236-5731. [MARC VIII (8th International Conference on Methods and Applications of Radionalytical Chemistry ). Kailua-Kona, Hawai, 05.04.2009-10.04.2009] Institutional research plan: CEZ:AV0Z10480505 Keywords : Jurassic-Cretaceous boundary * Brodno section * Neutron activation analysis Subject RIV: BG - Nuclear, Atomic and Molecular Physics, Colliders Impact factor: 0.631, year: 2009

  15. Evaluation of jaw and neck muscle activities while chewing using EMG-EMG transfer function and EMG-EMG coherence function analyses in healthy subjects.

    Science.gov (United States)

    Ishii, Tomohiro; Narita, Noriyuki; Endo, Hiroshi

    2016-06-01

    This study aims to quantitatively clarify the physiological features in rhythmically coordinated jaw and neck muscle EMG activities while chewing gum using EMG-EMG transfer function and EMG-EMG coherence function analyses in 20 healthy subjects. The chewing side masseter muscle EMG signal was used as the reference signal, while the other jaw (non-chewing side masseter muscle, bilateral anterior temporal muscles, and bilateral anterior digastric muscles) and neck muscle (bilateral sternocleidomastoid muscles) EMG signals were used as the examined signals in EMG-EMG transfer function and EMG-EMG coherence function analyses. Chewing-related jaw and neck muscle activities were aggregated in the first peak of the power spectrum in rhythmic chewing. The gain in the peak frequency represented the power relationships between jaw and neck muscle activities during rhythmic chewing. The phase in the peak frequency represented the temporal relationships between the jaw and neck muscle activities, while the non-chewing side neck muscle presented a broad range of distributions across jaw closing and opening phases. Coherence in the peak frequency represented the synergistic features in bilateral jaw closing muscles and chewing side neck muscle activities. The coherence and phase in non-chewing side neck muscle activities exhibited a significant negative correlation. From above, the bilateral coordination between the jaw and neck muscle activities is estimated while chewing when the non-chewing side neck muscle is synchronously activated with the jaw closing muscles, while the unilateral coordination is estimated when the non-chewing side neck muscle is irregularly activated in the jaw opening phase. Thus, the occurrence of bilateral or unilateral coordinated features in the jaw and neck muscle activities may correspond to the phase characteristics in the non-chewing side neck muscle activities during rhythmical chewing. Considering these novel findings in healthy subjects, EMG

  16. Associations of Fitness, Physical Activity, Strength, and Genetic Risk With Cardiovascular Disease: Longitudinal Analyses in the UK Biobank Study.

    Science.gov (United States)

    Tikkanen, Emmi; Gustafsson, Stefan; Ingelsson, Erik

    2018-04-09

    Background -Observational studies have shown inverse associations among fitness, physical activity, and cardiovascular disease. However, little is known about these associations in individuals with elevated genetic susceptibility for these diseases. Methods -We estimated associations of grip strength, objective and subjective physical activity, and cardiorespiratory fitness with cardiovascular events and all-cause death in a large cohort of 502635 individuals from the UK Biobank (median follow-up, 6.1 years; interquartile range, 5.4-6.8 years). Then we further examined these associations in individuals with different genetic burden by stratifying individuals based on their genetic risk scores for coronary heart disease and atrial fibrillation. We compared disease risk among individuals in different tertiles of fitness, physical activity, and genetic risk using lowest tertiles as reference. Results -Grip strength, physical activity, and cardiorespiratory fitness showed inverse associations with incident cardiovascular events (coronary heart disease: hazard ratio [HR], 0.79; 95% confidence interval [CI], 0.77- 0.81; HR, 0.95; 95% CI, 0.93-0.97; and HR, 0.68; 95% CI, 0.63-0.74, per SD change, respectively; atrial fibrillation: HR, 0.75; 95% CI, 0.73- 0.76; HR, 0.93; 95% CI, 0.91-0.95; and HR, 0.60; 95% CI, 0.56-0.65, per SD change, respectively). Higher grip strength and cardiorespiratory fitness were associated with lower risk of incident coronary heart disease and atrial fibrillation in each genetic risk score group ( P trend fitness were associated with 49% lower risk for coronary heart disease (HR, 0.51; 95% CI, 0.38-0.69) and 60% lower risk for atrial fibrillation (HR, 0.40; 95%, CI 0.30-0.55) among individuals at high genetic risk for these diseases. Conclusions - Fitness and physical activity demonstrated inverse associations with incident cardiovascular disease in the general population, as well as in individuals with elevated genetic risk for these diseases.

  17. Anti-oxidant activity and major chemical component analyses of twenty-six commercially available essential oils

    Directory of Open Access Journals (Sweden)

    Hsiao-Fen Wang

    2017-10-01

    Full Text Available This study analyzed 26 commercially available essential oils and their major chemical components to determine their antioxidant activity levels by measuring their total phenolic content (TPC, reducing power (RP, β-carotene bleaching (BCB activity, trolox equivalent antioxidant capacity (TEAC, and 1,1-diphenyl-2-picrylhydrazyl free radical scavenging (DFRS ability. The clove bud and thyme borneol essential oils had the highest RP, BCB activity levels, and TPC values among the 26 commercial essential oils. Furthermore, of the 26 essential oils, the clove bud and ylang ylang complete essential oils had the highest TEAC values, and the clove bud and jasmine absolute essential oils had the highest DFRS ability. At a concentration of 2.5 mg/mL, the clove bud and thyme borneol essential oils had RP and BCB activity levels of 94.56% ± 0.06% and 24.64% ± 0.03% and 94.58% ± 0.01% and 89.33% ± 0.09%, respectively. At a concentration of 1 mg/mL, the clove bud and thyme borneol essential oils showed TPC values of 220.00 ± 0.01 and 69.05 ± 0.01 mg/g relative to gallic acid equivalents, respectively, and the clove bud and ylang ylang complete essential oils had TEAC values of 809.00 ± 0.01 and 432.33 ± 0.01 μM, respectively. The clove bud and jasmine absolute essential oils showed DFRS abilities of 94.13% ± 0.01% and 78.62% ± 0.01%, respectively. Phenolic compounds of the clove bud, thyme borneol and jasmine absolute essential oils were eugenol (76.08%, thymol (14.36% and carvacrol (12.33%, and eugenol (0.87%, respectively. The phenolic compounds in essential oils were positively correlated with the RP, BCB activity, TPC, TEAC, and DFRS ability.

  18. Anti-oxidant activity and major chemical component analyses of twenty-six commercially available essential oils.

    Science.gov (United States)

    Wang, Hsiao-Fen; Yih, Kuang-Hway; Yang, Chao-Hsun; Huang, Keh-Feng

    2017-10-01

    This study analyzed 26 commercially available essential oils and their major chemical components to determine their antioxidant activity levels by measuring their total phenolic content (TPC), reducing power (RP), β-carotene bleaching (BCB) activity, trolox equivalent antioxidant capacity (TEAC), and 1,1-diphenyl-2-picrylhydrazyl free radical scavenging (DFRS) ability. The clove bud and thyme borneol essential oils had the highest RP, BCB activity levels, and TPC values among the 26 commercial essential oils. Furthermore, of the 26 essential oils, the clove bud and ylang ylang complete essential oils had the highest TEAC values, and the clove bud and jasmine absolute essential oils had the highest DFRS ability. At a concentration of 2.5 mg/mL, the clove bud and thyme borneol essential oils had RP and BCB activity levels of 94.56% ± 0.06% and 24.64% ± 0.03% and 94.58% ± 0.01% and 89.33% ± 0.09%, respectively. At a concentration of 1 mg/mL, the clove bud and thyme borneol essential oils showed TPC values of 220.00 ± 0.01 and 69.05 ± 0.01 mg/g relative to gallic acid equivalents, respectively, and the clove bud and ylang ylang complete essential oils had TEAC values of 809.00 ± 0.01 and 432.33 ± 0.01 μM, respectively. The clove bud and jasmine absolute essential oils showed DFRS abilities of 94.13% ± 0.01% and 78.62% ± 0.01%, respectively. Phenolic compounds of the clove bud, thyme borneol and jasmine absolute essential oils were eugenol (76.08%), thymol (14.36%) and carvacrol (12.33%), and eugenol (0.87%), respectively. The phenolic compounds in essential oils were positively correlated with the RP, BCB activity, TPC, TEAC, and DFRS ability. Copyright © 2017. Published by Elsevier B.V.

  19. Arsenic absorption by members of the Brassicacea family, analysed by neutron activation, k0-method - preliminary results

    International Nuclear Information System (INIS)

    Uemura, George; Matos, Ludmila Vieira da Silva; Silva, Maria Aparecida da; Ferreira, Alexandre Santos Martorano; Menezes, Maria Angela de Barros Correia

    2009-01-01

    Natural arsenic contamination is a cause for concern in many countries of the world including Argentina, Bangladesh, Chile, China, India, Mexico, Thailand and the United States of America and also in Brazil, specially in the Iron Quadrangle area, where mining activities has been contributing to aggravate natural contamination. Brassicacea is a plant family with edible species (arugula, cabbage, cauliflower, cress, kale, mustard, radish), ornamental ones (alysssum, field pennycress, ornamental cabbages and kales) and some species are known as metal and metalloid accumulators (Indian mustard, field pennycress), like chromium, nickel, and arsenic. The present work aimed at studying other taxa of the Brassicaceae family to verify their capability in absorbing arsenic, under controlled conditions, for possible utilisation in remediation activities. The analytical method chosen was neutron activation analysis, k 0 method, a routine technique at CDTN, and also very appropriate for arsenic studies. To avoid possible interference from solid substrates, like sand or vermiculite, attempts were carried out to keep the specimens in 1/4 Murashige and Skoog basal salt solution (M and S). Growth was stumped, plants withered and perished, showing that modifications in M and S had to be done. The addition of nickel and silicon allowed normal growth of the plant specimens, for periods longer than usually achieved (more than two months); yielding samples large enough for further studies with other techniques, like ICP-MS, and other targets, like speciation studies. The results of arsenic absorption are presented here and the need of nickel and silicon in the composition of M and S is discussed. (author)

  20. Arsenic absorption by members of the Brassicacea family, analysed by neutron activation, k{sub 0}-method - preliminary results

    Energy Technology Data Exchange (ETDEWEB)

    Uemura, George; Matos, Ludmila Vieira da Silva; Silva, Maria Aparecida da; Ferreira, Alexandre Santos Martorano; Menezes, Maria Angela de Barros Correia [Centro de Desenvolvimento da Tecnologia Nuclear (CDTN-CNEN/MG), Belo Horizonte, MG (Brazil)], e-mail: george@cdtn.br, e-mail: menezes@cdtn.br

    2009-07-01

    Natural arsenic contamination is a cause for concern in many countries of the world including Argentina, Bangladesh, Chile, China, India, Mexico, Thailand and the United States of America and also in Brazil, specially in the Iron Quadrangle area, where mining activities has been contributing to aggravate natural contamination. Brassicacea is a plant family with edible species (arugula, cabbage, cauliflower, cress, kale, mustard, radish), ornamental ones (alysssum, field pennycress, ornamental cabbages and kales) and some species are known as metal and metalloid accumulators (Indian mustard, field pennycress), like chromium, nickel, and arsenic. The present work aimed at studying other taxa of the Brassicaceae family to verify their capability in absorbing arsenic, under controlled conditions, for possible utilisation in remediation activities. The analytical method chosen was neutron activation analysis, k{sub 0} method, a routine technique at CDTN, and also very appropriate for arsenic studies. To avoid possible interference from solid substrates, like sand or vermiculite, attempts were carried out to keep the specimens in 1/4 Murashige and Skoog basal salt solution (M and S). Growth was stumped, plants withered and perished, showing that modifications in M and S had to be done. The addition of nickel and silicon allowed normal growth of the plant specimens, for periods longer than usually achieved (more than two months); yielding samples large enough for further studies with other techniques, like ICP-MS, and other targets, like speciation studies. The results of arsenic absorption are presented here and the need of nickel and silicon in the composition of M and S is discussed. (author)

  1. Culture-independent analyses reveal novel Anaerolineaceae as abundant primary fermenters in anaerobic digesters treating waste activated sludge

    DEFF Research Database (Denmark)

    McIlroy, Simon Jon; Kirkegaard, Rasmus Hansen; Dueholm, Morten Simonsen

    2017-01-01

    Anaerobic digestion for biogas production is reliant on the tightly coupled synergistic activities of complex microbial consortia. Members of the uncultured A6 phylotype, within the phylum Chloroflexi, are among the most abundant genus-level-taxa of mesophilic anaerobic digester systems treating...... primary and surplus sludge from wastewater treatment plants, yet are known only by their 16S rRNA gene sequence. This study applied metagenomics to obtain a complete circular genome (2.57 Mbp) from a representative of the A6 taxon. Preliminary annotation of the genome indicates these organisms...

  2. Genetic analyses using GGE model and a mixed linear model approach, and stability analyses using AMMI bi-plot for late-maturity alpha-amylase activity in bread wheat genotypes.

    Science.gov (United States)

    Rasul, Golam; Glover, Karl D; Krishnan, Padmanaban G; Wu, Jixiang; Berzonsky, William A; Fofana, Bourlaye

    2017-06-01

    Low falling number and discounting grain when it is downgraded in class are the consequences of excessive late-maturity α-amylase activity (LMAA) in bread wheat (Triticum aestivum L.). Grain expressing high LMAA produces poorer quality bread products. To effectively breed for low LMAA, it is necessary to understand what genes control it and how they are expressed, particularly when genotypes are grown in different environments. In this study, an International Collection (IC) of 18 spring wheat genotypes and another set of 15 spring wheat cultivars adapted to South Dakota (SD), USA were assessed to characterize the genetic component of LMAA over 5 and 13 environments, respectively. The data were analysed using a GGE model with a mixed linear model approach and stability analysis was presented using an AMMI bi-plot on R software. All estimated variance components and their proportions to the total phenotypic variance were highly significant for both sets of genotypes, which were validated by the AMMI model analysis. Broad-sense heritability for LMAA was higher in SD adapted cultivars (53%) compared to that in IC (49%). Significant genetic effects and stability analyses showed some genotypes, e.g. 'Lancer', 'Chester' and 'LoSprout' from IC, and 'Alsen', 'Traverse' and 'Forefront' from SD cultivars could be used as parents to develop new cultivars expressing low levels of LMAA. Stability analysis using an AMMI bi-plot revealed that 'Chester', 'Lancer' and 'Advance' were the most stable across environments, while in contrast, 'Kinsman', 'Lerma52' and 'Traverse' exhibited the lowest stability for LMAA across environments.

  3. Ultrastructural and Molecular Analyses Reveal Enhanced Nucleolar Activity in Medicago truncatula Cells Overexpressing the MtTdp2α Gene

    Science.gov (United States)

    Macovei, Anca; Faè, Matteo; Biggiogera, Marco; de Sousa Araújo, Susana; Carbonera, Daniela; Balestrazzi, Alma

    2018-01-01

    The role of tyrosyl-DNA phosphodiesterase 2 (Tdp2) involved in the repair of 5′-end-blocking DNA lesions is still poorly explored in plants. To gain novel insights, Medicago truncatula suspension cultures overexpressing the MtTdp2α gene (Tdp2α-13C and Tdp2α-28 lines, respectively) and a control (CTRL) line carrying the empty vector were investigated. Transmission electron microscopy (TEM) revealed enlarged nucleoli (up to 44% expansion of the area, compared to CTRL), the presence of nucleolar vacuoles, increased frequency of multinucleolate cells (up to 4.3-fold compared to CTRL) and reduced number of ring-shaped nucleoli in Tdp2α-13C and Tdp2α-28 lines. Ultrastructural data suggesting for enhanced nucleolar activity in MtTdp2α-overexpressing lines were integrated with results from bromouridine incorporation. The latter revealed an increase of labeled transcripts in both Tdp2α-13C and Tdp2α-28 cells, within the nucleolus and in the extra-nucleolar region. MtTdp2α-overexpressing cells showed tolerance to etoposide, a selective inhibitor of DNA topoisomerase II, as evidenced by DNA diffusion assay. TEM analysis revealed etoposide-induced rearrangements within the nucleolus, resembling the nucleolar caps observed in animal cells under transcription impairment. Based on these findings it is evident that MtTdp2α-overexpression enhances nucleolar activity in plant cells. PMID:29868059

  4. Gross alpha and beta activity analyses in urine-a routine laboratory method for internal human radioactivity detection.

    Science.gov (United States)

    Chen, Xiaowen; Zhao, Luqian; Qin, Hongran; Zhao, Meijia; Zhou, Yirui; Yang, Shuqiang; Su, Xu; Xu, Xiaohua

    2014-05-01

    The aim of this work was to develop a method to provide rapid results for humans with internal radioactive contamination. The authors hypothesized that valuable information could be obtained from gas proportional counter techniques by screening urine samples from potentially exposed individuals rapidly. Recommended gross alpha and beta activity screening methods generally employ gas proportional counting techniques. Based on International Standards Organization (ISO) methods, improvements were made in the evaporation process to develop a method to provide rapid results, adequate sensitivity, and minimum sample preparation and operator intervention for humans with internal radioactive contamination. The method described by an American National Standards Institute publication was used to calibrate the gas proportional counter, and urine samples from patients with or without radionuclide treatment were measured to validate the method. By improving the evaporation process, the time required to perform the assay was reduced dramatically. Compared with the reference data, the results of the validation samples were very satisfactory with respect to gross-alpha and gross-beta activities. The gas flow proportional counting method described here has the potential for radioactivity monitoring in the body. This method was easy, efficient, and fast, and its application is of great utility in determining whether a sample should be analyzed by a more complicated method, for example radiochemical and/or γ-spectroscopy. In the future, it may be used commonly in medical examination and nuclear emergency treatment.Health Phys. 106(5):000-000; 2014.

  5. Ultrastructural and Molecular Analyses Reveal Enhanced Nucleolar Activity in Medicago truncatula Cells Overexpressing the MtTdp2α Gene

    Directory of Open Access Journals (Sweden)

    Anca Macovei

    2018-05-01

    Full Text Available The role of tyrosyl-DNA phosphodiesterase 2 (Tdp2 involved in the repair of 5′-end-blocking DNA lesions is still poorly explored in plants. To gain novel insights, Medicago truncatula suspension cultures overexpressing the MtTdp2α gene (Tdp2α-13C and Tdp2α-28 lines, respectively and a control (CTRL line carrying the empty vector were investigated. Transmission electron microscopy (TEM revealed enlarged nucleoli (up to 44% expansion of the area, compared to CTRL, the presence of nucleolar vacuoles, increased frequency of multinucleolate cells (up to 4.3-fold compared to CTRL and reduced number of ring-shaped nucleoli in Tdp2α-13C and Tdp2α-28 lines. Ultrastructural data suggesting for enhanced nucleolar activity in MtTdp2α-overexpressing lines were integrated with results from bromouridine incorporation. The latter revealed an increase of labeled transcripts in both Tdp2α-13C and Tdp2α-28 cells, within the nucleolus and in the extra-nucleolar region. MtTdp2α-overexpressing cells showed tolerance to etoposide, a selective inhibitor of DNA topoisomerase II, as evidenced by DNA diffusion assay. TEM analysis revealed etoposide-induced rearrangements within the nucleolus, resembling the nucleolar caps observed in animal cells under transcription impairment. Based on these findings it is evident that MtTdp2α-overexpression enhances nucleolar activity in plant cells.

  6. Characteristics of diurnal pattern of global photosynthetically-active ...

    African Journals Online (AJOL)

    A two year data (September 1992 August 1994) on photosynhetically-active radiation (PAR) measured at Ilorin (Lat.: 832´N. Long.:434´E) using LI-190SA quantum sensor are analysed both on daily and monthly mean diurnal bases. This was done with the aim of characterizing the diurnal pattern of this radiation at this ...

  7. Indications of human activity from amino acid and amino sugar analyses on Holocene sediments from lake Lonar, central India

    Science.gov (United States)

    Menzel, P.; Gaye, B.; Wiesner, M.; Prasad, S.; Basavaiah, N.; Stebich, M.; Anoop, A.; Riedel, N.; Brauer, A.

    2012-04-01

    The DFG funded HIMPAC (Himalaya: Modern and Past Climates) programme aims to reconstruct Holocene Indian Monsoon climate using a multi-proxy and multi-archive approach. First investigations made on sediments from a ca. 10 m long core covering the whole Holocene taken from the lake Lonar in central India's state Maharashtra, Buldhana District, serve to identify changes in sedimentation, lake chemistry, local vegetation and regional to supra-regional climate patterns. Lake Lonar occupies the floor of an impact crater that formed on the ~ 65 Ma old basalt flows of the Deccan Traps. It covers an area of ca. 1 km2 and is situated in India's core monsoon area. The modern lake has a maximum depth of about 5 m, is highly alkaline, and hyposaline, grouped in the Na-Cl-CO3 subtype of saline lakes. No out-flowing stream is present and only three small streams feed the lake, resulting in a lake level highly sensitive to precipitation and evaporation. The lake is eutrophic and stratified throughout most of the year with sub- to anoxic waters below 2 m depth. In this study the core sediments were analysed for their total amino acid (AA) and amino sugar (AS) content, the amino acid bound C and N percentage of organic C and total N in the sediment and the distribution of individual amino acids. The results roughly show three zones within the core separated by distinct changes in their AA content and distribution. (i) The bottom part of the core from ca. 12000 cal a BP to 11400 cal a BP with very low AA and AS percentage indicating high lithogenic contribution, most probably related to dry conditions. (ii) From 11400 cal a BP to 1200 cal a BP the sediments show moderate AA and AS percentages and low values for the ratios of proteinogenic AAs to their non-proteinogenic degradation products (e.g. ASP/β-ALA; GLU/γ-ABA). (iii) The top part of the core (land use. This hypothesis is corroborated by the dating of more than 10 temple ruins surrounding the lake, which were built in the 12

  8. Analyses of the Photospheric Magnetic Dynamics in Solar Active Region 11117 Using an Advanced CESE-MHD Model

    Energy Technology Data Exchange (ETDEWEB)

    Jiang, Chaowei [SIGMA Weather Group, State Key Laboratory for Space Weather, National Space Science Center, Chinese Academy of Sciences, Beijing (China); Center for Space Plasma and Aeronomic Research, The University of Alabama in Huntsville, Huntsville, AL (United States); Wu, Shi T. [Center for Space Plasma and Aeronomic Research, The University of Alabama in Huntsville, Huntsville, AL (United States); Department of Mechanical and Aerospace Engineering, The University of Alabama in Huntsville, AL (United States); Feng, Xueshang, E-mail: cwjiang@spaceweather.ac.cn [SIGMA Weather Group, State Key Laboratory for Space Weather, National Space Science Center, Chinese Academy of Sciences, Beijing (China)

    2016-05-10

    In this study, the photospheric vector magnetograms obtained by Helioseismic and Magnetic Imager on-board the Solar Dynamics Observatory are used as boundary conditions for a CESE-MHD model to investigate some photosphere characteristics around the time of a confined flare in solar active region NOAA AR 11117. We report our attempt of characterizing a more realistic solar atmosphere by including a plasma with temperature stratified from the photosphere to the corona in the CESE-MHD model. The resulted photospheric transverse flow is comparable to the apparent movements of the magnetic flux features that demonstrates shearing and rotations. We calculated the relevant parameters such as the magnetic energy flux and helicity flux, and with analysis of these parameters, we find that magnetic non-potentiality is transported across the photosphere into the corona in the simulated time interval, which might provide a favorable condition for producing the flare.

  9. Isotopic and enzymatic analyses of planktonic nitrogen utilisation in the vicinity of Cape Sines (Portugal) during weak upwelling activity

    Science.gov (United States)

    Slawyk, Gerd; Coste, Bernard; Collos, Yves; Rodier, Martine

    1997-01-01

    Using measurements of 15N uptake and activities of nitrate reductase and glutamine synthetase, the utilization of nitrogenous nutrients by microplankton in the Portuguese upwelling area was investigated. During this cruise the euphotic zone of coastal waters was in most cases bisected by a nitracline forming two layers. Total inorganic nitrogen uptake rates (NH 4+ + NO 3-) in the upper mixed and nitrate-impoverished layer ranged from 0.1 to 0.8 nM h -1 and were primarily supported by regenerated (ammonium) nitrogen (62-97%), whereas they varied between 0.9 and 10.4 nM h -1 in the deep nitrate-rich layer and were mainly driven by new (nitrate) nitrogen (52-82%). Depth profiles of Chl a-specific uptake rates for ammonium and nitrate paralleled those of absolute uptake rates, i.e. values of VNH 4+Chl were highest (up to 16.1 nmol μg -1 h -1) in nitrate-poor surface waters while values of VNO 3-Chl were maximum (up to 8.4 nmol μg -1 h -1)within the nitracline. This latter vertical ordering of planktonic nitrogen nutrition was consistent with an aged upwelling situation. However, applying several indices of cell metabolism and nutritional status, such as 15N uptake/enzyme activity, surge uptake internally controlled uptake, and V maxChl/K t ratios, we were able to demonstrate that the phytoplankton assemblages inhabiting the nutrient-impoverished upper layer still bore the signature of physically mediated nitrogen (nitrate) supply generated by active upwelling that had occurred during the week before our visit to the area. This signature was the most evident in samples from the station furthest inshore and faded with distance from shore as a result of the deepening of the nitrate isopleths (weakening of upwelling activity), which showed the same offshore trend. The appearance of nitrate-rich waters at the surface, after a strong pulse of upwelling favourable winds just before the end of the cruise, led to a five-fold increase in average (over the euphotic zone

  10. Genotypic analyses of lactobacilli with a range of tannase activities isolated from human feces and fermented foods.

    Science.gov (United States)

    Nishitani, Yosuke; Sasaki, Eiki; Fujisawa, Tomohiko; Osawa, Ro

    2004-02-01

    A total of 77 tannase producing lactobacilli strains isolated from human feces or fermented foods were examined for their genotypic profiles and intensities of tannase production. With a PCR-based assay targeting recA gene, all strains except one isolate were assigned to either Lactobacillus plantarum, L. paraplantarum, or L. pentosus whereas a 16/23S rDNA targeted PCR-based assay identified all except 6 isolates (inclusive of the above one isolate) as one of the closely related species. Subsequent DNA/DNA hybridization assays revealed that these 6 exceptional isolates showed low homology (between 1.2% and 55.8% relative DNA binding) against type strains of the three species. Supplemental carbohydrate fermentation profiles on the 6 isolates indicated that two of them were identified as L. acidophilus, one as Pediococcus acidilactici, one as P. pentosaceus, and two remained unidentifiable. The evidence suggests that the 16/23S rDNA targeted PCR assay can be used as a reliable identification tool for the closely related lactobacilli, and that the tannase gene is widely distributed within members of the Lactobacillaceae family. Meanwhile, a randomly amplified polymorphism DNA (RAPD) analysis revealed that all except 8 isolates were well allocated in 4 major RAPD clusters, though not species specific, consisting of two L. plantarum predominant clusters, one L. paraplantarum predominant, and one L. pentosus predominant. The RAPD patterns of the 8 non-clustered isolates, which consisted of the 6 unidentifiable isolates and 2 isolates identified as L. pentosus, were tannase activities showed that there was a marked variation in the activities among the strains, which did not correlate with either species identification or clustering by RAPD.

  11. Breakthrough in precision (0.3 percent) of neutron activation analyses applied to provenience studies of obsidian

    International Nuclear Information System (INIS)

    Asaro, Frank; Stross, Fred H.; Burger, Richard L.

    2002-01-01

    A gamma ray spectrometer at LBNL (the Luis W. Alvarez Iridium Coincidence Spectrometer), that was specifically designed for high sensitivity measurements of iridium abundances, has been significantly modified in order to provide precisions of measurement in neutron activation analysis of obsidian significantly better than previously obtained (about 1%). Repeated measurements on a single sample of obsidian from a deposit near Chivay, Arequipa, Peru, showed a precision (average coefficient of variation) of 0.19% for the 6 best-measured elements, the value anticipated from the known random errors of measurement. In measurement of samples made from 7 different obsidian nodules from two locations near Chivay, a group of 5 had a spread of 0.30% for the 6 elements measured with counting statistics of better than 0.3% (and 1.8% for the remaining 6 elements). The data suggest there are source inhomogeneity and/or sample preparation contamination errors totaling 0.24 ± .05% for the 6 best measured elements. A sixth obsidian sample could be distinguished from the main group because it differed by +0.8% for most elements, and the last sample could be easily distinguished because several elements differed by more than 1%. The precision of measurements now being developed may provide a significantly more precise determination of the provenience of obsidian artifacts than has been heretofore possible. Also the techniques of measurement developed for obsidian will provide even better precisions with pottery, as many elements are more abundant in pottery than in obsidian

  12. Neutron activation and atomic absorption analyses of heavy metals in corals of Malaysia: historical recorders for sustainable environmental management

    International Nuclear Information System (INIS)

    Mazlin Mokhtar; Almah Awaludin; Abdul Khalik Wood; Lim Kim Shenk; Tan Pey Fang; Yasmin Mohd Hasni

    2001-01-01

    This paper reports the determination of total chromium (Cr), manganese (Mn), and zinc (Zn) in skeletons of the corals Porites sp., sediment and sea water samples taken from various locations in waters of Kedah, Johor and Labuan. Sampling was carried out around Pulau Bunting, Pulau Bidan, Pulau Telur, Pulau Songsong, all in Kedah, Pulau Burung in Labuan, and Pulau Lima in Johor. The concentrations of metals were determined using neutron activation analysis (NAA) and atomic absorption spectrophotometry (AAS). The range of concentrations of Cr, Mn and Zn in coral samples of this study were <0.2 - 1.60, 1.20 - 11.10 and 3.55 - 15.08 μg/g, respectively. Concentrations in sediment samples were Cr 0.62 - 5.19 μg/g, Mn 71.0 - 162 μg/g and Zn 28.2 - 57.8 μg/g. The levels in seawater samples were Cr 0.004 - 0.28 mg/l, Mn 0.26 - 0.33 mg/l, and Zn 0.92 - 1.56 mg/l. (Author)

  13. Decoding of visual activity patterns from fMRI responses using multivariate pattern analyses and convolutional neural network.

    Science.gov (United States)

    Zafar, Raheel; Kamel, Nidal; Naufal, Mohamad; Malik, Aamir Saeed; Dass, Sarat C; Ahmad, Rana Fayyaz; Abdullah, Jafri M; Reza, Faruque

    2017-01-01

    Decoding of human brain activity has always been a primary goal in neuroscience especially with functional magnetic resonance imaging (fMRI) data. In recent years, Convolutional neural network (CNN) has become a popular method for the extraction of features due to its higher accuracy, however it needs a lot of computation and training data. In this study, an algorithm is developed using Multivariate pattern analysis (MVPA) and modified CNN to decode the behavior of brain for different images with limited data set. Selection of significant features is an important part of fMRI data analysis, since it reduces the computational burden and improves the prediction performance; significant features are selected using t-test. MVPA uses machine learning algorithms to classify different brain states and helps in prediction during the task. General linear model (GLM) is used to find the unknown parameters of every individual voxel and the classification is done using multi-class support vector machine (SVM). MVPA-CNN based proposed algorithm is compared with region of interest (ROI) based method and MVPA based estimated values. The proposed method showed better overall accuracy (68.6%) compared to ROI (61.88%) and estimation values (64.17%).

  14. Recognition of Activities of Daily Living Based on Environmental Analyses Using Audio Fingerprinting Techniques: A Systematic Review

    Directory of Open Access Journals (Sweden)

    Ivan Miguel Pires

    2018-01-01

    Full Text Available An increase in the accuracy of identification of Activities of Daily Living (ADL is very important for different goals of Enhanced Living Environments and for Ambient Assisted Living (AAL tasks. This increase may be achieved through identification of the surrounding environment. Although this is usually used to identify the location, ADL recognition can be improved with the identification of the sound in that particular environment. This paper reviews audio fingerprinting techniques that can be used with the acoustic data acquired from mobile devices. A comprehensive literature search was conducted in order to identify relevant English language works aimed at the identification of the environment of ADLs using data acquired with mobile devices, published between 2002 and 2017. In total, 40 studies were analyzed and selected from 115 citations. The results highlight several audio fingerprinting techniques, including Modified discrete cosine transform (MDCT, Mel-frequency cepstrum coefficients (MFCC, Principal Component Analysis (PCA, Fast Fourier Transform (FFT, Gaussian mixture models (GMM, likelihood estimation, logarithmic moduled complex lapped transform (LMCLT, support vector machine (SVM, constant Q transform (CQT, symmetric pairwise boosting (SPB, Philips robust hash (PRH, linear discriminant analysis (LDA and discrete cosine transform (DCT.

  15. Culture-Independent Analyses Reveal Novel Anaerolineaceae as Abundant Primary Fermenters in Anaerobic Digesters Treating Waste Activated Sludge

    Directory of Open Access Journals (Sweden)

    Simon J. McIlroy

    2017-06-01

    Full Text Available Anaerobic digestion for biogas production is reliant on the tightly coupled synergistic activities of complex microbial consortia. Members of the uncultured A6 phylotype, within the phylum Chloroflexi, are among the most abundant genus-level-taxa of mesophilic anaerobic digester systems treating primary and surplus sludge from wastewater treatment plants, yet are known only by their 16S rRNA gene sequence. This study applied metagenomics to obtain a complete circular genome (2.57 Mbp from a representative of the A6 taxon. Preliminary annotation of the genome indicates these organisms to be anaerobic chemoorganoheterotrophs with a fermentative metabolism. Given their observed abundance, they are likely important primary fermenters in digester systems. Application of fluorescence in situ hybridisation probes designed in this study revealed their morphology to be short filaments present within the flocs. The A6 were sometimes co-located with the filamentous Archaea Methanosaeta spp. suggesting potential undetermined synergistic relationships. Based on its genome sequence and morphology we propose the species name Brevefilum fermentans gen. nov. sp. nov.

  16. Breakthrough in precision (0.3 percent) of neutron activation analyses applied to provenience studies of obsidian

    Energy Technology Data Exchange (ETDEWEB)

    Asaro, Frank; Stross, Fred H.; Burger, Richard L.

    2002-10-01

    A gamma ray spectrometer at LBNL (the Luis W. Alvarez Iridium Coincidence Spectrometer), that was specifically designed for high sensitivity measurements of iridium abundances, has been significantly modified in order to provide precisions of measurement in neutron activation analysis of obsidian significantly better than previously obtained (about 1%). Repeated measurements on a single sample of obsidian from a deposit near Chivay, Arequipa, Peru, showed a precision (average coefficient of variation) of 0.19% for the 6 best-measured elements, the value anticipated from the known random errors of measurement. In measurement of samples made from 7 different obsidian nodules from two locations near Chivay, a group of 5 had a spread of 0.30% for the 6 elements measured with counting statistics of better than 0.3% (and 1.8% for the remaining 6 elements). The data suggest there are source inhomogeneity and/or sample preparation contamination errors totaling 0.24 {+-} .05% for the 6 best measured elements. A sixth obsidian sample could be distinguished from the main group because it differed by +0.8% for most elements, and the last sample could be easily distinguished because several elements differed by more than 1%. The precision of measurements now being developed may provide a significantly more precise determination of the provenience of obsidian artifacts than has been heretofore possible. Also the techniques of measurement developed for obsidian will provide even better precisions with pottery, as many elements are more abundant in pottery than in obsidian.

  17. Biochemical and single-molecule analyses of the RNA silencing suppressing activity of CrPV-1A.

    Science.gov (United States)

    Watanabe, Mariko; Iwakawa, Hiro-Oki; Tadakuma, Hisashi; Tomari, Yukihide

    2017-10-13

    Viruses often encode viral silencing suppressors (VSSs) to counteract the hosts' RNA silencing activity. The cricket paralysis virus 1A protein (CrPV-1A) is a unique VSS that binds to a specific Argonaute protein (Ago)-the core of the RNA-induced silencing complex (RISC)-in insects to suppress its target cleavage reaction. However, the precise molecular mechanism of CrPV-1A action remains unclear. Here we utilized biochemical and single-molecule imaging approaches to analyze the effect of CrPV-1A during target recognition and cleavage by Drosophila Ago2-RISC. Our results suggest that CrPV-1A obstructs the initial target searching by Ago2-RISC via base pairing in the seed region. The combination of biochemistry and single-molecule imaging may help to pave the way for mechanistic understanding of VSSs with diverse functions. © The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

  18. Personal, social, and game-related correlates of active and non-active gaming among dutch gaming adolescents : survey-based multivariable, multilevel logistic regression analyses

    NARCIS (Netherlands)

    Simons, Monique; de Vet, Emely; Chinapaw, Mai Jm; de Boer, Michiel; Seidell, Jacob C; Brug, Johannes

    2014-01-01

    BACKGROUND: Playing video games contributes substantially to sedentary behavior in youth. A new generation of video games-active games-seems to be a promising alternative to sedentary games to promote physical activity and reduce sedentary behavior. At this time, little is known about correlates of

  19. Quantitative analyses of impurity silicon-carbide (SiC) and high-purity-titanium by neutron activation analyses based on k0-standardization method. Development of irradiation silicon technology in productivity using research reactor (Joint research)

    International Nuclear Information System (INIS)

    Motohashi, Jun; Takahashi, Hiroyuki; Magome, Hirokatsu; Sasajima, Fumio; Tokunaga, Okihiro; Kawasaki, Kozo; Onizawa, Koji; Isshiki, Masahiko

    2009-07-01

    JRR-3 and JRR-4 have been providing neutron-transmutation-doped silicon (NTD-Si) by using the silicon NTD process, which is a method to produce a high quality semiconductor. The domestic supply of NTD-Si is insufficient for the demand, and the market of NTD-Si is significantly growing at present. It is very important to increase achieve the production. To fulfill the requirement, we have been investigating a neutron filter, which is made of high-purity-titanium, for uniform doping. Silicon-carbide (SiC) semiconductor doped with NTD technology is considered suitable for high power devices with superior performances to conventional Si-based devices. We are very interested in the SiC as well. This report presents the results obtained after the impurity contents in the high-purity-titanium and SiC were analyzed by neutron activation analyses (NAA) using k 0 -standardization method. There were 6 and 9 impurity elements detected from the high-purity-titanium and SiC, respectively. Among those Sc from the high-purity-titanium and Fe from SiC were comparatively long half life nuclides. From the viewpoint of exposure in handling them, we need to examine the impurity control of materials. (author)

  20. Supplemental Performance Analyses for Igneous Activity and Human Intrusion at the Potential High-Level Nuclear Waste Repository at Yucca Mountain

    International Nuclear Information System (INIS)

    Swift, P.; Gaither, K.; Freeze, G.; McCord, J.; Kalinich, D.; Saulnier, G.; Statham, W.

    2002-01-01

    The U.S. Department of Energy (DOE) is considering the possible recommendation of a site at Yucca Mountain, Nevada, for the potential development of a geologic repository for the disposal of high-level radioactive waste and spent nuclear fuel. Consequences of hypothetical disruption of the Yucca Mountain site by igneous activity or human intrusion have been evaluated in the Yucca Mountain Science and Engineering Report (S and ER) (1), which presents technical information supporting the consideration of the possible site recommendation. Since completion of the S and ER, supplemental analyses have examined possible impacts of new information and alternative assumptions on the estimates of the consequences of these events. Specifically, analyses of the consequences of igneous disruption address uncertainty regarding: (1) the impacts of changes in the repository footprint and waste package spacing on the probability of disruption; (2) impacts of alternative assumptions about the appropriate distribution of future wind speeds to use in the analysis; (3) effects of alternative assumptions about waste particle sizes; and (4) alternative assumptions about the number of waste packages damaged by igneous intrusion; and (5) alternative assumptions about the exposure pathways and the biosphere dose conversion factors used in the analysis. Additional supplemental analyses, supporting the Final Environmental Impact Statement (FEIS), have examined the results for both igneous disruption and human intrusion, recalculated for a receptor group located 18 kilometers (km) from the repository (the location specified in 40 CFR 197), rather than at the 20 km distance used in the S and ER analyses

  1. Diversity of the active methanotrophic community in acidic peatlands as assessed by mRNA and SIP-PLFA analyses.

    Science.gov (United States)

    Chen, Yin; Dumont, Marc G; McNamara, Niall P; Chamberlain, Paul M; Bodrossy, Levente; Stralis-Pavese, Nancy; Murrell, J Colin

    2008-02-01

    The active methanotroph community was investigated for the first time in heather (Calluna)-covered moorlands and Sphagnum/Eriophorum-covered UK peatlands. Direct extraction of mRNA from these soils facilitated detection of expression of methane monooxygenase genes, which revealed that particulate methane monooxygenase and not soluble methane monooxygenase was probably responsible for CH(4) oxidation in situ, because only pmoA transcripts (encoding a subunit of particulate methane monooxygenase) were readily detectable. Differences in methanotroph community structures were observed between the Calluna-covered moorland and Sphagnum/Eriophorum-covered gully habitats. As with many other Sphagnum-covered peatlands, the Sphagnum/Eriophorum-covered gullies were dominated by Methylocystis. Methylocella and Methylocapsa-related species were also present. Methylobacter-related species were found as demonstrated by the use of a pmoA-based diagnostic microarray. In Calluna-covered moorlands, in addition to Methylocella and Methylocystis, a unique group of peat-associated type I methanotrophs (Gammaproteobacteria) and a group of uncultivated type II methanotrophs (Alphaproteobacteria) were also found. The pmoA sequences of the latter were only distantly related to Methylocapsa and also to the RA-14 group of methanotrophs, which are believed to be involved in oxidation of atmospheric concentrations of CH(4). Soil samples were also labelled with (13)CH(4), and subsequent analysis of the (13)C-labelled phospholipid fatty acids (PLFAs) showed that 16:1 omega 7, 18:1 omega 7 and 18:1 omega 9 were the major labelled PLFAs. The presence of (13)C-labelled 18:1 omega 9, which was not a major PLFA of any extant methanotrophs, indicated the presence of novel methanotrophs in this peatland.

  2. A multicenter study to standardize reporting and analyses of fluorescence-activated cell-sorted murine intestinal epithelial cells

    Science.gov (United States)

    Magness, Scott T.; Puthoff, Brent J.; Crissey, Mary Ann; Dunn, James; Henning, Susan J.; Houchen, Courtney; Kaddis, John S.; Kuo, Calvin J.; Li, Linheng; Lynch, John; Martin, Martin G.; May, Randal; Niland, Joyce C.; Olack, Barbara; Qian, Dajun; Stelzner, Matthias; Swain, John R.; Wang, Fengchao; Wang, Jiafang; Wang, Xinwei; Yan, Kelley; Yu, Jian

    2013-01-01

    Fluorescence-activated cell sorting (FACS) is an essential tool for studies requiring isolation of distinct intestinal epithelial cell populations. Inconsistent or lack of reporting of the critical parameters associated with FACS methodologies has complicated interpretation, comparison, and reproduction of important findings. To address this problem a comprehensive multicenter study was designed to develop guidelines that limit experimental and data reporting variability and provide a foundation for accurate comparison of data between studies. Common methodologies and data reporting protocols for tissue dissociation, cell yield, cell viability, FACS, and postsort purity were established. Seven centers tested the standardized methods by FACS-isolating a specific crypt-based epithelial population (EpCAM+/CD44+) from murine small intestine. Genetic biomarkers for stem/progenitor (Lgr5 and Atoh 1) and differentiated cell lineages (lysozyme, mucin2, chromogranin A, and sucrase isomaltase) were interrogated in target and control populations to assess intra- and intercenter variability. Wilcoxon's rank sum test on gene expression levels showed limited intracenter variability between biological replicates. Principal component analysis demonstrated significant intercenter reproducibility among four centers. Analysis of data collected by standardized cell isolation methods and data reporting requirements readily identified methodological problems, indicating that standard reporting parameters facilitate post hoc error identification. These results indicate that the complexity of FACS isolation of target intestinal epithelial populations can be highly reproducible between biological replicates and different institutions by adherence to common cell isolation methods and FACS gating strategies. This study can be considered a foundation for continued method development and a starting point for investigators that are developing cell isolation expertise to study physiology and

  3. Analysing deltamethrin susceptibility and pyrethroid esterase activity variations in sylvatic and domestic Triatoma infestans at the embryonic stage

    Directory of Open Access Journals (Sweden)

    Pablo Luis Santo-Orihuela

    2013-12-01

    Full Text Available The aim of the present work was to study the deltamethrin susceptibility of eggs from Triatoma infestans populations and the contribution of pyrethroid esterases to deltamethrin degradation. Insects were collected from sylvatic areas, including Veinte de Octubre and Kirus-Mayu (Bolivia and from domiciliary areas, including El Palmar (Bolivia and La Pista (Argentina. Deltamethrin susceptibility was determined by dose-response bioassays. Serial dilutions of deltamethrin (0.0005-1 mg/mL were topically applied to 12-day-old eggs. Samples from El Palmar had the highest lethal dose ratio (LDR value (44.90 compared to the susceptible reference strain (NFS, whereas the Veinte de Octubre samples had the lowest value (0.50. Pyrethroid esterases were evaluated using 7-coumaryl permethrate (7-CP on individually homogenised eggs from each population and from NFS. The El Palmar and La Pista samples contained 40.11 and 36.64 pmol/min/mg protein, respectively, and these values were statistically similar to NFS (34.92 pmol/min/mg protein and different from Kirus-Mayu and Veinte de Octubre (27.49 and 22.69 pmol/min/mg protein, respectively. The toxicological data indicate that the domestic populations were resistant to deltamethrin, but no statistical contribution of 7-CP esterases was observed. The sylvatic populations had similar LDR values to NFS, but lower 7-CP esterase activities. Moreover, this is the first study of the pyrethroid esterases on T. infestans eggs employing a specific substrate (7-CP.

  4. Principles of Activity Theory in analysing the process of construction of pedagogic activities with the use of mobile devices in the Chemistry learning

    Directory of Open Access Journals (Sweden)

    Liliane da Silva Coelho Jacon

    2014-06-01

    Full Text Available Mobile devices emerge as the major players to ensure a favorable resource to connect, minimizing the limitation space-time constraints among people and enabling the use of emerging mobile learning (m-learning. The use of mobile devices in pedagogic praxis implies in a closer link between teachers in their initial development and their teacher educator in order to enable the incorporation of this mobile technology in undergraduate courses. This “approach” means the facilitation of meeting to discuss, reflect and talk about the incorporation of this technology in the teaching learning process. In this research, two professors had meetings to discuss and reflect about the employment of this mobile technology in the undergraduate course. One of them, a Chemistry teacher educator and the other is a computer and education teacher-research. The methodological approach is based on a qualitative method with some elements of action-research based on theoretical assumptions of the Activity Theory (ENGESTRÖM, 1999. Therefore, the study based on the debates over the use of mobile devices in the teaching of chemistry was developed as part of the undergraduate course in Chemistry at the Federal University of Rondonia. Among a set of activities, in which students and professors were present with their objects of specific activities, was presented the Activity system related to the construction of those activities. The analysis of SA from the perspective of the 5 principles of Activity Theory points out that the process of collaborative participation in the meetings, the implementation of activities with the students of the degree course and the preparation of scientific papers demonstrated the qualitative evolution of the chemistry teacher educator

  5. Comparative proteomic analyses reveal that FlbA down-regulates gliT expression and SOD activity in Aspergillus fumigatus.

    Science.gov (United States)

    Shin, Kwang-Soo; Park, Hee-Soo; Kim, Young-Hwan; Yu, Jae-Hyuk

    2013-07-11

    FlbA is a regulator of G-protein signaling protein that plays a central role in attenuating heterotrimeric G-protein mediated vegetative growth signaling in Aspergillus. The deletion of flbA (∆flbA) in the opportunistic human pathogen Aspergillus fumigatus results in accelerated cell death and autolysis in submerged culture. To further investigate the effects of ∆flbA on intracellular protein levels we carried out 2-D proteome analyses of 2-day old submerged cultures of ∆flbA and wild type (WT) strains and observed 160 differentially expressed proteins. Via nano-LC-ESI-MS/MS analyses, we revealed the identity of 10 and 2 proteins exhibiting high and low level accumulation, respectively, in ∆flbA strain. Notably, the GliT protein is accumulated at about 1800-fold higher levels in ∆flbA than WT. Moreover, GliT is secreted at high levels from ∆flbA strain, whereas Sod1 (superoxide dismutase) is secreted at a higher level in WT. Northern blot analyses reveal that ∆flbA results in elevated accumulation of gliT mRNA. Consequently, ∆flbA strain exhibits enhanced tolerance to gliotoxin toxicity. Finally, ∆flbA strain displayed enhanced SOD activity and elevated resistance to menadione and paraquat. In summary, FlbA-mediated signaling control negatively affects cellular responses associated with detoxification of reactive oxygen species and of exogenous gliotoxin in A. fumigatus. Regulator of G protein Signaling (RGS) proteins play crucial roles in fundamental biological processes in filamentous fungi. FlbA is the first studied filamentous fungal RGS protein, yet much remains to be understood about its roles in the opportunistic human pathogen Aspergillus fumigatus. In the present study, we examined the effects of the deletion of flbA using comprehensive analyses of the intra- and extracellular proteomes of A. fumigatus wild type and the flbA deletion mutant. Via MS analyses, we identified 10 proteins exhibiting high level accumulation in the flbA deletion

  6. PAR-1 mediated apoptosis of breast cancer cells by V. cholerae hemagglutinin protease.

    Science.gov (United States)

    Ray, Tanusree; Pal, Amit

    2016-05-01

    Bacterial toxins have emerged as promising agents in cancer treatment strategy. Hemagglutinin (HAP) protease secreted by Vibrio cholerae induced apoptosis in breast cancer cells and regresses tumor growth in mice model. The success of novel cancer therapies depends on their selectivity for cancer cells with limited toxicity for normal tissues. Increased expression of Protease Activated Receptor-1 (PAR-1) has been reported in different malignant cells. In this study we report that HAP induced activation and over expression of PAR-1 in breast cancer cells (EAC). Immunoprecipitation studies have shown that HAP specifically binds with PAR-1. HAP mediated activation of PAR-1 caused nuclear translocation of p50-p65 and the phosphorylation of p38 which triggered the activation of NFκB and MAP kinase signaling pathways. These signaling pathways enhanced the cellular ROS level in malignant cells that induced the intrinsic pathway of cell apoptosis. PAR-1 mediated apoptosis by HAP of malignant breast cells without effecting normal healthy cells in the same environment makes it a good therapeutic agent for treatment of cancer.

  7. Densité, morphologie urbaine et qualité de vie. Une approche par le projet

    OpenAIRE

    Occhiuto, Rita; Goossens, Marc; Fisher, Axel

    2013-01-01

    La recherche "Densification des tissus urbanisés en Wallonie: forme, acceptabilité et modalités pour accompagner la mutation des tissus bâtis" s'articule en plusieurs volets thématiques, parmi lesquels figure le volet "formes urbaines, modes d'habitat". Ce dernier volet est abordé selon deux approches parallèles et complémentaires qui se valident mutuellement: la première dite "par le projet" (approche qualitative et opérationnelle) et la seconde par analyse statistique et cartographique (ap...

  8. ParA and ParB coordinate chromosome segregation with cell elongation and division during Streptomyces sporulation

    Science.gov (United States)

    Donczew, Magdalena; Mackiewicz, Paweł; Wróbel, Agnieszka; Flärdh, Klas; Zakrzewska-Czerwińska, Jolanta

    2016-01-01

    In unicellular bacteria, the ParA and ParB proteins segregate chromosomes and coordinate this process with cell division and chromosome replication. During sporulation of mycelial Streptomyces, ParA and ParB uniformly distribute multiple chromosomes along the filamentous sporogenic hyphal compartment, which then differentiates into a chain of unigenomic spores. However, chromosome segregation must be coordinated with cell elongation and multiple divisions. Here, we addressed the question of whether ParA and ParB are involved in the synchronization of cell-cycle processes during sporulation in Streptomyces. To answer this question, we used time-lapse microscopy, which allows the monitoring of growth and division of single sporogenic hyphae. We showed that sporogenic hyphae stop extending at the time of ParA accumulation and Z-ring formation. We demonstrated that both ParA and ParB affect the rate of hyphal extension. Additionally, we showed that ParA promotes the formation of massive nucleoprotein complexes by ParB. We also showed that FtsZ ring assembly is affected by the ParB protein and/or unsegregated DNA. Our results indicate the existence of a checkpoint between the extension and septation of sporogenic hyphae that involves the ParA and ParB proteins. PMID:27248800

  9. Contamination potentielle des aliments par des ...

    African Journals Online (AJOL)

    SARA Computers

    la notion de la pollution et de l'exposition aux PCBs, lorsque 77% n'ont pas conscience de cette situation de risque ... 2018). Quoique la contamination par les. PCBs soit de faible niveau, elle est ...... Université Paris-Est, Paris, France ; 183.

  10. Mast cell tryptase stimulates myoblast proliferation; a mechanism relying on protease-activated receptor-2 and cyclooxygenase-2

    Directory of Open Access Journals (Sweden)

    Côté Claude H

    2011-10-01

    Full Text Available Abstract Background Mast cells contribute to tissue repair in fibrous tissues by stimulating proliferation of fibroblasts through the release of tryptase which activates protease-activated receptor-2 (PAR-2. The possibility that a tryptase/PAR-2 signaling pathway exists in skeletal muscle cell has never been investigated. The aim of this study was to evaluate whether tryptase can stimulate myoblast proliferation and determine the downstream cascade. Methods Proliferation of L6 rat skeletal myoblasts stimulated with PAR-2 agonists (tryptase, trypsin and SLIGKV was assessed. The specificity of the tryptase effect was evaluated with a specific inhibitor, APC-366. Western blot analyses were used to evaluate the expression and functionality of PAR-2 receptor and to assess the expression of COX-2. COX-2 activity was evaluated with a commercial activity assay kit and by measurement of PGF2α production. Proliferation assays were also performed in presence of different prostaglandins (PGs. Results Tryptase increased L6 myoblast proliferation by 35% above control group and this effect was completely inhibited by APC-366. We confirmed the expression of PAR-2 receptor in vivo in skeletal muscle cells and in satellite cells and in vitro in L6 cells, where PAR-2 was found to be functional. Trypsin and SLIGKV increased L6 cells proliferation by 76% and 26% above control, respectively. COX-2 activity was increased following stimulation with PAR-2 agonist but its expression remained unchanged. Inhibition of COX-2 activity by NS-398 abolished the stimulation of cell proliferation induced by tryptase and trypsin. Finally, 15-deoxy-Δ-12,14-prostaglandin J2 (15Δ-PGJ2, a product of COX-2-derived prostaglandin D2, stimulated myoblast proliferation, but not PGE2 and PGF2α. Conclusions Taken together, our data show that tryptase can stimulate myoblast proliferation and this effect is part of a signaling cascade dependent on PAR-2 activation and on the downstream

  11. Logit Model of Analysing the Factors Affecting the Adoption of Goat Raising Activity by Farmers in the Non-pastoral Centre Region of Cameroon

    Directory of Open Access Journals (Sweden)

    Folefack, AJZ.

    2018-01-01

    Full Text Available Three years after the beginning of a goat project in the Centre region of Cameroon, the engagement of farmers in this activity has been timid. As this region is not a traditional pastoral zone, farmers have not yet incorporated the crop-livestock integration into their habits. Hence, this paper uses a logistic regression approach in order to analyse the factors affecting the adoption of goat raising activity by farmers of this locality. The computed odds ratio indicate that the practice of goat raising activity is significantly influenced by the farmer's age, gender, farming experience, practice of other livestock activities, frequency of contact with extension agents, access to credit and farm income. However, being a goat raiser does not depend on the farmer's marital status, education, farm size, household size, membership into a common initiative group. The study therefore recommends that the government authorities should give more attention to significant factors so as to popularize the goat raising activity in this region.

  12. CFD analysis of PAR performance as function of inlet design

    Energy Technology Data Exchange (ETDEWEB)

    Park, Kweonha, E-mail: khpark@kmou.ac.kr [Division of Mechanical and Energy systems Engineering, Korea Maritime University, Dongsam-dong, Yeongdo-gu, Busan 606-791 (Korea, Republic of); Khor, Chong Lee, E-mail: itachi_829@hotmail.com [Department of Mechanical Engineering, Korea Maritime University (Korea, Republic of)

    2016-01-15

    Highlights: • The new concept of PAR (passive autocatalytic recombiner) was proposed and analyzed. • Guidance wall was added at the bottom of PAR to enhance the flow rate through the catalyst. • The new concept of PAR was proved to have a better hydrogen removal performance. - Abstract: Passive autocatalytic recombiner (PAR) is very useful hydrogen mitigation measurement. It is widely implemented in the current and advanced light water reactors (ALWRs). The design of the PARs should be optimized for the specific use under severe accident scenarios. Several techniques and innovations have been fused into the PAR, as an effort to increase its efficiency of hydrogen mitigation. This study proposes different concepts of PAR, which applied some changes to the honeycomb catalyst PAR made by the Korea Nuclear Technology (KNT) Inc. Two slices of plate are added to the bottom of PAR model, which intended to act as a reflection wall and promote the gas flow into PAR. Hydrogen volume fraction was given 4 vol. % which tested by KNT to investigate the performance of PAR in different direction gas flow conditions to see maximum hydrogen recombination rate. The new concept of PAR was proved to have a better hydrogen removal performance compared to the original honeycomb catalyst PAR.

  13. CFD analysis of PAR performance as function of inlet design

    International Nuclear Information System (INIS)

    Park, Kweonha; Khor, Chong Lee

    2016-01-01

    Highlights: • The new concept of PAR (passive autocatalytic recombiner) was proposed and analyzed. • Guidance wall was added at the bottom of PAR to enhance the flow rate through the catalyst. • The new concept of PAR was proved to have a better hydrogen removal performance. - Abstract: Passive autocatalytic recombiner (PAR) is very useful hydrogen mitigation measurement. It is widely implemented in the current and advanced light water reactors (ALWRs). The design of the PARs should be optimized for the specific use under severe accident scenarios. Several techniques and innovations have been fused into the PAR, as an effort to increase its efficiency of hydrogen mitigation. This study proposes different concepts of PAR, which applied some changes to the honeycomb catalyst PAR made by the Korea Nuclear Technology (KNT) Inc. Two slices of plate are added to the bottom of PAR model, which intended to act as a reflection wall and promote the gas flow into PAR. Hydrogen volume fraction was given 4 vol. % which tested by KNT to investigate the performance of PAR in different direction gas flow conditions to see maximum hydrogen recombination rate. The new concept of PAR was proved to have a better hydrogen removal performance compared to the original honeycomb catalyst PAR.

  14. Evaluation of Passive Autocatalytic Recombiner (PAR) Implementation in a Konvoi NPP Containment Type

    Energy Technology Data Exchange (ETDEWEB)

    Lopez-Alonso, E.; Papini, D.; Jimenez, G.

    2015-07-01

    The evaluation of Passive Autocatalytic Recombiner (PAR) implementation has been developed under the methodology extracted from the IAEA document, analysing the size, location and number of the PARs capable to minimize the combustion risk, which arises from a hydrogen release generated during a severe accident and its distribution in containment building. A detailed three-dimensional model of Konvoi (PWR) containment with GOTHIC 8.1 code was used for the simulations. The hydrogen preferential pathways and the accumulation points were studied and identified on the basis of a base case scenario without any mitigation measure. The PAR configuration offers an improvement in the chosen accidental scenario; decreasing the possibility of hydrogen combustion and leading to concentration values below the flammability limit (hydrogen concentration below 7%), in all the containment compartments at the end of the transient. Furthermore, from the analysis, it is concluded that the time required to reach hydrogen concentrations below the combustion limit is considerably reduced. (Author)

  15. The differential impact of scientific quality, bibliometric factors, and social media activity on the influence of systematic reviews and meta-analyses about psoriasis.

    Science.gov (United States)

    Ruano, Juan; Aguilar-Luque, Macarena; Gómez-Garcia, Francisco; Alcalde Mellado, Patricia; Gay-Mimbrera, Jesus; Carmona-Fernandez, Pedro J; Maestre-López, Beatriz; Sanz-Cabanillas, Juan Luís; Hernández Romero, José Luís; González-Padilla, Marcelino; Vélez García-Nieto, Antonio; Isla-Tejera, Beatriz

    2018-01-01

    Researchers are increasingly using on line social networks to promote their work. Some authors have suggested that measuring social media activity can predict the impact of a primary study (i.e., whether or not an article will be highly cited). However, the influence of variables such as scientific quality, research disclosures, and journal characteristics on systematic reviews and meta-analyses has not yet been assessed. The present study aims to describe the effect of complex interactions between bibliometric factors and social media activity on the impact of systematic reviews and meta-analyses about psoriasis (PROSPERO 2016: CRD42016053181). Methodological quality was assessed using the Assessing the Methodological Quality of Systematic Reviews (AMSTAR) tool. Altmetrics, which consider Twitter, Facebook, and Google+ mention counts as well as Mendeley and SCOPUS readers, and corresponding article citation counts from Google Scholar were obtained for each article. Metadata and journal-related bibliometric indices were also obtained. One-hundred and sixty-four reviews with available altmetrics information were included in the final multifactorial analysis, which showed that social media and impact factor have less effect than Mendeley and SCOPUS readers on the number of cites that appear in Google Scholar. Although a journal's impact factor predicted the number of tweets (OR, 1.202; 95% CI, 1.087-1.049), the years of publication and the number of Mendeley readers predicted the number of citations in Google Scholar (OR, 1.033; 95% CI, 1.018-1.329). Finally, methodological quality was related neither with bibliometric influence nor social media activity for systematic reviews. In conclusion, there seems to be a lack of connectivity between scientific quality, social media activity, and article usage, thus predicting scientific success based on these variables may be inappropriate in the particular case of systematic reviews.

  16. The differential impact of scientific quality, bibliometric factors, and social media activity on the influence of systematic reviews and meta-analyses about psoriasis

    Science.gov (United States)

    Gómez-Garcia, Francisco; Alcalde Mellado, Patricia; Gay-Mimbrera, Jesus; Carmona-Fernandez, Pedro J.; Maestre-López, Beatriz; Sanz-Cabanillas, Juan Luís; Hernández Romero, José Luís; González-Padilla, Marcelino; Vélez García-Nieto, Antonio; Isla-Tejera, Beatriz

    2018-01-01

    Researchers are increasingly using on line social networks to promote their work. Some authors have suggested that measuring social media activity can predict the impact of a primary study (i.e., whether or not an article will be highly cited). However, the influence of variables such as scientific quality, research disclosures, and journal characteristics on systematic reviews and meta-analyses has not yet been assessed. The present study aims to describe the effect of complex interactions between bibliometric factors and social media activity on the impact of systematic reviews and meta-analyses about psoriasis (PROSPERO 2016: CRD42016053181). Methodological quality was assessed using the Assessing the Methodological Quality of Systematic Reviews (AMSTAR) tool. Altmetrics, which consider Twitter, Facebook, and Google+ mention counts as well as Mendeley and SCOPUS readers, and corresponding article citation counts from Google Scholar were obtained for each article. Metadata and journal-related bibliometric indices were also obtained. One-hundred and sixty-four reviews with available altmetrics information were included in the final multifactorial analysis, which showed that social media and impact factor have less effect than Mendeley and SCOPUS readers on the number of cites that appear in Google Scholar. Although a journal’s impact factor predicted the number of tweets (OR, 1.202; 95% CI, 1.087–1.049), the years of publication and the number of Mendeley readers predicted the number of citations in Google Scholar (OR, 1.033; 95% CI, 1.018–1.329). Finally, methodological quality was related neither with bibliometric influence nor social media activity for systematic reviews. In conclusion, there seems to be a lack of connectivity between scientific quality, social media activity, and article usage, thus predicting scientific success based on these variables may be inappropriate in the particular case of systematic reviews. PMID:29377889

  17. Possibilities of instrumental neutron activation and X-ray fluorescence analyses of sedimentary-magmatic metamorphosed rocks from deep borehole drill cores

    International Nuclear Information System (INIS)

    Gurevich, A.L.; Drynkin, V.I.; Lejpunskaya, D.I.

    1977-01-01

    The possibilities for instrumental neutron-activation and X-ray fluorescence analyses of rocks of metamorphized sedimentary magmatic complexes have been studied with the aid of deep-hole core. The principal characteristics of the conditions of irradiation and of sample measurement ensuring the determination of the content of 26 elements are presented. The use of X-ray fluorescence analysis enables one to determine additionally the content of stron-tium and niobium. Standard specimens of the composition of rocks and complex reference compounds based on phenol formaldehyde resins are used as metrolo.o.ical auxiliaries in the calibration system and in evaluating the correctness of the techniques of instrumental neutron activation and fluorescence analysis. The ranges of the contents to be determined, the sensitivity and relative standard deviation are given. The contribution from the nonuniformity of the specimens to the summary error of element determination is estimated. It is shown that the accuracy and error of analyses are within the allowable range

  18. Applications of quaternary stratigraphic, soil-geomorphic, and quantitative geomorphic analyses to the evaluation of tectonic activity and landscape evolution in the Upper Coastal Plain, South Carolina

    International Nuclear Information System (INIS)

    Hanson, K.L.; Bullard, T.F.; Wit, M.W. de; Stieve, A.L.

    1993-01-01

    Geomorphic analyses combined with mapping of fluvial terraces and upland geomorphic surfaces provide new approaches and data for evaluating the Quaternary activity of post-Cretaceous faults that are recognized in subsurface data at the Savannah River Site in the Upper Coastal Plain of southwestern South Carolina. Analyses of longitudinal stream and terrace profiles, regional slope maps, and drainage basin morphometry indicate long-term uplift and southeast tilt of the site region. Preliminary results of drainage basin characterization suggests an apparent rejuvenation of drainages along the trace of the Pen Branch fault (a Tertiary reactivated reverse fault that initiated as a basin-margin normal fault along the northern boundary of the Triassic Dunbarton Basin). This apparent rejuvenation of drainages may be the result of nontectonic geomorphic processes or local tectonic uplift and tilting within a framework of regional uplift. Longitudinal profiles of fluvial terrace surfaces that are laterally continuous across the projected surface trace of the Pen Branch fault show no obvious evidence of warping or faulting within a resolution of ∼3 m. This combined with the estimated age of the terrace surfaces (350 ka to 1 Ma) indicates that if the Pen Branch fault is active, the Pleistocene rate of slip is very low (0.002 to 0.009 mm/yr)

  19. Non-intrusive measurement of tritium activity in waste drums by modelling a {sup 3}He leak quantified by mass spectrometry; Mesure non intrusive de l'activite de futs de dechets trities par modelisation d'une fuite {sup 3}He et sa quantification par spectrometrie de masse

    Energy Technology Data Exchange (ETDEWEB)

    Demange, D

    2002-07-03

    This study deals with a new method that makes it possible to measure very low tritium quantities inside radioactive waste drums. This indirect method is based on measuring the decaying product, {sup 3}He, and requires a study of its behaviour inside the drum. Our model considers {sup 3}He as totally free and its leak through the polymeric joint of the drum as two distinct phenomena: permeation and laminar flow. The numerical simulations show that a pseudo-stationary state takes place. Thus, the {sup 3}He leak corresponds to the tritium activity inside the drum but it appears, however, that the leak peaks when the atmospheric pressure variations induce an overpressure in the drum. Nevertheless, the confinement of a drum in a tight chamber makes it possible to quantify the {sup 3}He leak. This is a non-intrusive measurement of its activity, which was experimentally checked by using reduced models, representing the drum and its confinement chamber. The drum's confinement was optimised to obtain a reproducible {sup 3}He leak measurement. The gaseous samples taken from the chamber were purified using selective adsorption onto activated charcoals at 77 K to remove the tritium and pre-concentrate the {sup 3}He. The samples were measured using a leak detector mass spectrometer. The adaptation of the signal acquisition and the optimisation of the analysis parameters made it possible to reach the stability of the external calibrations using standard gases with a {sup 3}He detection limit of 0.05 ppb. Repeated confinement of the reference drums demonstrated the accuracy of this method. The uncertainty of this non-intrusive measurement of the tritium activity in 200-liter drums is 15% and the detection limit is about 1 GBq after a 24 h confinement. These results led to the definition of an automated tool able to systematically measure the tritium activity of all storage waste drums. (authors)

  20. Non-intrusive measurement of tritium activity in waste drums by modelling a {sup 3}He leak quantified by mass spectrometry; Mesure non intrusive de l'activite de futs de dechets trities par modelisation d'une fuite {sup 3}He et sa quantification par spectrometrie de masse

    Energy Technology Data Exchange (ETDEWEB)

    Demange, D

    2002-07-03

    This study deals with a new method that makes it possible to measure very low tritium quantities inside radioactive waste drums. This indirect method is based on measuring the decaying product, {sup 3}He, and requires a study of its behaviour inside the drum. Our model considers {sup 3}He as totally free and its leak through the polymeric joint of the drum as two distinct phenomena: permeation and laminar flow. The numerical simulations show that a pseudo-stationary state takes place. Thus, the {sup 3}He leak corresponds to the tritium activity inside the drum but it appears, however, that the leak peaks when the atmospheric pressure variations induce an overpressure in the drum. Nevertheless, the confinement of a drum in a tight chamber makes it possible to quantify the {sup 3}He leak. This is a non-intrusive measurement of its activity, which was experimentally checked by using reduced models, representing the drum and its confinement chamber. The drum's confinement was optimised to obtain a reproducible {sup 3}He leak measurement. The gaseous samples taken from the chamber were purified using selective adsorption onto activated charcoals at 77 K to remove the tritium and pre-concentrate the {sup 3}He. The samples were measured using a leak detector mass spectrometer. The adaptation of the signal acquisition and the optimisation of the analysis parameters made it possible to reach the stability of the external calibrations using standard gases with a {sup 3}He detection limit of 0.05 ppb. Repeated confinement of the reference drums demonstrated the accuracy of this method. The uncertainty of this non-intrusive measurement of the tritium activity in 200-liter drums is 15% and the detection limit is about 1 GBq after a 24 h confinement. These results led to the definition of an automated tool able to systematically measure the tritium activity of all storage waste drums. (authors)

  1. SAFARI 2000 PAR Measurements, Kalahari Transect, Botswana, Wet Season 2000

    Data.gov (United States)

    National Aeronautics and Space Administration — Ceptometer data from a Decagon AccuPAR (Model PAR-80) were collected at four sites in Botswana during the SAFARI 2000 Kalahari Transect Wet Season Campaign (March,...

  2. Évaluation des services rendus par les écosystèmes  en France

    Directory of Open Access Journals (Sweden)

    Philippe Blancher

    2011-12-01

    Full Text Available Cet article rend compte de la méthode d’évaluation des services écosystémiques appliquée au territoire français dans le cadre d’une approche exploratoire. S’inscrivant dans le sillage du ‘‘Millennium Ecosystem Assessment’’ (MA lancé par l’ONU, l’application de la démarche du MA à un territoire donné a conduit à l’élaboration de nouveaux outils d’analyse et de mesure des interactions entre nature et activités humaines à plusieurs échelles territoriales, à travers une projection cartographique des écosystèmes sur l’ensemble du territoire, un recensement des services rendus par les écosystèmes en France et une méthodologie pour leur quantification.This article introduces a method for the assessment of ecosystem services on the French territory. Following the U.N.-initiated Millennium Ecosystem Assessment framework, the implementation of an ecosystem assessment on a specific territory led to the conception of new tools for the analysis and the measurement of interactions between nature and human activities at different territorial scales, through the mapping of ecosystems, an identification of ecosystem services on the French territory and a methodology for their quantification.

  3. Transferred nuclear Overhauser effect analyses of membrane-bound enkephalin analogues by sup 1 H nuclear magnetic resonance: Correlation between activities and membrane-bound conformations

    Energy Technology Data Exchange (ETDEWEB)

    Milon, Alain; Miyazawa, Tatsuo; Higashijima, Tsutomu (Univ. of Tokyo (Japan))

    1990-01-09

    Leu-enkephalin, (D-Ala{sup 2})Leu-enkephalin, and (D-Ala{sup 2})Leu-enkephalinamide (agonists) and (L-Ala{sup 2})Leu-enkephalin (inactive analogue) bind to lipid bilayer consisting of phosphatidylcholine and phosphatidylserine. The conformations that these compounds assume, once bound to perdeuterated phospholipid bilayer, have been shown to be unique, as shown by the transferred nuclear Overhauser effect (TRNOE) of {sup 1}H NMR spectroscopy. In addition, their location in the bilayer was analyzed by TRNOE in the presence of spin-labeled phospholipids. These analyses showed a clear relationship between the activity and the peptide-membrane interaction. The three active peptides, when bound to membranes, adopt the same conformation, characterized by a type II{prime} {beta}-turn around Gly{sup 3}-Phe and a {gamma}-turn around Gly{sup 2} (or D-Ala{sup 2}). The inactive analogue, (L-Ala{sup 2})Leu-enkephalin, displayed a completely different TRNOE pattern corresponding to a different conformation in the membrane-bound state. The tyrosine residue of the active compounds is not inserted into the interior of membrane, but it is inserted into the bilayer for the L-Ala{sup 2} analogue. According to these results, (L-Ala{sup 2})Leu-enkephalin may be explained to be inactive because the mode of binding to the membranes is different from that of active compounds.

  4. Plant-derived SAC domain of PAR-4 (Prostate Apoptosis Response 4 exhibits growth inhibitory effects in prostate cancer cells

    Directory of Open Access Journals (Sweden)

    Shayan eSarkar

    2015-10-01

    Full Text Available The gene Par-4 (Prostate Apoptosis Response 4 was originally identified in prostate cancer cells undergoing apoptosis and its product Par-4 showed cancer specific pro-apoptotic activity. Particularly, the SAC domain of Par-4 (SAC-Par-4 selectively kills cancer cells leaving normal cells unaffected. The therapeutic significance of bioactive SAC-Par-4 is enormous in cancer biology; however, its large scale production is still a matter of concern. Here we report the production of SAC-Par-4-GFP fusion protein coupled to translational enhancer sequence (5′ AMV and apoplast signal peptide (aTP in transgenic Nicotiana tabacum cv. Samsun NN plants under the control of a unique recombinant promoter M24. Transgene integration was confirmed by genomic DNA PCR, Southern and Northern blotting, Real-time PCR and Nuclear run-on assays. Results of Western blot analysis and ELISA confirmed expression of recombinant SAC-Par-4-GFP protein and it was as high as 0.15% of total soluble protein. In addition, we found that targeting of plant recombinant SAC-Par-4-GFP to the apoplast and endoplasmic reticulum (ER was essential for the stability of plant recombinant protein in comparison to the bacterial derived SAC-Par-4. Deglycosylation analysis demonstrated that ER-targeted SAC-Par-4-GFP-SEKDEL undergoes O-linked glycosylation unlike apoplast-targeted SAC-Par-4-GFP. Furthermore, various in vitro studies like mammalian cells proliferation assay (MTT, apoptosis induction assays, and NF-κB suppression suggested the cytotoxic and apoptotic properties of plant-derived SAC-Par-4-GFP against multiple prostate cancer cell lines. Additionally, pre-treatment of MAT-LyLu prostate cancer cells with purified SAC-Par-4-GFP significantly delayed the onset of tumor in a syngeneic rat prostate cancer model. Taken altogether, we proclaim that plant made SAC-Par-4 may become a useful alternate therapy for effectively alleviating cancer in the new era.

  5. Broca Pars Triangularis Constitutes a “Hub” of the Language-Control Network during Simultaneous Language Translation

    Directory of Open Access Journals (Sweden)

    Stefan Elmer

    2016-09-01

    Full Text Available Until now, several branches of research have fundamentally contributed to a better understanding of the ramifications of bilingualism, multilingualism, and language expertise on psycholinguistic-, cognitive-, and neural implications. In this context, it is noteworthy to mention that from a cognitive perspective, there is a strong convergence of data pointing to an influence of multilingual speech competence on a variety of cognitive functions, including attention, short-term- and working memory, set shifting, switching, and inhibition. In addition, complementary neuroimaging findings have highlighted a specific set of cortical and subcortical brain regions which fundamentally contribute to administrate cognitive control in the multilingual brain, namely Broca’s area, the middle-anterior cingulate cortex, the inferior parietal lobe, and the basal ganglia. However, a disadvantage of focusing on group analyses is that this procedure only enables an approximation of the neural networks shared within a population while at the same time smoothing inter-individual differences. In order to address both commonalities (i.e., within group analyses and inter-individual variability (i.e., single-subject analyses in language control mechanisms, here I measured five professional simultaneous interpreters while the participants overtly translated or repeated sentences with a simple subject-verb-object structure. Results demonstrated that pars triangularis was commonly activated across participants during backward translation (i.e., from L2 to L1, whereas the other brain regions of the control network showed a strong inter-individual variability during both backward and forward (i.e., from L1 to L2 translation. Thus, I propose that pars triangularis plays a crucial role within the language-control network and behaves as a fundamental processing entity supporting simultaneous language translation.

  6. Transcriptome, expression, and activity analyses reveal a vital heat shock protein 70 in the stress response of stony coral Pocillopora damicornis.

    Science.gov (United States)

    Zhang, Yidan; Zhou, Zhi; Wang, Lingui; Huang, Bo

    2018-02-12

    Coral bleaching occurs worldwide with increasing frequencies and intensities, which is caused by the stress response of stony coral to environmental change, especially increased sea surface temperature. In the present study, transcriptome, expression, and activity analyses were employed to illustrate the underlying molecular mechanisms of heat shock protein 70 (HSP70) in the stress response of coral to environmental changes. The domain analyses of assembled transcripts revealed 30 HSP70 gene contigs in stony coral Pocillopora damicornis. One crucial HSP70 (PdHSP70) was observed, whose expressions were induced by both elevated temperature and ammonium after expression difference analysis. The complete complementary DNA (cDNA) sequence of PdHSP70 was identified, which encoded a polypeptide of 650 amino acids with a molecular weight of 71.93 kDa. The deduced amino acid sequence of PdHSP70 contained a HSP70 domain (from Pro8 to Gly616), and it shared the highest similarity (95%) with HSP70 from Stylophora pistillata. The expression level of PdHSP70 gene increased significantly at 12 h, and returned to the initial level at 24 h after the stress of high temperature (32 °C). The cDNA fragment encoding the mature peptide of PdHSP70 was recombined and expressed in the prokaryotic expression system. The ATPase activity of recombinant PdHSP70 protein was determined, and it did not change significantly in a wide range of temperature from 25 to 40 °C. These results collectively suggested that PdHSP70 was a vital heat shock protein 70 in the stony coral P. damicornis, whose mRNA expression could be induced by diverse environmental stress and whose activity could remain stable under heat stress. PdHSP70 might be involved in the regulation of the bleaching owing to heat stress in the stony coral P. damicornis.

  7. The inflammatory marker suPAR after cardiac arrest

    DEFF Research Database (Denmark)

    Rundgren, Malin; Lyngbaek, Stig; Fisker, Helle

    2015-01-01

    . This pilot study aimed at investigating suPAR levels in relation to outcome after CA and mild induced hypothermia. METHODS: suPAR levels were measured at 6, 36, and 72 hours in patients treated with hypothermia after CA. suPAR levels were analyzed in relation to survival after 6 months. Receiver operating...

  8. In vitro and in vivo inhibition of proangiogenic retinal phenotype by an antisense oligonucleotide downregulating uPAR expression.

    Science.gov (United States)

    Lulli, Matteo; Cammalleri, Maurizio; Granucci, Irene; Witort, Ewa; Bono, Silvia; Di Gesualdo, Federico; Lupia, Antonella; Loffredo, Rosa; Casini, Giovanni; Dal Monte, Massimo; Capaccioli, Sergio

    2017-08-26

    Neoangiogenesis is the main pathogenic event involved in a variety of retinal diseases. It has been recently demonstrated that inhibiting the urokinase-type plasminogen activator receptor (uPAR) results in reduced angiogenesis in a mouse model of oxygen-induced retinopathy (OIR), establishing uPAR as a therapeutic target in proliferative retinopathies. Here, we evaluated in cultured human retinal endothelial cells (HRECs) and in OIR mice the potential of a specific antisense oligodeoxyribonucleotide (ASO) in blocking the synthesis of uPAR and in providing antiangiogenic effects. uPAR expression in HRECs was inhibited by lipofection with the phosphorotioated 5'-CGGCGGGTGACCCATGTG-3' ASO-uPAR, complementary to the initial translation site of uPAR mRNA. Inhibition of uPAR expression via ASO-uPAR was evaluated in HRECs by analyzing VEGF-induced tube formation and migration. In addition, the well-established and reproducible murine OIR model was used to induce retinal neovascularization in vivo. OIR mice were injected intraperitoneally with ASO-uPAR and retinopathy was evaluated considering the extent of the avascular area in the central retina and neovascular tuft formation. The ASO-uPAR specifically decreased uPAR mRNA and protein levels in HRECs and mitigated VEGF-induced tube formation and cell migration. Noteworthy, in OIR mice ASO-uPAR administration reduced both the avascular area and the formation of neovascular tufts. In conclusion, although the extrapolation of these experimental findings to the clinic is not straightforward, ASO-uPAR may be considered a potential therapeutic tool for treatment of proliferative retinal diseases. Copyright © 2017 Elsevier Inc. All rights reserved.

  9. Phosphorylation of Mycobacterium tuberculosis ParB participates in regulating the ParABS chromosome segregation system.

    Science.gov (United States)

    Baronian, Grégory; Ginda, Katarzyna; Berry, Laurence; Cohen-Gonsaud, Martin; Zakrzewska-Czerwińska, Jolanta; Jakimowicz, Dagmara; Molle, Virginie

    2015-01-01

    Here, we present for the first time that Mycobacterium tuberculosis ParB is phosphorylated by several mycobacterial Ser/Thr protein kinases in vitro. ParB and ParA are the key components of bacterial chromosome segregation apparatus. ParB is a cytosolic conserved protein that binds specifically to centromere-like DNA parS sequences and interacts with ParA, a weak ATPase required for its proper localization. Mass spectrometry identified the presence of ten phosphate groups, thus indicating that ParB is phosphorylated on eight threonines, Thr32, Thr41, Thr53, Thr110, Thr195, and Thr254, Thr300, Thr303 as well as on two serines, Ser5 and Ser239. The phosphorylation sites were further substituted either by alanine to prevent phosphorylation or aspartate to mimic constitutive phosphorylation. Electrophoretic mobility shift assays revealed a drastic inhibition of DNA-binding by ParB phosphomimetic mutant compared to wild type. In addition, bacterial two-hybrid experiments showed a loss of ParA-ParB interaction with the phosphomimetic mutant, indicating that phosphorylation is regulating the recruitment of the partitioning complex. Moreover, fluorescence microscopy experiments performed in the surrogate Mycobacterium smegmatis ΔparB strain revealed that in contrast to wild type Mtb ParB, which formed subpolar foci similar to M. smegmatis ParB, phoshomimetic Mtb ParB was delocalized. Thus, our findings highlight a novel regulatory role of the different isoforms of ParB representing a molecular switch in localization and functioning of partitioning protein in Mycobacterium tuberculosis.

  10. Phosphorylation of Mycobacterium tuberculosis ParB participates in regulating the ParABS chromosome segregation system.

    Directory of Open Access Journals (Sweden)

    Grégory Baronian

    Full Text Available Here, we present for the first time that Mycobacterium tuberculosis ParB is phosphorylated by several mycobacterial Ser/Thr protein kinases in vitro. ParB and ParA are the key components of bacterial chromosome segregation apparatus. ParB is a cytosolic conserved protein that binds specifically to centromere-like DNA parS sequences and interacts with ParA, a weak ATPase required for its proper localization. Mass spectrometry identified the presence of ten phosphate groups, thus indicating that ParB is phosphorylated on eight threonines, Thr32, Thr41, Thr53, Thr110, Thr195, and Thr254, Thr300, Thr303 as well as on two serines, Ser5 and Ser239. The phosphorylation sites were further substituted either by alanine to prevent phosphorylation or aspartate to mimic constitutive phosphorylation. Electrophoretic mobility shift assays revealed a drastic inhibition of DNA-binding by ParB phosphomimetic mutant compared to wild type. In addition, bacterial two-hybrid experiments showed a loss of ParA-ParB interaction with the phosphomimetic mutant, indicating that phosphorylation is regulating the recruitment of the partitioning complex. Moreover, fluorescence microscopy experiments performed in the surrogate Mycobacterium smegmatis ΔparB strain revealed that in contrast to wild type Mtb ParB, which formed subpolar foci similar to M. smegmatis ParB, phoshomimetic Mtb ParB was delocalized. Thus, our findings highlight a novel regulatory role of the different isoforms of ParB representing a molecular switch in localization and functioning of partitioning protein in Mycobacterium tuberculosis.

  11. CFD modelling of hydrogen stratification in enclosures: Model validation and application to PAR performance

    Energy Technology Data Exchange (ETDEWEB)

    Hoyes, J.R., E-mail: james.hoyes@hsl.gsi.gov.uk; Ivings, M.J.

    2016-12-15

    Highlights: • The ability of CFD to predict hydrogen stratification phenomena is investigated. • Contrary to expectation, simulations on tetrahedral meshes under-predict mixing. • Simulations on structured meshes give good agreement with experimental data. • CFD model used to investigate the effects of stratification on PAR performance. • Results show stratification can have a significant effect on PAR performance. - Abstract: Computational Fluid Dynamics (CFD) models are maturing into useful tools for supporting safety analyses. This paper investigates the capabilities of CFD models for predicting hydrogen stratification in a containment vessel using data from the NEA/OECD SETH2 MISTRA experiments. Further simulations are then carried out to illustrate the qualitative effects of hydrogen stratification on the performance of Passive Autocatalytic Recombiner (PAR) units. The MISTRA experiments have well-defined initial and boundary conditions which makes them well suited for use in a validation study. Results are presented for the sensitivity to mesh resolution and mesh type. Whilst the predictions are shown to be largely insensitive to the mesh resolution they are surprisingly sensitive to the mesh type. In particular, tetrahedral meshes are found to induce small unphysical convection currents that result in molecular diffusion and turbulent mixing being under-predicted. This behaviour is not unique to the CFD model used here (ANSYS CFX) and furthermore, it may affect simulations run on other non-aligned meshes (meshes that are not aligned perpendicular to gravity), including non-aligned structured meshes. Following existing best practice guidelines can help to identify potential unphysical predictions, but as an additional precaution consideration should be given to using gravity-aligned meshes for modelling stratified flows. CFD simulations of hydrogen recombination in the Becker Technologies THAI facility are presented with high and low PAR positions

  12. 4G/5G Variant of Plasminogen Activator Inhibitor-1 Gene and Severe Pregnancy-Induced Hypertension: Subgroup Analyses of Variants of Angiotensinogen and Endothelial Nitric Oxide Synthase

    Science.gov (United States)

    Kobashi, Gen; Ohta, Kaori; Yamada, Hideto; Hata, Akira; Minakami, Hisanori; Sakuragi, Noriaki; Tamashiro, Hiko; Fujimoto, Seiichiro

    2009-01-01

    Background Pregnancy-induced hypertension (PIH) is a common cause of perinatal mortality. It is believed to result from the interaction of several factors, including those related to the blood coagulation system. We performed genotyping and subgroup analyses to determine if the 4G/5G genotypes of the plasminogen activator inhibitor-1 gene (PAI-1) play a role in the pathogenesis of PIH, and to evaluate possible interactions of the PAI-1 polymorphisms with those of the angiotensinogen gene (AGT) and the endothelial nitric oxide synthase gene (NOS3). Methods An association study of PAI-1 polymorphism, and subgroup analyses of common variants of AGT and NOS3, among 128 patients with PIH and 376 healthy pregnant controls. Results No significant differences were found between the cases and controls in the frequencies of allele 4G or the 4G/4G genotype. In subgroup analyses, after adjustment for multiple comparison, a significant association with the AGT TT genotype was found among women with the PAI-1 4G/4G genotype, and an association with the NOS3 GA+AA genotype was found among women with the 5G/5G or 4G/5G genotypes. Conclusions Our findings suggest that there are at least 2 pathways in the pathogenesis of severe PIH. However, with respect to early prediction and prevention of severe PIH, although the PAI-1 4G/4G genotype alone was not a risk factor for severe PIH, the fact that PAI-1 genotypes are associated with varying risks for severe PIH suggests that PAI-1 genotyping of pregnant women, in combination with other tests, may be useful in the development of individualized measures that may prevent severe PIH. PMID:19838007

  13. [Ambroise Paré in French literature].

    Science.gov (United States)

    Dumaitre, P

    1995-01-01

    The 16th century by its passionate side has been the favourite one of authors of historical novels in which among the heroes of "cloak and dagger stories" appears sometime Ambroise Paré. Alexandre Dumas (the father) has shown him at the court of Charles IX in La Reine Margot (1845) where he does not however play a great role. On the contrary, Balzac in Le Martyr calviniste (1842) has given him a capital part close to the dying François II, whom he intended to trepanize but had to give up this idea as a consequence of the opposition of the queen-mother Catherine de Médicis. In the present century, Robert Merle in Paris ma bonne ville (Fortune de France, 3, 1980) shows Paré at the time of the Saint Barthélemy.

  14. Correlation between unstimulated salivary flow, pH and streptococcus mutans, analysed with real time PCR, in caries-free and caries-active children.

    Science.gov (United States)

    Abbate, G M; Borghi, D; Passi, A; Levrini, L

    2014-03-01

    Evaluate the correlations between unstimulated salivary flow, pH and level of S. mutans, analysed through real time PCR, in caries-free and caries-active children. Thirty healthy children were divided into 2 groups: test group (DMFT/dmft ≥ 3 and at least 1 active caries lesion) and control group (DMFT/dmft=0). Un-stimulated saliva was collected, pH was measured and S. mutans and total bacterial amount were evaluated with real-time PCR analysis. Unstimulated salivary flow in the test group was significantly lower (p = 0.0269) compared to group control. The level of S. mutans was higher in the test group (p = 0.176), and an inverse correlation was recorded between total bacterial amount and un-stimulated salivary flow (p = 0.063). In the control group a positive relationship was found between total bacterial amount and S. mutans (p = 0.045) and an inverse correlation between pH and S. mutans (p = 0.088). A t-test and a linear regression analysis were performed. A higher salivary flow and an increased salivary pH seem to represent protective factors against caries in children, while high levels of S. mutans are correlated with caries active lesions. Caries risk assessment should be performed considering all parameters involved in the development of the disease.

  15. Continuous analytical control of the streaming waters in a uranium treatment plant and of various chemical products using automatic discharge valves; Controle par analyse en continu des eaux de ruissellement d'une usine traitant de l'uranium et divers produits chimiques avec commande automatique des vannes de decharge

    Energy Technology Data Exchange (ETDEWEB)

    Archimbaud, M; Simeon, C [Commissariat a l' Energie Atomique, Pierrelatte (France)

    1968-07-01

    This report describes a method for controlling the streaming waters produced by the Pierrelatte Centre; it is based on continuous analysis, with simultaneous recording of the species liable to be found accidentally in the corresponding hydrological circuits (chlorides, fluorides, chromium VI, uranium). An alarm set off at pre-determined thresholds leads to an automatic cutting off of the discharge valves; the outward flow of the waters is thus interrupted. This study has shown the various applications which can be found for this water control method, and gives an idea of the cost price. (authors) [French] Ce rapport decrit un mode de controle des eaux de ruissellement provenant du Centre de Pierrelatte base sur une analyse en continu, avec enregistrement des corps susceptibles de se retrouver accidentellement dans les reseaux hydrologiques correspondants (chlorures, fluorures, chrome VI, uranium). Le declenchement d'une alarme a partir de seuils choisis permet de fermer automatiquement les vannes de decharge et d'arreter ainsi l'ecoulement vers l'exterieur. Cette etude montre quelles peuvent etre les diverses applications de cette methode de controle des eaux et elle indique un ordre de grandeur du prix de revient. (auteurs)

  16. Par Pond refill water quality sampling

    International Nuclear Information System (INIS)

    Koch, J.W. II; Martin, F.D.; Westbury, H.M.

    1996-08-01

    This study was designed to document anoxia and its cause in the event that the anoxia caused a fish kill. However, no fish kill was observed during this study, and dissolved oxygen and nutrient concentrations generally remained within the range expected for southeastern reservoirs. Par Pond water quality monitoring will continue during the second summer after refill as the aquatic macrophytes become reestablished and nutrients in the sediments are released to the water column

  17. A method for estimating the diffuse attenuation coefficient (KdPAR)from paired temperature sensors

    Science.gov (United States)

    Read, Jordan S.; Rose, Kevin C.; Winslow, Luke A.; Read, Emily K.

    2015-01-01

    A new method for estimating the diffuse attenuation coefficient for photosynthetically active radiation (KdPAR) from paired temperature sensors was derived. We show that during cases where the attenuation of penetrating shortwave solar radiation is the dominant source of temperature changes, time series measurements of water temperatures at multiple depths (z1 and z2) are related to one another by a linear scaling factor (a). KdPAR can then be estimated by the simple equation KdPAR ln(a)/(z2/z1). A suggested workflow is presented that outlines procedures for calculating KdPAR according to this paired temperature sensor (PTS) method. This method is best suited for conditions when radiative temperature gains are large relative to physical noise. These conditions occur frequently on water bodies with low wind and/or high KdPARs but can be used for other types of lakes during time periods of low wind and/or where spatially redundant measurements of temperatures are available. The optimal vertical placement of temperature sensors according to a priori knowledge of KdPAR is also described. This information can be used to inform the design of future sensor deployments using the PTS method or for campaigns where characterizing sub-daily changes in temperatures is important. The PTS method provides a novel method to characterize light attenuation in aquatic ecosystems without expensive radiometric equipment or the user subjectivity inherent in Secchi depth measurements. This method also can enable the estimation of KdPAR at higher frequencies than many manual monitoring programs allow.

  18. Nondestructive multielement analyses of airborne particulates by combined uses of instrumental neutron activation analysis and energy dispersive X-ray fluorescence analysis

    International Nuclear Information System (INIS)

    Mamuro, Tetsuo; Matsuda, Yatsuka; Mizohata, Akira

    1974-01-01

    Combined uses of instrumental neutron activation analysis and energy dispersive X-ray fluorescence analysis make it possible to analyze nondestructively a considerably large number of elements in airborne particulates. We have confirmed that up to 45 elements can be analyzed without any chemical procedures for urban airborne particulate samples. As the radiation spectrometry by semiconductor detectors and the automatic data reduction by electronic computation are quite common to the two techniques, combined uses of them produce no special annoyance. Several elements can be analyzed by both of them and therefore the reliability of the analytical results can be comfirmed by comparing the data obtained by them with each other. It is noted that this confirmation can be made for the very same sample. In this article are described our experiences of multielement analyses of airborne particulates and some problems to be solved in further studies. (auth.)

  19. Centromere pairing by a plasmid-encoded type I ParB protein

    DEFF Research Database (Denmark)

    Ringgaard, Simon; Löwe, Jan; Gerdes, Kenn

    2007-01-01

    The par2 locus of Escherichia coli plasmid pB171 encodes two trans-acting proteins, ParA and ParB, and two cis-acting sites, parC1 and parC2, to which ParB binds cooperatively. ParA is related to MinD and oscillates in helical structures and thereby positions ParB/parC-carrying plasmids regularly......, hence identifying the N terminus of ParB as a requirement for ParB-mediated centromere pairing. These observations suggest that centromere pairing is an important intermediate step in plasmid partitioning mediated by the common type I loci....

  20. The urokinase receptor (uPAR) and the uPAR-associated protein (uPARAP/Endo180)

    DEFF Research Database (Denmark)

    Behrendt, Niels

    2004-01-01

    The breakdown of the barriers formed by extracellular matrix proteins is a pre-requisite for all processes of tissue remodeling. Matrix degradation reactions take part in specific physiological events in the healthy organism but also represent a crucial step in cancer invasion. These degradation...... on the surface of various cell types that serves to bind the urokinase plasminogen activator and localize the activation reactions in the proteolytic cascade system of plasminogen activation. uPARAP is an integral membrane protein with a pronounced role in the internalization of collagen for intracellular...... degradation. Both receptors have additional functions that are currently being unraveled. The present discussion of uPAR and uPARAP is centered on their protein structure and molecular and cellular function....

  1. Profile inversion of principal diffusivities through the use of a spatially modulated heating and a Fourier analysis; Inversion des profils des diffusivites principales par l'application d'un chauffage spatialement module et une analyse dans le domaine de Fourier

    Energy Technology Data Exchange (ETDEWEB)

    Krapez, J.C.; Spagnolo, L. [Politechnique di Bari (Italy); Friess, M. [Deutsches Luft- und Raumfahrtzentrum eV (DLR), Stuttgart (Germany); Maier, H.P. [Stuttgart Univ., MPA (Germany); Neuer, G. [Institut fur Kernenergetik und Energiesysteme, Universitat Stuttgart (Germany)

    2003-07-01

    The through-thickness thermal diffusivity can be evaluated by the classical flash method. If an homogeneous and extended source is used to irradiate the surface and a thermographic camera is used to monitor the temperature evolution of the opposite side, a map of the through-thickness thermal diffusivity can be obtained in a single experiment and without any contact with the sample under inspection. In order to measure the in-plane thermal diffusivity of a plate-like sample or in one of the principal directions of its plane, a thermal gradient across the plane of the material has to be settled. The ratio of the Fourier transform of temperature at two different spatial frequencies is an exponential function of time multiplied by the diffusivity in the considered principal direction. This can be used to evaluate the diffusivity in an homogenous material. In order to maximize the signal-to-noise ratio, it is better if heat is absorbed over a series of periodic parallel strips (grid flash method). When the material presents a transverse gradient of conductivity, we propose, as a first approach, to perform the Fourier analysis over a sliding window corresponding to one period of the grid pattern. This method allowed us to quantify in situ the diffusivity decrease in a tensile composite sample due to the stress-induced density increase of transverse microcracks. We finally analysed a more rigorous method for transverse conductivity profile inversion. It is based on a perturbation method. The analytical expression of the 'transfer function' between the Fourier transform of the temperature contrast and the Fourier transform of conductivity was established. We validated the proposed inverse technique on simulated and noise-corrupted thermograms. The approach is robust and the simulated profiles are very well resolved. (authors)

  2. Internalisation of the protease-activated receptor 1: role of the third intracellular loop and of the cytoplasmic tail.

    Science.gov (United States)

    Chen, X; Berrou, J; Vigneau, C; Delarue, F; Rondeau, E

    2001-06-01

    To analyse the mechanisms of PAR-1 internalisation, we constructed several PAR-1 mutants and stably expressed them in CHO cells. Our study shows that the Ser(306)-->Ala mutation (S306A), which eliminates a potential site of phosphorylation by PKC in the third intracellular loop of PAR-1, did not change the rate of phosphorylation but reduced the rate of thrombin-induced internalisation of the PAR-1 mutant (58 versus 78% of membrane PAR-1 in 15 min, pinternalisation upon activation. This deletion also inhibited the PMA-induced and the agonist-independent internalisation of the receptor. The Tyr(371)--> Ala mutation (Y371A), in a NPXXY motif of the seventh transmembrane domain of the receptor had no effect on the receptor behaviour. Our results indicate that both the C-tail and the third intracellular loop are involved in PAR-1 internalisation induced by thrombin while only the C-tail plays a role in the PMA-induced and in the agonist-independent PAR-1 internalisation.

  3. Análise de parâmetros funcionais relacionados aos fatores de risco ocupacionais da atividade de enfermeiros de UTI Análisis de parámetros funcionales relacionados a los factores de riesgos laborales de la actividad de enfermeros de UTI Analysis of functional parameters related to occupational risk factors of ICU nursing activity

    Directory of Open Access Journals (Sweden)

    Denise Nery

    2013-03-01

    Full Text Available A avaliação de fatores como fadiga e aspectos funcionais pode identificar sobrecargas durante a atividade de enfermeiros. O objetivo foi avaliar a necessidade de descanso, prevalência de desconfortos musculoesqueléticos, capacidade de trabalho e esforço físico de enfermeiros de Unidade de Terapia Intensiva (UTI. Participaram 24 enfermeiros (idade 40±8 anos; 14 homens e 10 mulheres. Foram aplicados: questionário nórdico de sintomas, índice de capacidade para trabalho (ICT, escala de necessidade de descanso (ENEDE e escala de esforço percebido (Borg. Diferenças entre sexos foram verificadas pelo teste t de Student e na prevalência pelo teste exato de Fisher. Verificou-se prevalência de 75% nos últimos 12 meses, sendo 100% das mulheres e 42% dos homens (diferença significante; p=0,024. As mulheres classificaram o trabalho como mais intenso em comparação aos homens (pLa evaluación de factores como fatiga y aspectos funcionales pueden identificar sobrecargas durante la actividad de los enfermeros. El objetivo fue evaluar la necesidad de descanso, prevalencia de molestias musculoesqueléticas, capacidad de trabajo y esfuerzo físico de enfermeros de UTI. Participaron 24 enfermeros (edad 40±8 años; 14 hombres/10 mujeres. Fueron aplicados: cuestionario nórdico de síntomas, índice de capacidad para el trabajo (ICT, escala de necesidad de descanso (ENEDE y escala de esfuerzo percibido (Borg. Diferencias entre sexos fueron verificadas por el test t de Student y la prevalencia por el test exacto de Fisher. Se verificó la prevalencia del 75% en los últimos 12 meses, siendo el 100% de las mujeres y 42% de los hombres (diferencia significativa; p=0,024. Las mujer